LOCUS       DAKISA010000001        85618 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.001, whole genome shotgun sequence.
ACCESSION   DAKISA010000001 DAKISA010000000
VERSION     DAKISA010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 85618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 85618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85618
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.001"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..160
                     /locus_tag="OVW45_000001"
     CDS             <1..160
                     /locus_tag="OVW45_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCV5524559.1"
                     /translation="WQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYR
                     AIYLRQES"
     gene            complement(198..1259)
                     /locus_tag="OVW45_000002"
     CDS             complement(198..1259)
                     /locus_tag="OVW45_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCV5524560.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            1472..2935
                     /gene="bepA"
                     /locus_tag="OVW45_000003"
     CDS             1472..2935
                     /gene="bepA"
                     /locus_tag="OVW45_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCV5524561.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSQQQARYDARIDQLRQLQERFKPYTKM"
     gene            2956..3315
                     /gene="arsC"
                     /locus_tag="OVW45_000004"
     CDS             2956..3315
                     /gene="arsC"
                     /locus_tag="OVW45_000004"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCV5524562.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKKNGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(3453..4199)
                     /gene="hda"
                     /locus_tag="OVW45_000005"
     CDS             complement(3453..4199)
                     /gene="hda"
                     /locus_tag="OVW45_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCV5524563.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(4249..5538)
                     /gene="uraA"
                     /locus_tag="OVW45_000006"
     CDS             complement(4249..5538)
                     /gene="uraA"
                     /locus_tag="OVW45_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCV5524564.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(5624..6250)
                     /gene="upp"
                     /locus_tag="OVW45_000007"
     CDS             complement(5624..6250)
                     /gene="upp"
                     /locus_tag="OVW45_000007"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCV5524565.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            6575..7612
                     /gene="purM"
                     /locus_tag="OVW45_000008"
     CDS             6575..7612
                     /gene="purM"
                     /locus_tag="OVW45_000008"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCV5524566.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            7612..8250
                     /gene="purN"
                     /locus_tag="OVW45_000009"
     CDS             7612..8250
                     /gene="purN"
                     /locus_tag="OVW45_000009"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCV5524567.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTIRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            8421..10487
                     /gene="ppk1"
                     /locus_tag="OVW45_000010"
     CDS             8421..10487
                     /gene="ppk1"
                     /locus_tag="OVW45_000010"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCV5524568.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            10492..12033
                     /gene="ppx"
                     /locus_tag="OVW45_000011"
     CDS             10492..12033
                     /gene="ppx"
                     /locus_tag="OVW45_000011"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCV5524569.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(12072..14315)
                     /gene="pdeF"
                     /locus_tag="OVW45_000012"
     CDS             complement(12072..14315)
                     /gene="pdeF"
                     /locus_tag="OVW45_000012"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCV5524570.1"
                     /translation="MKLNATYIKMRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYMDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIVPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(14497..14649)
                     /locus_tag="OVW45_000013"
     CDS             complement(14497..14649)
                     /locus_tag="OVW45_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5524571.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            14667..14858
                     /locus_tag="OVW45_000014"
     CDS             14667..14858
                     /locus_tag="OVW45_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCV5524572.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            15169..15687
                     /locus_tag="OVW45_000015"
     CDS             15169..15687
                     /locus_tag="OVW45_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCV5524573.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            15703..16242
                     /locus_tag="OVW45_000016"
     CDS             15703..16242
                     /locus_tag="OVW45_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCV5524574.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLTLAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16337..17914)
                     /gene="guaA"
                     /locus_tag="OVW45_000017"
     CDS             complement(16337..17914)
                     /gene="guaA"
                     /locus_tag="OVW45_000017"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCV5524575.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(17983..19449)
                     /gene="guaB"
                     /locus_tag="OVW45_000018"
     CDS             complement(17983..19449)
                     /gene="guaB"
                     /locus_tag="OVW45_000018"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCV5524576.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            19611..20981
                     /gene="xseA"
                     /locus_tag="OVW45_000019"
     CDS             19611..20981
                     /gene="xseA"
                     /locus_tag="OVW45_000019"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCV5524577.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSCNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(20978..21193)
                     /locus_tag="OVW45_000020"
     CDS             complement(20978..21193)
                     /locus_tag="OVW45_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCV5524578.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(21262..22734)
                     /gene="der"
                     /locus_tag="OVW45_000021"
     CDS             complement(21262..22734)
                     /gene="der"
                     /locus_tag="OVW45_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCV5524579.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLERVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDTNVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(22852..24030)
                     /gene="bamB"
                     /locus_tag="OVW45_000022"
     CDS             complement(22852..24030)
                     /gene="bamB"
                     /locus_tag="OVW45_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCV5524580.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(24041..24661)
                     /gene="yfgM"
                     /locus_tag="OVW45_000023"
     CDS             complement(24041..24661)
                     /gene="yfgM"
                     /locus_tag="OVW45_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCV5524581.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGKALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(24679..25953)
                     /gene="hisS"
                     /locus_tag="OVW45_000024"
     CDS             complement(24679..25953)
                     /gene="hisS"
                     /locus_tag="OVW45_000024"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCV5524582.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(26064..27182)
                     /gene="ispG"
                     /locus_tag="OVW45_000025"
                     /gene_synonym="gcpE"
     CDS             complement(26064..27182)
                     /gene="ispG"
                     /locus_tag="OVW45_000025"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCV5524583.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(27209..28222)
                     /gene="rodZ"
                     /locus_tag="OVW45_000026"
     CDS             complement(27209..28222)
                     /gene="rodZ"
                     /locus_tag="OVW45_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCV5524584.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(28507..29661)
                     /gene="trmG/rlmN"
                     /locus_tag="OVW45_000027"
     CDS             complement(28507..29661)
                     /gene="trmG/rlmN"
                     /locus_tag="OVW45_000027"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCV5524585.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(29811..30242)
                     /gene="ndk"
                     /locus_tag="OVW45_000028"
     CDS             complement(29811..30242)
                     /gene="ndk"
                     /locus_tag="OVW45_000028"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCV5524586.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(30391..32703)
                     /gene="pbpC"
                     /locus_tag="OVW45_000029"
     CDS             complement(30391..32703)
                     /gene="pbpC"
                     /locus_tag="OVW45_000029"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HCV5524587.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAAQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEG
                     INGIRFPIWLDDKGQRVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(32704..37665)
                     /gene="yfhM"
                     /locus_tag="OVW45_000030"
     CDS             complement(32704..37665)
                     /gene="yfhM"
                     /locus_tag="OVW45_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HCV5524588.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRNVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGLRPQFALK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLQADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LSVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGIALKTMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLVTLQVSNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     VGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            37872..38717
                     /gene="sseA"
                     /locus_tag="OVW45_000031"
     CDS             37872..38717
                     /gene="sseA"
                     /locus_tag="OVW45_000031"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HCV5524589.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            complement(39210..39986)
                     /gene="sseB"
                     /locus_tag="OVW45_000032"
     CDS             complement(39210..39986)
                     /gene="sseB"
                     /locus_tag="OVW45_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCV5524590.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(40128..41411)
                     /gene="pepB"
                     /locus_tag="OVW45_000033"
     CDS             complement(40128..41411)
                     /gene="pepB"
                     /locus_tag="OVW45_000033"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCV5524591.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(41589..41789)
                     /gene="iscX"
                     /locus_tag="OVW45_000034"
     CDS             complement(41589..41789)
                     /gene="iscX"
                     /locus_tag="OVW45_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCV5524592.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(41801..42136)
                     /gene="fdx"
                     /locus_tag="OVW45_000035"
     CDS             complement(41801..42136)
                     /gene="fdx"
                     /locus_tag="OVW45_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCV5524593.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(42138..43988)
                     /gene="hscA"
                     /locus_tag="OVW45_000036"
     CDS             complement(42138..43988)
                     /gene="hscA"
                     /locus_tag="OVW45_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCV5524594.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(44005..44520)
                     /gene="hscB"
                     /locus_tag="OVW45_000037"
     CDS             complement(44005..44520)
                     /gene="hscB"
                     /locus_tag="OVW45_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCV5524595.1"
                     /translation="MDYFTFFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(44616..44939)
                     /gene="iscA"
                     /locus_tag="OVW45_000038"
     CDS             complement(44616..44939)
                     /gene="iscA"
                     /locus_tag="OVW45_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCV5524596.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(44956..45342)
                     /gene="iscU"
                     /locus_tag="OVW45_000039"
     CDS             complement(44956..45342)
                     /gene="iscU"
                     /locus_tag="OVW45_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCV5524597.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(45370..46584)
                     /gene="iscS"
                     /locus_tag="OVW45_000040"
     CDS             complement(45370..46584)
                     /gene="iscS"
                     /locus_tag="OVW45_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCV5524598.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(46825..47313)
                     /gene="iscR"
                     /locus_tag="OVW45_000041"
     CDS             complement(46825..47313)
                     /gene="iscR"
                     /locus_tag="OVW45_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCV5524599.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(47614..48351)
                     /gene="trmJ"
                     /locus_tag="OVW45_000042"
     CDS             complement(47614..48351)
                     /gene="trmJ"
                     /locus_tag="OVW45_000042"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCV5524600.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            48470..49273
                     /gene="suhB"
                     /locus_tag="OVW45_000043"
     CDS             48470..49273
                     /gene="suhB"
                     /locus_tag="OVW45_000043"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCV5524601.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYSQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            49418..50272
                     /locus_tag="OVW45_000044"
     CDS             49418..50272
                     /locus_tag="OVW45_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCV5524602.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGLGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKETKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            50463..51743
                     /gene="csiE"
                     /locus_tag="OVW45_000045"
     CDS             50463..51743
                     /gene="csiE"
                     /locus_tag="OVW45_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCV5524603.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(51735..52874)
                     /locus_tag="OVW45_000046"
     CDS             complement(51735..52874)
                     /locus_tag="OVW45_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCV5524604.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAVRQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(53034..53924)
                     /gene="hcaR"
                     /locus_tag="OVW45_000047"
     CDS             complement(53034..53924)
                     /gene="hcaR"
                     /locus_tag="OVW45_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCV5524605.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            54060..55421
                     /locus_tag="OVW45_000048"
     CDS             54060..55421
                     /locus_tag="OVW45_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCV5524606.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            55418..55936
                     /locus_tag="OVW45_000049"
     CDS             55418..55936
                     /locus_tag="OVW45_000049"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCV5524607.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLEQLDKEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            55936..56256
                     /locus_tag="OVW45_000050"
     CDS             55936..56256
                     /locus_tag="OVW45_000050"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCV5524608.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            56253..57065
                     /gene="hcaB"
                     /locus_tag="OVW45_000051"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             56253..57065
                     /gene="hcaB"
                     /locus_tag="OVW45_000051"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCV5524609.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAARYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            57075..58277
                     /locus_tag="OVW45_000052"
     CDS             57075..58277
                     /locus_tag="OVW45_000052"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HCV5524610.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIAIGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            58374..58796
                     /locus_tag="OVW45_000053"
     CDS             58374..58796
                     /locus_tag="OVW45_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCV5524611.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(58844..59716)
                     /locus_tag="OVW45_000054"
     CDS             complement(58844..59716)
                     /locus_tag="OVW45_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCV5524612.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(59728..60822)
                     /locus_tag="OVW45_000055"
     CDS             complement(59728..60822)
                     /locus_tag="OVW45_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCV5524613.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(60855..61853)
                     /locus_tag="OVW45_000056"
     CDS             complement(60855..61853)
                     /locus_tag="OVW45_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCV5524614.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(61878..63389)
                     /locus_tag="OVW45_000057"
     CDS             complement(61878..63389)
                     /locus_tag="OVW45_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCV5524615.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(63412..64395)
                     /locus_tag="OVW45_000058"
     CDS             complement(63412..64395)
                     /locus_tag="OVW45_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCV5524616.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTLGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLNKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(64492..67769)
                     /locus_tag="OVW45_000059"
                     /pseudo
     CDS             complement(64492..67769)
                     /locus_tag="OVW45_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            67887..69080
                     /locus_tag="OVW45_000060"
                     /pseudo
     CDS             67887..69080
                     /locus_tag="OVW45_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            complement(69278..70531)
                     /gene="glyA"
                     /locus_tag="OVW45_000061"
     CDS             complement(69278..70531)
                     /gene="glyA"
                     /locus_tag="OVW45_000061"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCV5524617.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARFPVYA"
     gene            70859..72049
                     /gene="hmpA"
                     /locus_tag="OVW45_000062"
     CDS             70859..72049
                     /gene="hmpA"
                     /locus_tag="OVW45_000062"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCV5524618.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWHRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(72094..72432)
                     /gene="glnB"
                     /locus_tag="OVW45_000063"
     CDS             complement(72094..72432)
                     /gene="glnB"
                     /locus_tag="OVW45_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCV5524619.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(72493..73827)
                     /gene="glrR"
                     /locus_tag="OVW45_000064"
     CDS             complement(72493..73827)
                     /gene="glrR"
                     /locus_tag="OVW45_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCV5524620.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(73817..74530)
                     /gene="qseG"
                     /locus_tag="OVW45_000065"
     CDS             complement(73817..74530)
                     /gene="qseG"
                     /locus_tag="OVW45_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCV5524621.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPTGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(74695..76122)
                     /gene="qseE"
                     /locus_tag="OVW45_000066"
     CDS             complement(74695..76122)
                     /gene="qseE"
                     /locus_tag="OVW45_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCV5524622.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(76680..80567)
                     /gene="purL"
                     /locus_tag="OVW45_000067"
                     /gene_synonym="purI"
     CDS             complement(76680..80567)
                     /gene="purL"
                     /locus_tag="OVW45_000067"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCV5524623.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNTLLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            80825..82381
                     /gene="mltF"
                     /locus_tag="OVW45_000068"
     CDS             80825..82381
                     /gene="mltF"
                     /locus_tag="OVW45_000068"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCV5524624.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVSQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(82378..82914)
                     /gene="tadA"
                     /locus_tag="OVW45_000069"
     CDS             complement(82378..82914)
                     /gene="tadA"
                     /locus_tag="OVW45_000069"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCV5524625.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQPSTD"
     gene            complement(82939..83574)
                     /gene="yfhb"
                     /locus_tag="OVW45_000070"
     CDS             complement(82939..83574)
                     /gene="yfhb"
                     /locus_tag="OVW45_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCV5524626.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            83783..84631
                     /locus_tag="OVW45_000071"
     CDS             83783..84631
                     /locus_tag="OVW45_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCV5524627.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(84970..85527)
                     /gene="pinE"
                     /locus_tag="OVW45_000072"
                     /pseudo
     CDS             complement(84970..85527)
                     /gene="pinE"
                     /locus_tag="OVW45_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
BASE COUNT        20774 a        23275 c        21428 g        20141 t
ORIGIN      
        1 ctggcaactg gcgcacagta gcgcagacaa ttttccggca ctgacggtaa gccattttat
       61 taccgccaat ctgctcccgg tgatgctggg taatattatc ggcggtgcag tgctggtgag
      121 tatgtgttat cgggctattt atttacgtca ggaatcctga aaaatcagcc cggcgaaaca
      181 gttcgtcggg ctgaagatta ttcttgcgct atttgcccat cgggccaggc gtggatcacg
      241 gctttgatca gcgtcgccaa tggaatagcg aagaatacgc cccagaatcc ccacaaacca
      301 ccgaagatca ccaccgataa aataatcacc agcggatgca ggttaaccgc ttcggagaac
      361 aacaccggta ctaacagatt accgtccagc gcctgaataa tcagatacac tgcgaagcag
      421 ctccagaatt ccgtgcctgc accaaactgg aatagcgcca cgccaaccac cggaatggtc
      481 accacaaatg cgccaatgta cggaataagt accgagaagc cgaccagcac cgccagcagc
      541 agcgaatagt tcagcccaaa gagcaagaac cccagccaag tggcgatccc caccacgatc
      601 atctccagca ctttgccgcg gatatagttg gtgatttgtt gattcatctc cttccacacc
      661 tgtcctgcca gtccacggtt gcgcggcagc acccgacgaa cggcgttcag catctgctct
      721 ttgtctttca gcaggaagaa gaccatcaat ggtaccagca ccagatagac ggcaatggtc
      781 agcaaaccga ccagcgaggc gagggaaatt ttcaccaccg aatcgcccat ggtcagcatc
      841 cgactgcgca tattttcggc cattgcatca ataatgcccg cgtccattaa tgccggatag
      901 cggcgcggca acgtggcggc aaagtcagaa agcttattga gcatccccgg catatcgcgg
      961 atcaagtaga tgccctgttg ccaggcgata ggcagtacca cgaacgccat cagtagcaat
     1021 ataccgacga aaaccaccaa tacaatcgac gtcgcccagc ggcgggagca gccaatagat
     1081 tgcaggcgca cggttggcca ttccagcaaa taggccagca caatagccac cagcaacgga
     1141 gcaagcaggc cactaaagaa aaagataatg ccaaatccgg caactaaaat aaccagcaag
     1201 gcaatcgctt ccgggtcgct aaaacggcgg cgataccatt gcatcaacat ttcgagcata
     1261 aagaccttcc ctgaacctca agagcgggat tgcgatccgc aattgtatcg aaatgtcaca
     1321 aaaaagactt cgctttttat gacggattca ggaaactgaa aagtcatttg agtgggctaa
     1381 tcttcgccgt tacactcaaa ggcggcgcgg tgggaacaat atttcacagt atcggtcaaa
     1441 tgactatctt cagaaataca ggatagaggt tatgttcagg cagttgaaaa aaaacctggt
     1501 tgcaaccctc attgctgcta tgaccattgg tcaggtagcc ccggcgtttg ccgacagcgc
     1561 agacaccttg ccggatatgg gaacctccgc aggaagcacg ctttccattg gtcaggaaat
     1621 gcagatgggc gactattatg tccgccagct acgcggcagc gcgccgttaa ttaatgaccc
     1681 gctgttaacg caatatatta attcgctggg gatgcgtctg gtttcgcatg ccaattcggt
     1741 taagacaccg tttcattttt ttctgatcaa caacgacgaa attaacgcct ttgctttctt
     1801 tggcggcaac gtggtgctgc actctgccct gttccgttat tccgataacg aaagtcaact
     1861 ggcttcagtt atggcgcacg aaatctccca cgtcacccaa cgtcacctgg cgcgagcgat
     1921 ggaagatcag cagcgcagcg cgccgctgac ctgggtcggc gcgttaggtt ctattttact
     1981 ggcgatggcc agtccgcagg cggggatggc ggcgctgacc ggtacactgg cgggaacgcg
     2041 tcaggggatg atcagtttca cccagcaaaa tgaacaggaa gcggaccgca ttggtattca
     2101 ggtgctgcaa cgctcgggat tcgatccgca ggcgatgcca accttcctcg aaaaattact
     2161 cgatcaggcg cgttactcct cgcgcccgcc ggaaatttta ttgactcacc cgttgccgga
     2221 aagtcgtctg gcagatgccc gcaaccgtgc taatcagatg cgcccgatgg tggtgcagtc
     2281 gtcggaagat ttctatctgg cgaaagcgcg cacactgggg atgtataatt ccggacgtaa
     2341 ccagctcacc agtgatttgc tggatgaatg ggcgaaagga aacgttcgtc agcaacgagc
     2401 ggcgcaatat ggtcgtgctt tacaggcgat ggaagccaat aaatacgacg aggcgcgtaa
     2461 aacgctgcaa ccgttactgg cggcagaacc tggcaacgca tggtatctcg atctggctac
     2521 cgatatcgat cttgggcaaa acaaagccaa tgaggcaatc aatcgcctga aaaatgcccg
     2581 tgatttgcgc accaatccgg tgttgcagct caacctggca aacgcttatc tgcaaggcgg
     2641 tcaaccacaa gaagcggcca atattctgaa tcgctacacc tttaataata aagatgacag
     2701 caacggctgg gatttactgg cacaggcgga agccgcgcta aataaccgcg atcaggagct
     2761 ggctgcgcga gcagaaggtt atgcgctcgc cggacgactc gatcaggcca tttcgctgtt
     2821 gagtagcgcc agttcgcagg tgaaattagg cagccagcaa caagcgcgtt acgatgcgcg
     2881 catcgaccag ttgcgccagc tgcaggaacg ctttaagcct tataccaaga tgtaattcgc
     2941 attaaaggaa gaacaatgac caaacaggta aaaatctacc ataacccacg ctgttcaaag
     3001 agccgggaaa cgctgaacct gctgaaaaaa aacggcgtgg agccagaagt ggtactctac
     3061 cttgagacac ccgccgatgc ggcaacgctg cgcgatttgc tgaaaatgct ggggatgaat
     3121 agcgcccgtg aattgatgcg ccagaaagag gatctttata aagaactgaa cctggctgac
     3181 agttcgctta gcgaagaggc gctgattcag gcaatggtgg ataatccgaa gctgatggag
     3241 cgcccgattg tggtcgcgaa tggcaaagcg cggattggtc gtccaccgga gcaggtactg
     3301 gaaatcgtgg gttaagatgt gccccatcgc tagttgaagc acattgccgg atgcgacgct
     3361 tgccatgtct tatccggcct acgaatacta tggattaact tcgcgtagtt cggataaggc
     3421 gttcgcgccg catccgacaa taaacacctt atctacaact tcagaatttc tttcacaaac
     3481 ggaatggtca gcttacgttg cgcggtaatc gacgcacgat ccaactgatc caacgtcata
     3541 aatagcgtgc gcatttctct gtcgagccgc ttcagcaaga aacgccccac atcttccggc
     3601 agttcaaaac cacgcaaacg cgcgcgtaac tgtagcgcct gcaacttatc ttcatcagaa
     3661 agtggctgca atttgtagat ctgcccccag tcgagtcgcg acgcgagatc cggtaatccc
     3721 agattcaact gccgcggtgg acgatcgccg gtgatcaaca atcgtgtttt gcccgattcc
     3781 agaattcgat tgtagagatc gaaaatcgcc atctcccaca actcatcgcc tgcaatacac
     3841 tcaatgttgt cgatacagac cagcgacaaa tgctccatac cgtcgagcac ttccggaaca
     3901 aaccaggtgc gtttatccag cgggacatag cccaccgcat cgccacgctg cgacaattcc
     3961 gcgcaagccg cgtgcagcag atggctgcgc cccgcgcctt cgcgtgccca gagatagatg
     4021 taaccgctat gttcctgacg cagcacgttt tgcagcgcag ccagtaaaga ggagttatcc
     4081 cccggccaga aacttgcaaa ggtttcgtcg tcaggaagat aaagtggcaa agagagctgt
     4141 gccggtgtgt tcagagatac ctcaaccagg atttcacaaa atcgcgagaa gtttaccaca
     4201 gaatcccatg atgtttgaac cgggcagcaa cactgcccgg tcggtacatt atttgtctgt
     4261 tatatccgcg tcttctgcgt ccagcaccac ttcttcaggg cgcagcacac taatcagttt
     4321 gaagatcaaa cttaagccga taccgacgat ggtcgccagc gccatacctt tcagctccgc
     4381 cgcgccaatg tttaccttcg cgccactgac accgatgatc aaaatcacgg aagtcaggat
     4441 caggttctgt gctttgttgt aatccacttt tgattcgatc aaaacacgaa taccggaagc
     4501 accgatgaca ccataaagca gcagcgaaac gccgcccatc accggcaatg ggatcatctg
     4561 gatagcggca gccagtttac cgacgcagga aagcaggata gcgaaaatcg ccgccccacc
     4621 gataacccag gtactgtaaa cacgggtgat cgccatcacg ccaatgtttt ctccgtaagt
     4681 ggtatttggc gtagagccaa agaagccgga gatcacagtc gacaggccgt tagcaaacat
     4741 cgaacggtgc agacctggat cgcgcagcag atcttttttg acgatattag ccgttactac
     4801 caggtgccct acgtgttcgg caataaccac taacgccgct ggcagaatag tcagaatggc
     4861 aaaccactcg aagcgcggcg tatagagggt tggcagcgca aaccagtgag cattaataat
     4921 cggcgtggta tcgacaattc ccattgcgaa agagagcgcg taccccacca gcacgccaat
     4981 taaaatcggg ataattgcca agaaaccacg gaacagcacg gaacctaaaa ccgtgaccgc
     5041 cagggtggta atagagatga tgatggtttt ggagtctggc gtttgccctt cagccgggag
     5101 taaacccgcc ataccggcag ctacgcccgc cagctccaga ccgatgacgg caacgattgc
     5161 gcccattgcc gcaggtggaa acagcacgtc cagccagccg gtccccgctt tcttcacgat
     5221 aaaagaaacc aggcagaaca gcacgccgca cataataaag ccgcccagcg cgacttcata
     5281 ccctaacggc aacagtaaca ataccggtga aataaaggca aagctggaac caagataagc
     5341 cggaattttc cctttacaga tgaagagata cagcagcgtt ccaataccgt taaataacag
     5401 tacagtcgcc gggttaatat gaaataagac gggcaccagg acggttgcac caaacatggc
     5461 gaacaaatgt tgcaaactaa gcgggattgt ctgtaaaagt ggcggtcttt cactcacccc
     5521 gatagcacgg cgcgtcatag tattatcctc tgtattatgt gttataggcg ctttactcaa
     5581 aaaaaagccg actcttaaag tcggctttaa ttatttttat tctttatttc gtaccaaaga
     5641 ttttgtcacc ggcatcgccg aggcccggaa taatgtatcc gtgctcgttc agtccctggt
     5701 caatcgatgc ggtatacagt tcgacgtccg ggtgcgcttt ttccagcgca gcgatacctt
     5761 ctggcgcagc taccagcacc agaactttga tgctgctgca gcccgctttt ttcagcaggt
     5821 cgatggtcgc gataacggaa ccaccggttg ccagcattgg gtcaacgatc agcgccatac
     5881 gctcatcgat gttagaaacc agtttctgga agtacggtac cggctccagc gtttcttcat
     5941 tacggtacat accgacaacg ctgatgcgcg cgctcggaac attttccagc acaccgtcca
     6001 tcatccccag accggcacgc agaattggca caacggtaat tttcttacct ttgatctggt
     6061 cgatttctac cgggccgttc cagccttcga tagttacttt ttccgtttcg aggtcggcgg
     6121 tcgcttcgta agtcagcagg ctacccactt cggaagcgag ttcgcgaaag cgcttggtgc
     6181 tgatatcttg ctcacgcatc agtcccagct tgtgtttgac gagtgggtgt ttgacttcca
     6241 cgatcttcat actctttctc ctttgagggg cagccacaaa aaaaatcgac ggattatacc
     6301 tcctttcttc aaggcggcaa tattcttttc gttgacttta gtcaaaatga taacggtttg
     6361 agataaagtt attttatatt caaatggtta tgaaagaaga ttattccatc cgaaaactaa
     6421 cctttgccct ggcacaagtc ttctttcgcc gcgcgtctgg ggaaaagacg tgcaaaaagg
     6481 ttgtgcaaag cagtctcgaa aacgtttgct ttccctgtta gaattgcgcc gaattttatt
     6541 tttctaccgc aagtaacgcg tggggaccca agcagtgacc gataaaacct ctcttagcta
     6601 caaagatgcc ggtgttgata ttgacgcggg taatgctctg gttggaagaa tcaaaggcgt
     6661 agtgaagaaa acgcgtcgtc cggaagtgat gggcggtctg ggcggcttcg gtgcgctgtg
     6721 tgcattgccg caaaaatatc gtgaacccgt gctggtttcc ggcactgacg gcgtaggtac
     6781 caagctgcgt ctggcaatgg acttaaaacg tcacgacacc attggtattg atctggtcgc
     6841 catgtgcgtt aatgacctgg tggtgcaagg tgcagaaccg ctgtttttcc tcgactatta
     6901 cgcaaccgga aaactggatg ttgataccgc ttcagcggtg atcagcggta tcgcggaagg
     6961 atgtctgcaa tcgggctgtt cactggtggg tggcgaaacg gcagaaatgc cggggatgta
     7021 tcacggtgag gattacgatg tcgcgggttt ctgcgttggc gtggtagaaa aatcagaaat
     7081 catcgacggc tctaaagtca gcgacggcga tgtgctgatt gcactcggtt ccagcggtcc
     7141 gcactcgaac ggctattcgc tggtgcgcaa aattcttgaa gtcagcggtt gtgatccgca
     7201 aaccaccgaa cttgatggta agccattagc cgatcatctg ctggcaccga cccgcattta
     7261 cgtgaagtca gtgctggagt tgattgaaaa ggtcgatgtg catgccattg cgcacctgac
     7321 cggcggcggc ttctgggaaa acattccgcg cgtattgcca gataatactc aggcagtgat
     7381 tgatgaatct tcctggcagt ggccggaagt gttcaactgg ctgcaaacgg ctggtaacgt
     7441 tgagcgccat gaaatgtatc gcaccttcaa ctgcggcgtc gggatgatta tcgccctgcc
     7501 tgctccggaa gtggacaaag ccctcgccct gctaaatgcc aacggtgaaa acgcgtggaa
     7561 aatcggtatc atcaaagcct ctgattccga acaacgcgtg gttatcgaat aatgaatatt
     7621 gtggtgctta tttccggcaa cggaagtaat ttacaggcaa ttattgacgc ctgtaaaacc
     7681 aacaaaatta aaggcaccat acgggcagtt ttcagcaata aggccgacgc gttcggcctt
     7741 gaacgcgccc gccaggcggg tattgcaacg catacgctca tcgccagcgc gtttgacagt
     7801 cgtgaagcct atgaccggga gttgattcat gaaatcgaca tgtacgcacc cgatgtggtc
     7861 gtgctggctg gttttatgcg cattctcagc ccggcgtttg tctcccacta tgccgggcgt
     7921 ttgctgaaca ttcacccttc tctgctgccg aaatatcccg gattacacac ccatcgtcag
     7981 gcgctggaaa atggtgatga agagcacggt acatcggtgc atttcgtcac cgatgaactg
     8041 gacggtggcc cggttatttt acaggcgaaa gtcccggtat ttgctggtga tacggaagat
     8101 gacatcaccg cccgcgtgca aacccaggaa cacgctattt atccactggt gattagctgg
     8161 tttgccgatg gtcgtctgaa aatgcacgaa aacgccgcgt ggctggatgg tcaacgtctg
     8221 ccgccgcagg gctacgctgc cgacgagtaa tgccccgtag ttaaagcgcc agctctgccg
     8281 ctggcgtttt tcaattcacc tgtaaatcgc aagctccagc agtttttttc ccccttttct
     8341 ggcatagttg gacatctgcc aatattgctc gccataatat ccaggcagtg tcccgtgaat
     8401 aaaacggagt aaaagtggta atgggtcagg aaaagctata catcgaaaaa gagctcagtt
     8461 ggttatcgtt caatgaacgc gtgcttcagg aagcggcgga caaatctaac ccgctgattg
     8521 aaaggatgcg tttcctgggg atctattcca ataaccttga tgagttctat aaagtccgct
     8581 tcgctgaact gaagcgacgc atcattatta gcgaagaaca aggctccaac tctcattccc
     8641 gccatttact gggcaaaatt cagtcccggg tgctgaaagc cgatcaggaa ttcgacggcc
     8701 tctacaacga gctgctgctg gagatggcgc gtaaccagat cttcctgatt aatgaacgcc
     8761 agctctccgt caatcaacaa aactggctgc gtcattattt taagcagtat ctgcgtcagc
     8821 acattacgcc gattttaatc aatcctgaca ctgacttagt gcagttcctg aaagatgatt
     8881 acacctatct ggcggtggaa attatccgtg gcgataccat ccgttacgcg ctgctggaga
     8941 tcccatcaga taaagtgccg cgctttgtga atttaccgcc agaagcaccg cgtcgacgca
     9001 agccgatgat tcttctggat aacattctgc gttactgcct tgatgatatt ttcaaaggct
     9061 tctttgatta tgacgcgctg aatgcctatt caatgaagat gacccgcgat gccgaatacg
     9121 atttagtgca tgagatggaa gccagcctga tggagttgat gtcttccagt ctcaagcagc
     9181 gtttaactgc tgagccggtg cgttttgttt atcagcgcga tatgcccaat gcgctggttg
     9241 aagtgttacg cgaaaaactg actatttccc gctacgactc catcgtcccc ggcggtcgtt
     9301 atcataattt taaagacttt attaatttcc ccaatgtcgg caaagccaat ctggtgaaca
     9361 aaccactgcc gcgtttacgc catatttggt ttgataaagc ccagttccgc aatggttttg
     9421 atgccattcg cgaacgcgat gtgttgctct attatcctta tcacaccttt gagcatgtgc
     9481 tggaactgct gcgtcaggct tcgttcgacc cgagcgtact ggcgattaaa attaacattt
     9541 accgtgtggc aaaagattca cgtatcatcg actcgatgat ccacgctgcg cacaacggca
     9601 agaaagtgac cgtggtggtt gagttacagg cgcgtttcga cgaagaagcc aacattcact
     9661 gggcgaagcg cctgaccgaa gcaggcgtgc acgttatctt ctctgcgccg gggctgaaaa
     9721 ttcacgccaa actgttcctg atttcacgta aagaaaacgg tgaagtggtc cgttacgcac
     9781 acatcgggac cgggaacttt aacgaaaaaa ccgcgcgtct ttatactgac tattcgttgc
     9841 tgaccgcaga tgcgcgtatc accaacgaag tacggcgggt atttaacttt attgaaaacc
     9901 cataccgccc ggtgacattt gattatttaa tggtgtcacc gcaaaactct cgccgtctgt
     9961 tatatgaaat ggtagaccgc gaaatcgcca acgcgcagca agggctgccc agtggtatca
    10021 ccctgaagct aaataacctt gtcgataaag gcctggttga tcgtctgtat gcggcctcca
    10081 gctccggcgt accggttaat ctgctggttc gcggaatgtg ttcgctgatc cccaatctgg
    10141 aaggcattag cgacaacatt cgtgccatca gtattgttga ccgttacctt gaacatgacc
    10201 gggtttatat ttttgaaaat ggcggcgata aaaaggtcta cctttcttcc gccgactgga
    10261 tgacgcgcaa tattgattat cgtattgaag tggcgacacc gctgctcgat ccgcgcctga
    10321 agcagcgggt gctggacatc atcgacatat tgttcagcga tacggtcaaa gctcgttata
    10381 tcgataaaga actcagtaat cgctacgttc cccgcggcaa tcgccgcaaa gtacgggcgc
    10441 agttggcgat ttatgactac atcaaatcac tcgaacaacc tgaataaccc tatgccaata
    10501 cacgataaat cccctcgtcc gcaggagttt gctgcggtcg atcttggttc aaacagtttt
    10561 cacatggtca tagcccgtgt ggtagatggt gccatgcaga ttattggccg cctgaaacag
    10621 cgggtgcatc tggcggacgg cctggggcca gataatatgt tgagtgaaga ggcaatgacg
    10681 cgcggtttga actgtctgtc gctgtttgcc gaacggctac aagggttttc tcctgccagc
    10741 gtctgtatag tcggcaccca tacgctgcgt caggcgctga acgccactga ctttctgaaa
    10801 cgcgcggaaa aggtcattcc ctacccgatt gaaattattt ccggtaatga agaagcccgt
    10861 ctgattttta tgggcgtgga acatacccaa ccggagaaag gtcgcaaact ggttattgat
    10921 attggcggcg gatctacgga actggtgatt ggtgaaaatt tcgaaccgat tctcgttgaa
    10981 agccgccgga tgggttgtgt cagctttgcc cagctttact tccctggcgg ggtcatcaat
    11041 aaagagaatt ttcagcgcgc tcgtatggcg gcagcacaaa aactggaaac tttaacctgg
    11101 caattccgta ttcagggctg gaacgtggca atgggcgctt ccggtaccat aaaagccgcc
    11161 catgaagtgt taatggaaat gggcgagaaa gacgggataa ttaccccgga acgtctggaa
    11221 aaactggtaa aagaagtttt acgtcaccgt aatttcgcat cgctgagttt accgggtctt
    11281 tccgaagagc ggaaaacagt cttcgttcct ggactggcga ttttatgcgg tgtgtttgat
    11341 gctttagcca tccgtgaact gcgcctttct gacggggcgt tacgcgaagg cgtactgtac
    11401 gaaatggaag gacgtttccg tcatcaggat gtgcgtagtc gcaccgccag cagcctcgcc
    11461 aaccagtatc acatcgacag cgaacaggcc cgacgggtgc tggataccac tatgcaaatg
    11521 tacgaacagt ggcgggaaca gcaaccgaag ctggcgcatc cgcaactgga ggcgctactg
    11581 cgatgggccg ccatgctgca tgaggtcggg ttgaatatca accacagcgg tttgcatcgc
    11641 cactccgctt atattctgca aaacagtgac ttgccgggtt ttaatcagga acagcagctg
    11701 atgatggcga cactggtgcg ctatcaccgt aaagcgatta aactcgacga tctgccgcgc
    11761 tttaccttgt ttaagaagaa acagttcctg ccactgattc agctattgcg ccttggcgta
    11821 ttactcaaca atcaacgtca ggcaaccacc acaccgccaa cattgacact gattactgat
    11881 gacagtcact ggacactgcg tttcccgcat gactggttta gtcagaatgc gttggtactg
    11941 cttgatctgg aaaaggagca agaatactgg gaaggcgtgg ctggctggcg gttgaaaatt
    12001 gaagaagaaa gtacacctga aatcgccgct taatgctttg cgggccgacg agaaatgtcg
    12061 gcccgcatta ttcaggcact ttcgcgaatg ggttcgattt cattcaacgt atcaattaac
    12121 ggctgcggct taccaataag ataaccctgc atataatcga tccccaaaga gagcaccgcc
    12181 tcgcggatct cttcgttttc aacgtactct gccactaccc gcattttctt cattcgcgcc
    12241 agatggcaaa tcgacgccac aatctgataa tccagactat ttgacacaat attgcggata
    12301 aagctgccgt caattttaag cagatcggca ttcacatttt taagccgtgc atagctggcg
    12361 tagccggtgc cgaaatcatc aatcgcaatc tggcagccta attcctgaag atgctgcaag
    12421 gtgatttgcg cctgcttaac attggtcaga gcattacttt cggtgacttc aaaaataagt
    12481 tgccacgctt caatctgata tttagccagc aactgactga cttcaacagg aaaacgagcc
    12541 tgacataccg aggttggaga cagattaata gcaaaacggt gagcgggcat cttcgctctg
    12601 ttttcagcca taaattgcag cgtatgctca atgacccaca tgtcgatact cgacgataaa
    12661 ccaaattcgt gcgcgaccgg caagaagcta tcggggacga tcagttcatc attctcacct
    12721 ttcatgcgca gaagaatttc atggtaaaca tcaccacgca taccggtaat cggctgggcc
    12781 atcaggaaaa aatggttgtg ttccagcgcc tgctgtagtc gattcatcat cgcgacttta
    12841 tctttcaatt cgcgttgcaa atacattgcc ccgcgacgct gcatattttc cggggcgttg
    12901 gtcacgatgg aaagttcggc gaccgtattt agctctccca gcagcaggta gatatgattc
    12961 actggcgagc gcacatagca gtaactgacg ccaatctgcg gttgcatcgg catcccatcc
    13021 caaaagaaac gaaattgctt gagatgacta tccagtgcgg taatgcgctc ctggtgcgat
    13081 tctgtattca gtcgcagcgc gagatcgtta cccgaaagct gataaacatc ttcacccggt
    13141 tccagcaagg gtgacagcca gtgagaaagt ttttgcttgt attgaatccg cagcatgatg
    13201 ccatagttct taaccagcat ttccatgcca gggatgcgta aataacaaag cgcagaccag
    13261 ggagcatcac gcaacgcgcg attcagggcg cgaacatttg gcagatgaac caccgggtcc
    13321 atatacgcaa gccgctgaat gcgtctgact accgctctct gacgggttgc cagtacagcc
    13381 atataattga caataaaaga gaataccaga taactggagg aggttatggt cagctgcgtg
    13441 gtatagccag gataaatggg aatgtaattt tgatagctgt ggatgctgat catcaacacg
    13501 accgcccaga gcagcgaaat cagcttataa ccatagcgca tcgctcccca catcatcagg
    13561 ggcagcaata atgacaaggt ataattggtg ctaaaaattg tgcttttttc atttaacggc
    13621 atgcataaca gcaacaataa agcacctaat gccagtagcc agagcgcgaa ctcttttttg
    13681 gtgactttgg catcaacctg ctgttttaat tgcgaataat agctacgtaa ataaaatgga
    13741 tttcgcacta cccgaatgat gaagtagcac agcgggacac cgatcagatt acccaccagt
    13801 aaggcctgat aattgattaa agtcccgagg ttaaaaggca tgacaccgac cagattttct
    13861 ctgctcgcca gtaatcctac aaatgcagca aactggaaaa gtatcagaaa cagcgttgca
    13921 ggaaacacaa tctgccagaa tatacgctgg gaaattaaac gggtatcacc atgtgaaacg
    13981 ttgttacgcc ggggagtaaa gaccctgtaa ccgccccaac agagaatgat aatgataata
    14041 aaattagccg ttaatgatag cgtttcgtaa aaacccagct ctgcatattt gcggacaaaa
    14101 atccctaagg cgatccctgg caatgccgcc cagctgaaaa acatcatcat gctgatcatc
    14161 agtgccagag gcagataaaa gagaaaaacc tcaccggaag aaatatgcgc gaaagtatta
    14221 atgtgggcga aaatgggcaa gattaaagaa ggcaggaaca gcggaagccc ccaccattta
    14281 tcacgcattt ttatataagt tgcattcagt ttcatagatg ctcagcagaa tcccccacat
    14341 cctgaaggag gtgtattcag acaggcatcc cacctgactt cgaatgatga ttattcatca
    14401 ctatagagag cattgattct aagtgtcata tgaaagttcc aattgatata tatcaaacaa
    14461 aataaccctg attaatgaat tattacgttt atcatgttaa ttcatcatta ttacatcatc
    14521 attgtaaata attaaattaa cttccataac attaaaatat gtatccactg acgctttttt
    14581 acataacgaa gaattgacca ttttgtcctg ttgtgcctta atgtaagtac cgtccacagc
    14641 gtgggacata cttcaaggaa ccttttgtga gtcaggcaac cagtatgcga aaacgacacc
    14701 gatttaacag tcgcatgacc cgtatcgtac tgctcatcag ctttatcttc ttctttggcc
    14761 gttttatcta ctcgtccgtc ggtgcctggc agcaccatca gagcaaaaaa gaagctcagc
    14821 aatccacact ctccgtcgaa tcaccggtac aacgttagcg gttaccttct tcactttcac
    14881 agaacataac ggcacttcgc tgtcggatgc ttttgctctt tgggattatc aaagcggcag
    14941 atattctttc atcttaaatt ttacgtcttt gtcctgactg atgtttatcc tgtttggctg
    15001 cggaataaat ataaaattaa tatatatgtt gtaatgatat atttttataa attattccct
    15061 gcgtgaattt taataaattt aatctatccc tttatacgca atacatttac tttcctcttt
    15121 tgatgatctt aaatgtctta tttttcgtaa tgtgtataac aaggaatagt gatgaaattt
    15181 aaaaaatgtc ttctgcctgt ggcaatgtta gcgtcattca ctctggcagg atgccagtca
    15241 aatgctgacg atcatgccgc cgatgtttat caaaccgatc aactgaatac caaacaagaa
    15301 actaaaaccg ttaatattat ttccattctt cccgcaaaag ttgccgtaga caactcccaa
    15361 aataaacgga acgcacaagc cttcggcgcg cttattggcg cagtcgctgg cggtgttatc
    15421 ggccacaacg tcggttctgg cagcaattcc ggaacgacgg caggggcagt tggcggcgga
    15481 gctgtaggcg cggcagcggg ttctatggtg aatgataaaa ccttagtgga aggtgtttct
    15541 ttaacatata aggaaggcac caaagtgtat acctccaccc aggtgggtaa agagtgccag
    15601 tttacgacag gtttagccgt tgttattacc acaacgtata acgaaacgcg tattcagcca
    15661 aatactaaat gtcctgaaaa gagctaataa tcaggaggag tcatgaagaa agtttttctt
    15721 tgcgccatct tagcctcctt aagctatccg gctatcgcct catcattgca ggatcaactc
    15781 tctgctgtcg cagaagcgga acagcaaggt aaaaatgaag agcaaaggca gcatgacgaa
    15841 tgggtcgcgg agcgcaacag ggaaatccag caagagaagc aacgtcgcgc aaatgcccag
    15901 gccgccgcta acaaaagagc ggcaacggca gcggcaaata agaaagctcg tcaggataaa
    15961 ctggacgccg aagccactgc ggacaaaaaa cgcgatcaaa gttatgaaga tgagctacgc
    16021 agcttagaga ttcagaaaca aaaactgacg ctggcgaaag aagaagcccg cgttaagcga
    16081 gaaaacgaat ttatcgatca ggaactgaag cacaaagctg cgcaaaccga tgtggtgcaa
    16141 tctgaagctg acgccaacag aaatatgact gaaggcggtc gcgatctgat gaaaagcgtg
    16201 ggcaaagcag aagagaacaa atcggacagc tggtttaatt aatcgatgtt agtaacttca
    16261 agcctatgat tcttgaagat aaaaaaccct ctgtagtaac agagggtttt gttcattcat
    16321 agtgcaggga ttaaaatcat tcccactcaa tggtagctgg cggcttgccg ctgatgtcat
    16381 acaccacgcg ggaaataccg ttcacttcat tgataatgcg gttggaaacg cgaccgagga
    16441 aatcgtacgg cagatgcgcc cagtgtgcgg tcataaagtc gatggtttcg acagcacgca
    16501 gagagacaac ccagtcatac ttacgaccat cgcccattac gccaacggaa cgtaccggca
    16561 ggaacacagt gaacgcctgg ctgactttgt cgtacaggtc cgctttacgc agttcttcaa
    16621 tgaagatggc gtcagcacgg cgcagcaggt cacagtactc tttcttcact tcacccagca
    16681 cacgaacgcc aaggcctggt cccgggaatg ggtgacggta caacatgtcg tacggcaggc
    16741 ccagctccag accaatctta cgcacttcgt ctttgaacag ctctttcagc ggttcaacca
    16801 ggcccatctt catctctttc ggcaggccgc ccacgttgtg gtgagatttg atgacgtgtg
    16861 ctttaccggt tgcagacgcc gcagattcga taacgtcagg gtagatggtg ccctgcgcca
    16921 gccacttcac gtcttccagt ttcagcgctt cttcatcgaa tacttcaacg aaaacgcgac
    16981 cgatgatttt acgttttgct tccggatcgt tttcgccagc cagcgctgac aggaagcgat
    17041 cttctgccgg aacgtgaaca atgttcagac caaagtgatc gccaaacata tccagaacct
    17101 gctctgcttc gttgagacgc agcaggccgt tgtcgacgaa tacgcaagtc aggtttttac
    17161 cgatagcgcg gtgcagtagc attgcggtta cggaggaatc cacgccacca gagaggccga
    17221 ggatgacttt atcgtcgcct acctgctcac ggatgcgggc tacagcatcg tcgataattt
    17281 tcgctggcgt ccacagggct tcacactggc agatatcacg cacaaaacgc tccagcatgc
    17341 gcataccctg gcgggtgtga gtcacttccg ggtggaactg tacgccatag aagcgttttt
    17401 cttcgttagc cataatggca aacgggcagc tttcggtgct ggctacggtg acgaagtcgg
    17461 acgggatagc ggtaacttta tcgccgtggc tcatccagac atcgagcagc ggtttaccgt
    17521 ctgcggtcag cgcatcttcg ataccgcgaa ccagtgcact gtcgtttacg acttcaacct
    17581 gcgcgtagcc aaattcacgt tcgttagagg cttcaacgtg accgcccaac tgcattgcca
    17641 tggtctgcat gccatagcaa acgccgaata ccggtacgcc tgcttcaaag acatactgcg
    17701 gcgcacgcgg gctgttttct tcagtagtac tttccgggcc gccggaaaga ataatgccgc
    17761 ttggattgaa gtcacgaatt tgtgcttctg tcacatccca cgcccacagt tcgcagtaaa
    17821 cacccagctc acgcacgcgg cgcgcaacca gttgagtgta ctgagaaccg aagtccagaa
    17881 tgaggatgcg atgcttatga atgttttccg tcattgacgc ttattccgag gcaagtgaaa
    17941 cagataatat aaatcgcccg acatgaagtc gggcgaagag aatcaggagc ccagacggta
    18001 gttcggggac tctttagtaa tggtcacgtc gtgaacgtgg ctttcctgaa tgcccgcacc
    18061 gctgatacgt acaaactccg ctttagtacg cagttcgtcg atagtaccac agccggtcag
    18121 acccatacag gagcgcaggc cgcccatctg ctggtgaatg atctctttca ggcgaccttt
    18181 ataagctacg cgaccttcga taccttccgg caccagtttg tcggcagcgt tatcgctctg
    18241 gaaataacgg tcagaggaac ctttggacat cgcgcccagg gaacccatac cacggtaaga
    18301 tttgtaagaa cggccctggt agagttcgat ttcacccgga gattcttcag tacccgccag
    18361 catggaacct accatcaccg cgcttgcgcc agcggcgata gctttggcga tgtcgccgga
    18421 gaagcgaata ccaccatctg cgataaccgg aataccggtg ccttccaggg cttcaactgc
    18481 gtcagcaacg gcagtgatct gcggaacacc tacgccagta acgatacgag tcgtacagat
    18541 agaaccaggg ccgataccga ctttaaccgc gctgcaacca gcttctgcca aagcgcgtgc
    18601 acctgcagct gttgccacgt tgccgccgat gatttgcaga tccggatatt tagcacgggt
    18661 ttcacggata cgctgcaaaa cgccttctga gtgaccgtgg gaggagtcga tcagcagaac
    18721 gtcaacgcca gcggcaacca gcgcgtcaac acgctcttcg ttacccgcac ctgcgccaac
    18781 tgcagcacca acacgcagac ggccttgctc gtctttacag gcgttcggtt tacgttccgc
    18841 tttctggaag tctttcacgg tgatcatgcc gatcaggtgg aattcgtcat caaccaccag
    18901 cgctttttca acgcgttttt cgtgcatttt tgccagcacc acttcacggg cttcaccttc
    18961 acgcacggtg accagacgct ctttcggcgt catgtaaacg ctaaccggct ggttcaggtc
    19021 ggtaacaaaa cgcacgtcac gaccggtgat aatacccacc agttcgtttt cttcggtaac
    19081 gaccggatag cccgcaaaac cgttacgctc ggtcagttct ttcacttcgc gcagcgtcgt
    19141 ggttggcaga acagtctgcg gatcagtcac cacaccagat tcgtgttttt tcacacggcg
    19201 aacttcttct gcctggcgtt caatggacat gtttttgtgg ataaagccga taccgccttc
    19261 ctgagccaga gcaatagcca ggcgcgcttc cgttacggta tccattgctg cggaaagcat
    19321 agggatattc agacgaatag ttttcgtcag ctgggtgctg aggtcagcag tattcggcag
    19381 aacggtagaa tgagcaggaa cgaggagaac gtcgtcaaac gtcagagctt ctttagcgat
    19441 acgtagcatg ggcaatatct cgaccagagt ggttaataaa tattgccgcg gcattataca
    19501 gagcgtaacc gattgcatct accccttttt gcaaaaaatg cttgctatcc ccgaagggcg
    19561 ggttactatc gactgaataa cctgctgatt tagaatttga tctcgctcac atgttacctt
    19621 ctcaatcccc tgcaattttt accgttagtc gcctgaatca aacggttcgt ctgctgcttg
    19681 agcatgagat gggacaggtt tggatcagcg gcgaaatctc taatttcaca caaccggctt
    19741 ccggtcactg gtactttaca ctcaaagacg acaccgccca ggtacgctgc gcgatgttcc
    19801 gcaacagcaa ccgccgggtg accttccgtc cacagcatgg acaacaagtt ttagttcgcg
    19861 ccaatattac gctctacgag ccgcgcggtg actaccagat aatcgttgag agtatgcagc
    19921 cggccggtga agggctgctg caacagaagt acgaacagct caaagcgaag ttgcaggctg
    19981 aaggtttgtt cgatcagcaa tacaaaaaac cacttccctc ccctgcgcat tgcgttggtg
    20041 tgatcacctc aaaaaccggt gctgcgctac atgatatttt gcatgtgtta aaacgtcgcg
    20101 atccttctct accggtgatc atctacccca ccgccgttca gggcgatgac gcaccggggc
    20161 aaattgttcg cgccattgaa ctggcgaatc agcgcaatga gtgcgacgtg ttgatcgttg
    20221 ggcgcggcgg cggttcgctg gaagatttat ggagttttaa cgacgaacgc gtagcgcggg
    20281 cgatttttgc cagccgcatt ccggtcgtca gcgccgtcgg gcatgagacg gatgtgacca
    20341 ttgccgattt tgttgccgat ctgcgtgcgc caacgccgtc tgccgccgct gaagtagtga
    20401 gctgtaatca gcaagagtta ctgcgccagg tgcaatcgac ccgtcaacgg ctggagatgg
    20461 cgatggatta ttatctcgcc aaccgcacgc gtcgctttac gcagatccat caccgattac
    20521 agcaacagca tccgcagctc cggctggcac gccagcaaac catgcttgag cgcctgcaaa
    20581 agcggatgag ctttgcgctg gaaaatcaac ttaagcgtac cgggcaacag cagcagcggt
    20641 taacacagcg gctgaatcag caaaatccac agccgaagat tcatcgcgcg caaacgcgca
    20701 ttcagcaact ggaatatcgt ttagcagaaa ccctgcgcgt acagcttagc gccacgcgtg
    20761 aacgtttcgg taatgcagta acgcacctcg aagccgtaag cccactgtca acgctggcgc
    20821 gtggatacag cgttactact gctactgacg gcaatgtact gaaaaaagtg aagcaagtta
    20881 aagcgggtga aatgctaacc acacgtctgg aagacggctg gatagaaagt gaagttaaaa
    20941 acatccagcc agtaaaaaaa tcgcgtaaaa aggtgcatta agccagcacg aattcaacgc
    21001 gttttttcga tatcaagcca tgcccgtgct gacagaagta atctactgca ccgcaggctt
    21061 ttaacacctg caacggttga tggcagtctg gacaaagagc tttcatttca ataatttccc
    21121 cgcaggatgg gcaacgcgca tggccgttat cctgatcaag cacatgctga cattgtggac
    21181 aatgcagttc catatgaatt cctccggtag ccatccctct acattcatag agggaatggc
    21241 agataaaata cttacggata attatttatt tttcttgatg tgcttcatca gacgcttacg
    21301 tttacgcatc tgggttggcg tcagggtgtt acgcttgttc gcatacgggt tttccccttc
    21361 tttgaactga atacgaatcg gcgatcccat tacgtccagc gatttgcgga agtagttcat
    21421 caagtagcgc ttgtaggaat caggcaggtc tttcacctga ttaccgtgaa tcaccacaat
    21481 cggcgggtta taaccaccag cgtgggcata tttcagcttc acacgacgac cgcgtaccag
    21541 cggcggttgg tgatcttcaa cagccatcgt catgatgcgc gtcagcatag aggtccccac
    21601 acgacgggtg gaactgtcat acgcttcacg tactgattca aacaagttac caacaccact
    21661 gccgtgcaag gcagagataa agtgcacacg agcaaaatcg ataaagccca gacggaagtc
    21721 cagcgtttct ttcacctgct ctttcacttc ctggctcagg ccatcccact tattcaccac
    21781 aatgacaagt gagcgcccac tattgagaat aaagcccagc agcgagagat cctgatcgga
    21841 aataccttcg cgcgcatcaa tcactaacat caccacgttg gtatcttcaa tggcctgcaa
    21901 cgttttgatt acggagaatt tctctacagc atcggtgatt ttgccgcgtt tacgtacgcc
    21961 agcggtgtca atgagcacat actcacgtcc atcgcgttcc attggaatgt agatactgtc
    22021 acgcgtcgtg ccaggcatgt cataaacaac aacgcgctct tcaccaagaa tacggttagt
    22081 gagtgtagac ttacctacgt tcggacgacc cacaatcgcc agtttgatcg gcagactttg
    22141 cgggtcgaag tcgtcttcct cttcttcttc gccgttctct tccgcttcaa attgcgccca
    22201 gtattcagcg tcttcgtcga cttcctcttg cggtgcgaga tcttccatcc acggcagcag
    22261 cacacgctcc agcagactta atacgccacg accgtgagat gcggcgatcg ggtagatttc
    22321 acctaaacca agcgagtaga aatcaaccac tgcctgatcg ggatccagac cgtcagtttt
    22381 gtttgccacc aggaaggtcg gtttttcacg agagcgcaga tgtttggcaa tcgcttcatc
    22441 tgccggcatc aagcccgcgc gcgcatccac cataaacagt acgacgtccg cttcttcaat
    22501 cgccagcagc gactgttccg ccatgcgggt ttctacaccg tcttctgtgc catcaatccc
    22561 gccggtatca atacagataa actcacggcc ttcaatttcc gcacgaccgt acttacggtc
    22621 acgagtcaga cccgggaaat ccgcaaccag cgcatctcgg gtgcgagtta gacggttaaa
    22681 taacgtggat tttcctacgt tagggcgccc gacaagcgcg accacaggta ccatgtttaa
    22741 agcctcattt ttataaatca tcagacaacg cacgctatat tcgcgtcgtt gttaaaaaca
    22801 ggaaaacggc ccctgtccag gagccgtttt caaagtgaac gacagagacg attaacgtgt
    22861 aatagagtac acggttccgt cttttgcctg gatcagcagt ttgccgtcag cggcaaccgg
    22921 ttcagtctgg aaaccggaac tatcaacttt ttgctgggca acgaaacgac catcttcgac
    22981 gttaatccag tgcagataac cttcactgtc acctaccacc aggttgccat tatacagcac
    23041 cggcgaagtc agcaggcgat gcagcagatc gctttgtgtc cacagcgtaa cgccgccatc
    23101 aatggtcaac gccatcaccc ggtcattttg atcgaccaga tagatgcgat tgccgtcgac
    23161 gatgaaatca ttcaccgaac ccagttcgcg tttccacata atctgaccac tgcgcagatc
    23221 aagcgccgtc aggttaccat tataggccag agcgaaaaca acgccgttaa caacgacggg
    23281 agtcgtgtca acatcgctca gacggtcaat ttcggtagaa ccggtcgcct gggaaatacg
    23341 ctgctgccaa atcatctggc cctgttccat cagcactgcg ctgacgcgac cattatcgcc
    23401 ccccacgacg gccgcaccaa aagccgttgc cggcgcagac tcgccacgca aagagagcga
    23461 aggcatatcg aggttaactg tccatttgac agcgccgtca gcttcgttca gcgcttgtaa
    23521 ctgaccgtta ctagtgtgga ttaacaccag accgtcgctg accaccgggc gagaaagtgc
    23581 ttcacccgcg actttagttt gccatgccac agtaccatcg ctggtattca gcgcgtaaac
    23641 ctgcgccttt tcgctgccaa tgtagacatg cccaccagac acggtcacac cgccagaaag
    23701 taatgcaggc tctttagaga accagccatc tttctcggcc aggctgacag accagatttc
    23761 tttgccatca tccgcattca gcgcttttac caaaccagcg cggtccgctg catagacaac
    23821 gttgtccgcc agtgccggat gaagattgga atagaagttg ccaatgccgc taccaacgga
    23881 agtgctccac gccgtggtcg gcgtaaactg gttttcaacg gttggcaatg gggacatctt
    23941 taccacatct tcttcgctgt taaacagcga acagccgctt aaaagggtaa cggaaagcag
    24001 tcctggcagc agtaatttac gcaattgcat cgggtccctc tcagatggac aaattattaa
    24061 ttttcatctg catcatttcg ctcagtgccg gagtaacatc gcttttcacg cctgcttccc
    24121 atgcactacg cgcaccttgc ttatcacctt tgctcaacaa tgctttacca cgcaggtcgg
    24181 caacaatggc agcccaccct tcacctttga tggtatcaag ggttttcagc gcggcatcag
    24241 cctgcttgag ctgtacctga acgcgagcaa gacgcagatt tatcacggct ttgagatttt
    24301 catcactcgt gtctgccagc ccctgttgta actgggcggc agctttctct agttcatttt
    24361 tgtcaacaaa ttgctgcgca agttccaaag aagccagcgc accataagta tttttatttt
    24421 cagcagcaaa tttttccgcc gccgggatgc tatccggttt gccttcgctc actgcggtaa
    24481 ccgcattttg ataggcaaga gaagcggagc gtgcagaatc aacctgatgg ctgttccagt
    24541 agcgccagcc aatcagtgcg ccaacgccca aaatcacccc aacagccagt gctttgccat
    24601 tttcagcaaa aaagcgttta accgcttcta cctggtcgtt ttcgttctcg taaatttcca
    24661 cgctgtcctt ctccttcctt aacccagtaa cgtgcgcaaa tgcgcggcta cgctatcctg
    24721 cgcaactgcc gtttgctcac cagagcgcaa atccttcact actgctgtgc cgttagccac
    24781 ttcagactca cccagcacca cagcaacgcg ggcaccccat ttatcagcac gggcaaactg
    24841 tttcttaaag ttgccgccgc cgtggttggt catcaatttc acgcccggta attcatcacg
    24901 cagacgctca gctaatgcca tagccgcaga ttgtgtatca gcacctgaag ccaccaggta
    24961 tatatcgaca acaggatcgg ctttaaattc cggattaacg gcctgtacta acaatacaag
    25021 acgttcgagg cccatcgcaa aaccgactgc cggtgttgca cgaccgccca gttgttccac
    25081 cagaccgtca taacgaccgc ctgcacacac ggtgccctgg gagccgaggc tgttagtcac
    25141 ccactcgaaa acggtacggt tatagtaatc cagaccacgc accagacgct ggtttacggt
    25201 gtaagcgatc cccgcgctct caagcagttt gcacagaccg gcaaaatgct cacgagattc
    25261 ctcgtccaga taatcaccta atgccggagc gtcgttgaga agcgcctgca cttccggatt
    25321 ttttgaatcc agcacgcgca gcgggttagt gtacatgcgg cgtttgcagt cttcgtccag
    25381 cttttcttta tgctgctcaa ggaatgccac cagcgcatcg cggtaattgg cgcgtgcttc
    25441 cagcgaacca atagagttca gctcaagagt tacgtgttcg aagataccca gcgcacgcca
    25501 ccagcgggca gtgagcataa tcagttcagc gtcgatatcc ggaccttgca gaccgaaaac
    25561 ttcgcagccc aactgatgga actgacgata acgccctttc tgcggacgct cgtgacggaa
    25621 catcggcccg atataccaca gacgctgttc ctgattgtac agaagaccat gctcgatgcc
    25681 ggcgcgtaca cagcccgccg tcccttcagg gcgcagagtc aggctgtcgc cattgcgatc
    25741 ctcaaaggtg tacatctctt tttcaaccac gtcggtgact tcaccaatcg cacgtttgaa
    25801 tagcggggtc tgctctacaa tcggcaagcg gatttcactg taaccgtagc tgccgagcac
    25861 gtttttcagt gtgccttcaa tgcgctgcca gatggccgtt tcgccaggca ggtaatcgtt
    25921 catgccgcga atggcttgaa tgttttttgc cacgtttatt ctctttctga atataaaaat
    25981 gaaccctcaa cgcttccctc aatgtttcgg gagccatgcg ggttcaatca tacacgggaa
    26041 gcgaggcgct tcccatcacg ttattatttt tcaacctgct gaacgtcaat tcgacgcgct
    26101 tcgtccagct gactggcttt cgcacgaatg cgtgcttcca gctggtcgat catatcgttg
    26161 ttgtccagac ggtctttgcg cacgccatct tcatagaggc cgcttttctt gttgccgccg
    26221 gtgacgccga gtgtagaaac cagcgcctca cctgggccat tcaccacgca gccgataatc
    26281 gaaacgtcca tcggagtgat gatatcttcc aggcgttgct ccagcgcgtt aaccgtaccg
    26341 ataacatcaa attcctgacg cgaacaggtc gggcaggcga tgaagttaat ccctcgcgaa
    26401 cggatacgca gcgatttcaa aatatcgaaa ccgactttga tctcttcgac cggatcggcc
    26461 gccagcgata cgcgcagcgt gtcgccgatg ccttccgaca gcagcagacc taaaccaatg
    26521 gcggatttta ctgccccgct gcgtgcacca ccggcttcgg tgatccccag atgcagcggc
    26581 tgatcgatct gttttgccag caaacgataa gactcaacag cgaggaagac gtcagacgct
    26641 ttcacgctga ctttgaactg atcgaagttc aggcgatcga gatgatcaac atgacgcatg
    26701 gcggattcca gcaacgcctg cggcgtcggt tcgccatact tttcttgcag atctttttcc
    26761 agcgatccgg cgttaacgcc aatacggatc ggaatgtttt tatcgcgcgc acagtcaacc
    26821 accatgcgaa tacgctcttc attaccgata ttgccagggt taatacgcag acaatcgacg
    26881 ccgtattccg ctactttcag cgcaatgcga tagtcgaagt ggatgtcagc caccagcggc
    26941 acgttaaccc gctgtttgat gagtttgaac gcttctgccg cgtccatcgt cggtacggag
    27001 acacggacga tatcagcgcc aacgcgttcc agcgccttga tttgattgac cgttgcttcg
    27061 acatctgtcg tacgcgtatt ggtcatggac tgtacggcga tgggagcacc atcgccaatc
    27121 ggcacattcc caacgtaaat acgtgttgat tttctacgtt gaattggagc ctggttatgc
    27181 atgaaaaatc tcccgcgtta cccgtctgtt actgcgccgg tgattgttcg gcattgaggg
    27241 tcagacgcgc aacctggtta gttctgataa aacgactcag atcgacaggt ttcccttgat
    27301 actggatctg tactgcggct ggcgcaccaa ttttcagttt atacggtgcc tggccggtta
    27361 aattcaaatt accgtcttta cgctgcatcc cgctaaacaa ttttttaccg gtagcatcag
    27421 tgacctccag ccagcaatcg gcagtaaagt tcatcaccag cgcattcgga tcagccgccg
    27481 gcgtggtcac gccagcctga tcggttggca acggcgcagc accatctggc gttgttgctg
    27541 ccgttggtac cggggtcgcg gcggtatcaa catttgcctg cgaaggcgaa acaaccgcat
    27601 tctgttgcgg atcaacagct ggtgctggcg cagttacggc aggtgtttgc gtgttggttg
    27661 cggtagtatc cacagacgct ggcggcgtgg acgttgtagc cgggtctgta gttgtcgacg
    27721 tatttaacgg gacactctgc ccctgctcgc tattactgct cagttctgcc gaagattgat
    27781 cggccatagt ggtgatctct tcctgctgag ctttgtggtc ttgccaccac caggcaccgc
    27841 tcaggccgat aaccacaaac aacaccagcc aggtgaaggt catcagccag ccatcacgtt
    27901 ttttgcggcg tttaccgagg gaaaaactct gcattggcgc aacttttgca gcccgtagtg
    27961 gagcctgctt ttccagccct ggcagcagtt cttcttctgg aatatgtacc agacgcgcat
    28021 aagagcggat atatccgcgc aggaatgttg aagcaagatc ggcgggtgcc ttatcttctt
    28081 caatgtcgcg taccgtggaa accttcaggc aaagtcgctc ggcaacggcc tgctgactaa
    28141 gtcctagttg ttcgcgagca ttacgcaggc gagcgccggt agtaagtgct tcattttggt
    28201 cgtgcgtggc ttcagtattc attcgctgca ggtacgttta aatgagaatt gagatgccgg
    28261 tgaaccatca tgctcaccca caccgcgaaa catccgttaa gttaaccgtt atcatacagt
    28321 ataagaccgt ctgtgtgctc gtgacaaagc ccacattcac taaggctaac ttactgttgc
    28381 atcgttacat actgccttaa agtcagcaaa aacgcaccgt taatattgac cagacaattg
    28441 caacttaata cttcattcat aaaacattac gccacggtac ataaagtaac cgtggcgtaa
    28501 tggctatcag accgctttaa tgtcgatggc ttcaccctgc atccgtttac gcagggtacg
    28561 tttcgtacgg tcgataacat cgcccgccaa ctgaccacag gcagcatcga tatcatcacc
    28621 acgagtttta cgcacaatag tggtgaaacc gtagctcatc agcacttttg agaaacggtc
    28681 gatacggctg ttcgagctgc gtccatacgg cgcacccggg aacgggttcc acgggatcag
    28741 gttgatctta cacggcgtat ctttcagcag ttccgccagt tggtgcgcgt gttcagtgcc
    28801 gtcgttaacg tggtcaagca tcacgtattc aatagtgact cggccctgat tggcgttgga
    28861 tttctccaga taacggcgca ccgcagcaag gaacgtttcg atattgtact ttttgttgat
    28921 cggcacaatt tcgtcacgaa tttcgtcgtt cggcgcgtgc agggaaattg ccagtgcaac
    28981 gtcgatcata tcgcccagtt tatccagcgc cggaactaca ccggaagtag aaagcgtgac
    29041 gcgacgttta gacaggccaa aaccgaaatc atcaagcatg atttccatcg ccggaacgac
    29101 gttgttcagg ttgagcagcg gctcgcccat gcccatcatc actacgttag tgattggccg
    29161 ctgaccggtg acttttgctg cgccgacgat tttcgccgcc cgccacacct ggccgataat
    29221 ttccgacacc cgcaggttgc ggttaaagcc ctgctgggcg gtggaacaga atttacactc
    29281 cagcgcacac cccacctgcg aagagacgca gagcgtggca cggtcgtctt ccgggatata
    29341 caccgtttcg acgcgctgat cgccaacggc gatcgcccat ttaatggtgc cgtcggatga
    29401 acgctgttct tcaaccactt ccggtgcgcg gatctccgcc acctctttca gtttgccgcg
    29461 caacactttg ttgatgtcgg tcatctcatc aaagttgtcg cagcaatagt gatacatcca
    29521 cttcatcacc tgatcggcgc ggaagggttt ttcacctaaa tctttaaaaa actcccgcat
    29581 ctgctgacgg ttgagatcca gcaggttgat ttttccatct ttcgtggtga cgttttcagg
    29641 tgtgactaat tgttcagaca tatgctattc cggcctcgtt attacacgtt atggcccctg
    29701 gagggttgaa aaaagaaacg ccccggtgag cggctgctcg tccgggggcg ctgcattgta
    29761 caaattctgg cgcacggatg ccacgtttgc acgcggcatt tacgaaatta ttaacgggtg
    29821 cgcgggcaca cttcgccttc gccgaagaaa taagcgattt cgcgagcggc agattcgacg
    29881 gaatcagaac cgtgggtacc gttttcggtc aggctgtcag cgtaatcagc gcgcagagta
    29941 ccggccagtg cgtttgccgg attggtcgcg cccagcagat cgcggtgacg ctgaacggcg
    30001 ttttcacctt ccagcacgga aaccacgatc gggccagagg tcatgaattc aaccagacca
    30061 tcaaagaacg gttttccatc gtgttcagca taaaagccac gagcctgttc aacggtcagg
    30121 tgcagcattt tggtgccgac aattttgaac cctgcagctt caaagcgcgc aaagatatta
    30181 ccaatgacgt tttttgctac cgcgttcggt ttgatgatgg aaaaagtacg ttcaatagcc
    30241 atttttacct ctgtaaattg ttctgttgtt gtctgtacca gcgtacgaga tggcgcggat
    30301 tataatgagc aacagggccg ttgactattg atgaaggtaa cattttttta aaataaaatg
    30361 agttttagca acaatcaggg acaaaacaga ctattgcatg acaaatttca ctgtcgcgat
    30421 ttgccccacg tcatccatca ccagcaattg ataatcacct ttatccgtca aatgcagggt
    30481 cacgttgcgc ccgcgttcag ttaacggttc gccattcaga aaccaccagc gttcacctgc
    30541 ccctccactt gattgcaacg gcaaagtcgc ttccgctgcg cccggtaaac gtttaataat
    30601 cgcgccatcg cgaacgcctg tcagctgcag agggagctgg gcatcgtgac cgtatggcgg
    30661 gcaaattgtc gaggccggtg gtaagcgcac agcgcgacgt tctgatgcgg gcagccaggg
    30721 ttccagcggt agcggccaga catttaccat ttcttgcttc gcctgcgggc agtcagcggc
    30781 gacgcgctgc cctttgtcat ccagccagat ggggaagcga atgccattaa tcccttcctg
    30841 ctctgacagt aacagcgttg gcggctggct gccatccagc agccaggttg ccaggcggcg
    30901 gcgacagtta ccgtcacctt ccggcaaaga ctgtccgccc ggccagcaga taacgccacg
    30961 agtgactgag ttcgggcgcg ggtcttccgg cagattcgca ctgcgcgaca gtaagatatt
    31021 attgacctga ttcaacaatg gtacggcact ggcaaagcca aactgaccaa caacgggcgt
    31081 gccgtcaggt ctgccagtcc agatcccaat gacatagcga gcgttaaccc caatcgccca
    31141 ggcgtcacga tagccatagc tggtgcccgt tttccatgcc agtggggcga cgcgcggcaa
    31201 ggcactatcc ggcaagggtt gcgcttcatc agccataatc cggcgaatga tccacgccgc
    31261 ccccgacgac attaaaggcc gttcaagcag cggatcgtca ggctgtaagc gcaatttgcc
    31321 tgccttgccg tggcgagcaa acgcggtata cgctgccgcc atatcttcca gttttgcacc
    31381 agcgccgccg agaatgagtg aaagattcgg cgcagcgccg ttgggcaaat ataacggcaa
    31441 tccaacattg cgtaactttg ccgcaaaccg tttcggtcca taggcttcca gcacctgcac
    31501 agcaggtaag ttcagcgagc gcaccagcgc ctcgctcatg ctgatcgggc catgaaaacc
    31561 gctatcaaag ttacctggtc gataatcacc ggtgcgccgg gggacgtctt gcagcagtga
    31621 tgccgggtgg atcaagcctt catccagcgc cagaccataa acaaacggtt tgagcaccga
    31681 tcctggcgat cggatcgcat tgaccatatc aacatgacca aagcgtgaat catcgttgag
    31741 atcaaccgat cccacccagc cgcgaacacg catatcggta tgatcaacca cgatcatcgc
    31801 cagtgagctg cgcggtggca accgcccttt ccagttttgc gccagttctt ccagacgtcg
    31861 ttgaagaccg gcatccagcg tagtaacgat tttgtcgctt ttgcttttac cgagcatcat
    31921 gcgcgaaaac agcggtgcca gttgcggcat ttgtcggggt gccagccaga tgggttcttc
    31981 ccttgactct tttacctgct cacgggacca cacaccttgc gcagccatcc gttcgagcac
    32041 tttattacgc gcggcttcgg cacgctccgg ccaacgatcc gggcgaagac ggctgggcgc
    32101 ttgcggcaaa accgccagca ttgccgcctc ggaatagctt aaattcgcag gcgactttcc
    32161 gagataagcc caacttgccg caccgatccc ctgcaacgta ccgccaaacg gagcgcggtt
    32221 aagatacaag gtcagaattt cacgcttaga cagatgccat tccagttgca acgcgcgcca
    32281 gagctggcga attttgccgc caaatgtttt ggggtgagga tcaagcagac gagcaacctg
    32341 catagtgagc gtgctgccac cggaaataac ccgtcccgaa gtgagatctt gccatgctgc
    32401 gcgcgccacc gagaatggat tcacccccgg atgcttccag aaccagcgat cttcataatt
    32461 gatcagcgct tcaaggtaac gtggagaaac atcttcgatt gttaccggat aacgccagat
    32521 gccgtcagca tcggcgaagc gccagagcgg cgtaccatcc tgcgccacga ccactcgtgc
    32581 gggattgact tcatgcagcg gtagaggcca gagtttatct gctccccacg ctgcaagaaa
    32641 aaggagaaat ggcgcagctg ccagcgttat ccagcagccg cgtttggtta acaagcgagg
    32701 catttacggt cggacaatca gcagatcttc agccgcgccg gtcgcccgcc attggggaac
    32761 atacattgat tccaccatcg gttgcggtac ctgatacgtt cccggcgtca ccgcccgcgc
    32821 cagatacacc agcgttaccg gttggtattc atcaacggca accgccgcca caaagcgatc
    32881 gtcacggaac tcaatgtgct taatactcgc ctgctgcatc tggttcagta agttctgcac
    32941 ttcgccacca ctttgctcca ggctggcgct accgttcgcc agattctggt tttccagttc
    33001 cagacccaca ggcagcagat ccacgactaa cgcatccggc acgctgttac tggcttttac
    33061 ctgcaaccac accagcacca gatcgccgct acgtaacgag tccagcgatt tgctcttacc
    33121 atcagtacca agaatatgac gctcgatttg cagcacattg ttcgccggta aaggtgcgga
    33181 ttgcggataa ccgctggcat ccatacgcaa ccataacggc tgatcgccac tgttgctcac
    33241 ctgcaaggtg acaagttgat cgctattcag attgctgttt tgcgctttct cgcctgtcag
    33301 cggctcagct gagaaagagg tttgcgcctg ccatttaccc ggtaaatcct gaatcgtacg
    33361 ggcagccagg aacaacgcgt tactttcctg cgtcgatagc cagcgttcac caaacgcctg
    33421 ctgcgaaagt gtgttcagca aagtgtactg ctcatcgggt agcagtttat tttcttccag
    33481 caaggagagc attaacgcgt tgtcgcgcag tggactaccg taatcaccca gccatatccg
    33541 ctcatcacta tttctcggcg ttttcagcgc cagcgcaatc gcttcttcac cacgcgtcgc
    33601 atcacccatg gttttcagcg caatgccaag ttgcagcagc ggtaaaccag aagcggcatc
    33661 tgcacgatgc tcccagattt cacgcagcgc acccagtggt gctttttgct gacgggccag
    33721 caccagcgca gcgtaagact gaacggcgaa tttactggct ttgagattat ccgcgtacgg
    33781 gatcgacatc atgcccggat cttgtaaata gcgcagcaga cgctcattac cccggttaat
    33841 ggcgtctgtc ggcacgctgt aaccctgttc gcctgcgcgg accaggaaat ccatcacgta
    33901 agccgtcagc cagtactctt cgtcaccgtt tttatcccac agcgcaaagc cgccgttatc
    33961 acgttgcatt tgcagcaaac gggaaatgcc gatatcgacc gatgcacggc gtttctcatc
    34021 actgtcgcct ttgatgccca acgcctgcag ttgggctgcg ttggtataaa gtgacgggaa
    34081 caggccgctg gcggtttgct caagacagcc gtacggatac gcttttaact ctttgatata
    34141 acgtgcaatg ttcagcggtg gtttgccgct caacaacaat tgcccttcca gcgtaacagg
    34201 cgagaagttt tgcaatccat ccgccggaat cgcccatgtc tcaccaggct gtaacgccgt
    34261 accgtaatta accgtttgtg ccgggaacgc cggacggacg ccgattttcc actgcttatg
    34321 ctgatcggca acggtttcac ccggtaacgc taacccgcta atggtggcct gaatttctcc
    34381 atcgccataa cccggcaatg ctcgcaccgg gataaacagc gtagtacgca cacctggcgc
    34441 taattcaacg gctgcgggtg aatcgctgac cagttcaagc aaaccactgg cggtcagggc
    34501 aacgttcagt ttttgcggtt tatcggtaag attagtgata tccagcgtca gacgcgaggt
    34561 atcgccactc gccataaagc gcggcatgtt cagttcagca atcaccggtg cggcaactat
    34621 cactttgctt tcgttgctac cgaaatcatc tgccgtccag gcttgcgcca tgacgcgcaa
    34681 ttcaccgtta aaatcgccaa tcggcagtgt aaccgagcct tcgccctgtt cgttgagcgt
    34741 taccgacagc gcctgctgcg cgacaatatt gacgtgattg accggcggtt taccaccacg
    34801 tttcagctca tcaccatcgc caccgaaacg cagagctgcc agacgcccct gaccttcaat
    34861 aacctgaccg taaatatcgt aaatgtctgc gccatagcgt ttctgaccaa agaacgcctg
    34921 ccacggatct ggcgtaacgt agtcagtaat attcagcaca ccactatcaa cggcagacac
    34981 cagcacattc acctgtttag gcttctcgcc atttttagtg ctggctttaa ttttcacggt
    35041 taatggttga ttgggacgca tttttgctgg tgtttccagc gccagatcga ggcgacggtt
    35101 ttcatcacca agcggcagat gcaacacacc aaccgcgcgt tttggcgtcg cggagcgaga
    35161 tttatcgcca ggacgcacca ccagcgtact caaataaaga tcatgacgat tccaggtttt
    35221 atcgaccgga atcgtcagat ccagcccttg agccggaaca tcaatctctt gccaccacag
    35281 cggcccttca ctggactcga ccatcgcata acctttaccc gccgttggcg cggcaatatg
    35341 caacttaatg gtatcgccgg ggcgataact ggctttatct agtttcagcg tgacacggtc
    35401 gggtcgcact gcgccgctcc cgtcgctgtt gtcctgccag ctatagccag cccaaaaacg
    35461 cacgctactg accgcttcat tcggcgcttt gacttccaga cgataagcgc cccactctac
    35521 cgggaaactg accttaccgg tttcatccgc ctgaagatcc agagtttgtt cattttcgat
    35581 cagatctttt tgatcaaact gtgactgcca gccttcatct tctgaccagt tccagtagta
    35641 atcgcggcgt tcgcgaatca ggcgcacctg caagcccgac acggcttttt tcacgccttg
    35701 cgcatcgcta taaacgatgt caaaagcggc gttactgcct tcatcaacaa tgggctgttt
    35761 caccgtgctg tcagtgcgat aatcgtaaac cgatttcaag gcgaactgcg gacggagccc
    35821 cggcaatgca tcggcaggcc agatagcctg ctcagcgcgg cgcgtcaccg ggcgaccgcc
    35881 cgattccagc aaactgccct ggaaaataac ctgcaatggg gaatgcgttt ccttccactg
    35941 gctttctgta gaaacttcgc cgcgcccttt atcatccagc gtcaactgaa cttcatccag
    36001 cgtgcgggaa aggttttcgg cagctatatc gccgaattca aaaccaggta aggctgacac
    36061 agcctcacgc agtgggcgca ggaaaagttg cccttgcaaa gtattaccat tagcaggcgc
    36121 accgtacagg tagtatccca ccacggagaa tttcacttca tcattcggcg taagcggggt
    36181 tttctcaccg gtcagattca gcgccatacg ctctggcata aaatcttcga cgtggaaatc
    36241 ccacatccgg tactggttat cgcccgtgtt ggcgcgaata tgccacatac cggttgccgc
    36301 attgctatcg agtggccagg taaagtggta gaggccattc tccggctgac taacgacgtt
    36361 cctgagtacc tgcccgtcag gtttaatcac gtctaacttg atgggttgat cgggcaatgc
    36421 tttaccgtct gcatcgcgca gtaaaccatt gaggattacc gtttcacctg ggcgataaag
    36481 atcgcgtggg ccaaacatga aaaactgttt gctatagcct ggcgcgccag caatgttaaa
    36541 ttctgctaag tccagcgccg gaagttttaa atcgagtagc gttgtctgac cgtctttacg
    36601 tgccagcagt aatgccgcat ttttatcatt ttccagctgc acatgcccct gagcgtcact
    36661 ggttgcctga gtcagagtct gtcctttctc atttaataaa gagacttcaa ttccttgctg
    36721 ggccgcgccg ttttccagac tttgggtaaa gatatccaga cgattgtgat aacggtgagc
    36781 tgaaacgccg atatcactta acgtaaacag cgtcgcggga ttactgtaat cgtaacgtcc
    36841 agcctgattc atcacagcca gatacacgcc cgcctgctga agcggtttga tatcgcccag
    36901 cggcagcaat aatttttcac gggtgttacg cgcaggattg agatcaaacc gtccggtgta
    36961 gaccagatcc gccatctgca gcagtttgtc tgactgccag ttcgccagcg aattgcggta
    37021 ttcccattgg ctaatgaatg ctggcagaga ttctggctta acgcggaaga agttaacatc
    37081 gacattatta acgttgagcg ccattaccgg cagcccttca acgactttgc caggcagcag
    37141 cgaaccacgg ctggcaaaac cgacgctggg ttggatgtcg cgggtagtta tagttttttc
    37201 gtaatcttta ctgaaggttg cgttgttgag tgctttgacc tccttgccaa tagtaacgat
    37261 caaatcacgt ttcggttcga ggtggcgtaa acgtagctct ttaagattat ctgacagctc
    37321 ccaggcacca tccactttgc cgcttttttt atcgacgaca tgaataacgc gtgagaaatc
    37381 ctgatccggg tcgagaggga tggagaacgt cagcaccagc gtggctgcac catcaagttg
    37441 gacttcagag agatccagca atgtcagcac cttaccggca ctctgttggg ccagtttttg
    37501 tctctccggc acggagagtt ttgctgaact cgcgttttct gaagaggccg ctttagtaac
    37561 ttcagaaggc gcatcttttt tcaccgctgt tggcgcgtta tcgttgttgt cgcaccctgc
    37621 cagcgccagc attagcatgc aggcggctac gcgtaacttt ttcatttttc atccctgcca
    37681 caatggcccg ttagcaacgt cgaataatta ttatgcgtga gaatttacgt tatgtaattc
    37741 agtatcacgc ccatacaatc cgaattttag tgagaattgt ggctcagaca gccaaatcag
    37801 cctctttcgc ttgtcgcttt tcactaaaca tccgacaatt tagcctaacc ccggcaaaaa
    37861 tggagatgcc tatgtccacg acatggtttg taggagccga ctggctcgcc gaacatattg
    37921 atgacccgga aattcagatt atcgatgccc gcatggcgtc gcctggacag gaagatcgta
    37981 acgttgctca ggagtatctg aatggacata ttcccggcgc agtgtttttt gatatcgaag
    38041 cgctttctga tcacacttcc ccgcttccgc acatgctgcc gcgcccggaa acgttcgccg
    38101 tggcgatgcg tgaattaggc gttaaccagg ataagcacct gattgtctat gacgaaggta
    38161 atcttttctc agccccacga gcatggtgga tgctgcgcac ctttggtgta gagaaagtgt
    38221 cgattctggg gggtggactt gcaggctggc agcgcgatga tctgctgtta gaagaaggtg
    38281 cagtagagct gccggaagga gagtttaacg ccgcgtttaa tcctgaagcc gtggtgaaag
    38341 taaccgatgt attattggca agccatgaaa atacggcgca aattattgat gcccgcccgg
    38401 ctgcacgttt taacgcagaa gttgatgaac ctcgcccagg tttacgtcgc ggacatattc
    38461 ccggagcact gaatgttccg tggacggaac tggtgcgcga aggcgaacta aaaacgaccg
    38521 atgaactgga tgcgatattt tttggtcgcg gcgtcagcta cgacaaacca attatcgtca
    38581 gctgcggctc tggtgtaacg gcagccgtgg ttttgttagc actcgcgacg ctggatgtgc
    38641 caaacgtgaa actgtacgac ggcgcatgga gtgaatgggg cgcgcgggca gatttaccgg
    38701 ttgagccagt gaaataagta ttttacaggc aataaaaaac cgccgaattt ggcggttttt
    38761 tattgctggt ctggttcgcg gcctttccag caggttgtat taccgtagta atgcaagcgc
    38821 gtctcagcgg agacaatact cgccagtaac tctctttttg tcaagcaaaa gagagttatt
    38881 attgttctgt tagtgtatta tccactgcgg ccctttccgc cgtctcgcaa gcgggcgctg
    38941 gcttaaggaa aggatgttcc gcgaccgtta gagcaagcgc gtctcagcgc ttgctatcgc
    39001 ggcaatatcg ccagtggtgc tgtcgtgatg cggtcttcgc atggaccgca caatgaagat
    39061 acggtgcttc tgtatcgtac ttattgtttc tggtgcgctg ttttcccaag taaataataa
    39121 ccggagtctc tccggcgaca atttactggt ggttaacaac cttcagagca gcaagtaagc
    39181 ccgaatgccg ccctttaggg cggcatattt tagattatcc ggttttgttt aaagtcacgc
    39241 aaaaaaccac cccagcgacg ttcatagaat ggcacaatat gttcggtaat aaagtggcta
    39301 attccttttt cccctttttt cacctgacaa atatcgattg gttcatcgcc aggtaatgta
    39361 tcggtcgcta cacttcccgt cgcctgaata atttcttcga tatcaccatc ggcttcaatg
    39421 ccaataagta aattaggctg tgcctcttcg ttctctttaa ttgaacaaat aaaagcacgc
    39481 ttcaccggct taatggtttt aaataaggtg gtgagtgaat caatcatttg tgctggcggc
    39541 tctgcgactt ccgataatat cagcgattca ccgccttcca ggatttcctg gctgctcagc
    39601 ggatttccct cttcaccaat caacaaactg atttcacgcg gcataaattc tttaccggtc
    39661 ggcagtttgg cattaaggaa gagcgtttcg ccaagggtca tctcaaacaa tgtgcgaacg
    39721 ggcattacga caaatgcctg ttcgtcttca accgcctgtt gaagcgcttc taacgaggtg
    39781 aaaaaaggaa tgacgctggt gccgtcttct ttttcccagt gctgtaaatc aagcgcacta
    39841 tcttcaacca cagcctcgcc ctgcgccgcc gtaccaggca cccagacggt ggattccagt
    39901 agagtacgga aaaaggccgg gcggtgcgcc ggttcggttg ctgctttttc cagcaggtct
    39961 tcaagttcgt tttttgtttc ggacataaga accacaattc attcaacgtt cggggcgcaa
    40021 atgcctgatg cgctacgctt atcaggccta caagggattc gcaatttgtt gaatttgcag
    40081 aatttgtagg ccggataagg cgtttacgcc gcatccggca tattagttta cgccgttaac
    40141 agattagcta tcgtgcgcac accaagtccc gtagcgcccg cagaccactg ttcaaccggc
    40201 gctttacggt aagtcgccga gcagtcgata tgcagccagc cttgctgata gttctcaaca
    40261 aagtgcgaca ggaagcccgc cgccgtgctc gcgcctgccg gatacgccgc gcttccggta
    40321 ttgttcagtt cggcaaagtt agacggcagc tggctgcggt ggaattccgc cagcggcaga
    40381 cgccagaatg gttcgttctc ttgtgaggca ctcgccagca aacgaccggc aagcgcatcg
    40441 tcaaaactga acagcgcgtg ataatcatta cccagcgcag ttttcgccgc cccggtgagg
    40501 gtcgccgcat caatgatcat ttccggtttc tgcgcactgg catcaatcag accatcggca
    40561 agcaccagac gcccttcggc atcagtgttc atcacttcaa cttttttacc gttgcgatag
    40621 gtgatgatat cgcccagctt gaacgcattg ccgctaatca ggttatccgc acagcagagg
    40681 aacagcttca cgcgcttgtt cagtccgcgc gtaatggcaa atgccagcgc cccggtaacc
    40741 gttgccgcgc cgcccatgtc cgacttcatc gagtccataa acgcagtctg tttgatgctg
    40801 tagccgccgg agtcaaaagt gataccttta cctaccaggc acgcgtacac tggcgcttct
    40861 ttatcgccag ttgggttgta atccagcgcc agcaataccg gagaacgttc tgaaccgcgt
    40921 ccgacggtgt gcagccccat ataaccttgc tcacgcagat cttcgccttt ggtgatccga
    40981 taagtcacac gatcgcccgc gacgttgctg atcagatcaa cagcacgctg tgccagttgc
    41041 gatggtccca attcttctgc cggtgcgttg atggtgtcac gcacccagtc gatgatcatc
    41101 aggcggttat ccagttcctg gcgctgggca tcgtccagat ccggccactc cactttacgc
    41161 gtgcctttcg gggctttgta accttgccag aatgcccagc agcgatccgc atcccagcct
    41221 tcaccgctta actgaacatg cttgatgccc agaccgtcaa tcttgcgcgc cgcacgctgg
    41281 atcagcccca gatcgtctgc cccgttcaaa tgcagggtaa tgccgtcatt attaatgctg
    41341 taagttgctt tttctcccca gcgcgcgtcg gcaggttggg tagagagggt aatcttcatc
    41401 gcttctgtca ttttagttat ccttctttgt aaaagggccg ccagttggca gccccaggat
    41461 ttttccgcta aatgattcgc gttgcagaaa ggcggcaagt gagtgaagcc ccaggagcat
    41521 agataactat gtgactgggg tgaacgagcg cagccaacgc atctgcggcg tgaagcatga
    41581 cgcggaaatt attcggcctc gtccagccag actaacaaaa tcgcttcgag gattttctcg
    41641 ttggatgcct gcgggtcgtc gtcgaaatct tccagatcgc aaatccactg atgcatatcg
    41701 gtgaatcgaa ccgttttcgg atcaagatca ggatacgcat cgtacagtgc ttcgccaatt
    41761 tcgcggctat cggtccactt aagtcccata ctaacctctg ttaatgctca cgcgcatggt
    41821 tgatagtgta acgcgggatt tcgactacta aatcttcgtc ggtaacgcgt gcctggcagc
    41881 ttaaacggct ttcgggctcc agtccccagg ctttgtccag catgtcgtct tcctgctctg
    41941 agctttctgg cagcgagtca aaaccttcac gaacgatgca gtggcaggtg gtgcaagcac
    42001 aggatttttc acaggcgtgt tcaatctcga taccgttacg cagagctgcg tcgagaatgg
    42061 tttcaccgct attagcttcc agaacagcgc catcaggaca gagatcctga tgaggcaaaa
    42121 taacaatctt tggcatatta aacctcgtcc acggaatggc ctttcagcgc acgacgaacc
    42181 gactggtcca tgcggcgagc ggcgaaatcc tgggtttgtt tgtctacgtt tttaatcgct
    42241 tgttcgatgg cgtcaacgtc atcgccctgc gccacttcac tcaggtgagc ggcagcatcg
    42301 tcaatgacct gacgttctgc ggcgcttaac agcgcggcat cagcagccag cgcgccgtgc
    42361 agactttcca gcacacgcgc cgcttctact ttttgttctg ccagcattcg ggcttttacg
    42421 tcctgctcgg catagctcat tgagtctttg atcatcgaag cgatttcgct atcggtcaga
    42481 ccgtaagacg gtttgacctg aatagacgcc tcaacgccgg tggatttctc catcgccgtc
    42541 acgctcaaaa gaccgtcggc atcgacctgg aacgtcacgc gaatatgcgc accaccagcc
    42601 ggtagcgccg gaataccacg cagcgcaaaa cgcgccagtg agcggcagtc ctgcaccagt
    42661 tcgcgctcac cctgcattac atggatagac atcgccgtct gaccatcttt aaaggtggtg
    42721 aaatcctgag cgcgggccac cggaatagtg gtattacgcg gaatcacttt ctccaccagg
    42781 ccgcccatcg tttcgaggcc cagcgacagt gggatcacat caagcagcag catttcgctg
    42841 tctggcttgt tacccaccag aatatccgcc tgaatcgccg cgccaatagc gacgacttta
    42901 tccgggtcga tggaagtcag cggtggacga ccgaaaaatt cgcctacccg ttcacgcacc
    42961 agcggcacgc gagtagaacc gcccaccatc accacttcca gcacttcgtc agcttctaca
    43021 cccgcgtctt taagcgcgcg acgacaagcc agtaaggttc gttttaccag tggcgcgatc
    43081 agttcattga attgttcacg gctgatttcg ccctgccagc ccgcaacgtt aacggtcacg
    43141 gagtccgcat cgctcagcgc gattttggct gcaatggcgg catccagcag ttcacgctga
    43201 acgcggttat cgctacgatc aggaatgccc gcctgctcgc gaatgtaatc cgccagcaga
    43261 tggtcgaaat catcgccgcc gagcgcggaa tcaccgccgg ttgccagcac ttcaaacacg
    43321 ccgcgactta agcgcagaat ggaaatatca aacgtcccgc caccgaggtc ataaacagcg
    43381 atcacgcctt cctgaccgga atccagcccg taggcgatag ccgcagcggt cggttcgtta
    43441 agtaagcgca gaacatgcaa ccccgccaga cgcgccgcgt ctttggtgcc ctgacgctgg
    43501 gcatcgtcaa agtacgccgg aacggtgata actacaccat ccagctcgcc tgccagggct
    43561 tcagttgccc gcgccgccag tgctttgagg atgtccgcag aaacgcgcac cgggttcagc
    43621 agccccgccg ccgtttcaat catcggcagg ccgttttcgc tggcctggaa ttgataaggc
    43681 agatgcggat agcgttgctg gatatcagcc agcgagcgcc ccatcaggcg tttaacagaa
    43741 ctaatggtgt tggcggtgtc aagcgctgcg ttagtacgcg cgtcataacc caccgaatgc
    43801 ccttgctgtt gatagtgaac aacagatggc agcaggtgac ggccttcatg gtcggctaac
    43861 gtttcggcct gaccgctgcg cactgtcgcc accagcgagt tggttgtgcc caggtcaata
    43921 ccagccgcca gacgacgctg atgcggcgca gcactcaaac caggttcact aatttgtaat
    43981 aaggccatgt ttagcttcca gaaattaaaa atcgagcagt ttttcttcga gttgttcggc
    44041 actgctccgc agtttatcga gaaaacgcag cttacgcacg gtatccgccg ccgcgtccca
    44101 cgcctcgttg tctaactgtt caaccatcaa ctgatggcgg gtatcaaaca tctttttcac
    44161 acgtttgata aagctttcca gccgcgcttc atctttcgcc tgttcgatct cgtccagctc
    44221 ttcgcgcagc tccaactgtt ccatcaggaa cgcggtgtcg cgcacagtat gctgctcgct
    44281 ggcgagatca aaaccgtgta aagaaagcaa atattccgcg cgcattaacg gatgacgcag
    44341 cgtttgccag gcctggttaa tggttgcaga ttgctgtacg gcggcgagtt gttccgcctg
    44401 gcttccgctg gcgaatttat caggatgata ctgacgttgt agatcctgaa aacgcaggct
    44461 cagcgcctgg gtatcgagtt gataacgggc aggcaagcca aagaaggtga agtaatccat
    44521 aacattctca gggctgcgat ccgccgcgat aacggcgaat caggtaaaac aaaccccacg
    44581 cgcaggcgac cacggtgggg ttatcggtat gcgcatcaaa cgtggaagct ttcgccgcaa
    44641 ccacactcat ctttgacgtt cgggttggtg aatttaaacc cttcgttcag gccttctttt
    44701 acgaagtcca gctgcgtacc gtccagaaat tgcaggcttt tgccatcgac cacgactttc
    44761 acgcctttgt cttcaaacac gatgtcttcc ggcgtcggtt cgtcaacaaa ttccagtaca
    44821 taagccatac ctgaacatcc ggaggttctc acgcccagac gcaggccaaa ccctttgccg
    44881 cggttagcca gaaaggtatt tactcgcgct gctgcactgt cgctcagtgt aatcgacata
    44941 accaaacctc aactcttatt ttgcttcacg tttgctttta tagtccgcaa tggcggcttt
    45001 gatcgcgtct tctgccagaa tagaacagtg aattttcacc ggcggcagtt caagttcttc
    45061 agcaatatcg gtatttttga tcgcctgcgc ttcgtcgaga gacttccctt tcacccattc
    45121 ggtgaccagg gagctggaag cgatagcaga accgcagccg taagttttaa aacgcgcgtc
    45181 ttcaatgata ccttcatcgt tgactttaat ctgcaacttc atcacgtcgc cacaggccgg
    45241 tgcccccacc atgccgctgc cgacgttctc gtcgttgttg tcaaaggaac ccacgttacg
    45301 cggattctcg taatggtcga taactttttc gctgtaagcc attataaatt ctcctgattc
    45361 cgataccgat taatgatgag cccattcgat gctgttcaga tccacgccct gcttgtacat
    45421 ttcccacagc ggagaaaggt cacgcagacg accgatggat ttacgaacta actcgatggt
    45481 gtagtcgatc tcttcttcag tagtaaaacg acctaaagag aaacggatag agctatgtgc
    45541 cagctcgtcg ttcagcccca gcgcgcgcag cacgtaggac ggttcgaggc ttgctgacgt
    45601 acaggcggaa cctgaagaaa ctgcgaggtc tttcagcgcc ataatcagcg actcaccttc
    45661 aacgtagttg aagctgacgt tgagaatgtt cggcgcaccg tgttccaggt caccgttcag
    45721 gtaaacttct tcgatatctt tgatgccgtt ccacagacgg ttacgcaggc cgcgcagacg
    45781 ttccatctcg gtcgccatct cttcttttgc gatgcgatag gcctcgccca ttccgacgat
    45841 ctggtgaaca ggcagagtgc cggaacgcat accgcgctcg tgaccgccgc cgtgcatttg
    45901 cgcttcgatg cgtacgcgcg gtttacgacg tacatacagc gcaccgatac ctttcgggcc
    45961 atagattttg tgaccggaga aagacatcag gtcaactttc aactggctca ggtcgatagg
    46021 cagtttgccc acgctctggg ttgcatcaac gtgatagata atgccacgag cacggcacat
    46081 ttcgccgata gccgcgatat cctgcaccac gccgatttcg ttatttacgt gcatgatgga
    46141 aacaaggatg gtgtcatcac gcatcgctgc ttcaagttct ttcagatcga taatgccgtt
    46201 acgctgcggt gccaggtagg tgacttcaaa accttcgcgc tccagctgac ggcaggtatc
    46261 cagtaccgct ttgtgttcgg ttttgctggt gatgatgtgc ttgccttttt tctgataaaa
    46321 gttggctgca cctttgatcg ccaggttgtc agattcggtt gcaccagagg taaagacgat
    46381 ttcacgcgga tcagcgccga ccagatcggc aatctgatta cgggcgatat ctaccgcttc
    46441 ttcagcctgc cagccgaaac ggtgagaacg ggaggccggg ttaccaaagg ttccgtccat
    46501 cgtcataaac tgcatcattt tctcggcaac acgcgggtcc accggcgtgg ttgcggagta
    46561 gtcgagataa atcggtaatt tcattgctct ataaactccg tacatcactc aatgcaagga
    46621 accaggctac cggctggatg tacgaccgtg tttacggagc gcgatacgcc ccggcctgat
    46681 tctaatatct tgttatcttt aacctaactg tttcgggttt cagcaagttg acaaacttac
    46741 gaatctccgg gagcttacat aagtaagtga ccggagtgag tatgtgcagt caacgcagat
    46801 gcaacacgaa acaggacggt taaattaagc gcgtaactta acgtcgatcg cgtcttgtat
    46861 gcgggtgcgt ggcgcgtcgt gagtatgctg acgaccagac acatccagca cttcctggtt
    46921 attaaccagt tcgcctaaag taatgttgtt gagaaaaccg gtgagacggt cgctcaaatc
    46981 acgccacagc gcgtgggtca ggcatttatc gccgccctgg caaccgcctt taccctgaca
    47041 acgggtagca tctacagatt cgtcaacggc gctaatcact tcgccaacgg cgatgctgct
    47101 ggcatctttg cctaacagat aaccaccgcc tggtccacgt acgctggaaa ccagaccatt
    47161 tttacgcaga cgggaaaaca gttgttccag ataagaaagg gaaattccct gacgttcgga
    47221 aatatcagcc aacggtaccg ggcccgcttc agagttgagc gcaacgtcaa gcattgcggt
    47281 cacggcatag cgccctttag atgtcagtct catgtcttac ttcacctcaa actcgcccct
    47341 gcccggggtt ttttattgta aaagtggggg tattgcatag cagggtcaag tctgacattc
    47401 ccgagtaaat tggtcaacta tttacttgac tgatttagtc gggtatttaa ccttcagtgc
    47461 cattttttta tcgtggcgtt tgtacctgtt gtccttccag accatgggcg ggttgagggc
    47521 aattattcgc attagcgctg gcctcttccc ctcaccctaa ccctctcccc gaaggggcga
    47581 ggggaccgtc cgtgcattta tctggcttag ccctcaggcc ttattacctt tattctgctg
    47641 ctctatagaa gccagaatcc cgcgcaggat attcaattcc tggctttccg ggcgcgcacg
    47701 ggtaaacaga cggcgcaatt tattcatcac ctgccccgga tggttttcac ggataaaacc
    47761 ggttgccagc agcgtttgtt ccagatgacc ataaaaacgc tccagatcat cgaccagcgg
    47821 atacggcgtc tcttcatgct cgacctgctc gccattttcc tgagttgcca gccaggccat
    47881 acgcacttca taggcaataa cctgaaccgc cattgccaag ttcagcgaac tgtattccgg
    47941 gtttgccgca atcgcgacat gataatggca tttctgcaac tcttcattgg tcaagccgac
    48001 gcgctcacga ccaaacacca gcgccaccgg ggtatttgcc gcttcagcga cgcttttcag
    48061 accgcattcg cgcgggtcga gcatcggcca cggcagcgtg cgggagcgtg cgctggtgcc
    48121 caccaccagg ctacaaccgg ctaacgcttc gtcgagcgta tcgacgatgt gagcattacc
    48181 aatcacatcg ctggcccctg ctgccagggc aatcgcctgg gagtcgggtt tcaccagtgg
    48241 attaaccagc cacagattgg ttaatcccat tgttttcatg gcacgggcaa cagaacccat
    48301 attgccggtg tgtgacgtct ccaccagcac aattcgaata ttttgcagca ttgtctttct
    48361 tcatctaaag attattcacg catcttatca taaaacgaag acagatgccg atctcgctgc
    48421 tatactctgc gccgttttcc cgttctttaa catccagtga gagagaccga tgcatccgat
    48481 gctgaacatc gccgtgcgcg cagcgcgcaa ggcgggtaat ttaattgcca aaaactatga
    48541 aaccccggac gctgtagaag cgagccagaa aggcagtaac gatttcgtga ccaacgtaga
    48601 taaagctgcc gaagcggtga ttatcgacac gattcgtaaa tcttactcac agcacaccat
    48661 catcaccgaa gaaagcggtg aacttgaagg tactgatcag gatgttcaat gggttatcga
    48721 tccactggat ggcactacca actttatcaa acgtctgccg cacttcgcgg tatctatcgc
    48781 cgttcgtatc aaaggccgca ccgaagttgc tgtggtatac gatcctatgc gtaacgaact
    48841 gttcaccgcc actcgcggtc agggcgcaca gctgaacggc taccgtctgc gcggtagcac
    48901 cgctcgcgat ctcgacggta ccattctggc gaccggcttc ccgttcaaag caaaacagta
    48961 cgccactacc tacatcaaca tcgtcggcaa actgttcaac gaatgtgcag acttccgtcg
    49021 taccggttct gcggcgctgg atctggctta cgtcgctgcg ggtcgtgttg acggtttctt
    49081 tgaaatcggt ctgcgtccgt gggatttcgc ggcaggcgag ctgctggttc gtgaagcagg
    49141 cggcatcgtc agcgacttca ccggtggtca taactacatg ctgaccggta acatcgttgc
    49201 tggtaacccg cgcgttgtga aagccatgct ggcgaacatg cgtgacgagt taagcgacgc
    49261 tctgaagcgt taatgactca ggcgggtgat gtcactcacc cgccctcgcc tttcaggcgc
    49321 tattctgaaa tacttcctca ccgctttact ttctttcgtc actctcccac catctttccc
    49381 cgattaatgg atgataaaga agtcacaagg aaaggttatg gcgctgccag tgaacaaacg
    49441 cgttctcaaa attctgttta ttctctttgt tgttgccttc tgcgtttatt tagtgccgcg
    49501 cgttgccatc aacttcttct attatcctga cgataaaatt tacggtcccg acccctggtc
    49561 ggcggaatcc gtcgaattta cggctaagga cggtactcgt ctgcaaggct ggtttattcc
    49621 ttcttcgacg ggccctgctg acaatgccat cgcaaccatc attcatgctc acggcaatgc
    49681 cggaaatatg tccgcccact ggccgctggt cagttggtta cccgagcgta atttcaacgt
    49741 ttttatgttt gattatcgcg ggcttggtaa atcaaaaggc acgccgtccc aggccggatt
    49801 gctggacgat acgcaaagtg ccatcaatgt ggtgcgccat cgcagtgatg taaacccaca
    49861 acggctggtg ctgttcgggc agagtattgg cggggcgaat attcttgctg ttattggtca
    49921 ggatgatcgt gaaggcatac gtgcggtgat cctcgactcc acatttgcct cttatgcaac
    49981 catcgccaat cagatgatcc ccggcagtgg ctacttactt gatgagagtt acagcggcga
    50041 aaattatatc gccagcgtca gcccgatccc gcttttactc attcacggta aagctgatca
    50101 cgttatccca tggcagcaca gcgaaaagtt gtatagcctg gcaaaagaga caaaacggtt
    50161 gatcctaatc ccggatggcg aacacattga tgctttttcc gatcgtcacg gcgatgttta
    50221 tcgcgaacag atggtggact ttatccttag cgcgttgaat ccgcagaact aacccatgat
    50281 cgctagcacg ataatcattc acaaaaccac cttaagacat gctaatccac tggtcagaac
    50341 agtttaagat gagaaaaatt ctgtgacgct tgccaacatt tctgatgatt agcattccct
    50401 tcgccatttc cctgagcaaa ctttagctat tcttatcaat tatgcttatg ggagatctac
    50461 agatgatgcc tacgcttgct ccaccatctg tcctttcggc tccccagcgc cgctgccaga
    50521 tcttgctgac gctctttcag ccggggttga ccgccaccat ggcaaccttc agcgagctta
    50581 atggtgtgga tgatgatatt gccagtcttg atatcagcga aacaggacgg gagatcctgc
    50641 gctatcatca actcacactg acgactggtt atgacggtag ctaccgggtt gaaggtacag
    50701 tgcttaacca acgtttgtgt ttatttcact ggctacgacg tggtttccgt ctgtgtccgt
    50761 catttattac cagccaattc acccccgccc tgaagagtga actgaagcgg cgcggaattg
    50821 cgcgtaactt ttacgacgat accaatctac aagcgttagt gaatctctgc tcccgacggc
    50881 tgcaaaaacg ctttgaaacg cgcgatattc atttcctgtg tctgtatctg caatattgtt
    50941 tgctgcaaca ccacgctgga attacgcctc agtttaatcc gctccaacgt cgctgggcgg
    51001 aatcctgcct tgaatttcag gtagcgcagg aaattggacg ccactggcag cgtcgggcgc
    51061 tccagcctgt accgcctgat gagccactgt ttatggcatt acttttttcc atgttgcggg
    51121 ttcccgatcc attgcgtgat gcgcatcagc gagacagaca attgcgtcag tctatcaaac
    51181 gtctggtaaa ccattttcgt gagctgggaa atgttcgttt ttatgatgaa caggggttat
    51241 gcgatcagct ttatacccac ctcgcccagg cgttaaatcg cagtttgttt gccatcggta
    51301 ttgataatac cctgccggaa gagttcgcca gactgtaccc acgcctggtg cgcaccaccc
    51361 gcgcggcgct ggccggattt gaaagtgaat acggcgtcca tctttctgat gaggaaagcg
    51421 gtctggtcgc ggtgattttc ggtgcctggc taatgcagga aaacgatctg catgaaaaac
    51481 agattattct actgaccggg aatgatagcg agcgagaagc gcagattgag cagcagttac
    51541 gcgaactaac gttactgccg ctcaacatta agcatatgtc ggtaaaggca tttttgcaga
    51601 caggcgctcc gcgtggcgcg gcactgatta ttgcgcctta taccatgcca ttaccgctct
    51661 tttcaccacc gctgatctat acggacctga cgctgacaac acatcaacag gagcagatcc
    51721 gcaaaatgct cgaatcagca tgagggaaca actttcgggc gcaaaaacat tgccggaagc
    51781 gccaccagcg ccattaccca gaacacgccg tggcccagat attgatacag gaaaccggcg
    51841 aaaacggtca tgatagcgat actgccgccc atcgcgacgg cagagtaaac cgcctgtaaa
    51901 cggatgactt cgctaccctg gcgaacagca atatagcgca tggcggccag gtggcagacc
    51961 gtgaaggtgc cgcaatgcaa aatttgcacc actatcaacc acggcaacgc cgtagttgct
    52021 cccataatgc cccagcgcac tacgccgcaa atcgccgaga tcaacagcat atcgcgagca
    52081 ctacaacggc ggaaaagttt attactcagc gcaaagataa tgacttccgc caccacgccc
    52141 agcgaccaca aataccccac cgccgaggcc gagtagccag ctgcctgcca gtaaatggcg
    52201 ctaaaaccgt aataggccgc atgtgccccc tgcaataaac aaacgcaggc cagaaagcgc
    52261 cagttctggc gaaccagcgc caaccacgca gaccaaccgg tgctctcctg ctggcgactt
    52321 gccccttgtg gctgaatcgt cggacggatg agaaagccga gcagcatgga tgccacgccc
    52381 aacgtcaaca gcgcgaggat cacccgataa tcaaacatag tgaccagttt gcccgtcagc
    52441 gccgagccaa tgacaaacgc caccgagccc cacagtcgca ctttgccgta atcaagcggg
    52501 aactgctttt gccacgtatt cgccagtgca tcggtcaacg gtaccagcgg tgagaaaaag
    52561 aggttaaagc caatcatcac cagcatcagc cacgctacgt gcgcccccgc ccagaaggcg
    52621 acagcaaaga gaagtgtcag cagtgccagc acgcgcaagg cggaaatcag gcgggaagga
    52681 tcgctgacgc ggggcgcgat gagcaaactc ccgagaaaac gggcaaccag acctgccccc
    52741 aataacaggc cgatggtttc tggcgttaaa ccaatccctt taagccagac gctccagaaa
    52801 ggtagaaaaa tgccgtaact aaaaaagtat gtgaaatagc cgagcgccaa ccagcgcgtg
    52861 gattgcaaaa ccatgagtcc ctcccgtcag gaggcgttag tctggggtta atcgaatgtt
    52921 ggtgcaagtt gaaaaataag aatattcgag cctgcttccc gatgtctgta acgccagttc
    52981 acaagcacta aaaaactgcg atccgcgacg catgttgcaa aaataatgcg taattatgcc
    53041 gttacgcttg ccaaacgttc ctgcacaatg gcgatgaaat cgcgtaatgc cggcttcatc
    53101 tcccctttct tccacgccat cagtaaagca atggaaggca catttccggc aatgggacga
    53161 aaaacaacct gcccggtatt aaaattattc atataaccgg gtatcaaagt gacgcccagc
    53221 cccatgccca ccagattcat ggtcaccaga atattcgttg ccacctggac gatatttggc
    53281 tggctgtttt cttgcgcaaa ccacgcttta acgatcggcg caagcgaacc ggaatacgcc
    53341 ggatcggtac tgacgaaatt cacgccatcc agttgcgcgg cggtgatctc tttttcatgt
    53401 gctaaagggt gatcaaccgg taacaccacc actaatggtt cgtcaaaaag ctccagataa
    53461 tcaatctccg ggctataaac aggatggcgc attaagccga cgtcgagttc accacggcga
    53521 attttttcct cctgttgcgt ggtgattaaa ctcaccagct caatcaaggt gtctggctgt
    53581 ctgagacgaa acattggtaa tacttttggc aataaattca cttccgccga tggcacaaag
    53641 ccaatggtta attgtctgtc ttcctgaaca attttccgcg cccgtaattt ggcattttcc
    53701 gcttgttcga ggattgccag cgcgtcctgg agaaaacatt ctcccgccgc cgtcaacgcg
    53761 actttgcgct tatccctcac cagtaacgga acaccgacac agttttcaag atcgcggatc
    53821 tggctgctta acgaaggctg tgaggtatgc agtttttccg ccgcacgggt aaagttcagt
    53881 gcctgcgcca ctgcgacgaa atagcgtaaa tgccgtagtt ccatcacctt ccccttgtta
    53941 tcgaaaaaac gtctcagctg gtagaaatta actatttcac atattagcaa ctaaccagca
    54001 acatccttat gacacaaaaa tagaaggtca atacatctta tctttcagga ttaaaaaata
    54061 tgaccacacc ctcagattta aacatttacc aactgattga tacccaaaat ggtcgggtca
    54121 ctccgcgtat ttataccgac ccagatattt accaactgga gcttgaacgt attttcggcc
    54181 gttgctggtt atttctcgcc cacgaaagcc agatcccaaa acctggtgat ttctttaaca
    54241 cctacatggg ggaagatgcg gttgtcgtag tgcgtcagaa agacggcagc attaaggcgt
    54301 ttctcaacca atgccgccac cgggccatgc gtgtgagtta tgcagattgc ggcaacactc
    54361 gcgcctttac ctgtccgtat cacggctggt cttatggcat taacggcgag ttgatcgatg
    54421 taccgctgga acctcgcgcc tacccacaag ggttgtgtaa atcccactgg ggactaaacg
    54481 aagttccttg tgtggagagt tataaagggc tgatttttgg caactgggat accagcgcac
    54541 cgggcctgcg tgattacctg ggtgacattg cctggtatct ggatggcatg ctggatcgtc
    54601 gcgaaggcgg caccgaaatt gtcggcggcg tacaaaagtg ggtgatcaac tgtaactgga
    54661 aattcccggc agagcagttc gccagtgacc agtatcatgc tctgttcagc catgcttctg
    54721 ccgttcaggt attaggggcg aaagatgatg gcagcgataa gcgcctcggt gatggacaaa
    54781 ccgcccgccc ggtgtgggaa accgccaaag atgcgctgca atttggtcag gacggtcacg
    54841 gtagcggttt cttctttact gaaaaaccgg atgctaatgt ctgggtcgat ggcgcagttt
    54901 caagctatta ccgcgaaacc tatgccgaag cagaacaacg tttaggtgaa gttcgcgccc
    54961 tgcgcctggc gggtcataac aatattttcc ccacgctttc atggctcaac ggcactgcca
    55021 cgctccgcgt ctggcatccg cgcggccctg atcaagttga agtgtgggcg ttctgtatta
    55081 ctgacaaagc cgcctccgat gaagttaaag ccgcttttga aaacagcgcc actcgtgctt
    55141 ttggtcctgc tggttttctc gagcaggatg actcggagaa ctggtgtgaa atccagaaat
    55201 tgcttaaagg ccaccgcgcc cgcaacagca aactgtgtct ggaaatgggg cttggtcagg
    55261 aaaagcgccg cgacgacggc attcctggca ttactaacta tatcttttca gaaactgccg
    55321 ctcgtggaat gtaccaacgc tgggccgatc ttctgagtag cgaaagctgg caggaagtgc
    55381 tcgataaaac cgccgcttac cagcaggagg tgatgaaatg agtgcgcaag tttcactaga
    55441 gttacatcac cgcattagcc agtttctctt tcacgaagcc agcttactgg acgactggaa
    55501 atttcgtgac tggctggagc agctcgacaa agagattcgt tacaccatgc gcaccacagt
    55561 taacgcgcaa acacgcgacc gccgcaaagg cgtccagcca ccgacaacct ggatttttaa
    55621 tgacaccaaa gaccagctgg agcggcgaat cgcccgtctg gaaacgggca tggcctgggc
    55681 agaagagccg ccgtcacgca cccgtcactt aatcagcaac tgccagataa gcgaaaccga
    55741 catcccaaac gtatttgctg tgcgggtaaa ttatctgctt tatcgggcac aaaaagagcg
    55801 cgatgaaaca ttctatgttg gaacgcgttt cgacaaagtt cgccgtctgg aagatgacaa
    55861 ctggcgcttg ctggaacggg atatcgtcct ggatcaagcg gtaatcactt cccataacct
    55921 gagtgtactg ttctgatgaa tcgaatttat gcgtgtcccg ttgcggatgt gccggagggt
    55981 gaggctctcc ggatcgatac ctcgcccgtc atcgccctgt tcaacgttgg cggcgagttt
    56041 tatgccatta acgatcgttg cagccatggt aatgcgtcga tgtcagaagg gtatctggaa
    56101 gatgacgcca cggtggagtg cccgctacac gccgccagtt tttgcctgaa aacggggaaa
    56161 gcgttatgcc tgcccgccac cgatccgctc accacttatc cagtacacgt tgaaggtggt
    56221 gacattttca tcgacttacc ggaggcgcag ccatgagcga tctgcataac gagtccattt
    56281 ttattaccgg cggcggatcg ggattagggc tggcgctggt cgagcgattt atcgaagaag
    56341 gcgcgcaagt tgccacgctg gaactgtcgg cggcaaaagt cgccagtctg cgtcagcgat
    56401 ttggcgaaca tattctggcg gtggaaggta acgtgacctg ttatgccgat tatcaacgcg
    56461 cggtcgatca gatcctgact cgttccggca agctggattg ttttatcggc aatgcaggca
    56521 tctgggatca caatgcctca ctggttaata ctcccgcaga gacgctcgaa accggcttcc
    56581 acgagctgtt taacgtcaac gtactcggtt acctgctggg cgcaaaagcc tgcgctccgg
    56641 cgttaatcgc cagtgaaggc agcatgattt tcacactgtc aaatgccgcc aggtatcctg
    56701 gcggcggtgg cccgctgtac accgccagta aacatgccgc aaccggactt attcgccaac
    56761 tggcttatga actggcaccg aaagtgcggg tgaatggcgt cggcccgtgt ggtatggcca
    56821 gcgacctgcg cggcccacag gcgctcgggc aaagtgaaac ctcgataatg cagtctctga
    56881 cgccggagaa aattgccgcc attttaccgc tgcaattttt cccgcaaccg gcggatttta
    56941 cggggccgta tgtgatgttg gcatcgcggc gcaataatcg cgcattaagc ggtgtgatga
    57001 tcaacgctga tgcgggttta gcgattcgcg gcattcgcca cgtagcggct gggctggatc
    57061 tttaaggaag cacgatgaaa gaaaaaacga tcattattgt cggtggcggg caagcggcgg
    57121 caatggctgc ggcctcgcta cgccagcaag ggttcaccgg tgagctgcat ctgttttccg
    57181 atgagcgaca tcttccttat gaacgacctc cactctcgaa atccatgttg ctggaagatt
    57241 ccccgcagtt acagcaggtg ttacccgcta actggtggca ggaaaacaat gttcatctgc
    57301 attccggtgt aaccatcaaa acgctgggtc gcgacacacg agagttagtg ttaaccaacg
    57361 gcgaaagctg gcactgggat cagcttttta tagcaatcgg cgcggcagct cgaccgctgc
    57421 cgttgcttga tgcactggga gaacgctgct ttaccctacg ccatgccggt gatgccgcca
    57481 gactgcgaga agttctgcag cccgaacggt cagtcgtgat tgtcggtgcc ggaactattg
    57541 gtctggaact ggctgccagc gccacgcagc gcagatgtaa ggtgacagtg attgaactgg
    57601 cggcaaccgt catgggccgt aatgcaccac cgcccgtgca acgctatctt ttacagcgcc
    57661 accagcaggc tggtgtgcgc attctgctca ataatgccat tgaacatgtg gtcgatggtg
    57721 aaaaagtaga actgacgctg caaagtgggg aaacgcttca ggctgatgtg gtgatttacg
    57781 gtattggtat cagcgccaac gagcaactgg ctcgcgaggc caaccttgat actgccaatg
    57841 gcattgtcat tgatgaggct tgccgcacct gcgatcccgc gatctttgcc ggtggcgatg
    57901 tggcaatcac tcgtcttgat aatggtgcac tacaccgctg cgaaagctgg gaaaacgcca
    57961 ataaccaggc gcaaattgcc gctgccgcaa tgttagggct accgctaccg ctactgccgc
    58021 cgccgtggtt ctggagcgat cagtacagtg ataacttaca gtttattggc gatatgcgtg
    58081 gcgatgactg gctttgtcgt ggcaacccgg aaactcagaa ggcgatttgg tttaatctgc
    58141 aaaacggcgt gcttatcggt gcggtcacgc tgaatcaggg gcgtgagatt cgcccaattc
    58201 gcaaatggat ccagagcggc aaaacgtttg atgcgaaact gctgatagat gagaacatcg
    58261 cgcttaaatc actgtaacca ggataattag cgaatatctc aacgcctggg gcgtggcgag
    58321 gtgcaagagt gtgtattacg tttaaatcac attatcttgc aaagggattg gttatgaaca
    58381 cactacgtta ttttgatttt ggagctgccc gccccgtttt gttattaatt gcccgtatcg
    58441 ccgtggtctt aattttcatt atttttggtt ttcccaaaat gatgggcttt gacggtacgg
    58501 tccaatatat ggcctcgttg ggcgcgccaa tgccgatgct ggcagcgatt attgcggtag
    58561 ttatggaagt gcccgccgcg atattaatcg tgcttggctt tttcacccgt ccgctggcgg
    58621 tgctgtttat tttctacacg ctgggtacgg cggtgattgg tcaccattac tgggatatga
    58681 ccggcgatgc ggttgggcca aatatgatta atttctggaa gaatgtcagt atcgctggcg
    58741 cgttcttgct attggcaatt accgggccgg gggcaatttc tctcgatcgg cgttaggaaa
    58801 gatgccggat gcggcgtgaa cgccttatcc ggcatttaat aaattacagc cactctaccc
    58861 gcaacgacat ctctgaagtt gttgattccc ctggtgctag cgcaatgaga tcaccccctt
    58921 ccgggcgatg atgatcatcc ggcgcatggc tcatcggttc cagacagaag aaatcaaacg
    58981 catatccttt atcaaacgca gggtcggaaa caaagatgaa ataacacggt gcaggtggcg
    59041 tcgtttccat gatgatggca taaccttcct gcggctgctc gatgcgggcc tgaccattcc
    59101 atccggcaaa accattgttc acccactggc gcggcaacgg cgcgagctgg ttaaaatcca
    59161 gttcctgcgg tagctgctcg caaaactcac ccgccagcca ctgctcccgt tccagccagt
    59221 aaccgctcgc ctgcgcctga atccgcgttt gtggtgacaa cggaaaataa ggatgccagc
    59281 cggtaccaaa tggcagcgtc tctgcccctt gattggtgac agagagcgtc accgtcagcg
    59341 tatccgccgt taaatgaaac gcctgactta cccgatagtg atagacaccg ctgcgatgtt
    59401 catacaccag acacaaacta tcatcgctgt gcgagacaca ttgccactcg cccagccagc
    59461 catcgccgtg tagatagtgc gcatcccact caacattcgg ttgcagttga tactcacgcc
    59521 cctgccagac aaaacggttg ccgctcaccc ggtttgcaaa tggcaccagc ggaaaacatg
    59581 aggcatcggt tgccacaccg ctttttttac caggacgtaa gagcggcgtc gtatcgcgcc
    59641 agaagccttc gattacgccg ccctggtcag aaacgtccag ctttagcgac ccgtgggata
    59701 aggtatagat ggtcatccgc taactcctta atccgggaag ttaatcacaa ctttcccgca
    59761 tttgccgctc gccatcagcg cataggcatc acctgcctgt tccagcgaga agcgatgggt
    59821 aatggcgtta cgcggccaca gcttccagtc agtcaaatcg tgggcgcatt tttccatatg
    59881 gaacagactg gtcacccagg agccgataat ccgccgttga tggtgcatca gatcggcgct
    59941 gacctcgaat tccacttttc cggtttcacc aatgtaaacc acccgtcccc agtcagcggt
    60001 ggattgcagt gccagcaagc gacctgcggc attaccagaa caatcgagcg caacatccgc
    60061 gccaccgtgg gtgagttcgg cgataatctg cggcagacct tcggtggttg ctaaatagcc
    60121 gtgatccatc acccctaact gttttgccat cgccagacgt tccggcagca tatcaacgcc
    60181 gatgatccgt tttgcaccgc gacctttcgc cagcatcatc gccatcatgc cgaccggccc
    60241 cagaccgacc accagcacgt tatcgctgcc ggaaacttcg ccgcgcaaaa tcccttcata
    60301 ggccgtgcca acgccgcaac tgataaacgc accatcttcg tagctcagcg catccggcag
    60361 gaggatcaga tctttttctt ccgccagcag atattcggca tgaccgccgt cacgctgcca
    60421 gccgtaagcc gcttttcctt tgccagtaca agagatagga aagccgcggc ggcagttcgg
    60481 gcaaaaacca cagccagaaa tgtgatacac cagcacgcgg tcgccctctt taaaatggcg
    60541 gcagccttgc cccatcgcca caatctgccc gcacggttca tgaccgttga taaatccctg
    60601 gtataacggt ttatcgggtg ccgccgccgt ggcacggtgt tgatgataga tatagtggac
    60661 atcgcttccg caaatcccgg aggatttcat tttgatcagt acctggttaa tccccggcgt
    60721 cggcaccgca acttcccgca gatcgacggt tgaatttcct ggtaaataag ctgccagcat
    60781 cgttttcata aatccctcat taacaatacg attaattttc atccctgccg cacccgcgcc
    60841 aggggcgtta aggtttagcg tttcgcttta ctgcttcgct gggtcagcaa gatattcgcc
    60901 agcaccgcca ccacgatgat gacgccgcgt accacctgct ggaaaaagga gttaataccg
    60961 agcagcacca gaccgttacc gattagcgta atcaccagca caccaagcaa tgtaccgaac
    61021 agggaaccgc gaccgccgga aagtgccgta ccgccgacca cgaccgcggc gatgacgtca
    61081 aactccagac cgtttgcggc acctgcgtta ccggaaccga ggcgtgccgc cagcaaaatg
    61141 ccggtcaccg ccgctaataa tcccgaaagg gtaaagataa gaatgcgcac ccgacgaacg
    61201 ttaataccgc acaactgcgc cgccgtggca ttaccgccaa cggcaaaaac cgagcgcccg
    61261 aaggcggttt ttcggctaat gaacacaaac agcgcaaaca acaccatcat gatcagcgcg
    61321 gatactggca caccgagaaa ttgtccgccc agccagtcca gcacctcgtt ttcgtcaatc
    61381 ggcactggca gtgcgttggt cataaacagc cccattccgc gcagggcgct ccacagcccc
    61441 agtgtggcaa caaaacttgg tacgttaaac acgccgcgca gcaccccggc gagcgttccc
    61501 atcagcgcgc ctaacagcaa caccagcaga caagcaatcg ccaacggaac ttcaaattgc
    61561 agcaaaaatg ccaggcacac cgagacaaaa gccaccatcg gcccaacgct gacatcaatt
    61621 tcaccggaga taataatcag cgtcatcgcc caggcggcaa tcccaatggt ggcggcatcg
    61681 cgcagcacgt tcatctggtt attcaatgag ataaagccag gcgcgttcag ggagaagacc
    61741 agataaagaa tggcaatcac caccagcaaa ccgatctcat taatatggcg actgacaaat
    61801 tgtttgagcg agacgctctt gccctgcggc aatggtaatg acgaagcaga catcttcagg
    61861 ttcctcggta aaattcatca gtgcacagac agaatggcgg acatcagctc atccacattg
    61921 accggcgagt gaaactcctg cgagaacgtg ccgtgctgta ataacaggat gcggtcacac
    61981 accagcggca actcctccac ttcactggag ataaacacca cgctttttcc ttcggcagcc
    62041 agctcacgga caatacggta aatctgctgt ttggcttcga tatcgacgcc gcgcgttggc
    62101 tcgtcgagca acaaaatctg gctggcggca tagacccaac gaccgatcac cactttttgc
    62161 tgattgccac cagaaagcgt gccgatgggc gtttcgctac tagcggcctt gaccgtcatc
    62221 cgctgcatca cctcttcggt caggcggcgg atggtggacc attgcagcac tccgttggcg
    62281 ctgatttttt gccgattggt cagcactgta ttttcgtcaa cgcccaacca gggaatgatc
    62341 cccgcttctt tgcggttttc tggcgtatag ccaatgccgc gtttcagcat gtcgccgtaa
    62401 tcggggcgcg tgattttctc gccgttgata acaatttcgc cctgttcata cgtctccagc
    62461 ccaacaatcg ccttcagcaa ttcactgcgc cctgccccca gcaggccagc aatgccaagc
    62521 acttcgccgc gacgtaacgt aaagctgata tcctccagct tgggcttatg gcgtaacgca
    62581 cggacttcca gtacggcctg atccacaatt tcctgagggg caaccggcgc aatatcaacg
    62641 tgatcacgcc ctagcattag cgacacaata tgatgcgtgg aggtgttttc gagcatcaca
    62701 tcgcccgcca cctgaccatc gcgcataacg gtggcacagg aggcaatgcg gcggatttct
    62761 tccatccggt ggctgacata aatcaccgcc acgcccagtg ccgacatctt tttcaccgca
    62821 ctgatcacca gttcaacttc tgcactggca agcgagctgg tgggttcatc aagaatgacc
    62881 acgcgcggct cgcccttcat cacccgtgca atttccacca gttgcttttg cgccgggctt
    62941 agcgttgaaa caagttgttc aggactaacg tcaacgccca gcgcctgtaa gcaacgttgg
    63001 gcatcctgcg ccatttgcag gtaatcaatc atgccgttgc ggcgaggcca ttgaccaagg
    63061 cagaggtttt ctgccaccgt cagcccttcc accagactta attcctgata aaccgcacga
    63121 acccccagtt cagcggcacg gcgagtcaac gtagcttcgt caccttccag tcgcgtctcg
    63181 ccaatccaga tatcaccgct atccgggcgt tcgctgccgg taagcattcg aatgagagtc
    63241 gatttgcccg cgccgttttt acctaacagc gcacgaactt cgcctttatt gagcgtgaag
    63301 ttaacgttat ccagcgcaac gacgccgggg taacgcttat ttcctgccac cacttttgct
    63361 accgggactg cctctgttgc cgtgaacata gggacctctg cgaatcagcg attagggcag
    63421 accatcaacg tgcgttgcca gccactgttt accatcttcc gttttggtat agagatcgat
    63481 tggcacctga atcacttttt caccgtcggc ttgtttattg ataaccttca atgtttgcgc
    63541 gaaaacagca ttgcccattt tcttaccgga aatatccact accgctttca gcacctgatt
    63601 gttttccagc tcctgagcaa tttcggttgt catatccgaa ccgaaaacag caatttttcc
    63661 ggcctgattt tgattacgta ccgcttttac cgcgccgagt gtcgcaccgc ccgactcccc
    63721 cataatggcg ttgagatccg gcgtggagat aatcagtttt tcaccaacgg aaatcgcttt
    63781 atttaaaaca gtcccttcct gattagcgac aatttgcgcg ccgggaacgc gggattttaa
    63841 tacttcttca aatcctttac gtcgctgcac acaaacttca aaggcttcgc aattgatgac
    63901 ggcaattttc ggctggtcaa ttttattggc aataaaataa tcggcggcag cgttacccag
    63961 ttttttacca aattccagtg gatcgccgac cagatacgcc gagacatatt tatcgacacc
    64021 cttttgatta atacaggtgt tgtagcaaat caccggaata cccgcttcac tggcgcgacg
    64081 aacggtacgg ctactgccat tttcagacac tgccgataaa ataatggcat cgacattacg
    64141 cgccacgagg gtatcaacaa aggtactttc tttcgaaata tcaccctggg cgttagtttc
    64201 aattaactgt acctgtactg aagaatcttt tgccgcatcc tgaacgccct gacgcactcc
    64261 agcgtaatat cccagggtat caaggtatat tgcaccaatg gtcatttctt tatccgcagc
    64321 cctggcaaat agtgcgctac ctaatagcgt agccattaat aatagattac gggttgttct
    64381 cattttttta ggcatagtgc ctcctgtagg gtttttatta acaacggctt attctaatta
    64441 ttttgtgatg agcggcagcg cgtgccgctc aaatattaca gttaataggg tttagtgaac
    64501 gtagttaaaa ataaaaggca tcacggtggt gaataatgcc gctttcggcc aggccggatt
    64561 aatcgccgtc aattcagcgc gagcagattc atattgcgca acctccccga gtcccgcgga
    64621 cgccagcatc gccaccatca ggcatttttc tttatgctgc tgttgtaaat cgccatacag
    64681 cgacaacagg tcaggttgtg aaacagcaaa gaaatccgcc tcaatactgg ttttcgccat
    64741 ctcttgcgcc cactgtttca tttcactaaa cagttgctgt gcgatttgtt gttcgcccag
    64801 cagtcgcagc gccattcctt gccagaagag ataatcaacc ggctgatcgt tgtaataact
    64861 gtggatgtta atggtgcgat cgccggtcgc cgccagacgt aaacaacgcg tcgcttcagt
    64921 ttcatcgccc tgcgcgttgg cgcatatcgc ctgccagaac cagatgtcgt tatcagtttg
    64981 ccccggtaaa cggccttcgc ttaaattctc cggataatgc agcgcggcat gaagcagttc
    65041 gcaggcctgc tgcggctgtc tggcatcaag atgctgccag gcgcgtaata actggttgag
    65101 gataaactga ctggtgacct tcccttcccc gccttcccac gggtggaatt tgcgcgtggc
    65161 gagaatgtcc gccgctttgt ctgcctgacc cgtgagatgc cacaaattga gcagttctgc
    65221 ggtcatgtcg tcgcgtttca gcgcaatttc cagattattt tccagacgcg ccagtcgttt
    65281 ctccggtgtg gctccactta acttatccag caaatcccgt tcgaaaagca gacgtgcatc
    65341 ctgcggcgca agctgataag cattatcaag ataacgcgcg gccagttcat aatcgtgttg
    65401 cttattccac gcatggatcg ccaacccgcg ccagccatcg gcaaactccg gcgacatctc
    65461 tacgcaacgt tgccataaag taatggcttt gttgtagctg cgcttgttgt agtagaagca
    65521 ggccagtaaa tgacgggcaa accagcactc ttcaatactc tccagcgccg ccacttcttc
    65581 cagcgtattc gggaaacgga caaactgcgg gaagacatca atggcttttg cgaccagttc
    65641 gccgcgttcg gctttcggca gcaagctggc ttgtagataa agcgataacg tgcgctgaca
    65701 gtccagtgcg ttcagcatct ctgtcgccag agtgggcatt ccccagttaa tcagttgccc
    65761 ggcggtcatc agggcgttaa cgtcgcgtcc ctgacacaga ccgcgccact gcgcgagggc
    65821 tgattcgcta cgaccatcga accagttcag ccaccacaga atggcgttca gcggataatc
    65881 acgcagcagt ttcgcgcgct gcaaacgcgc gttgtcctga cgaccactta acaccagcag
    65941 cagattatgc aggcaaagca cttcctgatt ggttgggcag gcgcgaagac tttgttggca
    66001 aaaatccaga ccagcgtcga agttaccatt acgcgccgcc agtcgtgcca ggccataata
    66061 gccaccggct ttgctgttgc cgctccagac cgcacgccag aaatcctctt cggcttgttg
    66121 atattgtccc tgacgttcgt aagcactggc gcgaatcaaa cttgcttgtc cacactgcgg
    66181 gtttttgttc agcgcatgtg cgcgtttcag cgcctgactg gcatacgcca ccgcttgcgg
    66241 gaaatctgcg cggttatatt ccagcatcgc cagcgccagg ttacagcgat aatccagcgg
    66301 gtccagcgcc acgccgcgca ggtagtaatc gaacggtgaa cggctggcgt gatgatattg
    66361 ctccagatgc tgaccgataa accaggcttc atctgtactg gtaatgtctt gtgctgccag
    66421 tggcgctttg gcgacgtccg gtaacggcaa cgcttgcggc tgatgttcct gataactcag
    66481 tacaatattg ccatcggcat cggaaagctc aatggtcagc ctatcaggat tgataccgtg
    66541 caacacgccc tggatggcgg tcgcaggcat cagtgccacg gcatcatcaa gtaacgcgtt
    66601 gcatttgccg atttcgcgga tcgccaggcg gtatccgttc aacggagaga tggcatacag
    66661 cccccactca atcccctgct cactacgctg gagttttatc accgcatcgc gggaggcatt
    66721 ttgcaccatg cccagagaat gataaggcag gaaatactgc tcaaaacgct tctcttcgta
    66781 agcatcaagc caggtaaaat cgggctggtt atcggcaaaa ataccggtca tcagttcgat
    66841 atacgggccg ttattgtcgg tcagactctt atcccacgcc tggccaaatt cactgtgtcc
    66901 ccaactccac tgttttttac ctggcgcaat atggtggttg gcaacgtgca gcaaaccgcc
    66961 atcttcatcg tgacaccacg cgccgacaaa atcgtactgc gatttttcgg ccatatatga
    67021 ggttggaacg ggcacatttt tatagcgaga aatgtccact ccagcggagt agtccacttt
    67081 gtagtaagtg ccggtagcga tggggaaagc ggagacggcc cgtttgccgt gatcaaacac
    67141 cgcagtcaaa tccggtggga aaacgctctg atgcccttca ccccctttca ctgccgggtt
    67201 ggcccaccac aagaaatgac gcggcgtggc gttgccgtta tagacgcggc tggcgatttc
    67261 cagcgccgcc cagggcgcag ggtgaaacct gtcatcacct gtaaaccatg catcggctcc
    67321 gtttcgccta cccacaccgt ctgtgcaccg tcttcatggg cttcgagggt gaaatcaacg
    67381 ggcataaagg tggtcgggcg atgatgttgc ggccagttaa actcaatccc accggaaatc
    67441 cacggcccca gcagccccac cagcgcaggt ttaatgactt cattgtgata aacaaaatcg
    67501 cgctgtttca ctttatccca cgcgcgatgc acccgaccgc ccagttccgg caggatcatc
    67561 actttgatgt agtcgttttc cagccacacc gcctgccagg atttcagggt tttctgctcg
    67621 ctcagcgtat cggtcacgcc gtagggataa accgcgccgg acgatccctg ataaacgcga
    67681 ttttccagga acatgggatg tatatcctgc ggcccggttt cataggtcgg gatctcaacg
    67741 cgctcttgcc acacttttac tggagtcatg gtgccctcaa tattaacaag acatactgaa
    67801 ttaaaagatt tgtggcagtg tattgaacaa tctggcaatg ttttcgcgga ataatcacgc
    67861 aattaactaa acaaggttta gtgaagatga gagcctgcat taacaatcaa cagattcgcc
    67921 accataacaa atgcgtgatt ctggaactgc tgtaccggca aaagcgcgcc aataaatcaa
    67981 cgctggcccg gctggcgcaa atttcgattc cggcagtcag taatattttg caggaactgg
    68041 aaagcgaaaa acgggtggtg aatatcgacg atgaaagcca gacgcgcggg catagtagcg
    68101 gtacatggct gattgcgccg gaaggtgact ggacgctgtg cctgaacgtg acgcccacca
    68161 gtattgagtg tcaggttgct aatgcttgtt taagtccgaa aggtgaattt gagtatttac
    68221 agattgatgc accgacgccg caggcgctgc tgtccgaaat cgaaaaatgc tggcatcgcc
    68281 accgtaaatt gtggccggac cgcaccatca atctggcgct ggcaatccac ggtcaggttg
    68341 atccggtgac cggcgtgtcg caaaccatgc cgcaagcgcc gtggacaacg ccggttgagg
    68401 tgaagtatct gctggaagag aagctcggca ttcgggtgat ggtcgataat gactgcgtga
    68461 tgctggcgct ggcggagaaa tggcaaaata attcgcagaa acgggatttc tgcgtgatca
    68521 acgttgatta cggcattggc tcgtcgttcg tgattaacga gcaaatttat cgcggcagtt
    68581 tgtatggtag cggacagatt ggtcacacca tcgttaatcc ggatggcgtc gtctgcgact
    68641 gtggacgtta cggctgcctg gaaaccgtcg cctcgttaag tgcattaaaa aaacaggcgc
    68701 gggtatggct aaaatcacaa ccggttaata ctcagcttga tccggaaaaa ctgactacag
    68761 cgcagttaat cgctgcctgg tagagtggag aaccgtggat caccagttgg gttgatcgca
    68821 gtgccaatgc cattggtttg agtctgtata acttcctcaa catcctcaat attaatcaga
    68881 tttggttgta cggtcgcagt tgtgcctttg gtgagcactg gcttaatacc attattcgcc
    68941 agacgggatt taacccgttc gaccgcgacg aagggccgag cgtgaaagca acgcaaattg
    69001 gctttgggca attaagccgc gcacaacagg tgttggggat tggctatttg tatgttgagg
    69061 cgcagttacg acagatttga tggcgcaata acgtagaaag gcttcccgaa ggaagccttg
    69121 atgatcacat acctaagatt gcctgatgcg ctacgcttat caggcctaca cggatattgc
    69181 aatatattga atttgcaaag ttttgtaggc cggataaggc gttcacgccg catccggcat
    69241 gaacaacgag cacattgaca gcaaatcacc gtttcgctta tgcgtaaacc gggaaacgtg
    69301 cgcagatgtc gagaacttta cctttgatgc gctcgataac ggcttcatca ttgatgctgt
    69361 ccagcacgtc acacatccag ccagccagtt ctttcgcttc tgcttctttg aagccgcgac
    69421 gcgtaatcgc cggagtaccc acgcgaatac cggaggtcac aaacgggctc ttcggatcgt
    69481 tcggtacgct gtttttgttg acggtgatgt tagcacggcc cagagcggcg tctgcttctt
    69541 taccggtcag gtttttatca accagatcaa ccaggaacag gtggttatca gtgccgccgg
    69601 aaaccacttt gtagccgcgc tcgaggaaca cttctaccat cgctttagcg tttttcgcga
    69661 cctgctgctg gtaagttttg aactcaggct ccatcgcttc tttcagagca accgctttac
    69721 cggcgattac gtgcatcaac ggaccgccct gaccaccagg gaaaacggca gagttcagtt
    69781 ttttgtacag ctcttcgcta ccacctttcg ccaggatcag gccgccgcgc ggacccgcca
    69841 gggttttgtg agtggtggta gtaacaacgt gagcatgagg aaccgggttc gggtagacgc
    69901 cagcagcaac caggcccgca acgtgcgcca tatcaacgaa caggtaagca ccgatgctgt
    69961 cagcgatttc acgcattttc gcccagtcaa ccacgccgga atatgcagag aaaccaccga
    70021 taatcatttt cggcttgtgt tctttggctt gtttttccag atcggcgtag tcgatatgac
    70081 cggtagcatc gataccgtaa ggaacgatgt tgtacagttt accggagaag ttaaccggag
    70141 aaccgtgagt caggtgaccg ccatgcgcca ggttcatacc cagaacggta tcacctggtt
    70201 ccagcagcgc ggtgtagacc gcaaagttag cctgggagcc ggagtgcggc tggacgttag
    70261 cgtagtcagc gccgaacagt tctttcgcac gatcgatcgc cagttgttca acgatatcaa
    70321 catactcgca accgccgtag tagcgtttgc ccggataacc ttcagcatat ttgttggtca
    70381 gctgagaacc ctgcgcctgc attacgcgcg ggctggtgta gttttcggag gcgatcagtt
    70441 cgatgtgctc ttcctgacgt actttttcct gctccatagc ctgccacagt tcggcatcat
    70501 aatcggcaat gttcatttca cgctttaaca tccgcatctc ctgactcagc taacaataaa
    70561 atttttggcc tttataggcg gtcctgttgg acaacggcga acagtataac cgaatcattg
    70621 tgcgataaca ggtcttgaca aaggaattta cgcaaacgat taccttcagg ctacgcaagg
    70681 ctttggagaa taaagagctt gcaaccggaa acggatttct tttcaggttt gtgatgcaaa
    70741 tttttcactt catcacattc tttctgaaaa acaccaaaga accatttaca ttgcagggct
    70801 attttttata agatgcattt gagatacatc aattaagatg caaaaaaagg aagaccatat
    70861 gcttgacgct caaaccatcg ctacagtaaa agccaccatc cctttactgg tggaaacggg
    70921 gccaaagtta accgcccatt tctacgaccg tatgtttact cataacccag aactcaaaga
    70981 aatttttaac atgagtaacc agcgtaatgg cgatcaacgt gaagccctgt ttaacgctat
    71041 tgccgcctac gccagtaata ttgaaaacct gcctgcgctg ctgccagcgg tagaaaaaat
    71101 cgcgcagaag cacaccagct tccagatcaa accggaacag tacaacatcg tcggtgaaca
    71161 cctgttggca acgctggacg aaatgttcag cccggggcag gaagtgctgg acgcgtgggg
    71221 taaagcctat ggtgtactgg ctaatgtatt tatcaatcgc gaggcggaaa tctataacga
    71281 aaacgccagc aaagccggtg gttgggaagg tactcgcgat ttccgcattg tggctaaaac
    71341 accgcgcagc gcgcttatca ccagcttcga actggagccg gtcgacggtg gcgcagtggc
    71401 agaataccgt ccggggcaat atctcggcgt ctggctgaag ccggaaggtt tcccgcatca
    71461 ggaaattcgt cagtactctt tgactcgcaa accggatggc aaaggctatc gtattgcggt
    71521 gaaacgcgaa gagggtgggc aggtatccaa ctggttgcac aatcacgcca atgttggcga
    71581 tgtcgtgaaa ctggtcgctc cggcaggtga tttctttatg gctgtcgcag atgacacacc
    71641 agtgacgtta atctctgccg gtgttggtca aacgccaatg ctggcaatgc tcgacacgct
    71701 ggcaaaagca ggccacacag cacaagtgaa ctggttccat gcggcagaaa atggcgatgt
    71761 tcacgccttt gccgatgaag ttaaggaact ggggcagtca ctgccgcgct ttaccgcgca
    71821 cacctggcat cgtcagccga gcgaagccga tcgcgctaaa ggtcagtttg atagcgaagg
    71881 tctgatggat ttgagcaaac tggaaggtgc gttcagcgat ccgacaatgc agttctatct
    71941 ctgcggcccg gttggcttca tgcagtttac cgcgaaacag ttagtggatc tgggcgtgaa
    72001 gcaggaaaac attcattacg aatgctttgg cccgcataag gtgctgtaat ttgatgttgc
    72061 cggatggaaa catccggcaa cccttgacgc ggtttaaatt gccgcgtcgt cctcttcacc
    72121 ggtacggatg cgaatgaccc gtgccacgtc aaagacgaag attttaccgt caccgatttt
    72181 gccggtttgc gccgtgcgaa taatggtatc gacacaggta tcgacaatgt cgtccggtac
    72241 gacaatctca attttcactt tcggcagaaa atccaccata tactccgcgc cgcggtacag
    72301 ctcggtatgg cctttctggc gaccaaagcc tttcacttcg gtcaccgtca tgccggtaat
    72361 accgacttcg gccagtgctt cgcggacatc gtccagcttg aagggtttta taatcgcatc
    72421 aatctttttc atgctattcc ttgaaaaggt cgcctgtctt ttgatctgct aaacgtaaca
    72481 cataacgcca attcattcct tgaaatcgtt tgcatccagc tcgtgtcggg aaagcagttt
    72541 ataaaattct gtccggttgc gccccgccat tctcgccgcg tgggtgacgt tgcctttggt
    72601 gatttgcagc agcttacgca aatagttgag ttcaaactga ttacgtgcct caacaaaggt
    72661 tggcagcgcc gtattttcac cctccagcgc ctgctccacc agcgcatcac taatcaccgg
    72721 agatgaggtc agcgccacgc actgttcaat cacgttgacc aactggcgca cattacccgg
    72781 ccagctcgcg gtcatcaggc gtttcatcgc atcggtagag aacgcgcgga caaacggttt
    72841 atgtcgctct gccgcctggc gcaacaggtg atttgccagt agcggaatgt cttctgtgcg
    72901 ctccgccagt gccggaattt tcaggctgac aacgttgagg cggtaataga gatcttcgcg
    72961 gaattccccg cgcgccatcg cttttggcag atcacggtga gtggcagaaa taatccgcac
    73021 attgatatca atatcgcggt tactgcccag cgggcgcact ttacgctcct gcaacacgcg
    73081 cagcagtttg acctgtaacg gcgcaggcat atcgccaatt tcatcgagaa atagcgttcc
    73141 gccttccgcc gcctggaata aaccttcgcg attgctgaca gcgccagtaa acgcgccacg
    73201 cgcatgacca aacagctccg actccagcaa ttgctcgggc aatgcgccac agttaatagc
    73261 aataaatggt ttgctgttgc gcgggctggc gttgtggata gcctgggcga aaatctcttt
    73321 cccggtgccg ctctgaccgt taatcaaaac gctgacgtct gattgcgcca ccagccgcgc
    73381 ctgttccagc aaacgcagca tcagcgggct gcgggtgaca attgcctcgc gccagcgttc
    73441 atcggtggct ggcgcggatt gctccagcgc atcgtcaatt gcctgatata gcgcatcttt
    73501 atcgacaggc ttggtgagga aactaaaaac gccctgctgt gttgcagcaa cggcatcggg
    73561 gatagaacca tgcgcagtaa gaataattac tggcattccc ggctgcactt tctggatttc
    73621 agcaaatagc tgcataccgt ccatttcatc catccgcagg tcgctgataa ctaaatctac
    73681 tttttcgcga ttcagtaccc gtaatccttc agcgccactt tccgccgtga ccacactgta
    73741 gccttcgctg gtcaggcgca ggccaagcag tttcagcaat cccggatcgt catcgaccaa
    73801 taataaatgc gcaggtttat ggctcatcag gagtgacctc atgggtggat ggcgcgggct
    73861 tttcactctc gtgcggcgta tccggcgaga aatttccagt cggtttgcgg gtcgagagct
    73921 ggcgttcaat atcggtcagg ttttccagct tgcgggtggt gagttccagt tgctgctgta
    73981 gaacgtgatg ttgctggcgc aatgtatcca gctcgctgtc gctggactgc tggagtttac
    74041 tgtaacgttg gcgctcttcc gccagttgca gttgcagcgc ctgaccgtcg cgccagagtt
    74101 gatacagtgg gcgaacctgt gccgggatct cggtacttaa cgcttcaata cgcgcgacca
    74161 gctggcggcg ctcatacggc gtaattttgg cgtcggcgag caaaatccct tgtttaaagg
    74221 tattttgcca gctgccgtcg tcatattggc gagcttgctg acgcgactgc gcaggcatta
    74281 aacgatcagc acaatccatc gcccgcagcc agtaaagcgg attggtttcg gttgatttac
    74341 cttgcaacgc ccagatgtcg ctacattcag tagaaagata gtcagccagt tgataaaccg
    74401 gaattttttc ttctgctggc gtatcaatgg ctggcttatt gtgattctgc acgcaaccca
    74461 gcaatgccag acatggcagc cctgccagcc acagccgtcg gggcaataat cgttgaaaaa
    74521 tgtgtcgcat attcaccaga cttaaagcct atcccagtgg gcgtaattgt tgcagacagt
    74581 ctggacatgg acagcgcgga gaaaccggag cgtacatatc gtacgtgaag atttcgagca
    74641 ctgcccgggg ccgaactgac aaataaaata gcctgatagg ataggctgtt agcattattt
    74701 cgtgtttttc gacgacggta attcaatgcg gaaacaaacg tcttgcccgc tctcgtcgac
    74761 cagatacagt tccccttgca tacggcgaat acaatccctg gcaatgctta atcccagacc
    74821 gctgcccttc accgcccctt ttcgctggtg gcttccctga aaaaagggtt cgaagatcat
    74881 ggcgcgttcc tcttgcggaa tgggcgtgcc tgtattgatg acatcaatat aaacccgcgc
    74941 accatgtgaa ctgctgcgaa ggcaaatgtt accggattca gccccgtagt gcaccgcatt
    75001 ggagtaaaga ttatccagta cgctcatcag cagcattggc tccgccaggc aagctgttgc
    75061 tttgagatcg acgtcggtat gcatcatttt agcccgtgcg ggcaggctat gagcagaaac
    75121 cactgtctcc accagcggtg ctaattcaac attctccagt tccaccgcac tgtccgcctg
    75181 tttacggttg taatcaagca gttgttcgat cagtttttgc aaattgcggc tgctgctatc
    75241 aagaatgctc accacctctt tttgctctgg cgtaagcggc ccgacaacct ggtcagccag
    75301 taattcagtg ccctcgcgca tactcgccag tggcgttttt aattcatgag ataaatgtct
    75361 taaaaattga tggcgttggg attccagcca tgacaggcgc tcacttaacc aaagaatacg
    75421 ttgcccaacc gagcgtaact cgctcggtcc actgaacgaa acgctattgc ccagagaacg
    75481 cccttccccc agacggttga tcatgcgctc gatatttttc accggcccga taatcatccg
    75541 cgtgaaaagt agcaccatca ccagactcac cagaaatagc accagcgatt gccaaccaaa
    75601 atattgccca cgttcggcga tttcacgctg aagttgctgc ccacgagaga acaccactgt
    75661 gcgcgtggcc tgcaccattt cggtattggc actggcaaag gcttcaagac gcgcggcggc
    75721 ggcagcatcg ggaccactgt tgttacactg aagttgggcc agattgttca aatcctgacg
    75781 caatgcctgg taaagtttat cgtctggcag tacgcctgcg tgggcatcga gcatttcgct
    75841 gtaacgcttg cgctggcttt gataaacctt cgcaagcgta gggtcgtcca gcacgcaata
    75901 ctgacggtag ctacgctcca tctccagcgc cgcgttggtc atcgcttcac tacgccgggc
    75961 atcaataagc gtagtgcggt taaccagcgc cgcctgatcg ctaagcgcat tcaggctttg
    76021 ccaggcttgc catgccagca ccaacagggg tagtagaatt agcaaaaatg ccagcattac
    76081 cagttgtcgt aatgagcggg gaaaaacggg ccagcgtttc aaggtgttac tctcgtcaga
    76141 cgcgaatagc ctgatgctaa ccgaggggaa gttcagatac aacaaagccg ggaattaccc
    76201 ggctttgtta tggaataagg cggtgcctaa ctcgacgttt cgcccgatgg ttgatatagc
    76261 tacgctgata tcagaagttg gacggcaggc accttgttgt gcgtcattcg aattttatgt
    76321 agcacgtccc gaaggggctg acataagtcg gtgaatgagc cactgcttac tattatgcag
    76381 taactgtgcc aataaagaaa atagtttggt aacgtactga ttatacgttg ctttgaggga
    76441 tttatggctt cttcgctatt tgaatgtaaa tcagccactg tgtcgctaaa aagagacaac
    76501 ttaagataaa cttattagat tacaataaaa tcaatcattt aagtgtcgcc agaaagcgac
    76561 acggtaaacc acccattgtc gtgatttaca gacacaaaaa agctcccgga gttgggagct
    76621 tatgatagtg gttggtgctt aatgccgcat ccgggctgca aaaccaatga actgatgact
    76681 taccccaact gcttacgcgc attgcggaaa atgcgcatcc atgggccatc ctcgccccag
    76741 ttttccggat gccaggagtt gctgacagta cggaaaacac gttccgggtg cggcatcata
    76801 atggtgactc gaccactttc agtcgtaacc gccgtaatac cgttcggtga accgttcggg
    76861 ttagccgggt aggtttcagt gactttgccg aagttatcga catagcgcag tgccaccagc
    76921 cctttgcttt ccagtgccgc cagatgcgcc gcatcacgca cttccacgcg cccttcacca
    76981 tgagagacag caatcggcat ttgcgagccc accatcccct gcaacagcag agacgggctt
    77041 tgggttactt caaccaggct gaaacgcgct tcaaagcgat cggaggtgtt acgcacaaaa
    77101 cgtggccaca actcgctccc cgggatcagt tcacgcaggt tagacatcat ctggcaaccg
    77161 ttacataccc ccagcgccag cgtttgcgga cggtggaaga aggttgcaaa ctcatcgcgt
    77221 acacggtcat tgaacaggat tgacttcgcc caaccttcac cggcacccag cacatcaccg
    77281 taggagaaac caccgcacgc gaccagggcg tggaaatctt ccaggcccgt gcgtccggtc
    77341 agcaggtcac tcatatgcac gtcgatagca tcaaagcctg cacggtggaa agctgccgcc
    77401 atttcaacat gcgagttcac gccctgctca cgcagcacag caactttcgg acgtgcgcca
    77461 gtggcaatat acggtgctgc cacatcttcg ttgatatcga acgacagttt cacattcagg
    77521 cccggatcgg cgtcgtttga tttcgcctgg tgctcctggt cagcacactc cgggttgtca
    77581 cgcaggcgct gcatctgcca ggtagtttct gcccaccaga cacgcaacgt ggtgcggctt
    77641 tcgctgaata cagtctgccc gttggcggta atcacaaaac ggtcaccgga aaccgcctgc
    77701 cctacataat ggacacaatc agcaagccca tgctgtgcca gtacggactc gaccgcttca
    77761 cggtcagcgg cacgaacctg aatcaccgca cccagttctt cgttaaacaa cgccgccagg
    77821 cgatcgtcgc ccagagaggc aatatccgcg ttaatgccgc aatgaccagc aaaggccatt
    77881 tccgccagcg ttaccagcag gccgccatca gagcggtcgt ggtatgcgag cagcttacgc
    77941 tgtgcaacca gcgcctgaat cgcgtcatag aagcctttca gttgcgcgac gtcgcgtaca
    78001 tctgccggtt tgtcgccaag ctgacgataa acctgcgcca gcgccgttgc gcccagcgcg
    78061 ttattgcctt tgcccaaatc aatcagcagc agtgtgttat cttcggtaga aagctgtggc
    78121 gtgatggtgt gacgcacatc ttccacgcgg gcaaatgcag aaatcaccag cgacagcggc
    78181 gacgtcattt cgcgctcttc gttaccttcc tgccagcggg ttttcatcga catagagtct
    78241 ttacccaccg ggatcgtcag gcccagcgcc ggacaaagct cttcgcccac ggctttaacg
    78301 gcttcataca ggcccgcatc ttcaccaggg tggcctgccg ccgccatcca gttggcggaa
    78361 agtttaatgc gtttgatatc gccaatctgt gttgcggcaa tattggttaa cgcttcaccg
    78421 accgccagac gggcagaggc ggcgaaatcc agcagtgcaa ccggcgcacg ctcgccaatc
    78481 gccatcgctt caccgtagta gctgtcgagg ctggcggtag tgaccgcgca gttagcgacc
    78541 ggcacctgcc acggccccac catctgatcg cgcgctacca tgccggttac gctgcggtcg
    78601 ccgatggtca ccaggaaggt tttctctgcc acggtcggca gatgcagtac gcgtttcacc
    78661 gcgtctgcaa tggtgatccc ttcacggacc agcgcatcgc ccttcgcttt cagcgtttgt
    78721 acatcgcggg tcatcttcgg cgttttacca agcaggacgt ccagcggcag atcgatcggc
    78781 tgattatcaa aatgacgatc gtgcagagaa agatgcagtt cttcggtcgc ttcaccaatc
    78841 accgcgtagg gtgcgcgctc acgcttacac agttcgtcaa acagcggtaa ttgatcggca
    78901 gcaaccgcca gcacgtagcg ttcctgggat tcgttacacc agatttccag cgggctcatg
    78961 cccggttcgt cgcttagaat atcgcgcagt tcaaatttac cgccgcgccc gccgtcgctc
    79021 accagttccg gcatcgcgtt agaaagaccg ccagcgccga cgtcgtggat aaacaggatt
    79081 gggttggcat caccaagctg ccagcaacgg tcgatcactt cctggcagcg acgctccatc
    79141 tccgggttgt cgcgctgtac ggaagcaaag tcgaggtcgg catcagactg acctgacgcc
    79201 atggaagacg ccgctccgcc gccaagaccg atgttcattg ccgggccgcc gagaacgacc
    79261 agcttcgcac cgacgttgat ctcgcctttt tgtacgtgat cggcgcgaat gttgccgatc
    79321 ccgcccgcca gcatgatcgg tttgtgataa ccgcgcagct cttcgccgtt gtggctgttc
    79381 actttttctt cataagtacg gaagtagcca ttcagtgccg gacgaccaaa ttcgttgtta
    79441 aacgccgcgc cgcccagcgg gccttcggtc atgatgtcca gcgcggtgac aatgcgctca
    79501 ggcttaccga aatcttcttc ccacggctgt tcgaagccag gaattcgcag gttggaaacg
    79561 gagaaaccaa ccagaccagc cttcggcttc gcaccgcgtc cggtggcgcc ttcatcacga
    79621 atttcgccgc cggaacccgt cgccgcgccc ggccacggag aaatcgccgt cgggtggttg
    79681 tgagtttcga ctttcatcag aatatgcgcc ggttcctgat ggaaatcgta gcggcccgtt
    79741 tcgtggtcag caaagtagcg gcccacttca gaaccttcca ttacggcggc gttatcttta
    79801 taagcagaga gaacgtgatc tggcgtggtt tcgaaagtat ttttgatcat cttgaacagc
    79861 gatttcggct gctgttcacc gtcgataatc cagtcggcat taaaaatttt gtggcggcag
    79921 tgctcagagt tcgcctgagc gaacatatac agctcgatgt cgttcgggtt acgaccaagc
    79981 tttgtgaaag catcctgcag atagtcaatt tcatcttccg ccagagccag gccaagacgc
    80041 aggttagcgt cgatcagcgc ctgacggccc tgccccagca aatcaacgct ggtaaccgga
    80101 gtcggttgat ggtgggcaaa caactgctct gcatcatcta aagcaaaaaa gaccgtttcc
    80161 atcatgcggt cgtgcagttc agcggtaacc tgctgccatt gttcattggt cagcgtaccg
    80221 gcttctatat agtaagcaac gccgcgctca aggcggttta cctgttgtag cccgcagtta
    80281 tgggcaatat cggtcgcttt cgaagaccag ggagagatgg tgccaggacg cggggtcacc
    80341 agcaggagtt tgccttgcgg ggcgtggctg gcgagtgccg ggccatattt cagcaggcgt
    80401 tcaagttgtg cgtgctcatc atcgtttaac ggcgcattga ggtcagcaaa atggacatac
    80461 tcggcgtaaa tattgtgaac cgggagcctg gcagcctgaa aacgtgccag cagtttgttg
    80521 attcggaatg ccgacagtgc aggcgaacca cgcagaattt ccatcataag tctctcgtct
    80581 tctaagcttt cggtgtaccc aaggggggga aacgggcgtc attataaaga atctgatgcg
    80641 ctgacgaaac cgtttgcgtg gaaataaaat caccatcgtg aattagcaac gcgtgccgcc
    80701 aatggctgta ataagttgcc atctggcgca ggtttacgca aaatgccgct catttatgag
    80761 taaacctttc actattatta cgttttttca agctgggacg cgcacgacac agagaattaa
    80821 ctaattgaaa aaattaaaga ttaattatct gttcatcggc attctggcac tgctgctcgc
    80881 ggtcgctctc tggccatcca ttccctggtt tggtaaagcc gacaaccgta tcgccgccat
    80941 tcaagcgcgg ggagagttgc gtgtgagcac cattcatact cccctgactt ataacgaaat
    81001 caacgggaaa ccttttggcc tggattacga actggcgaaa cagtttgccg attacctcgg
    81061 tgtaaaactg aaagtgaccg tgcggcagaa tatcagccag ctgtttgacg acctcgataa
    81121 tggtaacgcc gacctgctgg cggcaggact ggtctataac agtgagcggg taaaaaatta
    81181 tcagcctggc cctacctatt attccgtgtc acaacaactg gtttataaag tgagtcagta
    81241 tcgcccgcgc acactgggca acctgacggc ggagcaactc accgttgcac cgggtcacgt
    81301 ggtggttaac gatctccaaa ccctgaaaga cacaaaattc ccggaattaa gctggaaggt
    81361 agacgacaaa aaaggctctg cggaattaat ggaagatgtc atcgaaggaa aactcgatta
    81421 caccattgct gattctgtcg ccatcagcct gtttcagcgc gttcacccgg agctcgccgt
    81481 agcgctcgat atcaccgatg aacaaccggt gacttggttt agcccgttag atggcgataa
    81541 taccctttcc gccgccctgc tcgacttctt caacgaaatg aatgaagacg gtacgctggc
    81601 acgcattgaa gagaaatacc tggggcatgg cgatgatttt gattacgtcg atacgcgcac
    81661 atttttacgc gccgtcgatg cggtactgcc gcagttaaag cccctgtttg agaaatacgc
    81721 cgaagaaatt gactggcgtt tgctggccgc tattgcttat caggaatcgc actgggatgc
    81781 acaggccact tcaccgacgg gtgtgcgcgg catgatgatg ttaaccaaaa ataccgcgca
    81841 aagcctcggc attacggatc gtaccgatgc cgaacagagc atcagcggtg gcgtgcgtta
    81901 tttgcaggat atgatgagta aagtgccgga aagtgtgccg gagaacgagc ggatctggtt
    81961 tgccctcgct gcgtacaata tgggctatgc gcatatgctg gatgcccgcg ccctgacggc
    82021 aaaaaccaaa gggaatcctg acagttgggc tgacgtaaaa cagcgtctgc ctttacttag
    82081 ccagaaaccc tattacagca agctgactta cggctacgct cgtggacatg aagcctacgc
    82141 ttatgtcgaa aatattcgta agtatcagat tagcctggtg ggttatctgc aagagaaaga
    82201 gaagcaggct acagaagcgg cgatgcaact ggcgcaggat tatccggcgg tatcgcctac
    82261 ggagttgggc aaagagaaat ttccttttct ctcgtttctt tcccagtcgt catcaaacta
    82321 tttgacccat tctccctctc tgctgttttc caggaaaggg agtgaagaga aacaaaatta
    82381 atccgtcgag ggttgcgctt ttttctgcgc tttaatttcc tggcggcgca tgcgaaagaa
    82441 gtcactgagc aacgccgcgc actcatccgc cagtattcct tccgtaattt ccactcggtg
    82501 attcataccc ggatgatgca gcacatccat taaagatccc gcagcgccag ttttcgcgtc
    82561 acgcgcacca aagaccacgc gaccaatgcg actgtggatc atcgctccgg cacacattac
    82621 acatggttca agcgtgacat acaacgtggc gtcgatcaga cgataatttt gcatcaccag
    82681 accaccctgc cgcagggcca tgatttctgc atgtgcggtg ggatcatggc gaccaatcgg
    82741 gcggttccag ccttcgccga ttacccgatt gttatgcact aataccgcgc cgaccggcac
    82801 ttcccgctca tcccaggcac gtttcgccag cgtcagcgcg tgacgcatcc agtattcgtg
    82861 gctaaattcg acttcagaca aaaagaaaac tccggttata aaagcgcggc gcattataca
    82921 cggacgctat gctttactct attccagttg ctggagttca ccgcgcgggg ttactcgcca
    82981 acgatgctga cagaaataaa gcagcggatt gtcctgatta ctgtcgctat agccactgta
    83041 cagccgcagc ggagtgccga ttttgcgctc cagttgtgcg accttttcat gtcccagaca
    83101 acgcatcgtc aatacccaac caccatagcc acgctgaatt tggctggcga taagattaac
    83161 ccgcggcagc cagggcgtat cgaaataaac cgcttcaacc agcggctgcg gagagccggt
    83221 aatcaaccag atatcagcat cggaacttaa caggtaggtg gttaatcgct cctgaaccag
    83281 cggaaaggcg gtaacattgt cgcgaaacca gcgcacgaaa tcggcctgca acgtctgtaa
    83341 acgtgcttcg ctgtgaccaa aagtgcaccc ccacagaagc agactcatcg gccagcgtgc
    83401 cgcacgacct tttatcaata acgcaatggc tataatcggt aacaacggca ggacaagtaa
    83461 cgcattcagc ggttggcgac gcagtaaata gcgcagaaaa ctgccgaaca tatcctgctg
    83521 atgcaatgtt ccatctaagt caaaaaacac cacacgacgc tcgtgagttg ccaaaccata
    83581 ctcctctgga ctgaaacatc ctgattaatt ttcgctacta catagcctaa cagatagatc
    83641 atcacttttc cggcaacaat cgaccgcatc atggctaact ggaattttta attcatggca
    83701 attagcggca atggaatata aaattcactc gcgtgtgtct catattgcct gttgtcgcca
    83761 ctatttcatc gcgaggaaag atatgaacgg cttacttcgt atccgtcagc gttaccaggg
    83821 gcttgcccag agtgataaaa aactggcgga ttatctgctg ctacaacctg atacggcgcg
    83881 ccatttaagc tctcagcaac tggccaacga agccggagtc agtcagtcca gcgtcgtgaa
    83941 gttcgcgcaa aaactcggct ataaaggttt tccggcgctt aagttggcgt tgagtgaagc
    84001 gctggcaagc cagccggaat cgccctccgt gcccattcat aaccaaatcc gcggtgatga
    84061 tccgttacgg ctggtcggcg aaaaactgat taaagaaaat actgccgcta tgtacgcaac
    84121 gctaaacgtt aatagtgaag agaaactgca tgaatgcgta acaatgttgc gctctgcgcg
    84181 gcggataatt ctgaccggta ttggcgcttc gggtctggtg gcgcaaaact ttgcctggaa
    84241 gctgatgaag attggcttca atgctgccgc agtgcgcgat atgcatgcgc tactcgcaac
    84301 agtacaggcg tcgtcccctg acgatctgtt attagccatt tcctacaccg gtgtacgacg
    84361 cgagttaaac ctggcggcag atgagatgct gcgagtgggc ggaaaagtgc tggcgattac
    84421 cggctttact ccgaatgccc tgcaacagcg tgcttctcat tgcctgtata ccattgccga
    84481 agaacaagcg acaaacagtg cttcaatctc tgcttgtcac gctcagggaa tgttaacgga
    84541 tttgctgttc attgcgctga ttcagcagga tctggaactg gcaccagaac gtattcgtca
    84601 tagtgaagcg ctggtgaaaa aactggtctg agtaaagaat gcgcgtacaa tgcccgcccg
    84661 gtttgtgttg ttttgagagt ttccttatgg cgttgttaat cactaaaaaa tgcatcaatt
    84721 gtgatatgtg tgaaatcaaa taaaaacaca tttattttca tagtgataca gcaatgtgtt
    84781 attgaatgtg ttgttggatt tatggtgcaa aaaaattttg cataagttca gcaacacaaa
    84841 gaacaccact ttctttaacg tcctccgcgc cctaaaggac ggggctttac ggcgcaaaag
    84901 gcctgtgaca taccacatta acttttcagt caaccgtagc acgttcttgt atacgccgtg
    84961 tctgcggttt tatataaccc tggctggaaa cctcttatac aaaaaagtcg attcgccaac
    85021 atcatagatg atcgccacct tctggcgaga aactcctgat gcaattagtc gcccggcctg
    85081 cgcccattat tctggtgaaa gtttgggacg gcgaccgcca attcgtccct gtgcgcgagc
    85141 agcttccagt cctgcttttg ttcgttcaac aatcagttca cgttccattt cagccagggc
    85201 acccatcaca tggaagaaaa agcgtcccat tggtgtgctg gtatcaattg aatcagtcag
    85261 actacgaaag ttgatgcctc gttcgcgcaa ctcctccacc agcacgacaa gatgccgcat
    85321 actgcgcccc agccgatcca gcttccagac aaccagtgtg tcgcctgccg ataatgtcct
    85381 gagcagtttt ttcagccccg gcctttcgga ctttgtgcca cttatcttgt cttcaaaaat
    85441 cagctcgcat cctgcacagt tcaacgcatt acgttgtaga tcggtattct ggtcatttgt
    85501 tgatacgcgt acatagccaa taagcatggt aaatccccct gttaacaaga cactgcgtca
    85561 aaagtcgggg gggagaattg ggtgatctcc ttccatggga atacacagtc attataaa
//
LOCUS       DAKISA010000002        82387 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.002, whole genome shotgun sequence.
ACCESSION   DAKISA010000002 DAKISA010000000
VERSION     DAKISA010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 82387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82387
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.002"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            376..825
                     /gene="alaE"
                     /locus_tag="OVW45_000073"
     CDS             376..825
                     /gene="alaE"
                     /locus_tag="OVW45_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCV5524628.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSHYQQVKA"
     gene            complement(862..1206)
                     /locus_tag="OVW45_000074"
     CDS             complement(862..1206)
                     /locus_tag="OVW45_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCV5524629.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1358..1687
                     /locus_tag="OVW45_000075"
     CDS             1358..1687
                     /locus_tag="OVW45_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCV5524630.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            1935..2180
                     /gene="nrdH"
                     /locus_tag="OVW45_000076"
     CDS             1935..2180
                     /gene="nrdH"
                     /locus_tag="OVW45_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCV5524631.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            2177..2587
                     /gene="nrdI"
                     /locus_tag="OVW45_000077"
                     /pseudo
     CDS             2177..2587
                     /gene="nrdI"
                     /locus_tag="OVW45_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
     gene            2560..4704
                     /gene="nrdE"
                     /locus_tag="OVW45_000078"
     CDS             2560..4704
                     /gene="nrdE"
                     /locus_tag="OVW45_000078"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCV5524632.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            4714..5673
                     /gene="nrdF"
                     /locus_tag="OVW45_000079"
     CDS             4714..5673
                     /gene="nrdF"
                     /locus_tag="OVW45_000079"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCV5524633.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHYFFSGSGSSYVMGKAVETEDEDWNF"
     gene            6029..7231
                     /gene="proV"
                     /locus_tag="OVW45_000080"
     CDS             6029..7231
                     /gene="proV"
                     /locus_tag="OVW45_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCV5524634.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAKLRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            7224..8288
                     /gene="proW"
                     /locus_tag="OVW45_000081"
     CDS             7224..8288
                     /gene="proW"
                     /locus_tag="OVW45_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCV5524635.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNSFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            8346..9338
                     /gene="proX"
                     /locus_tag="OVW45_000082"
     CDS             8346..9338
                     /gene="proX"
                     /locus_tag="OVW45_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCV5524636.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYCEGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            9629..10813
                     /locus_tag="OVW45_000083"
     CDS             9629..10813
                     /locus_tag="OVW45_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCV5524637.1"
                     /translation="MTKPNHELSPALIVLMSIATGQAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            10937..11674
                     /gene="ygaZ"
                     /locus_tag="OVW45_000084"
     CDS             10937..11674
                     /gene="ygaZ"
                     /locus_tag="OVW45_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCV5524638.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            11664..11999
                     /gene="ygaH"
                     /locus_tag="OVW45_000085"
     CDS             11664..11999
                     /gene="ygaH"
                     /locus_tag="OVW45_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCV5524639.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            12090..12620
                     /gene="emrR"
                     /locus_tag="OVW45_000086"
     CDS             12090..12620
                     /gene="emrR"
                     /locus_tag="OVW45_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCV5524640.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            12747..13919
                     /gene="emrA"
                     /locus_tag="OVW45_000087"
     CDS             12747..13919
                     /gene="emrA"
                     /locus_tag="OVW45_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HCV5524641.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            13936..15474
                     /gene="emrB"
                     /locus_tag="OVW45_000088"
     CDS             13936..15474
                     /gene="emrB"
                     /locus_tag="OVW45_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCV5524642.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(15538..16053)
                     /gene="luxS"
                     /locus_tag="OVW45_000089"
     CDS             complement(15538..16053)
                     /gene="luxS"
                     /locus_tag="OVW45_000089"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCV5524643.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(16203..17759)
                     /gene="gshA"
                     /locus_tag="OVW45_000090"
     CDS             complement(16203..17759)
                     /gene="gshA"
                     /locus_tag="OVW45_000090"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCV5524644.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGDAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHT"
     gene            complement(17832..18260)
                     /locus_tag="OVW45_000091"
     CDS             complement(17832..18260)
                     /locus_tag="OVW45_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCV5524645.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKAIGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(18257..18823)
                     /gene="yqaB"
                     /locus_tag="OVW45_000092"
     CDS             complement(18257..18823)
                     /gene="yqaB"
                     /locus_tag="OVW45_000092"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCV5524646.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVDVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRHYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(19104..19180)
                     /locus_tag="OVW45_000093"
     tRNA            complement(19104..19180)
                     /locus_tag="OVW45_000093"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19144..19146),aa:Arg,seq:acg)
     gene            complement(19379..19455)
                     /locus_tag="OVW45_000094"
     tRNA            complement(19379..19455)
                     /locus_tag="OVW45_000094"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19419..19421),aa:Arg,seq:acg)
     gene            complement(19519..19595)
                     /locus_tag="OVW45_000095"
     tRNA            complement(19519..19595)
                     /locus_tag="OVW45_000095"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19559..19561),aa:Arg,seq:acg)
     gene            complement(19599..19691)
                     /locus_tag="OVW45_000096"
     tRNA            complement(19599..19691)
                     /locus_tag="OVW45_000096"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19655..19657),aa:Ser,seq:gct)
     gene            complement(20007..20192)
                     /gene="csrA"
                     /locus_tag="OVW45_000097"
     CDS             complement(20007..20192)
                     /gene="csrA"
                     /locus_tag="OVW45_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCV5524647.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(20427..23057)
                     /gene="alaS"
                     /locus_tag="OVW45_000098"
     CDS             complement(20427..23057)
                     /gene="alaS"
                     /locus_tag="OVW45_000098"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCV5524648.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATEL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(23185..23685)
                     /gene="recX"
                     /locus_tag="OVW45_000099"
     CDS             complement(23185..23685)
                     /gene="recX"
                     /locus_tag="OVW45_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCV5524649.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALVRDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(23913..24974)
                     /gene="recA"
                     /locus_tag="OVW45_000100"
     CDS             complement(23913..24974)
                     /gene="recA"
                     /locus_tag="OVW45_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCV5524650.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(25054..25551)
                     /gene="pncC"
                     /locus_tag="OVW45_000101"
     CDS             complement(25054..25551)
                     /gene="pncC"
                     /locus_tag="OVW45_000101"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCV5524651.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQRGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(25696..26781)
                     /gene="mltB"
                     /locus_tag="OVW45_000102"
     CDS             complement(25696..26781)
                     /gene="mltB"
                     /locus_tag="OVW45_000102"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCV5524652.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            27037..27600
                     /gene="srlA"
                     /locus_tag="OVW45_000103"
                     /gene_synonym="gutA"
     CDS             27037..27600
                     /gene="srlA"
                     /locus_tag="OVW45_000103"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCV5524653.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            27597..28556
                     /gene="srlE"
                     /locus_tag="OVW45_000104"
                     /gene_synonym="gutE"
     CDS             27597..28556
                     /gene="srlE"
                     /locus_tag="OVW45_000104"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCV5524654.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSSPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSHFLTGAPTVLIAWFVSGFIYQ"
     gene            28567..28938
                     /gene="srlB"
                     /locus_tag="OVW45_000105"
                     /gene_synonym="gutB"
     CDS             28567..28938
                     /gene="srlB"
                     /locus_tag="OVW45_000105"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCV5524655.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            28942..29710
                     /gene="srlD"
                     /locus_tag="OVW45_000106"
                     /pseudo
     CDS             28942..29710
                     /gene="srlD"
                     /locus_tag="OVW45_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
     gene            29816..30175
                     /gene="gutM"
                     /locus_tag="OVW45_000107"
     CDS             29816..30175
                     /gene="gutM"
                     /locus_tag="OVW45_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCV5524656.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            30242..31015
                     /gene="srlR"
                     /locus_tag="OVW45_000108"
                     /gene_synonym="gutR"
     CDS             30242..31015
                     /gene="srlR"
                     /locus_tag="OVW45_000108"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCV5524657.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            31008..31973
                     /gene="gutQ"
                     /locus_tag="OVW45_000109"
     CDS             31008..31973
                     /gene="gutQ"
                     /locus_tag="OVW45_000109"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCV5524658.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTSAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(31970..33484)
                     /gene="norR"
                     /locus_tag="OVW45_000110"
     CDS             complement(31970..33484)
                     /gene="norR"
                     /locus_tag="OVW45_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCV5524659.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VMAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            33671..35110
                     /gene="norV"
                     /locus_tag="OVW45_000111"
     CDS             33671..35110
                     /gene="norV"
                     /locus_tag="OVW45_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCV5524660.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTKSDLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            35107..36240
                     /gene="norW"
                     /locus_tag="OVW45_000112"
     CDS             35107..36240
                     /gene="norW"
                     /locus_tag="OVW45_000112"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCV5524661.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLHLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNDDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(36368..38620)
                     /gene="hypF"
                     /locus_tag="OVW45_000113"
     CDS             complement(36368..38620)
                     /gene="hypF"
                     /locus_tag="OVW45_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCV5524662.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETLLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(38773..39300)
                     /gene="hydN"
                     /locus_tag="OVW45_000114"
     CDS             complement(38773..39300)
                     /gene="hydN"
                     /locus_tag="OVW45_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCV5524663.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(39448..40458)
                     /gene="ascG"
                     /locus_tag="OVW45_000115"
     CDS             complement(39448..40458)
                     /gene="ascG"
                     /locus_tag="OVW45_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCV5524664.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            40718..42175
                     /gene="ascF"
                     /locus_tag="OVW45_000116"
     CDS             40718..42175
                     /gene="ascF"
                     /locus_tag="OVW45_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HCV5524665.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSVQPTVAKEVSLN"
     gene            42184..43608
                     /gene="ascB"
                     /locus_tag="OVW45_000117"
     CDS             42184..43608
                     /gene="ascB"
                     /locus_tag="OVW45_000117"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HCV5524666.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(43722..44141)
                     /locus_tag="OVW45_000118"
     CDS             complement(43722..44141)
                     /locus_tag="OVW45_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCV5524667.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(44138..44449)
                     /locus_tag="OVW45_000119"
     CDS             complement(44138..44449)
                     /locus_tag="OVW45_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HCV5524668.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(44611..45384)
                     /locus_tag="OVW45_000120"
     CDS             complement(44611..45384)
                     /locus_tag="OVW45_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5524669.1"
                     /translation="MNIRTGMCAFLLSLVLPAQATSFTEYLPMSDSEYVQKRALKPLL
                     TMPYDAEQTWHFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGMLANIAGKG
                     QLYRADLDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTG
                     VDDLLDLQGNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYT
                     PNRKLVLKPIAGRDPQTEDLAQTQRCLIKGDVLDGVNQN"
     gene            complement(45409..45879)
                     /gene="hycI"
                     /locus_tag="OVW45_000121"
     CDS             complement(45409..45879)
                     /gene="hycI"
                     /locus_tag="OVW45_000121"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HCV5524670.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(45872..46282)
                     /gene="hycH"
                     /locus_tag="OVW45_000122"
     CDS             complement(45872..46282)
                     /gene="hycH"
                     /locus_tag="OVW45_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HCV5524671.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(46279..47046)
                     /gene="hycG"
                     /locus_tag="OVW45_000123"
     CDS             complement(46279..47046)
                     /gene="hycG"
                     /locus_tag="OVW45_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HCV5524672.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQQAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(47046..47588)
                     /gene="hycF"
                     /locus_tag="OVW45_000124"
     CDS             complement(47046..47588)
                     /gene="hycF"
                     /locus_tag="OVW45_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HCV5524673.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(47598..49307)
                     /gene="hycE"
                     /locus_tag="OVW45_000125"
     CDS             complement(47598..49307)
                     /gene="hycE"
                     /locus_tag="OVW45_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCV5524674.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHLHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEMARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(49325..50248)
                     /gene="hycD"
                     /locus_tag="OVW45_000126"
     CDS             complement(49325..50248)
                     /gene="hycD"
                     /locus_tag="OVW45_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCV5524675.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSILLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWTGFGFAFLAFVSLLAA"
     gene            complement(50251..51927)
                     /locus_tag="OVW45_000127"
     CDS             complement(50251..51927)
                     /locus_tag="OVW45_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCV5524676.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGLGAGVTGIALEQPALIALGLVGGL
                     YHLLNHSLFKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPP
                     LNGFAGEWVIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGA
                     PRTKEAENATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQ
                     PMITLLLIACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQ
                     AFAPVLKLRKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(51924..52535)
                     /gene="hycB"
                     /locus_tag="OVW45_000128"
     CDS             complement(51924..52535)
                     /gene="hycB"
                     /locus_tag="OVW45_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCV5524677.1"
                     /translation="MNRFVIVDSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRIDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(52660..53121)
                     /gene="hycA"
                     /locus_tag="OVW45_000129"
     CDS             complement(52660..53121)
                     /gene="hycA"
                     /locus_tag="OVW45_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCV5524678.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            53333..53683
                     /gene="hypA"
                     /locus_tag="OVW45_000130"
     CDS             53333..53683
                     /gene="hypA"
                     /locus_tag="OVW45_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCV5524679.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            53687..54559
                     /gene="hypB"
                     /locus_tag="OVW45_000131"
     CDS             53687..54559
                     /gene="hypB"
                     /locus_tag="OVW45_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCV5524680.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            54550..54822
                     /gene="hypC"
                     /locus_tag="OVW45_000132"
     CDS             54550..54822
                     /gene="hypC"
                     /locus_tag="OVW45_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCV5524681.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            54822..55943
                     /gene="hypD"
                     /locus_tag="OVW45_000133"
     CDS             54822..55943
                     /gene="hypD"
                     /locus_tag="OVW45_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCV5524682.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQENEA"
     gene            55940..56950
                     /gene="hypE"
                     /locus_tag="OVW45_000134"
     CDS             55940..56950
                     /gene="hypE"
                     /locus_tag="OVW45_000134"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCV5524683.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEVALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            57024..59102
                     /gene="flhA"
                     /locus_tag="OVW45_000135"
                     /pseudo
     CDS             57024..59102
                     /gene="flhA"
                     /locus_tag="OVW45_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(59139..59492)
                     /locus_tag="OVW45_000136"
     CDS             complement(59139..59492)
                     /locus_tag="OVW45_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCV5524684.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(59569..59703)
                     /locus_tag="OVW45_000137"
     CDS             complement(59569..59703)
                     /locus_tag="OVW45_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5524685.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            59779..62340
                     /gene="mutS"
                     /locus_tag="OVW45_000138"
     CDS             59779..62340
                     /gene="mutS"
                     /locus_tag="OVW45_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCV5524686.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            62446..63102
                     /gene="pphB"
                     /locus_tag="OVW45_000139"
                     /pseudo
     CDS             62446..63102
                     /gene="pphB"
                     /locus_tag="OVW45_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417214.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(63153..63920)
                     /locus_tag="OVW45_000140"
     CDS             complement(63153..63920)
                     /locus_tag="OVW45_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCV5524687.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            64116..65024
                     /locus_tag="OVW45_000141"
     CDS             64116..65024
                     /locus_tag="OVW45_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCV5524688.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            65021..66283
                     /locus_tag="OVW45_000142"
     CDS             65021..66283
                     /locus_tag="OVW45_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HCV5524689.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALSVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            66280..66918
                     /locus_tag="OVW45_000143"
     CDS             66280..66918
                     /locus_tag="OVW45_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCV5524690.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            66923..67699
                     /locus_tag="OVW45_000144"
     CDS             66923..67699
                     /locus_tag="OVW45_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCV5524691.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            67788..69152
                     /locus_tag="OVW45_000145"
     CDS             67788..69152
                     /locus_tag="OVW45_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HCV5524692.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKHQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(69246..70238)
                     /gene="rpoS"
                     /locus_tag="OVW45_000146"
     CDS             complement(69246..70238)
                     /gene="rpoS"
                     /locus_tag="OVW45_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCV5524693.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(70301..71440)
                     /gene="nlpD"
                     /locus_tag="OVW45_000147"
     CDS             complement(70301..71440)
                     /gene="nlpD"
                     /locus_tag="OVW45_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCV5524694.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(71580..72206)
                     /gene="pcm"
                     /locus_tag="OVW45_000148"
     CDS             complement(71580..72206)
                     /gene="pcm"
                     /locus_tag="OVW45_000148"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCV5524695.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(72200..72961)
                     /gene="surE"
                     /locus_tag="OVW45_000149"
     CDS             complement(72200..72961)
                     /gene="surE"
                     /locus_tag="OVW45_000149"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCV5524696.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(72942..73991)
                     /gene="truD"
                     /locus_tag="OVW45_000150"
     CDS             complement(72942..73991)
                     /gene="truD"
                     /locus_tag="OVW45_000150"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCV5524697.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEK
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(73988..74467)
                     /gene="ispF"
                     /locus_tag="OVW45_000151"
     CDS             complement(73988..74467)
                     /gene="ispF"
                     /locus_tag="OVW45_000151"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCV5524698.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(74467..75177)
                     /gene="ispD"
                     /locus_tag="OVW45_000152"
     CDS             complement(74467..75177)
                     /gene="ispD"
                     /locus_tag="OVW45_000152"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCV5524699.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(75196..75507)
                     /gene="ftsB"
                     /locus_tag="OVW45_000153"
     CDS             complement(75196..75507)
                     /gene="ftsB"
                     /locus_tag="OVW45_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCV5524700.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(75701..76024)
                     /locus_tag="OVW45_000154"
     CDS             complement(75701..76024)
                     /locus_tag="OVW45_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCV5524701.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(76074..76679)
                     /gene="cysC"
                     /locus_tag="OVW45_000155"
     CDS             complement(76074..76679)
                     /gene="cysC"
                     /locus_tag="OVW45_000155"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCV5524702.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(76679..78106)
                     /gene="cysN"
                     /locus_tag="OVW45_000156"
     CDS             complement(76679..78106)
                     /gene="cysN"
                     /locus_tag="OVW45_000156"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCV5524703.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(78108..79016)
                     /gene="cysD"
                     /locus_tag="OVW45_000157"
     CDS             complement(78108..79016)
                     /gene="cysD"
                     /locus_tag="OVW45_000157"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCV5524704.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            79269..80306
                     /gene="iap"
                     /locus_tag="OVW45_000158"
     CDS             79269..80306
                     /gene="iap"
                     /locus_tag="OVW45_000158"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCV5524705.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   80327..80599
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=80388..80414
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaac"
     gene            complement(80696..80989)
                     /gene="cas2"
                     /locus_tag="OVW45_000159"
     CDS             complement(80696..80989)
                     /gene="cas2"
                     /locus_tag="OVW45_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HCV5524706.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(80986..81909)
                     /gene="cas1e"
                     /locus_tag="OVW45_000160"
                     /pseudo
     CDS             complement(80986..81909)
                     /gene="cas1e"
                     /locus_tag="OVW45_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
     gene            complement(81906..>82387)
                     /gene="cas6e"
                     /locus_tag="OVW45_000161"
     CDS             complement(81906..>82387)
                     /gene="cas6e"
                     /locus_tag="OVW45_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HCV5524707.1"
                     /translation="FFVLSQERPAESDIFTIECRPFAPELRTGQSLCFNLRANPTICK
                     AGKRHDLLMEAKRQVRGQVEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQ
                     QLRRENSRQLIQFSSVDYTGMLTVTAPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
BASE COUNT        19512 a        22207 c        21103 g        19565 t
ORIGIN      
        1 actggtgctt tctgctgtta atgtacattt tctggcatca aaaatgctgt aaatgacacg
       61 gtcacgacat acaccgaatt ctctacgctt atagcgttac ctcaccccca aactttcatt
      121 agtgaaatta aaggcaaggt catatctaat tgatttaatt gaaaataaat catatcggca
      181 tgtaaaaatc cacaatgtac aaaaaacgag ccgttacgga atattttatc tacaaaaact
      241 gactaaataa aaatttttca ctaattgaat agtcatagcc agcgatatac gctatgcgaa
      301 aatgcagatg gcaatgagat ccactgcttt catctccatt aacatcccat tacgctttta
      361 ttaaggagca ttagcatgtt ctcaccgcag tcacgcttgc gtcatgcagt tgcagatacg
      421 ttcgcgatgg ttgtttactg ttctgtcgtg aacatgtgta ttgaagtttt cctctccgga
      481 atgagcttcg aacagtcttt ttattccaga ttggtagcga ttccggtgaa catcttaatt
      541 gcatggccat acggtatgta ccgcgatcta tttatgcgcg cggcacgcaa agttagcccg
      601 tcgggctgga taaaaaatct ggctgatatc ctggcttatg tgacgttcca gtcaccggtg
      661 tatgtggcga tcttgttagt ggtgggcgca gactggcatc agattatggc ggcggtcagt
      721 tcaaacatcg ttgtttcgat gttgatgggg gcggtttatg gctacttcct cgattattgc
      781 cgccgactgt ttaaagtcag ccattaccag caggtaaaag cctgactctt cttttcgcga
      841 ctggcatcgc cagtcgcgtc tttaactggc ttcgccaaat aaaccattca aataacgttc
      901 aagcgcaaca cgcgaactga agccatgcgg gataccgtaa tgctcgtgtc gctcgcctgc
      961 taaatagagc ggaaattgtt gataatgatc gcaagtttta atatcggaag cgggttcagc
     1021 cagtgtcgaa ctacgttctt ttaatgttgg atgaattacc agtgcggtac gtcccattcg
     1081 cgcttcgcga ttaacataga cataattttc cccacggcgg taaccataag ctttctgggt
     1141 taccacatcg acagtaaaac cgactttttc aagtacgcgc gccacctcgt ctggtcgtaa
     1201 atacatattt gatcctcgtt attatcaacc gcgggcctac cttacctgat tgcgcattca
     1261 caacgctttc agaaaagtcc ataaagccgc gatgaacctc agttaagaaa attatggtct
     1321 acactgaaaa ttacatcgaa ttctaatggg ggatcatatg tttaaccgac cgaaccgcaa
     1381 cgacgttgat gatggcgtgc aggatatcca gaatgatgtc aatcaattag ctgacagcct
     1441 cgaatctgta ttgaagtcct ggggcagcga cgccaaaggg gaagctgaag ccgcacgcag
     1501 taaagctcag gcattgctga aagaaaccag ggcgcgaatg catggtcgta ctcgcgtcca
     1561 gcaagccgca cgcgatgccg ttggctgcgc ggattctttt gttcgtgaaa gaccctggtg
     1621 tagcgtgggt acagcagctg cggtaggtat ttttatcggc gcactgttaa gcatgcgcaa
     1681 atcgtagtgc aaaaatgata ataaatacgc gtctttaacc ccgaagcctg tcttcggggt
     1741 ttctttttgc ctggtgaatc acaaaaatcc ccctcccccg tcacgctcat atctagggta
     1801 atttcgatca ctatttgcta tatattgtgt ggttgaatct tttttcaact acatctagta
     1861 tctctgtatc aacagagaga caacccgacg cgtatcatcg cgccgtatct tcattttaaa
     1921 cggaaatacg aatcatgcgc ataactattt acactcgtaa cgattgcgtt cagtgccacg
     1981 ccaccaaacg ggcgatggaa aaccggggct ttgattttga aatgattaat gtcgatcgcg
     2041 ttcctgaagc agcggaagcg ttgcgtgctc agggctttcg tcagttgccg gtagtgattg
     2101 ctggcgatct tagctggtct ggtttccgtc cggacatgat taaccgtctg catccagcgc
     2161 cacacgcggc cagtgcatga gccagctcgt ctacttctcc agcagctccg aaaacacgca
     2221 gcgttttatc gaacgtttag gtctgcccgc ggtgcgcatc ccgctcaatg agcgggaacg
     2281 gattcaggta gacgagcctt acatcctgat cgtgccctct tacggcggcg gcggtacggc
     2341 tggcgcggtg ccacgatagg taattcgctt tttaaacgac gagcacaacc gggcgttgct
     2401 tcgcggcgtt attgcttctg gtaatcgcaa ctttggtgag gcgtatggcc gcgccggaga
     2461 tgtgattgcc cggaaatgcg gcgtgccgtg gctgtaccgt tttgaactca tgggtacgca
     2521 aagcgatatc gaaaacgttc gtaaaggagt aaccgaattt tggcaacgac aaccgcagaa
     2581 tgcctgacgc aggaaacgat ggattaccac gcgctgaatg cgatgcttaa cctctacgat
     2641 agcgcaggtc gcattcagtt cgataaagac cgccaggccg ttgacgcctt tatggcgacg
     2701 catgtgcgtc cgaacagtgt gaccttcagt agccagcagc agcgcctgaa ctggctggtc
     2761 aacgaaggtt actatgatga aagcgttctt aatcgctact ctcgcgactt tgtcattacg
     2821 ctgtttgccc acgcacacac cagcggtttt cgtttccaga cattcctcgg ggcatggaag
     2881 ttttacacca gctatacgtt gaagacattc gacggtaaac gttatctgga agattttgcc
     2941 gatcgagtca cgatggtggc gctgacgctg gcacaaggcg atgcgacgct ggcgttgcaa
     3001 ctgaccgatg aaatgctgtc aggacgcttt cagccagcca cgccgacatt cctcaactgc
     3061 ggtaagcagc agcgcggcga actggtttcc tgttttctgc tgcgtattga agacaatatg
     3121 gagtcgattg gccgggcggt aaattccgca ctgcaactgt cgaaacgcgg cggcggcgtg
     3181 gcatttttgc tgtcgaatct gcgagaagcg ggcgcgccaa ttaaacgtat tgaaaatcaa
     3241 tcttctggcg taattccggt gatgaaaatg ctggaagacg cattttccta tgccaaccaa
     3301 ctcggcgcgc gtcagggggc aggtgcagtc tatttacatg ctcatcatcc cgatattctg
     3361 cgttttctcg atacaaaacg ggaaaatgcc gacgaaaaaa tccgcattaa aacactgtcg
     3421 ctgggcgtgg tgatcccgga tatcactttc catctggcaa aagagaatgc gcagatggcg
     3481 ctgttttcgc cctatgacgt tgagcgagtt tatggcaagc cgtttgccga tatcgccatc
     3541 agcgaacact atgacgaact ggttgccgat gaacgcatcc gcaaaaaata cctcaacgcc
     3601 cgtgatttct tccagcggct ggcagaaatc cagtttgagt ccggctatcc ctacatcatg
     3661 tatgaagaca cggtaaaccg tgctaaccct gtcgccgggc gcataaatat gagtaatctc
     3721 tgctcagaga ttttgcaggt caactgtgcc tcagagtatg acgagaatct cgactatgcc
     3781 cgcacaggcc atgatatctc ctgcaattta ggttcgttga atattgcgca caccatggat
     3841 tcccccgatt ttgcccgcac ggtagagact gccgtgcgcg gtttaacggc ggtgtcagat
     3901 atgagtcata tccgcagcgt gccgtccatc gaagccggaa atgccgcctc acacgccatc
     3961 ggactggggc agatgaattt acacggctat ctggcgcgag aaggcatcgc ttatggttcg
     4021 ccggaagcac tggatttcac caatctctat ttctatgcca tcacctggca cgcactgcgt
     4081 acctcgatgt tgctggcgcg cgaacgcggt gaaaccttcg ccgggttcaa acagtcacgt
     4141 tatgccagtg gtgaatattt tagccaatat ctgcaaggta actggcagcc gaaaacggcg
     4201 aaagttggcg aactgtttgc ccgcagcggt attacgttac ctacccgtga gatgtgggcg
     4261 caactgcgcg acgacgtgat gcgctacggc atttacaacc agaatcttca ggcggtgccg
     4321 ccaaccggtt ctatctctta tatcaaccat gctacgtcga gtattcatcc gattgtggcg
     4381 aaagtagaga tacgcaaaga gggcaaaaca ggacgcgttt actaccctgc cccgtttatg
     4441 actaacgaaa atctggcgct gtatcaggat gcttacgaaa tcggcgcaga aaagatcatc
     4501 gacacctacg cggaagcgac tcgccatgtc gatcagggtt tgtcgctgac gctgtttttc
     4561 cccgataccg ccaccactcg cgatatcaac aaagcgcaga tttacgcctg gcgcaaaggt
     4621 atcaaaacgc tctattacat tcgcctgcgt cagatggcgc tggaaggcac tgaaattgaa
     4681 ggctgcgtct cctgtgcact ttaaggaata tctatgaaac tctcacgtat cagcgccatc
     4741 aactggaaca agatatctga cgataaagat ctggaggttt ggaatcgcct gaccagcaat
     4801 ttctggctac cagaaaaggt gccgctgtcg aacgatattc ctgcctggca gacattaact
     4861 gtcgtagaac aacaactgac gatgcgcgtt tttactggcc tgacgctgct cgacacgctg
     4921 caaaatgtta tcggcgcgcc ttcgctgatg cccgatgcac tcacgcctca tgaagaagcg
     4981 gtattatcga atatcagctt tatggaagcg gttcatgccc gctcttacag ttcgattttc
     5041 tcgacgctgt gccagaccaa agatgtcgat gccgcctacg cctggagtga agaaaacgca
     5101 ccgttgcagc gaaaagctca gattattcag caacattatc gcggtgatga tccgctgaaa
     5161 aagaaaatcg ccagtgtgtt tcttgaatct tttttgttct attccggttt ctggctgccg
     5221 atgtattttt ccagccgcgg aaagctgacc aataccgcag acctgatccg tctgattatc
     5281 cgtgatgaag cagtccacgg ttactacata ggctataaat atcagaaaaa catggaaaag
     5341 atatctctgg gacaacgtga agagttgaag agtttcgcct tcgatttgtt gctggaactc
     5401 tacgacaacg agctgcaata caccgatgag ctgtacgccg aaaccccgtg ggctgacgat
     5461 gtgaaagcgt ttctctgtta caacgccaat aaggctttga tgaatctggg ctacgaacct
     5521 ttatttcccg cagaaatggc ggaagtgaat ccggcaatcc tcgccgcgct ttcgccgaat
     5581 gccgatgaaa atcactattt cttttccggt tcaggctcct cttatgtgat ggggaaagcg
     5641 gttgaaacag aagatgaaga ctggaatttc tgagggtgtt attttcaaaa atatcactac
     5701 ccgcgacagg gaaataattc ccgccaaata gctttttatc acgcaaatat tttgtggtga
     5761 tctacactga tactctgttg cattattcgc ctgaaaccac aatattcagg cgttttttcg
     5821 ctatctttga caaaaaatat caacttttct cgatttgctc tcagccctta tatcacggga
     5881 aattccggcg atttgctcgc atcaatattc atgccacatt tgccatcagg ggttgcctca
     5941 gattctcagt atgttagggt agaaaaaagt aactatttcc attgggtaat atatcgacat
     6001 aaacaaataa caggaatctt tctattgcat ggcaattaaa ttagaaatta aaaaccttta
     6061 taaaatattt ggtgagcatc cacagcgagc attcaaatat atcgaacaag gactttcaaa
     6121 agaacaaatt ctggaaaaaa ctgggctatc gcttggcgta aaagacgcca gtctggccat
     6181 tgaagaaggc gagatatttg tcatcatggg attatccggc tcgggtaaat ccacaatggt
     6241 acgccttctc aatcgcctga ttgaacccac ccgcgggcaa gtgctgattg atggtgtgga
     6301 tattgccaaa atatccgatg ccaaactccg tgaggtgcgc agaaaaaaga ttgcgatggt
     6361 cttccagtcc tttgccttaa tgccgcatat gaccgtgctg gacaatactg cgttcggtat
     6421 ggaattggcc ggaattaatg ccgaagaacg ccgggaaaaa gcccttgatg cactgcgtca
     6481 ggtcgggctg gaaaattatg cccacagcta cccggatgaa ctctctggcg ggatgcgtca
     6541 acgtgtggga ttagcccgcg cgttagcgat taatccggat attttattaa tggacgaagc
     6601 cttctcggcg ctcgatccat taattcgcac cgagatgcag gatgagctgg taaaattaca
     6661 ggcgaaacat cagcgcacca ttgtctttat ttcccacgat ctcgatgaag ccatgcgtat
     6721 tggcgaccga attgccatta tgcaaaatgg tgaagtggta caggtcggca caccggatga
     6781 aattctcaat aatccggcga atgattatgt ccgtaccttc ttccgtggcg ttgatattag
     6841 tcaggtattc agtgcgaaag atattgcccg ccggacaccg aatggcttaa ttcgtaaaac
     6901 ccctggcttc ggcccacgtt cggcactgaa attattgcag gatgaagatc gcgaatatgg
     6961 ctacgttatc gaacgcggta ataagtttgt cggcgcagtc tccatcgatt cgcttaaaac
     7021 cgcgttaacg cagcagcaag gtcttgatgc ggcgctgatt gatgcgccgt tagcagtcga
     7081 tgcacaaacg cctcttagcg agttgctctc tcatgtcgga caggcaccct gtgcggtgcc
     7141 cgtggtcgac gaggaccaac agtatgtcgg catcatttcg aaaggaatgc tgctgcgcgc
     7201 tttagatcgt gagggggtaa ataatggctg atcaaaataa tccgtgggat accacgccag
     7261 cggcggacag tgccgcgcaa tccgcagacg cctggggtac accgacgact gcaccgactg
     7321 acggcggtgg tgctgactgg ctgaccagta cgcctgcgcc aaacgtcgag cattttaata
     7381 ttctcgatcc gttccataaa acgctgatcc cgctcgacag ttgggtcact gaagggatcg
     7441 actgggttgt tacccatttc cgtcccgtct tccagggcgt gcgcgttccg gttgattata
     7501 tcctcaacag tttccagcaa ttgctgctgg gtatgccagc gccagtggcg attatcgttt
     7561 tcgctctcat cgcctggcag atttccgggg tcggaatggg cgtggcgacg ctggtttcgc
     7621 tgattgccat cggcgcaatc ggtgcctggt cgcaggcaat ggtgactctg gcgctggtgt
     7681 taaccgccct gctgttctgt atcgtcatcg gtttgccgtt ggggatatgg ctggcgagaa
     7741 gtccgcgagc ggcgaaaatt attcgtccac tgcttgatgc catgcagacc acgccagcgt
     7801 ttgtttatct ggtaccaatc gtcatgctgt ttggtatcgg taacgtgccg ggcgtagtgg
     7861 tgacgatcat ctttgctctg ccgccgatta tccgtctgac gattctgggg attaaccagg
     7921 ttccggcgga tctgattgaa gcctcgcgct cattcggtgc cagcccgcgc cagatgctgt
     7981 tcaaagttca gttaccgctg gcgatgccga ccattatggc gggcgttaac cagacgctga
     8041 tgctggctct ttctatggtg gtcatcgcct cgatgattgc cgtcggcggg ctgggtcaga
     8101 tggtacttcg cggtatcggt cgtctggata tgggccttgc caccgttggc ggcgtcggga
     8161 ttgtgatcct cgccattatc ctcgaccgcc tgacgcaggc cgttgggcgc gactcacgca
     8221 gtcgcggcaa ccgtcgctgg tacaccactg gccctgtcgg tctgctgacc cgcccattca
     8281 ttaagtaact ctgcacttgc ccggtaacgc cgggcattat caccctgcca aaaaaaggaa
     8341 taacaatgcg acatagcgta ctttttgcga cagcgtttgc cacgcttatc tctacacaaa
     8401 cttttgcagc cgatctgccg ggcaaaggca ttactgttaa tccagttcag agcaccatca
     8461 ctgaagaaac cttccagacg ctgctggtca gtcgtgcact ggagaaatta ggttataccg
     8521 tcaataaacc cagcgaagtg gattacaacg ttggctacac ctcacttgct tccggcgatg
     8581 caaccttcac cgccgtgaac tggacgccgc tgcatgacaa catgtacgaa gctgccggtg
     8641 gcgataagaa attttattgt gaaggggtat tcgttaacgg cgcggcacag ggttacctga
     8701 tcgataagaa aaccgccgac cagtacaaaa tcaccaacat cgcacaactg aaagatccga
     8761 agatcgccaa actgttcgat accaacggcg acggtaaagc ggatttaacc ggctgtaacc
     8821 caggctgggg ctgcgaaggt gcgatcaacc accagcttgc cgcgtatgaa ctgaccaaca
     8881 ccgtgacgca taatcagggg aactacgcag cgatgatggc cgacaccatc agtcgctaca
     8941 aagagggcaa accggtgttt tattacacct ggacgccgta ctgggtgagt aatgagctga
     9001 agccagggaa agatgtggtc tggttgcagg tgccgttctc cgcactgccg ggcgataaaa
     9061 acgccgatac caaactgccg aatggtgcga attatggctt cccggtcagc accatgcata
     9121 tcgttgccaa caaagcctgg gccgagaaaa acccggcagc agcgaaactt tttgccatta
     9181 tgcagttgcc agtggcagat attaacgccc agaacgccat tatgcatgac ggcaaagcct
     9241 cagaaggcga tattcagggc catgttgatg gttggatcaa agcccaccag cagcagttcg
     9301 atggctgggt gaatgaggcg ctggcagcgc agaagtaatt tttattcggg cggataagtc
     9361 gtttttgcct catccgccat tctgtgcaca atgcctgatg cgacgctggc gcgtcttatc
     9421 atgcctacaa aatacactca ttccgtatgg cggataaggc gttttcgccg catccgccgt
     9481 tctgtgcaca atgcctgatg cgacgctggc gcgtcttatc atgcctacaa accacatcgc
     9541 acatttcaac aagctatctt tcgcccccat tcatcaacat ccgctattat tgatttccag
     9601 cttaatcatc acctgatgaa caaaaataat gactaaacct aatcatgagc ttagcccggc
     9661 gctgatcgtg ctgatgtcta tcgccaccgg tcaggcggta gccagtaact attacgccca
     9721 gccattgctc gacaccatcg cgcgtaactt ttccctttcc gccagttcgg caggctttat
     9781 tgttaccgcc gcgcagttgg gctatgcagc aggtctgctg tttcttgttc ccctcggtga
     9841 tatgtttgaa cgccgccgcc tgattgtctc gatgacctta ctggcggcag gcggcatgtt
     9901 aatcactgcc agcagtcagt cgctggcgat gatgatcctc ggtacggcat taaccggttt
     9961 attctcagtc gtggcacaaa ttctggttcc gctggcagcg acgctggctt caccggataa
    10021 acgcggcaaa gtggttggca ccattatgag cggtctgctg ttggggatct tgctggcacg
    10081 aaccgttgcc gggttactgg caaacctcgg cggctggcgc accgtctttt gggttgcctc
    10141 ggtgttaatg gcactgatgg cgctagcatt atggcgtggt ctgccacaaa tgaaatcaga
    10201 aacccacctc aactacccac agttgttagg ttccgttttc agcatgttta tcagcgataa
    10261 gatcctccgc acccgcgcat tgctgggctg cctgaccttt gccaacttca gcattctctg
    10321 gacctcaatg gcctttttgc ttgccgctcc accttttaac tacagcgatg gtgtcattgg
    10381 tctgtttgga cttgcgggag ctgccggagc gttgggcgct cgtccggcgg gcggttttgc
    10441 cgataagggt aaatcgcacc acaccacaac tttcggtctg ctgctgctat tactttcatg
    10501 gctggcgatc tggtttggtc acacttccgt actggcgctg attatcggca tcctggtgct
    10561 ggatctcacc gtacagggcg tgcatatcac taaccagacg gtaatttatc gaatacaccc
    10621 tgatgcgcgt aatcgcctga ccgcaggtta catgaccagc tactttattg gcggtgccgc
    10681 cggttcgcta atttcagcct cagcctggca acatggcggt tgggctggcg tttgtctggc
    10741 tggcgcgacg attgccctag ttaacttact ggtctggtgg cgaggttttc atcgtcagga
    10801 agccgcaaat taagcaatca gcaaccgttt tcgtggcttt acacttataa gggtgttaag
    10861 aagcccatca gtctgataag gttaagatat tcattcagtc tatttataat attaacaatc
    10921 gttaagcgta cactctatgg aaagccctac tccacagcct gctcctggtt cggcgacctt
    10981 catggaagga tgcaaagaca gtttaccgat tgttattagt tatattccgg tggcctttgc
    11041 gttcggtctg aatgcgaccc gtctgggatt ctctcctctc gaaagcgttt ttttctcctg
    11101 catcatttat gcaggcgcga gccagttcgt cattaccgcg atgctggcag ccgggagtag
    11161 tttgtgggtt gctgcactga ccgtcatggc aatggatgtt cgccatgtgt tgtatggccc
    11221 gtcactgcgt agccgtatta ttcagcgtct gcaaaaatcg aaaaccgccc tgtgggcgtt
    11281 tggcctgacg gatgaggttt ttgccgccgc aacagcaaaa ctggtacgca ataatcgccg
    11341 ctggagcgag aactggatga tcggcattgc cttcagttca tggtcatcgt gggtatttgg
    11401 tacggtaata ggggcattct ccggcagcgg cttgctgcaa ggttatcccg ccgttgaagc
    11461 agcattaggt tttatgcttc cggcactctt tatgagtttc ctgctcgcct ctttccagcg
    11521 caaacaatct ctttgcgtta ccgcagcgtt agttggtgcc cttgcaggcg taacgctatt
    11581 ttctattccc gtcgccattc tggcaggcat tgtctgtggc tgcctcactg cgttaatcca
    11641 ggcattctgg caaggagcgc ccgatgagct atgaggttct gctgcttggg ttactggttg
    11701 gcgcggcgaa ttattgcttc cgctatttgc cgctgcgcct gcgtgtgggt aatgcccgcc
    11761 caaccaaacg tggcgcggta ggtattttgc tcgacaccat tggcatcgcc tcgatatgcg
    11821 ctctgctggt tgtctctacc gcaccagaag tgatgcacga tacacgccgt ttcgtgccca
    11881 cgctggtcgg cttcgcggta ctgggtgcca gtttctataa aacacgcagc attatcatcc
    11941 caacactgct tagtgcgctg gcctatgggc tcgcctggaa agtgatggcg attatataac
    12001 ccacaagaat catttttcta aaacaataca tttactttat ttgtcactgt cgttactata
    12061 tcggctgaaa ttaatgaggt catacccaaa tggatagttc gtttacgccc attgaacaaa
    12121 tgctaaaatt tcgcgccagc cgccacgaag attttcctta tcaggagatc cttctgactc
    12181 gtctttgcat gcacatgcaa agcaagctgc tggagaaccg caataaaatg ctgaaggctc
    12241 aggggattaa cgagacgttg tttatggcgt tgattacgct ggagtctcag gaaaaccaca
    12301 gtattcagcc ttctgaatta agttgtgctc ttggatcatc ccgtaccaac gcgacgcgta
    12361 ttgccgatga actggaaaaa cgcggttgga tcgaacgtcg tgaaagcgat aacgatcgcc
    12421 gctgcctgca tctgcaatta acggaaaaag gtcacgagtt tttgcgcgag gttttaccac
    12481 cgcagcataa ctgcctgcat caactctggt ccgcgctcag cacaacagaa aaagatcagc
    12541 tcgagcaaat cacccgcaaa ttgctctccc gtctcgacca gatggaacaa gacggtgtgg
    12601 ttctcgaagc gatgagctaa cgcgtcatct cgctcaaaaa tccagattta taaaagaaaa
    12661 aatgaccggc cagcatcgca acatgctggc ctttttggca agcaggtcgg ctcagccgat
    12721 gagttaagaa gatcgtggag aacaatatga gcgcaaatgc ggagactcaa accccgcagc
    12781 aaccggtaaa gaagagcggc aaacgtaagc gtctgctcct ccttctcacc ttgctcttta
    12841 taattattgc cgtagcgata gggatttatt ggtttttggt actgcgtcat ttcgaagaaa
    12901 ccgatgacgc atacgtggca gggaatcaga ttcaaattat gtctcaggtg tctggcagcg
    12961 tgacgaaagt ctgggccgat aacaccgatt ttgtaaaaga aggcgacgtg ctggtcactc
    13021 tcgacccgac agatgctcgc caggcgtttg aaaaagccaa aactgcactg gcttccagcg
    13081 ttcgccaaac ccaccagctg atgattaaca gcaaacagtt gcaggcgaat attgaggtgc
    13141 agaaaatcgc cctcgcaaaa gcacaaagcg actacaaccg ccgtgtgccg ctgggcaatg
    13201 ccaacctgat tggtcgcgaa gagctgcaac acgcccgcga cgccgtcacc agtgcccagg
    13261 cgcaactgga cgtcgctatt caacaataca atgccaatca ggcgatgatt ctggggacta
    13321 aactggaaga tcagcctgcc gtgcaacagg ctgccaccga agtacgtaac gcctggctgg
    13381 cgctggagcg tacccgtatt gtcagtccga tgaccggtta tgtctcccgc cgcgcggtac
    13441 agcctggggc gcaaattagc ccaacgacgc cgctgatggc ggtcgttcca gccaccaata
    13501 tgtgggtgga tgccaacttt aaagagacgc agattgccaa tatgcgtatc ggtcagccgg
    13561 tcactatcac cacggatatt tacggcgatg atgtgaaata caccggtaaa gtggttggtc
    13621 tggatatggg cacaggtagc gcattctcac tgcttccggc gcaaaatgcg accggtaact
    13681 ggatcaaagt cgttcagcgt ctacctgtgc gtatcgaact ggaccagaaa cagctggagc
    13741 aatacccgct gcgcattggt ttgtccacac tggtgagcgt caataccact aaccgtgacg
    13801 gtcaggtact ggcaaataaa gtacgttcaa caccggtcgc ggtaagtacc gcgcgtgaaa
    13861 tcagcctggc acctgtcaat aaactgatcg acgatatcgt aaaagctaac gctggctaat
    13921 ccagaggtgc gtgtgatgca acagcaaaaa ccgctggaag gcgcgcaact ggtcattatg
    13981 acgattgcgc tgtcactggc gacattcatg caggtgctgg actccaccat tgctaacgtg
    14041 gcgatcccca ctatcgccgg gaatctgggc tcatcgctca gccagggaac gtgggtaatc
    14101 acttctttcg gggtggcgaa tgccatctcg atcccgctta ccggctggct ggcaaagcgc
    14161 gtcggggaag tgaaactgtt cctttggtcc accatcgcct ttgctattgc gtcgtgggcg
    14221 tgtggtgtct ccagcagcct gaatatgctg atcttcttcc gcgtgattca ggggattgtc
    14281 gccgggccgt tgatcccgct ttcgcaaagt ctattgctga ataactaccc gccagccaaa
    14341 cgctcgatcg cgctggcgtt gtggtcgatg acggtgattg tcgcgccaat ttgcggcccg
    14401 atcctcggcg gttatatcag cgataattac cactggggct ggatattctt catcaacgtg
    14461 ccgattggcg tggcggtggt gttgatgaca ctgcaaactc tgcgcggacg tgaaacccgc
    14521 accgaacggc ggcggattga tgccgtgggg ctggcactgc tggttattgg tatcggcagc
    14581 ctgcagatta tgctcgaccg cggtaaagag ctggactggt tttcatcaca ggaaattatc
    14641 atccttaccg tggtggcggt ggtggctatc tgcttcctga ttgtctggga gctgaccgac
    14701 gataacccga tagtcgatct gtcgttgttt aagtcgcgca acttcaccat cggctgcttg
    14761 tgtatcagcc tcgcgtatat gctctacttc ggcgctattg ttctgctgcc gcagttgttg
    14821 caggaggtct acggttacac ggcgacctgg gcaggtttgg cctctgcgcc ggtagggatt
    14881 attccggtga tcctgtcgcc gattatcggc cgcttcgcgc ataaactgga tatgcggcgg
    14941 ctggtaacct tcagctttat tatgtatgcc gtctgcttct actggcgtgc ctataccttt
    15001 gaaccaggta tggattttgg cgcgtcggcc tggccgcagt ttatccaggg gtttgcggtg
    15061 gcctgcttct ttatgccgct gaccaccatt acgctgtctg gtttgccacc ggaacgactg
    15121 gcggcggcat cgagcctctc taactttacg cgaacgctgg cggggtctat cggcacgtcg
    15181 ataaccacga ccatgtggac caaccgcgag tcgatgcacc atgcgcagtt gactgagtcg
    15241 gtaaacccgt tcaacccgaa tgcccaggcg atgtacagtc aactggaagg gcttgggatg
    15301 acgcaacagc aggcatcagg ctggattgcc cagcagatca ccaatcaggg gctgattatt
    15361 tccgccaatg agatcttctg gatgtcagcc gggatattcc tcgtactgct ggggctggtg
    15421 tggtttgcta aaccgccatt tggcgcaggt ggcggtggcg gcggtgcgca ctaagtacaa
    15481 ctaagccagt tcatttgaac tggctttttt caattaattg tgaagatagt ttactgacta
    15541 gatgtgcagt tcctgcaact tctctttcgg cagtgccagt tcttcgttgc tgttgatgcg
    15601 tacgtcacgt tccagaatgc tacgcgcaat atcctgcgct tcctgcaacg agtgcatctg
    15661 gtaagtgcca cactggtaga cgttcagttc cgggatctga ttctgatcct gcactttcag
    15721 cacgtcttcc attgccgctt tccaggcatc agcaacacgc tgctcatctg gcgtaccaat
    15781 cagactcata taaaaaccgg tgcggcagcc cattggcgag atatcgataa tctctacacc
    15841 attaccgtta agatggttac gcataaaacc agcaaacagg tgctccaggg tatggatccc
    15901 tctttctggc atcacttctt tgttcggcac gcagaagcgc agatcgaaca cggtgattgc
    15961 gtcgccatgc ggggtgttca ttgttttcgc cacccgaact gcaggcgctt ccatccgggt
    16021 atgatcgact gtgaagctat ctaacaacgg catttagcca cctccggtaa tttttttaaa
    16081 aattttctga actctttctt cccaggcgag tctgagtata tgaaagacgc gcatttgtta
    16141 tcatcatccc tgaattcaga gatgaaattt tggccactca cgagtggcct ttttcttttc
    16201 tgtcaggtgt gtttttccag ccacaccgca aacggttcgg tatcagcggt ttccatttcc
    16261 tgctgacggc gttcagatgc ctcgcgctcg gctacaaaat cctcttcgcg cagaatttcc
    16321 agcggctctt cacgcagcag attacggtag gcttctgcaa acgctttgcc tgttccgcca
    16381 ataccagtat caatcataga ccttaagata cgggcagaga aagtcagatc gggattatcg
    16441 aagcaggcaa ccagttcatc acacactttc tgatacgcgt cgccgccgtt aatgctatcc
    16501 agcgtttgcg cgacgcgttt cagatcgcgg aacagatctt tacccacctg cagtaacggg
    16561 aactgcgcgg tttcgcagcc gatacccagc gtcaggcccg gtttgcgacc ttcaaggatc
    16621 acccggttcc agttaacgcg cgtacaggca agttcgctac tgctcatttc cggtgcatca
    16681 gccagcgcac accagaccat aaacaggtcg aggaatcgca cctgctgttc atctacacca
    16741 atcggcgaga acgggttgat gtccagcgaa cgcacttcaa tatattcaat gccgccacgt
    16801 aacagcgcat cagaaggtga ctcgccgctg cgggtaacgc gttttggacg aatcggcgca
    16861 tacagttcgt tttcaatctg cagcacgttg ctattgattt gcagcctctt accgtctttc
    16921 tcaataccaa tcttcgcgta ctcttccgat ggcgttttga ttgcctgttt aaggcccgct
    16981 acgtactcgt aaagatcgtt gaaggtaata ccaagattgc tttgcgattt attggtatag
    17041 ccgagatcgc tcaaacgaag agaggtcgca tacggcaggt aatacatacc gcactcggtt
    17101 ttctcaaacg gcagcgacgt tggttttcct tgcaggaaag aagaacaaat cgccggagat
    17161 gcaccaaaca gataaggaat gacccaaccg aaacgatagt aattgcggat aacgcggaaa
    17221 tagcccgcag aaattttctc tttggcatca gcgcccgaga tatcaccgca cttcgcttgc
    17281 cagaatgcca ttggcaaaga gaaattgtag tgcacgccgg aaatggtttg catcagcgcg
    17341 ccgtagcgat ttttcagccc ttcacgatac agcgttttaa agcgtccggt gttagaagtg
    17401 ccgtactgtg ccagttcgat gtcctgacct tctgcgatgt agcatggcat acttaacggc
    17461 cacatccgct catcgcccat attgcgcgcc gtataacgat gcagatcgcg cataaaggtc
    17521 agcatatgtt caatatcacc atccactggt gtaatgaatt ccagcaatgc ttccgcaaaa
    17581 tcggtagtaa tccatttgtg cgtcagtgcg gaacctaatg cttcaggatg acctgttgtt
    17641 gccagtgtgc catcagcatt aacacgcaaa gtttcgcgct ccagcccacg ctgtatcccc
    17701 tttaacgcct gaggatgttt ttccagccag gccagcgcct gtgatacgtc cgggatcaaa
    17761 ttgacctccc gcctgtcaaa atcgttttaa ttagcataac tgtaatggtg accatatgtg
    17821 caggcctaca attagtgcca ccacatcatg ccctgaacgg tcgctgctgc aactgcaaca
    17881 tagcgtaacg ctttaccaag gcacaaaaaa aagattaccg gtccccacga gatgcgcatc
    17941 catcccgcta acagacacag taaatcgcca accacgggca tccagcttaa taatagcgtg
    18001 actgcaccat agcgtttcag ccagccgata gctttctctt gccagcgcga tgttttacgc
    18061 aatggaaaga aacgcccaag gataacgtta gttaaccctc caaggctatt acccattgtt
    18121 gctgttaaaa ctaaaaccca gggatgactg atcccggaaa gcaacattgc caccagcacg
    18181 acttcggagt tgccgggtaa tagtgtagcg ctgagaaaac tactggcaaa caacgagaaa
    18241 agcgataacg cttcactcac agcaagcgaa cgtccacagc gtccatacct gcggcacggg
    18301 ccgcctgaat accgaaatcg gcatcttcaa agaccacaca ctgcgtcggt tgcacgccca
    18361 tacgctgcgc gcacaacaaa aatgtgtctg gcgcgggttt atggtgtttg acgtgatcgg
    18421 cagcgacgac ggcgtcaaaa taatggcgta atcccaggtg cgccagcaat gcctcagcga
    18481 tggcgctttc actccccgtt cctacagcca ttgggcgacg accatgccaa cttttcacca
    18541 catcaacaag aggaagcggt tcgacgctat ccagcagcat acttcttact gcttctgttt
    18601 tttcacgcgc taacgcatgc gggtcgagat cggcctgatt cagctcaata attgcctgag
    18661 caatacgcca ggtgggcgat ccattaagcg caatcatcgc ctgaatatcg tactgaagac
    18721 cgtagtgccc taatacttcg cgccacgctt tacggtgcgt aggctccgta tccaggattg
    18781 tgccatccat atcaaaaatt aaacctgcat aacgctcgta catggtcttc tcgcaagtcg
    18841 aaaaatatga cgctacttta gcgtaattgc ttgattttgt cgctgatggc gaaggagaag
    18901 aaaattcagg aaatggataa aggagttatt tagaaatgag atatttttga aggaaatttt
    18961 ttggagaaga tggtgcatcc gggaggatga ctcactgacg ttcgcccttc gggccgttgc
    19021 tggcgcaacg ttatccttcc tggtgcctgc ggttaatacc gctatctctt cgataccttc
    19081 attgctgaag gttacggaga atatggtgca tccgggagga ttcgaacctc cgaccgctcg
    19141 gttcgtagcc gagtactcta tccagctgag ctacggatgc atcgggaaaa cttattctac
    19201 tgctgatatt gataccgcta ctaaagccat atcaattaag agatggtgca tccgggagga
    19261 ttactcgcct tgcggctcgc ccttcgggcc gttgctaaag caacgttatc atccctggtg
    19321 cctgcggtta attccgctac ctcttcgata ccttcattgc tgaagatttc ggagaatatg
    19381 gtgcatccgg gaggattcga acctccgacc gctcggttcg tagccgagta ctctatccag
    19441 ctgagctacg gatgcatcgg gaaaatttat tttactgctg atattgatac cgctactaaa
    19501 gccatatcaa gtaagagatg gtgcatccgg gaggattcga acctccgacc gctcggttcg
    19561 tagccgagta ctctatccag ctgagctacg gatgcaaatg gcggtgaggc ggggattcga
    19621 accccggatg cagcttttga ccgcatactc ccttagcagg ggagcgcctt cagcctctcg
    19681 gccacctcac cacacgcctc ttacgagtgc ttcgaagaac ttgtttatcg ctcatcgtcg
    19741 ctgcgtggcg cacatattac tttctgagac ttataagtca aacaattttt cccacacttt
    19801 tatcgtttgc acacttcacg ctcaattagt ctgtaaaaac gtcaaaaaga gtgttttatc
    19861 tacagaaaaa cggaggtctg gcagatagca gtaatgcaaa aaaatggaga cttaagttga
    19921 atgaacggga gtaaagcgaa aagactatag agtgaaggag aaattttgag ggtgcgtctc
    19981 accgataaag atgagacgcg gaaagattag taactggact gctgggattt ttcagcctgg
    20041 atacgctggt agatctcttc acggtgaaca gaaacttcct tcggggcatt tacgccaata
    20101 cgtacctggt tgccctttac ccctaaaact gtcacggtga cctcatcccc aatcatgagg
    20161 gtctcaccaa ctcgacgagt cagaatcagc attctttgct ccttgaaaga ttaaaagagt
    20221 cgggtctctc tgtatcccgg cattatccat catataacgc caaaaagtaa gcaatgacaa
    20281 acacattaca tgtaagcagt catggcatta cattctgtta aacctaagtt tagccgatat
    20341 acacaacttc aacctgactt tatcgttgtc gatagcgtta atgcgaatgc cgtgaagcga
    20401 gtccacggca ttgcctgacg cttatattat tgcaatttcg cgctgaccca gcctttcaca
    20461 ctggctaacg ctgcaggtaa ggccgcagca tccgtaccac cggcttgcgc catgtcagga
    20521 cgtccaccac ccttgccgcc cacctgctga gcgaccatac caatcagttc ccctgctttc
    20581 acacgatctg tgacgtcctt agatacgcct gcaatcagag aaaccttacc ttcggctacc
    20641 gttgccagca cgataattgt cgaccccagc tgatttttta aatcgtcaac catggtacgc
    20701 aacattttcg gctcaacacc gctaagctcg ctaaccaaca gcttaacacc attaacatca
    20761 attgccttac tggaaagatt tgcgctctcc tgtgcggcag cttgttcttt aagctgttgt
    20821 agttcttttt ctaactgacg cgtacgttcc agtactgagc gcactttatc cgccagatta
    20881 ttgctatcgc ctttcagcag atgcgcgact tcgcttaagc gatcgctgtc tgcatgaacg
    20941 gtggtgatag cgccttctcc ggttaccgct tcgatacgac gaacgcctgc agcagtaccc
    21001 gattcagaga tgatgcggaa cagaccaata tcaccagtgc ggctggcgtg agtaccgcca
    21061 cacagctcgg tggagaaatc gcccatgctc agcacgcgta cacgctcatc atacttctcg
    21121 ccgaacagcg ccatcgcacc tttcgctttc gccgcttcga gatccatgat gttggtttcg
    21181 atcggcaaat tgcgacgaat ctgtgcgttc accaggtctt cgaccgcacg aatctcttcc
    21241 ggtttcatcg cttcgttgtg tgagaagtcg aagcgcagca ccttgtcgtt aaccagtgaa
    21301 cctttctgcg atacatgagt ccccagaacc tggcgcagcg cagcgtgcat caggtgcgtt
    21361 gcggagtgat tcagacgaat acgggcgcga cgagcctcat caacatcagc ctgcacagcg
    21421 tcgcccactt tcagagaacc cgcagcaagt ttaccgatgt gaccaatcgc ctggccgtat
    21481 ttctgcgtat cttccaccgc gaaggagaag ttagcgcctt tcagttcgcc tttatcacca
    21541 acctgaccgc cggattccgc atagaatggc gtttgatcca gcacgaccac agcttcctgg
    21601 cctgcattga tggcatcaac cgctttacca tcaacaaaca gcgcggtcac tttgccgttc
    21661 agttccagat ggtcatagcc tttaaattca gatgcactat caacacggat cattgcgttg
    21721 taatcggcac caaagccgct ggcttcgcgc gcgcgacgac gctgctcttc cattgctgct
    21781 tcaaaaccag cttcgtcaac tttgatgttg cgctcacgac aaacatcagc cgtcaggtca
    21841 accgggaagc cataggtgtc gtacagacgg aaagccgttt caccatccag cgtatcacca
    21901 gaaagttttg ccagctcttc atccagcaac gccagaccgc gctccagagt acgggcaaac
    21961 tgctcttctt cagtcttcag cacctgctca acctgcgcct gctggcgttt caggtcttca
    22021 cccgcagagc ccataacgtc gatcagcgga ccaaccagtt tgtagaagaa ggtttctttc
    22081 gcgccgagca tattgccgtg acgcactgcg cgacgaatga tacgacgcag tacataacca
    22141 cggttttcat tggacggcat tacgccatcc gcgatcagga acgcacaaga acgaatgtgg
    22201 tcagcgatta cgcgcagcga tttattgctc agttcggtcg cgccagtgac tttcgctacc
    22261 gcctggatca acgtgcggaa caggtcgatg tcatagttag agttaacgtg ttgcagcacc
    22321 gcagcaatac gctccagacc cataccggta tctacagacg gcttcggcag cggttccatc
    22381 gtgccatctg cctggcggtt gaactgcatg aagacgatgt tccagatctc aatgtagcgg
    22441 tcgccgtctt cttccggact tcccggaggg ccaccccaaa tgtggtcgcc gtgatcgtag
    22501 aagatttcgg tgcacgggcc gcacggacca gtgtcaccca tctgccagaa gttgtcagat
    22561 gcgtatggcg cacctttgtt atcgccgatg cgaataatac gttcgcgcgg aatccctact
    22621 tctttttccc agatttcgta ggcttcgtcg tcgctttcat agacggtaac ccacagacgc
    22681 tctttcggca gggcaaacca tttttcgctg gtcagcagtt cccatgcaaa ctgaatggca
    22741 tcatgtttga aatagtcgcc gaagctgaag ttgcccagca tttcgaagaa ggtatggtga
    22801 cgcgcggtgt aaccgacgtt ttccaggtcg ttgtgtttac cacccgcacg cacgcagcgt
    22861 tgggaagtgg tagcgcggga ataattacgc ttgtcgagcc caaggaacac atccttgaac
    22921 tggttcatcc cggcgttggt aaacaacaaa gttgggtcgt tatggggtac cagggagctg
    22981 ctggcaacta cctgatgtcc cttactatgg aaaaagtcga gaaacgcctg acggatctca
    23041 gcggtgctct tgctcataat tatcctgaaa tcaagctaac gaaatatcgc caccagctcc
    23101 agcgtgtctt aaccgccggg ctggtaactg aaaagtggga ataagataag ttttcttgag
    23161 tgggaagtaa aataccgtat gcgttcagtc ggcaaaattt cgccaaatat cctggatatc
    23221 ttccatcaga tagccacgat agagcagaaa acgctggatc ttaacttttt ctgaaaagac
    23281 agttggcaaa ggttcaccat attttcgcgt cgcctgatcg cgcaccagtg cgcaccagtc
    23341 gatgtcacat tcacgcatcg ctttttctgt cgcttcgcgg gaaatacctt tctgattcag
    23401 ttcctgacga atacgtgcag gtccataacc tttgcggcta cggctggcga taaagcgcgc
    23461 aacaaatcgg ctgtcatcga gatagccatg ttcatggcac caggcaataa cgcgctcgta
    23521 atcttctgcc gtagcatcaa tctcttctgg gccatttttg cccataatcg gtgccgcgag
    23581 tttacgtcgc agttcttgct cactgtgatc gcgcaccgcc agaatgcgta ccgcacgatc
    23641 caacaggcga gcatatgccg ggcgacggga tgttgattct gtcatggcat atccttacaa
    23701 cttaaaaaag caaaagggcc gcagatgcga cccaggtttt gctgataaac agatttaata
    23761 gtcaatgagt gtgcagccaa ttcgttagag gatcgaggtg agagtaattt ttcacgccag
    23821 tgttggcctg ttcccctcac cctaaccctc tccccaaagg ggcgagggga ccgtccgtgc
    23881 acacattatc tattatcatc aataacgaac gattaaaaat cttcgttagt ttctgctacg
    23941 ccttcgctgt catctacaga gaaatccggc gttgagttcg ggttgctcag cagcaactca
    24001 cgtactttct tctcgatctc tttcgcggtt tccgggttat ctttcagcca ggcagttgca
    24061 ttcgctttac cctgaccgat cttctcacct ttgtagctgt accacgcgcc tgctttctcg
    24121 atcagcttct cttttacgcc caggtcaacc agttcgccgt agaagttgat accttcgccg
    24181 tagaggatct gaaattcagc ctgtttaaac ggtgcagcga ttttgttctt caccactttc
    24241 acgcgggttt cgctacccac cacgttttcg ccctctttca ccgcgccgat acgacggatg
    24301 tcgagacgaa cagaggcgta gaatttcagc gcgttaccac cggtagtggt ttccgggtta
    24361 ccgaacatca caccaatttt catacggatc tggttgatga agatcagcag cgtgttggac
    24421 tgcttcaggt tacccgccag cttacgcatc gcctggctca tcatacgtgc cgcaaggccc
    24481 atgtgagagt cgccgatttc gccttcgatt tccgctttcg gcgtcagtgc cgccacggag
    24541 tcaacgacga taacgtctac tgcgccagaa cgcgccaggg cgtcacagat ttccagtgcc
    24601 tgctcgccgg tgtccggctg ggagcacagc aggttatcga tatcgacgcc cagtttacgt
    24661 gcgtagattg ggtccagcgc gtgttcagca tcgataaacg cacaggtttt accttcacgc
    24721 tgcgctgcgg cgatcacctg cagcgtcagc gtggttttac cggaagattc cggtccgtag
    24781 atttcgacga tacggcccat cggcagacca cctgccccaa gcgcgatatc cagtgaaagc
    24841 gaaccggtag agatggtttc cacatccatg gaacggtctt cacccaggcg catgatggag
    24901 cctttaccaa attgtttctc aatctggccc agtgctgccg ccaacgcttt ctgtttgttt
    24961 tcgtcgatag ccatttttac tcctgtcatg ccgggtaata ccggatagtc aatatgttct
    25021 gttgaagcaa ttatactgta tactcataca gtatcaagtg ttttgtagaa attgttgcca
    25081 caaggtctgc aatgcatacg cagtagcctg acgacgcacc gcatcacggt cgccgctgaa
    25141 gcattcccgc cgggtaatgc cttcaccgcg ggcagtggca aaagcaaacc agacggtgcc
    25201 gacaggcttc tcttcactgc cgccatccgg cccggcgata ccactaatag acacggcata
    25261 atcagcacga gccgctttca gtgcacctat cgccatttcc accacgacgg gttcactcac
    25321 cgcgccacgc tgcgccagcg tctcttcgcg tacgccgatc atctgcgctt tggcttcgtt
    25381 actgtaggtg acaaatccgc gttcaaacca ggcggagcta ccggcaatat cggtaatcac
    25441 tttcgctacc caaccaccgg tacaagactc ggcagttgtt acggttgcgc cacgggcttt
    25501 cagcgcctgc ccaacctgtt cgcttaactg catcagttca ctgtcagtca tcacaacctc
    25561 agtcagtcaa aaatttatgc cggacaagat agcactttcg tcaggaagat ggtgttggcg
    25621 ttgcaatttt acgaggagga ttcagaaaaa agctgattag ccagagggaa gctcacgccc
    25681 ccctcttgca aatagttact gcacgcgcgc cagcgccacg gcttgtccta actgccagac
    25741 cgccattgcg taatgggtgc tgtggttata acgggtaatg gtgtagaagt tcggcagacc
    25801 gtaccagtac tggtaaccgg taccgacatc cagacgcagc aggctggctt gttgatggtt
    25861 gcccagcggc tgctgtggcg ttaaacctgc cgtggcaagc tgcgaaatgc tgtacctggt
    25921 tttgaagcca tttggcaagc ctggagcctg accgtttgcc attaccgcga cctgatcgcc
    25981 tttcacccag ccgtgcgctt tgaaatagtt cgccacacta ccgatcgcat caaccggatc
    26041 ccacaggttg atatgcccgt cgccgctgaa atctaccgca tattgtttgt aagacgacgg
    26101 cataaactgt ccgtagccca tcgccccggc aaaggaacct ttcagattga gcggatcgtc
    26161 ctgctcgtcg cgcgccatca acaggaaggt ttccagctcg ccagagaaat actccgcgcg
    26221 gcgtgggtag ttaaatgaca gcgttgccag cgcatcgagg atgcgagttt tccccatcac
    26281 gcgcccccag cgggtttcaa cgccgataat cccgacgata atttccggcg gtacgccata
    26341 cacctgccac gcgcgattca acgcatcttc atactgattc cagaaaacca caccgttctg
    26401 cacgttgtcc ggcgtaataa attttttgcg ataacgcagc catgcgccgt tcggacctga
    26461 tggcggtttc accgatgtgg ttggtgcctg gttatccatc agccgcagta ccgaatccag
    26521 acgcttcgcc tgggagagaa tttcctgcaa ctgctgacga tcgaaaccgt gtttgttcac
    26581 cattctgtcg atgaactgct gggcattcgg gttattagcg aaatcgccgc ccatctgcat
    26641 cacattgtgc tgaggctcaa gcaggaagcc gccagacggc gttccggtgg tcgtttcagt
    26701 ctcagtaggt tttggcttgc tgctacaggc ggcaagcaac acaaaaaggg gaagcaatgt
    26761 tacataacga cgcttgaaca tgaggggtcc atttaacaga ttcaaccagg ggcaagtatg
    26821 gtaaagcatc acgccccgca caaggaagcg gtagtcactg cccgatacgg actttacata
    26881 actcaactca ttcccctcgc tatcctttta ttcaaacttt caaattaaaa tatttatctt
    26941 tcattttgcg atcaaaataa cacttttaaa tctttcaatc tgattagatt aggttgccgt
    27001 ttggtaataa aacaataaat cctgaaggag agaacaatga tagaaaccat tactcatggt
    27061 gcagagtggt ttatcgggct gttccaaaag ggcggagagg tgtttaccgg gatggtgacc
    27121 ggcattcttc cgctgttgat tagcctgctg gttatcatga acgcactgat taattttatc
    27181 ggtcagcatc gtattgaacg ttttgctcaa cgttgcgccg gtaaccctgt ttcccgttac
    27241 ctgctgctac cgtgcattgg cacgtttgtc ttttgcaatc cgatgacctt aagcctgggt
    27301 cgctttatgc cggaaaagta caaacccagc tactacgcgg cggcctctta tagctgccac
    27361 tcaatgaatg gcctctttcc ccatatcaac cctggcgaac tgtttgttta tcttggcatt
    27421 gccagcggtc tgacaacgct gaacctgcca cttggcccac tggcggtgag ctatctgctg
    27481 gttggtctgg tcaccaactt ctttcgcggc tgggtgaccg atctgaccac cgccattttt
    27541 gagaaaaaga tgggcattca acttgaacaa aaagttcacc tggcaggagc aacatcatga
    27601 cgcgtattcg gatcgaaaaa ggaacgggtg gctggggcgg cccgcttgag ctggaagcca
    27661 cgccgggcaa aaaaatcgtc tatatcaccg ccggtacccg gcctgcgatt gttgacaaac
    27721 tggcacaact tactggctgg caggctattg acggatttaa agaaggtgaa cccgcggagg
    27781 cggaaattgg tgtcgcggta atcgactgtg gcggcacatt acgctgcggc atctatccga
    27841 aacgacgtat tcccaccatt aatatccact cgacgggcaa gtccagtccg ctggcgcagt
    27901 acattgtgga agatatttat gtctctggcg taaaagaaga aaacatcact gtagtgggtg
    27961 atgcgacacc acaaccctct tccgtgggcc gtgactatga caccagcaag aaaatcaccg
    28021 aacaaagcga tggtttactg gcgaaggtgg gaatgggtat gggttctgcc gttgccgtgt
    28081 tgtttcaatc tggtcgtgac accatcgaca ctgtattaaa aaccattctg ccgtttatgg
    28141 cgttcgtctc ggcgctcatt ggcatcatta tggcttctgg ccttggtgac tggattgccc
    28201 acggtcttgc tccgctggcg agccatccac tgggtctggt catgctggcg ctcatctgct
    28261 ccttcccgct gctttcacct ttcctcggcc caggcgcagt tatcgcacag gttatcggcg
    28321 tattgattgg cgtgcagatt ggtctcggca atattccgcc gcatctggct ttaccggcac
    28381 tgtttgccat caacgcgcag gcggcctgcg acttcatccc ggtcggtttg tcgctggcgg
    28441 aagcccgtca ggacacggtt cgcgtcggtg tcccttctgt actggtgagc cactttttaa
    28501 ccggcgcacc gactgtactg atcgcctggt ttgtctccgg ttttatctat caatagaggc
    28561 tgaaacatga ccgttattta tcagaccacc atcacccgta tcggcgcgag tgccactgac
    28621 gccctcagcg accagatgct catcaccttt cgtgaaggcg cgccagcgga cctcgaagag
    28681 tattgcttca ttcattgcca cggcgagttg aaaggtgcac tccatcccgg tttgcaattt
    28741 acgctcgggc agcatcgcta tccggtgacc gctgttggca gcgtggcgga agacaacctt
    28801 cgcgaactgg gtcatgtcac cctgcgcttc gatggtttaa acgaagcgga atttccgggc
    28861 actgtccatg tggcaggccc tgtacccgac gatatcgcgc cgggatcggt tttgaagttt
    28921 gaatctgtta aggagtaaaa aatgaatcag gttgccgttg tcatcggtgg tgggcaaacc
    28981 ttaggcgcgt tcctgtgcca cggtctggct gccgaggggt atcgcgtcgc ggttgtcgat
    29041 attcagagcg acaaagccgc aaatgtggca caagaaatta acgccgaata tggtgaagga
    29101 acagcgtata gttttggtgc tgacgccacc agcgagcaaa gcgtcctggc gctctctcgt
    29161 ggggtagatg aaatctttgg tcgcgtggat ttgctggtct acagcgccgg aatagcaaaa
    29221 gcagccttta tcagcgactt ccagctcggc gattttgacc gtgaatctgg tgggttattt
    29281 cctgtgtgcc cgtgaatttt ctcgtttgat gatccgcgac gggattcagg gtcgcattat
    29341 tcagatcaac tcgaaatccg gcaaagtggg cagcaaacac aactctggct acagcgcagc
    29401 gaaatttggt ggcgtcgggc tgactcaatc actggcgctg gatctggcgg agtacggcat
    29461 tacggtgcat tcactgatgc tcggtaacct gctgaaatcg cctatgttcc agtcactgtt
    29521 accccaatac gcgaccaagc tgggtatcaa accggatcaa gtcgagcagt attacatcga
    29581 caaagtaccg ctcaaacgcg gctgcgatta tcaggatgtg ctgaatatgc tgctgctcta
    29641 tgccagtcct aaggcgtcgt actgcaccgg acagtcgatc aatgtcaccg gcggtcaggt
    29701 gatgttctaa tcaacagcgg agatccgtta aggatctccg tgagactaat agaatgcctg
    29761 atgcgctacg ctcatcaggc atacaggact tccgccacta cattaaggaa aagttatggt
    29821 atccgcactc atcaccgtcg ccgttatcgc ctggtgtgcg caactggcct taggcggctg
    29881 gcaaatttct cgttttaacc gtgccttcga cacactatgc cagcaagggc gggttggcgt
    29941 gggccgttcc agcgggcgct ttaaaccacg ggtcgtggtc gccatcgccc tggacgatca
    30001 gcagcgcatc gtcgacacct tgtttatgaa aggactgacc gtcttcgcac gaccgcaaaa
    30061 aattcccgca attaccggta tgcatgcggg tgatttacag cccgatgtga tctttcccca
    30121 tgatccacta tcacagaatg ctctatcatt ggcgcttaaa ctgaaacgtg gataatttcg
    30181 ttgtgaatgt tacttgcttg cgaagttatc attttgaaac ctaaatcagg taatcacgcc
    30241 catgaaacct cgtcagcgtc aggccgccat tctggagtat ctgcaaaagc agggtaaatg
    30301 ctcggttgaa gaattggcgc aatactttga caccacaggc acaaccattc gcaaagatct
    30361 ggtcattctg gaacatgcag gaaccgtcat tcgtacttat ggcggagtgg tgttgaataa
    30421 agaggaatcc gatccgccta tcgatcataa aacactcatc aacacccaca agaaagagct
    30481 gatagcagaa gctgccgtta gttttattca tgatggcgat tcgatcattc ttgatgctgg
    30541 cagtaccgtt ttgcagatgg ttcccctgct ctcgcgcttt aataacatca cggtgatgac
    30601 caacagtctg catatcgtca atgcgctatc tgaactggat aacgaacaaa ctatcctgat
    30661 gccaggcgga acgtttcgta aaaaatcggc ctcatttcac ggacagctgg cagagaatgc
    30721 cttcgaacat ttcagtttcg ataaattgtt tatgggcacc gacggcatcg atctcaatgc
    30781 gggcgtaacc acctttaacg aggtttatac cgtcagtaag gcaatgtgca atgccgcgcg
    30841 cgaagtgatt ttgatggcgg actcatcaaa gtttggccgt aaaagcccca acgtagtttg
    30901 cagtcttgaa agcgtcgata agctgattac cgacgcaggt atcgatccgg cgtttcgtca
    30961 ggcgctggaa gagaaaggga tcgatgtgat cataaccgga gagagcaatg agtgaagcac
    31021 tactgaacgc gggacgtcag acgttaatgc tggagttgca ggaagcaagc cgtttaccgg
    31081 aacgtctggg cgatgatttt gttcgcgccg ccaatatcat cctgcactgc gaaggcaaag
    31141 tggtggtttc gggaattggc aaatcgggcc acattggtaa gaaaatcgcc gcaacacttg
    31201 ccagtaccgg cacatcggct ttttttgtcc atccggcaga agcactgcac ggcgatctgg
    31261 ggatgattga aagccgcgat gtgatgctgt ttatctctta ctccggtggc gcgaaggaac
    31321 tggatctgat tattccgcgt ctggaagata aatctatcgc gctgctggcg atgaccggca
    31381 aaccgacgtc accgctgggc ctggcggcaa aagcggtgct ggatatctcc gtagaacgcg
    31441 aagcctgccc gatgcacctt gcgccgacct ccagcaccgt caataccctg atgatgggtg
    31501 acgcgctggc gatggcggtc atgcaggcgc gcggatttaa tgaagaagat tttgcccgct
    31561 cccacccagc cggggcactg ggcgctcgct tgctgaataa agtgcatcat ctgatgcgcc
    31621 gtgacgatgc catcccacag gtggcgttaa ccgccagcgt gatggatgcg atgctggaac
    31681 tcagccgcac cggtctgggg ctggtggcgg tatgtgacgc tcaacaacag gtacaaggcg
    31741 tctttaccga cggcgattta cgtcgctggc tggtaggcgg cggcgcactc accacgccag
    31801 ttaatgaagc gatgacgaca ggcggcacca cgttacaggc gcaaagtcgc gccatcgacg
    31861 ccaaagagat cctgatgaag cgcaaaatca ctgccgcacc ggtggtggat gaaaacggca
    31921 aactcaccgg cgcaattaac ctgcaggatt tctatcaggc cgggattatt taatccttca
    31981 ttcccagacg tttcgccagc cgatgcaggt tggcgacgtc ggtttccagc atccgcgcgc
    32041 aggcagccca gttatgatga ttttgtgcca gtgcctggcg aatagtttca cgctggaacg
    32101 cttctgtcgc ttcacgcagg ttttgcttaa caacgggcac cgccgccgct tctggcggcg
    32161 gcaacgtcac ctcaggaaaa gcaaaatgtt gcgcctcaag aatcacttca tcgccgttgc
    32221 gggtggctct cgccagcact accgcccgat gaatagcatg ttccagttcg cgcacgttcc
    32281 ccggaaaacg atagtgttgc agtaaatttc gcgctccggc acttaatacc acgcgggaga
    32341 gccccagccg caaacgacac tgctcgcaga aataccccgc cagcagaatg acatcatcgc
    32401 cccgctcacg cagcggcggc accgaaagtg gaaacacgct caggcgatga aacaagtcag
    32461 cgcgaaatcg ccctgccatc acctcttcgc gtaagtcgcg gttagtcgcc gccagcacgc
    32521 gcacatcgac ccgcaaacta cggtcatcgc caacgcgctg aatatcgcca tactgcaaca
    32581 ccctcagcag cttggcctgc aatgccaacg acaactcgcc gatctcatcc agaaacagag
    32641 tgccattatc cgccatttca aacttcccgc tgcggttact gatagcgcca gtaaacgctc
    32701 ctttcacatg cccgaacaac tcactttccg ccacactttc cggcagtgca gcacagttga
    32761 gatagaccag cggattcacc gcccgtggcg aggcttcatg aatcgctttc gccaccagct
    32821 ccttaccggt tcccgtctca ccgctgatca ggacgttgag atcggacgcc gccacaatct
    32881 caatctcttt tttcagttgc gtcatgccag gggacaagcc aatcatctgc gtctgtttca
    32941 ccgcttcaaa cggcgtggca tcgcctggca tcatattctg gctttccagt tgttcaatca
    33001 gcaacgcatt gcttaacgct cccgccgcca gcgcagcaat cagccgtagc tcttcgtcgc
    33061 tgaaaacatc gaactgatcg ggctgcatcc cgtcgagcgt cagtgcgccg atcaggtttt
    33121 gcccggcaaa caatggcaga ccaacgcagg cgtgaacctt cagactctcc tgcccaggaa
    33181 tcaaaccgtc atagggatcg ggcaattcgc tgtctgcggg aaagcgcacc acatccccgg
    33241 cgcgggcaat cgcttccagc cgtggatgcc cttccagcgc aaagcgtcta ccgagtacat
    33301 cctttgccag accgtcgatg gcaagcggaa taaactgccg cgaatcgtaa cgtagcaacg
    33361 cagacgcatc gcactccagc acctgacgta gcgtggtgat caggcgctga aaacgatcct
    33421 gatgaccaat cccacgctgc aattcgatgg cgatattcgc cagcacatca acggaaaaac
    33481 tcatctttgc ctcactgtca atttgactat agatattgtc atatcgacca tttgattgat
    33541 agtcattttg actactcatt aatgagcata attttattta tagagtaaaa acaatcagat
    33601 aaaaaactgg cacgtaatct gcaattagca agacatcttt ttagaacacg ctgaataaat
    33661 tgaggttgct atgtctattg tggtgaaaaa taacattcat tgggttggtc aacgtgactg
    33721 ggaagtgcgt gattttcacg gtacggaata taaaacgcta cgcggcagca gttacaacag
    33781 ctacctcatc cgcgaagaaa aaaacgtgct gatcgacacc gtcgaccata aattcagccg
    33841 cgaatttgtg cagaacctgc gtaatgaaat cgatctggcg gatatcgatt acatcgtgat
    33901 taaccatgca gaagaggacc acgctggggc gctgaccgaa ctgatggcgc aaattcccga
    33961 tacgccgatt tactgtactg ccaacgctat cgactcgata aatggtcatc atcatcatcc
    34021 ggagtggaat tttaatgtgg tgaaaactgg tgacacgctg gatatcggca acggcaaaca
    34081 gctcattttt gtcgaaacgc caatgctgca ctggccggac agcatgatga cttacctgac
    34141 aggcgacgcg gtgctgttca gtaacgatgc tttcggtcag cactactgcg acgagcatct
    34201 gttcaacgat gaagtggatc agacggagct tttcgagcag tgccagcgtt actacgccaa
    34261 tatcctgacg ccgttcagcc gcctggtaac accgaaaatt accgagatcc tgggctttaa
    34321 cttaccagtc gatatgatag cgacctctca cggcgtggta tggcgcgata acccgacgca
    34381 aattgtcgag ctgtacctga aatgggcggc tgattatcag gaagacagaa tcaccatttt
    34441 ctacgacacc atgtcgaata acacccgcat gatggctgac gctatcgccc aggggattgc
    34501 ggaaaccgac ccacgcgtgg cggtgaaaat tttcaacgtc gcccgaagcg ataaaaacga
    34561 aatcctgacc aatgtcttcc gctcaaaagg cgtgctggtc ggcacttcga cgatgaataa
    34621 cgtgatgatg ccgaaaatcg ccggtctggt ggaggagatg accggattac gcttccgtaa
    34681 caaacgcgcc agtgctttcg gctctcacgg ctggagcggc ggtgcggtgg atcgtctttc
    34741 cacgcgcctg caggatgcgg gtttcgaaat gtcgcttagc ctgaaagcca aatggcgacc
    34801 agaccaggac gctctggagt tatgccgtga acacggtcgc gaaatcgccc gtcagtgggc
    34861 gctcgcgccg ctgccgcaga gcacggtgaa tacggtggtt aaagaagaaa cctctgccac
    34921 cacgaagtcc gacctcggcc cacggatgca gtgcagcgtc tgccagtgga tttacgatcc
    34981 ggcaaaaggc gagccaatgc aggacgttgc gccaggaacg ccgtggagtg aagttccgga
    35041 taacttcctc tgcccggaat gctctctcgg caaagacgtc tttgacgaac tggcatcgga
    35101 ggcaaaatga gcaacggcat tgtgatcatt ggttcgggct tcgccgcccg ccaactggtg
    35161 aaaaatattc gcaaacagga cgccagtatt ccattaaccc tgatcgccgc cgacagcatg
    35221 gatgagtaca acaaaccgga cctcagccat gttatcagcc aggggcaacg tgccgacgac
    35281 cttacccgcc agacggcggg tgaatttgcc gggcagttta atctgcacct gtttccgcac
    35341 acctgggtga cggatatcga tgccgaagcc catgtggtga aaagccagaa taatcagtgg
    35401 caatacgaca agctggtgct ggcaactggt gccagtgcct ttgtcccgcc agtgcctggg
    35461 cgtgagttaa tgctgacgtt aaatagtcag caagagtatc gcgcctgtga aacgcaactg
    35521 cgggatgccc gacgcgtgtt gattgtcggc ggtggtttga ttggtagcga actggcgatg
    35581 gatttttgtc gtgcaggcaa agcggtcacg ctaatcgaca acgctgccag tattctggcg
    35641 tcattaatgc caccagaagt aagcagccgc ttgcagcatc ggttgacgga gatgggcgtt
    35701 catctgctgt tgaaatctca gttgcagggg ctggaaaaaa cggattctgg cattctggca
    35761 acgctggacc accagcgtag tatcgaagtg gatgcggtaa ttgccgccac cggactgcgc
    35821 ccggaaacag ccctggcacg acgcgccggg ctgacgatta atcgcggcgt ttgcgtcgat
    35881 agttatctgc aaaccagtaa tgacgatatt tatgcgctgg gcgattgcgc ggaaatcaac
    35941 ggtcaggtac tgccattcct gcagccgatt caacttagcg cgatggtgct ggcaaaaaat
    36001 cttctcggca ataacacgcc gctgaaacta ccggcgatgc tggtgaaaat caaaacgccg
    36061 gaattaccgc tgcatctggc aggcgaaacc cagcgtcagg atttacgctg gcaaattaat
    36121 accgaacgcc agggaatggt ggctcgcggt gttgacgatg ctgaccagct tcgcgccttt
    36181 gtggtcagtg aggatcggat gaaagaggca tttggattgt tgaaaacatt gccgatgtag
    36241 gtgggctact gtgcctaaaa tgtcggatgc gacgctggcg cgtcttatcc gacctacggg
    36301 gacgcatgtg taggccggat aaggcgttta cgccgcatcc ggcaatggtg tccaaatgca
    36361 acacgtttta tccgttctgg acttcacccg ctaaccaacg cgccgcagca ataaccccct
    36421 gccccagaga caaaccgcca tcacccgccg gtaaactctg tggaaagagc aatgtgaaat
    36481 cagcgagata atgcgccaga cgtgcacgca gcaaacggtt atgaataacc ccgccgctaa
    36541 ataccagcgt agtgatacca cgcatcgtgg cctgctcacg catcaacgcg gcaaaaccct
    36601 gcgccagcgc atcatgaaac gcccacgcgc gttgattaac cggtgcctgc cagttcagcc
    36661 actgctgcca gaaagcggcg agatccagtt gattgtccac cagcggcatc gtcaccggat
    36721 gcgtcactcc gtggcatgag gctgcgagcg cctccagagc acaagccgct tcaccttcat
    36781 aacttaacgt ggctggcgca cagcccagtg ccgccgccac tgcatcgaac aaccgcccac
    36841 acgatgacgc cagcggcgcg ttaattccac gctcaatggc ccgcgccagc acgctccagt
    36901 tttgctgttg cacacttgct gtttcagggt aattctgcca ctccggcaca aagcgcaggc
    36961 actgcgccag caggtttcgc cacggctgct tcgctgccaa atcgccaccc ggaagcgcca
    37021 ctgcaggcaa gccgcccagg tgctcacatt cgcgatagtt cacccgcagg cactcgccgc
    37081 cccacaaagc gccgttctcc cccataccga taccgtcgag cgtcaaagca atgacatcac
    37141 cgccatccaa cggccactga tgctctgcca gacacgccgc tgcatgggca tgatgatgca
    37201 gtaccgtttg cgtcggcaga ttcatttcac gcgcccactg gctggagaca tagcccggat
    37261 gcgcgtcatg cacaacgtat tgcggggtaa aatcgtagat gttttgcatc aggcgtaacg
    37321 cttcgcgcca ctgcatctgg atgccatcgt cacttaaatc gcccagatgc tgactcaaca
    37381 ccgcttgttc accgcgcacc aggcagaagg tatttttcag atccgcgccg agacacagca
    37441 caggcggaac atttttaaag cccggaggca aagccagcgc atccggcaca tacccccgcg
    37501 aacggcgcag catttcgccg ctttcgcgca ccaccgaatc atccatccgc tgcacgatgt
    37561 cgcggttatg tatcaagaat ccgtcggcaa tgccctgcaa atccgccagc gcctgttcgt
    37621 tgctgatagc tggtggttta ccgctcaggt tgccggaggt catcaccagc gggcattgca
    37681 gttcctgtaa cagcaaatgc tggagcgggt tcgcaggcaa cattaccccg acttcgttaa
    37741 ggtcaggggc gatatcatca caaagctcag gaacgtattt tttatccacc agcacaatcg
    37801 gcgcggcggg cgtggtaagc aactggcgcg cagcgtctgg taaaccgtca gccactggca
    37861 acatgaccgc cagcggtttc gccgggcgat gtttgcgcgc ccgaagtgtc gccaccgcgt
    37921 tactgttacg tgcatcgcag gcaagatgaa atccgccaat ccctttgatg gcgacaatgt
    37981 tgcccatttt taactgtgcg atagccgcct gtaatgccgc ctcttgttct gcatgttcac
    38041 catgacttac ccattcaaga tgtgggccac actccgggca ggccaccggc tgggcgtgga
    38101 agcgacgatc gagcgggtca cggtactctt tgtcacaagc cggacacaac ggaaacgccg
    38161 ccatcacggt aaacgggcgg tcgtaaggca tggcgcgaat aatggtgaaa cgcgggccgc
    38221 agtgggtaca gttgataaac ggataacgat aacgccgttc gcctggggta ttcatttcgg
    38281 caaggcaagc agggcaagta gcggcatccg ggacaatttg cgtattcatg gcacctcccg
    38341 cgctctggcg gatggtgaac tcagtgggca gttgtgacca gatgtacggc tcacgctcga
    38401 cgctatcaat acgcgccagc ggcgggcagt gctgatgcaa ttgaacaagt aacgtttccg
    38461 ggtcttcccg cagccggact tctacgccat cgccgtcatt acagacatcg ccgtgaagat
    38521 ttaattgctg tgccagctgc cagacaaacg gacgaaaacc gacgccctgc actttgccac
    38581 gaatacgcag ttggacaccg caagatgtgt tttttgccat tgagttattc ccgccatcat
    38641 gaattgcgta acccgtcctg ccggacacga ctgcgtcgta tccggcagtc tcaggtcggc
    38701 gataccgcca gctccgtatt ctacgaatat ttccgggaat tcctttgatg ccagaacagt
    38761 tctgtaagat ttttagaaca tcagcgccgt acggcggcgt ttttctgcac tcagttgttc
    38821 aagtttatta cgatcgacac aaatcagcgc atgagtcggg caagccgcca tacacgccgg
    38881 gccgtcttca cgatggttgc acaggtcaca tttattggct tcggctttgt cagcccgtac
    38941 attcagcccc gcgccgctgt tgcgaatcac cggacgtacc accacttcca tcgcgccata
    39001 cgggcaagcc acaacgcagg ttttgcaacc aatgcaacgt tcctgcatca catgaacaaa
    39061 ccctttatca cggctgatag caccattcgg gcagacgtta gcgcacggtg catcttcaca
    39121 ctgacggcaa actgtcgccg tggaaatgtt cacaccttta atgacatgga tacgcggtaa
    39181 aaatgtttcc ggggtcagcg atgcacagtc ctgattttcc tgatgagaaa ccacgcacgc
    39241 cacttcacag gtacggcaac caatacattt actcgcgtca gcaatgatga aacggttcat
    39301 caaattctcc agcaatgaca gttaatgcgc cgatacattc acaaatcatg ccagttttaa
    39361 ttcactgtta tttaaggaaa ttaatttctg taatgcagga aaaaagatgt catcgacact
    39421 agtgacgatg acatgtgatg acaatgttta tcgcgaaaga gcaatgagtg agtcgcggcg
    39481 gatcagtttt ccgctgaagg ttttcggcgg tgagaaatcc ccgccatcga gcataaaaat
    39541 cagccgtcca ataatttcct gaatcatctc agtcaccgga atttttacgc tggagagcgc
    39601 cggaacgata tagggggcaa ttgcgatatc atcgaatccg ataactgaca cctgctctgg
    39661 caccgctacg ccgcgctcgt gcaacgcttt catcgcacct atcgccatat cgtcgttact
    39721 ggcaactaac gcgctaaatt tagccccacg ttcgagcaac gtttctaccc cttcggcccc
    39781 gctggcgggc gtccatttac cgttagcgat aagtttttca ttgagcgcaa taccatgctg
    39841 cgccagcgcg tctttatacc cagcaagacg ttcaatgctg gtgggggaat ccatcgagcc
    39901 ggtaaggaaa gcaatctcct gatgtccggc gtttatcaac tctgccacgg cattaaagct
    39961 ggtctgttta tgatcgcacc agacgctatg gctgctgttt ttgcgcaggc ggcgattaag
    40021 caccattatc ggctgactgt gcgcgtcaat gatgtcatcg atctcatcca cgcttaaaaa
    40081 acgcgggtaa atcataatgg cgtcgcagcg caaatccagc agatactgaa tcgcctggcg
    40141 ctcttcttcg gcgctgtgtt taccatctgc caatagcaac tgccgccctt tctcttccgc
    40201 cattcgcgcg gcatgaaaga gtaattcact aaaataaatg ccgtggtaaa gcgtgttggt
    40261 cactaccaac cccagcgtct gagtactctt cgccgacaga ttgcgcgcca gcaagtttgg
    40321 gcggtaaccg ctctcttcta ccgcctgaaa cacgcgatct ttagtctcct ggctgacgta
    40381 gccattacct gaaagcacgc gggaaacggt cgcttttgaa accccggcgc gcttcgccac
    40441 ttccagcatc gtcgtcatca ttttcatccc tttacacgca atcaacgcag tgtactgcac
    40501 cgtttgccga ttgtccttgc gcaatcggcg ggaaaaatat tcaggtgacc ggtttcacaa
    40561 atataaaaaa tgaacaatta actctcttgc ttatttagtg acaactattc atgattttgt
    40621 gaaaccggtt tcctaattcc gtttcagcat cggcattttt ccgttacgtc gactgataac
    40681 aactacatct accctactga taacaggata aaatccgatg gccaaaaatt atgcggcgct
    40741 ggcacgctcg gtgatagcgg cactgggcgg cgttgataac atctcggcgg tcacgcactg
    40801 tatgacgcgt ttgcgctttg ttatcaaaga tgaccagctt atcgacagcc cgacgttaaa
    40861 aaccatcccc ggcgtgctcg gcgtggtacg tagtgacaac cagtgtcagg tgattatcgg
    40921 caataccgtt tcacaagcct ttcaggaagt cgtcagcctg ctgccgggag atatgcagcc
    40981 cgcacagccc gtgggtaaac ccaaactcac gctgcgtcgc attggtgcgg ggatcctcga
    41041 tgcgctgatc ggcaccatgt caccgctgat cccggcgatt atcggcggat cgatggtcaa
    41101 actgctggcg atgatcctcg agatgagcgg cgtgctgaca aaaggatcgc cgaccttaac
    41161 cattctgaat gtgattggtg acggtgcttt cttcttcctg ccgctgatgg tcgcggcatc
    41221 tgccgccatc aaatttaaaa ccaatatgtc gctggcgatt gccatcgcgg gtgtgctggt
    41281 acatccgagt tttattgaac tgatggcgaa agcggcccag ggtgaacatg tcgaatttgc
    41341 cctgattccg gtcaccgcgg tgaaatacac ctacacagtg atcccggcgc tggtgatgac
    41401 ctggtgcctg tcatatatcg aacgctgggt ggatcgcatt acaccggcgg tgaccaaaaa
    41461 cttcctcaag ccaatgctga ttgtgttgat tgccgcaccg ctggcaatcc tgctgattgg
    41521 cccgattggt atctggatcg gtagcgccat ttcggcgctg gtttacacca ttcatggtta
    41581 tctgggctgg ctttcagtcg ccattatggg cgcgctgtgg cctctgctgg taatgaccgg
    41641 gatgcaccgc gtctttacgc caaccatcat tcagaccatt gccgaaaccg gcaaagaagg
    41701 gatggtcatg ccgtcagaga tcggcgctaa cctgtcgctg ggcggttcat cactggcggt
    41761 ggcgtggaaa acgaaaaacc cggaactgcg ccagacggcg ctggctgcgg cggcatcagc
    41821 cattatggcg gggatttccg aaccggcgtt atacggcgtg gcgatccgcc tgaaacgtcc
    41881 gcttatcgcc agtcttatca gcggttttat ttgcggcgcg gttgccggta tggcggggct
    41941 tgccagccac tcaatggcag cgccagggtt atttaccagc gtgcaattct tcgatccggc
    42001 gaatccaatg agcatcgtct gggtgttcgc ggtcatggcg ctggcggtgg tgctgtcgtt
    42061 tatcctcaca ctgttgctcg gctttgagga tattcctgtt gaggaagcgg ctgccgaggc
    42121 gcgaaagcat cagagcgtac aaccgaccgt cgccaaagaa gtaagtctta attgaggatg
    42181 aaaatgtcag tatttccaga aggtttttta tggggcggcg cgcttgccgc caaccagtct
    42241 gaaggtgcgt tccgtgaagg tggcaaaggg ctgaccactg tcgatatgat cccacacggc
    42301 gagcatcgaa tggcggtgaa actggggctg gaaaaacgtt ttcagttgcg cgatgacgag
    42361 ttttatccca gccatgaggc gacggatttt tatcatcgtt ataaagaaga tatcgccctg
    42421 atggcagaga tgggattcaa ggttttccgt acctcaattg cctggagccg tctctttccg
    42481 cagggcgatg aaatcacgcc caatcagcag ggcattgctt tttatcgttc tgtctttgaa
    42541 gagtgtaaaa agtacggtat cgaaccgctg gtcacgttgt gccacttcga tgtgccgatg
    42601 catctggtca ccgaatatgg ctcctggcgt aaccgcaagc tggtggagtt tttcagccgc
    42661 tacgcccgga cctgctttga agcatttgat ggtctggtga aatactggct taccttcaat
    42721 gaaatcaaca ttatgttgca tagcccgttc tccggcgcgg gtctggtgtt tgaagaaggt
    42781 gaaaatcagg atcaggtgaa atatcaggcc gcgcatcacc agctggttgc cagtgcgcta
    42841 gccaccaaaa tcgcccatga ggttaacccg caaaatcagg tgggctgtat gctggcgggc
    42901 ggtaacttct acccttacag ttgcaagccg gaagatgtct gggcggcgct ggagaaagac
    42961 cgggaaaacc tgttttttat cgatgtgcag gcgcggggca cgtatccggc ttactctgcc
    43021 cgcgtattcc gcgaaaaagg ggtaaccatc gacaaagcac cgggcgatga tgaaatcctg
    43081 aaaaacaccg tcgattttgt ctctttcagc tattacgcct cgcgctgcgc ctcggcggag
    43141 atgaacgcca acaatagcag tgcggcgaac gtagtgaaat cgctgcgtaa cccgtatctg
    43201 caagtgagcg actggggctg gggtattgat ccactcggtc tgcgtatcac catgaacatg
    43261 atgtacgacc gttatcagaa gccgctgttt ctggtggaaa acggcctcgg cgcaaaagat
    43321 gaacttgctg ccaatggcga gattaacgat gactatcgca tcagctattt acgcgaacat
    43381 atccgcgcaa tgggcgaagc gattgcagat ggcattccgc tgatgggcta caccacctgg
    43441 ggctgtattg atttagtctc cgcctctacg ggtgaaatga gcaaacgcta cggttttgtc
    43501 tacgtagacc gtgacgacgc aggcaacggc acgctgacgc gcacacgtaa gaaatcgttc
    43561 tggtggtata aaaaagtgat tgccagtaat ggggaagatt tagagtaggt aacagtgccg
    43621 gatgcggcgt gaacgcctta tccggcctac atgtcgcacg aaccaggaag gcccgataag
    43681 cgcagcgcat cgggcaatct ggggtttggc atcagtcaaa atcaaagaaa ccattccggc
    43741 ttaacgccaa atcgggctga aagtgcctta atgtgggata tcgtcaggga tcgttgtcca
    43801 gacaaaatct gactgaccag ggatttagag ccgatttcgt tcttcaaatc cgcataggtc
    43861 agattatgtt gatcaatcag cgtgcgaagt aacgccacgc caacaggcat tgccgcaacg
    43921 gctttgtcga actctgcaaa ctcttggttg ttgttttcat attctgcaat gcggctcgcc
    43981 agaaagtcga taagtggatt ggtgtcatct tcttcaatca aatattcgac cagttccagt
    44041 gcttcacgat aatcatcttc tgagtcacta cctcccagaa aggggactgc attgaccagt
    44101 tctcttgttg ctttaacagc gcgtgcagca ttagcagtca ttctttgttc tctctgtagt
    44161 aacgtgtcag tttgtcatac tcggcgtgat tagttatgtg cttcacaaag aaccgcttat
    44221 taacaaagtt tatgtaggca atcaccctta ggttgttccc acctacatcc agaacgtacc
    44281 atttgttgcg atatttaaaa ttgtcgaggt tgggaaacgc cgttcgcatc tcttctggcg
    44341 tagaaaaatc cgtttcctta ataacccgat acaatgcctg aagagccaga gcgtcgttag
    44401 gatatttacg cgcagactct tcaaatggcg ctttagaaat gatgtgcatt tttaagtttt
    44461 aacttcctta tagtaagtaa acagccacat ctgcgagttc tatccttatg tgttcggaaa
    44521 ctatagctca tcaaatccaa gttcacaata tgtaaacact tgagcgcgat ataattaccc
    44581 ccaaaatcct catagccact ctgcaaaaag tcagttctgg ttcaccccat caagaacatc
    44641 cccctttatc aagcaacgct gcgtctgcgc cagatcttcc gtttgcgggt cgcgcccggc
    44701 gatgggtttg aggacgagtt tacggttggg ggtataagta aaacgcgtga gcgcgggata
    44761 gcttaacttg ccaaaccagc cgtgaacacg ctgccatttg gcgtctttta cctgatacag
    44821 actggttatc cagtaaccgg aatcaaactg catatccagc aattgcgtgt gaccattgcc
    44881 ttgcaaatcc agcaaatcat ctacgccagt tttgcttgcg gtgtagaatc ccctgggttc
    44941 aaagacacag ggacggcctt cgcgcgtgaa cgtcataaga attaaatgtg cataaggagc
    45001 cagaccatta ccggtacttg gtaaccagat aaccagatcc tgaatgccgt tacgatcaag
    45061 atcggcgcga tagagttgcc ctttgcccgc tatgtttgcc agcataccca caggcacaga
    45121 ccaggattta ccggcgcgat ctttaccgtt caattgccat tcactatcat catttggcat
    45181 cttttcaagc gtcacgccag caacgcccac ttttctaaaa tgccaggttt gctcggcatc
    45241 ataaggcatg gtcaggagag gtttcagcgc ccgcttttgc acgtattcgc tgtcactcat
    45301 tggcagatat tcggtaaagc tggtggcctg tgcaggcagc actaagctca ataaaaaagc
    45361 gcacatccct gttctgatat tcatttcgct gaattccctg atggagaatt attcttcttc
    45421 caccgctaac tgcgcgaagc cgccattccc ttcccagcct tccagtcgtt gataaacggt
    45481 ttctaccgca tctttaatcg gctgagtcat cgggtagtaa aagccgacga tatccggctg
    45541 gatgccgagg aaaatcactt cgccaatgtc ttctttcaac tggtcgataa gatagttcag
    45601 cggcatgtta tgggtagtca tcataaacat ctcggcgata tcatccgggt cgatgatgcg
    45661 gatttcgccg gggtttagcc ccatatcagt agcgtcaaca atcagtaacc gcgtagggcg
    45721 cagttcacgg atggcgacga tgtcgttttc cggtgcacta ccgccatcaa tcaccaccca
    45781 gttacctttc ggcgcggcgg cgcacttttc cgccagcagc gggccagcgc catcatcgcc
    45841 catcatgcta ttgccaacac agagtaaaac gtcagtcacg cagtctcctc accatcaaat
    45901 agatggcgtt ttcctgatga atatcatgca gcatacttaa cagcattgta ctccattcct
    45961 tctgctgcga agtttgcgct gcccgggctt tatcgaatgc gttggccagc atcggcacat
    46021 ggttaatgtc gatgacgatc tcaccatatt tcggcacgcc ttccattttg cggcgggctt
    46081 cactgcccgc ctccagagtg gcaatccatg ccagatattc atcccacggg caggtgagcg
    46141 ccgcttccag gcaatcgata accccaaggt ggtgaccaat cgccaggctg taatagacca
    46201 cctgctgcgc ctcggcgggc gtggcatcgt tctcatcaat aaatttacgg ctcagttgac
    46261 tgaacaccac cttttcactc atcggatacg cgcctcttca acaacatgat tcagatggct
    46321 gacaatctcg ttcagacgcg gatcgttttc cgcttccagc cagcgtgcaa cctgttcttc
    46381 gccctgccct aactgtgtca ggaagtcatc ggcaatctga cgaccgtaac gataacccgc
    46441 caggcggcgt gcttcgcgat ccactttcac acgcagcggc tgcaccatat cgccatgcag
    46501 gatttccgcc tgttgttcat cctgctcacc cgggccacgg gcgtgaattt tctgctccag
    46561 caggccgagc gccattgcaa agccgtacag cgtggcggca ggcgttggcg ggcaaccagg
    46621 aatgtagaca tctaccggga cgattttatc cgtaccgccc cacacgcagt agagatcgtg
    46681 gaaaatacca ccgctgttac cgcaggcacc gtaggagata caaattttcg ggtccggcgc
    46741 ggactgccac gcacgcagcg caggggatcg cattgcacgg gtgaccgcgc cggtaaacag
    46801 taaaatatcc gcatgacgcg gtgaaggaac gactttaatg ccgaagcgtt ctgcatcaaa
    46861 cagcggcgaa agcgtggcga aaatttcgat ttcgcagccg ttgcagccgc cgcagtccac
    46921 gcgataaacg taggcagaac gtttgatttt tttcagtaac gacgccttca tgctggcgat
    46981 ggattcatcc accgtcatcg ggaccggaat gccgttggcg tcacgggggc ctaataaatt
    47041 gctcatcaga tggcctcttt catatggcga gtcagttcaa tacggtcgga cggcaccagg
    47101 catttctggc gcttacattc cgggcaagtc tcaaagcttt cgcggtggtt ttccgcgcgg
    47161 ctgtcgccgt tgtgcttaag cagcgcaatg gcgtagtcga tctctttctg gacggcgaaa
    47221 ggacgattgc agacgcggca gttgcacagc gcgaagcggg actgttgcag gaagtcttct
    47281 ttcttccaca ctgccagttc gtactcttgc gacagtttga tcgccgccgt cgggcaaact
    47341 tcttcgcagc gtccacagaa gatgcagcgc ccgagattaa actcccacgc cagctcaccg
    47401 gtggcgaggt cagtttcaac cgttaaggcg tttgacgggc aggcattgac gcaggccgca
    47461 cagccgatgc actgctgcgg gttctgctcc ggcttaccac ggaagttttt atcaaccgca
    47521 atcggctcca gcggataaga cgaggtcgcc gtgccggttt tgatgacttt tttgataaag
    47581 gtaaacatgg cgattcctta tttcagcggc gagtttttac gctcaatgct gtaacgctcg
    47641 agttctttgt acggcaccac tttgctcttt ttcttacgca catcgaccac ggtcatgcgg
    47701 tcggtacagg agtagcaagg gtccaagcta ccgataatca gcggcgcatc ggaaacggtg
    47761 ttaccgcgca gcatgtagcg cagggtcggc cagttcgcgt aggtcgcggc acggcagcgc
    47821 cagcggtaca gcttctggtt gtcgccggtc atgctccagt ggatatcgtc gccgcgcggc
    47881 gcttcggcaa agcccagcgc gaaacggtgc ggaatgtagg taaagccttc caccatcagc
    47941 ggaccgcccg gcaggttatc cagaccgtag tcgatcatgt tcagcgcggt ataaacttcg
    48001 ttgatacgca ctttcagacg ggaaataacg tcgcagccct gctcgctgtg gacttccatt
    48061 ggcagcaggc cataaccgac aaacgggtga tcggcgcgag tatcgcgggc gtgaccgctg
    48121 gcgcgaacca tcgggccaac gttactgaaa tcgcgggcca tttccgggtc cagacgacca
    48181 atgccgacgg tgcgctgttc catgttcggc gtactcagca gcacatccac cagctcctgc
    48241 acttcacgac gcatctgttg cgccagctgg cgggtctgga tcatgtcatc tttcaacaga
    48301 tcgcgacgaa tcccgccgat caggttcagg ccgtaggttt tacgcgcacc ggtgaggatc
    48361 tctgccattt tcatggaggt ttcacgcacg cggaagaact gcataaagcc ggagtcaaag
    48421 ccggtaaagt ggcaggcgag accgaggttg agcagatgca agtgcaggcg ttccacctcc
    48481 agcagaatgg cgcggatcat ctgcgcacgt tctggcacct gaatacccat cgcgttttct
    48541 accgacgtgg tgtaggcggt gctgtgggca aagccgcaga tcccgcacac acggtcagag
    48601 aggaaggtca cttcgttgta acccatacgg gtttccgcca gtttttccat gccgcgatga
    48661 acgtagaaca gacggtagtc ggcgtcgata atgttttcgc catcgacgaa cagacggaag
    48721 tggcccggtt cgtcagaagt gacgtgcagc ggaccaatcg gcacgacgtt gtttttcttg
    48781 tcgcccagtt cgttgatgaa ctcgtaggtt tcagcatcgg tggtcggtgc cggacgctga
    48841 cgataatcca tgctgtcttt acgcagcgga taaagttcat ccggccagtc atccggcagc
    48901 accagacggc gttcatccgg cagaccaacc ggaatcaaac cgtacatatc gcgcacttcg
    48961 cgctcgcccc acaccgccgc cggaacgcgc ggcgtcacgg acggatactc cggtttgttg
    49021 gcatcaactt cgacgcgaac cgttacccag cacttagtcc ccttctccat cgacagcacg
    49081 taataaacgg cgtaatgacc attcagtttg cgttcgtcgt taccaaaaag caccgacagc
    49141 cagcccccct gcttgtagta gagaaactcc accacttccg gcagatagtt cacctttacg
    49201 gtgacagtca gctgatcttt ggtctgccag gcgtggtcca gcacgacgcc cggaaatgcc
    49261 tcattcagcg cggcgagata atgttgacct aatttttctt cagacatgct caaactctct
    49321 ttaatcacgc cgccagcaag gagacgaacg ctaaaaatgc aaagccaaac ccagtccagg
    49381 taatgcgcgg agtaatatca agacgcagac gggccatgct gttttcgaac agcgcgataa
    49441 ccaggacgcc gacgaccagt ttgacgatgg caatcaccag cgccagcagc agcccaccgg
    49501 cggtgaaggt ttccatctgt ccccacggga taaatacgcc gacgaacatc tgcagcacca
    49561 ccagctgttt caggctgata ccccatttca tgacgccaaa gccgctgccg ctgtattcag
    49621 agagcgggcc ttcctgcagc tcctgctcgg cttccgccag gtcgaacggc agtttgccca
    49681 tttcgataaa ggtggcgaac gcacaggcac aaagcgccag taccagcagg atgctctggc
    49741 tcagcggcca gtgataaacg gtgtcggtga tgttgctgat gttggtggaa ccggcaacct
    49801 gtgcggcaac ccacagacca agcagcagca tcggttcgac cagcacgcca agcatcgctt
    49861 cacggctcgc gccgatagcg gtaaacgggc taccggtatc cagaccagaa atggcaaaga
    49921 agaaacgcgc gatggcaaag agatacagta aggtgattaa atcacccagt tgcggcagcg
    49981 gagaaccgac ggtcaccacc ggcagcgcag tagcgatagt cagcatgacg cccaccatca
    50041 catacggcgt caggcggaac acccagccgg aggcatccgg gccaacgctc tggcggccca
    50101 gcagtttgaa aatgtcacga tactcctgca acacacccgg cccacgacgg ttatgcagac
    50161 gggcgcgcgc cacgcgggta ataccggaga gcagcggcgc aacggcaaat aacaccagcg
    50221 cctgaattaa cggatataaa acactcattc tcaggctcct cgtgaaacaa taatcaccac
    50281 cagtaccgcc agttcaacca gcgccatccg gcggaacagc aacgcactac cttcgcactg
    50341 ccagcccggc accagcgaca ccggattcag ccatttgcgc agtttcagca ctggcgcaaa
    50401 cgcctgttta accggcatgg caaaaccgtg agcggtaatc accattgatt tttcatggtc
    50461 gtagccgcac acccaggccg caccgcggga acgtgacggc aggcggtcgc ctttgcaaat
    50521 cgccataatg atgaatggca gcagcgggca ggcaatcagc agcaatgtga tcattggttg
    50581 agaaacggtg gtgttagcag gctccagagg cagaggtaca gcagcagaga gcatcggcag
    50641 cagccacggc gcagcaacac cgccaattac gcagcaaatc gccagtgcca ctacgcttac
    50701 gctcatcagg agcggcgcac aggtggcgtt ttcggcttct ttggtacgcg gcgcgccgag
    50761 gaacgtgacg ccatagactt tcgccataca catcaccgcc agcgcaccgg taattgccag
    50821 acccacagcg agcagcggcc ccagcagacg ggcaacaaac gcgccgctat tgctcagttt
    50881 gaagaaggat tgatagataa cccattcccc ggcaaacccg ttcagtggcg gcagcgcagc
    50941 cattgccatc aacccgacta acatggcgat ggagataacc ggcattttct tgccaatacc
    51001 accgagtttt tcgatatcgc gatgaccggt acggaaccag acgctccccg ccccgaggaa
    51061 cagtacgctt ttgaacaggc tatggttaag cagatggtac agaccaccga ccaggccaag
    51121 agcaatcagc gccggttgtt cgagcgcgat acccgttacg ccagcgccca gccccagcag
    51181 tgacagggtt aacatgccca gcaggccaat tttcatgact accgtagaaa acaacgcggc
    51241 agctggcgca gaggcgttag catgtgcctg cggcacccag ccgtgcagcg gaataatccc
    51301 ggccagcagg ccaaagccaa tcacaccgag cagccagata tcggaaccga gcggcagctg
    51361 ttgcatacgc atatccagta ggcgcagatc cagcgtgcca taacgctgcc acagcagcca
    51421 gcaagcaatc accagcagca gagtgccaag acgccccagc gcaaaccaca gtttgccctc
    51481 tttgctgttg ctggtgagga acaccgcaca caaggccatg acttcggcca ttaccacgaa
    51541 catgccgagg ttgctggcga tgacggcgca gacggcggca gccatcaaca tattgatctg
    51601 caagccgttg cacttcacct gcgcgtggcg atgccagtca atgttgtaga ggctgacaaa
    51661 cagaccgcac agaccgagcg taatcagcca aatcgcgtta agcggagaga tttgcacatc
    51721 gtagcttacc agcgacagcg caccgctcac gccaaccgcg ccagtcagta cagtgaagcc
    51781 cgcggctgcc gtatacagac taccaaccgc gccgccaatt ccagctatcc agccacttaa
    51841 cgctttttga aaagaaaaga gaaatgccag aacagcggcg gcgacaaacc atgccacgcc
    51901 gctattgatc agggaaattg cgctcattta gcctctccac tttgagcctg ctgaaacaag
    51961 gtgagatcgc caaagtccgt gttaaaggtc agctcacgct tacgtttgct gacgcgggcg
    52021 atatcggtgt tatccaccag atgcagggct ttagtcgggc acatccgcac gcaggccgga
    52081 ccttgttcat caaaactgca aaggtcacat ttgacggcga tcgcgcgaat acccggcacc
    52141 cagtcaagca gcgtactgat acgcgccgga gcaggcggtg ccggtggcgc tttcggggta
    52201 ttggcgtttg ccggaatatc cagcggacgg ctgccggaaa attcaattgc gccaaacggg
    52261 caggcgatgc cgcacagttt gcagcttacg cacagacttt cattcaactg cacggcccca
    52321 tcaatgcggg tgatggcgtt aaccgggcaa accaccgcac agggtgcatc ttcacagtgg
    52381 tgacagagct gcggcgcaga ttctttttca ttcagcatca ctctcaggcg cggcattgat
    52441 tgcaggccgt gctggcgatg cgtctctgaa caggcggcct cacaagtgtg gcagccgata
    52501 cagagcgtgg agtcaacaat tacaaaacga ttcaccaggc attcctcagg tgattgtcat
    52561 ttttgacgaa aacatgccga tgaaatgtca ttttcgacac tcatcgacac gcccatcccc
    52621 aaacaggcgt aacgcctgca aaacgggcaa agcctcagct catgctgccg ggctttgtcc
    52681 ctttaccagt tggcttaaat tcaccggcac attgttttca atagcggtgt ataagctgtc
    52741 gtaaacgcca gcgatttttt cgagatagtg ttccagaacc tgttcgcgat cgcggatgtt
    52801 gaccttgcca tcaatcatgc cgtcaatgct cagttgcgcc tgcgcaatcc gctgtttgtc
    52861 ttcgccatct tttgtttcga cgtaatactc gatggtgtgg ctgttaaagc catccgccag
    52921 ggtgacgtaa atacgaaaat gttcaaaaag gacgaaacag agttctttgt ccggcgcgca
    52981 gctcatggcg tgatgcaggc gcgctttcga taacgtgatc ccctgaacca gcgaattgca
    53041 gtagatgtgc cactggtcct gtaggcgacg atgccgctgt gcgatgtaat cggctttctc
    53101 gcttatttcc caaatagtca ttgtcaggtt acccgtttaa cagagatgcc agctttaagc
    53161 attttctatg ccaactttta atttattgtt attaaataga tttttaagct aaagatgaat
    53221 ttcgtcgccg tgtcgacgtg tcatttcgac atcatcgaca ttattcaccg cagggataat
    53281 caacactggc acaattattg cttgtagctg gcaatagtta atgggaggcg atatgcacga
    53341 aataaccctc tgccaacggg cactggaatt gatcgaacag caggccgcaa aacacggcgc
    53401 aaaacgcgta actggggtct ggctcaaaat tggcgcattt tcttgtgtcg aaaccagctc
    53461 tcttgccttt tgttttgatc tggtttgccg cggcagcgtg gcggaaggtt gtaaactgca
    53521 cctcgaagaa caagaggccg aatgctggtg tgaaacatgc caacagtatg tgacgctact
    53581 gacccagcgc gtccgccgct gtccacagtg tcatggtgac atgctacaga ttgtggcaga
    53641 cgacggttta cagattcggc ggatagaaat agaccaggag tgagcgatgt gtacaacatg
    53701 cggttgcggt gaaggcaacc tgtatatcga gggtgatgaa cataaccctc attccgcgtt
    53761 tcgtagcgcg ccatttgccc cggcggcacg cccgaagatg aaaatcaccg gcattaaagc
    53821 gcctgaattt acccccagcc agactgaaga aggcgacctg cattacggtc atggcgaagc
    53881 gggcactcac gcgccgggca tgagccagcg tcggatgctg gaagtcgaaa ttgacgtgct
    53941 ggacaaaaat aaccgtctgg ctgaacgcaa ccgcgcgcgc tttgctgccc gcaagcaact
    54001 ggtgctcaac ctggtttcca gccctggttc cggtaaaacc accctgctga cggaaaccct
    54061 aatgcgcctg aaagacagcg ttccgtgcgc ggttattgaa ggtgaccagc aaaccgtgaa
    54121 cgatgccgca cgcattcgcg ctaccggcac accagcgatt caggtgaaca ccggtaaagg
    54181 ctgccatctt gacgcacaga tgattgccga cgccgcaccg cgtctgccac tggacgataa
    54241 cggtattctg tttatcgaaa acgttggcaa cctcgtatgc ccggccagct tcgatctcgg
    54301 tgaaaaacac aaagtggcgg tgctttccgt taccgaaggt gaagacaaac cgctgaaata
    54361 tccgcatatg tttgccgccg cctcgctgat gctgctcaac aaagttgacc tgttgccgta
    54421 tctcaacttt gacgttgaga agtgcatcgc ctgcgcccgc gaagtcaatc cagaaattga
    54481 aatcatcctt atttccgcca ccagcggcga agggatggac cagtggctga actggctgga
    54541 gacacagcga tgtgcatagg cgttcccggc cagatccgca ccattgacgg caaccaggca
    54601 aaagtcgacg tctgcggcat tcagcgcgat gtcgatttaa cgttagtcgg cagctgcgat
    54661 gaaaacggtc agccgcgcgt gggccagtgg gtactggtac acgttggctt tgccatgagc
    54721 gtaattaatg aagccgaagc acgcgacact ctcgacgcct tacaaaacat gtttgacgtt
    54781 gagccggatg tcggcgcgct gttgtatggc gaggaaaaat aatgcgtttt gttgatgaat
    54841 atcgcgcgcc ggaacaggtg atgcagttaa ttgagcatct gcgcgaacgt gcttcacatc
    54901 tctcttacac cgccgaacgc cctctgcgga ttatggaagt gtgcggtggt catacccacg
    54961 ccatttttaa attcggcctc gaccagttac tgcctgaaaa cgttgagttt atccacggtc
    55021 cgggttgccc ggtgtgcgta ctgccgatgg gtagaatcga cacctgcgtg gagattgcca
    55081 gccatccgga agtcatcttc tgtacctttg gcgacgccat gcgcgtgccg gggaaacagg
    55141 gatcgctgtt gcaggcaaaa gcacgcggtg ccgatgtgcg catcgtttac tcgccgatgg
    55201 atgcgttgaa actggcgcag gagaatccaa cccgtaaagt cgtattcttc ggcttaggtt
    55261 ttgaaaccac catgccgacc accgccatca ccctgcaaca ggcgaaagcc cgcgatgtgc
    55321 agaattttta cttcttctgc cagcatatta cgcttatccc gacgctgcgc agtttgctgg
    55381 aacagccgga taacggtatc gacgcgttcc tcgcgccggg ccacgttagt atggttatcg
    55441 gcactgatgc ctataatttt atcgccagcg attttcatcg tccgctggtg gtcgctggtt
    55501 tcgaacccct tgatctactg caaggcgtgg tcatgctggt ggagcagaaa atagcggccc
    55561 acagcaaggt agagaatcag tatcgtcggg tggtaccgga tgccggtaac ctgctggcgc
    55621 aacaggcaat tgctgatgtg ttctgtgtca acggcgacag cgaatggcgc ggcttaggcg
    55681 tgattgaatc ttctggcgtg cacctgacgc cggattatca acgattcgat gccgaagcac
    55741 atttccgccc agcaccgcag caggtctgcg atgacccgcg cgcgcgttgt ggtgaggtat
    55801 taacgggcaa atgtaagccg catcaatgcc cgctgtttgg taacacctgt aatcctcaaa
    55861 ccgcatttgg tgcactgatg gtctcctccg aaggagcgtg cgccgcgtgg tatcagtatc
    55921 gtcagcagga gaatgaagcg tgaataatat ccaactcgcc cacggcagcg gcggccaggc
    55981 gatgcagcaa ttaatcaaca gcctgtttat ggaagccttt gccaacccgt ggctggcaga
    56041 gcaggaagac caggcacgtc ttgatctggc gcagctggta gcagaaggcg accgtctggc
    56101 gttctccacc gacagttacg tcattgaccc gctgttcttt cctggcggta atatcggcaa
    56161 gctggcgatt tgcggcacag ccaatgacgt tgcggtcagt ggcgctattc cgcgctatct
    56221 ctcctgtggc tttatcctcg aagaaggatt gccgatggag acactgaaag ccgtagtgac
    56281 cagcatggca gaaaccgcgc gcgcggcagg cattgccatc gttactggcg atactaaagt
    56341 ggtgcagcgc ggcgcggcag ataaactgtt tatcaacacc gctggcatgg gcgcaattcc
    56401 ggcgaatatt cactggggcg cacagacgct aaccgcaggc gatgtattac tggttagcgg
    56461 cacgctcggg gaccacgggg cgactatcct taacctgcgt gagcagctgg ggctggatgg
    56521 cgaactggtc agcgactgcg cggtactgac gccgcttatt cagacgctgc gtgacattcc
    56581 cggcgtgaaa gcgctgcgtg atgccacccg tggtggtgtg aacgcggtag ttcatgagtt
    56641 cgcggcagcc tgcggttgtg gtattgaaat ttcagaagtg gcactgccgg ttaaacctgc
    56701 cgtgcgcggc gtttgcgaac tgctgggact ggacgccctt aactttgcca acgaaggcaa
    56761 actggtgatc gccgttgaac gcaacgcggc agagcaagtg ctggcagcgt tacattccca
    56821 tccactgggg aaagacgcgg cgctaattgg tgaagtggtg gaacgtaaag gtgttcgtct
    56881 tgccggtctg tatggtgtga aacgaaccct cgatttacca cacgccgaac ctcttccgcg
    56941 tatatgctaa taaaattcta aatctcctat agttagtcaa tgaccttttg caccgctttg
    57001 cggtgctttc ctggaagaac aaaatgtcat atacaccgat gagtgatctc ggacagcaag
    57061 ggttgttcga catcactcgg acactattgc agcagcccga tctggcctcg ctgtgtgagg
    57121 ctctttcgca actggtaaag cgttctgcgc tcgccgacaa cgcggctatt gtgttgtggc
    57181 aagcgcagac tcaacgtgcg tcttattacg catcgcgtga aaaagacacc cccattaaat
    57241 atgaagacga aactgttctg gcacacggtc cggtacgccg cattttgtcg cgccctgata
    57301 cgctgcattg cagttacgaa gaattttgtg aaacctggcc gcagctggtc gcaggtgggc
    57361 tataccccaa atttggtcac tattgcctga tgccactggc ggcggaaggg catatttttg
    57421 gtggctgtga atttattcgt tatgacgatc gtccctggag cgaaaaagag ttcaatcgtc
    57481 tgcaaacatt tacgcagatc gtttctgtcg tcaccgaaca aatccagagc cgcgtcgtca
    57541 acaatgtcga ctatgagttg ttatgccggg agcgcgataa cttccgcatc ctggttgcca
    57601 ttaccaacgc ggtgctttcc cgcctggata tggacgaact ggtcagcgaa gtcgccaaag
    57661 aaatccatta ctatttcgac attgacgata tcagtatcgt cttacgcagc caccgtaaaa
    57721 acaaactcaa catctactcc actcactatc ttgataaaca acatcccgcc cacgaacaga
    57781 gcgaagtcga tgaagccgga accctcaccg aacgcgtgtt caaaagtaaa gagatgctgc
    57841 tgatcaatct ccacgagcgg gacgatttag ccccctatga acgcatgttg ttcgacacct
    57901 agggcaacca gattcaaacc ttgtgcctgt taccgctgat gtctggcgac accatgctgg
    57961 gcgtgctgaa actggcgcaa tgcgaagaga aagtgtttac cactaccaat ctgaatttgc
    58021 tgcgccagat tgccgaacgt gtggcaatcg ctgtcgataa cgccctcgcc tatcaggaaa
    58081 tccatcgtct gaaagaacgg ctggttgatg aaaacctcgc cctgaccgag cagctcaaca
    58141 atgttgatag tgaatttggc gagattattg gccgcagcga agccatgtac agcgtgctta
    58201 aacaagttga aatggtggcg caaagtgaca gtaccgtgct gatcctcggt gaaactggca
    58261 cgggtaaaga gctgattgcc cgcgcgatcc ataatctcag tgggcgtaat aatcgccgca
    58321 tggtcaaaat gaactgcgcg gcgatgcctg ccggattgct ggaaagcgat ctgtttggtc
    58381 atgagcgtgg ggcttttacc ggtgccagcg cccagcgcat tggtcgtttt gaactggcgg
    58441 ataaaagctc tctgttcctc gacgaagtgg gcgatatgcc actggagtta cagccgaagt
    58501 tgctgcgtgt attgcaggaa caggagtttg aacgtctcgg cagcaacaaa atcattcaga
    58561 cggacgtacg tttaattgcc gcgactaacc gcgatctgaa aaaaatggtc gccgaccgag
    58621 agttccgtag cgatctctat taccgcctga acgtattccc gattcacctg ccgccactac
    58681 gcgagcgtcc ggaagatatt ccgctgctgg cgaaagcctt taccttcaaa attgcccgtc
    58741 gtctggggcg caatatcgac agcattcctg ccgagacgct gcgcaccttg agcaacatgg
    58801 agtggccggg taacgtacgc gaactggaaa acgtcattga gcgcgcggta ttgctaacac
    58861 gcggtaacgt gctgcagctg tcattgccag atattgtttt accggaacct gaaacgccgc
    58921 ctgccgcaac ggttgtcgcc caggagggcg aagatgaata tcagttgatt gtgcgcgtgc
    58981 tgaaagaaac caacggcgtg gttgccgggc ctaaaggcgc tgcgcaacgt ctggggctga
    59041 aacgcacgac cctgctgtca cggatgaagc ggctgggaat tgataaatcg gcattgattt
    59101 aactgcaaat tgccggacag atctgcctgt ccggcatact attcacgagg ttttttcgga
    59161 cgatattttt ccggcagttc tggtaccgga cgcttgtcat cgatgagatg acgcacggtt
    59221 aagatcggat gacgccacag cattctcggc ccggcccaac gcataatctg tttcatctct
    59281 tcacgctttg caggctggta acagtgcacc ggacactgct tacaggctgg tttctcttcg
    59341 ccgaacacac atttatccag ccgcttttgc gcgtaaacaa acaacgcctc gtaatgctcc
    59401 ggctccgctg acgcctgcgg gcatttcgct tgataaagat cgatcatttt tttaatcgtc
    59461 agtttttcac gagagatacg cttgccggac atgctgcctc catactctta aggtgtattt
    59521 atattacaac ttaatttgaa aggggactat ggctctgaag gaggaggttt attcaaccga
    59581 cgcaattttg cgtacttgct tcataagcat cacgcaaaag ctgcaaaaca gcatctttcc
    59641 cggaaccagc atcaagaact cgccgttcgc ttcttcccct gaaatgatta actccggtat
    59701 catgtgcgcc ttatgtgatt acaacgaaaa taaaaaccat cacaccccat ttaatatcag
    59761 ggaaccggac ataaccccat gagtgcaata gaaaatttcg acgcccatac gcccatgatg
    59821 cagcagtatc tcaggctgaa agcccagcat cccgagatcc tgctgtttta ccggatgggt
    59881 gatttttatg aactgtttta tgacgacgca aaacgcgcgt cgcaactgct ggatatttca
    59941 ctgaccaaac gcggtgcttc ggcgggagag ccgatcccga tggcggggat tccctaccat
    60001 gcggtggaaa actatctcgc caaactggtg aatcagggag agtccgttgc catctgcgaa
    60061 caaattggcg atccggcgac cagcaaaggt ccggttgagc gcaaagttgt gcgtatcgtt
    60121 acgccaggca ccatcagcga tgaagccctg ttacaggagc gtcaggacaa cctgctggcg
    60181 gctatctggc aggacagcaa aggtttcggc tacgcgacgc tggatatcag ctccgggcgt
    60241 tttcgcctga gcgaaccggc cgaccgcgaa acgatggcgg cagagctgca acgcactaat
    60301 ccggcggaac tgctgtatgc agaagatttc gccgagatgt cgctgattga aggccgtcgc
    60361 ggcctgcgcc gtcgcccgct gtgggagttt gaaatcgaca ccgcgcgcca gcagttgaat
    60421 ctgcaatttg gcacccgcga tctggtcggt tttggcgtcg agaacgcgcc gcgcggactt
    60481 tgtgctgccg gttgtctgtt gcagtatgcg aaagataccc aacgcacgac cctgccgcat
    60541 attcgttcta tcaccatgga acgtgagcag gacagcatca ttatggatgc cgcgacacgt
    60601 cgtaacctgg aaattactca gaacctggcg ggcggtgcgg aaaatacgct ggcttctgtg
    60661 ctcgactgca ccgtcacgcc gatgggtagt cgtatgctga aacgctggct gcatatgcca
    60721 gtgcgcgata cccgcgtgtt gcttgagcgc cagcaaacta ttggcgcatt gcaggatttc
    60781 accgccgagt tgcagccggt actgcgtcag gtcggcgacc tggaacgtat tctggcacgt
    60841 ctggctttac gaactgctcg cccacgcgat ctggcccgta tgcgccacgc tttccagcaa
    60901 ctgccggagc tgcgtgcgca gttagaaact gtcgatagtg caccggtaca ggcgctacgt
    60961 gagaagatgg gcgagtttgc cgagctgcgc gatctgctgg agcgagcaat catcgacaca
    61021 ccgccggtgc tggtacgcga cggtggtgtt atcgcatcgg gctataacga agagctggat
    61081 gagtggcgcg cgctggctga cggcgcgacc gattatctgg agcgtctgga agtccgcgag
    61141 cgtgaacgta ccggcctgga cacgctgaaa gttggcttta atgcggtgca cggctactac
    61201 attcaaatca gccgtgggca aagccatctg gcacccatca actacatgcg tcgccagacg
    61261 ctgaaaaacg ccgagcgcta catcattcca gagctaaaag agtacgaaga taaagttctc
    61321 acctcaaaag gcaaagcact ggcactggaa aaacagcttt atgaagagtt gttcgacctg
    61381 ctgttgccgc atctggaagc gttgcaacag agcgcgagcg cgctggcgga actcgacgtg
    61441 ctggtgaacc tggcggaacg ggcctatacc ctgaactaca cctgcccgac cttcattgat
    61501 aaaccgggca ttcgcattac cgaaggtcgc catccggtag ttgaacaagt actgaatgag
    61561 ccatttatcg ctaacccgct gaatctgtcg ccgcagcgcc gtatgttgat catcaccggt
    61621 ccgaacatgg gcggtaaaag tacctatatg cgccagaccg cactgattgc gctgatggcc
    61681 tacatcggca gctatgtacc ggcacaaaaa gtcgagattg gacctatcga tcgcatcttt
    61741 acccgcgtag gcgcggcaga tgacctggct tccgggcgct caacctttat ggtggagatg
    61801 actgaaaccg ccaatatttt acataacgcc accgaataca gtctggtgtt aatggatgag
    61861 atcgggcgtg gaacgtccac ctacgatggt ctgtcgctgg cgtgggcgtg cgcagaaaat
    61921 ctggcgaata agattaaggc attgacgctg tttgccaccc actatttcga gctgacccag
    61981 ctaccggaga aaatggaagg cgtcgccaac gtacatctcg atgcgctgga gcacggcgac
    62041 actatcgcct ttatgcacag cgtgcaggac ggcgcggcga gcaaaagcta cggtctggcg
    62101 gttgctgctc tggcgggcgt accgaaagag gttattaagc gcgcacggca aaaactgcgt
    62161 gagctggaaa gcatttcgcc gaacgccgcc gcgacgcaag tggatggtac gcagatgtct
    62221 ttgctgtcag tgccagaaga aacttcgcct gcggtcgaag cactggaaaa tctcgacccg
    62281 gattcactga ctccgcgtca ggcgctggag tggatttatc gcttgaagag tctggtgtaa
    62341 taacaattcc cgatagtctt ttgctatcgg gaatattaac gacaactgac gaatcaaata
    62401 aaaataccct gtataatagg aaagcttatt ttacagggta aaaccatgcc atctacacgc
    62461 tatcaaaaaa tcaatgccca tcactatcgc catatatggg tcgttggtga tattcatggt
    62521 gaatatcagt tattacaatc ccgttaacat caactctctt tttaccctga aaccgactta
    62581 cttatttcca caggcgataa tattgatcgt gggccgaaga gtcttaacgt gctgcgcctg
    62641 ctaaaccaac cctggtttac gtcggttaaa ggcaaccacg aagcgatggc gttggatgca
    62701 ttcgaaactg gcgatggaaa tatgtggctt gccagcggtg gtgactggtt tttcgattta
    62761 aatgattcag agcaacaaga ggcaatagat ctgttgctga aattccatca ccttccacat
    62821 attattgaaa tcattaatga caacataaaa tatgccatcg cacatgcaga ttatccgggt
    62881 gatgaatatc agtttggtaa agaaatagca gaaagcgaat tactctggcc tgttgatcgt
    62941 gtgcagaaat cgcttaatgg cgagttacaa caaataaacg gcgctgatta ttttatattt
    63001 ggacatatga tgtttgataa cattcagacg ttcgctaacc agatttatat tgataccggc
    63061 acgccgaaaa gcgggcggct gtcattttat aaaataaagt agtctcatgc ttcttctgtg
    63121 aagcatgagt aaccaaatgt tattgcaggc cattattcat ttttcgctac cagcaaagag
    63181 agatcctgct tcgccagcgc gcgactggca ctctccggca aaccgtcgtc tgtgataatc
    63241 tgatcaaact cgcttaatgg taacgccagc catgtcgcca cctgaccata tttcgtcgca
    63301 tcacagacca aaactcgctg gcggctggca ctggcaatcg cccgtttcac cgtgacttta
    63361 tcttccgctg gcgtagaaat cccccgcaca ctccatgacg atgcagaaat aaaagcctga
    63421 tcaatcatca ggctgcgcag catggtcgca gcggcttccc cgacacagga acggttttcc
    63481 cgacacactg caccgccagt gtgaataatt gtgcaattac tgttgtcgag caagtagttc
    63541 gcaataacga aatcgtttgt gaccacagtc agtgactcca tgtgaatcag atgctgtgct
    63601 atcgctaacg tggtcgttcc cgcatccaga tagatacaac ttcccggctg aacaagactt
    63661 gccgccagct tgccaatagc cgctttttgc gtcattgcca gcgcagtttt tacctgatga
    63721 gaaggttcat gcgccacgcg tcccggagac tggacgcctc cggacaccag cacaacggct
    63781 ccctgctgct ccagtttttg taaatcccga cgaatggtca tatgtgacac attcattctg
    63841 tccgttagtt cagcaatact gacaatgcct ttttcagcta ccatctcaag gatgatttgg
    63901 cgacgctcta cgggtatcaa cttttgctcc ttcctttgtc ttgctgacat tctacgctat
    63961 ttgcctgcga aacgtgcgcg gcgcaactaa cgcttagttc acataaaata acacgcaatg
    64021 ttaatttatg tgaatcagct caccatatcg ttatcttcca gcgcttatat tcacaatatc
    64081 aaacaaaata tcacttaaat taacaatgag agcagatgaa aacgggatct gagtttcatg
    64141 tcggtatcgt tggcttaggg tcaatgggaa tgggagcagc actgtcatgt gtccgcgcag
    64201 gtctttctac ctggggcgca gacctgaaca gcaatgcctg cgctacgttg aaagaggcag
    64261 gtgcttgcgg ggtttctgat aacgccgcga cgtttgccga aaaactggac gctctgctgg
    64321 tgctggtggt caatgcggcc caggttaaac aggtgctgtt tggtgaaaca ggcgttgcac
    64381 aacatctgaa acccggtacg gcagtaatgg tttcttccac tatcgctagt gctgatgcgc
    64441 aagaaattgc taccgctctg gctggattcg atctggaaat gctggatgcg ccagtttctg
    64501 gtggtgcagt aaaagccgct aacggtgaaa tgactgtcat ggcctccggt agcgatattg
    64561 cctttgaacg actggcaccc gtgctggaag ccgttgccgg aaaagtttat cgcataggtg
    64621 cagaaccggg actaggttcg accgtaaaaa ttattcacca gttgttagcg ggcgtacata
    64681 ttgctgccgg agccgaagcg atggcacttg cagcccgtgc ggggatcccg ctggatgtga
    64741 tgtatgacgt cgtgaccaat gccgccggaa attcctggat gttcgaaaac cggatgcgtc
    64801 atgtggtgga tggcgattac accccgcatt cagccgtcga tatttttgtt aaggatcttg
    64861 gtctggttgc cgatacagcc aaagccctgc acttcccgct gccattggcc tcaacagcat
    64921 tgaatatgtt caccagcgcc agtaacgcgg gttacgggaa agaagacgat agcgcagtta
    64981 tcaagatttt ctctggcatc actctaccgg gagcgaaatc atgatcaaga ttggcgttat
    65041 cgccgatgat tttaccggcg cgacggatat cgccagtttt ctggtggaaa acggtctacc
    65101 aacggtacaa attaacggtg ttccaacagg taaaatgccg gaagcaatcg acgcactggt
    65161 gatcagcctg aaaacgcgct cctgtccggt ggttgaagcc acacagcaat cgctggcggc
    65221 tctgagctgg ttgcaacagc aaggttgcaa acagatctat ttcaaatact gctctacttt
    65281 cgacagtacg gcgaaaggta atattggccc ggttaccgat gccttaatgg atgctctcga
    65341 cacgccgttt acggtcttct ctccggccct gtcggtcaac ggacgtacgg tttatcaggg
    65401 gtatttgttc gtaatgaatc aactgctggc cgaatccggg atgcgccatc acccggtaaa
    65461 tcccatgacc gacagctatc ttccccgtct ggttgaagcg caatccacag ggcgctgcgg
    65521 cgtcgtttcg gcacatgttt tcgaacaagg cgtggatgcc gttcgtcaag agctggctcg
    65581 cttacagcaa gagggctacc gctacgcggt gcttgatgcg ctgaccgaac accatctgga
    65641 aattcaggga gaagccttgc gcgatgcccc actggtaacg ggcggttctg gtctggcgat
    65701 tggcctggcc cggcagtggg cgcaagaaaa cggtaaccag gctcgcgaag cagggcgtcc
    65761 gctcgctggg cgcggcgtag tgctctccgg ttcatgctct caaatgacca accgccaggt
    65821 ggcacattac cgtcaaattg caccagcccg tgaagttgat gtggcacgct gcctctcaac
    65881 cgaaactctg gccgcttatg cacacgaact ggcagagtgg gttctgggcc aggaaagtgt
    65941 acttgctcca ctggtttttg ccaccgccag cactgacgca ttggcagcaa ttcaacagca
    66001 atacggtgca caaaaagcca gtcaggcagt agaaactctg ttttctcaac tagcggcgcg
    66061 gttagcagcg gaaggcgtga cacgctttat tgtcgcaggc ggtgagacct ccggcgtagt
    66121 cacacagagc ctgggcataa aagggtttca tattggccca accatttccc ccggcgtgcc
    66181 gtgggtaaac gcactggata agcctgtctc actcgccctt aaatctggca acttcggtga
    66241 tgaagccttt ttttcacgag cccaaagaga gtttttatca tgagcgattt cgcaaaagta
    66301 gagcagtctt tgcgagagga gatgacgcgg attgccagtt cattctttca gcgcggctat
    66361 gcaaccggtt cggctggcaa tctgtcgctg cttttacctg acgggaattt actggcgaca
    66421 ccgacaggtt catgcctggg caatctcgat ccgcagcggc tttccaaagt cgccgcggat
    66481 ggcgaatggt taagtggtga caaaccctcg aaagaggtgc tctttcatct ggcgctgtat
    66541 cgcaacaatc cgcgctgtaa agcggtggtg catttgcaca gcacatggtc gacggcgctt
    66601 tcctgcctgc aagggctgga cagcagcaac gttattcgtc cgttcacacc atacgtggtg
    66661 atgcggatgg gaaatgtccc gctggtgcct tattaccgac cgggcgataa acgcatcgca
    66721 caggatctgg cggaactggc agcagacaat caggcttttt tactggcaaa tcatggccca
    66781 gtggtttgcg gtgaaagcct gcaagaagcc gccaacaata tggaagagct ggaggaaacg
    66841 gcaaagctga tttttattct cggtgaccgc ccgatccgtt atctgaccgc aggtgaaatt
    66901 gcggaattaa ggagttaatg caatgcctcg ttttgcagct aatttatcca tgatgttcac
    66961 cgaagtgcct tttattgaac gcttcgccgc agcgcgaaaa gccgggttcg atgctgtgga
    67021 atttctgttt ccctatgact actccaccct gcaaatccaa aagcaactgg agcaaaacca
    67081 tctgacactg gcgctgttca ataccgctcc cggagatatt aatgcggggg aatggggatt
    67141 atccgccctt cccggacgtg agcacgaagc acacgcagac atcgacctgg cactggaata
    67201 tgcattggcg ctcaattgtg aacaagtcca tgtgatggca ggcgtcgtgc ccgctggcga
    67261 agacgctgaa cggtaccggg cagtatttat cgataatctc cgctacgctg ccgaccgctt
    67321 tgcgctacac ggcaagcgaa tattagttga agcactcagc cccgacgtta agcctcatta
    67381 tctcttctcc agccagtatc aggcactggc tattgttgaa gaggttgcgc gagataacgt
    67441 gtttattcaa ctagatactt ttcatgcgca aaaagtagat ggcaacctga cgcatttaat
    67501 ccgcgactac gctgggaaat atgcgcacgt acaaattgcc ggactacctg atcgacatga
    67561 accggacgat ggagaaatca actatccgtg gctgttccgc ctgttcgatg aggtgggata
    67621 tcagggctgg atcggttgtg aatataaacc tcgtggcctc accgaagaag ggcttggctg
    67681 gtttgacgcc tggcgctaat tcgttaaatc cctaattaca acgtacccat acatcccccc
    67741 atctacggat ggggtaggtt aactataact ttcagacagg gtttcccatg tccacaatta
    67801 cattgttatg cattgcgtta gctggcgtaa tcatgctgtt gctgctggtc atcaaggcaa
    67861 aggtacaacc attcgttgct ctgctcctcg tcagcctgtt agtcgcactt gcggcgggta
    67921 taccggcggg cgaagtgggt aaagtgatga tcgccgggat gggcggtgtg cttggttccg
    67981 tcactattat tattggtctg ggcgctatgc tggggcgtat gatcgaacac tctggtggtg
    68041 cagagtcact ggctaattat ttcagtcgca agttaggtga caaacgaact atcgctgcgc
    68101 tgactctggc agcgttcttc ctcggtattc ccgtcttctt tgatgtcggc tttattattc
    68161 ttgcgccaat catttacggt tttgccaagg ttgccaaaat atcgccactc aaatttggcc
    68221 tgcctgtcgc tgggatcatg ctaactgtac atgtggcggt accgccgcat ccaggccctg
    68281 tcgccgcagc ggggttactc cacgcagaca tcggctggct aaccatcatc ggtattgcga
    68341 tttctattcc cgtagggatt gttggctact ttgcagcgaa aataatcaat aagcaccaat
    68401 atgcgatgtc ggtggaagta ctggaacaga tgcaactggc tccggccagt gaggaaggcg
    68461 cgacaaaatt aagcgataaa ataaatccac cgggcgtcgc gctggtcacc tcgctaattg
    68521 ttattcctat cgcgattatc atggcgggta cggtttccgc aacactgatg ccgccttcgc
    68581 atcccctgct tggtacgcta cagctgatcg gctcaccaat ggtagctcta atgattgcgc
    68641 tggtgctggc attctggtta ttggctttac gtcgcggctg gagcttacaa cacaccagcg
    68701 acattatggg ctcagccctt cccactgcgg cggtagtgat tttggttact ggtgctggag
    68761 gggtatttgg caaagtgctg gtggaatcgg gcgtcggcaa agcccttgcc aacatgctac
    68821 aaatgattga cctgcctctg ttaccagccg catttattat ttcactggcg ctgcgtgcat
    68881 cgcaggggtc agcaaccgta gcaatcctga caaccggcgg gttactctca gaagcggtga
    68941 tgggattgaa tccgattcag tgcgtattgg tgacgctggc agcctgcttt ggtgggcttg
    69001 gggcgtcaca tattaatgac tcagggttct ggattgtgac caaatatctg gggttgtcgg
    69061 tagcagacgg tctgaaaacc tggacagtgt taacgaccat tctcggtttt accggattct
    69121 taattacctg gtgcgtatgg gcggtaattt gaccttaata aaaaggtccg atgggcatcg
    69181 gaccttttat tgtgcacaga aaaggccagc ctcgcttgag actggccttt ctgacagatg
    69241 cttacttact cgcggaacag cgcttcgata ttcagcccct gcgtttgcag gatttcacgc
    69301 aaacggcgca ggccttcaac ctgaatctgg cgaacacgtt cacgggtgag gccaatttca
    69361 cgacctacat cttccagtgt tgccgcttcg taccccagca aaccgaatcg acgtgccagt
    69421 acttcacgct gtttggcgtt cagctcgaac agccatttga cgatgctctg cttcatatcg
    69481 tcatcttgcg tggtatcttc cggaccgttc tctttttcat cggccaggat gtccagcaac
    69541 gctttttcgg aatcaccacc cagcggggtg tctaccgagg taatgcgctc gttaagacga
    69601 agcatacggc tgacgtcatc aactggctta tccagttgct ctgcgatctc ttccgcactt
    69661 ggttcatggt ccagcttatg ggacaactca cgtgcggttc gcaggtaaac gttcagctcc
    69721 tttacgatgt gaatcggcaa acgaatagta cgggtttggt tcataatcgc ccgttcaatc
    69781 gtctggcgaa tccaccaggt tgcgtatgtt gagaagcgga aaccacgttc cgggtcaaac
    69841 ttctctaccg cgcggatcag ccccaggttg ccctcttcga taaggtccag caacgccaga
    69901 ccacgattgc cataacggcg ggcaattttt accaccagac gcaagttact ctcgatcatc
    69961 cggcggcgag aggcgacatc tccacgcagt gcgcgacgcg caaaataaac ttcttcttcg
    70021 gccgttaaca gtggtgaata accaatctca ccaaggtaaa gctgagtcgc gtccaacaca
    70081 cgctgtgtgg ctccctgcga taacagttcc tcttcggcca aatcgttatc actgggttcc
    70141 tcttctacta aggccttttc gtcaaaaacc tcaactccgt tctcatcaaa ttccgcatct
    70201 tcatttaaat catgaacttt cagcgtattc tgactcataa ggtggctcct acccgtgatc
    70261 ccttgacgga acattcaagc aaaagcctgg ttccgccgat ttatcgctgc ggcaaataac
    70321 gcagcgggtt tacggatttc cccttgtaac gaatttcaaa atgcaagcgt gttgaactgg
    70381 ttccggtgct acccatggtt gctatttttt gccccgcctt cacttcttgt tgttcccgga
    70441 ccagcattgt gtcgttatgg gcgtaggcac tcaggtaatc atcattatgt ttgatgataa
    70501 tcagattacc gtagccgcgc agcgcgttac cggcataaac aacgcggcca tctgcggtcg
    70561 cgataattgc ctgtcctttg ctgcctgcga tatcaatccc cttgttgccc ccctcagaag
    70621 cgccaaaggt ttcgatcact ttgccctcag tcggccagcg ccaggtggag ataggcgtac
    70681 tggttgatgt actgctgaca gtcggctcgg ttgtgcttgc tgttggtacc gttacaggcg
    70741 ctgtgactgt ggtcgcagtt ggcttgttgt tcggcaacat tttgttagca ctctgttcac
    70801 ccgaagactc agaatacgta attgtcggtt gcgacgcaac agcaacggtg gaattttgtg
    70861 caggcttgat cacaactcct tgctctgctg cgtcggcctg ggtaatggca tttccgccag
    70921 tgattggcgt accggaagca ttacccacct gcaaggtctg accaacgttc agcgcgtatg
    70981 gtgcctgaat attgttgcgc tgagcaaggt cacggaaatc gttgccagta atccaggcga
    71041 tatagaaaag tgtgtcgcct tttttcacgg tataggtact gccgctataa ctgcctttcg
    71101 gaatgttccc atactgacgg ttatagacga tgcgtccgtt ttccatctgt accggctgct
    71161 gagctactgg ctgcactggc tggatttgcg gttgttgagt agcctgaatt tgtggctgct
    71221 gtaccggctg aatttgcggt tgctgggctg tagacgtcgt ccccattttc ggcggcggcg
    71281 taatcaacat accagaatta gtatttgcag gcgcattgcc attaacggag ctgaccggtg
    71341 ccggtggatt tgaagtgtca gaacagcctg ccagccatag cgaaaccagt gacaaagcag
    71401 caatgcggcg aacggtgaat tttgggcttc ccgcgctcat ttatccccca ggaaaaattg
    71461 gttaataacc agtgacataa ttaccgtgca aggcacccta ctgaacactg gaaaagatgt
    71521 tcacgatacg ctgacctgcg gcaaaataac caggaaaaat ccaggtattt cctcacgttt
    71581 taagccagct cacccttcac taaagggaca aagcgcacgg cctccacggt atcgataata
    71641 aattcgcctc cccgacgacg cacccgtttc aaatactggt gctcctcccc tacgggtaag
    71701 acgagaatcc cgccttcgtc cagctgcgtc attagcgcag ttggaatttc cggcggtgcc
    71761 gccgtaacaa tgatagcgtc aaacggcgca cgtgcctgcc aaccttgcca tccatcgcca
    71821 tgacgggttg aaacattatg taaatcaaga tttttcaggc ggcgacgtgc ctgccactgc
    71881 aagcctttaa tccgttcaac cgagcaaaca tgctggacaa gatgcgctag gattgccgtt
    71941 tgatatcccg aaccagtgcc aatttccagc acccgcgact gcggcgtcag ctcgagtaat
    72001 tcggtcattc gcgccaccat atacggctgc gaaattgtct gcccctgacc tatcggcaag
    72061 gcgatattgt cccaggcttt ttgttcaaac gcttcatcaa cgaatttttc acgcggcacg
    72121 gcggcaagtg cattcagcac ctgctcatcc tgaatacctt gcgcacgtaa ttgatccaga
    72181 agtgcttgta cgcgtctgct taccattgcg tgccaactcc cacgctgttt aaccagtctg
    72241 aaaccacatc ttgcgcgcta tgcgcagtta aatccacatg cagcggcgtg atggagacat
    72301 agccctcatc taccgcagca aaatcggtcc ccggaccggc atcacattta ccgcccggcg
    72361 ggccaatcca gtacagcgta ttgccgcgcg gatcttgctg cgggatcacc tgatctgccg
    72421 gatgtcgtgt accgcagcgc gtcacgcgaa tacctttgat ttgatccaag ggtaaatccg
    72481 gaacgttaat attaagaata cgtccggtgc gcagcggctc tttacacagt gcgcgcaaaa
    72541 ttgaacaggt taccgccgcg gcagtgtcgt aatgtttatg cccgtcaagc gagacggcca
    72601 gcgccggaaa acctaaatga cggccttcca tcgcggcggc taccgtaccg gaataaataa
    72661 cgtcatcccc cagattcggc ccggcgttaa tgccggacac aacaatgtcc gggcgcggac
    72721 gcatcagagc attcacgcca agatagacgc aatcggtcgg ggttcccatt tgcacagcaa
    72781 tatcaccatt ttcaaaggta aacgtgcgca gggaggattc cagtgtcaga gaatttgaag
    72841 cgccgctgcg gttacgatcg ggggcgacca cctgaacgtc agcaaactca cgcaaggctt
    72901 tcgccagcgt ttgtataccg ggtgcatgta ccccgtcatc attactcagc aatatgcgca
    72961 taatcacctg ttgtgttgat aagttccctg acaacgctgg ttgcaaaact acccgccgga
    73021 agccagaaac ggatctctac ggtgacgtca tcccaccaat tccagcttaa ttgttgcgga
    73081 tacagcaaca tcgctctgcg cgcggcttca actttttcgc gcaccagtaa agcttgtaat
    73141 tcagtttctg cggcgacagc tgccttttcg aatgccagcg cttcacgctg agttccccat
    73201 tcgccactac ccggcaatgc ggcggttatc atcagctctt tatcgttgac gcgacgctgt
    73261 aattccgcca gttcttcggt ggttgcgaca aaccagctac cacgtccggc taattgtagc
    73321 gcatcgccgt caacaacttg attaacgtct gcttttttga ggcgctcagc aacaatctga
    73381 ttaaacaacg cactgcgggc tgccgacaac caaaaactcc gtttattgcg atcgcgcacc
    73441 ggagtattgg tttgcgccca gcgctgcgcc ccctgcaagt tgctaccgcc aatcccaaaa
    73501 cgttgggcac cgaagtagtt cggtacacct tttacgcaaa tatcgatcag acgttgttca
    73561 acgtcatcgc gattgctcac ttcgcgcaga accagggtaa aggcgttacc tttcagcgcg
    73621 cctaaacgca gcttgcgctt gtgccgcgca tactccagca cctggcagcc ttccagttga
    73681 aaggcgctca gatcgggcat ttccttgcct ggcacgcgag cgcataacca ctgttccgta
    73741 acagcatgtt tgtctttttg cccagcgaag ctgacttcac gggcatgaat tttcaggaat
    73801 ttcgccagtg catccgccac aaaacgggta ttgcagccgt ttttgaggat tctaaccaga
    73861 atatgctcac cttcaccatc aggctcaaag cccaaatctt ccaccaccac aaagtcttcc
    73921 ggattggctt tcagcagccc ggtgccttgc ggtttaccgt ggaggtaagt gagattatca
    73981 aactcaatca ttttgttgcc ttaatgagta gcgccaccgc ttcacaggca atcccttccc
    74041 cacgtccggt aaatccaagt ttttccgtag tagtggcttt cacgttaaca tcatccatat
    74101 ggcagccgag atcttcggca ataaacacgc gcatttgtgg aatgtgcggc aacatcttcg
    74161 gtgcctgagc gataatagtg acatcgacgt tgcccagggt ataacccttc gcctgaatac
    74221 gacgccaggc ttcgcgtagc agctcgcggc tgtcggcacc tttaaatgcc ggatcggtat
    74281 ccgggaacag cttgccgata tcccccagcg ccgccgcgcc aagcaatgca tcggtcaacg
    74341 catggagcgc cacgtcgcca tcagaatgcg ccagcaatcc tttttcgtaa ggaatgcgta
    74401 cgccaccaat gataattggg ccttcaccgc caaaggcatg tacgtcaaaa ccgtgtccaa
    74461 ttcgcattat gtattctcct gatggatggt tcgggtgagg taaaactcgg ccagtgccaa
    74521 atcttccggg cgcgtgactt taatgttatc cgcacggcct tcgaccaact gaggatggaa
    74581 tccgcaatat tccagcgccg aggcttcgtc ggtaatagtc gcgccttcat ttagagcgcg
    74641 cgtcagacag tcatgtaaca gctcacgagg gaaaaattgc ggcgtcagcg cgtgccataa
    74701 gccgttgcga tcaacggtat gagcaatggc atttttgccc ggttcggcac gtttcatcgt
    74761 atcgcgcact ggtgcggcta ggatccctcc cgtgcggctg gtttcgctca acgccaacaa
    74821 tcgcgcgagg tcatcctgat gcagacaagg acgagcggcg tcatgcacca atacccactg
    74881 cgcgtcgcca gcggctttca aacctgccag cacggaatcg gcacgctcat caccgccatc
    74941 tacaacggtg atttgcggat gattcgccag aggaagttgt gcaaaacggc tatcgccagg
    75001 acttatggca atgacgacac gtttcacccg gggatgcgcc agcagcgcat gcaccgagtg
    75061 ttcaagaatg gtttgattac cgattgagag atattgctta ggacattccg tttgcattcg
    75121 acggccaaat ccggccgccg gaaccacggc gcaaacatcc aaatgagtgg ttgccatgtt
    75181 aattcccggg ctgatttatc gattgttttg ccccgcagac tgtgcgcgct tcgacgcgtc
    75241 aggcaccaga cgataaaaag tttcgcccgg cctggtcatg ctgagttcat tacgcgcacg
    75301 ctcttcgagc gcctcctggc cgccattgag atcgtcaatt tcggcaaaaa gttgatcgtt
    75361 tcgcgcttta agtttcgcgt ttgtagcttg ctgtgccgcc acatcatcat tgacgcgggt
    75421 atagtcatgt ataccgttct taccgaacca cagcgaatac tgtagccaga ccagaatagc
    75481 cagcaacagc agcgttagtt tacccatcct gccccctgaa aaacggcatc atcatcccat
    75541 gcatccgaag acgactctac atcctctgtt ggggataccg cgacaacgcg ggcaaatgta
    75601 ccacatttgt ccattgttac gtatacccag ggcgtgcaga acataatctc attattagtt
    75661 acggtttgaa ttatgaacag aggagacaag aaagtacaaa ttagcccagt agccacataa
    75721 acagtgcgcc aaacataatg cctactgtca ccagggtgaa aacaatactg tagcgtagct
    75781 ttccgtccat caatgaatgc agcgcaatcc ccaccactac cgcgacgggc atcagcgcca
    75841 gaaagaaagg ccaggtgtag ataaagaaga acagcgtgtt agagccataa atcaacatcg
    75901 gcatcgccag cgcaaataac caggagataa aaccgaccac ggcaccaggc agtgaccatg
    75961 tggtttcttc atcctcagta aggctgtcgt tatttgttag tgtaatgtta tggctattac
    76021 gcatatttga tcctgttact ttgacgaacc gggcatggaa acccggtggt gtctcaggat
    76081 ctgataatat cgttctgtct caacagatct aataattgct gtaccaaatt tgttactaat
    76141 tgttcaccat tgagatgaat ttctgccgat tcaggcgctt cgtaaacgga atctattccc
    76201 gtaaagttgc gcagttcacc ggcacgcgct ttcttatata agccttttgg atcgcgggct
    76261 tcgcaaatcg ccagcggcgt atcgacaaac acttcgataa agcgcccttc tcctacgcgt
    76321 tcacgaacca tctggcgttc ggcgcggtgt ggcgagataa atgcggtcag caccaccagt
    76381 ccggcttcaa ccatcaaatt cgccacttca ccgacgcgac ggatattctc tttacgatcg
    76441 gcatcgctaa aaccgagatc gctgcataat ccgtggcgaa cattatcgcc atccagcaga
    76501 tacgtactga cgccgagttt atgtaacgcc tcctccagcg ccccggcgac cgttgattta
    76561 ccggacccgg agaggccggt aaaccacagc actacaccac gatgaccgtg gtgtagctcg
    76621 cgttgttgca cagtgaccgg atggctatgc cagacgacgt tttcgtcatg cagcgccatt
    76681 atttatcccc cagcaaatcg cgcgcgcccc agtgcggaaa gtggcgacga accagagcat
    76741 tcaattccag ttcgaatgca ctgaattcag atggcgcagc agttgcctgg ctaactggct
    76801 cgtgcaccat accggcaccc acggtcacat tgctcaggcg atcgataaaa atcagcccac
    76861 ccgtcaccgg attttgttga taacgatcta acaccagcgg ctcgtcaaaa gtgagatcca
    76921 cgaggccgat cccattcagt ggcaggtttt caacttcacg ctgggtaagg ttattaatat
    76981 caacctgata gcgaatgcca tcaacacgcg cgcgcgtctt cttaccggca attttgatgt
    77041 cgtaactctg ccctggagaa agcggctgtt ccgccatcca taccacatcc accgacgcgc
    77101 tctgcaccgc cggtaacgct tcgtctgccg ccagcagcag atcgccacgg ctgatgtcga
    77161 tctcatccgt cagcaccagg gtgatcgctt ctccggcaaa ggcttcttcg cgatcaccat
    77221 caaaagtcac gatccgcgcg acgtttgatt ccacaccaga gggcagcact tttacacgtt
    77281 gcccgacttc cacgcgaccg gatgccagcg ttccggcgta gccacgaaaa tcgagattcg
    77341 ggcgattaac gtactgcacc gggaagcgca ttggctgagc atccaccact ctctggatct
    77401 ccacggtttc cagcacttcg agcagtgtcg gaccgctgta ccacggcata ctttcacttt
    77461 gcgatgccac gttgtcgcct tccagtgcag agagcggcac aaagcggata tccagattac
    77521 ccggcagctg cccggcaaag gtcagataat cttcacgaat acgggtgaac gtctcttcac
    77581 tgtaatccac cagatccatt ttgttgatcg ccacgaccag atgtttgatc cccaacagtg
    77641 tggagataaa actgtgacga cgggtttgat cgagcacgcc tttacgggca tcgatcagta
    77701 agatcgccag ttcacatgtc gatgcgccag tcgccatatt gcgagtgtac tgctcgtgcc
    77761 ctggggtgtc ggcgataata aatttacgct tctcggtaga gaaatagcgg taggccacat
    77821 caatagtgat gccctgttcg cgctcagctt gcaggccgtc caccagcaga gccagatcca
    77881 gcttttcgcc ctgggtaccg tgacgcttac tgtcgttatg cagcgatgag agctgatctt
    77941 catagatttg gcgagtatcg tgcagcaggc gaccaatcag agtgcttttg ccgtcatcga
    78001 cgctgccaca ggtcagaaaa cgcagcaggc ttttatgttg ttgcgcaatc atccaggctt
    78061 cgacgccgcc ttcattggcg atttgttgtg caagtgcggt gttcatctta aaaatacccc
    78121 tgacgttttt tcagctccat agatcccgcc tggtcgcggt caatcacgcg gccctgacgt
    78181 tcactggtgg tggaaaccag catctcttcg atgatttccg gcagtgtttg tgcatttgac
    78241 tccaccgcac cggtcagcgg ccagcagccc agcgtacgga aacgcaccat ccgtttttta
    78301 atcacttcgc ccggttgcag gtcgatacgg ttgtcatcaa tcatcatcaa cataccgtcg
    78361 cgttccagaa ccggacgttc cgcagcgaga tacagcggca caatgtcgat attttccagc
    78421 cagatgtatt gccagatatc ctgctcggtc cagttagaga gtgggaagac gcggatgctt
    78481 tcgcctttgt taatttgccc gttgtagttg tgccacagct ccgggcgctg atttttcgga
    78541 tcccagcgat ggaagcggtc acggaaagag tagattcgct ctttagcgcg ggatttctct
    78601 tcgtcacggc gcgcaccacc gaaggcggca tcaaaaccgt atttgttcag cgcctgtttc
    78661 aggccttcag ttttcataat atcggtatgt ttcgcgctgc cgtgcacgaa tggattaatc
    78721 cccatcgcca cgccttccgg gtttttatgc accagcagct cgcagccgta ggctttcgcc
    78781 gtacgatcgc ggaactcata catctcgcgg aatttccagc cggtatcgac atgcagcaac
    78841 gggaaaggca gcgtacctgg ataaaacgcc ttgcgcgcca aatgcagcat gacgctggaa
    78901 tctttaccga tagagtagag catcaccgga tttgagaatt ctgccgccac ctcgcgaata
    78961 atgtggatgc tttccgcctc cagttgccgc aggtgagtaa gtcgtatttg atccataacc
    79021 gttcctttgc aataccgcta ttttcttgcc atcagatgtt tctgactata gggagcgtaa
    79081 gagaacgaat gaaattacca attagaatga gtagttcctt aacggaataa cgatttggca
    79141 aagctaatat caaaaagtgc ttaaggcacc ggatttctgg cgtttaggaa gatttgaaat
    79201 tgttttagcg cagcggcagt ttcatactat ggcggtaaaa aaatttgcat ggtatttaag
    79261 gactcactat gttttccgca ttgcgccacc gtaccgctgc cctggcgctc ggcgtatgct
    79321 ttattctccc cgtacacgcc tcgtcaccta aacctggcga ttttgctaat actcaggcac
    79381 gacatattgc tactttcttt ccgggacgca tgaccggaac tcctgcagaa atgttatctg
    79441 ccgattatat tcgccaacag tttcagcaaa tgggttatcg cagtgatatt cggacattta
    79501 atagtcggta tatttatact gcccgcgata atcgtaagag ctggcataac gtgacgggaa
    79561 gtacggtgat tgccgctcat gaaggcaaag cgccgcagca gatcatcatt atggcgcatc
    79621 tggatactta cgccccgctg agcgatgctg acgccgatgc caatctcggc gggctgacgt
    79681 tacaaggaat ggatgataac gccgcaggtt taggtgtcat gctggaactg gcagaacgcc
    79741 tgaaaaatac gcctaccgag tatggtattc gatttgtggc gaccagcggc gaagaggaag
    79801 gaaaattagg cgctgagaat ttactcaagc ggatgagtga caccgaaaag aaaaatacgc
    79861 tgctggtgat taatctcgat aacttaattg ttggcgataa attgtatttc aacagcggtg
    79921 taaaaacccc tgaagcagta aggaaattaa cgcgcgacag ggcgctggca attgcgcgca
    79981 gtcacggaat agccgcaacg accaatccgg gtttgaataa aaattatccg aaaggcactg
    80041 ggtgttgtaa tgacgcagaa atattcgaca aagcgggcat tgctgtactt tcggtggaag
    80101 cgactaactg gaatcttggg aataaggatg gttatcagca acgcgcaaaa acagccgcat
    80161 tccctgcggg aaatagctgg catgacgtaa gactggataa tcaacagcat attgataagg
    80221 cacttcctgg aagaatagaa cgtcgctgcc gtgacgttat gcggataatg ctacctctgg
    80281 tgaaggagtt ggcgaaggcg tcttgatggg tttgaaaatg ggagctgggt gttctaccgc
    80341 aggggcgggg aattctaagt gatatccatc atcgcatcca gtgcgcccgg tttatccccg
    80401 ctggcgcggg gaactcttac tgcttggtat gcggaatcac accctgaacg gtttatcccc
    80461 gctggcgcgg ggaacaccct gacgcgccgc agtatttatc tgctctggcc ggtttatccc
    80521 cgctggcgcg gggaacacgg gtgcgtgtgg ctgccagtgc cggagaacgg cggtttatcc
    80581 ccgctggcgc ggggaacaca ctaagcatac ataactgttt ttaaacaaat ttattccgca
    80641 tcaacaatct accaactaaa ttaaaacatt tccttatttt taaagaacgg ataacttatt
    80701 gattatcaac aggaagaaaa gaaaccaaac gtaacccatc caaatccacc ggaatacgtc
    80761 tgttttctcc ccaggtctga aattcaaaac ccgactcggt attggtcgcc caggccatca
    80821 ccacatttcc gcaaccagcc agttgggtaa tttgctgcca gatcatctcc cgaatacgtt
    80881 ttgatgtatc accaacatac acaccggcac gcacttccag tagccagatt gcgagccgtc
    80941 cacgtaagcg cggagggaca ttttctgtaa caaccacgac catgctcatc cgccgcgccc
    81001 ccggtgacca ctatcaccca gcgtttcagg ttcagggatg gcaggcggta acatatccgg
    81061 cgcgggttgt ggtggttcaa tttcacctgc agcaaggact tcctcaatta acggtattaa
    81121 tttgcccgtt aacttagtgc tacggaaaat atcgcgacag gctaatctga cttctttatc
    81181 aggttctgcg ggttgcctcg ctgctatttc aaatgccttt ggcacaaccg aatcaaattt
    81241 aatgatatcg gctatgtcat aaacaaatga aagcggtttg ccactatgga taaacccaat
    81301 agcgggcgca tatcccgcgg ctaatactgc cgcttcagat ataccgtaca gacatgatgt
    81361 agcagcactg atgcagcgat tcacaacatc gcctttttcc cagtctttag gatcgtattt
    81421 gcgaccattc catttcacac catattgttt cgccagtaat gcatacgtct ggcgaacgcg
    81481 agatccctca attccacgta actgctccac tgaacggcga gctggcggtg gttcacgaaa
    81541 acgtaattca tacattttgc gcaccacctt caaccgtaga tcttccgtta aagccagctt
    81601 tgcctagtag agtaatttat ctgcccgcgc ccctccgggt tgtccggagg agtaaacgcg
    81661 aacgcccgct tcaccgaccc agaccagcag tgttcccacc gtggcggcca gatggactgc
    81721 cgcgtgggaa actcttgttc ccggttcgag cataatgcag gcgaccgatc ccactggaat
    81781 atgcgtgcgg atcccggttt tgtcgatcag cacgaaagcg ccgtccagta cgtcgatttg
    81841 accgtactgg aggaagatca tggaagtgcg atcttttaat gggatcgggc tcagcggtac
    81901 aaacgtcacg cctctgctcc gggtttgatc agcatcagac cacaaccgaa cgcccggctt
    81961 ttgccatacc cctgactgag acgttgcaga aataacccag gagcggtgac cgtaagcatc
    82021 cccgtataat ccacgctact gaactggatc agttgccggg agttttcccg ccgcagttgc
    82081 tgttgtcggt aggcatcaac cgaagtatcc agcagtgtaa aaccgcttct ctccccctgc
    82141 gctgccagcc agtccagcgc agcctgttgt tgatgcaacc agacatcact tccttccacc
    82201 tgccccctca cctgccgttt cgcctccatc agcaagtcgt ggcgtttgcc cgctttacaa
    82261 attgttggat tcgcccgcag gttgaaacaa agggattgtc cggtacgcaa ctctggcgca
    82321 aatggtcggc attcgatggt gaaaatatca ctttccgccg ggcgttcctg cgacaggaca
    82381 aaaaagc
//
LOCUS       DAKISA010000003        81960 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.003, whole genome shotgun sequence.
ACCESSION   DAKISA010000003 DAKISA010000000
VERSION     DAKISA010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 81960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81960
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.003"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..346)
                     /gene="pocR"
                     /locus_tag="OVW45_000162"
     CDS             complement(<1..346)
                     /gene="pocR"
                     /locus_tag="OVW45_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein PocR"
                     /protein_id="HCV5524708.1"
                     /translation="MISAATLNSELINKIAQDFAQATSLAVVVVNIHGDEISELFNFT
                     PFCQMMRQHPQLSTRCRMSDRCGGLEASKSDQPCIYRCHAGLTDFSIPLVIAGHLVGF
                     VLCGQVRLRNDDV"
     gene            complement(549..1358)
                     /gene="pduF"
                     /locus_tag="OVW45_000163"
     CDS             complement(549..1358)
                     /gene="pduF"
                     /locus_tag="OVW45_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HCV5524709.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKVAGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCLAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNMVRGSAESLQLASIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWLAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKGISE"
     gene            1870..2154
                     /gene="pduA"
                     /locus_tag="OVW45_000164"
     CDS             1870..2154
                     /gene="pduA"
                     /locus_tag="OVW45_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HCV5524710.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAAHNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            2151..2963
                     /gene="pduB"
                     /locus_tag="OVW45_000165"
     CDS             2151..2963
                     /gene="pduB"
                     /locus_tag="OVW45_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
                     /protein_id="HCV5524711.1"
                     /translation="MSSNELVEQIMAQVIARVATPEHEVLPKTTHQIRETAMAEKSCT
                     LTEFVGTAIGDTVGLVIANVDSALLDAMKLEKRYRSIGILGARIGAGPHIMAADEAVK
                     ATNTEVISIELPRDTKGGAGHGSLILLGGNDVSDVKRGIEVALKELDRTFGDVYANEA
                     GHLELQYTARASYALEKAFGAPLGRACGVIVGAPAAVGVLMADTALKSANVDVVAYSS
                     PAQGTSYSNEVILIISGDSGAVRQAVISAREIGKTVLSTLGDTPKNDHPSYI"
     gene            2982..4646
                     /gene="pduC"
                     /locus_tag="OVW45_000166"
     CDS             2982..4646
                     /gene="pduC"
                     /locus_tag="OVW45_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HCV5524712.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPASQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNVKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTDGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGGFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            4656..5324
                     /locus_tag="OVW45_000167"
     CDS             4656..5324
                     /locus_tag="OVW45_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HCV5524713.1"
                     /translation="MEINEKLLRQIIEDVLAEMQPSDKSVSFRAPVSATVPSAPDTGN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            5339..5857
                     /gene="pduE"
                     /locus_tag="OVW45_000168"
     CDS             5339..5857
                     /gene="pduE"
                     /locus_tag="OVW45_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HCV5524714.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPNASILSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTALDMRITPETLRIQAAIARDAGRDRLEM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLEQRYQAKICAAFVREAAE
                     LYVERKKLKGDD"
     gene            5867..7699
                     /locus_tag="OVW45_000169"
     CDS             5867..7699
                     /locus_tag="OVW45_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HCV5524715.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSFVSSALEETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGFTITPQELLTRPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGTNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVSVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQSGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     PGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPAGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLIAWHKESIHGK"
     gene            7689..8039
                     /locus_tag="OVW45_000170"
     CDS             7689..8039
                     /locus_tag="OVW45_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HCV5524716.1"
                     /translation="MESKERAPAIVVMEIGDCITTWDEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWLAARQSPLLVGIACDREELIVHYKNLPASAPLFTLTYQQDNHTRRSIGTNA
                     ARLVKGIPFREFHS"
     gene            8059..8334
                     /gene="pduJ"
                     /locus_tag="OVW45_000171"
     CDS             8059..8334
                     /gene="pduJ"
                     /locus_tag="OVW45_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HCV5524717.1"
                     /translation="MNNTLGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            8358..8780
                     /locus_tag="OVW45_000172"
     CDS             8358..8780
                     /locus_tag="OVW45_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HCV5524718.1"
                     /translation="MKQSLGLLEVSGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATEASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSKTP
                     ATTVTSEIISCNLCLDPACQRQKGEPRSLCLYANKRGE"
     gene            8783..9415
                     /locus_tag="OVW45_000173"
     CDS             8783..9415
                     /locus_tag="OVW45_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HCV5524719.1"
                     /translation="MDKQTLQETVSQILDEMRERPIPLGVSSRHIHLSSADYLRLFPE
                     QPISEKKPLMQPGQYAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IEGDERRLIFENVAVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVP"
     gene            9412..9903
                     /gene="pduM"
                     /locus_tag="OVW45_000174"
     CDS             9412..9903
                     /gene="pduM"
                     /locus_tag="OVW45_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HCV5524720.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQESVHQY
                     ASLQIRYVDLPLLRQLAENETSDRASIQIHEALAWGMHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHHNRLLSYADIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            9907..10182
                     /gene="pduN"
                     /locus_tag="OVW45_000175"
     CDS             9907..10182
                     /gene="pduN"
                     /locus_tag="OVW45_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduN"
                     /protein_id="HCV5524721.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRRVSADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            10194..11201
                     /gene="pduO"
                     /locus_tag="OVW45_000176"
     CDS             10194..11201
                     /gene="pduO"
                     /locus_tag="OVW45_000176"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
                     /protein_id="HCV5524722.1"
                     /translation="MAIYTRTGDSGSTSLFTGQRVSKTHLRVETYGTLDELNATLSLC
                     YCATAIESHRILLEAIQQQIFWFSAELASESEQPSAQQRYIGTEEIAALENAIDSAMN
                     AVPPVHSFILPGRCEAASRMHFARTVARRAERRLVELTTETTVRNVLLHYINRLSDCL
                     YALARVEDNVAHQNLMIQEITKRYHEANHTPALKERTMPLTFQDLHQLIRSAAMRADE
                     LHIPVVISIVDANGTESVTWRMPDALLVSSELAPKKAWTAVAMKTATHKLADTVQPDA
                     PLYGLESHMQGKVVTFGGGFPLWRDGKLLGGLGISGGSVEQDMDIAQSAMAAINVGVN
                     Q"
     gene            11198..12583
                     /gene="pduP"
                     /locus_tag="OVW45_000177"
                     /pseudo
     CDS             11198..12583
                     /gene="pduP"
                     /locus_tag="OVW45_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
     gene            12594..13706
                     /locus_tag="OVW45_000178"
     CDS             12594..13706
                     /locus_tag="OVW45_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HCV5524723.1"
                     /translation="MNTFSLQTRLYSGPGSLKVLSRFSHRHIWIICDAFLARSPLIDT
                     LHQALPDSNRLSIFSEITPAPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGIGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     VVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGMAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGD
                     PRTAKRYARLAKTCHLCPDNANDTASLNALIQHIEQLNTTCTLPTLANALKEKKAEWS
                     IRIPDMVQAALADATLRTNPRAADASAIAELLEELL"
     gene            13703..15046
                     /locus_tag="OVW45_000179"
     CDS             13703..15046
                     /locus_tag="OVW45_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HCV5524724.1"
                     /translation="MNVGLISCDDQTIRERVQAAGVVGAGGAGFPTHIKLQARADTFL
                     VNAAECEPMLKVDQQLMAQQASRLLRGVHYAMKATGASSGIIALKEKYQRAINALTPL
                     LPPDIRLHILPDVYPAGDEVLTIWMATGRRVPPAALPVSVGVVVNNVQTVLNIARAVE
                     QQYPVTRRTLTVNGAVAKPITVTVPIGMSLREVLALAGGATVDAPGFINGGPMMGSLI
                     TSLDTPVSKTTGGLLVLPNSHSLIQRRMQNDRSVLAVAKTVCEQCRLCTDLCPRHLIG
                     HELAPHLLVRAVNYQQLATPQLLLTALTCSECNVCASVACPVGISPMRINRLLKQELR
                     AQNLRYEGALNPADPMANYRLIPVKRLVTRLGLTPWYQDAPLSEQVPQPEKVTLLLRQ
                     HIGASAIACVQKGDRVVHGQCVGQIPHGTLGAPIHASIDGMVSDVTENAITLVRG"
     gene            15049..15603
                     /gene="pduT"
                     /locus_tag="OVW45_000180"
     CDS             15049..15603
                     /gene="pduT"
                     /locus_tag="OVW45_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HCV5524725.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSAASVRAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            15603..15953
                     /gene="pduU"
                     /locus_tag="OVW45_000181"
     CDS             15603..15953
                     /gene="pduU"
                     /locus_tag="OVW45_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HCV5524726.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTIS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            15958..16401
                     /locus_tag="OVW45_000182"
     CDS             15958..16401
                     /locus_tag="OVW45_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HCV5524727.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     NMEQISLVKCWLREAGAHNVLVTSAVNNHGVAELLALLHTEEGCR"
     gene            complement(16502..16831)
                     /locus_tag="OVW45_000183"
     CDS             complement(16502..16831)
                     /locus_tag="OVW45_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HCV5524728.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(17003..18061)
                     /locus_tag="OVW45_000184"
     CDS             complement(17003..18061)
                     /locus_tag="OVW45_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCV5524729.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSFVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(18259..18732)
                     /gene="sbmC"
                     /locus_tag="OVW45_000185"
     CDS             complement(18259..18732)
                     /gene="sbmC"
                     /locus_tag="OVW45_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HCV5524730.1"
                     /translation="MNYEITQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDNKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(18851..20017)
                     /gene="dacD"
                     /locus_tag="OVW45_000186"
     CDS             complement(18851..20017)
                     /gene="dacD"
                     /locus_tag="OVW45_000186"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HCV5524731.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            20226..21653
                     /gene="sbcB"
                     /locus_tag="OVW45_000187"
     CDS             20226..21653
                     /gene="sbcB"
                     /locus_tag="OVW45_000187"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCV5524732.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNATVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADNKEKVALLKALWQYAEEIV"
     gene            complement(21696..21923)
                     /locus_tag="OVW45_000188"
     CDS             complement(21696..21923)
                     /locus_tag="OVW45_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCV5524733.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(21937..22995)
                     /gene="yeeE"
                     /locus_tag="OVW45_000189"
     CDS             complement(21937..22995)
                     /gene="yeeE"
                     /locus_tag="OVW45_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCV5524734.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAEAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCFIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(23174..24532)
                     /gene="plaP"
                     /locus_tag="OVW45_000190"
     CDS             complement(23174..24532)
                     /gene="plaP"
                     /locus_tag="OVW45_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCV5524735.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(24522..24584)
                     /gene="yoeI"
                     /locus_tag="OVW45_000191"
     CDS             complement(24522..24584)
                     /gene="yoeI"
                     /locus_tag="OVW45_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCV5524736.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(24799..25728)
                     /locus_tag="OVW45_000192"
     CDS             complement(24799..25728)
                     /locus_tag="OVW45_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCV5524737.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLVQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(25774..26598)
                     /locus_tag="OVW45_000193"
     CDS             complement(25774..26598)
                     /locus_tag="OVW45_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCV5524738.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(26681..26935)
                     /gene="yoeB"
                     /locus_tag="OVW45_000194"
     CDS             complement(26681..26935)
                     /gene="yoeB"
                     /locus_tag="OVW45_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCV5524739.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(26932..27183)
                     /gene="yefM"
                     /locus_tag="OVW45_000195"
     CDS             complement(26932..27183)
                     /gene="yefM"
                     /locus_tag="OVW45_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCV5524740.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            27466..27516
                     /gene="hisL"
                     /locus_tag="OVW45_000196"
     CDS             27466..27516
                     /gene="hisL"
                     /locus_tag="OVW45_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCV5524741.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    27492..27615
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            27662..28561
                     /gene="hisG"
                     /locus_tag="OVW45_000197"
     CDS             27662..28561
                     /gene="hisG"
                     /locus_tag="OVW45_000197"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCV5524742.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            28567..29871
                     /gene="hisD"
                     /locus_tag="OVW45_000198"
     CDS             28567..29871
                     /gene="hisD"
                     /locus_tag="OVW45_000198"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCV5524743.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVNALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVKDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            29868..30938
                     /gene="hisC"
                     /locus_tag="OVW45_000199"
     CDS             29868..30938
                     /gene="hisC"
                     /locus_tag="OVW45_000199"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCV5524744.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTQDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLTEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVTQIIAER
                     EYLIAALKEIPCVEQVFDSATNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            30938..32005
                     /gene="hisB"
                     /locus_tag="OVW45_000200"
     CDS             30938..32005
                     /gene="hisB"
                     /locus_tag="OVW45_000200"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCV5524745.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKMALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            32005..32595
                     /gene="hisH"
                     /locus_tag="OVW45_000201"
     CDS             32005..32595
                     /gene="hisH"
                     /locus_tag="OVW45_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCV5524746.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            32595..33332
                     /gene="hisA"
                     /locus_tag="OVW45_000202"
     CDS             32595..33332
                     /gene="hisA"
                     /locus_tag="OVW45_000202"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCV5524747.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPERVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            33314..34090
                     /gene="hisF"
                     /locus_tag="OVW45_000203"
     CDS             33314..34090
                     /gene="hisF"
                     /locus_tag="OVW45_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCV5524748.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDGETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            34084..34695
                     /locus_tag="OVW45_000204"
     CDS             34084..34695
                     /locus_tag="OVW45_000204"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCV5524749.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(34792..35769)
                     /gene="wzzB"
                     /locus_tag="OVW45_000205"
     CDS             complement(34792..35769)
                     /gene="wzzB"
                     /locus_tag="OVW45_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCV5524750.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(35915..37081)
                     /gene="ugd"
                     /locus_tag="OVW45_000206"
     CDS             complement(35915..37081)
                     /gene="ugd"
                     /locus_tag="OVW45_000206"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HCV5524751.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLAIFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(37331..38737)
                     /gene="gndA"
                     /locus_tag="OVW45_000207"
     CDS             complement(37331..38737)
                     /gene="gndA"
                     /locus_tag="OVW45_000207"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCV5524752.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(38901..40271)
                     /gene="cpsG"
                     /locus_tag="OVW45_000208"
                     /gene_synonym="manB"
     CDS             complement(38901..40271)
                     /gene="cpsG"
                     /locus_tag="OVW45_000208"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCV5524753.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALARGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEEKTKLI
                     LELLNK"
     gene            complement(40376..41812)
                     /gene="cpsB"
                     /locus_tag="OVW45_000209"
     CDS             complement(40376..41812)
                     /gene="cpsB"
                     /locus_tag="OVW45_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCV5524754.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGAMHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(41815..43038)
                     /gene="wcaI"
                     /locus_tag="OVW45_000210"
     CDS             complement(41815..43038)
                     /gene="wcaI"
                     /locus_tag="OVW45_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCV5524755.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGSKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLDLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(43035..43514)
                     /gene="gmm"
                     /locus_tag="OVW45_000211"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(43035..43514)
                     /gene="gmm"
                     /locus_tag="OVW45_000211"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCV5524756.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELILPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(43517..44482)
                     /gene="fcl"
                     /locus_tag="OVW45_000212"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(43517..44482)
                     /gene="fcl"
                     /locus_tag="OVW45_000212"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCV5524757.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNELYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(44485..45606)
                     /gene="gmd"
                     /locus_tag="OVW45_000213"
     CDS             complement(44485..45606)
                     /gene="gmd"
                     /locus_tag="OVW45_000213"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCV5524758.1"
                     /translation="MSRVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(45632..46180)
                     /gene="wcaF"
                     /locus_tag="OVW45_000214"
     CDS             complement(45632..46180)
                     /gene="wcaF"
                     /locus_tag="OVW45_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCV5524759.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(46196..46942)
                     /gene="wcaE"
                     /locus_tag="OVW45_000215"
     CDS             complement(46196..46942)
                     /gene="wcaE"
                     /locus_tag="OVW45_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HCV5524760.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            complement(46953..48170)
                     /gene="wcaD"
                     /locus_tag="OVW45_000216"
     CDS             complement(46953..48170)
                     /gene="wcaD"
                     /locus_tag="OVW45_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCV5524761.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVIGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(48145..49362)
                     /gene="wcaC"
                     /locus_tag="OVW45_000217"
     CDS             complement(48145..49362)
                     /gene="wcaC"
                     /locus_tag="OVW45_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCV5524762.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVDEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRATYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(49359..49847)
                     /gene="wcaB"
                     /locus_tag="OVW45_000218"
     CDS             complement(49359..49847)
                     /gene="wcaB"
                     /locus_tag="OVW45_000218"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCV5524763.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(49850..50689)
                     /gene="wcaA"
                     /locus_tag="OVW45_000219"
     CDS             complement(49850..50689)
                     /gene="wcaA"
                     /locus_tag="OVW45_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HCV5524764.1"
                     /translation="MKENPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(50782..52944)
                     /gene="wzc"
                     /locus_tag="OVW45_000220"
     CDS             complement(50782..52944)
                     /gene="wzc"
                     /locus_tag="OVW45_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCV5524765.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWERLMGRQNETVKVTTFNRPKEMAGQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVSWASKNYDLVLIDTPPILAVTDAAIVGRHAGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(52947..53390)
                     /gene="wzb"
                     /locus_tag="OVW45_000221"
     CDS             complement(52947..53390)
                     /gene="wzb"
                     /locus_tag="OVW45_000221"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCV5524766.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(53396..54535)
                     /gene="wza"
                     /locus_tag="OVW45_000222"
     CDS             complement(53396..54535)
                     /gene="wza"
                     /locus_tag="OVW45_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCV5524767.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            54844..54993
                     /locus_tag="OVW45_000223"
     CDS             54844..54993
                     /locus_tag="OVW45_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5524768.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            55194..56777
                     /locus_tag="OVW45_000224"
     CDS             55194..56777
                     /locus_tag="OVW45_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCV5524769.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPRQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIVGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(57051..58904)
                     /gene="asmA"
                     /locus_tag="OVW45_000225"
     CDS             complement(57051..58904)
                     /gene="asmA"
                     /locus_tag="OVW45_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCV5524770.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(58926..59507)
                     /gene="dcd"
                     /locus_tag="OVW45_000226"
     CDS             complement(58926..59507)
                     /gene="dcd"
                     /locus_tag="OVW45_000226"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCV5524771.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(59599..60240)
                     /gene="udk"
                     /locus_tag="OVW45_000227"
     CDS             complement(59599..60240)
                     /gene="udk"
                     /locus_tag="OVW45_000227"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCV5524772.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAINLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            60558..63875
                     /locus_tag="OVW45_000228"
     CDS             60558..63875
                     /locus_tag="OVW45_000228"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCV5524773.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLSLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     VFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLQLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(63985..64833)
                     /gene="alkA"
                     /locus_tag="OVW45_000229"
     CDS             complement(63985..64833)
                     /gene="alkA"
                     /locus_tag="OVW45_000229"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HCV5524774.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADNYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAGR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKRRFPGMTPAQIRRYAERWKPWRSYALLHIWYMEG
                     WQPDEA"
     gene            64967..66319
                     /gene="yegD"
                     /locus_tag="OVW45_000230"
     CDS             64967..66319
                     /gene="yegD"
                     /locus_tag="OVW45_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCV5524775.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQEILERAAKRAGFRDVVFQYEPIAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPQIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(66332..68272)
                     /gene="yegI"
                     /locus_tag="OVW45_000231"
     CDS             complement(66332..68272)
                     /gene="yegI"
                     /locus_tag="OVW45_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HCV5524776.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVSALDSLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(68269..69030)
                     /gene="pphC"
                     /locus_tag="OVW45_000232"
     CDS             complement(68269..69030)
                     /gene="pphC"
                     /locus_tag="OVW45_000232"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HCV5524777.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVVWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(69027..69686)
                     /locus_tag="OVW45_000233"
     CDS             complement(69027..69686)
                     /locus_tag="OVW45_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCV5524778.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(69908..69967)
                     /gene="ibsA"
                     /locus_tag="OVW45_000234"
     CDS             complement(69908..69967)
                     /gene="ibsA"
                     /locus_tag="OVW45_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HCV5524779.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(70240..70296)
                     /locus_tag="OVW45_000235"
     CDS             complement(70240..70296)
                     /locus_tag="OVW45_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCV5524780.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(70567..70623)
                     /locus_tag="OVW45_000236"
     CDS             complement(70567..70623)
                     /locus_tag="OVW45_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCV5524781.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            70902..72143
                     /gene="mdtA"
                     /locus_tag="OVW45_000237"
     CDS             70902..72143
                     /gene="mdtA"
                     /locus_tag="OVW45_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HCV5524782.1"
                     /translation="MKGSYKSRWVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQQS
                     PAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALHFQ
                     EGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSR
                     QELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSG
                     DTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTLLS
                     LDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGH
                     FVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQ
                     SATTPEEKATSREYAKKGARS"
     gene            72143..75265
                     /gene="mdtB"
                     /locus_tag="OVW45_000238"
     CDS             72143..75265
                     /gene="mdtB"
                     /locus_tag="OVW45_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HCV5524783.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFTNMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDQGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQEIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            75266..78343
                     /gene="mdtC"
                     /locus_tag="OVW45_000239"
     CDS             75266..78343
                     /gene="mdtC"
                     /locus_tag="OVW45_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCV5524784.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRHPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            78344..79759
                     /locus_tag="OVW45_000240"
     CDS             78344..79759
                     /locus_tag="OVW45_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCV5524785.1"
                     /translation="MTELPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLITLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            79756..81159
                     /gene="baeS"
                     /locus_tag="OVW45_000241"
     CDS             79756..81159
                     /gene="baeS"
                     /locus_tag="OVW45_000241"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCV5524786.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            81156..81878
                     /gene="baeR"
                     /locus_tag="OVW45_000242"
     CDS             81156..81878
                     /gene="baeR"
                     /locus_tag="OVW45_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCV5524787.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
BASE COUNT        19938 a        21650 c        20812 g        19560 t
ORIGIN      
        1 cgacatcatc attacgcaga cgaacttgcc cgcacaatac aaaaccgact aaatgtccgg
       61 cgattaccag tggaatagag aaatcggtta atccggcatg acagcgataa atacaaggct
      121 gatcggattt agaggcttcc aggcccccac aacgatcgct catgcgacat cgagtgctta
      181 gctgagggtg ttgccgcatc atctgacaaa atggagtgaa gttaaacagc tcggaaattt
      241 catcgccgtg aatattgacc acgacaaccg caaggctggt cgcctgcgca aaatcctgcg
      301 cgattttatt aatgagttct gaattaagtg tcgccgcaga aatcatgata aaacctccca
      361 gttaatttat tgttatagca aaacaattgt tttacattta atcttcacat catccctgat
      421 gatttactga cgagaagcac aaaggacaat aacaaaagcc tccgtgaagc gggagacttt
      481 ttaataagtt cggaaagcag atcacaaaca gtaaaaaaat aaatgtttat aactgccaga
      541 gtatttcgtt attcgctaat tcctttaggt gtaacactat tgacaggtaa attctttgca
      601 atgagatagc gataaatcgc cgcaccaaca catgccccaa tgatcggtgc aataatgggt
      661 acaatgaaat aaggaatatt gcgtccacca gtcatcgcaa tttctcccca ccccgcaagc
      721 caggtgaaaa tcttaggtcc aaaatcacgc gctggattca tggcaaaacc ggttaatggt
      781 ccggtagaag caccaataac agcaaccaga ataccaataa gtaaaggtgc aagcggtcct
      841 ttgggtacgc cattaccgtc atcggtcaac gccataatca accccattaa catggacgtt
      901 atgaccactt cgaccaacgc agcttgccat acattaatcg ccgctgccgg ataagtactg
      961 aagatactgg cgagttgtaa actttctgcg ctaccacgca ccatattgtg agcagattca
     1021 aaatcggtaa acagactact gtatagagaa tatgccagaa ctgcaccacc aaaggcaccc
     1081 gctaattgcg ccagacaaaa tggcagcact ttacgggctg ggaaagaggc aaacagccac
     1141 agagcaatgg taactgccgg gttgagatga gcgccggaaa tacctgccgt aaggtaaaca
     1201 gcaagcgaaa tccctaaccc ccaaatgata caaatttccc acagtcccag acttgccccg
     1261 gcaactttca gggcgcacaa acaaccaatg ccaaaaaaga ggaacagtcc ggtgcccaaa
     1321 aactcggcaa cgcattgcgc cttgagtgaa tcattcatag tgacaccttt tttacggagt
     1381 ttttctttat cagccaacta tgcgctgact gttgtatata ccgagcacaa ctataaaaaa
     1441 ttgctctgca gaaaatattg ccaacatgca tgacagactc gtgtaaaaat cgtgctaaca
     1501 taacaaatta ttgtcattac cgaatcatgc aacacagcgt tagtcagtgt tgaaagtaat
     1561 aattcccacc aaacttttac aataaaaaat tgccatcatg acataataaa attgaaacaa
     1621 atatcacagt tccttcttcg aactaaactc aaccaaatat tactcgcgat taaactcact
     1681 agccaacgca attaattcac attgaaatat ccacaaaaca accaataaat taacaacata
     1741 tagttcataa aagcaatttt ataatatttg ttctctatta atttctctca ttagccgatt
     1801 aagtcagcca ttctttttcc tcgctttttt ttatagtcat atcgtcaaaa aaatccaggc
     1861 gaggtcttta tgcaacaaga agcgttagga atggtagaaa ccaaaggctt aactgccgcc
     1921 atagaggccg cagacgccat ggtgaagtcg gcaaatgttt tgctggtcgg ctacgaaaaa
     1981 ataggttccg ggctggtgac cgtcatcgtt cgtggtgatg tcggggcggt aaaagcggca
     2041 acagacgcgg gtgccgcagc tgcgcataac gtcggcgaag taaaagccgt acacgttatt
     2101 ccccgcccac ataccgatgt cgaaaaaatc ttaccaaagg gaattaaccc atgagcagca
     2161 atgaactggt cgaacagatc atggcgcagg tcatcgctcg cgtagcgacg ccagagcatg
     2221 aggttctccc gaaaaccact catcaaatac gagagacggc tatggcagaa aaaagctgca
     2281 cgctaacaga atttgttggc accgctattg gcgacactgt aggtctggtc attgcgaacg
     2341 ttgatagcgc cctgttggac gcgatgaaac ttgaaaaacg ctatcgttcc attggcattc
     2401 tgggtgcgcg tattggcgca gggccacaca ttatggccgc tgatgaagcg gtaaaagcca
     2461 ccaacactga agttattagc attgaacttc ctcgcgatac taaaggcggt gccggacacg
     2521 gctcactgat cctcttaggt ggcaacgatg tttccgacgt gaaacgcggt attgaagtcg
     2581 ccctgaaaga actcgatcgg acctttggcg atgtctatgc caacgaagcc ggacatcttg
     2641 aactgcaata taccgcccgc gccagctatg cactggaaaa agcgtttggt gcaccactgg
     2701 gacgggcttg cggcgttatt gtcggcgctc cggctgctgt gggtgttctg atggccgaca
     2761 ccgcgctgaa gtccgccaat gtggatgttg tggcatacag ctccccagct caaggcacca
     2821 gctacagcaa tgaagtcatt ctgattatct cgggtgactc cggcgcagtc cgccaggcgg
     2881 tcatctccgc acgcgaaatc ggtaaaaccg tgctttcgac tctcggcgat acaccgaaaa
     2941 acgatcaccc ttcctacatc tgaattctct gaggcagatt tatgagatcg aaaagatttg
     3001 aagcactggc aaaacgcccg gtcaaccagg acggtttcgt taaagagtgg attgaagaag
     3061 gctttattgc gatggaaagc ccgaacgatc ctaaaccgtc aatcaaaatt gttaacggcg
     3121 tcgttaccga actggatgga aaacccgcca gccagtttga cctgatcgac cactttatcg
     3181 cccgctacgg catcaatctc gcgcgcgccg aagaagtcat cgcgatggat tcggtaaaac
     3241 ttgccaatat gttgtgcgat ccaaatgtca aacgtagcga tatcgttccc cttaccacag
     3301 caatgacgcc tgcaaaaatc gtcgaagtgg tttcgcagat gaacgtggta gagatgatga
     3361 tggcgatgca gaaaatgcgc gcccgtcgaa caccatcaca acaggctcac gtcaccaacg
     3421 tcaaagataa cccagtacaa attgccgccg acgcagctga aggcgcatgg cgcgggtttg
     3481 atgaacagga aaccaccgtt gccgttgctc gctatgcacc gttcaacgcc atcgccctgc
     3541 tggtcggctc acaggtggga cgtccgggcg tcttgaccca gtgttcactg gaagaagcca
     3601 ccgaactgaa actcggtatg ttaggccaca cctgttacgc cgaaacaatt tctgtctatg
     3661 gtacagaacc tgttttcacc gatggtgatg ataccccgtg gtcaaaaggg tttctcgcgt
     3721 catcctatgc ttctcgtggc ttaaaaatgc gctttacttc cggctccggc tctgaagtac
     3781 agatgggtta cgcggaaggc aaatcaatgc tttacctcga ggctcgttgc atttacatca
     3841 ccaaagccgc aggcgttcag ggtctgcaga atggttcggt aagctgcatc ggtgttccat
     3901 cagctgtgcc atcaggcatc cgcgcagtat tagcggaaaa cttaatttgc tcatcgctgg
     3961 atctggaatg cgcctccagt aatgaccaga cctttaccca ctcggatatg cgccgtaccg
     4021 cacgtttcct gatgcaattt ctgccaggca ccgactttat ctcttccggt ttttccgctg
     4081 tgccgaacta cgacaacatg tttgctggtt caaacgaaga tgctgaagac ttcgatgatt
     4141 acaacgtcat tcagcgtgac ctgaaggttg atggtgggct gcgaccagta cgcgaagaag
     4201 atgttatcgc tatccgcaat aaagccgctc gcgctttaca ggctgtcttt gccggtatgg
     4261 gactaccgcc tattaccgat gaagaagtcg aagcggcaac ctatgcccac ggttcaaaag
     4321 atatgcctga gcgcaatatt gtggaagaca tcaagttcgc ccaggagatt atcaataaaa
     4381 accggaacag cctggaagtg gtgaaagcct tggcgcaagg cggttttacc gatgtcgccc
     4441 aggacatgct caacatgcaa aaagcaaaac tgaccggcga ttatctccat acctcagcca
     4501 tcatcgtcga tgacggacaa gtgctctctg cggtcaatga cgtcaatgat tacgccggac
     4561 cggctacagg ttaccgcctg caaggggaac gctgggaaga gatcaaaaat attcccggtg
     4621 cacttgatcc caacgaaatt gactaagggt gagaaatgga aattaacgaa aaattactgc
     4681 gtcagattat cgaagacgtg ctggcagaaa tgcaacccag cgacaagtcc gtttcatttc
     4741 gcgcacctgt ctctgcaact gtcccttccg cgccagatac cggaaatttt ctgaccgaaa
     4801 ttggcgaagc ccaacaaggc actcagcaag atgaagtcat tattgctgta ggcccggcgt
     4861 tcggcctggc gcaaaccgtc aatatcatcg gtattccgca taaaaacatc ttgcgcgaag
     4921 tgattgccgg tatcgaagaa gaaggcatta aagcccgtgt aattcgctgc tttaaatcgt
     4981 ccgatgtcgc attcgtagca gtcgaaggcg atcgtcttag tggttcaggt attgctatcg
     5041 gtattcagtc gaaaggcaca acggttatcc accagcaggg acttcctccc ttgtctaacc
     5101 tggaactttt cccacaagcg cctttgttga cgctggatac ttatcgtcag attggcaaaa
     5161 acgccgcgcg ttatgcgaag cgcgagtctc cacaaccggt gccgaccctc aacgatcaaa
     5221 tggcgcggcc gaaataccag gccaaatctg ccattttgca tattaaagag acgaagtacg
     5281 tggtcactgg caaaaaaccg caagaactgc gcgtgacgtt ttaacaaagg aaaccttcat
     5341 gaataccgat gcaattgagt cgatggtcag ggacgtgttg aaccgcatga acagtttaca
     5401 ggatgcagct cctgtttctg cggtccccaa cgcatcaata ctgagcgcca aagtgacgga
     5461 ttacccgctg gcaaataaac atccggaatg ggttaaaaca gcgaccaata aaacgctcga
     5521 cgatttcact ctggaaaatg tgctaagcga taacgtcacc gccctggata tgcgcattac
     5581 ccctgaaaca ttgcgtattc aggctgcgat tgccagagat gctggtcgcg atcgtctgga
     5641 gatgaatttt gaacgcgccg ctgagcttac ctcagtacca gatgatcgca tcctcgaaat
     5701 ctacaatgcg ttacgccctt atcgttcaac taagcaagaa cttattgcca ttgctgacga
     5761 tcttgaacag cgttatcagg ccaaaatttg tgctgcattt gtccgtgaag ctgcggaact
     5821 ttatgtcgag cgcaaaaaac tgaaaggcga cgattaagga cgaactatgc gatatatagc
     5881 aggcattgat atcggcaact catcaacaga ggttgcgctg gcaacgctca gtgcaactgg
     5941 agagttgtcg ttcgtgagta gcgccctgga ggaaaccacc ggaatcaaag gtacgttgcg
     6001 taacgtccat ggtattcagg aggcgctcgc acaggcaacg aaaaaagtcg gcatcaatgt
     6061 tagcgatatt tcgcttattc gtatcaacga agcaactccg gtcattggtg atgtggcgat
     6121 ggaaaccatc actgaaacta tcatcaccga atcaaccatg atcggtcaca acccgaaaac
     6181 tccgggcgga gtggggctgg gcgtagggtt caccatcact ccgcaagagt tattaacacg
     6241 cccggcggac acaccttaca tcctggtggt gtcatcggca tttgattttg ccgatatcgc
     6301 gacgatgata aacgcctccg tacgcgccgg atatcagctg actggcgtta ttctgcaacg
     6361 cgacgatggc gtactggtca ataaccgcct ggaaattcct ttacctattg tggatgaagt
     6421 cctctacatc gatcgtattc cgctcggcat gctggcagca attgaagttg ccgttcccgg
     6481 gaaagttatc gaaaccctgt ctaacccgta tgggattgcc accgtattcg ccctcaatgc
     6541 tgaagagact aaaaatattg ttcctgtcgc gcgggcgcta atcggcaacc gttcagcagt
     6601 ggtagtgaaa acgccctctg gcgacgttaa agcccgttcg atacccgccg gaaatatcga
     6661 actgctttct gccgggcgca caacgcgcgt tgatgtagct gctggtgccg atgccattat
     6721 gaaagcggtc ggtgagtgcc caaaactgga aaacgtgacg ggagaaccag gcaccaacat
     6781 cggcggaatg ctggaacatg ttcgccagac catggcggaa ctgaccaaca aacccagcaa
     6841 cgaaattttt attcaggatt tgctggcgat agatacctct gtccccgtca gcgtaaccgg
     6901 tggtctggca ggtgaatttt cactggaaca ggctgtgggt attgcctcta tggtgaaatc
     6961 cgaccgtctg caaatggcga tgatcgccag tgaaattaag caaaaactgc atgttgatgt
     7021 tcaggtcgga ggcgcagaag cagaagccgc cattcagggg gccttgacta cccctggaac
     7081 cacacgtcca ctggcaattc tggacttagg tgcaggctct accgatgcct ccatcatcaa
     7141 tcagagtggg gaaatcgttg ccactcacct tgcgggagcg ggcgacatgg taacgatgat
     7201 tatcgctcgt gagctgggct taaatgatcg ttatctggca gaagaaatca aaaagtaccc
     7261 gttagcaaaa gtagaaagcc tgttccactt acgccatgaa gacggtagcg ttcagttctt
     7321 ccctactccc ctatcccccc atgtatttgc ccgcgtctgt gtagtgaaac ccgacgaact
     7381 ggtgcctatt cccggtgatt tgacgctgga aaaagtacgc gctgttcgtc gcagtgcgaa
     7441 agagcgtgtc ttcgtaacca atgccctgcg cgcgctacgt caggtaagcc ctgccggaaa
     7501 tattcgcgac attccgttcg tcgttctggt gggtggttct tcgctggatt tcgaagtacc
     7561 gcaattggtc accgacgcgc tggcacatta tcgactggtc gccgggcggg ggaatatccg
     7621 tggtagcgaa ggtccgcgca acgcggtagc tactgggcta ctcatcgcct ggcacaagga
     7681 gtcgattcat ggaaagtaaa gagcgtgcac cagccatcgt tgtcatggag atcggtgact
     7741 gtatcactac atgggatgag gtgcttcttg gcattgaaga ggaaggtatt ccctttcgca
     7801 ttcaacacat tccgtcaggt gaagttatcg atagcgcctg gctggctgct cgtcaatctc
     7861 ccctgttggt tggcattgcc tgcgaccggg aagaactgat tgtgcattac aaaaacttac
     7921 cggcatcagc gccactgttt acgctgacgt atcaacaaga taaccatacc cggcgcagca
     7981 tcggcactaa cgctgcccgc ctggtaaaag gaattccctt ccgggaattc cactcataat
     8041 ccacaggaga aacgctgtat gaacaacaca ctaggactgg ttgaaacaaa agggttagtt
     8101 ggcgcaattg aagctgcaga tgccatggtt aaatccgcca acgtgcagct ggttggctat
     8161 gaaaagattg gttcgggtct gattacggtg atggtccgcg gtgatgttgg cgcagtaaag
     8221 gccgccgttg atgcggggag cgctgcggca agcgccgttg gcgaagtgaa atcctgccat
     8281 gtcatcccgc gcccacacag tgatgttgag gctattttac ctaaatcggc ataaccccat
     8341 ttgctaagga gcacagcgtg aagcaatcac tgggattact tgaagtcagt ggtctggctc
     8401 tcgccatcag ttgcgcagac gctatggcaa aagccgcgtc catcacgctg gttggccttg
     8461 agaagaccaa tggctcaggc tggacgctca tcaaaatcac cggtgacgtc gcctcggtta
     8521 atgcggcgat cgcgacggaa gccagtttcg ccggacagcg aaatggcctt gtcgcacaca
     8581 aagtgattgc ccgcccggga gacggcattc tgccccacac aactgaagca ttgaccgtga
     8641 cggttgaaac aagcaaaaca ccagcaacaa ccgtcacctc agagattatc agttgtaatt
     8701 tgtgtcttga cccggcatgt cagcggcaaa aaggtgagcc ccgctcactt tgcctgtacg
     8761 ccaataagcg aggggagtag cagtggataa acaaaccttg caggaaacag tgagccagat
     8821 tctggatgag atgcgcgagc gccctatccc actgggcgtt tccagtcgcc atatacatct
     8881 ttcatcagcg gattatttgc gtctttttcc tgaacaaccg attagcgaga aaaagccgtt
     8941 aatgcaacct ggccaatacg ctgctgaaca ggttgttacg ctggagggac caaaaggcaa
     9001 attgcacaac gttcgcttgt tggggccgct acgcaatgca agccaggtgg aaatttcccg
     9061 gacggacgcc agaacgttag ggatcaccgc cccgctacgc atgtcaggcg atctcagcgg
     9121 cactccgggg attcgtttga taagcccatt tgcggaactg gagatcacat ctggcgtcat
     9181 tgtcgcgcaa cggcatattc atatgtctcc gctggatgcc ctgatttatg gcgttacgca
     9241 tggcgataaa gtggcggttg ccattgaagg tgatgagcga cgactgatat tcgaaaacgt
     9301 cgctgttcgc gtctctccgg atatgcgtct tgaaatgcat attgataccg atgaggccaa
     9361 cgccgcaggc gctgatagcc ctgacgtttt tgccacactg gtgtcagtgc catgaataac
     9421 atgctgatgc aacgtattgt tgacgaagtg gtttttcgcc ttaaacaacg tgcgggaaaa
     9481 acactcgtgc tgactgtttt tcaattgcga gacgccagcg ttcaagagag cgttcatcag
     9541 tatgcgtcat tacaaatccg atatgtcgat ctgccgctgc tgcgacaact tgcggagaac
     9601 gaaacgtcag atcgagcatc tatccagata cacgaagcgt tagcctgggg aatgcacatt
     9661 caattgtcgt tgcagcgtca tttcctgaac gccatcgaat tgaagactct cgcccgtcta
     9721 ccgctaagct ggtgcgacga acaagggcag ccgatctacc ttcatcataa tcgactttta
     9781 agttatgccg atatcgcaca gttaagctcg ggaatactgg tgttacagcg gaaatgttgt
     9841 gtgaccgcac tggctcgtga agcggcaatt acccgaaata ttcagttaat caggcaggag
     9901 taacctatgc atctggctcg agtcactggc gcggtagtat ccacgcaaaa atcaccttct
     9961 ctgatcggga aaaaactgct gctggttcgc cgggtcagtg ctgacggcga actgcctctc
    10021 acgccattaa ccggcgacga agttgccgtt gattcagtcg gcgcaggcgt tggtgagttg
    10081 gtactcctca gcagtggttc cagcgccaga catgtgtttt caggccctaa cgaggccatc
    10141 gacctggcag tggtcggcat tgtcgatacg ctttcgcgct aaggagctaa aagatggcaa
    10201 tttatacccg aacaggtgat tcaggaagta cgtctttatt taccggacag cgcgtcagca
    10261 aaactcatct acgtgtcgaa acctatggca cgctggatga actgaacgcg acactcagtc
    10321 tgtgttattg cgccaccgcc atcgaaagcc atcgcatctt actggaagcc attcaacaac
    10381 agatattttg gtttagcgcc gaactcgcca gtgaaagcga acagccgtca gcgcaacagc
    10441 gttacatcgg tacggaagaa attgccgcgt tggaaaacgc gatcgatagt gcaatgaacg
    10501 ccgtcccccc agttcacagc ttcattctgc ctggtcgatg tgaagccgca agccgcatgc
    10561 atttcgcccg cacggtggct cgacgggctg aaagacggct ggttgaactg acaacggaaa
    10621 ccaccgtccg gaatgttttg ctgcactata tcaaccgact ttccgattgc ctgtatgccc
    10681 tcgcccgcgt ggaagacaac gttgctcatc aaaacctgat gattcaggaa atcacaaaac
    10741 gttatcacga agccaaccac acaccagcat tgaaggaacg cacaatgccg ctcacttttc
    10801 aggatcttca ccagctcatt cgtagtgcgg caatgcgcgc ggatgaactg catattcccg
    10861 ttgtcatcag cattgttgat gccaacggaa cagaaagtgt tacctggcgg atgcctgatg
    10921 ccctgttggt cagcagtgaa ctggcaccga aaaaagcctg gaccgccgtg gcgatgaaaa
    10981 cggcaaccca caagcttgct gataccgtgc aacccgacgc gccgctttac gggctcgaaa
    11041 gccatatgca aggaaaagtc gtcacttttg gtggcggctt ccccctctgg cgtgacggaa
    11101 aattgcttgg cgggcttggc atcagcggcg gtagcgttga acaagacatg gatattgccc
    11161 aaagcgcaat ggcggcaatt aacgtgggag taaaccaatg aataactctg aattagaaac
    11221 actgatccgc aatattctgc gtgagcaact tgtccccgct acaccacgaa cgcaaagaaa
    11281 cgctattttc cagactgtcg atgaagcggt ctgcgcagca cactaggcct ttttacgctt
    11341 tcaacagtgc ccgcttaaaa cccgtagcgc cattatcagc gccattcgcg aagaattaat
    11401 gccccgcctt accgaactgg cagaagaaag cgccaaagaa acggggatgg gaaataaaga
    11461 agataagtac ctaaaaaaca aagcggcact cgataacacg ccaggaattg aagatttaac
    11521 caccacagca ctaaccggtg atggtggcat ggttttgttt gagtactctc cgtttggggt
    11581 gattggctcg gtcacgccaa gcaccaaccc cacggaaacc atcattaaca acagcatcag
    11641 catgctggca gcaggtaata gcgtctactt cagtccacac ccgggtgcca aaaacgtttc
    11701 gttaaaactg attaccacaa tcgaagacat cgcgtttcgc tgtagtggca ttcataactt
    11761 gatcgttacc gtatctgaac cgacctttgc tgccaccgaa cagatgatgg cgcaccctaa
    11821 aatcagcgtt ctggcgatta ccggcggtcc ggggatcgtc gccatgggaa tgaaaagtgg
    11881 caagaaagtg attggtgccg gagcgggtaa ccctccgtgc atcgtcgatg aaacggcgga
    11941 tattgtgaaa gccgccgagg acatcatcaa cggcgcttca tttgattaca acctgccgtg
    12001 tattgctgaa aaaagcctga ttgtcgtggc atcagtggca gattatttaa ttcagcagat
    12061 gcaaagtttt ggcgcacttc ttcttaacta tgagcaaact gagaaattgc gtgcaatttg
    12121 cctgccagac ggtagcgcca acaaaaaact ggttgggaaa agcccatcag ccttgctgga
    12181 agcggcaggc ctccctttgc ccgcaaaagc gccgcgccta ctgattgctg ttgtcgacgc
    12241 cgatgatagt tgggttactt gtgagcaact aatgccaatg ctgcccattg tgaaggtcaa
    12301 ggattttgac agcgctctaa ccctggcgct gaaagtggaa gacggcttgc accacacggc
    12361 aattatgcac tctcagaacg tctcacgact caaccttgcc gcgcgcgtta tgcaaacctc
    12421 aatatttgtc aaaaacggcc cctcctgggc gggtattggc gtcggcggcg aaggctttac
    12481 gacctttacc attgcaacac cgactggaga gggtacaacg tcagcacgga cttttgcccg
    12541 ctcacgacgc tgcgtgttaa ccagcggctt ttcaattcgc tagagaaacg gaaatgaata
    12601 ctttttcact gcagacgcgt ttatacagcg gaccaggaag tctcaaggta cttagccgtt
    12661 tctctcatag acacatctgg attatttgcg atgcctttct ggcacgctca ccacttattg
    12721 acacgttgca tcaggcgctg cccgacagta atcgtctcag tatttttagc gaaataacgc
    12781 ccgccccaac tatccagacc gtggtgaaag gcattgcgca aatgcagact ttacgcccgg
    12841 atgtagttat cggttttggc ggcggttctg ccctggacgc cgcaaaagca attgtctggt
    12901 tcggtcgcca gtgtggcatt gaaattgaaa cctgcgtagc cattcctacc accagcggta
    12961 tcggttccga agtgaccagt gcttgtgtca tcagcgatcc tgagaaaggg attaaatatc
    13021 cccttttcga taatgcactt tatccggata tcgcgatcct tgatccatct ttggtcgtca
    13081 gcgtgccacc ggcgataacg gccaataccg ggatggatgt tttaacccat gcgcttgaag
    13141 cttatgtttc tcctcgcgcc agcgacttca cggatgcact ggtcgaaaaa gccgtgcaaa
    13201 ttgtttttca atacctgccc actgcagtga aaaaaggcga ttgtcttgcc acccgtggca
    13261 aaatgcataa tgcatcgacg cttgcaggaa tggcctttag ccaggccgga ctcggtttaa
    13321 accatgcaat tgctcatcaa ctcggcggac agtttcatct gccgcatgga ctggcgaatg
    13381 cgctcctgct cacggcagtt atccgcttta atgctggcga tccgcgcaca gccaaacgct
    13441 atgcccggct ggcgaaaacg tgccatttat gcccggacaa cgccaatgac accgccagcc
    13501 tgaatgcact tattcaacat attgagcagc ttaatacaac ctgtacgctg ccgactcttg
    13561 ccaatgcgct gaaagaaaaa aaagcagaat ggtcaatacg cataccagat atggttcagg
    13621 cggcgctggc ggatgcaacg ttgcgtacca acccgcgtgc agctgatgcc tccgcgattg
    13681 cagaactgct cgaggagttg ttatgaacgt cggattaatc tcctgcgacg accagaccat
    13741 ccgcgagcga gtacaagcag caggtgtcgt gggtgctggc ggcgcaggtt ttccaaccca
    13801 tattaagcta caagcgcgtg ctgacacttt cctggtgaat gcggcggaat gcgaacccat
    13861 gttgaaagta gatcaacagt taatggcgca gcaagccagc cgcctgcttc gcggtgttca
    13921 ctatgcgatg aaagccaccg gggccagtag cggaatcatc gcgctgaaag aaaaatatca
    13981 acgggcaata aacgcactca caccactgtt accaccggat atccgcctgc atattttgcc
    14041 cgacgtttac cccgcaggag acgaagtgct gaccatctgg atggcaactg gtcgccgcgt
    14101 tccacccgct gcattacccg ttagcgtcgg tgtggtggtc aataatgttc aaaccgtact
    14161 taatattgcc agagccgtcg agcaacagta tccagtgacg cgccgcacac tcaccgttaa
    14221 tggtgcagtg gcgaagccaa taacggtaac cgtccccatc ggcatgtctt tacgtgaagt
    14281 cctggcgctg gcgggcggcg caacagtgga tgctcctggt tttatcaatg gcgggccgat
    14341 gatgggaagt ttgatcacat cgctcgacac accagtcagc aaaaccactg gcggcttgct
    14401 ggttttaccc aactctcatt cactgatcca acgtcgaatg cagaatgacc gcagcgtact
    14461 tgccgtcgcg aaaactgttt gtgagcaatg ccgcttgtgt accgatctct gcccacgtca
    14521 tttaatcggt cacgaactgg caccccattt attagtacgt gcggttaact accaacagct
    14581 cgccacgccg caactgttac tgacagcgct cacctgttca gagtgcaacg tctgcgccag
    14641 tgtcgcctgt ccggtaggaa tttcaccaat gcgcataaac cgcctgctta agcaggaatt
    14701 acgcgcacaa aatctccgct acgaaggtgc cttaaatcct gcagatccca tggcaaatta
    14761 tcgactgatt cccgtaaaac ggctggtaac cagacttggc ttaaccccgt ggtatcagga
    14821 tgcgccattg tctgaacagg tgcctcagcc cgaaaaagtg acgttattgt tacgccagca
    14881 catcggtgcc agcgcaattg cgtgtgtaca aaaaggcgat cgcgtcgttc atgggcaatg
    14941 tgtaggtcaa attccacacg gcacattagg tgcccccatt catgccagta ttgatggcat
    15001 ggtcagcgat gtgacagaaa acgcgattac gcttgtgaga ggttaaccat gtcgaaagcg
    15061 gtaggtattt tagaacttag cagtatcgcg aaagggatgg aaaccggcga cgccatgctg
    15121 aaaagcgcca acgttgagtt actggtcagc aaaacgctat gccccggaaa atttctgctg
    15181 atgctcggtg gcgatgtggg cgcggtgcaa caggccatca acagcggagc gtcccttgct
    15241 ggtgaaatgc tggtcgacag tcttgtcctg ccgaatattc atcccagcct cctttctgca
    15301 atcagcggac ttaaccatgt tgaacagcac cgggctgtcg gcgtagtcga aacctggagt
    15361 gtcgccgcct gcattgacgc tgccgattgt gctgtcaaag ctgcaaatgt cattcttgtt
    15421 cgcgtacata tggcttttgg cattggcggt aaatgctaca tggtggttgc tggcgatatt
    15481 tcagatgtta ataacgcggt ctctgccgcc agcgtgcgcg cgggggaaaa aggtttgttg
    15541 gtctatcggt cggtgattgc ccgtccacat gaagccatgt ggcgtcagat ggtggaggga
    15601 taatgacaac tcagcacaca actgaacgga tgattcagga atatgtccca ggtaagcagg
    15661 tcacgctggc acatgtcatc gccaatccag ggaaggatct gtttaagaaa ttgggtttac
    15721 cagacaccat ttccgctatt ggtattctga caattacccc cagcgaagca tctatcatcg
    15781 catgcgatat cgccacaaaa tcaggcgctg tagagatcgg ttttcttgat cgttttaccg
    15841 gtgccgtagt gctgactggt gatgtttctg cgatcgaata tgcacttagc caggttaccc
    15901 gtacgctggg tgaaatgatg cgctttaccg cctgccccat tacgcggacc tgacgttatg
    15961 caacgtatga tgctcatcgg ccccagccag tgcggaaaaa cgtcactcac gcaatgtctg
    16021 cgtggcgaaa atattcatta tcagaaaacg caggcaatcg tctggtcccc gaccaccatt
    16081 gatacaccag gcgaatacct cgaaaaccgc agcctctaca gtgcactgtt ggtcagtgcc
    16141 tgcgaagcgg acattatcgc gctggtgctt aatgcggacg caccgtggtc acctttttcg
    16201 ccaggcttta cctcgtcaat gaatcgcccg gtcattggag tagtaaccaa agcagattta
    16261 gccaacatgg aacaaatatc tttggtgaaa tgttggttac gggaagctgg agcgcacaac
    16321 gtgttagtaa ccagtgcggt taataaccat ggagttgcag aacttttagc cttgctgcat
    16381 acagaagaag gctgtcgcta atttcgacag gttatcagat gggaatgatg acggtgatac
    16441 acagaaaaga atcaatgagc aatgagggtt gccgggcaac cctcattgaa taaaacagga
    16501 attacttcgc ttcgccgttt ttcatttcgc ccatagcttt cagctttttg gagatatcgc
    16561 ggcgttcttt ggagagctcg gcatttttga tgatgtaatc gtcaacgcga tcttcatagt
    16621 cacctttcat gctggcgatg atgccctgga ttgcttcaac gctcatccca ggcttgatgt
    16681 aatcgctcag gttgtccagc agcaggacgc gcttctggtt gtcacggatc tttttctcaa
    16741 cgtcctgaat ttcacgttgc agtttgttct tacgacggaa cagacgaaca aattccagta
    16801 cgtcctggaa tgaaggcttg gtagtttcca tttttatacc cctgataatg tgagagtcgg
    16861 attcgtttaa gcaaccgcta ttcgttaggg ccaacattac tcatagctgc cgcagatgac
    16921 aatgctttta tcccttttca ctatcatacc ctttatcctt gctgaatcga agcagcagca
    16981 agatgattct gaagttcagg aattatttgc gcaaggcccg gcaaatcaga ttcgatagca
    17041 actcaagctg atgcgccgtt tccatgttca gccacacata accatagatt ggtgtttcca
    17101 ctaccttcag gtcatggtgg ttattgagca actgacgcag ctcttccaca gcatcgttca
    17161 atttttccgt attggcaaaa accggctgcg gattaccttc gtacagcgca tgaacaaagc
    17221 tcagcagtat ttgctgcatc atgtgctggg tatcacgaag tttttgcgcg ttcaataaca
    17281 cgaaatggct ggggcgcgtg gcccagtacg cattgatttg caactccagc atacaaacca
    17341 gattgcggtt aatggtctgg ataccttcat atatcgattt tggaatacgg gtttctttgc
    17401 tggcgggcgc aatcagccca cgcattttca cggcatcggt caggagtttt tgtagatggc
    17461 tttccagacg tgggcgttcg agtaagttcg gtgagaatgc agattgatag acccgattat
    17521 actcggtcag acttttcgcc agttgaatgc gccagtggat gaacgcccgt tgtggccaga
    17581 taccggtaaa caacattgcc agtaaagagc cgaggatcac atcgccgctt cgccataacg
    17641 ccgtatcaat ttcacctgtc ggggaaccaa caacaattgc cagcgtcacc ccaatcaata
    17701 atccttgata cggtttcttg cccagcgcca gccaaccgca aagaaacatc gccgccgcgc
    17761 accagactaa catcagcggt aacgaaatta actccagttg cagagcgata agacctaaaa
    17821 tcgaacccaa caccgtaccg ccaatacgtt cgaaggcacg agggacaacg ttaccccaga
    17881 acgagattgg ccccataatc accaccatgg tgaccagcgg ccaggtgctt tccgggatag
    17941 taaacaggcg gataatgaga aaagtgagca ggaatgccag cgcgacccga gtaccatgca
    18001 ctatgcggta atggcggtat acccggattt caaacgggct taatgactta tcggcacgca
    18061 cgcagaaact ccgtaatgaa gattaaaagc gctcataact catctgtcgg ccagaaggcg
    18121 cgcctctgaa ttatccgacg gacaaaccgg gaacagtgta atttcccggc aggagcgaaa
    18181 aaattgaaag gcgcatctta ctcttttcgc tttcaaaaaa agagtggtca tcgcgttaac
    18241 acaccgccct gagatgaatt agtgatgttt tggctgcacc gcaacataca tttcgatatc
    18301 ccagtaccca tcttccgcgc cattgttcaa ataaacctcg aagcagggct ttggtaacat
    18361 ttcataagca ctgtcctgca agaggctatt aaagaactga taccagggtt tagcaaaatc
    18421 atcaccgact acacgagcca ccgccaccgc atactgacca cctgaaattt ctgtcagaat
    18481 gacgccctca ctgttttcgg gaagcgtaaa gttattcggt accgtcacaa cggtgtcgca
    18541 gcgtaatttt tcggcgggtg tttcatctgg attgtcgtaa tagacagcaa cccactcctt
    18601 cggcacaata tttttgttat ctacccacat catcaactgc tcaaagcctt tctttaccgt
    18661 ctgttcccac gggccaacga gatggaaacc tgcaacggta cgtttctctt cctgcgtaat
    18721 ctcgtagttc atgacgcctc cattaacact gtttttatat acagcatagt tgcaatatta
    18781 aaaccacaag gaatgagtgt tgattatgcg agcagactcg cactcctgcc agtctgctgc
    18841 aaaagaaagg tcaggcctta tggtggaaat aatcactcag gcgggaaaac atgccgcctt
    18901 ccccgacaga ttccagggta accagcggcc agtgcgccac ctgtttatca cggtcgtaaa
    18961 gttcaatttc ccctacccgc tgatgggcgc taattggcgc ggtgagctct ttaccatcaa
    19021 gggtatattt ggctttgata tgtggaattt cggctttcgg tagcaccatc cagaactctt
    19081 gttccgttcc taggtcgata ttttctttat cgccatacca gatgcgttcc gttccgacct
    19141 ttttcccacg gtgcaaaatt tgcaccgtag taaagttttg ttgcccccaa cgcagtaatt
    19201 ttcttgcctc ttcctcacga ccttttgcac tgtcagcccc cattaccact gcaatgagac
    19261 gacgctgccc atctacagcc gaagcaatga gattaaaccc ggcaccagaa gtatgacccg
    19321 ttttcaggcc gtcaacattc atggttttat cccacaacaa cccgttacgg ttttgctggg
    19381 tgataccgtt ccaggtgaga cttttctcac tgtacatatg ataaaactcg ggctcgccgt
    19441 ggatgatagc gcgagaaagc acagctaaat cataagccga gctatgctgg ccaggtgcat
    19501 ccagaccatg cactgtttca aaatgcgtat ccttgagatg cagcttctcg gcatagttgt
    19561 tcatcatttc aacaaactgc cgttgcccac cggcaatata gtcagccaga gcaacacaag
    19621 cgtcatttcc ggaatccaca attaaaccac ggcttaaatc acgtaccgat acgcgatcgc
    19681 cctctttcaa aaacatcagt gaagaaccga caaacaccgg attatctttc gcccacgcat
    19741 cgcgccccac ggtgacaata tcgtctggcg taatgcgatg actatcgata gcgcgatcca
    19801 cgacataacc cgtcatcagc tttgtcaggc tggcgggatt gcgctgttga tgctcattac
    19861 ccgcggtgag gatctgaccg gtggtgtaat ccatcaatac ccaggacccg gcatgaatct
    19921 ctggaggctg aggtgaaaaa ggaatgtttt ccgccgcaaa accagacgat aagttaaaaa
    19981 cgaacaaaga agcagcaata ataagacggc gtttcaacag caaaccctca ggagtttcaa
    20041 atagctgttc tttttacgga aatacttatg aactggctgg aataaagtgc aagaaaatgt
    20101 gactaccctc tcatttttat ctgacatgat ctgttgccac tcgctgccaa attgtggcgc
    20161 taaagctgat tagcacggtg atatttgata ctctggcaga cagcagaaat aacggattta
    20221 acctaatgat gaatgacggt aagcaacaat ctaccttttt gtttcacgat tacgaaacct
    20281 ttggcacgca ccccgcgtta gatcgccctg cacagttcgc agccattcgc accgatagcg
    20341 aattcaatgt catcggcgaa cccgaagtct tttactgcaa gcccgctgat gactatttac
    20401 cccagccagg agccgtatta attaccggta ttaccccgca ggaagcacgg gcgaaaggag
    20461 aaaacgaagc cgcgtttgcc gcccgtattc actcgctttt taccgtaccg aagacctgta
    20521 ttctgggcta caacaatgtg cgtttcgacg acgaagtcac acgcaacgtt ttttatcgta
    20581 atttctacga tccttacgcc tggagctggc agcatgataa ctcgcgctgg gatttgctgg
    20641 atgttatgcg tgcctgttat gccctgcgcc cggaaggaat aaactggcct gaaaatgatg
    20701 acggtctacc gagctttcgc cttgagcatt taaccaaagc gaatggaatt gaacatagca
    20761 acgcccacga tgcgatggct gatgtgtacg ccactattgc gatggcgaaa ctggtaaaaa
    20821 cgcgtcagcc acgtctgttt gattatctct ttacccatcg taataaacac aaactgatgg
    20881 cgttgattga tgttccgcag atgaaacccc tggttcacgt ttccggaatg tttggggcat
    20941 ggcgcggcaa taccagctgg gtggcaccgc tggcgtggca tccagaaaat cgcaatgccg
    21001 taattatggt ggatttggca ggagacattt cgccattact ggaactggat agcgacacat
    21061 tgcgcgagcg tttatatact gcaaaagccg atcttggcga taacgccacc gttccggtta
    21121 agctggtgca tatcaataaa tgtccggtgc tggcccaggc gaatacgcta cgcccggaag
    21181 atgccgaccg actgggaatt aatcgtcagc attgcctcga taacctgaaa attctgcgtg
    21241 aaaatccgca agtgcgcgaa aaagtggtgg cgatattcgc ggaagccgaa ccgtttacgc
    21301 cttcagataa cgtggatgca cagctttata acggcttttt cagtgacgca gatcgtgcag
    21361 caatgaaaat tgtgctggaa accgagccgc gtaatttacc ggcactggat atcacttttg
    21421 tcgataaacg gattgaaaag ctgttgttca attatcgggc acgcaacttc ccggggacgc
    21481 tggattatgc cgagcagcaa cgctggctgg agcaccgccg ccaggtcttc acgcccgagt
    21541 ttttacaagg ttatgcagat gaattgcaga tgctggcaca acaatatgcc gataacaaag
    21601 agaaagtggc gctgttaaaa gcgctttggc agtacgcgga agagatcgtc taatttgaag
    21661 cctccgccgc tggtacggcg gaggtcaatg ttgagtcagg ctttttgaac ggtgatactc
    21721 cacgctgcat cgccaatctg ctgataatcg gtgatggcat gcccttcttc tgccgcccac
    21781 tgtgggattg cttcagttgc ctgggtgcaa tcaaactcaa ttaccaactc atcgccgctg
    21841 gccatttctg ccagtgctgc ttttgcttct atgagtggaa acggacagac ctgcgtcacc
    21901 acatccagct ttttaatcgc catctttaca ccttacttaa tttgccgcag cagttgccag
    21961 tcgcgcctta cgctgcggtc gaacatacac aagccaggac gcagtccaga ctccgagaat
    22021 cataaataca aggccaatcc agccctgcca ggtcatcatt gcagtcataa ccagtccatt
    22081 accgataaag caaccacctg caatactggc accgaagccc atcagtacac cacctaaccc
    22141 gctacgtaat gttgtttgtg catcagctgc gcgaacgcgg aactcacggc tcgctttggc
    22201 tgcaataaat gaccccacga agatccctaa caccaggaaa acgccccagt taatgtattt
    22261 cacgtcaccc gcgaccagaa attgcagaat attggccgtt ggggaagtga ttcctaaccc
    22321 aaacatgcgt ccggttgctt cactcagggg ccaggctaaa agcgcaatca aaccgatgag
    22381 tacagcagtg acaaagggat gccagcgttt ttcaaacaga atatgagcga tcccagttcg
    22441 gcgcggcggt aaggtcgcga ctttgagttt tggcttcttc aactctttca tcaccaccca
    22501 gagcgttatc accagcaaaa cggcaaccaa cggccaaaca gacaaattaa aagtctcagc
    22561 aatagagtta tgttcagtac tgtagtgctg caaggtttga tttaaaccac tggcatgtgg
    22621 agaacgcatc accgcactca tcaccatata agtgaaaagc gcgatccaac tgccgatcaa
    22681 tccctcaccc gcgcgatacc aggtcccggt ggcacatcca cccgccagaa caattcccag
    22741 cccgaagata tacccaccta taacagtacc aagccacggg aacgcctcgg cttcgtaagt
    22801 cagtaggccc gcctgaatta acgcaaaaac ccccacgctt tgtaccgaaa ttgcaatcag
    22861 caaggcgtaa aacatgcgat tatttttcgc gatatacata tcgcgaaaac cacctgtcag
    22921 gcaaaaacgc ccacgctgca tgacaaatcc cagcagagca ccacaaatta gcccgcttaa
    22981 tatcattgaa aacataaatt aattaaccag atgaatgtta atgaggaaat tattcatgac
    23041 tggtggaatg cagaccaata accaaattct ctaaattaga acaaatggtt attaatgagg
    23101 atttcgataa aaaaaaataa aaaaaccgga gccaaatatt cattcgactc cggttgatta
    23161 ttagaaaaga tgattatgca acgtcttcgt actgcggaac tgggttacgg aagctcttgg
    23221 tgacgcaagc caggtaaatc agaccgatag cagcccaaat cagacccaga accattgagc
    23281 tttcttccag gttaacccac agcgcaccaa cggtcagcgc accacacatc ggcaggaaca
    23341 gatactggaa gtgatctttc agcgtcttat tacgcttctc acggatccag aactgcgaga
    23401 tgaccgacag gttaacgaag gtgaacgcca ccaacgcacc aaagttaatc agcgctgtag
    23461 ccattaccag gtcgaagttg attgccagca gggcaatcgc gccaaccagg atgatgttca
    23521 tcgccggagt acgccatttc gggtgtacat aaccgaagaa gcttttcggg aatacgccgt
    23581 cacgacccat tacgtacatc agacgcgcta cgcctgcatg cgccgccata ccggacgcca
    23641 gtacggtaat ggtggagaag atcagcgcgc caacctggaa cgctttaccc gcaacgtaca
    23701 gcatgatttc aggctgtgac gcatccggat ctttaaagcg agagatatcc gggaagtaca
    23761 gctgcaggaa gtaagttgca aagatgaaga tcatgccgcc aatcagcgcg gtcaggaaaa
    23821 tcgcacgcgg gatcacgcgc tctgcatctt tggtttcttc cgacaggttg ctgatgccgt
    23881 caaagccggt aaaggaaaag cacaggattg tcgccccggt aatcatcgga ataacatgcg
    23941 catcgccaga ccagaacgga cgagtgctcg ccagcgtacc agcgccttca ccttcaaata
    24001 cgccataaac aaccatgccc agaatcaccg cgatcagcac tacctgcaac acgacgatta
    24061 cagtgttgaa gttcgctacg gatttcagac tacgcaggtt aaaggcggtc ataaaggcca
    24121 ccagcgccac cacaaacatc cacgatggga tggaaggcac cagagcttca aaatagattt
    24181 tcgccagcag aatgttgatc atcggcgcga acagatagtc gagcagagaa gaccaaccca
    24241 ccataaagcc gacagtcggg ctaatggatt tctgggcgta agtatatgca gagccagcag
    24301 aaggatagcg gcgaaccagc ttcccgtagc tcagagccgt aaacaggatc gcaatcaatg
    24361 cgaacgcata ggctgtcggc acatgaccat ccgtgaggcc tgaaacgata ccaaaagtat
    24421 caaacagcgt catcggctgc atataggcaa gacccatcat tacaaccgga actaacgtaa
    24481 gcgttttacg caattccacg cgagaggtgt ttggagtaac gttatgcgac atggtcattc
    24541 tcctttacgg tgataaccgt cgcgtaagca aaaaattgcc ccattttttt ggattcctca
    24601 gcgacaacaa ctgtcgattt ttagtaaata tctatccggt acgaagcccg gcctcttggt
    24661 atgaatgatt ggtttgaagc aaaaaataac cgacgctgat gaaacgtcgg tttttagtca
    24721 ttttttgaca gcggcgcatt gtgcctaaat gggggggaaa tgacaagaga atgagaagct
    24781 tgtcataata atttttcttt aaatggctga ttttccgtca tcagattttg cataaacacc
    24841 ggcgatcgca ctatttgcta aaatttcatc ccgccaccag ccgctggcaa gccctgcggt
    24901 ctgttcgttc cagccaatcc agacgggttc aaagagagtt tgggcgacca ctttcttctc
    24961 gattaacgcg ccactatcga gaaaacgttg caccagataa cggggtaaat agccgcagcc
    25021 gaggccgcta atttgcagct ccagcttggt tttaaaatcg aaaacggtaa tggcttcttg
    25081 ttcgtcgaga agttgtgagg ccgtagaagc gcctgcctgc gcagtgtccc ccaccacaat
    25141 cgcccgatat cgcttaatga tacgacggtt taacggctct tcttcctggg ctaacggatg
    25201 atgaggcgcg accgcaaaga cttgttccag atcgcccaac cgcgagaaac caaactcact
    25261 ggaagatggt ggctcatgca tcgcgccaac aataatatcc gctcgcccct gagtcagggc
    25321 atcccaggaa ccaccgagta cgccattgat aaacttcagg cgcgtcacgc tatgatgttg
    25381 ataaaaagct tcaatcagtg gcgcaagcag agaaaaagga aacgtgtcgt ccacgccaat
    25441 caccagctcg ttttcccagc cttcatgcag tttgatcgcc tgcttttcca gttctcgcac
    25501 ggtatgcaga acttcccgcc ccttctctaa taacattttt ccggtacggg tgaatttagc
    25561 gcggtgaccg ctacgatcaa gtaattgaat attaaggtcg ctttccagct tgtgaacggt
    25621 gtaactcaga gcggacggcg tcttgtagag tttagccgac gccgccgcaa aactgccttc
    25681 tttttctaag gcatcgagga tcatcaacac atccagcagt ggtttcatac acgccccctt
    25741 gcagtgcatg cggcgtcctg ctggcgtgat cgtttactcc agcggcatta ccagcggatc
    25801 gggatactgg tattcaaatc ccagttcatt acaaatccga ctgccatcaa taatcttacc
    25861 tttgccgctg tccagactat ttctgaactg cggaggttcc agccccagta aacgggccat
    25921 ctgcggatag aaaacattac gcgcagggtg agctggcgca catatattat agatgtgtcc
    25981 gcctttaggt gcctgtaaca acagagtgat agcgccaata acatcttcta aatggactaa
    26041 attaacacca tgttcaccat caggcgcggt ttttccggca aagaagcgtc cgggatgacg
    26101 tcccggtccg accagccccg caagacgcag aatatcgacc gaagtaccgg gtaaattgtg
    26161 cagccagtct tcgagttctt ctaatactcg tccactgttg gtcaccggat tacgcggggt
    26221 ggtttctttc accgtgcctt gcgcgtcgcc atagacagac gttgagctgg taaaaataat
    26281 gcgaggaata cgatgggcca gcgcgctatc cactaactct tgtaccgctt gtaaatagaa
    26341 ctcatcgccg gggccgctac gacgtgccgg aagcgtaatg accagcgcat cggcatccat
    26401 cagggcatcc agatcgtcag aatcgcaaac taactcaggc tccatgcgaa gcagatagct
    26461 atcaatgcca ctcattcggg ccgcttcgac accatcttgt gtggttttac tcccggtgac
    26521 ttgccagcct cgcgctgaaa gtgacatcgc cagcggcatg cctaaccacc ctaacccgac
    26581 aattgcgacc tttttcatcc gttatctcct gatacacctt agatctataa ggctacgcta
    26641 gcgtatcaaa actgacaatt cattctatga atgaatctgt tcaataatga taacgacatg
    26701 ctgcaatgag cagtgaatcg tcggtaaccg cgtataccag acggtgctcc tctgtaatgc
    26761 gtcgggacca gaaacctgac aaattatgtt tcaggggttc tggcttcccc ttaccttcaa
    26821 atggcgttct gcgggtatct ttgataagtt cattgatctt tttaacaatt cgcttatctg
    26881 tttcctgcca gtacagataa tcatcccatg attcctcaga ccagattagt ttcactcaat
    26941 aatgtccttt tctgttcctt tgcctgattt caggctatcg attgagtcca tcaatctccg
    27001 ggcgttagcg ggggagcgca gtagataagc cgtctcttcc agcgagttgt attcttcgag
    27061 tgacatcaga acacaagcct ctccattctg acgagtaata aggatcgggg catgatcttc
    27121 aacggctttc atcattgttg ccgacaaatt ctgacgcgct tcgctgtagc taattgtacg
    27181 catgtcaatc tcctcttttg tacagtttat tgtacaatga tgggcgttaa ttaactattt
    27241 attaattagt ttgtagatca aggtattgtc agtgagacga aaatccaggc ttcgctattt
    27301 ttggtgccgt cagcttagag gacagtcctc ttagccccct cctttccccg ctcattcatt
    27361 aaacaaatcc attgccataa aatatataaa aaagcccttg ctttctaacg tgaaagtggt
    27421 ttaggttaaa agacatcagt tgaataaaca ttcacagaga cttttatgac acgcgttcaa
    27481 tttaaacacc accatcatca ccatcatcct gactagtctt tcaggcgatg tgtgctggaa
    27541 gacattcaga tcttccagtg gtgcatgaac gcatgagaaa gcccccggaa gatcaccttc
    27601 cgggggcttt tttattgcgc ggttgataac ggttcagaca ggtttaaaga ggaataacaa
    27661 aatgacagac aacactcgtt tacgcatagc tatgcagaaa tccggccgtt taagtgatga
    27721 ctcacgcgaa ttactggcgc gctgtggcat taaaattaat cttcacaccc agcgcctgat
    27781 cgcgatggca gaaaacatgc cgattgatat tctgcgcgtg cgtgacgacg acattcccgg
    27841 tctggtaatg gatggcgtgg tagaccttgg gattatcggc gaaaacgtgc tggaagaaga
    27901 gctgcttaac cgccgcgccc agggtgaaga tccgcgctac tttaccttgc gtcgtctgga
    27961 tttcggcggc tgccgccttt cgctggcaac gccggttgat gaagcctggg acggcccgct
    28021 ctccttaaac ggtaaacgta tcgccacctc ttatcctcac ctgctcaagc gttatctcga
    28081 ccagaaaggc atctctttta aatcctgctt actgaacggt tctgttgaag tcgccccgcg
    28141 tgccggactg gcggatgcga tttgcgatct ggtttccacc ggtgccacgc tggaagcaaa
    28201 cggcctgcgc gaagtcgaag ttatctaccg ctcgaaagcc tgccttatcc agcgcgatgg
    28261 tgaaatggaa gaatccaaac agcagctgat cgacaaactg ctgacccgta ttcagggtgt
    28321 gatccaggcg cgcgaatcaa aatacatcat gatgcacgca ccgaccgaac gtctggatga
    28381 agtcatcgcc ctgctgccag gtgccgaacg cccaaccatt ctgccgctgg cgggtgacca
    28441 acaacgcgta gcgatgcaca tggtcagtag cgaaaccctg ttctgggaaa cgatggaaaa
    28501 actgaaagcg ctgggtgcca gttcaattct ggtcctgccg attgagaaga tgatggagtg
    28561 atcgccatga gctttaacac aatcattgac tggaatagct gtactgcgga gcaacaacgc
    28621 cagctgttaa tgcgcccggc gatctccgct tctgaaagca ttacccgcac tgttaacgat
    28681 attctcgata acgtgaaagc acgcggtgat gacgccctgc gggaatacag cgcgaagttt
    28741 gataaaacca cggttaacgc gctgaaggtg tctgctgagg agattgccgc cgctagcgaa
    28801 cgcctgagcg acgagctaaa acaggcgatg gcggtggcag taaaaaatat tgaaaccttc
    28861 cacactgcgc aaaaactgcc gccggtagat gtagaaacgc agccaggcgt gcgttgccag
    28921 caggtcacgc gcccggtcgc ttcagttggg ttgtatattc ctggcggctc cgccccgctc
    28981 ttctcaacgg tattaatgct ggcgactccg gcgcgtattg cgggctgtaa aaaagtggtg
    29041 ctgtgctcac cgccgccgat tgccgatgag atcctttatg cggcgcagct gtgcggtgtg
    29101 aaggacgtgt ttaacgtcgg cggcgcacag gccattgccg cgctggcgtt tggtacggaa
    29161 tctgtgccga aagtggacaa aatcttcggg ccgggtaacg cctttgtcac cgaagcgaaa
    29221 cgtcaggtga gccagcgtct ggacggtgcg gctatcgata tgcccgcagg cccgtcggaa
    29281 gtgttggtca ttgccgacag cggcgcaacg ccggatttcg tggcttctga tttgctttct
    29341 caggctgaac acggcccgga ctcacaggtg attttactga cgcctgacgc tgatatggcg
    29401 catcaagttg ccgaagccgt cgaacgccag ttagcagaac tgccgcgtgc cgaaaccgcc
    29461 cgccaggcac tgaacgccag ccgcctgatc gtgactaaag atttagcgca gtgcgtagag
    29521 atctccaacc agtacggccc ggagcacctg atcatccaga cccgcaacgc ccgcgatctg
    29581 gtcgatggca tcaccagcgc cggttcggta tttcttggtg actggtcacc ggaatcggca
    29641 ggtgattacg cctccggcac caaccacgtt ctgccgactt acggttacac cgccacctgt
    29701 tccagcctcg ggctggcaga tttccagaag cgtatgaccg tgcaggaact gtcgaaagaa
    29761 ggtttctccg cgctggcttc gaccattgaa acattggccg ccgccgagcg cctgaccgcc
    29821 cataaaaatg ccgttacttt gcgtgttaac gcccttaagg agcaagcatg agcaccgtga
    29881 ctattaccga tttagcgcgt gaaaacgtcc gcaacctgac gccgtatcag tcggcgcgtc
    29941 gtctgggcgg taatggcgac gtctggctga acgccaacga ataccccact gccgtggagt
    30001 ttcagcttac tcagcaaacg ctcaaccgct acccggaatg tcagccgaaa gcggtgattg
    30061 aaaattacgc gcaatatgca ggcgtaaaac cggaacaggt gctggtcagt cgtggcgcgg
    30121 acgaaggtat tgaactgctg attcgcgctt tttgcgaacc gggtaaagac gccatcctct
    30181 actgcccgcc aacgtacggc atgtacagcg tcagcgccga aacgattggc gtcgagtgcc
    30241 gcacagtgcc gacgcaggac aactggcaac tggacttaca gggcatttcc gacaagctgg
    30301 acggcgtaaa agtggtctat gtttgcagcc ccaacaaccc gacagggcaa ctgatcaatc
    30361 cgcaggattt tcgcactctg ctggagctaa cccgcggaaa agccattgtg gttgccgatg
    30421 aagcctatat cgagttttgc ccgcaggcat cgctggctgg ctggctgacg gaatatccgc
    30481 acctggctat tttgcgcaca ctgtcgaaag cttttgctct ggcgggcctt cgttgcggat
    30541 ttacgctggc aaacgaagaa gtcatcaacc tgctgatgaa agtgatcgcc ccctacccgc
    30601 tctcgacgcc ggttgccgac attgcggccc aggcgttaag cccgcaggga atcgtcgcta
    30661 tgcgcgaacg agtgacgcaa attattgcag aacgcgaata cctgattgcc gcactgaaag
    30721 agatcccctg cgtagagcag gttttcgact ccgcaaccaa ctacattctg gcgcgcttta
    30781 aagcctccag cgcagtgttt aaatctttgt gggatcaggg cattatctta cgtgatcaga
    30841 ataaacaacc ctctttaagc ggctgcctgc gaattaccgt cggaacccgt gaagaaagcc
    30901 agcgcgtcat tgacgcctta cgtgcggagc aagtttgatg agtcagaagt atctttttat
    30961 cgatcgcgat ggaaccctga ttagcgaacc gccgagtgat tttcaggtgg accgttttga
    31021 caaactcgcc tttgaaccag gcgtgatccc ggaactgctg aagctgcaaa aagcgggcta
    31081 caaactggta atgatcacca atcaggatgg tctgggaaca caaagtttcc cgcaggcgga
    31141 tttcgatggc ccgcacaacc tgatgatgca gatcttcacc tcgcaaggcg tacagtttga
    31201 tgaagtgctg atttgtccgc acctgcccgc cgatgagtgc gactgccgta agccgaaagt
    31261 aaaactggtg gaacgttatc tggctgagca agcgatggat cgcgctaaca gttatgtgat
    31321 tggcgatcgc gcgaccgaca ttcaactggc ggaaaacatg ggcattaatg gtttacgcta
    31381 cgaccgcgaa atcctgaact ggccaatgat tggcgagcag ctcactaaac gagaccgtta
    31441 cgctcacgta gtgcgcaaca ccaaagagac acagattgac gttcaggtgt ggctggatcg
    31501 cgaaggtggc agcaagatta acaccggtgt tggcttcttt gatcacatgc tggatcagat
    31561 cgccacccac ggtggtttcc gtatggaaat caacgtcaaa ggcgacctct atatcgacga
    31621 tcaccacacc gtcgaagata ccggcctggc gctgggcgaa gcgttaaaaa tggctcttgg
    31681 cgacaaacgc ggtatttgcc gctttggttt tgtgctaccg atggacgaat gccttgcccg
    31741 ctgcgcgctg gatatctctg gtcgcccgca cctggaatac aaagccgagt ttacctacca
    31801 gcgcgtgggc gatctcagca ccgagatgat cgagcacttc ttccgttcgc tctcatacac
    31861 catgggcgtg acgctacacc tgaaaaccaa aggtaaaaac gatcaccacc gtgtagagag
    31921 cctgttcaaa gcctttggtc gcaccctgcg ccaagccatc cgcgtggaag gcgacaccct
    31981 gccctcgtcg aaaggagtgc tgtaatgaac gtggtgatcc ttgataccgg ctgcgccaat
    32041 ctgaactcgg tgaagtcagc cattgcgcgc cacggttatg aaccaaaagt cagccgtgac
    32101 ccggacgtgg tgttgctggc cgataaacta tttttacccg gcgttggcac tgcgcaagca
    32161 gcgatggatc aggtgcgtga gcgcgagctg tttgatctca tcaaagcctg tacccaaccg
    32221 gtactgggca tctgcttagg gatgcaactg ctgggtcggc gcagcgaaga gagcaacggc
    32281 gtcgacttgc tgggcatcat tgacgaagac gtgccgaaaa tgaccgactt tggtctgccg
    32341 ctgccgcata tgggctggaa tcgcgtttac ccgcaggcag gcaaccgcct gtttcagggg
    32401 attgaagacg gcgcgtactt ttactttgtt cacagttacg caatgccggt caatccgtgg
    32461 accattgccc aatgtaatta cggcgaaccg ttcaccgcgg cggtacaaaa agataacttc
    32521 tacggcgtgc agttccaccc ggagcgttct ggtgccgctg gcgctaaatt gctgaaaaac
    32581 ttcctggaga tgtgatgatt attccggcat tagatttaat cgacggcacc gtggtgcgtc
    32641 tccatcaggg cgattacggc aaacaacgcg attacggtaa cgacccgctg ccgcgcttac
    32701 aggattacgc cgcgcagggt gccgaagtgc tgcatctggt ggatctgacc ggggcaaaag
    32761 atccggcaaa acgtcaaatc ccgctgatta aaaccctggt cgcgggcgtt aacgttccgg
    32821 tgcaggttgg cggcggtgtg cgtagcgaag aagacgtggc ggcgttactg gaagcgggcg
    32881 ttgcgcgcgt ggtggttggc tccaccgcgg tgaaatcacc agaaagggtg aaaggctggt
    32941 ttgaacgttt cggtgccgat gccttagtgc tggcgctgga tgtccgtatt gacgagcaag
    33001 gcaacaagca ggtggcggtc agcggctggc aagagaactc gggcgtttca ctggaacaac
    33061 tggtggaaac ctatctgcct gtcggcctga aacatgtgct gtgtaccgat atctcgcgcg
    33121 acggcacgct ggcaggctct aacgtctctt tatatgaaga agtgtgcgcc agatacccgc
    33181 aggtggcatt tcagtcctcc ggcggtattg gcgacattaa cgatgttgct gccctgcgag
    33241 gcactggcgt gcgcggcgta atagttggtc gtgcattact ggaaggtaaa ttcaccgtga
    33301 aggaggccat cgcatgctgg caaaacgcat aatcccatgt ctcgacgttc gtgatggtca
    33361 ggtggtgaaa ggcgtacagt ttcgcaacca tgaaattatt ggcgatattg taccgctggc
    33421 aaaacgctac gccgaagaag gtgcagacga actggtgttc tacgatatca ccgcttccag
    33481 cgatggccgt gtggtagata aaagctgggt atctcgcgtg gcggaagtga tcgacattcc
    33541 gttctgcgtg gcgggtggga ttaagtctct ggaagatgcc gcgaaaattc tttcctttgg
    33601 cgcggataaa atttccatca actcccctgc gctggcagac ccaaccttaa ttactcgcct
    33661 ggcggatcgc tttggcgtgc aatgcattgt ggtcggcatt gatacctggt acgacggcga
    33721 aaccggtaaa tatcatgtga atcaatatac cggcgatgaa agccgcaccc gcgtcactca
    33781 gtgggaaaca ctcgactggg tacaggaagt gcaaaaacgt ggagccggag aaatcgtcct
    33841 caacatgatg aatcaggacg gcgtgcgtaa cggttacgat ctcgaacaac tgaaaaaagt
    33901 gcgtgaagtt tgccacgtcc cgctaattgc ctccggtggc gcgggcacca tggaacactt
    33961 cctcgaagcc ttccgcgatg ccgacgttga cggcgcgctg gctgcttccg tattccacaa
    34021 acaaataatc aatattggtg aattaaaagc gtacctggca acacagggcg tggagatcag
    34081 gatatgttaa cagaacaaca acgccgcgaa ctggactggg aaaaaaccga cggactaatg
    34141 ccggtaattg tgcaacacgc ggtatccggc gaagtgttaa tgctgggcta tatgaacccg
    34201 gaagccttag acaaaaccat cgaaagcggc aaagtcacct tcttctcgcg cactaaacag
    34261 cgactgtgga ccaaaggcga aacgtcgggc aatttcctca acgtagtaaa tattgcgccg
    34321 gactgcgaca acgacacgct actggtactg gcgaatccta tcggaccaac ctgccacaaa
    34381 ggcaccagca gctgcttcgg cgacaccgct caccaatggc tgttcctgta tcaactggaa
    34441 caactgctcg ccgaacgcaa atctgccgac ccggaaacct cctataccgc caaactgtat
    34501 gccagcggca ccaaacgcat tgcgcagaaa gtgggcgaag aaggcgtaga aaccgcactg
    34561 gcagcgacgg tacatgaccg ctttgagctg accaacgagg catcggactt gatgtatcac
    34621 ctgctggtgc tgttgcagga tcaggatctg gatttaacga cggtaattga gaacctgcgt
    34681 aaacggcatc agtgagttgc agggtaagcg gatgcgatgt tgttgtcgca tccggcaaaa
    34741 aaaaagggca aggtgtcacc accctgcccg ttttctttaa agctgcaaag attacttcgc
    34801 gttataatta cgcagagcgt tacgccccag cacaatcccc gcgccaacca tgccgcccag
    34861 cagcaccgcc agaatcaagg taattgcctt tttcgggcta tcgcgacgaa taggtaacgt
    34921 cggtttcatc acataacggt aagcatgaat atcaagatca tcaaccttta agttttcaat
    34981 atccaacagg ttttgacgtg tctgatagta gtttggtgag aacaccaacg gacgggtcgc
    35041 ctcatgctta atcatcgact ccagcgcttc gctccccaaa aggaacaacg tatcttgtgt
    35101 cacatcttca gtctgctgaa cctgtggctt tgccacctgc gcctgattcg catactgcaa
    35161 cgcttcctga atctgacgga tacgcagatc tttctgctcc tgcgcgacta cttcctgcgt
    35221 tcttaaagag tcctgcaagt ttttccgtcc cagagcaatg ttgtctttga gatccttttc
    35281 tagctcttga ttcactttat catcaacttg ctgaatgtat tgggccaact tcatttgtgc
    35341 tccctctgca gtttgcccaa cataagaaac agtcaatggt aattgctggt tcttaacaga
    35401 aggttcgatg gtaagttttt ctggttcttc ctgattatcc agcgtttctg ctaatgcaga
    35461 gaaggcagaa ctgaagcgac caattaacgt ctcctgtaaa tccgatactt tcggtgcagc
    35521 ctgaccatag ataacattca tggcattgtt atagccagca atttgcccca cgtcgggctg
    35581 agtgataatt gctgttgacg tccatttctc cttcgctact gccaaatatc caatagccag
    35641 ggcaatagcc acaatgacgg aaatgatgat tgtcatcttg ccacgccaca actgcactag
    35701 taaatcaatc aaatcaatct gttccgggtc atggttttgc ccagaaacat tattattttc
    35761 tactctcatc attaccctaa ctgacggaaa tttccaagtc cgcatagttt agcgataatc
    35821 ctagtgaatg ctataggata agtgataaaa ataatgaatt attggggagg taacaaaaga
    35881 caatcagggc gtaaatagcc ctgataacaa gatgttagtc gctgccaaag agatcgcggg
    35941 tgtatacctt atctgccaca tctttaagct cttctgccat tcggttagag ataatgacgt
    36001 cggcttgttg tttgaagatg gcgagatcac gttccagccg agagttgaag aatgagtctt
    36061 ctttcatcac tggctcgtag atgatcactt caacgccttt cgccttgata cgcttcataa
    36121 tcccctgaat agaagacgca cggaagttat ctgaaccgct cttcataatc agacgataaa
    36181 tacccaccac ttgcggtttg cgtgacaaaa tggcatcggc aataaaatcc ttacgcgtgc
    36241 ggtttgcatc gacaattgcc gagatcaggt tattcggcac agactgatag ttcgccagta
    36301 actgcttggt atctttcggc aagcaataac caccataacc aaacgacggg ttgttgtaat
    36361 ggttgccaat acgcggatcg agacaaacgc cttcgataat ctggcgagta ttcagaccta
    36421 aactttctgc gtagctatcc agttcgttaa agtatgccac gcgcatcgct aggtaggtgt
    36481 ttgcaaaaag tttaatcgct tctgcttcgg tcgaatcggt gaacagggtc gggatatttt
    36541 gcttaatcgc gccttcctgt aacaacgcag caaaacgttc tgcgcgttct gaacgctcac
    36601 cgatgacaat acgtgaagga tggagattat cgtaaagggc tttaccttca cggagaaatt
    36661 ccggagagaa aataatattt tcagtacgat atttcttatg catcgcttcg gtaaaaccaa
    36721 cgggaaccgt tgatttgatg accataaccg cataaggatt tatctcaact acgtctttaa
    36781 ttactgattc tacactggat gtattgaaat aattagtttt aggatcatag tcggttggag
    36841 tggcgatgat gacataatca gcatcccggt aggcttcatt tttatctaat gtggcattaa
    36901 agtgtatttt atctgattgc aaaaactgct gaatttcctt atcaacaata ggagatatcc
    36961 gatcattcag catagcaacg cgtgacggta aaatatctaa tgccacaacc tcatgatttt
    37021 gtgcgattag aagcccgttt gacaagccta catagccagt accggaaatg gtgattttca
    37081 tttcatgctc tcagaattaa cttaactgtg aatcatgatg tttttagcat cctgataaga
    37141 gctaaatgtt ttaacgctac gttattagct ggtcaacgct acgaaaaaca attcagattt
    37201 tctttataag aatagctgat atttattaag ttaatattaa gcagtgaaat ttagtctata
    37261 agataaatgg caaaaaaaaa gcccggtgca atatacgccg ggcctcaatt ttactgttgg
    37321 ttaaatcaga ttaatccagc cattcagtat ggaacacacc ttctttatca atgcgcttat
    37381 aagtatgtgc accgaaatag tcacgctgtg cctggatcag gttcgcaggc agaacagcgg
    37441 cacggtagct atcgtaatag gcaaccgcag cggcgaaggt cggaaccggg ataccgttct
    37501 gtactgcata agcaacgaca tcacgcagcg cctgctggta gtcatcggca atttgcttga
    37561 agtacggagc cagcagcagg ttagcgatct gcggattttc ggcatatgca tcggtgattt
    37621 tctgcaggaa ctgcgcacgg atgatgcagc cagcacggaa aatcttcgcg atttcgccgt
    37681 agttcagatc ccagttgtat tcttcagacg cagcacgcag ctgagagaag ccctgagcgt
    37741 aagaaacgat tttgcccaga tacagcgcac ggcgaacttt ttcgatgaac tcagccttgt
    37801 cgcctgctga ctgagcttgc ggaccagaga gaactttaga tgcggcaacg cgctgatctt
    37861 tcagagaaga gatataacgt gcaaacacag actcggtaat cagcgacagc ggttctccga
    37921 gatccagcgc gctctggctg gtccatttac cggtaccttt gttagccgct tcatccagga
    37981 tcacatcaac caggtagtta ccgtcttcat cttttttagt gaagatatct ttggtgatgt
    38041 cgatcaggta gctgctcagt tcaccgttat tccactcggt aaaggtctgt gccagttctt
    38101 cgttagagag attcaggccg cctttcagga gagaataggc ttcagcaatc agttgcatat
    38161 caccgtattc aataccgttg tgaaccatct tcacataatg gcctgcgcca tcagcaccga
    38221 tataggtaac gcacggttcg ccatcttcag caacggcggc gattttggtc aggatcggcg
    38281 caaccagttc ataggcttct ttctggccac caggcatgat ggacggccct ttcagcgccc
    38341 cctcttctcc gccggaaaca ccggtaccga tgaagttaaa gccttcggca gaaagctcac
    38401 ggttacgacg aatggtgtcc tggaagaagg tgttaccacc atcgatgatg atgtcgcctt
    38461 tatcgagata tggtttgagc gaatcaatag cagcatccgt gcctgcacct gctttcacca
    38521 ttaacaggat gcgacgaggc gtttccagag attcaacaaa ctctttcacc gtatagtaag
    38581 gaaccagttt cttgcctgga ttttcggcaa tcacttcttc cgtcttttca cgggaacggt
    38641 tgaaaataga gacggtataa ccacggcttt cgatgttgag cgcaaggttg cgccccatca
    38701 ctgccatacc gactacgccg atctgttgct ttgacattgt ttactcctgt cagggggtgg
    38761 tcaccgccga gtgtgcggct taaatgtggt atagatgtta actcactcat agagatggtg
    38821 aatagcatta cctttgctat accacactga atatctcacc ttttccgtct tttaaaactt
    38881 atttatatga aattactgaa ttacttgttc agtaactcaa gaataagttt tgtcttttct
    38941 tccatcagcg gcacatcacc gcgagattcc acattcaacc gcaccaccgg ttcggtgttg
    39001 gaggtgcgca ggttaaagcg ccagtcggca aaggtcatgc tgatgccatc ggtgcgatcc
    39061 accgccagcg cctcgcggct aaaatgctgt tccacgcggt taatcgcctc aacgggttgc
    39121 gccagtttgc tgttgatctc accgcttgcc ggaaacgctg ccatccggtc gcgcaccagt
    39181 tcgcccagcg tttttccttt caggcacacc agttcggcga ccagcagcca cgggatcatg
    39241 ccgctgtcgc agtaagcgaa atcacggaag taatggtggg cgctcatttc accaccgtag
    39301 atggcgtctt ccttgcgcat ccgttcttta ataaaggcgt gtccggtttt cgacatcacc
    39361 ggcgtgccgc ctgcggcagt caccacatca acggtgttcc aggagagacg cggatcgtgg
    39421 atgatcttcg cgccgggatt tttttcgagg aatgcttctg ccagcaggcc gacaatgtag
    39481 tagccctcaa taaactgccc tttttcgtca aacaggaagc agcggtcaaa atcgccatca
    39541 aaagcaatgc ccatatccgc accgtgtttg atgaccgcat tgcgggtgtc gtcgcggcat
    39601 tccggcagta gcgggttagg aataccgttg gggaaattgc cgtccggcgt gttgtgcact
    39661 ttgattaatt ccacgggtgc gccaagggct ttaaagcggg cttcgatggc gtccaccacc
    39721 ggacccgctg cgccattccc ggagttaatc accagcttga gcggcgtgag gtttttgaca
    39781 ttgatataac cgaacaggtg atcaacgtaa gcgtcacgca gattgatttg ctgatagcga
    39841 ccgcgtttgg tttcatcaac gggaggaaag tcgttggctt ctgccagacg ctggacatcg
    39901 cgcagtccgg tatcaccgct gatcgggcga gccccttcgc gcaccagttt catgccgttg
    39961 taatccatcg ggttatggct ggcggtaact tcaatgccgc catccacgcc gagatggaac
    40021 gtggcgaaat agatctcttc ggtgccggac atgccaatat ccagcacatc gacgcccgca
    40081 tcctgtaaac ctctcgccag cgccagtttt aaggtttcgc tggtgaggcg gacatcaccg
    40141 cctaacacaa tggttttcgg tttgagaaat tcgccatagg cgcgaccaat gcgccaggcg
    40201 atatcttcat tcagttcttc gcctaatttc ccgcgaatat cataggcttt aaagcaggtt
    40261 aattttttca tgtcgttacc ctttttcagg caatagttgg ccctgaccga aacggccaat
    40321 tttgtttttg tcgttattta catctcggtt tcgcattcat tgcctgatgc gacgtttaca
    40381 cccgtccgta gcgatccgcg aaacgcacca catcatcctc ttcgagataa gagccggagc
    40441 gcacttcaat taaatcgagc ggaattttcc ccgggttttc caggcaatgc atcgccccca
    40501 gcggaatata aatggactcg ttttcaccaa gcagtttgat atcaccatca atggtgactt
    40561 ttgccgttcc cgcgacaacc acccagtgtt ccgcgcggtg atggtgcatc tgtaccgaca
    40621 agccctcgcc cggtttcacg gtgatgcgtt tcacctggta gcggtcgccc gcgtcgatag
    40681 agtcatattt gccccacggg cgatagactt cgcgatgcac ccgatgctca tggcgaccat
    40741 cggctttgat ctgctcgacc acttttttca catcctgcac cgcgttacgg tcggcaatca
    40801 gcaccgcatc tttggtctgc accactacca gatctttcac cccgacggtg gttacgaggc
    40861 cagattcggc gtacacatag ctgttttcag ttttgtgatt aatcacatcg ccgtggcaaa
    40921 cgttgccctc ggcggtgtgg gcgctaattt cccataatga ggaccaggag ccgacatcgc
    40981 tccagcccgc atccatcggc accacaacgg catctgccgt acgttccatg accgcgtaat
    41041 ccaccgactc ttccgggcag gcgagaaacg cttcttcatc cacacgaata aaatcgagat
    41101 ccggatcgac ggcgctcatc gctttttcac aggcatcgag aatatccgga cgatattttt
    41161 tcagttcttc gagatagcgt ccggcgcgga acaggaacat gccgctgttc cagtaatatt
    41221 cgccgcttgc cacataggcc tgggcggttt ccagattcgg tttttcgaca aactgcgcca
    41281 cttcaaaggc caccgtatcc tgctcacccg cagacacttc accgcgacga atatagccat
    41341 aaccggtttc tggtagatcc ggcacaatgc cgaaggtcac cagcttgccc gcttcggcat
    41401 atggcatggc attacgcacg gcggcacgga acgcgtcttc atcggcaatc acatgatccg
    41461 ccgccaatac cagcattaac gggtcgctct ccgggctatg acgttttgcc gccagcgccg
    41521 ccagcgcaat ggcaggtgcc gtgtttcgcc ctgccggttc gagaataatg ttctcggtaa
    41581 gtttgttcag ttgacgcagc tgttccgcga caataaagcg gtgctgctca ttgcaaatca
    41641 ccaccgggct ttcgcactcc acgccgttca ggcggcagat ggtggtttgc agcatggtga
    41701 gatcgccttt caggcataaa aactgcttgg ggtaaagtac gcgggaaagc ggccataagc
    41761 ggctaccgga gccacctgcc atcacaactg gatagagttt cgactgcgcc ataattatcc
    41821 ccgaatatca tttataaatt gacgtaacac gttctcttta tcgagtgtgc gttcggcata
    41881 ttcacgtgcc accgtgttgt gtttgggcag caggagcgcc tgacggatcc ccgccaccag
    41941 cgcctcgacc gattccggtt ctacgcaaac cgcaatgccc ggaaaggttt cgcaaagctg
    42001 ccccagttct gtgtgggcct ctgcggtaat caccgcgtta ccgcctaccg ccagaatatt
    42061 ggtcagtttc gacggcaata cggcatctgc cgcgccgcgt ttttgcacca ccagatggca
    42121 atcgcccatc ttcagcagtg cgggtaaagc gtcatacgat tgcagcggga aaaattgcat
    42181 gttgcgcagt ccacgctgct gcgccatttt ttccagccgc gctttgccgc cgccctgccc
    42241 gacaatggca aaaatcagcg gttcatcgcg cagacgatcg gcagcttcaa taacgttttc
    42301 cagcccctgc ttttcaccaa tattgccgga gtaaagaatg atttttttgt tatccggcag
    42361 gtcaagctgg ttacgaaggg cgtcaacatc ggcatctgca acatgctgaa aacgggcaat
    42421 ttccgaccag ttggggaaga agatgacgtt ttccgccgcc acgccttttt cgatggcttt
    42481 attcatcatc gaacgcgaaa tcgtggagac gttatcgacg ttatgcagtc cgctacgttc
    42541 gaacgccgtt gccagctgtg ccactttgct gcctttgcct tttccggcaa ggcccagccc
    42601 cagcatggcg tccacttcgt aatcctgaat atgcagcacg gtacgcgcac cagaaagttt
    42661 cgccagcagg cgcattcccg gcgtgcaaaa cagcgttggc accacgccga taatgcgatc
    42721 cggcttccag cgacgttgcg ccatcagagg aaagaaactg ctgacggcaa aactgccgag
    42781 atgtaacagg cgtttcaggg tgctcggctg ttttggcacg tacagcgggc agcgccacac
    42841 cgtggcggcc ccctcttctc gtttgtaccg ccaggcggaa tagttctcgc ccacctgcca
    42901 ctgcgggtag taaggcggtg cggtaataac ccgcacctca tgaccttgtg ccgccagcca
    42961 ttccaccatc tcgccggtgt atttgccgat accggttaac tccggcgagt agttaatgcc
    43021 gtagaccagt attttcataa tccgggtact ccggcacgct tctcagcgag aaaataggcg
    43081 cggctgttag cgtgaacatc gttactggcg agcagcgcgt ccggcgtcag ccagcggtaa
    43141 tcgtcatgct gctcatccgg cagtatcagc tcttcttcag cgactctgaa gcgaaaaccg
    43201 agcaccacat agtgagtggt gaaatccgtg ccggagaagt tatcgtcata aaagtgctgc
    43261 cagacaccgt aaaactggcc tgctgttatc ggcagacgca gccccagttc cgccatcgtc
    43321 agccgctcga atgcggcttc cagcgtttcg tctttctgca cgcgccctcc cggcacaaac
    43381 cagtaaccct gcgccgggcg gttggttctt ttgccaagca gaaactcacc gcgactgttc
    43441 tcgacaataa agtcgagaga gacaagcggg gtggagcgca ctaccgtggc aaagtcttcc
    43501 tgacgtaaaa acatcattac ccccgaaagc ggtcttgatt ctcaaggaac cactggtaag
    43561 tgctggcaag ccccgcttcc agtgagattt cgtgatacca gccaagctga tgcaggcgcg
    43621 tcacatccag cagtttgcgc ggcgtaccat ccggtttgct ggcatcaaaa accacccgac
    43681 ctttgtaacc caccactttg gcgatggttt gcgccagctc gcggatagtg cagtcaacgc
    43741 ccgtgccgac gttaatgtgc gacagcatcg gctgggtgtt ctccagccag acttcgtgcg
    43801 ccagctccat gacatgaatg ctcgccgccg ccatatcatc gacgtgcagg aattcacgca
    43861 tcggtgtacc gctgccccat accaccacgt ccggtgcatt ctgtgccgtc gcctcgtgga
    43921 agcgacgcag caatgcaggg atcacatgcg aattactcgg gtggaagttg tcatgcgggc
    43981 catacaggtt ggtcggcatg actgagcggt aatcgcgtcc gtactggcgg ttgtatgatt
    44041 cgcacagttt gatcccggcg attttggcaa tggcgtaaag ctcgttggtc ggctccagcg
    44101 tgccctgcaa taactcgctt tctgccatcg gctgttttgc cagtttcggg tagatacagg
    44161 acgatccaag aaacagcagt ttgttcacgt cgttctgatg cgcggcgtga atgatgttgc
    44221 tctcaatcat catgttctgg tagatgaaat ccgccggata ggtgttgttg gcaacaatgc
    44281 cgcccacttt cgccgccgcc agatagacct ggtcaatgcg ttcgctggca aagaaatcat
    44341 gcaccgcgcg gctgtccagc aggttcagct cgtcgcgggt gcgtaatacc agttccacat
    44401 caccgcgctg ttcgagctgc cgcctgatgg cagaaccgac catcccacga tggccagcga
    44461 taaaaatgcg ttgtttactc atgcttatga ctccagcgcg atcgccacgt cgtagccgtg
    44521 agatttcagc agagagtgtt ttttcgccgc ttcgaggtca ttagccacca tttcagacac
    44581 catctctctg agggtgattt ccggtttcca gcccagtttt tcgtgcgctt tggtcgggtc
    44641 gccgagcagc gtttcaactt cagccggacg gaagtaacgc ggatcaacgg cgataatcac
    44701 atcacccggt ttaacgcccg gcgcgtcatg cccggtgacg gaaaccacaa tgcccttctc
    44761 ttcaacaccc gtgccttcaa agcgcagttt gatgcccagc tgtgccgctg ccatttccac
    44821 gaactgacgc acggagtact gaacgccggt cgcgataacg aaatcttccg gctgttcctg
    44881 ttgcagcatc atccactgca tttttacgta gtctttggcg tggccccagt cacgcaggga
    44941 atccatattg ccgaggtaca ggcacgactc cagcccctgg gcgatgttgg cgattgcgcg
    45001 ggtgattttg cgggtaacga aggtttcgcc gcggcgcggg gattcatggt tgaagagaat
    45061 tccgttacaa gcgtacatgc cgtaggattc gcggtagtta acggtgatcc agtaggcgta
    45121 cagtttggcg accgcatacg gagatcgcgg gtagaacggc gtggtttctt tctgcggaat
    45181 ttcctgcacc agaccataca gttcagaggt ggaagcctga tagaaacgag ttttcttttc
    45241 cagaccgagg aagcggatcg cctccagcag gcgcagcgta cccatcgcgt cgacgtcagc
    45301 ggtatattct ggtgactcaa aagagaccgc aacgtggctc attgcgccca ggttgtacac
    45361 ttcatccggc tgtacttcac gcaaaatgcg cgtcaggtta gaggtatcac tcaggtcgcc
    45421 ataatgcaga tggaatttcg ggttgcaggt gtgcggatcc tgataaatgt gatccacgcg
    45481 ctcggtgttg aatgacgatg cgcgacgctt aataccatgc acctcgtaac ctttttccag
    45541 cagaaactct gccaggtaag aaccgtcttg tccggttaca ccggtgatga gagcgactct
    45601 tgacatgtct tattcctctg tatttttgaa tttattcagt ttcaacgcgt tcgcgtatca
    45661 ccactgcggg attcccccgg caaaccacat ttgccggaag cgatttaaaa acactgcttc
    45721 gtgcacccac gacggtgccg tcgccgattg tgacgcctgg ggcaacaaag acatcggttg
    45781 ccagccagca tttctcgcca atcacaatag gcgtggcgtt aatggtgaaa tgttgacttg
    45841 catggtcgtg gctaccggtg cataaataac ttttttgcga tatcaccgaa tgtgcgccaa
    45901 tggttatttc accgagggta tataaattga cgtcatcgcc gacccacgcg taatcaccta
    45961 aggttaattt ccacggatag gtaattttta ctgacggacg aataactacg ttttttccta
    46021 tttttgctcc gaataaacgt aataaaaaag cccgccagcg atacaatact tgtggcgacc
    46081 aggcaaatat tgttgcctgt actgcccacc ataattgcac tttaatagcg ttgccgcccc
    46141 ggaacccttt cggcaccgag aaaccgctta aatcttgcat tggttttcct tatattcaga
    46201 ctttgttata taaggctttc gtctttgagg tagtacgttg gcgtaaatgc caggataatt
    46261 cagcccaaaa gccaggcaca tgtaatattt gtcgttggac ttttttcgcg tcagcacaca
    46321 attccatatt attggtggta gataccccac ccatggaaaa ttcagacacc aggccattga
    46381 gttttttaaa tgcataaccg gctttataca ttttggctgc cagcgcgtag tcggaagaaa
    46441 ctttatattc caggtcataa cgccattttt tcaagccgga tacagggaaa aatatcgcct
    46501 gatgactggc gggcaggctg tgataaatat accagcccgg tttggcgcta cgtttaattt
    46561 tatgcccgtc gccaaaatcc agcagcgcat cgccggtgat catcatgtta tctttttgca
    46621 tttttaactt acggacaaaa tttgcggcat cctgatgaaa aatatcgccc gaattgagaa
    46681 acaacgcgaa cttgccttgt gccatcgtaa tgcctttgtt catggcgtcg tagataccgt
    46741 tatctggctc gctgacaaag cgtaggttat agataccatt gagattttcc agatactcac
    46801 gggtgccgtc attggaaccg ccatcgacaa caatccattc gaagctgata tcttccgcct
    46861 gcgccagatg cgccagcgag gcatgtgttt tgactatccc ttcgaggtta cgaaacgcga
    46921 cagtgattat gctaagcaac atgttcttat tcttacctcg taatatttaa cgcttttcgc
    46981 aaaataaacg gacagacgat taaaaatgca tattccgggc taaatatcga accggtaaaa
    47041 aacagcgata ccggagtaaa aagatataat tgcacgcgaa aattgcggtt atcaccaaaa
    47101 gcgttgatca tcatttttat cactttcccc atgtaccaca gcgataaaaa cactgcgaac
    47161 caggaaaaat aaataatcag cagatacaaa ccattgtcta tggtttttcc gacatccgca
    47221 ccgttaaata ttccgaatga tgcgacgtat tcataaagtg agccaaatct gactacaccg
    47281 tcaatatggg tcaaagaata accgaccatc accagcggac cgacaatacg ataatacgat
    47341 gacgatcctt ccgtacctaa atcgcccaga cgggtggaaa tataaggaaa cgcgattacc
    47401 acaccaacca ggaatacagc cagagaaatc aatgccaacg gtaacttttt cttaatcgcc
    47461 tctttattca gatattgaaa tgcccactcc agcaaataaa acaggataaa agtcataacc
    47521 cctgaaaacg atccggataa tattatccct gcgagaatca tagcatcggt tttaggcgtt
    47581 ttgataccaa actgtttgat gctgagccaa attgagatta atgccagagc gaaaaatgcc
    47641 ggttcgaaat aaagtgctgt cgtgcgcttg ccgccgaatt taatgaaatt cagtacatag
    47701 ctgttgctgt aaatcagata tttcgaaatc gactccataa tactgctgcc accggtgagg
    47761 ataatttgcg ccatctccac cgccgccagc gccaccacca gcccgatcac cagataaaag
    47821 aaacgtaata tcttgcgatg gttgtgtggc gaaatcgttt taaagcgaat actccacacc
    47881 atgccaataa tgatcacaat atagacaaac agcatggttg aagtgacgta tttactggca
    47941 tccagcgact gaccaaacag atagttaaac gccgtgagtc ccgcaccaat ccctaaggca
    48001 atcatcaatt ttttaacgct gatgcgttct aaaaacagca gtaacaagac aggtaaaaaa
    48061 gtgacgatgg tgatggggaa actttcgcca agctgggcga ttttgacgtt aaccagcagg
    48121 tagatcagcg gcagcagcag gtagctacag attctgatag aagttgacat actcctccag
    48181 catctgttgt ccactgtagg tggcgcggct gcgttggctg aactcagcca gcgtggtacc
    48241 aaatatcgcc tgcgcgattt ccggtttgct taactgcacc agttgcagca cgtcttcttc
    48301 gtcgacggtt ttaccgccgg atttttgcaa cacttcccgt gccgcgtcgc tatgggtggc
    48361 aatcaccggc acgccaattg ataacgcctc acacaaaatc agcgggtagt tatcgacgcg
    48421 agaactgaat accagcgcat ccatctgatt gagcgcgctc atcaacttgc gcttgtcagt
    48481 ttcaaagccg tgattaacca cgttgccagc ggtgaacggc gagaacttac caaaggtatg
    48541 cagttcgatt ttgtcgccca gcgccatcat ctcacgcacc agttgctggt tagtcttgcc
    48601 gtcgtaacgc aagtcatgcg ccaccaccgc gattttcggc ttgccctggg tttcgcgcac
    48661 cggaggcaag tccgccagaa tcgcttcggt tgccatatca ataccattat tgataatccg
    48721 gcaacgccct ggaccgtaca ggctattgaa agcgtcagcc acatgctggc tgggggaaat
    48781 aaactgacag cccagcgcca gcatctcacg gaataactgg cgtttgcccg ccaccagctg
    48841 gtgtgcgcga tcaatcttca ccggcggata gttatttaag gtcgggcatt tctggcagcc
    48901 cgttttccag ccttcgcaac cgtcggtaaa ggcgcagcgt ccggtaacac tccagtggtc
    48961 gtgcagcgtc cagaccagag tgacgtccgg tttatggttt ttcacttttt cgcaaaagcg
    49021 caccacgctc ttaagattta gccagtagct gtgcagcaca tgaaaatgca ggaccaccgg
    49081 acccggtgtg cgagtaatgg tgcgatataa ctcattgaaa ttgccaaaca gatcgcgatt
    49141 aaacagacga aacagggcaa tattcgccat cgcggtcatc cgcggcgtat gtttgatgac
    49201 ctgcggatag ttctgatggc tgacgctctc tttgccgcct ttgccgtaac cgtagacaaa
    49261 atgtgacgcc agcccctgtt gcagcgcacg ctggtggaga tctaacgcca cacctgctgc
    49321 cccgccttcc gccagtcgca cattaaattg cagaatattc atttaattac cttcactcgc
    49381 gctttttctc ccaccaccag cgcgttgtcc gggacagagt cgagcaccac gctgcccgca
    49441 cccacggtga cgttgttacc aagcgtgata tcaccaagaa taatgacgtt ggcaccgagt
    49501 tcgacgccgt tgccaatgtg tggacatgcc atgttatcgg caccacgatt gccgatagtg
    49561 acgccgtggc gaatggtgaa atcatccccc gctaccacgt ttttattgat cactacggcg
    49621 taaccgtgat ggatagtaaa gcggcggcca atggtcgcgg cggcctggat ttcataaccg
    49681 aaaaagcatt cggtgataat gcgatacagc accagcagcg gggccgccca cagattgttg
    49741 aggacgttct ttttgcgcca caccgaacaa aaatgagcaa cacgataggc aagaaccatg
    49801 cagcacgggc gtaaactcca gctgttggca cgcagatctt ccagcatctt tagcgccccc
    49861 gaataccatc agccagacgt ttgccgttac gcaccgacag cagcgtcagc aaagtgcgcc
    49921 aggtcatacg tttattgcgg atctggtaga gggtaaacag ctgatatttt ttgcttgcac
    49981 ggtcaaattt atctttgtgc tttcggtaaa agtggaagta cccggagaat tttttcggtg
    50041 acgaggtgat ctgcatttca ccgtgattga tatgcaggat ctgcgtcgct tcttccactt
    50101 tccacggttc gccgtactcc accaccatcc gcaggaagat gtcgtaatcc tgtgctgctt
    50161 tcagttcggt atcgaacaaa cactctttga aacgccatgc ccaagtaaag acctgattgc
    50221 caatgatatt gcgtttatag aacaggcggc gtgagtacgg cgatttggga tacagtggta
    50281 agcttgccgg ttgggaataa acttctccct ggcatacgta gtcgttagcg tacaaaaagg
    50341 cgtgagtgac cagttgctgt ttatgggcga ggaagacgct cagacggttg ggtgtccatt
    50401 cgtcatcgtc atcgatcccg gtaatgtatt ccccttgcgc cagcataatc gcctggttac
    50461 gtaccgcgca cgccccgctg ttaatgtcgt tgtgaatgta ggtgatacgc ggatcgttga
    50521 gggcagtgac gtactgttgc agttgttccc aggaggtgga gcaatcatcc acgatgatca
    50581 tctcccagtt gctgtagtcc tggcgcagga ccgattttat cgcccgaatc gccagttgtt
    50641 ggcggttcca ggtcggcata tagattgaaa tcagcggatt ctctttcata ggttgttctc
    50701 ccggcttatg agctggtttg cttgccggat gcggcgtgag cgccttatcc ggcctacggg
    50761 gcggtgcgaa tgcaggcgtc gttatttcgc atccgactta tattcgtatt cgtaatagcc
    50821 ataatcctga tacgcgctgg cgcggcggaa gatggagttc agaatcaccc ctttcaccgg
    50881 aataccgttt tgctcaaagc ggctcagact ggtttccact tctttcaatg tgttgaccgc
    50941 atagcgcgcc accattaacg tggttccagc gtgacgcccg acaattgctg catcggtcac
    51001 tgccagaatc ggcggcgtat caatcaacac caggtcgtag tttttactcg cccagctcac
    51061 caattcggca aagcgttcgc tcatcaacag ttcagaagga tttggcggca cctgaccgcg
    51121 cgggatcagg tcaaatttgg caatagaggt cggtttagct gcggtagtaa tatcgccctg
    51181 gccaatcaga atttccgaca ggccattgac gttattggta cccaacagct cgtgggtgta
    51241 gcctttgcgc atatcgcagt cgatcaacaa cacccgttta ttggtctgac tgatcaccgc
    51301 cgccaggttg gcgcagacaa aggttttacc gattgacggg ctcacccctg tcatcatcaa
    51361 cacattgttc tgcgcctgca tcatcgcgaa gtgcagactg gtacgcagac tgcggatggc
    51421 ttcaatcgcc agatcggttg gattccccac cgccagtagc tggctctgtt tatagcgttt
    51481 aatccctttg atggttttga cgctatcgcg cgctttctgc cattccgaca gcgggatgct
    51541 ggcatagacg ctgataccat gttcttccag cacctgcggg ctttcgatgc cgcgattaaa
    51601 caacgagcgc agcagcacac ccacgataga gagcatcagg ccgaggataa ttgcgccgag
    51661 gataatcagt cctttcttcg gtttcagcac gccaggctgg gtgattgccg ggtcaacaat
    51721 gcgcacatcg ccgacggtgc tggcttcggt gattttcagc tcctgctctt tattcagcag
    51781 ttgcatatag acctgctgac cagactcgac atcgcgggtc aggcgcacaa tctcctgctg
    51841 ggttttcggc atcgccgtta cgcgaccgtt aagtttggct ttctcttctt ccagcgcctg
    51901 acgtttctcc agcagcgtgc ggtacgccgg gtgaactttg gtgtacagct tggagatttc
    51961 cgcctcttta aaggtcagtt cgttcaactg cgcgtcgatg ttcaccatcg aatcgagcac
    52021 cgctttcgct tccagcggca gatcaacaga atctttatcc tgacggaagg cattcagttt
    52081 gttttcggca acatcgaggc ggctacgtac ttccggtaac tgttgcgcga ggaaagcgag
    52141 gcttttcgac gcttccgccg atttgcgctc aatattttgt tcctgatagt tacgggcgat
    52201 gctgttaaga atgtcgcgga tctgttcgcg atcttcaccg gtataagtca ggctcagtac
    52261 gcctgcgtct ttgccgttct ccgttaccgt caggctgttt tgcagttggt tgatcatccc
    52321 caacgtggag tatttggtga cggtaaactc actgcccggg cgggcgtgaa tggcttcaac
    52381 catcagcgtg acgccttctt ttttcagcat ctggcccgct tgcccacggg cgctaaagcc
    52441 gccatcactg ctcagggtgt agtttttatt gtccagcaca ttaagcgtaa acacctggcc
    52501 cgccatctct ttcgggcggt taaaggtagt cactttcacc gtctcgttct ggcgtcccat
    52561 cagtcgctcc cagcccgccc cgaaaatcgg gaacgtgttt ttgctcactg caatatcgag
    52621 gtcgagatcg tccaccgttt taccaagcac cagacgcgaa cgaatcaact ggatctcggc
    52681 gtccgatgca ggcggtttgt tggctaatgc cgaaccaatg tcctgcacta acgaattgcc
    52741 gctgttttgc tcgatttgta ccagtgcgtc ggcactataa atcggcgttg cgaagaaggt
    52801 gtaaaccacg gcacagaggg caaatacagc ggtgatgcca atcacccacc agcgcgcttc
    52861 aatgacggtg ccgaccaggc gaccaatatc gatttcatca ctgcccgtta ccggagcggc
    52921 atgttgtttt actttttctg tcattcttat acctgctctg cgttcaatgc ctgcgcccac
    52981 tggcgggcag accgttcaag taatgtgtac accgctgcaa acgtttcccg gcttttgcga
    53041 tacggatcgg ggatttcaca ttcgttatcc cagtgaccaa acagcatcac tttgccgcgc
    53101 atctcaggtg ccatctcgca taagcgttcg atatggcgct tttccatggt caaaatcagg
    53161 tcatagttgc gacacaggcg gcggctgatt tgtcgtgcac agtggccttc cagagacagt
    53221 tgatgttctg cggcgacgct gatagcggta ggatcagcgc ccttaccgac cagcgcgccg
    53281 agtccagcgg actccacttt cagttccggg tgataacgtt gcagtaagcg ttccgccgtc
    53341 ggggaacggc aaatattgcc gacacagaca actaatatgt tgttaaacat gacgattacc
    53401 agttatgaat gtcgctggct gtatccgtca tgtaacggac accgctaata gttggcagca
    53461 actgattgat cagacggttc cagcgggaaa ccggggcggt ggtgacatac accacgtcat
    53521 aaggttgcag gcggaattct gtcgccatca ccagcgacgt ggcatcggac atatccagct
    53581 ggtagatatt ggcaatcttg ccattacgcc cgccctcgcc tttcagcgga cgaatgacaa
    53641 agatgccgct ggcgttggag gtggtcatgt cgataccttc agcattaccc agggcttcag
    53701 tcagggtcat gccgctaaag tccattttca gggtgctctg tttcttcact tcacccatca
    53761 caaatacttt cagatcatca ttacgcggca cgtagagaat atcgccggga taaagcagtc
    53821 ggttctgatt gaggtcgccg ttttgcatca gcgcctgcaa agaaatgcgt tcttcacgac
    53881 cattgtgtgt tagcactacg ttgcgccagt cagcggtgtc ggtcaggcca cctgcggcgt
    53941 tgatggcgtc gagaatagtc agtggcacgt tggtgatcgc ctgttgaccg gatttattca
    54001 cctgaccgga gatataggcc ttttgtgagc ggaaggcggc gatattaacg tccacctgcg
    54061 ggtcagcgat gtacgtcgct aagcgcccgg taatatcact gcggatttca gcgagcgttt
    54121 tcccgactac gtgaaccttg ccgatatacg ggtaaaacat agtgccgtca ggctgtaccc
    54181 agttgccggt gtcgctggag ctgcggtact gaccggctgg cgtggtgagt tccgggtgat
    54241 cccagacggt gacattcaga acgtcgcccg gcccgacgcg atactggtaa ttcgcgatct
    54301 cgctttccag cgtcatattg gggcgcgcta cattcgggcg tgggcgtaat tggtcaatca
    54361 ggcgcggggt cagcggataa acattcacca ttttgtcgag atcgaaatca gcgtcctgct
    54421 gtttgatgac gtctttgccc atcgtcgaca tattgctgcc cggaagtact gtgcaaccgc
    54481 ttatcaaggt tactgacacc aataatggca tcaatttcat tttggatttc atcattgttt
    54541 atttatcact ttggcagagt aattatcctg tgcactatta atagcaatat cgccatgcac
    54601 atttaccttg cagttaattg aataaaaatt taactggcat cagtcctaaa aaaattgatt
    54661 tcatccgcag gctattgaca gaataattca gactggtctt tcaggcatcc agacacgcta
    54721 ccgcccctgg ctttttagcc accaatacac tgatttagct taatttttca aaccctctct
    54781 gcatacaatc gttgatgaga aagggttatt gcggaaatta acttccgaat ataaggtgac
    54841 attatggtaa ttgaatattg gctctccaat aatgcaggag gaagtgttac agctaacgga
    54901 atagcaggca aggtaacaat tcggcaattg gctattttta agaattatat taagtgtcat
    54961 tcaatatggt ttttaggagt ttctttaggt tgacaatatt taatattgcg cctccacatg
    55021 cgatatttct taaataatgt tttattatta ccacttttaa ttcagggata atgtgaggtt
    55081 attaccctca aataatatga gataaatagt gctgcaacat tgcatttttg ccccgattta
    55141 tccatgatcg aattgtgaca tttgtcatac aacgaatagg ttttgtactt actatggaat
    55201 ggattgccga tccgtctatc tgggccgggt taatcacgct gattgtgatc gaactggtcc
    55261 tcggcattga taacctggtc tttattgcca tcctcgccga aaaactaccg ccgaggcagc
    55321 gcgaccgcgc acgggttacc gggctgctgc tggcgatgtt aatgcgcctg ttactgctgg
    55381 cgtcaatctc ctggctggtc accctgactc aaccgctgtt cagcttccgc tcgtttacct
    55441 ttagcgcccg cgacttaatc atgctgtttg gtggtttctt cctgttgttc aaagccacga
    55501 tggagctgaa cgaacggctg gaagggaaag acagcaataa tcccacacaa cgcaaaggtg
    55561 cgaagttctg gggcgtggtg acgcaaattg tggtactgga cgccattttc tcactcgact
    55621 cagtgattac cgccgtcggg atggtcgacc atttactggt catgatggcc gccgtggtta
    55681 tcgctatcag cctgatgttg atggccagca agccgttaac gcaatttgtt aacagtcacc
    55741 cgacgatcgt tattctctgc ttaagcttcc tgttgatgat tggctttagc ctggtggcag
    55801 aaggtttcgg cttcgtcatt ccgaaaggct acctgtacgc tgccattggt ttctcggtga
    55861 tgatcgaggc gctcaatcag ttggctatct ttaaccggcg acgttttctt tccgctaacc
    55921 agacgctgcg ccagcggact accgaagcgg taatgcgcct gcttagcggg caaaaagaag
    55981 atgcggaact ggacgccgaa accgcgtcca tgctggtgga tcatggtaac cagcagatct
    56041 ttaatccgca ggaacggcgg atgattgagc gggtacttaa tcttaaccag cgtaccgtca
    56101 gcagcattat gacgtcgcgc cacgatattg agcatatcga tctcaacgcg ccggaagaag
    56161 aaatccgcca gttgctggag cgaaatcagc atacgcggct ggtggttacc gacggtgatg
    56221 acgcagaaga tttgctcggt gttgttcacg ttatcgacct gctacaacag tctctgcgcg
    56281 gcgaaccgct caacctgcgg gtgttgattc gccagccgct ggtgttcccg gaaaccttgc
    56341 cgttgttacc tgccctggag cagttccgta atgcccgcac gcactttgct tttgtggtgg
    56401 atgagtttgg ctcggtggaa gggattgtga cattaagtga cgtcactgaa accattgtcg
    56461 gtaacttacc gaacgaagtg gaagagatcg acgcccgcca tgatattcag aagaatgcgg
    56521 acggttcctg gacggcgaat ggtcatatgc cgctggaaga tctggtgcaa tatgtgccgc
    56581 tgccgctgga tgaaaaacgt gaatatcaca ccattgccgg gctgttgatg gaatatttac
    56641 agcgtattcc aaaacctggc gaagaagttc aggtggggga ttatttgctt aaaacgttgc
    56701 aggtggaaag ccatcgcgtg cagaaggtgc agattattcc gctgcataag gatggcgagt
    56761 tggagtacga ggtgtgatgg tatcggtcat tttacccaat agccgaaagg aataagatga
    56821 gggaaccgcg ccagcggcga tttaccgccg ggagcccggg ttgccagggt ggtggcgatt
    56881 gagccaccct ggcacgttca ccggcttgag cgttccatag tagcgaggaa catcaagtga
    56941 acggaacagc cttcgatctt acatattcag aatataatga tgccgccacg aacgcctgat
    57001 gcgacgcttt acgcgtctta tcaggcctac aggactggtc acctggtaac ctacatcttc
    57061 tccagcaact tcttcacatc cttgccattg cgggaatctt tattccgctc ggcccagtca
    57121 ttcaggcgac gtttcgcttc gtcctgtaga tgtttgcgca gtaactgatc cacttgcagg
    57181 ctgtaattga gttgctgcca gttgccataa acccgcagcg gtactggcgt ttctttcagg
    57241 aaatcaatca gtttactttc cccgttccag ccacccacga cccgaatatc aaactgggta
    57301 tcgcaggttt gatctgccag attcaacatg ccttcccctg tcagcgccag cacaggcgat
    57361 tgaccttgca tgtcgtttaa cgtcacgacg ccatccttca acgtcaaatc ggtggtaaag
    57421 cggtcaagcc gcgtcacgtt atcaaagttt tcagcggcct tcacatcacc gccattacgc
    57481 tctaccgctt gctgaatcat ctgctggaag ttcatccctt ccatgcgcgt gtcggtcatt
    57541 tcgacatgtg cctgtccttg ccagttgtgg cggaatgcgt cggcatctat gtcagcaccg
    57601 gagaaatcac cagccagtga catttttccg gtcaacgaaa tcggatagtt aaacgccttc
    57661 agaatggtac caatctcaac gttttccagc cgtggctgga agtttatccg cggatttatt
    57721 gatgtcgcgt ccagcgtgcc cggcagtgaa acctgtccac cgttaagttt gccctgcagt
    57781 tgagtaattt ccagcaaacc cgacttgttg gtcatttgcg tggcaacatc tgtaaaattc
    57841 attccgcgcc agcgcacgtt actggcctgc aacaaaatat cagccgtaaa gccttgcagt
    57901 ccctgatagg ccggttcatc aatacgcgaa gaaatgaccg ggcgcggcaa cgttgattgg
    57961 ctctgcccct gctgcgcggc accgttttca ccattcgcgg tttcattaag cgggatgagg
    58021 ttgtcaagat tcagttgcgg gaattgcagt ctcagctgcc attccggttt ctctgtcagc
    58081 gtgacctgtg cttgcccgct cagcgtactg tcattggcgg tcaagctgat ttggttaaat
    58141 gaaaggcgtt tatgtgactc ctgccactgg gcctggaaac tcccctgccc ctgaatacct
    58201 tgttttggta aatcggcacc ctgcaactgc cagttaattt gttcaatagc cgccgttaaa
    58261 tcatgcggat aatcagaagc atccaccgta ccgttaaggg atattgtgag atcgcgctga
    58321 tcgcgattaa cccgcccgga gaactcaaat gagccacgat gttggggatc ttgttccatt
    58381 tgcaggcgga tattgcggat tgtcacctgc tcgtcatctt catgctggaa caccagcacg
    58441 ctgtccgcca ccttaagact ggatatatca aacgaccatc cgcgatcgtc tgacagatcc
    58501 ggcaaggtat tgtcgcgcgg tgcaaccgga gcgtcttcac tgcgcaccgc ttccgtctgc
    58561 ggcgtcagtt ggatcactgc cccttttagc atcacctgct taacgctcag ttgatgactc
    58621 agtagtggta aaagcgccac gtccagacgc atgttgtcgg cgcgaaccag tggctggctt
    58681 gcgccctggg cggtgagaga cattcgcccg gagaggatac taagctgcgg ccagacgtgc
    58741 caacgcagtg gcccgtcgag ctgcaattga taaccgctac gtgcagcaac ttgcttgacc
    58801 atatagtcgc ggaaatcatt cggattcacc agcaacacta acgcagataa cccggccacc
    58861 agcacgacca ggagtatcat cagcgtcgtc agaaatcgtc tcatggtatc ctcaatgggc
    58921 ctgaattagt ctttatcgat tcggctggct accgcgccct gctggttgcg atatttcgca
    58981 tcttcacggc ggttgtaagg tcgcgccgcc gggccggaaa gcggctcaaa gctcagcgca
    59041 ccaattaaca tgcccggacg cagcgccagc ggcagcttac cggagttgta gaactccagc
    59101 acaatgcaac cagaccagcc cggatcgata cggtgcgcgg tgacgtgcac catcagcccc
    59161 agacgcgcca aagaggaacg cccgtccagc cagcccacca gatcggctgg cagcgtcacc
    59221 gactccagcg tcaccgccag cgccagctct cctgggtgaa gatagaacgc ctcgccctcg
    59281 tcgagaacga tctcatcgct catcacgcgg tcaagcgcgg cgctcacttc atctttggga
    59341 ccgctcagat cgataaacgc tgccgtgtga ccacggaagg tacgaaattt attgcccagg
    59401 cgtacatcca ccgtcgcgcc gttaatacgc tccactggcg gacgtgggtt gatcgacaaa
    59461 cggccttcat caagccaggc ttcaatatct cggtcacaca gacgcatggc attttctcct
    59521 ttcgcgcatc actcccttaa cgccaattac gtcaagggca taccaggtta tcactgaacg
    59581 gtacacaatt tatcaagctt attcaaagaa ctgacttatt ttcgctttca atatatcgat
    59641 cgcgatgcgg tttttcccgc cgcgcggcac gataatgtcc gcatattgtt tagaaggctc
    59701 aatgaattgc aggaacatcg ggcgcacggt tttttgatat tgcgccatca cggaatccat
    59761 tgaacgccca cgctcgttaa cgtcacgctt gatgcggcgc atcaggcaga tatccagcgg
    59821 ggtatcaacg aaaatggaga agttaagttc gtcacgcaaa cgcgcatccg tcagcaacaa
    59881 aatgccttcg agaatgatga ccttcttcgg ctcaaccgtc accgtttctt tcatacgcgt
    59941 atgttcaaca tagctgtaaa ccggcaggtt aattgccgag ccgcgtttca acgcttgtaa
    60001 atgctcaagc agcaggctgt gatccatcgc gctgggatgg tcgtagttgg tcttaacgcg
    60061 ttcttccatc gacagatggc tttgatcttt gtaatagcag tcttcgggaa ttacgccgat
    60121 gtgttcatca ccgacttgct cacgtaattc acgataaagg gtactggcaa taagactctt
    60181 gccggaagcc gatgcgccag cgataccgat aatgacgcac tgatgagact gatcagtcat
    60241 atatttagcg acctgattaa cctggatgtt aggaaggggg cgacgaagcg ccaaacgcgg
    60301 caattatagg gatttcatcc gtctgatacc agtcgaatag cgttgccgcg cgctcagagt
    60361 taattgttga caaagaattc ccggtggcaa attacgttga tcagttttat gtaaggtaaa
    60421 aaatgttata cgcagttgcg caaattatcc gcctttacgt cactttatga gcaattcgca
    60481 tataaaatgt aaaacttttg tactagcata aacacagaaa cgaatactgg cgacctggtc
    60541 ttgcggataa agcggtaatg agcaaacaat cacagcatgt attaattgcc ctgccccacc
    60601 cgctgcttca cctggtcagt ttaagtttag tctcgtttat ctttaccctt ttctcgcttg
    60661 agctttcgca gtttggcacc caactcgccc cactgtggtt cccgacgtcc atcatgatgg
    60721 tggcgtttta tcgccatgcc gggcgcatgt ggccgggaat tgcgctgagc tgttcgctgg
    60781 gaaatatcgc cgcatccatc ctgctttttt ccaccagctc gctgaacatg acctggacga
    60841 ccatcaatat tgttgaagcc gtggtcgggg cagtgctgct acgtaaattg ctgccgtggt
    60901 ataacccctt gcaaaatctg gctgactggc tgcgtctggc actcggcagc gccattgttc
    60961 cgcctctgtt ggggggtgtt ctggttgtcc tgctgacgcc cggagacgat cctctcaggg
    61021 catttttgat atgggtactg tcagaatcca tcggcgctct ggcactggtg ccgctgggat
    61081 tgttatttaa accacactat ctgctgcgcc atcgcaaccc acggttgctt tttgagtcgc
    61141 tgctcacgtt agccatcaca ctgacgttaa gctggctttc gatgctgtat ctgccgtggc
    61201 cttttacttt cattattgtg ctgttgatgt ggagcgccgt gcgcctgcca cgaatggaag
    61261 cctttttgat cttccttacc acggtgatga tggtgtcgct gatgatggcc gcggatccct
    61321 ccctgcttgc tacgccgcgt acgtacctga tgagccatat gccgtggcta ccgtttttgc
    61381 tgatcctgct gcccgccaac atcatgacca tggtgatgta tgcctttcgt gcggaacgca
    61441 aacacatttc cgaaagcgaa acccgctttc ggaacgcgat ggaatattcc gctatcggta
    61501 tggcgttagt gggcaccgag ggacaatggc tgcaaaccaa caaagcgctc tgccagtttc
    61561 tcgggtacag tcaggaagag ctgcgcggac tcacctttca gcaactgacc tggccggagg
    61621 atctcaataa agatctccaa caggttgaaa agctgataag cggtgaaata aacacctatt
    61681 caatggaaaa acgctactac aaccgcaatg gcgatgttgt ctgggcgttg cttgccgtct
    61741 cactggtgcg ccacacggat ggcacgccgc tctattttat cgctcagatt gaagacatta
    61801 acgagctaaa acgcaccgaa caggtcaatc agcaactgat ggagcgcatc acgctggcta
    61861 acgaagcggg cgggattggc atctgggagt gggagttgaa gccgaatgtt tttagctggg
    61921 ataagcggat gttcgagctg tatgaaattc ctccacatat caaaccgaac tggcaggtgt
    61981 ggtacgagtg cgtgctgccg gaagatcgcc agcatgccga aaaagtgatt cgtgattcgt
    62041 tgcaatcacg ctcgcccttt aaactggaat ttcgcattac cgtaaaagac ggtattcgcc
    62101 atatccgcgc cctcgccaac cgggtactga ataaagaagg cgaagtcgaa cgtctcctcg
    62161 gcatcaatat ggatatgacc gaagtgaaac agcttaacga ggcattgttt caggaaaaag
    62221 agcgcctgca cattacgctt gattccatcg gtgaagccgt ggtctgtatt gatatggcga
    62281 tgaaaattac ctttatgaat ccagtggcgg agaagatgag cggctggacg caggaagaag
    62341 cgttaggtgt tccgctcctg acggtgttgc atattacttt tggcgacaac ggaccattaa
    62401 tggagaacat ttacagtgcc gacacctcac gttccgcaat cgaacaagat gtggtgttgc
    62461 actgtcggag cggcggcagt tacgacgtgc attacagtat tacgccgtta agtactctgg
    62521 acggcagcaa tattggttcg gttctggtga ttcaggacgt caccgaatca cgcaaaatgc
    62581 tgcgccagct gagctacagc gcctcccatg atgcactgac gcatctcgcc aaccgcgcca
    62641 gttttgagaa acaactgcgt atcctgctgc aaacggtaaa cagtacgcat cagcgacatg
    62701 ccctggtgtt tatcgatctt gatcgcttta aagcggtgaa tgacagcgcc gggcatgcgg
    62761 cgggcgacgc tttgctgcgc gaactggcgt cgttgatgct gagtatgctg cgctccagtg
    62821 acgtgctggc gcggctcggc ggtgatgaat ttggtctgct gctgccagac tgcaatgttg
    62881 aaagcgctcg ttttatcgct acacgtatta tcagtgctgt gaatgactat cactttatat
    62941 gggaaggccg ggtacatcgg gtaggtgcca gcgccgggat taccttgatc gatgacaaca
    63001 atcatcaggc ggcagaagtg atgtcgcagg ctgatatcgc ctgttatgcc tccaaaaatg
    63061 gtggacgggg ccgggtgacg gtttacgaac cacagcaagc tgccgcacat agcgaacggg
    63121 cggcgatgtc gcttgatgaa cagtggcgga tgattaaaga gaatcagttg atgatgatcg
    63181 cccacggtgt cgcttcgccg cggatcccgg aagcgcgtaa tttgtggctg atttcgctta
    63241 agctctggag ttgcgaaggc gaaattattg atgaacaaac atttcgtcgt agcttcagcg
    63301 atccggcact tagccatgct cttgaccgac gggtattcca cgaatttttc cagcaggccg
    63361 ccaaagcggt tgccagtaaa ggcataagca tcgccctccc cctttccgtt gccggtttga
    63421 gtagcgccac gctggtgaat gatctgcttg agcagctgga aaatagccct ctaccaccac
    63481 ggttattaca tctgattatt ccggctgaag cgattttaga tcacgcagaa agcgtgcaaa
    63541 aactgcagct ggcgggatgt cggatagtgc tcagccaggt gggccgcgat ctgcaaatct
    63601 tcaactcgct gaaagcgaat atggcagatt acctgctact tgatggtgag ttatgcgcca
    63661 acgtgcaggg taatttgatg gatgagatgc tgattacgat tattcagggg cacgctcagc
    63721 gactcgggat gaaaaccatc gccgggccag tcgttttacc cttagtgatg gatacgcttt
    63781 ctggcatcgg cgtcgatctg atttatggtg atgtgattgc cgatgcccaa ccgctggatt
    63841 tgctggtgaa tagcagttat ttcgcgatta actgagagag cgtttgccca ttgcctgatg
    63901 cgacgctaac gcgtcttatc atgcctacaa atcgctcatt ccccagaccg gataaggcgc
    63961 tcgcaccgca tccggcgacc aacgtcatgc ttcgtctggt tgccagcctt ccatatacca
    64021 gatatgcaac agcgcataag aacgccaggg cttccagcgc tcggcataac ggcggatttg
    64081 tgccggtgtc attcccggaa atcgccgttt aatcagataa tcatccggca gaaaaacatc
    64141 tttcgcctgc cagccacgca aagcaaaata attcgccgtc cagcgcccga tacccggaaa
    64201 agtttgcagc gttttcatcg cctgctccac atcgcccgga attgtcattg gtaagctgcc
    64261 ctccagcgcc gcatttgcca gatgaatcag tgcctctgcc cgtttcaacg gcatgcctaa
    64321 cgcttttaat gcctgcgggt cggctgctgc cagccgctga ggcgtgggga agcagatata
    64381 ctccggaaaa tcatccagcc gttcgccata aagctgtacc actttggcag tcaattttgc
    64441 cgccatcgcc acgctcacca gttggcctaa aatcgcccgc acaccctgct caaaagcatc
    64501 aatacagccg ggtaaacgca atccgggccg ccccgcgcct aactttccca acgcaccgtt
    64561 aacaatctgc gggttacatt gcagatcaag caggcggctc attttcgcca gacactctgc
    64621 ggcaacaggt tctaaacctg cacttaaatt tatgtgcaga gtatggcggg ctatatccgg
    64681 aatagcagtc actacgccgc gatattcgcc caccgccaga ctacgggcat aataattgtc
    64741 cgcgaccgtt tccacaccgc tcaccgcacg ggcggcgaga aatcccaaca tccacgacca
    64801 gtcatacggc ggctgccagt tcagggtata catcgcatct ccttgttaat ccgctttcag
    64861 cataaacgtt attcagacgt tacgctttgc tttcatattc cggttgtcgc gacagcaaca
    64921 tttcgctaaa gtcacgcccc ttcttcaccg gcatggggat taattcgtgt ttattggttt
    64981 tgattacggt acagcaaact gttcagtggc ggtcatgcgt gacggtaaac cgcaattact
    65041 gaaaatggaa aacgacagca cgctgctgcc ttcaatgctt tgcgcgccaa cgcgtgaatc
    65101 ggtaagcgaa tggctgtacc gccatcatga tgttccggca gacgacgatg aaacgcaggc
    65161 gctgttacgt cgggcgattc gttataaccg cgaagaagat atcgatgtta cggcgaaaag
    65221 cgtgcagttc ggtctttcct cactggcaca gtacattgat gatccagaag aagtgtggtt
    65281 tgtgaaatca ccaaaatcgt tcctcggtgc cagcggctta aaaccgcagc aggtagcgct
    65341 gtttgaggat ctggtctgcg caatgatgtt gcacattcgc cagcaggcgc aggcgcagct
    65401 gccagaagcg attactcagg cggtgattgg tcgcccgatc aacttccagg ggctgggcgg
    65461 cgatgaagca aacgcccagg cgcaagagat tctggaacgc gcggcgaagc gtgccggatt
    65521 cagggacgtg gtattccagt acgagccgat cgcggctggg ctggattacg aagccacctt
    65581 gcaggaagaa aaacgggtgc tggtggtgga tatcggcggt ggtacgactg actgttcatt
    65641 gctgctgatg gggccgcagt ggcgttcgcg tctcgatcgt gaagccagtc tgctgggtca
    65701 cagtggttgt cgtattggcg gtaacgatct ggatatcgcc ctggcgttta aaaacctgat
    65761 gccactgctg ggtatgggtg gcgaaaccga aaaaggcatc gccctgccga tcctgccgtg
    65821 gtggaatgcg gttgccatca acgacgtacc tgcgcagagt gatttctaca gtagtgccaa
    65881 cggtcgtctg cttaacgatc tggtacgcga tgcccgcgaa ccggaaaaag tggccctgtt
    65941 acagaaagtc tggcgtcagc gtttaagcta tcgcctggtg cgtagcgcag aagagtgcaa
    66001 aattgctctt tcaagcgtag cggaaacccg cgcctcactg ccgtttatca gcaatgaact
    66061 ggctacgctg attagccagc gagggctgga aagcgccctc agccagccac tggcgcggat
    66121 tctggaacag gtgcaactgg ccctagataa cgcccaggaa aaaccggacg ttatctacct
    66181 gaccggcggt agcgcccgtt ctccgcagat taaaaaagcg ctggcagaac agttgccggg
    66241 cattccgatt gcaggcggcg atgactttgg ctccgtcacc gccgggctgg cacgctgggc
    66301 ggaagtggtg tttcgttaat tgaaaaatgg ctcagcagcg gcttaaatct gcctgcgcct
    66361 gagccagttt ttctgctgcc tgacgtaacg gttccatcaa cggcatggtc cgtgctggag
    66421 catgaaggcg gaatcgctgc aactctgtcg cgccgactgt caacgccgat tccagccgat
    66481 gtcgtttggc ggtcattttc gccattaccg cttgtctgtc tgctggcgtt atcgcttcat
    66541 tcggcctgaa cacaaaacgg cgttcacagc tcgctttcca gtcgataacc gcctgggtca
    66601 gatggtcgcc aaaccctttc acttgcttaa cgccacgacg ggtaacatct gcggctgttt
    66661 caataccaaa agaccgtaac gccgctttac gcgcagggcc aacgccggga atagaggcaa
    66721 catcaataaa aaatccctcc agaaacttct gcttctgccg ttcccttgca gtgtcatgaa
    66781 gtgctgccag agcgcgtttc tcttcttccg gtaacccgag aatttcgtcc ttcatttttt
    66841 cgagcatcgt ccgtttggcg ataaaacctt ccaggccgcc tacctgctgg atctggcgga
    66901 ctaaatggtc atagtccatt ttcgcacgat taaatgcctc acggcgttgc tgaacttctg
    66961 ctttgtacgc tttgcttgtc agactgccaa taatccagat agccgccagc acaggaacca
    67021 aaataatata acgcggttct gcctgaaggc cgcaaagcag cagcgataat gctgacagtg
    67081 cgatctcaag cagaatgatg tattcgcgcc gtaacaggcc taaaggaagc ggcctgccag
    67141 tcggttggaa atgatcgggt aatggcagtt gcaatgctgg cggtgctact gacgccatca
    67201 ccatcgccca gacttttgcc agcacaaaat ccccgccggt ggtaatgacc tcttcgccga
    67261 gatcaataaa atagataaca ccttgattat ccagcgcaca ccatgggcag tcagccaaat
    67321 gaccgggata aacatgcatt gccgaaacgg tacatttctt taactgttgg cgtagagaat
    67381 ccagtgctga tacccacgct ttagccgtcg gacgcccggt tgccacgcca ctttccgtaa
    67441 acgcctgctg aaacatggct tcaacatcac tcggtaacat cgatagcgga atcgatcgtg
    67501 gcggcggttt taaaccacgt cgctgattat ctgacgcgta ggcataacgg aaatgggtaa
    67561 tatccgtctc cagcgcatta cccgcatcag agataagcgg cacaccagaa taaggatgcc
    67621 ggccaccaaa caagacgtga aaaatcagca acgcaaggcc aaaattatcg tgattttctg
    67681 tgcgttcaaa gccgacaaat gacgacaagg tttgtagctc tggcggcgta aaatgcgaca
    67741 cgccgacttc gcataaatgc agtgtgccat tggcgttaat ctgaaaggag tcgctatcaa
    67801 tcaacaccac tttgctgtcg cgacctacca taaaactgtt ctggtttacg tcacccacga
    67861 cgtgcccgtg ctcgtgaacc gtagcaaaag atgaagcaat attgcgcgca acatagagta
    67921 gaaaatccca cgcacaatga gggtaacgtt gacgacgatg tgccgggcta tagatcatat
    67981 gaatcggttc tttaccagaa acttttggca tcataaaacc gataactttt ccgcctcgtc
    68041 caccgtgaag cgttgcctgc ggccaggcga cataattcaa caattgcgcg tcagctgtcg
    68101 cagccataaa ggcaagtttg tcctgtttta aggcgggtgg cggcgtgtga taaatcttgg
    68161 cgacgctatc gacaaactca ttgatatcat aaaccgcgcc ttcaccgcct ttgcccagtt
    68221 cacggcctgg cgtaacgcac tcaccagtag cagtatataa agtgggtttc atggtaacca
    68281 cagcgccagt gccaacgttt tatcatcatc cgtacgctcg tttaccgccg ggctggacaa
    68341 aaactgcttt aacaattcag gtaataaatc gagttgttcc tgcgttgctg acgccagtcc
    68401 attgaaaaac ggggtaaaga aaggcacatg aggagaatta tccagcatat ttaacgccag
    68461 ccgctgaata ccatccgtaa atgctgcaac tttatgcgca cgcccagtgc tggtgaaagt
    68521 ttccagtctg gaaacggcat cttcgtcggt aatgaagtgc gtcatgttgg catattcacc
    68581 caccatcggc gtgagaggca attgcagacc gtgacccaaa tctaccacca cgccgccatc
    68641 gccaatctgc ataatcaagg tgccgtcagg cgatgaaatc aatcccagaa acgtgcaggc
    68701 aaaatcgcga actgctaatg ctttggcttc tgcctcggca aaaagtcgtt gacgaatagt
    68761 cagtaccata tttgtggcga ggacatcatt aagccccaat tcaccgtcct gtactttttg
    68821 caccataaac gccatcgctt cattgacggc gagcgtcgcg ccttctcctc cctgcgaaac
    68881 gctacctgcg ccatcagcaa ggaacatcac cagtaatggt tgctggtcgt ttaaccagac
    68941 aacctgcatc tggcaggcat cctgacaagg caaatcagca ctaatgtgcg atgttccaac
    69001 agcggatgca taaaccagac gccagctcac actgacgtcc agccttttgg cgcttccagc
    69061 acaacttccg ttcccggcgt ggagcgggaa accgaacgca gcgaactgga taaccagcta
    69121 aacagttcac ggaattgtaa tccctgcagt ggcaaaggct gacgaacgct gatttgtgcc
    69181 agagtcttca tatccgcccc ctgaacaccg atggaaaaga aggcaaactt cttatcttct
    69241 tccccctgaa acactttgtt ggcggcagcc tgccactcat cggttggagc gccatcggtg
    69301 atcaggaaaa tccacgggcg ataatacgaa ataccatttg cgcggtactc acgtttcctc
    69361 tcttcaacca tatccagggc tttggtaatg gcggccccca taggcgtatc cccctgggca
    69421 aacaggatgg gcgggaaaaa gttagcggcg ctggtaaagg gttgttccac atgcaccggg
    69481 ccgaacgtca caatgccgag ttccactctt tttaaagcca gcggatcagc aagcagttca
    69541 tcacgaaagg taaccaatcc ggcgttaagt tcattaatag gtctgccgct catcgagcca
    69601 gaaacatcca gcagcaaaat gcaggggcaa cgtggttccg ggttactggc aaaatcgctg
    69661 gtggcaaacg tgatttgttc gctcataaga aatcctttct tagagaatta tcgaggtccg
    69721 gtatcatacc gattgcaata atgaaataag agcaaacgaa gaaactttca gaattaggcc
    69781 cgtcggcgca gaaaattttg ctattcgcct cgcaagaacg gagagccgtc gattgacact
    69841 aagggcggag tgacataatt tcaggagtga gggttaggga gaggtttccc ccctccccct
    69901 ggtgttctta gtaagcctgg aagctaatca ctaagagtat caccagtatg atgacgtgct
    69961 tcatcataac cctttcctta ttaaaagccc tcttctccgg gagagggttt cccgtttcag
    70021 cgtcccgctg aaatcgtcgg cttacctcct ttcgccctga atgcagtcta tcgctaacgc
    70081 gtgaacaaaa tagtttcatc tgtgattatt gcgtaacgac tcgcaaagaa tatttgctgg
    70141 caggatcgca gactacaaag cctgcggatt gacaatctta tcgtgaaggc ataatttcag
    70201 gagtgagggt agagcggggt ttcccccgcc ctggtagtct tagtaagcgg ggaagcttat
    70261 gactaagagc accacgatga tgagtagctt catcatgacc ctttccttat taaggcccct
    70321 tcctcgggag gggctttccc gtttcagcgt cccgctgaaa tcgtcggctt acctcctttc
    70381 gccctgtatg cagtctatcg ctgacttgcg gataaaatag tttccagtgt gattattgcg
    70441 taacgtctcg caaagaatat ttgctggcag gatcgcagac tgcaaagcct gcagattgac
    70501 aatcttatcg tgaaggcata ctttcaggag tgagggtaga gcggggtttc ccccgccctg
    70561 gtagccttag taagctggcc agctaatgac taagagcacc acgatgatga gtagcttcat
    70621 catgaccctt tccttattta tggccccttc ctcgggaggg gctttcccgt ttcagcgtcc
    70681 cgctgaaatc ggcggcttac ctcctttcgc catgcaagca gtctatcgct aacgcgtaga
    70741 taaaatagtt tcctgtgtta ttactggatg cgtgctcgca aatgtgcccg tcattcagac
    70801 gattcccgac agtgtttcat aattcctcca tttttctccc ttattggctg gctacactag
    70861 tatcattccg cgaaacgttt caggaagaga aactcttaac gatgaaaggc agttataaat
    70921 cccgttgggt aatcgtggtg gttatcgccg ccatcgccgc attctggttc tggcaaggcc
    70981 gcaatgactc ccagagtgca gccccagggg cgacgaaaca agcgcagcaa tcgccagcgg
    71041 gtggtcgccg tggtatgcgt tccggcccat tagccccggt tcaggcggcg accgccgtag
    71101 aacaggcagt tccgcgttac ctcaccgggc ttggcaccat tatcgccgct aatactgtta
    71161 cggtgcgcag ccgcgtggac ggtcaactga tggcgttaca tttccaggaa ggccagcagg
    71221 tcaaagcagg cgatttactg gcagaaattg accccagcca gttcaaagtt gcattagcac
    71281 aaacccaggg ccaactggca aaagataaag ccacgcttgc taacgcccgc cgtgatctgg
    71341 cgcgttatca gcaactggca aaaaccaatc tcgtctcccg tcaggagctg gatgcccaac
    71401 aggcgctggt cagtgaaacc gaaggcacca ttaaggctga tgaagcaagc gtcgccagcg
    71461 cgcagttgca actcgactgg agccgcatca ccgcaccagt cgatggtcgc gttggtctca
    71521 agcaggttga tgttggtaac caaatctcca gtggtgatac caccggaatt gtggtgatca
    71581 cccagacgca tcctatcgat ttagtcttta ccctgccgga aagcgatatc gctactgtag
    71641 tgcaggcgca aaaagccgga aaaccgctgg tggtagaagc ctgggatcgc accaattcga
    71701 agaaattaag tgaaggcacg ctgttaagcc tcgataacca aatcgatgcc acaaccggta
    71761 cgattaaagt gaaagcacgc tttaataatc aggatgatgc gctgtttccc aatcagtttg
    71821 ttaacgcgcg catgttagtc gacaccgaac aaaacgccgt agtgatccca acagccgccc
    71881 tgcaaatggg caatgaaggc cattttgtct gggtgctgaa tagcgaaaac aaggtcagca
    71941 aacatctggt gacgccgggc attcaggaca gtcagaaagt ggtgatccgt gcaggtattt
    72001 ctgcgggcga tcgcgtggtg acagacggca ttgatcgcct gaccgaaggg gcgaaagtgg
    72061 aagtggtgga agcccagagc gccaccactc cggaagagaa agccaccagc cgcgaatacg
    72121 cgaaaaaagg agcacgctcc tgatgcaggt gttacccccg agcagcacag gcggcccgtc
    72181 gcgcctgttt attatgcgtc ctgtcgccac cacgctgctg atggtggcga tcttactcgc
    72241 cgggattatc ggttatcgcg ccctgcccgt ttcggcgctg ccggaagtgg actatccgac
    72301 cattcaggtg gtcacactct acccaggtgc cagcccggat gtcatgacct ctgccgttac
    72361 cgcgccgctc gaacgccagt tcgggcagat gtctggcctg aaacagatgt cgtcgcaaag
    72421 ttccggcggt gcgtcagtta tcactttgca gttccagcta acattaccgc tcgatgtcgc
    72481 cgagcaggaa gtgcaggccg cgattaacgc tgcgaccaac ttgttgccga gcgatctgcc
    72541 taacccgccg gtttacagca aagtgaaccc ggcagatccg ccgatcatga cgctcgccgt
    72601 cacctcaacc gccatgccga tgacgcaagt ggaagatatg gtggaaaccc gcgtcgcgca
    72661 gaaaatctcg cagatttccg gcgtcggcct ggtgacgctt tccggcggtc agcgtccggc
    72721 tgttcgcgtc aaacttaacg ctcaggcgat tgccgccctc ggcctgacca gcgaaaccgt
    72781 gcgcaccgcc attaccggcg ctaacgttaa ctcggcaaaa ggtagcctcg acggcccttc
    72841 ccgtgcggtc acgctttccg cgaacgacca gatgcaatcc gccgaagagt atcgccagct
    72901 aatcatcgcc taccagaacg gcgcgccaat tcgtctgggc gatgtcgcaa ctgtagagca
    72961 aggtgcagaa aacagctggc tcggcgcgtg ggcgaacaaa gaacaggcca ttgtgatgaa
    73021 tgttcagcgc cagcccggtg ctaacattat ctccaccgcc gacagcattc ggcagatgct
    73081 gccacagctc actgagagtc tgccgaaatc ggtgaaggtg acagtgcttt ccgatcgcac
    73141 caccaatatc cgcgcatccg tcgatgatac tcagtttgaa ttgatgatgg ctatcgcgct
    73201 ggtagtcatg attatctacc tgtttttgcg caatattccg gcgaccatca ttcccggtgt
    73261 tgctgtaccg ctgtcgttaa tcggcacttt cgcggttatg gtgtttctcg atttttcaat
    73321 caataacctg acactgatgg cgttaactat cgccaccgga ttcgtggtcg atgacgccat
    73381 cgtggtgatc gaaaacattt cccgctatat cgaaaaaggc gaaaaaccgt tggcggcggc
    73441 gctcaagggc gcaggtgaaa tcggctttac cattatctcg ctgaccttct cactgattgc
    73501 ggtgttgatc ccactgctgt ttatgggcga tatcgtcggg cgactgttcc gcgaatttgc
    73561 tattaccctg gcggtagcga ttttgatctc agcggtggtg tcgctgaccc tgacaccgat
    73621 gatgtgcgcg cggatgctca gccaagagtc gttgcgtaaa cagaaccgct tctcccgtgc
    73681 ctcggaaaaa atgttcgaca ggataatcgc cgcctatggt cgtggactgg cgaaagtgct
    73741 gaatcatccg tggctgacct taagcgtggc actcagcacg ctgctgctta gcgtgctgct
    73801 gtgggtgttc attccgaaag gtttcttccc ggtacaggac aatggcatta ttcagggcac
    73861 tttgcaggca ccgcaatcca gctcctttac caatatggcc cagcgacaac gccaggtcgc
    73921 ggacgtgatt ttgcaggatc cggcagtgca aagcctgacc tcatttgttg gcgttgatgg
    73981 cactaacccg tcgctgaata gtgcacgttt acaaatcaac ctcaaaccgt tggatgaacg
    74041 tgatgatcgg gtgcaaaaag tcatcgcccg tctgcaaacg gcggtagata aagtgccggg
    74101 cgtcgatctc ttcctgcaac caacgcagga tctgactatt gatactcagg tcagccgcac
    74161 ccagtaccag tttaccttgc aggccacctc actggatgcg ctcagtacct gggtgccaca
    74221 gttgatggaa aaactccagc aactgccaca gctttctgat gtctccagcg actggcagga
    74281 ccaagggctg gtggcgtatg tcaatgttga tcgcgacagc gccagccgtc tggggatcag
    74341 catggcggat gtcgataacg ccctgtacaa cgcgtttggt cagcgactga tttccactat
    74401 ttatactcag gctaaccagt atcgcgtagt gctggagcac aacaccgaaa ttaccccggg
    74461 cctcgcggcg ctggatacca ttcgcctgac cagtagcgac ggcggcgtgg tgccgttaag
    74521 ctcaattgcc aaagttgaac agcgttttgc gccgctttcc atcaaccatc tggatcagtt
    74581 cccggtaacg accatctcct ttaacgtgcc ggataactat tcgctgggcg atgcggtgca
    74641 ggagattatg gacaccgaaa agacgctgaa tctgccggtg gatatcacca cgcaattcca
    74701 gggcagcacc ctcgccttcc agtcggcgct gggcagcact gtctggctga ttgtcgcggc
    74761 ggtggtggcg atgtatatcg tgctcggcat tctgtacgag agctttattc acccgatcac
    74821 cattctctcg acgctaccca ccgcaggggt cggcgcgctg ctggcattaa tgattgccgg
    74881 tagcgaactg gatgtgattg cgattatcgg cattattttg ctgatcggta ttgtgaagaa
    74941 gaacgccatc atgatgatcg acttcgcgct ggctgctgag cgcgagcaag gcatgtcgcc
    75001 gcgcgatgca atctaccagg cttgtctgtt gcgttttcgt ccgatcctga tgaccaccct
    75061 ggcggctctg cttggcgcgc tgccgctgat gttgagtacc ggggtcggcg cggaactgcg
    75121 tcgtccgtta ggtatcggca tggtcggcgg tctgattgtc agccaggtgc tgacgctgtt
    75181 caccacgccg gtgatttatc tgctgttcga ccgcctggca ttgtggacca aaagtcgctt
    75241 tgctcgccat gaagaggagg cgtaagtgaa gttttttgcc ctcttcattt accgcccggt
    75301 ggcgacgatt ttactgtcgg tcgccattac cctgtgcggc atactgggct tccgtatgct
    75361 gccggtcgcc ccgctgccgc aggtcgattt tccggtgatt atggtcagcg cctcgctgcc
    75421 cggtgcgtca ccagaaacaa tggcgtcttc cgttgccacg ccgctggagc gctcgcttgg
    75481 gcgcattgcc ggggtcagcg aaatgacctc cagcagttcg ctcggcagca cacgtattat
    75541 tttgcagttt gattttgacc gggacatcaa cggcgcagcg cgtgatgtgc aggcggcgat
    75601 caacgctgca caaagtttgc tgcccagtgg gatgcccagc cgtccgacct atcgcaaagc
    75661 gaacccgtcg gatgcgccaa ttatgatcct cacgctgaca tccgatactt attcgcaggg
    75721 tgaactgtac gatttcgcct cgacgcagct ggctccgacg atttcgcaaa tcgacggtgt
    75781 tggtgatgtc gatgtcggag gcagctcact gcccgccgta cgcgtcgggc tgaatccgca
    75841 ggcgctgttt aatcagggcg tgtcgctgga cgacgtacgc accgccatca gcaatgccaa
    75901 cgtgcgtaaa ccgcagggcg cgctggaaga tggcactcac cgctggcaga tccagaccaa
    75961 tgatgagcta aaaaccgccg ctgaatatca gccgttgatt attcactaca acaacggcgg
    76021 cgcggttcgt ctgggcgatg tggcgacggt gaccgactca gtgcaggatg tgcgcaacgc
    76081 cgggatgacc aacgccaaac cggctatttt actgatgatc cgcaaactgc cggaagccaa
    76141 tattatccag acggttgaca gcatccgggc aaaattaccg gagttgcagg aaaccattcc
    76201 ggcggcgatt gatctgcaaa ttgcccagga tcgctccccc accattcgcg cctcgctgga
    76261 agaagtcgag caaacgctga ttatctcggt ggcgctggtg attctggtgg tgtttttatt
    76321 cctgcgctcg ggtcgcgcca ctattattcc cgccgtttcg gtgccggttt cgctgattgg
    76381 tacgtttgcg gcgatgtacc tgtgcggatt cagtctcaat aacctttcgt taatggcgct
    76441 caccatcgct actggtttcg tggtggatga cgccatcgtg gtgctggaaa acattgcacg
    76501 tcatctggaa gcgggaatga aaccgttgca agccgcactg caaggtactc gcgaagtcgg
    76561 ttttacggtg ctgtcgatga gtctgtcact ggtggcggtg ttcctgccgc tgctgttgat
    76621 gggcggattg ccgggccgac tgttacgcga gtttgccgtg acgctttctg tcgccattgg
    76681 tatttcgttg ctggtttctc tgacattaac gccaatgatg tgtggctgga tgctgaaagc
    76741 cagcaagccg cgcgagcaaa agcgactgcg tggttttggt cgcatgttgg tagccctgca
    76801 acaaggctac ggcaagtcac tgaaatgggt gctcaatcat acccgtctgg tgggcgtggt
    76861 gctgcttggc accattgcgc tgaatatctg gctgtatatc tcgatcccga aaaccttctt
    76921 cccggagcag gacactggcg tgttgatggg cgggattcag gcggatcaga gtatttcgtt
    76981 tcaggcgatg cgcggtaagt tgcaggattt catgaaaatt atccgtgacg atccggcagt
    77041 ggataatgtc accggcttta caggcggttc gcgagtgaac agcgggatga tgtttatcac
    77101 cctcaagcca cgcgacgaac gcagcgaaac ggcgcagcaa attatcgacc gtctgcgcgt
    77161 aaaactggcg aaagaaccgg gggcgaatct gttcctgatg gcggtacagg atattcgcgt
    77221 tggtgggcgt cagtcgaacg ccagctacca gtacacgttg ttatccgacg acctggcggc
    77281 actgcgcgaa tgggagccga aaatccgcaa aaaactggcg acgttgccgg aactggcgga
    77341 cgtgaactcc gatcagcagg ataacggcgc ggagatgaat ctggtttacg accgcgacac
    77401 catggcacgg ctgggaatcg acgtgcaggc cgccaacagc ctgttaaata acgccttcgg
    77461 tcagcggcaa atctcgacca tttaccagcc gatgaaccag tataaagtgg tgatggaagt
    77521 ggatccgcgc tatacccagg acatcagtgc gctggaaaaa atgttcgtta tcaataacga
    77581 aggcaaagcg atcccgctgt cgtatttcgc taaatggcaa ccggcgaacg ctccgctgtc
    77641 ggtgaatcat cagggattat cggcggcctc gaccatctcg ttcaacctgc cgaccggaaa
    77701 atcgctctcg gacgccagtg cggcgatcga tcgcgcaatg acccagcttg gtgtgccttc
    77761 gacggtgcgc ggcagttttg ccggaacagc gcaggtgttc caggagacga tgaactcaca
    77821 ggtgatcctg attatcgccg ccatcgccac ggtgtatatc gtgctgggta tcctttacga
    77881 gagttacgta cacccactga cgattctctc caccctgccc tcggcgggcg ttggtgcgct
    77941 gctggcgctg gagctgttca atgccccgtt cagcctaata gccctgatag ggatcatgct
    78001 attaatcggc atcgtaaaga aaaacgccat tatgatggtc gattttgcgc tcgaagccca
    78061 acggcacggt aacctgacgc cgcaggaagc tattttccag gcctgtctgc tgcgttttcg
    78121 cccgattatg atgactaccc tggcggcgct gtttggcgcg ctgccgctgg tgttgtcggg
    78181 cggtgacggc tcggagctgc ggcaccccct ggggatcacc attgtcggtg gactggtaat
    78241 gagccagctt ctgacgctgt ataccacgcc ggtggtgtat ctctttttcg accgtctgcg
    78301 gctgcgtttt tcgcgtaaac ctaaacaaac ggtaaccgag taaatgacag aacttcccga
    78361 cagcacccgc tggcaattgt ggattgtggc tttcggcttc tttatgcagt cgctggacac
    78421 caccatcgtt aacaccgccc ttccctcaat ggcgcaaagc ctcggggaaa gtccgttgca
    78481 tatgcacatg gtcattgtct cttatgtgct gaccgtggcg gtgatgctgc ccgccagcgg
    78541 ctggctggcg gacaaagtcg gcgtgcgcaa tattttcttt accgccatcg tgctgtttac
    78601 tctcggttca ctgttttgcg cgctttccgg cacgctgaac gaactgttgc tggcacgcgc
    78661 gttacagggc gttggcggtg cgatgatggt gccggtcggc aggttgacgg tgatgaaaat
    78721 cgtaccgcgc gagcaatata tggcggcgat gacctttgtc acgttacccg gtcaggtcgg
    78781 tccgctgctc ggtccggcgc tcggcggtct gctggtggag tacgcgtcgt ggcactggat
    78841 ctttttgatc aacattccgg tggggattat cggtgcgatc gccacattgc tgttaatgcc
    78901 gaactacacc atgcagacgc ggcgctttga tctttccggc tttttattgc tggcggttgg
    78961 catggctgta ttaaccctgg cgctggacgg cagtaaaggt acaggtttat cgccgctggc
    79021 gattgctggc ctggtcgcag ttggcgtggt ggcactggtg ctttatctgc tgcacgccag
    79081 aaataacaac cgcgccctgt tcagtctgaa actgttccgc actcgtacct tttcgctggg
    79141 cctggcgggg agctttgccg gacgtattgg cagtggcatg ttgcccttta tgacaccggt
    79201 tttcctgcaa attggcctcg gtttctcgcc gtttcatgcc ggactgatga tgatcccgat
    79261 ggtacttggc agcatgggaa tgaagcgaat tgtggttcag gtggtgaatc gctttggtta
    79321 tcgtcgggta ctggtagcga ccacgctggg tctgtcgctg atcaccctgt tgtttatgac
    79381 taccgctctg ttgggctggt actacgtttt gccgttcgtc ctgtttttac aagggatggt
    79441 caactcgacc cgtttctcct ccatgaacac cctgacgctg aaagatctcc cggacaatct
    79501 ggcgagcagc ggcaacagtc tgctgtcgat gattatgcaa ttgtcgatga gtatcggcgt
    79561 caccatcgcc gggctgttgc tgggactttt tggttcgcag catgtcagcg tcgacagcgg
    79621 caccacacaa accgtcttta tgtacacctg gcttagcatg gcgtttatca tcgcccttcc
    79681 ggcgttcatc tttgccagag tgccgaacga tacgcatcaa aatgtagcta tttcgcggcg
    79741 aaaaaggagc gcgcaatgaa gttctggcga cctggtatta ccggcaaact gtttctggcg
    79801 attttcgcca cctgcattgt tttgctgatc agtatgcact gggcggtgcg tatcagtttt
    79861 gagcgtggct ttattgatta catcaagcat ggtaatgaac agcgattaca actgttaagt
    79921 gatgcgcttg gcgagcagta tgcgcagcat ggcaactggc gcttcctgcg caacaatgat
    79981 cgctttgtct ttcagatcct gcgttcattt gaacacgata attcggaaga taaacccggc
    80041 ccgggtatgc caccgcacgg ctggcgtacc cagttctggg tggttgatca aaacaacaaa
    80101 gtgctggttg gtccgcgagc gccgattcca cctgacggta cacggcgacc cattctggtc
    80161 aacggtgcgg aagttggcgc ggtgatcgcc tcccccgttg agcggttaac gcgcaatact
    80221 gatatcaatt tcgataaaca acagcggcaa accagctggt tgattgtcgc cctggcaacg
    80281 ttactcgcgg cacttgccac ttttctgctg gcgcgcggtt tactggcacc ggtaaaacga
    80341 cttgtcgatg gcacgcacaa actggcggcg ggcgatttca ctacccgcgt aacgcccacc
    80401 agtgaagatg aactgggcaa actggcgcaa gacttcaacc agcttgccag cacactggag
    80461 aaaaaccagc aaatgcggcg cgattttatg gccgatattt ctcacgaact gcgtacgcca
    80521 ttagcggtgc tgcgcggtga actggaagct attcaggatg gcgtgcgcaa attcacgccg
    80581 gagacggtgg cgtctttaca ggcggaggtc ggtacactga ccaaactggt tgacgatctc
    80641 catcagttgt cgatgtctga tgaaggcgcc ctcgcctatc aaaaagcacc ggtagatttg
    80701 atcccactgc tggaagtggc gggcggcgca tttcgcgaac gattcgccag ccgtggcctg
    80761 aaactgcaat tttccctgcc agacagtatt accgtatttg gcgatcgcga ccgtttaatg
    80821 cagttattca ataatttact tgaaaacagc ctgcgctaca ctgacagcgg cggcagcctg
    80881 aaaatctctg ccgagcagca cgacaaaacg gtgcgcctga cctttgccga cagtgcgcca
    80941 ggtgtcagtg acgagcagct acaaaaatta tttgaacgtt tttatcgcac cgaaggttcc
    81001 cgcaaccgcg ccagcggtgg ttccgggctg gggctggcga tttgcctgaa cattgttgaa
    81061 gcacataatg gccgcattat tgctgcccat tcgccttttg gcggggtaag cattacagta
    81121 gagttaccgc tggaacggga tttacagaga gaagtatgac cgagttacca atcgacgaaa
    81181 acacaccgcg tattttgatc gtggaagatg aaccgaagct ggggcagttg ctcattgatt
    81241 atctgcgtgc tgcgagctat gcgccgacgc ttatcagcca cggcgatcag gtactgccgt
    81301 atgtgcgcca gacaccaccg gatctgatcc tgttggatct gatgctccct ggcaccgatg
    81361 gcttgacgct gtgccgggaa attcgtcgtt tttctgacat tcctatcgtg atggtgacgg
    81421 caaaaatcga agagatcgac cgcctgctcg gactggagat tggcgcagat gactacatct
    81481 gcaagccgta cagcccacgg gaagtggtag cgcgcgtcaa aaccattttg cgccgttgca
    81541 aaccgcagcg cgaattgcag caacaggatg ccgaaagccc gttgattatc gacgaaggtc
    81601 gttttcaggc ttcatggcgc ggtaaaatgc ttgacctgac gcctgcggaa tttcgtctgt
    81661 tgaaaacgct ctctcatgaa ccaggaaaag tgttctcccg cgagcaattg ctcaatcatc
    81721 tttatgacga ctaccgcgta gtaaccgacc gcaccattga cagccacatt aaaaacctgc
    81781 gccgcaagct ggaatctctc gacgccgaac agtcatttat ccgcgccgtt tacggcgttg
    81841 gttaccgctg ggaagccgac gcctgccgca tcgtttagtt ttagcgacat gactttgtta
    81901 gccggagatg gcgttcagac cagatccggc aacattatcc cacgcatggt cagcaaactg
//
LOCUS       DAKISA010000004        78723 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.004, whole genome shotgun sequence.
ACCESSION   DAKISA010000004 DAKISA010000000
VERSION     DAKISA010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 78723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78723
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.004"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..343
                     /gene="bluF"
                     /locus_tag="OVW45_000243"
     CDS             <1..343
                     /gene="bluF"
                     /locus_tag="OVW45_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HCV5524788.1"
                     /translation="AIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
     gene            548..1279
                     /gene="bluR"
                     /locus_tag="OVW45_000244"
     CDS             548..1279
                     /gene="bluR"
                     /locus_tag="OVW45_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HCV5524789.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWNRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            1500..1904
                     /locus_tag="OVW45_000245"
     CDS             1500..1904
                     /locus_tag="OVW45_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HCV5524790.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIYGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            1957..2067
                     /locus_tag="OVW45_000246"
     CDS             1957..2067
                     /locus_tag="OVW45_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5524791.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHQANTIKLIFEM"
     gene            2303..2347
                     /locus_tag="OVW45_000247"
     CDS             2303..2347
                     /locus_tag="OVW45_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5524792.1"
                     /translation="MFSKLAPSSIKAMF"
     gene            2600..2923
                     /gene="iraM"
                     /locus_tag="OVW45_000248"
     CDS             2600..2923
                     /gene="iraM"
                     /locus_tag="OVW45_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCV5524793.1"
                     /translation="MKWVVIDTVIQPTCGISFSAIWGDMKMIIWYQSTIFLPPGSIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESKITNKCLHNSCIIKIC
                     PYGLK"
     gene            complement(3026..4276)
                     /gene="icd"
                     /locus_tag="OVW45_000249"
     CDS             complement(3026..4276)
                     /gene="icd"
                     /locus_tag="OVW45_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCV5524794.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            4480..5100
                     /gene="rluE"
                     /locus_tag="OVW45_000250"
                     /pseudo
     CDS             4480..5100
                     /gene="rluE"
                     /locus_tag="OVW45_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873386.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
     gene            5110..5571
                     /gene="nudJ"
                     /locus_tag="OVW45_000251"
     CDS             5110..5571
                     /gene="nudJ"
                     /locus_tag="OVW45_000251"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCV5524795.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            5625..6731
                     /gene="mnmA"
                     /locus_tag="OVW45_000252"
     CDS             5625..6731
                     /gene="mnmA"
                     /locus_tag="OVW45_000252"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCV5524796.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPGQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            6767..7408
                     /gene="hflD"
                     /locus_tag="OVW45_000253"
     CDS             6767..7408
                     /gene="hflD"
                     /locus_tag="OVW45_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCV5524797.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            7412..8782
                     /gene="purB"
                     /locus_tag="OVW45_000254"
     CDS             7412..8782
                     /gene="purB"
                     /locus_tag="OVW45_000254"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCV5524798.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            8950..9621
                     /gene="phoP"
                     /locus_tag="OVW45_000255"
     CDS             8950..9621
                     /gene="phoP"
                     /locus_tag="OVW45_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCV5524799.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            9621..11081
                     /gene="phoQ"
                     /locus_tag="OVW45_000256"
     CDS             9621..11081
                     /gene="phoQ"
                     /locus_tag="OVW45_000256"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCV5524800.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            11157..12278
                     /gene="roxA"
                     /locus_tag="OVW45_000257"
                     /gene_synonym="ycfD"
     CDS             11157..12278
                     /gene="roxA"
                     /locus_tag="OVW45_000257"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCV5524801.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(12327..13553)
                     /gene="pepT"
                     /locus_tag="OVW45_000258"
     CDS             complement(12327..13553)
                     /gene="pepT"
                     /locus_tag="OVW45_000258"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCV5524802.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            13803..14939
                     /gene="potA"
                     /locus_tag="OVW45_000259"
     CDS             13803..14939
                     /gene="potA"
                     /locus_tag="OVW45_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCV5524803.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            14923..15780
                     /gene="potB"
                     /locus_tag="OVW45_000260"
     CDS             14923..15780
                     /gene="potB"
                     /locus_tag="OVW45_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCV5524804.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            15777..16571
                     /gene="potC"
                     /locus_tag="OVW45_000261"
     CDS             15777..16571
                     /gene="potC"
                     /locus_tag="OVW45_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCV5524805.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            16568..17614
                     /gene="potD"
                     /locus_tag="OVW45_000262"
     CDS             16568..17614
                     /gene="potD"
                     /locus_tag="OVW45_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCV5524806.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(17770..18591)
                     /gene="cobB"
                     /locus_tag="OVW45_000263"
     CDS             complement(17770..18591)
                     /gene="cobB"
                     /locus_tag="OVW45_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCV5524807.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(18607..19517)
                     /gene="nagK"
                     /locus_tag="OVW45_000264"
                     /pseudo
     CDS             complement(18607..19517)
                     /gene="nagK"
                     /locus_tag="OVW45_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
     gene            complement(19546..20790)
                     /gene="lolE"
                     /locus_tag="OVW45_000265"
     CDS             complement(19546..20790)
                     /gene="lolE"
                     /locus_tag="OVW45_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCV5524808.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGTNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(20790..21491)
                     /gene="lolD"
                     /locus_tag="OVW45_000266"
     CDS             complement(20790..21491)
                     /gene="lolD"
                     /locus_tag="OVW45_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCV5524809.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(21484..22683)
                     /gene="lolC"
                     /locus_tag="OVW45_000267"
     CDS             complement(21484..22683)
                     /gene="lolC"
                     /locus_tag="OVW45_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCV5524810.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            22945..24018
                     /locus_tag="OVW45_000268"
     CDS             22945..24018
                     /locus_tag="OVW45_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCV5524811.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            24146..27592
                     /gene="mfd"
                     /locus_tag="OVW45_000269"
     CDS             24146..27592
                     /gene="mfd"
                     /locus_tag="OVW45_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCV5524812.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            27736..28692
                     /gene="ldtC"
                     /locus_tag="OVW45_000270"
                     /pseudo
     CDS             27736..28692
                     /gene="ldtC"
                     /locus_tag="OVW45_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(28775..29032)
                     /gene="bhsA"
                     /locus_tag="OVW45_000271"
     CDS             complement(28775..29032)
                     /gene="bhsA"
                     /locus_tag="OVW45_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HCV5524813.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            29273..29905
                     /gene="comR"
                     /locus_tag="OVW45_000272"
     CDS             29273..29905
                     /gene="comR"
                     /locus_tag="OVW45_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCV5524814.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(29967..30506)
                     /locus_tag="OVW45_000273"
     CDS             complement(29967..30506)
                     /locus_tag="OVW45_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCV5524815.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(30733..32037)
                     /gene="ndh"
                     /locus_tag="OVW45_000274"
     CDS             complement(30733..32037)
                     /gene="ndh"
                     /locus_tag="OVW45_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCV5524816.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(32445..32987)
                     /locus_tag="OVW45_000275"
     CDS             complement(32445..32987)
                     /locus_tag="OVW45_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCV5524817.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(33010..34035)
                     /gene="nagZ"
                     /locus_tag="OVW45_000276"
     CDS             complement(33010..34035)
                     /gene="nagZ"
                     /locus_tag="OVW45_000276"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCV5524818.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(34046..34870)
                     /gene="thiK"
                     /locus_tag="OVW45_000277"
     CDS             complement(34046..34870)
                     /gene="thiK"
                     /locus_tag="OVW45_000277"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCV5524819.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQIGDQQFIRLADDTWRQLLIKQ"
     gene            complement(34851..35492)
                     /gene="lpoB"
                     /locus_tag="OVW45_000278"
     CDS             complement(34851..35492)
                     /gene="lpoB"
                     /locus_tag="OVW45_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCV5524820.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(35506..35883)
                     /locus_tag="OVW45_000279"
     CDS             complement(35506..35883)
                     /locus_tag="OVW45_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCV5524821.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(35886..36245)
                     /gene="hinT"
                     /locus_tag="OVW45_000280"
     CDS             complement(35886..36245)
                     /gene="hinT"
                     /locus_tag="OVW45_000280"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCV5524822.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            36579..38768
                     /gene="fhuE"
                     /locus_tag="OVW45_000281"
     CDS             36579..38768
                     /gene="fhuE"
                     /locus_tag="OVW45_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HCV5524823.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQLDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNMEGSIVYGAPRNFSITGTYQF"
     gene            complement(38828..40261)
                     /gene="ptsG"
                     /locus_tag="OVW45_000282"
     CDS             complement(38828..40261)
                     /gene="ptsG"
                     /locus_tag="OVW45_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCV5524824.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(40556..41353)
                     /locus_tag="OVW45_000283"
     CDS             complement(40556..41353)
                     /locus_tag="OVW45_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCV5524825.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(41364..42368)
                     /gene="holB"
                     /locus_tag="OVW45_000284"
     CDS             complement(41364..42368)
                     /gene="holB"
                     /locus_tag="OVW45_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCV5524826.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(42365..43006)
                     /locus_tag="OVW45_000285"
     CDS             complement(42365..43006)
                     /locus_tag="OVW45_000285"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCV5524827.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(42996..44018)
                     /gene="yceG"
                     /locus_tag="OVW45_000286"
     CDS             complement(42996..44018)
                     /gene="yceG"
                     /locus_tag="OVW45_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCV5524828.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(44021..44830)
                     /gene="pabC"
                     /locus_tag="OVW45_000287"
     CDS             complement(44021..44830)
                     /gene="pabC"
                     /locus_tag="OVW45_000287"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCV5524829.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(44950..46191)
                     /gene="fabF"
                     /locus_tag="OVW45_000288"
     CDS             complement(44950..46191)
                     /gene="fabF"
                     /locus_tag="OVW45_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCV5524830.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(46279..46515)
                     /gene="acpP"
                     /locus_tag="OVW45_000289"
     CDS             complement(46279..46515)
                     /gene="acpP"
                     /locus_tag="OVW45_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCV5524831.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(46726..47460)
                     /gene="fabG"
                     /locus_tag="OVW45_000290"
     CDS             complement(46726..47460)
                     /gene="fabG"
                     /locus_tag="OVW45_000290"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCV5524832.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(47473..48402)
                     /gene="fabD"
                     /locus_tag="OVW45_000291"
     CDS             complement(47473..48402)
                     /gene="fabD"
                     /locus_tag="OVW45_000291"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCV5524833.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKLMQEAVPEGTGAMAAIIGLDDASIAKACEEAAAGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(48418..49371)
                     /gene="fabH"
                     /locus_tag="OVW45_000292"
     CDS             complement(48418..49371)
                     /gene="fabH"
                     /locus_tag="OVW45_000292"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCV5524834.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(49439..50509)
                     /gene="plsX"
                     /locus_tag="OVW45_000293"
     CDS             complement(49439..50509)
                     /gene="plsX"
                     /locus_tag="OVW45_000293"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCV5524835.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(50590..50763)
                     /gene="rpmF"
                     /locus_tag="OVW45_000294"
     CDS             complement(50590..50763)
                     /gene="rpmF"
                     /locus_tag="OVW45_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCV5524836.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(50815..51336)
                     /gene="yceD"
                     /locus_tag="OVW45_000295"
     CDS             complement(50815..51336)
                     /gene="yceD"
                     /locus_tag="OVW45_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCV5524837.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            51535..52119
                     /gene="yceF"
                     /locus_tag="OVW45_000296"
     CDS             51535..52119
                     /gene="yceF"
                     /locus_tag="OVW45_000296"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCV5524838.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(52231..53190)
                     /gene="rluC"
                     /locus_tag="OVW45_000297"
     CDS             complement(52231..53190)
                     /gene="rluC"
                     /locus_tag="OVW45_000297"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCV5524839.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLIAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            53763..56948
                     /gene="rne"
                     /locus_tag="OVW45_000298"
     CDS             53763..56948
                     /gene="rne"
                     /locus_tag="OVW45_000298"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCV5524840.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTGVPVAAAVEPVVSAPVVEEVAEVVEAPVQIAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATVPMTRAPAPEYVPDAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(57143..58096)
                     /gene="flgL"
                     /locus_tag="OVW45_000299"
     CDS             complement(57143..58096)
                     /gene="flgL"
                     /locus_tag="OVW45_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HCV5524841.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(58108..59751)
                     /gene="flgK"
                     /locus_tag="OVW45_000300"
     CDS             complement(58108..59751)
                     /gene="flgK"
                     /locus_tag="OVW45_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HCV5524842.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITVANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQELDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFTIGKPA
                     VLQNTKNKGDVAIGATVTDASVVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(59817..60758)
                     /gene="flgJ"
                     /locus_tag="OVW45_000301"
     CDS             complement(59817..60758)
                     /gene="flgJ"
                     /locus_tag="OVW45_000301"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCV5524843.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(60758..61855)
                     /gene="flgI"
                     /locus_tag="OVW45_000302"
     CDS             complement(60758..61855)
                     /gene="flgI"
                     /locus_tag="OVW45_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCV5524844.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTESLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(61867..62565)
                     /gene="flgH"
                     /locus_tag="OVW45_000303"
     CDS             complement(61867..62565)
                     /gene="flgH"
                     /locus_tag="OVW45_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCV5524845.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(62618..63390)
                     /gene="flgG"
                     /locus_tag="OVW45_000304"
                     /pseudo
     CDS             complement(62618..63390)
                     /gene="flgG"
                     /locus_tag="OVW45_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
     gene            complement(63562..64317)
                     /gene="flgF"
                     /locus_tag="OVW45_000305"
     CDS             complement(63562..64317)
                     /gene="flgF"
                     /locus_tag="OVW45_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HCV5524846.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(64337..65542)
                     /gene="flgE"
                     /locus_tag="OVW45_000306"
     CDS             complement(64337..65542)
                     /gene="flgE"
                     /locus_tag="OVW45_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCV5524847.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(65567..66262)
                     /gene="flgD"
                     /locus_tag="OVW45_000307"
     CDS             complement(65567..66262)
                     /gene="flgD"
                     /locus_tag="OVW45_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCV5524848.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(66274..66678)
                     /gene="flgC"
                     /locus_tag="OVW45_000308"
     CDS             complement(66274..66678)
                     /gene="flgC"
                     /locus_tag="OVW45_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HCV5524849.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(66682..67098)
                     /gene="flgB"
                     /locus_tag="OVW45_000309"
     CDS             complement(66682..67098)
                     /gene="flgB"
                     /locus_tag="OVW45_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCV5524850.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            67253..67912
                     /gene="flgA"
                     /locus_tag="OVW45_000310"
     CDS             67253..67912
                     /gene="flgA"
                     /locus_tag="OVW45_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCV5524851.1"
                     /translation="MLAIKRSVAIISILFTPLSAASNLTSQLQTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRQDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVTQNARVRMVSGLVVSGVVDADGNILIN
                     Q"
     gene            67988..68281
                     /gene="flgM"
                     /locus_tag="OVW45_000311"
     CDS             67988..68281
                     /gene="flgM"
                     /locus_tag="OVW45_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCV5524852.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDASVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            68286..68702
                     /gene="flgN"
                     /locus_tag="OVW45_000312"
     CDS             68286..68702
                     /gene="flgN"
                     /locus_tag="OVW45_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCV5524853.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(68742..70277)
                     /gene="murJ"
                     /locus_tag="OVW45_000313"
     CDS             complement(68742..70277)
                     /gene="murJ"
                     /locus_tag="OVW45_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCV5524854.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(70387..71310)
                     /locus_tag="OVW45_000314"
     CDS             complement(70387..71310)
                     /locus_tag="OVW45_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCV5524855.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSADPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPILGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(71312..71959)
                     /locus_tag="OVW45_000315"
     CDS             complement(71312..71959)
                     /locus_tag="OVW45_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCV5524856.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(71970..72554)
                     /gene="rimJ"
                     /locus_tag="OVW45_000316"
     CDS             complement(71970..72554)
                     /gene="rimJ"
                     /locus_tag="OVW45_000316"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCV5524857.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            72790..73998
                     /gene="mdtH"
                     /locus_tag="OVW45_000317"
     CDS             72790..73998
                     /gene="mdtH"
                     /locus_tag="OVW45_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCV5524858.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRCRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSALLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDQGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     ATRRLLERDA"
     gene            74062..74709
                     /gene="grxB"
                     /locus_tag="OVW45_000318"
     CDS             74062..74709
                     /gene="grxB"
                     /locus_tag="OVW45_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCV5524859.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            74843..75403
                     /locus_tag="OVW45_000319"
     CDS             74843..75403
                     /locus_tag="OVW45_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCV5524860.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLISQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            75509..76555
                     /gene="pyrC"
                     /locus_tag="OVW45_000320"
     CDS             75509..76555
                     /gene="pyrC"
                     /locus_tag="OVW45_000320"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCV5524861.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKHNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            76629..76874
                     /gene="dinI"
                     /locus_tag="OVW45_000321"
     CDS             76629..76874
                     /gene="dinI"
                     /locus_tag="OVW45_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCV5524862.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            77164..77418
                     /gene="bssS"
                     /locus_tag="OVW45_000322"
     CDS             77164..77418
                     /gene="bssS"
                     /locus_tag="OVW45_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCV5524863.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            77533..78651
                     /gene="solA"
                     /locus_tag="OVW45_000323"
     CDS             77533..78651
                     /gene="solA"
                     /locus_tag="OVW45_000323"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCV5524864.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIADDFAQDKKSDFDLTPFRLSRFQ"
BASE COUNT        19349 a        20723 c        19536 g        19115 t
ORIGIN      
        1 agcgattaaa tcgcttaagg ctgccggtat cagtgtagca attgatcatt ttggcgcagg
       61 ttttgccggt ttgttactcc tgtcacgctt ccagcctgac agaattaaaa tcagtcagga
      121 attgattacc aatgttcata aaagcgggcc acggcaggca attattcaag cgatcataaa
      181 atgctgtaca tcacttgaaa ttcaagtcag tgctatgggc gtggcaacac cagaagagtg
      241 gatgtggctt gaatccgcag gaattgagat gtttcaggga gatctgtttg ctaaagctaa
      301 attgaatggt atcccttcca tagcgtggcc agagaaaaaa taattttcag cacattcttt
      361 cacatgattt cagtaaatca tcgtgaaaat gaatacgtta tgtttaaatt taatccacat
      421 taaatataat gtttcatcca atttagctga aatcttattt atactttgta caattttagc
      481 gtatattgta caaagtatta ttgggtcgtg tacaggcggc ggagatttat gaccgcaagg
      541 aggaattgtg gcttattaca gcattggtga tgttgctgaa cgttgcggga ttaatcctgt
      601 cactctccgg gcctggcaac gccgctacgg tttgttaaaa ccacagcgca gtgaaggcgg
      661 acaccgactc tttgatgaag aagacataca acgcatcgaa gagatcaagc gttggataag
      721 taatggcgtc cctgtaggca aagttaaagc attactggaa accaccagcc aggatacgga
      781 agatgactgg aaccgcctgc aagaagagat gatgtcaatt cttcgtatgg ctaatcctgc
      841 caaactacgc gcgagaatta tttcactggg tcgagagtac ccagtagatc aattgattaa
      901 tcatgtttac cttcctgttc gccagcgtct cgtgcttgat cacaacacct cccgcattat
      961 gagcagtatg tttgacggcg cattaattga atatgcagca gcctcacttt tcgaaatgcg
     1021 ccgtaagccc ggtaaagaag ccattctgat ggcgtggaat gttgaagaga gagcacgatt
     1081 gtggctggaa gcatggcgtt tatcattgtc aggatggcac atttctgttc ttgctgatcc
     1141 cattgaatcg ccgcgcccgg aactgttccc gacgcaaaca ttgattgtct ggacaggcat
     1201 ggcaccaacg agaaggcaaa acgaactttt acaacattgg ggtgagcaag gttataaagt
     1261 catctttcat gccccctaat ttcaccttta tcccgcccct tagacagaat gttataatct
     1321 gaacccacaa cagcatttgt accggagctg gtccgttgca aaatgctcac cggaagaatt
     1381 caggtaatcc ctctatcaca ctctgtacat atcacagcca aaggacagac cagattgaag
     1441 tggtaaaacc gatcgtgcac actacgcagt ctcacaacta acaagaggtt attatcctga
     1501 tggatcaggt cgttgttttt caaaagatgt ttgagcaagt gagaaaagag caaaacttct
     1561 cctggtttta ttcagaatta aaacatcacc gtattgcaca ttacatttat tatctggcta
     1621 cggataacat cagaattatt actcacgatg acacggtttt gttattaaga ggaaccagga
     1681 acctgttaaa agttagtacg accaagaacc ctgctaaaat aaaagaggcc gcattgcttc
     1741 atatttacgg aaaatctaca tttcgggaat actgttcaac actggcaggc gcgggcgttt
     1801 tccgttgggt tactgatgtt aatcataaca aacgcagtta ctacgccatt gataatacgc
     1861 ttttatatat tgaagatgta gaaaataata aaccattaat ctagctaaag ttggatgctt
     1921 aagaaatgct tcataattca gtaaggcatt agcataatgg gaataaaagt acagagacta
     1981 tccctatgga tgataaatac tgtcctttta ttgctaccca taaataatca ccaggccaat
     2041 actatcaaat tgatatttga aatgtgatca cttgactttc tatacgttat tttataacgg
     2101 ttaatatatt tataaaaaca acgggtgtgc catacgcccg tttcaatact taacgcacat
     2161 gtgttttggt ttagtcatca tccagttata tgtattttag ccaggaacag gttaaacctt
     2221 tcctatataa ctcaaaaatt gaaaccttat tctcatgtca tgtttatatt cattattatc
     2281 gttatataaa aaggcaacca taatgtttag caaattggca ccaagtagca taaaggctat
     2341 gttttaatta caggatgttc agtcatttga atgtataaca ttatagctaa acaaatctaa
     2401 aacgaagtga ataatttatt gttttcacta aatctcattt tgtttaacat ccattgagat
     2461 tccttgcttt aaattttgtt ttatataagc aatcgtttta attaatttat tttttgaagg
     2521 ggtaatatac tcatatgcaa aataaagaaa tgaacatcca aatgaactat attaaatacc
     2581 gtgggataag acataacaaa tgaagtgggt agtaattgac acggtaattc aacctacatg
     2641 cggcatatct ttttcagcca tatggggtga tatgaaaatg atcatctggt atcaatctac
     2701 tatatttctt cctcctggaa gtatatttac accggttaag cttggtatta tccttaagga
     2761 aaaagaatat cctattacta tttataacat tgcaccattc aacaagaatt tatggagttt
     2821 actcaaaagc agtcaagagt gtcctccagg agaaagcaaa ataacaaata aatgtttaca
     2881 taatagttgc attataaaaa tatgcccata tgggctcaag taatgggctt catataaata
     2941 tgctcgatat gctattaata atatccacat aaataaaaca acgggcgtgt tatacgcccg
     3001 tttcaatatt taactcatgg agagattaca tgttttcgat gatcgcttca ccaaactctg
     3061 aacatttcag cagcttagcg ccttccatca gacgttcaaa gtcataagtt acggttttcg
     3121 cgttgatagc accttccata cctttaacaa ttaaatcagc cgcttcagtc caccccatat
     3181 ggcgcagcat catctcagcg gagaggatga tagagcctgg atttactttg tcctgaccgg
     3241 catatttcgg cgcagtaccg tgggtggctt caaacagggc gcattcgtca ccgatgtttg
     3301 cgccaggggc gataccgata ccgccaacct gcgctgccag ggcgtcagaa atgtagtcac
     3361 cgttcaggtt catacaggcg ataacatcat attcagccgg acgcagcagg atctgttgca
     3421 ggaatgcatc agcaatcacg tctttaatga cgatctcttt gccggtgttc gggtttttaa
     3481 ctttcagcca cgggccgccg tcgatcagtt caccgccaaa ctcttcacgc gccagctggt
     3541 agccccagtc tttaaacgcg ccttcggtga acttcatgat gttgcctttg tgcaccagag
     3601 tcacagagtc acgatcgtta gcaattgcgt attcgatcgc tgcacgaacc agacgtttgg
     3661 tgccttcttc cgaacacggc ttaataccga taccacaatg ttccgggaag cgaattttct
     3721 tcacccccat ctcttcacgc aggaatttaa tcactttctc ggcgtcggca gagtctgctt
     3781 tccattcgat acccgcataa atgtcttccg agttttcacg gaagataacc atatcggtca
     3841 gttcagggtg tttaaccggg cttggagtgc cctgatagta acgtaccgga cgcaggcaga
     3901 tgtagagatc cagttcctgg cgcagggcaa cgttcagaga gcgaataccg ccaccaacag
     3961 gagtggtcag cggaccttta atggcaacgc gatattcacg aatcagatca agggtttcag
     4021 caggcagcca gacatcctga ccataaacct gtgtggattt ttcaccggtg taaatttcca
     4081 tccaggagat tttacgctcg cctttatagg ctttctcgac tgcagcgtcg accactttca
     4141 gcatggctgg ggttacatct acaccgattc catcaccttc aatgtaaggg ataatcggat
     4201 tttcaggaac gttgagtttg ccgttttgca gggtgatctt cttgccttgt gccggaacaa
     4261 ctactttact ttccattcac ctctccttcg agcgctactg gtttgctcgt ctgccaccac
     4321 gttgcatatg cgtttgcgtc ctgcgatacg gatgctttag agcaattttt tgttaatgat
     4381 ttgtaattgg cttgtcaatg ctaccctatt gtttgccgtc atgaaagatg cgcgttatta
     4441 ggctataatg cggcaattca taatctctga aaataccatg caaaaacttc ttttagaaat
     4501 caccaggtta agcgattcag ctcgcaacgt tctactaggc gtaaacctga aaaccagccc
     4561 acgcgtgtga tcctgttcaa taaaccctac gatgttcttc cgcagttcac cgatgaagcc
     4621 ggacgcaaaa cattaaaaga attcatcccg gttcagggcg tttatgcagc aggtcgtctt
     4681 gaccgcgata gcgaagggtt actggtgctg accaataacg gcgcgttgca ggcgcgttta
     4741 acccagccgg gcaaacgcac cgggaaaatc tattatgtgc aggtggaagg tattcccaca
     4801 caagacgcac ttgaagcctt gcgcaatggc gtaaccttaa atgatggccc taccctgccc
     4861 gccggcgcgg agctggttga cgaaccagcg tggttatggc cgcggaatcc accaattcgt
     4921 gaacgcaaaa gtattcccac cagctggctg aagatcacct tatatgaagg acgtaatcgc
     4981 caggtgcgcc gcatgaccgc ccatgttggt ttccccacgc tgcgactgat tcgttatgcg
     5041 atgggtgatt acactttgga taaccttgcc aatggcgaat ggcgagaagc aacagaataa
     5101 ggatgcacaa tgtttaaacc gcacgttacc gttgcttgcg tagtgcacgc agagggtaaa
     5161 tttttagtcg ttgaagagac gattaatggt aaagcgttat ggaaccaacc tgccgggcat
     5221 ctggaagccg atgaaacctt agtggaagct gccgcccgtg agctatggga agaaaccggc
     5281 atcagcgcgc aaccgcaaca ctttattcgt atgcatcagt ggattgcgcc agataaaacg
     5341 ccgtttttgc gtttcctctt tgccattgag cttgagcaaa tatgcccgac ccagcctcat
     5401 gacagcgata tcgactgctg tcgttgggtc agcgccgaag aaattttaaa ggcgtcaaat
     5461 cttcgttcgc cgctggtggc ggaaagtatc cgttgttatc aaagtgggca acgctatccg
     5521 ctggagatga ttggcgattt taactggcct tttacaaagg gtgtcatcta aaaggctgcg
     5581 tgatagaata cgccgccttg aagttcaatg tcgtgagtga tccaatgtct gaaaccgcaa
     5641 aaaaagtaat cgtcggcatg tccggcggtg tcgattcctc cgtttctgcc tggctgttgc
     5701 aacaacaggg atatcaggtt gaaggcctgt ttatgaagaa ctgggaagaa gacgacggtg
     5761 aggaatattg cacagcggca gcggatctgg ctgatgccca ggctgtctgc gacaagctcg
     5821 gcattgaact gcacaccgtt aactttgctg ccgagtactg ggacaatgtc ttcgaactgt
     5881 tccttgccga atataaagcc ggtcgcacgc cgaatccgga tattctgtgc aacaaagaga
     5941 tcaaatttaa agctttcctc gaatttgccg ccgaagattt aggtgccgat tatatcgcta
     6001 ccggtcatta cgtacgtcgg gccgatgtcg atggcaagag ccgcctgctg cgtggtctgg
     6061 acagcaataa agaccagagc tacttccttt atacgctcag ccatgagcag attgcgcaaa
     6121 gcctgttccc ggtcggcgaa ctggaaaaac cgcaggtgcg taagattgct gaagatcttg
     6181 gtctggtcac cgcgaagaaa aaagactcta ccggcatttg cttcattggc gaacgtaaat
     6241 tccgcgagtt cctgggccgt tatctcccgg ggcaaccggg caaaatcatt accgtcgatg
     6301 gcgatgaaat tggtgagcac caggggctga tgtatcacac tctcggtcag cgtaaaggtc
     6361 tgggtatcgg tggcaccaaa gaaggcaccg aagaaccttg gtatgtggtg gacaaagacg
     6421 tcgaaaacaa cattctggtt gtcgctcagg gccatgaaca cccgcggctg atgtctgtcg
     6481 ggttgattgc ccagcagttg cactgggtcg atcgcgaacc attcaccggc actatgcgtt
     6541 gcacggtaaa aacccgctat cgccagaccg atatcccttg caccgtcaag gcgctggacg
     6601 atgatcgcat tgaagtgatt ttcgatgaac cggttgccgc cgtgacgccg ggccagtctg
     6661 ccgtcttcta taacggtgaa gtgtgcctcg gtggcggtat tattgagcag cgtctgccgc
     6721 tgccggtctg attattatct ttacttaaaa caaggaagca gtgaacgtgg caaagaatta
     6781 ctatgacatc accctcgccc tggccggtat ttgtcagtcg gcacgcctgg tgcaacaact
     6841 cgctcaccag gggcattgtg atgccgatgc gctacacgtc tcactcaaca gtattattga
     6901 tatgaacccc agctcgacgc tggcggtttt tggcggtagc gaagccaacc tgcgcgtcgg
     6961 gctggaaacc ctgctcggcg tgctcaatgc cagcagtcgc cagggcttaa acgccgaatt
     7021 aacccgctac acactcagct tgatggtgct tgagcgcaaa ctctcctcag cgaaaggcgc
     7081 gctcgacact ctgggcaacc ggatcaacgg cctgcaacgc cagctcgaac acttcgattt
     7141 acagtccgaa acgctgatga gcgcgatggc tgctatctat gttgatgtga tcagcccgct
     7201 tggcccacgc attcaggtca ccggttcccc tgctgtactg caaagcccac aagtgcaggc
     7261 gaaagttcgc gcaaccctgc tggcaggcat tcgcgccgcc gtgctctggc accaggtcgg
     7321 cggcggacgt ctgcaactga tgttttctcg taatcgcctg accactcagg caaaacaaat
     7381 tcttgctcat ttaaccccgg agttgtgatc tatggaatta tcctcactga ccgccgtttc
     7441 ccctgtcgat ggacgctacg gcgataaagt cagcgcgctg cgcgggattt tcagcgaata
     7501 tggtttgctg aaattccgtg tacaagttga agtacgttgg ctgcaaaaac tggccgcgca
     7561 cgcagcgatc aaggaagttc ctgcttttgc tgccgacgca atcggttacc ttgatgcaat
     7621 tgtcgccaat ttcagcgaag aagatgccgc acgcatcaaa accatcgagc gtaccactaa
     7681 ccacgacgtt aaagcggttg agtatttcct gaaagaaaaa gtggcggaga tcccggaact
     7741 gcacgcggtt tctgaattca tccactttgc ctgtacttcg gaagatatca ataacctctc
     7801 ccacgcatta atgctgaaaa ccgcgcgtga tgaagtgatc ctgccgtact ggcgtcaact
     7861 gattgatggc attaaagatc tcgccgttca gtaccgcgat atcccgctgc tgtctcgtac
     7921 ccacggtcag ccagccacgc cgtcaaccat cggtaaagag atggcaaacg tcgcctaccg
     7981 tatggagcgc cagtaccgcc agcttaacca ggtggagatc ctcggcaaaa tcaacggcgc
     8041 ggtcggtaac tataacgccc acatcgccgc ttacccggaa gttgactggc atcagttcag
     8101 cgaagagttc gtcacctcgc tgggtattca atggaacccg tacactaccc agattgaacc
     8161 gcacgactac atcgccgagc tgtttgattg cgttgcacgc ttcaacacca ttctgatcga
     8221 ctttgatcgt gacgtctggg gttatatcgc ccttaaccac ttcaaacaga aaaccattgc
     8281 tggtgagatt ggttcttcca ccatgccgca taaagttaac ccgatcgact tcgagaactc
     8341 cgaagggaat ctgggccttt ccaacgcggt attacagcat ctggcaagca aactgccggt
     8401 ttcccgctgg cagcgtgacc tgaccgactc caccgtgctg cgtaacctcg gcgtgggtat
     8461 cggttatgcg ctgattgcgt atcaatccac cctgaaaggc gtgagcaaac tggaagtgaa
     8521 ccgtgaccat ctgctggatg agctggatca caactgggaa gtgctggctg agccaatcca
     8581 gacagttatg cgtcgctatg gcatcgaaaa accgtacgag aagctgaaag agctgactcg
     8641 tggtaagcgc gttgacgccg aaggcatgaa gcagtttatc gacggtctgg cgttgccaga
     8701 agaagagaaa gcccgcctga aagcgatgac gccggcaaac tacattggtc gcgccatcac
     8761 catggttgat gagctgaaat aaactcgtat cagtgccgga tggcgatgct gtccggcctg
     8821 cttattaaga ttatccgctt tttatttttt cactttacct cccctccccg ctggtttatt
     8881 taatgtttac ccccataacc acataatcgc gttacactat tttaataatt aagacaggga
     8941 gaaataaaaa tgcgcgtact ggttgttgaa gacaatgcgt tgttacgtca ccaccttaaa
     9001 gttcagattc aggatgctgg tcatcaggtc gatgatgcag aagatgccaa agaagccgat
     9061 tattatctca atgaacattt accggatatt gcgattgtcg atctcggatt gccagacgag
     9121 gacggtctgt cactgattcg ccgctggcgt agcaatgatg tttcactgcc gattctggta
     9181 ttaaccgccc gtgaaagctg gcaggacaaa gtcgaagtat taagtgctgg tgctgatgat
     9241 tacgtgacta aaccatttca tattgaagag gtgatggcgc gaatgcaggc attaatgcgg
     9301 cgtaatagcg gtctggcttc acaggtcatt tcgctaccac cgtttcaggt tgatctctct
     9361 cgccgtgaat tatctattaa tgacgaagtg atcaaactga ccgcgttcga atacaccatt
     9421 atggaaacgt tgatacgcaa taatggcaaa gtggtgagca aagattcgtt aatgctccaa
     9481 ctctatccgg atgcggagct gcgggaaagc cataccattg atgtactgat gggacgtctg
     9541 cgcaaaaaaa ttcaggcaca atatccgcaa gaagtgatta ctaccgttcg cggccagggc
     9601 tatttgttcg aattgcgctg atgaaaaaat tactgcgtct ttttttcccg ctctcgctgc
     9661 gggtacgttt tctgttggca acggctgcgg tagtactggt gctttcgctt gcctacggaa
     9721 tggtcgcgct gatcggttat agcgtcagtt tcgataaaac tacgtttcgg ctgttacgtg
     9781 gcgagagcaa tctgttctat acccttgcga agtgggaaaa caataagttg catgtcgagt
     9841 tacccgaaaa tatcgacaag caaagcccca ccatgacgct aatttatgat gagaacgggc
     9901 agcttttatg ggcgcaacgt gacgtgccct ggctgatgaa gatgatccag cctgactggc
     9961 tgaaatcgaa tggttttcat gaaattgaag cggatgttaa cgataccagc ctcttgctga
    10021 gtggagatca ttcgatacag caacagttgc aggaagtgcg ggaagatgat gacgacgcgg
    10081 agatgaccca ctcggtggca gtaaacgtct acccggcaac atcgcggatg ccaaaattaa
    10141 ccattgtggt ggtggatacc attccggtgg agctaaaaag ttcctatatg gtctggagct
    10201 ggtttatcta tgtgctctca gccaatctgc tgttagtgat cccgctgctg tgggtcgccg
    10261 cctggtggag tttacgcccc atcgaagccc tggcaaaaga agtccgcgaa ctggaagaac
    10321 ataaccgcga attgctcaat ccagccacaa cgcgagaact gaccagtctg gtacgaaacc
    10381 tgaaccgatt gttaaaaagt gaacgcgaac gttacgacaa ataccgtacg acgctcaccg
    10441 acctgaccca tagtctgaaa acgccactgg cggtgctgca aagtacgctg cgttctctgc
    10501 gtagtgaaaa gatgagcgtc agtgatgctg agccggtaat gctggagcaa atcagccgca
    10561 tttcacagca aattggctac tacctgcatc gtgccagtat gcgcggcggg acattgctca
    10621 gccgcgagct gcatccggtc gccccactgc tggacaatct cacctcagcg ctgaacaaag
    10681 tgtatcaacg caaaggggtc aatatctctc tcgatatttc gccagagatc agctttgtcg
    10741 gtgagcagaa cgattttgtc gaggtgatgg gcaacgtgct ggataatgcc tgtaaatatt
    10801 gcctcgagtt tgtcgaaatt tctgcaaggc aaaccgacga gcatctctat attgtggtcg
    10861 aggatgatgg ccccggtatt ccattaagca agcgagaggt cattttcgac cgtggtcaac
    10921 gggttgatac tttacgccct gggcaaggtg tagggctggc ggtagcccgc gaaatcaccg
    10981 agcaatatga gggtaaaatc gtcgccggag agagcatgct gggcggtgcg cggatggagg
    11041 tgatttttgg tcgccagcat tctgcgccga aagatgaata aatatgtcca tacttcacgc
    11101 attacgttaa gcatccgtta taatcggttg cagataccag cctgtggatg cttaacatgg
    11161 aataccaact cactcttaac tggcccgatt ttcttgaacg tcactggcag aaacgcccgg
    11221 tggtgttaaa acgcggcttt aataatttta ttgacccgat ctctccagac gagttggcgg
    11281 gtctggcgat ggaaagcgaa gttgacagtc gactggtcag tcaccaggat ggcaaatggc
    11341 aggtcagcca cggcccgttc gaaagctacg atcatctcgg tgaaaccaac tggtcattac
    11401 tggtacaggc agtgaaccac tggcatgagc cgaccgccgc gctgatgcga ccgttccgtg
    11461 aactaccgga ctggcgtatt gatgatctga tgatttcttt ttctgtacct ggcggcggcg
    11521 tcggcccgca tctcgatcag tacgacgtgt ttatcattca gggtaccgga cgtcgtcgct
    11581 ggcgagtggg cgaaaagctg caaatgaaac agcactgccc acatccggat ctgttacagg
    11641 tcgatccgtt cgaagccatc atcgatgaag agctggagcc tggtgatatt ctttatattc
    11701 cgccaggatt cccgcatgaa ggctacgcgc tggaaaatgc gatgaactat tccgtgggct
    11761 ttcgcgcgcc aaatacgcgg gaactgatta gtggatttgc cgattatgtg ctgcaacgtg
    11821 aactgggcgg caactactac agcgatcctg atgttccacc tcgcgctcat cctgcggatg
    11881 ttctgcctca agagatggat aaactgcgtg agatgatgct cgaattgatc aaccagccgg
    11941 aacactttaa gcaatggttt ggcgagttta tatcccagtc acgtcatgaa ctggatatcg
    12001 cgccgccaga accgccttat cagccagatg aaatctacga tgcgctgaaa caaggtgatg
    12061 tgctggtgcg cctgggtggt ctgcgcgtat tgcgcattgg cgacgacgtg tatgccaatg
    12121 gtgagaagat cgattccccg caccgtccgg cactggatgc actcgccagc aacattgcgc
    12181 tgactgcgga gaattttggc gatgcgctgg aagatccgtc attcctcgcg atgctcgcgg
    12241 cgctggtcaa tagcgggtat tggttcttcg aagggtaagt gtggatttaa gccggatgcg
    12301 gcatcacatg ccgcatccct tttcgcttac ttccgttgcg ccgttaactc ggcaatacgg
    12361 acgatcacct gcaccgcttt ttccatacct tccagagtca caaactcatg cttaccatga
    12421 tagttgtaac cgccagtgaa caagttcggg cacggtaatc ccataaacga caactgcgcg
    12481 ccgtcggtac caccgcggat cggtttcagt tccggttcaa tatcgcagtc acgcatcgcc
    12541 tgctgggcga tatcgagaat atgcggatgc tcaaccactt tctctcgcat attgtagtaa
    12601 ctgtcttcaa tcaccagttc aatgtagcaa tcaggatgta accctttgcc cacttttttg
    12661 gcgatctcca tcattttacg tttacgcgct tcaaactgtt tacggtcgaa atcacggatg
    12721 atgtagtgca tatcggcccg ttcaacggtg cctttcatgc tcgccaggtg atagaaacct
    12781 tcatagcctt ctgtcatttc cgggctttca tccgccggaa cttccgcatg aatacgtgcc
    12841 gccagcgaca gcgcatttac catcactcct ttcgccgtgc ccggatgaac attgttaccg
    12901 acaattttga tattgactga tgcggcgttg aagttttcaa actccagttc gcctacgcca
    12961 ccaccgtcaa cagtgtaagc ccagcgggca tcgaaggcat caacatcaaa atgtttcgcc
    13021 cctttgccca cttcttcatc cggggtaaag gcgacgcgaa tatcaccatg cggaatgttt
    13081 ttctgttgca ataccgccag cgcggtcatg atttctgcaa tacctgcttt gtcatcggca
    13141 cctaacaagg ttttaccatc ggtggtaatc aacgtctgac ccagtagctg atgcagcacc
    13201 gggaacataa ccggtgataa aacttcatcg ccgataccca gcgcaatatc gccaccgcga
    13261 tagttttcaa caatttgcgg attaacattt ttgccgctgc aatccggtga ggtatccaca
    13321 tgagaaataa agccaatcgc cgggatatcg ccagggacgt tagccggtaa cgtcgccatc
    13381 aaagtgccct tctcacttaa ggtcacattg ataagcccca tttcttcgag ctgctctttc
    13441 agcagatgca ataacttcca ttggccttcc gtgctgggaa cctgtctcac ccctgctttt
    13501 gattgggtat ccagagacac gtagttcaaa aatcgctcaa gtagtttatc catgtagtca
    13561 ccctcacttt ttgtgacaac attattaaga agcaagaaaa gacaaatatt gcgtcaggtc
    13621 acttttaccc ctgcaagcgg gaatatttat cagcattacc ttaatgaata taaagctaag
    13681 ccagtaattc acaacaaaga ttggcgattc aagcggtttg ccgtagaatt accgccctca
    13741 ttaagagtca ccaaggtggt taaccacaaa ccccgcatcg gtaagccatc cgttgcgttt
    13801 acatgggaca gagtaaaaaa ttgaataaac aaccgagttc gctttcaccg ctggtgcaat
    13861 tggcgggaat tcgcaaatgc tttgatggta aagaggtcat tccccagctg gatctgacta
    13921 tcaacaatgg cgagttcctc acgctgcttg gcccttctgg ctgcggtaaa acaaccgttc
    13981 ttcgcctgat tgcaggtctg gaaactgttg attccggacg catcatgctg gataacgagg
    14041 acatcaccca cgttccggcg gaaaaccgct atgtgaacac tgttttccaa agctacgcac
    14101 ttttccccca catgaccgtg ttcgaaaatg tggcctttgg gttgcgcatg caaaaaaccc
    14161 ccgctgctga aattacgccc cgcgtgatgg aagccctgcg gatggtgcaa ctggaaacct
    14221 tcgctcaacg taaaccgcat cagctctctg gtggtcaaca gcaacgcgtc gccattgctc
    14281 gcgcggtggt taacaagcct cgtctgttgt tgctggatga gtcgctctca gcgctggatt
    14341 acaaactgcg taagcaaatg cagaacgagc tgaaagcgtt acagcgtaag cttggtatta
    14401 ccttcgtctt tgtcactcac gatcaggaag aagcgctcac catgtcagac aggattgtgg
    14461 tgatgcgcga gggtcgtatt gagcaagatg gtacaccgcg cgaaatttac gaagagccga
    14521 aaaacctgtt tgttgccggc ttcattggcg aaatcaatat gtttaacgcc actgtcatcg
    14581 aacgtctcga cgagcagcgc gtacgcgcca acgttgaagg ccgcgaatgt aatatctacg
    14641 ttaacttcgc cgttgaaccg gggcaaaaac tgcatgttct gctgcgcccg gaagacttac
    14701 gtgttgaaga gattaacgac gacaaccacg ctgaagggct gattggttac gttcgtgagc
    14761 gtaactacaa aggcatgacg ctggagtcgg ttgttgaact ggaaaatggc aagatggtga
    14821 tggtcagcga attcttcaat gaagacgacc ctgactttga ccactctctc gaccaaaaaa
    14881 tggccattaa ttgggtagaa agctgggagg tcgtactggc tgatgaagaa cacaagtaag
    14941 ttccagaatg tagtgattgt cactattgtc ggttggcttg tgttgtttgt ctttctgccc
    15001 aacctgatga tcattggcac cagctttttg acccgcgacg acgccagttt cgtcaaaatg
    15061 gtctttacgc tggataacta cacgcgtctg ctcgatccgc tctattttga agtgctattg
    15121 cactcgctga atatggcgct gatcgccacc ctcgcctgcc tggtgctggg ctacccgttt
    15181 gcctggtttc tggcgaagct gccacacaag gtgcgtccgc tgctgctgtt tctgctgatt
    15241 gttcccttct ggaccaactc actgattcgc atctacgggc tgaaaatttt cctcagcacc
    15301 aaaggctatc tcaacgagtt tttgctctgg ctgggcgtta tcgacacacc aattcgtatc
    15361 atgttcacgc ccagtgcggt gattatcggt ctggtttaca ttctgctacc gtttatggtg
    15421 atgccgctgt actccagtat cgaaaaactg gataagccgt tgcttgaggc ggcgcgcgat
    15481 ctcggtgcca gcaagttaca gacttttatc cgtatcatta ttccactgac gatgccggga
    15541 attattgccg gatgtctgct ggtgatgctg ccagcgatgg gcctgttcta tgtatccgac
    15601 ctgatgggcg gtgcgaaaaa cctgctgatc ggtaacgtca tcaaggtcca gttcctcaat
    15661 attcgtgact ggccgtttgg tgcagccacc agcattacgc tgactatcgt aatgggcctg
    15721 atgttgctgg tttactggcg cgcttctcgt ctgctgaata aaaaggtgga actcgaatga
    15781 tcggtcgact gcttcgcggc ggttttatga ccgctatcta cgcgtacctg tatatcccaa
    15841 tcattatttt gattgtgaac tcctttaaca gctcgcgctt tggcatcaac tggcagggtt
    15901 ttaccaccaa atggtatagc ctgctgatga acaacgacag cctgttacag gcagcgcagc
    15961 attcactaac aatggcggtg ttttcggcga catttgctac gcttatcggt tcactgacgg
    16021 cagttgcgct gtaccgttat cgctttcgtg gtaagccgtt cgttagcgga atgctgtttg
    16081 tagtgatgat gtcaccagat atcgtgatgg cgatttctct gctggtgctg tttatgctgc
    16141 tgggtattca gcttggcttc tggtcgctgc tgttctcgca tatcaccttc tgcctgccgt
    16201 ttgtggtggt gacggtgtat tcgcgcctga aaggttttga cgtgcggatg ctggaagcgg
    16261 cgaaagatct cggtgccagt gaatttacca ttctgcgaaa aatcattctg ccactggcaa
    16321 tgccagcggt ggcggcgggc tgggtgttaa gctttaccct gtcgatggac gatgtggtag
    16381 tttcttcgtt cgtcaccgga ccaagttatg aaattctgcc gttaaaaatt tattcgatgg
    16441 tcaaagtcgg cgtatcgccg gaagttaacg cgctggcaac catattactg gtgctgtcgc
    16501 tggtgatggt tattgccagc cagcttattg ctcgtgataa aacgaaaggt aacacagggg
    16561 acgttaaatg aaaaaatggt cacgccacct gctcgcggcg ggtgctctgg cactgggcat
    16621 gagcgccgct cacgccgatg acaacaacac gctgtatttc tacaactgga ccgagtacgt
    16681 gccgccagga ctgcttgaac agttcaccaa agaaaccggt attaaggtta tctattcgac
    16741 ttacgagtcg aacgaaacca tgtacgccaa gctgaaaacg tacaaagacg gtgcctatga
    16801 cctggtggtt ccttcaacct attacgtcga taaaatgcgt aaagaaggga tgatccagaa
    16861 gatcgacaag tcgaagttaa cgaacttcag caatctcgat ccagacatgc tcaacaagcc
    16921 gtttgacccg aataacgact actccattcc gtatatctgg ggtgcgacgg cgattggcgt
    16981 taacggtgat gcggtggatc ctaaatctgt caccagttgg gcagatctgt ggaagccaga
    17041 gtacaaaggt agcctgctgc tgaccgacga tgcccgtgaa gtgttccaga tggcgctgcg
    17101 caaactgggc tactccggta acaccaccga tccgaaagag attgaagctg catataacga
    17161 gctgaaaaaa ctgatgccaa acgtcgcagc gtttaactcc gataacccgg ctaacccgta
    17221 catggaaggc gaagttaacc tcggcatgat ctggaacggc tctgctttcg ttgcacgcca
    17281 ggcgggtacg ccaattgacg tggtgtggcc gaaagaaggc ggcattttct ggatggacag
    17341 cctggcgatc ccggcaaatg ccaaaaacaa agaaggcgcg ctgaaattga tcaacttcct
    17401 gctgcgcccg gatgtggcaa aacaggttgc cgaaactatc ggttatccaa cgccaaacct
    17461 tgcggcgcgt aagctgttaa gcccggaagt ggcgaacgat aaaacgctct acccggatgc
    17521 tgaaaccatt aaaaatggtg aatggcagaa tgacgttggc gcagccagca gcatttatga
    17581 agagtattat cagaagctga aagcaggacg ttaattcagc aaacgggcgg tggtattacc
    17641 gcccgttttt tagttagcaa ggatgtggct aatgagtcag gacagtaaag ttttatcccg
    17701 ctctttcgtc ggtattgtgg tccccttgta ggcctgataa gcgtagcgca tcaggcaatg
    17761 cttcctgctt tataatccct tcagcaactt ttcaacaaac tcaggcacca cctggcttgc
    17821 cgggccgtaa tatttctcgg caaattcatt accgacctga cttggttcga gattcagttc
    17881 cacggtgtgc gcgccatgca gtttcgcttc gtgaacaaac ccagccgccg gataaacatg
    17941 cccggaagta ccaatggcaa tgaaaatatc agccatcgac aacgccatat aaatttcatc
    18001 catgccgagt ggcatttcgc caaaccaaac gacgtgcggg cgcaagggtg ccgggaactg
    18061 gcaacaatgg catttatctt ctggcgtaac gtctccggtc cagtcgagaa cctgaccact
    18121 ttgcgaacaa cgaactttca gcaattcccc atgcatatga atgacattgg tattacctgc
    18181 gcgttcatgc aggttgtcta tattctgcgt taccagcaaa aagcgatcgc cgagggcgtc
    18241 ttgcagttta gccagcgcaa gatgcgcggc gttaggctga atttctggct gctgcagctg
    18301 tcgacgacgg gcgttataaa acgcttgcac cagttcagga tcgcgatcga aaccttccgg
    18361 cgttgccaca tcttcaaccc gatgttcttc ccacaggcca tctgcggcgc gaaaggtacg
    18421 aatacctgat tccgcagaaa ttcctgcccc tgtcagtacg agtactcttg gtttttccat
    18481 tgcttccggc accactttat ctctgaaaaa aatacgctga cgcaaacgct cgcgcaggcg
    18541 gcgtttattt ttacgaaaac gacttaaccg atgaccccga cgcgacagca tagcaacctc
    18601 tgttgtttaa tcggttagat gtaggaaggc cgcaccacgc attcctcccg catcaccgtg
    18661 gcgcgcgcgt tcaatgcgcg gaacacgagc tacaggtaag agatgacgag gcagcctgtc
    18721 cgccagttgc gttgtgattg ccgggaaatt cgataagcca ccaccaatga cgaccaggtc
    18781 agagtcaacg atggtcagga tatttcccag acaaaccgct aataaatcca gataacgctc
    18841 aacgtgcccc ttgcctgctc atcgccttga tcataaagcg caataatttc gggagcctgc
    18901 aacggttgat gataatagtg ttgccagagc cacgcaaagc cccgacctga tagataattt
    18961 tcgatacaac caatttgacc acagccgcag cggcgtaacg ggaaatccag ccccatcatg
    19021 gttaacgcat caaccggcag acgcatatgg ccaaactcgc cggtaatgta gcttttaccg
    19081 gtaatcggtt taccgttgaa aatcagcccg ccgccaacgc cggtgccgag aattaacccc
    19141 atcaccagtg gatattgcgt aaattcatca tcccaggctt ctgaaagggc aaaacagttg
    19201 gcatcgttat caaggcgtac atcgcgatca agacgtgcgc tcaggtcggc acgcagcggt
    19261 ttaccgctgg cagcggggac attggcggca tacagcgtac catcttctgt ttccggcatt
    19321 cccggaatac cgatgccaac agaaccttta cagccaaaac gttggtcagc ttcggctacc
    19381 agctcgcata ctgcatctaa aaatgcgtca tagctgtcac gcggtgtcgg cacccgcttt
    19441 tcccactgca actgccgacc gctatcaaac acgccaagcg caatttttgt tccaccaata
    19501 tcaaacccgt aatacattgc cgctccttgt tttaatgtac tgcctttact ggccgctaag
    19561 gactcgcgca gggtcaatat tactggcgcg ccgcgccgga taccaacttg ccaaaagact
    19621 cagcaacaat gctgtgacca gtacgtagaa gacgtccagc cagtgcaatt ccgatggcag
    19681 gaagtcaata aaatagatat cgctggagag gaactgatga ccgatcagct tttcaatccg
    19741 ctcaataatc ggggtaagtt gcagtgaaac aactacgccg ataatcacac cacacaggct
    19801 accgaacagc cctgccagca atccatacca gacaaagatg gcgcgaatta aaccatcttt
    19861 cgcccccagc gttcttaata ctgcgatatc gccactcttg tctttcaccg ccatcactaa
    19921 ggtggagacg atgttgaaac aggccacgcc aatcaccagt accatcgcca gatacataat
    19981 ggcgcggatc atctggatat cgcgatacat atagccgtaa gtaccaatcc aacttttaat
    20041 ataaacatag ctgttggtca cttcacccgc atcgcgtacc agcttattgg cgttgaaaac
    20101 atccgtcatt ttaagggcaa tacctgacac gctggaaccc atatcaagat attgttgggc
    20161 atccgccagc gggatcatgg caaaactgtg atcgagttga ccactcaact gcaaaatacc
    20221 ggcaatgtgc aaacgcacac gttttggctg catcagttta tgctcgggat tcgagttggg
    20281 gatcatgatc gacacccaat cgccctgctt cactttcagc gcatccgcca cgcctttgcc
    20341 gatgataatt tgctgttcgc ccgctttaaa attgcgccag gcatccccct gaacaaacga
    20401 gggtaatgcg ctcagacgct gttcctgttg cgggttaacg cccttcacct gaattgcgcg
    20461 cagattggta ccactttcta ccagtccggt gaaattgata tacggcgcag cggcggcaat
    20521 acccggcact ttttgcacgt tatccagtgc ttcctgccag ttagtccatg gctgatttac
    20581 cgcctcaatt tcaccgtgcg gtaccacagc aagaatgcgg ttattcagtt cgcgttcaaa
    20641 gccgttcatc gcgcttaagc cgacgatcaa taccgccacg ccgagggcaa tgccaatggt
    20701 agaaatgaca gagattagcg acaccatgcc gccgcgtcgc cgtccgcgac taaaacgcag
    20761 gccaatcaat aacgataaag gcatcgccat tactccgccc ccatcaggct cagttccgcc
    20821 gtcagacgtc catcacgcat ttccagttgg cggctcatac gtttcgccag ttgcaggtcg
    20881 tgagtaacca ccaggaaggc ggtgccctgc aagcgattca attccccaag caactggaag
    20941 atgctgtcgg cgttacgcgc atcgaggtta ccggtaggct catccgcaag taccagacgc
    21001 ggattattga ccagcgcacg ggcaatcgcc acacgctggc gttcgccgcc agaaagttca
    21061 gatgggcggt gattcgcacg atgctccagc cccaccgctt ttaacatctc cagcgcacgg
    21121 ctgttgattt cagcgggctt tttcttgcca atcagcagtg gcatagccac gttttccagg
    21181 gcagtaaaat ccggcagcag gtggtgaaac tgataaataa agcccagctt ctggttgcgc
    21241 agttctgctt tcgccgccga agacagtttg ctcatcggtt gaccgttaaa gatcacatcg
    21301 ccggaggttg gtgtatccag cccgcccagc aggtgcagca aggtactttt accggaacca
    21361 gagctaccga cgatcgccat catttcacct tcgccgacgc tgaaactgac attgtgcagt
    21421 acatcggttt gcacactgcc ttcctgatag cgtttgcaca ggttgtcgca ttgcaacagg
    21481 atcttattca taacgtaaag cctcagcggg ttgagtggcg gcagcgcgcc atgaagggta
    21541 aagcgtagac agcagcgcga tagccatcgc caccagcgca ataacaatga cctgtaaagg
    21601 ttcgatagcg accggcagcg ccgcgccatc aagcaggacg ccgattatcg gcatcagatt
    21661 atttaactgg ctggcaagca gtgcgccaag cgccgctccg aggatcgcac cgataatccc
    21721 ggcgctggcc ccttgcacca taaagaccat catgatttgt cgcggagtta agccttgcgt
    21781 ttgcaggatc gcgacttcgc cctgcttctc cattaccatc agccctagtg aggtaataat
    21841 gttaaacgcc gcaacggcga caatcaggct cagcagcaag cccatcatat ttttttccat
    21901 gcgtacggcc tggaacagct cgcctttacg gtcacgccag tcctgccatt tgctgccttc
    21961 aggcagtttt tgctgactta aagagtcaac tttcagcggc tcatccagcc acaaacgcca
    22021 gccggtaata ttgcctgccg gataacgcat cagacgcgag gcatcctcaa tattcaccag
    22081 catttcatag ccatcgactt cactgttggc ggcgaaagtg ccaatcacat tgaacaggcg
    22141 ctggcttgga atacgcccca tcggcgtgaa ctggctggca gatggtacca tcacgcggat
    22201 ttgatcaccg cgattaacgc ctagctgtga ggcaagttgt tcgccgagga tgacattata
    22261 tttccccggc tcgaggtcag tttgtttcac attgaccaga tacggcgtaa gtggatcttt
    22321 ttgcgccggg tcgataccga gcatcacccc gaccgccacg ctgcgcgcgc tttgcagtac
    22381 cacatcaccg gtagtaatag gtgcgacgcg attaacgccg tccagtttga ctgccgtttc
    22441 cgggagttgc tgcgggttaa gagagccatg ctcagaagag agaattgcct gtggcatcag
    22501 gccaaggatg ttgttttgca gctcgcgctc aaagccgttc atcactgaca atactgtgac
    22561 cagcgccatc accccgaggg taatgccgat ggtagaaagc caggagacga aacgaccgaa
    22621 gcgatccgct gcacgcccac gcatgtaacg caggccaata aatagagcga caggttggta
    22681 catgaaatcc gtctggttgc tgtagcaaag acacgagtat ataagcgaaa gcaagatggg
    22741 taaacgtctg aactgtaagt gatcgtcagg gctaatctga aaagcccccg taaatcaatc
    22801 ggctaaattg ccatgtttta tcatcaatct tgccgcctga tcttaatctg aaaaatcatc
    22861 caatacagac agatacccat tacacctcgg gtcataaccc gcaggatcaa ccgcatgtaa
    22921 attaaaaaag agcgtgttgt cctgatgaaa caaaaagagc tatggattaa ccagatcaaa
    22981 gggttatgta tttgtctggt ggtgatttat cactcggtca ttacctttta tccgcatatg
    23041 accacttttc agcatccgtt atcggaagtc ctgagcaaat gctggatcta tttcaatctt
    23101 taccttgcac cctttcgtat gccggttttt ttctttatat ccggctattt gattcgccgc
    23161 tatatcgaca gcgtaccgtg gggaaattgt ctcgataaac ggatctggaa catcttctgg
    23221 gtgctggcgc tttggggcgt ggtgcagtgg ctggcgttaa gtgcgctgaa ccagtggctg
    23281 gcacctgagc gcgatttaag caatgcctcc aatgccgctt atgccgattc caccggtgag
    23341 ttcctgcacg ggatgatcac cgccagcacc agcttgtggt atctgtatgc gttaattgtc
    23401 tatttcgtgg tatgtaaaat ttttagccgt ctggcgctgc cactattcgc cttgtttgta
    23461 ctgctgagtg tggcggttaa tttcgttccc acgccgtggt ggggaatgaa cagtgtgatc
    23521 cgcaatttgc cttattacag ccttggcgca tggtttggcg caacaataat gacctgtgtt
    23581 aaagaggtgc cgttgcgccg ccatctgctg atggcttctt tgcttaccgt tctggcggtc
    23641 ggtgcctggt tgtttactat ctcgctgctg ttgtcgctgg tatcgattgt ggtgatcatg
    23701 aagctgtttt atcagtacga gcaacgattc ggtatgcgct ccaccagcct gctgaatgtg
    23761 attggctcca acaccattgc tatctacacc acccatcgca ttctggttga aatattcagc
    23821 ttaactctgc ttgcgcaaat gaacgcagca cgctggtcgc cgcaagtcga actgacactc
    23881 ctgctggttt atccctttgt cagtttgttc atctgtactg ttgcgggctt gctggtaaga
    23941 aaactttcac agcgtgcatt cagcgatctg ttgttctccc cgccttcact gcccgctgcc
    24001 gtcagttact ctcgttaaac catcgcccca gccggggcga ttcaatttgc taaaccatgt
    24061 cgattacgga taatgaacag cattgcttat cagagatatg cccccatatg ttgaggcata
    24121 tcctaacgag aatctgacaa ccgttatgcc tgaacaatat cgttatacac tgcccgtcaa
    24181 agcgggtgag cagcgtctgc tgggcgagtt aaccggcgca gcctgtgcaa cgctggtagc
    24241 ggaaattgcc gaacgtcacg ccggtccggt ggtactcatt gcaccagata tgcaaaatgc
    24301 tctgcgtttg catgatgaaa tcagccagtt caccgatcag atggtgatga atctggcgga
    24361 ctgggaaact cttccctacg acagtttttc gcctcatcag gacattatct cctcgcgcct
    24421 ttccaccctt taccagctac cgacgatgca gcgtggcgta ctgattgttc cggtgaatac
    24481 gcttatgcag cgcgtttgcc cacacagttt tctccacggt catgcgctgg tgatgaaaaa
    24541 aggtcagcgc ctgtcacgag atgcattacg aacccaactg gacagcgccg gttatcgcca
    24601 tgttgaccag gtgatggagc acggcgaata cgccacgcgc ggcgcgttgc tggatctctt
    24661 cccgatgggg agtgagctgc cttatcgtct tgatttcttt gatgatgaaa tcgacagcct
    24721 gcgggtgttt gacgtcgaca gccagcgcac gctggaggaa gtagaagcga tcaatctgct
    24781 gcccgcgcac gaatttccga ccgataaagc ggcaattgaa ctgttccgca gccagtggcg
    24841 cgataccttc gaagtgaagc gcgatccgga acatatttac cagcaagtga gtaaaggcac
    24901 attacctgcc gggatcgagt actggcagcc attgttcttc agcgaaccac tgccgccgct
    24961 gttcagttat ttccctgcca ataccttgct cgtgaatact ggcgatctgg aaaccagtgc
    25021 cgaacgtttc caggctgaca cgctggcgcg ttttgagaat cgcggcgtcg atccgatgcg
    25081 cccgctgttg ccaccacaat cgctctggct gcgggtggac gagctcttct cagagctgaa
    25141 aaactggccc cgggtgcagc taaaaactga acatttaccg acaaaagccg cgaatgccaa
    25201 tttaggtttc cagaaactgc cagacctggc cgttcaggca caacaaaaag cgccgctgga
    25261 tgcgctgcgt aagttcctcg agactttcga cggtccggtg gtgttctcgg tagaaagtga
    25321 aggtcgccgt gaagcgctgg gtgaactgct cgcacgaatt aaaattgctc cgcaacgcat
    25381 tatgcgtctt gatgaagcca gcgaccgtgg gcgttatctg atgattggcg ctgccgaaca
    25441 tggttttgtc gataaggtgc gtaatctggc gctgatctgc gaaagcgatc tgctcggtga
    25501 acgtgttgcc cgccgtcgtc aggattctcg ccgcaccatc aaccccgata cactgatccg
    25561 taaccttgcg gagctgcata ttggtcagcc ggtggtccat ctggagcacg gtgtcgggcg
    25621 ctatgccgga atgaccacgc tcgaagcggg cggcattact ggcgagtatt tgatgctcac
    25681 ctatgccaac gacgccaaac tgtatgttcc ggtgtcgtca ctgcatctga ttagccgtta
    25741 cgcaggtggc gcggaagaaa acgccccgct gcataaactt ggcggcgatg cgtggtcacg
    25801 cgcgcggcag aaagcggcgg aaaaagtgcg tgatgtggcg gcggaattgc tggatatcta
    25861 cgcgcaacgc gccgccaaag agggcttcgc gtttaaacac gatcgtgagc agtatcagtt
    25921 gttctgcgac agcttcccgt ttgaaaccac gccggatcag gcgcaggcca ttaatgcggt
    25981 acttagcgac atgtgtcagc cgctggcaat ggatcgtctg gtgtgcggcg atgttggctt
    26041 tggtaaaaca gaagtggcga tgcgcgccgc tttcctggca gtagataacc acaagcaggt
    26101 ggcggtgttg gtgcctacca cccttctcgc gcagcagcat tacgacaact tccgcgaccg
    26161 tttcgccaac tggccggtac gtatcgaaat gatctcccgt ttccgcagcg ccaaagagca
    26221 gacgcaaatc cttgcggaag tggcggaagg gaaaatcgat attctgatcg gtacgcacaa
    26281 actgctgcaa agtgacgtca agtttaaaga tttaggcctg ctgattgtcg atgaagaaca
    26341 ccgcttcggg gtgcgtcata aagagcgcat taaagcgatg cgcgcgaacg tggatattct
    26401 gacgcttact gcaacgccga tcccacgcac gctgaatatg gcaatgagcg gaatgcgtga
    26461 cctgtcgatt atcgccacgc cgcccgcccg tcgtctggca gttaaaacct ttgtccgtga
    26521 gtatgacagc ctggtggtcc gggaggcgat cctgcgtgaa attttgcgcg gggggcaggt
    26581 ttattatctc tacaatgatg tggaaaacat ccagaaagct gccgaacggc tggcagaact
    26641 ggtgccagaa gcgcggatcg ccatcggtca cgggcagatg cgcgagcgcg aactggaacg
    26701 ggtgatgaat gatttccatc atcaacgttt caacgtgctg gtttgtacaa ccattatcga
    26761 aaccgggatc gacatcccga cagccaacac cattatcatt gaacgcgcgg atcacttcgg
    26821 tctggcgcag ctgcaccagt tacgcggtcg cgtcggacgt tcgcatcatc aggcatatgc
    26881 atggttgctg acaccgcatc caaaagcgat gactaccgat gcacaaaaac gtcttgaagc
    26941 gattgcctcg ctggaagatc tcggggcagg ttttgcgctg gcaacgcacg atctggagat
    27001 tcgcggcgcg ggtgaactgc ttggcgaaga acaaagcggc tcaatggaaa ccatcggttt
    27061 ctcgctgtat atggagttgc tggaaaacgc cgtcgatgca ctgaaagccg gacgcgagcc
    27121 gtcgctggaa gatctcacca gccagcaaac agaagtcgag ctgcggatgc cgtcgctatt
    27181 gccagatgat ttcattcctg acgtgaatac gcgtttgtcg ttctataaac gtattgccag
    27241 cgccaaaacg gaaaacgaac tggaagagat caaagtcgag cttatcgatc gcttcggcct
    27301 gctgccagat ccggcgcgta ccctgctgga tattgcccgt ctgcgccagc aagcgcagaa
    27361 actggggatc aggaagctgg aaggtaatga gaaaggcggg gtgatcgaat ttgccgagaa
    27421 gaatcacgtt aatccggcct ggttgattgg tttgctgcaa aaacagccgc agcattaccg
    27481 ccttgatggt ccgacgcgcc tgaaatttat tcaggatttg agtgagcgga aaacgcgtat
    27541 cgaatgggta cgccagttta tgcgtgaact ggaagagaac gcgatcgctt aatggtaaat
    27601 cgggggcgtt tctgcgcccc catctgcaac atttacaaat tctttgcact tccctgcact
    27661 atccgacact gtcaccatcc ataattcagg cttatctgtt tattacaata accttatatt
    27721 tattattgat ttttggtgat gatcaaaacg cgtttttctc gctggctaac gttttttacg
    27781 ttcgccgctg ccgtggcgct accggcaaaa gccaacacct agccgctgcc gccagcgagc
    27841 agtcgtctgg ttggcgaaaa caaatttcat gtggtggaaa atgacggtgg ttctctggaa
    27901 gccatcgcca aaaaatataa cgtcggcttt ctcgctctgt tacaggctaa ccccggcgtt
    27961 gatccttacg taccgcgcgc gggcagcgtg ttaacgatcc cgttgcaaac cctacttcca
    28021 gacgcgccgc gcgaaggcat tgtgatcaac attgcggagc tgcgtctcta ttactacccg
    28081 ccgggtaaaa attcggtaac cgtgtatcca ataggtattg gtcagttagg tggtgacacg
    28141 ctgacaccga caatggtgac caccgtttca gacaaacgtg caaacccaac ctggacgcca
    28201 acggcaaaca tccgcgcccg ttataaagca cagggaattg agttgcctgc ggtagtgccg
    28261 gctggaccgg ataacccaat gggccatcat gcgattcgtc tggcggccta tggcggcgtt
    28321 tatttgcttc acggtacgaa cgccgatttc ggcattggca tgcgggtaag ttctggctgt
    28381 attcgtctgc gggatgacga tatcaaaaca ctctttagcc aggtcacccc aggcaccaaa
    28441 gtgaatatca tcaacactcc gataaaagtc tctgccgaac caaacggtgc gcgtctggtt
    28501 gaagtacatc agccgctgtc agagaagatt gatgacgatc cgcagctgct gccaattacg
    28561 ctgaatagcg caatgcaatc atttaaagat gcagcacaaa ctgacgctga agtgatgcaa
    28621 catgtgatgg atgtccgttc cgggatgccg gtggatgtcc gccgtcatca agtgagccca
    28681 caaactctgt aaatcctgga taaaaaaaat ccccgcgaca cggcatgtcg cggggaaaaa
    28741 atcagcagta gcaggcatta atgagggtta atgcttattt ataaattact gctgttccat
    28801 ggagggtatt cggaccggtt acagaagtaa tacggaaaga ttttgcgccc atctcatccg
    28861 ctttttgcgc cagctgctct tccagcgatc ccagatttgt ccccgcgtta gcactgatag
    28921 taccgacttt ttgttggcct tctggcgttg actgaacttc gacagccgca aagttggcaa
    28981 atgacatgga gcttaaaatc gccgcagcga tgagggtttt tacgtttttc ataatagtgg
    29041 ccttatgcag atgaatgacg tcgcaagaaa ttacttaacg atcgataact aaatgataga
    29101 tgtgatctgg atcacataca agatattttt tataacaatc attaattaat ttaaaaatct
    29161 ttaaatttca gttaaataga aagcaattat ttttatggga tctggactgg tgaacggagg
    29221 tgattatttt tataataacc tttcattcac agtcaccagg ttcaacggca tcatggcaac
    29281 tgactcaaca caatgtgtaa aaaaaagccg tggccgccca aaagtgttcg acagggatgc
    29341 cgcgcttgat aaggccatga aattgttctg gcaacacggt tatgaagcga cttctcttgc
    29401 ggacctcgtc gaagcgaccg gagccaaagc gcccacgcta tacgcggaat ttaccaacaa
    29461 agaggggtta tttcgtgccg ttctcgaccg ctatatcgat cgttttgccg ctaagcatga
    29521 agcacagctg ttttgtgaag agaaaagcgt ggagtctgcg ctggctgact attttgctgc
    29581 catcgccaac tgctttacca gcaaagacac cccggcaggc tgcttcatga tcaacaactg
    29641 caccaccctc tccccagatt caggagatat cgccaatacg ttgaaatcac gccatgcgat
    29701 gcaagagcgc actttgcagc agtttttatg tcaacgacaa gcgcgcgggg aaatcccgac
    29761 ccactgtgac gtgacacatc tggcagaatt ccttaattgt attattcagg ggatgtcgat
    29821 cagcgcacgc gaaggtgcat cgctggaaaa actgatgcag attgccggaa cgactttgcg
    29881 tttatggccc gaactggtga aataagtcgc aaaaaaagcc cctcagcctg agcgaatgag
    29941 gggccaaatt gcagagtaca gccttgttat actttgttat tcaaaatcag ttgcccgtta
    30001 ctatctagcg ggatctgcgt acccggatcg cggtccatgc ggattttgcc ctgctgatcg
    30061 ccaatcttat aggtcacatc ataaccgagc attttttctg acttgtcata caccgtttta
    30121 caacgctgtt gcgtagtcgt gtaagtatcg ctttcctgga gagagccctg gatctggtta
    30181 ccggcatatc caccacccag cgcccccaca acagtggcga catctttacc acgaccacca
    30241 ccaaactgat gccctatcac gccgccagca acagcgccga gcaccgaccc ggtaatgcga
    30301 ttttcatcct gcaccggtcg acgatgggtc actgtgacgt tgcgacactc ctgacgcggt
    30361 gttttaaccg tttccttgat tggggttgca gaaacaacct gagcgtattg cgggccccgt
    30421 tcaaacacgt tcagactggc cactgccgct acgcccagcg cagctgcgac accaatcccg
    30481 atacccgcca acattgattt attcacggga acacctcctt ctttgcagtt atgccaatca
    30541 tgcaagcgaa ggagaaaagc agctatcaga cagaagatga aaagcgcggg aatggctgga
    30601 aatacttaag gattcggaga agtctgaagg aatgcctgat gcgacgctta acgcgtctta
    30661 tcaggcctac cgctcgtacc atttgtccag ggcggatgag gcgtttatgc cacatccgcc
    30721 agtgtacgtc gattaatgca acttcaaacg cggacggata acgcggttaa tactccccac
    30781 cagcatcatt aatccggttt taaagtaacc atgcagcgca atctgatgca ttcggtatag
    30841 cgagatatat acaaagcgcg caattcgtcc ttcaatcatc attgagccgc gcgtcaggtt
    30901 acccatcagg ctaccgacag tggagaagtt cgacagcgat accagcgaac catgatcttt
    30961 atactgataa tttttcagcg gtttaccgtt catctgcgcc agaatgttgt tcattgcgca
    31021 ggtcgccatc tggtgtgcag cctgagcacg cggcggaaca aagccccctt ccggacgcgg
    31081 gcatgacgcg cagtcgccaa tagcgtaaat gtctggatcg cgggtggttt gcagcgtcgg
    31141 ttccaccacc agctggttga tacggttggt ttcaagacca ccgatatctt tcaggaagtc
    31201 tggcgctttg atcccggctg cccacaccat cagatcagcc tcaatatatt caccatcttt
    31261 agtgtgcagg ccgccttcat cagcactggt gaccatggtt tgcgtcagca cgcgaacgcc
    31321 aagtttcgtt agctcgttgt gggccgcagc agagatacgt ggcggtaatg caggcaaaat
    31381 acgttctccc gcttctacca gcgttacgtt cagggcttcg ttggtcaggc ctttgtaacc
    31441 gtagctgtgc agttgcttga ccgcattgtg caattcagcg gagagttcta cacccgtcgc
    31501 gccgccgccg acaatcgcaa tgttcacttt accattcgcg cccaggttgg cggagtattt
    31561 caggaacaga ttcagcatct cctggtggaa gcgacgcgcc tggtgcgggt tatcgaggaa
    31621 aatgcagttc tctttgacac ctggcgtatt gaaatcgtta gaggtgctac ccagcgccat
    31681 taccagggtg tcataggcga ttttacgttc cggaaccagc agttcacctt tctcatcgcg
    31741 cagttctgca atagtgattg ttttcgcttc acgatcaata tcaatgacgg aacccagctg
    31801 gaactggaaa ccatgattgc gcgcatgggc cagatagctc aacgcatcga cgccttcatc
    31861 aagcgagcca gtcgccactt cgtgcagcag cggtttccac agatggctgt ggttacgatc
    31921 gaccagcgta attttggctt ttttcttgcg tcccagctta tgccccagct gtgttgccat
    31981 ttccagccca ccagcaccgc cgccgacaat cacaattttt ttcaatggcg tagtcaacgt
    32041 gaccccctta aatttattaa ccaattgtta attaatggtt ataaacatag ctcttaatta
    32101 acaacaggtt acagcaatga aaatgttctt cgctattgag aataacatga atggtgcatt
    32161 ggtcatacca aaattgatgt taatcaagtt ttgttgctga aaagataggc gactttaccg
    32221 ggtgagatca aaaaacagag tttattgttt attatttgag caagtccgga agggtgatat
    32281 agggtgacgc gtatatcgag ggcgtttggt cgtcctgata agatgcgcag catcgcatca
    32341 ggcattgatg ctcgactgtc ggatgcggcg taaacgcctt atccgactta cagaattcgg
    32401 agcgcatcag gtaccggcaa ggttacctga tgcgacgagg gcatttaccc gagggttttg
    32461 aacgccttaa tgcgctgcaa atgcggggag atattcttga atttgtgcgt ctgctcttcg
    32521 tcccagacaa tctcgtaata atgatgcaac tcttcagatg tccgctggct gttaagcgct
    32581 tcatcattac gcgacaaaat caccaggcaa cgatcgcgat tcttctcgcg gaagttggtc
    32641 acacacttag tggcaatatc ggcatactct tccgggcgat caatcttgcc ttccatgttc
    32701 tcataaggga acaaattagg gttgaagatc acctggcgga tatcgcaaag aaaaccaatc
    32761 cgttccgccc agtatccgcc cagaccaacg ccgcaaatca gcggacgctc gtcgacgttc
    32821 agttgcaaca ttttgtccac ttctttgagc agatgctgca tatcatgttt cggatgccgc
    32881 gtactgtagc ttatcaagcg tacatccggg tcaataaact gcaattgtaa gactttctcg
    32941 tggttacccg gactgttaga gtcaaaaccg tgtaaataga taatcatcgc ttcctcacat
    33001 aagccagggt tagtgacctg ctttctcttc ctgccagcgt tcatgtaact gattcagacg
    33061 ggtgctgatc gccttccagc gagccgagtc catcagttcc tgtcgcgaaa atgaaccttt
    33121 atgatacaaa cgtgtaacac gttctgcctt gatcggtgac agattatcta acacgctgac
    33181 ggccccttta cgattattgc agaccaggat catatcgcaa cctgcgtcca gtgatgcctg
    33241 cccgcgttcg gcataactgc ccataatcgc ggcaccttcc atcgataaat cgtcagagaa
    33301 aattacaccg tcaaaacaca gttcctgacg caaaacggtt ttcagccagt aggaagaacc
    33361 gctcgccgga cgcggatcaa catcactgta gatcacatgc gcaggcataa tggcgtcgag
    33421 tttattttcg cggattaagg aactgaagac cgacatatct ttagcgcgaa tctccgcttg
    33481 tggacgtgga tcgcacggtg tttctttgtg tgagtctgcc gttactgcac cgtgtcctgg
    33541 gaagtgtttc ccggtcgttt tcattccggc ttcatgcata ccatcaataa accggctggc
    33601 aattgccagg gctttttgtg gatcggcatg ataagaacgc tcgccaatcg ccgcgctgat
    33661 atgcccgaca tccagcactg gcgcaaagct gatatcaata tccatagcga tcatttcgct
    33721 ggccatcagc caacccgcct cttgcgccag tttgccgccc tcttccattc ccaacagcgc
    33781 agcaaaggat tgtgctgccg gtaagcgggt aaaaccttcg cgaaaacgct gcacgcgtcc
    33841 accttcctga tctaccgcca ccaccagatg attgcgcgat gctgcgcgga tctggcgcac
    33901 cagttcacgt aactgggcag gatcatgata gttacgcgta aagagaatca gccctcccac
    33961 cagcggatgc gccagtattt cacgctcttc cgcgtccagt tcgtaacctt cgacatccaa
    34021 cattactgga cccacactgc tctccttatt gttttattaa tagctgccgc caggtgtcat
    34081 cggccagcct gataaattgt tgatcgccga tttgtcgcca gcggtactca aaccaccctg
    34141 ctttgagcat cagcagccag ggaaaccatc gcctgacctg acgccataat tgcgccggat
    34201 aaatcttcgc gcgagtggca tagtcattga ccaattgccg gtgctgttca gtattttcca
    34261 cccacaccgc cgccagttcc agcgcgatat caccatctcc ggcatactcc cagtcgatga
    34321 gttttaaccc tgacgcgcta tgcactaaat ttccggcgtg gacatccata tgcaatggac
    34381 ttaagcgtaa aggccgtggt tcccgcgctt tgcgcagacg ttttaacatt cgcagccaac
    34441 ccacagtccg ccgcgccgga tcgctttgct gccagtacag ttccagtaag ggcaacagcg
    34501 ttattcgcca gccaaaacgt ggttgttgat gaagataata cagcaagcct gccagttcgt
    34561 tggtatccgg caaatacgtt tttaccgcgc cgggcagata gtcgactacc atccagtcac
    34621 ggagatataa atgcggcttc ggtgcaatgc ttgcgggtag ttgtgataaa gcccgatact
    34681 ggcgcaagaa cgcggactgc ggcgcatcag gatcgtgcgg ctgacgcaca acaaaacgct
    34741 ggccctgatg ttcaatgaga aaactaccgc cactaagccc actattaaac gtcgtgacgg
    34801 gatgaaactg cgggaaaaag cgcgacagca attcgtcgcg cgtgatggga ttattgctgc
    34861 gaaacggcac ctttacctga ccagataatt tcgcccgtct gcaccagcat cagctgcatt
    34921 tgtagggtcg gagcgttaac gttgccagag gcgctggagt acagcacgta atgagcgccg
    34981 acattgcggg caatgcctat ggctttacta cgggtaccta aactgtcctg cggcgacaaa
    35041 cctaactgtt gcttagccat cgacagctgc tgggcggaaa ccagggtaaa tttcccgtta
    35101 ttagccagcg catttcgcag cgtttcggtc gcttctgcgg cattcagcga accgttagta
    35161 cggttgttaa cgctatcaac cagcaggacg ctacccgcag tcaccccgtc agccccaagc
    35221 atcttactga ccatcggctg cattgcgcca ttccagtcat aatggcgaat atgcggcgca
    35281 ggcggtgccg tttgatcttc gtgctcaatt gggcctggct gctgcgggat cgtcggcacc
    35341 gagggcactg tggggacagg ctgttgtggc tcggctggtt gttccggcgc tggtttcact
    35401 tcttctaccg gtgcaggttc acgttgcccc acacacccgg cgagaaacat cgccagcgcg
    35461 gtaatcaagg cgtagcgact catttttgtc atcaagattc accccttaca aatatagata
    35521 aagtctgact ttgtgcgccc ccagaaaatt ggcgctgccg tacagcgtta ccgccgaatg
    35581 tgcgggaatg ctaacgctgc gtggcctttc cagaggatgc atctccagcc ctctggcgtc
    35641 ataccagtaa aaacgataat gaacggtaac gggttcttgc ctttcgttat aaagcgttga
    35701 ggatgctgaa ggttgaatat cagacgtcga aaggacgggc ttttctgcac tgatgcccgc
    35761 agccaataaa gatgactcca tcaccagcga ttgctcatca ttcaccggaa tttccggatg
    35821 tgaacgacag cccaccagca gtaacaacgc cagagacacc agcccaaagc atccttttct
    35881 catcgttaca gacctttatg cgccagcatt ggtcccagcg gacggccacc caacaagtgc
    35941 atatggatgt ggtaaacctc ttgtccgcca tggcggttgg tattcatgat cagacgatag
    36001 ccatcttcgg caataccttc ttgctcagca atttttgccg ctacggtgat catgcgtccc
    36061 agcgcctgct catgctcagc tgacacgtcg ttcacagtcg gaatgaggat attcggaatg
    36121 atcagaatat gcgttggcgc ttgcggcgaa atatcgcgaa acgccgttac cagatcatcc
    36181 tggtagacga tatcggaggg gatctcacga cgaataattt tgctgaatat agtttcttct
    36241 gccacgacat tttccttttt caaatagcca tgcgttgcgc catgctacgc cgcgccacac
    36301 tgcgagtata gagtatggtc gagttaaacc tgctctttca accttaactc acgatttctt
    36361 aagcaaaaaa tcgttttgct gccgtatata tcgccattat tcccatttct gcctgcgaat
    36421 gcgctgaaaa gacattctct gtttggttta cacgacaaat atgaatgcgc atatttctca
    36481 tttgcattta caaacaaaat tattcgcaca ctaaatataa ccgctcatca ccctcctccg
    36541 gcatgagcct gacgacaaca taaaccaaga gatttcaaat gctttcaaca caatttaaca
    36601 gggataatca atatcaagcc atcaccaaac cgtcactact tgccggttgc atagcactgg
    36661 cactattacc ttctgccgct tttgctgcac cagccactga agaaacggtg attgttgagg
    36721 gttcagccac agctccagat gatggcgaaa atgattacag cgtaacgtct acctctgcgg
    36781 gtaccaaaat gcagatgact caacttgata ttcctcagtc ggtcactatt gttagccagc
    36841 agcggatgga agatcagcag ttacaaacgc tgggcgaagt gatggaaaac acgctgggga
    36901 tcagcaaaag tcaggcggat tccgatcgtg ctctttatta ttcccgtgga ttccagatcg
    36961 ataactatat ggttgatggt atccccacct attttgaatc gcgctggaat ctgggcgacg
    37021 cactttctga tatggcactc tttgaacgcg tagaagtagt gcgtggcgcg acaggactca
    37081 tgaccgggac gggtaatcca tctgcggcaa ttaatatggt tcgaaaacac gcgaccagtc
    37141 gtgaatttaa aggcgatgtc tcggcggaat acggtagctg gaacaaagaa cggtatgtgg
    37201 cggatttaca aagcccactc accgaagacg gtaaaatccg cgcgcgaatt gtcggcggct
    37261 accagaataa cgactcatgg ctggaccgct acaacagtga aaagaccttc ttctcgggca
    37321 ttgtcgatgc tgatttaggc gatcttacga cgctttcagc cggttacgaa tatcagcgca
    37381 ttgatgttaa tagcccaacc tggggcggtt taccgcgctg gaatactgat ggcagcagca
    37441 acagttacga tcgtgcacgc agtaccgcac ctgactgggc gtacaacgat aaagagatca
    37501 acaaggtctt tatgaccctg aagcagcggt ttgctgatac ctggcaagcg acactgaatg
    37561 ccacccactc tgaagtcgaa tttgacagca aaatgatgta tgtcgatgcc tatgtaaaca
    37621 aagcggatgg tatgctggtt gggccataca gtaattatgg acctggcttt gattatgtcg
    37681 gcggcaccgg ttggaacagt ggcaaacgta aagttgatgc gctggatttg ttcgctgacg
    37741 gtagttatga attgtttggt cgtcagcaca atctaatgtt tggtggcagt tacagcaaac
    37801 aaaacaatcg ttacttcagt tcatgggcca acatcttccc ggatgaaatt ggcagtttct
    37861 acaactttaa tggcaatttc ccacaaaccg actggtcacc acagagcctg gcacaggacg
    37921 ataccacaca tatgaaatcg ttatatgctg ctactcgagt cacccttgcc gatccgctgc
    37981 atctgatcct cggcgcacgt tataccaact ggcgggttga tacgctgact tacagcatgg
    38041 agaaaaacca caccacgcct tacgctggtc tggtgtttga catcaatgac aactggtcga
    38101 cctacgccag ctatacctct attttccagc cgcaaaatga tcgtgacagt tcaggcaaat
    38161 atctggctcc aatcaccggt aacaactacg agctgggtct gaaatcggac tggatgaata
    38221 gccgtctgac caccacgtta gccatcttcc gtattgagca ggataatgtc gctcagtcca
    38281 ccggtacacc tatccccggc agcaacggcg aaaccgccta taaagcggtg gatgggacag
    38341 tcagtaaagg ggtggaattt gaactcaacg gcgcaattac cgacaactgg cagctgacat
    38401 ttggcgcaac gcgctatatt gcagaggata acgaaggaaa cgccgttaat cctaatctgc
    38461 cacgcaccac ggttaaaatg ttcaccagct atcggttgcc tgtcatgcca gagttgacag
    38521 tcggcggtgg tgttaactgg caaaatcgcg tgtataccga caccgtgaca ccgtatggca
    38581 ccttccgcgc cgagcaaggt agctacgcgc tggtggatct cttcacccgc taccaggtga
    38641 cgaaaaactt ctcgttacag gggaacgtca ataacctgtt cgacaaaacc tacgatacca
    38701 acatggaagg ttctatcgtc tacggcgcac cgcgtaattt cagcattacc ggcacgtatc
    38761 aattctgaat aacacctgta aaaaaggcag ccatctggcg gccttagtct ccccaacgtc
    38821 ttacggatta gtggttacgg atgtactcat ccatctcggt tttcaggtta tcggatttag
    38881 taccgaaaat cgcctgaaca ccagaaccag caaccactac gcccgctgcg cccagtttct
    38941 tcaggccagc ctgatccact ttagacacat cagcaacgct gacgcgcaga cgggtaatac
    39001 atgcgtcgag gttagtaatg ttttctttac caccaaatgc agcaaccaga gccggtgcca
    39061 tttcgctggt acctgttgct tttgcatctt cagtcgcgtc ttcacgaccc ggcgttttca
    39121 gatccagtgc tttaatcagc acgcggaaga tggtgtagta aacaatcgca taaccgatac
    39181 cgacgatcgg gaacagccac agtttgctgc tgttaccaga cagaacgata aagtcgatca
    39241 gaccgtgcga gaacgacgta ccgtcacgca tccccagaag aatacagatt gggaatgcca
    39301 ggcctgccag aatcgcgtgg atgatgtaca ggatcggcgc aacgaacatg aaggagaact
    39361 cgatcggctc ggtgataccg gtcaggaacg aggtcagcgc cgcggagatc ataataccgc
    39421 ccactttcgc gcggttttct ggtttagcag agtgccagat agcaattgcg gcagctggca
    39481 gaccgtacat tttgaacagg aagccaccag acagtttacc cgcagtcggg tcacccgcca
    39541 tataacgcgg aatgtcgccg tggaaaacct gacctgctgc gttggtgtat tcaccaatct
    39601 gcatctggaa aggtacgttc cagatgtggt gcagaccaaa cggcaccagg caacgttcga
    39661 tgaaaccgta aatgccaaac gcaactaccg ggttctggta agcagcccac tgagagaagg
    39721 tctggattgc agaaccaatc ggcggccaaa tgaaggacag cacaacgcca gtaaagatgg
    39781 cagccaggcc agaaatgatc ggcacaaagc gtttaccggc aaagaagcca agatactcag
    39841 gcagcttaat acggtagaaa cggttaaaca tgtacgctgc gatcgcaccg gagataatcc
    39901 ctccgagtac gccagtatcc gctaggtgtt tagaggcgat ttcttcagca ggtaaatgca
    39961 gtaccagtgg cgcaaccacg gccatggttt taaccatgat gccataggca acaactgcgg
    40021 ccagcgcgga tacgccatcg ttattggtaa agccgagggc gacaccgatc gcaaaaatca
    40081 gtggcatgtt tgcaaagacg gaaccgcctg cttctgccat aacatgcgat acaacggcgg
    40141 gcagccagct gaaattcgcg gaaccgacgc ccagcagaat acctgcgata ggcagtacgg
    40201 ataccggcag catcagcgat ttaccgacct tttgcaggtt agcaaatgca ttcttaaaca
    40261 taattgagag tgctcctgag tatgggtgct ttttttacgt tctcacgcgt ggcaaggggg
    40321 gagagcctcg ccgtgtacag ggcatctaag cgccctttat ttattacaca gagtaaaata
    40381 attcagtgcc gatttgtttg acggctatca cgtttcaact tcagatcatt ggagaagttg
    40441 cacattttga caaaacatat gtcacaatat ttcaaacgtg gttattcacc gcccaatttt
    40501 cggcggtgaa ctttactcgt tttagccatt aattacgagc tttaaaaaaa ctcattcaac
    40561 ggatggattg aaggcgggaa gcgtcgatgt gaaacagacg ggcgaagtta tcggtggtta
    40621 cctgcgccag ttcttcaacg gcaacacctt tcaacacagc catgtattct gcaacgtcac
    40681 gaaccatcgc aggttgattc tcttttcctc gatgcggtac cggcgcaagg taaggtgagt
    40741 cagtttccac cagtaaccga tccaggggga cataacgcgc agcatctcga agttgctccg
    40801 cattacggaa ggtcacaatg ccggaaaagg agatgtaaaa tccgagatcc agtaatttac
    40861 ccgccgtttc tctgtcctct gtaaaacagt gtagtacgcc accgcaatcc gtcacttttt
    40921 cttcgcgcag aattgccagc gtatcggcgc gggcgtcacg ggtatggacg ataaccggct
    40981 tgttcagttc acgaccaatc tggatatgat ggatgaagga ctcttgctga cgaactttag
    41041 tttccggtgt gtaataataa tccagcccgg tttcacccag cgctacaaca ccctcttctg
    41101 ccgccagacg gcgtaaatct tctacatcgt agggatcatt ctggttgagt ggatgcacgc
    41161 cacaagaaaa tacgacgttg tcacgttcgc ctaccagatc ccgcatatgt aaataacccg
    41221 gtaatgttgt ggcgactgcc agacaaaatt tcacatcgcg tgcggcggct ttcgccagaa
    41281 cgtcatccac gtccttatgc aaagattcat aatccagacc atcgagatgg cagtgtgagt
    41341 cgactaaaaa catgatgtct ctcttaaaga tgaggaaccg gtagcacaac gcccggttgc
    41401 aggtaatgct caatacgcag taaaagatcg gtgatgagaa gctcgcggtt gatgcctgtg
    41461 acagacatta actgttcacg aatgtggcaa acatccccca gtatagcctg caggcgcgag
    41521 ggagaaagat ggtttgccag ttcgacgaca aggcccggca catcaacatt ggtcacctgc
    41581 gcagcaccat gatggcgttt tagcgcatcc atcagcaacg ttgccagcca gtgtaaacgc
    41641 gccggagctt gttcatgatt aagggccgcc agcagcgaat accagtcgcc cgatggcacg
    41701 ctatatgcca atgcctgaca caatgtttca cgagcctgcc agttatctcc ctgaaacaac
    41761 gccagtgccg cgccaggcga accggcgctt aagcgcaatg cggcaagtaa tgcatcctgt
    41821 gacattgtca cttcgcgtga aagccaggtc acggcgtact gttccggcgg cggcgcaagg
    41881 taatgtaacc gacaacgact acgtaatgtt gccagtaaac gttcaggctc gcgggtagcc
    41941 aggaaaaacc aggtttctgc tggtggctct tcaagcgttt tcagcaatgc gttagccgcg
    42001 gcgtcggtca gtaaggcagc atcggttacc cagacaactt tcgcaccacc taggcgtgcg
    42061 tgctcattca gcttttcggt gacctcacgt accgcatcaa tgcccagcgt atttttccct
    42121 ttttcgggag ccagggtgta gtaatcggga tgcgtgccag cctgcatcaa ctgacatcca
    42181 cgacagtgac cgcaactttt gtggccctgc ggttgttggc agagtaaata acggctcagg
    42241 gcgtagatta aagcatcatc gcccatgccc ggtaacgcct gaatgagtag cgcatggtga
    42301 cctcttccgg cctgatagct ggctaccagt ttttcgaaat caggtcgtaa ccatggatac
    42361 catctcatgc gtccaactcc ttcacccagt gggtcacggt agtgcggatt gcatccatca
    42421 cggcctccag cggctgggtg gcatcaatgg tatgaatact tttatcttgt gcagccagtt
    42481 ccagatagcg ggcgcgggtg cgattaaaga aatcgaaaga ctcttgctca atacgatcca
    42541 gctcgccgcg cgcacgcgcg cgttttaagc caacttccgg ggtaacatcg agatagagcg
    42601 ttaagtcggg gcgaaaatcc ccgagaacag catcacgcag tgttgccagc atatgttggt
    42661 caataccacg tcctccgccc tgatacgcct gagtggagag atcgtggcga tcgccaatca
    42721 cccaggtgcc gttagccaga gctggtttga tgaccgtttc taccagttga acgcgcgcgg
    42781 cataaaacat cagaacttcg gctttatcgg taatgacttc atcgcctacc gatttgatat
    42841 ccagcaccag gcttcttaac ttttcggcaa gttgcgtacc gccaggttcc cgagtgaaaa
    42901 ccatgtcgcg gatacccagt tgctcaagcg tctcaaccac cacattacgc gcggtagttt
    42961 tgcctgcgcc ttccagcccc tcaatgacga tatacttact gcgcattttt ttccttaagc
    43021 actttcagat aatcctgcac agacttgtta tggctggcaa gattggtatt aaacgtgtga
    43081 ccacctttac catcggccac aaaatagaga tacggcgttt ttgccggatg cgcagcagcc
    43141 ttcagcgaat ccgcccccgg cgtagctatc gcacccggcg gcaaaccggt aatggtatag
    43201 gtgttatacg ctgtcggcgt ttccaggtct gcacgagaaa gtttgccatt ataacgctct
    43261 cccatcccgt aaatcacggt tgggtcggtc tgcaggcgca taccaatgcg taaacggttg
    43321 ataaataccg aggcaacctg atcgcgttca ctggcaacgg cggtttcttt ttcgataatt
    43381 gatgccatcg tcaccagctg atttttatct ttataaggca gaccgtccgc acgcccttcc
    43441 caggcgctat cgaccgcttt caccattttc ttgtgcgctc gcttgagtaa cgcgacatcg
    43501 gtggtattgg cggtatacat ccaggtgtct ggccagaacc aaccttcaat ccactccggg
    43561 ttttccagtt caagtgcctg cgctacggtg gcgtacttat cgtcactcag cgtgtgcttg
    43621 atatacggag cctcacgcaa ttgcttgagg tagtcgctca gacgcatccc ttctaccagt
    43681 cgcagcggga actgtgcttc tttaccgctt tccagcaatt tcagcatctc gcgcacggtc
    43741 atctgcggtg taaagcggta agtcccggct ttaaagtgag aaagatccgg ttcgatacgc
    43801 agcaaccatt gaaaaacccg tgggcgattg atgatcttat cggcataaag ctgttcacca
    43861 agcgccagac gtccggtccc tggcttcagg gtaaatatcg tctcttcttt gataagcaat
    43921 ttgctgtcgg caagatggcg aaccttccag acgcccacac cagcggcgat acccagtacc
    43981 accagcaata acaagataat caataacact tttttcatga ctaattcggg cgctcacaaa
    44041 gtggggctaa atattcatat aacgttgctg acgaaaagga gacatcgcca caggcacgta
    44101 cgggcatcac tggcattaac gcattacaaa taaccatctc atctgcctgc aacgcctctt
    44161 ccagagaggc ttgcacttcg acaagctgat aagaggattg tgccagcaaa cggatacaga
    44221 attgtcgcat aatgccgtta acacctgcct gatccagacg cggcgtataa actacattgc
    44281 ccttccgcca gaacaaatta gccgcacagc attccgtaac ccacccttcg ctgtcaagga
    44341 ccagcgcctc atcagcgttt gtctgctcaa gatgagagcg gatcaatact tgctcaagcc
    44401 gattaagatg tttaatacct gcaagatgag gattgcgccc cagccgcacc gggcttagcg
    44461 ccaacgtcat cccttcgtta cgcaaacggt cgtaatgtgc aggataaacc gtaacggaga
    44521 gaatccgcgt tgccggtcca ctgttcaatg tgctgtaccc tcgcccgcca ctaccgcgac
    44581 tgatcacgac tttcagcaca ccattttgct gttctgctgc cagcgttttc atctcttgtt
    44641 caagctgagg ccagaagtca cagaaaatca tcaaccgctg acaagcatcc tgtagtcgct
    44701 ggatatgcgc cgataacaaa ctgactttac cgtcgataac tctggcggtg gtaaaacaac
    44761 catcaccaaa ctgcgttgcc cgatcgctta ctgccagcga ttcctgctta tgaccgttaa
    44821 ttaagaacat accggctcct tatcagagaa ttgttagtgt ggcagcatgt tcactacgga
    44881 acaagtcgga ataaaagcta agaaaaaagg cccgcaagcg gaccttttat atgggtggga
    44941 aatgacaact tagatctttt taaagatcaa agaaccatta gtgccaccga agccgaagga
    45001 gttacacagc gtgtattcca ttccgctgac ctgacgtgct tcatgcggta cgaaatccag
    45061 atcgcaacct tcatccgggt tatccaggtt gatggttggc ggaacagcct gatcgcgcag
    45121 cgccaggatg gagtagatag attctactgc acccgccgca cctaacaggt gaccggtcat
    45181 agatttcgtg gagcttacca acacacggct tgcagcttca ccgaagatgg ttttcaccgc
    45241 ctgcgcttca gctttatcgc cagccggcgt agaagtaccg tgcgcgttaa cgtagccaat
    45301 ctgactcgct tcaatgcctg catcacgcag agcatttgcc atcgccagag ctgcgcctgc
    45361 gccattttct ggcggtgacg tcatatgata agcatcgctg ctcataccaa agccgacgag
    45421 ttcagcgtaa attttcgcac cgcgtttttt cgcgtgttcg tactcttcaa gtaccagcat
    45481 accggcacca tcgcccagta cgaagccatc acgctcttta tcccacgggc ggctcgccgc
    45541 ttgcgggtta tcattgcggg tagataatgc acgtgccgcg ccaaaaccac caacacccag
    45601 cggcgtactg gctttctctg cgccacctgc aaccatcacg tcagcatcgc catacgcgat
    45661 aatacgcgca gcatggccaa tgttgtgcac gccggaagta caggcggtcg cgatagagat
    45721 gcttgggcca cgcaggccat acatgatagt cagatgacct gccaccatgt tcacaatcgt
    45781 tgacggaacg aagaatgggc tgatcttacg tggaccaccg ttcatcagag atgtgtggtt
    45841 ttcttcgatc agtccgaggc cgccaatccc ggagccaatt gcggcaccaa tgcgggttgc
    45901 gttctcttcc gttatttcaa ggccagaatc ctgcatggcc tgaacgccag cgacaattcc
    45961 atattgaatg aaggcatcca tcttgcgctg ttctttgcgc gagataatgt cctcacagtt
    46021 aaaatccttt actaagccag caaatttcgt tgcataggcg ctagtatcga aatggtcgat
    46081 taggctgatg ccactctgac cggcaagcag agctttccag gtagactcta cggtattgcc
    46141 gacaggagac aacatgccca gtccggtcac aactacacga cgcttagaca cgtttgtcct
    46201 ccagggaggg aaaaaatgat tctagtggga caaaaagata aaactcaggc ggtcgaacga
    46261 ccgcctggag atgttcactt acgcctggtg gccgttgatg taatcaatgg cagcctgaac
    46321 ggtggtgatt ttctcagctt cttcgtccgg aatctcagta tcaaactctt cttccagagc
    46381 cattaccagc tcaacggtgt caagagaatc cgcgcccagg tcttcaacga aagaagcatt
    46441 gttggtaact tcttcctgct taacgcccag ctgttcgccg ataattttct taacgcgttc
    46501 ttcgatagtg ctcatactct taaatttcct atcaaaactc gctttcgcga tggttttcgt
    46561 agtgtataaa atgttgaaaa atttgcaact aaatcccggc aggtcttacc acgattttac
    46621 gttattttga ggccttaccc cccaataacg caaatcattt tgcactaatt attactcata
    46681 accacgcaga ctgcgcgcaa catgagcatt ttgtgcaaat cgcggtcaga ccatgtacat
    46741 cccgccgttc acatgcaaag tttcacccgt gatgtaagct gcttcgtcgg atgccaggaa
    46801 tgcaaccgcg ttggcgattt cctgtgcgcc gccgaggcga cccgcaggaa cctgcgccag
    46861 gatacccgca cgctggtcat cgctcagcgc acgtgtcatg tccgtttcaa taaagcccgg
    46921 agcaacaacg tttacagtaa taccgcgtga cgcaacttcg cgcgccagtg atttactgaa
    46981 gccgatcaag cccgctttcg ccgcagcgta gttggcctga ccgccatttc ccatggtacc
    47041 aaccacagaa ccgatagtga taatacgacc atgacgcttt ttcatcatag cgcgcattac
    47101 cgcttttgac agacggaaaa cagatgaaag gttggtttcg ataatatcgt tccactcttc
    47161 atctttcatt cgcattaaca ggttatcacg agtgataccg gcattattga ccaggatatc
    47221 cacttcacca aattctgcgc gaattttttc cagaacagat tcgatagatg ccgggtcggt
    47281 cacattcaac atcagacctt tgccgttggc acctaaataa tcgctgatcg cctgagcgcc
    47341 attttcactg gtcgcagtgc caataacttt cgcgccacgg gctgcgagcg tttcagcaat
    47401 tgcgcggcca attccgcggc ttgcaccggt taccagtgcg atttttcctt caaaattcat
    47461 gattttcctc ttttaaagct caagcgccgc tgccatcgct gaaggttcgt tcagcgccga
    47521 ggcggtcagg gtgtcgacaa tgcgtttcgt caggccagta agcaccttgc ccgggccgac
    47581 ttcatagaga tgttctacgc cttgcgctgc catgtactca acagacttcg tccactgaac
    47641 cgggttatac aactgacgta ccagtgcgtc acggatggca tcaccattgg tttcgcattt
    47701 cacatcaacg ttattcacaa cagaaactgt tggtgcgtta aaggtgattt tcgctaattc
    47761 tactgccagt ttgtcggctg ctggtttcat cagcgcacag tgagacggta cgctcactgg
    47821 taacggcaga gcacgttttg cgcccgcagc tttacaggca gcgccagcac gctcaaccgc
    47881 ttctttatga ccggcaataa ccacctgtcc cggagagtta aagtttaccg gagaaacgac
    47941 ctgacctgct gcagcttctt cacacgcttt cgcaatagac gcatcatcca gaccgatgat
    48001 tgccgccata gcgcccgtac cttccggtac ggcttcttgc atgagcttgc cgcgcatctc
    48061 aaccagacgc accgcatcag cgaaatcaat cacaccagcg caaaccagcg cggaatattc
    48121 ccccaggctg tgaccggcca tcattgccgg tgctttaccg ccctgctgct gccatacgcg
    48181 gtacagcgca acagaggcag tcaacagcgc tggctgggtt tgccaggttt tattcagttc
    48241 ttcagctggc ccctgctggg tcagcgccca caggtcgtag cccagcgccg cagaagcttc
    48301 agcaaacgtt tcttcgacaa ttggatagct cgccgccata tcagccagca ttccaacggt
    48361 ttgagaaccc tgtccaggga acacaaatgc aaattgcgtc atgttttaat ccttatccta
    48421 gaaacgaacc agcgcggagc cccaggtgaa tccaccgcca aaggcttcaa gcagaaccaa
    48481 ctgccccggc ttaatgcgcc cgtcgcgtac agcttcatcc agcgcgcacg ggacagaggc
    48541 cgcagaggta ttaccgtggc gatccagcgt caccacgaca ttgtccatag acataccgag
    48601 ttttttcgcc gttgcactga taatacgcag gttagcctga tgcggaacca gccagtccag
    48661 ttgagaacgg tcaagattat ttgccgccag cgtctcatca acgatgtgcg ccagttccgt
    48721 taccgcaacc ttgaagactt cgttgcccgc catcgtcaga tgaattgaat tctctggatt
    48781 cacacggtca gcattaggca gcgtcagcaa ctcaccatag ctaccgtcgg catgcagatg
    48841 ggtggagatg attcccggct cttcagaggc agccagcacc gcagcgcccg cgccatcgcc
    48901 aaaaataata atagtcccac gatcggttgg atcgcaggtg cgcgccagta catcggaacc
    48961 gacgaccaga gcatacttca ccgccccaga tttcacgtat tgatcggcta cgcttaatgc
    49021 ataggtgaaa cctgcgcagg ctgctgcaac gtcaaatgcc gggcaacctt taatgcccag
    49081 catgctttga atctgacaag ctgcgctcgg gaaagcgtgc gtagcagaag tcgttgccac
    49141 aacgatcagg ccaatctggt ctttctcaat gcccgccatc tcaattgcgc gtgtcgccgc
    49201 ttcaaagccc atggttgaaa cggtttcgtt tggcgcggca atgtggcgtt cgcggatacc
    49261 ggtacgagtg acaatccact cgtcagaggt gtccaccatt ttttccaaat cggcgtttgt
    49321 ccgcacttgt tcgggcagat agctgccagt accaataatc ttcgtataca tgtacgctca
    49381 gtcacttttc ggttatatac cgtcacttgc aaactgcgag ttcgctggca gcgtcctgct
    49441 accgcagagt tccgcttttg ccaccgtcca gcagctcaaa accagctggg tatacagatt
    49501 ccaggcgagc ggcaattcgc tgaggaactt gtcgctgcac cgcctgcact gcctgttcaa
    49561 tcgcgaccgc aaaagctcgc tgattggctg caccatgact ttttatcacc gtgccgcgca
    49621 atcctaacag acaggcgccg ttatactggt cggggttgag gtgactgaat cgcctcgtca
    49681 ggctcttttg tagccaacgc tttaataaca gtagccacca cgaccgtttt ttcccttcac
    49741 cctgagattt cagcagagaa aggaacatcc tgacaacacc ttccatcgtc tttaatgtga
    49801 catttcctgt aaagccgtca caaaccagca catctgtctt gccagttaac aactcattgg
    49861 cttcaagata gccgatataa ttgatagaag ggattgtttt aagcaccgct gaggcatccc
    49921 gaatactgtc gagacccttt acttcttctt caccaatatt gagcaacgcc acgcgaggat
    49981 tgggaatttc caccacctct tcagccagaa ctgagcccat aatggcaaat tgcaccaaca
    50041 ttgtactgtc acaatcgacg ttggccccta agtcaaggac caccgttttg cccttttgct
    50101 gatgtggtaa taccgtcacc agcgccggac gctcaatccc ctccaggggc ttgagtaata
    50161 attttgccag ccccatcagt gccccggtat taccggcact gacacaggct tgcgctcgac
    50221 cttctttcac cagctccagg gccacgcgca ttgaactccc acgactggcg cggatagctt
    50281 gcgaaggccg ggcatcactg gcgataactg actgcgcagg aataatctgc agacgcgaac
    50341 gttgttcaaa gtcagcttta gcaagtaatg gcgtgatggc gtcgggattg ccgactaaaa
    50401 gaagagtgag ttgcgaatta gaattcagtg cctgcaatgc tgcaggcact gtcacggaag
    50461 ggccaaaatc ccctcccatg acatctaacg ccagggttag acgtgtcaag gtatcgtcgc
    50521 cgcccggttt ggtctttccc cagttgcctg gggaaaatcc tcactaagct tcatcacgca
    50581 gacgcgtgat tacttagcga tgaccttgcg gccgcggtag taaccgtcgg cagtgatgtg
    50641 gtgacgcagg tgtttttcac cagaagtttt gtctacagac aggctggtga ctgcggtcag
    50701 cgcgtcatgg gaacgacgca tgccacgttt ggaacgggtt ggtttattct gttgtacggc
    50761 catggacctt actcctcaat tacggtttat ccccgatcca acggggagca ccaattactt
    50821 acgctttaag ctggctaata cggcaaatgg gtttggcttt tgcacttctt caggcagttc
    50881 accaaagacc atgtccgctt cggacacttc acagtgttca gaatcatgca ccggaactac
    50941 cggcaaggcg aggatgattt catcttcaac cattgcaagc agatcgattt caccgaattc
    51001 gttaacctca atcggttcat acgcttccgg cagtgcttca gcctgttcgt ctgaacgcac
    51061 aggactaaaa caatacgttg tgtagacctg atgagtaaac ggcttcccgc aacgctgaca
    51121 ctcgagcgtt accgtcacct tcgcatcgcc gtttaacact gcgagacgtt ggttatcgat
    51181 agcgaacgac atggagcatt ccacatcact gtccacactg actacggatt cggcgacgcg
    51241 ctcaacctga tcaggggtat agataccctg gtaatcaagg cgtttttgag ccgtacgaac
    51301 cggatcgaga gtcaggggta attttacctt ttgcataggg cgcgcatatt aactttgtaa
    51361 cgtcatagag tcaaagaaaa aggcagcctg tggttgcctt ttgccaataa ttcgcacaca
    51421 ttgcgggtta ctgctttatt ttcgttcagt gctgataacg aacatcaacc tgcaaaacga
    51481 agcgcatagt ttaaaatggc tatcaccaat acgctatatc tgatggaaaa aaatatgcct
    51541 aaacttattt tagcctccac ctcgccatgg cgtcgcgctc ttctggaaaa actgcaaatc
    51601 tcttttgaat gtgcagcacc agaggtcgac gaaaccccac gcagcgacga atcaccgcga
    51661 cagttggtgc ttcgactggc acaagaaaaa gcgcaatctc tggcgtcacg ttatccggat
    51721 catttaatta ttggttcaga ccaggtatgt gttcttgatg gcgaaatcac cggtaaaccg
    51781 ttaacggaag aaaatgcccg tctgcaatta cgcaaagcca gcggcaatat cgtcaccttc
    51841 tatactggac tggcgttgtt taattcggcg aatgggcatc tacaaacaga agtggagcct
    51901 tttgacgtcc atttccgtca cctgagcgag gcggagattg ataattacgt gcgtaaggag
    51961 catcccctgc actgcgcggg tagctttaag agtgaaggat ttggcattac gctgtttgag
    52021 cgtttagagg gacgtgaccc taacacgctg gttggtttac cgctaatcgc gctgtgtcag
    52081 atgttacgtc gggaagggaa aaacccgctg atgggatagt tgtttgcgct ggatgcggtt
    52141 acgtcgcatc cggcaatcgg tgcaggcggc ctgatgcggc gtaaacgcct tatcaggcca
    52201 accgcccatt atcaggctta tatgcttgta ttagcgcgcg ttacgcagct tttgcaaaca
    52261 acgcttcaaa ccttcatcca tcggcgcttc gatacgcatc acctcaccgg tccccggatg
    52321 ggtaaacttc aacgctgcgg cgtgcaggaa cagacgattt aatcccgtgc ctgcttcagt
    52381 gagctgtctg tcaaattcac ggtcaccgta gcgatcgtca aaggcaatcg gatgacccgc
    52441 atactgtgta tgcacacgga tctgatgagt acgtccggtt accgggctac aacgcaccag
    52501 ggtggcaaat gcatagcgtt cttccacttt aaagcgtgtt tccgacggtt tgccttcctg
    52561 actgacacgc acgatacgtt cgccgctttg cagaatattt ttcaataacg gcgcttgaac
    52621 gctcttcaca tgcgactgcc actgaccgcg caccagcgcc aggtaatctt tttgcattcc
    52681 tttttcacgt aattgctcat gcagagaacg caacgccgag cgttttttcg ctatcagcaa
    52741 cacacctgaa gtgtcccggt cgagacgatg aaccagttca aggaaccgcg cttccgggcg
    52801 caacgcccgc aaaccttcga taacgccgaa gcttaaacca ctgccgccat gtaccgccgt
    52861 accggaaggt ttattcagca ccaggatatg atcatcttca tataagatga cgtccgccag
    52921 cgccgccacc ttttgcagat gtggcgaaac cgcctcttct tcccgctcag caacgcgaac
    52981 cggtgggata cgcacctcat cacccgcttc gagtttatat tcaggcttaa tacgtttttt
    53041 gttcacccgc acttcgcctt tacgcaaaat acggtaaatc atactttttg gtacgccttt
    53101 caattgggta cgcaaaaagt tatcgatacg ttgccccgct tcgtcagcgg tgatagcaac
    53161 aatttttacg gatggagtct ctgttttcat ggtgggcgat tctaattagc caacaggatt
    53221 cgcgccactc atttttctat gcttatattt agttttgcac cttattactt cactacgtga
    53281 tcactttatt gatggttatt aaaccaatca ccagcaagaa gtgaaaaaac tgtgagtaag
    53341 cgggtgataa atggtaaaag tcatcttgct ataacaaggc ttgcagtgga ataatgaggc
    53401 cgtttccgtg tccatccttg ttaaaacaag aaattttacg gaataaccca ttttgcccga
    53461 ccgatcatcc acgcagcaat ggcgtaagac gtattgatct ttcaggcagt tagcgggctg
    53521 cgggttgcag tccttaccgg tagatggaaa tatttctgga gagtaatacc cagtctgttt
    53581 ctttgataat tgcgctgttt ttccgcatga aaaacgggca accgacactc tgcgcctctt
    53641 tgagctgacg ataaccgtga ggttggcgac gcgactagac acgaggccat cggttcacac
    53701 ccggaaaggc gttactttgc ccgcagctta gtcgtcaatg taagaataat gagtaagtta
    53761 cgatgaaaag aatgttaatc aacgcaactc agcaggaaga gttgcgcgtt gcccttgtag
    53821 atgggcagcg tctttatgac ctggatatcg aaagtccagg gcacgagcag aaaaaggcaa
    53881 acatctacaa aggtaaaatc acccgcattg aaccgagtct ggaagctgct tttgttgatt
    53941 acggcgctga acgtcacggt ttcctcccac taaaagaaat tgcccgcgaa tatttccctg
    54001 ctaactacag tgctcatggt cgtcccaaca ttaaagatgt gttgcgtgaa ggtcaggaag
    54061 tcattgttca gatcgataaa gaagagcgcg gcaacaaagg cgcggcatta accaccttta
    54121 tcagtctggc gggtagctat ctggttctga tgccaaataa cccgcgcgca ggtggcattt
    54181 ctcgccgtat cgaaggcgat gaccgtaccg aattaaaaga agcactggca agccttgaac
    54241 tgccggaagg catggggctt atcgtgcgca ccgctggcgt aggcaaatct gctgaggcgc
    54301 tgcaatggga tttaagcttc cgcctgaaac actgggaagc catcaaaaaa gccgctgaaa
    54361 gccgcccggc tccgttcctg attcatcagg agagcaacgt aatcgttcgc gcattccgcg
    54421 attacttacg tcaggacatc ggcgaaatcc ttatcgataa cccgaaagtg ctcgaactgg
    54481 cacgtcagca tatcgctgca ttaggtcgcc cggatttcag cagcaaaatc aaactgtata
    54541 ccggcgagat cccgctgttc agccactacc agattgagtc gcagatcgag tccgccttcc
    54601 agcgtgaagt ccgtctgccg tccggtggtt cgattgttat cgacagcacc gaagcgttaa
    54661 cggccatcga catcaactcc gcacgcgcga cccgcggcgg cgatatcgaa gaaaccgcgt
    54721 ttaacaccaa cctcgaagct gccgatgaga ttgctcgtca gctgcgcctg cgtgacctcg
    54781 gcggcctgat tgttatcgac ttcatcgaca tgacgcctgt acgccaccag cgtgcggtag
    54841 aaaaccgtct gcgtgaagcg gtgcgtcagg accgtgcgcg tattcaaatc agccatattt
    54901 ctcgctttgg cctgctggaa atgtcccgcc agcgcctgag cccatcactg ggtgaatcca
    54961 gccatcacgt ctgcccgcgc tgctccggta ccggtaccgt gcgtgacaac gaatcgctgt
    55021 cgctctctat tctgcgtttg atcgaagaag aagcgctgaa agagaacacc caggaagttc
    55081 acgccattgt tcctgtgcca atcgcttctt atctgctgaa tgaaaaacgt tctgcggtga
    55141 atgcgattga aacgcgtcag gacggtgttc gctgcgtgat tgtgccaaac gatcagatgg
    55201 aaaccccgca ctaccacgtg ttgcgcgtgc gcaaagggga agaaacgccg accttaagct
    55261 acatgctgcc gaagctgcat gaagaagcga tggcgctgcc gtctgaagaa gagttcgctg
    55321 aacgtaagcg tcctgaacaa cctgcactgg caacctttgc catgccggat gtgccgccag
    55381 cgccaacccc agctgaacct gccgcgactg tcgtagcccc agcaccgaaa gctgcaacgg
    55441 caacaccagc agctcctgca caacctgggc tgttaagccg cttcttcggc gcactgaaag
    55501 cgctgttcag cggtggtgaa gaagccaaac cgaccgagca accaacaccg aaagcagaag
    55561 cgaaaccgga acgtcaacag gatcgtcgca aacctcgtca gagcaaccgc cgtgaccgta
    55621 atgagcgccg cgacacccgt agtgaacgta ctgaaggcag cgataatcgc gaagaaaacc
    55681 gtcgtaatcg tcgccaggca cagcagcaga ctgccgagac gcgtgagagc cgtcagcagg
    55741 ctgaggtaac ggaaaaagca cgtaccaccg acgagcagca agcgccgcgt cgtgaacgta
    55801 gccgccgccg taacgatgat aaacgtcagg cgcaacaaga agcgaaggcg ctgaatgttg
    55861 aagagcaatc tgttcaggaa accgaacagg aagaacgtgt acgtccggtt cagccgcgtc
    55921 gtaaacagcg tcagctcaat cagaaagtgc gttacgagca aagcgtagcc gaagaagcgg
    55981 tagtcgcacc ggtggttgaa gaaactgccg ctgccgaacc aattgttcag gaagctccag
    56041 ctccacgcac agaactggtg aaagtcccgc tgccagtcgt agcgcaaact gcaccagaac
    56101 agcaagaaga gaacaatgct gataaccgtg acaacggtgg catgccgcgt cgttctcgcc
    56161 gctcgcctcg tcacctgcgc gtgagtggtc agcgtcgtcg tcgctatcgt gacgagcgtt
    56221 atccaaccca gtcgccaatg ccgttgaccg tagcgtgcgc gtctccggaa ctggcctctg
    56281 gcaaagtctg gatccgctat ccaattgtac gtccgcaaga tgtacaagtt gaagagcagc
    56341 gcgaacagga agaagtacaa gtgcagccga tggtgactgg ggtccctgtc gccgccgctg
    56401 tcgaacctgt tgttagcgca ccggttgttg aagaagtggc tgaagttgta gaagcccccg
    56461 ttcagattgc cgaaccgcaa ccggaagtgg ttgaaacgac gcatcctgaa gtgattgccg
    56521 ccgcagtaac tgaacagccg caggtgatta ccgagtctga tgttgccgta gcccaggaag
    56581 ttgcagaaca cgcagaaccg gtggttgaac cgcaggaaga gacggcagat attgaagaag
    56641 ttgccgaaac tgctgaggtt gtggttgctg aacctgaagt tgttgctcaa cctgccgcgc
    56701 cagtcgtcgc tgaagtcgca gcagaagttg aaacggtagc tgcggttgaa cctgaggtca
    56761 ccgttgagca taaccacgct acggtgccga tgacgcgcgc tccggcaccg gaatatgttc
    56821 cggatgcacc gcgtcacagt gactggcagc gccctacttt tgccttcgaa ggtaaaggtg
    56881 ccgcaggtgg tcatacggca acacatcatg cctctgccgc tcctgcgcgt ccgcaacctg
    56941 ttgagtaata attagctcaa gtaatcaagc cctggtaact gccagggctt ttttatttca
    57001 tctttgaatc aacatattaa aattcatcga atggcatcct tgctaaccaa caatgcaaaa
    57061 taggcaaaaa aatgcccgcc ccggcgatcc cagagcaggc agacaaaaca tacccagttt
    57121 catgatatgt tttaaagcga aattatttgt tgagctggaa gagcgacaat ccctgcatat
    57181 cggtaaatgc tttatacgat gcctgcaatg ccgtttgctg catgatgtaa gatgaaatag
    57241 ttgcattcca gtccacatca accagatcgc tcatctgctg cgtttgccct aaagcgcgat
    57301 cgctacctaa tgaatccagc gactccagtt cgttcagctg cgtgcctaat tccgcgcgaa
    57361 cggtcagcac attgttcagc gagtttttca gtccgcggtt ggttttatct aacgccgcag
    57421 cggcggtttc tttatccgct tcgctatccg cgaccggcgt tttcagggct gcgatggcgc
    57481 tatccagcat ggcaaaaaga ttggtttcag aagcgctacc gtctggttcc gctaccgcgt
    57541 tactggtaat actgtcgaaa attttgtcgc ccgtatgtcc tatcaccatc gaacgcgaag
    57601 catcgacctg ttgcttaata ctttctgttc cgccgacata atcaccgtta gcctcactaa
    57661 acggcgcagt ctctgtttta taaccggcaa aaatgtagcg cccattaccg tcagtggtgt
    57721 tcgccagatt cagcaactgg tcacgaagcc cctgaatatc cgtagccagc gaggcccggt
    57781 catcgtcact caaggtgcca ttgctggcgt agacaatttt ttcctgagca ttctggatag
    57841 cagtggtgac ctggctaagt acactctctt ccagtgacac tttttgagtg gcgaaagtac
    57901 gcgccagcgt gtactggctg ttttgcgcct gtgcctggga gagaacaacc gcttgtgatg
    57961 cagcaatggg atcatctgaa gggttaacga ctcgcttacc cgtcgacatc tgttcgccgt
    58021 acttcatcca ttctgcctga gaattggtga taccacgcat gttttgctgg tacatcatct
    58081 gggtactgaa acgcatttta tctcccctta gcgaatgtta atcagcgcat caaaaatcgc
    58141 gtttgccgtc tgcagaacct gcgcattcgc caggtaatac tgctgaaaac gttgcagatt
    58201 tccgtactcc tcatcgagat tgacaccgga aatcgactgc tgctgattgg aaagctgcgt
    58261 caccacatta ccttgcgtgg tgctactggt tttcaacgtc gcggttttat taccgatatc
    58321 actcactaac gaggcataag cgtcgttaaa ggatttcgca ccgcccaccg ttttactgtt
    58381 gctttgcaga tccagcaggg cctgaccgtt gcggttatcg ctatcacccg catctttttc
    58441 gctcgccatc gctattttcg cttcgtcggt gattaataca tccatgttga cgatggcgtc
    58501 acttactggt ttcagcgtga agctgtcgtt aacggcaggc gttcctgtaa acgtcaactc
    58561 cagaccatca aatgccactt taccgttggc atccggcgtc accgtaaaag tggtattgct
    58621 ggcaaggcgg gtgacctgcc actgattatt atcgaacgag attttgtaat ctgtcgccag
    58681 taccacggag gcatcagtta ccgtggcacc gatcgcaacg tcacctttgt ttttcgtgtt
    58741 ttgcagaacc gcgggcttac cgatagtaaa gaaatcttca ccagcatcgc cgttagcatc
    58801 aaatccggct ttgtgttggg tgttgaaagc ctcggcaaat gccagcgcca gttgtccaag
    58861 cgtattacgc gtctggtcca gctcctgaga acggaatgtc agaatgccgc ccagcgaccc
    58921 ggtattcagt aatttctccg ggatctcaat attgcctgcc gtcccatcaa cataagcgac
    58981 agtcgtacga gaagggtcag cgctggaagg aactgccgcc agttgccgcg ccgtacttcc
    59041 ctgaaccagt gagtaaccat tggccaccgt gatgttataa gtgccgccat cctgaacgct
    59101 gacttctaca ccaacaatct ggtttaattc gctcaccagt tgatcgcgtt gatccagcag
    59161 attgttaggt gacgcccctg cccccacgcc tgtcaggcgc gagatttgat cgttcaggct
    59221 ggcaatttgt ttagcgtaat tgttgatctg atcaacgctg gcacctatcg cgatattgac
    59281 ctgtttgtcc tggtcgcgca gatattgatc ggtggtttta aactgattca ccaacccttc
    59341 tgatttccca atcagcgcct ggcgcgctgc cgggtcttcc gcgttactca ccagcgtttg
    59401 caggctggtg aagaaatcct gcatctgtgt tgccagcgaa gaggtactgg tggagagcat
    59461 attgtcgatt ttcgacatct gctcatagcg ggcggtcagg ccgctacttt gcgtctgcgc
    59521 agcacgtaac tggttggtaa taaacgcatc atactcacgc tgcacaccag aaacgtagac
    59581 gccattgcca acccagccgc cagcgcccaa cgtgctattg gcctgcgcca taatagtggt
    59641 ttggcgggta tatccggcaa cgttatagct ggagatatta ttacttgccg tatttaacgc
    59701 cgcctgggcc gcgttcagtc cgctcatggc gttattgatc aagctggaca tggaggttcc
    59761 ttataagcct tcaattacag agtattatcg gcagcgaccc gccggacttg agttattcag
    59821 aacagattat caatgttcat gctgtaggtt ttgctcacct tgtcgcttat cgatttcatc
    59881 tgctggatca tgttggtgag tttacgggca tagtgaggat cggtagcata ccccgcgtcc
    59941 tgtagggcct gcgccccctg ctccgcactc gcggctgtcg tcacggcggc gtagcgcggg
    60001 ttacgtgtta acagcccaac gtaatccgac aatgcttcca gatacgagct gtagacccga
    60061 aatttcgctt ttactttctt cgcttcgcca ttttcatatt cagtcgtggt gatttcagta
    60121 actggacctt tccagttgcc agaggctttg acgccaaaca ggttatagct cggctcgccg
    60181 ttttcgcggc ggatttgccg ttgcccccaa ccagactcca gcgccgcctg agcgaggatc
    60241 aaatgatgtg gcacaccgct ttgctggctt gccagttggg cgggcagcga gagttgcgcg
    60301 aggaatgctt tactgtcacc cggcagcgaa tcatcgtagt tacgtggcac ggccttttgc
    60361 accagctgcg aaagcgcctg attttgataa cgcaccacag tttcgagcgg gaatttcatc
    60421 ggtgctgctg gcgtggactc ctctggcaat ggttgttctg gcgtcatctg tttaaccatc
    60481 atctctgcaa gccccagacc tttgcccgcc gtcatctgtt gggcaatctg ctggtcatac
    60541 atactggtat acaggcgagt gtgctcgctg ctgaacaggc catcttttgg taaagcgtcg
    60601 cgcatgcttt tcaacatcat ctgcacgaac atcccttcca cctgacgggc caccggacgg
    60661 atatttgccg ccggatcttc gcccgctttc gcctttagtt cgttgagtga ttgcgcatcc
    60721 caggccgcac ttgccagtag tttgctgtcg ctgatcatca gatgatttcc agttttgccc
    60781 gcagacatcc cgcactttgc attgattgca gtatggacat cagatccatc ggcgtagcgc
    60841 ccagcgcatt gagcgcgcgc accacgttat tgaggctggc gctggaacgt acgctttgca
    60901 gcgaaccgcc gctctggcgt aaatcgatct gcgtttgtgg agtaaccaca gtctgtccac
    60961 caccaaacgg tgtatctggc tggctgacat tggcctgacg attgactgtt actgagagat
    61021 ttccctgcgc taccgcgcag ctgtcgaggg tcacttcgcg attcatcacc accgaaccgg
    61081 tgcgcgagtt aatcactact ttagcgtcct gcggggtgac attaacctgc atattctgga
    61141 tatctgcaag gaaacggacc tgggaactgt tgccacttgg tacgcgcacc tgaatagtcc
    61201 gcgcatctaa cgcggtggcg ctgccatatc cacgcacgcg gttgatggtg tcagcgattt
    61261 gctgcgccat gctgaaatct tcgtcgttaa gttgcaaatt aagggtattc ccgacgccaa
    61321 actggctggg caattcacgt tcaataaccg caccattggt gatccgtcca ccgttcagtt
    61381 ggttcacctg aacactgcta ccgccagcgg aggctcctgc gccgccaacc agaatattgc
    61441 cctgcgccag cgcatacacc tgactgtcaa cgcccttaag cggcgtcatc aacaacgtac
    61501 ctccacgcaa gcttttggca tttcccatgg aagaaaccac cacatcgatg gtttgcccct
    61561 gacgtccaaa cggaggaagt gactctgtca ccattaccgc agcgacgttt tttagctgca
    61621 tattggtgcc cgtcggaacg gtaattccca gctgtgagag catgttatta agcgtttgtg
    61681 tggtaaacgg cgtctgggtt gtctggtcac cggtgccatc cagccccacc accagaccat
    61741 agccaatcag tgagttttgc cttaccccct gaacactggt gagatcgcga atacgctcag
    61801 cctgagccgc cgtcgtaacc agtagaagaa ttaatgcaga gagaaattta atcaccacag
    61861 cctcacttac attggcgaca ggttaaggaa gaaacgctgc aaccagccca tattttgcgc
    61921 ttcgttaatg tagccattgc ctacgtattc aatgcgcgca tccgccacct gagtagacgg
    61981 tacggtattg ctgccgctga tagtgcgtgg attaaccacg ccagagaagc gaataaattc
    62041 ggtaccctga ttaatggcaa tctgtttttc acccaccaca tgcaggttgc cgttgaccag
    62101 tacctggtca accgtcaccg tcaacgtgcc gctaaaggta ttgctggcat tggccccgcc
    62161 ctttccgttg aacgtgttac caccggaggc ttcgacatcg gcacgagcgt taccaaacag
    62221 cccctgcaaa tagcgcggca cagtatcaaa gccaaaatta gttttaccgt cacggctggc
    62281 attcgcagag gagcttttgc tggcgctgac gttctcctgc aacacgatgg tcagcgtatc
    62341 gccaatattg cgtggtcgac gatcttcaaa cagcggttga tagccatagt taatcggctg
    62401 agcagactgg aaaatagaac cgttggcgac gggcgtcgga ccgggaaccg gttgtgcact
    62461 ggtcgccccc tgcaccagcg gcgtggaggg tatccaggcg cagccggtta gtgaaagcac
    62521 caacaagctg gaaatggcat aagtatgcgc agcgtttttt tgcatggcta tcatcttcaa
    62581 aaatcagtaa accggtgaac ctgccaccgg ttaagcctta gagttgcgtc agtttttgca
    62641 gcatctgatc ggtggtggac accgctttac tgttgatttc gtaagcgcgt tgcacctgaa
    62701 tcatattgac cagttcttcc gccacgttga cgttagacgt ttcaacatac ccttgataca
    62761 gcagtcccgc gccgttcagg cccggcgtgc tttcgttcgg tacaccagag gattgcgttt
    62821 cggtgtagag gttttcgcca atgctctcca gcccggtgtc attcataaag gtggtgagat
    62881 tgagctgccc aacctgaacc ggagctgcct ggccttgttg ggttacgctg accacgccat
    62941 cacgaccgat agtgatactt aacgcattcg ccggaatggt gatcgctggc tgcacctgaa
    63001 aaccaccagc tgcccgttct gatccacctg gaaagagccg tcacgggtat aggctgatga
    63061 accatctggc aacatcacct ggaaaaagcc ctgcccttta atcgcgacat ctttgctgtt
    63121 gttggtctgc gacaggtttc cctggctgtg taagcgttca gtggcgaccg ggcgtacccc
    63181 cgtgccgatt tgtaatccgg agggtaaggt ggtttgttcg gaagactgtg cccccggctg
    63241 gcgaatggtt tgataaagca gatcttcaaa caccgcgcgc tgacgcttaa aaccgttagt
    63301 actgacgttt gccaggttgt tggcaatgac gtccatattg gtttgctggg cgtcaaggcc
    63361 cgttttggcg atccataatg aactgatcat aaaatgtcct gtagtggatt agtgacaaag
    63421 tgcatatgtc ttcttgccgg atgcggcgta aacgccttat ccggcctaca agtgctcgca
    63481 aaatcgataa attgcgattg cccccataga cccgataaac ttgcgcatcg ggcaacttat
    63541 acttgtcatg tatcctttca attaactcat cgacagcagt tggttggcac ggcctgcgtt
    63601 atcatcgacg ctgctgatca ccttcatctg catttcaaaa cgccgcgcgc tggcaatcat
    63661 gtcgctcatt gccgcaacgg cattgacgtt actgccttcc agaacccccg acatcacacg
    63721 caaggttgga tctgcctgca gtaccggccc tcgcgtggcc tgggtttctg cgcttaaacg
    63781 aaaaatgccg tcgtcaccgc gctgcacttc gctgcccgtg gctttcacca gtttcagacg
    63841 ccctactggc gcaactgtat ttgccggatc gcccggattg agcgccgaga ttgtgccatc
    63901 ggcagcaata gtgatttccg ctccttccgg cacagcaatt ggccccgcct cgcctatcac
    63961 ctgatgcccc tgaattgtca gttgcccggt gggatcaacc tgaatgctgc cattacgcgt
    64021 atacccttcg ctgccgtcag cggtctgcac ggccagccag ccatcctgct gcaacgcgac
    64081 gtccagcggg cgcgaggtgt aatccatttt gccgggcgtc atatctgcgc ccggcgttga
    64141 cgccgtgacc aacgtgcgcg tgggcagaga aagcccttcc actggcaccg cgcgtaaagc
    64201 attcaactgc gcgcgaaaac cgggcgttga ggcattggcc agattactgg cggttaccgc
    64261 ctgttgattc agtgtctggc tggctgctcc catcgcggta taaattgcgt gatccattga
    64321 gctatcccgt cagcgattag cgtaagttaa ccagcgtgtt gaggatctgg tcctgagttt
    64381 tgatggtctg ggcgttagac tgatagttac gctgggcaac gatcatattg accagttctt
    64441 tactgagatc gacattggac gcttccaacg caccgttggt cagggtgcca aagttgcccg
    64501 tcccggctgt ccccaacagc gccacgccag aagattgcgt cgcagaccag acgttgtcgc
    64561 cttcggatgc cagaccttcg ttgttggcaa agttcgccag tacaatctgc cccagcagtt
    64621 gtgtttgttc gttggaatag ttgccgacaa ccgtaccgtc atcattgatt tgataactca
    64681 ccagatcgcc cggtttgtag ccattctggg tggttgccac aatattgtta gcgccggtat
    64741 tttgctgcat ggagttgagg aagctcaggc taaacgtggc gggatctgcg ccgttaattg
    64801 cgccagtggt aatattctct gttggatttg aggtcagaac gccattggca ttaaacacca
    64861 gcttcattgc aggctcggct gtacctgttg gatcactgct atcctgggtg tagacctgcc
    64921 agttattatc cccggtcttc acaaagtaga cgctcatgtc atgagcatta ccctgactgt
    64981 cgaaaacagt caccgaacct tttttgttat agctatccgc attgctggcg ctgaatggcg
    65041 taacagtagg aagcggatca ctggaattca ggttgatctg catcgacgcc gtggtggtag
    65101 ttttcgctgc catcagggta ttcgggatcg aaatattggt cggattcgcc ccttgctgaa
    65161 tagtcggcgg cgtaccggtt gccgggtaac ccgtcagctg taaaccttgc atattcacca
    65221 ggttacggtt ttcatccagc ttaaattgtc cgttacggct gtagaacacc gaaccgttgc
    65281 tgtctaccag acggaaaaaa ccgttctggc tgatagcaac gtccagacct cgcccggtgt
    65341 tggtggtcgt gccatcggta aagtcctgag tgataccggc aacttttacc cccagtccca
    65401 ctttcgaacc ggcaaacata tcggcaaaag aggccgtgcc tgatttaaag ccgtaggtgg
    65461 cggagttggc gatattgttg ccaataacat cgaggttggt ggcggcagcg tttaatccgc
    65521 taaccgcttg agaaaaggcc atgactgact cctgaaatgt gaaggcttaa attatctgcc
    65581 gtacttcgtc gagggtggtg gtgccgtaag tgccgagatc cagcgtatta ccgctgttgc
    65641 cgcggatcac accctgcacc agagcaaact gcagcggctg ggcaaccagt tgtgtaccac
    65701 cgttactggc gctaatcgct acattgtaag aaccgttcgg cgcagtgctg ccatcagtca
    65761 acgtaccgtc ccaggtgaaa ctgtggactc cggcggtcag ttcaccaata tcaatggtgc
    65821 gcacaaccgc gccattttta tcggtgatgg tggccgtaac tttgtctgcc gcctgttgca
    65881 gctcaacacc aaacggcgtg gtcgtggtca cagccccttc ttcactgccg gttcccgcaa
    65941 gaacagtggt gccggggatc atcacgccgt gaccgatcag gttactggcc tgtaacgact
    66001 ggctgttatc aatctgtccg gaaatagatc cgagcgtggt attgagtttt tcaatcccgc
    66061 tgaccgtgct gatttgtgcc aattgcgacg tcagctcgtt gttttccatt ggattggtcg
    66121 ggtcctggtt tttcagctgc gccaccaaca aagtcagaaa actgctttgt aagtctgcgg
    66181 cgttgctgcc cgtgagcgaa ctactgctgg tggtactgac gccggtattt gtcggatcgg
    66241 tggtggttac cgcaatggac atagctttct cctttattga ccgagcgtaa gggttttcag
    66301 catcatgctt ttcaccgtgt tgagcacttc aacattggcc tgatagctgc gtgacgccga
    66361 catggtgtta accatctccc cgacgacatc aacgttcggc atttttacgt agccctttgc
    66421 atccgccagc ggattacccg gttcataaac cagtttgtcc ggggcctgac tttctataac
    66481 atcggcaacc tttacgccgc ctgtcgcagc acctggtgca gcgttaacct ggaataccac
    66541 ctgttttgcc cgatatggct gtccatcggg accggtcacg ctatcagcat tcgccagatt
    66601 actggccgcc acgttcaagc gctgggactg ggcagttaac gccgacccgg cgatatcaaa
    66661 aatattcagc agtgccatcc gttaatttcc gctctgtaaa acgttcatca tgcctttgat
    66721 ttgcccgctc aacgcgctaa ggctcatctg gtattgcagg ctgttatcgg caaactgggt
    66781 gcgttcgcga tccatatcga cggtattacc gtcaagcgaa ggctggtccg gaatacggta
    66841 ttgcagttct gcggtaggag gcgtcagcgc ctgcgccgga atgtgttgcg ttgaggtcat
    66901 cgtcagtgca accacactgg ttgcatcccg tccacgttgc atgacttttt taagttcact
    66961 ggcaaaatcg atatcgcgcg cctgataacc aggggtatcg gcattggcga tgtttgctgc
    67021 cagcacttcc tgacgctggg cgcgcagatt gagcgcctct tgttgaaaac gtaaggcggc
    67081 gtcgagctta tcgagcatat ctcctccgca ggtatcaaaa ttctgccatc agcttaaatg
    67141 cctttacgcg cgcgttatcg gcggaataaa cgcaaaatgg gtcgctattt atgccgttga
    67201 tgaccattgc ggacaggtac aattcacgtt gtagaaatgg ctgggggcga aaatgctggc
    67261 aataaaacgt agcgtggcga tcatctcgat actattcact ccgttaagtg cggcgagtaa
    67321 tctcacatcg caattgcaaa ccttttttag cgcccagctc gcgggggtaa gtgatgaggt
    67381 tcgtgtttct attcgtacag cgcccaatct actaccgccg tgcgagcagc cattgctttc
    67441 gatgagcaat aattcccgcc tgtggggcaa tgtgaatgtg ttggcacgct gcggtaacga
    67501 caaacgatat ttacaggtta atgtacaggc cacaggaaat tatgtggttg ccgcgatgcc
    67561 cattgtgcgg ggaggaaagc tggaagctgg caatgtcaaa ctaaaacgcg gacggcagga
    67621 taccctgcca ccgcgtacgg tgctggatat caatcaactt gttgatgccg ttagcctgcg
    67681 cgatctatca cccgatcaac ctatccagtt aacccagttt cgccaggcat ggcgggtaaa
    67741 agcgggacaa cgcgtcaacg tgatcgccag cggtgacggg tttagcgcca acgcagaagg
    67801 tcaggcgctg aacaatgcag ccgtcacaca gaatgcgcgg gtgcgcatgg tatcgggact
    67861 ggtggtcagc ggcgttgttg atgcagatgg gaatattctt ataaaccagt aagctgttaa
    67921 agattacccg tcccttgccg ataaataagc aacacatgat aaaagcgccc tcaatgagga
    67981 ataaaccatg agtattgatc gcacttcgcc tctgaagccc gtaagcaccg ttcaaccacg
    68041 cgaaaccact gacgcgtcgg taacgaacac ccgggcggca aaaacaacag cctccaccag
    68101 caccagtgtg acgttaagcg acgcgcaagc aaaactgatg caacccggca gcagtgatat
    68161 caatcttgaa cgtgtcgaag cgttaaaact ggcgattcgt aacggtgaac taaaaatgga
    68221 caccggcaaa attgccgatg cgctgatcaa cgaagcgcag caagacttgc agagtaactg
    68281 atcgtatgac acgtcttgca gagatcctcg accagatgtc cgctgtgctt aacgatctca
    68341 aaacggtaat ggatcaagaa cagcaacatc tctctatggg gcagatcaac ggcagccagt
    68401 tgcaatggat tacagaacaa aaaagctcac tgctggcgac gctggattac ctcgaacagt
    68461 tacgcaggaa agaacccaat acagcaaata gcgttgatat tagtcaacgc tggcaggaaa
    68521 ttactgtgaa aacgcagcaa ctacgccaaa tgaatcaaca taacggctgg ttactggaag
    68581 gacagattga gcgcaatcaa caggcgctgg aaatgttgaa accgcatcag gaaccgacgc
    68641 tatatggggc gaacggtcag acctcaacaa cccatcgcgg cggtaaaaag atttcgatct
    68701 gaatattatc tccggcctgc actgcaggcc ggaatgcatt gttacaccgt ccggcgggca
    68761 aattctttaa ctttgaagcc cagtaccgcc agtgcagcga agtacgcggc aatccccgcc
    68821 agcacgaccg ccattaaacg cagtaagcgc cagggcatgg tacccaatga ccactccggc
    68881 atgatatgta acatacctaa aagcacgcca gacatcacca gtaccgccac caccagacgc
    68941 aacagaaacg ccatccagcc gggttgcgga gtaaagattt tctgcttacg caactgccag
    69001 taaagcagcg aagcattcag acacgccgcc agaccaatag aaagtgacag cccggcatgt
    69061 ttcaacggac caataaacgc caggttcatc aattgcgtta aaatcagcgt aacgatggca
    69121 attttcactg gcgttttaat gtcctggcgg gaataaaagc caggagccaa cactttcact
    69181 acaatcaggc caatcaaacc caccgagtag gcaattaacg cccgctgggt catcagcgca
    69241 tcaaacgcgg taaatttacc gtactggaac agcgaaacgg ttaacggacc cgaaagaatg
    69301 cccaacgcaa ccgcactcgg cagcgccaac aggaaacaaa gacgcaaccc ccagtccatc
    69361 aaacggttgt attcatcatg attgccactg gcaaaacttt tcgacagcga cggcagcaaa
    69421 atggtgccaa gcgccacgcc cagcacaccg gagggaaact ccattaagcg gtcggcgtaa
    69481 tacatccaag acaccgaacc ggaagcaaga aacgaggcaa aaatggtgtt gatgattaag
    69541 gagatctggc tcacagagac gccaaggatc gccggtccca tctgtttcac cacgcgcatt
    69601 gctccggcat cgtggaagtt aatgcgcggc aggaccagca tgccgatctt cttcaggtgc
    69661 ggtagctgat acaccagctg caggatgccg ccgaccgtaa cagcccacgc cagcgccagc
    69721 accggtgggt taaagtacgg tgcggcaaac agcgcgaaac caatcatgct gatgttaagc
    69781 agtgttggcg caaacgccgg aatcgagaag cggttccacg tattcagaat cgctcccacc
    69841 agcgacgcca gggagatcag caagatatag ggaaaggtaa tctttagtag ctggctggtc
    69901 agggcaaatt tgtcagctgt gtcagcgaag cctggcgcgg tcaccatgat cacccacggt
    69961 gcggcgagca tgccagcgac cgtcacaacc gccagcgcaa gtgtcagcag gccagaaaca
    70021 taagagacaa agacccgcgt ggcgtcttca ccctgcttac ttttatattc cgccagaatc
    70081 ggtacaaatg cctgggaaaa tgccccttcg gcaaagatac ggcgtaacaa gttaggaagt
    70141 ttaaaagcga cgaaaaaggc gtcggttgcc atccctgcgc caaagattct ggcgacaatt
    70201 gcgtctcgtg cgaagccaag cacacgcgaa aacatggtca tcgagctgac ggcggccagc
    70261 gattttaata aattcatcgg tgttctaatc cagacccacg ggcaaaacgc ctgcaaggca
    70321 ggcgctgaaa gaggcgaata gtctacctgg attatggtga attgctaccg ccagatgtta
    70381 cagggtttat tcactcatcg catcgcgcca gatcttgtca acgatacgtt gcgccagcac
    70441 tgcctgttcg ccggcggttt gcggaactgt ctgattttgc acacattcaa tgaagtgccg
    70501 cgcacagccg acaaatccac gttgctcaag agtgctctgc caaccaagaa tcggtttatg
    70561 cactacgccc tgcccgcgct cctcacgcca ttcgcgcata tccgtaatgt cgatgagcgc
    70621 accgtcagtc acggcctgca cggtttcacg ctgacttccg gcacggcgat gcatacaggt
    70681 ggtgatctgc aaaggatcgg ccgaaaaatg gtgctcggca aacagcattt cgccagcgtc
    70741 gttagtcagt agcgtaccgc catccagaga ggctttgccg cccgacaacc acagcgcggt
    70801 atccaccaca tgcagataat catccagcaa cgtgaaataa agatcgtgtg gcccgacact
    70861 attgctacga tgtttatcca ttcttagcga agctgcggta gcgagttgcg tttttaactc
    70921 accgtagagt ggtgcgaaac gacggttaaa accgaccatc aacgtcagtt ttttccgcgc
    70981 cgccagttcc accagccgtt cagcatcgcg cagattttct gccagcggtt tatcgacaca
    71041 gacatgtaca cccgcattga gtaacgtact gaccacgtca aagtggctgg cggtgctgga
    71101 atgcacaaaa accgcatcgc aactggcggc aaggctggat aacgaatcgg cataaggaat
    71161 gcgccagctt tcacaaattg gcagggcttt cgcgcgcgta ggcgaccagg ctccttgtaa
    71221 catccagtca gacgctgccg ccagcaccgg taaccacgct ttttgcgcaa tgccgcctaa
    71281 tcccacaacg ccgatacgta attttttcac tttaatctcc cagatgggcc agcaacgaat
    71341 caagacgctg tttcagttct gccacttcga tttccagggc ttcgacgcgg gcctgtaaat
    71401 caccatcaac cgcgttcgac atatccgtta ccgccggctg atcctctacc tcaccactga
    71461 aaagatgcat gtagcggctt tcgcgtttac ccggttcgcg ggccagacgc accacaaaag
    71521 gaccatcttc gcgatttgcc agttgttcca gcgtcgactc cacttccgcc atatcgctga
    71581 attcatacat tcgcgcggcg cggctgcgca gttcacccgg cgtctgggca ccacgcaata
    71641 acaacgtggt gattaacgcc acttccgctg cgctcagttt cagatcgcca aattctgaat
    71701 tacaaaaacg ttgttcatat ttggtgaccc gattaccaaa gccactcacc gtgcgcagat
    71761 aatggcgttt gaccagatta tccagttgct cctgcacttc agattcactc agattcatga
    71821 ccggttcacg gttcgttttc tgattacagg ccgtgactac accattgact gagagcgggt
    71881 attgctccgg cgtggtcacc tgcttttcca gcaagcaacc aatcacgcga gcttcaaggg
    71941 cagtaagttg atatttcatg agattctcct tagcggccgg gcgtccagtc tggggtagtt
    72001 aatgccgtca gtacgtgatc gcgccattgt ccatcaatca acagatagtc tttcgcatag
    72061 ccttcttttt caaaacccag tcgcgccagt aaatcaccgc tgcgtttatt gtgcggcata
    72121 taattagcca taatgcgatg aatatgttgg gtgcgctgca tataacgaat ggctgcggtc
    72181 agggcttcaa acatgagtcc tttgccctgc catttttgcc caatcgaata accgagatag
    72241 caggcatgaa aagagccacg aacaacattg gaaaaattgg caacgccaat aatctctttt
    72301 tcgtccgggt cgaataagcc aaagtagaaa gctgaacctt gtttatgaaa ttcgttaatc
    72361 atccccagcc tggcctgcca gcctgatgga taacagtggc tttcgtcgcg cactggctcc
    72421 cagggcttga ggaaatggcg attctctgcg taataatccg caagacgcca ggcatcacga
    72481 tcatgcacca gacgcacgac cagtcggtct gtggttaagc gcacttttgg cacgttactg
    72541 cgatagccaa acattctata cctactcctt cccgtaacgt ctttaatacg ctgtaagtat
    72601 tactatacct gcgcaaccag accctgtgaa aagtccaaaa tgccattttc tgcattattc
    72661 acatatttga taaaaactct ctatcaaagc gacgttttga taaaaaaata ttgtccccga
    72721 agtgatggga aaagtcttag cggcaatgca gaatagaaag tgctcatctt ttatttcccg
    72781 ggaggggaaa tgtcccgcgt atcgcaggcg aggaacctgg gtaaatattt cctgctcatc
    72841 gataatatgc tggtcgtgct ggggttcttt gttgtcttcc cgctgatctc tatccgcttc
    72901 gttgatcaaa tgggctgggc cgccgtcatg gtcggtattg ctctcggtct acgccagttt
    72961 attcagcaag gtctgggtat tttcggcggc gcaattgccg accgctttgg tgccaaaccg
    73021 atgattgtta ccggtatgct gatgcgcgcc gccggattcg ccacaatggg tatcgcccac
    73081 gaaccgtggc tattgtggtt ttcatgcctg ctctcgggac tcggcggcac gttgtttgat
    73141 ccgccgcgtt cggcgctggt ggtgaaacta atccgtccac aacaacgttg tcgttttttc
    73201 tcgctgttga tgatgcagga cagtgccggt gcggtcattg gcgcattgtt ggggagctgg
    73261 ctgttgcaat acgactttcg cctggtctgc gccacagggg ccgttttgtt tgtgctgtgt
    73321 gcggcgttca atgcgtggtt gttaccggca tggaaactct ccaccgtacg cacgcccgtt
    73381 cgcgaaggca tgacccgcgt gatgcgtgac aagcgttttg tcacctatgt cctgacgctg
    73441 gcgggttact acatgctggc tgtacaagtg atgctgatgc tgccaattat ggtcaacgac
    73501 gtggctggcg cgccctctgc cgttaaatgg atgtatgcca ttgaagcgtg tctgtcgtta
    73561 acgttgctct accctatcgc ccgctggagt gaaaagcatt ttcgtctgga acaccggttg
    73621 atggctgggc tgttgataat gtcattaagc atgatgccgg tgggcatggt cagcggtctg
    73681 caacaacttt tcaccctgat ttgtctgttt tatatcgggt cgatcattgc cgagcctgcg
    73741 cgtgaaacct taagtgcttt gctggcggac gcaagagctc gcggcagcta tatggggttt
    73801 agccgtctgg gtctggcgat tggcggcgct attggttata tcggtggtgg ctggctgttt
    73861 gaccagggca aatcggcgca ccagccagag cttccgtgga tgatgctggg aattattggc
    73921 atcttcactt tccttgcgct gggttggcag tttagtcaga aacgcgcaac gcgtcgtttg
    73981 ctggaacgcg acgcctgatt tgatactccc ccctgactcc tccatactga aagtgaactg
    74041 acagccaaat ggaggagtca cgtgaagcta tacatttacg atcactgccc ttactgcctc
    74101 aaagcccgca tgattttcgg cctgaaaaat atccccgtcg aattacatgt tctgctcaac
    74161 gacgacgcag aaacacccac ccggatggtc ggtcaaaaac aggttcccat tctgcaaaaa
    74221 gatgacagcc gctatatgcc agaaagcatg gatatcgttc actatgtcga taaactcgac
    74281 ggcaaaccgt tactgaccgg caaacgttcc cctgccattg aagagtggct gcgcaaggtc
    74341 aatggctacg ccaacaaact gctgttgccg cgttttgcca aatcggcatt tgatgagttt
    74401 tctactcccg ccgcgcgcaa atatttcgtc gacaagaaag aggccagcgc gggtaatttt
    74461 gccgacctgc tggcccactc tgacggtctg attaagaata tcagcgatga tttgcgtgcg
    74521 ctggacaaac tgatcgtcaa accgaacgcc gtgaatggcg aactttcgga agatgatatt
    74581 cagctatttc cgctactgcg taatctgacg ctggtagccg gaatcaactg gccaagccgc
    74641 gttgctgatt accgcgataa tatggcgaaa cagacacaaa tcaatttgtt atcatcaatg
    74701 gcgatttaat cctgacccgc cgcggcagcc tgtattcgcg ggttgccgcc gttttcagtt
    74761 ttttctgctg gcgagtttat cgctggtgac acatgctgcg ttgtgtcagg atattgacgc
    74821 agatttcatg tcgaggacta ccatgaacaa gtttttattt gccgctgcat tgatcgtcag
    74881 tggcctgctc gttggctgta atcaactcac ccaatacacc atcaccgaac aagaaattaa
    74941 ccagtcgctt gcgaaacata ataatttctc aaaagatatc ggtttacccg gcgtggctga
    75001 cgcccatatt gttctgacaa acttgatcag ccaaattggt cgcgaagagc cgaataaggt
    75061 taccctaacc ggagacgcca atctggacat gaactccctg ttcggtagtc agaaagcgac
    75121 catgaaactg aagctgaaag cgctgcccgt gtttgataaa gaaaaaggtg cgatcttcct
    75181 gaaagagatg gaagtggtcg atgcgacggt acaaccggaa aaaatgcaaa cggtgatgca
    75241 aacgttgctt ccctatttga accaggcatt acgcaattac tttaaccagc aacctgctta
    75301 cgtcctgcgc gaagatggca gtcagggcga agcaatggcg aaaaaactgg cgaaaggcat
    75361 tgaagtgaag ccaggcgaaa ttgtcattcc gtttacggat taatgacgag ggcgcattcg
    75421 cgccctttat ttttcgtgca aaggaaaacg tttccgctta tcctttgtgt ccggcaaaaa
    75481 cattccttca gccggagcat agagattaat gactgcacca tcccaggtat taaagatccg
    75541 ccgcccagac gactggcacc ttcacctccg cgatggcgac atgttaaaaa ctgtcgtgcc
    75601 gtataccagc gaaatttatg gacgggctat cgtaatgccc aatctggctc cgcccgtgac
    75661 cacagttgag gctgccgtgg cgtatcgcca acgtattctt gacgccgtac ctgccgggca
    75721 cgatttcacc ccattgatga cctgttattt aacagattcg ctggatccta atgagctgga
    75781 gcgcggattt aatgaaggcg tgttcaccgc tgcaaaactt tacccggcaa acgcaaccac
    75841 taactccagc cacggcgtga cgtcaattga cgcaatcatg ccggtacttg agcgcatgga
    75901 aaaaatcggt atgccgctac tggtgcatgg tgaagtgaca catgcagata tcgacatttt
    75961 tgatcgtgaa gcgcgcttta tagaaagcgt gatggaacct ctgcgccagc gcctgactgc
    76021 gctgaaagtc gtttttgagc acatcaccac caaagatgct gccgactatg tccgtgacgg
    76081 aaatgaacgg ctggctgcca ccatcactcc gcagcatctg atgtttaacc gcaaccatat
    76141 gctggttggt ggcgtgcgtc cgcacctgta ttgtttaccc atcctcaaac acaatattca
    76201 ccaacaggca ttgcgtgaac tggtcgccag cggttttaat cgagtattcc tcggtacgga
    76261 ttctgcgcca catgcacgtc atcgcaaaga gagcagttgc ggctgcgcgg gctgcttcaa
    76321 cgccccaacc gcgctgggca gttacgctac cgtctttgaa gagatgaatg ctctgcagca
    76381 ctttgaagca ttctgttctg taaacggccc gcagttctat gggttgccgg tcaacgacac
    76441 attcatcgaa ctggtacgtg aagagcaaca ggttgctgaa agcatcgcac tgactgatga
    76501 caccctggtg ccattcctcg ccggggaaac ggtacgctgg tccgttaaac aataaaaatc
    76561 gtagcttcct gttgtcatta ggttatttaa cctgtataaa taaccagtat attcaacagg
    76621 gggctattat gcgaattgaa gtcaccatag cgaaaacttc tccattgcca gctggggcta
    76681 ttgacgccct ggctggcgaa ctttcccgcc gtattcagta tgcgtttcct gataatgaag
    76741 gccacgtatc ggtacgttat gccgcagcga ataatttatc ggttattggc gcaacaaaag
    76801 aagataaaca gcgcattagc gaaattctcc aggaaacgtg ggaaagcgcc gatgactggt
    76861 ttgtcagcga ataatatgca gtgatttttg tttttgccgg gtcgccccgg cttttttatt
    76921 ttacttacga attttgagta caaaatttct acacttcctc aaaataattg cacaatatgc
    76981 taaaaaaata tgaatgacat gctgttttat tgttcatata aatcttaaaa gcagaaattt
    77041 atctgttccc ccctttaaaa attcggtgaa tacccttact tattggtata ctgaagttgc
    77101 ttcagataaa aacgcattga acctcgaata acgttgtcta gtaacacgaa ttagggggcc
    77161 atgatggaaa aaaataatga agtcattcag actcatccgc tcgtagggtg ggacatcagc
    77221 accgttgata gctatgatgc gctgatgttg cgtttgcact accagacccc aaataagtcc
    77281 gagcaggaag ggactgaagt tggtcaaacg ctctggttaa ccactgatgt tgccagacaa
    77341 tttatttcga tattagaagc aggaatcgcc aaaattgaat ccggtgattt ccaggtaaac
    77401 gagtatcggc gtcattaaca tgacatgacc atccacccaa aaggcacctc accggtgcct
    77461 ttaccatttc tgggttgtat atctgcattc agttaattac tctgcttaaa ccatgagagc
    77521 agagagaaaa aaatgaaata cgatctcatc attattggca gcggttccgt aggcgctgcc
    77581 gccgggtatt atgcaacccg cgccggttta aacgtgctaa tgaccgacgc ccatatgcca
    77641 ccgcatcaac acggcagcca ccacggcgat acgcgattaa ttcgccatgc ttatggtgaa
    77701 ggcgaaaagt atgtcccgct ggtcctccgc gcgcaaacgc tgtgggatga actttcccgc
    77761 cacaacgaag atgatcccat ttttgtacgc tctggtgtca ttaacctcgg cccggctgac
    77821 tccgcatttc tcgccaacgt cgcccacagc gccgaacagt ggcaactcaa cgttgaaaag
    77881 ctcgatgcgc aagggattat ggcccgctgg ccagaaatac gcgtcccgga caactacatc
    77941 ggcttatttg agactgattc cggttttttg cgcagcgaac tggcgattaa aacctggatc
    78001 caactggcga aggaagcggg ctgtgcgcaa ctgttcaact gcccggtcac cgcaattcgt
    78061 catgacgatg atggcgtaac tattgaaacg gttgacggtg agtatcaggc gaaaaaagcg
    78121 attgtctgcg cgggaacatg ggtaaaagac ctgctcccgg agctgcctgt ccagcccgta
    78181 cgtaaagtat ttgcctggta tcaggccgat ggccgctata gcgtgaagaa taaattcccg
    78241 gcgtttaccg gtgaactgcc caatggcgat caatattatg gttttccggc agaaaacgac
    78301 gcgttgaaga ttggcaaaca taacggaggc caggttatcc attcagcgga tgaacgtgtt
    78361 ccgtttgcgg aagtggtcag cgatggttcg gaagccttcc cgttcttgcg caatgtattg
    78421 ccgggtatcg gttgctgcct gtacggcgct gcctgcacct atgataattc gcctgacgaa
    78481 gattttatta tcgataccct acccggccac gataatacac tgctcattac cggcctgagt
    78541 gggcacggtt ttaaatttgc gtcagtttta ggggaaatag ctgacgattt tgcgcaagac
    78601 aaaaaaagcg attttgattt gacgccattc aggctttccc gcttccaata atcatcatta
    78661 cggcctccag aatgaggccg tttttacagg agtatttaat gcgtcggctg ttgcactatc
    78721 tca
//
LOCUS       DAKISA010000005        73914 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.005, whole genome shotgun sequence.
ACCESSION   DAKISA010000005 DAKISA010000000
VERSION     DAKISA010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 73914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73914
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.005"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..942
                     /locus_tag="OVW45_000324"
     CDS             <1..942
                     /locus_tag="OVW45_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCV5524865.1"
                     /translation="PMLGEKRLVSSVTKEDLLFVRRDLLTGYQKLSNGKISSIKGRSV
                     VTVNYYMTTIAGMFQFATDNGYTSGNPFNGLTPLKKSKIEPDPLTRDEFIRFIEACRH
                     QQTKNLWIIAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGR
                     TIHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRSTVHKCTFVFSPQVIKQRQFSG
                     PHYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQM
                     VFNVYGAWMKDNNHEQIELLNKRLSESVPCMPHKKVG"
     gene            978..1664
                     /gene="mlrA"
                     /locus_tag="OVW45_000325"
     CDS             978..1664
                     /gene="mlrA"
                     /locus_tag="OVW45_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HCV5524866.1"
                     /translation="MPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIKRWIDN
                     GVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYPAQTLT
                     THLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGWNIQDT
                     TRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQLTSWQE
                     QGHDIFPLGI"
     gene            1724..1831
                     /locus_tag="OVW45_000326"
     CDS             1724..1831
                     /locus_tag="OVW45_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCV5524867.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(1812..2543)
                     /gene="yehW"
                     /locus_tag="OVW45_000327"
     CDS             complement(1812..2543)
                     /gene="yehW"
                     /locus_tag="OVW45_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCV5524868.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(2548..3474)
                     /gene="yehX"
                     /locus_tag="OVW45_000328"
     CDS             complement(2548..3474)
                     /gene="yehX"
                     /locus_tag="OVW45_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCV5524869.1"
                     /translation="MIEFGHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(3467..4624)
                     /gene="yehY"
                     /locus_tag="OVW45_000329"
     CDS             complement(3467..4624)
                     /gene="yehY"
                     /locus_tag="OVW45_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCV5524870.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(4631..5548)
                     /gene="osmF"
                     /locus_tag="OVW45_000330"
     CDS             complement(4631..5548)
                     /gene="osmF"
                     /locus_tag="OVW45_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCV5524871.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPKGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(5800..8097)
                     /gene="bglX"
                     /locus_tag="OVW45_000331"
     CDS             complement(5800..8097)
                     /gene="bglX"
                     /locus_tag="OVW45_000331"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCV5524872.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            8293..10008
                     /gene="dld"
                     /locus_tag="OVW45_000332"
     CDS             8293..10008
                     /gene="dld"
                     /locus_tag="OVW45_000332"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCV5524873.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYIHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(10047..10979)
                     /gene="pbpG"
                     /locus_tag="OVW45_000333"
     CDS             complement(10047..10979)
                     /gene="pbpG"
                     /locus_tag="OVW45_000333"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCV5524874.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNSTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(11153..11740)
                     /locus_tag="OVW45_000334"
     CDS             complement(11153..11740)
                     /locus_tag="OVW45_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCV5524875.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            11910..12488
                     /locus_tag="OVW45_000335"
     CDS             11910..12488
                     /locus_tag="OVW45_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCV5524876.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(12618..13379)
                     /locus_tag="OVW45_000336"
     CDS             complement(12618..13379)
                     /locus_tag="OVW45_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCV5524877.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLELGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(13432..14868)
                     /gene="mdtQ"
                     /locus_tag="OVW45_000337"
     CDS             complement(13432..14868)
                     /gene="mdtQ"
                     /locus_tag="OVW45_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HCV5524878.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            15092..15175
                     /gene="yohP"
                     /locus_tag="OVW45_000338"
     CDS             15092..15175
                     /gene="yohP"
                     /locus_tag="OVW45_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCV5524879.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(15548..16495)
                     /gene="dusC"
                     /locus_tag="OVW45_000339"
     CDS             complement(15548..16495)
                     /gene="dusC"
                     /locus_tag="OVW45_000339"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCV5524880.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            16734..17132
                     /locus_tag="OVW45_000340"
     CDS             16734..17132
                     /locus_tag="OVW45_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCV5524881.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            17129..17824
                     /locus_tag="OVW45_000341"
     CDS             17129..17824
                     /locus_tag="OVW45_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCV5524882.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            17954..18838
                     /gene="cdd"
                     /locus_tag="OVW45_000342"
     CDS             17954..18838
                     /gene="cdd"
                     /locus_tag="OVW45_000342"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCV5524883.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            18988..19707
                     /gene="sanA"
                     /locus_tag="OVW45_000343"
     CDS             18988..19707
                     /gene="sanA"
                     /locus_tag="OVW45_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCV5524884.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFRREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            19710..19949
                     /locus_tag="OVW45_000344"
     CDS             19710..19949
                     /locus_tag="OVW45_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCV5524885.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            20143..21381
                     /gene="preT"
                     /locus_tag="OVW45_000345"
                     /pseudo
     CDS             20143..21381
                     /gene="preT"
                     /locus_tag="OVW45_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
     gene            21375..22610
                     /gene="preA"
                     /locus_tag="OVW45_000346"
     CDS             21375..22610
                     /gene="preA"
                     /locus_tag="OVW45_000346"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HCV5524886.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(22737..23747)
                     /gene="mglC"
                     /locus_tag="OVW45_000347"
     CDS             complement(22737..23747)
                     /gene="mglC"
                     /locus_tag="OVW45_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCV5524887.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(23763..25283)
                     /gene="mglA"
                     /locus_tag="OVW45_000348"
     CDS             complement(23763..25283)
                     /gene="mglA"
                     /locus_tag="OVW45_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCV5524888.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(25344..26342)
                     /gene="mglB"
                     /locus_tag="OVW45_000349"
     CDS             complement(25344..26342)
                     /gene="mglB"
                     /locus_tag="OVW45_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCV5524889.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(26622..27662)
                     /gene="galS"
                     /locus_tag="OVW45_000350"
                     /pseudo
     CDS             complement(26622..27662)
                     /gene="galS"
                     /locus_tag="OVW45_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
     gene            complement(27804..28961)
                     /locus_tag="OVW45_000351"
     CDS             complement(27804..28961)
                     /locus_tag="OVW45_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCV5524890.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRT
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            complement(28978..29646)
                     /gene="folE"
                     /locus_tag="OVW45_000352"
     CDS             complement(28978..29646)
                     /gene="folE"
                     /locus_tag="OVW45_000352"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCV5524891.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            29904..30740
                     /gene="yeiG"
                     /locus_tag="OVW45_000353"
     CDS             29904..30740
                     /gene="yeiG"
                     /locus_tag="OVW45_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCV5524892.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(30772..32763)
                     /gene="cirA"
                     /locus_tag="OVW45_000354"
     CDS             complement(30772..32763)
                     /gene="cirA"
                     /locus_tag="OVW45_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HCV5524893.1"
                     /translation="MFRLNPFVRVGLCLSAISCVWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(33055..34524)
                     /gene="lysP"
                     /locus_tag="OVW45_000355"
     CDS             complement(33055..34524)
                     /gene="lysP"
                     /locus_tag="OVW45_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCV5524894.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(34729..35610)
                     /locus_tag="OVW45_000356"
     CDS             complement(34729..35610)
                     /locus_tag="OVW45_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCV5524895.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            35709..36758
                     /locus_tag="OVW45_000357"
     CDS             35709..36758
                     /locus_tag="OVW45_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCV5524896.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATESVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            36832..37689
                     /gene="nfo"
                     /locus_tag="OVW45_000358"
     CDS             36832..37689
                     /gene="nfo"
                     /locus_tag="OVW45_000358"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCV5524897.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            37693..38781
                     /locus_tag="OVW45_000359"
     CDS             37693..38781
                     /locus_tag="OVW45_000359"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCV5524898.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWAFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(38837..40087)
                     /locus_tag="OVW45_000360"
     CDS             complement(38837..40087)
                     /locus_tag="OVW45_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCV5524899.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(40187..41128)
                     /gene="rihB"
                     /locus_tag="OVW45_000361"
     CDS             complement(40187..41128)
                     /gene="rihB"
                     /locus_tag="OVW45_000361"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCV5524900.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGEMFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELSVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            41258..41956
                     /gene="yeiL"
                     /locus_tag="OVW45_000362"
     CDS             41258..41956
                     /gene="yeiL"
                     /locus_tag="OVW45_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HCV5524901.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(42023..43273)
                     /locus_tag="OVW45_000363"
     CDS             complement(42023..43273)
                     /locus_tag="OVW45_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCV5524902.1"
                     /translation="MDIMRSVAGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVSVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(43367..44305)
                     /gene="psuG"
                     /locus_tag="OVW45_000364"
     CDS             complement(43367..44305)
                     /gene="psuG"
                     /locus_tag="OVW45_000364"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCV5524903.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(44293..45234)
                     /locus_tag="OVW45_000365"
     CDS             complement(44293..45234)
                     /locus_tag="OVW45_000365"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCV5524904.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEALVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVRSLVENTECLN"
     gene            complement(45692..47383)
                     /gene="fruA"
                     /locus_tag="OVW45_000366"
     CDS             complement(45692..47383)
                     /gene="fruA"
                     /locus_tag="OVW45_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCV5524905.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(47400..48338)
                     /gene="fruK"
                     /locus_tag="OVW45_000367"
     CDS             complement(47400..48338)
                     /gene="fruK"
                     /locus_tag="OVW45_000367"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCV5524906.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTLADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(48338..49468)
                     /gene="fruB"
                     /locus_tag="OVW45_000368"
     CDS             complement(48338..49468)
                     /gene="fruB"
                     /locus_tag="OVW45_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCV5524907.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEVGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            49836..51016
                     /gene="setB"
                     /locus_tag="OVW45_000369"
                     /pseudo
     CDS             49836..51016
                     /gene="setB"
                     /locus_tag="OVW45_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
     gene            complement(51013..51267)
                     /locus_tag="OVW45_000370"
     CDS             complement(51013..51267)
                     /locus_tag="OVW45_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCV5524908.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            51422..51994
                     /gene="yeiP"
                     /locus_tag="OVW45_000371"
     CDS             51422..51994
                     /gene="yeiP"
                     /locus_tag="OVW45_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCV5524909.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            52217..53683
                     /locus_tag="OVW45_000372"
     CDS             52217..53683
                     /locus_tag="OVW45_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCV5524910.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKCSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFVHISDCMQDRVFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            53801..54787
                     /gene="yeiR"
                     /locus_tag="OVW45_000373"
     CDS             53801..54787
                     /gene="yeiR"
                     /locus_tag="OVW45_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCV5524911.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYELWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            54826..55539
                     /gene="lpxT"
                     /locus_tag="OVW45_000374"
     CDS             54826..55539
                     /gene="lpxT"
                     /locus_tag="OVW45_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCV5524912.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            55951..56517
                     /gene="mepS"
                     /locus_tag="OVW45_000375"
     CDS             55951..56517
                     /gene="mepS"
                     /locus_tag="OVW45_000375"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCV5524913.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            56698..58254
                     /locus_tag="OVW45_000376"
     CDS             56698..58254
                     /locus_tag="OVW45_000376"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCV5524914.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            58336..60150
                     /locus_tag="OVW45_000377"
     CDS             58336..60150
                     /locus_tag="OVW45_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCV5524915.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPSKEDMLSLFSLPIFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNETGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            60151..61245
                     /locus_tag="OVW45_000378"
     CDS             60151..61245
                     /locus_tag="OVW45_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCV5524916.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            61245..62270
                     /locus_tag="OVW45_000379"
     CDS             61245..62270
                     /locus_tag="OVW45_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCV5524917.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            62272..63861
                     /gene="yejF"
                     /locus_tag="OVW45_000380"
     CDS             62272..63861
                     /gene="yejF"
                     /locus_tag="OVW45_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCV5524918.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(63865..64209)
                     /locus_tag="OVW45_000381"
     CDS             complement(63865..64209)
                     /locus_tag="OVW45_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5524919.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(64542..65662)
                     /locus_tag="OVW45_000382"
                     /pseudo
     CDS             complement(64542..65662)
                     /locus_tag="OVW45_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
     gene            complement(65690..66385)
                     /gene="rsuA"
                     /locus_tag="OVW45_000383"
     CDS             complement(65690..66385)
                     /gene="rsuA"
                     /locus_tag="OVW45_000383"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCV5524920.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLVSPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            66535..68295
                     /locus_tag="OVW45_000384"
     CDS             66535..68295
                     /locus_tag="OVW45_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCV5524921.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NIDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRSDEKALFRDCIYELPLRYMIKHSYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIENFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRPPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            68420..68704
                     /gene="rplY"
                     /locus_tag="OVW45_000385"
     CDS             68420..68704
                     /gene="rplY"
                     /locus_tag="OVW45_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCV5524922.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(68843..69850)
                     /gene="yejK"
                     /locus_tag="OVW45_000386"
     CDS             complement(68843..69850)
                     /gene="yejK"
                     /locus_tag="OVW45_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCV5524923.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNTKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            70032..70259
                     /locus_tag="OVW45_000387"
     CDS             70032..70259
                     /locus_tag="OVW45_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCV5524924.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            70279..72039
                     /gene="pbgA"
                     /locus_tag="OVW45_000388"
     CDS             70279..72039
                     /gene="pbgA"
                     /locus_tag="OVW45_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCV5524925.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGHYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            72114..72190
                     /locus_tag="OVW45_000389"
     tRNA            72114..72190
                     /locus_tag="OVW45_000389"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:72148..72150,aa:Pro,seq:ggg)
     gene            complement(72293..73914)
                     /gene="yejO"
                     /locus_tag="OVW45_000390"
                     /pseudo
     CDS             complement(72293..73914)
                     /gene="yejO"
                     /locus_tag="OVW45_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
BASE COUNT        18140 a        19470 c        18192 g        18112 t
ORIGIN      
        1 ccaatgttag gtgaaaaaag actggtttca tctgtaacaa aagaggattt gcttttcgta
       61 aggagagatc tgttgaccgg ttatcaaaag ctttctaacg gaaaaatttc ttccataaaa
      121 ggacgctcag tggtcacagt aaactactat atgacaacca tagctggaat gtttcaattt
      181 gcaacagata atggttatac atcaggaaat ccatttaacg gtctgacacc attaaaaaag
      241 tccaagatag aaccagatcc cctcacccgt gacgaattta ttcgttttat tgaggcttgc
      301 cgtcatcaac aaacaaaaaa cctgtggatt atcgctgtat acacgggtat tcgtcacggg
      361 gagctggtat cactggcctg ggaagatata gaccttaaag cgaggactat aaccatccgc
      421 cggaattata caaaactagg cgaattcact ccaccaaaaa ctgatgctgg taccggaaga
      481 acgattcatc tagttcaacc agctattgat gctcttaaaa gccaagcgga aatgaccatg
      541 cttggaaaac agcattctgt agaggtaaag cagagggaat atgggagaag tactgtgcat
      601 aaatgcactt ttgtttttag tcctcaggta ataaaacagc ggcagttttc cggaccgcac
      661 tataaggttg actccatcag ggagtcatgg acaagtatct taaaacgcgc aggtctgaga
      721 cacagaaaat cgtaccaatc caggcatact tatgcatgct ggtcacttgc cgctggagct
      781 aatcctagtt ttatcgcaag ccagatgggc cacacaaacg cacaaatggt attcaatgtt
      841 tacggagcat ggatgaaaga caacaatcac gaacagatag aactccttaa caaaagacta
      901 tctgaaagtg tcccatgtat gccccataag aaagtggggt aaaataaaaa cttgtaaaat
      961 cagttagttt acccttaatc cctgtcacgt tacgcgcgtg gcagaggcgt tacgggttgc
     1021 tgaaaccgca acggacagac ggcggtcatc gactgttcaa cgatgccgat attgaccgga
     1081 tccgcgagat caaacgctgg atcgacaacg gcgtgcaggt cagcaaagtt aaaatgctgc
     1141 tcagtaatga aaatgttgat gtgcagaacg gctggcgcga tcagcaagaa acattactga
     1201 cctacctgca aagcggcaat ctgcatagcc tgcgaacgtg gatcaaagag cgcggtcagg
     1261 attaccccgc ccagacactc accacacatc tgtttattcc tctgcgccga cggcttcagt
     1321 gccaacaacc gactctccag gcgctgctgg cgatcctcga cggcgtactg atcaactaca
     1381 tcgccatttg tctggcttcg gcacgtaaaa aacagggtaa agatgcgctg gtggttggct
     1441 ggaatattca ggataccacc cgtctgtggc tggagggctg gattgccagt caacaaggat
     1501 ggcgcattga tgtcctcgcc cactcgctca atcaactacg ccctgaacta ttcgaaggcc
     1561 gtacattgct ggtgtggtgc ggtgaaaatc gaacctccgc ccaacagcag caactcacca
     1621 gttggcaaga acaaggccat gatattttcc cactcggcat ttaatgattc gttaacaaat
     1681 gcgctttact gtacaatcct ttcgttaaca taaggagtgc aatatgcgca tagctaaaat
     1741 tggggtcatc gccctgttcc tgtttatggc gttaggcgga attggtggcg tcatgctcgc
     1801 aggttatacc tttattttgc gtgctggcta agcgcctgca ccagcctttc aaacaggcgg
     1861 tctgcgatga tcgccgccag tgccaccagt aacgccccct ggatcacata cgcggtatta
     1921 aatccgctaa gcccgatgat gatgggcgta cccagcgtgc tggcccctac cgttgaggcg
     1981 atcgtcgccg taccaatgtt gataatcacc gaagttcgca cgcccgccag aatcaccgga
     2041 gccgccagcg gtagctcgac cttacgcagt cgctgaccac gactcattcc catacctttc
     2101 gcaacttctg tcacgctggc atcaatcgct cccagcccgg caagtgtcgc ctgcaggacg
     2161 ggcagcacac cgtaaaggat caaggcgata atcgctggtt gcagaccaaa gccgatcacc
     2221 ggaacggcaa tcgccagcac tgcgacaggc ggaaaagtct gtccaacggc ggcaatagtt
     2281 tccaccagtg ggcgaaattc cgcgccccac gggcgagtga cagcaattcc ggcaccagtg
     2341 ccaatgatca ccgcaaacaa actcgaaatt cccaccagcc agaaatgagc cagtgccaga
     2401 gctgcaaaac tttcttgctg ataaacgggt cgtggcagtt gtgggaacaa ggcagcaaac
     2461 agcggctggc tgtaaggcag ccagaaaatc agtgccacaa acaaagcaat gagccagaac
     2521 agcggatcgc gcaacatctt catacgctta cgcctcctcc agcagatcct gaaaatgcag
     2581 cgtgccgcaa ggctggccct gcgtgttcac caccggcagc acctcgcatc cccgcgcgac
     2641 aaacagggag agcgcatcgc gtagcgtcat ctcttctgcc agtgcctcac cttctgcccg
     2701 ttcttcgcga cgcacgtaat ccgccacact acgtaacgaa agcaggcgca cacccagttc
     2761 gctgcgccca aaaaactggc ggacaaaatc gtttgccgga cgagtcagca tcgtcagcgg
     2821 attgccctgc tgcaccactt caccgtgatc catcaatacc agatgttctg ccagccgtag
     2881 cgcctcatca atatcatgag tgaccagcac aatggtacgc cccagcaaac ggtgaatgcg
     2941 cgtcatctct tgttgcaacg cgccgcgcgt taccgggtcc agtgcgccaa aaggttcatc
     3001 catcagtaag acttgcggat cggcagccag tgcgcgcgcc actcccacac gttgctgctg
     3061 accaccggaa agctgatgcg gataacgctc acgtaaattt gactccagcc ccagtagcgc
     3121 cattaattcg tcgatacgat cgtcaatcct cgcccgcgac catttttgta attgcggcac
     3181 ggtagcgatg ttttgcgcca cgctccagtg ggggaacagg ccaatagatt gaatggcata
     3241 gcccatccgg cggcgcaact ccagtactgg cagcgagcga atttcttctc cggcaaagcg
     3301 gatcactccg ctgtcatgct ccaccaggcg gttaatcatt ttcagggtgg tggatttgcc
     3361 ggagccagat gtgccaatca gcaccgaaaa actcccttcc tgaaaattga gattgagatc
     3421 gttaacggct ttttgtgcgc cgaacagttt gctgacatgg ccaaattcaa tcattacgtt
     3481 tcaccttcag cagtgcgata agcaaatcga acagcgcgtc ggtaagcacc gccagaacaa
     3541 ttaccgggat cacccccagc aacactaaat caatggcgct gcttagcagc ccctggaaaa
     3601 ccagcgcacc aaaaccgcct gcgccgatta acgccgcaat caccgccata cctacagttt
     3661 gcaccatcac cacccgcagg ctgcgcagaa ataccggtaa cgccagtggt aactgaacat
     3721 gcaggaatcg ctgcgccccg ctcatgccca tcgctctggc gctctccagc acatcgcgcg
     3781 ggatctggtt caaaccgact accacgccgc gcaccagcgg cagcaaggca tagagcacca
     3841 gcgcaatcag tgcgggtgtc attccggttc ctgctatgcc gagcgtcccc agccacggaa
     3901 aggccgtaac cagcgcggca agcggcgcaa tcaacaggcc aaagagcgcc accgaaggca
     3961 cggtctgaat gacattgagc agagaaaaaa ttgccccctg ccgagcagtg gaaaagtagc
     4021 accagatgcc caacggcaca ccaatcacta acgcaggcag caccgcacca aacagcaacg
     4081 tcagatgttg tgccagcgcg tcgtcaaaca catcctgacg gttggcgtat tctttcatta
     4141 gtgagagatc gttaagcgtg ccggagtaca gcaaccacag cggaataatg gcaatctgca
     4201 tatgcaacaa ccagcgccac agcggatgcg tggagattcg gcggatggca tcactacagg
     4261 ccagcaatgc cagcgccgcg gccagccaga aaccactgcc gaggctggta cgcgccagcg
     4321 cactgccatt ttgcgccagt tgggtcgccg cctttcctgc tccccacacc agcaatacga
     4381 agacgaattg cgccagaatg agtgcaaaaa tgctgccttt tttagcggga ataaaacagg
     4441 ctgtcagcca ggcgcaacca acgcccacca gcatccagat cgtttgcggc cacagttgcc
     4501 agagatgacg cccttcaccc gagactaaac gattaggcgc gtaactgata aacggcagcg
     4561 ccgctgcgat tgccgtcagc aacagcagca gcgccagaac aggattaata cgtagataag
     4621 tcacgggaaa ttacttcgtc cacccttttt gtttcaggta gtcggcagcc acttttttgg
     4681 catccagtcc ttccactgca atgctggcat tcagttgctg caatgttttt gcatcgaggc
     4741 tggcgaagac tggctgtagc cactgtgcca tttgcggata ctctttcagc accgactcac
     4801 gcaccactgg tgcaggcgcg tagataggtt gcacgccttt cggatcgctt aaggtttgca
     4861 gccccagcgc cgcgaccgga ccgtcagtgc cgtaagccat tgcggcatta acgccagaag
     4921 tttgctgggc agcggcttta atcgttaccg ccgtgtcgcc gccagccagt gacagtaact
     4981 gatcctgatc gagtttaaag tcgtaggctt tttcaaacgc gggtaacgca tcggcgcgtt
     5041 cgataaactc tgccgaggct gccagtttga aggtgccgcc ctctttcaga taacgactca
     5101 ggtcggcaag cgaagtgagt ttgtttttct ctgccacatc ctgacgcacg gcgatggtcc
     5161 aggtgttatt cgctggtgcg ggcgtcagcc agattaactt gttttgctct gcatcgagtt
     5221 ttttgacttt ctcgtaacct tgctgcgcgt ttttccatgc cgcatcgttt tcatctttaa
     5281 agaagaaagc gccattaccg gtatattccg gatagatatc cagttcaccg gaagtaatcg
     5341 ccccgcgcac cacaggagtc gttccgagtt gcactttatt caccgtcggt acgccgtggc
     5401 tttccagcac ctgcaagatg atgttgccga gtagcgcgcc ttcggtatcg atttttgaac
     5461 cgactttaac gggggaagcc gcctgtagcg gcaggctcac ggctgccaac ataaccagtg
     5521 aacctgccca gacctttgag agtggcatga tgctttcctc attcttttac tgttgttttc
     5581 agcgaattaa gagaaaagca tagttgataa tgacagagtt agcctgagcg gaaggattca
     5641 gttgcagaat cagataaata cttaaagggc atattttgcc cggcgtttct tgccggatgc
     5701 ggcgtaaacg ccttatccgg cctacaaagg tcgaaaattc agcaagttaa gatatgaaca
     5761 tcaggcaaag ggcgcaaacg tcgcgccctt actaaagcat tacagcaact caaactcgcc
     5821 tttcttaacg cgtgcggaat cagtgccgat aaagacattg aacttgccag gttcggcgtc
     5881 atatttcatc tgttgattcc agaacttcag cgcctcgata tcaatcggga agctgacagt
     5941 ctgagtttcg cccggtttca gggtgatttt ctcaaagcct ttcagctgct tcactggacg
     6001 gctcatggaa gccgtcacat cctgcaagta catctgtact accgtggccc cttcgcgttt
     6061 accggtgttc gtcacctgca cgctggcggt cactttgccg tcacgcttca tggtcggcgc
     6121 agaaagtttc acatcagaga cggtgaaagt ggtatagctc agaccatagc cgaacggata
     6181 aagcgccccg ttagcttcat caaaataacg cgaagtgtat ttgttcggct tgtcggcatt
     6241 atacggacga ccggtattca gatggctgta gtacaccggg atctgcccga cagaacgcgg
     6301 gaaggacatt ggcagcttgc cggacgggtt gtaatcgcca aacaatacat cggcaattgc
     6361 attaccgcct tcagtccccg caaaccaggt ttccagaatc gcatcagcct gctgatcttc
     6421 tttcaccagc gccagcggac gcccgttcat cagcaccagc accagcggtt taccggtggc
     6481 tttcagcgcc gcaatcaagt cacgttggct ttgcggaata gtgatatcgg tacggctgga
     6541 ggcctcgtgc gccatcccct gtgcttcacc gaccacagcc accaccacat cagattgttt
     6601 cgccgtctgc accgcttcat caatcatctc ttgcggcgag cgcggatcga ctttgaccgc
     6661 ttcttcatac tgattcagga aatcgataat gcctttgtca ctggtaacgt tcgccccttt
     6721 ggcatacagc actttaccgt tttcaccgac ggcattttta atcccggtca gtacggtcac
     6781 ggattgatcg gcaacaccgg ctgcggacca gctgcccatc acgtcacgtt tactgtccgc
     6841 cagtggccca accaccgcaa tggtggccga ttttttcagc ggtaacgttt cgagacggtt
     6901 tttcagcaac accaggcttt cgcgcgccac ttcacgcgct tctttacggt gcaggcggct
     6961 ttcggcattg gtatccaccg ggtcagactc tttcggcccc aaatggctgt atgggtcgtt
     7021 aaacaatccc atatcatatt taacgttcag tacatggcgg gcagcatcgt ccagctcttc
     7081 catcgtcact ttgccggatt tgatcaaccc aggcagatac ttcgagtaat actcgtcgct
     7141 catactcatg ttgattccgg atttcagcgc cacgcgcacc gcatcttccg ggtctgccgc
     7201 cgtgccatgt ttaatcagct ctttgattgc accgtgatcg gaaacggtga tgcctttaaa
     7261 gccccactgg tcgcgcagaa catctttcag cagccaggaa tcggaggtgg ctggcgtgcc
     7321 gttcagcgag ttcagcgcca ccatcaccgc gccgctgcct gcgtccagcc ccgctttgta
     7381 cggcggcata taatcattaa acaggcgctg cggactcata tcgacggtgt tgtactcttt
     7441 accgccttct accgcgccgt atgcggcaaa gtgtttgacg ctggtcatca ccgagtagcg
     7501 atctgccggg cttttaccct gcatcgcttc caccatggtt ttacccattg ttgaggtgag
     7561 atacgtatct tcgccaaaac cttcggaagc acgtccccag cgcggatcgc gcgagacatc
     7621 gaccatcggt gcccaggtca tattcaggcc atcatctgcc gcttcataag cagagacacg
     7681 tccgaccgtt ttcactgcat cgaggttaaa agacgaggcc agaccgaggc taatcgggaa
     7741 caccgtgcgc tgaccgtgca gcacgtcgta agcaaagaaa agaggaattt tcaggcggct
     7801 taattccatc acctgatcct gcatggcgcg gatatcctga cgggttacgg tgttgaaaat
     7861 cgccccaacc tgaccgtctt tgatcatctc gcggatcgcc tctttcgggt tatccggacc
     7921 gacgctgatt aagcgcagct gaccaatttt ctcatcaact gtcattttct taagcagttc
     7981 ggtgacgaac gcatcccgcg cttcgggcgt taatggatgg ttgccgaata aatcatccgc
     8041 cagtgctggc tgcagggcca gactcaccgc gattcctact gaacatagcc atttcatatg
     8101 gatttttctt cctctgttgc ccgacgaggc agcgaaaatg gtgcaaaaac cgtagtttgc
     8161 cataagcatg atggagagag aaaaagaatg ctcagtttat tgtctgaatt ttcaaaatat
     8221 tcactcgctg aattgtcata caaggcgcta ttctagtttg tgatattttt tcgccaccac
     8281 aaggagtgga aaatgtcttc catgacaaca actgataata aagccttttt gaatgaactt
     8341 gcccgtctgg tcggtcattc acacctgctc accgatcccg caaaaacggc ccgctatcgc
     8401 aagggcttcc gttctggtca gggcgacgcg ctggctgtcg ttttccctgg ctcactactg
     8461 gaattgtggc gggtgctgaa agcctgtgtc accgccgaca aaattattct gatgcaggct
     8521 gccaatacag gcctgaccga aggatcgacg ccaaacggta acgactatga tcgcgatatc
     8581 gtgatcatca gcaccctgcg tctcgacaag ctgcacgttc ttggcaaggg cgaacaggtg
     8641 ctggcctatc cgggcaccac gctctattcg ctggaaaaag ccctcaaacc gctgggacgc
     8701 gaaccgcact cagtgattgg atcatcgtgt ataggcgcat cggtcatcgg cggtatttgt
     8761 aacaactccg gcggctcgct ggtgcaacgt ggcccggcgt ataccgaaat gtcgttattc
     8821 gcgcgtataa atgaagacgg caaactgacg ctggtgaacc atctggggat tgatctgggc
     8881 gaaacgccgg agcagatcct tagcaagctg gatgatgatc gcatcaaaga tgacgatgtg
     8941 cgtcacgatg gtcgtcacgc ccacgattat gactatgtcc accgcgttcg tgatattgaa
     9001 gccgacacgc ccgcacgtta taacgccgat cctgatcggt tatttgaatc ttctggttgc
     9061 gccgggaagc tggcggtctt tgctgtacgt ctagatacct tcgaagcgga aaaaaatcag
     9121 caggtgtttt atatcggcac caaccagccg gaagtgctga ccgaaatccg ccgtcatatt
     9181 ctggctaact tcgaaaatct gccggttgcc ggggaatata tacaccggga tatctacgat
     9241 attgcggaaa aatacggcaa agacaccttc ctgatgattg ataagttagg caccgacaag
     9301 atgccgttct tctttaatct caagggacgc accgatgcga tgctggagaa agtgaaattc
     9361 ttccgtccgc attttactga ccgtgcgatg caaaaattcg gtcacctgtt ccccagccat
     9421 ttaccgccgc gcatgaaaaa ctggcgcgat aaatacgagc atcatctgct gttaaaaatg
     9481 gcgggcgatg gcgtgggcga agccaaatcg tggctggtgg attatttcaa acaggccgaa
     9541 ggcgatttct ttgtctgtac gccggaggaa ggcagcaaag cgtttttaca ccgtttcgcc
     9601 gctgcgggcg cagcaattcg ttatcaggcg gtgcattccg atgaagtcga agacattctg
     9661 gcgttggata tcgctctgcg gcgtaacgac accgagtggt atgagcattt accgccggag
     9721 atcgacagcc agctggtgca caagctctat tacggccatt ttatgtgcta tgtcttccat
     9781 caggattaca tagtgaaaaa aggcgtggat gtgcatgcgt tgaaagaaca gatgctggaa
     9841 ctgctacagc agcgtggcgc gcaataccct gccgagcata acgtcggtca tttgtataaa
     9901 gcaccggaga cgttgcagaa gttttatcgc gagaacgatc cgaccaacag tatgaatccg
     9961 gggatcggta aaaccagtaa gcggaaaaac tggcaggaag tggagtaaaa attacggatg
    10021 gcatagtatc gccatccgtc attcacttaa tcgttctgtg ccgtctgccc cgccgccgcc
    10081 atttgggcgg ctttttgttt tttatagctc aacgctgctg ctggaacagg catcacttta
    10141 ccggtttcaa tccaggtacg caggcggctg gcatcggcaa aatgggtata tttgccaaac
    10201 gcatccatca ctaccagcgc caccggttta ttattgataa ccgtacgcat caccagacaa
    10261 tggcccgccg cattggtaaa accggttttg gttaactgaa tattccagtt atcgcgatac
    10321 accagatggt tagtattgcg gaacggcagc gtatacgtcg ggttagagaa ggttgccata
    10381 tcttcccggg tagtacttaa ctgcccgatc aacggatatt gtttgctggc aatgagcagt
    10441 ttggttaagt cacgggcagt tgaaacgtta tgcaccgaca atccggtagg ttcaacaaag
    10501 cgcgtgctgt tcattccgag cgatttcgct ttcgcattca ttgccttaat aaaggctttg
    10561 taaccaccgg gataatggtg tgcaaggctt gccgccgcgc ggttttctga agacatcagc
    10621 gccagcaaca gcatatcttt acggctgatt tcgctattca gtcgtacgcg cgaatagacc
    10681 cctttcatct ccggcgtctg gctgatatcc acttttagtt tttcatccag cggcagtcgt
    10741 gcatccagca caaccatcgc ggtcattaat ttgctgatag acgcaatcgg acgcaccaga
    10801 tccgggtggt tcgagtagat cactttgttg gtattcagat caacaatcat cgcgctaccg
    10861 gaggcaattt caggttgtga agcggtggta gcggctgccg ttttcgcaac ggcctgcggt
    10921 gccaaaggca cagccagcat cagggccagg ctaaataaag aaactcgaaa tttcggcatg
    10981 atgagcattc agatagtggt tcacgcgcac aggttgcgca ccgccggagt aaggatttac
    11041 tgaggctagc gacgccatca taacgagcaa aaagtgcgat cgtcaaagga gaatcgtgag
    11101 gaaatgctgc attgctgaca tttacgccag caatgcaacg tcaaagaact ttctagaaca
    11161 aacgataacc gtagccccat agtataacgg ttagggcgag cagcacttcc agtaccagca
    11221 cgccaatcgc cagcgtcgaa ctagagaagc taaggccttc ctctttgttg atattcagga
    11281 agctcggaat accaaggtaa agcaggtagc cggtgtaaaa cagcgccacc gtgccgacca
    11341 gcgcacacaa ccagaccagt ggataaagcg ccaccagacc gcttaaaaac agcggagttg
    11401 caacgtagcc cgcgaagacc atgcagtgcg caagtgacgg acgctgcgga taattacgcg
    11461 ccatccacca gatgacccgc cccattaccg caaccccagc cagcataacg ccataaaaca
    11521 ggacagccag cgccagtccg gtaaaccagg ataacttcag gatagtgcca tcgccaaaat
    11581 tccagccaat ctgtgtagtg ccaatgaagg cgcaaatcac cggaatcgcc gccatcagca
    11641 aaacgtggtg ggtgtaatgg tgagaaatcg tttcgttttc gcgattaatc acctgcattt
    11701 cacgatcggg atgggaaaac agtccccaga catggctcat accgccccct tgttgtgagt
    11761 tcatgaacct gacagttcaa gtataagtca gcttgtgatt attttttgtt cgctcatgta
    11821 atttgcaccg tctgatgtac gtcctttttc agcgtgaatg atttccaggg tgtatgctta
    11881 caggcaacca aagggagaca gactggccta tggatctcaa tacactcatc tcacaatatg
    11941 gttatgccgc gctggtgatc ggtagcctgg cggaaggtga aaccgtgact ttgctgggag
    12001 gcgttgcggc acatcagggg ctattaaagt tcccgctggt ggtactttct gtggcgcttg
    12061 gcggcatgat tggcgaccag gtgctttatc tgtgcgggcg gcggtttggc ggcaagctgt
    12121 tacgccgttt ctcgaaacat caggataaaa ttgagcgggc gcagaaactt atccaacgcc
    12181 atccgtatct gtttgtcatt ggtacgcgct ttatgtatgg ctttcgggtg attggcccga
    12241 cgctgattgg tgccagccag ctgccgccga aaatctttct gccgctgaat attctcggcg
    12301 catttgcctg ggcgttgatt tttaccacta ttggttacgc tggtggtcag gtgattgcgc
    12361 cgtggttgca caatctcgac cagcatttga agcactgggt ctggttgatt ctggtcgtgg
    12421 ttctggtggt gggcgtgcgc tggtggctga aacggcgcgg gaagaaaaag ccggatcatc
    12481 aggcgtaaaa ccattgccct gggtaaggcg ttcgcgccgc atccgacatc cggtgcacca
    12541 tgcctgatgc gacgcttgtg cgtcttatca tgcctaccaa aattttaccc attgaaataa
    12601 ccacgcacct cactgcgcta ttctgggttg aactgcggat tcgccaacat aaagccgcca
    12661 tccactatca acgactgccc ggtggtgtaa tttgcgccct ctgagcacag ccacgctacc
    12721 aggctggcaa tctcatgcgt tgcgccaaaa cgccgcagag gaatcgaagg ctccgcgtcg
    12781 ggcttcacgt cgctgtcatc catgccattc attggcgtgg cgatcgcccc aggcgcgact
    12841 gcgttcacca aaatcttatg cctgaccagc tccagcgcca tcgttttggt taacccgccg
    12901 agcgcatgtt tagcggctgt gtaagcgctg gcatccggca gcggcgtatg ttcatgtacc
    12961 gacgtaatgt tgatgatacg accgccctgc ccttgtttca ccatctggcg ggccgcaatt
    13021 tgtgagcata aaaatgcacc atcgacatca acggtaaaaa tcttgcgcca ctcatcgaaa
    13081 gccatatcaa gaaacggcgc tttggtcatt gcgcccgcat tattcaccag cacatcaata
    13141 cgccccagcc gttgaatgag tttctccagc gcctgcgcac cttctggcag tttaccgagc
    13201 tccagctgca cgatctccgc acgtacgccg tggctaacta cctcacgcgc ggtatctttt
    13261 gccccttctt catctgagtg ccaggtaatg ccaatatcaa acccctgctg cgccagtaat
    13321 aacgcgcact ctttgccgat ccccgaatcg gaggcggtaa taatcgcaac ctgtgccatc
    13381 gagttctcca cttaacgctg aataaacgtt aagtatagaa ggcgcatatc atcagcgttt
    13441 gtaacccccg cccaacgcac cagtgagttg aatagaagca tccagccact gcccttgcaa
    13501 taacaggcca ttggcccgct cacgcagcgc ggggattctg gcttcgctga cgcgggaacc
    13561 agcaatgatg cccgcgttaa agcgcgcctg cgcaagacct accacacgca aggcatcgcg
    13621 ctcaatttgc gcctgatgct ggtttttctc cgcaagtgtc tgaacctgac tggctgcccg
    13681 cgccacgtca ttcaccgctt caaccaccgc tttgttgtag ctggcgatag acaagttgct
    13741 ttcggctttt gcgatatcga gattggcgtt aagacgacca ctatcgaaaa tgggtagcgt
    13801 caggcctgcc gtaacgccca tttgctgcgc ggaatgacgg aacagatcgc ttaagtgcaa
    13861 cgcatcctgt tgcaggaagg ccatcaggtt gatgtcagga taaaatgccg cttttgccgc
    13921 atcaatggtg cttagcgatg actcaacgta ccagtgcgcc gcctgcaaat ctgcccgccg
    13981 ggccagtaag gagtacccca gttcatcagg aagctggctt gccactttcg gcaacgcgac
    14041 cggtttaagc ttcaatgact ttgtctggtt atttgtaagt gcgcttaacc gtgcctcaat
    14101 aattttcatt ttccccgcga catcgttgag ctgctgccgg gttttgctgg cattaatatc
    14161 ggtttccaca ccttcaactg aagaagtaat cccgttctga tatagctggc gatcggtcgc
    14221 gataatggtg ttctgctctt tttctatttg ctgcaagacc gtgtttaacg ccgcctgggt
    14281 ttgccactcc cagtacaggc gggctacgct gccagccagc aattggcggg tttgctcgcg
    14341 ttccgccgcc cgtgctttaa ccgtacccag gcgggcagta acctccgccc gattctttcc
    14401 ccagatatcg agatgccagc ccgccgttaa gccaaaagta ccgttggtgt accacgggcc
    14461 ggtcgtacct gcggccggat cgttcagagc aaacggcccc attaagcctt ctgccgacat
    14521 tttttgccgc tccatatccg ccgaaaagtc gatctgcgga ccatcctgag tggcaactgc
    14581 cttcgcctgg gcttcagcta gctgaatgcg ctgttcagcc acctgcatat ccggtgcgtt
    14641 ctgtagtgca ttgttaatta atgaagtgag ctgattatcg tgatactcca gccaccattg
    14701 gctgtctggc caaccatttt tcagcgccgt gggtaatgcg gtgtcaactt gtgcagcggg
    14761 cgtttgctgg cttaacgcct ggcgggtttc atgcataggc gcacacccgg ccagcatcag
    14821 taacagcgga aaacaggcga tggctggata aaaggaatca cgattcatgg gggaataatc
    14881 aggtaagaaa cggtgcgcgg agattaccgt gtgttgcgat atatttttta gtttcgcgtg
    14941 gcaatacatc agtagcaata aaacgacata cccagaaaaa tatagactaa gtaaatgata
    15001 tcttccgatt tatcttaatc gtttatggat aacggcaaag ggcttcgttt tttcctatac
    15061 ttattcagca ctcacaaata aaggaacgcc aatgaaaatt atactctggg ctgtattgat
    15121 tattttcctg attgggctac tggtggtgac tggcgtattt aagatgatat tttaaaatta
    15181 attaatgtca tcaggtccga aaataacgag aatatttcag tctctcatcc tgttgcgctc
    15241 ctgtcatgtg cattgcttca tataatcact ggcgcaagga gcgcgcaggg ggcggccaat
    15301 cgccgccgcc ccctgcaccc ccgggctctg gcgaacaaaa tcgccgctgc gcggtgccct
    15361 cggcttatcc cttacggcta ccgggtcggg cgcgaggtaa catccctgta aaacgcgccc
    15421 tcagcccaca tccatgtggg ctgccccggc cttcagggaa cgcctcggca attttgacgc
    15481 caccaaacaa ccgtgcggcc tattgataaa gagctaacac attgtcaaaa aacatcacta
    15541 tggtttttta gagtttctcg atatcaattg cctgaatagc ccttgcaata tcaggggaat
    15601 tattcaacac ccgaacatgc tgaaataatt ccgttgcttc atcgtattct ttacgcaaat
    15661 aactcaacca ctgtttaatc cgcgcaacgt gatataaccc ggtatcgccc tgcttttcca
    15721 gacgggtata tttttgcagc aaagcaacca cctccggcca cggcattcgc ggttcgttat
    15781 attttactac ccggctcagg ttgggaatat tgagcgcccc gcgaccaatc atcactgcgt
    15841 cgcagccgct gatcgcaatg cattgttgcg cgctctgcca gtcccagatt tcgccgttgg
    15901 caatcaccgg aatattcagc cgctggcgaa tctcgccaat cgcctgccag tcaatatgct
    15961 ccgcgcggta accctgctct tttgtccgtc catgcaccac cagctccgta gcaccagcct
    16021 gttgaaccgc atcggcgatt tcaaatttct tctcaccgct gtcccagccc agacgcactt
    16081 tcacgctgac gggcaaatgc gccggtacag cttcacgcat cgcttttgca ccctggtaga
    16141 tgagttcagg atctttgagt aacgtcgccc cgccgccgct gccgttaacc gttttcgacg
    16201 ggcagccgca attgagatcc acgccccagg aacctaattc caccgcacgg gcggcgttct
    16261 ctgccagcca ttgtgggaac tgacctaaca actgaacgcg caccagcgta ccggatggtg
    16321 tccggctggc gttttgtagc tcagggcaaa tgcgatgaaa gacttttacc ggcagcagtt
    16381 gatccaccac gcggacaaac tcggtgatgc acagatcgta gtcgttaact tcggtcagca
    16441 attctcgcac cagagagtca agcacaccct ccatcggagc cagtaacaca cgcatatctt
    16501 cacccggaaa aaaatgaggc gctatgttag cgcctatggt caacggatta aaggtctgca
    16561 aagagggatt gctgcaaact gtaatccctc tctatgcttg atgtatgcct gagttcgttg
    16621 cgcattgcgt aatgccgggg agcgaaaaaa taagcgctga tttcttaatg tgatcggtag
    16681 cacgttttac gaattaattg tatgatgaat ccatctcatc tggggtgttg attatgagta
    16741 agacactgaa cattatctgg caatatttac gcgctttcgt cctgatttat gcctgcctgt
    16801 atgcaggcat tttcattgct tccctgctac cggtaaccat tccgggcagc atcatcggga
    16861 tgctgatcct gtttgtcctg ctggccttgc aaattcttcc ggcaaaatgg gtcaatccgg
    16921 ggtgctacgt actgattcgc tatatggcgc tattgtttgt gccgattggc gtaggcgtca
    16981 tgcaatattt tgatttgctc cgcgcacagt ttggcccggt agtggtttcc tgtgcagtca
    17041 gtacgctggt ggtttttctg gtggtgagct ggagttcaca actggtacac ggtgaacgta
    17101 aagtcgtagg tcagaaagga tcagaagaat gatggcgaat atctggtggt cattaccgct
    17161 gacgttgatt gtcttttttg ccgcccgcaa actggcggca cggtataagt ttcccctgct
    17221 taacccgttg ctggtagcaa tggtggtgat cattcctttt ttaatgctga ccggcatctc
    17281 ttacgacagc tactttaaag gtagcgaagt gctaaacgat ctgctgcaac cggcggtcgt
    17341 cgcgctggcc tatcctttat atgagcagct acaccagatc cgcgcgcgct ggaaatcgat
    17401 catcaccatc tgttttatcg gcagcgtggt tgcgatggtg acggggactt ccgtggcatt
    17461 gttgatgggc gcttcaccgg aaatcgccgc gtcaatcctg ccgaaatcag tcaccacgcc
    17521 tattgcaatg gcggttggcg gcagtattgg cggtattccg gcaatcagcg cagtttgcgt
    17581 gattttcgtc ggcatcctcg gcgcggtatt tggccataca ttgcttaatg cgatgcgtat
    17641 tcgtaccaaa gctgcgcgcg gtctggcaat ggggacagcc tcgcacgccc tcggtacggc
    17701 gcgctgcgcc gagctggatt atcaggaagg tgcatttagt tcgctggcgc tggtgttatg
    17761 cgggataatt acttcgctga tcgcaccgtt ccttttcccg attattctgg cagtaatggg
    17821 ctaaaatttg cgatgcgtcg cgcatttttg atgtaagttt cacgcgttgc ataattaatg
    17881 agattcagat cacatataaa gccataacgg gttcgtaaac tgttatccca ttacatgatt
    17941 atgaggcaac gccatgcatc cacgttttca aaccgctttt gcccaacttg cggataactt
    18001 gcaatctgca ctggaaccta ttctggcaga caagtacttc cccgctttgt tgaccgggga
    18061 gcaagtctca tcgctgaaga gcgcaacggg gctggacgaa gacgcgctgg cattcgcact
    18121 acttccactg gcggcggcct gtgcgcgtac gccattgtcg aattttaatg ttggcgcaat
    18181 tgcgcgcggt gtgagcggaa cctggtattt cggtgccaat atggaattta ttggtgcaac
    18241 aatgcagcaa accgttcatg ccgaacaaag cgcgatcagc cacgcctggt tgagtggtga
    18301 aaaagcgctt gcagccatca ccgttaacta tacgccttgt ggtcactgcc gtcagtttat
    18361 gaatgaactg aacagcggtc tggatctgcg tattcatctg ccgggccgcg aggcacacgc
    18421 gctgcgtgac tatctgccgg atgcctttgg gccgaaagat ctggagatta aaacgctgct
    18481 gatggacgaa caggatcacg gctatgcgct gacgggtgat gcgctttctc aggcagcgat
    18541 tgcggcggca aaccgttcgc acatgcctta cagtaagtcg ccaagcggtg tcgcgctgga
    18601 atgtaaagac ggtcgtattt tcagcggcag ctacgctgaa aacgccgcat tcaacccgac
    18661 tctgccaccg ttgcaggggg cgttaattct gttgaatctc aagggttatg attacccgga
    18721 tatccagcgc gcggttctgg cagaaaaagc cgatgcgccg ttgattcagt gggatgccac
    18781 ctccgcaacg ctgaaagctc tcggctgtca cagtatcgac cgcgtgcttc tcgcttaagc
    18841 ctggtgccgg atgcggcgtg aacgccttgt ccggcttgcc agccctctcc tggtgtcgaa
    18901 attcccggca aacagtttgc cgtttcttgc gcaaaaccag cgggtaaagt agcctgatgg
    18961 aaattttcct tagatcgagt ctcctgcatg ttaaagcgcg tgttcctcag cctgttagtc
    19021 ctgatcggct tgctgctgtt gactgtgctc ggcctcgatc gctggatgag ctggaaaacc
    19081 gcgccttata tctacgacga attgcaggat ctcccctacc gccaggtcgg cgtggtgctc
    19141 ggcacagcaa aatattatcg tactggcgta attaatcagt attatcgcta ccgcattcaa
    19201 ggagcgatta atgcctataa cagcggtaag gtaaattatc tattactgag cggcgataac
    19261 gcattgcaaa gttataatga gccgatgacc atgcgcaaag atttaatcgc tgctggtgtc
    19321 gacccatcag atattgttct cgattacgca ggctttcgta cgctggattc catcgtgcgt
    19381 acacgcaaag tgttcgatac taatgatttc attattatca cccaacgttt ccactgtgag
    19441 cgggcattat ttattgcgct gcatatgggg attcaggctc agtgttatgc cgtaccgtca
    19501 ccgaaagata tgctgtcagt acgtattcgc gaatttgccg cccgtttcgg tgcgctggct
    19561 gacctttata tttttagacg tgaaccgcgt tttttagggc cgctggtccc tattccggct
    19621 atgcaccagg tgccggaaga tgcgcagggg tatcccgccg tcacacccga acagttactt
    19681 gaattacaaa agaaacaagg aaagtagtta tggatgtaca gcagtttttt gtcgttgccg
    19741 tttttttcct tatcccgata ttttgtttcc gcgaagcatg gaaaggctgg cgcgcaggcg
    19801 cgattgataa acgggttaaa aatgcaccgg aaccggtgta tgtctggcga gcaaaaaatc
    19861 ccggactctt tttcgcttat atggtggcat atatcggctt cggaatttta tctatcggca
    19921 tgattgttta tcttattttc tatcgttaat attttctctt ttctgttaat tattgagaat
    19981 aattattact tcactcaata agctgcggat ataacattct gaaccgcagc tatttgtgaa
    20041 tcctttcaca gtttaaattt ccccacacgc ttagccttaa tatcagcacg caatatttat
    20101 ttagcgttcg ccttaattac tcatagcatt aaggaagatc acatgccgca acaaaattat
    20161 ctggatgaac tcactccggc ttttacgcct ttactggcga ttaaagaagc ctctcgctgt
    20221 ttattatgtc atgacgctcc ctgtagtcag gcttgcccgg cacagaccga tccggggaaa
    20281 tttattcgct caatctactt tcgtaatttt aaaggcgctg ccgagacaat tcgcgaaaat
    20341 aatgccctcg gtgccgtttg tgccagagtg tgcccgacgg agaaattatg ccaaagcggt
    20401 tgtacccgtg ccggtgttga tgctcccatt gatatcggcc gcttacagcg ttttgttact
    20461 gattttgaac aacaaaccgg gatggaaatt tatcagcccg gtactaaaac gctcggcaaa
    20521 gtcgcgatta ttggcgcagg tcctgccgga ttgcaggcca gtgtgacact gacaaaccag
    20581 ggttatgacg tcacgatcta tgagaaagaa gcacaacccg gtggctgatt gcgtaacggc
    20641 attccgcaat tccggttacc gcaatcagta ctggatgcag agatcgcccg tattgaaaaa
    20701 atgggcgtga ccattaagtg caacaacgaa gtcggtaaca cactcaccct tgagcagctg
    20761 aaagcagaaa accgcgcggt actggtcacc gtggggttat caagcggttc cgggctaccg
    20821 ctgtttgagc atagtgacgt tgagattgcc gtcgacttct tgcaacgtgc acgacaggcg
    20881 caaggcgata tcagcattcc acaaagcgca ttaattatcg gcggcggtga tgtcgcgatg
    20941 gacgtagcca gcacgctgaa agttctcggc tgtcaggcgg taacttgcgt agcgcgtgaa
    21001 gagttagatg agtttccggc aagcgaaaaa gagtttacca gcgcccggga gctgggcgtt
    21061 tcgatcatcg atggattcac gccagtagcc gtggaaggca ataaagtcac gtttaagcat
    21121 gtacggttat cgggcgaact gacgatggcg gcagataaaa ttattctcgc cgtcggtcag
    21181 catgccagac tagatgcctt tgcggagtta gagccgcagc gtaacaccat caaaacacaa
    21241 aattaccaga cccgcgaccc gcaagtcttt gctgctggcg atattgttga gggagacaaa
    21301 accgtggtct atgccgtgaa aaccgggaaa gaagccgccg aggcgattca tcactattta
    21361 gagggagctt gctcatgtta acgaaagatc tttcgattac tttttgcggc gtgaagtttc
    21421 ccaacccgtt ctgcctctct tcttcgccgg taggcaactg ctatgagatg tgtgccaaag
    21481 cctacgacac aggttggggc ggtgtggtgt ttaaaacgat cggctttttt atcgccaacg
    21541 aagtctcgcc gcgttttgat catctggtga aagaagatac cggttttatc ggcttcaaaa
    21601 atatggagca gattgccgag catccgctgg aggagaatct ggccgccctg cgtcggctga
    21661 aagaagatta cccggacaaa gtattgatcg cttcgatcat gggggaaaat gagcagcaat
    21721 gggaggagct ggcgcgcctg gtgcaagaag ctggcgcgga tatgatcgag tgtaacttct
    21781 cctgtccgca aatgacttct catgcgatgg gtagcgatgt cgggcaaagc ccggagctgg
    21841 tagaaaaata ttgtcgggca gtgaaacggg gttccacgct gccaatgctg gcgaagatga
    21901 cgccgaatat cggtgatatg tgcgaagtgg cgctggcggc gaagcgcggc ggcgcagatg
    21961 gcattgcggc gattaacacc gttaaatcca tcaccaatat cgatctcaat cagaaaatcg
    22021 gtatgccgat cgttaacgga aaatcgagta tttccggata ttccggtaaa gcggtaaaac
    22081 cgatcgccct gcgcttcatt cagcaaatgc gcacccatcc agaactgcgc gatttcccaa
    22141 tcagcggtat cggcggcatt gaaacctggg aggatgcggc ggagttttta ttgctcggcg
    22201 cagcaacgtt acaggtcacc accggcatta tgcagtacgg ctatcgcatc gtagaagata
    22261 tggcgagcgg gttatcgcat tatctcgccg atcagggatt tgattcgttg caggagatgg
    22321 taggtctggc gaataacaat attgtcccgg cggaagattt agaccgcagt tatattgtct
    22381 atccccgtat caatcttgac aaatgtgttg gctgtggacg ctgttatatt tcctgttacg
    22441 acggcggtca ccaggcgatg gaatggagcg agaaaacccg cacaccgcat tgtaataccg
    22501 agaaatgtgt gggttgtctg ctttgtggtc acgtctgccc ggtgggttgt attgagctcg
    22561 gggaagtgaa gtttaagaaa ggcgagaaag aacacccggt aacgttgtag aacccgcgtc
    22621 acatccggca tttggttcct gttgccggat gcggcgtgaa cgccttatcc ggcctacaaa
    22681 accacacaga tcatgatgtt atgtgcgttt atgaccgaat gcggaccaca ttcacatcat
    22741 ttcttacgcg cgtatttcag tgaatccagc gctacggcga agataataat cgcccctttg
    22801 atgatgtact gccagtatgg gtttacgccg atatacgtca ggccatagtt gatgacggta
    22861 aaaataatca ccccggtcac cacgccaatc accgtcccca caccgccgct gaacgataca
    22921 ccgcctacca cgcacgccgc gatagcatcc agctcataca taaagccgag gttgttggtg
    22981 gcagagccga tacgtccggc ttctaacatc ccgccaaagg catagaacac gccagacaac
    23041 gcgtagatca tcagcaggtt caggccgacg ttgacaccag atacttttgc cgcttccggg
    23101 ttaccgccaa tggcaaaaat gttcttaccg aagcgggttt tgttccacaa cacccagacg
    23161 aacgccaccg caatcaacgc gtagaaggtg atgtaagaga gacggaaact ccccagcgcg
    23221 acaaagccct gagcaaaggt agagaagcca ctgtcaaaac cagaaattgg cgacgccccg
    23281 acaaagtcgt aatagagcga gttgatgcca tagacgatga tcatcgtgcc gagcgtggta
    23341 atgaacggcg tcacgttgag ataagcgata atcagaccgt tgatcaaacc gatcaccgca
    23401 ccaatggcac agacaatcag aataaccagc gcaatcggca tcgtcgccat ttccgggaac
    23461 actttgttgg cgttatccat ggactgcaat aatgtcgcag ccaccactgc cgccagccct
    23521 acctgacgac cagcagaaag atcggtcccc tgggtgacaa ttaaccctgc cacaccgagc
    23581 gcgataataa tacgcaccga tgactgggtg agaatattac ttaagttcaa cagacttaaa
    23641 aatgttgggt cctggaaaat aataatcgcc agcaaaacta aaagaacgac gtaaataccg
    23701 ccctctttca ggtaagtaag aaaacttttc ttatttaacg cactcatggg aagcccctaa
    23761 tcttaaaggt gcaaagacgc aagacgcaga atttcgtttt gcgttgttgt ttttgtatcg
    23821 acaattccgg aaacgagacc attgctcatg accagaatac ggtctgttat ccctaacaac
    23881 tcaggcattt cagaggagat aataataatc cccttgcctt tcttcgccag ttcggcaatt
    23941 aactgataaa tttcaaactt cgccccgaca tcaataccgc gcgtcggttc atcgagcatt
    24001 aatatttctg gttgcgttag tagccagcga ccaataatca ccttttgctg attaccaccg
    24061 gagagcgaac caatttgcgt ccgatgaccc ggcgttttta cccgcatcga atcaatcacc
    24121 cactgggtat cgcttttcat ccgcgagtta tccagtaaac caactttatt tttgtagttg
    24181 cgaatattgg aaattaacga gttaaaacca atatccagat aggcataaat tcccgttgag
    24241 cggcgctcct cagttaccag tgcaaatccg tggtttatgg cttcgttggc attatggtta
    24301 ttgatctgtt tgccgtgcaa cgtaatggtg ccagccgatt tctcgcgaat accaaataac
    24361 gtctcaacaa tatcggtacg tttcgccccc accagcccgg caataccgag gatctcccct
    24421 ttatgcagat caaacgagac atcgcgaatc gacggctggc gcagtgacgt taggttacgc
    24481 acctcgagga tgacttcgcc cggcttgttt tctttgtcag gaaaacgctg attaagagaa
    24541 cgcccaacca tcatggcgat gatcttgtcc atcgtcagtc ctgccagcgg ttcggtggcg
    24601 atccactgac cgtcgcgcaa tacggtaact tcatcacata actggaagat ttcttccatt
    24661 ttatgcgaga tataaacaat accgcagccg cgctctttta atttacgaat aatagtgaac
    24721 agatgattga cctctttttc ggttaacgaa gaagtcggtt catccataat cacaattttc
    24781 gcgttatagg aaaacgcttt ggcgatttcg atcatctgca tttgcgaaac ggataatgtg
    24841 ccgacgcgcg cacgcggatc gatatcaata tccagttcat caaaaatcgc tttggtttcg
    24901 cggtacattt tgtcctgatc gacaaacatg cctttggtgg gatatcgccc cagccacatg
    24961 ttatccatca ccgaacgttg taataccagg tttaactcct ggtgtaccat cgaaatacca
    25021 ttttccagtg cctctttggc ggaatggaaa tcgatctctt taccctggaa taaaatggtg
    25081 ccggagtctt tttgataaat accaaacagg cattttaata atgtcgattt tcctgcaccg
    25141 ttttccccca ttaatgcatg gatagaatgt ggccggactt ttaaattaac gttatcaagt
    25201 gccttaacac caggaaagga cttgttgata ccgctcattt ccaacaagta ttccccggaa
    25261 gacggagtcg ttgagctgac catataattt taccttgttg gccatacaat aagggcgcag
    25321 taaacgactg cgcccaatca gtcttatttc ttgctgaatt cagccaggtt gtctttatct
    25381 acgccaacat aaggtacgcg gaccactttg ttgtcgattt tccagttggt gccatcagcc
    25441 gcacctttgc catcggccag gtttttcgcc agatcaaagg tcgctttcgc ctggttgtta
    25501 gcatcgttca gtactgtgcc cgccagtgca ccggatttca ccagcgccag cgcttctggc
    25561 agcgcatcga cgccaaacac cggaatgctg gacttgttgt gtgctttcag tgcttctacc
    25621 gcgcccattg ccatcgcatc gttgttggcg ataaccactt cgattttgtt ggcgttcggg
    25681 ccagacagcc aggcgtccat cttatctttc gcctgagcgg tgtcccacat tgcggtatct
    25741 aactgtaact gttcagtttt gatgccttta tcgttcaatt ctttaatcac gtaagtggta
    25801 cgtgcttctg catccggatg gcccggttca cctttcagca gtacgaactg aatctgaccg
    25861 tctttgttca gatcccaacc ctgattcgcc gcccagtgtt tagcaatcaa atcgccctga
    25921 ataatgccgg actctttgga gtcagtgcca acgtagtagg ctttgtcgta gctatccagc
    25981 gccttacgag acggttcttt gttgaagaaa accaccggca cgttttgccc acgcgctttc
    26041 tcaatcaccg tacccgcagc tgccgggtca accaggttga ttgccagtgc cttcacccct
    26101 ttcgccagca atacgtcgat ctgatcgttc tgcttggact ggtcattctg agaatcattc
    26161 atcagcagct gaacatctgg cgcggctttc gcatcttgct caatagcctt gcgcactaca
    26221 gacataaagt tatcgtcgta cttatagatt gttacaccaa tgcgagtatc agcagcgtgt
    26281 gcagcggcac cgaataacat gctggccatc acagcagaca gggttaacac cttcttattc
    26341 atggtatctc cggtttttct tatgcagggt agtgcttgag ataaatgctc ggcggggcag
    26401 tagagttaat gaagtgttac tgaacgccga agctcacttt tttaaattcg ttcttccatg
    26461 cccggtaacg ctccagaaaa cggctttaat tgttgttatg acgctattac ctcggtgaaa
    26521 gtgattattc actgttacat acgggttaca acgttaaaac ggtgcaatca tagctatcac
    26581 attgttaaga tactgtgaaa tcactcacag attgaaagcg gttacatcgc ctgatttgtt
    26641 gagttagtga tcgccgccgc attctggcgc gttgcgacag aatggcgacg cactaacgtc
    26701 ggcataaaac agtggctggc acgaggatca atattgcctg ctgccccctg caaggccagt
    26761 tcggtggcta atttagccat tgaagcaatg ggataacgca cggtcgttaa ttgcgggtcg
    26821 gtgtaacggg cgatgggaat atcatcgaaa ccgatgattg agagatgtaa cggaatcgca
    26881 atgccattat cttttaatgc tgtcagtgcg ccagcggcca tattgtcgtt ataagcaaat
    26941 atagcggtaa gttgtagatt gcgccccagc agttcaacca tcgccgcctc accgcccggc
    27001 atgtccggcg taccagtgcc aatccagctt tccggcggaa taatatcctg ctctttcaac
    27061 gcactcatcc agcctgcttt acgcatggcg tcatcttcaa tgccgtgact ggaagaaaga
    27121 taaccaatac gttgatgacc gttattcagc aacatgcgcg tcgccattcg ggcaccgctg
    27181 agattatcca ggcaaacgca acgatgggcg taccccggca caacgcggtt gattaacacc
    27241 ataccgggaa tgttatccat aaattgcgcc agttcatcgt cactcaatgc ttttgagtga
    27301 acaatcaacg cattacaacg ctggcgaatt aacacctcaa tggcgtgacg ctctttttcc
    27361 gcttcatgat agctattgcc gattagcacg tatttctgat gctgctgagc gactagatcc
    27421 accgctttta ccagcgcgcc gaaaaacgca tcagaaacgt ccatcaccac cacgccaatg
    27481 gtgtcgctaa cctgagttgc cagcgcctgg gcattggcgt ttggccgata atccagctca
    27541 ctcacggctt tcattactgc ttcacgcgtg tcggcactga ccagcgtgct gttattgagc
    27601 acccgggaaa ccgttgccac agagacgcca gcctaacgcg ctacatcacg aatggtgatc
    27661 atattcacta cctgttatga gattgcagta aatgactgct tgctggcggc tattttgtca
    27721 gcactgaata caggacttcg tgaatcgagt cacagcaatg gaaacggtta cagccgtttt
    27781 gttaacgaat gtgatccaga tcgttatctt gatgttttag atattgccgt tccggcagca
    27841 cgcaaagtta actgacgcca gagccattcc accggcccct ggcggaagta acgcagccag
    27901 ataacagaga agaggatatt cgccagccat accggaataa caaacgccag cagctccagg
    27961 cggtcaaaat gcataaacaa accgaggtgg taaaaaagca tggtacaaat cagcgtttgc
    28021 aatagatagt tggtcagcgc catccgtccg acgcaggcga tcgcaagcac cagcttaaag
    28081 cggctcaatt gcggccagaa gccataaaac agcgacgcat agccaatcgc ctgaaacggc
    28141 gcactcagtt cccgcggcat ttgaagtaag aaggcacacc agcgatacgc ccagtccagc
    28201 tgccattgca gggcgatggc aggaaggtta atggtcaccc caatcgccac cagcacaaaa
    28261 ccagtacgac gatagtgacg taagctgaac tgccctttca gccagccgct gcgcatcagc
    28321 gccgcaccaa tgagcatcat ccctgccagt tgccagccat actgtgcgcc cagtgccagt
    28381 aaactgttgc caacaccatc ggcacgatta ctgatcgctt caacgccgcc gtgaagcttc
    28441 cagtattttt catacaaaat agccgatgca tccggcgtcc aggtgcggct ggtctggctg
    28501 tcggaaatca gccccaataa cagcaaaacg ccaagcccca ccagataaag catgacgcct
    28561 gtattaaaca ggctttttac cgatggcgca tcgcgcacca gccgccagca gattaagccc
    28621 accagcccgt aagccagcag aatatcgccg tcccagaaca ataaaccgtg aataaaaccc
    28681 agcaagacta acagcgttaa ccgcgactgg atccagcgtc tgccacgggg cagcaacatt
    28741 tgcaggcccg caccaaacag cagcgcaaaa agcgtgagga atttcacctg gccgatgaga
    28801 tcgagaaatg cccaggtcca tgcatcctgc ggcgtaatag cgccgtacca ggcgggattg
    28861 agataagccg cctttggtag cccaaaggcg ctgatgttta atagcaggat ccccagaatg
    28921 gcgacgccgc gaacaaaatc gagcgttacg ttgcgctcca tggttcctgc cttttaatca
    28981 gttgtgatga cgcacagcgc gcagaaactc gtggcgcgta ttctgactgg atttgaacaa
    29041 tccaccaaga gaggtcgttg ttgtggcact ggttgcatcg cggatgccac gcgctttcac
    29101 gcagtaatgc accgcatcaa tggagacagc cacgttattg gtgcccagca gcgtttgtag
    29161 cgcaataaga atttgctgcg tcagacgttc ctgcacctgc ggacgctggg caaagaactg
    29221 cacgatacgg ttaatttttg acagaccgat caccgaatct ttcgggatat aagccaccgt
    29281 cgctttgcca tcgatggtaa caaaatggtg ttcacaggta ctggtcagag tgatatcgcg
    29341 cacggtgacc atttcatcga ccttcatttt gttttcaatg agggtgattt tcgggaagtt
    29401 ggcgtaatcc agaccggaga aaatttcatc gacatacatt ttagcgatgc gatgcggcgt
    29461 ttccatcaaa ctgtcatcag ccaggtcgag attcagcagc tgcatgattt cggtcatatg
    29521 accagcaata aggcttttgc gcgtttcgtt atccatttca tgcacgggcg ggcgcagcgg
    29581 tgtttccagt cctcgcgcaa ctaacgcttc atgaaccagg gccgcttctt tactgagtga
    29641 tggcatttat gatttctcct gcaggtgtga cgcctccgcc ctgcgtgggg gcaaagttat
    29701 taagctgatt tacagcctga ttattgtgcg tgaggcggcg cacataatcc agtattcaca
    29761 gcgataatta ttgtaattgc cgctgccttt catcagcaga tgttaaaaca tcgttatgca
    29821 aatacggaag tgaaagttac tcacagcaca ttgaataaac ggtatgatga agaaattgca
    29881 aacaacacaa caaggagcca cgcatggaaa tgctcgaaga gcaccgctgt tttgaaggct
    29941 ggcagcaacg ctggcgacac gactccagta ccttaaactg cccgatgacg ttcagtatct
    30001 ttctcccacc ccctcgtgat cacaccccgc caccagtgct gtactggctt tccggattaa
    30061 cctgcaatga cgagaacttc accaccaagg cgggtgccca gcgcgtggcg gcagaactgg
    30121 ggattgtact ggtgatgcca gacaccagcc cgcgcggcga aaaggttgcc aacgacgatg
    30181 gctacgattt aggccagggc gcaggctttt atcttaatgc cacgcaaccg ccgtgggcga
    30241 cgcattaccg gatgtatgat tatctgcgcg atgaattacc ggcgctggtt cagtcgcaat
    30301 ttaatgtcag cgaccgctgc gccattagcg gtcactcaat gggtggtcac ggtgcgctga
    30361 ttatggcgct gaaaaatccg ggtaaataca ccagcgtttc ggcctttgcg ccaattgtga
    30421 atccgtgcag cgtcccgtgg ggaatcaaag cgtttagcac ctatttaggt gaggacaaaa
    30481 atgcatggct ggaatgggat agttgcgcgc tgatgtatgc cagtaacgcg caggatgcga
    30541 tcccgacgct tatcgatcag ggcgataatg atcagtttct tgccgaccag ttgcaacctg
    30601 cggtattggc agaagccgcg cgccagaaag cgtggccgat gacgctgcgt attcagccgg
    30661 gatatgatca cagttactac ttcatcgcct cttttataga ggatcacctg cgcttccatg
    30721 cgcagtattt actgaagtga aagtccgccc ggttcgccgg gcatcttctc atcagaagcg
    30781 ataatccact gccataaagt aacgacgtcc gtcttcgtta tagctgtagt cgtcacgact
    30841 gagatccttg tcgccaaggt tcagcacgcc tgcgcgcagt ttgacgtctt tagtcacctg
    30901 ccaggccgcg ccggtattcc agatggtata accgcccggt gttttagccg tcgcgctgtc
    30961 ggcgcgtttc tgcccggtat agtgaccaga aacatagaat gaccagtctt ccagcgccag
    31021 cggtttccag tccagcgtac cgttagcagt atggaacggc agatcggata gcggtttgtt
    31081 ttcgccgttg ctgacatcac gaccatcgtt gtaggtgtag ttgatcgaca gtttccattc
    31141 atcgttgaac ggaattttca gttcggtttc cacgccctga atacgagctt tgttaacgtt
    31201 gtagtagcta aataccggta tgcgccgtcc gttagcgccc gtctcaaaac caacaaagtt
    31261 ttggtagccc ggtgcagcgt tgacgtcaga cgtacggctg atgctgatac gatctttcac
    31321 atcgttacgg aaaacggtaa cgctggattc aaccccttcc agccagcctt cttcacccat
    31381 gtagtaaagc cccagctccc aactttcgct ggtttctggt ttcagatccg ggctacccac
    31441 aatcttacat gcgccacggc aggaattgct cgtccagtca gggctaagtt gcaacagaga
    31501 aggtgcttta aatgccgtcg cccagccccc tttcaccgtt acggtgtcgg tggcgttata
    31561 aaccaggtag gcacgcggac tccagtgttc accgtaggtt tcgtgatcgt ccatacgcac
    31621 gccggtcgtc agcgccagcg gctcgaagat ccgccattca tcttccacaa acagcgcgta
    31681 ctggctggca gacgttttgg agctggttcc cccggtcagg ttcaccgcat cgctaagttt
    31741 gtcgtgacgc cattcaccgc caaccgtgag aaactgatta atcgccgtca gcggcaacgt
    31801 gtatttgccg tcgaccgtat tgctttcgga agttatcggg ctgctgttgc cagggttttt
    31861 gttctcgact ttctcaccgt agtatttcag ttcgctggtg ccgtaatccc aacgcccatt
    31921 atggctgacg gagtagttct ggcgttccag gcggtttttg tccagcgagt cggaatcacg
    31981 atcctgacgg tcgaaaccgt atccggcagt aaaatcgtga ttttgattcg gtgtccaggc
    32041 aaattcgaca ttgccgtcgc ggctggagaa tccttcaata cgcggcgttt ctccggtatc
    32101 ggtggtcgtt gagttttgcg ggtcatcctt ttcacgtttt gccaggctgc cgtaagcttt
    32161 cattcccagc acaccatcaa ttaatggtcc actggtaaag aactgaccgt tataggtatc
    32221 accgcgatcg cgatgttcct gaatggtggt atcgacggta acggtacccg accatttctg
    32281 accgattttt ttggtgatga tattcactac accgccgagc gcatcggaac cgtacagcga
    32341 cgacatcggg ccacggacca cttcaatacg ttcgatggaa tcgaccggga tccagttcag
    32401 atcgaaatca ttgtggcgga agacggcatt gcgggagttc acgcgtttac cgtcgacgag
    32461 aatcagggta tagctgctgt ccagaccacg aatactaacg cccttacggt tatccccttc
    32521 gttcgtcagt tgtacgccag gcacttcttt gaggacatcc ttcagattct gtaccggttt
    32581 tcgctgcaga tcttcctggg taatgacgct gatactggcg ggtgcatctt taaggttttg
    32641 ttcaacggaa gatgcagtga caaccatcgt ttcgccatcg tcatcgaccg ctaacacagg
    32701 ccatacacaa gaaatagcgg acaaacacag cccgacccgt acgaaagggt tcaacctaaa
    32761 cattccatat ctccatgagg taactacgaa aataaaatgg gtatcgctca catcttcttt
    32821 acgtcccctt gcgtacggca gcatcgcggt ggacttattt gtatgcaggt gatcatccca
    32881 gaaagccgga ccagcctctg atttgtcagc cttttttgat tgtagcgtaa cgataatgca
    32941 aacgatagca attatcaatc caaatgttaa attttatatc cgcatgattt gtcagggaaa
    33001 tggaattgaa aaagccctct cggttgagag ggcttagcaa ggaagggagg aaacttattt
    33061 cttatcgttc tgcgggaact tcatttcgct gtagcgtacg aagtgagttc ctttaatcag
    33121 cttgtagccg aaccaaataa tcaggaacag cgggatacca atatacgttg ccgctacgcc
    33181 gccccagtca atggtatctt tcaggaacgc ttcgtagttc tggcccaaag tgataatcag
    33241 acacagaatg aatgcgaaga tcggccccag tgggaagaaa cctgaacggt acggcagatc
    33301 gttaatgtcg tgtccctgca atacgtaacc gcgacggaag cgatagtggc taatggcaat
    33361 ccccagccag gcgataaaac ccgtcatccc ggaggtgttc agcagccaca ggtataccgt
    33421 ctggttgcca aacatggagg tcaggaagca caaaccggca atcaccgtcg tcgcatacag
    33481 cgcattacgc ggcacgccac cacgcgacaa tttagcgaaa atgcgcggcg ctttaccgtc
    33541 acacgccagg gtgtacagca tacgagtaga cgcatacata ccggagttac ctgctgacag
    33601 caccgccgtc agaataactg cgttcatcac cgccgccgca gagagcagac ccgcgtgctg
    33661 gaacaccaag gtgaacggac tgacgctgat gtctttaaca tcgttacgca gcaggctcgg
    33721 atcggtatac ggaataatca ggctgataat caggatcgcg aacacataga acaacaggat
    33781 tcgccagaat acctgacgta ccgcgcgtgg aatgtttttc gccggatctt cggactcgcc
    33841 tgcagcaata ccgatcagct cggttccctg gaaagagaag ccgacaatca tagctacgcc
    33901 gatcatcgcc gcaaaaccac cagcaaacgg cgcttcgccg attgtccagt tgctccagcc
    33961 cgcaggctgc gcgcctttga agataccgat aatcatcagc acgccaacga tgataaagac
    34021 aataactgtc gtgactttga tcagtgaaaa ccagtattcc gcttcaccaa agccacgaac
    34081 tgagatgtag ttcagcagga agataacgcc gaggaacaac gcactccaga tccagcccgg
    34141 tgtatccggg aaccaccagc tcatgaccag ctgtgctgca accaagtcaa cggcaatagt
    34201 caccgcccag ttgtaccagt agttccagcc cagcgcgaag ccaaagcctt cttcaacata
    34261 gttctgaccg taagtggcaa acgaaccgga aaccggcata tatgcagcca attcaccgag
    34321 actggtcatc aggaagtaaa ccatcaggcc aatcagcata tacgagagca atgccccgcc
    34381 cggacctgcc tgagaaatcg ttgcgccaga ggcaacaaaa agacctgtac cgatggaacc
    34441 gccaatggca atcatcgtca ggtgacgcgc ctttaattca cggcgtaagc ccggcgcttc
    34501 tgtagtttta gtttcggaac ccatacaaaa atgctatcca tcttaaaaat gaggcgcgat
    34561 tgtagcagac gattggcaat ccttccggca gaaatacacg gttataagac accttcatga
    34621 tcgcccaggg attataagta aagcagccaa tctcttttct ggcgaatgca tgctgagggc
    34681 gctgcgtcac gaaatcgaca cagcaccagc atgttcttgt acagcacctt aacgcggtac
    34741 attcgcggga tcgcaatagt ccagaaagcg ccgtagcgcg ttggaaaggt gtttttgccg
    34801 atgatgtata cgccacaacg tacgcatcag gcgcgggaga gggaccgcaa cttcacttaa
    34861 tgtgcctgcc tgcaattgat cttcaatcac acgtcgcgac aggcaactaa ttcccaaccc
    34921 atgacgcacc gcatgtttga ttgcctcgga gttacctaat tccatcgcca tctcaaactt
    34981 cggtaaatgt gacagcaaca gataatcgac aatctcccgc gtgccggaac cgcgttcacg
    35041 caggatccac ggcgcagcgg ccagctgttc taaggtgacc ggaccacgag ccaacggcga
    35101 agtcggcgcg gcgaaaacca ccagctcgtc ttccagccac ggttcagaaa tgatttcagt
    35161 gctgtggcac ggtccttcaa taaagccaat atcaacgcgg aaatccagca ccgcttgcat
    35221 cacgtcctgg ctgttcccaa cgctaagttc aatcggcaac tgcggataat catggcgata
    35281 acgggcgata actgcaggca gaatgtagtt accgatggta ctactggcat agatacgaat
    35341 cgcgccgttg tcttcgcgaa acagttgttc gatttcaacc gcctgttcaa gcaatgccaa
    35401 cgcacgcgga tagagcagcc gcccgtgttc attaacaacc agtcttttcc ccacgcgatc
    35461 aaacagttgc acgccaagct gcccttccag gtcggtcaag gctgcgctca ctgctgattg
    35521 cgacaacgcc agcatcaccg acgcctgggt ggttgatcca cttttcaata cttctgcaaa
    35581 aacttccaac tgccggaggg tgatgtgcat agtcgcttac cacttataaa gattaattat
    35641 aaatatataa tcaattttat ttttaaacca gttagtcgta accttatgcc cggttaaagg
    35701 agagggttat gacgaatatc accttacaga aacaacatcg tacactgtgg cattttattc
    35761 cggggttagc cctgagtgca gttatcaccg gggtcgccct gtggggtggt tccattcccg
    35821 cggttgcggg tgccgggttt agtgccctca ccctcgcaat cttgttgggg atggttttag
    35881 gcaacaccat ctatccgcac atctggaaaa gctgtgacgg tggtgtgctg tttgccaagc
    35941 aatatttatt acgtctgggt atcattcttt atggcttccg tctgacgttc tcgcaaattg
    36001 ccgatgtcgg tatcagtggg atcatcattg acgtgttgac gctgtccagt accttcctgc
    36061 tggcttgctt cctggggcag aaagtgtttg gtctggataa gcacaccagc tggttgatcg
    36121 gtgccggtag cagtatctgt ggtgctgccg cggtactggc gactgagtcg gtagtgaaag
    36181 cggaagccag taaagtaacc gtggctgttg caaccgttgt tatcttcggg accgtcgcga
    36241 ttttcctcta cccggcgata tatccgctga tgtctcaatg gtttagtccg gaaactttcg
    36301 gtatctacat tggttctact gtgcacgaag tggcgcaggt ggtggcggca ggtcatgcca
    36361 tcagcccgga tgcggaaaac gcagcagtta tttccaaaat gctgcgcgtg atgatgctgg
    36421 ctccgttcct catcctgctg gcggcgcgtg ttaaacagct gtctggggcg aacagcggcg
    36481 agaaaagcaa aatcactatt ccgtggtttg ccatcttgtt catcgtcgtt gccatcttta
    36541 actcgttcca cctgttaccg cagagcgtgg tgaacatgct ggtaacgctg gataccttcc
    36601 tgctggcaat ggcgatggcg gcgctgggtc tgaccactca cgtcagcgcg ctgaaaaaag
    36661 ctggggcgaa acctctgctg atggcactgg tactgtttgc ctggctgatt gttggtggtg
    36721 gtgcgattaa ctatgtgatt caaagcgtca tcgcataaac cactacatct tgctcctgtt
    36781 aacccgctat cattaccgtt ttcctccagc gggtttaaca ggagtcctcg catgaaatac
    36841 attggagcgc acgttagtgc tgctggcggt ctggcaaatg ccgcaattcg cgccgccgaa
    36901 atcgacgcaa ccgcgtttgc cttgttcacc aaaaaccaac gtcagtggcg tgccgcaccg
    36961 ctcacgacgc aaaccatcga tgaattcaaa gccgcctgtg aaaaatatca ctacacatcg
    37021 gcgcaaattc ttccccacga cagttatctg attaacctcg gacatccggt cactgaagct
    37081 ctggaaaaat cgcgcgatgc ctttatagat gaaatgcagc gttgcgaaca actggggctt
    37141 tctttgctca acttccaccc tggcagccat ctgatgcaga tttcagaaga ggattgcctt
    37201 gcgcgtattg ccgaatccat caacattgcg ctggataaaa ctcaaggtgt aacagcggtg
    37261 attgaaaaca ccgccggtca gggcagtaac ttagggttta aattcgaaca tctcgcggcg
    37321 attatcgacg gcgtggaaga taaatcccgc gtcggcgtct gcattgatac ctgccatgct
    37381 ttcgctgccg ggtatgattt gcgtactcca gccgaatgcg aaaaaacgtt cgctgatttt
    37441 gcccgtattg tcggctttaa gtatctgcgc gggatgcacc ttaacgatgc gaaaagtacc
    37501 tttggcagcc gcgttgaccg ccatcatagc ctcggtgaag gcaatatcgg tcatgatgcg
    37561 ttccgctgga tcatgcaaga cgaccgtttc gacggcattc cgctgatcct tgaaaccatc
    37621 aacccggata tctgggcaga agagatcgcc tggctgaaag cgcaacaaac tgaaaaagcg
    37681 gtggcctgat cgatgaacga cagggaaaag cagattctta aaattttacg gcgtaacccg
    37741 ctgattcaac agaatgaaat cgccgatatt ctgcaaatca gccgctcacg tgttgccgct
    37801 catattatgg atttgatgcg caagggtttg attaaaggca aaggatatat ccttaccgag
    37861 caggattact gtgtggttgt gggtgcaatc aatatggata ttcgcggaat ggcggatatc
    37921 cgttacccgc aagcagcctc tcatccaggt tctgttcact gttccgcagg cggcgtcgga
    37981 cgtaatatcg cgcataacct ggctctgctt ggtcgggatg tgcatttaat atcagctatt
    38041 ggcaacgatt tttacggcga gacattactg gaagaaacgc gccgcgcggg cgtgaacgtt
    38101 tcaaactgca ttcgtctgca tggacacagc actgcaactt atctcgctat cgctaataag
    38161 caggaagaaa ctatcctggc aatcaatgac acacatattc tgcaacagct aacgcctcaa
    38221 ctgctcaata cttcacggga cttgattcgc catgctggtg tagtgctggc tgattgtaat
    38281 ctcacacctg aagccctgga gtgggccttt accattgctg atgaaattcc gatgtttgtc
    38341 gataccgttt ccgaatttaa agcgaacaaa gtaaaaaact ggtacagccg gatccacact
    38401 ctgaaaccaa cacaaaatga actggaaatt ttgtgggggc aaccgattaa agacgacaac
    38461 gatcgcatca gagctgtaaa ttcgctgcat caacaaggtg ttaaacgcat ttttgtttat
    38521 ctgaaagatg aatctgtatt ttgcagcgat aaggatggag aacaattttt gctgaccgcg
    38581 ccagcgcata cgacagtaga cagttttggt gctgacgatg gttttatggc gggcctggta
    38641 tatagctttc ttgaaggata cagtttccgc gacagcgccc gttttgcggt agcctgcgcg
    38701 gcaatttcgc gcgccagcgg cagcttaaac aaccctaccc tgtctgccga taacgcgctt
    38761 tcattagtgc caatggtgta acaatgttgc cggattcgac gcttgaccgc atcttatccg
    38821 gctcataaat tgcgcactac gctaaaccaa taaagaatcc tgcaatagta gcactcatca
    38881 ggttagaaag tgtcgccgcc gccagcgcgc gtaaaccaag ttgggcgatt tccggcgcac
    38941 ggtgtggcgc aaccgcagaa aacgccccca ccaccacccc gatagaacca aagttagcga
    39001 aaccgcacaa cgcgaaagaa ataatcgcca cggttttagc atccagagtg ccacccgttt
    39061 gcagatatgg cgagaaattg agataagcga caaattcatt gatcgccagc ttctgcccaa
    39121 tcaaactccc ggcaagattt gcatcactcc agtcaacccc catcacccac gccaatgggg
    39181 ccaacaggta acctaaaatc gactccagcg aagcatgttc aaaaccaaac cagccgccaa
    39241 caccgccgat aataccgtta atcaacgcaa ttattgcaac aaatgccata acaactgtcg
    39301 ctacaccggc ggcgattttc agcccggtca ttgcccctgt cgcagcggct tcaataatgc
    39361 tttttggcgg tgtttcggtg aaagagaggt tattaaaaga aacctgcgaa gattccgtag
    39421 ccgggcttaa caggcgggca aacaagatcc cgccagggat cgccattaac gatgccgcca
    39481 gcaaatattc cacaggtacg cccagtgcgg cgtaaccaat cattgtcgaa ccagcaatcg
    39541 aggccatgcc actacaaatc gctgtaaata attcattgcg attcagacga tcgataaagg
    39601 gtttgacgat cgccggaatt tcgttttgcc cgaggaaaat ggtggtgacc gcgacgaatg
    39661 actcgatctt gctgatattt aaagctttct ggaagatacc gccgagaatg cgaattaaga
    39721 tccccatcac accgatgtag tagagaatac tcaccagtgc agtgacgaag ataattgccg
    39781 gtaataccct gaaaccaaag ataaatcctg cgccatcaaa taaggtatcc attttcggtc
    39841 cgaccagaga accgaagata aatgcgctac ctgcgtcgct gtacgccatc actttgtgga
    39901 cgccaaaagc gactttttct gcgacccaac gccctggcgg taaccaaagc ataatcccgc
    39961 caatcacgac ctgtaacact aacgccgcgc caacggtacg caggctgatc ttcttcttgt
    40021 ttactgacag taaaaacgca attgccagca gtaccaccat tcccagaaca cttctcatga
    40081 catccatagt gattatctct tcatgtcatt ttgggcgacg gctttctgcc catcagaaat
    40141 agccgggcct gcatcttgcg atactgtccc ggcttgttat caacacttaa tgggttttta
    40201 tgtagccgcg tacgcactct tcgactaatc cccagaacca gtcggtatca atagtgatgc
    40261 cgactttagt attggcgggc ttgccaagaa cgctcagctc gtcgcagacg gtacgcccat
    40321 agcaagggcc actgttgacg tccacttcga cgtacatctc ctgggtttta atgccatcag
    40381 ggttaatcag ataaccgatg caggtggcgt cgtgcaccgg gccgccagcc aggccgtagt
    40441 tttcgaactg cgttttgaga gtgaagttca tgatgtcgct gaacatctct ccggcagggc
    40501 cgcctgccct ttccattcgt gcaattacgt ccggggtgca aacggtctgg ttggtaagat
    40561 cgaggcccat catcactaat ggaacgccgg aggtgaacac cacgcgtgcg gcttccgggt
    40621 cggcaaagat attgaactcg gcagatgggg tgaagttgcc tgtaccgtaa gcgccgccca
    40681 ttagcacaat ttcacggatt ttgggcagga ttgcgggttg catacgcatt gccaccgcga
    40741 tatttgaaag cggaccaacc ggcaccagag tgatatcgcc atcgcttgcc ataagggtat
    40801 caatgatgta tttcaccgcg tgggtacttt ctgcctggcg ggtcagcggc tcgaataccg
    40861 ggccatccag tccggtttca ccgtgaatat tatcggcaac gatttgttga cgcataatgg
    40921 gttgcggcat ccccgcataa accggaacat taatctccag tttctggcaa acattcaggc
    40981 cattaattaa tgttttatca agcgtctgat tacccgcaac aatggtgata cctaataaat
    41041 caatcgccgg atgtttcgcc gccatcatta tagcaatagc atcatcatga cccggatcac
    41101 aatccagaat aatttttctc ttttccattg tttatttcct ctgtttccag ttgcgttatt
    41161 ttttctacag caacgaaatt tcgatcgggc cgatgattga atcttaacaa cagcgtacga
    41221 atgctaaata tgagaaatct catagcggat aaacatcgtg aaagaaatcc acaataatga
    41281 tcttaagcag caattgatga gtgaatccgc gtttaaggat tgctttttaa cggatgtttc
    41341 agccgatacg cggctgtttc attttttagc gcgtgactac attgtgcagg aagggcaaca
    41401 gccgtcctgg ctgttttacc tgacgcgagg ccgcgccagg ctttacgcca cgctagctaa
    41461 tggtcgcgtg tcgctgatcg atttctttgc cgccccctgt tttattggcg agattgagtt
    41521 aatcgacaaa gaccatgaac cgcgtgcggt gcaggctatt gaagagtgtt ggtgccttgc
    41581 gctccctatg aaacattacc gcccgctgtt attaaacgac acgctatttt tacgaaaact
    41641 ctgcgtcacc ttaagtcata aaaattatcg taatattgtt tctttaactc agaatcaatc
    41701 atttccgtta gtgaatcgcc tggcagcatt tatattactc tcgcaggaag gtgatcttta
    41761 tcacgaaaag catacgcaag cggcagagta tttaggcgtt tcttatcgac atcttttata
    41821 tgttctcgcg caattcattc acgacggttt attaattaaa agcaagaaag ggtatctaat
    41881 taaaaacaga aagcagttgt caggactggc gctggagatg gacccggaga ataaattctc
    41941 cgggatgatg cagtaaaaat tattggcaat agcacgattg ccggatgcaa cgcttaataa
    42001 ggcgtacatc cggcaatgaa actcaagcta aaccaataaa gaacccggca atggtcgcgc
    42061 tcatcaggtt ggaaagcgtc gccgccgcca gcgcccgtaa accaagctgg gcgatttccg
    42121 gcgcgcgttt tggcgaaata gccgaaaacg ctccaacgac aacaccgata gaaccaaagt
    42181 ttgcaaaccc acagagggcg aacgagataa tggcaatggt cttgacatcc agcgtgccgc
    42241 tcgtttgcag atagggcgag aaattgagat aagcaacaaa ctcattaatc gccagtttct
    42301 gcccaatcaa acttccggca agatttgcat cactccagtc cacccccata atccatgcca
    42361 acggggccag cacatagccg aaaatgcttt ccagagagac attggcaaaa ccaaaccagc
    42421 cgccaactcc accaataatg ccgttgatca gcgcaatgat cgctacaaat gccattacca
    42481 ccgtcgccac accggcggcg attttcagcc cggtcattgc accgttcgcc gcagcttcaa
    42541 taatgctttt tggcggtgtt tcgctaaacg acagattttc aaacgtaact tgcgaaggtt
    42601 ccgtcgccgg actaagaata cgtgcaaaca ggataccgcc aggaatagcc atcagcgaag
    42661 cggctaacaa atagtcaatc ggcacgccca ttccggcata gccaatcatc atcgaacccg
    42721 caatagacgc catgccgcta caaatagccg taaatagctc gttgcgattc atgcgatcaa
    42781 taaaaggttt aacgatcgcc gggatctcat tttgcccgag gaaaatagtg gtgactgaga
    42841 caaaagattc gattttgctg atgttgaggg ctttctgaaa aatactgcca agaatacgaa
    42901 tcagcagccc cattacgcca atgtagtaca gcaagctgat aagcgcggta acgaaaataa
    42961 tcgccgggag tacgcgaaag gcgaagataa agcccgcacc gtcaaacaga acatccattt
    43021 tgggaccgac cagcgaacca aaaataaagg cacttcctgc gtcactgtag gacatcactt
    43081 tatggacgcc taatgccgcc tgctctactg cccattttcc cgggggaaaa tagagcatga
    43141 tgccaccaat ggcaatttgc agcagcagcg cagcgccaac cgtgcgcaag ctgatgctct
    43201 ttttatttac cgacaacaca aaggcaattg ccagcaatac caccatcccc gcaacacttc
    43261 tcattatatc cataatgatt ttcccttcat gccagtaaac ccggcgtcag cgccaggttt
    43321 tggtatgttt gatgagtacg ggcgacggct ttctgcccgc cagaaattaa cccacgagac
    43381 gctgatattc tttggcaatt tcgctcgcca gaatggcgtt attaaagacc agttgaatat
    43441 tggatttcag gctatcaccg ccggttagct ccgcaacacg cgccagcagg aacggtgtac
    43501 tctctttccc aataacaccc tgttcttcgg cttcagcaac ggcttgatca attgctgcat
    43561 tgatggtttc ttctggcatc gcaaactgtt ccgggatcgg gttcgctacc accaggccgc
    43621 cgttcaaacc ggtttgccac ttcactgcca tcgcgcgagc aatttcgcgg gcactgtcaa
    43681 gacgaatgct gacttcaaat gggctggttc ggcagaaaaa cgcaggcagc gcttttgtct
    43741 gataaccaat taacggtaca ccgaaggttt ctaaatattc tgttgttaat cctaaatcga
    43801 gaatagattt tgcccccgca caaacaacag tgacattggt atttgccagt tcctgtaaat
    43861 cggcagaaat atcgaaggta tgttccgctc cgcgatgcac accaccaata cctccggttg
    43921 caaaaacttt aatcccagcc agtgctgcaa taatcatggt tgatgcaacg gtagtcgcgc
    43981 catttttccc tgcagcaacg acaaaaggta aatcacgacg actaactttc gtcacgttat
    44041 gtccttcacg gcccagcaat tcgatttctt ctttacttaa gcccactttc atcacaccgc
    44101 caataatggc gatagtggct ggtactgcgc cctgtttacg aattgtttct tctacttcaa
    44161 ttgcggtttg ggcattttgt gggaatggca ttccgtgaga aattatggtc gattccagcg
    44221 caacaaccgg ttttttattt tttaaagcat cctgtacttc agcggatatt tgtaataatt
    44281 caggggacag agttaattca gacattctgt gttctccact aacgatctaa cattggcaat
    44341 tgataattca gggttgttgg tatattcaca ggcaagtgcc attgacgaac atccctgcgc
    44401 aaatcgaaca gaatcgataa acggcatacc gtctacccaa caggaggcca gccccgccat
    44461 catggcgtcg cctgccccag taacattgat gacattcgtt ttaatagggg ccgaccaccc
    44521 actttcaccg ttgatatcac tgtagtaaac gccatcccct cccatgctca gcaccaagcg
    44581 gttcaggccg tgttgatgaa accaggcggc gacctttgcg acatcttcac gtccggaaag
    44641 tgcaattccg ctcaacgttt cggcttcaag acggttaggt tttaacgtat ggattttgct
    44701 cagatgatcg cggattttga cgcatttcca tgccgatacc ggatcaataa atacaggcgt
    44761 ttccccgcta ttttccagaa cccagaccag cgcctcttca ctaaggttgc aatccgcgac
    44821 aataaccttt gcaccgcgaa taaactcctg atgctgggca agaaattctg cagaaataca
    44881 gtcactaata ctcatatcat ttatcgcgac aagcatttct cctgtattat cgagcagtga
    44941 taaatagctg gatgtattct cacccggaac aattaagcat ttatcgacat aaacaccgga
    45001 ttgattagtt tgcgccagta atgattgccc ataaaaatca ccacctactg cacttaaaag
    45061 ccaggcattt ttccccagta acgccagatt atgagcgata ttccttccca cgccacctgg
    45121 ggtaaattta atttttccgg gattcgaatc cgcataattc aatggatgat gtgaataccc
    45181 agccacatca atattcgccg aaccaataat tactatgtag tccttttcgc gcatagcacg
    45241 ccccctggca ataaattgcc aacgaagaaa ttgggtaaaa tagccattac agtcaataag
    45301 actattagag cacgtgttca gatttaaaca catgttcata ataagcatat ttaacgtaat
    45361 gtaaatcgat cagaaaaaac gaaatcggcc agatatgaag gcggtcacaa agtttgtcta
    45421 tttatgcagc aacaaaagag aaagttgtga tgtgtaacac acaatatggc aggcaagaaa
    45481 aagggcagag atgtttctgc cctccagatt gttgacaacg cgcgctttgt tcatcctgga
    45541 tgtggcgtaa acgccttatc cgacctacaa aagcgcaaaa aattcaatat attgcagggt
    45601 tcatgtaggt ctgataagca tagcgcatca ggcaattttg cgtttgtcat catcggggca
    45661 gagatgtctc atccccgata atgaagataa attatgcgtt tttttctact atttgtgttt
    45721 ccgggcgttt caggaaggca taggccaaac ccgccaccag cgtaccggca ataattgcta
    45781 ccaggtaacc caataccggc gtaatagcgc cagggatcag cagaacaaac agaccaccgt
    45841 gtggtgccat cagtttcgca ccaatcgcca ttgagattgc gccagtcagc gccccaccaa
    45901 cgatacagca cggtagcaca cgcatcggat cacgagcagc aaacggaatt gcaccttccg
    45961 aaataaagca cagaccaagt accagagcgg ctttgccacc ttcctgctgc gctttgtcga
    46021 atttgcgacg cgccaccatt gttgccagac ccattgccag cggtggcacc atacctgccg
    46081 ccataatcgc cgccatcggg ccataggttt gagtactcag cagacccaca ccgaatgcgt
    46141 acgctgcttt gtttaccgga ccgcccatgt cagtacacat catgccaccg aggatcgccc
    46201 ccagcagaac cgcattcgca gtccccatgg tctgcagcca gtgagtcaac ccatcgagaa
    46261 tgccagcaac cggtttaccg attaggtaga tcatcgccag accgaccacc agactggaaa
    46321 ttagcggaat gatcaggatc ggtttcagcg cctccatact ctgtggcagt ttcaattgcg
    46381 tactgattaa cttcgcaatg taaccagcca ggaagccagc aattataccg ccaataaagc
    46441 cagaaccggt gctgaccgcc agcataccgc caatcagacc cggtgtgagg cccggacgat
    46501 cggcaatgga gaaggcaata taacctgcca gtaccggcac catcagcgca aaggctgaac
    46561 caccaccaat ctgcatcagc gccgctgcca acgtacccgg ctctttaaac gcttcgatac
    46621 caaaagcaaa agaaagcgcg atacacagac cacctgcaac aaccatcggc agcatgtaag
    46681 agacgcccgt cagcaagtga cggtaagcgc ctgcactctc tttcttacct tcagtggtcg
    46741 ctgtttgagc tttgcccgct ggttcatacg gcgttgcttc agcaaccgct ttatccagtt
    46801 cctgcgcagt tttcttcagc gccagaccgg tagaggtgcg atacatcggt ttaccagcaa
    46861 atttcgccag atccacttcg atatctgccg ccacaatcac cagatccgct gctgcgactt
    46921 cttcaggggt gattgcattg cccgcgccaa cagaaccacg ggtttcaact ttcacccacc
    46981 agccacgttt tttcgcttcg gtttcaatgg cttcagccgc cataaaggtg tgtgctacgc
    47041 cagtcgggca agcagtcacc gcaactacgc gtttcggacc gctggcagca accggtgctg
    47101 tcgcagcgac cggcgcagtg taaggtttcg catggccttt ggcttcactc aggaacagct
    47161 cagggtgcgc aactgcccgg gaaatatcac ccagccagac atttttaccg ttcagcgcgc
    47221 tgtcattcgg gatggaatca ccgagaacaa tcgccatttc tgcgtcgttc ggattgtcga
    47281 tgatttccag ttttgctttt cgcgccgccg cgcccagcag ggtcttcgcc atataggcgc
    47341 gtgcctgacc gagattagcg tcaataatca gcagcgtttt cattatgcct ctcctgctgt
    47401 cagttaaaag gttgtaagtc gacgcgcgcc atcattgcgg ccaactgcgg acgatcggta
    47461 atacccacat tgctttgact taccgccagg gctgcaacag ctgtcgccag acgcagtgtg
    47521 tgttcactgg attcacgcat cagcaagcca taaatcaggc caccaaccat agaatcccct
    47581 gcgccaacgg tgcttacgac atcgactgac ggtggtttgg cgatccattc gccggaggca
    47641 ttaacccaaa gcgcgccttc ggcacccagt gaaataacaa catgcgcaat accttgttca
    47701 cgtagcgcat gtgcagcttc aatcacatct ttcatttcag gcagtttacg gcctgcccag
    47761 atttccaact cgcggcggtt aggtttcacc agccacggtg ccgctttcaa acctgctact
    47821 aacgcttcac ggctactatc aaagataatg caaggacact gactacgcag gcgagtcatc
    47881 cagtcggtga acgcttccgg gctgacgcct gacggtaagc ttccgctgac acagaccata
    47941 tcgaactgac cgagccagct cagagaatca gtcacaaagc gttcccagtc ggcgagggtg
    48001 acttcaaaac ccgagaagtt gaagtcggtc acttcgccgt ctttttccgt cagcttaacg
    48061 ttaattcggg tgcgcccctg tacaacctgg aaacggttgg caatgcccag ctcgctgaac
    48121 agttgctgaa aaccatcctg attgtcttta cccaggaagc cgccaacggt gacatcaatt
    48181 cccaggtctt ttaatacttt ggccacgttg atgcctttac ccgccgcatg cagaccggtg
    48241 gttttcacca ggttcacttc gccgcgttca atttccgggc agaaaccaac aaggtcataa
    48301 gccggattaa gggtgatcgt agcaacacgt ctgctcatta tgcgccctcc ccaagaccag
    48361 cagcgatagc gtcgccaatg gctttcagcg cctgttcagc atctgcaccc tgggcggtaa
    48421 agcgtaggcg atgacctttc ttaacgccaa gtgccacaac tttcatcaga ctacgtccgt
    48481 ttgccggttt gccggtgcca tcaaggtttg tcacggtaat atcactgtta aattgtttaa
    48541 tggtattgac cagcatggta cctggacgag catgcaggcc gtgttcattg cgcaccacaa
    48601 actccgcgct taacacgtcg tcggtcggcg catcatcgct ggtcagcagc gccagcaacg
    48661 ttgccgcatc cgctttcagc aagcggtcag ctttattgtc gagcaataaa tcagcgagac
    48721 gcttaagaac cgcgatgggc tgatcgtcat tcatcgccac actcaccagc atggctgccg
    48781 tttcgccgtc cacatcaaaa gcatttgccg cacggcttac cgcaatcgcg ctacgcagat
    48841 tgccttcggc gctatcgctc agccagatac cctgtccgag attcagcggt tgttcattga
    48901 tggctttggt gacgaaagtg gcgtcaactg cccccacctc tttcagacgc gcagcgttca
    48961 gcgcctgaag agtcagcaga tcgctggcga cgatatccag cgtcagcatt tcgttgtcga
    49021 gcttcagctg ctcactctgc ttttcgccca tcagtaatgc gcgaagttct tctgctgttg
    49081 ttgctgactt cagttgttca gcaacggaat catcgctcag tacgtgggtc agctggcgta
    49141 gcaggcccag atgttcatcc gagctggcag caataccgat tgccacgtac gctacctgac
    49201 cgtcacccca ggtgacgcct tccgggaact gaaatacctg aacgccggtt ttcagcacct
    49261 gatcgcgggt gtcggtagtg ccgtgtggaa tagcaatacc attgccgagg aacgttgagg
    49321 tttgctgttc gcgcgccagc atgccattga cgtagccttc tgctacatta ccggcctgca
    49381 ccagcgccgc agcgacctgg cgaatcgcct cttctttgtc tccggccttt tcgcccggat
    49441 ggatgtcctg tacggataac tggaacatag ttctcctctc ttgctgaatt gaaacgattc
    49501 agcctctatg agaaaaaaag cgccaacctg gcttagggtt aaagacaaga tcgcgctgaa
    49561 acgtttcaag aaagcataat acttctgttt cagcacgcaa ggaaagccgt aaagttatga
    49621 gcaaaagttt gatgtgctgc acaatttttt gtcatttttc ctaattgctg atgggaaaat
    49681 ccgttatcag caatttcatt tcagcagcgt cagcagccaa tcagcaacag tgaaatggca
    49741 ttttgattat tcgtggcgct tttgatttcg ctgtctgttt attttctgac aagcagcgta
    49801 aactctgcgt cttcctcttc cagtgatcga cctgcatgca taactccccc gcagtctcca
    49861 gcgcgaaatc gtttgacctg acctcgacgg cgtttttaat cgttgccttt ctcaccggta
    49921 ttgcgggtgc tctgcaaacc ccgacactca gtattttcct taccgatgaa gtacatgccc
    49981 gtccggcgat ggtgggattc ttctttaccg gcagcgctgt cattgggatt ctggtaagtc
    50041 agtttctcgc tgggcgctct gataagcgcg gcgatcgcaa atcgctgatt gtcttttgct
    50101 gcctgttagg cgtgctggcc tgcacccttt ttgcctggaa tcgcaactac tttgttttgc
    50161 tattcgttgg cgtctttctt agcagctttg gctcgaccgc taacccgcaa atgtttgccc
    50221 ttgcccgtga acatgccgac aaaaccggac gtgaggcggt gatgttcagc tcttttttac
    50281 gcgctcaggt ttcactggca tgggtcattg gcccaccgct ggcttatgcg ttagcgatgg
    50341 gtttcagctt tacggtaatg tatctgagcg cagcggtagc gtttattgtt tgcggcgtga
    50401 tggtgtggct gtttttaccg tcgatgcaaa aagagcttcc gctggcgacc ggcacggttg
    50461 aagcgccgcg ccgtaaccgt cgcgatacgc tactgctgtt tgtcatttgt acattgatgt
    50521 ggggctcgaa cagcctgtac atcatcaaca tgccgctatt tattatcaac gaactgcatc
    50581 ttcccgagaa actggccgat gtgagatggg gaccgccgcc gggctggaaa tcccgacgat
    50641 gttgattgcc ggatatttcg ccaaacgtct gggtaagcgt ttcttaatgc gcgttgctgc
    50701 cgtgggtggc gtctgttttt acgcaggaat gctgatggcg cattcacctg tcattctgtt
    50761 gggcttgcag ctgctaaatg ctatttttat tggcattctg ggcggcatcg ggatgctcta
    50821 ttttcaggat ctgatgcccg gtcaggcggg ttcagccacc acgctctata ccaacacttc
    50881 gcgcgtgggc tggatcatcg caggatcagt ggcgggcatc gtcgccgaga tctggaatta
    50941 tcacgctgtg ttctggtttg cgatggtgat gattatcgcc actctgtttt gcttactgcg
    51001 gattaaagat gtttaaggcg cggtcagcat ttccagatcg ataagccagg tcatggcctg
    51061 ctgacgcgag ttaccgcaca tttcagcgct ggcttgtaag cctgcacaga ctttcggtcg
    51121 cagtggcgag gtgaaaattt tacagcgttg ctgttcatca agctgaatgc agggcgtatt
    51181 ggcgggcttg ccatctggca taccgggaat agggctggaa attgaagggg cagtgcaaca
    51241 cgccccgcaa cccggacggc attccatacg ttgttctctt aaggttaacc accggattga
    51301 tgagcgcgca cagtaccacc ttttttgcac cagcaaaagt gcgaatacca cttgcccgaa
    51361 aggcccgtcg cgagtacttt gtcgcgatat ttttgacatt ttcgactaca ggaatttttc
    51421 gatgccaaga gcgaacgaaa ttaaaaaagg tatggtactg aattacaacg gcaaactgct
    51481 gctggttaag gatattgata ttcagtcgcc cactgcccgc ggtgccgcta cgctgtacaa
    51541 aatgcgtttt tctgatgtcc gtaccgggct gaaagtagaa gagcgtttca aaggtgatga
    51601 tatcgttgac accgtgacgc tgacccgccg ttacgttgac ttctcctatg tcgatggcaa
    51661 cgaatatgtc tttatggata aagaagacta taccccgtat accttcacca aagatcagat
    51721 tgaagaagag ttgctgttta tgccagaggg cggcatgccg gacatgcagg tgctgacctg
    51781 ggacggtcaa ctgctggcgc ttgagctgcc gcagaccgtt gatctggaaa tcgttgaaac
    51841 cgcaccaggc atcaaagggg catctgccag cgcccgtaac aaaccggcga cattgagcac
    51901 tggtctggtg attcaggtac cagaatactt aagcccgggc gaaaaaattc gtatccatat
    51961 cgaagaacgc cgttatatgg ggcgtgctga ctaacttcag ccgcatgcag aaaagggata
    52021 gctcaggctg tccctttttt aatttattat accaatcttc tattttgcgc ttcctgaaca
    52081 ccttacgccc tcaattttca ctcgttgatt gatcgccctc acacttcatc gcattaacaa
    52141 tccagaccaa tttcaattgc tgtcatataa ctttacactg tcattgttaa tttatcgtta
    52201 ctaagacgtg actcctatga agacaattgc ctccgttacg ctcccgcatc atgtacacgc
    52261 tccacgctac gatcgccagc agttgcaatc acgtatcgtt cattttggct ttggcgcctt
    52321 tcaccgcgct catcaggcgt tactgaccga tcgtgtgctg aatgcccagg gcggcgactg
    52381 ggggatctgt gaaatcagct tgttcagcgg tgatcaactg atgagccagc tccgcgcaca
    52441 gaaccattta tataccgtgc tggagaaagg tgcggacggc aatcaggtga taattgtcgg
    52501 tgccgttcac gaatgcctta atgcaaaact ggattcctta gcggcaatta ttgagaaatt
    52561 ttgcgagcca caggtggcaa ttgtttccct gacgattacc gaaaaaggct attgtattga
    52621 cccggccacc ggtgcactcg acaccagtaa tccgcggatt attcacgatc tacaaacccc
    52681 tgaagaacct cactccgcac cgggtattct cgtcgaagca ctgaaacgcc gccgtgagcg
    52741 cggccttaca ccgtttaccg tgctctcctg cgacaatatt cccgacaatg gccatgtggt
    52801 gaaaaacgcg gtgctgggaa tggcagaaaa atgttcgcca gaactcgccg ggtggataaa
    52861 agaacacgtc agttttccgg gaaccatggt cgaccgcatt gttccggctg caaccgacga
    52921 atcactggca gaaatcagcc agcatctggg ggtaaatgat ccctgcgcga ttagctgcga
    52981 accgtttatc cagtgggtgg ttgaagataa cttcatcgct gggcgtcctg cctgggaagt
    53041 cgcaggtgta caaatggtga atgatgtcct gccatgggaa gagatgaaac tgcgtatgct
    53101 taatggcagc cactcttttc tcgcttatct gggttacctc tcaggattcg tccatatcag
    53161 tgattgcatg caggatcgcg tatttcgcca tgctgccaga acattaatgc tggatgagca
    53221 agcgccgaca ctgcaaatta aagatgtcga tttaacacaa tatgcggata agttaattgc
    53281 acgttttgct aatccgtcgc tgaaacataa gacctggcaa atcgcgatgg atggcagcca
    53341 gaaattaccg caacgtatgc tggcaggtat tcgcatacat ctggggcgtg aaacggactg
    53401 gtcgttgctg gcattaggcg ttgctggctg gatgcgttac gtcagcggcg ttgatgatgc
    53461 cggaaatgcc attgatgttc gcgatccgct tagcgataaa attcgcgaac ttgttgcggg
    53521 cagcagcagt gaacaacgcg taaccgccct gctttccctg cgtgaagttt tcggtgatga
    53581 tctgccagat aacccgcatt ttgtgcaggc catcgaacaa gcctggcaac aaatcgtaca
    53641 attcggcgca catcaggcgc tattaaacac cctcaaaatt taacgatttc tgcggttaaa
    53701 gcggatgaag ctcaccttcg tccgctctcc ccttctcttt tctgcctttt ttagccagga
    53761 ttaacgctca gttaacttac cagagtattg cggagccgtt gtgaccagga ccaacctcat
    53821 caccggtttt ctcggcagcg ggaaaaccac gtcgattctt catctgttag cccataaaga
    53881 tcccaacgaa aaatgggcgg tactggttaa tgaatttggg gaagtcggaa ttgatggtgc
    53941 tttgctcgcc gatagcggcg cattgctgaa agagatcccc ggcggctgca tgtgctgcgt
    54001 taatggttta cccatgcagg tagggttgaa taccttactg cgtcagggaa aaccagaccg
    54061 cttgttgata gagccgaccg ggctgggcca tccgaaacag atcctcgatc tgttaaccgc
    54121 accagtctat gaactgtgga tagatctgcg cgccaccttg tgcattctcg atccacgcct
    54181 gctgctggac gaaaaaagcg ccagcaatga aaacttccgt gaccagctgg ctgccgcaga
    54241 catcattgtc gccaataaat ccgaccgtgc gacgcccgaa agtgagcaag cgctacagcg
    54301 ttggtggcag caaaatggtg gcgatcgaca attaattcac agtgagcatg ggaaagttga
    54361 cggtcatctt ctggatttgc cgcgtcgcaa tttagccgag ttgcccgcca gcgccgcgca
    54421 ttctcatcag catgtcgtga aaaaagggtt agcagcgtta agcctgccag agcatcaacg
    54481 ctggcgtcgc agtctgaaca gcgggcaagg atatcaggcc tgcggctgga tattcgacgc
    54541 tgatacggta ttcgacacca ttggcattct ggaatgggcg cgacttgcac cggtggaacg
    54601 cgtcaaaggc gtgctgcgta ttcccgaagg gctggtacga atcaaccgtc agggcgatga
    54661 cctgcacatt gaaacgcaaa acgttgcgcc accggacagc cgtattgagc tgatttccag
    54721 cagcgaagct gactggaatg ctttgcagag cgcgctgttg aagcttcgtt tagcgactac
    54781 cgcgtaaggt tgcctgcgtt tttcagtaag ataattagag aaaatatgat taaaaatttg
    54841 ccgcaaatag tgttgttgaa tattgtcggc ctcgcgctgt ttctttcctg gtatattccc
    54901 gttaatcatg gattctggtt gccgattgat gcggatattt tttatttctt taatcagaaa
    54961 ctggtcgaaa gtaaggcctt tttgtggctg gttgcattga ccaacaatcg cgctttcgac
    55021 ggttgttctc tgctggcgat gggaatgttg atgctgagtt tctggctgaa agaaaacgcc
    55081 cctggcagac gacgtatcgt gattatgggt ctggtcatgc tattaactgc agtggtatta
    55141 aaccagctgg gtcaggcatt aattcctgta aaacgggcca gcccaacatt gacttttacc
    55201 gatattaacc gcgtcagcga actgctctct gttcccacga aagatgcctc acgagatagc
    55261 tttcccggcg atcacggcat gatgctgctt attttttcgg cattcatgtg gcgttatttc
    55321 ggcaaagttg caggccttat cgcccttatt atttttgtgg tttttgcatt tcccagagta
    55381 atgattggcg cacactggtt tactgacatc attgtcggtt cgatgaccgt gatattgatc
    55441 ggtttgccct gggtgttgct gacgccatta agtgatcgat taatcacctt ttttgacaaa
    55501 tcactaccgg gaaaaaacaa acatttccaa aacaaataac tcacagtaat taacatcatc
    55561 aggcttattt ttatagtgag gataatcctg atgatgcgca ccgtgctttc atccatcgcg
    55621 cgcaaaaatc attctctaag taaatgaatg gattgcatgc gtttcactca atcgtacttt
    55681 aattgaccaa tcccgcttat taactttctg tatcactttt tcttataaaa aatcatgtaa
    55741 aaccgctcgc caagaccgca ccaatcgggt aatctcgaac tcgttttgcc tcggcggtag
    55801 attatcctca cagcatataa ttttgtgcgt tagtccacag atttggcctt aaggaattgt
    55861 ttcaacatgc ccaggtaatt agtctcgtgt cgcttggcat ttttttataa cgatatttgt
    55921 cgttaaggac ttcaagggaa aacaaacaac atggtcaaat ctcaaccgat tttgagatat
    55981 atcttgcgcg ggatccccgc gattgcagta gcggttctgc tttctgcatg tagtgcaaat
    56041 aacaccgcaa agaatatgca tcctgagaca cgtgcagtgg gtagtgaaac atcatcactg
    56101 caagcttctc aggatgaatt tgaaaacctg gttcgtaatg tcgacgtaaa atcgcgaatt
    56161 atggatcagt atgctgactg gaaaggcgta cgttatcgtc tgggcggcag cactaaaaaa
    56221 ggtatcgatt gttctggttt tgtacaacgt acattccgtg agcaatttgg cttagaactt
    56281 ccgcgttcga cttacgaaca gcaggaaatg ggtaaatctg tttcccgcag taatttgcgt
    56341 acgggtgatt tagttctgtt ccgtgccggt tcaacgggac gccatgtcgg tatttatatc
    56401 ggcaacaatc agtttgtcca tgcttccacc agcagtggtg ttattatttc cagcatgaat
    56461 gaaccgtact ggaagaagcg ttacaacgaa gcacgccggg ttctcagccg cagctaataa
    56521 accgtttgga tgcaatccct tggctatcct gacgagttaa ctgaaagcac tgcttaggca
    56581 gtgctttttt gttttcattc atcagagaaa atgatgtttc cgcgtcttga tccaggctat
    56641 agtccggtca ttgttatctt ttaaatgttg tcgtaatttc aggaaattaa cggaatcatg
    56701 ttcatacgcg ctcccaattc tggacgtaag ctcctgctta cctgcattgt agcaggcgtg
    56761 atgattgcga tactggttag ctgccttcag tttttagtgg cctggcataa gcacgaagtc
    56821 aaatacgaca cactgattat cgacgtacaa aagtatctcg atacctattt tgccgacctg
    56881 aaatccacta ctgaccggct tcagccgctg accttagata cctgccagca ggctaacccc
    56941 gaactgaccg ctcgcgcggc gtttagcatg aatgtccgta cgtttgtgct ggtgaaagat
    57001 aaaaaaacat tctgttcatc tgcgactggt gagatggaca ttccactcaa tgaattgatt
    57061 ccggcgctcg acattaataa aaacgtcgat atggcgatct tacccggcac gccgatggtg
    57121 ccgaacaaac ccgcaatcgt catctggtat cgcaaccctt tgctgaaaaa tagcggtgtg
    57181 tttgccgctc tgaatctcaa cctgacgcct tctctctttt atagttcacg gcaggaagat
    57241 tacgatggcg tcgccctcat tattggcaat actgcgctat ctaccttttc ttcacgtttg
    57301 atgaacgtta atgaattaac cgacatgccg gtccgtgaaa ctaaaattgc gggcattcct
    57361 ctgaccgttc ggctttatgc ggatgactgg acatggaacg atgtgtggta cgcattttta
    57421 ctgggcggca tgagtggaac tgtcgttggc ctgctctgct attacctgat gagcgtacgt
    57481 atgcgccccg gcagagaaat catgaccgcc atcaagcgcg aacaatttta cgtggcgtat
    57541 caaccggtgg tggatacaca agctttgcga gtaacgggcc tggaagtact gctacgctgg
    57601 cggcatcctg tcgcgggaga aattcccccg gatgccttca ttaactttgc cgaatcgcaa
    57661 aagatgattg tgccgctgac tcagcacctg tttgagttaa ttgcccgcga tgccgcagaa
    57721 ttagaaaaag tgctgccagt aggcgtcaaa tttggtatta acattgcgcc ggaccatctg
    57781 cacagcgaaa gctttaaagc agatatccag aaactgctca cttccctgcc cgcacaccat
    57841 ttccagattg tgctggaaat taccgagcgc gatatgctga aagagcaaga agccacacaa
    57901 ctcttcgcct ggctgcactc ggtcggcgta gaaattgcta ttgatgactt cggcaccggg
    57961 cacagcgcgc ttatctatct tgagcgtttt acgctcgatt atctgaaaat tgaccgtgga
    58021 tttatcaacg ccatcggtac ggaaacgatc acttcacccg tacttgacgc ggtgctgacg
    58081 ctggcgaaac gcctcaatat gctgacggtt gctgaagggg tcgaaacgcc ggaacaggcg
    58141 cgatggctaa gcgaacgcgg cgttaatttc atgcaaggct actggattag tcgcccttta
    58201 ccgctggacg attttgtccg ctggctgaag aaaccgtata cgccgcagtg gtaaggtgtg
    58261 cttacgtccc ttattattca tagtgaaagc atgccggatt gcggctaatg atgagtaaaa
    58321 ggaaatccat tgcagatgat tgtgcgcata ctgctgctgt ttatcgctct gttcaccttt
    58381 ggtgtgcagg cgcaggctat caaggaaagc tatgcctttg ccgtgctggg cgaaccccgg
    58441 tacgcgttta atttcaacca ttttgattat gtgaaccccg ccgcgccaaa aggtgggcag
    58501 ataacgttgt cagccctcgg caccttcgat aatttcaacc gctatgcact gcgcggcaac
    58561 ccgggcgcac gcaccgagca gctgtacgac acgctattta cgacttccga tgacgaacca
    58621 ggcagttatt acccgctgat tgctgaaagc gcacgctatg ctgacgatta ttcctgggtg
    58681 gaggtcgcta ttaatccgcg cgcccgtttt catgatggtt cgcccattac tgcccgcgat
    58741 gtagagttta cttttcaaaa atttatgacc gaaggcgtgc cgcaatttcg tctggtctac
    58801 aaaggcacca ccgtcaaagc cattgcaccg ttaaccgtgc gcattgagtt agctaaaccc
    58861 agcaaagaag atatgctgag tctgttttcg ctgccgatat ttccagaaaa gtactggaag
    58921 gatcacaaac ttagcgaccc gctcgccacg cctccgcttg ccagtggtcc gtaccgcgtt
    58981 acgtcctgga aaatggggca aaatattgtc tattcccgcg tgaaagatta ctgggcagca
    59041 aacttaccgg taaaccgtgg acgctggaat ttcgacacca ttcgctacga ttattacctc
    59101 gatgataatg tcgcctttga agcgtttaaa gcaggtgcct ttgatttgcg tatggaaaac
    59161 gacgctaaaa actgggccac gcgttatacc ggtaaaaatt tcgataaaaa atacatcatc
    59221 aaagatgagc aaaagaacga atcagcccag gatacgcgct ggctggcgtt taatatccaa
    59281 cgtccggtat tcagcgatcg ccgggtccgg gaagcaatca ctctcgcctt tgactttgaa
    59341 tggatgaaca aggcgttgtt ttacaacgcc tggagtcgca cgaacagtta ttttcagaat
    59401 accgaatacg cggccagaaa ttaccccgac gccgcggagc tggtgcttct ggcaccaatg
    59461 aaaaaagatc taccgccaga agtcttcaca caaatctacc agccgccggt atctaaaggc
    59521 gatggctacg atcgtgacaa cctgttaaaa gccgacaaac ttctcaacga aacgggctgg
    59581 gtgctgaagg gtcagcaacg cgttaatgcc acaacgggtc agccgctcag ctttgaatta
    59641 ttgcttcccg caagcagcaa tagtcagtgg gtattgccgt tccagcacag cctgcaacgg
    59701 ctgggtatca atatggacat tcgcaaggtg gataactcgc aaatcactaa ccgcatgcgc
    59761 agtcgcgact atgacatgat gccgcgccta tggcgggcga tgccgtggcc cagttccgat
    59821 ttacagattt cctggtcatc ggaatatatc aattccactt ataatgcccc cggcgtgcaa
    59881 agcccggtta tcgactcgct gatcaaccaa attattgccg cgcagggaaa taaagaaaaa
    59941 ttactgccgt tggggcgagc actggatcgc gtattaacgt ggaattatta catgctgcca
    60001 atgtggtaca tggcggaaga ccgtctcgcc tggtgggata aattctccca acccgctgta
    60061 cgccctgttt acagcctggg tatcgatacc tggtggtatg acgttaataa agcggccaaa
    60121 ctgccgtcag ccagacaaca gggagagtag atgggcgctt acctgattcg ccgtctgttg
    60181 ctggtgatcc caacgttatg ggctattatc actatcaact ttttcatcgt gcaaattgcg
    60241 cctggcggtc cggtcgatca ggccatcgcc gccattgagt ttggtaatgc cggagtatta
    60301 cccggcgcag gcggtgaagg tgttcgtgcc agccatgcgc aaacgggtgt cggcaatatc
    60361 agcgacagta attaccgtgg cggacgcgga ttagacccag aagtgatcgc tgagatcact
    60421 catcgctacg gtttcgataa gccgatccac gaacgttact tcaaaatgct ctgggactac
    60481 atccgctttg attttggtga tagcctgttt cgcagcgcct cggtgctgac gctgattaaa
    60541 gacagtctgc cggtttccat caccctcgga ttgtggagca cgctgattat ctatctggtg
    60601 tcgattccgt taggcattcg caaagctgtt tataatggga gtcgctttga cgtctggagt
    60661 agcgcattta tcatcatcgg ctacgccatt ccggcctttt tgtttgccat cctgctgatt
    60721 gtcttcttcg cgggcggcag ctatttcgac ctgttccctc tacgcggcct ggtttccgct
    60781 aactttgatt cgctgccgtg gtatcagaaa atcaccgatt atctgtggca tatcacgcta
    60841 ccggtgctgg cgacagtgat tggtggcttt gcggcgctga ccatgctgac caaaaactca
    60901 ttccttgatg aagtgcgcaa gcaatacgtg gtgaccgcgc gtgcgaaagg ggtaagtgag
    60961 aaaaacattc tctggaaaca tgtgttccgc aacgccatgc tgctggtgat tgccggtttt
    61021 ccggcgacgt ttatcagcat gttttttacc ggctcgctgc tgattgaggt gatgttttca
    61081 ctcaatggtc ttggcttact gggctacgaa gcgaccgtct cgcgcgatta tcctgtaatg
    61141 tttggtacct tgtatatttt caccctgatt ggcctgctgc tgaatattgt cagtgatatc
    61201 agctatacgc tggttgatcc gcgtatagat tttgagggac gttaatgtcg cgactcagcc
    61261 ccgtcaatca ggcccgttgg gcgcgttttc gtcataaccg tcgcggctac tggtcgttat
    61321 ggattttcct cgttttgttt ggtttgagtt tgtgttctga acttatcgct aacgataaac
    61381 cgttgctggt gcgttatgac ggcagttggt atttcccgtt gttgaaaaac tacagcgaaa
    61441 gcgattttgg cggcccgctg gcaagtcagg ctgattatca ggacccgtgg ctgaaacaac
    61501 ggctggaaaa taacggctgg gtactgtggg caccgattcg ctttggtgct accagtatca
    61561 actttgctac cgataagccc ttcccttctc caccctcccg gcaaaactgg ctgggaacgg
    61621 atgccaacgg cggcgatgtg ctggcacgca ttctctatgg cacgcggatc tcggttctgt
    61681 ttggcctgat gctgactctc tgttccagcg tgatgggcgt gctggcgggg gcgctacaag
    61741 gctattacgg cggtaaagtc gatctctggg gacaacgctt tattgaagta tggtcgggga
    61801 tgccgacgct gtttttgatt attttacttt ccagcgtcgt acagcctaac ttctggtggc
    61861 tgctggcaat tactgtcttg tttggctgga tgagtctggt cggcgtggtg cgggcggagt
    61921 ttttacgtac tcgtaatttc gactacattc gtgcggcaca ggcgcttggc gtcagcgatc
    61981 gcagtatcat cctgcgtcat atgttgccta atgccatggt cgcgaccctc acctttttac
    62041 cgtttatttt atgtagttcg ataaccaccc tgacctcgct cgatttcctc ggcttcggtc
    62101 taccgctcgg ttcaccgtca ctcggtgaac tgctgttaca agggaaaaat aaccttcagg
    62161 ccccgtggct tgggatcacc gccttcttgt cggtggcgat attattgtct ttgctgatct
    62221 ttattggtga agccgtccgc gacgcatttg atcctaataa ggcggtgtag catgacgcaa
    62281 actctgttag cgattgaaaa tttgtcggtg ggttttcgcc atcagcaaac cgtacgtaca
    62341 gtagtcaatg atgtttcact acagattgag gctggcgaaa cgctggcgct ggtgggtgag
    62401 tcaggttcag gcaaaagcgt taccgcgctg tcaattttac gcctgctccc ttccccgccg
    62461 gttgaatatc tctccggcga tattcgtttt catggcgaat cgctgcttca tgccagcgat
    62521 caaacgttgc gcggtgtacg cggtaataag atcgccatga tttttcagga accgatggtg
    62581 tcgttaaatc cattgcatac cctggaaaaa cagctttatg aagtgctttc actccaccgc
    62641 gggatgcgtc gggaagcggc tcgtggcgaa attcttaact gccttgatcg cgtcggtatc
    62701 cgccaggcgg caaaacggct gacagattat ccgcatcagc tctccggcgg cgaacgccag
    62761 cgggtgatga ttgcgatggc gctgttaacg cgaccggaat tattaattgc cgatgaaccg
    62821 accaccgcac tggacgtctc tgtccaggcg cagattttac agctgttgcg cgaactgcaa
    62881 ggcgagctga atatgggcat gctgtttatt actcataacc tcagcattgt cagaaaactg
    62941 gcccaccgcg tggcggtaat gcaaaacggt cgctgtgtcg agcaaaataa cgccgctacg
    63001 ctatttgcct cacccactca tccttacaca caaaagctac tcaacagtga accgtcaggc
    63061 gatccggtac cgttgccaga acctgcctca acgttgctgg atgttgaaca gcttcaggtt
    63121 gccttcccca ttcgcaaagg gattttgaag cgcattgtgg atcataatgt ggtggtgaaa
    63181 aacatcagtt ttacgctacg ggcgggtgaa acactgggtt tagtgggcga gtctggttcc
    63241 gggaaaagca cgacgggact ggcgctgctg cgactgatta cctctcaggg cagcatcgtc
    63301 tttgacggtc agccactgca agatttaaat cgccgccagc tgttacctac tcgtcatcgt
    63361 attcaggtgg tatttcagga tccaaactcc tcgctcaacc cacgactcaa cgttttgcag
    63421 attattgagg aaggcttacg ggttcaccag ccgacgcttt ctgccgcaca acgcgaacaa
    63481 caagtgatag ccgtgatgca tgaagtggga ttagatcctg aaacacgcca ccgttatccg
    63541 gcggagttct ctggtggtca gcgacaacgt attgcgattg ccagggcatt aattcttaag
    63601 ccctcactga tcatacttga tgaaccgaca tcatcactcg acaaaacggt acaggcgcaa
    63661 atattgacgc tattgaaatc attgcaacaa aagcatcaac tggcctattt gtttatcagc
    63721 cacgatttgc acgttgtccg cgcgttatgt catcaggtta tcgtactgcg acaaggggaa
    63781 gtagtggaac aaggaccgtg cgcgcgcgtg tttgccgcac cgcagcagga gtatacgcgt
    63841 cagctactgg cgttgagctg acgcttaaaa aggattgtag tttgaaaagg gttcggcaat
    63901 tgccacacca aaatttttca atcggcatgt tgcagcaaac tcgtcctggc gatttacaaa
    63961 caggcacggc tcaccttcac actccagcac cgaacatgga acttcgatgt cgcttaacgc
    64021 ctggcttagt ttatacatca ccgaccatgc attatcacca tccgggctaa gcaatttaag
    64081 cgccaccagg ctgttgtcac cgcacggtcc attttgcgga atcggttcaa ccttcgaacc
    64141 tgcgaaacca gcagaccagc gaaagttctg cggtagacga tggacgattg agagttgtaa
    64201 tgaagtcact tattttcccc ggaagcacat ttacttcaca atttatttac atcaatttta
    64261 acacatcatc aacaatccgt ctttatacag atcgtaaaga ttgatgcctc gcctgcatgg
    64321 cctctatggc tctgatttaa gtaatatctg cgggcgtggt cacgtttgcg gggctatatg
    64381 tacccgtttc tgcgatctaa ctcaaccttt ttaactacaa tgatgtgact ttttacataa
    64441 attgatttta cataaaataa acatatatcg ggggaatgat agatttgtgg ttgacatgca
    64501 tcaacaaagg aagcctttta gcttcctcgt tgtgcaatag atcaccgttt tttcggtcga
    64561 ctggcgtaca gacagaagag aatggagctg gttgcgcaga atgcaattga ccaaatcatc
    64621 ggccatgcag agttaaaggt cgcaagagaa agcaatgcgc caacaattgc ccctatgcca
    64681 aaacggaagg ttcctgccag cgaagatgcc gttcccgcca tatggggaaa ctcatcaaga
    64741 atgaccgcca tcgcattgga tgacaccata gacacgcagc ccacaaacgc cgcaacgcca
    64801 accaccagcg accagaatcc cagccccagc agcgcactga tgaccatcca cgctgccata
    64861 ataaattgta tccacaaccc cgagcggaac atatttaacg cgccaatgcg gcggacgaag
    64921 cggctgttaa agatggtcat cacgaacaga aaaacaatgt ttagcgcaaa gtaataacca
    64981 aagttttccg gtgcaacgtg gttgatttca atgtagacaa acggtccggc gctcaggaac
    65041 gagaacattc cggcaaagct gaaaccgctg gccagcatat aactcaggac acgcttatga
    65101 cggaacagcg ccgcaaagtt accaatagtg gtacgaatgt gaaaaggctg gcgaggatcc
    65161 agaagatgta atgccagctc agccacacca gcacccagcc gccaactatc ggtgccatca
    65221 gcggtgcaat ggtcgtcacc agcatgacaa acgacatcat ccgcgagaac tcttctttcg
    65281 ggtaaatatc acgcatcaaa gcgttgataa ccacgctggc cgcagccgca gccagcccgt
    65341 ggaagaaacg catcacaatc agctgatcga tggtttgtgc caacgcacac gccaccgcgg
    65401 cggcggcaaa caccagcgta ccgccaagaa ccaccggctt acgcccgaag ctgtctgcca
    65461 tcggcccgta gattaactgc cccaacgcaa agcccagaat ataagtactc agggtcatct
    65521 gcgtactgcc cgccggtacg ccaaactgcg ctgaaattac cggtagcgcg ggcagataca
    65581 tatcaatcga cagcggcatc aacatggcca gcaggccaag gataaaaaca atagcaaacg
    65641 acgaatgctg tcgggtggtc acaacgggct cctgaaagtc attgaaaagt tagacgacgc
    65701 tggcaatttc ttcttcagtt aacggacgat attcaccggg ggctaaatca gcatccagcg
    65761 taatgccgcc aatacgttca cgatgcagct caaccacatg gttacccacg gcggcgaaca
    65821 tgcgtttcac ctgatgataa cgcccttcgc tgatggtcag acgaacctgc gttggggtaa
    65881 tcacttccag caccgccggc ttagtgagat ctttttcgtt atgcagctgc acgcctttag
    65941 caaattgttc tgccgtatcg tcagctacgg gtgataccag cgtcaccaga taggtcttct
    66001 cgcaatgatg gcgcggagaa gtaatgcggt gcgaccactg accatcatct gtcattagta
    66061 ccagaccggt ggtatcgata tccagccgcc ctgcggcgtg cagtttccac gctaccggtt
    66121 catctagaaa atagagcacc gttgggtgat cagggtcgtc cgtggagcaa acatagccct
    66181 gaggcttatt gagcatgaag taacgtggac cgtgttgctg cgccagcggg ttgccatcgt
    66241 aagcgacatc atgttcagga agcagtttga acgctgcatt acggacgatt tcgccatcga
    66301 cggtgacacg attgccgcgg atttcacgcc cggcaatagc acggctaacg ccgagttgct
    66361 gtgcgataaa tttatcaagt cgcatgtgtg tgattttgcc tgtaaaaaac ggaggtcggg
    66421 taatgacccg aatatctgaa ctgttgtctg cccagtatag cggtctaata acatccctca
    66481 agggaaaaag atccgtggca tactattagc agaataatct acctacgcga gaccatgatt
    66541 tttacacttc gcccatatca gcaagaagcc gtggatgcca cgctcaacca ttttcgtcgt
    66601 cataaaaccc ctgccgttat cgtgctgccc accggtgcag gtaaaagcct ggtgatagcg
    66661 gaactggcac ggctggctcg tggtcgcgta ctggtgctgg cacacgttaa agaactggtg
    66721 gcgcaaaacc atgcaaagta tcaggcgctg gggctggaag ccgatatttt tgccgccggg
    66781 ctgaagcgta aagagagcca cggtaaagtg gtatttggca gtgtgcagtc ggtcgcccgt
    66841 aatattgatg cctttcaggg tgaattttcg ctgttgattg tcgatgaatg tcaccgtatt
    66901 ggtgacgatg aagagagcca gtatcagcaa atcctcactc acctgacaaa agtgaatccc
    66961 cacttacgcc tgctggggct gactgccacg ccttttcgat tgggcaaagg ttggatctac
    67021 cagttccatt atcacggcat ggtacgcagc gatgagaaag cccttttccg tgactgcatt
    67081 tatgagctgc ccctgcgtta tatgattaaa cacagctatc tgacgccgcc agaacgactg
    67141 gatatgccag tagtgcaata tgatttcagc cgcttgcagg cacagagcaa cgggctgttc
    67201 agcgaagccg atctcaaccg tgagctgaaa aaacagcaac gtattacccc gcacatcatc
    67261 agccagatta tggagttcgc cgaaaagcgt aaaggagtga tgattttcgc cgcgacggtc
    67321 gaacacgcaa aagagattgt gggattactg cctgccgaag atgcagcact gattactggc
    67381 gacacccccg gcgctgagcg cgatgtgtta attgaaaatt ttaaagccca gcgttttcgc
    67441 tatctggtca acgtcgcggt actgaccacc ggatttgacg ccccgcacgt cgatcttatc
    67501 gccattctgc gccctaccga atcagtgagt ctttaccaac aaattgtcgg gcgcggtctg
    67561 cgtctcgctc cgggcaagac tgattgttta attcttgatt atgcgggtaa tcctcacgat
    67621 ctctacgcgc cggaagttgg tacaccgaaa ggcaaaagtg acaacgttcc ggtacaggtt
    67681 ttctgccctg cctgcggttt tgccaacacc ttttggggga aaacgaccgc cgacgggaca
    67741 ttgattgaac actttggtcg ccgctgtcag ggatggtttg aagatgacga cggtcatcgc
    67801 gaacagtgtg acttccgttt ccgttttaaa aattgcccgc aatgtaacgc agaaaatgat
    67861 attgccgctc gccgctgccg cgaatgtgac acggtactgg ttgatccgga tgatatgtta
    67921 aaagcggcgc tacgactgaa agatgcgctg gtattacgct gtagcggcat gtctttgcag
    67981 catgggcacg acgagaaagg cgaatggttg aaaatcacct attacgatga agacggtgcg
    68041 gatgtgagtg agcgtttccg tctgcaaaca cctgcccagc gtaccgcctt cgagcagctt
    68101 tttatccgcc cgcatacgcg cacaccgggc atcccgctgc gctggatcac cgccgccgat
    68161 atcctcgccc agcaagcctt attgcgaccc ccggattttg tcgtcgcccg catgaaaggc
    68221 cagtactggc aggtgcgtga aaaagtgttc gattacgaag gtcgttttcg tcgggcgcac
    68281 gaattacgcg gttaatggta gttttgattg atgtataagg cgattgagta tagaatctcg
    68341 cccgcttttg catacgcaaa gcagatcact tacctgttgc tgggtcgcct gtagcaggat
    68401 taatttaaga gagaaagaaa tgtttactat caacgcagaa gtacgtaaag agcagggtaa
    68461 gggtgcgagc cgccgcctgc gtgccgctaa caagttcccg gcaatcatct acggtggcaa
    68521 agaagcgccg ctggctatcg agctggatca cgacaaagtc atgaacatgc aagctaaagc
    68581 tgaattctac agcgaagttc tgaccatcgt tgttgacggt aaagaaatca aagttaaagc
    68641 tcaggacgta cagcgtcacc cgtacaaacc gaagctgcag cacatcgact tcgttcgcgc
    68701 ttaattgctg aataagttgt aaaaaacccc gctccggcgg ggttttttgt atctgcagat
    68761 tatgcctgat tacggtattg ctattttttg caggccagat aaggcgttca cgccgcatct
    68821 ggcattcaga acacaaaacc gattaattgc cgccagacgt ccggcgctgc aattggtcgc
    68881 gcaaattcgg cggcgtgcct ttaatggtca gggtatcggt cgccggatcc cagaagatgc
    68941 gttcacctaa cagcatcgca tcaaagttaa tagtcaagcc accgccgcta ccggcaaact
    69001 tcgtcaactg acgcagtgtg ctccggtctg ccgggaagct ctcttccagt tcgtagcctt
    69061 tttcagccgc aaactccgtg aagctcactt cgcttacgcc tgccagctct ttcgacagcg
    69121 attccagttc gatctcttcg cctgcctgca gctgctcatt acagtagcta taaacctgct
    69181 ggcgcacgtt ctgtcgctct gctttatcca gttgtgcttc ggcagtgaaa tcatcaaccg
    69241 cttgcaacag cccacggttt tgcgctttcg tgttcaatcc ttcgctggcc ccgaggaaat
    69301 ccataaagaa atccgccact ttgcgcccta ctcgcccttt caggaaagtg agatagcggg
    69361 tggactctgg attggtttcc cattcggtta aatcgatacg cgcaacaata tccgcatgat
    69421 tgatatcaag ataatgggtt gggttgatgt cgagattttc gttaacacgc atactgctca
    69481 ggttgctcag aactgccacc agcagatact ccaccgccag ataacggtag tggcaaaaca
    69541 gcacgaatcc accgtcagca aaaggatatt tcgctaattc gtcacgcagg cgaccagttg
    69601 ccgcgcggct aaatgccaga aagtcctctt cgccctgacg ctgtaagcgc agcgtctgcg
    69661 ccagttcact ctcttcgcta aacaagccgt aagctttatt tttggcgcta tagacccgat
    69721 gcagttctgc caccatctca acgacggttt ctgtcggttc cagcaatgaa tcgcgcaaca
    69781 ccagctcaag gttctgctca tcacgcttga taagctggtg cagagcaatc tggttgatat
    69841 ccagactcat gataaactct cctttaagac cgggcggtat tcaaccaccg ccggttaagc
    69901 gacgcaataa aagatggcgg tcattttaca gaaggcagcg taacgagaat gatttaacgc
    69961 ataaaaaagc agaaaaaaaa ccgttgctac ggtaatatgt tgccctttca tcaactaact
    70021 gatttcgatt tatgccacaa atttcccgct acagtgatga acaggttgaa caattgctcg
    70081 cagagctgct caacgtactg gaaaaacata aggctccgac cgacctttcc ctgatggtgt
    70141 taggcaacat ggtcactaac cttatcaaca ccagcattgc cccggcccaa cgccaggcaa
    70201 ttgccaactc ttttgcccgc gccttacagt cctctatcaa cgaagacaaa gcgcactaag
    70261 ggaaacagat aacaggttat ggtaactcat cgtcagcgct accgtgaaaa agtctcccag
    70321 atggtcagtt gggggcactg gtttgcactg ttcaatattc tgctttcgct cgtcattggc
    70381 agccgttacc tgtttatcgc cgactggccg acaacgcttg ctggtcgcat ttattcctac
    70441 gtaagcatta tcggccattt cagcttcctg gtgttcgcca cctacttgct gatcctcttc
    70501 ccgctgacct ttatcgtcgg ctcccagagg ctgatgaggt ttttgtccgt cattctggca
    70561 acggcgggaa tgacgctatt actgatcgat agcgaagtct ttactcgttt ccatctccat
    70621 cttaatccca tcgtctggca gttggttatc aacccagacg aaaatgagat ggcgcgcgac
    70681 tggcagctga tgttcatcag cgtgccggtt attttattgc ttgaactggt gtttgcgacg
    70741 tggagctggc aaaagctgcg cagcctgacg cgtcgtcgac gcttcgcgcg cccgctggcc
    70801 gcattcttat ttatcgcctt tatcgcctcg catgtggtgt atatctgggc cgatgccaac
    70861 ttctatcgcc cgatcaccat gcagcgcgct aacctgccgc tttcgtaccc gatgacggcg
    70921 cgacgttttc ttgagaagca tggtctgctt gatgcgcagg agtatcaacg ccgtcttatt
    70981 gagcaaggta atccagacgc cgtttccgtt cagtatccgt taagcgaact gcgctatcgc
    71041 gatatgggca ccgggcagaa tgtgctgttg attactgtcg atggcctgaa ctactcacgc
    71101 ttcgagaagc agatgcctgc gctggcaggt tttgctgagc aaaatatttc gttcacgcgc
    71161 catatgagct ccggcaacac tacagacaac ggcatctttg gcctgttcta tggtatctcg
    71221 ccgagctata tggacggcat tctgtcgacc cgtacgcctg cggcattaat tactgcgctt
    71281 aatcagcaag gctatcagct ggggttattc tcatcagatg gctttaccag cccgctgtat
    71341 cgccaggcat tgttgtcaga tttctcgatg ccgagcgtac gcacccaatc cgacgagcag
    71401 accgccacgc agtggatcaa ctggctgggc cactacgcac aagaagataa ccgctggttc
    71461 tcgtgggttt ctttcaatgg cactaacatt gacgacagca atcagcaggc atttgcacgg
    71521 aaatatagcc gggcggcagg caatgtcgat gaccagatca accgcgtgct caatgcactg
    71581 cgtgattctg gcaaactgga caatacggtt gtgattatca ctgccggtcg gggtattccg
    71641 ctgagcgaag acgaagaaac ctttgactgg tcccacggtc atctgcaggt gccattagtg
    71701 attcactggc caggcacgcc ggcgcagcgt attaatgcgc tgactgatca taccgatctg
    71761 atgacgacgc tgatgcaacg cctgctacat gtcagcacac ctgccagcga atattcgcaa
    71821 ggtcaggatt tgttcaaccc tcaacgccgt cattactggg ttactgcagc ggataacgat
    71881 acgctggcaa ttaccacccc gaaaaagacg ctggtgctga acaataacgg taaataccgc
    71941 acttacaact tacgtggtga aagagtgaaa gatgaaaaac cacagttaag tttgttattg
    72001 caagtgctga cagacgagaa gcgttttatc gctaactgat taattataaa tcagttagcg
    72061 aaatatctta cttgcaatcg gtgtggaaaa cggtagtatt agcagccacg agtcggcacg
    72121 tagcgcagcc tggtagcgca ccgtcatggg gtgtcggggg tcggaggttc aaatcctctc
    72181 gtgccgacca aaaatcccaa gaaaaaacca acctttacgg ttggtttttt tatatctgca
    72241 attaatttga taaacagacc gtgacacatc acagcctgtt tattttctgt tatcagaacg
    72301 tccagaccac tcccgcctga gtattccacg gcgattctac ccctgcgcca ttgccatagc
    72361 tgactgacaa atgaccgctt aacgtcgggg taaacgatga ccttatccca gcctgataaa
    72421 cgccacgggt tccagccaca tcattattaa atttgccatc gtcattaact ttcacatggt
    72481 tagaatcggc gtattcctga cgcacggcgg ctttcagcca gggttccaac gtcgtaccgt
    72541 tttgcaggtc catgtgatag cttaccgccg ttcccgcttc agcgcgtaat atccgggtat
    72601 ttcccacatc cgcgcgcatg ccgtttgata acgtgtagtc ctgaccatct gtggtaaagc
    72661 cggtaaaggc gagatagggt ctaacactcc acaatccgtc aacccaacgg aacccgctct
    72721 caacatgagc acccgcgccg ttactattgt aatcgccaaa cgctgttgcc ccattactca
    72781 tcttgccatg gatggtgttg gcaaaacggt caactttcac caccccatca acataggcac
    72841 cgttctgatg ctcccaaccg gcataagccc ccagggtgta gctatcaata ttgcctttgc
    72901 cgccgcgatc aaaaccaata tcagaatgag agtaatcaaa gaacaagccg cgaattgtgc
    72961 tgctttcttc acgggagaaa cggctatcga taccgagcgt caggcccgtc aatgtttgct
    73021 caaagccagc tcccgcatca gtggtcacgt tgttgcgggt gttaattgcc gaactccaca
    73081 tcgccgtatc gtaattaacg ccttttacgc taccaagacg ctcacgcacg gtgtccagtt
    73141 ctgcatcaaa taccagcggt tgtgctgctg ccatgttcag cacatcagtg gttgaagggg
    73201 taatttgcgc gcgattctct gcgaggctcc agctatggtt gccattatcc agcaaggtat
    73261 attcatacgt accgatatca acaacgcctc cggcattgcc caacgtgaat gcagcatcac
    73321 cgccgcccgt cgttaccagt gtaaggctat ctcctgctgc cgggctggcc cccgtgtccg
    73381 tcacgaatat tttgaaatca cctgttgcct gaccgatgac gttgagctga tcgctctgat
    73441 gattagccat atcggtacgc atataaaatt gccgtttccc gacagggtat tggtggtcaa
    73501 cgtaatgaaa ttttccgcac ttgccgtcac agatgaacga gtcactggct cagccatcat
    73561 aatcacattt gcgccggaca tcgcaaggtt ggctaagttg aggcgagtgt tttggttcgt
    73621 cgtagtgata tcggcaatga tggtccatgc gctattttcg agcgaaacgt ccgcttggct
    73681 ggtatccacg gcaccatgcg ttctaaaaga agcgccctca gaaaccacta ccttacccac
    73741 caacatagcg ggtgcataat ttatttgcgg cttcatgacc tcaagaatgc catcattccc
    73801 tctgactgac acgttaacca ttgtgccagc ccagacatta gcgcggccat tgttgataac
    73861 catattttca gagatggctt tagaggaata cgtgatactt ccattctgat ctga
//
LOCUS       DAKISA010000006        72510 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.006, whole genome shotgun sequence.
ACCESSION   DAKISA010000006 DAKISA010000000
VERSION     DAKISA010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72510
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.006"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..215)
                     /gene="tuf"
                     /locus_tag="OVW45_000391"
     CDS             complement(<1..215)
                     /gene="tuf"
                     /locus_tag="OVW45_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCV5524926.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(286..2400)
                     /gene="fusA"
                     /locus_tag="OVW45_000392"
     CDS             complement(286..2400)
                     /gene="fusA"
                     /locus_tag="OVW45_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCV5524927.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="OVW45_000393"
     CDS             complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="OVW45_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCV5524928.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="OVW45_000394"
     CDS             complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="OVW45_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCV5524929.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="OVW45_000395"
     CDS             complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="OVW45_000395"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCV5524930.1"
                     /translation="MLHTLHRSPWLADFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="OVW45_000396"
     CDS             complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="OVW45_000396"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCV5524931.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="OVW45_000397"
     CDS             complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="OVW45_000397"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCV5524932.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4604..5326)
                     /locus_tag="OVW45_000398"
     CDS             complement(4604..5326)
                     /locus_tag="OVW45_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCV5524933.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="OVW45_000399"
     CDS             complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="OVW45_000399"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCV5524934.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6526..6744
                     /locus_tag="OVW45_000400"
     CDS             6526..6744
                     /locus_tag="OVW45_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCV5524935.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="OVW45_000401"
     CDS             complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="OVW45_000401"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCV5524936.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7478..7678)
                     /locus_tag="OVW45_000402"
     CDS             complement(7478..7678)
                     /locus_tag="OVW45_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCV5524937.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="OVW45_000403"
     CDS             complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="OVW45_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HCV5524938.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="OVW45_000404"
     CDS             complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="OVW45_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCV5524939.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10175..12088
                     /locus_tag="OVW45_000405"
     CDS             10175..12088
                     /locus_tag="OVW45_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCV5524940.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12088..13110
                     /locus_tag="OVW45_000406"
     CDS             12088..13110
                     /locus_tag="OVW45_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCV5524941.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13104..13322
                     /locus_tag="OVW45_000407"
     CDS             13104..13322
                     /locus_tag="OVW45_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCV5524942.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13376..14245
                     /locus_tag="OVW45_000408"
     CDS             13376..14245
                     /locus_tag="OVW45_000408"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCV5524943.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14300..14704)
                     /locus_tag="OVW45_000409"
     CDS             complement(14300..14704)
                     /locus_tag="OVW45_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCV5524944.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15006..15638
                     /gene="crp"
                     /locus_tag="OVW45_000410"
     CDS             15006..15638
                     /gene="crp"
                     /locus_tag="OVW45_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCV5524945.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15689..17779
                     /locus_tag="OVW45_000411"
     CDS             15689..17779
                     /locus_tag="OVW45_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCV5524946.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDSPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17846..19066)
                     /gene="argD/dapC"
                     /locus_tag="OVW45_000412"
     CDS             complement(17846..19066)
                     /gene="argD/dapC"
                     /locus_tag="OVW45_000412"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCV5524947.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINPPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="OVW45_000413"
     CDS             complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="OVW45_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCV5524948.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19747..20349)
                     /locus_tag="OVW45_000414"
     CDS             complement(19747..20349)
                     /locus_tag="OVW45_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCV5524949.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20339..20506)
                     /locus_tag="OVW45_000415"
     CDS             complement(20339..20506)
                     /locus_tag="OVW45_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCV5524950.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="OVW45_000416"
                     /gene_synonym="rotA"
     CDS             complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="OVW45_000416"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCV5524951.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21454..22635
                     /gene="tsgA"
                     /locus_tag="OVW45_000417"
     CDS             21454..22635
                     /gene="tsgA"
                     /locus_tag="OVW45_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCV5524952.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22897..25440
                     /gene="nirB"
                     /locus_tag="OVW45_000418"
     CDS             22897..25440
                     /gene="nirB"
                     /locus_tag="OVW45_000418"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCV5524953.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25437..25763
                     /gene="nirD"
                     /locus_tag="OVW45_000419"
     CDS             25437..25763
                     /gene="nirD"
                     /locus_tag="OVW45_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCV5524954.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25889..26695
                     /gene="nirC"
                     /locus_tag="OVW45_000420"
     CDS             25889..26695
                     /gene="nirC"
                     /locus_tag="OVW45_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCV5524955.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHAMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26714..28087
                     /gene="cobA"
                     /locus_tag="OVW45_000421"
     CDS             26714..28087
                     /gene="cobA"
                     /locus_tag="OVW45_000421"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCV5524956.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28334..28501
                     /locus_tag="OVW45_000422"
     CDS             28334..28501
                     /locus_tag="OVW45_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCV5524957.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28745..30133
                     /gene="frlA"
                     /locus_tag="OVW45_000423"
     CDS             28745..30133
                     /gene="frlA"
                     /locus_tag="OVW45_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HCV5524958.1"
                     /translation="MTANSPLQRIGQEKGIAMGSQELQRKLGFWAVLAIAVGTTVGSG
                     IFVSVGEVAKAAGTPWLTVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGS
                     RPLAFLSGWASFWANDAPSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHL
                     RSVEGGAAFQTLITIAKIIPFTIVIGLGIFWFKAENFDAPTTTAIGATGSFMALLAGI
                     SATSWSYTGMASICYMTGEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKL
                     ANSETPISDALTWIPALGSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFF
                     KCFGHVHPKYNTPDVSIILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWC
                     RKRDDYKPLWRTPAFGLMTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAF
                     WAKRSRQLNALS"
     gene            30154..31176
                     /gene="frlB"
                     /locus_tag="OVW45_000424"
     CDS             30154..31176
                     /gene="frlB"
                     /locus_tag="OVW45_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCV5524959.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKPLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31226..32057
                     /gene="frlC"
                     /locus_tag="OVW45_000425"
                     /pseudo
     CDS             31226..32057
                     /gene="frlC"
                     /locus_tag="OVW45_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
     gene            32054..32839
                     /gene="frlD"
                     /locus_tag="OVW45_000426"
     CDS             32054..32839
                     /gene="frlD"
                     /locus_tag="OVW45_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCV5524960.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TLVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32939..33670
                     /gene="phoB"
                     /locus_tag="OVW45_000427"
     CDS             32939..33670
                     /gene="phoB"
                     /locus_tag="OVW45_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCV5524961.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33822..34907)
                     /locus_tag="OVW45_000428"
     CDS             complement(33822..34907)
                     /locus_tag="OVW45_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCV5524962.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTQVIIEQMGLTLITADFNDLSALKQVVDEQLPDAALVQHTRQQPQDGYILADVLATL
                     RSAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRS
                     TLYSGGSQIQGAQALEVLCGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRNGEETVLRILRESIASI"
     gene            complement(34919..36223)
                     /locus_tag="OVW45_000429"
     CDS             complement(34919..36223)
                     /locus_tag="OVW45_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCV5524963.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPIVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36235..36588)
                     /locus_tag="OVW45_000430"
     CDS             complement(36235..36588)
                     /locus_tag="OVW45_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCV5524964.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36599..37477)
                     /locus_tag="OVW45_000431"
     CDS             complement(36599..37477)
                     /locus_tag="OVW45_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCV5524965.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLGVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            complement(37474..38700)
                     /locus_tag="OVW45_000432"
                     /pseudo
     CDS             complement(37474..38700)
                     /locus_tag="OVW45_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
     gene            complement(38700..39863)
                     /locus_tag="OVW45_000433"
                     /pseudo
     CDS             complement(38700..39863)
                     /locus_tag="OVW45_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
     gene            complement(39947..40309)
                     /locus_tag="OVW45_000434"
     CDS             complement(39947..40309)
                     /locus_tag="OVW45_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCV5524966.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(40326..41231)
                     /locus_tag="OVW45_000435"
     CDS             complement(40326..41231)
                     /locus_tag="OVW45_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5524967.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41391..42395)
                     /gene="trpS"
                     /locus_tag="OVW45_000436"
     CDS             complement(41391..42395)
                     /gene="trpS"
                     /locus_tag="OVW45_000436"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCV5524968.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42388..43146)
                     /gene="gph"
                     /locus_tag="OVW45_000437"
     CDS             complement(42388..43146)
                     /gene="gph"
                     /locus_tag="OVW45_000437"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCV5524969.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43139..43816)
                     /gene="rpe"
                     /locus_tag="OVW45_000438"
     CDS             complement(43139..43816)
                     /gene="rpe"
                     /locus_tag="OVW45_000438"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCV5524970.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43834..44670)
                     /gene="dam"
                     /locus_tag="OVW45_000439"
     CDS             complement(43834..44670)
                     /gene="dam"
                     /locus_tag="OVW45_000439"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCV5524971.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44777..46063)
                     /gene="damX"
                     /locus_tag="OVW45_000440"
     CDS             complement(44777..46063)
                     /gene="damX"
                     /locus_tag="OVW45_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCV5524972.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPTATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETARNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46155..47243)
                     /gene="aroB"
                     /locus_tag="OVW45_000441"
     CDS             complement(46155..47243)
                     /gene="aroB"
                     /locus_tag="OVW45_000441"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCV5524973.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47300..47821)
                     /gene="aroK"
                     /locus_tag="OVW45_000442"
     CDS             complement(47300..47821)
                     /gene="aroK"
                     /locus_tag="OVW45_000442"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCV5524974.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48222..49460)
                     /gene="hofQ"
                     /locus_tag="OVW45_000443"
     CDS             complement(48222..49460)
                     /gene="hofQ"
                     /locus_tag="OVW45_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCV5524975.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49372..49776)
                     /gene="hofP"
                     /locus_tag="OVW45_000444"
     CDS             complement(49372..49776)
                     /gene="hofP"
                     /locus_tag="OVW45_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HCV5524976.1"
                     /translation="MRVKRWLLAGIVLCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(49766..50206)
                     /gene="hofO"
                     /locus_tag="OVW45_000445"
     CDS             complement(49766..50206)
                     /gene="hofO"
                     /locus_tag="OVW45_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCV5524977.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(50190..50729)
                     /gene="hofN"
                     /locus_tag="OVW45_000446"
     CDS             complement(50190..50729)
                     /gene="hofN"
                     /locus_tag="OVW45_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCV5524978.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTD
                     NAEARVNTVLLQAEQQLARSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50729..51508)
                     /gene="hofM"
                     /locus_tag="OVW45_000447"
     CDS             complement(50729..51508)
                     /gene="hofM"
                     /locus_tag="OVW45_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCV5524979.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRCRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51628..54180
                     /gene="mrcA"
                     /locus_tag="OVW45_000448"
     CDS             51628..54180
                     /gene="mrcA"
                     /locus_tag="OVW45_000448"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCV5524980.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMDGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGVGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54345..54905)
                     /gene="nudE"
                     /locus_tag="OVW45_000449"
     CDS             complement(54345..54905)
                     /gene="nudE"
                     /locus_tag="OVW45_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCV5524981.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55225..57360
                     /gene="igaA"
                     /locus_tag="OVW45_000450"
     CDS             55225..57360
                     /gene="igaA"
                     /locus_tag="OVW45_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCV5524982.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDGSRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            57425..58093
                     /gene="yrfG"
                     /locus_tag="OVW45_000451"
     CDS             57425..58093
                     /gene="yrfG"
                     /locus_tag="OVW45_000451"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCV5524983.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58104..58505
                     /gene="hslR"
                     /locus_tag="OVW45_000452"
     CDS             58104..58505
                     /gene="hslR"
                     /locus_tag="OVW45_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCV5524984.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58530..59408
                     /gene="hslO"
                     /locus_tag="OVW45_000453"
     CDS             58530..59408
                     /gene="hslO"
                     /locus_tag="OVW45_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCV5524985.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(59543..60916)
                     /locus_tag="OVW45_000454"
     CDS             complement(59543..60916)
                     /locus_tag="OVW45_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCV5524986.1"
                     /translation="MAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNG
                     LTWLVLLLWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALVVILARTQSRLIDSIQ
                     KLFTLIATGLLPLVSLLTLMFIITLPFTDLSAISRHISAAGLLLTLAFLQLILMAIVR
                     DPQKASLPWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVL
                     LVWSLGYFVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMA
                     RYQSGKNTPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQ
                     EQVSEKVLAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQA
                     ERILFAFNDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLDLTVDGET
                     LEINLSK"
     gene            complement(60929..61243)
                     /locus_tag="OVW45_000455"
     CDS             complement(60929..61243)
                     /locus_tag="OVW45_000455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCV5524987.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FG"
     gene            61622..63244
                     /gene="pckA"
                     /locus_tag="OVW45_000456"
     CDS             61622..63244
                     /gene="pckA"
                     /locus_tag="OVW45_000456"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCV5524988.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63320..64672)
                     /gene="envZ"
                     /locus_tag="OVW45_000457"
     CDS             complement(63320..64672)
                     /gene="envZ"
                     /locus_tag="OVW45_000457"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCV5524989.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64669..65388)
                     /gene="ompR"
                     /locus_tag="OVW45_000458"
     CDS             complement(64669..65388)
                     /gene="ompR"
                     /locus_tag="OVW45_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCV5524990.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65616..66092
                     /gene="greB"
                     /locus_tag="OVW45_000459"
     CDS             65616..66092
                     /gene="greB"
                     /locus_tag="OVW45_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCV5524991.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVNYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            66189..68510
                     /locus_tag="OVW45_000460"
     CDS             66189..68510
                     /locus_tag="OVW45_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCV5524992.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKVAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            68948..69175
                     /gene="feoA"
                     /locus_tag="OVW45_000461"
     CDS             68948..69175
                     /gene="feoA"
                     /locus_tag="OVW45_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCV5524993.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69192..71513
                     /gene="feoB"
                     /locus_tag="OVW45_000462"
     CDS             69192..71513
                     /gene="feoB"
                     /locus_tag="OVW45_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCV5524994.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTSARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71513..71749
                     /gene="feoC"
                     /locus_tag="OVW45_000463"
     CDS             71513..71749
                     /gene="feoC"
                     /locus_tag="OVW45_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCV5524995.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            71952..>72510
                     /locus_tag="OVW45_000464"
     CDS             71952..>72510
                     /locus_tag="OVW45_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HCV5524996.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVLLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQR"
BASE COUNT        17432 a        19701 c        18354 g        17023 t
ORIGIN      
        1 gtgtcatatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc acgagcagca ccgccgtagg ttttagccag tacggtggtg
      121 attgcagcgg tcagagtagt tttaccgtgg tcaacgtggc cgatagtacc aacgttaacg
      181 tgcggttttg tacgttcaaa tttttcttta gacacggcta tattccttac tatagtgctc
      241 tccccttcag gagagagcac gggactttgg tattaaccct taggcttatt taccacgggc
      301 ttcaattacg gcctgagcaa cgttactcgg cgcttcatca tacttcagga attccatagt
      361 gtatgatgca cgacctttgg tcagagaacg cagctgagtt gcgtatccga acatttcaga
      421 cagcggtact tcagcgtgga tcttaacgcc agtaacttca gattcctgac ctttgagcat
      481 accacgacga cggctcaagt caccgataac gtcaccggtg ttctcttccg gagtttctac
      541 ttcaaccttc atgatcggct caagcagaac tggtttcgct ttcttaaagc cttctttaaa
      601 ggcgatagaa gcagccagtt taaacgccag ttcagaggag tcaacgtcat ggtaagaacc
      661 gaagtgcaga cgaataccca tgtctactac cgggtagcct gccagcggac ctgctttcag
      721 ctgttcctgg atacctttat caacggccgg gatgtattcg ccagggatta caccaccttt
      781 aatgtcgttg atgaactcgt agcctttcgg gtttgaaccc ggctccagcg ggtacatgtc
      841 gataacaaca tgaccatact gaccacgacc accagactgt ttcgcgtgtt taccttcaac
      901 atcggtaact ttctggcgga tagtttcacg gtaagcaacc tgcggtttac ctacgttcgc
      961 ttcaacgttg aattcacgct tcatacggtc aacgatgatg tcgaggtgca gttcgcccat
     1021 acccgcgatg atggtctggt tagattcttc gtcagtccat acacggaaag acgggtcttc
     1081 tttagccaga cggcccagag ccagacccat tttttcctgg tcagctttgg ttttcggttc
     1141 aactgcgatg gagattaccg gctcagggaa ttccatacgt tccagaatga tcggcgcatc
     1201 cgggtcacac agggtgtcac cagtggttac gtctttcaga ccgatagcag cagcgatgtc
     1261 gcccgcgcga acttctttga tctcttcacg tttgttagcg tgcatctgaa cgatacgacc
     1321 gaaacgctca cgtgcagctt tcacggagtt cagtacggta tcaccagagt taaccacacc
     1381 ggagtaaaca cggaagaagg tcaggttacc aacaaacggg tcggtagcga ttttgaacgc
     1441 cagtgcagag aacggctcgt catcacttgc gtgacgctca gccggagtgt ctttaccgtc
     1501 gtccaggata ccgttgatcg caggtacgtc aaccggggat ggcaggtaat caattaccgc
     1561 atccagcatc gcctgaacac ctttgttctt gaacgcagaa ccacaggtta ccaggatgat
     1621 ttcgttgttc agaacgcgct gacgcagagc acctttgatt tctgcttcag tcagttcttc
     1681 accacccagg tatttttcca tcagctcttc agaagcttca gctgcggatt cgatcaggtt
     1741 ctggtgccat tcgttagcca gttcaaccat gtctgccggg atatcttcgt attcgaaggt
     1801 tacgccctgg tcagcgtcgt tccagttgat agctttcatt ttcaccaggt caacaacacc
     1861 ggtgaaatgt tcttcagcac caatcgccag ctgcagcgga accgggttcg cgcccagacg
     1921 ggttttgatc tggttaacaa ctttcaggaa gttcgcaccc atgcggtcca ttttgttaac
     1981 gaacgcaatg cgcggaactt tatatttgtt tgcctgacgc catacggttt cagactgcgg
     2041 ctgaacacca ccaactgcgc agtaaaccat taccgcacca tcgagaacac gcatggaacg
     2101 ttctacttcg attgtgaagt caacgtgccc cggggtgtcg atgatgttga tgcgatgcgg
     2161 ctcatactgc ttagccatac cagaccagaa tgcagtagtc gcagcggaag tgatggtaat
     2221 accacgttcc tgctcctgct ccatccagtc catggttgca gcgccgtcat gaacttcacc
     2281 gattttatgg tttacaccgg tgtagaacag aatacgttcg gtagtagtgg ttttaccggc
     2341 gtcgatgtgc gcactgatac cgatgttacg gtagcgtgcg atgggtgttg tacgagccat
     2401 ttgtttcctc gtttatcttt taggcgttca atttaagtag cccaaagcgg gctgcttact
     2461 ggaagcgccc gcctggtgac taaaactccg aagggattac caacggtagt gtgcgaacgc
     2521 cttgttggct tcggccatac ggtgaacgtc ttcacgtttc ttaactgcag tacctttgtt
     2581 ttctgcagca tcagaaagtt cgttcgccag gcgcagagcc atggatttat caccgcgttt
     2641 acgagcagct tcaacgatcc aacgcattgc cagagcatta cgacgaaccg gacggacttc
     2701 aactggtacc tgataagtag aaccaccaac gcggcgagac ttaacttcta cagtcgggcg
     2761 cacgttttcg agagctactt cgaatgcttc cagttcagat ttaccagagc gctgagccag
     2821 ggtctccagc gcgctgtata cgatagattc agcagtagat tttttaccat ctaccatcag
     2881 gatatttaca aatttagcca gcagttctga tccgaacttc ggatccggca gaattttacg
     2941 ctgaccaatg acgcgacgac gtggcatgga aatactccgt tgttaattca ggattgtcca
     3001 aaactctacg agtttagttt gacatttaag ttaaaacgtt tggccttact taacggagaa
     3061 ccattaagcc ttaggacgct tcacgccata cttggaacga gcctgcttac ggtctttaac
     3121 gccggagcag tcaagcgcac cacgtacggt gtggtaacga acacccggga ggtctttaac
     3181 acgaccgcca cggatcagga tcacggagtg ctcctgcagg ttgtgacctt caccaccgat
     3241 gtaggaagtc acttcgaaac cgttagtcag acgaacacgg catactttac gcagcgcgga
     3301 gttcggtttt ttaggagtgg tagtatatac acgagtacat acgccacgtt tttgcgggca
     3361 tgcttccagc gcaggcacgt tgcttttcgc aactttgcga gcacgtggtt tgcgtaccag
     3421 ctggttaact gttgccatta aatagctcct ggttttagct tttgcttcgt aaacacgtaa
     3481 taaaacgtcc tcacacaata tgaggacgcc gaattttagg gcgatgccga aaagatgtca
     3541 agaaatatac aacgatcccg ccatcaccag gccatctggc tggagtgctt aaccgtaagt
     3601 ctgacgaaat cagtatagtc aatgggaatg atgtcgttcg aaatttgacc agtcaaaccg
     3661 cgggcaataa ggtcttcgtt cagggcatag accttaatgg gggcattacg cagactttca
     3721 aggtagcggt taccgtcaac tgcggcagtt acgccatctt gcaataatag cagttcgtcg
     3781 ccttcactga gcagacgcag cagcgcagca aaatccgcca gccagggtga acgatgtaat
     3841 gtgtgcagca taaaagcctc aaaacctcaa aataacatcg tagttggcga gttcgcggcg
     3901 taaggcatct gcttcgagcg gcgtggcttc gacaacaaag ggggtctgcg gatctaaccc
     3961 gcgttcgcgc agtgaagccg cacaaaccca gcactgttca atgtcgtaca gacccaacaa
     4021 tttaaaagtg gcaatgtaat cacgcgccag cactgcatcg ggcttttgtc ctggcagcag
     4081 ctgaaaaacg ccatcagcta taaagaagac agccagatca tcagttaatg cggaagttgc
     4141 cagtaaagca tctaaacctt cccgaccagc agctgtacca tgaggcgcag tagaaaaaac
     4201 aaacgcaatt cgtttcatca gaactgtacc accctgtcac aggtcagcga ggcttccgcc
     4261 agcgcgccaa gtccgcttaa ggtaaatccc ggctgaaggt ttgacgaggc cagccccagc
     4321 cttccggcct ccgtttcatc aacaacgcca cggcgtaatg ctgccgctac gcagatattc
     4381 agcgccacac catgttgcgc attcaactgt tgccagccgc gcacaaggtc aaattcgtcg
     4441 cttgccggtg aggtcaattg gttagcgtta tagacccctt cccgataaaa aaagacgctg
     4501 cttaactcat ggccttctac tatcagcgcc tgcgcaaact gaaaagcact actcgcctgt
     4561 tgcgtaccgt atgctggccc ggtcaccacg atggcaaaac gcattactta tcttgcccct
     4621 ggaaatcacc gcttttgaac tggcggatgt agagatagac agtgtgtttg gagatgttca
     4681 ggcggtcagc aacctggttg atcgcgtctt taatatcgaa gatccctttc tcgtagagat
     4741 tcagcacgat ctgacgattt ttggcgttat tagaaacatt gcgatcggca tttacttctt
     4801 cgatggtgaa ctccagcgtt tgggtaacca gatcttcaac agaagaggca aagttgacgc
     4861 ttgaaccgac atccggggtt tctggcggca caaaagtgct cataatctgc gagaagggaa
     4921 catcaagatt catattgatg cacagcagac caattacacg ctgttcgcgg ttacgaatcg
     4981 cgatagtcag ggacttcatt aatacgccgc ttttggcgcg agtaaagtag catttagaaa
     5041 cgctgctatc cgctcccgtc atatcgtgca gcatacgtag cgccaggtca gtaattggcg
     5101 aaccaatctt ccggcctgta tgttcaccgt tagcaatgcg aatggctgaa cattttagat
     5161 cctgcaaaga gtgcaaaacg atttcacagt gggagccaat aagcatcgct aacccgtcca
     5221 ccaccgcttc gtaggatttc agaatatcaa aatcggtctg gtcgaaagga cgttgatcca
     5281 gtaaatccaa ctcactggtt tcgttggtta aaagcgacct ggacatgaaa aaaaacactc
     5341 cttttcagga gcctgtcgtt aacttttcag ggcaggctca ttaatgatgc gggtaactaa
     5401 attaatacag cggaggttcc gctttccagc actaattata tccggcctgt aataaaaaaa
     5461 ccgccgcctg gtcaggcggc ggttcttaat gcttattttt tagcagaatc tgcggctttc
     5521 gcatcagctt ccggctttgc atcagccttc ggcgctggtt tcacatccag cagctctacg
     5581 tcaaacacca gggtagaatt cggtgggatc cccggaacac ccgctttgcc gtaagccagt
     5641 tctggtggaa taaccagttt gatcttaccg cctttcttga tgttcttcag accttctgtc
     5701 caccccggga taacaccgtc cagacggaaa gagagcggtt caccacgggt gtaagagttg
     5761 tcgaactctt taccgtcgat cagcgtacct ttgtagttca ctacaacagt atcgctgtct
     5821 ttcggtgctt cgcctttacc ggcttctact acctgataaa ccagaccagt tgaagaggtt
     5881 ttcacacctt tctctttggc aaatttctcg cggtactctt taccttttgc ttcgttatca
     5941 gccgcgtctt tttccatctt cgcctgagca gaagacttca cgcgagcttc gaatgcttgc
     6001 agagtctgtt cgatctcttg gtcggagagt ttgctcttat cagcaaatgc atcctgaaca
     6061 ccagcgatca gctgatcttt atccagtttg atgcccagtt tttcttgttc tttaagagag
     6121 ttttccatgt aacgacccag cgaagcaccc agtgcataag ctgatttctg atcgtcattt
     6181 ttgaacgctg ctttgctgtc agcagttgta gcaggttttg cagcttcagc agcaaaagtg
     6241 attggtgcat gcagggcaac ggccattgtg gtcgccagca gcgttacttt aaacagtgat
     6301 ttcatccata tctccaggat cggggcatct caccccaggg ttaactatta tcagaagggt
     6361 actataaagc gttgtcgaac aaatctacat acagacacgc cctattatca tctattttca
     6421 gactcttttt gtttaaatta gtttcgatga ccgcgaaatg agtgctgtct cgggcagcaa
     6481 agttaagtag aatccgcggc gaccattcga caaaagaggt gaatcatgca ggatttatca
     6541 ttggaagcac gcctggcaga gctggagagc cgactggctt ttcaggaaat caccattgaa
     6601 gaactgaacg tcacggtgac cgctcatgaa atggagatgg cgaaactgcg cgatcatctg
     6661 cgtctgctga ccgagaagtt aaaagccagc cagccgtcga acatcgcgtc gcaggctgaa
     6721 gaaacgccac cgccacatta ttgaggcgta aaaaaagcgg ggattccccg cttttttgtc
     6781 accttttcgg tattagtggc aaccacaacc gccgttgcct ttaccgccac agcagccttc
     6841 gccaccgtgt tcatgaccgt gatcgtggcc atgaccgccg cagcaaccgt cgtggtcgtg
     6901 atcgtggtga tgatcgtgcg cgccgtgaac gtgaccatga gccagttctt cttcggtcgc
     6961 ttcacgaatc gccacaacct caacgttgaa tttcaggttc tgaccggcca gcatgtggtt
     7021 accatcaacc actacgtgat cgtcttcaac cgcagtgatt tcaaccggta ccggaccctg
     7081 gtcggtttca gccaggaaac gcatacctac ctgcagttca tcaacgccca taaatacgtc
     7141 tttaggaaca cgttgcacca ggttttcgtc gtactgaccg taagcgtcgt tcgcgccaac
     7201 agcgacatca aatttgtcgc caacttcatg accttccagc gccgtttcca ggccagagat
     7261 cagggaaccg tgaccatgca ggtagtccag cggcgcactc accggagact catcaaccaa
     7321 cacaccgtct tctgtacgta cctgataggc caggctgacc accaggtctt ttgctacttt
     7381 catgatatct cctgagcatg ggaagaatag tggcgcagat tgtagcggaa ttctgcagcc
     7441 gtgtactcac tagcttaaaa aaactcggcg catatcgcta gtccggatga aaaatcccga
     7501 tcacttgctc atctttgcga acgtgatcgc gggcttcttt gtctgcttct cgcatctgat
     7561 gtccgcactt aacgcattca acaatatcaa tattattttc gcgccacatc gccattgaat
     7621 cctgtgcctg acaggccggg cattttgcgc ccgcaataaa acgttttcgg attgccatct
     7681 ttaccctcta ctcaaattca tcccagccgt ccagctggcg tcgttcttgt tgcatttcac
     7741 gctggaaaat ctcttccagt tcgcgtcggg cttccctggc gcgagaaatt tgtacagtat
     7801 cggcatgcat tgggatgagc tctcgcagca tccgcatatc caggcggcga aaatgcagtt
     7861 gcgcccgctg cgcctgatgc ggatgcatcc caagcgtgac caaagtcttg cgccccagct
     7921 ctaacgcact ggagaatgtt tcacgggaaa actgcgtcac ccctgcctgt aataactcat
     7981 gcgcttccac acgtccgcgc gctcgcgcaa gaatatgcaa atgcggaaag tgctgctggc
     8041 atatttccac cagcttcatg gtgtcttccg gctcgttaca ggtaatgacg atagactcag
     8101 cggcctctgc acccgcagaa cgtaatagat cgacctgcgt ggcgtcgccg taataaactt
     8161 tgtagccata tttacgcatc aggttaacgg cgctgatatc ccgctccagc acggtaatgc
     8221 gcattttatt tgccatcagc aaacgaccaa tcacctgacc aaaacgcccg aagcccacga
     8281 caatgacctg gggtttatca tcgttgaccc acggtttttc gtcttcttct tccggtccgt
     8341 taaactggcg ggatagccat ttatccacca gcttcatcag caacggcgtg gtcatcatgg
     8401 aaagcgtcac cgtcaccagc aacagcgcca tctggtcgcc ctggaataaa cgttgtgaag
     8461 aagcggtaga aaagaggaca aaggcaaact caccgccctg actcaacacg ccagcaaact
     8521 gcatccgctc tgagctacgc acgccataca atcgcgccag cagatacagc acgagaattt
     8581 tcaccgccac cagcacaacc acgcttatca ctacccacaa cagatgggta taaagcaccc
     8641 cgaggttgag cgacatgccg acagagataa agaacaaacc gagcagcaag cctttgaagg
     8701 gatcgatagc cgtttccagt tcatggcgat attcactttc cgccagcagc acgcccgcaa
     8761 taaatgtacc gagcgccatc gacagcccca gcgcatccat aaacaatgcg gaacccaaca
     8821 ccagcagcag cgtcgcggcg gtgaacactt cccgcacgcc agaagctgca ataaagcgga
     8881 ataccggacg cagtaaatag cgcccaccaa tcagcatgcc gacaaacgcc agcaccttca
     8941 tgccgatctt catccagtcg aaatgttcgt ctgccgaccc cgccaacaac ggcactaacg
     9001 ccagtgctgg gatcactgcc agatcctgaa acagcagaac cgaaaacccg agctggccgg
     9061 attcgctgcg attcatccct ttctcacgca tcaattgcaa cgccattgca gttgaagaca
     9121 tcgcaaggcc aatgccaccg accaccgccg cctgccaggc gaaatccgtc agcatcaata
     9181 atcccgccag caacgccgcg cttaacagca cctgtgccgc gcctacgcca aaaatcgaac
     9241 gccgcagttg ccaaagtttg gagggattca actcaaggcc gatgataaac atcaggaata
     9301 ccacgccgag ttccgaaaag tggaggatct catcgacgtc gctaataaac cccagccccc
     9361 acgggccaat tgcaatccct gccagcaaat atcccaacac agcgccaatc cccagccgcg
     9421 atgccaacgg caccgcagcc accgccgcga agagaaacag cactcctgcg agtaaaaaat
     9481 cggaaccttc catcagcggc ctcctggaga cagcggattt gccagccagt caccgtaggc
     9541 tctggcgtgg ctcgccagct cctttgcgct ttgccgtctc gcccagtaaa taatgatggg
     9601 acttaaccaa tgcatccggc acatgcccgc cgccagttca aaggggcgca gcacatcgct
     9661 catcgggtag cgattcagcg cgtcataacg gtaagcactt tccggctcgc cggtggtaat
     9721 cacgttacgc cagtactttc ccgccagttg gtttcctccc ggcccgctgg caaaaccacg
     9781 acttaatacc cggtccagcc actctttcag tagcgccggg cagctatagg tataaagagg
     9841 atgctgaaag acaatcacct cgtgctcgcg cagtaatgcc tgctcacggg ggatatcaat
     9901 aaaaaaatcg ggatagtgcg cgtaaaggtc gtgcacggta acattgctga gctgcgtggc
     9961 cggtttaagc agtacccggt ttgccaccga gtcctgagat tccggatggg catacagcag
    10021 caaaactttc gctggctgag acatcatccc cctcccggta tggtttttgt gtatagtcgc
    10081 tgttttgggc taccattgcg cccggtgcgg cagctcgccc atacattaca ttatcataat
    10141 gataagttaa catagtctga acatacggcg ccttatgatt gttttctcct cgttacaaat
    10201 tcgtcgcggc gtgcgcgtcc tgctggataa tgccaccgcc accatcaacc ccgggcagaa
    10261 agtcggcctg gtgggtaaaa acggctgtgg taaatctacc ctgctggcat tgctgaaaaa
    10321 tgaaatcagc gccgacggcg gcagctacac ctttccggga agctggcaac tggcgtgggt
    10381 gaatcaggaa acgccagcgt taccgcaagc ggcgctggaa tatgtcattg acggcgaccg
    10441 tgaatatcgt caactggaag cgcagctgca cgacgccaac gaacgtaacg acgggcacgc
    10501 cattgcgacc attcatggca agctggatgc tattgacgca tggagtattc gctcccgtgc
    10561 cgccagcctg ctgcacggcc tcggtttcag caatgaacaa ctggagcgcc cggtaagtga
    10621 tttctccggt ggctggcgta tgcgtcttaa ccttgcccag gcgctgattt gccgttcaga
    10681 cttgctgctg ctcgacgaac cgactaacca cctcgatctc gatgccgtta tctggctgga
    10741 aaaatggttg aagagctatc agggcacgct gatcctgatc tctcacgacc gcgacttcct
    10801 cgatccgatc gttgataaaa ttattcatat cgaacaacaa agcatgttcg agtacaccgg
    10861 caactacagt tcgtttgaag tacagcgcgc cacccgtctg gcgcagcaac aagcgatgta
    10921 tgaaagccag caggaacgcg tggcacatct gcaaagttat atcgaccgtt tccgtgccaa
    10981 agccaccaaa gcgaagcagg cccagagccg cattaagatg cttgagcgta tggagctgat
    11041 tgcccccgcg cacgtcgaca acccgttccg ctttagcttc cgcgcgccgg aaagcctgcc
    11101 aaatccgtta ctgaagatgg aaaaagtcag cgcgggctat ggcgatcgca ttattctcga
    11161 ctcgattaaa ctgaacctgg tgcccggctc gcgcattggt ctgttaggcc gcaacggcgc
    11221 gggtaaatcg acattaatca aactgttagc cggtgaactt gcgccagtca gcggtgaaat
    11281 tggtctggcg aaagggatca agctcggcta cttcgcccag catcaacttg aatacctgcg
    11341 cgccgacgaa tcacctattc aacatctggc acgtttagcg ccgcaggagc tggaacaaaa
    11401 actgcgtgac tacctcggcg gctttggttt ccagggcgat aaagtaaccg aagaaacgcg
    11461 ccgcttctcc ggtggggaaa aagcccgcct ggtgctggca ttaattgtct ggcagcgtcc
    11521 gaatctgctg ctgctcgacg aaccgactaa ccaccttgac ctcgacatgc gtcaggcact
    11581 caccgaagca ttaatcgagt ttgaaggcgc gctggttgtc gtttcgcacg accgtcattt
    11641 gctgcgttcc accactgacg atctctacct ggttcacgat cgtaaagtcg aaccgttcga
    11701 cggcgatctg gaagattatc aacagtggtt gagcgacgta caaaagcagg aaaaccagac
    11761 cgacgaagcg ccaaaagaga acgcgaacag cgcccaggca cgtaaagatc agaagcgtcg
    11821 ggaagcggag ctgcgtgcgc aaacccagcc gctgcgtaaa gagattgccc gtctggaaaa
    11881 agagatggag aagctgaacg cgcaactggc gcaggcggaa gagaaactcg gagacagcga
    11941 actgtatgac cagagccgta aagcggagtt gaccgcctgc ctgcaacagc aagccagcgc
    12001 caaatccggc ctggaagagt gcgaaatggc gtggctggaa gcccaggagc agcttgagca
    12061 gatgctgctg gaaggccaaa gcaactgatg gcgcagataa cgacgaccga tgccaatgaa
    12121 ttcagcagca gtgctgaatt cacccctatg cgcggcttta gcaattgtca tctacaaact
    12181 atgctgccgc gtttgtttcg tcgtcaggtg aaattcaccc cgtactggca gaggctggag
    12241 ttgcccgacg gcgattttgt cgatctcgcg tggagtgaag cccctgcaca ggctcggcat
    12301 aaaccgcgtt tagtggtatt tcacgggctg gaaggcagtc tcaatagccc ttacgcccac
    12361 ggtctggttg aggcggcgca aaagcgcggc tggctgggcg tggtgatgca ttttcgcgga
    12421 tgcagcggtg aaccaaaccg tatgcaccgc atttaccatt cgggcgaaac cgaagacgcc
    12481 agttggtttt tacgctggct gcaacgcgaa tttggtcatg cgccaacggc tgccgtcggc
    12541 tattcgctcg gcggtaatat gctggcctgt ttgctggcaa aggaaggcaa tgatctcccg
    12601 gttgatgcgg cggtgattgt ctctgcgccg tttatgctgg aagcctgtag ctatcatatg
    12661 gaaaagggct tttcccgcgt ttatcagcgt tacttgctga acctgttaaa agccaatgcc
    12721 gcgcgcaagc tggcagccta ccccggaacg ctgccgatta atctcgcgca gttaaaatcg
    12781 gtacgtcgca tccgtgaatt tgacgatcta atcaccgcca gaattcacgg ctacgccgac
    12841 gctatcgact attatcgtca gtgtagcgcc atgccgatgc tgaaccggat cgccaaaccg
    12901 acgctgatta ttcacgccaa agacgatccg tttatggatc atcaggtgat cccgaaaccg
    12961 gaaagtctcc ccccgcaggt ggagtatcaa ctgactgaac atggcggtca tgttggcttt
    13021 attggcggta cattacttca tccgcaaatg tggctggagt cacgcattcc tgactggtta
    13081 acaacgtatc tggaggcgaa atcatgttga ttccgtggca agacctctcc cccgaaacgc
    13141 tggaaaattt aattgaaagc tttgtgttac gtgaaggcac cgattatggt gaacatgagc
    13201 gtacacttga acagaaagtc gccgacgtca aacgccagct acagtgcgga gaagcggtgc
    13261 tggtatggtc ggagctgcac gaaacggtca atatcatgcc gcgcagtcag tttcgcgaat
    13321 agcctgcaac cgtcggcgga cgagataata atgatagcta agtcatggag ttactatgtc
    13381 tgccaaacat ccggtcattg cggtaacagg atccagcggc gcggggacca ccaccaccag
    13441 cctcgcgttt cgtaaaatat tcgcgcagtt aaatctgcat gcagctgagg tggaaggcga
    13501 cagttttcac cgttacaccc gcccggaaat ggacatggcg atccgcaaag cgcgcgacgc
    13561 cgggcggcat atcagctact tcggccccga ggctaacgac ttcggcctgc tggaacaaac
    13621 cttcattgaa tacggtcaga gcggcaaagg gaaatctcgc aaatatctgc atacctacga
    13681 cgaagccgta ccgtggaatc aggtaccggg gacattcacc ccctggcaac ctttaccgga
    13741 acccactgat gtactgtttt atgaaggttt acacggcggc gtagtcacgc cacagcataa
    13801 cgttgcgcag catgtggact tactggtcgg cgtggtgcct atcgttaacc ttgagtggat
    13861 tcaaaaactg atccgcgaca ccagcgagcg cgggcactca cgagaagcag tgatggactc
    13921 agtagtgcgt tcaatggaag actatatcaa ctacatcaca ccgcagtttt cccgcaccca
    13981 tcttaacttc cagcgcgttc ccaccgtcga cacttcaaac ccgttcgcgg caaaaggtat
    14041 cccgtcgctc gatgaaagct ttgtggtgat ccattttcgt aatctggaag ggatcgattt
    14101 cccctggctg ctggcgatgt tgcaaggctc attcatttcc cacatcaata cgttagtggt
    14161 accgggcggc aaaatgggtc tggcaatgga attaattatg ctgccgctgg tgcaacgatt
    14221 gatggaagga aagaaaatcg agtaactctg ctattacgcc ggataaaata ctatccggct
    14281 tcacaacggg atagttaaat cacgcggcaa ccacttcata cgagtgagta atattcaccg
    14341 ctttttccag catcaacgcc actgaacaat atttctcagc agagagatca accgcacgcg
    14401 caaccgctgc gtctttcagg tcgcgaccgg tgacgataaa atgcagatta atgtgcgtaa
    14461 acaggcgtgg tgcctcttcg cggcgttcag aggtcaattt tacttcacaa tcgaccacat
    14521 cctgacgccc tttttgcagg atagaaacca catcgatggc actgcaacca cccgccgcca
    14581 tcaacaccat ttccatcgga cttggtgctt tatcgcctga gttgccgtcc attaaaatct
    14641 ggtgaccaga ggcggattcg cccagaaaag ttaacccttc gacccacttc actcgcgctt
    14701 gcatttttgc tactccactg cgtcaatttt cctgacagag tacgcgtact aaccaaatcg
    14761 cgcaacggaa ggcaacctgg gtcatgctga agcgagacac caggagacac aaagcgaaag
    14821 ctatgctaaa acagtcagga tgctacagta atacattgat gtactgcatg tatgcaaagg
    14881 acgtcacatt accgtgcagt acagttgata gccccttccc aggtagcggg aagcatattt
    14941 cggcaatcca gagacagcgg cgttatctgg ctctggagaa agcttataac agaggataac
    15001 cgcgcatggt gcttggcaaa ccgcaaacag acccgactct cgaatggttc ttgtctcatt
    15061 gccacattca taagtaccca tccaagagca cgcttattca ccagggtgaa aaagcggaaa
    15121 cgctgtacta catcgttaaa ggctctgtgg cagtgctgat caaagacgaa gagggtaaag
    15181 aaatgatcct ctcctatctg aatcagggtg attttattgg cgaactgggc ctgtttgaag
    15241 agggccagga acgtagcgca tgggtacgtg cgaaaaccgc ctgtgaagtg gctgaaattt
    15301 cgtacaaaaa atttcgccaa ttgattcagg taaacccgga cattctgatg cgtctgtctg
    15361 cacagatggc gcgtcgtctg caagtcactt cagagaaagt gggcaacctg gcgttcctcg
    15421 acgtgacggg ccgcattgca cagactctgc tgaatctggc aaaacaacca gacgctatga
    15481 ctcacccgga cggtatgcaa atcaaaatta cccgtcagga aatcggtcag attgtcggct
    15541 gttctcgtga aaccgtggga cgcattctga agatgctgga agatcagaac ctgatctccg
    15601 cacacggtaa aaccatcgtc gtttacggca ctcgttaatc ccgtcggagt ggcgcgttac
    15661 ctggtagcgc gccattttgt ttcccccgat gtggcgcaga ctgatttatc accccgatat
    15721 caactatgca cttcgacaaa cgctggtgct atgtttgccc gtggccgttg ggttaatgct
    15781 tggcgaatta cgattcggtc tgctcttctc cctcgttcct gcctgttgca atattgcggg
    15841 ccttgatacg cctcataaac gttttttcaa acgcttaatc attggtgcgt cgctgtttgc
    15901 cacctgtagc ttgctgacac agctactact ggcaaaagat gttcccctgc cctttttgct
    15961 gaccggatta acgctggtac ttggcgtcac tgctgagctg gggccattgc acgcaaaatt
    16021 gcttcctgca tcgctgctcg ccgccatttt taccctcagt ctggcgggat acatgccggt
    16081 ctgggaaccg ttgctcatct atgcgttggg cactctctgg tacggattgt ttaactggtt
    16141 ttggttctgg atctggcgcg aacaaccgct gcgcgagtca ctaagtctgc tgtaccgcga
    16201 actggcagat tattgtgaag ccaaatacag cctgcttacc cagcacaccg accctgaaaa
    16261 agcgctgccg ccgctgctgg tgcgccagca aaaagcggtc gatctaatta cccagtgcta
    16321 tcagcaaatg catatgcttt ccgcgcaaaa taatactgat tacaagcgga tgctgcgtat
    16381 tttccaggag gcgctggatt tacaggaaca tatttcggtc agtttgcatc agccggaaga
    16441 ggtgcaaaag ctggtcgagc gtagccatgc ggaagaagtt atccgctgga atgcgcaaac
    16501 cgtcgccgct cgcctgcgcg tgctggctga tgacattctt taccatcgcc tgccaacgcg
    16561 ttttacgatg gaaaagcaaa ttggcgcact ggaaaaaatc gcccgccagc atccggataa
    16621 tccggttggg caattctgct actggcattt cagccgcatc gcccgcgtgc tgcgcaccca
    16681 aaaaccgctc tatgcccgtg acttactggc cgataaacag cggcgaatgc cgttacttcc
    16741 ggcgctgaaa agttatctgt cactaaagtc tccggcgcta cgcaatgccg gacgactcag
    16801 tgtgatgtta agcgttgcca gcctgatggg caccgcgctg catctgccga agtcgtactg
    16861 gatcctgatg acggtattgc tggtgacaca aaatggctat ggcgcaaccc gtctgaggat
    16921 tgtgaatcgc tccgtgggaa ccgtggtcgg gttaatcatt gcgggcgtgg cgctgcactt
    16981 taaaattccc gaaggttaca ccctgacgtt gatgctgatt accaccctcg ccagctacct
    17041 gatattgcgc aaaaactacg gctgggcgac ggtcggtttt actattaccg cagtgtatac
    17101 cctgcaacta ttgtggttga acggcgagca atacatcctt ccgcgtctta tcgataccat
    17161 tattggttgt ttaattgctt tcggcggtac tgtctggctg tggccgcagt ggcagagcgg
    17221 gttattgcgt aaaaacgccc atgacgcttt agaagcctat caggaagcga ttcgcttgat
    17281 tcttagcgag gatccgcaac ctacgccact ggcctggcag cgaatgcggg taaatcaggc
    17341 acataacact ctgtataact cattgaatca ggcgatgcag gaaccggcgt ttaacagcca
    17401 ttatctggca gatatgaaac tgtgggtaac gcacagccag tttattgttg agcatattaa
    17461 tgccatgacc acgctggcgc gggaacaccg ggcattgcca cctgaactgg cacaagagta
    17521 tttacagtct tgtgaaatcg ccattcagcg ttgtcagcag cgactggaat atgacgaacc
    17581 ggggagttct ggcgatgcca atatcatgga ttcgccggag atgcagccgc acgaaggcgc
    17641 ggcaggtacg ctggagcagc atttacagcg ggttattggt catctgaaca ccatgcacac
    17701 catttcgtcg atggcatggc gtcagcgacc gcatcacggg atttggctga gtcgcaagtt
    17761 gcgggattcg aaggcgtaat gcaggctaaa ccatcggata aggcattcac gccgcatccg
    17821 acatcttttg cctgatgctt cgtatttacg ccccaaccac cttcgccacc gcgtgggcga
    17881 aacgttgcat cccttcatcg atatccgcat cttccaccac cagcgacggt gcaaaacgca
    17941 tcacatccgg tccggcattc agcaccatta cgccagcctc tgcgcccgca tacaggaaat
    18001 cacgcgcccg acctttgtac tgtggtttca gctctgcgcc aatcaacagc cccataccgc
    18061 gaatatcgct aaacacatcg tactgctgat cgatcttctg cagatggtca acaaaacgct
    18121 ggcgtttcgc ctgaatgcct tccagcactt cagggggatt gatgatatca aacgccgccc
    18181 ccgctactgc acaggccaga ggattaccgc cgtaggtgga accgtgagaa ccaggatgaa
    18241 acgcagaagc aatttccgcc gtggtcagca tggcgctaat cgggaagccg ccgcctaacg
    18301 ctttcgcaga ggtcagaata tccggcgtca cgccgtagtg catgtaagca aacaaatcgc
    18361 cggtccgccc catcccgcac tgcacttcat caaacaccaa taatgcctga tgttgatcgc
    18421 acagctcgcg caagccctgc aaaaactctg gcgtcgcagc cgtcacgccg ccctcgccct
    18481 ggatcggctc aaccaccacc gcacaggtgt gatcatccat cactgctttc actgcgtgga
    18541 gatcgttaaa gggaacgtgg atgatgtctg ccggtttcgg cccaaagccg tcggaatatt
    18601 ttggctgccc acccaccgaa acggtaaaca gcgagcgacc atgaaaagcg ttatggaagg
    18661 caataatttt ggttttgaac gggctatgac gcacacaggc gtaatgacgt gccagtttaa
    18721 aggcggtttc gttagcttcc gtgccggagt tcataaacac cacgcgttcg gcaaacgttg
    18781 cctcaatcag tttacgccca agacgcagcg ccggttcatt ggtgaaaacg ttactgatat
    18841 gccacagagt ttcgccctgg gtttttaacg cgttcaccag cgcaggatgg caatggccca
    18901 acgccgtaac tgcaatgcca cccgcgaaat cgacatactc cttgccttgc tgatcccaga
    18961 ttcggctgcc ctgacctttt accggaataa actctgccgg agcataaatc ggcaggatca
    19021 cttcatcgaa agtcgcgcgt gtaattgctg tttgttcaat tgccatctca tgatcaccct
    19081 gttacgcata aacaaatgtg aaattataat cacaaaatat gcataaaaaa tcactaaatg
    19141 gcaatcagaa atcagcgatg caggaaatta gccagcagtt gatgtccttg ttcgctaaga
    19201 atactttctg gatggaactg cacaccttcc agatcccact ggcgatggcg aatccccata
    19261 atctcgcggg tttcgctcca ggccgtcacg tcaaagcacg ctggtaatga gtcaggttcc
    19321 accaccagcg aatggtagcg tgtcacggta agtggatttg ccagcccccg aaatacgccc
    19381 tcaccgctat gtgtaatcgg cgaggttttg ccgtgcatga cctttgcggc gcgcacaact
    19441 ttgccgccaa atgcctgcgc cattgcctga tgaccgaggc agacgccaag aatcggcaag
    19501 cgcccggcat agtgacgaat aacgtcaagg gagatcccgg cttcatctgg cgtacagggg
    19561 ccaggtgaga tgacaatttt ttgtggttta agggcgtcga tatccgccag cgtcaacgca
    19621 tcgttgcgct taaccagcac atccgccccc agttcacaaa agtactggta gaggttccag
    19681 gtaaaagaat cgtagttatc tataagcagg atcatggcgg ctccggtacg aaagaaccgc
    19741 gctattttac tcagattccc cggcttcgct taccactttg ctaaatatca tctgcagtgc
    19801 ggtgagatcc cccattgccc cactctgatt tgcctgattc caggcctctt tttcgatacc
    19861 ctgccagctc agttgatatc cggcatgaat cgccagttgc tcgaagaaga tccgctgtgc
    19921 cagaccactt cccacccgga agggatgcag cacgttgatt tcacaatagt aatgcgccag
    19981 ccgctcgacg aacttcgctt tctccaggcc aaccagataa ccttcttcct ccagatcctg
    20041 catcagggca ttgccctctt tttcgatata agcaaagtgg cagaacggcg tatcgccctg
    20101 ataaatatca acttcacgca gttgccctgc ccagtcgaaa atatcctggt acagctggcg
    20161 atggatagtg cgcaaatgcg gtaaaccgcg caccagcgga ccaagctcaa tggtcgcagc
    20221 acgcagcgcc gtcatttcgt aagcggcctg ttccagccgc tgctgctgac ggatgttcag
    20281 ccggttacgc atgatatcaa ggccaggata aagatacgga tcgcgccctt cgccgaattt
    20341 atcgctcata gtgcctcctc agctcttcaa ggcgcgctaa agcctctgcg gcagtaagag
    20401 tgactaaagg catctcgacg ccttcaagac ggcgactggc ctggaaatta cgattacgct
    20461 gaagctccca gagacgggac ttttgcttat cggtgagttt cttcacttga tgcctccctg
    20521 aacgtgtcca tttgccacaa gtataagcgg caaatccggg ttacgccggg agagcagaag
    20581 cgcgggcaag attgcccgcg cgagaaatca ttacggcagg actttagcgg aaaggataac
    20641 taccggtttt gacggcacat tctggtacgg accaacatca tgagtcggca cctgggaaat
    20701 cttatcggca acgtccatgc ctttcaccac tttaccaaat accgcgtaac cgaaatcacg
    20761 ctgaccatgg tcaaggaagg cgttatcggc aacgttgata aagaactggc tggtggcgct
    20821 gtctttgtca gcggtacgcg ccatcgcgat ggtgccacgc gtgttgcgca ggccgttatc
    20881 ggcttcattt ttgattggcg ggtttggttt tttctgctgc atctgctcgg tgaaaccgcc
    20941 gccctgaatc ataaagccag gaatgacgcg gtgaaaggta gtgttgttat aaaaaccgct
    21001 gttcacataa tcgacaaagt tttgcacaga cactggcgct ttttgtttat ccagctccag
    21061 ttcgatgtta ccagctgagg ttgtcaacaa tacgtgcggg tcccctttcg ctgccattgc
    21121 tgcgggagaa agagcagaaa gagcgaaaac agcagccatc gccgccaggg tcgatttgaa
    21181 catgagattt ccttaacaaa cagagcagaa aaaaagcaag tgaagagatt ctaaatagcc
    21241 ttcaagaaca aggccatccc tttacctaat tttacgtatc tgaaatatat gtaacctgac
    21301 gatcactttt tattccgtga tcaaaatcac ctcttaaaat gcaatttagc aaccgattgc
    21361 aataaaacat ttaaacagat cacaaaatca cctaaaatcg cccgctcgcg gcatatcccg
    21421 ctatgccatt ttatttttga acgcaggcca atcatgacta acagcaatcg catcaagctc
    21481 acatggatta gctttctctc ctacgcactg actggtgcgt tggttattgt caccgggatg
    21541 gtgatgggaa atatcgccga ttatttcaat ctgcctgttt ccagtatgag taacaccttc
    21601 accttcctca acgccggcat tttaatctct atcttcctca acgcctggct gatggaaatc
    21661 gtcccgttga aaacgcagtt acgttttggc tttctcctga tggtgctggc ggttgccggt
    21721 ttgatgttca gccacagcct ggcgctgttc tcgacggcga tgttcattct cggggtggtc
    21781 agcggcatta ctatgtcgat tggtacattc ctgataacac aaatgtatga agggcgtcag
    21841 cgcggttccc gcctgttatt tactgactcc ttcttcagta tggctgggat gattttccca
    21901 atgatcgccg cgtttctact ggcgcgcagc attgagtggt actgggttta tgcctgcatc
    21961 gggctggtgt atgtcgctat ttttattctg accttcggct gtgagttccc ggcactgggc
    22021 aaacatgcac caaaaacgga tgctccggta gagaaagaaa agtgggggat cggcgtactg
    22081 tttctctctg ttgcggcgct gtgctacatc ctcggtcagt taggttttat ctcctgggtg
    22141 cctgagtatg ccaaaggcct gggcatgagc ctgaacgacg cgggcacgct ggtgagtaac
    22201 ttctggatgt catacatggt cggcatgtgg gcgttcagct ttattcttcg cttctttgat
    22261 ttgcaacgca ttctgaccgt actggctggt ctggctgcga ttctgatgta cgtctttaac
    22321 accggaacac cagcacatat ggcgtggtca attctcgctc tgggcttctt ctccagcgcg
    22381 atctatacca ccatcatcac cctgggttca cagcagacca aagtaccgtc gccaaaactg
    22441 gttaactttg tcctgacctg cggaaccatc ggtactatgt tgacctttgt ggttaccggc
    22501 ccgattgttg aacatagcgg tccgcaggcg gcactgctta cagcaaacgg tctgtacgct
    22561 gtcgtctttg tgatgtgctt cctgttaggt ttcgtcagcc gtcaccgtca gcataacacc
    22621 ctgacctctc attaattgct aatgccggac ggcactatcg tcgtccggcc ttttcctctc
    22681 ttcccccgct acgtgcatct atttctataa acccgttcat tttgtccatt ttttgcacaa
    22741 acatgaaatg tcagacaatt ccgtgactta agaaaattta tacaaatcag caacatacca
    22801 cttaaggagt atataaaggt gaatttgatt tacatcaata agcggggttg ctgaatcgtt
    22861 aaggtaggcg gtaatagaaa agaaatcgag gcaaaaatga gcaaagtcag actcgcaatt
    22921 atcggtaacg gtatggtcgg ccatcgcttt atcgaagatc ttcttgataa atctgatgcg
    22981 gccaactttg atattaccgt tttctgtgaa gaaccgcgca tcgcttatga ccgcgtacac
    23041 ctctcgtctt acttctctca ccacaccgcc gaagagctgt cgctggtgcg cgaaggcttc
    23101 tacgagaaac acggcatcaa agttctggtc ggcgaacgcg ctatcaccat caaccgtcag
    23161 gagaaggtga ttcactccag cgccggacgt accgtttttt atgacaagct gatcatggca
    23221 accggttcct acccgtggat cccaccaatc aaaggttctg atactcagga ctgctttgtc
    23281 tatcgcacca ttgaagacct caacgccatt gaatcctgcg cccgtcgcag caaacgcggt
    23341 gccgttgttg gtggcggcct gttaggtctg gaagccgctg gcgcgctgaa aaacttaggt
    23401 attgaaaccc acgttatcga atttgcccct atgctgatgg cggaacagct tgatcagatg
    23461 ggtggcgagc agctgcgtcg caaaatcgaa agtatgggcg tgcgcgttca caccagcaaa
    23521 aacacccttg agattgtgca ggaaggtgtc gaagcgcgta aaaccatgcg ttttgccgac
    23581 ggcagcgaac tggaagtcga ctttatcgtc ttctctaccg gtatccgtcc gcgcgataag
    23641 ctggcaaccc agtgtggtct ggacgttgct ccgcgtgggg gtatcgtcat taatgattcc
    23701 tgccagactt ccgatccgga tatctacgcc atcggtgaat gcgcaagctg gaacaaccgt
    23761 gtatttggtc tggtagcacc tggctacaaa atggcgcagg tcgccgttga ccatattctc
    23821 ggtagcgaaa acgcctttga aggtgctgac cttagcgcca agctgaaact gctgggcgta
    23881 gacgtaggcg gtattggtga tgcgcacggt cgcacgcctg gcgcacgtag ctacgtttac
    23941 ctcgacgaaa gtaaagagat ctacaaacgc ctgattgtca gcgaagacaa caaaaccctg
    24001 ctcggtgcgg tactggtggg cgataccagc gactacggta acctgctgca actggtgctg
    24061 aacgctatcg aactgccgga aaacccggat tccctgatcc tgccagcaca ctctggtagc
    24121 ggcaagccgt ctatcggtgt tgataaactg ccggacagcg cgcaaatctg ctcctgcttc
    24181 gacgtcacta aaggtgatct gattgctgcc atcaacaaag gctgccacac agttgcggcg
    24241 ctgaaagctg aaaccaaagc gggtactggc tgcggtggct gtatcccgct ggtcactcag
    24301 gtactgaacg cggaactggc gaaacagggc atcgaagtta acaacaacct gtgcgaacac
    24361 tttgcttatt cgcgtcagga actgttccat ttgatccgcg ttgaaggcat taaaaccttc
    24421 gaagaactgc tggcgaaaca cggcaaaggc tacggttgtg aagtttgtaa accaaccgtc
    24481 ggttcgctgc tggcctcctg ctggaacgaa tacattctga agccggaaca tactccgctg
    24541 caggattcta acgacaactt cctcgctaac atccagaaag acggcaccta ctcggtgatc
    24601 ccgcgttctc cgggcggtga aatcaccccg gaagggctga tggcagtggg tcgtatcgcg
    24661 cgtgaattta atctctacac caaaatcacc ggctcccagc gcctggcgat gtttggcgca
    24721 cagaaagacg atctgccgga aatctggcgt cagctgattg aagctggctt cgaaaccggt
    24781 catgcctatg cgaaagcact gcgtatggcg aaaacctgcg tgggtagcac ctggtgccgc
    24841 tacggtgttg gcgatagcgt cggcctcggc gtggaactgg aaaaccgcta caaaggcatc
    24901 cgtacgccgc acaaaatgaa gttcggtgtc tccggctgta cccgtgaatg ttcagaagct
    24961 cagggtaaag acgtgggtat tatcgccact gaaaaaggct ggaacctgta tgtttgcggt
    25021 aacggcggca tgaaaccgcg tcatgcggat ctgctggcag cggatatcga tcgcgaaacg
    25081 ctgatcaaat atctcgaccg cttcatgatg ttctacatcc gtaccgccga caaactgacg
    25141 cgtaccgcgc cgtggttaga aaacctcgaa ggcggcatcg attacctgaa agcggtgatc
    25201 attgacgaca aactggggct gaacgctcat ctggaagaag agatggcgcg cctgcgtgaa
    25261 gcggtagtgt gtgagtggac tgaaacggtc aatacgccgt ctgcgcagac tcgcttcaaa
    25321 cacttcatca acagcgacaa gcgtgacccg aacgtgcaga tggtgccaga gcgcgaacag
    25381 caccgtccgg caacgccgta tgaacgtatc ccggtaactc tggtggagga caacgcatga
    25441 gccagtggaa agacatctgc aaaatcgatg acatcctgcc tgaaaccggc gtctgcgcgc
    25501 tgttaggtga cgagcaggta gcgattttcc gcccgtatca cagcgaccag gtgtttgcga
    25561 tcagcaacat tgatccgttc ttcgagtcca gcgtgctatc acgcgggctg attgcggaac
    25621 accagggcga gctgtgggtc gccagcccgc tgaaaaaaca gcgttttcgc ttaagtgatg
    25681 gcttgtgcat ggaagacgaa cagttttccg tcaaacatta cgaagcgcga gtgaaagacg
    25741 gcgtggtgca gctgcgcggt taatgtttta acgggaggcg caatgcctcc cctttttgca
    25801 tggtcctgta atgaccttcg gtatattgca gaacattttt taaacttttt gttttatttt
    25861 ttgtttttat tttttaaagg ataatcaaat gtttacagac actattaata agtgtgcggc
    25921 taatgctgcg cgcattgcac gcctgtcggc aaataacccg ctcggctttt gggtcagctc
    25981 cgccatggcg ggcgcatatg tgggtcttgg gatcatcctg attttcacgc tcggtaattt
    26041 gctcgacccg tccgtacgtc cgctggtgat gggcgcgacc tttggtatcg ccttaacgct
    26101 ggtgattatc gccggttctg aactgttcac cggacacgcc atgttcctca cctttggggt
    26161 aaaagcgggc agcatcagcc acgggcaaat gtgggcaatc ctgccgcaaa cctggctggg
    26221 taacctggtc ggttccgtct tcgttgccat gctctatagc tggggcggcg gtagcctgct
    26281 gccggtagat accagcatcg ttcactccgt cgcgctggct aaaaccactg caccggcaat
    26341 ggtactgttc ttcaaaggtg cattgtgtaa ctggctggtt tgcctggcta tctggatggc
    26401 gctgcgcact gaaggggcgg cgaaatttat cgctatctgg tggtgtctgc tggcatttat
    26461 cgcgtccggc tacgagcact ccatcgctaa catgacgctg ttcgccctct cctggttcgg
    26521 caaccacagc gaagcctaca cgctggcggg tattggtcat aacctgctgt gggtgacgct
    26581 gggtaatact ttatcgggtg ccgtattcat gggattgggt tattggtatg ctacgccgaa
    26641 agcgaatcgt ccggttgcgg acaaatttaa tcaaactgaa acggctgccg gttaattact
    26701 aaggggtttt tacgtggatc atttgcctat attttgccaa ttacgcgatc gcgactgtct
    26761 gattgtcggc ggtggtgatg tcgcggaacg caaagcaagg ttgctgttag acgcaggcgc
    26821 tcgcttaacg gtgaatgcat tagcgtttat tccacagttc accgcatggg cagatgcagg
    26881 catgttaacc ctcgtcgaag ggccatttga tgaaagcctt ctcgacacct gctggctggc
    26941 gattgcagcg acggatgatg acacgcttaa ccagcgcgtc agcgaagccg ctgaagctcg
    27001 tcgcatcttc tgtaacgtag tcgatgcgcc gaaagccgcc agctttatta tgccgtcgat
    27061 tattgaccgc tcaccgctaa tggtagcggt ctcctctggc ggcacctctc cggttctggc
    27121 gcgcctgttg cgcgaaaaac tcgaatcact gctgccgttg catctgggcc aggtggcgaa
    27181 atatgccggg caattacgcg ggcgagtgaa acaacagttc gccacgatga gtgagcgtcg
    27241 ccgtttctgg gagaaattgt tcgttaacga ccgcctggcg cagtcgctgg caaacaacga
    27301 tcagaaagcc attactgaaa cgaccgaaca gttaatcaac gaaccgctcg accatcgcgg
    27361 tgaagtggtg ctggttggtg caggtccggg cgatgccggg ctgctgacgc tgaaaggact
    27421 gcaacaaatt cagcaggcag atgtggtggt ctacgaccgt ctggtttctg acgatattat
    27481 gaatctggta cgccgcgatg ctgatcgcgt tttcgtcggc aaacgcgcgg gataccactg
    27541 tgtaccgcag gaagagatta accagatcct gctgcgggaa gcgcaaaaag gcaaacgcgt
    27601 ggtgcggctg aaaggtggcg atccgtttat ttttggccgt ggtggcgaag agctggaaac
    27661 actgtgcaac gcgggtattc cgttctcggt ggttccgggt attaccgcag cttctggttg
    27721 ctctgcctat tcgggtattc cactcacgca tcgcgattat gcccagagcg tacgcttaat
    27781 taccggacac ttaaaaaccg gtggcgagct ggactgggaa aacctggcgg cagaaaaaca
    27841 gacgctggtg ttctatatgg gattgaatca ggccgcgact attcagcaaa agctgattga
    27901 acacggtatg cctggcgaaa tgccggtggc aattgtcgaa aacggaacgg cagtcacgca
    27961 gcgcgtgatt gacggtacgc tcacgcaact cggtgaactt gctcagcaaa tgaacagtcc
    28021 atcgctaatt attattggtc gggttgttgg cctgcgcgat aaactgaact ggttctccaa
    28081 ccattaattt aacccgggcc agagaattct ggccttctta atgactcttt ttatatattc
    28141 agcaaataaa acataacccc ttaataaata ttatctgcta atgggttata ttcaccgtta
    28201 cagcgactta caaaattaag tcattcgctc actgacgcca attaaatatt cctgtatcgt
    28261 tcttaacgag cagtctgctt atattcatat aatcaatgaa tattaattaa tatataatac
    28321 atagggaatg taaatgaaca aatttattaa agttgcactg gtaggtgcag tactggctac
    28381 gttaactgca tgtactggtc atattgaaaa ccgtgataag aactgctctt acgactacct
    28441 gctgcacccg gcaatttcta tttctaaaat cattggcggt tgcggtccta ctgcacagta
    28501 agtctctgac agaaatcggc taacaccggt ttaattgccc gacgccctct ggattttccg
    28561 agggcgtatt tgtttctatg tccttcttgc ccccgcccgt aaaaatattt tcactttaaa
    28621 ttcaatttga taactacatc cattagccac agcgcgatcc tgtgcgaaat tttgtgatct
    28681 tcctccacat tacataacat catatgttgt tatattcatc atgcattgtc atgttacctt
    28741 ttaaatgact gcaaactctc ccctacaacg tattggacaa gaaaaaggta tcgctatggg
    28801 aagccaggaa ctccaacgca agctcggatt ttgggccgtt cttgcaatcg ccgtcgggac
    28861 aaccgtcggc tccggtattt ttgtatctgt gggtgaagtg gcaaaagcag cgggcacgcc
    28921 gtggcttacg gtgctcgcgt ttgtcattgg cgggttaatt gtgatcccgc aaatgtgcgt
    28981 ctatgcggaa ctatccaccg cttatccgga aaatggcgca gattatgttt atctgaaaaa
    29041 tgccggaagc cgaccgctgg ctttcctctc cggctgggcc agcttctggg ccaacgatgc
    29101 gccgtcattg tcgattatgg cgctggcgat tgtcagcaat cttggctttt taacgcctat
    29161 cgatccgttg ctcggtaaat ttatcgccgc cggattaatt atcgccttta tgttgctaca
    29221 cctgcgctcc gttgaaggcg gcgcagcgtt tcagacgcta attaccatcg ccaaaattat
    29281 cccgttcact atcgtcattg gccttgggat cttctggttt aaagcggaga attttgacgc
    29341 ccctaccacc actgcgattg gcgcaacggg cagctttatg gcgctgctgg cggggatctc
    29401 tgccaccagt tggtcgtata ccggcatggc ctctatctgt tatatgaccg gcgaaattaa
    29461 aaaccccgga aaaaccatgc cacgagcgct gattggttcc tgtctgctgg ttctggtgct
    29521 ctacaccctg ctggcgctgg tgatttccgg cctgatgccc ttcgacaaac tcgccaattc
    29581 tgaaacgccg atttccgacg ccctgacctg gatccccgca ctcggcagca ccgctgggat
    29641 ctttgttgcc atcacggcga tgatcgtcat tcttggttcg ctttccagct gcgtgatgta
    29701 ccagccgcgg ctggaatacg cgatggcgaa agacaacctg ttctttaaat gcttcggcca
    29761 tgtgcatccg aaatacaaca cgccggatgt ctccatcatc ctgcaagggg cgctggggat
    29821 cttcttcatc ttcgtttccg atctcaccag cctgctgggt tatttcaccc tggtgatgtg
    29881 tttcaaaaat accctcacct tcggctccat catctggtgt cgtaaacgcg acgattacaa
    29941 accgctgtgg cgtactccgg ctttcgggct gatgaccacc ctcgccattg cgtcaagcct
    30001 cattctggtc gcctcaacct ttgtctgggc accgattccc ggccttatct gcgccgtcat
    30061 cgttattgct actggtctgc ctgcttacgc cttctgggcg aagcgtagcc gccagctcaa
    30121 cgctttgtcg taatgttatc tggagaaaat aaaatgttgg atattgataa aagcaccgtg
    30181 gactttctgg tcaccgaaaa tatggttcag gaagtggaaa aagttctcag ccatgacgtt
    30241 ccgctggtgc acgccatcgt ggaagagatg gtgaagcgcg acattgatcg tatttatttc
    30301 gttgcctgcg gctcgccact caacgcggcg caaacggcga aacatctggc ggatcgcttt
    30361 tccgatcttc aggtctacgc catttccggc tgggagttct gcgataacac cccgtatcgc
    30421 ctcgacgatc gttgcgcggt gattggcgtt tctgactacg gtaaaacgga agaggtaatc
    30481 aaagcgctgg agctgggccg ggcctgcggc gcactcactg cggcgttcac caaacgcgcg
    30541 gacagcccga ttacctcggc ggcggaattt agcattgatt atcaggccga ctgtatctgg
    30601 gaaattcacc tgctgctctg ctacagcgtg gtgctggaga tgatcacccg cctcgcgccg
    30661 aacgcggaaa tcggcaagat caaaaacgat ctcaagccgt tgccgaatgc gctcggtcat
    30721 ctggtacgca cctgggaaga aaaaggccgc cagcttggtg aactggccag ccagtggccg
    30781 atgatttata ccgttgctgc gggtccgctg cgtccgctgg gttacaaaga aggcattgta
    30841 acgctgatgg aatttacctg gacgcacggc tgcgtgattg agagcggaga gttccgccat
    30901 ggcccgctgg agattgtcga accgggcgtt ccgttcctgt tcctgctcgg caatgatgaa
    30961 agtcgccaca ccaccgaacg cgccattaac tttgttaaac agcgtactga caacgtgatc
    31021 gtcatcgatt acgccgaaat ttcgcaaggg ctgcacccgt ggctggcacc gttcctgatg
    31081 ttcgtgccaa tggagtggct ctgctactac ctgtctattt acaaagatca caacccggat
    31141 gaacgccgct attacggtgg tctggtggaa tattaatccc tctcccggcc cggcaacggg
    31201 ccggatttac gcaaggagtt acccgatgaa aacaggtatg tttacctgcg gccaccagcg
    31261 gctgcctatt gaacacgcat ttcgtgatgc aagcgagctg ggttacgacg gcatcgaaat
    31321 ttggggcggt cgcccgcacg cgttcgcgcc ggacttaaaa gcgggcggca tcaaacaaat
    31381 caaggcgctg gcgcagacgt atcagatgcc gattatcggc tatacgccag aaaccaacgg
    31441 ctatccgtat aacatgatgc tgggcgatga acatatgcgt cgcgaaagcc tcgacatgat
    31501 caagctggcg atggatatgg caaaagagat gaacgcgggt tatacgctga tttccgcggc
    31561 ccacgcaggc tatctcacgc cacctaatgt tatctgggga cggctggcag agaacctaag
    31621 cgaactgtgt gagtacgcgg aaaacatcgg catggatcta atcctcgaac cgttaacgcc
    31681 gtatgaatcg aacgtcgtgt gtaatgctaa tgatgtgctt catgcgctgg cgctggtgcc
    31741 ttcgccgcgc ctgttcagca tggtcgacat ttgcgcgccg tatgtccagg cggaaccggt
    31801 gatgagttat ttcgacaaac tgggcgataa attatcgtca tctgcatatt gtcgacagcg
    31861 acggggccag cgacacgcat tacattcctg gagaaggcaa aatgccgctg cgggaactga
    31921 tgcgcgatat tattgagcgg ggctatgagg gttactgtac ggtggagctg gtgacgatgt
    31981 atatgaacga gcccagactc tatgcccgcc aggcgctgga acgctttcgc gcgctgctgc
    32041 cggaggatga gagatgaaaa ccctggcgac aatcggcgat aactgcgtcg atatctaccc
    32101 gcaactgaat aaagcgtttt ctggcggtaa tgcggtcaat gtggcggtgt actgcactcg
    32161 ctacggcata cagccgggat gcattacctt ggtgggtgac gatgactacg gcacaaagct
    32221 gaagcaggat ctcgcccgca tgggcgtcga tatcagccat gtccatacga aacacggcgt
    32281 taccgcacaa actcaggtgg aactgcacga caatgatcgc gtttttggcg actacaccga
    32341 aggcgtgatg gccgactttg ccctgagtga agaggattac gcctggctgg cgcagtatga
    32401 cattgtgcac gcggcaatct ggggacatgc ggaagacgca ttcccacagc tgcacgctgc
    32461 gggcaaactt accgctttcg acttctccga caagtgggac agcccgctct ggcagacact
    32521 ggtgccgcat ctcgattttg cctttgcctc cgcaccgcaa gaagacgaag cgctgcgtct
    32581 gaagatgaaa gcgattgttg cccgtggcgc aggaacagtg attgtcacgc tgggtgaaaa
    32641 cggcagcatt gcctgggatg gcgcgcagtt ctggcgtcag gctcctgaac cggtgacggt
    32701 tatcgacacc atgggtgccg gagattcgtt cattgccgga ttcctttgcg gctggtctgc
    32761 ggggatgaca ttaccgcagg cgatggcgca gggaacggcg tgcgcggcga aaaccattca
    32821 gtatcacggt gcctggtagg tataacgttg gcgtgagcat ctccgcgcca acgtgctgtt
    32881 acttgccgga aaacgaccct ataatccgtg taattcattc tttatttcag ggtcgattat
    32941 gtcagctacg gaccgctact ctcatcaact cctctacgct accgtccgcc agcggctgct
    33001 ggatgatatc gcgcaggggg tttaccaggc cgggcaacag atccctaccg aaaacgagct
    33061 ttgtacgcaa tataacgtca gccgcattac tattcgcaaa gctatcagcg acttagtggc
    33121 agacggcgta ctgatccgct ggcagggcaa aggcaccttt gtacaaagcc agaaagttga
    33181 aaacgccctg cttactgtca gtggttttac cgattttggc gtctcacaag gcaaggcgac
    33241 gaaagagaaa gtgatcgaac aggaacgggt cagcgccgcg ccgttttgcg aaaagctgaa
    33301 catccccgga aacagcgaag tgttccatct ctgccgggtg atgtatctcg ataaagagcc
    33361 gctgtttatt gatagttcat ggatcccgct gtcgcgttat cctgactttg atgagattta
    33421 cgtcgaagga agctccacct atcagttatt tcaggagcgt tttgacacgc gagtggtcag
    33481 cgacaaaaag accatcgata tctttgccgc cacccgcccg caggcaaaat ggctgaaatg
    33541 cgaactgggc gaaccgttgt ttcgcatcag caaaatcgcc tttgatcaga atgacaaacc
    33601 ggtgcacgtc tccgaactct tctgccgcgc caatcgcatc accttaacga ttgataataa
    33661 aagacattaa ccgtaggccg gataagatgc gccagcatcg catccggcga tgctggcgcg
    33721 ttgaatttta catcccgtac gttcccctca ccctaaccct ctccccaaag gggcgagggg
    33781 accgttcagt acggaattta gcttgacggt gctatccagc attaaatact ggcaatactc
    33841 tcacgcaaaa tccgcagcac cgtctcttca ccgttgcgat tcgggttaat gcgaatcgca
    33901 caatgttctg attgtggatt cgcctggcga aacgttccgg aaaggcgata aaagagcggc
    33961 gggatttcat atttcgactc tgcacccacc gggtaaggca aggcaccgcg cttttgcgcc
    34021 tcttccagca ctctggcggc aatcggctga tgaaactcga caatcaacac cttcgactgc
    34081 gcattagcaa tcaccgcgct tttcacttcc ggcaccgcac caccgttaag caaagccagc
    34141 aaccgttcag atacccccgc ctgcaccgcg tgcatcactg gcgcaaacac cagcccacac
    34201 aagacttcca gcgcctgagc gccctgaatc tggctgccgc cggaatagag cgtggagcga
    34261 atccgactaa taacatcggc atcgcctacc accgcgccaa caccctccgg cccaaacagc
    34321 ttgaagcagg aaaaagtcga gacatttgcc ccgcattcgc agccaattcg cgccactttc
    34381 atcaccgcat agttgtcatc ggttaatgca ggaacgccag ccgagcgcaa tgttgccagc
    34441 acatctgcca gaatgtagcc gtcctgcggc tgctggcgcg tatgctgcac cagcgccgca
    34501 tccggtagtt gctcgtcgac gacctgcttc agtgccgaca ggtcattgaa atcagcagta
    34561 ataagcgtca gccccatctg ctcaataatg acctgtgtcg tcgggtaaac aggcgcatca
    34621 tgtaccagaa gacgctgccc cggtttgagc aaagccgcca acgccgcacg aatcgcgcca
    34681 gtacccgcac cctgcaccag cgccgcagcc tgtgcgtgaa atgcatcagc cagcacctgt
    34741 tccacacgct gggtaacgcg cggttgattc agtcctggcg ttaagcctaa atcaccgcca
    34801 gtaagaaact cgctgccggg gaaatggcga caaatgctat ccaccagcgc aaactgcttt
    34861 tgctgcgcct caataatcgt cagactttgc agaggaaacg tcttcatagg tgccttcctt
    34921 aagccggaac gaacaggcca agccagtaaa gaacattcag cagaataccg gtgatcatta
    34981 ctgccacgac cggtgccgcc attttctgta ccggacggcc cagggattcg ttgaggaagt
    35041 aaatggcaac cgcgatagag aatccggtat aacccgccat cttaattgct gcgaaaatcg
    35101 aaccgaccag cagcgccact tccatcagca tattcatggc gttacggatg ttatccgacg
    35161 cattacgcac cgacgggtag cgtcccagcc atttgccgat cgaacgaagc agcaagactt
    35221 ccgccgaaat aaccactgcg cctaataccg ctgcaactat cggattcggc gagagatagc
    35281 ccaccgcata aacaaaggta aagcccgcaa ctgcatacac acccgttgct aaggcggtgg
    35341 tggcaatcaa cggtacaaaa cccagcccgc gcataaattc tgccagtgcc gcctggttga
    35401 tcagcgtttg cgattgttcc ggcgttacgc ctgcggagta cgctttctcc agagtgaaga
    35461 tcgacacttc actgccggcg aaaatcttca tgctggcaac ggcggcaatc aatgctccca
    35521 cgatggcgat atagggtaag tttttgataa tccgcgacgt gcgttcttca aacaccgaaa
    35581 gcccgctggc atcaatatca ttttcatcac gatgacgcag gtcgtgagtt atcgcgatcc
    35641 ccagcagcat caccatgcca ataaagattt cgatggattc agggttaaga tgtgggaaat
    35701 agcgcacgac taccacacgg gtcatcagaa caaccacggc ggcgaccaga ctttgcttcc
    35761 agccaaactg gtagaaaatc gccaccagcg ggaacagtgc aaaagcagaa accaccggcg
    35821 agcttaattc cccgaggcta cctaatacat ccaccggcag cgcggtcagc agctggttta
    35881 ctggcaacag gcaagtaagg atcaacacgc cccagatagc gccaagacca aacgccatca
    35941 ggctgtttat cgccagtacg ccgaggatat cggtaggaag aaagagtaac catgcgttga
    36001 gcagcccggt tttcagggta aaagagatcc ccaccgaggc cacaaaacca atgctcagac
    36061 caaaagcgat actccccgcc tcgcgacggt tcatatagcc ttcaatcagt tgcggcagga
    36121 tcgggcggat gccgtcatga aaaacagccg ccgagcgatg cgccagaagc gatgtcatac
    36181 ccgtcaggca cgccaccacg ataatctgaa tatacagatc catagtcgtg ccttttattt
    36241 cagatggtta atcagcatcg gaatcgcgtg ttcgacgtgc tcaacggaaa ggccaaacgc
    36301 cacttttcct tcagcgatca ttttggcgat atgctcgtct ttcgctttaa tgcctggttt
    36361 ggcaatggtg caacttttgt tatagccaat caccgcgatg gcaatcgaca atgccgcgcc
    36421 cgcaccggta ttacacgcac cgatgtagta atccagttgc ccggatttca ctttcattgc
    36481 cgcttccatg tcgttgtgaa tgaaaacttc aaaactgcca ggagccgtcg cttcaatggt
    36541 ttttttaatc tgctcacgct gtaagcctgc aacgccaatc tttttcatga gtctgtcctt
    36601 attggaaaaa ttgagaggga gtttcacgta acatcacatc cacatcggcc tgactgaatc
    36661 ctgactggcg caattgcgga ataaaggtgg tcaataaata gtcatagcca taaccaccat
    36721 tggcttttaa atgggagcgg cgcgtaatat ccatcgacag catgacgcgg ttcagcaacc
    36781 cacggtcacg tagcgcgtgg agcatcgcaa tgcgcttttc gtccgggtag taactgttct
    36841 taccaatggt gtcgaactgc acgtacgcgc cgagatcgat catcttcaaa atgttgtcga
    36901 ggttgtcttt cagatcgcag tgaccaacgg tgacgcgcga aagatcaact ccgtgggctt
    36961 gcaacagagc cagttgttcc agccccatgg tgctgaacga cgtatgcgtg gagatcgggc
    37021 gtccggtctg gttatgcgcc agcgcagcgg caataaatac cttctcttcc agcggcgtaa
    37081 tctttccttc gctggagccg atctccgcga tgatcccggc tttcagctcc gtgccatcga
    37141 taccctgttc aatttcatcg accatctcct gcgccagttc ctgcacgctg cgggtcacca
    37201 catgttccgg gaaaaacgca tcctggtaat aaccagtaca ggccaccacg ttgatcccgg
    37261 tttcgcgcat tacaccgagc ataaattgcg cattgcgccc catgtaacgg ttggtcatct
    37321 caatcacatt acgtacaccc cgggtcatca ggtcgttcat ctcctggcaa atgaacgcat
    37381 actgatcaag gcggcagtcc acgttgtttt taaagccgga gagatcaata tgcagatgct
    37441 catgggccag ggtgtaaccc gtcggatcaa aactcatagg gtgtctcctg caaaccggag
    37501 ggaggaaaga aaagagcgac cattttgtgg cggtggcgca cggaaaaatt cacacacggt
    37561 agctcccaca tcagaaagcg tggtgcgcac accgagctgc gtagcagcca ggccttgctg
    37621 ataaaccagc agcggcacca cttcgcgggt atggtggctg tgaccaatgg tcggatcgtt
    37681 gccgtgatcc gccatcacga ccaggcaatc atctggctgc atcgcatcaa caagccgggc
    37741 aaggttacgg tcaacgacct gcaaacgttc ggcataacgt gcgacgtctt ctgcatgacc
    37801 agcgaggtcg gtttcctgga tattggtgca aataaacgcc gtcggatggg tgttaaattc
    37861 gtcgagggtg atatccataa tccgctggct atccaccagg ttttgccagc tcacgccata
    37921 aggattgctg acgatatctg ccaccttacc caccagcacg gttggcacgc ccgcctcata
    37981 cagtttttgc ggcacctgca cttttttatc gacaccgtag cccatatgca cgacctggaa
    38041 gccgttgtca taagcgccag aacgcggcgc attgatacca ataaagcgcc cttctttgct
    38101 ttctgcggca tcgagaatgc gttgactgtc ggttaacagg ccaccaaatg taatgacccg
    38161 accgacctgt acctgctcac gcacgatacg accaattttg attgcgtcgt caaaagagat
    38221 cacagagaga ttggcggtaa tgttatagac ttggcctaaa tccgcctcga gattatcgcc
    38281 aatcgcaacc gcctgattga cccacagaaa ttacagttca tcgccacggc gctccacctg
    38341 ccaaccggcg gatactaatg cctgctcaac acggtcaatc acatcgcgaa aaggcatccg
    38401 cagcggcggt aacgggcgcg tgcctaaaat ttcctgatgc cccataaagg tatcgccacc
    38461 ttcatgttgc agctctgcca cgccccaggt tgcggaatct gacggctgca tatcgcctgg
    38521 cgcataaccc aatgcgttga ttagccccag cttctccagc gttggtagct gcaaatgcgg
    38581 caactggctc aggatgtgac cacatgtatt cgctcccgca tcttgcggac gcaccagcgt
    38641 gacatctttc attgccccta cgccaaagct atcaatcact aacaccacaa atcgcgccat
    38701 tacgccccca gagagtttcc aagactgtca taacgaccaa cgattttcgg ttcgccacgg
    38761 tgaataccgg acaccagcac cacatcgcta cgggtgacaa aaatctgcgt gcggaaacag
    38821 agcaccactg cactgcttac cggaaactcg cctgccagcg acagggtgta gtcgatactg
    38881 ctgtcatcca cagttttgag attggtttca gtaatctttt gattttctgg cgtaaacacc
    38941 aacgcatgtt gcgcatgacc acgacggtag taaccgccgc cgtagcagta gctgtcgcca
    39001 cggaaatgat gggagatttc actcaaccag agcatcgcga tacgttcaga ctgatcgccc
    39061 tgctggtttg ccggaatagt gcccgtcagc gcatgaccgg gttcggcatg agtcacaccg
    39121 tattacgcca gtaatggcag cgaagtgcag ctggtcgctg aaggcgcgtt cagttgctca
    39181 agtgcaatac cagatttcgc cagttgatcc cgtgcctgta tcagcgtgtg aagattcggt
    39241 gtcggcaaaa cttttccggc agcctcatcc caaagcaggc aagggaaatg ggtaagtccg
    39301 gctaaatgca gccccggcag tttcttaatt tcagcaacca cctcgtgcag cgaatgctgt
    39361 acaaaaccgc tctcctgccc cggataaaga aaatcatcgt cgctataaac tttgagcagc
    39421 acaaactgca cacgtccggc cttcaccgct gccgcagaaa cttcccgcgc tttgtcgaga
    39481 gtaaacacgg tgatgacgtc ggtgccctgt tcaacggcgt cagcaacctg atgacaaggg
    39541 atttgtacca gatgcccctg atgtgccacc gacaaaccag cgcggcgcat gactcgcgcc
    39601 tctttgtaat ccaccgccac aatgccgctg taacctaatg ccagcaattt ttccgccagc
    39661 catgggttac ggccaaactg cttggtcatc agatacagtt caatgccgta aagccgcgcc
    39721 gtctcaatca gtcgcttacc gttttccagt acctgatcca cgtcgatcac ccagctgtcc
    39781 ggggcgatct tgccctgctg ccacaaactt agtgcggcgg aaatcagcgc cgggttctgg
    39841 cgtttcaatg cttctacaaa catcttgttg cctcgtttct gttccttgaa ttaaatattc
    39901 atttttttga atatttaggt tgtgcgacgt ggatttgtcg ggagcttcaa acctcttcgt
    39961 tagcagccat ccataatcca taaaggttgg ccagcaaata gccttcttca cacgggttaa
    40021 cttccagtgc gaattccttc aacaacacct gatgcaattg cacaacggct tgccagtggc
    40081 tggattgcgc cagctctgcc agcaattcgt tatccagcgg ctctatttct tcaccacggc
    40141 gactgcgcat cagtgcactc gccatatgtg tcatcgccat cgttccttgc tcactgcgca
    40201 ccggcagatg gcactctgtt tccagtacgt tgacgacctg catcatgcct ttgcagatgt
    40261 ccttatcaat aacgcctgcc tcgcaaagca ggttgagtct ggtttccata tcagatgcct
    40321 ttcgtttaat aactcggttc ctgttcccca ctcactaccc gttgttgatg ggcgagcagg
    40381 gcgtgtttat ctacaacggc acgcagcagt tgttgcattg ggtaatcatc gactcgcgtc
    40441 aggaccacgg cttcggtggc ctgtaaaaat cgcggatcgt ctgtgagcgg cgtcgcctct
    40501 aatcccagca tggtcagttc gttttccgcc accacgttcc agataaccgc atcgacatcg
    40561 cctttaacaa tgcgttgtaa actctcgtga taagagagat cgacacgttc cacatcacta
    40621 tcgccaaaaa agacatcggt catgattttc tgatccgccg aacggttatc cagccccacg
    40681 cgcttcacgt ttgcggactc gcctttacgg caaatcaact ggtgctcgcc aacgtaggtg
    40741 tgcggcccca actccagcgc gaggcataac cctttttgcg tgagataact ttccgccgcc
    40801 agtcgggaaa ccaccgccat gtcatacacg ccgttaagta aacactccac gcgaatatcc
    40861 gcgccgcgca tatgcgcata gtaaaaagga atgccatcaa actgagcttt cagcccgctc
    40921 gctaaacctt cgtacaagcg ggtatagggc aaaggcatcg cacataccac gttgttgata
    40981 tccacatgac tcagcaatgc tttgttatcc atctcgacca gataactgcc attgcgccca
    41041 cggcgttcaa tccgtatcgc tccgcttgat tccaacgttt tcaacgcggc ctgcgtcagg
    41101 ccaaccgatg aacggcattc agttgccagc tcatctatgg ttttcagtcg attaccgcat
    41161 ttttcaccca acaaataacg ggccagcgtc gtgatgacga cgccttcttt tttgataaac
    41221 gttcgacgca taatatattt tcaataaaat gaatatttat atcttcatta aaatgaagat
    41281 aagtggcaac gacgagatgt ggaaacggcg aggcgcttca cattttttcg gattattgca
    41341 aatgaggata aaaaaaccgg gtttccccgg tttttggcat taatgttaaa ttacggcttc
    41401 gccacaaaac caatcgcttc gtacaccgct ttcagcgtac gggaagcgtg tacactggct
    41461 ttttctgcgc cgtctttcat cacctgttgc aggaaggctt catcgttgcg gaaacggtga
    41521 tagcgttcct gcaattcagt cagcataccg gaaacggcat cagccacttc acctttcaga
    41581 tgaccataca tcttgccttc gaactgtttt tccagttctg ggatgctctg gcccgttacc
    41641 gcagaaagga tatccagcag gttggaaacg cccgctttgt tctgcacatc gtagcgaact
    41701 accggcggct cgtcggagtc agtgaccgcg cgtttgattt tcttcactac cgatttcgga
    41761 tcttccagca ggccgataac gttattgcgg ttatcgtcag acttggacat cttcttggtc
    41821 ggctccagca gcgacattac gcgcgcgcca gatttcggaa taaacggctc cggcacctta
    41881 aagatctcgc catacagcgc gttgaaacgc tgggcgatat cgcgactcag ttccaggtgc
    41941 tgtttctggt cttcacccac cggtaccaga ttagtttgat acagcaggat gtccgcagcc
    42001 atcagcaccg gatagtcaaa cagaccagcg ttgatgttct cggcataacg cgcagattta
    42061 tctttaaact gcgtcatgcg actcagttcg ccgaagtagg tataacagtt cagtgcccag
    42121 cctaactgcg catgttctgg tacgtgggac tgaacaaaaa tggtgctttt ctcaggatcg
    42181 ataccacaag ccagatacaa ggccagcgta tccagcgtcg ctttacgcag cttctgtgca
    42241 tcctggcgca cggtgatcgc gtgttggtca acgatacagt aaatgcaatg gtagtcatcc
    42301 tgcatgttta cccactgacg cagcgcaccc atgtagttac caatggtcaa ttcacctgag
    42361 ggctgtgcgc cactaaaaac gatgggctta gtcatttttc gattcctgat tttcgctatg
    42421 cggaagccct aatgcgggca gaaggtcatt tatagactga taaattacat caggctggct
    42481 gagatcgata gcctcgccgt agttatatcc gtaggttaag ccaactgatg ggcaacctgc
    42541 cgcttttgcc gcctgaatat cattgcgtga gtcgccgaca aacagcatct gttgtggggc
    42601 aattcccatc cgctcagcca ccagtaacag cgggtccgga tgcggttttt tgttttgcac
    42661 atcatcgccg ccgataacca cgctaaagta tttggcaata tctaaggctt cgagcagcgg
    42721 tgcgacgaac ggcgtcggtt tgttggtgac caggcctagc ggcaggcctt tagcctgcaa
    42781 cgcgcccaac gtatcggcaa cgtgcgggaa caaaaacgtc ccctcttcgg caacctcgcc
    42841 atagtagcga tcgaacagtt tacgcagaat acgtacctgt tcttctgccg gaatgtcgtc
    42901 atcaacgggc ggtttaccca ttgttttacg ctgagtcgca cgttcctgac gcgcccaggt
    42961 caatgcgcgc tccatcagaa catctgcgcc gttaccaatc caggtaataa cgcgttcttc
    43021 acctgcgatg ggcaactcca gcgcatacag cgccatatct accgcagcag caagaccggg
    43081 agcactgtcg accagcgtac catcaagatc aaaagcgacg ccgcgaatat cttcaaactt
    43141 attcatgact tacctttgcc agttcactgc gcatttcatc aatgactttt ttgtagtctg
    43201 gctggtcgaa gattgccgaa ccggcgacga acatatccgc gcccgccgca gcgatttcgc
    43261 caatgttgtt caccttcacg ccaccgtcca cttccagacg aatgtcaaag ccagactcgt
    43321 cgatacggcg acgtacttcg cgcagtttat ccagtgtttg aggaatgaaa gactgaccgc
    43381 cgaaaccagg attgacggac atcagcagga tcacatccag cttatccatc acgtaatcca
    43441 gatagctcag aggtgtcgcc gggttaaata ccagacccgc tttacagcca ttttctttaa
    43501 tcagttgcag cgtgcggtca acatgctcgg aggcttctgg atgaaaggta atgatgctgg
    43561 caccagcggc agcgaaatca ggcacaatgc gatcgacggg tttcaccatc agatgtacgt
    43621 cgataggggc ggtaatgcca tagttacgca aggatttcag caccattggc ccaatcgtca
    43681 gattgggaac atagtggtta tccatgacgt caaaatgcac gacatcagcg ccagctgcca
    43741 gggcttttgc ggtatcttca cccaggcggg caaaatcagc cgacagaatt gagggggcaa
    43801 tcaaatactg tttcatccgc ttctccttga gaattatttt ttcgcgggtg aaacgactcc
    43861 tggtttgtac aaagccagca gttcgtccac ctttttacgt gtgccgccgt tgctgcttat
    43921 actgcgtcga actttgacga catgcaattt tgcgcgctga taccactcac gcgttaacat
    43981 cgtatcgtga ttggagatca gcactggaat atggcgctca accagacctt cggcgatctc
    44041 cgccagatgc gcttgttgtt caagcgtaaa actgtttgtg tgatacgccg taaagttggc
    44101 ggtcgcagac agcggtgcat aaggcggatc gcaatagacg acggatgcat catctgcgcg
    44161 cgccatgcta tcggcgtaag actcacaata gaaaaaggca ttctgcgctt tttcagcgaa
    44221 gtgatacaac tctgcttccg ggaaataggg ttttttgtag cggccaaacg gcacgttaaa
    44281 ctcaccgcgc agattgtaac gacacaggcc gttgtaaccg tagcggttca aatataaaaa
    44341 cagtaccgcc cgacggaacg gatcctggct tttgttgaac tcttcgcgga actgatagta
    44401 aacctcggcg caatttgttt cgggaacaaa cagctcgcgt gcggcctgta cgtactcatc
    44461 agtacgcatc ttcacaatgt tatagagact gatcaggtcg ctattgatat cggcaaggat
    44521 ataacgagaa aagtcggtgt tgagaaacac cgacccggca cctacaaaag gctcaaccag
    44581 acattcgccc ttgggcaaat gccgtttaat atcatcaagc aggggatact tgccccctgc
    44641 ccacttcaaa aaagcgcgat ttttcttcat gctgactaac taattacacc ttctccggct
    44701 gtggagaaag ctccgacagc atccagcgct tcaggcagct cccgcaacga taaaccattg
    44761 cgggagataa ccttgattac ttcagatcgg cctgtacctg acgcagcggt ttcgcccacg
    44821 ggtttttggc ctgaacatct gctggcaatg tagataccgc ttttttcgcc tcttctttcg
    44881 aagcgtacac gccagaaacc aggacatacc acggctgacc attacgcgcc gtttcataga
    44941 caacgtagtt tttcagattc tctttcttcg cccaaccgtt caggttgtcg tagttagagg
    45001 aactgctcag ctgcagagtg taatggctgg acggtgccga tttcaacgaa ccaacattac
    45061 ctgcggtctt ccctccagcg gctggtgttg ccgtggtttg cgccggggat gccgtctgta
    45121 ctggagccgt agtcgccgtc tcttttggtg ctggcgtaga agtcgcagtc ggtgccttcg
    45181 tgctcgccac aggggcagct ggttcagtac gcttcggcgt ctgtgctacc ggcttcggct
    45241 ccgttttcac ggttgcttgc ggttttttcg gttcaatcac cgcctgctga cgagctgggc
    45301 gcgtagtgga cggacgttca gcggtttgcg ttttcgccgt gtcacgcgat gcattgccat
    45361 tgcgaacagg cgcgacagtc gcgggttcgg tcggcaatgt ggaattgacc gccacattgt
    45421 tcaactgttg ctgattttgt ggctgggtca gcgcattgtt caggtcaccc tgcacttcaa
    45481 cacgttgttg accatccgtt gccgccgggg tttgcccttg agtcggcgta gaagagatcg
    45541 gtggcagaga aacatcctgc tgagtatttt ccgcagacgt ggttcccggc gctggctgca
    45601 ccccattcgc ctgatcggtc gcattgcctg caagatcaat actcttctcg ccagacgcgg
    45661 tttgatcgct ggaagaggtc gagggggctt ttagcgcaga accgataccg atgatcaaca
    45721 gcagtagaac cagaatgccg acgcccatca tcatatactg acgagaagcg ggtttactgg
    45781 ctgctttttt gcgcttacgc ggacgacgct ctacgcgctc ttcatccacg gtttcatctt
    45841 cggattcgtc aatttcttct tcgatttccg gttcctcatt gcgctctttt tgcgcacgag
    45901 tcggacggcg atcgtcagtg tcatcaagtt caatatcatc aaaattgatc tgcggttcgc
    45961 cacgttcagt acgctcagaa cgttcagaag attgacgaga acgaccagta cgacgatcgc
    46021 tgggatcggg tttcagctcg tcttctggtt tgaattcatc catttaacac cccactaaaa
    46081 ggttaatgct taccacgttg caattaacct gaagctaata gaccgcttga taagcggcct
    46141 gacctttctt gttgttacgc tgattgacaa tcggcaatgg cgttaagaac aagctcgtgc
    46201 gaaacgccgc tgcgaacttc actcttacca attgccaacg gaagaattaa gcgtatctct
    46261 cccgcaagga ctttcttgtc acgcagcata tgcggtaaat acgcctgcgc ggacatttcg
    46321 cgcggcccat tgaccggtaa cccagcccgc ttgagcaggg ttataatacg ctgcgtttcg
    46381 gcagaactaa actgcccgag acgttccgac gtccgcgccg ccatcaccat acccgcagcg
    46441 accgcttcac catgtaacca attgccatac cccatttcag cttcaatggc atgaccaaag
    46501 gtgtgtccca gattcagtaa agcacgtaac ccggtttcgc gctcgtcggc ggcgacaact
    46561 tctgccttca gttcacaaca acggcgaata cagtacgcca ttgccggacc gtccagacgc
    46621 aacaacgcat ccagattctc ttccagccag ttaaaaaacg caccgtcaag aataatgccg
    46681 tatttgatga cttctgccag ccccgacgct aactcacgcg ggggaagcgt tttcagacag
    46741 tcgagatcca ccaccactga agcaggctgg tagaacgcgc caatcatgtt tttaccgagg
    46801 ggatggttga ccgcagtttt gccgccaacg gaggaatcga cctgcgacag taacgtcgtc
    46861 gggacttgaa tgaaacgaac accgcgctga taactcgccg ccgcgaagcc ggtcagatcg
    46921 cccactacgc cgccgccaag cgccaccagc gtagtatcgc gaccatgcgg cttttgtaac
    46981 aacgccgtaa agacggtatc gagtacagcc aggcttttat actgctcgcc gtcagggagg
    47041 ataacgctat cgacattaac acccgcctgt tcaagtacgc cgcggacctt atcgagatac
    47101 agaggagcca gggtttcgtt ggtgaccaac atgacctgct cgcccgattt cagcggtaag
    47161 aatgaagctg gttcattaaa caaaccagat gcgatggtaa ttgggtaact acgttccccg
    47221 agagtaacaa caatcctctc cataacgcga catccacctt aattactgta cccgcagacg
    47281 agtgtatata aagccagaat tagttgcttt ccagcatgtg aataatctgg tttgcaacca
    47341 ctttagcgct ttgatcatca gtacgaatgg tcacgtcggc aatctcttca tacagcggat
    47401 tgcgttcatt ggccaacgct tccagaactt cacgtggcgg tgtttcaacg tgcagcaacg
    47461 ggcgtttttt atcacgctgc gtgcgtgcaa gttgcttttc gatggtcgtt tcaagataaa
    47521 cgacaacgcc acgagcggaa agacggttac gcgtttcacg ggatttcaca gagccgccgc
    47581 cagtagccag cacaataccc tgtttctcgg tcaactcatt gatgaccttt tcttcgcgat
    47641 cgcggaagcc ttcttcgcct tctaaatcga aaacccagcc cacatcagct ccggttcgtt
    47701 tctcaatctc ttgatcggaa tcgtaaaatt ccatattgag ttgttgagct aactggcgcc
    47761 caatagtgct ttttccggca cccataggcc caaccagaaa gatattgcgt ttctctgcca
    47821 ttttttcggt actactaaga ctattcgtta atgataaacc cgcttcgctc agagagcgcc
    47881 gcaggacatg aactgaaacc tcataagata ttgcgagagt cagactgaaa attatctcaa
    47941 tactccagcg ggtttggcaa ctgaataaat cacccatccc tctgcatatc tggtcgctgc
    48001 aagcgcgctg ccttgctacc accgctctgg cgataaatca ccgggtaaga ttagcgtaaa
    48061 aaagacagca aaatgccgcc tgaatgataa atcatcatca tggaacgcag gcagcgaaga
    48121 gcaccaactc tcaaatcggt actccttgta tgctaaatac ctgcgcgcgt caaatagatg
    48181 aaacacgttc agcgtaaaaa cattaccgct tttacggctg tttactcact ggaaaccagt
    48241 cgtggcgtga taaacaccac taactcgcgt cgttcatctt cttttccgtc atgacgaaat
    48301 aattgcccga accagggaat gtcgccaagc aacggtacgc tatcctgacc cgatttattt
    48361 ttacgggtaa aaatgccgcc cagcgccaac gtttctccgc ttttgacctc gacctgcgtt
    48421 tcgatctcct gcttatcaat cgccagcact tcgccatcgg cctgctgtag cacctgcccc
    48481 ggaacgttct ggctgatgtg taatttcagc cggatgcgac ctttttgtaa cactgtgggc
    48541 gtaacttcca tccccaggac ggcctcttta aattccaccg acgtcgcgcc actttccccg
    48601 ctggaaacct gatatggaat ttcgctcccc tgtttaatgc tggcaggctg aagatgtgag
    48661 gccagcagac gcggactggc gataatatcc agctgctgtt tttgttcgag cgcggaaagc
    48721 tcaagatcca gcaagcgtcc gttgatgcgc ccaatgttaa aaccgacatg cgttgtcgcc
    48781 gtcgctacgg agaggtcgct accaagcgtg gtgacttgcc caacgccacc agcgtgttgc
    48841 gcatcggcca gcgtccattt cacgcctaac tcacgcaaac ttttttcatt aatggtgaca
    48901 atatgcgccg acagctcaac ctgcccgacc ggcagatcca tttgcgctac ccactgttca
    48961 agcgcgctta acgccgtttt gttatctcgt agcaaaaggc gattggtgcg tttatcgacg
    49021 gtcatactcc ctttggcact cagtagcttc tcccccgctt tcgccagttc tcccgcgtcg
    49081 gcgtattgca gggttatact gcgattttcc agcggcagat ttgcctgcgc ccgcgcctgc
    49141 tccgcctcct ggcgggcgat attgttattc tgccaggcaa tggaatgcac tgagagaatg
    49201 ttgccttcct gccgcgttat cagtccggcg cttttcacta cagtttgtag tgcctgcttc
    49261 cagggaacat ctgttagatg taacgacacc gtaccgctga cgtctggcga cacgaccagg
    49321 ttcaacttct cctgttcagc cagcgcctgc aacacctgag ctaccggaac gtcatccacc
    49381 atcagcgtca ctttttgcgg ctttgccgcc tgtacgccgg gtatcagcat caacagtagt
    49441 gcggctatcc attgcttcat ttgtatctcc ttgccgttgc cacaaccatt gtggcggttc
    49501 gcagtttgtc ccggtaccca gcgttagtac gtctggcgtc agctgtaaaa ttgtccagcc
    49561 gttttccagc acatcgtttt gctgcacccg tcgccatttc ttttgcccgt cttttattac
    49621 accgatgatg cgctcgcctc gccctaccat cccctgatag cgccactggc taagttcgct
    49681 aatccggcat agatcttccg gcggtttaaa agggtcacgc ataccggtta aaaggcacaa
    49741 tacaatacct gccaacaacc agcgtttaac cctcatgcgg cgtctccagt tgtagcgtga
    49801 acaaaagatc atcaccttcc acgcttaatg aaaaacggct cacgctgacg ttgcgctctg
    49861 ccagccgtgt aaatgccgac ggcactgctt cccacagcgt tttcaacgcc aactcgcctc
    49921 cctgcgcgga tggatgccag gaaaccagtt gcgcgccggg caactgaaaa tccagtggcg
    49981 aaaagggcag cgttttttct tcgctgaagg gagtggtgtc aaccaggcga tacagtgtgg
    50041 cccactgctg gtgatgactt gcccgtagtc gaattaatgc gtcgcgctct tcatggtgtg
    50101 tcgatgacag aaaaatgagc gtaactaaca tcagcaacca gaatgcccag caaaactggc
    50161 ggaggcgggg tgatgtggcg aaccaccagt caaagaacat gttcatcgct aaccttcctt
    50221 gttaactgat actcaaattg ccagcgtccc tgcgcatcct gctgcgtggc tccccgctga
    50281 ttgagatgaa aagacgcatc ctggcggagt gacgtttcta gtgcgtttaa cgcggtaatg
    50341 cttgttgtca gccccttgat ctccagcgtt ccctgctgcc agcttatcgt tgtcagccag
    50401 gcgtgctcgg gtaaaagcgc cgccagtgct tccagcgcag attgccagtc gcgggtaaat
    50461 tgtcgctggc gctgccgttg caaacgctgc tcacgaattt gttggcgctc cagcaaacgt
    50521 ggtttcgtta tctgtaagct gcgggcgagt tgttgttccg cctgaagcaa aacggtgttt
    50581 acgcgtgctt cggcgttgtc tgtcagacgc agtattagcg ttatcccgac ggccagcagc
    50641 agaggcgcaa cgaacatcag caaccagaaa cgaagaaaag cggtccggcg ttgctgtcgc
    50701 cagggcaaaa aattaattgg cgggttcatc agtacgcctt cccaagcgcc agccccagcg
    50761 cgatggcaaa gtctccacct ggcggcggta gcggcggctg acgaacagaa acggcctccc
    50821 agggatcaaa tccgccttcg ccacatatcg cgacgctttc gggatcaacg gataacgctg
    50881 ccgccagctc cttcgcgcta gtcatcccca ccgccagttt gcgcccccag cgacagcgtg
    50941 tcgcccacag ccactgttcg ttatcacgcc aggccagaca ttgctgatga gaaggtaaaa
    51001 aaggcaggaa tcgctgtaat gcactggcat ccggggtgat cgcgctcaca tgaacacgca
    51061 acctttctgc cagagtgagt agcgttgcca gctctttgct ttgcgcggca gtcacgttat
    51121 aggcgggact gagtgagtct tcgctgtaat cgaaacgcag ggagtccgga tccatatcca
    51181 gctcgcgggc catcgtccct gacagccagg ccgtttgctc ccgctcaccg agggacatcg
    51241 acgggcgcgg aaatgaccgc tgtaatgtgc gactggcggg aaacgccaac ataatgtgat
    51301 gacgctgcgg cagttcgcga ctccacggta acaacgtttt agccagccgc tgcgcatcaa
    51361 caatccgccc atctttgata atgtcgttct ccagcggcaa ccgccaccag cgttgcaaaa
    51421 agcattcttt tgcgccccgc acgatcgcaa ccgctactgc ttcttgctgt tgtaaatgca
    51481 aaccaatttg ccagatctta aatgccattg tgatgatctc cttatcaccc gtcactctga
    51541 cgggtatatc aatgcgtctg gcttgccttt atactaccgc acgtttgttt ataaactgcc
    51601 caaatgaaac taaatgggaa atttccagtg aagttcgtaa agtatttttt gatccttgca
    51661 gtctgttgca ttctgctggg agcaggctcg atttatggcc tataccgcta catcgagcca
    51721 caactgccgg atgtggcgac attaaaagat gttcgcctgc aaattccgat gcagatttac
    51781 agcgccgatg gcgagctgat tgctcaatac ggtgagaaac gtcgtattcc ggttacgttg
    51841 gatcaaatcc cgccggagat ggtgaaagcc tttatcgcga cggaagacag ccgcttctac
    51901 gagcatcacg gcgttgaccc ggtggggatc ttccgtgcgg caagtgtggc gctgttctcc
    51961 ggccacgcgt cacaaggggc gagtaccatt acccagcagc tggcgagaaa cttcttcctc
    52021 agtccagaac gcacgctgat gcgtaagatt aaggaagtct tcctcgcgat tcgcattgaa
    52081 cagttgctga cgaaagacga gatcctcgag ctttatctga acaagattta cctcggttac
    52141 cgcgcctatg gtgtcggtgc tgcggcacaa gtctatttcg gtaaaacggt cgaccaactg
    52201 acgctgaacg aaatggctgt gatagccggg ctgccgaaag cgccttccac ctttaatccg
    52261 ctctactcaa tggatcgtgc cgtcgcgcgg cgtaacgtcg tactgtcgcg gatgctggat
    52321 gaagggtata tcacccaaca acagttcgat cagacacgca ctgaggcgat taacgctaac
    52381 tatcacgcgc cggagattgc tttctctgcg ccgtacctga gcgaaatggt gcgccaggag
    52441 atgtataacc gttatggcga aagtgcctat gaagacggtt atcgcattta caccaccatc
    52501 acccgcaaag tgcagcaggc cgcacagcag gcggtacgta ataacgtgct ggactacgac
    52561 atgcgccacg gctatcgcgg cccggcaaat gtgctgtgga aagtgggcga gtcggcgtgg
    52621 gataacaaca agattaccga tacgctgaag gcgctgccga cctatggtcc gctgctgcct
    52681 gccgcagtca ccagcgccaa tcctcaggaa gcgacggcaa tgctggcgga cgggtcgact
    52741 gtcgcattga gtatggacgg cgttcgctgg gcgcgtcctt accgttcgga tactcagcag
    52801 ggaccgacgc cgcgtaaagt gaccgatgtt ctgcaaacgg gtcagcaaat ctgggttcgt
    52861 caggttggcg atgcatggtg gctggcacaa gtgccggaag tgaactcggc gctggtgtcg
    52921 atcaatccgc aaaatggtgc cgttatggcg ctggtcggtg gctttgattt caatcagagc
    52981 aagtttaacc gcgccaccca ggcactgcgt caggtgggtt ccaacatcaa accgttcctc
    53041 tacaccgcgg cgatggataa aggtctgacg ctggcaagta tgttgaacga tgtgccaatt
    53101 tctcgctggg atgcaggtgt cggttctgac tggcagccga agaactcacc accgcagtat
    53161 gctggtccaa ttcgcttacg tcaggggctg ggtcagtcga aaaacgtggt gatggtacgt
    53221 gcaatgcggg cgatgggcgt cgactacgct gcagaatatc tgcaacgctt cggcttcccg
    53281 gcgcaaaaca ttgtccacac cgaatcgctg gcgctgggtt cagcgtcctt caccccaatg
    53341 caggtggcgc gcggctacgc ggtcatggcg aacggcggct tcctggtgga cccgtggttt
    53401 atcagcaaaa ttgaaaacga tcagggcggc gtgattttcg aagcgaaacc gaaagtagcc
    53461 tgcccggaat gcgatattcc ggtgatttac ggtgatacgc agaaatcgaa cgtgctggaa
    53521 aataacgatg ttgaagatgt cgctatctcc cgcgagcagc agaatgtttc tgtaccaatg
    53581 ccgcagctgg agcaggcaaa tcaggcgtta gtggcgaaga ctggcgcgca ggagtacgca
    53641 ccgcacgtca tcaacactcc gctggcattc ctgattaaga gtgctttgaa caccaatatc
    53701 tttggtgaac caggctggca gggtaccggc tggcgtgcag gtcgtgattt gcagcgtcgc
    53761 gatatcggcg ggaaaaccgg taccactaac agttcgaaag atgcgtggtt ctcgggttac
    53821 ggtccgggcg ttgtgacctc ggtctggatt ggctttgatg atcaccgtcg taatctcggt
    53881 catacaacgg cttccggagc gattaaagat cagatctcag gttacgaagg cggtgccaag
    53941 agtgcccagc ctgcatggga cgcttatatg aaagccgttc ttgaaggtgt gccggagcag
    54001 ccgctgacgc cgcctccggg tattgtgacg gtgaatatcg atcgcagcac cgggcagtta
    54061 gctaatggtg gcaacagccg cgaagagtat ttcatcgaag gtacgcagcc gacacaacag
    54121 gcggtgcacg aggtgggaac aaccattatc gataatggtg aggcacagga attgttctga
    54181 ttaaaaaggc gcttcggcgc cttttcagtt ggctgacaaa gtgcatttgt ttatgccgga
    54241 tgcgggtgaa cgcgttatcc ggctaacaaa atcgtgaaaa atcaatatat tgcaggaacc
    54301 ccataagcct gataatcgtt gtgcatcagg caaacttcac gcatttacac tcgcccctgc
    54361 cctttcaacc attcgcgcac gaggaacagc gcgctgacat tacgcgcttc gttgaagtca
    54421 gggtcttcca gcaaatccat catatgcgcc agcggccagc gcacctgtgg tagcggctct
    54481 ggctcatcgc cttccagcga ttccgggtag agatcttgcg cgaccacgat attcattttg
    54541 ctggaaaagt aagacggtgc catgctgagc ttcttcaaaa aagtcagatc gttcgcacca
    54601 aatccaacct cttcttttag ctcgcggtta gcggcttcgt agacgctttc acccggatca
    54661 attaatcctt tcgaaaaacc taattcgtag gattcagttc ccactgcgta ttcgcggatc
    54721 aggatcaggt gatcgtccac aatcggcaca atcatcactg cttcccggtt ggttggacgc
    54781 attcgttcat aaacacgccg cacgccattg ctgaactcca gatccacgct ctcgacggta
    54841 aacagtcggg aacgggctac agtttcaaca ttcagaatgg tgggtttttg taatgatttg
    54901 ctcatcgtgg gatctatgct gtgaaatcag cggttattgt gcgatatcgg acacgctttc
    54961 ggcaatgtga attgcatgtt atttacattt atgtaactta ataaataatc gtcctcaaat
    55021 caaattaaaa gtcaataggt tgaaataact ccaggaattt gctgatatcc cgccttcaga
    55081 gggtttgcta tgatcagcgg ttactgtgat gtgcttaatg atgctcaagt taaactccac
    55141 gcttgccgat agccaaccgc agaatcatgt attgtgtctg gtgcgactga ccacgcctga
    55201 cagactaagt aagatgggga aagcatgagc accattgtga tttttttagc tgctttgctg
    55261 gcctgctcac tacttgcggg atggctgata aaagtgcgat ccagacggcg tcagctgccc
    55321 tggaccaacg ccttcgcgga tgcgcaaacg cgtaaactca cacctgaaga acgtagcgcc
    55381 gttgaaaatt atcttgagag cctgacgcag gtattacagg tgcctggccc aacgggagct
    55441 agcgcggcac cgatctctct ggcgctgaat gccgaaagca acaacgtcat gatgctgaca
    55501 cacgctatca cgcgttacgg catctctacc gacgatccga ataaatggcg ttactacctc
    55561 gattcggtag aagtccacct gccccctttc tgggaacaat acatcaacga tgagaatacc
    55621 gttgaactga tttataccga ttcgctgccg ctggttattt cactcaacgg tcatacgctg
    55681 caggagtaca tgcaggaaac gcgcggctat gccttgcaac ctgttccgtc cacgcaggcg
    55741 tcgattcgcg gggaagagag tgagcaaatc gagctactca atattcgcaa agaaacgcat
    55801 gaagaatatg cgctgagtcg tccgcgcggg ctgcgtgaag cgttgctgat cgtcgcctcc
    55861 ttcctgatgt tctttttctg cctgattacc ccggatgtat ttgttccgtg gctggcaggc
    55921 ggcgcattac tgctgctggg cgcaggtctg tgggggctat tcgcgccccc ggcaaaatcc
    55981 tccctgcggg aaattcattg tctgcgcggt acaccccgtc gttggggatt gtttggcgaa
    56041 aacgatcagg aacagatcaa caatatttca ctcggtatta tcgacctggt ctatcccgca
    56101 cactggcagc catacattgc tcaggatctc ggtcaacaaa ccgatattga tatctatctc
    56161 gaccgccacg tagtgcgtca ggggcgatat ctttcgctgc atgatgaagt aaaaaacttt
    56221 ccgttacagc actggctgcg cagtacgatt atcgctgcgg gctcgctgct ggtgctgttt
    56281 atgctgttat tctggatccc gctggatatg ccgctgaaat tcactctctc atggatgaaa
    56341 ggcgcacaga ccattgaagc caccagcgta aaacaactgg ctgacgctgg cgtgcgggta
    56401 ggcgatacat tgcgtatcag cggtacggga atgtgtaata ttcgcacttc cggaacctgg
    56461 agcgcgaaaa ccaattcacc ttttttaccg tttgactgct cgcagatcat ctggaacgac
    56521 gcccgctcat tgccattacc agaatctgaa ctggtcaaca aagcgacggc attgactgaa
    56581 gcagttaatc gccagctgca ccctaaaccg gaagatggat ctcgcgtcag tgcctcatta
    56641 cgttcagcaa ttcaaaaatc cggcatggta ttgcttgatg attttggcga cattgtactg
    56701 aagacagcgg atttatgttc tgccaaagat gactgtgtgc gactgaaaaa tgcgctggtc
    56761 aatctcggca acagtaaaga ctgggacgcg ctggtaaaac gcgccaacgc cgggaagctc
    56821 gatggcgtga atgtgttatt acgcccggtg agtgcggaat cgctggataa cctggtggca
    56881 acctccaccg cgccgtttat cacgcatgaa acggcgcgag cggcacaatc actaaacagt
    56941 ccggcccccg gcggattcct gattgtcagc gacgaaggca gcgattttgt tgatcagccc
    57001 tggccttcgg catcacttta cgactacccg ccgcaagaac agtggaacgc tttccagaaa
    57061 ctggcacaaa tgctgatgca tacgccgttt aacgccgagg gtatcgtcac aaaaatcttc
    57121 actgacgcca atggtacgca gcatattggc cttcatccaa tcccggatcg ttccggcctg
    57181 tggcgctatc tcagcaccac attgctgcta ctgacgatgc tgggtagcgc catttacaat
    57241 ggcgtacagg cctggcgtcg ttaccagcgt catcgcactc gcatgatgaa gattcaggcc
    57301 tattatgaaa gctgcctgaa cccgcaactg atcacccctt cagaaagcct tatcgaataa
    57361 cacgtttgcg cggcaggtta tgctaccctg tcgcgcaaat tgcttcactc tggagatttc
    57421 cctcatgcat atcaacattg cctggcagga cgtagatacc gttctgctgg atatggacgg
    57481 cacgttgctc gacctcgcct tcgataacta tttctggcaa aagctggtgc ctgaaacatg
    57541 gggcgcgaaa aacggggtta cgccacagga agcgatggaa tatatgcgcc agcaatatca
    57601 cgacgtacag catacgctaa actggtactg tcttgattac tggagtgagc aactgggtct
    57661 ggatatctgt gcgatgacca ccgagatggg accgcgtgcc gtactgcgtg aagataccat
    57721 tccgtttctt gaggcactga aagccagcgg taagcagcga attttgctca ccaatgcgca
    57781 tccgcacaac ctggcggtaa aacttgagca taccggtctg gacgcacacc ttgatttatt
    57841 actttccacc cacacatttg gttatccgaa agaggatcag cggttatggc atgcggtggc
    57901 cgaagctacg ggtctgaaag ctgaaagaac gctgtttatt gatgacagcg aagcgattct
    57961 cgatgctgcc gcgcaatttg gtattcgtta ctgcctcggc gtgactaatc ctgattccgg
    58021 gattgccgag aaacagtatc aacgccatcc gtcactgaat gactaccgcc gcctgatccc
    58081 ctcgctaatg tgaaggagac gccatgaaag aaaaacctgc tgttgaggtt cgactggata
    58141 aatggctatg ggctgcccgt ttttataaaa cccgcgcgct ggcccgtgaa atgattgaag
    58201 gcggtaaggt gcattacaac gggcagcgca gcaagccgag caaaatcgtc gagctgaatg
    58261 ccacgctcac tctgcgccag ggaaatgacg aacgcacggt gattgtaaag gcgattactg
    58321 aacagcgtcg ccccgccagc gaggcagcct tgctgtatga agagactgcg gaaagtgtag
    58381 agaaacgcga aaaaatggcg ctggcacgta aacttaatgc cttaaccatg ccgcacccgg
    58441 accgacgccc ggacaaaaaa gagcgccgcg acctgttacg atttaaacac ggcgacagtg
    58501 aataactgtc acctgcaaga gagatgatta tgccgcaaca tgaccaatta catcgctatc
    58561 tgtttgaaaa ctttgccgtg cgcggcgaac tggtaaccgt ttcggaaacc ctgcaacaga
    58621 tccttgagaa tcacgattat ccgcagcccg ttaaaaacgt gctggcagaa ctgctggttg
    58681 cgaccagcct gttaaccgct acgctgaagt ttgatggtga tatcaccgta cagctgcaag
    58741 gcgacggtcc gatgaatctg gcggttatta acggtaacaa taaccagcaa atgcgcggtg
    58801 tggcgcgcgt gcagggcgaa attccagaaa atgccgacct gaaaacgctg gtcggcaatg
    58861 gttacgtggt gatcaccatt accccgagcg aaggcgaacg ctatcagggc gtggttggtc
    58921 tggaaggtga taccctggcg gcctgcctgg aagattactt tatgcgttct gaacagctgc
    58981 cgacgcgcct gtttattcgc accggcgacg tagacggcaa accggctgca ggcggtatgt
    59041 tgttgcaggt aatgcctgcg caaaatgccc agcaggacga ctttgaccac ctggcgacgc
    59101 taaccgaaac catcaaaacc gaagaactgc tgaccttacc ggcaaacgaa gtgttgtggc
    59161 gtttgtatca cgaagaagag gtgacggttt acgatccgca ggatgtggag ttcaaatgca
    59221 cctgctcgcg tgaacgttgc gccgacgcgc tgaaaacgct gcctgatgaa gaagtcgata
    59281 gcatcctggc ggaagatggc gaaattgaca tgcattgtga ttactgcggt aaccactatc
    59341 tgttcaatgc gatggatatt gctgaaatcc gcaacaacgc gtctccggca gatccgcaag
    59401 ttcattaatg agtatgtccg gcagagaggg ctctgccgga ttttacgacg taatggcctg
    59461 atgtgctgcg cttaccaggc ctgtgaaata aatcgcaaat tattgttggt cagatacagc
    59521 gtcgtatccg ttaaaagagt tgtcacttac ttaagtttat ttccagcgtt tcaccatcta
    59581 ccgtcagatc cagttttcca tccttcgcag ctgtgagtaa tttttctttc gttatttcgt
    59641 tcggaagtaa actcatatca agcgcgtccc attcttttct gtctgagtca aagccataga
    59701 caatgactct gtcatcatta aaagcaaaca ggatccgctc cgcctgacca tcactattca
    59761 gatcttgctc gaccaggacg caggcgtctt tttcaataca ggtcatcacg ttatagcgat
    59821 cctggattaa ggccgaccag aatgtcgcat ccggtttaac agaaccagga gcaattaaca
    59881 cattttcggc taatactttt tccgatacct gttcctgaag atgttgctct ccatctaacg
    59941 ccatcagcag atcccgcgcg cgtttcgggt ctttcataaa cccggcatcg cttttcagcg
    60001 actcaagcgc cgcacgtcca tagcgaccgc tctgttcgag catgtagata gttacctggt
    60061 ctggcgtgtt tttgccgctc tgataacgcg ccatatgact gttcacgcta atgcgcatac
    60121 tgtccagcac cggcgaatta agaagcacca gtatcaccaa caccaataac gaaaccgcaa
    60181 ggttcacttt gccctgaaga acaaggggat tttgcccatt acgccagacg atactgacaa
    60241 aataccccag cgaccacacc agtaacacca gcaccgccag cgcgccctgc aagcggtcga
    60301 cagtccagcc gtactgagcg acccgcagcc ataacgccca ggcggcgacg aacacataca
    60361 gcggagcaac cagcaaagcg gttttaatca ggcaacgcaa cggccccgtc cagggaagtg
    60421 acgctttttg cggatcgcgg acaatagcca ttaagatcaa ttgcaaaaag gccagcgtta
    60481 acagcaaccc ggcggcggag atgtggcgag aaatcgcgct caggtccgta aacggcaggg
    60541 tgatgataaa catcagggtt agcaatgata ctaacggcag caaccccgtg gcgattagcg
    60601 tgaacaactt ttgaatagag tcgattaaac gtgactgtgt acgcgcgagg atcaccacca
    60661 gtgcggtaac cagaccaaat gtaagataca taaaccagtc ggttgcaaaa aagagcgtat
    60721 tgaaaaacgt gatgccaaca agtttaaaca actcacccca cagtaaaagc accagccacg
    60781 tcaggccatt ggagagaaaa atcaccagta atataagtac gttatgccat actgactggt
    60841 aaaagtagct atagcggcta ctgccattgc ggatacgaag gctttgttgt atccacggta
    60901 gcaacaacat tgccatcaac agcagatatc agccaaaatc ccaaatcgcc ttttcagctc
    60961 tccaggggtt catgccatca gtttgccact tcagccaacc gctcatccct gtcgtggcaa
    61021 taaacaccag cgccagccat ccccaaaggc gcttctgttt aaaagagatc acggagaaga
    61081 gtaaaacgga tgaaaacgcg acggtcgcag gcacgccata aacaatccaa ctgtgatttt
    61141 ttccggcaag ccaggcgata agcaggtagc aaaccagccc ctgaagtaat ccggtagcaa
    61201 tcatccccca gcgtgttgcc ggtgaaagtt caacgttatc catgttggtt atccagaatc
    61261 aaaaggtggg ttaattatcg catccgggca gtagtatttt gcttttttca gaaaataatc
    61321 aaaaaaagtt agcgtggtga atcgatactt taccggttga atttgcatca atttcattca
    61381 ggaatgcgat tccactcaca atattcccgc catataaacc aagatttaac cttttgagaa
    61441 cattttccac acctaaaatg ctatttctgc gataatagca accgtttcgt gacaggaatc
    61501 acggagtttt ttgtcaaata tgaatttctc cagatacgta aatctatgag ccttgtcgcg
    61561 gttaacaccc ccaaaaagac tttactattc aggcaataca tattggctaa ggagcagtga
    61621 aatgcgcgtt aacaatggtt tgaccccgca agaactcgag gcttatggta tcagtgacgt
    61681 acatgatatc gtttacaacc caagctacga cctgctgtat caggaagagc tcgatccgag
    61741 cctgacaggt tatgagcgcg gggtgttaac taatctgggt gccgttgccg tcgataccgg
    61801 gatcttcacc ggtcgttcac caaaagataa gtatatcgtc cgtgacgata ccactcgcga
    61861 tactttctgg tgggcagaca aaggcaaagg taagaacgac aacaaacctc tctctccgga
    61921 aacctggcag catctgaaag gcctggtgac caggcagctt tccggcaaac gtctgttcgt
    61981 tgtcgacgct ttctgtggtg cgaacccgga tactcgtctt tccgtccgtt tcatcaccga
    62041 agtggcctgg caggcgcatt ttgtcaaaaa catgtttatt cgcccgagcg atgaagagct
    62101 ggcaggtttc aaaccagact ttatcgttat gaacggcgcg aagtgcacta acccgcagtg
    62161 gaaagaacag ggtctgaact ccgaaaactt cgtggcgttt aacctgaccg agcgcatgca
    62221 gctgattggc ggcacctggt acggcggcga aatgaagaaa gggatgttct cgatgatgaa
    62281 ctacctgctg ccgctgaaag gtatcgcttc tatgcactgc tccgccaacg ttggcgagaa
    62341 aggcgatgtt gcggtgttct tcggcctttc cggcaccggt aaaaccaccc tttccaccga
    62401 cccgaaacgc cgcctgattg gcgatgacga acacggctgg gacgatgacg gcgtgtttaa
    62461 tttcgaaggc ggctgctacg caaaaactat caagctgtcg aaagaagcgg aacctgaaat
    62521 ctacaacgct atccgtcgtg atgcgctgct ggaaaacgtc accgtgcgtg aagatggcac
    62581 tatcgacttt gatgatggtt caaaaaccga gaacacccgc gtttcttatc caatctatca
    62641 catcgataac atcgttaagc cggtttccaa agcgggccac gcgactaagg ttatcttcct
    62701 gactgctgat gctttcggcg tgttgccgcc ggtttctcgc ctgactgccg atcaaaccca
    62761 gtatcacttc ctctctggct tcaccgccaa actggccggt actgagcgtg gcatcaccga
    62821 accgacgcca accttctccg cttgcttcgg cgcggcgttc ctgtcgctgc acccgactca
    62881 gtacgcagaa gtgctggtga aacgtatgca ggcggcgggc gcgcaggctt atctggttaa
    62941 cactggctgg aacggcactg gcaaacgtat ctcgattaaa gatacccgcg ccattatcga
    63001 cgccatcctc aacggttcgc tggataatgc agaaaccttc actctgccga tgtttaacct
    63061 ggcgatccca accgaactgc cgggcgtaga cacgaagatt ctcgatccgc gtaacaccta
    63121 cgcttctccg gaacagtggc aggaaaaagc cgaaaccctg gcgaaactgt ttatcgacaa
    63181 cttcgataaa tacaccgaca cccctgcggg tgccgcgctg gtagcggctg gtccgaaact
    63241 gtaatgattt gaagctggag aatatctatc cagtatctta tagaaagcaa aacgggaggc
    63301 accttcgcct cccgtttatt tacccttctt ttgtcgtgcc ctgcgcccgc gttaccggca
    63361 ctggcagcca ggcgcgaatg gaaagcccgc cccgctcgct ggtgccaagc tccagcatcc
    63421 cgttatggtt atccacgata cgctgcacaa ttgccagccc taatcccgtg ccgctaatgg
    63481 tgcgcgcact gtcgccgcgg acaaacggct ggaacaggtg cttacgttgt tccggcgcaa
    63541 ttcccggacc gtcatcttcc acctggaacc aggcgcgatt cggctccgtt ccgctgctga
    63601 ctttgatcca gccattgcca taacgagcgg cgttgaccac catattcgcc accgcgcgtt
    63661 tgatcgacag cgggtgcatt ttcacttcaa tgctgccggg gtaaagcgcg gtttcaattt
    63721 cccgctcata gccactttcg gcagcaatca cctcaccgag cactgcatta agatccgcca
    63781 tttccatcgg catctcctgc ccggtgcgca ggtagtcgat aaactgctca atgatggcgt
    63841 tgcactcttc gatatcttta ttgatcgatt ctgccagata gccatcctgc tcgctcatca
    63901 tctcagtcgc caggcgaata cgcgtcagcg gcgtgcgcaa gtcgtgactt acccccgcca
    63961 tcagcagcgt gcggtcatcc gccagttgct taacaccagc cgccatatgg ttaaaggcgc
    64021 gggtaacgga acgcacctcg gacgcgccat actcacgcag cggcggcgga ataatccctt
    64081 taccgacctg caaagcagca tgttcgagat cgaccaacgg tcggttctgg atacgaataa
    64141 acagccacgc cccgcctatc gccaatagca taatcgccag cgtatagcgg aacagcggag
    64201 agaaatcgcc ctgatgaatt tcggtcagcg gcacgcgtac ccagatattg ggcgacagcc
    64261 aggttttcag ccagacgaca ggcgaacttt tgttgacctc aacgcgcact tccgtcgggc
    64321 cgcccagttg ctgtgccatc tgatggctta agaattcata gtgttgcgcc caacgcagac
    64381 ctgcctcttc ggcagcctcg ttggagtaga gagagatccc cagctcacgg tagatctccc
    64441 gacggaaagc gggaggcaca accaactgcg tgccgtcctc cagttgcagt ttgtcggtca
    64501 tcaacatacg cacttcgtac gcgaggactt tattaaactg ctggaggctc ggcaaaatcg
    64561 cgaagttcag caccaccaga taagtcgtca ccaggctggc gaacagcaag gtgacgatga
    64621 gcaataacgt acgggcaaat gaacttcgtg gcgagaagcg caatcgcctc atgctttaga
    64681 gccgtccggt acaaagacgt agcccaggcc ccaaacggtc tgaatgtaac gcggatgcgc
    64741 tggatcttct tctaccatgc ggcgcagacg cgaaatctgc acgtcgatgg agcgttccat
    64801 tgcggaatat tcacgaccac gggcaaggtt catcagctta tcgcgggaga gcggctcacg
    64861 cggatggctg accagtgcct tcagtaccgc aaactcaccg ctggtgagcg gcatcggctc
    64921 atcttcgcgg aacatttcgc gcgtaccgag gttaagtttg aacttaccga aagcaattac
    64981 cgcctcttcc tgtgacggtg cgcctggcag ttcgttcgcc tgacgacgca gcaccgcacg
    65041 aatacgggcc agcagttcac gcgggttaaa cggttttgga atgtagtcgt cagcgccaat
    65101 ctccaggcct acgatacggt ccacttcttc ccctttcgcc gtcaccataa tgatcggcat
    65161 cgggttgctc tgactacgaa gacgtcggca aatcgacaag ccatcttcac caggtaacat
    65221 taaatccagt accataagat ggaaagattc acgagtcagc aggcgatcca tctgttctgc
    65281 attagcgacg cttcgaacct ggaagccttg ttcggtgaga taacgttcca gcagcgcacg
    65341 caggcgcatg tcgtcatcga ccaccagaat cttgtagttc tcttgcattg tttgtactcc
    65401 caaaggttcg caacaatttg taagcgtgta ttcttaaaaa agctcacgtt cgtcaccagc
    65461 taaatctggt atgaatttca gcctaaattg ttacaaagca tattaaacag cagcttaagt
    65521 atacaattta ttcggcgaaa cattattgat tctgttgata tgatcacgtt atacccaatg
    65581 tgcgcattat caaacagaca aagggaatca acgagatgaa aacgcccctg gttacccggg
    65641 aagggtatga aaaactcaaa caagagctta attatctctg gcgtgaagaa cgcccggagg
    65701 tcacaaaaaa ggtgacctgg gccgcaagtc tgggcgaccg cagcgaaaat gctgactatc
    65761 agtataataa aaagcgtctg cgtgaaatcg accgtcgcgt gcgctatctc actaaatgca
    65821 tggaaaatct caaaatcgtc aattactccc ctcagcagga aggcaaagtc ttttttggcg
    65881 cgtgggtgga gattgaaaac gacgatggcg tgactcaccg tttccgtatt gtcggctacg
    65941 atgaaatttt tggccgtaaa gattacatct ctatcgattc cccgatggcc cgcgcattgc
    66001 tgaaaaaaga agtcggcgat ctggcggtgg tgaatacccc tgccggggaa gcgagctggt
    66061 atgttaatgc tatcgaatac gtgaaaccgt aatgaagagt cttaacctcc tgccgatggc
    66121 tggcattttt gccagccagt ccgtataact atcccctgat ttttgatccg aaaagatgaa
    66181 ctcaaaccat gatgaatgat tcgttctgcc gcattattgc gggtgaaatt caggcgcgcc
    66241 cggaacaggt tgacgctgcc gttcgcctgc ttgacgaagg gaataccgtg ccgtttatcg
    66301 cacgttatcg taaggaaatc accggcggtc tggatgacac gcagctgcgt aatctggaaa
    66361 cgcgtctgag ctatctgcgc gagctggaag agagacgtca ggcgatcctc aagtccattt
    66421 ccgagcaagg caaactcacc gatgatctgg cgaaggccat caacgccacc ctaagcaaaa
    66481 ccgaactcga agacctctac ctgccctaca aacctaaacg ccgcacccgc gggcaaatcg
    66541 ccattgaagc agggcttgag ccgttggctg acctgctgtg gagcgatccg tcacacacac
    66601 cagaagtcgc cgctgcgcaa tatattgatg ccgataaagg cgtggcagat accaaagccg
    66661 cgctggacgg cgcgcgctat atcctgatgg aacggtttgc cgaagatgcc gcgctgctgg
    66721 cgaaagtgcg tgattatctg tggaagaacg cgcatttggt ttctacggtg gtgagcggta
    66781 aagaagagga aggggcgaaa ttccgcgact atttcgatca tcacgaaccg ttgtccacgg
    66841 tgccttctca ccgcgcgctg gcgatgttcc gtgggcgtaa cgaaggcgta ctccagcttt
    66901 cgctgaatgc cgatccgcag ttcgatgagc cgcccaaaga gagctattgc gagcaaatca
    66961 tcatggatca ccttggcctg cgcctgaaca atgccccggc ggatagctgg cgcaaaggcg
    67021 tagtgagctg gacctggcgc atcaaggtgc tgatgcatct ggaaaccgaa ctgatgggta
    67081 ccgtgcgcga acgcgcggaa gatgaagcaa tcaacgtctt tgcccgtaac ctgcacgatc
    67141 tgctgatggc ggcccctgcc ggactgcgtg caacgatggg cctcgatccg ggtctgcgta
    67201 ccggggtaaa agtggcagtg gtcgatgcca ctggcaaact ggtggcgacc gataccattt
    67261 acccgcacac cggacaagcc gcaaaagtag cgatgaccgt tgctgccttg tgtgaaaaac
    67321 ataacgttga actggtggcg atcggcaacg gtacagcttc ccgcgaaact gaacgtttct
    67381 atctcgacgt gcagaagcag ttcccgaaag tgaccgcaca gaaagtgatc gtcagcgaag
    67441 ctggcgcgtc ggtttactca gcttccgagc tggcagcgca ggagttcccg gatctcgacg
    67501 tttcgctgcg tggtgcggta tctatcgccc gccgtttgca ggatccgctg gcggagctgg
    67561 tgaaaatcga tccgaaatct atcggcgtag gtcagtatca gcatgacgtc agccagacgc
    67621 aactggcccg caaactggat gcagtagtag aagactgcgt aaacgccgtt ggcgtcgatc
    67681 tcaacaccgc ttctgttccg ctattaactc gcgtggcggg cctgacgcgc atgatggcgc
    67741 aaaacatcgt ggcctggcgc gatgagaacg gccagttcaa gaaccgtcag caactgctga
    67801 aagtcagccg tctggggccg aaagccttcg agcagtgcgc gggcttcctg cgcattaacc
    67861 acggtgataa cccgctggac gcctctaccg ttcacccgga agcctatccg gtggtggaac
    67921 gcattctggc agcaacacag caggcactga aagatctgat gggtaacagc agcgaactgc
    67981 gtaacctgaa agcgtctgac tttactgatg aaaaattcgg tgtgccgaca gtaactgaca
    68041 tcatcaaaga gctggaaaaa ccgggtcgcg atccgcgtcc ggaatttaaa accgctcagt
    68101 ttgccgatgg cgtcgagaca atgaacgacc tgcaaccggg tatgatcctc gaaggcgctg
    68161 tcaccaacgt caccaacttt ggcgcgtttg tcgatattgg cgtgcatcag gacggcctgg
    68221 ttcacatctc ttcattgtcg aacaagtttg tggaagatcc gcataccgtg gtgaaagcgg
    68281 gcgacattgt gaaggtgaaa gtgctggaag tggatcttca gcgtaaacgt atcgccctga
    68341 ccatgcgtct ggacgagcag cctggcgaaa ccaacgcccg tcgcggcggc ggtaatgaac
    68401 gcccgcaaaa caaccgcccg gcagccaaac cacgcggtcg tgaagcgcag cctgccggta
    68461 atagcgcgat gatggatgcg ctggcggcgg caatgggcaa aaaacgttaa acgcccggac
    68521 tggcccctaa acctgcgcga taaggcgttc acgccgcatc tggcaacgaa cgccttgcct
    68581 gacataaaag tgccggagaa tatctccggc ctttttattc cacaaccaaa ctcataatat
    68641 attccggcaa tatttatcac ttcattaaca actgaaacct taattaaaca ttagccagtc
    68701 cgggtaattc actattcgaa ttatattttc gctgcgatat aaccttgagc cacatcaaca
    68761 ttgagtcaga ttattattca aaccaacatt cgcacacatt ttaagtattg ctgatagaaa
    68821 ccattctcat tatcattgtg ttgttgatta tttaatctct cctttgttgg caaatcatct
    68881 ggtctcatgt cgctgtcaaa cgccccatga ggtagttatc cagttaatga gaaacaagta
    68941 ggcacctatg caatacactc cagatactgc gtggaaaatc actggctttt cccgtgaaat
    69001 cagcccggca tatcgccaaa aactgctttc tcttggcatg ttacctggct cctcttttaa
    69061 tgtggtgcgc gtcgctccac tcggcgaccc cattcatatc gaaacccgtc gtgtgagcct
    69121 ggtattacgc aaaaaagatc tggccttatt agaagtggaa gcggtttcct gttaatacgg
    69181 tgataacaac aatgaaaaaa ttaaccattg gcttaattgg taatccaaat tctggcaaga
    69241 caaccttatt taaccagctc actagcgcac gtcagcgtgt aggtaactgg gctggcgtta
    69301 ccgtcgaacg taaagaaggg caattctcca ccaccgatca tcaggtcacg ctggtggacc
    69361 tgcccggcac ctattctctg accaccatct catcgcagac ctcgctcgat gagcaaatcg
    69421 cctgtcacta cattttgagt ggcgacgccg acctgctgat taacgtggtg gatgcgtcta
    69481 accttgagcg taacctgtac ctgacgctac aactgctgga actcggcatt ccctgcatcg
    69541 tggcactgaa catgctcgac attgccgaga agcaaaatat tcgtattgaa attgatgctc
    69601 tgtcggcgcg tcttggctgc ccagtgatcc cgctggtttc aacccgtggt cgcggtattg
    69661 aagcgctcaa gctggcgatt gatcgctata aagctaacga gaatgtggaa ctggtgcatt
    69721 acgcacagcc gctactcaac gaagcagatt cactggcaaa agtgatgcct tccgacatcc
    69781 cgctgaaaca acgtcgctgg ctgggcctgc aaatgctgga aggcgatatc tacagccgcg
    69841 cctacgccgg tgaagcgtcg cagcatctgg atgccgccct tgcccgtctg cgtaatgaaa
    69901 tggatgatcc ggcgctgcac attgccgatg cgcgttacca gtgcatagct gccatctgtg
    69961 atgtggtaag caacaccctg acggcagaac ccagccgttt caccactgcg gtagataaaa
    70021 tcgtgctcaa ccgtttcctc ggtctgccga ttttcctctt tgtgatgtac ctgatgttcc
    70081 tgctggctat caacatcggc ggggcgttac agccgctgtt tgacgtcggc tccgtggcgc
    70141 tatttgtgca tggtattcaa tggattggct acacgctcca cttcccggac tggctgacta
    70201 tcttcctcgc ccagggcctg ggtggcggta ttaacaccgt gctgccactg gtgccgcaga
    70261 tcggcatgat gtacctgttc ctctccttcc ttgaggactc cggctatatg gcgcgtgcag
    70321 cgtttgtgat ggaccgcctg atgcaggcgc tgggcttgcc ggggaaatcc tttgtgccgc
    70381 tgatcgtcgg tttcggttgt aacgtaccgt cggtaatggg cgcacgtacg cttgatgcac
    70441 cgcgtgaacg tctgatgacc atcatgatgg caccgtttat gtcctgcggc gcgcgtctgg
    70501 ctatcttcgc agtattcgcg gctgccttct tcgggcagaa cggtgccctg gcggttttct
    70561 cgctgtatat gctcggtatc gtgatggcgg tgctgactgg cctgatgctc aagtacacca
    70621 tcatgcgcgg tgaagcgacg ccgtttgtca tggagctgcc ggtctaccat gtaccacacg
    70681 ttaaaagcct gattatccag acctggcagc gtctgaaggg cttcgttctg cgtgctggta
    70741 aagtgattat catcgtcagc attttcctga gcgctttcaa cagcttctcg ttgagcggga
    70801 aaatcgtcga taacatcaac gactcggcgc tggcgtccgt cagccgggtg ataacccctg
    70861 tcttcaagcc gattggcgta catgaagata actggcaggc aacggttggc ctgtttacgg
    70921 gcgcaatggc aaaagaagtg gtggtgggta cgctcaacac cctctacacc gcagaaaata
    70981 ttcaggacga agagttcaat ccggcggaat tcaacctcgg tgaagagctg ttcagtgcgg
    71041 tagatgaaac ctggcagagc ctgaaagaca ccttcagcct tagcgtactg atgaacccca
    71101 ttgaagctag caaaggcgac ggtgaaatgg gtaccggggc gatgggcgtg atggatcaga
    71161 aattcggtag cgcagctgcc gcatacagct acctgatttt cgtcctgctg tatgtaccat
    71221 gtatctcggt gatgggagcc atcgcccgtg aatcaagccg tggctggatg ggcttctcca
    71281 tcctgtgggg gctgaatatc gcttactcac tggcaacatt gttctatcag gtcgccagct
    71341 acagtcagca cccaacttac agcctggtgt gcattctggc ggtaatcctg tttaacatcg
    71401 tagttatcgg tctgctgcgc cgcgcgcgta gccgggtgga tatcgaactg ctggcaaccc
    71461 gcaagtcggt aagcagttgc tgcgcagcca gcaccaccgg tgattgccat taatggcttc
    71521 acttattcag gtacgtgatt tgctggcgtt acggggccgt atggaagcga cccagataag
    71581 ccagacattg aacactccgc agccaatgat taacgccatg ctgcaacaac tggaaagtat
    71641 gggcaaagcc gtgcggattc aggaagaacc tgacggctgc ctctctggca gttgtaaaag
    71701 ctgcccggaa ggaaaagcct gtctgcgcga gtggtgggcg ctgcgttaac cttactccat
    71761 cgcctgtttt ggaaagcccg gtatgcgtct gcatccgggc ttttttgcgt gcggctttcc
    71821 ataaaaatgc aactcttgca gcacggcgta agttcctttg aaagcatctc gcagggatga
    71881 aaactcgcta atgcacaggt gtggagtggc gcgtagagtc gcggcattca aacaacaggt
    71941 gaaggaacgc catgagcaaa aagcagagtt ccaccccaca cgatgcgctg ttcaaactct
    72001 ttttacgcca accggacacg gctcgtgatt ttcttgcgtt tcatttaccg gcacccattc
    72061 acgcgctttg tgatatgaaa accctcaagc tggagtcgag cagctttatt gatgacgatc
    72121 tgcgtgaaag ctattccgat gtgctgtggt cggtgaaaac ggaacaagga ccaggataca
    72181 tctattgtct gattgaacat caaagcacct caaacaaact gatcgcattt cgcatgatgc
    72241 gttacgctat tgccgcaatg caaaatcacc ttgatgctgg atacaaaacg ttgccgatgg
    72301 tggtgctatt gttgttttac cacggtattg aaagccccta tccctattcg ctgtgttggc
    72361 tggattgttt cgccgatccc aaactggcaa ggcagcttta tgcctccgca tttccgctga
    72421 ttgatgtcac cgtcatgcct gatgatgaaa tcatgcagca ccgacgtatg gcgctgctgg
    72481 agttaattca aaaacatatt cgtcaacgcg
//
LOCUS       DAKISA010000007        71352 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.007, whole genome shotgun sequence.
ACCESSION   DAKISA010000007 DAKISA010000000
VERSION     DAKISA010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 71352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71352
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.007"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..637
                     /locus_tag="OVW45_000465"
     CDS             <1..637
                     /locus_tag="OVW45_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCV5524997.1"
                     /translation="SAWAAGKGGIGLAATRLVYSEGEEQISLGVRNTSPDVPYLIQSW
                     VMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGAPLAKDRETLFFTSVRAVPSTTK
                     REEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLRCTSSADMVTVSNPTPYFITLTD
                     LKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRTINDYGAETPQLNCKS"
     gene            662..3261
                     /locus_tag="OVW45_000466"
                     /pseudo
     CDS             662..3261
                     /locus_tag="OVW45_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3252..4322
                     /locus_tag="OVW45_000467"
     CDS             3252..4322
                     /locus_tag="OVW45_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCV5524998.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDAFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            4334..4876
                     /locus_tag="OVW45_000468"
     CDS             4334..4876
                     /locus_tag="OVW45_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCV5524999.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAASITGADVLFSGEADTLTP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            4884..5399
                     /locus_tag="OVW45_000469"
     CDS             4884..5399
                     /locus_tag="OVW45_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCV5525000.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            5365..6102
                     /locus_tag="OVW45_000470"
     CDS             5365..6102
                     /locus_tag="OVW45_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCV5525001.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            6213..7223
                     /gene="pyrD"
                     /locus_tag="OVW45_000471"
     CDS             6213..7223
                     /gene="pyrD"
                     /locus_tag="OVW45_000471"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCV5525002.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            7397..7939
                     /gene="zapC"
                     /locus_tag="OVW45_000472"
     CDS             7397..7939
                     /gene="zapC"
                     /locus_tag="OVW45_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCV5525003.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7936..9045)
                     /gene="ycbX"
                     /locus_tag="OVW45_000473"
     CDS             complement(7936..9045)
                     /gene="ycbX"
                     /locus_tag="OVW45_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCV5525004.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            9289..11397
                     /gene="rlmKL"
                     /locus_tag="OVW45_000474"
     CDS             9289..11397
                     /gene="rlmKL"
                     /locus_tag="OVW45_000474"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCV5525005.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRTGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHMALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            11409..13316
                     /locus_tag="OVW45_000475"
     CDS             11409..13316
                     /locus_tag="OVW45_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCV5525006.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            13446..14699
                     /gene="pqiA"
                     /locus_tag="OVW45_000476"
     CDS             13446..14699
                     /gene="pqiA"
                     /locus_tag="OVW45_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCV5525007.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14704..16344
                     /gene="pqiB"
                     /locus_tag="OVW45_000477"
     CDS             14704..16344
                     /gene="pqiB"
                     /locus_tag="OVW45_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCV5525008.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGLE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKVRLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            16341..16904
                     /gene="pqiC"
                     /locus_tag="OVW45_000478"
     CDS             16341..16904
                     /gene="pqiC"
                     /locus_tag="OVW45_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCV5525009.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            17160..17327
                     /gene="rmf"
                     /locus_tag="OVW45_000479"
     CDS             17160..17327
                     /gene="rmf"
                     /locus_tag="OVW45_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCV5525010.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(17397..17915)
                     /gene="fabA"
                     /locus_tag="OVW45_000480"
     CDS             complement(17397..17915)
                     /gene="fabA"
                     /locus_tag="OVW45_000480"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCV5525011.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17984..19744)
                     /locus_tag="OVW45_000481"
     CDS             complement(17984..19744)
                     /locus_tag="OVW45_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCV5525012.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19930..20382
                     /gene="matP"
                     /locus_tag="OVW45_000482"
     CDS             19930..20382
                     /gene="matP"
                     /locus_tag="OVW45_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCV5525013.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(20458..21498)
                     /gene="ompA"
                     /locus_tag="OVW45_000483"
     CDS             complement(20458..21498)
                     /gene="ompA"
                     /locus_tag="OVW45_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCV5525014.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(21853..22362)
                     /gene="sulA"
                     /locus_tag="OVW45_000484"
     CDS             complement(21853..22362)
                     /gene="sulA"
                     /locus_tag="OVW45_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCV5525015.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            22581..23210
                     /gene="sxy"
                     /locus_tag="OVW45_000485"
     CDS             22581..23210
                     /gene="sxy"
                     /locus_tag="OVW45_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCV5525016.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23173..25335)
                     /gene="yccS"
                     /locus_tag="OVW45_000486"
     CDS             complement(23173..25335)
                     /gene="yccS"
                     /locus_tag="OVW45_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCV5525017.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(25345..25791)
                     /locus_tag="OVW45_000487"
     CDS             complement(25345..25791)
                     /locus_tag="OVW45_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCV5525018.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            25914..27968
                     /gene="helD"
                     /locus_tag="OVW45_000488"
     CDS             25914..27968
                     /gene="helD"
                     /locus_tag="OVW45_000488"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCV5525019.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28000..28458)
                     /gene="mgsA"
                     /locus_tag="OVW45_000489"
     CDS             complement(28000..28458)
                     /gene="mgsA"
                     /locus_tag="OVW45_000489"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCV5525020.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(28554..29216)
                     /locus_tag="OVW45_000490"
     CDS             complement(28554..29216)
                     /locus_tag="OVW45_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCV5525021.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            29389..29802
                     /locus_tag="OVW45_000491"
     CDS             29389..29802
                     /locus_tag="OVW45_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCV5525022.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(29847..30164)
                     /gene="hspQ"
                     /locus_tag="OVW45_000492"
     CDS             complement(29847..30164)
                     /gene="hspQ"
                     /locus_tag="OVW45_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCV5525023.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(30222..31412)
                     /gene="rlmI"
                     /locus_tag="OVW45_000493"
                     /pseudo
     CDS             complement(30222..31412)
                     /gene="rlmI"
                     /locus_tag="OVW45_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
     gene            31507..31785
                     /gene="yccX"
                     /locus_tag="OVW45_000494"
     CDS             31507..31785
                     /gene="yccX"
                     /locus_tag="OVW45_000494"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCV5525024.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(31782..32111)
                     /gene="tusE"
                     /locus_tag="OVW45_000495"
     CDS             complement(31782..32111)
                     /gene="tusE"
                     /locus_tag="OVW45_000495"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCV5525025.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(32202..32861)
                     /gene="yccA"
                     /locus_tag="OVW45_000496"
     CDS             complement(32202..32861)
                     /gene="yccA"
                     /locus_tag="OVW45_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCV5525026.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(33068..33155)
                     /locus_tag="OVW45_000497"
     tRNA            complement(33068..33155)
                     /locus_tag="OVW45_000497"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33119..33121),aa:Ser,seq:tga)
     gene            33582..34700
                     /gene="hyaA"
                     /locus_tag="OVW45_000498"
     CDS             33582..34700
                     /gene="hyaA"
                     /locus_tag="OVW45_000498"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCV5525027.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAAWPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDCGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            34697..36490
                     /gene="hyaB"
                     /locus_tag="OVW45_000499"
     CDS             34697..36490
                     /gene="hyaB"
                     /locus_tag="OVW45_000499"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCV5525028.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            36509..37215
                     /gene="hyaC"
                     /locus_tag="OVW45_000500"
                     /pseudo
     CDS             36509..37215
                     /gene="hyaC"
                     /locus_tag="OVW45_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
     gene            37212..37799
                     /locus_tag="OVW45_000501"
     CDS             37212..37799
                     /locus_tag="OVW45_000501"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCV5525029.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            37796..38134
                     /gene="hyaE"
                     /locus_tag="OVW45_000502"
     CDS             37796..38134
                     /gene="hyaE"
                     /locus_tag="OVW45_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCV5525030.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPGYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHP"
     gene            38191..39048
                     /locus_tag="OVW45_000503"
     CDS             38191..39048
                     /locus_tag="OVW45_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCV5525031.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPISCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            39182..40726
                     /gene="appC"
                     /locus_tag="OVW45_000504"
     CDS             39182..40726
                     /gene="appC"
                     /locus_tag="OVW45_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCV5525032.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKTVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLPLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            40738..41874
                     /gene="appB"
                     /locus_tag="OVW45_000505"
     CDS             40738..41874
                     /gene="appB"
                     /locus_tag="OVW45_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCV5525033.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            41887..41979
                     /gene="cbdX"
                     /locus_tag="OVW45_000506"
     CDS             41887..41979
                     /gene="cbdX"
                     /locus_tag="OVW45_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCV5525034.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            42059..43357
                     /gene="appA"
                     /locus_tag="OVW45_000507"
     CDS             42059..43357
                     /gene="appA"
                     /locus_tag="OVW45_000507"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCV5525035.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHIQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(43472..45652)
                     /gene="etk"
                     /locus_tag="OVW45_000508"
     CDS             complement(43472..45652)
                     /gene="etk"
                     /locus_tag="OVW45_000508"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HCV5525036.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(45672..46118)
                     /gene="etp"
                     /locus_tag="OVW45_000509"
                     /gene_synonym="yccY"
     CDS             complement(45672..46118)
                     /gene="etp"
                     /locus_tag="OVW45_000509"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCV5525037.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     IKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(46106..47245)
                     /locus_tag="OVW45_000510"
     CDS             complement(46106..47245)
                     /locus_tag="OVW45_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCV5525038.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(47291..49387)
                     /locus_tag="OVW45_000511"
     CDS             complement(47291..49387)
                     /locus_tag="OVW45_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCV5525039.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDIYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(49387..50133)
                     /locus_tag="OVW45_000512"
     CDS             complement(49387..50133)
                     /locus_tag="OVW45_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCV5525040.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(50130..50774)
                     /gene="gfcB"
                     /locus_tag="OVW45_000513"
     CDS             complement(50130..50774)
                     /gene="gfcB"
                     /locus_tag="OVW45_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HCV5525041.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(50881..51186)
                     /locus_tag="OVW45_000514"
     CDS             complement(50881..51186)
                     /locus_tag="OVW45_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525042.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAASEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(51633..51845)
                     /gene="cspH"
                     /locus_tag="OVW45_000515"
     CDS             complement(51633..51845)
                     /gene="cspH"
                     /locus_tag="OVW45_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCV5525043.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            52131..52343
                     /gene="cspG"
                     /locus_tag="OVW45_000516"
     CDS             52131..52343
                     /gene="cspG"
                     /locus_tag="OVW45_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HCV5525044.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            52354..52542
                     /locus_tag="OVW45_000517"
     CDS             52354..52542
                     /locus_tag="OVW45_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCV5525045.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            52517..52747
                     /gene="ymcE"
                     /locus_tag="OVW45_000518"
     CDS             52517..52747
                     /gene="ymcE"
                     /locus_tag="OVW45_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HCV5525046.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            52737..52910
                     /locus_tag="OVW45_000519"
     CDS             52737..52910
                     /locus_tag="OVW45_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCV5525047.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(52959..54032)
                     /locus_tag="OVW45_000520"
                     /pseudo
     CDS             complement(52959..54032)
                     /locus_tag="OVW45_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(54104..56848)
                     /gene="torS"
                     /locus_tag="OVW45_000521"
     CDS             complement(54104..56848)
                     /gene="torS"
                     /locus_tag="OVW45_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HCV5525048.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHQEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     QLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            56931..57959
                     /gene="torT"
                     /locus_tag="OVW45_000522"
     CDS             56931..57959
                     /gene="torT"
                     /locus_tag="OVW45_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCV5525049.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(57932..58624)
                     /gene="torR"
                     /locus_tag="OVW45_000523"
     CDS             complement(57932..58624)
                     /gene="torR"
                     /locus_tag="OVW45_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCV5525050.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            58744..59916
                     /gene="torC"
                     /locus_tag="OVW45_000524"
     CDS             58744..59916
                     /gene="torC"
                     /locus_tag="OVW45_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCV5525051.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKDEK"
     gene            59916..62335
                     /gene="torA"
                     /locus_tag="OVW45_000525"
                     /pseudo
     CDS             59916..62335
                     /gene="torA"
                     /locus_tag="OVW45_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            complement(62974..63279)
                     /gene="cbpM"
                     /locus_tag="OVW45_000526"
     CDS             complement(62974..63279)
                     /gene="cbpM"
                     /locus_tag="OVW45_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCV5525052.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(63279..64199)
                     /gene="cbpA"
                     /locus_tag="OVW45_000527"
     CDS             complement(63279..64199)
                     /gene="cbpA"
                     /locus_tag="OVW45_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCV5525053.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNHQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            64460..65606
                     /locus_tag="OVW45_000528"
                     /pseudo
     CDS             64460..65606
                     /locus_tag="OVW45_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            65900..67141
                     /gene="agp"
                     /locus_tag="OVW45_000529"
     CDS             65900..67141
                     /gene="agp"
                     /locus_tag="OVW45_000529"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCV5525054.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(67179..67406)
                     /locus_tag="OVW45_000530"
     CDS             complement(67179..67406)
                     /locus_tag="OVW45_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCV5525055.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKADKDSLFWGEQTIERKNV"
     gene            complement(67427..68023)
                     /gene="wrbA"
                     /locus_tag="OVW45_000531"
     CDS             complement(67427..68023)
                     /gene="wrbA"
                     /locus_tag="OVW45_000531"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCV5525056.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            68396..68569
                     /locus_tag="OVW45_000532"
     CDS             68396..68569
                     /locus_tag="OVW45_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCV5525057.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(68827..70155)
                     /gene="rutG"
                     /locus_tag="OVW45_000533"
     CDS             complement(68827..70155)
                     /gene="rutG"
                     /locus_tag="OVW45_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCV5525058.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(70176..70670)
                     /gene="rutF"
                     /locus_tag="OVW45_000534"
     CDS             complement(70176..70670)
                     /gene="rutF"
                     /locus_tag="OVW45_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCV5525059.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPSGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(70681..71271)
                     /locus_tag="OVW45_000535"
     CDS             complement(70681..71271)
                     /locus_tag="OVW45_000535"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCV5525060.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYAPIKWGP
                     TSANCSPARIVFIRTVEGKERLHPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
BASE COUNT        17776 a        17684 c        18621 g        17271 t
ORIGIN      
        1 atccgcatgg gcggcaggta aaggcgggat tggacttgca gcaacacgtc ttgtttattc
       61 agaaggtgaa gagcaaattt cactgggtgt acgtaacacc agtccggatg ttccttatct
      121 tattcagtca tgggtgatga ccccagataa taaaaaatca gcagatttta ttattacacc
      181 accgctattt gtgctgaatc cggctaatga gaatctgtta cgcattatgt acattggtgc
      241 gccgttggcg aaagacagag aaaccctttt cttcactagc gtacgggcag tcccttcaac
      301 aacgaagcgg gaagagggaa ataccctgaa gattgccaca caaagcgtca tcaaactttt
      361 ctggcgacca aaaggtttag cgtatccctt aggcgaggct ccggcgaaac tgcgttgcac
      421 ttcgtcagct gacatggtta cggtcagtaa cccaacacct tatttcatta ccctgacaga
      481 cctgaaaata gggggaaagg tagttaaaaa tcaaatgatt tccccctttg ataaatacca
      541 attctctctg ccaaaggggg ccaaaaatag cagcgtaacg tatcgaacca tcaatgatta
      601 cggggcggaa acgccacaac tcaactgtaa atcgtaagcc gtcttcagtt aagagagcga
      661 gatgtataga actcatcgac aacacagcct gttaagctct ggtggagtgc catcgtttat
      721 tggtgggctg gtggtgtttg tgtcggcagc gttcaatgca caagctgaaa cctggttcga
      781 tcctgctttt ttcaaagatg atccctcaat ggtggccgat ttgtctcgtt tcgaaaaagg
      841 acaaaaaata acgccagggg tttatcgtgt cgatattgtt ctgaatcaga caattgtaga
      901 tacgcgcaac gtcaattttg ttgagataac gccagagaag gggattgccg cctgtttgac
      961 gactgaaagc ctggatgcaa tgggtgttaa tactgatgcg tttccggctt ttaaacaact
     1021 ggacaaacaa gcgtgtgtgc cattggcgga gattattccg gatgccagcg taacgtttaa
     1081 tgtgaataaa ctccgtctgg aaatttcagt accgcaaatc gccatcaaaa gtaatgcccg
     1141 tggttatgtc ccccctgaac gttgggatga agggatcaac gcgctattac tgggatattc
     1201 gtttagcggg gctaacagta ttcatagcag cgcagacagt gattctggcg acagctattt
     1261 tctgaattta aacagtggcg ttaatttagg cccatggaga ttgcgcaaca attcaacatg
     1321 gagtcgcagt agtggccaaa ccgcagaatg gaagaatctc agcagctatt tgcagcgggc
     1381 ggttattcca ctaaaaggcg aactgaccgt aggtgatgat tatactgcag gcgatttttt
     1441 cgatagtgtc agctttcgtg gtgtgcagct ggcgtcagat gacaacatgc tgccagacag
     1501 cctgaaaggg tttgcgcctg tggtgcgtgg tatcgccaaa agcaatgccc agataacgat
     1561 taagcaaaat ggttacacca tttaccaaac ttatgtatcg cctggtgctt ttgaaattag
     1621 tgatctctat tccacgtcgt cgagcggtga tttgttagtt gaaatcaaag aagcggacgg
     1681 cagcgtcaat agctacagcg taccgttttc cagcgtgcca ttactccagc gtcaggggcg
     1741 aatcaaatac gcggtgacac tggcgaaata cagaaccaat agtaatgaac agcaggagag
     1801 caaatttgcc caggccacgt tgcagtgggg cggaccgtgg ggaacgacat ggtatggtgg
     1861 tggacaatat gctgaatatt accgtgccgc catgtttggt ctgggattta accttggcga
     1921 tttcggagca atttcgttcg atgcgaccca ggcgaagagt acgctggcag accaaagcga
     1981 acataaaggt cagtcatatc gttttctgta tgccaaaacg ctcaaccaat tgggcactaa
     2041 ttttcaattg atgggctatc gctattcgac gtcgggtttc tacacccttt ccgacaccat
     2101 gtataaacat atggatggct acgaatttaa tgacggtgat gatgaagata cgccgatgtg
     2161 gtcgcgttat tacaatttgt tttacaccaa acgtggcaaa ctgcaggtca atatctccca
     2221 gcaattaggc gagtacggtt cgttttattt aagtggtagc cagcaaactt actggcatac
     2281 cgatcaacag gatcggctat tacagtttgg ctacaacacg caaattaaag atctctcgct
     2341 ggggggttcc tggaactaca gtaagtcccg tggtcaacct gatgctgatc aggtgtttgc
     2401 actaaatttt tccctgccgc tcaatctgtt gctccccaga agtaatgata gctataccag
     2461 gaaaaaaatt acgcctggat gacctctaac accagcatcg ataacgaagg gcacattaca
     2521 caaaacctgg gtttaacgga gacactactc gatgacggta acctgagcta cagcgtgcaa
     2581 cagggatata acagcgaggg gaaaacggct aatggtagcg ccagcatgga ctacaaaggg
     2641 gcgtttgcag atgcccgagt gggctacaac tacagcgata acggcagtca acaacaactg
     2701 aactacgctc tttcaggcag tttagttgcc cattcacagg gcattaccct ggggcaatcg
     2761 ctgggggaaa ctaacgtgct gattgcagca ccaggcgcag agaatactcg tgtggcgaac
     2821 agcaccgggc tgaaaactga ctggcgcgga tataccgttg tcccctatgc cacttcttat
     2881 cgggaaaatc gaatcgcact tgatgcggca tcgttaaaac gtaacgtgga tcttgaaaat
     2941 gcagtagtca acgtggttcc caccaaaggg gcgttggttc tggcggagtt caatgcccat
     3001 gcgggagcaa gggtattaat gaaaacatca aagcagggta taccgctgcg ttttggcgcg
     3061 atagcgacgc tggacggcgt acagactaat agcggcatta ttgatgatga tggctcgctc
     3121 tatatgtccg gtttgcccgc tcagggagcg ataactgtac gctggggcga agctcccgat
     3181 caaatttgtc atatcagtta ccagcttacc gaacaacaaa ttaactctgc gattacgcga
     3241 atggatgcca tatgcagata atctttggag aaaaatgcgt gttattacta cgactatttt
     3301 ttgccgccgt cttaatgcta tggtgcgctc aaactgctgc ttatagcggg cagtgtcata
     3361 ccactcaggg gaatccgtat attggcgtca attttggcgt taaaaccctg gaggaagaag
     3421 aaaatacggc tggagtagtt aaagacaaat tttatcagtg gaacgaatcg aatgattatt
     3481 atgtttcctg tgattgcgat aaagacaatg tcagaagtgg ccgatgggca ttcgccgcgg
     3541 attcaccgtt agtctattta ggcgacaact ggtacaaaat taatgactat cttgccgcca
     3601 aagttttatt gcaggttaaa ggcagttctc ctactgcggt tcctttcgaa aacgtgggca
     3661 caggggcaga tacacgatgg catatttgcg atcccggcgg tcaacgttta ggtggccagg
     3721 gggctagcgg taatagcggt agcttttccc tgaaaatatt gcagccgttc gttggttcgg
     3781 tcgtcattcc tcctatggcg ctggcgcgat tatttgaatg ctacaacata cccgcaggtg
     3841 attcctgcac gactacaggt acaccggttt tagtgtatta cctgtctggt acgatcaatt
     3901 cacttggctc atgttccgtc aatgccggag aaacaatcga ggtcgatctg ggcgacgcat
     3961 ttgcggctaa ctttcgtgtt gtagggcata agcctcttgg ggccagaacg gcagaacttg
     4021 caattccagt caggtgtaac acgggaaacg cggggttagt taacgtcaac ctgagtctga
     4081 cggcaaccac agaccccagc tatccccagg cgattaagac gtcacgtcct ggcgtgggcg
     4141 tggtggtgac cgatagccag aacaacatta tttcccctgc tggtggaaca ttaccgctct
     4201 ctattcctga tgatgcagac agtatcgcgc gaatgaatgt ctatccagtc agcacgacag
     4261 gtgtaccacc agaaaccggg cgatttgaag ccacggcaac ggtgagaata aattttgatt
     4321 aacgcgtgaa cgtatgaaga aaaaaacgat atttcagtgc gttatcttgt tctttagcat
     4381 tcttaacatc catgtcggga tggctgggcc tgaacaagtt agtatgcata tttatgggaa
     4441 tgtggtcgat cagggctgtg atgtcgccac caaaagtgca ttacaaaata ttcatattgg
     4501 tgattttaat atcagtgatt ttcaggccgc gaataccgta agcactgctg ctgatttgaa
     4561 tatcgatatc accggttgtg ccgctagtat tactggcgcg gacgtccttt ttagcggcga
     4621 ggctgacacc cttacgccga cactgctcaa actaactgac acaggcggaa gcggtggtat
     4681 ggcaacgggg attgccgtgc aaattcttga tgcgcaaagt cagcaagaaa tcccgctcaa
     4741 tcaggtccag cctcttacgc ccttaaaagc cggggataac acactcaaat atcaacttcg
     4801 ttataagtcc acaaaggcgg gagcaacggg cggtaatgcg acggcggttc tctattttga
     4861 tctggtttac cagtgaagga gtgatgttga aacggataat ctggattctg ttcttactgg
     4921 gattaacggg gggctgtgag ctatttgccc atgatggcac ggtcaacatt agcggatcgt
     4981 ttcgccgtaa tacatgcgtg ctggcacagg atagcaagca aatcaatgtg cagttaggcg
     5041 atgtctcgct gacacgtttt tttcatggga attatggccc ggagaaatct ttcatcatta
     5101 atttgcagga ttgcggaaca gatgtcagca cggttgacgt caccttttca ggaacccccg
     5161 acggagtgca gagcgaaatg ctgtccattg aaagtggtac ggatgcagcc agcgggctgg
     5221 caattgcgat tctggatgat gcgaagatat tgattccgct taaccaggcg agtaaagact
     5281 acagcctaca tagcggtaag gtgccattaa ctttttatgc gcaattgcga cctgtcaata
     5341 gcgatgtgca gtcgggtaaa gtgaatgcca gcgcgacatt tgtacttcat tatgactaat
     5401 acgtggaaca gattggcgct cttgattttc gccgttttat cgctgctggt ggcgggtgaa
     5461 ttgcgggcag gagtggtggt cggcggaacg cgatttatct ttccggcaga cagagaatcg
     5521 atatctattt tactgactaa taccagtcag gaatcctggc ttattaacag taaaatcaac
     5581 cgcccaacgc gttgggccgg gggggaagcg tcgacagtgc cagcaccatt actggccgct
     5641 ccgccactta ttctcctgaa gcccggtact acaggcacgt tgcgcttgct gagaacggaa
     5701 agcgacatct tgcctgtgga tcgcgaaacg ctatttgagt taagcattgc cagcgtgcca
     5761 tccggcaaag ttgaaaatca gagcgtaaaa gtggcgatgc gctcggtatt taaactgttc
     5821 tggcgacccg aaggtttgcc gggcgacccg ctggaagctt atcaacaatt acgctggaca
     5881 cggaattcgc agggtgtaca actcactaac ccaacgcctt actacattaa cctgattcag
     5941 gtgagcgtaa atggtaaagc gttaagtaat gcgggagttg tgccgcctaa aagccagcgt
     6001 cagacaagct ggtgtcaggc aatcgcaccg tgtcatgtcg cctggcgggc gataaatgat
     6061 tacggcgggt tgagtgcaaa gaaggagcaa aatctgccct gaaacaggtt cggaaaacgt
     6121 ttgcgttttt tttgccgcag gtcaattccc ttttggtccg aactcgcaca taatacgccc
     6181 ccggtttgca caccgggaat ccaggagagt tcatgtacta ccccttcgtt cgtaaagccc
     6241 ttttccagct cgatccagag cgcgctcatg agtttacttt tcagcaatta cgccgtatta
     6301 caggaacgcc gtttgaagca ctggtgcggc agaaagtgcc tgcgaaacct gttaactgca
     6361 tgggcctgac gtttaaaaat ccgcttggtc tggcagccgg tcttgataaa gacggggagt
     6421 gcattgacgc gttaggcgcg atgggatttg gatcgatcga gatcggtacc gtcacgccac
     6481 gtccacagcc aggtaatgac aagccgcgtc tctttcgtct ggtagatgcc gaaggtttga
     6541 tcaaccgtat gggctttaat aatcttggcg ttgataacct cgtagagaac gtaaaaaagg
     6601 cccattatga cggcgtcctg ggtattaaca tcggcaaaaa taaagatacg ccagtggagc
     6661 agggcaaaga tgactatctg atttgtatgg aaaaaatcta tgcctatgcg ggatatatcg
     6721 ccatcaatat ttcatcgccg aataccccag gattacgcac gctgcaatat ggtgaagcgc
     6781 tggatgatct cttaaccgcg attaaaaata agcaaaatga tttgcaagcg atgcaccata
     6841 aatatgtgcc gatcgcagtg aagatcgcgc cggatctttc tgaagaagaa ttgatccagg
     6901 ttgccgatag tttagttcgc cataatattg atggcgttat tgcaaccaat accacactcg
     6961 atcgttctct tgttcaggga atgaaaaatt gcgatcaaac cggtggctta agtggtcgtc
     7021 cgcttcagtt aaaaagcacc gaaattattc gccgcttgtc actggaatta aacggtcgct
     7081 taccgatcat cggtgttggc ggcatcgact cggttatcgc tgcgcgtgaa aagattgctg
     7141 cgggtgcctc actggtgcaa atttattctg gttttatttt taaaggtccg ccgctgatta
     7201 aagaaatcgt tacccatatc taattatttc ttcgaattcc tctttataag ataatcaggg
     7261 ctttattttc agccctggtt gttttatatt catcgctgtt gcttatttag acattttgta
     7321 cttttattgt tgaggttatt aagcgaagcg acaatggatt gtgttgctgc ggttttatag
     7381 ggtaggggag aggcagatgc gaattaaacc agacgataac tggcgttggt attacgatga
     7441 agagcacgat cgaatgatgc tcgatttagc caatggtatg ctatttcgct cacgttttgc
     7501 gcgcaagatg ttgactccag atgccttttc tcccgcaggc ttttgcgttg acgacgccgc
     7561 gctctatttc tcttttgaag aaaagtgccg cgattttaat ttatctaaag aacaaaaagc
     7621 cgaactggtg ctgaatgcac tggtagcaat tcgctatctc aaaccgcaaa tgccgaaaag
     7681 ctggcatttt gtttcccatg gtgaaatgtg ggtgcccatg ccgggcgatg cggcctgcgt
     7741 ttggttgagc gatactcacg agcaggtcaa tttgctggtc gttgaatctg gcgaaaatgc
     7801 cgcactatgc ctgctggcac aaccctgcgt tgttattgcg ggtcgcgcga tgcagttggg
     7861 cgacgccatt aaaatcatga acgacaggct gaaaccgcag gttaatgttg acagcttcag
     7921 cctcgaacag gcagtctaac gcgccaactt aagtgcagtc ttcggtacac agctacagca
     7981 aagaatggtg ccatcatcgc ccattgctga ttttttcagc ggcgtgactt cgccttctaa
     8041 aagctgaaca cggcaacttc cacaaatgcc cgcgcggcaa gaataaggga tacgaattcc
     8101 ctgattttct aattgctcca gcaacacctg ttggttattt ccacgaaatg cctgtccctg
     8161 ccagtcaata tctacatttg cgtccggttg ttgcgtgatg ttggcggtat catcagcggc
     8221 agctgcgccg taaatttttg ccggagccgt tgccagaatt tccacctcat cgccaacgcg
     8281 aatcacgccg ctattacggg caattaaatt ctgaccaaaa tcgacatcgc cgttatcctg
     8341 ggcagtgcgg aaagattgca atgtttttaa tggttcgcct gccggatgtt tttgcccttt
     8401 ttctgggctg acggtggtga aaatacagcg gctacaaggt ttaaccacat caaacaccac
     8461 atcaccaatg cgaatcactt tccagcgatc ttcttcccag gctgacgcgc cggaaaccac
     8521 cagattgggg cggaattgct ccatttttac actggccgga caacgttgtt ggagatcacg
     8581 taacgaggct tcgttagcaa gaaggtaagg atagccatca gcaaatgaca gaggtacagt
     8641 gttgtggcgt ttcacgcgcc gggtcatttg tggccccacc cagcgtaatt gcacttcgcg
     8701 ggagaaaaat ccacttagcc atttgttgat cgcgtctggc gcaattcgcg cggtaaaatg
     8761 tgtgccccaa acttcggttg gtgcgtcttg tgtggcgaaa tcagcaaaac gaacatatgc
     8821 gctactgcca tctggtgcgg tgagatgcaa gccatcatgc acaggtgaag gagtaaaccg
     8881 taccatctgg ggaaactggc gagcggtaat aaacgtaccg tcaggttccg tgatcataaa
     8941 gatgcgatcg aaggccagac cactgacatc agccagagca tgtgtaagac caatgccgcg
     9001 catcgattta acaggatgaa taaaaagccg ggttaatgtc gccacggtgc ggtcctcaaa
     9061 tgaaaataag ccctcaactt tatgacatga cgcgcttatt agctataatg cgcaacaatt
     9121 ttcttagcgc ctgtcccatt aggctatttt atttgtcagt ttggccctgg gttgtgctcg
     9181 aaatcctcac gtacttgtgt acgctccggt ttctccgcgc tgtccatgtc cagactgact
     9241 gcaacaatta cgcctactgc ggtaggctct tagagtaaaa gtgacgatat gaattctctg
     9301 tttgccagta cggcccgtgg gctggaagag ctgttaaaaa ctgaactgga aaacctgggg
     9361 gccgttgaat gccaggtggt tcagggtggg gtccatttca agggcgacac acggcttgtt
     9421 taccagagcc tgatgtggag ccgcctggcc tcgcgtatta tgttgccgct gggcgagtgt
     9481 aaggtttaca gcgatttaga cctctatctc ggtgttcagg cgatcaactg gacagagatg
     9541 tttaatcctg gcgcgacctt cgctgtccac ttcagtggtt tgaatgacac catccgcaac
     9601 agtcagtacg gcgcgatgaa agtgaaagac gcgatcgtcg atgctttcac gcggaaaaat
     9661 ctgccgcgtc caaatgttga tcgcgatgcg ccggatatcc gcgttaacgt ctggctgcat
     9721 aaagaaaccg ccagtatcgc tcttgatctg agtggtgatg gtttacatct gcgtggctat
     9781 cgcgatcgtg ctggtattgc gccgatcaaa gaaaccctgg cagccgctat tgtgatgcga
     9841 accggctggc agccaggaac accgctgctc gatccgatgt gtggttccgg tacgttgctg
     9901 attgaagcgg cgatgctagc gaccgatcgc gcaccaggct tgcaccgtgg gcgttggggc
     9961 tttagcggct gggtgcagca tgatgaagct atctggcagg aagtgaaagc ggaagcgcaa
    10021 actcgtgccc gtaaaggcct ggctgagtat agctctcatt tttacggttc ggacagcgac
    10081 gcacgggtga ttcaacgtgc gcgcactaac gcccgtcttg cggggattgg tgaactgatc
    10141 acctttgagg tgaaagatgt cgcgcaactg accaatccgc tgccgaaagg gccgtacggc
    10201 acagtgttga gcaacccgcc atacggggaa cgtctggaca gcgaaccggc gctgattgcg
    10261 ctgcatagcc tgctgggtcg gatcatgaaa aaccagtttg gtggctggaa tctctctttg
    10321 tttagtgcct cgccggatct gctaagctgc ttgcagctgc gtgcagacaa acagtacaag
    10381 gcgaaaaacg gcccgctgga ctgcgtacag aaaaattacc acgttgccga aagcactcca
    10441 gacagcaaac cggcgatggt agcggaagac tacgccaacc gcctgcgtaa gaacctcaaa
    10501 aaattcgaga agtgggctcg ccaggaagga attgaatgtt accgcctgta tgacgccgat
    10561 ctaccggaat ataacgttgc cgttgaccgt tatgccgact gggtggtggt gcaggagtat
    10621 gcgccgccaa aaactattga tgctcacaaa gcgcgtcagc gtctgttcga tattatcgct
    10681 gcaaccattt cggtactggg gattgcgcca aacaaactgg tgctgaaaac ccgtgaacgc
    10741 cagaagggca aaaaccagta ccagaaactg ggcgagaagg gcgagtttct tgaagttacc
    10801 gaatataacg ctcacttgtg ggtgaacctg acggattatc tcgatactgg tctgttcctc
    10861 gatcaccgta tcgcccgccg tatgctcggt cagatgagca aaggcaaaga tttcctcaac
    10921 ctgttctctt ataccggtag cgccaccgtg catgcgggat taggcggcgc gcgcagcacc
    10981 accaccgtgg atatgtcgcg tacttatctg gagtgggcag aacgcaacct gcgtctgaat
    11041 ggcctgaccg ggcgtgcgca tcgcctgatt caggccgatt gcctggcgtg gctgcgtgag
    11101 gcaaatgaac agtttgatct gatctttatc gatccgccaa ccttctctaa ctcaaaacga
    11161 atggaagatg cgtttgatgt tcagcgcgat catatggcgc tgatgaaaga tttgaaacgt
    11221 ctgctgcgtg caggtgggac gatcatgttc tcgaacaaca aacgtggctt ccgtatggat
    11281 ctcgacggcc tggcaaaact gggactgaaa gcacaagaaa ttacgcaaaa aacgctctcc
    11341 caggatttcg cccgtaaccg ccagatccac aactgctggc tgattaccgc agcctgaaag
    11401 gaatagtaat gtcattaatc agtatgcatg gcgcatggct gtcgttcagc gacgcgccgc
    11461 ttctcgataa cgcagaactg catatcgaag ataacgaacg tgtttgtctg gtgggccgca
    11521 acggcgcggg caaatcgacg ttaatgaaaa tcctcaaccg tgaacaaggg ctggatgacg
    11581 gtcgcattat ttacgagcaa gatttgattg tagctcgtct gcaacaggat ccgccgcgta
    11641 acgttgaggg tagcgtttat gatttcgttg ccgaaggcat tgaagaacaa gcggaatatc
    11701 tgaaacgcta tcacgatatt tcgcgcctgg tgatgaacga cccgagcgag aaaaatctca
    11761 acgaactggc gaaggttcag gaacagttgg atcaccacaa cctgtggcag ctggaaaacc
    11821 gcatcaacga agtgctggcg caactggggt tagatcctaa tgttgcgctg tcgtcgcttt
    11881 ccggcggctg gttgcgtaaa gcggcattag gacgcgcgct ggtgagtaat ccgcgcgtgc
    11941 tgttgcttga tgaaccgaca aaccacctgg atattgaaac catcgactgg ctggaagggt
    12001 tccttaaaac cttcaatggg acgattattt tcatctccca cgaccgttcg tttatccgca
    12061 atatggcgac gcgcattgtt gatctcgatc gcggcaagct ggtgacctat ccagggaatt
    12121 acgaccagta cctgcttgaa aaagaagaag ccctgcgcgt ggaagaacta caaaatgccg
    12181 agttcgatcg caaactggcg caggaagagg tgtggatccg ccaggggatc aaagcacgcc
    12241 gtacccgtaa tgaaggccgc gtacgcgccc tgaaagcaat gcgtcgcgag cgtggtgaac
    12301 gtcgcgaagt gatgggtacc gcaaagatgc aggtggaaga ggccagccgc tccggtaaga
    12361 tcgttttcga aatggaagac gtttgctacc aggttgacgg taagcaactg gtgaaagatt
    12421 tttcggccca ggttctacgt ggcgacaaaa ttgccctgat tggtccgaat gggtgcggca
    12481 aaaccacgct gctgaaactg atgctcggtc agcttcaagc ggacagcggg cgtattcacg
    12541 ttggcaccaa actggaagtg gcttatttcg atcagcaccg cgcggaactg gatcccgata
    12601 aaacggtgat ggataacctt gccgaaggta agcaagaggt gatggttaac ggcaagccac
    12661 gccacgtatt gggctatttg caggactttc tgttccatcc gaaacgggcg atgacgccgg
    12721 tacgtgcgct ttctggcggt gagcggaacc gcttgctgct ggcgcgtttg ttcctcaaac
    12781 caagcaactt attgattctt gacgaaccga ccaacgatct tgatgtcgaa acgctggaac
    12841 tgctggaaga actgatcgac agctatcagg gcacggtatt gctggttagc cacgatcgtc
    12901 agtttgtcga taacaccgtt acagaatgtt ggatcttcga aggcggcggt aaaattggtc
    12961 gttatgtcgg cggttatcat gatgcccgtg gtcagcaaga gcagtatgtg gcgctcaaac
    13021 agcctgcggt gaaaaaaacc gaagaagccg ccgcggcaaa agcagaaact gtaaaacgca
    13081 gcagtagcaa actaagctat aaattgcagc gcgaactgga gcagctaccg caattgctcg
    13141 aagatctgga ggcgaagctg gaagccctac agacgcaggt ggcggatgct tccttcttca
    13201 gtcagccaca tgagcagaca caaaaagtgc tcgccgatat ggctgctgca gagcaggagc
    13261 tggagcaagc ctttgaacgc tgggagtatc ttgaagcgtt aaaaaatggt ggctgatcgc
    13321 aaaatagtcg atttaaacag agacagctga caacggtaaa attgcctgat gaactgggtt
    13381 tatcaggcat gaaaaccaac actttgttag tcatctgtaa aatagcgcat cattaaggag
    13441 taccaatgtg cgaacatcat catgccgcga agcacatcct gtgctcgcag tgtgacatgc
    13501 tggtggcgtt accgcgcctt gagcatggtc agaaagcggc atgtccccgg tgtggcacaa
    13561 cgttaaccgt ggcgtgggat gcccctcggc agcgtccgac cgcctatgcg ttggctgcac
    13621 tgttcatgct gttgctgtcc aacttgtttc cttttgtgaa tatgaacgtt gcaggagtta
    13681 ccagtgaaat tacattactg gaaattcccg gcgtgctttt ttctgaggac tacgccagcc
    13741 tcggcacctt tttcctgttg tttgtgcaac tggttcccgc gttttgtctg ataaccattc
    13801 tgttactggt gaatcgcgcg gaattaccgg tccgtttaaa agagcaactg gcacgggtgc
    13861 tttttcaact caaaacctgg ggaatggcgg agattttcct cgctggtgtg ctggtcagtt
    13921 tcgttaaact gatggcttac ggcagcattg gggtaggcag cagctttctc ccctggtgtt
    13981 tattttgtgt cctgcaactg cgcgcttttc agtgcgttga tcgtcgctgg ttatgggacg
    14041 acatcgcccc gatgccagaa ctgcgccagc cgctaaaacc aggcgtcacg gggatacgtc
    14101 aggggctgcg ttcgtgctcc tgttgtacgg caatccttcc tgctgatgaa cccgtgtgcc
    14161 cgcgttgtag taccaaaggg tacgttcggc gtagaaacag cctgcagtgg acactcgcgc
    14221 tgcttgtaac gtccatcatg ctgtatcttc cggctaatat tttgcccatc atggtgacgg
    14281 atttattagg ctcgaagatg ccgtcgacga ttctcgctgg ggtcattctg ttatggagcg
    14341 aaggctctta tcccgtcgct gcggtgatct ttctggccag tattatggtg ccaacgttaa
    14401 agatgatcgc catcgcgtgg ctgtgttggg atgccaaagg gcatggcaag cgcgacagtg
    14461 aaagaatgca tttgatttat gaagttgttg agtttgtagg ccgctggtcg atgattgacg
    14521 ttttcgttat cgcggtgctc tcggcgctgg tgcgtatggg aggtttaatg agtatttatc
    14581 cggcaatggg tgcattaatg tttgctttag tcgtcataat gacaatgttt tctgctatga
    14641 cgtttgaccc gcgtttgtcg tgggatcgtc aacctgaatc agagcatgag gagtcctgac
    14701 agtatggaat ctaataatgg ggaagccaaa atccagaaag tgaagaactg gtctcccgtg
    14761 tggatatttc ctatcgtcac ggcgctcatt ggggcctggg ttctttttta tcattacagc
    14821 catcagggac tggaagtgac cctgatcacc gcgaatgcgg aaggaattga aggtggcaaa
    14881 accaccatta aaagccgtag cgttgacgtc ggcgtggttg aaagcgccac actggctgat
    14941 gatttgacgc acgttgaaat caaagtgcgg ctgaattctg gtatggaaaa attgctgcat
    15001 aaagacaccg tcttttgggt ggtgaaaccg cagattggtc gcgaagggat tagcggcctg
    15061 ggaacgctgc tgtctggagt ttatatcgaa ctgcagccag gcgcgaaagg cagcaaaatg
    15121 gataaatacg atttgctgga ctcgccaccg ttggccccgc ctgatgcgaa aggtatccgt
    15181 gtgattctcg atagcaaaaa agccgggcag ctctcgccag gagatccggt gctgttccgt
    15241 ggctatcggg taggttcggt tgaaaccagc accttcgata cgcaaaaacg caatatcagt
    15301 tatcaactgt tcatcaatgc accttatgac cgactggtga ccagcaatgt tcgcttctgg
    15361 aaagatagtg gcattgcggt tgatctgacg tcagcgggga tgcgtgtgga gatgggctca
    15421 ttgacaacgc tgcttagtgg cggtgttagc tttgatgtgc cggaaggtct ggatttaggg
    15481 cagccagtgg caccgaaaac agctttcgtt ttgtatgatg atcagaagag cattcaggat
    15541 tcgttgtaca ccgatcacat tgattatctg atgttcttta aagattcggt acgcggtctg
    15601 caaccgggag ctccggtaga attccggggt attcgcctgg gtaccgtaag caaagtgcca
    15661 ttctttgcgc cgaatatgcg tcagacattt aacgatgatt accgtattcc ggtactgatt
    15721 cgtatcgagc cagagcggct gaaaatgcag cttggcgaaa atgcggatgt tgttgagcac
    15781 cttggcgaat tgttgaaacg tggtttacgc ggatcgctga aaaccggaaa cctggtcact
    15841 ggtgcactgt atgttgatct cgatttctat ccaaatacgc ctgcaataac cggtattcgt
    15901 gaatttaatg gttatcagat tatcccgact gttagcggcg gcctggcgca aatccagcaa
    15961 cgactgatgg aagcgttgga taagatcaac aaactgccat tgaatccgat gattgaacag
    16021 gcaaccagta cgctttctga aagtcagcgc acaatgaaaa acctgcaaac gacgctggat
    16081 agcatgaaca agatcctcgc cagccagtcg atgcagcagt tgccgacgga tatgcagtca
    16141 acgttgcgtg aattgaatcg cagcatgcag ggcttccagc ctggctccgc agcctacaac
    16201 aagatggtgg cggatatgca gcgccttgat caggtgttgc gagaactgca accggtgctg
    16261 aaaacgctca atgagaagag taacgcgctg gtatttgaag cgaaggacaa aaaagatcca
    16321 gagccgaaga gggcgaaaca atgaaaaagt ggctagtgac gattgcagca ctgtggctgg
    16381 ccggatgcag ctccggcgaa attaataaaa actattacca gttacctgtg gtgcagagcg
    16441 gtacacaaag taccgccagc cagggcaatc gtctgttatg ggtagagcag gtcactgttc
    16501 ctgactatct ggcggggaat ggtgtggttt atcaaaccag tgatgtgaag tatgtgattg
    16561 ccaacaacaa cttgtgggcc agcccgttgg atcaacaatt gcgcaacacc ctggttgcca
    16621 acctgagtac gcaactgccc ggctgggtgg ttgcctccca gcctctggga agcgcccagg
    16681 acacgctcaa tgttaccgta acggagttta acggtcgcta tgatggcaag gtcattgtca
    16741 gtggtgagtg gctgttgaac caccagggac aactgatcaa acgtccgttc cgtctggaag
    16801 gagtgcaaac tcaggatggt tacgatgaga tggttaaagt actggccagt gtctggagtc
    16861 aggaagccgc ttctattgcg caagagataa agcgtctacc ttaattataa agatttgtaa
    16921 atataaccgt ctccggtatg ttgcctgagg cggttttttt gtttctaacg tgcggaaaaa
    16981 gttgttcctc ttcacatttt ttgtacaacc gacatacccg tgtagctcac aaatatgaca
    17041 gtggcgtgaa ttttgcgcat tgacggcagt tatgattcgc ggtattgctt aactgtgatt
    17101 gcacatttag taatcactgt tttcttttcc accagaaacc agtatgaggg aaacgaggca
    17161 tgaagagaca aaaacgagat cgcctggaac gggcacatca acgtggttat caggccggca
    17221 tcgccggacg ctcaaaagaa atgtgtccct atcagacgct gaatcaaagg tcacaatggc
    17281 tgggaggctg gcgagaagcc atggcggaca gggtagtaat ggcctgattc tgtctcttta
    17341 aaaagaaacc tccgcattgc ggaggtttcg ccttttgata ctctgtctga ttataatcag
    17401 aaggcagaag tatcctggaa cagaccgact ttcaggtcgc tggcggtata gatcaaacga
    17461 ccatcaacca gcacttctcc atccgccagg cccataatca gacgacggtt aacaatgcgt
    17521 ttaaagtgaa tacggtaggt cacttttttc gctgtcggca gtacctgacc agtgaatttc
    17581 acttcgccaa cgcccagtgc gcggccttta ccttcgccgc ccagccagcc gaggtagaac
    17641 cctaccagct gccacattgc gtccaggccc aggcatcccg gcataaccgg atcgccaata
    17701 aagtggcatc cgaagaacca cagatccgga ttgatatcca gttctgcttc aacataccct
    17761 ttgtcgaagt taccacccgt ttcggtcatt ttgaccacac ggtccatcat cagcatattc
    17821 ggtgctggca attgcgggcc tttagcgcca aacagttcac cgcgaccaga ggcaagaagg
    17881 tcttcttttg tataggattc gcgtttatct accatgttct ctgtaagcct tattttattg
    17941 aagcacgcag gatagctaac acgtgtacgc tgaacaagtc cgatcagttc ggaataaacc
    18001 agttcagcca acgtaatggc catggaaaac ggtgacgtcc ttcctgttgc gatgcttgcg
    18061 cgatacgttc ctggatggtt tgcatcagcg tcgtttggcc ttcgccatcc cacaccagat
    18121 ttaataataa cggcagtgcg tcagtcacat cgtctactgc ccagatggtg aatttgcctt
    18181 cttctaccgc tttcactagt tcactgtgaa gacttaaatg gcgaacgtta gctgtgggga
    18241 taatgacacc ttgtttcccg gttaactcac gttgctggca aatagcaaag aagccttcga
    18301 ttttctcatt taaaccaccg accggctggg cgcgaccgaa ctgatcgact gaacctgtga
    18361 tagcgatact ctgattcacc ggcacatcgg cgagggcgct tatcagggcg cagagttcag
    18421 ccatcgaggc actatctcca tcaacttcac tgtatgactg ctcaaatgtc agcgatgctg
    18481 agaaggggat ctgttgctca agctgtagtt ccgacatcag gaacgcttgc atgatcatca
    18541 tccctttcgc atggatattg ccgccaagct ccgctttgcg ttcgatgtcg gtgaattcac
    18601 catcgccaat atgcacaacg cagctaatgc gagaaggttc gccaaaagcg cgtggatgac
    18661 ccggaaattc aatgaccgaa agggcgttaa tttgcccgat gcgttcgcct tcggtttcaa
    18721 tcaggatttg ctcctgaagg atctcatcct gcatacgttc agcgaggaaa ccttcgcgcc
    18781 attcacgctg ctgcagcatt aagtttagct gctcgccgga gaaggtgtcg ccatcacaca
    18841 gggaggcgac ctctttacac tggcggagga tccactgcgg gctaagcgga agtgtttctt
    18901 gttcaccggt gtagcgtgct gcttcgcgga taagtaccgg ccaggcatcc gctcccggtg
    18961 caggtaagtg attatgtctg gcggtaaatg tcacccagcg acaccactgg gttactgact
    19021 ccgcatcgac aatctgcaga gtatcttcaa attcgctata aatagcctgc tctgaaagct
    19081 ctggctccat ctcctggaaa tcagccaatg attcgcgttc gcctaccaga atgactttca
    19141 gcttcaatgg catcgaaggc acagagacgg ggagagggcg cgactcatca aacgcaaccc
    19201 agtcaaaacg ctcgcggtta acgatatttt tcagccgcat ccacagcaga ggttgcgcca
    19261 gcagtgtacg caaagagatg atgagaatac cgccatttgc ctgatgcacc agaccaggct
    19321 gcagggtaat gtcgccatta aactggcgca ggcagccaaa gagttgctcc gcttctaccc
    19381 agtcggcagc gacaacttgc gttaaagtcg caaaattatc atctgcactc actgcgtgac
    19441 gtaagcggat ggagtggccg gaaacctcat agtgaccgcc caccagttgg cctgcatcgc
    19501 tttgtagcgt acgcgcggca ttggcaataa gattcagata ctcagactct tccggggcct
    19561 tcgccagcat aaaagaggag gatgctcgcg tatgcagcaa ctgctccagc gcaaattgca
    19621 gccgcggttg agtatcactg aataaaggat cgttttcgtc aatcaaatgt ggctgagcaa
    19681 atatttcctg atagctatcg gtatcaggaa ccaggtcacg ccatgcaagt ttcgtaatgg
    19741 tcaaagttga tgttttttag tctgttgtca aagccgcgat tataccgtaa ccggcactac
    19801 agcacacgta gaaagcaccg acaatactcc aggcatgggc gttaaagctc acaggatgga
    19861 gattcttttc ttcactggcc taaaaagctg atattctgta aagagttaca cggtaacatt
    19921 gagatcgcta tgaaatatca acaacttgaa aatcttgaaa gcggttggaa atggaagtat
    19981 ctggtgaaga agcatcgcga aggggagtta atcacccgtt acatagaagc cagtgccgcc
    20041 caggaagccg tcgatgtgtt gctctcgctg gaaaatgaac ccgtgctggt aaatggctgg
    20101 attgacaagc atatgaatcc ggaactggtc aatcgcatga agcaaaccat tcgggcaaga
    20161 cgaaagcgcc attttaatgc ggaacaccag catacgcgca aaaaatcgat cgatctggaa
    20221 tttattgtct ggcaacgtct ggctggtctt gcgcagcgtc gcggtaaaac gctttctgaa
    20281 acgattgttc agctgattga agatgcggaa aacaaagaga aatacgcgaa taaaatgtct
    20341 tctctgaagc aggatctgca ggcattgctg ggtaaggaat aactgacgaa agtcagttca
    20401 atttactaaa ggcaaaaaaa accccgcagc agcggggttt ttctaccaga cgagaactta
    20461 agcctgcggc tgagttacaa cgtctttgat acctttaact tcgatctcta cgcgacgatc
    20521 cggagccagg cagtcgatca gtgcagcacg ctgtttcacg ttgtcacagg tgttgccagt
    20581 aaccgggttg gattcgccca taccacgtgc ggagatcttg tctgccggga tacctttgga
    20641 gatcaggtaa tcaacaacag actgagcacg gcgctcggac agaccctggt tgtaagcgtc
    20701 agaaccgatg cggtcggtgt aacccagaac aactacggaa ccgtctttcg gatccaggtt
    20761 gctcagctgg ctgtacagct gatccagagc agcctgacct tccggtttca gggttgcttt
    20821 gttgaagttg aacagaacgt cagacttcag agtgaagtgc ttggtctgta cttccggtgc
    20881 cggagctgga gccggagcaa ctactggagc tgcttcgccc tgaccgaaac ggtaggaaac
    20941 ccccaggctc agcatgccgt tgtccggacg agtgccgatg gtgtgtgcgt caccgatgtt
    21001 gttggtccac tggtattcca gacgggtagc gatttcagga gtgatcgcgt actcaacacc
    21061 gccagcgaag accggagaaa cgccggtgtc gtggttttta ccataaacgt tggatttagt
    21121 gtctgcacgc cataccatac cacccagacg agtgtagatg tccaggtcgt cagtgattgg
    21181 gtaacccagt ttagcggtca gttgaacgcc ctgagctttg tatgcaccgt tttcaacgct
    21241 gcctttgtac ggcatacgac ctaaccagtc gtaacccatt tcaaagccaa catacgggtt
    21301 aacctggtaa ccaccaaaag caccagcgcc cagttggttt tcatgggtcg ggccattgtt
    21361 gttgatgaaa ccagtgtcat ggtactggga ccagcccagt ttagcaccag tgtaccaggt
    21421 gttatctttc ggagcggcct gcgctacggt agcgaaacca gccagtgcca ctgcaatcgc
    21481 gatagctgtc tttttcattt tttgcgcctc gttatcatcc aaaatacgcc atgaatatct
    21541 ccaacgagat aacacggtta aatccttcac cgggggatct gctcaattaa ctctaccgat
    21601 atcttcggct tatgccgagc acccctggcg atgtaaagtc tacaacgtag ttgaaaactt
    21661 acaagtgtga actccgtcag gcatatgaaa aaaaagtctt gtataaggta tgtttaatct
    21721 tttttgtcag cgacaattta cagaagaaaa tcgcggaaac cgcttcagac aagcctccgc
    21781 aaggaaaatt agtcacgact gaaagcattg gctgggcgac aaaaaaagtt ccaggattaa
    21841 tcctaaattt acttaatgat acaaattaga gtgaattttt agcccggaaa gttgtctcgt
    21901 ggcgtgagag gatgcgctta ccggacgcat aataaacccc atagcgttac cttcatttgc
    21961 cgcatcaaca agttcagcat gctcttcttc agtcaaatca tctgccaacc aaccgatcac
    22021 cacactgtaa ttgcccgtgc gtaaagcgcg aaccattgac tccacagtgt ggcaagggga
    22081 gagctggcta atctgcatta ctttcgttaa gggtagccca gatgcctgaa cccattcccg
    22141 actcagtttt tgttgcggtg ttaaccagag ttgccagcgc gattgctgac cgagttgctg
    22201 taacaatggc aacagtagaa gttgcgtcat catgggctga tcttcgcgat agacaacttc
    22261 actgataagc ccggctgtag tgttttccgt agagacacgc gcaattttac ttgctgcgga
    22321 tgagaacgac gaagaacgat gtgcatagcc tgaagtgtac ataatcaatc cagcccctgt
    22381 gagttactgt atggatgtac agtacatcca gtgacaacaa agatcaaccc tattttcgga
    22441 aagagcctcg caaattttgt cgttggtgac gggaaaacat aaattaatct tgccccttaa
    22501 gaataagttg cctattttcg tagttaacgg atccgttaat gtgaatcatt cttttatgtt
    22561 atgattttaa aaggaatttt atgaaaagcc tctcctataa gcggatctat aaatcacaag
    22621 aatacctggc aacgttgggc acaattgaat accgatcatt gtttggcagt tacagcctga
    22681 ccgttgacga cacggtgttt gcgatggttt ctgatggtga gttgtatctt cgggcttgtg
    22741 agcaaagtgc acagtactgt gtaaaacatc cgcctgtctg gctgacatat aaaaagtgtg
    22801 gccgatccgt taccctcaat tactatcggg ttgatgaaag tctatggcga aatcaactga
    22861 agctggtgcg tctgtcgaaa tattctctcg atgcagcgct gaaagagaaa agcacgcgca
    22921 atacccggga aagactgaaa gatttgccca atatgtcttt tcatctggaa gcgattctcg
    22981 gggaggtggg gattaaggat gtacgggcgt tacgtatact tggggcaaaa atgtgttggt
    23041 tgcgactgcg gcagcaaaac agtctggtga cagaaaagat tctgtttatg cttgaaggtg
    23101 ccattatcgg cattcatgaa gctgcgctcc cggtggcacg ccgccaggag cttgcagaat
    23161 gggctgactc tcttacgccg aaacaggagt ttcctgcgga acttgagtaa tctggcgttg
    23221 cagacgacca atctcaggca gtaatgcaat caataatcca acttgttgta cgaccagagg
    23281 ttctttgctg tctgcccgtg gttcaagttg ctgcatccgc tgtttcaggc tagctaatgc
    23341 ctcattgacg cgttcttcat cagcaggttg atgatgtaac gcgtcatcaa catagcaaac
    23401 tgcgtcatca agaaacgcca gaatttcagg attagttaac tgctcccggt gagcaccgag
    23461 ggctgagata tagctggtaa acgtatggtt aaggcacagc aaccgaaacg cggcttcgcg
    23521 aatttgcggg gtaacgttcg gctcgctgga catatttgat accaccgacg ccagctcagc
    23581 atcacggttg tgtgcatcgc ggcgggcaat acgatacgcc agacggttat cacgcccctg
    23641 atggtattgc tccagtatgg catcgagata ccgacagttg gcctctgtgg cgcgttcgag
    23701 catgcgcggc agattgcgaa actgccagtc aggccagatg tagctcactg ccgcccacgc
    23761 aatggcacaa ccaatcagcg tatcgattac gcgaggtaac gctacttcaa aaccttcacc
    23821 cagtaagtta aaacacagta gcaccaaaag tgtgatgaac atcgttgcat gagcgtattg
    23881 cacgttacgg aaggcaaaaa agagcacgcc ggtaataacc agcagcacca gctgcccttc
    23941 cagtgatggc acaaaccaca gcacaggaat gccaatggcg atacctacca gcgtaccaat
    24001 aatccttaac ttcaggcggt ggcgcgtggc gttatagttt ggctggcaga caaacaaact
    24061 tgtcagcaag atccaatacc cgtgatgcat tccggttatc tgaatgatgg cgtagccgaa
    24121 gcacaacacc agcgacattc ttaccgcatg acggaagagg gcggattccg gcgtgaagtg
    24181 acggctaaga cgcagccaga tatcactcaa cccgtgcggg ctgtcatcag cgagctcatt
    24241 ttcgtcatta ttatggggta gtgcctgggc ctgttctgat tcaattgttg ccagttgggc
    24301 atcaatggcg cgtaaattgt tcagcaaaaa tcccagtgtt ttgagtaaat cggcgggtgc
    24361 gccgttatcg cgcatccgct ccagcgcagc atcaatatgc gtaaaagcgc gctcaaaatg
    24421 cggatcatgt tgataaggct gacgcaacaa aatacagcgt gacagttgct ggcacgcctg
    24481 gccctgcatc gacatcagcc gctgaaaacg gaacagcacg tcgctgtggc gaaaatgttc
    24541 acgcaatgtt tgatactgaa tatgagaaga gctggcacgc tcgtgaatat cctgtgcgac
    24601 aaagtaataa tgcagcgtgc gacgcgttcc ccgttgacca cgatcgccac gtaagcgggt
    24661 cagcagcgag agtttcgtct gattcaatgt cgccatcagc agaccgttgg cgagagccaa
    24721 atcgtacagc ggtgcctggc tttcatcttc aatatcagga tcaaacatgc gcgacttgag
    24781 ctcaagataa cgcgccagtt gttcatagca acgcgccagg ttgtcctgca gcgggcggac
    24841 cgggaacagc agatgaccaa taagtgtcag gacgttgtac cagacggcac cggccagcag
    24901 atacatcggc tgctgatacc agtgctcata cagtgatgtt cccaacatag tgtaaatggc
    24961 gatcagcaat gcaccgaagg caattgttgc atagcgttga cccagaccgc cgagcaaaat
    25021 gaagccgctg gtagagagcg ttaagccaat cgcaaatagc cagggccagg gaaacagcaa
    25081 ttctactgag gccgaggcga taaaaaagca gaacagcgta atgatgaggt tacgcaaacg
    25141 tcccgccagt cggtcatcga gatcggtcag cgccgctgcc accatcccca gcgttagcgg
    25201 aatcgtcagt tttacatcac ccagccacca cggaaacgct gtggttccac aaagcgcaat
    25261 aaaaatacgc gcgtaataca gccaggcgct gttccaggta tagcgtttga gcaaaggact
    25321 tagcataaag gccatattag tggattattc aaaacgacga cgtgcattgg cttcacgcgc
    25381 agcttgcgct gtttctaccg ataccacgcg acgaccaacc ggccatagtg caatagcggc
    25441 aattttaaag ttcgcaatgc cgacaggaat gccaatgatt gaaatacatt gtgcgatgcc
    25501 cgttgcaatg tgcatcaggc ataaccacca gccaaagaaa atcagccaga aaatattcaa
    25561 taccgtaccg ccagtattca gcagcacatt tttgccagcc gggttcagtt catcgacatg
    25621 aatagcttca ttgccataag gcaccagaga tagtttagtg atctcccagc aggatcgtgt
    25681 cagcggtaag gtaaaaatca gcacaatact gaccagagtc gccaacagcc agcccagagt
    25741 ggtggcaaat ccgccaagca caaagttcag aatgttcaaa acggtacgca taaaacctcg
    25801 ctttactgtg gttttcagta atgggcggca attgtaacgt ttttttgggc tggagcacgt
    25861 tttctctgac ggttacactg ataagaaata atttcgtgtg gatctacaga gtcatggaac
    25921 tgaaagcgac aacgcttgga aaacgtctgg cacagcaccc ttacgatcgg gcggtgatcc
    25981 tcaatgccgg gattaaagtc tccggcgatc gccacgaata ccttattcct ttcaatcaat
    26041 tactggcgat tcactgtaag cgcggtctgg tatggggcga gctggaattt gtactgccgg
    26101 acgaaaaagt ggtgcgtctg cacggcaccg aatggggcga gacgcagcgt ttttaccatc
    26161 atcttgatgc tcactggcgg cggtggagtg gcgagatgag cgaaattgcg tctggtgttt
    26221 tacgccagca actggatttg attgccacgc gcactggaga aaataaatgg ctgacgcgtg
    26281 agcaaacctc tggcgttcag caacaaatcc gccaggcttt gtcggcgttg ccgttgccgg
    26341 ttaaccgact ggaagaattc gataactgcc gtgaggcgtg gcgtaaatgt caggcctggt
    26401 tgaaagatat tgaaagcgct cggttgcagc ataaccaggc gtataccgaa gccatgctta
    26461 ccgagtatgc ggattttttc cgccaggtcg agtcttcacc gctgaatccg gcgcaggccc
    26521 gggcagtcgt taatggcgag cattctctgt tagtgctggc aggtgcagga agcggaaaaa
    26581 cgtcggtgct ggtggcccgt gcaggctggt tgctggcgcg tggtgaagcg tcccctgagc
    26641 aaattttatt gctggcgttt ggtcgcaaag ccgctgaaga gatggacgag cggattcgcg
    26701 aacggctaca taccgaagac attaccgcac gcacgtttca tgcgctggcg ctgcatatta
    26761 ttcagcaggg cagcaaaaaa gttccgatag tcagcaaact ggaaaatgat accgctgccc
    26821 gtcatgaact ctttattgct gagtggcgca agcaatgcag cgaaaagaaa gcgcaggcga
    26881 agggctggcg gcaatggctg acggaagaaa tgcagtggtc agtgccagaa ggtaacttct
    26941 gggatgatga aaaattacag cgtcgccttg cctctcgcct cgatcgctgg gtaagtctga
    27001 tgcggatgca cggtggtgca caggcagaaa tgattgccag tgcacccgaa gagattcgcg
    27061 atctgttcag taaacgtatc aagttgatgg ccccgttatt aaaagcctgg aaaggtgcgc
    27121 tgaaggcaga aaacgctgtc gatttttcgg gccttattca tcaggcgatt gtgattctgg
    27181 agaaaggtcg ctttatcagc ccgtggaagc atattctggt tgatgaattt caggatatct
    27241 cgccgcagcg ggcagcgttg ttagcggcat tacgcaagca aaacagtcag acgacgttgt
    27301 tcgctgttgg tgatgactgg caggcgattt accgattcag cggtgcgcaa atgtcgctca
    27361 ccaccgcttt ccatgaaaac tttggtgaag gcgaacgttg tgatttagac acgacttacc
    27421 gttttaacag tcgtatcggt aaggtggcaa accggtttat tcagcagaac ccaggccagc
    27481 tgaaaaagcc gctaaacagc ttaaccaatg gagacaaaaa agccgtcacg ttattggatg
    27541 agagtcaact tgacgctttg ctggataagc tctctggtta tgccaaaccg gaagagcgca
    27601 ttctgatcct ggcgcgttac catcacatga ggcctgccag cctggaaaaa gcggcaacac
    27661 gctggccgaa gttgcaaatc gactttatga ccattcatgc cagcaaaggg caacaggcgg
    27721 attacgtcat catcgttggc ttgcaggagg gaagtgatgg ttttccggct gcggcgcggg
    27781 agtcgattat ggaagaggcg ctactgccac cggttgagga tttcccggac gctgaagaac
    27841 ggcggttaat gtacgtggcg ctgacccggg cacgccatcg ggtatgggca ctgtttaaca
    27901 aagagaatcc ctctcccttt gtggaaatac tgaaaaatct ggatgtgccg gtggcgagaa
    27961 aaccgtaaga aacaggtggc gtttgccacc tgtgcaatat tacttcagac ggtccgcgag
    28021 ataacgctga taatcgggga ttagaatatc gaccgcgtcg ttgaaatgcg gcgactggat
    28081 tatgaagtct gccgttgcca cgttggtggc gaccggaatg ttccataccg tcgccagacg
    28141 cagcaaggct ttcacgtcag gatcgtgcgg cacggcattt agtggatccc agaagaaaat
    28201 caatacatca attttccctt ctgagatcaa tgcgccaacc tgctggtcac cccccattgg
    28261 gccactcaac atcgcgttga cgttcatgcc ggtcgcgcgg gaaattaagt taccggtagt
    28321 gcctgttgca tacagtacgt gttgttccag taacggttga tgccgttcca cccagctcat
    28381 cagcatttgt ttgcagtgat cgtgtgccac cagcgcaata tgtttccgcg caggtaaagt
    28441 gcgagtcgtc agttccataa tgtacatccg tagttaactt tcctacagat tactgtaagc
    28501 acttatcgct gcaagataaa gaccgaaaaa gcctgcgcac aggcacaaaa atctcaggaa
    28561 gatggttgtt tttccgccca ctgcaggaaa gtatttcgcg tttgtgggtc agccagttta
    28621 aaccaatact tcagccgttg ttctgtgagc acctgagact gcggtggaat agcatccagc
    28681 tcggaaacac ccgaaagcag cgtactgtca tctgccatca tcgtggcaaa ttgcggtagt
    28741 gaagcgcgtt tcgcggattt gttatagcgt tcaacctcga cctcataatc aatcgttttt
    28801 gccgttgagg taatggcgag aatatccagc tttaccggaa tcggcgtcgc atcgccatcc
    28861 aacaattcaa ggcgcggcgc ggcatcaaaa tggttagctt cccgctcatt ctccaggcga
    28921 ggcaggcgaa aattgacctg gttgatgagc tgggtattaa aactgaccac cagcggtggg
    28981 gagatataca gccgttcttc actattggaa agatgaatcg tcttctcaac gcgaaacact
    29041 aactgatgcg gtccattatc cagttcaatg ctgtccgcac cgcgcagcag agagctggaa
    29101 accttcttgc cgtcgagtac cagaagatcg acatcggttg aaagccgcag cgtggtggca
    29161 aaaacggata ccggcaaaca taatgcaatt aaggtggtca cgatgccggt tttcataggt
    29221 agctcctgtc aaaagaccgc ccgaataata atgtgtcaaa atttttcttt aatcatactt
    29281 actaacatat agacatattt cccgcttttg ctctcattca ttcgtattag ctgcatggtt
    29341 ggcatgtcgg ctttgtcgta cactttgtta aacagtctgg aggaaataat gaaagaaacc
    29401 gatattgcag gcattttaac gtccactcac accattgcgc tggtgggggc aagcgacaaa
    29461 cccgatcgtc ccagctatcg cgtgatgaaa tatctactcg accaggggta tcacgtcatt
    29521 ccggtttcgc caaaagttgc cggcaaaacg ctgctgggcc agcaggggta tggcacgctg
    29581 gcagacgttc cggaaaaggt cgatatggtc gatgttttcc gtaattcaga agcggcgtgg
    29641 ggcgtggcac aggaagctat tgccattggc gcgaaaaccc tctggatgca actgggggtg
    29701 attaatgagc aagcggcagt actggcacgg gatgccgggt tgaatgtggt gatggatcgc
    29761 tgcccggcta ttgagatccc tcgcctgggc ctggccaaat aaaaaatccc cggaaggcaa
    29821 aaaccttccg gggattcgtt cagggatcag ttacgcagac gcggggcctg gagttgtttg
    29881 cggatggtct gcgccagctc atccatggat ggctgttcag gatgttcatc ctgcaattcg
    29941 ctacttagct gggcttcagc caggtaggta tgaaccggta ggccgttatc gtcctccatc
    30001 actacgtgat accacggagc ggcgcgaagc tcgtcattta ccgccagctc atcaggcgac
    30061 ggttcagaga gcgaataaac cgggtcgata tccacgacca ctccgagata acctaacagg
    30121 gaatggcgga cctgctggcc gataccgaat ttgctggcaa tcatagtcac ctcccgggaa
    30181 atctttacct ttgatataag ggtaaaattc cacatttcaa gttacatgac gcgacaggca
    30241 aaccctttca gatatagccc ttccggatag gtagcgatca ccggatgatc ggctgcctga
    30301 cggaactgct ctataaattg tacatcacgg ccggcatcaa ttgcggcatc cgcgatgatt
    30361 ttctgaaata aatcgctggt catcagaccg gaacaggaga aagtcaggag aataccgcct
    30421 tcattcagca actgaatcgc cagcatgttg atatctttat agccacgaca cgcgcccatc
    30481 aactggcttt tattctcaac aaacttcggc gggtccatca cgataacgtc aaatttttca
    30541 ccgcgatcgc gataagtacg cagcaattta aagacatcat cacggacaaa ctcagccttg
    30601 ctcagatcca gtttgttcag ctcaacgttc tgccgtgcaa tatccagcgc ttcctaggag
    30661 gtatcaacgc tgacaacctg gctgcaaccg cccatcagtg ccgatacggc gaaaccaccg
    30721 gtataggaga aacagttcag cacacgttta ttttcaacgt agcggcgggt agccaggcgg
    30781 ctgtctcgct ggtccaggta gtagcccgtt ttgtgtccgt gctgaatatc caccagcagc
    30841 ttcatgccgt gttcttcaat cggcagcagg gcaggcggca actcgccggt gacgaggccc
    30901 tgggtcagct ccatcccttc ttttttacgt accgcaacat cgctgcgatc gtaaatcgca
    30961 cattccgggt acagcgtttg cagggcacta attaatgccg cgcgctgata ttctgcccca
    31021 gcactcagca gttgcagcac cagaaaatta ccgaaacgat cgatagtaat acccggcagg
    31081 ccatcagatt ctccggcgat taaacgatag ctgtcgaggc catctttttg cgccagtcag
    31141 tcacgccatt tttgtgcttg ttgcaaacgg cgggaaaaaa aagcaatgtc gatagactca
    31201 gacgggtcaa acgtccagac gcgcgcccgg atttgcgaag ctggcgaata agcgccgcgt
    31261 gctaaccatt ttccctgatg atcaacaata tcgatggttt caccgaggct ggctttacct
    31321 tccatgcggg caacggcccc ggaaaagacc cacggatggc gacgaagtaa tgatttttcg
    31381 cgccctttgg ctaacactaa acgtacactc ataatttact tttctgtcga tgcaaagaaa
    31441 tgggcgtcat tgtccggagt tttccatgga tttgcaacgc gtccattaag gataaggaga
    31501 gcagcaatgt cgaaagtctg cataattgcc tgggtttacg ggagagttca gggcgtagga
    31561 tttcgctaca ccacacagta cgaagcgaaa agactgggtt taaccgggta cgccaaaaat
    31621 cttgacgatg gcagcgtgga agtggttgcc tgcggtgaag aagggcaggt ggaaaaatta
    31681 atgcagtggt taaagagtgg cggcccgcgt tctgcgcgtg tagaacgggt gctcagcgag
    31741 ccgcatcatc cctcggggga attaaccgat tttcgtatcc gttaaataca ttttaccggt
    31801 tttggcaggc cagcaatttt ggtggcttgc ttagccggac ctttcggaaa cagtcggtat
    31861 aaatagcggc tgttaccttt ttcttcgcca aatttattcg ccatcgcttt taccagcata
    31921 cgaatcgccg gagaagtatt gaattccaga tagaaatcac gcacaaaacg caccacttcc
    31981 cagtgttccg gtgacagcga aatcccttcg ttctctgcaa tcaccactgc cagcggctca
    32041 ctccactggc tgctttcttt gagatagcct tcggtatccg tttctatctc tttaccttcg
    32101 aagatcagca taattattac taccttaatc agactggcgg cagtgtaaca aaaaacaaag
    32161 ccccgcataa agcgaggcta tgaaagtgtt agcgggtgag attaatcgcg gctggcgaag
    32221 cccagaatgc tcagcaggct gacgaagatg ttgtacagcg aaacatacag gctaaccgtg
    32281 gcacgaatat agttcgtttc accgccatga acgatgttgc tggtttcaaa caaaatagcg
    32341 ccagaggaga tcagaatgaa gaccgcgctg atcgccagat gcagagcagg cagctgcagg
    32401 aagatattag caaccatacc aatcagcacc accacaatac ccgccatcag cataccgccg
    32461 aggaacgaca tatctttgcg ggtggtcagc acatatgcag agcagcagaa gaacactaac
    32521 gccgttccgc ccagtgccat agcgattacg tcacccattc cggcagacag ataggtgttc
    32581 agaataggtc cgaggatata acccagaaaa ccggtaaagg cgaatgcgga gataatcccg
    32641 gtcggcttat tcgccgtttt ataggtcagg aacatcaaac catacatacc caccagcgtc
    32701 agaatcagac ccggagatgg cagcatcagc accgtactgg cagttgcggt aatcgccgaa
    32761 aaggccagcg tcaggctcag cagaaaatag gtattacgca gcaccttatg ggtgctaagc
    32821 agtgatgtac ggtcatgtga agaactaaca atacgatcca ttgagtcact ctctatgaca
    32881 gatgtaatta attaagcagc ataatgataa tgcgtaaggg cacccagaag ttttacccat
    32941 ctttacgcat ttgatctgga acaggtttaa cagcggatta tcaggtcatt aagcaaatat
    33001 aacgccctga gaatttcgac aggcaaaaga aaaaggggtt agcatttagc taacccctta
    33061 tcttatttgg cggaagcgca gagattcgaa ctctggaacc ctttcgggtc gccggttttc
    33121 aagaccggtg ccttcaaccg ctcggccaca cttccggaat gacgcgcact ataaacatcc
    33181 cgatgcggcg tgtaaacccc taatttgttt gtttgcctga aaaacagcca aaagtgcatt
    33241 gatagcgtga aataacagca gattgatcat ttcatcacca tgaattcctt ctcttttact
    33301 cgtttagcaa ccggctaaac atccccaccg cccggcaaaa agaaaaatag gtccattttt
    33361 atcgctaaaa gataaatcca cacagtttgt attgttttgt gcaaaagttt cactacgctt
    33421 tattaacaat actttctggc gacgtgcgcc agtgcagaag gatgagcttt cgttttcagc
    33481 atctcacgtg aagcgatggt ttgccttgct acagggacgt cgcttgccga ccataagcgc
    33541 ccggtgtcct gccggtgtcg caaggagaag agacgtgcga tatgaataac gaggaaacat
    33601 tttaccaggc catgcggcgt cagggcgtta cccggcgcag ctttctcaaa tattgtagtc
    33661 tggctgccac gtcgctggga ttaggcgcgg gaatggcacc aaagattgcc tgggcgctgg
    33721 agaacaaacc ccgcattccg gtggtatgga tccacggtct ggaatgcacc tgctgtaccg
    33781 aatcttttat ccgctccgct cacccactgg cgaaggacgt catcctttcc ctgatttccc
    33841 tcgattatga cgatactttg atggctgccg ccggaaccca ggcggaagaa gtcttcgaag
    33901 acatcatcac gcaatacaat ggcaaatata tcctcgcagt agaaggtaat ccgccgctgg
    33961 gcgagcaggg gatgttctgt atcagcagcg gtcgaccgtt tattgagaaa ctcaaacgtg
    34021 ccgctgccgg agccagcgcg attatcgcct ggggaacctg cgcgtcctgg ggctgcgtgc
    34081 aggccgcgtg gcccaatccg acgcaggcaa cgcctatcga caaagtcatc accgacaaac
    34141 ccattatcaa agtacctggc tgcccgccga tcccggatgt gatgagcgcc atcattactt
    34201 acatggtgac ctttgatcgc ttgccagatg tcgacagaat gggccgtccg ctgatgttct
    34261 atggtcagcg aatccacgat aaatgctatc gccgcgccca cttcgacgcc ggagagttcg
    34321 tccagagttg ggatgatgac gctgcccgca aaggttactg cctgtacaaa atgggctgca
    34381 aagggcctac cacctataac gcctgttcct ccacacgctg gaatgatggc gtttctttcc
    34441 caatccagtc tggtcacggc tgcctgggct gtgcggaaaa tggtttctgg gattgcggtt
    34501 cgttctacag ccgcgtggtc gatattccgc aaatgggtac tcattccacc gccgataccg
    34561 tcggtttaac cgcgcttggt gtggtggcag cggctgttgg tgtgcacgca gtcgccagcg
    34621 ccgttgacca gcgcagacgt cataaccagc aacctacaga aaccgaacat cagccaggca
    34681 atgaggataa acaggcatga gcactcagta cgaaactcag ggatacacca tcaataatgc
    34741 cggacgccgc ctggtggtcg acccgattac gcgcatcgaa ggccacatgc gctgcgaagt
    34801 gaatattaac gatcagaatg tgatcaccaa tgccgtctcc tgcggcacca tgtttcgcgg
    34861 gctggagatc atcctacaag ggcgcgaccc gcgcgatgcg tgggcgttcg ttgaacgtat
    34921 ctgcggcgtc tgtactggcg tacacgccct ggcttcggtt tacgccatcg aagatgctat
    34981 cggtattaaa gtgccggaca acgccaatat catccgcaac attatgctgg caacgctctg
    35041 gtgccacgat catctggtgc acttctatca gcttgccggg atggactgga tcgatgtgtt
    35101 agatgcgctg aaagccgacc cgcggaaaac ctccgaactg gcgcaaagtc tctcctcttg
    35161 gccgaaatca tcccctggct atttcttcga cgtacaaaac cgcctgaaaa aatttgttga
    35221 aggcgggcag ttggggatct tccgcaatgg ctactggggg cacccgcagt acaaactgcc
    35281 gccagaagct aacctgatgg gctttgccca ctatctcgaa gctctcgatt tccagcgtga
    35341 aattgtcaaa atccacgcgg tctttggcgg taaaaacccg catccaaact ggattgtcgg
    35401 cgggatgcct tgcgccatta acattgacga aagcggcgcg gtcggggcgg tcaatatgga
    35461 acgcctgaac ctggtgcagt cgattatcac ccgcacggcg gacttcatta acaacgtgat
    35521 gatccccgac gccttagcca tcggtcagtt caacaaaccg tggagcgaaa tcggcacggg
    35581 tctttctgat aaatgtgtcc tcagctacgg tgcgttcccg gatattgcca acgactttgg
    35641 tgagaaaagt ctgctgatgc ctggcggcgc ggtgattaac ggcgacttca acaatgtgct
    35701 gccagtggat ttggttgatc cgcagcaggt gcaggagttt gtcgaccacg cctggtatcg
    35761 ttatcccaac gatcaggtcg ggcgtcatcc attcgatggc attaccgacc cgtggtacaa
    35821 ccccggcgat gtcaaaggca gcgataccaa cattcagcag ctgaatgaac aggaacgcta
    35881 ctcgtggatc aaagcgccgc gctggcgcgg taacgcgatg gaagtggggc cgctggcacg
    35941 cacgttaatc gcttatcaca aaggcgatgc tgcgaccgtt gagtcggtcg atcgcatgat
    36001 gtcggcgctg aacctgccgc tttccggtat ccagtcaacg ttgggccgca ttttgtgccg
    36061 cgcgcacgaa gcacagtggg ccgcaggtaa gttgcaatat ttcttcaaca agctgatgac
    36121 taacctgaaa aacggcaatc ttgccaccgc ctccacggaa aaatgggaac ctgcaacctg
    36181 gccgacagag tgccgtggcg tcggctttac cgaagcgccg cgcggggcgt taggccactg
    36241 ggccgccatt cgcgatggca agattgatct ctaccagtgc gtggtgccga ccacctggaa
    36301 cgccagcccg cgcgatccca aagggcagat tggcgcttat gaagcggcgc tgatgaacac
    36361 caaaatggcg atccccgagc aaccgctgga gatcctgcgt actctgcaca gctttgaccc
    36421 gtgcctcgcc tgttcaacac acgtgctggg cgacgacggt agcgagctga tctccgtgca
    36481 ggtgcgttaa cagcgaagga gaatcatcat gcaacagaaa agcgacaacg ttgtcagcca
    36541 ctatgtcttt gaagcgccag tccgcatctg gcactggctg acggtgttat gcatggcggt
    36601 gttgatggtc accggatact ttatcggcaa gccgctacct tccgtcagcg gcgaggcgac
    36661 gtatctgttc tatatgggct acatcaggtt aattcacttc agcgccggga tggtttttac
    36721 cgtggtcttg ctgatgcgga tctactgggc ttttgttggc aatcgatact cccgcgagct
    36781 gtttatcgtg ccggtatggc gtaaaagctg gtggcagggc gtgtggtatg aaatccgctg
    36841 gtatctgttc tggcaaaacg tccgagtgcc gatataggcc ataaccccat cgcccaggcg
    36901 gcgatgttcg gctatttcct gatgtcggtc tttatgatca tcaccggctt tgcgctgtac
    36961 agcgaacaca gccagtacgc tatttttgcg ccgttccgtt atgtggtgga atttttctac
    37021 tggacgggag gcaactcaat ggacattcac agctggcatc ggctggggat gtggctgatt
    37081 ggcgcgtttg tgatcggtca tgtctatatg gcgctgcgtg aagacatcat gtccgacgac
    37141 acggtgatct ccaccatggt caacggctac cgtagccaca aatttggcaa aataagtaac
    37201 aaggagcgtt catgagcgag caacgcgttg tggtaatggg gctgggcaac ctgctgtggg
    37261 ccgatgaagg tttcggcgtg cgggtggcgg aacggttgta tgcccattac cactggcccg
    37321 agtatgtgga gattgtcgat ggcggtactc agggactgaa cttgctgggg tatgtcgaaa
    37381 gcgccagcca tctgttgatt ctcgatgcca ttgactacgg gctggaacct ggaacgctgc
    37441 gaacctatgc cggagaacgt attccggctt atctcagcgc gaagaaaatg agcctgcacc
    37501 agaacagttt ctccgaagtg ctggcgctgg cagatatccg cggacatctg ccagcacata
    37561 ttgccctcgt cggtctgcaa cccgcaatgc tcgacgacta cggcggcagc ctgagcgaac
    37621 tggcacggga gcaactgccc gcagcggaac aggcggcgct ggcgcagctg gctgcatggg
    37681 gaattgtgcc gcaaccggct aatgaatcgc gctgtctcaa ttatgactgt ctgtcgatgg
    37741 aaaattacga aggcgttcgc ttgcgccagt acaggatgac actggaggag cagggatgag
    37801 caacgacacg ccatttgatg cgttgtggca acgaatgctg gcgcgcggct ggacgccggt
    37861 tagtgaattc cgtcttgacg actggcttac gcaagcgcca gacggcgtgg tgttattaag
    37921 cagtgacccg aaacgcacgc cagaggtcag cgataatccg gtaatgattg gcgaattact
    37981 gcgcgagttt cccggctaca catggcaggt ggcgattgct gaccttgaac agagcgaagc
    38041 catcggagat cgctttggcg tctttcgctt tcccgccaca ttagtgttta ccggcggaaa
    38101 ctatcgcggt gtgctgaatg gtattcaccc gtgagcggaa ctgataaacc tgatgcgtgg
    38161 gcttgtcgaa ccacaacagg agcgtgcctc atgagcgaaa cttttttcca tctgctgggg
    38221 ccaggaacgc aaccgaacga tgacagtttc agcatgaatc cactgccgat cagctgtcag
    38281 gtgaatgatg aaccgagtat ggcggcccta gagcaatgtg ctcacagccc gcaggtgatt
    38341 gcgctgttaa acgagttaca acatcaacta agtcaacgcc agccgccgtt gggcgaggtg
    38401 ctggcagtcg atctgttaaa tctcaacgcc gacgatcgtc actttatcaa tacgcttctc
    38461 ggggaagggg aagtgtcagt gcgcattcag caggctgacg acagcgaaag tgaaatacag
    38521 gaggcgatct tctgcggatt atggcgggtg cgcagacgtc gcggcgaaaa gttgctggag
    38581 gacaaactgg aggccggctg cgcgccgctg gcgttgtggc aggcggccac gcaaaatctc
    38641 ttgccgacag attcgctgtt accgccgccc attgatggcc tgatgaatgg cctaccgttg
    38701 gcgcatgagt tactggcgca tgtacgtaac cccgacgcgc agccgcacag cattaatctg
    38761 acgcaattac ccatcagcga ggctgatcgg ctttttctct cacgtctctg tgggccggga
    38821 aatattcaga ttcgtaccat tggctatggc gagagctata tcaacgccac ggggttacgc
    38881 catgtctggc atttacgctg tacggacacc ttaaaaggtc ctttactgga aagttatgaa
    38941 atctgcccaa taccggaagt ggtgctggca gcgccagaag atttggtcga ctctgcgcag
    39001 cggcttagcg aggtatgtca gtggctggcg gaagctgcac cgacgtaaaa agacggtaag
    39061 tatcgctttc agtcttatga atatcgcaat cggcgaatac ctctggtcgt agagtttcag
    39121 gataaagagg gagatctaac attatcgggt tatttttctc tcttcgccta caggagtgcg
    39181 catgtgggat gtcattgatt tatcgcgctg gcagtttgct ctgaccgcgc tgtatcactt
    39241 tttatttgta ccccttaccc tggggctgat ttttttgctg gctatcatgg aaaccattta
    39301 cgtggtcacc ggcaaaacaa tctaccgcga tatgacgcgc ttctggggta agctcttcgg
    39361 tatcaacttt gctcttggcg tggctaccgg cctgaccatg gagtttcagt ttggtactaa
    39421 ctggtcattc tattccaact atgtgggcga tatttttggc gcaccgctgg cgatggaagc
    39481 attaatggcc ttcttcctcg aatccacctt tgtcgggctt ttcttcttcg gctggcagcg
    39541 gctgaataaa taccagcacc tgctggtgac gtggctggtg gcgttcggtt caaatctctc
    39601 tgcattgtgg atattgaatg ccaacggttg gatgcaatac ccgaccggtg cgcattttga
    39661 tatcgacacc ctacgtatgg agatgaccag cttcagcgag ctggttttta acccggtcag
    39721 ccaggtgaaa tttgtgcaca ccgtaatggc gggctacgtg accggggcca tgtttattat
    39781 ggcgatcagc gcctggtatt tactgcgcgg acgggagcgc gatgttgcat tacgctcgtt
    39841 tgccatcggt tccgtcttcg gtactctggc gattatcggc acgctacaac tcggagacag
    39901 ttctgcgtat gaagtcgcgc aagtacaacc ggtaaaactg gcggcgatgg aaggggagtg
    39961 gcaaacggaa cctgctcctg caccgttcca tgtggttgcc tggccggaac aggatcaaga
    40021 gcgtaacgcc tttgccatca aaattcccgc gctgttaggg atcctcgcca ctcactcatt
    40081 agataaaacc gtgccgggtc tgaaaaattt gatggctgaa acctacccac gcttgcaacg
    40141 cggacgtatg gcctggctgt taatgcagga aatatcgcaa ggcaatcgtg agccgcatgt
    40201 gttgcaggca ttccgggaac tggaaggtga cctgggctac ggcatgttgc tctcccgcta
    40261 tgcgccggat atgaatcatg tcacagccgc acagtaccag gcggcgatgc gtggcgcgat
    40321 acctcaggtt gcgccggtat tctggagttt ccgcatcatg gtgggctgtg gttccctgcc
    40381 gctactggtg atgctgattg cgcttgtcca gacgctgcgt ggcaaaatcg accagcatcg
    40441 ctgggtgctg aaaatggcgc tctggagttt gccgttgccg tggattgcga ttgaagccgg
    40501 gtggtttatg accgagtttg gtcgtcagcc gtgggcgata caggacatct taccgacata
    40561 ctccgcgcac tccgctttaa ccacaggaca actggctttc tcactgatca tgatcgtagg
    40621 gctttacacc ctgttcttaa tcgccgaagt ctacctgatg cagaaatatg cccgtctggg
    40681 gccgagcgcg atgcagagtg aacaaccgac gcagcaacag gggtaaaggg gaaaatcatg
    40741 tttgattatg aaacattgcg cttcatctgg tggctgctga ttggcgtgat cctggtggtc
    40801 tttatgatct ccgacggatt tgacatgggg atcggctgtc tgctgccgct ggtggcgcgt
    40861 aatgatgatg aacgccggat agtgataaac agcgttggtg cacactggga aggcaaccag
    40921 gtctggttga tcctcgctgg tggggcatta tttgccgcct ggcccagagt gtatgcagcg
    40981 gcgttttccg gcttttatgt ggcgatgatc ctggtgctgt gctcactgtt cttccgcccg
    41041 ctggcctttg attatcgcgg aaaaatcgcc gatgcacgct ggcgtaaaat gtgggacgcc
    41101 ggtctggtca tcggcagtct ggtgccgccg gtagtcttcg gtatcgcctt cggcaacttg
    41161 ttgctcggcg tgccgtttgc cttcacaccg caattacgcg tggagtatct cggcagcttc
    41221 tggcaactgc tgacgccatt ccctttattg tgcggattgc tcagccttgg gatggtgatt
    41281 ttgcaaggtg gcgtctggtt acaactgaaa actgttggtg tgattcatct gcgttcacag
    41341 ctggccacca aacgcgctgc actgttggtg atgctgtgct ttttgctggc gggttactgg
    41401 ctgtgggtcg gtattgatgg ctttgtactg ctcgcccagg atgctaacgg tccttccaat
    41461 ccgttaatga aactggtggc agtgctacct ggtgcctgga tgaataattt cgtcgaatcg
    41521 cccgttttgt ggatcttccc gctgctgggg ttcttctgcc cattgctgac ggtgatggcg
    41581 atttatcgtg gtcgcccggg ttggggattt ttaatggcgt cattgatgca atttggcgtg
    41641 attttcacgg caggcatcac gctgttcccc tttgtcatgc cgtcaagcgt gagtccaatc
    41701 tccagcctga cgttgtggga cagcacttcc agtcagctga cgctgagcat tatgttggta
    41761 atcgtgctga tatttttgcc cattgtgttg ctctacactc tctggagcta ctacaaaatg
    41821 tggggacgca tgacaacaga aactctccgc cgtaacgaaa acgagttgta ctaaggagca
    41881 ggaacaatgt ggtatttact ttggttcgtc ggcattttgt tgatgtgttc gctctccacc
    41941 cttgtgttgg tatggctgga cccgcgtctg aaaagttaac gaacgtaggc ctgatgcggc
    42001 gcattagcat cgcatcaggc aatcaataat gtcagatatg aaaagcggaa acatatcgat
    42061 gaaagcgatc ttaatcccat ttttatctct tctgattccg ttaaccccgc aatctgcatt
    42121 cgctcagagt gagccggagc tgaagctgga aagtgtggtg attgtcagtc gtcatggtgt
    42181 gcgtgctcca accaaggcca cgcaactgat gcaggatgtc accccagacg catggccaac
    42241 ctggccggta aaactgggtt ggctgacacc gcgcggtggt gagctaatcg cctatctcgg
    42301 acattaccaa cgccagcgtc tggtagccga cggattgctg gcgaaaaagg gctgcccgca
    42361 gtctggtcag gtcgcgatta ttgctgatgt cgacgagcgt acccgtaaaa caggcgaagc
    42421 cttcgccgcc gggctggcac ctgactgtgc aataaccgta catatccagg ctgatacgtc
    42481 cagtcccgat ccgttattta atcctctaaa aactggcgtt tgccaactgg ataacgcgaa
    42541 cgtgactgac gcgatcctca gcagggcagg agggtcaatt gctgacttta ccgggcatcg
    42601 gcaaacggcg tttcgcgaac tggaacgggt gcttaatttt ccgcaatcaa acttgtgcct
    42661 taaacgtgag aaacaggacg aaagctgttc attaacgcag gcattaccat cggaactcaa
    42721 ggtgagcgcc gacaatgtct cattaaccgg tgcggtaagc ctcgcatcaa tgctgacgga
    42781 gatatttctc ctgcaacaag cacagggaat gccggagccg gggtggggaa ggatcaccga
    42841 ttcacaccag tggaacacct tgctaagttt gcataacgcg caattttatt tgctacaacg
    42901 cacgccagag gttgcccgca gccgcgccac cccgttatta gatttgatca agacagcgtt
    42961 gacgccccat ccaccgcaaa aacaggcgta tggtgtgaca ttacccactt cagtgctgtt
    43021 tatcgccgga cacgatacta atctggcaaa tctcggcggc gcactggagc tcaactggac
    43081 gcttcccggt cagccggata acacgccgcc aggtggtgaa ctggtgtttg aacgctggcg
    43141 tcggctaagc gataacagcc agtggattca ggtttcgctg gtcttccaga ctttacagca
    43201 gatgcgtgat aaaacgccgc tgtcattaaa tacgccgccc ggagaggtga aactgaccct
    43261 ggcaggatgt gaagagcgaa atgcgcaggg catgtgttcg ttggcaggtt ttacgcaaat
    43321 cgtgaatgaa gcacgcatac cggcgtgcag tttgtaatgc ataaaaaaga gcattcagtt
    43381 acctgaatgc tctgaggctg atgacaaacg aagaactgtc taatgcgtag accggaaaag
    43441 gcgttcacgc cgcatccggc cactttcagt tttactcttt ctcggagtaa ctataaccgt
    43501 aatagttata gccgtaactg taagcggtgc tggcgcgttt aatcacacca ttgaggatag
    43561 cgcctttaat attgacgcct gcctgttcca gacgctgcat tgacaaactc acctctttgg
    43621 cggtgttcaa gccaaaacgc gcaaccagca ggctggtgcc aacagaacgc cccacgaccg
    43681 cggcatcact caccgccagc atcggcggcg tatcgacaat caccagatcg taatggtcgt
    43741 tcgcccattc cagtaattga cgcatccgat cgcgcatcag cagttcagac gggttaggtg
    43801 gcacctgacc gcgagtaatc acatcaaagc ctcctttgcc aaaatgctgg atcactttgt
    43861 tgagctcatc tttacctgcc agatattccg acaagccatg ttcattactc acggtaaaca
    43921 ggttatgcga ataaccacgg cgtaagtcgg catcaataaa taacactttt tgatcggact
    43981 gggcgatcac cgctgccaga gttgaactga caaacgtttt accactgtct ggcgtcgcac
    44041 cggtgatcat cagaatgtta ttctccgtct ccatcatagc gaaatgcaga ctggttcgta
    44101 gcgcacgtac ggcttccaca gcagaatccg ccgggttatc caccgccagg aaggggatat
    44161 ttttagtacg atggcgctgc tgattagaaa ataaattttt cttacgcaga cgggtgcgtt
    44221 tatccagcca ctcggacatt gggatagtgg cataaacgct gatgccgtgc tcttccagtt
    44281 gttccggggc ttctacacca cgacgcaaca tcgcacgcgc cagcacggca cccacagaaa
    44341 taaacaggcc aagaataaaa ccaagcacca cattcaacgc ttttttcggt ttcactggct
    44401 gcggctgagt gactgccggg tcgataatcc gcacgttacc aatggcactg gatttcgaaa
    44461 tactcaactc ctgctggcgg ttaagtaatt gcagatatac cgcacggccc gcttctacgt
    44521 cacgacttaa acgcaacact tcctgttggg tggaaggcat tgccgatacc cgcttattca
    44581 ggcgtttgcg ttcttgctcc agcgtctggc gtttttccag cagcgcacga taagttgggt
    44641 gatctttctt atacagctgg gagatctctg cctcgcggaa ggtcagctca ttgagttgat
    44701 tatcaacgtt cacaatctgc tcaagaacgg ctttggcttc caggttaagg tcaaccgaat
    44761 cgcgctgctg gcgataaacg ttgagttttt cttccgcctg gtccagctcg ctgcgcactt
    44821 caggtaactg gcgctgtaag aattcaaggc tttgtgaatc ctgcgccgcc tggcgagcga
    44881 tattctgttg caaatagttg ttagcgatgc tgttcagaat acgagtaatc aactggggat
    44941 catcaccagt catggtaagt tccagcatcc cgctttcttt actgcgttcg ctaacggtaa
    45001 aggtttcctg caatgcgtta atcgcttcca gttcggtacg ctggctcagg acaaactgtg
    45061 ttcctggttt ggccttaatg tccgcgatag tcagcgcaac gccatctttt tccagacgct
    45121 ggccgaccat accattgacg gtgaactctt caccttccag tgtatagtgg ccgttttccc
    45181 caaccgtgag tgtcagttgc tgatcctgac cattcagttg tggaatatgc atccagctga
    45241 tcgccagctc acctggtttt tctttggtta atctcgccca gccgcgaccc acaatcggaa
    45301 aatacttctg ctcaactatg tcgcgcagat tcagttcagc aatggtttta ccgagaatca
    45361 tgcgcgattg cagcagttgg atctccggtg cagactcggg cgatgagtta gggatcatat
    45421 cgctcaggcc gctgagaatg gcgttgccct gtttttgctc aacctggacc agagtatctg
    45481 cctgataaat tggtgtgctt aacagcgagt aagcgacagc gatcagcgtg aataacgcgg
    45541 tcacgctgat aataaactta cggtgatccc ataactcgcc gaccagacga agcagatcga
    45601 tctcattttc ctgagtgctg cctggtggcg tattcatatt tttagttgtc atcgtatttt
    45661 ataaactcaa attatcggct gaggcgcttc gcccattcct gactggcgcg ctccaacata
    45721 ccgtagacat gttcaaatgc gtcctgactt ttacgatagg gatccgggat ctctttctgt
    45781 tccagccatt gcccaaacag cattgtcttc ccgcggacct cgggagcaat tgcggtaacc
    45841 tgagcaatat gttccgactc cattgccaga atcagatcgt aatttcgtgc catctcagca
    45901 gtgagcttac gtccggcatg tccttcaaga gatacgccat gattagcggc aacatctgcc
    45961 gctgtcgcat ctgcagggtg ttttatcaga ccatgaacac ctgccgattt gacttttacg
    46021 cccggcagac gtttacgcag caagcgctcg ccaatgggcg aacggcaaat gttgccggta
    46081 caaaccacca ggattgagtt aaattttagt tgggccatgt ttgaatccac cgtgaagtct
    46141 ctgtcaggtc atgcaccccg gaaatcgtcg gtaccagcag agaaatcaca cggttccaac
    46201 gagccagcgg cgcggtagtg acataaacaa tgtcgtaagg ttcaagctgg aattccgttc
    46261 cgagtatcat tgccgatgcg tccttcgcat tcagctgata aatattggcg attttgccat
    46321 tctgcttgtt ttgcgttgca cgaataacaa aaatccccgt cgcatcagcg acatcctgat
    46381 tcataccctc agcgttaccc agcgcttccg ccagggtcat accgctgcgg tccattttca
    46441 gcgtgctctg tttacccact tcacccatca cgaagacttt cagatcatcg ttgcgcggaa
    46501 taaataaaat atcgcccggg tgcagcagtt tgttctggcg caagtcgcca cgctgcatca
    46561 gcgcatagag gttcactttg gtctttacgc cattctgggt gagcaccacg ttgcgccagt
    46621 ctgcatctgc ggtcagaccg cccgcggcgt taatcgcatc catgatggtc agaggaatat
    46681 tggtaatcgg ctgctgaccc gatttgctga cttcgccggt gacgtacgct ttttgcgatc
    46741 ggaacgccgc gacgctgaca tcgacttgcg ggctttcaat gactgaatcg agacgcgctg
    46801 taatctcgtt gcgtacctgg gtaagtgttt tcccggccac tttcaggcga ccaatatagg
    46861 ggtagaaaat cgcaccatcg gcgttaaccc agttgccggt atcactggcg ctacggtatt
    46921 gccctgcggg ggtggttagt tccgggtgat cccagaccgt aaccatcaac acatcaccaa
    46981 tcccgatacg atattcatag tttgcgattt gctgctccag ctccgggtta gcctgcgaca
    47041 taatcggttt ggcgcgcaac tgatcaatca atcctggcgt gacgggatag atattgacca
    47101 ttttgtccag gtcatactga ttgtcaggta gttcaatgac atccttgtta ctggtcgata
    47161 aattctgacc aggaacaagg gtacatgctg tcaaagacag aacagccaat gtcagtaccg
    47221 aaaacttaaa aatgttcttt ttcattggtt tatatttatc agtcaatgta atgttatgaa
    47281 ttacgtcagg ttattgtcca accggtactt ctcgctctgc cgtcattgga tagagctggt
    47341 atttgcgtga cagcatttgt ccaccatcac gcgtcaatgg tgtccacgaa accaccgcgc
    47401 ggttgcggtt aggtccaatg gtcatcaagt cgaacggaat cgagatataa aaacctttac
    47461 taaagccgcc ttcgccgtag tcatctttag atacgttact gattgccgcc cataccccta
    47521 ccgccacgcc gctgtcaaag cgtttggcga cgtcgatcgt tgccccttta tcttttgcca
    47581 gatattgccc aacgctaagt ttcatcagta cgccattgag cgtcggcggg ttccagtaag
    47641 ccgtcacgaa accagttggc gtggaataat cggtgaagcg catcatgttg tcccagtcac
    47701 gttgcttcac gtagttaacg tccacaccca gcgcccagct ggcatctagc gggcgataaa
    47761 gcagctcgga accgacaccg gcgtacatcg tttccagata accgccatac acctggccat
    47821 agaaaccatt gcctaagtcg gcaaagtagt tcgcctgcaa gttgttgaga taaatgtcat
    47881 tgcgaacgta atcacggata tgcgtgcgca cgcggggcag ggtagagtcc gcgggcaaca
    47941 gcgaagactt aaacttgtcg tagttgttgt aaatattggt gaaaataccg ccatcaagca
    48001 gcaggtggtc ggtaaaccag tagcgggcac tggacatcag ccccagctgg aacatataga
    48061 aatcttccgg gccgcccagc gactgtgaaa gtgttgggtt gaaagagtag ctaaagcgat
    48121 cttcacgaat acggtagccc cgaccaaagg cagaaagatc ttctgcttca acacgttgtt
    48181 gttgaagttg ctctgattga ccaggcgctg taccagccag ctgtttgcgc aggctggcta
    48241 cgtcggtttc ggtagtcacc atcgccatat gctcgcgctt ttgcgtcacg ctaatcttct
    48301 caacgccttg cggcaggtta ttcaccagaa tccggttggc acgatcgacc gcttcgcgag
    48361 aatcacggta tttgtattgt tgaccagaca tatacagcgt cttatcgcgc agctgaattt
    48421 ccggtgcttc aaaacctgcg ttgtacttca gcgcggtaag ttggttcgct actgtggtgt
    48481 actgcaatcc ttcagattca ggcgcaggtt gatatgccgg ttttggcgta tcgcgcaggg
    48541 cagggcgcag atcgttgaaa ttggtccgta acgtgaagcc aaacatcaac gtgttaccgc
    48601 gttcataact caggttgaga tctgcccagc tggcagcgcg ataaactgcg ccgacgttga
    48661 aatggcttgc ctgaggcagt ttgccagcga aatcattctg gtaattgttg ccatcgtatt
    48721 cgagtttcag acgcagggga ttccacggcg tttgatactc aatgccgcca aagatggaag
    48781 ccgggccacg aaagatatcg ctaaagctga tatcgcccgc atcgtgagac tctgcgcgat
    48841 gacaatattt atcgctcacc cggcaaaacg ggttggtaat attgcccgca ttaccggcgt
    48901 agccccatgc catcccgagg gtgaaatcaa atggccccgc ttgcttgctg gccaccagat
    48961 actcaccgtc aaacagaccc gtaccagcaa tatcacgttt accaaacgcc acttgcggta
    49021 gccagtaacc ttcttcccac aggcgaagtt taaaatcgaa tgatttgtct ttgtatgact
    49081 gatcgccgct gaaatcttcc cactggctat atttgcgtgt gcgcacatcc gtataacgaa
    49141 tggtgccttc cagccatggg aacagcgcca cggaggtgga gtagaaccgg tattgatcgt
    49201 tatcccggta gttgacgctg aattcacctt ccggtgcgat gcgcgcattt ggcatctgca
    49261 acaatcctgt gccgccaaag tctgattgcg acgaacccag cggatccggg taggttaata
    49321 cttcagcatg acaggcactg gagacggcaa tggccaggca gcttaaaaga taagaattct
    49381 tcttcatcag tcaggaacac gttgcgttaa aacagacaca atctggtcat ttaaatcagc
    49441 atatttttcg ggcagtacat gcgcagaaaa gcccaaccaa agctggctgc caggcggtgg
    49501 ttccacatgt cttttattcc acagggcaac aggggcgacg acagtctcac cctcaggcgt
    49561 aatcaccata acgttattct tgtccgcgcc tgcaaggcgg gggtgatctt gcagataatc
    49621 ggtaacgcta cggcctgcct gccagggcaa ttgcccggcc ccagaaaccg cacccagcag
    49681 agtaatagtc accggacgct gtacggtata gagcgtatag tccccgacca acgggggatt
    49741 gctgttctca tccactcgta caaaatcagg gtccagtttg accggtaagc gaccagtgat
    49801 attgagattg agtaactgct ggcgcacgga tttaatcgtt gccgccacat cgtcgtcagc
    49861 ttctgcttcc caggaagcaa gctgtgccat gacatgttgg tagtctttaa gcgctttagc
    49921 tttcgccgca gagtcggtca gcaaggcacc aggccaccag aggcgatcgc gcagttgcgg
    49981 ttgtgtcacc aactggacaa cattttccat ggggccaacg gaaagtgtct gttgttcgcc
    50041 aggcagataa atagtcaccg ttccttgcgc aaaggcatgg ggtgtcatta cgtaaagtac
    50101 gctggcaatg aaatacgact gtaatttatt catggtgcgg gtttaagaaa cgtcatttct
    50161 acgggaataa cccctgcgcc taacatttgc cgactttgac gaacctggcc agtagccgaa
    50221 tcgacccaga atgtgttttg ccactgtttt tctggccgcg tggaagtcac ctcttcctgc
    50281 cagacattac acagcacatt gcgacccgca atattcagcg tctctaatcc agcaaaggaa
    50341 aaagtagaac tcagggtcgc agagcgaaaa tggttgtctt cactccagag aatatcgcgc
    50401 gtccagcggc tgccatcttt tattgccagc gcgttacgca gagggtcctg cccggaattt
    50461 gtcacttcca gcagattatt attaagtttg acggttttta aaagacgtcc attgtgggta
    50521 accagcatgg cgttatcctg ggataaccat ttgctttgtt cttgttctat atatcccagt
    50581 accacaaaga ttcgttgccc ttcattaagg cgtaaataca tcgtggaata aggcaacgcc
    50641 tggatctgct gatccgctac ggtgatatcc tgattatcga aaaggctggc gcgaaatgta
    50701 tcgaccatac tttgctggct gtgcgtacat cccgcaagaa atagtgcgaa aatcgataaa
    50761 ataagagggc gcacctttaa tccttgaaag gttaattaaa aaaatgcaaa tctctaaagc
    50821 ccggtacgaa tatcgcaacc gggtatcatt taactttaat ttcgacgcgt taaagcagta
    50881 ttagcgtgta gtggacgtgg tggttgaggt gttatgagaa ccgccatcgc cactgtcatt
    50941 tgctgcaaca actgccaacg tcgccacacc aacagccgtt gccacaccga caactgtcgc
    51001 cgtgccgaca gcgccagcag ccacgccacc agtggtagta gcagtcgtgg ttgcgccggt
    51061 agtggtcgtt gtggtgccag tagtacccgt cgttgcctcg gtcccgtttg ttgcttcaga
    51121 ggcagcaaac gcagctggcg tcgtcaacat gaaggccata aggattgcag aaagcttgtg
    51181 tttcataact tttcctttat tcatcgcatg gacaatacgt acgcttgaga tcaggattgt
    51241 ctactttgag tataacatcc ttgtgaaatc tccctttcct cataccgctt gcactatata
    51301 ctgatactgc gggtaggaaa ggtgcgcaga gggaaatata agattattaa cgatcccctc
    51361 tggaaatacg acttgtgagc ataagataaa tcttagcaaa atacaaatta aagataagga
    51421 aattgttacg aaagctatta attattgttg gtaatatagt ttcaagtggt actataactg
    51481 gctgttaaat ggcatttata actattaggt gcagagatat tcgcttaaat ggattagttt
    51541 ctttgtggaa aagtaactga tgttataatg tatgatgttg actattgcac tattccaata
    51601 aaaccagtca gctttaaaca agcagcgtca tattaagaga gataaacatt tgccgctgtt
    51661 ggtcctcgca ggccatttac gcggcaaaat tccacacgta atcctggtat aagcacttct
    51721 gcgtcgcggg gagtgaatgc ggaaatatgg acctgaactt ctttacgacc gtcggagggg
    51781 ataatgaatc ctttgccgct tttgcgatca aaggttttga caattcctgt cattttacgg
    51841 gacaaacaaa ttccttactg aaaatactgc gctgcactat acggggttaa taaaataaag
    51901 ccagcgatat ttaagaccgc cggacggcta aaataaaatt tgcttaatct caattatcat
    51961 gcgttaatag ctgcgtcggt ttgaaagaca gacagcatac aaagtagttt actaaagcag
    52021 ttctcattat caggcattat ccccttcttt tgagtctctc tcctgaacac taagtagttt
    52081 ctgtattaaa gccctgtttg ccgaaaggcc caaaatgaag gaagtaaaat atgtctaata
    52141 aaatgactgg tttagtaaaa tggtttaacg cagataaagg ttttggcttt atcactcctg
    52201 atgatggcag caaagacgtt ttcgtccatt tcaccgccat ccagagcaat gaattccgca
    52261 cgctgaacga aaatcagaaa gttgaatttt ctattgagca ggggcaacgt ggccccgcgg
    52321 cagcgaacgt tgttacgctc taaggttgcc gttattactc aacatctcca tttccgctgt
    52381 ccatgttgtc atggttcaca gtaccgcaca tcggcattcg atgtgacgga gcgaaaccct
    52441 ttgggcgcta agtgtatttt ttgtaaatcg acgatgatca cctttgataa cgtcgcgctg
    52501 caaatacgca ctgaccatgc gccgctggat ttcacaaaat aatatcaggc tccctcgtgg
    52561 agcctttttt atatctgcct tatttttctt caacgctgta tgtatagtaa gcgataacct
    52621 gttgattatt gaatctttcg gggagatggc ttataacatt tcttacctga ccagggtacc
    52681 gggaaccaac accttactgg cgtgttgctg tcttttaaga ccagaagagg ttaacagtga
    52741 atattgaaga gttaaaaaaa caagccgaaa cggaaatcgc cgactttatc gcgcaaaaaa
    52801 tcgccgagct gaacaagaat acagggaaag aagtctctga aattcgcttc accgcacgag
    52861 aaaaaatgac cgggcttgaa agttatgatg tcaaaatcaa aataatgtga ttttgtgaac
    52921 atcaccccgt gcgaggtgat gttccgcttg ttgctaattt agtgaccaat cattggcgct
    52981 tgtggaatta agcgtcggta caattcctcc ggcaccgggc tttgccatac tcccgcatac
    53041 attgcgtaac caatcaccgc gaacataatc ctcagaacca gtagcgtcat taaccagcca
    53101 gacaacgcaa aagctttttt atttgccgca ggtttttgca gtgaaaaggt caatgttgag
    53161 gctaccggac atgactctac gcaagtcata cagccggtac attccactgt tcgtacctga
    53221 attaatttat ctaccgggat ccgtgatggg caattttttg cgcatttgcc acagccgata
    53281 caactttcgg cattgcgacg aatcttaaac ggcgataata gcgaaaccac gcccatcagc
    53341 gcgccatatg ggcaaagata acgacactag gcatggcgaa taaacaggct ggcaatcagc
    53401 aaaacggtca cgctgattaa tgtcgcggtc cccatatgac gaaagaaatc gagcattttt
    53461 acgtccatca ccacgctgta gggcgacaac ataaaatagt gaatcgcctg agcgggcatc
    53521 aataacgcga tatagagaaa aaaactcaac agcaaatact tcacgccgcg cagaggaata
    53581 tccagccagc ggggaaggac acattgccga ccaaacagtt tgttaccgag atcgccgatt
    53641 aattcagaaa gcgtaccaac cgggcataac catgagcaaa aggccttttt gagtaataga
    53701 ctgatgacga taaaagcgac caataacagc atcgcggcgg cgtggacgga cggtaactga
    53761 cctgttgtaa ggctatattt cagattcatc agaccggcaa tcggtagcca gccttcgata
    53821 cctcccggtc tggcgacaaa tgtcgtgcta cttgccgttt cgtaatagcg cacccaatac
    53881 cagaacgtga tggcaatata aatattcatt gccaacagta ataattgcgt cgctttacgc
    53941 caggtcgtgg cattgcgcca gtcattccac ggtaatttgc cgcccgtcgt gcctggccgc
    54001 cgctgccagc gggttctttt tttctctgcc atgattttgc cagtccgtta agttgcatac
    54061 caaatgccac tattctagtt gttcttaact ggctgatatt gattcaaatc gcgttcaggt
    54121 ctttcttatg caaccatgct tccagagcgg caacactgcg tgtaatttct tcgtgtggaa
    54181 ggggggcaga taatggctgc tgctccagtt gtgcgcatag ctggctggcg atacgcattc
    54241 ccaaactcga gcaactgctt tttagctgat gcgcggcacg ctttattttc tcgctatcct
    54301 gactggcgcg ggcaatgtcg atttcatcga gaagcggcag ggcatgttgt gtaaataata
    54361 ccagccattc gtggatcttc tccgtcccca ttaactgagc atcttcattg agttgcgata
    54421 catccagcga ttgatcgtta ttgacttgca gttggagata gtgcgccagt aactgaccga
    54481 gcacttcacg cggcaccggt ttagggataa tcccgcggaa taatgaactg gtacgctggc
    54541 gcagcgtttc gtcaatgaca tgggcgctaa agccaatcaa aaccagcgac ggatattgct
    54601 gtgccagttg tcgggcaagc gtaatgccgt cgacatccgg cagatcaaaa tccaccagtg
    54661 cggcagcaaa cggttcgcta ttttgcagtg tctctaaagc ctgcgcggca ttgccaacag
    54721 caacaacctg cgcaccactg gtgttcagca tctcaacggt aattcgctgg gttagcgggt
    54781 tatcttcaat taacagcaaa cgtaaaccgt caagacgcac cgcctgattg actgtttttg
    54841 gcacgggtgc cgtggcaaca cgtaacggca agcgtaaaca aaaacagctt ccaacctccg
    54901 gcgtgctggt ggcgctcagt tcgccgccca tcgcctgggc cagacggcta ctgatagtca
    54961 gccccagccc ggtgccgccg cgtttgccgc ttacctgcac aaatggctgg aagatttctg
    55021 ccagtttcgc gggatcaata ccgcagccgc tgtcttccac ttcgaccagc cattgctcgc
    55081 catcagtgcg actacgcagg ataatgtacc cttcgtcagt aaaacgcagg gcgttgctca
    55141 acaggttggt tataacctga cgaatacgtc gtggatcgcc cattaacgcg cacggcatat
    55201 catcggcaat tgccgttgcc aggcgaatcg ggcgaccttt cacccgtccg ctcattaatt
    55261 gcagggtact ttccagcagc gggcgcggtt caaagggctc atcgctgacc gaaacattct
    55321 tgccacctgc ttcgatagcg gaataatcga gaatatcgtt gaggatggtc agcaacgatt
    55381 caccagagtc agtaattgcc cgcaaatcat cacgctgggc gttaagtgcg gggttatctg
    55441 ccagtagctg tgcggtgccg agaataccgt acagcggtgt gcggatctca tggctcatcg
    55501 ccgccagaaa cgccgatttt gcctggctgg ctttttctgc ttccgcccgt gcctgtcggt
    55561 gttctatcac tagttcctgc aattcagctg tacgcgcttt gacctgcgcc gccagctgtt
    55621 cacggtggcg attcagtgca tgaacactgc tgcgaaacgc atccatcagc tgcccgatgg
    55681 tgtccagctc ccgtacgcca gcggtttccg ggaaagggga gtcaatatca ccgtccagca
    55741 gccgttgcag cgcctgcgtt tgttcggcaa gtggacgcgt gactgagcga taaaccacgc
    55801 gccagaggat cagaatcagt gcgcaaagtg aaaccatccc cagcaataac aggctgtatt
    55861 gcccgcgtgc actggctttt tcctgatgcg ccagtccgtg ctgattacgc agctcaatgg
    55921 tgtcgaccag ctgactgact tcgctactaa actgcgcgaa ctgggcgata ttattttggg
    55981 cgagagtttg caggtgattg ctgatttcac tgtcctgctg gaacagcgcc aacagatcgc
    56041 tatattggct aacggtagtt aacgttgttg cgacctgcgc acgaactccc ggatcttcaa
    56101 tgcgtatttg ccgacgttgc agaattttca ccgcattatt gagctgcttt tccagcgttg
    56161 gtgcattttt ctggatctgc tccagcccca gattcatcac catttgctgc acccgcagag
    56221 cgctaaggcg cagttcattc atctggttaa catactcaag atcgatatca atcagccgat
    56281 cgagtgcact ttcagcagcc tgacgctgat cttgttcgat caaatcgtaa atcccggcct
    56341 gggtcgctcc agcggaagtt gtcgcattat tcgcctgacc ttgcgccagg cgtgcgatct
    56401 catcggcggc agcgactatc tgctgactga gttgctgttg ttgctggcgt agttgcagac
    56461 gccgccccac cagttcccct tgctgacgta acgaacggga gatctcctgc tcctgttgtt
    56521 caatagcggt ggtatcaaaa ccttgttccc gtaacgcttg cagcaacgca ttaatcttca
    56581 ggctttgtgc ggtgagcatt cgcccctgcg cctgccacat cttttcgtta tcggcactgg
    56641 tcaggttctg cgcggcgaaa agttcccagg cgctggcttc gctcaactgg cgcgccatat
    56701 tcatggtagg aatcaatgcc tgagtattgt ctttttccac ctggctgata aagcgcaggt
    56761 tgtaccatcc caccagggta ctggtcaggg ttaacagcgc catcagggca aagcccatcc
    56821 agagtcttcg ggtcagggtt aaattcacgg tcggtgcact ttaggtgaaa aatgttgagt
    56881 cgcaaagcgg aatgcatcta gcataaagcc ttattattga tgaggctatc atgcgcgtac
    56941 tgctattttt acttctttcc cttttcatgt tgccggcatt ttcggctgat aacctgttgc
    57001 gctggcatga tgcgcagcat ttcacggtgc aagcctctat gccgcttaaa gccaaacgcg
    57061 catggaaact gtgcgcgctt tatcccagcc tgaaagattc atactggtta tcgttgaact
    57121 atggtatgca ggaggcagct cgccgttatg gtgtggattt aaaagtgctg gaggcaggcg
    57181 gctacagcca gttggctacc cagcaagcac aaatcgacca gtgtaaacag tggggcgcag
    57241 aggccatttt actcggtagt agcacgacct catttcccga tctgcaaaag caggtagcaa
    57301 gtctgccggt gatcgaactg gtaaatgcta ttgatgctcc ccaggtgaaa agccgcgttg
    57361 gtgtaccctg gtttcagatg ggctatcaac ctgggcgata tttggtgcaa tgggcgcacg
    57421 gtaaaccact gaacgtgctg ttgatgcccg gacccgataa tgccgggggc agtaaagaga
    57481 tggtcgaggg ttttcgcgca gccattgccg gaagcccggt gcgtattgtt gatattgcgc
    57541 ttggtgataa cgatattgaa atccagcgta acctgttgca ggagatgctg gaacgccacc
    57601 cagaaatcga cgtcgttgcc ggaacggcca ttgcggcaga ggcggcaatg ggtgaagggc
    57661 gtaacctgaa aacgccgctt accgtggtgt cgttttatct ttcacatcag gtgtatcgcg
    57721 ggctgaagcg gggaagagtg attatggctg ccagcgatca aatggtctgg cagggggaac
    57781 tggcggttga gcaggccatc aggcaattac aggggcaatc ggtgtctgat aatgtcagcc
    57841 caccgatttt agttctgacg ccgaaaaatg ccgaccgtga acatattcgc cgctcgctgt
    57901 caccaggggg atttcgtccg gtctattttt atcagcacac atcagcggct aagaaataac
    57961 cttcaccatg ttgcgtcacc agtaaatccg cgctgagttt atgacgtaaa cgacgaatta
    58021 acacatcgac ggtgcgcagg tcagggtttt ccacccgacg cgcagaaagc atacgtagca
    58081 gacgttcacg gctgagaatt tcgcccggat tcgtcacaaa tgccaccaac atttcatact
    58141 ctgcgcgggt cagtttaatc ggctcgccat cccgctccag cgtatggcgc gacacattca
    58201 ggcaataacc ggcaaagcga tagcagttgt cctgagtgtg cggttgagct tgtcgcgcga
    58261 ggtcgattcg ccagagcaga tttttcaccc gtactaccag ttcgcgcagt tccagcggtt
    58321 tggtgacgta atcgtctgcg cccatttcca gcccaacaat acggtcaatc cgatcgctgc
    58381 gtccggtaac cagaataatc cccaccgttg agcgttctcg cagggcgcgg gttaacatca
    58441 ggccattttc atcgggtaag ttgatatcca gcagaattaa atctaccggc tgattctgca
    58501 taatttcccg cagcccggca ccgctcgctg taacggaaac ggtatacccc tcctgagtga
    58561 agtaagattg taatcgcgcc tgggtaaccg gctcatcttc aacaataaca atgtgatgtg
    58621 gcatcagagg gttttactca ttctgttcat attctgccgt aagccgttca tcctgaccag
    58681 tgccgctgtt catatttgct cattaagatc gcttcactaa accataattt tacaggggct
    58741 attatgcgga aactctggaa cgcgctacgc cgacccagtg ctcgttggtc ggtactggcg
    58801 ctggtcgcta ttgggattgt gattggcatt gcgctgattg tattgccaca cgttgggatc
    58861 aaagtcacca gcacaaccga attttgtgtc agttgccaca gtatgcaacc ggtgtatgaa
    58921 gaatataaac agtcggtgca ttttcagaac gcctctggcg tgcgagctga atgccatgac
    58981 tgtcatatcc cgccggatat tccaggcatg gtgaagcgca aactggaagc gagcaacgac
    59041 atttaccaga cctttattgc ccactccatt gatacacctg aaaaattcga agccaaacgc
    59101 gcggaacttg ccgagcgtga atgggcgcga atgaaagaaa acaactcggc aacctgtcgc
    59161 tcctgccata actacgatgc gatggatcat gccaggcagc atcctgaagc ggcacgtcag
    59221 atgaaggtgg cagcgaaaga taatcaatcc tgcatcgact gtcataaagg tattgcccac
    59281 cagttaccgg atatgagtag cggcttccgt aagcagttcg atgagctgcg cgccagtgct
    59341 aatgacagtg gtgacacgct gtactctatt gatattaagc caatttatgc ggcgaaaggc
    59401 gataaagaag cctctggttc tctgctgcct gcttcggaag tgaaagtcct taaacgtgac
    59461 ggcgactggc tgcaaattga aattaccggc tggacggaaa gcgccggacg tcagcgtgta
    59521 ctcacccaat tcccaggtaa acgcatcttt gttgcctcga ttcgtggtga tgtgcagcag
    59581 caggtgaaaa cgctggagaa aaccaccgtt gccgacacca ataccgagtg gagcaagttg
    59641 caggccaccg cgtggatgaa gaaaggcgac atggtgaacg atatcaaacc gatctgggcc
    59701 tatgcggatt cgttgtacaa cggcacctgt aaccagtgcc acggcgcacc ggaaatcgcc
    59761 cactttgacg ctaacggttg gatcggcacg ctcaacggca tgattggctt taccagcctc
    59821 gataaacgtg aagaacgcac cttgttgaaa tatctgcaaa tgaatgcgtc tgacaccgca
    59881 ggtaaggctc acggcgataa gaaggacgaa aaataatgaa caataacgat ctctttcagg
    59941 catcacgtcg gcgttttctg gcacaactcg gcggcttaac cgtcgccggg atgctggggc
    60001 cgtcattgtt aacgccgcgc cgtgcgactg cggcgcaagc ggcgactgag gctgtcatct
    60061 cgaaagaggg cattcttacc gggtcgcact ggggggctat ccgcgcgacg gtgaaggatg
    60121 gtcgctttgt ggcggcaaaa ccgttcgaac tggataaata tccgtcgaaa atgattgccg
    60181 gattgccgga ttgccggatc acgtacacaa cgcggcgcgt attcgttatc cgatggtacg
    60241 cgtggactgg ctgcgtaagc gccatctcag cgatacctcc cagcgcggtg ataaccgttt
    60301 tgtgcgcgtg agctgggatg aagccctcga catgttctat gaagaactgg aacgcgtaca
    60361 gaaaactcac gggccgagtg ccttgctgac cgccagtggt tggcaatcga cggggatgtt
    60421 ccataacgct tcggggatgc tggcgaaagc tattgccttg catggtaata gcgttggtac
    60481 gggcggagat tactctaccg gtgctgcgca ggtgatcctg ccgcgcgtgg ttggctcaat
    60541 ggaagtgtat gaacagcaaa cctcctggcc gctggtattg cagaacagca aaaccattgt
    60601 gctgtggggc tctgatttgc tgaaaaacca gcaagcgaac tggtggtgcc cggatcacga
    60661 tgtttatgaa tattacgcgc agctaaaagc gaaagtcgcc gccggtgaaa ttgaggtcat
    60721 cagcatcgat ccggttgtca catccaccca tgagtatctg gggcgcgagc atgtgaagca
    60781 cattgcggtt aacccgcaaa ctgatgtgcc gctgcaactg gcgctggcgt atacgctgta
    60841 cagtgaaaac ctgtacgaca aaaacttcct cgctaactac tgtgtgggtt ttgagcagtt
    60901 cctgccgtac ctgctgggtg agaaagacgg tcagccgaaa gatgccgcat gggctgaaaa
    60961 actgaccggc attgatgccg aaaccattcg tgggctggcg cggcagatgg cggcgaacag
    61021 aacgcaaatt attgctggct ggtgcgtgca gcgtatgcag cacggtgaac agtgggcgtg
    61081 gatgattgtg gttctggcgg cgatgctggg gcaaattggc ctgccaggtg gtggctttgg
    61141 ttttggctgg cactataacg gcgcaggcac gccggggcgt aaaggcgtta ttctgagtgg
    61201 tttctccggc tctacgtcga ttccgcctgt tcacgacaac agtgattaca aaggctacag
    61261 cagcaccatt ccgattgccc gttttatcga tgcgatcctc gaaccgggga aagtgatcaa
    61321 ctggaacggt aaatcggtaa aactgccgcc gctgaaaatg tgtatttttg ctggaactaa
    61381 cccattccat cgccatcagc agatcaaccg cattattgaa ggctggcgca agctggaaac
    61441 ggttatcgcc atagataacc agtggacctc aacctgccgc tttgccgata tcgtgctgcc
    61501 tgcgaccacg cagtttgagc gtaacgatct cgaccagtac ggcaaccact ccaaccgtgg
    61561 cattatcgcc atgaaacagg tggtgccgcc gcagttcgag gcgcgcaacg actttgatat
    61621 tttccgcgag ctgtgccgtc gctttaatcg cgaagaagcc tttaccgaag ggctggacga
    61681 aatgggctgg ctgaaacgca tctggcagga aggtgtacaa caaggcaaag gacgcggcgt
    61741 tcatctgcca gcgtttgatg acttctggaa taacaaagag tacgtcgagt ttgaccatcc
    61801 gcagatgttt gttcgccacc aggcattccg cgaagatccg gatctcgaac cgctgggcac
    61861 gccgagtggc ctgattgaga tctactcgaa aaccatcgcc gatatgaact acgacgattg
    61921 tcaggggcat ccgatgtggt ttgagaaaat cgaacgctcc cacggtgggc ccggctcgca
    61981 gaagtatccg ttgcatctgc aatctgtgca tccggatttc cgacttcact cgcagttatg
    62041 tgagtcggaa actctgcgtc agcaatatac ggtagcgggt aaagagccag tattcattaa
    62101 cccgcaggat gccagcgcgc gcggtattcg taacggtgat gtggtacgcg tctttaacgc
    62161 tcgcggtcag gtgttggcag gggcagtggt ttctgaccgc tatgcacccg gcgtggcacg
    62221 aattcacgaa ggggcatggt acgatccaga taaaggcggc gagcctggtg cgctgtgcaa
    62281 atacggtaac cccaacgtgt tgaccatcga catcggtaca tcgcagctgg cgcagttgtt
    62341 ctcccgtgaa ctggacgatg aacaactgac gcaaatcgcc agtgcgcaga tggctgaatg
    62401 gttttcgttg ctgaaaagcg aaccgccgct cactgcggcg gtgaatgcgc tggaaaaccg
    62461 tattgccgct ctgacagtac gtgacgatgc ccgtctggaa ctggccgctg acttttgcgg
    62521 cctgtttctg atgaccgaca aacaagcggc gctgccgtat gcatcggcct acaaacagga
    62581 cgagcaagag attaaacgct tgttagttga ggcagggatg gaaaccagtg gcaatttcaa
    62641 cgaatcggca gatcatctgg cgatctatct cgagttgttg agtcatctgc atttttcgct
    62701 gggagagggg accgttcctg cgcgaagaat cgacagtttg cggcaaaaaa cactgacggc
    62761 gctgcggcaa tggttaccag agtttgctgc gcgttgtcga cagtatgaca gctttggttt
    62821 ttacgcggca ctaagccagt tattgctggt gttagtggag tgcgaccacc aaaacagata
    62881 acgtcgttta tgcgcctgaa aagacgcgtt tagcgtcgca tcaggcatta tggcgcagtt
    62941 gccggatgcg tcatcacatc cggcaaacac acatcacgga tgagctacaa accgggaaag
    63001 ccgctggcgt agcaggcggt tttcctgctt caggtgcgca atatcatcca ttaacgtcag
    63061 cgccaccgcg atccccggcc agtccagagc cagttcatga cgcaggcgta ccgcgcgttg
    63121 caccacaatg gcggcatggt cgtcaaatac ccaggttgtt tcctggatct cacgcggttc
    63181 aaccaccccc aaaccgacaa tttcattcaa ctcctcttca gagatgccgg tatgcaggca
    63241 aaattcggta atagtaaaag tcaccgtaac attagccatc atgctttccc ccaatcttta
    63301 cgtggatcaa aagacgactg ggcgtctgcc agttgctgcc acagcgcggc agtgttttca
    63361 tccggtttcg gcggcatcac gattttcagt accgcataca gatcgccggt ctgttttttg
    63421 ctcaccagac ctttgccttt aacgcgcaat cgttgcccgg cctggctgcc tggcgggata
    63481 gtcagcaaaa tgctttcttt cagtgttgga acggtgactt tagcacccag cgccgcttcc
    63541 cacgggctaa ccggcaccac aatttccaga tcctggccga caatatcgaa cagcggatgt
    63601 ggcgcaatat gaatcaccag ccacaaatcg ccatttggac caccgttttc gcccggcgtc
    63661 ccctggcctt tcagacggat gcgttgacca ttgccgacgc ccgccgggat cttcacattc
    63721 agcgttttcg ggatttcctg ttcgatcatg ccaaaggcgt tataaaccgg caggttatag
    63781 ctgatggtac gcttatgctc agtcagcgtt tcttcgagga ataccgccac ttcgatttca
    63841 atatcgtggc cgcgtgcggc ggggcgttga cggctctggc gggcatgctg accgaaaatt
    63901 gacgagaaga tatcgtcaaa atcttcggca ttaaaactct gaccgtcgcc atgatggaac
    63961 tgatggttaa attgcggatc gttgcgatgt tgccacatct gatcatactc agcgcgacgt
    64021 tgttcatcac ttaacacttc ccaggcttca gcgacctctt tgaagcgggc ttcggcatcc
    64081 ggttctttgc tgacatcagg atggtattta cgggccagtc gacgataggc ggtcttgatt
    64141 gtcttgagat cgtccgtcgg tttcacgccc atgatggcgt aataatcctt taattccata
    64201 gcgttatctc gcgtaaatca acacaaattg aaggaacccc tgtaaggtaa ctcctataag
    64261 tgtagggtaa tcctcaaaat ttcatatgcc aacacagaat atgttattga aatcatcgcg
    64321 gagaggaggt cgccatcaag atgggttgct gaacatattt taaacaggtg aaaaagggtg
    64381 agcgattttt gatagttgaa ccaggcactt taagtttaac tagggcgtca ttatttatta
    64441 aattttatag acgctatata tgggtagtaa tatacatgga attagttgca ctgcaaataa
    64501 ttatttgaaa caggcctgga acgatataaa aaatgagtac gaaaaaaatc aaacatattc
    64561 aatcacgctt tttgaaaaca cactggtgtg ttttatgcgg ttatacaatg aactcagacg
    64621 taaagtaaat gaagaggata ctccatgtct ggaatgtgaa tcactagaaa aagaatttga
    64681 ggaaatgcag aatgataatg atctatcatt atttatgaga acattgcgta ctaatgatac
    64741 acaaatttat tcaggggttt caggaagtat tacatatact atacaatatg ttcaaaatgt
    64801 tgatattgtt agagtgtcct tgccgggcag aggttcagag tctatcacag attttaaagg
    64861 ttattattgg tatggcttta tggaatatat tgaaaacatt actgcgtgtg atgatatttt
    64921 ttctgagtat tgtttagatg atgaaaatat gagtatccag ccagaacaga taaatacgcc
    64981 gggaatatct gatttagata ctggttttaa aagaacatga gtattcatgg gcgctgggcg
    65041 gccataagac gcaaaagcaa gtatacgaag agtttattaa aattgttgat aaacaaacga
    65101 tcgaggctct tgttgatacg gcattctata atctcaggga agatgtaaag acgttatttg
    65161 gcgttaatct acaatctgac aacaaaattc aggggcaggg tggtttttta tcatggagtt
    65221 ttttgtcttt taaaaaagaa tttattgata gttgcttgaa tgacaaaaaa tgcatactgc
    65281 atttacccga atttatattt aatgataaca aggcacgcct tgttttagac accgacccag
    65341 agcagaaggt taatgaggtg aaaaatttcc tcactgctct ttcagatagc atttgctcat
    65401 tatttattgt ttacagcaat gtggcatcaa tctccttagg gaatgaatcc ttttcaacag
    65461 atgacgacct tgagtatggg tatttaataa acactggcaa tcattatgac gtttacctcc
    65521 ctcctgaact ttttgctcag gcttacgagt taaacaataa ggaaaggaat gcgcaaattg
    65581 attttttaac tcgttatgca atttaatgac aaaggcatgt gctaaaaacc attgttatta
    65641 gcctcacttc tcttattatt ggtactattg cctctgtatt agtaacgccg ctgatattct
    65701 gtttagccac aggtgcaatt atcagcggcg tacgcgaggc aggggctaat caggcatagt
    65761 ttgcgtcaaa ccttgcctgt ttttgaagat gtatatagaa aaacaggcgt tcaacaagcc
    65821 attttgcgaa cctgttcccg gaaaaaagtc atatttctgt cacactcttt aatgattgat
    65881 aacaaaagag gtgccaggaa tgaacaaaac gctaatcgcc gcaactgtgg cagggatagt
    65941 tttactcgct tcaaatgctc aggcacaaac cgtaccggaa ggctatcagc tacagcaagt
    66001 gctcatgatg agccgccata acttacgtgc gccgctggcg aacaatggca gtgtgctgga
    66061 gcagtcgacg ccgaataaat ggccagaatg ggacgtcccc ggtgggcaac tcaccaccaa
    66121 aggtggcgtg ctcgaagtgt atatgggcca ttacatgcgt gaatggctgg cagagcaggg
    66181 gatggtgaaa tcgggggaat gcccgccgcc ggacaccgtt tatgcctatg ccaatagtct
    66241 gcaacgtacc gttgcgaccg cacagttctt tattaccggc gcattcccgg ggtgtgatat
    66301 tcctgtgcat caccaggaaa aaatgggcac tatggaccca acctttaacc cggtgatcac
    66361 cgatgattcc gccgcattca gtgaacaggc ggtggcggca atggagaaag agctcagcaa
    66421 actccagctt accgacagct accagctact ggaaaaaatc gttaactata aagattcccc
    66481 tgcctgtaaa gagaaacaac agtgttcgct ggtggatggc aaaaatacct ttagcgccaa
    66541 gtatcaacaa gaaccaggtg tttccgggcc gctgaaagtc ggcaactcgc tggtagatgc
    66601 gtttactttg caatattacg aaggttttcc gatggatcag gtggcctggg gagaaatcaa
    66661 atctgaccag cagtggaagg tgttgtcgaa gcttaaaaac ggctaccagg acagcctgtt
    66721 tacctcaccg gaagtggcgc gcaatgttgc gaaaccgctg gtcagttata tcgacaaagc
    66781 tctggtcacc gatcgcacca gcgcaccgaa aattacagtg ttggttgggc acgactccaa
    66841 cattgcctct ctgttaacgg cgctggattt caaaccgtat cagttgcatg accagaacga
    66901 acgcacgccg attggcggca aaatcgtttt ccagcgttgg catgacagca aagccaatcg
    66961 cgatttgatg aaaattgaat atgtgtatca gagtgcggaa cagttacgta atgccgatgc
    67021 gttaaccctg caggcacctg ctcagcgtgt gacgctggaa ttaagcggtt gcccgataga
    67081 tgccaacggt ttctgcccga tggataaatt tgatagcgtg ttgaatgaag cggtgaaata
    67141 acagaaaact cccccgcgag aagcggggga gtcgctggtt aaacgttttt acgttcgatg
    67201 gtctgttcgc cccaaaaaag cgaatcttta tcggccttag caaaggcttt gactaacact
    67261 tcatcgctac cttcttccca aatcttttct gccatttttt cgtcgtaccc ggcgacttcg
    67321 aaaatggcct cggctatttc cggcgacgta ttgcgcagag atgcccattc accgacgtga
    67381 tgagctttcg cttcttgagt tggcatgcgt atcctcctgt tgaagattag ccgttaagtt
    67441 taactgccag acctgcgaca tattcccctt gataacgagc aatagacagt tcttcctggc
    67501 ttggctggcg tgagccgtca ccgcctgcga tggtggttgc gccgtacggc gtaccgccgc
    67561 gaacctgtga aacgtcaaat aattcctgcg ctgcgtagcc aatggggaca atcaccatgc
    67621 cgtgatgcgc aagggtcgtc caggttgaag taatagtttg ttcctgaccg ccgccagtac
    67681 cggtggaact aaagacgctc gccagttttc cgtatagtgc gccggaagcc cacaggccgc
    67741 ccgtctggtc gaggaaggta cgcatttgac cggacatgtt gccaaagcgg gtaggtgtac
    67801 caaaaataat ggcgtcgtaa tcggccagtt cttgcggggt tgcaaccggt gcagtttgcg
    67861 ttttaccgcc tgctttttca aataattgcg gcggcatggt ttccggtaca cgcttaacga
    67921 caacttctgc gccatcgact ttgcttgcac cctcagcgac tgcgcgtgcc atcgtttcaa
    67981 tatgtccgta catggaataa taaagcacca gaactttagc catttctaac cactcctcgt
    68041 gttatctcta ttccgtagcg attcgctacc acttatttaa agataagacg tccttttcag
    68101 agtgcaaatt tcacaaccac ttatttgatt tataacaact ttcacaagca cgtaattttg
    68161 tcgcaaaatg acacattttt atctcatcgc gtttttttaa tcataagagc ggcttatgga
    68221 taattattgg agatgatatc tattctcgct aagaagctgt tgcaggatat taccaaacgc
    68281 gggtctaccc gcgtcagttc actaagctta gtcccacgta gcgaaaatat ggcagccgcc
    68341 atacgccgcg ttaattctat gcaatatgat gtctataccc agacggaggt cagtaatggc
    68401 aaaccatcga ggcggttccg gcaattttgc agaagaccgc gaaagagcat cagaagcagg
    68461 taaaaaaggt ggacagcaca gcgggggtaa tttcaagaat gacccgcagc gcgcatctga
    68521 agcaggtaaa aaaggtggca agagcagtca cggcaaaagc gacaactagc cgggctaatc
    68581 aatgacgaat gcatttttgt ctgtagctcg tcaaaaagcc atcaccgccg gttacccggt
    68641 ggttgatact gatgacaaat gtaagcttgc ctgatgcgcg atgcttatca ggcctaccag
    68701 aagattgcaa tatattgaat ttgcactgtt ttgtaggccg gataaggcgt ttacgccgca
    68761 tccggcatga acaatgcgta cgttgtcaac aatctgcacc gccgggtaac cccggcggtt
    68821 ttctgtttat ggctcctgat gaacaacttc tggcggtgga acgtcaacca accttctact
    68881 taacaacgca ttgagtaaaa tcgcgccaaa ggtggctgta ccgatccctc ccaacgtaaa
    68941 accgcccagc gtgagagcaa aatcacccgc gcccagcact aaggttactg cgaccataat
    69001 caaattaccg ttctggctta aatcgacacg gttttgtacc cagatccttg cgcctgcgac
    69061 ggcaatcagc ccgaacacaa caattgatgc accaccaatc accgcggccg gaatggtatg
    69121 gatcagcgcg ccaaatttcg gcgaaaagcc caacagcatg gcgatgacgg cagcagcaac
    69181 aaacaccagc gtcgagtaga cttttgtcac ggccatcaca ccgatatttt cagcataggt
    69241 ggtgacgccg ctaccgccga cagagccgga aagcatcgtt gccagaccat caccgacgaa
    69301 tgctcgcccc atatacgggt ccatattgcg tccggtcatc ccggcgaccg ccttgagatg
    69361 acctaagttt tccgccacca gaatcaccgc cacgggcgca atcagcatca ttgcctgact
    69421 attaaaagca ggagtggaaa aatgtggcaa accgaaccag gcagcatggc tgacgagagt
    69481 aaaatcgacg gcttttccca gccctaaaac gttggtcatc acgccataca gcagacaggc
    69541 gacaattaaa cctacgagaa tcaataaccg ctggatcatg ccacgggtaa acaccgccac
    69601 cagcccaata cacagcaccg tcattaccgc catccagcta tcaaaggccg aagccgatac
    69661 acttttcact gcgataggcg ctaagttcag gccaatcgcc atcaccaccg cacccgtcac
    69721 caccggcggc attagtcgtt caatccagcg tgtaccaatt ttcatcacca ccaggccaat
    69781 gacggtataa accagcccac aggcgataat cccgcccagt gcgatgctga tattcgggtt
    69841 aatgccctga ccgttaaagc ccgtcgcggc aatcaccacg ccgacaaaag ccgcgctgga
    69901 gccgagataa ctggggacgc gcccgccggt aataaagaaa aacagtaacg tgccgatccc
    69961 cgacattaaa atggaaagat tgggatccag ccccatcaga atcggcatta acaccgtcgc
    70021 gccaaacatc gccaccgcgt gttgaacgcc cattactgcc gtctgagcaa acggcaatcg
    70081 ttcatccggc gcgaccacgc cgctctctgt agaggtcgat tttaactgcc agtgaggaaa
    70141 accgaacatt gccatcagct gtctccttaa ggaggttaac aagcagggcg catcagcgcg
    70201 tgataactgc gatcgaacca caccagcccg tagggtgtgg tgtgacgatg aatcgcttcg
    70261 atggcgcaaa acagaatgtc gtgggtgccg acgctcacca cctggctgat gcggcagtca
    70321 aacgaaacca gagcctcttc cagttgcggg catccggtca cacccgtctg ccagcgggcg
    70381 gcggcaaagc ggtgttccat gggcgttttg ccgccaaaaa gatttgaaag cggctcctgc
    70441 ccggcgctca gtgtatttac acacagcgtt cgattttcat tgaataccgg ccagacggac
    70501 gccccacgat tcaggcacac cagtaatgtg ggcggcgtgt cggtcacgct gcagacggcg
    70561 ctggcggtga acccggcgcg cccggatgga ccgtccgtgg tgatgatatt gaccgccgcg
    70621 cccatacagg acatcgcatc gcgaaaggtt tgttgatcaa ctatgttcat agtttgctcc
    70681 ttacaacagc ccgcaggctt cttcaaagga cagacgtggc aggcgcgcaa aaagtttgct
    70741 gctatcgcca tagccgatat taatcagcag attgctcttc agcgtgctgc ccgcaaaaaa
    70801 ggcgtcgtca acgtgttgac ggtcaaaacc cgacatcggg ccggtatcca gtcccagcgc
    70861 acggcaagcg acgatcagat aggccgcctg catggagctg ttgcgaaacg ctgtttcttc
    70921 ggcaagttgt gggctggagg taaaccaact gcgggcatca ccgtggggaa acagtagtgg
    70981 taaccgttca taaaattcac tgtcccaggc aacgatagcg gtgacgggcg cggtcagggt
    71041 tttttgtaga ttgccgctgg aaagcgccgg gtgcagacgt tcttttcctt ctacagtgcg
    71101 gataaacacg atccgtgccg gagaacagtt agctgatgtc ggcccccatt ttatcggggc
    71161 ataaagctcc cgtaacgtct catcgctgac gggtgtctcc cgccagccgt tgtgagtgcg
    71221 ggcatcggtg aacagggtgc taagcgcacc tgggctaacg gcttcgttca tagcaattcc
    71281 ttacagggcg gcttcacggt gatgtaacag gctggcaagc ccgttgagta acagagcatt
    71341 aaacgtttcg gg
//
LOCUS       DAKISA010000008        70302 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.008, whole genome shotgun sequence.
ACCESSION   DAKISA010000008 DAKISA010000000
VERSION     DAKISA010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70302
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.008"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..673)
                     /locus_tag="OVW45_000536"
     CDS             complement(<1..673)
                     /locus_tag="OVW45_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
                     /protein_id="HCV5525061.1"
                     /translation="MRNKLSFDLQLSARKAAIAERITAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRH"
     gene            complement(695..2707)
                     /gene="hyfR"
                     /locus_tag="OVW45_000537"
     CDS             complement(695..2707)
                     /gene="hyfR"
                     /locus_tag="OVW45_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HCV5525062.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGCRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNSCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSHLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGLSVREVL"
     gene            complement(2737..3150)
                     /gene="hyfJ"
                     /locus_tag="OVW45_000538"
     CDS             complement(2737..3150)
                     /gene="hyfJ"
                     /locus_tag="OVW45_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCV5525063.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(3143..3901)
                     /gene="hyfI"
                     /locus_tag="OVW45_000539"
     CDS             complement(3143..3901)
                     /gene="hyfI"
                     /locus_tag="OVW45_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCV5525064.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVISSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(3898..4437)
                     /gene="hyfH"
                     /locus_tag="OVW45_000540"
     CDS             complement(3898..4437)
                     /gene="hyfH"
                     /locus_tag="OVW45_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCV5525065.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASICPECKQ
                     RATLLNDDTDVPLVAKEQL"
     gene            complement(4447..6162)
                     /gene="hyfG"
                     /locus_tag="OVW45_000541"
     CDS             complement(4447..6162)
                     /gene="hyfG"
                     /locus_tag="OVW45_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HCV5525066.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDTETYPFINEGNSDARVIPIGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(6152..7732)
                     /gene="hyfF"
                     /locus_tag="OVW45_000542"
     CDS             complement(6152..7732)
                     /gene="hyfF"
                     /locus_tag="OVW45_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCV5525067.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHILNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(7737..8387)
                     /gene="hyfE"
                     /locus_tag="OVW45_000543"
     CDS             complement(7737..8387)
                     /gene="hyfE"
                     /locus_tag="OVW45_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCV5525068.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNISEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(8399..9751)
                     /gene="hyfD"
                     /locus_tag="OVW45_000544"
     CDS             complement(8399..9751)
                     /gene="hyfD"
                     /locus_tag="OVW45_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCV5525069.1"
                     /translation="MGVLFAALTTLCMLSLISAFYQADKVAVTLTLVNVGDVALFGLV
                     IDRVSTLILFVVVFLGLLVTIYSTGYLTDKNREHPHNGTNRYYAFLLVFIGAMAGLVL
                     SSTLLGQLLFFEITGGCSWALISYYQSDKAQLSALKALLITHIGSLGLYLAAATLFLQ
                     TGTFALSAMSELHGDARYLVYGGILFAAWGKSAQLPMQAWLPDAMEAPTPISAYLHAA
                     SMVKVGVYIFARAIIDGGNIPHVIGGVGMVMALVTILYGFLMYLPQQDMKRLLAWSTI
                     TQLGWMFFGLSLSIFGSRLALEGSIAYIVNHAFAKSLFFLVAGALSYSCGTRLLPRLR
                     GVLHTLPLPSVGFCVAALAITGVPPFNGFFSKFPLFAVGFALSVEYWILLPAMILLMI
                     ESVASFAWFIRWFGRVVPGKPSEAVADAAPLPGSMRLVLIVLIVMSLISSVIAATWLQ
                     "
     gene            complement(9727..9837)
                     /locus_tag="OVW45_000545"
     CDS             complement(9727..9837)
                     /locus_tag="OVW45_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoin racemase"
                     /protein_id="HCV5525070.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGFCSPH"
     gene            complement(9854..10801)
                     /gene="hyfC"
                     /locus_tag="OVW45_000546"
     CDS             complement(9854..10801)
                     /gene="hyfC"
                     /locus_tag="OVW45_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCV5525071.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEDAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(10812..12830)
                     /gene="hyfB"
                     /locus_tag="OVW45_000547"
     CDS             complement(10812..12830)
                     /gene="hyfB"
                     /locus_tag="OVW45_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HCV5525072.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFEFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(12830..13447)
                     /gene="hyfA"
                     /locus_tag="OVW45_000548"
     CDS             complement(12830..13447)
                     /gene="hyfA"
                     /locus_tag="OVW45_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCV5525073.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(13700..14170)
                     /gene="bcp"
                     /locus_tag="OVW45_000549"
     CDS             complement(13700..14170)
                     /gene="bcp"
                     /locus_tag="OVW45_000549"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCV5525074.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(14170..14742)
                     /locus_tag="OVW45_000550"
     CDS             complement(14170..14742)
                     /locus_tag="OVW45_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCV5525075.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            14888..15766
                     /gene="dapA"
                     /locus_tag="OVW45_000551"
     CDS             14888..15766
                     /gene="dapA"
                     /locus_tag="OVW45_000551"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCV5525076.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            15783..16817
                     /gene="bamC"
                     /locus_tag="OVW45_000552"
     CDS             15783..16817
                     /gene="bamC"
                     /locus_tag="OVW45_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCV5525077.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            17030..17743
                     /gene="purC"
                     /locus_tag="OVW45_000553"
     CDS             17030..17743
                     /gene="purC"
                     /locus_tag="OVW45_000553"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCV5525078.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            17911..18759
                     /locus_tag="OVW45_000554"
     CDS             17911..18759
                     /locus_tag="OVW45_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCV5525079.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGDPAQCN
                     TFGKSI"
     gene            18774..20789
                     /gene="tmcA"
                     /locus_tag="OVW45_000555"
     CDS             18774..20789
                     /gene="tmcA"
                     /locus_tag="OVW45_000555"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCV5525080.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSDPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNTSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            20863..21561
                     /gene="ypfH"
                     /locus_tag="OVW45_000556"
     CDS             20863..21561
                     /gene="ypfH"
                     /locus_tag="OVW45_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCV5525081.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIEMVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(21671..21871)
                     /locus_tag="OVW45_000557"
     CDS             complement(21671..21871)
                     /locus_tag="OVW45_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCV5525082.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(21899..23026)
                     /gene="dapE"
                     /locus_tag="OVW45_000558"
     CDS             complement(21899..23026)
                     /gene="dapE"
                     /locus_tag="OVW45_000558"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCV5525083.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIDRLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAETGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(23030..23386)
                     /locus_tag="OVW45_000559"
     CDS             complement(23030..23386)
                     /locus_tag="OVW45_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCV5525084.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            23768..23827
                     /gene="ypfM"
                     /locus_tag="OVW45_000560"
     CDS             23768..23827
                     /gene="ypfM"
                     /locus_tag="OVW45_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCV5525085.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(23926..27039)
                     /gene="acrD"
                     /locus_tag="OVW45_000561"
     CDS             complement(23926..27039)
                     /gene="acrD"
                     /locus_tag="OVW45_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCV5525086.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGIGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(27203..28903)
                     /gene="narQ"
                     /locus_tag="OVW45_000562"
     CDS             complement(27203..28903)
                     /gene="narQ"
                     /locus_tag="OVW45_000562"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HCV5525087.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSSVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            29109..31088
                     /gene="aegA"
                     /locus_tag="OVW45_000563"
     CDS             29109..31088
                     /gene="aegA"
                     /locus_tag="OVW45_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCV5525088.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDVQGRRRLVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            31156..31731
                     /gene="nudK"
                     /locus_tag="OVW45_000564"
     CDS             31156..31731
                     /gene="nudK"
                     /locus_tag="OVW45_000564"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCV5525089.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            31857..32900
                     /locus_tag="OVW45_000565"
     CDS             31857..32900
                     /locus_tag="OVW45_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCV5525090.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAALAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(32996..34999)
                     /gene="tkt"
                     /locus_tag="OVW45_000566"
     CDS             complement(32996..34999)
                     /gene="tkt"
                     /locus_tag="OVW45_000566"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCV5525091.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQRWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPLLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEITRGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(35019..35969)
                     /gene="tal"
                     /locus_tag="OVW45_000567"
     CDS             complement(35019..35969)
                     /gene="tal"
                     /locus_tag="OVW45_000567"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCV5525092.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQYAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            36258..38537
                     /gene="maeB"
                     /locus_tag="OVW45_000568"
     CDS             36258..38537
                     /gene="maeB"
                     /locus_tag="OVW45_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCV5525093.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            38831..39166
                     /gene="eutS"
                     /locus_tag="OVW45_000569"
     CDS             38831..39166
                     /gene="eutS"
                     /locus_tag="OVW45_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCV5525094.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIVGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            39179..39658
                     /gene="eutP"
                     /locus_tag="OVW45_000570"
     CDS             39179..39658
                     /gene="eutP"
                     /locus_tag="OVW45_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCV5525095.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            39633..40334
                     /gene="eutQ"
                     /locus_tag="OVW45_000571"
     CDS             39633..40334
                     /gene="eutQ"
                     /locus_tag="OVW45_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HCV5525096.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            40331..41134
                     /gene="eutT"
                     /locus_tag="OVW45_000572"
     CDS             40331..41134
                     /gene="eutT"
                     /locus_tag="OVW45_000572"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HCV5525097.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKPPLTVKQIQQRLGETQ"
     gene            41131..42147
                     /gene="pta"
                     /locus_tag="OVW45_000573"
     CDS             41131..42147
                     /gene="pta"
                     /locus_tag="OVW45_000573"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCV5525098.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRALTGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            42186..42479
                     /gene="eutM"
                     /locus_tag="OVW45_000574"
     CDS             42186..42479
                     /gene="eutM"
                     /locus_tag="OVW45_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HCV5525099.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            42586..42873
                     /gene="eutN"
                     /locus_tag="OVW45_000575"
     CDS             42586..42873
                     /gene="eutN"
                     /locus_tag="OVW45_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HCV5525100.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            42885..44288
                     /locus_tag="OVW45_000576"
     CDS             42885..44288
                     /locus_tag="OVW45_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HCV5525101.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPRIAGLGLGGEGWTTMTITTPTGEGVTSVR
                     TFVRLRRCVLVDAFRIV"
     gene            44299..45135
                     /gene="eutJ"
                     /locus_tag="OVW45_000577"
     CDS             44299..45135
                     /gene="eutJ"
                     /locus_tag="OVW45_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HCV5525102.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQSVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            45125..46312
                     /gene="eutG"
                     /locus_tag="OVW45_000578"
     CDS             45125..46312
                     /gene="eutG"
                     /locus_tag="OVW45_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HCV5525103.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            46629..47855
                     /gene="eutH"
                     /locus_tag="OVW45_000579"
     CDS             46629..47855
                     /gene="eutH"
                     /locus_tag="OVW45_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HCV5525104.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATTAKTEAEAQS"
     gene            47852..49255
                     /gene="eutA"
                     /locus_tag="OVW45_000580"
     CDS             47852..49255
                     /gene="eutA"
                     /locus_tag="OVW45_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCV5525105.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMVLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            49267..50628
                     /gene="eutB"
                     /locus_tag="OVW45_000581"
     CDS             49267..50628
                     /gene="eutB"
                     /locus_tag="OVW45_000581"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HCV5525106.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNRSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            50649..51536
                     /gene="eutC"
                     /locus_tag="OVW45_000582"
     CDS             50649..51536
                     /gene="eutC"
                     /locus_tag="OVW45_000582"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HCV5525107.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRTGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            51546..52205
                     /gene="eutL"
                     /locus_tag="OVW45_000583"
     CDS             51546..52205
                     /gene="eutL"
                     /locus_tag="OVW45_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCV5525108.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            52218..52718
                     /gene="eutK"
                     /locus_tag="OVW45_000584"
     CDS             52218..52718
                     /gene="eutK"
                     /locus_tag="OVW45_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCV5525109.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            52764..53816
                     /gene="eutR"
                     /locus_tag="OVW45_000585"
     CDS             52764..53816
                     /gene="eutR"
                     /locus_tag="OVW45_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HCV5525110.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(53822..54721)
                     /gene="hemF"
                     /locus_tag="OVW45_000586"
     CDS             complement(53822..54721)
                     /gene="hemF"
                     /locus_tag="OVW45_000586"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCV5525111.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(54725..55594)
                     /gene="amiA"
                     /locus_tag="OVW45_000587"
     CDS             complement(54725..55594)
                     /gene="amiA"
                     /locus_tag="OVW45_000587"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCV5525112.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            55808..56233
                     /locus_tag="OVW45_000588"
     CDS             55808..56233
                     /locus_tag="OVW45_000588"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCV5525113.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            56220..56669
                     /locus_tag="OVW45_000589"
     CDS             56220..56669
                     /locus_tag="OVW45_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCV5525114.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            56730..57305
                     /locus_tag="OVW45_000590"
     CDS             56730..57305
                     /locus_tag="OVW45_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCV5525115.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            57401..58300
                     /gene="yfeX"
                     /locus_tag="OVW45_000591"
     CDS             57401..58300
                     /gene="yfeX"
                     /locus_tag="OVW45_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCV5525116.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(58358..59662)
                     /gene="pbp4b"
                     /locus_tag="OVW45_000592"
     CDS             complement(58358..59662)
                     /gene="pbp4b"
                     /locus_tag="OVW45_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HCV5525117.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQIDVGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(59667..59840)
                     /locus_tag="OVW45_000593"
     CDS             complement(59667..59840)
                     /locus_tag="OVW45_000593"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylglucosamine transporter subunit
                     IIBC"
                     /protein_id="HCV5525118.1"
                     /translation="MGLNSAFGPSGLVALPLMTSAQGILPAMAVYAGGILVAWVCGFI
                     FTTLFGCRNVNLD"
     gene            complement(59853..61091)
                     /gene="murP"
                     /locus_tag="OVW45_000594"
     CDS             complement(59853..61091)
                     /gene="murP"
                     /locus_tag="OVW45_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCV5525119.1"
                     /translation="MAKEISSELLNTILTRVGGLGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMSDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIA"
     gene            complement(61095..61991)
                     /gene="murQ"
                     /locus_tag="OVW45_000595"
     CDS             complement(61095..61991)
                     /gene="murQ"
                     /locus_tag="OVW45_000595"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCV5525120.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGTEDSREGGINDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            62155..63012
                     /gene="murR"
                     /locus_tag="OVW45_000596"
     CDS             62155..63012
                     /gene="murR"
                     /locus_tag="OVW45_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCV5525121.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVAYEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            63141..63932
                     /gene="ucpA"
                     /locus_tag="OVW45_000597"
     CDS             63141..63932
                     /gene="ucpA"
                     /locus_tag="OVW45_000597"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCV5525122.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            64103..65119
                     /gene="cysP"
                     /locus_tag="OVW45_000598"
     CDS             64103..65119
                     /gene="cysP"
                     /locus_tag="OVW45_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCV5525123.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWTKENGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            65119..65952
                     /gene="cysT"
                     /locus_tag="OVW45_000599"
     CDS             65119..65952
                     /gene="cysT"
                     /locus_tag="OVW45_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCV5525124.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            65952..66827
                     /gene="cysW"
                     /locus_tag="OVW45_000600"
     CDS             65952..66827
                     /gene="cysW"
                     /locus_tag="OVW45_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCV5525125.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            66817..67914
                     /gene="cysA"
                     /locus_tag="OVW45_000601"
     CDS             66817..67914
                     /gene="cysA"
                     /locus_tag="OVW45_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCV5525126.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRDRPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPEQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            68048..68959
                     /gene="cysM"
                     /locus_tag="OVW45_000602"
     CDS             68048..68959
                     /gene="cysM"
                     /locus_tag="OVW45_000602"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCV5525127.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(68962..69330)
                     /locus_tag="OVW45_000603"
     CDS             complement(68962..69330)
                     /locus_tag="OVW45_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCV5525128.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            69435..70286
                     /gene="pdxK"
                     /locus_tag="OVW45_000604"
                     /gene_synonym="thiJ"
     CDS             69435..70286
                     /gene="pdxK"
                     /locus_tag="OVW45_000604"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCV5525129.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHSDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
BASE COUNT        16700 a        18354 c        19439 g        15809 t
ORIGIN      
        1 aatggcgaat ggcaatggcg aacggaatca caaacaaatt ggcgatacag tgctcaaacc
       61 cactggcgac aaacagggtg atgggcaaaa tcatggcgac gattttgtcg cataatgaac
      121 gcccgcaata actcatccac agcgccagac aaaccatcag attgcacata atgccgaggc
      181 tgacagattc agtaaatgta tgatgcattt tgccctcggc gcagtgtaaa accgcgactc
      241 cccacatcgc gttttcactc atcaccagcc cggaaaacca gattaacaaa ctgaaacagg
      301 caatacctgc cagattaccg caggccacca gaagtgcgtt aatcagccaa gttcgccaac
      361 taataacgcc ccgacttttt gccatcaccg tcattaccga cgaggtgaac aggctggtgc
      421 cacaaaccgc cagcaaaata aagccgagtg taaagcaaag gccgcccacc agatgggtta
      481 atgcctgtga agacggggca tcggcgataa cggaaaggta aaaagtaaat ccaatcgcca
      541 taaacacgcc agcggacatc gccattaaaa agaccgacac tttgctgcgg gcaattttat
      601 gggcagtaat ccgttcagcg attgccgctt ttctggcgct caactgcaag tcgaaagaga
      661 gtttgtttct catgcatact cctgcaaaag cagattacaa cacctcgcga accgagagcc
      721 ccagacgctg cattcgtgac agcagcgtgg tgcgcttcat ccctaatcgt gtcgccgcgc
      781 cacggggccc ggcaacaatg ccattggttt ctcgcaatac ctgaacaatg cgctgacgtt
      841 cttcttcgtc attctctggc gtcgtcgggt gcagcaactg cgccattgaa ctgcgaagcg
      901 ctgaatcttc atttagcgtc ggcagtaaat ggctttgtcg gacattaaga tgtaaattca
      961 gactgttacc gcgagtcagc agtaccgctc gctcaatcac gttttccagc tcgcgcacgt
     1021 tgcccggcca gtcccacgac atcaactggc gtagcgcctc ggtcgggatg gcgtcaattg
     1081 aacgattcat atggcgcgcc attttttgcg tgaaatgctt tgctaaaaga gggatatctt
     1141 ccggacggtc gcgtagcggc ggcaattcca gtgggaagac attcaggcga taaaagagat
     1201 cgctgcgaaa ctggcgatct tcaaccattt gccacaaatc acggttggtg gcggcaatga
     1261 ctctgacatt caccgggatc gttctactcc cgccgagacg ctcaatctcc cgctcctgca
     1321 atacgcgcag cagtttaggc tgaagttcta acggcagatc gccaatttca tcgagaaaca
     1381 acgtgccgcc atcggcaatt tcaaaacgac cacgatgggt attaatcgca ccagtaaacg
     1441 cccctttgtc atgaccgaat aactcacttt ccagaagact ggcggggatg gcagcgcagt
     1501 tgattttgac cagcggcttg tcgcgtcgcg ggctaagttg atggatcgct ctggcgatca
     1561 cctctttgcc ggttccggtt tcaccacaaa tcaacactgt actgtcgctc ttcgccacaa
     1621 tatctacctg ctggagcagg tcttccattg cctggctttg atagataatg tcaccgacgc
     1681 ccagattcga aaggagctgc tcgctaagct ggtggttttc ttgctgcaaa ctttcctgca
     1741 aatcggtcat gctacgccag gcatcggcat tgccaacggc aatagcgatg cgatcggcta
     1801 tgtgttgtag tagctgacag ttttcctcac taaagagagt ggaagaggta tgcgccagca
     1861 acaatgcacc cggtgtatgg ttgccaaagg taagcggtat aaggagcgca gattcgcagc
     1921 tgttagatgc aagtaagagt aataacgggt cgcgttgcca gagaaacaga tcgtctgcac
     1981 ggtgcgtcaa ggtcggttgt cgggtttgta gcgtttgtgt cagcaataca ctgtcgccag
     2041 gcatattgcg tggcagacac gcacaatgtg aggcagaaag atcgctgcac cacaggctaa
     2101 acttctcgtt ctttcgatga tcgcccagta ccatactgac tgaagccaga ccgaaaaaat
     2161 gatggatctc acgagcgacg tcagcgatca gatcgtcgag atcaagatgt gacagcactg
     2221 cattagtaat atcaaccagt acccgatgat gatcgcgatc tttctccagc aaacgcagcg
     2281 ccacctgctg ttcttcatca ttgagccatg attgcagaac cggggtgatt atccgcgcca
     2341 gccagttaaa cagtgtgcaa tcctggggag taaaacggca gccgtgggtg cgaataaagc
     2401 gtagctccgc cattccacta ctgccgagaa ggacggcgtg acgcgaataa tcgaccatct
     2461 cgccctgata ctctgttgtt gccgtcacat cgctgccatc atcatggtag cggtaataga
     2521 agttgctttt gagaacatgc agttcaataa gttgtaccgg gctgaacggc ggcggcagcg
     2581 ggatgaaggc gcgtaataaa gacgccttac cgtgtttctg cgctaaccgc tccgcgagca
     2641 ttccgtttac cgcttcaatt gttattcctt gcggcggggc aaacatcgcc tcgtctgaca
     2701 tagccatcaa cagtccttat taatgagaaa tccttctcac gcaatttttc tggccatcag
     2761 atagatggcc ggttcctgca caatttcatc caacagatga atgaattgaa tgctacgcgc
     2821 ctgccacaca gacgtcgcgt catccgccag tggcgcgaat gcccgggtca acagggcggt
     2881 gtggctggcg tcaataacaa tctcaccaaa agtcatcacc cccagcatct tacgtcgggc
     2941 ttgctcctct tcgaccagtg caagccaatc ttcgtattcc gtcagtgggc aggggaaggc
     3001 gacattcaga caatcaatca cgccaacgtg atgaccgata gccagcgagt aatacattac
     3061 ctgagagctg tgttccggca tctcgtcgct actggcgaca aacttttttc gcagcgtcca
     3121 gaaaacaatt tccccgcact cttcagtcat gtaacccccg gagtacgcga aagagttgct
     3181 gaacgatact attgagacgt ggatcgtcgg catcgcgcaa ccaggtgtta acccgatttc
     3241 ctgtaggatc gtcgcttaaa tggcgcagga gccgatcgca aatttctcgc ccctgacgat
     3301 agcccgccag ccgccgcgct tctcgctcaa tggcgatacg ctgtgatggc gggatctgcg
     3361 gccacaacgg ttgcatagtc accccggtgg gatcgcgata atccaccgcg tgaatcttct
     3421 gttgcagcaa accgagcgcc acggcgaaac cgtgaatggt ggcggccggt gttggcgggc
     3481 agccggggat ccaaacatca atggggacaa tggtgtcgct gccgccccag acgctgtaga
     3541 gatcgtggaa aataccgccg ccgacaccgc acgcgccgta ggaaacacaa attttatgat
     3601 cgggggcaga ctcatacgcc cgaagtgcag gcatacgcat cgcccgggtg actgcgcctg
     3661 taaataacaa aatatcggcg tgacgcggtg atgaaataac cttaatgcca aaacgttctg
     3721 cgtcgaatac tggtgtgatg gcggcaaaaa tttcgatttc acaggcgttg cagccgccgc
     3781 aatcgacgcg ataaacgtaa gccgagcgac ggatatcctg tagcaaatgc tgcttcatct
     3841 tttgcgtttg ctcgtccagc gtgatgggct ggctgacatg ttgtgtaagc actggactca
     3901 tagctgctcc ttagccacca gcggtacatc tgtatcgtcg ttgagcagcg tcgcgcgttg
     3961 tttgcactcc gggcagatgc tcgcttgcgc ccataacatt tcgcggtttt gtggcgcatt
     4021 ttgttgctgt gccaacaatt cagcagccag tgcgacggtt ttttgcgggg caaacgggcg
     4081 ttcgcaacgg ctgcaacgtt gtagatggaa cgtcgcgcgg gtatagagat cggctttatt
     4141 ggtgacggtc agttcaaagt tattggtaag ctggatggct ctggtcgggc acacttcttc
     4201 acaacgtccg cagtaaatac aacgcctcag atagagctgc caggtgcgcg aattttgctg
     4261 gtcgtcggtc tggatagtca gcgcatttgc cggacaagca caggcgccgc aggcaataca
     4321 ttggctgggc atcaggtccg gtttcccgcg aaagcctggg ctgacctcca gtggcgcgaa
     4381 gggatatttc accgtcgcgg ttccggcgcg cataatagtt ttcagtaact tcagcatctt
     4441 ctgtccttac ttcagcggcg aacggttacg atcaatgccg tagcgttcga tctctttata
     4501 cggcacggtt tttgactggc gcttgcgcac atctaccagc gtcacacggt cggtacagga
     4561 gtagcaggga tcaaggctac cgataatcag cggtgcgtca gaaacggtat tgccgcgcag
     4621 catgtaacgc aacaccggcc agttggcgta ggtggcggca cggcagcgcc agcggaacaa
     4681 tttttggtta tcaccgagca tgctccagtg cacgtcttca ccgcgtggcg cttcaacaaa
     4741 gcccagcgcg aatgcgtgag gtttatagct aaagccttcg gtcagcagtg gggtatccgg
     4801 catgttgtcg agggcaaatt ccagcattgc cagcgaatca aacgtctctt tgacacggac
     4861 catcacgcgg gagaaaacat cgccgccggt aaaggtaaac agtgtttttg gaatattgcc
     4921 gtagtcggcg taggggtgat caaagcgcaa atcacgggca aaaccactgc cgcggatcag
     4981 cggccctaca gggctataat cacgggcgat ttgtcggtcg agaatgccaa tgccctgagt
     5041 gcgttgttcc atattcggcg tggcaagcag catctctacc agctccgaca cgtcggcgcg
     5101 catctcgcgc accagtttca gcgtttgcag acgttgctct ttgagaatat cgcggcgaac
     5161 accaccaatc agattcagac cgtaggtttt acgcgacccg gtcagcaatt ccgccattgt
     5221 catcgacttt tcccgcacgc ggaaaaattg cataaagccg gtatcaaatc caacaaaatg
     5281 gcaggagagg ccaaggttga gcaaatgact gtgcagacgt tcgacttcca gcagaatcga
     5341 gcgaatagta tgcgctcgtt gcggcacctc aatccccagt gcattttcaa ccgagttggt
     5401 ataggccaca ctgtgggcaa aaccgcaaat cccacacacg cgatccgata agaaggtcac
     5461 ttcgttgtag cccatccgcg tttctgccag tttctccatg ccgcgatgga cataaaacag
     5521 gcggtaatca gcatcgacaa tttgctcgcc atccacaaac aagcggaagt gacccggttc
     5581 atcggaggtg atatgcagcg ggccaatagg gatcacccgc gcatcgctat tgccctcatt
     5641 gataaacgga tacgtttcgg tatcagtcgt cggttcaggg cgcagtcgat aatccatcgc
     5701 atctttgcgc agcggatgca tatcttccgg ccagtcatcg ggcaacacca ggcgacgctg
     5761 atccggcagg ccaaccggaa tcagcccgta catatcgcga atttctcgct cgccccagac
     5821 cgcggcaggg acgcgcggtg tgactgacgg aaactcccga ctgtcggcat cgaccagtgc
     5881 cttcaccacg atccagcatt tttcggcccc ttccattgaa agggcataat aaaccgcgta
     5941 atgaccgtta agtgtccgct cgtcgttgcc aaacaggact ggaagccagc catcatgttg
     6001 ataataaaga taatgtacaa cgtcaggcag cagattgatt ttcaccgtaa tggtgacctg
     6061 ttcaggcgtt tgtcgctctt catccagcac cgcgccgggg aactgcgttt tcagggcggc
     6121 gagaatcgct tcgccacgat ttgacgatga attaacgttc actgtgtttc tccgaaacag
     6181 atgcggtgcc tgaaggcaaa aaatcatgcc aggtgctacg ttgtgcaggc aggtcgtgcg
     6241 tccctgagag gactatagtg gaagcgcccg ccaggatcct gatgacaggt tgtggaatat
     6301 gcgttcccat cgccagcatc atgaccagca gaatcaccat tggcgaggtg gtcaaccagc
     6361 cgagatcacc ccggttaacg gcctgcggcg gtttcgccat taacacccgc gctgccatcc
     6421 gtaccaggcc cgccagcacc agcgttaaca gcaataacag caggacgata atcagcaggt
     6481 gattacgtgc cagtccggcg gtaatggtca taaattcgct aagaaaaatg ttgaagggcg
     6541 gcatccctgc cagcgccagc gcaccgccgc caaacagcac ggcggtaaat ggcatgattt
     6601 tgagcatccc acagacgacg ttgagatcgc gcgtgccgta cttgagcagt acattgccgg
     6661 aaccgcagaa cagcagcgtt tttgccagac tgtggtttaa gatgtgcagc agcgcggcaa
     6721 aaattcccag cggcccgcca atgcctagcg ccaccgcgac cagccccatg ttctccacgc
     6781 tggagtacgc cagcagacgc ttaatgtccc gctgtaccag aatgaaaaat gccgccacgg
     6841 caaccgacaa catgccgaag atgagcaaca accggttggg gaaatcgctg ccgatggctt
     6901 ggcaaataat gatgtaatag cgaatcagca ccaacagcgc gcagttcagc aatacggcgg
     6961 agagcagggc gctgaccgga ctcggcgctt cactgtgagc atccggcagc caggcgtgca
     7021 tgggaaatag cccggttttg gtaccaaagc caattagcac aaacacaaag gccaacagca
     7081 ttaatgttgg gtcaagcaag gacgattgct taagaacctc gctccagaat atcgccattt
     7141 ctgcctgcgg cataacgctg gcggcgttgg cgtataccag cacggtaccg aacagaccaa
     7201 aagcgacacc aacagtacaa ataatgatgt acttccatgc tgattccagc gatgaacgct
     7261 gaccgtaaat gcctaccaga aacgccgagc ttaaggtggt ggcttcgatc gccgcccaca
     7321 tcacaatcag gttattgctg gtaacaacca gcagcatggt gaacaaaaac agatggaaga
     7381 aaccgtagta atcgcacagc gtaacgggtg aaagctcgcc gtgtgccact tcatgacgca
     7441 tgtagccaat cgagtaaaca ccggtgagaa agccaatcac accaagaatg gcgaggaaca
     7501 aaccgcccag accatcaata tgaagccaca gtcccgcagc gaatatttct cctgcatcag
     7561 cggcagtttg gaccacccag agtgccagaa tcagcagcag tgtgatccct aagctatgta
     7621 atacagtcac cgtacaagtc gcagagagcc cccgtttccg gcaggcaaaa cagagcagcg
     7681 aaaaaagcag cggcgtgagc aggagtaaag cgaacatcac agaataactc atctcattat
     7741 cccttcagcg cggtcaagtt gttcacgtcc agcgtgccgt gggtacgcca tatttttctt
     7801 gccagtaaca ccatcacaat gacggcgaag atggcgtcgg tagcgatacc tatttccacc
     7861 agttccggtg ctcgccaggc aagaagcgcc agcaccagat gggagccgtt ttccatcagg
     7921 cagtaaccaa aaatttgccg caggatattg cgctggctga caatgcacag caggccaagc
     7981 agaaaatgac ctaacgctac cgccagcgcc ggtttcagcc cggtagccat cggtagcttc
     8041 acgggctgaa cgacaaatgc gcaaagcagg acaattaacg ccgcgagcag tgccatcatt
     8101 gccggaccga ataacgcttt ttccgagatg ttctggggaa tatttcgtgc agcgtaagtc
     8161 atgattaacg gtaccagcag cactttggtg ataaaggcgc tggcggacca gatcagcagt
     8221 tgctctgcgg cgaacaggca cgagagagtg gcgaaaatag acaccagcac cagcgactgg
     8281 caggcgtaaa atccgcatga caggcgatag cttttgacgc taatcacaaa cagcgatgtc
     8341 agcatcatca gtcccgccag attatttacg atcatagaac cggtcattca tcatctcctt
     8401 actgcaacca ggtcgcggcg attacgctgg aaatcagcga catcacaatc agtacaatca
     8461 acaccaggcg cattgaccct ggcagcggtg cggcatcggc gacggcctcg ctcggtttgc
     8521 ccggcacgac gcgaccaaac cagcgaataa accaggcgaa actggcgacc gattcaatca
     8581 tcagcagaat catggcgggc agcaggatcc agtactccac tgacaacgca aaaccgacgg
     8641 caaacagcgg gaatttactg aagaagccgt tgaacggcgg cacaccggta atcgccagcg
     8701 ctgccacgca gaaacccacg cttggcaacg gcagggtgtg caatacgcca cgcagacgcg
     8761 gcaacaagcg cgtgccgcag ctgtaactca gcgcacctgc tacaaggaaa aacaggcttt
     8821 tagcgaacgc gtggttgacg atgtaggcga tgctaccctc cagcgccagc cgcgagccga
     8881 agatggagag cgacaagccg aagaacatcc agccaagttg agtgatggtc gaccaggcca
     8941 gcaaccgctt catatcctgc tgtggcaaat acatcagaaa gccataaaga atggtgacca
     9001 gcgccatgac catgccaacg ccgccaatca catgcgggat attgccgccg tcgataatgg
     9061 cgcgggcaaa aatgtaaacg cccactttca ccatcgatgc ggcgtggaga taggcactga
     9121 tcggtgttgg cgcttccatt gcatccggta gccacgcttg catcggtagc tgggccgatt
     9181 tcccccacgc ggcaaacagg atgccgccat aaaccagata acgtgcgtcg ccgtgtaact
     9241 cgctcatcgc gctaagcgca aacgttccgg tctgcaaaaa cagcgtggcg gcggcaagat
     9301 acaaccccaa cgagccgata tgagtgataa gtaacgcttt tagtgctgaa agctgtgctt
     9361 tatcgctctg gtaataactg atcaacgccc aggagcagcc gcccgtaatt tcaaaaaaca
     9421 acaactgacc gagcagcgtc gaggagagta ccagtcccgc catcgcgccg ataaacacca
     9481 gcaaaaatgc gtaataacga ttcgtgccgt tatgcgggtg ttcgcgattt ttatccgtca
     9541 gataacccgt ggagtagatc gtgaccagca aaccgagaaa caccaccaca aacagaatca
     9601 gcgtactcac gcgatcaatg accaagccaa acaatgccac atcccccacg ttgaccaacg
     9661 tcaacgtgac ggcaacttta tcggcctgat aaaacgcgga gatcagcgac aacatgcaca
     9721 gcgtggtcag tgcggcgaac aaaaccccca ttcggcggcc cgacgttgtg gcgaaaacga
     9781 aacgaccagt gcgccgataa aaggcagcat taacgtcgtc agagcaagat tttccatatt
     9841 ccgtcagtgc tccttacaga ccggttaacc agaagaccca tgcaagcgca gcaaggctga
     9901 agccaagcca ggtcacatgg tgaatgagta aaaaacgccc gcgtgccagc gtgttttctg
     9961 cgattgaggc cagtacaaaa atcagcaaaa ccttgagcag cgtaacgaca agtgaagtca
    10021 gcaggcaggc gagagaaagt tcttgcgcgc gcccaaaggg cagaaacagg gccacaaaca
    10081 gtgctgccat cacgacctgt ttcagcccca gcccccattt cgctagcgcc agcccggcac
    10141 cggaatattc ggtcagcggg ccttcctgta attcctgttc tgcttcagca acatcaaagg
    10201 gaatttttcc catctcaatg aagcaggcaa aaccacaggc cagtaacgcc agtacggtgg
    10261 ttagcggcga gttccagccc gttgccagcg tattgctgat catctcgata tgcgtggaac
    10321 ctgctatcag cgccaatacc agcagtgaga gaatgagcat gggttcgacc agaatgccga
    10381 gcgtcaactc gcgactggca ccgactcccg caaacgggct tccggtatcc agccctgaaa
    10441 gagcaaagaa aaaacgaaac agggcaagaa gatagataag ggtgatcaga tcgccgccgc
    10501 ccgcaaaagg ggaaacggta ataaatagtg gtagggccat cgccagcacc agcatgctgc
    10561 tgattaatac ccacggcatc aggcggaaca tcagacctga agatgtcggc gcatcttcct
    10621 ggcgtttaaa cagtttgtgg atatcgcgat aatcctgcca gatccccggc ccgcggcggg
    10681 agtgcatacg cgcgcgtatc tgccgggaaa tacccgtaaa aagtggggtt agcatcagca
    10741 gaatcacggc ctgtgctaac gcaaaaatga ccagatatcc gtcgcaaaga gtttgtctca
    10801 tggtgatttc cttagacggc aatagcgatt agcagcacaa ccagtgcggc gaccacatac
    10861 aggcaataga gacgaaagtc gccgctttgc agatgctgta tttctttggc cagccgttgg
    10921 gtggcgctca cgatggggcg gatcacccgc tcatcccaga agggttctgt gctctgagcc
    10981 ctggcggtga cgtgcgcaag acctttattt agcctcagcg tagggtcgag ctgttgtcgt
    11041 agacgaaata gcgcagaaaa gaccacacgc agcggctgca tcacgccatt gcctgacggg
    11101 gccatcgcat tttcccagcc gtagccgcat gcccaggcat ctcctgtgcg acgaaacgct
    11161 gcgcggcgcg aacgacacca cagccagtaa aggccaggca gcaaaggcat cgccagtagt
    11221 aacagcaaca gtaatgaagg agtgacccgt gtatgaaacg tgccgggtac aagtgctatt
    11281 ccgctggcga cagtgacggg aggggtattg gtaaacgcat gggcaatgtg cattatcttc
    11341 ggtgccagcc aactggcact aagcgcaatt aatacgcaga gtgccgcgag cagtagcatg
    11401 gcgacgatca tcgtatttgg cacttcctgt gcctcttcag cgtgtgtgct gcgcggcgca
    11461 ccacagaaag taataccgta cattttgacg aagcacatta ctgccagccc accagtgact
    11521 gccagcatta ccatagcaat aggacccgca agttgtagcg ctacggcttc cacacgactt
    11581 agtgagaata gcgactgcca ggtgtaccat tcgctgataa aaccattcag cggaggaatg
    11641 gctgatatcg ccaggcaacc aatcaggcat gctgcggctg tccacggcat ccgtttcgcc
    11701 agtgccccca ttttttccat gtcgtgggta tgcaaacgcg aaataatcgc acccgcgccg
    11761 agaaatagca gccctttgaa aagcgcatgg ttgagcagat gaaacagtgc gccgagcagt
    11821 ccaaccacgg tgagcagcgg gtcgtgcagt gacagaccga ccatcgccac accgaccgcc
    11881 agcaaaataa tgccgacgtt ttcgacggta ctccaggcca gcagccgttt gatatcctgt
    11941 tccgccagtg catacagcac gcccaggagc gcggagattg cgccgatcgc catcaccaga
    12001 atgccccacc acagcggcaa acccgtttgc gccagcagat ccatcgccac tttcaggatg
    12061 ccgaaaatac cgattttgac cattacgcca gacatcaacg ctgaagcgtg cgatggtgcg
    12121 gcagggtgag cgcgcggcaa ccagctgtgc aacggcatca tcccggcttt cgcgccaaaa
    12181 ccgaaaaagg ccagcaggaa caccgccgac gccagccccg gagaaagtga aagcgtgcgg
    12241 aaactggcaa aatcgaggct gccgctttcg cgccccatca gcaagaaggc gatcattatc
    12301 agcaccgatc cggcgtgggc gataaaaaag tagagcatgc cagcgttgat cgacgtttta
    12361 tcctgcctgg caatgaccag aaaccaggaa gacagcgaca tcatttcaaa cagcacgatg
    12421 aaccaaaaag cgttgtccat caccagcagg gcaaccatcg atgcgatgaa aatattcata
    12481 aagaagccca tcgccgccgc gcctttgccc tcgtattcgc gcatataagt caatgaatag
    12541 agagaacaaa ccaccaccag caaggagata accagcacca taaatgccga gaggctatcc
    12601 attcgcaggg ttaaatcggc aaattcaaaa ggcgtggcaa aacgggcgac taaagttacg
    12661 cctgcatgta attgcgtaat tccgctgatg atgccaatca cgccgcccac cagcgatgtg
    12721 atgccggaaa gcttaatagc cagtctgtcc agaccgagta aaaacagcga agccagactg
    12781 gcaaacagat agaggatcag cgaccaggtt aataattgca gggcatccat tagcgttgct
    12841 cctgagtgag ggaaagggga tcctccccgc cattggcaaa ccagcttgcg gcaaggcgct
    12901 gtttttcttt catctggcgt tgcaggctat cgtcggtgat cagccgtaac gcctgattcg
    12961 ggcaagcgcg aacgcaggcc ggaccttctg gcaagaaatc acacaggtcg catttcaccg
    13021 cgacggtctg gacacccggt tcccagcgca gcaacgcatg ttggggcggc acgttttctt
    13081 cgccgtcttt gagtgagccc tcagcctgaa aaacatattg cgcaggggca ttcaccggac
    13141 ggcttcctga agcgctgatt gcgccaaatg ggcagaccac ggcgcaaagc ttgcagccaa
    13201 tacagaggct ttcgttgagt tggatcgcat catccctctg agagatggca ttgaccgggc
    13261 agacctgcag gcaaggggct tcctcacagt gatgacacac gacaggggca gtgagtgttg
    13321 acgtcttcgc cagggccagg cgcgggtgtt gctgtaaacc ttgcgtttta tgcacgtccg
    13381 aacaggcagc gagacaggta ttacatcctg tacaccacag tggttcggcc accacaaagc
    13441 ggttcatgtc ggctccttga aaatgatatg aaacattgca tttcatattg cataaaggat
    13501 gccattctga ttaactcaat atattcaata agttatgtat taactggatt ctgaacggta
    13561 caaaaatgat ggtcattaac gttcagcaaa aaatggattc gacacccgtg tcgcagggga
    13621 aagcgagaat gtgatcttga tcacgagaag tgaggtatgc tggccgcaag cgcagccagc
    13681 acggaatgga gcaaagtaat caggcgtgtt ctttcagcca gttcagcaca acgtcgtggt
    13741 gattgctggt tttgaaatcg tcaaagacat gttcgatttt gccatcagcg tcaatcagga
    13801 agctgatgcg atgaatgcca tcgtaggttt tgcccatgaa ggacttttca ccccagacgc
    13861 cgaattgttc gcacacctgg tggtcctcat cagacaggag cgtaaagtta agcagctctt
    13921 tttccgcaaa acgggagagt ttttcgggtt tatcggtgct gatacccagc acatcaacgc
    13981 ccgctttttt caactcatcc atgttatcgc gtaagccgca ggcctgtacg gtacagccgg
    14041 gggtcatggc tttcgggtag aaataaacca gaacacgctg tccctggaag tcggtcaaat
    14101 taacttgttc tccgtcttga tccggcaagc taaatttcgg tgcgatatca ccggctttca
    14161 gtggattcat tacttaactc catcctgttc atcatgttgg gaataattga cgacgttaat
    14221 actgccttgt gcattgagtt ctgtacatag ggctttgaac gcttgctcaa tatttgctgc
    14281 gtccgcagat gcggggctgt gggcggttat ctgaatatgc aactgcgcag cccgttcatt
    14341 ttcagcaggt tgcgtgcgcg acaccagctc cgcaatgttc atatgatgcg cgtcgaaaag
    14401 tgctgtgaag cgttcaatta aatgcgggga gtctgccaca tcgacctgaa cccagacaga
    14461 tgctggcatt ggcggacgcg gacgcgccgt cgtgcgcttc atcacgatta aaagatccag
    14521 ctcggcacct ttcaacggta acgttgattc aatcagagta atggcattcc atgaaccgga
    14581 aagcagcatg ataaacgtga actcttctcc cagcatcgcc aggcgactgt cttcaatatt
    14641 acagccgcaa ctactgacat gacgggtgat ggtgttcaca attccagggc gatcggcacc
    14701 caatgcagtg atcaccagat aatgttgcga tgacagtgtc aaactggtta ttcctttaag
    14761 gggtgagttg ttcttaagga aagcataaaa aaaacatgca tacaacaatc agaacggttc
    14821 tgtctgcttg cttttaatgc cataccaaac gtaccattga gacacttgtt tgcacagagg
    14881 atggcccatg ttcacgggaa gtattgtcgc gattgttact ccgatggatg aaaaaggtaa
    14941 tgtctgtcgg gctagcttga aaaaactgat tgattatcat gtcgccagcg gtacttcggc
    15001 gatcgtttct gttggcacca ctggcgagtc cgctacctta aatcatgacg aacatgctga
    15061 tgtggtgatg atgacgctgg agctggctga tgggcgcatt ccggtaattg ccgggaccgg
    15121 cgctaacgct actgcggaag ccattagcct gacgcagcgc ttcaatgaca gtggtatcgt
    15181 cggctgcctg acggtaaccc cttactacaa tcgtccgtcg caagaaggtt tgtatcagca
    15241 tttcaaagcc atcgctgagc atactgacct gccgcaaatt ctgtataatg tgccgtcccg
    15301 tactggctgc gatctgctcc cggaaacggt gggccgtctg gcgaaagtaa aaaatattat
    15361 cggaatcaaa gaggcaacag ggaacttaac gcgtgtaaac cagatcaaag agctggtttc
    15421 agatgatttt gttctgctga gcggcgatga tgcgagcgcg ctggacttca tgcaattagg
    15481 cggtcatggg gttatttccg ttacggctaa cgtcgcagcg cgtgatatgg cccagatgtg
    15541 caaactggca gcagaagggc attttgccga ggcacgcgtt attaatcagc gtctgatgcc
    15601 attacacaac aaactatttg tcgaacccaa tccaatcccg gtgaaatggg catgtaagga
    15661 actgggtctt gtggcgaccg atacgctgcg cctgccaatg acaccaatca ccgacagtgg
    15721 tcgtgagacg gtcagagcgg cgcttaagca tgccggtttg ctgtaaagtt tagggagatt
    15781 tgatggctta ctctgttcaa aagtcgcgcc tggcaaaggt tgcgggtgtt tcgcttgttt
    15841 tattactcgc tgcctgtagt tctgactcac gctataagcg tcaggtcagt ggtgatgaag
    15901 cctacctgga agcggcaccg cttgcggagc ttcatgcccc ggctggaatg attttgccgg
    15961 tgacctccgg tgattatgca atcccggtga ccaacggtag tggtgctgtc ggtaaggcgc
    16021 tggacattcg tccaccagcc cagccgctgg cactggtttc tggcgcgcgt acccagttca
    16081 cgggcgatac cgcttcattg ctggtggaaa atggtcgtgg caatactctg tggccgcagg
    16141 tggttagcgt gctgcaggcg aaaaactaca ccatcaccca acgtgatgat gctggtcaga
    16201 cactgaccac cgattgggta caatggaacc gtctggacga agacgagcag tatcgtggtc
    16261 gttatcaaat ctctgttaag ccgcagggtt atcagcaggc ggttacggtt aaactgttga
    16321 acctggaaca ggcgggcaaa ccggttgcag acgcggcttc catgcagcgt tacagcacgg
    16381 agatgatgaa cgttatttcc gccggtctgg ataaatctgc cactgacgcc gcgaacgctg
    16441 cgcaaaatcg tgcctccacc actatggacg tacaaagtgc agctgatgac accggtttac
    16501 caatgctggt cgtacgcggg ccgttcaatg tggtttggca acgtctgcca gcggcgctgg
    16561 aaaaagtggg catgaaagtg accgacagca cccgttcgca gggcaacatg gccgtaactt
    16621 ataagccgct gtctgacagc gactggcagg aactgggcgc gagcgatcca ggcctggcat
    16681 ccggtgacta taaactgcag gttggcgatt tagataaccg cagcagccta cagttcatcg
    16741 atccgaaagg tcatactctg actcagagtc agaacgacgc gctggtagct gtcttccagg
    16801 ctgcgtttag caagtaaaaa tacagggctg gaatcatccg gccctttttt ctgatatgat
    16861 acgcaaacgt gtgcgtcggc aggaaaacgc gattttagcg gtaattcgca cgaaatttgt
    16921 ttgtcggacg tagttcggat aaggcgttca cgccgcatcc gacaaaacat ccggcacacc
    16981 agacagcaaa agattttaaa acgttaattc acacccagga gtgataaaga tgcaaaagca
    17041 agctgagttg tatcgtggta aagcgaaaac cgtatacagc acggaaaacc cggacctgtt
    17101 ggtgctcgaa ttccgcaatg atacgtcagc aggggatggc gcgcgcattg agcagtttga
    17161 tcgcaaaggt atggtgaaca acaagttcaa ctacttcatt atgagcaaac tggctgaagc
    17221 gggtatcccg actcaaatgg agcgtctgct ctccgatacc gaatgtctgg tgaaaaagct
    17281 ggatatggtg ccggttgagt gtgtcgtgcg taaccgtgct gcgggctctc tggtgaaacg
    17341 tcttggaatc gaagaaggta ttgagctgaa cccgccgctg ttcgatctgt tcctgaaaaa
    17401 cgatgccatg cacgatccga tggtcaacga atcttactgc gaaacctttg gctgggtgag
    17461 caaagagaac ctggcgcgta tgaaagagct gacctacaaa gcgaacgacg tgctgaaaaa
    17521 actgttcgat gatgctggtc tgattctggt cgacttcaag ctggagtttg gtctgtacaa
    17581 aggtgaagtg gtactgggcg atgagttctc cccggacggc agccgcctgt gggacaaaga
    17641 aacactggag aaaatggaca aagaccgttt ccgccagagc ctcggtggcc tgatcgaagc
    17701 ctatgaagcc gtcgcccgcc gcctgggtgt acagctggac tgatttttct gttcatcatc
    17761 ttgccgtgca gctggcacgg caagacaacc gctctcggaa tatatgcatt gaacgctcat
    17821 tcctccgcca tttcatcccg ctttcctgtg gtaatcttcc tctgaaccac ctacgatcat
    17881 ttctataatg aatatattgt tgaggtatct atgcgttggc aagggcgacg tgaaagtgac
    17941 aatgtcgaag acaggcgtaa cagctccggc ggtccgtcaa tgggcggtcc cggttttcgc
    18001 ctgccaagcg gtaaaggcgg gctgatttta ctgatagtcg tgctggttgc aggctactat
    18061 ggtgttgatt taaccgggtt gatgaccgga cagccggttt cccaacagca atcaacacgg
    18121 tcaattagcc caaatgaaga cgaagccgca aaattcacct cggtgattct ggcaaccacg
    18181 gaagacacct ggggacagca attcgagaag atgggtaaaa cctatcagca accgaagctg
    18241 gtcatgtatc gtggaatgac gcgtaccggc tgcggggcgg gccagtccat tatgggaccg
    18301 ttctattgcc cggcggatgg cacggtttat atcgatctct ccttctatga tgacatgaaa
    18361 gacaaactcg gcgcggatgg cgattttgcc caggggtacg ttatcgccca tgaagtcggt
    18421 catcatgtgc agaaattgtt aggcatcgag ccgaaagttc gtcaactgca acaaaacgcg
    18481 acgcaggcgg aagtaaaccg cttatctgtg cgtatggaac tccaggccga ctgttttgcc
    18541 ggtgtctggg ggcataacat gcagcagcaa ggcgatctgg aagaggcgct gaacgcggcg
    18601 caggccatcg gcgatgaccg tttacaacag caaagtcagg ggcgagtagt accagacagt
    18661 ttcactcatg gtacttctca gcaacgctac agctggttta aacgtggttt cgacagcggc
    18721 gatccggcac aatgcaatac ttttggtaaa agcatttaac ttccggggca aggatggctg
    18781 aactgactgc gcttcacaca ttaacagcgc aaatgaaacg tgaagggatc cgccgcttgc
    18841 tggtgttgag cggggaagag ggttggtgtt ttgatcatgc gcttaagttg cgtgatgcct
    18901 tacctggcga ctggctgtgg atttcgccgc agccagatgc tgaaaaccac tgttctccct
    18961 cggcgctaca aactttactt gggcgcgagt tccggcatgc ggtattcgac gcccgccacg
    19021 gctttgatgc cgctgccttt gcggcactta gcggaacgtt gaaagcggga agctggctgg
    19081 ttttgttact ccctgtatgg gaagagtggg aaaaccaacc tgatgccgac tcgctgcgct
    19141 ggagtgattg ccctgaccct attgcgacgc cgcattttgt ccagcatctc aaacgcgtac
    19201 ttacggcgga taacgacgct attctctggc ggcaaaacca gccgttctcg ttggcgcatt
    19261 ttaccccccg tactgactgg caccccgcga ccggcgcacc acaaccagaa caacagcaac
    19321 tcttacagca gctactgacc atgccgccgg gcgtggcagc ggtaacggct gcgcgtgggc
    19381 gcggtaagtc cgcgctggca gggcaactca tttctcgtat tgcgggcagt gcgattgtca
    19441 ccgcgcccgc aaaagcggca acggatgtac tggcacaatt tgcgggcgag aagtttcgct
    19501 ttattgcgcc ggatgccttg ttagccagcg atgagcaagc cgactggctg gtggtcgatg
    19561 aagccgcagc catacctgcg ccgttgttgc atcaactggt atcgcgtttt cctcgaacgt
    19621 tgttaaccac tacggtgcag ggctacgaag gtaccggacg tggttttttg ctgaaatttt
    19681 gcgctcgctt tccgcattta caccgttttg aactgcaaca gccgatccgc tgggcacagg
    19741 gatgcccgct ggaaaaaatg gttagtgagg cactggtttt taacgatgaa aacttcaccc
    19801 atacaccaca aggcaatatc gtcatttccg catttgaaca aacgttatgg cgaagcgatc
    19861 cagaaacgcc gttaaaggtt tatcagttat tgtctggtgc gcactaccgg acctcgccgc
    19921 tggatttacg ccggatgatg gatgcaccag ggcaacattt tttacaggcg gctggcgaaa
    19981 acgagattgc cggggcgttg tggctggtgg atgagggcgg attatctcaa gaactcagtc
    20041 aggcggtatg ggcaggtttt cgtcgcccgc ggggtaatct ggtggcccag tcgctggcgg
    20101 cgcacggcag caatccactg gcggcgacat tgcgcggacg gcgggtcagc cggattgcag
    20161 tccatccggc gcgtcagcgc gaaggcgttg ggcaacagct cattgccagc gctttgcaat
    20221 ataggcctgg cctcgactat ctttcggtga gttttggtta caccggggag ttatggcgtt
    20281 tctggcaacg ctgcggtttt gtgctggtgc gaatgggtaa tcatcgtgaa gccagcagcg
    20341 gttgctatac ggcgatggca ctgttaccga tgagtgatgc gggtaaacag ctggctgaac
    20401 gtgagcatta ccgtttacgt cgcgatgcgc aagctctcgc gcagtggaat ggcgaaacgc
    20461 ttcctgttga tccactaaac aatgccgtcc tttctgacga cgactggctt gaactggccg
    20521 gttttgcttt cgctcatcgt ccgctattaa cgtcgttagg ttgcttattg cgtctgctac
    20581 aaaccagtga actggcatta ccggcgctgc gtgggcgttt acagaaaaac accagtgacg
    20641 cgcagttatg taccacactt aaactttcag gccgcaagat gttactggtt cgccagcggg
    20701 aagaggccgc gcaggcgctg ttcgcactta atgaggttcg cactgagcgt ctgcgcgatc
    20761 gcataacgca atggcaattt tttcactgac tccttcaatt ttctcccatg gtcattgtga
    20821 gtcagcggcg taaaattaat gacatcagtt aaggagttcg ccatgaaaca tgaccatttt
    20881 gttgttcaaa gcccggataa acctgcgcaa cagttgttac tgcttttcca cggtgtcggg
    20941 gataacccgg tggcaatggg ggaaataggc agctggtttg cgccgctatt tcctgatgcg
    21001 ctggtggtga gtgtcggagg cgcggagccg agcggtaatc cggcggggcg tcagtggttt
    21061 tcggtgcagg gtattacgga agataatcgc caggcgcgtg tggatgccat catgccgacg
    21121 tttattgaga tggtgcgcta ctggcagaaa cagagcggag taggggctaa cgccacagcg
    21181 ctaatcggtt tctctcaagg cgcgattatg gcgctggaga gcattaaagc cgaaccgggc
    21241 cttgcttcac gcgtcattgc ttttaacgga cgctatgcga gcctgccgga aactgcgtct
    21301 acagccacga caattcacct gattcatggc ggtgaagatc cggttatcga tctggcgcat
    21361 gcggttgcgg cgcaggaagc gttaatcagt gccggtggtg acgtgacgct ggatatcgtg
    21421 gaggatttag gccatgcgat cgacaatcgc agtatgcaat ttgccctgga tcacttgcgt
    21481 tataccattc cgaaacatta tttcgacgaa gcgctaagtg gcggtaagcc tggcgatgat
    21541 gatgtgattg aaatgatgta aaagacagat tacctggcct gataagacgc gtgagcgtcg
    21601 catcaggcat cgttcaccat tgccggatgc ggcgttaacg ccttatccgg cctacggttg
    21661 agcactcagc ttacttcttc ggttgatcct ttttcggcca gtcgtcgtca tcgtcccact
    21721 tatcgttgaa atcacgatgc gggggaagct ccggcttgtt ggcgagaaat ttcttgtggt
    21781 cgacgcgctt gagatcttta atcacgttca gcaggacgcc taccaaaaag acaatcacca
    21841 gaatccacca atatttagcc agccagtcca tgcttatttc ctcttgcaga accactcatc
    21901 aggcgacgag ctgttccatg atacgttgat acatacgggc aagtagctgc aggtcggcag
    21961 cgttcacaca ttcattaatt ttatgaatag tggcattgac cggcccgagt tccaccacct
    22021 gcgcccccat gcgagcaata aagcgcccgt cggacgttcc gcctgtggtc agtagctgcg
    22081 gtttaatttc attatagtgc tcaaccgcgt taacgaccgc atccaccagt ttaccgcgcg
    22141 cggtcaaaaa tggctgcccg gaaagccacc aatccaccgt atagcgcagt tgatgttttt
    22201 caagcagggc aagcacctgc gctttgatca tctcatcagt cagttcggtg ctgaagcgga
    22261 agttaaactg cacaaacagt tcacccggaa taacgttgtt actgcccgtt tccgcctgaa
    22321 tattggcaat ctgcatactg gtcgccggga agaattcatt gccctgatcc cactcaatag
    22381 ccactaattc attaaggaaa ggtgctgcgc gatgtactgg attgtcagcc agatgtgggt
    22441 aggccacatg cccctgcacg ccatgaatgg taaggttgca ggttaacgat ccgcgacgac
    22501 catttttcac cacatcacca accacttcga tactcgacgg ttcgccgacc agacagtaat
    22561 cgagacgctc attacgcgcc attaacgctt cgacgacttt taccgtaccg ttgtgggcac
    22621 tggcttcttc atcagaggtg atcagaaatg ccagtcgtcc cgtatggttg ggatgttgtg
    22681 cgacaaaacg ttctgctgct accaccatcg ccgccagcga gcctttcata tctgccgcac
    22741 cgcgcccgaa taacatgcca tcacgaatgg tgggttcaaa cggcggattg atccaacgat
    22801 cggcgtcgcc aggcggcacc acgtcggtat gcccggcaaa ggccaatgtt tcaccttgcc
    22861 cacgccatgc ccaaaaattc tgcgtatcgg caaagtccat gcgttcaacg gtaaaaccga
    22921 tcgcctgcaa acgatcaatc aacaaagcct ggcatcctgc gtcatcagga ctcagggaag
    22981 ggcggcgaat aagctgttgt gtcagctcaa taaccgggca cgacatagac tacacctcat
    23041 ggaaaaattg ctgataactg gaatcactga aacccagcag cataggctta ccgggcgcgc
    23101 agagcaatgg acgtttgata attgcaggca tttcagtcat taatgccgcc gctgaggccg
    23161 catcggtgat tttattgcgg gtggtttcgt ccagtttacg ccaggttgta ccacgggtgt
    23221 tgagtaacgc ctcccaacct aattcgttga taaaaccgtt caataattcg ctgtccagcc
    23281 catcgacgcg gtaatcatga aaacgatagt cgatgttatt ggcttccagc caacggcgag
    23341 cctttttaat ggtgtcacaa tttttgatac cgtaaagtgt aaccataatg aatccttttt
    23401 cgatttatga gaataaatac tcatttaagg gtgaaaacat tttgcctgca aatgattatc
    23461 aggaaatata acattatcga agtattcaaa ttaacaacta ttaaataata ttaaacattt
    23521 ttaacgtaaa cattaagaat attcatcttg ttatctgtct gtattttaac cagaattaaa
    23581 tcagtattag atgccgtatg aatatctcga aatgttgctg tgtatcacat acaaatcatc
    23641 accgcaggac catagtattt acataagatt tgtccccccg gaggtagtca tcgcagcaat
    23701 cgagtaaatt agcttcacca cggtatattg tttccgtaga atacccataa ttataagaga
    23761 ggttgttatg attgaacgtg aactggggaa ctggaaagac tttatcgaag ttatgcttcg
    23821 taagtaattc ctgagacaaa gtataaacag caagaacccg ctgagcaggt tcttaatcgg
    23881 aaagttgaaa aaggcgacac attggcatgt cgcctttttt attgcttatt ccgggcgcgg
    23941 ctttagcggg aagcggcggc gcaccagcac aaagaacagc ggcacgaagt aaatagccag
    24001 aatagtggct gaaatcatcc cgcccattac gccagtaccc accgcatgct gaccaccgga
    24061 accggcaccc gtgctggttg ccattggcaa tacgccgaag ataaatgcca gcgaggtcat
    24121 cagaatcggg cgtaaacgct gacggcaggc gtggagcgtc gcttcaaaca ggtcgtggcc
    24181 tttttggttc atctcgttag caaactcgac gatcaggatg gcgtttttcg ccgataaacc
    24241 aatgaccgtt aacaggccca cctggaagta aacgtcgttt tccagcccgc gcatccaggt
    24301 tgccagcagc gcgccgatta cccccagcgg cacgaccagc attaccgaga acggcaccga
    24361 ccagctttca tacaacgcag ccagacacag gaataccacc agcaaggaaa tggcgtacag
    24421 cgccggagcc tgcgcgccgg aaagtcgctc ctgatacgac atcgccgtcc actccagacc
    24481 aaagccgttt ggcagctgct tcactaacga ttccataata tccatcgccg taccggtact
    24541 gacccccggt gcggcttccc caacaatctc aaccgcagaa taaccgttat agcgttccag
    24601 acgcggcgag cctgtttccc agcgtgaggt cgcgaaagca gagaagggca ccatgccgcc
    24661 atctttattt cggacatacc agagattgat gtcatctggc agcatgcgat acggcgcagc
    24721 tgcctgcaca tagactttct tcacgcgacc gcgatccata aagtcattca catagctcga
    24781 accccaggcg gtttgcagtg tgtcgttaat atcgtcgata gcaacgccca gcgcctgagc
    24841 tttacgctgg tcgatatcaa tctgcaactg cggactgtcg tcgaggccgt tatggcgcac
    24901 acgggttagc tccgggtttt ccgccgccag cgccagcaac tggttacgtg ctgccatcag
    24961 cgcatcgtga cctgctccag cgtggtcctg caactccata tcaaaacccg cagaactacc
    25021 aagaccgcta attgctggcg ggctgctggc gataacgcga gcttctttaa tcttgttaaa
    25081 cgcctttgtt gcacgctcga taatggcaaa cgaggtgccg gtcttactgt cgcgttcgct
    25141 ccagtctttc aggcggataa acattcgcgc cacgttttgc ccgttacccc cagggccaga
    25201 accaacggtg gcaaacaccg acatgatgtt gtctttttca tgggtgaagt aatatttctc
    25261 gatttgctca acgactttca gggtctgttg ttgggttgaa ccgctgggca attgtaccga
    25321 ggtagtaaac atgccacggt cttccagcgg taagaacgac gtcggcaaac gcaggaacag
    25381 gaacaccatg ccgccaagca gcaggacata aatcacaatc cagcgcaggc tgcggtggag
    25441 aattttcgcc accccttttt cgtagcgttc ggcgttgcgg ttaaacatct ggttaaacca
    25501 ggcaaaaaag cctttttgcc cgtgatgctc accttttttc agtggcttaa gtagtgtggc
    25561 acacagagcc ggagtgagga tcatcgctac cagtactgac agcaccatcg ccgcaacaat
    25621 ggtaatagag aactggcgat agatggcacc ggtggtgccg ccgaagaagg ccattggtac
    25681 aaataccgcc gaaagcacca tcgcaatccc gaccagtgcc ccctggatct gccccatcga
    25741 tttacgtgtg gcttcgcgag gagtgagtcc ttcctcactc ataatacgtt cgacgttttc
    25801 caccaccacg attgcgtcat ccaccagcag accgatcgcc agtaccatcg cgaacatggt
    25861 taaggtgttg acgctgtaac cgaaggcgta aagtacggag aaggttccca tcaacaccac
    25921 cggcacggcg atagtgggta tcagcgtggc gcggaagttt tgcaggaaca gatacataac
    25981 gaggaaaacc agggcgatag cttccagcag cgttttcacc acgtcttcaa tggaggcttt
    26041 aacaaacgag gtggtttcat acgccacctt gtattccagt ccgtgcggga agtactgcgc
    26101 cagctcgtcg agacgattga gcaccagctc cgctgtcgcc atttcgtttg cgccggaggc
    26161 cagttttacc cccagcccgg aggctggctt accattgaag cggctaagat aatcgtattt
    26221 ctccgccccc atttcgacgg tggcgacatc gcccagcctt acctctgagc cgtcttgatt
    26281 cacccgcaag gtgatatcgc ggaactgttc tggtgtttgc agcagtgact gggcgttaat
    26341 ggtggcgttg agcgcctgct tatcgacgga aggtgtacca ccaagttgcc caaccgcaat
    26401 ctgcgcgttc tgtgactcaa tggcatcagt gacatcttta gccgtcatct ggaaactgtt
    26461 gagtttcgcc gggtccagcc agatacgcat ggaatattgc gaaccatagg catcgatatc
    26521 cccgacgcca ttcacgcggc ttaacgggtc ctgaatatta ctggcaacat aatcggcaat
    26581 atcctgttta tccatcgaac catcggtaga gacgaaagca atggtcagaa tattggtatc
    26641 gccggtttta cgcaccgtca cgccctgatt ttgcaccgcc tgcggtaact ttcgcatggc
    26701 tgattgcagc tggttttgta cttgctgcac ggcttcgtcc ggatcggtgc ctgctttaaa
    26761 acttaaagtg acagatgcct gaccgatgcc actgctctga gatgacatat acatgagatt
    26821 atcgaggccg gtcatatttt gctcgataac ctgggtcacg gtgttttcca gcgtctgggc
    26881 cgatgcgccg ggatagttag cggtcactcg cacattcggt ggcgcgagat cggggtattg
    26941 ttcaacgggc aatgaaaaaa tcgccagggt ccctgtcaga cacaacagga ttgccagcac
    27001 ccaggcaaaa atggggcgat caataaagaa attcgccatt aaaagaggac ctcgtgtttc
    27061 tgcgtatcgt tgtaggcttg acttgcttca ctgtagcggc aaggtacgag ccaatcgtgg
    27121 agaaaaaaag gagttaatgt aaattatcat gcccttttat actgaacctt aagtgcaagt
    27181 attctttggt cagtaggagg cattacatca actgactttc ctcaccctcc gcagagcgaa
    27241 agctaatact cactaacgtg ccgccgccgg aaggttgtga aaaagtcagc gtcccaccca
    27301 gccgttccgc gcgttcgcgc atgatattca gaccataatg accttcgggt tctttcggtt
    27361 caccgatacc aatcccgtta tcacggatat aaaccgtgtg attgccgtcc ggcgcggtga
    27421 cgcaactgac ggcgatttcg ctggcgttgg cgtgcttcat cgcattcagc accgcttcgc
    27481 gaataatttg caacaaatgc acctgcattt gcgcatccag cgccagggtt ggcagacggc
    27541 agtcgagggt cagtttggcg ctggtttgat tttgtaacgt atccagcatt tccctcaacg
    27601 cggaggggag atccgcctgc tgcagcgtca ggcggaaagt agtcaacagc tcgcgtaact
    27661 gccgataagc atcattcaac gcctgggaaa aatcggccat gatactttgt gcggtggcgt
    27721 tatcttccgg tatcgaacgc ttcagtaacg tcaactggat acgtaagtaa gaaagtacct
    27781 gagccagcga gtcgtgcaat tcgcgggcga tggttgcacg ttcttccatc aacaataact
    27841 gctgaaaatg cttctgcgcc tgattaaagt acaaaccgcg tcccagcatc gacgaaacgc
    27901 tgttaagcag cggttctgat gatgaaacgt gactattttg ccagtgcagt tcgccgtaaa
    27961 ccgtctcttg cattgtcacc ggtaaaatct gcatcggcaa ttccgggttt ggttgccctt
    28021 cgctaatccg ccagttttca ccgacattta actccagata ttcagccgct tcattgtcgc
    28081 gaacaatctg caaaatatgg cggaaacaat gcacatcaat ctggcttgtg ttcagcgcct
    28141 gcgaacattg atacaacacc tccagacgac gcttggcctc gtggagatcg cgggtctttt
    28201 cttctactga cgcctccagc gaaaggtaca atttatgcag ctcgctcgac atctggttaa
    28261 aggtttttgc aagcagacca agctcattcg gcagactggt atccagcggc ggcgagtcga
    28321 actgcccgtg ttcaatacgt tgactggcgg taaccagctg attcagcggg gcaaccacct
    28381 gatggcgtat acggcgcaaa gtaaaaaaga ccagcgtgaa aataccgata ccgccagcca
    28441 gggaaatcgc caccaccagc agcattttgc gttcagcgta gtgctgtaaa gccagcacga
    28501 acaggtctat ctgattaaca taattattaa tattggcctg ataccacggc aaatcgccct
    28561 tgctgagccg attattcatc tccagccagt tggcattcag atgcgcatag cgagttttta
    28621 ctgcttctgg cacataccag acgttgagat tggttaatac cgatgaatgc agtgcctgct
    28681 gaaacaactg gcgatgtgca ttgagttgtg gactgccact ttgcaagtcg tagcccaggc
    28741 ggtaactctg catacgcagc gatccggcaa tattgatagc ctcagcgtcg cgcaaactgc
    28801 tcgccagagt tagcagagcg atacccgtgg aaagaatcga cagcagcaca atgtaaaaaa
    28861 aggcccgggc cagactggcc gagacgggtc gtttaacaat cacacgcgtc ttctccacaa
    28921 aaaatcatta atgttccagt tcgctaaaac gcaatttagt cagtgatggg attatttgat
    28981 ctgccacagt ttatagcgaa atagttactt attctgggca catgaacatt gccgattccc
    29041 ctgtaagtcg ggtaataaca acactcatat aaagaataag gtttttacaa ccaaaaaaga
    29101 aggtcgttat gaatcgtttt attatggcca acagtcagca atgtctgggt tgtcatgctt
    29161 gtgaaatcgc ctgtgtcatg gctcacaatg atgagcaaca tgtcctgagc caacaccatt
    29221 ttcatccccg aattacggtt atcaaacatc agcagcaacg tagtgcagtg acctgtcacc
    29281 attgtgaaga tgcgccctgc gcccgtagct gccctaatgg cgcaatcagc cacgttgatg
    29341 acagcattca ggtcaatcag caaaagtgta ttggctgtaa atcctgcgtg gtggcctgtc
    29401 cgtttggtac gatgcaaatc gtcctgacac ccgtcgcggc aggaaaagta aaagccacgg
    29461 cgcataaatg cgacctttgt gcagggcgcg aaaacggtcc tgcctgtgtc gagaattgcc
    29521 cggcggacgc gctgcaactg gtcactgacg tcgcactctc cggcatggcg aaatcccgcc
    29581 gcttgcgcac cgcacgtcag gagcatcaac cgtggcatgc cagtaccgct gcacaagaaa
    29641 tgccggtaat gagtaaagtc gaacaaatgc aggcaacgcc cgcgcgtggc gagccggata
    29701 aactggcgat tgaagcgcgc aaaaccggtt ttgatgaaat ttatctgcca ttccgtgccg
    29761 atcaggcaca acgggaagcc tcgcgctgcc ttaaatgcgg cgagcacagc gtttgtgaat
    29821 ggacctgccc gctgcataac catattccgc agtggattga actggtgaaa gccggaaaca
    29881 tcgacgccgc cgtcgagctt tctcacctga ccaacaccct gccggaaatt accggacgcg
    29941 tttgtccgca ggaccgtttg tgtgaaggtg cctgtactat tcgcgatgag cacggcgcgg
    30001 taactatcgg caacattgaa cgctacattt cagatcaggc gttggcgaaa ggttggcgtc
    30061 ctgacttaag ccatgtcacc aaagtggaca agcgggtggc gattatcggt gcgggtccgg
    30121 cagggctggc ctgtgcggat gttctgaccc gcaatggcgt ggcggtgacg gtgtacgatc
    30181 gccatccaga aattggtggc ttgctcactt tcggcattcc ttctttcaaa ctggataaat
    30241 ccctgctggc acgccgtcgg gaaatcttca gcgcgatggg gattcacttc gaactcaatt
    30301 gtgaagtggg taaagatgtc tctttggatt cgcttttgga acaatacgac gcggtcttcg
    30361 ttggcgtagg cacttaccgt tccatgaaag cgggtttacc caatgaagat gcgccgggcg
    30421 tttatgacgc gctgccgttc ctcatcgcca acactaaaca ggtgatggga ctcgaagagc
    30481 taccggaaga gccgtttatc aataccgccg gactcaacgt cgtggtactg ggcggcggcg
    30541 acaccgcgat ggactgtgtg cgtaccgcgc tgcgccacgg cgcgagtaac gtcacctgcg
    30601 cttatcgtcg tgatgaagct aacatgccag gctcgaagaa agaggtgaag aacgcccgcg
    30661 aagagggggc caacttcgaa tttaacgtcc agccggtggc gcttgagctg aatgagcaag
    30721 gtcacgtttg cggaattcgt ttcctgcgca cgcgtcttgg cgagccggat gtccaggggc
    30781 gccggcgact tgtaccgatt gagggcagtg aatttgtcat gccagccgat gcggtgatta
    30841 tggcgtttgg cttcaatcca cacgggatgc cgtggctgga gtcgcacggt gtaacggtag
    30901 acaaatgggg ccgcatcatc gcggatgtgg aaagccagta ccgttaccag accaccaatc
    30961 cgaaaatctt cgccggtggc gacgccgtgc gtggtgcgga tctggtggtt accgcaatgg
    31021 cagaaggacg tcatgcggca caggggatta ttgactggct gggggtaaaa tcagtcaaat
    31081 ctcactgata gcctgcgcag acaaacccga cttcacagcg taatataatt gttcatttcg
    31141 cgctgtggag tcggtatgac gcaacaaatc accctcatta aagacaaaat tctctccgat
    31201 aactatttca ccctgcacaa cattacttac gatctcacgc gcaaagatgg cgaagttatc
    31261 cgccataaac gtgaagttta cgatcgcggt aatggtgcga cgatcctcct gtacaacgcg
    31321 aagaaaaaga ccgtggttct gattcgtcag ttccgtgtcg ctacctgggt taatggcaat
    31381 gaaagcgggc agctgattga aacctgcgcc gggctgctgg ataacgacga accggaagtg
    31441 tgcattcgta aagaagcgat tgaagagacg ggctatgaag tgggcgaagt gcgcaaatta
    31501 tttgaactgt atatgtcgcc tggcggtgtg actgagctaa tccacttttt tatcgccgaa
    31561 tacagtgaca atcagcgcgc taacgccggt ggcggtgttg aagatgaaga tattgaagtg
    31621 ctcgagctgc cgttcagcca ggcgctggag atgatcaaaa ccggcgagat acgtgacggt
    31681 aagacggtgt tattgcttaa ctatttgcaa acgtcacatt taatggactg aaaaataaca
    31741 ataatatttc gttgtttatt attggctcaa tccgacaaat ctgattgagc cgcgctacct
    31801 gcgcaacgaa gatacgactt gtgctgtttg tttgaacttc tggggtcgta ccgtccatgc
    31861 gctatcgcat tttccttctc ttttttttcg ctttgttgcc gacgtctttg gtgtgggcgg
    31921 cactagcgca acgggcgttt tccgactggc aggtcacctg taataaccaa aacttctgcg
    31981 tggcgcgcaa tacgggcgat cataatggac tggtgatgac cctgagccgc agcgccgggg
    32041 cgcataccga tgccgtttta cgtattgagc gcggcgggtt gaagtcgccg gatgcgtctg
    32101 aaggagagat agcgccacgg ctgctgttag atggtgagcc gttggcgtta agtggtgaca
    32161 agtggcggat ttcaccatgg ttattagtaa ccgatgatac ggcaaccatc accgcgtttt
    32221 tgcagatgat tcaggaaggg aaggcgatca ccttacgcga cggcaatcag accatttctc
    32281 tgagtggctt aaaagcggcg ttgttgttta ttgatgctca gcaaaagcgc gttggcagtg
    32341 aaaccgcgtg gatcaagaaa ggggacgaac cgccgctcag cgtaccgccc gcgcctgcgc
    32401 tgaaagaggt cgcggtggtt aacccaacgc cgacgccact ctcactcgaa gaacgcaacg
    32461 atttgctgga ttatggaaac tggcggatga atggtctgcg ctgctcgctt gatccgttac
    32521 gtcgtgaggt gaatgtcact gcgctgactg atgataaagc gctgatgatg atcagctgtg
    32581 aggcaggggc ctataacacc attgatttgg catggattgt gtcgcgtaaa aagccgctag
    32641 cctcgcgccc ggttcggttg cgtttgccgt tcaacaacgg gcaggagacg aatgaactgg
    32701 aactgatgaa cgcaacattt gatgagaaat cgcgtgaact ggtgacctta gcgaaagggc
    32761 gcggattaag cgattgtggc attcaggcgc gctggcgctt tgacggtcag cgttttcgcc
    32821 tggtgcgtta cgccgcagaa cccacctgcg ataactggca tgggccagac gcctggccca
    32881 cgttgtggat cacccgttaa acaaaatggc ggggacatgt aggccggata agacgctgta
    32941 ggccggataa gacgcgtcag cgtcgcatcc ggcaatcagc atccggcgat cactatcagg
    33001 cacctttcac tcccagcacc ttatgcgctt ttgccacaat attctcggcg gtaaagccaa
    33061 agaacgggaa cagcttatcc gccggagcag attccccata acccgtcatc ccgacaattg
    33121 cccctttcag accaacatac ttgtaccagt aatcggcaat acctgcttcc accgccacgc
    33181 gagccgcaac gttagaaggc aacaccgact cccgatattc ctcatcctgg gcgtcgaaaa
    33241 tatcggtcga gggcagggaa accacgcgaa cattgcgacc ttctcctgct aatttctccg
    33301 cagcttgcag ggtgatttcc atctctgaac cggtggcaat cagaataata tctggcttac
    33361 cgccgctgtc tttcagcaca taaccgccac gagtaatctc tttaacctga tccggcgtac
    33421 gttccacctg ggccagattc tgccttgaga ggatcagtgc cgtcggtccg ttgtggcgct
    33481 caaccgccag cttccagccc accgccgctt ccacctgatc gcacggtcgc caggtgctga
    33541 aatttggcgt taagcgcagg ctggccagtt gctcaacagc ctggtgcgtc ggaccatctt
    33601 cgcccaggcc aattgagtcg tgggtataaa ccataatctg ccgcgctttc atcagtgccg
    33661 ccatccgcgc ggcgttacgg gcgtattcaa caaacatcag gaacgtcgcg gtatacggca
    33721 caaagccgcc gtggtgcgcg atgccgttgg cgatagcggt catgccaaat tcacgcaccc
    33781 cgtagtgaat gtagttgccc gccggatctt ccttcagcga aacagaacct ttccagatgg
    33841 tcaggttgct gggagccaga tccgccgaac cgccgagcag ctccggtagc aaggggccat
    33901 aagcgttaag cgtattttgc gaagccttac gggtggcgat tttcgccgga ttcgcctgca
    33961 actcattgat atacttctga gtcgttttct cccagtcctt cggtaaacca ccgctcatcc
    34021 gacgggtaaa ctcttctgcc agttgcggat gagccttttt ataggcggca aacttctcat
    34081 tccacctctg ctgcgctttt tcgccttttt cgcgggcatc ccaggcgtga taaatatctt
    34141 tagggatctc aaatggcgga tggtgccagc ccagtttttg ccgtgccagc gccacttctt
    34201 cttcccccag cggtgcgccg tgcgcctctt ccttacctgc tttattcggc gatccaaagc
    34261 caatcaccgt acgacagata atcagcgacg gcttatcttt cacgctttgc gcttcaagga
    34321 tcgcttcctt caccgcctgc ggatcgtgac cgtcgatttc atggatcaca tgccagtgat
    34381 aggcttcaaa acgttttgcc gtatcgtcgg taaaccagcc ttctgtttca ccgtcgatgg
    34441 aaataccgtt gtgatcgtaa aaaccaatca gcttgcccag tcccagcgtg cctgccagcg
    34501 aacagacttc gtgggaaata ccttccatca ggcagccgtc gcccataaac acataggtga
    34561 agtgatcgac gatctcatgg tctggctggt taaactgcgc cgccagtgta cgctctgcta
    34621 tcgccagccc gacggcgttc gccaaacctt gtccaagtgg accggtggtg gtttcaacgc
    34681 ctggcgtata accaatctcc gggtggcctg gggttttcga atgcagttga cgaaagttct
    34741 tcagctcttc cagcggcagg tcgtaaccgg tcagatgcag caaactgtag agcagcatcg
    34801 acgcgtgacc gttggaaaga ataaagcggt cgcgatcata ccaggttggg tcggtagggt
    34861 tatgtttaag aaaatcgttc cacagcactt cggcaatatc agccatgccc atcggcgcgc
    34921 cgggatgacc agaattggct ttttgtaccg catccatact gagtgcgcga atcgcattgg
    34981 caaggtcttt tcgggacata taacactccg tggctggttt atagtttggc ggcaagaaga
    35041 tcttccagtt tgcgttgatc aacggcgaac agacgaatgc cttcagacag tttttctacc
    35101 gccatcgcat actgattgtg ctcccaacgg aactccgctt cgctcatggg agctgggcgt
    35161 gggaacgtct gagaaggtgg gattaattta cgtaccactg gcgaaacttt ttcctgcagc
    35221 tccttcagca aattcggtgc gatagtcagt cgatcgcagc cggttaaggc gaggatttgt
    35281 tcggtgcgac ggaagctcgc gcccatcaca atggtttcat agtggtgttg cttatagtag
    35341 tcgtagatat tgcgcaccga tttaacgccc ggatcttctt ccaccacata cgggtccatc
    35401 ggcttgcgtg cctgatacca gtcataaata cgcccgacaa acggcgaaat cagaaaaacg
    35461 cctgcttccg cacaggcccg tgcctgtgca aaagaaaaca gcagcgtcag gttgcagtta
    35521 ataccttctt tttccagctc ttctgccgcg cgaattcctt cccaggtcga agccagcttg
    35581 atcagaatgc gtgatttctc aacgccttgt tgctgataca agtccaccag atggcgcgcc
    35641 ttctcaatac tcttttcttt atcaaaagag aggcgtgcat caacttctgt tgacacgcga
    35701 ccgggtacga ttttgaggat ttcagcaccg aaattgaccg ccagtttgtc acacgctgcg
    35761 accacctgtt gttcctgggt cttgccattt tttttacccc aggcgatagc atcgtctatt
    35821 aaatgctcat attgtgataa tccggcagcc ttgagtaaca gcgaaggatt ggtggtggca
    35881 tcctggggat gataatggcg aatggactca atatcgccgc tgtctgccac gacagtggtg
    35941 aactgtttga tgccgtctaa ctcgttcata ggaaatactc cttgaaaagt aaagtgttag
    36001 atgagtgcgt taattcacac ttctgagaaa tttcgctaaa cgcatcaaaa aagcatagca
    36061 gacaggcatg gtattgctgg attaagcagg taacatcagt gttataggat tattaccaaa
    36121 acattatatt aatggtgaga gtttggactt gctcaaagtc tgtagactcc ggcaaggtaa
    36181 taatgtgcgc cacgttgtgg gcagggggtg ttgcccacac actttatttg tgaacgttac
    36241 gtgaaaggaa caaccaaatg gatgaccagt taaaacaaag tgcacttgat ttccatgaat
    36301 ttccagttcc agggaaaatc caggtttctc caaccaagcc tctggcaacg cagcgcgatc
    36361 tggcgctggc ctactcaccc ggcgttgccg caccttgtct tgaaatcgaa aaagacccgt
    36421 taaaagccta caaatatacc gcccgcggta acctggtggc ggtgatctct aacggtacgg
    36481 cggtgctggg gttaggcaac attggcgcgc tggctggcaa accggtgatg gaaggcaagg
    36541 gcgttctgtt taagaaattc gccgggattg atgtatttga cattgaagtt gatgaactcg
    36601 acccggacaa atttattgaa gttgtcgctg cgctcgaacc gaccttcggc ggaatcaacc
    36661 tcgaagacat taaagcgcca gaatgtttct atattgaaca gaaactgcgc gagcggatga
    36721 atattccggt attccacgac gatcagcacg gcacggcaat catcagcact gccgccatcc
    36781 tcaacggctt gcgcgtggtg gagaaaaaca tctccgacgt gcggatggtg gtttccggcg
    36841 cgggtgccgc agcaatcgcc tgtatgaatc tgctggtagc gctgggtctg caaaaacata
    36901 acatcgtggt ttgcgattca aaaggcgtta tctatcaagg ccgtgagcca aacatggcgg
    36961 aaaccaaagc cgcatatgcg gtggtggatg acggcaaacg taccctcgat gatgtgattg
    37021 aaggtgcgga tattttcctg ggctgttccg gcccgaaagt gctgacccag gaaatggtga
    37081 agaaaatggc tcgggcacca atgattctgg cgctggcgaa tccggaaccg gaaattctgc
    37141 cgccgctggc gaaagaagtg cgtccggatg ccatcatctg caccggtcgt tctgactatc
    37201 caaaccaggt caacaacgtc ctttgcttcc cgttcatctt ccgtggcgcg ctggacgttg
    37261 gcgcaaccgc catcaacgaa gagatgaaac tggcggcggt acgcgcgatt gcagaactgg
    37321 cccatgcgga acagagcgaa gtggtggctt cagcgtatgg cgatcaggat ctgagctttg
    37381 gtccggaata catcattcca aaaccgtttg atccgcgctt gatcgttaag atcgctcctg
    37441 cggtcgcgaa agcggcgatg gagtcgggcg tggcgacgcg tccgattgct gatttcgacg
    37501 tctacatcga caagctgact gagttcgttt acaaaaccaa cctgtttatg aagccgattt
    37561 tctcccaggc tcgcaaagcg ccgaagcgcg tagttctgcc ggaaggggaa gaggcgcgcg
    37621 ttctgcatgc cactcaggaa ctggtaacgc tgggactggc gaaaccgatc cttatcggtc
    37681 gtccgaacgt gatcgaaatg cgcattcaga aactgggctt gcagatcaaa gcgggcgttg
    37741 attttgagat cgtcaataac gaatccgatc cgcgctttaa agagtactgg accgaatact
    37801 tccagatcat gaagcgtcgc ggcgtcactc aggaacaggc gcagcgggcg ctgatcagta
    37861 acccgacagt gatcggcgcg atcatggttc agcgtggcga agccgatgca atgatttgcg
    37921 gtacggtggg tgattatcat gaacatttta gcgtggtgaa aaatgtcttt ggttatcgcg
    37981 atggcgttca caccgcaggt gcaatgaacg cgctgctgct gccgagtggt aacaccttta
    38041 ttgccgatac ctacgtcaat gacgaaccag acgcagaaga gctggcggag atcaccttga
    38101 tggcggcaga aactgtccgt cgtttcggta ttgaaccgcg cgttgctctg ttgtcgcact
    38161 ccaactttgg ttcttctgac tgcccgtcgt cgagcaaaat gcgtcaggcg ctggaactgg
    38221 tcagggaacg tgcgccagaa ctgatgattg atggtgaaat gcacggcgat gcggcgctgg
    38281 tggaagcgat tcgcaacgac cgtatgccgg acagcccttt gaaaggttct gccaatattc
    38341 tggtgatgcc gaacatggaa gctgcccgca ttagttacaa cttactgcgc gtttccagct
    38401 cggaaggtgt gactgtcggt ccggtgctga tgggcgtggc gaaaccggtt cacgtgttaa
    38461 cgccgatcgc atcggtgcgt cgtatcgtca acatggtggc gctggccgtg gttgaagcgc
    38521 aaacccaacc gctgtaattc ttttttaact ctcacgctta ccctgaatat tcagggtaag
    38581 cggtttggct gcaatatatt agtaaagctt attactgagt ttgcgaataa taaaaaaaag
    38641 cagtctatat aatatctcga tattatttta tttatattca tgcgttgcat atgaaagttt
    38701 atgcaccaca gcgaatatct ctccattctt agtgatctac ctcacctttt aaacgcgctt
    38761 gccgaatttt gttatttact ctgacgaaaa attgtcacga tacacgaaag tttttcacag
    38821 gcggcgactc atggataaag aacgcatcat tcaggaattt gtgcccggca aacaggtcac
    38881 actggcgcat ctcatcgcgc atcccggtga ggaactggcg aagaaaatcg gcgttccgga
    38941 tgcgggcgca attggcatta tgacgctaac tcccggcgaa acggcgatga tcgtcggtga
    39001 tttagcgctg aaagccgctg atgtgcatat cggttttctc gaccgcttca gcggtgcatt
    39061 ggtgatttat ggctcggtgg gcgcagtaga agaagcgtta tcacaaaccg tcagcggttt
    39121 aggtcgctta ttaaattata cgctttgtga aatgaccaaa agttaatacg aggcattcat
    39181 gaaacgaatt gcgtttgtgg gtagcgtcgg ggcaggaaaa acaacgttat ttaatgcgtt
    39241 gcaggggaat tataccctcg ccagaaaaac acaggccgtg gaatttaatg ataagggcga
    39301 tattgatacg ccgggtgaat attttagcca tccccgctgg tatcacgcct taattaccac
    39361 gctacaggat gtagacatgc tgatctatgt ccatggcgca aatgacccgg aaagtcggtt
    39421 gcctgccggg ttgctggata ttggcgtcag caaacggcaa atcgccgtca tcagtaaaac
    39481 ggacatgcca gatgctgacg tcgccgcaac gcgaaagtta ttgcttgaga cgggtttcga
    39541 agagccgatt ttcgagctaa acagccacga cccgcaaagc gtgcagcaac tggtggatta
    39601 tctggcatct ctcaccaaac aggaggaagc aggtgaaaaa actcatcaca gcgaatgata
    39661 ttcgtgaagc acacgcgcgc ggcgaacagg caatgtccgt ggttctgcgc gccagcatta
    39721 ttaccccgga agcccgtgaa gtggcggatc tactgggttt taccatcacc gaatgtgatg
    39781 aatcgatccc ggtaacggcg tccgtgcccg ccagcgtgcc agccgacaaa accgaaagcc
    39841 agcgcatccg cgaaaccatc attgcccagc tgccggaagg ccagtttacc gaaagcctgg
    39901 tcgcacagct gatggaaaaa gtgatgaagg aaaaacagtc gctggagcag ggcgcaatgc
    39961 agccgagctt caaatcggtg accggcaaag gcggcatcaa agtgattgat ggcagcagcg
    40021 tcaagtttgg tcgttttgat ggcgcacaac cgcattgcgt gggcttgact gatcttgtga
    40081 ccggagacga cggcagcagc atggccgccg gttttatgca gtgggagaac gcattcttcc
    40141 cgtggaccct gaactacgac gaaatcgaca tggtgctgga aggggagctc catgttcgcc
    40201 acgaaggcga aaccatgatc gccaaagcag gcgacgtgat gtttatcccg aaaggctcca
    40261 gcatcgaatt tggtacgaca tccagcgtga aattcctgta tgtcgcctgg ccggctaact
    40321 ggcaatccct atgaaagatt tcatcaccga agcatggcta agagcgaacc atacgctcag
    40381 cgaaggcgca gagatccatc tccctgcgga cagtcgcctg acgccctctg cccgggagtt
    40441 gctggaaagc cgccatctgc gcatcaagtt tattgacgag cagggccgcc tgtttgttga
    40501 cgatgaacag cagcagccgc agcccgttca tgggctgacc agtagcgatg aacatccgca
    40561 ggcgtgctgc gaactgtgcc gccagccggt ggcaaaaaag ccggatacgc tgactcacct
    40621 gtcggcggaa aaaatggtcg ccaaaagcga tccgcgtctg ggttttcgcg cggttctcga
    40681 cagcaccatt gcgctggcag tgtggctgca aattgaactg gcggaaccgt ggcagccgtg
    40741 gctggcggat atccgttcgc gtcttggcaa cattatgcgc gccgatgcgc tgggggaacc
    40801 gctgggctgc caggcgattg tcgggctttc tgacgaagat ctgcatcgac tttctcacca
    40861 gccgctgcgc tatctcgacc acgatcatct ggtgccggaa gccagccacg ggcgcgatgc
    40921 ggcgttgctc aatctgctac gtaccaaagt gcgggaaacc gaaaccgtag cggcgcaggt
    40981 gtttatcacc cgcagctttg aagtattacg cccggacatc ttgcaggcgc tgaaccgcct
    41041 ttcgagcacg gtctacgtaa tgatgatcct gagcgtaacg aagccgccgc taacggtgaa
    41101 gcagatccaa caacgactgg gggaaacgca atgattattg aacgttgtcg tgaactggcg
    41161 ttgcgagcgc ccgccagagt ggtttttccg gatgcgttag atcaacgtgt gctgaaagct
    41221 gcgcaatatt tacatcaaca aggtctggca acgcccattc tggtcgctaa tccgtttgaa
    41281 cttcgtcagt ttgcgctcag tcacggcgtg gcgatggacg ggctacaggt gattgatccg
    41341 catggcaacc tcgcaatgcg ggaagaattt gctcatcgct ggctggcccg cgcgggcgaa
    41401 aaaacgccgc cggatgcgct ggaaaaactt accgatccgc tgatgttcgc cgcagcaatg
    41461 gtcagcgccg gtaaagcgga tgtctgtatc gcgggcaacc tctcttccac ggcgaatgtg
    41521 ctgcgtgccg gattacgtat tatcggcttg cagccaggct gtaaaacgct ctcatccatt
    41581 ttcctgatgc tgccacagta cagcggtccg gcgttgggct ttgccgattg cagcgtggtg
    41641 ccacagccga cggcggcgca actggcggat atcgcgcttg ccagtgccga aacctggcgc
    41701 gccctcaccg gagaagagcc gcgcgtggcg atgctgtcgt tttccagtaa cggtagcgcc
    41761 cgtcacccct gcgttgccaa cgtccagcag gcgacagaaa tcgtccgtga gcgcgcacca
    41821 aagctggtgg ttgatggcga gttgcagttt gacgccgcct tcgtgccgga agtggcggcg
    41881 caaaaagcgc ctgccagccc gctacagggc aaagccaatg tgatggtttt tccgtcgctg
    41941 gaagccggaa atattggcta caaaatcgca caacgactcg gcggatatcg tgccgtcggg
    42001 ccattgatac aaggacttgc cgcgccgatg cacgatctct ctcgtggttg tagcgtgcag
    42061 gaaattatcg agctggcgct ggtggcagct gtgccgcgtc agacagaagt gaaccgcgaa
    42121 agcagtttac aaacactggt tgaatgaacg gtcccgttct ggaccctgat aaagaggaaa
    42181 gcacaatgga agcattagga atgatcgaaa cccggggcct ggttgcactg attgaggctt
    42241 ctgacgcgat ggttaaagct gcgcgcgtca agctggttgg tgttaagcag attggcggcg
    42301 gcctgtgtac tgccatggtt cgtggtgatg ttgcagcctg taaagccgcg accgatgcag
    42361 gtgccgcagc ggcacagcgt atcggcgagc tggtatctgt acacgttatc ccgcgtccgc
    42421 acggcgacct ggaagaagtc ttcccgatcg gcctgaaagg cgatagcagc aacctgtaat
    42481 caccctgtgt gacgtcgatt cgggcgtcac gctctctcct ttttcatttt accttctgcg
    42541 gagggtaggg ggagctttta cctgaaatac gccacggagg cgggtatgaa actggcagtc
    42601 gtcactggac aaattgtttg taccgtacgc catcacggac tggcgcatga caaattgctg
    42661 atggtggaaa tgattgatcc acaaggtaat cctgacgggc aatgcgccgt cgccatcgac
    42721 aatattggcg cgggaaccgg ggagtgggtg ttactggtga gtggcagttc cgcccgccag
    42781 gcgcataaaa gcgaaacgtc accggtcgat ctgtgcgtga ttggcattgt cgatgaggtg
    42841 gtgtctggcg gtcaggtaat tttccacaaa taaggcagaa catcatgaat caacaggata
    42901 ttgaacaggt ggtgaaagcg gtactgctga aaatgcaaag cagtgacacg ccgcccgccg
    42961 ccgttcatga gatgggcgtt ttcgcgtccc tggatgacgc cgttgcggca gccaaagtcg
    43021 cccagcaagg gttaaaaagc gtggcaatgc gccagttagc cattgctgcc attcgtgagg
    43081 caggcgaaaa acacgccaga gatttagcgg aacttgccgt cagtgaaacc ggcatgggcc
    43141 gcgttgaaga taaatttgcc aaaaacgtcg ctcaggcgcg cggcacgcca ggcgttgagt
    43201 gtctctctcc gcaggtgctg accggcgaca acggcctgac tttaattgaa aacgcgccct
    43261 ggggcgtggt ggcttcggta actccgtcca ctaacccggc ggcaaccgta attaataacg
    43321 ccatcagcct gattgccgcg ggcaacagcg tcatttttgc cccgcatccg gcggcgaaaa
    43381 aagtctccca gcgggcgatc acgctgctca accaggcgat tgttgccgca ggcgggccgg
    43441 aaaacttact ggttactgtg gcaaatccgg atatcgaaac cgcgcagcgt ttgttcaagt
    43501 ttccgggcat cggccttctg gtggtgaccg gcggcgaagc ggtagtagaa gcggcgcgta
    43561 aacacaccaa taaacgtctg attgccgcag gcgctggcaa cccgccggta gtggtggatg
    43621 aaaccgccga cctcgcccgt gccgctcagt ccatcgtcaa aggcgcttct ttcgataaca
    43681 acatcatttg tgccgacgaa aaggtactga ttgttgttga tagcgtagcc gatgaactga
    43741 tgcgtctgat ggaaggccag cacgcggtga aactgaccgc agaacaggcg cagcagctgc
    43801 aaccggtgtt gctgaaaaat atcgacgagc gcggaaaagg caccgtcagc cgtgactggg
    43861 ttggtcgcga tgcggcgaaa atcgcggcgg caatcggcct gaacgtgccg caagaaacgc
    43921 gtttgctgtt tgtggaaacc actgcagaac atccgtttgc cgtgaccgaa ctgatgatgc
    43981 cggtgctgcc cgtcgtgcgc gtcgccaacg tggcggatgc cattgcgctg gcggtgaaac
    44041 tggaaggcgg ttgccaccac acggcggcaa tgcactcgcg caacatcgaa aacatgaacc
    44101 agatggcgaa tgctattgat accagcattt tcgttaagaa cggaccgcgc attgccgggc
    44161 tggggctggg cggggaaggc tggaccacca tgaccatcac cacgccaacc ggtgaagggg
    44221 taactagcgt gcgtacgttt gtccgtctgc gtcgctgtgt attagtcgat gcgtttcgca
    44281 ttgtttaagg agtaacagat ggcgcacgac gaacaatggc tcaccccacg tctgcaaacg
    44341 gcggcaacgc tgtgtaacca gacgcccgcc gcgacggaat ctccgctgtg gctgggggtg
    44401 gatctgggca cctgcgatgt ggtgtcgatg gttgtcgacc gcgacggtca gtcggtggcg
    44461 gtatgcctcg actgggccga tgtggtccgc gacggcatcg tctgggattt cttcggcgct
    44521 gtcaccattg ttcgtcgcca tctcgacacg ctcgagcagc aattcggtcg ccgttttagc
    44581 catgcggcga cttcatttcc tccaggtacg gacccgcgta tttccatcaa cgtgctggag
    44641 tctgctggac tggaagtgag ccatgtgctg gatgagccaa ccgcagttgc ggatctgctg
    44701 caactggaca acgccggtgt agtggatatc ggcggcggca ctaccggcat tgccatcgtg
    44761 aaaaagggca aggtgacgta ctcggcggat gaagcaaccg gcggacatca catctctctg
    44821 actctcgccg gaaatcgccg tatttcgctg gaagaggcgg agcagtacaa gcgcggtcac
    44881 ggtgaagaga tttggcctac ggtgaaaccg gtgtacgaaa aaatggcgga cattgtcgcc
    44941 cggcatattg aagggcaggg cattactgat ttatggctgg cgggcggctc ttgtatgcaa
    45001 ccgggcgtgg cggaactgtt tcgcaaacaa ttcccggcgt tacaggtgca tttaccgcag
    45061 cacagtttgt ttatgacccc gctggcgatc gccagtagtg ggcgagagaa agcggagggg
    45121 ctatatgcaa aatgaattgc agaccgcgct ctttcaggcg ttcgataccc tgaatctgca
    45181 acgggtaaaa acatttagcg ttccaccggt gacgctttgc ggtccgggct cggtgagcag
    45241 ttgcggacag caagcgcaaa cgcgtgggct gaaacatctg ttcgtgatgg cagacagctt
    45301 tttgcatcag gcagggatga ccgccgggct gacgcgcagc ctggctgtta aaggcatcgc
    45361 catgacgctc tggccatgtc cggtgggcga accgtgcatc accgacgtgt gtgcagccgt
    45421 ggcgcagttg cgtgagtcag gctgtgatgg ggtgatcgca tttggcggcg gctcggtgct
    45481 ggatgcggcg aaagccgtgg cgttgctggt gacgaacccg gatagcacgc tggcagagat
    45541 gtcagaaacc agcgttctgc aaccgcgctt gccgctgatt gccattccaa cgaccgccgg
    45601 aaccggctct gaaaccacca atgtaacggt gattatcgac gcggtgagcg ggcgcaagca
    45661 ggtgttagcc catgcctcgc tgatgccgga tgtggcgatt ctcgacgccg cgttgaccga
    45721 aggtgtgccg tcgcatgtca cggcgatgac cggcattgat gcgttaaccc atgccattga
    45781 agcatacagc gccctgaacg ctacaccgtt taccgacagt ctggcgattg gtgccattgc
    45841 gatgattggc aaatcgctgc cgaaagcggt gggctacggt cacgaccttg ccgcgcgcga
    45901 gagcatgttg ctggcttcat gtatggcggg aatggcgttt tccagtgcgg gtcttgggtt
    45961 gtgccacgcg atggcgcatc agccgggcgc ggcgctgcat attccacacg gtctggcgaa
    46021 cgccatgttg ctgccaacgg tgatggaatt taaccggatg gtttgtcgtg aacgctttag
    46081 tcagattggt cgggcactgc gaactaaaaa atccgacgat cgtgacgcta ttaacgcggt
    46141 aagtgagctg attgcggaag ttgggattgg taaacgactg ggcgatgttg gtgcgacatc
    46201 tgcgcattac ggcgcatggg cgcaggccgc gctggaagat atttgtctgc gcagtaaccc
    46261 gcgtaccgcc agcctggaac agattgtcgg actgtacgcg gcggcgcaat aaatgccgga
    46321 tgcgacgctt gccgcgtctt atccagccta cgggattgca catgtagggc ggataaggcg
    46381 ttcacgccgc atccgccagt ggcgcggtgc aattgccgga tgcgatgctt gccgcgtctt
    46441 atccggccta cgggattgca catgtagggc ggataaggcg ttcacgccgc atccgccagt
    46501 ggcgcggtgc aattgccgga tgcgacgctt gccgcgtctg atccggcctt cgggattgca
    46561 catgtagggc ggataaggcg tttacgccgc atccgccaat aaataacaac taacagggag
    46621 taaaggcgat gggaattaac gaaatcatca tgtacatcat gatgttcttt atgctgatag
    46681 ctgccgtaga caggatcctg tcgcagttcg gcggttctgc tcgtttcctc ggtaagttcg
    46741 gtaaaagtat cgaaggatca ggcggtcagt tcgaagaagg ctttatggca atgggcgctc
    46801 tgggtctggc gatggtcggt atgaccgcgc tggcaccggt actggctcac gtactcgggc
    46861 cggtaattat cccggtttac gaaatgctcg gcgcaaaccc gtcaatgttc gccggaacgc
    46921 tgctggcgtg cgatatgggc ggcttcttcc tcgccaaaga gctggcgggc ggcgacgtag
    46981 cagcgtggct atactctggg ttaattcttg ggtcgatgat ggggccaacg attgtgtttt
    47041 ccattccggt ggcgctcggc attatcgaac cttctgaccg tcgttatctg gcgctcggcg
    47101 tgctggcggg cattgtgacc attccgattg gctgtattgc tggtggtctg gttgctatgt
    47161 actccggtgt gcagatcaac ggtcagccgg tggaattcac tttcgccctg atcctgatga
    47221 acatgatccc ggtgatcatt gttgcgattc tggtggcgct ggggctgaaa ttcatcccgg
    47281 aaaaaatgat caacggcttc cagatcttcg ccaaattcct cgttgcattg atcaccctcg
    47341 gtcttgctgc cgcggtagtt aagtttctgc ttggctggga actgatcccc ggtctggacc
    47401 ctatctttat ggcccctggc gataaacccg gtgaagtgat gcgcgccatt gaagttatcg
    47461 gttctatctc ctgcgttctg ttaggggcgt atccaatggt gctgctgctg acccgctggt
    47521 ttgaaaaacc gctgatgagc gtcggtaaag tgctgaatat gaacaacatc gcggcagccg
    47581 gcatggtggc aacgcttgcc aacaacatcc cgatgttcgg catgatgaag cagatggata
    47641 cccgcggcaa agtcatcaac tgcgccttcg ccgtttccgc tgctttcgcc ctgggcgacc
    47701 acttaggctt cgccgctgcc aacatgaacg ccatgatctt cccgatgatt gtcggcaagt
    47761 tgatcggcgg cgtaacggcg attggcgtgg cgatgatgct ggtgccaaaa gaagacgcga
    47821 ccacggctaa aaccgaagcg gaggcacaat cgtgaacact cgccagctat tgagcgtcgg
    47881 tatcgatatc ggcaccacca ccacccaggt gattttctcc cacctggagc tggttaaccg
    47941 tgcggcggtg tcgcaggtgc cgcgctacga attcattaaa cgcgaaatta gctggcaaag
    48001 cccggtgttc tttacccctg tcgataaaca gggtggttta aaagaagcgg aactgaaaac
    48061 cttaatcctc gagcaatatc aggctgcggg cattgcgccg gaaagcgttg attctggtgc
    48121 catcatcatc accggtgaaa gcgcgaaaac ccgcaatgct cgtccggcgg tgatggtgct
    48181 ctcccagtcg ctgggcgatt ttgtcgttgc cagcgccggg ccgcacctcg aatccgtcat
    48241 tgccggtcac ggtgccgggg cacaaaccct ttctgaacaa cggctgtgtc gggtactgaa
    48301 tattgacatc ggcggtggca ctgcgaacta cgccctgttc gatgccggaa aaatcagcgg
    48361 cactgcctgc ctcaacgtcg gtggtcgcct gctggaaacc gacagccagg ggcgcgtggt
    48421 ttacgctcat aaaccggggc agatgattgt ggatgagtgc ttcggcgcag gcaccgacgc
    48481 ccgttcgctg accggcgcgc agctggtgca ggttacccgg cggatggcag aactgattgt
    48541 cgaagtgatt gacggaacgc tctcgccgct cgcgcaggca ttgatgcaaa ccggtttgct
    48601 gcccgcaggt gttacgcccg aaatcattac gctttctggc ggcgtgggcg aatgttatcg
    48661 ccaccagccc gccgacccgt tctgttttgc cgatattggc ccgctgctgg caacggcgct
    48721 gcatgaccat ccgcgcctgc gtgagatgaa cgtacagttt ccggcgcaaa ccgtgcgcgc
    48781 cacggtgatt ggcgcgggcg cgcataccct ttcgctctct ggcagcacaa tctggcttga
    48841 gggcgtacaa ctgccgctgc gcaatttgcc ggtggcgatc ccgattgatg aaacggatct
    48901 ggtgagtgcc tggcaacagg cgctgcttca gctggatctt gatcccaaaa ctgacgcgta
    48961 cgtgctggcg cttcccgcct cgctgcctgt gcgttacgcc gcggtactga cggtcatcaa
    49021 cgcgctggtc gatttcgtcg cgcgttttcc gaatccgcat cccctgctgg tggtggccgg
    49081 gcaggacttt ggtaaagctc tgggcatgtt gttgcgccca cagctacaac aactcccgtt
    49141 ggcagtcatt gacgaagtga ttgtccgcgc gggggactat atcgacattg gtacgcctct
    49201 ttttggcgga tcggttgtgc cggtgacggt gaaatcactc gcatttcctt cctgagggaa
    49261 cgacttatga aactaaagac cacattgttc ggcaatgtat atcagtttaa ggatgtaaaa
    49321 gaggtgctgg ctaaagccaa cgaactgcgt tcgggggatg tgctggcggg cgttgcagcg
    49381 gcaagctcac aggagcgcgt ggcggcaaag caggtgttgt cggaaatgac cgtagcggac
    49441 atccgcaata atccggtgat tgcctatgaa gatgactgcg tgacgcggct gattcaggac
    49501 gatgttaacg aaacggccta caaccagatt aaaaacagga gcatcagcga actgcgtgag
    49561 tatgtgctga gcgatgaaac cagcgtggac gacattgcct ttacccgcaa agggctgacc
    49621 tcggaagtgg tcgcggcggt agcgaagatt tgctccaacg cggacctgat ctacggcgcg
    49681 aagaaaatgc cggtaatcaa aaaggccaat accaccatcg gtattccggg caccttcagc
    49741 gcccgtttgc agccaaatga cacccgtgac gacgtgcaaa gtatcgctgc acaaatctac
    49801 gaagggcttt ccttcggggt gggcgatgcg gtgatcggcg ttaacccggt aactgacgac
    49861 gtggaaaact taagccgcgt gttggacacc atttacggcg tgatcgacaa attcaacatc
    49921 ccaactcagg gctgcgtact ggcgcacgtc accacccaga tcgaagcgat tcgtcgcggc
    49981 gcgcctggcg gactgatttt ccagagtatc tgtggcagcg aaaaagggct gaaagagttt
    50041 ggcgtggaac tggcgatgct cgacgaagcg cgcgcagtgg gtgctgagtt caaccgtatc
    50101 gccggggaaa actgcctcta cttcgaaacc gggcaaggtt cggcgctgtc tgctggcgct
    50161 aacttcggcg cagaccaggt gacgatggaa gcacgtaact atgggctggc gcgtcattac
    50221 gatccgttta tcgtcaacac cgtggtcggc tttattgggc cggagtatct ctacaacgac
    50281 cgccagatta tccgtgctgg cttagaagat cactttatgg gcaagctgag cggcatctct
    50341 atgggctgtg actgctgtta caccaaccac gctgacgctg accagaacct caacgaaaac
    50401 ctgatgatcc tgctcgccac cgcaggctgc aactacatca tggggatgcc gctgggcgat
    50461 gacatcatgc tcaactatca gaccaccgca ttccacgaca ctgccactgt gcgtcagtta
    50521 ctcaacctgc gcccgtcacc ggagtttgaa cgctggctgg aaagcatggg cattatggca
    50581 aacggtcgcc tgaccaaacg ggcgggcgat ccgtcactgt tcttctgatg acgcggagat
    50641 aacacatcat ggatcaaaaa cagattgaag aaattgtacg cagcgtgatg gcgtcaatgg
    50701 gacaagcggc ccccgcgccg tcagaagcaa agtgcgccac cactaactgt gcggcaccgg
    50761 tgacctcgga aagctgcgcg ctggatttag gttctgcgga agcaaaagcg tggattggtg
    50821 ttgaaaaccc gcatcgcgca gacgtattaa cagaactgcg ccgcagcacc gtggcccgcg
    50881 tttgtaccgg tcgtaccggt ccgcgtccac gtacgcaggc gctgctgcgt ttcctggccg
    50941 atcactctcg ttcgaaagat accgtactga aagaagtgcc ggaagagtgg gtgaaagcgc
    51001 agggcttgct ggaagtacgc tccgaaatta gcgacaaaaa tctctacctg actcgcccgg
    51061 atatgggccg ccgcctgtgt gcagaagctg ttgaagcgct gaaagcacag tgtgttgcta
    51121 atccggacgt acaggtcgtt atttctgatg gcctgtcaac tgatgccatc accgtgaact
    51181 acgaagagat cctgccgccg ctgatggcgg gcctgaaaca ggccgggctg aaagtcggca
    51241 cgccattctt tgttcgttat ggtcgcgtga agattgaaga tcagatcggt gagatcctcg
    51301 gcgcgaaagt ggtgatcctg ctggtggggg aacgtccggg gttgggtcag tcagaaagcc
    51361 tctcctgtta cgccgtttac tcgccgcgta tggcgaccac cgttgaggcc gatcgcacct
    51421 gcatctctaa cattcaccag ggcggcacgc cgccggttga agccgcagcc gtcattgtgg
    51481 atttggcgaa acgtatgctg gagcagaaag catccggcat caacatgacc cgataaggag
    51541 gcatcatgcc agctttagat ttgattcgac cgtcggtaac cgccatgcgg gtgattgcct
    51601 ccgttaacgc tgaatttgcg cgtgaactaa aattgccgcc gcatattcgt agcctcgggc
    51661 tgatttctgc tgattccgat gacgtcacct acattgcggc tgacgaagcg accaagcagg
    51721 cgatggtcga agtggtatat ggccgctcgc tgtatgcggg agcagcacac ggaccgtcac
    51781 cgactgccgg tgaagtgctg attatgcttg gtgggccaaa cccggcggaa gtgcgtgctg
    51841 gtctggatgc gatggttgcg catattgaaa atggtgctgc tttccagtgg gctaatgacg
    51901 cagaaaacac cgcattcctg gcacatgtag tttcgcgtac cggttcttat ctttcatcaa
    51961 ccgccgggat cacgcttggc gatccgatgg cgtatctggt ggcaccgccg ctggaagcga
    52021 cctacggcat tgatgcagcg ttgaaatctg ccgacgtaca gctggtgacc tatgtcccgc
    52081 caccgtctga aaccaactac tcggcagcat ttttaaccgg tagccaggcc gcgtgtaaag
    52141 cagcctgtaa cgcctttacc gatgcagtgc tggaaatcgc gcgtaatcca atccagcgtg
    52201 cgtaacggag gttgccgatg atcaatgcac tgggattgct ggaagtggac ggcatggtcg
    52261 ccgcgataga tgcagcggat gccatgctca aagcagctaa cgttcgtctg ctcagtcacg
    52321 aagtgcttga ccctggtcgc ttaacgctgg tggtggaagg cgatctggcg gcgtgtcgtg
    52381 cggcgctgga cgcaggttgt gctgccgcga tgcgtaccgg gcgtgtcatc agccgcaagg
    52441 agatcggtcg gccagacgat gacacccagt ggctggttac tggctttaac cgccagccga
    52501 agcaacccgt aaaggaaccc gacgcgccag ttatcgtcgc ggaatctgct gacgagttgt
    52561 tggcgctgtt aacatcagta cgtcagggaa tgacggcagg agaagtggct gcccactttg
    52621 gctggccgct ggaaaaagcc agaaatgcgc tcgaacagct cttttctgcc gggacgttac
    52681 gtaaacgcag tagtcgttat cgtctcaagc cccattaacc tgtcggaggt gccgggtgtc
    52741 ctacaacacc cggcattaac atcatgaaaa agacccgtac agccaatttg caccatcttt
    52801 atcatgaacc cttacccgaa aacctgaagc tcacgccgaa ggtcgaagtg gataatgttc
    52861 atcaacgaca gacaacggat gtctatgaac atgctttgac gattaccgcc tggcagcaga
    52921 tttacgatca gctgcatccg ggcaagtttc atggtgaatt tacggaaatt ctgctcgatg
    52981 atattcaggt ttttcgtgaa tacaccggtc tggcgctgcg tcagtcgtgc ctggtctggc
    53041 cgaactcgtt ctggtttggc attccggcga cgcgcggtga gcagggattt atcggttcgc
    53101 aatgtctggg aagcgcagaa atagcgacgc gccccggtgg aaccgagttt gaattaagta
    53161 cgccggacga ttatacgatc ctgggcgtag tgctgtctga agatgtcatc acccgtcagg
    53221 ctaacttttt gcacaatccg gatcgggtat tacatatgct gcgtagccag tcggcgctgg
    53281 aagtgaaaga gcagcataaa gccgcgctgt ggggctttgt ccaacaggcg ctggcgacgt
    53341 tttgcgagaa cccggaaaat ctccatcagc ctgcagtgcg aaaagtgctg ggggataatt
    53401 tgctaatggc gatgggggcg atgctggaag acgcgcaacc aatggtgacg gcggaaagca
    53461 tcagtcatca gagttaccgt agattacttt cccgcgcccg tgaatatgtg ctggaaaata
    53521 tgtctgagcc ggtgacggtg ctggacttgt gtaatcaact gcatgtcagt cgccgcacgc
    53581 tacaaaacgc gtttcacgct attttaggca ttggcccaaa cgcgtggctg aaacgcattc
    53641 gcctgaacgc cgtacgccgc gaactgataa gcccgtggtc gcaaagcaca acggtaaaag
    53701 acgccgccat gcagtgggga ttctggcatc tggggcaatt tgccacggat taccagcagc
    53761 tgtttgccga gaagccgtcg ttgacgttgc atcagcggat gcgggaatgg gggtgaggga
    53821 gttacaccca atccctgacc ttaataaact cacttaacgc cgcttctggg ctgccatctt
    53881 ttggctgata atcatactcc cagcgtacca gtggcggcat tgacatcagg atcgactcgg
    53941 tgcgcccgcc agtttgcagg ccaaacagag tgccgcgatc ccagaccaga ttgaactcga
    54001 cataacgacc gcgacggtag agctgaaaat tgcgctcgcg ctcgccgtag gccatcgctt
    54061 tacgtcgctc tacaattggt aaataagcgt cggtgtagcc tttgcctacc gcctgcataa
    54121 aggcaaaaca gtggtcgaaa tctggcgtgt tcagatcatc aaagaacagc ccgccaatac
    54181 cgcgctgttc gttgcgatgt ttgaggtaga agtaatcgtc gcaccacttt ttgtaacggg
    54241 gataaacgtc ttcaccaaat ggcaggcaca ggtcacgggc ggtgcggtgc cagtgaatgg
    54301 cgtcttcttc aaaaccatag aaaggggtta aatcgaagcc gccgccaaac caccagacgg
    54361 gctctgcacc cggtttttcg gcaataaaaa aacgcacatt cgcgtggctg gtgggaacat
    54421 acgggttatg cggatgcact accagtgaaa cgcccatcgc ctcgaaactg cgcccggcaa
    54481 gttccgggcg atgagcggtg gcggaagcag gcatcgcctc gccgtggacg tgagaaaagt
    54541 tgacgcctgc ctgttcgaaa acaccaccat tacgcaacac ccgactacgc ccgccgccgc
    54601 cagcttcgcg ctgccaacta tcttcgacaa attctgcgcc atcgacggcg gtcagctgct
    54661 gacaaatcgt atcctgaagg ttgagcagaa actgtttaac ctggtgtgcg tcgggtttca
    54721 taacttatcg ctttttcgaa tgtgctttct ggttgtcgaa ccagtggaaa taactgatca
    54781 cgccttcagc aatcgctgtg gcgattttct gacgaaacgc cgccgtgcct aacagccgct
    54841 cttcttccgg gttggtgata aacgaggttt ccaccagcac cgaaggaacc gacggcgatt
    54901 tcaacaccac aaacgcggct tgttcggtgt tgcggctgtg cagtttatgc accggcttaa
    54961 tctttttcag aatatgcgag ccaagcgtca ggctgttttt aatagtatcg gtttgcacca
    55021 gatcaaacag cacttgctgc aataggtgat ccttgtcagt cgccttttta ccggcaactt
    55081 catcggcgcg gttttcgcgt tcagacaggt atttcgccat tgcgctactt gccccacggt
    55141 tagagagggc aaataccgaa gcaccggcag ctttcgggtt ggtaaagcca tcggcatgaa
    55201 ttgacataaa cagatctgcg ccatgtttat gggcgatttc aacgcgatcg taaagtggga
    55261 taaacgtatc gccagaacgc gttaaacgcg catcaatccc atgattacgc aaaatggaac
    55321 ggacgttttt agcaatcgcc agcaccacat gtttttcttt cgaaccgttg cgtccgatcg
    55381 ctccggtatc aattccgccg tggcctggat cgagaacaac gacacgtttg ccgccagatt
    55441 ttttggcttt cggcttgctg tgtccgttgc tggtttttaa aggttcgtct ttggcgatgg
    55501 cttgcgacat tcctgacaac gtcagggcag ccaaaccggc tttcagcacc tggcggcgcg
    55561 aagtgagtgt ttttagtggt ttaaaagtgc tcatacggcc tgagttgtaa taataaagtt
    55621 ccagatgtta tatcgtatcg cgtaatccgt tacgacggtt tgatgttgag aattgtttta
    55681 cttttcattt caatagttga caactcccca ttatgccact ttttcaaccg attttcgtca
    55741 gatattggct aattcgaccg ttcacgccat aatcagcctt ttaacccgca aaaaggtaac
    55801 gaataccatg gagatacgcg tatttcgcca ggaagatttc gaagaggtca tcaccctttg
    55861 ggagcgttgc gacttgctgc gtccgtggaa cgatccggaa atggacatcg agcgtaagat
    55921 gaaccatgac gtcagtttgt ttttggtcgc tgaggtaaac ggtgaagtgg tcggaacggt
    55981 gatgggcggt tatgacgggc atcgcgggtc tgcttattat cttggcgtgc atccagagtt
    56041 tcgtgggcgt gggattgcca atgcgttgct taatcggctg gagaaaaagc tgattgctcg
    56101 tggctgcccg aaaattcaga tcaatgtgcc ggaagataac gacatggtgc tcggaatgta
    56161 tgaacgcctg ggatatgaac acgccgacgt gctgagtctg ggtaagcgtt tgattgaaga
    56221 tgaagagtac tgagttccat cctgtccatt atgatgcgca cggacgcctg cgtttaccct
    56281 tgctcttctg gcttgtgcta ttgcttcagg cgcgaacctg ggtgctgttt gtcattgccg
    56341 gtgcgtcgcg cgagcagggt actgcgctgc taaatctgtt ttatcccgat cacgataatt
    56401 tctggctggg gctgattcca ggcattccgg cggtgctggc gtttttgttg agcggtcggc
    56461 gggctacatt tcctcgcact tggcatgtgc tctattttct gttgctgttg gcgcaggtgg
    56521 ttttactttg ctggcaaccg tggctgtggc tgaacggtga atccgttagc ggtatcggtc
    56581 tggcgctggt agtggcggat attgtggcgt taatctggct gctgaccaat cgacgtttac
    56641 gcgcttgttt taatgaagaa aaagaataac ggcacttttt ggtgaatttg cactccaagc
    56701 aacgttattg aataaccaaa ggaagtgaca tgaaatcgct gcgtttaatg ttatgcgcta
    56761 tgccgttgat gctgaccggc tgttccacga tgtcgtcagt taactggtcg gccgctaacc
    56821 cgtggaactg gtttggatcg tccaccaaag tgagcgagca gggcgtgggt gaattaacgg
    56881 cgtccacacc actgcaagaa caagccattg ctgatgcgct tgatggcgat tatcgcctgc
    56941 gcagcggaat gaaaaccgcg aacggcaacg tggtgcgctt ttttgaagtg atgaaaggcg
    57001 acaacgtggc gatggtgatt aacggcgatc aaggcacgat cagtcgcatt gacgtgctgg
    57061 atagcgatat tcctgctgac accggtgtta aaatcggtac accgtttagc gacctttaca
    57121 gcaaagcatt tggcaattgc caaaaagctg atggtgatga taatcgtgcc gtcgaatgta
    57181 aagccgaagg cagtcaacat attagctacc agttcagcgg ggaatggcgt ggtcctgaag
    57241 ggttaatgcc ttcggacgat accctgaaaa actggaaagt cagtaaaatt atctggcggc
    57301 gttaatttgc gtctgaacaa accgccgccg cgaaaaaaca cgtaaaatag cgctcaacaa
    57361 tgccacggat tgcgtggcat tcttattttc aggaggaaca atgtctcagg ttcagagtgg
    57421 cattttgcca gaacattgcc gcgcggcgat ttggatcgaa gccaacgtga aaggggaagt
    57481 tgacgccctg cgtgcggcca gtaaaacatt tgccgacaaa ctggcaactt ttgaagcgaa
    57541 attcccggac gcgcatcttg gtgcggtggt tgcctttggt aacaacacct ggcgcgctct
    57601 gagcggcggc gttggggcag aagagctgaa agattttccg ggctacggta aaggccttgc
    57661 gccgaccacc cagttcgatg tgttgatcca cattctttct ctgcgtcacg acgtaaactt
    57721 ctctgtcgcc caggcggcga tggaagcctt tggtgactgc attgaagtga aagaagagat
    57781 ccacggcttc cgttgggtgg aagagcgtga cctgagcggc tttgttgacg gtacagaaaa
    57841 cccggcgggt gaagagacgc gtcgcgaagt ggcggttatc aaagacggcg tggatgccgg
    57901 cggcagctat gtgtttgtgc agcgttggga gcacaacctg aagcagctca accggatgag
    57961 cgttcacgat caggagatga tgatcgggcg caccaaagag gccaacgaag agattgacgg
    58021 cgacgaacgt ccggaaacct ctcacctcac ccgcgttgat ctgaaagaag atggcaaagg
    58081 gctgaagatt gttcgccaga gcctgccgta cggcaccgcc agtggcactc acggtctgta
    58141 cttctgcgcc tactgcgcgc gtctgcataa cattgagcag caactgctga gcatgtttgg
    58201 cgataccgat ggtaagcgtg atgcgatgtt gcgtttcacc aaaccggtaa ccggcggcta
    58261 ttacttcgcg ccgtcgctgg acaagttaat ggcgttgtaa gcgtttctga ttcgatgatt
    58321 aaaggccagc cagtaacaaa actgactggc ctttttatta cttttgcttc aacgccgcat
    58381 acacctgatc aactacccaa ccataagttg caatcggtaa ctgaccgctt tcgaacatat
    58441 tgggattttt ttgtggatcg gcaactggcg aatggggctt gttgcttaac atcacaatcg
    58501 ccatatgatt gaccggatcg ataacggtta ctgtcccggt ccagccagtg tgaccgtagg
    58561 tttgcgggct tgccagcgtg ccaaacgtcg gcgtcatggt ggcattacca ttcacgcgcc
    58621 agccgaggcc aaaagtggca tcttccttag agctggtggt gaacatcttc accgtttccg
    58681 cactgaacag ctgcacatcg ccatagcccc cgccgttcag catcgtttgc attaacaccg
    58741 caatatcgcc ggtattggaa aacaaacctg cgtgcccgga aacgccgccc atcgaataaa
    58801 aggctttttc atcgtgcacc tgaccccaga gagtggaggt gcggatattc gggaaatgga
    58861 tcacgccatc gcgggtattg ccgttcaatt ccgtggcggc aatttgttgc ggtttaaagc
    58921 ctttcagcag cgggttaaat accgtatgtg tcaggccgag cgggcgataa atcgactctt
    58981 caacatagcg gtcaagcggc tgaccggtaa ccgactcgac gataaatcca agcagcatat
    59041 aatcgacatc gctgtagata tgtttgctgc cgggctgata ttccagcggc gtgcgcttga
    59101 tcatctccag cgtctggcct ttatcctggg aatataacgc gcccgcgacc gctttattcg
    59161 ggtattgcgg atctgccggg aaaccgccgc tgtgatgcag caggtcagaa atccgcaggg
    59221 tgtttttgcc tttaatggtg tcatttggac tatcggcaaa ccccggaata tatttcgcaa
    59281 tccgatcgtc aggatgcagt ttgccttcgc tcatcagctt ttgcagggcg aagttcgtgg
    59341 cgtacatttt ggtgtttgag gccaggtcat acagcgtccc ggtggtggct ttgacaggct
    59401 gttccatcaa cacgctgcca tcgtactttt tcgccgctcc ccaggctttg cgatacacaa
    59461 tctgattatc tttaatgatc agcaggttta cgccgggata accgacatca atttgctggc
    59521 taatccagcg atccatctgg ttaagccgtt cgacgttaaa ccctgctttc tctggcgagc
    59581 tttctgtcag aacagggtat tttgcggcgc tgacgctaca actaacagcc agcagagaaa
    59641 gatagagcat tgtccgtttc atgatatcag tccagattga cgttacggca gccaaagagc
    59701 gtggtgaaaa taaacccgca aacccatgcc accagaatcc cgccagcata aaccgccatg
    59761 gccggaagga tgccttgtgc ggaagtcatc agcggcagcg ctaccagacc agacggccca
    59821 aaggcgctgt ttaagcccat cggcagaccc catcaggcta tcaggccgat aaacaaaccg
    59881 cccgccgcgc cgcctaaaca ggcggtaaca aacggcttca tgcggggcag ggtgacaccg
    59941 taaatcagcg gttcaccaac gcccagcagg ccgggaataa tcgccccgcg tacctgactg
    60001 cgtagcgcac tgtgcggttg cgcccgccag tagagtgcca gcgccgcgcc cacctggccc
    60061 gcgcctgcca ttgaaaggat gggaaataag ctgttgaatc cctggctgtc cattaacgcg
    60121 aggtaaacag gaataaagcc ctgatgcacg ccaaatacca cggcgatcag gaacagcccg
    60181 gctaaaaccg cacaaccgaa cggattactg ttcaggtgca taaacagcca tgacatacct
    60241 tcgaataacc agccacccag cggcataatg atcaggtacg caagcgtagc ggtgatcagc
    60301 agggtgatta acgaggtcag cagcatgtcg agatcgtccg acataaagcg gcgcaccatg
    60361 ccttcaatgc gggcgcaggc ccacgcggca atcagcacgc cgataatatt gccgcgcgga
    60421 tcgatgggca gaccaaagaa atcgtgaaaa ccggcgtagt aaccggtggt tgcagcgggg
    60481 ttataaccga gcaaaaagag tgcggcaata attgcgccat tgacgcccgt gccgccaaat
    60541 gcctgggcgg cgttatagcc caccagaatc accaggaaag tgaacaaacc tttgctgaac
    60601 accttcataa aattcagcgc gtcgggtagc gtgccctgag catctgccgg aacgtgcatc
    60661 accgtggcaa ttaacgtcgc tatccccagc agcagaccgg cggcaataaa accggggatc
    60721 agcggcgtga agatggtggc gaatttggcg agaaattgtt gcacgccgga ggtttgtttg
    60781 gcttttaact gacgtttgtt ctgggcggcg atttctgcgg catcctgtac cggtgcctcg
    60841 cccagcagct cgctcatcgc ttttgcggca cgatgcgctt taccaggccc aaaaacgacc
    60901 tgcacctggt cgctggtcaa aatgacgccc ttcacgcctt ccagcgtttt gatattggga
    60961 tcaaccagtg aactgtcatg tacacccaga cgcaggcgcg tcatacagtt accacaactg
    61021 gcgatatttc ccagtccgcc gacacgggta agaatggtgt tcagaagttc actgctgatc
    61081 tctttggcca tgggttattc cttgtctaaa acctgacgaa taaagccgcc gtgttgatcc
    61141 aggcgttttt tagcttctgc ggcatcgaga tttttcagca ccatcacaat ggccgtttta
    61201 cagttgcgct cgcaggcaat taacgccgct tccgcttgtt cggcattaca tccggtggcg
    61261 tttttaacga tattgacctg gcgcacatgc agtttttcgt tggtggcgac cacatcgacc
    61321 atcaggttgc cgaacacttt gccggattta atcatcagcc cggtggaaag catattgagc
    61381 accagtttct gcgctgtgcc tgctttcatt cgcgaagaac cagtgaccac ttcggcacct
    61441 acaatcggtg taatggcaaa ctcggcggtg gtggaaacgg cactccccgg attacaggaa
    61501 attcccactg tgcggcaacc gagctggcgg gcgtattcca gtccggcaat cacatacggc
    61561 gtacgaccgc tggcggcaat accgaccacc acatcctgtg ccgttaaatt tatatttttc
    61621 agatcgttaa tgccgccttc tcggctatct tccgtgcctt ccaccgcgtg ctgaatggca
    61681 tattcgccgc cagcaatcaa accaaccacc agacccggtt tcacgccgta ggtgggcgga
    61741 cattcgctgg catccagaat ccccagacga ccggatgttc ccgcaccgag ataaatcaga
    61801 cgaccgccgc cgcttacctg ggcgtggata acatcgatcg ccgcggcgat atccggcagg
    61861 acgcgctcaa cggcaagcgg tacggtttta tcttcatcgt tgataatccg gcacatttcc
    61921 agcgtcgata cgcggtcaat ttcagccgag gcggtgttcg agccttcagt aatcatcttt
    61981 tcaagttgca tcggtggtga ccttactatt tacgatcaaa taattgcaga tgtatggaat
    62041 catatatccc gaattgacag acttaaagga atcattgagt ctacacttgc agagtgtgaa
    62101 gagcgtcata agaacttaag cggaattttt gctgccagcc cgggagagtg gaagatgttg
    62161 tacctgacga aaatacgcaa tgcggaaagt gaatttaccg agaacgagca aaaaattgct
    62221 gactttctgc gggccaacgt gagtgagctg aaatccgtct cttcgcgcaa gatggcgaaa
    62281 cagctcggca tcagtcagtc gagcattgtg aaatttgccc agaagctcgg cgcgcaagga
    62341 tttactgaat tgcgtatggc gttaattggc gaatacagtg ccagccggga gaaaacgaat
    62401 gccacggcgc tgcacctgca cagttcgatt accagtgatg attcgctgga agtgattgcc
    62461 cgcaaactga atcgcgaaaa agagctggcg ctggagcaaa cctgtgcgtt gttcgactac
    62521 gctcggctgc aaaaaatcat tgaggtcatc agtaaagcac cgtttatcca gataacgggc
    62581 ctgggcgggt cggcgctggt ggggcgcgat ctgtcgttca aactgatgaa aattggctat
    62641 cgcgtggcct atgaagccga cacacacgta caagccaccg tttcccaggc gttgaaaaaa
    62701 ggcgatgtac aaatcgctat ctcttacagc ggcagtaaaa aagagattgt cttgtgcgca
    62761 gaagcggcac gaaagcaggg ggcaacggtg attgccatta cttctctggc tgactctccg
    62821 ctgcggcggc tggcgcattt cacgctcgat accgtttccg gtgaaaccga atggcgtagc
    62881 tcgtcgatgt ctacccgcac ggcgcaaaat tctgttaccg atttactgtt tgttggtctt
    62941 gtgcaactca atgatgtgga gtcattgaaa atgattcagc gcagcagtga gctaacacaa
    63001 cgcctgaaat gatgcataaa gcagcgactg gattgagatt ttcctgaatt agtgagctga
    63061 tccgcagcaa tattttgttt atcctgtatt ttcagaggga atggagtgta acgctctgta
    63121 ttaacaagga gagcattaaa atgggtaaac tcacgggcaa gacagcactg attacgggcg
    63181 cattgcaggg aattggcgaa ggaattgcca gaacttttgc gcgtcatggc gcaaacctaa
    63241 tcttgctgga tatctcccct gagatcgaaa agctggcgga cgaactgtgt ggtcgtggtc
    63301 atcgctgtac ggcggttgtc gccgatgtgc gtgacccggc gtcggtagcc gcagctatca
    63361 aacgcgcgaa ggaaaaagaa gggcgcattg atatcctggt gaataacgca ggcgtttgtc
    63421 gtctgggcag tttcctcgat atgagcgatg acgatcgcga tttccatatt gacatcaata
    63481 ttaaaggcgt atggaacgtc acgaaggcgg tgctgccgga gatgattgcc cgcaaagatg
    63541 gtcgcattgt gatgatgtct tcagtcactg gtgatatggt ggccgatcct ggcgaaacgg
    63601 cgtacgcctt aacgaaagcg gcgattgttg gcctgacaaa atcgctggcg gtggagtacg
    63661 cgcagtctgg tattcgcgtt aacgccattt gcccgggata cgtgcgcaca ccaatggcgg
    63721 aaagcattgc ccgccagtcg aacccggaag atccagagtc ggtgctgact gaaatggcga
    63781 aagcaatccc gctgcgtcgc ctcgccgatc cgctggaagt cggcgaactg gcggccttcc
    63841 tcgcatcgga tgaatccagc tatttaaccg gtacacagaa tgtgattgat ggcggcagca
    63901 cactgccgga gacggttagc gtcggtatct gattcacctc tgtttcctcc ctgcatttgt
    63961 ggggaggatt tcgttttaaa cccggttctt attccctttt caacttccaa atcaccaaac
    64021 ggtatataaa accgttactc ctttcacgtc cgttataaat atgatggcta ttagaaagtc
    64081 attaaattta taagggtgcg taatggccgt taacttactg aaaaagaact cactcgcgct
    64141 ggtcgcttct ctgctgctgg cgggccatgt acaggcaacg gaactgctga acagttctta
    64201 tgacgtctcc cgcgagctgt ttgccgccct gaacccgccg tttgagcaac aatggaccaa
    64261 agaaaacggc ggcgacaaac tgacaataaa acaatctcat gccgggtcat caaaacaggc
    64321 gctggcaatt ttgcagggct taaaagcaga cgttgtcact tataaccagg tgactgacgt
    64381 acaaatcctg catgacaaag gcaagctgat cccggcggac tggcaaacgc gcctgccaaa
    64441 taatagctcg ccattctact ccaccatggg cttcctggtg cgtaagggca acccgaagaa
    64501 tatccacgac tggaacgacc tggtgcgctc cgatgtgaag ctgattttcc cgaacccgaa
    64561 aacgtcgggt aacgcgcgtt acacctatct ggcggcatgg ggcgcagcgg ataaagctga
    64621 cggtggcgat aaagccaaaa ccgaacagtt tatgacccag ttcctgaaaa acgttgaagt
    64681 gttcgatact ggcggtcgtg gcgcgaccac cacttttgcc gagcgcggcc tgggcgatgt
    64741 gctgatcagc ttcgagtcgg aagtgaacaa catccgtaaa cagtatgaag cgcagggttt
    64801 tgaagtggtg attccgaaaa ccaacattct ggcggaattc ccggtggcgt gggttgataa
    64861 aaacgtgcag gccaacggta cggaaaaagc cgccaaagcc taccttaact ggctctacag
    64921 cccgcaggcg caaaccatca tcaccgacta ttactaccgc gtgaataacc cggaagtcat
    64981 ggataaactg aaagacaaat tcccgcagac cgagctgttc cgcgtggaag acaaatttgg
    65041 ctcctggccg gaagtgatga aaacccactt caccagcggc ggcgagttag acaagctgtt
    65101 agcggcgggg cgtaattaat gtttgcagtt tcatcaagac gcgtgctgcc gggctttacc
    65161 ttaagcctcg gcaccagtct gctgtttgtg tgcctgattt tgctgctgcc gctctccgcg
    65221 ctggtgatgc agctggctca gatgagctgg gcgcagtact gggaggtgat caccaacccg
    65281 caggtagtag cggcctacaa agtaacgctg ctgtcagcgt ttgtggcatc gatttttaac
    65341 ggcgtttttg gcctgctgat ggcgtggatc ttaacccgct atcgctttcc gggccgcacg
    65401 ctgcttgatg cgctgatgga tttacccttt gcgctgccaa cggcagtcgc cggtttaacg
    65461 ctagcctcgc tcttttctgt aaacggtttt tacggtgaat ggctggcgaa gtttgatatc
    65521 aaagtcacct atacctggct ggggattgcg gtggcgatgg cctttaccag cattccgttt
    65581 gtggtacgta ccgtgcagcc ggtgctggaa gagttaggcc cggaatatga agaagcggca
    65641 gaaacgcttg gcgcaacgcg ctggcagagt ttctgcaaag tggtgctgcc ggaactttct
    65701 ccggcgctgg tggcgggcgt ggcgctgtcg tttacccgta gtcttggtga atttggcgcg
    65761 gtgattttta tcgccggaaa tatcgcgtgg aagacggaag tgacgtcgct gatgattttt
    65821 gtgcgcttac aggagtttga ttacccggca gcgagcgcga ttgcttcggt gatcctcgcg
    65881 gcatctctgc tgctgctgtt ctcaattaac actctgcaaa gtcgctttgg tcggcgtgtg
    65941 gtaggtcatt aatggcggaa gttacccaat tgaagcgtta tgacgcgcgc ccgattaact
    66001 ggggcaaatg gtttctgatt ggcatcggga tgctggtatc ggcgttcatc ctgctggtgc
    66061 cgatgattta tatcttcgta caggcgttca gcaaagggct gatgccggtt ttacagaatc
    66121 tggccgatcc ggacatgctg cacgccatct ggctgacggt gatgatcgcg ctgattgccg
    66181 taccggtaaa cctggtgttc ggcattctgc tggcctggct ggtgacgcgc tttaacttcc
    66241 ctggacgcca gttactgctg acgctactgg acattccgtt tgccgtatcg ccggtggttg
    66301 ccggtctggt gtatctgctg ttctacggct ctaacggccc gctgggcggc tggctggatg
    66361 aacataatct gcaaattatg ttctcctggc ccggaatggt gctggtcacc atctttgtga
    66421 cgtgtccgtt tgtggtgcgc gagctggtgc cggtgatgtt aagccagggc agccaggaag
    66481 acgaagcggc gattttgctt ggcgcgtctg gctggcagat gtttcgccgc gtcacattgc
    66541 cgaacattcg ctgggcgctg ctttatggcg tggtgttgac caacgcccgc gcaattggcg
    66601 agtttggcgc ggtgtcggtg gtttccggct cgattcgcgg cgaaaccctg tcgctgccgt
    66661 tacagattga attgctggag caggactaca acaccgtcgg ctcctttacc gctgcggcac
    66721 tgttaacgct gatggcgatt atcaccctgt ttttaaaaag tatgttgcag tggcgcctgg
    66781 agaatcagga aaaacgcgca cagcaggagg aacatcatga gcattgagat tgccaatatt
    66841 aagaagtcgt ttggtcgcac ccaggtgctg aacgatatct cactggatat tccttcaggt
    66901 cagatggtcg cgttgctggg gccgtccggt tccgggaaaa ccacgctgct gcgcattatc
    66961 gccgggctgg agcatcaaac cagcgggcat attcgcttcc acggcaccga cgtcagccgc
    67021 ctgcacgcgc gcgatcgcaa ggtcggtttc gtgttccagc attacgcgct gttccgccat
    67081 atgacggtat tcgacaatat cgcttttggc ctgacggtgc tgccgcgtcg cgatcgcccg
    67141 aatgccgcag ccatcaaagc gaaagtgaca aaattgctgg agatggtgca gcttgctcat
    67201 ctggcggatc gttatccggc acagctttcc ggcggccaga aacagcgcgt ggcgctggcg
    67261 cgcgcgctgg ctgtagaacc gcaaattctg ctgcttgatg aaccgtttgg cgcgctggat
    67321 gcgcaggtgc gtaaagagct gcgtcgctgg ctgcgtcaac tgcatgaaga gctgaaattc
    67381 accagcgtgt tcgtgaccca cgaccaggaa gaagcgacgg aagtagctga tcgtgtagtg
    67441 gtgatgagcc agggcaatat tgaacaggct gatgcgccgg agcaggtatg gcgcgaacca
    67501 gcgacccgtt ttgtgctcga atttatgggc gaagtgaacc gcctgcaggg aaccattcgc
    67561 ggcgggcagt tccatgttgg cgcacatcgc tggccgctgg gctacacacc tgcttatcag
    67621 gggccggtgg atctcttcct gcgcccgtgg gaagtggata tcagccgccg taccagtctc
    67681 gattcgccgc tgccggtaca ggtactggaa gccagcccga aaggtcacta cacccaatta
    67741 gtggtgcagc cgctggggtg gtacaacgaa ccgctgacgg tcgtgatgca tggcgacgat
    67801 gccccgcagc gtggcgagcg tttattcgtt ggtctgcaac atgcgcggct gtataacggc
    67861 gacgagcgta tcgaaacccg cgatgaggaa cttgctctcg cacaaagcgc ctgataggtt
    67921 gagtgaatgt taaacgcccg gaggcgcttc ccgcgatccg ggctttttaa tggcaaggtt
    67981 tgtaacctgt agacctgata agacgcgcaa gcgtcgcatc aggcaacacc acgtatggat
    68041 agagatcgtg agtacattag aacaaacaat aggcaatacg cctctggtga agttgcagcg
    68101 aatggggccg gataacggca gtgaagtgtg gttaaaactg gaaggcaata acccggcagg
    68161 ttcggtgaaa gatcgtgcgg cactttcgat gatcgtcgag gcggaaaagc gcggggaaat
    68221 taaaccgggt gatgtcttaa tcgaagccac cagtggtaac accggcattg cgctggcaat
    68281 gattgccgcg ctgaaaggct atcgcatgaa attgctgatg cccgacaaca tgagccagga
    68341 acgccgtgcg gcgatgcgtg cttatggtgc ggaactgatt cttgtcacca aagagcaggg
    68401 catggaaggt gcgcgcgatc tggcgctgga gatggcgaat cgtggcgaag gaaagctgct
    68461 cgatcagttc aataatcccg ataaccctta cgcccattac accactaccg ggccggaaat
    68521 ctggcagcaa accggcgggc gcattaccca ttttgtctcc agcatgggaa caaccggcac
    68581 tatcaccggc gtttcacgct ttatgcgcga acaatccaaa ccagtaacga ttgttggcct
    68641 gcagccggaa gaaggcagca gtattcccgg cattcgccgc tggcctgcgg aatatctgcc
    68701 ggggattttc aacgcttctc tggtggatga ggtgctggat attcatcagc gcgatgcaga
    68761 aaacaccatg cgcgaactgg cagtgcggga aggaatattc tgtggcgtca gctccggcgg
    68821 cgcggttgcc ggagcactgc gggtggcaaa agctaaccct ggtgcggtgg tggtagcgat
    68881 catctgcgat cgtggcgatc gctacctttc caccggggtg tttggggaag agcattttag
    68941 tcagggggcg gggatttaag attattgcgc aggcaccgtt ttatccgtct gcgcaattaa
    69001 ggcatgtaaa aaaggctgtg cgttctcatc gctcttaccg gggatcttca caatatacgg
    69061 cttcccggta atcgatgacg acgaagcaac cttatcaata aactgctcgg cagtgtcaat
    69121 gcggttacgg gtattgccga gcttcagacg cagatgagaa accgcttcat cgcaggtatg
    69181 ttcatcaccg ttgcgcacaa atatcaaatc ctttttctgt gctaatccct ccagcatggc
    69241 gttgatgcgg gcttcttcat gaacagttaa cttcgcgtaa gcggggagtg tcattagtag
    69301 tgtaatgacc agacaaatga ttttcttcat tgaattacct tatccttgtt ttgtgatagc
    69361 cgatttgact gccagaatca ggtaaagttt cggttggaat acgatgagga tcatcggcaa
    69421 ggagaaaaaa ttaaatgagt agtttgttgt tgtttaacga taagagtagg gcgctgcagg
    69481 cggatatcgt cgccgtgcag tcgcaggtag tgtacggcag cgtaggcaac agcattgcgg
    69541 tgcctgctat aaaacagaac ggcttgaatg tctttgccgt gccgacggta ttgctgagca
    69601 atacgccgca ttatgacact ttctacggtg gtgcgattcc ggacgaatgg tttagcggct
    69661 atttgcgtgc gcttcaggag cgtgatgcgc tgcgccaact tcgtgctgta accacgggct
    69721 atatgggaac ggcatcgcaa atcaaaatcc ttgccgagtg gctgactgcg ctacgcaaag
    69781 accattctga cctattgatc atggtcgatc cggtgattgg cgatattgat agcggaattt
    69841 atgtcaaacc tgaccttccc gaggcgtatc gacaatattt actgccgctg gcgcaaggaa
    69901 ttacccccaa tatctttgag ctggaaatcc tgaccggtaa aaattgccgc gatctcgaca
    69961 gtgccattgc tgccgcaaaa agtctgcttt cagacacatt aaaatgggtg gtgattacca
    70021 gcgcctccgg taatgaagaa aatcaggaga tgcaggtggt ggtggtcagt gccgacagcg
    70081 tgaatgtcat ttcccattca cgggtaaaaa ccgacctgaa agggactggc gacctgtttt
    70141 gtgctcagct tatcagtggt ttgctgaaag ggaaggcgtt aaacgatgcg gtgcaccgtg
    70201 cggggttgcg cgtactggaa gtgatgcgct acacccagca gcatgagagc gatgaattga
    70261 ttttgccgcc gctggcggaa gcataaaaaa tggcgccgat gg
//
LOCUS       DAKISA010000009        64677 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.009, whole genome shotgun sequence.
ACCESSION   DAKISA010000009 DAKISA010000000
VERSION     DAKISA010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64677
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.009"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(35..1333)
                     /gene="eno"
                     /locus_tag="OVW45_000605"
     CDS             complement(35..1333)
                     /gene="eno"
                     /locus_tag="OVW45_000605"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCV5525130.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(1421..3058)
                     /gene="pyrG"
                     /locus_tag="OVW45_000606"
     CDS             complement(1421..3058)
                     /gene="pyrG"
                     /locus_tag="OVW45_000606"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCV5525131.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3286..4077)
                     /gene="mazG"
                     /locus_tag="OVW45_000607"
     CDS             complement(3286..4077)
                     /gene="mazG"
                     /locus_tag="OVW45_000607"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCV5525132.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4148..4483)
                     /gene="mazF"
                     /locus_tag="OVW45_000608"
     CDS             complement(4148..4483)
                     /gene="mazF"
                     /locus_tag="OVW45_000608"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HCV5525133.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(4483..4731)
                     /gene="mazE"
                     /locus_tag="OVW45_000609"
     CDS             complement(4483..4731)
                     /gene="mazE"
                     /locus_tag="OVW45_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HCV5525134.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(4809..7043)
                     /locus_tag="OVW45_000610"
     CDS             complement(4809..7043)
                     /locus_tag="OVW45_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCV5525135.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7091..8392)
                     /gene="rlmD"
                     /locus_tag="OVW45_000611"
     CDS             complement(7091..8392)
                     /gene="rlmD"
                     /locus_tag="OVW45_000611"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCV5525136.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPGRETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVMACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8449..11205
                     /gene="barA"
                     /locus_tag="OVW45_000612"
     CDS             8449..11205
                     /gene="barA"
                     /locus_tag="OVW45_000612"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCV5525137.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11437..12777)
                     /gene="gudD"
                     /locus_tag="OVW45_000613"
     CDS             complement(11437..12777)
                     /gene="gudD"
                     /locus_tag="OVW45_000613"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCV5525138.1"
                     /translation="MSSQFTTSVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPYGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12798..14138)
                     /locus_tag="OVW45_000614"
     CDS             complement(12798..14138)
                     /locus_tag="OVW45_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCV5525139.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14140..15492)
                     /gene="gudP"
                     /locus_tag="OVW45_000615"
     CDS             complement(14140..15492)
                     /gene="gudP"
                     /locus_tag="OVW45_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCV5525140.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(15927..16376)
                     /locus_tag="OVW45_000616"
     CDS             complement(15927..16376)
                     /locus_tag="OVW45_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCV5525141.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16394..17176)
                     /gene="truC"
                     /locus_tag="OVW45_000617"
     CDS             complement(16394..17176)
                     /gene="truC"
                     /locus_tag="OVW45_000617"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCV5525142.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17176..17505)
                     /locus_tag="OVW45_000618"
     CDS             complement(17176..17505)
                     /locus_tag="OVW45_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCV5525143.1"
                     /translation="MTTHDRVRLQLQTLEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18127..18672)
                     /gene="syd"
                     /locus_tag="OVW45_000619"
     CDS             complement(18127..18672)
                     /gene="syd"
                     /locus_tag="OVW45_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCV5525144.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQSTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18740..19588
                     /gene="queF"
                     /locus_tag="OVW45_000620"
     CDS             18740..19588
                     /gene="queF"
                     /locus_tag="OVW45_000620"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCV5525145.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19700..21064
                     /gene="ppnN"
                     /locus_tag="OVW45_000621"
                     /gene_synonym="ygdH"
     CDS             19700..21064
                     /gene="ppnN"
                     /locus_tag="OVW45_000621"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCV5525146.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLFLTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            21620..22909
                     /gene="sdaC"
                     /locus_tag="OVW45_000622"
     CDS             21620..22909
                     /gene="sdaC"
                     /locus_tag="OVW45_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCV5525147.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            22967..24334
                     /gene="sdaB"
                     /locus_tag="OVW45_000623"
     CDS             22967..24334
                     /gene="sdaB"
                     /locus_tag="OVW45_000623"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCV5525148.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            24446..25201
                     /gene="xni"
                     /locus_tag="OVW45_000624"
     CDS             24446..25201
                     /gene="xni"
                     /locus_tag="OVW45_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCV5525149.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(25306..26454)
                     /gene="fucO"
                     /locus_tag="OVW45_000625"
     CDS             complement(25306..26454)
                     /gene="fucO"
                     /locus_tag="OVW45_000625"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCV5525150.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(26482..27129)
                     /gene="fucA"
                     /locus_tag="OVW45_000626"
     CDS             complement(26482..27129)
                     /gene="fucA"
                     /locus_tag="OVW45_000626"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCV5525151.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYVTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            27676..28992
                     /gene="fucP"
                     /locus_tag="OVW45_000627"
     CDS             27676..28992
                     /gene="fucP"
                     /locus_tag="OVW45_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCV5525152.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNHTLFLVCLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPTLCFAVIFIFARFRSQTATN"
     gene            29025..30800
                     /gene="fucI"
                     /locus_tag="OVW45_000628"
     CDS             29025..30800
                     /gene="fucI"
                     /locus_tag="OVW45_000628"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCV5525153.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALTWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGSVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            30909..32327
                     /gene="fucK"
                     /locus_tag="OVW45_000629"
     CDS             30909..32327
                     /gene="fucK"
                     /locus_tag="OVW45_000629"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HCV5525154.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYADSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            32329..32751
                     /gene="fucU"
                     /locus_tag="OVW45_000630"
     CDS             32329..32751
                     /gene="fucU"
                     /locus_tag="OVW45_000630"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCV5525155.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            32809..33540
                     /gene="fucR"
                     /locus_tag="OVW45_000631"
     CDS             32809..33540
                     /gene="fucR"
                     /locus_tag="OVW45_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCV5525156.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(33584..34684)
                     /gene="rlmM"
                     /locus_tag="OVW45_000632"
     CDS             complement(33584..34684)
                     /gene="rlmM"
                     /locus_tag="OVW45_000632"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCV5525157.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVDMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(34677..35072)
                     /locus_tag="OVW45_000633"
     CDS             complement(34677..35072)
                     /locus_tag="OVW45_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCV5525158.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(35091..36008)
                     /gene="gcvA"
                     /locus_tag="OVW45_000634"
     CDS             complement(35091..36008)
                     /gene="gcvA"
                     /locus_tag="OVW45_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCV5525159.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(36359..36586)
                     /locus_tag="OVW45_000635"
     CDS             complement(36359..36586)
                     /locus_tag="OVW45_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCV5525160.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            36778..37983
                     /gene="csdA"
                     /locus_tag="OVW45_000636"
     CDS             36778..37983
                     /gene="csdA"
                     /locus_tag="OVW45_000636"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCV5525161.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            37983..38426
                     /gene="csdE"
                     /locus_tag="OVW45_000637"
     CDS             37983..38426
                     /gene="csdE"
                     /locus_tag="OVW45_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCV5525162.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(38477..39283)
                     /gene="tcdA"
                     /locus_tag="OVW45_000638"
     CDS             complement(38477..39283)
                     /gene="tcdA"
                     /locus_tag="OVW45_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCV5525163.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(39360..40457)
                     /gene="mltA"
                     /locus_tag="OVW45_000639"
     CDS             complement(39360..40457)
                     /gene="mltA"
                     /locus_tag="OVW45_000639"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCV5525164.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            40666..40742
                     /locus_tag="OVW45_000640"
     tRNA            40666..40742
                     /locus_tag="OVW45_000640"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40700..40702,aa:Met,seq:cat)
     gene            40776..40852
                     /locus_tag="OVW45_000641"
     tRNA            40776..40852
                     /locus_tag="OVW45_000641"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40810..40812,aa:Met,seq:cat)
     gene            complement(40927..42180)
                     /gene="amiC"
                     /locus_tag="OVW45_000642"
     CDS             complement(40927..42180)
                     /gene="amiC"
                     /locus_tag="OVW45_000642"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCV5525165.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            42412..43743
                     /gene="argA"
                     /locus_tag="OVW45_000643"
     CDS             42412..43743
                     /gene="argA"
                     /locus_tag="OVW45_000643"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCV5525166.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IRRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQLDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(43805..45631)
                     /gene="recD"
                     /locus_tag="OVW45_000644"
     CDS             complement(43805..45631)
                     /gene="recD"
                     /locus_tag="OVW45_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCV5525167.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHSHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPIVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRG"
     gene            complement(45631..49173)
                     /gene="recB"
                     /locus_tag="OVW45_000645"
     CDS             complement(45631..49173)
                     /gene="recB"
                     /locus_tag="OVW45_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCV5525168.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYEHLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(49166..52054)
                     /gene="ptrA"
                     /locus_tag="OVW45_000646"
     CDS             complement(49166..52054)
                     /gene="ptrA"
                     /locus_tag="OVW45_000646"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCV5525169.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(52230..55598)
                     /gene="recC"
                     /locus_tag="OVW45_000647"
     CDS             complement(52230..55598)
                     /gene="recC"
                     /locus_tag="OVW45_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCV5525170.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFSIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIRAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLSAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(55611..55934)
                     /locus_tag="OVW45_000648"
     CDS             complement(55611..55934)
                     /locus_tag="OVW45_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCV5525171.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(55919..56326)
                     /locus_tag="OVW45_000649"
     CDS             complement(55919..56326)
                     /locus_tag="OVW45_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCV5525172.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(56323..56886)
                     /locus_tag="OVW45_000650"
     CDS             complement(56323..56886)
                     /locus_tag="OVW45_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCV5525173.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(56877..57347)
                     /locus_tag="OVW45_000651"
     CDS             complement(56877..57347)
                     /locus_tag="OVW45_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCV5525174.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(57532..58326)
                     /gene="thyA"
                     /locus_tag="OVW45_000652"
     CDS             complement(57532..58326)
                     /gene="thyA"
                     /locus_tag="OVW45_000652"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCV5525175.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(58333..59208)
                     /gene="lgt"
                     /locus_tag="OVW45_000653"
     CDS             complement(58333..59208)
                     /gene="lgt"
                     /locus_tag="OVW45_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCV5525176.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(59359..61605)
                     /gene="ptsP"
                     /locus_tag="OVW45_000654"
     CDS             complement(59359..61605)
                     /gene="ptsP"
                     /locus_tag="OVW45_000654"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCV5525177.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(61618..62148)
                     /gene="rppH"
                     /locus_tag="OVW45_000655"
     CDS             complement(61618..62148)
                     /gene="rppH"
                     /locus_tag="OVW45_000655"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCV5525178.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNTSAYRRKRG"
     gene            62833..63522
                     /gene="mutH"
                     /locus_tag="OVW45_000656"
     CDS             62833..63522
                     /gene="mutH"
                     /locus_tag="OVW45_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCV5525179.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLTQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            63591..64304
                     /locus_tag="OVW45_000657"
     CDS             63591..64304
                     /locus_tag="OVW45_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCV5525180.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            64442..64660
                     /gene="ygdR"
                     /locus_tag="OVW45_000658"
     CDS             64442..64660
                     /gene="ygdR"
                     /locus_tag="OVW45_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCV5525181.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
BASE COUNT        15674 a        17392 c        15852 g        15759 t
ORIGIN      
        1 gaggctggca tttttaaatc agataaagtc agtcttatgc ctggcctttg atctctttac
       61 gaccgttgta cggtgctttt tcgcccagag cttcttcgat acgaatcagc tggttgtatt
      121 tagcaacacg gtcagaacgg ctcatagaac cagttttgat ctggcctgca gcagtaccaa
      181 cagccaggtc agcgatggta gcgtcttcag tttcgccaga acggtgagag ataactgcag
      241 tgtagccagc atctttcgcc atcttgattg cagccagagt ttcggtcaga gaaccgatct
      301 ggttgaattt gatcaggatg gagttagcga tacctttttc gataccttct ttcaggatct
      361 tggtgttggt tacgaacagg tcgtcaccaa ccagctggat tttgtcgccc agaactttgg
      421 tctggtatgc gaaaccgtcc cagtcagatt cgtccagacc gtcttcgata gatacgatcg
      481 gatactgttt ggtcagttct tccaggaagt gagtgaattc ttcagaggtg aaagctttgt
      541 tgccttcgcc agccagaacg tatttaccgt ctttgtagaa ttcagaagct gcgcagtcca
      601 tcgccagagt gatgtctttg cccagttcat aaccagcagc tttaacagct tcagcgataa
      661 cagccagagc ttcagcgttg gaacccaggt tcggcgcata gccaccttcg tcaccaactg
      721 cagtgttcat gcctttcgct ttcagaactt ttgccaggtg atggaaaact tcagaaccca
      781 tgcggatggc ttctttcaca gttttcgcgc caaccggctg aatcatgaat tcctggatat
      841 caacgttgtt gtcagcgtgc tcaccaccgt tgatgatgtt catcatcgga accggcatgg
      901 agtatttgcc cggagtgccg ttcagttcag cgatgtgctc gtacagcggc atccctttag
      961 cggcagcagc agctttggcg ttagccagag atacagccag gattgcgttc gcgccgaatt
     1021 tggatttgtt ttcggtgccg tccaggtcga tcatgatctt gtcaatgcca gcctgatctt
     1081 tagcgtcttt gccaatcagt gcctgagcga tcgggccgtt taccgcagca acagctttgg
     1141 ttacgccttt acccaggaaa cgggatttgt cgccatcgcg cagttccaga gcttcacggg
     1201 aaccagtaga agcacctgac ggagcagctg ccataccgac gaaaccaccc tccagatgta
     1261 cttcggcttc aacagtcggg ttaccacggg agtcgatgat ttcacgaccg atgattttta
     1321 cgattttgga cattaggttt tcctcaagtc actagttaaa ctgaaactcc agacaaacaa
     1381 cgcgtaccca aggtacgcgt tgccgttcta acttttttac ttacttcgcc tgacgtttct
     1441 ggaactcgct ggcagctttc acaaagcctg caaacagcgg gtgaccatca cgtggagtag
     1501 aagtaaactc cggatggaac tggcaagcca cgaaccacgg gtgattcgga acttcgatga
     1561 tctcgaccaa ctgatcatcc ccggaacggc ccgcaacgcg cagacctgca tcttcaatct
     1621 gtttcaacag catgttgttg acttcgtaac ggtgacgatg acgctcaaca attgtcggcg
     1681 cattgtacag ctggcgaacc aggctatcgt caaccaactg gcactgctgt gcgccgagac
     1741 gcatggtacc gccgagatcg ctcttctcgc tacgaacttc aacgttgccg ttttcatcgc
     1801 gccactcggt aatcagcgcc acaaccgggt acttacagtc tggcacaaat tccgtagagt
     1861 tggcgttctc catgttggca acatggcgag cgtaatcaat taacgccacc tgcataccca
     1921 ggcaaatgcc cagataagga atattgttct cacgcgcaaa acgcgcggtc gtaatcatgc
     1981 cttctacgcc acgatagccg aaaccgccag gtacgaggat tgcgtccaga cctttaagga
     2041 tttcaacgcc gcgcgtttca acatcttgtg aatcgatcag tttgatgttg acgctgacac
     2101 gattcttcag cccaccgtgt ttcagtgctt cgatcactga tttataagca tccggcagtt
     2161 caatgtactt gccgaccata ccgatggtga cttcacttac cggattcgct tcttcgaaga
     2221 taacctgttc ccattcggac agatttgctt ccgggcagtt taagctgaat cgtttacaaa
     2281 tataatcgtc cagcccctga gatttcaaca ggcccggaat tttatagatg gaatcgacgt
     2341 ctttcagaga aataaccgct ttttccggaa cattacagaa caatgcaatc ttcgcacgtt
     2401 cgttcgccgg aacagcgcga tctgaacgac aaatcaggat gtcaggctgg ataccgatgg
     2461 agagcagctc ttttacagag tgctgagtcg gtttggtttt gacttcacca gacgctgcca
     2521 tgtacggcac cagcgtcagg tgcataaaca gagtgtgctc acggccaatt tcaacagcca
     2581 tctggcgaat cgcttcgagg aacggcaagg attcgatatc acctactgta ccgccgattt
     2641 ctaccagtac tacgtcatga ccttcgccac cttccagcac gcgctctttg attgcgttag
     2701 tgatgtgcgg aataacctgc acggttgcgc cgaggtagtc accgcggcgt tctttacgca
     2761 gaacgtcaga gtagatacga cccgtggtga agttgttgcg gcggctcatt ttggtacgaa
     2821 tgaaacgctc gtagtgcccc aggtccaggt cggtttcagc gccgtcttca gtaacgaaca
     2881 cttccccgtg ttggattggg ctcatagtac ctggatcgac gttgatgtac ggatccagtt
     2941 tcatgatggt cacattgagg ccacgggctt caagaatggc tgcgagggag gctgcggcaa
     3001 tgcctttacc cagagaggat acgaccccgc cggtcacaaa aatatagttc gttgtcatgc
     3061 tgaacctgag aagttaggtt gaaaagacga tggaataacc aggacgggaa agcagtatac
     3121 ccgaacatga cctgtgccac aaactttcat tatccctcct cttcgccagc gcactattga
     3181 aatcaggagt gagaaaatag cccctttggg gtaaatgttt ttgacgcaaa tcaagcgctt
     3241 gtcatttaaa aaatgacaca aatggcgctt gaccgcgtaa ttcccttaga gatcaatttc
     3301 ctgccgtttt acctgttgcc agacttcttc cattgtttcg aggtcaacac ctgtcatttc
     3361 cagtccacgc gcggcaacaa tacgctccac ttcgcgaaaa cgacgctcga atttttcgtt
     3421 cgctttttgc aatgcgattt ctgctttcgt ccctaaatgg cgagccagat taaccgtggc
     3481 aaacagcagg tcccccattt cctcctccag tttagcctgg tcgacaacag cctgccgcgc
     3541 ctcgtacatc acctcgtcga tctcttcgta gactttatca accaccgggc caagcgtcgt
     3601 ccagtcgaag ccaacgttgg cgcaacgttt ctggattttt tgcgcccgca ttaaagccgg
     3661 taaactacga ggaatatcgt ccagcgccga atgctgcgct ttctgcgcgc gctcttcggt
     3721 tttgatttgc tcccaacggg caagcacttc actactgttt tcggcagaac tatcagcaaa
     3781 aacatgcgga tggcgacgct ctaatttatc gctaatagca gcgcaaatat cattaaagtc
     3841 aaagcgccct tcttcctgag ccatttgcgc gtaaaacacc acctggaata gcagatcgcc
     3901 cagttcaccg cgaagatcgt caaaatcttc acgggcgatg gcgtccagca cttcgtaggt
     3961 ttcttcaagg gtgtaaggcg caatggtggc aaatgtctgc tctttatccc acgggcagcc
     4021 gttttccgga tcgcgcaggc gctgcataat agtgagcaaa cggtcgattt gattcattga
     4081 attgtcctga aaattgcggg tctgtcaggt ggaaacctgt gaccagaata gaagtgagtt
     4141 agtaacacta cccaatcagt acgttaattt tggctttaat gagttgtaat tcctctgggg
     4201 caactgttcc tttcttcgtt gctcctcttg cccgccaggc gatacttttt acctgatcag
     4261 ctaacgctac gccatcacgt tcctgaccgg ataaaacaac ttcgaacgga tatccttttg
     4321 attgcgttgt acaaggaaca cacagacaca tacctgtttt gttgttgtac atgaaaggac
     4381 tcaggacaac agctggacga tgtccagctt gctcgctacc ttttgtcggg tcaaaatcaa
     4441 cccaaatcag atcgcccata tcgggtacgt atcggcttac cattaccaga cttccttatc
     4501 tttcggctct ccccagtcga tattctcgtg gaggttttcc ggcgtgatgt cgttgaccag
     4561 ttcagcaagc gtaaatacgg gctctttacg cactggctca ataattaatt tgccatccac
     4621 caggtcaatc ttcacttcat catcaatatt cagattgagc gcctgcatta acgtagccgg
     4681 gatccgcacc gccggtgaat ttccccaacg ctttacgcta ctgtggatca taaccctttc
     4741 ctcaaaccgc tatcatatgt agatacagta tatatcaatc tacattgtag atacgagcaa
     4801 atttctgcct aactcccgtg caaccgacgc gcgtcgataa catccggcac ctggttgagt
     4861 ttacccagca cgcggcccag cacctgcagg ttgtaaatct caatggtcat gtcgatggtt
     4921 gccagttgct gttttgtgtc gctacggctg gcaacgccaa gcacgttcac cttctcgttg
     4981 gcgagaatgg tcgtgatatc acgtaacaac ccactacgat cattagccac cacgcggacc
     5041 accagcgaat atccggcgga gtagctctca ccccataccg cgtcaacaat gcgttctggc
     5101 gcatgagagc gcagttccgc cagttgttcg caatcggcgc ggtgtactga aataccgcgc
     5161 ccctgggtaa tgaagccgac aatctcatct ccaggaatcg gctggcagca gcgcgcgatg
     5221 tggtgcatca ggttgccaac accttcgact accacgcgac cgttatcttt actgcggttt
     5281 tgcggcgtgt agcttttttg ctgaagttgc ttcagcgcgg cggcgtcctg ctcttcggca
     5341 ctcggcttat taaattgcga ttgcaggaag ttcaccatct gattgagacg gatatccccg
     5401 ccaccaatcg ccgccagcaa ctcgtcgaca tcattaaagt tgtaacgcgg cagcagatgt
     5461 ttttctgctt ctttcaggct gatccccaga tgttccagct cgtcgtcaag gatttgccgc
     5521 ccagccagaa tgtttttgtc acggtcctgt ttacggaacc aagcgtgaat tttcgaacgc
     5581 ccacggctgg ttgtgacgta accgaggttt gggtttaacc agtcacggct ggggttcggc
     5641 tgtttctggg tgataatttc aatctggtcg cccatctgca gctggtaggt gaacggcaca
     5701 atgcgcccgc caatttttgc cccgatgcag cggtgtccga catcactgtg gatgtggtaa
     5761 gcgaagtcca gcggcgttga tcccgcaggc aaatcaacga catcaccttt cggcgtaaag
     5821 acatacaccc ggtcgtcaaa gacctggctg cgtacttcgt cgagcatttc gccggaatca
     5881 gccatctctt cctgccacgc aatcagttta cgcagccagg caatccggtc ttcatgtccc
     5941 gaacgtgcgc cgccagctgc cgcgccctct ttatatttcc agtgcgcagc aacacccaac
     6001 tctgcatctt catgcatctg tttggtgcgg atttggatct caacggtttt gccacctggc
     6061 cccagaacca cggtatgaat agactgataa ccgtttggtt tcgggttagc gacataatcg
     6121 tcaaactcat ccggcaggtg gcgatagtga gtgtgcacta tccccagtgc ggcatagcaa
     6181 tcctgtaaac gctcggcgac aatacgtacc gcacgcacat caaacagctc atcaaaggcg
     6241 aggttctttt tctgcatttt acgccagatg ctgtagatgt gtttcggacg accatacact
     6301 tccgctttaa cgccttcagc tttcatctca gcgcgcagat gaccaacgaa ctcttcgatg
     6361 tagtgttcgc ggtcgagacg ccgttcatgc agcagtttgg caattcgttt gtattcggtt
     6421 ggatggaggt aacggaagca gtaatcttcc agttcccatt tcagttgtcc gattccgaga
     6481 cggttagcca gcggtgcgta gatgttggta cactcttttg ccgccagtac acgttcatct
     6541 tccggcgcat cttttacttc gcgcagatga gcaatacgct ccgccagttt gatgactacg
     6601 cagcgaaaat catcgaccat cgccaataac atccggcgaa cgttatcgac ctgttcggag
     6661 gaaacagaat cagtgtgcgt cgctttcagc tggcggatcg ccgccatatc acgcacgccg
     6721 tgaataaggt taacaaccga cttaccgacg ctctcacgca gcacatcttc gctgactacg
     6781 ttggcatcag ccagagggaa cagcagcgcc gcccgcagcg tgtcaatgtc catacttaat
     6841 gtcgagagga tctccaccat ctcaacacca cgccacaata acagactggc atccggatgc
     6901 ccctgcgtct gttgcagaca atacgcccaa gtttcggcta agcactcaca cgacttctgg
     6961 ctggtaatac ccagacttgc gatccatttt tccggatcaa attcaccagc cttattgata
     7021 tgtgcacttc ttaccgcaac catcgtcctc tcctttaggg accagacctg ccgaaatcgg
     7081 caaatcgcaa ctatttaacg cgcgagaaaa gtaccatcga ttccagatgt cccgtgtgtg
     7141 ggaacatatc cagcatcgcc agtcgcgcaa tggtatatcc tgcttttaat aacgcttcgc
     7201 tatcccgagc cagcgttgca gggttacagg atacataaac tatacgaata ggttccagtt
     7261 ttataatttg ctgcataaca cctgcggcac ctgctcgcgc cgggtccagc aacactttat
     7321 cgaagccgtt tttcgcccac ggctgctttg tgacatcttc ttcaagattt tcgtgataaa
     7381 acgtcacatt ctgtaagcca ttaagacgcg cattctgctg gcctttttcc accagcgccg
     7441 gaacgccttc tacacccacc acactggcag cttgtgtcgc caatggcagt gtaaagttgc
     7501 ccataccgca gaacaaatcc agtacgcgat cttcaggttg cacatccagc cattccaacg
     7561 cacgcgctac cattttttgg ttcacacccg cattgacctg aataaaatcg cgcgggctaa
     7621 aagttaagcg caacccattt gagtcatacc agggcatctc accagagacg gtttcgagta
     7681 tctcactatc gggggcgaga tacagatcca ggccttcaga atgcgaaaag cgttccagtt
     7741 tttcgcgatc tgccgaactt agcggtgcgg tatggcgcaa aatcatcagc gtgccgctgg
     7801 ttgcctgtac cagttcaaca tgaccaagat ggcgcataac ttgtaagctg cccaggcatg
     7861 ccatgacttt gggcagcaat gcttcaagtt ggggcactaa aatggggcat tgtttaacgt
     7921 cgacaatgtc actggagccc gctttgcgaa accccatctg aagttgctgt gttttcggta
     7981 agtagttcag acttaaacgc gcgcgacggc gatagcccca gggaacatcg gcgatcactt
     8041 cagagacttc gtgtttcatt aatcgggcga gtgccgcact tttgcttcgc tgctgtaaat
     8101 ccacgctggc gtgttgttgc tgacagccgc cgcatacgcc aaaatgagga cagcgtggcg
     8161 tttcgcgtcc cgggctatcg cttaaccggc gtacgacttt agcgcgggca tactgttttt
     8221 tatcttcagt aacagtaact tccgcgtttt cctgcggcaa taatccgggg ataaatagcg
     8281 ttttgccgtt atgtcgcgcc acgccctgac caaaagagtc gaggtcgttg actgaaacgg
     8341 ttatgatctg acgcgtcgtc gtgcgtcgtt ttgcagagta gaattgcgcc attggcgaga
     8401 ctttctcaat ttaacagtgt gaccttaatt gtcccataac ggaactccat gaccaactac
     8461 agcctgcgcg cacgcatgat gattctgatc ctggcaccga ccgtccttat tggtttattg
     8521 ctgagtatct ttttcgttgt gcatcgctat aacgacttgc agcgtcaact ggaagatgcc
     8581 ggagccagca ttattgagcc gcttgcagtt tctactgaat atggcatgag cctgcaaaat
     8641 cgcgaatcta tcggtcagtt aataagcgta ctgcatcgtc gccattccga tattgttcgc
     8701 gcgatttcgg tttatgatga aaataaccga ctctttgtca cctccaattt tcatcttgat
     8761 ccctcatcaa tgcagctcgg cagcaacgtg ccgtttcctc gccagctcac tgtcactcgt
     8821 gacggcgata ttatgatcct ccgcacgccg attatttctg agagttactc ccccgacgaa
     8881 tcgcccagta gcgatgccaa aaatagtcaa aatatgttgg gatatattgc gctggagctg
     8941 gatcttaaat cggttcgctt gcagcaatat aaagagatct ttatttccag cgtgatgatg
     9001 ctgttttgta tcggtattgc gcttattttt ggctggcgct taatgcgcga tgtaaccggt
     9061 ccgattcgca acatggtgaa taccgtcgac cgcatccgtc gcgggcaact cgacagccgg
     9121 gtggaaggat ttatgctcgg cgagctggat atgctgaaaa acggtatcaa ctcgatggca
     9181 atgtcgctgg ctgcttatca cgaagagatg cagcacaata tcgaccaggc gacgtccgat
     9241 ctgcgtgaaa cgctggagca gatggaaatt cagaacgttg agttagatct ggcgaaaaag
     9301 cgcgcccagg aagcggcgcg tattaaatcc gagtttctgg caaatatgtc acacgagctg
     9361 cgtacaccac tgaatggtgt tattggcttt acccgcctga cgctgaaaac agaattaaca
     9421 ccaacgcagc gcgatcacct gaatacgatt gaacgttcgg caaataattt gctggcaatt
     9481 attaatgatg ttctcgactt ctcgaaactg gaagcaggta agctgattct ggaaagtatt
     9541 ccattcccac tacgcagcac gctggatgaa gtcgttactc tgctggcaca ttcttctcac
     9601 gataaagggt tggaactgac gctcaatatt aaaagcgacg tgcctgataa cgtgatcggc
     9661 gacccactgc gattacagca aatcatcact aacctggtgg ggaatgcaat taaattcacc
     9721 gagaatggca acattgatat tctggtagaa aaacgtgcgc tgagtaatac caaagtgcag
     9781 attgaagtgc agattcggga taccggcatt ggtattcctg aacgcgatca atcgcgctta
     9841 ttccaggcct tccgacaggc tgatgccagt atttcccgcc gtcatggtgg caccggtctg
     9901 gggctggtga ttacacaaaa actggttaat gaaatgggcg gcgatatttc gttccatagc
     9961 cagccgaatc gcggttcaac tttctggttc cacattaatc tcgatctgaa cccgaacatt
    10021 attatcgaag ggccatccac ccagtgcctc gccggtaagc gcctggccta tgtcgaacca
    10081 aactccgcag cagcgcaatg cacgctggat attttaagtg aaacgccgct ggaagtggtt
    10141 tatagcccaa cgttctccgc gctgcctccc gcgcattacg acatgatgtt gttaggcatc
    10201 gcggtgacct tccgcgagcc gctaacaatg caacatgagc gattagcgaa agcggtatcg
    10261 atgaccgatt tcctgatgct ggcacttcct tgccatgcac aagtcaatgc agaaaaactc
    10321 aagcaagatg gtatcggcgc gtgtctgctg aaaccattaa cacctacgcg cctgttgcct
    10381 gccctgacgg aattttgtca tcacaaacaa aacacgcttt tgcctgtaac cgatgaaagc
    10441 aagctggcaa tgacagtcat ggcagttgat gacaaccccg ctaacctgaa acttatcggc
    10501 gcattgctgg aagatatggt gcaacatgtg gaactttgcg atagcgggca tcaggcggtt
    10561 gaacgggcga aacagatgcc gttcgatttg atcttaatgg atattcaaat gcctgacatg
    10621 gatggcattc gggcctgcga gctcatccac cagctccccc atcagcaaca aacgccggtt
    10681 atcgcggtaa cggcgcatgc aatggccggg caaaaagaga agctgcttgg cgcagggatg
    10741 agcgattatc tggcgaaacc gattgaagaa gagcgattgc ataatttgtt gttgcgctac
    10801 aagcctggca gcggtatttc ctctcgcgtc gtgacgcccg aagtcaacga aattgtggtg
    10861 aacccgaatg cgaccctcga ctggcaactg gcactacgcc aggcagcagg aaaaaccgat
    10921 ctagcgcgcg atatgctgca aatgttactc gatttcctgc ctgaagttcg caacaaagtt
    10981 gaggagcagc tggttggaga aaacccggaa ggcctggttg atttgattca taaactgcat
    11041 ggcagttgcg gctatagcgg tgtgccgcgt atgaagaatc tctgccaact gatcgaacaa
    11101 cagctacgta gcggtactaa agaagaagat ttggagccgg agctgctgga actgttggac
    11161 gagatggata atgtcgcgcg cgaagccagc aaaattctcg ggtaacggac gtcttgtcga
    11221 gtagcaatcg gctttttatg acgctggttt cagattggtg acaaagtgcc tgtgtttatg
    11281 ccggatgcgg cgttaacgcc ttatccggcc tacaaaacca attaaattca atgaattaaa
    11341 aaatgatgta gacctgatac atagcacaac aggctatttt gcgtttagca tcagtctcaa
    11401 gccggctcca gatagagccg gttttggttt tctgtcttaa cgcaccatgc acgggcgctt
    11461 gttatcgaac gtccagccag gaatcagata ctgcattccc atcgcatcgt cacgcgcgcc
    11521 aagcccgtgt ttctgataca gctcatgggc tttcatcact tgatccatat cgatttctac
    11581 acccagcccc ggtttttccg gcacctgaac cagaccgcct ttgatctcaa acggttcttt
    11641 ggtcaagcgc tgattgcctt cctgccaaat ccagtgcgta tcaatcgcgg tgattttacc
    11701 cggtgcagcg gcggcaacat gggtaaacat cgccagggaa atatcgaagt ggttgtttga
    11761 gtgtgaaccc caggtcaggc caaattcatg gcacatttgc gccacacgta ccgaaccttg
    11821 catagtccag aaatgcggat ccgccagcgg gatatcaacg gattgtaacg agagcgtatg
    11881 gcccatctgc cgccagtcgg tggcgatcat attggttgca gtcggcaggc cagtcgcgcg
    11941 acggaactct gccatcactt cacgcccgga gaaaccttgc tccgcaccat acggatcttc
    12001 tgcataagcc agcgaacctt tcaggtattt accaatttta attgcttcgt tcagcgacca
    12061 ggcaccgtta ggatcgagcg taatacgcgc ctgcgggaag cgttgcgcca gtgccacaat
    12121 agactcggct tcttcttccc cggccagtac accgcctttc agtttgaaat cgttgaagcc
    12181 atatttttca tacgccgctt ccgctaggcg caccaccgca tccggcgtca tcgcttcttc
    12241 atgacgcagg cgataccagt cgcatgagtc atccggctgg ctttgatacg gcagaggtgt
    12301 ggctttgcga ttaccgacga agaacagata accgagcatt tcgacttcgc tacgctgttg
    12361 accatcgccc agcagcgatg ccacgtttac ccccagatgc tgccccagca gatccagcat
    12421 tgccgcttct atcccggtaa ctacatgaat agtggtacgc aaatcaaatg tctgcaaacc
    12481 gcgcccacca gcatcacgat cggcaaaagt attacgcacc agcgtcagaa cgtttttgta
    12541 ttcacccagc gttttaccta ccaccagcgg aatcgcatct tccagcgttt tacggatttt
    12601 ctcgccgccg ggaatttccc ctacgccagt gtgaccggaa ttatctttga taatcacaat
    12661 attacgcgta aagaacggtg cgtgtgcacc actcagattc atcagcatac tgtcatgacc
    12721 cgccaccggg atgacctgca tttcagtaac aacagacgtc gtaaattgag aacttatatt
    12781 tatgtccttt ttcagaatca atgacggccg aaaacgggac gtttacggtc aaaggtccag
    12841 ccggggatca ggtactgcat cggacctgcg tcgttacgcg caccgccagg cagacgttta
    12901 taggcctcat gtgccttttg tacctgttcc cagtccagtt ccacgcccag accgggcgca
    12961 tcaggaacgg caatttttcc gtttttaatc tccagcggat ttttggtcag gcgacaatcg
    13021 ccctcctgcc aaatccagtg ggtatcgata gcggtaggat tacccggtgc cgccgcgccc
    13081 acatgggtaa acatcgccag agagatatcg aaatggttat tagaatggca gccccaggtc
    13141 agcccccagt cgtcgcaaag ctgcgccaca cggactgcac cggaaagcgt ccagaagtgc
    13201 ggatcggcaa gtggaatatc taccgcattg agcatcaccg catgacccat ttcgcgccag
    13261 ttggtggcga tcatgttagt cgcgacgggc aagccggtcg cccgccgaaa ctccgccata
    13321 acttcacgac cggagaaacc ctgttctgcg ccgcatggat cttcggcata ggtaagaaca
    13381 tcattcagcc ctttgcatag agaaatggct tcatcaagta gccaggcacc gttgggatca
    13441 acggtaatcc gcgcatcagg gaagcgtttc ttcaatgcac gaacagtgtc gatttcttgc
    13501 tcgccaggta acaccccgcc cttaagtttg aaatctttaa agccatagcg atcctgtgag
    13561 gcttccgcca gacgcacaac ggcttcgctg ttcatcgctt tctgatggcg caactgatac
    13621 cactcatggt tgcccggcgt attttccaga taaggaagat cggttttggt ccgatcaccg
    13681 atataaaaca gataaccgag gacggtaata gtctcgcgtt gcttgcctgg ccccaacagt
    13741 tcgcagaccg gaacattcag cgccttacct agcaggtcaa gcaaggcggc ttccagcgcc
    13801 gccacggcat taacgcgcaa ttcaaaagtc caggcacctt tgccgaaggt atcaaaatcg
    13861 gctgcctgat tacctttatg cacctgctga accactttat tcaggcgcgc aacttcctgg
    13921 cccagaacca tcggaatagc atcgaccagt gtttgataaa tcacctctcc gcccggcgct
    13981 tcaccaatgc cggtatgccc ggcgttatcg gtgagtacca caatattgcg ggtgaaatat
    14041 gcgttatgtg ccccaccaat attaagcaac atgctatcgt gcccggcgac cggaatgact
    14101 ttcatatcag taataacagg actggattgt gtcgtcatct tattgccccg cgacaggttt
    14161 cagctcgata cgcttgatat cgcccaccag caccaggtag ctcagtaccg cgattaaggc
    14221 atgaacacca acataaatca gcgccccatt aaaggagcca gtcgtgccaa cgatataacc
    14281 aattgcgatt ggcgtgacga tgccagaaat gttaccgaac atgttgaaca ggccaccgga
    14341 aagaccgctg atctcttttg gcgcggtatc tgccattact gcccaaccca gcgccccgat
    14401 gcccttaccg aagaaggcca gcgccataaa gccgatgatc atccactcaa cgttgacgta
    14461 gttgcagaac accatcacca tcgacagcaa catgcccatt acgatcggtg ttttacgcgc
    14521 aatgttcagc gatcccgtgc gacgcatcag ccagtcggaa ataatcccac ccagcacacc
    14581 gccgataaaa ccgcaaaccg ccggaacgga agccacaaag cccgctttca gaatcgacat
    14641 cccacgcgcc tgcaccagat aaaccggaaa ccaggtaatg aaaaagtaag tcagggcgtt
    14701 gatacagtac tgaccgatat aaacgccgat catcatccgt gacccgagca gttgtttgat
    14761 ctgcccccac ttcacgctga acggcacttt aactttggtg ttttgctgat ccatattgat
    14821 cagcgcgcca cccgcggcga tgtactccag ctctttctga tttacccccg gatgttgatt
    14881 tggctcgtgg atgactttca accagataaa gctgatgaca atccccagac cgcccataaa
    14941 gaagaagacg tgtgaccagc ccacttcatg cgtcagccag cccataatcg gcgcgaagat
    15001 caccgttgcg aagtattgag cggagttaaa aatcgacacc gccgttcccc tttcctgcgc
    15061 cggaaaccag gccgcaacaa tgcgactgtt gccggggaaa gatggcgctt cagcaagccc
    15121 gaccaggaag cgcagagtaa acagggcaac gataatgccg aatccactaa agatatcgac
    15181 gaagccttgc agcaaggtaa acatcgacca gataaagatc gaccagaagt agacgcgttt
    15241 tgaaccaaaa cgatccagca accaaccacc agggatctgc ccgataacat aagcccatga
    15301 gaaggcagag aacacatagc ccattcccac gggatcaagg ccgatatctt tggccatttc
    15361 cgaaccggca atagaaagcg tagcgcggtc gccgtagttg aaggatgtga cgataaacaa
    15421 catcaccact atccagtaac gagcattcgt gcgtttttcc acactgctcg cagcctgact
    15481 taaagaactc attgttgcac tcctgaaaat tcgcgttagc cacgctcact ctggactgcg
    15541 acatagccag gaaatcagag gtgacgtagg gtgttttttg ccgtttttat aggtcgttcg
    15601 ccgaatacgg cgcgtgttta tatctggcaa tagcagtata aaaagcgcgc catagcggct
    15661 caccgtgcaa caacacaaca ttaatgcgtt caatgaggcc cgattttggc attagccctg
    15721 gacggtggaa tccacttcac ggaaatgaaa acaagaacaa gaaaggaagg gttaaaacga
    15781 agaaataaag aagagtatga aatggatcgc ttgactccag gcaaacgcca gtaaaaatcc
    15841 gcgctatgaa gcagttttta ctggcatttg cctgaaaaga ttcgattcag caccgctaaa
    15901 acgacattta ccgctcgctg aacatatcag gacaacagcg tgccccactg ttcgacccac
    15961 ggatttgatt ccgtttccgg ttccgggttc tcactggcgt caatcaacag catttcgcca
    16021 acccgctgag cgctctgttc ctgcaacaag gcatcgaact gtttgccgcc attgcagaaa
    16081 tttacataac tgctgtcgcc gagcgcaatc acgccataac gcagattcgg ctggaagccc
    16141 agactatctt tgattccctg aaagagcggc acaatgctat caggaaggtc gccctgcccg
    16201 gtcgtggacg taaccaccag aacatactta tcctgataag gcagccagtc gcttaattca
    16261 ggatcttcaa ataccgttgc tttgtggccc tgtgcggtca gaattgcttc tgcttcttcc
    16321 gccactaaca gcgaatttcc gtacatggtg ccgacaaaaa taccaatttc cgccataccg
    16381 ttttctccct ggattaggaa cttatctctc catcctgacc cgatggcgca ctgaactcaa
    16441 ccctttcatt ttcaggaagc agaccgcgcc agccaaattg tgataacgcc tgcatccagg
    16501 tgtcgtccag gcccgcgtga atagtcagcg gctcgccagt aaaaggatgc gtcaatgaca
    16561 actgactggc atgtagcatt aaccgctgga ggccaaaatg ctcagcaccg ctgcgattct
    16621 ggcgtaaatc gccatgtttg ctatcgccaa taatcggatg acgcaaatgg gcaagatgtc
    16681 ggcgaagctg atgtttgcgt ccggttttcg gctccagttc caccaggccg tagcgcgtgg
    16741 tcgggtaacg tccggtcgct accggcattt ctacggtcgc cagaccgcga taatgcgtca
    16801 ctgctggctg cgggccttta tcttcgcggg caaatttatc agcgattttg tccagttctt
    16861 ccaccagtgg ataatccagc accgcttctt ccatcaacca gccgcgcaca atcgcatggt
    16921 aacgtttctg gatttggtgc tgttcaaact gttgtgccag cagccgtccg gcctcgctgg
    16981 ataatcccat caacaacaca ccagaagtgg gtcggtccag acgatgagca gtaaaaacat
    17041 gctgtcctat ctggtcacgc acggtttgca tgaccactac tttctcgtcg cgatccagcc
    17101 agctacggtg aaccagccag ccggagggtt tatttaccgc aaccagccat tcatcctgat
    17161 agagtatttc cagcattagc tcgcatcatc cgcaaaaaga gcatccagtt tttccaactc
    17221 agccagaata agcgcgcgtt gcggatggtc cgtcgccagc gccatttcat aatagggtgc
    17281 aacagcaaaa gcgcccggta acggctggtt gttatttaac aaatcgtgca ttcgcgggat
    17341 cagcacccac tgcaaccact ccagtggttc catggtgtcc ataaagaacg gttgggtact
    17401 attaaattga tgcggctggg gttcatcgtt tcgccagtgc tgatgttcac gcagtaacgc
    17461 ttcaagcgtc tgcaactgga gacgaacgcg gtcatgagtg gtcataaagc aacttcaata
    17521 agaaaaactg ccgcgaagga tagcaggaat aaaaaaaggg agcactgtat tcacagtgct
    17581 cccggttcgt ttcgcagcat tccagctact tttgttgctc cctgctcatc cttgacaact
    17641 tttcctctgg ccttgcggcc aatcgttcat cctgaactat tgcttcctgc tcacaccacc
    17701 ccgatgtgat acttcatcct gaagtgtccc tggccttcct gacccaccga atcatcctga
    17761 ccggttctca ttctccatcc tggaggtgtc ctttaacgcg tcctgcgtca tcctcttcgc
    17821 ttcatccaga agcctttccc tgaaacacca tcctggtgtg tcctgcagaa gtgtcatcat
    17881 cctgatgttc acttcgttgt ctgactccct gtcgacgaag atagaatcgt ctttttcgcg
    17941 aagtcttaca aggcgcttac agacaatgcc ttaaggaaaa tttcatatga aagttaagaa
    18001 ttaacaatca acattttgat tttaaatgaa aataacgatt cttatctttt taagtattcg
    18061 cttatttcct ggcgatctcc tacagggtct gtaagagatc tctcacaacc agatggggta
    18121 tttggattac agaagaggct taagttgatt aaggaattcc gcaagatttg aggctaaatg
    18181 cgtccgttta cgtgttccca gcgtctcttt gcacacttca ccggagagat tacacaccga
    18241 gatgacctct aactcttctt ccagcgtcgc gataaatagc gtgggcggca gttttagcct
    18301 tttctgagtc accagatgac caatcagatt ttcctgcacc cgacggaagt cgtcttcact
    18361 ccaggtttgc agcaatgtca gtttgatatc gccaaactgc gcgtgcatat ccccggcaaa
    18421 ctgagtcgta taaaaggtat gaattgtgga ttgtatcaca atatcaaaag cgcgttcaac
    18481 cgcgtttaca ttttgttccc cggtaaacgg ctggggttgc cagtatacgg catcttcggt
    18541 agttgaaata atgcacggtg aaggaacacc gtacagttcc tcacttaacg gccagctttt
    18601 atgctcttca tgccacgcat cgcagtagcg tgcagtaaaa tctttcaggg cttgtgcggt
    18661 caaatcgtcc actaatttct ctcttcactt aaaccagata cacttgtcgt ttagtttatc
    18721 tggtttatga cggtgaaaca tgtcttctta tgcaaaccat caggcacttg cgggcctgac
    18781 tcttggaaaa tcaaccgatt accgggatac ctatgacgcc agcctactgc aaggcgttcc
    18841 acgcagcctg aatcgcgacc cgctgggtct gaaagcggat aacctgcctt ttcacggtac
    18901 ggatatctgg acgctgtatg aactttcctg gctgaatgcg aaaggtttgc cgcaggtcgc
    18961 tgtcggtcat gttgaacttg attacaccag cgtaaatctg attgagtcga agagttttaa
    19021 gctctatctc aacagtttta accagacgcg ttttaataac tgggatgagg tgcgccagac
    19081 gctggagcgc gacttaagca cttgcgctca gggtaagatt agcgtggcgt tatatcgtct
    19141 tgatgaactg gaaggccagc cgataggtca ttttaatggc acttgcattg atgaccagga
    19201 tatcactatc gataactatg aattcactac tgactatctg gagaatgcca cctgtggtga
    19261 aaaagtagtg gaagagacgc ttgtcagcca cctgctgaaa tcaaactgcc tgatcaccca
    19321 tcaaccagat tggggttcga tccaaattca gtatcgtgga cgccaaattg acagagaaaa
    19381 actgctgcgt tacctggtct cattccgtca tcacaacgag ttccacgaac agtgcgtgga
    19441 acgcatcttt aatgacctgt tacgcttctg ccagccagaa aaattgagcg tttacgcacg
    19501 ttatacccgt cgtggcggtc tggacattaa cccgtggcgc agtaatagcg attttgtccc
    19561 atcgaccaca agactggttc ggcaataaat tttttctcaa ttttgcgtgc tggattcacg
    19621 cagaaggttg tgaaaggaca tcaggcaggg ctattgtaat caaagggaat gacgatattc
    19681 gtcccataag gagtttttct tgattacaca tattagcccg cttggctcca tggatatgtt
    19741 gtcgcagctg gaagtggata tgcttaaacg caccgccagc agcgacctct atcaactgtt
    19801 tcgcaactgt tcacttgccg tactgaactc cggtagtttg accgataaca gcaaagaatt
    19861 gctgtctcgt tttgaaaatt tcgatattaa cgtcctgcgc cgtgaacgcg gcgtaaagct
    19921 ggaactgatt aatcccccgg aagaggcttt tgtcgatggg cgaattattc gcgcgttgca
    19981 ggccaacttg ttcgcggttc tgcgtgacat tctcttcgtt tacgggcaaa tccataacac
    20041 cgttcgtttt cccaacctga atctcgacaa ctccgtccac atcactaacc tggtcttttc
    20101 catcttgcgt aacgctcgcg cactgcatgt cggtgaagcg ccaaatatgg tggtctgctg
    20161 gggcggacac tcaattaacg aaaacgagta tttgtatgcc cgtcgcgtcg ggaatcagct
    20221 gggcctgcgt gagctgaata tctgcaccgg ctgtggtccg ggagcgatgg aagcgccgat
    20281 gaaaggtgct gcggtcggac acgcacagca gcgttacaaa gacagtcgtt ttattggtat
    20341 gacagagccg tcgattatcg ccgctgaacc gcctaacccg ctggtcaacg aattgatcat
    20401 catgccagat atcgaaaaac gtctggaagc gtttgtccgt atcgctcacg gcatcattat
    20461 cttccctggc ggtgtgggta cggcagaaga gttgctgtat ttgctgggaa ttttaatgaa
    20521 cccggccaac aaagatcagg ttttaccatt gttcctcacc ggcccgaaag agagcgccga
    20581 ctacttccgc gtactggacg agtttgtcgt gcatacgttg ggtgaaaacg cgcgccgcca
    20641 ttaccgcatc atcattgatg acgccgctga agtcgctcgt cagatgaaaa aatcgatgcc
    20701 gctggtgaaa gaaaatcgcc gcgacacagg cgatgcctac agctttaact ggtcaatgcg
    20761 cattgcgcca gatttgcaaa tgccgtttga gccgtctcac gagaatatgg ctaatctgaa
    20821 gctttacccg gatcaacctg ttgaagtgct ggctgccgat ctgcgccgtg cgttctccgg
    20881 tattgtggcg ggtaacgtaa aagaagtcgg tattcgcgcc attgaagagt ttggtcctta
    20941 caaaatcaac ggcgataaag agattatgcg tcgtatggac gacctgctac agggttttgt
    21001 tgcccagcat cgtatgaagt tgccaggctc agcctacatc ccttgctacg aaatctgcac
    21061 gtaatctccc tcccccggtg acgtttgttg ccgggttttc tcgtttttgg tcacataccc
    21121 atcaatttat ttcatttgtt atatgaatgt ttcttaccac cctcacgcga taaatatcat
    21181 tgcagttaat atgtcataca atttatgttg taacgcaaac gtttccctat tttcataaac
    21241 cacattttat cgctatgaaa aagaaattat cgccatgatt aactaaaagt attgattttt
    21301 tcagttcaac ctacatatat tgcgcgcccc ggaagaagtc agatgtcgtt taatgggcaa
    21361 atattgccct taaattctct tttacttttg atttacagag taaagcgttg ggataatcta
    21421 tcttccaagt agattattgt atttgggatc aagatcactg atagatacat aacttgtgtg
    21481 tatctttccg ccctcaaatt attacggcgg taaatgatta agccatcgcc gatagacaga
    21541 tttcattttt acggtcaggc accttcccga gctgaactgg ctaaaagctg aattatttgc
    21601 attcctccag gagaaataga tggaaacgac tcaaaccagc acgattgcgt cgaaagactc
    21661 tcgtagtgcc tggcgcaaga cagacaccat gtggatgctg ggcctttacg gcacggcaat
    21721 cggcgcgggc gtgctgttcc tgccaatcaa cgccggtgtt ggcggtatga tcccgctgat
    21781 catcatggct atccttgcgt tcccgatgac attttttgct caccgcggcc tgactcgctt
    21841 cgtactgtct ggtaaaaacc caggcgaaga catcaccgag gttgtagaag aacactttgg
    21901 tattggcgca ggtaaactga ttaccctgct ctacttcttc gctatctacc cgatcctgct
    21961 ggtttatagc gtggcaatca ccaatacagt tgaaagcttc atgtctcacc agctgggtat
    22021 gacaccaccg ccgcgtgcga ttctgtcgct gatcctgatc gtgggtatga tgaccatcgt
    22081 tcgcttcggt gagcagatga tcgttaaagc aatgagtatt ctggtattcc cgtttgttgg
    22141 cgtactgatg ctgctggctc tgtacctgat cccgcagtgg aacggcgcag cactggaaac
    22201 gctgtctctg gacactgcat ctgcaaccgg aaacggtctg tggatgaccc tgtggctggc
    22261 aattccggtt atggtgttct ctttcaacca ctctccgatc atctcttctt tcgccgttgc
    22321 gaagcgtgaa gagtacggcg atatggcaga acagaaatgc tcgaagatcc tggcattcgc
    22381 acacatcatg atggtgctga ccgtaatgtt cttcgtcttc agctgtgtac tgagcctgac
    22441 tccggcagac ctggctgcgg ctaaagagca gaacatctcg attctgtctt acctggctaa
    22501 ccactttaac gcaccggtta tcgcgtggat ggctccgatt atcgcgatta tcgctatcac
    22561 caaatccttc ctcggccact acctgggcgc acgtgaaggc ttcaacggta tggtgattaa
    22621 atctctgcgt ggtaaaggta agtctatcga aatcaacaag ctgaaccgta tcactgcgct
    22681 gttcatgctg gtaacgacct ggattgttgc caccctgaac ccgagcatcc tgggtatgat
    22741 tgaaaccctg ggcggcccaa tcatcgcgat gatcctgttc ctgatgccga tgtacgcaat
    22801 tcagaaagta ccggcaatgc gtaagtacag cggtcacatc agcaacgtat tcgttgtcgt
    22861 gatgggtctg attgcaatct ccgcaatctt ctactctctg ttcagctaag tcctttcgcg
    22921 ccgctttcgg gcggcgcttc ctccgtttta acgcgatgta tttcctatga ttagcgtatt
    22981 cgatattttc aaaatcggca ttggcccttc cagttctcat accgttggac caatgaaagc
    23041 gggtaaacaa tttaccgacg atctgattgc ccgtaatctg cttaaagacg tgacccgcgt
    23101 ggtggttgac gtgtacggct cgctctctct gaccggtaaa ggccaccaca ctgatatcgc
    23161 cattattatg ggcctggcgg gtaacctgcc ggataccgtg gatatcgatt ccatccctgg
    23221 ttttattcag gatgtgaata ctcatggtcg cctgatgctg gcaaacggtc agcatgaagt
    23281 ggagttcccg gttgatcagt gcatgaactt ccatgcagac aacctttctc tgcatgaaaa
    23341 cggtatgcgc attaccgcgc tggcgggcga taaagtcgtt tacagccaga cttactactc
    23401 tattggcggt ggctttatcg ttgatgaaga gcattttggc cagcagaata gcgcaccggt
    23461 tgaagttcct tatccgtaca gttcagcagc cgatctgcaa aaacattgtc aggaaaccgg
    23521 gctgtcactc tctggcctga tgatgaaaaa tgagctggcg ctgcacagca aagaagagct
    23581 ggaacagcac ctggcgaacg tatgggaagt catgcgtggc ggtattgagc gcggtatttc
    23641 caccgaaggc gtgttgcctg gcaaactgcg cgttccacgc cgtgctgcgg cactacgccg
    23701 gatgctggtc agccaggata aaaccaccac tgacccgatg gcggttgttg actggatcaa
    23761 catgtttgca ctggcagtga acgaagagaa cgctgctggc ggacgcgtgg tgactgcgcc
    23821 gactaacggt gcgtgcggga ttatcccggc agtgctggcg tactacgaca agttcatccg
    23881 cgaagtgaac gctaactcac tggctcgtta cctgctggta gccagtgcca ttggttctct
    23941 ttataagatg aacgcgtcaa tttctggtgc tgaagtcggc tgccagggtg aagttggtgt
    24001 ggcgtgctca atggcggcgg ctggtctggc agaactgtta ggtgcaagcc cggcgcaggt
    24061 gtgcatcgcg gcggaaatcg ccatggagca caacctcggt ctgacgtgtg acccggtcgc
    24121 cggacaggtc caggtgccat gcatcgagcg taacgccatt gcggcagtaa aagcggtgaa
    24181 cgccgcacgt atggcgctac gccgtaccag cgagccgcgc gtctgcctcg ataaagttat
    24241 cgaaaccatg tacgaaacag gtaaagatat gaacgccaag taccgcgaaa cctctcgcgg
    24301 cggcctggca atgaagatcg ttgcctgcga ttaatcgctc tccagaggcc tcgttttgcg
    24361 aggcctcttc ccgatttctc atccagccgt agcctgttcc ggcatcgaat gttaccctta
    24421 tcgcctgatc tttaaggggg ttatcgtggc tgttcatttg cttattgtcg atgcactgaa
    24481 tcttattcgt cgcattcatg ccgttcaggg atcgccctgt gtcgaaacct gccagcatgc
    24541 gctcgatcag ctcattatgc acagccagcc aacccacgcg gtcgccgttt ttgatgatga
    24601 aaaccgcagt agcggctggc gtcatcagcg tttaccagat tacaaagcgg gtcgaccgcc
    24661 aatgccggaa gagttgcacg acgagatgcc tgcattacgc gccgcctttg agcaacgcgg
    24721 cgtcccgtgc tggtcaacca gcggcaacga agccgatgac ttagccgcca cgctggcggt
    24781 caaagtgaca caggccgggc atcaggcaac gattgtttcg acagataaag gctactgtca
    24841 gttactttca ccgacattac gtattcgtga ttacttccag aaacgttggc tggatgcgcc
    24901 atttatcgat aaagaatttg gcgttcaacc gcagcagttg cccgattact ggggacttgc
    24961 ggggatcagc agttcaaagg taccgggtgt tgcgggaatc ggaccaaaaa gcgccacgca
    25021 gctgctggtc gagtttcaga gtctggaagg gatatatgag aatctggatg cggttgccga
    25081 aaagtggcgc aaaaaattag aaacccataa agagatggcg tttctgtgcc gcgatattgc
    25141 ccgcttacaa accgatttgc atatcgacgg caatttacag caattgcggt tggtacggta
    25201 acggcgagcc ggatgcaaac gattatcaac acattgaatt taatgcactt atataccgga
    25261 taagacgcgg ctagcgtcgt atccggcatt atcacatcag cgcatttacc aggcggtatg
    25321 gtaaagctct acaatatcct caagcgttgc ttcacgcggg ttgccaccgg tacaaacatc
    25381 atccagtgcc gcctgcgcca gtgccggaat gtcttccttg cgtacaccaa catcacgcaa
    25441 atgtggcgga ataccgacat cacggttgag agcaaacacc gcttcaacag cggcattacg
    25501 cgcctcttcc aggctcatac cttccacttt cacgcccata acgcgcgcga tatcgcggta
    25561 cttctcaccg gtaaagtcag cgttatagcg catgacatgc ggtaacagga tggcgttcgc
    25621 aacaccgtgt ggagtgttgt aaaacgcgcc cagtggatgc gccataccat gcaccaaccc
    25681 taacccaaca ttcgagaagc ccatacccgc aacatactgc ccgagcgcca tttcttctcc
    25741 ggcatcctta tcaccagcaa ccgatcctcg cagcgcccca gcaatgattt caatcgcttt
    25801 aatgtgcagt gcatcggtta gcgcccacgc gccacgggta atatacccct caatagcatg
    25861 agtgagcgca tcgacacccg tcgcagcttt cagcgctgga ggcataccat ccatcatgtc
    25921 agcgtcaata aacgccacct gcgggatatc atgcggatca acgcaaacaa acttgcgccg
    25981 tttctcttcg tcagtgatca cgtagttaat ggtcacttct gccgcagtac ctgctgtggt
    26041 aggaattgcc agaatcggta cactgggttt attggtcggg gaaagccctt ccaggctacg
    26101 cacatcggca aactccgggt tgttgctgat aatgccaatc gctttacaag tatcctgtgg
    26161 agaaccacca ccaatagcga tcaggtaatc cgcgccgcta ttctggaata caccgagccc
    26221 ttctttgacg acagtaattg ttgggttggg cactacgccg tcgtaaatcg cccatgccag
    26281 ccctgcagca tccatcttat cggtcacttt cgccaccacg ccgcattgca ccagcgtttt
    26341 atcggtgacg atcagcgcct tctgataacc acggcgtttc acctcatcgg ttaaagcccc
    26401 aacagcaccc cgaccaaacc atgccgtttc gttcagaatc attctgttag ccatcatcct
    26461 tctccttgtt gctttacgaa attactcttc aatccgtaac ccataggttt tgaatttctc
    26521 cagcactacg gcaatctctt catcgctcag cactggcacc gggtccgtaa tcgccagggt
    26581 cgtcacgtaa agttgcgcca gcacttcaac ttcatgcgcc agccataacg ctttttccag
    26641 attcacctca caagcgataa gcccatgatg ttgtaacaaa gttgccttac gatttttgag
    26701 agccagcgca acatgttcag aaagttcgcg tgttccaaag gtcgcataag gcgcgcaagg
    26761 aatagaatta ccacctgccg ccgcaatcat gtagtgaata gcggggatcg gtcggttaag
    26821 aatggaaact gctgtgcaat gaacggcatg attgtgaaca accgcgttgg catccggtct
    26881 gctttgatag gccgccatat ggaaacgcca ttcgcttgag gggagctttc cttcctcatg
    26941 tttaccgttg ccatcaataa agacaatatg cgactccgtc agtttttcat atggaatgcc
    27001 tgtaggcgta atcagcatcc catcctgata acgtacactg acgttccccg ctgtcccctg
    27061 gttcagtccc aggcgggtca tttccaggca agtgtcaata atctgacgag caagtttatt
    27121 tcgttccatt agctacctct ctctgattca aaacagggca ataatgttgt tcctttcaca
    27181 ctattgaatt agccgtttaa ttacccacca tcttcttcct gattaacaag aaagaaattc
    27241 acaagcttat attttgtgac ctagctcaac taatcacagt aaataactgc aagttctctt
    27301 tttataaccc cattaaaaat gaccgctctt aaaaatattt atcaaaacgg tcatttttct
    27361 attcacccaa gccaggaatg cccgttttcg gcataaacaa ttaatacagt catctggttt
    27421 gtgtttttta tgatttattt tctgaaacgg gcatgaaatt tcgattatta aagtgatggt
    27481 agtcacataa agtcaccttc cagctaataa gtgtgaccgc cgtcatatta cagagcgttt
    27541 tttatttgaa aatgaatcca tgagttcatt tcagacaggc aaatattcac tgatatgaag
    27601 cccgaactcg ctggttttgc acttttgaaa acataaccga ttacgtgctt aagcttctga
    27661 acctaagagg atgctatggg aaacacatca atacaaacgc agagttaccg tgcggtagat
    27721 aaagatgcag ggcaaagcag aagttacatt attccattcg cgctgctgtg ctcactgttt
    27781 tttctttggg cggtagccaa taaccttaac gacattttat tacctcaatt ccagcaggct
    27841 tttacgctga caaatttcca ggctggcctg atccaatcgg ccttttactt tggttatttc
    27901 attatcccaa ttcctgctgg gatattgatg aaaaaactca gttataaagc aggaattatt
    27961 actgggttat ttttatatgc cctgggcgct gcattattct ggcccgctgc agaaataatg
    28021 aaccacacct tatttttagt ttgcctattt attattgcag ccggattagg ttgtctggaa
    28081 actgccgcaa acccttttgt tacggtatta gggccggaaa gcagtggtca cttccgctta
    28141 aatcttgcgc aaacatttaa ctcgtttggc gcaattatcg cggttgtctt tgggcaaagt
    28201 cttattttgt ctaacgtgcc acatcaatcg caagacgttc tcgataaaat gtctccagag
    28261 caattgagtg cgtataaaca cagcctggta ttatcggtac agacacctta tatgatcatc
    28321 gtggctatcg tgttactggt cgccctgctg atcatgctga cgaaattccc ggcattgcag
    28381 agtgataatc acagtgacgc caaacaagga tcgttctccg catcgctttc tcgcctagcg
    28441 cgtattcgcc actggcgctg ggcagtatta gcgcaattct gctacgttgg cgcacaaacg
    28501 gcctgctgga gctatttgat tcgctacgct gtagaagaaa ttccaggtat gactgcaggc
    28561 tttgccgcta actatttaac cggaaccatg gtgtgcttct ttattggtcg tttcaccggt
    28621 acctggctca tcagtcgctt cgcaccacac aaagtcctgg ccgcctacgc attaatcgct
    28681 atggcactgt gcctgatctc agccttcgct ggcggtcatg tgggcttaat agccctgact
    28741 ttatgcagcg cctttatgtc gattcagtac ccaacaatct tctcgctggg cattaagaat
    28801 ctcggccagg acaccaaata cggttcgtcc ttcatcgtta tgaccattat tggcggcggt
    28861 attgtcactc cggtcatggg ttttgtcagt gacgcggcgg gcaacatccc cactgctgaa
    28921 ctgatcccca cactctgctt cgcggtcatc tttatctttg cccgtttccg ctctcaaacg
    28981 gcaactaact gaacatattt tccgaataaa gtgaggaatc tgtaatgaaa aaaatcagct
    29041 taccgaaaat tggtatccgc ccggttattg acggtcgtcg catgggtgtt cgtgagtcgc
    29101 ttgaagaaca aacaatgaat atggcgaaag ctacggccgc actgctgacc gagaaactgc
    29161 gccatgcctg cggagctgcc gtcgagtgtg tcatttccga tacctgtatc gcgggtatgg
    29221 ctgaagccgc tgcttgcgaa gaaaaattca gcagccagaa tgtaggcctc accattacgg
    29281 taacgccttg ctggtgctat ggcagtgaaa ccatcgacat ggatccaacc cgcccgaaag
    29341 ccatttgggg ctttaacggc actgaacgtc ccggcgctgt ttacctggca gcggctctgg
    29401 cagctcacag ccagaaaggc atcccggcat tctccattta cggtcatgac gttcaggatg
    29461 ccgatgacac atcgattcct gccgatgttg aagaaaaact gctgcgcttt gcccgcgccg
    29521 gtttggccgt cgccagcatg aaaggtaaaa gctatctgtc gctgggcggc gtttcgatgg
    29581 gtatcgccgg ttctattgtt gatcacaact tctttgaatc ctggctggga atgaaagtcc
    29641 aggcggtgga tatgaccgaa ctgcgtcgcc gtatcgatca gaagatttac gacgaagccg
    29701 aactggaaat ggcactgacc tgggctgata aaaacttccg ctatggcgaa gatgaaaata
    29761 acaaacagta tcaacgcaat gccgagcaaa gccgcgcagt tctgcgcgaa agtttactga
    29821 tggcgatgtg tatccgcgac atgatgcaag gcaacagcaa actggccgat attggtcgcg
    29881 tggaagaatc acttggctac aacgccatcg ctgcgggctt ccaggggcaa cgtcactgga
    29941 ccgatcaata tcccaatggt gacaccgccg aagcgatcct caacagttca tttgactgga
    30001 atggcgtgcg cgaacccttt gtcgtggcga ccgaaaacga cagtcttaac ggcgtggcaa
    30061 tgctaatggg tcaccagctc accggcaccg ctcaggtatt tgccgatgtg cgtacctact
    30121 ggtcaccaga agcaattgag cgtgtaacgg ggcataaact cgatggactg gcagaacacg
    30181 gcatcatcca tttgatcaac tcaggttctg ctgcgctgga cggttcctgt aaacaacgcg
    30241 acagcgaagg taacccgacg atgaagccac actgggaaat ctctcagcaa gaggctgacg
    30301 cttgcctcgc cgctaccgaa tggtgcccgg cgatccacga atacttccgt ggcggcggtt
    30361 actcttcccg cttccttacc gaaggcggcg tcccgttcac catgactcgt gtcaacatca
    30421 tcaaaggcct gggatcggta ctgcaaatcg cggaaggctg gagcgtggaa ttgccgaagg
    30481 atgtgcatga catcctcaac aaacgcacca actcaacctg gccaaccacc tggtttgcac
    30541 cgcgcctcac cggtaaaggg ccgtttacgg atgtgtactc ggtaatggcg aactggggcg
    30601 ctaaccatgg ggttctgacc atcggccacg ttggcgcaga ctttatcact ctcgcctcca
    30661 tgctgcgtat cccggtatgt atgcacaacg ttgaagagac caaagtgtat cgtccttctg
    30721 cctgggctgc gcacggcatg gatattgaag gccaggatta ccgcgcttgc cagaactacg
    30781 gtccgttgta caagcgttaa tacattactc cctgatgtga tgcccggtcg ctccggctac
    30841 cgggcctgaa caagcaagag tggttagccg gataagcaat gttatccggc tatattgcag
    30901 gagcgattat gaaacaagaa gttatcctgg tactcgactg tggcgcgacc aatgtcaggg
    30961 ccatcgcggt taatcggcag ggaaaaattg ttgcccgcgc ctcaacgcct aatgccagcg
    31021 atatcgcgat ggaaaacaac acctggcacc agtggtcttt agacgccatt ttgcaacgct
    31081 ttgctgattg ctgtcggcaa atcaatagtg aactgactga atgccacatc cgcggtatcg
    31141 ccgtcaccac ctttggtgtg gatggcgctc tggtagataa gcaaggcaat ctgctctatc
    31201 cgatcattag ctggaaatgt ccgcgaacag cagcggttat ggacaatatt gaacagttga
    31261 tctccgcaca gcggttgcag gctatttctg gcgtcggagc ctttagtttc aatacgttat
    31321 ataagttggt gtggttgaaa gaaaatcatc cacaactgct ggaacgcgcg cacgcctggc
    31381 tctttatttc gtcgctgatt aaccaccgtt taaccggcga attcactact gatatcacga
    31441 tggccggaac cagccagatg ctggatatcc agcaacgcga tttcagtccg caaattttac
    31501 aagccaccgg tattccacgc cgactcttcc ctcgtctggt ggaagcgggt gaacagattg
    31561 gtacgctaca gaacagcgcc gcagcaatgc tcggcttacc cgttggcata ccggtgattt
    31621 ccgcaggtca cgatacccag ttcgcccttt ttggcgctgg tgccgaccaa aatgaacccg
    31681 tgctctcttc cggtacatgg gaaattttaa tggtccgtag cgcccaggtt gatacttcgc
    31741 tgttaagtca gtacgccgat tctacctgcg aactggatag ccaggcaggg ttgtataacc
    31801 caggtatgca atggctggca tccggcgtgc tggaatgggt gagaaaactg ttctggacgg
    31861 ctgaaacacc ctggcaaatg ttgattgaag aagctcgtct gatcgcacct ggcgcggatg
    31921 gtgtgaaaat gcagtgtgat ttattgtcgt gtcagaacgc tggctggcaa ggagtgacgc
    31981 ttaataccac gcgggggcat ttctatcgcg cggcgctgga agggttaact gcgcaattac
    32041 agcgcaatct acagatgctg gaaaaaatcg ggcactttaa agcctctgaa ttattgttag
    32101 tcggtggagg aagtcgcaac acattgtgga atcagattaa agccaatatg cttgatattc
    32161 cggtaaaagt tctcgacgat gccgaaacga ccgtcgcagg agctgcgctg ttcggttggt
    32221 atggcgtagg ggaatttaac agcccggaag aagcccgtgc acagattcat tatcagtacc
    32281 gttatttcta cccgcaaact gaacctgaat ttatagagga agtgtgaaat gctgaaaaca
    32341 atttcgccgt taatttctcc cgaactattg aaagtgctgg cagagatggg acatggagat
    32401 gaaattattt tttccgatgc tcactttccc gcccattcga tgggaccgca ggtgatccgc
    32461 gctgatggcc tgttggtgag cgacttgctc caagcgatta tcccattatt tgagctggac
    32521 agttatgccc cgccgctggt gatgatggca gcggtagaag gtgacactct cgatcctgaa
    32581 gtagaacgac gttaccgtaa tgcgctttca ctacaagccc cgtgtcctga catcatccgc
    32641 atcaatcgtt ttgcgtttta tgaacgggcg caaaaagcct ttgcgatcgt tatcacaggc
    32701 gaacgagcga agtacgggaa tattctttta aaaaaagggg taacaccgta atctcatatc
    32761 ggtacgcccg catgacgcgg gcggttatcg aatgatgggg tgaaaaatat gaaagcggca
    32821 cgccagcaag cgatagtcga cctgctgctg aaccatacca gcctgaccac ggaagctctc
    32881 tctgaacagc taaaggtcag taaagaaacc attcgtcgcg atctcaatga attacagacg
    32941 cagggtaaaa ttctgcgcaa tcatggacgc gctaaatata tccaccgtca aaatcaagac
    33001 agtggcgatc cctttcacat caggctgaaa agccattatg cgcataaagc agatatcgcg
    33061 cgcgaggcgc tcgcgtggat tgaagaaggg atggtgatag ccttagacgc cagttcaact
    33121 tgctggtatc tggcacgcca gttgcctgac atcaacattc aggtcttcac caatagccat
    33181 ccgatttgcc atgaactcgg taaacgcgaa cgcattcaac tgatcagttc cggcggcaca
    33241 cttgagcgca aatatggctg ttacgtcaat ccctcgctga tttcccaact taaatcgctg
    33301 gaaatcgatc tgtttatttt ttcttgtgaa gggatcgata gcagcggcgc actgtgggac
    33361 tccaatgcga tcaacgctga ttacaaatcg atgctattaa aacgtgccgc gcaatcgttg
    33421 ttattgattg ataaaagtaa atttaatcgt tcaggggaag cccgcatcgg gcatctggat
    33481 gaggtaacgc acattatttc tgatgagcgc caggttgcaa cttctttggt aacagcctga
    33541 cggaaagggt agcaggccga agacgacgcc ccggccttgc ctgttatcgc tcgtcgcgac
    33601 gaccacccac cgcagcccag atgcggcgga cgtgcaccgt cacttcttcg cgatcgtgat
    33661 acaactgccg cgcctgaatc tgagcattta tgccatgttc atcaagctgt gcctgaatat
    33721 acgccagatt gtgtgatact tcttcgtagc gttttttcat cggcagtttg agattgaaaa
    33781 tggtttcacg gcaccagcca ttaaccagcc actgcgccat caatgccgca actttagccg
    33841 gtttttcaac catatcgcat accatccagg agatattgct gcgtgtcgga cggaatttga
    33901 aaccgtcttc ccgcagccac gtcacctgcc cggtatccat cagactttgc gccatcgggc
    33961 cgttgtcgac ggaataaacc cacatgttgc gcttcaccag ttggtaggtc cagccgccag
    34021 ggcaagcgcc taaatccacc gcccacatcc cgttcgccag gcgttcatcc cactcatccg
    34081 caggaataaa cacatgaaat gcctcttcca gtttgagcgt ggaacgactc ggcgcctctg
    34141 ccggaaattt caggcgcgga atgcccatat agaacggcga attattgttg ctgtatgagt
    34201 aaccggtata gcagcagcct ggtgcaataa agaatacatg cacaaccgga cgcttcggcg
    34261 tttcatagtt cgccagcacc ccggcatcgc gcaaggcagc gcgtagcggc acggtgaatt
    34321 tacggcagaa tttcagcaac tctttgcttt cgttggtatc ggcaacttca acacgcagtt
    34381 caccgccctt ctctactacg ccctgcaaca tgtcgacaat gggggtaata cgatcttctg
    34441 gcggcaaatg ctgcaagagt tcccccacca caaaccactg gcgggcaaaa attaatgaac
    34501 tgaacggcag ctcacggatt aacttatcgc catcatcagg ttgataacat tcataaatga
    34561 cataacccgc attctctttc acgcgggcaa aaccgaaaat ttcccgctgg ccggctttat
    34621 cggtaatttc tgcggcgcac tctttttcaa agcccggacg gcacagcaat acaaccttat
    34681 tcatgactta cgcccttgcg ctttaaacgg atagcaccaa ctaacattaa cgcccagccc
    34741 gcgaggaagc tcacgccgcc aaccggtgtg acaaatgccc acaaacgcag atgggacagc
    34801 gccaggcaat aaaggctgcc gctgaacaac accgtgccta acgcgaggaa aacgctactc
    34861 cagtaaaacc agatactgat gcgacgctgc attgccaccg ccagacctaa gatcgccagc
    34921 gtatgaaacg cctggtattc gaggccggtc tggatccagc ccatctcaac ggcccccatg
    34981 gttttactta acacatgcgc gccaaaagcg cccagagcca caaaaatgaa gccgctaatg
    35041 gcggcgaaaa tcagcataaa acggctggtc atggtcgtac cctacgtaaa ttattgttca
    35101 taacgaaagc ggaatttttc ttgttcagca gcggctttcg ccaggatcca ttggcgaaag
    35161 gcggctattt tacccagttc tgcctggctg tcatgacaaa ccagataaaa agcgttttta
    35221 ctgaccagaa catcattaaa cgggcaaaca agacgtccgg cctcgatttc agattgcgcc
    35281 atcacgttat ttgccagcgc cactccctgc ccgtgaatag ccgcttgcag caccatggcg
    35341 ctatgactaa aaattggccc ttgctgaacg ttgatatgat ttaaccccaa ctgtcgggta
    35401 tatgtctgcc agtcacggcg tgacgcatca tgtaataacg tatgtttagc cagatcttcc
    35461 ggggtcttca agggtttttc gcctgtcagc agtagcggcg aacacaccgg caataaatat
    35521 tcggcgtaca gtttttccac ccgtagcccc ggccagttgc cccgaccata aaatatcgcc
    35581 acatcaacat catccgccag cttatcttcc tgacgatcaa ccgcctggat tcgaacgtca
    35641 attcccggat aagctgaatt aaagctggaa agtcgcggaa ccaaccaatg aatggcgaaa
    35701 ctggggagta aactgaccgt caacgccccc ttggcgctac gggcctggag tttacgcgtc
    35761 gcttcggtta attgcgaaaa tatctctttg atatcgagga aatagctttg cccttcctcg
    35821 gtcagcagga gtgaacgatt acggcggcgg aacagtttta gccccaaaaa atcctcaaga
    35881 gacttgattt gatgacttac tgcggcctgg gtcacaaaaa gctcttctgc tgcgcgagtg
    35941 aaacttaaat ggcgtgctgc ggcatcaaaa actcgtaagg catttagcgg tggtaatcgt
    36001 ttagacatgg cttttaaact ttgatgttaa attaatttaa caattagatc acactgtgta
    36061 acctattagt ttttttaatc tgagccatta taaattgtcc gttgagcttc taccagcaaa
    36121 tacctatagt ggcggcactt cctgagccgg aacgaaaagt tttatcggaa tgcgtgttct
    36181 ggtgaacttt tggcttacgg ttgtgatgtt gtgttgttgt gtttgcaatt ggtctgcgat
    36241 tcagaccatg gtagcaaagc tacctttttt cacttcctgt acatttaccc tgtctgtcca
    36301 tagtgattaa tgtagcaccg cctaattgcg gtgctttttt ttaccttgcg atcgcgaatt
    36361 actgatccag ttcgaccatc tctttcacgt cagtacggtt gatctgctgt ttgttgccat
    36421 tagcgtcttt atacgaaatc ataccggtat cgttatcagt ctgtggtttg ccgtcagaga
    36481 cgatggtacg tccgtcattg gtgtgcatca catagttcga accggaacag gcgctcaggg
    36541 caaaagtcag catacaggca gaaataattg cggcagtctt tttcatcatc ttctccttaa
    36601 agcgattaat aaatcttaat agtattgatt aacaggctaa aattaacgcc taacactatt
    36661 cagcatatgt tacttacgcg gcttcgccag gatatccaga taattctgat ggttagcact
    36721 ctccttgtat caaagtgaat tttgcgtcac gatcggtgca tcaagccgag gagtaccatg
    36781 aacgttttta atcccgcgca gtttcgcgcc cagtttcccg cactacagga tgcgggcgtc
    36841 tatctcgata gcgccgcgac cgcgcttaaa cctgaagccg tggttgaagc cactcgacag
    36901 ttttacagcc tgagcgccgg aaacgtccat cgcagccagt ttgccgaagc ccaacgcctg
    36961 accgcgcgtt atgaagctgc acgagagaaa gtggcgcaat tactgaatgc accggatgat
    37021 aaaactatcg tctggacgcg cggcaccact gaatccatca acatggtggc acaatgctat
    37081 gcgcgtccgc gtctgcaacc gggcgatgag attattgtca gcgtggcaga acaccacgct
    37141 aacctcgtcc cctggctgat ggtcgcccaa caaactggag ccaaagtggt gaaattgccg
    37201 cttaatgcgc agcgactgcc ggatgtcgat ttgttgccag aactgattac tccccgtagt
    37261 cggattctgg cgttgggtca gatgtcgaac gttactggcg gttgcccgga tctggcgcga
    37321 gcgattacct ttgctcattc agccgggatg gtggtgatgg ttgatggtgc tcagggggcg
    37381 gtgcatttcc ccgcagatgt tcagcaacta gatatcgatt tctatgcttt ctctggtcac
    37441 aaactgtatg gcccgacggg tatcggcgtg ctgtatggca aatcagaact gctggaagcg
    37501 atgtcgccct ggctgggtgg cggcaaaatg attcacgaag tgagttttga cggcttcacg
    37561 actcaatctg cgccgtggaa actggaagca ggaacgccaa atgtggctgg tgtcatagga
    37621 ttaagcgcgg cgctggaatg gctggcagat tacgatatca accaggcaga aagttggagt
    37681 cgcagcctgg cgacgctggc ggaagatgcg ctggcaaaac gtcctggctt ccgctcattc
    37741 cgctgccagg attccagcct gctggccttt gattttgctg gcgttcatca cagcgatatg
    37801 gtgacgctgc tggcggagta cggtattgcc ctgcgggccg ggcaacattg cgctcagccg
    37861 ctactggcag aattaggcgt aaccggcaca ctgcgcgcct cttttgcgcc atataataca
    37921 aagagtgatg tggatgcgct ggtgaatgcc gttgaccgcg cgctggaatt attggtggat
    37981 taatgacgaa cccgcaattc gccggacatc cgttcggcac aaccgtaacc gcagaaacgt
    38041 tacgcaatac cttcgcaccg ttgtcgcaat gggaagataa atatcgccag ttgatcatgc
    38101 tggggaaaca gcttccggct ttgccagacg agttaaaagc gcaggctaaa gagattgccg
    38161 gatgcgaaaa ccgcgtctgg ctgggatata ccgtgactga aaacggtaaa atgcacttct
    38221 ttggcgatag cgaagggcgc attgtgcgcg gcctgctggc ggtgctgttg actgccgttg
    38281 aggggaaaac cgccgccgaa ttgcaggcac agtcgccgct ggcattgttt gatgagctgg
    38341 gattgcgtgc gcaacttagc gcctcacgca gccaggggtt aaatgcgtta agcgaggcga
    38401 ttatcgctgc gacgaagcag gtttaagcat agaaccgggt cggataagac gttcgcgtct
    38461 catccgacct gattgtttaa ccctgacgcg ccgctttcgc catcatcttc ttcagcgcat
    38521 gagaaaccgc aacaaaccca aaggtggcgg tcaccatcgt tgccgcgcca aatccagatg
    38581 cacaatccat ccgcttcggc ccttctgccg tggctttcat cgcacacaca gtaccgtctg
    38641 actgcgggta caccagcgct tcagtagaaa acacgcaatc gacgccgagc ttacctttac
    38701 tgtttttcac tacgccaaaa tcgcttttca ggcgctcgcg caacttcgcc gccagcggat
    38761 cctgaatcgt tttcgccaga tcggtaacct gaatctgcgt cgggtcaatc tgcccacccg
    38821 cgccaccggt cgtgaccagc gggattttat tgcgccgaca ataagcaatc agcgccgctt
    38881 tgggccgtac actatcaatg gcatcaatca cgtacgaata acccacgctc atatactgcg
    38941 ctacgttatc cggcgtcacg aaatcatcca ccaccgttac acggcactcc gggttaatct
    39001 ggcgaatacg ctccgccatg acttccgctt ttgccagccc gacgttatcg cgcagagcgt
    39061 gaatttgccg attggtattg gtgacgcaca catcatccat atcgataagc gtgattgcgc
    39121 caatccccgt gcgcgccagc gcttccgccg cccaggaacc gacaccaccg ataccaacca
    39181 cacaaatatg cgcgtcagca aacagttgca acgctttttc accatacaga cgcgctgtgc
    39241 caccaaaacg ctgacgccat gcatcactaa ttaccacaga catagcacct cagaattaaa
    39301 aagggtgagg ttatccctca ccctgtcgta tccgtaaaaa cggtatacag aataacacat
    39361 cagccgctaa agacgttacc tgcgcccggg gcggttttca gcacccagac acgtccatag
    39421 tggttgtacc aacctgcgcg gtgtccggct tccggcccga tcccttgata gatatcgaag
    39481 tgttggcctt tgattgcacc accgacatcc agcgccacca tcagacgcag ttcgtactga
    39541 ccattaaatt tgccgttatt atccagcaac ggcacttctg ccagcaaggt agtacctggc
    39601 ggaataatgg aacgatcaga ggcaactgac gcgcgaccaa ccagcggcac cgcacttgcc
    39661 cctttcaccg gtgcaaaaga ttgcggttta aagaagacga aagacgggtt ctgttccagc
    39721 agctcgcgaa cctcggcttc actgtgtgtt tcgccccagt gacgaatcgc ctgcatcgac
    39781 atatcttctt ttttcacttc gccacggtcg atcagcacct taccaatgct gcgataggca
    39841 tgaccgtttt tccctgcata gctgaaaaag ttaagcggac tgccatcacc aaagtcgata
    39901 tacccactac cctgcacatc cataatgaag ttatccatca gggagttact gtaagcgaga
    39961 atatatttat cactcaatgc ccccgcgtag atctccgcac gagacggcag acgaccacgt
    40021 tttggcggca tacggtaaat aggatactgg aactcgccct ggcgggtatg gcgcgcctga
    40081 attaccggcg tgtaatagcc tgtaaactgc acgttaccat agttgtcggc accttccatc
    40141 tgccaggcat caatgccgaa ctggcgcata ttgcgggtat caccgcctgc gcgcagccac
    40201 tcttgcaccg cgttataaac attactctgg ttgccataca gacgcggtga cgaattacgg
    40261 atatggttaa tttgctcggc aaaatcaccg gcgttaatcg gcgcgccaac ggcatctggc
    40321 tggttcacca gagagaaagg ctgggtaaat ttcccgtctt tatattgctg tccgcgatcg
    40381 gttggtttgg aagagcaggc ggcaagcatt gccacaaccg tgcccataag aaggtacttt
    40441 acccaacgtc ctttcattgt tctcttcttc aggttaaaaa ataaggcgca acgaagataa
    40501 caaaccgccg cgtttaatga aatgagcagc ctctccctga cgcaaatttt gcacaaaaaa
    40561 taggctttag tgatttgttt ttgttcaaaa tcatgccaga tccgtgatcg gggtaaaaaa
    40621 aaggttgcat gaaaacgcga gcggagtata gtgcgcatcc acggaggcgg ggtggagcag
    40681 cctggtagct cgtcgggctc ataacccgaa ggtcgtcggt tcaaatccgg cccccgcaac
    40741 caattaaaat ttgatgaagt aaagcagtac ggtgacgcgg ggtggagcag cctggtagct
    40801 cgtcgggctc ataacccgaa ggtcgtcggt tcaaatccgg cccccgcaac caattattga
    40861 acaccctaac gggtgttttt ttgtttctac tctcccataa aaaaagcgcc attcagcgcc
    40921 tttttatcat ccccttctcg ccagcgttgc cccatcggca aaatacgctt taatccccgc
    40981 aagaatagac tccgcaactt cctgctggaa agtcgccgtt ttcagtttac gctcttcctc
    41041 aacgttactg ataaacgccg tttcgaccag aatggaggga atatctggtg cctttagtac
    41101 ggcaaacccg gcctgttcaa cttgattttt atgcagcttg ttgattttac cgagcttatt
    41161 cagcactgct ttaccaaact taaggctgtc agcaatggtc agcgactgca ccatatcgaa
    41221 catggtgtgg tcgacatagc ggtcaccgct tttgctcacg ccaccaatca agtccgaggc
    41281 gttctgggtt tgtgccagat attttgccgc agtactggtt gcacctttgg ttgagagcgc
    41341 aaacaccgag gaaccgctcg gctggcgact ggtaaaggcg tcggcatgga tagagacaaa
    41401 caggtcagca cgctgtttct gggcttttgc tacgcgcact tgcaacggaa tgaagatgtc
    41461 ttcattgcgc gtcatgtaca ccttcatatt gccctctttc tcgatcagag agcgcaaacg
    41521 gcgagctatt tgcaatacca catctttttc gcgcgttttg tatttcccca ccgcaccgga
    41581 gtcttcgcca ccgtggccag ggtcaagcat aatgacaatc ggacgatcgc gccctgcttt
    41641 acctggttgt ggaccacttt gcgctggcgg cacctgcttt tcgaggtcgc ctttgttgta
    41701 atcctccagc agcgccagca gcgggtcctg catatcctgt gcattggccg gatagaggtc
    41761 catcaccaga cgctctttaa acccggcgac cggcgcaagg gcaaacagct gcggttttac
    41821 gttttgcttt aattcaaaaa ccatccgtac ggtttgcggg tcaaattgcc cgacgcgcgc
    41881 cgacttgatg aacgggtcgt ctgcacgaat ttgtgccgcc atccccttga gtaccgagtt
    41941 caggtttaca tcttcgatat ccaccaccac gcgttcaggg ttactcaacg cgaactgctt
    42001 atatttcagc tgacgatttg attctaccgt cacgcgggtg tagctggacg caggccagac
    42061 gcgcaccgcc acgacctggc taaccgcagc caggctgacc tgacttacgc tcaatagcca
    42121 catagcgccc gcgccttgca gtaaacgacg acggctgatt gcagtgttgg atcctgacat
    42181 gcctctcccg agcaaaagaa atctaagctg tgtaacaagt aaacgactaa tttgaccgga
    42241 aactttagcg aaagacgcat aatctgtcat ctaataaacg gtaaacattc tttttatatt
    42301 cacggcatta ctgataaaaa agtcgctctc gcataaaatt tacacttgca ccctgcgaaa
    42361 aaacagaata aaaatacaat aatttcgaat aatcatgcaa aggggtgtgc cgtggtaaag
    42421 gaacgtaaaa ccgagctggt cgagggattc cgccattcgg ttccctatat caatacccac
    42481 cggggaaaaa cgtttgtcat catgctcggc ggtgaagcca ttgagcatga gaatttctcc
    42541 agtatcgtta atgatatcgg gttgttgcac agcctcggca tccgtctggt ggtggtctat
    42601 ggcgcacgtc cgcagatcga cgcaaatctg gctgcgcatc accacgaacc gctgtatcac
    42661 aagaatatac gtgtgaccga cgccaaaaca ctggaactgg tgaagcaggc tgcgggaaca
    42721 ttgcaactgg atattactgc tcgcctgtcg atgagtctca ataacacgcc gctgcagggc
    42781 gcgcatatca acgtcgtcag tggcaatttt attattgccc agccgctggg cgtcgatgac
    42841 ggcgtggatt actgccatag cgggcgtatc cggcggattg atgaagacgc gatccgtcgt
    42901 caactggaca gcggtgcaat agtgctaatg gggccggtcg ctgtttcagt cactggcgag
    42961 agctttaatc tgacctcgga agagattgcc actcaactgg ccatcaaact gaaagctgag
    43021 aagatgattg gtttttgctc ttcacagggc gtcactaatg acgacggtga tattgtctca
    43081 gaacttttcc ctaacgaagc gcaagcgcgg gtagaagccc aggaagagaa aggcgattac
    43141 aactccggta cggtgcgctt tttgcgtggc gcagtgaaag cctgccgcag cggcgtgcgt
    43201 cgctgtcatt taatcagtta tcaggaagat ggcgcgctgt tgcaagagtt gttctcacgc
    43261 gacggtatcg gtacgcagat tgtgatggaa agcgccgagc agattcgtcg cgcaacaatc
    43321 aacgatattg gcggtattct ggagttgatt cgcccgctgg agcagcaagg tattctggtg
    43381 cgccgttctc gcgagcagct ggagatggaa atcgacaaat tcaccattat tcagctcgat
    43441 aacacgacta ttgcctgcgc cgcgctctat ccgttcccgg aagagaagat tggggaaatg
    43501 gcctgtgtgg cagttcaccc ggattaccgc agttcatcac ggggcgaggt tctgctggaa
    43561 cgcattgccg ctcaggcgaa gcagagcggc ttaagcaaat tgtttgtgct gaccacgcgc
    43621 agtattcact ggttccagga acgtggattt accccggtgg atattgattt actgcccgag
    43681 agcaaaaagc agttgtacaa ctaccagcgt aaatccaaag tgttgatggc ggatttaggg
    43741 taagcgatga aaatcgccgg atgcggcagg cgtaacactc gtgcgtcgca tccggcaatt
    43801 acgtttatcc ccgcgaacta aacaacgccg ccagaccact gcgccgctca gtacgagtgg
    43861 cgattgccgc acttaatata cgctcatcgg catacagcga cagacggcga cgcgcgcggg
    43921 tcaccgcggt gtataccagc tctcgcgtta ctatcggcgt gcgttggctc ggtaaaatca
    43981 acgccgcatg gtcgaactcc gatccctgcg atttatgtac cgtcatcgcc cacgtagttt
    44041 cgtgctctgg caagcgactc ggttgcacag acttaatatt gccgtccggc atcgcaaacc
    44101 agacgcgcgt cccctgcccg cgatccagcg caataccgat atcgccatta aacaacccaa
    44161 gcgcgctgtc attacgggca atcatcaccg gtcgaccttc gtaccaacga gagtgcgaat
    44221 gacgatgaat tttgcgcttc tgttgcataa actgctcaat tcgctcattc agtccagcca
    44281 cgccaaatgg cccttcccgc agggcgcaca aaagttggta ctcattgaac gcctgaatga
    44341 ttaaatccgg ctcggcacgc gcttgcagca gatccagata acgtccataa cccgcaagag
    44401 cttcctcaag catcgcaata taatcttcgc cgctctgtaa aagccgtttt tcaatatcag
    44461 taaaatcctg ctgaaaaacg gttttcactg ccgttttatc accacggttg atcgccgcag
    44521 ctaactgacc aatgccagaa tcgctgccga aacgatagct tttttgcagc aggcagagac
    44581 tgtcgcgcaa agatgccgct tctgtgccgg ttcctgccgg aacgtgagtt cccgtcaggc
    44641 ggcttagctg cccggcacgc tcggcggtaa agccggcatt ggcataagcg cagatatcgc
    44701 ccagcacagc cccagcctca accgaggcca gttgatcacg atcgccgaga aagatcactc
    44761 gcgcatgatc gggcaaggcg tcgatcagtc tcgacatcat aggcagatcg atcattgacg
    44821 cttcatctac caccagcaca tcaagatgca gcgggttacc ggcatgatga cgtaaacgct
    44881 ggctacccgg ctgcgcgccc agcaatcggt gcaaagtgct ggcatcttcc ggaatgcgtt
    44941 tcttttgttc atcggtcagc ggtaactgtc gcaaagcctt gccgagagat tcggttaagc
    45001 gcgcggcagc tttacccgtt ggtgcagcca gacggatacg gcagcgttcg ccgtcggcca
    45061 tttgaattaa cgctgccagc aacttcgcca cggtggtcgt tttaccggtg cctgggccgc
    45121 cggaaatcac cgagatccgc cgcgtcagcg ccactgctgc cgccactttt tgccagttaa
    45181 tttcatcgct tactggaaaa agtttgtcca gggtttgcgc cagtagagct tcatcaacct
    45241 caatggcatg attcacctca ttgaagaagc gcgctaccgt ccgctcgtta caccacatgc
    45301 gattcaaata aagacgatcg ccacagagga tcatcggcgt gggttcatct cccctgctga
    45361 ccgcttgaga agccagcaag cattcttccc aattttgtag ctcaccgatt tcactgacac
    45421 aggtcgccaa cagcggatgc gacgcctcgt tattttccag tcgtgaaagc ggcaaacaga
    45481 cgtgtccctc tccggcatca tgacttaaca gtgccgccgc gagggtgacg gcaggatgtt
    45541 catctcccgc cacggtcagg gcaaattgca catccagcgg gcgtagctgt ttgtgctcca
    45601 cagcttccag taattgcttt tgcaatttca ttacgcctcc tccagggtca taccggcaaa
    45661 catctcatcc atcagggcaa tcaacccggc gttgggtcgg gttgtgtaaa tcccttgttg
    45721 cggatgttct ttatcaacgc cacgcaggaa cagatagata acgccgccaa agtgacgatc
    45781 atagtcgtaa tcagcaatgc gatggcgcag ataacgatgc agcgccaggg tataaagctg
    45841 atattgcaga tcatagcggt gtgcctgcat cgctgctgcc atagcctgtt gggtgtaagc
    45901 cgaactgtct tcacccagcc agttggattt atagtcgagc aggtaataac gcccttcgtg
    45961 gcggaatacc aggtcgataa agccttttaa catgccgcgt acctgcatga actccagcgg
    46021 cgggcagcca gcggatagcg ggtcaaactg gcgaattagt gcatcaagct ggctggcgat
    46081 gagcggttcg ctaatcggca ggtaaaactc catctccacc tgtttatcgc ggtcggaaag
    46141 ctgactcagg ctaacacccg tttcattgag aggtgcctgg aggacagccg tgatccactc
    46201 ggtcaacacc ggttcccact gcgattcaaa gccgccgagt tccagttttt cctgtaccca
    46261 gttcgggtca accggctggg taaaatcgag gtcttcaaac aaactgtgca agaatgtccc
    46321 cggtgacgca ccgcgcggga actgatgcgg tgttaacgtc ggttcttcaa cgacgctgac
    46381 cacgcctgcg gcatcaacat ccagccgtgg catcagatcc tgagcgatac cgtgaccacg
    46441 ctgctgcaag ccggagtagc tggtgacacg ccagttatcg ccgggcaatc gttgtagtgt
    46501 cctcgcattc agttctgcag ttaaagcatc attaacctgc cagggctggt tatcaccagt
    46561 ttgtgccgtt tgccaggcaa tatcatcatc gcataacgct tcaatacagg tgcgaagccc
    46621 tgccgcatct tgcggttccc ctttttgcag caaacgcccg agcgcacttt ggtggacgtc
    46681 ggtgtcacct tttttatcgc cacgacggcg caccagcggt gcaacgccga gactgcaatg
    46741 ccaaaccgaa cgcgtcagcg ccacgtaaag caaacgcaga tcttccgcca gacgttcgac
    46801 ctccgcgagg tcgacgcttt ctggcgcagc attaagatcc aaaactgcct caaacgagtg
    46861 gcgatcgtga taaaacgcct gatcctggac gcggaaattg gtaataaatg gcaaccagac
    46921 cagcggatat tccagccctt tcgatttatg gatcgtgaca atctgcacca gatgtttatc
    46981 actttcgagg cgcatttgtt ggctggaggc attactgtct ggctcgagga tatgttgcga
    47041 taaccagcgt accagcgcat gttcactttc cagctgcgtt ccggcttctt gaagcagttc
    47101 gctgatatgc aagatatcgg taagacgccg ctcaccgcct gccgttgcca gcaagttttc
    47161 tgcaatgtta cgcgccgaca tcagcgcccg cagcatcggc ataacgccac gtttgcgcca
    47221 gatttgccga taaccatcga actcttcgac taccgcatcc cacgcatgtt cgtcattatt
    47281 cagcgtttca atatccagcg cattcagccc catcattgac gttgccagcg cactgcgcag
    47341 ggtgttctca cgttcgggcg tcatcaccgc ctgcaacagc caaagcattt cctgcgcttc
    47401 cagagtttca aaaacactat cgcggttcga aaggtaaacg gaagggattt ccagcaacgt
    47461 taaggcatcg cgcacctggg cggcctcctg gcggctgcgc accagcacgc tgatgtccga
    47521 agcacgcacc ggacgcgcgt cgtcgccgtt catcagcaac gcttcgcccc gctgtccggc
    47581 ttgtagccag tcgcggattt gcgcagcaca tacctgcgcc atggtacttt gatagtcgcc
    47641 aacgccgcag ctttcgcctt ccatcagcca cattttcatc gcaggctgcg tttcaccttt
    47701 aaagacaaaa cgtaacgcct gatttttccc ggctgatttc actggaataa acggtatttc
    47761 gcgaaacatg aacgcgtcat cagtctggct gaaaagcttg ttcacactgt tcaccattcc
    47821 aggtgcggag cgccagttgg tgtctaaagt gtagtgggcg tgaacttcac tacgcgcctt
    47881 catataagtg aagatatccg caccccggaa tgcatatatg gcctgcttcg ggtcgccaat
    47941 cagcaacaat gcggtttctg gctgatggtg ccagatacgg cgaaaaattc ggtactgctg
    48001 ggggtcggta tcctgaaatt catcgatcat tgccaccggg aatcgcgtac ggatcgccgc
    48061 tgccaacacc tcgccgcttt cgctacgcag cgcggaatcg agccgactta acatgtcatc
    48121 aaaacccaat tcgccacggc ggcgtttttc acgcgctact gtttcgcgga tctcagccaa
    48181 tgcgcgggtg atcaccagat cacggatcga caatggttct gcaagcagtt gatcgatcgc
    48241 ctcaaacagt ggatgtcgcg gggtttcccc cccggctttc gtgcgatctt ctaagaaacg
    48301 ctgggagaat ttttccagcg actccggcaa ctgataactg tttgtctctt cttctgccca
    48361 ggcgctgatc ttgtcgatcc atttagcctg attgctacgg ttaaacttgc gtcgatcaat
    48421 accagaagat tcgatcagcg catccagttc acccactgcg tcgcgccact gctgttttac
    48481 cgtatcaata cgcgccacaa tttgcgcgtg acgggaagct agcgtttcat catcgggcgg
    48541 cggtgctttg ataaccggcg cttcgccttg cagataacga ttaatatcgc gcagcaacgc
    48601 ctgcggccct ttccaggttt caaagacgac ctgggctatt tcacgcggca gcgggtagca
    48661 gtggcgacgc cagaaatcgg cgcaggcctg gtagcgtagc agagactcat cttcaatcag
    48721 ctgctgctca aacagcatgc cggattcaaa ggcattcagg ttgagcatgc gctggcaaaa
    48781 gccgtgaata gtaaagactg ccgcttcatc catctgccgt tcggctaaca acaaccactg
    48841 cgcggcttgc gctttatcgt cgatctcttc cagcaggtgt tcgtacaatg gattgtcggt
    48901 ggtttcacgc agacaggcga tgcgcaactc gtggatattg ctacggatac gaccgcgcaa
    48961 ttctgccgtg gcagcctcgg taaaggtgac caccagcagt tcttcaacgg tcagcgggcg
    49021 gggaaaggcg gcggaaccgc ctagtccaag taacaggcgc aaataaagcg ccgcaatcgt
    49081 aaaggttttg cctgtgccgg cagaggcttc aatcaggcgc tcgccctgta agggcaagcg
    49141 caaaggatct agtgtctcgg cgacatcact cattcttttc actcatcagg ggcattgttt
    49201 gctgcaacgc gctgacgttc tcccacactt tccagccttc aggatgcaca tattcggctt
    49261 tcccgttttg gctgccggaa atctgcgaca ggatagccat gccttgcggc tcgactaccg
    49321 cctgatggaa gaaatcggcc agtttttgcg gcgtcagcag tttaatctgg gccacgattt
    49381 tatcacgcga atcgaagcgc atattgccgc gatcgaaatc tttacttaac ttcgatgctt
    49441 cttcgccgag cgtttgcggt gcctgcagca tctgggtaat taccgcctgc tggatttgcg
    49501 caaactcatc tggcttcatc gctcgcaatt ttgcctctgc ggttgggaaa aacgccttgt
    49561 aacgctccca caagaatgaa ggctgtttat cattgctttg caaaaggaag cccatgcccc
    49621 actgacgccc cacgctcatt ggaaacgcaa acacggcata gcccaattgt tcttcggtac
    49681 gcaactgatt gtagaaccac ggctgtacga tctgccccaa cagagagcta taggctgagc
    49741 tggtgtattc atcgtagcca gtcggtacaa ataccgctgc cagtgcggag tcggtgctgt
    49801 taccggcttt ttcaaagatg acggattgtt ttttatcgac cactacatct ttgtttcgac
    49861 accactctga accatcagcg cccaactgtt tttgcacatc gcgtgccagc gttgttgcct
    49921 gggcctcggt catgttgccg ataaccataa actctggtcg agcccctgat tttaaggcgt
    49981 cgcgataagc cagcacctct ttcaacgtaa tggagggcaa aattttacgc cgttcatctc
    50041 gcgagaagta cggcacttgc gagagcatct gcgcgggcat aatcgcctgc tcaaacgctt
    50101 tacccttttc tgcggaatcc atcatctggt tataccagga cttcgcctgc tcaagctgat
    50161 cttccgtagc ggtatagcta aagtacccct cgagcaatgc ctggaacagc tgcggcagac
    50221 gctgggtgta accattagca ttaaccataa ggccgttgtt agcgttggtg gaaaaactta
    50281 tgccaccaac cgacgcctgg ttgcttaact gatcaagcgc cagccctgcg agataatcat
    50341 tgagcgcaaa catcacctga ttgcgggcgc tgtccatggc tttcggatta cgcaaaatca
    50401 ggctgacatc agctttgggt tcgctggaaa aataacggct tggcgcatac accacgcgca
    50461 gattcgactc atcaacaatc agctctggat ggtcatattt cttctctgac ttaatcagcg
    50521 agaaatcgtc aggaatatag gggttaagct ccggtaagga gagcgcaata ttggcagctt
    50581 tttgctgcca gtcagcgaaa gtttgttcgc tgattttatc gacctgatac ggcgcatcga
    50641 caaaataagc cgttttattg tgcggctctt tcgggctgat ataccagata cgcgcattct
    50701 gcggcgtcat catcgccaga cgttctttta ctgctttagc atcgtaccga tcggcaatat
    50761 tgactgcatc cagcgtatgc tcaaccggaa cgcgaatcat ggtatctgcc agccattcga
    50821 cgtaatccat atcacgggtg atcgacggat aacggaagtc gatatccagc acattcgcca
    50881 gttcatcgaa gtattgttta tcaatgcctt tttcacgtaa cagattgaga tagctaaaaa
    50941 ttgccgccac aacctgatcg cgattagcca ggcctttatc ggttaaagac gcagagatcg
    51001 ctaatacgcc gctgttgccg ttgacgatag gatcggagtt ggcgctaatg ccctcaacta
    51061 atccctgctt ttgcagccag tcggaaagtg tacctgggct gcgattacca atcagatagg
    51121 taatcaattc atccgtttta ctacggaact tcgctgagtt gttatcgatg cgaaactcaa
    51181 cgcgcagaac tttacgcggc agtgccggga cgtaatgaat gataatgccc ttttgcgcgt
    51241 cggtgactac cggcacggtg atttccggtt ttttgctctc tttgttcggc acgcgaccaa
    51301 aggtgtccgc cgccattttt gccaactccg gcagcggttt attgctgtaa ataaccgcct
    51361 tcatcagatt ggcggaatag tacttctcgt ggaaatcttt cagcgcctgc tgcaccggat
    51421 taccaggttt atcgcttaaa gtttcgaggt taccaccaga aaactttgaa ccggggtgtg
    51481 ccgggttaat ggtttctgcg ctgacctgtg ccatgcgcat cccgtcacgc gtacgcgcca
    51541 tggttaattc agcgttcacc gcattacgct cacgttcggc atatttcttg tcgagcaaag
    51601 gttcagcaat agcatcggcc aggcggtcta ccgcaccagg caaggcgtcg ttctcaactt
    51661 ccagatagaa agccgtgcga tacggcgcag tgctggcatt gtgactaccg ccgtgcattt
    51721 tgagatattc ggccagactg tcagcctgcg ggtacttttt cgaccccatc agactcatat
    51781 gttcaaggta atgtgccagc ccctggtacg cctcgggatc ttccagcgac ccaacgggca
    51841 ccaccagcgc cgagagcgat ttaactgcct gcggatcaga aaccagcaag accaccatac
    51901 cgttatccag acgtatagcc tgatactggc ggttatcttt atcactttta cggatggttt
    51961 cctgaatcgg ctgccatccc gtttctgcct gacttaaggg tgcccaaagg gcaactaaca
    52021 acaataatgc tttgaaccag gtgctgcggg gcattcacgg acctcataat caacttaatt
    52081 ttctgtccag attcaacacg ttaacgcaaa tcatctgcca gaatttaatc ttgtgctgca
    52141 cgagtcagcc tatgtttata taaccatcag tccgtgactg gtgcgcatca taaagtaagc
    52201 ggatagattg cgcaattttt atacagcact catgactgat taaagcgaaa cagcggtaac
    52261 aggaaacgtt gcgactgttc aacgatggcc tccattgtct ctggtgttaa ttgccgccag
    52321 agcctttgat accagatatc atcaccttcg ccacgcacca tcatgttgcc ttcgtaagcc
    52381 tgaaggaatt tcgtacgggc tttttgcaac gtggaatcgt catccagcat ggcatcgttt
    52441 tgcgcgtcat aacaggtttt tagccacgcg ccgccacttt caggtaacac cagcaatggc
    52501 gcggacatcc cttcacgata cccctcaatc agttgtgaga ggtaatgcaa agcctgttcg
    52561 gctgcaagcg gcggaaaacg ccactcgccg tctttgcgta gaaaaaggcg actttcacca
    52621 ttaccaccgc tggcacagta gacaaggtgt tccagccaaa gttgcattcc ctgcgccaca
    52681 cttaataaag agggacgcca gcgcaacagg ccatccggct gcacctgcgg caaccagcca
    52741 gttatctgca caccgttgca ggcgagatca atttccatac tctgccccgg ctggcgacag
    52801 gcaatgactc tgtcggcaag ctgctgcatc tcctggcact gagtttccca gaaaatttca
    52861 ccaaaagcgc catacggtaa atcccctgcc gcccggaagc gacggaacaa gcgttcggca
    52921 tcatcctgct caaccagtgc attcaataac tgctgattga tttgataacg gctaagccct
    52981 tccagaataa atggctcggt gtcggggatt tcgctgtctt cagtacggaa gttcacctgc
    53041 aaacgcatct ggaagaacgc ccgcaccgga tgtgcccaga atcgttgtag cgtttccagc
    53101 ggcacggttt ccggtaaggt aaacggcagc ggctgaacaa attcagaatg tgctttacca
    53161 gcctggctgg ccgcaggtag ccattcacga gcatagcttt gtcgttcgcc tggctgatag
    53221 ttttgtggat caaacggcat tcgggtatgg aggcaagtaa gatgcgcttt tacccttgcc
    53281 tcgctttcat cacagttgag cgcttcatcg cccggtagat aatgactttg cccgatgtag
    53341 tcgatcagtt cctgcaccag taccgacggg aaacgttcac tgttatcctg aatggaacgc
    53401 ccgatatagc tgatatagag tttttgctgc gcggaaatta acgcttccag gaacagatag
    53461 cggtcgtcat cgcgacggct acggtcgcca cgcttcggtt tctggctcat caggtcaaag
    53521 cccaatggcg caagctgacg tggataaacg ccgtcgttca ttcccagcag gcaaaccact
    53581 ttgaacggaa ttgaacgcat tggcatcaga gtacaaatgt taaccggtcc ggcgagaaaa
    53641 cgctggctga tacgttcttg atccagacgc tgtgccagtt catcacgcaa tagtgacagc
    53701 ggcaccgcgt cgccatactg cgcacctaaa ccttcggcga taatcgcctg ccattgttgt
    53761 tcgatcagcg tcatcgccgc ttcggtttcc gcatccggca ggaagaaggc gctgagcata
    53821 tcgcgacaaa ccggcaacca ctcttctagc ggacgctcct gtgccagccc gcgacgccag
    53881 atgtttagct gcattagcag tgaagccaga tgccccacca gttctgcaat taagccgctc
    53941 gattcatcat aaggtagaac cgattgccac tcgccctgcg cgctctccat cgcgtagccc
    54001 agcaacatac gcgtcaggcc aaatcgccag gtgtgttgtc cggtggcggg gagttccagc
    54061 tcgcgaacgt tgtcgtcatc tatcccccaa cgaatgccgg attcgttgac ccactggcgt
    54121 aaataacgca gcccttcttc ggtgatgtca aaccgcgccg ccagcaccgg cacatccagt
    54181 aatgccagca cgtcttccga cacaaagcgg ctgtctggca acgataacag gctgataaac
    54241 gcttccagta ccggatgcga ctgccgcgcc cgacggtcgg aaatggcgta aggcaggtaa
    54301 cgatccgcag gtgcgctacc aaacacagcc cgaataaacg gactgtagct gtcgatatca
    54361 gccaccatca cgatgatgtc gcgcggagta agtgtcgggt cttcttccag catcgccagc
    54421 aggcgatcgt gcaaaacttc aacttcacgc tgcgggctat ggcaaacgtg gaaggtgata
    54481 ctgctatcca gtggatcaag cgggcgtttg ttatcgctac gggaaaactc ttcgatgttc
    54541 acaccagcaa cggcacggtt ttccagttcc agaatgtcag actgaatgtt atgcagcagg
    54601 ttatctggcg tgacatcgac aaaggcgtcc agctcctggc tgctctccag gtcagaaagg
    54661 agataaatgt agtcgcgccc aagcttaccc catgaagcca gcagcgggtt gccgacatcc
    54721 tgttcaccat cgctgttaaa gagctgcccg gcattttcgc tatcacgaaa tagcggcaat
    54781 tcgcgatctt caaaactgtg tcgacgctga cgggtcagca gtttcgccag ataagcagga
    54841 tctttaatat cgccccagta ataacggcag gggttggtaa acaggagatg gatttcaata
    54901 tgtttaccca gcgcctgtag cgcctggaga taaacaggcg gtaacgcgga aataccgcat
    54961 ataaagacgc gcgaaggtaa ccccggcggg caggtcgtcg cggactccag cgtttcgata
    55021 aagcgctgat agagattggc gcggtgccag cgcggttgcc cgagttgatg ggtatattcc
    55081 accagcgcct tccacaacgg ggcttgccag gcctgtgctt ctcccagccc ttcaaccaaa
    55141 tgtcctgttt cccactgtgc cagccagtcc ggacgataga ccagatactg gtcaaacagg
    55201 tccgccgctt ttgaggaaag ctggaacagt tttcgcttgt cgctatcgtc agtcagataa
    55261 tgccgcaaca gggtaaagtc ttcgcgctcc agcaattgcg gcagcagagt catcagtttc
    55321 cagctcatgc tctgtttgtt aaaggcgctc tctttgggga tttccggtaa cacccggacg
    55381 aacatatccc agataaagct cgctggcagc ggaaaatcaa tgtttgccgc aatactaaac
    55441 ttttgcgaca gggtcatttg cagccactgc gccataccgg tactttgcac cagaatcatc
    55501 tctggttcga aaggatcgtc cagccgttcg cgttcgacaa taaactccat caacgcttcc
    55561 agcacgtcca gacgattgga atggtagacc cttaacatag cggctcctga ctactgacga
    55621 ttcgggcaat gcaggcgggt catctcgcct tctctgcccc cgggtgaaac tagcgtaacg
    55681 ctgatgctga cacatcccgc ctgcgatgtc tgcattcggt tgacctgcca gttggcaggt
    55741 ggcgaaatcg cgcgcagttg cgtttgctgc caaccatgcc gccagagctg ttggtactgg
    55801 tttctgctgg cgagactgtt cattaatgtt cgctggatac ccgataacgc agtgacaatc
    55861 atcaccatca gcaccatcgc aaccattacc tccggcaggc taaagccttg ttgattcttc
    55921 agggaagctg acataacgcc ctctctttca gcggacaaaa atcgctccag ccgcgtggcg
    55981 aaaaaacaat gtttccatca atgacttcgc ctgttcgcca caacgaaacg ccttcataac
    56041 cggcaaccaa taaggcttca ttatctgcca gtaaacgcaa acaaacccgg gcatcggttc
    56101 cagcgtactg cgagcactga actgcgggct gcgtctgcca ggagtgcatt ttcccccacg
    56161 ccagcgccga ctgaacgatg gcctggcggc gcaatgactg actttccatg ctcacgcgag
    56221 aggcaaaact gcgatcctgc tgactcattc cttgcaatag caagctaccc aaaaccagca
    56281 gcatcaggac cagagccagt gacgaaacac ctttttcgcg gttcacaggt tgaatcctgt
    56341 cacgctatag ctggcatcca ccacggtttg cggttcagac ttactggcag cacgcatatt
    56401 aaccgtcaac accggcgaga agccgctgac atcctgacgt acgacctgaa aagtgtcgat
    56461 aatgatggca tccggattag tgactttatc ccagccctta ccttcacagg atgtcgcacc
    56521 gcgtagcgtt tccagcacat gctccttcag acgaaatcca atctggtcag actcttttac
    56581 cggttcgcga tcccagatac cgttactgtt cgcatcccac tgcacaatga tacagtcacc
    56641 ctgtccgaca atttccagcc cttcgccggt acagctgcca tgacaataac ccgccctctg
    56701 gagatgcttc gcgacggtaa atacccgcag ccagatttca tcttccagcg ccagcttacg
    56761 ggtgctcgtt aaactttcac gctgtaacgc aggcagaaag cgtgcagccc ccagcaacaa
    56821 tacgctactg atcgccatag caatcaacac ttccagcaga gaaaaacctt gctcttttac
    56881 aggcatcctt ctgtttctcc ttgctgacaa agccggagtc ttccccacgg cgaaaccacc
    56941 agccaccact cgcccgttga gtttttgaag cgaatatgcc cggcccatgc ggtattgcgc
    57001 aggccaaaga aagcaagcga aggtgtcagg tcgctcattt cgacttcggg ccagcgtggc
    57061 acaaagacca atggtgaact gccatgacag gtattggccc cagcagcgga actcacaagg
    57121 caccataacg tcccctccct gataacgctg atactgtggt cgcggttatg ccagttggca
    57181 tcttcacgta aatagagcaa atagtcccgc gcctggctgg cggtttgcca tagccgttgc
    57241 gactgctgcc agtattgcca gccatagagt ccacttgcgc ttagcatgac caaaatcagc
    57301 atcgcgacca gcgtttcaat cagcgtataa ccacgttgtg ttttcatgcc ggcagtatgg
    57361 agcgaggaga aaaaaagacg agggccagtt tctatttctt cggcgcatct tccggactat
    57421 ttacgccgtt gcaggacgtt gcaaaatttc gggaaggcgt ctcgaagaat ttaacggagg
    57481 gtaaaaaaaa ccgacgcaca ctggcgtcgg ctctggcagg atgtttcgta attagatagc
    57541 caccggcgct ttaatgcccg gatgtggatc gtagccttca atctcaaagt cttcgaaacg
    57601 gtagtcgaag atggattcgg gtttacgttt gataatcaac ttcggcagcg gacgcggttc
    57661 gcggcttaat tgcagatgag tttgatccat atgattgctg tacaggtgcg tatcgccacc
    57721 ggtccagaca aaatcaccca cttccagatc gcactgctgc gccatcatat gcaccagtaa
    57781 cgcgtagctg gcaatgttga acggcaggcc gaggaagacg tcacaggagc gctgataaag
    57841 ctggcaagag agtttgccgt ctgccacata gaactggaag aatgcatggc acggtgccag
    57901 cgccatttta tccagttcgc ctacgttcca cgctgaaaca ataatgcggc gggaatccgg
    57961 gtcgtttttc agctggttca gtaccgtagt gatctggtca atatgacgac catctggcgt
    58021 tggccaggcg cgccactgtt taccatacac tggtccgagg tcgccgtttt catcggccca
    58081 ttcgtcccag atggtgacat tgttttcgtg tagataagca atgttagtgt cgccctgcag
    58141 aaaccacagc agttcgtgga tgatggaacg caggtggcaa cgtttagttg tcaccagcgg
    58201 gaatccatcc tgcaggttaa aacgcatctg atgaccaaaa atggaaagcg ttccggttcc
    58261 ggtacggtcg tttttctgtg tgccttcgtc gagcactttt tgcatcagtt ctaaatactg
    58321 tttcatggtt cctcaggaaa cgtgttgctg tgggctgcga cgatatgccc agaccatcat
    58381 gatcacaccc gcgacaatca tcgggatgga aagaatttgc cccatgctga tgtactgcac
    58441 ccaggcaccg gtaaactgcg cgtcgggctg gcggaaaaac tcaacaatga tgcgaaacgc
    58501 gccgtagcca atcaggaaca agccagagac agctcccatt gggcgcggtt tacgaatata
    58561 caggttgagg ataataaaca gcaccacacc ttccagcagc agctcgtaaa gctgtgatgg
    58621 gtggcgcggc agcacaccgt aagtgtcgaa aatggattgc cactgcgggt tggtttgcag
    58681 cagcaaaata tcttctgtac gggagcctgg gaacagcatg gcaaacggga agttcgggtc
    58741 aacgcggccc cacaattcac cgttaataaa gttgcccaga cgcccggcac caagaccaaa
    58801 cggaatgagt ggtgcgataa aatcagaaac ttggaagaag gaacgtttag tacggcgggc
    58861 aaagataatc atcaccacga taacgccaat caggccgccg tggaaagaca tgccgccgtc
    58921 ccagacacgg aacagataca gcggatcggc cataaactgc gggaaattgt agaacagaac
    58981 ataaccaata cgtcccccga ggaagacgcc gaggaagccc gcatagagta agttttcaac
    59041 ttcatttttg gtccagccgc tgcccggacg attcgcccgt cgtgttgcca gccacattgc
    59101 aaaaatgaaa cccaccagat acatcaggcc gtaccagtga agcgccacgg gtcctattga
    59161 gaaaatgacc ggatcaaact ccggaaaatg cagatagcta ctggtcatct gtcaccacaa
    59221 gttcttgtta tttcgctgaa agagaacagc gattgaaatg cgcgccgcag gtttcaggcg
    59281 ctccaaaggt gcgaataata gcacaagggg acctggctgg ttgccggata ccgttaaaag
    59341 atatgtatat gatccgcgct ataaccctcc gcgaatcagc ccgcccatgc cgcgacgctc
    59401 cataaaggct gcaacctgat ggcgaacttc ggtcgccagt tgcgcttcca gactacgctg
    59461 cgcaagattt tctgcttcgg cataatcaat gcgccgcagc aggtatttta cccgcgctac
    59521 agaacgtccg ttcatagaca gatggcgata cccaagccca atgaggattg ccacgcacat
    59581 gggatcgccc gccatttcac cgcacaaacg gagatcgatt ccatgtattt ccgcttcccg
    59641 ggcgatcatc gccagagctc gtaacattgc aggatgaaga ctgtcataaa tgttcgccac
    59701 ccgggtattg ttgcgatcaa cggccagaat gtattgagtc agatcgttgg tgccaacaga
    59761 gatgaaatcg acccgctttg ccagatgcgg cagcataaat accattgacg gcacttccag
    59821 catgatgccg atacgtggtt tgggaatttc gtaaccgatc atctcctcga cttcacgtcc
    59881 ggcacgttca atcaggcggc gtgcttcatc aacttcatcg aggcttgtga ccatcggcaa
    59941 cagaatattc aggttgcccg tagcggcatt agcacgcaac atcgcccgca cctggatcaa
    60001 gaagatctcc ggctgatcga gcgtaatgcg aatcccacgc caacccaggc atggattctc
    60061 ttcgctgatc ggcatgtaag gcagctgctt atctgctccg acatccagcg tacgcaaggt
    60121 gacgggttta tcattgaaca tttgcagcat cccctgatac tgcgccacct gttcttcttc
    60181 cgacgggaaa ccactttgca gcatgaatgg gatttcagtg cgataaagac cgatgccatc
    60241 aatacggctg cccagttttt cttcatgttc cgggcttaaa ccagcattga gcatgacttt
    60301 tatacgctca ccgcttttta actgcgcggg taaattgacg tcatcttccg ccagacggct
    60361 aagctcaatc tcttcactaa ttagccgctg atattcttgc agcagtaccg gctccggatc
    60421 gaccagcaat tcaccgcgat agccatcaac gatcagcgtc ctacgatgca gcaccgaagg
    60481 ctgaatatcc gcgcccatca cggtagggat ccccagcgca cgtaccatga tcgcagcatg
    60541 ggagttggct gcgccatctc gcacgacaac accgactaag cgatcctggg gcagctcagc
    60601 aagagttgtc gctgacagtt catctgccac cagaatgaaa cgttccggcc aggcgttcgg
    60661 cccttgatta gcgtcatcaa gatgaaacag caatcgctga cccagcgcac gtaaatcgcc
    60721 agcccgctct ttgagatagt tatcgcttag cgcggcaaac tgttcggcaa atttttcaat
    60781 gaccgttttt accgcccact ctgccaccga gcctttatca acctcggcaa acaattcgcg
    60841 acgcagccgg gtgtccgaaa gtaggtgcga gtaaagatcg aaaatagccg ccgtttcttt
    60901 ttgtgctccg gcggcaaagc gtttgctgta gcggcgaaac tcgtttgccg cttcttccag
    60961 cgccccggtc agtcgttcgc gttccagagc cggatccagc gttgatgcct gatacacctg
    61021 ttccattaaa ggtaacgtgg catcctgcca gccttcggca atcgccacac caggtgctgc
    61081 cggtaatgcg cggattcgcg tctggcgata ttgcccaaac aaggcagtca actgcgactg
    61141 agaaagaata gctgccatct gggtggcaag cgtcaccagg aaggattctt cactttcgtc
    61201 atactggcgc aactctcgtt gctgtaccac cagtacacca agcaactggc gacgttgaat
    61261 aattggtacg cctaaaaacg cgcggaaacg ttcttctttt acggagggga tgtatttgaa
    61321 gctggggtgc ttttgcgcat ctgcaaggtt tatcggttcc gccagcctgc caaccaggcc
    61381 gacgatccct tcatcaaacg cgagcgttac agtgcgaccg cgtggttttt tcagcccccg
    61441 ggtcgccatc aggtagtaac aacgtcgatc atgatcggcc aggtagaccg aacagacctc
    61501 ggtatccatc gcaagacaga tgtcggtaac cagaatattt aacgcctcat tcaggcgtgg
    61561 tgcgctggct accttttcga ctatttcgcg caggcgagtg agcataattg gcgtgactta
    61621 acctctttta cgtcgataag cagatgtgtt ttgtggtttt ggcgtatttt cctgcagtga
    61681 catcaccaca ctcgcgaact ctttcattac cctacggtag acatcacgtt taaatgacac
    61741 cacctgtctg accggatacc agtaacttac ccatcgccag ccgtcaaact ctggtgtact
    61801 gctggtttgc atattgattt ctgcatcgcc gctcaccagc tgcaagagaa accatttttg
    61861 tttttggccg atacaaaccg gcttcgtgtc ccaacgcacc aaacgtttcg gtaatttgta
    61921 gcgcaaccag ttacgcgttg aagcaaggat tcgaacgtct ttgcggctta atcctacttc
    61981 ttcaaacaat tcacggtaca tcgcctgctc tgcggattct ccggggttga ttccgccttg
    62041 cggaaattgc caggagtgct gaccaaatcg ccgggcccac attacctgcc cctggcgatt
    62101 acaaatcacg atacctacgt ttgggcggta gccatcgtca tcaatcaccg gactacctca
    62161 aaataaagct ttatatacga atgattgttt catactccag gaagacggta aaccactctc
    62221 tgcagggcat tacacactaa taacaattga ataactcaca gttatgtgca gagttataaa
    62281 cagaggaagg ggtggatagc cgtttttatt caccttttct gtggatagag ttgtgaagaa
    62341 ctacggaatt actacgggaa aacccggagc attctgaata agcccgatat agacatttta
    62401 aaatattcat tattagtcaa tgcattacat gtaatttgag taatgaaaaa gagtaattcg
    62461 tgacccaggt cacacctctc atttacgggt tggcgaaaga tcaaccaatg ccgtatttat
    62521 ccacagaatg tgccactaag ttaagcactg aaccactaaa aactggagtt tcgtcgcacg
    62581 tcaaggctgt aaatggaaac agtagtggag gtttttcaca gttatcccag ctttctgtgg
    62641 ataacatggt gtaagatcct gtttattttc agtgaccaga tttggaaaac ctattgcagt
    62701 gttgcgcaac tcgattaccg gcagcctaaa aagcaatata aatcagagaa ttgaacaaca
    62761 catgtggaaa aagttacact gcgaatattc ggcacataat tgctgtttgt tttttaacca
    62821 aggtatcatg acatgtccca acctcgccca ctcctctctc ctcccgaaac tgaagaacag
    62881 ttgttagcgc aagcacagca actttctggt tatacattgg gagaactggc ggcacttgcc
    62941 gggctggtta cgccagagaa tttaaaacgc gataagggct ggattggcgt gttactggag
    63001 atctggctag gtgccagcgc agggagtaaa cctgagcaag attttgctgc tctgggcgtg
    63061 gaacttaaaa ctatccctgt ggatagtctt ggtcgtccgc tggaaacaac attcgtttgt
    63121 gttgccccgt taacgggtaa tagcggggtg acctgggaaa ccagccatgt gcgccacaag
    63181 ctcaaacgcg tactgtggat accggttgaa ggtgagcgca gcatcccgct gacgcagcgt
    63241 cgcgtaggat caccgttgct gtggagcccg aatgaagagg aagaccgaca gctacgcgaa
    63301 gactgggaag aattaatgga tatgattgtt ctcggtcagg ttgagcggat caccgctcgt
    63361 cacggggagt atttacagat acgaccgaaa gcagcgaatg cgaaagcgct taccgaagcc
    63421 attggtgccc ggggcgaacg gattctgacg ctgccacgcg gcttttattt gaagaagaat
    63481 ttcaccagtg cactactggc ccgtcatttt ctgatccagt agccatcgct ttgacctgcc
    63541 gctttccggg catataatta ccgcttcatt tttttggcag ggctttttag atgttatttg
    63601 catggataac cgatcctaac gcctggcttg cgctcggtac gctgacgctg ctggagatcg
    63661 ttcttgggat cgacaatatt attttccttt ctctggtggt ggcaaagctc cccacagcac
    63721 aacgtgctca tgcgcgccgt ctggggttgg cgggagccat ggttatgcgt ctggcgctgc
    63781 tggcatcaat cgcctgggtt acgcgcctga caaatccgct ttttacaata ttcagtcagg
    63841 aaatttccgc ccgtgatttg attctgcttc tgggtggctt gttccttatc tggaaagcca
    63901 gcaaggaaat ccacgaatcc attgaaggtg aagaagaagg gctgaaaaca cgcgtttcat
    63961 cattcctcgg cgctatcgtg cagattatgc tgctggatat tatcttcagc ctcgactcgg
    64021 tgattaccgc tgtgggtctg tcagatcacc tgtttattat gatggccgcc gtggtgattg
    64081 ccgtaggcgt gatgatgttc gccgcgcgct cgattggtga ttttgtcgaa cgccatcctt
    64141 cggtaaaaat gctggcgctc tctttcctga ttctggtcgg ctttaccctg attctggaaa
    64201 gtttcgacat ccacgtaccg aaaggttaca tctacttcgc gatgttcttc tctattgcgg
    64261 ttgaaagcct caacctgatt cgcaacaaaa agaacccgct ctgatactcc gtacgctctc
    64321 ctgcgggagg gcgtactcac tcctgttacc ctcacagttt aagattttcc tgctttcaag
    64381 atatataacg tcggtttata aacagactat tatcataggt gggccaaatg aggataaccg
    64441 gatgaaaaaa tgggcagtaa taatttctgc agtcggactg gcgtttgctg tttccgggtg
    64501 ttccagtgat tacgtcatgg cgaccaaaga tggccgtatg attttgaccg atggaaaacc
    64561 tgaaattgat gatgataccg ggctggtgag ttatcacgat cagcaaggta acgcgatgca
    64621 aattaaccgt gatgatgttt cgcaaattat tgaacgttaa caaataaggt cagcatc
//
LOCUS       DAKISA010000010        60757 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.010, whole genome shotgun sequence.
ACCESSION   DAKISA010000010 DAKISA010000000
VERSION     DAKISA010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60757
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.010"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..460)
                     /locus_tag="OVW45_000659"
     CDS             complement(<1..460)
                     /locus_tag="OVW45_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCV5525182.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARR"
     gene            complement(538..1287)
                     /gene="btuD"
                     /locus_tag="OVW45_000660"
     CDS             complement(538..1287)
                     /gene="btuD"
                     /locus_tag="OVW45_000660"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCV5525183.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            complement(1287..1838)
                     /gene="btuE"
                     /locus_tag="OVW45_000661"
     CDS             complement(1287..1838)
                     /gene="btuE"
                     /locus_tag="OVW45_000661"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCV5525184.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            complement(1901..2881)
                     /gene="btuC"
                     /locus_tag="OVW45_000662"
     CDS             complement(1901..2881)
                     /gene="btuC"
                     /locus_tag="OVW45_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCV5525185.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASAVLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            complement(3074..3511)
                     /locus_tag="OVW45_000663"
     CDS             complement(3074..3511)
                     /locus_tag="OVW45_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001434769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1640 domain-containing protein"
                     /protein_id="HCV5525186.1"
                     /translation="MSDKLERRIERLEGDLSLTRNDLATLAERTTNLSTKADVGEVKG
                     ELKADIAHLKGALECDIANLKGELKSDTAHLKEQLKSDINSLKGELTEAMDKRFDKIM
                     DEMNRRFDKVDDNTKWRWSGIIVPVCTTIFTAAVAVFVAKFVG"
     gene            complement(3612..4112)
                     /locus_tag="OVW45_000664"
     CDS             complement(3612..4112)
                     /locus_tag="OVW45_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525187.1"
                     /translation="MEIKKPTKKELYDYLLSKYIEDKCKEEADEINKKSMSRVKKHKE
                     RLMEITPEIFFRFLSEKGVSSVCPSCGSSRLSVPESMDLCWDKNKKPENFNNLPLEEQ
                     SELIKENIKHYVSYTFLGDVKSIPDMRKTYYTLHCLNCGYLSLYRTSAVLKWLEKDKA
                     QDGDNG"
     gene            complement(4115..5053)
                     /locus_tag="OVW45_000665"
     CDS             complement(4115..5053)
                     /locus_tag="OVW45_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCV5525188.1"
                     /translation="MRPEGRKGKRIRKKFKTKSDAVLYERWVLAQQHNNEWKGNSIDR
                     RPLSVLIDLWWKYHGQLMRSGHNTHLKLLRLSEAMDDPCVHKLNTTMLTELRVYRIEQ
                     GIQPSTINREIGALSAMFTALISSGHFLNDNPVQGLKGMKVNEREMGYLSKSECVQLL
                     DALAENPDERLAVEILLSTGARWGEVEALEQRRVLHCRITFSKTKNSKNRTVPISESL
                     FEKIKKRGGKLVFPTLDYPLVRDVIKTVAPDIPDGQAVHALRHTFASHFMMNGGNILT
                     LQKILGHAKIQTTMIYAHLAPDYLQDAVRFNPLGGM"
     gene            complement(5142..5453)
                     /locus_tag="OVW45_000666"
     CDS             complement(5142..5453)
                     /locus_tag="OVW45_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCV5525189.1"
                     /translation="MSSGYEKKLKEIRKSEGLTQVEFADVTGINLGTIKNYESGKREV
                     GLSVVDRVINSKDFEKYTMWLMTGKTNEAAGQISPALSPDGYDVTSRSQSPRKTGTQP
                     G"
     gene            5546..5824
                     /locus_tag="OVW45_000667"
     CDS             5546..5824
                     /locus_tag="OVW45_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCV5525190.1"
                     /translation="MAEVLNTYEQEDIERLAAFYPYRDEHGLPILEESLKDYAKRTNQ
                     AVNTVKRQADRGSIPLTPGKKNSRRTVNLFAIFLKTIRSAEKYVQMTK"
     gene            5839..6177
                     /locus_tag="OVW45_000668"
     CDS             5839..6177
                     /locus_tag="OVW45_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001698482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HCV5525191.1"
                     /translation="MLKQRRNFRTETERQANRFATSASRSNIRYSLSETHATPDGHTV
                     KQIGEHTWMIEKAGIVVHKYPRNPFTGNRIFALSCGDNQFGQDFTLYEALRTVDRLLY
                     GENFIKQAEL"
     gene            6219..6461
                     /locus_tag="OVW45_000669"
     CDS             6219..6461
                     /locus_tag="OVW45_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525192.1"
                     /translation="MRKHTAEQVNEFLQGYYFDNEINPRARKTHFEVMKCGIFSVRNT
                     LFCSKDTGASEDLKELNRMAKQLTDGMVPEPAIITE"
     gene            6467..6682
                     /locus_tag="OVW45_000670"
     CDS             6467..6682
                     /locus_tag="OVW45_000670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525193.1"
                     /translation="MNFSYSFSVPESLFVITLIAAAMFFAICILTLIDTWREIRDRRR
                     QKKKRDAYNAFIQHKEDVERKARRLFS"
     gene            6750..7172
                     /locus_tag="OVW45_000671"
     CDS             6750..7172
                     /locus_tag="OVW45_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525194.1"
                     /translation="MINPTLTLKDKALAGAMFLRNYAAMMADTDNPMMVLSVEPHLID
                     ADAIEQVVQENTCLRNQLATLQTAADPAKESAEHTCHTTFIKGTRVCLKNSPQQRGTV
                     VDTRTSSPAIDRVFVRFDSPFEEDRWVKTRNLEITPDK"
     gene            7410..7940
                     /locus_tag="OVW45_000672"
     CDS             7410..7940
                     /locus_tag="OVW45_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525195.1"
                     /translation="MLQMKFKPCFIDAFRNGRKTTSLRPTGFDCYQAGQESAEYFHED
                     TLTRNIVLPDYSAGGTLVFEEGTTFTRASDFAGLLKRQPYRQYDNIHLVTENKNGETV
                     PFAVAFIADIGVIQGRQITDEAAVRDGFNPENHPRDELFAFMRDTCHIENPKNETYWR
                     YTFTHIVMLSQWRADA"
     gene            7937..8176
                     /locus_tag="OVW45_000673"
     CDS             7937..8176
                     /locus_tag="OVW45_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525196.1"
                     /translation="MKPAIICILCESTVTGDHYRIRERHINPDRSLLDNITGPDDERV
                     ICLNDGSQLRVRNIRREITLEPTPFTRGKKPESDA"
     gene            8173..9135
                     /locus_tag="OVW45_000674"
     CDS             8173..9135
                     /locus_tag="OVW45_000674"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HCV5525197.1"
                     /translation="MKGILNQRSPLKWAGGKFSIMPQLREYLPKAACLIEPFVGGGSV
                     FMNTDYDHYVLCDSNPALINFYQQLTTNTMELMDRAWSLFKDGGTPEAYKRHRGAFNT
                     IALATDRLRGESLEWAALFLYLNRYCFNGLHRTNQKGEFNVPFGKHRTPYFPDQEMRL
                     FADKARETRTRFIHADFRTAFTMPAEIRRFSSVHGGIAIYCDPPYLPPDGKEAFTHYN
                     GRAFTRKDHRDLAALMMNAWQRYGIRPVVSNSDTTETRQIYSSFELHTLSVRRSVAAS
                     SSARQQAKEVIGVYPPAHKSQLRDVNVGWPSPLHAPHPTTEIMQ"
     gene            9132..9443
                     /locus_tag="OVW45_000675"
     CDS             9132..9443
                     /locus_tag="OVW45_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HCV5525198.1"
                     /translation="MTVIYIAGPMTGKPDFNRTAFTMTATRLKMQGHTVLNPATLPDG
                     LRYRDYMLIGSAMLHCADVIYLLDGWEDSPGAKEEHATALKLNLIISTPESRKEAKSC
                     F"
     gene            9434..9988
                     /locus_tag="OVW45_000676"
     CDS             9434..9988
                     /locus_tag="OVW45_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCV5525199.1"
                     /translation="MFLKPMGGPGKAPKHVSAWTQQEDELLISLYQDHSTWQMAERLQ
                     RTRSAVMHRILFLRNRGLIGRKKKAPLSAEAIAFLIKNRHAKTARELARKAGCSECTV
                     RYTLHKRGYSLKKCGESHHCTRYSDRLAELVTELRDRRNMTFCIIAKHINITMQMHIS
                     DDTAFHLYNRRTAADALLYELLPN"
     gene            9998..10615
                     /locus_tag="OVW45_000677"
     CDS             9998..10615
                     /locus_tag="OVW45_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HCV5525200.1"
                     /translation="MHTQKNRLPCRNQSGYISAAPHKTGAGFRSPLTNIAHNRASGFF
                     VRTVSPHLFRTRIMVGRTGPTSVGPGSMFAGTANLVRLATHSFAALDGEFSKLTHIEA
                     TSWQTANNSAHTLRVVTSGLKSTADFSAHHASRKSCTSICCMNAATRCQTATAPLYSA
                     IWRMICTSFNSSSSSKTNSINSCSGPFLHLAAGGLRTSLTKRGAA"
     gene            10612..11001
                     /locus_tag="OVW45_000678"
     CDS             10612..11001
                     /locus_tag="OVW45_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HCV5525201.1"
                     /translation="MIDRHDFVKWVRTQDTRLAPKLQAMFDFYLRARASQARTTKPEN
                     AGPLYFIVDDCYRVDFTPHGLALYTLTPHGDSLLAYYNSPASVFAAMLAHRTAGGCAS
                     LSEYTTEFNRLSALFSQEWQRVTGCQP"
     gene            10998..13490
                     /locus_tag="OVW45_000679"
     CDS             10998..13490
                     /locus_tag="OVW45_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HCV5525202.1"
                     /translation="MSEFTWDWNEPRQAIDPATFAKYQPTPETPLQQAIRYYQEADLK
                     AQEVREAEEEAFFVQSDMGKKLMVALDEDERREKVTQSIISKRQATEQDPVARAFVTL
                     KALPVYLREPLSRRLSFLRKKQESDRQKGKKSRPSEGYARGTLRKIFERLDRTDSRWL
                     TPGYRALAGRERLDDLLYLPQLNKHQIQTLGTMTAAMFSSSFETLCDGFGARDGELTM
                     EVALKAYQMLARMALHLHAMPPHYDALTTDKDRKNEPDTELLPGAILRLTCADWWKRK
                     LWLLRCEWREEQLRAACLVSRKTSPYLSQDALSEFRAQREKTRDFLKSFMLENEDGFT
                     IDLETVYYAGVSNPVHRKAEMMATMKGAELIAESRGDKAVFLTVTCPSKYHATTENGH
                     PNPKWNGATMRDSSDYLVNTFFKAVRNRLDRKGLRWYGIRTVEPHHDGTVHWHMMVFA
                     HPEEIDSIVTIARDIAIREDRHELGDDITPRFKAEYVDGSKGTPTSYIATYIGKNLDS
                     HAVDGIDPKTGKPRIDDESGKTMAESVERAIGWARLHRVRQFQFFGIPSRQVWRELRR
                     LASQMARNPEGPQRLKDDAMDAVLAAADAGCFATYIEKQGGVLVPRKDYLIRTAYDLA
                     DELNDYGEQSVQIYGIWSPLIGESSRVCTHPDNWKLVRRKPEAEDSARENGFDLQGGP
                     AAPWTRGNNCPGDEKTAGTGTENRQRGSRLTLPESGLAHWLQTLTRHERKQLIRQLKN
                     QPPTTDTGQNNTRPASDTPVRVEVVVPVDTTTIAQITRELDALGVQLPDAAIISVSNG
                     ARVRPGDGRIAYWSATTRRIVITQESSAEVRS"
     gene            13487..13918
                     /locus_tag="OVW45_000680"
     CDS             13487..13918
                     /locus_tag="OVW45_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525203.1"
                     /translation="MTRFHCTMQRQQFDAVMRQRTPAMTLRGVATRPQPGDELEITVA
                     SPDRTTTTTITDILNRRPGSEYQSWTIAVRPRRSPEEPEPLTPAGKIMQKYHEEQARK
                     HNVALARTLTAVNRKAALARRVFSSQHPSFARPAQHRRDPQ"
     gene            13915..14163
                     /locus_tag="OVW45_000681"
     CDS             13915..14163
                     /locus_tag="OVW45_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525204.1"
                     /translation="MREQLPDTVPVQEIKENIRQQLHGFYITYDLWLKNGANPDGIFS
                     RNCGLCASLWDYLELTGADKEAALELLHIDFRNAGRTA"
     gene            complement(14219..14494)
                     /locus_tag="OVW45_000682"
     CDS             complement(14219..14494)
                     /locus_tag="OVW45_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HCV5525205.1"
                     /translation="MAGVMKIECPACHCRAAIRKTAWQDDAKTLAVVYCTCTNSDCNM
                     RFSLNLSDLRVISPSDLQTDGVVKALLQRLKPDERQMALDILLSDGA"
     gene            complement(14545..15600)
                     /locus_tag="OVW45_000683"
     CDS             complement(14545..15600)
                     /locus_tag="OVW45_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000044295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCV5525206.1"
                     /translation="MSKRKSARLRKRAQTENFTPGRGNIITFGEPEPILTTGTEYINV
                     WYNGSYNYWTLPIDRLALAQLPNLNAQHGGVLYARKNMVCASYQGGGLTTDQMEMAVF
                     DYLLFGDVAILKVRNGWGRVVALAPLPSLYLRRSKQLDFIVLQEGEPLVYSPSDVVFL
                     RMHDPRQQIYGLPDYIGGIHSALLNSEATIFRRRYYHNGAHMGFIMYASDPNMSVEAE
                     EAIRKKIESGNGLGNFRNMFISIPKGQPDGIKIIPVGDIQQKDEFSNVKNISAQDVLT
                     AHRFPAGLAGIIPQNASGLGDPDKARTTYGRDEVTPVCRKFMQTVNSDPEIPPSLHLN
                     FTLEDVENVVSALADEK"
     gene            complement(15597..17420)
                     /locus_tag="OVW45_000684"
     CDS             complement(15597..17420)
                     /locus_tag="OVW45_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HCV5525207.1"
                     /translation="MKYTEEVRDAARALYLKFSTPKEIAATLGLPVRTVYNWAVRGQW
                     NEMLPAESVEVAVARRVERLTCKDGKSELELEELRFLISQHVRLMAQKNKHAERMEEI
                     RAMVNTGVVVNDGRQGSSEEGNDEGGKKRKRLRKNDVGGLTKEIFDEKANGHLFEYQQ
                     YVREHGDELFRFILKGRQEGFTYYFAWEGFEKAVLTGKNQIFFSASKPQAEVFRFYIL
                     SIAQQFFGVELKGNPIRLSNGAILRFLATNPNTAQSYSGDLYGDEVFWIPKFARLHEV
                     ASAMATHDEFRITYFSTPSAKTHQAYRLWTGDEWKGDDPKRKAAEFPTSKELRKGGQV
                     CPDGIWRYVITMEDACAKGLSARVNIEKLRNRYSATAFAMLYMCEFTDSRDTVFKFSD
                     LEKCEVEFGIWQDFDPSALRPFGNREVWGGFDPSRTGDNSTFVIVAPPVEPEEKFRVL
                     AVYQWVGLNFTWQVKQIEELMKRYRFTHIGVDITGIGRGVYDQLVRSAPREVMGINYS
                     VDNKNKLVLKMIDLIERRRIQWVKDAVDEVTKERADIPLAFMAIRRVMTASQNAMTFA
                     AERSETTGHADVFFAISHAVCNEPLDYEYDRPSVWAFGKAA"
     gene            17518..18690
                     /locus_tag="OVW45_000685"
     CDS             17518..18690
                     /locus_tag="OVW45_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HCV5525208.1"
                     /translation="MKPAKTAAIIPPDTSKSGNEMAKQKYTTDWICIATEGYAIDGRP
                     ITREMIQQAADNYSTARYTAMIWPNHPVYSLQEREFTPNLGLVSELAAKEDAGRLRLM
                     AKLEPNQALVNLNDRGQKLFTSCEFWENYANTGQTYLYGLAATDSPASLGTQRMEIKS
                     VENNTQGSRPQYAAAGNIEMFSLGRLTLVADTPTNHHEDKSMTKEQANQLLAQIVTLT
                     AKIDAQANTQPQAQQPAPAQPTASATADTQPVTGDFSAHANHVAELSEKLSVATDTLA
                     INPKDDTARQNYTTAVTELQTALNTFTVQPQDAETAKLQARIAARNGGQQPAPAAAQN
                     FSTRKTEKDDEADAATLTTLTSAIENLSQRFSVIESQRTPTPGAAPGGQPAQFEPL"
     gene            18738..19787
                     /locus_tag="OVW45_000686"
     CDS             18738..19787
                     /locus_tag="OVW45_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HCV5525209.1"
                     /translation="MAAQMLSPQAQKFLRNYAISMAKANGVFTAQQFFTISPPRETLL
                     RDALIENADPFMGKITVMLVEQLVGQVVSTGIPGLYTGRKKDGRFNKALGSEGNEYKL
                     YEVDSGSFLDYTTLTNWANAGTENEFYNRLQAFFYKSVTNDLLRVALNGTHAADETNP
                     DTCPNGEDIHPGWHQIVKARTPKQIITDKVVLNRTGTGADFTSVDAIAADVINTCIPP
                     EFRQHPDLVVLVSQNIIAADTVSMLNRIDRPTEKVAAQLINREIAGRKSFSPPFMPDN
                     RLVVTTLWNLHMYFQRGTQQRRAEWIDDRKRFENNWLRMQGCAVEYDALYGSFDNIEL
                     AGLPSPEHAKPNQVA"
     gene            19799..20500
                     /locus_tag="OVW45_000687"
     CDS             19799..20500
                     /locus_tag="OVW45_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCV5525210.1"
                     /translation="MQTPAQKFRARLAAQQAMERHEALTVRDSLHLQLAQLEIDCRAV
                     SSCATNADRVAFKRESLLPRWMPTIEAWLQSGEEYKYIPFTWCIVWLFDTGEMDKALE
                     WADIAIGQNQPMPPRFDRDLPTFVADEVMQWAQIQFDKGLSVEPYFSRVMSHIEKDWT
                     LHEEIVARWYKFAGLNLLRDSDGKPTTPAAIYDVAKLRSADEWMARAHSTTPKAGVRA
                     IRNKIAARLRALRAV"
     gene            20659..21075
                     /locus_tag="OVW45_000688"
     CDS             20659..21075
                     /locus_tag="OVW45_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HCV5525211.1"
                     /translation="MLKNDGFWPDLVMADFRRSRTIPLDIPASLAEQALLSAVFEVNT
                     ALTGVKSRYVVQGIAQAADVEGPQLNGESALCAQYKKAVFARAKADLLGEFTAVNTRH
                     ADSNIDSNDTRETLLAEAAFVLRAIQGAGRVGVYIL"
     gene            21072..21551
                     /locus_tag="OVW45_000689"
     CDS             21072..21551
                     /locus_tag="OVW45_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000077226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCV5525212.1"
                     /translation="MSQFDSLYAYLRQVFDISVFDNTGFDSWMSDIRLLPAEKDMGLG
                     QIRMGIRQYNAVFDWQRWPWRQVDPDLLLCALLVWRDEQANDYYSQLELDAPDVNIEP
                     TDDNIAEVTVSMPLADPIILIPDEAGFIMMGGIRYRLDKPEIWVAENITVSTIRAGK"
     gene            21555..22283
                     /locus_tag="OVW45_000690"
     CDS             21555..22283
                     /locus_tag="OVW45_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525213.1"
                     /translation="MDISLHGELNRTQLKQLQTMLANGELPPAKQQRLLQRIAKRGVI
                     PAAKRHVREQKNVDGSSFAERRYGNRPLLKGLPKFLKVHDMPSAKSVRIYAAGRSYRQ
                     PHNHREISVGAVGYIQSHGVTFTVNASQLKTDAMEKASKEPCSRRQAIKLRKLGYTVS
                     GKKRGTRRKPSTADIRTSLTKGQAGVIIRSMEGIPSKSCWTITIPPRQFLGVSNEEFA
                     RILSRQLQGINYGWQTKPQDIKRK"
     gene            22287..23411
                     /locus_tag="OVW45_000691"
     CDS             22287..23411
                     /locus_tag="OVW45_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2586 family protein"
                     /protein_id="HCV5525214.1"
                     /translation="MTFPQVTINQLNTHSGSKRGIACTLLMVGEHTKAILPTPVTAQT
                     DLDSLLGTKDSLLRSNVQAFLDNAGQNAMIWLATVQTPQPSGQTRTWADVVMDAQATL
                     SVEGVVVVRANASGNDIDKAQQLRSELNNTFQRWTWFILAVRGCGTGEKWSEYVTVMT
                     GLQKGIAAYAVQLTPMLFGNEPGILAGRLCNPSVTIADSPARVATGALVNMGRNDKPQ
                     DSDKRELDIATIRALNRARFSVPTWYPDYEGYYWADGVTLDVDGGDYQAIEYLRVADE
                     MARQVRLLAIPKIANRSLNSTPASVAAHQQLFAKPMRDGAKSLKINGTVFPGLCMSPH
                     DGDVKISWPEKDKVQIAIVVRPYNCPKEITISIMLDESGE"
     gene            23415..23876
                     /locus_tag="OVW45_000692"
     CDS             23415..23876
                     /locus_tag="OVW45_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2597 family protein"
                     /protein_id="HCV5525215.1"
                     /translation="MNTERISGMSFDTSFNGRIVHVKTATLDITDNTKAIQERGVPDG
                     WVRGDVEAGGEIELDTVNFQLLGEAAREAGSWRDIEEADFLFFARAAKTELRVEAFGC
                     KLLISNLLNIDSKGGDNISHKIKFMVTSPEFVKIDGVPVLSATDVRDIMNQ"
     gene            23885..24223
                     /locus_tag="OVW45_000693"
     CDS             23885..24223
                     /locus_tag="OVW45_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001192103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HCV5525216.1"
                     /translation="MQSTPHNWSDWLAIIKDWLRGDIPLDSLLMTAVIAALRVFYTGR
                     SWRRLLLEVPLCCLLAVAAFTLIKPVPVAWLTEDWRVGIGAAIGLIGVEHIRALGVFI
                     TKKFAGKSDE"
     gene            24220..24663
                     /locus_tag="OVW45_000694"
     CDS             24220..24663
                     /locus_tag="OVW45_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCV5525217.1"
                     /translation="MKISDNGLAALKREENCKLTAYPDPRGVWTIGTGHTGKVDGVAV
                     HKGMTITQDTADRLLRDDLSWVEHCIAERVTVPLNQSQYDALCSLIFNIGADAFIGST
                     VRRQLNAGNYTAAADAFLKWSRAGSNPTILAPRRGRERAMFLGQR"
     gene            24670..25134
                     /locus_tag="OVW45_000695"
     CDS             24670..25134
                     /locus_tag="OVW45_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCV5525218.1"
                     /translation="MKRATLAFIVFMVVAVAVLGFTADYWHNRFTRAEKARQKAVNLA
                     DTRQQTIDDMTLRQRQNAALDAKYTKELANAKAESEKLRADLASGRRRLQLHAVCMPA
                     ATRDTTATGSTDATAARLTPDAERDYQHLRAEAKAVVAQVNGLQQYITEQCH"
     gene            25165..25437
                     /locus_tag="OVW45_000696"
     CDS             25165..25437
                     /locus_tag="OVW45_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail assembly chaperone"
                     /protein_id="HCV5525219.1"
                     /translation="MKDENTTIITLTVAGEDIRFAPTEAVYNKFVNEITMDNKVAPAK
                     NYLMRCVHPEDKEKLEKLIHRPGAALQMAAKLNEDFAVNLDITVKK"
     gene            25625..27706
                     /locus_tag="OVW45_000697"
     CDS             25625..27706
                     /locus_tag="OVW45_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCV5525220.1"
                     /translation="MAAELDFTLSLVDKLTRPLKQAQSAVTGFADKATADFKRLGFGV
                     AGLWGAAQGIKGLVNPARDMEAALAEVSSLDVANKTLDQLRKTSQTFAINYGESASNF
                     VRSAYDIQSAIAGLQGDELPKFTEASAILAKATKSDTATITNYMGTMYGVFKNTAEKM
                     GRTQWVEQIAGQTATAVQVFKTTGNEMSAAFTALGANAQAMKVSAAEQFAVLGQLQST
                     MSGSEAGTKYKSFLAGIGNAQKVLGLNFTNRDGSARSITGIIDLIKGKFGDLSKVADA
                     DLLKKAFGSDEAVSMIKLLAGDVDGLKRNINTLGNIKGMDKAIEMAKKMVDPWDQVNS
                     LLEQVRVSIGLRLDPVFAPFLQKIITGGKAFMKWLDTFPNIARWLGYITAMTLAFAAA
                     GALASITMGAFGFIMTGLSGIAGVLKGAWKGLIFTLDLLRPSLLSTRLGLMGLWIQEK
                     AVWVWSKLVALWAGFCKAAIVAWNVVLRAGAVAMRLWGVATAFAGGALQLLISPITLI
                     ILAIAALAVGICLAIKYWDSIKAAIMDTEAFSALLKVINAVVGWFDDAWNSIKAGWDN
                     LTNWFKNFSLADTFGNITAGIGKLFDGIWDSIKSAFTGTWNWIAEKLNKIPGVNISTA
                     QQTVAPTPAPELITGNRSAAIQGGPVSHQISNNRGDKVTNNNVKQDITLHVDTLATPG
                     QLAEYSELAAG"
     gene            27710..28048
                     /locus_tag="OVW45_000698"
     CDS             27710..28048
                     /locus_tag="OVW45_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2590 family protein"
                     /protein_id="HCV5525221.1"
                     /translation="MSTKSLYIDLLITDGDLTFNSASEPVLCDNRQSIAQDMIHALIE
                     SGLPCRLIAENSPTLRADLFTQMVILLEEDERLIPGSVFIDEEQRGQLLVTADTYDFG
                     QLSQGMRYAE"
     gene            28038..29216
                     /locus_tag="OVW45_000699"
     CDS             28038..29216
                     /locus_tag="OVW45_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008812617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCV5525222.1"
                     /translation="MQNNIDFKNILRESGMPVDEQTVRDTLQQVADDEKLITNTSRMS
                     PFWRLIQLLVITPYVWIVDTLADNVLHNLFLMTARGPFVDLFAAALKLTRKEATRAIG
                     KIIFTKNNQNDSVTVPAGTLIQTERINGVIYSMATREQVIIPAGTQSTLIDVIATDSG
                     TAFNLAPGYYQILPKAINGIASVRNGDNWLTIPGANRESDDELKDRCRNQFNLAGSYH
                     TDAVYRSLIAAQAGLTIDRIFFLHDAPRGPGTTNAYLLLDTGVISQPWVDSVNDYIMA
                     QGHHGHGDDMRCFAMPETRHDLAVTVYVENLSNISKNDIDALKSGTENLIRCAFRENS
                     NYDVTRTWPYSRFSFSQLGRELHDTFPLVGSVTFSLEDIISDLNVPRLNTLTVEVKNA
                     "
     gene            29209..29781
                     /locus_tag="OVW45_000700"
     CDS             29209..29781
                     /locus_tag="OVW45_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013575830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525223.1"
                     /translation="MPDKSPDIKLPSWLNRGDVLRLKNTFIRFWGKVHDWVTWPLSQT
                     DPLTCAESILNLTAWQYDIARFDGEPLTLYRKRVKYAFINAQDAGSVAGFRAIFERLG
                     IGYVEIQERQPGTDWDVVLLRLTDNQISENTTLLNQIIRQYGRTCRRYHLQIITSTDC
                     VIGHGYWHGSYHYFYAPEMKNDPERYHFSI"
     gene            29750..31756
                     /locus_tag="OVW45_000701"
     CDS             29750..31756
                     /locus_tag="OVW45_000701"
                     /inference="COORDINATES: protein motif:HMM:NF023985.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCV5525224.1"
                     /translation="MTQSAITSAFERLKAQEAAGGNRIIIDQFVFANIPDLNIADTPS
                     ENEPLPPDAQIVHRQNVDRNGMVNENTVAYSVTLPESTGDFTFNWLGLVSSTTNTLCM
                     VVYLHPQKKIKTADGKQGNTLIYSEIMEYAGASAATGITTPVSTWQIDFTARLHGMDE
                     ATRKAALDIYGPGLFFNNAFKLTAQATGQATFAPGIAYLRGLRVELDEQGTLTYATGI
                     SQTVYVDAALTGTLTGENKAIFSLTSQKTADYTDSSGFAHYVEPIATISDSGEITDIR
                     KTRKPLNDRLDGEYLTRNGNLKEIADKGEQAQSDARKHMGLGDSATLNVGTAQDTVAA
                     GDDIRITGALQKNQNGADIPDKPLFIQNVGLQETVNKADNAVQKTGDTLSGGLTFEND
                     SILAWIRNTDWAKIGFKNDSDADTDSYMWFETADNGNEYFKWRHRLAGGRVKDLMNLK
                     WDSLNILVNAVINGCLGIGTTNALGGSSIVLGDNDTGFKQNGDGLLDVYANGQRVFRF
                     QNGVTIAFKNIQAGTARKFTLSSANNSTKNAAFYLWGNPSRPVVAELGDDSGWHFFSQ
                     RNTDNSITFAVNGQVIPLNYGNFDARYKHRTEGVQDVRYGHEMYYNPGSNTVSWTFRS
                     PSGHGLSGISISDTGRNSADNVNGVYYRPLQKLINGTWYNVASI"
     gene            31758..32285
                     /locus_tag="OVW45_000702"
     CDS             31758..32285
                     /locus_tag="OVW45_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCV5525225.1"
                     /translation="MMHLKNIKAGNAKTPEQYELTKKHRVIWLYAEDGKNWYEEVKNF
                     QPDTIKIVYDENNIIVAITKDTSTLNPEGFSVVEVPDITANRRADDSGKWMFKDGAVV
                     KRIYTAGEQQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTAKPEWPQKPG"
     gene            32422..33165
                     /locus_tag="OVW45_000703"
     CDS             32422..33165
                     /locus_tag="OVW45_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525226.1"
                     /translation="MWFPAKLMLPTGNITPITCSAMAVHPWDVEAGHITPDGAYLSPV
                     NAINHLNSKLPGLTENSDIAIIMVTGSNNNDFIRQLSALADTLPLPTLTQTLRRARTQ
                     LTQAITRMQIPATPQNGLPAPQPLIASTLQNSISNHTALNAMKAEGLNSVDSLKNALS
                     GFQAQRQRLQKQIIAELAGAGDKVAKAFAFVHTGDAVLARREMMKNIPHPTASLTYAH
                     LFAGDLSGMLNWITKAENEPEHDNATAGP"
     gene            33128..33694
                     /locus_tag="OVW45_000704"
     CDS             33128..33694
                     /locus_tag="OVW45_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCV5525227.1"
                     /translation="MSQNTTTPLLALNGQIIPLKRLSVSVKLTIKDKDASGKSSSTAT
                     SEQGVKAKELQISGLIPFTQPEALTRLFRLAEARAANGAQQVYRIANMDAKAVNMKQG
                     IFSGAVGATPETGLMAWKIDFTLKEKLSSAEKATGRGPAGSQSAEQHAQAVKSGGKGE
                     KNEEHGWFWKMSDKLNNWIGPAGNETNS"
     gene            33726..35366
                     /locus_tag="OVW45_000705"
     CDS             33726..35366
                     /locus_tag="OVW45_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525228.1"
                     /translation="MEENIMLRLNGAGTGFITINAADNTPPLRGKPVELSIGYNDQPV
                     KWFSGYVESDSCTGRGLHKLMVREAAAILQYPLNISMQHPTLKQVAGHIEGNTGLRIQ
                     LPKAGYTTTPVPHLTHNGNGYQLMAMLGRIFSIPDYVWYPSVDGIIYAGSFADCRFAK
                     RPARLPVDITRGDHGANGWTIQTIPMMRPGVVMNDHRINQVQLQGDSMIISWSDGRQS
                     PVQRQIETLYPEIGNKTHLPRMGRVISPTENTTQGDLHDEFRPRYAVNVQLLDENGTP
                     DKNTPVYNAVPVPVPMAGNESGIFQYPPPGTIVTLAHVDGRPDKPIITGTHASGQSLP
                     GIKPGEQLQQQRAEVFQRVHTDGTWQRETDQVIREKSTDRIIQNTTETRTSTTRNITV
                     KANNTITVLGTHKLMSGHIQHIADGDYAVAATKKLIQHADSAELDVTHQWQTSTESTT
                     HNVAKTLEEKIGRIKKSVAGQMQQIIAPQVWFGSNTINTLNLMLDLCDTVQQLAQLTA
                     QHTHTNNGSSQPTNSGSIDTVASMAGNLKAKYSTVIKQ"
     gene            36308..36451
                     /locus_tag="OVW45_000706"
     CDS             36308..36451
                     /locus_tag="OVW45_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HCV5525229.1"
                     /translation="MSQKSLIAITTLCVTAILIIWMLQGSLCEIRMSFWGAEFAAFLQ
                     CRQ"
     gene            complement(36760..37059)
                     /gene="ihfA"
                     /locus_tag="OVW45_000707"
     CDS             complement(36760..37059)
                     /gene="ihfA"
                     /locus_tag="OVW45_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCV5525230.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            complement(37064..39451)
                     /gene="pheT"
                     /locus_tag="OVW45_000708"
     CDS             complement(37064..39451)
                     /gene="pheT"
                     /locus_tag="OVW45_000708"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCV5525231.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            complement(39466..40449)
                     /gene="pheS"
                     /locus_tag="OVW45_000709"
     CDS             complement(39466..40449)
                     /gene="pheS"
                     /locus_tag="OVW45_000709"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCV5525232.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLLGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     misc_feature    complement(40658..40782)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            complement(40732..40776)
                     /gene="pheM"
                     /locus_tag="OVW45_000710"
     CDS             complement(40732..40776)
                     /gene="pheM"
                     /locus_tag="OVW45_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCV5525233.1"
                     /translation="MNAAIFRFFFYFST"
     gene            complement(40899..41255)
                     /gene="rplT"
                     /locus_tag="OVW45_000711"
     CDS             complement(40899..41255)
                     /gene="rplT"
                     /locus_tag="OVW45_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCV5525234.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     gene            complement(41308..41505)
                     /gene="rpmI"
                     /locus_tag="OVW45_000712"
     CDS             complement(41308..41505)
                     /gene="rpmI"
                     /locus_tag="OVW45_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCV5525235.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            complement(41602..42144)
                     /gene="infC"
                     /locus_tag="OVW45_000713"
     CDS             complement(41602..42144)
                     /gene="infC"
                     /locus_tag="OVW45_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCV5525236.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            complement(42148..44076)
                     /gene="thrS"
                     /locus_tag="OVW45_000714"
     CDS             complement(42148..44076)
                     /gene="thrS"
                     /locus_tag="OVW45_000714"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCV5525237.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            44601..46499
                     /gene="espL1"
                     /locus_tag="OVW45_000715"
                     /pseudo
     CDS             44601..46499
                     /gene="espL1"
                     /locus_tag="OVW45_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            46671..46778
                     /locus_tag="OVW45_000716"
     CDS             46671..46778
                     /locus_tag="OVW45_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525238.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(46831..47589)
                     /locus_tag="OVW45_000717"
     CDS             complement(46831..47589)
                     /locus_tag="OVW45_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCV5525239.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            47876..48805
                     /gene="pfkB"
                     /locus_tag="OVW45_000718"
     CDS             47876..48805
                     /gene="pfkB"
                     /locus_tag="OVW45_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCV5525240.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            48906..49196
                     /gene="ghoS"
                     /locus_tag="OVW45_000719"
     CDS             48906..49196
                     /gene="ghoS"
                     /locus_tag="OVW45_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCV5525241.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            49302..50162
                     /locus_tag="OVW45_000720"
     CDS             49302..50162
                     /locus_tag="OVW45_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCV5525242.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQLQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(50203..50739)
                     /locus_tag="OVW45_000721"
     CDS             complement(50203..50739)
                     /locus_tag="OVW45_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCV5525243.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            50886..51554
                     /gene="hxpB"
                     /locus_tag="OVW45_000722"
     CDS             50886..51554
                     /gene="hxpB"
                     /locus_tag="OVW45_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCV5525244.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            51717..52307
                     /locus_tag="OVW45_000723"
     CDS             51717..52307
                     /locus_tag="OVW45_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCV5525245.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            52440..53831
                     /gene="tcyP"
                     /locus_tag="OVW45_000724"
     CDS             52440..53831
                     /gene="tcyP"
                     /locus_tag="OVW45_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HCV5525246.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(53835..54651)
                     /locus_tag="OVW45_000725"
                     /pseudo
     CDS             complement(53835..54651)
                     /locus_tag="OVW45_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843061.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(54928..55191)
                     /gene="cedA"
                     /locus_tag="OVW45_000726"
     CDS             complement(54928..55191)
                     /gene="cedA"
                     /locus_tag="OVW45_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCV5525247.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            55374..57635
                     /gene="katE"
                     /locus_tag="OVW45_000727"
     CDS             55374..57635
                     /gene="katE"
                     /locus_tag="OVW45_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCV5525248.1"
                     /translation="MSQHNEKNLHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVLIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(57893..58651)
                     /gene="chbG"
                     /locus_tag="OVW45_000728"
     CDS             complement(57893..58651)
                     /gene="chbG"
                     /locus_tag="OVW45_000728"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCV5525249.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMYFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNSG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(58664..60016)
                     /gene="celF"
                     /locus_tag="OVW45_000729"
                     /gene_synonym="chbF"
     CDS             complement(58664..60016)
                     /gene="celF"
                     /locus_tag="OVW45_000729"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCV5525250.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPACELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(60121..>60757)
                     /gene="chbR"
                     /locus_tag="OVW45_000730"
     CDS             complement(60121..>60757)
                     /gene="chbR"
                     /locus_tag="OVW45_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HCV5525251.1"
                     /translation="DFVFIPLGSHHQSFYEFGATRILNVGISKRFFEQHYLPLLPYCF
                     VASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRHYREEQVIDDVP
                     QWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTPMQIINEIRINF
                     AKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLTEFNQ"
BASE COUNT        15994 a        16193 c        14538 g        14032 t
ORIGIN      
        1 ttcgtctcgc ctgccagaaa tttttctgcc aatagacatt atcaagtgag gaacgcatca
       61 ctcccttgct ggtagaggcg tggataaatt ggttgttggt atcgtaaata cctacatgca
      121 aaccattttg tccggaaccc gttttgaaaa agaccaggtc accaggcagc aactcgtctt
      181 tatcaatttg cgtgccgata gaggcttgtt ctttggtttc tcggggcagc tgcaaatcga
      241 aacgatcgcg catcgttaca accacaaatc ccgaacagtc cacaccgcgc cgcgtcatgc
      301 caccataacg atacggcgtg ccatgccagc tttgtagctg gtcgttcaaa ccggcaataa
      361 cggtaatcga atcagaaagt ctggcatttg gcggcggtgc tttatggtgg ctacacccgg
      421 ccagaaacag tgctgtgatc aaaataaggc agaaacgcat tccgtacggt tcctctgttt
      481 tttattcttg cattaattta gcgtcgtaat tacccgattt tcaagatact aatgaaatca
      541 gatggtcgaa atcagcattc tgtgaccttc gatatccaga cggcgaaaat tcatcccata
      601 ggcctgcgcc agatttggcg gcgtgagcac ctcttctctg cgtccactgg ccagcatttt
      661 tccacctttt agcaaccacg cccgatgcgc atgacgcaat gtgtggttga gatcgtgact
      721 gctcatcaca atcgccagtc cttgctgaca cagagcgctc agaattttgt ctaacgcact
      781 ttgttgcgca acatcaagac tgttcatcgg ctcatcaaga agcagcaatt ggcctgcggg
      841 attggcttgt ggtgtgattt gcagcaccac cgcagcaaga cgtacgcgtt gccattcacc
      901 gccggaaagt tgattggtgc tacgtccgag tttgtcatca agagccagcg cccctgcgac
      961 atcattcagt agttcggtac gcgttttatc gtgctgatgc agtgtcagat agtgccagac
     1021 cggcattgca aacggcggcg tctgctgttg tgaaagatag gcgcgatgca gcgcgagttt
     1081 tgttgcggac catgcttcca gtggttgccc cgcgaactga atacttccct taccgctggt
     1141 cattccggcc attcgtgcca gtaaggtact cttacccgcg ccattcggcc ccaccaggtg
     1201 caggatctcc ccagcccgaa cctcgccaga aagtggcccc aggcgggtag attccgcaac
     1261 atcctgtaac tgcatcacaa tagacattat tttgccaacg ccagtttaat gctttccatc
     1321 acaatggggt cttccggcgt catatccggg gaaaaacgct ggatgaccaa tccgtccctg
     1381 ccaactaaaa atttctcaaa attccataaa atatcatcag ggtacagcgg tgcacgacct
     1441 ttgctgacca tacgggcata gaatccgctc tcttccggcg cgactgcggt cggcgctgcg
     1501 gcaatcaatt tttgatacag cggatggcgt ccttcgccgt taacttcaat cttactgaac
     1561 atcgggaacg tcacccccca tgtggtggta cagtaagttt taatctcttc atcgctgccc
     1621 ggttcttgtt ccagaaactg gttgcacggg aatcccagca ccacaaaacc tcgatcggcc
     1681 caggctttct gaatattctc caactgctca tattgcggcg ttaagccaca ctttgaggcg
     1741 acattgacaa tcaacagcac attaccggcg tacttctcca gcgtggtcac ttcaccgtcg
     1801 atatctttca ctacggtcgt cagaatggaa tcttgcatcg tttctcctgg gtgtggtcag
     1861 taaaaatctt agcttttaat catagaccgt ctttttgcgg ctaacgtcct gcttttaaca
     1921 ataaccagat aaacaccggc gcacccaacg ttgcggtgac cacgccaata ggcagctctg
     1981 cggcagctaa tgccaggcgc gctacaatat cggccagcag cactgcgctc gcccctgcca
     2041 gcgcgcagcc gggaagtaat acgcgatgat cggttaaacc acacaaccga agaatatgtg
     2101 ggatcaccag accaataaag ccgatagcac ccgccagcgc cacactgacg ccaaccatcc
     2161 agccggtcgc tgccaccagc acattgcgcc agaaccacag gggtaaaccc agttgccgcg
     2221 ccgagatctc gccaagtgct aacatattca tcggcctgga ctgacaacag atccacaaca
     2281 acacggggat caatgccagc atcagccagc tttgccgcca gtctacgccg ccaaaaccgc
     2341 ccatcatcca gtacatcagc tgacgtaaat caacagaggt ggaaaagtag atagcccacg
     2401 tcattagtgc gctacagata atccctaatg caacgccagc cagcaataac cgactggtcg
     2461 aaagatgacg acgggcgaaa cgtaagagta ttaaagtgat gataagcgcg ccagcaatcg
     2521 cacacagccc tagcgcccag ttggggagtt gcccttgccc aagcaatacc gcggcgataa
     2581 gccccacgcc tgcgccgtta gagacgccaa gtagtccagg ttctgccaga ggattttcaa
     2641 acaacgcctg cattacagcg ccggatatag ccagcgccgc accaaccagc aatacagcca
     2701 gcgtacgtgg caggcgaatt tgccagacga acagttcgcc acgaggagta aaccagtcac
     2761 ctggcgaaat ccattgttca ccggcgcaaa ggcttaagag aagcgccagc agcatcaaaa
     2821 ctgacaggca taataaccag cgaatatttt gtcgctgttg ttggcgggca agtgtcagca
     2881 tggtatccgt tctgctgaag tgtcatggcg ttgattttac ggtgactctt cgacagtgaa
     2941 aagaaaaaag gccgcagagc ggccataatc acaacccaaa accaataagt tacataatta
     3001 tcaaagagtt acagacacac aaaaacacag ccaaccacaa caaataacat agatgtggtc
     3061 aatttgtgga tcatcatcca acaaatttag caacaaatac agcaaccgcc gccgtgaaaa
     3121 tggttgtgca tactggcaca ataatgccac tccaacgcca cttcgtatta tcatcaacct
     3181 tgtcaaaccg ccgattcatc tcatccataa tcttgtcaaa gcgtttatcc atcgcttcgg
     3241 taagctcacc cttcaggctg ttaatgtctg atttgagctg ctctttcagg tgagctgtat
     3301 cagatttaag ctcacctttc agattcgcaa tatcgcattc aagagcacct ttcagatgtg
     3361 ctatgtctgc tttgagttca cctttcacct caccaacatc ggctttagtt gagaggtttg
     3421 tagtgcgttc agcaagcgtc gcaaggtcgt ttcttgttag cgataaatca ccttcgagcc
     3481 gctcaattcg cctttcaagt ttatctgaca ttccgcctcc actactgccg ccatcgccac
     3541 catgataaag cacatcacgc tcagaatgcg acacactacc tgctgaatta ccgaaaagat
     3601 ttcttgctac attacccatt atcaccatcc tgcgctttgt ctttctccaa ccacttcagc
     3661 accgcagacg tacgataaag gctcaggtaa ccacaattca ggcaatgcag cgtgtaataa
     3721 gttttgcgca tatcaggtat gctcttaaca tcacccaaaa aagtataaga tacataatgc
     3781 tttatgttct ctttaattaa ctcactctgc tcctccaggg gtaggttgtt aaaattttct
     3841 ggtttcttat ttttatccca gcaaagatcc atactttcag gaacagataa tcgcgacgat
     3901 ccacatgaag ggcagacact ggagaccccc ttctcagaca agaaacggaa gaagatttct
     3961 ggcgtaatct ccatcagccg ctctttatgt tttttgacac gactcatcga cttcttattg
     4021 atttcatcag cttcttcttt gcatttatct tctatatatt tcgatagtaa atagtcatat
     4081 aactcttttt ttgtaggttt ttttatttcc atagttacat tcctcccaag ggattaaacc
     4141 tcaccgcatc ctgcaagtaa tccggcgcaa gatgggcata aatcatcgtt gtctgaatct
     4201 ttgcgtgccc cagaattttc tggagcgtca gaatattgcc gccgttcatc atgaaatgac
     4261 tggcgaaggt gtggcgcagc gcatgaacag cctggccgtc aggaatatca ggtgcgaccg
     4321 ttttgataac atcacgaacc agtggataat ccagtgttgg aaacaccagc tttccgcccc
     4381 gttttttgat cttctcaaac aggctttcag agataggaac ggtacggttt ttactgttct
     4441 ttgtttttga aaacgtgatt cggcaatgaa gaacgcgacg ctgctccagt gcctctacct
     4501 cgccccatcg cgccccggtc gacagaagga tttcgacagc cagccgttca tcgggatttt
     4561 cagccagtgc atccagcaac tgaacacatt cagacttact cagatatccc atttcgcgct
     4621 cgttaacctt cattccttta aggccttgaa cggggttatc gttaagaaaa tggccggatg
     4681 agatgagtgc ggtaaacatc gcgcttaacg ccccaatctc tcgatttatg gtgctgggtt
     4741 gtatcccctg ctctatcctg tacacacgca gctcggtgag catcgttgta ttaagtttat
     4801 gcacgcacgg gtcatccatt gcctcactca agcgcagcaa tttaaggtgc gtgttatgcc
     4861 ctgacctcat taactggccg tggtatttcc accacaagtc aataagcact gacagcggac
     4921 ggcgatcaat ggagtttcct ttccactcat tgttatgctg ttgcgccagc acccaccgct
     4981 catataaaac tgcatccgat ttcgttttaa attttttgcg aatgcgtttg cctttacgcc
     5041 cctcagggcg catgtcaaga agataccctc ccggaattga ttttatgctc attcgtgaaa
     5101 ccccggcgtt acaagaccac catgccccca gcgttccatg attagccggg ctgtgtgcca
     5161 gtcttgcggg ggctttgtga acgagatgtg acatcgtacc catcagggga gagagccggg
     5221 ctgatctgcc ccgcagcctc atttgttttt cctgtcataa gccacattgt gtacttttcg
     5281 aaatctttag aattaattac gcgatcgaca acgcttaaac caacctctct tttgccactc
     5341 tcataattct ttatggttcc gagatttatc ccggtaacat cggcaaactc aacttgagtt
     5401 aacccttcac tcttccgtat ctctttcagt tttttttcat acccacttga catggcagtc
     5461 tccagacgac taaattaata tcaaaagtag tctaccgacg actttttggt aacaaataac
     5521 cacagataga acaggttatc acatcatggc agaggtcttg aatacatacg aacaggaaga
     5581 cattgagcgt ttagcggcgt tctacccata ccgcgatgag catggattac caatacttga
     5641 agaaagcctg aaagattacg caaagcgtac caaccaagct gttaacacag tgaaaagaca
     5701 ggctgacaga ggttcgattc ccctcacacc tggtaaaaag aactcaagac gcacagtaaa
     5761 tctcttcgca attttcctga aaacaatcag gagcgcagag aaatacgtgc agatgacaaa
     5821 ataacgaggt gtcattttat gctgaagcaa cgccgtaatt ttcgcaccga aacggaacgc
     5881 caagctaacc gtttcgctac cagcgcatca cgcagcaaca tccgctacag cctgagcgag
     5941 acgcacgcaa cgccagatgg ccatacagta aaacaaattg gcgagcatac ctggatgatt
     6001 gagaaagctg gaatagtggt ccacaaatac ccgcgcaatc cgtttaccgg aaaccgcatt
     6061 tttgctctga gctgcggcga caatcagttc gggcaggatt tcacgttgta cgaagcgcta
     6121 cgcacagttg atcgccttct ttacggagaa aattttatta aacaggctga gttataacag
     6181 cgggtttatc accggttatt tttaacgagg actccgttat gagaaaacac acagcagaac
     6241 aggtaaacga atttctgcag ggatattact tcgataacga aattaatccc cgcgccagaa
     6301 aaacacactt cgaagtaatg aagtgcggaa tattcagcgt ccgcaacacc ctgttttgtt
     6361 caaaagatac gggagccagc gaggacctta aagaacttaa ccggatggcg aaacaattaa
     6421 cggatggcat ggttcccgaa cccgcaataa ttacggagta atcacgatga atttcagtta
     6481 ttcattcagt gtaccggagt cgttatttgt tatcacactg attgcagccg caatgttttt
     6541 tgccatctgc atactgacac ttattgatac atggcgggaa atacgcgaca gacgtcgcca
     6601 gaaaaaaaaa cgggatgcat acaacgcatt cattcagcac aaagaagacg tagagcgtaa
     6661 agcacgccgc ctctttagtt aattaaccgg tttcgttaat acaccgctca cgcggcggga
     6721 ttcgcacaat ctgaataagg gaaataaaaa tgattaatcc gacactcacc ctcaaagata
     6781 aagcccttgc gggcgcaatg tttttacgca actacgccgc aatgatggct gacactgaca
     6841 acccgatgat ggtgctgtca gtggagcctc accttatcga cgcagacgca attgaacagg
     6901 tggtacagga aaacacctgt ctgagaaatc agcttgccac ccttcagaca gcagctgacc
     6961 cggcaaagga aagtgctgaa cacacctgcc acaccacatt catcaaaggg acccgcgtat
     7021 gtctgaaaaa cagtccgcag caacgcggaa cggtcgttga cacccgcacc agtagccccg
     7081 ccattgaccg ggttttcgtc cgtttcgatt cccccttcga ggaagaccgc tgggtaaaga
     7141 ccagaaacct ggaaataacc cccgacaaat gaagctgatt atccctgcgc gacaccgcaa
     7201 aaaccggatg aagccagggg gaaagccgga gcgggcagca agggcggcat accgtcgcct
     7261 gctgaagggg gaccggtcac acgtgcgacg gctggagcgg gtaccgcccg cgtcatcaac
     7321 gtcatgtacc gttaccggct gttaagtctg gacgaaggcc ggacatgggt actgcttaac
     7381 cataacgaat acgtgaagag gataagacga tgttacagat gaaatttaag ccctgcttta
     7441 ttgacgcatt ccgcaacgga cgcaagacca catcccttcg cccgacgggg tttgattgct
     7501 accaggctgg ccaggagtca gcagaatatt ttcatgaaga caccctgacc cgcaacatcg
     7561 ttcttcccga ttacagcgcc ggcggtaccc tggtttttga ggagggaacc acatttaccc
     7621 gcgcatcaga ctttgccgga ctcctgaaaa gacagcccta ccggcagtat gacaacattc
     7681 atctggtcac agaaaacaag aacggcgaaa ccgtgccgtt cgccgtggcc ttcattgccg
     7741 atatcggtgt aatacagggc cgccagataa ccgatgaagc ggccgtcagg gacggcttta
     7801 accctgaaaa ccatccccgc gacgaactct ttgcattcat gcgggacacc tgccacatag
     7861 aaaaccccaa aaacgaaacc tactggcggt acaccttcac ccacatcgtg atgttgtcgc
     7921 agtggagggc tgacgcatga aacccgccat tatctgcatt ctgtgcgaga gcactgtcac
     7981 cggcgaccat taccgcatca gggaacgcca catcaaccct gaccgttcgc tgctggacaa
     8041 catcaccggc ccggatgatg aacgcgtgat ctgcctgaac gacggctcgc agttacgcgt
     8101 gcgcaatatc cggcgcgaaa tcacgctcga acccacccca ttcacacgcg ggaaaaaacc
     8161 ggagagtgac gcatgaaagg tattctgaac cagcgctcac cgctcaaatg ggcgggcggc
     8221 aaattcagta tcatgccgca gctgcgcgaa tatttaccca aagccgcctg cctgatcgaa
     8281 cccttcgtgg gcggtggctc tgtcttcatg aatacggatt acgaccatta cgtattgtgt
     8341 gacagcaacc ccgcgctgat taatttctac cagcaactga cgaccaacac catggaatta
     8401 atggatcgtg catggtcact ctttaaggat ggtggcacac cggaagccta caaacgccat
     8461 cgcggggcat ttaacaccat cgcgctggcc accgacagat tacgcggcga atccctggaa
     8521 tgggcagcgc tgtttctgta cctgaaccgc tattgcttta acggcctgca ccgcaccaac
     8581 caaaaaggcg aattcaatgt gccattcggc aaacaccgca caccttattt ccccgatcag
     8641 gaaatgcgcc tgtttgctga taaagcccgc gagacacgaa cccgctttat tcacgcggat
     8701 ttccgtacag ccttcacaat gccggctgag atccgccgtt tttcttcagt gcacggcggt
     8761 atcgccattt actgcgatcc gccctacctg ccgccggacg gtaaagaggc attcactcac
     8821 tacaacggcc gggcattcac ccgtaaagac caccgtgacc ttgcggcgct catgatgaac
     8881 gcctggcagc gttacggtat ccgcccggtg gtctccaaca gtgacaccac cgaaacccgg
     8941 caaatctatt cctcgtttga gctgcacacc ctgagcgttc gccgctctgt tgctgccagc
     9001 agcagcgcac gccagcaggc aaaagaggta attggtgttt atccgccagc ccataaaagc
     9061 cagctccggg acgtcaatgt gggctggccg tctccccttc acgctccaca ccccaccacg
     9121 gagatcatgc aatgaccgtt atctatatcg ccggccccat gacgggaaag cctgatttca
     9181 accgcaccgc cttcaccatg acagcgaccc gcctgaagat gcagggccat accgtactca
     9241 accccgcaac actccctgac gggctgagat acagggacta catgcttatc gggagcgcca
     9301 tgctgcactg tgccgatgtg atttacctgc tggatggctg ggaggactcg ccgggggcaa
     9361 aagaggaaca cgccaccgcc ctgaaactga atctgatcat ctccacacca gaaagccgta
     9421 aggaggccaa atcatgtttc tgaaaccaat ggggggacca ggtaaagcac cgaagcacgt
     9481 cagtgcatgg acacaacagg aagacgaact gcttatctcg ctttatcagg atcacagcac
     9541 atggcaaatg gctgaacgac ttcagcgaac ccgcagcgca gtgatgcacc ggattctttt
     9601 tttacgtaat cgcgggttga ttggtcgcaa gaaaaaagca ccgctgagtg cagaagccat
     9661 cgcctttctc atcaaaaacc gtcacgcaaa aaccgcacgg gaactggccc ggaaggcggg
     9721 atgcagcgag tgcaccgtga ggtacacact gcataaacgg ggttacagcc ttaagaaatg
     9781 cggcgagagc caccattgca ccagatacag cgatcgtctg gctgagctgg ttacggagct
     9841 acgcgacaga cgcaacatga cgttctgcat aatcgcgaaa cacatcaaca tcacgatgca
     9901 aatgcacatc agcgatgata cggcgtttca cctctacaac cgccggaccg ccgccgacgc
     9961 cctgttgtac gaactgctgc cgaactgagt gatcatcatg cacacacaaa aaaaccgctt
    10021 gccatgtcgc aatcagtcag gttacatttc cgctgcacct cataaaacgg gtgccgggtt
    10081 tcgcagcccg ctgacaaaca tagcgcacaa ccgcgccagc ggtttttttg tgcgtactgt
    10141 atcgccacat ctttttcgca cacgaattat ggtggggcgt acggggccga cttcggtcgg
    10201 gccgggttct atgtttgccg gtactgcgaa cctcgtacgt ctcgccaccc acagtttcgc
    10261 agctctggat ggtgagtttt caaaacttac acacatagag gccacatcat ggcaaaccgc
    10321 aaacaacagc gcgcatacgc tgcgcgtcgt cacatccgga ctgaaatcaa ccgcagactt
    10381 ttccgcgcat cacgcgtcgc gcaaatcatg cacatcaata tgctgcatga acgcagccac
    10441 gcgctgtcag acagctactg cgccgctgta ttcagctatc tggcggatga tctgcacgag
    10501 cttcaacagc tcatccagca gcaaaacaaa ctccattaat tcctgttccg ggcctttcct
    10561 gcaccttgcg gcgggaggcc ttcgcacatc tttaacaaag cgaggtgcag catgattgac
    10621 cgtcatgact tcgtaaaatg ggtgcgcaca caggacaccc gtctggctcc aaaacttcag
    10681 gcaatgtttg atttctatct ccgtgcccgc gccagccagg ctcgcaccac aaaaccagag
    10741 aacgcaggcc ccctttattt catcgtggac gactgttacc gcgtggactt cacaccacac
    10801 ggtctggccc tgtacaccct gacaccacac ggcgactccc tgctggcgta ttacaactcc
    10861 cctgcctccg tgtttgcagc aatgctggcg catcgcaccg ctggcgggtg tgcctcgctg
    10921 agtgaataca ccactgagtt taaccgtctt tctgccctct tctcgcagga atggcaacgc
    10981 gtgacagggt gtcaaccatg agtgaattta cctgggactg gaacgaacca cggcaggcca
    11041 ttgacccagc cacatttgcg aagtatcagc ctacgcctga aaccccgttg caacaggcca
    11101 tacgctacta ccaggaagca gatctgaaag cacaggaagt cagggaggcg gaagaagaag
    11161 ccttttttgt gcagtcagac atgggtaaaa aactcatggt cgccctcgat gaggacgaac
    11221 ggcgcgagaa ggtaacacaa agcatcatca gcaagcgcca ggcaacagaa caagacccgg
    11281 tggcccgtgc atttgtcaca ctaaaggcgc tacctgttta tctgcgtgaa cctctgagcc
    11341 gccgcctctc ttttttacgt aagaagcagg aatctgaccg ccagaaaggc aaaaagagtc
    11401 gaccatcaga gggctacgcg cgcggaaccc tgcgcaaaat attcgaacga ctggaccgca
    11461 ccgacagccg ctggctgaca ccgggttatc gcgcccttgc cggacgcgaa cgcctggacg
    11521 atttgctgta cctgccgcag cttaacaagc accagataca gacgctgggc accatgacgg
    11581 cggcaatgtt cagcagctcc tttgaaacac tctgtgatgg tttcggtgcc agagatggcg
    11641 agctgaccat ggaggtggca ctgaaggcgt atcagatgct ggcccgcatg gcgctacacc
    11701 tgcacgccat gcctccacat tatgacgcgc tgacaacaga caaagaccgc aagaacgaac
    11761 cggacacgga actgctgccg ggcgcaatcc ttcgcctgac ctgtgcggac tggtggaaac
    11821 gcaaactgtg gcttttacgt tgcgagtgga gagaagaaca actccgcgcc gcctgtctgg
    11881 tttccagaaa aacatcgccc tatctgagcc aggacgcgtt aagtgagttt cgcgcacagc
    11941 gcgagaaaac acgcgatttc ctgaaaagtt tcatgctgga aaatgaagac gggttcacga
    12001 ttgatctcga gacagtgtat tacgcgggag taagtaaccc ggttcaccgt aaggcagaaa
    12061 tgatggccac catgaaaggg gcggaactta tagccgaatc ccgtggcgac aaagcggtgt
    12121 ttctgactgt cacctgcccg tcaaaatacc acgcaacaac ggagaacggt catccgaacc
    12181 ccaaatggaa cggggctacc atgcgcgatt ccagtgatta tctggttaac acttttttta
    12241 aagccgttcg taacagactg gaccgcaagg gcctgcgctg gtatggcatc cgcacggtgg
    12301 agcctcacca tgacggcacg gtgcactggc atatgatggt ctttgcacat cctgaagaaa
    12361 tcgacagcat cgtgaccatc gcccgcgata ttgccattcg tgaagaccgc cacgagctgg
    12421 gcgatgacat aactccgcgc tttaaggcgg agtacgtcga cggttcgaaa ggtacgccga
    12481 ccagctatat cgccacctac atcggaaaga acctggacag ccacgccgtg gatggcattg
    12541 acccgaaaac aggcaaaccg cgcatcgacg acgaatcagg aaagacgatg gcagaaagcg
    12601 tggaacgcgc catcggctgg gcgcgccttc accgggtccg ccagttccag ttctttggca
    12661 tcccctcccg tcaggtatgg cgtgaactgc gccgccttgc cagccagatg gcacgcaacc
    12721 cggaaggccc gcaacggctg aaggatgacg caatggatgc ggttcttgct gccgctgatg
    12781 ccgggtgttt tgccacctac atagagaaac agggcggcgt acttgttcca cgtaaggact
    12841 acctgattcg caccgcctac gacctcgcag atgagctgaa cgattacggc gaacagagcg
    12901 tacagattta cgggatctgg tcgccactca tcggggaatc ctcccgtgtg tgcacgcatc
    12961 cggacaactg gaagctggta agacgtaaac cggaagcgga agacagcgcc cgcgaaaatg
    13021 gttttgacct tcagggcggc cctgccgccc cttggactcg tggcaataac tgtcccggtg
    13081 acgaaaaaac agccggaacc ggcacagaaa acaggcagcg tgggtcgcgg ctgacgcttc
    13141 cggaaagcgg cctggcgcac tggttgcaga cgttaacccg tcacgagcgg aaacagctca
    13201 tcagacagct taaaaatcag ccgcccacca cggataccgg acaaaataac accagaccag
    13261 catcagacac ccctgtcagg gtggaggttg tcgtgcctgt cgacaccacg accatcgcac
    13321 aaatcacccg cgaactcgac gcgctgggtg tgcagctgcc tgacgcagcc attatttccg
    13381 tgagtaacgg tgcccgcgtt cgcccgggcg acgggcgtat cgcgtactgg agcgccacaa
    13441 cccgccgtat cgtcatcaca caggagtcgt cagcggaggt aagatcatga cgcgatttca
    13501 ctgcaccatg cagcgtcagc agttcgacgc cgttatgcgc cagcgcacgc cagccatgac
    13561 actgagaggc gtagccacaa gaccacagcc cggggacgaa ctggaaatca cggtggccag
    13621 ccccgatcgc accaccacaa ccaccatcac cgacatactt aacaggcgac cgggcagtga
    13681 atatcagagc tggaccattg ccgttcgccc acgccgttcg ccggaagaac cggagccgct
    13741 caccccggcc ggaaaaatca tgcagaagta ccacgaagag caggcccgca aacacaatgt
    13801 cgcgctggcc agaacactga ctgccgtcaa tcgcaaggcc gcactggcgc gaagggtctt
    13861 ttcatcgcag cacccttcat tcgccagacc ggcacaacac aggagagatc cacaatgaga
    13921 gagcaattac cagacaccgt gccagtacag gaaatcaaag aaaatatcag gcaacaactt
    13981 cacggatttt atattactta tgatttgtgg ctgaaaaacg gagcaaatcc agacggaata
    14041 ttttcgcgaa actgtggttt atgcgccagc ctctgggatt atctcgaatt aacgggtgct
    14101 gataaagaag ccgcactgga gctattgcac attgatttca gaaatgccgg gcgaaccgca
    14161 tagcggtgag tctgtcaggg cgaggaagcg gaagatccac cgccccacag aatggcactt
    14221 acgcgccatc actgagcaga atatccagtg ccatttgcct ttcgtctggt ttaaggcgct
    14281 ggagcaatgc tttaacgaca ccatcagttt gcaggtcact gggtgaaatc acgcgaagat
    14341 cggaaagatt gagcgaaaaa cgcatattac agtcagaatt agtgcaggtg caataaacta
    14401 ccgccagcgt ttttgcgtca tcctgccatg cagttttgcg tatagccgcc cggcagtgac
    14461 acgccggaca ttcaattttc atcacacccg ccataacccc cccacaatct gaccggttgc
    14521 aggggtaata tagtcttttt cgggttattt ttcatcagcc agcgcagaca ccacattttc
    14581 cacatcttcc agcgtaaaat taaggtgtaa cgacgggggg atttcgggat cgctgttcac
    14641 agtctgcatg aatttacggc agacaggtgt cacttcatcc cgtccatagg ttgtccttgc
    14701 cttatcggga tcgccaagtc cgctcgcatt ttgcgggata ataccggcaa gaccagccgg
    14761 aaagcggtgc gccgtaagaa catcctgagc gctgatattc ttcacatttg aaaactcatc
    14821 tttttgctga atatccccaa ccggaataat tttaatcccg tccggttgcc cttttgggat
    14881 actgataaac atgttacgga aattcccaag cccgttaccg ctctctattt ttttacgtat
    14941 agcttcttca gcctcaacag acatattcgg gtcggacgcg tacatgatga accccatatg
    15001 cgcaccgttg tgatagtagc gacgtctgaa aatggtcgcc tcactgttca gtaacgctga
    15061 atgaatacca ccgatataat caggcagtcc ataaatctgc tgtcgcggat catgcatacg
    15121 taaaaacaca acatcagacg gcgaatagac cagcggctcg ccctcctgta acacgataaa
    15181 atcaagttgc ttgctgcgac gcagatacaa agacggtaat ggtgccagcg ccaccacccg
    15241 tccccacccg ttacggacct tcagaatggc cacatcacca aacagcaggt agtcaaagac
    15301 agccatttcc atctgatcgg tcgtcagtcc acccccctgg tagctggcac acaccatatt
    15361 tttgcgtgca tacagtacgc cgccatgctg ggcgttaagg ttcggcaact gcgcgagcgc
    15421 aagtctgtct attggtagtg tccagtagtt gtagctaccg ttataccaga cgttgatgta
    15481 ttccgtgccg gtagtcagta tcggttccgg ctccccaaaa gtgataatat tgccgcgtcc
    15541 gggagtaaaa ttttctgtct gtgcccgttt acgcaggcgt gcgcttttac gtttactcat
    15601 gcagccttcc cgaatgccca caccgagggg cggtcatatt catagtcgag cggttcatta
    15661 cagaccgcgt gcgatatggc aaaaaatacg tccgcgtgac cggtcgtttc gctgcgttcg
    15721 gcggcgaacg tcattgcgtt ctgactggca gtcattacac ggcggatggc cataaatgcc
    15781 agcgggatat cggcacgttc ttttgtcacc tcatcaaccg catctttcac ccactggata
    15841 cgtcgccgct caatgaggtc aatcatctta agcaccagtt tatttttatt atcaacactg
    15901 tagttaatcc ccatcacctc acgcggtgcg gagcgtacca gttgatcata aacaccccgg
    15961 ccaatacccg tgatatccac cccgatatgc gtaaaccggt agcgtttcat taattcttca
    16021 atctgcttaa cctgccaggt gaagttaagc cccacccact ggtagacagc cagcacgcga
    16081 aatttttctt caggctcaac aggcggggcc acaataacaa atgttgagtt atcacccgtt
    16141 cgtgacggat caaaaccacc ccacacctca cgattaccaa aaggccgtaa tgcggagggg
    16201 tcgaagtcct gccagatgcc gaactcaacc tcacattttt caaggtctga gaatttaaat
    16261 actgtgtcgc gggagtcagt gaactcacac atgtacagca tggcaaacgc cgtggcgctg
    16321 tagcggttac gcagcttctc aatgttaact ctggcactga gtcccttagc gcacgcatcc
    16381 tccattgtga tgacatagcg ccagatcccg tcagggcaga cctgtccacc tttacgcaat
    16441 tccttgctgg tcgggaactc cgccgcctta cgtttcggat cgtcgccctt ccattcatcc
    16501 cctgtccaca gcctgtaagc ctggtgcgtt ttagcgctgg gggttgaaaa ataggtaata
    16561 cggaattcgt catgcgtcgc catcgctgac gccacttcat gcaggcgggc aaatttcggt
    16621 atccagaaga cctcatcccc gtacaggtcg ccactgtagg actgcgccgt attgggattg
    16681 gtcgccagaa aacgcagtat cgcaccattg ctgaggcgaa tcgggttgcc tttaagctcg
    16741 acaccaaaga attgctgcgc aatactgaga atatagaaac ggaaaacttc cgcctggggt
    16801 ttactggcgg aaaagaatat ctgattcttg cccgtgagaa cggccttttc aaacccttcc
    16861 catgcaaaat agtaggtaaa tccttcctgt cgccctttca gaatgaagcg gaacagttcg
    16921 tcgccgtgct cacgcacata ttgctgatat tcaaacagat gaccattcgc tttttcatca
    16981 aatatttctt ttgtcagtcc gccgacatca tttttacgta gtcgcttcct ttttttacca
    17041 ccttcgtcat tcccttcttc actgctaccc tgtcggccat cattgaccac cacgccggta
    17101 ttgaccatcg cccggatctc ttccatccgt tcagcatgtt tgttcttctg cgccatcagc
    17161 ctgacgtgct gggatatcag gaagcgcagc tcttcaagct caagctcgct tttaccatcc
    17221 ttgcaggtca gtcgttccac tcgccgggcg acagccactt ccaccgactc ggctggtaac
    17281 atttcattcc attgcccccg gacagcccag ttataaacgg tacgcacagg aagccccagc
    17341 gtggccgcta tctcttttgg tgtactgaat ttcagataca gggcgcgggc agcgtccctg
    17401 acttcttcgg tatatttcat tcgcccatca taaagagcaa taacactctg aaatatcgtt
    17461 aaattttggc atttttcgca cataaaatca tatacgaaaa gcccggattc agaacgaatg
    17521 aaacccgcga aaacagccgc aataataccg ccagatacat caaaaagcgg taatgaaatg
    17581 gcaaagcaga aatacaccac tgactggata tgcatagcaa ccgaaggcta cgccatcgac
    17641 ggtcgcccca ttacccgcga aatgatccag caggccgctg acaactacag caccgccaga
    17701 tacacagcga tgatctggcc taatcacccc gtatacagtc ttcaggagcg agaatttacc
    17761 cccaatctgg ggcttgtttc cgagctggcg gctaaagaag acgccggaag actgcgcctt
    17821 atggcgaagc tggagcctaa ccaggcgctg gttaacctta acgatcgcgg ccagaagctc
    17881 tttacctcgt gcgagttctg ggagaactat gccaacaccg ggcaaaccta tctgtacggg
    17941 ctggcagcaa ccgacagtcc ggcaagtctg ggaacccagc gcatggaaat caaatcagtg
    18001 gaaaacaaca cacagggctc acgtccgcag tatgccgccg ctggaaatat tgaaatgttc
    18061 agtcttgggc gtctgacgct ggtcgctgac accccaacaa accatcatga ggataaatcc
    18121 atgactaaag aacaggcgaa ccagttgcta gcccagattg tcactctgac ggcaaaaatt
    18181 gacgcgcagg cgaacacgca accacaggca cagcaacccg caccggcgca gcccacggca
    18241 tccgcaacag cagacacaca gccagtaacc ggggatttca gcgctcacgc taatcacgtt
    18301 gcggagctga gtgaaaagct ttccgttgcc actgacacac tggccatcaa cccgaaagac
    18361 gacacggcac gccagaacta caccaccgcc gtgacggagc tacagaccgc acttaacacc
    18421 tttacggtgc agccacagga tgctgaaacc gcaaaactcc aggcacgtat cgccgcccgt
    18481 aatggcggac aacagccagc gccagccgca gcgcagaatt tcagtacccg gaagacggaa
    18541 aaagatgatg aggccgacgc cgccacactg acaacgctta ccagtgcaat cgaaaatctg
    18601 agccagcgtt tttcggtcat tgaaagccag cgcacaccga cgccgggcgc agcccccggc
    18661 ggacaacccg cacagtttga gccgctgtaa gcggtcgttg agaaaaacag acaaaaggac
    18721 caaccggaga aaaaccaatg gccgcacaga tgttatcacc acaggcgcaa aagtttttgc
    18781 gtaattacgc catttcgatg gcaaaagcca atggcgtatt tacagcacaa cagtttttta
    18841 ccatcagccc accacgtgaa accctgctac gcgatgccct gattgagaat gccgacccgt
    18901 tcatgggcaa gatcaccgtc atgctggtcg aacagcttgt cggccaggta gtcagtaccg
    18961 gaattccggg gctgtatacc gggcgcaaga aagacggacg cttcaataaa gcgctgggga
    19021 gtgaaggcaa cgagtacaag ctttacgaag tggattccgg ttcattcctg gactacacca
    19081 ccctgaccaa ctgggcaaac gcaggcaccg aaaacgagtt ctataaccgt cttcaggcgt
    19141 tcttttacaa gtcagtcacc aacgacctgt tacgcgtggc actgaacggt acgcatgccg
    19201 cagatgaaac caatcctgat acctgcccga acggggaaga tattcacccg ggatggcatc
    19261 agattgttaa agcgcgcaca ccaaaacaga tcattactga caaggtcgta ttgaaccgta
    19321 caggaaccgg ggctgatttt acgtctgttg atgctattgc cgccgatgta attaatacct
    19381 gcattccacc cgaattccgt caacacccgg atctggttgt actggtatca cagaacatta
    19441 tcgcagcaga tacggtcagt atgctgaacc gcatcgaccg cccgacagaa aaagtcgccg
    19501 cccagcttat caaccgtgag atcgccggac gcaagtcatt cagtccgcca tttatgccgg
    19561 ataaccgcct ggttgtcacc acgctctgga atctgcatat gtacttccag cgcggaacac
    19621 agcaacgccg tgctgaatgg attgatgacc gcaaacgctt cgaaaacaac tggctgcgta
    19681 tgcagggttg cgccgtggaa tatgacgcac tctacggttc atttgacaac attgaactgg
    19741 cgggactgcc atcacctgaa catgcgaaac caaaccaggt agcataacgg tctgacgtat
    19801 gcagactccg gcgcaaaagt tcagagccag actggccgcg cagcaggcga tggaacgcca
    19861 cgaggcactg accgtacgcg acagtcttca tctgcaactg gcgcaactgg aaatcgactg
    19921 cagggctgtg agcagttgcg ccaccaacgc cgatcgtgtg gcattcaagc gtgaatccct
    19981 gctgccgcgc tggatgccga cgattgaggc gtggctgcaa agcggcgagg agtacaaata
    20041 catcccgttt acctggtgca tcgtctggct gtttgatacc ggcgaaatgg acaaagccct
    20101 ggaatgggcc gatatcgcca tcgggcagaa tcaacccatg ccaccacgct ttgaccgtga
    20161 tttgccaacg ttcgttgccg atgaggtcat gcagtgggca cagattcagt ttgataaagg
    20221 cctttccgtc gagccctatt tcagccgcgt gatgagccat atcgaaaaag actggacact
    20281 gcacgaagag atagttgcca gatggtacaa attcgccggt cttaacctgt tacgggacag
    20341 cgatggcaaa ccgacaaccc cggccgccat ttacgacgtg gcgaaactac gtagcgccga
    20401 tgagtggatg gcccgtgcgc acagcaccac ccccaaagca ggtgttcgcg ccatacgaaa
    20461 caaaatagcc gcccgtctgc gcgcattacg ggcggtataa cgaaagcccc acggcttcag
    20521 gcgggcacgg tgaggatgac acgcacaagc gtcagtcatg ccgcagaacc gttcgcccga
    20581 cctgacctac acagagaatc agtcatgaac ctgaaaccag gctacagcac agaccagaac
    20641 acagacactg ctacaggcat gcttaaaaat gatggcttct ggccggatct ggtcatggct
    20701 gattttcgca gaagccgaac tatcccactg gatatccctg catcactggc agaacaggcg
    20761 ttattgtctg ccgtctttga ggtgaacacc gcactgaccg gtgtgaaatc ccgctacgta
    20821 gtgcagggaa tagcgcaggc ggcggacgtg gaaggcccgc aacttaacgg cgaaagcgcc
    20881 ctttgcgcac agtacaaaaa agcggtcttt gcccgcgcca aagccgatct tctcggtgag
    20941 tttacggcgg tgaacacccg ccacgccgac agcaatatcg acagtaatga cacccgcgaa
    21001 accctgcttg ctgaagcggc ctttgtgctg cgtgctatcc agggcgcagg ccgtgtggga
    21061 gtgtacatct tatgagccag ttcgacagcc tttatgcata tctgcggcag gtgtttgaca
    21121 tttccgtgtt tgacaatacc ggctttgatt cgtggatgag tgatattcgt cttttgcccg
    21181 cagagaaaga catggggctt ggacagataa gaatgggtat tcgccagtac aacgccgtgt
    21241 tcgactggca gcgctggcca tggcgtcagg ttgaccctga tttgctgtta tgcgccctgc
    21301 ttgtctggcg ggacgaacag gctaatgact actacagcca gcttgagctt gatgcgccgg
    21361 atgtcaacat cgaacccacg gacgacaaca ttgcagaagt gactgtcagt atgccgctcg
    21421 ctgaccccat cattctgatc ccggacgagg ccggttttat catgatgggc ggcatccgtt
    21481 accggctgga taagccagag atctgggtgg cagaaaatat cactgtatcc accatcaggg
    21541 cagggaaata aacaatggac atctccctgc acggcgaact taaccggaca cagcttaagc
    21601 agctgcagac aatgctggcc aacggtgagc tccccccggc aaaacaacag cgtctgctgc
    21661 agcgtatcgc aaaacgcggc gttattccgg ccgcaaagcg ccatgttcgc gaacaaaaga
    21721 atgttgatgg cagcagtttt gcagaacgcc gctatggcaa cagaccgctg ctgaaggggc
    21781 tccctaaatt tctgaaagtg cacgacatgc catcagcgaa atcggtgcgt atatacgcag
    21841 caggcaggag ctatcgccag ccccacaacc acagggaaat cagcgtgggt gcagtgggat
    21901 atatccagag ccatggcgtt acgttcacag tgaacgcatc gcagctgaaa accgacgcga
    21961 tggaaaaagc cagcaaggaa ccctgctccc gccgtcaggc gataaagctg cgcaagctgg
    22021 gctatacggt gagcggcaaa aaaaggggga cgcgccgcaa accatcaacg gcagatatcc
    22081 ggacgtcgct cacgaaaggg caggcaggcg tgattatccg cagcatggaa ggcataccat
    22141 ccaaaagctg ctggacgata accatcccac cccgtcagtt ccttggtgtc agtaacgagg
    22201 aatttgcacg gatactgtcc cgccagttgc agggcatcaa ttatggctgg cagacaaagc
    22261 cgcaggacat taagaggaaa taacccatga cgtttccaca ggtcacaatt aatcagctca
    22321 acacccacag cggaagcaag cgtgggatcg cctgcaccct gctgatggtg ggcgagcata
    22381 caaaagccat tctcccgaca ccggttacag cacaaaccga tcttgactcg ctgttaggaa
    22441 caaaggattc attgttacgc agtaacgtgc aggcgtttct ggataatgcc ggacaaaatg
    22501 ccatgatctg gctggcaacc gtgcagacac cccagccttc tggtcagacc cgaacgtggg
    22561 ctgatgtggt tatggatgca caggccaccc tctcggtgga aggcgttgtc gttgttcgcg
    22621 ctaacgcctc aggtaacgat atcgacaaag cgcagcaact gcgcagcgaa ctcaataaca
    22681 ccttccagcg ctggacatgg tttatcctgg ctgttcgcgg ctgcggaaca ggcgagaagt
    22741 ggtcagaata cgtcaccgtc atgaccggac tacagaaagg cattgccgct tacgcggtgc
    22801 agcttacccc gatgctgttc ggcaatgaac cggggatact ggccggtcgc ctttgcaatc
    22861 catcagtgac gattgctgat tcccccgccc gtgttgcaac cggcgcactg gtcaatatgg
    22921 gccgcaacga taaaccgcag gacagcgaca agcgcgaact ggacattgcc accatcaggg
    22981 ccctgaacag ggcgcgtttc agcgtaccaa cgtggtatcc cgactacgaa ggttactact
    23041 gggccgacgg cgtcacgctc gatgtggacg gtggcgatta tcaggctatc gaatatctgc
    23101 gcgttgccga tgaaatggcc cgtcaggtgc gtttgctggc tatcccaaaa atcgccaatc
    23161 gctcactgaa ctccaccccg gcaagtgtgg ccgcgcacca acagcttttc gcgaagccca
    23221 tgcgcgacgg cgcaaagagc ctgaaaatca acggcacggt attccctggc ctttgcatgt
    23281 caccgcacga cggcgacgta aaaatctcat ggccggaaaa agacaaagtg cagattgcga
    23341 tcgtggttcg cccgtataac tgcccgaaag aaatcaccat cagcatcatg ctggacgaaa
    23401 gcggagagta acccatgaac accgaacgca tcagtggtat gagctttgac acgtcattta
    23461 atggccgcat cgttcacgta aaaaccgcca cccttgatat taccgacaac accaaagcca
    23521 ttcaggagcg cggtgtacct gatggctggg tacgcggcga cgtggaagcc ggcggtgaaa
    23581 ttgaactgga taccgttaac ttccagcttc ttggtgaagc cgcgcgtgaa gcagggagct
    23641 ggcgcgacat cgaagaagcc gactttctct tctttgcccg tgcagcaaag acagaactca
    23701 gggtggaagc ctttggttgc aaactgctga tcagtaacct gctgaatatc gacagcaagg
    23761 gcggtgacaa cataagccat aaaatcaaat ttatggtgac ttcgccggag ttcgtcaaaa
    23821 ttgacggtgt tccggttctc agcgccaccg atgttcggga catcatgaac caataggaaa
    23881 gacaatgcaa agcacccccc acaactggag cgactggctg gcgataatca aagactggtt
    23941 acgtggtgat atccctcttg acagtctgct gatgactgcc gtcattgcag cactcagagt
    24001 gttttacaca ggccgcagct ggcgtcgtct tttgcttgag gtgccgttgt gttgcctgct
    24061 ggccgtggcg gcattcaccc tcattaagcc ggtccccgtc gcatggctga ccgaagactg
    24121 gcgcgtcggt atcggtgccg caattggcct tattggtgtt gaacatatcc gggcactggg
    24181 tgtgttcatc acaaagaaat ttgcagggaa aagtgacgaa tgaaaatttc agacaacggg
    24241 ctggccgcac ttaaacgcga agaaaactgc aaactgaccg cttaccccga tccgcgcggc
    24301 gtatggacca ttggtacagg tcacacagga aaggttgatg gtgtcgcagt tcacaaaggc
    24361 atgaccatca cccaggacac cgccgacaga ctgctacgcg acgatctgtc atgggtggag
    24421 cactgcatcg ctgaacgggt aacagttccg ctgaaccaga gtcagtatga cgcgttgtgt
    24481 agcctgattt tcaatatcgg cgctgacgct tttatcggtt ccaccgttcg ccgtcagtta
    24541 aacgccggca actacaccgc cgccgctgat gctttcctga aatggagccg cgcaggcagt
    24601 aacccgacaa ttctggcccc gcgtcgcgga cgtgagcggg caatgtttct gggacagagg
    24661 taaggccaca tgaaacgcgc aacactcgcc tttatcgtct tcatggtcgt cgccgtggcc
    24721 gtgctcggct tcacagctga ctactggcac aaccgcttca ccagggcaga aaaagcccgc
    24781 cagaaagccg taaatcttgc cgacacccga cagcagacca ttgacgacat gacactgcgt
    24841 cagcgccaga acgccgcgct tgatgcgaaa tacacaaagg agctcgccaa tgcaaaagct
    24901 gaatccgaaa agttacgcgc cgatcttgct tctggccgtc gccggttgca gctccacgcc
    24961 gtctgtatgc ccgccgccac gcgtgatacc accgccaccg gctcaactga tgcaaccgcc
    25021 gcccgactta ctccggacgc tgaacgggat tatcagcatc tcagagccga agcgaaagcc
    25081 gtcgttgcac aagtgaacgg cctgcaacag tacattactg aacaatgtca ttaaaaaatg
    25141 gcaccactca ccggggaaaa tagcatgaaa gacgaaaata ccaccatcat caccctgacc
    25201 gtcgcaggcg aggacattcg cttcgccccg accgaagctg tctacaacaa gttcgtgaat
    25261 gaaataacga tggataacaa ggtggcgcca gccaaaaact acctgatgcg ctgtgtacac
    25321 ccggaagaca aagagaaact ggagaagctc attcaccgcc cgggagcagc ccttcagatg
    25381 gcagcaaaac tcaatgaaga ttttgccgtc aatctggata tcaccgtaaa aaaataaagg
    25441 cggcactgtc cggcattgag cgcaacggct acgcgcaata tctggtatta cgccgccact
    25501 ggctaccgga tggcgaggac acaccggaag acattgccgc tgcggtctgg ctggacaacc
    25561 gttatttcga aaacatgagt attgccgtca ataacggcat tgcgagagca tttaaaggtg
    25621 ggtaatggct gctgaacttg attttacgct aagtctggtt gataagctga cgagaccctt
    25681 aaaacaggcg caatcggctg tgaccggatt tgctgacaag gcaacagccg actttaaacg
    25741 tctcggtttt ggtgtggccg ggttatgggg ggctgctcag ggaataaaag gactggtcaa
    25801 ccccgcccgc gacatggaag ccgcgctggc cgaagtcagc tcgctggatg tggcaaataa
    25861 aacactcgat cagctgcgta aaacttcaca gacgtttgcc attaactacg gcgaaagcgc
    25921 cagcaatttc gtccgcagcg cctacgatat ccagtccgcc atcgcaggac ttcagggtga
    25981 cgaactgcca aaatttactg aagcatccgc cattctcgcc aaagccacca aatcagacac
    26041 ggccaccatt actaactata tgggcaccat gtacggtgtt tttaaaaata ccgcagagaa
    26101 gatggggcgc acgcagtggg ttgaacaaat tgccgggcag accgcaaccg ccgttcaggt
    26161 gttcaaaact accggtaatg aaatgtctgc tgcctttact gcgctgggtg ccaacgcgca
    26221 ggcaatgaag gtatctgccg ctgaacagtt cgccgtactg ggacagctcc agtccaccat
    26281 gtcaggcagc gaggcgggaa ccaaatataa atcattcctg gcggggattg ggaatgcgca
    26341 aaaagtgctg gggctgaact ttaccaaccg ggacggcagt gccagaagca ttaccggtat
    26401 catcgacctg attaaaggaa agtttggcga tctgtcaaaa gtggcagatg cagacctgct
    26461 gaaaaaagcc tttggcagcg atgaagcggt gtccatgatt aaactactgg ccggagatgt
    26521 tgacgggctg aaacggaaca tcaatacatt aggtaacatc aaaggcatgg ataaagccat
    26581 cgaaatggcg aaaaagatgg ttgacccgtg ggatcaggtt aattctttac tggagcaggt
    26641 tcgcgtcagc attggtctgc ggctcgatcc cgtctttgcc ccgttcctgc aaaaaatcat
    26701 caccggcggg aaagccttca tgaaatggct ggatacgttc cccaatattg cccgctggct
    26761 gggatatatc actgcaatga ccctcgcctt tgccgcagca ggggcgcttg ccagtatcac
    26821 gatgggggcc ttcggcttta tcatgaccgg gctcagcgga attgctggcg tgctgaaagg
    26881 cgcatggaaa ggactaatct tcaccctgga tttattacgc ccctccctgc tttcaacccg
    26941 tcttggcctg atggggctat ggattcagga aaaagccgtc tgggtgtggt caaagctggt
    27001 cgctctttgg gcagggttct gtaaagcagc gatcgtagcc tggaacgtag tgttacgtgc
    27061 gggcgcggtt gcaatgcgcc tgtggggcgt ggcgacggca ttcgcaggcg gtgcgcttca
    27121 actcctgata agtcccataa ccctgattat tctcgccatt gccgcacttg ccgtagggat
    27181 ctgtctggct atcaaatact gggacagtat taaagccgcc atcatggaca cggaagcttt
    27241 cagtgccctg ctaaaagtca ttaacgctgt ggtgggctgg tttgatgatg catggaacag
    27301 cattaaagcc ggatgggaca atctgacgaa ctggtttaaa aatttctcgc tggccgacac
    27361 attcggcaat atcacagctg gaattggcaa gctctttgac ggcatatggg attccattaa
    27421 gagcgccttt accgggacat ggaactggat agcagagaag ctcaacaaaa tccccggtgt
    27481 caatatcagc accgcacaac agaccgtcgc accgacaccc gccccggagc ttatcactgg
    27541 caaccgtagc gccgccatcc agggcgggcc ggtaagtcac cagataagca acaaccgggg
    27601 cgacaaagtc accaataaca acgttaagca ggatatcaca ctgcacgttg acaccctggc
    27661 gacaccggga caactggcgg aatacagcga actggcagcg gggtgacaaa tgagtacgaa
    27721 atcactttat atcgacctgc tcattactga cggcgatctt acgttcaact ccgccagtga
    27781 gccggtcttg tgtgacaacc gccagagtat tgctcaggac atgattcacg ccctgattga
    27841 aagtggtctg ccctgtcgtc tgattgcgga gaacagcccc acactcaggg ccgacctgtt
    27901 cacgcaaatg gtgatactcc tggaggagga tgaacgcctc attcccggct ccgtatttat
    27961 tgacgaagag caacgcggcc agctccttgt taccgccgat acctacgatt ttggtcagtt
    28021 aagccagggg atgcgctatg cagaataata tcgacttcaa aaacatactg cgcgaaagcg
    28081 gaatgccggt tgatgaacag accgtacgtg acaccctgca acaggtggca gatgatgaaa
    28141 aactcatcac caatacgtcg cgcatgtcgc cattctggcg gctgattcag ttgctggtta
    28201 tcacgccgta tgtgtggatt gttgacacac tggctgacaa tgtactgcat aatctgtttc
    28261 tgatgactgc cagagggccg ttcgttgacc tgttcgctgc cgccctcaag cttacccgca
    28321 aggaagccac gcgtgcgatc ggtaaaatca tttttaccaa aaacaaccag aatgacagtg
    28381 tgaccgtacc ggctggcaca ctgattcaga cggagcgtat taacggtgtg atttattcca
    28441 tggcgacccg cgagcaggta attatccccg caggcacgca aagcaccctt attgatgtga
    28501 tcgccaccga cagcggtacg gcattcaatc tggcaccagg ctattaccag atactcccca
    28561 aagcgattaa cggaattgcg tctgtacgta acggtgataa ctggctgacc ataccgggcg
    28621 caaaccgcga aagtgacgac gaactaaaag atcgttgccg taaccagttt aaccttgccg
    28681 gcagttatca cactgacgca gtttaccgca gcctgatagc tgcacaggcc ggactgacca
    28741 tcgatcgcat cttcttttta catgacgccc cgcgaggacc tggtacaact aatgcctacc
    28801 tgctgctgga taccggggtt atttcgcaac cctgggttga cagcgtgaat gattacatca
    28861 tggcacaggg acatcacggc cacggcgatg atatgcgttg ttttgccatg ccggaaaccc
    28921 gccacgatct ggcggttacg gtgtatgttg aaaacctttc caatatcagc aaaaacgata
    28981 tcgacgccct gaaatcaggc actgaaaacc tgattcgttg tgcattccgt gaaaacagta
    29041 actacgacgt aacccgcaca tggccataca gtcgcttttc attctcgcaa ctgggacgcg
    29101 aactgcatga cacctttcct ctggtgggat ccgtgacgtt ttcccttgag gatatcatca
    29161 gcgatctgaa cgtaccccgc ctgaacacac tgacagttga ggtgaagaat gcctgataaa
    29221 tcaccggaca ttaagttgcc gtcatggctg aatcgcggcg atgtgctccg cctgaaaaac
    29281 acctttatcc gtttctgggg aaaggtgcac gactgggtaa catggccact gagccagacc
    29341 gacccactga catgtgcgga atccattctg aacctgacag cctggcaata tgatatagcc
    29401 cgttttgacg gcgaaccgct cacgctgtac agaaagcgtg tcaaatatgc ctttatcaac
    29461 gcacaggacg cgggcagcgt ggcgggattc agggcaattt ttgaacgact ggggatcggt
    29521 tatgtggaga ttcaggagcg ccagccaggc acagactggg atgttgttct tttgcgcctg
    29581 acagacaacc agatctcaga aaatacaaca ttactcaatc agataattcg ccagtacggt
    29641 cgcacctgcc gccgttacca tcttcagata atcacatcca cggattgtgt tatcggacac
    29701 ggatactggc atggcagcta tcactatttt tatgcaccgg agatgaaaaa tgacccagag
    29761 cgctatcact tcagcatttg agcgcctgaa agcgcaggaa gccgcaggcg gcaaccgtat
    29821 catcattgat cagtttgtat ttgcaaatat cccggatctg aacattgccg acacgccgtc
    29881 tgaaaacgaa ccattgcccc ccgatgcaca gatagttcac cgccagaacg ttgatcgcaa
    29941 cggaatggta aatgagaaca ccgtcgccta ctccgttacc ctgccggagt ccacgggtga
    30001 ttttactttt aactggctgg gtctggtaag cagcaccaca aatacacttt gtatggtggt
    30061 atatcttcac cctcagaaaa aaataaaaac cgcagacggc aaacagggta ataccctgat
    30121 ttattctgaa attatggaat acgccggagc cagcgccgca accggcatca ccacgccagt
    30181 aagcacctgg cagattgatt ttacggcacg gcttcacggc atggacgagg ccacccgcaa
    30241 agcggcgctg gatatctacg ggcctggcct gttctttaat aacgccttta aactcaccgc
    30301 tcaggctacc gggcaggcga ccttcgcgcc gggtattgcc tatctgcgcg gattacgtgt
    30361 ggagctggat gaacaaggca cactgacata cgccaccgga atttcacaaa ctgtttatgt
    30421 tgacgcggca ttaaccggta ccctgaccgg ggaaaataaa gccatattca gcctgacgag
    30481 tcagaaaaca gccgactata ccgacagttc aggatttgcc cattatgtcg aacccatcgc
    30541 cacaatatca gacagtggcg agatcaccga tatacgaaaa acccgcaaac cattaaatga
    30601 ccgccttgat ggcgaatacc tgacccgtaa cgggaacctg aaagaaattg ctgacaaagg
    30661 tgaacaggcc cagtctgatg ccagaaagca tatgggcctc ggcgacagcg ccacccttaa
    30721 tgttggcacc gcgcaggaca ctgtagccgc aggtgacgac atccgcatca caggagcgct
    30781 gcaaaaaaat cagaacggag cagatattcc cgataaaccg ctgtttatcc agaacgttgg
    30841 tttacaggaa acggtaaaca aggctgataa cgctgtccaa aagacaggcg ataccttgtc
    30901 cggtggactg acttttgaaa acgactcaat cctcgcctgg attcggaata ctgactgggc
    30961 gaagattgga tttaaaaatg attcggatgc agacaccgat tcctacatgt ggtttgaaac
    31021 ggcggataac ggtaatgaat atttcaaatg gagacaccgc ctcgctggcg gccgggttaa
    31081 agacctgatg aatcttaaat gggattcact aaatattctg gttaatgccg tcattaatgg
    31141 ttgtcttgga attggtacga cgaatgcgtt aggtggaagt tcaatcgttt taggagataa
    31201 cgataccggg tttaaacaaa atggcgatgg tctgcttgat gtttatgcga atggtcagcg
    31261 agtattccgt tttcagaatg gtgtgactat tgcttttaag aatattcagg ccggaactgc
    31321 cagaaaattc acgttatcca gcgccaacaa ctccacgaaa aatgcagcgt tttatttgtg
    31381 gggtaatcca tccaggcctg ttgttgcaga gcttggtgat gattcaggct ggcatttttt
    31441 cagccagagg aatacagata acagcatcac gttcgccgta aacggacagg taattccgtt
    31501 aaattacgga aacttcgatg cccgctataa acatcgaact gagggggtac aggatgtgcg
    31561 gtatggccat gaaatgtatt acaaccctgg cagcaacacc gtttcatgga cgtttcgctc
    31621 accttcggga cacgggctgt cagggatatc gatatcggat accggccgta actcagcgga
    31681 taacgtcaac ggtgtgtatt accgaccgct gcaaaaactg attaatggca cctggtataa
    31741 cgtagcgagt atttaatatg atgcacttaa agaacataaa agcgggtaac gctaaaacgc
    31801 cggaacagta tgagttaaca aagaagcaca gagtcatctg gctttacgct gaagacggga
    31861 aaaactggta tgaggaagtg aagaactttc agccagatac aataaagatt gtttacgacg
    31921 aaaataatat tattgttgcc attaccaaag atacctcaac gcttaatcct gaaggtttca
    31981 gcgttgtcga agttcctgat attaccgcca accgacgtgc tgatgactcc gggaagtgga
    32041 tgtttaagga cggagctgtg gttaagcgga tttatacggc aggcgagcag caacaacagg
    32101 ccgaatcaca aaaggccgcg ttactttccg aagctgaatc agtcatccag ccgctggaac
    32161 gcgctgtcag gctgaatatg gcaacagacg aggaacgcac acgactggaa gcatgggaac
    32221 gctacagtgt tctggtcagc cgtgtggaca ctgcaaaacc tgaatggcca caaaagcccg
    32281 ggtgaaaacc aaggcccgaa cgccaggcct tttgcgaata caccggcatg ccagagtgga
    32341 aacaaattgc gacgcaccag tccgtgcaca tttctgaccg ggcagctatt ccagaaaaca
    32401 gcagacaaga gaggtacaga tatgtggttt ccggcaaaac tgatgcttcc gacaggtaat
    32461 atcaccccca taacatgttc agctatggcc gtacatccgt gggatgttga agcggggcac
    32521 attacgccag acggtgcgta tttaagcccc gtcaacgcaa taaaccacct taacagcaaa
    32581 ctcccgggcc tgaccgaaaa cagcgatatc gccatcataa tggtaacagg cagcaataat
    32641 aacgatttta ttcgtcaact gtcagcactg gcagacacac tccctttacc cactctgacg
    32701 cagacgctaa gacgcgcaag aacacagctc acccaggcaa tcaccagaat gcaaatcccg
    32761 gcgacaccac agaacggact acccgctccg caaccactta ttgccagcac actacagaac
    32821 agcatcagta accacaccgc gcttaacgct atgaaggccg aagggctgaa ctctgtcgac
    32881 tcactgaaaa acgcgttatc cggttttcag gcacaacgtc aacggttgca gaaacagata
    32941 atcgctgaac tggcaggagc aggcgataaa gtggcaaaag catttgcgtt cgtgcacacc
    33001 ggcgacgccg ttttagcccg ccgggaaatg atgaaaaata ttccgcaccc cacagcttca
    33061 ctgacatatg cacacctgtt cgcaggcgat ctttccggga tgctgaactg gataacgaag
    33121 gcagaaaatg agccagaaca cgacaacgcc actgctggcc cttaatggtc agataatccc
    33181 gctaaaacgc ttaagtgtca gcgtgaagct gaccatcaaa gacaaagacg cttccggaaa
    33241 atcctcatca acagcgactt cagaacaggg tgtaaaagcc aaggagctgc agatttccgg
    33301 actgattccg ttcacccagc cggaagcact cacacgctta tttcgtctgg ctgaagccag
    33361 agccgctaac ggtgcacaac aggtttaccg catcgccaac atggatgcca aagcggtcaa
    33421 tatgaagcaa gggatttttt ccggtgccgt gggcgcaact cctgaaacag gcctgatggc
    33481 atggaaaatt gacttcactc tgaaagaaaa actcagcagt gccgaaaagg cgacaggacg
    33541 cggcccggct ggcagccaga gtgctgaaca gcacgcacag gccgtcaaat caggtggcaa
    33601 aggcgaaaaa aacgaagagc atggctggtt ctggaaaatg tcagacaaac tcaataactg
    33661 gataggcccg gcaggcaatg aaaccaattc gtgatctctg cattaacggc aggcgttatc
    33721 ctgtcgtgga agaaaacatc atgttgcgtc tcaacggcgc gggaaccggc tttatcacca
    33781 ttaacgccgc cgacaatact cccccgctac gcggcaaacc cgtagagctg tccatcggtt
    33841 acaatgatca gcctgtaaag tggttttcgg gatatgtgga aagtgacagt tgtacaggca
    33901 gagggctgca caagctgatg gtgcgcgaag ctgctgctat tctgcaatac ccgctcaaca
    33961 tctccatgca gcatccgacg ctgaaacagg tcgcaggaca cattgaaggc aatacaggtt
    34021 tacgcataca actcccgaag gcaggctaca ccaccactcc ggtccctcat ctgacccaca
    34081 atggcaacgg ttatcagctg atggccatgc ttggccgcat attcagtatc ccggattacg
    34141 tctggtatcc gtcggttgac ggcatcatct acgccggaag ctttgctgac tgccgttttg
    34201 caaaacgccc tgccagattg ccggtcgata tcaccagagg tgatcacgga gcaaatggct
    34261 ggacaataca gacgattccc atgatgcgcc ccggcgtggt catgaacgat caccgtataa
    34321 atcaggtaca gctacagggc gacagcatga tcattagctg gagtgatggc cgtcagtcgc
    34381 ccgtacaacg ccagatcgaa accctttacc ccgagatagg caacaaaacc caccttccac
    34441 gcatgggacg cgtgatctca ccaactgaaa acacaaccca gggggatttg catgatgaat
    34501 ttcgcccacg ctacgccgtc aatgtgcagt tgctcgacga gaacggcacc cccgataaga
    34561 acacaccggt atataacgcc gtccccgttc ctgtgccaat ggccggcaat gaatccggga
    34621 tattccagta cccgccccca ggaaccatag ttacactggc ccacgttgac ggcaggccgg
    34681 ataaacccat catcaccggc actcacgcca gcggccagag tctgccggga ataaagcccg
    34741 gcgaacaact acaacagcaa cgcgcagagg tattccagcg tgtgcataca gatggcacat
    34801 ggcagcgtga aaccgatcag gttattcgtg agaaatccac cgaccgcatc atccagaaca
    34861 caacggaaac gcgcaccagt accactcgta atattaccgt aaaggccaac aacaccataa
    34921 cggtgctcgg cactcataag ctgatgagcg gccacataca gcacatcgca gacggtgact
    34981 atgccgtagc cgccacgaag aaactgatac agcacgccga cagcgctgaa ctcgacgtta
    35041 ctcaccagtg gcaaaccagc acagaaagca ccacccacaa tgtggcgaaa acgctggaag
    35101 aaaagattgg ccggattaaa aaatccgtcg ctggccagat gcaacagatc atcgccccgc
    35161 aggtctggtt tggcagcaat accatcaaca cacttaacct catgctggat ctttgcgaca
    35221 ccgttcagca actggcgcag ctcaccgcac aacacactca taccaacaac gggtcgtcac
    35281 agcccaccaa cagcggcagc atagacacgg tggcatcaat ggcaggcaac ctgaaagcga
    35341 agtacagtac cgtgattaag cagtaagttt acagatgaga agaggtgcac aggcagcccg
    35401 gtttaccact caagaatcat gccacaaaat acagacaaat aagcctttcg tgaaattatt
    35461 catatatcaa taaatttcat tcatcgaacc agacgaaagc ccgtcgcgac gggcattccc
    35521 ccagaaaaac accccccacc agacgcgctg tatcgcattc tgcgacatgc tttaagcaaa
    35581 taaacgcgcg agtccccctg aattcagctc gtaacgcctg aaagcgtaca cgcacgctac
    35641 gtggatattt cacggcatga tgagcccaac acacggcacg ggcgcaagcc ccccgcgctt
    35701 gcactcttta cgataaaaat tttttgccaa ataaatttgt gcaaaacgac actccagacc
    35761 gcgccagcac tggcgcttta caaaaaagcc atctggcaca aagtgtcaaa gattgccagg
    35821 ctttgccaga ttaacgttaa aaaaggatct gacaggaaaa ttaaccatat gaaaaacaaa
    35881 accatttttt gttttcgtca ttttaaagga tctgaatgcc gccaaagtgc cacgcacagg
    35941 aaccacataa cacaatgaaa taaaaggtaa aaacaaaaca ttggacaagg atcgttttgg
    36001 cgcagcgtta cacagtttcg ctgtaaaact attttcaggc gcaagcaaaa accgctcacg
    36061 aaagcagcga caaaagcggc aaaattttaa ctttcgcgga gaaccgaacg ggtgacgtga
    36121 aactttacgt agcagagaag cgggaaatca gtagaataat cgcgggtgcc tttcggctga
    36181 cggctggaag gttaacctga aggccggatg tggaaaggcc ccggaaaaca tttttgttta
    36241 accgaggccc taaccgtcta accagcaatt gataggttag cgcctctcca ctcaggaggc
    36301 aagcgccatg tcgcaaaaat cgctaatcgc catcaccaca ctgtgcgtga cggcaatcct
    36361 catcatctgg atgttgcagg gttcgctctg cgagatacgg atgagtttct ggggagcgga
    36421 gtttgcggcg ttcttacaat gtagacagta agaaaaccgc gacggggaag gcaacttccc
    36481 cgtcaatcgg ttgccagggt aaggccgaca aggcacccta ttctcgcagt catggacaaa
    36541 cgaacccgca atgtaaaagc tgcgggtttt ctttttggtg tcctcaccga cttgaaacag
    36601 tgatcagact ataagacaaa gcccacaatg tagcgggctt actcccctcc cccccactaa
    36661 atgtggacac atcgtggaac gaagagatat aaaacattat aaatcaattg gttattgaaa
    36721 gaaaaaaggc cgcagagcgg cctttttagt tagatcagat tactcgtctt tgggcgaagc
    36781 gttttcgacc cggcttttta acttctgccc gggtctgaag gtcaccacgc gccgtgctgt
    36841 aatgggaata tcctcgcccg ttttcgggtt acgtcccggg cgttgattct tatcacgcag
    36901 atcgaagtta ccaaaaccag agagtttcac ctgttcgccg ttttccagag cgcgacggat
    36961 ctcttcgaaa aacagttcaa ccagttcttt ggcatcccgc ttgctaagcc caagcttatc
    37021 aaacagatat tctgacattt cagcttttgt aagcgccata ggttcaatcc ctcaatgatg
    37081 cctggaatcg ctcttttaat gcctctacac atttggcgac ggtagcggca atctcctctt
    37141 cttcgagtgt acggctggta tcttgcagga tcaggcttat ggcgaggctc ttatacccct
    37201 ccgcaacacc cttaccgcgg tacacgtcaa ataagtttac gccaactacc tgatttacgc
    37261 caactttctt acattcggat aaaatatccg ctgcgggaac gttttctgcg accaccaccg
    37321 cgatgtcacg acggttcgcc gggaagcgag aaatctcgcg cgcctgaggc accacgcggt
    37381 ctgcgagctt gttccactcc agttcgaaca ccagagtgcg accgttaaga tccagtttac
    37441 gttccagttc aggatgaaca actccaacaa aaccaatacg ttcccctttc agataaatcg
    37501 ctgcggattg ccccggatgc agtgccggat tcgcttctgc acggaactca acctcattca
    37561 gtttaccggt caggtcgaga acggattcaa gatcgccttt caaatcatag aaatcaacgg
    37621 tctcttttgc caggttccag tgctcttcgt aacggttacc gcaaatcaca ccggctaaca
    37681 tcagatcctg acgaatgccc aacggtgcct gagtatctgg tacgaaacgc agaccgcttt
    37741 cgaaaatgcg cacacggttc tgctgacggt tctggttgta caccacggtt gccagcaggc
    37801 cggtccacag agaaagacgc attgctgaca tttcaacaga gatcgggctt ggcagcagta
    37861 aggcttcaac gcctggatgg atcatctgct gcactttcgg atcaacgaag ctgtaggtga
    37921 tcacttcctg atagcctttg tcgttgagca gcgttttcac gcgcttgagc gacaggtcag
    37981 cttcacggtg agtacccata atcaggcttg cctgtaccgg ctcatccggg atgttgttgt
    38041 agccgtaaac acgcgcgact tcttcaacca gatcttcttc aatctccata tcgaaacgcc
    38101 agctcggcgc aactgcctgc cactcgtctt tgccttcggt cacttcgcag ccgagacgac
    38161 gcagaatgtc agttacctgt tcatcagcaa tatgatggcc gatcaggcga tccagtttgc
    38221 tacgacgtaa agtgatggtt gcacgcttcg gcagcgttgc ttcgttggtg atatcaatta
    38281 ccggaccagc ctcaccaccg cagatgtcga tcagcagacg ggtcgcacgt tccatcgctt
    38341 tgtgctgcag tgccggatca acgccacgct cataacggtg agacgcatca gtatgcaggc
    38401 catgacgacg agcacgaccg gtgatagaca gcgggctgaa gaaagcgcat tccagcaaga
    38461 cgttttgcgt ttcgtcattc acgccagagt gttcgccacc aaagatgccg cccatcgcca
    38521 gcgccttgtt gtggtcggcg atgaccagag tgtcagcatt cagcttcgct tcagtgccat
    38581 caagcagcac cagcgtttcg ccctcttttg ccatccgcac cacaatgccg ccttcaatgc
    38641 gatctttatc gaaagcgtgc atcggctggc ccagttcgag cagtacatag ttggtgacgt
    38701 caacaactgc atcgatagaa cggatcccgc aacgacgcag tttttctttc atccacagcg
    38761 gagttggcgc tttaacgtta atgcctttta ccacacggcc aagatagcgc gggcaggctt
    38821 caggcgcttc gactgtaatc ggcaacgtgt catcgatggt cgcaccaacc ggaacgattt
    38881 ccggttcaac cagcggcagc tgattcagca cggcaacgtc acgcgcaaca ccaatgatac
    38941 ctaagcagtc ggcacggttt ggcgttacgc tgatttcgat ggtgttgtca tcgagtttca
    39001 ggtattcgcg gatgtcagtg ccaatcggcg catctgcagg cagttcgata atgccgctgt
    39061 gatcgtcaga aattcccagt tcagagaagg agcacagcat cccttcagac ggttcgccac
    39121 gcagtttcgc cgctttaatt ttgaaatcac ccggcagaac agcaccaatg gtagctaccg
    39181 ccacacgcag gccctgacgg cagtttggcg caccacagac gatgtccagc aggcgatcgc
    39241 cgccgacatt cacttttgtc acacgcagtt tgtcagcgtt cggatgctgc gcacactcaa
    39301 ccacttcacc aacgaccacg ccgtggaagc taccggcaac cggttctaca ccgtcaactt
    39361 ccaggcccgc catagtgatt tgattcgcca gcgcatcgct atcaatcgcc gggttcaccc
    39421 attcgcgtaa ccacagttca ctgaatttca taatctattc ctgccttatt taaactgttt
    39481 gaggaaacgc agatcgtttt cgaagaatga acgcaggtcg gtgacgccgt aacgcaacat
    39541 agtcagacgc tccatcccca tcccgaaggc gaaaccagag taaacttccg ggtcgatgcc
    39601 aacgttacgc agcacgttcg gatgcaccat cccgcaaccc agcacttcca gccatttacc
    39661 gtttttaccc atgacgtcaa cttctgcaga aggctcggtg aacgggaagt aggaaggacg
    39721 gaagcgaatc tgcaaatctt cctcaaagaa gttacgcagg aaatcgtgca gagtgccttt
    39781 caggttggta aagctgatgt tggtatcaac aatcagacct tccatctgat ggaacatcgg
    39841 cgtgtgagtc tggtcgtagt cgttacgata aacgcggcca ggcgcgatga tacgaatcgg
    39901 tggctgctgg gctttcatgg tgcggatctg tacgccagag gtctgggtac gcagcaggcg
    39961 ggtagtgtca aaccagaaag tgtcgtggtc agcgcgcgcc gggtggtgac caggaatgtt
    40021 cagagcatcg aagttatgat aatcgtcttc gatttccggc ccggttgcca cggtaaagcc
    40081 aagctcaccg aagaaacttt cgatacggtc gatggtacgg gtaaccggat gcagaccgcc
    40141 gttttcaatg cgacgaccta gcagggagac atcaatcgtt tccgccgcca gacgcgcatt
    40201 cagcgctgcg ctttccagtt ccgctttacg cgcattcagc gcctgctgaa cctgctcttt
    40261 cgcttcgttg ataaccgcac cagctgccgg acgctcttct ggcggcagct cacgcagggt
    40321 cgtcatctga agggttaagt gccctttttt acccaaatat tcgacgcgca cattatctaa
    40381 cgcggcaaca tctgacgcct ggctaatggc cgccttcgca ctggcaacca gttctgcgag
    40441 atgtgacatg gttttcctca ttgtgtcagt ggtgacactg gttcgttgga cttagagcct
    40501 atcccatcag gctattttac ttgccatttt ggtcccgggc agtgctcgaa attctcacgt
    40561 acttaaatac gctccggttt ctccgcgctg gccgtgtcca gactggctgc gacaattacg
    40621 cctgatagac tggcttttta tttttcaaaa cgcgcataca aaaaaagcct ccactgggag
    40681 gctttcaggc gctgttttcc gtttctcttc tcacgcgcta gcctcctgga ttcaggtgct
    40741 aaagtaaaaa aagaagcgga aaatagcagc attcattgct tgcgttacct tttggtactc
    40801 ttcaaaagac ctttattgaa aaggctacgg cgataaaagt caatgttttg atggcgttga
    40861 aacgaaaaga gggagactag ctccctcttt caactggctt atgccagagc tgctttcgct
    40921 ttttcaacca gagcggtgaa cgctactttg tcgaatactg cgatatcagc caggatctta
    40981 cggtcgattt caacagaggc ttttttcagg ccattgatga atttgctgta agaaataccg
    41041 ttctgacgtg ctgctgcgtt gatacgcgca atccacagtt gacggaactg acgcttacgt
    41101 tgacgacggt cacggtaagc atactgacca gctttgataa cagcctggaa ggcaacgcgg
    41161 tatacgcgag aacgcgcacc gtagtagcct ttagcttgtt tcaaaatttt cttgtgacgt
    41221 gcgcgtgcaa taacaccacg ttttacgcga gccatatgtg ctctcctgta tctatattct
    41281 aattaaaaag ttaaaaacgt taacggctta tgcgtacggc aggcacgcga ttaccaggcc
    41341 cagatcgcct ttggaaacca tggctttcgg acgcaggtga cgtttacgtt tggtcgcttt
    41401 tttggtcaga atgtgacgca ggttagcgtg cttgtgctta aaaccacctt taccggtttt
    41461 tttgaagcgc ttagcagcac cgcgtacggt cttaattttt ggcattttaa taacttccac
    41521 ttcgcattgt taataaacga aacaaaggcg aacaaagcct gtgaagcccg aaggctccac
    41581 agacagtgct acttgaaggc cttactgttt cttcttagga gcgagcacca tgatcatctg
    41641 gcggccttcg atcttcgttg ggaaggattc gaccactgcc agttcttgca aatcgtcttt
    41701 cacgcgatta agcacttcca taccgatttg ctggtgcgcc atctcacgac cgcggaaacg
    41761 cagcgtgatt ttggctttat caccctcttc gagaaagcga atcaggctgc ggagttttac
    41821 ctgatagtcg ccttcatctg taccaggacg gaatttaatt tccttaacct ggataacttt
    41881 ttgctttttc ttctgttcct tagaagactt gctcttttca tagaggaatt tgccgtaatc
    41941 cattatacga caaaccggcg gctcggcgtt agggctgatc tcgactaagt ctactccggc
    42001 ttcttctgct ttctccagag cttctctcag actcacaata ccaagctgct cgccttccag
    42061 acctgttaag cgaacttcct gggcgcgaat ttcgccattg atacggttag ggcgcgccgt
    42121 ttgaactcgt tttccgcctt taatacctta ttcctccaat tgtttaagac tgcggctgcg
    42181 aatctcttgt tgcagcttct cgatcacttc atttacgtcc atgcttccca ggtctttacc
    42241 acggcgggtg cgaacggcaa ctttgcctga ttccacctct ttatcaccac agaccagcat
    42301 atatgggacg cgacgcaaag tgtgctcgcg gattttaaag ccaatcttct catttctcaa
    42361 gtctgcttta acacgaatgc ccgcatttga tagtttttgc gtcaattcgt taacgtattc
    42421 agactgtgaa tcggtaatat tcatgataac aacctgaact ggcgcaagcc aggtcgggaa
    42481 gaaaccagcg aattcttcgg tcaggatacc gatgaaacgt tccatcgacc ccagaattgc
    42541 gcggtgaatc attaccggta ccttacgctc gttgtcttcg cccacatagg aggcgctcag
    42601 acgagacggc aaggagaagt ccagctgtac tgtaccgcac tgccatgcac gatcgaggca
    42661 gtcatacagg gtaaattcaa ttttcggacc gtagaaagcg ccttcaccca gttgatattc
    42721 aaacgggatg ttgttttctt ccagcgcaac cgccaggtcc gcctcagcac gatcccacat
    42781 ttcgtcgctg ccaatacgtt tttcaggacg agtggagagt ttgacgacga tcttctcgaa
    42841 gccaaaagtg ctgtacatat catagactaa acggatacat ccgttaactt catcgcgaat
    42901 ttgttcttca gtacagaaga tatgcgcgtc atcctgggta aatccacgca cgcgcatcag
    42961 gccatgcagc gaacctgacg gctcgttacg gtggcagcta ccaaactcgg ccatacgcag
    43021 cggcagatcg cgataagact tcagcccctg gttgaaaatt tgtacgtgac ccgggcagtt
    43081 catcggctta atgcagtatt cacggttctc agaagatgtg gtgaacattg catctttgta
    43141 gttgtcccag tgaccggttt tttcccacag gacacggtcc atcatgaacg gacctttaac
    43201 ttcctgatac tggtactctt tcagtttaga acgaacaaac acttccagtt cacggaagat
    43261 ggtccagccg tcgttgtgcc agaataccat acccggcgct tcttcctgca tatggtacag
    43321 gtcgagctgt ttaccgattt tacggtggtc gcgtttcgcg gcttcttcca ggcgctgcag
    43381 gtaagcgtta agtgcttttt tgtctgccca cgccgtaccg taaatacgtt gcaacatttt
    43441 gttgttgctg tcgccacgcc agtaagcccc tgccgttttc attagtttga aatgatggca
    43501 gaaacgcatg ttcggtacgt gcggaccgcg gcacatatcg acatattctt catggaagta
    43561 cagacctggc ttgtcatcat gggcgatgtt ttcgtcaaga atggagactt tgtagctctc
    43621 cccacggttg gcgaaagttt cacgcgcttc gtgccagctg actttcttct taatgacgtc
    43681 gtagtttttc tcagcaagct catgcatccg cttctcgagt gcttcgacat cttcctgggt
    43741 taacgtgcgg tcaagatcaa cgtcgtaata aaaaccgttg tcaataaccg ggccgattgc
    43801 cattttggta tgcggccaaa gttgtttaat cgcatgccct aacaggtgcg cacaggagtg
    43861 acgaatgatc tccagacctt cttcgtcttt ggcggtaatg atcgacagtt gtgcgtcgtt
    43921 ttcaatcaga tcgcaagcat caaccagttc gccattaacg cgccctgcga tacaggcttt
    43981 cgccagacct ggaccaatgt ccagcgcaac atccatgggg cttacagcgt ggtcgtaatg
    44041 gcgttggctg ccatcaggaa gagttataac aggcatttta tatccttaat ttgcagtggt
    44101 gacccacacg aaagatcaca tacaaagaaa aatttgttta ttaacagttc attgcgaaac
    44161 catctagcca acaaatgcta aattggttcg caatcaggta cacaaatgaa agacattaac
    44221 ctctcacctt ccacccggtt gaacgatgtt aacactaaaa aaagggagat tgtacctttc
    44281 cgtttcacat tgattcgatt cgcaattcgt ttgtagtgag tacaccagca aaacgcttaa
    44341 agaaccactg ataacatcac ggtgcgtctg aaaggtttac tgcgagcgga tttacagcag
    44401 cataaactga atccagcaaa gttcgttgcg tgatttggcg gtagtatttt aaacgctcat
    44461 gagattaatg acgaagtggt catatcacaa tgataaaagt gacacaattc ttatagcaat
    44521 ttttcgtgca catttcgttc tggcgataat aattaatcat catcctcaaa acctccagac
    44581 atatataagg cgaatagatt atgagtcaaa acgatatcat tatcagaacc cattataagt
    44641 ctcctcatag attgcacatc gatagcgaca taccaacgcc ttcatcagag cctattaatc
    44701 aatttgcgcg ccaactcatc accctacttg atacctctga cttaagttcg atgctgtcat
    44761 actgtgttac tcaggaattt accgcaaact gtcgaaaaat ttcacaaaat tgttattcca
    44821 ctgccctttt tatcattaac tttgccactt cacccatcca tgcagaaaat atactcatta
    44881 cattacacta taaaaaagaa atcattccct tatttctgga aaccacgcct attaaggcta
    44941 accatttgcg aagcatactg gattatattg aacaggaaca gttaactgcc gaagatcgta
    45001 accattgtat gaaactgtct aaaaaaatcc atagagaaaa aactatacaa ccaacagtaa
    45061 atctcaatgg tagtgcattt ttttcgcaat ctccttctga cgctattttt tgtcgccatc
    45121 tgtcattgca atacgccctt gattcattga gaaatggaaa aggcaaagtc aacctgatta
    45181 aacactactc ctccgttgaa tccatacagc agcatgtccc cttagtccgg gacgcggagt
    45241 tcagagcatt acttcgccat cctcctgcag ggagtcgcgt tatcgcgagt aaggattttg
    45301 gcttcgcttt agatattttc ttttgtcgaa tgatggcaaa caatgtcagt catatgtccg
    45361 cgattttata tatagacaat catactttgt cagtaaggct acgaataaag cagtcagtgt
    45421 atggacaatt aaattatgtt gtgtctgttt acgacccgaa cgataccaac gttgccgtca
    45481 gaggcaccca cagggcagca cggggctttc tctcgcttga taagttcatc agttcagctc
    45541 ccgatgctca gacctgggct gataggtatg ttcgcaactg tgcaattgct attcttcctc
    45601 tattacctga gggagttcca gttgctattt tagcgggtat cacgacacga atgccatttg
    45661 cccctataca tccatcggca atgttgttaa taatggccac aggccagact caatagctta
    45721 ttacactatt caaacagttg cccatactcc ctgaaaaaga aatcattgaa ataataactg
    45781 cgcagaatag cgttggtaca cctgctttat ttctggctat gatgaacgga caaactgaca
    45841 acgtaaaaat atttatgcaa gaaattcagt cactggtata caatcatatc attcatgaag
    45901 ataatctggt taaattactg caaactaaaa gtgctaacga aacacctgga ctctatatct
    45961 ccatgttgta tggattcgat gaaataatcg atatctttct gaatgcatta accactccaa
    46021 tagcacaaga acttttaaac aaaaaaatgg tgatgagtat tttagccatg aaaatacatg
    46081 atggtgagcc aggattatac gccgcaatgg aaaataatca ccctttgtgt gtcacacggt
    46141 tcctctctaa aattaatggc atcgccttta aatacaagtt gagcaaagct aacatcatgg
    46201 atttattaaa aggagctaca gcacagggaa cccctgcatt atacatcgcc atgagcaagg
    46261 gtaatgaaga cgtcgtgtta tcttatatat cgacgctggg tgcttttgca aaaaaacatt
    46321 cttttagtca acatcagtta tttacactat tggccgctaa aaatcatgac aacatgtcag
    46381 ctgttcatat agccattcat cataagcatt ataaaactgt agaaacatat tatgctgcta
    46441 taaatgtcat cagccaaagc ctgagtttta gtgctgatga aataaagacg tatttataac
    46501 cagctattta cgatatatat cgatacataa ttttattttt ttcataaaac gcagaaacag
    46561 tgaccatgtg cacaacgcca cccgacggct gtaataacaa aaaatccctt gatgcctatc
    46621 ccttttgtta cactccgtta tcacgcacaa gagatatgca ggacactggt atgccgacta
    46681 aacgctttga taaaaaacac tggaagatgg tggtggtgct actggcaatc tgtggcgcta
    46741 tgttgttgct acgttgggca gcaatgattt ggggctgagc agtgacaaac gaagacagcc
    46801 agaccgcata acacggtccg gcagataatt ttacatgctg tagcccagcg ataaggttgt
    46861 ccgacgatcg gtatgttccg gtgcagactc tggaggttca gagttccatg tgacgttgta
    46921 agccaccttc agtccaaaat gttcattaat agcaacattt aatgcgcttt cggagttcag
    46981 cgttgtatct tccgcgccaa agaccgaaac accctgcgta aatttagcgt tgtcagtcaa
    47041 ctgccatgca taagccccgg aggcatagcc cagcggctgg gtttcactgg cattgtcggt
    47101 atatttgtcg taacgcacac ctgggccgaa ttcaaagcgg aaactgtgta ccgggccatt
    47161 caaaaactgg cgaccataac ccgcggtcaa cacatcgcgc tctcgatagc cgttataacg
    47221 gtctgtcagc cagcttgcct gaccaaataa atagtcataa tcagttaaat taaaacggct
    47281 acgcccgccc gccgcatatt tttctgaaga acgctcatca ttagaagaag tattactggc
    47341 gttcccccac agcgaccagg cagtggtttg tccataccag gtcatggtgg tgtcagccgt
    47401 aagtgaggag cttttcgtat tgcctgattg tgcaagatat cctgcgttca gattaccttc
    47461 gaaaggtttt ttagcgctgg caggatcgtc catgacagta aaaacggaat catcggcagc
    47521 tgcattcagt gacgcaaaca tgccccccgc caacataacg atggcaggaa ctgtcttcaa
    47581 aagcttcatt tatcaagagt ccgtacaaca aaaaaagaga ccatcgcggt cccggaaact
    47641 ttcttaagga tcaaagatta gcgtccctgg aaaggtaacg aattataaaa aggcgcgaat
    47701 aacttagcaa tgtattctta tttcattttt tgaataagca tgtggcgaaa acagattttt
    47761 atttatatat atttatctgc aaaattttaa ataaagctcc aataaatcat attgttaatt
    47821 tcttcacttt ccgctgattc ggtgccagac tgaaatcagc ctataggagg aaatgatggt
    47881 acgtatctat acgttgacac ttgcgccctc tctcgatagc gcaacaatta ccccgcaaat
    47941 ttatcccgaa ggaaaactgc gctgtaccgc accggtgttc gaacccgggg gcggcggcat
    48001 caacgtcgcc cgcgccattg cccatcttgg aggcagtgcc acagcgatct tcccggcggg
    48061 tggcgcgacc ggcgaacacc tggtttcact gttggcggat gaaaatgtcc ccgtcgctac
    48121 tgtagaagcc aaagactgga cccggcagaa tttacatgta catgtggaag caagcggtga
    48181 gcagtatcgt tttgttatgc caggcgcggc attaaatgaa gatgagtttc gccagcttga
    48241 agagcaagtt ctggaaattg aatccggggc catcctggtc ataagcggaa gcctgccgcc
    48301 aggtgtgaag ctggaaaaat taacccaact gatttccgct tcgcaaaaac aagggatccg
    48361 ctgcatcgtc gacagttctg gcgaagcgtt aagtgcagca ctggcaattg gtaacatcga
    48421 gttggttaag cctaaccaaa aagaactcag tgcgctggtg aatcgcgaac tcacccagcc
    48481 ggacgatgtc cgcaaagccg cgcaggaaat cgttaatagc ggcaaggcca aacgggttgt
    48541 cgtttccctt ggtccacaag gagcgctggg tgttgatagt gaaaactgca ttcaggtggt
    48601 gccaccaccg gtgaaaagcc agagtaccgt tggcgctggt gacagcatgg tcggcgcgat
    48661 gacactgaaa ctggcagaaa atgcctctct tgaagagatg gtccgttttg gcgtagctgc
    48721 ggggagtgca gccacactca atcagggaac acgtctgtgc tcccatgacg atacgcaaaa
    48781 aatttacgct tacctttccc gctaacaaaa acattccccc agcattgggg gaatcaccac
    48841 caacctgtcg gcaacgcgtt tctccgactt tgctcaaaag tcatgtgata acaaaggggt
    48901 gaactatggc cagtggcgat cttgtccgtt atgtcataac cgtcatgttg catgaggata
    48961 cattgactga aattaacgag ttgaataatt acctgactcg cgacggtttt ttgctcacca
    49021 tgacggatga tgagggaaat atccatgagc tggggactaa cacttttgga cttatcagta
    49081 cccaaagtga agaagaaatt agagaactgg tttcggggct tacccaaagt gcaaccggca
    49141 aagatcctga aatcaccatc acgacttggg aggaatggaa tagcaacaga aaataaatgg
    49201 tttttgggca ataatcagtc tgtggtgtgc gttagctcgt gtttttacac cgcattcttg
    49261 cgctaacctt atgatctggc agacaacatg ggagagacat catgtggcag gcaatcagtc
    49321 gtcttttgag cgagcagtta ggtgaaggcg aaatcgaact gcgtaatgaa ctgcctggcg
    49381 gagaagtcca tgccgcatgg catttgcgct atgcaggaca tgactttttc gtcaaatgtg
    49441 atgaaaggga actgcttccc ggttttaccg ccgaagccga ccaactggag ttactgtcgc
    49501 gtagtaaaac cgtcaccgtg cctaaggttt gggcagtagg cgctgaccgt gactacagtt
    49561 ttctggtgat ggattatctc ccacctcgtc cgctggatgc gcatagcgca tttattcttg
    49621 gtcagcaaat tgcgcgttta catcaatgga gtgaccaacc acaatttggc ctcgattttg
    49681 ataactcgct ctccacaact ccacagccca acacctggca acgtcgctgg tcaacgtttt
    49741 ttgctgaaca acggattggc tggcagttgg aactggcagc agagaaaggg atcgctttcg
    49801 gcaatatcga cgccatcgtc gagcatattc agcagcgtct ggcctcgcat caactgcagc
    49861 cttctctgtt gcacggcgat ttatggtccg gcaactgtgc gctgggtccg gatggcccgt
    49921 acattttcga ccctgcctgc tactggggtg accgagagtg cgacctagcg atgttaccgc
    49981 tgcatactga acagccgcca caaatctatg acggctatca gtcagtatcc ccgctacctg
    50041 ccgatttcct tgaacgtcaa ccggtttacc aactctacac gctgttaaat cgtgcaagat
    50101 tatttggcgg tcagcatttg gttattgctc agcagtcatt ggatagatta ttagcagcat
    50161 gatatgggtt gaggataatg gccgctccgt gcggcctttt gattaaataa acccaagcag
    50221 agaaaagaac acataacctg cagcaataca aataaccggc aggatatata gtgagaaaaa
    50281 ctgcaggaaa atagtatggt gcggaacaac aatacgggac tcaatttgtt cacgcgttag
    50341 cccctcttcc cctttggctt tttccagaat gagttgatct tcaacacctt cccgcaagaa
    50401 gcgcgcctgg cgactcatcc gggcaccgga atcctgcaac gccagtccga caaaaatcag
    50461 gatgaaaatc acccagaaca taacgttcac gccaccatta aaattagggg tcggggagtt
    50521 ataccagaac agattcaaaa aaggcgtatt cgcctgcatc atatcgatca tgacatgagt
    50581 aaagtcgagc atgaccgcat taatgccttc ctggttttcc tgccgggtat tcataaattt
    50641 cagcaaagaa atcaatgtgg agatcaatgc aggtataaaa atcacccaac ccaaaatcct
    50701 tttcaaaaca gcaatgcgtc cagcttgttg atacgtcatg agttctcctt gattaagacg
    50761 cgtcgtttca cttagtttac ctgtagatat ctgttttcgc ccattcttta aaggcgatat
    50821 gataggcgct taatcataag cacggcttaa taccttacac ataatgctct aaaggagagg
    50881 ttgtaatgtc aaccccgcgt cagattcttg ctgcaatttt tgatatggat ggattactta
    50941 tcgactcaga acctttatgg gatcgagccg aactggatgt gatggcaagc ctgggggtgg
    51001 atatctcccg tcgtaacgag ctgccggaca ccttaggttt acgcatcgat atggtggtcg
    51061 atctttggta cgcccggcaa ccgtggaatg ggccaagccg tcaggaagta gtagaacggg
    51121 ttattgcccg tgccatttca ctggttgaag agacacgtcc attattacca ggcgtgcgcg
    51181 aagctgttgc gttatgcaaa gaacaaggtt tattggtggg actggcctcc gcgtcaccac
    51241 tacatatgct ggaaaaagtg ttgaccatgt ttgacttacg cgacagtttc gatgccctcg
    51301 cctcggctga aaaactgcct tacagcaagc cgcatccgca agtatatctc gactgcgcag
    51361 caaaactggg cgttgaccca ctgacctgcg tggcgctgga agactcggta aatggcatga
    51421 tcgcttctaa agcagcccgc atgcgttcca tcgtcgttcc tgcgccagaa gcgcaaaatg
    51481 atccacgttt tgtattagca aacgtcaaac tctcatcgct gacagaactc accgcaaaag
    51541 accttctcgg ttaatgacca ggggcagtga tcacgctgcc cctggttctt tatctgaatt
    51601 tgcattcaac tgacggatta atcgtcaatt taagagaaag agttacaccg tcaccacttc
    51661 cgtgcactgt ataaaaatcc tatactgtac gtatcgacag tttagtgagt tttatcatga
    51721 cggcggaagg tcaccttctc ttttctattg cttgtgcggt atttgccaaa aatgccgagc
    51781 tgacgcccgt gctggcacag ggtgactggt ggcatattgt cccttccgca atcctgacgt
    51841 gtttgttacc ggacatcgat cacccaaagt cgtttcttgg gcagcgatta aaatggatat
    51901 caaaaccgat cgcccgcgct tttgggcatc gtggttttac ccacagtctg ctggcggtat
    51961 ttgcgctgct ggcaaccttt taccttaagg ttccggaagg ctggttcatt ccggctgatg
    52021 cgctacaagg aatggtgctg ggttatttga gccacatact tgccgatatg ctgacacccg
    52081 ccggtgttcc cctgctctgg ccatgccgct ggcgtttccg cttgcctatc ctggttcccc
    52141 aaaagggcaa ccaactggaa cgttttatct gcatggcatt atttgtctgg tcggtatgga
    52201 tgccccattc attacccgag aacagcgctg ttcgttggtc atcgcaaatg atcaatacct
    52261 tgcagatcca gtttcatcgg cttattaagc atcaggttga atactaaaaa ggcaaaaatc
    52321 acctttctgg aataagcaat tccatttgaa tataagagcc agctcacagt tctgttaatc
    52381 ttgcgccaac actatgactg ctacgcagtg atagaaataa taagatcagg agaacgggga
    52441 tgaactttcc attaattgcg aacatcgtgg tgttcgttgt actgctgttt gcgctggctc
    52501 agacccgcca taaacagtgg agtctggcga aaaaagtgct ggtgggtctg gtgatgggtg
    52561 tggtttttgg ccttgccctg cataccattt atggttctga cagccaggta cttaaagatt
    52621 ctgtacagtg gtttaacatc gttggtaacg gctatgttca actgctgcaa atgatcgtta
    52681 tgccgttagt cttcgcctct attctgagcg cggttgcccg tctgcataac gcatctcagt
    52741 taggcaaaat cagttttctg accatcggta cgcttttgtt taccacgctg attgcggcgc
    52801 tggtcggtgt gctggtcacc aacctgtttg gtttgacggc tgaaggtctg gttcagggtg
    52861 gtgcagaaac tgcacgtctg aacgccattg aaagtaacta tgttggtaaa gtctctgatc
    52921 tgagcgttcc gcagctggtc ttgtccttta tcccgaaaaa cccgtttgcc gatcttaccg
    52981 gagccaatcc gacgtcaatt atcagcgtgg taatttttgc cgcattcctc ggcgtagctg
    53041 cattgaaact gctgaaggat gatgcgccga aaggtgaacg cgtcttagcc gctatcgata
    53101 ccctacaaag ctgggtgatg aaactggttc gccttgtcat gcagttgacc ccttacggcg
    53161 ttctggctct aatgaccaaa gtggttgcag gttctaactt gcaagacatc atcaaactgg
    53221 gaagtttcgt tgtcgcgtcc tacctcggtc tgctgattat gtttgcagtg catggcattc
    53281 tgctgggcat taatggcgtg agtccgctga agtacttccg taaggtatgg cctgtgctga
    53341 cgtttgcctt taccagccgt tccagtgctg cgtctatccc actgaatgtg gaagcacaaa
    53401 cgcgtcgtct gggcgttcct gaatccatcg ccagtttcgc cgcctctttc ggtgcaacca
    53461 ttggtcagaa cggctgcgcc ggtttgtatc cggcaatgct ggcggtgatg gttgcgccta
    53521 cggttggcat taacccgctg gacccgatgt ggattgcgac gctggtcggt attgttaccg
    53581 ttagttccgc aggcgttgcc ggtgtcggtg gtggtgcaac tttcgccgca ctgattgtgc
    53641 tgcctgcgat gggcctgcca gtaaccctgg tggcgctgtt aatctccgtt gaaccgctta
    53701 tcgacatggg ccgtacggcg ctaaacgtta gtggctcgat gacagctggc acgctgacca
    53761 gccagtggct gaagcaaacc gataaagcca ttctggatag cgaagacgac gccgaactgg
    53821 cacaccgtta attcttatgc tggcaacggt ccgttttgta taggggccgt tgctttactt
    53881 tttaaatgtt ccatcgcggc gatgcatact gactcatatt taaacagatc gtcagcgaaa
    53941 aaaatgtcca acttatcaat acattcctgg ctcaatgaat gtgcctgtag tgagaagctt
    54001 aaaacctcat acccgagacg ttttctaagt gaactatctg gaatactcat aatatcttca
    54061 agcacaccac ttcccattcc cggaagattt agtggattaa aaagaagatt gatattaagc
    54121 attgatatta tcgaaggaaa ctttaggaga ctctcatttt ttttgaatcg cagcaaaaat
    54181 gctcccggca ggatgtatat aatttttttc atagtatata gtccttaaat aatgaataac
    54241 caacctattt tttatattga tcaaattcgt cattttttcg ggtcggaatt cacaggtaaa
    54301 atccatccca acaaaattct cattaatatc tcttatccga aactcactgc cataaagatt
    54361 attaattatc actctatatt taacattgtc ctgcagatac gtatcaaaac taagtgctgg
    54421 agctcctggc tttgaggcat catagttaat gttagacata taggcaatca tacgattgaa
    54481 gttcgactct gctatttcat cgcagtcaca ttgacccgtt gatataaaga aagtgagtgc
    54541 cgccatggta gactcaagcc tttctttaaa agagtcgaaa gccaggcttt taatatttaa
    54601 atcaccataa ttactctgta ttaagtttgt agaaaacatc tcccgcccca tattgttaac
    54661 aaaattatta tctcatttaa atctaagtca cttacaatat aagtttaaga gcgacgccac
    54721 aggatgaact atcaaaaata gctcatcatg attagcaaaa cttaaccatt ttaaaataaa
    54781 taaacaatta aagaaaaaag atcacttatt tatagcaata gctcgtcaaa ggcagctttt
    54841 tgttacaggt ggtttgaatg aatgtagcaa cgaaatgcag aatttcaggt catgtaactc
    54901 ccggcaaaac cgggaggtat gtaatcctta ctcagtcact tccccttcct ggcggatctg
    54961 atttgcccaa cgttgggcag attcaggcac agtaaacgcc ggtgagcgca gaaatgactc
    55021 tcccatcagt acaaacgcaa catatttgcc acgcagcatc cagacatcac gaaacgaatc
    55081 catctttatc gcatgttctg gcggcgcggg ttccgtgcgt gggacatagc taataatctg
    55141 gcggttttgc tggcggagcg gtttcttcat tactggcttc actaaacgca tattaaaaat
    55201 tagaaaaact gtagtttagc cgatttagcc cctgtacgtc ccgctttgcg tgtatttcat
    55261 aacaccgttt ccagaatagt ctccgaagcg ggatctggct ggtggtctat agttagagag
    55321 ttttttgacc aaaacagcgg ccctttcagt aataaattaa ggagacgagt tcaatgtcgc
    55381 aacataacga aaagaaccta catcagcacc agtcaccact acacgattcc agcgaagcga
    55441 aaccggggat ggactcactg gcacctgagg acggatctca tcgtccagcg gctgaaccaa
    55501 caccgccagg tgcacaacct accgcccctg ggagcctgaa agcccctgat acacgtaacg
    55561 aaaaacttaa ttctctggaa gacgtacgca aaggcagtga aaattatgcg ctgaccacta
    55621 atcagggcgt gctcatcgcc gatgatcaaa actcactgcg tgccggtaac cgtggtccaa
    55681 cgctgctgga agattttatt ctgcgcgaga aaatcaccca ctttgaccat gagcgcatcc
    55741 cggaacgtat tgttcatgca cgcggatcag cagctcacgg ttatttccag ccatataaaa
    55801 gcttaagcga tatcaccaaa gcggatttcc tctcagatcc gaacaaaatc accccagtat
    55861 ttgttaggtt ctctaccgtt cagggtggcg ctggctctgc agataccgtg cgtgatatcc
    55921 gtggttttgc caccaagttc tatactgaag agggtatttt tgacctcgtt ggcaataaca
    55981 cgccaatctt ctttatccag gatgcgcata aattccccga ttttgttcat gcggtaaaac
    56041 cagaaccgca ctgggcaatt ccacaagggc aaagtgccca cgatactttc tgggattatg
    56101 tttctctgca acctgaaact ctgcacaacg tgatgtgggc gatgtcggat cgcggcatcc
    56161 cccgcagtta ccgcaccatg gaaggcttcg gtattcacac cttccgcctg attaatgccg
    56221 aagggaaggc aacgtttgta cgtttccact ggaaaccact ggcaggtaaa gcctcactcg
    56281 tttgggatga agcacaaaaa ctcaccggac gtgacccgga cttccaccgc cgcgagttgt
    56341 gggaagccat tgaagcaggc gattttccgg aatacgaact gggcttccag ttgattcctg
    56401 aagaagatga attcaagttc gacttcgatc ttctcgatcc aaccaaactg atcccggaag
    56461 aactggtgcc cgttcagcgt gtcggcaaaa tggtgctcaa tcgcaatccg gataacttct
    56521 ttgccgaaaa cgaacaagca gcattccatc caggtcatat tgttcctggt ctggacttca
    56581 ccaacgatcc gctgttgcag ggacgtttgt tctcctatac cgatacacaa atcagtcgtc
    56641 ttggtgggcc gaatttccat gagattccga ttaaccgtcc gacctgccct taccacaact
    56701 tccagcgtga tggcatgcat cgtatgggga tcgacactaa cccggcgaat tacgaaccga
    56761 actcgatcaa cgataactgg ccgcgcgaaa caccgccggg accgaaacgc ggcggttttg
    56821 aatcatatca ggaacgcgtg gaaggaaata aagttcgtga gcgcagtcca tcgtttggcg
    56881 aatattattc ccatccgcgt ctgttctggc taagtcagac gccattcgag cagcgccata
    56941 ttgtcgatgg tttcagtttt gagttaagca aagtcgttcg tccgtatatt cgtgagcgcg
    57001 ttgttgacca gctggcgcat attgatctca ctctggccca ggcggtggcg aaaaatctcg
    57061 gtatcgaact gactgacgac cagctgaata tcaccccacc tccggacgtc aacggtctga
    57121 aaaaggatcc atccttaagt ttgtacgcca ttcctgacgg tgatgtgaaa ggtcgcgtgg
    57181 tagcgatttt acttaatgat gaagtgagat cggcagacct tctggccatt ctcaaggccc
    57241 tgaaggccaa aggcgttcat gccaaactgc tctactcccg aatgggtgaa gtgactgcgg
    57301 atgacggtac ggtgttgcct atagccgcta cctttgccgg tgcaccttcg ctgacggtcg
    57361 atgcggtcat tgtcccttgc ggcaatatcg cggatatcgc tgacaacggc gatgccaact
    57421 actacctgat ggaagcctac aaacacctta aaccgattgc gctggcggga gacgcgcgca
    57481 agtttaaagc aacaatcaag gtcgctgacc agggtgaaga agggattgtg gaagctgaca
    57541 gcgctgacgg tagttttatg gatgaactgc taacgctgat ggcagcacac cgcgtgtggt
    57601 cacgcattcc taagattgac aaaattcctg cctgatggga ggcggcgcaa ttgcgccgcc
    57661 tcaaatgatt tacatagtgc gctttgttta tgccggatgc ggcgtgaacg ccttatccgg
    57721 cctacaaaac tgtgcaaatt caatatattg caggaaacac gtaggcctga taagcgaagc
    57781 gcatcaggca gttttgcgtt tgtcagcagt ctcaagcggc gcagttacgc cgcctttgta
    57841 ggaattaatc gccggatgca aggttcacgc cgcatctggc aaacatcctc acttacacat
    57901 cccgataact ccccaaccga taaccacgct gagcgatagc acctttcaac gacgctgatg
    57961 tcaacacatc cagctccgtt aagcgtggga aacagtaagc actctgacgg atagtattat
    58021 cgataaacgc cggatgacac atcacctcca gcgaacgatc accccgatgg cttgcgtcat
    58081 ccagcacttg caggaacagt gcctcgctaa tctcttcacc ataaaacgcg ctactgaatc
    58141 cctgagaact gcgcagatta gccggtaaat caccagagtt agatagcggc tgacggtcga
    58201 tgcgcaacgc aatcccctct tcagccgcaa acctggcaac aatcgggaaa atctgcggga
    58261 acatatgcac atgatgatgg ctatcaagat gcgtaggttt gcgtccaaat agctcaatga
    58321 aacgcaaata ctgactggca agctcctgag taatttcttc cagcggtaaa gcatcttctt
    58381 ctgccaactg ccagatccat tttcccagca caccatcgcg ggttaacccc ggcatagctg
    58441 tcagtggctt gcccatagta aggacaaagt acatccctat ggccagactc ggttcatcac
    58501 gactcaactg caccgcatgg tcaatagcct gcccattcac cagcgccgtc gtcgatgtga
    58561 caatcccatt gcgacaggcc tcgataatgc cgtagttctg acctttgctt aagccaaaat
    58621 catcggcatt aacaatcagt aagcgttcca taatcagcct cggttaatgt gcttttttaa
    58681 gctctgcgat gcagtcggca aaatttggca gccatttctc gtgcgccaga atcatctcgc
    58741 gtgccagcag ctcagcatcg cgatcggaat gcaccaacgg actaaggttt agcgccagta
    58801 acacatcgtt aaattctccg ctaagtgcgg cgttgctggc agcaatctcg aagcctttaa
    58861 tggtgtgaat cagccccatc actttatcat cgaaatgcgt aatgcgcgga tgtggcgtcg
    58921 cgccatcgcg ccccagctta caggtcattt ctaccgccca gtctgccgga atattatcaa
    58981 tatgcccatg atgcgggata ttaacgtaat gttcagcttg cttgtcgttg tagatagcgt
    59041 tgatcacttc gcacgctgca tcagagtaat aagccccacc gcgctgttcc agttctttcg
    59101 gcttaacttt caactccggg tttttataca gctcaaaaag ttgtttctcg actttctgta
    59161 ctacctgcgc tcgtgcgccg cctttgtagt attcgcccat ttcaatagcc agcatctctt
    59221 tttgcttgaa gtaataaagc aaataagaac acggcaacag attcagagaa cgaattaagc
    59281 cttcgctaaa tggcagatcg aaaatatttt taacgccaga tgcttttaac tgcccggatg
    59341 ccacaccatc aagcaattcg gcaaagcgcg acttgccatt taccagcaca tccttaatga
    59401 acaccatatg gttgaggccg aacaaatcga tagataaatc atcgctgtct ttcagcatca
    59461 gaacatcgtg aataaacatc ttcatgccga tcggaatatt acacacgccg ataaagcgtt
    59521 taaatccggt atgacgataa acggcttcag taaccattcc cgccgggtta gtgaagttaa
    59581 tcacccatgc attcggacaa agttcttcga catcttttac gatgtcaaaa atcaccggaa
    59641 tggtacgcag acctttaaac agaccgcccg cgccgttggt ttcctggcca agataaccat
    59701 gacttaatgg aatacgttca tccagttcgc acgccggtaa ttggcctacg cgcagttggg
    59761 tagtaacgaa atcagcatct ttcaatgctt cgcggcgatc cagcgtttta taaagcttca
    59821 tcgggacgcc agcgttatca atcatccgtt ggcagagttc aaaaatgata tccagtttct
    59881 ctttaccatc ttcgacatcc accagccata attcgctgac cggcaattcg tgataacgct
    59941 taataaatcc ttccagtaac tccggggtat agctgctccc gccaccaata gtgacgactt
    60001 ttaatttctg gctcataatt tctcccttca gtacagaata ctgatatctg gcatatctgc
    60061 ccccccggac ataaataatc cagcaacagg acagatatgt gaattgtcag gtataacgac
    60121 ttactgatta aattcagtca atttcttacg atagctctta ggcgtaaagg acgttaattt
    60181 cttaaacgtt ttaataaaca aactcgggct actataaccg gcctcaaacg caatatccgt
    60241 cacggaatag ttggtcattt ccagttgttt tttggcaaaa ttaatacgga tctcattaat
    60301 aatctgcatt ggtgttttgc cataatatcg ttgagtcgct cgcgtcaaat attcctgtga
    60361 tttggctgac aacgtcacca tattctccag cgccgattca ctaaactgct ctttatcatg
    60421 catcttttct accgtacttt tcagccactg cggtacatca tcaatcacct gttcttcgcg
    60481 gtaatgacgt aaacggttaa tgacataaaa agtaaccatc tcaacaaact cttccagccc
    60541 tgtttcgcgg aaattcaatg aagaaatcac tgtttccaca taggtgagaa aagcgttatt
    60601 ggtccggtat acctgcgaag cgacaaagca ataaggcaac aacggcaggt aatgctgctc
    60661 aaaaaagcgt ttactgatcc caacgttcaa tatacgcgtg gcaccaaact cataaaaact
    60721 ttgatggtgc gaacctaacg gaataaaaac aaaatcg
//
LOCUS       DAKISA010000011        59891 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.011, whole genome shotgun sequence.
ACCESSION   DAKISA010000011 DAKISA010000000
VERSION     DAKISA010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59891
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.011"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..262)
                     /locus_tag="OVW45_000731"
     CDS             complement(<1..262)
                     /locus_tag="OVW45_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCV5525252.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAG"
     gene            369..665
                     /locus_tag="OVW45_000732"
     CDS             369..665
                     /locus_tag="OVW45_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCV5525253.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(799..1224)
                     /gene="arsC"
                     /locus_tag="OVW45_000733"
     CDS             complement(799..1224)
                     /gene="arsC"
                     /locus_tag="OVW45_000733"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCV5525254.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1237..2526)
                     /gene="arsB"
                     /locus_tag="OVW45_000734"
     CDS             complement(1237..2526)
                     /gene="arsB"
                     /locus_tag="OVW45_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCV5525255.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(2580..2933)
                     /gene="arsR"
                     /locus_tag="OVW45_000735"
     CDS             complement(2580..2933)
                     /gene="arsR"
                     /locus_tag="OVW45_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCV5525256.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            complement(3472..3645)
                     /locus_tag="OVW45_000736"
     CDS             complement(3472..3645)
                     /locus_tag="OVW45_000736"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525257.1"
                     /translation="MHPLTHPLPVTAHVSLLDDDYLTPARASVNGTTRTSDQDFESVY
                     AHYQSENASELTG"
     gene            3673..3756
                     /locus_tag="OVW45_000737"
     CDS             3673..3756
                     /locus_tag="OVW45_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCV5525258.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3810..5162)
                     /gene="gorA"
                     /locus_tag="OVW45_000738"
     CDS             complement(3810..5162)
                     /gene="gorA"
                     /locus_tag="OVW45_000738"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCV5525259.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5234..6076)
                     /gene="rlmJ"
                     /locus_tag="OVW45_000739"
     CDS             complement(5234..6076)
                     /gene="rlmJ"
                     /locus_tag="OVW45_000739"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCV5525260.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6279..8321
                     /gene="prlC"
                     /locus_tag="OVW45_000740"
     CDS             6279..8321
                     /gene="prlC"
                     /locus_tag="OVW45_000740"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCV5525261.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVARVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKEVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8329..9081
                     /gene="rsmJ"
                     /locus_tag="OVW45_000741"
     CDS             8329..9081
                     /gene="rsmJ"
                     /locus_tag="OVW45_000741"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCV5525262.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(9130..10599)
                     /gene="dtpB"
                     /locus_tag="OVW45_000742"
     CDS             complement(9130..10599)
                     /gene="dtpB"
                     /locus_tag="OVW45_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HCV5525263.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRYGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(10917..11351)
                     /gene="uspA"
                     /locus_tag="OVW45_000743"
     CDS             complement(10917..11351)
                     /gene="uspA"
                     /locus_tag="OVW45_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCV5525264.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSANAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11742..12077
                     /gene="uspB"
                     /locus_tag="OVW45_000744"
     CDS             11742..12077
                     /gene="uspB"
                     /locus_tag="OVW45_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCV5525265.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12321..13820)
                     /gene="pitA"
                     /locus_tag="OVW45_000745"
     CDS             complement(12321..13820)
                     /gene="pitA"
                     /locus_tag="OVW45_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCV5525266.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            14052..15254
                     /locus_tag="OVW45_000746"
     CDS             14052..15254
                     /locus_tag="OVW45_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCV5525267.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAEDETGFTLDLNGMTVGCEKLVMATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPNVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(15570..16622)
                     /locus_tag="OVW45_000747"
     CDS             complement(15570..16622)
                     /locus_tag="OVW45_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HCV5525268.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            17005..18612
                     /locus_tag="OVW45_000748"
     CDS             17005..18612
                     /locus_tag="OVW45_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCV5525269.1"
                     /translation="MKVFHNIFKHFSSNHQDKHSDKVNSHQHHGKVDKKYRSNTVEVN
                     MLNDFSQIDNDLGLNIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKSDITYKVNGLS
                     IIPENFFTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNAN
                     TAYFGDTDGRVGAVLYALLVSGHIGIREKGWSLLCELLKHENMASSAYIHKNNKLLYN
                     LLNTRDMILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMDG
                     NKDSRINKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCHYDSETKVL
                     TSHHGIIRNEENECYCLGALQVPFNQMKDPIDPEELANIFNKKHKQHMDDRLIHLIRS
                     NAVASTPVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKYGHYGLGVDQHQKIDNYTM
                     GLNSWKIAPNERGDKKGVPGLSCFQPQ"
     gene            18874..20496
                     /locus_tag="OVW45_000749"
     CDS             18874..20496
                     /locus_tag="OVW45_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCV5525270.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETMIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNIIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDKFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYSGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            20862..21929
                     /locus_tag="OVW45_000750"
     CDS             20862..21929
                     /locus_tag="OVW45_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCV5525271.1"
                     /translation="MDKSKRHLAWWVVGLLVVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            21926..24661
                     /gene="rbbA"
                     /locus_tag="OVW45_000751"
     CDS             21926..24661
                     /gene="rbbA"
                     /locus_tag="OVW45_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCV5525272.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPRHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRSQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEISARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            24661..25785
                     /locus_tag="OVW45_000752"
     CDS             24661..25785
                     /locus_tag="OVW45_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCV5525273.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMAKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            25858..26131
                     /locus_tag="OVW45_000753"
                     /pseudo
     CDS             25858..26131
                     /locus_tag="OVW45_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            26128..26487
                     /locus_tag="OVW45_000754"
     CDS             26128..26487
                     /locus_tag="OVW45_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCV5525274.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(26607..27008)
                     /gene="nikR"
                     /locus_tag="OVW45_000755"
     CDS             complement(26607..27008)
                     /gene="nikR"
                     /locus_tag="OVW45_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCV5525275.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(27014..27820)
                     /gene="nikE"
                     /locus_tag="OVW45_000756"
     CDS             complement(27014..27820)
                     /gene="nikE"
                     /locus_tag="OVW45_000756"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCV5525276.1"
                     /translation="MTLLNVSGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(27817..28581)
                     /gene="nikD"
                     /locus_tag="OVW45_000757"
     CDS             complement(27817..28581)
                     /gene="nikD"
                     /locus_tag="OVW45_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCV5525277.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKAVYPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(28581..29414)
                     /gene="nikC"
                     /locus_tag="OVW45_000758"
     CDS             complement(28581..29414)
                     /gene="nikC"
                     /locus_tag="OVW45_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCV5525278.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(29411..30355)
                     /gene="nikB"
                     /locus_tag="OVW45_000759"
     CDS             complement(29411..30355)
                     /gene="nikB"
                     /locus_tag="OVW45_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCV5525279.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(30355..31929)
                     /gene="nikA"
                     /locus_tag="OVW45_000760"
     CDS             complement(30355..31929)
                     /gene="nikA"
                     /locus_tag="OVW45_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCV5525280.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(32040..32627)
                     /gene="acpT"
                     /locus_tag="OVW45_000761"
     CDS             complement(32040..32627)
                     /gene="acpT"
                     /locus_tag="OVW45_000761"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCV5525281.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(32682..33731)
                     /locus_tag="OVW45_000762"
     CDS             complement(32682..33731)
                     /locus_tag="OVW45_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCV5525282.1"
                     /translation="MEIPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVSALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            33863..35080
                     /locus_tag="OVW45_000763"
     CDS             33863..35080
                     /locus_tag="OVW45_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCV5525283.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVNY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKVGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(35084..35641)
                     /gene="dcrB"
                     /locus_tag="OVW45_000764"
     CDS             complement(35084..35641)
                     /gene="dcrB"
                     /locus_tag="OVW45_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCV5525284.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(35714..36379)
                     /locus_tag="OVW45_000765"
     CDS             complement(35714..36379)
                     /locus_tag="OVW45_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCV5525285.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNILLKRLADKSEINAL
                     QAS"
     gene            36600..36845
                     /gene="tusA"
                     /locus_tag="OVW45_000766"
     CDS             36600..36845
                     /gene="tusA"
                     /locus_tag="OVW45_000766"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCV5525286.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(36947..39145)
                     /gene="zntA"
                     /locus_tag="OVW45_000767"
     CDS             complement(36947..39145)
                     /gene="zntA"
                     /locus_tag="OVW45_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCV5525287.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(39219..39845)
                     /locus_tag="OVW45_000768"
     CDS             complement(39219..39845)
                     /locus_tag="OVW45_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCV5525288.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            39986..40345
                     /locus_tag="OVW45_000769"
     CDS             39986..40345
                     /locus_tag="OVW45_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCV5525289.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(40348..40617)
                     /locus_tag="OVW45_000770"
     CDS             complement(40348..40617)
                     /locus_tag="OVW45_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCV5525290.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(40607..41203)
                     /gene="rsmD"
                     /locus_tag="OVW45_000771"
     CDS             complement(40607..41203)
                     /gene="rsmD"
                     /locus_tag="OVW45_000771"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCV5525291.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            41353..42849
                     /gene="ftsY"
                     /locus_tag="OVW45_000772"
     CDS             41353..42849
                     /gene="ftsY"
                     /locus_tag="OVW45_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCV5525292.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVKAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            42852..43520
                     /gene="ftsE"
                     /locus_tag="OVW45_000773"
     CDS             42852..43520
                     /gene="ftsE"
                     /locus_tag="OVW45_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCV5525293.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSHRMLTLSDGHLHGG
                     VGHE"
     gene            43513..44571
                     /gene="ftsX"
                     /locus_tag="OVW45_000774"
     CDS             43513..44571
                     /gene="ftsX"
                     /locus_tag="OVW45_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCV5525294.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            44816..45670
                     /gene="rpoH"
                     /locus_tag="OVW45_000775"
     CDS             44816..45670
                     /gene="rpoH"
                     /locus_tag="OVW45_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCV5525295.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            45941..47044
                     /gene="livJ"
                     /locus_tag="OVW45_000776"
     CDS             45941..47044
                     /gene="livJ"
                     /locus_tag="OVW45_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCV5525296.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(47243..47626)
                     /gene="panM"
                     /locus_tag="OVW45_000777"
     CDS             complement(47243..47626)
                     /gene="panM"
                     /locus_tag="OVW45_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCV5525297.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            48050..49159
                     /gene="livK"
                     /locus_tag="OVW45_000778"
     CDS             48050..49159
                     /gene="livK"
                     /locus_tag="OVW45_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCV5525298.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            49207..50133
                     /gene="livH"
                     /locus_tag="OVW45_000779"
     CDS             49207..50133
                     /gene="livH"
                     /locus_tag="OVW45_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCV5525299.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            50130..51407
                     /gene="livM"
                     /locus_tag="OVW45_000780"
     CDS             50130..51407
                     /gene="livM"
                     /locus_tag="OVW45_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCV5525300.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVS
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            51404..52171
                     /gene="livG"
                     /locus_tag="OVW45_000781"
     CDS             51404..52171
                     /gene="livG"
                     /locus_tag="OVW45_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCV5525301.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRNQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            52173..52886
                     /gene="livF"
                     /locus_tag="OVW45_000782"
     CDS             52173..52886
                     /gene="livF"
                     /locus_tag="OVW45_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCV5525302.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            53170..54234
                     /gene="ugpB"
                     /locus_tag="OVW45_000783"
                     /pseudo
     CDS             53170..54234
                     /gene="ugpB"
                     /locus_tag="OVW45_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
     gene            54332..55219
                     /gene="ugpA"
                     /locus_tag="OVW45_000784"
     CDS             54332..55219
                     /gene="ugpA"
                     /locus_tag="OVW45_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCV5525303.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSRQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALHSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            55216..56061
                     /gene="ugpE"
                     /locus_tag="OVW45_000785"
     CDS             55216..56061
                     /gene="ugpE"
                     /locus_tag="OVW45_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCV5525304.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            56063..57133
                     /gene="ugpC"
                     /locus_tag="OVW45_000786"
     CDS             56063..57133
                     /gene="ugpC"
                     /locus_tag="OVW45_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HCV5525305.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            57130..57873
                     /gene="ugpQ"
                     /locus_tag="OVW45_000787"
     CDS             57130..57873
                     /gene="ugpQ"
                     /locus_tag="OVW45_000787"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCV5525306.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(57860..58300)
                     /locus_tag="OVW45_000788"
     CDS             complement(57860..58300)
                     /locus_tag="OVW45_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCV5525307.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            58420..>59891
                     /gene="ggt"
                     /locus_tag="OVW45_000789"
     CDS             58420..>59891
                     /gene="ggt"
                     /locus_tag="OVW45_000789"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCV5525308.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNIYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTV"
BASE COUNT        14614 a        15126 c        16148 g        14003 t
ORIGIN      
        1 acccggcttt cgctgacaac aatccacgta acatgggtat tgtcgagcaa ctacagaacg
       61 gggtaacggc tcccaacaga gcagccagga gatagcctct cccttttctg gcccccatca
      121 tctgctggat tttatggtct ggcacctttt gtcttatcag gctgacaccg gcactaatca
      181 ctataaacag caacgaaagc tcgacggcga gaaacacaaa catctctgcg gcatcttgca
      241 gcatcgcaag ccaactactc atacgtaacc tcattggata aatctatatt tccagaataa
      301 tcgaaatgaa attcagatca agatatttct ggtattatcg aaatatcaac cagacagagg
      361 ggtgtcatat gcagttggaa gaagtcgcta aagcgctgaa agagctaggt cacccgaccc
      421 gtttgttcat atttaaacat cttgtcaaag ccggggaaca agggttgccg gtcggtgagt
      481 tgcaaaagca gcttgggata cctggctcca cactcagcca ccatatatca gcactggtat
      541 cggtaggact ggtcaaacaa aaccgtgaga gtcggacgct gatgtgtgtt tcgcaatacg
      601 caatgctgga agcgataatt gaattcttac gcgaggagtg ctgcgtaaat agcaaaattg
      661 cctgatgcgc tacgctctac atatttcatg ccatatattg aatttgcacg gttttgtagg
      721 tcggataaga cgcgttagcg tcgcatccga cataaacaaa gcgcacttta ccaactatcg
      781 gcagtggata tcgccgcttt atttcaggcg cttacccgct tcatcaacca ctttctcgcc
      841 atcttccttg gagaatgcgc ctttttgcgc atctggcaga atttccagca ccacttctga
      901 agggcggcac aggcgagttc ccagcggcgt caccacaatc gggcgattaa tcagaatcgg
      961 gtgctgaagc ataaagtcga ttaaccgatc gtcagtaaat ttatcttccg caaggcccag
     1021 ctcctcatac ggttcgacgt ttttacgcag cagcgcgcgt acggaaatcc ccatatcggc
     1081 aatgagtttg accagttcat cgcgcgttgg cggagtttcc agataatgga taatggtcgg
     1141 ttctgtgccg ctgttgcgga tcatctccag cgtattacgc gacgtgccgc aggccgggtt
     1201 gtgataaatg gtaatgttgc tcatatcagt atctcattac aaagtgaaag agagacgtag
     1261 cgccagcgca gccagcgtca caaacagcac aggcagggtc atgataatcc ctgtacggaa
     1321 gtaatatccc cagctgatag tcatattctt ctgcgaaagt acgtgcagcc agagtagcgt
     1381 agccaggcta ccaattgggg taattttcgg tcccaaatcg cagccaatca cattggcata
     1441 aaccatcgct tctttgataa cgccagatgc cgtgctgcca tcaatggaca acgcgccaac
     1501 cagtaccgtc ggcatattgt tcataataga agagaggaag gcggtgagga atccggtgcc
     1561 gagcgtcgcg gcccacaggc cgttatccgc cagcacgttg agtacgccag aaagatattc
     1621 cgttaatccg gcattgcgca ggccataaac caccagatac atgccgagcg agaagatgac
     1681 aatctgccag ggggcaccgc gcaggacttt acccgtatta atcgcatgac cgcgtttagc
     1741 gacgacaaat aatatcagcg cgcccacagc tgcaatggcg ctcaccggaa tgccgagcgg
     1801 ttccaggacg aaaaatccca ccagcagaag cagtaaaaca acccagccag ttttgaacgt
     1861 agcaggatct ttgatcgctt ctgcgggaga tttcagcagc gccatatcgt agttctgcgg
     1921 aatatctttg cgaaaataga gatgtagcat caccagcgtg gcaacaatcg cggcgatatc
     1981 caccggcacc atcaccgagg cgtattcgcg aaagccgagg ccaaagaaat cagcggaaac
     2041 gatattcacc aggttggaga caataagcgg caggctggcg gtatcggcaa tgaatccggc
     2101 cgccatcacg aacgccagcg tcgtgccttt actgaatcct aaagccagca gcatggcgat
     2161 gacaatcggc gtcaaaataa gcgccgcgcc atcattggca aacagggcgg caacggcagc
     2221 accgagcagg acaatccagg taaacagcaa gcgaccacga ccattacccc agcgtgagac
     2281 gtgcagcgcc gcccattcaa aaaagccgga ctcatccagc agcaggctga tgataatgac
     2341 ggcgataaac gcagccgtcg cgttccagac gatattccac accaccggaa tatcacccgg
     2401 atgaaccacg cccgtaacta acgccagtac tgcgccgagc gttgcactcc agccgatgcc
     2461 taaacctttc ggctgccaga taaccaatac gatggtcagg acaaagatag cgcctgccag
     2521 taacataatg cctcccggat aaaacacatc tgaaaattca tatgtgttta gctaaatttt
     2581 taactgcaaa tgttcttact gtccccggaa cagttttgtc gagccaggtt gcggacaatc
     2641 gcctgaacct tttcctgttc acatcgccag gcctcatcaa taattttcgc cgcccatgct
     2701 ggaatatgcg gtgataagcg gtaatgaacc cacttacctt gcttgcggtc cagcaatagc
     2761 ccgctttcac gaagcaatgc caggtggcgg gagatcttgg gctgcgactg atcgagggca
     2821 gtacaaagat cgcagacgca taactctccc agctcgctga gcagtaaaac gatgcccaga
     2881 cgggtttcat cagcaagaat tttgaacaat tggatgggta acagaaatga catattgcgc
     2941 tcctgattgt ggcaggtagt gtctctcttc gaagcggata agtcaaaaac atatatgact
     3001 taacgaatgt gtaagtgcag aggaaggtaa taggtgtgaa ttttgagttg gctattcatt
     3061 tgaaaggcgg tttgaatcag gacgtaaaaa aaccgacttt gcgtcggttt tttactcttc
     3121 agcctgagtt ggtggctctg gctggagtga gaatgcccac atctaacggt tcaatactat
     3181 tagccagtgg ctaacattgc aagaattagc gatttcttca gctggcggag ggggcacttc
     3241 ggtatatatc gcaatgacga tttatatact ttcactgggt catcgtcata ttaagccttg
     3301 tccgaatccg gatgtaaaaa aaccgacttt gcgtcggttt ttttactctt cagccctgag
     3361 ttggtggctc tggctggagt gagatagtca tcatctaacg catcaatgct aataccatac
     3421 tgaaactatt gcaaggacat gctggtttta taacctgaat gtactgtatg attatccagt
     3481 tagctctgag gcattttcac tctggtaatg cgcataaacg ctttcaaagt cctggtcaga
     3541 agtacgggtg gtgccgttaa ctgatgctct ggccggagtg agatagtcgt catctaacaa
     3601 tgagacatgc gccgtgacag gcagtggatg agtaagcgga tgcattctca ctccatcgca
     3661 tggagaaaac gggtgattga taaagcaatc atcgttcttg gagcgttaat tgcgctgctg
     3721 gaactgatcc gctttctgct tcagcttctg aactgatagc ggaaacgtaa ttaagggcta
     3781 agagcacact actcttagcc ctttaacatt tagcgcatgg tcacgaactc ttctgccgcc
     3841 gttgggtgaa tggcgacggt attgtcgaag tcttttttgg ttgcgcccat cttcagcgcc
     3901 accgcgaagc cctgcaacat ttcgtccata ccaaagccaa tgccgtgaat accgacaatc
     3961 ttctcttctg gcccaacgca caccagcttc atgcggcacg gctggcggtg agtggtgacg
     4021 gcggtataca tcgcggtgaa agaggattta tacactttca cctgatcgtc gccgtactgc
     4081 tcgcgcgcct gcggttccgt taaaccaaca gtaccaatcg gcggatggct gaagaccacg
     4141 gtcggaatgt tgctgtaatc cagatgctca tccggcttgt tattaaacag gcgttcagag
     4201 agacggcgac ccgctgcaac tgctaccggt gtcagttcca ctgcacccgt gttatcgccc
     4261 accgcgtaaa taccttcaat attggtgttt tgatatttat cgacgacgat atagcctttt
     4321 tcgttagttt taacgccagc ggcttctagg ttgatgttgt cattggcagg ctcgcgacca
     4381 atcgcccaaa tcaggcaatc caccgtttca ctgcgaccat cttccagctc cagcgtcagg
     4441 ctaccatcgg catttttcac taccgctttc gggatggcgt tggtgtgcag ctgcgggcct
     4501 tcggcgttca tcacttcgac cagcgtttcg gaaatcatcg ggtcgaagct gcgcagcggc
     4561 gcatgtttac gcacaaacag atgcgttttc gcgccgaggc cgttaatcac gcccgccagc
     4621 tcaacggcga tgtaacccgc gccaacaacc gccacgcgct ctggcaaagc aggaagggcg
     4681 aagaagccat cagaatcaat accgtattcc acgcccggaa tatccgggtg gctcggacga
     4741 ccgcctgtgg cgatcagaat atgatcggcc gtgatggttt cgccgtttac ctccagcgtt
     4801 ttggcatcaa cgaagcgggc aaagcctttg attacatcaa cgttattttt accgagcacg
     4861 ttttcatagg aagtatgaat acggtcgata taggcggtac ggctggcgat caacgtttcc
     4921 cagttgaact tgttgatggt ggtatcgaaa ccataatcag ggccgtacat atggatcgct
     4981 tcacggattt gcgccgcgtg ccacatcact tttttcggca cgcagccgac gttcacgcag
     5041 gtgccgccca gctctttggc ttcaatcagt gcgcatttct ggccgtacat agccgcgcgg
     5101 ttgatggagg cgataccgcc gctgccgccg ccgatggcga tgtaatcata gtgtttagtc
     5161 atgacagagt gtccttatcg ttgattaccg cgattgtagc gcgagacgta ataggtgcca
     5221 gcaatggctg caattactcc ggcacgatcc agcttacggt ggcgtgcccg gtgcctgccg
     5281 gaaccagttt gctgtgcagc cacggcagca cgttattcat ctgctgttcc agtttccacg
     5341 gcgggttaat cacaatcatg ccggaagcgg tcatgccacg gcgatcgctg tctggcagta
     5401 ccgccagttc aatttgcaga attttgcgaa taccggtcgc ttccagatcg tggatcatgc
     5461 gcttaatttg ctgacgcagc accaccggat accacagtgc gtaagtacca gtggcgaaac
     5521 gtttgtaacc ttctgctatc ccgctgacca ccgcctgata gtcggttttc atttcatacg
     5581 gcgggtcgat aaggattaaa ccacggcggg aaaccggcgg aagtttggcc ttaagctgct
     5641 ggaaaccgtc ggctttttcg acgcgcgcac ggctgtcttt ctgaaattca gaacgcagca
     5701 acgggtaatc gctcgggtgc agttcggtca gttgcaggct gtcttgttcg cgcagtagct
     5761 ggcgagcaat caacggcgac ccggggtagt aacgcaactg accgctacgg ttgaagtgtt
     5821 ttaccacatt gatgtacgcc tccagttctg cgggcaaatc gtcctgctgc cagatacggg
     5881 cgataccttc gagatattcg ccggtacgct cggcatgttc gctgcctaac tgataacgcc
     5941 cggcccctgc gtgggtatcg agatagagaa acggtttatc tttctctttc agcgactcga
     6001 tgatcaggct ctgaacggta tgtttaagga cgtcggcgtg gttgccagcg tgaaagctgt
     6061 ggcgataact gagcatgggt aaaggtgttc cggtaagtaa aaatcggccc gtattcgggg
     6121 cgcacaaaag cgtatcagga cagtataccg aaaagaggcc gccgccgcga aagcgtaacg
     6181 tttctcattg aaattcacta cacttaaccc catgctacac acattatgta aagcgcctgt
     6241 tgagcgcttc cttaacctct ttaaccagga ctgcgcgaat gacgaatccg ttactgactc
     6301 cctttgaatt gcctccgttt tctaaaattc tcccggaaca tgtcgttcca gccgtgactc
     6361 aggcgctgaa cgattgccgc gaaaatgtgg cgcgcgtagt agcgcaaggg gcaccgtaca
     6421 cctgggaaaa tctctgccag ccgttggcgg aagtggacga tgtgttgggg cgtatcttct
     6481 ccccggtcag ccacctgaac tcggtgaaaa atagcccgga actgcgtgaa gcctacgagc
     6541 aaaccctgcc gctgctgtcg gaatacagca cctgggtagg gcaacatgaa gggctgtata
     6601 aagcgtaccg cgacctgcgc gatggcgatc attacgccac gctgaacacg gcgcagaaga
     6661 aagcggttga taacgccctg cgtgattttg aactctctgg cataggtctg ccgaaagaga
     6721 aacagcagcg ttacggcgaa attgctaccc gtctttctga actgggcaac cagtacagca
     6781 acaacgtcct cgatgcgacg atgggctgga ccaaactcgt taccgacgaa gcggggctgg
     6841 cggggatgcc agaaagcgcg ctggctgcgg caaaagccca ggccgaagcg aaagagctgg
     6901 aaggctacct gctgacgctg gatatcccaa gctacctgcc ggtaatgacc tactgcgaca
     6961 accaggctct gcgcgaagag atgtatcgcg cttacagcac ccgcgcctcc gatcaaggcc
     7021 cgaacgccgg taagtgggat aacagcaagg tgatggaaga gatcctcgcg ctgcgtcacg
     7081 aactggcgca actgctgggc tttgaaaact acgcctttaa atcccttgcc actaaaatgg
     7141 cagaaaaccc gcagcaggtg ctggatttct taaccgatct ggcaaaacgc gcgcgtccgc
     7201 aaggcgaaaa agagctggag caactgcgtg ccttcgctaa agccgaattt ggtgtcgatg
     7261 agttgcagcc gtgggatatc gcttactaca gcgaaaaaca gaaacagcac ctctacagca
     7321 tcagcgacga gcagctgcgt ccgtacttcc cggaaaacaa agcggttaac ggcctgtttg
     7381 aagtggtgaa acgtatttac ggcatcaccg ctaaagagcg taaagaggtt gatgtctggc
     7441 atccggatgt acgtttcttc gaactgtatg acgaaaataa cgaactgcgc ggcagcttct
     7501 acctcgatct gtatgcccgt gaaaacaagc gcggcggggc gtggatggat gactgcgtag
     7561 gccagatgcg taaagctgat ggttctctgc aaaaaccggt cgcgtatttg acttgtaact
     7621 tcaaccgccc ggtaaatggt aaaccggcgc tgttcactca cgacgaagtg atcaccctgt
     7681 tccacgagtt cggtcacggc ctgcaccata tgctgacccg catcgaaacc gctggtgttt
     7741 ccggtatcag cggtgtgccg tgggatgcgg tcgaactgcc gagtcagttt atggaaaact
     7801 ggtgctggga gccggaggcg ctggcgttta tctctggtca ctatgaaacc ggcgaaccgc
     7861 tgccgaaaga gttgctggat aaaatgctgg cggcgaagaa ctaccaggcg gcgctgttta
     7921 ttctgcgtca gctggagttc ggcctgtttg atttccgcct tcatgccgag ttccgcccgg
     7981 atcagggggc aaaaatcctc gaaactctgg cagaaatcaa gaaactggtt gccgtggtgc
     8041 catctccgtc ctggggccgt ttcccgcacg ctttcagcca tattttcgcc ggtggttatg
     8101 cggcaggtta ctacagctac ctgtgggctg acgtactggc ggcagatgct ttctcgcgct
     8161 ttgaggaaga gggcattttc aaccgtgaaa ccgggcagtc gttcctcgac aacattctga
     8221 gccgtggcgg ttcagaagag ccgatggatc tgttcaaacg cttccgtggt cgtgaaccgc
     8281 agctggatgc gatgctggag cattacggca ttaagggctg atcattcagt gaaaatctgc
     8341 ttaattgatg aaacaggcgc cggagacggc gccttatctg ttctggcggc ccgctggggg
     8401 ctggagcacg atgaagacaa cctgatggcg ctggtgttaa cgccggaaca tctggaattg
     8461 cgcaagcgtg atgagccaaa acttggcggc atctttgttg attttgttgg cggagcgatg
     8521 gcgcaccgac gcaaattcgg cggtggtcgc ggtgaggcgg tggcgaaagc ggtgggcatt
     8581 aaaggcgatt atttgccgga tgtggtggat gccactgcag gactggggcg cgatgccttt
     8641 gtgctggctt cagtcggttg ccgcgtgcgg atgctggagc gtaatccagt tgttgccgcg
     8701 ctgctcgacg acggcctggc gcgtggttat gcggatgcgg aaatcggcgg ctggttgcag
     8761 gagcgtttgc agttaattca cgcctccagc ctgacggcgc taaccgatat taccccacgc
     8821 ccgcaggtgg tctatctcga cccgatgttc ccgcataagc agaaaagcgc gctggtgaaa
     8881 aaagagatgc gtgtctttca gtcgctggtg gggccggatc ttgatgccga tggattgctg
     8941 gagcctgcac gcttgctggc gaccaaacgc gtggtagtaa agcgcccgga ctacgcgcca
     9001 ccgctagcga atgtcgccac gccaaacgcg gttgtgacta aagggcatcg ctttgatatc
     9061 tatgcaggta cgcccgtgta aaagtaaagc agggtagcga taacgctacc ctgctttctg
     9121 caagaataat taatggcttt ccggcgtcgc aatcatgcgt ttcagccacg gcaccatcaa
     9181 cagcatcact actgcaacgc ccagcgtaac caggccaatc ttaccaaaca cgttggtata
     9241 gacgggtaac gtctcaagcg gatcggtaat gttgtccggc actgcggtaa atgttgccac
     9301 atagccgccc agcaagaatg cggcagcctg cgtcaggaac cacatcccga gaataaagcc
     9361 catcaaatgc tgcggcacca gggcagcaat catcgccagg ccaagggcgc taataaacaa
     9421 ttcacccagg ctctggaata aatacaccag cacgataaac catggcgatg tcagcccttg
     9481 tgcatccgca aaccacattc ccgcagctgc cgccgtcaaa aagcccagtg agcacataaa
     9541 catgccgaga gtaaatttca tcggcatcga gaggtctttg cctttgttac ccagatgcgt
     9601 gtaaatgcct gccagtattg ggctggcgag taccacccag aacgggttaa gcgcctggaa
     9661 gctgaccggg tttatggaaa agccgagaat ttcatgatgc acgttgttga tggcaaagaa
     9721 gttcagcgac gttggcatct gggcgtagag aatgtaaaac accaccgctt cgagcatcag
     9781 gacaaaggcg acaaacattt tattgcgccc ggttttatcc agcttgaatg cctgacgaaa
     9841 gaagatgatg gtgacgacga tggagagaac aatcagcacc agattggcga cttctacgtt
     9901 gtgcatcagc catgcacata cgaagatcat caccacgctg ccaagtaata cgtacaacag
     9961 tttgctgaag ctcatcggct tgaagtcggg ttcagaacca atgtctttca ccattccacg
    10021 acaggcgatg taaaccagta atgcgataat taaccccgcc ccgcacaggt tgtaggtgac
    10081 tgaataaccg tatctatcag cgatcacagg ggccagcgat aacgctatca acgagccgat
    10141 attgatcgac atatagaaca gggtgaatgc gccatcaagc cgcggatctt tcggcggata
    10201 gcacttcgaa agcaagctgg ctgggttagc tttaaacagg ccgttaccga cagcgatagt
    10261 ccccagggcg atgaaaatca ggtcaggctt aagtagcgac atgccggtca tgaagtagcc
    10321 aatcgccagc acaagtgctc cgagaacaat ggtgcgtttg gtccccagca ggtggtcgcc
    10381 gacatagccg ccaatggaaa tgaggccata gaccagcgca gcaaaagcac caaaagtgac
    10441 aaaagcctgc tcttgcgaga atccaagctg tttaacgaag aaaaccgcca gtacgccctg
    10501 cacgccgtag tagccgaatc gctcccataa ctcgacaaaa aagatcatga aaaatgggcg
    10561 aggttgctgc agcatcccca tgggtgttgt tgtattcata tttattaacc ttccaatacc
    10621 atcctgaaaa gtatgaacgc gtaagggata acgcgcgaaa aagtttacat ctgtcagaga
    10681 ttcttaatat tgatgcggtg aatagattaa tacactatta caacagaaaa taaccagctg
    10741 atcacactaa tgatggaaaa tatgatagtg ttaaatgttg tattggcagg agattttgat
    10801 tgcgtacgca gatcgtaggc cagataaggg gtttacgctg catcaggtaa caaggcgcgc
    10861 caaaaagcaa aacgcccgca tatgcgggcg ttcaggaaca cgtcgtgggg ggatgattat
    10921 tcttcttcgt cgcgcagcgg aacaatcagc atatcaacgt gaacggtgtt gatcagctga
    10981 cgtgcggaag acattagttt gctccagaag tcctggtggt gaccacaaac caccagatcc
    11041 atatcgtatt tcttgattgc atcgaccaga acctggccca ggtcgccgct gccgctcagg
    11101 gtttcagtga ttgggtagcc tgcattagcg gaaagctcgg tcagcgcatg atgtgtctct
    11161 tcagagatgc gtttctgcat atcacccaga ttcacatcaa taagcccggt gtataggtca
    11221 gagtagttta catctacgtg gatcagagaa actttcgcat tgtaggggcg agccatagag
    11281 actgctttct ctaccagaac tttgctttcc ggggagagat cgaccgcgat gagaatgtgt
    11341 ttataagcca tagtgttact ccttccataa agttgtcgat gactggccag ctagcgtttc
    11401 ttgtgccgtt acgtcacccg cgtcctgcga actacgtgcg cggggctaac ccagccgtaa
    11461 agattcagtg ttaagaccat ccttacctta tagcgacccg gatgatccgt caatccgcct
    11521 tgcttacccg ttagataaac aaattttcga catgaatata ttgatagtgg ttaaccttct
    11581 ggaaaaaaaa caacctgatc tcctacacta tctatagagc cgctcggatg ttggtcacca
    11641 caaaagcggt ttctccagga cctggtgtta actgattggc agcgtatcgg gagcggtagc
    11701 cccggggtgg aactccgcgg gcaggtcgcc ggggaggagg tatgataagc accgtcgcat
    11761 tattttgggc tttatgtgtc gtttgcattg ttaacatggc gcgctatttc tcatcactac
    11821 gcgcgttgtt agtggtactg cgtaactgcg atccattgct ctatcaatat gttgatggag
    11881 ggggcttttt tacctcacat ggccaaccca acaaacaggt gcgtctcgtt tggtatatct
    11941 atgcccaacg ttatcgcgat catcacgatg atgaatttat tcgccgctgt gagcgggtgc
    12001 gtcggcagtt tattctgacc agcgcattgt gtggtctggt ggtggtcagc ctgattgcat
    12061 tgatgatttg gcattaagca cttcagcaat aataacgcat aaaaaaagcg ggccagtaaa
    12121 ctgacccgct cgagactgat tacaaacgca aaattgcctg atgcgctacg cttatcaggc
    12181 ctacataatc tctgcaatct attgaatttg catgtttttg taggccggat aaggcgttca
    12241 cgccgcatcc ggcatgaaca aagcgcacta tgtcacaatc tgaagcgggc cagctgaccc
    12301 gctcgttttg gtgcgtacga ttacaggaac tgcaaggaga gccagtacag cccgccggaa
    12361 agcagtaccg cagccggaag ggtaaacacc caggccatca gaatactggt tacggtttta
    12421 cgctgtaagc cgccgccatc taccaccatc gtccccgcga cagaagagga gagtacgtga
    12481 gtagtggaaa ccggcatccc ggtataactc gccaggccga tagacactgc cgccgtcatc
    12541 tgggcagaca tcccctgagc gtaggtcatg cctttcttac cgattttctc accgatagtc
    12601 gttgccacac ggcgccagcc aatcatcgta ccgataccta acgccagcgc gaccgccatg
    12661 atgatccaca ccggtgcata ctcgatggtg ctaagcatgt cggacttcag tttcttcaac
    12721 aggcgctgat cgtcagcact cacgccaggc atcttcacca ctttgtcgat agtgtcagaa
    12781 acgcacagca taatgcggcg catctggcta cgttgatcaa gcgacagctt gtcgtagctt
    12841 tccacatcgg tggtcaacat acctttcagg cggttgagcg cgttaatggt attcgacgga
    12901 tggcagtgga actccgcagg ttgcgttgcg ccagcttccg gagccggtac taactgatca
    12961 gcaccggtag cctgtttgag cagcgcagga tgctgctcaa agtaagcttc gacgttgttg
    13021 atggcatcac gggtacgggt gatttcgtag ccagtggcat tcatgttcac cacgaagcct
    13081 gctggcgcga cgccaatcaa taccaacata accagaccaa tgcctttctg accatcgttc
    13141 gcgccgtgcg aaaacgccac gccgatagcg gaaaggatca gcgcgatacg tgtccagaac
    13201 ggcggctttt tcttgccgtc tttcttttca cgctccgctg gggtcaggtg gatacgggcg
    13261 cgtttcttgg tgccgctcca gtagcgacgc agcaagaaaa tcagaccgcc agcaaacacc
    13321 aggccgacaa taggggaaac gatcagagaa ccgaaaatac ttaatacttt cgggatattg
    13381 agtgcatcca ccactgacgt cccggtcatc aacgcattgg ttaaaccaat cccgatgatc
    13441 gcgccaatca gcgtatgaga gctggatgca ggtaaaccaa agtaccaggt acccaggttc
    13501 cagataatcg ccgccagcaa catagagaac accatggcaa ggccatgaga cgatcccata
    13561 ttaagcagca gatccgtcgg cagcatatgc acaatggcat aggcaacact cagaccaccc
    13621 agcaaaacac ccaaaaagtt gaacaccgcc gccataacca cggcgagctg agaacgcatc
    13681 gcgcgggtat agataacggt tgccacggcg ttggctgtgt catggaaacc attgatggct
    13741 tcgtagaaca gcacaaaagc cagtgcaagc aataataaca gcccggtatg caaatccagg
    13801 ccagcaaaca aatgtagcat aggacgttac gccattttga ggacatgaac gcggcgcatt
    13861 atcagtgact ttcgcggcgc gggcaaagtg aaatatagac tttttttgat ttgcctcctg
    13921 tatggatttc actcaaaaaa taattatctt atataattca ggcaaatact tccttttagt
    13981 aatattgatg ctggtgcgac cactgaggaa tctttacaat tcacgcccgt tttttctaag
    14041 aggagcgcaa cgtggaaagg tttgatgcca ttattatagg cgctggtgcg gcgggtatgt
    14101 tctgttctgc gctggcaggt caggcaggac gccgggttct gctgatcgat aatggtaaaa
    14161 aaccagggcg caaaatcctt atgtctggcg gtgggcgctg caactttacc aacctttatg
    14221 tcgaaccagg cgcttatctg agccagaatc cgcatttttg taagtctgca ctcgcacgtt
    14281 ttacccagtg ggatttcatt gatctggtca ataaacacgg catcgcatgg cacgagaaaa
    14341 cgttagggca actcttctgc gatgactccg cgcagcagat tgtcgacatg ctggtggatg
    14401 agtgcgagaa gggcaatgtg accttcagat tgcgtagcga agtgctgagt gtggcggagg
    14461 atgaaacagg cttcacgctt gatctgaacg gcatgactgt cggttgcgaa aagctggtca
    14521 tggcgaccgg tgggctgtca atgccggggc tgggcgcgtc gccgtttggt tataagattg
    14581 ccgaacaatt tggcctcaac gtgctgccga cccgtgcggg tctggtgcca ttcactctgc
    14641 ataaaccgtt gctcgaagag ttacaggtgc tggcgggcgt ggcggtgcct tccgtgatta
    14701 ccgctgaaaa cggcatcgtt ttccgtgaga acttactctt cacccatcgc ggcttgtctg
    14761 gaccggcggt gttgcagatt tccagctact ggcaaccggg tgaatttgtc agtatcaatc
    14821 tgctaccgga tgtggatctc gaaaccttcc tgaatgagca gcgtaacgca catccgaatc
    14881 agagcctgaa aaacacactg gcggttcatc taccgaagcg gttggttgaa cgcttacagc
    14941 aactcgggca aatcccgaat gtttcgctaa aacagctcaa cgtgcgtgac caacaggcac
    15001 tgattagcac attgaccgac tggcgcgtac aacccaacgg cactgaaggc tatcgcactg
    15061 ccgaagtgac gctcggcggc gtggacacca acgaactctc ttcacggacg atggaagcgc
    15121 gcaaagtgcc tgggctgtac ttcatcggcg aagtgatgga cgttaccggc tggctggggg
    15181 gctataactt ccagtgggcg tggtcgagtg cgtgggcttg tgcgcaggat ttgattgcag
    15241 cgaagtcgtc ctgacctgtt acctaaagat gaaaattatt agagccgcat taaaaatgag
    15301 ctaattttga tagtggctat cttgtgatta ttttctaatg agcccgtgaa ctgaaaccct
    15361 ccaggcttaa tataaggtgg aaggaaaggt gattgaaaac tcacccagtg gccagatctt
    15421 tataaaatat gaacaggctg aaaaagacag taactttgtt gtttttttat ccttaaccac
    15481 tatgcattaa tgctgcgtta tttcatgatg cctaagaaaa accagcgtta cgcaaatggt
    15541 caacgctggt tttatccggt acgttgcaat tattttttag cagaacccgc ttctaatatt
    15601 gaaacgattg agaacaacgt aaagcatacc gctcccaaac cgaccagaac tcgcgaagga
    15661 ataaaatatc ccatgtcggt ctgcgccatt tccgcaagaa atgatgccag gaaaaggcaa
    15721 aacaggcagg tgaagacggg tatcatcggt attctgttgg ctaacgaaca ggtacgtctc
    15781 cagaccagtg ccagtagcca gacttttgag aatatgctgt aacagatcat tccgaggcaa
    15841 ataagaataa tgccgggagc cagtcgggcg cttgcatcgg aactgactaa gacgtatatc
    15901 ccctgcagta ccgtgattga gccgagaaaa ataacccaat aacaccacag ccagtgttct
    15961 ttagttgaaa acgtattacg tgtttgatgg acaattgtgg caacaaggcc aattagacag
    16021 gcacagattg cggttaaccc aagcaatacg tgacccgcga cataatgcgg agttatgtca
    16081 gcactacgta acagcgtaat agaccaaatg aaaccgagca gggtgagtaa aacgggaacg
    16141 gcaatgaggc aattaccgat taatgaagaa taagcctgta ccggcgttcc atcgctcttg
    16201 ctccccgctg catttttggg aatgagcaga aagtgaccgg atgacgctgc caccgtcgat
    16261 acacaggcgg caatcatacc aacgccgaaa ataacatggc cggcgacaaa ctcgtctgcc
    16321 atcacatcat tgcctgctaa cagtgcccaa ccccatatca tggtgataat tgaccccgca
    16381 tagccaataa tggggaacaa tgtattgtaa aacgtattaa cgccgcgcgt gagctgcgaa
    16441 atgataataa atgcggtagt gaataatgcc aggcatatgg ccgccagaga aatcagcaca
    16501 tgacccgcga cgaaatactc gctttgccct gaactttcca gcacaaagcc acctaacgcg
    16561 atgcaaatta atcccataat caaagggatt aatttgaaaa gccacccgat atatatgttc
    16621 actttaaact cctcaggtat ccctgctaat aatgaaatgt gcattaatga taatttcagc
    16681 ctgttaatat aacgacgaga tatcattaaa gacaaatgca tagcataact atagcggggt
    16741 ttactgtgcc gagtgccatt ttttaccggt taaaaggcca tgataaataa atatttatcc
    16801 taagattaat tcttattctg ttgttttttt acaattatct tccagggatg gattgttttc
    16861 cattatttag taaaagaccg gatagtctcg gtgcttaaaa tcatcgaaca tataagtatt
    16921 attgctttgt atcatcgtaa atatgtcttt cagacatgta ttgtttattt tgttactaac
    16981 aagtaaaaat attacaggtt atttatgaaa gtatttcaca atatatttaa gcacttctct
    17041 tcaaaccatc aagataagca ttcagataaa gttaacagtc atcaacacca tggcaaggtt
    17101 gataaaaaat atcgatcaaa cacagttgaa gttaatatgc tgaatgactt ttcgcaaata
    17161 gataacgatt taggcctgaa tatcatctat ttcctgcaac atggtcactg gagggtaaat
    17221 gaacatagtc ttcagatgga gaaaatatgg ttttacaata gtgagccatc tatagatatt
    17281 caagaatata atagtttcgc cgataatact actaacacgt ttatctttac aattatacca
    17341 gacaataacc atgtgataaa attatcgtct cccattacag ttaagattga gtgtaaggat
    17401 ggttattatt ttattaattt ctccggagat aaatcagaca taatttataa atcagacata
    17461 acttataaag ttaatgggct ttctatcata cctgaaaatt tctttactct tcttagcggt
    17521 aattttaaag ctgactggcg ttgggatgtc tctaaagaga cgtttcctaa ggataagttt
    17581 gatagctatg ttaaatctgt gttttctaaa atagactttt ataaacaatg tggtgtgatt
    17641 aacccacaaa atgccaacac ggcttatttt ggcgatacag atggaagagt aggcgctgta
    17701 ctttatgcat tgctcgtttc agggcatata ggcatcaggg aaaagggttg gagtttatta
    17761 tgtgaattat taaaacacga aaacatggct tcatctgcat acatacataa aaataataaa
    17821 ttgctctaca atttattaaa tacgagagac atgatattga atgagttgca tcaacatgtt
    17881 tttttaaagg acgatgcgat aaccccttgt atcttccttg gggatcatac aggcgataga
    17941 tttagtacta tttttggtga taaatatatc cttactttgc taaattctat gcgaaatatg
    18001 gatggtaata aagatagccg tattaataaa aatgtcatcg ttctggctgg aaatcatgaa
    18061 atcaatttta atggtaacta tactgcacga ctagaaaatt ataaactctc tgccggtgat
    18121 acgtataact taattaaaac gctcgatgtt tgtcattatg actcagaaac gaaagtttta
    18181 accagtcatc acggaataat aagaaatgaa gaaaatgaat gttattgtct cggtgctttg
    18241 caggttccat ttaatcagat gaaagaccca attgatcctg aggaattagc aaatatattt
    18301 aataaaaaac ataaacaaca catggatgat cgtcttatcc atctaattag aagcaatgct
    18361 gtagcatcca cacctgtata tgaaaattat tttaataaca ctactgcttt cagaccaaaa
    18421 cctgaagata tcttcaaatg tggacagacg ctcaaaaaaa ctaaacagaa atatggccat
    18481 tatggattag gggtagatca gcatcaaaaa attgataatt ataccatggg gctaaattcc
    18541 tggaagatag cacctaacga acgtggtgat aaaaaaggtg tcccagggct tagttgtttt
    18601 caacctcaat aaaacttcat gagtgctgtg gtatctcttt ttaattttgc cagtatttca
    18661 taggttttct taatcgattg tagaggcatg ttctgatgac gcctctacaa tctttaggga
    18721 ctttctctgc tatgagataa gaaatttcat attatgcttt aaggctgaat gatttgtgca
    18781 aaatataatt attttcccat cgaacatgag gtgtatgtca taagattaaa tgttaactat
    18841 gtattcctcg cggtaaataa tatgaggtaa aagatgaaaa ttggaactgt ggcaggcact
    18901 aacgacagta caacgacaat cgcaacaaat gatatggtgc aagagcatgt taccaacttc
    18961 actaaagaac tgtttgggta tattgcaaat ggtataggtg atgatatatc aagtattgcc
    19021 aggacaatgc tcggtgaggt tgtggaaaaa atagatgatt ggcaaataga acggttccaa
    19081 caatctattc aagatgacaa aatttcattt accatacaaa cgaatcattc agaaaaatat
    19141 agtatgctat caggcatgcg cgctcatatt ctacgtagga ataactgtta tcagtttatt
    19201 gtgacaatta atagtaaaaa ttacggttgc cctcttgata ataccgatat taattggtgc
    19261 agcattgttt atttacttaa caatatgaca gtaaatgata atgctaatga tgttgcagtt
    19321 actgagtcgt acaaacccat ctggaactgg aaaatatcgc aatacaatgt atttgatatt
    19381 aaattcgaaa caatgataaa accccagttc gctgacagaa cctatttctc caattgttca
    19441 cctgttgatc ccacaagtac cagaccgacc tattttggcg ataccgatgg ctcagtcggt
    19501 gcagttttgt acgcgctttt tgcaacggga catttgggaa tcatggctga aggagaaaat
    19561 ttccttagcc agttgcttaa tatagaagat gaagttctca acgtattgct aagggagaac
    19621 tttaatgagc aacttgacac taatgttaat ataataatca gtatcctgaa cagaagagac
    19681 attgttttag aaagtcttca gccttatttg gttattaata aagacgcagt cacgccatgt
    19741 acgtttctgg gcgaccagac tggcgatcga tttagtaata tctgtggcga taaatttatc
    19801 atcgatttat taaaacgcat aatgagtatc aatgaaaacg ttcatgtatt agctggaaat
    19861 catgaaacga attgcaatgg taactatatg cagaacttca cgcgcatgaa accacttgat
    19921 gaggatacct actctgggat aaaagattat cctgtatgtt tctatgaccc caaatacaaa
    19981 ataatggcaa atcatcatgg gataactttt gatgatcagc gaaaacgtta cattatagga
    20041 ccgataacag tctctagtga tgaaatgacc aatgctctcg atccggtcga attagcggca
    20101 attatcaata aaaagcacca tgctgtaatt aatggcaaaa agtttaaaaa tagccgggct
    20161 atctcttgtc gatcctttaa tcgttatttt tctgtttcga cagattacag acctaaactt
    20221 gaagcactat tagcatgctc acaaatgtta ggtataaatc aagttgttgc acataacggt
    20281 aatggtggaa gagaatgtat aggtgaaacg gggacagttt taggtcttaa cgcccgtgac
    20341 agtaaacatg ctggaagaat gttcagcatg cataattgcc aaattaaccc tggtgcaggg
    20401 cctgagataa ccactccctg gaagtcttac cagcatgaaa aaaacagaaa tggacttatg
    20461 cctttgatca gaaggcgcac aatgttacag ctctgaacca tagacaacat gaatattaat
    20521 gttactatct ggttgagact gttgataaaa cgtaaaaagg aatgctctgt tcctgcaact
    20581 gaacaggcgt gaaacgctat ccaacaggat ggataccgtt ttgtcagcca gttttataga
    20641 tcacacttat gagtatgcgt gtttcattta cggccaggaa tgctgatgat gacaacatta
    20701 ctacccgttt tcactaagcc ttccccgttg gcgctcaatg ctctgcgcgc tggtcgtatt
    20761 tgtcgtttcc ttcttatccc ggatggaaga atccgctgaa cgtcgttcgg cggcagttgt
    20821 aacacgcgtc gtttgcttgc agggattatg gcgggtcgat tatggataag agtaagcgcc
    20881 atctggcgtg gtgggttgtc gggttactgg tggtggctgc cgtcgtggcg tggtggctgt
    20941 tgcgcccggc gggtgtgccg gaaggctttg ctgtcagcaa tgggcgcatt gaagcgacgg
    21001 aagtggatat cgccagcaaa attgccgggc gtatcgacac cattctggtg aaagaaggcc
    21061 agtttgttcg cgaaggcgaa gtgctggcga agatggatac tcgcgtgttg caggagcagc
    21121 gactggaagc catcgcgcaa atcaaagaag cacaaagtgc cgttgctgcc gcgcaggctt
    21181 tgctggagca acggcaaagc gaaactcgtg ccgcgcagtc gctggttaat caacgccagg
    21241 ccgaactgga ctccgtggct aaacgtcata cgcgttcccg ctcgctcgcc caacgagggg
    21301 ctatttctgc gcaacagctg gatgacgatc gcgccgccgc tgagagcgcc cgagctgcgc
    21361 tggaatcggc aaaagctcag gtatcggctt ctaaagcggc tatagaagcg gcccgtacca
    21421 atatcattca ggcccaaact cgcgtcgaag ccgcgcaagc cactgaacgg cgtattgccg
    21481 cagatatcga tgacagtgaa ctgaaagccc cgcgtgacgg acgcgtgcag tatcgggttg
    21541 ctgagccagg ggaagtgctg gcggcaggcg gtcgggtgct gaatatggtc gatctcagcg
    21601 acgtctatat gactttcttc ctgccaaccg aacaggcggg cacgctgaaa cttggcggtg
    21661 aagcccggct gatcctcgat gccgcgccag atctgcgtat tccggcgacc atcagttttg
    21721 tcgccagtgt cgcccagttc acgccaaaaa ccgtcgaaac cagcgatgaa cggctgaaac
    21781 tgatgttccg cgtcaaagcg cgtatcccac cggaattact ccagcagcat ctggaatatg
    21841 tcaaaaccgg tttaccgggc gtagcgtggg tgcgggtgaa tgaagaactt ccgtggcctg
    21901 acgacctcgt ggtgaggttg ccgcaatgac gcatctggaa ctggttcccg tcccgcctgt
    21961 cgcgcaactg gcgggcgtga gccagcatta tggaaaaacc gttgcgctga acaatattac
    22021 tctcgatatt ccggcccgct gtatggtcgg gctgattggt ccggatggcg tcgggaagtc
    22081 gagcttattg tcgttgattt ccggtgcccg cgtcattgag cagggcaatg tgatggtgct
    22141 gggcggcgat atgcgcgacc cgaggcatcg ccgcgacgtc tgcccgcgca tcgcctggat
    22201 gccgcagggg ctgggcaaaa acctctacca caccttgtcg gtgtatgaaa acgtcgattt
    22261 tttcgctcgc ctgttcggtc acgacaaagc ggagcgggaa gtgcgaatta atgagctgct
    22321 gaccagcacg gggttagcac cgtttcgcga tcgtccggca gggaaactct ccggcgggat
    22381 gaagcaaaaa cttgggctgt gctgcgcgtt aatccacgac ccggaactgt tgatccttga
    22441 tgagccaaca acgggggttg acccgctctc ccgctcccag ttctgggatc tgatcgacag
    22501 tattcgccag cggcagagca atatgagcgt gctggtcgcc accgcctata tggaagaggc
    22561 cgaacgcttc gactggctgg tagcgatgaa tgccggagaa gtgctggcaa ctggcagcgc
    22621 cgaagagcta cggcagcaaa cgcaaagcgc tacgctggaa gaagcattta taaatctgtt
    22681 accgcaagcg caacgccagg cgcatcaggc ggtagtgatc ccaccgtatc aacctgaaaa
    22741 cgcagagatt gccatcgaag cgcgcgatct gaccatgcgt tttggttcct tcgttgccgt
    22801 tgatcacgtt aatttccgca ttccacgcgg ggagattttt ggttttcttg gttcgaacgg
    22861 ctgcggtaaa tccaccacca tgaaaatgct caccggactg ctgcccgcca gcgaaggtga
    22921 ggcgtggctg ttcgggcaac cggttgatcc aaaagatatc gatacccgcc gtcgggtggg
    22981 ctatatgtcg caggcgtttt cgctctataa cgaactcacc gtgcggcaaa accttgagtt
    23041 acatgcccgt ttgtttcaca tcccggaagc ggaaatttcc gcaagagtgg ctgaaatgag
    23101 cgagcgtttt aagctcaacg acgttgaaga tgttctgccg gagtcattgc cgctcggcat
    23161 tcgccagcgg ctttcgctgg cggtggcggt gattcatcgc ccggagatgt taatcctcga
    23221 tgagcctact tctggtgtcg atccggtggc gagggatatg ttctggcagt tgatggtcga
    23281 tctctcgcgc caggacaaag tgactatctt catctctacc cactttatga acgaagcgga
    23341 acgttgcgac cgcatctcac tgatgcacgc cggaaaagtg cttgccagcg gtacaccgca
    23401 ggaactggtt gagaaacgcg gagccgccag tctggaagag gcatttatcg cctatttgca
    23461 ggaagcggca gggcagagca acgaagccga agcgccgccc gtggtacacg acaccaccca
    23521 cgcgccgcgt cagggattta gcctgcgccg tctgtttagc tacagccgcc gcgaagcgct
    23581 ggaactgcga cgcgatccag tacgttcgac gctggcgctg atgggaacgg tgatcctgat
    23641 gctgataatg ggttacggca tcagtatgga tgtggaaaac ctgcgctttg cggtgctcga
    23701 ccgcgaccag accgtcagta gccaggcgtg gacactcaac ctctccggtt cccgttactt
    23761 tatcgaacag ccgccgctca ccagttatga cgagcttgat cgtcggatgc gtgcgggcga
    23821 tatcacggtg gcgattgaga tcccgcccaa tttcgggcgc gatatcgcgc gtggtacgcc
    23881 tgtggaactc ggcgtctgga tcgacggagc gatgcccagt cgtgccgaaa cggtaaaagg
    23941 ttacgtgcag gcaatgcacc agagttggct tcaggatgtg gcgagccgac aatcgacacc
    24001 cgccagccaa agcgggctga tgaacattga gacgcgctat cgctataacc cggacgtaaa
    24061 aagcctgcca gcgattgttc cggcggtgat cccgcttctg ctgatgatga tcccgtcaat
    24121 gctaagcgcc cttagcgtgg tgcgggaaaa agagctaggg tcgattatca acctttacgt
    24181 gactcccacc acccgtagcg aatttttgct tggcaaacag ctgccgtaca tcgcgctggg
    24241 gatgctgaac tttttcctgc tctgcggcct gtcggtgttt gtgtttggcg taccgcataa
    24301 aggcagtttc ctgacgctca ccctagcggc gctgctgtat atcatcattg ccaccggaat
    24361 ggggctgctg atctccacct ttatgaaaag ccagattgcc gccattttcg gaacggcgat
    24421 tatcacgttg atcccggcga cacagttttc cgggatgatc gatccggtag cttcgctgga
    24481 agggcctgga cgttggatcg gcgaggttta cccgaccagt cattttctga ctatcgcccg
    24541 cggaacgttc tcgaaagcgc tggatctgac tgatttgtgg caacttttta tcccgttgct
    24601 gatagccatc ccgctggtga tgggcttaag catcctgctg ctgaaaaaac aggagggatg
    24661 atgcgccatt tacgcaatat ttttaatctg ggtatcaaag agttgcgcag tctgctcggt
    24721 gataaagcga tgctgacgct gattgtcttc tcgtttacgg tgtcggtgta ttcgtcagcg
    24781 accgttacgc caggatcgtt gaacctcgcg ccgatcgcca ttgccgatat ggatcaatcg
    24841 cagttatcga accggatcgt taacagcttc tatcgtccgt ggtttttgcc accggagatg
    24901 atcaccgccg atgagatgga tgccggactg gacgccggac gctatacctt cgcgataaat
    24961 attccgccta attttcagcg tgatgtcctc gccggacgcc agccggatat tcaggtgaac
    25021 gtcgatgcca cgcgcatgag ccaggcattt accggcaatg ggtatatcca gaatattatc
    25081 aacggtgaag tgaacagctt tgtcgcgcgc taccgtgata acagcgaacc gttggtatcg
    25141 ctggaaaccc ggatgcgctt taacccgaac ctcgatcccg cgtggtttgg cggggtgatg
    25201 gcgatcatca acaacattac catgctggcg attgtattga ccggatcggc gctgatccgc
    25261 gagcgtgaac acggcacggt ggaacactta ctggtgatgc cgataacgcc gtttgagatc
    25321 atgatggcga agatctggtc gatggggctg gtggtgctgg tggtatcggg attatcgctg
    25381 gtgctgatgg cgaaaggtgt actgggcgta ccgattgaag gctcgatccc gctgtttatg
    25441 ctgggcgtgg cgctcagtct gtttgccacc acgtcaatcg gcatttttat ggggacgata
    25501 gcgcgttcaa tgccgcaact ggggctgctg gtgattctgg tgctgctgcc gctgcaaatg
    25561 ctttccggtg gatccacgcc gcgcgaaagt atgccgcaga tggtgcagga cattatgctg
    25621 accatgccga cgacacactt tgttagcctc gcgcaggcca ttctctaccg gggtgccgga
    25681 ttcgaaatcg tctggccgca gttcctgacg ctgatggcaa ttggcggcgc gtttttcacc
    25741 attgcgctgc tgcgattcag gaagacgatt gggacaatgg cgtaaggaag ggaaacttga
    25801 cttcatactg tgatgtcata ctattgatgt cataaagtga agtcataagg acatgccatg
    25861 agaaccaagg tacaggcttt acggaagaaa aaaaaataca ttggatcaaa tatttaaaac
    25921 tcctgttcct caagggatca agtggtctga tatagagtca ctggttaaag cattaggcgg
    25981 agaaattaag gaaggaagag gttcgcgttg taagttcata ctaaatatga gcgttgcgtg
    26041 tttccatcgg cctcatccgt cgccagatac tgataaaggc gctgtagaaa gcgtgcgtga
    26101 ctggttacta agtatagggg taaaaccatg atcaaactga agacgccaaa ctctatggag
    26161 attgccgggc aaccagctgt tattacctac gtgccagaac tgaatgcatt tcgtggcaaa
    26221 ttcctgggct tgtcaggtta ttgtgatttt gtctctgaca gcattcaggg gctgcaaaaa
    26281 gaaggggaac tttccctgcg agagtatctt gaggattgta aagctgcggg tattgagccg
    26341 tatgcgcgta cagaaaagat taagaccttt acgttacgct atccagaatc gctgagtgaa
    26401 cgccttaata atgcggctgc gcagcaacaa gtctctgtta atacctatat tattgaaaca
    26461 ctgaatgagc gtttgaatca tttataaatt ttagcggcgt acattacgca gaatatctgc
    26521 ctttacagga tctatctatg caactgaact taattttata aattaagttc agttaatatc
    26581 ttttctttcc tcgtttcaga aataactcaa tcttccttcg gcaagcactg caaatgccca
    26641 tgccgcacgc cgcgctgggc gataacgtca tcggcaaaat gctgcacgtc gcccatatca
    26701 cctttcagca cggcgatttc cagacagtcg tcgtgattga tatgcacatg cagcgtggcg
    26761 acggagagat cgtggtgatg gtgctgggtg gagacaatgc ggctggctaa gtcgcgtttt
    26821 tcgtgttcat acacatagga gagcaccgcg aaaccttgcg tgccgtgctg ctgagtggcc
    26881 tcctgggcca gggcgctacg cagaatatcg cggatagctt cggaacggtt gttataacca
    26941 cgacgctggc tcaggctgtc cagcgtctcc agtaaatcgt catcaagcgt gatggtgact
    27001 cgttgcattt gcgttaaacc ttttctgtgg tgcgacggcg cacggggaat gcgggtaata
    27061 ccgcgttttg tagcacacgt ccggcgtcag aggaaaaggt taatttatct cccaccacct
    27121 gggtttcgac gatttgtccg ttgtccatca ccatcacccg ctgacagaag cgttccacca
    27181 ggcgtaagtc gtgggtgatg aacaggcagg cggtgccaaa ctgttgttgt agctttttca
    27241 gcaggcgaat gacgcccgcc tgtaacacga gatcaaggtt agaaacggct tcatccagaa
    27301 tcagtagttt cggttcgacc gccagcgcgc gagccaggca gacgcgctgg agctggccgc
    27361 cgcttaactg cggtgggcgt ttgtcgagaa cgctgtcatc gagatcgacc gccttcagca
    27421 tttcgctggc gcgcgccagt tgttcggatt ttttcagtga tagcaggtgg cgcatcggtt
    27481 cacgcaggat ctcgcgcacg gttttgcgcg gattcacggc gctgatggag tcctgaaata
    27541 ccatctgaat atcgcggcgg aacgctttac gctgggcgcg attgagtttc gccagcggtt
    27601 cgccacgcca gctaatattc ccctgcgcgg gcgattctaa acccaccagc aaccgcgcga
    27661 gggtactttt cccgcagccg ctgcgcccca gcagggcgac ggtttcgccg cttttcaggg
    27721 tcagggaaac gttattcagc accgcctgat gttgatgttt tccgttaaat ccaccgtggg
    27781 catagtgatg ggaaaggccg gagacgttaa gtaaagtcat gatgccagct ccataccgta
    27841 gagggcgaga tgagcggaaa ccaggctgcg cgttaccgca tgtttggggg cgttaaacag
    27901 cgtttctaca tcgccctgtt cgacaatttt accttgtgac atcaccgcca cgtcatccgc
    27961 cagacgcgcc acaacgccca tatcatgggt gaccagcagc attcccggcg cttgtttttg
    28021 cataatgctt tccagcagat cgaggatgcg cgcctgtgct accacgtcga ggtcggtggt
    28081 cggttcatcg gcgatgataa acggtgattc acacagcacc gccatcgcaa tcatcatgcg
    28141 ctgcaacatg ccgccgctca tctcgaacgg gtacagcttc agcacgcgcg cggcgttttc
    28201 cagccccacc gcttctatgg cagcggtaag cgtagcgtca tcggcgggtt tccctaacgc
    28261 caggcaggtt tcccgcgcgt gggtgtgcat ggtgtgtagc ggattaaagg cgctgcgcgg
    28321 gttctgcatg atggtggcaa ttttgatgcc gcgcagggcg cagggataaa ccgctttgcc
    28381 atcggctaaa atttcccccg ccgtctggcg aacgccagcg ggcaaaatgc ccagcgtcgc
    28441 ggcgcaggtt aatgatttcc cgctgccgct accgccgact aacgccagca cgcgcccgcg
    28501 ttgcagggtt aacgatacac cgtgtaccag cggctgcgcg gcctgtagcg cgatattacg
    28561 tagttcaatc tgttgcggca ttagtgtgcg tgctccgtca ccagatgagg atccagatga
    28621 tcgcgcagtg cgtcacccac caggttaaag gccatcacgc tgataaacag cgccagcccc
    28681 ggccagaaca tttgcagcgg ctgggtccag atatactggc gcgcgtcgtt aatcatcacg
    28741 ccccattcgg cggtcggcgc ggtcacaccg aggccaagga aagacatccc cgcgacgtgc
    28801 agcatcatat ggccgatatc cagcgttgcc agcaccagca gcgaagggat caccgcgcct
    28861 gccagatgat cgacaaacac ccgcacatgg cccgcaccgg aaagccgtga cgccagcaca
    28921 aactcgcgtt ggcgtagtga aatcaccaag ctgcgcacca tgcgtgcata ccacgcccag
    28981 tgcgacaggg cgatggcgat aattacgttg gtcagcccgg tgccgagcac gccaaccata
    29041 aagaacgaca gaatcgaggt cgggaaggtc ataaacatat cggcgacgcg catggtggcc
    29101 tgatcaacgc gcccgccaat caacccggcg ctgccgccaa taaccagccc taatgtcagc
    29161 accagcagca ggcaggccat taccgaaccg agcgacacgc gggtcgctgc catcagccgc
    29221 gagaaaatat cgcgacctaa gtgatcggtg cccagccagt gctgcgcatc cggcgaaagc
    29281 aggcgcgacg gcaaatcaat cgcctggggg tcatacggca gccaccactg gctggtgagc
    29341 gcaatcagcg ccagcagggc gataatgatc agcgccaggc gtaccgacca gcgggaagag
    29401 aggaaaaagt tcacgcgtgc gctccttcat gacgacgaat gcgcgggtcc agcgcggcgt
    29461 tgagcaaatc gacaatcaaa ttacagacca caaaaaccac caccatcatc agcgtaaagc
    29521 actgaatcac cggatagtca cggttaaaaa tcgccgacac cgcatagcgc ccgacgcccg
    29581 gccaggcaaa gatgttttcg ataatcatcg tcccgccaat cagttcgccg atgtgcatcc
    29641 ccacggcggt gatcatcggc agcgaggcat tgcgcagaat atggcggcgt tcggtctgtt
    29701 tgtcgttcag gccgcgcaga cgcgcccagg tgacgtgacg ctgaccggcg acgtccagca
    29761 tactggcgcg cagcagtcgc gcgttaatcg ccagcgacat aaaggcaatg gaaaccgcag
    29821 gcaaaatgat gtgctgccag ccgccgtaac ccatcgcggg tagccattgc agatacaccg
    29881 aaaacgccat caccagtaaa aacgccagcc agaagttagg catcgacacg ccaagaaacg
    29941 cgatgaaacg tacggcgaaa tccggcagac ggtcgcgatg gcgcgccgcc cagataccga
    30001 gcggtacgga agtgagcaga attaatacca gcgccgcacc tgcaagttcc agcgtggcgg
    30061 gcaggaagtt cagcatatcg tccagcaccg ggcgttggct ggcgaatgag ataccaaagt
    30121 caagatgcag cgccttccac aaccaggtac cgtactggac gtacagcggc tgatccagcc
    30181 cgagcatggt gcgggtagag gccagcatct ccggcgtcgg cggcaggtta gataaacgca
    30241 gataatcgag cgccgggtcg ccggtaccga ggcgcagcat cagaaaaatg atcaccgagg
    30301 cggcgagcac catcgggatc agcagcagaa agcggcgtaa tacgtaacgc aacattaagg
    30361 tttcaccggt ttaatctgtt cgaacggaat ttcggtggcg atcggcgcgt aggggatatt
    30421 acccagctcc ggttttgata ccaccatcat cgagatgtag ctgataggca gataaaccgc
    30481 ctcgtcatgc agacgggtca aaatgtcgcg atacagcgcc tgacgttgcg tttcgtcatg
    30541 ggtcgccagc acttcgccga tctctttatc aatcagcggc ttgtcggcta atccttgctg
    30601 tgcctggaag tcagcgtgcg acggtacgcg cattgaactc aggaaggcgt gtggatcgta
    30661 tggcgcgccc caggtgcggt ggaaaatcat gccaaaacga ccgtcgcgct gacgagcata
    30721 gatactgctc tcttcttcgc caatcagcga gacatctgcg ccaatctggc gcatatcagc
    30781 ctgaatgatt tccgccatcg atttgcttaa cgcatcggtg ccgatgaacg acagttcaat
    30841 gcgcagcggc tgaccatttt tctcgcggat gtctttgccc gcaggcagcg tccaaccggc
    30901 tttttccagc aactctttcg ctttctgcgg atcgtactgg cgcggtttca ggccgagatt
    30961 agcgtaaggc acagaagggg caaacagggt gtcggcgacc tgctgggtgc catataacgc
    31021 gttatcaatc agcgattttt tgtttaccgc gtaattaaga gcttcacgta ctgccagctc
    31081 gttggtgggg gctttggcgg tattgagcgc cagcatcacg gtttcgatcg gctgtgacag
    31141 ttgggtgtgg taagccggat tctggctaaa gcgggcgaag gtatcgagcg gtaataaccc
    31201 ttcgtttccg tacagcaggt cgatatcgcc agtttcaaac gccaccgcgc gggtagtcgg
    31261 gtccgggatg acgttaaagg tgatcttttt aatcgctggc ttttcgcccc agtagttttc
    31321 gttacggacg aagacatcgt actgattcag tttcgattcc tgcaaaatcc acggtccggt
    31381 gccaatcggc gctttaattc cgttcatggt ttcatggttt ttaaactgcg agggagcgat
    31441 aaaacggaaa ggacggggca gggccagttc ttgcaggaaa ggatagtagg cgcttttcag
    31501 ggtaatttgc agctctgttt tactgagtgc tttaacatca acaatctggt ttgccagctc
    31561 cagccaggcg tgacgttgac ggttatcgag cactgcgcgg aagttttctg ccgccgcctc
    31621 ggcatcgaac ggttcaccgt tggagaattt cacgtcatca cgcagggtga aggtccaggt
    31681 tttaccatct tctgaatgag tccagctttt tgccagccac gggatcaccg aaccgtctgc
    31741 ctgatatttc accaatggtt cataaaccat gctctgggcg aacatctggt taggcgtgta
    31801 aaggtgcggg tttagtggcc cgacattcac cggccaggcg gtagtgattt catctggtgc
    31861 agcggcatgg acgataaaag acgcacaagc cagcagcgca aatagagtgc ggcggagtgt
    31921 ggagagcatg gtaaccccaa tggattaaaa tagatggcgg aaataagtat gacgatttta
    31981 agtattcgtc atactgatta cctgttctgg atcaatagat gggcagtcgg gtgggcggat
    32041 cagttaactg aatcgatcca ttgcacactg tcggcggtga gcgtaaaggg ggtgggggtg
    32101 cagatcgcaa ggcttaaatt ttctagctga caatggctga ctgacagcga ggagtgatag
    32161 gtgctatcta cgctgacgat ttgccaggcg ctgccgccac gctgtttaac gatggcttct
    32221 ttgcgcgtcc agatgcgcca gaacatttcc agttgctgat caggatgcac ggcgtccatt
    32281 tcagcgtgtt ccccgaggct gaacacggcg ttcgccagcc agcgccagtt ggcgcgcggg
    32341 cgaatcactt cgatatcgca gccgacttcg ccttcatcgc tcaacagcag agcgatatcg
    32401 tcaccgctat ggcttaagtt gaaccatagc ggcgtttccg gcgcgaatgc aggtttgcct
    32461 tgttcgccat agatgatctc cggtagcggg gaaagcgtgt gcgaaagcaa tgcacgcccc
    32521 gccagccagc gttcgcgtcg tggaccttgc ggtgcttgct cgcgtaaacc cggtggcagt
    32581 ggagctgcgc ttaaggtcga aactttcccc agaactatcc gatacatatc agggccaacg
    32641 tttaatggaa aatgaaagtg cgtatcgtat cacttgtcgc ctcatcccgg taaccgactt
    32701 ttcggtctgc ctggccccag taaaatcgcc agtttgctac cgcctttggt ggtttccatc
    32761 cagattttac acacactggt taatggcacc gaaagtagca tccctaccgg gccgagcagc
    32821 catccccaaa ttaacaatga aagaaatacc accatggtgg acatccccag gcgatggccc
    32881 atcatccgtg gttctaaaat attgccgatg accatatgga ccactaaaaa caatgcgctg
    32941 accagaatac attcgtaaac accattaaac agcagcacct gaatcattgg cggtacggcg
    33001 gaaattaccg cgccgatatt gggcacgtag ttgagcaaaa acgccagtac tgcccacatc
    33061 agcgcaaact gcacccccat cagctccagc cccagccaga cgatgacacc tgtccataaa
    33121 ctgagtagcg tcttcaatgc aagatagtgc gaaacgcctt taagtgcgcg gtgtaatcct
    33181 gcgatgtgaa tctgtgggtt attcagcgca aaacgcattt tgtaagggac gtggcgcact
    33241 tcaaacagca taaaaactac ggtcatcacc agcaaaagca cgctcgccat tgccccggaa
    33301 agcccggtca ttagcgctgt ggtgaaggta accacttttt ccgagtccat ccgctgcagc
    33361 attcgctccg gcgacatatg caaattaaga aaaggcaaca tctcctgcaa tttaaaaagt
    33421 ttgcgcgtca gctccttatt aaacttcggc agcatagaga taaattcgtt aaacgatgcc
    33481 gccagtacgc cgaccagcgc ggttagtgcg atcagcatca ccaccactac aatcgtaatg
    33541 gcaacggggc gttgtactcc ccgacgaata aaccaggtaa cgagcgggtt gaggacgatg
    33601 gcaaaaaaca gcgccagtaa cagctgcaca atgatatctg ccgctgcgtg aatgcccgcg
    33661 aggatcacta ccagcgaggc cagcttgagc agaatgtgca tgcccgtttt atcgggttga
    33721 gggatttcca ttggggcttc cttgtgactt tttgtattaa gtgtagcggg agtcacgcca
    33781 gcaatattct gattcttact gctgattttc acgtcagcac gtggtaaaaa tgaaacactg
    33841 ttgtaaaaat gtggtgatcc tcatgcccga acccgtagcc gaacccgcgc taaacggatt
    33901 gcgcctgaat ttgcgcattg tctccattgt catgtttaac ttcgccagct acctcaccat
    33961 cgggttgccg ctcgctgtat taccgggcta tgtccatgat gtgatgggct ttagcgcttt
    34021 ctgggcagga ttggttatca gcctgcaata tttcgccacc ttgctgagcc gtcctcatgc
    34081 cggacgttac gccgatttgc tgggccccaa aaagattgtc gtcttcggtt tatgcggctg
    34141 ctttttgagc ggtctgggat atctgacggc aggattaacc gccagtctgc ccgtcatcag
    34201 cctgttatta ctttgcctgg gacgcgtgat ccttgggatt gggcaaagtt ttgccggaac
    34261 gggatcgacc ctgtggggcg ttggcgtggt tggctcgctg catatcgggc gagtgatttc
    34321 gtggaacggc attgtcaatt acggggcgat ggcgatgggt gcgccgttag gcgtcgtgtt
    34381 ttatcactgg ggcggcttgc aggcgttagc gttaatcatt atgggcgtgg cgctggtggc
    34441 cattttgttg gcgatcccgc gtccgacggt aaaagccagt aaaggcaaac cgctgccgtt
    34501 tcgcgcggtg cttgggcgcg tctggctgta cggtatggca ctggcactgg cttctgccgg
    34561 atttggcgtc atcgccacct ttatcacgct gttttatgac gctaaaggtt gggacggtgc
    34621 ggctttcgcg ctgacgctgt ttagctgtgc gtttgtcggt acgcgtttgt tattccctaa
    34681 cggcattaac cgtatcggcg gcttaaacgt ggcgatgatt tgctttagcg ttgagataat
    34741 cggcctgcta ctggttggcg tggcgactat gccgtggatg gcgaaagtcg gcgtcttact
    34801 ggcgggggca gggttttcgc tggtgttccc ggcattgggc gtagtggcgg taaaagcggt
    34861 tccgcagcaa aatcaggggg cggcgctggc aacttacacc gtatttatgg atttatcgct
    34921 tggcgtgacc ggaccactgg ctgggctggt gatgagctgg gcgggcgtac cggtgattta
    34981 tctggcggcg gcgggactgg tcgcaatcgc gttattactg acgtggcgat taaaaaaacg
    35041 gcctccggaa cacgtccctg aggccgcctc atcatcttaa aacttactga ataaccagcg
    35101 tattaatgat gttttctgcg gtggtctgcg ctttttgctg atcgtcagct ggcagagtaa
    35161 tttgcatggt cagcagttga ttacccacgt tacccagaat aacggaagag tacgccgtct
    35221 ggcctttcgc ggagataata ctgtctaact gctgcatttt gtgacctttc agctcaatgg
    35281 ctttgttggt taccacttgc agctgcggat cgcggctacg ttgctgatct tccagacgct
    35341 tcgccaacac cgccagatct tctttcggat catcgcccat gatgacgatg actgctttct
    35401 gcccggtggc gtcggaccag acatgcatgt tattggcctg cgttcccagc ttaccgctct
    35461 ggtcggtcat atccgctggc agcgagaaac ttaacttgcc atcaagcagg ttgacgggat
    35521 tcccggtagc gttactttcc gcgaccgaac cctgcgccgt agcgttagtg tctttatcat
    35581 cacaggccgc aagccccata accagcaggc caattccgac atatttaacc agattgcgca
    35641 ttgacttctt cctttcgata aacggccata acggctcatt catccatctt atcacaactc
    35701 tgataacgaa cctttaactc gcctgcaaag cgttgatttc ggatttatct gccagtcttt
    35761 tcaacagaat attgagtaat acgccataca ttggcaggaa gaaaacgata ctgattaaca
    35821 ctttgaaaca gtaatcgacc agcgcgattt ccatccagtg ttcagccata aaggcatccg
    35881 ggctacgcca gaaggcaatg aagaaaaagg ccagcgtgtc gctgacgtta ccgaacagtg
    35941 tggacgctgt cggtgccagc caccagcggc gactctgacg caggcggtta aaaacgtgca
    36001 cgtcgaggat ctgtcccagc gcgtaggcca tgaaactggc ggtggcgata cgggcgacaa
    36061 acaggttgaa gtgggcgagt gcgccgaatc cctgccagga acccatatag aatagcgacg
    36121 agatgacgta ggagatcagc aacgccggga tcatcaccgc aaagatgata cgacgggcca
    36181 gcggtgcgcc aaaaatacgc acggtcaggt cggtagcaag gaaaataaac gggaagctaa
    36241 acgcgcccca ggtggtatgg aaacccaaaa tggagacggg aagctgaacc agatagttac
    36301 tggaggtgat caccagcaga tgaaataacg ataaccagaa caacgcctta tagcgttgag
    36361 tttgcgagaa aacgttcata ttgtaccttt ttgattaacc attggggtga gggaacccaa
    36421 tacgtacgac acgtctgtta ccttaacata ataacgtgct tacccttttt tcgagccgcc
    36481 gcatgatact gctttacgtt gacaatgcaa tggttaattt tcacgcaatc gttaacctgg
    36541 tttgcttacg gactcacagg gcgtaaacta gcgccgtttt tttatgtgat gagaagaaaa
    36601 tgaccgatct cttttccagc cctgaccaca cactcgacgc acttggcctg cgctgcccgg
    36661 aacccgtaat gatggtgcgc aaaactgtgc gcaatatgca gcctggcgaa acgttgctga
    36721 ttatcgccga cgatccggcc actacccgcg atattcctgg gttttgtacc tttatggaac
    36781 acgaactggt tgcgaaagag acggatggac tgccttatcg ttatttgatt cgtaaaggcg
    36841 gttgatgggg aaagattggc ttcgatgccg ccttttcccc tcaccctaac cctctcccca
    36901 gaggggcgag gggaccgatc gcgctcaatg ttgcgatcgg tttgccttat ctcctgcgca
    36961 acaatcttaa cgcattcgct gtcaccagca ccgtcgcacc cgtatctgcc agcaccgcca
    37021 gccacaggcc ggtcatccct aacagcgtgg tgacgaggaa gatccctttc agccccagcg
    37081 caatggtgat gttctggcgg atattggcgt gagtggcgcg tgccagttca atcatttgca
    37141 ccaggccgcg caggtggtta tgggttaatg ctgcatcagc ggtttccagc gccacgtctg
    37201 tgccactgcc cattgcaatc ccaatggcgg cagctttcat cgctggcgcg tcgttaatac
    37261 catcaccgac catcgccagg ggcgcatgtt gattcagctt ggtcaccgct ttgactttat
    37321 cttccggcaa taggcccgct ttaaactcca gccccagctc cccggcgatt gccgccgctg
    37381 cacgtggatt atcgccggtg aggatcactc ctttgacccc cagcgcgttc agttcactga
    37441 tggcagttgc agcatcggcg cgcagggtat cctgcaatgc aatgataccc agcacgtcat
    37501 cattacgtac taccagcaca accgtttgcc cggcgctttc cagttcatta atcaaaccag
    37561 caaatgcatc agcgggatgt ttcccggcag cgcaaatcaa tacgcgctca ccgttaacct
    37621 gcgcttcaat gccagacccg accagcgccc gctgtgattc ggcggtggga atggcgagtt
    37681 cagcaacctg tgcttcgcgt acgatggctt gcgccagtgg atgcgtcgcg ccttgctcga
    37741 ccgccgccgc cagtgtcagc agttcagatt cactaatacc cgttgccgga tgaatcgcgg
    37801 taacgcgcgg tttaccgacg gtcagcgtac cggttttatc aaacgccacc tgagtaacac
    37861 gacctagttg ttccagcgcc gctccgcctt taatcaacgc cccacgacgc gctgccgccg
    37921 ccagcccgga ggtaatcgcc gcaggcgttg agataactaa cgcacacggg cagccaatca
    37981 gcagcagcgt cagcccttta taaatccact cctgccagct ggcggcaaac agcagcggtg
    38041 gcaccagcgt caccagcaga gcgacggcca taatcgcggg cgtatagata cggctgaaac
    38101 ggtcgataaa ccgctcaatg ggggcgcgac gctcttcggc ttcttcgatc agtttcagaa
    38161 tccggtcaat ggcgctggca cccggttctg acagcacttc cagcgttacc agacggtcta
    38221 cgctggtggc tcctgcagga actttatcgc ccgtcgcgcg ctccaccgga atggattctc
    38281 cggtcagggc gctttcatca aaactggcaa acggtgagag cagtttaccg tcggcaggca
    38341 aacgcccacc tgcggcgact tcaatcacat cgccaggccg caggctgtta atcgccacct
    38401 cttcccgctc accgttacgc aggcgcgtgg cggtttctgg tttcagcgcc attaacgcgc
    38461 taacgccctg acgcgcgcgg ctggcggccc agccttccag tcgttcacca atcaaaaaca
    38521 gcagcaacac catcgcagct tcagccgttg cgccaataaa cagtgcacca atagcggcta
    38581 cgctcattaa ggtttcaatg gcgaagtagc tgccggattt gatcagccgt aatgcctgac
    38641 gagcaatcgg gtacagccca accagcgtgg tcgcgataaa cgccagttgc ccgaacggat
    38701 gattgaactg ctccagaccc cagctgattg ccatcatcac gattagtgta atcagcggca
    38761 gattctcttt caggcgcgat gcttgtggct cgtcggcggc ctgctcatcg cgcagggaat
    38821 agcccgcttt ttgcaccgca gattcaactt gtgcacgaat gtcattgtcg gcatcaacca
    38881 ccagtttttc ggtggcgaac aacacctgca cctgattcac acctgcaagc tggcgcacgg
    38941 cattttctac cttgcgcgca caggcggcgc agtccatgcc actgactttc cagctatagc
    39001 gggtgccgga gacgttttca gagagcgttg gcgagctgga gcatgcgccg tcgcagcaac
    39061 agtcgttggc gttctgtacc gtggttagcg gtttgaacgc agcaaattga ggggctttct
    39121 tgccgtgatt gtcaggtgtc gacatggcat actccggtta agttttttct cattaaccga
    39181 aggatacact ctggagtcga ctccagagtc aagttttatc agagatacag tgagcggacg
    39241 atgaggaagt gaccggcaaa gtagcaggcc gcagcaatcg cgttatccgc acggaagcgt
    39301 cggcgatagt ggctccccag ccagacaaag ttactgataa acagcaacga agcgccaaca
    39361 aacgcagaga gcgccggagc ggtcggacgg aagaaccaca gttcacctgc cagccacacc
    39421 atcaccagcg tcatgccgat aaacgtgcag ataggccaac gatactcttc cagccgcgtc
    39481 cagataatcg ccagtaacag cgcacccaga accagcaaca ccagtggcag aggccagaag
    39541 aaagagagcg tcatctgact ggcgaaatag atggtgtaca gcaggtgcga gaggaaaaac
    39601 gcgccgatgg cgtacatcag acgttgacgt ggcaacaggg ttagcgcatc gcccagcagt
    39661 gaggcgcaca gccctgccag caccagatag ctaatggcgt cgaacatcgg cgcttgccag
    39721 gccagtaaca gcaggagaag aagggttaac ggtttaaaca cccagcgttg ccaggttggc
    39781 ccacgatacg atgcatccac agatagccat gcggaaagac agacagcgat aaacgaccaa
    39841 agcatcttag ttccttgaat tcgttatttt ttcgtaagcg gaatgcctac ggtcttgctt
    39901 ccagtttagt ggcgagggtg gacggatgac aacaccataa ctggcagggt atgcttattt
    39961 ccgcattttc cgatgaggga taaagatgag caaaccacct cttttcttta ttgttatcat
    40021 tggcttaatt gtcgtcgccg catcgtttcg ttttatgcaa cagcgacggg aaaaagctga
    40081 taatgatatg gctccgctcc agcaaaagct ggtggtggtg agcaacaagc gggaaaaacc
    40141 gattaacgat cgccgttcgc gccagcagga agtgactccg gcaggcacca gtatgcgcta
    40201 cgaggcaagc ttcaaaccgc aaagcggagg aatggagcag acgtttcgcc tcgacgccca
    40261 gcagtaccac gccctgacag tgggcgataa aggtacgctg agctataaag gaacgcgctt
    40321 tgttagcttt gttggcgaac aataaggtta ttttttaact ttaaatttct tctgccagac
    40381 cagcagttca aacacgccga aaaggaaaat ccgtaccttt tcggcggtgg tcatctgcgg
    40441 gccatctttc ggtaaagtgg atttcaatag cgccagctgc ataccatgca tcagcacggt
    40501 aaaaatcagc gccacgttaa cgaaaatatt tagcgggcgc gggaagggat gcaccagatt
    40561 gaggattaaa aatccccaaa cgcagagcat taacaaacga ccaatattaa tcagcatcac
    40621 tttctccttg tgcttcgcgt tgatacagcc gataagccac ctgacccgcc actttttccc
    40681 gatgtaacga ccagtttgcc ggaacggtgg gcagaccgtt ttcgacttcg ctttcgacat
    40741 aaatcagggc ttcgtcagcc agccagccgt tatcttccag taaatttatc gtctcttcta
    40801 acaagccacg gcggaacggt ggatcgacaa acacgatatt atgcggtgtg cctttttgcg
    40861 ccaggaatga catcgcgttg ctgttcacca cgcgtgcatt gcctgctttt agtgtcgcca
    40921 gattcttaat taactgctga gaaactgcac gatccatctc aattaacgtg gcccccgcag
    40981 cgtagcgcga taacgcttcc agccccagcg cgccgctccc ggcgaagcaa tccagacatt
    41041 gggcgtcaac aatgaccgga gccagccagt taaacaacgt ttcgcgtacg cggtcggtgg
    41101 tggggcgcag acctgggcta tcaggaaccg ggagtttacg gcctcgccac tgcccgccaa
    41161 taatgcggat ttggccgctg ccggaatgat tcggtttttt catgataaat tgctcaatcc
    41221 gccagataac acatacttgc aggcggtgat gtgaattaag ttaagtgata gactatttca
    41281 tcattttttt agctgctatg tacatagcgt taacgctgtg ccatgaagca acagcgagga
    41341 gtgtagtcgc aaatggcgaa agaaaaaaaa cgtggctttt tttcctggct gggctttggt
    41401 caaaaagagc agaccccgga aaaagagaca gaagttcaga atgaacaacc ggttgtagaa
    41461 gaaatcgttc aggcgcaaga gcctgtgaag gcttcggaac aagccgttga agagcagccg
    41521 caggcgcata ctgaagccga ggcggaaact tttgctgccg acgttgtgga agtcactgaa
    41581 caggttgttg aaagtgaaaa agcgcagcct gaagcggaag ttgttgcaca gccggaaccg
    41641 gtcgtagaag aaacgccgga gccagtggct atcgaacgtg aagagctgcc gttgccggaa
    41701 gacgtcaacg ccgaagaggt ttcgccagaa gagtggcagg ctgaagcgga aaccgtagag
    41761 attgtcaaag cggcggaaga agaagcggct aaagaagaaa tcaccgacga agagctggaa
    41821 gcacaggcgc tggcagccga agcagcagaa gaggcggtga tggtggttcc tccggcagaa
    41881 gaagagcagc cggtggaaga aatcgctcag gagcaggaaa aaccaaccaa agaaggtttc
    41941 ttcgcgcgcc tgaaacgcag cctgttaaaa accaaagaaa atctcggttc cggatttatc
    42001 agcctgttcc gcggtaaaaa aatcgacgat gatctgtttg aggagctgga agagcagctt
    42061 ttgatcgccg atgtgggcgt ggaaaccaca cgtaaaatta tcaccaatct gacggaaggc
    42121 gcatcccgca agcagcttcg tgatgccgag gcgctctatg gcctgctgaa agaagagatg
    42181 ggtgagattc tggcgaaagt cgatgagccg ctgaatgttg aaggtaaaac gccgttcgtg
    42241 atcctgatgg tgggcgtcaa cggtgtgggt aaaaccacga cgattggtaa gctggcacgt
    42301 cagtttgagc agcagggtaa atcggtgatg ctggcggcgg gcgatacttt ccgtgcagca
    42361 gcggttgaac agcttcaggt ctggggtcag cgcaacaata ttccggtgat tgcccagcat
    42421 accggggcgg attccgcctc tgttatcttc gacgccattc aggcagctaa agcgcgtaat
    42481 atcgacgtcc tgattgccga taccgccgga cgcctgcaga acaaatcgca cctgatggaa
    42541 gagttgaaga aaatcgtccg cgtgatgaag aaactcgacg ttgaagcgcc gcatgaagtt
    42601 atgctgacta ttgatgccag caccgggcag aacgcggtaa gccaggccaa actgttccac
    42661 gaagctgttg gcttaaccgg catcacgcta acgaaactgg acggcacggc gaaaggcggg
    42721 gtaattttct cggtggctga ccagtttggt atccctatcc gctacattgg tgtcggcgaa
    42781 cgtattgagg atttgcgtcc gtttaaggcg gacgacttta tagaggcact ttttgcccga
    42841 gaggattaac aatgattcgc tttgaacatg tcagcaaggc ttatctcggt gggagacagg
    42901 cgctgcaggg cgttacgttc catatgcagc cgggtgagat ggcgtttctg accggtcatt
    42961 ccggcgcagg gaaaagtacc ctcctgaagc tgatctgtgg gattgagcgg cccagcgccg
    43021 ggaaaatctg gtttagcggc catgacatca cgcgtctgaa aaaccgtgaa gttccgtttc
    43081 tgcgtcgcca gattggcatg attttccagg atcaccatct actgatggac cgtactgtct
    43141 acgataacgt ggcgatcccg ctgattatcg ctggtgccag cggtgacgat attcgtcgcc
    43201 gggtgtcggc ggcactggat aaagtcgggc tactggacaa agcgaagaac ttccctattc
    43261 agctttccgg cggtgaacaa cagcgtgttg gcattgcccg cgcggtggtg aataagcccg
    43321 cggtactgct ggcggacgaa ccgaccggta acctggacga cgcgttgtcg gaagggattc
    43381 tacgtctgtt tgaagagttt aaccgcgttg gggtaaccgt attgatggca acgcacgaca
    43441 tcaacctgat ttcacggcgt tcccatcgca tgctcaccct gagcgatggt cacttgcatg
    43501 gaggcgtggg ccatgaataa gcgcgatgca atcaatcata ttcggcagtt tggtgggcgt
    43561 ctggatcgct tccgtaaatc ggtcggcggc tcaggcgacg gcggtcgtaa cgcaccgaaa
    43621 cgcgcgaaat cctcgccaaa accggtaaat cgcaaaacca acgttttcaa cgaacaggtg
    43681 cgctatgcct tccacggcgc attgcaggat ctgaaaagca aaccgttcgc cacgttttta
    43741 acggtgatgg ttatcgccat ctccctgacg ctgcccagcg tctgttatat ggtgtacaaa
    43801 aacgttaatc aggcggcgac gcagtattat ccgtcaccgc aaatcactgt ttacttgcaa
    43861 aaaacgctgg acgatgacgc cgctgcgggc gtggtggcac agttgcaggc cgagcaaggc
    43921 gtggagaaag tgaactatct ttctcgtgaa gacgcactgg gcgagttccg taactggtct
    43981 ggttttggtg gtgcgctgga tatgctggaa gaaaacccgc ttccggcagt ggcggtggtg
    44041 atcccgaaac tcgattttca ggggacggaa tcactcaata cgctgcgtga tcgtatcacg
    44101 cagattaacg gcattgacga agtgcggatg gatgacagct ggtttgcccg tctggcggcg
    44161 ttgaccgggc tggtcgggcg cgtttcggcg atgatcggcg tgttgatggt ggcggcagtg
    44221 ttcctcgtca tcggcaacag cgtacgtctg agcatctttg ctcgccgtga ctccatcaac
    44281 gtgcagaaac tgattggtgc gacggatggt ttcatccttc gcccgttcct gtatggtggc
    44341 gcactgctgg gattttctgg cgcattgttg tcattaattt tgtcagaaat tctggtgctg
    44401 cgattgtcat cggcggttgc ggaagtggca caggttttcg gaacgaagtt tgatatcaat
    44461 ggtttatcgt tcgatgaatg cctgctattg ctgctggtat gctcgatgat tggctgggtg
    44521 gcagcgtggc ttgccacggt acaacattta cgccacttta cgcctgaata ataaaagcgt
    44581 gttatactct ttccctgcaa tgggttccgt agcagggaaa gagatcccgt tgtctcttcc
    44641 cggtatttca tctctatgtc acattttgtg cgtaatttat tcacaagctt gcattgaact
    44701 tgtggataaa atcactgtct gataaaacag tggatgataa cctcgttgct cttaagctct
    44761 ggcacagttg ttgctaccac tgaagcgcca gaagatatcg attgagagga tttgaatgac
    44821 tgacaaaatg caaagtttag ctttagcccc agttggcaac ctggattcct acatccgggc
    44881 agctaacgcg tggccgatgt tgtcggctga cgaggagcgg gcgctggctg aaaagctgca
    44941 ttaccatggc gatctggaag cagctaaaac gctgatcctg tctcacctgc ggtttgttgt
    45001 tcatattgct cgtaattatg cgggctatgg cctgccacag gcggatttga ttcaggaagg
    45061 taacatcggc ctgatgaaag cagtgcgccg tttcaacccg gaagtgggtg tgcgcctggt
    45121 ctccttcgcc gttcactgga tcaaagcaga gatccacgaa tacgttctgc gtaactggcg
    45181 tatcgtcaaa gttgcgacca ccaaagcgca gcgcaaactg ttcttcaacc tgcgtaaaac
    45241 caagcagcgt ctgggctggt ttaaccagga tgaagtcgaa atggtggccc gtgaactggg
    45301 cgtaaccagc aaagacgttc gtgagatgga atcacgtatg gcggcacagg acatgacctt
    45361 tgacctgtct tccgacgacg attccgacag ccagccgatg gcaccggtgc tctatctgca
    45421 ggataaatca tctaactttg ccgacggcat cgaagatgat aactgggaag agcaggcggc
    45481 aaaccgtctg accgacgcga tgcaaggtct ggacgaacgc agccaggaca tcatccgcgc
    45541 gcgctggctg gacgaagaca acaagtccac gttgcaggaa ctggctgacc gttacggtgt
    45601 ttccgctgaa cgtgtacgcc agctggaaaa gaacgcgatg aaaaaactgc gcgctgctat
    45661 agaagcgtaa tttccgctat taagcagaga accctggatg cgagtccggg gtttttgttt
    45721 tttgggcctc tacaataatc aatttcccct ccggcaaaac gccaatcccc acgcagattg
    45781 ttaataaact gtcaaaatag ctattccaat atcataaaaa tcgggtatgt tttagcagag
    45841 tatgctgcta aagcacgggt agccatgcat aaaacgaaat aaagtgctga acaacaacat
    45901 cacaacacac gtaataacca gaagaatggg gattctcagg atgaacataa agggtaaagc
    45961 gttactggca ggatgtatcg cgctggcatt cagcaatatg gctctggcag aagatattaa
    46021 agtcgcggtc gtgggcgcaa tgtccggtcc ggttgcgcag tacggtgacc aagagtttac
    46081 cggcgcagag caggcggttg cggatatcaa cgctaaaggc ggcattaaag gcaacaaact
    46141 gcaaatcgta aaatatgacg atgcctgtga cccgaaacag gcggttgcgg tggcgaacaa
    46201 agtcgttaac gacggcatta aatatgtgat tggtcacctc tgttcttcat caacgcagcc
    46261 tgcgtctgac atctacgaag acgaaggcat tttaatgatc accccagcgg caaccgcgcc
    46321 ggagctgacc gcccgtggct atcagctgat cctgcgcacc accggcctgg actccgacca
    46381 ggggccgacg gcggcgaaat atattcttga gaaagtgaaa ccgcagcgta ttgctatcgt
    46441 tcacgacaaa cagcaatacg gcgaaggtct ggcacgagcg gtgcaggacg gcctgaagaa
    46501 aggcaatgct aacgtggtgt tctttgatgg tatcaccgcc ggggaaaaag atttctctac
    46561 gctggtggcg cgtctgaaga aagagaatat cgacttcgtt tactacggcg gttaccaccc
    46621 ggaaatgggg caaatcctgc gtcaggcacg cgcggcaggg ctgaaaactc agtttatggg
    46681 gccggaaggt gtggctaacg tttcgctgtc taacattgcg ggcgaatcag cggaagggct
    46741 gctggtgacc aagccgaaga actacgatca ggttccggca aacaaaccca ttgttgacgc
    46801 gatcaaagcg aaaaaacagg acccaagcgg cgcattcgtt tggaccacct acgccgcgct
    46861 gcaatctttg caggcgggcc tgaatcagtc tgacgatccg gctgaaatcg ccaaatacct
    46921 gaaagcgaac tccgtggata ccgtaatggg accgctgacg tgggatgaga aaggcgatct
    46981 gaaaggcttt gagttcggcg tatttgactg gcacgccaac ggcacggcga cagatgcgaa
    47041 gtaatcatta atcggcaact ttgggttgcc gccaaattgc taatatcgag tacgttgctt
    47101 catgccggat gcggcgtaaa cgccttatcc ggcctataag atccaaagaa atcagtaaat
    47161 tgcaacacac attgtaggcc tgataagcgt agcgcatcag gcaatttctc gttggtcatc
    47221 acttttgaaa tcaacttgac gattaacgct tctcccagcc gccctgttgc gccgtaaatc
    47281 ccagtgcctg cataaacgct gtcatcacac cgcgatcttc cacgcctgca tccgccatcc
    47341 accagcatga aacgccagga ttgttacgca aaacctcttc cagcagatat tgccccacac
    47401 cgcgacgtcg ggtgacttcc cgcacacgca gggaatccag tgctccctcg gtgccgctta
    47461 aggttacccg tacggcagcg agcaggcgct cgttaaaacg cgcggcgtag atacggtggt
    47521 tatcgtcaac ctgtaaagag gaaggggaat actccggcca gatcttttgc aggtcaatcc
    47581 ggtcctggtc gctaaagttt tctaatcgaa tgatggtcag cttcatgggt aacccgtgta
    47641 aatcacaaaa gtggaaccag tgtagcgaaa taatttaatc ggaggctttc tcttttttat
    47701 ttcttttggc aggtgattaa ttttttaaca gcaataatta caaagttaaa acattagaga
    47761 atgaaaaata cccagcataa tcccctgaat ggtagtgaat tattccgccc tttgtgccgt
    47821 tattttatgc tgacaaaggc acttttttct gtttatctat caataaattc agaatattat
    47881 ctgttcttaa tcgactgaaa aatagggatt ttaatcacta ttatcacaaa atactgcgct
    47941 aaacccttaa tcagacaggc aaaaacagtg cagtataaaa aaagaacagt ctgatttgtt
    48001 aacacataaa aacaaagcaa cacaacatca cgaatgggga tttttgacta tgaaacggaa
    48061 tgcgaaaact atcatcgcag ggatgattgc actggcaatt tcacacaccg ctatggctga
    48121 cgatattaaa gtcgccgttg tcggcgcgat gtccggcccg attgcccagt ggggcgatat
    48181 ggaatttaac ggcgcgcgtc aggcgattaa agacattaat gccaaagggg gaattaaggg
    48241 cgacaagctg gttggcgtgg aatatgacga cgcctgcgac ccgaaacaag ccgttgcggt
    48301 cgccaacaaa atcgttaatg acggcattaa atacgttatt ggtcatctgt gttcttcttc
    48361 tacccaacct gcatcagata tctacgaaga cgaaggtatt ctgatgatct cgccgggagc
    48421 gaccaacccg gagctgaccc aacgcggtta tcaacacatt atgcgtactg ccgggctgga
    48481 ctcttcccag gggccaacgg cggcaaaata cattcttgag acggtgaagc cccagcgcat
    48541 cgccatcatt cacgacaaac aacagtatgg cgaagggctg gcgcgttcgg tgcaggacgg
    48601 gctgaaagcg gctaacgcca acgtcgtctt cttcgacggt attaccgccg gggagaaaga
    48661 tttctccgcg ctgatcgccc gcctgaaaaa agaaaacatc gacttcgttt actacggcgg
    48721 ttactacccg gaaatggggc agatgctgcg ccaggcccgt tccgttggcc tgaaaactca
    48781 gtttatgggg ccggaaggtg tgggtaacgc atcattgtcg aatattgctg gcgatgctgc
    48841 cgaaggcatg ttggtcacta tgccaaaacg ctatgaccag gatccggcaa accagggcat
    48901 cgttgatgcg ctgaaagcag acaagaaaga tccgtccggg ccttatgtct ggatcaccta
    48961 cgcggcggtg caatctctgg cgactgccct tgagcgtact ggcagcgatg agccgctggc
    49021 gctggtgaaa gatttaaaag ctaacggtgc aaacaccgtg attgggccgc tgaactggga
    49081 tgaaaaaggc gatcttaagg gatttgattt tggtgtcttc cagtggcacg ccgatggttc
    49141 atccacggca gccaagtgat catcccaccg cccgtgaaat gcgggcgggt ttagaaaggt
    49201 taccttatgt ctgagcagtt tttatatttc ttgcagcaga tgtttaacgg cgtcacgctg
    49261 ggcagtacct acgcgctgat agccatcggc tacaccatgg tttacggcat tatcggcatg
    49321 atcaacttcg cccacggcga ggtttacatg attggtagct atgtctcctt tatgatcatc
    49381 gccgcactga tgatgatggg cattgatacc ggctggctgc tggtggcggc gggattcgtc
    49441 ggcgcaatcg tcattgccag cgcctacggc tggagtatcg aacgggtagc ctatcgcccg
    49501 gtgcgtaact ctaagcgcct gattgcgctc atctcggcaa tcggcatgtc catcttcctg
    49561 caaaactacg ttagcctgac cgaaggttcg cgtgacgtgg cgctgcccag tctgtttaac
    49621 ggtcagtggg tggtggggca tagcgaaaac ttctctgcct ctattaccac catgcaggca
    49681 gtgatctgga ttgttacttt cctcgccatg ctggcgctga cgattttcat tcgttattcc
    49741 cgtatggggc gcgcctgccg tgcctgcgcg gaagatctga aaatggcgag cctgcttggc
    49801 atcaacaccg accgggtgat tgctttgacc tttgttattg gcgcggcgat ggcggcagtg
    49861 gctggcgtac tgctcggtca gttctacggc gtcattaacc cctatatcgg ctttatggcc
    49921 gggatgaaag cctttaccgc tgcggtgctc ggtgggattg gcagcattcc aggggcgatg
    49981 attggcgggc tgatcctggg gattgcggag gcgctctctt ctgcctatct gagtacggaa
    50041 tataaagatg tggtctcatt cgccctgctg attctggtgc tgctggtgat gccgaccggt
    50101 attctgggtc gcccggaggt agagaaagta tgaaaccgat gcatattgca atggcactgc
    50161 tctctgccgc gatgttcttt gtcctcgctg gcgtctttat gggcgtgcaa ctggagctgg
    50221 atggcaccaa actggtggtc gacagcgctt cggatgtccg ttggcagtgg gtgtttatcg
    50281 gcacggcggt ggtctttttc ttccagcttt tgcgacctgc tttccagaaa gggctaaaaa
    50341 gcgtttccgg gccgaagttt attctgcccg cgattgatgg ctccacggtg aagcagaaac
    50401 tgttcctcgt ggcgctgctg gtgcttgcgg tgtcgtggcc gtttatggtg tcacgtggga
    50461 cggtggatat cgccactctg accatgatct acattatcct cggtctcggg ctgaatgtgg
    50521 ttgttggtct ttctggtctg ctggtgctgg ggtacggcgg tttttacgcc atcggcgctt
    50581 acacttttgc gctgctcaat cactattacg gtctggggtt ctggacctgt ttgccgattg
    50641 ccggactgat ggcagcggcg gcgggcttcc tgcttggttt tccggtgttg cgtctgcgcg
    50701 gtgattatct ggcgatcgtt accctcggtt tcggcgaaat tgtgcgcata ttgctgctca
    50761 ataacaccga aattaccggc ggcccgaacg gtatcagtca gatcccgaaa ccgactttct
    50821 tcggccttga gttcagccgt accgcccgtg aaggcggttg ggacacgttc agtaatttct
    50881 ttggcctgaa atacgatccc tccgatcgcg tcatcttcct gtatctggtg gcgttgctgc
    50941 tggtggtgct aagcctgttt gtcattaacc gcctgctgcg gatgccgctg gggcgtgcgt
    51001 gggaagcgtt gcgtgaagat gagatcgcct gccgttcgct gggcttaagc ccgcgtcgta
    51061 tcaagctgac tgcctttacc atcagtgccg cgtttgccgg ttttgccgga acgctgtttg
    51121 ctgcgcgcca gggctttgtc agcccggaat ccttcacctt tgctgaatcg gcgtttgtac
    51181 tggcgatagt ggtgctcggc ggtatgggat cgcagtttgc ggtgattctg gcggcaattt
    51241 tgctggtggt atcgcgcgaa ttgatgcgtg atttcaacga atacagcatg ttaatgctcg
    51301 gtggtttgat ggtgctgatg atgatctggc gtccgcaggg cttgctgccc atgacgcgcc
    51361 cgcaactgaa gctgaaaaac ggcgcagcga aaggagagca ggcatgagtc agccattatt
    51421 atctgtgaac ggcctgatga tgcgcttcgg cggcctgctg gcggtgaaca acgtcaatct
    51481 tgaactgtac ccgcaggaga tcgtctcgtt aatcggccct aacggtgccg gaaaaaccac
    51541 ggtttttaac tgtctgaccg gattctacaa acccaccggc ggcaccattt tactgcgcaa
    51601 tcagcacctg gaaggtttac cggggcagca aattgcccgc atgggcgtgg tgcgcacctt
    51661 ccagcatgtg cgtctgttcc gtgaaatgac ggtaattgaa aacctgctgg tggcgcagca
    51721 tcaacaactg aaaaccgggc tgttctctgg cctgttgaaa acgccttctt tccgtcgcgc
    51781 ccagagcgaa gcgctcgacc gcgccgccac ctggcttgag cgcattggtt tgctggaaca
    51841 cgccaaccgt caggcgagta acctggccta tggtgaccaa cgccgtctgg agattgcccg
    51901 ctgcatggtg acgcagccgg agattttaat gctcgacgaa ccggcggcag gtcttaaccc
    51961 aaaagaaacc aaagagctgg atgagctgat tgccgaactg cgcaatcatc acaacaccac
    52021 tatcttgttg attgaacacg atatgaagct ggtgatgggg atttcagacc gaatttatgt
    52081 ggtcaatcag gggacgccac tggcaaacgg tacgccggag caaatccgta ataacccgga
    52141 cgtgatccgt gcctatttag gtgaggcata agatggaaaa agtcatgttg tcctttgaca
    52201 aagtcagcgc ccactacggc aaaatccagg cgctgcatga ggtgagcctg catatcaatc
    52261 agggcgagat tgtcaccctc attggcgcga acggggcggg gaaaaccacc ttgctcggca
    52321 cgttatgcgg cgatccgcgc gctaccagcg ggcgaattgt gtttgatgat aaagacatta
    52381 ccgactggca gacggcgaaa atcatgcgcg aagcggtagc gattgtcccg gaagggcgtc
    52441 gcgtcttctc gcggatgacg gtggaagaga acctggcgat gggcggcttt tttgccgaac
    52501 gcgaccagtt ccaggagcgc ataaagtggg tgtatgaact gttcccacgt ctgcatgagc
    52561 gccgtgttca gcgggcaggc accatgtccg gcggtgagca acaaatgctg gcgattggtc
    52621 gcgcgctgat gagcaacccg cgtttgctgc tacttgatga gccatcgctt ggtcttgcgc
    52681 cgattatcat ccagcaaatt ttcgacacca tcgagcagct gcgcgagcag gggatgacta
    52741 tcttcctcgt cgagcagaac gccaaccagg cgctaaagct ggcggatcgc ggctacgtgc
    52801 tggaaaacgg ccatgtggtg ctctccgata ctggtgatgc actactggcg aatgaagcgg
    52861 tgagaagtgc gtatttaggc gggtaataac acgttgattg ataaggagtc aaaagactcc
    52921 tttgagacag gtgacaaatg taaaattgcc tgatgcgctg cgcttatcag gcctactggg
    52981 tgagttgcaa tatattgaat ttgcacgatc ttgtaggccg gataaggcgt ttacgccgca
    53041 tccggcatga aacgatgagt aatctgtaga gtttgattca gaccttctat ttttccgctt
    53101 atccgtgccc catctcccat tttccctcac ccacgccgtc accgccttgt catctttctg
    53161 acaccttact tgcaggttta tgaagttggc accgccacca tgatggcatc gaaagccatt
    53221 aaaccggtat atgacgtgtt taaagaggca gggattcaat tcgatgagtc gcagtttgtg
    53281 ccgacggttt ccggttacta ctccgacagc aaaacgggcc acttactctc ccagccattc
    53341 aacagctcga ctcccgttct ctattacaac aaagacgcct tcaagaaagc ggggttagac
    53401 ccggaacagc cgccgaaaac ctggcaggat ctggcggact cttccgcaaa actgaaagcc
    53461 tccggtatga agtgcggcta cgccagcggc tggcagggct ggatccaact ggaaaacttt
    53521 agcgcctgga acggtctgcc gtttgccagc aaaaacaacg gctttgacgg cacggacgcg
    53581 gtgctggagt tcaacaagcc ggagcaggtg aaacacattg ccatgctcga agagatgaac
    53641 aagaagggcg acttcagcta cgtcggtcgt aaggatgaat ccaccgagaa gttctataac
    53701 ggtgattgcg cgatgaccac cgcctcttcc ggttctctcg ctaatattcg tgagtacgcc
    53761 aaatttaact atggcgtagg catgatgcct tacgacgccg atgcgaaaga tgcaccgcaa
    53821 aacgccatta tcggcggggc cagcctgtgg gtgatgcagg gcaaaaataa agaaacgtat
    53881 accggcgtgg cgaagttcct cgacttcctc gcgaagccag aaaacgctgc cgagtggcat
    53941 cagaaaaccg gttatctgcc aatcaccaaa gcagcgtatg acctgacccg tgagcagggc
    54001 ttttatgaga aaaacccagg ggcggatacc gcgacgcgtc agatgctgaa taagccgccg
    54061 ttgccgttca ccaaagggct gcgtctgggc aacatgccgc agatccgcgt gattgtggat
    54121 gaagagctgg agagcgtgtg gaccggtaag aagacaccac agcaggcact ggataccgcc
    54181 gttgagcgtg gaaatcagtt gctgcgccgc tttgagaaat cgacgaagtc ttaatcagtg
    54241 taatgtcgga tgcgtttcgc ttatctgacc tggcatcgcg tgtaggccgg ataagcgaag
    54301 cgcatccggc acagttcagg aattaaccgt aatgtcatca tcccgtccgg tgttccgctc
    54361 gcgctggctg ccttatctgc tggtcgcgcc gcagctcatc atcaccgtta tcttttttat
    54421 ctggcctgcg ggcgaagcgt tgtggtactc gcggcaaagc gtcgatccgt ttggtttctc
    54481 cagccagttt gtcggtctgg ataacttcgt cacgctgttc catgacagct actatctaga
    54541 ctccttctgg acgacgataa aattcagcac ctttgtcacc gtcagcggtt tgctggtatc
    54601 gctgttcttt gcggcgctgg tggagtacat cgtgcgcggc agccgtttct atcaaacctt
    54661 gatgctgctg ccgtatgccg tggctcccgc cgttgccgcc gtattgtgga tcttcctgtt
    54721 taaccccggt cgcgggctga tcacccattt tctcgctgag ttcggctacg actggaacca
    54781 cgcgcaaaac agcggtcagg caatgtttct ggtggtgttt gcctcggtgt ggaagcaaat
    54841 cagctacaac tttctgttct tctatgccgc gctgcactcc attccccgtt cgttgattga
    54901 agccgcagct attgacggcg cagggccgat tcgccgcttc tttaagattg ctctgccgct
    54961 tatcgctccg gtgagtttct tcctgctggt ggtgaacctg gtgtacgcct tcttcgacac
    55021 cttcccggtg atcgacgccg ccacgtccgg cgggccggtt caggccacca ctacgctgat
    55081 ttataagatc taccgcgaag gctttaccgg actggatctg gcttcgtctg ccgcgcagtc
    55141 ggtggtgttg atgttcctcg tcatcgtgct gacggtggtg cagttccgct atgttgaaag
    55201 caaggtgcgt taccaatgat tgagaaccgt ccgtggctga cgatattcag ccataccatg
    55261 ctgatcctcg ggatcgcggt gatcctcttc ccgctgtacg tggcgtttgt cgcggcgacg
    55321 ctggataaac aggccgtcta tgccgcgccg atgacgctca tccccggcac acatctgctg
    55381 gaaaatatcc acaacatctg ggttaacggg gtaggcacga atagcgcgcc gttctggcgg
    55441 atgttgctta acagctttgt gatggcgttc agcattacgc tcggcaaaat taccgtctcg
    55501 atgctctcgg catttgccat tgtctggttt cgttttccgc tacgtaacct cttcttctgg
    55561 atgattttta tcaccctgat gctgccggtt gaagtacgta tcttcccgac ggtggaagtc
    55621 atcgccaacc tgaaaatgct cgacagctac gccggtttaa cgctgccgct gatggcctcg
    55681 gcgaccgcca cgtttctgtt ccgccagttc tttatgaccc taccggatga attggtggaa
    55741 gccgcgcgga tcgacggcgc gtcaccaatg cgtttctttt gcgacatcgt ttttccgttg
    55801 tccaaaacta atctggcggc gctgtttgtg atcaccttta tctacggctg gaatcagtat
    55861 ttgtggccgt tgttgattat taccgacgtt gatctcggca ccaccgtggc agggatcaaa
    55921 gggatgatcg ctacaggcga aggcaccacg gaatggaact cggtaatggc ggcgatgttg
    55981 ttaacgctta tccctccggt ggtgattgtt ttagtgatgc agcgtgcctt cgtgcgcggc
    56041 ctggtcgata gtgagaaata agatggcagg actgaaatta caggcagtaa ccaaaagctg
    56101 ggatggcaaa acccaggtca ttaaaccgct gacccttgat gtggcggatg gcgaatttat
    56161 cgtgatggtc gggccgtctg gctgcgggaa atcgacgctg ctgcgcatgg ttgccgggct
    56221 ggagcgggtg acagaaggcg atatctggat caacgaccag cgcgtgaccg aaatggagcc
    56281 gaaagatcgc gggattgcga tggtgttcca gaattacgcg ctttatccgc atatgagcgt
    56341 agaggagaac atggcgtggg ggctgaaaat tcgcggcatg ggcaagcagc aaattgccga
    56401 gcgcgttaaa gaagcggcgc gcattctgga actggacggt ctgcttaagc gtcgcccgcg
    56461 tgagctatcc ggcggtcagc gccagcgtgt ggcgatgggc cgcgcgattg tgcgcgatcc
    56521 ggcggtgttc ttgtttgatg agccgctctc taacctcgat gccaagctgc gcgtgcagat
    56581 gcgtcttgaa ctgcaacagt tgcaccgtcg cctgaaaacg acttcactct acgttactca
    56641 cgatcaggtt gaagcgatga cgctcgccca gcgagtaatg gtgatgaacg gtggcgttgc
    56701 cgaacagatt ggcacaccag ttgaagtcta cgaaaagccc gccagcctgt ttgtggcgag
    56761 ttttatcggc agcccggcga tgaacctgct gacaggccgc gtgaataatg aaggcacgca
    56821 tttcgaactg gacggcggta tcgtgctacc gctaaacggt ggctaccgtc agtatgccgg
    56881 gcgtaaaatg actctcggca ttcgcccgga acatatagcg ctaagctcgc aggcagaagg
    56941 tggtgtgccg ctggtgatgg acacgctgga gatcctcggc gcagataacc tggcgcacgg
    57001 acgctggggc gagcagaagc tggtggtgcg actggcgcat caggagcgcc cgacggcagg
    57061 cagcacgctg tggctgcatt tggcggaaaa tcagctgcat ctttttgatg gtgaaacagg
    57121 acaacgagta tgagtaactg gccttatccc cgcatcgtcg ctcatcgtgg cggcggtaag
    57181 ctggccccgg aaaacaccct ggcggcaatc gacgtcgggg caaaatacgg tcataagatg
    57241 atcgaatttg acgcgaagtt atcgaaagat ggcgagatct tcctgctcca tgacgacaat
    57301 ctcgaacgca ccagcaacgg ctggggcgtc gcgggtgaac tgaactggca ggatttactg
    57361 cgcgtggatg cgggcagttg gtacagcaaa gcgtttaaag gtgagccgct gccgttgctt
    57421 tcgcaggtgg cggaacgctg tcgcgaacac gggatgatgg cgaatatcga aatcaaaccc
    57481 accactggca ctggaccatt aacgggcaaa atggtggcgc tggcggcacg ccaactgtgg
    57541 gccggtatga cgccgccgct gctgtcatcg tttgagattg atgctttaga agctgcacaa
    57601 caggcggcac cggaactgcc gcgtggttta ttgcttgatg agtggcgcga cgactggcgc
    57661 gaactgaccg cgcggctggg ctgcgtctct attcatctca atcataagtt actcgataaa
    57721 gcgcgagtga tgcagttgaa agacgccgga ttacggattc tggtttatac cgtcaataaa
    57781 ccccagcgcg cggcagagtt gctgcgctgg ggcgtggatt gcatctgtac cgatgcgatt
    57841 gacgtgattg gtccgaactt tacggcccaa tagttttcaa cggaatgtca ggctgcggcg
    57901 tgctgggctg attcagcatg tcgccgtttc tcctttccgg cagcatatgc tgctgattca
    57961 acgaactatc cggattacgg ttgctgttta acatcccgcc gttggtgttg ggcagcattt
    58021 gctgccgggc gggatttcgc tcccccggct gtgactgcaa cacccgctga gaattattgt
    58081 ttatctggtt ttctaaatgc tgctgttgca actgagtttg cgttttcagt tgctgattca
    58141 gcatcccttt ttgctggatt tgctgagtct gcatctgggt ttgcatccgc tgctggctgg
    58201 gtatctgata ccccggctgg ttagggttgt tcagagtatt aatgggctgt gcaaagccga
    58261 caaacggcag gagtgccgta agaatcagaa gtcgtttcat cgcgtatcct cctctgaaga
    58321 tatcctttaa gtttactcgc ttcccgacaa aacgatgatt aattcagagt tatataccag
    58381 gcttagctgg ggttgcccct taatctctgg agaataacga tgataaaacc gacgttttta
    58441 cgccgggtgg ccattgctgc tctgctctca ggaagttgtt ttagcgccgc cgccgcgcct
    58501 cctgcgccgc ccgtctcgta tggtgtggag gaagatgtct tccacccggt acgcgcgaaa
    58561 cagggaatgg tagcgtctgt ggacgccact gccactcagg tgggggtgga tatcctcaag
    58621 gagggcggga atgccgttga tgccgccgtg gcggtgggct acgcgctggc ggtaacgcat
    58681 ccgcaggcag ggaatctggg cggtggtggt tttatgttaa tccgctcgaa aaatggcaat
    58741 accacggcta tcgatttccg cgaaatggca cccgccaaag cgacccgcga tatgttcctc
    58801 gatgatcagg gcaacccgga cagcaaaaaa tcactcactt cgcatctggc ttccggcaca
    58861 ccgggtacgg tagcaggttt ctcgctggcg ctggataaat acggcaccat gccgctgaac
    58921 aaagtcgtgc agcccgcgtt taaactggca cgcgatggtt ttatcgttaa cgacgcgctg
    58981 gctgacgatc tcaaaaccta cggtagcgaa gtgttgccga atcacgaaaa cagtaaagct
    59041 atcttctgga aagagggcga gccgctgaaa aagggcgaca cgctggtgca ggcgaacctg
    59101 gcaaagagcc tggagatgat tgctgaaaac ggcccggacg aattctataa aggcacgatt
    59161 gcggaacaga tcgcccagga gatgcagaaa aacggtggct tgatcactaa agaagattta
    59221 gcggcctata aagcggtcga acgcactccg ataagcggcg attatcgcgg gtatcaggtt
    59281 tactccatgc caccgccatc ctccggcggg atccatatcg tacaaatcct caatattctg
    59341 gaaaacttcg atatgaagaa atacggcttt ggcagcgccg atgcgatgca aatcatggca
    59401 gaagcggaga aatacgccta cgccgaccgc tcggaatatc ttggcgaccc ggattttgtc
    59461 aaagtcccgt ggcaggcact gaccaataaa gcctatgcca aatccattgc cgatcaaatt
    59521 gatatcaaca aagcgaagcc gtccagtgag attcgccctg gcaagcttgc gccttatgag
    59581 agtaatcaaa ctacccatta ctctgtagtg gataaagacg gtaacgcggt ggcggtgacc
    59641 tatacgctga acaccacctt cggtacgggc attgtcgcgg gcgagagcgg tattctgctt
    59701 aataatcaga tggatgattt ctccgccaaa ccgggcgtac cgaacattta cgggctggtg
    59761 ggcggtgatg ccaacgccgt cgggccgaat aaacgtccgc tgtcatcgat gtcaccaacc
    59821 attgtggtga aagacggtaa aacttggctg gttaccggta gtcctggcgg gagtcggatt
    59881 attaccaccg t
//
LOCUS       DAKISA010000012        57474 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.012, whole genome shotgun sequence.
ACCESSION   DAKISA010000012 DAKISA010000000
VERSION     DAKISA010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57474
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.012"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            146..1489
                     /gene="argG"
                     /locus_tag="OVW45_000790"
     CDS             146..1489
                     /gene="argG"
                     /locus_tag="OVW45_000790"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCV5525309.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(1497..3122)
                     /locus_tag="OVW45_000791"
     CDS             complement(1497..3122)
                     /locus_tag="OVW45_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCV5525310.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQSISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPKTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(3581..3667)
                     /locus_tag="OVW45_000792"
     tRNA            complement(3581..3667)
                     /locus_tag="OVW45_000792"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3631..3633),aa:Leu,seq:gag)
     gene            complement(3682..4014)
                     /gene="secG"
                     /locus_tag="OVW45_000793"
     CDS             complement(3682..4014)
                     /gene="secG"
                     /locus_tag="OVW45_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCV5525311.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(4242..5579)
                     /gene="glmM"
                     /locus_tag="OVW45_000794"
     CDS             complement(4242..5579)
                     /gene="glmM"
                     /locus_tag="OVW45_000794"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCV5525312.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(5572..6420)
                     /gene="folP"
                     /locus_tag="OVW45_000795"
     CDS             complement(5572..6420)
                     /gene="folP"
                     /locus_tag="OVW45_000795"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCV5525313.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(6510..8444)
                     /gene="ftsH"
                     /locus_tag="OVW45_000796"
     CDS             complement(6510..8444)
                     /gene="ftsH"
                     /locus_tag="OVW45_000796"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCV5525314.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(8544..9173)
                     /gene="rlmE"
                     /locus_tag="OVW45_000797"
     CDS             complement(8544..9173)
                     /gene="rlmE"
                     /locus_tag="OVW45_000797"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCV5525315.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            9299..9592
                     /gene="yhbY"
                     /locus_tag="OVW45_000798"
     CDS             9299..9592
                     /gene="yhbY"
                     /locus_tag="OVW45_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCV5525316.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(9748..10224)
                     /gene="greA"
                     /locus_tag="OVW45_000799"
     CDS             complement(9748..10224)
                     /gene="greA"
                     /locus_tag="OVW45_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCV5525317.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            10472..11905
                     /gene="dacB"
                     /locus_tag="OVW45_000800"
     CDS             10472..11905
                     /gene="dacB"
                     /locus_tag="OVW45_000800"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCV5525318.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAQKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(12091..13263)
                     /gene="cgtA"
                     /locus_tag="OVW45_000801"
     CDS             complement(12091..13263)
                     /gene="cgtA"
                     /locus_tag="OVW45_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCV5525319.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFILE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(13279..14244)
                     /locus_tag="OVW45_000802"
     CDS             complement(13279..14244)
                     /locus_tag="OVW45_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCV5525320.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(14371..14628)
                     /gene="rpmA"
                     /locus_tag="OVW45_000803"
     CDS             complement(14371..14628)
                     /gene="rpmA"
                     /locus_tag="OVW45_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCV5525321.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(14649..14960)
                     /gene="rplU"
                     /locus_tag="OVW45_000804"
     CDS             complement(14649..14960)
                     /gene="rplU"
                     /locus_tag="OVW45_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCV5525322.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            15219..16190
                     /gene="ispB"
                     /locus_tag="OVW45_000805"
     CDS             15219..16190
                     /gene="ispB"
                     /locus_tag="OVW45_000805"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCV5525323.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            16418..16696
                     /gene="sfsB"
                     /locus_tag="OVW45_000806"
     CDS             16418..16696
                     /gene="sfsB"
                     /locus_tag="OVW45_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCV5525324.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(16744..18003)
                     /gene="murA"
                     /locus_tag="OVW45_000807"
     CDS             complement(16744..18003)
                     /gene="murA"
                     /locus_tag="OVW45_000807"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCV5525325.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGVHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(18058..18312)
                     /gene="ibaG"
                     /locus_tag="OVW45_000808"
     CDS             complement(18058..18312)
                     /gene="ibaG"
                     /locus_tag="OVW45_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCV5525326.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(18472..18765)
                     /gene="mlaB"
                     /locus_tag="OVW45_000809"
     CDS             complement(18472..18765)
                     /gene="mlaB"
                     /locus_tag="OVW45_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCV5525327.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(18765..19400)
                     /gene="mlaC"
                     /locus_tag="OVW45_000810"
     CDS             complement(18765..19400)
                     /gene="mlaC"
                     /locus_tag="OVW45_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCV5525328.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(19419..19970)
                     /gene="mlaD"
                     /locus_tag="OVW45_000811"
     CDS             complement(19419..19970)
                     /gene="mlaD"
                     /locus_tag="OVW45_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCV5525329.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(19975..20757)
                     /gene="mlaE"
                     /locus_tag="OVW45_000812"
     CDS             complement(19975..20757)
                     /gene="mlaE"
                     /locus_tag="OVW45_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCV5525330.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(20765..21574)
                     /gene="mlaF"
                     /locus_tag="OVW45_000813"
     CDS             complement(20765..21574)
                     /gene="mlaF"
                     /locus_tag="OVW45_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCV5525331.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            21784..22761
                     /locus_tag="OVW45_000814"
     CDS             21784..22761
                     /locus_tag="OVW45_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCV5525332.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIEPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            22775..23761
                     /gene="kdsD"
                     /locus_tag="OVW45_000815"
     CDS             22775..23761
                     /gene="kdsD"
                     /locus_tag="OVW45_000815"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCV5525333.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            23782..24348
                     /gene="kdsC"
                     /locus_tag="OVW45_000816"
     CDS             23782..24348
                     /gene="kdsC"
                     /locus_tag="OVW45_000816"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCV5525334.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRANYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            24345..24920
                     /gene="lptC"
                     /locus_tag="OVW45_000817"
     CDS             24345..24920
                     /gene="lptC"
                     /locus_tag="OVW45_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCV5525335.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGIDMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            24889..25446
                     /gene="lptA"
                     /locus_tag="OVW45_000818"
     CDS             24889..25446
                     /gene="lptA"
                     /locus_tag="OVW45_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCV5525336.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            25453..26178
                     /gene="lptB"
                     /locus_tag="OVW45_000819"
     CDS             25453..26178
                     /gene="lptB"
                     /locus_tag="OVW45_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCV5525337.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            26226..27659
                     /gene="rpoN"
                     /locus_tag="OVW45_000820"
     CDS             26226..27659
                     /gene="rpoN"
                     /locus_tag="OVW45_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCV5525338.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDIGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHINTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            27682..27969
                     /gene="hpf"
                     /locus_tag="OVW45_000821"
     CDS             27682..27969
                     /gene="hpf"
                     /locus_tag="OVW45_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCV5525339.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            28087..28578
                     /gene="ptsN"
                     /locus_tag="OVW45_000822"
     CDS             28087..28578
                     /gene="ptsN"
                     /locus_tag="OVW45_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCV5525340.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVSADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            28624..29478
                     /gene="rapZ"
                     /locus_tag="OVW45_000823"
     CDS             28624..29478
                     /gene="rapZ"
                     /locus_tag="OVW45_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCV5525341.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            29475..29747
                     /gene="npr"
                     /locus_tag="OVW45_000824"
     CDS             29475..29747
                     /gene="npr"
                     /locus_tag="OVW45_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCV5525342.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            29961..30593
                     /gene="yrbL"
                     /locus_tag="OVW45_000825"
     CDS             29961..30593
                     /gene="yrbL"
                     /locus_tag="OVW45_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCV5525343.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKLSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(30590..31318)
                     /gene="mtgA"
                     /locus_tag="OVW45_000826"
     CDS             complement(30590..31318)
                     /gene="mtgA"
                     /locus_tag="OVW45_000826"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HCV5525344.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(31315..31968)
                     /gene="elbB"
                     /locus_tag="OVW45_000827"
     CDS             complement(31315..31968)
                     /gene="elbB"
                     /locus_tag="OVW45_000827"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCV5525345.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(32198..34534)
                     /gene="arcB"
                     /locus_tag="OVW45_000828"
     CDS             complement(32198..34534)
                     /gene="arcB"
                     /locus_tag="OVW45_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCV5525346.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(34630..35559)
                     /locus_tag="OVW45_000829"
     CDS             complement(34630..35559)
                     /locus_tag="OVW45_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCV5525347.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLINRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTVHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGLAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            36234..40694
                     /gene="gltB"
                     /locus_tag="OVW45_000830"
     CDS             36234..40694
                     /gene="gltB"
                     /locus_tag="OVW45_000830"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCV5525348.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGHILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENEVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIACE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            40707..42125
                     /gene="gltD"
                     /locus_tag="OVW45_000831"
     CDS             40707..42125
                     /gene="gltD"
                     /locus_tag="OVW45_000831"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCV5525349.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            42309..42788
                     /locus_tag="OVW45_000832"
     CDS             42309..42788
                     /locus_tag="OVW45_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HCV5525350.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
     gene            42805..43110
                     /locus_tag="OVW45_000833"
     CDS             42805..43110
                     /locus_tag="OVW45_000833"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCV5525351.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(43186..43650)
                     /locus_tag="OVW45_000834"
     CDS             complement(43186..43650)
                     /locus_tag="OVW45_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCV5525352.1"
                     /translation="MMMGEVRSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIENEQAIILKPGMFAVFMLGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(43647..44522)
                     /gene="nanK"
                     /locus_tag="OVW45_000835"
     CDS             complement(43647..44522)
                     /gene="nanK"
                     /locus_tag="OVW45_000835"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCV5525353.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAVFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(44519..45208)
                     /locus_tag="OVW45_000836"
     CDS             complement(44519..45208)
                     /locus_tag="OVW45_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCV5525354.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPIPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(45256..46746)
                     /gene="nanT"
                     /locus_tag="OVW45_000837"
     CDS             complement(45256..46746)
                     /gene="nanT"
                     /locus_tag="OVW45_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCV5525355.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(46856..47749)
                     /gene="nanA"
                     /locus_tag="OVW45_000838"
     CDS             complement(46856..47749)
                     /gene="nanA"
                     /locus_tag="OVW45_000838"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCV5525356.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(47871..48662)
                     /gene="nanR"
                     /locus_tag="OVW45_000839"
     CDS             complement(47871..48662)
                     /gene="nanR"
                     /locus_tag="OVW45_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCV5525357.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGEFSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            49042..50409
                     /gene="dcuC"
                     /locus_tag="OVW45_000840"
     CDS             49042..50409
                     /gene="dcuC"
                     /locus_tag="OVW45_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCV5525358.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(50452..50949)
                     /gene="sspB"
                     /locus_tag="OVW45_000841"
     CDS             complement(50452..50949)
                     /gene="sspB"
                     /locus_tag="OVW45_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCV5525359.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(50955..51593)
                     /gene="sspA"
                     /locus_tag="OVW45_000842"
     CDS             complement(50955..51593)
                     /gene="sspA"
                     /locus_tag="OVW45_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCV5525360.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIELSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(51988..52380)
                     /gene="rpsI"
                     /locus_tag="OVW45_000843"
     CDS             complement(51988..52380)
                     /gene="rpsI"
                     /locus_tag="OVW45_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCV5525361.1"
                     /translation="MAENQYYGTGRRKNSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(52396..52824)
                     /gene="rplM"
                     /locus_tag="OVW45_000844"
     CDS             complement(52396..52824)
                     /gene="rplM"
                     /locus_tag="OVW45_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCV5525362.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQVSFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(53043..54170)
                     /gene="zapE"
                     /locus_tag="OVW45_000845"
     CDS             complement(53043..54170)
                     /gene="zapE"
                     /locus_tag="OVW45_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCV5525363.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            54364..54762
                     /locus_tag="OVW45_000846"
     CDS             54364..54762
                     /locus_tag="OVW45_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCV5525364.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            54916..56283
                     /gene="degQ"
                     /locus_tag="OVW45_000847"
     CDS             54916..56283
                     /gene="degQ"
                     /locus_tag="OVW45_000847"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HCV5525365.1"
                     /translation="MKKQPQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPEQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMIAPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKSAIIALQIVRGNESI
                     YLLMR"
     gene            56373..57440
                     /gene="degS"
                     /locus_tag="OVW45_000848"
     CDS             56373..57440
                     /gene="degS"
                     /locus_tag="OVW45_000848"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCV5525366.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
BASE COUNT        14267 a        15313 c        14273 g        13621 t
ORIGIN      
        1 agcgcattat tttacaaaaa acacactaaa ctcttcctgt ctccgataaa agatgattaa
       61 atgaaaactc atttattttg cataaaaatt cagtgagagc ggaaatccag gctcatcatc
      121 agttaattaa gcagggtgtt attttatgac gacgattctc aagcatctcc cggtaggtca
      181 acgtattggt atcgcttttt ccggcggtct ggacaccagt gccgcactgc tgtggatgcg
      241 acaaaaggga gcggttcctt atgcatatac tgcaaacctg ggccagccag acgaagagga
      301 ttatgatgcg atccctcgtc gtgccatgga atacggcgcg gagaacgcac gtctgatcga
      361 ctgccgcaaa caactggtgg ccgaaggtat tgccgctatt cagtgtggcg catttcataa
      421 caccaccggc ggcctgacct atttcaacac gacgccgctg ggccgcgccg tgactggtac
      481 catgctggtt gctgcgatga aagaagatgg cgtgaatatc tggggtgacg gtagcaccta
      541 taaaggaaac gatatcgaac gtttctatcg ttatggtctg ctgaccaatg ctgaactgca
      601 gatttacaaa ccgtggcttg atactgactt tattgatgaa ctgggcggcc gtcatgagat
      661 gtctgaattt atgattgcct gcggtttcga ctacaaaatg tctgtcgaaa aagcctactc
      721 cacagactcc aacatgcttg gtgcaacgca cgaagcgaag gatctggaat acctcaactc
      781 cagcgtcaaa atcgtcaacc caattatggg cgtgaaattc tgggatgaga gcgtgaagat
      841 cccggcagaa gaagtcacag tacgctttga gcaaggtcat ccggtagcgc tgaacggtaa
      901 aacctttagc gacgacgtag aaatgatgct ggaagctaac cgcatcggcg gtcgtcacgg
      961 cctgggcatg agcgaccaga ttgaaaaccg tatcatcgaa gcgaaaagcc gtggtattta
     1021 cgaagctccg gggatggcac tgctacatat cgcttatgaa cgtctgttga ccggtattca
     1081 caacgaagac accattgagc agtatcatgc acatggtcgt cagttgggcc gtctgctgta
     1141 ccaggggcgt tggtttgatt cccaggcgct gatgctgcgt gactctctgc aacgctgggt
     1201 tgccagccag atcactggtg aagttaccct ggagctgcgc cgtgggaacg attattcaat
     1261 cctgaatacc gtctcagaga acctgaccta caagccagag cgtctgacga tggaaaaagg
     1321 cgactcggtg ttctcgccag atgatcgtat tggtcaattg accatgcgta acctggatat
     1381 cactgatacc cgagagaaac tttttggtta tgccaaaact ggcctgctct cctcctctgc
     1441 cacttcaggc gtgccgcagg tggagaatct ggaaaacaaa ggccagtaat tcgacatcaa
     1501 ccctgccctt caggggtggg gttaccttgc ttatcctctg taaactcctt tctgagcatc
     1561 acataatcga aaacatcatg gtttgcatcc acaacccgtg agtctccttt atagtgtgca
     1621 atcacttcct ccagcgtttt cggttgttcc ggctttgtta cccccatcca cgcattaata
     1681 aggtaattat tgtaagctgt cgaaaagatg ttgttttccg ttgtacgatc cacgccatcc
     1741 ccaagaacgg ggctatacca gataaacatc gggacatgat atgcctgctg gctagcctcc
     1801 ctgcctccat gaaaatagac gttcttcttc gtagggtcac gttccaggcc gtggtcggca
     1861 aaatacatga ccgaggcgcg gcgatctttt aataattcaa aaacctgacc tagcaaacta
     1921 tctgtgtaat gaatggagtt gtcatagcag gcatcctgat cgtcctgcgg ttgaaacacg
     1981 gcgctggatt gcggataggc gctacaagcc ggttcatggc ttccatttaa atgaagaaca
     2041 atcagtttct tttgctgcgt attttgctgt aatgcttgcg ataaatgcgg caacaacaat
     2101 tcatcaaatc ctctgacata aactgtttcc atggcgcgca tggcgatact ggtaactgct
     2161 gtaccattct gccgaaaagc ggattgcgag cttagccaga aagtctgaaa tcctgcttga
     2221 ttagccatat taataatgtt gtcggggtaa ttatgaatgt catgactcaa aacagaatca
     2281 gcagttaaag aaaggggaac cgacagcgca gtgtaaggtg cgccgcttat tgattgatta
     2341 aacagtttga tctgttttct ttgtgcttca acttgcggtg tcgtagagcg tgtatatcca
     2401 tacaaagaca tattgtcgac acgtacagac tcccccacaa tcaacacgta ggtatcaata
     2461 cctgtatccc tgactgataa ttgaaaatac ggcaccgtgt ttgcaattga gagtaatctt
     2521 tgatgctctt tcgctgctaa agcaaaataa ttgagattga aaaacggcgt ataggtagca
     2581 aatcgcgacg ctagtatata tggactgaat gcttttttat tttttgcatc tttataagca
     2641 aattgacaag cagaaaataa actgcccgaa atgacaatca gcaataatat tcctgtcact
     2701 ttttttgtcg gcaaggaaac atcatatttt attattactg ccaaaaaaag aagggataaa
     2761 aaagcaaaag cacatagata agggatatac atccccagca ttttgacaac ttcatccgga
     2821 tcactctgca gcacactaat cgcaaatcca tcattgaaag ttgtaccaaa ggtacaccat
     2881 gaatagaggc taatactcat gtctgccgca gtcagtaaca caaatggtgc ggcaacaagc
     2941 aggcgaagcg gaagggatga tatacgtttt acaaccagga tggtcaggac gaagaacagc
     3001 aatcgctgaa ccatatgcgc gccggaagag gcgactaagt tcgtaatacc aaaaacaata
     3061 acacaaagat acaacaacca atgagattta aaacttctag caaatttgtt gaatactgtc
     3121 atccagaaat ccaagttcac aaccaatata aacaattatc aacaatgttc aattttatat
     3181 cagacatgtt tttcaactct gatccatatc agaccgaaac actgttagtt ttttcttttt
     3241 aaatgatgcg gccattgcgt tctcagtaat taaataacat tatcaatgcg tctctaaaca
     3301 ttacgcagaa cgtagccatc agtagttata ctgacaggag caggatggtt tatcgagaag
     3361 taaacatatc cgtattcgta ccggaactgg agttgacaca ataaagtgcc aattatgtca
     3421 gcagaaggga aaagtttata actaaggcgt accggcacca tcgtttcaag gtaccagcta
     3481 cgagtaaagc aactggacga gatacagata cctgacaacc attcctcaga ccaggaccaa
     3541 aacgaaaaaa gacgcttttc agcgtctctt ttctggaatt tggtaccgag gacgggactt
     3601 gaacccgtaa gccctattgg gcactaccac ctcaaggtag cgtgtctacc aattccacca
     3661 cctcggcacg gatactactt tttagttcgg gatatcgctg gtcggcttag ccggagcagc
     3721 tggctgagtt tgttcggttt tcgccggtgc actcagattt tcccattcgc tacctttatt
     3781 ggttttgttg ctattgatgt tacccagcac cagactgatg atgaagaata acgttgccag
     3841 cagcgccgtc atgcgggtca tgaagttacc agaaccactt gaaccaaaca gcgtagcgga
     3901 agcgcctgct ccgaaggagg ctcccatatc agcgccttta ccttgctgca gcatgatcag
     3961 accaacaagg ccaattgcca caataaggaa aactaccaaa agagcttcgt acataatcaa
     4021 cctgttcctt gcggagttgc cgcgtaccaa tgcttcaacc aataaagcgg gagtttttaa
     4081 tctttcccac tgaagtgggt gtgaatacta accaaagcga atgaccttcg caagggcaat
     4141 tttatcgcat tgtatcaact gcggaaaaaa acagcaaaac cgatgtgttc gctgagaaaa
     4201 aaggcgacaa tcgccgcctt tttagccagt tatttaccgt tttaaacggc ttttactgca
     4261 tcggcgatac ggtgtgcaaa ttcagttacc tgcgcttcgt cttcaccttc taccatcacg
     4321 cgaattaacg gttcggtgcc ggatttacgc aacagcacac gtccacggct acccagcgcc
     4381 gcttcaactt cggcggtcac agctttaact gactcatgct caagcggatc gccgctacct
     4441 gcggtgtaac gcacgttaac cagaatctgc gggaacattt tcatgccgct gcaaaggtcg
     4501 tgcaggctca tatggttacg tgccatcgcc gccagcacct gcaagccagc aacgatgccg
     4561 tcaccggtag tggttttatc cagcaggatc acatgaccgg aattctctgc accgatacgc
     4621 cagcctttct cctgcatttt ttccagtacg tagcggtcac ccactttcgc gcgcgcaaat
     4681 ggaattccta actgtttcaa cgccagctca agccccatgt tactcatcaa tgtacccaca
     4741 gcaccaccac gcagctggcc ctgacgaaga ccttcacgcg cgatgatata catgatctga
     4801 tcaccatcga ctttattgcc ttcatggtca accataatca cgcgatcgcc gtcgccgtcg
     4861 aaggcaatac cgagatccgc tttttcagcc agcacacgag cctggagcgc gcgaacgtcg
     4921 gtagccccca cttcggcatt gatgtttaca ccgttcggct cacaaccgat agcgataacg
     4981 ttcgccccca gttcacgcag cacgttcggc gcgatgtgat aagtcgcacc gtttgcgcaa
     5041 tccaccacaa tcttcagttc gctgaggcta agttcgttcg ggaacgtggc tttgcaaaac
     5101 tcgatatagc gacccgcagc atcaacgata cggctggctt tacccagttc tgccgaatca
     5161 acacagctga tctctttttc catttccgct tcgatggcct cttctaccgc atccggtagt
     5221 ttggtgccgt cgatagagaa aaatttaatg ccattatcgt agaacgggtt atgcgatgca
     5281 gatatcacaa ttccggcctc tgcgcggaag gtacgcgtca gataagccac ggccggtgtt
     5341 ggcatcgggc cagtgaagag tgcggaaagg cccgctgccg ccagacccgc ttccagtgct
     5401 gactccagca tatagccaga aatacgcgtg tctttaccaa taatgatctt acgggagccg
     5461 tggcgcgcca gcactttacc cgcggcccaa cccagcttaa gcacaaaatc aggtgtgatc
     5521 ggcgcatccc ctacacgacc acgaatccca tcggtaccga aatatttacg attactcata
     5581 gcgtttgttt tcctttgcag acagagtggc ttccaccacc cgcatcgctt ctacggtttc
     5641 tttgacgtca tgaacacgaa taatgtgcgc gccttgcatt gcggcaatga ccgcacaggc
     5701 cagactaccg ctcaggcgct cggacggccc cacgttcagc agctgcccaa tcatcgattt
     5761 tcgtgacata cctaccaaca gcggcaggtt gaaatggtga aattcagcca ggcgcgccag
     5821 taatgaatag ttatgggaga gatttttacc gaaaccgaat ccggggtcga gcaacaattt
     5881 ctcttttgcg atacccgcct gctcgcaacg tgctatttgc tcaataaagt agcgattcac
     5941 ttctgcaaag acatcgtcat acttcggagc ttcctgcatg gtttttggat ttccctgcat
     6001 atgcatcaga caaaccggta aaccggtttc tgcagccgcc tccagagcgc caggttcgga
     6061 aagggagcgg atatcgttaa taatgtgagc gccaactttc gctgactcac ggatgacttc
     6121 tggtttggat gtatctaccg agatccagac ttcgaagcgt tgagcaattg cctcaaccac
     6181 aggaataaca cgttgcaact cttcttcaac gctaacttcc gccgcccctg ggcgcgtgga
     6241 ctcgccacca acgtcaatga tcgtcgcgcc agcgttgatc atcagattcg catgtttcac
     6301 cgcatctatc agcgagttat gcgtgccacc atccgaaaag gaatcaggcg tgacgttgag
     6361 gatccccatt acgtgaggat ggctaaggtc cagtgaagtg ccctgggcaa agagtttcat
     6421 ggtgttatcc ctggtatgaa ttgataagaa aaaaccccgg agcacgcccc ggggttttcg
     6481 gtacaaatac agtcatctga tgcgggaact tacttgtcgc ctaactgctc tgacatggtg
     6541 ttacccgggt tcggcgtacg cggttcatca accggacgag gagcctttgg actaccattg
     6601 tcgccagaat tgttagaagc gcctggttct tcccagcccg ctggcggacg tacatcgcga
     6661 cgtgccatca ggtcatcaat ctgcggtgcg tcgatagtct catatttcat gagagcatct
     6721 ttcatcgcat gcagaatatc catattgtcg gtcagaagct gacgcgcacg attatagtta
     6781 cgctcaatca gtgctttcac ttcctggtcg atgatacgtg cagtttcatc ggacatatgt
     6841 ttcgctttcg ctacgctacg gccgaggaac acttcacctt cttcttccgc gtacagcagt
     6901 ggacccaatt tctcagagaa gccccactga gtcaccatgt tacgcgccag gttggtcgca
     6961 actttaatat cgttggacgc accggtagaa acatgttccg gcccgtagat gatctcttct
     7021 gccagacgac caccgtacag cgtagaaatc tggctttcca gtttctgacg gctggcgctg
     7081 attgcgtcgc cctcaggcaa gaagaaagtc acacccagcg cacgaccgcg tgggataatc
     7141 gtcactttgt gcaccggatc gtgttccggc accaggcgac cgataatcgc atgacccgct
     7201 tcgtggtaag ccgtcgattc tttctgcgct tccgtcatca ccatggagcg acgttccgca
     7261 cccatcatga ttttatcttt cgctttctcg aactcaacca tcgacacaac gcgtttgttg
     7321 ccacgagcag cgaacagtgc cgcttcgttt accaggttcg ccaagtcagc accggagaaa
     7381 ccaggagtac cacgggcaat gattgccgcg tcgatatcgg gtgccaatgg tacgcgacgc
     7441 atgtgaactt tcaggatctg ctcacgaccg cgaacatctg gcaagccaac cacaacctga
     7501 cggtcgaaac ggccaggacg cagcagggcc gggtcaagaa cgtccggacg gttagtcgcg
     7561 gcgataacga tgataccttc gttaccttcg aagccatcca tctcaaccag catctggttc
     7621 agagtctgtt cacgttcatc gtgaccaccg cccagaccag cgccacgctg gcggcctacg
     7681 gcgtcgattt catcgataaa gatgatgcac ggtgccgctt tcttcgcctg ttcgaacatg
     7741 tcacgaacac gggatgcacc cacaccgacg aacatttcta cgaagtcaga accggagata
     7801 gtaaagaacg gaactttcgc ttcgcctgca atcgctttcg ccagcagcgt tttaccggta
     7861 cccggaggac cgaccatcag gacgcctttc gggatcttac cgccgagttt ctggaagcgg
     7921 ctcggctcgc gcagatattc aaccagttca gcaacttctt cttttgcttc gtcgcagccc
     7981 gcaacgtcag caaaggtcgt tttgatctga tcttccgtca gcatgcgcgc tttgctctta
     8041 ccaaacgaca tggcaccttt gccaccgccg ccctgcattt gacgcatgaa gaagatccac
     8101 acaccaatca gcaacagcat cgggaaccag gagatgaaga tagaagccag caggcttggt
     8161 tcttcaggcg gttcaccgac aaccttgacg ttcttggtca acaggttatc cagtaatttc
     8221 ggatcctgaa ccggaatgta agtggtataa cggttactat ctttcttggt aacgttgatt
     8281 tcacgtccgt tgatacgcgc ttcacgaacc tggtcgttat tgacctcttg taggaaggta
     8341 gagtaatcca ccttacggcc attagactcg ctgggcccaa agctctggaa tactgacatc
     8401 agcacaacgg caatgaccag ccagagtatt aggtttttcg ccatgtcact caagggatta
     8461 acctcttatt acaactgtgt taaaaacagc gtcaggatac tctatatcca gcatctttca
     8521 aactttcgtc tgaaatctcc cggttagggt ttacgcccgg tcgctacaat atacacttcc
     8581 cgcgaacgtg cacgagaaga gtccggctta cgaactttga ccttcgtaaa cagggagcga
     8641 atttccctta gatactcatc gaaaccttcg ccctggaaca ccttcactac aaaactgcca
     8701 cctggcgcta atacatcacg acacatttct agcgccagtt ccaccagata catggcacgg
     8761 gggatatcca ccgccggtgt tccgctcatg tttggtgcca tatcggacat gacaacctgg
     8821 actttgctgt cgccaacgcg ctccagcagt gctttcatca ccagttcatc acgaaaatcg
     8881 ccctgaagaa agtccacacc aacgatagga tccataggta aaagatcgca agcgatgatg
     8941 cggcctttgc cgccaatttg ggtgaccaca tattgtgacc aaccacccgg agcagcacca
     9001 aggtcgacaa ccgtcattcc cggtttaaag agtttgtcac tttgctgtat ttcatcaagt
     9061 ttaaaccagg cacgggaacg taaccccttt ttctgtgcct gttgaacata tttatcgcta
     9121 aagtgttcct gaagccagcg gctggagctg gcagaacgct tcttacctgt catttaactt
     9181 tcccatgggg ataactcatc gtaaccaatt gcgtaaattt ttacgcgcct atttggtgat
     9241 atatgggaga tggcggtaga atgacccgtt ttcaatccca acgtaagcaa aaaatacgat
     9301 gaatctgagt actaaacaaa aacagcacct gaaaggtctg gcacatccgc tcaagccagt
     9361 tgttctgctt ggcagtaatg gtttgaccga aggggtgctg gccgagattg aacaagcgtt
     9421 agagcaccat gaactcatca aggtgaaaat cgccaccgaa gatcgcgaaa caaaaacctt
     9481 gatcgtggaa gctatcgtgc gcgaaaccgg cgcctgtaat gtacaggtca tcggtaaaac
     9541 gctggtgctt tatcgcccaa ctaaagaacg taaaatctcg ctgccacgct aagattatcc
     9601 taaagttaca cacatttgct gtgtaaaacg aggggttttc cgcaggcagg agagcaaaat
     9661 gccacgctct gttcgttgat aaaaggccgc atagcggcct ttttcctttc tttacaatac
     9721 atcaacatct tgagtattgg gtaattctta caggtattcc accttaatta cttcaaattc
     9781 tacttcgccg cccggcgttt tgatgaccac aacatcatct tcttctttgc cgatcaggcc
     9841 acgagcaata ggcgagttta cagaaatcag gttttgttta aagtcagctt cgtcatcgcc
     9901 aacgatgcga taagtctgtt cttcgtcaga atccagattc agcaccgtta cggtagcacc
     9961 aaaaataacg cgcccattgt taggcatttt ggtgacatca atcacctgcg cgtttgacag
    10021 cttggcttcg atgtctttga tacggccttc gcagaagccc tgctgttcac gagctgcgtg
    10081 gtattcggcg ttttctttca ggtcgccatg ctcacgcgct tccgcgatag cagcaatgat
    10141 ttcaggacgg cgcacagatt tcagaaaatc cagctcttcg cgtaattttt cagcgccgcg
    10201 taaggtcatc ggaatagctt gcatttgtta tacctcttga atattcctga tggggcaagt
    10261 cttcacccac cccggctgtt cggcctgccc ggcataactc ctcaccggga ccagaagcaa
    10321 aaaaataccg acccaggtac aagtcccagg tcagctacaa ttcacatttt gatagtcatt
    10381 ttaccctgaa gttcccgaag ggtcatcgtt tactttatag ggcgttgcgc cgtagtatga
    10441 cggctcgatt ccaggttgtt agcgcgagat tatgcgattt tccagattta tcatcggatt
    10501 gaccagctgt atagcgttca gtgttcaggc cgcaaatgtt gatgagtaca ttactcaact
    10561 ccccgctggt gccaaccttg ccctgatggt gcaaaaagtc ggcgcgtcgg cccccgctat
    10621 tgattaccac agtcagcaga tggcgctgcc tgccagtacc cagaaagtga ttactgcgct
    10681 ggcggcgttg attcaactcg gccccgattt tcgttttacc acgacgcttg aaaccaaagg
    10741 caatgtggaa aacggcgtac ttaagggtga cttagtggcg cgatttggtg ccgatccgac
    10801 gttaaaacgt caggatattc gcaatatggt cgcgactttg aaaaaatctg gcgtcaacca
    10861 aatcgatggc aatgtgttga tagatacctc cattttcgcc agccacgata aagcccccgg
    10921 ctggccatgg aatgacatga cacaatgctt tagcgctccg cctgccgccg ccatagttga
    10981 ccgcaactgt ttctccgtct cgctctacag tgcccaaaaa cccggtgata tggcctttat
    11041 acgcgtggca tcttattacc ccgttacgat gttcagccag gtacgcaccc tcccccgtgg
    11101 ttctgccgaa gcgcaatact gcgaactgga tgtcgtacca ggcgacctga accgctttac
    11161 gctgacggga tgcctgccac aacgttctga gccgctcccg ttggcttttg ccgtgcagga
    11221 tggagccagt tatgccggtg caattctgaa agatgagtta aaacaggcgg gtatcacctg
    11281 gagcggaaca ctgctgcgcc agactcaggt taacgaacct ggaacggtag ttgccagtaa
    11341 acagtcggcc ccgctgcacg atctgcttaa gattatgctg aaaaagtcgg ataacatgat
    11401 cgccgatacg gttttccgca tgataggcca tgcgcgcttc aatgtacctg gtacatggcg
    11461 ggccgggtcg gacgctgtgc gtcagatcct gcgccagcaa gccggtgtcg atattggaaa
    11521 caccattatt gccgatggtt cagggctttc gcggcataac ctgattgccc ccgccaccat
    11581 gatgcaggtg ctgcaataca ttgcccaaca cgacaatgaa cttaacttta tctccatgct
    11641 gccgctggcg ggctatgacg gctctttgca gtaccgtgca ggtctgcatc aggcgggcgt
    11701 ggatggaaaa gtctcagcga aaaccggttc gttgcagggg gtatataacc tggcgggatt
    11761 cattaccaca gcgagcgggc aacgaatggc gtttgtgcaa tatctttctg gctatgcagt
    11821 agaacctgcg gatcagcgta atcgccgtat tccgttagtg cgttttgaaa gccgtttgta
    11881 taaagatatt tatcagaaca attagtcaaa aagaaacccc ggcacatggc cggggcttca
    11941 gattattgac aaagtgcgct ttgtttatgc cggatgcggc gtaaacgcct tatccggcct
    12001 acaaaatcgt gcaaattcaa catattgcaa ttctcttgta ggcctgataa gcgtagcgca
    12061 tcaggctgat ttggcgttta tcatcagtga ttaacgcttg taaatgaact caacgccttc
    12121 ttcgtcgtct tcgtcccagt cgtcatccca gtcttcatca tcctcttcag caatctcttc
    12181 aagctgctgg cgatgataat cgtcccacat gaattcgact ttctctggct gtttcgcttc
    12241 ttcagcctgc acgaccgggt tttcaaggat aaaggtcatc acatcccagc agagatcttt
    12301 cacgcccagt ccgctcgccg cagagatcag ataatattta tcttcccagc ccagcgcttc
    12361 agcaatcgct ttcgctttct cttcagcttc tgccttatcc agcagatcga tcttattgaa
    12421 cactaaccaa cgcggtttcg ccgccagatc ctggctgtat ttttccagct cgctgataat
    12481 aatacgcgcg ttttcaaccg gatcggtgcc gtcaatcgga tcgatatcga tgaggtgcaa
    12541 caggacgcgg caacgttcca ggtgcttcag gaagcggata cccagacctg cgccttccgc
    12601 agcgccttca atcagtcctg gaatatcggc aacaacgaag ctcttttcgt tgtccattcg
    12661 taccacaccc agacttggca ccagagtggt aaacggataa tccgccactt tcggtttagc
    12721 cgccgatacc gcgcgaataa aggtcgattt acccgcgttt ggcatcccca acataccgac
    12781 gtcagccagc agcatcagct ccagcagcag ctcacgctta tcacccggcg taccgttggt
    12841 tttctgccgt ggtgtacggt taacggacga tttgaaacgg gtattgccca gaccgtgcca
    12901 gccgccctta gcaaccagca gacgctgacc gtgtttagtc atatcgccca tggtttcacc
    12961 agtaccctgg tcgattacac gcgtacctac cggcacttta atcgtcacgt ctttaccgcg
    13021 cttaccggta caatcgcggc ttgcgccgtt ctgaccgcgc tctgcacgga aagatttttc
    13081 aaaacgataa tcgataagcg tgttcaggtt ttcgtcggct tccatccata cgtcaccacc
    13141 atcaccaccg tcgccgccat ccgggccgcc tttcggaata tacttttcgc ggcggaagct
    13201 cacgcaacca ttaccgccat cacctgcaac gaccagaatc gatgcttcat caacaaactt
    13261 cattttattc tccataaatc attcgcctgc gcggggttgc gataccaccg ttgtatgctt
    13321 acgtaatccg ccccaaatac gatgaccaat ggcggaatac atcgcgcccg caaccacgac
    13381 aaacgcaccg agataaccta aaaggtttaa catcggtctg gcgaagaaat cgggccaggc
    13441 cagtgataaa agatctgaaa aaaacagcgt aaacagtggg gtgagcgtga tgatcgcgct
    13501 cacctgcgct gcctgccagc gagccatcgc ttccgccagg gcgccatatc ctaccaatgt
    13561 attcagtccg caaaaaatta aacatgcgag ctgccagtgg ctaagctgcg ctatcactcc
    13621 aggctttgcc agagggaaga gcgcaattgt acataaagtg tacagtaaaa acaggatctg
    13681 cggtgaggcc agccgacgca ataaaacctt ttgcgccacg ccataactca cccaaaccgt
    13741 cgccgcaccg accccaaaga taactcccca ggtgtaatcg gtgagctttg taaatatctc
    13801 gaccagactg gtgttaaaaa acatcaccag gccgctcagg agcatcaatg cccctacaac
    13861 ctgagtgctg cgcattttct ctttcaggat aaatacgctg gcaaccatca tgccaactgg
    13921 cgagagttgc ccaatcacct gcgaagcggt cggactcagg tattgcaagg atgagctgaa
    13981 caggatgaag ttcccaaaca gcccggcggt cgccactgcc aacaaaatca accagcgtgg
    14041 cttacgaaac acgcgtaatg gcggcaacct cttcttcacc gcaagaatgg caccaaggcc
    14101 aatactcgcc atcaagaaac ggtaaaacac gattgtcgga ggttccatca cctccagcac
    14161 ctgcttcatt gcgattggca acgcccccca gcaaattgct gtggtgagcg ccaaaagaat
    14221 gccaatgcct gcctgctgct tcataccgta ttccctgcaa agcgcatttg cggattaacg
    14281 tcgcaccatg cgcattttta ccggttatcg aatgtaaaaa gccccgcaac gtgttgcggg
    14341 gctttcatcc gttaccggga cgcgaaaaac ttattcagct tcgatgctga taaatttacg
    14401 gtttttcggg cctttaactt cgaatttcac tttaccgtct gctttagcaa acagagtgtg
    14461 gtcacgaccg caacctacgt tagcgccagc gtggaatttg gtaccacgtt gacgaacgat
    14521 gatgctaccc gccagaacgg attcgccacc gaaacgctta acgcccaggc gtttagcttc
    14581 tgaatcgcga ccgttacgtg tggagccgcc agccttttta tgtgccattt gaaatctctc
    14641 ctcaggtctt aggcgctgat gccagtaatt ttcacatcag tgaaccactg acgatggccc
    14701 tgctgcttac gatagtgttt acgacgacga aacttaacga ttttaacttt ctcgccacga
    14761 ccgtgagcaa caacttcagc tttgattacg ccgccatcaa cgaaaggaac gccgattttg
    14821 acttcttcac cgtttgcgat catcagcact tcagcgaact caacagtttc gccagttgcg
    14881 atgtccagct tttccaggcg aacggtctga ccttcgctta ctcggtgttg tttaccacca
    14941 ctttggaaaa ccgcgtacat ataaaactcc gcttccgcgc acaccttttc aatgattcag
    15001 agtgcgctat aaatattcac aatagggcgc gaatattacg caaaacgcac gcctttgaca
    15061 agtgctacag tcaatacacg aagaaaaaaa acacaacttg tacggtaacg tttatctgtg
    15121 ccattttttc agtacaatca ccctatattc ctaaccataa accctaagtt gcctttgttc
    15181 acagtaaggt aatcggggcg aaaagcccgg cttttgcgat gaatttagaa aaaatcaatg
    15241 agttaaccgc gcaagatatg gcgggtgtta atgcggcaat ccttgagcag cttaattccg
    15301 acgtccaact gatcaatcag ttaggctatt acatcgtcag cggcggcggt aaacgtattc
    15361 gtccgatgat tgctgtactg gctgcacgag ctgttggcta tgagggaaat gcgcatgtca
    15421 ccattgctgc cctgatcgag tttatccaca cggcgactct gctacacgac gacgttgtgg
    15481 atgaatcaga tatgcgcagg ggtaaagcta ccgccaacgc cgcatttggc aatgccgcca
    15541 gcgtgctggt aggcgatttt atttataccc gcgcgttcca gatgatgacc agcctcggtt
    15601 cactcaaagt gctggaagtc atgtcagaag ctgtaaacgt catcgcagaa ggtgaagttc
    15661 tgcaactgat gaacgttaac gatccggaca tcactgaaga aaactacatg cgcgttatct
    15721 atagcaaaac cgcgcgtctg tttgaggctg ccgcgcagtg ttccgggatt ctggctggct
    15781 gtacgccgga ggaggagaaa ggcctgcagg attatgggcg ctatctcggc actgctttcc
    15841 agttgatcga cgatttactc gattacaatg ccgatggcga acagttaggt aaaaatgtcg
    15901 gcgacgatct gaacgaaggt aaaccgacgc tgccgctgct gcatgcgatg catcatggca
    15961 caccagaaca ggcacagatg atccgtaccg ccatcgaaca gggtaacggt cgccatcttc
    16021 tggaaccggt tctggaagca atgaacgctt gtggatctct tgaatggacg cgtcagcgtg
    16081 ccgaggaaga agcagacaaa gccatcgcag cattacaggt gctcccggac accccttggc
    16141 gagaagcact catcggcctc gcgcacatcg ctgttcaacg cgatcgttaa tcccctccct
    16201 tcatcccgcg cagtgcgcgg gatgattcca gtaaattcca taaaaacact aattcagcgc
    16261 tataacacct gaaaaaaagg gcgatgaatc tttatatatt ctgaatattc acactcttta
    16321 caggaacttt ttagagcaat aggccatcag gagtatagtg atgcccgaca gaagaagtgt
    16381 tctgaatgaa agcgactaac ttaaggagtg aggaaagatg gaaagtaatt tcattgactg
    16441 gcatcccgct gacatcattg cgggtttgcg caagaaggga acgtcaatgg cggcggagtc
    16501 tcgcagaaat ggtttgagtt cctcaacgct ggcaaacgca ttatcgcgcc catggccgaa
    16561 aggagagatg attattgcga aagccctggg aactgacccc tgggttatct ggccatcacg
    16621 ctaccatgat ccgcagaccc atgagtttat cgacagaacg cagttgatgc gtagctacac
    16681 taaaccgaaa aaatgagtgg tctggcggta gccccgcgaa cggggctgcc agctctcaga
    16741 cgattactcg cctttcacac gctcaatatt tgcacctaaa gcgcgcagtt tgtcttcaat
    16801 gcgttcgtag ccacgatcga tgtgataaat acgatcaacc accgtcgtcc cttccgcaat
    16861 acagccagcc agcaccaggc ttgctgatgc acgcagatcg gttgccataa cctgtgcgcc
    16921 agaaagtttt tcaacaccgt gacaaataac ggtattgctt tcgatttcgg catgcacgcc
    16981 catacggcta agctccggca catgcataaa gcggttttca aagaccgttt cggtgatgaa
    17041 tccggtccct tctgccacca ggttcaacag cgtgaactgg gcctgcatat cggtcgggaa
    17101 tgccggatgc ggcgcggtac gtacgttaac agccttcgga cgtttgccat gcatatccag
    17161 gctaatccag tcttcgccga cttcgatgtc cgctccagcg tcacgcagtt tcgccagcac
    17221 ggcgtcgaga gtatctggct gcgcgttacg gcagataatt ttgccgcgag aaatcgccgc
    17281 cgccaccagg aaagtaccgg tttcgatacg atccggtagc acgcgataga caccgccgcc
    17341 taaacgttcc acaccttcga tgacgatacg atcggtgccc tgaccgctaa ttttcgcacc
    17401 cagcgtaatc aggaagttcg cggtatcgac gatttccggt tcacgcgctg cgttttcaat
    17461 aatcgtggta ccttccgcca gggttgcagc acacatgatg gtcaccgttg cgccaacgct
    17521 gactttatcc atcacgatat gtgcgccttt cagacgacca tcgacggaag ctttaacgta
    17581 accttcttcc agtttgatgg tcgcgcctaa ttgttcgagg ccagaaatgt gtagatcaac
    17641 cggacgcgca ccgatcgtac aaccgccagg cagtgaaact tgcccctgac caaagcgcgc
    17701 taccagcggc cccagtgccc agatagaagc acgcatggtt ttaaccagat cgtaaggtgc
    17761 gcagaataca ttaacgtcgc gggcatcaat atgcacagaa ccattacgtt ctactttcgc
    17821 acccagctgg cttagcagct tcattgatgt atcgacgtct ttcagttttg ggacgttctg
    17881 gatctctacc ggttcttccg ccagtagagc ggcaaaaagg ataggcagag cagcattttt
    17941 agcgccggaa attgtgactt cgccctggag cttcgttggc ccctgaacac gaaatttatc
    18001 catttagttt gttctcagtt aacaattcat atccgctacc ggcgaatcgc ccatagctca
    18061 aaagccgttc agtttgcgat cgcgcgccca ctccgcaggg gtatacgctt tgatcgacac
    18121 agcatgaatg cggttatccg caatatattc catcagcggg ccatagaccg tctgctgttt
    18181 tttaacccga ctcatgccgt caaacaactc acccacggca ataacctgaa agtggctgcc
    18241 atcgccggaa acgtggactt cctggaggga gagagcgttc atcaacacgc tctgaatttc
    18301 attattttcc atgggatctt caatcatcag ttaataaacc agcgaaacat cttagagcaa
    18361 agttgcgctg gcataaataa gcaaaaagcc tcgctgataa atcagacaag gctcgacttg
    18421 caggcaggtt tgccggacag gcggttaacg ccatatccgg cctgaaaaaa tttaacgagg
    18481 cagaacatca gcaggcaaat tatacaattt cgcgagggta tacactttgt cgtttacccc
    18541 ctgaagcgtc acgttgttgc cctgcttttt cgccagatcg ataagatgga gcagcagtgc
    18601 cagtccaccc gtatccacgc gggacacacg gctaagatcg atgcaggtaa tccccttcac
    18661 cgcttcctca cgcatttccc aaagcggtag cagaacgtcc tgatccagct ctccggataa
    18721 cgccagcgtg tcacccgtct gcatccagct cagtgactcg ctcattattt tttctcttcc
    18781 agagtgattt tctgttgaga aatcgatttc agttgcgcag tcaggccgtc gatacctttg
    18841 gtacgcagca gcgttcccca ctcgttttgt ttggtggtga tcatactgac gccttcagca
    18901 atcatgtcgt aagcctgcca gtttcccgtc tgggagtttt tacgccactg gaagtccaga
    18961 cgcaccggcg gacggccatt cgggtcaata atggtaacgc gaataggcac aatggtttta
    19021 tcgcccagcg gctgttctgg cgcaatctga taggtttgac cgtgatacat cgccagcgcc
    19081 tgaccgtaag cctgcttcag gtactcacgg aaagcggcaa agtaggcttc acgttgagca
    19141 ggggtcgcac tcttgtaata ctggcccagc accagcgcac cggcgtattt cacctgtacg
    19201 tatggcagca gttcctgatc aacaatggta cgcagataat ccgggttggc ccgaatttgc
    19261 ggttgctcat tcttcaggcg atcgaacgtt ttctgcgccg cctcgtccat cagcttataa
    19321 ggattggtct ggtctgccgc ggttgccgca ctcagaggtg caatcaccag caaagcgacc
    19381 atcattaaac gtttaaacat gcgtcggttc tcctgaaatt atttcgttgt acccacaggt
    19441 tcagtggttt cattattacc tggcgcagca gctggcgcat cgccactatt cttattgtca
    19501 tcgcctttac taccgtaaag gaactgacca atgagatctt ccagcaccat cgcagactta
    19561 gtgtcctgaa ttgtatcgcc atccttcagg atagcagtcc ccagttccgg gtcttcaaaa
    19621 ccgacgttta atgccagata ttgttccccc agcaggccgg aagtacgaat gctcagcgaa
    19681 ctggtatctg gaatgtggtt ataacgttgt tcaatttcca gcgttacgcg cggcagatag
    19741 gttttcgggt ccagcgtaat atccgccacc cgacccacaa caacgccacc aatactgacc
    19801 ggagagcgcg ctttcaggcc gccaatgtta tcgaacgtcg cataaagcgt gtaggtcggt
    19861 tcagtacgta tggacgtcac gttcgccgcc ttcaggcaaa caaacagcgc cgccagcagt
    19921 gctgctaata aaaagatacc cacccaaatt tcattttttt tcgtttgcat gaactcaatt
    19981 cccaaacatc aatgcggtca gcacaaaatc cagccccaga acagccagag acgagtggac
    20041 aacggtgcga gtggttgccc ggctaatccc ggcagacgtc gggatggcgt cgtagccgtt
    20101 aaacaacgaa atccacgtca ccgtgatggc gaacaccacg ctcttaatca gacagttgac
    20161 cagatccata cgccagtcga cggcattttg cattgccgac cagaagaacc cgctatcaat
    20221 gcctttccaa ctgacgccga ccagagatcc gccccagatc cccacggcga cgaaaataac
    20281 cgtcaacagt ggtaatgaaa taaccccagc ccagaaacgg ggagaaataa cccgacgcag
    20341 cggatccacc gccatcatct ccatactgga gagttgctct gtagcgcgca tcaggccgat
    20401 ttctgcggtt agcgccgaac cagcacgccc ggcaaacaac aacgcggcaa ccaccggccc
    20461 cagttcacgc agtagcgata acgccaccag catacccaga ctggtttccg cactataagt
    20521 ggtcagaacc agataacctt gcagccccaa caccattccg atgaacacgc cagaaaccac
    20581 aataatcagc atcgacagga cgccgacatt atagagctgg cgcaccagca gcggcgcatg
    20641 tttgcgaaat tccggtttgc cgaccagcgc attgaataac attaacccgg cccgcccgaa
    20701 cgttctcagg gttttaatcc ctttatgtcc gagcgacgcc agcgcattta acagcatgag
    20761 tggcttaact ccctggtaaa agatcagcgt gataatcgcc ggcaggatag cggaacggaa
    20821 caggcccgtc agctatcccg tccagaaact gacgtacgcg cggatcagga ttcgcctgca
    20881 acgcctgggc actgccatga gcgacaattt ttttgtccgc caggatccag gcgtgatccg
    20941 caatacttaa cacttccggc acatcgtgag aaaccaccac acaagtcacg cccagcgcgc
    21001 tgttcagctc agaaatcagc ttcaccagta cgcccatggt gatgggatct tgcccaacaa
    21061 aaggttcatc aaacatgatg agatccggct ccagcgcaat cgcacgcgcc agcgctgcac
    21121 gccgcgccat cccaccggaa agttcagaag gcattagttt agccgctcca cgcagcccca
    21181 cggcctccag cttcatcatc accgtactat gcaacaatgg cgcgggtagt tgggtatgtt
    21241 cgcgcagtgg ataggcgacg ttgtcaaata cgttcatatc agtgaacaac gccccggact
    21301 gaaataacat gctcatccgt ttgcgcactg tatacaggcg cgaacgagac atcgccggaa
    21361 tattctcacc atcgaaaagg atctcaccat gatctggtgc gatttgcccg ccaatcagac
    21421 ggagtagcgt cgttttaccg atgcccgatg gccccatgat cgccgtgatc ttccctcgcg
    21481 gcacggtcag ggaaatatta tcgaagatgc agcgattgcc acgcgtaaaa ctgacatcgc
    21541 gcatatcgac taaattcgcc acagactgct ccataattca cccttcgtct tgcgttgatt
    21601 ttctaagcat ggcgctcaat ttaaccttga acccaacata tttacagaat attacccgcc
    21661 gtggttagcg aaagctggca tttgttttac tttttagccg cataaagtca aaattaagaa
    21721 tccgttacgg ctttctgaaa atcttcagcg gaccgacgag tatacctgaa gaaaggacgt
    21781 tagatgcttt tagctacggc actgttaatt gttggtttac ttttggtcgt ttacagtgcc
    21841 gaccgcctgg tttttgccgc gtctattctt tgccgaacct ttggcatccc gccgctgatc
    21901 atcggcatga cggtggtcag tattggtaca tcgttaccag aaatcatcgt ctcgcttgcc
    21961 gcgtctctgc acgaacaacg cgatttagcc gttggtacag ccctcggctc aaacattatc
    22021 aatattttgc tgatcctcgg tctggctgcg ctggttcgtc cttttaccgt ccattccgat
    22081 gttctgcgtc gtgaattacc cttaatgttg ttggtcagcg tagtggccgg ttccgtactc
    22141 tatgacggac aacttagtcg cagcgatggt atctttctcc tctttctggc tgtgctatgg
    22201 ctgctgttca ttgttaaact tgcacgtcag gctgaacgtc aggggactga cagcctgacc
    22261 agagagcagc ttgcagagct gccgcgtgac ggcggattgc ccgtcgcgtt tttatggctc
    22321 ggcattgcgc ttatcatcat gccagtggcc acgcggatgg tggttgataa cgccacggtg
    22381 ctggcgaatt actttgccat cagcgagttg acgatgggtc tgacggcaat tgctatcgga
    22441 accagcctgc cggaactggc aaccgcaata gcgggggttc gcaaaggtga aaacgacatt
    22501 gctgtcggaa atatcattgg cgcaaacatt tttaatattg tcatcgtgtt gggtttaccc
    22561 gcgctgataa cgccaggaga gattgagcca ttggcgtaca gtcgtgacta cagcgtgatg
    22621 ttgctggtga gcattatttt tgcgttgctg tgctggcggc gctccccgca accgggccgt
    22681 ggtgtagggg tattattaac tggcggattt atcgtatggc tggcgatgtt gtactggtta
    22741 tcgccaatac tcgttgaata actggaaacg cattatgtcg cacgtagagt tacaaccggg
    22801 ttttgacttt cagcaagcag gtaaagaagt cctggcgatt gaacgtgaat gcctggcgga
    22861 gcttgatcaa tacatcaatc agaatttcac gcttgcctgt gaaaagatgt tctggtgtaa
    22921 agggaaagtt gtcgtcatgg ggatggggaa atcggggcac atcgggcgca aaatggccgc
    22981 aacgtttgcc agcaccggta caccttcatt tttcgtccat cctggtgaag ccgcgcatgg
    23041 tgatttaggc atggtcaccc cacaggatgt ggtgattgct atctctaact caggtgaatc
    23101 cagcgaaatc acggccttaa ttccagtgct taagcgtctt cacgtaccgt taatctgcat
    23161 caccggtcgc ccggagagca gcatggcgcg cgccgcagat gtgcatctgt gtgttaaagt
    23221 agcgaaagaa gcctgtccgt tagggctggc accgaccagc agcaccaccg ccacgctggt
    23281 tatgggcgat gccctcgctg tcgcgctgtt aaaagcacgc ggctttactg ctgaagattt
    23341 tgcgctctca cacccaggcg gcgcactggg tcgtaaactt ctgctgcgcg taaacgatat
    23401 tatgcatacg ggcgatgaga tcccgcatgt taagaaaacg gccagtctgc gtgacgcgtt
    23461 gctggaagtt acccgcaaaa atcttggtat gactgtcatt tgcgatgaca atatgatgat
    23521 tgaaggcatc tttaccgacg gtgatttacg ccgtgtcttc gatatgggcg tggatgttcg
    23581 tcagttaagt attgccgatg tgatgacgcc ggggggaata cgtgtgcgcc ctggcattct
    23641 ggccgttgag gcactgaact taatgcagtc ccgccatatc acctccgtga tggttgccga
    23701 tggcgaccat ttactcggag tgttacatat gcatgattta ctgcgtgcag gcgtagtgta
    23761 aagattcaag gataaacaac aatgagcaaa gcaggtgcgt cgcttgcgac ctgttacggc
    23821 cctgtcagcg ccgacgttat agcaaaagca gagaacattc gtctgctgat cctcgatgtc
    23881 gatggcgtac tgtcagatgg cctgatttat atgggcaata atggcgaaga gctgaaagcg
    23941 ttcaatgttc gtgacggtta tggcattcgt tgtgcgctca cctctgatat tgaagtcgct
    24001 atcattaccg ggcgaaaggc taaactggta gaagatcgtt gtgccacatt ggggatcact
    24061 cacttgtatc aggggcagtc aaacaaactg atcgccttta gcgatctgct ggaaaaactg
    24121 gcgattgccc cggaaaatgt ggcttatgtc ggcgatgatc tcatcgactg gccggtaatg
    24181 gaaaaagtgg gtttaagcgt cgccgtggcc gatgcgcatc cactgttgat cccgcgcgcc
    24241 aattacgtga cgcgcattgc tggcggtcgt ggcgcagtgc gcgaagtttg cgacttatta
    24301 ctcctggcgc agggcaaact ggatgaagcc aaagggcaat cgatatgagt aaagccagac
    24361 gttgggttat cattgtgcta tcactggcgg ttctggtgat gatcggcatt gatatggccg
    24421 aaaaagacga taccgcccag gtggtcgtca acaacaatga tcccacctat aaaagcgagc
    24481 atacggacac gctcgtctat aacccagaag gggcactaag ctatcgattg attgctcaac
    24541 acgttgaata ttattccgat caggccgttt cgtggtttac gcagccggta cttaccacgt
    24601 ttgataagga taaaatcccg acatggtccg taaaagcaga taaagccaag ctgaccaatg
    24661 accggatgct ctatttatat ggacacgttg aagtcaacgc acttgtgcca gactctcaac
    24721 ttcgcagaat cacgacggat aacgcgcaga tcaatctggt gacgcaggat gttacctctg
    24781 aagacctcgt cacgttatac ggaacaacat ttaactccag cggtctgaaa atgcgcggca
    24841 acttacgcag caagaacgcc gagctgattg aaaaggttag aacatcctat gaaattcaaa
    24901 acaaacaaac tcagccttaa tcttgtgctt gccagctcac ttctggccgc cagcattccg
    24961 gcatttgccg taaccggaga cactgatcag ccgatccaca ttgaatcgga ccagcaatct
    25021 cttgatatgc aaggcaacgt ggttaccttt accggtaatg tcatcgtcac ccagggcacc
    25081 atcaaaatta atgccgacaa agtggtcgtt acccgtccgg gcggcgaaca aggtaaagaa
    25141 gtgattgacg gctacggtaa accggcaacg ttctaccaga tgcaggacaa cggtaaaccc
    25201 gttgaaggtc acgcttccca gatgcactac gaactggcaa aagattttgt cgttctgacg
    25261 ggtaatgctt atctgcagca ggtcgatagc aacattaagg gcgataagat cacttacctg
    25321 gtaaaagagc agaaaatgca ggctttcagc gacaaaggca agcgcgtaac caccgttctg
    25381 gtgccgtcgc agctgcagga caaaaacaac aaaggccaga ccccggcaca gaagaagggt
    25441 aattaattcg ttatggcaac attaactgca aagaaccttg caaaagccta taaaggccgt
    25501 cgcgtggtag aagacgtcag cctgaccgtc aactccgggg aaattgtcgg tctgctgggg
    25561 ccaaacggtg ccggtaagac caccactttc tacatggttg taggcattgt gccgcgcgat
    25621 gcgggcaaca tcattattga tgatgacgat atcagtctgc tgcctctgca tgcacgcgcg
    25681 cgccgcggta tcggctatct gccacaggaa gcctccattt tccgtcgcct cagcgtttac
    25741 gataacctga tggcggtact gcaaattcgt gacgacttgt ctgctgaaca acgtgaagac
    25801 cgcgcgaacg agctgatgga agagtttcac attgagcacc tgcgtgacag catggggcag
    25861 tcactctccg ggggtgaacg tcgccgtgta gaaattgccc gcgcactggc tgcgaatccg
    25921 aaatttattc tgctcgacga accgtttgcc ggggttgacc cgatctcggt tatcgacatt
    25981 aaacgcatca ttgagcacct gcgcgacagc ggcctgggcg tgctgatcac tgaccacaac
    26041 gtgcgtgaaa cactggcggt ttgtgaacgc gcttatatcg tcagtcaggg gcatttgatc
    26101 gcccacggca cgcctacaga aatcttacaa gacgaacacg ttaagcgtgt ataccttggg
    26161 gaagacttca gactctgata gggtagaagt ttgcgacgtt ttagcaggag agtacgattc
    26221 tgaacatgaa gcaaggtttg caactcaggc ttagccaaca actggcgatg acgccacagc
    26281 tccaacaggc aattcgtctg ttgcagttgt cgacgctgga acttcagcag gagctacagc
    26341 aggcgctgga gagtaatccg ctgcttgagc aaatcgacac tcatgaagaa atcgacaccc
    26401 gcgaaacgca agacagtgaa acgctggaca ccgccgacgc gctcgaacaa aaagagatgc
    26461 cggaagagct gccgctcgat gccagttggg acaccattta caccgctggt acaccatccg
    26521 gcaccagcgg tgactacatt gacgatgagc tgccggtcta tcagggcgaa acgacgcaga
    26581 ccttgcagga ttacctgatg tggcaggtcg agctgacacc gttttccgac actgaccgcg
    26641 ctattgctac ctctatcgtc gatgccgttg atgacatcgg ttatctgact gtcccgctgg
    26701 aagatattct cgaaagtatg ggcgatgaag agatcgacat cgacgaggtt gaagccgtcc
    26761 ttaagcggat ccaacggttt gatccggtcg gtgtagcggc aaaagatctg cgtgactgcc
    26821 tgctaatcca actctcccaa ttcgataaaa ccacgccgtg gctggaagag gccagactga
    26881 tcattagcga tcatctcgat ctgttagcca atcacgactt ccgcacttta atgcgcgtca
    26941 cgcgtctgaa agaagatgtg ctgaaagaag ccgtcaatct gatccagtcg ctcgatccgc
    27001 gccccgggca gtcgatccag actggcgaac ctgagtatgt cattccagat gtgctggtgc
    27061 gtaagcataa cggtcactgg acggtagaac tcaacagtga cagcattccg cgtctgcaaa
    27121 tcaaccagca ctacgcctcg atgtgcaata acgcgcgcaa cgatggtgac agccagttta
    27181 tccgcagcaa tctgcaggat gccaaatggt tgatcaagag tctggaaagc cgtaacgata
    27241 cgctactgcg cgtgagtcgc tgtatcgttg aacagcagca agccttcttt gagcaaggtg
    27301 aagaatatat gaaaccgatg gtactggccg atatcgccca ggctgtcgaa atgcatgaat
    27361 cgacgatatc tcgcgtgacc acgcaaaaat acctgcatag tccacgaggc atttttgaac
    27421 tgaagtattt cttttccagt cacatcaata ccgagggcgg cggcgaagct tcctccacgg
    27481 cgattcgtgc gctggtgaag aaattaatcg cggcggaaaa cccagcgaaa ccgttgagcg
    27541 acagcaagtt aacctctttg ctgtcggaac aaggtatcat ggtggcacgc cgcactgttg
    27601 cgaagtaccg agagtcttta tccattccgc cgtcaaacca gcgtaaacag ctcgtttgac
    27661 ccaaccgata aggaagacac tatgcagctc aacattaccg gaaataacgt cgagatcacc
    27721 gaggcactgc gcgaatttgt tacagccaaa tttgccaaac ttgagcaata ttttgaccga
    27781 atcaaccagg tctatgttgt tctgaaagtg gagaaagtca cccacacctc agatgcaaca
    27841 ctgcatgtaa acggtggtga aattcatgcc agcgcggaag gtcaggacat gtacgctgcc
    27901 attgatggct taattgataa gctggcacgc cagttgacca aacataaaga taaactgaaa
    27961 caacactaat tgtccgggca attagcatgt gcatggcggc ctgttgtgcg gcacaacggg
    28021 ccatttgtac ggttaatgct ccgagcctgt tccactgttt gagtgggcag gttcttaggt
    28081 gaaattatga caaataatga tacaactcta cagcttagca gtgttcttaa cagggaatgt
    28141 acgcgaagcc gcgtccactg tcagagcaaa aaacgcgccc tggaaatcat cagcgagctg
    28201 gcggcgaaac aacttagcct gccgcctcag gtggtttttg aagctatcct gacgcgggaa
    28261 aaaatgggca gtaccggtat cggcaatggt attgccattc cgcatggcaa actggaagaa
    28321 gatactctgc gcgccgttgg cgttttcgtt cagctcgaaa cgcctatagc tttcgatgcc
    28381 atcgacaacc aaccggtgga cttacttttt gccctgctgg tgtcggcaga ccaaactaaa
    28441 acgcacctgc atactctgtc gctggtggcg aaacgtctgg cggacaaaac catctgccgc
    28501 cgtttacgcg cagcccagag cgatgaagag ctgtatcaaa tcattacgga taccgaaggt
    28561 actccggatg aagcgtagtt attcggtaat gtctctttta gacgttgtga ggagaaacag
    28621 tacatggtac tgatgatcgt cagcggacgt tcaggttcag gtaaatctgt cgccctgcgt
    28681 gcgctggaag atatgggttt ttactgcgtg gataaccttc ccgtagtgtt gttacccgat
    28741 ctggctcgaa ccctggccga tcgcgagatt tctgccgccg tcagcattga tgttcgtaat
    28801 atgccggagt caccagaaat attcgaacag gcgatgagta acctgcctga cgctttctca
    28861 ccgcaactcc tgttcctgga tgccgaccgt aataccttaa ttcgtcgtta cagtgacacg
    28921 cgccgactgc atccgctttc cagcaaaaac ctgtcgctgg aaagtgctat cgacaaagaa
    28981 agcgatttgc tggagcctct gcgttcgcga gcggatctga ttgtcgatac ctcagaaatg
    29041 tccgttcacg agctggctga aatgctgcgt acccgtctgc tgggtaaacg tgaacgcgaa
    29101 ctgaccatgg tctttgagtc tttcggcttc aaacacggta tccctatcga tgcagattac
    29161 gtctttgacg tgcgcttctt gccgaacccg cactgggatc cgaaactgcg tccaatgaca
    29221 ggtcttgata aacctgtcgc cgcgttcctc gaccgccaca cagaagtaca caattttatc
    29281 taccagacgc gaagctatct tgagctatgg ttacctatgc tggaaaccaa caaccgtagc
    29341 tacttgacgg tcgccattgg ttgtaccggc gggaagcacc gttcggtgta tattgcagag
    29401 caactggcag actacttccg ctcgcgcggt aaaaacgtcc agtcacgcca tcgtacgctg
    29461 gaaaaacgta aaccatgacc gtcaagcaaa ctgttgaaat cacaaacaag ctgggcatgc
    29521 atgcccggcc tgcaatgaag ctgtttgaat taatgcaggg ttttgacgct gaagtgctgt
    29581 tacgtaatga cgaaggcacc gaggccgaag ccaacagcgt tattgcgctg ctgatgttgg
    29641 attctgccaa aggacggcag attgaagttg aagcgaccgg cccacaggaa gaggaagcac
    29701 tggccgccgt tatcgccctc tttaactctg gttttgatga agattaatct tcatcacttt
    29761 gacatacaac tcccttcaaa ctccccccga caataagaaa atcacgtact gaaatcgttc
    29821 tcaatcaacg tcatttgtac attttgtgcg cttttcactt tcagaagaac cttaagaaaa
    29881 ccttaagagg cattgtttag gttttgttta agttaatcga ccatactgga gatcgtcaga
    29941 aaatatttcc aggagatggc atgattcgct tatctgaaca aagtcccctg ggcaccggaa
    30001 gacatcggaa atgttatgcg catccggaag atgcccaacg ctgtatcaag attgtctacc
    30061 atcgtggcga tggtggcgat aaagagatcc gccgtgagtt aaagtactac gcgcatcttg
    30121 gtcgccggtt aaaagactgg agtggaatac cgcgctatca cggtaccgtt gagacggatt
    30181 gcggaaccgg ttatgtctac gatgtgattg cagattttga cggcaaactc tccattacgc
    30241 tgaccgaatt tgctgaacaa tgccgttacg aagaagacat tgcacaactg cgccagctac
    30301 tgaaacagct gaagcgttat ttgcaggata accgtatcgt gacgatgtcg ctgaagccgc
    30361 agaatatcct ctgtcaccgc atcagtgagt cagaggttat cccggtcgtc tgcgacaaca
    30421 ttggtgaaag cacattgatt ccactggcga cctggtcgaa gtggtgttgt ctgcgtaagc
    30481 aagagcggtt atggaaacga tttatcgccc aaccggcgct ggctattgcc ctgcagaaag
    30541 atctgcaacc tcgtgagagc aaaacgctgg ccctcacttc gcgcgaagct taatccagct
    30601 ggtgttgttg cataaacggc tcaccgccta actgatacat ctgccgtaaa atccacgcct
    30661 gacggctacg cacgtagccc gatggcgagg agactttgaa acgaagtgga ttaggtaata
    30721 cagctgccag taatgcagct tccgaccggg taagtttgct cgcgggtttg tggaaataac
    30781 gttgtgccgc agcttcgacg ccaaacacac cgtcgccaaa ttcggcgata ttcaggtaaa
    30841 ccgtcaggat acgctttttg ctccagactg tttctatccc cagcgttaat ccagcttcca
    30901 gcccttttcg tacccagcta cgcccatccc ataaaaagag atttttggcc gtctgctgag
    30961 aaatcgttga agcaccgcga atacggtttt cattgcgctc gttgtgcgcc agggctttct
    31021 caatggaagc gacatcaaag ccccagtgct caggaaattt ctgatcttct gcggcaatca
    31081 ccgccagtcc catccacggc gagatttgat ccatactgac ccagtcagaa tgtgccacgt
    31141 aacgaaaatt gccatgcagc caggcgctga cctgtcgctc gaccattacc gctgagaagg
    31201 gaacaggcgc aacactaaac aacgcgatgc ccccgcccca gaaaacggcg aggacaacca
    31261 ttaaccgcaa aagaaaacgg cgaacgaaac taaacaccgt taagcggctt ttactcattc
    31321 agccagaacc agcacgcggg aaaccagctt atcaatgccg ctcgccgctt ctgcaatgtt
    31381 ctgcgccagc atatatgctg gggtggtgac aatcttattg tcttcatcaa ccacgatatc
    31441 atcgacagga cagggcacat gctccgcgcc catctcttcc agcacttctg cggtatcgat
    31501 atcagtaccg atggtcaaac gcagcgggaa atcgaaaatt ttcggcagca tcgccggggc
    31561 aatacacata aaaccaagcg gttttccggc ctgatgcatc gcttgtgcca gcgcctttaa
    31621 ttcacggtca acggtgcatt cgctaccaag agtggcaaaa ttgcttaaat tcttcgccgc
    31681 gccaaacccc cccggcacaa tcaacgcatc cagttcagcg gcatcggcct gggccagagg
    31741 acggatttca ccgcgcgtta ttcgtgccgc ttcaatcagc acgttgcgcg tttccgtcat
    31801 cgcttcgcca gttaaatggt tgataacatc aacctgctgc ttatccggtg caaagcagac
    31861 cgcctgcgca ccgctgcgtg aaatagctaa caacgtcaac accgcttcat gaatttcaga
    31921 accgtcatag acgccgcatc cgctcagaat tacgccaatt ttcttcattg tgatcatcct
    31981 ttcgcaattg actgaaacac attaattttt ttaataaaaa tggtacgcat cacacattta
    32041 actgattcat gtaacaaatc atttaagttt tgctatctta actgcgtgcg gcctgaaaaa
    32101 cagtgctgtg cccttgtaac tcatcataat aatttacggc gcagccaaga tttccctggt
    32161 gttggcgcag tattcgcgca ccccggtcta gccggggtca ttttttagtg gcttttgcca
    32221 cccacgcttt cagcacttct acgtcgtgac gccactcttc tttcatctct tcaatccatt
    32281 caccgacgtt atcttcccag gcaggaaggt caggagactg aatttgctga cccagctgtt
    32341 gcagatggcg taaccccact gaccccgccg cacctttaat tttatgtcct tcctcaacaa
    32401 tgcctttttt atcctgcgcc gtcagattcg actccagcac gctgacatag cccggcatca
    32461 tcttctcaaa caccgctaac ccgtcggtga tcagcttcgg tcctacaagt tcgagatact
    32521 gttccagcat gggaatatcg agcaatgctt ctgatttact gttctcttct gtcgtcaccg
    32581 tactctcctc atcatcctgg gtatcccaga atttcttgat catcgcggtt agcgccggaa
    32641 cagaaagcgg cttactcagc acatcatcca ttccagcatt gaggtactct tgtttgtctt
    32701 tcagcacgtt agcggttaag gccaccagcg gcggtaaatc ctctcgcgga taacgcttcg
    32761 tcagttcacg agagatatcc agcccggtca tatctggcaa ctgaatatcc agtaacacca
    32821 ggtcgtattc gcccggttta aacatctcta gcgccgcctt gccggtcatg gcgacatcaa
    32881 cgctgttacc taatttttcc agcacagaac gcgcaacaat cacgttcagt tcaatgtctt
    32941 ccaccagcag cacattcagc gccggtaaag gcatatcgtc ttcatcaaac gcatcatcga
    33001 cctcttctgc taccgacggt gcgtggatcg tcaacgtaaa ggttgaacct ttgccctgtt
    33061 cgctggtaac cgtaatatcg ccgcccatat ttttcgccag acgacgagaa acggccagac
    33121 caataccggt gccggtggca ggtttaccgc catgactgtc tttcacctgg taatacatgg
    33181 cgaaaatttt atccagctca tcctgcggaa tgccgatacc agagtcttcc acttcaaaat
    33241 gcagcatatc gccttcatcg tagcgcacgc gcacggtaac ctggccttgc tgggtgaatt
    33301 tgacggcgtt actgatgagg ttccacagga tctgccgtaa acgcgtcccg tcggtaatga
    33361 cctgatgcgg taatggcagc gtcggctcca ggttaaagcg caatcctttt tgttgcgcct
    33421 gcaatgcgga gagattttcc agatcggcaa ggaagctggt gaaatcaacc ggttgattat
    33481 caagctggac cttgcgccgt tccatcttat ccatgtcgat aatatcgtta aagatattcc
    33541 ccagcgtgac ggccgaaaca tggatggtct tgagatattt ttcctgctcg gcggtgagtt
    33601 cggtatccag cagaatgcgg ctcagaccga cgataccgtt cagcggtgta cgcaattcgt
    33661 gactgatggt ggagataaac gtcgttttgt cgcggctggc ccgttcaagc gcatcctgat
    33721 accgcttacg ctcggtaatg tctcgaccaa agcccatcaa accgtgacgt ttacccacgc
    33781 ggtcgtagta cggcacttta cggatttcaa agcaggcttt gcgcccgtcc gggtaatcca
    33841 gccactgttc ataggtcagt gacacattat gacggaacac tttttcatcg gtttcaatga
    33901 cttttgcggc ggcttccggt gagtaaacat cagcaggttt caggtgaacc agttgttttt
    33961 cgctttttcc ggtcagcagc tccatcgcgc ggttacagcc ggaaaactct ttatcttcgt
    34021 tacgataaaa aaccaggtcg ggtgaagcat caaggaagga acgtaagaat gaggattgct
    34081 gctcgagctg aatttgtgtc tcttcgcgct ctttgatttc aattttcagt tggccgaagg
    34141 tttcctgcag ttctgcttcc gctttttcac gaacggcgat ttcctgattt agctgggcaa
    34201 tattatcttt taactgaacg ttgaggctca aatcgcgctc gcgcatctcc tccagttttt
    34261 gcaccagccg tgacagacgt tgtcgtgact cctccagttg ctcgacgacc accgatagaa
    34321 agtagaccgc ccacggcgta atcaacaaac caaagaagat agaacgaata acatcaatgc
    34381 tttcgacctg accatgcagc accatggtta ccgccatttg taccacaatg gcaagaacga
    34441 cgagggccag cgccagcaac attgagaagc gcaccagacc taacttcatc atcaggtcaa
    34501 cataatactg cgccagcaga cgaatttgct tcattaggga attccttcac gacaacctgc
    34561 cccaataata cccaattctg acagttacgt tataaattgt gtgagaaatg cggaattccc
    34621 ctcaacgacc tactccgttg gaggtagcca cggacgtccc agcgccgatc cctgtacgcc
    34681 atgttcattc agatagcgat ccagctcgac cattcccgtc catcgatttt cgcaccacag
    34741 aggagcaagc agcgtcgggc gacgggcgct ggcggaaatg cggtggtaga tcacttccgg
    34801 tggcgtatgg cgaatcattt ctccggcagt gagcgtgtaa tcctccagtt caataccgtt
    34861 caaacgcccc gcttcccagg ctttcgccat aatgctgcct ttcacaatat gcaacggatg
    34921 tagctttata ccgtcaacac cggtttccac tacccgctcc agcgtttgca gacattcagc
    34981 cagcccttcg cctggcagac cgacaattaa atgcgagcac accttcagcc cgcgctggcg
    35041 tgccagctgc gtcgtgcgct gataacaggc gaaatcatga ccgcgattaa tgcgatgtag
    35101 cgttttgtcg tgcacggttt gcagccccag ctccagccac acttcgtagc cctggtcttt
    35161 atattcgcaa agcagatcca gcaccgcatc cggcacgcag tccgggcgcg tgccgacaca
    35221 caaaccgaca atattggcct ggctcaccgc ctgttgatac atcgaacgca gcacctgaac
    35281 ttcggcaaag gtgctggtat acgcctggaa ataggcgagg taacgtttcg cgcgattaat
    35341 taaattcgcc tgatgcgcca gctgttcggc aatggaacga tgctgctgcg cttcgtcggc
    35401 aaacgaggca acattacaga aggtgcagcc gccgcgcccg atggtgccgt cgcgattcgg
    35461 gcaactgaaa ccgccgtgca gcgtcagctt atgcaccttt tgcccataac gacgggtgag
    35521 atcaccacca aacatattga ctaatttctg taactgcata atctgataga ccgcgccttg
    35581 aaaagaggcc aaagcctgcc atttttagct caattcggcg atgacctgga tcaatcgtcc
    35641 cggcccgctt ttatcaactg cataatcaat caaaattacc gaaatttcat gcataatcac
    35701 ataaatcact tttgcttatc ttgtgtcaga tttttttatc tccttatgga ttttaggcaa
    35761 aaacagtagc atgaaacgtc attaccaatt aatgcagtat aaaatgctgg ttttctcgtc
    35821 aattcaaggc aggataaggg ttaacacgcc tttatgacag tcaggaattg actgtttctc
    35881 taacgacttc ccttttagcc ttaaagataa aatctatttt aatttcagcc atttaataaa
    35941 gaattttgcg ctaaagcaca tttctgtacc aataagcttg ccatttgacc tgtatcagct
    36001 ttcccgataa gttggaaatc cgctggaagc tttctggatg agcagcctgc tcatcatatt
    36061 tatgcagtaa ttgagatccc ctcttcaccg tattaaccga tgcgaaaagg acaacaaggg
    36121 ggcgaatgcg aggcgcgcgt atgacacgca acccccgtcg ccacgctctt tctgtacccg
    36181 tgcgcagcgg ttcggtagtg gggttcccgc agagcctggg ggaggttcac gatatgttgt
    36241 acgataaatc ccttgagagg gataactgtg gtttcggcct gatcgcccac atagaaggcg
    36301 aacctagcca caaggtagtg cgtactgcaa tacacgcact ggcccgcatg cagcaccgtg
    36361 gcgcgattct cgccgatggt aaaaccggtg acggttgcgg cttgctgtta caaaaaccgg
    36421 atcgcttttt tcgcatcgtt gcgcaggagc gcggctggcg tttagcgaaa aactacgccg
    36481 tcgggatgct attcctgaat aaagatcctg aactcgccgc tgccgcacgc cgcatcgttg
    36541 aagaagaact gcaacgcgaa accttgtcga ttgtgggctg gcgtgatgtc cccactaacg
    36601 aaggcgtact gggtgaaatc gccctctcct ctctgccccg cattgagcaa atttttgtga
    36661 acgccccggc aggctggcgt ccgcgcgata tggagcgccg tctgtttatc gcccgccgcc
    36721 gcattgaaaa gcgtctcgaa gccgacaaag acttctacgt ctgtagccta tcgaatctgg
    36781 tgaacatcta taaaggtctg tgtatgccgg cggatctgcc gcgcttttat ctggatcttg
    36841 cggacctgcg tctggaatcg gccatttgcc tgttccacca gcgcttctcc actaacaccg
    36901 taccgcgctg gccgctggcg caaccgttcc gctatctggc gcataacggt gaaatcaaca
    36961 ccatcactgg taaccgccaa tgggcgcgtg cgcgtaccta taaattccag acgccgctta
    37021 tccctgatct gcacgacgcc gcaccgttcg tcaacgaaac cggctctgac tccagttcga
    37081 tggataacat gctggaactc ctgctggcag gcgggatgga tatcatccgc gccatgcgtc
    37141 tgttagtgcc acccgcctgg cagaacaacc cggatatgga cccggaactg cgtgccttct
    37201 ttgactttaa ctccatgcat atggagccgt gggatggccc ggcgggcatc gtgatgtccg
    37261 acggtcgttt tgccgcctgt aacctcgacc gtaacggtct gcgtccggcg cgctacgtca
    37321 tcaccaaaga taagctcatc acctgcgcct ctgaagtcgg tatctgggat taccagcctg
    37381 acgaagtggt cgaaaaaggc cgcgtcgggc caggcgaact gatggttatc gacacccgca
    37441 gtgggcatat tctgcactcg gcagaaaccg atgacgatct gaaaagccgc catccatata
    37501 aagagtggat ggagaaaaac gtccgccgac tggtaccgtt tgaagatctg cccgatgaag
    37561 aagtgggtag ccgcgaactg gacgacgaca cgcttgccag ctaccagaaa cagtttaact
    37621 acagcgcgga agagctggac tccgtaattc gcgtactggg cgaaaacggt caggaagcgg
    37681 tcggttcgat gggcgatgat accccattcg ccgtgctctc cagtcagccg cgcattattt
    37741 acgactactt ccgccagcag tttgcccagg tgactaaccc gccaatcgac ccgctgcgtg
    37801 aagcgcatgt tatgtcgctc gccaccagta tcggtcgtga aatgaacgtc ttttgcgaag
    37861 cagagggcca ggcgcaccgt ttaagcttta aatcgccgat tctgctctac tccgatttca
    37921 aacagctcac gacgatgaaa gaggagcact accgcgcaga tacgctggat atcacctttg
    37981 acgtcactaa aaccacgctc gaagcgacag tcaaagagct gtgcgacaaa gccgaaaaaa
    38041 tggtacgtag cggcaccgtg ctgctggtgc tctccgaccg gaatatcgct aaagatcgcc
    38101 tgccggttcc agccccgatg gcggttggcg cgatccagac ccgtctggtc gatcaaagcc
    38161 tgcgttgcga tgccaacatc atcgtcgaaa ccgccagcgc ccgcgatccg caccacttcg
    38221 ccgtgttgct gggcttcggc gcgacggcta tttatccata ccttgcctat gaaacgctgg
    38281 gccgcctggt agacacccat gcgattgcca aagattatcg taccgtgatg ctcaactacc
    38341 gtaacggcat caacaaaggc ttgtacaaaa tcatgtccaa aatgggcatc tccaccatcg
    38401 cctcttaccg ctgctcgaaa ctgtttgaag cggtcggtct acacgatgat gtagtgggcc
    38461 tgtgcttcca gggggcggtc agccgcattg gtggggcaag ctttgaagac ttccagcagg
    38521 atctgctgaa tctgtcgaaa cgtgcctggc tggcgcgtaa gcccatcagc cagggcggcc
    38581 tgctgaaata cgtccacggc ggcgaatacc acgcctacaa cccggacgtg gtgcgcacgc
    38641 tgcaacaagc ggtacaaagc ggcgagtaca gcgactatca ggaatacgcg aagctggtta
    38701 atgagcgtcc ggcaaccacg ctgcgcgatc tgctggcaat tacgccgggt gaaaacgagg
    38761 tcaacattgc tgatgttgaa ccggcaagcg aactgtttaa acgcttcgat accgctgcga
    38821 tgtctatcgg cgcgttaagc ccggaagccc acgaggcgct ggcggaagcg atgaacagca
    38881 tcggcggtaa ctcaaactcc ggtgaaggcg gtgaagatcc ggcgcgctac ggcaccaaca
    38941 aagtgtcgcg catcaagcag gtggcttccg gtcgctttgg ggttactccg gcgtatctgg
    39001 tcaatgccga cgtcattcag attaaagtcg cccagggcgc gaagccgggc gaaggcggtc
    39061 agttgccggg tgataaagtc actccttaca tcgccaaact gcgctattcg gtgcccggag
    39121 tgacgctgat ctccccgccg ccgcaccacg atatctactc tatcgaggac ttagcgcagc
    39181 tcattttcga cctcaagcag gttaacccga aagcgatgat ctccgtgaag ctggtttccg
    39241 aaccgggcgt aggcaccatc gcgactggcg tggcaaaagc ttatgccgat ttaatcacca
    39301 tcgcaggcta tgacggtggc accggcgcaa gcccgctgtc gtcagtgaaa tacgcaggtt
    39361 gcccgtggga gctggggctt gttgaaaccc agcaggcgct ggttgccaat ggtctgcgtc
    39421 ataagatccg tttgcaggtc gatggcggcc tgaaaaccgg tgttgatatc atcaaagcgg
    39481 cgattctcgg cgcagaaagc ttcggcttcg gcactggccc aatggtcgcg ctcggctgta
    39541 aatatctgcg tatttgccac ctgaacaact gcgcaacggg tgtggcaact caggatgaca
    39601 aactgcgtaa gaatcactat cacggcctgc cgttcaaggt gacgaattac tttgagttta
    39661 tcgcctgtga aacccgcgag ctgatggcac agctgggggt aacgcggctg gtggatctga
    39721 ttggtcgcac cgacctgctg aaagagctgg acggtttcac cgccaaacag cagaaactgg
    39781 cgctgtcgaa gctgctggag accgccgaac cgcatccagg taaggcactc tactgcaccg
    39841 aaaacaaccc gccgtttgat aacggcctgc tgaacgcgca gttgctgcaa caggcgaaac
    39901 cgtttgtcga tgaacgccag agcaaaacct tctggttcga tatccgcaat accgatcgct
    39961 ccgtgggcgc gtcgctttca ggctatatcg cccagacgca cggcgatcag gggctggcag
    40021 ccgatcctat caaagcgtac ttcaacggca ccgcaggcca gagtttcggt gtatggaacg
    40081 cgggcggcgt ggaactgtac ctgaccgggg atgccaacga ctatgtcggt aaaggcatgg
    40141 cgggcggctt aatcgccatt cgtcctccgg ttggttccgc cttccgcagc catgaagcaa
    40201 gtattatcgg caacacctgc ctgtatggcg cgaccggtgg tcgtctgtat gccgcaggcc
    40261 gcgcgggtga acgtttcggc gtgcggaact ccggtgctat caccgtggta gaaggcattg
    40321 gcgacaacgg ctgtgaatat atgacgggcg gtattgtctg tattctgggt aaaaccggcg
    40381 ttaacttcgg tgcgggcatg accggcggct tcgcttacgt tctcgatgaa agcggtgatt
    40441 tccgcaaacg cgttaacccg gaactggtcg aggtcttaag cgttgacgat ctggcgatcc
    40501 atgaagagca tctgcgcggt cttatcaccg agcatgttca gcatactggc tctcagcgcg
    40561 gtgaagagat tctggcgaac tggtcaacct tcgccactaa atttgcgctg gttaaaccga
    40621 agtccagtga tgtaaaagca ctgctgggtc atcgtagtcg tagcgcagca gagttgcgcg
    40681 tgcaggcgca gtaaggggta gcaacaatga gtcagaatgt ttatcaattt atcgacctgc
    40741 agcgcgttga tccgccaaag aaaccgctga agatccgcaa aattgagttt gttgaaattt
    40801 acgagccgtt ttccgaaggc caggccaaag cgcaggctga ccgctgcctg tcgtgcggca
    40861 acccatactg cgagtggaaa tgtccggtac acaactacat cccgaactgg ctgaagctcg
    40921 ccaacgaggg gcgtattttt gaagcggcgg aactgtcgca ccagaccaac accctgccgg
    40981 aagtttgcgg acgagtctgc ccgcaagacc gtctgtgcga aggttcctgc actctgaacg
    41041 atgagtttgg cgcggtgacc atcggcaaca ttgagcgtta tatcaatgat aaagcgttcg
    41101 aaatgggctg gcgtccggat atgtccggcg tgaagcaaac cggtaaaaaa gtggcgatta
    41161 tcggcgcagg cccggcaggt ctggcgtgtg cggatgtcct gacgcgtaac ggcgtaaaag
    41221 ccgttgtctt cgaccgtcat ccagaaattg gcgggctgct gaccttcggt attccggcct
    41281 tcaagctgga aaaagaggta atgacgcgtc gccgtgaaat cttcaccggc atgggtattg
    41341 aattcaaact caataccgaa gtgggccgcg acgtgcagct ggacgatctg ctgagtgatt
    41401 acgatgccgt cttccttggc gtcgggactt atcagtcaat gcgcggcggg ctggaaaacg
    41461 aagacgccga tggcgtgtac gcagcgctgc cgttccttat cgccaacacc aaacagttaa
    41521 tgggctttgg tgaaacccgc gacgaaccgt tcgtcagcat ggaaggcaaa cgcgtggtgg
    41581 tccttggcgg tggcgacact gcgatggact gcgtgcgtac gtccgtgcgc cagggagcga
    41641 agcacgttac ctgtgcctat cgtcgtgatg aagagaacat gccgggttcc cgccgcgaag
    41701 tgaaaaacgc gcgggaagaa ggcgtagagt tcaaattcaa cgtccagcca ctggggattg
    41761 aagtgaacgg taacggcaaa gtcagcggcg taaaaatggt acgtaccgaa atgggcgaac
    41821 cggatgccaa aggccgtcgc cgcgcggaga tcgttgcagg ttccgaacat atcgttccgg
    41881 cagatgcagt gatcatggcg tttggtttcc gtccacacaa catggaatgg ctggcaaaac
    41941 acagcgtcga gctggattca caaggccgca tcatcgcccc ggaaggcagc gacaacgcct
    42001 tccagaccag caacccgaaa atctttgctg gcggcgatat cgtccgtggt tccgatctgg
    42061 tggtaaccgc tattgccgaa ggtcgtaagg cggcagacgg tattatgaac tggctggaag
    42121 tttaagctct gtcccagcgc cgcctcctca gaaaagagtc ggcgctgaaa ttctcctttc
    42181 tttctggaaa gcgcctcgca taatctgaag aaaaccaatg taatacgctc atctggattg
    42241 ttaccggtag atgcccggcg tatagctgat tccattattc aacgcagcgc catataaagg
    42301 gacagataat ggaatcgctc tcagagagaa cctcaacagg ctaccagcaa atccacgacg
    42361 gtattattca cctggtcgat agcgcccgga cggaaacggt acgtagcgtt aacgcgctga
    42421 tgaccgcgac atactgggaa attggccgac gaattgtcga atttgaacaa ggtggcgagg
    42481 caagggctgc gtatggtgcg cagctaatca agcgactatc aaaggattta agtctaaggt
    42541 ataagcgtgg tttctctgca aaaaacttac gccaaatgag gcttttttac ctcttttttc
    42601 aacatgttga aattcgccag acagtgtctg gcgaattaac accattggga attccgcaga
    42661 caccatctgc agaattccca tcggcaaaga tttggcagac gctgtctgcc aaattgagag
    42721 gctgtttgat atttccacag cttttgtatt gcatctccta ttttcggttt gtatattcat
    42781 gcaaataaac gaaggaaagt tgccgtgatt tacattgaac agcttgaatt aatccataaa
    42841 agtggtgatg tgttgtatcc agtaaaaata accagaaaat catcaggaaa aactgccttc
    42901 caccttgttc cattcggctt aaataagact cacgaccttc tagaggtgga agatgcttca
    42961 gaagcaattc ggctggtgat tgatgaacgt cattccattc gctgcagtac acttacagca
    43021 actattacga ataaaaaggg caagcgaatt aagcggacag gtatatacag tatcaagggt
    43081 gttaacataa aagaatacaa cgtcaggtaa tggcacagag cggtccgtca gttcaggttt
    43141 ggctctgtgc caaagatggg tcaggtcaca cctgatctaa taaatttaag ccattaaatc
    43201 agccttaacc ttcaccacca cctttttaat ctcacccggc tcgccaacaa cgcatcccgg
    43261 cttatgcggc tctcctagca taaacacggc gaacattccc ggtttcagga tgatggcttg
    43321 ctcgttttca atggtgctgc aaagctggta atcatcctca tggtggaact cttcacactg
    43381 acgcgcagtg cctgccatgc caaacagaat ccgttcttca ccgtttaata acagctggat
    43441 atcaatgtat tgctcgtgca gctccgcttt tttctcaacg ggcgattgag tgttaaacgt
    43501 catgacattc ataaaaatat tgtcgccctg taattcgtaa cgacccggcg ctttttcttg
    43561 cggtctggca gctaatgcca gcgttaacgc gtcctgtaac gcaggatgta acccagcaga
    43621 cggtaatgac cgtacttcac ccatcatcat aatttttctc cctgggccaa cagcgctgcc
    43681 ccaagtaaac ctgcatcatg gcggtagtgc gccgccagta aatcaacatg aaataccgct
    43741 ggctcctgcg ccagatacgt ttccaccaac gccagatacc cttctgccag accaacgctg
    43801 ccaccgacca ccacgcactg gcaatcagtt gtggctttaa tatcagcgat cagccttgcc
    43861 agcacatgtg cggagcggtg aatcagttgc tgcgcctgct cgtcgccctg cccggcgcgt
    43921 gtgaaaatag ttctcgcatc cgcgcccatg agttcacctt gcgctgccgc tgcaataccg
    43981 cgaccagaag caatcgcttc cacgcaacct gtgcgtccac agccgcagac tgggccgtgt
    44041 ggatcggcaa gcgtatgccc gatatgcccc gccagaccgc cagggccggt aagcagttta
    44101 ccgccgctca ctacaccgcc gccgacgcca gtggaaacgg tgataaacac catctcggtt
    44161 acatcgcctt ccagcgcctg atactctgcc catgctgcgg cctgcgcgtc gttaatggca
    44221 atggtcggca aattggtaag ttgttccagc gttttgacta acggaaagtg tagcaatcca
    44281 ccgagattat gcggattcag cgccagcaaa ctgccgtcgc gaataattcc ggttgaagcg
    44341 atggcaaccc gctgcgcatg agcttgcaac ggagagacta atgcggataa ggcatcacgc
    44401 aaggcttctg gtgtctggct ggctggcgta ggaagttcac gacgatcgcg gatctgcccg
    44461 tcagcgccaa tcagcgcggc ggcaagttta gtaccgccga tatcaatcgc cagtgtggtc
    44521 atagcaccgc ctttttcatc gctgtgttgt accactgaca aatgtgttca agacgcgtaa
    44581 tggcagaacc gaccgtcacc gcccacgcgc cgtggcgcat cgcatctgcc gcctgagcag
    44641 gcgtgttgta acgcccttcg gcaatcaccc gacatccggc ttcgctcaac gttttcacca
    44701 gcgccagatc cggctcttct ggcgtttcag gcgtggtata gccagaaagc gtagtgccaa
    44761 taatttcggc tcccagcttt tggcatgcca ggccgtcttc cggcgttgag cagtcggtca
    44821 tcgccagtaa accgtgatga tgtatacgtg ccagcagcgt ttcaacaggc accggacgcg
    44881 ggcggtcggt gccgtcaatg gcgataatgt ccgcgcccgc ctgcgccagc gcatcgacat
    44941 cttcaatata ggccgtgatg cgtaccggag aatcctccag atcgcgtttc acaattccaa
    45001 taatcggcac gctcaccacc gcacgcgtgg cttgcagatt tgccacacct tcgatgcgaa
    45061 tggcaaccgc gcccgcctgc tctgccgcta atgccatggc tgcgacgatt tcgggtttat
    45121 cgagcgggct gtcaggaatc ggctggcagg agacaatcag gccaccgtta gcagcgattt
    45181 tttgatccag ttgtgcaagt aacgacatac atcttccctt agcgaaaggc ccggtacata
    45241 gaccgggcaa caggattaac ttttggtttt gactaaatca tttttggcgc tgccaaatgg
    45301 cacggcaccg ctaaatggtt tgccgtcgat agcgtcatga gtacgcaatg cttccgggcg
    45361 cagccaacgc tgaacgcggg aaggcatatc cagaccaatc agcaggatca ccacaaatgt
    45421 caggctgaac gagagcgatg ccagcgcagt acccagatcc agacgttgag cgatcaacgc
    45481 gccgatgatt ggggccagtg cgccacccaa tgcgccaacg ttgtaggtaa agcccaggcc
    45541 cgctgcacgc tggtcggtat cgaaataacc gccaatcagt ttcggtaaga tcccggcgat
    45601 cccttgtcca agcatttgct ggaagaacag taacagaccg agcacccaga cgtttgcgcc
    45661 gccaatcgca aataccggaa taatcagcag ctgcgaggcc agcaggctac aaacgtacgc
    45721 tttgcgggtt cccagccagt caccgaggaa gccacctacg cagcatccca cggccgcgcc
    45781 aaagccacta aagaacagca cattggcgac agtatgcggg ttataagcca gatcggtttt
    45841 cagatacgtt ggcagcagcg cctgaatcgg ccatgagtag aggaaagcaa acaacacgac
    45901 caccatcagc attacgcccg ttggccagcg tttgcctgta ctctgcacca taaagctgat
    45961 aaagattgcg gcgcataaca gcccaagaac agcgacgatc gcggcatttt gcaggttacc
    46021 ggcgaagcag aaccacagcg cagtagccgc cgccagtgtc attacgatat tggcaatgcg
    46081 atgttcacca cggtagagaa tatccaccat tgtgcgtact ggtgctttac ctgcgtgttt
    46141 ctctttccag tcttccgctt ccgggatgtt tttacgcagc cagagagcaa agatgattgg
    46201 caaaatgccg ataaagaaca gcgcacgcca accccagacc ggaaccacca ggctatagac
    46261 ctgagcggca acgacggccc ccacagagaa gcctgaaatc aaaaaaccac tggctttgtt
    46321 acgcagatgt tttggccagc tttcaatgac ataggtggcg ctggaaccgt attcacccgc
    46381 catccccatg ccgatgacca gacgagcgat aaacatggtg atgtagcctg gcgcaaagcc
    46441 gcaggccagc gtcccggccg agaagagaac gatgctggtg accattgcca gacgacgccc
    46501 gtagcggtca cccatagcgc cgagcatcag gccgccgaac cagcgagaga taaaggctgc
    46561 ggagatcaga cttgccgcct gcaccgtcgt cagcccgaat tcaccctgta cttcggtgag
    46621 taccagggcg attaaaacga aatcaaaacc gtcaagcaga tatcccaacc aggcagcgga
    46681 aaatgcgcgc cattgtgcac ggttgagatg gcgataccac gggatattct gggttgtagt
    46741 actcattgtg agtctcccgc ggtgggcgat gcccacacgc tttggtatga aaattgtagg
    46801 gtacagatgc gtttatttcc cctcacccgg taggggcgag cgaggggaaa acaactcacc
    46861 cgcgctcttg catcaactgc tgggccagcg ccttcagttc tggcagatat ttttcatcta
    46921 ccggtccaaa cggtttgcgg cacagcggca cagaaacgac atccatataa tgcaggacag
    46981 ttttcaggcc gcggaatacg ccagttttga tcagtaaatc aatgactttg ttgcactcag
    47041 tttgcagttt ctgcgcggtc tggatatcgc cttctttcag cgccttcacg atcccctgat
    47101 atcgccagcc catgatgttg taagtactgc cgataccacc atcagcgcct gccagcaagc
    47161 cggaagcgaa gatttcgtcg taaccgttat agagcaccag atcaggatgt tcacgacgga
    47221 tctgttccat ctgatagaga tcgccagaag tctgtttcag cgcaccgacg ccaggcaacg
    47281 taaccagggt gttgatctga tccagggtca gttttacccc actcagggct ggaatgttgt
    47341 acacaaccat cggcaaacca tccgctgaat caataattgc ccgatagtga tcgcagtgtt
    47401 cttcaaagct gaaaggatag tagaacggcg tgacggcgga gacggcatcg aagccataac
    47461 gtttagccga tgccgcaagt tgttggcttt cggcggtgct gacgcaaccg acgtgggcga
    47521 tgagtttaat cttacctttc gcctcttcgg cgacgatttc cagtacctgt tcacgctcgg
    47581 aaaggctttg tacaaaggcc tcgccggtcg aaccacccac gtataaaccg tcgatgccct
    47641 gctgaatatt gaactgaacc aggcgacgca gactcgcttt atccagtgct tgttgttggt
    47701 caaaaggagt caggagtgca gccattacgc cacgtaaatt cgttgccata aatacctctg
    47761 aagtgatact tgtctgataa acgatatacc tttatacctg ttataccaga tcaattaagc
    47821 aacaccccat acagaaagct tataatgcga tctgcttcac taaagtggca ttatttcttt
    47881 ttgttggtgg tctgaccgaa agcgtgccag gtagcagaga cgctgttgag atgcgattgc
    47941 aacgcacgat cggcttcgtc aggatcatga cggcggatcg catcaacgat cgcaatatgc
    48001 tgttgatagc taacgttgtt atgctcgtgc agagcctgat cagccacggt tggacgtgcg
    48061 gcgatcagcc agtcgagcag ggcaacgtgg atcgccatga agattgggtt accagggatc
    48121 tccgccagca cgcggtggaa atcaacgtct gaacgaatga atgccgcgtt gttatccagc
    48181 gactgactgt tgatttccag tgcttttgcc agcaaatcga tttgctcatc ggtggcatgt
    48241 tcagccgcat agcgcaccag actggattca aagaacagac gtaattgttc gaaatgggca
    48301 atcccaccgg gatgagaaag gaaatctttc gccatgccgg aaaactcacc gatgatagtg
    48361 tccgcagaag gacgcgagac gcgagcgcgt tcgccgttgt ttatttgcac cagacctttg
    48421 cgttttaacg ctgccagcgc ttcgcgcaca gaaggacgcc cgacgttaaa gaacgccatc
    48481 agttcgcgtt cagacggtaa ttgttcacct tcgccaaatt cacgacggcg gatcatctgt
    48541 tccagctctt cttccaccat ttcggagagt tttttacgcg ccagcgggcg gctacgcaag
    48601 ttgcgaccaa ttgcaggtga agaatcttcg gtttgcgaat caaatgcgtt cataaggccc
    48661 attctgtaag gtcagtgtga ttaacatcat cagtgacatc ctatcacagg attgaaagta
    48721 ggggaaaatg gcaggttttt ctctttgtgc ctcatcatta ccataattaa cggaataatt
    48781 aactattgcg aaaaattaat gtaacgcaga taaacacatc ccgattgaat tattcataag
    48841 actattcaca agcattatga atattatgaa tgtgttctta caaaataatc ataagcgcat
    48901 attttttaat gaaaaatcac atcacctaca attaaaaaca cgacatccgc accataaata
    48961 gacttgcaaa aaatataaca tcattgtttt caatctgccg tttatgggat tgaccgtttt
    49021 cttttgacac ggagttcaac aatgttcggc ataattatat ctgtcatcgt attaattacg
    49081 atgggctatt tgatcctgaa aaactacaaa cctcaggtgg tgctggctgc cgcaggtgtc
    49141 ttcctgatga tgtgcggagt ctggttaggg ttcggtggtg tactcgatcc cgccaaaagc
    49201 agcggctact tgatcgtcga catttataat gaaatcctgc gcatgctgtc caaccgcatt
    49261 gccggattgg ggctgtcgat tatggcggtg ggcggttatg cccgctacat ggagcgcata
    49321 ggagccagtc gcgcgatggt gagcttgcta agccgcccgt taaaactcat tcgctcgccg
    49381 tatattattc tgtcggcaac ttacgtcatc ggccaaatca tggcgcagtt tattaccagc
    49441 gcctccggtc tgggtatgtt gctgatggtc accttatttc cgacgctggt gagtctggga
    49501 gtaagtcgcc tctctgcggt ggcggttatc gcaaccacga tgtccattga gtgggggatt
    49561 ctggaaacga actccatttt tgcagcccag gtcgcgggaa tgaaaattgc cacatacttc
    49621 ttccactacc agcttccggt cgcctcttgc gtcattatct cggtggcgat ctcccacttt
    49681 ttcgtgcaac gcgcttttga caaaaaagat aaaaatatca atcacgaaca ggcagagcaa
    49741 aaaactctcg ataatgtccc gccgctctat tacgccattt tacctgtgat gccgttaatc
    49801 ctgatgctcg gctcgctgtt cctcgcccac gtcgggctga tgaagtcaga actgcatctg
    49861 gtggtggtga tgttactaag tttgacggtg acgatgtttg ttgagttctt ccgcaagcat
    49921 aacttgcgcg aaacaatgga cgatgtgcag gcgttttttg acggcatggg tacgcagttt
    49981 gccaacgtgg taacgctggt ggtcgcgggt gaaatatttg cgaaaggctt aacgacgatt
    50041 ggcactgttg atgcggttat caggggtgcg gagcattctg gtctgggcgg tattggcgtg
    50101 atgattatta tggcgctagt cattgccatt tgtgccattg tgatgggctc tggcaatgcg
    50161 ccgtttatgt catttgccag tcttattccg aatatcgcag ccggactaca tgtaccagcg
    50221 gttgtaatga ttatgccgat gcattttgcc acgacgctag cgcgcgcggt ttcgccgatt
    50281 actgcggtgg tggtcgttac gtcaggaatt gcaggcgttt cgccttttgc ggtggtgaag
    50341 cggacagcga tccccatggc agtcggtttc gtggtgaata tgattgccac aatcacgcta
    50401 ttttattaag tcattaaaaa gacaaaacag gccgcctggg cctgttttgt attacttcac
    50461 aacacgtaat gccggtcgac caccgcgtgg tggctgcgga ggttcatcgt caggatgagt
    50521 gtcatcatcg tgatctggct tgtcgccatc aataaccgac ataacggttt cgttgtctgc
    50581 cgatgcctct tcatcattca tgatgctggt atcttcatcg taggcagctt caggctcaaa
    50641 catcgtgcct gcgccatttt cacgggcgta gatagccagc acggcagcca gcggcacaga
    50701 aacctgacgc ggaatgccac caaagcgcgc gttaaagcgc acctcatcat tcgccagttc
    50761 cagattgccg acagcacgcg gcgcaatgtt gagtacgatt tgcccgtcac gcgcatattc
    50821 cataggaacc tgcacgccag ggagcgtcac atccaccacc aggtgcggcg tgagctggtt
    50881 atccagcaac cactcataga atgcacgcag cagataggga cgacgtggtg ttagctgtga
    50941 caaatccata cagattaact ccggcccaga cgcatttcac gttctgcttc agttaaagaa
    51001 gcaaggaaag agtcacgctc aaagacgcgg gtcatatagc ctttcagctc tttcgcaccc
    51061 gggccgctga gctcgatacc cagttgcggc agacgccaca gcagcggagc aagatagcaa
    51121 tcgaccaggc tgaactcatc gctcaggaag tacggcttct gaccgaagac cggcgcaatc
    51181 gccagcagtt cttcgcgcag ttgcttacgt gcggcatctg cttcagaagc tgaaccgttg
    51241 atgatggtgt tcatcagcgt gtaccagtct ttttcgatgc gatgcatgta cagacggctt
    51301 tcaccgcgag ctaccgggta aacaggcatc agtggcggat gcgggaaacg ctcatccaga
    51361 tattccataa tgatgcgaga ttcccacagg gtcagctcac gatccaccag ggtcggaacg
    51421 ctctgattcg ggttgaggtc aatcagatcc tgaggcggat tgtccttttc cacgtgttcg
    51481 atctcgaaac ttacaccttt ctcagccagc acaatgcgga cctgatggct atagatgtca
    51541 gtaggaccgg aaaacagcgt cattaccgaa cgtttgttgg cagcgacagc catgaaaacc
    51601 tccaggtata gtcagaattt ttactgctac cagccaccag gtggccagtc agaagttgtg
    51661 ttacccaata aggaacgact ctctttgttc gaaaatcaaa caaaaaatga gcaatacccg
    51721 acatttgggc agaaaattgg atgatagttt accagatttt gcgaccattg tggtgagtgg
    51781 atgccggaaa tggggaaaaa gagatgcgct ttagtctgaa atagttgact tagaccctta
    51841 ttggcgatgt ggtttttgtt ttgcctgtct ttcagacggc agcaaaaagc aacttcccat
    51901 tttttacgct gattcagaat ttagccataa aaaaacccgc cgaagcgggt tttttcgtaa
    51961 attgttttct gccggagcag aagccaatta acgtttggag aactgcggac gacgacgtgc
    52021 tttacgcaga ccgactttct tacgttcaac ctgacgagcg tcacgagtaa cgaagccagc
    52081 tttacgcagt tcagaacgca gggactcgtc gtattccatc agagcgcggg tgataccgtg
    52141 acggatcgca ccagcctgac cagagatacc accaccttta acggtgatgt acaggtccag
    52201 tttctcaacc atgtcgacca gttccagcgg ctgacgaact accatgcggg cagtttcacg
    52261 accgaagtac tgttccagag aacgttggtt gattacgatt ttaccgttgc ccggtttgat
    52321 gaaaacgcga gctgcggaat ttttgcggcg accagtgccg tagtattgat tttcagccat
    52381 tgcctataat cccgattaga tgtcaagaac ttgcggttgc tgtgccgcgt ggttgtgctc
    52441 gttacccgcg taaactttca gtttacggaa catagcacga cccagcgggc cttttggcaa
    52501 catgccttta accgcgattt caatcacacg ctcaggacgg cgagcaatca tctcttcaaa
    52561 gctcacttgt ttgataccac cgatgtggcc ggtgtggtga tagtacactt tgtcagtacg
    52621 cttgttgccg gttacagcaa ctttgtcagc gttcagaacg atgatgtaat caccggtatc
    52681 tacgtgcgga gtgtattccg ctttgtgctt accgcgtagg cgacgagcca gttcagtagc
    52741 cagacggccc agagttttac cggtcgcgtc aacaacatac cagtcgcgtt ttacggtttc
    52801 tggtttagct gtaaaagttt tcattaaaag cttacccaat aaatagttac acgttggtga
    52861 acacccaaac gtcttcaatt gttgaggttc acacgacaaa gtccggcaaa cctacccctt
    52921 cgaatagcct atgccagcac acaaaaagtt ttgggaaaaa aactttcttg taacgtgggg
    52981 tcgcaggatt atagagaagt cggggtcaaa gatcgacccc tttttgtgat ttgtgacagg
    53041 ttttaacccg ccaaatgctc gcgcttcaga tactcttcgc tttgcatctc ttgcagacgt
    53101 gacaggcaac gctggaactc aaacttcagc cgatcgccct gataaatttc atacagcggc
    53161 acttctgcac tcaccactaa tttgacatgg cgctcgtaaa actcatccac cagcgcaata
    53221 aagcgccgcg cttcgctctc catcaaccgc gtcataactg gtacatcaaa caacatgacc
    53281 gtatgaaaga gacgtgagag cgcaatatag tcatgctgac tgcgggcgtc gacgcacagc
    53341 gtagtaaaag agaccgccag cgtctggttc tcgacgccca ttgtcgctaa tggccgatgg
    53401 ttgatttcta acgtcggtga attttctcgt ttcccccccg ccagcgccaa ccatagttta
    53461 tccatttgcg cccgggtttc atcgtggagt ggcgaaagcc acagatgcgc ctgagtgagt
    53521 gtacgcagtc gataatcaac accagcgtcc acgttcatta catcacaatg ctgtttaatg
    53581 gcatcgattg caggcagaaa acgcgcacgt tgcaagccat ttcgataaag ttcgtccggc
    53641 ggaatatttg acgtcgctac cagggtaata ccgcgggcga acaaggcttt catcagaccg
    53701 ccaagtagca tggcatcggt aatatcagaa acaaaaaatt cgtcaaaaca gagcacgtca
    53761 gtttcggctt taaagcgatc ggcaataatt tccagcggat cggtctgccc ctgtaaggca
    53821 gttagctctt catgcacccg cagcataaaa cggtgaaagt gcaggcgctg tttccgctct
    53881 cccggcaggc tttgatagaa aaggtccatc agccaggttt tcccgcgtcc tacaccgccc
    53941 cacatatata agccacgcac tggcgtatgc tttgtgtctt cgcgtttacc ccacagctta
    54001 ccgacccgcg ccattagccc actcgtcctg ggggctggtg gcgtgctatt gatgagttcc
    54061 tgataaataa tttccaggcg gctgacggcc tctttttgaa cgtcgtcggg ttgatggctg
    54121 ccttcattga gcgccttcag gtattgcgat gttggggtaa cgctttgcat gatcttattg
    54181 ttattccttg aataatcggt gcgccgttgt tcacggttga cgaaaaaaag gccgttctac
    54241 actacgcgat atgcagtcgg gattccactt ctgtggaatt aacggttata gtggcataat
    54301 cagccgcagg catggagcct gaagccaaca ccctacggaa acaaaagaca acgggagatg
    54361 ttcatgacct gggaatatgc gctaattggg ttagtcgtcg gcatcattat tggtgctgtg
    54421 gccatgcgtt ttggtaatcg taaactacgc cagcaacagg cgttgcagta cgaactggaa
    54481 aagaataaag ctgaactgga cgagtatcgc gaagagctgg ttagccactt tgcccgcagc
    54541 gcggaattac tggataccat ggcgcacgac tatcgccagc tgtatcagca catggcgaaa
    54601 agctccagca gcctgctgcc ggaactgtct gctgaagcaa acccgttccg taatcgtctg
    54661 gcagagtctg aagccagcaa cgatcaggca ccggtgcaga tgcctcgcga ctattctgaa
    54721 ggcgcatccg gcctgctgcg tactggcgcg aagcgcgact aatttatttt tcgggcgcag
    54781 ccattgcgcc ctcctcttct ctccctcccc gactatcatt taatctggtg tctcattgtt
    54841 agccgtctga aaattcaata acatcaaact gttttgaatc tcttttctta tcattcaggt
    54901 acgagagcag gaataatgaa aaaacaaccc cagctgttga gtgcattagc gttaagtgtc
    54961 gggttaactc tctcggcgtc atttcaggcc gtcgcgtcga ttccaggcca ggttgccgat
    55021 caggcccctc tccccagtct ggctccaatg ctggaaaaag tgcttccggc agtggtgagc
    55081 gtacgggtgg aaggaacggc cagtcaggga cagaaaatcc cggaagaatt caaaaagttt
    55141 tttggtgatg atttaccgga gcaacctgca caacccttcg aaggtttagg ctccggtgtc
    55201 atcatcaacg ccagtaaagg ctatgtgctg accaacaacc atgtgattaa tcaagcacag
    55261 aaaatcagta ttcagctcaa tgatgggcgc gagtttgatg caaaactgat tggtagcgat
    55321 gaccagagcg atatcgccct gttacaaatt caaaacccga gcaaattaac gcaaatcgct
    55381 attgccgact ccgataaatt gcgcgtcggt gattttgccg tagcggtcgg taacccattt
    55441 ggccttgggc aaaccgccac ctctggcatt gtttccgcat taggacgcag cgggttgaat
    55501 cttgaaggtc tggaaaactt tatccagaca gatgcttcca ttaaccgcgg taactccggc
    55561 ggtgcactgt taaaccttaa cggtgagtta attggcatca acactgcaat ccttgcgcct
    55621 ggcggcggga gcgtcgggat tggatttgcc atccccagta atatggcgcg aacactggcg
    55681 cagcagctta tcgactttgg tgaaatcaaa cgcggtttgt taggcatcaa aggcaccgag
    55741 atgagtgccg atatcgccaa agccttcaac cttgacgtgc agcgtggcgc gtttgtcagc
    55801 gaagtgttgc caggttctgg ctcggcgaaa gcgggcgtca aagcgggcga tattattacc
    55861 agcctcaacg gcaaaccgct gaatagcttt gctgagttgc gctctcgtat cgcgaccacc
    55921 gagccgggca caaaagtgaa acttggcctg ctgcgtaacg gcaaaccact ggaagtagaa
    55981 gtgacgctcg ataccagcac ctcttcgtcg gccagcgctg aaatgatcgc gccagcgctg
    56041 gaaggtgcaa cgttgagcga tggtcagcta aaagatggcg gcaaaggtat taagatcgat
    56101 gaagttgtca aaggaagccc agctgctcag gctggcttgc aaaaagacga tgtgatcatt
    56161 ggcgtcaacc gcgatcgggt gaactcgatt gctgaaatgc gtaaagtgct ggcggcaaaa
    56221 tcggccatca tcgccctgca aattgtacgc ggcaatgaaa gcatctatct gctgatgcgt
    56281 taatgtcgta aaccgggcat caggcttacg tgtgatgtcc ggttaactcg tggtatgctg
    56341 ctgccgttcc cttttttaat gacgcctcca tcatgtttgt gaagctctta cgttccgttg
    56401 cgattggatt aattgtcggc gctattctgc tggttgccat gccttcgctg cgcagcctta
    56461 acccgctttc cactccgcaa tttgacagta ccgatgagac gcctgccagc tataatctgg
    56521 cggttcgccg cgccgcgcca gcggtggtta acgtttacaa ccgtggtttg aacaccaact
    56581 ctcacaacca gcttgagatc cgcaccctgg gatccggtgt aatcatggat caacgcggtt
    56641 atatcatcac caataaacac gtcatcaacg acgccgatca gatcatcgtc gccttacagg
    56701 atggtcgtgt atttgaagca ttgctggtgg gatctgactc tctaaccgat ctggcggtac
    56761 ttaaaattaa tgccactggc ggtttaccta ccattccaat caatgcacgt cgcgtaccgc
    56821 atattggcga cgtggtactg gcgatcggta acccgtacaa cctcgggcag accattaccc
    56881 aggggattat tagtgccacg ggtcgaattg gtctgaaccc gaccgggcgg caaaacttcc
    56941 tgcaaaccga tgcttccatt aaccacggta actctggcgg cgcgctggtg aactcgctgg
    57001 gcgaactgat gggcattaac acgctgtcgt ttgataagag taacgatggc gaaacgccgg
    57061 aaggtatcgg ctttgcgatt cctttccagt tagcaaccaa aattatggat aagctgatcc
    57121 gcgatggtcg cgtgatccgc ggctacattg gcattggcgg acgtgagatc gcaccgctgc
    57181 acgcacaggg cggtggtata gatcaactgc aagggatcgt ggttaatgaa gtgtcacctg
    57241 acggcccggc ggcgaatgcg ggtattcagg tcaacgatct gattatttcg gtggataaca
    57301 aaccggccat ctctgctctg gagacgatgg atcaggtggc ggaaattcgc cctggttcgg
    57361 tgatcccggt tgtagtgatg cgtgatgata agcagttaac gctgcaggtc accattcagg
    57421 aatatccggc aaccaattaa gtcgagtgct caaaacaaaa aaccggagtc tgtg
//
LOCUS       DAKISA010000013        55869 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.013, whole genome shotgun sequence.
ACCESSION   DAKISA010000013 DAKISA010000000
VERSION     DAKISA010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55869
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.013"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            90..587
                     /gene="gstA"
                     /locus_tag="OVW45_000849"
                     /pseudo
     CDS             90..587
                     /gene="gstA"
                     /locus_tag="OVW45_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
     gene            complement(631..1494)
                     /gene="pdxY"
                     /locus_tag="OVW45_000850"
     CDS             complement(631..1494)
                     /gene="pdxY"
                     /locus_tag="OVW45_000850"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCV5525367.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEVLEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(1553..2827)
                     /gene="tyrS"
                     /locus_tag="OVW45_000851"
     CDS             complement(1553..2827)
                     /gene="tyrS"
                     /locus_tag="OVW45_000851"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCV5525368.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            complement(2956..3612)
                     /gene="pdxH"
                     /locus_tag="OVW45_000852"
     CDS             complement(2956..3612)
                     /gene="pdxH"
                     /locus_tag="OVW45_000852"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCV5525369.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            complement(3671..4000)
                     /gene="mliC"
                     /locus_tag="OVW45_000853"
     CDS             complement(3671..4000)
                     /gene="mliC"
                     /locus_tag="OVW45_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCV5525370.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            complement(4098..5207)
                     /gene="anmK"
                     /locus_tag="OVW45_000854"
     CDS             complement(4098..5207)
                     /gene="anmK"
                     /locus_tag="OVW45_000854"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCV5525371.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            5481..5948
                     /gene="slyB"
                     /locus_tag="OVW45_000855"
     CDS             5481..5948
                     /gene="slyB"
                     /locus_tag="OVW45_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCV5525372.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(5995..6429)
                     /gene="slyA"
                     /locus_tag="OVW45_000856"
     CDS             complement(5995..6429)
                     /gene="slyA"
                     /locus_tag="OVW45_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCV5525373.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            6630..6866
                     /locus_tag="OVW45_000857"
     CDS             6630..6866
                     /locus_tag="OVW45_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCV5525374.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            6869..7726
                     /locus_tag="OVW45_000858"
     CDS             6869..7726
                     /locus_tag="OVW45_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCV5525375.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     RVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            7747..9738
                     /locus_tag="OVW45_000859"
     CDS             7747..9738
                     /locus_tag="OVW45_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCV5525376.1"
                     /translation="MRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLDEPYWAMT
                     SAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSAWLGFCTW
                     ACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGILCGGMMM
                     MILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQILTMNLLRI
                     QAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREILEQLLTAL
                     ASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRLQSGVDDH
                     ARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLAAISCVLY
                     SAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLATMQLLKLQ
                     MPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNENLAKIVGVALAWLAFAILRPGSD
                     ARKSRRHIRALRRDFVDQLSRHPTLSGSEFESLTYHHVSQLSNSQDALARRWLLRWGV
                     VLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATLEELQRIC
                     DSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(9739..10260)
                     /gene="sodC"
                     /locus_tag="OVW45_000860"
     CDS             complement(9739..10260)
                     /gene="sodC"
                     /locus_tag="OVW45_000860"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCV5525377.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(10341..11237)
                     /locus_tag="OVW45_000861"
     CDS             complement(10341..11237)
                     /locus_tag="OVW45_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCV5525378.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLKSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(11286..11525)
                     /locus_tag="OVW45_000862"
     CDS             complement(11286..11525)
                     /locus_tag="OVW45_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCV5525379.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            11628..12227
                     /gene="nemR"
                     /locus_tag="OVW45_000863"
     CDS             11628..12227
                     /gene="nemR"
                     /locus_tag="OVW45_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCV5525380.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKIAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGHENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            12264..13361
                     /gene="nemA"
                     /locus_tag="OVW45_000864"
     CDS             12264..13361
                     /gene="nemA"
                     /locus_tag="OVW45_000864"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCV5525381.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRRAELNPQRAESFYGGGAEGYTDYPTL"
     gene            13442..13849
                     /gene="gloA"
                     /locus_tag="OVW45_000865"
     CDS             13442..13849
                     /gene="gloA"
                     /locus_tag="OVW45_000865"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCV5525382.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            13952..14599
                     /gene="rnt"
                     /locus_tag="OVW45_000866"
     CDS             13952..14599
                     /gene="rnt"
                     /locus_tag="OVW45_000866"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCV5525383.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            14692..19307
                     /locus_tag="OVW45_000867"
                     /pseudo
     CDS             14692..19307
                     /locus_tag="OVW45_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(19358..19705)
                     /gene="grxD"
                     /locus_tag="OVW45_000868"
     CDS             complement(19358..19705)
                     /gene="grxD"
                     /locus_tag="OVW45_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCV5525384.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGKLVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            20039..20854
                     /gene="mepH"
                     /locus_tag="OVW45_000869"
     CDS             20039..20854
                     /gene="mepH"
                     /locus_tag="OVW45_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCV5525385.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKPQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20982..21563
                     /gene="sodB"
                     /locus_tag="OVW45_000870"
     CDS             20982..21563
                     /gene="sodB"
                     /locus_tag="OVW45_000870"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCV5525386.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(21709..22878)
                     /locus_tag="OVW45_000871"
     CDS             complement(21709..22878)
                     /locus_tag="OVW45_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCV5525387.1"
                     /translation="MKIDYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(23044..23133)
                     /gene="ynhF"
                     /locus_tag="OVW45_000872"
     CDS             complement(23044..23133)
                     /gene="ynhF"
                     /locus_tag="OVW45_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCV5525388.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            23432..24457
                     /gene="purR"
                     /locus_tag="OVW45_000873"
     CDS             23432..24457
                     /gene="purR"
                     /locus_tag="OVW45_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCV5525389.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLVGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(24454..25386)
                     /locus_tag="OVW45_000874"
     CDS             complement(24454..25386)
                     /locus_tag="OVW45_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCV5525390.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            25499..26710
                     /locus_tag="OVW45_000875"
     CDS             25499..26710
                     /locus_tag="OVW45_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCV5525391.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGMTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIIPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEAGCQNH
                     GNAEVAHSESH"
     gene            27001..28149
                     /gene="cfa"
                     /locus_tag="OVW45_000876"
     CDS             27001..28149
                     /gene="cfa"
                     /locus_tag="OVW45_000876"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCV5525392.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(28189..28830)
                     /gene="ribE"
                     /locus_tag="OVW45_000877"
     CDS             complement(28189..28830)
                     /gene="ribE"
                     /locus_tag="OVW45_000877"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCV5525393.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            29045..30418
                     /gene="mdtK"
                     /locus_tag="OVW45_000878"
     CDS             29045..30418
                     /gene="mdtK"
                     /locus_tag="OVW45_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCV5525394.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(30459..31715)
                     /locus_tag="OVW45_000879"
     CDS             complement(30459..31715)
                     /locus_tag="OVW45_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCV5525395.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            32023..32099
                     /locus_tag="OVW45_000880"
     tRNA            32023..32099
                     /locus_tag="OVW45_000880"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32057..32059,aa:Val,seq:gac)
     gene            32104..32180
                     /locus_tag="OVW45_000881"
     tRNA            32104..32180
                     /locus_tag="OVW45_000881"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32138..32140,aa:Val,seq:gac)
     gene            32288..32593
                     /locus_tag="OVW45_000882"
     CDS             32288..32593
                     /locus_tag="OVW45_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCV5525396.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            32719..34323
                     /locus_tag="OVW45_000883"
     CDS             32719..34323
                     /locus_tag="OVW45_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HCV5525397.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(34335..35147)
                     /locus_tag="OVW45_000884"
     CDS             complement(34335..35147)
                     /locus_tag="OVW45_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525398.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIVTAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(35151..35936)
                     /locus_tag="OVW45_000885"
     CDS             complement(35151..35936)
                     /locus_tag="OVW45_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCV5525399.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKEVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMCGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(35933..36601)
                     /locus_tag="OVW45_000886"
     CDS             complement(35933..36601)
                     /locus_tag="OVW45_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCV5525400.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLLGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(36665..37303)
                     /locus_tag="OVW45_000887"
     CDS             complement(36665..37303)
                     /locus_tag="OVW45_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCV5525401.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(37316..39418)
                     /locus_tag="OVW45_000888"
     CDS             complement(37316..39418)
                     /locus_tag="OVW45_000888"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HCV5525402.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            complement(39439..40065)
                     /locus_tag="OVW45_000889"
     CDS             complement(39439..40065)
                     /locus_tag="OVW45_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCV5525403.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(40521..40730)
                     /gene="fumD"
                     /locus_tag="OVW45_000890"
     CDS             complement(40521..40730)
                     /gene="fumD"
                     /locus_tag="OVW45_000890"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCV5525404.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            41287..42699
                     /gene="pykF"
                     /locus_tag="OVW45_000891"
     CDS             41287..42699
                     /gene="pykF"
                     /locus_tag="OVW45_000891"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCV5525405.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            43009..43245
                     /gene="lpp"
                     /locus_tag="OVW45_000892"
     CDS             43009..43245
                     /gene="lpp"
                     /locus_tag="OVW45_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCV5525406.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(43308..44312)
                     /gene="ldtE"
                     /locus_tag="OVW45_000893"
     CDS             complement(43308..44312)
                     /gene="ldtE"
                     /locus_tag="OVW45_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCV5525407.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIVPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(44461..44877)
                     /gene="sufE"
                     /locus_tag="OVW45_000894"
     CDS             complement(44461..44877)
                     /gene="sufE"
                     /locus_tag="OVW45_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCV5525408.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLTELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAHGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVSFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            complement(44890..46110)
                     /gene="sufS"
                     /locus_tag="OVW45_000895"
     CDS             complement(44890..46110)
                     /gene="sufS"
                     /locus_tag="OVW45_000895"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCV5525409.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEIITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            complement(46107..47378)
                     /gene="sufD"
                     /locus_tag="OVW45_000896"
     CDS             complement(46107..47378)
                     /gene="sufD"
                     /locus_tag="OVW45_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCV5525410.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKMAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            complement(47353..48099)
                     /gene="sufC"
                     /locus_tag="OVW45_000897"
     CDS             complement(47353..48099)
                     /gene="sufC"
                     /locus_tag="OVW45_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCV5525411.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            complement(48109..49596)
                     /gene="sufB"
                     /locus_tag="OVW45_000898"
     CDS             complement(48109..49596)
                     /gene="sufB"
                     /locus_tag="OVW45_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCV5525412.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            complement(49605..49973)
                     /gene="sufA"
                     /locus_tag="OVW45_000899"
     CDS             complement(49605..49973)
                     /gene="sufA"
                     /locus_tag="OVW45_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCV5525413.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            complement(50521..50709)
                     /locus_tag="OVW45_000900"
     CDS             complement(50521..50709)
                     /locus_tag="OVW45_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCV5525414.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSTELKEEIYFAWRLGVH"
     gene            complement(50809..51219)
                     /gene="menI"
                     /locus_tag="OVW45_000901"
     CDS             complement(50809..51219)
                     /gene="menI"
                     /locus_tag="OVW45_000901"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCV5525415.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            complement(51216..54272)
                     /locus_tag="OVW45_000902"
     CDS             complement(51216..54272)
                     /locus_tag="OVW45_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCV5525416.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKTNTTIGRIYNTVY
                     QRCRLQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            54661..55773
                     /gene="ydiK"
                     /locus_tag="OVW45_000903"
     CDS             54661..55773
                     /gene="ydiK"
                     /locus_tag="OVW45_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCV5525417.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGSIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
BASE COUNT        13449 a        14693 c        13686 g        14041 t
ORIGIN      
        1 ggccttcccg agagtggtaa aggagaggcc ctggaaatac cccacagccc cgaaaaagag
       61 ccagtcagtt gcttaactga ctggcattac tcgaaaacgg tgacgattac tttgccgtta
      121 accctaaggg gcaggtgcca gcattgctgc tggatgacgg tactttgctg acggaaggcg
      181 tagcgattat gcagtatctt gccgacagcg tccccgaccg ccagttgctg gcaccggtaa
      241 acagtatttc ccgctataaa accatcgaat ggctgaatta catcgccacc gagctgcata
      301 aaggtttcac acctctgttt cgcccagata caccggaaga gtacaaaccg acagttcgcg
      361 cgcagctgga taagaagctg caatatgtga acgaggcact gaaggatgag cactggatct
      421 gcgggcaaag atttacaatt gctgatgcct atctgtttac ggttctgcgc tgggcatacg
      481 cggtgaaact gaatctggaa gggttagagc acattgcggc atttatgcaa cgtatggctg
      541 aacgtccgga agtacaagac gcgctgtcag cggaaggctt aaagtaatag ccatcggccc
      601 acaggtcgtt gtgggccgaa atgtgatatt tcagagcttt gttgcgctga agtaatgttc
      661 tggtttggca atacgatcct gagcagccac cacttgcagc tcatattcct gcattgcttt
      721 ggtggtcacc atgatttcgt agactgcagc ggtcacatgt tccaggacct cctgcagcgt
      781 tgccccctga agcagtttca ccagcagtaa accgctcgtc acatcaccaa cacctaccgg
      841 ctggcgcata ccaaaatcca ccagtggacg gctgatatgc caggcttcat cggcggtgac
      901 cagcagcatt tcaaaacggt cacggctgta gccagctcgc gccaggtgtt taaccaacac
      961 aatttgtggc ccttgcgcaa tgagttcgcg cgctgccaga accgcttctt cgacgttatt
     1021 taccgcatgc tcacagagta tttccagctc aaccagattc ggcgcaatga tatcgctggc
     1081 aggcaaaccg tgccgcacat gaaactctgc gacacccggt gcaacgatac agcctttttc
     1141 cggatgcccc attaccggat cgcaaaaata tttcgcctgc ggattcgctg ctttcacctg
     1201 acggacgata ccgaggatat gttcaccctg ctccgccgat cccagatagc cacttaacac
     1261 ggcatcacag gtgtgtaatt tatcaatagc ggcaatgcct tgcacaattt cggttaaatg
     1321 gctgggcggc atcacgcagc cagtccattt gccgtattgg gtgtgattag aaaattgaac
     1381 ggtgttcagc ggccagacgt tcgcgcccag gcggcgcatc ggaaactctg ccgcactgtt
     1441 acccgcatga ccataaacaa cgtgagactg gatagcgaga atatttttca tcatgttccc
     1501 tgtataaaaa ccagggggag tgatttctca ctcccccttt ccacttaagg cattatttcc
     1561 agcaaatcag acagtaattc tttttgccgc gacgcagtaa ggtaaaacga ccaaacagac
     1621 gatcttcttc tttaaagaag tattcaggat cggactgttt ttcaccgtta atggtgatgg
     1681 cattggaggc gatagtttta cgcgcctgac cacgggaagg ttgcagttca gaatcgacca
     1741 gcgcctgcat caggtctgcg cccttttcca tctcaaccat cggtacgccg tcctgcgcca
     1801 gctgttcgaa gtccgcttca ctcagcgcac tcaaggaacc gctgaacagg cattcggtaa
     1861 tacgttttgc cgcctgcaaa ccttcttcac cgtgaaccag acgagtcacc tgctccgcca
     1921 gtacatactg ggcgcgcggt gctttaccgc tgtttttatc ttcttcttcc agggcgttga
     1981 tctcttcaat gctcataaag gtgaagaact tcaggaagcg gtaaacgtcg gcatccgcag
     2041 tgttgatcca gaactggtag aatttgtacg ggctggtttt cttcggatcc aaccagactg
     2101 cgccgccttc agttttacca aatttggtgc cgtctgcttt ggtgatcagc ggaacggtca
     2161 ggccaaacac ctgattctga tgcagacgac gggtcaggtc gataccagaa gtgatgttac
     2221 cccactggtc agaaccacca atttgcagca ccacaccgta ctgtttgttc agacaggcga
     2281 agtcataacc ctgcaacagg ttgtaggaaa actcagtgaa cgaaatcccc tgatcttcac
     2341 ggttgagacg ctgcttaacc gcttctttgt tgatcatctg gttaacggag aagtgtttgc
     2401 caatatcgcg caggaaggtc agcacattca tattgccgaa ccagtcatag ttgttcgccg
     2461 cgatagcaga gttttctcca cagtcgaaat cgaggaacgg ggcaacctgc ttgcggattt
     2521 tgtccaccca ctcctgaaca gtttcttcgg tgttcagctt acgctcggca gctttgaagc
     2581 tcgggtcgcc aatcagaccc gtcgcgccgc ctaccagcgc aaccggcttg tggcccgcct
     2641 gctggaagcg tttcaggcat aacaatggaa caagatgccc caaatgcaag ctgtcagcgg
     2701 taggatcgaa gccgcaatag agcgcgatcg ggccttgcgc cagtcgctct gctaacgctt
     2761 cctcgtccgt cacctgggct accagccccc gctcttgcaa ttgtttaatc aagttactgc
     2821 ttgccatcaa aatctccatg tatataacga ctgcaccttt gccggtacac gacttttcgc
     2881 cagatgcgaa agagacatag aataaagtgc cagaatcagg agtaccagcg attaaagcaa
     2941 gatttttgca tcttttcagg gtgcaagacg atcaatcttc cacgcatcat tttcacgctg
     3001 gtacaaaaag cggtcatgca ggcgatgctc accgccctgc cagaactcaa tctgttcaag
     3061 gctgacgcga aaaccgcccc aaaagctcgg caatggcact tcgccctgtt gaaacttctg
     3121 cttcagctcc aggaatttac tttcaaggat accgcgggca gaaatgcgac tggactgctt
     3181 cgaaacccat gcaccaatct ggctatcacg cgggcggcta tgaaaatatt tcatcacttc
     3241 gagagtcgaa agtcgttccg ctttaccgat caccatcacc tggcgctcaa gggtatgcca
     3301 cgggaacagc aggctaacgc gcggattatt ttcaatttga tgtgctttac ggctgccgag
     3361 gttggtgtaa aacaccatgc ctttttcgtc gtaatgtttg agtaaaacga tgcgttgata
     3421 aggctgagca tgttcatcca cggtagcgac caccatcgcg gtagggtctg ccagtttggc
     3481 ttcacacgcc tgagagagcc aacgttcaaa aagggttaat ggatcggcgg gaagatcgcg
     3541 acggcgtaac ccgcctttgg tgtattcacg gcgcagatgc gcgatttgct gcaattcgtc
     3601 gttatcagac atggttttct ttacggattg tcagtgggtg acgctattgt gcgccgcccc
     3661 tggaaaaatc tcaacgctgt ggattttgta actgacagtt attcaatacg atgcggtcgc
     3721 gtttatagac agtcgcttca tcgcctttcg accagaaaac atagattccg tcagtgtaac
     3781 gtgcaccaga cgctgaaatg ccctgtttga gatgcagtag ttgattatcg taaacaaaac
     3841 tgacttcctg gcgcggatta ttcagtttga ccgtcaacgg tttttcatca cactggtatt
     3901 ccagcgtagc ggtttgcatg tgctcaacca gttgattgaa cgcgctacag ccggtgagaa
     3961 gcaccggcaa acagatcagt aacagttttt tcatagacat ttcccgaaga ctttcctggt
     4021 ctggagggca atacgccctc cctaacgttt caagtgtaac ggcagacgcg gtaagaaaaa
     4081 ttcagttaac tctgatatca cgggttagca gggaaaatag cccccagtac cgtctcctgg
     4141 cttgcgccag tgacggaagg cagatttcct ggtaatcccg ccagcgtccg ccaggcaagc
     4201 caggcgaatg ccaatgcttc catgtcatcg ccactaatgc caacggcatc ggtggtggtg
     4261 acttctgtgc ctggcagtaa tgccgccaga cgcgccatga gtagcgggtt acgactacct
     4321 ccaccacata ccatcaatcg ttcgcagcca ccgctcaaca aaacttgttc agaaatggtc
     4381 acggcggtga gttctgccag tgtggcctga acatcacggg gatcaacacc cggaaaatgg
     4441 cgtaaatggc gctccagcca gccgtagtta aagtattcgc gtccggtgct tttcggtgca
     4501 ggttgcgaga aatatgggtc gctgagcata ttttgcagca gtgggagaat aactttaccc
     4561 gcccgtgccc actcggcatc tttatcgtaa ggtttaccgg cctgacgcca gatccaggca
     4621 tccatcagca tgttaccagg accggtatcg tagccgccaa ccggctgccc aggaatgagc
     4681 agtgacagat tggcgatgcc gccaatattg agcaccattc gtcgctcggt tgggtgagcc
     4741 agcaaggcat gatggaacgc aggtaccagc ggtgcgcctt gtcctcccaa ggcaatatcg
     4801 cgacggcgaa aatcaccgac aaccgtaatt ccggtgcgtg ccacaatttg attgttatcg
     4861 ccaatctgta aagtgtgtgg tgccacgccc gtcggttcat gccagacggt ttgaccgtga
     4921 cagccgatcg caactatatc tctcgcctgc aggttttgtt ccttaagcaa ggcattgacc
     4981 gcatcagcaa aaagtcgccc gagttgagta tcaagctgtc caaactgcga aagtgtaagc
     5041 tgctggccct ggcaaatatc cagtacagcc tgtttcagag acaccgggat cggccagctc
     5101 aagcttgcca gctgtgcgac ccggtgttca tctattgtcg ccaacacaac atcaacacca
     5161 tcaaggctgg tgcctgacat aacgccaata aagcggcccg atttcatagt tcatcctttt
     5221 tcaatctgac gtttgcgcac cactcaaaca taaacttttc gtggatacca tgcggaatga
     5281 ccgattttta ccgttggtag taaaacatta tcttcaaatc aataatcatc atgaatgttt
     5341 tgtttataat tggttgatcc tactttcatt atgatttgct catatttggt agaacatgta
     5401 accatggatt cacatatgcc atatactttg accatgaggg atgcttgcgt ggcgtttcat
     5461 ggtgaacagg agatttttca atgattaaac gcgtattggt tgtttcaatg gtaggtctgt
     5521 ctcttgtcgg ttgtgttaat aacgacaccc tgtcagggga tgtttatacc gcttctgaag
     5581 cgaaacaagt acagaatgtc agctatggca ccatcgttaa cgtacgtccg gtacagattc
     5641 agggcggtga tgattccaac gttatcggtg caattggcgg tgctgttctt ggtggtttcc
     5701 tggggaatac tgttggtggc ggaaccgggc gttctctggc tactgcagca ggcgctgttg
     5761 caggtggcgt agctggtcag ggcgtacaga gtgcaatgaa caaaacgcag ggtgtcgagc
     5821 tggaaattcg taaagacgat ggtaatacca tcatggtggt acagaaacaa ggcaacactc
     5881 gtttctctcc gggccaacgt gtcgtactgg ccagcaatgg cagtcaggtg accgtttctc
     5941 cgcgctaaat aagtttgcgt gtggtcaggt tactgaccac acgccccctt catttcaccc
     6001 tttggcctgt aactcaatga tattatgctc aagttttgcg atgagcgtaa tcagttgctc
     6061 cagttcctct gcggagatgc catgtaatat ttccgcgcgg gttttgttaa taacagcttc
     6121 catttcgctg atcagtggct ctgccttttc cgtcagttta atacgtttag cccgacgatc
     6181 gctggcacaa gtttgacgcg aaattaaccc tttttcttcc agttggtcca gagtacggac
     6241 cagtgatggc tgctcgatgc caatcgcttt tgccagttga atttgcgact ggtctggagg
     6301 taactgatgg atattgtgta acgtaaccca atgggtttgt gttaactcca gcggtttcag
     6361 gcgatggtct atcagagcac gccatatgcg caccaaccgt gccagatcag aacctagtgg
     6421 cgattccaat ttcatctcct tataattagc ttgctaagat attatgcggc ttttagaata
     6481 gtgtgcagca attgtattgc taaaacaaat gtattgctgc atttggttac cgtcagacat
     6541 atttttcaga aattgcgcgt aaatttttcg cacttaaaga atatttatta atctaacgca
     6601 atatattcgg tcgtaaagga atctactttg tgaagtttat gctcaatgca acaggattgc
     6661 ccttacaaga tctggtgttc ggtgcgtccg tctattttcc tccgtttttc aaagcattcg
     6721 cgtttggatt cgtcatctgg cttgtcgtac accgcctgct tcgtggctgg atctacgccg
     6781 gtgacatctg gcatcccttg ttaatggatt tatcgctgtt tgcgatttgc gtttgccttg
     6841 ctctggcaat actgattgcg tggtaactat gtcaattaaa acaattaagt atttctcaac
     6901 aatcattgta gcggtagttg cggttcttgc cggatggtgg ctgtggaatt attacatgca
     6961 atctccgtgg acacgcgacg ggaaaatacg cgcagaacag gtttctatca ccccacaggt
     7021 gtcgggacgt attgttgagc tgaatataaa agacaaccag ctggttaacg caggggatct
     7081 tctgctcacg atcgataaaa cgccctttca gatcgccgag ctgaacgcac aggctcagct
     7141 ggcgaaagcg caatctgacc tcgcaaaagc caataacgaa gcgaatcgtc gtcgtcatct
     7201 ctcacaaaat tttatttctg ccgaagaact cgataccgct aacctcaatg ttaaagcgat
     7261 gcaggccagt gttaatgccg cacaggccac gctgaaacag acacaatggc aactggcgca
     7321 aacggaaatt cgcgccccgg tgagtggacg ggtgactaac ctcactaccc gcatcggcga
     7381 ttacgctgac accgggaaac cgctttttgc ccttgtcgac agccactcgt tttacgtcat
     7441 tggttatttt gaagaaacca agttgcgcca tatccgcgaa ggtgcaccag cacaaattac
     7501 cctctatagt gataacaaaa cgttacaggg tcacgtttcc agtatcggtc gggcgattta
     7561 tgatcagagc gttgaaagtg actcaagcct tattccggat gtgaaaccta acgtcccctg
     7621 ggttcgtctc gcccaacgcg ttcccgtccg ctttgcgctg gataaagttc caggcgatgt
     7681 cacgctggtg tcgggcacca cctgtagcat tgccgtaggt caataatgaa cgcatcgtaa
     7741 tggtccttgc gcaatttgcc ctggttcagg gccacgctgg cgcaatggcg ttatgcgtta
     7801 cgcaatacca ttgccatgtg tctggcgctg acggttgcct attatttgaa tctggatgaa
     7861 ccctattggg cgatgacctc ggctgcagtg gttagctttc ccaccgttgg cggtgtcatc
     7921 agcaaaagcc tcggacgcat cgctggcagt ttactgggag ccattgcggc actgcttctt
     7981 gccggacata cgctcaatga gccgtggttt tttctattga gcatgtcggc gtggcttggc
     8041 ttttgtacct gggcctgtgc gcacttcacg aataacgtcg cgtatgcatt tcaactggcg
     8101 ggctacacgg ctgccatcat cgcctttccg atggttaata ttactgaggc cagccagctg
     8161 tgggatatcg ctcaggcgcg cgtttgcgag gtgatagtcg gtattttgtg cggcggcatg
     8221 atgatgatga tcctgcctag cagttccgat gctactgccc ttttaactgc attgaaaaac
     8281 atgcacgccc gattactgga acatgccagt ttactctggc agcctgaaac aaacgatgcc
     8341 attcgtgcag cacatgaggg ggtgattggg cagatactga ccatgaattt gctgcgtatc
     8401 caggctttct ggagccacta tcgttttcgc cagcaaaacg cgcgccttaa tgcgctactc
     8461 caccagcaat tacgtatgac cagtgtcatc tccagcctgc gacgtatgtt gctcaactgg
     8521 ccctcaccgc caggtgccac gcgagaaatt ctcgaacaat tgctgactgc gctcgccagt
     8581 tcgcaaacag atgcttacac cgtcgcacgt attatcgccc cgctacgccc gaccaacgtc
     8641 gccgactacc ggcacgtcgc cttctggcag cgactacgtt atttttgccg cctttatctg
     8701 caaagtagtc aggaattaca tcgtctgcaa agcggtgtag atgatcatgc cagactcgca
     8761 cggacatccg ctctggctcg tcataccgac aacgccgaag ctatgtggag cgggctgcgt
     8821 acattttgta cgttgatgat gattggcgca tggagtattg catcgcaatg ggatgccggt
     8881 gccaatgcat taacgctggc agcaattagc tgcgtactct actccgccgt cgcagcaccg
     8941 tttaagtcgt tgtcactgct gatgcgcacg ctggtgttac tttcgctatt cagctttgtg
     9001 gtcaaatttg gcctgatggt ccagattagc gatctgtggc aatttttact gtttctcttt
     9061 ccactgctgg cgacaatgca gcttcttaaa ttgcagatgc ccaaatttgc cgcactgtgg
     9121 gggcaactga ttgtttttat gggttcgttt atcgctgtca ctaatccccc ggtgtatgat
     9181 tttgctgatt ttcttaacga gaatctggca aaaatcgttg gcgttgcgct ggcttggcta
     9241 gcgttcgcca ttctgcgtcc aggatcggat gctcgtaaaa gccgccgcca tattcgcgcg
     9301 ctgcgccggg attttgtcga tcagctaagc cgccatccaa cactgagtgg aagcgaattt
     9361 gaatcgctca cttatcatca cgtcagtcag ttgagtaaca gccaggatgc gctggctcgc
     9421 cgttggttat tacgctgggg tgtagtgctg ctgaactgtt ctcatgttgt ctggcaattg
     9481 cgcgactggg aatcgcgttc cgatccgtta tcgcgagtac gggataactg tatttcactg
     9541 ttgcggggag tgatgagtga gcgtggcgtt cagcaaaaat cactggcggc cacacttgaa
     9601 gaattacagc ggatttgcga cagccttgcc cgtcatcatc aacctgccgc ccgtgagctg
     9661 gcggcaattg tctggcggct gtactgctcg ctttcgcaac ttgagcaagc accaccgcaa
     9721 ggtacgctgg cctcgtaatt acttaattac accacaggca tagcgttcac cgccaccgcc
     9781 cagcggttta ggttgatcgg acatattatc gccgccaacg tggaccatca gcgccttgtc
     9841 tttgatttca tccagtgatt tcagacgagg cgcgatgacg gcatcggtag ctttgccgtc
     9901 attattgacg accagtgcag gcagatcgcc taaatgcccg gtaccttctg gcccttcatg
     9961 tttaccggta ttttgtggat caagatgccc gcctgcggat tccgcggcgc tggctttgcc
    10021 atctttggtg gctggctggc agcttccttt ggcatgaata tggaagccat gttcaccggg
    10081 gggtaatgct ttcagatcgg gtgaaaactc cagaccttta tcggtttcag taatggtgac
    10141 gctaccaatt gactgcccaa ccccttgcga cgtgacgagg ttcatctcga ctttttcact
    10201 ggcagcttgt gcgccggtcg caacaaccag cgccagaatg gccagactaa aacgtttcat
    10261 aggacctccg ttcattgtgc attcctgaat aagtgtaaac cagtgacaca tttttgaacg
    10321 gcggctattc ctaaaagtgc ttacggtacg tcgtacccca gtgccgcttt acggatacga
    10381 aaccattgtt gacgggtcat tttcagtgtt tctgcttcga cagctgcccg tacgcgctca
    10441 attttacctg agccgataat tggcagcggc tgcgacggta aacgtaatac ccaggcgtaa
    10501 accacctgtt caatcgagcc cgcgtttaac tcctccgcca ccacagccag ttcatcacgc
    10561 agcggctgga aataatcatc attaaacaga cgaccaccac caaggcagga ccacgccatc
    10621 ggacgaacac gcagttgttg tagttggtcg agcgtgccat ccagcagtaa cggctgatgc
    10681 accggggata tttccacctg attagtggca agggtaaacg gcagacgtga tttcaacagg
    10741 gcaaattgcg caggcgtaaa gttcgatacg ccaaaatgac gcactttgcc gctctgatgc
    10801 agatgtttga acgcgtccgc cacttcatcg gcatccatta acgggtctgg tcggtggatt
    10861 aacagcagat ccagatgatc ggtcgcgaga ttaattagcg actgttcggc gctcttaatg
    10921 atgtgatcgc ggtcagtgat gtaatgacca atgacgtttt cttcacgcgc ggtcgtcgcg
    10981 ataccgcatt tactgacgat ttccatccgt tcacgcaggt gaggtgccag tttcagtgcc
    11041 tcgccaaacg ccgcttcgca ctgatagcca ccataaatat cagcatggtc cacggtggtc
    11101 acgccgagat ccagatgctc ttcaataaaa ctgaccagct ggcgggcgga catattccag
    11161 tccatcaatc gccagtagcc catcacaaaa cgggaaaact ccgggccttg cggcgcaata
    11221 gtaatacgct gaaccataat cgcttcctct tatcagatat gagaggagta tacgcaagat
    11281 taggttcaaa agagtgatgg ttgctccggt tcgtctgatg acgctggctt atttgcgcgt
    11341 aatttgcgca ttaatcgctg ccgacaaagg cgcagcacct cttgtttttc gccatcgctc
    11401 attttattcc agttaaaacg ctcatcccga ctacgaaaac agccgcgaca aaacccgcgt
    11461 tcgtcagact ggcaaattcc ccggcacggg ctctggacgg gaaagaactc taattgctcc
    11521 gccacttcgc cctcctcaaa taagattatt accattattg aagctgttaa tgtccaaagt
    11581 agcaactttg cttgcactag accgactggt ctactacact ccaacgcatg aacaaacaca
    11641 ccgaacatga tactcgcgaa catctcctgg cgacgggcga gcaactttgc ctgcaacgtg
    11701 ggttcaccgg gatggggcta agcgaattac taaaaatcgc tgaagtgccg aaagggtcct
    11761 tctatcacta ctttcgctct aaagaagcgt ttggcgttgc tatgcttgag cgccattacg
    11821 ccacatatca ccagcgactg actgagttgc tgcaatccgg cgaaggtaac taccgcgacc
    11881 gcatactggc ttattaccag caaacactga accagttttg ccaacatgga accatcagtg
    11941 gttgcctgac agtaaaactc tctgccgaag tgtgcgatct gtcagaagat atgcgtagcg
    12001 cgatggataa aggcgctcgc ggcgtgattg ccctgctctc tcaggcgctg gaaaatggcc
    12061 atgagaacca ctgtttaacc ttttgtggcg aaccgctgca acaggcacaa gtgctttacg
    12121 cactatggct gggtgcgaat ctgcaggcca aaatttcgcg caattccgag ccactggaaa
    12181 acgcgctggc acatgtaaaa accattattg cgacgcctgc cgtttagcag gcatttttta
    12241 tcaccagacg accgggagcc tttatgtcat ctgaaaaact gtattcccca ctgaaagtgg
    12301 gcgcaataac tgcggcaaac cgtattttta tggcaccgct gacgcgtctg cgcagtattg
    12361 aaccgggtga catccctacc ccgttgatgg cggaatacta tcgccaacgt gccagtgccg
    12421 gtttgattat tagcgaagcc acgcaaattt ctgcccaggc aaaagggtat gctggtgcac
    12481 ctggcatcca tagtccggaa caaattgccg catggaaaaa aattaccgct ggcgttcatg
    12541 ctgaaaatgg tcatatggcc gtacagctgt ggcacactgg acgcatttct cacgccagcc
    12601 tgcaacctgg cggtcaggca ccggtagcgc cttcagcact tagcgcggga acacgtactt
    12661 ctctgcgcga tgaaaatggt caggcgatcc gtgttgaaac atccatgccg cgtgcgcttg
    12721 aactggaaga gattccaggt atcgtcaatg atttccgtca ggccattgct aacgcgcgtg
    12781 aagccggttt tgatctggta gagctccact ctgctcacgg ttatttgctg catcagttcc
    12841 tttccccttc ttcaaaccat cgtaccgatc agtacggcgg cagcgtggaa aatcgcgcac
    12901 gtttggtact ggaagtggtc gatgccggga ttgaagaatg gggtgccgat cgcattggca
    12961 ttcgcgtttc gccaatcggt actttccaga acacggataa cggcccgaat gaagaagccg
    13021 atgcactgta tctgattgaa caactgggta aacgcggcat tgcttatctg catatgtcag
    13081 aaccagattg ggcgggaggt gaaccgtata ctgatgcgtt ccgcgaaaaa gtacgcgccc
    13141 gtttccacgg tccgattatc ggcgcaggtg catacacggt agaaaaagcc gaaacgctga
    13201 ttggcaaagg gttaattgat gcggtggcat ttggtcgtga ctggattgcg aacccggatc
    13261 tggtcgcccg cttgcagcgc agagctgagc ttaacccaca gcgtgccgaa agtttctacg
    13321 gtggcggcgc ggaaggctat accgattacc cgacgttgta atccaacatt gcgagcggcg
    13381 taaagccgcc gctatactaa aacaacattt tgaatctgtt agccattttg aggataaaaa
    13441 gatgcgtctt cttcatacca tgctgcgcgt tggcgatctg caacgctcca tcgattttta
    13501 taccaaagtg ctgggcatga aactgctgcg taccagcgaa aacccggaat acaaatactc
    13561 actggcgttt gttggctacg gcccggaaac cgaagaagcg gtgattgaac tgacctacaa
    13621 ctggggcgtg gataaatacg aactcggcac tgcttatggt cacatcgcgc ttagcgtaga
    13681 taacgccgct gaagcgtgcg aaaaaatccg tcaaaacggg ggtaacgtga cccgtgaagc
    13741 gggtccggta aaaggcggta ctacggttat cgcgtttgtg gaagatccgg acggttacaa
    13801 aattgagtta atcgaagaga aagacgccgg tcgcggtctg ggcaactaat ctcctgccgg
    13861 gcgtgaactc atcgcgcccg catctttact gcatcgataa gtaatatttg tcataatgcg
    13921 cgctgcaatt tatccgtatt aagagaatca gatgtccgat aacgctcaac ttaccggtct
    13981 gtgcgaccgt tttcgtggtt tttatcctgt tgtgatcgat gttgaaacag ccggatttaa
    14041 cgccaaaacc gatgcgctgc ttgagattgc cgccatcacc ctgaaaatgg atgaacaagg
    14101 ctggctgatg ccggacacca cattacattt ccacgtcgaa ccatttgtcg gcgcaaattt
    14161 gcagccagag gccctcgcct tcaacggcat tgacccgaac gatcccgatc gcggcgcggt
    14221 cagcgaatac gaggcgctgc acgaaatttt taaagttgta cgtaaaggta ttaaagcgag
    14281 cggctgtaac cgcgccatta tggtggcgca caatgccaat tttgatcaca gctttatgat
    14341 ggccgccgca gaacgcgcct cactgaaacg taacccgttc caccctttcg ccacttttga
    14401 cactgctgca ctggccgggc tggcactcgg acaaaccgta ttgtcaaagg cttgccagac
    14461 cgctggcatg gacttcgaca gcacccaggc gcactccgcg ctgtacgaca ccgaacgcac
    14521 tgctgtgctg ttttgtgaaa tcgtcaaccg ctggaaacgt ctgggaggct ggccgctacc
    14581 tgccgccgaa gaggtgtaat cgagtcgatg cctgatgaca tgcaatgatt caggcatcta
    14641 tagtgaggct attccacgca tcaagcatga tattcacggg gaatagcgtt aatggcagat
    14701 aatccagacc cttcatcgct cctgccggac gtgttttcac tggcgacccg cgactggttt
    14761 cttcgcgcct ttaaacagcc gaccgctgtc cagtcgcaaa cctggcatgt ggcggcgcga
    14821 ggcgaacatg cgctggtgat tgcaccgact ggctccggga aaacgctggc agcatttctc
    14881 tacgccctcg atcggctctt ccgcgaaggc ggcgaagata cccgcgaggc gcataagcgt
    14941 aaaacctcac gcatcctcta tatttcaccg ataaaagccc tgggcaccga cgttcagcgc
    15001 aacttgcaga tcccgttgaa gggtattgcc gatgaacggc ggcggcgcgg cgaaacggaa
    15061 gtcaatcttc gcgtagggat ccgtactggc gatacgcctg cacaggaacg cagcaaactc
    15121 acccgtaatc cgccggatat tctgatcacc acacccgaat cactctatct gatgctgacc
    15181 tcccgcgcgc gcgaaacgct acgcggcgtc gaaacggtaa ttattgatga agtccacgcg
    15241 gtagcgggca gtaaacgtgg tgcgcatctg gcgttaagtc tggagcggct cgatgcgctg
    15301 ctccacacct cagcacagcg aattggcctt tctgccactg tgcgctcagc cagcgatgtg
    15361 gcagcatttc ttggtggcaa tcgcccggtt acggtagtca acccgcccgc aatgcgccat
    15421 ccgcagatac gaattgtcgt accggtcgcc aatatggatg atgtctcatc ggtcgccagc
    15481 agcaccggcg aagacagcca tgccggccgg gaaggctcca tctggccata tattgaaacg
    15541 ggtatccttg atgaagtgtt gcgccatcgc tcgaccattg tctttactaa ttcacgtggg
    15601 ctggcggaaa aactgacggc acgattaaat gagctttacg ccgcacgctt acagcgttcc
    15661 ccgtctatcg ccgttgatgc ggcccatttc gagtcgacct ccggcgcaac ctctaaccgt
    15721 gtacaaagta gcgacgtttt tattgcccgc tcacaccacg gctccgtctc taaagaacaa
    15781 cgagcaatca ccgagcaggc gctgaaatcg ggtgagttac gctgcgtggt cgcaacctcc
    15841 agtctcgaac tggggattga tatgggcgca gtggatctgg tgattcaggt ggcaacgccg
    15901 ctttctgttg ccagtgggtt acaacgcatt ggtcgcgccg gacatcaggt tggcggtgta
    15961 tctaaagggc tgtttttccc tcgtacccgg cgtgacttag tcgattccgc agtcattgta
    16021 gagtgtatgt tcgcaggcag gctggaaaac ctgacaccgc cgcataatcc actcgacgtc
    16081 cttgcgcagc aaaccgttgc cgccgcggcg atggatgcat tacaggtaga cgaatggtac
    16141 tcccgcgtac gccgtgccgc accgtggaaa gatctgccaa gacgtgtttt tgacgctacg
    16201 ctggatatgc tttccgggcg ctatccctct ggcgattttt ctgcttttcg ccccaaactg
    16261 gtctggaacc gggagaccgg gatattgacc gcccgacctg gcgctcaatt gttggcggtt
    16321 accagcggtg gcaccattcc ggatcgtggc atgtatagcg tgttattacc cgaaggtgaa
    16381 gaacaggccg gttcgcggcg ggtgggtgaa ctggatgagg agatggtata tgagtcgcgg
    16441 gtgaacgaca ttatcactct cggcgctacc tcatggcgga tccagcaaat cacccgcgat
    16501 caggtgattg tgactcctgc tccgggtcgt tctgcccggc tccccttctg gcgtggtgaa
    16561 ggtaacggac gtccggctga attaggcgag atgatcggcg attttcttca tttgctggcg
    16621 gatggcgcgt tcttttccgg gactattccc ccgtggctgg cagaagaaaa tacgatcgcc
    16681 aatattcagg ggttgattga cgagcaacgc aacgcgacgg gcatcgttcc ggggagtcgc
    16741 catctggtcc tcgaacggtg ccgtgatgaa attggcgact ggcgtattat tttgcactct
    16801 ccctatggaa gacgggtgca tgaaccctgg gcgctggcga ttgccgggcg aatacatgcc
    16861 ctatggggcg ctgacgcgtc tgtggtcgcc agtgatgacg gcattgttgc gcgcattcct
    16921 gacactgatg gtaaattgcc cgacgccgcg atttttttgt ttgaaccaga aaagttgctg
    16981 caaattgtcc gcgaggcggt aggcagctcg gcacttttcg ccgcccgttt tcgcgaatgc
    17041 gccgcgcggg cattattaat gccggggcgc actccgggcc atcgcacccc gctttggcaa
    17101 caacggctgc gcgccagtca gttgctggaa atcgctcagg gatatccgga ttttccggtc
    17161 attctcgaaa ccctacgcga atgtctgcaa gatgtttatg atcttcccgc actggaacgt
    17221 ttgatgcgtc gcctgaacgg tggcgaaatt caaatatccg atgtaacgac cactacgccc
    17281 tcgcctttcg ccacaagttt attgttcggc tatgtcgcgg aatttatgta ccagagcgac
    17341 gccccgctgg cagagcgccg ggcatccgta ctgtcgctgg acagcgagtt actgcgcaat
    17401 ctactcggac aggtcgatcc gggggaatta ctcgacccgc aggtcattcg ccaggtggaa
    17461 gaagagttgc aacgactggc tcctggcaga agagcgaaag gtgaagaagg attgttcgac
    17521 ctgctgcgcg aactggggcc aatgaccgtt gaagacctgg cgcaacggca tacaggcagc
    17581 agtgaagagg ttgcgtcgta tctggaaaat cttcttgcag taaaacgaat cttcccagcg
    17641 atgattagcg gacaggagcg tcttgcctgt atggatgatg ccgccaggct gcgtgatgcc
    17701 ctcggcgtac gactaccaga gtcattgcca gagatttatt tacatagagt cagttacccg
    17761 cttcgcgacc tctttctgcg ctatctccgg gctcatgctc tggtcacggc tgaacaactg
    17821 gctcatgagt ttagtctcgg tattgccatt gtcgaagagc agcttcagca actgcgtgaa
    17881 cagggtctgg tgatgaatct gcaacaagac atctgggtga gcgatgaagt atttcgtcgt
    17941 ctgcgtttgc gctcgctgca agccgccaga gaagcgacgc gtcccgttgc agccacgacc
    18001 tatgcgcgat tgctgctgga acgtcagggc gtattacccg ccaccgatgg tagcccggcg
    18061 ctctttgcct caacatcgcc aggcgtttat gagggcgtag atggcgtgat gcgggtgatc
    18121 gaacagcttg ccggagtcgg tttacccgcc tcactctggg aaagccagat cctgcctgcc
    18181 cgcgtacgcg actattcgtc agaaatgctc gatgaattac tggcaaccgg tgcggttatc
    18241 tggtcggggc aaaaaaactg ggtgaagatg acggcctggt ggcactgcat ctacaggaat
    18301 atgctgcaga atcgttcact cccgccgaag cggatcaggc gaatcgttcg gcgctgcaac
    18361 aagcgatagt cgctgttctg gctgacggag gagcctggtt tgcacaacaa atcagccaac
    18421 ggatacgcga caaaatcggc gaatcggttg atctctctgc cctgcaagag gcgttatggg
    18481 cgctggtctg gcaaggcgtc atcaccagcg acatttgggc accgttacgc gccctcaccc
    18541 gcagcagttc caacgcacgc acctcaactc gccgcagtca ccgggctcgt cgtggacgtc
    18601 ctgtctatgc gcaacccgtc tcgccgctgg tatcttacaa cacaccaaat ctggctggac
    18661 gctggccgtt attgcaggtg gagccactaa acgataccga aaggatgctg gcgctggcgg
    18721 aaaatatgct cgaccgctac ggcatcatca gtcgtcaggc ggtgatagcc gaaaatatcc
    18781 ctggcgggtt tccatcgatg caaacgcttt gtcgaagtat ggaagactcc gggcgaatta
    18841 tgcgaggtcg ttttgtagaa ggtctgggtg gcgcgcaatt cgctgaacgt ctgactattg
    18901 accgattgcg cgatctggcg acacaagcca cgcaaacgcg ccactataca ccagtggcgc
    18961 tctctgccaa cgatccggct aatgtgtggg gaaatcttct gccctggcct gcacatccgg
    19021 caacgctggt tccaacgcgt cgggcgggtg cgctggtggt cgtttctggc ggcaaattgt
    19081 tactctatct ggcgcaaggt ggcaaaaaaa tgctggtctg gcaggaaaaa gaggaattac
    19141 tcgccccaga ggttttccac gcgctgacta ccgcactgcg tcgcgaacca cggctgcgct
    19201 ttacgctaac agaagtgaat gatctaccgg tccggcaaac gccgatgttt acgctgctgc
    19261 gtgaggcggg attttcaagt tcgccacaag ggctggattg gggatagaga aaggactgac
    19321 ggatgcccgt tcgcatccgt cagtattgca ggacggatta ttccgcgtcc ggctcttcag
    19381 acttgtattt agcggcagtt tctttgatca gctgctgcag ttcgccacgc tgatacattt
    19441 cgatcacgat atcacaaccg ccgaccagct tgccgtcaac ccacagttgc gggaaggtcg
    19501 gccagttagc atatttcggc agttccgcac gaatgtccgg attctgcaga atatcaacat
    19561 aggcaaaacg ttcgccacat gcggcaagcg cctggactgc ctgggcagag aaaccgcagc
    19621 tcggcagttt cggtgaacct ttcatgtaca gcaggatcgg gttttcagca atctggcgtt
    19681 ggattttttc gatagtggtg ctcattgtct tgcttcctca aacttcttta cggcagtaat
    19741 ctgacattgt agcgggtcag tgcggcatcg gaaaataaca ttttcatcac gcttttgcta
    19801 ttttatccct ttgctctatt tgttgcattt caaatattcg tttcttctct gatgccggca
    19861 aactggcgta tttataacca tttttagctt tcactgctgc atttttttgc gctcgccaac
    19921 gaaacgtatt ttttaacaat aaaagctatt aactttctct tcttctatgc attagaatca
    19981 tcaagttttg taaatcagac gcaggcatga tagacctgcc ttaacagagg gacgctcagt
    20041 ggcgcggata aaccgtattt cgatcacgct ctgtgcttta ctttttacca ccctgccttt
    20101 aacgcctatg gcccatgctt caaagcaagc cagggagagt tctgctacca ctcatatcac
    20161 caaaaaagca gataaaaaga aaagcacggc aaccaccaaa aagccccaga aaacagcgaa
    20221 aaaagccgcc agtaaaagta cgaccaaaag caaaaccgct tcttccgtta aaaaatcttc
    20281 cattaccgct tctaaaaacg ccaaaactcg cagcaaacac gccgtcaata aaacggcctc
    20341 agccagcttt accgaaaagt gtaccaaacg taagggctat aaatcgcatt gtgtgaaagt
    20401 caaaaatgcc gcgtcaggaa ctcttgccga cgcgcacaaa gcgaaggtgc aaaaagctac
    20461 gaaagtggca atgaataaac tgatgcagca aattggtaag ccatatcgtt ggggtggcag
    20521 ctcaccgcgt accggttttg attgcagcgg cctggtttat tacgcttata aagatttggt
    20581 gaaaattcgt attccgcgta cggcgaatga aatgtatcac ctgcgtgatg cagcgccaat
    20641 cgaacgtagt gaactgaaaa acggcgacct ggtctttttc cgtactcagg gacgcggcac
    20701 agccgatcat gtcggcgtgt atgtcggcaa cggcaaattt attcagtcac cgcgcacagg
    20761 tcaggaaatt caaatcactt ctctcagtga agactactgg cagcgccact atgttggcgc
    20821 tcgtcgggta atgaccccaa aaacacttcg ctaaaacttt accctgttgt tacggcaaca
    20881 gggtaagttc atcttttgtc tcacctttta atttgctacc ctatccatac gcacaataag
    20941 gctattgtac gtatgcaaat taataataaa ggagagtagc aatgtcattc gaattacctg
    21001 cactaccata tgctaaagat gctctggcac cgcacatttc tgcggaaact atcgagtatc
    21061 actacggcaa gcatcatcag acttatgtca ctaacctgaa caacctgatt aaaggtaccg
    21121 cgtttgaagg taaatcactg gaagagatta ttcgcagctc tgaaggtggc gtattcaaca
    21181 acgcagctca ggtctggaac catactttct actggaactg cctggcaccg aacgccggtg
    21241 gcgaaccgac tggaaaagtc gctgaagcta tcgccgcatc ttttggcagc tttgccgatt
    21301 tcaaagcgca gtttactgat gcagcgatca aaaactttgg ttctggctgg acctggctgg
    21361 tgaaaaacag cgatggcaaa ctggctatcg tttcaacctc taacgcgggt actccgctga
    21421 ccaccgatgc gactccgctg ctgaccgttg atgtctggga acacgcttat tacatcgact
    21481 atcgcaatgc acgtcctggc tatctggagc acttctgggc gctggtgaac tgggaattcg
    21541 tagcgaaaaa tctcgctgca taataactga tggcaaatgc agcattgcct gatgcgcttc
    21601 gcttaccatg ccaacgcgga tgaggcgtta acagcgcatt cggcaagata aatcgcacgt
    21661 tgtcagcaac tgtaacgcag aaggttatcc ttctgcgttt ttgtttaatt agctgttggc
    21721 aacgcaaact gtttcaggtt gttttctggc tgacataaac accagcaata atgccagtcc
    21781 cgcgacaatc gcccccatca ccggcacaaa gctgtatccc agcccagcgg aaattaccgc
    21841 accaccagcc gctgctccca gcgcatttcc aagattaaag gcaccaatat tgactgatga
    21901 agacagacct ggcgcttcac tggcgacacg catcacgcgc atctgtaacg gcggtacgac
    21961 cgcaaaggtt gctgcgcccc acaccaccat gctaatagct gcgccgaact cattgcgggc
    22021 caggaacggg attgccagca taatcaccat cagcagcaac aaaaagcctt tcaacgtgcc
    22081 gttaactgaa cgatctgcca gtttgccgcc gagatagtta ccgatagaga atccgacacc
    22141 aatcagcacc agcattgccg tgacgaacac cggtgttgcg tgggtaatac tttgcagtac
    22201 cggagagata taggtgtaga gagtaaacat tgcgccagct cccagtaccg tcgtcagcaa
    22261 tgcagacagc acctgcggac gcattaatac cgccagctct tttttcactt caggtcgtgc
    22321 ccctgtacca cctttaggta atgagaagaa cagacttacc attgaaatca ctcccagccc
    22381 cgccgttgcc agaaatgaca tccgccagcc gatggtttca cccaaccagg tcgccgccgg
    22441 cacgccaccg atatttgcca gggttaaccc cataaacata gtggcaactg cgctggcctg
    22501 tttatgtttt ggcaccacgc ttgcggccac gactgaaccc aaaccaaaaa atgctccgtg
    22561 attcaggctg gtcaaaatgc gtgaaagcat cagggtcata taatccggcg cgatggcgga
    22621 aagtacgttg ccgagcgtga aaattgccat caggaaaatc aacgcactgc ggcgggcacg
    22681 atgagaaagt agaagcgtca tcagcggcgc gcccaccatt acgccaactg cataggcact
    22741 gattaacatt ccggcagcgg gaatcgagac atccacaccg cgcgcaatga cgggcaacaa
    22801 gcccattggc gagaactccg ttgtcccgat accaaacgcg ccaatcgcca gcgccagcaa
    22861 cggatagtca attttcatgc cttatctcca cctcttcgcg tcattacgcg atattcatta
    22921 aagtggcgaa agcatgacag caatcacaaa aaaatgaaaa taacaaaaag agaaaacact
    22981 tttgccattt tgctaacaaa caggaaggag atgcgaggga gaacgcgctc cctcgagagg
    23041 aaatcagtgc agcgcggcag tcaaacccac ggctacgatc aaaccgagga cgataatcgt
    23101 tgttaccagt gaaaatttaa ggtcggtgct catcaagttt tctccttttt tattaccaca
    23161 caaaaagtga tattacgcat ttttacacac tgtgatgaaa aaatctcccg tcatttataa
    23221 tgataagtgt ttttaccact tccccttttc gtcaagatcg gccaaaattc cacgcttaca
    23281 ctatttgcgt actggccatt gaccccttcc tgacgctccg tgtcgttttt ccggcgtacc
    23341 gcaacacttt tgttgtgcgt aaggtgtgta aaggcaaacg tttaccttgc gattttgcag
    23401 gagctgaagt tagggtctgg agtgaaatgg aatggcaaca ataaaagatg tagcgaaacg
    23461 agcaaacgtt tccactacaa ctgtgtcaca cgtgatcaac aaaacacgtt tcgtcgctga
    23521 agaaacgcgc aacgccgtgt gggcagcgat taaagaatta cactactccc ctagcgcggt
    23581 ggcgcgtagc ctgaaggtta accacaccaa gtctatcggt ttgctggcga ccagcagcga
    23641 agcggcctat tttgccgaga tcattgaagc agttgaaaaa aattgcttcc agaaaggtta
    23701 taccctgatt ctgggcaatg cgtggaacaa tcttgagaaa cagcgggctt atctgtcgat
    23761 gatggcgcaa aaacgcgtcg atggtctgct ggtgatgtgt tctgagtacc cagagccgtt
    23821 gctggcgatg ctggaagagt atcgccatat cccaatggtg gtgatggact ggggtgaagc
    23881 aaaagctgac ttcaccgatg cggtcattga taacgcgttc gaaggcggct acatggccgg
    23941 gcgttatctg attgaacgcg gtcaccgcga aatcggcgtt atccccggcc cgctggaacg
    24001 taacactggc gcaggccgcc ttgtcggttt tatgaaggcg atggaagaag caatgatcaa
    24061 ggtgccggaa agctggattg tgcagggtga ctttgaacct gaatccggtt atcgcgccat
    24121 gcagcaaatc ctgtcgcagc cgcatcgccc tactgccgtc ttctgtggtg gcgatatcat
    24181 ggcaatgggc gcactttgtg ctgctgatga aatggggctg cgcgtcccgc aggatgtttc
    24241 gctgattggt tatgataacg tgcgcaacgc ccgctatttt acgccggcgc tgaccacaat
    24301 ccatcagcca aaagattcgc tgggtgaaac agcgttcaac atgctgttgg atcgtatcgt
    24361 caacaaacgt gaagaaccgc agtccattga agtgcatccg cgcttgattg aacgccgctc
    24421 cgtggctgac ggcccgttcc gcgactatcg tcgttaatca cccgttgcgg gagtctcttc
    24481 cggctcccgc agccactcct tattcagcgt ctcactatcg ccgagatact caagcaacca
    24541 ggttaacgca ggcgacatat cattttgctg ccatgtcaga caacatgccg aatccggaaa
    24601 ggggttttcc agttcaagtg ctacccactt cccctcatta agccacggtt tggcgaaatg
    24661 tgttggcacc atccctatgc ataatcctgc cgagatacag gttgccgatg attcccagtc
    24721 aggcacgaca actctttttt ggttatccag caaccaggta atacgtttag gtagcgttcg
    24781 cgaggtgtct tcgcgcacca acgacggcca gttgcgcaac gtatcatcgc tgaacgggcc
    24841 atccatcaac gccagcgggt gatggctggc aacaacgcaa ctccagctta gcatccccat
    24901 atcccggaag gcataacgac cgcctaccgg aatcgcgcgt gttgcgccaa tcgccagttc
    24961 cacgcgcccg tcggaaagcg catcccagac accgttgaac acttcctgaa agacaagaag
    25021 ttcgacatca tcaaaatggc gataaaaatc aacgatcatc tgccgtgtac gttctggcct
    25081 gacaatatta tccactgcga tagctaactg accgcgccag ccgttcgcta tctgctgaca
    25141 ttgctggcgg gtgatctgca tttttttgac aacagagcgc ccttctttga gaaaccacgc
    25201 tccagcagcg gtcagctcca catcacggtg ccgtcgttca aagagcggca ccgccagcca
    25261 ctcttccagc tgacgcacgg tatagctgac cgcagaagga acgcgatgca gctcctgtgc
    25321 cgcagcgcta aaactaccat tacgcgctac cgcatcaaca acttcgagtg aatattctga
    25381 ccacatagtc tgcctgcaaa atttttgaaa ccaatcatca aatattaccg tttcacaaca
    25441 ctaatttcac tccctacact ttgcggcggt gtttaattga gagatttaga gaatatacat
    25501 gcaacctggg aaaagatttt tagtctggct ggcgggtttg agcgtactcg gttttctggc
    25561 aaccgatatg tatctgcctg ctttcgccgc catacaggcc gacctgcaaa cgcctgcgtc
    25621 tgctgtcagt gccagcctta gtctgttcct tgccggtttt gccgcagccc agcttctgtg
    25681 ggggccgctc tccgaccgtt atggtcgtaa accggtatta ttaatcggta tgacaatttt
    25741 tgcgttaggt agtctgggga tgctgtgggt agaaaacgcc gctacgctgc tggtattgcg
    25801 ttttgtacag gctgtgggtg tctgcgccgc ggcggttatc tggcaagcgt tagtgacgga
    25861 ttattatcct tcacagaaag ttaaccgaat ttttgcgacc atcataccgc tggtgggtct
    25921 atctccggcc ctggctcctc tgttaggaag ctggctgctg gtccattttt cctggcaggc
    25981 gattttcgcc accctgtttg ccattaccgt ggtgctgatt ctgcctattt tctggctcaa
    26041 acccacgacg aaggcccgta acaatagtca ggatggtctg acctttaccg acctgctacg
    26101 ttctaaaacc tatcgcggca acgtgctgat atacgcggcc tgttcagcca gtttttttgc
    26161 atggctgacc ggttcaccgt tcatccttag tgaaatgggc tacagcccgg cagttattgg
    26221 tttaagttat gtcccgcaaa ctatcgcgtt tctgattggt ggttatggct gtcgcgccgc
    26281 gctgcagaaa tggcaaggca agcagttatt accgtggttg ctggtgctgt ttgctgtcag
    26341 cgtcattgcg acctgggctg cgggcttcat tagccatgtg tcgctggtcg aaatcctgat
    26401 cccattctgt gtgatggcga ttgccaatgg cgcgatctac cctattgttg tcgcccaggc
    26461 gctgcgtccc ttcccacacg caactggtcg cgccgcagcg ttgcagaaca ctctacaact
    26521 gggtctgtgc ttcctcgcaa gtctggtagt ttcctggctt attagtatca gcacgccatt
    26581 gctcaccacc accagcgtga tgttatcaac agtagtgctg gtcgcgctgg gttacatgat
    26641 gcaacgttgt gaagaagctg gctgccagaa tcatggcaat gccgaagtcg ctcatagcga
    26701 atcacactga tctatatcga tatacttata cttaggctgc taacaaaatt ttgttgtatg
    26761 attgaaatta gcggcctata ctaatttcga gttgttaaag ctacgataaa tattatgttt
    26821 ttacggggac aggatcgttc ccgactcact atggatggtc atttcggcaa gggttcctcc
    26881 tttccctctg ttctacgtcg gattatagac tcgcggtttt ttctgcgaga tttctcacaa
    26941 agcccaaaaa gcgtctacgc tgttttaagg ttctgatcac cgaccagtga tggagaaact
    27001 atgagttcat cgtgtataga agaagtcagt gtaccggatg acaactggta ccgtatcgcc
    27061 aacgaattac ttagccgtgc cggtatagcc attaacggtt ctgccccggc ggatattcgt
    27121 gtgaaaaacc ccgatttttt taaacgcgtt ctgcaagaag gctctttggg gttaggcgaa
    27181 agttatatgg atggctggtg ggaatgtgac cgactggata tgttttttag caaagtctta
    27241 cgcgcaggtc tcgagaacca actcccccat catttcaaag acacgctgcg tattgccggc
    27301 gctcgtctct tcaatctgca gagtaaaaaa cgtgcctgga tagtcggcaa ggagcattac
    27361 gatttgggta atgacttgtt cagccgcatg cttgatccct tcatgcaata ttcctgcgct
    27421 tactggaaag atgccgataa tctggaatct gcccagcagg cgaagctcaa aatgatttgt
    27481 gaaaaattgc agttaaaacc tgggatgcgc gtactggata ttggctgcgg ctggggcgga
    27541 ctggcacact acatggcatc taattatgac gtaagcgtgg tgggcgtcac catttctgcc
    27601 gaacagcaaa aaatggctca ggaacgctgt gaaggcctgg atgtcaccat tttgctgcaa
    27661 gattatcgtg acctgaacga ccagtttgat cgtattgttt ctgtggggat gttcgagcac
    27721 gtcggaccga aaaattacga tacctatttt gcggtggtgg atcgtaattt gaaaccggaa
    27781 ggcatattcc tgctccatac tatcggttcg aaaaaaaccg atctgaatgt tgatccctgg
    27841 attaataaat atatttttcc gaacggttgc ctgccctctg tacgccagat tgctcagtcc
    27901 agcgaacccc actttgtgat ggaagactgg cataacttcg gtgctgatta cgatactacg
    27961 ttgatggcgt ggtatgaacg attcctcgcc gcatggccag aaattgcgga taactacagt
    28021 gaacgcttta aacggatgtt tacctattat ctgaatgcct gcgcaggtgc tttccgcgcc
    28081 cgtgatattc agctctggca ggtcgtgttc tcacgcggtg ttgaaaacgg ccttcgagtc
    28141 gctcgctaaa ggctattcta tcgccccctc tccgggggcg atttcagatc aggcttctgt
    28201 acctggttga ttcatggcat tttctcgtgc cgccagcaca cgttctaccg tatctaccac
    28261 tgcctgagtt tgtggatcaa tttcaatgtt gacgcgtgcg ccgagttttt tcttcccaag
    28321 agtcgtgcgt tccagagttt ccggaattaa atggacgcaa aaacgcgttg gcgtgacttc
    28381 gccgacggtc aggctaatac cgtcgatgcc aataaatcct ttgtacagga tatatttcat
    28441 caactgacta tcctggactt taaaccagat ctggcgatta ttttccgagg ttaatatttt
    28501 cgccacttca gcggtggtca taatatgacc tgacattaag tgtccgccaa tttcatcgct
    28561 gaattttgcc gcacgctcaa cgtttaccca atcccccact tttaaatcgc caagattggt
    28621 aatgcgtaac gtttctttca tcaggtcaaa actgacatgg ttgccgttaa tttccgtcac
    28681 ggtcaggcag caaccgttat gcgccacgga agcacctgtt tccaggccgt ccagcatgtg
    28741 gtcgggtaac tccaccacat gcgtacgaaa atttggtttc tcgtcaatcg acaccagttt
    28801 tgcggtgccc tgtacaatcc ccgtaaacat acttacaact cctgaaatca gttaagacat
    28861 tctgttcagc acaatagcag gtggaaaacg cccttaccag tgaaggggta agaatgacta
    28921 ttttttcact ggagaattaa taaatcctcg ctacaataga ctgaatttcc cctgcttctt
    28981 ctttttgctg cccattcagg cggcttttta gtctctcata taactacaaa taaaaggtgt
    29041 tcacgtgcag aagtatatca gtgaagcgcg tctgttatta gcattagcaa tcccggtgat
    29101 tctcgcgcaa atcgcccaaa ctgcgatggg ttttgtcgat accgtgatgg cgggcggcta
    29161 tagtgccacc gacatggcgg cggtcgctat cggtacttct atctggcttc cggcgatcct
    29221 ctttggtcac ggactgctgc tggcattaac gccggttatc gcgcaattaa atggttccgg
    29281 tcgacgtgag cgcattgcgc atcaggtgcg acaaggtttc tggctggcag gttttgtttc
    29341 cgttctcatt atgctggtgc tgtggaatgc aggttacatt atccgctcca tggaaaacat
    29401 cgatccggct ctggcggaca aagccgtggg ttatctgcgt gcgttgttgt ggggcgcgcc
    29461 aggctatctt ttcttccagg ttgcccgtaa ccagtgtgaa ggtctggcaa aaaccaagcc
    29521 gggtatggta atgggcttta tcggcctgct ggtgaacatt ccggtgaact atatctttat
    29581 ttatggtcat ttcggtatgc ctgagctcgg tggcgttggt tgtggcgtgg ctactgcggc
    29641 ggtgtattgg gttatgttcc ttgccatggt ttcttacatt aaacgcgccc gctccatgcg
    29701 cgatatccgt aacgaaaaag gcaccgcaaa acccgatcct gcggttatga aacgactgat
    29761 tcaactcggt ttgccgattg cgctggcact attctttgaa gtgacactgt ttgccgtcgt
    29821 ggctctgtta gtgtctccgc tcggtattgt tgatgtcgca ggacaccaga ttgccctgaa
    29881 ctttagttca ctaatgttcg tgcttccaat gtcgctggcg gcagcggtaa ctatccgcgt
    29941 aggttatcgt ctgggtcagg gctcaacgct ggatgcgcaa accgctgctc ggaccgggct
    30001 tatggtgggt gtctgtatgg caaccctgac ggccattttc acggtttcac tgcgggagca
    30061 aatcgccctg ttgtacaacg acaatcccga ggttgtaacg ctggctgcgc atttgatgtt
    30121 gctggcggcg gtatatcaga tttctgactc aatccaggtg attggcagtg ggattttgcg
    30181 tggttataaa gatacgcgtt ccattttcta tatcaccttt acagcttact gggtgctggg
    30241 cttgccaagc ggctatattc tggcactgac cgatctggtc gttgaaccta tggggccagc
    30301 aggcttctgg ataggcttta ttatcggcct gacatcggca gccattatga tgatgctgcg
    30361 tatgcggttc ctgcaacgta tgccgtcagc catcattctg caacgagcgt cccgctaata
    30421 aagacaaggc gcaaccttca cgggttgcgc ctgtgttttt acgcaggctg gagcgttgcg
    30481 ccaatcccgt cttcgtctgg ctgtaatttc agagcgttac acagagtttg ccgactggca
    30541 tctgttatct caacgcgtat atccccgtta acttcccctt tcacattgat gaagtcaggt
    30601 gaaatttctt tcgcactggt ttgcaggcaa atcgctccct ctccgcgcag gcgtttcact
    30661 ttcagttgca catgcgttgc cgcaccagtg atcagccaca gttttccgcc attcagattg
    30721 atgttaccaa caatgaaagt gacgctgtca ccttttaccg tactgccgtc gagaatcaac
    30781 acaccgccct gctgtacagt aatcgttgat tgttgcagct ctgcgccata accaataatc
    30841 attgaagcgc cgtccagtaa tgcaacactc ccggtcagtt caaccggtgc gcgatcttcc
    30901 tctacgacaa aattttcgtc cgcgctggtg ggtgacaaca ggatcactgt tccaccttgt
    30961 attaccgcat tttccagcgt accggcatgc acttcagccc gcccccccac attcaccgac
    31021 aggttttgcg tcttaccgga actgtagagc tgaaggccat ccgtccccag acgataaatg
    31081 gatccattct caacaatagt gtcagagtct tcaccaccga gattattcat ggtgccacca
    31141 gcaagcaacg tatcactggc tttcccggcc agcataaaaa cgccgccctg attaatctca
    31201 caattgctgg cttctccact tggatacacc aacaattcac caccttcatc taccacgacc
    31261 gctgaggttg tcccattaac caacaggccg ccctcttgat cgagaatgat tttttccgcg
    31321 cgatgtcctt ccagtacacg caggttaccg ccattttcca gcaacaaacc gcaggcataa
    31381 ccctgatcaa cgctgaattc accttcggga tggcggccat taaccgtagc gagcgtagag
    31441 agactaattg cgcctccctc ggactgaaca atatctgtgg caatggcacc gtcgctgacc
    31501 gatagcgtgc cattgttacc gtactgtgtg catttggcga tcccaccggt tgccactttc
    31561 aaccagccag cccccgtgac cacggtattt tcggctcttc cgccagcttc aacaatcaac
    31621 tcgccttcag taagttgcgt cgcgccaatg acctcgccgg tcttaacaat ttgcacattc
    31681 ccgtcgctca tcaagttttt cgcatcagat cccatgattt attcctttgc tgcatctgtg
    31741 tgcctttatt gctacctaag tgtaaaggct acggaggatt tatccacgac agatttgaga
    31801 tggtggcaaa caattctgtt taaactctga tacacgaatt attgggttgt atcagatgta
    31861 aatgcgatcc tgaataaaaa tcgcccttgc aaatcaacaa aatatagcca atttgcttaa
    31921 aagctctgca aacggtcgaa atagcgcaga aaattacgtt ttgcctcttg ccacctttcc
    31981 actctgccgc taatatttgt ccccgttgtc acctacaacg ttgcgttcat agctcagttg
    32041 gttagagcac caccttgaca tggtgggggt cggtggttcg agtccaattg aacgcaccat
    32101 cctgcgtccg tagctcagtt ggttagagca ccaccttgac atggtggggg tcggtggttc
    32161 gagtccactc ggacgcacca gattttctta atctggtctt ttcctttttc cctctgttta
    32221 ttctctgtat ccaatacgtt aaaagattta cactgtcttc gtatgcgtta tcagaaggag
    32281 aatcgctatg gcaactttgt tacaacttca ttttgctttt aatggcccat ttggcgacgc
    32341 aatggctgag cagcttaaac cacttgctga gtcgattaat caggaacctg gttttctgtg
    32401 gaaggtatgg acagaaagtg aaaagaacca cgaagccggt gggatctacc ttttcactga
    32461 tgaaaaaagc gcccttgcct atctggaaaa acatactgcc cgactgaaaa atctcggcgt
    32521 tgaggaagtt gtcgccaaag ttttcgatgt caatgagcca cttagtcaaa tcaatcaggc
    32581 aaaactcgcc tgacagaatt taatcaaggg cggttagcgc ccttttcatc cctgtctgaa
    32641 atttctcaaa ttctaaaaat ctcaaccaaa cttatctgat aacactaaat tcgaaagaat
    32701 gcgtacaggt aagtaacaat gaaaaaaatt gctattgtgg gtgccgggcc tacggggatc
    32761 tacaccttat tctcgcttct acagcaacaa actccacttt ctatttctat cttcgagcag
    32821 gctgacgagg ccggtgtcgg gatgccatac agtgatgagg aaaactcaaa aatgatgctg
    32881 gcaaatattg ccagtattga aataccgccg attaattgta cgtatctcga atggctacaa
    32941 aagcaagaag ccagccatct ccagcgttat ggcgttaaaa aagaaacctt gcacgatcgt
    33001 cagtttttac cgcgaattct gctgggcgaa tatttccgcg atcaattttt acgattagta
    33061 gaccaggcac gacagcaaaa atttgcagtg gctgtttatg aatcatgcca ggttaccgat
    33121 ctgcaaatta caaatgctgg cgtcatgctc gctacaaatc aggatttacc cagcgagacg
    33181 tttgatttag cggtgattgc cacgggtcac gtctggcctg atgaagaaga agcaaccaga
    33241 acgtattttc ccagcccgtg gtcaggcctg atggaagcaa aggtcgatgc gtgtaacgtg
    33301 ggtattatgg gaacatcctt gagcggactg gatgcggcaa tggcagtggc tattcagcat
    33361 ggttcgttca ttgaagatga taaacaacac gtcgtttttc accgcgataa cgcaagtgaa
    33421 aagctaaata tcacgttgat gtcgcgcacg ggtattttac ccgaagccga tttctattgc
    33481 cctattccct acgagccctt acacatcgtc accgatcagg cattaaatgc tgagattcaa
    33541 aaaggcgaat atggcctttt ggatcgggta tttagattga tagtagagga aatcaagttt
    33601 gctgatccag actggagcca acgcatagcc ttagagagcc tgaatgtcga ttcctttgct
    33661 caagcctggt ttgccgagcg caaacaacgc gacccatttg actgggcaga aaaaaatctc
    33721 caggaagtcg aacgcaataa acgagaaaaa catactgttc cctggcgtta tgtcattctg
    33781 cgcctgcatg aagccgtaca ggaaattgtt ccacatctga atgaacacga ccataaacgg
    33841 ttcagtaaag gccttgcccg ggttttcatc gataattatg cggcaatccc ttcagagtct
    33901 attcgtcggc tgctggcctt acgtgaagcg gggatcattc atattctcgc cctcggtgaa
    33961 gactacaaaa tggaaattaa tgagtcgcgc accgtcctga aaacggaaga caacagctac
    34021 tcgtttgacg tttttattga tgcccgcgga cagcgtccgc ttaaagtgaa agatattcct
    34081 ttccctgggc tacgcgagca attacagaaa acaggggatg aaatccctga tgttggcgaa
    34141 gattatacgt tacagcaacc cgaagatatt cgtggacgcg tagcgttcgg cgcgttgccc
    34201 tggttgatgc acgaccagcc tttcgttcag ggacttacgg catgtgcaga aattggtgag
    34261 gcgatggctc gggcggtcgt aaagcctgca tcccgtgctc gtcggcgtct ttcgtttgat
    34321 taaaggtaaa gctatcagca ctcaacaaac tgatgctgtt cattaagacg atagcggact
    34381 ccggttcgaa tattgttttc accttcaatg gcgacggcga agcgaactct ttcgccgtca
    34441 tgatatgcca gtgcggcgct tccccctggc ccgagaataa ttgagtcgac cacgcccgtg
    34501 ctcactaccg ttgaattttc accgctggca atgatgtgta cattgtcgcc gctgttggca
    34561 attctggcgt ttgcgccaaa gctggcgatt tgcaccaggt caccattact ggcgacatga
    34621 caccgttcac ccaatgtaca aacgcgtact cgcattccgg tattcgcaat ccgactacta
    34681 tctccagcgc tgcttatccg cgaagaatta ccggttacag caacccgtgc gcgttcaccg
    34741 gagctgccaa tgtgactgtt aaagccgaca cttccaatcc gcacgctata acccatactg
    34801 gcaatttgcg cggcataccc agcggttacg attcgggcgt tatcctcaca acagccgatg
    34861 cgagcatgat cgtcgctgcg ggcaaactga ctgcaaaatg gtatctctcc ggggtctgtg
    34921 gccagttttt gcatagagga aacttcctga tgcgcgaagt tttcatccgc cagccatttt
    34981 ttccacgcat actcaaccag actctccgcc cagtccgtat atccttcctc aatcagtgca
    35041 tgatgaatat cggcataact accgccgcgt gggaaatggc gcaggaacca gcgatacatc
    35101 accgctccga tacgccattc tcgcaggtct gctcgtgtaa tgatcattcg ttagtgccgg
    35161 tgatagccat cgaccatgct tttaaaggtt tcgtgtggcg tacgccccgt ggtgcaaaga
    35221 taaagatgtc cgaagataaa aaagaggctt ataaatgcca gagcaaaatg cgcctgcaat
    35281 aaccagtatc ttacgccagg aaacacatct cccacggctt gcggatagag acacaacagc
    35341 cccgtcaata gtaacaacgg cagcaatcca cacatgacac caacataggc gacctgctgt
    35401 aagggattaa atttagactg tgttgttgcc gggaaaggat gttcttcccc ctgcataatg
    35461 ccaaacaaat aaaatcgtgt ttgttttgcc gctcgttcca gccacccctg acgacgaatg
    35521 cgatagtggt gaccattatc cccaacggca ttgatcagca caaagccgag ccagcatgcc
    35581 agtaacaaaa atccgcaaac ttcatgcacc gcaaccagac ttttaaccgc agttgcgccc
    35641 accatcgcaa agtgatttat cagcccactt gccagcaaca atacaaagag taacgcattc
    35701 gaccagtgcc ataatctgac ctctttgctg taaagataaa ccttctcacc atgatcggtg
    35761 gctgatttct tcacgccacg agcacgaagc aaggcgtgca gccccaacac caaccacatc
    35821 ccaaccagca atactccggc aatgatcaac cacaccggcc aaaattcggg agtgaactgt
    35881 ggcacatagt tcgctaactg gctctgaaac tgttcagcat gttgcgacgg gttcatacat
    35941 tttccttttt aatcaaatgt tgaccgaacc ggcgatacag atgcggtttc ccggcaccaa
    36001 gtagctgata ttgatagtat ttattttcct gcaaccacgc ctgaatttct gggctatctt
    36061 cgcgaccaaa gatcaatgcg tgttccggac aggcgctgac gcaaatgggc ggaaagcctt
    36121 ttgccagtcg ggactcagcg cagaaatcac atttatctgc cactttagtg acagggttaa
    36181 ggtagcgaac ctggtacgga caagcgccaa tgcaataact gcagccgatg cactgcgatt
    36241 tttctacccg cacgatcccc tgctcatcac gccacgacgc ccctgtcggg catacgtcga
    36301 tgcacggagc atcttcgcag tgctgacatg actggcgaaa aaagtgatac tgcgtttcgt
    36361 tgtcgttatc ggtgactgga atgtgtgcta tcgataaacg gcttccctga gcggggacgt
    36421 ggttagtttt acggcatgcg cgggcgcaaa tattacagcc attgcaccgt gactcgtcat
    36481 gaatcatggc ataccgaact tccttttctt gcctcgtgtt cgctaacaga gaagacgcag
    36541 aaccagtaaa aaagattact gttcccatcc ccagaacaaa tttgcgtcga gtgaaggaca
    36601 tctgattatc ccctaatgca gtggctgcaa ctgtccaccc tatccatgac atctccctga
    36661 aatttcaggg gttctgaaac tgctctacct cgaaccattc gcataagcca taagccttgc
    36721 cataggtcat aaatcgctcg ctaaacaagt acaacaccgc cgtcgatgac tccacctgcc
    36781 gtatatcggc atattcaggt gcaacatcca tcgcggcgat cgcggcttca atctgcgctt
    36841 cctggaagta atatggcgct tgcatcagca tggcgacttt gtaaggacga ggatacgtct
    36901 ggcactcaaa acgaacggcg tgtgcaatgg cgcgacaaat gtcctgctcc acaacttgca
    36961 aagacatcgc cgcgtagttt tcactcatgg cctgcgttga gtaatagtaa tcatcctgac
    37021 ttcccgtcat acaagcgata tccgcgtagt ctccgttctg cttaatgctt tccagcaaaa
    37081 ctgacagttc ctcttctgca accgaatagg gtggcgagag aaacacggtg cgcgccacca
    37141 gctgcccagc cgcagaatgc tggcgaatga actcagccag gatttcagca ggttccggtt
    37201 cggttaccgt atcaacgggg tgacgcatcc cctgcaacca ttgccgcttc gcctcgtcaa
    37261 cttcgctgac tttcgctaac ggcaattcat cctgatggtt catcttaccc attccttacg
    37321 caggcagtag attgtgtgct gccagatcgg cggcaatatc ttccagcccc agacgctgca
    37381 atgtttcgcg ggttggacaa ccaagctgtg ggtcccagcc catttctttg tagaacatcg
    37441 tgagcgaagc atgcatatcg tcacgatcca ttttgtcagt accttcagta aagaccggga
    37501 tctgcggatc cttgtcgaaa acccaggaac agataagatc gtgttcatta cgcatatctt
    37561 tggtttgcat cagttttacc gtgtaggcac gatgcaacgt aaagatacgc tctgcagcta
    37621 aatctaattt ttcctgagtc atctcttcgc cggtgatcgc tttgaagaat ttggcttcaa
    37681 gcgccagatc gccccggtaa ttacggcttt tcagcggcga aacggtcatt ggccagaccc
    37741 agttgcacag ggtgacggcg ttatgcaaac agacccgcaa cagcgaccac tttgcatatt
    37801 taatttttgc gtcattgatt ggcgtgtagt ttttggtttc atcgtaagca tcttcagaac
    37861 caaaaagttc tttcgccact tcacgttgca gtttcaatgg caagccggaa ccaataaagt
    37921 tgatatgggt atgcgtcatg cagtcacggt tgaacataca gttaacaatg gaaccgacct
    37981 gcgctgacgc ttcattggca tggtgaaccg gatagccaaa cggcgaccag agtttatttt
    38041 tcgcgtagcc ccagtactct tcacccaaat tccagcgttc tgcgatggca tatgaaccat
    38101 cagccaggtg actcagctca cccacacgat gcgccagacg gtagtaaaaa tctttaatga
    38161 agttaacgtc acccgcttcc agttgatccc agcgaatttc cgcatactct tcagcaggca
    38221 gaacacgctt gaacacacct ttgctgtaac aataggtaaa gtcgcgatgc aactgcccgt
    38281 agttacacca taggccgtag tcatcgaaca gattcagacc caccaggtta ccaatcacac
    38341 gaccatcgtc tttatcttca aaatctttcg gaccgttcgg gaagatggtg gtatggacaa
    38401 agttggctac acaagtgtta ccacctgtgc tgggcacgcc aaactctttc acccgaggaa
    38461 tattcatttg ggtcatacaa cggatcgggc aagagtggca gccactcatt tttactgtgt
    38521 atttctctgc cgccggacct aagtcaaaaa cggatttata ggtacgaaag ccgaccgtgt
    38581 tctgattgcc tggcggaatt tcccccgttt caatcggacc accttcagcc gcgccccaaa
    38641 acagcccttt acgtgctgtc caacgtgact tgggatctga atactctgcc cacgattgcg
    38701 gcgtacttgg cacgacatgg ttgttattcg caccaataag ttcagtcatc atgtaatcgt
    38761 tgagacgctt catctcctga cgatcggcaa tgttgacccc tttcgtccct tcaaccgcaa
    38821 tcgctttcag gtttttcgaa cccattattg cgccagttcc cgcaccgccg ctgtggttac
    38881 ggctattcaa catgccagag agaggaacaa ggttttcccc agcctgacca atagccgcca
    38941 cacaggtttc cggactggtc aatcgacaaa tttcttccgt cgttgcgcgc gtcccttttc
    39001 cccataagaa atcggctttt tccaggctaa ctttgtcatc tttaatcttc agccataccg
    39061 gtgatttcgc cttcccttcg ataataatga cgtcgtatcc agcgaatttc atttgcgctg
    39121 caaaaaagcc acccatatgg gcatcgacga ctaaatttcc tttggtaaaa gtagaaagtg
    39181 aggtgatatt tacgcgagaa ctacaggggg caccagatcc agttaatggg ccggtagcaa
    39241 agactaattt attcgcttca tcgaaaggtt tcgtgcctgg cggtacttcg tcatacataa
    39301 ttttgtagcc gaagcccatg ccaccgacaa aacttttaaa cttactggaa tcttcgaggg
    39361 taatatttcc tgtcgtgaga ttgactctta atatattacc tgtccaaccg ttagccatga
    39421 ttttttcctt tgcaagattc acacagtaat atctttccac tcgataattt ttaacgcccc
    39481 tgttgggcag gcgtttgcgc attcaccgca taacacacat ttcgaggatt ttttactttc
    39541 ggtatttacg gtggccatca tccacgggca tgccgtagta caggcgctac agccaataca
    39601 gcgtttatga tcgacggtaa tacagccttc tttctgctgc caggtaatcg caccaatcgg
    39661 gcagacgttc atgcattgcg gctctttgca ttgacggcag gtgtccgcgg tatagttgag
    39721 atcgccatac aggccgccgc cagagccaac cccgttgtcg ccaaagaaat aattgcgatg
    39781 gattttgata cgggagaaga atgtccctac tgagccatcg ttgaagttgg tacaagagat
    39841 ttcacagcgg tggcaacccg tgcatcgcgc tcgctgggtc accagcaccc ctttgggtgt
    39901 atttatcaac cctactgtgc cgctatcaat atcttcctgt ttgcaaccca aaagtgacag
    39961 taacgcaggg gcaattgtta gaccagcaag gccttttcct gatatacgca agaattctaa
    40021 tcgcgttaag ccaatatcta atagtggacg atcaaccggg ttcatttatt tcgcatcctc
    40081 ttcgcaaaca cagtgctgac aattcagcag cctgatatta agcattcaac taatacgctg
    40141 ctgactctgt ccccaacacc agatattttc cctaacccga tagtgtatga gaaaagattt
    40201 aacgccgata aaccaatcct ttatggttag gagaaaaaat aatgtgatta ttctgagccc
    40261 ttaacattga tcgttatcaa ttaaaattac aaagcaagaa attataagtg aactgatatt
    40321 tattatcaat tgaaataaat ttaacttaag cgacaattat ttgtgacgtt cattgcagaa
    40381 aataaaaata atattatcgt tatagattaa aatacatacc gcaattcgta taatttcagc
    40441 agttgttatt tagaatgatt aactggaccg caactgaagc aagaatatca ggatgaagct
    40501 taccgcctca tcctgaatgg tcattttacc agtgcgttaa tgacggtttc catcgcctgc
    40561 ttttccagct cactgcgctg aatgcgttta ttggctaatg ctgtacgtaa tacgcctgcg
    40621 ataacaatat gctttggttc ctgccccaga tcgcgcattt ccagcacaac tttaccgaca
    40681 actctgcaca tttctcggta cagttcatcc tcttttgtcc gatttcccat tctttatacc
    40741 tatttatcat gccaactatc agcatatatc aatctaacca attaaacaaa aatcaaacaa
    40801 aatcagacaa ataacgcgat aaattatttt aactgtagca attgagcgat gatatattta
    40861 tacaccggat gaactttcac ttatcctcac actgacaact tcggcaccag acgttgcgca
    40921 aacagcgaag tttttgcgta accttttccc tggaacgtta aatctttgat aacaatttat
    40981 tgtctaacaa gttgtatatt ttttgaaacg ctgtttttgt tttccttttg gattaatttc
    41041 agcgtataat gcgcgccaat tgactcttga atggtttcag cactttggac tgtagaactc
    41101 aacgactcaa aaacaggcac tcacgttggg ctgagacaca agcacacatt cctctgcacg
    41161 ctttttcgat gtcacctatc cttagagcaa ggcaccacca ctttcgtaat accggattcg
    41221 ctttccggca gtgcgcccag aaagcaagtt tctcccatcc ttctcaactt aaagactaag
    41281 actgtcatga aaaagaccaa aattgtttgc accatcggac cgaaaaccga atctgaagag
    41341 atgttagcta aaatgctgga cgctggcatg aacgttatgc gtctgaactt ctctcatggt
    41401 gactatgcag aacacggtca gcgcattcag aatctgcgca acgtgatgag caaaactggt
    41461 aaaaccgccg ctatcctgct tgataccaaa ggtccggaaa tccgcaccat gaaactggaa
    41521 ggcggtaacg acgtttctct gaaagcgggt cagaccttta ctttcaccac tgataaatct
    41581 gttatcggca acagcgaaat ggttgcggta acgtatgaag gtttcactac tgacctgtct
    41641 gttggcaaca ccgtactggt tgacgatggt ctgatcggta tggaagttac tgccattgaa
    41701 ggtaacaaag ttatctgtaa agtgctgaac aacggcgacc tgggcgaaaa caaaggtgtg
    41761 aacctgcctg gcgtttccat tgccctgcca gcactggctg aaaaagacaa acaggacctg
    41821 atctttggtt gcgaacaagg cgtagacttt gttgctgctt cctttattcg taagcgttct
    41881 gacgttatcg aaatccgtga gcacctgaaa gcgcacggcg gcgaaaacat ccacatcatc
    41941 tccaaaatcg aaaaccagga aggcctcaac aacttcgacg aaatcctcga agcctctgac
    42001 ggcatcatgg ttgcgcgtgg cgacctgggt gtagaaattc cggtagaaga agttatcttc
    42061 gcccagaaga tgatgatcga aaaatgtatc cgtgcacgta aagtcgttat cactgcgacc
    42121 cagatgctgg attccatgat caaaaaccca cgcccgactc gcgcagaagc cggtgacgtt
    42181 gcaaacgcca tcctcgacgg tactgacgca gtgatgctgt ctggtgaatc cgcaaaaggt
    42241 aaatacccgc tggaagcggt ttctatcatg gcgaccatct gcgaacgtac cgaccgcgtg
    42301 atgaacagcc gtctcgagtt caacaatgac aaccgtaaac tgcgcattac cgaagcggta
    42361 tgccgtggtg ccgttgaaac tgctgaaaaa ctggatgctc cgctgatcgt ggttgctacc
    42421 cagggcggta aatctgctcg cgcagtacgt aaatacttcc cggatgccac catactggca
    42481 ctgaccacca acgaaaaaac ggctcatcag ttggtactga gcaaaggcgt tgtgccgcag
    42541 ctggttaaag agatcacttc tactgatgat ttctaccgtc tgggtaaaga actggctctg
    42601 cagagcggtc tggcacacaa aggtgacgtc gtagttatgg tttctggtgc actggtaccg
    42661 agcggcacta ctaacaccgc atctgttcac gtcctgtaat attgcttttg tgaattaatt
    42721 tgtatatcga agcgccctga tgggcgcttt tttatttaat cgataaccag aagcaataaa
    42781 aaatcaaatc ggatttcact atataatctc actttatcta agatgaatcc gatggaagca
    42841 tcctgttttc tctcagtttt tttatctaaa acccagcgtt cgatgcttct ttgagcgaac
    42901 gatcaaaaat aagtgccttc ccatcaaaaa aatattctca acataaaaaa ctttgtgtaa
    42961 tacttgtaac gctacatgga gattaactca atctagaggg tattaataat gaaagctact
    43021 aaactggtac tgggcgcggt aatcctgggt tctactctgc tggcaggttg ctccagcaac
    43081 gctaaaatcg atcagctgtc ttctgacgtt cagactctga acgctaaagt tgaccagctg
    43141 agcaacgacg tgaacgcaat gcgttccgac gttcaggctg ctaaagatga cgcagctcgt
    43201 gctaaccagc gtctggacaa catggctact aaataccgca agtaatagta cctgtgaagt
    43261 gaaaaatggc gcacattgtg cgccattttt ttgtctgcta tttaccacta ctgcgtcgcg
    43321 cgtaacatat tcccttgctc tggttcacca ttctgcgctg actctactga aggcgcattg
    43381 ctggctgcgg gagttgctcc actgctcacc gaaaccggat accctgcccg acgatacaac
    43441 gctttatcga ctaacttctg atctacagcc ttattgtctt taaattgcgt aaagcctgct
    43501 ggcagtgtgt atggcattgt ctgaacgttt tgctgttctt ctgctgatag tggtcgatgt
    43561 acttcaacat aacgcatccc gttaggttcc acggaatatt tcaccggttc gttgatcact
    43621 ttcaccggcg ttcccgtccg cacgctggag aacaaggctt taatatccgg tgcattcatg
    43681 cgaatacacc ctgaactgac gcgcaaacca acgctgtccg gcgcactggt gccatgaatg
    43741 aggtattcgc cattaccatg cgcgaggcgc agtgcgtaac gtcctagcgg gttatttggt
    43801 ccggcaggaa cgactggcgg taatttaatg ccacgctcca gcgaacgctg acgaatgcct
    43861 gccgtaggcg tccaggttgg gttagggatt ttctgcccaa cacgcgtttc catcaccggc
    43921 gtttccagcc cctgcaatcc aatacctatt ggataaacct gcacaatatt ttctcccggc
    43981 ggataataat aaaggcgcag ctctgcaagg ttaacgataa tcccctgacg cggtgcatca
    44041 ggtaataaca gttgtgaagg aatagttatc gtcgtaccag gttttggcac cgggacgata
    44101 gtgttattgg cttcaaggat caacattgcc gcagtatcaa aacgtcgggc aatagcctga
    44161 aggtttttat ccccttcttg caccgtatac gtttgatttt gcccaaccag tcggcttccg
    44221 gttggtggta gcggataatc aaccgcccag gcagcctgga tggcgctaaa agcgccgata
    44281 agcgtgagtg taagcaaaga cgcgcgtttc attgtaaacc tcctgtattt gccggagact
    44341 cacgctgaaa cgtcggatgg cgcttatgtt cacctgaaac caaaacactc ctgtgcaggt
    44401 cagtgtaaac attgaccatc cggcaatgtg agccaaccgg atgaaagctg tccctttagt
    44461 ttagctaagt gcagcggctt tggcgcgaat tgcgcgaatc atcgcttcca gaccttgtga
    44521 acgagatggg gtgagatgtt gggtgagtgc cattttttca aaccacggac gcacatcgaa
    44581 actgacaata tcctgcggcg tcatctgatc gtagagaata aagactaccg caataagccc
    44641 tttcacaatc gccgcatcgc tgtcgccctg caattcaata attccgtgcg cattctggcg
    44701 catgacaatc cacacctgac tctggcagcc ctgaatgcta ttttgtgggc ttcggtcttc
    44761 gtcgcgtaat tcagtcagac gctggcccag ctcaataatg tagagatatt tctcttccca
    44821 gttggcgcag cgtaaaaaat tacgcagcaa tttttcttta tccggcaata aagccatagt
    44881 gcctccctgt tatcccagca aacggtgaat acgttgcagg ccggtcacca gacgatccac
    44941 ttcttcatgg gtgttataca tggccagcga cgcccgacac atcgcaggga cgttgtaata
    45001 ggccattaat ggcatagcgc agtgatgtcc ggtacgcaca gcaatgccgt aattatcgag
    45061 aaaactgcca acatcatagg cgtggtgttt accgagatta aaagcaataa cgccaagcct
    45121 gttttgtggg ccatagagag tgagatccgg tacagattcc agctgtgata gcgcataatg
    45181 catcagattc tgttcatact cggctatgtt attaagcccc agcgccgaaa catactccag
    45241 cgccgcgcca agaccaataa tgcccccggt attgggtgta ccggcttcaa accgccatgg
    45301 tgctttggtc caggtagtgc cttcactcag gctgacggtg gcgatcatag aaccgccccc
    45361 ttcccacggc ggcatctcct gcaacaaggc ttctttcaca taaagaatgc caattccggt
    45421 ggggccatac agtttatgcc cggagaacac gtaaaagtcg caatccagcg cctgaacatc
    45481 caccggatga tgcatcaccg cctgagcgcc atccaccagc acttttgcgc catgctggtg
    45541 cgcaagcgtg attatttccg ccagcggatt ttctgttccc agcacgttgg agacatgagt
    45601 aattgccagc aggcgagttt tctcatcaaa cagcgtaggc agcgtctcca gttgcagcgt
    45661 accgtcgggg ttgagaggga tcacacgcag ctctgcgcca acgcgtgcgc aaagcatctg
    45721 ccagggaaca atgttagcgt ggtgctccat ctgactgatg atgatgttat cgcccgcccg
    45781 cacgttgctg ttgccccaac tattggcgac cagattgatc ccttccgtcg tgccgcggac
    45841 gaacaccagc tcttccgccg aacgggcatt aataaacagc gatgcccgct tgcgcacgtt
    45901 ctccattttc tcggtcgcct gggcgcttaa ggtatgaata ccgcgatgca ccgccgcgta
    45961 gccgtgacga taaaactcag cctcggcgtc aatcacctgg cttggtttct gcgcactggc
    46021 ggcgctgtcg agataagcca gcggcaaacc gttcacctca cgagaaagca ccggaaagtc
    46081 ggcccgcact ttgtcgacgg aaaaagtcat cttgcacctc ctggcagccg ttgaccgatt
    46141 cgggccagca cctgctgttt aagcccctca tcacgcagtg cttccgtcag ttcggcagca
    46201 aaggcgtaaa tgatcatctg ctgggcatcc tgctgattga tcccgcgcga gcgcagatag
    46261 aacatctgtt catcatcaat acgccccacc gtcgcgccgt ggctgcattt cacatcatct
    46321 gcatagattt ccagctgcgg ttttgtatcc acttccgcca ttttgcccat cagcagatta
    46381 ttgttggtca tctgaccatc cgttttgatg gcgtgctgcg cgacgttgat caaaccgtta
    46441 aataccgcgc ggcctttgtc gctgacgata gttttgtgca actgtcggct gttacaaaaa
    46501 cctttattgt gttccagcca ggtacgggta tcacacacct cgtttttcac cggcatcgcc
    46561 aggctattga tccgcagcgt gctgttttcg ccattgagtt gcgtactggt gttgtgtcgt
    46621 aacactgcgc cacccagcag gaaactatgg ctaaatgcgg tggcatcctc agccagcaac
    46681 aaatcgttat gagcaaagtg gtgactgagc gggttttcaa acgccagctt gatatgctgc
    46741 aagtgggcgt tcgctgcgac attgatagtg aaccgtgccc cggtaaaatg acgagcatca
    46801 ttaaggctga caaaatgttc gatcaccgtt gcttcggcac cttccgccag atccagatga
    46861 tgccggtaat gggcagtgtt cacctcttca cctgccacgc cctgggtgat atgcattaac
    46921 agcaatggct ttgccggccg ttgaccgcgc ttcacggcga tatgcgtcac gctttgtgcc
    46981 aggctttccg tcaaatgcag aaacacttcc gcctgaatag cgtcgggtaa cccctgacgg
    47041 tcgtcgttaa tgctcacttc atatccgctg ccttcaattg catcgctcag cgcgggcacg
    47101 taacgtccat cgacaaacac cagccgcacg gcgtctaacg ttaacgctaa ggcatcacgc
    47161 tgctgtgggg atatatctcc cgcaatgctg acaaactgac tattggtcag cccttccagc
    47221 ggcgtatatt tccagttttc atgtttacgt gtcggcagtc cggtacgcag caattgttgt
    47281 aaatgctgct gtgcttgcgg tgagcgtttt gtcccttcag cttcaaacaa gtgatgccac
    47341 tgttgcagcg cattactgct gttcggtaag ccagccataa ccctgctcct ccagttgttt
    47401 gaccaacgtg aaatcgccgg atttcacaat tcgtccctga tatagcacat ggacgtaatc
    47461 aggcttgatg tagtcgagaa tgcgttggta gtgcgtaaca atgatgaatg agcgcttgcc
    47521 gtcacgcagc gagttcacgc catcggcaac cacttttaat gcgtcaatat ccagcccgga
    47581 gtccgattca tcaagaatgc ataactccgg ttccagcacc gccatttgca aaatatcgtt
    47641 gcgctttttc tcgccgccgg aaaaaccaac gtttaccgaa cgggttaata aatcttccgg
    47701 catcttcagg agagcgattt tctcttccat caaatcctga aaatcaaagc ggtcgagcgt
    47761 ttcctggccg cgatagctgc gcaccgcatt aagtgccgtt tgcaggaaaa actggttact
    47821 gacgccggga atctccaccg gatactggaa ggccataaag atgccttcgc ctgcacgatc
    47881 ttccggcgac agcgcaagca aatctttgcc tttgaactca accgtgccgc ccgtcacttc
    47941 ataatcttct cgcccggcaa gcgttgccga taaggtactt ttgcccgaac cgtttggccc
    48001 cataatggcg tgaacttcgc cgggacgaac gtcgaggctt aatccacgca ggatagcttt
    48061 atcttccacg ctgacgtgta aatctttaat acttaacatg tttattcctt atccgacgct
    48121 gtgttcaaga ctgatggcga ggagtttttg tgcttcaacg gcaaattcca gcggcagttc
    48181 tgagaacacg tctttgcaga aaccgttaac aatcatcgag atggcgtctt cttcgctaat
    48241 cccgcgttgc aggcagtaaa acagttgatc ttcaccaata cgtgatgtcg ttgcctcgtg
    48301 ctccagttgt gcgctgttat tgcgacattc gacatacggg aaggtatgcg ccccacaatt
    48361 agcgccaatc agcattgagt cgcactgagt gaaattgcgc gcattggttg ccgtcggcat
    48421 gattttcact aagccgcgat aactgttctg actatgtccg gcagagatcc ctttcgagat
    48481 aatggtcgat ttggtgtttt taccgatgtg gatcatcttg gtgccggtat ccgcttgctg
    48541 atgaccgctg gtcagcgcca ctgagtaaaa ctcaccaatg gagttatcgc cgcgcaaaat
    48601 gcagctggga tatttccacg taatcgctga cccggtttct gattgcgtcc atgacatttt
    48661 gctgttttcg ccttcgcaca aagcacgctt ggtgacgaaa ttgagaatac cgccggtgtt
    48721 gttatcgcca gggaaccagt tttgtaccgt ggaatatttc acctcggcgt ttttatggat
    48781 gatgacttcc accaccgccg cgtgtaactg atagctgtca cgcaccggag cggaacagcc
    48841 ttcaatgtag ctgacgtagc tgtcttcgtc ggccaccaga atggtgcgct caaactgccc
    48901 ggttttttcc gcgttaatgc gaaaataggt ggaaagttcc atcgggcagc gcacgccttt
    48961 aggcacataa ataaacgtac cgtcagaggc taccgccgca ttaagcgcgg caaagaagtt
    49021 gtcatttccc ggcaccacag tgccgagata tttacgcacc agttccggat gatcgtggat
    49081 cgcctcacca aaggaacaga aaataattcc ctgctccgcc agtttttcgc gataagtagt
    49141 ggcaaccgaa acagagtcga aaatggcatc caccgccacc tctttgcctt cccgcacggg
    49201 aacgcccaac tgctcaaacg ccgcctccac ctctttactt aaaaaggcgt tcgcgccagt
    49261 ttgctgcacc gcgccaggtt cagacgcgca agtgtcgtca caattaccgc acgatggtgc
    49321 tgagtagtaa ctgtaatcct gataattcag cttgtcgtag tgcgctttca accagtgcgg
    49381 ttcttccatc tccagccatg cgcgataggc gtttagacga aactccagca tccactccgg
    49441 ctcattacgc ttcgccgaaa ttgcgcgcac cacctcttcg tttatcccct ttgccagctc
    49501 atcggtggct aactgggtga agaatccttc tttataattc agcgggccgc cggtccaggt
    49561 tttgacatcg tcagttgctt cagtattacg agacatagta ccgcctatac cccaaagctt
    49621 tcgccacagc cacattcatt ctgggcttta gggttgtgaa atttgaatat ctgattaagt
    49681 ccttcacgaa cgaaatcgac ttccgtgcca tcaataaacg gcatcgcttg cagcgggaca
    49741 aacagcttcg cgccgtcgtg ttcaaacagc agatcgtctt tgtccggctc gctaacactg
    49801 tcgagcacat agccaaagcc cgcgcagccc gtttgcttca cgcctaagcg cacgccgacc
    49861 atacccggct gctttgccac cagctcacgg atgtgtatcg ccgctgcggg tgtcagcgtt
    49921 aagccttgcc aggcgaaatc ttgtgggtta aaggttcctg aatgcatgtc catcgattta
    49981 cctcacttca tcgctttcag cgtataacag catgctagtg ataatgatta tcagttcaac
    50041 ccagcaaacg caggggcttt accgtaaaac atgctttttg cctgctttta ataagcatag
    50101 accctgatgt gtgggttaac aggcacgcta agaattaggt atctcattgt tagataatgg
    50161 ttattatcta aggagcatta aggctgtgat gaaaaagaaa aagttgtatt gaaaatgact
    50221 atttaagaga taggtaaaaa agtgcagcgt tcagaaataa gaaaaccctt aagtctgtgc
    50281 gacacaggct taagggtttc taccccatcc ggcgcttatc tccggcactc tcagtggctt
    50341 agctcttgaa ggggcgataa gaataatctc ataaagctaa cccgccgttt taacacaaac
    50401 tgcgattagt attatttttg aacaatatca ggcggtagat aagcagtatt aagaaggtca
    50461 tcgaacctgg acggaggtta atccaggtcg atttggcgaa cttgcggcat taagtcagga
    50521 tcaatgcacg cccagacgcc aggcaaagta gatttcttct tttaattcag tagaagagag
    50581 agtaagcagg tcagcaaatt caagttctag ttgtttcaga cgtttgagat attgggcagg
    50641 tgaaagattg ctctggtcac ggcgtaaaaa ttcaatggcc agctgggtgg gatcaagttg
    50701 agtagacata gcatcctcgc ttttagacaa gacgtgcaca gtataccacc gtttactgtg
    50761 cagataatga ccaaaagcaa tatgcgtcac acttttctgg tgacaacgtc acaaaatggc
    50821 ggtcgtcaat cgtgacgaac agcacaaacg ccctttctca tcgaagattt caatctgcca
    50881 gacctggtga cgcgaaccga gatgcaacgg tttacatatg ccgcgcaccc gcccttctcg
    50941 tgccgagcgg acgtgattag cattgatttc cagaccaacc actttttgct caccttcggt
    51001 acataaataa ccggcaacgg aaccgatact ttcggccagt accacggatg ctcctccatg
    51061 cagcaacccg aaaggctgct ttgtccgcga gtctactggc attgtcgctt caagggtgtc
    51121 atcaccaata tgttcaaagc gaatatccag gaaccccacc atgtttcctt cacccatagc
    51181 attcagtgct tccagggtga ttttccgttt ccatatcatt taataatctc cagtaaagcc
    51241 tgcacaggat ggcgtacccc cgtgccttca acccgtttta cctggctacg gcaggaatat
    51301 ccggtcgcca gacagcggtt acgcggcagt cgctgcatag cctgatgcca ggataactca
    51361 tagatcccga gcgaattttc atggtttttc gcttcatgtc cgtaagtccc tgccatgccg
    51421 cagcaaccca cgctgacatt ttccagtttc gcgccaaaac gggcaaatat cgcggcccat
    51481 tgtgctggcg cgcccggcaa agcggtaacc tcggtacagt gaccaaagaa ataccatgat
    51541 tcaccgctga ctgtagccac tggatgtgac tcaagtgcgc ttgccagcca ttcattcgca
    51601 agtaagacgt taaactcgcc acgttcctcg cccagcgcca gtttatattc atcgcgataa
    51661 caaagtacca gcgccggatc gacgcccacc attggcatcc caagcttagc catacggttg
    51721 aggaaatccg ccgtcttttt cgccgtcttc gcaaaacgat taagaaagcc tttgatatgc
    51781 tgggctttgc catttggcga aaatggcagt aacacaggct ggaaacctaa tttttcaacc
    51841 agacggacaa aatccgccac cacttgcgca tcgtaatagc tggtaaaggg gtcctgcacc
    51901 accaacaccg tgcgcgcttt ctgctctgca ttgagagctt caagctgttc cagcgtcatg
    51961 tttgccgagc gatgccccac catttgttgt tgtagcgagg ggaccgacag cagtggcaaa
    52021 tcaaccatgc cgatatgttt ttccgagagt ttgcgcacca gcggctggtt aatgaagaag
    52081 ttaaaggttt tcggcgcgcg tgccatcagc ggtgcgtagc tctcgaccgt agcgacgagg
    52141 tggtcgcgca gcgggcgtaa ataacgggtg tgatagagct gcaggaaacg agagcgaaac
    52201 tccggcacat caattttgat ggggcactgg gtcgaacacg ctttacaggc cagacagccc
    52261 gacatcgcct ctttgacttc gtgtgagaag tcatattcgc ctttattcgc atgccagcta
    52321 ttgcgcgtgc gggcaattaa cgtccgtaaa ctgacgccgg attcaggcag ttcttgttcc
    52381 agtttgagtg gatcaacgcc gcgatccgcc aacaaacgca gccattcacg caccagcgtt
    52441 gcgcgccctt tcggtgaatg aatccggttc tgggtgatct tcatcgacgg acacatcgga
    52501 ctacgggcat caaagttgaa gcataaaccg ttgccgttac actccatcgc accgcgccac
    52561 tgctggcgta ccgcaatggg gatctgccga tcgaatgtac cgcgcttcac cgcgtccact
    52621 ttcatcatcg gcgcatcgag accttctggc gggcaaatct tccctgggtt gagtcggtta
    52681 tgcgggtcaa atgccgcttt cactttgcgc agttctgcaa aaagttcctc accgaaaaac
    52741 gccgggctgt attcagcgcg aaaacctttg ccgtgctcgc cccacaacaa accaccgtat
    52801 ttcgcagtca gcgccaccac gtcatcagag atttgcttca tcaaaatctc ttgttgcgga
    52861 tcgcacatat ccagtgccgg acggacgtgc aaaacacctg cgtcgacgtg accgaacata
    52921 ccgtagctta agccgtggct gtcgagcagc gcgcgaaatt cagcaatata atcggccaga
    52981 tgttccggcg gtacgcaggt atcctcagca aacggaattg gcttagcggc acctttggca
    53041 ttgccaagca gaccaacggc ttttttgcgc attgcataga tacgttcaac ccccgccagc
    53101 tcgcggcaca cctgccagcc aatcacacct gcctgttggc tggcaatcag ctcatcaagc
    53161 cgcacacaga gtgcattaac tcgctcgtca atcagcgcct catcatcacc agcaaattcc
    53221 acaatgttca gcccgagcat ctctttgtca ggcacatcgg taatcaactc gctgacggaa
    53281 tgccagacaa tatcttcccg cgccagattc agcacttttg agtccaccgt ctctaccgaa
    53341 agcgcccgcg cctcaaccat aaacggcgcg ttacgcagcg cggagtcaaa agagtcatat
    53401 ttgacgttca ccagacggcg cactttaggc aagggcgtaa tatccagccg cgcttcggta
    53461 ataaaggcca gcgtcccttc tgaacccgtc agaatgcgcg tcaggtcgaa ctcggtcatc
    53521 tcatcgttaa agacatgacg cagatcgtaa ccggtaagaa agcggttaag tttggggaag
    53581 ttgtcgatga ttaactggcg ttgcagacgg caacgttgat aaaccgtgtt ataaattcgc
    53641 ccgattgtgg tattggtttt acccagcgtt tccgccagtt cgacgggtaa aggttgcgta
    53701 tcgagaatat cgccccccaa caacaccgcg cgtacgccaa gtacgtgatc tgacgttttg
    53761 ccatagacca gcgatccctg accggatgca tcggtattga tcatcccgcc gagcgttgcc
    53821 cggttgctgg tcgaaagttc tggtgcaaaa aagtagccga acggtttcag gtactgattg
    53881 agttgatctt ttatcacccc ggcctcaacg cgcacccagc cctcttcagg gttaatttcg
    53941 atgatgcggt tcatatggcg ggacatatca acaataatcc cctggttgag cgcctgaccg
    54001 ttagtgccgg tgccgccgcc gcggggggta aagatcagcg atgaatagcg ttcctgcgcg
    54061 gcaagacggg cgatcagcgc cacatctgcg gttgaacgcg gaaataccac cgcatcgggg
    54121 agaagttggt aaatactgtt gtcggtcgac attgtcagac gatcggcata acttgtcgcc
    54181 gtatcgccgg taaaaccttg ttgctccagc tcttgcaaaa aattaagcac cagttgaacg
    54241 acgccgggtg cctgggaaat ctgtggaatc attatattga ccctttcctg cggtctgtga
    54301 tgtaggtcga tacactattc tttcaggcgg ctgcaatagc gcactgaaag gtgatgttta
    54361 tttactctat ggatttcgag ttgcaggaag gcggcaagcg agtgaactcc aggagcttac
    54421 aatagtaagt gactggggtg aacgaacgta gccgcagcac atgcaacttg aaatacgacg
    54481 agtaaatcgt ttgcgtgttg cctgagttgt tgtaccacat ttttttctaa cacgcccatc
    54541 agaattaagg gcagaatcgg cctgttaaaa accgctgaaa ttgctcatca ttatgcaggt
    54601 gagtttcgcg tgttcacgtc gcgtcgacga tttgacgcac aaaaaaggtg aaaagtagtt
    54661 atggtaaatg ttcgtcagcc cagggatgtc gcacaaattc tgctttcggt gctgttttta
    54721 gccatcatga ttgtggcatg tctgtggatt gttcaaccct ttattctcgg ctttgcatgg
    54781 gccggtacgg tggttatcgc cacctggccg gtattgttac gtttgcaaaa gatcatgttt
    54841 ggccgccgct ctctcgccgt tctggtgatg acgctgttat tagtgatggt gtttatcatc
    54901 cctattgctt tgctggttaa cagtatcgtc gacggcagcg gcccgctaat taaagccatt
    54961 tccagcggcg acatgacgtt acccgatctg gcgtggctta ataccattcc ggtgattggc
    55021 gcgaagctgt atgcaggctg gcacaacttg ctggatatgg ggggtacggc gatcatggcg
    55081 aaagtccgcc cttatattgg caccaccacc acctggttcg ttgggcaggc ggcgcatatc
    55141 gggcgcttta tggtgcattg tgcgctgatg cttctcttca gtgccctgct gtactggcgc
    55201 ggtgaacagg tggcacaagg cattcgccat tttgcaaccc gtctggcagg cgttcgcgga
    55261 gatgccgccg tgctgctggc ggcacaggct atccgcgcgg tggcgctggg tgtggtggtg
    55321 acggcgttag tacaggcagt gcttggcagt atcggcctcg ccgtatccgg cgtaccttat
    55381 gcaacgttgc taacggtgtt aatgatcctc tcctgccttg tccagcttgg cccgttgccg
    55441 gtactgattc cggcgattat ctggctctac tggaccggcg ataccacctg gggaacggta
    55501 ttgttagtgt ggagcggtgt ggttggcacg ctggataacg tcatccgccc aatgttaatt
    55561 cgcatgggtg ccgatttacc gctgatcctg atcctctctg gcgttattgg tggtttgatt
    55621 gctttcggga tgatcggtct gtttattggt ccggttctgt tagccgtttc ctggcgtctg
    55681 tttgccgcgt gggtggaaga agtcccgccg ccgacggacc aaccggaaga aattctcgaa
    55741 gaacttggcg aaatcgagaa accgaataag taatttctca tcaggcggct ctgccgcctg
    55801 attgttaatc accgctaatt gatatttgct tgctcttccc catattttcc tgcttaccgc
    55861 cattcagct
//
LOCUS       DAKISA010000014        55327 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.014, whole genome shotgun sequence.
ACCESSION   DAKISA010000014 DAKISA010000000
VERSION     DAKISA010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55327
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.014"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(33..992)
                     /gene="accA"
                     /locus_tag="OVW45_000904"
     CDS             complement(33..992)
                     /gene="accA"
                     /locus_tag="OVW45_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCV5525418.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAVSLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            complement(1005..4487)
                     /gene="dnaE"
                     /locus_tag="OVW45_000905"
     CDS             complement(1005..4487)
                     /gene="dnaE"
                     /locus_tag="OVW45_000905"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCV5525419.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDIQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            complement(4524..5120)
                     /gene="rnhB"
                     /locus_tag="OVW45_000906"
     CDS             complement(4524..5120)
                     /gene="rnhB"
                     /locus_tag="OVW45_000906"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCV5525420.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHITP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            complement(5117..6265)
                     /gene="lpxB"
                     /locus_tag="OVW45_000907"
     CDS             complement(5117..6265)
                     /gene="lpxB"
                     /locus_tag="OVW45_000907"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCV5525421.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKEHVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            complement(6265..7053)
                     /gene="lpxA"
                     /locus_tag="OVW45_000908"
     CDS             complement(6265..7053)
                     /gene="lpxA"
                     /locus_tag="OVW45_000908"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCV5525422.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            complement(7057..7512)
                     /gene="fabZ"
                     /locus_tag="OVW45_000909"
     CDS             complement(7057..7512)
                     /gene="fabZ"
                     /locus_tag="OVW45_000909"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCV5525423.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            complement(7617..8642)
                     /gene="lpxD"
                     /locus_tag="OVW45_000910"
     CDS             complement(7617..8642)
                     /gene="lpxD"
                     /locus_tag="OVW45_000910"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCV5525424.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            complement(8646..9131)
                     /gene="skp"
                     /locus_tag="OVW45_000911"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             complement(8646..9131)
                     /gene="skp"
                     /locus_tag="OVW45_000911"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCV5525425.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            complement(9253..11685)
                     /gene="bamA"
                     /locus_tag="OVW45_000912"
     CDS             complement(9253..11685)
                     /gene="bamA"
                     /locus_tag="OVW45_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCV5525426.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            complement(11715..13067)
                     /gene="rseP"
                     /locus_tag="OVW45_000913"
     CDS             complement(11715..13067)
                     /gene="rseP"
                     /locus_tag="OVW45_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCV5525427.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            complement(13079..13936)
                     /gene="cdsA"
                     /locus_tag="OVW45_000914"
     CDS             complement(13079..13936)
                     /gene="cdsA"
                     /locus_tag="OVW45_000914"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCV5525428.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            complement(13949..14707)
                     /gene="ispU"
                     /locus_tag="OVW45_000915"
                     /gene_synonym="uppS"
     CDS             complement(13949..14707)
                     /gene="ispU"
                     /locus_tag="OVW45_000915"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCV5525429.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGRIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            complement(14896..16092)
                     /gene="ispC"
                     /locus_tag="OVW45_000916"
     CDS             complement(14896..16092)
                     /gene="ispC"
                     /locus_tag="OVW45_000916"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCV5525430.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            complement(16184..16741)
                     /gene="frr"
                     /locus_tag="OVW45_000917"
     CDS             complement(16184..16741)
                     /gene="frr"
                     /locus_tag="OVW45_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCV5525431.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            complement(17033..17758)
                     /gene="pyrH"
                     /locus_tag="OVW45_000918"
     CDS             complement(17033..17758)
                     /gene="pyrH"
                     /locus_tag="OVW45_000918"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCV5525432.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            complement(17905..18756)
                     /gene="tsf"
                     /locus_tag="OVW45_000919"
     CDS             complement(17905..18756)
                     /gene="tsf"
                     /locus_tag="OVW45_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCV5525433.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            complement(19017..19742)
                     /gene="rpsB"
                     /locus_tag="OVW45_000920"
     CDS             complement(19017..19742)
                     /gene="rpsB"
                     /locus_tag="OVW45_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCV5525434.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            20110..20904
                     /gene="map"
                     /locus_tag="OVW45_000921"
     CDS             20110..20904
                     /gene="map"
                     /locus_tag="OVW45_000921"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCV5525435.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            20966..23638
                     /gene="glnD"
                     /locus_tag="OVW45_000922"
     CDS             20966..23638
                     /gene="glnD"
                     /locus_tag="OVW45_000922"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCV5525436.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLHYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            23669..24493
                     /gene="dapD"
                     /locus_tag="OVW45_000923"
     CDS             23669..24493
                     /gene="dapD"
                     /locus_tag="OVW45_000923"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCV5525437.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            24548..25360
                     /gene="yaeI"
                     /locus_tag="OVW45_000924"
     CDS             24548..25360
                     /gene="yaeI"
                     /locus_tag="OVW45_000924"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HCV5525438.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWELGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            25522..25908
                     /locus_tag="OVW45_000925"
     CDS             25522..25908
                     /locus_tag="OVW45_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCV5525439.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(25997..27154)
                     /gene="cdaR"
                     /locus_tag="OVW45_000926"
     CDS             complement(25997..27154)
                     /gene="cdaR"
                     /locus_tag="OVW45_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HCV5525440.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(27309..28733)
                     /gene="degP"
                     /locus_tag="OVW45_000927"
     CDS             complement(27309..28733)
                     /gene="degP"
                     /locus_tag="OVW45_000927"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCV5525441.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            complement(28863..30380)
                     /gene="dgt"
                     /locus_tag="OVW45_000928"
     CDS             complement(28863..30380)
                     /gene="dgt"
                     /locus_tag="OVW45_000928"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCV5525442.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEQLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            30464..31162
                     /gene="mtnN"
                     /locus_tag="OVW45_000929"
     CDS             30464..31162
                     /gene="mtnN"
                     /locus_tag="OVW45_000929"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCV5525443.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            31155..31955
                     /gene="btuF"
                     /locus_tag="OVW45_000930"
     CDS             31155..31955
                     /gene="btuF"
                     /locus_tag="OVW45_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCV5525444.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            31993..32616
                     /locus_tag="OVW45_000931"
     CDS             31993..32616
                     /locus_tag="OVW45_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCV5525445.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEVGPLIAVCMVVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(32663..33007)
                     /gene="erpA"
                     /locus_tag="OVW45_000932"
     CDS             complement(32663..33007)
                     /gene="erpA"
                     /locus_tag="OVW45_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCV5525446.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(33000..33065)
                     /locus_tag="OVW45_000933"
     CDS             complement(33000..33065)
                     /locus_tag="OVW45_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525447.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            complement(33089..34510)
                     /gene="clcA"
                     /locus_tag="OVW45_000934"
     CDS             complement(33089..34510)
                     /gene="clcA"
                     /locus_tag="OVW45_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCV5525448.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            34735..36015
                     /gene="hemL"
                     /locus_tag="OVW45_000935"
     CDS             34735..36015
                     /gene="hemL"
                     /locus_tag="OVW45_000935"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCV5525449.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIIEPVAGNMNCVPPLPD
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            complement(36173..38155)
                     /gene="fhuB"
                     /locus_tag="OVW45_000936"
     CDS             complement(36173..38155)
                     /gene="fhuB"
                     /locus_tag="OVW45_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCV5525450.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLYRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(38152..39042)
                     /gene="fhuD"
                     /locus_tag="OVW45_000937"
     CDS             complement(38152..39042)
                     /gene="fhuD"
                     /locus_tag="OVW45_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCV5525451.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            complement(39042..39839)
                     /gene="fhuC"
                     /locus_tag="OVW45_000938"
     CDS             complement(39042..39839)
                     /gene="fhuC"
                     /locus_tag="OVW45_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCV5525452.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            complement(39890..42079)
                     /gene="fhuA"
                     /locus_tag="OVW45_000939"
     CDS             complement(39890..42079)
                     /gene="fhuA"
                     /locus_tag="OVW45_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCV5525453.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMRQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            complement(42299..44833)
                     /gene="mrcB"
                     /locus_tag="OVW45_000940"
     CDS             complement(42299..44833)
                     /gene="mrcB"
                     /locus_tag="OVW45_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCV5525454.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDGELYE
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            complement(45029..47458)
                     /gene="hrpB"
                     /locus_tag="OVW45_000941"
     CDS             complement(45029..47458)
                     /gene="hrpB"
                     /locus_tag="OVW45_000941"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCV5525455.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERHYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFEPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLMAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPLMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGGVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            47532..48062
                     /gene="thpR"
                     /locus_tag="OVW45_000942"
     CDS             47532..48062
                     /gene="thpR"
                     /locus_tag="OVW45_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCV5525456.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            48077..48781
                     /gene="sfsA"
                     /locus_tag="OVW45_000943"
     CDS             48077..48781
                     /gene="sfsA"
                     /locus_tag="OVW45_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCV5525457.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATRGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMNVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLSVTL"
     gene            48959..49414
                     /gene="dksA"
                     /locus_tag="OVW45_000944"
     CDS             48959..49414
                     /gene="dksA"
                     /locus_tag="OVW45_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCV5525458.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            49451..50377
                     /gene="gluQRS"
                     /locus_tag="OVW45_000945"
     CDS             49451..50377
                     /gene="gluQRS"
                     /locus_tag="OVW45_000945"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCV5525459.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWTLTAVPESAIVNSTFSNASC"
     gene            50416..51834
                     /gene="pcnB"
                     /locus_tag="OVW45_000946"
     CDS             50416..51834
                     /gene="pcnB"
                     /locus_tag="OVW45_000946"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCV5525460.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEDEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRTWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            51831..52310
                     /gene="folK"
                     /locus_tag="OVW45_000947"
     CDS             51831..52310
                     /gene="folK"
                     /locus_tag="OVW45_000947"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCV5525461.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDELSKW"
     gene            52680..53264
                     /locus_tag="OVW45_000948"
     CDS             52680..53264
                     /locus_tag="OVW45_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCV5525462.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            53369..54109
                     /locus_tag="OVW45_000949"
     CDS             53369..54109
                     /locus_tag="OVW45_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCV5525463.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDNNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            54144..55327
                     /gene="htrE"
                     /locus_tag="OVW45_000950"
                     /pseudo
     CDS             54144..55327
                     /gene="htrE"
                     /locus_tag="OVW45_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
BASE COUNT        13443 a        15143 c        13538 g        13203 t
ORIGIN      
        1 ccgaagtgac cctttttcag aacttttgcg aattacgcgt aaccgtagct catcaggcgc
       61 tgataacgac gattttttaa atcttcagtg cttaacacat cgagatcggc cagatccgcc
      121 agcagttgcg ctttcaacga taccgccatc gcttccgggt tacggtgagc gccgcccagc
      181 ggttccggga tgatggagtc gatcagtttc agctctttca gacgcggagc gatgataccc
      241 atcgcttcag ccgccagcgg cgctttgtcg gcgctcttcc acagaatgga cgcacaacct
      301 tccggcgaga taacggaata ggtgctgtat tgcagcatat tcactttatc gcccacgcca
      361 atcgccagcg caccgccaga accaccttca ccgataaccg tacaaactac cggtacgccg
      421 aggcgagaca tttcacgcag gttgcgtgca atggcttcag actgaccacg ctcttctgcg
      481 cccacgccag gataagcccc cggggtgtcg ataaaggtga tgataggcat cttaaagcgt
      541 tcagccattt gcatcagacg cagtgctttg cggtaacctt ctggcgctgg cataccaaag
      601 ttacggcgaa ttttttcttt ggtttcacga cctttttgat gaccaatgat catcaccgga
      661 cgaccgtcaa gacgggcgat accaccgacg atagctttat cgtctgcata cgcgcggtcg
      721 ccagccagtt cgtcaaattc atcaaatgcc aggcgaacgt aatccagggt ataaggacgc
      781 tgtggatggc gtgccagttg cgcaatctgc catgcaccga gatcggcgaa gattttacgt
      841 gtcagttcta cgcttttttc acgcagacga tgcacttctt catcgatgtt aatatccagt
      901 ttctcatcct gacggctaac cgcagtcaga gaatcgattt tcgcttccag ctctgcaatc
      961 ggctgttcaa aatcaaggaa attcagactc atagtattcc tgtattagtc aaactccagt
     1021 tccacctgct ccgaaccaat gaggccacgg agatcgttta ataaacgatc gctcggagag
     1081 acacgccacg tcgcgccaaa acgcaaccgc gcgcgtgcat ccgccctctg atagtagaga
     1141 tgtactggaa ttgtcccaga gcggtggggt tccagagact gacggagtcg gtttaaaagc
     1201 tggtcatcaa tttgcctgtc cgtcagcgag atagcaagcc cgcgagcata tttttcccgg
     1261 gcttcgtcaa tatccatcac ttcgcgagcg gtcattttaa gcccaccgct gaagtcatca
     1321 aagctgacct gtccgctgac gataagtatg cggtcttttt ccagcaattg ctggtattta
     1381 tccagggcgt cagtaaacaa catcacttcc agccgcccgg aacggtcatc cagcgtgcag
     1441 ataccgatac gattgccgcg cttggtgacc ataacccgcg cggcaacaac gagccccgca
     1501 gccgtgatga ctttaccacg ttctgtcggg tgcatgtctt tcagccttac gcctccgaca
     1561 taacgctcaa tctcttttaa atactggttg atagggtgtc ccgtaaggta cagacctaac
     1621 gtttcacgtt ccccatctaa caccacctgc tccggccacg gttggcagct ggcgtaggat
     1681 tgttcaattt gttccggctc ttcggccagc acgccgaaca tatcggcctg accgatagct
     1741 tccgctttcg cgtgttgatc tgccgctttt aacgcatcac ccagcgagtt catcagcgcc
     1801 gcgcggtgtg gcccaagacg gtcaaacgcc ccggacatga tcagtttttc cagcacccgc
     1861 cggtttaact ttttggtgtc ggtacgggcg cagagatcaa acagttcgcg gaagtagccg
     1921 cctttattac gggcttcgat gatggcctca atcggacctt cacctacccc tttgatggcg
     1981 ccaataccat acacgatttc gccgtcgtcg ttgacgtgga aatggtaaag accggagttt
     2041 atatctggtg gcaggatttt cagccccatc cgccagcact catccaccag gcccaccacc
     2101 ttctcggtgt tgtccatatc ggcggtcatt accgccgcca taaactccgc cggatagtgc
     2161 gctttcagcc ataacgtttg atatgacacc aaagcatagg ccgcagagtg cgatttgtta
     2221 aatccgtaac ccgcgaattt ctccaccagg tcgaagattt tcatcgccag ttcggcgttg
     2281 attccgttct tttctgcacc ttcagcaaat acagaacgct gcttagccat ctcttccggc
     2341 tttttcttac ccatcgcacg acgcagcata tccgcgccac cgagggtata accagaaagc
     2401 acctgcgcaa tctgcatgac ctgttcctga tacaggatga tgccgtaggt tggctccagt
     2461 accggtttca ggctttcatg ctgccactgt atgtccggat aggaaatctc ttcgcgacca
     2521 tgtttacggt cgataaagtt atccaccatc cctgactgca acgggccggg gcggaacagt
     2581 gccaccagtg cgatcatatc ttcgaagcag tcaggttgca gacgcttgat caggtccttc
     2641 atgccgcgcg attcaagctg gaataccgcc gtggtttccg agcgttgcag catgtcgaag
     2701 cttttcttat catccagcgg gatcgcagcg atatccagcg gcggctcgcc attcttcgcc
     2761 cgccgcttgt tgatcatctc cagcgcccag ttgatgatgg tgagcgtacg caaaccaagg
     2821 aagtcgaact tcaccagtcc ggcgtattca acgtcgcttt tatcaaactg ggtgaccgga
     2881 tgtttgccct cttcatcgca gtaaagcggc gcaaaatcgg taattttggt cggcgcgata
     2941 accaccccac cggcgtgctt accggcgtta cgggtgaccc cttccagttt gcgcgccatg
     3001 tcgatcagcg ccttaacttc ttcatccgct tcgtagattt ccggcagctg cggctcggct
     3061 tcaaacgctt tcgccagcgt catccccgga tcgggcggga tcagtttcga gatacgatcg
     3121 acaaagccgt acggatgccc cagcacgcgg cctacgtcgc ggatcaccgc tttcgccgcc
     3181 attgtaccga aggtgatgat ctgcgatacc gcatcacgac cgtacatgtc cgctacgtgc
     3241 tcgataacct gatcgcgttt ctccatacag aagtcaacgt cgaagtcagg catggagaca
     3301 cgttccgggt taaggaaacg ttcgaacagc aggtcaaatt ccagcggatc gaggtcggtg
     3361 attttcagcg cgtaggccac cagtgaaccc gcaccggagc cacggcctgg ccctaccggt
     3421 acgccgttat ctttcgacca ctggataaat tccataacga tgaggaagta gcccgggaag
     3481 cccatctggt tgataacctg aagttcagtc tccagacgtt cgtcatattc cgggcggcgc
     3541 ttaacacgtt cttcctcgtc agggaataaa aaggccagac gctcttccag gccctctttt
     3601 gcacgcttga ccagataatc ttcggtgctc atgtccccgg tcgggaactg cggcaggaag
     3661 tattcaccaa gacgcacggt tacgttacaa cgtttggcga tctcaacggt gttggcaaga
     3721 gcttcgggga tgtcggcaaa cagctcgcac atctcctctt cgctacgcat atattgctgc
     3781 ggcgaatagt tacgcgggcg tttaggatcg tcgagggtaa agccgtcgtg gatcgcgacg
     3841 cggatttcgt gtgcgtcaaa gtcgctgctg tcgataaagc gcacgtcgtt ggtcgccacg
     3901 acgggcaaac cgcgcgcttc cgccagttcc accgccgcgt gcagatagct ttcttcgtcc
     3961 ggcctgccgg tgcggatcag ctcgagaaaa tagcgatccg ggaagtgttc ttcataaaac
     4021 gcgacacact catctaccag cgcgctgtta ccacgcaaaa gactgcgtcc gacgtcgccc
     4081 atgcgtccgc cggaaagaag gatcaaccct tcgtttaatt cgataagcca gtcgcgatcg
     4141 atgatcggcc cggcggcacc gtacccgcgc tgatacgctt ttgagatcag caacgtcaga
     4201 ttctgatagc cggtattgtt cgccgccagt accgtcaggt gggttaactc atcacccagc
     4261 aggtcgcact ggacgttaaa atctgccccg acgataggct taatccctgc gccatgtccc
     4321 gctccgtaga acttcaccag accacaaagg ttggtgaaat cggtgatcgc cagtgctggc
     4381 atacccaacg ccgccgcctt ttttaccaac ggtgcggttt tggccaggcc atcgatcatc
     4441 gagtagtcgc tgtgcacccg caggtgtacg aaacgtggtt cagacatctt cagattccgg
     4501 tttacttaat ctcgacacaa gaatcaggac gcaagtccca gtgcgcgttt gacaggccca
     4561 aagctgcgcc gatggtgttc ggtcgcgccg tgttcagcca gtttttccag atgaaaagcg
     4621 gttgggtacc ctttgtgttg ggcaaaacca tattgcggga aaacaatatc cagcgccgcc
     4681 atttcggcgt cacgcgtcac tttcgccagg atagacgcgg cactgatttc cggtacgcgg
     4741 ctatcgcctt tcaccacagc catcgcaggc atcggtaatt tcgggcagcg gttaccatca
     4801 atcaacacat attccggcgt aatatgcagc ccagcgacgg cacgctgcat cgccagcatg
     4861 gtcgcatgaa gaatgttcag ctcgtcgatt tcgtggggtt ccgcgcggcc cagactccag
     4921 ctcaacgctt tctctttgat ctcttcatag agcgccagac ggcgtttttc gctcagcttt
     4981 ttggaatcat tcagcccggc aatcgggcgc gccgggtcaa ggatcaccgc agcggtgacg
     5041 accgcgccaa ctaacggccc gcgtccgact tcatccacac ccgcaaccag ctgcgtgtgc
     5101 ggataaacaa attcgatcat tgtgctaact ccagaacggc ttgtgccgcc tgctcatcgg
     5161 cattgcagcg gatctgctga tgcagttcac ggaaggtatc gtgcatcgcg tggctggttt
     5221 tcccgttcgc caacagcggt aacagcgccg cagccagttt ttgcggctca cactcttcct
     5281 gcaataattc tttaactaac tctctgcccg ccagcagatt tggcagcgag acataatcag
     5341 ttttcaccag ccgcttcgcc aaccagaagg taaaaggctt catgcgatat cccaccacca
     5401 tcgggcattt cgccagcata cactccaggg ctgccgtacc cgacgccagt agcgccgcat
     5461 cgctggcgac catcgcctca cggcccatcc catccagcaa atgaactgaa aggtctggcg
     5521 cgacttcagc tttgatgcgt tcaaactgct cgcggcgttt ggcattcacc agtggcacca
     5581 cgatttcgag atccggatat gtctggcgca aaagctgggc cgttttcagg aaatcggcac
     5641 taagcatttc aacttctgca ccacggctcc ccggtagcaa cgccaggcag tgggcatcgt
     5701 gagggatccc cagcacatca cgggcggcat ttttatctgg atctaacggc atggcatcag
     5761 ccatggtatg accgataaag cggcacggta cattgtattt gtcataaaac gctttttcga
     5821 aaggcagaaa tgcgagcacc agatcggtgg ctctgcctat tttgaaaacg cgtttctgtc
     5881 gccacgccca gacggatgga ctgacgtaat gaatggtttt gataccctgc tttttgaggt
     5941 taccttcaag agtaatattg aagtcgggcg catcaatacc aacaaaaaca tctggcttca
     6001 gttcgccaaa acgctttgtc agatcggcac gaatatgcag taagcgacgc agacgaccga
     6061 gcacttcaac aatgcccatt accgccagtt cttccatttc gtaccaagct tcgcagcctt
     6121 cagcctgcat tcgtggcccg gcaacaccaa caaagcgggc gttgggcaca tgttctttca
     6181 gagcgcggat taaaccggcc cccaggatat cgccggaggt ttctccggcg accagggcaa
     6241 tcgttaatgg acgctgttca gtcattaacg aatcagaccg cgcgttgagc gtgcaaagaa
     6301 atcggtaaag gctttcactt ccggatatgt ttccgccagt tcagcaattt ccggtttcac
     6361 ttcatcgagc gttttaccgc tacgataaat cagcttatac gcattgcgga tagcggtaat
     6421 cgcctcacgg ctgaatccgc ggcgcttcag cccttcgata ttgacaccga acggcgttgc
     6481 gtggttaccc tgcgcaatga cataaggagg gacgtcctgc gccacaccgg agcagccgcc
     6541 aaccatcacg tgcgcaccaa tgatgcagaa ctgatggact gcggtcatgc cgccgatgat
     6601 cgcgaagtcg tcaaccgata cgtgacccgc cagcgttgcg ttgttggcga gaatacagcg
     6661 gttacctacc gtacaatcgt gcgcaatgtg cgcgttgatc atcagtaagt tgtcgctgcc
     6721 caccttcgtc aatccaccgc cctggactgt gccacgatga atggtgacgc tttcgcgaat
     6781 gcggttacga tcgccgattt ccacacgggt cggttcgcca gcatatttca gatcctggtt
     6841 aacttcgccg atggaggcga actgataaat ctcattatcg cggccaattt tagtatgacc
     6901 attcacgaca acgtgagatt tcagtacggt accctcacca atttcgacat ggggtccaac
     6961 gatacaaaaa ggaccaatgt gtgcgttcgc gccaattgac gcgccctctt ccacaatggc
     7021 ggttggatgc acaaaggcgg atttatcaat cacgtatcag gcctcccggc tacgagcaca
     7081 catcatcgtt gcttcgcaaa ctactttacc atcgaccaga gcaacccctt taaaacgggt
     7141 caggccgcgg cgcgtttttt cgaaagtgac ttccatgatc atttgatcgc caggcacgac
     7201 cgggcgcttg aagcgcgctt cgtcaatacc agcgaagtag tacagctcac ccggttccag
     7261 ttttcctacg cttttaaacg ccagaatacc tgttgcctgt gccattgctt ccagaatcag
     7321 cacacccggg aaaatcggtt ttccagggaa atggccctgg aagaatggct cattgacaga
     7381 gacatttttt actgcgcgca gaaaacgacc ttcttcaaaa tccagcacgc gatccaccag
     7441 taagaacggg aaacggtgcg gcagaagttc taaaatctct tcaatctgca gagtatgagt
     7501 gttagtagtc aagatactct tcctgtcaaa atataagaaa cgacaataat aacacggcct
     7561 gccgcaatcg taagaatgag acaggccgta aagtttggcg aacaaaagat ggaacgttag
     7621 tcttgttgat taaccttgcg ctcaagcgat ttcagacgct tgctcatgtc atcaatgttc
     7681 atcaccagtg cagcggtttt gcgccagact ttgttgggtt gcagcggaat gcctgaggaa
     7741 tagacgcctg gttcagtgat gggacgcatc accataccca tgcccgtaac cgtcactttg
     7801 tcgcatattt ccatatgccc gttgattacg ctggctccgc cgatcatgca gtaacgacca
     7861 attttcaggc tgcccgccat aatgacgcca ccggcaaccg ccgtattgtc gccaatcacg
     7921 acgttatgtg caatctggca ctggttatca atgatcacgc cattgccaat aacagtgtca
     7981 tccagcgcgc cgcgatcgat ggttgtgcag gcaccgatct ccacgcgatc gccaataatt
     8041 acgcgaccaa tctgtgggat cttcacccag ttaccacgat cgttggcata accaaagccg
     8101 tctgcgccta ccactgttcc ggactggatc aggcaattct gaccgatctg gatctcatgg
     8161 taaatggtta cgttcgccca gagacgcgaa cctgcaccga ttttgctgtt tttacctacg
     8221 aagcaaccgg caccgataat cacgttatcg cccagttcaa cgccggactc aatcaccgcg
     8281 ttagcgccaa tcgatacgtt gttacccagc ttcgccgtcg cgtcgatcac cgcactgggt
     8341 gcaatgttct gcgcgggctg cggcgtggta tctaaaattt gcgccatgcg cgcgtaagtc
     8401 aggtagggat tcttcactac cagcgcggca cttttcgcga aaggaagatc gtcctgggtc
     8461 atgacaaccg cggacgcctg gcacaagcct aaatgctcac ggtattttgg gttaaccatg
     8521 aacgtaatgt gacctgtttg tgcagattgc atggacgcaa cgccggtgat gacgatatcg
     8581 ccatcaccgt gtagttctgc atccaactgc tgcgctaaat cagccagtcg aattgaaggc
     8641 attacttatt taacctgttt cagtacgtcg gcagtgatgt cttttacatc gctgctgttg
     8701 taagcaacgg cgtttgcatc aacaaccaga tcgatatcct ggctgttggc aacggatttc
     8761 acagcagtct ggatacgagt aaccagtttg ccgcgttctt cgttggaacg acgtgcgcga
     8821 tcctgctcaa aagcctgcgc tttctgagca aaagtctggc gctgagccat cacgtctttt
     8881 tccagcttag tgcgatcgct gcccgctttc atggactgca gctttttcat tttagcctgc
     8941 agatcggttt ccatacgctg cagttcgctg gcacggcctt tgaactcatt ttccagcgtg
     9001 ttagaaacac cggttttctg cgctacctgc tggaacaggc tgcccatatt gacgattgca
     9061 attttgtcag ccgcctgagc agaagttgcc agtgctaaac cgagacctgc cgctaataac
     9121 cactttttca caataaactc cttaccatcc catttgcacc ggagggtgca gttctttgcg
     9181 tggcccggcg atcttatatt gatcgcctaa agtcatcgct acactaccac tacattcctt
     9241 tgtggagaac acttaccagg ttttaccgat gttaaactgg aactgttctg ccttgtctcc
     9301 atcgtacttt ttgaacggct gggcgtagga gaacaccaac ggccccaatg gggacatcca
     9361 ttgtaatgcg atacccgcag acatacggat attgcttgga tcactatagt ccggatatcc
     9421 agaatattgg ctggaatccc agtttgtatc ccaaacggta cccatatccc agaagaagga
     9481 agtacgaacc gagttagcat acttatcgct aataaacggc gtcggggtga tgaactcgag
     9541 gctggcaacc gccatggcgt taccgcctac agcatcatcc gatttacaca ggtctttcgc
     9601 gccgtcctga gtcgcacatt cgtaatcata gtccggatca taattactgg cctgatgcgg
     9661 gaagtaaact gctttcggac caattgtatt ggactggaag ccacgcacgg tgctggaacc
     9721 accggcatag aagttctcgt agaacggcat ctctttgccg cctaaaccat caccataacc
     9781 ccagcgggta cgccccagaa caacccattt gtgatcgtca tcgatcggca cataagtcgc
     9841 cgtgtctaac gtcactttgt agtattcgtt atccgatcca ggaatggtca ctttaccggt
     9901 caggttgaca cgtgaaccat ctgtcgggaa gtaaccacgg tcaagcttgt tataggtcca
     9961 accatagttg aacgtgaagt cgtccgtttt gaagctgtta tcctgatcag aggtgctcgg
    10021 atgttcaccc atagagtaca gataacgcca catcgcaacc tgaggctgca tgttggacag
    10081 ggagttatgt acataaccca gacctgcacg cagcgagtta tattcgttga tcgggaagcc
    10141 caacgtcacg tctgtaccat aactcttgtt ggtatagtcg gacaggtcgg cgtcatctgc
    10201 ctggaagtca ttatagaaga gacgaccacc gaggcttaca ccatctacag tgaagtacgg
    10261 gttggttacc gacaattcag cataggtctg gtaatcgttt ttggtcccgt tgataccaac
    10321 agcataacct gtacctaacc agttatcctg ttgcacgcca gcctggaagc tcacgccact
    10381 ttcagtaccg taaccaatcc cgaagttgaa gctaccggtg ttacgttctt tcaccttgta
    10441 gactacatct acctggtccg ggctacccgg aacacgttgg gtatcggtat cgacagtttc
    10501 aaagaagccc agacgattca gacgctcctt accctgatcg accagatcgc ttcccagcca
    10561 tgcaccttcc atctgacgca tttcgcgacg caggacggca tctttcgagg tatcgttacc
    10621 ttcaaaacgg atcttacgca cgtagaaacg gttacccgca tcaacgttca cgcgtaattt
    10681 aacggttttg tcggcatcgt taatttcagg catcgactgt acgcgcggat aggcataacc
    10741 atagcgaccg agaagctttt tgatgtcatc ttccatcttg gtcactttgg tgccgttata
    10801 cagctcgccc ggctcgatct tagtcagctg ctcaatttca gcggagtgcc cggcaaggtt
    10861 gccgctcact tcaacgccag aaagcttgta ctgatcgcct tcggtaatgt tcaccgtgac
    10921 gtaaatacct tttttatctg gcgtcagact gacctgggta gagtcgatgt tgaaacgggc
    10981 ataaccgcga tccagatagt agctgcgcag ggtttcaagg tcgcccgcca gtttctgttt
    11041 ctggtattta cgatcgccta ccacgttcca ccacggcact tcgtcacgca gttggaaatg
    11101 agagatcagt tcgtcggtgg tgaaagcatg gttaccaaca atgttaattt gctggatttc
    11161 agctgacaca ccttcctgga acaccagttt taggtcaaca cggttgcgcg gcagcggggt
    11221 cacgacagct tttacgctgg cgctatattt accgacgctg tagtagaagt cttccagacc
    11281 tttctcgata tcggcaatgg tggtgcgatc gagggattcg cccacacgca caccagaagc
    11341 ctcgaggttt tgcttcagca tgtcatcttt caccgatttg ttaccggaga aagtaatgct
    11401 ggcaatggtc ggacgttctt ttacctgaac cagaagggta tcaccatcac gaaggacgcg
    11461 aacatcctca aagttgccgg tagcaaacag agcgcgaatg gtattactga tatcttcatc
    11521 attaaccgtg tcgcctgtgc gcaccggcat actgaggagt gccgcaccaa cggcgacacg
    11581 ctgaaggcct tcgaaatgaa tatctttcac tacgaaccct tcagcaccgt atacggtggc
    11641 gctgctaaac agcagcgacg ctatgagcaa ctttttcatc gccatcgtta ttatgcgttc
    11701 ttcctaacta actctcataa ccgagagaaa tcattgaaaa gtgcaagccc cattaacagc
    11761 accagcagaa tcgagccaat gcgataacaa aagtcttgaa cccgctcgga taccggtccg
    11821 cccttgatct tttcgatcgc aaggaacagc agatgccccc cgtcaagtac gggcaacgga
    11881 aacaggttaa ttatccctaa gttcacgcta ataagcgcaa gaaacggcag gtaataaact
    11941 accccgagtt ccgctgtcat cccagccccc ttggcgatag agatcggccc actgaggttg
    12001 ttcagtttca catcaccggt gatcaatttt cccagcatac tgaccgtcag cttcatcagc
    12061 tgccacgttt tgtccgtggc ttcgacgatg gcgttgaacg gcccatactg gcgtacaact
    12121 ttatactcat ctggcaaagg aatgactttc ggctcaatac cgacaaaacc aatcgcttta
    12181 ccattacccg gtttactctc cgggattaat gtcaaagaca agggactccc ctgcctttcg
    12241 atttctaacg ctaaggattt acccgggtta tcccggacaa gcatcacaaa ggtcacccac
    12301 tgcgttaagg gctgaccatc gactttaacg atcctgtcgc ctgcttgcaa acctgccttg
    12361 cttgccgccg agtttggctg cacattttcc agtacaggtt caatttgcgg cccacgagga
    12421 cgaatcccca gcgaagatac cggatcttct ttatcaggct caaacgccca gtgacgtaaa
    12481 tcgagcttta catcccgccg ttggtcgctg ccaaatggcg ctactgtaat ggtggtgctt
    12541 tcatcgccaa ttttatcgac caactgcaaa cgcacggcat cccaatcagg cgtttcgata
    12601 ccatctacgg cttttagttc cgtacctggt gcaatttgtg cttccgcagc tatcgaattg
    12661 gctgctattt caccaaccac cggacgtacg ccaggcacac caataataaa aaccagccag
    12721 taggcaaaga tagcaaaaat gaagtttgca accggacctg cggcaataat cgccgctcgt
    12781 tggccaacag atttattatt gaaggcatgg tggcggagtt ccggaacgac cggttctgcg
    12841 cgctcatcca gcattttgac ataaccgccc agcggaatca gggcgataac atattcggtg
    12901 ccgagcttat cagttcgccg ccagagcgcc ttaccaaacc ctattgagaa acgctcaacg
    12961 cgaacaccac aacgccgggc aacccagaaa tgaccaaatt catgcacggt gataagtaca
    13021 cccagtgcaa cgatgaacga agccaaatcc cagagaaaac tcagcatatt accttccgtt
    13081 aaagcgtcct gaataccagt aacaacaagc aagcaaagac cggtaccgca gccgtcaggc
    13141 tatcaatacg atctaaaata ccaccgtgtc ctggaattaa atgaccgctg tccttaattc
    13201 ctgcttcacg cttaaacata ctctcggtca gatcgccgag cactgaggct aacgctgcga
    13261 caatagagca aatgagtaag gtgacgggag cgacgtcgag attcgcccac atgccataac
    13321 cccatgagat taccgctgcg gtcgctagtc cacctataaa gccttgccag gttttacccg
    13381 gagaaacctt cggtgccagc ttatgtttac caaacaattt gccaaacata tatgcgccgg
    13441 agtcagcgcc ccataccaag atcatgacat agagcagcca tattgcgcca ctgtaatgat
    13501 tctcgtcata gtgccaggcc cgtaacgcca gcatgcccca gaagaaggga acaatggtta
    13561 gcacgccaaa aataaggcgc aatgttttag agttacgcca gattgctgcg gaacctgggt
    13621 aaaacagcac cagcaatagc gcgacaatcc accaacccaa cgaagcccaa agtgagattt
    13681 caaccagcgg ttgatgaata tttcggtgat attccggcaa cagaaaaagc atcagcgcca
    13741 acaataaccc gcataacacc gccaaccata ctcgctgcga acgagtggta aaaccgctaa
    13801 gctgtcccca ttcccacgct gccagcatgc agaccaccag cgttacgatg gcgaacccca
    13861 ccggcggcaa cagaaacaac gccgcgatga cgacgggtat taacacaaaa gcagatatca
    13921 ggcgatactt cagcaaaagc gccccccatc aggctgtttc atcaccgggc tcggtgccgc
    13981 cgaaacgacg ctctcgatta gcaaaggcat ttaacgcccc ttcaaagtct tgttcatcga
    14041 aatcgggcca gagaacatct gtaaagtaaa gttcggcata ggcaatttgc caaagcaaaa
    14101 agttactaat gcgatgctcc cccccagtcc taattactaa atctacaggg gccagttcat
    14161 gcatacagac atgctggttt agcatctctt catctatctg atctggttgc aggtttcctt
    14221 gctgcacctt ttcagccagt tgcctgactc cctggactat atcccaacgt ccaccgtagt
    14281 tcgccgcaat attcagcgtc agaccggtat tcccagctgt tagcgcttca gatttacgaa
    14341 tacgttcttg caaacgcgag ttaaagcgac tggtatcgcc aataatacgc agacgcacgt
    14401 tatgtcggtg cagacttttt acttcgctat cgagcgccca cacaaacagt tccattaacg
    14461 cactgacttc ctgcgctggt cggttccagt tttcactact aaaggcatac agcgttaacg
    14521 cctcaatacc gttgttggcc gcaaaagaga cagcccggcg gacggatttt gccccggctt
    14581 tatgcccaaa ggcacgaatc ctcccttgct tttttgccca gcgaccattg ccgtccataa
    14641 tgatcgcaac atgacggcag ccatgcgctg gcaatttttc gctaagtggt tgagtagcag
    14701 acaacatcac gcgtttttta ttccctgaca gggtttacgg tactcaggaa tactgaagcc
    14761 tatacaaaaa aaagccgtgt aaaaccacgg catacctgac cttataaagc caactactta
    14821 cgatcagatg gcgcagacta tatcactgaa gccctacgct aacaaatagc gcgactctct
    14881 gtagccggat tatcctcagc ttgcgagacg catcacctct tttctggcga cttcacgcgc
    14941 gttcgcatca acagataaca catcgtccac acattgtggt tcgcgcatat ccattttttc
    15001 cagtacagat aaattcaacg cagcgatatc cgtaaagcgg atttgttgcg caagaaaagc
    15061 agcaacggtg atttcgtttg cggcattcaa tgctgtcgtc gctgcctgac cttgttcgaa
    15121 cgcctccatc gccagtttca ggcatggata acgatcataa tccggtgcgg caaatgtcaa
    15181 cgcacttagt ttgcaaaaat cgagcggctt cacgccagag ttcacgcgat tcggccatgc
    15241 catggtgtgg gcaattggcg tacgcatatc cggttccccc agctgcgcca gaacactgcc
    15301 gtcctgatag cgcaccattg agtgaatcac tgactgcggg tgaatcagca cttccatctg
    15361 gctggcgctg gcgttaaaca gccaacgcgc ttcaatgtat tccagacctt tgttcatcat
    15421 ggtagccgaa tcgacagaaa ttttacgtcc catcgaccag ttcggatgac ggcaggcttg
    15481 atcgggcgtc attgttgcca aatcgcgcaa tggcgtctca cggaaagggc caccagaccc
    15541 ggtaagtaaa atggacacca cgccattttg ctcaaggtca gcgtatccca gattatgctg
    15601 gataggttgc ggtaaactct gaaaaatggc gttatgttcg ctatcgaccg gtaacaattg
    15661 cgctttgctc tgctttacgg cgtccataaa cagacgtccg caggtaacca gtgattcttt
    15721 attggccagc aaaatggttt tacccgcgcg gatcgcagca agcgtaggta acagcccagc
    15781 agcgccaaca atggctgcca tcacctgatc aacatcctca agcgctgcca tatcgcaagc
    15841 ggcttgttgc ccacttaaga cttcggtgcg gctaccctgt tgctgtagca tcgttttaag
    15901 aagtttcgca ctcgcttcat cgtccattac agcatagcgg ggagagaatt ccaggcactg
    15961 ttctaccatg cgagtgacat ttttgcctgc caccagcgca actacgcgga agtgttcggg
    16021 attatggcgc accacgtcca gcgtgctgca accaatcgag ccggtcgagc ccagaatggt
    16081 gagttgcttc atgaaacatc cagagttgag acagaataaa aagcaaaacg ccgccagccg
    16141 atccgcaagg atctactgag cggcgttttt gtcgttcaag aaatcagaac tgcatcagtt
    16201 ctgcttcttt gtctgccagc gccgcttcaa ttttcttgat tgcagcatca gtcagtttct
    16261 gtacatcgtc ctgagaacgg cgatcgtcgt cttcgctgat ctctttatct ttcaacagtg
    16321 ctttcacttt gtcgttcgcg tcacgacgca cgttacgtac tgcaacacgc gcttgttctg
    16381 cttcaccacg aacgattttg gtcagatctt tacgacgttc ttccgtcagc ggcggcagcg
    16441 gaacacggat gtcgctaccc gcagagttcg ggttcaggcc aagatcggac gccataatcg
    16501 ctttttcaac ggccggagac attgaacgat caaacacgtt gattttcaga gtacgggaat
    16561 cttctaccgt tacgcttgcc agctgacgca gcggcgtcgg cgtgccgtaa tattccacga
    16621 caatgccatc cagcaggctg ggagaagcac gacccgtgcg tattttgctg atttgggttt
    16681 tgaacgcttc tacgcatttg tccatgcgta cttcagcatc ttttctgata tcgctaatca
    16741 cgttacgaat ccttgaaaac ttgtctcagt cagaccagac agtcacacac agtggaagtg
    16801 tgctaagtat agccctgatt aaacatatta cgggtaatcg tctggattat taggctattt
    16861 tatttgccat tttggccccg agcagtgctc gccatcctca cgtacttttg tacgctccgg
    16921 ttgctgcgcg ctggccgtga ccaaactgcc tgcaacaata acgccttata accagactta
    16981 cgcccccgcg atacttacgc ggaatcttac ccttatttat ccatcacggg aattattccg
    17041 tgattaaagt cccttctttt tcacccatta ccacacggcg cagcgcaccc ggtttgttca
    17101 tattgaaaac acgaatcggt aatttatggt cacgagccag cgtgaaggcc gccaggtcca
    17161 tgactttcag ctctttttcc agcacttcgc tgtaagtcag ttgctcgtac atggttgcgg
    17221 ttggatcttt cgccggatca gcggtaaaca cgccgtcaac tttggttgct ttcagcacca
    17281 catcggcttc aatttcgata ccacgcaggc aagctgctga gtcggtggta aagaacgggt
    17341 tacctgtacc ggcggagagg atcaccacac ggttgttgcg caacaggctg atagcttctg
    17401 cccagctgta gctgtcgcac acgccattca atggaatagc ggacatcaga cgagcgttca
    17461 cataggcgcg gtgcagtgca tcacgcattg ccaggccgtt cattacggtc gccagcatcc
    17521 ccatgtggtc gcccacaacg cggttcatac ccgctttcgc cagaccagcg ccacggaaca
    17581 ggttaccccc accaatcacc acaccaacct gaatacccag ttcaaccagt tctttgattt
    17641 cctgagccat acgatccagt atgcttgcat caataccgaa gccttcagtg ccctgcagag
    17701 cttcgccact caacttaagc agaatgcgtt tatagacggg ttttgcattg gtagccatgt
    17761 ttctttcctg agactgtcaa cgattgaaat gaattaatac tagcgacatt gtacgtcgct
    17821 tttcagcgca gccccacaag ggttagctga atttacaaga tgggcacaaa aagaagccgc
    17881 cctcaggcgg ctccttttcg ataattaaga ctgcttggac atcgcagcaa cttctgctgc
    17941 aaagtcagtc tcaactttct cgatgccttc ccccacttcg aagcggatga agccagtcac
    18001 ttcagcgtta tgctctttca gcagctgacc aacagttttg cttggttcca taacgaacgg
    18061 ctgaccggtc agagaaactt cgccggtgaa tttcttcatg cggccttcaa ccattttctc
    18121 tgcgatttct ttcggcttac cagactgcat cgcgatatcc agctgtacct ggtattcttt
    18181 ttctaccact tcagcggata cgtcttctgg tttgatgaat tctggcttgc ttgcagcaac
    18241 gtgcatagcg atgtgtttaa ccagctcttc gtcagcgcct ttagcagcaa ccagaacgcc
    18301 gatacgcgca ccgtgctgat aagaacccag aacgtcgcct tccagcgcag caacgcggcg
    18361 aatgttgatg ttctcaccaa ttttcgctac cagcgcaaca cgttcttctt cgaactgtgc
    18421 tttcagaact tcaacgtcag tgattttgcc agcaacagct gcgtccagaa ctttgtctgc
    18481 gaacgcctgg aaaccagcgt cttttgcaac gaagtcagtc tggcagttaa cttccagaat
    18541 gatgccgtag ttgccgtcga ttttggtttt gatcacgccg tcagcagcaa cgttgcctgc
    18601 ttttttcgct gctttaatag caccggactt acgcatgttt tcgattgcca gctcgatgtc
    18661 gccgttagct tcagtcagtg cttttttgca atccatcatg cctgcgccag tacgctcacg
    18721 cagctctttt accagggatg cggtaatttc agccattcta aaatcctcgg agatgtgatc
    18781 tgcccggcat taaaccgcac agatataaaa gtgaaaaagg gggccatatg caggccccct
    18841 aaccaaacgt gtactacctg gtctataagg gctctccccc ttcatatttt ttcgcgccgc
    18901 agatgcgttg tcttcgctcg ctcatcccgg tcacttactg atgtaagctc ccgggaattc
    18961 tcgagcttgc cgcctttctg caactcgaac tattttgggg gagttatcaa gccttattac
    19021 tcagcttcta cgaagctttc ttccgcctgg gaagccagat cctgagaacg gccttcacgt
    19081 acggttgcag caacagcgcc caggtacagg gtcacagcac ggattgcgtc gtcgttaccc
    19141 gggataacga agtcaacacc gtccggatca gagttggtat caacgatagc aaataccgga
    19201 atacccaagt tgtttgcttc tttgatagca atgtgttcgt ggtcagcatc gattacaaac
    19261 agagcgtccg gcagaccgcc catgtctttg ataccgccca ggctgttttc cagtttctcc
    19321 agctcacgag tgcgcatcag cgcttctttc ttggtcagct tttcgaaagt accgtcctga
    19381 gactgagttt ccaggtcttt cagacgtttg atggactgac gaacggtttt ccagttagtc
    19441 agcataccgc ccagccagcg atggttcacg aagaactggt cgcagctcag agcagcgtct
    19501 ttcaccgctt cgcttgcagc gcgtttagta ccaacgaaaa ggattttacc tttgcgagaa
    19561 gcaatcttgt tcagttcagc cagagcttcg ttgaacatcg gtacagtttt ctcaaggttg
    19621 atgatgtgaa ctttgttacg cgcaccgaag atgaacggct tcattttcgg gttccagtaa
    19681 cgggtctggt gaccgaagtg aacaccagcc ttgagcatgt cgcgcatgga aacagttgcc
    19741 atgattaaaa cctctatata aaagttgggg ttatgcctcc acgtatccca tattaccgac
    19801 cccaaagggc accccggaat atgtgccgat acgtgtgtgt tgttacacaa agtgagattg
    19861 tcgcttccgt ccagtctgtg tatacgaaat ggatcggaag tccggcgcgc tttataccac
    19921 aaatacgtca tggacaccaa taattgttgg cgctgtgtac agcatcagac gttgaatttt
    19981 ctattataga aaacgttcag tggcacgttt ggcgaaattc agaatgattc tcaatttgcc
    20041 cgggtgtgat accattgacg gcacttacat atatattgtc ggtatcaccg acgctgatgg
    20101 acagaattaa tggctatctc aatcaagacc ccagaagata tcgaaaaaat gcgcgtcgct
    20161 ggccgactgg ctgccgaagt gctggagatg atcgaaccgt atgttaaacc gggcgtcagc
    20221 accggcgagc tggatcgcat ctgtaatgat tacattgtta atgaacaaca cgcggtttct
    20281 gcctgcctcg gctatcacgg ctatccgaaa tccgtttgca tctctattaa tgaagtggtg
    20341 tgccacggta tcccggacga tgcgaagctg ctgaaagatg gcgatatcgt taacattgat
    20401 gtcaccgtaa tcaaagatgg tttccacggc gatacctcga aaatgtttat cgtcggtaag
    20461 ccgaccatca tgggcgaacg tctgtgccgc atcacgcaag aaagcctgta cctggcgctg
    20521 cgcatggtca aaccaggcat taacctgcgc gaaatcggtg cggcgattca gaagtttgtc
    20581 gaagcagaag gcttctccgt cgttcgtgaa tattgcggac acggtattgg tcgcggcttc
    20641 catgaagaac cgcaggtgct gcactatgac tcccgtgaaa ccaacgtcgt actgaaacct
    20701 gggatgacgt tcaccatcga gccaatggtc aacgcgggta aaaaagagat ccgcaccatg
    20761 aaagatggct ggacggtaaa aaccaaagat cgcagcttgt ctgcacaata tgagcatact
    20821 attgtggtga ctgataacgg ctgcgaaatt ctgacgctac gcaaggatga caccatcccg
    20881 gcgataatct cgcacgacga ataagatgaa gccggcgaat gccggctttt ttaatgcgat
    20941 aatttaatct tatgggtggc gcacaatgaa tacccttcca gaacagtacg caaacaccgc
    21001 tctccccacc ctgcccggtc aaccgcaaaa tccatgcgtc tggccccgtg atgaattaac
    21061 cgtcggtggg ataaaagccc atatcgatac tttccagcgt tggctgggtg atgcctttga
    21121 caatgggatc tctgcagaac agttgattga ggcgcgcacc gagtttatcg accagctcct
    21181 gcaacgatta tggattgaag cgggattcag ccagattgcc gacctggcat tggtcgccgt
    21241 cggtggctac ggtcgtggcg agctgcatcc actttctgac atcgatttac tgattttaag
    21301 ccgtaaaaag ctcccggacg atcaggcgca aaaagtgggc gagctgttaa cgctgctctg
    21361 ggatgtaaag ctggaagtcg gtcatagtgt gcgcacgctt gaagagtgca tgctggaagg
    21421 gttatcggat ttaaccgtcg ccaccaattt aatcgaatcc cgcttattaa ttggcgatgt
    21481 tgcgctcttc ctcgaactgc aaaaacatat ttttagcgaa ggattctggc cttccgacaa
    21541 gttctacgcg gcgaaagttg aagaacagaa ccagcgccat cagcgttacc acggcaccag
    21601 ctacaatctt gaaccagaca tcaaaagtag ccctggcggc ttgcgcgata tccacactct
    21661 gcaatgggtg gcccgccgtc attttggcgc aacatcgctg gatgaaatgg ttgggtttgg
    21721 cttcttaacc tcagcggagc gggcggaatt aaacgaatgt ctgcatatac tgtggcgtat
    21781 tcgctttgcc ctgcatctgg tcgtcagccg ttacgataat cgcctgttat tcgatcgcca
    21841 acttagcgtc gctcagcgtc tgaattacag tggtgaaggc aacgaaccgg tcgagcggat
    21901 gatgaaggat tacttccgcg ttacacgccg cgtcagtgaa ctcaaccaga tgctgctgca
    21961 actgttcgat gaagccatcc tcgcccttcc cgccgacgaa aaaccacgtc caatcgacga
    22021 tgagtttcag ctacgcggta cgctaatcga cctgcgtgat gaaacactat ttatgcgcca
    22081 gccggaagcc atcttgcgta tgttctacac catggtgcgc aacagtgcga tcaccggcat
    22141 ttactccacc acgctgcgcc agttacgcca tgcccgtcgc catctgcaac aaccgctgtg
    22201 taatattccg gaagcacgaa aactgttttt gagcattctg cgtcaccccg gagcggtgcg
    22261 gcgcgggcta ttgccaatgc atcgccatag cgtgctcggc gcgtatatgc cgcaatggtc
    22321 gcatatcgtc gggcagatgc agtttgatct gttccacgcc tacacggtgg atgaacatac
    22381 tatccgcgtg atgctgaaac tggagagttt tgccagtgaa gaaacgcgcc agcgccatcc
    22441 gttgtgtgtg gacgtctggc cgcgcctgcc gtcaactgag ctgattttca tcgccgcgct
    22501 gtttcacgat atcgccaaag gacgcggcgg cgaccactcc attctcggtg ctcaggatgt
    22561 agtgcatttt gcagaactcc acgggctgaa ctcacgcgaa acacagctgg ttgcctggct
    22621 ggttcgccag cacttgctga tgtcggtgac agcacaacgc cgcgatattc aggaccctga
    22681 agtcatcaag cagtttgccg aagaagttca aacggaaaat cgtctgcact atctggtatg
    22741 cctgacagtg gctgacattt gcgccaccaa cgaaacgctg tggaatagct ggaagcaaag
    22801 tctgttgcgc gaactctatt ttgccaccga aaagcagcta cgacgcggaa tgcaaaacac
    22861 gccggatatg cgcgaacggg ttcgccatca ccaactccag gcactggcac tactgcgcat
    22921 ggataacatc gacgaagagg cgctgcacca aatttggtca cgctgtcgtg ctaactattt
    22981 tgtccgccat agcccaaatc aactggcctg gcatgcccgc catttattac agcatgattt
    23041 aagcaaaccg ctggtattgc ttagcccgca ggctacgcgt ggaggcaccg agatttttat
    23101 ctggagcccg gaccgcccct atctgtttgc cgccgtctgt gccgaattag accgccgcaa
    23161 tttaagtgtt cacgacgcac aaattttcac cactcgcgac ggtatggcga tggatacctt
    23221 catcgtgctg gaacccgatg gtagcccgct atccgcagat cgtcatgagg ttattcggtt
    23281 tggtctggag caggtactga cacaaagtag ctggcagcca ccacagccgc gtcgtcaacc
    23341 ggctaaacta cgtcacttta ctgttgaaac cgaagtgacg tttttgccaa cccataccga
    23401 ccgcaaatcg ttccttgagc ttatcgcgct cgaccagcca ggactgctgg cgagagtcgg
    23461 gaaaattttt gccgatctgg gaatttcgct tcatggtgcc cgaattacaa ccattggcga
    23521 gcgagtagaa gatttattca taattgccac cgctgaccgg cgtgcactta ataacgagtt
    23581 gcagcaggaa gtgcatcagc ggttgacaga ggccctcaat ccaaacgata aagggtgatg
    23641 tgtttactga tatgaaaaga gtttaacaat gcagcagtta cagaacatta ttgaaaccgc
    23701 ttttgaacgc cgtgccgaga tcacgccagc caacgcagac accgttaccc gcgaagcggt
    23761 aaatcaggtg atcgccctgc tggattccgg tgcactgcgt gtagcggaaa aaattgacgg
    23821 tcagtgggtg acgcatcagt ggttgaaaaa agcggtgctg ctctctttcc gtattaatga
    23881 taatcaggtg atcgaagggg cagaaagccg ctacttcgac aaagtgccga tgaaattcgc
    23941 cgactacgac gaagcacgtt tccagaaaga aggcttccgc gttgtgccac cagcggcggt
    24001 acgtcagggt gcgtttattg cccgtaacac cgtgctgatg ccgtcttacg tcaacatcgg
    24061 cgcatatgtt gatgaaggca ccatggttga tacctgggcg accgtcggtt cttgtgcgca
    24121 gattggtaaa aacgtccacc tttccggtgg cgtgggcatc ggcggcgtgc tggaaccgct
    24181 gcaggctaac ccaaccatca ttgaagataa ctgcttcatt ggcgcacgct ctgaagtggt
    24241 tgaaggggtg attgtcgaag aaggttccgt catttccatg ggcgtataca ttggtcagag
    24301 cacccgtatt tacgaccgtg aaaccggcga aatccactac ggtcgcgttc cggcggggtc
    24361 tgtggttgtt tcaggtaatc tgccgtcaaa agatggcaaa tacagcctct actgtgcggt
    24421 tatcgttaag aaagttgacg cgaaaactcg cggcaaagtc ggcattaacg aactgctgcg
    24481 taccatcgac taaaagtatg cacacgggca gcacgacgct gcccgatttt ttttgcaggg
    24541 atgagcgatg atttcacgcc gccgattgtt gcaggctact gccgcgacga tagccacgag
    24601 ctcaggtttc ggttatatgc attactggga gctaggctgg tttgaattaa tccgtcaccg
    24661 cctcgccttt tttaaagaca acgcagcacc attcaaaatt ctttttctgg ccgatctcca
    24721 ttactctcgt tttgttcctt taagcctgat ttctgacgcg attgctcttg gcatagaaca
    24781 aaagcccgat ttgatattac tgggcggcga ttacgtatta tttgatatgc cgctgaattt
    24841 ttcggcgttt agtgatgtcc tctcccccct tgccgaatgt gcgccgacgt ttgcctgctt
    24901 cggcaaccac gatcgccctg ttggtacaga aaaaaatcac ttaattggcg agacgttgaa
    24961 atcagtgggc atcacggtgt tgtttaacca ggctacggtg atcgccacgc cgaacaggca
    25021 attcgaactg gtaggcaccg gtgacttatg ggccggacaa tgcaaaccac ctcccgccag
    25081 cgaagcaaat ctgccaagac tgatgctggc acataatccc gacagcaaag aagtcatgcg
    25141 tgatgaaccc tgggatctga tgctgtgcgg ccatactcac ggcgggcaac tgcgcgtacc
    25201 gttggttggt gaaccttttg cccctgtcga agataaacgt tacgtcgccg gattaaatgc
    25261 ctttggcgaa cgacacatct acacaacccg tggcgtgggc agtttgtatg gtttgcgtct
    25321 gaactgccgc ccggaagtaa cgatgctgga actggtgtag aacgtgaatt ttatcaccac
    25381 aataaagcag gtaatttttt gatttttacg ccatgataga agtctggaag aaaaaggctt
    25441 tctgcgatca cagtcgagcc gggcgggcca tgccaaatgc taaaataagc acaatttact
    25501 gactcatttt gaggtaacgc tatgtacgac aatctgaaaa gtctgggtat taccaatcct
    25561 gaagaaattg atcgttacag cctccggcag gaagccaaca acgatattct gaaaatctat
    25621 ttccagaaag acaaaggcga gtttttcgcc aagagcgtga agtttaaata tccgcgtcag
    25681 cgtaaaacgg tcgtcgctga tggtgtgggt cagggttata aagaagtcca ggaaatcagc
    25741 ccgaatctac ggtatatcat tgatgagctt gatcaaatct gccagcgtga ccgcagcgaa
    25801 gttgatctta agcgtaagat cctcgacgac ttacgtcacc tggagtcagt cgtaaccaat
    25861 aagatcagcg agattgaagc cgatctggaa aaactaacgc gtaaataatg ccgaactgac
    25921 ggttaactcg gacattcccc tctacccttc gggctgaggg gaatgagcca gcgccgcctc
    25981 gaccttaacg cataacctac cgctcttcat ccagttgtaa cgccacatac agcagcaacc
    26041 tgtcatcaaa attacccaaa tcaagcccgg tcagttccga tatacgatta agccgatact
    26101 ccagagtatt acgatgaata aacagcgcct ttgacgtagc cagcggttgc acattgtggc
    26161 gaaaccacgc cgccagcgtt cgtcgcagca agccattatt gtccatcgct ttcagccgcg
    26221 ccagcggtcg cgccagttcg ttggcctgcc agtcgccacg caaactgtcg agtaacacag
    26281 gtaacatcag atcctgataa aaatagcagc gactttctgg catccgctgt ttacccacca
    26341 ccatcgtcgt tttcgccgta cgataggatc gggcaatact gccaggaccg gtaaaatagt
    26401 tgcccagtga aacgcgaaaa cgcagctggc cgtactcttt catgcgggta atcagttgtt
    26461 caactcgctt acgatgatct tctgcatccc agcgcccaaa agagttcaac gccggtttca
    26521 acaccaccat ttcggtcagc gaaacaattg ccaccagatt attacgctcg ggcgtagtca
    26581 gcgcattttg cagttgttgt aactccgcca ttgcgctgtc cacgccaagc tgaccgctgt
    26641 cgacctcaac aatagccacc actcgcggtt gattgagatc aatccccagc cgttgcgccc
    26701 attcagtaag tgcgggagta ttctcctcag cctgaatcag gttcatcacc agttcttccc
    26761 gcaaacggct atcctgcgcc aacaggtgca tcaaccgcga ctgttccagc atcatttcag
    26821 ccgtcatgca gaccagttcg ccatatttac gcagattctc tggttcacct gtcaggccaa
    26881 ttacgccgac aatttcacct tccagccgta acggtagatt aatcccctgc cgcacaccgt
    26941 gcagatgacg tgctaccgcg tcatcgatat cgacgactcg tccctgtgaa agtaccagca
    27001 atgcaccttc gtgcaattca ccaatacgct cacgatcgcc gctgccgata attcgcccgc
    27061 gggcatccat tacgttgata ttggtatcga tgatgcgcat ggtacgtgcc acgatatcct
    27121 gcgccatttt ggtatcaaga tgccagccag ccataagtcc tccgttatgc acggcttagc
    27181 ataaggaagt acgtaacgta cggcattgtg caaatgccca aaggatgatg aagatgtatg
    27241 gagttgtggc gaagttcaca gattgtaagg agaacccctt cccgtattca ggaaggggtt
    27301 gagggagatt actgcattaa caggtagatg gtgctgtcgc cgcgctgaat gttcagtgcc
    27361 agcacagacg gtttgctgtc gagcacttta cgcagttcag cgatgttttt cactgcctgc
    27421 tggttcgcgc caataatcac atcacctttc ttcaggccga tctgcgcagc cggagtgccc
    27481 gttttcacgt tgttcactac cacgccctga tctttgcctt tgttgctcat ctcagcgcct
    27541 tcaatgccgt tgaagatgga gctggaatca acctgattct ggctgctctg ctgcagttcc
    27601 aggttcacgt taacctgctt accgtcgcgc agtaagccca gggtcagttt gctgcccacc
    27661 ggcatagtac ccacctgagc acgcagtgcg gcaaagctgc tgatcggctt accgttcagt
    27721 gaggtgatca catcacccgc tttaatgccc gcttttgcag cggaggaatt aggcagaacc
    27781 tggcttacga aagcaccgcg ctgggcgtca actttcatcg ctttggccag ttcggagttc
    27841 agctcagtcc ccataatccc cagctcaccg cgtttcacct ggccgtattc caccatctgc
    27901 gaggtcaggt ttttcaccat gttactcggg atagcaaaac cgataccgat gttgccgccg
    27961 tccggtgcga ggatcgcggt gttgataccg atcagttcgc cgttcaggtt aaccagcgca
    28021 ccaccggagt taccacggtt gatcgctgca tcggtctgga tgaagttttc gtagttttca
    28081 gcattcaggc cgctacgccc cagcgcagag acaatcccgg aagttaccgt ctcgcccaga
    28141 ccaaacgggt taccaatcgc tacggtgtaa tcacccacgc gcagtgcatc agaatccgcc
    28201 atcttaattg cggtcaggtt tttcgggttc tggatttgga tcagcgcgat atcagagcgc
    28261 ggatctttgc caaccatctt cgcgtcgaac ttacgaccat cgctcagctg gactttaatc
    28321 accgtcgcgt tatcaacaac atggttgttg gtgacgacat agcctttatc ggcatcaatg
    28381 atgacaccgg aacccagcgc catgaatttc tgttgctggc cgccgccatt accgccctgg
    28441 cccccctggc agaacggaga gctctgaaac ggtgaacctt cctggcagaa cggagaatca
    28501 tcaccgaaga actgctggaa attacgcggc atacgcggcg tattaacggt tgtgctacct
    28561 tctacgttaa tgctgaccac tgaaggcatc accttttcga gcatcggtgc aaggcttggc
    28621 atctgctggg ctgtcgttgc tgaagaagtc tcagccgccg ttgcagagag cggagataac
    28681 gccaaaccta aactcagagc cagtgcactc agtgctaatg tggttttttt catgtatttc
    28741 agtctcgatt aacagataac gcaaaattgc tgtgttcttc agattcgttt tatagcgcga
    28801 agttccgaac taaagtttct gcaaaaggta aaaatttatt gttcgtcttt acaaaagcct
    28861 ggttattgtt ctacggccat cagacgtcgg tattcatccc acgcatagag gtcggtcata
    28921 ccgctgatat aatcctgcag taggcggcaa cggtaataat attcccatag cggaaactca
    28981 ggagaatctg acggtaattt actgacagcc tcgacatagg ccagccgatg gcgcgtcgag
    29041 agtttgtgga ataagcgcga ctcaataggg aaacgtttca cccgttcttt ttctaccagt
    29101 tcagtaaagt ctgataacga caggcttaat aaaggacgat aaatctctaa taatccgcta
    29161 atgacccgat agccctgcaa ttcaagctgc tcgacatccg gatggctaaa cacatgtttt
    29221 acagcgacat ttttatatag cttaagaaga tcgctgcatt cgctggcatc ttccaataat
    29281 gcatgattaa acgttccggc gaaaatcgca ggcagattat caataaatcg ttgtgccgcg
    29341 tagggtacca gtttatttag ggtgtttacc cgtaaataca taaaaaactg atcttccgta
    29401 ctgcggctta aactatttga gcgtgatttt tcccaggcat tttcaaccac tagcgaaaag
    29461 agcgaacctt tctcatgctg accccacgcc tcgtgcaaat gatgataaag ctgctcaacg
    29521 gtaaatattc ttttctctac cgcatcttca aggtctgcca cacaatagga gatgtcgtcg
    29581 gcagcttcca taatccacgt taatggaaaa cgactgtaaa gcgccaaatt aagttcttta
    29641 cgcaaccggg caatataggc ttcttcagaa agataataac ccggcttttt cattaaatag
    29701 tgatgtgtct caggcgtttc gccacgccac cacgccggac gggtatattt taaaataccg
    29761 ccaacctgtg cccaagtgag attcatccgc atcaatgtat gcaccagacg aatgccttgt
    29821 gcattcccct caaaatgaca taagtcctga cgaatcttgc gccgcagctc gttaagctgt
    29881 tcttccccgt cccgtaaacg tagtgccgcc acgctgcagc gatcgtcagt cagaggctgg
    29941 ctttcggcat cttccgggtg caaacgttgg cgaaaccagt catttatcgc tgcttcgcca
    30001 aaatgaccaa acggcggatt gccgatatcg tgcatcaggc atgacatctc aacaatgctt
    30061 tcaaaggggc cggtcagttc atccaggccg tatgcttcca gcaatttaag ctctttcaga
    30121 cggcttaaaa tttctttggc gatgtagcgt cccacctgct ggacttccat cgagtgggta
    30181 agacgcgtgc gcacggcggc attgcgctcc agtggaaaaa cctgggtctt ttgttgcaga
    30241 cgacgaattg ccggagagtt gatgatacgc ccgcgatcgc tctcgaagat ccgcaggatc
    30301 tcatgttcgg ttttaacgcc ctgcggtgaa cggtaacgac gatgccagtt tatttttttt
    30361 cggaaatcaa tctgtgccat gtcctctccc gcgtgagaaa tacgcttccc cgtaagcgca
    30421 tggtaaacta tgccttcaaa tcgggcttat cgcgagtaaa tctatgaaaa tcggcatcat
    30481 tggtgcaatg gaagaagaag ttacgctgct gcgtgacaaa atcgaaaacc gtcaaactat
    30541 cagtctcggc ggttgcgaaa tctataccgg ccaactgaat ggaaccgagg ttgcgcttct
    30601 gaaatcgggc atcggtaaag tcgctgcggc gctgggtgcc actctgctgt tggaacactg
    30661 caagccagat gtgattatta acaccggttc tgccggtggc ctggcaccaa cgctgaaagt
    30721 gggcgatatc gttgtctcgg acgaagcgcg ttatcacgac gcggatgtca cggcatttgg
    30781 ttatgaatac ggtcagttac caggctgtcc ggcaggcttt aaagctgacg ataaactgat
    30841 cgctgccgct gaggcctgca ttgccgaact gaatcttaac gctgtacgtg gcctgattgt
    30901 tagcggcgac gctttcatca acggttctgt tggtctggcg aaaatccgcc acaacttccc
    30961 acaggccatt gctgtagaga tggaagcgac ggcaatcgcc catgtctgcc acaatttcaa
    31021 cgtcccgttt gttgtcgtac gcgccatctc cgacgtggcc gatcaacagt ctcatcttag
    31081 cttcgatgag ttcctggctg ttgccgctaa acagtccagc ctgatggttg agtcactggt
    31141 gcagaaactg gcacatggct aagtcactgt tcagggcgct ggtcgccctg tcttttcttg
    31201 cgccactgtg gctcaacgcc gcgccgcgcg tcatcacgct ttctcccgcc aacactgaac
    31261 ttgcctttgc cgccgggatc acgccggttg gggtcagcag ctattccgac tatcctccac
    31321 aagcgcaaaa gattgagcag gtttccacct ggcaggggat gaatctggaa cgcattgtcg
    31381 cgctgaaacc cgatctggtg attgcctggc gtggaggtaa tgccgagcgg caggttgacc
    31441 agctggcttc gctgggaata aaagtgatgt gggtcgatgc gacaagcatt gaacaaattg
    31501 ccaatgcgtt acgtcaattg gccccctgga gtccgcaacc ggataaggcc gaacaagccg
    31561 cgcaatccct gctggatcag tacgcgcaat tgaaagcgca atatgctgat aaacctaaaa
    31621 aacgtgtttt tctgcaattc ggcattaatc cgccatttac cagtggaaaa gagtcgattc
    31681 aaaaccaggt actcgaagtt tgtggcggag aaaacatctt taaagacagc cgggttccct
    31741 ggccgcaagt tagccgcgaa caggtgttag cacgctcgcc acaggcgatt gtcattacag
    31801 gcggaccgga ccaaattcct aaaatcaaac aatactgggg tgaacagctc aaaattcccg
    31861 ttattcctct cacgagtgac tggtttgaac gtgcaagccc acgtattatc ctcgctgcac
    31921 aacagctctg taatgcgctt tcacaggtag attagcgcct gaccttttta ggtgctcagt
    31981 ggggattgaa caatgctcgt ctattggctg gatatagtcg gcacagcggt atttgccatc
    32041 tccggcgttt tgttagccgg aaaattgcgt atggaccctt ttggtgttct ggtactgggc
    32101 gtggttaccg ctgtaggcgg cgggacaatt cgcgacatgg cgctggatca cggcccggta
    32161 ttttgggtga aagatcccac cgatctggtc gttgcaatgg taaccagcat gctgaccatc
    32221 gtgctggtgc gccagccaag acgcttacca aaatggatgt tgccggtgct ggacgccgtt
    32281 ggtctggcgg tgtttgtcgg cattggcgtg aataaagcct ttaatgcgga agtcggtccg
    32341 ttaatcgcgg tttgtatggt cgtcattact ggcgttggcg gcgggatcat tcgtgatgta
    32401 ctggcccgcg agatccccat gattttacgt acagaaatct acgcaactgc ctgtattatc
    32461 ggcggtattg tccacgctac ggcttattac acattttccg taccactgga aacagccagt
    32521 atgatgggca tggtcgtgac gctattgatt cggctggcgg ctattcgttg gcatcttaag
    32581 ctaccgacgt ttgcgctgga tgagaatggg cgttgaagag agaagatgcg ccggatagtt
    32641 tatccggcac atgaacaaca gattagatgc taaaggaaga accgcaaccg caggtgcttt
    32701 tcgcgttcgg gttggtcacg atgaaacgag aaccttccag tccttcggta tagtcaacgg
    32761 aaccgccgac cagatattgc aggctcatcg gatcaaccac caagccaacg ccctgttttt
    32821 cgatggtcat atcgccttcg ttcacctgat catcaaaggt gaaaccatac tggaagccgc
    32881 tgcaaccgcc accggtgata tacacgcgta atttcagatt cgggttatct tcgtcagcga
    32941 tcaggctttt aactttgttg gctgctgcgt cggtaaactc cagcggcagt gctacgtcat
    33001 cactcatatt ttgctccaaa cgacatcggc aattgggcaa attctaaccc aataataatc
    33061 gccattatct aataccctgg tatttcgttc aagtattctc gctggctgat gcggccttgc
    33121 ttcgcgccag ttgctcagct tcctgttttg ccagcgtgcg cgcaagaatc gccgagtata
    33181 gcggtttccc gccggtaaat tgcgctaata gtgttgcgcc aagaccggta ataatcattg
    33241 gcaaaatgag ctggtagtta tcggtcatct ccagaaccag aatgatcccc gttaacggcg
    33301 cgcgaataga tgccgccagt aatgccccca ttccggcaat agcaaacgtc cccgcctcaa
    33361 ggtgatattg cggaaacagc tcaacggcaa ccattccgaa agcggttccc agcacagtac
    33421 ccagcgccag catcggggca aaaataccgc ccggcgcgcc ggaagagaag cagagtaagg
    33481 tggtaatgac ccgagcgacg aagataaaca ccagcattcc catgctgaaa ttccccgcgg
    33541 tagcgatagg aatcaggtta aaaccgccgc ccgacgttgc tggtgccaca aaccccagca
    33601 atccacacag accgccaatc gcaccgccca tcagtaccca tttggtaata ttgccgccgt
    33661 gcacacggtg cagcaaatcc tgcatcccca gcacccattt attaaaaata gggccgaaaa
    33721 tgccaaaaat aataccgagg atcagataaa gccacagcgt attaagcggc gcatcagaaa
    33781 gtttaccgac gtcaatcaac gcaacttcat gattaaaaat ccggtacata atggtcgaca
    33841 taatgacgcc aataaatacc gcttttatcg aaattaacgt atagcgaaac tgcggacgca
    33901 tctcttcgat aataaacaaa atacccgcca gcggcgcgtt aaaggccgca gccagccccg
    33961 cagcagcacc ggttgccagc agcgtatggc gagcttcgtc acctttcagg cggaaaatat
    34021 caagcaccat acggccaatg ttaccgccga tctgcacggt tggcccttcg cgccccaaca
    34081 ccatgcctcc gccgagtgtc cccagcccgc caaagaactt caccggcaat acacgccacc
    34141 agcgaacggg acgttgatct tccagcgccc cttcaatttc cgggatcccc gaaccacctg
    34201 cttccggcgc gtatttgcgc accaaaaagt agccaaacat cgccagcacc gccgaacaga
    34261 gaaaagcgac ggttaacaga agcggataat tatcagcagt atgtaccagc gcccccatac
    34321 gttggttctg caaccaggcg acacctttgt caaaagcaac cgctgccagc ccaacaagcg
    34381 tgccgacgac tgccgccata aacaaaatgg ctaacggggt tttatcgcgc tcaagaagtt
    34441 ggcgaatcag ttgtctgcgt cgcaggcgcg cggcctgcgg tgtttctaaa gagggagtat
    34501 cagttttcat ccaatgatca cttattggtc atacaaataa gatgacggca ttttactcgc
    34561 acgttccatg aaaatccttt gcaaagcgta atgtttcaaa tgcacaaaac ttttatactc
    34621 taagaaatca ggattgcacc agtacaagca gcctgatgtt tgacgagtat ttaacttgtt
    34681 atgaataaca tagaatagca gccattcact ttttctacga atcaggaacc ctccatgagt
    34741 aagtctgaaa atctttacag cgcagcgcgc gagctgatcc ctggcggtgt gaactcccct
    34801 gttcgcgcct ttactggcgt gggcggcact ccactgttta tcgaaaaagc ggacggcgct
    34861 tatctgtacg atgttgatgg caaagcctat atcgattatg tcggttcctg ggggccgatg
    34921 gtgctgggcc ataaccatcc ggcaatccgc aatgccgtga ttgaagccgc cgagcgtggt
    34981 ttaagctttg gtgcaccaac cgaaatggaa gtgaaaatgg cgcaactggt gaccgaactg
    35041 gtcccgacca tggatatggt gcgcatggtg aactccggca ccgaggcgac gatgagcgcc
    35101 atccgtctgg cccgtggttt taccggtcgc gacaaaatca ttaaatttga agggtgttac
    35161 cacggtcacg ctgactgcct gctggtgaaa gccggttctg gcgcactcac gttaggccag
    35221 ccaaactcgc cgggcgttcc ggcagatttc gccaaacata ccttaacctg tacttataac
    35281 gatctggctt ctgtacgcgc cgcgtttgag caatacccgc aagagattgc ctgtattatc
    35341 atcgagccgg tggcaggcaa tatgaactgt gttccaccgc tgccagactt cctgccaggt
    35401 ctgcgtgcgc tgtgcgacga atttggcgca ttgctgatca tcgatgaagt aatgaccggc
    35461 ttccgcgtgg cactggctgg cgcacaggat tattacggtg tggaaccgga tctcacctgc
    35521 ctgggcaaaa tcatcggcgg tggaatgccg gtaggcgcat tcggtggtcg tcgtgatgta
    35581 atggatgcgc tggccccgac gggtccggtc tatcaggcgg gtacgctttc cggtaaccca
    35641 attgcgatgg cagccggttt cgcctgtctg aatgaagtcg cgcaaccggg cgttcacgaa
    35701 acgttggatg agctgacaac acgtctggca gaaggtctgc tggaagcggc agaagaagcc
    35761 ggaattccgc tggtcgttaa ccacgttggc ggcatgttcg gtattttctt taccgacgcc
    35821 gagtccgtga cgtgctatca ggatgtgatg gcctgtgacg tggaacgctt taagcgtttc
    35881 ttccatatga tgctggacga aggtgtttac ctggcaccgt cagcgtttga agcgggcttt
    35941 atgtccgtgg cgcacagcat ggaagatatc aataacacca tcgatgctgc acgtcgggtg
    36001 tttgcgaagt tgtgatccag catggtttgt cggatgcggc gtgaacgcct tatccgacct
    36061 acgattcggg gccgtttgta ggcccgataa gacgcgccag cgtcgcatcg ggcatccgtt
    36121 gtcgcgtaaa cgctttatcc gtccaggcgt acagggccgt tatatggaaa aattaacggc
    36181 tctgctttct cagcaaatag ataaaatatg gcgcgccgat aaaggtcgac agcagccccg
    36241 ccgggatctg gaatggaaac agcaccatcc gcccacacca gtcagcgaat accagcagta
    36301 aaccacccac cagcgccgaa attacgatgt gtggcatcgt ccgtcgaaag cccatcatcc
    36361 gcgcaatatg cggtgccatt aaaccaacaa aactcaacgg tccaatagtc atcgtcgcgg
    36421 tcgccgtcag gcaagccgct aacagcagca gcgcaattcg cgtcggcgtc agcgccattc
    36481 ctacggctcg tgcggtatca ccacccagcg gtaaaatggt cagccagcgg cggtacagcg
    36541 gggtaatcgc cagcaaaatc accatcacaa ttccggtacg ccagacctgc gcatcggtcg
    36601 cgttgtaggt cgaaccggaa atccaggtca gcacttgcgc cattcgcggg tcaccgctcg
    36661 cctgcaacat catcaaaagc atggtgaatg cggtacttaa cgccatcccc gccagtaaca
    36721 tacggtgtgg ggaaaatcca ccgcggccgg cggcgatcat aatgatcaac agcgtcaccg
    36781 ccgccccgat actgcccgcg ggtaacagcc agccaaaggc attacccggc accagaaaca
    36841 gcattaacac cacgccaaac gccgcgccag agctaatccc cagcacttcc gggcttgcca
    36901 tcgggtttcc ggtcagccgc tgaataatac agcccgccac cgccagcatg acgcccgcaa
    36961 acagcgccgc cataattcgc ggccagcgcc agggcatcaa atcctcgagc aacgccccgc
    37021 tcgcccacgt ccagccgtgc gcatcacgac caaacgacag cgccaccacc acagccatca
    37081 acagcagcac gccgcccgcg agggcaaacg ccagcacatg ttggcgttca gccgcgacac
    37141 gatcgttgac cttcatatcc ggcgcgctaa tgctgcgtaa acgcggcaac agccacagta
    37201 gcagcggcgc accgatcaac gcagtgaccg aaccggtgga cacttccatc cacacgcgag
    37261 tcagccagag gatgatttga tcggaaagcc agaggatcag cgccccaatc agcgacgcca
    37321 gcatcaaccg tggcaacaaa cgccgcgccc ccagcatttt cgccagcagc ggcgcgaaca
    37381 aaccgataaa gccgataatc cccaccgcat tcaccagcag cgcactgatg actatcgcca
    37441 gcgacagcgc cgccagacgc gcgagcgaca aggccagccc gagattgcgc gccacgccat
    37501 catcaagccc catcagggtt aacgggcgaa gtagcaataa cgtcaacatc acgccgccca
    37561 gcaactgcgg ccataaacgc tcaacgccgc cccagtcggt ttgcgtcagc gttccggtgc
    37621 tccacaggaa catgctttgc agctggtcgt gatggaagat aaccagtagc tgattaattg
    37681 ccccgcaata aaggctcacc accagccccg cgagaataag cgtaaccggc gaaagccgtt
    37741 tcccccacgc gacgccaaag acgattaagc caacgacaca agcgcccacc agtgcggcaa
    37801 actggctcgc catagcacct gggatcgccc aaagtgtggt gacggtaatt cccagttgcg
    37861 cgcctgtagc aacgccaagc gtcgtcggct ccgccagcgg gttacgcagc acttgctgaa
    37921 acagcacgcc caccagcccc aggcccgcgc ccaccagcag cgaaatcgcc agacgcggca
    37981 acaagctgta gtgaaaaatc atctgctcga tgacatcgat gtctggcgac caggcagcct
    38041 gcgcccactg gctacgcggc agcgcctgcg agaagttcat ccaggtgagc gccgtagcga
    38101 caatcactaa cagcgccagc aataacgccg gaaaaagcgc aattcgttta ctcacgcttt
    38161 acctccgatg gcgttatcca gaacgcgcac aaagtgcatt gccgagagtg tcgcaccata
    38221 aaaccagact gcaggtacgc gctgaaagcg tccggcgcgg acaaacggca tggcctgcca
    38281 cagcggggtt gccattagcg catccatgtc tttgctgttg tcgtgatcaa aacagagcac
    38341 atcaacgtct ttatacgctg ccagacgatc gatactgacg gcggtactgc cccagaagtt
    38401 ggtttcccct tgccaggcat ttgggatgcc gtactcatcg agaatttcct ggaacaagct
    38461 gtttggaccg aagaccagca tatggcgcgg atcgataagc gtcgtcagca gtaacgggcg
    38521 tgcaccacgc ttcacaaagc ggggtttcat gctgcggata aagtcttcgt attgcgctaa
    38581 atgcgtttcc gctgcgcttt gcaggttaag taaatctgcc atttccgtca gcgatttacg
    38641 cgccatcgcc aacggctgtt tgccgtcact gaagttaaat ccgcgacccg gcgcaatacg
    38701 tgccagcatt tctgatgaag ggccatatcc tgccgaccag accataaacg acggtttcat
    38761 ttcggtcagc agttcaaggt taggttctgt acgcaaaccg acgtcaatca ctgagtccgg
    38821 caatggtggt tcgctgaccc acaggcgata gttgatggta tccgccacgc cgtaaggcac
    38881 gatgccgagc gccagcagta attccaccgg caaccactcc agcgccacaa tacgattggg
    38941 gtcaatagcc gccgcgtggg cggtattcat ctgccataac aacggagaaa gcgccatcgc
    39001 cgttaacagt cgacggcgcg aaataagagg taagccgctc atcaataaac aaaactcaca
    39061 ggtgcagcac ccgccggatg cggcaaaata cccatcggga tgccataaat catttcgagg
    39121 gtttcgccgc gcataatttc cgcaggcgtt ccctgagcaa tcatttcacc actgcgcagg
    39181 gcgaccagat aatcacagta gcgtgccgcc atattgatat cgtgcaacac ggcaatgacc
    39241 gtcaggccac gttcctgact taaacggtgc accagcgcca gcacatcaac ctggtgggcg
    39301 atatccagcg ccgaagtcgg ttcgtcgagc aacagacaac ggctatcctg cgccaccagc
    39361 atggcgatcc acgcccgctg acgttcgccg ccagagagac tatcgaccag ccgatgcgcc
    39421 agcggtttta agccaaccag cgagatagct tcctcgacct tttcgcgatc tgccgcacca
    39481 aagcgcccca gcgcgccatg ccacgggtaa cgaccaatcg ccaccagttc acgcacggtc
    39541 atcccttctg ccggaggaag ctgctgcggc aaataagcca ctttgcgggc aaacgctttg
    39601 ctgctccagc tttccagcgg ctgggcatca agaagaatct ccccttctga cggaggctga
    39661 tgacgaccaa gcattttgag cagagtggat ttaccagaac cgttgtgacc aatcagaccg
    39721 gtcactttcc cggcaggaaa ggttaacgac agcggatgca aaagcgtgcg cccgggcaca
    39781 cgaaaggaga tattacgcag tgcaaaagtg gtatcggaat gattcgtgta ttcctgcata
    39841 acagccaact tgtgaaatgg gcacggaaat ccgtgcccca aaagagaaat tagaaacgga
    39901 aggttgcggt tgcaacgacc tgacgttctg cgccccagaa gcagccataa gtctggaagc
    39961 aactggcaac atactcacga tcgagcaaat tgttgacgtt cacagcaagg ctggatcccg
    40021 ccataccaaa gcgtgccaga tcatatttta caacagcatc cattacagct gcgctaccca
    40081 ctttaaaagt gtttgccgga tcaccatagc tggaaccaat aaaacgacca cctgttccca
    40141 atgttaaacc agaaagcggc ccttcattga aggtataatc cccccagaga gatgccatat
    40201 gctccggtac ttgttctgga gttttacctt tgagattggt gtctttggtg tattccgcat
    40261 cggtgtaggt ataagaagcc gtcaagttaa tattggcatt taacgccgct tttgcttcaa
    40321 gttccacacc acgagcgcga atctcacctg ctgggacttg cgctaatggg tttgttgggt
    40381 ctgctgtcag gttatttgtt ttcgtcaatt gatagactgc gcccgtaaca acgaccggca
    40441 tatcattcgg aacatatttt acgccagctt catactgttc acctttcgat ggcttatagg
    40501 aaatgcgcgg gttgctccac agatcgaaag cactcggttc aaaggactgg ctatagctaa
    40561 agtaaggtga aataccatta tcgaataagt aatttacacc accgcgccag gtgaactgat
    40621 gatcattgcg ctcaatataa ccttcagtcg gcgtataaga gttttcacga acagtggtcg
    40681 cttgcttaga ccaatcgtag cgtcccccca gagtgaatac ccatttattc cattccgcct
    40741 gatcctgaac ataaatacct gtttgtttgc tttcattcat ctggtatggc tctgcgctac
    40801 caaatgcaaa gtattcaggg tgatatttgt tataaagatc gatggatggt gcggatccaa
    40861 agctggcatt gatatcatta cgcatccgca tgaagtctac ccctgtcagc aaagtatgct
    40921 cgacgtcacc tgtagcaaac ttactttcaa gttgggtatc aacgctaaag ttttgtagac
    40981 gctcattatc caccactgtc ccgcggttta gggtatgacc atcaccagca atccctgtgc
    41041 cataaacact tttctgtgag gttttcattt cactgaaacg cagattctga cgcacggtga
    41101 aggtgtcatt aaaaccatgt tcaaaactat atcccaccat tttctggtta cgggagtatg
    41161 tattattcga tgcaccttca ttgaagtcag tcggtagacg cttaccatta ggcaatggct
    41221 caacggtccc ctctttcggc aaccaaccgt aataacccgt ttctggttca ttctggaaat
    41281 aggacaggaa agtaaaattg gttttgtcgt ctggacgcca tgagaatgat ggagcaatgg
    41341 tataacgctg agattcagag tttttctgct gttcgtttgt tgagcgagcg aggcccgtca
    41401 aacggtaaga gaactcaccg ttatcatcca gtgcatcact gaaatcgaat ccggtctgaa
    41461 acagattatc cgtccccatt ttaaactgaa tttctttcag gggttcagtg gtcggacgct
    41521 tactcaccat agaaataatc ccaccaggat tactttttcc atagagaaca gacgtcggcc
    41581 cacgcatcag ttcaaccctc tcgagcatgt aaggatcaat cacagcatcg ttataaaagt
    41641 tgccctgaag ttttagtcca tcaaggtaat tattttgatt cagaccaaca gatgagaaac
    41701 cacgaatgat aacaaaatca tatgtattag aagcaccacg actgctaacc gtaacgccag
    41761 gtgtataacc taatgcttct tttacagact gaaattgacg catctgcatc tcttcatttg
    41821 tcacaaccga aatagactgc ggcgtttttt caattggtgt atccgtttta gtcgcagtag
    41881 cagagtgctt cgccgcaata gttgcagcag gcccccatgc gctttcttgc ggcgcaggtg
    41941 cagcggtaac ggtgatagtg tcttctttcg gttcaaccgc tgcctgtgca taaacagaca
    42001 tgccgctaac cgctgtggct actacaactg cgattttacg cagtgagtgt tttggctgag
    42061 cagttttgga acgcgccatt ggtatatctc tgatgtaaag tgaatgataa cgtaaacgag
    42121 aatgattatt ataaagataa gataatattc gaaacgctgg cgggttcgca agcggtacgc
    42181 aatctgagcg ttcctgaagg gttaataaac aaccagatga aaagaaaggg ttaatacttt
    42241 aggtgggatg ttattttacc ggatggcaat tcgccatccg gtatttcacg cttagatgtt
    42301 aattactacc aaacatatcc ttgatccaac cggctacacc gtcgctgtct ttctgctctt
    42361 gctgagcagg ttgctgttgc ggctgttgct gcggctgaga agactgatca aacggattgc
    42421 ctgacggctg ctgctgcatc tcgctctgct ggcacagcga ttgcggatcg ctggtccaga
    42481 ccggcaagac acgcatgcca ccgctgcaaa caaagttgcc gtcgtagtcc acgcccatat
    42541 ctgcaatatc ttctggcgga acaagattca gcggcgttgg cgtctggtta gccagataac
    42601 gctgataaat agacattgcc ccgctggcac catacagttt ggtcggctgg ttgttatcac
    42661 ggcctaccca ggtgatggtc accgtgctgc cgtcaatgcc cgcaaaccag gtatctacgt
    42721 tattgttggt agtccctgtt ttccctgcca gatgcaggtt cgggtatttc gccccaagtt
    42781 gacgacccgt accacgttgt accacctgct gcatggtcca tagtgtcaga tacgccgcct
    42841 gcgccggaac agcgcgttct gcctgcggga agctctgata cagcaccttg ccatcttccg
    42901 cgattaccga acgcagtgca gaaagcggtg cacggttacc gccgctggcg atggtctgga
    42961 atgcctgcgc cacttcgatt ggcgttaagt tcaacgcccc cagcagcatc gccggaaccg
    43021 gatgcagctg atctttcggc acgcccagtt tgatccaggt ctccgtaact gcaggcagcc
    43081 ccagcgccat ccccagattt accgtcggca cgttcatcga acgggtcaac gcatccacca
    43141 gcatcactct gccgctttcg ctataacgac ggtcatcgtt ctgcggtgac cagacctggc
    43201 cattcggctg acgcagcgcg attggcgcat cggcaatcca cgtgttcagg cgatagattt
    43261 tcggctggct taaagccgtc agataagtcg ctggttttgc aagggaacca atcgaacgac
    43321 gcgcctgcat cgcacggttg tagcccgcaa actgcggctc agaacctccc accatcgcac
    43381 gaacttcacc actaaagcgg tcgacgacca caattgcagt ttcaagatcg ctcaacttac
    43441 gctgtttctt cagtgccgga atgccttcca cggcggcttt ttctgccgcg tcctgggcca
    43501 ccgagtcaaa ggtagtgaag atcttcacgc cggagagatc ttttacctta tcgcctaatt
    43561 tcgcctgcag ctcctgacgc accagttgca taaaggctgg ctgtggagag attaccccac
    43621 cgcgcggctg tacgcccaga ggacgcgcgc tgagcatttc atacagttca ccatcgataa
    43681 tctgctgttg ttgtagcaaa cgcagcacca gattacgtcg ctccagcgcc agtttcgggt
    43741 tacgccacgg gttgtagatg gacgcccctt tcaccatacc gactaacagc gcctgctggt
    43801 cgaggctgag ctcttctacc gggcgaccaa agtaatacaa gctcgcaagc gggaagccgc
    43861 ggatttcgtt gtcgccgctc tgaccgagat acacctcgtt catatacagc tcaagaatac
    43921 ggtctttgct gtaacgcgcg tccatgatca gcgccatgta agcttcgttc gctttacgcc
    43981 agtaagaacg ctcgctggag aggaacaggt ttttcaccag ctgttgcgtc agcgtactcg
    44041 ctccctgcac cgtgcgtccg gcggtcaggt ttgccagcac cgcacgtccg atggagtaga
    44101 gactgatccc atcatgctcg tagaaatgac ggtcttctgt cgccagcaaa gtatccacca
    44161 gcaagtccgg gaaaccactg cgcggcacaa acagacgctg ctcaccgttt ggcgaagaga
    44221 tcatggtgat caggcgcgga tcaagacgga agaaaccgaa ctgacggttg ttctccatat
    44281 tgacgatcgt cgccagatga tcgccatcaa aggtcagacg cgcacgcacc tgcccttctt
    44341 tgctgtccgg gaaatcaaac ggacggcgga tcatctcaat gctgttggcc tgcacggtaa
    44401 attcgccagg acgggtcatt ttcgacacct gacgatactg tgttgcctcc agcagcttca
    44461 ccatttcgtt cttgctgatg gtcatatctg gttcaaggtt gaccattcgg ccataaactg
    44521 ccgcgggcag ttgccagacc ttgccatcaa tacggctacg aattttttga tcgagataaa
    44581 cgccgtaaat ggcgatcagc acggcaaaaa cgatagccag ttttaacagc agccatagcc
    44641 agccgcgttt gccacgaggc ttacgccctt tgcctttgcc cttacctttg cgcggcatcg
    44701 gttcttcatc ctcatagtca tcataatcgt cgtaatcatc gtcatcttcg taacgacgac
    44761 ggcttacctt ttgtttgacc ggacgcgtcg gtttcccttt gcgtccaatt ggctcgcggt
    44821 cattcccggc catgcttttt ctccgcaata ttcaggcgca aaagcccgat tttctgttct
    44881 tctgttacaa gacagaagaa gatatctctc aaaatttgtg ctcgatcggt cccctctccc
    44941 ttgagggaga gggttagggt gagggtgaac gtgcgcgctg atgccctcac cccggccctc
    45001 tcccacaggg agagggagaa aaccaacctt acgaatactt tttcgtccgt cgcgtcggtg
    45061 ccgtgtttgc cgggtcgtcc ggccagacgt gtttgggata acgccctttc atctcttttt
    45121 gcacctcacg gtacgctcct ttccagaagg cgctcaaatc tcgtgtgatt tgtagtggcc
    45181 tttgggcggg tgaaagcaac tccagcacca gcggcacgcc cccctgggcg atagtcggat
    45241 tagtggcctc accaaacatc tcctgcattc tcaccgccag tgcgggcggg ttatcttcat
    45301 gataacgaat ggcgatccgg cttcccgtcg gcacagtgta atgcgcaggc aattcactat
    45361 ccagacgttg ctgcattccc caatcaagta atccgcgtag tgcctgataa atgtccagtg
    45421 atttcaggcc gcgtagtgaa tgtacgcctg tcatatgtgg cagcagccac gtttccagcg
    45481 ctgccaataa actttcatca tcaaccgctg gccagtcata ttccggcaac cactttgcgg
    45541 cgcataacaa acgcaagcgt agctgttccg cttccgccgt ccagttgagc acgcttaaac
    45601 ctttatcacg gatgccatta agcatcgcct gatgcaactc gtcttctgac ggtttcgcca
    45661 gcggctgcac tttcaccgtc aactgaccga tttgtagccg acgccaggct ttcagcgtac
    45721 cttgcgcgtc atcccattcc acggtgtcag attgctgcac cagctgcgga cagcgttgta
    45781 ctaattcatc aatatcgacc ggtagcgcca gtaaaatccg cgcatccggc gaggcgctgc
    45841 cctgcaataa taacggtgcg atcaaccatt cgtggcggct tagcgcgtcg tcggcatcga
    45901 gcatcgctcc catgccgttt gccagttgat agcgtccatc ttgcccacgg cgatgagcaa
    45961 tgcgatcggc aaacgccccg gcaagtagcg gcgcgataag cgaactgtct gcctcaccgc
    46021 cgcgtacgtt taagcgtttt aacagttgct gactacgttg ctgccaggct ggttgattgc
    46081 gcgaaaacgc cacgcccagg tcactattgc ccatcagtgg cggctcttcg agaatggcgg
    46141 caatttttgc cgcggtagca gcttcgtcgt cgttcttcgc gctaaccagc attgccgcta
    46201 aacgcggatc gttacccagc gctgccattt tttgcccttg cgcactaagc cgttcaccgt
    46261 ccagcgcccc cagcatttgt aacagacgtt tcgcggccat tagattcact actggcggtt
    46321 gatccagcca gctcatctgc gccggatcgc tgcatcccca ttgcagtaat tccatcagca
    46381 aaccggaaag atcgctttgt aagatctccg gttcactttg cgcggcagcg cgttctgctt
    46441 gttctttggc gattaaatgc aggcagatac ccggctccag acgcccggcg cgcccggcac
    46501 gctgcgtcat ggatgcctgg ctaacgcgtt gagtaatcag tcgcgtaagc cccgtgcgcg
    46561 gctcaaaacg cgccacacgc tcctgggcac aatccaccac cagacgaata ccttcaatgg
    46621 ttaaactggt ttcagcaata ttggtcgcca gcaccacttt gcgcatccct tgcggtgccg
    46681 ggaggatcgc ttttcgctga tcgttcagcg acaacgcgcc atacagcggg cagagcaaca
    46741 catcactgcc gatgcgcgaa gccagttgtt cctgcacacg ctgaatttct ccgacgccag
    46801 gtaaaaataa cagtaatgat ccgctttcct gacgcagcat ttcggcggtg gctaccgcaa
    46861 cggcttcgtc aaaacgctga tgcgcgggca gcggtaaata atggcgttca accggaaacg
    46921 agcgcccttc tgagatgacg acgggcgctt ctggcagcat ttgctgcaag cggtcgttat
    46981 ccagcgtagc cgacataatc agcagtttaa ggtcatcacg cagaccttgt tgcacatcga
    47041 gtaacagcgc caacgccaaa tccgcctgca agctgcgctc atgaaattca tcaaggatca
    47101 ccaatccaac accgctcaac tccgggtcac gctggatcat ccgcgtcagc acgccttcgg
    47161 taactacttc cagacgggta tttggcccga cgcagttttg cgcacgcatc cggtagccaa
    47221 cggtatcgcc tggcttttcg ttaagcagct ccgccagccg ttgcgcgacg ttacgcgccg
    47281 ccagacgacg cggctccagc aggataattt tcccgttaat gccgggatgc gccagcagtt
    47341 gcagcggcag ccaggttgat ttcccggccc cggtcggcgc acttaataat acctgcggcg
    47401 cacaatcgag agcggtaagt aattcaggta agacggcagc aacgggcaac gacgacacaa
    47461 aacgctccag agggttaaca ttcttcgcgc cacattgtag catcgcggta attcataacc
    47521 gagtgcctca catgtctgaa ccgcaacgtc tgttctttgc tatcgactta cctgcagaaa
    47581 tccgcgaaca gattatccac tggcgcgcca cacacttccc acctgaggcg ggacgtccgg
    47641 tcgccgccga taatttgcat ctgactctgg catttttagg cgaagtgagc gccgagaaag
    47701 agaaggcgct ttctctttta gccggacgga ttcgtcaacc tggtttcaca ctcacgcttg
    47761 atgacgccgg acaatggctg cgttcgcgtg tggtgtggtt agggatgcgt cagccgccac
    47821 gcggcttaat ccagctggcg aatatgctcc gttcacaggc tgcccgcagc ggttgttttc
    47881 aaagcaatcg tccgtttcat ccacatatta ccttattgcg cgacgccagc gaggcggtga
    47941 caatcccgcc gccaggtttt aactggtctt atgcggtgac ggagttcacc ctttacgcct
    48001 cctcgtttgc ccgtggacgc acacgctaca cgccgctaaa acgctgggcg ctaacgcaat
    48061 aacaaggatt gtcgcaatgg aattctctcc ccctctacag cgcgcgacgc taattcagcg
    48121 ttacaaacgt tttttagccg atgtgatcac acccgatggt cgcgaattaa cgctacactg
    48181 cccgaatacg ggtgcgatga ccggttgtgc aacgcgtggc gataccgtct ggtattcgac
    48241 ttcagacaac accaaacgga aatacccaca cacctgggaa ctaactcaaa gccagagcgg
    48301 cgcatttatt tgcgtcaata cgctttgggc taacaggttg acgaaagagg ctatccttaa
    48361 tgaatcaatt tcagaactgt caggctatag ctcgctgaaa agcgaagtaa aatacggcgc
    48421 agaacgcagc cgtattgact ttatgttgca ggcggattcg cgtccagact gctatattga
    48481 agtgaaatcg gttacgttag cggagaacga acagggatat tttcccgatg cggtcactga
    48541 acgaggtcag aaacaccttc gggagttgat gaacgtagcg gctgaaggcc agcgtgcggt
    48601 tatctttttc gccgtgctgc attcagccat tacacggttt tcacccgcgc gccacatcga
    48661 tgagaaatac gcgcaactat tgtcagaagc tcaacagagg ggggtagaaa ttctggctta
    48721 caaagcggaa atttctgctg aaggcatggc tcttaaaaaa tcactgtcgg ttacattgta
    48781 gtaaagtaag taactggtta atttacattc tggtcgcgtg cgcaaatacg cttttcctca
    48841 cacagttgtc aagtgttacg tttagataat tgctatccgg aaaagcatct gctatttata
    48901 gcggcctcat ttttcccccg aacatgggga tcgatagtgc gtgttaagga gaagcaacat
    48961 gcaagaaggg caaaaccgta aaacatcgtc cctgagtatt ctcgccatcg ctggggtgga
    49021 accatatcag gagaagccgg gcgaagagta tatgaatgaa gcccagctgg cgcacttccg
    49081 tcgtattctg gaagcatggc gtaatcaact cagggatgaa gtcgatcgca ccgttacaca
    49141 tatgcaggat gaagcagcca acttcccgga cccggtagac cgtgcagccc aggaagaaga
    49201 gttcagcctc gaactgcgta accgcgatcg cgagcgtaag ctgatcaaaa agatcgagaa
    49261 gacgctgaaa aaagtggaag acgaagattt cggctactgc gaatcctgcg gtgttgaaat
    49321 tggtattcgc cgtctggaag cgcgcccgac agccgatctg tgcatcgact gcaaaacgct
    49381 ggctgaaatt cgcgaaaaac agatggctgg ctaattacag ccgttccatc acgtttacca
    49441 caggcgggaa atgctcccgc cttatttttt gttcaaagag atgacagaca cacagtatat
    49501 tggccgcttc gccccctctc cttccggcga gcttcatttt ggctctctga tcgccgcgct
    49561 cggcagctat ttgcaggctc gcgcccggca aggtcgctgg ctggtacgca tagaagatat
    49621 cgacccgcct cgtgaagttc ccggtgccgc agaaactatc ctgcgccagc tggaacatta
    49681 cggtctgcac tgggacggcg atgttctctg gcaatcgcaa cgtcacgacg cctatcgtga
    49741 agcactcgcc tggttacatg aacaaggact aagttattac tgcacctgta cgcgtgcgcg
    49801 tattcaaagc attggcggta tttacgacgg tcattgccgg gtgttgcatc atggaccaga
    49861 caatgccgca gtgcgtatcc gccagcagca tccggtcacg caatttactg accagctgcg
    49921 cggcattatt cacgccgacg aaaaactggc acgggaagat tttatcattc atcgccgtga
    49981 tgggttgttc gcctacaacc tggctgttgt ggttgatgat catttccagg gcgttacaga
    50041 aatagtgcgt ggggctgatc tgattgaacc aacagtaagg caaatctcgc tgtaccagct
    50101 ttttggctgg aaagtgccag attacattca tctgccgctg gcgcttaatc cacaaggcgc
    50161 taaactttcc aagcagaatc atgcgcctgc gttgccgaaa ggcgatccac gtccggtact
    50221 aatcgcggca cttcaatttc tgggtcagca ggcagaagca cactggcagg atttcagcgt
    50281 cgaacaaatc cttcagtcag cagtcaaaaa ctggacgctg accgccgtgc ccgagtcggc
    50341 aattgtaaat tcaacattct caaatgcgtc atgctgagct atgattagcc gctatttttt
    50401 tgtcctgaat gatgtttgac actaccgagg tgtactattt ttacccgagt cgctaatttt
    50461 tgccgcaagg tgctaagccg cgaggaaagc gaggatgaac aggcagtcgc ccgtccacag
    50521 gtgacggtga tcccgcgtga gcagcatgct atttcccgca aagatatcag tgaaaatgcc
    50581 ctgaaggtaa tgtacaggct caacaaagcg ggatacgaag cctggctggt tggcggcggc
    50641 gtgcgcgacc tgttacttgg caaaaagccg aaagattttg acgtaaccac taacgccacg
    50701 cctgagcagg tgcgcaaact gttccgtaac tgccgcctgg tgggtcgccg tttccgtctg
    50761 gctcatgtaa tgtttggccc ggagattatc gaagttgcga ccttccgtgg acaccacgaa
    50821 ggtaacgtca gcgaccgcac gacctcccaa cgcgggcaaa acggcatgtt gctgcgcgac
    50881 aacattttcg gctccatcga agaagacgcc cagcgccgcg atttcactat caacagcctc
    50941 tattacagcg tagcggattt taccgtccgt gattacgttg gcggtatgaa ggatctgaag
    51001 gacggcgtta tccgtctgat tggtaacccg gaaacgcgct accgtgaaga tccggtacgg
    51061 atgctgcgcg cggtacgttt tgccgccaaa ttgggtatgc gcatcagccc ggaaaccgca
    51121 gaaccgatcc ctcgcctcgc taccctgctg aacgatatcc caccggcacg cctgtttgaa
    51181 gaatcgctta aactgctaca agcgggctac ggttacgaaa cctataagct gttgtgtgaa
    51241 tatcatctgt tccagccgct gttcccgacc attacccgct acttcacgga aaatggcgac
    51301 agcccgatgg agcggatcat tgaacaggtg ctgaagaata ccgatacgcg tatccataac
    51361 gatatgcgcg tgaacccggc gttcttgttt gccgccatgt tctggtaccc actgctggag
    51421 acggcacaga agatcgccca ggaaagcggc ctgacctatc acgacgcttt cgcgctggcg
    51481 atgaacgacg tgctggacga agcctgccgt tcactggcaa tcccgaaacg cctgacgacg
    51541 ttaacccgcg atatctggca gttgcagttg cgtatgtccc gacgtcaggg taaacgcaca
    51601 tggaaactgc tggagcatcc taagttccgt gcggcttatg acctgttggc cttgcgagct
    51661 gaagttgaac gtaacgctga actgcagcgt ctggtgaaat ggtggggtga gttccaggtt
    51721 tccgcgccac cagatcaaaa agggatgctt aacgagttgg atgaagagcc gtcaccgcgt
    51781 cgccgtactc gtcgtccacg caaacgcgca ccgcgtcgtg agggtaccgc atgacagtgg
    51841 cgtatattgc cataggcagc aatctggcct ctccgctgga gcaggtcaat gctgccctga
    51901 aagcattagg cgatatccct gaaagccaca ttcttgccgt ttcttcgttt taccgcactc
    51961 caccgctggg gccgcaagat caacccgatt acctcaacgc cgccgtggcg cttgaaactt
    52021 cgctcgcccc ggaagagtta ctcaaccata cacagcgcat agaactgcaa cagggtcgtg
    52081 ttcgtaaagc tgaacgctgg ggaccacgca cgctggatct cgatatcatg ctgtttggta
    52141 atgaagtgat aaataccgaa cgcctgaccg ttccgcacta cgatatgaag aatcgtggat
    52201 ttatgctgtg gccgctgttt gagattgcac cggagctggc gtttcctgat ggggagacgt
    52261 tgcgtgaagt cttacataca agagcatttg acgaattaag caaatggtaa tttatttata
    52321 ttacgaatat aaatattttc tgaaaatcat acatcctctc tttccttaat aaaaatttta
    52381 gtttaaacat ttttgcagaa acataatata aattcccgca gataaatatc aatcccatcg
    52441 ccatacccta accaattgca cttgtgaatt tatttttgct taattttgaa ttaagaaaat
    52501 ttaatttgtt caaaagccct taaagtgcac aataggcgtc ctaatgaaca atggttgtaa
    52561 attttggtaa caaatatcat tgctcattaa tatctagcat ctgcttataa catttgtgtc
    52621 atgaatagtc attcttcaaa ccagtagtct gactaatgga attttattca aggattttta
    52681 tgtctaaaaa attaggtttt gccctgtctg gtcttatgct ggcaatggtt gcaggtactg
    52741 cttctgctga tatggatggt ggtcagttaa atatcagtgg tctggttgtt gataacacct
    52801 gtgaaacccg cgttgacggc ggtaataaag atggtctgat cctgctgcaa actgcaactg
    52861 ttggtgaaat cgatgctggc gtgctgaatg acaccgttgg cgctaaagcc aaacctttca
    52921 gcatcaccgt tgactgcagc aaagcaaatc cgaatccagg tagtacagct aaaatgacct
    52981 tcggttctgt tttctttggt aatagcaaag gaacgctgaa caacgacatg tctattaaca
    53041 acccgtctga cggcgtgaat atcgctctgc acaatattga tggttccact atcaaacagg
    53101 ttcaaattaa caaccctggc gatgtgtata ctaaagccct ggatgcaacg acaaaatctg
    53161 ctgtttatga ttttaaagcg tcttacgttc gtgccgtcgc agaccaaaca gcaactgctg
    53221 gttatgtaaa aactaacact gcatacacca ttacttatca gtaagtattc accaattaaa
    53281 ttgattgaat gaatatacag ggaatagtgt taataatttc tatttttata ttattccctg
    53341 ttttaattaa ctctgtcagg gatggtttat gttttttaac accaaacata caacagcttt
    53401 atgcttcgta acctgtatgg cttttagttc atcgtctatt gcggacattg tcatttcggg
    53461 tactcgcgta atatataaaa gcgatcaaaa aagtgtcaac gtacgtctgg aaaataaagg
    53521 gaataacccg ttgcttgtcc agagttggtt agatactggc gataacaacg ctgagcctgg
    53581 cagtattaca gtccctttta ctgccacgcc gccagtatcg cgtattgatg ccaaacgtgg
    53641 gcaaacaatc aaattaatgt acacagccag cacctcactg cctaaagaca gagagagcgt
    53701 gttctggttt aacgtactgg aagttccacc aaaaccagat gcagaaaagg tcgcgaatca
    53761 aagtctgctg caactggcat ttcgcacacg tataaaactt ttctatcgcc cggatggatt
    53821 gaagggaaat ccctctgaag ccccgttagc cctgaagtgg ttctggtcag gttcagaagg
    53881 taaggcgtca ttgcgagtga ccaatccaac cccttactac gtctctttta gcagtggtga
    53941 tttagaagct agcggtaaac gctatccgat tgatgtgaaa atgattgcac catttagtga
    54001 tgaggtcatg aaagtcactg gccttaatgg caaagcaagt tctgcaaaag tacattttta
    54061 cgccattaat gattttggtg gcgccattga aggtaatgcc agtctgtaat caggcaagga
    54121 tataattccg caggaagcat aacgtgacta taaaaccaac taaaaattat cataatcatc
    54181 tgacccgtat cgctacgttt tgcgcgctgc tgtattgcaa tagtgctttc tgtgctgaac
    54241 ttgttgaata tgaccatacc ttcctgatgg ggcagaatgc ctcaaatatt gatctcagcc
    54301 ggtatagtga aggtaaccct gctataccgg gtatgtacga cgtcagtgtt tatgtcaacg
    54361 atcaacctat tattaaccaa agcattacat ttattgaaat tgaaggaaaa aaaacgcaca
    54421 agcctgtatc acacttaaga atttattgca gtttcatata aattctcccg atataaataa
    54481 cgaaaaagcc gttctgcttg ccagggatga aacactcggc aattgcctta atttgacgga
    54541 aattatccct caggcttctg ttcgttatga cgttaacgag caacgtctgg atatagacgt
    54601 tcctcaggca tgggtaatga aaaattacca aaactatgtt gatccatcgt tatgggaaaa
    54661 tggcattaat gcggccatgt tgtcatacaa cctcaacgga tatcatagtg aaactcctgg
    54721 tcgaagaaat gacagcattt atgctgcttt taacggtggg atgaatttag gtgcatggcg
    54781 actgcgtgcc tcaggcaact acaactggat gaccgattct ggcagtaatt atgattttaa
    54841 gaatcggtat atacagcgtg atatcgcctc gctgcgttct caactcattc ttggtgagtc
    54901 ttatacaacg ggcgaaacct ttgattctgt cagtatccgc ggcattcgtt tatacagcga
    54961 cagccgcatg ttgcctccga cattagcgag ttttgcgcct atcattcatg gcgttgctaa
    55021 taccaacgcc aaagtcacta ttacgcaagg tggctataag atttatgaaa cgactgtgcc
    55081 gccaggcgct ttcgtcattg atgatctgag tccgtcaggt tacggcagcg atcttattgt
    55141 tactgtcgaa gaatccgatg gctcaaagcg gacattctcg caaccttttt catcggtcgt
    55201 tcaaatgtta cgccctggcg ttggacgttg ggatattagc ggcggccagg tcttaaaaga
    55261 tgatattcag gatgagccta atttatttca ggccagctac tactacggtc tgaataacta
    55321 tctaacg
//
LOCUS       DAKISA010000015        52919 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.015, whole genome shotgun sequence.
ACCESSION   DAKISA010000015 DAKISA010000000
VERSION     DAKISA010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52919
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.015"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..135)
                     /gene="tabA"
                     /locus_tag="OVW45_000951"
     CDS             complement(<1..135)
                     /gene="tabA"
                     /locus_tag="OVW45_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCV5525464.1"
                     /translation="MIIGNIHNLQPWLPQELRLAIEHIKAHVTAETPKGKHDIEGNRL
                     F"
     gene            complement(280..873)
                     /locus_tag="OVW45_000952"
     CDS             complement(280..873)
                     /locus_tag="OVW45_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCV5525465.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            944..1657
                     /gene="bdcA"
                     /locus_tag="OVW45_000953"
     CDS             944..1657
                     /gene="bdcA"
                     /locus_tag="OVW45_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCV5525466.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQKTGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMAREL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            1788..2183
                     /locus_tag="OVW45_000954"
     CDS             1788..2183
                     /locus_tag="OVW45_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCV5525467.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            2464..2598
                     /gene="pyrL"
                     /locus_tag="OVW45_000955"
     CDS             2464..2598
                     /gene="pyrL"
                     /locus_tag="OVW45_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCV5525468.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            2602..3537
                     /gene="pyrB"
                     /locus_tag="OVW45_000956"
     CDS             2602..3537
                     /gene="pyrB"
                     /locus_tag="OVW45_000956"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCV5525469.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            3550..4011
                     /gene="pyrI"
                     /locus_tag="OVW45_000957"
     CDS             3550..4011
                     /gene="pyrI"
                     /locus_tag="OVW45_000957"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCV5525470.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            4084..4470
                     /gene="ridA"
                     /locus_tag="OVW45_000958"
     CDS             4084..4470
                     /gene="ridA"
                     /locus_tag="OVW45_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCV5525471.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="OVW45_000959"
     CDS             complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="OVW45_000959"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCV5525472.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="OVW45_000960"
     CDS             complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="OVW45_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCV5525473.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            7751..8698
                     /gene="treR"
                     /locus_tag="OVW45_000961"
     CDS             7751..8698
                     /gene="treR"
                     /locus_tag="OVW45_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCV5525474.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            8817..10238
                     /gene="treB"
                     /locus_tag="OVW45_000962"
     CDS             8817..10238
                     /gene="treB"
                     /locus_tag="OVW45_000962"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCV5525475.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            10288..11943
                     /gene="treC"
                     /locus_tag="OVW45_000963"
     CDS             10288..11943
                     /gene="treC"
                     /locus_tag="OVW45_000963"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCV5525476.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            12337..14475
                     /gene="nrdD"
                     /locus_tag="OVW45_000964"
     CDS             12337..14475
                     /gene="nrdD"
                     /locus_tag="OVW45_000964"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCV5525477.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSRESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            14633..15097
                     /gene="nrdG"
                     /locus_tag="OVW45_000965"
     CDS             14633..15097
                     /gene="nrdG"
                     /locus_tag="OVW45_000965"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCV5525478.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="OVW45_000966"
     CDS             complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="OVW45_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HCV5525479.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     ATQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="OVW45_000967"
     CDS             complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="OVW45_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCV5525480.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGLADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLLPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            17158..17709
                     /locus_tag="OVW45_000968"
     CDS             17158..17709
                     /locus_tag="OVW45_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCV5525481.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="OVW45_000969"
     CDS             complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="OVW45_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCV5525482.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVASADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            19341..20339
                     /gene="fbp"
                     /locus_tag="OVW45_000970"
     CDS             19341..20339
                     /gene="fbp"
                     /locus_tag="OVW45_000970"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCV5525483.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="OVW45_000971"
     CDS             complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="OVW45_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HCV5525484.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(21354..22376)
                     /locus_tag="OVW45_000972"
     CDS             complement(21354..22376)
                     /locus_tag="OVW45_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCV5525485.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSGVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(22390..23892)
                     /locus_tag="OVW45_000973"
     CDS             complement(22390..23892)
                     /locus_tag="OVW45_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCV5525486.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFFLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHVLQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="OVW45_000974"
     CDS             complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="OVW45_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HCV5525487.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTGGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            25298..25828
                     /gene="ppa"
                     /locus_tag="OVW45_000975"
     CDS             25298..25828
                     /gene="ppa"
                     /locus_tag="OVW45_000975"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCV5525488.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(26207..26548)
                     /locus_tag="OVW45_000976"
     CDS             complement(26207..26548)
                     /locus_tag="OVW45_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCV5525489.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="OVW45_000977"
     CDS             complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="OVW45_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCV5525490.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGNVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDIILNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="OVW45_000978"
     CDS             complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="OVW45_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCV5525491.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPLKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSDWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGGGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            32266..32904
                     /gene="msrA"
                     /locus_tag="OVW45_000979"
     CDS             32266..32904
                     /gene="msrA"
                     /locus_tag="OVW45_000979"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCV5525492.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            33227..34570
                     /locus_tag="OVW45_000980"
     CDS             33227..34570
                     /locus_tag="OVW45_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCV5525493.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(34650..34856)
                     /locus_tag="OVW45_000981"
     CDS             complement(34650..34856)
                     /locus_tag="OVW45_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCV5525494.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            35181..35735
                     /locus_tag="OVW45_000982"
     CDS             35181..35735
                     /locus_tag="OVW45_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCV5525495.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            complement(35825..36736)
                     /locus_tag="OVW45_000983"
     CDS             complement(35825..36736)
                     /locus_tag="OVW45_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525496.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSYTKTHSNNFFNNIQHAFKKKDWI
                     SNYDSLLTLREFFRCATQIDKNSYQVLSSKNGTVNAMDKFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIHQ
                     AIGDVSLGYALLEDIVSKLKRGKFELQIPGGGIKECDGWYIHEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPV"
     gene            complement(36948..37688)
                     /gene="cysQ"
                     /locus_tag="OVW45_000984"
     CDS             complement(36948..37688)
                     /gene="cysQ"
                     /locus_tag="OVW45_000984"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCV5525497.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            37878..39821
                     /gene="cpdB"
                     /locus_tag="OVW45_000985"
     CDS             37878..39821
                     /gene="cpdB"
                     /locus_tag="OVW45_000985"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCV5525498.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKTGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINTNAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(39939..40319)
                     /locus_tag="OVW45_000986"
     CDS             complement(39939..40319)
                     /locus_tag="OVW45_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCV5525499.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            40408..41268
                     /gene="qorB"
                     /locus_tag="OVW45_000987"
     CDS             40408..41268
                     /gene="qorB"
                     /locus_tag="OVW45_000987"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCV5525500.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            41376..42341
                     /locus_tag="OVW45_000988"
     CDS             41376..42341
                     /locus_tag="OVW45_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCV5525501.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            42449..43111
                     /gene="ytfE"
                     /locus_tag="OVW45_000989"
     CDS             42449..43111
                     /gene="ytfE"
                     /locus_tag="OVW45_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCV5525502.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(43156..44568)
                     /gene="cycA"
                     /locus_tag="OVW45_000990"
                     /gene_synonym="dagA"
     CDS             complement(43156..44568)
                     /gene="cycA"
                     /locus_tag="OVW45_000990"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCV5525503.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(44877..45497)
                     /gene="fklB"
                     /locus_tag="OVW45_000991"
     CDS             complement(44877..45497)
                     /gene="fklB"
                     /locus_tag="OVW45_000991"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCV5525504.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            45716..46354
                     /gene="ytfB"
                     /locus_tag="OVW45_000992"
     CDS             45716..46354
                     /gene="ytfB"
                     /locus_tag="OVW45_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HCV5525505.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(46338..46985)
                     /locus_tag="OVW45_000993"
     CDS             complement(46338..46985)
                     /locus_tag="OVW45_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCV5525506.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSLRLLEINHAHLQLMESLLDEGKKHNIFKPDIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            47197..47988
                     /locus_tag="OVW45_000994"
     CDS             47197..47988
                     /locus_tag="OVW45_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HCV5525507.1"
                     /translation="MKSTRPVAVITGAARGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLNAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            47998..48840
                     /locus_tag="OVW45_000995"
     CDS             47998..48840
                     /locus_tag="OVW45_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCV5525508.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIHQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            48850..49626
                     /locus_tag="OVW45_000996"
     CDS             48850..49626
                     /locus_tag="OVW45_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HCV5525509.1"
                     /translation="MSDQPVLFSRAAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNLPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQAANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYPHQLLAGLMSATQDCQQATRAFAEKSSVSFHNQ"
     gene            49636..51167
                     /locus_tag="OVW45_000997"
                     /pseudo
     CDS             49636..51167
                     /locus_tag="OVW45_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
     gene            51164..>52919
                     /locus_tag="OVW45_000998"
     CDS             51164..>52919
                     /locus_tag="OVW45_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HCV5525510.1"
                     /translation="MSQLDDTILDALTHVTFPKGFVSAEPTSIVTVEDVDYPIWQVDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFA
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPVLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYTYSVDAEGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQSGRQVFMDFNRNPEAVPGDLPFSLDRLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGSFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLVAPT
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDHAGFICHADKVRRATRDALLLSEFV
                     QRHGLAIKHVGEVVELFMW"
BASE COUNT        12943 a        13980 c        13314 g        12682 t
ORIGIN      
        1 aaacagacga ttgccttcga tatcgtgctt gccctttggc gtttctgccg taacgtgtgc
       61 tttgatatgc tcaatcgcca ggcgtaactc ctggggtagc cacggctgaa gattatgaat
      121 atttccgatg atcatctttt ccactcctta attaaaacaa tgtttcattt ccatcgttat
      181 acgaaaagat gatcaattcc gctagcctta atgcgtgaat tattgtctta tcacaactta
      241 tcggcaggcg cgttcccgcc tttcgggtca aggcccgcat cactccttga gaatagtttt
      301 tatcgcttcc cccgccagtg cagctgttgc acagagttgt tcgatggagt gcccctcccg
      361 agccttcgca gaaagccctg acatcacggt actcataaaa tcagtcacac attgtgcgct
      421 ttgtggatgc cgcctggcga tatagtcata aatggccgtt tctgcggcgt gataatattg
      481 aacggcaata tcacgcgctt gtggatcatg actatgaatg ccttcaagaa ccatacagcc
      541 agcgcagccg ccgttttggc tatatctgcg cgccgcttct tttaatacct cagccaggca
      601 ctcccctacc ggacgatcat cacgaagaat atcggcaagc ggaatagctt ccgtaccgac
      661 atattcattg agtacacggc taaataaccc agccttactg ccaaaagccg cgtagaggct
      721 cggggggtta ataccaagat aatcagtaac ttcagcaaca ctgacagcat cgaaaccttt
      781 ttggtgaaaa agcacttttg ccgcagagac tgcctgctca ggagcgaaac gtctgggacg
      841 acctggaacg cgagattgtt ttttagtgac cattacaaaa cttgttgaca gaaagttaaa
      901 acagttttgt aatgcatgtt acataataaa tcaaggagtc cttatgggcg cttttacagg
      961 taagacagtt ctcatcctcg gtggcagtcg tggtatcggt gccgctatcg tacgtcgttt
     1021 cgtcaccgat ggggccaatg tacgattcac ctatgcgggg tcgaaagatg ccgctgaaca
     1081 cctggcacaa aagactggag cgacagcagt attcacagat agtgctgaca gagacgctgt
     1141 cattgatgtc gttcgtaaga gcggcgcatt ggatatcctg gtggtaaatg caggtattgg
     1201 cgtctttggc gatgccctgg aattgaatgc cgacgatatt gatcgccttt tcaaaatcaa
     1261 tattcatgct ccttatcatg cctctgttga agccgcccgg cagatgcccg aaggcgggcg
     1321 catcttaatc atcggctccg tgaatggcga tcgtatgcct gttgcaggca tggctgctta
     1381 tgccgccagt aaatctgccc tgcaaggcat ggcgcgcgag ctggcccgtg atttcggacc
     1441 gcgtgggatc accatcaacg tcgtccagcc agggccaatt gataccgacg ctaatcccgc
     1501 caacgggcca atgcgcgata tgttgcatag ttttatggct atcaaaagac atgggcaacc
     1561 ggaagaggtc gctggtatgg tcgcatggtt agcagggcca gaagccagtt ttgttaccgg
     1621 cgcgatgcat accattgatg gcgcgtttgg cgcataaccg actacgctca attaagccca
     1681 gccatttccc atgatgtctg ggttttgttt actcacgtcg tccgctaaaa gcggctcctg
     1741 gtaaatataa atcttctgtt catgtcgtaa atcttgagga taaccaaatg gtagaaagaa
     1801 ccgctgtttt ccctgcaggc cgacattcac tgtatgctga acatcgttat tctgcggcta
     1861 ttcgttccgg cgatttgctg tttgtttccg ggcaagtagg aagtcgagag gacggaacac
     1921 cagaacccga ttttcagcaa caagtcagac tggcatttga taatttgcat gcgaccctgg
     1981 ctgctgcggg atgcactttt gacgatatca ttgatgttac gagcttccat accgatccag
     2041 aaaaccaatt tgaagacatc atgacggtga aaaatgaaat atttagcgcc ccaccttatc
     2101 caaactggac ggcggtaggt gttacatggc tggcaggctt tgattttgaa attaaagtga
     2161 tagcgcgcat ccctgagcag taagcaatag tgttagccgt tcgttttcac actccgccct
     2221 ataagtcgga tgaatggaat aaaatgcata tctgattgcg tgaaagtgaa aaaggaaaaa
     2281 gcaggaaatg tctgcaatta ttgataccga acgaccgttc ccctgcagaa tcacaacaaa
     2341 taaaaatgca tgtattttga cttataattc aaataaaccg tttgcgctga caaaatattg
     2401 catcaaatgc ttgcaccgct tctgacgatg agtataatgc cggacaattt gccgggagga
     2461 tgtatggttc agtgtgttcg acattttgtc ttaccgcgtc tgaaaaaaga cgctggcctg
     2521 ccgtttttct tcccgttgat cacccattcc cagcccctca atcgaggggc ttttttttgc
     2581 ccaggcgtca ggagataaaa gatggctaat ccgctatatc agaaacatat catttccata
     2641 aacgacctta gtcgcgatga ccttaatctg gtgctggcga cagcggcgaa actgaaagca
     2701 aacccgcaac cagagctgtt gaagcacaaa gtcattgcca gctgtttctt cgaagcctct
     2761 acccgtaccc gcctctcttt cgaaacttcc atgcaccgcc tgggtgccag cgtggtgggc
     2821 ttctccgaca gcgccaatac atcactgggt aaaaagggcg aaacgctggc cgataccatt
     2881 tcggttatca gcacttacgt cgatgcgata gtgatgcgtc atccgcagga aggtgcggcg
     2941 cgcctggcca ccgagttttc cggcaatgta ccggtactga atgccggtga tggctccaac
     3001 caacatccga cgcaaacctt gctggactta ttcactattc aggaaaccca ggggcgtctg
     3061 gacaatctcc acgtcgcaat ggttggtgac ctgaaatatg gccgcaccgt tcactccctg
     3121 actcaggcgt tagcgaagtt cgacggcaac cgtttttact tcatcgcgcc ggacgcgctg
     3181 gcaatgccgc aatacattct ggatatgctt gatgaaaaag ggatcgcctg gagtctgcac
     3241 agctctattg aagaagtgat ggcggaagta gacatcctgt acatgacccg cgtgcaaaaa
     3301 gagcgtctgg acccgtccga gtacgccaac gtgaaagcgc agtttgttct tcgcgccagc
     3361 gatctccaca acgccaaagc caatatgaaa gtgctgcatc cgctgccgcg tgttgatgag
     3421 attgcgacgg atgttgataa aacgccacac gcctggtact tccagcaggc aggcaacggg
     3481 attttcgctc gccaggcgtt actggcactg gttctgaatc gcgatctggt actgtaaggg
     3541 gaaatagaga tgacacacga taataaattg caggttgaag ctattaaacg cggcacggta
     3601 attgaccata tccccgccca gatcggtttt aagctgttga gtctgttcaa gctgaccgaa
     3661 acggatcagc gcatcaccat tggtctgaac ctgccttctg gcgagatggg ccgcaaagat
     3721 ctgatcaaaa tcgaaaatac ctttttgagt gaagatcagg tagatcaact ggcattgtat
     3781 gcgccgcaag ccacggttaa ccgtatcgac aactatgaag tggtgggtaa atcacgcccc
     3841 agtctgccgg agcgcatcga caatgtgctg gtctgcccga acagcaactg tatcagccat
     3901 gccgaaccgg tttcttccag ctttgccgtg cgaaaacgcg ccaatgacat cgcgctcaaa
     3961 tgcaaatact gtgaaaaaga gttttcccat aatgtggtgc tggccaatta attgcggttg
     4021 gtaataaaag tctggctccc tataatgagc cagacttttt accgctgtaa taaaggagaa
     4081 atcatgagca aaactatcgc gacggaaaat gcaccggcag ctatcggtcc ttacgtacag
     4141 ggcgttgatc tgggcaatat gatcatcacc tccggtcaga tcccggtaaa tccgaaaacg
     4201 ggcgaagtac cggcagacgt cgctgcacag gcacgtcagt cgctggataa cgtaaaagcg
     4261 atcgtcgaag ccgctggcct gaaagtgggc gacatcgtta aaaccaccgt gtttgtgaaa
     4321 gatctgaacg acttcgcaac cgttaatgct acctacgaag ccttcttcac cgaacacaat
     4381 gccaccttcc cggcacgttc ttgcgttgaa gttgcccgtc tgccgaaaga cgtgaaaatt
     4441 gagatcgaag cgatcgctgt tcgtcgctaa tcttgatgga aatccgggct atcatgcccg
     4501 gattaagtct gatgacaaac acaaaatcgc ctgatgcgct acgcttatca ggcctacgtg
     4561 attcctgcaa tttattgaat ttgttggccg gataaggcat ttacgccgca tccggcatga
     4621 acaaaactca ctttgtctgc aatctgaatc caggccacca tgcccagttg attctttatt
     4681 gccagccgta acgacggcta taaaatcctt tcaccagttg ggttaacgtc atataccctg
     4741 ccagaatcgc aaccagccac gggaaatagc ttaacggcag cgcctgcagt tgcagataac
     4801 tggccagcgg tgaaaacggc aatgcgatcc cgacaatcat cacgatcacg gtcatgatca
     4861 ttaacggcca cgatgcacag ctctgaataa acggcacacg gcgggtgcgg atcatatgca
     4921 caatcagcgt ttgcgacagc aagcccacca caaaccatcc cgactggaac agcgtttgcg
     4981 tttccggcgt gttggcatgg aatacccacc acatcaggca aaacgtcaga atatcgaaga
     5041 tcgagctgat cggtccgaag aagatcataa agcgccccag atccgccgga ttccaacgct
     5101 gcggcttttg aatttgctcg tcgtcgacgt tatcaaacgg gatcgccacc tgtgacacat
     5161 catacagcag gttctgaata agcaagtgta acggcaacat cggcaggaag ggcaagaaag
     5221 cactcgccac cagcacgctg aacacattac cgaagttgga gctcgccgtc attttgatgt
     5281 atttcagcat gttggcgaaa gtgcgacgtc cttcaataac cccctcttcc agcaccatca
     5341 ggcttttttc cagcaggatg atatcagccg cttcacgggc aatatctacc gcgccgtcca
     5401 cagaaatgcc gatatccgca gcgcgtaagg cgggcgcatc attaatacca tcgcccataa
     5461 agccaaccac atgcccttcg cgcttcagta aggtcacaat gcgttcttta tgcatcggcg
     5521 tcagacgagc aaacagcgtg gtgcgctgtg cgagatttgc cagttcgtcg tcagatagcg
     5581 tttcaatatc actaccaatg accacctctc ccgcgtccag ccccacttca tggcacactt
     5641 tcgctgcaac taactcactg tcgccagtaa ggatttttac ggtaattcca ctcgctttta
     5701 atgccttcag tgccggagca gttgtctctt ttggcggatc aaggaaagca atatatcctt
     5761 cgaggatcag gtcggattca tccgcccgct ggtaatctcc ttcacgcgct ggcaggtatt
     5821 tcgtcgccac cgcaaccacg cgcagcccct gacgattcag cgtatcagta acccgcttaa
     5881 tcttacgcag catgatgtca tcgagcggca caatctcgcc attgtgacgc acctgcgaac
     5941 acacattgag gatttcctgc aatgcacctt tgcaaaccag ctgatggtgc tcggtatttt
     6001 ctgccactac caccgacatc cggcgacgct cgaaatcaaa cggaatctca tcaatttttt
     6061 gccaacggct ggccagagag cgcgctgact cttcatccgt accttcgagc accgctgtat
     6121 cgagtaggtt tttaagtccg gtctgataat gactgttcaa ccacgcgcta tgcagcacgc
     6181 gttcgctggt tttaccggag atatcggtat gattctccag cacaattttg tcctgtgtca
     6241 gggtgccagt tttatcagtg cacagaatat ccattgcgcc aaagttctga atagcatcca
     6301 gatgtttgac gatcactttc tgtttcgaca gctttactgc gccgcgcgcc agcgtcgagg
     6361 taacaatcat cggcaacatt tccggcgtta agcctaccgc gaccgaaagc gcaaacagtg
     6421 ccgcttccca ccagtcgcct ttggtgtaac cgttgataag caacaccacc ggagccatca
     6481 ccagcataaa gcgaatcagc agcatactga cgcggctgat cccctgctga aaggcattcg
     6541 gctcgctttc ctgctcacta acacgccccg ccagttgacc aaaccaggtg ttggctcctg
     6601 tagcaatcac cattgcttgt gccgtgccgc tcaccacagt ggtgcccata aaacacaggg
     6661 tgtcacactc cagcggattg ctgtgctccg gctggcgagt ggtagcggct ttttccacgg
     6721 gcagagactc accggttaac gacgcctgag cgacgaacag atcccgcgcc tgcaagatac
     6781 gtaaatctgc cgggatcata tcccccgccg ccagttttat aatatcgccg ggcaccagct
     6841 ggtcgatcgg gatctccagc cagccatttt cgcctttgtc gttaattacg cgcagcaccg
     6901 tcgcggtatt gctgaccatc gctttcaggg catctgccgc tttagtggaa cgtgcttcct
     6961 gaataaagtt cagcaacgta gaaatagcga ccattagcgc gataacgccc gcggcaaata
     7021 aatcttcagt ggcgtaagaa atagcgccga gaatggtgag taaaatatta aatgggttgc
     7081 gatagcagac ccataaatgt acccaccacg gcgacggttg ttgtgcgggt aatttatttt
     7141 caccatgttg ttcgcgggca gattccactt ccgcctgatt taacccttcc ggatgggtgt
     7201 cgaacgtttt ccacaattct tcttcgggca tcaccgccat tttcaggcaa tgcgcactta
     7261 aggacggcgg gaccgccgta ttcactgtct gctgcgcgcc aggcaatgga tcgcgatgaa
     7321 ccagacggga aggtaaatgg cgaatgagcc gggtaaaaat ttctttaaac ataaggagtc
     7381 cctccgcact gtctgaaaca gtacagaaat gtcttcaggc gtggcgaagc cttacctgaa
     7441 ggtaataaag ctgagcaggg acgttaaaac gtcacggcct tacgcatccg gtagcgccgt
     7501 gaaaagacgg gcttaccgga aaagttttaa tctccgtcga gggagaggcg tgggatcagg
     7561 ttccatataa cctccggtaa gtgaaaaaag cgccgcaaat aataaggaga atccaataat
     7621 attcgtagca tttatggcga tataatactt gctggcaact aaaccaacga taaaccagac
     7681 tttacccatt gctgaatgca cgggtaaagt taggctcaaa taattaaaca acacgttaca
     7741 ggacaacagg atgcaaaatc ggctgactat caaagacatc gcacgcttaa gcggcgtggg
     7801 gaaatctaca gtttcccggg tgctgaataa cgaaagcggc gtgagccagc gcacccgcga
     7861 gcgtgttgaa gcagtgatga atcagcatgg attttcccct tcccgctctg cgcgcgctat
     7921 gcgtgggcaa agcgataaag tggtcgccat cattgttacc cgtctggatt cgttgtcaga
     7981 aaatctcgcc gttcaaacca tgctgccagc gttctatgaa caaggttacg acccaatcat
     8041 gatggaaagc cagttttccc cgcaattagt tgccgaacat ttgggggtgc tgaaacggcg
     8101 taatatcgac ggcgtagtgc tgttcggttt tactggcata acagaagaaa tgttagccca
     8161 ctggcagtca tcgctggttc tgctggcgcg tgacgcaaaa ggctttgctt cggtctgtta
     8221 tgacgacgaa ggggcaatca agatcctgat gcaacggctg tatgaccagg ggcatcgtaa
     8281 tatcagttat ctcggcgtgc cgcatagtga cgtgacaacc ggtaagcgac gtcacgaagc
     8341 ctacctggcg ttctgcaaag cgcataaact gcatcccgtt gccgctctgc cagggcttgc
     8401 tatgaagcaa ggctatgaga acgtggcaaa agtgattacg cctgaaacta ctgcgttact
     8461 gtgcgcaacc gacacgctgg cacttggcgc aagtaaatac ctgcaagagc aacgcatcga
     8521 caccttgcaa ctggcgagcg tcggtaatac accgttaatg aaattcctcc atccggagat
     8581 cgtcaccgta gatcccggtt acgccgaagc tggacgccag gcggcctgcc aattgatcgc
     8641 acaggtaacc gggcgcagcg aaccgcaaca aatcatcatc cccgccaccc tgtcctgatc
     8701 gtttcctgaa cgataaattg tgatcttcgc tgcatttcgg gaacgttctc gcttttaaat
     8761 ttttccgcgc aatatattct gcagccaacc aaaaatgtca tctgccatgg ggctttatga
     8821 tgagcaaaat aaaccaaacg gatatcgatc ggttgattga actggtcggc gggcgcggca
     8881 atattgcgac ggtgagccac tgtattactc gcctgcgctt tgtcctcaac caaccggcca
     8941 atgccagacc gaaagaaatt gagcaactcc ccatggtgaa aggctgtttc accaatgccg
     9001 ggcaatttca ggtggtgatt ggcaccaacg tgggtgatta ctatcaagca ctaatagcgt
     9061 caaccggaca ggcgcaggtt gataaagagc aggtaaaaaa agccgcccgg cagaatatga
     9121 aatggcatga gcagttgatc tctcatttcg cggagatctt cttcccgttg ctgcccgcgt
     9181 tgattagcgg cggtttgatc ctcggttttc gcaatgtgat cggcgatttg cccatgagca
     9241 acggtcagac gctggcgcaa atgtaccctt ccctgcaaac gatctacgat tttctgtggt
     9301 tgatcggtga agcgattttc ttctacctgc cggtcgggat ttgctggtca gcggtgaaaa
     9361 aaatgggcgg cacgccgatc cttggtatcg tgcttggcgt gacactggtt tccccccagc
     9421 tgatgaacgc ttatctgctc gggcagcagc tgccggaagt gtgggacttt ggcatgttca
     9481 gcatcgccaa agtgggctat caggcgcagg tgatcccggc actgttagcc gggctggcgc
     9541 tgggcgttat tgaaactcgc cttaaacgca tcgtaccgga ttacctctat ctggtggtgg
     9601 tgcccgtctg ttcgctgatc ctcgcggtgt tcctcgccca tgcgctgatt ggtccgtttg
     9661 gtcgcatgat tggcgatggc gttgcctttg cggtacgtca cctgatgacc ggcagctttg
     9721 ctccgattgg tgcggcattg tttggcttcc tgtacgcgcc gctggtgatc accggcgtac
     9781 accagaccac ccttgctatt gatttgcaga tgattcaaag catgggtggc acgccagtgt
     9841 ggccgctgat tgcgctgtcg aatatcgctc agggctccgc cgtgataggc attatcattt
     9901 ccagccgcaa gcacaatgaa cgcgagatct ccgtgcctgc cgctatctcc gcctggcttg
     9961 gggtcactga gcctgcaatg tacggcatca acctgaaata tcgcttcccg atgctgtgcg
    10021 cgatgattgg ttctggtctg gcaggattgc tatgcggcct gaacggcgtt atggcgaatg
    10081 gtatcggcgt agggggcctg ccgggaattc tctcgattca accgagctac tggcaggtat
    10141 ttgcgctggc aatggctatc gccatcatca tcccgattgt actcacctcg tttatctatc
    10201 agcggaaata ccgcttgggc acgctggaca ttgtttaatt ttcttcgggg cgcaattgcg
    10261 ccccctcgca tttgcaggaa taacgtaatg actaatcttc cccactggtg gcaaaacggc
    10321 gttatctacc agatttatcc aaagagtttt caggacacca cgggtagcgg caccggcgat
    10381 ttacgtggcg ttatccaacg cctggactat ctgcataaac tgggcgttga tgccatctgg
    10441 ctaaccccct tttatgtctc tccccaggtc gataacggtt acgatgtagc gaactatacg
    10501 gcgattgatc ccacctacgg cacgctggac gattttgacg aactggtgac gcaggcaaaa
    10561 tcgcgcggga ttcgtatcat tctcgatatg gtgtttaacc atacctctac ccaacatgcc
    10621 tggtttcgcg aggcgctgaa caaagaaagc ccttaccgcc agttttatat ctggcgcgat
    10681 ggcgaaccag aaacgccacc gaacaactgg cgttcaaaat ttggcggtag tgcgtggcgc
    10741 tggcatgcgg aaagcgaaca gtactatttg catctctttg caccagaaca ggcggatctc
    10801 aactgggaga atccagcggt acgcgcagag ctgaaaaaag tctgtgaatt ctgggccgat
    10861 cgtggggtcg acgggttgcg cctggatgtg gtgaatctga tctccaaaga cccgcgtttc
    10921 cctgaagacc tggatggcga cgggcgtcgc ttctacaccg acgggccacg agcacacgag
    10981 tttttgcacg agatgaaccg cgatgtgttt acgccacgcg ggttaatgac cgtaggtgaa
    11041 atgtcctcca ccagccttga gcattgccag cgatacgcgg cactgacagg cagtgaattg
    11101 tcgatgacct ttaattttca tcacctgaag gtcgattatc ccggtggtga aaaatggacg
    11161 ctggctaaac ctgactttgt ggcgttgaaa acattgttcc gccactggca acaaggaatg
    11221 cacaacgtgg catggaatgc cttgttctgg tgtaaccacg atcagccgcg cattgtttct
    11281 cgctttggtg atgaaggtga ataccgcgtg cctgcggcaa aaatgctggc gatggtgctg
    11341 catggcatgc agggaacgcc gtatatctac cagggcgaag agattggcat gaccaacccg
    11401 catttcacgc gcattactga ctatcgcgac gtggagagcc tcaatatgtt tgccgagctg
    11461 cgcaacgatg gtcgtgatgc cgacgagtta ttggcaatcc tcgccagtaa atcccgtgac
    11521 aacagccgca cgcccatgca atggagcaac ggcgataatg ccgggtttac ggctggcgaa
    11581 ccgtggattg gcctgggcga taactatcaa caaatcaacg tagaagccgc gctggccgat
    11641 gactcctcgg tgttttacac ctaccaaaag ttaatcgcac tgcgtaagca agaagccatc
    11701 ctgacatggg gcaattacca ggatctgctg ccaaacagcc ctgtattgtg gtgctatcgc
    11761 cgtgaatgga aggggcaaac cttgctggtc attgccaacc ttagccgtga gatccaaccc
    11821 tggcagccag ggcaaatgcg cggcaactgg cagcttgtga tgcataacta cgaagaagcc
    11881 tcaccacaac cctgtgccat gaatttacgg ccttttgagg ctgtctggtg gttacagaag
    11941 taaatcttcc ctaagccccg gtaatgccgg tcattccggg gttttgtctg tcagcatgtt
    12001 gtttttgttg atttttcaac cagcaaattc attaaaaaat ttacataacg ctgtagcgcc
    12061 cgtcatccgt acgctctgct ttttactttg agctacatca aaaaaagtta aaacatcctt
    12121 gatgcaaagc actatatata gactttaaaa tgcgtcccaa cccaatatgt tgtattaatc
    12181 gactataatt gctactacag ctccccacga aaaaggtgcg gcgttgtgga taagcggatg
    12241 gcgattgcgg aaagcaccgg aaaacgaaac gaaaaaaccg gaaaacgcct ttcccaattt
    12301 ctgtggataa cctgttctta aaaatatgga gcgatcatga caccgcatgt gatgaaacga
    12361 gacggctgca aagtgccgtt taaatcagag cgcatcaaag aagcgattct gcgtgcagct
    12421 aaagcagcgg aagtcgatga tgccgattat tgcgccactg ttgccgcggt tgtcagcgag
    12481 cagatgcagg gccgcaacca ggtggatatc aacgagatcc agaccgcagt tgaaaaccag
    12541 ctgatgtcgg gtccatacaa acaactggct cgtgcttaca tcgagtaccg tcacgatcgc
    12601 gacattgaac gtgaaaaacg cggtcgcctg aaccaggaga tccgtggtct ggtcgagcag
    12661 accaacgcct cgttactcaa cgaaaacgcc aacaaagaca gcaaggtgat tccaacccag
    12721 cgcgacctgc tggccgggat cgtggctaaa cactatgcac gccagcacct gctgccgcgt
    12781 gacgtggtgc aggcacatga gcgtggcgat attcactatc acgatctcga ttactcgccg
    12841 ttcttcccga tgttcaactg catgttgatc gacctgaaag gcatgctgac ccagggcttt
    12901 aaaatgggga acgccgagat tgaaccgccg aagtcgatct ctacggcaac cgcggtaact
    12961 gcgcagatta ttgctcaggt cgccagccat atttatggcg gcaccaccat taaccgtatc
    13021 gatgaagtgc tggcgccgtt tgttaccgcc agctacaaca aacatcgcaa gaccgcagaa
    13081 gagtggaaca tcccggacgc cgaaggctac gctaactctc gtaccatcaa agagtgctac
    13141 gatgccttcc agtcgctgga gtacgaagta aacaccctgc acaccgccaa cggtcagacg
    13201 ccgtttgtaa cctttggttt tggtctgggc accagcaggg aatcgcgcct gattcaggaa
    13261 tcgatcctgc gtaaccgtat cgcaggcctc ggtaaaaacc gtaaaactgc ggtgttcccg
    13321 aaactggtgt ttgcgattcg cgatggcctg aaccataaaa aaggcgatcc gaactacgac
    13381 atcaaacagc tggcgctgga gtgcgcaagc aagcgcatgt atccggatat cctgaactac
    13441 gatcaggtag tgaaagtcac cggttcgttt aaaaccccga tgggctgccg cagcttcctc
    13501 ggcgtgtggg aaaatgaaaa cggcgagcag atccacgatg gtcgtaacaa cctcggcgtg
    13561 atcagcctga acctgccgcg tattgctctg gaagcaaaag gcgatgaagc caccttctgg
    13621 aagctgctgg atgaacgtct ggtgctggca cgtaaggcac tgatgacccg tatcgctcgt
    13681 ctcgaaggcg tgaaagcgcg cgtcgccccg atcctctata tggaaggtgc ttgtggcgta
    13741 cgtctgaatg ctgacgatga tgtttctgaa atcttcaaaa acggtcgtgc gtctatttcg
    13801 ctgggttaca tcggcatcca cgaaaccatt aacgcgctgt tcggcggcga gcatgtttac
    13861 gacaacgagc agcttcgcgc gaaaggtatc gccattgttg aacgtctgcg tcaggcagtg
    13921 gatcagtgga aagaagaaac gggttatggt ttcagtctct acagcacgcc gagtgaaaac
    13981 ctgtgcgacc gcttctgccg tctcgatact gctgagtttg gcgtggtgcc gggcgtgacc
    14041 gataaaggtt actacaccaa cagtttccac cttgatgtgg agaagaaggt gaacccgtac
    14101 gacaagatcg actttgaagc gccttacccg ccgctggcga acggtggttt catttgctac
    14161 ggcgagtatc caaacattca gcacaacctg aaagcgctgg aagatgtctg ggattacagc
    14221 tatcagcatg taccgtatta cggcaccaat acaccgattg atgagtgcta cgagtgtggc
    14281 tttaccggtg agttcgagtg caccagcaaa ggcttcactt gcccgaaatg tggtaaccat
    14341 gacgcctccc gtgtgtcggt aactcgccgc gtgtgcggat atttaggtag cccggatgcg
    14401 cgtccgttta acgccggtaa gcaggaagaa gttaagcgcc gcgttaaaca tttggggaat
    14461 gggcagatag gttaatcttc actctgcgtt aagcaaatgc cggatgcggc gtaaacgcct
    14521 tatccggcct acatttggca acggctgtag gcctgataag acgcgacagc gtcgcatcag
    14581 gcattgatta ccggatgcag cctacacgcg gactgataag ccaggcgaaa taatgaatta
    14641 tcatcagtac tatcctgtcg acatcgtcaa cggccccggc actcgctgca ccctgtttgt
    14701 ctccgggtgt gttcatgaat gccccggttg ctataacaaa agcacatggc gggtaaattc
    14761 tggtcagcca tttaccaaag caatggaaga caagatcatt aaagatctga atgacactcg
    14821 tatcaaacgc caggggattt ccctctccgg cggcgatccg ctgcatccgc aaaacgtgcc
    14881 ggatattctg aaactggtaa aacgcatccg cgccgagtgt ccgggtaaag acatctgggt
    14941 gtggacaggc tataaactcg acgaactcaa cgctgcgcaa atgcaggttg ttgatttgat
    15001 taacgtgctg gtcgacggca aatttgtgca ggatttaaaa gacccttccc tgatctggcg
    15061 cggcagcagc aatcaggtgg tgcatcattt gcgatgataa gttccctgtt acctgagtgc
    15121 aggcaacagg gaaatgagga attaacgata cttctggtga taggcgttgc gggtcgtttt
    15181 cagttgctct gcagcagcct gcgcttcttt tactttacct tcatttgcca gcttcagcgc
    15241 gtcgtcaatc tgaccgacca gaatgtcgaa accgtggcgg aaatctttca tttccgggct
    15301 gtccggtgat ttatcttcga gcttcggcgg cgttgctttt tgcgcatcca gcgctgcggc
    15361 gcgcatcttc gttaacgcgt ctttgacttg cgtcgcgtta tcggcttttt cgaccacttt
    15421 taaattgtcg ttgagggttt ccatattgtc ttcaagatca gcggcaaacg acgccgaact
    15481 gaataccaac gaggagactg caagaatagc taacaggctt ttacgcattg ctcacttcct
    15541 tttttattta cacttcatcc ttcacactgc ctcatcgaca gctacgctca ttcgaccggg
    15601 tcacgtacta cgagtacgct ccccggctct cattcacttt ccgtctcgat gcagtgcgaa
    15661 tgattttgtg taatttttta gtttatagcg cggcaggtcg cgccagtttt tttactgtcc
    15721 ggcgattttc atctccggca acagcacaga accacactgt atattactac gtgtttcaat
    15781 atcgttaccg acggtgacaa tattgcgcca catatctttt aaattacctg cgatggtgat
    15841 ttcgctcacc ggatactgaa tttcgccatt ctctacccag aagcccgcag caccacggga
    15901 ataatcaccg gtaatggcac tcacgccctg gcccatcagc tccgtcacca ccagcccggt
    15961 gcccatctct ttgagcattt gttcgaagct tagcccttgt ccggcaatcc gccagttgtg
    16021 aataccgccc gcatgtccgg tgcttttcag cccaagtttt cgcgccgaat agctggtcag
    16081 cagccactga gtcaggatgc catctttaat aatatcgcga cgctcggtgc gcacaccttc
    16141 gctgtcgaat ggcgtcgacg ccagcccttt cagcagatgc ggatgctctt caatggtcag
    16201 ccagtccggc agaatttgtt tacccagcga atccagcagg aaggtagatt tacgataaac
    16261 cgatccaccc gctatcgccc ccaccagatg cccaaaaagc ccagttgcca cttcattggc
    16321 aaaaatgact ggcgctttca tggtggagag tttacgcggt aacaggcgcg ataaagtacg
    16381 gcgagcacag tcggccccaa cccactctgg cgtttgcaga tcgctcatcg cgcgcccaat
    16441 ggtgtaggcg taatcacgct ccatatcgcc attttcttcg gcaattacac agctggagag
    16501 cgaatgacgc gttgagcagt aaccctgcaa catgccgtgg ctgttgccaa aaactttgac
    16561 accgtagtgg ctgttaaagc tgccaccttc ggtattggtg atacgtttat ccgcctgtaa
    16621 tgctgcctgt tctgcgcggg ccgccagttc aatggcttca tccggggaaa cttccgcagg
    16681 gtggaacaag tcgagatctg gtgcgtcaaa ggccagcagc tctttgtctg ccaggccggc
    16741 acagggatct ggcgaggtat aacgggcaat atccagcgcc gcctgtacag tacgggcaat
    16801 ggcctgcggg cttaaatcgg tggatgatgc gctacctttg cggttctggt gataaacagt
    16861 gatccccagc gcgccatcgc tattgaattc aacattctcc acttcaccat aacgcgtgct
    16921 tacgctaatg ccggtggtct tgctgacggc aacttccgca ccgtccgatt tgcctgaggc
    16981 caactccagc gcagtggaaa ctgcttcttc cagaatcttg cgctgcgctt caacttgaga
    17041 gattactttc attgcaagtg ccataatgta gagagtttct ctgaagtcta acagagaacc
    17101 gtttttcagt gcgcatctta actggtaaca ttagcctctt tttttaagga gcctgagatg
    17161 actaagcagc ccgaagactg gctcgacgac gttcccggtg atgacatcga agacgaagac
    17221 gatgaaatta tctgggtcag taaaagtgaa attaaacgtg atgccgagga gctaaaacgc
    17281 cttggcgcgg aaattgttga tctggggaaa aacgcgctgg ataagatccc gttagatgcg
    17341 gatctgcgcg cggctattga gctcgcccag cgtattaaga tggaaggtcg ccgccgccag
    17401 ttgcagctca tcggtaagat gctgcgccag cgcgacgtag agcctattcg tcaggcgctg
    17461 gataagctga aaaaccgtca caaccagcag gtggtgctgt tccataaact ggaaaacctg
    17521 cgcgatcgtc tgatcgatca aggtgatgac gccatcgctg aagtattgaa tctgtggccc
    17581 gatgccgatc gtcagcaact gcgtacactg atccgcaacg cgaagaaaga gaaagaaggg
    17641 aataagccgc cgaaatccgc acgccagatt ttccagtatc tgcgcgaact ggcggagaat
    17701 gaagggtaat tttcgttgtt gatgtgagta tggtttgccg gatgcggcgt aaacgcctta
    17761 tccggcaatg ctatatcagg ctgaagccga attactgcgc agcttccgcc ttcttcgcca
    17821 gaccatccag cagtttctga tggatcccac caaaaccgcc gttgctcatc accagaatat
    17881 ggtcgccagg ctgagcggtt ttcaccacca tatctgccag cgtatccaca tcgccactcc
    17941 agtgtgcagg ctgaacgcag gcttctgcca cttctgccac ctgccacgga atatgcgccg
    18001 gttgcagcag gaagacttca tcggcacgac ctaatgaagg tgccagatcg tctttgcaga
    18061 tccccatttt catggtattc gagcgcggtt ccagcacagc aataatgcgc gccgtaccac
    18121 caactttgcc acgcagcgcc gccagcgttg ccagaatcgc cgtcgggtga tgggcaaaat
    18181 cgtcatatac cgtgacgcca ttcgcttcac cacgcaactc cagacggcga cgagcattaa
    18241 taaacgaacc cagcgcgtta gcggcatctg ccgacgctac accaacatgg cgagccgctg
    18301 caatcgccat caggccattg tgcatattat gttcgcctac cagcgaccat ttcacttcgc
    18361 ccactttttc gccatccagc aaaacttccc attcggaagc atcggtggtc agctttttcg
    18421 cctgccagtg accctgctca cccaccagct cctgctcgct ccagcagccc atcgccatgg
    18481 tctgtttcag gttgatgtca ttttccggcc agataatacg gccctgcccc ggaacgatac
    18541 gcaccagatg gtggaactgt ttctggatcg ctttcaggtc gtcaaagata tcggcgtgat
    18601 cgaactcaag gttgttgagg atcagcgtac gcgggcagta atggacaaat ttagagcgtt
    18661 tgtcgaagaa ggcgcagtcg tactcatccg cttcgataac aaagaagtcg ctttcgccca
    18721 gacgcgccga aacctcaaag ttccccggca caccgccgat cacaaatccc ggtttgtaac
    18781 cacactgttc cagaatccag gtcgccattc ccgcggtggt ggttttgccg tgtgtaccgg
    18841 caacggccag cacccagcgg tcgcgcagca caaaatcgtg cagccactgt ggacctgaca
    18901 tataagggat gtttttttcc agtaccgctt ccacacacgg atttccacgg gtcatggcgt
    18961 tgccaataat caccagatcc ggctgcggct cgagctggct ggcatcgtaa ccctgaatca
    19021 gctcaatgcc ttgcttctca agtaaggtgc tcatcggcgg atacacattg gcgtccgaac
    19081 ccgttacttc atggcctaac tggcgcgcca gcatcgccag accgcccata aatgtgccac
    19141 aaattcctaa aatatgaatg cgcatacgtc actatccttt tttaatctgc cgctcatttt
    19201 acgcatatgt cgggcaagtg agaaacgcat ttcaggataa tccgcaattt gctggcgcga
    19261 ttcacctgag cgcaacattg agattatttg ttaagattgt tgcggtcgct ttactccata
    19321 aacattgcag ggaaagtttt atgaaaacgt taggtgaatt tattgtcgaa aagcagcacg
    19381 agttttctca tgctaccggt gagctcactg ctttgctgtc ggcaataaaa ctgggcgcca
    19441 agattatcca tcgcgatatc aacaaagcag gactggttga tatcctgggt gccagcggtg
    19501 ctgagaacgt gcagggcgag gttcagcaaa aactcgactt gttcgctaat gaaaaactga
    19561 aagccgcact gaaagcacgc gatatcgttg cggggattgc ctctgaagaa gaagatgaga
    19621 ttgtcgtctt tgaaggctgt gaacacgcaa aatacgtggt gctgatggac cccctggatg
    19681 gctcgtccaa catcgatgtt aacgtctctg tcggtaccat tttctccatc taccgccgcg
    19741 ttacgcctgt tggcacgccg gtaacggaag aagatttcct ccagcctggt aacaaacagg
    19801 ttgcggcagg ttacgtggta tacggctcct ctaccatgct ggtttacacc accggatgcg
    19861 gtgttcacgc ctttacttac gatccttcgc tgggcgtttt ctgcctgtgc caggaacgga
    19921 tgcgcttccc ggagaaaggc aaaacctact ccatcaacga aggaaactac attaagtttc
    19981 cgaacggggt gaagaagtac attaaattct gccaggaaga agataaatcc accaatcgcc
    20041 cgtatacctc gcgttatatc ggttcactgg ttgcggattt ccaccgtaac ctgctgaaag
    20101 gcggtattta tctctaccca agcaccgcca gccacccgga cggcaaactg cgtttgcttt
    20161 atgagtgcaa cccgatggca ttcctggcgg aacaagcggg cggtaaagcg agcgatggca
    20221 aagagcgtat tctggatatc atcccggaaa ccctgcacca gcgccgttcg ttctttgtcg
    20281 gcaacgacca tatggttgaa gatgtcgaac gctttatccg tgagttcccg gacgcgtaat
    20341 ctcctgcgcc tctccacacg gagaggcgtt tttattgctg cgcaatgttg actcttgtca
    20401 ctggcgaact ctgacgattc tcccacagca ccgttaatcc acgctgtaat gctataaaaa
    20461 taaacaacaa aataccgatg gcgattttcg tccaccagga actcagcgtg ccatcaaagt
    20521 ttatgtaagt ctgaatcagt ccctgaatcg ccacgccaaa aagcgtccct aataccgttc
    20581 caacgccacc gctcaaaagc gtaccgccaa ttaccactga ggcgatagca tccagctcca
    20641 cacctacgcc cgccagcgca tatccggcct gggtataaat cgagaagaca atccccgcca
    20701 gcgttgccag tccggtggag agcatataaa tgcgaatagt ggtgctgcga gtggaaatcc
    20761 ccatcagatt cgccgacgtt gcgttgccgc caatggcgta tacctgatta ccaaaacggg
    20821 tacgatgcgc gaggaatatg ccgataacca ccaccgccaa catcagcagt cccatcgcac
    20881 ttaagcgacc gccgccaggg attttccacg caaggcttga gagcgtgtca taaatgggat
    20941 ggtttatcgg aatcgactct tccgaaacga gatagctgac gccgcgcaaa aagaacatcc
    21001 ccgcaagcgt aatgatgaac gccgggatct tcagggcgtc gatcagaagc cccataaatg
    21061 cgccgaaggc acagcccatc accagcacca gcggaaacgc cagcagcggc gagaggccga
    21121 aatcgccaat cacttttgcc agaaacacgc cggtaaaggc gatcaccgaa ccgacggaga
    21181 gatcgatccc accggagagg atcacaaagg tcatgccaac ggcaatgatc ccaagaaagg
    21241 cgttatcggt caggatattg cagatcactc ttgtggaagc aaaaccagga aactgcgtca
    21301 ggcagtagag atagcccagc acaaagacgc cgatggtgat cataagcggc aagttacgtt
    21361 ttatcacggc tacgcactcc tttaatcaga ctgataaagc gttgcgactg aacaatcagc
    21421 acacaaagca ccaccaccgc tttcaccacc tggttcatct ctggcggaaa gcccgaaagc
    21481 aaaattccgg tattcattcc ctgaataatc agcgccccaa ctcccgaaag cagcaggtta
    21541 aagcgaccgc ccatcagcga tccaccgcca atcaccaccg cgagaatggc atccagctcc
    21601 agccataatc cggcgttgtt ggcatcggca ccgcgaatat ccgccgccac gataatgccc
    21661 gcaatcgccg cacatagccc gctcaacacg taggtgagca tgacgatgat tcgtgtgttg
    21721 actccggcat tttttgccgc ccgaatgttg ataccaacgg cttcgataaa catccccagc
    21781 gccgttttgc gggtcaacag ccagaacagg agaagcgtca gcaccgcgat aatgaccggc
    21841 gttggcagga acaacagcga tccgctgcca aaccatgaga gatccggcga gttaaacgtg
    21901 acgatctgcc cggcggtgat cagttgcgcc acgccgcgcc cggcgaccat caggatcagg
    21961 gtggcgacaa acggctgaat tttgaggatc gctaccagta tgccgttcca caatcccgct
    22021 aggatgccgg tgcccagggc gcttaacaaa acaatcggca ggctgaatcc cgcgaccgtc
    22081 atcgcagccg ttgtggctcc ggcgatagcc atcaccgccc ccacagagag atcgatacca
    22141 ccggtggcga tcaccagcgt cataccaatc gccagtaacg caacgggagc tgcacggtta
    22201 agaatgtcga tagggctacc gaacaaacgc ccatcctgaa gcaccacctg ccagaaatgc
    22261 ggggctacca ggctatcgac cagcagcacc agcaacagtg ccgccagctg cggcatcccc
    22321 gtcggccagt gaaaacgcct ttttggcggc gtagtgtcag ggagagattg aggcatcaca
    22381 ctgttctcct tacgccgcaa tggcgttcat gatcgccgga acggaaagct gtgccagcgg
    22441 gatctccgcc acctgtttgc gatcgcgcat gatgatcacc cggtcggcat agcccaccag
    22501 ctcttccagt tcggaggaga tcaccagcag cgccagaccg tcagcacaca gcgtttcaat
    22561 caggcggatg atctcggcgt gggcaccaac atcaatgcca cgggttggct catcaaggat
    22621 cagaaattgc ggtcgggtca gtagccaacg tgaaagcagc actttttgct gattgccacc
    22681 ggagagaaat tcaatcggtt gttcagttga aggtgtgcga atgccaagct ggcggataaa
    22741 gcgttcggca atctcttgct gctctttgcg ggaaatgggt cgtagccagc cgcgctgggc
    22801 ctggagagcg aggatgatat tttcccgcac cgaggcggcg gcgatgatgc catcggtttt
    22861 cctgtcttcc gggcagaatc cgatgcccag tactgaagcc tgatgtggcg atcgcagggt
    22921 ttgcggtttg cctttgatca acgccgtgcc gctgtcagca ggtttgatac cgaagatcac
    22981 ttcggcggtt tcggtacgtc cggatcccag caagccagcc agaccgacga tctcgccggg
    23041 gcgcacttcg agatcaaacg gtgctatcgt tccttttttg ccgtaatttt tgaacgcggc
    23101 aacgggtttg tcgctcaaca atgttcgccc ggcacgctgt agcacgtggg tatccagctc
    23161 gcgccccagc atcattttta ccagttcgat ctgcggtagc tcgcgcgttt cccgacagcc
    23221 tacgaaactg ccgttgcgta agacagtgat ccgatcgctg acctgataga cctgatcgag
    23281 aaagtgagtg acaaagatca ggctgacgcc gcgatcgcgc aactgacgca tcaggccaaa
    23341 taatagctcc acttcctggg tgtcgaggct ggcggtgggt tcatcgagga tcagcacttt
    23401 ggcggagaga tcgatagccc ggcaaatggc gacgatttgc tgcatcgcga ctgaaaagcg
    23461 gttaagcggt tcgcgcacgt cgaggaagaa accgtaagat gccatcagtt cggtggcgcg
    23521 cttttccatc tctttacggc gtagaaggcc gaaacgtttg ggttcgcgac ctatgaatag
    23581 attatcagca accgacatat tgggtagcag gttgacttcc tgatagacgg tgccaatacc
    23641 gagttgttgt gcatgggcgg tatttttcgg tgagatagcc tggccttcca gccagatggt
    23701 gccgcgatcg gcgtggtata caccagttaa tgctttgatt agcgttgatt ttcccgcccc
    23761 gttttcacca agcagcgcca tgatttcgcc acggcgcagg ctgaaatcaa cgttgtctaa
    23821 cgctttgacg ccggggaaaa atttacttaa tccttcggtg cggaggatct cctggtgttg
    23881 gtcggtcgtc atggtttccc ccggtgtaaa gcgtgctccc agtaggcatg ataagacgcg
    23941 gtaagcgtcg catcaggctt cagtgcacca tcgccggatg cggcgcgagc gccttatccg
    24001 gcctacgaat cggcatcagg catagcaacc ctcaataccc catatttttc ttcttctcta
    24061 actcttcttt tgcggtatca ggcaggtaaa gggtggattt ggttaacgtc agcttttcag
    24121 gcatggtgcc gtcttttttg tatttctcca gcgcatcgaa agcgggtccc gccatgttcg
    24181 gcgtcagttc aacactggcg ttcgcttcgc catccatcat cgctttgtag atgtccggta
    24241 caccgtcaat ggaacccgtg aggatatctt tgcccggttt caggcccgct tctttaattg
    24301 cctgaattgc accaatcacc atgtcgtcgt tatgggcgta aaccatgcag atgtttttgc
    24361 cgttgttttc cgctttgata aagctctcca tgacttcttt gcctttactg cgggtgaagt
    24421 cacctgactg cgagcggata attttgatat ttggcgcatt cttaatggct tcggcaaagc
    24481 ctttcttacg gtcaatggcg acgctggccc caacggtgcc ctgcagctcc accacgttgc
    24541 atggtttgcc attcacttct tttaccagcc agtcaccgat caacttacct tcgaggatgt
    24601 tgtcggcagt aacggtggtc atatagagag atttgtcttt cacatcgatg gaacggtcga
    24661 gcaagaagac cgggatttcg gcatctttcg cctcttttaa taccggttcc caacccgtcg
    24721 caaccactgg agcgataaag atcgcatcca ccccttgtgc aacgaaggaa cgtaccgctt
    24781 taatctggtt ttcctgcttt tgctgaccat cggcaatttt caacgtaatt ccgcgttttt
    24841 cggcctcact tttcgccaca ttggtttctg cggcgcgcca gccggattcc gatccgacct
    24901 gcgaaaatcc tccggttaat ggagcggcca acgccataga cgacatggct gccgagactg
    24961 cagagactac aagtaagcgt ttccacatag ggttgtcctc gtcggggtaa ttattattgg
    25021 tgaaaagatg ttcgcgaaaa aactatagac aattcgttat gtaacggatt gcgttacatc
    25081 acacttcaaa aaagtaaata aaacgttaat cacaagtttg taatcgcttt catctcacta
    25141 tgaaaaatgc ggctacggtt atggattttc ctgctctgta taccgtctta aaactggcga
    25201 aaaaggaaaa tgaagacgaa aacaagcaaa gacattcggc gcgagttggc tataatactc
    25261 ggcacttgtt tgccacatat ttttaaagga aacagacatg agcttactca acgtccctgc
    25321 gggtaaagat ctgccggaag acatctacgt tgttattgag atcccggcta acgcagatcc
    25381 gatcaaatac gaaatcgaca aagagagcgg cgcactgttc gttgaccgct tcatgtccac
    25441 cgcgatgttc tatccgtgca actacggtta catcaaccac accctgtctc tggacggtga
    25501 cccggttgac gtactggtcc cgactccgta cccgctgcag cctggttctg tgatccgttg
    25561 ccgtccggtt ggcgttctga aaatgaccga cgaagccggt gaagatgcga aactggttgc
    25621 ggttccgcac agcaagctga gcaaagaata cgatcacatt aaagacgtaa acgatctgcc
    25681 tgaactgctg aaagcgcaaa tcgctcactt cttcgagcac tacaaagacc tcgaaaaagg
    25741 caagtgggtg aaagttgaag gttgggaaaa cgcagaagcc gctaaagctg aaatcgttgc
    25801 ttccttcgag cgcgcaaaga ataaataagt tcttctagcg caataaccct gaacgccggg
    25861 cttcggttag taagggtttt ttatgcccgc gataaataaa ctctctattc caccatcatt
    25921 attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca ccattttgct
    25981 gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt caaagcgtgc
    26041 ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg attgcagata
    26101 tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa aatcgcgaga
    26161 tacatcgcat aaaacaacgc cacccgaagg tggcgtgctt ttattattac ttatccctgt
    26221 ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga taaacgtaca
    26281 tccatgcact cccgtacggc gtctgaatca actggcgcgc gtattcaccg cccctggtgc
    26341 gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca ccgtgtaccg
    26401 ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg tagttatcga
    26461 tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct tgtttgtggc
    26521 gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga gcaaatccag
    26581 tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc gataacgtaa
    26641 cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca gacctggcaa
    26701 tacatagccg ctgaccacta cctgggagga gtcgcctact ccctgggtgt cgagcgctaa
    26761 attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt gcgcaacccc
    26821 cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct ccagcccttg
    26881 tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag agaagatctc
    26941 cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcaattacgt cgtcttctgt
    27001 agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg gaccagagaa
    27061 cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc gcccttcagg
    27121 gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt taagatcgcc
    27181 cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa cgtggacaat
    27241 caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca ggttatcgtt
    27301 aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt gcaccacgat
    27361 gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg tagcctcgaa
    27421 tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc gcactttact
    27481 gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg catcaccgtt
    27541 caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc gcataccgtt
    27601 aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc cgtcgatatc
    27661 cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac cacccagacg
    27721 caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg ggtttatcat
    27781 cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc cttgcggatc
    27841 ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg tccagcccaa
    27901 ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg ccaccggcag
    27961 cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg tgatactgcc
    28021 ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg tgaagatacc
    28081 gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga agcggttgag
    28141 attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc acagttccgc
    28201 attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat tgcggtaatc
    28261 cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaaac gggtattgct
    28321 aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat taagctgccc
    28381 ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc tgcctttggc
    28441 attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa cgcgtacgtc
    28501 gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa cctgcgccac
    28561 ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt gtggcgcttc
    28621 caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg gcagcgcgtt
    28681 atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc ccagctcgcc
    28741 tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca tccactgcat
    28801 ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga cgttaccggt
    28861 cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac cgcgggtttt
    28921 aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt taatgccatt
    28981 aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg ccttgagttc
    29041 agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct gttcattacc
    29101 tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg ccgtacgcat
    29161 agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat ccgcctgata
    29221 ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca cgttgagtgg
    29281 caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg gaccggaaag
    29341 gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct tcaccttttc
    29401 acctttcaac ggctccacgt tcagtgtgct gttgaggatg atatccaccg gccagttgtc
    29461 tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga tatccagggc
    29521 gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc gcacggtgat
    29581 gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt tcaggttaag
    29641 cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat ctttcagcgt
    29701 ttcgccgagc ggcttttcat ccggctgcgg attttcaatc ttcggttcga cgacctcttc
    29761 ctgcgccact tccgccactt tcggcagagc aatcagcagg cctttcagcg acgtcggttt
    29821 cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca ccgacaccgt
    29881 ggtgtcatca atcttgatgt tgacgttatc cagcgccacc cgtgtcaggg tgatgggata
    29941 cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt cagaaggagg
    30001 cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat taatacaaac
    30061 gctactgttc cacaggcact caagaccgac cgccagatgc agattacccg cttttaccgc
    30121 cacgcctggc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc cgccggtgac
    30181 gttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa gatgcaggcc
    30241 gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa cgataaccac
    30301 gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat gtaaaactgt
    30361 aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat tggcccgacc
    30421 ggcgattccc agcgtacgcc gcccccggta ccggttttaa agtcgctgcg gcgaatatcg
    30481 cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt cacgttgtac
    30541 tggtattcca gcgatccggt tatcagcttc gaggcccctt tcaggtcacc gttggcgtat
    30601 ttcggggcga tagatttata tttgtagccg cgaatactac gatcgccccc ggcgaagaaa
    30661 cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag cgtgccgcgt
    30721 gtcacaaaac gatggcgatc gtacagtgtg cggatccaga cgttctgcgc ctggaaaacg
    30781 gagaaatcga catctgaacc ccaggccgtg ttggagtagt cgatagagta gcgttgcgag
    30841 tcgccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat caccccagga
    30901 taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag gctccagcgc
    30961 aggttaatgg cacgctgcca gtcgctggag agatcccagt agcgagaagc caccagcgta
    31021 gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg caccaaataa
    31081 tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag gatctgttcc
    31141 ggcgcggaaa tactggtact ggtggtcaga ctgtgaccat aagagttcat ccacggcttt
    31201 ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc ggtttcgatg
    31261 gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt ttcgcgcgct
    31321 ttatcaaatt gcggtgccac taccaccgaa ttaaaccagc cggtagcaga aagtcggcgg
    31381 ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaagcgg caccagattt
    31441 tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa gcggtaacgt
    31501 tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc aatgcccagc
    31561 tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact ggttaaggac
    31621 tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg gcgagtatcg
    31681 agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac atcggtgccg
    31741 ccaattaaca ccggtacacc tggcgtgact ttggcgatca atacctgccg ccctttcttt
    31801 ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc tttcagacct
    31861 tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac ttcatcactt
    31921 tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa cccctcgacc
    31981 tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct tacacagcat
    32041 aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctacc cctggaacgc
    32101 cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta aaccccaaat
    32161 atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa tacgcctctt
    32221 gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag tttatttgat
    32281 aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc gatgcccgta
    32341 gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg aatggagatt
    32401 gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca gttacccggc
    32461 gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac ttatcgggaa
    32521 gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga tccttccgtc
    32581 atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc ccagggaatg
    32641 cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct gaccccagaa
    32701 caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct tgccgccgat
    32761 gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta tgccgaagat
    32821 gaccaccagc aatatctgca taaaaaccca tatggttact gtggaattgg cggaattggc
    32881 gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt gataaagtgc
    32941 tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat cgtccaaatt
    33001 caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca gtattgcatt
    33061 tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc tatactagcc
    33121 actgaaaatg tcggttcact ttcttcgaat cggctttcaa tgtgtatttc acacaaatta
    33181 atcaactttc ccttccgagg atctggcctg aaaggtcgga taagatatgt taaacagtat
    33241 tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg agatctcgct
    33301 tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata taaatgccca
    33361 acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc aaatcggtct
    33421 gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc cagcttttca
    33481 cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct ttattctctc
    33541 tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga aacgcatcgg
    33601 tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct tctgcctgta
    33661 cgtttgcacc ccactggtgt ggttcttcaa cggcctggcg aacatgatct tccgtatttt
    33721 caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg cggtagtgga
    33781 agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg aaaacgtctt
    33841 tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg tgatttggtt
    33901 tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc cgcactctaa
    33961 gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt ctaaagacct
    34021 gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac aaattcgcaa
    34081 cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt ttaaaaccgc
    34141 aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga tcatcaccct
    34201 caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg aagagcagat
    34261 tgtcgcccgt gatgagaact catggctgat tgacggcggc actccgattg acgacgtcat
    34321 gcgcgtgctg gatattgacg aattcccgca gtcgggcaac tacgaaacca tcggcggctt
    34381 tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat tcgccggcta
    34441 caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg tgacccggat
    34501 cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata aagaagaaag
    34561 cgtcgcgtaa ccccccagaa acatcaacgg ctcctgaatc aggagccgtc ttattactgc
    34621 atagcacttt ttactgcata gcactttggt taagccatct ctgtttgcag acgcataacc
    34681 tgacggttaa cttcggacat cactgacaaa tgcagcttat ccttcacttt tgggataaga
    34741 atcttaccct tatcaaattc aaaagcgcca acgtccttga tgtataaccg tccacggaac
    34801 aggatcttta cgtacttcgc cacctgaagt gggttgtacc gttggaaaat tttcattgtt
    34861 atctcctgct gagtattacg cctgtgcggt gccacaatcg gcccaactat tatgaggcgc
    34921 aaattttaat gcttagtgac tatagactat ccgggcaatg tttccaccgc gtataacttt
    34981 ttttacctaa aggttacaat tattcagaat tatcttttta ccgaagcgcg gtcttcagta
    35041 taagcattca tttttcatat gatttgtgcg cttgaccgca aactggcatc acacttgcgg
    35101 gaaattcgat aaatagcaca tatgattaaa actcagaccc aagtggtcgg atcacctgca
    35161 tatcataaga aggaaacacc atgaccctac gcaagattct ggcactcacc tgcctgctgt
    35221 tgccgatgat ggcttccgca catcagttcg aaaccggtca gcgagtgccg ccgattggca
    35281 ttaccgatcg gggcgagttg gtgcttgata aagatcagtt tagctacaaa acctggaaca
    35341 gcgcgcagtt agtgggaaaa gtgcgagtac tgcaacatat tgctggtcgc acctctgcaa
    35401 aagagaaaaa cgcgacgctg attgaagcga ttaaatcagc gaagttaccg cacgatcgtt
    35461 accagaccac cactattgtt aacaccgacg acgcgattcc gggttcaggg atgtttgtgc
    35521 gcagcagtct ggagagtaat aaaaagcttt atccctggtc gcagtttatt gtcgatagca
    35581 atggcgtcgc gcgcggtgcc tggcagctgg atgaagaaag ttccgctgta gtggtgctgg
    35641 ataaggatgg tcgcgtgcaa tgggccaaag acggggcgct cactcaggaa gaggtgcagc
    35701 aagtgatgga cctgctgcaa aagttgctca aataatggca acaacataga ttctgcaaaa
    35761 ataattttcc cctatcgtta tctatgggat aggggaatta agtctctgag ttcttacgcc
    35821 gaactcatac tggtaacgct agttgctctt ttttaaacgc ctcaatggtc gacttatatt
    35881 tctcgcaaat cttttctttg gtaatattac tatctgcggc cagattcgca cttactatcg
    35941 catccacttg ttcggctaat tccacttgtt ggcaacctgc attaaccaga aaattataaa
    36001 taagggagta atcttcatcc tccaaaacaa cagaggaacc attacgaaaa cgttcatccc
    36061 ctccatagat tttaagcgca aagcccaaag attctatgac tatggcaaaa ttatcatcaa
    36121 ttattttttc gtgaatatac catccatcgc attctttaat tccacctcct ggtatttgga
    36181 gttcaaattt accccgtttt aatttgctta ctatatcctc caataaggca taacctaaag
    36241 acacgtcacc aatcgcctgg tgaatataac ctttaatcct tttcggtatg tcagactgtt
    36301 ttttctgcag aatatcacag gcatacaatc ttctgttttg ttctaacgga aactcgcggt
    36361 ttttgaaatc catgaatgag ttatactcat ttattgttat gggaatttct tcgtattcaa
    36421 atccactacc tcgcaatgtc attgtatatt cggcaccgtt atctttaagg ctgaaactga
    36481 taaggaactt atccatggcg ttaacagttc cattttttga tgaaagtacc tgataactat
    36541 ttttatcaat ctgagttgcg caacgaaaga actccctcaa agttaacagg ctatcataat
    36601 tcgaaatcca atcttttttc ttaaatgcat gctggatatt gttaaaaaaa ttgtttgaat
    36661 gtgtttttgt atacgatgac tttacaaata actccaccgc accaataaaa ttattgtgtc
    36721 tgatcctggg taacatatga caaccttatt ttatttggtt attttatgtc gccattaaat
    36781 atctcattag agaagtaaac tatcaaaaaa cgctcattat taaaatgtaa cttattagca
    36841 attgaatttt tgcactttaa atacaatctc tttttattta taacttttta ggttgggaga
    36901 aatcaggatg gcagaatcag gaaatacact gtttctgcca tctgaattta gtaaatagac
    36961 actctgaacc ccggattcag gaacgactcg cgcggagtgt aatccagcgg tttaccctgc
    37021 cagtcgtgaa cgtgcgctcc ggcagctgca gccacagcat gtccagcggc ggtgtcccaa
    37081 atattcgttg gtccgaagcg cgggtacagc tgcgcctgtc cttccgccac caggcagaat
    37141 ttcagcgaag agccgatgga cgtggtctga tgttcgccaa gctgttgcag atactctttc
    37201 agctccgcat ctgcatggga acggctgata accaccagcg gcgggcgcgc atcgcggacc
    37261 tgaatctgct tgcgcacacc gcactcttct ttccaggcct tgccttctgc cgcgctgtac
    37321 attacgttca ttaccggcgc atacaccaca cctaaaatcg gtttgccatg gtcaatgagc
    37381 gcaatgttaa cggtgaattc gccattacgt ttaataaact ctttagtacc atccagcggg
    37441 tctaccagcc agtaacgctg ccagtgctga cggacttccc aaccgggagg atcttcttca
    37501 gaaaggaccg gaatatccgg tgtcagcgta cgtaaaccgt ccatgataac ggtgtgtgcg
    37561 gcaatatccg ctgccgttac cggagaattg tccgctttgc tgacgacgtc catcggtttc
    37621 gtcccgtcgt agacctgcat aatggcatcg cctgcattcc gtgcaagctg gcatacttga
    37681 tctaacattt ctccacctcg tctctgtgag cggtgttaac ttattgtttt acttataccc
    37741 tatcgttaat gaatgcgcca actgtgatag tgtcatcatt ttcaaagcgt aaaattgtgg
    37801 cattcttcac tgttctataa gtaagacgtt tattctccct tttctttcgt attcccgatg
    37861 ataaaaggat gtccctgatg attaagttta gcgcaacgct cctggccacg ctgattgccg
    37921 ccagtgtgaa tgcagcgacg gtcgatctgc gtatcatgga aaccactgat ctgcatagca
    37981 acatgatgga tttcgattat tacaaagaca ccgccacgga aaaattcgga ctggtacgta
    38041 cggcaagcct gattaacgat gcccgcaatg aagtgaaaaa cagcgtactg gtcgataacg
    38101 gcgatttgat tcaggggagt ccgctggcag attacatgtc ggcgaaagga ttaaaaacag
    38161 gtgatgttca tccggtttat aaggcgctga atacgctgga ttatacggtc ggtacactcg
    38221 gcaatcatga atttaactac ggtctggatt acctgaaaaa tgcgctggcg ggagcgaaat
    38281 tcccttatgt aaatgccaac gtcattgacg ccagaaccaa acagccaatg tttacaccgt
    38341 atttaattaa agataccgaa gtggtcgata aagacggaaa aaaacagacg ctgaagattg
    38401 gctatattgg cgtcgtgccg ccgcaaatca tgggctggga taaagctaat ttatccggga
    38461 aagtgacggt gaatgatatt accgaaaccg tgcgcaaata cgtgcctgaa atgcgcgaga
    38521 aaggtgccga tgtcgttgtc gttctggcac attccgggct gtctgccgat ccgtataaag
    38581 tgatggcgga aaactcagtt tattacctca gtgaaattcc gggcgttaac gccattatgt
    38641 ttggtcatgc tcacgccgtt ttcccgggta aagattttgc tgatatcgaa ggggctgata
    38701 tcaccaaagg cacgctgaat ggtgttccgg cggtaatgcc gggcatgtgg ggcgatcatc
    38761 ttggggtggt cgacttacaa ctcagtaatg acagcggtaa atggcaggtg acgcaggcga
    38821 aagcggaagc tcggccgatt tacgacatcg ccaataaaaa atccctcgcg gcggaagaca
    38881 gcaagctggt agaaacactc aaagccgatc acgatgccac acgccagttc gtcagcaagc
    38941 caatcggtaa atctgccgac aatatgtata gctatctggc gctggtgcag gacgatccga
    39001 ccgtgcaagt cgtgaacaac gcgcaaaaag cgtatgtcga acattacatt cagggcgatc
    39061 cggatctggc aaaactgccg gtgctttcag ctgccgcacc gtttaaagtt ggtggtcgca
    39121 aaaatgaccc ggcaagctat gtggaggtgg aaaaaggcca gttgaccttc cgtaatgccg
    39181 ccgatcttta tctctacccc aatacgctga ttgtagtgaa agccagcggt aaagaggtga
    39241 aagagtggct ggagtgctcc gccggacagt ttaaccagat tgatcctaac agcacgaaac
    39301 cacagtcact catcaactgg gatggtttcc gcacttataa ctttgatgtt attgatggtg
    39361 tgaattatca gattgatgtt acccagcccg cccgttatga cggcgagtgc cagatgatta
    39421 ataccaatgc ggaaaggatt aagaacctga cctttaatgg caagccgatt gatccgaacg
    39481 ccatgttcct cgttgccacc aataactatc gcgcttacgg cggcaaattt gccggtacgg
    39541 gcgacagcca tatcgctttt gcttcaccgg atgagaaccg ctcggtgctg gcagcgtgga
    39601 ttgctgatga gtcgaaacgt gcgggggaaa ttcacccggc ggcagataac aactggcgtt
    39661 tagcaccgat agctgccgat aagaaactgg atatccgttt cgaaacttcc ccgtcagata
    39721 aagccgcagc gtttattaaa gagaaagggc agtatccgat gaataaagtc gcgaccgatg
    39781 atatcgggtt tgcgatttat caggtggatt tgagtaagta aaacacttct ttttcggcct
    39841 ataaatcatc aatcgcatcc ggcatttatt ggcggatgcg atgctggcgc atcttatccg
    39901 ccctacaagt catacaccgt aggccgcgca tccggcagtt atgccgcacg ttcatcccgc
    39961 accgccaaca cctcgggcaa attcaactca atccagtccg ccagtgcagc aaccttttcg
    40021 ctcacctgct cgcccagcgg cgtgaggctg tattcaacat gcggtggcac caccggatac
    40081 gcgatacggt taagaaaacc atcctgttct aacgcctgta acgactgcgc aagcatcttt
    40141 tcgctcaccc cgccgatttt gcgccgcagg tcgctaaagc gatgagtacc ttcgcgtagc
    40201 gccaccagaa tcaacacccc ccaacggctg gtgacgtgtt tcaacacctc gcgcgacggg
    40261 cactgttccg caaagagatt accctctttc agttgttgcg acaggctaac ctgactcatt
    40321 tcatacttac ctttttgtac gtacttacta aaagtaagtt taggtgttag cgtatttaaa
    40381 cacaagacaa aacgatggag acttcccatg atcgctatta ctggtgccac tggccaactt
    40441 ggtcactata ttattgaatc cttgatgaaa acggttcctg ccagccaaat agtggctatc
    40501 gttcgtaatc cggcaaaagc ccaggctcta gcagcacaag gcattaccgt gcgtcaggct
    40561 gactacggcg atgaagccgc actgacatct gcacttcagg gagtggaaaa actactgctg
    40621 atctcttcca gcgaagtggg tcaacgtgcc ccgcagcatc gtaatgttat taatgccgca
    40681 aaggcggctg gcgtgaaatt tatcgcttat accagcctgc tacatgcaga tacctccccg
    40741 ctcggcctcg ccgatgagca cattgagacg gagaaaatgc tggctgattc tggcatcgtt
    40801 tacaccctgc tgcgcaacgg ctggtacagc gaaaactacc tcgccagcgc cccggcagca
    40861 ctggaacacg gcgtatttat cggtgcggcg ggcgatggca aaatcgcctc agcaacgcgg
    40921 gcagattatg cggcagctgc ggcacgcgtg attagcgaag ccggtcacga aggcaaggtt
    40981 tacgaactgg cgggcgatag tgcctggacg ttgacacagt tagcggcaga gctgaccaaa
    41041 cagagcggca aacaggttac ctatcaaaat ctgagcgaag ccgatttcgc cgcggcactg
    41101 aaaagcgtcg gactgcccga cggactggcg gatatgctgg cggattctga cgttggcgca
    41161 tcgaaaggtg gtctgtttga taacagcaaa acgcttagca aattgattgg ccgcccaacg
    41221 acaacgttag ccgaaagcgt aagccatctt tttaatgtta ataactagtt aattaaagtg
    41281 gcatcctccc gcatcctctc tgataatgac gggatgccgg gagcaatcat gtctgcttcc
    41341 tgaactttct tctgacagac caatggatgc cagtaatgat tagcggcgtg ctgtacgccc
    41401 tgttagcagg gttgatgtgg gggcttattt ttgtcgggcc gttgatcgta ccggaatacc
    41461 cggcgatgtt gcagtcgatg gggcgttatc tggcgttagg gttaattgcg ctgcccattg
    41521 cctggctggg acgcgtgcgt ctgcgtcagt tggcgcgtcg cgactggctt accgccttga
    41581 tgctcaccat gatgggcaac cttatctatt acttctgcct tgccagtgcc attcaacgta
    41641 ctggcgcgcc tgtttccacg atgattatcg gcaccctgcc ggtggtgatt cccgtttttg
    41701 ccaatctgct ttatagccag cgcgacggca aactcgcatg gggaaaactc gccccggcac
    41761 tggtttgtat tggcatcggc ctggcgtgtg tgaatattgc tgagttaaac cacggactcc
    41821 ccgattttga ctgggcacgt tatacctctg gcatcgtgct ggcgttagtt tccgtggtct
    41881 gctgggcgtg gtatgccctg cgcaacgccc gctggctgcg ggaaaatccc gacaaacatc
    41941 cgatgatgtg ggcaacggcg caggcgctgg tcacactgcc ggtttctctc atcggctatc
    42001 tcgtcgcctg ttactggctg aatatacaaa cgccggactt ctccttacct tttggccccc
    42061 gtccgctggt gtttattagt ctgatggttg cgatagccgt gctttgctca tgggttggcg
    42121 cactctgctg gaacgtcgcc agccagcgat taccgacagt gattctcggg ccgctgattg
    42181 ttttcgaaac gctggcaggt ttgctgtaca cctttttact ccgccagcaa atgccgccgc
    42241 taatgacgct gagcggtatc gcgctgttag tgattggcgt ggtcattgcg gtcagagcaa
    42301 aaccagaaaa acctttaact gaatctgtct cagaaagttg acacgctggc agtgagttaa
    42361 ataagcctct gctacgtaag ggttatagct tttgccttaa agatgcattt aaaatacatc
    42421 ttatattatt aagaatgagg tatcagctat ggcttatcgc gaccaacctt taggtgaact
    42481 ggcgctctct attcctcgcg cttcagctct gtttcgtaaa tatgatatgg attactgctg
    42541 tggcggtaag caaacgctgg cgcgcgcggc ggcacgtaaa gaactggatg ttgaggtcat
    42601 tgaagctgaa ctggcaaagc ttgctgaaca accgattgag aaagactggc gtagcgcccc
    42661 gctggcagaa atcattgacc atatcatcgt gcgctaccac gatcgtcacc gcgagcaact
    42721 gccggagctg atcctgcaag cgactaaagt cgagcgcgtt cacgccgaca aaccgagcgt
    42781 gccaaaaggg ctgacaaaat acctgactat gctgcatgaa gagctatcca gccacatgat
    42841 gaaagaagag cagatcctct tcccgatgat caaacaaggc atgggcagcc aggcaatggg
    42901 accaatcagc gtgatggaaa gcgagcacga tgaagcgggc gaactgctgg aagtgattaa
    42961 acacaccacc aataacgtca caccgccgcc ggaagcctgc accacttgga aagcgatgta
    43021 taacggtatt aatgaactga ttgatgacct gatggatcac atcagcctgg aaaacaatgt
    43081 actgttccca cgcgcgctgg cgggtgagta atctaaaagc tggatggcat tgcgccatcc
    43141 agcatgataa tgcggttatt tccgcagttc agcagcccgc ttcttaccaa taaacaacca
    43201 gcccagcccc agtgcgataa accacagcgg ggtgaccaac agcgcctggc gagtgtcatc
    43261 ttccagtgtc agcaacacga ccacgaacac aaagaacgcc atacataccc agcacatcag
    43321 cttgccgagc ggcatcttgt agatcgactt ctcatgcaga tgaggacgct gtttgcggta
    43381 aaccaggtac gagcaaagga taattgtcca gacgaacata aacagaatcg cggaaacggt
    43441 tgtaatcatc gtgaacgcgc caatcacgct aggattcaca tacaacatca ccacgccgcc
    43501 gagcagacag atacacgaga aggtcaaccc tttcgcgggt actgcgcgct tagaaagttt
    43561 agcgaacgct ttcggtgcca caccttcctg cgccagacca aacagcatac ggctggtaga
    43621 gaagacgccg ctgttagcgg aagacgccgc agaggtcagc accacaaagt tgatcacgct
    43681 ggcagccgca ggcaaaccta ccaacacaaa cagttcaaca aacgggctct tctccgggac
    43741 taccgaactc cacggcgtca cggacataat cacaatcagc gcgaagacgt agaacataat
    43801 gatacggatc ggaatggagt taatcgcgcg tggcagtgat ttctccggat ctttggtttc
    43861 cgcagcggtt gtacctacca gctcaatccc tacgaaagcg aaaaccgcta tctggaatcc
    43921 ggcaaagaag ccacttaaac ctttcgggaa ccagccgcca tcattccaca aatgcgcgaa
    43981 tgacgcttcc acaccagtcg gtgactgaaa gtgcatcgcc accataacca ggccgacgac
    44041 aatcagcgac acaatggcga cgattttgat catcgcaaac cagaactcca tctcaccgaa
    44101 cattttcacg gtggcgagat tgagcgtcag cagcagcact atcaccgcca gcgaggcgac
    44161 ccagtcggag agatcgggga accagaactg agcataagca gtgatcgcca ccacgtctgc
    44221 cataccggtt acaacccagc agaaccagta agtccagccg gtgaaatatc ctgcccacgg
    44281 cccgagtaaa tcggaagcga agtcactaaa agatttgtat tccagattcg aaagcagcaa
    44341 ttcccccatt gcccgcatca cgaaaaagag cataaaaccg atgatcatat aaacgaaaat
    44401 gatcgacggc ccggcaaggc taatcgtttt gccggacccc ataaacaacc ccgtaccaat
    44461 ggcaccgcca atggcaataa gctgaatatg tcggtttgtg agattgcgcc gtagcgactg
    44521 ttcagccgga gcctgatcat cggcaacgac ttttacctga tctaccatgt ttttttcttc
    44581 ctgtacctgt ctgtgttgtt caggctctac ggcctttagt cagtctatga tacgacgaat
    44641 ctgtatcggg actcatcgat attaggtaac aatacgcggg atgaatacta agatttagat
    44701 ctaatgttaa ttttatgttt aaagtgagtg tcatatcacg gtgataacgc gaaacagctc
    44761 acaaaaatac acgcttacgg ggtctttgca acacaaaata gcgccgtttg gcaaactgaa
    44821 gggtttattg ctgaatgcct gctcccctct ggttcgaggg gaacagaatg cgctgcttag
    44881 aggatttcca gcagttcgac ttcaaacacc agggtgctga acggagggat ggatgcgcct
    44941 gcgccgcgct cgccatatgc cagttcctgc gggatagtca gttcccattt agaacctacc
    45001 ggcatcagag tcagtgcttc aatccagcca gggatcacgc cattaaccgg gaattcagcg
    45061 ggttcaccac gagcaacgga gctgtcaaac acggtgccgt cgatcagttt accggtgtaa
    45121 tgaacacgaa cgcggtcggt acgtgccgga attgcgcctt caccctggtt gatcacgcgg
    45181 aattgcaggc cagattcggt gctattcaca ccttcttttt tggcgttttc ttccaggtat
    45241 ttcacacctt cagcagccat cgcctggaaa cgctgacgac gaacggcatc ggcgcgttcg
    45301 tggatttcac gcagcgcgcg atgcaccaca tcaaccggca cagccggatg tttgccttcc
    45361 agcgcatcgg caatacctgc aaccagtgct tctggcagca gcccttccag gccagattca
    45421 ctcagttgtt gccctacctg caaaccaatg ccgtagcttg cttgcgcttc gatggtgtca
    45481 aaagttgggg tggtcatcgt atttcctttc ttagggtata aaagtagcgg gcagcatatc
    45541 agccacactt ctttgggtaa aactttgtcg caggaatgat gacaaatccc ggggaaaaag
    45601 aaacaatagc aatatcccgt atgaatataa taaaggcggg tttaccacgg acatcatagc
    45661 gttagcttat tcataggcta tgattgagga acaagacgcg gagcaggagg aaaccatgcc
    45721 cgggcgcttt gaactaaaac caaccctgga gaaagtctgg cacgcgccgg acaattttcg
    45781 cttcatggac ccgctgccgc cgatgcatcg tcgggggatc attattgccg ccattgtatt
    45841 ggtggtcgga tttctgcttc catctgatga tacgcccaac gcgccggtgg tgacgcgtga
    45901 agcgcagtta gatattcagt cgcaatcaca accgccgaca gaagaacagc tgcgggcgca
    45961 attagtcaca ccacaaaatg atcctgatca ggtggctccg gtcgctccag aacctataca
    46021 ggaaggccag ccagaagaac agccgcaaac tacccaaacg caaccattcc agccagatag
    46081 cggaattgat aatcaatggc gctcttaccg tgtcgaaccc ggtaaaacga tggcgcagct
    46141 gttccgcgac cacggactgc ccgctaccga tgtctatgca atggcgcagg tagaaggcgc
    46201 gggtaagccg ctgagtaatt tgcaaaatgg gcagatggtg aaaatccgcc agaacgccag
    46261 cggcgtggtg acgggcttaa ccattgatac ggggaataat caacaggtcc tgtttacccg
    46321 ccagccggat ggcagtttta ttcgtgcgcg gtagatgaag ggtcaaccaa aagccagcta
    46381 aggattgtct ctttgatgac cttacgtctg gcttccagtg cctggggtga aaccatactg
    46441 atgtgataaa ccaggccaag cgtatgctgg ttgatcaaat aatatccccc aagcgcagca
    46501 atattaatat tcacctgcaa tgggtcgata tctggcttaa aaatattgtg tttcttccct
    46561 tcatccaata aggattccat taactgcaag tgcgcatggt taatttctaa gagacgcagt
    46621 gattttgcat aatgcacacc tttactttga ttttcactat gcacaatttt taaaaaccaa
    46681 gggttagcaa tataataatc ccatgtaaaa tcaataattt tctcaatggc tttttgtggc
    46741 ggaagatctg agaaatcaag ggccttttct ttttgtcgaa tatcgttcca ggtatattcc
    46801 agcacttcga taaataaatt ttctttatta ccaaagtggt gataaaccgt ctgtttacta
    46861 caccctgctg cctttacaat attatctact ctggcccctt cataaccata ttcggcaaat
    46921 tcattaaccg cacatagcag caatttttcc ttcaggctct gtacgttgct ggtatcaata
    46981 tacataacgc ttccccacga cctcttcgtg gtgactatca taacctgatt tgatttcagc
    47041 ttcagaaaaa agggcaaaaa cttgtacttt catcaggtta tcaaatgtcc tgtgatcgcc
    47101 atcgcatatc aaccaactag atggttgatc tttttatcct ccagtcgcaa gctaatcatc
    47161 accaaggaaa caaaattcaa aaaagagagc tattgtatga aatccactcg tcctgttgcc
    47221 gtgattaccg gagccgctcg cggtattgga aagggctgtg cgcaggagct tgctcgtggc
    47281 gggtttaacc tgctgattaa cgatcgtccc gatgccgaca gcgtagaaaa actgcatatc
    47341 acacaacaag aatgtttggc ggaaggcgtg gaagtgattt gcttccctgc tgatgtcggt
    47401 gatctatctc tgcatgagga gatgctcaac gcggcgcaaa accagtgggg gcggctggac
    47461 tgtctgctca ataacgcggg gatttcagtg aaaaaacgcg gtgacctcct cgacctcgaa
    47521 ccagacagct tcgatcaaaa cattgccatt aacacccgcg cgccgttctt ccttgctcag
    47581 gcattcagta agcgcttact ggcacagccg aaaccggaag cggagctgcc gcaccgctcg
    47641 attattttcg tcagttccat caacgccatc atgctggcga tgaatcgcgg cgaatacacc
    47701 attgccaaaa ccgccgtttc tgccgctgct cgtctgtttg ctgctcgcct ttgtaatgaa
    47761 caaattggcg tttatgaagt gcgccctggt ctcatcaaaa ctgatatgac tattccggca
    47821 acggcttatt acgacgaact gattgccaaa ggtctggtgc cgtggggacg ttggggttat
    47881 ccggctgata ttgcttccac cgtccgcgcg atggcggaag gtaaactgat ttacacctgt
    47941 ggtcaagcgg tcgccatcga cggcggctta agtatgccgc gcttctgagg ctgaatcatg
    48001 agagatctgt tacaacgccc ggatttattc agtatcaata ccgcgacact gggctataaa
    48061 acgccgctac ccgccattat tgatgcctgc gccgcacgcg gtattggcgc aattgccccc
    48121 tggcgcaggg agttgcaggg tgaagatctc cagcaaatca cccgccagct tgccgccagt
    48181 aacatgagcg tttccggttt gtgccgtagc acttactaca ccgcgccgac actggcggaa
    48241 cgcaaactgg cgattgatga taaccgccgg gcgctggacg acgcagcggt gcttaacgcc
    48301 gcctgttata tgcaggtggt tggcggtctg ccaacgggca ctaaagatct atatgaagcg
    48361 cgagaacaag tgaaacaagg gattcaccag ttgctgccac attcgaaaga tgtcggcgtg
    48421 ccaattgcgc tggagccact gcacccgatg acagctgccg atcgctcttg cctgtgcacg
    48481 ttacgtcagg cgctggactg gtgtgatgaa ctcgatcccg atggtgagtt tggcctcggc
    48541 gtggcggtgg atgtttatca cgtctggtgg gatccggatc tcgccagtca gatcctgcgt
    48601 gccggaaaac gcatcctcgc gtttcatgtt tccgactggt tagtgccgac cactgatctg
    48661 gtcaatgacc gtggtatgcc gggagatggc gtcatcaata ttccgtcaat tcgtcggctg
    48721 gttgaaaacg ccggatttaa tggtgctatt gaactggaaa tattctcgcc ttactggtgg
    48781 cagaaagata ttaacagcac gctggatatt agcgtcgatc gcatcgcgca ttattgttaa
    48841 ggaatgatca tgagtgacca acccgttctg ttcagcagag cggcagcttc ctgccgcctg
    48901 acattgaacc gtgaagataa atgtcatgct attaatgaag aaatgatcga atctctcgac
    48961 cattatttaa atgaaattga aaacgacacc acattacgat tagtcgaatt aacggcaacc
    49021 ggcgataaat tcttttgtgc gggcggcgat attaaatcgt ggtcagccta ttcgccatta
    49081 gatatgggca gaaaatggat taaacgcggt aatgacgttt ttaatcgcct gcgaaactta
    49141 ccccaattaa ccgttgcgaa tctcaacggt cataccatcg gcggtggtat tgaactggcc
    49201 ttgtgctgcg atattcgtat tgcccgcccc ggcgcgaaat tctctaaccc ggaagtgatg
    49261 ctcggcatgg tgcctggctg gatgggcatt gaacgtgtgc ttaatcaggt tggtccggtg
    49321 gtcggtcgcc agatgctgat gctgggtaaa cgcctgacgg cgcaggaagc ccaggcggcg
    49381 aatttaattg atgaagtggt agaaaaagaa caggtggaaa gctggatggc gaaccagtta
    49441 gcgcagctgg aaaaatgcgg tccggtggca ctggcgcata ttaagcaact gattctggca
    49501 ctggaaaata aacatgccga ttacccgcac cagctactgg ccggattaat gtctgccact
    49561 caggattgcc agcaggcgac gcgcgccttt gccgaaaaaa gtagcgtgag cttccataat
    49621 caataaggaa attgtatgcg taagataaca accgccgagg cactggcggc gcaaattcag
    49681 gacggtgcga ctattgctat tagcggtaac ggcggcggta tggtagaagc cgaccatatt
    49741 ctggctgcta ttgaagcgcg tttcctgcaa accggacacc ctcgcgacct gacgctaatc
    49801 cactcgctgg gtattggcga tcgcgactgc accgttttgc gcacgcagag atgctcaaac
    49861 gcattattgc cgggcacttt acctggtcgc ccaaaatgca ggcgctggta aaaaataaca
    49921 cgattgaagc ctactgcttt cctggtggcg tgattcaggc actgctacgg gagatcggct
    49981 ccggacgtcc ggggcttttt acccacgttg ggctgggatc gtttgttgat ccacgcaatg
    50041 gcggcggtaa gtcgaatgaa tgcactaccg acgacctggt agaactgatt gaaatcgacg
    50101 gtgaaaccaa acttcgttat cgccctttca aagtggatta cgcgattttg cgtggcactt
    50161 atgccgatcc tcgaggcaac gtcagccttg aagaagaagc gattgatatg gatagctatt
    50221 ccatggcgct ggcagcacac aacagcggcg gcaaagtgtt cgtacaggta cgcgatgtgc
    50281 tggaagctgg cgcgattgaa ccacgccggg tcaaattacc gggcattctg gttgatggca
    50341 tcgttgaaca ccgcgaacaa ccacaaacct atcttggcgg ttacgacctg accatcagcg
    50401 gccaacatcg ccgtctgagt tctaacgacg ccattgaact ggttagtcat ccggtacgtc
    50461 gcctgattgc ccgtcgggcg gcacgcgaac tggtagctgg cgcttcaacc aactttggct
    50521 ttggtattcc tgggggtatt ccaggcgtag cgctgcgcga aggcgtgcct taccaaagtt
    50581 tatggctgaa cgtcgagcaa ggcgtgcata acggtatgat gctggatgat gcgtttttcg
    50641 gctgcgcccg caacgccgat gccattattc catcactcga tcaatttgag ttctacagtg
    50701 gcggagggat cgatatcacc ttcctcggca tgggagagat ggatcagtac ggtaacgtca
    50761 acgtatccca tctcaatggc aatctgattg gccccggcgg atttctcgaa attgcgcaaa
    50821 acgcccgtaa agtggtgttc tgcggcacgt tcgacgccaa aggtagcaag atcgatgtaa
    50881 cgccagatgg cttgtatatc gcccaatcag gtcaaatccc taaactggtt acccaggtgg
    50941 aaaaaatcac ttttagcgcc gcctacgcac agcaaagtgg tcaggaagtg ttgtatatca
    51001 ctgagcgcgc agtattccag ttaacggcag aaggcgttga attaattgaa atcgcgccgg
    51061 gtgtagagat tgagcgcgac attctgccgt tcatggcttt ccgtccaatt atcaagcacc
    51121 cacgcctgat ggaaagtagc ctgtttacgc cgatggagga tgcatgagtc agcttgatga
    51181 caccatcctt gatgcgctga cgcatgtcac tttcccaaag gggtttgtgt cagcagagcc
    51241 aacgtcaatt gtcaccgttg aggatgttga ttacccgatc tggcaagttg atgcgctggt
    51301 cgttggtagc ggcgcagcag gattacgtgc ggcagttgaa cttaaacgcc gccagcaaaa
    51361 tgtgctgatc gccaccgccg ggttatatat ggggacgtcg gcatgttctg gttccgataa
    51421 acagacactg tttaccgccg ctaccgcggg caacggcgac aacttcgcca aactggcaga
    51481 ggcactggcg agcggtgggg cgatggatca cgacaccgct tatgtcgaag cggtaggttc
    51541 tctgcacact cttggcgggt tgcaatatct tggtctggaa ttaccggaag atcgctatgg
    51601 cgcgattctt cgttatcaaa ccgaccatga cgaagccggg cgtgcaacct cgtgtgggcc
    51661 gcgtacctca aggttaatgg tgaaagtgct gttggaagaa gtacagcgcc tcgccattcc
    51721 agtgttgacc agtgcaacag tgattaaact gctgcatcag cgtgacgaaa acggcgaaga
    51781 ccgcgtggca ggggcaatcc tcgcgaccgg tcatcgcgcc cataacccat gggggctggc
    51841 aattgtgact gcgcccaatg tggtactggc aacgggcgga ccgggcgagc tttatcgcga
    51901 cagtgtttat ccacataaat gcttcggctc gctggggctg gcgctggagg aaggtctgac
    51961 gctaaccaat ctgaccgaaa gccagtttgg tataggcaca ccgcgcagca cgtttccgtg
    52021 gaatttgtcc ggcacctatg tgcaggtgat cccgtatacc tattccgtgg atgctgaggg
    52081 taacgagtat aacttcctcg cggattacta tcgcaccacc caagagctgg cttcaaacat
    52141 cttccgtaaa ggctatcagt ggccgttcca cgccacgcgg gtgatggatt ttggctccag
    52201 cttgttagat atggcagtag cgcaagagca gcaatcaggg cgtcaggtat ttatggattt
    52261 caatcgcaat cctgaagcgg tgccgggtga cctgccgttc tcattagatc gactggacga
    52321 cgacgttcgc gcgtatctgg aaaataacga cgctctggca ccatcgccca tcgaacggct
    52381 gcaacgaatg aatccgctgt ctatctcgct gtataagatg cacggttacg atctcaccac
    52441 acagcctttg cagtttgcca tgaataatca gcatatgaat ggcggcattg aagtggatat
    52501 ctggggacaa acctccctgc ccggcagttt cgccgtgggg gaagttgctg gcacacacgg
    52561 cgtcactcgc cctggtggtg cagcattgaa tgccgggcag gtttttgctg ttcgtctggc
    52621 acgttttatt ggttgcacgc aaaagcggaa tattgatgga gatatagcac agctggtagc
    52681 tcctacactg gcttctataa gagagatcat tactcaggcg cacgataacg ggaccgggat
    52741 gccgttgtcg gttgtgagag aaaaaattca ggcacgaatg tctgaccatg cgggatttat
    52801 ttgccatgcc gataaagtcc gacgcgccac tcgcgatgcc ctgctattga gcgaatttgt
    52861 ccaacggcat ggattggcta tcaaacatgt gggtgaagtt gtcgagctgt ttatgtggc
//
LOCUS       DAKISA010000016        52004 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.016, whole genome shotgun sequence.
ACCESSION   DAKISA010000016 DAKISA010000000
VERSION     DAKISA010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52004
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.016"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..849)
                     /gene="melB"
                     /locus_tag="OVW45_000999"
     CDS             complement(<1..849)
                     /gene="melB"
                     /locus_tag="OVW45_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCV5525511.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTL"
     gene            complement(952..2306)
                     /gene="melA"
                     /locus_tag="OVW45_001000"
                     /pseudo
     CDS             complement(952..2306)
                     /gene="melA"
                     /locus_tag="OVW45_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
     gene            2589..3497
                     /gene="melR"
                     /locus_tag="OVW45_001001"
     CDS             2589..3497
                     /gene="melR"
                     /locus_tag="OVW45_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCV5525512.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGNITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3696..5963
                     /gene="adiA"
                     /locus_tag="OVW45_001002"
     CDS             3696..5963
                     /gene="adiA"
                     /locus_tag="OVW45_001002"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCV5525513.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLSVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            6288..7049
                     /gene="adiY"
                     /locus_tag="OVW45_001003"
     CDS             6288..7049
                     /gene="adiY"
                     /locus_tag="OVW45_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCV5525514.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            7186..8523
                     /gene="adiC"
                     /locus_tag="OVW45_001004"
     CDS             7186..8523
                     /gene="adiC"
                     /locus_tag="OVW45_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCV5525515.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            8627..10270
                     /gene="eptA"
                     /locus_tag="OVW45_001005"
     CDS             8627..10270
                     /gene="eptA"
                     /locus_tag="OVW45_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCV5525516.1"
                     /translation="MLKRLLKSPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQQAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDVLQTCRRVSE"
     gene            10267..10935
                     /gene="basR"
                     /locus_tag="OVW45_001006"
     CDS             10267..10935
                     /gene="basR"
                     /locus_tag="OVW45_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCV5525517.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYTCDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            10936..12036
                     /gene="pmrB"
                     /locus_tag="OVW45_001007"
     CDS             10936..12036
                     /gene="pmrB"
                     /locus_tag="OVW45_001007"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCV5525518.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(12012..12101)
                     /gene="pmrR"
                     /locus_tag="OVW45_001008"
     CDS             complement(12012..12101)
                     /gene="pmrR"
                     /locus_tag="OVW45_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCV5525519.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(12213..13715)
                     /gene="proP"
                     /locus_tag="OVW45_001009"
     CDS             complement(12213..13715)
                     /gene="proP"
                     /locus_tag="OVW45_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCV5525520.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(13979..14845)
                     /locus_tag="OVW45_001010"
     CDS             complement(13979..14845)
                     /locus_tag="OVW45_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCV5525521.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSGGEHADFLSWLGKWSTADKQPVCWSVTQRWQTVALGMPRLCSAQRLVG
                     AMVEEIFSVNLA"
     gene            complement(14842..17070)
                     /gene="crfC"
                     /locus_tag="OVW45_001011"
     CDS             complement(14842..17070)
                     /gene="crfC"
                     /locus_tag="OVW45_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCV5525522.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELSKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHVNTRQLFSEVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFCAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            17472..17807
                     /locus_tag="OVW45_001012"
     CDS             17472..17807
                     /locus_tag="OVW45_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCV5525523.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            17965..18408
                     /gene="yjdN"
                     /locus_tag="OVW45_001013"
                     /pseudo
     CDS             17965..18408
                     /gene="yjdN"
                     /locus_tag="OVW45_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
     gene            18541..19329
                     /gene="phnC"
                     /locus_tag="OVW45_001014"
     CDS             18541..19329
                     /gene="phnC"
                     /locus_tag="OVW45_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCV5525524.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            19354..20370
                     /gene="phnD"
                     /locus_tag="OVW45_001015"
     CDS             19354..20370
                     /gene="phnD"
                     /locus_tag="OVW45_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCV5525525.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQGVKSNNGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            20496..21275
                     /gene="phnE"
                     /locus_tag="OVW45_001016"
     CDS             20496..21275
                     /gene="phnE"
                     /locus_tag="OVW45_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCV5525526.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            21296..22021
                     /gene="phnF"
                     /locus_tag="OVW45_001017"
     CDS             21296..22021
                     /gene="phnF"
                     /locus_tag="OVW45_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HCV5525527.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGIGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            22022..22474
                     /gene="phnG"
                     /locus_tag="OVW45_001018"
     CDS             22022..22474
                     /gene="phnG"
                     /locus_tag="OVW45_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HCV5525528.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            22471..23055
                     /gene="phnH"
                     /locus_tag="OVW45_001019"
     CDS             22471..23055
                     /gene="phnH"
                     /locus_tag="OVW45_001019"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HCV5525529.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLATPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTDTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            23055..24119
                     /gene="phnI"
                     /locus_tag="OVW45_001020"
     CDS             23055..24119
                     /gene="phnI"
                     /locus_tag="OVW45_001020"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HCV5525530.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLTKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            24112..24957
                     /gene="phnJ"
                     /locus_tag="OVW45_001021"
     CDS             24112..24957
                     /gene="phnJ"
                     /locus_tag="OVW45_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HCV5525531.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTECTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            24954..25712
                     /gene="phnK"
                     /locus_tag="OVW45_001022"
     CDS             24954..25712
                     /gene="phnK"
                     /locus_tag="OVW45_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HCV5525532.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            25823..26503
                     /gene="phnL"
                     /locus_tag="OVW45_001023"
     CDS             25823..26503
                     /gene="phnL"
                     /locus_tag="OVW45_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HCV5525533.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLIVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            26500..27636
                     /gene="phnM"
                     /locus_tag="OVW45_001024"
     CDS             26500..27636
                     /gene="phnM"
                     /locus_tag="OVW45_001024"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HCV5525534.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARKIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            27636..28193
                     /gene="phnN"
                     /locus_tag="OVW45_001025"
     CDS             27636..28193
                     /gene="phnN"
                     /locus_tag="OVW45_001025"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HCV5525535.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            28180..28614
                     /gene="phnO"
                     /locus_tag="OVW45_001026"
     CDS             28180..28614
                     /gene="phnO"
                     /locus_tag="OVW45_001026"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HCV5525536.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFSANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            28616..29374
                     /gene="phnP"
                     /locus_tag="OVW45_001027"
     CDS             28616..29374
                     /gene="phnP"
                     /locus_tag="OVW45_001027"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HCV5525537.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACDRARRSPQYRRQPCSGVV
                     KFNEAITLIDAGLHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPIYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVMDCSHPPRADAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(29521..29850)
                     /gene="yjdP"
                     /locus_tag="OVW45_001028"
     CDS             complement(29521..29850)
                     /gene="yjdP"
                     /locus_tag="OVW45_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HCV5525538.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(29919..30236)
                     /locus_tag="OVW45_001029"
     CDS             complement(29919..30236)
                     /locus_tag="OVW45_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HCV5525539.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            30489..32474
                     /gene="yjcS"
                     /locus_tag="OVW45_001030"
     CDS             30489..32474
                     /gene="yjcS"
                     /locus_tag="OVW45_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HCV5525540.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTIGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            32683..32958
                     /locus_tag="OVW45_001031"
     CDS             32683..32958
                     /locus_tag="OVW45_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525541.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            32977..34008
                     /gene="mdtN"
                     /locus_tag="OVW45_001032"
     CDS             32977..34008
                     /gene="mdtN"
                     /locus_tag="OVW45_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCV5525542.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            34008..36059
                     /gene="mdtO"
                     /locus_tag="OVW45_001033"
     CDS             34008..36059
                     /gene="mdtO"
                     /locus_tag="OVW45_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCV5525543.1"
                     /translation="MSALNSLPLPMVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGRMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVSNPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            36056..37522
                     /gene="mdtP"
                     /locus_tag="OVW45_001034"
     CDS             36056..37522
                     /gene="mdtP"
                     /locus_tag="OVW45_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCV5525544.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            37720..39867
                     /gene="fdhF"
                     /locus_tag="OVW45_001035"
     CDS             37720..39867
                     /gene="fdhF"
                     /locus_tag="OVW45_001035"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:38137..38139,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCV5525545.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            39961..40650
                     /locus_tag="OVW45_001036"
     CDS             39961..40650
                     /locus_tag="OVW45_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCV5525546.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(40729..42042)
                     /gene="gltP"
                     /locus_tag="OVW45_001037"
     CDS             complement(40729..42042)
                     /gene="gltP"
                     /locus_tag="OVW45_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCV5525547.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(42384..42980)
                     /gene="nrfG"
                     /locus_tag="OVW45_001038"
     CDS             complement(42384..42980)
                     /gene="nrfG"
                     /locus_tag="OVW45_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCV5525548.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTCAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(42977..43360)
                     /gene="nrfF"
                     /locus_tag="OVW45_001039"
     CDS             complement(42977..43360)
                     /gene="nrfF"
                     /locus_tag="OVW45_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCV5525549.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(43353..45071)
                     /locus_tag="OVW45_001040"
     CDS             complement(43353..45071)
                     /locus_tag="OVW45_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCV5525550.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWEFKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFCH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(45091..46047)
                     /gene="nrfD"
                     /locus_tag="OVW45_001041"
     CDS             complement(45091..46047)
                     /gene="nrfD"
                     /locus_tag="OVW45_001041"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCV5525551.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(46044..46715)
                     /gene="nrfC"
                     /locus_tag="OVW45_001042"
     CDS             complement(46044..46715)
                     /gene="nrfC"
                     /locus_tag="OVW45_001042"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCV5525552.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(46712..47278)
                     /gene="nrfB"
                     /locus_tag="OVW45_001043"
     CDS             complement(46712..47278)
                     /gene="nrfB"
                     /locus_tag="OVW45_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCV5525553.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(47323..48759)
                     /gene="nrfA"
                     /locus_tag="OVW45_001044"
     CDS             complement(47323..48759)
                     /gene="nrfA"
                     /locus_tag="OVW45_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCV5525554.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            49153..51111
                     /gene="acs"
                     /locus_tag="OVW45_001045"
     CDS             49153..51111
                     /gene="acs"
                     /locus_tag="OVW45_001045"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HCV5525555.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEKMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLTCGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            51311..51624
                     /locus_tag="OVW45_001046"
                     /pseudo
     CDS             51311..51624
                     /locus_tag="OVW45_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
     gene            51621..>52004
                     /gene="actP"
                     /locus_tag="OVW45_001047"
     CDS             51621..>52004
                     /gene="actP"
                     /locus_tag="OVW45_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="member of the sodium:solute symporter family;
                     cotranscribed with the acs gene which encodes acetyl
                     coenzyme A synthase; mutations affect acetate uptake;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HCV5525556.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKLVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNL"
BASE COUNT        12564 a        14342 c        13501 g        11597 t
ORIGIN      
        1 taacgtcacc aggttagcag ctcccgcata cgacagataa taggggaaca aatccgcatc
       61 accgataaca tatgagaaat aatagatagc aaatccggta ataatgttgc tggctacatt
      121 ataagcaaga gccataccca agaggcatga aagctgatcg tttttataaa ttagcgcaac
      181 gatggcttta agtgtcagat ggcttccttc agcagacggt tgattgtctg acgaaaagac
      241 ttcatgcaca ttgcgcagag tgatgatggt tgaaacaata aaaaaggcga tcagtaccag
      301 tgtgaacatc tgaaagccaa atccccgatc gccaccgccg acataattaa caaatggtag
      361 cgtcacacct gccgtaacaa agcccgccag actggcaaaa aaacgcggat aaggaaccag
      421 ttgttcacgc tcacgtttat cgagcgtgat ggttggaacc agcgaccaga agggaatatc
      481 cataatggtg taagtcatgc cccagaggat gtaggtcacg caaacaaaga caatctgagt
      541 agtaccttca aacagatgcg cactaaagag gagaaataag attacagagt ttgccaacgt
      601 accgatcagg atccagggtt tgaacttacc ccatcgcgat cgcgtagcat ttacaatcca
      661 tcccataatc ggatcgttaa tagcatccca gatcctcgcc accagaaaca aagtaccgac
      721 caaacccaca gacagcccaa cgacatcggt gtagtaatac atgaggtaca tatacacaat
      781 gccgatcgca aaatccttcc cgaacgctcc aaatccataa ctgagttttg tagtcattga
      841 aatgctcata gggtatcggg tcgctgttac caacgcccgc cttctgttat tggtatcgtc
      901 agaggtgtgc gccggaataa atcccccggc gcagtagcgt ttagtcgcgt tttaacggtg
      961 caaccagcct ggcagccagt cgccgtgggc ggcaatcagg tcgtcaacaa gagcatatat
     1021 ttcgtcaatg cccagcacgg cggcagtatg cgggtccatc atcgcggcgt ggtaaacacg
     1081 gtcgcgattt tccgtaagaa tagcttcggt cagcagcgtc tgtacattga tgttggtttg
     1141 catcagggcg gccagatgcg aaggtagcgt accgacttta gtcggctgaa tgccattagc
     1201 atcaaccaga caggctactt ccacgcaaca tccttgtggc aggttatcaa tcaaaccatc
     1261 gttacggacg ttgccgtaaa tcacactcgg ctcgccagtc cagatagcgt tcatgattgt
     1321 gctggcatat tctcgtgacg gtttaatatc aatccgggag gcgtttttat actcctccag
     1381 ctctttatgc cagttcgcca gctgctcgac gcagcgtttc gggtactcat ccagcggtac
     1441 tttataacgc tcaatcaaat cctcacgacc tggcttaata aaccacggcg tgtattcagc
     1501 aaaatgttct gacgattccg taacgaagta gcccagcttt tgaacatttc gtagcgcaca
     1561 atattctggc agcgagtatt accatgaata tttggcttcg gtgcctgccc tgcttcataa
     1621 gccgccagca gttccgggta gagattcacg taactgccgt cggcggtttt acgctccagt
     1681 tccaggtaaa acgccatatg gttgatacca gcgcaacggt aacgcagcgt ggacggatcg
     1741 atattgaggt cacgcgccag ctcttccgcc gttccctgca ccgaatggca cagccctacc
     1801 tgtttgatat gcggatagcg ggcatacatc gcccaggtat tcatcgccat tgggttaacg
     1861 tagttgagca tggtggcatc ggggcagact tccgtcatgt cctcgcaaat ttgccacaga
     1921 tgcggaatgg tacgtagcgc gcgcataata ccgcccggcc ccaacgtatc ggcaatggtt
     1981 tgttccagac catgccgctt acagacctcg aaatcagtca ccgtgcaagg ttcataaccg
     2041 ccaatctgaa atgccaccac gacaaaatcg gcatcctgta aggcttcttt ctgttgggtg
     2101 tggcaggtga ttttgccgct ggcccctgct gaatccatca gcttacgcac cacaatatgc
     2161 gactcttcca ggcgggtggg atcaatgtcc atcagggcaa tatgcgccgt tttcagcgcc
     2221 tcgcgatgga acacatcacc aagaatattt ttaacgaaaa tcgtcgaacc agcgccgata
     2281 aatgtaattt tgggtgcagt catcatgcag atctcctggc ttgcttgaat aacttcatca
     2341 tggcaggcga atctgcagaa aacctccggc ttccaggttg agcattccca taaactcaga
     2401 tcctgcgtga agcagcagta aatctgagtt tatggtaata agttacctga aaagcagagt
     2461 tttcggcctc atgatgctcg gacgctcccc tcgcctctct gggaagaggg tgaaaaccgt
     2521 gctcccactc gcagtcatcc tccctcactc ctgccataat tctgatattc caggaaagag
     2581 agccatccat gaatacagat acgtttatgt gcagcagcga cgaaaaacag acccgcagcc
     2641 cgctgtcgct gtattcggaa taccagcgga tggagattga gtttcgcgca ccgcatatca
     2701 tgcccaccag ccactggcat ggtcaagtcg aagtgaatgt gcctttcgat ggcgatgtgg
     2761 aatacctgat caacaatgaa aaagtgaata tcaatcaggg taatatcacg ctgttctggg
     2821 cctgtacacc gcaccaacta acagataccg gaacctgtca gagcatggcg atttttaatc
     2881 tgccgatgca tctgtttctc tcctggccgc tggataaaga cctgattaac cacgtcactc
     2941 acggcatggt gatcaaatca ctggccacac agcaacttag cccgtttgaa gtgcgccgct
     3001 ggcagcagga attaaacagt ccgaacgagc aaattcgcca gctcgccatt gatgaaattg
     3061 gcctgatgct caagcgattt agcctctctg gctgggaacc gattctggtc aataaaacct
     3121 cgcgcacaca caaaaacagc gtctcgcgcc atgcgcaatt ttatgttagc cagatgctgg
     3181 gctttattgc cgaaaactat gatcaggcgc tgaccatcaa cgatgtggct gagcacgtca
     3241 aacttaacgc caactatgca atggggatat tccagcgggt catgcaattg acgatgaaac
     3301 agtacattac cgcgatgcgc atcaaccacg ttcgcgcgtt actgagcgat accgataaaa
     3361 gtattctcga tattgccctg acggcaggct ttcgttcaag tagtcgtttt tacagcacgt
     3421 tcggcaaata tgtcggcatg tcgccgcaac aataccgcaa acttagccaa cagcgccgcc
     3481 agacgtttcc cggctaaaga cgatatcagt atcagccaaa aaaatagttt agccgtcgat
     3541 aataagcagt gaaaataatt taaaataatc acataacgca ttgtttataa aaataaatat
     3601 ttcaaaaatg tttgtttttc acgcgcttta cagcccgaaa aggccggaag atacttgccc
     3661 gcaacgaaga ttccttcata accgggtaag caatgatgaa agtattaatt gttgaaagcg
     3721 agtttctcca tcaagacacc tgggtcggta acgccgttga gcgtctggca gatgctttaa
     3781 gccagcaaaa tgttaccgtg attaaatcca cctcctttga tgatggtttt gccattctct
     3841 cttcaaacga agccattgac tgcctgatgt tcagctatca aatggaacat ccggacgaac
     3901 atcaaaacgt cagacaattg atcggtaagc ttcatgagcg ccaacaaaac gtgccggtct
     3961 tcctgttggg cgatcgggaa aaagccctcg ccgcaatgga tcgcgacctg ctggagcttg
     4021 tcgatgaatt cgcctggatt ctggaagata ccgccgactt tatcgccgga cgcgctgttg
     4081 ccgcgatgac ccgctaccgc cagcagctgt tgccgccact gttcagcgcg ctgatgaaat
     4141 atagcgacat ccatgaatat tcctgggcag cgccaggcca ccagggcggc gttggtttta
     4201 ccaaaacacc cgccggacgt ttctaccatg actactatgg tgaaaatctg ttccgcaccg
     4261 atatgggcat cgaacgaact tccctcggtt ctttgcttga ccatactggc gcatttggcg
     4321 aaagcgaaaa atatgccgca cgcgtatttg gtgccgatcg ctcctggtcg gtagtcgtcg
     4381 gtacttccgg ctctaaccgc accatcatgc aggcttgcat gaccgataac gatgtcgtgg
     4441 tcgttgaccg taactgccat aaatccatcg aacaaggctt gatgctgaca ggcgcgaaac
     4501 cggtctacat ggtgccaagc cgcaaccgct acggcattat cgggccaatc tatccgcagg
     4561 aaatgcaacc tgaaaccttg cagaagaaaa tcagtgaaag cccgctgact aaagacaaag
     4621 ccgggcaaaa accgtcttac tgcgtggtga ccaactgcac ctatgacggc gtgtgttata
     4681 acgctaaaga agcgcaggat ctgctggaaa aaacctccga tcgtctgcac tttgacgaag
     4741 cctggtacgg ctatgcacgt ttcaacccga tctatgccga tcactatgcc atgcgcggcg
     4801 aacctggcga tcacaacggt cctaccgttt tcgccaccca ctccacccac aaactgctga
     4861 atgcgctttc acaggcttct tatattcatg tacgtgaagg tcgtggggcg attaacttct
     4921 cccgcttcaa ccaggcctac atgatgcatg ccaccacctc cccgctgtat gccatctgcg
     4981 catccaacga cgtggcggtg tcgatgatgg acggcaacag cggcctgtca ctgacgcagg
     5041 aagtgatcga cgaagcggtt gatttccgtc aggcgatggc gcggctgtat aaagagttca
     5101 ccgctgacgg tagctggttc ttcaaaccgt ggaacaaaga agtcgtcacc gacccacaaa
     5161 ccggcaaaac ctatgacttt gctgacgcac caaccaaact gctgaccacc gttcaggact
     5221 gctgggtaat gcatccgggc gaaagctggc acggcttcaa agatattccg gataactgga
     5281 gtatgctcga cccgattaaa gtcagcatcc ttgctccggg aatgggtgaa gatggtgaac
     5341 tggaagaaac cggtgttccg gcggcgctgg tcactgcctg gcttggtcgc cacggcattg
     5401 tgcctacccg caccactgac ttccaaatta tgttcctgtt ctctatgggc gtaacccgtg
     5461 ggaaatgggg aactctggtt aacacccttt gctccttcaa acgccactat gacgccaaca
     5521 caccgctggc gcaggtgatg ccggaacttg ttgaacaata tcctgacact tacgcgaaca
     5581 tggggattca cgatctgggt gacaccatgt ttgcctggct gaaagaaaac aaccctggcg
     5641 cacggttgaa cgaagcctat tccggcctgt cggtggcgga aatcaccccg cgtgaagcgt
     5701 acaacgcgat tgtcgacaac aatgtcgaac tggtatccat tgaaaatctg ccaggacgca
     5761 tcgcggcaaa ctcagttatc ccatatccgc caggaatccc gatgctgctg tctggtgaaa
     5821 acttcggcga taaaaacagt ccgcaagtaa gttatttacg ctcgctgcaa tcctgggacc
     5881 accatttccc tggatttgaa cacgaaactg aagggactga aattattgac ggtatttacc
     5941 acgttatgtg tgtgaaagcg taaccactat tccgctgaag gcgtaattgt ttaaataaca
     6001 ttacgccgcc tggccttagg ccttttgagt atggcaacgt tttcataaaa attgctgcaa
     6061 acaaaaatgt catacttttt gcgcggcccc accccgcgct tttttttgcc tgttatttat
     6121 cctgtaaaaa aatatgtaca tgagaaatta ctataaaaat ttgtaatatt agtaaaactc
     6181 gttattttta tgcatgttta tattcatcat acaattatat aaccatttcc cggtatcgct
     6241 ttgctttagc gagaactggt cttttattcg cactcaggag tacatgtatg aggatttgca
     6301 gcgaccaacc ttgtattgtt ttattgactg aaaaagatgt ctggataagg gtgaatggga
     6361 aagaacctat tagccttaaa gctaaccata tggcgttatt aaattgtgaa aataatatta
     6421 tcgacgtctc ctctcttaac aacactttgg ttgctcatat tagtcacgac atcatcaaag
     6481 attacctccg gtttctgaat aaagatctct cgcaaatacc agtatggcaa cgtagcgcta
     6541 cgcccatact caccctgcca tgcctgacgc cagacgtctt tcgcgttgcc gcgcaacaca
     6601 gcatgatgcc cgcagaaact gagtcagaaa aggaacgaac acgcgcatta ttattcacgg
     6661 tgctatcccg ttttctcgac agtaaaaaat tcctttcatt aatgatgtat atgttacgta
     6721 attgtgtaag tgacagcgtt tatcaaatta ttgaaagcga tattcacaaa gactggaatc
     6781 ttagtatggt agccagttgt ttatgtctta gcccaagtct gttaaagaaa aagctgaaaa
     6841 gcgaaaacac cagttatagc caaataatca ccacctgccg catgcgttat gccgtaaatg
     6901 aattaatgat ggacggtaaa aatatctctc aggtatcaca gtcctgcggc tacaacagta
     6961 cgtcgtactt tatttctgtc tttaaagact tctacggtat gacgccgctg cattatgtta
     7021 gtcagcacag agaacgaact gtcgcctgat ttttaacctt aacgaagagc tatattaata
     7081 acggcatcag cgataacccg gtcgataata attcaactat cgaatgcagg cgtatgatat
     7141 gacgtaattt attgtcacga agctcgcctt cgcaggagtt taattatgtc ttcggatgct
     7201 gatgctcaca aagtgggctt aatccccgtc accctgatgg tgtcggggaa tattatgggg
     7261 tcaggtgttt ttctgttacc tgcaaacctg gcctccactg gcgggattgc catttatgga
     7321 tggttggtga cgattatcgg tgcgctgggg ctctcgatgg tatacgccaa aatgtcgttc
     7381 ctcgatccaa gtcctggtgg ttcttacgct tacgcccgcc gctgctttgg cccgtttctc
     7441 ggttatcaaa ccaacgtcct ctactggctg gcctgctgga tcggcaatat cgctatggtg
     7501 gtcattggcg taggatattt aagttacttc ttcccgattc tgaaagatcc attggtatta
     7561 accatcacct gcgtcgtggt gctgtggatc ttcgtcctgc tgaacattgt cggtccgaaa
     7621 atgatcaccc gtgtgcaggc cgttgccacc gtgctggcgc tgatccccat cgtcgggatt
     7681 gccgtatttg gctggttctg gttccgtggt gaaacctata tggcggcatg gaacgtcagc
     7741 ggcctgggca ccttcggtgc gattcaaagt acccttaacg ttacgctgtg gtcgttcatc
     7801 ggtgtggaaa gtgcctccgt tgccgcaggt gtggtgaaaa acccgaaacg caatgtccct
     7861 atcgccacca ttggtggggt attgattgcc gccgtttgct atgtgctctc caccaccgcg
     7921 attatgggga tgatccctaa tgccgcactg cgcgtttctg cctcgccatt cggtgatgcc
     7981 gcacggatgg cgttgggtga caccgctggg gccattgttt ccttctgcgc agctgcgggt
     8041 tgcttaggtt cactgggcgg ctggacgttg ctggcgggtc aaacggcgaa agccgctgcc
     8101 gatgacggac tgttcccacc gatttttgcc cgtgtaaata aagcgggtac gccagtggcg
     8161 gggctgatta tcgtcggtat tttgatgacc atcttccagc tcagtagcat ttcgccaaac
     8221 gcaaccaaag agttcggtct ggtatcttcc gtgtcggtca tctttacact ggtgccatat
     8281 ctttacacct gtgcggcgtt actgctgctc ggccacggtc actttggtaa agcacgcccg
     8341 gcatatctgg ctgttactac cattgccttc ctctactgca tctgggccgt ggtggggtcc
     8401 ggggcgaaag aggttatgtg gtcatttgtc accctgatgg tcatcaccgc catgtatgcg
     8461 ctgaattaca accggctaca taaaaacccg tatcccttag atgcaccaat aagcaaagat
     8521 taattcccct taatccagca aacataaaag ccaaccttaa gaacttaagg ttggcttaat
     8581 tttgctttgc gagcatatgc gcactttgtt cgatggaaac accgtgatgt tgaagcgcct
     8641 actaaaaagc ccctctttga atttactcgc ctggctattg ttggccgctt tttatatctc
     8701 tatctgcctg aatattgcct tttttaaaca ggtgttgcag gcgctgccgc tggattcgct
     8761 gcataacgta ctggttttct tgtcgatgcc ggtcgtcgct ttcagcgtga ttaatattgt
     8821 cctgacacta agctctttct tatggcttaa tcgaccactg gcctgcctgt ttattctggt
     8881 tggcgcggct gcacaatatt tcataatgac ttacggcatc gtcatcgacc gctcgatgat
     8941 tgccaatatt attgatacca ctccagcaga aagttatgcg ctgatgacac cgcaaatgtt
     9001 attaacgctg ggattcagcg gcgtgcttgc tgcgctgatt gcctgctggc taaaaatcaa
     9061 acctgccacc tcgcgtctgc gcagtgttct tttccgtgga gccaatattc tggtttctgt
     9121 actgctgatt ttgctggtcg ccgcactgtt ttataaagac tacgcctcgt tgttccgcaa
     9181 taacaaagag ctggtgaaat ccttaagccc ctctaacagc attgttgcca gctggtcatg
     9241 gtactcccat cagcgactgg caaatctacc gctggtgcga attggtgaag acgcccaccg
     9301 caacccgtta atgcagaacg aaaaacgtaa aaatttgacc atcctgattg tcggcgaaac
     9361 ctcgcgggcg gagaacttct ccctcaacgg ctacccgcgt gaaactaacc cgcggctggc
     9421 gaaagataac gtggtctatt tccctaatac cgcatcttgc ggcacggcaa cggcagtttc
     9481 agtaccgtgc atgttctcgg atatgccgcg tgagcattac aaagaagagc tggcacagca
     9541 ccaggaaggc gtgctggata tcattcagca agcgggcatc aacgtgctgt ggaatgacaa
     9601 cgatggcggc tgtaaaggtg cctgcgaccg cgtgcctcac cagaacgtca ccgcgctgaa
     9661 tctgcctggt cagtgcatca acggcgaatg ctatgacgaa gtgctgttcc acgggctgga
     9721 agagtacatc aataacctgc agggtgatgg cgtgattgtc ttacacacca tcggcagcca
     9781 cggtccgacc tattacaacc gctatccgcc gcagttcagg aaatttaccc caacctgcga
     9841 caccaatgag atccagacct gtaccaaaga gcaactggtg aacacttacg acaacacgct
     9901 ggtttacgtc gactatattg ttgataaagc gattaatctg ctgaaagaac atcaggataa
     9961 atttaccacc agcctggttt atctttctga ccacggtgaa tcgttaggtg aaaatggcat
    10021 ctatctgcac ggtctgcctt atgccatcgc cccggatagc caaaaacagg tgccgatgct
    10081 gctgtggctg tcggaggatt atcaaaaacg gtatcaggtt gaccagaact gcctgcaaaa
    10141 acaggcgcaa acgcaacact attcacaaga caatttattc tccacgctat tgggattaac
    10201 tggcgttgag acgaagtatt accaggctgc ggatgatgtt ctgcaaactt gcaggagagt
    10261 gagtgaatga aaattctgat tgttgaagac gatacgctgt tattgcaggg actgattctg
    10321 gcggcgcaaa ccgaaggcta cacgtgcgat ggcgtaacaa ccgcgcggat ggcggaacaa
    10381 agccttgagg ccggtcatta cagcctggtg gtactggatt tagggttacc cgacgaagat
    10441 ggactgcatt ttctcgcccg tatccggcag aaaaaataca ccctgccggt actgatcctc
    10501 accgctcgcg atacgctgac cgacaaaatc gccgggctgg atgtcggtgc cgatgactat
    10561 ctggtgaagc cttttgcgct ggaagagtta cacgcccgta tccgcgccct gctacgacgc
    10621 cataataatc agggcgaaag tgagctgatt gttggcaatc tgacgctgaa tatgggtcgc
    10681 cgtcaggtat ggatgggcgg tgaagagttg attctgacgc ccaaagaata tgctctgctg
    10741 tcacggttaa tgctcaaagc aggcagtccg gtacatcggg aaattctcta caacgacatc
    10801 tataactggg acaatgaacc ctcgaccaac accctggaag tgcatatcca caatctgcgc
    10861 gacaaagtgg gcaaagcccg tatccgcacc gtgcgcggct ttggctatat gctggtcgcg
    10921 aatgaggaaa actaattgaa tctgatgcgt tttctgcgcc gaccaatatc gctgcgccaa
    10981 cggctgatat tgaccatcgg ggccattttg ttggtgtttg agctgatcag cgtcttctgg
    11041 ctatggcatg aaagtaccga gcagattcag ctgtttgagc aggcgctacg cgacaatcgc
    11101 aacaacgatc gccacatcat gcgtgagatc cgcgaggcgg tcgccagcct gattgtcccc
    11161 ggcgtcttta tggtcagcct gacgctattt atctgctatc aggcggtacg ccgcatcacc
    11221 cgcccgctgg cggagctgca aaaagagctg gaagcgcgca ccgccgacaa cttaacgccc
    11281 attgccattc acagcgccac cctcgaaatc gaagcggtgg tttcggcgtt gaacgatctg
    11341 gtcagtcgcc tgacgagcac gctggataac gaaaggttat ttaccgctga cgtcgcgcac
    11401 gaactgcgaa cgccactggc gggggtgcgt ttgcatctgg aactgctggc gaaaacgcat
    11461 cacattgatg tagcaccgtt agtggcacgg cttgatcaga tgatggagag cgtctcccag
    11521 ctgctgcaac tggcgcgtgc cggacagtca ttttcttccg gtaattatca acatgtaaaa
    11581 ctgctggaag atgtcattct cccctcgtat gacgaactca gtaccatgct cgaccagcga
    11641 cagcaaaccc tgctattgcc agagagcgcc gccgacatca ccgttcaggg tgatgcaacc
    11701 ctgctgcgga tgttattgcg aaacctggta gaaaacgccc atcgttacag cccacaaggc
    11761 agcaacatta tgattaagct gcaagaagat ggcggagcgg tcatggccgt tgaagatgaa
    11821 ggaccgggta ttgatgagag taaatgcggg gagttgagta aagcgtttgt acgtatggac
    11881 agccgttatg gcgggattgg tctggggtta agtattgtca gccgcattac acagttgcat
    11941 cacgggcagt ttttcctgca aaaccggcaa gagacttccg gcacgcgggc ctgggtacgg
    12001 ctgaagaaag atcagtacgt ggcaaaccag atataaagaa agctgctgac caccagcaca
    12061 ctgaacacgg tagttaaact ttcataaaca cggtttttca ttgcactctc ctccgttaac
    12121 ctggaggaga gtatgcgcgt cagagattaa gtgaacctta agagttcaat tggcctgata
    12181 agacagcgtc acatcaggcc atccgtttca gcttattcat caattcgcgg atgttgctgc
    12241 accaggcggg tacgtttcgc ctgcaaatcg gcaatctcgt ggtcaatatc atcgattttc
    12301 tgctcgatat tatcgtaatg ctcgacgaga atttccttcg cttcctgtat atctgacgcc
    12361 gccggtgtcg cacctttcaa cggacgattt gccgtctctt tcatggttac gccggtgatt
    12421 aaaccaatca ccgccactac catcaggtaa taggcaggca tcatcagatt ctgcgagctt
    12481 tcgaccagcc aggccgccag tgttggcgtc agaccggcaa ccagcaccga aatattaaat
    12541 gccgccgcca gcgcgctgta acggatatgc gtcgggaaca tcgctggcaa ggtagaagcc
    12601 ataacgcccg taaagcagtt aaggatcacc gccagcatca gtaacccggc aaaaatcagg
    12661 ccgatgacgt tactgttaat cagaataaac gccgggatcg ccaacacaaa cagggcaaca
    12721 ctaccaagta gcacaaacgg acgacggcca aaacggtcac tcagcaagcc catcaccggc
    12781 tggacaaaca gcataccgat cataatggcg ataataatca gcaccccgtg gtcttcggag
    12841 taatgcaggt tatgcgacaa ataactcggc atataggtca gcaacatgta gtaagtcacg
    12901 ttggtggcaa ttaccagacc aatacatgtc aacaggctgc gccagtattt agtggcaatc
    12961 tctttaaacg agactttcgg gccatcctgc aaaccttcgc ggtcgccctg ttccagttta
    13021 tcaacatgct gctggaacgc cggagtttct tccagcgcat ggcgcaggta aagcccgata
    13081 atccctaacg gcagagcaat aaagaacgga atacgccagc cccagtcgag gaagttcgct
    13141 tcgccgacaa tggtcgaaat taacaccacc acgcccgcac ccagcacaaa cccggcaata
    13201 gaaccgaagt ccagccagct gcccataaag ccacgtttac ggtcagggga gtattccgca
    13261 acaaatatcg acgccccggt atattcaccg ccgaccgaga aaccttgtgc catcttacag
    13321 atcaacagca gaatcggtgc ccaaatacca atcgtgtcgt aggacggtat taagccaata
    13381 cagaacgtac tgatcgacat aatcacaata gtgatagcga ggatcttctg gcgaccatat
    13441 ttatcgccca acataccaaa gaagagcccg ccaagcggtc gaatcagaaa gggaacggag
    13501 aaagtggcaa gtgcagcaac catctgcacg ctggggtcag cccccgggaa aaaaacttta
    13561 cctaatgcgt aagcaacaaa accataaaca ccaaaatcga accattccat tgcattaccc
    13621 agtgatgctg cggtaatggc tttacgcagt ttaccgtcat caataatggt gacatcacga
    13681 agggtaatcg gttttacttt tttccttttc agcatagctt tcctcgcaga gttggcccat
    13741 ttccgcaaac gccagaaaaa tcagcgcgac ggccgcaaaa ggggagcggg aattgcagga
    13801 tgcaatctct gtaacaagcg tagcgggttt gcttacaccc tccggttaag gaatgtacaa
    13861 tcaaaccagt tgacgcctga attgggcagt taatgacctt tttaccctac cagtgtttaa
    13921 atttgtgatc aacttcacac atggatttcg tttaggactc attgatgtaa ctgattattt
    13981 acgccaaatt tacagagaag atttcctcga ccattgcccc caccaaacgc tgcgctgaac
    14041 agagtcgtgg catccccagc gcgactgttt gccagcgttg ggtcactgac cagcaaacgg
    14101 gctgtttatc tgccgtggac cactttccca gccaacttaa aaaatcagca tgctcccctc
    14161 ccgaagaggc aaaccactga tggtaatagt gacgcgtggt cggtgccgca ttgatactct
    14221 ctgccagatg ttgtgtcgct ttctcgcgca aatcacccag taacaacacc accgcttgcg
    14281 ggcttcccag acggcaggca tcgccaaatg caccccagcg caactcctcc agaacgataa
    14341 aacagcgccc aggcaacgac caggcgttat acgccccagc acgccagcgg gtaaaaattt
    14401 gctcgctctg atcacctgcc tgcaccagta ggcctcgttg cgttaacgcc ttttccttcc
    14461 agcgacaacg ggctgaaaac cactcataca aggcggcaat ttgctggggg agctccggga
    14521 aagcagagtg ctggcggtga tccatttttt gcagcagcgc cagatgctgc gcgatctgcg
    14581 tcagcgccag atgaccggga tccagcatcg acgccagttt ttccaccatt gtcagtcgca
    14641 ggctaagcgg cgcatttaac atgccgccgt tcgtccatgt ctgaagctgc acacgcgaaa
    14701 gaagttcctg catcagtcgt tcacgaaact gctgctgatg ggcttgtgga aggcgggcat
    14761 catcgccctg cgccagatcc actaaaaagc ggggataaac cgactccagc acgcgaccgg
    14821 ggccgtccag taacgtcttg gtcaatatcg ttctgccgtg aaaagtgttt gaatatcatc
    14881 gcgtaacagc tgggcgtcgg tgtaaatcca gccgtgagtc atcacagtct gctgcaattg
    14941 ctgctgcatc agcctgacca ccgattcatt ttgttgacgc agagccaggc tttcgcgtaa
    15001 acgcgtctgt aattccgtca aacatgaagc gaactcagcg caaaaagtat tcatgcctgc
    15061 cgtaacagat tcatcgacct gctctgccag aactttagcc atttgttggc aataaagatc
    15121 gacttctgcg cttaaagctc gttgcaacac actgtaatca accgtctctg tcggggattt
    15181 ctcatttccc cgtccccagt cgggctgatt caaccagcgc gaaaaagtct cacgcacaac
    15241 gcctaaacgc gtgctctgct cgtccgttgc atcctggcgc gaaatgactt cactgaacag
    15301 ctggcgagtg ttgacgtggg gaactactcc gtgaaaaaca ggaaaatgaa acccaggacg
    15361 aaaccctgac tcgctcaatt ccattttgac ttgttgctca atgggccgaa taacatcggt
    15421 taacacccgg caaagggtgg attcaagctc ggcaaaacgc agcgtaaagt cgcgactgat
    15481 ggtgttctgc gccgtctgta acagtgtctc acagcaggta cgaatctcgc ttaacggctc
    15541 cgaatcatcc tgaaacaagg cggctaactg cgcattcagc gcatcttgct gttgacgcag
    15601 aaagtggttg gcggaggtca gggccagctc gatttcatgt ttaatctcgc cgctcacctg
    15661 cgcctgattg agttgcaata gctgcaaact ttcttcgacc tgatggatat tttgccgcaa
    15721 ttgttcacaa gcgacgttta acccgtgcgc acgaaaatcc aggtattccc gcgcctgctg
    15781 cgcgtaattc aacagtttat gcgcagcaga tcgcagagca tacaacgagg cgttagcgta
    15841 agcggcatga agcaacgcct gaattggctg ggcgaacagc gaatcttccc acaactgatc
    15901 ggcagcatga cgaatatgtt cgaggtccgc cagatcggca tgacgccagc gcctgccgag
    15961 cgcggcatgg gcaaaatctt ccacccagcg ttgttgctct ggcgctggta acttaccgtt
    16021 gttggctaac tcatggcgcg ctcgatttgc caggtagccc cacatcgacg acaccggaaa
    16081 tatctgctgt ggcgtaatac agcctttcat cagcgtcccg gaaatcagtg cccgcacctg
    16141 gtcggcgtcg tcactgttac gatcctgttg atcgaactta ttgaccagca catacagtgg
    16201 caccgattgc cccaccgcca aaatcgcctc acggacctct tcatcggaga tcgatttcag
    16261 ttgcgtataa tccagcaccg ccagtaccgc cgaggcgcgt gccagctgct ggttaagcat
    16321 tttttgcaga tgcggttgcc cggcttcatt tggcccgggg gtatccagta acgtcaactg
    16381 accgggataa ctctccagcc ccgccagatg gacaaactcc acttcaatca cgggaatatg
    16441 ctcaatggcg gcgtaagcag aaaaaggaaa atcgacgtcc agcgccttcg ccagtcgcac
    16501 taaatcattc aaacttttca gacaatgaaa aataggctgg gcacccagat aatatttttc
    16561 gaaagcgacg ccattttcga tccgctgcat aagcgcacgc atatctttat ctatttccag
    16621 cacatcggtc agatgcttaa tatcgcaatc acgcaggcgc tgttgtaatt gttgaattaa
    16681 acaatcgatt ggcgcgacat gtgaaaaatg cagtaccggt tccttttgcc cgggcgtatg
    16741 gcgaataagc gtcggcagcg cagtcattgg gcgattacga ttaggcagaa cctccgtacc
    16801 aacaatggca ttaatggtgg ttgatttccc tgctttcata gtaccgacaa tcgccagcac
    16861 catttccagt cgggaaattt tactcaactc attattcagc atcgcgtgac gttcggcgat
    16921 attaggctga ctccagggta aagccagttg tggcgcgtcg tctccgggta cagagagagg
    16981 cattttttcc agtaactgca actgttggcg agaaagctgt aacaggcgtt cagcctcctg
    17041 acttaactca tacagggtct gtgtgtacat agaaaattct tccttaaagc aaattttgtt
    17101 acttttattt agccagattg tttttgagtt ctgtttttgg cttttataat tactgcaaga
    17161 aataatttta tatttagtgt gttgtttttt atcagaataa ataacgtctt ctgatgcgtt
    17221 taaaacgtca gaaagataaa aatatcatgt gaataaaaaa aagaacaagt agagcattaa
    17281 cattatctta aataataaat agaggcaaaa agattatttt ctttttgcgt ttcctttcaa
    17341 atgaaaacga tcgtcgtcta aaatcagcag tacccccgac aaactcaggg attttgtgta
    17401 taattgcggc ctttttcggc aatctgccgt tttttggcgc ttttgccctg ctgacttttg
    17461 aggaaatcca catgtcatta ccacactgcc caaaatgcaa ctccgaatac acttacgaag
    17521 ataacggcat gtacatctgc ccggaatgtg cctacgaatg gaacgacgca gaacctgcac
    17581 aggaaagcga cgagctgatc gttaaagatg ctaacggcaa tctgctggct gacggcgaca
    17641 gcgttaccat cattaaagat ctgaaggtga aaggtagctc ttcgatgctg aaaattggca
    17701 ccaaagtgaa aaacatccgc ctggttgaag gcgaccataa catcgattgc aaaatcgacg
    17761 gttttggtcc gatgaaactg aaatctgagt ttgtgaaaaa gaactgattg tattgtgatc
    17821 ggtaagccgg ataaggcgct cgcgccgcat ccggcaacga tgccgactgc ctgatgcgac
    17881 gcttgcgcgt cttatcaggc agcctctcat ctgtataaat ttcgaactac acttaattgg
    17941 cttctcttaa ctgaggtcac catcatgccg ttaagtccct acctctcttt tgccggtaac
    18001 tgtgccgacg caattgctta ttatcaacgt acgttgggcg ctgaactgct ctataaaatc
    18061 agcttcggcg aaatgccaaa atcagcgcag gacagcgccg agaactgccc ttccggaatg
    18121 caatttcccg ataccgccat cgcccacgcc aatgtgcgca ttgccggaag cgacatcatg
    18181 atgagcgatg ccatgccgtc aggaaaagcc agctactccg gctttacgct ggtgctcgat
    18241 tcgcaacagg tcgaagaagg aaaacgctgg tttgacaatc ttgccgctaa cggaaaaatc
    18301 gaaatggcct ggcaggaaac tttctaggcg catggctttg gcaaagtcac cgataaattt
    18361 ggcgtaccgt ggatgattaa tgtcgtcaaa caacaaccaa cgcaataacc cgccgggagg
    18421 cccgccctcc cgcactgtca tcgaattccc gttaactctt catctgttag tcacttttaa
    18481 ttaaccaaat cgtcacaata atccgccacg atggagccac ttttttaggg aggctgcatc
    18541 atgcaaacga ttatccgtgt cgagaagctc gccaaaacct tcaatcagca tcaggcgctg
    18601 catgcggttg atctgaacat tcatcacggt gaaatggtgg ctctgcttgg gccgtcgggt
    18661 tccggcaaat ccaccctttt acgtcactta agcggtttga ttaccggcga taaatccgcc
    18721 ggcagccata tcgagctgct gggccgcaca gtccagcgcg aaggccgtct ggcgcgcgat
    18781 atccgcaaaa gccgcgccaa caccggctac atcttccaac aattcaacct ggtgaaccgc
    18841 ctgagcgtac tggagaacgt gctgattggc gcgctcggca gcacgccgtt ctggcgcacc
    18901 tgttttagct ggtttacccg cgagcagaaa caacgcgcgt tacaggcgct gacccgcgtt
    18961 ggcatggtgc attttgccca tcaacgcgtt tccaccctct ccggcggaca gcagcagcgt
    19021 gtggcgattg cccgcgcgct gatgcagcag gcgaaggtga ttctggccga tgaacccatc
    19081 gcctcgctgg acccggaatc cgcccgcatc gtgatggaca ccctgcgcga catcaatcag
    19141 aacgacggca tcaccgtggt cgtcacgctg catcaggtgg attacgccct gcgctactgc
    19201 gaacgcatcg tcgccctgcg ccaggggcac gttttctacg acggcagcag ccaacagttt
    19261 gataacgaac gttttgacca tctctaccgc agcattaatc gcatcgaaga gaacgcgaaa
    19321 gctgcctgac atccccatca ttgaggaaaa cgaatgaacg ctaagataat tgcctcgctg
    19381 gccttcacca gcatgttcag cctcagcacc ctgttaagcc cggcacacgc cgaagagcag
    19441 gaaaaggcgc tgaatttcgg cattatttca acggaatcac agcaaaacct gaaaccgcaa
    19501 tggacgccat tcttacagga tatggagaag aagctgggcg tgaaggtgaa cgccttcttt
    19561 gccccagact acgcaggcat tatccaggga atgcgcttca ataaagtgga tatcgcctgg
    19621 tacggcaacc tgtcggcaat ggaagcggtg gatcgcgcca acggccaggt cttcgcccag
    19681 acggtcgcgg cggatggatc gccaggttac tggagcgtgt tgatcgtcaa caaagatagt
    19741 ccgatcaaca acctgaacga tctgctggcg aagcggaaag atctcacctt cggcaatggc
    19801 gatcctaact ccacctctgg cttcctcgtc cccggttact acgtctttgc caaaaacaat
    19861 atctccgcca gcgactttaa gcgcaccgtc aacgccgggc atgaaaccaa cgcgctggcc
    19921 gtcgccaaca agcaggtgga tgttgccacc aacaacaccg aaaacctcga caagctgaaa
    19981 acctccgcgc cagagaagct gaaagaactg aaggtgatct ggaagtcgcc gctgatccca
    20041 ggcgatccga tcgtctggcg caagaatctc tccgaaacca ccaaagacaa gatctacgac
    20101 ttctttatga actacggaaa aacgccggaa gagaaagcgg tgctggaacg cctgggctgg
    20161 gcaccgtttc gcgcctccag cgacctgcaa ctggtgccga ttcgccagct cgcactgttt
    20221 aaagagattc agggcgtaaa aagcaataac ggactgaatg agcaggacaa gctggcgaaa
    20281 accaccgaga ttcaggcgca gctggatgac ctggaccgcc tgaacaacgc gttaagcgcg
    20341 atgagttcgg tgagtaaagc ggtgcagtaa tgtgctcgat cggtcccctc tcccttgagg
    20401 gagagggtta gggtgagggg gaaatgcgca cgctgatgcc ctcaccccgg ccctctccca
    20461 cagggagagg gagaaaacat taaacggagc caaacatgca aaccatcacc atcgccccac
    20521 ccaagcgcag ctggttctcg cttctgagct gggccattgt tctcgccgtg ttggtcgtct
    20581 cgtggcaggg cgcggaaatg gccccgctta cgctgatcaa agacggcggc aacatggcga
    20641 ccttcgccgc cgacttcttc ccgcccgatt tcagccagtg gcaggattac ctcaccgaaa
    20701 tggccgtcac gctgcaaatc gccgtctggg gcaccgcgct ggcagtggtt ctctccatcc
    20761 ccttcggcct gatgagcgcc gaaaacctgg tgccgtggtg ggtttaccag cccgttcgcc
    20821 gcctgatgga cgcctgccgc gccattaacg aaatggtctt cgccatgctg ttcgtggtcg
    20881 ccgtcggtct cggaccgttc gctggcgtgc tggcgctatt tattcacacc accggcgtgc
    20941 tctcgaaact gctttccgaa gcggtggaag cgattgaacc cggcccggtg gaaggcattc
    21001 gcgccaccgg cgccaacaag ctggaagaga tcctttacgg cgtgctgcca caggtaatgc
    21061 cgctgctgat ctcctactcc ctctatcgct tcgaatccaa cgtccgctcg gcgaccgtcg
    21121 tcggcatggt cggtgcaggc gggatcggcg tcaccctgtg ggaagcgatt cgcggtttcc
    21181 agttccaaca aacctgcgcc ctgatggtgc ttatcatcgt cacggtcagc ctgctggatt
    21241 tcctctctca acggttgcgt aagcacttta tctgataagt gaggcattga tatctatgca
    21301 cttgtctaca catccgacca gctacccaac acgctatcag gagatagccg caaaacttga
    21361 gcaggagctt cgtcaacact atcgctgcgg cgactatctt cccgccgagc agcaactggc
    21421 ggcgcgcttt gaggtaaacc gccacaccct gcgccgcgcc atcgaccaac tggtggaaaa
    21481 aggctgggta cagcgccgtc agggcatcgg cgtgctggtg ctgatgcgcc cgttcgatta
    21541 cccgctcaac gcccaggcgc gttttagcca gaacctgctg gaccagggca gccatcccac
    21601 cagcgaaaaa ctgctgtcgg tattgcgccc cgcgtccggt cacgttgctg acgcgctggg
    21661 gattaccgag ggtgagaacg ttatccattt gcgcaccctg cgtcgcgtca acggcgtcgc
    21721 gctctgttta atcgaccact actttgcgga cctcaccctc tggccgacac tgcaacgttt
    21781 cgacagcggt tcgctgcacg attttctgcg cgagcaaacc ggaattgcgc tgcgccgcag
    21841 ccagacgcgg atcagcgccc gccgcgccca ggccaaagag tgccagcgcc ttgaaatccc
    21901 gaatatgtcg ccgctgctgt gcgtgcgcac ccttaaccac cgtgacggtg aaagcagccc
    21961 ggcggagtac tccgtcagcc tgacgcgcgc cgacatgatt gaattcacta tggagcactg
    22021 aatgcacgca gataccgcga cccgccagca ctggatgtcc gtactggcgc acagccaacc
    22081 ggctgaactg gcagcccgcc tgaacgcgct gaacatcacc gccgactatg aagtgatccg
    22141 cactgccgaa actggcctgg tgcagattca ggcgcgaatg ggcggtaccg gcgaacgctt
    22201 ttttgccggc gacgccacgc tgacccgtgc cgccgtacgc ctgactgacg gcaccctcgg
    22261 ctacagctgg gtgctggggc gtgataaaca gcacgccgaa cgctgcgcgc tgattgatgc
    22321 gctgatgcag caatctcgcc actttcaaaa cttatcagaa acccttattg ccccgctgga
    22381 cgctgaccgt atggcacgca ttgccgcacg ccaggccgaa gtgaacgcca gccgggtcga
    22441 cttctttacg atggttcgcg gagacaacgc atgaccctgg aaaccgcttt tatgcttccc
    22501 gtgcaggatg cccagcacag ttttcgtcgc ctgttaaagg ccatgagcga gccgggcgtg
    22561 attgtcgccc tgcatcagct caaacgcggc tggcagccgc tgaatatcgc caccaccagc
    22621 gtgctgctga cgctggccga taacgacacg ccggtgtggc ttgccacgcc attaagtaac
    22681 gatatcgtca atcagagcct gcgttttcat accaacgcgc cgctggtcag ccagccggaa
    22741 caggcgacct tcgcggtgac ggatgaggcg atttccagcg aacagctcaa cgccctttcc
    22801 accgacaccg ccgttgcgcc ggaagcgggc gcgacgctga ttttacaggt cgccagcctg
    22861 agcggcggac gcatgttgcg tctcaccggc gcgggcattg ccgaagaacg aatgatcgct
    22921 ccgcagctgc cagagtgcat tctgcacgaa ctcaccgagc gcccgcaccc gttcccgctc
    22981 ggcatcgacc taatcctgac ctgcggcgag cgcctgctgg ctatcccgcg aaccactcat
    23041 gtggaggtgt gctgatgtac gttgccgtga aagggggcga aaaggcgatc gacgccgccc
    23101 acgccctgca agagagccga cgccggggcg ataccgattt gcccgaactg agcgtcgccc
    23161 agattgaaca gcagcttaac ctcgcggtgg atcgcgtgat gacagaaggc ggcattgccg
    23221 accgcgaact ggcggcgctg gcgctgaaac aggccagcgg cgataacgtc gaagcgattt
    23281 tcctgctacg cgcctaccgc accacgctga cgaaactggc ggtaagcgag ccgctcgaca
    23341 ccacggagat gcgtctcgaa cggcgtattt cagcggttta taaagatatt cccggcggcc
    23401 agctgcttgg cccaacctac gactacaccc atcgcctgct cgatttcact ctgctggcaa
    23461 acggcgaagc gccgacgctg accaccgccg acagcgaaca acagccgtcg ccgcacgttt
    23521 tcagcctgct ggcgcgtcag gggctggcga agtttgaaga ggatagcgga gcacagccgg
    23581 atgacatcac ccgcacgccg ccggtttacc cctgttcacg ttcttcccgt ttgcagcagt
    23641 taatgcgcgg cgacgaaggc tatttgctgg cgctggctta ctccacccag cgcggttacg
    23701 ggcgcaacca cccgttcgcg ggcgagatcc gcagtggtta catcgacgtg tcgattgtgc
    23761 cggaagagct gggatttgcg gtaaacgtcg gcgaactgct gatgaccgag tgtgaaatgg
    23821 tcaacggttt tatcgacccg ccggatgagc cgccgcactt cacgcgcggc tacgggctgg
    23881 tattcggcat gagcgagcgc aaagcgatgg caatggcgct ggttgaccgt gcgttgcaag
    23941 ccccggaata cggcgagcac gcgacaggcc cggcgcagga tgaagagttc gtgctggcgc
    24001 atgccgacaa cgtcgaagcc gcaggctttg tctcgcacct caaactcccg cactacgtcg
    24061 atttccaggc cgaactggag ctactcaaac gtctgcaaca ggagcagaac catggctaat
    24121 ctgagcggct acaactttgc ctacctcgac gagcagacca aacgcatgat ccgccgcgcc
    24181 atcttaaaag cggtggcgat ccccggttat caggtaccgt ttggcgggcg cgagatgccg
    24241 atgccgtacg gctggggaac tggtggtatc cagctcaccg ccagcgtgat tggcgaaagc
    24301 gacgtgttga aggtgattga ccagggtgcg gatgacacca ccaacgccgt gtcgattcgc
    24361 aacttcttta agcgcgtgac cggggtaaac accactgaat gtacggacga tgcgacgctt
    24421 atccagacgc gtcaccgcat ccccgaaacg ccgctgaccg aagatcagat cattatcttc
    24481 caggtgccaa tcccggaacc gctgcgcttt atcgagccgc gcgaaacgga gacccgcact
    24541 atgcacgcgc tggaggagta cggcgtgatg caggtgaaac tgtatgaaga tatcgcccgc
    24601 ttcggtcata tcgccaccac ctatgcttat ccggtgaagg tgaacgggcg ctacgtgatg
    24661 gacccgtcgc cgatcccgaa attcgataac ccgaaaatgg acatgatgcc cgccctgcag
    24721 ctgttcggcg cagggcgcga gaagcgcatc tatgcggtgc cgccgtttac ccgcgtggaa
    24781 agtctcgatt tcgacgatca cccgttcacc gttcagcagt gggatgagcc atgcgccatc
    24841 tgcggatcga cccacagcta tcttgatgaa gtggtgctgg atgacgccgg aaaccgcatg
    24901 tttgtctgct ccgataccga ttattgccgc caacagagcg aggcgaaaag ccaatgaatc
    24961 aaccgttact ttcggtcaat aacctgaccc acctttacgc gccgggcaaa ggctttagcg
    25021 atgtctcttt tgatttatgg ccgggggaag tgctgggcat tgtcggggaa tccggctccg
    25081 ggaagaccac gctgctgaag tcgatctccg cgcgtctgac gccgcagcag ggggagattc
    25141 actatgaaaa ccgttccctc tatgcaatga gcgaggccga ccgccgtcgc ctgctgcgca
    25201 ccgaatgggg cgtggtgcat cagcatccgc tcgacggcct gcgccgccag gtgtcggcag
    25261 gcggcaatat cggcgagcgg ctgatggcga ccggggcgcg acattacggc gatattcgcg
    25321 ctaccgcgca gaagtggcta gaagaggtgg agattcccgc caaccgtatc gacgacctgc
    25381 cgaccacctt ttccggcggt atgcagcagc gtttgcagat tgcccgcaac ctggtgacgc
    25441 atccgaagct ggtgtttatg gatgaaccga ccggcgggct ggatgtgtcg gtgcaggccc
    25501 gcctgctcga cctgctgcgc ggactggtgg tggagctgaa cctcgcggta gtgattgtca
    25561 cccacgattt aggcgtcgcc cgcctgctgg cggaccgttt gctggtgatg aagcaggggc
    25621 aagtggtgga gagtgggtta accgaccgcg tgctcgacga cccgcatcat ccgtatacac
    25681 agctgctggt gtcatcggtt ttgcagaatt gagccggtgc cggatgcggc gtaaacgcct
    25741 tatccggcct acaaatgcgc tccccgtagg tcggataaga cgcgtcagcg tcgcatccga
    25801 cacccgaacc acgaggcgaa aaatgattaa cgtacaaaac gtcagtaaaa ccttcatcct
    25861 gcaccagcaa aacggcgtgc gcctgcccgt cctcaaccgc gcctcgctca tcgtcaacgc
    25921 gggcgaatgc gtggtgctcc acggccattc cggcagcgga aaatcaaccc tgctacgctc
    25981 gctgtacgcc aactatctgc ccgacgaagg gcaaatccag atcaaacacg gtgacgaatg
    26041 ggtcgatctg gtcaccgcgc cagcgcgcaa agtggtggag atccgcaaaa ccaccgttgg
    26101 ctgggtgagc cagtttctgc gcgtcatccc gcgtatttcg gcgctggaag tggtgatgca
    26161 gccgctactc gataccggcg taccgcgtga agcctgcgcc gctaaagccg cgcgtctgct
    26221 cacccgcctg aacgtgccgg aacgcctgtg gcatctggcg ccatcgacct tctccggcgg
    26281 cgaacagcag cgcgtcaaca tcgcccgcgg ctttatcgtc gactacccca ttctgctgct
    26341 cgacgaacct accgcctcgc tggacgccaa aaacagcgcc gccgtagtcg agctgattca
    26401 cgaagccaaa gcccgtggcg cggccatcgt cggcatcttc cacgacgaag ccgtacgtaa
    26461 tgacgttgcc gaccgcctgc acccaatgga agcctcttca tgattatcaa taacgtcaaa
    26521 ttggtgctgg aaaacgaagt ggtacacggt tcactggagg tgcaggacgg cgaaatccgc
    26581 gcctttgccg aaagccagag ccgcctgccg gaggcgatgg acggcgaagg cggctggctg
    26641 ctgccggggc tgattgagct gcataccgat aatctggata aattcttcac cccgcgcccg
    26701 aaagttgact ggcctgccca ctcggcgatg agcagccacg acgcgctgat ggtggcgagc
    26761 ggcatcacca ccgtactgga tgccgtggca attggcgacg tgcgcgacgg cggcgatcgg
    26821 ctggagaatc tggagaagat gatcaacgcc atcgaagaga cgcagaaacg cggcgtcaac
    26881 cgcgccgagc accgtctgca tctgcgctgc gaactgccgc atcacaccac gctgccgctg
    26941 tttgaaaaac tggtgcagcg cgagccggtg acgctggtgt cgctgatgga ccactcgccg
    27001 ggccagcgcc agttcgccaa tcgcgagaag tatcgcgaat attatcaggg caaatactcc
    27061 ctcactgatg cgcagatgca gcagtacgaa gaagagcaac tggcgctcgc cgcacgctgg
    27121 tcgcagccga atcgcgaatc catcgccgcc ctgtgccgcg cgcgaaaaat tgcgcttgcc
    27181 agccacgatg acgccaccca cgcccacgtt gctgaatctc accagcttgg cagcgtgatc
    27241 gccgaatttc ccaccacgtt cgaagcggcg gaagcctcgc gcaagcatgg catgaacgtg
    27301 ctgatgggcg cgccgaatat tgtgcgcggc ggctcgcact ccggcaacgt ggcggccagt
    27361 gaactggcgc agcttggcct gctggatatc ctctcttccg actactaccc cgccagcctg
    27421 ctcgatgcgg catttcgcgt cgccgatgac gagagcaacc gctttacgct gccgcaggcg
    27481 gtgaagctgg tgactaaaaa tccagcgcag gcgcttaatc tccaggatcg cggggtgatt
    27541 ggcgagggca aacgcgcgga cttggtgctg gcgcatcgca agggcaatca cattcatatc
    27601 gaccacgtct ggcgtcaggg taaaagggtg ttctgatgat gggaaaactg atttggttaa
    27661 tggggccgtc cggctccggg aaagatagcc tgctggcgga actccgcctg cgggaacaaa
    27721 ctcagctact ggtggcgcat cgctacatca cgcgcgacgc cagcgccgga agcgaaaacc
    27781 atatcgctct gagcgagcag gagtttttta cccgcgcggg gcagaaccta ctggccttaa
    27841 gctggcacgc taacggtctg tattatggcg tcggcgtcga gatcgacctc tggctgcacg
    27901 ccggattcga cgtgctggtc aacggctcac gcgcccatct gccgcaggcg cgggcgcgct
    27961 atcaatcggc gctgctgccc gtctgtttac aggtttcgcc ggagatcctc cgccagcgcc
    28021 tggaaaaccg tggccgtgaa aacgccagtg aaattaacgc ccgcctggcg cgcgccgccc
    28081 gctatactcc acaggattgc catacgctca acaatgacgg cagcctgcgc cagtcggtcg
    28141 acacgctgct gacgctgatt catcagaagg agaaacacca tgcctgcttg tgagcttcgc
    28201 ccggccacgc agtatgatac cgacgcggtt tacgcgctga tttgtgagct aaaacaggcg
    28261 gagtttgacc accacgcatt tcgcgtgggc tttagcgcca atctgcgcga cccgaacatg
    28321 cgctatcatc tggcgctgct ggatggcgag gttgtcggca tgatcggcct gcatttgcag
    28381 tttcatctgc atcatgtcaa ctggatcggc gaaattcagg aactggtagt gatgccgcag
    28441 gcgcgcggcc tgaacgtcgg tagcaagttg ctggcgtggg cagaagaaga agcccgccag
    28501 gccggggccg aaatgaccga gctttcgacc aacgtgaagc gccacgacgc gcaccgtttc
    28561 tatctgcgag aaggctacga gcagagtcac ttccgcttca ccaaggcgct gtaacatgag
    28621 tctgaccctc acgctcaccg gcaccggcgg cgcacagggc gttccggcat ggggctgcga
    28681 gtgcgcggcc tgcgaccgag cgcggcgctc gccgcagtat cgccgccaac cgtgcagcgg
    28741 cgtagtgaag tttaacgagg cgatcaccct gatcgacgcc gggctgcacg atctcaccga
    28801 tcgctggtct cccggatcgt tccagcagtt tttgctgacg cattatcata tggatcacgt
    28861 ccaggggctg tttccgctgc gctggggcgt gggcgatccg atcccgatct acggcccgcc
    28921 ggatgaacag ggctgcgacg atctgtttaa acatcccggg ctgcttgatt tcagccacac
    28981 ggtggaaccg tttgtggtgt ttgatttgca ggggttgcag gtcacgccgc tgccgcttaa
    29041 ccactcaaaa ctcactttcg gctatttact ggaaacggcg cacagccggg tggcgtggct
    29101 gtctgacacc gctggcttgc cggaaaaaac gctgaaattt ttacgcaata atcagccgca
    29161 ggtaatggtg atggattgca gtcacccgcc gcgcgcggat gcaccgcgta atcactgtga
    29221 tttaaatacc gtgcttgcgc tgaatcaggt tatccgctcg ccacgggtga ttctgaccca
    29281 tatcagccac cagtttgatg cgtggctgat ggaaaacgca ctaccgtcag ggtttgaggt
    29341 ggggtttgat gggatggaga ttggggtggc gtgatgagag ggaatgtgcg cgctggcccc
    29401 ctcaccctaa ccctctcccc agaggggcga ggggaccgat tgtgctcgat attgaatatt
    29461 gcgctcgttt tctccctctc cccattgggg tgaggggcga tgcctgctcc atacccaacc
    29521 tcatcgccca tactcatctt ccattctccg ctcttcatcc tccagttgcc gacgctcctg
    29581 atcaagctgg cgctggcgat cgtccagctg cctgcggcga tcttcaaact ggcggcggcg
    29641 gtcgtcatat tgtctgcgcc gatcgtcgct cacttcacgc tgccagccgt cgtcgcgcga
    29701 atcttcatag tctcgcccac ggtcagggtt ataagcgtca ttaatcgcct gctgaatatt
    29761 accaatagta tcgtcgataa tatcagcctg ggccggaacg gtggacagcg tgagcagggt
    29821 gaataaaaga aatagcggaa agcgtttcat tagccaacct caaaaagaaa ctctatccac
    29881 attagtcatt actcatccat gcaagtagtg gatgaatctc aatttctccg ctgctctatt
    29941 gccgtaatcg cctccacgcg ttgttgatga cgaccgcctt cgtactgtgc gcccagccac
    30001 gcatccacaa tcatttttgc cagttcgagg ccaaccactc gtgaaccaaa agccagtgca
    30061 acttcactgg cataatgcgg ataatcagtg cgctctgacg acctggtgtc tttatcaatc
    30121 acctcactca ttgaccatga ataaaaggct ggcacagcaa cgcagaatcc tgcggtgcca
    30181 gcgacatgac cgcccattgc tccggtacgt tttttaaaaa gaaacaccgg ttgcattgat
    30241 aaattgtgct cagtatttgc cgaagtacaa tatatataat aaatatataa aacacctcat
    30301 attatttaca gagataataa attcgcgtaa ttttccctct tcagcacaaa tgcctaataa
    30361 aatggatcgt tcagctcaca cttccactta ttaaagtcgc aaattcccaa ttatgataac
    30421 cccagcttta aatgacgtat tttgcatgga ttacatattt acgacataaa tgataaatgg
    30481 agatttacat gaataactct cggttattcc gtttgagcag aattgttatt gcgttaactg
    30541 ccgccagcgg catgatggta aataccgcta acgcgaaaga ggaagcgaaa gccgccactc
    30601 aatataccca acaggttaat cagaattacg ccaaatcatt accgtttagc gatcgtcagg
    30661 attttgacga cgctcagcgt ggatttatcg ctccgctgct ggatgaaggt attctgcgcg
    30721 atgctaatgg caaaccatat tatcgcgggg aagattataa atttgatatc aatgcgcccg
    30781 cgcccgaaac cgttaaccct agcctgtggc gtcagtcgca gttaaacggt atttccggcc
    30841 tgtttaaagt caccgacaga atgtaccagg ttcgcggtca ggatatctcc aacatcacct
    30901 ttatcgaagg tgataccggg attattgtca tcgacccgct ggtgacgcca ccgagcgcaa
    30961 aagccgccct tgacctttac ttccaaaatc gcccgcaaaa accgattgtt gcggttattt
    31021 atacccacag ccataccgac cactatggcg gcgtgaaagg cattatctct gaagctgatg
    31081 tgaaatccgg taaagtgcag gttatcgccc ctgctggctt tatggacgaa gccatcagcg
    31141 aaaacgtact ggcgggtaat attatgagcc gccgtgcact ttactcctac ggcctgctgc
    31201 tggcgcataa ccctcagggt aacatcggca atggtcttgg cgtaacgctg gcatcgggct
    31261 acccgagcat catcgcaccg aacaaaacca tcaccaaaac cggtgagaag atgattatcg
    31321 acggcctgga gtttgacttc ctgatgaccc caggtagcga agcaccagcc gaaatgcact
    31381 tctatattcc ggccctgaaa gcgctgtgta ccgccgagaa cgccacgcat accctgcaca
    31441 acttctacac tctgcgcggc gcgaaaaccc gcgacaccag caagtggacc gagtatctga
    31501 acgaaacgct ggatatgtgg ggtaacgacg cggaagtcct gtttatgccg cacacctggc
    31561 cggtctgggg caataagcat atcaatgatt atattggtaa atatcgcgat actatcaagt
    31621 acattcacga ccagaccctg cacctggcga accagggcta caccatgaat gaaatcggcg
    31681 acatgattaa actgccgcct gcacttgcca ataactgggc cagccgtggc tattacggtt
    31741 ctgtcagcca caacgcccgc gcggtgtata acttctatct tggctattac gacggtaacc
    31801 cggctaacct gcatccgtat ggtcaggtgg agatgggtaa acgttacgtg caggcgctgg
    31861 gcggttctgc ccgtgtcatc aacctggcgc aagaagcgaa caagcaaggt gattaccgct
    31921 ggtcggcaga actgctgaaa caggtgattg ccgccaaccc gggtgaccag gtcgcgaaga
    31981 atctgcaagc gaataacttt gaacagctgg gctatcaggc cgagtccgcc acctggcgcg
    32041 gtttctacct gaccggcgcg aaagagctgc gcgaaggggt gcataagttc agccacggca
    32101 ccatcggttc cccggacacc attcgcggga tgtcggtcga aatgctgttc gactttatgt
    32161 ccgttcgcct cgatagcgcg aaagccgcgg gtaaaaatat cagcctgaac ttcaatatga
    32221 gcaatggcga taacctcaac ctgacgctga acgatagcgt gcttaactac cgtaaaacac
    32281 tgcaatccca agctgacgcc tctttctaca tcagccgtga agatctgcac gccgtgctga
    32341 ccgggcaagc caaaatggcg gatctggtaa aagcgaagaa agccaaaatt attggcaatg
    32401 gcgcgaaact ggaagaaatt atcgcctgtc tggataattt cgatttgtgg gtgaatatcg
    32461 taaccccaaa ttaatgtcac tcgcttttgg gggcgctgaa gcccccaaaa gcatttattt
    32521 taaaaatata tacagccaat taataaataa aatatataag ttatgaatgc ggtgactgaa
    32581 taaagctatt tttattcatc gagaaaatag ccatactgac gccttcaatg gatattgata
    32641 ttcactacag atattgttcg cagcagacag gatgtattta atatgccaac agttctctct
    32701 cgcatggcga tgcaactcaa aaaaacagcc tggataattc ccgtcttcat ggtttcggga
    32761 tgctcattat ctccggcaat cccggtgatc ggtgcttatt atcccagctg gtttttctgc
    32821 gccattgcca gccttatttt gatgctcatc acgaggcgag ttattcagcg ggcaaatatc
    32881 aatctggcat ttgtcggaat tatttatacc gccctttttg ctctctacgc catgctgttc
    32941 tggctggcat ttttctaatc tatttgagac gcgctcatgg aaagtacgcc gaaaaaagct
    33001 cctcgcagta aattccctgc tctgttagtg gttgcattgg cgctggttgc ccttgttttc
    33061 gttatctggc gcgtagacag tgcgccatca actaatgacg cttacgcgtc agcagatacc
    33121 attgatgtgg tgccggaagt cagcggccgc attgtagaac tggcggtcac cgacaaccag
    33181 gcagtcaaac agggcgattt gctgttccgc attgacccgc gcccgtacga agccaatctg
    33241 gcgaaagctg aagcctccct cgcggcgctg gataagcaaa ttatgctcac ccagcgtagc
    33301 gttgatgcgc aacagtttgg tgccgactcg attaatgcca cggtagaaaa agcccgtgcc
    33361 gccgcgaaac aggccacaga tacattacgc cgcaccgagc cattactgaa agagggtttt
    33421 gtctcagcgg aagacgttga ccgtgcaaga actgcgcagc gcgccgcaga agccgatctt
    33481 aatgccgtat tgttacaggc gcagtcagcc gccagcgccg tcagcggcgt cgatgcgctg
    33541 gtggcgcaac gtgcggcggt cgaagcggat attgccctga ccaaactgca tctggaaatg
    33601 gcgaccgttc gcgcgccgtt tgatggccgg gtcatttccc tcaaaacctc cgtcgggcaa
    33661 tttgcttctg ccatgcgccc tatttttacc ctaatcgaca ctcgtcactg gtacgtgatc
    33721 gccaacttcc gcgaaaccga tctgaaaaat attcgctcag gtacacccgc aacgattcgc
    33781 ctgatgagtg acagcggcaa aaccttcgag ggtaaagtgg attcgattgg ctacggcgtg
    33841 ctaccggatg acggcggcct ggtgctgggc ggcctgccga aagtttctcg ctctattaac
    33901 tgggtccgcg ttgcccagcg ttttccggtc aaaatcatgg tcgataaacc tgacccggaa
    33961 atgttccgca tcggcgcttc ggcagtcgct aatcttgagc cgcaataatg agcgcgctca
    34021 actccctgcc attaccgatg gtcaggctgc tggcgttctt tcatgaagag ttaagcgagc
    34081 ggcgacccgg ccgcgtgccg cagatcgtgc aactctgggt aggctgcctg ctggtgattc
    34141 tgatctcgat gacttttgag atcccttttg tggcgttatc gctggcagtg ctgttttacg
    34201 gtattcagtc gaacgcgttt tacaccaaat ttgtggcgat cttgtttgtg gttgccacgg
    34261 tgctggagat cggcagcctg tttttgatct acaaatggtc atacggcgaa ccgttgatcc
    34321 gattgatcat cgccgggccg atcctgatgg gccgcatgtt tttgatgcgc acccatcggc
    34381 tgggactggt ctttttcgct gtcgccattg tcgccattta cgggcaaacc ttccccgcca
    34441 tgctcgacta tccggaagtg gtcgtgcgct taacgctgtg gtgtatcgtt gttggcctct
    34501 atccaacctt gctgatgacg ttaatcggcg tgctgtggtt tcccagtcgt gccatttcgc
    34561 aaatgcatca ggcgcttaat gatcggcttg atgatgccat tagtcacctg acagacagcc
    34621 tcgcaccgct acccgaaacg cggattgaaa gagaggcgct ggcgctgcaa aaactgaatg
    34681 tcttttgtct cgcggacgat gccaactggc gaactcaaag cgcatggtgg caaagctgcg
    34741 tggcaacggt aacctacatt tactcgacgc tgaatcgcta cgatcccacc tcttttgctg
    34801 attctcaggc aattattgaa tttcggcaaa aattagcctc agaaatcaac aagctgcagc
    34861 atgccgttgc tgaaggtcag tgctggcaaa gcgactggcg gatcagtgaa agtgaagcgg
    34921 tggcggcacg ggaatgtaac ctggagaata tctgccagac gttgttacaa ctgggccaga
    34981 tgaacccgaa tacgccgcca acgcccgccg ccaaaccgcc atcaatggtc gccgatgctt
    35041 ttaccaatcc agactatata cgctacgcgg taaaaacatt gctcgcctgt ttgatctgtt
    35101 acacctttta cagcggcgtg gactgggaag gcattcacac ctgtatgctg acctgcgtga
    35161 tcgtctctaa tccaaatgtc ggttcgtcgt accagaagat ggtgctgcgt tttggcgggg
    35221 ccttttgcgg cgcgattctg gcgctgttat tcacgctact ggtcatgccc tggctggaca
    35281 atattgtcga attgctgttt gtgctggcac cgattttcct gttgggcgca tggattgcca
    35341 ccagctctga acgctcttct tatatcggca cacagatggt ggtcaccttc gcgctcgcca
    35401 cgctcgaaaa cgtttttggc ccagtgtacg acctggtgga aattcgcgat cgcgccctgg
    35461 gtatcatcat tggtaccgtg gtgtccgcgg tgatttacac ctttgtctgg cctgaaagtg
    35521 aagcgcgcac gctgccgcaa aaactggctg gcgcgctggg tatgttaagt aaagtaatgc
    35581 ggatcccacg ccagcaggaa gtcacggctc tgcgcactta tctgcaaatt cgtatcggtc
    35641 tgcatgcggc gtttaatgcc tgtgaagaga tgtgccaacg cgtggcgctg gagcgtcaac
    35701 tggacagcga agaacgcgcc ttactgattg aacgttcgca aacggttatt cgtcagggcc
    35761 gcgatatcct tcacgcctgg gatgccacct ggaattcggc gcaggcgctg gataacgcac
    35821 tacagccgga cagagcaggt cagtttgccg acgccctgga gaaatacgct gccggtctgg
    35881 caaccgcact cagccgttct cctcaaataa cgcttgaaga gacacccgcc tcgcaggcca
    35941 tcctgcccac cttattaaaa caggagcaac acgtctgcca gcttttcgcc cgcttgccag
    36001 actggacagc cccggcatta acgcccgcca cggaacaggc acaaggagcc acgcaatgat
    36061 caatcgtcaa ctttcacgtc tgctgttgtg cagcattctc ggcagcacga cgctgatttc
    36121 cggctgtgcc ttggtacgca aggattctgc gcctcatcaa cagctcaaac cggaacaaat
    36181 caaactggcc gatgatattc atcttgccag ctccggctgg ccgcaggcgc agtggtggaa
    36241 acaactcaat gacccgcagc tggatgcgct gatccaacgg acgctaagtg gttcacacac
    36301 cctcgccgaa gcgaaactgc gggaagaaaa agcgcagtcc caggccgatt tgttagatgc
    36361 cggttcacaa ttacaggtcg cagcgttagg gatgctcaac cgccaacgcg tctcggcgaa
    36421 cggcttttta agcccttatg cgatggatgc gcccgcactg ggtatggacg ggccgtacta
    36481 tacggaagcc acagtaggtt tgtttgccgg actggatctt gatttgtggg gtgtgcatcg
    36541 ctcagcggtt gccgccgcca ttggcgcgca taatgccgcg ctggcagaaa ccgcagcagt
    36601 agagctatcg ctgaccacgg gcgtagcgca gctttattac agtatgcagg ccagctatca
    36661 gatgctcgat ctgttagaac aaactcgcga tgtgattgat tacgcggtga aagcgcacca
    36721 gagtaaagtg gcgcacggtc tggaagcaca agtgcctttc cacggcgcgc gggcacagat
    36781 tctggcggtc gataaacaaa ttgctgccgt caaagggcaa atcaccgaaa cgcgagaatc
    36841 tctgcgtgca ttgattggcg cgggggccag cgatatgccg gagatcaaac cggtggcatt
    36901 accacgagtc cagaccggca ttccggcgac actctcttat gagttgctcg ccagacgccc
    36961 ggatctgcaa gccatgcgct ggtatgttca ggcgtcatta gatcaggtgg attccgcgcg
    37021 ggcgctgttc tacccgagct ttgatatcaa agcgtttttc ggccttgatg ccatccacct
    37081 ggatacctta ttcaaaaaaa ccagtcgcca gttcaacttc atcccgggtc tgaaattgcc
    37141 gctgtttgac ggtggacggt tgaatgccaa tctcgaaggc acgcgcgccg ccagcaacat
    37201 gatgattgaa cgttacaacc agtcagtact gaacgcggtg cgtgacgttg ccgtcaacgg
    37261 cacgcgtctg caaacgctca acgacgagcg agaaatgcag gctgaacgcg tggaagcaac
    37321 gcgcttcacc cagcgcgctg ccgaggccgc ctatcagcgc ggcttaacca gccgcttaca
    37381 ggccactgaa gcccggttgc cagtacttgc cgaagagatg tcattactga tgctggacag
    37441 ccgccgggtg atccaaagca ttcagttgat gaaatcgctg ggcggcgggt atcaggcggc
    37501 tcccgtcgtc gagaaaaaat aaaatgtctg ccgcgtgatg gctgtcacgc ggtatttcgt
    37561 ttcgtcacgt caaaactgac gacagcctgt ttttcgtcag agttttgaat aaatagtgcc
    37621 cgtaatatca gggaatgacc ccaaataaaa tgtggcataa aagatgcata ctgtagtcga
    37681 gagcgcgtat gcgtgatttg attaactgga gcgagaccga tgaaaaaagt cgtcacggtt
    37741 tgcccctatt gcgcatcagg ttgcaaaatc aacctggtcg tcgataacgg caaaatcgtc
    37801 cgggcggagg cagcgcaggg gaaaaccaac cagggtaccc tgtgtctgaa gggttattat
    37861 ggctgggatt tcattaacga tacccagatc ctgaccccgc gcctgaaaac ccccatgatc
    37921 cgtcgccagc gtggcggcaa actcgaacct gtttcctggg atgaggcact gaattacgtt
    37981 gccgagcgcc tgagcgccat caaagagaag tacggtccgg atgccatcca gacgaccggc
    38041 tcctcgcgtg gtacgggtaa cgaaaccaac tatgtaatgc aaaaatttgc gcgcgccgtt
    38101 attggtacca ataacgttga ctgctgcgct cgtgtctgac acggcccatc ggttgcaggt
    38161 ctgcaccaat cggtcggtaa tggcgcaatg agcaatgcta ttaacgaaat tgataatacc
    38221 gatttagtgt tcgttttcgg gtacaacccg gctgattccc acccaatcgt ggcgaatcac
    38281 gtaattaacg ctaaacgtaa cggggcgaaa attatcgtct gcgatccgcg caaaattgaa
    38341 accgcgcgca ttgctgacat gcacattgca ctgaaaaacg gctcgaacat cgcgctgttg
    38401 aatgcgatgg gccatgtcat tattgaagaa aatctgtacg acaaagcgtt cgtcgcttcc
    38461 cgtacagaag gctttgaaga gtatcgtaaa atcgttgaag gctacacgcc ggagtcggtt
    38521 gaagatatca ccggcgtcag cgccagtgag attcgtcagg cggcacggat gtatgcccag
    38581 gcgaaaagcg ccgccatcct gtggggcatg ggtgtaaccc agttctacca gggcgtggaa
    38641 accgtgcgtt ctctgaccag cctcgcgatg ctgactggta acctcggtaa gccgcatgcg
    38701 ggtgtaaacc cggttcgtgg tcagaacaac gtgcagggtg cctgcgatat gggcgcgctg
    38761 ccggatacgt atccgggata ccagtacgtg aaagatccgg ctaaccgcga gaaattcgcc
    38821 aaagcctggg gcgtggaaag cctgcctgct cataccggtt atcgcatcag cgagctgccg
    38881 caccgcgcag cgcatggcga agtgcgtgcc gcgtacatta tgggcgaaga tccgctgcaa
    38941 actgacgcgg agctgtcggc agtacgtaaa gcctttgaag atctggaact ggttatcgtt
    39001 caggacatct ttatgaccaa aaccgcgtcg gcggcggatg tcattttgcc gtcaacgtcg
    39061 tggggcgagc atgaaggcgt gtttactgcg gctgaccgtg gcttccagcg tttcttcaaa
    39121 gcagttgaac cgaaatggga tctgaaaacg gactggcaaa tcatcagtga aatcgccacc
    39181 cgtatgggtt atccgatgca ctacaacaac acccaggaga tctgggatga gttgcgtcat
    39241 ctgtgcccgg atttctacgg tgcgacttac gagaaaatgg gcgaactggg cttcattcag
    39301 tggccttgcc gcgatacttc agatgccgat caggggactt cttatctgtt taaagagaag
    39361 tttgataccc cgaacggtct ggcgcagttc ttcacctgcg actgggtagc gccaatcgac
    39421 aaactcaccg acgagtaccc gatggtactg tcaacggtgc gtgaagttgg tcactactct
    39481 tgccgttcga tgaccggtaa ctgtgcggca ctggcggcgc tggctgatga acctggctac
    39541 gcacaaatca ataccgaaga cgccaaacgt ctgggtattg aagatgaggc attggtttgg
    39601 gtgcactcgc gtaaaggcaa aattatcacc cgtgcgcagg tcagcgatcg tccgaacaaa
    39661 ggggcgattt acatgaccta ccagtggtgg attggtgcct gtaacgagct ggttaccgaa
    39721 aacttaagcc cgattacgaa aacgccggaa tacaaatact gcgccgtgcg cgtcgagccg
    39781 atcgccgatc agcgcgccgc cgagcagtac gtgattgacg agtacaacaa gttgaaaact
    39841 cgcctgcgcg aagcggcact ggcgtaatac agtcctttct acagcctcct ttcggaggct
    39901 gtttttttat ccattcgaac tctttatact ggttactccc tacccaatcg tattatcaaa
    39961 atgaaaaaaa ttatcgcatt gatgttgttt ttgacattct ttgcccacgc caacgactcc
    40021 gagcctggca gccagtattt aaaggcagca gaggccgggg accgacgcgc acaatatttt
    40081 cttgccgaca gctggtttag ctccggcgat ttgagcaaag ccgaatattg ggcacagaaa
    40141 gccgccgaca gcggtgatgc tgatgcctgc gcgctgctgg cgcagatcaa aatcaccaat
    40201 ccggtcagtc tggactatcc acaagcaaaa gttcttgcag agaaagcggc gcaagcgggc
    40261 agtaaagaag gtgaagtaac gctggcgcat attctggtaa atactcaagc gggtaaaccg
    40321 gattatccaa aggctatttc gctgttagaa aacgcctcgg aagatctgga gaacgactct
    40381 gccgtcgatg cccaaatgct gcttggtttg atttacgcca acggcgtggg cattaaggcc
    40441 gacgatgaca aggcaacctg gtatttcaaa cgcagctctg caatttcccg aaccggttat
    40501 tccgagtact gggcgggaat gatgttctta aatggtgaag aaggttttat cgagaagaac
    40561 aagcaaaagg cgctgcactg gttgaacctg agctgtatgg aagggtttga taccgggtgt
    40621 gaagagtttg aaaaattaac caacggttaa ggtaggcctg ataagacgcg gcaagcgtcg
    40681 catcaggcat tcacacattg ccgggtggat atccccggca atcttcaatt attgatccgc
    40741 agttttatca aatttgccca gcacttcacg ctcataagcc agcgcctttt tgcggtcaaa
    40801 tttgtgttcc cacttggcaa tcaccagcac cgccagcgca ttacccacca cgttcagcgc
    40861 agtacgcgcc atgtcgagga tacggtcaac accagcaata aacgccagac cttccagcgg
    40921 aatacctacg ctacccagcg ttgccagcag caccacaaac gacacgcccg gcacgccagc
    40981 aatccctttc gaggtcacca tcagcgtcag caccagaatg atttcctgcc agatggacag
    41041 gtcaatgcca tacagctgag cgatgaaaat agcggcaata ctttgataca gcgtcgaacc
    41101 atcaaggtta aaagagtaac cggtcggcac cacgaagctg gtgatcgacg ccggtgctcc
    41161 gtaggcttcc atcttctcaa taattcgcgg cagtacgctt tcagagctgg cagtggagta
    41221 cgccagaatc agctcatctt tcagaatacg aatcaggatc cagacgctta acccgcacag
    41281 gcgcgccaca attcccagca ctaccagcgc gaagaacaga atggcgaaat gcaccagcag
    41341 caccagtttc gccagtggcc acagagaaga gaaaccaaag ttagccaccg tcaccgcaat
    41401 cagcgcaaac acacccaccg gtgcataacg catcaccatg tgagtcactt taaacatggt
    41461 ttcagagatg gagcggaaca cggtcaccag cggttcacga tgcgtcgcgg gcagggaaga
    41521 aagccccaga ccaaacagca ccgagaaaaa gatgatcggc agcatttcgc ctttcgccat
    41581 cgacgccaca atgttcgtcg gcaccagcga caaaatcgtg cccataatgc cgtgggaact
    41641 gctttgtacc gcttccgtag tgctctgata tttcgagata tcgacggtcg ccagctgcga
    41701 catatccacc ccggcaccgg gctggaagac gttcgccaga gtgatcccca aaatgatggc
    41761 gacggtggtg atcacctcga agtaaataat ggttttcgcg ccaatacgcc cgagctgttt
    41821 ggcatcacca acacccgcga tacccaccac cagcgtggag atcacaatcg gcacaacaat
    41881 cattttgatc agatggatga agatatcacc cgccggagag agcaaattga cgaccagcca
    41941 gtcgcggctg tcgctatggt agtgcaggta gcttcccagg agaatgccca gcaccatagc
    42001 aaacagaatc tgccaggcca ggctgaattt tatatttttc atcagaataa cttcctcaat
    42061 ggagcgccgt tccccgttca gaaacgcgag aacgtgacat actgccaggg catgaattgt
    42121 gttgcgttgg tttgtagatt tttttaacgc ttatgatcag tatcatttgc gcggtatgtt
    42181 gcgcaaccct ttaacaacag agcttttcgc tgcattaatg cataataacg cgagtttatg
    42241 gcaggattaa tgaataacca tttgttatgg aaagattttt atttgtttaa tctgcaataa
    42301 tactgattag tgattatttt tggcatatgt tcattcgatt attttttatg cagctcaatg
    42361 acataatctt catcaccaga tgattaatcc gatctccgct gcaacagttt tgccatatta
    42421 atcgactcga ccagctgtgt ccggttgatc cggggtgagt tgagatccat cactttttgc
    42481 catagttcga tggcttgcgc gtaattcgcc tgcataaacg catcggaagc cagcagcatc
    42541 agagcggtga tttcgttaga gtccagcgcg agggctttgt cgatcattgc gcaagtctgg
    42601 gcggtcatat gctggctggc ctggtaataa agcaccgtcg ccagcgccgc atacagttca
    42661 gcgttctcac cacgtagttg caacgcctga cgatacgcca gcagcgaatt gctgtaatcg
    42721 ttttgccaca gatagtactc gcccagtaac gcccattgtt cgctgttttg tggattagcg
    42781 cggattttat cctgcaatgc ctgaagctgc gcttccgggg tttgctggct ggcaaactga
    42841 tgtagcggat cgcgctgacg ctgatactcc gcacgtaccg cctgccattt tgggcttaac
    42901 agataactgc cgacacagag gaataccatc aatatcgtga gtgttgtcag cattttcacc
    42961 ggtatttgag gctgtttcat cgcttagccc tcactcgcca gaggatcagt gccatcagca
    43021 ataacaacac cactggcagc gcccacaaca ccagcgtctg acccgttaac ggtgggttat
    43081 agcgaacaaa atctccgtag cgttcggtca tccagccaat gatttcgact tcgttcttcc
    43141 cctccgacac catgctataa acctgatggc gcatactgac cgccaccggc gcattggatt
    43201 cgagtaagtt ttgattctgg cactgcggac aacgtaactg gctggcaata tttaacgcct
    43261 gttgctgttg ttgcggattg gcgaattgcc aggtgtctac gacctgagcg cgcgcaaaac
    43321 aggtaaataa cagcagtagc gtgagaagcc ctttattcat cgcgcttctt cccccgccat
    43381 ccgcttaaca atgcgcccgc aatcatcaac aatcctccac cccagatcca gcgcacaccg
    43441 ctttgtacat acaaacgaaa agcgtaacga tccgctccgg ttttttcacc catgaccgca
    43501 taccagtcat ggatgccgtt ccagcgaatt gacggttcca tcatttgctg acggcgtgct
    43561 tcataaaaac gccgctccgg cgttaattca ccaatgcgtt gctgatggtc aaacagcgcc
    43621 actatcgctt tttcgctggt gtaattgcct ttggcttgca gatcgaggcg ctcaaaacgg
    43681 aaggtgtatc ctgccagcgt cacctgctga cccggctgta aattcaggct gatctcctga
    43741 cggctgacgc tggaaaccac gatccctgcg gcaaataaca gcacgcccgc atgagcgacc
    43801 agcgccggaa gctgcgcgcg tttgccagag acaaacgtcg ccagcacaat caccaccagc
    43861 atcaacagac caaacggtaa cgtcgcgcgg ttaaaatacg gcgcgccaac ggagaggcgt
    43921 ccccagccca gcagaccgta aatcatcgga taaagcgttc ccaccagcac gatcagtagc
    43981 actgcgcaaa acagcaacag cgtagcgagg attaacattt cccgcgataa ccccgaaaaa
    44041 cgcaccgccg ggccaccgtc ccgcgctcgc cagccataca gagccaaaga cgcaaggcta
    44101 atcagtgcaa acaggctgaa caacggcacg gcgcggacgt tatccagtgc gaacgcatga
    44161 accgaaacca gaatgccaga acggacaatt aaggtgccca gcagcgacag catcagagta
    44221 actatcgcca gcaacagcga ccagtggcaa aaaatccccc gctggcgtgt cagggataaa
    44281 ctgtgcagca gcgcagtggc agaaagccag ggtaataaag aggcattttc caccggatcc
    44341 cagaaccacc agccgcccca gccgagttcg cagtaggccc accaggaacc gaggatgatc
    44401 cccgccgtta atgcactcca gccaggtaac gcccagcgcc agcaaattcg ggcgctggcc
    44461 gcatcaaact cgccgcgcag tagactcgcc agcgccacgc tcgccgctac catcaaaccg
    44521 ccatagccaa ggtaaagcag cggtggatga aagataagac cgggatgttg cagcatcgga
    44581 ttgagatcgc ggccttcgat tgctggtgga aatatccgca cgaagggatc ggaccacagc
    44641 accacaaaca gtagcagtgc ggcgagcatg agagataaaa cggctaacgt cagcggaaat
    44701 agcggatcgg tttgctgccg ataatgccag gcaaacagcg cgctccaggc ggaaagcagc
    44761 agcacccaaa gcagcagcga accttcatgg ccgccccaca ccgctgccag cttgaattcc
    44821 cacgacagca ggctatagct atgttgcgcg acataaatga ccgagaaatc gctgatgaga
    44881 aaacaatacg tcagtacgcc aaaggcgagc agcaggagcg cgaactgcgc cagaatgccg
    44941 atgcaagtga gtcgcatcat ggcaggccag cgcaaccgca ctcccgcaaa ggccgtcaac
    45001 ggggtcaaca cgttgacccc gagacttaac aacaacgcca gaaaaccgac ttcaggaagg
    45061 aatacgtcca gaaaccacct cttttctggc ttacgccacc gtcaactgtc cggcgtagag
    45121 aatgaaaaag cgcagcatca acacgccgac cagactggcc ccacacgccg ccagcacggc
    45181 aggaatgccg gaactgcgat tgacccacgg tttgagcaac attggcacaa tcagccccag
    45241 cccggcgaca ccaagccaga accaccacgt ccagaaaccg ccacctaatg ccgccaccag
    45301 cgcacgaact ttaccgtcat cgcccagcgc cagaccgaca aaaaacgcca ccagcaggaa
    45361 gatttcaccc cataccacgg ggatttccat ccggtgaaca aagtgcgctt ccgtggaatg
    45421 cgggttactg cgttggcgta tcgccatggc gatcagcgcc accgccgcac cggacgagat
    45481 gccggagaag aggaacagca ccggcaggat cgggttattg aggaacggat acgatttcag
    45541 cgccgacagc agaaagccgg tataagcccc cagcagcacc gccaacacca gcatcagcgt
    45601 ttccagtccg cgatgaacgg gcgtcagcag gctcagaacc ttttgcacga tccccagctt
    45661 cggcaaccag cgttgttgca gggcaagcaa atcatgttca aagattttcg ccagccacag
    45721 caccagcacc accatgtaga gctgaaacag catcaccccc atcgacatca ccgaggtaaa
    45781 actgtagtgg aacatcagct tccagaaggt ccacggtctt gtcaggtgga agacgaggat
    45841 caacagaccg aggatcaccg cgcccggccc gacaatcagc gtggtgcgca gcaacgtact
    45901 gtctgcaccg cccgcctgcg ggtagaagcg acgtaacagc acggccagcg tcaccagacc
    45961 ggcagaaata ccaatcaaaa acaggtagat ggcaatcggc cagtcccaca ccagcgattc
    46021 aaaatgaaat gcggaagtct gcgtcattgg ctcacctccc cgtatttaaa cggtacgcgg
    46081 tacagcttcg gtttggttcc cagcgccagc ttgtagcggt aagtaggctt ctggcgcagc
    46141 agttgcgaaa tctcactgtt aggatcgtcc agattgccaa acgtcagcgc cttcgtcggg
    46201 caagcctcaa cacacgcggg cagcttaccg gcctgcaaat tggttttacg gcagaaatcg
    46261 catttgtccg ccgtcttcgt gaccggatgg ataaagcgca cgcggtacgg acaggcggcg
    46321 atgcagtact gacaaccgac gcagagatca ggattaacat cgacaatccc gctagcggca
    46381 tcacgaaaag acgcaccggt cgggcagacg tcaacgcacg gcgcatgatc gcagtgctgg
    46441 caagacttac ggaagaagcg atatttcaca tcaggaaatt cgccctgcgg ctcgctacga
    46501 ataatcgtca ggcgcgagac gccttccggc accttgttca cttcccgaca agcatccata
    46561 caggcggtac agccgatgca taacgactcg tcatgcacca tgccgtaacg caccccattg
    46621 atattcaacg ttttcgccac cacgcgccct gcggtgccgc tgacggctgc cagcacgccg
    46681 acgccggtga gaaactggcg acgagaccag gtcatggctg ctccttaagc aacggaacag
    46741 acgccgggtt aaagttcgga ttggtgcgct gatcgctgtg gcaatcgacg caaatcttaa
    46801 tccgtccttt gtcacttaac gtctgcatcg tatcttgctg tggatgcagg gaatggcagc
    46861 tggcgcacgc cactttggtt acgtggacat cgtgcggcca gaacgctttt tgcaactgtt
    46921 ccggcagatg acaggacata cagacgctgt tctgctctcc aaccttgtac atcggctcgt
    46981 taaagcgcat cacatctttc accccttcgc ggtgttgcgg tgatggctgg ccgtgacagt
    47041 tggtgcaggt gaccggcagt ttgttattcg ggttgataac ggaagcatgt ttgccatgca
    47101 taccttcggt atctggttta tgacagtcca gacaggcggc atccgggtta cgctgctggg
    47161 taacttcgta gcgatcatca gatgcctgcg ccgcaggggt agcggtaatt ccgttcaggc
    47221 tccacaacag gcccgacgcc agcaccccgg cagttaataa cgaacgtaat acgctcatat
    47281 tcactccatt gtgttttccg ccccttgcga ggcggaacgg ggttattggc ttaacagacc
    47341 gtttttacgt gcctgttctt cccactgcgg gatcaccgtt ttgatgaagt cctgcttctc
    47401 ggccttgatt tgttccatgt tcaggccaat ggcctgctgg gctttctctt tggttgagat
    47461 atccgggatc tggatttcat gggtgatgcc tttggtcgcc agcaggcgcg ccagtttggt
    47521 gcgtgcatcc gccgctttat ccatcgccgt accgagcatc cgtaaacctt cttccggtgc
    47581 gtgcatatga atgccgtggg aagcgatcgc cagatcccag cgccactggg catgacggat
    47641 atcgtcctga attggcttca tttcagcttc cgtcgcgcct gcatccagcg ctgctttcgc
    47701 ttcgaagtga gcgtgaacca gttgatcttc aaccttgatt ttcaggtcgt taatcgactg
    47761 cttacgttcc gcgaccactt tttgcaaggc agctttgtcc tgggtatggc agttcgcaca
    47821 agtctgggcg aagttatcaa acggattacc aattttatgg tcggtataga gtttgccttc
    47881 ggcgttctgc acttttggca tatggcagtc gatacaggtc acgttgtttt taccgtgaat
    47941 gcccgctgtc caggtttcat attccgggtg ctgcgctttc agcattggcg ttttcgacag
    48001 ggagttagtc cagtcagaga aggcaatttt gtcgtaatac tgctccatat tttcgacttt
    48061 catgccgtca tcccacggga atttaaccgc tttgtttttg ccgtcgaagt aatactccac
    48121 atggcactga ccgcaaacca tcgattgctg gtcgaaacgt ccggctttct caaaaggttt
    48181 accaatggct tccatcgcgc gagccgcata cggacgggaa agggttaact ccggtttgcc
    48241 tttggcgaac tctggtgagg cggtgttatg gcagtcggca caacctaagt tgttgacgat
    48301 ttccgggccg ccgcgcgccc acttaccgtg gaagtagcca tcttcgccgt ctttctggat
    48361 cagacgcgcc acatccgggc ttttacaact ccagcacgcc atcggtagcg gaccatcttc
    48421 agcgtttttc ggcgcaccgg tacgcagggt ttcacgcaca tcggtcacag caaaggcatg
    48481 tccacgcggc ttgttgtaat cgcgcgagaa gggatacccc gcccacagga tcaccagccg
    48541 tggatcttcc gccagggcgt caacacgctc tgactgttcc gaggtggctt tccaggagag
    48601 atattgatcg ggatgctgcg gggcaaaggt ttcattcttc gcttccacag ttacaggttt
    48661 tgcgggagct gccgtttgtt cagcgtgaac agaagtgaaa aagaaaaaag gaatcaataa
    48721 gctgaagata cggcgtgcgt ttatttttat ccttgtcata ggggcttcat ccgaattgcg
    48781 ccattgttac aatggcggtt tttattgttt ttcacgacag taaccgcacc tacactgtca
    48841 tgacattgct cgcccctatg tgtaacaaat aaccacactg tgaatgttgt ctttaatcaa
    48901 ttgtaagtgc atgtaaaata ccactttaga gttagttagt atcttcctct ttttcaacag
    48961 caatgcataa ctgcatgttc ctcaaagaat taatcaactt ttgttgctga ccttcaaaaa
    49021 ttaccctgcc gtttatttgc acaattctac ttttgcgtga tctgtcgccc aaatactaaa
    49081 caaaactgcc aataccccta catttaacgc ttatgccaca tattattaac atcctacaag
    49141 gagaacaaaa gcatgagcca aattcacaaa cacaccattc ctgccaacat cgcagaccgt
    49201 tgcctgataa accctcagca gtacgaggcg atgtatcaac aatctattaa cgtacctgat
    49261 accttctggg gcgaacaggg aaaaattctt gactggatca aaccttacca gaaggtgaaa
    49321 aacacctcct ttgcccccgg taatgtgtcc attaaatggt acgaggacgg cacgctgaat
    49381 ctggcggcaa actgccttga ccgccatctg caagaaaacg gcgatcgtac cgccatcatc
    49441 tgggaaggcg acgacgccag ccagagcaaa catatcagct ataaagagct gcaccgcgac
    49501 gtctgccgct tcgccaatac cctgctcgag ctgggcatta aaaaaggtga tgtggtggcg
    49561 atttatatgc cgatggtgcc ggaagccgcg gttgcgatgc tggcctgcgc ccgcattggc
    49621 gcggtgcatt cggtgatttt cggcggcttc tcgccggaag ccgttgccgg gcgcattatt
    49681 gattccaact cacgactggt gatcacttcc gacgaaggtg tgcgtgccgg gcgcagtatt
    49741 ccgctgaaga aaaacgttga tgacgcgctg aaaaacccga acgtcaccag cgtagagcat
    49801 gtggtggtac tgaagcgtac tggcgggaaa attgactggc aggaagggcg cgacctgtgg
    49861 tggcacgacc tggttgagca agcgagcgat cagcaccagg cggaaaagat gaacgccgaa
    49921 gatccgctgt ttattctcta cacctccggt tctaccggta agccaaaagg tgtgctgcat
    49981 actaccggcg gttatctggt gtacgcggcg ctgaccttta aatatgtctt tgattatcat
    50041 ccgggtgata tctactggtg caccgccgat gtgggctggg tgaccggaca cagttacttg
    50101 ctgtacggcc cgctgacctg cggtgcgacc acgctgatgt ttgaaggcgt acccaactgg
    50161 ccgacgcctg cccgtatggc gcaggtggtg gacaagcatc aggtcaatat tctctatacc
    50221 gcacccacgg cgatccgcgc gctgatggcg gaaggcgata aagcgatcga gggtaccgac
    50281 cgttcgtcgc tgcgcattct cggttccgtg ggcgagccaa ttaacccgga agcgtgggag
    50341 tggtactgga aaaaaatcgg caacgagaaa tgtccggtgg tcgatacctg gtggcagacc
    50401 gaaactggcg gtttcatgat cacgccgctg cctggcgcta ccgagctgaa agccggttcg
    50461 gcaacacgtc cgttcttcgg cgtgcaaccg gcgctggtcg ataacgaagg taacccgctg
    50521 gaaggcgcta ccgaaggcag cctggtgatc accgactcct ggccgggtca ggcgcgtacg
    50581 ctgtttggcg atcacgaacg ttttgaacag acctacttct ccaccttcaa aaatatgtat
    50641 ttcagcggcg acggcgcgcg tcgcgatgaa gatggctatt actggatcac cgggcgtgtg
    50701 gacgacgtgc tgaacgtctc cggtcaccgt ctggggacgg cagagattga gtcggcgctg
    50761 gtggcgcatc cgaagattgc cgaagccgcc gtagtaggta ttccgcacaa tattaaaggt
    50821 caggcgatct acgcctacgt cacgcttaat cacggggagg aaccgtcacc agaactgtac
    50881 gcagaagtcc gcaactgggt gcgtaaagag attggcccgc tggcgacgcc agacgtgctg
    50941 cactggaccg actccctgcc taaaacccgc tccggcaaaa ttatgcgccg tattctgcgc
    51001 aaaattgcgg cgggcgatac cagcaacctg ggcgatacct cgacgcttgc cgatcctggc
    51061 gtagtcgaga agctgcttga agagaagcag gctatcgcga tgccatcgta acccacaatt
    51121 gccggatgcg agtcggtaac ggtttgtagg cctgataaga cgcgacagcg tcgcatcagg
    51181 cattgattgc cggatgcggc gtaaacgcct tatccggcct acattcggca agggttaccc
    51241 gagcgttaac cttctcccat aagggagcgg gaattaaaac aatccctaca ttacctctgg
    51301 agaatctgtg atgaatggca ctatttatca gcggatagaa gacaatgcgc atttcaggga
    51361 gttagtcgaa aaacggcaac ggtttgccac catcctgtcg attattatgc tggcagttta
    51421 tatcggcttt attttactga tcgccttcgc gcccggctgg ctggcacgcc gctgaatccg
    51481 aacaccagcg tcacacgcgg tattccaatt ggtgttggag tgattgtgat ctcctttgtt
    51541 ctcaccggta tctacatctg gcgggcgaac ggcgaattcg accgtcttaa taatgaagtc
    51601 ctgcatgagg tacaagcatc atgaaaagag ttctgacggc gcttgccgcc acactccctt
    51661 tcgcagctaa cgccgcggat gctattagcg gggccgtaga gcgccagcca acgaactggc
    51721 aggcgattat tatgttcctg attttcgtcg tgtttacgct cggcattacc tactgggcat
    51781 caaaactcgt acgttctcgt agcgactact acaccgcagg cggcaatatc actggcttcc
    51841 agaacgggct ggcgattgcc ggggactata tgtccgccgc ctcattcttg gggatctccg
    51901 cgctggtgtt tacctccggc tatgacggct taatttactc gctgggcttc ctggtgggct
    51961 ggccgatcat tttgttcctg attgccgaac gtctgcgtaa cctg
//
LOCUS       DAKISA010000017        51227 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.017, whole genome shotgun sequence.
ACCESSION   DAKISA010000017 DAKISA010000000
VERSION     DAKISA010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 51227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51227
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.017"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            223..1125
                     /locus_tag="OVW45_001048"
     CDS             223..1125
                     /locus_tag="OVW45_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCV5525557.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPVFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            1272..2492
                     /locus_tag="OVW45_001049"
     CDS             1272..2492
                     /locus_tag="OVW45_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCV5525558.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPV
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQNLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            2465..3094
                     /locus_tag="OVW45_001050"
     CDS             2465..3094
                     /locus_tag="OVW45_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HCV5525559.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDKNAMEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(3095..4255)
                     /gene="ybdL"
                     /locus_tag="OVW45_001051"
     CDS             complement(3095..4255)
                     /gene="ybdL"
                     /locus_tag="OVW45_001051"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HCV5525560.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNSSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHSQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            4364..5452
                     /gene="hcxA"
                     /locus_tag="OVW45_001052"
     CDS             4364..5452
                     /gene="hcxA"
                     /locus_tag="OVW45_001052"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCV5525561.1"
                     /translation="MPHNPIRVVVGPANYFSHLGSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEMDINNHAEIDKVITHTLRP
                     VEYIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(5462..5659)
                     /locus_tag="OVW45_001053"
     CDS             complement(5462..5659)
                     /locus_tag="OVW45_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCV5525562.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(5841..7946)
                     /gene="cstA"
                     /locus_tag="OVW45_001054"
     CDS             complement(5841..7946)
                     /gene="cstA"
                     /locus_tag="OVW45_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCV5525563.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKSALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(8127..8540)
                     /gene="entH"
                     /locus_tag="OVW45_001055"
     CDS             complement(8127..8540)
                     /gene="entH"
                     /locus_tag="OVW45_001055"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCV5525564.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIIVFDEQGRRCCTCRLGTAVLG"
     gene            complement(8543..9289)
                     /gene="entA"
                     /locus_tag="OVW45_001056"
     CDS             complement(8543..9289)
                     /gene="entA"
                     /locus_tag="OVW45_001056"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCV5525565.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAVSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(9289..10146)
                     /gene="entB"
                     /locus_tag="OVW45_001057"
     CDS             complement(9289..10146)
                     /gene="entB"
                     /locus_tag="OVW45_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCV5525566.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEGLLPAPVPARKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(10160..11770)
                     /gene="entE"
                     /locus_tag="OVW45_001058"
     CDS             complement(10160..11770)
                     /gene="entE"
                     /locus_tag="OVW45_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCV5525567.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARISAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(11780..12955)
                     /gene="entC"
                     /locus_tag="OVW45_001059"
     CDS             complement(11780..12955)
                     /gene="entC"
                     /locus_tag="OVW45_001059"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HCV5525568.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSAHCFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            13330..14295
                     /gene="fepB"
                     /locus_tag="OVW45_001060"
     CDS             13330..14295
                     /gene="fepB"
                     /locus_tag="OVW45_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCV5525569.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYTLGTETFRLDYYSAMQVLDRLNSLLKKD"
     gene            complement(14376..15626)
                     /gene="entS"
                     /locus_tag="OVW45_001061"
     CDS             complement(14376..15626)
                     /gene="entS"
                     /locus_tag="OVW45_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCV5525570.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            15737..16741
                     /gene="fepD"
                     /locus_tag="OVW45_001062"
     CDS             15737..16741
                     /gene="fepD"
                     /locus_tag="OVW45_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCV5525571.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFIGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            16738..17730
                     /gene="fepG"
                     /locus_tag="OVW45_001063"
     CDS             16738..17730
                     /gene="fepG"
                     /locus_tag="OVW45_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCV5525572.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            17727..18542
                     /gene="fepC"
                     /locus_tag="OVW45_001064"
     CDS             17727..18542
                     /gene="fepC"
                     /locus_tag="OVW45_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCV5525573.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATRITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(18539..19672)
                     /gene="fepE"
                     /locus_tag="OVW45_001065"
     CDS             complement(18539..19672)
                     /gene="fepE"
                     /locus_tag="OVW45_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCV5525574.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(19888..23769)
                     /gene="entF"
                     /locus_tag="OVW45_001066"
     CDS             complement(19888..23769)
                     /gene="entF"
                     /locus_tag="OVW45_001066"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HCV5525575.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFEQPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNMGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(23766..23984)
                     /gene="ybdZ"
                     /locus_tag="OVW45_001067"
     CDS             complement(23766..23984)
                     /gene="ybdZ"
                     /locus_tag="OVW45_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCV5525576.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCLLSAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(23987..25189)
                     /gene="fes"
                     /locus_tag="OVW45_001068"
     CDS             complement(23987..25189)
                     /gene="fes"
                     /locus_tag="OVW45_001068"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCV5525577.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQV
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRDGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            25432..27672
                     /gene="fepA"
                     /locus_tag="OVW45_001069"
     CDS             25432..27672
                     /gene="fepA"
                     /locus_tag="OVW45_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCV5525578.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYQQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSEMVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            27847..28467
                     /gene="entD"
                     /locus_tag="OVW45_001070"
     CDS             27847..28467
                     /gene="entD"
                     /locus_tag="OVW45_001070"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCV5525579.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASER
                     QTEAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(28589..28741)
                     /gene="hokE"
                     /locus_tag="OVW45_001071"
     CDS             complement(28589..28741)
                     /gene="hokE"
                     /locus_tag="OVW45_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCV5525580.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(29092..29244)
                     /gene="hokE"
                     /locus_tag="OVW45_001072"
     CDS             complement(29092..29244)
                     /gene="hokE"
                     /locus_tag="OVW45_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCV5525581.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            29684..30802
                     /locus_tag="OVW45_001073"
     CDS             29684..30802
                     /locus_tag="OVW45_001073"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCV5525582.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWADD"
     gene            30868..31116
                     /locus_tag="OVW45_001074"
                     /pseudo
     CDS             30868..31116
                     /locus_tag="OVW45_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
     gene            31181..31549
                     /locus_tag="OVW45_001075"
     CDS             31181..31549
                     /locus_tag="OVW45_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCV5525583.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            31643..32296
                     /gene="nfsB"
                     /locus_tag="OVW45_001076"
     CDS             31643..32296
                     /gene="nfsB"
                     /locus_tag="OVW45_001076"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCV5525584.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            32404..33651
                     /gene="ybdG"
                     /locus_tag="OVW45_001077"
     CDS             32404..33651
                     /gene="ybdG"
                     /locus_tag="OVW45_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCV5525585.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(33719..35095)
                     /gene="pheP"
                     /locus_tag="OVW45_001078"
     CDS             complement(33719..35095)
                     /gene="pheP"
                     /locus_tag="OVW45_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCV5525586.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPDKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(35197..38340)
                     /gene="cusA"
                     /locus_tag="OVW45_001079"
     CDS             complement(35197..38340)
                     /gene="cusA"
                     /locus_tag="OVW45_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCV5525587.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIASVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPSVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(38352..39575)
                     /gene="cusB"
                     /locus_tag="OVW45_001080"
     CDS             complement(38352..39575)
                     /gene="cusB"
                     /locus_tag="OVW45_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HCV5525588.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALHSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATNAH"
     gene            complement(39591..39923)
                     /gene="cusF"
                     /locus_tag="OVW45_001081"
     CDS             complement(39591..39923)
                     /gene="cusF"
                     /locus_tag="OVW45_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HCV5525589.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(39947..41329)
                     /gene="cusC"
                     /locus_tag="OVW45_001082"
     CDS             complement(39947..41329)
                     /gene="cusC"
                     /locus_tag="OVW45_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCV5525590.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRTAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            41486..42169
                     /gene="cusR"
                     /locus_tag="OVW45_001083"
     CDS             41486..42169
                     /gene="cusR"
                     /locus_tag="OVW45_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCV5525591.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            42159..43607
                     /gene="cusS"
                     /locus_tag="OVW45_001084"
     CDS             42159..43607
                     /gene="cusS"
                     /locus_tag="OVW45_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCV5525592.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTEPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
     gene            44344..46245
                     /locus_tag="OVW45_001085"
     CDS             44344..46245
                     /locus_tag="OVW45_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCV5525593.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDSIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
     gene            46273..46725
                     /locus_tag="OVW45_001086"
                     /pseudo
     CDS             46273..46725
                     /locus_tag="OVW45_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            47669..>51227
                     /locus_tag="OVW45_001087"
     CDS             47669..>51227
                     /locus_tag="OVW45_001087"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCV5525594.1"
                     /translation="MAENDPGGNNDSQLSSAASGTTGKTEESGETRQTGKLLGKSGKL
                     LGAKLGALLGKTGKSLKSIANKVIRWVTDPVDPVTGAYCDERTDFTLGQTLPLSFTRF
                     HSSVLPLHGLTGVGWSDSWSEYAWVREQGNRVDIISQGATLRFAFDGDSDTAVNPYHA
                     QYILRRRDDYLELFDRDALSSRFFYDAFPGMRLRHPVTDDTSDDRLAHSPNDRMYMLG
                     GMSDTASNRITFERDSQYRITGVSHTDGIRLKLTYHASGYLKAIHRTDNDIQTLATYE
                     QDARGRLTEADARLDYHLFYEYDAADRIIRWSDNDQTWSRFTYDEQGRCVNVTGAEGY
                     YNATLDYGDGCTTVTDGKGIHRYYYDPDGNILREEAPDGSTTMYEWDEFHHLLARHSP
                     AGRVEKFEYNAAHGQLSRYTAADGAEWQYRYDERGLLSNITDPAGQTWTQQCDERGLP
                     VSLVSPQGEETRLAYTAQGLLSGIFRQDERRLGIEYDHHNRPETLTDVMGREHHTEYS
                     GHDLPVKMRGPGGQSVRLQWQQHHKLSGIERAGTGAEGFRYDRHGNLLAWTDGNGVVW
                     TMEYGPFDFPVARTDGEGHRWQYRYDKDTLQLTEVINPQGESYLYVLDNCGRVTEERD
                     WGGVVWRYRYDADGLCTARVNGLEETILYSRDAAGRLAEIISPEGKTQYAYDKSGRLT
                     GIFSPDGTSQRTGYDERGRVNVTTQGRRAIEYNYPDEHTVIRCILPPEDERDRHPGES
                     LLKTTYRYNAAGELTEVILPGDETLTFSRDEAGREVLRHSNRGFACEQGWNAAGQPVS
                     QRAGFFPAEATWDGLVPSLVREYRYDSAGNVSGVTSREDYGRETRREYRLDRNGQVTA
                     VTASGTGLGYGEGDESYGYDSCGYLKAQSAGRHRISEETDQYAGGHRLKQAGNTQYDY
                     DAAGRMVSRTRHRDGYRPETERFRWDSRDQLTGYCSAQGELWEYRHDASGRRTEKRCD
                     RKKIRFTYLWDGDSIAEIREYRDDKLYSVRHLVFNGFELISQQFSRVRQAHPSVAPQW
                     VTRTNHAVSDLTGRPLMLFNSEGKTVWRPGQTSLWGLALSLPADTGYPDPRGELDPEA
                     DPGLLYAGQWQDAESGLCYNRFRYYEPETGMYLVSDPLGLQGGEQTYRYVPNPCGWVD
                     PLGLAASSKISSLMDYIGDGRRVSGHTGFLDGVRLSRSQI"
BASE COUNT        12409 a        13996 c        13281 g        11541 t
ORIGIN      
        1 gggaaataaa taaaaggtaa ttcatggatg gttgaactat atccctgctt ttcaaccatc
       61 ctaaaaaagc atgaattgct cgctctaccc taagtggctg tgctatttta gaaaaactta
      121 atcctgagtt aagcaaaaaa acataatcaa taaaaataca tacgcaaaaa acattgatta
      181 agtgaatata ccatggaaga aaaatataaa ccggagtagt gtatggccaa tctctacgac
      241 ttgaaaaaat tcgacttaaa tcttcttgtc atatttgagt gtatttacca gcatttaagc
      301 attagcaaag ctgctgaatc actatatatc accccatcag ccgtgagcca gtcgttacaa
      361 cgcttacgtg cgcaatttaa tgaccctgta ttcattcgct caggaaaagg tatcgcgccg
      421 acgacaacag ggttgaatct gcatcaccat cttgagaaaa acctcaaagg ccttgaacaa
      481 acaattaata tagtcaataa gtccgaacta aagaaaaact ttattatcta cggccctcag
      541 ctcatttctt gctctaataa tagtatgctg atccgctgcc tacggcaaga tgcctctgtc
      601 gagattgagt gccatgatat cctcatgtca gcagagaatg ctgaagaact gttggttcac
      661 cgcaaagccg atctggtgat cacccaaatg ccagtgatca gccgttccgt catttgcatg
      721 ccattgcata caattcgcaa tactctaatt tgtagtaata agcatccgcg aattactgac
      781 aatagcactt atgaacaaat tatggcagaa gagttcaccc aattaatatc caaatcagca
      841 ggagttgatg atattcaaat ggaaatagac gagaagttta tgaatcgcaa aatttcattt
      901 cgtggttcct ctttgctgac aataattaat agcattgcag ttacagattt actgggaata
      961 gtcccctatg aactttataa ctcccaccgt gactttctta atttaaaaga gataaaacta
     1021 gaacatccgt taccttcaat aaagctttac atttcctata ataaatcttc tttaaataat
     1081 ttggtgttct cccgatttat tgaccgtcta aatgagagtt tttgatatat aactacagac
     1141 agctactata actttcatct atttattcac agcgcgaatg aaaaataaaa cactctgatt
     1201 tacagtgttt actttataat taacagtctt atctcactgt tcaccctgta acgttcagag
     1261 agcgtcttca gatgtctatt tataaaattc ctcttccgct caatattctg gaagctgcac
     1321 gagagcgtat tacctggaca ctcaacaccc taccccgcgt ttgtgtctca ttttctggcg
     1381 gcaaagactc tggtttaatg ctgcatctga cagccgaact tgcccgacaa atgggcaaaa
     1441 aaatctgcgt tttgtttatc gactgggagg cgcaattctc ttgcaccatt aactatgttc
     1501 agtccctgcg cgagttgtac accgatgtca tcgaagagtt ttactgggtt gcgctcccgc
     1561 ttacgacgca aaattccctt tcacaatacc aacccgaatg gcagtgctgg gaacctgatg
     1621 tcgaatgggt gcgtcagccc ccgcaagatg cgataaccga tcctgacttt ttctgctttt
     1681 accagccagg catgaccttc gaacaatttg tacgtgagtt tgccgaatgg ttttcacaaa
     1741 aacgtccggt ggcgatgatg atcggcatcc gtgcggatga gtcctacaac cgttttgtcg
     1801 ccatcgccag tttaaataaa caacgttttg ccgacgataa accatggact acggccgcac
     1861 caggcggtca tagctggtac atttacccca tttacgactg gaaagtggct gatatctgga
     1921 cctggtatgc aaatcatcaa aatctctgca acccactgta taacctaatg tatcaggcag
     1981 gcgttcctct gcgtcatatg cgaatttgcg aaccttttgg cccggagcaa cgacaaggat
     2041 tatggctcta tcacgttatc gaaccggatc gctgggctgc tatgtgcgca cgagtcagcg
     2101 gggtaaaaag tggcggcatt tacgccggac atgataatca tttctatggg catcggaaaa
     2161 tcctcaagcc agaacattta gactggcaag aatatgcatt attgctgctc aatagcatgc
     2221 cggaaaaaac agctgagcat taccgcaata aaatcgccat ttatttgcac tggtatcaga
     2281 aaaaaggcat cgaggttcca caaacccagc aaggggacat tggcgcgaaa gatatcccct
     2341 cctggcggcg gatatgcaaa gttttactca ataacgatta ctggtgtcgg gcattatcat
     2401 ttagtcctac gaaagcgaag aactatcagc gttataacga acggataaaa ggaaaacgtc
     2461 aggaatgggg gatactatgc aacaacgatt aacgcaggat ttaacgcaat ttctcgccag
     2521 cttgccggaa gatgatcgca tcaaagcgat taatgaaatc cgcatggcga tccatcaggt
     2581 tagccccttt cgtgaagagc cagtggattg cgttctgtgg gttaaaaaca gccaacttat
     2641 gcctaatgat tacaacccaa acaacgtggc accacccgaa aaaaaactgc tgcaaaaatc
     2701 cattgaaata gatggtttta cccaacccat tgtggtcaca catacggata aaaatgccat
     2761 ggaaatcgtg gatggttttc accgccatga aattggtaaa ggctcatcat cattaaaatt
     2821 acgcttaaaa ggttatttgc cggtgacctg tctggaaggt acgcgtaatc aacgcatcgc
     2881 cgcgactatt cgtcataatc gcgctcgtgg acgccatcaa atcaccgcca tgtcggaaat
     2941 cgtccgtgaa cttagccagt tgggatggga cgataataaa attggcaaag agctgggaat
     3001 ggacagcgac gaagtgctgc gcctgaagca aattaacggc ctgcaagagc tgtttgccga
     3061 ccgtcaatat tcccgcgcct ggacggttaa ataactaaag ctggcgcagg cgttcagctg
     3121 ctgccagcaa cgtcgattcc ttcttggcaa aacagagacg aatcagttta tgtgggaagg
     3181 gatcggcgca aaacaccgac agcggaatcg ccgctacgcc gtgctcctgc gtcagccact
     3241 ggcaaaactc aacatcatcc agggtagaaa ccgcgctgta atccaccagc aaaaagtatg
     3301 taccttcaca cggtaaaatc tccagccggc tttcatttaa ggcatttacc agaatatcgc
     3361 gcttctggcg ataaaagtcc ggtaacgcaa gataatgctc aggttctgca cgtagcatat
     3421 cagcaagcgc cagctgtgcc ggggtattca ccgaaaaggt cagatactga tgtaccttgc
     3481 gaatttcggc gctgatgggc gctggcgcaa cacaataacc cactttccag ccggtcatat
     3541 gataggtctt gccaaatgaa gaaaccgcca ctgcccgctc acgcagctgc gaatgcgcca
     3601 gcacactggc atggccctgt tgtgaaaagt tgatgtgctc gtagacttca tcgctaatga
     3661 caaaaatctc gtgcccggcg atcgcctgcc acaaagcggc gaaatcagcc tgctgccaga
     3721 cagttgcact ggagttatgc ggagtgttga ggatcaccag tctggtgcgc tcgcttaata
     3781 atgcggcaaa ttcctgccag tcaacgcgaa aatgcggtgg ttgcagtgcc atacgcttca
     3841 ctattccccc agaaagcgcg atggcggggg cgtaactgtc atagctggga tcaaaacaaa
     3901 tcacttcatc gccattgcgc accagtgcgg taatcgccgc gtataacgct tccgtcgccc
     3961 ctgccgttac ggtgatatcg ctatcggcat ctggttgata gccatacaaa cgttccgttt
     4021 tctgagcaat cgcctcgcgc aaggcctgca cgccggtcat gggcgcgtat tggtttgccc
     4081 cctgggcaac gtggtacgcc agccgctcct gtaaatagcg cggaccatca aaatcaggaa
     4141 agccttgcga caggttaatc gcctggtgtt gctgcgccag cgcgctcatc tgggtgaaaa
     4201 tagtggtgcc aagttgtgga agtttgcttt gtggaatcag agggttattt gtcattttta
     4261 ttgtacctga cgaagttgcg tgttgaaggc actataacac gatgctagta tttggcaatc
     4321 aagacgttta gatgtctaaa taaaacaata aggacaacac aacatgcctc acaatcctat
     4381 ccgcgtggtc gtcggcccgg ctaactactt ttcacatcta ggaagtttca atcacctgaa
     4441 cgattttttc actgatgaac aactttctcg cgcggtgtgg atctacggca aacgcgccat
     4501 tgctgcggcg caaaccaaac ttccgccagc gtttggactt ccaggggcaa agcatatttt
     4561 gtttcgtggt cattgcagcg aaagcgatgt acagcaactg gcggctgagt ccggtgacga
     4621 tcgcagcgtg gtgattggcg tcggcggcgg cgcactgctc gacaccgcga aagccctcgc
     4681 ccgccgtctc ggtctgccat ttgttgccgt tccgacgatc gccgctacct gcgccgcctg
     4741 gacaccgctc tccgtctggt ataacgatgc cggacaggcg ctgcattatg agattttcga
     4801 cgacgccaat tttatggtgc tggtggaacc agagattatc ctcaacgcgc cgcaacaata
     4861 tctgctggcg gggatcggtg acacgctggc gaaatggtat gaagcggtgg tgctggctcc
     4921 gcaaccagaa acgttgccgc taaccgtgcg actggggatc aataatgcgc aggccattcg
     4981 cgacgtattg ttaaacagta gcgaacaggc gctgagcgat cagcaaaatc aacagttaac
     5041 gcaatcattt tgcgatgtgg tggatgctat tattgctggt ggtgggatgg tcggtggtct
     5101 gggcgatcgt tttacgcgtg tggcggcggc tcatgccgtg cataacggtc tgactgtcct
     5161 gccgcaaacc gagaaatttc tccacggcac caaagtcgct tatggcattc tggtacaaag
     5221 cgccttgcta ggtcaggatg atgtgctggc gcaattaacc ggggcgtatc agcgttttca
     5281 tctgccgacc acgctggcgg agctggaaat ggatatcaat aatcatgcgg agatcgacaa
     5341 agtgattacc cacaccctgc gtccggtgga atacattcat tacctgccag tcacgctgac
     5401 accagatacg ttgcgtgcag cgttcgaaaa agtggaatcg tttaaagcct gacgtgcaaa
     5461 tttagcagca gcgcgcgccg ccttttccac cgtagcgcgc gtcctgccgc tcacggaaaa
     5521 actcttcgta ggtcatcggc gtttgatcgg gatggttaac ccgcatatgt tcgacatagt
     5581 tgtcgtaatc aggcatacca atcatcagct tcgccgcctg acctaaatat tttccggctt
     5641 ttgccagtga atcaaacatc ttggcctccg ctaaccatgt tgctgaaaga gtgccggatg
     5701 cggcgtgaac gccttatccg gcctacaaat gctatcaacg cgctcggtca gtcccctcgc
     5761 cccatcgggg agagggttag ggtgaggagg aaccctcggc actatgccaa cattcgccaa
     5821 catcccccct cactctgact ttagtgtgcg ccttttgcct gcgccacgat ctcctcgaca
     5881 ttttccggca ttggctcata cggcgtttct ttcgccgttg gcttcggatc tttcaatgcc
     5941 gccagtgccg acttaatcga gaacagcgcc agaaccacca cgaccaccat aaagaagatg
     6001 gttaacccgg catccagacg gttgttgaac accagttgcg ccagctgtga ctcagtatac
     6061 tgcgacggaa tattgccgct gtcgatcatt gcctggaact tattagcaat ggccaggaag
     6121 ccgactttcg catccgggct aaacgctttc tgccagcctg cggtcagggt acaaatcagc
     6181 agccaggccg ttggtaccag cgccacccag gcgtaacgtt gacgtttcat cttgaacaac
     6241 accacggcac agagcatcag cgccatccct gccagcatct ggttggcaat accaaacagc
     6301 ggccacagag tgttaatgcc gcccagcgga tcgaccacgc cctgatggag gaagtagccc
     6361 cacgccagta cgcacagcgc tgttgccagc aggttagcag gcagtgaatc ggtccgcttc
     6421 aggccaggag acaccacgcc cagcagatcc tgcaacataa agcgcgcagc acgcgtaccc
     6481 gcatctaccg ccgtcagaat aaacagagct tcaaacagaa tggcgaagtg ataccagaac
     6541 gccacatcca tcataccacc cagcgcaccg tgcaggatgt aagccatccc caccgccagc
     6601 gtcggcgcac cgcccgcacg ggaaatgatc gactgttcac ccacttcgct ggcaatctgg
     6661 ttgagcgtat ccggagtaat cgcaaagccc cagctactca ccacctgcgc ggcagaagcg
     6721 accacatccg ccgtccctgc cggagccagc accgccatcg ggctgttcat ggcgaagtac
     6781 acgcccggat cgatgataca ggcggaaacc agtgccataa tcgccacgaa ggattccatt
     6841 aacattccac cgtagccgat aaagcacgcc tgcccttcgt tcgccagcat cttcggcgtg
     6901 gtgccggaag agatcagcgc atggaagcct gacaccgcgc cacaggcgat ggtgataaac
     6961 aggaacggga acagattacc ggtccatacc gggccagtgc catcgacaaa tttggtcagc
     7021 gcaggcatgg tcagcgtcgg acgcataatc aaaatgccta ccgccagacc aacgatagtc
     7081 ccgattttca ggaaggtaga gaggtagtca cgcggggcca gcagtaacca caccggcagc
     7141 accgccgcca caaaaccgta gcccaccagc atccaggtca gctgcacgcc ggtaaagtca
     7201 aagtacggtg cccaggtcgg gctttctgcc acccagccgc cagagataat ggcaaaaata
     7261 aggaacacca ggccaatcac tgacacttcg ccaatacgcc ccggacgcag atagcgcagg
     7321 tagattccca taaaaatcgc cagcggaatg gtgaacgcaa cggtatacgt tccccacggg
     7381 ctatgagtca gggctttcac cacgatcatc gccagtactg caaggataat gaccatgatc
     7441 ataaagcagg ctaccagcgc aatcaccccg gcagttggcc ccatctcttc tttgaccagc
     7501 tcacccagcg agcgaccgtc acggcgcgta gaaacaaaca gcaccatgaa atcctgcacc
     7561 gcaccggcga gaaccacccc ggcgagcagc cagatcatcc ccggcaggta gcccatttgc
     7621 gctgccagca ccggccccac cagcggccct gctccggcaa tggccgcaaa atggtgaccg
     7681 aacagcactt tcttgtccgt cggcacatag tccagcccgt cgttatggcg caccgctggc
     7741 gtcatacgcg tcgggtcaac cgccagcaca tttttggcga tatacagccc ataaaaacgg
     7801 taagcgatca gatagataca gaccgacgcc accacaatcc acagcgcgtt gatctgttcc
     7861 ccacgattta aagcaatgta tcccagagca aatgctccca ttacagagag cactgtccag
     7921 acgaggtatt tccctgattt gttcatagtt gttatccgtg tgcgtgtcca tagagatgtt
     7981 acattttgtt tctataacat ctttgtacga tttaacaaca cggtaaccat gtaaatcgga
     8041 gttgaaacca atatttaact taacaatgtt aactcgatca ctccgttaac cgagttttcc
     8101 tgcaatctca tttaattctg tcgggctcat cccaaaactg ccgtacccag ccgacaagtg
     8161 cagcaacgcc gcccctgttc atcgaaaacg atgatttccc agctctgatt ttgccgccca
     8221 agatgcagcg gctggcagac gccgcgtacc ttgccttcag acaccgggcg atgatgggtg
     8281 gcgttaagtt ccgtacccac cacacactgc ccgtcacggg tcatcataaa tccggccatc
     8341 gatcccagcg tttcagccag cgccgccgac gcgccgccat gcagcaggcc aaacggctga
     8401 tgagtacggg tatcaaccgg catttcggct tccagcacat catctcccag acgggtatac
     8461 acaattccca gatgcgccac cattgtgtta tcgctggtgg cgttcagttc gtcgagcgtt
     8521 aaatggcgtt tccagatcat gcttatgccc ccagcgttga gccgccatcg accacaatat
     8581 cctgtagggt gatatggctg gcgaggtcag aggcgaggaa caaaatcgtg ttggcgatct
     8641 cttgtggacg ggcgattttc cccagcggaa tgccgagttt aaactgctcg ccaaagccgc
     8701 gaatacgctg ttcttcggcg tcatcgctca cccacagcgt gcgttgcata tcggtgtcgg
     8761 tggagccagg cgaaaccaca ttacagcgca cgccgctaac cgccagttcc agcccgacgc
     8821 tcaacgccag gcttttcagc gccgctttcg atgcgccata agcactcata ccaatacgcg
     8881 gcgtgtgtgc ggcgtcggac gccacagtga caatcgcccc gccccgctga cggcgaaact
     8941 ggttcatggt ttgctgaaac aggttaaacg caccgccgac gttaaccgca aaagtctgct
     9001 gccagtcctc tttactgagc tgatcggtcg cgcccatgcg caaaattccc gccgcattga
     9061 ccagcacgtc cagtcgctcc gtttcagcta acagtcgctg acatacctgc gcgacctgcg
     9121 cagcatcggc aacatccatc acttcggtcg caaagggata ttgctcctga gcgaacgctt
     9181 gatcaaaacc ggtaactttc gctcccgctt caacaaacgc cagcgccgtg gcgtagccga
     9241 tacctttacc tgcgccggtt acccagacat ttttaccgct gaaatccatt atttcacctc
     9301 gcgggagagt agcttccacc aggcgtcgat ggtcgggttt ttcgccagca tgacaaagtc
     9361 gatatcgcca tgcactttgc gccagcgcgc cgccagcgcc atcatgcgca ccgaatccag
     9421 accgtagtcg atcaggttgt cgtcatcgaa cggttcatcg gactcgtcca gcaacggcag
     9481 gataacctca cgcagcgccg ctttcctggc ggggacaggt gctggcagta atccttcagt
     9541 catcaccacc cggccagacc gcccggccac atatttcagc gacatcaaat gctcgtcacg
     9601 gctgaaatcg gccagcgcat ccgccaccat aaacggttta atatcgcgca taaatgcgtc
     9661 ggttgcggtg gtcatacagc caatgtgggc atataccccg gtaataatca gctggttacg
     9721 tccactctct ttgagcattt gctccagcgg agaacgatga aacgcgctgt agcgccactt
     9781 caccagcacc gtgtcgtcgg catctggcgt caggcgatcc accacctttt gctgttccgg
     9841 cgaacgggtc aggcccggcc cccacatatc attcaacagc gcccgatctt catcgctctg
     9901 ctctttcggc tgggcggtgt aataaaccgg gatattgtgc tgtttgcagt agtcgcgcag
     9961 cgcagcaata ttcgcgatca cctgctccat catcgggcag ttctcgcccc agaagctgac
    10021 aaaatagtcc tgcatatcat ggattaacaa cgcggcacgt tgcggttcaa aggcccagtc
    10081 aactttattc tgcggaatat cgtgagactc cggcagtgcg taagcctgta attttggaat
    10141 agccatcgtg ttctctcctt caggctgatg cgcgtgacgc cagccactga cgtaattgtt
    10201 ttttatcgac tttcccgacc gccgtcagcg gaagtgaatc cacacactcc acgcgatccg
    10261 gtaatttaaa ttcggcaata ccctgttcac gcaggaaacg acgcacctgc accgcgcgca
    10321 gcggctcttt taccaccaga taagcacagc ttttttcgcc catcagctca tcttccatgc
    10381 tcaccagtgc ggcatagatc accgccggat ggcgcagcag caggttttcg atctcttcgg
    10441 cagcgatctt ctcgccgcca cggttgatct gatctttctc gcgcccctgc acggtgatgt
    10501 aaccctctgg atcaatagag atcagatcgc cggaacagta aaaaccgttg gcatcaaagg
    10561 cactggcatt gtgctgcgga cttttgtaat aaccgcggaa ggtgtacggc ccgcgcgtca
    10621 tcaggcgtcc aacttcccct tgcggcagtg gatttccttc agcatcagca acccatactt
    10681 catcgtccgg acacattggg taaccctggg tatggataat tttctccgcg ctatcatcaa
    10741 gtcgggtgta gttcaccagc ccttccgcca tgccaaacac ctgctgcaac tggcagccaa
    10801 tctcagcgga aatacgcgcc gcaagggtgg cagaaagacg tgcgccgccg acctgtaaca
    10861 gtttcagcga ggcaagctgc gcccggcttt cgccttcagc cagcgcctgc aaccacaggc
    10921 tgactgccgg cggcaccagc gcggtaacgt taacctgatg ttttgcaatc aacgggaagc
    10981 aaagcgtggc gctgggatcg gccgccagaa caaccgttcc tccggcaaga aagacgccca
    11041 gcgatcctgg cgaactcata gcgtagttat gagccgccgg gatcgcgcac aggtagcgtg
    11101 tctgttgtgt gaactgacaa atctcgacgc tacgacgcac gctgtagtag tagtcgttat
    11161 gagtgcgcgg gatcagcttc ggtgtaccgg tggtgccgcc ggaaagctgg aaataggcca
    11221 cttcatcagc aggtgatggc gtagcagtaa aaccgtctgc cggatggtta atcgcatcct
    11281 gcaagttatg ctcaccgctg tcgttgagca gttgcaccac gcgaatggag gaatgttctg
    11341 tgacgaaagt attgaggaaa tcatccccgc taaacagcgc atgttggcga tcggcaatca
    11401 gtaacgccgg ttcaatctgg ctggcatagg cgttcagttc actacgctga tggctgaaca
    11461 acgccagcac cggcgcaacg cccagtttca gcagtgcgaa aaaggtaatg taaagttcag
    11521 cgacgttacc cagttgtacc agcgcggttt caccgggttt aatgccctga cggcgtaaac
    11581 tacacgcgag gttatccgcc gcctgattca gctcccggta actcaactgt cgctcgccgt
    11641 cgataaccgc gatgctgtca ctcgcagcgt ggcgagtcag aatgtcggtc agcggcaaat
    11701 cctgccagta gcctttttcc cgatagcgac gggcaaactc ttccggccag cgggtgaatg
    11761 gaatgctcat cctcgctcct taatgcaatc caaaaacgtt caacatggta gaaagtttga
    11821 cgcctgtttc gcgccactca cccaacggtg acgacgcagg cacaatcccc gctccggcaa
    11881 acagacgcac ctgattttcc cgcagcttcg cgcagcggat ggtcaccacc cattcgccgt
    11941 taccttcgct gtcacaccaa cccacaatgc cgccaaacag ttcgcggtca aacggctcca
    12001 gttcagcaat aacctgggtc gcggcctgat gcgggaaacc gctcagcgcg ggggtcggat
    12061 gcagcagaca ggccagagtc agtgcgtttt cttgcgaatt cgctttacct tcaaagggag
    12121 ttgcgagatg ccacagcgtc ggcgtggtaa tcaattgtgg agaggaggga acgtgtaact
    12181 cactactgcg ttcgcgcagt acctctttca tcgcctgagt caccagttca tgttcatggc
    12241 gatctttttc tgacgccagc agacgattac ccgcttcgcg atcgagcact tcatccggct
    12301 gacgacgcgc ggaaccggct aacggaatgg agctaaaacg ctcgccgtct ttacgtagca
    12361 gcagttccgg gctggccccc agcaggacgc caccatcagc cagcgggaca tggaagttgt
    12421 aactaaccgg gttttgcgca atcaaccgtt ccagcaatac gccactatca atggcggcgt
    12481 cagtggtgat atcaatcaat cgtgacaaca ccactttgtc gacctgcggc gtggcggtaa
    12541 gtgcggcggc gcgggcaacc atctgttcaa acgtggtttg ttcaggaatt gcctggcgtt
    12601 ccaccacgtt cagcgactgg ctgcgggtaa aacagtgggc tgaggtctgt ttttcctgac
    12661 gggagaacga ctgccaggat tcgggaatat acagcgacga aggctgacgt ggatcgaagg
    12721 gaatcgcccc gaccatcacc ggatttttga tgccctgcgc tttggcatcg gcaaacagcg
    12781 cggcgagttt ttgctggaag ggactgtcgg gcgaatcccc gttcacagcc ggttcatcga
    12841 agcgggcgaa acatcctgac gtcgtaaaac tgcggtacgg cgacataaag aaaaagcgat
    12901 tgggcgcaag tgttgccatg gtctgctgta cttcctcagc cagtgacgta tccatatcat
    12961 cctccacaaa atgataaagg ctttaataat gattatcatt tatattttcg gtcgctaacc
    13021 taaaagcaaa cgcgcactat gtcaactctt gaggtaacgc aatctgcaac tcggggttgc
    13081 atctggttgt ggttacaatg aaaatgagaa gcattattga tgaattcgca taagcgcaat
    13141 gtgatgtcct gcgccgtttt gccccctctc ccttccaggg tgagggctgg ggtgagggtt
    13201 aatgttcgca ccagtgctgg ctgttcccct caccctaacc ctctccccaa aggggcgagg
    13261 ggacggattg tgcgctttgt cgaatttgtc attacgccct taaccttatt aataacagga
    13321 agctgacttg tgagactcgc cccgctctac cgcaacgccc ttctattaac aggacttttg
    13381 ctttcaggaa tagccgtagt tcaggccgcc gactggccgc gtcagattac tgacagccgg
    13441 ggcactcata ccctggaaag ccagccgcag cgtattgttt ccaccagcgt caccctgacc
    13501 ggatcactgc tggcgattga tgctccggtg atcgccagcg gcgcgaccac gccgaataac
    13561 cgcgtcgcag atgaccaggg ctttttacgc cagtggagca aggtggcgaa agaacgcaaa
    13621 ctgcaacggc tctatatcgg cgaaccgagc gccgaagccg ttgccgcgca aatgccggat
    13681 ctgattttaa ttagcgcaac cggcggggat tcggcgctgg cgctgtatga tcagctttcc
    13741 accatcgccc cgacattaat catcaattac gacgacaaaa gctggcagtc gctgttaacg
    13801 cagcttggcg aaattaccgg gcatgagaaa caagcggcag agcggattgc acagtttgat
    13861 aagcaactgg cagcggcgaa agagcaaatc aaattaccgc cgcagccggt cactgccatt
    13921 gtctataccg ccgctgcaca cagtgccaat ctctggacgc cggaatcagc acaagggcag
    13981 atgctggaac aactcggctt tacgctggcg aagttgcccg cagggttaaa cgccagccaa
    14041 agccagggca aacgccatga catcattcag cttggtgggg aaaatctggc tgctgggtta
    14101 aacggtgaat cgctattcct gttcgccggt gatcagaaag acgccgatgc gatttatgcc
    14161 aatccactgc tcgcgcacct gcctgcagta caaaacaagc aggtttatac gctgggaacc
    14221 gagacgttcc gcctggatta ctacagcgcc atgcaagtgc tggacagact taattcactg
    14281 ttaaaaaaag attgataaaa catacaatta gagcactaat aatcccctcg cccctttggg
    14341 gagagggtta gggtgagggg aaaatgcacc tttagctaac tgtcagacgc tgtcacctgc
    14401 ggcggcgtct ggcgaaaacg tcgcaactcc accagcacca gcaataacaa cacgccgata
    14461 atcaacaaac caaaaccgct cgcgcttgcg gaagcaaccg gtgtcatcat cgcgcccaaa
    14521 ccacccaaca gcgccgcgcc tatggcatcg cccgtcacgt tctgcgccgt ccacaaaccg
    14581 ttaatccgcc ctaacatcgc ttccggggtt tgcgtttgca gcattgtata ttgcagcaac
    14641 gaactaaccg cgctcagcca gccgaacagc gccagacaaa ccacgcctaa aatccacatc
    14701 ggcatcaggc caaacagacc aatggcgagg aacgatccca gcgtggagag cagcatcaat
    14761 aaccctggtc gcgcactatg tgccagcttc ccgctggtta acgcaccaat agccgcaccg
    14821 agcgggatcg ccgcatagag aaaaccaatc tgtgccgccg acatctgcca gttgtcagcc
    14881 agcgccggat acagtacccg caccgcgctc gccatcgtca ataaaccacc cagcaacgca
    14941 atccctccta ccagcgggct ggcgagcaga aaacgaaatc ctgccagtaa tgatttcaac
    15001 ggatgctcgc gcggctgcgg tggcggtggc aacgccggaa ggcttaacaa cggtagcaag
    15061 gtaataaacg tgcccgccgc cgccagcccg tagttccagg ctacgccgcc ggtcgccagc
    15121 aataaaccgc caatcatggg cgaaatcacc gaccccagac gcacggtcaa catggtgatc
    15181 gccccggcct gcattaagtt ttcacgccct accagtgcag gtgttgccgc cagtagcgcc
    15241 gtaacaccaa gtgatgcgaa aaaaccatcc cataaaccaa gtaaatagat tgccagcaat
    15301 gacggctccg gcagcagcgc gttcaggcac agtccaatga agccaatgcc acaggtgccg
    15361 cgcgccagca aaatcacttt tttgcgctca tagcgatccg ccagcacacc gccaaccatc
    15421 aggccaacaa acatcgcgcc gccggtcagc gtcaccgaaa gccccacctg ccaggtagaa
    15481 tgcgtcatca tctggatctg caccggcacc gcgacgccga gcaaacccag agacacaatt
    15541 gagataaaac gagcgaggaa tactgcgcga aacgccgggt gcgttttcaa caggctgagg
    15601 ttaagcagcc aggattgttt attcattaca atgccttgcc atcaaatatt ttaaatacct
    15661 attcttaggc tgcacatgct aacatatcca aataagatcg ataacgataa ttaatttcat
    15721 tatcatggaa gttcgtatgt ctggttctgt tgccgtgaca cgcgccattg ccgtgcccgg
    15781 attgctgtta ttactgatta tcgcgacggc attaagcctg ctcattgggg caaaatcact
    15841 ccccgcgtca gtagtgctgg aggccttctc aggcacctgc cagagcgccg actgcaccat
    15901 cgtgctcgac gcgcgactgc cgcgcaccct tgccggttta cttgcaggcg gtgcgcttgg
    15961 gcttgctggg gcgttaatgc aaaccctcac ccgaaatcca cttgccgacc ccggcttgct
    16021 tggcgtgaac gccggagcca gctttgccat tgtgctgggt gcggcgctgt ttggttactc
    16081 atccgcgcag gaacaactgg cgatggcctt cgccggggcg ctggtggcct cattgattgt
    16141 tgcctttatc ggcagtcagg gcggcgggca gttaagtccg gtgcgtttaa ccctggcggg
    16201 cgtggcgctg gcggcggtgc tggaagggct gaccagcggc atcgccctgc ttaatcctga
    16261 cgtctacgat caactgcgtt tctggcaagc cggttcgctg gatattcgca atctacatac
    16321 cttaaaagtg gtgctgatcc cggttctgat cgccggagca actgcgctat tactgagtcg
    16381 cgcgctgaac agtttgagcc tcggcagtga caccgcgacg gcgctgggca gtcgcgtggc
    16441 gcgcacacag ttgattggtc tgctggcgat taccgtgctt tgtggtagtg cgacggcggt
    16501 agttggcccg attgccttta tcggcctgat gatgccgcac atggcgcgct ggctggtagg
    16561 tgccgatcat cgctggtcgc tgcccgtcac gctacttgct acccctgccc tgctgctgtt
    16621 tgccgatatc atcgggcggg tgattgtgcc cggcgaactg cgcgtttctg tggtcagtgc
    16681 gtttattggt gcaccggtgc tgatcttcct cgtgcgacgt aaaacgcgag gtggtgcatg
    16741 atttacgtct ctcgccgatt actcatcacc tgtttgctgc tgatttccgc ctgtgtggtt
    16801 gcaggtatct ggggattacg cagcggtgcc gtcacgctgg aaatctcgca ggtattcgcc
    16861 gcgttgatgg gcgatgcgcc gcgcagtatg acgatggtgg tcaccgaatg gcgtttacca
    16921 cgcgtgctga tggcgctgtt gattggcgcg gcgctgggcg tcagcggcgc gatttttcag
    16981 tcgctgatgc gtaacccgct cggcagccct gacgtaatgg gctttaacac cggggcgtgg
    17041 agcggcgtgc tggtggcgat ggtgctgttt ggtcaggacc tgacggctat cgcgctggca
    17101 gcaatggtgg gcggcattgt cacttctctg ttggtctggc tgctcgcctg gcgtaacggt
    17161 atcgacacct ttcggttgat tattatcggc atcggcgttc gcgccatgct ggtggccttt
    17221 aatacttggc tgttgctgaa agcgtcttta gaaacggcgc taacagcggg tttgtggaat
    17281 gccggatcgc tcaatggcct gacgtgggca aaaacttcgc cttccgcacc catcatcata
    17341 ttgatgctca ttgccgccgc cttgctggtg cggcgaatgc gcttgctgga gatgggcgat
    17401 gataccgcct gcgcgctggg cgtcagcgtc gagcgttcgc gtctgttaat gatgctggtt
    17461 gcagtggtgc ttaccgctgc ggcaacggcg cttgccgggc cgatttcctt tattgcttta
    17521 gtcgcgccgc acattgcccg acgcattagc ggcaccgctc gctgggggct gacccaggcg
    17581 gcgctgtgcg gagcgctgtt actgctggcg gccgatctct gcgcccaaca actgtttatg
    17641 ccgtatcaac ttccggttgg cgtcgttacc gtcagcctcg gcggtattta ccttatcgtc
    17701 ttgttaattc aggagtctcg caaaaaatga ccgaatcagt agcccgtttg cgcggcgaac
    17761 agttaaccct aggatatggc aaatataccg ttgcggaaaa tctgactgta gaaattcctg
    17821 atggtcactt cacggcaatt atcggaccaa atggctgtgg taaatccacg ttactgcgta
    17881 ccttaagccg cctgatgacg cctgctcacg ggcatgtctg gctggatggc gagcacattc
    17941 aacattacgc cagtaaagaa gttgcacgcc ggattggtct gttggcgcaa aatgctacca
    18001 cgccgggcga tatcaccgtg caggagctgg tggcgcgtgg acgttatccg catcaaccgc
    18061 tgtttacccg ctggcgcaaa gaggatgaag aagcggtaac gaaagcgatg caggccacga
    18121 gaataactca tctggcagat caaagcgtgg ataccctttc tggcggacaa cgccagcgag
    18181 cgtggatcgc gatggtgcta gcccaggaaa cggcaattat gctgctcgac gaacccacga
    18241 cctggctgga tatcagtcat cagattgatc tactggaact gttaagcgaa ctgaaccgcg
    18301 agaaaggcta taccctggcg gcggtgctgc acgatcttaa tcaggcctgt cgttacgcca
    18361 gccatttgat tgcattgcgg gaagggaaaa ttgttgctca gggagcgccg aaggagattg
    18421 tcactgctga actgattgag cgcatttatg gtctgcgatg catgatcatt gacgatccgg
    18481 tagccggaac gccgcttgtg gtgccgctcg gacgaacggc accttcaacc gcaaatagtt
    18541 aaactaagtg gtctgccatc atggcatcct gttttctgga tgccatcgca tagcgcaata
    18601 acacgctacc acaagccacc atcccgccga tcagcgcgga aaggatcaca ataatcgcct
    18661 tacccggacc gtcttttttc actggcaatg acgggcttaa ctgatattta aacggcgtaa
    18721 aattcacatc gttgacattt gcttttgtta attgctcgac aagatactgc cgattacgta
    18781 attcaccgtt cagttccgca acgtcagtaa ccgctttttc aatttccagt ttgcgttcaa
    18841 taccgtctgc gccgagagaa atagagaaat cggggtcatc tttaacggcc tgaccattac
    18901 tgtatacggg ctttttaatt cctgccgcgt tggcaatgtc gagtgaataa ttgaggcgct
    18961 gaatgtttgc atcaagttga tttttcgttt taatgcgatc ctgagccagt ttttcttttt
    19021 caaactgggt tttgatctcc agtttattac ggacgttttc tatcgactct ttcaccacca
    19081 acgcagagat ataatcgata tacccgctca aaacggtctg cgcctcttta ctggttggcg
    19141 cggtaaaact tagcgtccag gaggtataca gtgacggttc atcttttttc ttactggcat
    19201 tgtcatcaac cgctttcatt ttttcgctca atgcaacaat agcgcgatgc aaatccagtt
    19261 catcgatttt cgcctctttt aattggtcca tcacataagg tgatgaacgc aggtactctt
    19321 ccagcaagct aacagactga aacttcttga taaacaggtt aaatgcaccg ccacggtcga
    19381 tattaacatc gagatcaaga acgcggagtt tggtaaaggt tttctccagg tcctgccact
    19441 gaatggcttc ggcgggagta ataacggctg agctggtcca tttttgcggc aggatgaaag
    19501 agatcagtaa acctgcgcag gcaaatgcaa aaacgaccgc catgaccgtt tttttggccc
    19561 gccataaaac ttcgattaga ttaagtaaat caatttcatt attactgggc gacgccagag
    19621 gatactcaga aaaatgagcg tcacttccct gtttaatatt caatgatgac atggggtata
    19681 acctgaaaat gagtccaata ggggaaaatc tccacggcgc tcactttacc agttggtgag
    19741 aattatccga agctgaagtt tgtaaattcc ttccactgaa tgcaatattc actctattta
    19801 agagaaatat tcatcaaacc tataaatcaa acaaataaaa aacccataat tattttctgt
    19861 aattatgggt ttatagataa tattaattta cctgtttagc gttgcgcgaa taatcggccc
    19921 aatttttaca aacgcccctg gagagataat atccacatgc gcacaatcct gacgatagat
    19981 atccagctcc gcaatccacg gcgaccaggc gcgttcagga ctcatacctt cctgaagcgt
    20041 gcgttcagca acaaacaacg tcgctttacc gtcaaacggt acgctatgag cagtcgtcag
    20101 caggcgcaca gcatcagcgt agttgccttc aatggtggta aacaactccg ttgaagtact
    20161 tccctgctgt gccgccagga aggcctcgcg ctcgcggtta atctccgcca gcacttccgg
    20221 gtccagacca ttagcttctt tttcctgcca gttttgcgtt tctggcggcc aggtatccag
    20281 caagccaaga aatgccacct gttcgccacg ggcacgcagt cgcgccgcaa taccctgcgc
    20341 cagcgtaccg ccaagggaat accccagcag gtaataaggg ccgtgcggtt gttgctcaag
    20401 taacgttgcc agatgcgctt cgcagacttc atccaggttt gtcgctgtct gcatgggtcc
    20461 atgagggcgc ggcgactgaa tgccaataat cgaccattgt ggatcgagat aacgcgagag
    20521 tacgctaaac tgccaggcaa aaccggacgc cggatggaaa caaaacagcg tcgggccatt
    20581 accttcacgc aacggcagaa tggtttcgaa tcccatgcgc cgggagctgt cctcttcacc
    20641 atcaataatc gttgccagtt tggcgacggt tgacgcgacc atcacctgcc ccggcgtcac
    20701 ctgacgagca aactgtcgac ttaactgcgc tgccagtttc attgccagta gcgaatgacc
    20761 gcccagcgca aagaaatcag catcggcatc ctgcacgtca caacccagca acgacgcgaa
    20821 tgccgcggcg ataatcgttt cactgcccgc tttcggtgca cgtcccgacg cttgtgtctt
    20881 cagttcaggc aacggtaagg ctttgcgatc cagcttgccg ttggcgctaa gaggtaactg
    20941 tggaagttgc agcagaacca ccggtaccat atgtggtggc aatgtttcac gaagctgcgc
    21001 ctgcaatgcg ctggtatcca acggcaggcc cgattgcgac accagatagc ccaccaactg
    21061 acgcgcatca ccaccagtgg ctgccgcctg gttaatcaca caggcgtggg taacggcttg
    21121 ttcgacatcc ggcagcgcct gcatcacgcg atcgatttcg cccagttcga tacgctgccc
    21181 gcgaattttt agctgatcgt cactgcgccc gaggtactcc accgcgccgt tatccagcca
    21241 gcgggcaacg tctccggtac ggtacatccg ttcgccagga gcaaaaggat cggcaataaa
    21301 gcggctggcg gtcagatcgg ggcgtccgag atagccctgc gccagttgaa tgccggtaag
    21361 atagagatca cccgccacac ccggcggcac cggatgcatc atcgcatcaa gaatacgcag
    21421 ccccatgttc cacaccggat aaccaatcgg cacactgctg ccgcgcacct gtgccagttc
    21481 ctcgccaaaa gccggatacc agctgacatc taccgccgct tccgtcgggc catacagatt
    21541 atgtaacggc gcaccggtta actgttgcca ttcacggcat aaatcagccg gtaaggcttc
    21601 accactacag aaaacctgtt tcaacgtcgc gcaactctgg cgagcggttt gcggagtcag
    21661 cgaggcaaca aatgccgcca gcatcgacgg cacaaagtgc gtggtcgtta cgccatattc
    21721 ggcaaagaat tgctgcatag cgagcgggtc acggtgcgct tccggttcag ccatcaccag
    21781 ttttgcccct gcgataaacg gccagaaaaa ctcccacacc gagacatcaa aactgcacgg
    21841 cgttttttgg gcaacgacat cttcgcctgt aagcgggtag tgattttgca tccataacag
    21901 gcggttaacg atagccgtct gcccgaccat tacccctttc ggcctgccgg tggagccaga
    21961 ggtaaagatg atataagccg tgtggtgcgg ttgtgaaagt tgcagcggcg cactgccctg
    22021 gggggtaagc ggggcgttat agcaaaggct tgttaaattg ggaacatcgc taaagcgcgg
    22081 cagttgatcg tcggtggtaa ttaacagcga cggacgcgca tcttccagca tcattttcag
    22141 gcgatcgtcc ggatagccgg tatccagcgg tagccaggcc gcgccagctt caactattgc
    22201 atggagtgcc agggtcaaaa agaccgagcg cggtagtgcc accgccacgc tgtcccctgg
    22261 tttaacgccg cgctcacgca gcagattcgc cagcgccacc acctgctcgc gcatttcccg
    22321 atagctgaac aggtaacgcg catctgccag cgccggagca tccggtgttt ttgccgcttg
    22381 ttctgccacc agcgcgctca gcgtggtttc tggaatctca acctgagtgg cgttgatctg
    22441 cgccagctgc gcatactcac ctggcagcat aatatcgaca tcgccgcaca acagcgccgg
    22501 atccgcggcg aactgggcaa tcagcatttt caggcgttca gcatgctgga ttaacgttgg
    22561 ctcatcgtaa cgctgtttat tggcgaggat ctcaatactc aaatcaccgt gttcatccgg
    22621 gaacagggcc agttcaaggt cattaaccgg accggttgcc agggtatggg tttgcgcctg
    22681 aacaccagga atatccagtt ggtaatcaaa taccttaata ttgagtaccg gaccaaacag
    22741 cggttcatca cctgccgctc gcccgctgtc acggacaatt tgttcggcat cgtaacgttg
    22801 atggcgacgc atttttttca gttgagccgc cagtcgggtt gccagttccg gcagcgtttc
    22861 ttgcgccgca atgtgaatac ccaacggtaa aacgttgagc acgggtccgc tagccgtcag
    22921 cgccgccgag cccagtcgac gcataaagat aaatccggcg gcatagtcca tgcgattgca
    22981 caatcgcccc agccacaagg ctgccagcgc aagggctaaa tcggtacgct gcacacctga
    23041 aagttgcgta gccagctggc ggaattcccc gtcggtaaat tccagtttca ggcgcagaat
    23101 atctgccgaa gcgctgcgcc ccggtaaagg tgccggagaa agtgacgcgg gcggcggcag
    23161 ttgacgacgc tgttctgccc agaatgccgc atcacgctgc caggcttcgc tttcgcggta
    23221 ttgctggtac tcttccacta catcagcgaa aggcgtaaac ggcgaagcag gcgttggttc
    23281 gccacgcagc catgtgcagt aaatattggc gatctggcga gtaatggccg ggaaactgaa
    23341 gccatcgacc agcaaatggt gataacgctg ataccagtac cagcggttat ccgcaacatg
    23401 tatcagctga tgaaagacca gtggtttacc gctatcgacg cgcaaatctt gttgcaaatc
    23461 cgccagcatt aatgcctgcg cagtaccgtg cggatcaata ttggttcgca ggtcgataat
    23521 ttctggctgt tcgaatatca gcgcatcatc gacccactgc cagacttcgc cgttatcttc
    23581 cgtaaaacgc atccgcaacg tatctgcttg cgctagtccg gcaaccaccg cgcgggccag
    23641 taatggcgca tcaacatctc cagttaactc aacgtaatgc gccacgctcc aggcggaggg
    23701 taaatccgac agtttttctg ccatccagat gccgggctgt gcggcgacca aaggtaaatg
    23761 ctggctcatt gtgcctcctg caactgggtg aaattggccg gtgtcagagt acgccagtgg
    23821 acttccagcc actgctggca ggacgcctgt gactgcggct gacacacaat gtcccagcct
    23881 gccgataaga ggcattgttg cggccacaga ctgaattgcc cctgcgcatt gcgcaaaatg
    23941 taaaacgctc cctgcggatc atcgaaggga ttactgaatg ccatactcaa ctcctgtcat
    24001 ggaaaagtgg ttgccagagg tcgattaacc cctgcatcaa gccaccgcgc caacaaagcg
    24061 catcatgtcc gccgtcaacc tgacgccaga aaatggattc ttttataggg tgcaattgcg
    24121 catacagcgc ctgattggcc cgcatgatca tcggctcgcg aatacccgct tccagcacaa
    24181 tgcgcagacc ttcggcgcta acttcaccag cttttagctt ttcaagtaac acgccctctt
    24241 gctgcccgtc ccgatgcggc caccagtacg atcctgactg gcttaataca cagccaaagc
    24301 gttcaggcca gtgcagtccg gcatacagcg cggacagccc accaaaactc tgcccggcga
    24361 ccacggtgcg atcggcgcga tcgctaaaag gggcaatagc tttcaccagg ggtaataact
    24421 cttgctgtac tgccagccag aaatccgcat tacacggcag ttcgtgggcg cggtgcgtgg
    24481 tgtcgatagc gtcgatcaac acatacacgg cgggaggaag ttgctgacga tgggtcagcg
    24541 aagtcagcac aggccagacg ggcatacttt gcgcccaaaa ttcgccatcg agcaaaactg
    24601 ccagcggacg ttcttctgct gttacatcgc cggtggtaaa aatccataca cgacgtgaat
    24661 ttttcaaccg ttcacttttc cagataattt ctttggctgc tatttctggc acttgcggac
    24721 aatcccatcc cggttgcaga ggcgcttgcg gcatttcgag tgcagaaaca gcgtgccctc
    24781 gcccgccttt ccagctttgt ggattcagcg gatcggctat tgcctggggt aatagttttc
    24841 gccagccttc acgtaattcg aggcgatcgg gggatggtgc agaaaaaatg tcatcgcgtt
    24901 cggtgggaat aaagcagtag ctgccgcgcc agttggcatt gagttgtgtc gtccactgcc
    24961 agacatcagt acctgcaatt cgctgcatcg actggggctg gctgttctga tgatgatcgg
    25021 tcacaccagt gatgtagatc catacgcgct ttatcgtcga gtattcttca gaaccttggg
    25081 gatcacgcca ccagaaagtg acctcaaaca tttcgtcatt cagacgctgc cattccgggc
    25141 catgtttcga ctgccaccag ctctcacttc ctacttttaa cgccgtcaca ccataacccc
    25201 atgtttactg tgcaattttt cattgattgc agaaatatat tgataatatt attgataacc
    25261 atttgcattt gcaatagcgt aatggcgcgc cgtgggaagc gcggacattt attaaccaac
    25321 tgcactgcgt gtctttcagg atcaaaggtt ttcgcggtag cgggatgcgt cgtgttgagg
    25381 acgaccatgc ccgacagttg caattcgtgg caaaaatgca ggaataaaac aatgaacaag
    25441 aagattcatt ccctggcctt gttggtcaat ctggggattt atggggtagc gcaggcacaa
    25501 gagccgaccg atactcctgt ttcacatgac gataccattg tcgttaccgc cgccgagcag
    25561 aacttacagg cgcctggcgt ttcgaccatc accgcagatg aaatccgcaa aaaccccgtt
    25621 gcccgcgatg tgtcggagat catccgtacc atgccaggcg ttaacctgac cggtaactcc
    25681 accagtggtc agcgtgggaa taaccgacag attgatattc gcggtatggg tccggaaaac
    25741 acgctgattt tgattgacgg caagccggta agcagccgta actcggtgcg tcagggctgg
    25801 cgtggcgagc gcgatacccg tggtgatacc tcctgggtgc cacctgaaat gattgaacgt
    25861 attgaagttc tgcgtggtcc ggcagctgcg cgttatggca acggcgcggc gggcggcgtg
    25921 gttaacatca ttaccaaaaa aggcagcggt gagtggcacg gctcctggga cgcttatttc
    25981 aatgcaccag aacataaaga ggaaggtgcc actaaacgca ccaacttcag cctgaccggt
    26041 ccgctgggcg acgaattcag cttccgcttg tatggcaacc tcgacaaaac ccaggctgac
    26101 gcgtgggata tcaaccaggg ccatcagtcc gcgcgtgccg gaacgtatgc cacgacgtta
    26161 ccagccgggc gcgaaggggt gatcaacaaa gatattaatg gcgtggtgcg ctgggatttc
    26221 gcgccattgc aatcgctgga actggaagca ggttacagcc gccagggcaa cctgtatgcg
    26281 ggcgacaccc agaacaccaa ctccgattcc tatacccgct cgaaatatgg cgatgaaacc
    26341 aaccgtctgt atcgccagaa ctactcgctg acctggaacg gcggctggga taacggagtg
    26401 accaccagca actgggtgca gtatgaacac acccgtaact cgcgtattcc ggaaggtctg
    26461 gcaggcggta ccgaagggaa atttaacgaa aaagcgacac aggatttcgt cgatatcgat
    26521 cttgatgacg tgatgctgca cagcgaagtt aacctgccga ttgatttcct cgttaaccaa
    26581 acgctgacgc tgggtacgga gtggaatcag caacggatga aggacttgag ttccaatacc
    26641 caggcgctga ccggggcgaa caccggcggc gctattgatg gtgtgagtgc caccgaccgt
    26701 agcccgtatt caaaagcaga aattttctcg ctgtttgccg aaaacaacat ggagctgact
    26761 gacagcacca tcgtaacgcc ggggctgcgt ttcgatcatc acagtattgt cggcaataac
    26821 tggagcccgg cgctgaacat atcgcaaggt ttaggcgatg acttcacgct caaaatgggc
    26881 attgcccgtg cttataaagc accgagcctg taccagacta acccgaacta cattctctac
    26941 agtaaaggtc agggctgcta tgccagcgcg ggcggctgct atcagcaagg taacgatgac
    27001 ctgaaagcag aaaccagcat caacaaagag attggtctgg agttcaaacg cgacggctgg
    27061 ctggcgggcg tcacctggtt ccgtaacgat tatcgcaata agattgaagc gggctatgtg
    27121 gctgtagggc aaaacgcagt cggcaccgat ctctatcagt gggataacgt gccgaaagca
    27181 gtggttgaag gtctggaagg atcgttaaac gtaccagtta gcgaaatggt gatgtggacc
    27241 aataacatca cttatatgct gaagagtgaa aacaaaacca cgggcgaccg tttgtcgatc
    27301 atcccggagt acacgttgaa ctcaacgctg agctggcagg cgcgggaaga tttgtcgatg
    27361 caaacgacct tcacctggta cggtaagcag cagccgaaaa aatacaacta caaaggtcag
    27421 ccagcggttg gcccggaaac caaagaaatc agtccttaca gcattgtggg tctgagcgcg
    27481 acctgggatg tgacgaagaa tgtcagtctg accggcggcg tggacaacct gttcgacaaa
    27541 cgtttgtggc gtgcgggtaa tgcccagacc acgggcgatc tggcaggggc caactatatc
    27601 gcgggtgccg gggcgtatac ctataacgag ccgggacgta cgtggtacat gagcgtaaac
    27661 actcacttct gatgctaacg tcagattgtt gacaaagtgc gcgtcgttca tgccggatgc
    27721 ggcgtgaacg ccttatccgg cctacaaaaa tcttgccaat tcaatatatt gcaggaccat
    27781 gtaggcctga taagcgtagc gcatcaggca gttttgcgtt tgtcatcagt ctcgaatatg
    27841 gtcgatatga aaactacgca tacctccctc ccctttgccg gacatacgct gcattttgtt
    27901 gagttcgatc cggcgaattt ttgtgagcag gatttactct ggctgccgca ctacgcacaa
    27961 ttgcaacacg ctggacgtaa acgtaaaaca gagcatttag ccggacggat cgctgctgtt
    28021 tatgccttac gggaatatgg ctataaatgt gtgcccgcaa tcggcgagct acgccaacct
    28081 gtctggcctg cggaggtata cggcagtatt agccactgtg ggactacggc attagccgtg
    28141 gtatctcgtc aaccgattgg cattgatata gaagaaattt tttctgtaca aaccgcaaga
    28201 gaattgacag acaatattat tacaccagca gaacacgagc gactcgcgga ctgtggttta
    28261 gccttttctc tggcgctgac actggcattt tccgccaaag agagcgcatt taaggcatcg
    28321 gagagacaaa ctgaagcagg ttttctggat tatcagataa ttagctggaa taaacagcag
    28381 gtcatcattc atcgtgagaa tgagatgttt gctgtgcact ggcagataaa agaaaaaata
    28441 gtcataactc tgtgccaaca cgattaattg acaacatctg gtacgattcg cccgcagcca
    28501 tcactgactg cgggcgaaag tgtaaagcag gtgccttacc actctgacct gacaaccgga
    28561 tatgcaggga ttgctccccg cacaacggct acttcttcgg ttcgtaagcg agaacagcct
    28621 taaactcaat attacgttcc tttacggtga actcacacag cgagtctccg accagaagcg
    28681 taaatcccag caccgtcaaa cacagcacta tgactgccac aagggcatat ttcgtcagca
    28741 tgtttatatt gcctccatgc gaagaaggga ctaccatccg agttgttgag gttcggagtg
    28801 gcagccccag gattgataga aatatctcct ggggcttcat ctttccagac ctcagagtaa
    28861 acctgaaacc ggaaagcttc aggcacccgc cgttatctta cccttcctca gcaaaatatc
    28921 tgtttattta tacagtaagt cctgtttcgg acttctgttg gcaacactcg ttattgctcg
    28981 taaaacgaaa tcgcccgcag ccatcactga ccgcgggcga aagtgtaaag caggtgccct
    29041 accatcctga cctgacaacc ggatatgcgg ggattactcc ccgcacaacg gctacttctt
    29101 cggttcgtaa gcgagaacag ccttaaactc aatattacgt tccttcaccg taaactcaca
    29161 cagcgagtct ccgaccagaa gcgtaaatcc cagcaccgtc aaacacagca ctatgactgc
    29221 cgcaagggca tatttcgtca gcatgtttat attgcctcca tgcgaagaag ggactaccat
    29281 ccaaattgtt cgggtttgga gtggcagccc cagggttgat agaaatatct cctggggctt
    29341 catctttcca gacctcagag taaacctgaa accggaaagc ttcaggcacc cgccgttatc
    29401 ttacccttcc tcaacaaaat ggctgtttat ttatacagta aatcctgttt cggacttctc
    29461 actcctcatt taatcaactg aagctgagcc cgttttttct gcatgcctca atcgtcaaaa
    29521 ttcttcgcca tcagccagct ctgccataac ttgccacatt gaaaaagtag ccaaattgct
    29581 tcagtcacca tcatcaaaaa aagaaacatt ccagaacttc cgcatctcgc ccagccgctc
    29641 cgacttctat actgaataga aaacgccaac ataagagaaa cctatgccat tacccgattt
    29701 tcatgtttct gaacctttta ccctcggtat tgaactggaa atgcaggtgg ttaatccgcc
    29761 gggctatgac ttaagccagg actcttcaat gctgattgac gcggttaaaa ataagatcac
    29821 ggccggagag gtaaagcacg atatcaccga aagtatgctg gagctggcga cggatgtttg
    29881 ccgtgatatc aaccaggctg ccgggcaatt ttcagcgatg cagaaagtcg tattgcaggc
    29941 agccgcagac catcatctgg aaatttgcgg cggtggcaca cacccgtttc agaaatggca
    30001 gcgtcaggag gtatgcgata acgagcgcta tcaacgaacg ctggaaaact ttggctatct
    30061 catccagcag gcgaccgttt ttggtcagca tgtccatgtt ggctgcgcca gtggcgatga
    30121 cgccatttat ttgctgcacg gcttgtcacg gtttgtgccg cactttatcg ccctttccgc
    30181 cgcgtcgcca tatatgcaag gaacggatac gcgttttgcc tcctcacgac cgaatatttt
    30241 ttccgccttt cccgataatg gcccgatgcc gtgggtcagt aactggcaac aatttgaagc
    30301 cctgtttcgc tgtttgagtt acaccacgat gatcgacagc attaaagatc tgcactggga
    30361 tattcgcccc agtcctcatt ttggcacggt ggaggttcgg gtgatggata ccccgttaac
    30421 ccttagccac gcggtaaata tggcgggatt aattcaggct accgcccact ggttactgac
    30481 agaacgcccg ttcaaacatc aggagaaaga ttacctgctg tataaattca accgtttcca
    30541 ggcctgtcgc tatgggctgg aaggcgtcat taccgatccg cacactggcg atcgtcgacc
    30601 gctaacggaa gacaccttgc gattgctgga aaaaatcgcc ccttccgcac ataaaattgg
    30661 tgcatcgagc gcgattgagg ccctgcatcg ccaggtcgtc agcggtctga atgaagcgca
    30721 gctgatgcgc gatttcgtcg ccgatggtgg ctcgctgatt gggctggtga aaaagcattg
    30781 tgagatctgg gccgatgact aagtagggaa ttgccttaca gaagaatatc cctgacaatg
    30841 gtgttttaac ttacatttaa caatgttatg aaacaccctt tagaaacctt gactaccgca
    30901 gcaggcattt tgctgatggc tttcctctct tgcctgctgc tgcccgcccc cgcactgggg
    30961 ctgacgctgg cacaaaaact ggtgaccatg ttccatctga tggatcttag ctagctttac
    31021 actttattgt tttgtctgtg gtttttagtg ctgggcgcta ttgagtattt tgttctgcgc
    31081 tttatctggc gacgctggtt ctcgctggcg gattaaacgt cgattatcgc cgtacttcgc
    31141 ggcatacttt gcttattctc cttcgcctaa aggaacgttt atggataagc aatcactgca
    31201 cgaaacggcg aaacgcctgg cccttgagtt accctttgtc gagctttgct ggccttttgg
    31261 cccggagttc gatgttttta aaattggcgg caaaattttt atgctgtcgt cggagctgcg
    31321 cggcgtcccc tttatcaatc tgaagtccga tccacaaaaa tccctgttaa atcagcagat
    31381 atacccgagc attaagccag ggtatcacat gaataaaaag cactggattt cggtgtatcc
    31441 cggcgaggaa atctccgaag cgttacttcg cgatctgatc aacgattcgt ggaatctggt
    31501 ggttgatggt ctggctaaac gcgatcaaaa aagagtgcgt ccaggctaaa gcggaaatct
    31561 atagcgcatt tttctcgctt accatttctc gttgaacctt gtaatctgct ggcacgcaaa
    31621 attactttca catggagtct ttatggatat catttctgtc gccttaaagc gtcattccac
    31681 taaggcattt gatgccagta aaaaacttac cccggaacag gccgagcaga tcaaaactct
    31741 cctgcaatac agcccatcca gcaccaactc ccagccgtgg cattttattg ttgccagcac
    31801 ggaagaaggt aaagcgcgtg ttgccaaatc cgctgccggt aattatgtgt tcaacgaacg
    31861 taaaatactt gatgcctcgc acgtcgtggt gttctgtgca aaaaccgcga tggacgatgc
    31921 ctggctgaag ctggttgttg accaggaaga tgctgatggc cgctttgcca cgccggaagc
    31981 gaaagccgcg aacgataaag gtcgcaagtt cttcgctgat atgcaccgta aagatctgca
    32041 tgatgatgca gagtggatgg caaaacaggt ttatctcaac gtcggtaact tcctgctcgg
    32101 cgtggcggct ctgggtctgg acgcggtacc catcgaaggt tttgacgccg ccatcctcga
    32161 tgcagaattt ggtctgaaag agaaaggcta caccagtctg gtggttgttc cggtaggtca
    32221 tcacagcgtt gaagatttta acgctacgct gccgaaatct cgtctgccgc aaaacatcac
    32281 cttaaccgaa gtgtaattct ctcttgccgg gcatctgccc ggctatttcc cctcagattc
    32341 tcctgatttg cataaccctg tttcagccgt catcataggc tgctgttgta taaaggagac
    32401 gttatgcagg atttaatatc ccaggttgaa gatttagcgg gtattgagat cgatcacacc
    32461 acctcgatgg tgatgatttt cggtattatt tttctgaccg ccgtcgtggt gcatattatt
    32521 ttgcattggg tggtactgcg gaccttcgaa aaacgtgcca tcgccagttc acggctttgg
    32581 ttgcaaatca taacccagaa taaactcttc caccgtttag cttttaccct gcaggggatt
    32641 atcgtcaata ttcaggcggt attctggctg caaaaaggca ccgaagcggc agatattctg
    32701 actacctgcg cgcagttgtg gatcatgatg tatgcgctgc tttcagtctt ctcgttgctg
    32761 gatgttattt tgaatctggc gcagaaattc ccggcggcat ctcagttacc gctgaaaggg
    32821 atatttcagg ggattaaact gatcggcgcg atcctggtcg gcattttgat gatctcgctg
    32881 ctgattggtc agtcgccagc gattctgatc agcggtcttg gtgcaatggc tgccgtgctg
    32941 atgttggtat ttaaagatcc gattcttggt ctggtggccg gtattcagct ttccgcgaac
    33001 gatatgctga aactgggcga ctggctggag atgccgaaat acggcgcaga tggcgcggtg
    33061 atcgatattg ggttaaccac cgtcaaagtg cgtaactggg acaataccat taccactatt
    33121 cccacctggt ctctggtttc tgactccttt aaaaactgga gcgggatgtc agcatctggc
    33181 gggcgacgta ttaagcgcag tatcagtatt gatgtcacca gtattcgttt tcttgatgaa
    33241 gacgaaatgc aacgtctgaa taaagcgcat ttgttaaagc cttatttaac cagccgccat
    33301 caggaaatta atgagtggaa tcgccagcaa ggttctacgg agtcggtatt aaacctgcgc
    33361 cgaatgacca atattggaac ctttcgtgcc tatctgaacg aatatctgcg taaccatccg
    33421 cggattcgta aagatatgac cttaatggta cgccaactgg ctccaggtga taacggttta
    33481 ccgctcgaga tctacgcgtt tactaacacc gtggtgtggc tggaatatga aagcattcag
    33541 gctgatatat tcgatcacat atttgcgatt gtcgaagagt ttggtctgcg acttcatcag
    33601 tcgccaaccg gcaatgatat tcgctctctg gcgggtgcat ttaagcagta attaaaaaaa
    33661 ccgctctcat cgaatggatg agagcggaca tcgttttgtc ggatgtagcg tgaatgcctt
    33721 atttccgacg cagcgtttta aatgccacaa acaggaatac aatccacacc ggcagcagga
    33781 tcgctgacaa gcgcatatca tccatcgtgc acatcagcag caaaatcatg ccgaggaagg
    33841 caatgcagag atagtttccg aacggataga gcagcgcctt aaactgtgtt tcacgcccct
    33901 gacgtcgcat cgctgcacga aaacgcagat gcgccagaca gatcataatc cagttcaaca
    33961 gcagcgttgc taccaccagc gccatcagca gaccaaacgc tttttgcggc agcagatagt
    34021 tgattaacac caccagcgaa gtgatcgctc cggaaagcat cagcgagtta atcggcacac
    34081 cgcgacggct gacgcgagtc aaaaacttcg gcgcattacc ctgcacagaa aggccaaaca
    34141 gcatgcggct gttagagtaa accccgctgt tatacactga cagcgatgct accagaatga
    34201 cgaagttcag cgcagaagct accacgttgc tgtcgagatt atggaaaatc atcacaaacg
    34261 ggctactgtt ggatttcact tccacccacg gatagagcgc cagtaaaacc accagtgaac
    34321 cgatgtaaaa cagcaggatg cgatacacca cctgatttac cgcttttgga atgcttttat
    34381 ccggatcgcg cgcttcagcg gcagtaatcc caatcagctc cagaccgccg aaggagaaca
    34441 taattaccgc cagcgacaaa atcagcccat tccagccggt ggcgaagaaa ccaccgtagc
    34501 gccagaggtt gtcgatactg gctttctcgc cgccgtgacc agaaaacagc agccacaggc
    34561 caaagccgat cataccgatg attgccagca ctttaatcaa cgcaaaccag aactcggttt
    34621 cgccatataa gcgcacgttc accaggttaa cggcgttgat gataataaag aaggcggcag
    34681 cccaaatcca cgttggaaca tccgggaacc agtactgcat atagatgccc gcagcggtca
    34741 gctctgccat tcccaccagc acgaacatta cccagtagtt ccagccagag aggaagcccg
    34801 caaacggtcc ccagtattta taggcaaagt gggcaaatga accggatacc ggctcttcga
    34861 ccaccatctc gccaagctgg cgcataatca ggaaagcgat gatcccggcg acgccgtagc
    34921 ccagcaatac agccggaccc gccatctgaa tcgccggacc aatgccaaga aacagaccag
    34981 taccaattgc gccacccagc gcaatcagtt gaatatgacg gttatgtaat ccgcgatgaa
    35041 gcgtcggctc ttgattcgac gcagtatctt ccgatacggt tgacgcgttt ttcacgcctt
    35101 tcccctgtgt gtcttttttg ttgaggggca ccttttaaca tttagtgccc atcgtcgcaa
    35161 gacacaatcc acacggttaa acggggtatc ctgcttttat ttccgtaccc gatgtcggtg
    35221 cagccacatc agcttatacg ccgccgggat aataaacagc gacagcaaag gtgcggtgat
    35281 catgccgcca atcatcggtg cggcaatccg gctcatcacc tctgaaccag cccccgttcc
    35341 ccacagaatc ggcagcagac cggcgataat caccgccacc gtcatcgctt tcgggcgcac
    35401 gcgcagtacc gcgccgtgat acaacgcctc atccagcttc tgctcactga atgtttgcgg
    35461 attattcaac gacggctcgg cctctatggc gtgacgtaaa tacatcagca tcaccacgcc
    35521 aaattcggcg gcgaccccgg caagggcaat aaaaccggtg cccgttgcca cagaaagatg
    35581 aaagcccatc caccacagca accagatgcc gcccaccagc gcaaacggta cgctgctgat
    35641 aatcagcaac gcttcgccca cccgacggaa tgccagatac aacagcacga agatgatcat
    35701 cagtgtcatc ggcaccatga gtttcagctt atggttggcg cgctccagta gctcgaactg
    35761 cccggagaat gccacgctgg tgcccggttt taactgcact ttttcagcta tcgctttttg
    35821 caaatcgtga accaccgaca ccatgtcacg atcgcgggca tcgatataaa tccagctcgt
    35881 cgggcgcgca ttctcggttt tcagcatcga cggtccggta gagactttaa cgtcggccac
    35941 gtctgccagg gtgatttgct gcttcatcgg cgtcaggatc ggtagctggc gcagtgcctg
    36001 cgggctatcg cgccagcttt gcggataacg cagattaatg ggataacggg caatcccttc
    36061 caccgtttcg ccaaccatcg ccccgcccac cgcagaagtc acaaacaact gcacatccgc
    36121 caccgtcata ccgtaacgcg cagctttttc acggttaatc tcaacgttga tatagcgccc
    36181 accttccaga cgctcggcta gagcagaagc cacgcttggc accgttcgcg ctacttcttc
    36241 aatttgctca gccatcgcgt cgatatccgc cagcacagtg ccggaaactt taatgccgat
    36301 ggggctttta atgccggttg agagcatatc gatacggtta cgaattggcg gcacccacag
    36361 attcgccagc cccggcagcc gcacggtgtt atccagttcc tcaatgattt tgtccatcgt
    36421 catgccaggc cgccactgat cctgcggctt aagctggatg gtcgtttcaa ccatctcgag
    36481 cggagcagaa tcggtggcgg tttccgcttt cccggttttg ccaaataccc gcgccacttc
    36541 aggtacgctc ataattagct tgtcggtttt ttgcagcata ctcgccgcct ctgctgcgga
    36601 aatccccggc agcgtcgatg gcatatacaa caagtcgcct tcattgatct gcggtaaaaa
    36661 ttccccgcca actttattga gcggccagag aaccgtcagc accgaaagcg ctgccaccaa
    36721 cagcgtggtt ttcggccagt gcagtacttt cagcaataga ggatgataaa cacgaatcaa
    36781 aaagcgattg agcgggttac tgctttccgg cggaattttg ccacggatcc agtaccccat
    36841 caggataggg atcactacga tcgccagcag cgccgcgccc gccatcgcat aggttttagt
    36901 gaacgccaac gggccaaaca gacgcccttc ctgcccttcc agggtgaaga tcgggataaa
    36961 cgacaacgtg ataatcagca gactgataaa tagcgccgga cccacttcaa cagatgcatc
    37021 ggtgatcacc tgccagcgcg ttttattatc cagcgtggcg tcaggatgct ggtgctgcca
    37081 ctcttccagc cgtttatgtg cattttcgat catgacgata gcggcatcga ccatcgcccc
    37141 gacggcaatc gcaatgccgc ccaacgacat aatattggca ttcagtccct ggaagtgcat
    37201 gacaataaaa gcaatacaca accccagcgg caacgaaata atcgccacca gcgccgagcg
    37261 cacatgccag agaaacagcg cacagactac cgccaccacg ataaactctt ccagcaactt
    37321 gccgctgagg ttgtcgatgg cgcggtcaat gagctggctg cgatcgtatg ttgtaactat
    37381 ctccacgcct tccggcagac tacttttcag cgtttccagt ttgtccttca cggaggcgat
    37441 cacttctcgg gcgtttttgc cggagcgcag gatcaccacc ccgcccgcca cttcgccttc
    37501 gccattcagt tcggcaatgc cccggcgcat ctccgggcca atctggacct tcgcaacatc
    37561 acgcagataa acgggcacgc cattttcact ggcttttaaa acgatgtgat taaagtcgtc
    37621 gagcgtttgc agatagccgc tggcgcgcac catatattcc gcttccgcca gttcgatcga
    37681 cgaaccgccc gcttcctggt ttgaagcatc cagcgcgctt tttacttcgg ctagactgat
    37741 gccatattgc gccaggcgct ggggatcgat aaccacctga tactctttca ccacgccgcc
    37801 caccgacgcc acttccgcaa cgtcagggat ggtttttagc tcatatttga gaaaccagtc
    37861 ctgcaatgag cgtaaatcgg ccagatcgtg cttaccgctg cgatccacca gtgcatattc
    37921 atagatccag ccaacacccg tggcatctgg tcccagctcg gcgctgactc ccgcaggcag
    37981 cttaccctgt acctggttga ggtactccag cacccgcgaa cgcgcccagt acggatcggt
    38041 accatcttcg aaaatgacat acacataaga gtctccaaac tgcgagaaac cgcgcacagt
    38101 cttcgcgcca ggcaccgaca acatggtggt ggttagcgga taagtcacct gattttcaac
    38161 gatttgcggt gcctgaccgg gatagctggt tttaataatc acctgcacat cggagagatc
    38221 cggcagcgca tccactggcg tgttaatgat ggtccaggtg ccccagatgc tcagaaacaa
    38281 cgcgcccatc agcaccaaaa aacggttcgc caccgagcga cgaataatcc attcaatcat
    38341 tggttattcc ctcaatgcgc attggtagca ctttcagagc gcatccgctc cagtgcgcca
    38401 gaaatattgg cttcggaatc aatcaggaac aggccgctgg aaaccacctt ttcaccttcc
    38461 gccagaccag agtgtaacgc ggtgacgcct tgcgatgcct ggaaaacggc aacgcgtttc
    38521 ggtacaaagc gtccgtcggc atcaacggta atcacccgct gttcgttgcc ggtatcaatc
    38581 agcgcctgtg acggaatgag cagcatcggt tcgctggcgg tgttgagttg caaccaggcg
    38641 ttcatgcccg gttttagcgc ctcgtcggcg ttgtcgactt ccagacgcag ctgcagcgtg
    38701 cgggtcgcgg catccacgcc aggtagcagc gtccatttgc ggatggtgag tgttttatcc
    38761 ggtcgcgccg gaacggtgag cgtaaactgc gaggcatctt tcaccagcca ggcgatagac
    38821 tccgggatcg cagcagtgac ccacaccggg tccataccct gaattttcgc taccacgtta
    38881 tctttggcga tattcattcc cgcgcgcaga tcaaacgcgg tgatcacgcc atcaatgggt
    38941 gctttgagcg taaagcgagt ctggattttt tgcgtggcga tcaggcggcg aatatccgcc
    39001 tccggcattc ccgccagtcg cagccgctca agaatgcctt cagtctgggt cgccgtaccg
    39061 ccggtttcgc gcagcagtaa atactcactc tgcgcttcca cccagtcagg aatggtcagg
    39121 tcgagaagcg gtgtgccctt ttgtacttta tcgcccacgg taagcggata caccttgtcg
    39181 ataaacccgg cagcgcgagc ctgcacaatg gcatactgat actcgttgta actgacattc
    39241 gccgggaaac tctgggcaaa agtcagcggt ccgcgcgtga cggtagccgt tttcaccccc
    39301 agattctgcg tctgagtcgg gtcaatgcgc acaccagacg cagaactctc ttcatcagca
    39361 tatttcggca ccagatccat atccataaac ggcgatttac ctggtttatc gaaccgcgta
    39421 ttggggtaca tcgggtcgta ccagaataag attttacgtt ctgcggtcga cgttttttct
    39481 gcgggtggtt cagcctttgc aaaccaggta aaacctgccg cagaaataat accgcccgcg
    39541 atcatgctgc cgataataag cgcgattttt ttcatctcat taaacctggg ttactggctg
    39601 actttaatat cctgtaataa agaaaggttg ccctgctgga caaaattaaa cgccactttg
    39661 tcgccggttt taatttcact cattttcgtc tgcggggtga tggtaaagcg catagtcatc
    39721 tccggccagt tcacggcagc aatcggatcg tgatggatgg tgattttttt gctttccaga
    39781 tcaataccct ttaccacgcc agtggcgcta ataacctgcg gttgtacttc gctcatggtt
    39841 tcatgatgat gttcgttagc ctgggcatta aagccaataa cggtaaacag actgaacatt
    39901 gcgacttgca gtgctttttt catttattct ctcctggagt taaaaattat tgctgccaac
    39961 cgccccctaa ggcggtatac aaagaaattt cgttaacctg acgggcataa ttcaggtcga
    40021 gtaaagtttg tcgggttgca aataaagaac gctcggcatc cagcacttcc agataactta
    40081 ccgcgccgtg ctgatataat gcccgcgccc gttgcaaagt aatttgcagc gacgccagat
    40141 aacgctgctg ggcgctgatt tgatcgttca ggctttgacg taatgccagc gcatctgcca
    40201 cttctttaaa ggcgttctgg attttctgtt cataattcac taccgactgc tgctggcgaa
    40261 tttcggcgat atccagattg gcctggttgc gtccggcatt aaaaatgggg atctcaattt
    40321 taggaataaa attccacatc ccgctgctag cgttaaataa tgaagacaga tcgctgctgg
    40381 cggttgatat tccgctggtc aggctgatag acgggaaaaa tgccgcacgc gcagcaccaa
    40441 tattagcatt agccgccatt aacgcgtgtt cagcttccat aatatccggg cgctgcaata
    40501 agatttgcga cgacaagccc gccggtaatt taacgctttg caggctgccg ctgtttactg
    40561 tctgcgcttg cggcagtttg ccgtagcttc ccaataacag ttgcaatgca ttattcgcct
    40621 gcgccagttc cccctgacgt ttagcgatgt cactgcgggt actttctatc accccgcgag
    40681 cctgttccag cgccagaaca ttgctgctac cggtcaacag ttgtttttcg acaaacgcat
    40741 atgactgctg ataattacgc agcgtttctt cggctatttg caattgcgca tacgccagtt
    40801 gctgattgaa atagctttgc gcgacattag aaaccagcag aatatgcacc gcgcgctgag
    40861 cttcctcagt ggctaaataa ttttgtcgct cggcttcgct catgttcttt aagcgaccga
    40921 aaaaatcgag gtcaaagctg gcgttaaggc cggtcgaaaa ctcccgtgtc gtggcggtat
    40981 tccctttaag attaccgctc cagctgccgc tgccctcgcc attgagctgt gggtagcggt
    41041 cggcatcggt cagacgatat tgcgcccgtg cctcctgcac tttcagcgtc gccatgcgca
    41101 aatcccggtt attcaccagc gcctcgctaa tcagcgtctt cacctgatta tcaacaaaaa
    41161 aggtgcgcca gcccacgttc tggtagttat ctgctgcgtt aaccaagccg ttctggctga
    41221 gtgagaactg ctgcggcacg ggcattgccg tacgctgata atccggtgcc agtgaacaac
    41281 cggttagcgc aagggccaca caaaatggca gaagtttaca aggagacata ggctcataat
    41341 ttctggtgat tttataccgc caactttact cgccaggctc tgattttccg gtgacaggaa
    41401 aatgacaaaa ttgtcatttt gccaataagc gattgccatc tgatcccgct actctagaat
    41461 tgcccgggca acatgcggag gaaaaatgaa actgttgatt gtcgaagatg aaaagaaaac
    41521 cggagaatac ttgaccaaag ggttaaccga agccggtttt gtggtcgatt tggccgacaa
    41581 cgggctgaat ggctaccatc tggcgatgac cggtgattat gacctgataa tcctcgatat
    41641 tatgctgccg gacgtgaacg gctgggatat cgtgcgcatg ttgcgctccg ccaataaagg
    41701 gatgccgatt ctgttgctta ccgcgcttgg caccattgaa catcgcgtca aggggctgga
    41761 gttgggggcc gatgactatc tggtgaagcc gttcgctttt gctgaactgc tggcgcgggt
    41821 gcgcactctc ctgcggcgcg gggcggcggt gattatcgaa agtcagtttc aggttgccga
    41881 tctgatggtc gatctcgtca gccgcaaagt cacccgcagc ggcacgcgca tcactctgac
    41941 cagtaaagag tttactctgc tggagttttt tctccgccat cagggcgaag tgctgccccg
    42001 ctcgcttatc gcctcgcagg tgtgggacat gaatttcgac agcgacacta acgccattga
    42061 tgtggcggtg aagcggctac gcggcaaaat cgacaacgac tttgagccga agctgattca
    42121 gaccgtgcgc ggcgtgggct atatgcttga ggtgccggat ggtcagtaag ccatttcagc
    42181 gcccgttttc gctggcaacc cgtctgacct tttttatcag cctggccacc atcgcggcgt
    42241 ttttcgcctt tgcatggatc atgatccact cagtgaaagt gcattttgcc gagcaggata
    42301 ttaatgattt aaaagaaatt agcgccaccc ttgaacgggt acttaatcat cctgacgaga
    42361 cgcaagcccg acgcttaatg acgctggaag atatcgtcag tggttattcc aacgtgttga
    42421 tttccctggc agatagccac ggcaaaacgg tgtatcactc ccccggtgcg ccggatatcc
    42481 gcgagtttac gcgtgacgcc atacccgata aagacgctca gggtggcgag gtgtatctcc
    42541 tttccggccc gacgattatg atgccaggcc acggtcacgg gcatatggaa cacagcaact
    42601 ggcggatgat taacttgccg gttggcccgt tggtggacgg caaaccgatt tatacgctct
    42661 acatcgcgct ttcgatcgat tttcatcttc attacataaa tgatttgatg aataaactta
    42721 ttatgaccgc atctgtcatc agcatcctga tcgtctttat cgtgctattg gcggtacata
    42781 aaggtcacgc gccgatccgc agcgtcagcc gtcaaatcca gaatatcacc tcgaaagatc
    42841 tcgacgttcg ccttgacccg cagaccgagc ccattgagct ggaacagctg gtactgtcgt
    42901 tcaaccatat gatcgagcgt attgaggatg tctttacccg ccagtccaat ttctcagcgg
    42961 atatcgctca cgaaattcgc acgccgatta ccaatctcat cacgcaaacg gaaatcgccc
    43021 tcagccagtc tcgcagccag aaggagctgg aagatgtgct ctactctaat ctcgaagagc
    43081 tgacgcgaat ggcgaaaatg gtcagcgata tgctgtttct cgctcaggcc gataacaacc
    43141 agctaatccc cgaaaagaaa atgctcaacc tggcggatga agtcggcaaa gtgttcgatt
    43201 ttttcgaggc gttagcggaa gatcgcggcg tggagttgcg atttgttggc gacaaatgtc
    43261 aggttgcggg cgatccgctg atgctgcgtc gggcgttaag caacctgctt tctaatgccc
    43321 tgcgttatac gccaaccaga gagacaattg tagtgcgctg tcagacggtc gatcaccagg
    43381 tgcaagttac cgtcgaaaac cccggtacgc ccattgcgcc cgagcactta ccgcgattgt
    43441 ttgaccgttt ctaccgcgtt gacccttccc gccagcgaaa aggcgaaggt agcggcattg
    43501 ggctggcgat agtgaaatcg attgttgtcg cgcataaagg cacggttgca gtaacgtcag
    43561 atgcgcgggg gacaaggttt gtgattatgc tgccggagag agagtgatta ttaagttcaa
    43621 attcagggcg tggtgcatca cctcctggta ttgttgatca acaaattaga gaaaaactta
    43681 ttaaacatat gaaatatcga aggcttttat ttttcaaagt ttcacgagat gtttgtatat
    43741 gaaacttagt taaatctgtg tttcttccaa ttgtagatca caatgccctt tctaaatttt
    43801 catcgttata aacaagccat ctatcatata aagcaatgaa taatttaatc gcgattaatc
    43861 atcattaatt gttatattat tcactcaatc tatttgtcaa gtgaatttga taaattccta
    43921 atttaaaata tccactattg agccagtatt taacaacgtt aagatgtgca atgtgcagat
    43981 catgttatca gggtggagcg gtagcatata tttcttatgc aatcattcat tttaataatg
    44041 ttaattggat tatttattgc tgtgaataaa aatatattat gaatattggt ttaactttgc
    44101 gtttttgtta ctcgttgtga tatgcgtacc tgtcctgaat aacgccaaga aacaagaggt
    44161 tgagcttgtg attatttttt tgactgaaca gacacccgtt tctgtaaagt aatatttaaa
    44221 caaaattctc acataaccat agcattttct gttccttccc taataacgtc tgaagtcgtc
    44281 ccgcttatca cattatccgc agttgtacgg tacggatcat tattcatttt actggaggtt
    44341 gctatgtcaa ccggattacg tttcacactg gaagtggacg gcctgccgcc ggacgctttt
    44401 gcggtggttt cctttcatct gaaccagtca ctctcttcgc tcttttccct cgatctctcc
    44461 ctggtcagcc agcagtttct ctcccttgaa tttgcgcagg tgctggacaa aatggcctac
    44521 ctgacgatat ggcagggcga tgaagtacag cgccgggtga aaggcgtggt gacctggttt
    44581 gaactcgggg agaacgacaa aaaccagatg ctgtacagca tgaaggtgca cccgccgctg
    44641 tggcgtgccg gtctgcgcca gaacttccgt atcttccaga acgaggacat caaaagcatc
    44701 ctcggcacga tgttgcagga aaacggggtg accgaatgga gtccgctgtt cagcgagccg
    44761 catccttccc gtgagttttg tgtccagtac ggtgagactg attacgattt cctgtgccgg
    44821 atggcggcgg aggaaggcat cttcttttat gaggagcatg cttacaaaag taccgaccag
    44881 agcctggtgc tgtgcgacac agtccgccat ctgcccgaat cttttgaaat cccatggaac
    44941 ccgaacaccc gtaccgaggt gagcaccctc tgcatcagcc agttccgcta cagcgcacaa
    45001 atccgccctt cttccgtggt gaccaaagac tacaccttta aacgccccgg ctgggccgga
    45061 cgttttgaac aggaaggcca gcaccaggac taccagcgca cgcagtatga agtgtatgac
    45121 taccccggac gtttcaaggg tgctcacggg cagaactttg cccgctggca gatggacggc
    45181 tggcgaaaca atgcagaaac cgcgcgggga atgagtcgct cgccggagat atggccggga
    45241 cgacgaattg tgctgacggg gcatccgcag gcgaacctga accgggaatg gcaggtggtg
    45301 gcaagtgaac tgcacggcga acagccacag gcggtgccag gacggcaggg agcggggacg
    45361 gcgctggaga accattttgc ggtgatcccg gcagacagaa catggcgacc acagccgttg
    45421 ctgaaaccgc tggtcgacgg cccgcagagc gctgtcgtga caggaccggc aggcgaggaa
    45481 atcttctgcg acgaacatgg tcgcgtgcgg gtgaagttca actgggaccg ttataacccg
    45541 gcagaccaag acagttcgtg ctggatccgt gtggcacagg cgtgggcagg caccggtttt
    45601 ggccacctgg cgataccgcg tgtgggtcag gaagtgattg tagacttcct caacggcgat
    45661 ccggaccagc cgatcattat ggggcgcacc taccaccagg aaaaccgcac ccccggcagc
    45721 ctgccgggaa caaagacgca gatgaccatc cgctccaaaa cgtacatggg cagcggattt
    45781 aatgagctga agtttgatga tgcgacaggg agagaacagg tctacatcca cgcgcagaag
    45841 aacatggata ccgaagtgct caacgaccgt accaccaccg taaaacacga tcaccgcgaa
    45901 accgtaaaaa atgaccagac ggtcacgatc caggaaggta accgccttct tacggtggaa
    45961 aaaggccaca agatcaccgg agtactgaaa gggtctttat ctgaggatgt ctttcaggac
    46021 agaggcacga ttgccggttc ggtgcatgtt gacgctgtaa acaatggtgg cgaaggcgac
    46081 ggtatacagg cttatacggc gattaaggaa attttgctgg ccgtggagga aagcaaaatt
    46141 gcgctgacgc cggatagcat tcagctacag gtcggggaat cgacggtaat caggctgtcg
    46201 aaggatggca tcaccatcgt gggcggttct gttttcatca actgattcac tcagattatc
    46261 agggagtatt ttatgcagtt cacttttaat gaaggccaca ttcaactgcc gtctcaatgg
    46321 caggatcagt caatgcaggt cctggtgtcc acagataaca gtggcatcaa cctggtcatt
    46381 acccgcgaag ccgttccaca gggaaccctg acaccagagc tgtaccagga aacgctggcg
    46441 ctgtaccagg gcaaacttga tgggtacacc gaacatgcct gccgggaaat cactctggca
    46501 gaggccccgg cctggctgct ggattattcc tggaacggtc cggaagatga ggggaatcag
    46561 gggcggatta gccagatagc cgtattccag cggcgtggag atacgctgct gaccttcact
    46621 ttcagcacct ccttatccct gaagaacagt caaaagacga tgctgctgga ggtgataaag
    46681 agcttcaccc cgttgccacc ggagaatgac atccaaaagg accaggcggg ccacagggag
    46741 cgaccgcagg cggtacgctg gcaatgcgaa tgctgtcaca gcaggcgatg gtcgccagcc
    46801 agatgaaacg ggcagccaac gacaaagcca ttgcacagat gttggcatca aaaaagtctg
    46861 gcccccccgc cgccaggctg ggcgatgaaa ttcagcacaa gagttttttg ggggcgctgg
    46921 caggggccgt actgggggcg atagtgacca tcgcagaagg ttgcctgatt atggccgcct
    46981 gtgccaccgg cccttatgcg ctggttctgg tacctgcgct gatgtatgcc agctataagg
    47041 cgagtgatta tgtggaggag aaacagaacc agcttgaatc atggataaac agcttttgtg
    47101 acacggacgg cgccatcaat accggttctg aaaacgtaaa aattaacggc gagctggccg
    47161 cacgtgcagc ggtcaccctt ccccctcctc ccccacctgg agcaatacct gaagtcccac
    47221 agggggaacc ctcatggggt gatattgcca ctgacctgct tgaatcggca gcggaaaaag
    47281 cagtaccact ggcgaaggcc tgggggaacg ctgttatcac cctgacggac agcaatgccg
    47341 gttttatgga tcgcgtcagc gccggcgcat cgcttctgtt tcccgccggt ccggtattaa
    47401 tggagtttgc caccatggtg ggcgggcgtg gcgaaatcaa aaaagatgtg gatttcccgg
    47461 aagccggtga ggacacggcg ctctgcgaca aggagaacaa accaccgagg atagcccagg
    47521 gcagcagcaa cgtctttatc aacaatcagc ctgccgcgcg caagggcgac aaactggagt
    47581 gcagtgcggc gatcgtggga ggttcgccgg acgtctttat tggcggtgag caggtcacat
    47641 atctggatat ccagccggag ttcccgccgt ggcagagaat gatcctgggg ggaataacga
    47701 tagccagcta tcttctgccg ccagcgggac tactgggaaa actgaagaat ctggcgagac
    47761 tcggcaaact gggaaactgc tggggaaaag cgggaagctg ctgggcgcaa agctcggcgc
    47821 gttgctgggg aaaacaggta agtcgttaaa aagtattgcc aataaagtca tcagatgggt
    47881 aacagatcct gtcgatccgg taaccggcgc atactgcgac gaacgtaccg acttcaccct
    47941 gggccagacc ctccccctct ccttcacccg tttccacagt tctgtactgc cgctgcatgg
    48001 cctgaccggc gtgggctgga gcgactcctg gagcgaatac gcctgggtgc gtgaacaggg
    48061 aaaccgggtg gatatcatca gccagggagc cacgctgaga tttgccttcg acggtgacag
    48121 tgatacggcg gttaacccgt atcacgccca gtacattctg cgccgccgcg atgattatct
    48181 ggagctgttc gacagggatg cactgagcag ccgcttcttt tatgacgcct ttccgggaat
    48241 gcgtctgcgc cacccggtga ctgacgatac cagcgatgac cgcctggcac acagccccaa
    48301 tgaccggatg tacatgctgg gcgggatgag cgacaccgcc agcaaccgca tcacgtttga
    48361 gcgcgacagc cagtaccgga tcacgggtgt cagtcacacc gacgggatcc ggcttaaact
    48421 gacgtaccac gccagcggct acctgaaagc cattcaccgc acggataacg acatacagac
    48481 gctggcgacc tacgaacagg atgcgcgggg gcggctgaca gaagcggatg cgcggctgga
    48541 ctaccacctg ttttatgagt acgacgctgc ggaccggatc atccgctggt ccgataacga
    48601 ccagacgtgg agccgtttca cctacgatga acagggccgg tgcgtgaatg tcaccggggc
    48661 ggagggctat tacaacgcca cgctggacta tggtgacggc tgcaccaccg tgacggacgg
    48721 caagggcatt caccgttatt actatgatcc tgacggcaat attctgcggg aagaagcgcc
    48781 ggacggcagc accaccatgt atgaatggga tgaattccat cacctgctgg cccgccactc
    48841 ccctgccggg cgggtggaga aatttgaata caacgccgca cacggtcagt taagccgtta
    48901 cacggcggca gacggcgcgg agtggcagta ccgctatgat gagcgcggcc tgctcagcaa
    48961 catcaccgac cctgccgggc agacgtggac acagcagtgt gatgaacgcg gcctgccggt
    49021 aagtctggtg tcgccacagg gcgaagagac ccggctggca tacaccgctc aggggctgct
    49081 gtcgggaata ttccgccagg atgaacggcg tctgggcata gagtacgacc accacaaccg
    49141 gccggaaaca ctcaccgacg tgatgggccg tgaacaccac accgaataca gcggtcacga
    49201 cctgccagtg aagatgcgcg gccccggcgg tcagtcagtg cggttacagt ggcagcagca
    49261 ccataaactg agcggcattg aacgtgctgg aaccggcgcg gaaggatttc gctatgaccg
    49321 ccacggcaac ctgctggcct ggacggacgg caacggtgtt gtctggacga tggaatacgg
    49381 tccgttcgat tttccggtgg cgcgaacgga cggtgaaggc caccgctggc agtaccgcta
    49441 cgataaagac acgctgcaac tgacagaagt catcaacccg cagggtgagt catatcttta
    49501 tgttctggat aactgcggcc gggtgacgga agagcgtgac tggggcggcg tggtctggcg
    49561 ttaccgctat gacgccgatg gcctctgtac cgccagggtt aacggcctgg aggaaaccat
    49621 cctctacagc cgggacgccg caggccgcct ggcagaaatc atctcgccgg aaggcaaaac
    49681 gcagtatgcc tatgacaaat ccggcaggct gacgggtatc ttcagcccgg acggcacatc
    49741 acagcgcacc ggctatgacg aacgcgggcg ggtgaatgtc accactcagg gccgacgggc
    49801 cattgaatac aactaccccg atgaacacac cgtcatccgc tgtatcctgc caccggaaga
    49861 tgaacgcgac agacaccccg gtgaatccct gctgaaaacc acgtaccgct acaacgccgc
    49921 cggagaactg acagaggtca ttctgccggg ggatgagacg ctgacgttca gccgtgatga
    49981 ggcgggacgt gaagtgctcc ggcacagtaa ccggggtttt gcctgtgaac agggctggaa
    50041 tgcagccggt cagcctgtca gtcagcgcgc cggatttttt ccggcagaag ccacatggga
    50101 cgggctggtt ccctcactgg tacgggagta ccgttacgac agcgcgggta acgtgtcagg
    50161 cgtcaccagc cgggaagatt acggacggga aacacggcgg gagtatcggc tggaccggaa
    50221 cggccaggtc acggcggtga cagcctcagg caccgggctg ggctatggcg aaggcgatga
    50281 gtcctatggc tatgacagct gcggctacct gaaggcgcag tctgcgggca ggcaccggat
    50341 aagtgaagag actgaccagt atgccggagg ccaccggctg aaacaggccg gaaacacgca
    50401 gtatgactat gacgccgcag gccggatggt cagccgcacc agacaccgtg acggctaccg
    50461 accggaaaca gagcggttcc ggtgggacag ccgggaccag ctgaccgggt attgcagcgc
    50521 acagggtgag ctgtgggaat accgccacga cgccagcggc agacgaacag agaaacgctg
    50581 cgaccggaag aaaatccgct tcacgtatct gtgggacggc gacagtattg cggaaatccg
    50641 ggaataccgc gatgataaac tgtacagcgt aaggcacctg gtgtttaacg gctttgagct
    50701 gataagccag cagttcagcc gggtacggca ggcgcatccg tcggtggccc cgcagtgggt
    50761 gacgcggacg aatcatgcgg tgagcgacct gacgggccgc ccgctgatgc tctttaacag
    50821 tgaaggaaag acggtctggc gaccggggca gaccagcctg tgggggctgg cactcagcct
    50881 gcccgcagac accggctacc cggacccgcg cggggaactg gacccggaag ccgaccccgg
    50941 cctgctgtat gcaggacagt ggcaggatgc ggaatccggg ctgtgctata atcggttccg
    51001 gtattacgag ccggaaaccg gaatgtacct ggtgagtgat ccgctggggt tgcagggcgg
    51061 ggagcagact taccggtatg tgccgaatcc ttgtgggtgg gttgatccgc tgggattggc
    51121 tgcaagttct aaaatcagca gtttgatgga ctatattggc gatggtcgtc gtgttagtgg
    51181 gcatacgggt ttcctggatg gggttcgttt atcacgtagt caaataa
//
LOCUS       DAKISA010000018        49834 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.018, whole genome shotgun sequence.
ACCESSION   DAKISA010000018 DAKISA010000000
VERSION     DAKISA010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49834
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.018"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            194..253
                     /gene="ibsC"
                     /locus_tag="OVW45_001088"
     CDS             194..253
                     /gene="ibsC"
                     /locus_tag="OVW45_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCV5525595.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            585..644
                     /locus_tag="OVW45_001089"
     CDS             585..644
                     /locus_tag="OVW45_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCV5525596.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(745..1347)
                     /locus_tag="OVW45_001090"
     CDS             complement(745..1347)
                     /locus_tag="OVW45_001090"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCV5525597.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(1368..1551)
                     /gene="ssrS"
                     /locus_tag="OVW45_001091"
     ncRNA           complement(1368..1551)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OVW45_001091"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(1593..1922)
                     /gene="zapA"
                     /locus_tag="OVW45_001092"
     CDS             complement(1593..1922)
                     /gene="zapA"
                     /locus_tag="OVW45_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCV5525598.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            2090..2668
                     /gene="ygfB"
                     /locus_tag="OVW45_001093"
     CDS             2090..2668
                     /gene="ygfB"
                     /locus_tag="OVW45_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCV5525599.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            2694..4019
                     /gene="pepP"
                     /locus_tag="OVW45_001094"
     CDS             2694..4019
                     /gene="pepP"
                     /locus_tag="OVW45_001094"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCV5525600.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNESEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            4016..5194
                     /gene="ubiH"
                     /locus_tag="OVW45_001095"
                     /gene_synonym="visB"
     CDS             4016..5194
                     /gene="ubiH"
                     /locus_tag="OVW45_001095"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCV5525601.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            5217..6419
                     /gene="ubiI"
                     /locus_tag="OVW45_001096"
                     /gene_synonym="visC"
     CDS             5217..6419
                     /gene="ubiI"
                     /locus_tag="OVW45_001096"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCV5525602.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(6493..6576)
                     /locus_tag="OVW45_001097"
     CDS             complement(6493..6576)
                     /locus_tag="OVW45_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525603.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            6866..7960
                     /gene="gcvT"
                     /locus_tag="OVW45_001098"
     CDS             6866..7960
                     /gene="gcvT"
                     /locus_tag="OVW45_001098"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCV5525604.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVLEGI
                     GETAIVQIRNREMSVKVTKPVFVRNGKAVA"
     gene            7984..8373
                     /gene="gcvH"
                     /locus_tag="OVW45_001099"
     CDS             7984..8373
                     /gene="gcvH"
                     /locus_tag="OVW45_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCV5525605.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            8492..11365
                     /gene="gcvP"
                     /locus_tag="OVW45_001100"
     CDS             8492..11365
                     /gene="gcvP"
                     /locus_tag="OVW45_001100"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCV5525606.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAAVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            11631..12374
                     /locus_tag="OVW45_001101"
     CDS             11631..12374
                     /locus_tag="OVW45_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCV5525607.1"
                     /translation="MAIALVTDGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVN
                     LITQVGGKAFVLQADISDENQVIAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNMTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGVRVNCVRPGFIFTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(12431..13864)
                     /gene="bglA"
                     /locus_tag="OVW45_001102"
     CDS             complement(12431..13864)
                     /gene="bglA"
                     /locus_tag="OVW45_001102"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCV5525608.1"
                     /translation="MKKLTLQKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVITSNGEKL"
     gene            13909..14220
                     /locus_tag="OVW45_001103"
     CDS             13909..14220
                     /locus_tag="OVW45_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCV5525609.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            14384..15043
                     /locus_tag="OVW45_001104"
     CDS             14384..15043
                     /locus_tag="OVW45_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCV5525610.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(15239..16219)
                     /gene="ygfZ"
                     /locus_tag="OVW45_001105"
     CDS             complement(15239..16219)
                     /gene="ygfZ"
                     /locus_tag="OVW45_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCV5525611.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDEHVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEYPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            16462..16728
                     /gene="sdhE"
                     /locus_tag="OVW45_001106"
     CDS             16462..16728
                     /gene="sdhE"
                     /locus_tag="OVW45_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCV5525612.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            16709..17116
                     /locus_tag="OVW45_001107"
     CDS             16709..17116
                     /locus_tag="OVW45_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin CptA"
                     /protein_id="HCV5525613.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(17156..17677)
                     /gene="fldB"
                     /locus_tag="OVW45_001108"
     CDS             complement(17156..17677)
                     /gene="fldB"
                     /locus_tag="OVW45_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCV5525614.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            17789..18685
                     /gene="xerD"
                     /locus_tag="OVW45_001109"
     CDS             17789..18685
                     /gene="xerD"
                     /locus_tag="OVW45_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCV5525615.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            18710..19420
                     /gene="dsbC"
                     /locus_tag="OVW45_001110"
     CDS             18710..19420
                     /gene="dsbC"
                     /locus_tag="OVW45_001110"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCV5525616.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            19426..21159
                     /gene="recJ"
                     /locus_tag="OVW45_001111"
     CDS             19426..21159
                     /gene="recJ"
                     /locus_tag="OVW45_001111"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCV5525617.1"
                     /translation="MKQQIQLRRRAVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEADKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            21250..22348
                     /gene="prfB"
                     /locus_tag="OVW45_001112"
     CDS             join(21250..21324,21326..22348)
                     /gene="prfB"
                     /locus_tag="OVW45_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCV5525618.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            22358..23875
                     /gene="lysS"
                     /locus_tag="OVW45_001113"
     CDS             22358..23875
                     /gene="lysS"
                     /locus_tag="OVW45_001113"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCV5525619.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(23918..24466)
                     /gene="idi"
                     /locus_tag="OVW45_001114"
     CDS             complement(23918..24466)
                     /gene="idi"
                     /locus_tag="OVW45_001114"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCV5525620.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            24521..24592
                     /locus_tag="OVW45_001115"
     CDS             24521..24592
                     /locus_tag="OVW45_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525621.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(24589..24714)
                     /locus_tag="OVW45_001116"
     CDS             complement(24589..24714)
                     /locus_tag="OVW45_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525622.1"
                     /translation="MNFLMRVIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(24716..26164)
                     /gene="uacT"
                     /locus_tag="OVW45_001117"
     CDS             complement(24716..26164)
                     /gene="uacT"
                     /locus_tag="OVW45_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCV5525623.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFLHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            26600..28519
                     /gene="ygfT"
                     /locus_tag="OVW45_001118"
     CDS             26600..28519
                     /gene="ygfT"
                     /locus_tag="OVW45_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HCV5525624.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            28519..29007
                     /locus_tag="OVW45_001119"
     CDS             28519..29007
                     /locus_tag="OVW45_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCV5525625.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(29076..29426)
                     /gene="ghxQ"
                     /locus_tag="OVW45_001120"
                     /gene_synonym="ygfQ"
                     /pseudo
     CDS             complement(29076..29426)
                     /gene="ghxQ"
                     /locus_tag="OVW45_001120"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
     gene            complement(29462..30778)
                     /gene="guaD"
                     /locus_tag="OVW45_001121"
     CDS             complement(29462..30778)
                     /gene="guaD"
                     /locus_tag="OVW45_001121"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HCV5525626.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(30796..32196)
                     /gene="xanQ"
                     /locus_tag="OVW45_001122"
     CDS             complement(30796..32196)
                     /gene="xanQ"
                     /locus_tag="OVW45_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HCV5525627.1"
                     /translation="MSDINNAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGRLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(32361..35231)
                     /locus_tag="OVW45_001123"
     CDS             complement(32361..35231)
                     /locus_tag="OVW45_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HCV5525628.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLDVIGKHYPKTDAAKMVQAKPCYVEDCVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGIGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(35228..36007)
                     /locus_tag="OVW45_001124"
     CDS             complement(35228..36007)
                     /locus_tag="OVW45_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HCV5525629.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(36058..37386)
                     /gene="ssnA"
                     /locus_tag="OVW45_001125"
     CDS             complement(36058..37386)
                     /gene="ssnA"
                     /locus_tag="OVW45_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HCV5525630.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSELSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARLIDEAKKATSEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(37389..40487)
                     /gene="ygfK"
                     /locus_tag="OVW45_001126"
     CDS             complement(37389..40487)
                     /gene="ygfK"
                     /locus_tag="OVW45_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HCV5525631.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMLQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRENLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMVLAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCNKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYEDKITVFSLAQDFDNSS
                     TPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(40809..41387)
                     /gene="mocA"
                     /locus_tag="OVW45_001127"
     CDS             complement(40809..41387)
                     /gene="mocA"
                     /locus_tag="OVW45_001127"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HCV5525632.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMFPWEQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            41490..42260
                     /gene="yqeC"
                     /locus_tag="OVW45_001128"
     CDS             41490..42260
                     /gene="yqeC"
                     /locus_tag="OVW45_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HCV5525633.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            42308..43933
                     /locus_tag="OVW45_001129"
     CDS             42308..43933
                     /locus_tag="OVW45_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HCV5525634.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EELDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILRVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTDVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(43974..44906)
                     /gene="arcC"
                     /locus_tag="OVW45_001130"
     CDS             complement(43974..44906)
                     /gene="arcC"
                     /locus_tag="OVW45_001130"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCV5525635.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVIAVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(44954..46339)
                     /gene="hyuA"
                     /locus_tag="OVW45_001131"
     CDS             complement(44954..46339)
                     /gene="hyuA"
                     /locus_tag="OVW45_001131"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HCV5525636.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(46392..47603)
                     /locus_tag="OVW45_001132"
     CDS             complement(46392..47603)
                     /locus_tag="OVW45_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HCV5525637.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(47661..48857)
                     /gene="dpaL"
                     /locus_tag="OVW45_001133"
     CDS             complement(47661..48857)
                     /gene="dpaL"
                     /locus_tag="OVW45_001133"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HCV5525638.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQHQSLMEKLALDKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(48915..>49834)
                     /gene="ygeW"
                     /locus_tag="OVW45_001134"
     CDS             complement(48915..>49834)
                     /gene="ygeW"
                     /locus_tag="OVW45_001134"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HCV5525639.1"
                     /translation="LDEGKSQIAHGETVRETANMISFCADAIGIRDDMYLGAGNAYMR
                     EVGAALDDGYKQGVLPQRPALVNLQCDIDHPTQSMADLAWLREHFGSLENLKGKKIAM
                     TWAYSPSYGKPLSVPQGIIGLMTRFGMDVTLAHPEGYDLIPDVVEVAKNNAKASGGSF
                     RQVTSMEEAFKDADIVYPKSWAPYKVMEERTELLRANDHEGLKALEKQCLAQNAQHKD
                     WHCTEEMMELTRDGEALYMHCLPADISGVSCKEGEVTEGVFEKYRIATYKEASWKPYI
                     IAAMILSRKYAKPGALLEQLLKEAQERVK"
BASE COUNT        11953 a        12529 c        13250 g        12102 t
ORIGIN      
        1 acttgcccgt ttttttatcc cttctccaca ccgttgccgc gttgcgcatt ctactttgcg
       61 agtctgctcc agaaaataaa tcaaactcag atcaatgcgt tgtgtttatt taatggggat
      121 ttacgatggc gggacagcat ggggctgtaa cggtaaagcc ctcaccgaag cgagggctta
      181 aaggagaagg gttatgatgc gacttgtcat catactgatt gtactgttac tgataagttt
      241 cggagcttat taacagtcag tctcagggga ggagtcatcc tctcttaccc ttactcacta
      301 aattaggtca aagaatcaac gatgtcaatc agggcgatgc ggggtgcatc gccctgaacc
      361 gaactttttg atagccactt catcgcccac gtttactgcc gtaatagatc ctttttccca
      421 aaaattatct ctgttttgca tgggtttttc gagcacgctc gaagaaaaga tttaaaggta
      481 gatcaatgcg ttgtggttat ttaaagagag cttacgatag ctgggcagca tggggctgta
      541 acggtaaagc cctcaccgaa gcgagggctt gaaggagaag ggttatgatg cgacttgcca
      601 tcatactgat tgtactgtta ctcataagtt tcccagctta ttaacagtca gtcacagggg
      661 aggagcaatc ctcccttacc cttactcact aaattaggtt aaagaatcaa cgatgtcaat
      721 cagggcgatg caggtgtatc gcccttacca ctcccagact ttcgacggtg taaccaccgc
      781 aggaagaggg atatcccact cttcaacggg gagtttttcc accaactgac aatcatgcgc
      841 ataacccacc ggttgcgttt tatagtgctg ccagttttgt aaggtccgat cataaaaacc
      901 accgcccatt cccaggcgct gaccgtactc atcaaaggcg accagcggtg tgatcagcac
      961 gtctaatcgg gaaaggggta gcacgtcacg cacatccagt tttggctcat ggatcttcaa
     1021 cctgttcatc accagttcgc tttgcggatg gtaattcagg aacagcaaat taccggcact
     1081 aaagggatgc aaaactggaa gatatacgcg cttaccggcg cgccagagtt gttctattag
     1141 cggctgggtg tcgagttcac catcaaaaga gaggaataca gcgaccgtat gcgccatcac
     1201 caccggggga taagtcatca tccgggtagc ggcttgttga cccatttcct gctgttgttc
     1261 cggcgttaac gcacgacgac gttgccgaat cattttgcgg atttcttgtc gggataatgt
     1321 cagtgggagt tctggtagtt gcgtcatggc tggtgccaga taagaaggga atctccgaga
     1381 tgccgccgca ggctgtaacc cttgaaccct tggttcaagg tgaatgtgtc gtcgcagttt
     1441 taaggcttct cggacggacc gagcatgctc accaaccgcg gagcgccaca ttcttgtggt
     1501 atgaaatatc ggctcagggg actggcccgc ttgcgaacat ctcagagaaa ttttgtcttc
     1561 acggttactc taccacagta aaccgaaaag tgtcattcaa agttttggtt agttttttcg
     1621 gtgatgcgac cttgttcaag taacgcttgt tctatggtct gctgcagcat ccgaatacgc
     1681 tgttccatac ttgccgcgta gtcacgagtc ttcgcttttt cttgcgctaa ctcatagctg
     1741 atattcaatg cggcaatgaa gaccaactgt tcagtatttg tgactctagt gcgttctttc
     1801 agatcttgca accgttggtt cagatcgtcc gctgcctgat tcaacgcatc cctttggtca
     1861 ggcgggcagt tcacacgcag tgaacggcca aaaatttgga tatcgacggg ttgtgcagac
     1921 atgccacctt cctgctgatt gactgcgctg ccttcgtctt tagacccatg gtctgcgaag
     1981 gggcgacact atagctaccc tgatgagaag agacaagccc ttttctggtc caccagggcc
     2041 caaagtggta gcatatcatg aatattcctc cctttgacga cgaatgctta tgtctataca
     2101 gaacgaaatg cctggttaca acgaaatgaa ccagtatctg aaccaacaag ggacgggtct
     2161 gacccctgcc gagatgcatg gtttaatcag tgggatgata tgtggcggta acgatgacag
     2221 ctcatggctg ccgctacttc acgacctgac gaacgaaggc atggctttcg gtcatgagct
     2281 ggcacaggca ctgcgtaaaa tgcactctgc caccagcgat gccctgcagg atgacggctt
     2341 cctttttcag ctttatctgc ctgatggcga tgatgtcagc gttttcgatc gggctgatgc
     2401 attggcaggt tgggtcaatc acttcctgct tggtcttggc gttacgcaac cgaagctgga
     2461 taaagtgacc ggcgaaaccg gtgaagctat cgacgatctg cgtaacattg cgcaactggg
     2521 ttacgacgaa gacgaagatc aggaagagct tgaaatgtcg cttgaagaga tcatcgaata
     2581 cgttcgtgtt gccgcgctgt tatgccacga cacctttact catccgcaac cgaccgcgcc
     2641 agaagtacaa aaaccgactc tacactaaaa acaaaaaacg taaggagagt gttatgagtg
     2701 agatatcccg gcaagagttt cagcgtcgcc gtcaggccct ggtggagcaa atgcaacccg
     2761 gcagcgccgc gctgattttt gctgcaccag aagtaacacg tagcgccgac agcgaatacc
     2821 cctatcgtca gaacagtgac ttctggtact tcaccggctt taacgaatcg gaagcggtgc
     2881 tggtgctgat taaaagcgat gacactcata accacagcgt tctgtttaac cgcgttcgcg
     2941 acctgacggc ggagatctgg tttggccgtc gcttaggcca ggatgccgcg ccagagaaac
     3001 tgggcgttga ccgcgcactg gcattcagcg aaatcaatca gcaactttat caactactta
     3061 acggcctgga tgtggtttac catgcccagg gcgaatatgc atatgctgat gaaatcatga
     3121 acagtgcgct ggaaaaactg cgtaaaggtt cgcggcaaaa tctcaccgca ccggcaacga
     3181 tgatcgactg gcgtcctgtt gttcatgaaa tgcgcctgtt caaatcgcca gaagagattg
     3241 ccgtactccg ccgcgcggga gaaatcaccg ccatggcaca tacacgggcg atggaaaaat
     3301 gccgtccggg aatgttcgag taccatctgg aaggcgaaat tcaccacgaa tttaaccgcc
     3361 acggtgcgcg ctatccgtcc tataacacca ttgtcggcag cggtgaaaac ggctgcattc
     3421 tgcactacac cgaaaacgag agtgaactgc gcgacggtga cctggtgttg attgacgcgg
     3481 gttgtgaata caaaggttac gcgggcgata ttacccgcac cttcccggtc aacggcaaat
     3541 tcacccaggc ccagcgtgaa atctacgaca ttgtgctgga gtctctcgaa accagcctgc
     3601 gcctgtatcg tccgggaact tccattctgg aagtcactgg tgaagtggtg cgcatcatgg
     3661 ttagcggcct ggtaaaactc ggcatcctga aaggtgatgt tgatgaactg atcgctcaga
     3721 acgcccatcg tcctttcttt atgcatggcc ttagccactg gttaggactg gatgtccatg
     3781 acgttggcgt ttatggtcag gatcgctcgc gcattctgga accggggatg gtactgaccg
     3841 tagagcctgg tctgtatatc gcgccggatg cggatgtgcc agaacaatat cgcggtatcg
     3901 gcattcgtat tgaagacgac attgtgatta ccgaaaccgg caacgaaaac ctcaccgcca
     3961 gcgtggtgaa aaagccggaa gaaatcgaag cgttgatggc agctgcgaga aagcaatgag
     4021 cgtaatcatc gtcggtggcg gcatggcggg cgcgacgctg gcgctggcta tttcccggtt
     4081 aagtcacggg gcgctgccgg tacatttgat tgaagcgacc gcgccagagt cacatgctca
     4141 tccgggcttt gatggacgag ctattgcgct ggctgcgggg acctgtcagc aactggcgcg
     4201 catcggcgtc tggcaatctc tggcggatta cgcaaccgcc atcactaccg tgcatgtcag
     4261 cgatcgtggt cacgctggat ttgtcaccct cgccgcagaa gattaccaac tggcggcgct
     4321 gggacaggtt gtcgaattgc acaatgtcgg gcaacggctg tttgcattgc tgcgtaaagc
     4381 acctggcgta acgctgcatt gccctgatcg cgtggctaac gttgcccgta ctcagagtca
     4441 cgttgaagtg acgctggaga gtggcgagac gctgacgggc cgcgtgctgg tagcagctga
     4501 tggcacccat tcagcgttag ccaccgcgtg cggcgttgac tggcagcagg agccttacga
     4561 acaactggcc gtgattgcca acgttgcgac ttccgttgcg cacgaaggcc gcgcttttga
     4621 acgcttcacg caacatggcc cgctggcgat gttgccgatg tctgacggac gctgttcgct
     4681 ggtctggtgt catccactgg aacggcgcga agaggtgctg tcgtggagcg acgagaagtt
     4741 ttgccgtgaa ctccagtcgg cctttggctg gcgacttgga aaaattaccc acgccggtaa
     4801 acgcagtgct tatccgctgg cgttaacccg cgccgccaga gctattaccc atcgtaccgt
     4861 gctggtgggc aatgcggcgc aaactctgca cccgattgcc gggcaagggt ttaacctcgg
     4921 tatgcgagat gtcatgagcc ttgcggaaac cctgactcag gcgcatgagc gcggagaaga
     4981 catcggtgat tacggcatat tgtgccgcta tcagcagcgt cgacagagcg atcgcgaagc
     5041 aaccatcggc gtcacggaca gcctggtaca tctttttgcc aaccgttggg caccgctggt
     5101 tgtcgggcgc aacatcggac tgatgacgat ggaattattc accccggcac gcgatgtgct
     5161 ggcgcagcgc accctcggtt gggtggcgcg ttgaaggcgg ttttaaggaa taaacaatgc
     5221 aaagtgttga tgtagccatt gttggtggcg gcatggtggg gctggcggtt gcctgcggct
     5281 tacaggggag cggcttacgc gttgccgtac tggagcagcg cgtaccggaa cctctggcgg
     5341 cggatgcacc accacaactg cgcgtttcgg ctatcaatgc cgccagcgaa aaattactca
     5401 cccgtcttgg cgtctggcag gacattctct ctcgtagggc cagttgttat cacggtatgg
     5461 aagtgtggga caaagacagt tttggtcaca tttcgtttga cgatcaaagc atgggctata
     5521 gccatcttgg gcatatcgtt gaaaactcag tgattcacta cgcgctgtgg aacaaagcgc
     5581 agcagtcgtc agatatcact ctgttggccc ccgcagaatt acagcaggtc gcctggggag
     5641 aaaatgaaac tttcctgacg ctgaaagatg gcagcatgtt aacggcgcgt ctggtgattg
     5701 gcgcggacgg tgctaattcc tggttgcgca acaaagcaga tattccgctg actttctggg
     5761 attatcagca tcacgcgctg gtagcgacca ttcgcacgga agaaccgcat gatgcggtgg
     5821 cgcggcaggt tttccatggc gaaggcattc tggccttttt accgcttagc gatccgcatc
     5881 tttgctcaat tgtctggtca ctgtcgccag aggaagcgca gcggatgcag caggcaagtg
     5941 aagatgaatt taatcgcgcg ttaaatatcg cttttgataa tcgcctgggc ttatgcaagg
     6001 ttgagagcgc gcgtcaggtg ttcccactga cggggcgtta tgcgcgccag tttgccgcgc
     6061 accgtctggt gctggtgggc gacgccgcgc ataccattca cccgctggcg gggcagggag
     6121 taaatcttgg ctttatggat gctgcagagc tgattgctga actgaaacgg ttgcatcgtc
     6181 aggggaaaga catcgggcag tacatttacc tgcgccggta tgagcgtagc cgcaagcaca
     6241 gtgcggcgct gatgctggct ggtatgcagg gattccgcga tctgttttcc ggtaccaatc
     6301 cggcgaaaaa actgctgcgt gatattggtt tgaaactggc cgacacgctt cctggcgtta
     6361 aaccacaact tatccgccag gcaatgggat taaacgattt gcctgaatgg ctgcgttaaa
     6421 aatttctctt ctgctgttta tttgataccc atcacacttt catctcccgg ttttttcgcc
     6481 gggagatttt cctcatttga aataaactaa tttcacctcc gttttcgcat tatattttct
     6541 aatgtcatta ttttttgatt tagtgttttt tgacattttt ttagctctta atattgcctt
     6601 attcaaatta gctttctaat ggcatcatct ttatatagaa actggtgtgt tttttggttt
     6661 tttattctgt cgcgattttg gcgtttttta accataagct aatgtgatga tcaattttac
     6721 cttatggtta acagtctgtt tcggtggtaa gttcaggcaa aagagaacga ttgcgttggg
     6781 gaccgggagt ggctccgatg ctgggtttcg tggtgataat ttcaccatga aaagttgtca
     6841 gccccgctta ttcaatgagg acaagatggc acaacagact cctttgtacg aacaacacac
     6901 gctttgcggc gctcgcatgg tggatttcca cggctggatg atgccgctgc attacggttc
     6961 gcaaatcgac gaacatcatg cggtacgtac cgatgccgga atgtttgatg tgtcacatat
     7021 gaccatcgtc gatctccgcg gcagccgcac ccgggagttt ctgcgttatc tgctggcgaa
     7081 cgatgtggcg aagctcacca aaagcggcaa agccctttac tcggggatgt tgaatgcctc
     7141 tggcggtgtg atagatgacc tcatcgtcta ctactttact gaagatttct tccgcctcgt
     7201 tgttaactcc gccacccgcg aaaaagacct ctcctggatt acccaacacg ctgaaccttt
     7261 cggcatcgaa attaccgttc gtgatgacct ttccatgatt gccgtgcaag ggccgaatgc
     7321 gcaggcaaaa gctgccacac tgtttaatga cgcccagcgt caggcggtgg aagggatgaa
     7381 accgttcttt ggcgtgcagg cgggcgatct gtttattgcc accactggtt ataccggtga
     7441 agcgggctat gaaattgcgc tgcccaatga aaaagcggcc gatttctggc gtgcgctggt
     7501 ggaagcgggt gttaagccat gtggcttggg cgcgcgtgac acgctgcgtc tggaagcggg
     7561 catgaatctt tatggtcagg agatggacga aactatttct cctttagccg ccaacatggg
     7621 ctggactatc gcctgggaac cggcagatcg tgactttatc ggtcgtgaag ccctggaagt
     7681 gcagcgtgag catggtacag aaaaactggt tggtctggtg atgaccgaaa aaggcgtgct
     7741 gcgtaatgaa ctgccggtac gctttaccga tgcgcagggc aaccagcatg aaggcattat
     7801 caccagcggt actttctccc cgacgctggg ttacagcatt gcgctggcgc gcgtgctgga
     7861 aggtattggc gaaacggcga ttgtgcaaat tcgcaaccgt gaaatgtcgg ttaaagtgac
     7921 aaaacctgtt tttgtgcgta acggcaaagc cgtcgcgtga tttacttttt tggagattga
     7981 ttgatgagca acgtaccagc agaactgaaa tacagcaaag aacacgaatg gctgcgtaaa
     8041 gaagccgacg gcacttacac cgttggtatt accgaacatg ctcaggagct gttaggcgat
     8101 atggtgtttg ttgacctgcc ggaagtgggc gcaacggtta gcgcgggcga tgactgcgcg
     8161 gttgccgaat cagtaaaagc ggcgtcagac atttatgcgc cagtaagcgg tgaaatcgtg
     8221 gcggtaaacg acgcactgag cgattccccg gaactggtga acagcgaacc gtatgcaggt
     8281 ggctggatct ttaaaatcaa agccagcgat gaaagcgaac tggaatcact gctggatgcg
     8341 accgcatacg aagcattgtt agaagacgag taacggcttt attcctcttc tgcgggagag
     8401 gatcagggtg aggaaaattt atgcctcacc ctcactctct tcgtaaggag agaggttcac
     8461 aattcactgc acgtttcagg aaccatcgct catgacacag acgttaagcc agcttgaaaa
     8521 cagcggcgct tttattgaac gccatatcgg accggacgcc gcgcaacagc aagaaatgct
     8581 gaatgccgtt ggcgcacaat cgttaaacgc gctgaccggc cagattgtac cgaaagatat
     8641 tcagcttgcg accccgccgc aggttggcgc accggcgacc gaatacgccg cactggcaga
     8701 actcaaggct attgccagtc gcaataaacg cttcacgtct tacatcggca tgggttacac
     8761 cgccgtgcag ctaccgccgg ttatcctgcg taacatgctg gaaaatccgg gctggtatac
     8821 cgcgtacact ccgtatcaac ctgaagtctc ccagggccgc cttgaagcac tgctcaactt
     8881 ccagcaggta acgctggatt tgactggact ggatatggcc tctgcttctc ttctggacga
     8941 ggccaccgct gccgccgaag caatggcgat ggcgaaacgc gtcagcaaac tgaaaaatgc
     9001 caaccgcttc ttcgtggctt ccgatgtgca tccgcaaacg ctggatgtgg tccgtactcg
     9061 tgccgaaacc tttggttttg aagtgattgt cgatgacgcg caaaaagtgc tcgaccatca
     9121 ggacgtcttc ggcgtgctgt tacagcaggt aggcactacc ggtgaaattc acgactacac
     9181 tgcgcttatt agcgaactga aatcacgcaa aattgtggtc agcgttgccg ccgatattat
     9241 ggcgctggtg ctgttaactg cgccgggtaa acagggcgcg gatattgttt ttggttcagc
     9301 gcaacgcttc ggcgtgccga tgggctacgg tggcccacac gcggcattct ttgcggcgaa
     9361 agatgaatac aaacgctcaa tgccgggccg tattatcggt gtatcgaaag atgcagctgg
     9421 caataccgcg ctgcgcatgg cgatgcagac tcgcgagcaa catattcgtc gtgagaaagc
     9481 gaactccaac atttgtactt cccaggtact gctggcaaac atcgccagcc tgtatgccgt
     9541 ttatcacggc ccggttggcc tgaaacgtat cgctaaccgc attcaccgtc tgaccgatat
     9601 tctggcggcg ggcctgcaac aaaaaggtct gaaactgcgc catgcgcact atttcgacac
     9661 cttgtgtgtg gaagtggccg acaaagcggg cgtactgacg cgtgccgaag cggctgaaat
     9721 caacctgcgt agcgatatcc tgaacgccgt ggggatcacc cttgatgaaa ccaccacgcg
     9781 tgaaaacgta atgcagcttt tcagcgtgct gctgggcgat aaccacgggc tggagatcga
     9841 cacgctggac aaagacgtgg ctcacgacag ccgctctatc cagcctgcga tgctgcgcga
     9901 cgacgaaatc ctcacccatc cggtgtttaa tcgctaccac agcgaaaccg aaatgatgcg
     9961 ctatatgcac tcgctggagc gtaaagatct ggcgctgaat caggcgatga tcccgctggg
    10021 ttcctgcacc atgaaactga acgccgccgc cgagatgatc ccaatcacct ggccggaatt
    10081 tgccgaactg cacccgtttt gcccgccgga acaggctgaa ggttatcagc agatgattgc
    10141 gcagctggct gactggctgg tgaaactgac cggttacgac gccgtttgta tgcagccgaa
    10201 ctccggcgca cagggcgaat acgcgggcct gctggcgatt cgtcattatc atgaaagccg
    10261 caacgaaggg catcgcgata tctgcctgat cccggcttct gcgcacggaa ctaaccccgc
    10321 ttctgcacat atggcaggaa tgcaggtggt ggttgtggcg tgtgataaaa acggcaacat
    10381 cgatctgact gatctgcgcg cgaaagcgga acaggcgggc gataacctct cctgtatcat
    10441 ggtgacttat ccttctaccc acggcgtgta tgaagaaacg atccgtgaag tgtgtgaagt
    10501 cgtgcatcag ttcggcggtc aggtttacct tgatggcgcg aacatgaacg cccaggttgg
    10561 catcacctcg ccgggcttta ttggcgcgga cgtttcgcac ctcaacctgc ataaaacttt
    10621 ctgcattccg cacggcggtg gtggtccggg tatgggaccg atcggtgtga aagcgcatct
    10681 ggcaccgttt gtaccgggtc atagcgtggt gcaaatcgaa ggcatgttaa cccgtcaggg
    10741 cgcagtttct gcggcaccgt tcggtagcgc ctctatcctg ccaatcagct ggatgtacat
    10801 ccgcatgatg ggcgcagaag ggctgaaaaa agcaagccag gtggcaatcc tcaacgccaa
    10861 ctatattgcc agccgcctgc aggatgcctt cccggtgctg tataccggtc gcgacggtcg
    10921 cgtggcgcac gaatgtattc tcgatattcg cccgctgaaa gaagaaaccg gcatcagcga
    10981 gctggatatt gccaagcgcc tgatcgacta cggtttccac gcgccgacga tgtcgttccc
    11041 ggtggcgggt acgctgatgg ttgaaccgac tgaatctgaa agcaaagtgg aactggatcg
    11101 ctttatcgac gcgatgctgg ctatccgcgc agaaattgac caggtgaaag ccgctgtctg
    11161 gccgctggaa gataacccgc tggtgaacgc gccgcacatt cagagcgaac tggtcgccga
    11221 gtgggcgcat ccgtacagcc gtgaagttgc ggtattcccg gcaggcgtgg cagacaaata
    11281 ctggccgacg gtgaaacgtc tggatgatgt ttacggcgac cgtaacctgt tctgctcctg
    11341 cgtaccgatt agcgaatacc agtaattcac tgattcgact attttctaaa ggcgcttcgg
    11401 cgccttttta gtcagatgac aaagtacaaa agtgctcaga cagtcccctc gcccctttgg
    11461 ggagagggtt agggtgaggg gaacaggccg gcactggcgc gaatatttac cctcaccccg
    11521 gccctctccc tgaaagggcg aggggaaaag cgtgccaaca ttgaagattg agccagtttg
    11581 ttagcaatct caaagatacg tcaacgaatt aatttttctc ggaaaaacaa atggctatag
    11641 cacttgtgac cgatggcagt cgcggcatcg ggcgggcaac tgcattactg ttggcgaaag
    11701 aagagtatac ggtggcggtt aattatcaac aaaacctcca tgcggcgcag gaagtggtga
    11761 acttaatcac gcaagtcggt ggcaaggcat tcgtgctcca ggcggatatc agcgacgaaa
    11821 atcaggtcat cgcaatgttt acagcaatcg atcagcacga tgaaccgcta gcagcgctgg
    11881 tcaataacgc cgggatcttg tttacccagt gcaccgttga aaaccttacc gcagagcgaa
    11941 tcaaccgagt actttccacc aacatgacgg gatattttct ctgctgccgc gaggcggtaa
    12001 aacgcatggc gcttaaaaat ggtggcagtg gcggcgctat cgtcaatgtc tcttcggtgg
    12061 cctcacggtt gggttcgcca ggggaatatg ttgattacgc ggcatcgaaa ggggcgattg
    12121 atacgttaac caccggacta tcgctggaag tcgccgcgca gggggtccgc gttaactgcg
    12181 tgcggccagg gtttattttt accgaaatgc acgccagcgg cggcgagcct ggacgcgtcg
    12241 atcgcgttaa gtcgaacatc cccatgcagc gtggtggaca ggcagaagag gtcgcgcagg
    12301 ccattgtctg gctactaagt gataaagcct cttacgtcac gggaagtttt atcgatttgg
    12361 cgggcgggaa ataaaacagg gaagttgtct gaccggatgc aacaagtatt gcatccggta
    12421 cttcatcgac ttaaagcttc tcgccgttgc tggtaatcac ctctttgtac cagttaaagc
    12481 tcttcttacg tgaacgcgac atatcgccag taccgtcgtc atgtttattc acatagataa
    12541 agccgtagcg tttgctgtac tgcccggtgg tgaacgatac gcagtcgatg cagccccacg
    12601 gtgtgtagcc catcagatcc acgccatcgt aagtcaccgc ttttttcatc tcttcgatat
    12661 gggcgcgcag gtagtcaatg cggtagtcgt cgttgatgct gccatcttct tccactttgt
    12721 cgtaagcgcc aaaaccgttt tcgacaataa acagcggcct ctgataacgc tcatacagtt
    12781 cgcaaagggc atagcgcaga cctactggat caatctgcca gccccagtca gacgctttaa
    12841 catacgggtt cggtacgctg ccttcgaaac cagagatcgc atcgccggtg ccgccttcgg
    12901 ccttcactgc gttggtcatg taatagctga aaccaagata atcgcaggtg ccttcgcgca
    12961 gtacatcgag atcgccgtct tccattttga tgttaaatcc gcgacgctcc cactcgttca
    13021 acacatagga cgggtaatag ccgcgtagct gcacatcggt aaagacgtag cgttcgcgca
    13081 tcgactcctg agcgaacatc acatcgtccg ggttacagca gtagggatag agcggcacca
    13141 tcgccagcat acagccgact ttcatctccg ggttaatgcg acgcgcagct ttcaccgcca
    13201 gggcgctggc gacaaactgg tgatgcagca cctgatacat cgtctcttcc gggttctcat
    13261 gctcggtata aaccacgccg gagcagcagt aaccgaacag cggtgcacgc cagttacgct
    13321 ggttgttaat ttcgttgaag gtcatccagt atttgacttt atgcttatag cgttcaaaca
    13381 ccacttccgc gaaacggaca aagaaatcaa ccactttacg gttggtccag ctaccgtatt
    13441 gctgcaccag atgcagcggc atttcaaagt gggagagggt gatcaccggt tcgatgttgt
    13501 atttcagcag ttcatcgaac atatcatcgt agaacttcag cccttcttcg tttggctgag
    13561 cttcatcgcc ttttggaaaa atgcgcgtcc aggctatgga agtccgaaaa catttgaagc
    13621 ccatttcggc aaatagcttg atgtcttcct tgtagtgacc ataaaaatca acggcttcat
    13681 ggtttggata gtatttgcct ggcaagactt ctttggtgat ttcgcgcggc acgccgtgtg
    13741 cgccaccggt cagaacgtca caaatgctcg gccctttgcc gcctttgttc cagccgcctt
    13801 cgacctgatg agcggcaact gcgccgcccc ataagaaatc tttctgtaag gtgagttttt
    13861 tcactatcat aggctccaga tgagtcaatt cttacaggaa atagtaccat gcagccaaac
    13921 gacatcactt tttttcaacg tttccaggat gacattctgg ctgggcgtaa aaccatcacc
    13981 atccgcgacg agtctgaatc gcacttcaaa acgggtgatg tgcttcgtgt cgggcgtttt
    14041 gaagatgacg gttatttttg cacgattgaa gtcaccgcaa cctcaaccgt aacgctggat
    14101 acgctgacgg aaaaacatgc agagcaggaa aatatgaccc tgactgaact gataaaggtc
    14161 attgccgaca tctatcccgg tcagacacag ttttatgtga ttgaatttaa atgtctttaa
    14221 attgaactca cgcctgttag ttgaaatttc gcatttaatt tcatgatttt taggagatta
    14281 gtctcaaatc agaatttatt ttagctaaca ggtgttcact ggaactattc tcagttacgc
    14341 tggagaggta tacatgtgcg tgtacgtttc tccggagtaa gttatggttc agaagcccct
    14401 cattaagcag ggatattcgc tggcagagga aattgccaac agcgtcagtc acggcattgg
    14461 gttggtgttt ggtatcgttg ggctggtgtt gctactggtt caggcggtgg atctcaatgc
    14521 cagcgccaca gcgataacca gttacagcct ctatggcggc agtatgatcc tgctgttcct
    14581 cgcttcgacg ctctatcacg ccattcctca tcaacgggca aaaatgtggc tgaagaaatt
    14641 tgaccattgc gctatttacc tgttgattgc cggaacctac acgccgtttt tgctggtggg
    14701 gctggattct ccgttagcgc gcgggttgat gattgttatc tggagcctgg cattgctggg
    14761 tattctgttt aaactgacca tcgcgcaccg attcaaaatt ttatctctgg tgacctatct
    14821 ggcgatgggc tggctgtcgc tggtggtaat ttatgaaatg gcagttaagc tcgcggcggg
    14881 cagcgttacc ttattggcgg taggcggcgt ggtttattcg ctcggagtga ttttctatgt
    14941 ctgcaaacgc attccctaca atcatgccat ctggcacggc ttcgtgctcg gcggcagtgt
    15001 gtgccacttt ctggcgatct atttgtatat tgggcaggcg taatgggtat gcctttgtag
    15061 gcctgaaaag acgcgtcagc gtcgcatcag gcatcggagc acaactgccg gattcggcgt
    15121 gaacgcctta tccgatctac gggcctcgtc cctgttgtga taaaggcatc aaatcccaga
    15181 catgataaga cgcgtcagcg tcgcatcagg cgataaatgc ctgatgcgat aaagagattt
    15241 actcttcgag cgaatacggc agcggctcga tatgcaatgt attcgcatcg tcgcgtacgc
    15301 ggaagatgct atccggttcc atatcgttat tcatgacgac ctgtaccacg acctgaccat
    15361 cttccagttt taccgcagcc agcaccgtac cggtacgacg ccagttctcg cccattttca
    15421 gctctaagtc ttcaccagct tccggcagtc ggctggcgct acctgccagc aaccagagcg
    15481 cacgtttatt ggcaccacgg aattttgctc gcgccaccat ctcttgtccg gtataacagc
    15541 ctttcttaaa gctgataccg cccagcgcct ggagattggt cgcctgtggg ataaactgcc
    15601 cgctgttggc ggcatcaatc accgggaaac ccgcttcaat gtttaatgcc agccactgtt
    15661 ggctattgtt cagttccgct tcaccgcgca gtttatcggt cagcatatta gcagtagctt
    15721 catcggttac gatcaggaaa cgttctgccg ggtattcaaa ccatagcaaa gtggtcgcgc
    15781 cttctttaac tacctgtttt tctttcgaag gcagttcact aaagatattt gccagcgcgg
    15841 cgcgcgcctg aaaaccggca acaccaagca gcacatgctc gtcgtctggc gcgatggtca
    15901 ctttagagaa caccgcatat tttttcagtt cagtcagctg cggttcacgc acgctgcgcc
    15961 gttcaatcca cgcaaagcca tcgccgtcgc ggaacagacg taaattgctc cacattttgc
    16021 ctttggcgtc gcaatgggcg gcgagcaggt gctgatcttc tgccatctgg ctgacatctg
    16081 ctgtcacctg accctgcata tatttttcgc tgtccgcgcc agtaatggtg gcaagcgccc
    16141 agtcatcaag cgtcatcagc gtcagcggta aacgggcaga agccgtaggc tgacggggag
    16201 gaaaaggtgt aaaagccata atagagtcct gagttgctta acggcgtatt aatgactaat
    16261 ggtaaaagag ctatgaggca atgcaagtgc tttcactacg cgatctgtgc cttctgcgag
    16321 gcgacttcca aaaaaatgaa gccattgaat tgttgtgaat taatttcgcg atcgcgatgg
    16381 tgtttactga tattcccgta aaagaaggcg aaaaacacgt tacaatagcg gggtactgga
    16441 ttttcgtgag acaggaagaa gatggacatt aacaacaaag cccgcattca ttgggcatgc
    16501 cgccgtggta tgcgcgaact cgatatttca atcatgccgt ttttcgaaca tgagtacgac
    16561 agcttaagcg atgacgaaaa acgcatcttt attcgtctgc tggaatgtga cgatccggac
    16621 ctgtttaact ggctgatgaa tcacggtaaa ccggccgatg cagaactgga aatgatggtc
    16681 cgactcatcc agacacggaa ccgggaacgt ggtcctgtgg caatctgatt tgcgcgtctc
    16741 ctggcgcgca cagtggcttt ccttgctgat tcatgggctg gttgccgctg ttattttact
    16801 catgccctgg ccactcagtt acaccccgtt atggatggtg ttactttcgc tggtggtgtt
    16861 tgattgcgtt cgcagccagc ggcgtattaa tgctcgccag ggggaaattc gcttgttgat
    16921 ggacgggcgt ttgcgttggc aagggcagga gtggagcatc gtcaaagcac cgtggatgat
    16981 taagagcggc atgatgctgc gtttacgttc tgatggcggt aaacggcaac atttatggct
    17041 ggcagccgac agcatggacg aagccgaatg gcgggattta cggcggattt tgttgcaaca
    17101 agagacgcaa agataaagat gacggcaaag catatgcgct aagccgtcag tgagatcagg
    17161 cgtaatgctc tgccatttcg ttgaggattt gctcgcacca gctctgaata cgctcgtcgc
    17221 taaggtcata ctggttagtt tcatccagcg ccagacccac gaacagttgc ccgtcagcaa
    17281 tcaccggttt cgggctggta aattcatatc cttccgttgg ccagtagccg acgaacttca
    17341 cgcctttggt cgagagtttg tcatgcagca taccgagcgc atcgaggaac cactcgccgt
    17401 atcccagttg atcgccaagc ccatacagcg caacaatttt accttcaagg ttcaggtcgt
    17461 cgagctgatc ccagacggct tcccagtctt cctggatttc accaaaatcc caggtcggga
    17521 tacccagaat gagcacatcg tactgctcca ttaatttcgg ggagtcgtcc ttgaggttat
    17581 gtaaggtcac cagttctggg ccgataatat cgcggatttt ttctgccgcc atttcggtgt
    17641 aacaggtgct ggaaccgtaa aaaagaccca tattcataac gtaagcttct cgattctgct
    17701 gtggcttttg cagctagtgt atcagaatcg ctttagtcac ggcacaataa ggcataatgg
    17761 atcaaatgag tgaccataag gggcgcaagt gaaacaggat ctggcacgca tcgagcagtt
    17821 tcttgatgct ctgtggctgg aaaaaaatct ggctgaaaat acgttgaacg cttaccgtcg
    17881 cgatctgtca atgatggtgg agtggttgca tcaccgcggg ttgacgctgg cgacggcgca
    17941 aagtgacgat ttgcaggcat tactggcaga acggctggag ggcgggtata aagccaccag
    18001 ctcagcgcgt ttgctgagtg cagtgcgccg attgttccag tatctttatc gcgaaaagtt
    18061 tcgtgaagac gatcccagtg cgcatctcgc ttcaccgaaa ttgccccagc gtttgccaaa
    18121 agatttaagc gaagcgcagg tcgaacgttt attacaggcg ccattaattg atcagccact
    18181 ggagctacgc gataaagcca tgcttgaagt gttgtatgct accgggctgc gtgtctctga
    18241 actggtcggg ctgacaatga gtgatatcag cctgcgtcag ggcgtagtgc gggtcattgg
    18301 taaaggcaac aaagagcgcc tggtaccgtt aggtgaagag gcggtttact ggctggaaac
    18361 ctatctggaa catgggcgtc cgtggctgct gaatggtgtg tcaattgacg tgttgtttcc
    18421 cagccagcgt gcgcagcaga tgacgcgaca gaccttctgg caccgcatta aacattatgc
    18481 cgtgctggcg ggtatcgaca gcgaaaagct gtcaccgcat gtgttgcgtc acgcttttgc
    18541 cactcattta ttaaatcatg gtgcggattt acgcgtggtg cagatgctac tgggccacag
    18601 cgatctttcc accacgcaaa tttatacgca tgtcgctacc gagcgtctgc ggcaacttca
    18661 tcaacagcat cacccgcggg cgtgatgtct gaaaagaacg ggaagattta tgaagaaagg
    18721 ttttatgttg tttactttgt tagcggcgtt ttcaggtttt gctcaggctg atgacgcggc
    18781 aattcaacaa acgttagcca aaatgggcat caaaagcagc gatattcagc ccgcgcccgt
    18841 agctggcatg aagacagttc tgactaacag cggcgtgttg tacatcaccg atgatggtaa
    18901 acatatcatt caggggccaa tgtatgacgt tagcggcacg gctccggtca atgtcaccaa
    18961 taagatgctg ttaaagcagt tgaatgcgct tgaaaaagag atgatcgttt ataaagcgcc
    19021 gcaggaaaaa cacgtcatca ccgtgtttac tgatattacc tgtggttact gccacaaact
    19081 gcatgagcaa atggcagact ataacgcgct ggggatcacc gtgcgttatc ttgctttccc
    19141 gcgccagggg ctggacagcg atgcagagaa agaaatgaaa gctatctggt gtgcgaaaga
    19201 taaaaacaaa gcgtttgatg atgtgatggc aggtaaaagc gtcgcaccag ccagctgcga
    19261 cgtggatatt gccgaccatt acgcacttgg cgtccagctt ggcgttagcg gtactccggc
    19321 agttgtgctg agcaatggca cacttgttcc gggttaccag ccgccgaaag agatgaaaga
    19381 attcctcgac gaacaccaaa aaatgaccag cggtaaataa ttcgcgtgaa acaacagata
    19441 caacttcgtc gccgtgcagt cgatgaaacg gcagacttgc ccgctgaatt gcctcccttg
    19501 ctgcgccgtt tatacgccag ccggggcgtg cgcagtgcgc aagaactgga acgcagtgtt
    19561 aaagggatgc tgccctggca gcaactgagc ggcgtcgaaa aggccgttga gatcctttac
    19621 aacgcctttc gcgaaggaac gcggattatt gtggtcggtg atttcgacgc cgacggtgcc
    19681 accagcacgg ctctaagcgt gctggcgatg cgctcgcttg gttgcagcaa tatcgactac
    19741 ctggttccaa accgtttcga agacggttac ggcttaagcc cggaagtggt cgatcaggcc
    19801 catgcccgtg gtgcgcagtt aattgtcacg gtggataacg gtatttcctc ccatgcgggg
    19861 gttgaacacg ctcgctcgtt gggcattccg gttattgtta ccgatcacca tttgccgggc
    19921 gaaacattac ccgcggcgga agcgatcatt aaccctaact tgcgcgactg taatttcccg
    19981 tcgaaatcac tggcaggcgt gggtgtggcg ttttatctga tgctggcgct gcgcaccttt
    20041 ttgcgcgatc agggctggtt tgatgagcgc ggcatcgcaa ttcctaacct ggcagaactg
    20101 ctggatctgg tagcgctggg gacagtggcg gacgtcgtac cgctggacgc taataatcgc
    20161 attctgacct ggcaggggat gagtcgcatc cgtgccggaa agtgccgtcc ggggattaaa
    20221 gcgctgcttg aagtggcaaa ccgtgatgca caaaaactcg ccgccagcga tttaggtttt
    20281 gcgctggggc cacgtctcaa tgctgccgga cgactggacg atatgtccgt cggtgtggcg
    20341 ctcttgctgt gcgacaacat cggcgaagcg cgcgtgctgg caaatgaact cgatgcgcta
    20401 aaccagacgc gaaaagagat cgaacaagga atgcaggttg aagccctgac cctgtgcgag
    20461 aaactggagc gcagccgtga cacgctaccc ggcgggctgg caatgtatca ccccgaatgg
    20521 catcaggggg ttgtcggtat tctggcttcc cgtattaaag agcgttttca ccgtccggtt
    20581 atcgcctttg cgcccgcggg tgatggtacg ctgaaaggtt ctggtcgctc cattcagggg
    20641 ctgcatatgc gtgatgcgct ggagcgatta gacacactct accctggcat gatgctgaag
    20701 tttggcggtc atgcgatggc ggcgggtttg tcgctggaag cggataaatt cgaactcttt
    20761 caacaacggt ttggcgaact ggttactgag tggctggacc cttcgctatt gcaaggcgaa
    20821 gtggtgtcag acggtccgtt aagcccggcc gaaatgacca tggaagtggc gcagctgctg
    20881 cgcgatgctg gcccgtgggg gcagatgttc ccggagccgc tgtttgacgg tcatttccgg
    20941 ctgctgcaac agcggctggt gggcgaacgt catttgaaag tcatggtcga accggtcggc
    21001 ggcggtccgc tgctggatgg tattgctttt aatgtcgata ccgccctctg gccggataac
    21061 ggcgtgcgcg aagtgcaact ggcttacaag ctcgatatca acgagtttcg cggcaaccgc
    21121 agcctgcaaa ttatcatcga caatatctgg ccaatttagc gtcatcttct ctataaaaaa
    21181 gagcgtggat tgggtacaat cccgctctta tcaccgcatt ttgactagct caataaaaga
    21241 aatcagacca tgtttgaaat taatccggta aataatcgca ttcaggacct cacggaacgc
    21301 tccgacgttc ttagggggta tctttgacta cgacgccaag aaagagcgtc tggaagaagt
    21361 aaacgccgag ctggaacagc cggatgtctg gaatgaaccc gaacgcgcac aggcgctggg
    21421 taaagagcgt tcctccctcg aagccgttgt cgacaccctc gaccaaatga aacaggggct
    21481 ggaagatgtt tctggtctgc tggaactggc tgtagaagct gacgacgaag aaacctttaa
    21541 cgaagccgtt gctgaactcg acgccctgga agaaaaactg gcgcagcttg agttccgccg
    21601 tatgttctct ggcgaatatg acagcgccga ctgctacctc gatattcagg cggggtctgg
    21661 cggtacggaa gcacaggact gggcgagcat gcttgagcgt atgtatctgc gctgggcaga
    21721 atcgcgtggt ttcaaaactg aaatcatcga agagtcggaa ggtgaagtgg cgggtattaa
    21781 atccgtgacg atcaaaatct ccggcgatta cgcttacggc tggctgcgta cagaaactgg
    21841 cgttcaccgc ctggtgcgta agagcccgtt tgactccggc ggtcgtcgcc acacgtcgtt
    21901 cagctctgcg tttgtttatc cggaagttga tgatgatatt gatatcgaaa tcaacccggc
    21961 ggatctgcgc attgacgttt atcgcgcgtc cggcgcgggc ggtcagcacg ttaaccgtac
    22021 cgaatctgcg gtgcgtatta cccacatccc gaccgggatc gtgacccagt gccagaacga
    22081 ccgttcccag cacaagaaca aagatcaggc catgaagcag atgaaagcga agctttatga
    22141 actggagatg cagaagaaaa atgccgagaa acaggcgatg gaagataaca aatctgacat
    22201 cggctggggc agccagattc gttcttatgt ccttgatgac tcccgcatta aagatctgcg
    22261 taccggggta gaaactcgca acacgcaggc cgtgctggac ggcagcctgg atcaatttat
    22321 cgaagcaagt ttgaaagcag ggttatgagg aaccaacatg tctgaacaac acgcacaggg
    22381 cgctgacgcg gtagtcgatc ttaacaatga actgaaaacg cgtcgtgaga agctggcgaa
    22441 cctgcgcgag caggggattg ccttcccgaa cgatttccgt cgcgatcata cctctgacca
    22501 attgcacgca gaattcgacg gtaaagagaa cgaagaactg gaagcgctga acatcgaagt
    22561 cgccgttgct ggccgcatga tgacccgccg tattatgggt aaagcgtctt tcgttaccct
    22621 gcaggatgtg ggtggccgca ttcagctgta cgttgcccgt gacgatctgc cggaaggcgt
    22681 ttacaacgag cagttcaaaa aatgggacct cggcgacatc ctcggcgcga aaggtaaact
    22741 gttcaaaacc aaaaccggcg aactgtctat ccactgtacc gagctgcgtc tgctgaccaa
    22801 agcactgcgt ccgctgccgg ataaattcca cggcttgcag gatcaggaag cgcgctatcg
    22861 tcagcgttat ctggatctca tctctaacga tgaatcccgt aacaccttta aagtacgctc
    22921 gcagatcctc tctggtattc gccagttcat ggtgaaccgc ggctttatgg aagttgaaac
    22981 gccgatgatg caggtgatcc caggcggtgc cgctgcgcgt ccgtttatca cccaccataa
    23041 cgcgctggat ctcgacatgt acctgcgtat cgcgccggaa ctgtacctca agcgtctggt
    23101 ggtcggtggc ttcgagcgtg tattcgaaat caaccgtaac ttccgtaacg aaggtatttc
    23161 cgtacgtcat aacccagagt tcaccatgat ggaactctac atggcttacg cagattacaa
    23221 agatctgatc gagctgaccg aatcgctgtt ccgtactctg gcacaggata ttctcggtaa
    23281 gacggaagtg acctacggcg acgtgacgct ggacttcggt aaaccgttcg aaaaactgac
    23341 catgcgtgaa gcgatcaaga aatatcgccc ggaaaccgac atggcggatc tggataactt
    23401 cgactctgcg aaagcgattg ctgaatctat cggtatccac gttgagaaga gctggggtct
    23461 gggccgtatc gttaccgaga tcttcgaaga agtggcagaa gcacatctga ttcagccgac
    23521 cttcattact gaatatccgg cagaagtttc tccgctggcg cgtcgtaacg acattaaccc
    23581 ggaaatcaca gaccgctttg agttcttcat cggtggtcgt gaaatcggta acggctttag
    23641 cgagctgaat gacgcggaag atcaggcgca acgcttcctg gatcaggttg ccgcgaaaga
    23701 cgcaggtgac gacgaagcga tgttctacga tgaagattac gttaccgcac tggaacatgg
    23761 cttaccgccg acagcaggtc tggggattgg catcgaccgt atggtaatgc tgttcaccaa
    23821 cagccatacc atccgcgacg ttattctgtt cccggcgatg cgtccggtaa aataagcatt
    23881 acgttatgct cacaaccccg gcaaatgtcg gggtttttta attgtgctgc gcgaaagcag
    23941 acaacaattt tcttgcttca ctattggctg cctgcatcac catccacgga ctgaacgccc
    24001 acggtgtggc atcaataccg tgtaatacat ctgctaaatc acaccattga taatccatca
    24061 cttcatcatc gttgatctgt aacgcactgg tggtgcgtgc ggcaaatacc ggacacactt
    24121 cattttccac aatgccattc ggatcggtgg cgcgatagcg aaagtcagga tagatagatt
    24181 caggaggcgt aatttccacg ccaagctcat aacggcaacg gcggatcacc gcgtcttcgt
    24241 tgctttctcc cagttgtggg tgcccacaaa ccgagttagt ccacacgcca ggccacgctt
    24301 ttttgctcag tgcgcggcgg gtaactaata attgtccttt ggcattaaac agccaactgg
    24361 agaacgcgag atgtaagcgg gtgtctgccg tgtgtgcggc atacttttcc agcgtacccg
    24421 tgggaactcc ctgtgcattc aataaaatga cgtgttccgt ttgcataatt tctcacatgt
    24481 aattctgatc actcatcatt atagataatt gaagagtgaa atgattaacc aagtgagcgt
    24541 ttatcgacaa ccgcccgttt tgagcggatg ccgacaggta aaaaccattt aaaaaagtag
    24601 catcatatat ttgaagcgac tttcaatggc cattgcaaca cagtcagaaa tgacaggaat
    24661 agagagagta aaaagcaaca gcagactgaa tataacgcgc attaaaaaat tcatattatt
    24721 ctccatgctc atttttcttc agcttgcgca tcagaagcca catacgtact gtcctgactt
    24781 ttaaatcttt atcagagacg gattccttca ccaggtcagc atgatgctgg tagccattaa
    24841 agaagacatt cagcacaacg gcgctaaggg ttgcgagcat aatgccgcta tgcagcaacg
    24901 gttgcagtac ggccggtaac ttagaaaaga aatcgtgaga gagcgtcgga gtcatgccaa
    24961 cgccgagact gatcgccaca atatagaggt tgtaacggtt ggtggtgtag ttacagcgcg
    25021 acagaattcg aatccctgtc gccagtacca tgccgaacat caccagccca gcaccgccca
    25081 gcacaaattg cggaatggag gcgaccagca ccgccatttt tggcaccatg ccaaacagga
    25141 ttaatataat tcccgaagaa atacacaccc agcggctatg aacgcgcgtc acgctaacca
    25201 ggccaacgtt ttgtgaaaat gacgtgtgga ggaagctgtt aaacgttccg cctatcattg
    25261 tccctacgcc atcgacacgc agcccgcgaa taatatcgtg tgaagagagt ttacgaccga
    25321 ctatttcacc cagtgccagg aacatcccca tcgactcgat aaacacgatg attaacacgg
    25381 cagtcatggt cagaatggaa acgggatcga aaatcggcat accaaatgac atcggcgtga
    25441 caatcgcaaa ccatgaagca tcatgtagcc cggataaatt gacttcattc atcatccacg
    25501 aaagtaaaaa gccaaataca atccccagta atacggcgac gttggacata aatcctttcg
    25561 catagcgagt aatgagcaag ataaaaatta agacagcaaa ggagatacct aaataaacgg
    25621 gattaccata ttgcggattc cctttacctc cggcggccca gtcaataccc acctgaatga
    25681 tgctaagccc aatagaagta ataaccacac cggtaaccag tggcgggaat aaaggcatca
    25741 agcgaccaat aagtggcgct aataatgtgg tgataaaacc cgcggcgata gtggcaccaa
    25801 atatccccag caggccgata tccgggttca tcccaatggc tatcattggt gttacagcag
    25861 caaaggtcac cgacataatc accggcaggc ggatccccat aaagcggccg ataccgatac
    25921 attgcaataa tgtgacgatc ccgcagcaaa agagatccga gctaatgagc atcgcaatag
    25981 catcttttga gaggcccagt cggtcaccaa tcataagagg aacagcgact gcacctgcgt
    26041 acatgacaag gacgtgttgc agaccgagta tgatcagctt tcctggtgat aatatgcggt
    26101 caacctcatc agttgggcgg tcttgcccag aagatgaggg aagttgggaa tctatggcgc
    26161 tcatcagtgt ttctcctttc gcccgccaga gtaaaaagca accgtttacg ttaatgaaac
    26221 tgtctaaaca catgctcgtg gagtgccagt ttttgcgagg aaggggagtt agagacagga
    26281 attgcggagg caatcacata atcgtgctta aaccacgaaa atgacaagag aacctgacaa
    26341 tggaattatc tgtaattttc tcatattgat atttcattaa tcattctggc atgtcgcact
    26401 ctcgcattta atcgttttta tctggatagc gctcttttga tcggcaatgt tgaaataatg
    26461 aaagcggcat agattgaggg gcaataattt gaactcagtc gcaaaattat aattaaaacg
    26521 gtcatgctac ctaatatatg cccgtttatt gcatcgcgaa atttgagaag acataactca
    26581 ttaaatgaaa gggatgcaaa tgaataagtt tatcgctgct gaagctgcgg aatgtatagg
    26641 ctgtcatgct tgtgaaattg cctgtgcggt ggcacacaat caagaaaact ggccgctgag
    26701 tcacagtgac tttcgaccgc gtatccacgt tgtagggaaa ggccaggctg cgaatccggt
    26761 ggcctgccat cactgcaaca atgccccttg tgttacggct tgtccggtta atgctctgac
    26821 tttccagtcc gatagcgtac aactggacga gcaaaaatgt attggctgta aaagatgcgc
    26881 aatcgcttgc ccctttggcg tcgttgagat ggtcgatacg attgcgcaga aatgcgacct
    26941 ttgtaaccag cgcagttccg gcacgcaagc ctgtattgaa gtctgcccaa cacaggcgtt
    27001 acgactgatg gacgataaag ggttacagtg gataaaggtg gctcgccagc gcaaaacggc
    27061 agcaggaaaa gcgtcatcag acgctcagcc atctcgcagt gcagcgttgc tccccgttaa
    27121 ctcgcgtaaa ggcgcagata aaatttcagc gagtgaacgg aaaacccact ttggcgaaat
    27181 ctattgcggg ctggatccac tgcaagcgac ttatgagagt gaccgctgtg tttattgtgc
    27241 cgaaaaagct aactgcaact ggcattgccc gctgcataac gctattccgg attacatccg
    27301 tctggtacag gaaggaaaga ttattgaagc ggcagaactt tgccaccaga ccagttcctt
    27361 accagaaatc tgcggcaggg tatgcccaca ggaccgtctt tgtgaaggcg catgtacttt
    27421 gaaagatcac tctggcgcag tctctatcgg taatctggaa cgctacatca ccgataccgc
    27481 gctggcgatg ggctggcgtc ccgatgtcag caaagttgtt ccccgtatcg aaaaagtggc
    27541 ggtgattggc gctgggccag cagggttagg gtgtgctgat attctggcgc gcgcgggagt
    27601 tcaggttgat gtctttgatc gccatccaga aattggcggt atgctgactt ttggcattcc
    27661 tcctttcaaa ctcgataaaa cggtattaag ccagcggcga gagatattca ccgcaatggg
    27721 aatcgacttt catcttaact gtgaaattgg ccgcgatatt acctttagcg atttaacttc
    27781 tgaatatgat gcagttttca tcggcgtggg gacttacggg atgatgcgag cagatctgcc
    27841 gcatgaagat gcgcccggtg tcattcaggc tctaccgttc ctgactgccc atacccgcca
    27901 gctcatggga ttgccggagt ctgaagagta tccgctgacg gacgtggaag gtaagcgagt
    27961 cgtggtattg ggcggtggcg atacgacaat ggattgtttg cggacttcca ttcgcctcaa
    28021 tgctgccagc gtgacctgcg cgtatcgtcg tgatgaagtc agtatgccgg gctcgcgcaa
    28081 agaggtggtt aatgcgcgcg aggaaggtgt cgagtttcag ttcaatgttc aaccgcaata
    28141 tatcgcttgt gacgaagatg ggcgtttaac tgcggtgggc ctgattcgta ccgccatggg
    28201 tgagccgggg ccggatggtc gccgtcgtcc tcgtccggta gcgggttcag agtttgaatt
    28261 gcccgccgat gttctgatta tggcctttgg tttccaggcg catgccatgc cgtggttgca
    28321 gggcagcgga attaaactcg ataaatgggg cctgattcaa accggtgacg tcgggtattt
    28381 acctacccag acgcatctga aaaaagtctt tgctggtggt gatgcagttc atggcgcgga
    28441 tctggttgtc actgcaatgg ccgcaggaag gcaggcggcg cgcgatatgt taactctgtt
    28501 tgatacgaag gcatcgtgat gaaatcgtta attatcgtta atccggctga ctgtattggc
    28561 tgccgtactt gtgaagtagc ctgtgtggtc gctcaccctt cagaacagga gttgaatgcc
    28621 gatgtctttt taccccggct gaaggtacag cgactggaca gcatcagtgc gccggtgatg
    28681 tgccatcagt gtgaaaacgc cccttgtgtt ggcgcttgcc ccgtgggggc gctgacgatg
    28741 ggcgagcagg tggtgcagac caattctgcc cgttgtattg gctgtcagag ttgcgtcagt
    28801 gcgtgcccgt ttggaatgat caccattcag tcgttgccgg gggatacccg acaacaaatt
    28861 gtgaaatgcg atctctgtga acagcgggaa gaggggccag cctgtgttga atcttgcccg
    28921 acacaggcgc tgcagttgct tactgaaaga gaactcaggc gagtccgcca gcagcgtatt
    28981 gttgccagcg gtgagaatcc tctgtgaaag aaatctccgc gctgaaaggc gcggagaatc
    29041 tattagattg cccaaccacc cgcgtaaaat gcgaccagtg cgacaggaac gctaatttgt
    29101 tgcccgagta ccagactgaa tgcggtaaat gccgtcaagg aaatcgcaca accaatcgcc
    29161 attggcaaat tggcctacaa ccccatcagc aacgagccga agcccgcgac cagacaggtg
    29221 gcaacaaaca cagctgcggg aggaaaacct gctttgccca gcattcccgg aacgacgata
    29281 acggaataaa ccatggccag aaaggtcgtt aagccagcca gtacttcctg acgaacggta
    29341 ctgccacgag cggtaatttt aaaataatta tccagcgcgc cctgtggctt tggtgcgtcc
    29401 ggtgtttgta ggatgtctcc agacataatg atgtcctcac gaggatgtta aagttttatt
    29461 attagttgcg ttcgtacacc agacgaccat caacgtaggt gcggtagatc gaacggtcat
    29521 cgcccaacgt catcatcacg aacaatttgt cgactaaaga aacagagttg tcatagcgca
    29581 gctgctgtag cggagtggcg gtgggttcca tcaccacgaa atcagcctct ttgccaggta
    29641 aaaagttgcc aatcaaatcg tcaaggccca gagatttcgc tccgccgagc gtggccaggt
    29701 aaaacgcttc atatgccgag aggcgatagc cttgtaattg caacactttg taggcttcgt
    29761 tcagcgtttg cagcatgttg aaagttgttc cggcaccgat atccgttccc atgcccactt
    29821 tgactttctt ctgccatgct tttttcaagt tgaataagcc gctgccgagg taaaggttgg
    29881 aggtcggaca gaaagcaatg ctggatttgg tttcgctgag acgatcccac tctttttctt
    29941 cgagatggac gcagtgagca aagacacagt ttttaccggt cagaccgtac tgatggtaaa
    30001 catccagata accatcatgg tcaggataaa gcgatttcac ccaagcaatt tcatctttgt
    30061 tttcacagag atgggtatgt acccacgtat ccggatattc ttctttcagg cgttgcgcca
    30121 tcgccatctg ttcaggagat gaggtagggg cgaagcgtgg cgtaatcgca tacaacagac
    30181 gaccattttt gtgccagcgt tcgatcagtt ctttgctttg gtgatagctg ctttcggcag
    30241 tgtcgagcag ataatccggt gcgttgcggt ccatcatcac cttaccggca atcatacgca
    30301 tattgatatg actggcggct tcaaacagcg catcaacgga ttgcggatga acagtgccaa
    30361 acaccagcgc cgtggtggtt ccgttacgta aaagctgctt gatgaagaac gccgacattt
    30421 cgcgggcgta ctctaaatcc tcataacgac gttcagtagg gaaggtgtgt ttattcaacc
    30481 actccagcaa ttgttcacca taggccccca ccatttcact ttgcggataa tggatatgtg
    30541 tatcgacaaa gcccggtact atcagtttgc cgcgatagtc gcgcacgcga atagtgtcag
    30601 gaatttgatg ctttccgtct tcccattcgc caaaccattc cacttttccc tgtttaatga
    30661 gtaataaacc atcctcaata aaccgcagcg cagaggcaat ctcttccggg ttatcgaccg
    30721 tacgggtgac atcaataaaa ctgcctcgta ccgctttcaa cgtgtgttct cctgacatca
    30781 ttgactcctt tactgttaat ccatctcttc cgctgaggta ataccaggca gaacgttttc
    30841 ctgtcggtag ccaccaggga ggataatatt taataaaatc gcagttaacc tgccagcaca
    30901 aatagggttt tcaactaata cataaataga ggctggcaat attttaaaaa tttcgggatc
    30961 gtaggagacg ccaagcccta aacctaaaga agtggcgaca ataagtgttt cacggcgctt
    31021 taaaccgttg gtgatgatga tgcgaatccc tgcgatggca atcatggaaa acatcaacgt
    31081 cattgcgcct cccagaactg ccgagggaat ggtcgtgaag aagccgccaa tcatcggaaa
    31141 taagccgagg ataaccagca ttaccgcgat ggttcgcccg acataacgtg aagcgacgcc
    31201 agtcatctga ataaccccat tattttgcgc aaacgtggtt aatggtaatg aaccgacagc
    31261 ggaggcgata acagaaacca gaccatctgc cagcacgccg cctttcagcc gggactgata
    31321 ctcttccccc tgaatggggc ggcgggaaac cattgccgtg gcggtgatat cgccgactgc
    31381 ttccagcacg ctaagcagat aaatcgtgcc aaccaccagg aactgatgga agctaaaact
    31441 aaagccgtat ttgaacggat gcgggatggt gattaacggc aaattgcgca tactgctgaa
    31501 atccaccatg cccaggcata acgatgcgat atagccgaca catagcccaa tggcgatccc
    31561 tcccatgcgt agcaacggac tgcgacagca gttaaagccg atcaccacaa ttaaaaccaa
    31621 taaaccaacg ccgagatgtt cgtaattgcc gaacgtaccg ctgcttttgg ctgcaaatcc
    31681 tccaccaaaa tcgataatgc cgactttaat caggcttaag ccgatcatca gtaccacaat
    31741 accgctgacg gtaggcgtaa taacccgacg taaatagggc aagataaatg aagatccgac
    31801 aaccagaaat gcgccaacga aggagacgcc gagaagcgac gacatgatta actcttcgtg
    31861 aaaaccgtcg cttttcatgc tgctgcccag cgcaatcatg accgtaacaa atgaaaaatt
    31921 gactgactga attgagagta ggccagaacc gacgatgccg tagcggttta cttgtaacca
    31981 ggtaccaata ccagaggcga tcatcgccat agaaacaaga taggcagttg tttcagcgga
    32041 aagctgtaag gccgccccca cgattaacgc gggggttacc atcggaacga aaattgccaa
    32101 cagatgggta atggcaccaa cgagagcctg atgaaaggga gggcgatcct ccagttcaaa
    32161 tataaggtca gaacctgcat tgtttatatc agacatccct tcactccttt taaagtaaat
    32221 ttgatcatct gaattagtaa aataaattcc cacgaatttg gggcgcagga cgccttgaga
    32281 acggcttatc tcattttcgc agtcactatt tagtcatcgt ccaaacagag aaacaggccg
    32341 ccgaagcggc catttattat ttatattttt tccagcgcag tgagtatttt ctccggtgtg
    32401 aaatgccatt cgcgtaacca gatgccgcat gcatcgtgaa ttgcggtagc aatcgccgga
    32461 gctgcgccat ttacaccgat ttccgagatc gatttcgccc cgaacgggcc gactttatcg
    32521 tcgctcggca ccagcacagc gcggaaatca cgcggaatgt caccaatttt cggtgcgccg
    32581 taactgcgta aatcacgcgt taacgggtga ccttcggcgt cataaatgat ctcttcgctc
    32641 atactgtggc cgatagctcg cagggtagca ccgtagattt gtcccaatgc taattccggg
    32701 ttgaccggtg taccgcagtc cagcaaggcg tagaatttat ccaggcggat ttcacccgta
    32761 cgcgtgttga cggcaacttc agcgaagttt gcgccatacg ggaaggcgaa atcaggcgtg
    32821 atataactgc cagttcccac cagtgaacca aagccgatgc cggtttcgcc tttatgggca
    32881 atatccccga aactaacttc gcctttcttg ccgcgcacga cgcccggcgt tgccagttga
    32941 acatctgcca ctgcctcacc caacatttgc gcgccgtgga acagaatttt ctcccgtaga
    33001 ttttccgctg ccaaacgcgc cgcgttaccc gagaagcaag taccggacga ggcatatgcg
    33061 cctttatcaa acaacgcatg atcggtatca ccggagataa catgcacgtc ctgcggtggg
    33121 cagtgcagca cttctgctgc cagtttcgtc actacagtat ccagaccagt accaatatcc
    33181 gcaccgccag aatgaacgat aaaggtgccg tccgattcca gtttgatcat gcagttagcc
    33241 tgatcgatat ccgggatacc cgatttctgc atgataatgg cgacgccgcg accgatgtgc
    33301 cagtcaccat tttgcggttt cggtgacgac cattggatca tctcgcgacc ctgacgcagg
    33361 atctcttcca gtgcacagct ggcggcggaa ggaacggagg tcggcgcttt accttcaccg
    33421 attgcaccga gaattttcag ctcttgccct tcgtgtaccc ggttacgttc gataatttcc
    33481 agttggtcga tctgtaactg ttcagccagt tccgctaatg ccatggtgat agcgaagtta
    33541 cctttcggtg cgccataacc ctgataagca ccatttgggc aaatgttgct gtagtaggtg
    33601 gtgacctgga aatcgacgtt atcgcacgga tataacggca gcgacagcgc cggtccgtta
    33661 cacggtacgg tgagtgagtg gttgccgtaa gggccagtgt tggcgcggaa atccatcttc
    33721 actgccgtca ggcgaccatc ttttttcgct cccagtttga cggtgacttt cgcgacgtga
    33781 cgagaggtgt tagcaataaa ctcttcttca cgggtgtagc ggaatagtac cggacgcccg
    33841 gtcacgcagg ttgcccaggc gcacacttct tccagcagga tgtcctgttt ggaaccaaaa
    33901 ccgccgccaa ctcgctcttt aatgacatga actttatgct gtttcatgcc cacgaggcgc
    33961 gcgacctggc ggcgtaagtg ccatggtacc tgggtggagg cgtggataac cagacgatcg
    34021 ccgtccatcc gggtaaagca gatatgtgtt tcagtcgggc actgctgtgc ctgcgttgag
    34081 ttataggttc gctcaatgat cacatcggca tcggcaaagc ctttgtccat atcgccaata
    34141 tgaccatgaa tactggcggc gatatttttg cgagggcgag aaccgatcgg gaagttgatg
    34201 atcatatgct cgccgcgctg ggctgcatgg ctgttatcgt cttccagagt atctggcgca
    34261 ccagcaacat acaccaccgg ttcatcgtgc acgacaggcg catcttccgc cattgcttcg
    34321 tcgatcgaca ttaccggctt aagcacttca tattcaacgt cgatgagctt cagtgcttcg
    34381 agcgcaattt cttcactttc tgcgacgacc gcagcaacac gatcgccgac gtgacgcatt
    34441 ttcttgccga acatacggcg gtcaagcggt gacggttccg gtgcgctctg accccccggg
    34501 gtatagtaga tatcagggca attcaggtga gtaataacgt gaacgacgcc cggtaaggct
    34561 tcagctttgc tgacatccag atgagtaatc agtgcgtgag cgtgtgggct acgtaacatt
    34621 ttaatgacgc aggcgtcagc cgttacgcag tcttcaacat agcagggttt cgcctgcacc
    34681 attttcgcgg catcagtttt aggataatgc ttgccaatga cgtcaaggtc gtcacggaaa
    34741 gtcggagcga tatcaatggt ggcctgcgga ttatttttac gtgcaaccgc cagttcaatg
    34801 acctgatagt attgttgcca gcctgcatcg cggctgaaca aaccagaaag cgcgtcgtcg
    34861 atctcttcgc gggtaggtgc ggcgatgcga tcgagaagat cggtgataat cagagctgca
    34921 gctggatcgt tataaccaga ctgcaccacg ccaacatcaa ccattgcctg ttgaaccaga
    34981 cttaactcgt tccatttacc cagagattct gcggtacgaa tatctgcctt ctctaactgt
    35041 gcggcaataa gcaaggacgc gttaacgata ttaccgttaa agattattgc gtcagaaccg
    35101 gcaaacccga agccatcatc actgttgcgt acagagtgca ttcccatgtt aaacaacagc
    35161 ttttgcacgt tttcgcctgg attaacggtt agctcctgag gcgcgccatt taaagtaaag
    35221 tggatgatca tacggcttcc tccccagcct gttggcagtc ggcatacagg tcggctacca
    35281 gaactcccgt gatatagcgt ttataggcca cgctgccccg caaatcttcc tgcgggaaaa
    35341 tggcgttggc gacagcctgt tcaagtgcat tgccttccag attttgtttt tcgacatcgt
    35401 gcagacgcag tgctttactg gcgacgccat ccagcgcaat tcgcataccg tcgtggtctg
    35461 tcattgcaac ggctgccgtc acgacggtta aacctgcctg agaacggcta attttgcggg
    35521 tcgcacaggt gcgatacgga tctttaatga taatttcggt tagcaggcga tcgcatgggc
    35581 aggccaggta gtcctcgatt gacagcgttt cgccgttgcc aaaaaccagt tcagcatcca
    35641 gtgctagcag gacgggaagc agcactgact cttcctgccg ggcggcgatt tcaccaccaa
    35701 tggtcgactg attacgaaca tggcgtgagt aaacaaaacc gagggcttca cgcagtgctg
    35761 caggaataaa gcgcgcatca cgcagtggct gcaagcgaga cattgcacca atccgcagtg
    35821 caccgttatc ccagtcaacc cagtccagtt ccagatcctg taaggaaatg gcaatctttt
    35881 tatcggtacg ggttggtgta gcattgagtt tgctgccccc ggcgaaccag acggcttcat
    35941 cctggtagcg gcgcttcagt tccagcgcct gttcgacaga gtcgggcctg aaaaattgtt
    36001 caatcatctt ctttccttat ttaaccctct tccctgcggg aagagggcat ctgtcattta
    36061 tgccagcgca tccatccgac gccacatact ggcagcggct tttctggctt gtgcataaat
    36121 ggaatcgcaa tcgaagttaa actgacggtc ttcatagacc atcacaccat tgaccatcac
    36181 gctgtgaacg ctgcctgagc ccataccgaa agcgatatgc ccggcgatat tgtctgccag
    36241 cagcggcgtc ggcgagttgt aatcgcaaat ggtcaaatca gctttgtaac cggcttccag
    36301 aagcccaaat ttcgcgccaa agttgcggct catcagttcg ttaccgttag tcagggcttt
    36361 ggcaaaactg tcaggccaca gcggaccacc cgcatcgcga tgtttaaaga aggcaaattt
    36421 catctcttca aacatgtccg aaccaatgcc gtccgttccc aatgccaggt tgcggatgtc
    36481 gctaaggtga tggttgtagc cgacatggtt gttcatgttt gaacgggcgt tatgcaccag
    36541 gaacgcatcg cgctgattga gtagggcgat gtcatctttc gacaagtaca gcccatgagc
    36601 gaccagcgtt ttgctgtcga tgaggtcgaa ttgcgccagt cgtgccagca ggtctttgcc
    36661 gtaccagtgg tgactgtagg aaacgtcgta aaggtcttcc gcagcgtgaa tatgcaaacc
    36721 acggcctgtg gctttcacgg cttcacgcag catctccaga ccggcatccg gcacggtaaa
    36781 cggcgcgtga gcaccgatat gtgcttccac cagatacggc tcgcttgtcg ctttcttcgc
    36841 ctcatcaatc aaacgggcga aacggatgtt ttcttctaca ccttcctgca actctttgat
    36901 gccgttgtta cggtcagtag tttcaaaaca ggtcatcgcg cgcaggccaa cttttaaaaa
    36961 tgcgtcgcgc aatgtggaga gcgacccgcc gatatacgcc ggcgaggcat ggtgatcgat
    37021 aaccgatgta catccgctct taatcgcttc cagggaacaa atcagtccgc tgtaatagag
    37081 cgactcttca tcaagggcgc gatcgagccg ccaccagaga tttttcagcg ttgagatgaa
    37141 atccgggcaa ggggcgatgt ttgccataat tccgcgggaa agctccgagt aaaaatggtt
    37201 gtgcgagcag acaattcccg gcatcacaat ccgaccatgc atctctttgt agctggcatc
    37261 ggggtagcgt tgcgtcaggg catcgccgat agcgacaatc acgtcatttt cgatagcgat
    37321 atcaacgcct tcctgcactt tcgccgggtg taactgcact gcggtgacat tcttcagaat
    37381 caacatgatt acacctccac gcggcccagc agataatgat gatgctggtg gacatggctg
    37441 atgatgcggc acatatcgtt cagctccggt ggtacgttgt taaactgacc ttcgctgtcg
    37501 atgtttaaca cccagctttg gttattcaga cgtactcgta cccggcaatc ttccacaagg
    37561 aagcctgggg tgctgctgtt atcaaagtct tgcgccaggc tgaagacggt gattttgtct
    37621 tcgtatggtt taccgttcca cggacagaac tgagcgcagt tgccgcattc gttacagtaa
    37681 gcgtcgaggt gcagcgtctg gaatctattc tggaagccag ggactgcaat ggagacgttg
    37741 gcgcggttcg ggcagacatc cacacacttg ttgcaaacgt agttacattc gaggcagcga
    37801 gcggcttcct gggcgacaaa cgcgtcacga tcgtcactgt tcaccagcgt gatagagata
    37861 tcgcctttac gttgatagat ttccgctgga ttgacgttgt tccagtattt atcgttctgg
    37921 tgggaacgga tattttcccg actaaggatg gcatcggtcg cccgacgcgc ggttccgaca
    37981 gcagcgacaa tggaggatgg tccgcgctgt acgtcgccga tcataaagac gtcagtcaga
    38041 cgagtttcgc cattatggtc gacgtctggc cagccgtttt tgtccagcgg cacgcccatc
    38101 gcattcaggg cttcagtatc ctgctgttca ccaatggcgg tgatcaggct gtctacatgc
    38161 agtgttactg tttcgttggt ttcaaccgga cggcgacgac ctttctcatc cggttcgcca
    38221 agcgacataa cgcgcaaggt taaggtgcca tcagcatcga aacgttccgg attattcagg
    38281 aaacggaact ctacgccgtc gtgcaacgct tcttcatact cttcgcgcca tgcgggcatc
    38341 tcttgtagtg aacgacggta aacgatcgtt gctttttcta cgcctggaac gcgtaacgcc
    38401 gcacgagcgc agtccattgc ggtgttaccc gccccgacaa cgaccacatg tttgcccagc
    38461 ttgagcgctg tacccttgtt gtattcacgc aggaagggga gtgatttcca gacattttgg
    38521 ttgtcgcccg ccagtttcac accgctattt ttatcagtgc cggtggcaat cagaacatag
    38581 tggaagccct gattttttaa ctgttcaacg gttaaatcgg gtgagcagcc atactcaaat
    38641 ttcacgccgt gagcggcaac aaaatcgata tcgtgctgaa ttaactctgc aggaatacgg
    38701 aactgaggaa tgatattttt caccacgccg ccagcattgg cttcgcgttc aaacagcgta
    38761 accggatggc ccgctctggc aaggaagtaa cctgctgcca gacccgccgg acctgcacca
    38821 atcacggcaa ccggatggcg tgaaccagaa ccggcgggtt tgtgccagcg ttgcttatat
    38881 tcatcccaac ctttttccag cgcgactttt ttcagttcgc ggatattcag cgcactgtcg
    38941 taatccaggc gggtacagtt gtattggcac tggtgatcgc aaatatgacc ggtaatggca
    39001 ggtagggcgt tgcgttggta gatgagttcc agcgcgtcgg catagcggtg ttcgccaagc
    39061 agacggatgt attccggaat atcttgcttg atagcgcagg cagtaacaca gggggcaacg
    39121 tagcaatcgg tcagcggcag gtcttctgcc acttcaatac gctcttctgg cttccagtgt
    39181 ttctgggtgt attccatggt taacgcgtct gctgccagtc tgttcagtcg ttcgacgtca
    39241 acatggtcaa gtccccaggc gtcggagcct tccagctcgc gcatgcaggc acttaagcgc
    39301 agatagccgc caggtttcag caggtcggtt gccatagtaa tagggcgaat acctgtatca
    39361 aaaatatcgc ggatagtcag ctgactggca ccaccggaat aagaaatggg cagtttgccg
    39421 tcaaaggcgc gagagagaac tgctgcaaca ttgatggaga gcgggaacag cgcacggcct
    39481 gacatataca tctcttcacc aggcagtgcg cctttattgt tgatggtgcc gagagtgtta
    39541 gtcagtttta cgccaaagcc gagtgatttt tcttttgcca gtaccatcag gcgctccagc
    39601 atttccagtg cttgcgtcag cttgaggtcg tgatcaaatg actcttcttt taagcctatg
    39661 taaccgaaac cgcagacatc gaggatctca cgaacacgcg cgtaccccag taaggtcggg
    39721 ttaagtttga caaaggtgtt gagccctttt tcttccagca tgtagcggca aatggcttcg
    39781 atttcatgcg gaggacagcc gtgcatggtg gagagggtga cgccctgcac catactggtg
    39841 gggatgcgag cgggtaaggc ttgcaagttt tcgcgttttt cctgcaatcc gtggcgggct
    39901 aaaaatgcgt catcctggag taatttattc agcgtatcgc gatattgagc gaatttcgga
    39961 tggtcagatg cgtccatcat attgtcgatg aactgttgca tcggcggttg cttaatacct
    40021 tcgaggttgt aaccgacgct catattaaag atgaacgatt taccggaatc agaaggctgt
    40081 aacatcgctt cgagaaggtg cagggcaaac caggctttga ggtattcatc ccaggcttta
    40141 agcaaggtaa attcggtaga ccattcggtg ttaaagcatt cgtcttcggc atcaatacag
    40201 ggcttttcca gctccaggcg gtcaagaatt tggacggttt ttagttcgat gaagcgtccg
    40261 ccagtcagcc aggaagtgac aatattttgc gcgagctgcg tgtgcgggcc agcggcaggg
    40321 ccgacgggag tggcacaggt ttcaccgaag acgctaacag ttttaccttt tacaggtgag
    40381 taaaactgtt gctcgggaat accaaagatt gagcgttgtt gttggtattc atcaaatatg
    40441 cgcgtcaaaa gttcctcaaa cggaatggga cgcataatat cccccataac cctctcctta
    40501 gcttatacag atatcattga gtgaccggtg ttgagaaatc cggtggttta gggggataga
    40561 gcaacaatcg caccactctt gccaggtttg gcgggaaata taaaaaaatg tgaggcggct
    40621 cgaaacttaa aggctttttg tgaatgaact cacaggtgcc aaatgagaat taaaatcact
    40681 cctgaaaatt attattgtca gcccatttat cactttttct cttttctcaa aatcagtgat
    40741 tccttatcat tatgataaag agtgctggag agattaaata tttgaacgca atcgtttaac
    40801 tacattttct aaattttagt ataccttttt tgcgctgtaa taaagtcgtc cggagtatca
    40861 atatctaaaa ttatttctgc attttctatt tcgacggaat aatgatctcc cataaggagt
    40921 gcctgacgca tattggtgac attgggtcgc tggattgcct gcatcaggca tggttttgac
    40981 actaaaatcg gatggccggg gatgccatta tggagtggca gtattgcgcc atcatttcgt
    41041 aacgaccaga tttttctaaa aatatcgatg gtgagggttg gcatgtcacc gtgggtgaga
    41101 aaacaatgtt ctgtttgcac tgcgggtact gcggccttga cggatgttag taaaccctgc
    41161 gcataatctg ggttgtgaat aatagtgata ttgctctggt tcgcataacg ttcgtgcagt
    41221 tcattaccac gatagccggt gactaaaata attcggctac aaaactgcaa cgcatttttg
    41281 atacttgtat caagaattgt tccctgttcc caggggaaca tcattttcca ttgccccatt
    41341 cttgatgata atccggcagc ggtaattata cagtcgatgg ctgacatagt ggttccatta
    41401 tttatagggg ctaataacgc ctgataattg cgttacttcc tgatggatta agttggggat
    41461 aatttatccc agagaggtca taaagactcg tgaaaagtat agttgaccca tcggcattag
    41521 tcattgattt aggtgcgcag aaacgtccca ctgtgatttc tgttgttggt gctgggggga
    41581 aaaccagctt gcttttttgg ctggcagaac tgctccaggc tagcggcagg cgtgtgttaa
    41641 tcacgacgac gacacatatg tttatgccaa cgtctcactg gcccgtggtt ttctgtcgtg
    41701 atcccgccat gcttcctcat gcgtctctta catcccccat ttcattttgt tttcacagct
    41761 ggaaagcgaa ccagggaaaa gtgcagggat ttacgccaga agcgattgat gcactggtgc
    41821 aacgaccaga gtgtgacgta attctcattg aagcagatgg ctcgcgtgga atgccgttaa
    41881 aagcgcctga tgagcacgaa ccttgcatac ccaaaagcag ttgctgcgtg attgctgtga
    41941 tgggaggaca tactttgggc gcgaaagtga gcacagaaaa tgtccatcgc tggtcgcagt
    42001 ttgctgatat taccgggtta acacctgatg caaccttgca actgagcgat ctcgttgcgc
    42061 tggttcgcca ccctcagggg gcgtttaaaa acgtaccgca aggttgtcgg cgagtctggt
    42121 tcattaaccg tttttctcaa tgtgagaatg cgattgcaca aagcgagctc cttcaaccgc
    42181 tgcaacaaca cgacgtagag gcaatctggc tgggcgatat acaagaacat cctgcaatcg
    42241 cgcgcagatt tgtgaattag cggcccgggt attccgggtt actgaaagga tatccattga
    42301 ggttggtatg aatattttca cagaggctgc aaaactcgaa gagcaaaatt gtccgtttgc
    42361 gatggcgcaa attgttgata gccgaggctc gactccccgc cattctgcac aaatgttagt
    42421 gcgcgccgat ggttctatcg tcggtacaat tggtggcgga atggttgagc ggaaggtgat
    42481 tgaagagtca cttcaggcat tgcaggaacg taagccgcga ttattccatg gacgtatggc
    42541 tcgtaacggt gcggatgctg tcgggtcaga ttgtggaggt gcaatgtcag tatttatcag
    42601 cgtccatggt atgcgtccac gtctggtgtt gattggcgcg gggcatgtca accgggcgat
    42661 agcccagagt gcggcgctat taggatttga tattgccgtt gccgatattt atcgcgaaag
    42721 cctcaatcct gaactattcc caccatcaac cacgcttctc catgctgagt cgtttggtgc
    42781 ggcagtggaa gaactggata ttcgccctga taattttgtc ctgattgcca cgaataatca
    42841 ggatcgtgaa gccctcgaca aactcattga acagcccatt gcatggttgg ggttgctggc
    42901 aagtcgccgc aaggttcagc ttttcctgcg tcaattgcgt gagaaaggcg tggctgaaga
    42961 acatattgcc cgtttacatg cgcccgttgg ttacaacata ggtgcggaaa cgccgcagga
    43021 gatcgccatc agcgtgctgg cagaaatatt acgggtgaaa aataacgcgc cgggtgggct
    43081 gatgatgaaa ccttctcatc cttccggaca ccagctggtg gtgattcgcg gtgcgggaga
    43141 tatcgccagt ggtgtggcgc tacgtctgta tcatgcgggt tttaaagtga tcatgctgga
    43201 agtggaaaaa ccgacagtga ttcgttgtac cgtggcgttt gcccaggccg tgttcgatgg
    43261 cgaaatgacg gtcgaaggcg tcactgctcg cctggcaacc agctctgcgg aagcgatgaa
    43321 acttaccgaa cgcggattca tccctgtgat ggtagatccc gcctgttcat tgcttgatga
    43381 actgaaaccg ctttgcgtgg tggacgctat tctggcgaaa cagaatttgg gaacgcgggc
    43441 agatatggca ccagtaacaa tcgcgcttgg gccgggcttt actgcaggga aggattgtca
    43501 tgcggtaatt gaaacaaatc gcgggcactg gctcggtcag gtgatttact ctggttgtgc
    43561 gcaggagaat accgatgttc ctggcaatat tatggggcat accacccgac gggtgatccg
    43621 tgctcctgct gcaggcatta tgcgatccaa cgtgaaatta ggcgatctgg tgaaagaggg
    43681 cgatgtgatt gcctggattg gtgagcatga aattaaagca ccgttgacgg ggatggtgcg
    43741 tggcttgttg aacgacggcc tggcagtggt tggtggtttt aaaattggtg atatcgatcc
    43801 tcgtggtgaa accgctgatt tcaccagcgt ttctgataaa gcccgggcga ttggcggcgg
    43861 cgtacttgag gcgttaatga tgttgatgca tcagggcgtg aaagcgacaa aagaagtgct
    43921 ggaagtggct taaatgaaaa tggccggatg gtattaccca cccggcctta aaattactga
    43981 caaatcaccg ttccggtttt gccttcaatc ccttctttcg ctttgctcaa tacggtaatc
    44041 agtgcttcac gacctgcgcg agagcgagca aacgaggcgg cagcttccac tttaggcagc
    44101 atagaacctt tcgcaaaatg accttcttcg ataaagcgtt cagcatcact taatgacagg
    44161 cgatcgagcc actgttcatt ctctttaccg aagttaatgg ccactttttc taccgcagtc
    44221 agaatgatca gcatatcggc atcaatcatt tctgctaaac gggcgctggc ccagtcttta
    44281 tcgataaccg cgctggcacc acgcagatgg ttaccttcac gaataacagg gataccacca
    44341 ccgccaacgg cgatcaccac ctggcctgca tctaccagag ctttaaccgt ttctttttca
    44401 atgatatcaa ccggttttgg cgaggcaaca acacggcgat agccgcgacc agcatcttct
    44461 ttcagagtgt aaccctgttt tgtcagttgc tctgcttctt gctcggtaaa gaacgaaccg
    44521 atcggcttgg tcgggttgag gaatgccgga tcgttagcgt cgacttcgac ctgagtaacc
    44581 agcgttgcta caggtttatt aatgccccga gaaagcagtt cttctcttaa cgcgttttgt
    44641 agatcgtaac caatataacc ctggcttaag gcaacacaga cagacatcgg cagcattggc
    44701 gagtgtgctt cagtttttgc ggcagcttca aaggcttggt taatcatgcc cacctgcggc
    44761 ccgttaccat gagtgacgat aacttcatgt ccttgggcaa ttaaatcaac aattgcctga
    44821 gaagtaattt ttaccgcttt catttgtccg gccaggtcat cgcccagcgc attcccgccc
    44881 agggcgagaa caattttctt actcattttt cctcactcat cattcttgat tgtgtataga
    44941 tccctgacag tggttagagc acgggaggga caaacggttt gcgtcgcagg aaacgacctc
    45001 gcccggcttt gcctgtaaag gtgccgtcac agaaaatcgt ttcaccacgg gataaggttc
    45061 tgacaatcgc gccctgacag gtaaaaccct cccatggcga gtagtcggcg ttgtcgtgga
    45121 gatggcgatg ctgaatttgt tggctctgac gtgggtcgat aatcaccacg tcgccatcgg
    45181 aaccgggcgc taataatcct ttttgcggcc acaggccaaa cagcctggcg ggcattgcgc
    45241 tggttaattc aacaaagcgt tccggtgtta tacgtcccgt catcacgcca ctggaaaaca
    45301 gtaactgcat gcggttttcc acaccgggta agccatttgg gcagcgactg aaatcgcctt
    45361 tagaaatttg caggcgttga gccatcgaga aggtgcagtg atcggtcgcc accacgtcaa
    45421 tcgcaccatc gctgatgcca caccacagtt tgtcctgctc gcgtacgtta cgcagcggtg
    45481 ggctaagaat gaacttcatg ccatcttctg tatcgtaact gcgttcgtcc aacaggagat
    45541 attgtgggca ggtttcaacc cagactggct ggtgattcgc acgggcaaga cgcagataat
    45601 ccagacctaa gccgttagac aggtgcacga tatagagcgg ggcgttaccg gcaatttgtg
    45661 ccaggttaat catgcgggcg atggcttccg cttcgcattc cagagggcga ctcaaggcat
    45721 gatagcgcgg cgcggttaac cctgcggcga taaattccgc ccgcttgctg gcgatagccg
    45781 catcattttc cgggtgcacg gtggtcagcg cgccggattc atgcaagcgg cgtaatgcct
    45841 gcaaaacctc gtcatcgttg agtttgtatt gataggttaa atagagttta aaactgctca
    45901 gtccttcctc gaccatcatc gggatttcgt cgaggattgc gtgattaatg tgctggatca
    45961 caccgtgaaa gctgtaatcg atgaccgctt tatgggcggc ataaccacga taaacctcca
    46021 gttgatggcg taaccgacag ccgtttgggc caaatcccat atggtcaata atggttgttg
    46081 taccgccaca cgcagctgcg cgggtaccgg taaaaaaatc atcacaactg cgcgcgatgc
    46141 cgacatcaat attgaaatgc gtatggacat ccacgccgcc agggaaaacg taacagccag
    46201 tggcatcaat ttcttcatac gggagctgcg gcgaaatatt gttgcccaac tggcgaacaa
    46261 tcccgctttc aatcagcaaa tcctgtttgg cttgtccatc tgcgttaacg acagtgccgt
    46321 ttttgatcaa tacgcgcata gcaaactcca aaaaccctcc ggcgaaccgg aggggaataa
    46381 gtgaagagta attactctgt tgccagccag cttaacggga ttgcagcgta catcgcggca
    46441 caggtcacca ggtgagattt ccaggttttt tcgttaggtg catgcgcttc aggttcttta
    46501 cccgggccaa agccgatcac cggaatgccg tgacggccca tgatagatac gccgttagtt
    46561 gagaaggtcc acttatcaac aaccggcgct ttgccaaaca gcccttcgta ggcattcacc
    46621 agtgctttaa cggtgaagtg atcttcttcc actttccagg tcgggaagta gcattcggtt
    46681 gggtaaacca ggccagtcca ggacggacgg tcgtagttgt acatagaaac aaccgcgtta
    46741 gctttctgta ctgcaggcag ggcgcggatt tcgtccagcg cgccttccca ggtttcgccc
    46801 caggtcagac ggcggtcaat agagacggcg cagctatctg ctacagcgca acggcttggg
    46861 gatgtgaaga agatttcaga aacggtgagg gtgcctttgc cgaggaattc gtcataaccc
    46921 agacgttggg agagttcttg taattcgcca agaatcggac ccattttgaa aatggcgttg
    46981 tcaccgcgtt ctggcgcaga accgtggcag ctaacaccct gaacatcaat acgaatttcc
    47041 atacgaccgc gctgaccacg gtatatctgg cagtcggttg gttcggtact gaccacaaat
    47101 tccgggcgaa tgccggattg ttcaataatg tactgccagc acagaccgtc gcagtcttct
    47161 tcctgcacag taccggtaac cagcagggta tattcatctt ccagaccgag gtctttaatg
    47221 attttaccgg cataaaccat agatgccatg ccgccttcct ggtcggaagt accgcgacca
    47281 ccgattagct catcagtttc catgccttcg tacggatcga agtcccagtt tttgatgttg
    47341 ccaatgccga cggtatcgat atgagcgtcc attgccacca gacgcgggcc gtggccgata
    47401 taaccgagaa cgttgcccat cgggtcgatt tcaactttat cgaagccgac tttttccatc
    47461 tcttctttaa tacgatgtac tacgcgtttt tcgtcgcagc tttcactggg aatagcaacc
    47521 atgtcgcgca ggaagcgagt catgtccgcc tggtaatctt ttgctttttc aagaatcagt
    47581 ttgaatggaa tattcttagc cattattttt ctccagattt tttactcccc gttttgcagg
    47641 gagtagttcc aatagggtga ttaaggtgct acagcgtgtt tgccttccca gacaacttcg
    47701 cggtagtgct tcacgtcggt gtcgccttca gtgctgataa ccagcactac ggcatctttg
    47761 tccagcgcca gtttttccat caggctttga tgttgcgggt gataatgaac cgctgcgaga
    47821 acgcccaaac cgacagcgcc ggattcaccg gagatgatgc gcgggtcgtt gccgtacgga
    47881 ttacccagca cgcgcatacc taatgcggca acgctgtcct ggcaggagat gaattgggtg
    47941 gcgcagttac gtaggatttc ccagcccagc gggttaggtt cgccacaggc caggcccgcc
    48001 atgatggtgg ccatatcacc gccaacgttg acgatgtcgc ctttgacgcc ggagcgataa
    48061 atacagtcag ctttgtcagg ttcaacaata atgctgtgca gattttgcgg gctgtagacg
    48121 tcgaccagat aacccagcac accaccggcc attgctccga caccggcttg cagcagaacg
    48181 tgcgtcgggg ttacgcccat ttcacgcatt tgctcgacgg cttcatctgc cagggttgcg
    48241 tagccttgca tgatccaggt tgggattttg gtgtaacctt cccatgccgt gtcctgtacc
    48301 acttcccagc cgtgctgctg cgcgtgttgc atggtcaggc gaacggtatc gtcatagttc
    48361 atatccgtga cgatgcactc ggcaccgagg ttcagaatgg cgtcaacgcg ttcctgagca
    48421 gaacctttcg gcatgtaaat caccgcattc tgtccgagtt gctgtgctgc ccacgccaca
    48481 ccgcgcccgt ggttgccgtc ggtggtggtc gcgaaagtca ttttttcgcc gatggcattt
    48541 ttcaggtgct caaatgacag cgtttcgata tcaagatgat atttttcaca caataattga
    48601 gcgatggcgt acgcaccgcc aagcattttg aaggcgttca gaccgaatcg ttttgattcg
    48661 tctttgacga gaattttctt cacaccaaaa aggtttgcga gatcgtccag cgcacaaagc
    48721 ggtgttgggc gataaccagc tattttctgg tggaactggc gcgccagttt ggcctggctt
    48781 tgcgaaaaga gcggtgatgt ttcgccgttg aaaaatttgt tatcggcgat atcaatcttc
    48841 aatgagaaaa cggacatatc ctatcctttt ggcctctgga aatagagggc cgaccagcag
    48901 atcggcccga ggggttattt cacgcgttct tgcgcttctt tcagcagttg ctcgagcagt
    48961 gcacctggtt tggcgtattt acgggacagg atcatcgcgg cgatgatata aggcttccag
    49021 ctggcttctt tgtaggtagc gatacggtat ttttcgaata cgccttcagt cacttcacct
    49081 tctttgcagg atacgccgct gatatcagct ggcaggcagt gcatgtacag ggcttcacca
    49141 tcacgggtca gttccatcat ctcttcagta caatgccagt ctttgtgttg cgcgttctgt
    49201 gccagacact gtttttccag tgcttttaag ccttcgtggt cgttcgcacg cagcaattca
    49261 gtacgctctt ccatcacttt gtaaggtgcc catgacttcg gataaacgat gtctgcgtct
    49321 ttgaaggctt cttccatgct ggtgacctga cggaagctac caccggaggc tttagcattg
    49381 tttttcgcca cttcaaccac atccgggatc aggtcgtagc cttccggatg ggccaaggtg
    49441 acatccatac cgaagcgagt catcagaccg atgatgcctt gtggtacaga gagcggtttg
    49501 ccatagcttg gagagtaggc ccaggtcatg gcgatttttt tacctttcag gttttccagt
    49561 gaaccaaagt gttcacgtaa ccacgcgagg tcagccattg actgagtcgg gtggtcaata
    49621 tcgcattgca ggttcactaa agccggacgc tgtggcagta caccctgctt gtaaccgtca
    49681 tcaagtgcag cgccaacttc acgcatatag gcgttgcctg cgcccagata catatcgtcg
    49741 cgaataccaa tagcgtcggc gcagaaggag atcatattgg cggtttcacg cacggtttcg
    49801 ccgtgagcga tttgtgattt gccttcgtcg agat
//
LOCUS       DAKISA010000019        48781 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.019, whole genome shotgun sequence.
ACCESSION   DAKISA010000019 DAKISA010000000
VERSION     DAKISA010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48781
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.019"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..515)
                     /locus_tag="OVW45_001135"
     CDS             complement(<1..515)
                     /locus_tag="OVW45_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HCV5525640.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPAPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHR"
     gene            complement(675..887)
                     /gene="rpmE"
                     /locus_tag="OVW45_001136"
     CDS             complement(675..887)
                     /gene="rpmE"
                     /locus_tag="OVW45_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCV5525641.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            1090..3288
                     /gene="priA"
                     /locus_tag="OVW45_001137"
     CDS             1090..3288
                     /gene="priA"
                     /locus_tag="OVW45_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCV5525642.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQTLRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3444..4469
                     /gene="cytR"
                     /locus_tag="OVW45_001138"
     CDS             3444..4469
                     /gene="cytR"
                     /locus_tag="OVW45_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCV5525643.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4561..5520
                     /gene="ftsN"
                     /locus_tag="OVW45_001139"
     CDS             4561..5520
                     /gene="ftsN"
                     /locus_tag="OVW45_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCV5525644.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5613..6143
                     /gene="hslV"
                     /locus_tag="OVW45_001140"
     CDS             5613..6143
                     /gene="hslV"
                     /locus_tag="OVW45_001140"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCV5525645.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            6153..7484
                     /gene="hslU"
                     /locus_tag="OVW45_001141"
     CDS             6153..7484
                     /gene="hslU"
                     /locus_tag="OVW45_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCV5525646.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7551..8477
                     /gene="menA"
                     /locus_tag="OVW45_001142"
     CDS             7551..8477
                     /gene="menA"
                     /locus_tag="OVW45_001142"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCV5525647.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLVLITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8570..9055
                     /gene="rraA"
                     /locus_tag="OVW45_001143"
     CDS             8570..9055
                     /gene="rraA"
                     /locus_tag="OVW45_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCV5525648.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(9140..9385)
                     /gene="zapB"
                     /locus_tag="OVW45_001144"
     CDS             complement(9140..9385)
                     /gene="zapB"
                     /locus_tag="OVW45_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCV5525649.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9811..10656
                     /gene="glpF"
                     /locus_tag="OVW45_001145"
     CDS             9811..10656
                     /gene="glpF"
                     /locus_tag="OVW45_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HCV5525650.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10679..12190
                     /gene="glpK"
                     /locus_tag="OVW45_001146"
     CDS             10679..12190
                     /gene="glpK"
                     /locus_tag="OVW45_001146"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCV5525651.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDVM"
     gene            12320..13330
                     /gene="glpX"
                     /locus_tag="OVW45_001147"
     CDS             12320..13330
                     /gene="glpX"
                     /locus_tag="OVW45_001147"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCV5525652.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13427..14173
                     /gene="fpr"
                     /locus_tag="OVW45_001148"
     CDS             13427..14173
                     /gene="fpr"
                     /locus_tag="OVW45_001148"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCV5525653.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLEE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(14178..14606)
                     /gene="uspD"
                     /locus_tag="OVW45_001149"
     CDS             complement(14178..14606)
                     /gene="uspD"
                     /locus_tag="OVW45_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCV5525654.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14633..14932)
                     /locus_tag="OVW45_001150"
     CDS             complement(14633..14932)
                     /locus_tag="OVW45_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCV5525655.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(15144..15584)
                     /locus_tag="OVW45_001151"
     CDS             complement(15144..15584)
                     /locus_tag="OVW45_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCV5525656.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15685..16284
                     /locus_tag="OVW45_001152"
     CDS             15685..16284
                     /locus_tag="OVW45_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCV5525657.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            16392..17159
                     /gene="tpiA"
                     /locus_tag="OVW45_001153"
     CDS             16392..17159
                     /gene="tpiA"
                     /locus_tag="OVW45_001153"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCV5525658.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(17214..17969)
                     /gene="cdh"
                     /locus_tag="OVW45_001154"
     CDS             complement(17214..17969)
                     /gene="cdh"
                     /locus_tag="OVW45_001154"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCV5525659.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(18076..19065)
                     /gene="sbp"
                     /locus_tag="OVW45_001155"
     CDS             complement(18076..19065)
                     /gene="sbp"
                     /locus_tag="OVW45_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCV5525660.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19385..20347)
                     /gene="pfkA"
                     /locus_tag="OVW45_001156"
     CDS             complement(19385..20347)
                     /gene="pfkA"
                     /locus_tag="OVW45_001156"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCV5525661.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20528..21430)
                     /gene="fieF"
                     /locus_tag="OVW45_001157"
     CDS             complement(20528..21430)
                     /gene="fieF"
                     /locus_tag="OVW45_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCV5525662.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21578..22078)
                     /gene="cpxP"
                     /locus_tag="OVW45_001158"
     CDS             complement(21578..22078)
                     /gene="cpxP"
                     /locus_tag="OVW45_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCV5525663.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            22228..22926
                     /gene="cpxR"
                     /locus_tag="OVW45_001159"
     CDS             22228..22926
                     /gene="cpxR"
                     /locus_tag="OVW45_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCV5525664.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22923..24296
                     /gene="cpxA"
                     /locus_tag="OVW45_001160"
     CDS             22923..24296
                     /gene="cpxA"
                     /locus_tag="OVW45_001160"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCV5525665.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(24402..25076)
                     /gene="yiiM"
                     /locus_tag="OVW45_001161"
     CDS             complement(24402..25076)
                     /gene="yiiM"
                     /locus_tag="OVW45_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCV5525666.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(25225..26208)
                     /gene="kdgT"
                     /locus_tag="OVW45_001162"
     CDS             complement(25225..26208)
                     /gene="kdgT"
                     /locus_tag="OVW45_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HCV5525667.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(26468..27088)
                     /gene="sodA"
                     /locus_tag="OVW45_001163"
     CDS             complement(26468..27088)
                     /gene="sodA"
                     /locus_tag="OVW45_001163"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCV5525668.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            27373..28407
                     /gene="rhaT"
                     /locus_tag="OVW45_001164"
     CDS             27373..28407
                     /gene="rhaT"
                     /locus_tag="OVW45_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HCV5525669.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNTGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(28404..29270)
                     /gene="rhaR"
                     /locus_tag="OVW45_001165"
     CDS             complement(28404..29270)
                     /gene="rhaR"
                     /locus_tag="OVW45_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCV5525670.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(29326..30162)
                     /gene="rhaS"
                     /locus_tag="OVW45_001166"
     CDS             complement(29326..30162)
                     /gene="rhaS"
                     /locus_tag="OVW45_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCV5525671.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRAHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            30450..31919
                     /gene="rhaB"
                     /locus_tag="OVW45_001167"
     CDS             30450..31919
                     /gene="rhaB"
                     /locus_tag="OVW45_001167"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCV5525672.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACREMAQPLPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQLHSTRQTKELCA"
     gene            31916..33175
                     /gene="rhaA"
                     /locus_tag="OVW45_001168"
     CDS             31916..33175
                     /gene="rhaA"
                     /locus_tag="OVW45_001168"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCV5525673.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLTPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            33417..34241
                     /gene="rhaD"
                     /locus_tag="OVW45_001169"
     CDS             33417..34241
                     /gene="rhaD"
                     /locus_tag="OVW45_001169"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCV5525674.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHGNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            34251..34565
                     /gene="rhaM"
                     /locus_tag="OVW45_001170"
     CDS             34251..34565
                     /gene="rhaM"
                     /locus_tag="OVW45_001170"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCV5525675.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            34866..35312
                     /locus_tag="OVW45_001171"
     CDS             34866..35312
                     /locus_tag="OVW45_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCV5525676.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            35323..36774
                     /locus_tag="OVW45_001172"
     CDS             35323..36774
                     /locus_tag="OVW45_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCV5525677.1"
                     /translation="MESSLCIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            36764..37834
                     /locus_tag="OVW45_001173"
     CDS             36764..37834
                     /locus_tag="OVW45_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCV5525678.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            37834..39582
                     /locus_tag="OVW45_001174"
     CDS             37834..39582
                     /locus_tag="OVW45_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HCV5525679.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNFQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGNEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELIAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAADIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(39632..40672)
                     /locus_tag="OVW45_001175"
     CDS             complement(39632..40672)
                     /locus_tag="OVW45_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HCV5525680.1"
                     /translation="MKRNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAP
                     EAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQNSLARYADWLKDFK
                     QGPTGKESTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNT
                     INEMDKYYTQENYKDDAFAKGKTLHQTFLKNLESFEPVAESYHAAIQEINDKRQLAEL
                     KNIEEREGKTFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADK
                     GGMNFSFINSADQYQLETKKYVRRVRDKVPYSDWDKEHLQDANTSWMVEDSFPRALRE
                     YNEMVDDYNSLR"
     gene            complement(40825..41658)
                     /gene="fdhD"
                     /locus_tag="OVW45_001176"
     CDS             complement(40825..41658)
                     /gene="fdhD"
                     /locus_tag="OVW45_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCV5525681.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            41852..44902
                     /gene="fdnG"
                     /locus_tag="OVW45_001177"
     CDS             41852..44902
                     /gene="fdnG"
                     /locus_tag="OVW45_001177"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:42437..42439,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCV5525682.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            44915..45817
                     /gene="fdoH"
                     /locus_tag="OVW45_001178"
     CDS             44915..45817
                     /gene="fdoH"
                     /locus_tag="OVW45_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCV5525683.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            45814..46449
                     /gene="fdoI"
                     /locus_tag="OVW45_001179"
     CDS             45814..46449
                     /gene="fdoI"
                     /locus_tag="OVW45_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCV5525684.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            46446..47375
                     /gene="fdhE"
                     /locus_tag="OVW45_001180"
     CDS             46446..47375
                     /gene="fdhE"
                     /locus_tag="OVW45_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCV5525685.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(47705..47947)
                     /locus_tag="OVW45_001181"
     CDS             complement(47705..47947)
                     /locus_tag="OVW45_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525686.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
BASE COUNT        11897 a        12659 c        12637 g        11588 t
ORIGIN      
        1 cgatgcggac ttaagcggag ttcttccggc agcgcctgcc agagtgcggc cagccggtga
       61 ccttcatcca gtttcgccac gccgccgcgc accagccaga gtgattcgct gcggctgtaa
      121 ccgtcctcac cgggaaacag gtgctcaaaa tacaggcttc tgccgccgtt atcactgagt
      181 atcagtgtgt tatcgcgcag ctgtaagcgg atatccgcag gcattttcca gccggggccg
      241 aggctcccca ccggcgcggg cgtttttgtc cggtaactgc tgtaggtgcg ggagaggatg
      301 aacggcagcg gggcgggcag ggcgatgtcg gtttcaccgg gaaggacctt cgcaccgagc
      361 agcggattga ccggatggcc ggacgtcacc ccgccggggc acaccgaaca ggccacgccg
      421 gtgggggcac cgatgcgcac cccggctgaa ccctggacaa tgctaccgcc atactgcgtc
      481 atgtcgccct gacgcgccgc cggttttccg ctcatcgtaa aatccttttg aaataaataa
      541 gaaccagaag cctagcgcca gtcaacaaca ggaaatgata atgagttaac accccggaat
      601 ttttcagaat ttataaaagg caaattacag gcacaaaaaa agcgccgtgc ggcgcttttt
      661 tcggaaatcc ggtcttattt gctgcccggg atgttgaaac gcttgttgaa gcggtcaaca
      721 cggccaccgg tagcaacatc acgctgtttg ccagtgaaga acgggtggca cttgctgcac
      781 acgtcgaggt tcaggtcatg accaacggtg gagcggattt tcattacgtt accgcaagag
      841 cagctagcag taatttcttc gtatttcggg tgaatatctt ttttcatggg aaaacctcgg
      901 tttaaggccg cgtcgctctt ccagccctaa cgccagacac cacgcgatgt taaaagtata
      961 gcttcaatac gatcatttcg tacgaaggcg cgaaatcata cagaaattaa ccagcgtatg
     1021 caaactgatc cgcactcttc tacggcaatg tgtatactaa cccaccgaat ttcaagtcag
     1081 gatgatgcta tgcccgttgc ccacgttgcc ttgcccgttc cgcttcctcg tacctttgac
     1141 tatctgctgc cagaaggcat ggcggttaaa gccgggtgtc gcgtgcgcgt gccgtttggc
     1201 aaacagcagg agcgcatcgg ggttgtggta tcagttagcg atgtcagtga gctgccgctt
     1261 aacgagctga aagcggtagt tgaagtgctg gacgttgagc cggtatttac tcactccgtc
     1321 tggcgattac tgctctgggc ggcagattac tatcatcatc cgattggcga tgtgctgttt
     1381 catgccttgc cgattttact gcgccagggg cggcctgcgg cgaacgcgcc gatgtggtac
     1441 tggtttgcca ctgaacaagg ccaggcggtg gatctgaaca gcctgaaacg ctcacccaag
     1501 caacaacagg cgctggcggc gttacggcaa gggaaaatct ggcgcgacca ggttgccacg
     1561 ctcgaattta atgatgccgc gttgcagacg ctgcgcaaaa aaggtctgtg tgatttagca
     1621 agtgaaacac cagagtttag cgactggcga acgaactatg ccgtttctgg tgagcggttg
     1681 cgattgaata ccgaacaggc caccgccgtt ggcgcaattc atagcgcggc agatactttt
     1741 tctgcctggc tgctggcggg cgttaccggt tccggtaaaa cggaggttta tctcagtgta
     1801 ctggaaaacg tgctcgctca gggcaaacag gcgctggtga tggtgccgga aatcggcctg
     1861 acaccgcaaa ctatcgcccg ttttcgtgaa cggtttaatg cgcccgtgga agttctgcat
     1921 tccggtctga acgacagcga gcgtctttcg gcgtggctga aagcgaaaaa tggcgaggcg
     1981 gcgattgtga tcggcacccg ctccgcgctg tttacgccgt ttaaaaatct cggcgtgatt
     2041 gtcattgacg aagagcacga cagttcctac aagcagcagg aaggctggcg ctatcatgcc
     2101 cgcgacctgg cggtgtatcg tgcgcatagc gagcaaatcc cgattattct tggctccgca
     2161 acgcccgcgc tggaaacgtt gtgcaacgtc cagcagaaaa aataccgcct gctgcgcctg
     2221 acgcgccggg cggggaatgc gcgtccggca attcaacacg tgctggattt aaaaggtcag
     2281 aaggtgcagg caggtctggc tccggcgtta atcacccgta tgcgccagca tttacaggcc
     2341 aacaatcagg ttatcctctt tcttaaccgc cgtggctttg cgcctgcgct actgtgccac
     2401 gactgcggct ggattgccga atgcccacgt tgcgatcact actacacgct gcatcaggcg
     2461 caacagcatt tgcgctgtca ccactgtgac agtcagcgtc cggtgccgcg ccagtgccct
     2521 tcctgcggtt ccacgcacct ggtacccgtg gggctgggca ccgaacagct tgaacagacg
     2581 cttgcgccgc tgttccctga tgtgcccatt tctcgtatcg accgcgatac caccagccgc
     2641 aaaggggcgc tggaacagca actggcggaa gtacatcgcg gcggtgcgag gattttgatc
     2701 ggcacacaaa tgctggcgaa aggtcaccac tttccggatg tgacgctggt ggcgttactg
     2761 gatgtggacg gcgcgctgtt ttctgccgat tttcgctcgg cagagcgttt cgctcagctt
     2821 tacacccagg tcgccggtcg tgccgggcgt gcgggtaaac agggcgaagt ggtgctgcaa
     2881 acgcaccatc cggaacatcc tctgttgcaa acgttgctct ataaaggcta cgacgccttt
     2941 gccgaacagg cgctggctga gcgacgcatg atgcagctgc cgccgtggac cagccatgtg
     3001 attgtgcgtg cggaagatca taacaatcag catgcgccgc tgttcctgca acaactgcgt
     3061 aatctgatcc tctccagccc gctggcagac gacaaattgt gggttctcgg tccggttccg
     3121 gcactggcac ctaaacgtgg cggtcgctgg cgctggcaga tattgttgca gcacccttcc
     3181 cgcgtgcgct tgcaacacat cattaacggt acgctggcac tcatcaatac aataccggat
     3241 tcccgtaagg tgaaatgggt gctggatgtt gatccgattg agggttaaac cgctcacgat
     3301 gcgaggcgga tcgaaaaatt caatattcat cacacttttc atgaaaattc tgtaaccgtt
     3361 ttcacgcgct atctgctaaa aatgttgccg atgtgaagta aacatggatg tagtacgcct
     3421 gacgtgccag gcgaggagtg agtgtgaaag cgaagaagca ggaaactgcc gcgaccatga
     3481 aagacgttgc cctcaaggca aaagtctcta cagcgactgt ctcccgagca ttaatgaatc
     3541 ccgataaagt ctcccaggcc acccgtaatc gggttgaaaa agcggcccgg gaagtgggtt
     3601 atttaccgca gcctatgggg cgcaacgtca agcgtaatga atcccgcacc attctggtga
     3661 ttgtcccgga tatctgcgat cccttcttta gcgaaattat tcgcggtatc gaagttacgg
     3721 cggcaaatca cggatatctg gtgctgattg gcgactgtgc gcatcaaaat cagcaggaaa
     3781 aaacctttat cgatttgatc atcaccaagc aaattgatgg catgttgctg ctgggttcaa
     3841 ggctgccgtt tgatgccagc attgaggaac agcgtaatct gccgccgatg gtgatggcga
     3901 acgaattcgc accggaactg gagctaccta cagttcatat cgacaatctg accgccgcat
     3961 ttgatgcagt aaattatcta tatgagcaag ggcataaacg gattggctgt atagccggtc
     4021 ccgaagagat gccgctgtgt cactaccgcc tgcaaggtta cgttcaggcg ctgcgtcgct
     4081 gcggcattat ggttgatccg caatacatcg cccgtggcga cttcaccttc gaagccggaa
     4141 gcaaagcgat gcagcagctg cttgatcttc cacaaccgcc tactgctgtc ttctgccata
     4201 gcgatgtgat ggcgctcggc gcactttctc aggcaaaacg ccaggggctg aaagtcccgg
     4261 aagacctttc cataatcggt tttgataaca tcgacctgac gcaattttgt gatccgccgc
     4321 tgacaaccat cgcgcagccg cgttacgaaa tcggtcggga agctatgctg ttattgcttg
     4381 atcaaatgca ggggcaacac gttggcagtg gctctcgttt aatggactgc gaacttatca
     4441 tccggggatc aacacgcgcg ttaccttaaa gtaaaccttt aagactgccg tgactggtca
     4501 aaggcccgcc gcttaagtaa catggcgggc tgacgaacga ataaatacag cgaaacgata
     4561 gtggcacaac gagattatgt acgccgcagc caaccggcac cttcgcggcg aaaaaagagc
     4621 acctcacgga aaaagcaacg aaatctgcct gcggtttctc ccgctatggt cgctattgct
     4681 gccgccgtgc ttgtgacctt tatcggtggt ctgtacttca ttacgcatca caagaaagaa
     4741 gagtccgaga cgctgcaaag ccagaaagtg accggtaacg gactaccacc aaaaccagaa
     4801 gaacgctggc gctacattaa agagctggaa agtcgccagc cgggtgtgcg tgcgcccaca
     4861 gaaccttctg ccggtggtga agtgaaaacg ccggagcaac tgacaccaga acaacgtcag
     4921 cttcttgaac aaatgcaggc tgatatgcgc cagcagccaa cgcagctggt tgaagttccg
     4981 tggaatgaac agacgccgga acagcgtcag caaacgctac aacgccagcg tcaggcgcag
     5041 caattagcgg agcagcaacg cctggctcag cagtcgcgta cgactgaaca aagctggcag
     5101 cagcagacgc gtacgtcgca agccgcgccg gtacaggcgc aaccgcgcca gtctaaaccg
     5161 gcttctactc agcagccgta ccaggatctg ctgcaaaccc ctgcgcacac gaccgcgcag
     5221 tcgaagccgc agcaagctgc gccggttgct cgtgccgctg acgcgccaaa accgacggcg
     5281 gagaaaaaag acgaacgccg ctggatggtg cagtgcggtt cgttcagagg cgcggaacag
     5341 gcagagacgg tacgtgctca gctggcgttc gaaggatttg actcgaaaat caccaccaac
     5401 aatggctgga atcgtgtggt cattggcccg gtgaaaggca aagagaacgc agacagcacc
     5461 ctcaatcggt tgaagatggc gggtcataca aactgcattc ggctcgccgc cgggggttga
     5521 aaccctcaaa atccccccca tctataattg cattatgccc cgtacttttg tacggggttt
     5581 gtactctgta ttcgtaacca aggggtcagc tcgtgacaac tatagtaagc gtacgccgta
     5641 acggccatgt ggtcatcgct ggtgatggtc aggccacgtt gggcaatacc gtaatgaaag
     5701 gcaacgtgaa aaaggtccgc cgtctgtaca acgacaaagt catcgcgggc tttgcgggcg
     5761 gtactgcgga tgcttttacg ctgttcgaac tgtttgaacg taaactggaa atgcatcagg
     5821 gccatctggt caaagccgcc gttgagctgg caaaagactg gcgtaccgat cgcatgctgc
     5881 gcaaacttga agcactgctg gcagtcgcgg atgaaactgc atcgcttatc atcaccggta
     5941 acggtgacgt ggtgcagcca gaaaacgatc ttattgctat cggctccggc ggcccttacg
     6001 cccaggctgc ggcgcgcgcg ctgttagaaa acactgaact tagcgcccgt gaaattgctg
     6061 aaaaggcgtt ggatattgca ggcgacattt gcatctatac caaccatttc cacaccatcg
     6121 aagaattaag ctacaaagcg taaggatctc ccatgtctga aatgacccca cgcgaaatcg
     6181 tcagcgaact ggataagcac atcatcggcc aggacaacgc caagcgttct gtggcgattg
     6241 ctctgcgtaa ccgctggcgt cgcatgcagc tcaacgaaga gctgcgccat gaagtgaccc
     6301 cgaaaaatat cctgatgatc ggcccgaccg gtgtcggtaa aactgaaatc gcccgtcgtc
     6361 tggctaagct ggcgaatgcg ccgttcatca aagttgaagc gaccaaattc accgaagtgg
     6421 gctatgtcgg taaggaagtg gattctatta ttcgcgatct gaccgatgcc gccgtgaaaa
     6481 tggtacgcgt ccaggctatc gagaaaaacc gttatcgcgc tgaagaactg gcagaagaac
     6541 gtattctcga cgtgctgatc ccacctgcta aaaacaactg gggacagacc gaacagcagc
     6601 aggaaccgtc cgctgctcgt caggcattcc gcaaaaaact gcgtgaaggc cagcttgatg
     6661 acaaagaaat cgagatcgat cttgccgcag caccgatggg cgttgaaatt atggctcctc
     6721 caggcatgga agagatgacc agccagctgc agtccatgtt ccagaacctg ggcggccaga
     6781 agcaaaaagc gcgtaagctg aaaatcaaag acgccatgaa gctgctgatt gaagaagaag
     6841 cggcgaaact ggtgaacccg gaagagctga agcaagacgc tatcgacgct gttgagcagc
     6901 acgggatcgt gtttatcgac gaaatcgaca aaatctgtaa gcgcggcgag tcttccggtc
     6961 cggatgtttc tcgtgaaggc gttcagcgtg acctgctgcc gctggtagaa ggttgcaccg
     7021 tttccaccaa acacgggatg gtcaaaactg accacattct gtttatcgct tctggcgcgt
     7081 tccagattgc gaaaccgtct gacctgatcc cggaactgca aggtcggctg ccaatccgcg
     7141 ttgaactgca ggcactgacc accagcgact tcgagcgtat tctgaccgag ccgaatgcct
     7201 ctatcaccgt gcagtacaaa gcactgatgg cgactgaagg cgtaaatatc gagtttaccg
     7261 actccggtat taagcgcatc gcggaagcgg catggcaggt gaacgaatct accgaaaaca
     7321 tcggtgctcg tcgtttacac actgttctgg agcgtttaat ggaagagatt tcctacgacg
     7381 ccagcgattt aagcggtcaa aatatcacta ttgacgcaga ttatgtgagc aaacatctgg
     7441 atgcgttggt ggcagatgaa gatctgagcc gttttatcct ataatcgcgt tcaatcattt
     7501 tcatcattgt ttgatggggc tgaaaggccc catttttatt ggcgcgtatt atgactgaac
     7561 aacaaattag ccgaactcag gcgtggctgg aaagtttacg acctaaaacc ctccccctcg
     7621 cctttgctgc aattatcgtc gggacggcgc tggcatggtg gcaaggtcac ttcgatccgc
     7681 tggtcgccct gctggtacta attaccgccg ggctattaca gatcctttct aacctcgcca
     7741 atgattacgg cgatgcggta aaaggcagcg ataaacctga ccgcattggg ccgctacgcg
     7801 gcatgcaaaa aggggtcatt acccagcaag agatgaaacg ggcgctcatt attaccgtcg
     7861 tgctcatctg tctctccggg ctggcactgg ttgcagtggc atgccatacg ctggccgatt
     7921 ttgtcggttt cctgattctt ggcgggttgt cgatcattgc cgctatcacc tacaccgtgg
     7981 gcaatcgtcc ttatggttat atcggtctgg gtgatatttc cgtactggtt ttctttggct
     8041 ggttgagcgt catggggagc tggtatttac aggctcatac attgattccg gcactgatcc
     8101 ttccggcgac cgcctgtggc ctgctggcaa cggcagtact gaacattaat aacctgcgtg
     8161 atatcaatag cgaccgcgaa aatggcaaaa acacgctggt ggtgcgctta ggtgaagtga
     8221 acgcgcgtcg ttatcatgcc tgcctgctga tgggctctct ggtatgtctg gcgctgttta
     8281 atctcttttc gctgcatagc ctgtggggct ggctgttcct gctggcggca ccattactgg
     8341 tgaagcaagc ccgttatgtg atgcgggaaa tggacccggt ggcgatgcga ccaatgctgg
     8401 aacgtactgt caagggagcg ttactgacta acctgctgtt tgttttaggg atattcctaa
     8461 gccagtgggc agcataactg acaaatatca attaacaatt gatgattttg ccaacagcct
     8521 acatagcgcg atatactgaa aattctcgca gcaactgaat gttaagccta tgaaatacga
     8581 tacttccgag ctttgtgaca tctatcaaga agatgttaac gtcgtggaac cgctgttctc
     8641 caactttggc ggacgggcgt cgtttggcgg acaaataatc acggtaaaat gtttcgagga
     8701 caacgggttg ctgtacgatc tgctcgaaca gaatggccgt ggtcgtgttc ttgtcgtcga
     8761 tggcggtggt tctgttcgtc gcgcactggt cgatgctgaa ctggcgcgtc tggcagtaca
     8821 aaatgaatgg gaaggtctgg ttatttacgg cgcggtgcgt caggtagatg acctagaaga
     8881 gttggatatc ggcatccagg cgatggcggc aattccggtt ggtgccgctg gcgaaggcat
     8941 tggcgaaagc gatgtccgcg tcaattttgg cggtgtaacc ttcttctccg gcgaccatct
     9001 ttatgccgac aataccggga ttattctttc agaagatccg ctggatattg aatgataaga
     9061 aaggcaccgc aaggtgcctt ttttctgcgt tacctgttgg cctacacagt aaagaaatta
     9121 cgcgaaagat gaagcgtaat cagacctctt ccatgcgacc cagcagggcc tgcagacgtt
     9181 cctgccagcc gttctgctgt tctttcagat ggttgttctc acgctccagc tcttcgcgct
     9241 gatgctgggc attttgaact tcctgcgaca gtgagttgtt tttttctttc agctcttcga
     9301 tttccatctg caacagagtg atggtatcaa tcgcctgctg tacttttgct tccagtttct
     9361 caaacacttc taatgacatt gtcatacctc tcctgaattg caaggcgttg atggataaaa
     9421 aatcctcgtc ccgattaccg gtgacgcctt aataaatacg agcgcacttt agttagctcc
     9481 gattgtatga agccgcgcca tcgctgtcca gcggcacgcc ttgcagatta cggtttgcca
     9541 cacttttcat ccttctcctg gtgacataat ccacatcaat cgaaaatgtt aataaatttg
     9601 ttgcgcgaat gatctaacaa acatgcatca tgtacaatca gatggaataa attgcgcgat
     9661 aacgctcatt ttatgacgaa gcacacacat tttaagttcg atatttctcg tttttgctcg
     9721 ttaacgataa gtttacagca tgcctacaag catcgtggag gtccgtgact ttcacgcata
     9781 caacaaacat taactcttca ggatccgatt atgagtcaaa catcaacctt gaaaggccag
     9841 tgcattgctg aattcctcgg taccgggttg ttgatttttt tcggtgtggg ttgcgttgca
     9901 gcactaaaag tcgctggtgc gtcttttggt cagtgggaaa tcagtgtcat ttggggactg
     9961 ggggtggcaa tggccatcta cctgaccgca ggggtttccg gcgcgcatct taatcccgct
    10021 gttaccattg cattgtggct gtttgcctgt ttcgacaagc gcaaagttat tccttttatc
    10081 gtttcacaag ttgccggcgc tttctgtgct gcggctttag tttacgggct ttactacaat
    10141 ttatttttcg acttcgagca gactcatcac attgttcgcg gcagtgttga aagtgttgat
    10201 ctggcaggca ctttctctac ttaccctaat cctcatatca attttgtgca ggctttcgca
    10261 gttgagatgg tgattaccgc tattctgatg gggctgatcc tggcgttaac ggacgatggc
    10321 aacggtgtac cacgcggccc tttggctccc ttgctgattg gtctactgat tgcggtcatt
    10381 ggcgcatcta tgggcccatt gacgggtttt gccatgaacc cagcgcgtga cttcggtccg
    10441 aaagtctttg cctggctggc gggctggggc aatgtcgcct ttaccggcgg cagagacatt
    10501 ccttacttcc tggtgccgct ttttggccct atcgttggcg cgattgtagg tgcatttgcc
    10561 taccgcaaac tgattggtcg ccatttgcct tgcgatatct gtgttgtgga agaaaaggag
    10621 accacaactc cttcagaaca aaaagcttcg ctgtaatatg actacgggac aattaaacat
    10681 gactgaaaaa aaatatatcg ttgcgctcga ccagggcacc accagctccc gcgcggtcgt
    10741 aatggatcac gatgccaata tcattagcgt gtcgcagcgc gaatttgagc aaatctaccc
    10801 aaaaccaggc tgggtagaac acgacccaat ggaaatctgg gccacccaaa gctccacgct
    10861 ggtagaagtg ctggcgaaag ccgatatcag ttccgatcaa attgcagcta tcggtattac
    10921 gaaccagcgt gaaaccacta ttgtctggga aaaagaaacc ggcaagccta tctataacgc
    10981 cattgtctgg cagtgccgtc gtaccgcaga aatctgcgag catttaaaac gtgacggttt
    11041 agaagattat atccgcagca ataccggtct ggtgattgac ccgtactttt ctggcaccaa
    11101 agtgaagtgg atcctcgacc atgtggaagg ctctcgcgag cgtgcgcgtc gtggtgaatt
    11161 gctgtttggt acggttgata cgtggcttat ctggaaaatg actcagggcc gtgtccatgt
    11221 gaccgattac accaacgcct ctcgtaccat gttgttcaac atccatacct tggactggga
    11281 cgacaaaatg ctggaagtgc tggatattcc gcgcgagatg ctgccagaag tgcgtcgttc
    11341 ttccgaagtg tacggtcaga ctaacattgg cggcaaaggc ggcacgcgta ttccaatctc
    11401 cgggatcgcc ggtgaccagc aggccgcgct gtttggtcag ttgtgcgtga aagaagggat
    11461 ggcgaagaac acctatggca ctggctgctt tatgctgatg aacaccggcg agaaagcggt
    11521 gaaatcagaa aacggcctgc tgaccaccat cgcctgcggc ccgactggcg aagtgaacta
    11581 tgcgctggaa ggtgcggtgt ttatggcagg cgcatccatt cagtggctgc gcgatgagat
    11641 gaagttgatt aacgacgcct acgattccga atatttcgcc accaaagtgc aaaacaccaa
    11701 tggtgtgtat gtggttccgg cctttaccgg gctgggtgcg ccgtactggg acccgtatgc
    11761 gcgcggggcg attttcggtc tgactcgtgg ggtgaacgct aaccacatta tacgcgcgac
    11821 gctggagtct attgcttatc agacgcgtga cgtgctggaa gcgatgcagg ccgactctgg
    11881 tatccgtctg cacgccctgc gcgtggatgg tggcgcagta gcaaacaatt tcctgatgca
    11941 gttccagtcc gatattctcg gcacccgcgt tgagcgcccg gaagttcgcg aagtgactgc
    12001 attgggcgcg gcctatcttg ctggtctggc ggtcggtttc tggcagaacc tcgacgagct
    12061 gcaagagaaa gcggtgattg agcgcgagtt ccgtccaggc atcgaaacca ctgagcgtaa
    12121 ttaccgttac gcaggctgga aaaaagcggt taaacgcgcg atggcgtggg aagaacacga
    12181 cgtaatgtaa atgccggatg aagcgtttat gccgcatccg gtagtcccga aacgtgcggg
    12241 ggcaaccccg cacacatcaa taatccctcc cttcccctgt gctacacttc gcgccattcc
    12301 ttactgctta gagtttgcta tgagacgaga acttgccatc gaattttccc gcgtcaccga
    12361 atctgcggcg ctggctggct acaaatggtt aggacgcggc gataaaaaca ccgcggacgg
    12421 cgcggcggta aacgccatgc gtattatgct caaccaggtc aatattgacg gcaccatcgt
    12481 cattggtgaa ggtgaaatcg acgaagcacc gatgctctac attggtgaaa aagtcggtac
    12541 tggtcgcggc gacgcggtag atattgctgt tgatccgatt gaaggcacgc gcatgacggc
    12601 gatgggccag gctaacgcgc tggcggtgct ggcagtaggc gataaaggct gcttcctcaa
    12661 tgcgccggat atgtatatgg agaagctgat cgtcggaccg ggagccaaag gcaccattga
    12721 tttgaacctg ccgctggcgg ataacctgcg caatgttgcg gcggcgctcg gcaaaccgtt
    12781 gagcgaactg acggtaacaa ttctggctaa accacgccac gatgccgtta tcgctgaaat
    12841 gcaacaactc ggcgtacgcg tatttgctat tccggatggc gacgttgcgg cctcaattct
    12901 cacctgtatg ccagacagcg aagttgacgt gctgtacggt attggtggcg cgccggaagg
    12961 cgtagtttct gcggcggtga tccgcgcatt agatggcgac atgaacggtc gtctgctggc
    13021 gcgtcatgac gtcaaaggcg acaacgaaga gaatcgtcgc attggcgagc aggagctggc
    13081 acgctgcaaa gcgatgggca tcgaagccgg taaagtattg cgcctgggcg atatggcgcg
    13141 cagcgataac gtcatcttct ctgccaccgg tattaccaaa ggcgatctgc tggaaggcat
    13201 tagccgcaaa ggcaatatcg cgactaccga aacgctgctg atccgcggca agtcacgcac
    13261 cattcgccgc attcagtcta tccactatct ggatcgcaag gacccggaaa tgcaggtgca
    13321 catcctctga ttgatttgat cgattgagcc ttccagtcct tcgggactgg aatttttttg
    13381 ttcggagaac gaagataagg caagtcaatc aaaacaggag aaaaacatgg ctgattgggt
    13441 aacaggcaaa gtcactaaag tgcagaactg gaccgacgcc ctgtttagtc tcaccgttca
    13501 cgcccccgtg cttccgttta ccgccgggca attcaccaag cttggccttg aaatcgacgg
    13561 cgaacgcgtc cagcgcgcct actcctatgt taactcgccc gataatcccg atctggagtt
    13621 ttacctggtc accgtccccg atggcaaatt aagcccacga ctggcggcac tgaaaccagg
    13681 cgatgaagtg caggtggtta gcgaagcagc tggcttcttt gttctggagg aagtaccaga
    13741 ttgcgaaacg ctatggatgc tggcaaccgg tacagcgatt ggcccttatt tatcgattct
    13801 gcaactaggc aaagatttag atcgcttcaa aaatctggtc ctggttcacg ccgcacgtta
    13861 tgccgccgac ttaagctatt tgccactgat gcaagaactg gaaaaacgct acgaaggcaa
    13921 actgcgcatt cagacggtgg tcagtcggga aacggcagcg gggtcgctca ccggacggat
    13981 accggcatta attgaaagtg gggaactgga aagcgcgatt ggcctgccga tgaataaaga
    14041 aaccagccat gtgatgctgt gcggcaatcc acagatggtg cgcgatacac aacagttgct
    14101 gaaagagacc cggcagatga cgaaacattt acgtcgccga ccgggccata tgacagcgga
    14161 gcattactgg taagcggtta cttatcgata aacggcacga tgagcaaatc cgcactcatc
    14221 ttattgatca tcccgcgata tgccggcatc aaacggttga taaatgagtg gtgatgacca
    14281 cagacaagga ggtcgcactg ctctttttgc ataatttcca gcagtgtttc cggcatttct
    14341 ccgcgttcaa tacgcagttt tgtcttcggc cattgaatat ttttcgtcag tttatacagc
    14401 ttgttatccg acttattctt caacaattga agaatatctt ctgttgcagg gaagtagata
    14461 cccggataca actcgcttaa gccatcatca atatgaatta acgtcaggtg agcgtcatta
    14521 tgtctggcga gctccagggc tttattcacc agtaaggcat cttcttcatt cccggaaatt
    14581 gccacgccaa tgtgtttata agccatgttt aactccttct aaagcaactc catcaagcta
    14641 acgaacgcag tgataactca aaaataatca tctcggcctg gcatgagaaa gtgaaggccg
    14701 catcaagctc aacttgcccc tccagatcct taatcttata ggccacgctg gccccttcat
    14761 ctgcagagga tgcagcggcg gcaagctctt tcagtttggt cataaaagac tctgcttctt
    14821 cgcgggtggc aaaaaagcgc gaaaacttac tggtacagtt gtcgttatca atcaccgtac
    14881 cgatatcaat cgcacatcct ttagtactgc atttatctac gacatctttc atagggcacc
    14941 tctgtatatt cgcctctctg tcgattacga cttaagaata atgcgccgtt aagggaagaa
    15001 caaacaagta atgatcttat ggattaccat tttttttgat attgcttaag gtgaagagtt
    15061 cagaaggatt ttccgggtat tgtcatttgc atcgtggaaa taacaatccc ggaaaaaaat
    15121 agcagtaaga gatatattaa aggttaatta cttgatttat tgtcggcttt atacttcaca
    15181 tcctgagtat ctttaccata tttattttcg ccttgtgtgc caacaaacgc accaagatcg
    15241 ataagcatca tcaccagaat caacgtcggg acaaaacgcc ccaccgccca ttgccagaca
    15301 cccggtaaaa tcgcccagtt acccgccagc agcatccacg ccacaatcat cagaaatgcc
    15361 catgcgccgg aacgcccgcg atcatgcaag cgcttaacag ttactgccgc tgttggccaa
    15421 agcaagcaca caaggcaaaa cgccgcggtc tgaatatcga gtaaattttt acccgccagt
    15481 gagaaaagca ccagcatgcc tgcgaaccac aggcctatcc aaatccagaa atcacggcgt
    15541 ccaatacgcc ctttaaatga gaataaccat tgctgtatgg tcatgtaagt tccttgatgg
    15601 ttgtcttttc caggattcta cccttttgac aagggcgaca gatatcgttt taatcggagc
    15661 cagtcataac aaaaggtact gtcaatgaag ccagggtgta cgctgttttt tctcttatgt
    15721 tctgcattaa ccgttacaac aacggcgcat gcacaaacac cagatacggc aacgaccgcg
    15781 ccttatctgc tggctggagc ccctactttc gatctctcca tcagccagtt tcgagaagac
    15841 tttaacagcc agaatcctag cctgccactg aacgaatttc gtgccatcga cagcagtccc
    15901 gacaaagcca atctcactcg tgctgcaagt aaaattaacg agaacttgta tgcttctaca
    15961 gcgctggagc gcggtacctt aaaaatcaaa agcattcaaa tgacctggct acccatccag
    16021 gggccagagc aaaaagcagc gaaagcgaaa gctcaggaat acatggcagc agtgatccgc
    16081 acactcaccc cattaatgac caaaacacaa agccagaaaa aactgcagtc gctactaacg
    16141 gcggggaaaa acaaacgtta ttacaccgag acagaaggtg cactgcgtta tgttgtcgcg
    16201 gacaacggcg aaaaggggct gaccttcgct gttgaaccga ttaagctggc gctatctgaa
    16261 tcgcttgaag gtttgaataa atgacaaaaa gcaaagcctt tgtgccgatg aatctctata
    16321 ctgtttcaca gacctgctgc cctgcggggc ggccatcttc ctttattcgc ttataagcgt
    16381 ggagaattaa aatgcgacat cctttagtga tgggtaactg gaaactgaac ggcagccgcc
    16441 acatggttca cgagctggtt tctaacctgc gtaaagagct ggcaggtgtt gctggctgtg
    16501 cggttgcaat cgcaccaccg gaaatgtaca tcgatatggc gaagcgcgaa gctgaaggca
    16561 gccacatcat gctgggtgcg caaaacgtgg acctgaacct gtccggcgca ttcaccggtg
    16621 aaacttctgc cgctatgctg aaagacatcg gcgctcagta catcatcatc ggccactctg
    16681 aacgtcgtac ttaccacaaa gagtctgacg aactgatcgc gaaaaaattc gcggtgctga
    16741 aagagcaggg cctgactccg gttctgtgca tcggtgaaac cgaagctgaa aacgaagcgg
    16801 gcaaaactga agaagtttgc gcacgtcaga tcgacgcggt actgaaaact cagggtgctg
    16861 cggcattcga aggtgcggtt atcgcttacg aacctgtatg ggcaatcggt actggcaaat
    16921 ctgcaactcc ggctcaggca caggctgttc acaaattcat ccgtgaccac atcgctaaag
    16981 ttgacgctaa catcgctgaa caagtgatca ttcagtacgg cggctctgta aacgcgtcta
    17041 acgctgcaga actgtttgct cagccagata tcgacggcgc gctggttggc ggtgcttctc
    17101 tgaaagctga cgctttcgca gtgatcgtta aagctgcaga agcggctaaa caggcttaag
    17161 tctgacaggt gccggatttc atatccggca cttactttcc ttaactcttc gccttaacgc
    17221 aaaatctcac actgatgatc ctgaatttcc tcggctgaag cacggttaag cgtcagtaga
    17281 tttcgttgtg tcgccagcaa tacaaatgag ttatcactct gccgtaccat cgccagcccg
    17341 tagcttccca tatgttcccg cgcctcaggt acttcttctg ccagcatcat aaatgggctg
    17401 cgttgtacca gttcgctttc cgttacccga cgcgccaggt attcatgccc gcgcaaacca
    17461 cctggcagtg gcaaccagcg gctgctgatg ttcgccagat tgttatccag ttgttcgcgc
    17521 acatcaggac gaatacaaga gatatgaata tgaaaatggt tttgcgtacg cccggtgcgg
    17581 gagttgatcg ccaaagaaac cgcgcgatcg ggaaccggct ggccgtattt tttgctcata
    17641 aaatcacgcg cctgccaggc caaccaaaag aagttcggcg ttgaaggatc ggtcaacaaa
    17701 ggactttcag taccgttaat acgatacgtt ggcatcaaca gatattgcag tggaccatta
    17761 agatctttta aaaccacgta tccggcattg ggtttgactt ccgcacatgg cgaaggattt
    17821 tgattttgct gctgattggg caaacattcc tcaaggacaa tcttacgtaa tgtatccgac
    17881 tcttcaccgg ttaatttcca gtaaccaata ccggcagcca caacggcgat aactatcatc
    17941 accaaaaaaa gaagacccgc ttttttcatc tttttttccc tgtacctcaa agagatgcaa
    18001 gggtaacgca aaatcgtgac aaataaaaaa cccggtcgcg agaaaccacc gggtttgata
    18061 attatcctgg ggaaatcagc gtttgctgat ctgatcgaac gtaccgccgt tagcaaaatg
    18121 ctctttttgc gctttcgtcc agccgccgaa ctcttcatca atggtgaata acttcagctt
    18181 tggaaacgca ttttcgtact ttttcgctac ttcagcgtcg cgcggacggt agtagttttt
    18241 cgcggcaatt tcctgacctt ctggcgagta gagatatttc aggtaggctt ccgccacctc
    18301 tttggtgcct tttttctcga ccactttatc gaccaccgac acggttggct ctgcgaggat
    18361 agactcactc ggcgtgacga tttcgaattt atctttcccc agttcattcg ctgccagcag
    18421 agcttcgttt tcccaggcga tcagtacatc gccaatcccg cgctcgacaa aggtgttagt
    18481 ggagccgcgc gcgccagaat ccagaacttc gacgttttta tacagtgccc gaacaaaatc
    18541 ctgtgctttt gcctgatcgt tgttgttgtg atgcagcgcg tagccccagg cagccaggta
    18601 gttccagcgt gcgccaccag agcttttcgg attaggcgtg atcaccgaaa cacccggttt
    18661 aatcagatcg ttccagtcat ggatctgctt cggattgccc ttacgcacca ggaaaacaat
    18721 ggtggaagtg tacggtgcgg agttatccgg cagacgtttg atccactctt tatcaatccg
    18781 cccgcgttcc gcaatcgcgt ccacgtcata ggccagagcc agcgtgacaa catcagcttc
    18841 aataccgttg attaccgacg tcgcttgttt acctgaacca ccgtgtgact gacgaatcac
    18901 cacgttatca ccagtttgct gtttccagtg ggcgctgaat gccttgttgt actgttcgta
    18961 caattcgcgc gttggatcat atgaaacgtt aagaagctga atatcctttg ccataacgct
    19021 ggttgccgcc agcaaaaatg ttaaccctac gccccacttg ttcatcgccc gactctctta
    19081 tgttgtgttg tgatgagcaa agcgtgccag aaggttaacc aaacattaaa gaataaaaaa
    19141 agattggcta taacttgcgg gtatatgttg agggattaaa aaggcggaag aaatccgcct
    19201 catattgctg acaaagtgcg ctttgtccat gccggatgcg gcgcgaacgc cttatccggc
    19261 ctacaaaagt ttgcaaattc aataaattgc agaattcatg taggcctgat aagcgaagcg
    19321 catcaggcat ttttgcttct gtcatcggtt tcaggctaaa ggaatctgcc tttttccgaa
    19381 atcattaata cagttttttc gcgcagtcca gccagtcacc tttgaacgga cgcttcatgt
    19441 tttcgatagc gtcgatgatg tcgtggtgaa ccagctgttc gttctggata cctacacaac
    19501 gaccgccgta acctgccagc agcagatcga tagcgtaagc gcccatacgg gaagccagaa
    19561 tacggtcgta aggcaccgga gaaccaccgc gctggatgtg gcccagcaca gttgcgcggg
    19621 tttcacgacc ggtttctttc tcgatgaaat gcgccagttc gtcaacatca cacatatgtt
    19681 cggtaatcgc cacgatcgcg tgttttttac ctttcgcgat acccgctttg atttcgttta
    19741 ccaggtcttc acggctgaat tcaacttccg gaaccacaac gaattcacag ccaccggcaa
    19801 tggccgcagc caacgtcaga tcgccacaat aacggcccat cacttccacc acggaaatac
    19861 gctggtgaga agaagaggtg tcacgcagac ggtcgatcgc ttctacaacg gtgctcagcg
    19921 cagtgaagaa accgatagtg tagtcagtgc ctttgatgtc gttgtcgata gtgcccggca
    19981 gaccgatgca cgggaagccc atttcggtca gacgcattgc acccatgtag gaaccgtcac
    20041 cgccgataac caccagcgcg tcgataccac gttttttcag gttttcgata gccacggcgc
    20101 ggatgttctc gtcgcggaat tccgggaagc gcgcagaacc gaggaacgta ccgccacggt
    20161 tgatcatgtc agaaacgctg taacggtcta gctgtaccat acggtcttca tacagaccca
    20221 gatagccgtc ataaatgccc attacttcca gaccttctgt cagcgcagaa cgaacaaccc
    20281 cgcgaattgc ggcgttcatg cctggcgcat caccgccgct tgtcaacaca ccgattttct
    20341 taatcatgac tacctctgaa ctttggaatg caaaataaaa tctgttgccg gaagtcttct
    20401 tgcacatcga agtgatccaa cgaatgtgca aatagtataa caatcacttc ctgctgaatt
    20461 gattcaggtc aggccaaatg gcggtatttt atacacaaaa tgcgggtctg gctctctttt
    20521 atactgatta tgaaagcata gaccgtttac cctccctggg tacgacggaa caggggtcct
    20581 gatggataat tacatccgat cccggaaaac gccgtaaaat agcctgctct acctgatccg
    20641 ccaccatatg tgcctgaacc aaaggcagag agtcttccat ttccaaatga atctgaataa
    20701 agcgggtcgg ccctgactgc cgcgtgcgaa gatcgtgagc gccgctaaca cccggccagg
    20761 aagtcacgat atcaataatt tcttgccgtt cctcatcagg caatgcgcga tccagtaatg
    20821 actgtaccgc ctcatatccc atgcgtaacg cgctatataa aatatagatg ccgattccca
    20881 atgcaaacag agcatcggcg cgatgccagc cgtaccagga caaccccagc gccagcagaa
    20941 ttgcgccgtt catcataaca tcagactggt aatgtagcat atcagcccgc accgcctggc
    21001 tttgcgtccg gcgcaccacc caccgctgaa acgagacaag gataatcgta caaattagcg
    21061 ccacaattgt cacgataacc ccgacgcctg gatctgtcat cggtgttgga gataccagat
    21121 gttgaatacc cgtcaaaaac aggaatagtg ccgaaccgga gataaacata ctttgcgcca
    21181 gcgccgcgag ggactcagct ttaccgtgac caaacgagtg attatcgtcg gcaggttgca
    21241 gggaatatcg caccaccagt aaattcgtca acgacgcgcc gatatccacc agcgaatcca
    21301 ccagcgcggc gagaatactc accgacccgg tataccacca tgcaaaaatt ttaatcagca
    21361 atagcagcga agccatcgcc gtcgcagcaa tcgccgcccg actgaccagc cgtccataag
    21421 attgattcat aaatactccc gctatcaact gacgctagta taacggaagc aaatcatctg
    21481 caatgcatta agcagcaggc aaattgagga taaaaaaacc cccacatcat gtgggggaag
    21541 acagggatgg tgtctatggc aaggaaaaca gggtttacta ctgggaacgt gagttgctac
    21601 tactcaatag cttcaacgat gaactttttt gccattgcgt cacgtcacgc aactgctcca
    21661 ttcgttgttg atgtttctcg tttaaaaccg cttgctgctc cggcgttaac aggcgataca
    21721 tttggttgcg gacttttgcc atctcaacct gacgagcaat ttgctcattc gccatttttt
    21781 ctgcctgtgc gcgcacagcg ttttcatcaa aattttctgc ggtgacaagg cgatgcattg
    21841 tctccagttc gctaacatta acaggaggct gttcgtgccg ggcctgttgc ataagatctc
    21901 gcatctgctg acgctgatgt tcggttaaac ttatgccgtc gaacatatgg ctctgcgtac
    21961 tgcgctgcgt aagttcttca cccggatgcc agttatcgcc tgaaccgact tcagcagcgt
    22021 ggcttaatga actgactgcc agcgttgagg ccatgacggc agcggtaact atgcgcatca
    22081 tttgctccca aaatctttct gtcgcgattc aacgatagag agtttacgat tcaggctgca
    22141 aacatgcgtc agggggtgta aaacaacgta aagtcatgga ttagcgacgt ctgatgacgt
    22201 aatttctgcc tcggaggtat ttaaacaatg aataaaatcc tgttagttga tgatgaccga
    22261 gagctgactt ccctattaaa ggagctgctc gagatggaag gcttcaacgt gattgttgcc
    22321 cacgatgggg aacaggcgct tgatcttctg gacgacagca ttgatttact tttgcttgac
    22381 gtaatgatgc cgaagaaaaa tggtatcgac acattaaaag cacttcgcca gacacaccag
    22441 acgcctgtca ttatgttgac ggcgcgcggc agtgaacttg atcgcgttct cggccttgag
    22501 ctgggcgcag atgactatct cccgaaaccg tttaatgatc gtgagctggt ggcacgtatt
    22561 cgcgcgatcc tgcgccgttc gcactggagc gagcaacagc aaaacaacga caacggttca
    22621 ccgacactgg aagttgatgc cttagtgctg aatccaggcc gtcaggaagc cagcttcgac
    22681 gggcaaacgc tggagttaac cggtactgag tttaccctgc tctatttgct ggcacagcat
    22741 ctgggtcagg tggtttcccg tgaacattta agccaggaag tgttgggcaa acgcctgacg
    22801 cctttcgacc gcgctattga tatgcacatt tccaacctgc gtcgtaaact gccggatcgt
    22861 aaagatggtc acccgtggtt taaaaccttg cgtggtcgcg gctatctgat ggtttctgct
    22921 tcatgatagg cagcttaacc gcgcgcatct tcgccatctt ctggctgacg ctggcgctgg
    22981 tgttgatgtt ggttttgatg ttacccaagc tcgattcacg ccagatgacc gagcttctgg
    23041 atagcgaaca gcgtcagggg ctgatgattg agcagcatgt cgaagcggag ctggcgaacg
    23101 atccgcccaa cgatttaatg tggtggcggc gtctgttccg ggcgattgat aagtgggcac
    23161 cgccaggaca gcgtttgtta ttggtgacca ccgaaggccg cgtgatcggc gctgaacgca
    23221 gcgaaatgca gatcattcgt aactttattg gtcaggccga taacgccgat catccgcaga
    23281 agaaaaagta tggccgcgtg gaactggtcg gtccgttctc cgtgcgtgat ggcgaagata
    23341 attaccaact ttatctgatt cgtccggcca gcagttctca atccgatttc attaacttac
    23401 tgtttgaccg cccgctatta ctgctgattg tcaccatgtt ggtcagtacg ccgctgctgt
    23461 tgtggttggc ctggagtctg gcaaaaccgg cgcgtaagct gaaaaacgct gccgatgaag
    23521 ttgcccaggg aaacttacgc cagcacccgg aactggaagc ggggccacag gaattccttg
    23581 ccgcaggtgc cagttttaac cagatggtca ccgcgctgga gcgcatgatg acctctcagc
    23641 agcgtctgct ttctgatatc tctcacgagc tgcgcacccc gctgacgcgt ctgcaactgg
    23701 gtacggcgtt actgcgccgt cgtagcggtg aaagcaagga actcgagcgt attgaaaccg
    23761 aagcgcaacg tctggacagc atgatcaacg atctgttggt gatgtcacgt aatcagcaaa
    23821 aaaacgcgct ggttagcgaa accatcaaag ccaaccagtt gtggagtgaa gtgctggata
    23881 acgcggcgtt cgaagccgag caaatgggca agtcgttgac agttaacttc ccgcctgggc
    23941 cgtggccgct gtacggcaat ccgaacgccc tggaaagtgc gctggaaaac attgttcgta
    24001 atgctctgcg ttattcccat acgaagattg aagtgggctt tgcggtagat aaagacggta
    24061 tcaccattac ggtggacgac gatggtcctg gcgttagccc ggaagatcgc gaacagattt
    24121 tccgtccgtt ctatcggacc gatgaagcac gcgatcgtga atctggcggt acaggattgg
    24181 ggctggcgat tgttgaaacc gccattcagc agcatcgtgg ctgggtgaag gcagaagaca
    24241 gcccgctggg cggtttacgg ctggtgattt ggttgccgct gtataagcgg agttaaactc
    24301 cgcatttgta ggcaggataa ggcgtttacg ccgcatccgg catttgagca ggatgcctga
    24361 tgcgacgctg atagcgtctt atcaggccta cactccgggg gttattttcc ccacaaccgg
    24421 cgggaaaaat cttcgatctt gccgctcagt cggcgctttt gcatcgtccg cgtccagctt
    24481 ttcgataacc cggcggcgga gagtaaacgg tgatactgat cgtcatcaaa cggcatatgc
    24541 catgcgatgg cggcagcctc ctgcacggtc acatcgctga cacgggaaac caactccagc
    24601 ggcgcgtccg cagacacttt ccctggcgct atcacgctat acaaccaccc caccttacct
    24661 gtgttttgca tcaactgcgc tatatcgctg atatcaaaat ggtaattgag tttatagcaa
    24721 ggcgagcgcg gctggctgac ctgaattaat gcctctcccc agcggaaaat atcgcccata
    24781 tgaacattac tttccgtgag accgtcggtt gagagatttt cgccaaacgc aggcgcaaca
    24841 aacaactccg cctgttccgg aaattcccgc acccagtaga gatagtgctc acgaggataa
    24901 tgacacagcg ctctgtctgg cccgccgtga acctttttct ccgcctgctc gtcaccttcc
    24961 agccccagct ccgtcagcat caactcacca tcaacctgga ttttggcaat tgcgctgggt
    25021 ttgccttcgg gataagcctg aatcttgcct gtgtatacat caaccggata tcgcatctgt
    25081 tccctctcca cgagaaattt ccccattaga ccatatttat tttatgtcag gcgtgctgtt
    25141 ttcgcggtaa gtatccaggc aggaactcaa tagcaaaccg gacgaacgtt agttcatccg
    25201 gctgtgggga ttaaggaagc aggtttattt caccgtacct aaaatttcga ttttcgctgc
    25261 tctggctttg actttacgtg accagataga ggtaagaatt ggcaccagaa tcgaagtcac
    25321 aatgaccgcc gtcgctacca gcgaagttgc tgccggagcc atcggtttaa acgcaggcac
    25381 catttctgca atcagcacag gtgtcgctac cgcggcccct gcggaactgg aagcggcaat
    25441 tccggcagtg ccatcgccac cgccaatcaa tttatcggcg ataatcaacg gaataccggt
    25501 cacgataatt actgccacac ccaacaggat ccccagcaaa ccagtctggg caatcacagt
    25561 caaatcaatg gtattgccca gcgcgaaggc aaagaatgga atcagcgttt gcactgcttt
    25621 gctgaaaaat tctcgtaatt cagggtcaag attccccagg gcaaagccca ccaggaacgg
    25681 taatactgcg ccgacgaaaa catgcggttc aaacgaggca atcccggcag tgcccagaat
    25741 aatcatcgtc atgagcggcc cggactccaa cgacatcaac acaaatgccc cagcttcttc
    25801 tttcgtaccg tactgctgca tgatggaagc gtaaagtccg ccgttggtca tatccatcgc
    25861 cgccaccagc gccagcgttg aaagtccggc aaagaatcca acttcaacac catgttccgg
    25921 aataatgcgc gaggcaattg ccgcaaccac ccacgcgacg gcaattttag ttaccaccag
    25981 cgtaccggat ttacgcagta ccgttcccgt cgcgcttaat tttattgacg cccccatgca
    26041 aaaaaaccac accgccagaa tgggcaccgt accggtaatc atcccgttgg tgaatgatcc
    26101 aaaatatttc cccgcccccg gcgagaaggt gtggcacagt gcgccaagga atagcgggac
    26161 gagcatcatc ccccccggga ttttctcaat cgagcgtttt atctgcattt ccatcacctg
    26221 tcggcatatt tttaagggct gatgcgtaat tcctcaatgg agcatcagtg ttgatggaaa
    26281 cgaagataga cattcattta atcaataaaa gtgatcaaaa ccacatcaat tgaaacgctg
    26341 ttttattttt atcggatcat tgtttttatt ttgaaattga tcacaaaaaa accaccgccg
    26401 ttggcgatgg ttcatttttt aagctgatat gcggcctata cgcctcattg cagcaggcgg
    26461 caaatgatta ttttttcgcc gcaaaacgtg ccgctgcttc gtcccagttc accacgttcc
    26521 agaactcttt aatgtagtcc ggacggcggt tctggaattt caggtagtaa gcatgttccc
    26581 acacatccag gcccagaatc gggaagccgg aagcgccaga aatagcttca cccatcagcg
    26641 gagaatcctg gttagcagta gaaaccaccg ccagtttatc gcctttcagc accagccatg
    26701 cccagccgga accaaagcgg gaagctgccg ctttttcaaa ttctgctttg aagttatcaa
    26761 cggagccgaa gtcacgttcg atagccgctt tcaggtcacc ctgcagggtg gtgccttttt
    26821 tcagaccttt ccagaacagg ctgtggttag cgtgaccgcc agcgttgttg cgcagtacgg
    26881 ttttcttgtc tgctggcagc tggtccagtt tggtgatcag ctcttcaacc ggcaggttgg
    26941 caaattctgg caggctttcc agcgccgcgt tggcgttgtt tacgtaggtc tgatggtgtt
    27001 tggtgtggtg gatttccatg gtctgcttat cgaagtgcgg ttccagggca tcgtaagcat
    27061 acggcaggga tggcagggta tagctcatat tcatctccag tattgtcggg cggccgattg
    27121 ttaatgccgc gtaagcagtt ggttcattat agttaattaa atgatattga aaatgattat
    27181 caatgccgta cttttcgtaa gggtatggtt ttgcaggaaa atgcccgaga tgtgaagcaa
    27241 atcacccact taatgccgtg attgccagta aatcgacaac ggcggcaaca ggcgaaaggt
    27301 taatcgacag cacgattttt acactcatct cgtcggagat gtgaagcgac gaaaaatgat
    27361 gaggataaga agatgagtaa cgcgattacg atggggatat tttggcattt gatcggcgcg
    27421 gccagtgcag cctgttttta cgctccgttc aaaaaagtaa aaaaatggtc atgggaaacc
    27481 atgtggtcag tcggtgggat tgtttcgtgg attattctgc cgtgggccat cagcgccctg
    27541 ttactaccgg atttctgggc gtattacagc tcgtttagtc tctctacgct gctgcctgtt
    27601 tttctgttcg gcgctatgtg ggggatcggt aatatcaact atggcctgac catgcgttat
    27661 ctcggcatgt cgatgggaat tggcatcgcc attggcatta cgttgattgt cggtacgctg
    27721 atgacgccaa ttatcaacgg caatttcgat gtgctgatta acaccgaagg cggacgcatg
    27781 acgttgctcg gcgttctggt ggcgctgatt ggcgtaggga ttgtaactcg agccgggcag
    27841 ttgaaagagc gcaagatggg cattaaagcc gaagagttca atctgaaaaa agggctggtg
    27901 ctggcggtga tgtgcggcat tttctctgcc gggatgtcct ttgcgatgaa cgccgcaaaa
    27961 ccgatgcatg aagccgctgc cgcacttggc gtcgatccac tgtatgtcgc tctgccaagc
    28021 tatgttgtca tcatgggcgg cggcgcgatc attaacctcg gtttctgttt tattcgtctg
    28081 gcaaaagtga aggatttgtc gctaaaagcc gacttctcgc tggcaaaacc gctgatcatt
    28141 cacaatgtgt tactctcggc actgggcggg ttgatgtggt atctgcaatt ctttttctat
    28201 gcctggggcc acgcccgcat tccggcgcag tatgactaca tcagttggat gctgcatatg
    28261 agtttctatg tattgtgcgg cggtatcgtc gggctggtgc tgaaagagtg gaacaataca
    28321 ggacgccgtc cggtaacggt gttgagcctc ggttgtgtgg tgattattgt cgccgctaac
    28381 atcgtcggca tcggcatggc gaattaatct ttctgcgaat tgagatgacg ccactggctg
    28441 ggcgtcatcc cggtttcccg ggtaaacacc accgaaaaat agttactatc ttcaaagcca
    28501 cattcggtcg aaatatcact gattaacagg cggctatgct ggagaagata ttgcgcatga
    28561 cacactctga cctgtcgcag atattgattg atggtcattc cagtctgctg gcgaaattgc
    28621 tgacgcaaaa cgcgctcact gcacgatgcc tcatcacaaa atttatccag cgcaaaggga
    28681 cttttcaggc tagccgccaa ccgggtaatc agcttatcca gcaacgtttc gctggatgtt
    28741 ggcggcaacg aatcactggt gtaacgatgg cgattcagca acatcaccaa ctgcccgaac
    28801 agcaactcag ccatttcgtt agcaaacgac acatgctgac tactttcatg ctcaagctga
    28861 ccgataacct gccgcgcctg cgccatcccc acgctaccta agcgccagtg tggttgccct
    28921 gcgctggcgc taaatcccgg aatcgctccc tgccagtcaa gattcagctt cagccgctcc
    28981 gggcaataaa taatattctg caaaaccaga tcgttaacgg aagcgtagga gtgtttatcg
    29041 tcagcatgaa tataaaagag atcgccacgg gtaatgcgat aaggacgatc gttgagtaca
    29101 tgcagaccat taccgcgcca gacaatcacc agctcacaaa aatcatgtgt atgttcggca
    29161 aagacatctt gcggataacg gtcagccaca gcgactgcct gctggtcgct ggcaaaaaaa
    29221 tcatctttga gaagttttaa ctgatgcgcc accgtggcta cctcggccag agaacgaagt
    29281 tgattattcg caatatggcg tacaaatacg ttgagaagat tcgcgttatt gcagaaagcc
    29341 atcccgtccc tggcgaatat cacgcggtga ccagttaaac tctcggcgaa aaagcgtcga
    29401 aaagtggtta ctgtcgctga atccacagcg ataggcgatg tcagtaacgc tggcctcgct
    29461 gtggcgtagc agatgtcggg ctttcatcag tcgcaggcgg ttcaggtatc gctgaggcgt
    29521 cagtcccgtt tgctgcttaa gctgccgatg tagcgtacgc agtgaaagag aaaattgatc
    29581 cgccacggca tcccaattca cctcatcggc aaaatggtcc tccagccagg ccagaagcaa
    29641 gttgagacgt gatgcgctgt tttccaggtt ctcctgcaaa ctgcttttac gcagcaagag
    29701 cagtaattgc ataaacaaga tctcgcgact ggcggtcgag ggtaaatcat tttccccttc
    29761 ctgctgttcc atctgtgcaa ccagctgtcg cacctgctgc aatacgctgt ggttaacgcg
    29821 ccagtgagac ggatactgcc catccagctc ttgtggcagc aactgattca gcccggcgag
    29881 aaactgaaat cgatccggcg agcgatacag cacattggtc agacacagat tatcggtatg
    29941 ttcatacaga tgccgatcat gagcgcgtac gaaacagacc gtgccaccgg tgatggtata
    30001 gggctgccca ttaaacacat gaatacccgt gccatgttcg acaatcacaa tttcatgaaa
    30061 atcatgatga tgttcaggaa aatccgcctg cgggagccgg ggttctatcg ccacggacgc
    30121 gttaccagac ggaaaaaaat ccacactatg taatacggtc atactggcct cctgatgtcg
    30181 tcaacacggc gaaatagtaa tcacgaggtc aggttcttac cttaaatttt cgacggaaaa
    30241 ccacgtaaaa aacgtcgatt tttcaagata cagcgtgaat tttcaggaaa tgcggtgagc
    30301 atcacatcac cacaatgaag caaattgtga acatcatcac gttcatcttt ccctggttgc
    30361 caatggccca ttttcctgtc agtaacgaga aggtcacgaa ttcaggcgct ttttagactg
    30421 gtcgtaatga aattcagcag gatcacatta tgacctttcg caattgtgtc gccgtcgatc
    30481 tcggcgcatc cagtgggcgc gtgatgctgg cgcgttacga gcgtgaatgc cgcagcctga
    30541 cgctgcgcga aatccatcgt tttaacaatg gcctgcatag ccagaacggt tatgtcacct
    30601 ggaatgtgga tagcctggaa agtgccattc gccttggatt aaacaaggtg tgcgaggaag
    30661 ggattcgtat cgatagcatt gggattgata cctggggcgt ggactttgtg ctgctcgacc
    30721 aacagggtca gcgtgtgggc ctgcccgttg cttatcgcga tagccgctcc aatggcctaa
    30781 tggcgcaggc acagcaacaa ctcggcaaac gcgatattta tcaacgtagc ggcatccagt
    30841 ttctgccctt caatacgctt tatcaactgc gtgcgctgac ggagcaacaa cctgaactta
    30901 ttccacacat tgctcacgct ctgctgatgc cggattactt cagctatcgc ctgaccggca
    30961 agatgaactg ggagtacacc aacgccacga ccacgcaact ggtcaatatc aatagcgacg
    31021 actgggacga gtcgctactg gcgtggagcg gggctaacaa agcctggttt ggtcgcccga
    31081 cgcatccggg taatgtcatt gggcactgga tttgcccgca gggtaatgag attccggtgg
    31141 tcgccgttgc cagccatgat accgccagcg cggttatcgc ctcgccgtta aacggttcac
    31201 gcgctgctta tctctcttct ggcacctggt cattgatggg cttcgaaagc cagacgccat
    31261 ttaccaatga cacggcgctg gcagccaaca taaccaatga aggcggggcg gaaggtcgct
    31321 atcgggtgct gaaaaatatt atgggcttat ggctgcttca gcgagtgctt caggagcggc
    31381 aaatcaacga tctcccggcg cttatcgccg cgacacaggc acttccggcc tgccgcttca
    31441 tcatcaatcc caatgacgat cgctttatta atcctgaggc gatgtgcagc gaaattcagg
    31501 ctgcgtgtcg ggaaatggcg caaccgctcc cggaaagtga tgctgaactg gcgcgctgca
    31561 ttttcgacag tctggcgctg ctgtatgccg atgtgttgca tgagctggcg cagctacgcg
    31621 gtgaagattt ctcgcaactg catattgtcg gcggcggctg ccagaacacg ctgctcaacc
    31681 agctatgtgc cgatgcctgc ggtattcggg tgatcgccgg gcctgttgaa gcctcgacgc
    31741 tcggcaatat cggcatccag ttaatgacgc tggatgaact caacaatgtg gatgatttcc
    31801 gtcaggtcgt cagcaccacc gcgaatctga ccacctttac ccctaatcct gacagtgaaa
    31861 ttgcccacta tgtggcgcag cttcactcga cacgacagac aaaggagctt tgcgcatgac
    31921 cactcaactg gaacaggcct gggagctggc gaaacagcgt ttcgcggcgg tggggattga
    31981 tgtcgaggag gcgctgcgcc aacttgatcg tttacccgtt tcaatgcact gctggcaggg
    32041 cgatgatgtt tccggttttg aaaacccgga aggttcgctg accggtggga ttcaggctac
    32101 tggtaattat ccgggcaaag cgcgtaatgc cagtgagcta cgagccgatc tggaacaggc
    32161 tatgcggctg attccggggc cgaaacggct taatttacat gccatctatc tggaatcaga
    32221 cacgccagtc tcgcgcgacc agatcaaacc tgagcacttc aaaaactggg ttgaatgggc
    32281 gaaagccaat cagctcggtc tggattttaa cccctcctgt ttctcgcatc cgctaagcgc
    32341 cgatggcttt acgctctcgc atcccgacga cagcattcgc cagttctgga ttgatcactg
    32401 caaagccagc cgtcgcgttt cggcctattt tggcgagcaa ctcggcacac catcggtgat
    32461 gaacatctgg atcccggatg gtatgaaaga tatcaccgtt gaccgtctca ccccgcgtca
    32521 gcgtctgctg gcagcactgg atgaggtgat cagcgagaag ctggatccgg cgcaccatat
    32581 cgacgccgtt gagagcaaat tgtttggcat tggcgcagag agctacacgg ttggctccaa
    32641 tgagttttac atggggtatg ccaccagccg ccagactgcg ctgtgcctgg acgccgggca
    32701 tttccacccg actgaagtga tttccgacaa gatttccgcc gccatgctgt atgtaccgca
    32761 gttgctgctg cacgtcagcc gtccggttcg ctgggacagc gatcacgtgg tgctgctgga
    32821 tgatgaaacc caggcgattg ccagtgagat tgttcgtcac gatctgttcg accgggtgca
    32881 tatcggcctc gacttctttg atgcctctat caaccgcatt gccgcgtggg tcattggtac
    32941 acgcaatatg aaaaaagccc tgctgcgtgc gttgctggaa cctaccgctg agctgcgcaa
    33001 gctggaagcg gcgggcgatt acactgcgcg tctggcgcta ctggaagaac agaaatcgtt
    33061 gccgtggcag gcggtctggg aaatgtattg ccaacgtcac gatacgccag caggtagcga
    33121 atggctggag agcgtgcggg cttatgagaa agcgattttg agccagcgtg ggtaaacact
    33181 gccggatgcg gcgcgagcgc cttatccggc ctacaggtcg aatgaccatg cgtgctgcca
    33241 ccctcacccc ggccctctcc ctgaaagggc gagggagaaa aacatagcct cgtgcggcaa
    33301 cggagctgaa caccgctccg gacagtcccc tcgcccctac ggggagaggg ttagggtgag
    33361 gggaacaagc cgcaccatgt ctaaacaagg caacctaatt tacaggaaca cagaacatgc
    33421 aaaacattac ccagtcctgg tttgtccagg gaatgatcaa agccaccacc gatgcctggc
    33481 tgaaaggctg ggacgagcgc aatggtggta acctgacgct gcgcctggat gacgccgata
    33541 tcgcgcctta tcacggcaac tttcatgcac aaccgcgcta tattcccctc agccaaccca
    33601 tgcctttact ggcaaatacg ccgtttattg tcaccggctc cggcaaattc ttccgtaacg
    33661 tccagcttga tcctgcagct aacttaggcg tcgtaaaagt cgacagcgac ggcgcgggct
    33721 accatattct ctgggggtta accaacgaag ccgttccaac ttccgaactt ccggctcact
    33781 tcctttccca ctgcgagcgc attaaagcca ccaacggtaa agatcgggtg atcatgcact
    33841 gccacgccac caacctgatc gccctcacct atgtacttga aaacgacacc gcggtcttta
    33901 ctcgccaact gtgggaaggc agcaccgagt gtctggtggt attcccggat ggcgttggca
    33961 ttctgccgtg gatggtgccc ggcacggacg aaatcggcca ggcgaccgca caagagatgc
    34021 aaaaacattc gctggtgttg tggcccttcc acggcgtctt cggcagcggg tcgacgttgg
    34081 atgaaacatt cggtttaatc gacaccgcag aaaaatcagc acaagtatta gtgaaggttt
    34141 attcgatggg cggcatgaaa cagaccatca gccgtgaaga gttgatagcg ctcggcaagc
    34201 gcttcggcgt tacaccacta gctagtgcgc tggcgctgta aggagcaaac atgatccgca
    34261 aagcctttgt catgcaggtt aaccccgacg cccacgaaga gtatcagcgt cggcataacc
    34321 ccatctggcc ggaactggaa gcagtgttga aatctcacgg tgcgcataac tacgccattt
    34381 acctcgacaa agcgcgtaat ctgctgtttg ccacggtaga gattgaatct gaagaacggt
    34441 ggaatgcggt agccagcact gatgtttgcc aacgttggtg gaaatatatg accgatgtta
    34501 tgcccgctaa cccggataac agcccggtga gtagcgagct gcaagaagtg ttttacctgc
    34561 cgtaattgcc caatgtgcca gagatgcctc cggcacaata ttttaaattt cgatttgaaa
    34621 aattaaacat attacatctc ccggtaagtt atatttccct gatacattgt gagtaaatca
    34681 caaaaataat gaataaccca ttaatgattc atgtggttta tttaaataac cattatgtgc
    34741 attactccgc aaatctgacc tttcactctg tctcaaattg tcattgttta ccgacatatc
    34801 gcctctcatc acttaatcgc tccaccatta caattgatta aaatatattt tatggagtcc
    34861 gattaatggc agctcttact gcaagctgta ttgacctgaa tattcagggc aatggcgctt
    34921 attccgttct gaagcagttg gcgacaatag cgttacaaaa cggttttatc accgactcac
    34981 accagttcct gcaaaccctg ctgctgcgcg aaaaaatgca ctctactgga tttggttccg
    35041 gtgtcgccgt gccgcacggt aaaagcgcct gcgttaaaca accgtttgta ttattcgccc
    35101 gcaaagcgca ggctattgac tggaaagcca gcgatggcga agacgtcaat tgctggatct
    35161 gcctcggcgt gccgcaaagc ggcgaagagg atcaggtcaa aatcatcggc acactgtgtc
    35221 gcaaaattat ccaccaggat tttattcatc aactgcaaca gggcgacgcc gaccaggtgc
    35281 ttgccttgtt aaatcaaacc ctcagctcat aaggaagtgg cgatggagtc atccttatgt
    35341 attgtcgcga tcaccaactg ccccgccggg atcgctcaca cctacatggt ggcggaagcc
    35401 ctggaacaga aagcccgttc tctcggccat accataaaag tggaaactca agggtccagt
    35461 ggcgttgaaa accgcttatc cagcgaagag attgccgctg ccgattacgt cattctcgct
    35521 accgggcgtg gcctgagcgg tgatgatcgc gcgcggtttg ccgggaagaa agtttatgag
    35581 attgccatct cccaggcgtt gaaaaatatc gaccagattt tcagcgaatt accgacaaac
    35641 tcgcagcttt ttgccgcaga tagcggcgtg aagcttggca aacaggaagt gcaaagcggc
    35701 agcgtgatga gccacctgat ggcgggcgtc tctgcggcgc tgccgtttgt catcggcggt
    35761 ggtatcctgg tggcgcttgc caacatgctg gtgcagttcg gtttgccgta taccgatatg
    35821 tcgaaaggcg cgccgtcgtt tacctgggta gttgaatcca tcggttacct tggcttcacc
    35881 tttatgatcc ccatcatggg cgcttatatt gcctcgtcga ttgccgataa accggctttc
    35941 gctccggcgt ttctggtttg ctatctggcg aacgacaaag cgctgctcgg cacccagtcg
    36001 ggtgcgggat tcctcggcgc ggtggtgctg gggctggcga ttggctattt cgtcttctgg
    36061 ttccgcaaag tgcgccttgg caaagcatta caaccgctgc tcggttcaat gctgatcccg
    36121 ttcgttactc tgctggtttt cggcgtgctc acttactacg ttatcgggcc ggtgatgtcc
    36181 gacctcatgg gcgggctgct ccacttcctg aataccattc ctccatcaat gaagtttgcg
    36241 gcagcgtttc tggtcggtgc gatgctggcg ttcgatatgg gcggccccat caataaaacc
    36301 gcctggttct tctgcttctc actgctggaa aaacacattt atgactggta cgccatcgtc
    36361 ggcgttgtcg cgttgatgcc gcccgttgcg gcgggtcttg cgacgttcat tgcgcccaaa
    36421 ctgtttaccc gacaggaaaa agaggctgcc agtagtgcca ttgtggtcgg tgccacagtc
    36481 gcaaccgaac cggctatacc ttacgctctg gcagctccgt tgccgatgat taccgccaat
    36541 acgctggcgg gtggcattac cggcgtgctg gtgatcgctt tcggaatcaa acgtctggca
    36601 cctggtttgg gtatctttga cccgctgatc ggcctgatgt cgccggtggg ttcgttttat
    36661 ctggtgctgg cgattggcct cgcgctgaac atttcattca ttatcgtcct gaaaggattg
    36721 tggttacgcc gcaaagcgaa agccgcgcaa caggagcttg tccatgaaca ttgagttact
    36781 gcaacagttg tgcgaagcca gcgccgtcag cggcgatgaa caggaagttc gcgacattct
    36841 gataaacacg ctggaacctt gcgttaatga gatcaccttt gatggtctgg gcagctttgt
    36901 tgcccgtaag gggaataaag gtccaaaagt tgccgttgtc gggcatatgg atgaagtcgg
    36961 ctttatggtc acccacatcg acgagagcgg ttttctgcgc tttaccacca ttggcggctg
    37021 gtggaatcag tcaatgctca accaccgggt aaccatacgc acacacaagg gatttaaaat
    37081 ccctggtgtg attggttccg tcgcacctca tgcgttaacg gaaaagcaaa agcaacagcc
    37141 gctgtcattt gatgagatgt tcattgatat tggcgcgaac agtcgcgatg aggtggaaaa
    37201 acgcggtgtg gcgatgggcg attttattag cccggaagcc aattttgcct gctggggcga
    37261 agataaagtg gtcggcaagg cgctggataa ccgcatcggc tgcgcaatga tggctgaact
    37321 attgcagacg gtgaataatc ccgaaattac gctgtatggc gtcggcagcg tggaagaaga
    37381 agttgggcta cgcggggcac aaacctcggc ggaacacatt aaaccggatg tggtgattgt
    37441 gctggatacc gctgtcgcag gtgatgttcc gggcattgat aacattaaat acccgctgaa
    37501 actgggccag gggccggggc tgatgctgtt tgacaagcgc tacttcccca accagaaact
    37561 ggtggcggcg ttaaaaagct gtgccgcaca taacgattta ccgctgcaat tttccaccat
    37621 gaaaaccggt gcgacggatg gcgggcgcta caacgtgatg ggcggcgggc gtccggttgt
    37681 tgcgctgtgc ctgccaaccc gctatctgca cgctaacagc ggcatgattt caaaagccga
    37741 ttacgatgct ctgctcacgt tgatacggga ttttctgacc accttaaccg cggagaaagt
    37801 caacgcgttt agccagttcc gtcaggtgga ttaatgctaa acgaacgcca gttaaagatt
    37861 gtcgatctgc tggagcaaca gccgcgcacg cctggtgagc tggcgcaaca gactggcgtt
    37921 tcaggcagga ccatcctgcg tgatattgac tatctcaact ttacccttaa cggcaaagcc
    37981 cgcattttcg ccagtggcag tgcgggctat cagctggaaa tcttcgagcg ccgcagcttt
    38041 tttcagttgc tgcaaaagca cgataacgac gatcggctgc tggcgctgtt attactgaat
    38101 acctttactc cccgtgcgca actcgcctcg gcgcttaatt tgccagaaac ctgggtagca
    38161 gagcgtctgc cccggttaaa acagcgttat gaacgcactt gttgcctggc cagtcgcccc
    38221 ggtttgggcc atttcattga tgagacagaa gagaaacgcg ttatcttgct ggcgaacttg
    38281 ctgcgcaaag atccgttttt aattccgctg gcgggcgtaa cacgagacaa ttttcagcat
    38341 ttatccacgg cctgcgacaa ccaacaccgc tggccgctca tgcagggtga ttatctctcc
    38401 agcctgattc tggcgattta cgccctgcgt aatcaactga ccgatgagtg gccgcaatat
    38461 cccggtaacg agataaaaca aatcgttgaa cagagcggca tgtttcttgg tgataacgct
    38521 gtaagaaccc tgacaggttt gatagagaaa cagcatcagc aagcgcaggt aatttcagcc
    38581 gatcatgtgc tggggttgct gcaaagggtg ccaggcatcg cgtcattgaa tattattgat
    38641 acgcagctgg ttgagaatat taccgggcat ttattacgtt gccttgccgc accggtgtgg
    38701 attgccgagc accgccaaag cagcatgaat aacctgaaag ccgcctggcc tgcggccttt
    38761 gatatgagtc tgcactttat tacgctactg cgtgaacagc tcgatattcc ccttttcgat
    38821 agcgatctga tcggtttgta ttttgcctgt gcgctggaac ggcatcaaaa cgaacgccag
    38881 ccgatcattt tgctctcgga ccagaacgcg attgccacta ttaatcagct cgccattgag
    38941 cgcgatgttt tacattgtcg ggtgattatt gcccgtagct taagcgagct tattgccatt
    39001 cgcgaagaga ttgagccgtt attgatcatt aacaacagcc attatttact ggatgacgcg
    39061 gtaaataatt acatcaccgt aaaaaatatc attacggctg ccgatatcga acaaataaaa
    39121 cactttctgg cgacggcatt tattcgccaa cagccggaac gctttttctc tgcccccgga
    39181 agttttcatt attcgaatgt acgcggtgaa agctggcaac atattacccg gcaaatttgt
    39241 gcgcaattag tggcacaaca ccatattacc gccgatgaag cgcaacggat catcgcccgc
    39301 gaaggcgaag gtgaaaacct gattgttaat cgcctcgcca tcccacattg ctggagcgaa
    39361 caggagcgac gttttcgtgg attttttatt accctcgccc aaccagttga ggtgaataac
    39421 gaagtcatta accatgtctt gatcgcctgc gccgccgccg atgcgcgtca tgagctaaaa
    39481 atatttagct atctggcaag cgtattgtgt cagcatccgg cagaagttat tgccgggtta
    39541 acaggatatg aggcatttat ggagttactt cacaaggggt gaactgtgtc ggatgcgccg
    39601 catccgacac tgcatttatc atcaaaaacg tttaacgcag gctattatag tcatcaacca
    39661 tttcgttgta ctcgcgtaac gctcgtggaa atgagtcttc gaccatccag cttgtgtttg
    39721 catcctgaag atgctcttta tcccagtcag agtacgggac tttatctctg acgcggcgaa
    39781 catatttttt agtctcgagt tgatactggt ctgccgaatt aatgaacgag aaattcatac
    39841 cgcctttatc cgcttctttg gcctgcgcta ccagcgtttc cagttcagag actttcttca
    39901 ttgccgcatc aacatcaaac ttctcttgcg aaatcaggtt attaatttgt ttcgctgaaa
    39961 tcatgactgc cagagagtag tagtggaatg ttttcccttc tctttcttca atatttttca
    40021 gttccgcaag ctggcgctta tcattaattt cctgaatcgc cgcatgataa gattctgcta
    40081 caggttcaaa ggattccaga ttttttaaga atgtctggtg cagcgttttc cctttcgcaa
    40141 acgcatcgtc tttataattt tcctgggtgt aatatttatc catttcgtta atggtattac
    40201 ccaacgccac ggcagcatca atataactca ccgccacgcc atcaataggt tgcagcgcag
    40261 gcgttaacgc cacagcactt tttacgcctt tttcacactc agcgaggttg gattcactaa
    40321 tgccgtagat gccataaaca gtactctctt taccggtagg cccctgttta aaatctttca
    40381 gccagtcagc ataacgcgcc agactgttct gtacagggat ctgtaacttg ttgtagcact
    40441 tgatatagac atccattttt tcgtcgaaaa ccgcttgttc atcgaccacc ggttgcgcag
    40501 gtgtttcagg tttagcttct gcttttgcaa cgggagcttc aggagctggt gcggcaggtt
    40561 gactattggc aggcgggggc gtttctgttt cggctttttt atcatcacaa cccgtcagac
    40621 cgagggccat gatggcgact ataatagcgg aagataataa attccttttc atttaattgc
    40681 cttataaata tatagataag agagtgaaca taataaatga aaaaagaaaa cgccactaca
    40741 cgcgttttat ttataaataa aataataaaa ttcacgaaat gaataagtcc tgcaatttaa
    40801 tatatatcgc aggacttatt ttaattaatt gctcaaacgc tgcggatggg tataaaccgt
    40861 tgcccgaccc ggtttacaaa aacctaccag tgtcagatta cagcgctcag ccacttctac
    40921 agcaagcgtg gtcgcggcag acaccgcaaa caaaatctct acgccgcaca tcgccgactt
    40981 ttgcaccatt tcataactgg cacgactgga aaccagtacc gcaccttgct gccagctttc
    41041 cccttcttgt gaccggcggc ctaacagttt gtccagcgcc acatggcgac ccacgtcttc
    41101 atgcccgccg accagttcgc cagatggcaa catccaggca gcggcgtgag tacaaccagt
    41161 cagttgcccc actggctgaa aatcgttgag atgacgtaat gcatcatcca gtttgttgag
    41221 atcaaacgtc tgggtgaacg gtagcggctg caccggtttt ccgatgtcat taagttgctc
    41281 cacgccgcat acgccacagc ccgtacgtcc cgccagcgcc cggcggcgct ccttcaaccc
    41341 cataaagcgg cggctggaaa gctcaatttg tacttcaaga ccattacagg aaggaacgac
    41401 atccatgccg aagatatcgc gcggactttc tattatcccc tcggaaagcg aaaaaccgag
    41461 cgcaaagtac tcaaggtctt tgggcgacgc catcatcacc acatgcgaaa tgccgttgta
    41521 gaccagcgca acgggaactt cttccgcgac ctcatctaac cgtgggtgtt gtaaatcatc
    41581 acgccgccat aactcaattt gacgaacacc tgtgatattt gtcacatttt cgatttcttt
    41641 tcgctgtgtt tttttcactt tctcttaacc agataagaac acacacacca acattatggt
    41701 attctgttac aaacccttcc tggatggagg gaaattgagc caattctgga cctttgcggc
    41761 cccttccgca aagaaaaata actcccactc cctgcacacg cagcaagcga atgtaaatgg
    41821 gacgtgacaa tgtcgaaaca aggagcaatc catgcaggtc agcagaaggc agttctttaa
    41881 gatctgcgct ggcggtatgg caggcaccac ggcagcggca ctgggttttg cacccagcgt
    41941 agcactcgcg gaaacccggc agtataaact gctgcgcacc cgcgaaaccc gtaatacctg
    42001 cacctattgt tccgtaggct gtgggctgtt gatgtacagc ctcggtgacg gagcaaaaaa
    42061 cgccaaagca tctatcttcc atatcgaagg cgatccggat cacccggtca accgcggtgc
    42121 actttgtccg aaaggcgctg gcctggtgga tttcatccac tccgaaagcc gtctgaagtt
    42181 tccggaatac cgtgcgccag gttctgataa atggcagcaa atcagttggg aagaggcgtt
    42241 tgatcgcatc gccaaactga tgaaagaaga ccgcgatgct aactacattg cgcaaaacgc
    42301 cgaaggcgtg actgttaacc gctggctctc caccgggatg ctgtgtgctt ccgcgtcgag
    42361 taacgaaacc ggctatttaa cgcaaaaatt ctcccgcgcg ctgggtatgc tcgcggtcga
    42421 caaccaggcg cgtgtctgac acggaccaac ggtagcaagt cttgctccaa catttggtcg
    42481 cggtgcgatg accaaccact gggtcgacat caagaacgcc aacctcgtcg tggtgatggg
    42541 cggtaacgcc gctgaagctc acccggtcgg gttccgctgg gcgatggaag ccaaaattca
    42601 caatggcgcg aagctgattg tgatcgatcc tcgctttacg cgtacggcgg cggtggctga
    42661 ctactatgcc cctattcgtt ccggtactga cattgctttc ctgtcaggcg tattgctgta
    42721 cctgctgaac aatgaaaaat tcaaccgcga atacaccgaa gcctatacca acgccagcct
    42781 gatcgtgcgt gaggattacg gctttgaaga tggcctgttc accggctatg acgcggaaaa
    42841 acgcaagtac gataaatcct cctggactta cgaactggac gaaaacggct tcgccaaacg
    42901 cgataccacg ctgcaacatc cgcgctgcgt gtggaacttg ctgaaacagc acgtttcccg
    42961 ctacacgcca gatgtggttg aaaacatctg tggtacgcca aaagacgcgt tcctgaaagt
    43021 ctgcgaatac atcgcagaaa ccagtgctca cgataaaact gcctcgttcc tgtatgccct
    43081 cggctggacg caacactccg ttggtgcgca aaacattcgt acgatggcga tgatccagct
    43141 gctgctcggc aatatgggga tggcaggcgg cggcgttaac gccctgcgcg gtcactccaa
    43201 tattcagggg ctgacggacc tggggctgct gtcgcagagc ctgccaggtt acatgacgct
    43261 gccaagcgag aagcagaccg atctgcaaac ctaccttacc gccaacacgc caaaaccgct
    43321 gctggaaggc caggtaaact actggggcaa ctacccgaaa ttcttcgtct ctatgatgaa
    43381 ggccttcttt ggtgataaag cgacggcgga aaatagctgg ggctttgact ggttgccgaa
    43441 gtgggataaa ggctacgacg tcctgcaata cttcgagatg atgaaagagg gcaaggtcaa
    43501 tggctatatc tgccagggct ttaaccctgt tgcctcattc ccgaacaaaa acaaagtgat
    43561 cggttgtctg tcgaaactga agttcctcgt caccatcgac ccgctgaaca ctgaaacctc
    43621 taacttctgg cagaaccacg gtgagctgaa cgaagttgac tcgtcgaaga tccagaccga
    43681 agtgttccgt ctgccatcga cctgcttcgc agaagagaac ggttcaatcg ttaactcagg
    43741 tcgctggttg cagtggcact ggaaaggtgc ggacgcccca gggattgcgc tgactgatgg
    43801 cgagatcctc tccggtatct tcctgcgctt gcgcaagatg tatgccgaac agggtggcgc
    43861 aaacccggat caggtgctga acatgacctg gaactacgcc attccgcatg agccgaagtc
    43921 ggaagaagtg gcgatggaga gcaacggtaa ggcgctggcc gatattaccg atccggcaac
    43981 cggtgcggtt atcgtcaaga aaggccaaca acttagctcg ttcgcccaat tgcgcgatga
    44041 cggtacaacc tcctgtggct gctggatttt cgccggtagc tggacgccgg aaggcaacca
    44101 gatggcgcgt cgtgataacg ccgatccgtc tggcctcggt aacacgctgg gctgggcatg
    44161 ggcatggccg cttaaccgcc gcattctgta taaccgcgcc tccgcagatc cgcagggtaa
    44221 cccgtgggat ccgaagcgtc agttgctgaa atgggacggc actaagtgga ccggctggga
    44281 tattccggac tacagcgcag cgcctccggg cagcggcgtc gggccgttta tcatgcagca
    44341 ggaaggcatg gggcgtctgt ttgccctcga taagatggcg gaaggtccgt tcccggaaca
    44401 ctacgagccg tttgaaacgc cgctgggaac taacccgctg catccaaacg ttatctcgaa
    44461 cccggctgcg cgaatcttta aagacgacgc cgaagcattg ggtaaagccg ataagttccc
    44521 gtatgtcgga accacctatc gtctgaccga gcacttccac tactggacca aacacgcgct
    44581 gttgaacgcg attttgcaac cagagcagtt tgtggaaatc ggggagtcgc tggcgaataa
    44641 acttggcatt gcccagggcg ataccgtgaa agtctcctcc aaccgtggct atatcaaagc
    44701 caaagcggtg gtgaccaaac gtattcgcac gctgaaagcg aacggcaaag atatcgatac
    44761 catcggtatt ccgattcact ggggctatga aggcgtagct aaaaaaggct ttattgccaa
    44821 tacgttaacg ccattcgttg gtgatgcgaa cacgcagacg ccggagttta agtccttcct
    44881 tgtgaatgtg gaaaaggtgt aacggagacg acatatggct tatcaatcgc aagatatcat
    44941 tcgtcgttcc gcgactaacg gtctgacccc cgcgcctcag gcgcgggact tccaggaaga
    45001 agtggcgaaa ctcatcgacg tcaccacctg tatcggctgt aaagcctgtc aggtggcgtg
    45061 ttcagagtgg aacgacattc gcgataccgt cggcaataac attggggtgt acgacaaccc
    45121 caatgattta agcgccaaat cgtggacggt gatgcgcttc tcggaagtgg agcagaacga
    45181 caaactggaa tggctgatcc gcaaagacgg ctgtatgcac tgttccgatc caggctgcct
    45241 gaaagcgtgc ccggcggaag gtgcaattat tcagtatgcc aacggtatcg tcgacttcca
    45301 gtccgagcag tgcattggct gcggttattg cattgcgggc tgtccgttcg acattccgcg
    45361 cctcaacccg gaagacaacc gcgtctacaa atgtacgctg tgcgttgacc gcgtggtggt
    45421 tgggcaagaa ccagcctgcg tgaagacctg cccaacgggc gcgattcact tcggtacgaa
    45481 agagtcgatg aaaacgctgg cgagcgagcg cgttgctgag ctgaaaaccc gcggttacga
    45541 caatgcgggt ctgtacgatc cggcaggcgt cggtggtaca cacgtcatgt acgtgctgca
    45601 ccatgctgac aagccaaatc tgtatcatgg cttgccggag aacccggaaa tcagcgaaac
    45661 cgtgaaattc tggaaaggca tctggaaacc gctcgcggct gttggctttg cggctacctt
    45721 tgcggccagt atcttccact acgtgggtgt cggtccgaac cgtgcggatg aggaagagaa
    45781 taatctgcac gaagagaaag acgaggagcg caaatgaaac gacgtgacac catcgtgcgc
    45841 tacaccgcgc cggaacgtat caaccactgg atcaccgcct tctgcttcat cctggcggcg
    45901 gtgagcgggc tgggcttttt gttcccgtcc ttcaactggt tgatgcaaat catgggcaca
    45961 ccgcagctgg cgcgaattct gcacccgttt gtcggcgtgg ttatgtttgc ctcgttcatc
    46021 atcatgtttt tccgttactg gcatcacaac ctaatcaatc gggatgatat cttttgggcg
    46081 aagaatattc gtaagatcgt cgtcaacgag gaagtaggtg acaccgggcg ttataacttc
    46141 ggtcagaaat gcgttttctg ggcggcgatt attttcctgg ttctgctgct ggtgagcggc
    46201 gtgattatct ggcgtcctta ttttgcgcct gctttctcaa tcccggtgat ccgattcgcg
    46261 ttaatgctgc attcatttgc cgcagtggcg ttaattgtgg ttatcatggt gcatatctac
    46321 gccgcccttt gggtgaaagg cacgattacc gccatggtgg aaggatgggt taccagcgcc
    46381 tgggcgaaga aacatcaccc gcgctggtac cgtgaggtcc gcaagacaac ggaaaagaaa
    46441 gctgaatgag tattcgcata atcccgcaag atgagctggg ttcgagcgag aaacgtacgg
    46501 cggatatgat tccgccgtta ttgttccctc ggctcaagaa tttatacaac cgccgcgccg
    46561 agcgtctgcg cgagctggca gaaaataatc cgctgggtga ttacctgcgc tttgctgcgc
    46621 ttatcgccca cgcccaggaa gtggtgctgt acgaccatcc gctggagatg gatctgaccg
    46681 cgcgcattaa agaagccagc gcacagggca agcccccgct ggatattcac gttctgccgc
    46741 gtgataagca ctggcaaaag ctgctgatgg cgctaattgc tgagctgaaa cctgaaatga
    46801 gcggcccggc gctggcagtg attgagaatc tggagaaggc atcgactcag gagctggaag
    46861 atatggccag cgcgctgttt gcctctgatt tctcgtccgt cagcagcgat aaagcgccgt
    46921 ttatctgggc tgcgctgtcg ctctactggg cgcagatggc caatctgatc cccggcaaag
    46981 cccgcgctga atacggcgaa caacgtcaat attgcccggt atgtggctct atgccggtgt
    47041 ccagcatggt gcaaattggc accactcagg gtctgcgtta cctgcactgc aacctgtgtg
    47101 aaaccgaatg gcacgtagtg cgcgtaaaat gcagcaactg cgagcagagc ggcaaactgc
    47161 attactggtc gctggatgac gaacaggccg cgattaaagc cgaaagctgc gatgactgcg
    47221 gcacttacct gaagattctt tatcaggaaa aagaaccgaa agttgaagcc gtggcagatg
    47281 acctcgcctc tctggtactg gacgcgcgaa tggagcaaga aggctacgcc cgtagttcca
    47341 ttaacccgtt cctgtttccg ggtgaaggag agtaatctct gacgttttcc gggtggcgca
    47401 ggtcgcccgg attacagctc atagggtgtg acgacaccat ctctcgtatt ccgcgttacc
    47461 cattcaagat catgccgcaa ttgtctgttt gcctcgttct tccctccaac acatcgataa
    47521 ggttatgggt atgtcactga tgacaagcgt atgattgcct gatgcgctgc gcttatcagg
    47581 cctaccaata aacttcaata tattgaattt gcatgacttt gtaggccgga taaggcgttc
    47641 acgccgcatc cggcatgaac aacgcgcacg ttgtcagcaa tctgattgac atcatttttt
    47701 cccattacca ttcctcgcgc agcttacgct gatattcgac accatcttcc tcacaccctt
    47761 gccagatgcc aggaacactg gttcttgctg tttgcgattg atttatcagg tattgatcta
    47821 ccgcttccct taataggtcc gcacgaggaa gattacgctg cacctcgaga tcatcaagtt
    47881 gcttaatcac ctcattcgat aaatcgagta aaattctgcc catatctata cctgccagag
    47941 agttcataaa tatctcgcca atatcatttt tgaatctatg gagagaaaag tacccttgcc
    48001 gaatctttaa agaaagctca tttacgcatc attttttatt tttgcgaggc gttttccaga
    48061 aataataaga gcgttgcagt tgtttcattt attactcaaa aatggtttag ttatcgccca
    48121 ggatgagtta tgggtgctat gaatatatta tattcaggga tatttatttg cataatctga
    48181 atggtgtagc cgaaaaacgt cagcatccta acagcaccag gatttaaggt gaaattaatc
    48241 tttcatcaca gaccggtcag tactgccacc aaccggtctt tacttgctca tcgttataat
    48301 tttgttggta acagttcggg aaagttgttt ttctaaatta acaacagaat aactttcttg
    48361 tttaatgaga ttaatattgg caaccattat agcctccttc ccacggttta agagcaaaca
    48421 tacaacataa tgattcataa catacagagc agtaaatgaa tgatactgac aatgatgcca
    48481 gcccattatc tttgcataat gtttgtgagc tatagatttt aaaaatattt atgagaaaag
    48541 tatggcacca gaggaggttt tgactatcaa aaatcgctca aacctgaact aagatgatga
    48601 gttaagcatt atctgctata attgccactc agctctacaa ataatattta aatgctcctg
    48661 aacagtgatg tagcaagaca cgtatggtgc caacgaagaa taaaaattta tagagttctt
    48721 attcaatatt accggcatag acagggaatg atgtaaatat ggccatgaat ggcgttggat
    48781 g
//
LOCUS       DAKISA010000020        48065 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.020, whole genome shotgun sequence.
ACCESSION   DAKISA010000020 DAKISA010000000
VERSION     DAKISA010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48065
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.020"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(49..273)
                     /gene="cspD"
                     /locus_tag="OVW45_001182"
     CDS             complement(49..273)
                     /gene="cspD"
                     /locus_tag="OVW45_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCV5525687.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            678..1916
                     /locus_tag="OVW45_001183"
     CDS             678..1916
                     /locus_tag="OVW45_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCV5525688.1"
                     /translation="MSSAIHRLSDTLLRKLSGSPTTKNAFFNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRQVSPERLRLGNYPDLSLKAAREKAAQCRAWLAEGKNPRYELNRAVQD
                     ALAPVTVKEALTYWLESYAKEKRTDYESLKSRINKHIISQIGALPLEKCELRHWLACF
                     DQMAKRSPVSAGFLLQVCKQALKYCRKRRYAISNVLDDMVVGDVGKKAEISERVLTNK
                     ELGELLRALDEKIYPPYYSALIRLLIVFGCRATELRRSEVQEWDFKEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTKLVEQNRHTGLLLGELKGQSSVSEYGRTAHRRINQAPWTL
                     HDIRHTFTTMLNDLGVDPHVVEQLTAHQLPGVQRVYNHSRYLDAKRDALNLWVERLEL
                     LQNNDEKIVVMTPRIYSQNS"
     gene            2326..2535
                     /locus_tag="OVW45_001184"
     CDS             2326..2535
                     /locus_tag="OVW45_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HCV5525689.1"
                     /translation="MREINEDRVIREDECRKLTGVCRTTRYELEKKGRFPSRLNLGGR
                     SVGWLLSEVMEWVKSRDRINSGKAV"
     gene            complement(2647..2823)
                     /locus_tag="OVW45_001185"
     CDS             complement(2647..2823)
                     /locus_tag="OVW45_001185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525690.1"
                     /translation="MTNEESTKKTSPARKRRRRKKEHESERFAPCSFALEKYLKEYSS
                     KERASQLWQRTETD"
     gene            complement(2881..3333)
                     /locus_tag="OVW45_001186"
     CDS             complement(2881..3333)
                     /locus_tag="OVW45_001186"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525691.1"
                     /translation="MRGFVVSIAELHVSALVVFTLHLGEYHFKSFLVFTFVLHCIPLL
                     MGGANMSRSSVNNKSQQLNARFPHEVVSGIEASLQPGETKANFIVTAVRGEIARRQAE
                     GSGENPLVSSLDALANVEQIGIKAAEEIGQLITVAREELQRRKAKESE"
     gene            3387..3584
                     /locus_tag="OVW45_001187"
     CDS             3387..3584
                     /locus_tag="OVW45_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCV5525692.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARN
                     RCPSGCVAVFAARIRQEVCHA"
     gene            3577..3897
                     /locus_tag="OVW45_001188"
     CDS             3577..3897
                     /locus_tag="OVW45_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525693.1"
                     /translation="MPDMTNYQYLINPHFNCEHDIAKKVYSAADGATDNISMAVASIG
                     SLMWHASENEEYDEKAMRIDMGNIGLLLAMLGQFDISLRCTIENATDALNAIEKANTD
                     SNLR"
     gene            3904..4203
                     /locus_tag="OVW45_001189"
     CDS             3904..4203
                     /locus_tag="OVW45_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCV5525694.1"
                     /translation="MRTYLSGLTASGYAHPKIIPGAIYLDKNGNRVTVKELMFDRVYF
                     IRDGYSFHSSLNVEIFISRFRREIPLSRNNHVSCMDVDKKLQELKNMIAAWREQK"
     gene            4200..5990
                     /locus_tag="OVW45_001190"
     CDS             4200..5990
                     /locus_tag="OVW45_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCV5525695.1"
                     /translation="MKKAPNLKHQPRDKMTEVIIFAGNDAWAHAKQWQEQDGRLAGDS
                     VPPVWLGEQQLAELDKLQIVPDGRYRVRLYQAGLLRPGLVNTIGQKLAAAGVRDADYY
                     PEGMHSQKRENWREYLERERAEQAEKKKVVELPVKKKERVKDDNASSLALNQMGASQR
                     GEVLLAHYGGELAIHADSDTVHHYNGVVWEPVQDKELQRAMAQIFIDAEISYSQNAIK
                     SAVDTMKLSLPVMGNTARNLIGFSNGVFDTRTGNFREHNKNDWLLIASELPFSPPAEG
                     ETLATHAPNFWKWLRRSVAENDRKADRVLAVLFMVLANRYDWQLFIEVTGPGGSGKSV
                     MAEICTMLAGKANTVSASMKALEDARERALVVGFSLIIMPDMTRYAGDGAGIKAITGG
                     DKVAIDPKHKAPYSTRIPAVVLAVNNNAMSFSDRSGGISRRRVIFNFSEVVPENERDP
                     MLAEKIEGELAVVIRHLLTRFSDQDEAKRLLYEQQKSEEALVIKREGDSLVDFCGYLM
                     SSVMCDGLLVGNAEIIPFSPRRYLYHAYLAYMRAHGFGKPVTLTRFGKDMPGAMAEYG
                     REYMKRKTKHGLRSNVTLTEDSEDWMPSCA"
     gene            6305..6550
                     /locus_tag="OVW45_001191"
     CDS             6305..6550
                     /locus_tag="OVW45_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525696.1"
                     /translation="MTTKHAKKPQSHALDLTEHWLRVAIKIIDRNAGEGYAKAHPELI
                     SAFMTTTAANFATLTEREIAEAEQVTTINVKTGEQTA"
     gene            6547..6969
                     /locus_tag="OVW45_001192"
     CDS             6547..6969
                     /locus_tag="OVW45_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCV5525697.1"
                     /translation="MTAQIAAYGRLVADPQLKTTSKGTQMTMASMAVPLPCSQADDGT
                     ATIWLSVLAFGRQADALAKHQKGELVSVAGNMQVSQWTGQNGETRQGWQVIADSVISA
                     RTARPGGKKGQQGQATDALNRAKQQAGNDDPYGDNIPF"
     gene            7629..9509
                     /locus_tag="OVW45_001193"
     CDS             7629..9509
                     /locus_tag="OVW45_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCV5525698.1"
                     /translation="MKTWFSIKAMADVVHVRIYDEIGGYGVKASALTDEINACGNVPE
                     IHLRIHSPGGDIFEGLAIYNALKNHPAKKIVHIEGMAASMASFIAMCGDHIVMPENAM
                     MMIHAPRGVTAGVSGDVRRFADLMDKLGDTMAETYAGRTGRSKQEITAMMEAETWMDG
                     NECKANGFADEVIPAITAMARIESKRIGDFSNMPENIKSMISQKTGSGEQERLNGIRE
                     LFGTFNGRYNDLAISCLADSGCSVEDARERLLLTMGKESTPTNKTTPANLYYAYTDNG
                     NITGDAMRQGLNARLGHEQAERGNPYAMMSLFDMAQASLTHRGISTGSYSTRSQIVNA
                     AFTHSSSDFTYILAGGAEKSVLAGWEHSGETFRQWTKKGSLSNFHEARRVGLNGFSTL
                     NKVPEGAEYKYITTSDRGEPIALATYGNIFSITRQAIINDDLNQLTVIPQAMGRAAAR
                     TVGNLVHLVLTGNTKLSDGIPLFSKKHGNDIDNGVSTTGLGIARRAMRTQRDENGEVL
                     NIVPKFLLVPAALEDRALQMVNSTTPFGADTNSGSYNPYHKQLDIIVDPRLDEVSDKQ
                     WYMLSAQGTDTIEVAYLDGNDEPYLEQQEGFIVDGVAWKVRIDAGVAALDYRGMVRAI
                     SS"
     gene            9768..10049
                     /locus_tag="OVW45_001194"
     CDS             9768..10049
                     /locus_tag="OVW45_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525699.1"
                     /translation="MTEAEILGLIRRVAGISQQVDEQAMQPDSVTAENYARVVDEVMR
                     RDGIELNGVDMRNIRTRVLELLAYRRRSQQRRESAKNTYQWRKPEHLRR"
     gene            10689..11009
                     /gene="clpS"
                     /locus_tag="OVW45_001195"
     CDS             10689..11009
                     /gene="clpS"
                     /locus_tag="OVW45_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCV5525700.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            11040..13316
                     /gene="clpA"
                     /locus_tag="OVW45_001196"
     CDS             11040..13316
                     /gene="clpA"
                     /locus_tag="OVW45_001196"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCV5525701.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(13722..13809)
                     /locus_tag="OVW45_001197"
     tRNA            complement(13722..13809)
                     /locus_tag="OVW45_001197"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13773..13775),aa:Ser,seq:gga)
     gene            complement(14063..14281)
                     /gene="infA"
                     /locus_tag="OVW45_001198"
     CDS             complement(14063..14281)
                     /gene="infA"
                     /locus_tag="OVW45_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCV5525702.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(14566..15270)
                     /gene="aat"
                     /locus_tag="OVW45_001199"
     CDS             complement(14566..15270)
                     /gene="aat"
                     /locus_tag="OVW45_001199"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCV5525703.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGAPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWHEDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFISHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQK"
     gene            complement(15312..17033)
                     /gene="cydC"
                     /locus_tag="OVW45_001200"
     CDS             complement(15312..17033)
                     /gene="cydC"
                     /locus_tag="OVW45_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCV5525704.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTVGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(17034..18800)
                     /gene="cydD"
                     /locus_tag="OVW45_001201"
     CDS             complement(17034..18800)
                     /gene="cydD"
                     /locus_tag="OVW45_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCV5525705.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWAVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(18923..19888)
                     /gene="trxB"
                     /locus_tag="OVW45_001202"
     CDS             complement(18923..19888)
                     /gene="trxB"
                     /locus_tag="OVW45_001202"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCV5525706.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            20433..20927
                     /gene="lrp"
                     /locus_tag="OVW45_001203"
     CDS             20433..20927
                     /gene="lrp"
                     /locus_tag="OVW45_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCV5525707.1"
                     /translation="MVDSKKRLGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            21062..25129
                     /gene="ftsK"
                     /locus_tag="OVW45_001204"
     CDS             21062..25129
                     /gene="ftsK"
                     /locus_tag="OVW45_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCV5525708.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTAAQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVETAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPVAPQPQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            25284..25895
                     /gene="lolA"
                     /locus_tag="OVW45_001205"
     CDS             25284..25895
                     /gene="lolA"
                     /locus_tag="OVW45_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCV5525709.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            25906..27249
                     /gene="rarA"
                     /locus_tag="OVW45_001206"
                     /gene_synonym="mgsA"
     CDS             25906..27249
                     /gene="rarA"
                     /locus_tag="OVW45_001206"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCV5525710.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            27340..28632
                     /gene="serS"
                     /locus_tag="OVW45_001207"
     CDS             27340..28632
                     /gene="serS"
                     /locus_tag="OVW45_001207"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCV5525711.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            28871..31315
                     /gene="dmsA"
                     /locus_tag="OVW45_001208"
     CDS             28871..31315
                     /gene="dmsA"
                     /locus_tag="OVW45_001208"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HCV5525712.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLVNQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            31326..31943
                     /gene="dmsB"
                     /locus_tag="OVW45_001209"
     CDS             31326..31943
                     /gene="dmsB"
                     /locus_tag="OVW45_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCV5525713.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            31945..32808
                     /gene="dmsC"
                     /locus_tag="OVW45_001210"
     CDS             31945..32808
                     /gene="dmsC"
                     /locus_tag="OVW45_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HCV5525714.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDSWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(32844..33470)
                     /locus_tag="OVW45_001211"
     CDS             complement(32844..33470)
                     /locus_tag="OVW45_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCV5525715.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            33785..34933
                     /locus_tag="OVW45_001212"
     CDS             33785..34933
                     /locus_tag="OVW45_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCV5525716.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            35143..36573
                     /locus_tag="OVW45_001213"
     CDS             35143..36573
                     /locus_tag="OVW45_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCV5525717.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITAWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(36574..37482)
                     /locus_tag="OVW45_001214"
     CDS             complement(36574..37482)
                     /locus_tag="OVW45_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCV5525718.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKEQEIAVAGNI
                     ILDDVDAELDAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWITERPGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            37582..38172
                     /locus_tag="OVW45_001215"
     CDS             37582..38172
                     /locus_tag="OVW45_001215"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HCV5525719.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPVWWYSFPAMLKGYI
                     DRVWNNGLAYGDGQKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL"
     gene            complement(38254..38994)
                     /gene="pflA"
                     /locus_tag="OVW45_001216"
     CDS             complement(38254..38994)
                     /gene="pflA"
                     /locus_tag="OVW45_001216"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCV5525720.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(39186..41468)
                     /gene="pflB"
                     /locus_tag="OVW45_001217"
     CDS             complement(39186..41468)
                     /gene="pflB"
                     /locus_tag="OVW45_001217"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCV5525721.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(41523..42380)
                     /gene="focA"
                     /locus_tag="OVW45_001218"
     CDS             complement(41523..42380)
                     /gene="focA"
                     /locus_tag="OVW45_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCV5525722.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(42786..44546)
                     /locus_tag="OVW45_001219"
     CDS             complement(42786..44546)
                     /locus_tag="OVW45_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCV5525723.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            44676..45368
                     /locus_tag="OVW45_001220"
     CDS             44676..45368
                     /locus_tag="OVW45_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HCV5525724.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            45567..46655
                     /gene="serC"
                     /locus_tag="OVW45_001221"
     CDS             45567..46655
                     /gene="serC"
                     /locus_tag="OVW45_001221"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCV5525725.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            46726..48009
                     /gene="aroA"
                     /locus_tag="OVW45_001222"
     CDS             46726..48009
                     /gene="aroA"
                     /locus_tag="OVW45_001222"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCV5525726.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT        12329 a        11377 c        12588 g        11771 t
ORIGIN      
        1 atcgggctgg cattttgcct ttaggatgta cacaatgaga cagaagagct atgcgactgc
       61 cgcttctact tcgacgggca caataacact ggcgtgattg ccttttggcc cctggtggac
      121 atcaaactga acggattgtc cagcttttag cgttctgtaa ccatccatct gaatggtgga
      181 ataatgagcg aaaatatctt cgccgccgcc ttcagggcag atgaaaccaa accctttggc
      241 attgttgaac cacttaacag tacccttttc catgcttcga catccttcgc aaatcttata
      301 caagtaagat ggaataaacc ggggtcagag agggggctgt tcaaaacctc gccaactcta
      361 gaaatacaat ttagagaatt agggcgagcc gtcaagcatt tgacaggggc cagggggcag
      421 gtatgaatca aaaatttgaa gcagttaacg ctattgacag gaatgtgaca gatgtcgctg
      481 atgccaacga tagatgatag ttatttatca tctagtgttc gcgggtgttc gtggtagtgt
      541 tttgtgtgca ttcatcgcta tgattataaa ggaaaatttt gttttccttg ttcgctcttg
      601 ttcgtcacgg tttgcccttg tacgatgcac ggtgtagtca atggtgtagt taattttgat
      661 gaccggaaac gcgaaacatg agcagcgcaa ttcatagact tagcgacact cttttacgaa
      721 aattaagcgg atcaccaacc acaaaaaacg ccttttttaa tgacggtggc aatcttagcg
      781 taagacattc caccagcggc ctgttaacct ggtatttcac ttacagggcc ggaactggca
      841 ggcaggtatc accggaacgc ctgagactgg gcaactatcc cgatctgagc ctgaaagcag
      901 ccagggaaaa agcggcacag tgtcgcgcct ggctggctga ggggaaaaat ccgcgctatg
      961 agcttaatcg tgctgtacag gatgcgttag cccccgtgac agttaaggaa gcgctcactt
     1021 actggcttga atcgtatgca aaggaaaagc gcactgacta cgaatcactg aaaagccgga
     1081 tcaataaaca cataatcagc cagattggcg ctttgccgtt ggaaaaatgc gagctacggc
     1141 actggttggc atgttttgac cagatggcaa agcgaagccc agtatctgcc ggatttttgc
     1201 tacaggtatg caaacaggcg cttaagtact gccgaaaacg acgatacgca atcagcaacg
     1261 ttcttgatga tatggttgtc ggtgatgtgg gaaaaaaagc agaaataagc gagcgcgtac
     1321 taacaaacaa ggagcttggg gaattactcc gcgccctgga tgaaaaaatt tacccaccgt
     1381 actacagcgc cctgattcgc ctcctgattg tcttcggatg ccgtgccact gagctaagac
     1441 gctctgaggt ccaggaatgg gattttaaag aaatgctctg gaccgtgccg aaagaacaca
     1501 gcaaaacgaa ggtagcaata ttcaggccaa taccggaagc gatcttgccg ttcgtaacga
     1561 agctggtgga gcagaacagg cacacgggat tattgctcgg agaactgaaa gggcaatcat
     1621 ccgtatcaga gtacggaaga acggcacaca gacgcattaa tcaagccccc tggacgttgc
     1681 acgacatccg gcacacgttt acaacgatgc tgaacgattt aggcgtggat cctcatgtag
     1741 tggagcagct aaccgcgcac cagttgccag gagtgcaacg agtctataat cactcccgtt
     1801 atctcgatgc aaaacgtgac gctcttaatc tatgggttga gcgtctcgag cttctccaga
     1861 acaatgatga aaaaatcgtt gttatgaccc cgcgaattta ctcccaaaat tcttgacaaa
     1921 ttacggctgt ttttcttgcg aaaaatggcc ctctaaaata aatcgtcgaa tatcggcaaa
     1981 tatcgacgat ttatatgtgt caactatcga aaaaaattga aatgtcttct tctgagttct
     2041 tattctctgt ttcttgatta ttccaatacc tgatggttta tctatttgtt tttaatgagg
     2101 taattcaggt gtcatgccaa atggcgacat tttttgttgc gtcgcttaaa aactcgtgac
     2161 acgattttaa acactaacga gcttctacga accttagcga acaaagcctt tagcatggtt
     2221 gtgtttttaa tttaagaggt gaaaaagaat ctaagtaata tccgatctca tatatctaca
     2281 gctaagagaa aagtttatat acgtaatttt tatgtaggtt tttgtatgcg tgaaataaat
     2341 gaagatcgcg taatccgtga ggatgaatgt cgtaagttaa ctggagtgtg ccgcactaca
     2401 cgttacgaac tggagaaaaa agggcgcttt ccgtcacgtc ttaatctggg cggtcgttct
     2461 gtagggtggc tgctgtccga ggttatggaa tgggttaaaa gccgggatcg cattaattca
     2521 ggtaaggcag tgtaaagggg agcatatgac acataaaaca aaggcgacct ggggcggtcg
     2581 ccagtttgga aacactaaac ataagcatgt caatgatagc cgctatcagg ctggtgggca
     2641 atgctatcag tctgtttcag tgcgttgcca taactgcgaa gctctctcct ttgatgagta
     2701 ttctttaagg tacttctcaa gggcaaaaga acacggcgcg aatcgttctg attcatgctc
     2761 tttctttctg cgtcgtcttt ttcgtgcagg tgatgttttt ttggttgatt cttcgttggt
     2821 cattgtgtgc gcctgtaaag caatgcgccg gagttcctca caccacggcg ctgatagtga
     2881 ttattctgat tctttggcct tgcggcgctg gagttcttcg cgtgcaacgg tgataagctg
     2941 cccgatttcc tccgctgcct tgatgccgat ttgttcgaca ttagctaggg catccagtga
     3001 agacacaagg ggattttctc cacttccttc tgcctggcgc ctggcgatct caccgcgtac
     3061 agccgtaact ataaaattcg ctttagtttc ccctggctgt agggatgcct caatgccact
     3121 cactacttca tgtggaaatc tggcattcag ttgctgtgac ttattgttaa cagaactcct
     3181 ggacatgttt gcacctccca ttaataacgg gatgcagtgt aatacaaaag tgaataccaa
     3241 aaaagacttg aaatggtatt cacctaaatg taatgtgaat accacaagtg ctgatacatg
     3301 caattcagca attgaaacga cgaagccccg caagtgtcgt taccactcgc agggcttcta
     3361 accaacaacg taaacgagga gccgttatgg ttgccgtaaa tcatatacca caccttgtac
     3421 acacacaaac ggcctttgtg tggcgttttc tggccctgag tgccggagaa tctcaaatca
     3481 tccacgtaac cgcctggacg gaacgcgaag cgcgtaaccg ttgcccgtca ggttgtgttg
     3541 ctgtattcgc cgcccgtatt cgtcaggagg tgtgccatgc ctgacatgac caattaccag
     3601 tacctgatta atccgcattt taactgtgag catgatattg ctaaaaaggt ttattccgct
     3661 gcggatgggg ctactgacaa tatatcaatg gctgttgcgt caattggtag cctgatgtgg
     3721 catgcgtcag aaaatgagga atatgacgaa aaggctatgc gcattgacat gggtaatatt
     3781 ggcttgttac tggcaatgct tgggcagttt gatatttcgt tacggtgcac cattgaaaat
     3841 gccacagatg cattaaatgc catagagaaa gcgaatactg attcaaatct gagataaata
     3901 atcatgagaa cgtatttatc tggcttgact gccagcggtt atgcacaccc caaaattatc
     3961 cccggcgcta tttatctgga taagaacggt aacagagtaa cggtaaaaga actgatgttt
     4021 gaccgtgtgt attttattcg tgatggctat tcatttcata gttcgctgaa cgtggagatc
     4081 tttattagca gattcaggcg ggaaatcccg ctttccagaa ataaccatgt gtcatgtatg
     4141 gatgttgata aaaaactaca ggaactgaaa aacatgattg ccgcgtggag agagcagaaa
     4201 tgaaaaaagc gccaaattta aaacaccagc cgcgtgacaa aatgacggaa gtcatcattt
     4261 ttgcgggtaa tgatgcgtgg gcacatgcga agcagtggca ggaacaggac gggcgactgg
     4321 ctggcgacag cgtgcctcct gtctggcttg gagaacaaca acttgccgaa ctggataagc
     4381 tgcaaatcgt accggacgga cgctatcggg tgcgtctcta ccaggcgggg ttattgcgtc
     4441 cggggcttgt taataccatc gggcagaaac tggcagcggc aggtgtcagg gatgctgatt
     4501 attaccctga aggaatgcac agccagaaac gggagaactg gcgcgaatat ctggaacgtg
     4561 aacgggcaga gcaggcggaa aagaaaaagg tagttgaact gcctgtaaag aaaaaagagc
     4621 gggtaaaaga cgataacgct tcatcactgg cgcttaacca gatgggagca agtcaacgcg
     4681 gcgaagttct cctggcacat tatggcggtg aactggcgat tcatgctgac tctgacactg
     4741 ttcaccatta caacggcgtt gtatgggagc cagtacagga taaagaatta cagcgagcta
     4801 tggcacagat tttcattgat gcggagatca gctattcgca gaacgccatt aaatcggcgg
     4861 tcgataccat gaagttaagt ttgcctgtaa tggggaatac agcccgtaac ctgattggat
     4921 tcagtaacgg ggtatttgat accagaacag gtaattttcg ggagcataac aaaaacgact
     4981 ggttgttaat tgccagtgaa ttacctttca gcccaccagc agagggggaa acgctggcaa
     5041 cacatgcgcc gaatttctgg aagtggttac gccgttcggt ggctgagaat gaccgcaagg
     5101 cggatcgcgt actggctgta ttattcatgg tgctggcgaa ccggtacgac tggcagttat
     5161 tcattgaggt aacaggtcca gggggaagtg gtaaaagcgt gatggcggag atttgcacca
     5221 tgctggcggg taaggccaac acagtatcgg caagcatgaa ggcgctggaa gatgcaaggg
     5281 aacgcgcgtt agtggttggc ttttcgctga ttatcatgcc ggatatgacc cgctacgctg
     5341 gtgatggggc agggattaag gccattacag gcggtgacaa ggtggcaatt gacccgaaac
     5401 acaaagcccc ctactcaacg cgtattccgg cagtagtgct ggcggttaac aataacgcca
     5461 tgtcattcag tgaccgcagc ggggggatct cacgtcgtcg ggtgatattc aatttttcgg
     5521 aagttgtacc ggagaacgaa cgcgacccca tgctggcaga aaaaatagaa ggagagctgg
     5581 cggttgtgat tcgccacctg cttactcgtt tttctgacca ggacgaagct aaacgcctgc
     5641 tgtatgagca gcagaaatca gaagaagctc tggtgataaa acgcgagggc gattcgctgg
     5701 tggacttctg cggctatctc atgtcgtcgg taatgtgtga tggtctgtta gtgggtaatg
     5761 ccgaaattat tccgttcagt ccgcgcaggt atctttatca cgcctatctg gcatatatga
     5821 gggcacacgg attcggtaaa cctgtaacac tgacgcgctt cggtaaagat atgccggggg
     5881 caatggcgga atatggcagg gagtatatga aacggaaaac gaagcacggt ttgcgttcaa
     5941 atgtgaccct gacagaggat tcagaagact ggatgccatc atgtgcatag gtcacaaatg
     6001 acgatggaaa aaattaaact tatggaataa ctgttcacca ctgttcaccc tgcaataaat
     6061 gtcttttata tcagtatatt atagggtgaa cagttattta tgaactgttc accaaactat
     6121 tcactgttca cctttttgat tctttattga gctttaaggg tgaacagtgg tgaacagttg
     6181 gtgaatagtt tttgtgaaac tgttaaccca ttaacatcat gaataaaaag ggaaaattgc
     6241 aaaaggtgaa caggtgaagg gttaaaatgc aaaaatttta ttttattgct gtgaggtaaa
     6301 gcctgtgaca acgaagcacg ccaaaaaacc acaatctcac gcccttgatt tgacagaaca
     6361 ctggctgagg gtggcgataa aaatcatcga ccgcaacgcc ggggaaggat acgcgaaagc
     6421 acatcccgaa ctgataagcg cattcatgac cacgacggcg gcaaactttg ccacgctgac
     6481 agaacgggag attgccgaag cggaacaggt gacaaccatc aacgttaaaa ccggagagca
     6541 gacagcatga cagcacagat agcggcttac gggcggctgg tggctgaccc gcagttaaag
     6601 accaccagca agggtacgca aatgacgatg gcgagtatgg cggttcccct gccgtgcagc
     6661 caggcagatg acggaacggc gacgatatgg ttatccgtcc tggcgtttgg cagacaggct
     6721 gacgcactgg caaagcacca gaaaggcgag ctggtgagcg tggcgggtaa catgcaggta
     6781 agccagtgga caggccagaa cggagaaacg cggcagggct ggcaggttat cgcagacagt
     6841 gtaatcagtg ccagaacggc gcgaccgggc ggtaaaaaag gccaacaggg gcaggccact
     6901 gatgcactga acagggccaa acaacaggcg gggaatgatg atccgtacgg ggataacata
     6961 ccgttttaag caacgagtga cagaagccgg agcaatccgg cttttttgta ggtactcctg
     7021 gtgggggtgg cctgtccacg gggcgggcgg ttcgcggaaa aaggcgtatt ttttgatttt
     7081 tatgtcacca tcaccaccag tttaagttat tgatatatgg caaaataaaa atttttagtg
     7141 tcgaattagg ttgttttttg ttcgaagccg gaacgttccc gaaagcattt acaaaaaaca
     7201 ggcgcaaaaa aaagcgcccc gatggtgcca ccggaggcgc tttgtcagcg acaaaggata
     7261 ctgccactac cgaaatgatg agtcttaatt gtgctggcgt ggcaataatt agtcaattct
     7321 tttgctggta tgacaaaaat cagaaaacac ataatctgat ttttagatag aaaatgattt
     7381 atttaccagt tttatcgatc aataatgatg cccgttaatc ataacggagg cagacttatg
     7441 ccagagaaca acagcagaaa accggataaa agtgcaacag tgcacataga cgccggaact
     7501 atggagaagc tcgaacgcta tcagcagttc attaaaagtc atcaccctgg aatgccagta
     7561 cccacgaaag ggcagattgc gagaagcgcg gttgaatact ggtacagggc aacgttagga
     7621 gcctggctat gaaaacatgg ttttccatta aggctatggc tgatgttgta catgtacgca
     7681 tttatgacga gatcggcggg tacggtgtaa aagcatcggc acttactgac gagatcaacg
     7741 cgtgcggtaa tgtgcctgaa atccatcttc gcatccattc acctggtggc gacatctttg
     7801 aagggctggc tatctataac gccctgaaaa atcatccggc aaagaaaatt gtacacattg
     7861 aaggcatggc ggcttctatg gcctcgttta ttgccatgtg tggcgatcac atcgttatgc
     7921 ctgaaaacgc gatgatgatg atacatgccc cccgtggtgt tactgccgga gtgtcgggcg
     7981 acgttcgccg ctttgctgac ctgatggaca agctgggcga cacgatggcg gaaacctacg
     8041 ccggaagaac gggcaggagc aaacaggaaa tcaccgccat gatggaggcg gaaacctgga
     8101 tggatggcaa tgagtgtaag gctaatggct tcgcagatga ggttataccc gcgattacag
     8161 caatggccag aattgaatca aaacgaatcg gagatttttc gaatatgccg gaaaatatta
     8221 aaagcatgat cagccagaaa actggcagcg gagaacagga acgactcaac gggatacgtg
     8281 aattgtttgg cacgttcaat ggaagatata acgacctggc tataagttgt cttgctgatt
     8341 caggctgtag cgttgaggat gcacgcgaac gccttttact cactatgggt aaagaatcga
     8401 caccgacaaa caaaaccacc cccgcaaatc tttactacgc gtacacggat aacggcaaca
     8461 taaccggcga tgcaatgcgt caggggctta atgcgcgtct gggacatgaa caggcagaac
     8521 gcggcaaccc ttacgccatg atgagccttt tcgatatggc acaggcatca ttaacccatc
     8581 gcggcataag cacaggcagc tacagcacac gctcgcagat agtaaacgcg gcattcaccc
     8641 acagcagcag cgattttact tatatccttg ctggtggtgc tgaaaaatcc gtgcttgccg
     8701 gatgggaaca cagcggcgaa acgttccgcc agtggacgaa aaaaggttca ttgtcaaact
     8761 tccatgaggc acgtcgtgtt ggcctgaatg gcttctctac attaaacaaa gtgccggaag
     8821 gcgcggaata taaatacatc accaccagtg accgtggcga accaatcgcg ctggcaactt
     8881 acggaaatat tttcagcatt acccgacagg cgattattaa cgatgacctg aatcagttaa
     8941 cagttatccc acaggcaatg gggcgagcag ccgccagaac ggtgggtaat ctggttcatc
     9001 tggtgctgac aggaaacaca aagctttctg acggtatacc gctgttcagc aaaaaacacg
     9061 gaaatgatat tgataacgga gtcagcacta cagggcttgg cattgcacgc cgtgcaatgc
     9121 gcacacagag ggatgaaaac ggtgaggtgc tgaatattgt gccaaaattc cttcttgttc
     9181 ctgcggctct ggaagacaga gcgttgcaaa tggttaactc aaccacgcct ttcggtgccg
     9241 atacaaacag tggttcatat aacccgtacc acaaacaact ggatattatt gtcgatcccc
     9301 gccttgatga agtcagtgat aagcaatggt acatgctttc agcacaggga acggacacaa
     9361 ttgaggtggc ttatcttgac ggtaatgacg agccttacct tgagcagcag gagggcttta
     9421 ttgttgacgg tgtggcctgg aaggtccgca ttgatgctgg cgttgctgct ctggattatc
     9481 gcggaatggt cagagcgatt tcttcatgag aacaaggcgg cagcagccgc cttttttgcg
     9541 ggtcctcctg gtgggggggg cctgtccacg gggcgggtcg gcgcggaaaa aagcgcattt
     9601 ttgtggtttt atcgtcatca tcatcatgtg tgtaacctgt tgtttttaat gtggttgatg
     9661 caaaaaagat gatgattgtg gttaattttt gttcgacatc ttttagcgtg acagattctt
     9721 tacaaaaaat ctgagcttgt tttcttaacc agcgcgatgg gggcacaatg acagaagccg
     9781 aaatactggg attaatccgc cgcgtcgccg gaatcagcca gcaggttgac gaacaggcca
     9841 tgcaaccgga cagcgtgacc gctgaaaatt atgcgcgtgt ggtggatgag gtaatgcgcc
     9901 gtgacggtat tgagcttaac ggcgtggata tgcgcaacat acgaaccaga gttcttgagt
     9961 tgctggcata ccgtcgccgt tctcaacaac ggagggagag cgcgaaaaat acttaccagt
    10021 ggaggaagcc ggagcactta cggcggtaac tggttgatat taccgaaagt gcaaaaatgc
    10081 acttagcaac tggtggcgag ttgcagatct gcaactcgac tatgaaacta cggaaactac
    10141 ccgtagtttg ggtagtaaga gtaacaccca gattttgggg cttactcgcg atacccaaat
    10201 aaagggtatc ggtggaagaa atatcgtttc tcatatgtga gtaccgaggg cggaattccg
    10261 ccttcggtta cttattgtgc gcatgcacag ggaggggcgg gtcaaatctc tgtaccctga
    10321 cgtcttccgg actgccagcc ccatcgattt tttatacccg cgaaaaatga aaaacgcttc
    10381 acgctggtgg gcctgatgcc gataactggt ttcatgactg atagccggaa aatgataacg
    10441 attaaagcgt gttgatcttt gttcgtggtt gttcgatatt gttcgtggaa cggtgtagtt
    10501 aatggtgtag ttaattttac tgtttttgac gagcttattg tttgcaactc aatgaaaaat
    10561 aaaggctaat aagccaaatg ctataaatga tagttatcta tcatgtggag tagattggtc
    10621 aggcaaataa gctcttgtca gcggcagggc gttctgccga taaccgtaac cgaagatgat
    10681 aactgacaat gggtaaaacg aacgactggc tggactttga tcaactggcg gaagaaaaag
    10741 ttcgcgacgc gctaaaaccg ccatctatgt ataaagtgat attagtcaat gatgattaca
    10801 ctccgatgga gtttgttatt gacgtgttac aaaaattctt ttcttatgat gtagaacgtg
    10861 caacgcaatt gatgctcgct gttcactacc aggggaaggc catttgcgga gtctttaccg
    10921 ccgaggttgc agaaaccaaa gtggcgatgg tgaacaagta cgcgagggag aatgagcatc
    10981 cattgctgtg tacgctagaa aaagcctgaa tgcaggcata aaaattgggg gaggtgccta
    11041 tgctcaatca agaactggaa ctcagtttaa atatggcttt cgccagagcg cgcgagcacc
    11101 gtcatgagtt tatgaccgtc gagcacttgt tactggcgct gctcagtaac ccatctgccc
    11161 gggaggcgct ggaagcgtgt tctgtggatt tggttgcgct ccgtcaggaa ctggaagcct
    11221 ttattgaaca aaccacaccc gttctgcctg ccagtgaaga ggagcgcgac acacagccga
    11281 cgctgagttt tcagcgtgta ctgcaacgtg cggtcttcca tgtccagtcc tccggtcgca
    11341 atgaggttac cggtgcaaac gttctggtcg ctatctttag cgaacaggag tcgcaggcgg
    11401 catatctgtt gcgtaaacat gaagtcagcc gtctcgatgt ggtgaatttt atctctcatg
    11461 gcacgcgtaa agacgagccg acacagtctt ctgatcctgg cagccagcca aacagcgaag
    11521 aacaagctgg tggggaggaa cgtatggaga atttcacgac gaacctgaat cagcttgcgc
    11581 gcgtgggcgg aatcgaccca ctgattggtc gtgagaagga gctggagcgt gctattcagg
    11641 ttctctgccg tcgccgtaaa aacaacccgc tgctggtggg ggaatctggt gtcggtaaaa
    11701 ccgcgattgc ggaaggtctt gcctggcgaa ttgttcaggg cgatgtgccg gaagtgatgg
    11761 ctgactgtac gatttactct ctcgatatcg gttctctgtt agcgggcaca aaatatcgcg
    11821 gcgactttga aaaacgtttt aaagcgttgc tcaagcagct ggagcaggac actaacagca
    11881 tcctgtttat tgatgagatc cacaccatta tcggtgcggg ggcagcgtct ggtggtcagg
    11941 ttgatgcggc taacctaatc aaaccgttgc tctccagcgg taaaattcgt gtaattggtt
    12001 cgacaaccta tcaggagttc agcaacattt tcgagaaaga ccgtgctctg gcgcgtcgct
    12061 tccagaaaat tgatattact gaaccgtcaa tcgaagaaac tgttcaaatc atcaatggcc
    12121 tgaaaccgaa gtatgaagcg caccacgacg tgcgttatac cgcaaaagcg gtgcgtgcgg
    12181 cggtagagct ggcggtgaaa tacatcaatg atcgtcatct gccggataaa gcgattgatg
    12241 ttatcgacga agcgggcgct cgcgcacgcc tgatgccggt aagcaaacgc aagaaaaccg
    12301 ttaatgtggc ggatattgag tccgtggtgg cccgtattgc acgcattcca gagaagagtg
    12361 tttctcagag tgaccgcgat accctgaaaa acctcggcga tcgcctgaaa atgctggtct
    12421 tcggtcagga taaagccatt gaggcgctga ctgaagccat taagatggcg cgtgcaggtt
    12481 taggtcacga acataaaccg gtcggttcgt tcctgtttgc cggtcctacc ggggtcggga
    12541 aaacagaggt gacggtacag ctttcgaaag cgttgggcat tgagcttctg cgctttgata
    12601 tgtccgagta tatggaacgc cataccgtca gccgtctgat tggtgcgcct ccgggatacg
    12661 ttggttttga tcagggcggt ttgctgaccg atgcggtaat caagcatcca catgcggtgc
    12721 tgctgctgga cgaaatcgag aaagcgcatc cggacgtgtt caatattctg ttgcaggtga
    12781 tggacaacgg tacgctgacc gataacaacg gacgcaaagc ggacttccgt aacgtggtgc
    12841 tggtgatgac caccaacgcc ggggtacggg aaactgagcg caaatccatt ggtcttatcc
    12901 accaggataa cagcaccgat gcgatggaag agatcaagaa gatctttaca ccggaattcc
    12961 gtaaccgtct cgacaacatt atctggtttg atcatctgtc aaccgacgtg atccatcagg
    13021 tggtggataa attcatcgtc gagttgcagg ttcagctgga tcagaaaggt gtttctctgg
    13081 aagtgagcca ggaagcgcgt aactggctgg ccgagaaagg ttacgaccgg gcaatgggcg
    13141 ctcgtccgat ggcgcgtgtc atccaggaca acctgaaaaa accgctcgcc aacgaactgc
    13201 tgtttggttc gctggtggac ggcggtcagg tcaccgtcgc gctggataaa gagaaaaatg
    13261 agctgactta cggattccag agtgcacaaa agcacaaggc ggaagcagcg cattaatcta
    13321 attgttaggt aggttggtga agtccgtaat ctcgaaagag gttacggact ttttgtttat
    13381 ggggtggaac ggtgaaaacc ctatttttgg aggtgaaggt aatttgttga taattagtgc
    13441 tgctggaaaa ggtaaggata aaaaagggtg acagcaggaa aatgagatgg ttttgcttta
    13501 ttaacaacgg actaaacgtg tagtatttga gttcactgcc gtacaggcag cttagaaatc
    13561 gacggggtgc ggtaaaacct ttgcgaacgc gttcactgcc gtacaggcag cttagaaatt
    13621 cacaggtaac atattccacc cgcccaccat gttcactgcc gtacagaaag ataaaatgcg
    13681 aaaaaaaagc tcgcactttc gtacgagctc ttctttaaat atggcggtga gggggggatt
    13741 cgaacccccg atacgttgcc gtatacacac tttccaggcg tgctccttca gccactcgga
    13801 cacctcacca aattgttttg ctaccaaacc tcatgggtgg caacggggcg ctactatagg
    13861 gagttggagt aaaacggtca agaagaattt taatgataat tattgtttgc tcatactgta
    13921 aacaagttgt gcagtatatc tacatcgaga caagttacgg acttatactt ccaaagtact
    13981 tcatacatat cacaaaataa aaaggccggt taaaccgacc ttttactcgt tctttctctt
    14041 cgcccatcag gcggtaaaac aatcagcgac tacggaagac aatgcggcct ttgctcaggt
    14101 cgtacggggt cagttcaaca gtcactttgt cgcccgtcag gatgcggatg tagtttttgc
    14161 gcattttacc ggagatgtgt gcagtaacca cgtgaccgtt ttctaactct acgcggaaca
    14221 tggtattagg caacgtttca agaacggtac cttgcatttc aatattgtct tctttggcca
    14281 tctaatcctc tggggtatca ctaccgtaat ttgaaccggc aagataatgc cgaagttctg
    14341 taaataagta aagatttgcg cgctaaatcg caacaaacag gttcggcaca ttactccgaa
    14401 aacacacggc taagccgcac caaaagcgca acgtataagg gagcggtgag ataaacgatg
    14461 ggcgttacct gacgcgaaaa attccttatc ggcagcgggg taatgagcat aaccaactct
    14521 gcgaccgcaa ttataacact ctggggagaa atgtgccgaa aacattcatt tttgtggtga
    14581 aaacaagcat cgtggcaccc agaaattatt cggcaatcgt ccgaggcgca tttgattgag
    14641 ataattcaga taatcccgac ggggaatttc gcaggcacca agcgatgctg tgtgatcgtt
    14701 aaggacctgg cagtcgataa gcttaccgcc atgactgata aattcctcac agaataccag
    14761 aagcgccgtt ttagacgcat tttccatccg gctgaacatg gactcgccac aaaatagcgt
    14821 tccctgggcc acgccgtaca taccgccgac aagctcatct tcatgccaga cttcaatgga
    14881 gtgggcatga ccgagttcgt gaaggcgatg gtaggcttcg accacgccac gcgtgatcca
    14941 ggttccttct tcgcgatcgc tggcacaacc ttcaatgacc tgaccaaaag cgtaattcat
    15001 cgtgacacga tagggcgagc gtttatgaaa tcgcttcata ctacggctga tatgcagtga
    15061 ttcaggccaa agcaccgcgc gaggatcggg cgaccaccag aggatggggg cgcctggaga
    15121 aaaccacgga aaaataccac gctggtaagc cattaacagg cgcgcagggc taagatcgcc
    15181 ccccagagcc agcaggccgt taggctcacg taatgcgcct tccggggaag ggaaggctat
    15241 tgaatggcga gaaagctgaa ccaggcgcat gaccgcaaaa ctccacgcaa gtcggatcgt
    15301 tcaataatag cttacaaacc ctgcttaaac tggtaataac gcccctgtct ggcaagcagt
    15361 tctgcgtgag taccttgctc aataatttgc ccgttgtcca tcactattat ttgttggaaa
    15421 cgagagagtc cgcgaagtcg atgggtgacc attaacaccg ttttctcacg catcatttct
    15481 gcaagcaatt caaggatctg gctttcggtt gtggcatcta agccttcggt aggttcatcc
    15541 agcaacacca gtggcgcatc atgtaacagc gcacgggcga tagccagacg gcgcagttca
    15601 ccaccggaga gctggcgtcc gccttcacct aaccaactgt tgagacctgc gtcctcgagc
    15661 agcttctcca gaccaacgcg acgcaagatc tccgacagag cctcatcact actgccaggc
    15721 gaggcgagta acagattatc acgcagcgtg gcgctaaaca gatgcactcg ctgaggaaca
    15781 acgctgatgg tctgtcgtag agccgcttca ttcaggctgg ctatggggct atcgttaagc
    15841 aaaatctcgc cctgttgcgg gtcccaagcg cgggtcagca gttgtaacag tgttgatttg
    15901 ccgcatccgg ttcgcccgag aatcgcaata tgttccccgg cgtttacctg aagagaaatc
    15961 cctttaagtg cctgttggga ttgctccgga taagtgaact gaacatcccg taatgtcagc
    16021 gaaacgcgat cggcaacacg agtttgggta tcagggaagg tgacttccgg tttttgatcc
    16081 gttaaatcag tgatccgaac ggcagaggca atgacttgcc ccagatgctg aaatgcaccc
    16141 gttactggtg ccagtgcttc aaacgcggct aacgcgcaga agacaaacag ggcaattaac
    16201 gcgccgggtt gagcattgcc gccaacgccg ccagacgcca tccacagcat caggatcacc
    16261 gctaacgcgc caatgagcag cattatcgct tgcgacaatg cggtcagttc agattgacgg
    16321 cgttgcgctt ccagccattg aatttctgta ttctctagtt gcgtgcgata acgatcgctg
    16381 gcaccaaaaa tggtcagctc agcttgccct tgcagccagg ccgtcagttg ttggcgatac
    16441 tgtccgcgaa gatgagtcag attttgcccg gtgctttttc ccgcacgata aaacagcggt
    16501 ggcatcagga aaagcgtcag taacataatg ccgcccagcg taaaggcgag ggtgaaatca
    16561 aggaaactta acccgactgt caccaccata atcaccacaa aagcgcccac cagcggcgag
    16621 ataacgcgca ggtaaagatg atcgagcgta tcaacatccg ccaccacgcg attgagcaat
    16681 tcgccctgac gatagcgcgc cagtccggca ggggagaggg gcaacaattt gctgaaggtg
    16741 taaatgcgca gatgctgcaa cacgcggaaa gtcgcgtcgt gacttaccag acgttcaaaa
    16801 taacgcccgg cagtacgggt gattgcggca ccacgcacgc ccgcagcggg tagcatatag
    16861 ttgaagctgt acagtccggc aacccccgca accgctgagg ccgagaggaa ccagccggaa
    16921 agtgtcaaca gaccgatact ggcgagcagc gtcacaattg ccagcacaat accaagactt
    16981 aacatccatt tatgacgttt atacagtgcc agatagggta gcaaagcgcg catttaaatc
    17041 tcctcctgac gatgggccag taatgtggcg aatgggccac cagccacact taattccgcg
    17101 taacgtcctt gctcaataat ctgaccatcc tgcattaccc aaatgacatc ccagtcagca
    17161 agatcttcta actggtgggt gaccattaac gttgtctggc gcagagaggc ggcattcagc
    17221 gcctccatta cgcgctgttc actgtgagca tcaaggctgg cagcgggttc atccaacagt
    17281 aatagcgaac agggatttag taacgcacgg gccaccgcca cgcgctgcgc ctgccccacg
    17341 gaaaggcggg cagcctggtc gccaacaggc gtatcaacgc cttgtggcag gagcggtaga
    17401 aactcgctga cccaggcgtt atccagcgct gcttgtaatt catgttcgct ggcatcaggt
    17461 cgcgccagta gtacgttatc ccgtaatgtt gctgccggta attgtggatt ttgcccaacc
    17521 caggagagat gtttacgcca tgattctggt gataaatcgc gtaattctat cccgttgatt
    17581 cgtagcgatc cctgatatga gagaaaacca gaaagcgcgt tcaacagtga acttttacct
    17641 gaaccgctgc gaccaaccaa cacggcacgt tggcctgctg gcaaagtaaa gttcagcggt
    17701 ccggccagcg ttttaccttc cggcgacgtg ataaacagat cctcggcctc aatggtcacc
    17761 ggatcggtcg atgctaattc cacctcgcca cgctgcggat gggcgagcgg ggtttccata
    17821 aacgttttca gactgtcagc tgcaccaaca gcctgggctt tagcatgata aaacgtaccg
    17881 agatcgcgta atggctggaa aaactctggc gcaaggatca gggccagaaa acccgcagcc
    17941 agcgtcacgc cagtatcgta gtgaccaaaa tccagctcgc cgagatagga aaaaccaaag
    18001 tagaccgcca ccagagcaat tgacagcgag gtaaaaaatt cgagaatgcc ggaggataaa
    18061 aatgccagcc gtagcacttc cattgtccgt tggcggaaat cttccgaagc agaacgaata
    18121 ctttcaattt cagcttcacc acgaccaaaa atacgcaatg tttccatgcc gcgcaggcga
    18181 tcgaggaaat gcccacttaa gcgagcaaga gcgagaaagt tacgtcggtt agcatcggca
    18241 gcccccattc caaccagcgc cataaacaac ggaattaacg gtgcagtgcc cagcagaatg
    18301 agcgccgcag cccagtttga ggggaagatt gccaccacaa tcagcaacgg caccgacact
    18361 gccagcgcca tttgcggcag gtagcgtgca tagtaatcat gcatatcgtc aatttgctcg
    18421 agtaccagcg tcgcccagct ccccgcaggt ttaccctgaa tccacgctgg ccctgcttgt
    18481 tgcagacggt cgagaacctg acggcggatg gcaaagcgga tatgctgccc ggcgtgataa
    18541 cccacccgtt cgcgtaacca gaccgcccat gcgcgcagta caaaggtcag aaccagtaac
    18601 gtaaagggaa gcagcagggc ttcacgggga atattctcca taatcatatg ttgcagaatt
    18661 cgcgccatga accaggcctg ggcaatgatc aatatgccgc ttacaaagcc cagcagacga
    18721 gaaatattca gccaacgttg ggagatgacg ctttgctgtt ttaaccagcg ggttaactct
    18781 ttttgacgag atttattcat tgcacgctta gcaggtgagt tatcagaatt atttgcagag
    18841 caatgttaca acggggaaaa aataaaggcg acccatagtc gcaaggtgtc gccttcttta
    18901 cttttgttac tgatttgtaa aattattttg cgtcagctaa accatcgagg tagcgttccg
    18961 catcaagtgc tgccatgcag ccagtaccgg ccgaagtaat ggcctggcga taaatgtgat
    19021 ccatcacgtc gcctgcggca aagacgccag gaatgctggt ctgggtggca ttaccatgaa
    19081 tacccgactg tactttgatg tagccgtttt ccagttccag ctgcccttcg aaaatcgcag
    19141 tattcgggct gtgaccgata gcaacaaaca gaccggcaac gtcgagtgac tcgatgttat
    19201 cgctgttttg cgtatcgcgc agacgaacgc cagtgacacc catttgatcg ccggtcactt
    19261 cttccagcgt acggttggtg tgcagaatga tgttgccgtt ctccacttta tccatcagac
    19321 gcttaataag gattttttcc gcgcggaaac cgtcacggcg gtgaatcaga tgcacttccg
    19381 aagcgatgtt agacagatac agcgcctctt caaccgcggt attgccgccg ccgatgaccg
    19441 caactttctg gttgcgatag aagaaaccgt cgcaggttgc acaagcagaa accccacggc
    19501 ctttaaaggc ttcttcagag ggcaggccga gatagcgtgc agaagctccg gtggcaataa
    19561 tcagcgcgtc gcaagtgtat tcgccgttat cgccattcag acggaacgga cggttttgca
    19621 gatccacctt gttgatatga tcaaaaatga tctcagtttc aaacttggtg gcatgttcgt
    19681 gcatgcgctc cattaataac ggaccggtca gatcgtttgg atcaccaggc cagttttcca
    19741 cttccgtggt ggtggtcagt tggccgcctt tttccatgcc ggtaatcagc acaggttgca
    19801 ggttggcgcg cgccgcgtag acagcagcgg tgtatcccgc cgggcctgaa cccaggataa
    19861 gcagtttact gtgtttggtc gtgcccatga gatccccata gttgttggca gacaatgggc
    19921 aggattgtag ggaatttaca gacgtaaaaa aagagtatga cgattttgtt aacaatttgt
    19981 gcaatcggca gcatcgataa gcaggtcaaa ttctcccgtc attatcacct ctgctactta
    20041 aatttcccgc tttataagcc gattaaatga tgaataaacg cccctgttaa tgaatatctg
    20101 gcatgttgta ctaaaaatcg atgttttgct ttgacaatcc cctggtgttt tgcgaaaaca
    20161 ttcgaggaag aaaaaaaaca gtattcttat atgcgcataa ccatgcatgt aaataccatg
    20221 tttaccgtgc tagtgaaatc tacgtatggc gtggacagac gccattcgtg atgtcgatag
    20281 ctgccacaag gcaacggtct tctcaccgta gacccaggca ttgcgcgccg tgaatcttca
    20341 tgatttcggt ctatcgtgac gggtagcgac tctgaacagt gatgtttcag gggcagacag
    20401 gagtagggaa ggaatacaga gagacaataa taatggtaga tagcaagaag cgccttggca
    20461 aagatctcga ccgtatcgat cgtaacattc ttaatgagtt gcaaaaggat gggcgtattt
    20521 ctaacgtcga gctttctaaa cgtgtgggac tttccccaac gccgtgcctt gagcgtgtgc
    20581 gtcggctgga aagacaaggg tttattcagg gctatacggc gctgcttaac ccccattatc
    20641 tggatgcatc acttctggta ttcgttgaga ttactctgaa tcgtggcgca ccggatgtgt
    20701 ttgaacaatt caataccgct gtacaaaaac ttgaagaaat tcaggagtgt catttagtat
    20761 ctggtgattt cgactacctg ttgaaaacac gcgtgccgga tatgtcagct taccgtaagt
    20821 tgctggggga aaccctgctg cgtctgcctg gcgtcaatga cacacggaca tacgttgtta
    20881 tggaagaagt caagcagagt aatcgtctgg ttattaagac gcgctaacac ggaacaggtg
    20941 caaaatcggc gtattttgat tacactcctg ttaatccata cagcaacagt actggggtaa
    21001 cctggtactg ttgtccgttt tagcatcggg caggaaaagc ctgtaacctg gagagccttt
    21061 cttgagccag gaatacactg aagacaaaga agtcacattg acaaagttaa gtagcggccg
    21121 ccgccttctg gaggcgttgc tgatccttat tgtcctgttt gccgtctggt tgatggctgc
    21181 cttactaagc tttaaccctt cggaccccag ctggtcgcaa acggcctggc atgaacctat
    21241 ccataattta ggtgggatgc ccggtgcgtg gttggcagat acgctgttct ttatttttgg
    21301 cgtgatggct tacaccattc ccgtcattat tgtcggcggt tgttggtttg cctggcgtca
    21361 tcagtccagc gacgaataca ttgattattt tgccgtttcg ctacgcatca ttggcgtttt
    21421 ggcgctcatc cttacctcct gtggtctggc ggcaatcaac gctgacgata tctggtattt
    21481 tgcctccggt ggcgtcattg gcagcttact aagcactacg ctacaaccac tgctacacag
    21541 tagcggggga actattgcgc tgctctgcgt ttgggcagcg ggcctgacgt tgttcaccgg
    21601 ttggtcatgg gtgaccattg ctgaaaaact cggcggctgg attttaaaca ttctcacctt
    21661 cgccagtaat cgtacccgtc gcgatgatac ctgggtcgat gaagatgagt atgaagacga
    21721 cgaagagtat gaagatgaaa atcacggcaa acagcatgaa tcacgccgtg cccgtattct
    21781 tcgcggcgcg ctagcgcgtc gtaaacggtt ggcggaaaaa ttcattaatc cgatggggcg
    21841 gcaaacagac gctgcgttgt tctccggtaa gcggatggat gatgacgaag agattaccta
    21901 cactgcacgc ggtgtggctg ccgaccctga cgacgtccta ttttcgggca atcgtgcaac
    21961 gcagccagaa tatgacgaat acgatccatt attaaacggt gcgccaatta ccgaacctgt
    22021 cgctgtagca gctgctgcta ccacggcggc acaaagctgg gctgcgccgg ttgaacctgt
    22081 gactcagacg ccgcctgttg cctctgttga tgttccacct tcgcaaccta cagtagcctg
    22141 gcagcctgta ccgggtccac aaacgggaga gccggttatt gctcctgcac cggaaggtta
    22201 cccacagcag ccacaatatg cgcagcctgc agtgcaatat aatgagccgc tgcaacaacc
    22261 agtacagcca cagcagccgt attatgcacc tgcagctgaa caacctgtgc aacaacctta
    22321 ctacgctcct gcggcggaac aacctgttca gcaaccgtat tacgcccctg cgccagaaca
    22381 accggtggca ggtaacgcct ggcaagccga agagcagcaa tccacttttg ctccacagtc
    22441 tacataccag actgagcaaa cttatcagca accagccgct caggagccgt tgtaccaaca
    22501 gccgcaaccc gttgaacagc agcctgttgt ggagcctgaa cccgttgtag aagagacaaa
    22561 acccgcgcgt ccgccacttt actattttga agaagtggaa gagaagcgag cccgtgaacg
    22621 tgaacaactt gcggcctggt atcaaccgat tccagaaccg gttaaagaac cagaaccgat
    22681 caaatcttcg ctgaaagcac cttctgttgc agcagtacct ccagtagaaa ccgctgccgc
    22741 tgtttctccg ctggcatctg gcgtgaaaaa agcgacactg gcgacggggg ctgccgcaac
    22801 cgttgccgcg ccagtcttca gtctggcaaa tagcggtgga ccgcgtccgc aggtcaaaga
    22861 ggggattggc ccgcagttgc cacgaccgaa acgtatccgc gtgccaactc gccgtgaatt
    22921 ggcgtcttac ggtattaagc tgccctcaca gcgtgcggcg gaagaaaaag cccgtgaagc
    22981 ccagcgcaat cagtacgatt ctggcgatca gtacaacgat gatgaaatcg atgcgatgca
    23041 gcaggatgaa ctggcacgtc agttcgccca gacacagcag caacgctatg gcgaacagta
    23101 tcaacatgat gtgcccgtaa acgcagaaga tgcagatgct gcggcagagg ctgaactggc
    23161 tcgtcagttt gcgcaaactc aacaacaacg ttattccggc gaacaaccgg ctggggcgaa
    23221 tccgttctcg ctggatgatt ttgaattttc gccaatgaaa gcgttgctgg atgatggtcc
    23281 acacgaaccg ttgtttacgc caattgttga acctgtacag cagccgcaac aaccggttgc
    23341 accgcagcag cagtatcagc agccacaaca gccggttgcg ccacagcagc aatatcagca
    23401 gccgcaacag ccggttgcgc cgcagccaca atatcagcag ccgcaacaac cggttgcgcc
    23461 acagcagcaa tatcagcagc cgcaacaacc agttgcgccg cagccgcagt atcaacagcc
    23521 gcaacaaccg gttgcgccgc agcagcagta tcagcagcca caacagccag ttgcgccaca
    23581 accgcaggat accctgcttc atccgctgtt gatgcgtaat ggcgacagcc gtccgttgca
    23641 taaaccgacg acgccgctgc cttctctgga tttgctgaca ccgccgccga gcgaagtgga
    23701 gccggtagat acctttgcgc ttgaacaaat ggctcgcctg gtggaagcgc gtctggctga
    23761 tttccgtatt aaagccgatg ttgtcaatta ctctccgggg ccggttatca ctcgctttga
    23821 attgaacctg gcaccgggcg taaaagcggc gcgcatttct aacttgtcac gggaccttgc
    23881 ccgttcactt tcgacggtgg cggtgcgtgt cgttgaagtt attcctggca aaccctatgt
    23941 aggtctggag ttaccgaata aaaaacgaca aaccgtttat ctgcgcgaag ttttggataa
    24001 cgccaaattc cgcgataatc cgtcgccatt aaccgtggtg ctgggtaaag atatcgccgg
    24061 tgagccggtg gttgccgatc tggcgaaaat gccgcacttg ttggttgcgg ggactaccgg
    24121 ttccggtaaa tctgtcggtg tgaacgcgat gatcctgagc atgctttata aagcacagcc
    24181 agaagatgtg cgtttcatca tgatcgaccc gaaaatgctg gagctttcgg tttatgaagg
    24241 cattccgcat ctgttaacgg aagtcgttac tgatatgaaa gatgccgcca acgcgctgcg
    24301 ctggtgtgtt aacgagatgg agcgtcgtta taaactgatg tctgcgctgg gtgtgcgtaa
    24361 tctggcgggt tataacgaaa aaattgctga agccgatcgc atgatgcgtc cgattccaga
    24421 cccgtactgg aagccgggtg acagtatgga tgcccagcat ccggtgctga aaaaagaacc
    24481 atacattgtg gtgttggttg acgaatttgc cgacctgatg atgacggtag gtaaaaaagt
    24541 ggaagagctg atagcacgtc tggcgcaaaa agcccgtgcc gcgggtatcc acctcgtact
    24601 ggcaactcag cgtccatcgg ttgatgttat tactggtctg attaaagcga atattccgac
    24661 ccgtatcgcc tttaccgtat ccagtaagat tgactcacgt accattcttg atcaggctgg
    24721 cgcggaatca ctgctgggga tgggggatat gctctactct gggccgaact ccacgttgcc
    24781 ggtacgtgtc catggtgctt ttgttcgcga tcaggaagtt catgccgtgg tgcaggactg
    24841 gaaagcgcgt ggtcgcccac agtatgttga tggcatcact tccgacagtg aaagcgaagg
    24901 tggcgcgggc ggtttcgatg gcgctgaaga actggatccg ttgttcgatc aggcggtgca
    24961 gtttgtcact gaaaaacgca aagcgtcaat ttctggcgta cagcgtcagt tccgcattgg
    25021 ttataaccgt gcagcgcgta ttatcgaaca gatggaagcg caggggattg tcagcgaaca
    25081 ggggcacaac ggtaatcgtg aagtgctggc tccaccgccg tttgactaat taatgcattg
    25141 ccggataagg cgcggtagcg tcgcatccgg cactctatca actgaaaatt cagtattttc
    25201 ttctttcctc aagctgatta ttagcctgga atagagagta gagggaactc ccgatcggga
    25261 gtgacgtaat ttgaggaata atgatgaaaa aaattgccat cacctgtgca ttactctcaa
    25321 gcttagtagc aagcagcgtt tgggctgatg ccgcaagcga tctgaaaagc cgcctggata
    25381 aagtcagcag cttccacgcc agcttcacac aaaaagtgac tgacggtagc ggcgcggcgg
    25441 tgcaggaagg tcagggcgat ctgtgggtga aacgtccaaa cttattcaac tggcatatga
    25501 cacaacctga tgaaagcatt ctggtttctg acggtaaaac actgtggttc tataacccgt
    25561 tcgttgagca agctacggca acttggctga aagatgccac cggtaatacg ccgtttatgc
    25621 tgattgcccg caaccagtcc agcgactggc agcagtacaa tatcaaacag aatggcgatg
    25681 actttgtcct gacgccgaaa gccagcaatg gcaatctgaa gcagttcacc attaacgtgg
    25741 gacgtgatgg cacaatccat cagtttagcg cggtggagca ggacgatcag cgcagcagtt
    25801 atcaactgaa atcccagcaa aatggggctg tggatgcagc gaaatttacc ttcaccccgc
    25861 cgcaaggcgt cacggtagat gatcaacgta agtagaggca cctgagtgag caatctgtcg
    25921 ctcgattttt cggataatac ttttcaacct ctggccgcgc gtatgcggcc agaaaattta
    25981 gcacagtata tcggccagca acatttgctg gctgcgggga agccgttgcc gcgcgctatc
    26041 gaagccgggc atctgcattc tatgatcctc tgggggccac cgggtaccgg caaaacaacc
    26101 ctcgctgaag tgattgcccg ctatgcgaac gctgatgtgg aacgtatttc tgccgtcacc
    26161 tctggcgtga aagagattcg cgaggcgatc gagcgcgccc ggcaaaaccg caatgcaggt
    26221 cgccgcacta ttctttttgt tgacgaagtg caccgattca acaaaagcca gcaggatgca
    26281 tttctgccac atattgaaga cggcaccatc acttttattg gcgcaaccac tgaaaacccg
    26341 tcgtttgagc ttaattcggc actgctttcc cgtgcccgtg tctatctgtt gaaatccctg
    26401 ggtacagagg atattgagca agtactaact caggcgatgg aagacaagac ccgtggttat
    26461 ggtggtcagg atattgttct gccagatgaa acacgacgcg ccattgctga actggtgaat
    26521 ggcgacgcgc gccgggcgtt aaatacgctg gaaatgatgg cggatatggc cgaagtcaat
    26581 gatagcggta agcgggtcct gaagcctgaa ttattgaccg aaatcgccgg tgagcgaagc
    26641 gcccgctttg ataacaaagg cgatcgcttt tacgatctca tttccgcact gcataagtcg
    26701 gtacgtggta gcgcacccga tgcggcgctc tactggtatg cgcgaattat taccgctggt
    26761 ggcgatccgt tatatgtcgc gcgtcgttgc ctggcaattg cttccgaaga tgtcggcaat
    26821 gccgacccac gggcgatgca ggtggcaatt gcggcctggg attgctttac tcgcgttggc
    26881 ccggcggaag gtgaacgcgc cattgctcag gcgattgttt acctggcctg cgcgccaaaa
    26941 agcaacgctg tctacactgc gtttaaagct gcgctggcgg atgctcgcga acgcccggat
    27001 tatgacgtgc cggttcattt gcgtaatgcg ccgacgaaat taatgaagga aatgggctac
    27061 gggcaggaat atcgttacgc tcatgatgaa gcaaacgctt atgctgccgg tgaggtttac
    27121 ttcccgccgg aaatagcaca aacacgctat tatttcccga caaacagggg ccttgaaggc
    27181 aagattggcg aaaagctcgc ctggctggct gaacaggatc aaaatagccc cataaaacgc
    27241 taccgttaat gttatcgttg cggtaatgtt gttactgtat ccctgtggtc gcaggctgtg
    27301 gccacatctc ccatttaatt cgataagcac aggataagca tgctcgatcc caatctgctg
    27361 cgtaatgagc cagacgcagt cgctgaaaaa ctggcacgcc ggggctttaa gctggatgta
    27421 gataagctgg gcgctcttga agagcgtcgt aaagtattgc aggtcaaaac ggaaaacctg
    27481 caagcggagc gtaactcccg atcgaaatcc attggccagg cgaaagcgcg cggggaagat
    27541 atcgagcctt tacgtctgga agtgaacaaa ctgggcgaag agctggatgc agcaaaagcc
    27601 gagctggatg ctttacaggc tgaaattcgc gatatcgcgc tgaccatccc taacctgcct
    27661 gcagatgaag tgccggtagg taaagacgaa aatgacaacg ttgaagtcag ccgctggggt
    27721 accccgcgtg agtttgactt tgaagttcgt gaccacgtga cgctgggtga aatgcactct
    27781 ggcctcgact ttgcagctgc agttaagctg actggttccc gctttgtggt aatgaaaggg
    27841 cagattgctc gcatgcaccg cgcactgtcg cagtttatgc tggatctgca taccgaacag
    27901 catggctaca gtgagaacta tgttccgtac ctggttaacc aggacacgct gtacggtacg
    27961 ggtcagctgc cgaaatttgc tggcgatctg ttccatactc gtccgctgga agaagaagca
    28021 gacaccagta actatgcgct gatcccaacg gcagaagttc cgctgactaa cctggtgcgc
    28081 ggtgaaatca tcgatgaaga tgatctgcca attaagatga ccgcccacac cccatgcttc
    28141 cgttctgaag ccggttcata tggtcgtgac acccgtggtc tgatccgtat gcaccagttc
    28201 gacaaagttg aaatggtgca gatcgtgcgc ccggaagact caatggcggc gctggaagag
    28261 atgactggtc atgcagaaaa agtcctgcag ttgctgggcc tgccgtaccg taaaatcatc
    28321 ctttgcactg gcgacatggg ctttggcgct tgcaaaactt acgacctgga agtatggatc
    28381 ccggcacaga acacctaccg tgagatctct tcctgctcca acgtttggga tttccaggca
    28441 cgtcgtatgc aggcacgttg ccgcagcaag tcggacaaga aaacccgtct ggttcatacc
    28501 ctgaacggtt ctggtctggc tgttggtcgt acgctggttg cagtaatgga aaactatcag
    28561 caggctgatg gtcgtattga agtaccagaa gttctgcgtc cgtatatgaa cggactggaa
    28621 tatattggct aatacccaat ttttctgaat ctaaaaagcg cctgcgggcg ctttttttgt
    28681 ctccctttga taccgaacaa taattactcc tcacttacac gtaatactac tttcgagtga
    28741 aaatctacct atctctttga ttttcaaatt attcgatgta tacaagccta tatagcgaac
    28801 tgctatagaa ataattacac aatacggttt gttactggaa tcaatcgtga gcaagcttga
    28861 gtgagccatt atgaaaacga aaatccctga tgcggtattg gctgctgagg tgagtcgccg
    28921 tggtttggta aaaacgacag cgatcggcgg cctggcaatg gccagcagcg cattaacatt
    28981 accttttagt cggattgcgc acgctgtcga tagcgccatt ccaacaaaat cagacgaaaa
    29041 ggttatctgg agcgcctgta cagttaactg tggtagtcgc tgcccgctac gtatgcacgt
    29101 cgtggacggt gaaatcaaat atgtcgaaac ggacaatacc ggcgatgaca attacgacgg
    29161 cctgcaccag gttcgcgcct gcctgcgtgg gcgttccatg cgtcgccgtg tctacaatcc
    29221 ggaccgcctg aaatatccga tgaaacgagt cggggcgcgc ggtgaaggca aattcgagcg
    29281 cattagctgg gaagaagcct acgacatcat cgcgaccaat atgcagcgcc tgatcaaaga
    29341 gtacggcaac gagtctatct atctgaacta tggcaccggt acgctgggcg gcaccatgac
    29401 ccgctcctgg ccgccgggaa ataccctggt cgcgcggctg atgaactgct gcggcggcta
    29461 tctgaaccat tacggcgact actcctccgc gcaaattgcg gaaggtttga actataccta
    29521 cggcggctgg gcagatggca acagcccgtc ggatatcgaa aacagtaagc tggtagtgct
    29581 gtttggtaat aaccctggcg aaacgcgaat gagtggcggt ggggtgactt actatcttga
    29641 acaggcacgc cagaaatcta atgcccgcat gatcatcatc gatccgcgct ataccgacac
    29701 cggtgccggg cgcgaagatg agtggatccc tattcgtccg ggaacagatg ccgcactggt
    29761 taacggtctg gcgtacgtca tgatcactga aaacctggtg gatcaggcat tcctcgataa
    29821 atattgcgtt ggctacgatg agaaaaccct gccagccagt gcgccgaaaa atggccacta
    29881 taaagcttat attctgggtg aagggccaga tggcgtggct aaaacaccgg aatgggcctc
    29941 acaaatcacc ggtgttccgg cagacaaaat catcaaactg gctcgtgaaa tcggcagtac
    30001 caaaccggcg tttatcagcc agggatgggg cccgcagcgt cacgctaacg gtgaaatcgc
    30061 aacccgtgct atctcgatgc tggcgattct gaccggtaac gttggtatta atggagggaa
    30121 cagcggcgcg cgtgaaggtt catacagctt accgtttgtc cgtatgccga ccttggaaaa
    30181 cccgatccag accagcattt cgatgtttat gtggaccgat gccattgaac gtggcccgga
    30241 aatgacggcg ctgcgtgatg gtgtacgcgg gaaagataag ctggatgtgc cgatcaaaat
    30301 gatctggaac tatgccggta actgcctggt taaccagcat tctgaaatca accgtaccca
    30361 tgaaatcctt caggatgata agaagtgcga gctgattgtg gttatcgact gccacatgac
    30421 ctcatcggcg aaatatgctg acatcctgct gcctgactgc accgcttccg aacagatgga
    30481 ctttgcgctg gatgcatcct gcgggaatat gtcttacgtg attttcaacg atcaggtgat
    30541 taaaccgcgc tttgaatgta agaccatcta tgaaatgacc agcgaactgg caaaacgtct
    30601 tggcgttgag caacagttta ctgaaggccg tacccaggaa gagtggatgc ggcatctgta
    30661 tgcccagtcg cgggaagcga ttcctgaact gccaacgttt gaagagttcc gcaagcaggg
    30721 gatctttaaa aagcgcgacc cacaagggca tcacgttgct tataaagcct tccgtgaaga
    30781 tccgcaggca aacccactga ctacgccatc gggcaaaatt gagatttatt cgcaggcgct
    30841 ggctgacatt gccgctacct gggaattgcc agaaggcgat gtgatcgatc cactgccgat
    30901 ctacacgccg ggctttgaaa gttatcagga tccgctgaac aaacagtatc cgctgcagct
    30961 tacgggtttc cactataaat ctcgcgttca ctcaacttac ggcaacgttg atgtgctgaa
    31021 agcagcttgc cgtcaggaaa tgtggatcaa cccgcttgat gcgcaaaaac gcggtatcaa
    31081 caacggcgat aaagtcagga tctttaacga tcgtggtgaa gttcatattg aggcgaaagt
    31141 gacgccacga atgatgccag gtgtggtcgc actgggtgaa ggtgcctggt atgacccgga
    31201 tgcaaaacgt gtcgataagg gtggttgtat taacgtactg accactcaac gtccgtctcc
    31261 tctcgctaag gggaatccgt cacatacaaa ccttgttcag gttgaaaagg tgtaaggagt
    31321 aaccgatgac aacccagtat ggatttttta ttgattccag ccgttgcacc ggttgcaaaa
    31381 cctgcgagct ggcttgtaaa gactacaaag atttgacgcc agaagtcagc ttccgccgca
    31441 tttatgaata tgctggcggc gactggcagg aagataacgg tgtctggcac cagaacgtgt
    31501 ttgcctacta tctgtcgatt tcgtgcaacc actgcgaaga tccggcttgt actaaagtct
    31561 gcccgagcgg tgcgatgcat aaacgtgaag atggttttgt tgtggtcgat gaagatgtgt
    31621 gcattggctg ccgctactgc catatggctt gcccgtatgg cgcaccgcaa tataacgaaa
    31681 cgaaaggcca tatgaccaaa tgcgatggtt gttatgaccg tgttgcggag ggtaaaaagc
    31741 cgatctgtgt tgaatcttgt ccgctgcggg cgctggattt cggccctatc gacgagctgc
    31801 gtaaaaaaca tggcgatctg gcggccgttg cgccgttgcc gcgagctcac tttaccaaac
    31861 cgaatattgt gatcaaaccc aatgccaata gccgcccgac cggggatacc actggctatc
    31921 tggcaaaccc gaaggaggtg tgagatggga agtggatggc atgaatggcc gctgatgatc
    31981 ttcacggtct tcgggcaatg tgtggcgggt ggttttatcg ttctggcttt ggcgctgctc
    32041 aaaggcgacc tgcgagcaga agcccagcag cgtgttatcg cctgcatgtt tggcttatgg
    32101 gtgctgatgg gcattggctt tatcgcttct atgctccatc ttggttcacc gatgcgcgct
    32161 tttaactcgc tcaaccgggt aggggcttca gcactcagta acgaaatcgc cagcggttcg
    32221 atcttttttg ccgtaggcgg catcggctgg ttgctggcaa tgctgaaaaa gctgtcaccg
    32281 gcattgcgta cgctttggct ggtggtgacg atggttcttg gcgtcgtctt tgtctggatg
    32341 atggtgcgtg tgtataacag cattgatacc gttccgacct ggtacagcat ctggacgccg
    32401 atgggcttct tcctgacgat gttcatgggc ggcccgttac tggggtacct gttgctgagt
    32461 ctggctggcg tcgatagctg ggcgatgcgt ctgctgccag ccatttccgt actggcactg
    32521 gtggtgagtg gcgtggtgtc agtgatgcag ggcgcagagc tggcgactat tcatagttct
    32581 gtgcagcagg ccgcagcgct ggtgccggac tatggtgcgc tgatgtcctg gcggatcgtg
    32641 cttttggccg ttgccctgtg cttgtggatt gcaccacagc taaaaggtta tcagcctgcg
    32701 gttccgctac tatccgtctc attcattctg cttctggcag gggaattaat cggtcgcggc
    32761 gtattctatg gtttgcatat gaccgtgggg atggccgtcg caagctaatc agtaacaacc
    32821 ggggtttcgg ccccggttct cttttatttc tgcttcgtta acgtgtcata actggtcatc
    32881 aaattacgat aatcaggaat atggttagag aacaacgtcg ccagtccttc aatatcatta
    32941 cgccagtcac ggtgcagctc gcaagccacg ccgaaccatg tcatcaattg cgcacctgct
    33001 tgcgacatgc gatcccatgc cgaatgccgg gtaatttcat taaaggtccc tgacgcgtca
    33061 gtcacaacaa acacatcaaa cccttcttca atggctgaaa gcgccgggaa tgccacgcaa
    33121 acttcggtta ccacaccggc aataattaac tgttttttac ctgtcgcttt gactgctttt
    33181 acaaaatctt cgttatccca ggcgttaata ttgcccgggc gagcaatgta aggtgcatcg
    33241 gggaattgtg ctttcagttc tggaactaat gggccgttag gaccggtttc aaaactggtg
    33301 gtaagaatag ttggtaagtt gaaatactta gccaggtcac ccagcgccag cacgttattt
    33361 ttaaacttat cgggttcgat atcccgtaca agggaaagta aaccagcctg gtgatcaaca
    33421 agcaaaacag cagcatcatt tttatcaaga cgaacatacg gtttggtcat cctcttctcc
    33481 tttcgagagt cagtggtacg cctgaaatcg gtcagacgtg atgcattgct ctgaaagcat
    33541 agacgggaaa tatgagtttg ctgtgaccat gaaatttttc gactgaacga agcgtatgaa
    33601 atttgtgtta gatcaataaa aacaatcaga tgagattaat catctcttca gtattagcgt
    33661 tatgaatgag taatataaat cgataatacc gccgttgcga tatgcgcgta ttttcaccca
    33721 ttgacaatgt ttttggcggt ggcatgatgc gcatgaaatt tgaacttcct cacggtttta
    33781 attcatgtcc acgtataccc ggcctgtcat gcttttgctg tctggcctgc ttttgttgac
    33841 tctggcgatc gcggtgttaa atacactcgt gccgctttgg ctcgcccagg aacacatgtc
    33901 cacatggcag gtaggcgttg tcagctcatc ctattttacc ggcaaccttg tcggtacatt
    33961 gctgacaggg tatgtcatta aacgcattgg ctttaaccgc agctattatc tggcctcctt
    34021 catttttgcc gctggctgtg ccggccttgg cctgatgatt ggattctgga gttggttggc
    34081 gtggcgtttt gtcgcgggcg tcggctgtgc catgatttgg gtagtcgttg agagcgcgct
    34141 gatgtgcagt gggacgtcac gtaaccgtgg gcgtttgctt gctgcataca tgatggttta
    34201 ttacgtggga acgtttttag gccagttact ggtcagcaag gtctcaaccg agctgatgag
    34261 cgtattgccg tgggttacag gtttgacatt ggcagggatc ttaccgctgt tgtttacgcg
    34321 tgtcctgaat cagcaggctg aaaaccatga ttcgacgtca attacggcaa tgctaaaact
    34381 ccgtcaggcg cggcttggcg tgaatggctg cattatctca ggaatcgttc tgggatctct
    34441 atatggcctg atgcctctgt acctcaatca caaaggggtg agcaatgcca gtattggttt
    34501 ctggatggcg gtactggtca gtgcgggtat cctcggacaa tggccgattg gacgtctggc
    34561 ggataagttt ggtcgactgc tggttttgcg tgttcaggtc tttgtcgtca ttctcggcag
    34621 tattgcgatg cttagccagg cggcgatggc cccagcgtta ttcatcctcg gtgccgctgg
    34681 ctttacgcta tatccggtgg cgatggcatg ggcttgcgag aaagttgaac atcatcaact
    34741 ggtggcgatg aaccaggcct tactgttgag ctatactgtg ggaagtctgc ttggcccgtc
    34801 atttaccgct atgctaatgc agaatttctc cgataattta ttgtttatca tgatcgccag
    34861 cgtatcgttt atctatttgc tgatgctgct gcgcaacgcc ggtcatacgc cgaaacccgt
    34921 tgctcacgtg taaatgaatt caagcagagt gtgaacttac tgtttcacac tctgcttttt
    34981 tgtttcttct atctgacttg ctttattcca aattttattc gtttaaaaat aaaatgtgta
    35041 gcaggttata attttgcatt tcgctatttc cgcacttctt atttgccgcg cataatccct
    35101 cgttttacag atgcccctat aattttggcg aaggatttgt ctatggctgg gaatgttcag
    35161 gaaaaacagc tgcgatggta caacattgcg ctgatgtctt ttatcactgc ctggggtttt
    35221 ggcaacgttg ttaataacta cgccaaccag gggctggtgg ttgttttttc atgggtgttt
    35281 atctttgcgc tctatttcac accttatgcg ctaattgttg gtcagttagg ctcgaccttc
    35341 aaagatggga agggcggggt cagtacctgg attaaacaca cgatgggacc cggactggct
    35401 tatctcgccg cgtggaccta ctgggtggtg catattccct atctggcaca aaaaccccag
    35461 gcaattctga ttgcgctcgg ttgggcgatg aaaggcgacg gttcgctaat caaagaatat
    35521 tcagtcgtag cgttacaggg gttaacgctg gtgctgttta tcttctttat gtgggttgct
    35581 tcacgcggta tgaaatcgct gaaaatcgtc ggttctgtgg cagggattgc tatgtttgtt
    35641 atgtcgctcc tgtatgtggc gatggcggta accgcgcctg caattactga agtgcatatt
    35701 gcgaccacaa acattacctg ggaaacgttc attcctcata tcgactttac ctacattacc
    35761 actatttcaa tgctggtttt cgcggttggc ggagcagaga agatttctcc ttacgttaat
    35821 caaacgcgca acccaggaaa agaatttcca aaagggatgt tatgcctggc ggtgatggtt
    35881 gcggtttgtg ccattctggg ctcgctggcg atggggatga tgtttgattc gcgtaatatc
    35941 ccggatgact taatgaccaa cggtcagtat tacgcctttc agaagctggg cgagtattac
    36001 aacatgggta atactttaat ggtgatttac gccattgcga ataccctggg acaagtagcg
    36061 gcgctggtat tctcgattga tgccccgctt aaagtgctat taggtgatgc tgacagcaaa
    36121 tatattccag ccagtttatg tcgtaccaac gcttctggta cgcccgttaa tggctatttt
    36181 ctgaccctgg tactggtggc gattctgatt atgctgccga ctctcggcat tggtgatatg
    36241 aacaatctct ataaatggct gttgaacctt aattcggtag tgatgccgct gcgttattta
    36301 tgggtatttg ttgcatttat tgcagtcgtt cgcctggcgc agaaatataa accagaatat
    36361 gtctttattc gtaataagcc tctggcaatg accgtcggga tttggtgttt tgcctttacc
    36421 gcctttgcct gtttgacggg gatcttcccg aaaatggaag ccttcactgc agagtggacc
    36481 ttccagttgg cgctgaatgt tgcaacgccg tttgtgctgg taggattagg actgatattc
    36541 ccgctgctgg cgcgtaaagc gaatagtaaa taattattgt ggtcggtgac gctgactctg
    36601 gcagatctga cccgttttta cataatcgag aaatgcgcgc aagccgcagg acatatactg
    36661 gcggtttggg taatagatct ggaagcctgg acgctctgtg atccagtctt ccagtacaca
    36721 ttctaaacga ccggtatcaa ggtactcctt gatctgttca tagagcagat acccgatacc
    36781 agcacccatc aaaacggcat ccagttcagc atcaacatca tccagaatga tattccctgc
    36841 gacagcaatt tcctgctcct ttgcaaattg ccagtggaaa ggtttgccgc tgggataacg
    36901 aaatacaaca cattgatgat taagcagatc atgaggatgg cggggtttgc catatcttgc
    36961 aaagtactct ggcgttgctg ctacacataa tttcactggt gggccgatcg ccacgctgat
    37021 catatctttt tcgacgatgc cactaagtcg aacgccagcg tcaaagccct gttggacgat
    37081 atcgacaaga gagtcgtcag ttgtgagctc gactttaata tcaggatatt cacgtgtgaa
    37141 accaaccagt aaaggcatta aaaatatacg tgcagctacc cgggcggcat tgattttgag
    37201 cgttcctgtt ggtgtcagac gaaaatcatt catctcatct aacatgatct gaatttcgtc
    37261 gaaagctggg cgcaatcgtt catagagatt agaacccgct tccgttaacg atacgcttcg
    37321 ggttgtccga ttgaataagc gaattttaag acgttgttcc agtgttttaa tactatggct
    37381 aatggcggac gaggataagc ctaactcatc gcccgctgca cgaaagcttt gattacgggc
    37441 cacagcaaag aaagtggcaa agtcagacat attcatccgc attagtgaaa atccttcatt
    37501 aactcatcct gaatcatcac gtttacatga ttatgccgcc tgcgtaaagt gcgctccaga
    37561 acttaacgtg gaggtaaaat tatgcagtct gaacgtattt atttggtatg ggcccatcct
    37621 cgtcatgatt cattgaccgc acatattgct gatgcgatcc atcagcgggc aatggagcgg
    37681 aaaatacagg tgacggaact cgatttatat cggcgtaatt tcaacccggt gatgacgccg
    37741 gaagatgaac cagactggaa gaatatggat aaacgttatt ctccagaggt tcatcagctt
    37801 tattcagagc tgcttgaaca tgacacgtta gtggtggttt ttcctgtctg gtggtacagc
    37861 ttcccggcaa tgctaaaagg atatattgac agagtatgga ataatgggct ggcttatgga
    37921 gatgggcaga aattaccatt caataaagtt cgttgggtgg cgctggttgg aggagacaaa
    37981 gaatcatttg tccagatggg ctgggaaaaa aatataagcg attatttaaa aaatatgtgc
    38041 agttatcttg gtattgaaga tgccgatgtc actttcttgt gtaatacagt ggtattcgat
    38101 ggggaagaac ttcacgcgag ctattatcag tcgttattat ctcaggtacg ggatatggta
    38161 gatgcactat aagatgtgtt aaaaacgctg tagcagaatg aagtgcggaa taaaaaagcg
    38221 gcaactcaat aaagttgccg ctttacgggg aaattagaac attaccttat gaccgtactg
    38281 ctcaagaatg cctttcacgc gttccatggt ctctttcttc ggtggtttaa caccgtcgag
    38341 tttgtactct tcacccattg ccacccattt gtgtttgccc agctcgtggt aggggagaag
    38401 ctcgattttc tcaacgttgc ccatatcacg ggtaaattca ccgaggcgat gcgctgaatc
    38461 gtcatcgtca gaccagcctg ggacaacaac gtagcggatc cacaccttca catttttgtt
    38521 cgccagatat ttggcgaact ccagcgtgcg gtgattggaa actccaacca gattttggtg
    38581 gatctcgtcg ttcatctgtt tgagatcgag cattaccagg tcggttactt ccagcagttc
    38641 atcaatcacc ggatcgtaac gacgaacaaa accgttggtg tccagacagg tatgaatgcc
    38701 ttcttttttg caggcgcgga accagtcacg aacaaactca gcttgtagga ttgcctcacc
    38761 gccggatgcg gtaacgccgc cgccggaagc gttcataaag tggcgatagg tcaccacctc
    38821 tttcattaaa tcttcaacgg taacttcttt accgccatgc gtatcccagg tgtcgcggtt
    38881 atgacaatac aggcagcgca tcaggcagcc ctggaaaaag gtgataaagc gaatacccgg
    38941 gccgtctacg gttccacagg attcaaagga gtgaatgcga ccaataactg acattgcggt
    39001 gtttctccag atgtggccca tctgaggccg tgttggtgcg cagctcgaag gctacgtcga
    39061 gtctgttttg gcagtcacct tagagtatag atagctgaca aaaaaggctc tcgtgctaaa
    39121 aaaggcccca ctttcgtgga gcctttattg tacgcttttt actgtacgat ttcagtcaaa
    39181 tctaattaca tagattgagt gaaggtacga gtaataacgt cctgctgctg ttctttagtc
    39241 agcgagttga aacgtactgc gtagccagat acacggatgg tcagctgcgg atatttttcc
    39301 gggttttcca tcgcgtcgag cagcatttca cggttcatca cgttaacgtt caggtgctga
    39361 ccaccttcga tggatgcttc gtggtggaag taaccatcca tcagaccagc caggttggtc
    39421 ttacgaactt cgtcgtcttt acccagtgcg ttcggaacga tagagaaggt gtaggagata
    39481 ccatctttag cgtaagcaaa cggcagttta gcaacggaag tcagagaggc tactgcacct
    39541 ttctggtcac gaccgtgcat cgggttagca cccggtccga acggcgcgcc agcacgacga
    39601 ccgtctgggg tgttacccgt tttcttacca tacacaacgt tagaagtgat ggtcagaaca
    39661 gactgagtcg ggatagcgtc acggtaggtg tgcagtttct gaattttctt catgaaacgt
    39721 tctaccaggt caacagccag gtcatctaca cgcggatcgt tgttaccaaa ctgcgggtat
    39781 tcgccttcga tttcgaagtc gatagccaga ccgtcttcgt cacgaatcgg tttaactttc
    39841 gcatatttga ttgcagacag ggagtcagca gcaacggaca gaccagcgat accacacgcc
    39901 atggtgcgga taacgtcacg gtcgtgcagc gccatcagag aggcttcgta gctgtacttg
    39961 tcgtgcatgt agtggatgat gttcagtgca gtgatgtact gtttagccag ccagtccatg
    40021 aagtgatcca tgcgctccat cacttcatca tagttcagga catcgccttt gatcggttca
    40081 gacttcggac caacctgcat tttcagtttt tcgtcaacgc cgccgttgat tgcgtacagc
    40141 atggttttcg ccaggtttgc acgcgcaccg aagaactgca tttgtttacc aacgatcatc
    40201 gggcttacgc agcaagcaat agcgtagtca tcgttgttga agtccggacg catcaggtca
    40261 tcgttctcat actgcagaga agaggtgtcg atggacactt tagcggcgaa tttcttgaag
    40321 ttcagcggca gtttttcaga ccacagaatg gtcatgttcg gttccggaga cggacccatg
    40381 gtgtacaggg tgttcaggaa acggaagctg tttttggtaa ccagggtacg accgtcgagg
    40441 cccataccac cgatagattc ggttgcccag atcgggtcgc cagagaacag ttcatcgtat
    40501 tccggagtac gcaggaagcg aaccatacgc agtttcatga ccaggtggtc aaccatttcc
    40561 tgcgcttctt gttcggtgat cttgccagct ttcaggtcac gttcgatgta cacatccagg
    40621 aaggtggagg tacgaccgaa ggacattgca gcaccgttct gagacttaac agcagccagg
    40681 tagccgaagt aagtccactg gatagcttcc tgagcgttgg tagccggacc agagatgtcg
    40741 tagccgtatt tcgcagccat ttctttcatc tgacccagag cgcggtgctg ttcagcgatt
    40801 tcttcgcgca gacggatagt ctgttccaag tttacgccgt tttccagatc agcctgcaga
    40861 gaagtgaact gtgccagttt gtctttcatc aggtagtcga taccgtacag cgcaacgcga
    40921 cggtagtcac cgatgatacg gccacggcca tatgcatctg gcagaccggt cagaacacca
    40981 gatttacggc aacgcaggat gtccggagtg taaacgtcga acacgccctg gttgtgagtt
    41041 ttacggtatt cagtgaagat ttttttgatc atcggatcca gttcgcggtt gtacgctttg
    41101 caggaacctt cgatcatttt gataccaccg aacgggataa gagcacgttt cagcggagct
    41161 tcagtctgca gaccaacgat tttctcaagc tgcttgttga tgtagccagc gtcgtgagag
    41221 gtgatggtgg aagcaacagc ggtgtcaaag tcaactggcg cgtgagtgcg gttttccagt
    41281 ttaacgcctt ccattacttt gtcccacagg gtggtggtcg cttcagtagc gccagccagg
    41341 aaggactcgt caccctcgta cggagtgtag tttttctgaa tgaagtcacg gacgtttact
    41401 tcattctgcc agtcaccttt ggtaaaacct tcccaggctg tggctaactt ttcattaagc
    41461 tcggacatgt aacacctacc ttcttaagtg gattttttat ttactgcgta cttcgacaac
    41521 cattaatggt ggtcgttttc acgcaggtaa atgacccagt atgtcaaccc aaccaacaaa
    41581 ccaccaccga taatgttgcc gatcgtaacc ggaatcaggt tatcagtgat gaaattcatc
    41641 acggtcaggt gagaaaaatt ttccggtgca gaaccgactg cggtccaaaa ttccggggat
    41701 gcgaagtcgc ggattacaat acccatcggg atcataaaca tgtttgcgat actgtgctca
    41761 aaaccgctgg caacaaacat cgcgaccggc agcaccataa tgaacgcttt gtccatcagg
    41821 ctgcggccag aataactcat ccatactgcc agacatacca tcaggtttgc caggatacca
    41881 agacagacgg cctcaataaa agtatggtgc actttgtggt cggcggtttg taggacgttt
    41941 agtccccatt gaccatttgc ggtcatatac tcgccggaaa gccacattaa aagtacaaac
    42001 agcagtgcgc cgaccaggtt gccaaaatag acatttagcc agtttttcgc caactgaccc
    42061 caggtgatgc gcccactcgc cttagcaaca acaatcaaca cggtggaagt aaagagatcg
    42121 gctccgcaga caacacaaag aatcagcccc agagagaagc aaatgccgcc aaccagtttt
    42181 gccatgccga agggcattgt gcctgtgcca gtggttgctg tgatatagaa gacgaatgcg
    42241 attgagatga aaacaccggc ggtaatcgcc agatagaaag tcttaagcgg atgtttcgtt
    42301 gctttataga cacccgcctc ttcggccact ttggccattg cagcaggaag taaaagatca
    42361 aaagggttgt cagctttcac actaactctc tctttattaa gtcggcgacg agatactaac
    42421 aaagcattat agatgagaaa ttgatataga tcatatctcg cctggcttat aggcccgtaa
    42481 ctcgcatggt ttttatgcag atacggagta aatatttgat tatccaaata aaaataaatt
    42541 ttaaaaatta acaaatgagt tgaatttttt ccgcatcctc cgctaaaaca gttaattaaa
    42601 agggagcatc aggcgaataa agtaacaata tcgatcgtat ttattaaata caaattaccg
    42661 atatttaacc ttataattac aattatttta ttaatgcaaa tatatgtaaa gcggggcatt
    42721 aaaaaaacgc cccgtaatat aactcagact aatcattaag cctacattgc gtaggctatt
    42781 tgattttatt ttgcccagaa tgctgctttg gcgcgctgca gcttttcgta ggccttcaac
    42841 aacgactgat gtgcagcaaa cgcgtgcaga tcgctatcta ccggttgcag gccgtaaaac
    42901 gccgcttcgc cgctcattgc cgcactggcg gcttctacgg catctgcgcc gtacatgcga
    42961 acaaacgcat tcagatattg cagcggctgg cgatcttctt cctgtgccag taataacaac
    43021 gtttgcaggc agcgataata gttggcgcgt tccggactaa atactgatga gttaaactcc
    43081 atcgtccatt cggtccagac cagagcctgt tccagatcgc caccagccag cgccagcatg
    43141 gcttttaatt caccgatacg cagggtgtac caaccgttat ccgacccggt cgccagaccc
    43201 aacagctcac gcacgcgggt aaagtcatca aaaccttctt catccagttg ctcgatgagg
    43261 ttcaggtaat cttctttttc ccactcgctg cctggtagcg aaagaatcgt ttcacgtaaa
    43321 tggctgccca tactgttatt cgcgagccac agatcttcag ccggataaat atcggacatg
    43381 ccaggcacga taatacggca agcataaacg cccagatgct cgtaatcggc aatataaact
    43441 tctttatctt ctttgttgaa gatagccatc agcgtagcaa actcttcttc cgtggtgccg
    43501 gagaaattcc agtccacaaa cggataatcg gcatcctgct tgaacaggtc ccaggagatt
    43561 aaaccgctgg aatcgataaa gtgcgtttcg aggttggtat gttcagcgac ttcttcatca
    43621 tcgaaggttg gcggagtaaa cacatccaaa tctttcaggc cacgaccttg cagcagctcg
    43681 gtcacggtac gttccagtgc tacgccaaaa tcaggatgcg caccgaaaga ggcaaagcag
    43741 gtaccgttag caggattgaa cagtaccacg caaatcaccg gatactggcc gccaagcgaa
    43801 ccgtcataag cgaagattgg gaaaccctcc gcttccagcg tttcgatggc ttcaactact
    43861 gctgggtaac gcgccagcac gtctgccggg atctctggca ggctgatgct ttcagcaata
    43921 atgcggtttt tcacgtagcg ttcgaaaact tcagacaatc cctgaacgcg tgcttcgttg
    43981 cgggtattac ctgcggacat accgttggag acgtacaggt taccaatgat attcatcgga
    44041 atataaacgg tctgattgtc ggactgacgc gtaaacggca ggccgcaaat accacgatct
    44101 tcgttaccgg attgtaggtc aatcagcatg ctgccggtca gttcattctc cggatcataa
    44161 aacgcgcgca gacggtcatc gagtagtcct tctggcacat cgtcattttc ggtcagtggg
    44221 aaccattttt cgttgggata atgcacgaac ggaccgttgg cgatggtttc gcccagccag
    44281 aaatcagcaa aaaagtagtt ggttgagaga cgctcgaaat attcaccaag tgcagaagcc
    44341 agcgccgctt ttttggttgc gcctttaccg ttggtaaaac acagtgcgca ctctttgtcg
    44401 cgaatatgta cagaccagac gttaggcaca ggattcagcc aggaggcctc ttcaatctga
    44461 aagccgaggt ctgaaagttt ttgctggaag cgagcgatgg aatcttccag agcggcatct
    44521 ttgccgggga taaatgtttg cgtcatgaaa atcactttag tcgtacggaa agcgcgcaat
    44581 aatacgggtt ttatctcaaa ggcgctatca ccgccgccat gccgatgaac tgttgactat
    44641 gcttttagcg gataaaccac gataagagca taaaaatgaa ggcgttcgat ctccaccgta
    44701 tggcatttga taaagtgcct tttgatttcc ttggcgaagt tgcactacgt agtctttata
    44761 cctttgtact ggtttttttg ttcctcaaaa tgaccggaag acgcggtgtg cgacagatgt
    44821 cgctgtttga agtgttaatc attctcacgc tgggttcagc ggcgggagat gtggcgtttt
    44881 atgatgatgt gccgatggtg ccggtactta tcgtctttat cactctggcg ttgttatacc
    44941 gcctggtaat gtggttgatg gcgcacagtg aaaaactgga agatctactg gaaggtaagc
    45001 cggttgttat tattgaagat ggcgagctgg cctggtcgaa actcaataac tccaacatga
    45061 cggaatttga gttctttatg gagctacgat tgcgtggcgt ggagcagctg gggcaggtac
    45121 gtctggcgat tctcgaaacc aacgggcaaa tcagtgtcta tttctttgaa gatgacaagg
    45181 tgaaaccggg tttacttatt ttacccagtg attgtacgca gcgttacaaa gtggtgccgg
    45241 agtcggcgga ctatgcctgt attcgttgta gtgaaatcat tcatatgaaa gcgggggaaa
    45301 aacaattatg tccgcgctgt gcaaatccag aatggacgaa ggcaagtcgg gcaaaacggg
    45361 tgacctgaca gtaaaaacat cggctttttg ctaataatcc gagagattct tttgtgtgat
    45421 gcaagccaca tttttgccct caacggtttt actcattgcg atgtgtgtca ctgaatgata
    45481 aaaccgatag ccacaggaat aatgtattac ctgtggtcgc aatcgattga ccgcgggtta
    45541 atagcaacgc aacgtggtga ggggcaatgg ctcaaatctt caattttagt tctggtccgg
    45601 caatgctacc ggcagaggtg cttaaacagg ctcaacagga actgcgcgac tggaacggtc
    45661 ttggtacgtc ggtgatggaa gtgagtcacc gtggcaaaga gttcattcag gttgcagagg
    45721 aagccgagaa ggattttcgc gatcttctta atgtcccctc caactacaag gtattattct
    45781 gccatggcgg tggtcgcggt cagtttgctg cggtaccgct gaatattctc ggtgataaaa
    45841 ccaccgcaga ttatgttgat gccggttact gggcggcaag tgccattaaa gaagcgaaaa
    45901 aatactgcac gcctaatgtc tttgacgcca aagtgactgt tgatggtctg cgcgcggtta
    45961 agccaatgcg tgaatggcaa ctctctgata atgctgctta tatgcattac tgcccgaatg
    46021 aaaccatcga cggtatcgcc atcgacgaaa cgccagactt cggcaaagat gtggtggtcg
    46081 ccgccgactt ctcttcaacc attctttccc gtccgattga cgtcagccgt tatggcgtga
    46141 tttacgctgg cgcgcagaaa aatatcggcc cggctggcct gacaatcgtc atcgttcgtg
    46201 aagatttgct gggcaaagcg aatatcgcgt gtccgtcgat tctggattat tccatcctca
    46261 acgataacgg ctccatgttt aacacgccgc cgacatttgc ctggtatcta tctggtctgg
    46321 tctttaaatg gctgaaagcg aacggcggtg tagctgaaat ggataaaatc aatcagcaaa
    46381 aagcagaact gctatatggg gtgattgata acagcgattt ctaccgcaat gacgtggcga
    46441 aagctaaccg ttcgcggatg aacgtgccgt tccagttggc ggacagtgcg cttgacaaat
    46501 tgttccttga agagtctttt gctgctggcc ttcatgcact gaaaggtcac cgtgtggtcg
    46561 gcggaatgcg cgcttctatt tataacgcca tgccgctgga aggcgttaaa gcgctgacag
    46621 acttcatggt tgagttcgaa cgccgtcacg gttaatgccg aaattttgct taatccccac
    46681 agccagcctg tggggttttt atttctgttg tagagagttg agttcatgga atccctgacg
    46741 ttacaaccca tcgctcgtgt cgatggcact attaatctgc ccggttccaa gagcgtttct
    46801 aaccgcgctt tattgctggc ggcattagca cacggcaaaa cagtattaac caatctgctg
    46861 gatagcgatg acgtgcgcca tatgctgaat gcattaacag cgttaggggt aagctatacg
    46921 ctttcagccg atcgtacgcg ttgcgaaatt atcggtaacg gcggtccatt acacgcagaa
    46981 ggtgccctgg agttgttcct cggtaacgcc ggaacggcaa tgcgtccgct ggcggcagct
    47041 ctttgtctgg gtagcaatga tattgtgctg accggtgagc cgcgtatgaa agaacgcccg
    47101 attggtcatc tggtggatgc gctgcgcctg ggcggggcga agatcactta cctggaacaa
    47161 gaaaattatc cgccgttgcg tttacagggc ggctttactg gcggcaacgt tgacgttgat
    47221 ggctccgttt ccagccaatt cctcaccgca ctgttaatga ctgcgcctct tgcgccggaa
    47281 gatacggtga ttcgtattaa aggcgatctg gtttctaaac cttatatcga catcacactc
    47341 aatctgatga agacgtttgg tgttgaaatt gaaaatcagc actatcaaca atttgtcgta
    47401 aaaggcgggc agtcttatca gtctccgggt acttatttgg tcgaaggcga tgcatcttcg
    47461 gcttcttact ttctggcagc agcagcaatc aaaggcggca ctgtaaaagt gaccggtatt
    47521 ggacgtaaca gtatgcaggg tgatattcgc tttgctgatg tgctggaaaa aatgggcgcg
    47581 accatttgct ggggcgatga ttatatttcc tgcacgcgtg gtgaactgaa cgctattgat
    47641 atggatatga accatattcc cgatgcggcg atgaccattg ccacggcggc gttatttgca
    47701 aaaggcacca ccacgctgcg caatatctat aactggcgtg ttaaagagac cgatcgcctg
    47761 tttgcgatgg caacagaact gcgtaaagtc ggtgcggaag tagaagaggg gcacgattac
    47821 attcgtatca ctccaccgga aaaactgaac tttgccgaga tcgcgacata caatgatcac
    47881 cggatggcga tgtgtttctc gctggtggcg ttgtcagata caccagtgac gattcttgat
    47941 cccaaatgta cggccaaaac atttccggat tatttcgagc agttggcgcg gattagccag
    48001 gcagcctgaa tgaacaacgg gcaataaata gccaaatctt tctttatcaa aacgtcggca
    48061 cattg
//
LOCUS       DAKISA010000021        47250 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.021, whole genome shotgun sequence.
ACCESSION   DAKISA010000021 DAKISA010000000
VERSION     DAKISA010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47250
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.021"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..1504)
                     /locus_tag="OVW45_001223"
     CDS             complement(<1..1504)
                     /locus_tag="OVW45_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HCV5525727.1"
                     /translation="MVLLSAGVWALQYAGSGPEKTLSPLVVHNNLQIDLNEPDLFLDS
                     DSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIEGSIRRIVYEHDLTLKDKLFS
                     SLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPLLFAATSDSQLSKTEISSIKI
                     NSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFKDDQQDTEATAIAGDLLSGKK
                     RWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMPSFAGVRFEMGNDGWHSFVAL
                     NDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAEEMLSHISQENDKLNGALDGA
                     AGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNIGAHESKAPEGVLPISQTQQG
                     EAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNNALQ
                     TLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLTSLPKNLEPVFYNA"
     gene            complement(1712..4339)
                     /gene="gyrA"
                     /locus_tag="OVW45_001224"
     CDS             complement(1712..4339)
                     /gene="gyrA"
                     /locus_tag="OVW45_001224"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCV5525728.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHILGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            4486..5208
                     /locus_tag="OVW45_001225"
     CDS             4486..5208
                     /locus_tag="OVW45_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCV5525729.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(5336..9088)
                     /gene="yfaL"
                     /locus_tag="OVW45_001226"
     CDS             complement(5336..9088)
                     /gene="yfaL"
                     /locus_tag="OVW45_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HCV5525730.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVTGEYYNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLSVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPPGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLSGAQSMALTGDIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYNDGLEVSGSSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANN
                     NSYLGTTQIASGTLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADIFPYASSLWVGDGAAFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGDLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPSPPNPNPTPDPDPTPDPDPTPDPAPTPAYQPVLNAKVGGYLNNLRAANQAF
                     MMERRDHAGGDGQTLNLRVIGGRYHYTAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEW
                     MLGAVGSYSDNQGDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDNWLQYA
                     WFSNDVSEHEDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAA
                     NHARVSQSQGDDIQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAV
                     KRRGEIKVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            9784..12069
                     /gene="nrdA"
                     /locus_tag="OVW45_001227"
     CDS             9784..12069
                     /gene="nrdA"
                     /locus_tag="OVW45_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCV5525731.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            12303..13433
                     /gene="nrdB"
                     /locus_tag="OVW45_001228"
     CDS             12303..13433
                     /gene="nrdB"
                     /locus_tag="OVW45_001228"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCV5525732.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            13433..13687
                     /gene="yfaE"
                     /locus_tag="OVW45_001229"
     CDS             13433..13687
                     /gene="yfaE"
                     /locus_tag="OVW45_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCV5525733.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(13764..14390)
                     /gene="inaA"
                     /locus_tag="OVW45_001230"
     CDS             complement(13764..14390)
                     /gene="inaA"
                     /locus_tag="OVW45_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCV5525734.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRNKAINHDFRQLENIWSQSPKRTGSR"
     gene            14569..14811
                     /locus_tag="OVW45_001231"
                     /pseudo
     CDS             14569..14811
                     /locus_tag="OVW45_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(14853..15929)
                     /gene="glpQ"
                     /locus_tag="OVW45_001232"
     CDS             complement(14853..15929)
                     /gene="glpQ"
                     /locus_tag="OVW45_001232"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCV5525735.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYSYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(15934..17292)
                     /gene="glpT"
                     /locus_tag="OVW45_001233"
     CDS             complement(15934..17292)
                     /gene="glpT"
                     /locus_tag="OVW45_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCV5525736.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            17565..19193
                     /gene="glpA"
                     /locus_tag="OVW45_001234"
     CDS             17565..19193
                     /gene="glpA"
                     /locus_tag="OVW45_001234"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCV5525737.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            19183..20442
                     /gene="glpB"
                     /locus_tag="OVW45_001235"
     CDS             19183..20442
                     /gene="glpB"
                     /locus_tag="OVW45_001235"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCV5525738.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            20439..21629
                     /gene="glpC"
                     /locus_tag="OVW45_001236"
     CDS             20439..21629
                     /gene="glpC"
                     /locus_tag="OVW45_001236"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCV5525739.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGIGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            21822..22724
                     /locus_tag="OVW45_001237"
     CDS             21822..22724
                     /locus_tag="OVW45_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCV5525740.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVKGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEISERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(22765..23568)
                     /gene="yfaU"
                     /locus_tag="OVW45_001238"
     CDS             complement(22765..23568)
                     /gene="yfaU"
                     /locus_tag="OVW45_001238"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HCV5525741.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVASATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(23586..24875)
                     /locus_tag="OVW45_001239"
     CDS             complement(23586..24875)
                     /locus_tag="OVW45_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCV5525742.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            complement(24932..26137)
                     /gene="rhmD"
                     /locus_tag="OVW45_001240"
     CDS             complement(24932..26137)
                     /gene="rhmD"
                     /locus_tag="OVW45_001240"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HCV5525743.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAVMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            complement(26152..26934)
                     /locus_tag="OVW45_001241"
     CDS             complement(26152..26934)
                     /locus_tag="OVW45_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCV5525744.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            complement(27154..28356)
                     /locus_tag="OVW45_001242"
     CDS             complement(27154..28356)
                     /locus_tag="OVW45_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCV5525745.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVRLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQSPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(28456..28998)
                     /locus_tag="OVW45_001243"
     CDS             complement(28456..28998)
                     /locus_tag="OVW45_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525746.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            29277..29702
                     /gene="nudI"
                     /locus_tag="OVW45_001244"
     CDS             29277..29702
                     /gene="nudI"
                     /locus_tag="OVW45_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCV5525747.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(29741..30343)
                     /locus_tag="OVW45_001245"
     CDS             complement(29741..30343)
                     /locus_tag="OVW45_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCV5525748.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            30651..31790
                     /gene="arnB"
                     /locus_tag="OVW45_001246"
     CDS             30651..31790
                     /gene="arnB"
                     /locus_tag="OVW45_001246"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCV5525749.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            31794..32762
                     /gene="arnC"
                     /locus_tag="OVW45_001247"
     CDS             31794..32762
                     /gene="arnC"
                     /locus_tag="OVW45_001247"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCV5525750.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            32762..34744
                     /gene="arnA"
                     /locus_tag="OVW45_001248"
     CDS             32762..34744
                     /gene="arnA"
                     /locus_tag="OVW45_001248"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HCV5525751.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPAEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGHRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            34741..35631
                     /locus_tag="OVW45_001249"
     CDS             34741..35631
                     /locus_tag="OVW45_001249"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCV5525752.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            35631..37283
                     /gene="arnT"
                     /locus_tag="OVW45_001250"
     CDS             35631..37283
                     /gene="arnT"
                     /locus_tag="OVW45_001250"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCV5525753.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGFTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            37280..37615
                     /gene="arnE"
                     /locus_tag="OVW45_001251"
     CDS             37280..37615
                     /gene="arnE"
                     /locus_tag="OVW45_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCV5525754.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            37615..38001
                     /gene="arnF"
                     /locus_tag="OVW45_001252"
     CDS             37615..38001
                     /gene="arnF"
                     /locus_tag="OVW45_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCV5525755.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(37995..38261)
                     /gene="pmrD"
                     /locus_tag="OVW45_001253"
     CDS             complement(37995..38261)
                     /gene="pmrD"
                     /locus_tag="OVW45_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCV5525756.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(38371..39726)
                     /gene="menE"
                     /locus_tag="OVW45_001254"
     CDS             complement(38371..39726)
                     /gene="menE"
                     /locus_tag="OVW45_001254"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCV5525757.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     D"
     gene            complement(39723..40685)
                     /gene="menC"
                     /locus_tag="OVW45_001255"
     CDS             complement(39723..40685)
                     /gene="menC"
                     /locus_tag="OVW45_001255"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCV5525758.1"
                     /translation="MRSAQVYRWQIPMDAGMVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQIVLLAWVNNWLAGDGELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(40685..41542)
                     /gene="menB"
                     /locus_tag="OVW45_001256"
     CDS             complement(40685..41542)
                     /gene="menB"
                     /locus_tag="OVW45_001256"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCV5525759.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(41557..42315)
                     /gene="menH"
                     /locus_tag="OVW45_001257"
     CDS             complement(41557..42315)
                     /gene="menH"
                     /locus_tag="OVW45_001257"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCV5525760.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VYLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILNFWLVGYSLGGRVAMMAACQGMPG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(42312..43982)
                     /gene="menD"
                     /locus_tag="OVW45_001258"
     CDS             complement(42312..43982)
                     /gene="menD"
                     /locus_tag="OVW45_001258"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCV5525761.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     SGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLILIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(44071..45366)
                     /gene="menF"
                     /locus_tag="OVW45_001259"
     CDS             complement(44071..45366)
                     /gene="menF"
                     /locus_tag="OVW45_001259"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCV5525762.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADNVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(45445..45750)
                     /gene="elaB"
                     /locus_tag="OVW45_001260"
     CDS             complement(45445..45750)
                     /gene="elaB"
                     /locus_tag="OVW45_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCV5525763.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(45805..46266)
                     /locus_tag="OVW45_001261"
     CDS             complement(45805..46266)
                     /locus_tag="OVW45_001261"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCV5525764.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            46331..47248
                     /gene="rbn"
                     /locus_tag="OVW45_001262"
     CDS             46331..47248
                     /gene="rbn"
                     /locus_tag="OVW45_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCV5525765.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT        11433 a        12812 c        11698 g        11307 t
ORIGIN      
        1 cggcgttata aaaaaccggt tcgagattct tcggcagact ggtcaacgtt tcgttacgca
       61 gcagtttcgc cacgccttgt ggattgatat agagcggaac gatgccatca gtgggtatta
      121 catccaccat tgccgggcgg tttttattca gtgtttgcag ggcgttatta accagcgtgt
      181 catcgaggga gaaaagcagc gttttgtttt gcatcgccag cgacacgcgg aaaaaataat
      241 ccgacattaa ttgatcgggt tgcgccgcct gcgctttcgg atactgaccg tatcgggaac
      301 tcacttcgcg acgccagatt tgcgcttcgc cctgctgagt ctggcttatc ggcaaaacac
      361 cttctggcgc tttgctttcg tgcgcaccaa tattttgcgt aaacagtttc cctggcaatt
      421 gcgcctgttc ggcagtgcca tcaaactgac cgacaaacag cggggtttgc aattttgagt
      481 cttcatacca gcacagcccc gcggcaccgt ctaacgcccc attcagcttg tcgttttcct
      541 ggctgatgtg cgaaagcatc tcttcggcaa taccgtgtga atacggcacc accacacaga
      601 agctggcccc ggcaggcata ctgttccaga ccggcgtaaa atcgaaactg gtatctacgc
      661 tggcggattc atcatttaac gccacaaaac tgtgccagcc gtcgttaccc atttcgaagc
      721 gtacgccagc aaaagaaggc attaaccgct ggtagccaaa acccagcaac ctggcgctga
      781 ccacgatgcg ctggcgtact ggcgtttttt cagcagcacg ctcttccagg ccaaagcttg
      841 cttgccagcg ttttttgccg ctcaacaaat cacctgcgat cgccgtggct tcggtatcct
      901 gctgatcatc tttaaacaac atatccgtgc tggaaaacac cagcatcttg tcctgataag
      961 tcgcgaacat cagggcgtta ttgccgttat agcgcaactg ataaacagga atagtttcac
     1021 tatttatctt gatgctactg atttccgttt tgcttaactg gctgtcgctg gtagcggcaa
     1081 acaacaatgg ctccagcagt ttgcttaaac cactgcgctg gatcagcacc atgtaatgtg
     1141 aaagatggcc ttgtttatcg tgccatagcg cagcctgcgc gggctgatct aagagtgacg
     1201 aaaagagttt atctttcagc gtgagatcgt gttcatagac aatgcgacga atgctgcctt
     1261 caatgcccag acgatcggca tgattctgat aatagaaaac gaaatcttca ctcagaacat
     1321 cgtggagaaa cggaatggtg aggagatctt tgggaagctg gctcagagag tcgctgtcga
     1381 gaaagaggtc cggctcattg agatcgattt gcagattgtt gtgcaccacc agcggcgaca
     1441 acgttttttc tggcccactg ccagcatatt gcaacgccca gacaccagcg gaaagcagta
     1501 ctattgcgcc aaaacctaca agaccataga accgccagcc tttcgctttt tttcaccact
     1561 cattgccaca ttccttgtgt atagccagcc attttttacg ggcacagcca aactttaccg
     1621 tgccctaata cgacaaaagc ccagactttg cagcctggac ttttcaattc aaacaaggga
     1681 gatagctccc ttttggcatg aagaagtaaa attattcttc ttctggctcg tcgtcaacgt
     1741 ccacttccgg agcgatttca tcgtcccctt ccgcggcact gccgtcgatg gtatcgagat
     1801 cttcctcgtc aaccggttca gcaacacgtt gcagacccac tacgttttca tcttccgcag
     1861 tacggatgag gatcacgccc tgggtgttac ggcccacgat gctgatttct gaaacgcgag
     1921 tacgtaccag cgtaccggca tcggtgatca tcatgatctg gtcgcagtca tctacctgta
     1981 ccgcgccaac cactaaaccg ttacgttcgg taaccttgat ggagataacc cctttcgtcg
     2041 cacgcgactt ggttgggtat tccgccactg cggtacgttt accgtaaccg ttttgcgttg
     2101 cggtgaggat tgcgccatcg ccacgaggca cgatcagaga gacgacttta tcgccttcac
     2161 ctaagcgaat accgcgaaca ccggtggtgt tgcagcccat cgcacggaca gaagactctt
     2221 taaagcgcac cactttacct tcggcggaga acagcattac ttcgtcttcg ccgctggtca
     2281 ggtcaacgcc gatcagctca tcgccttcaa ccagtttgat cgccacttta ccggcggtac
     2341 gcagacggtt gaattcggtg aggacggttt tcttcacagt accgttagcg gtcgccatga
     2401 agactttcac gccttcttca aactcggtca ctggcaggat cgcggtgata cgttcgtcct
     2461 gctccagcgg cagcaggttg acgatcggac gaccgcgcgc gccacgagtg gcctccggca
     2521 actggtaaac tttcatcgaa tagacgcgac cacggctgga gaagcacaga atatggtcgt
     2581 gcgtgtttgc caccagcagt cggtcgataa agtcttcttc tttaatacgt gcggcagatt
     2641 tacctttccc gccacgacgc tgcgcttcgt attcagaaag cggctgatat ttaacatagc
     2701 cctggtgaga gagcgtcacg accacatctt cctgggtgat cagatcttcc aggttgatgt
     2761 ctgcgctgtt ggcggtgatt tcagtacgac gtttgtcacc gaactgttca cgaaccagct
     2821 ccagctcttc acggatcact tccatcagac gatcggcgct accaagaata cgcaacagtt
     2881 ccgcgatctg atccagcagc tctttgtatt cgtcgagcag tttttcgtgc tcaaggccgg
     2941 tcagtttctg caaacgcaga tccagaatcg cctgagcttg ctgttcggtc aggtagtaca
     3001 gaccatcacg cacgccgaac tctggctcca gccattccgg acgcgcagca tcgtcgccag
     3061 cacgttccag catcgcggca acgttgccca gctgccacgg attagccacc agcgcagttt
     3121 tcgcttctgc aggcgtcggc gcatgacgga tcagttcgat gatcgggtcg atgttcgcca
     3181 gcgccacggc tagtgcttca aggatatgag cacgatcgcg agctttacgc agttcgaaaa
     3241 tagtacgacg ggtcaccact tcacggcggt gacgaacaaa cgccgcgatg atatctttca
     3301 ggttcatgat cttcggctga ccatggtgca atgccaccat gttgataccg aaagaaacct
     3361 gcaactgggt ctgggagtag aggttgttga gcacaacttc accgaccgca tcgcgtttaa
     3421 cttcaatcac gatgcgcata ccgtctttgt cagactcgtc acgcagcgcg ctgatgcctt
     3481 ccacgcgttt ttcttttacc agttccgcaa tcttctcgat caggcgcgct ttgtttacct
     3541 gatacggaat ttcgtggacg ataatggttt cacgcccggt tttggcgtcg acttccactt
     3601 cagcacgggc gcggatgtac accttgccac gaccggtacg gtaagcttct tcaataccgc
     3661 gacgaccgtt aatgattgcc gccgtcggga agtccggccc caggatgtgt tccatcagcc
     3721 cttcaatgct gatgtcttca tcatcgatat acgccagaca accgttgatg acttccgtca
     3781 ggttgtgcgg cgggatgttg gttgccatac ctacggcgat accggaagaa ccgttcacca
     3841 gcaggttagg aattttggtt ggcatgacgt ccggaatttt ttccgtgccg tcatagttat
     3901 caacgaaatc gaccgtctct ttttcgagat cggccatcag ttcatgggca attttcgcca
     3961 gacggatttc cgtataacgc attgccgccg cagagtcgcc gtcgatggaa ccgaagttac
     4021 cctgaccgtc taccagcatg taacgcagcg agaatggctg cgccatacgg acgatcgtgt
     4081 cataaaccgc cgagtcacca tggggatggt atttaccgat tacgtcacca acgacacggg
     4141 cagatttttt ataggctttg ttccagtcat tgcctagtac gttcatggcg taaagtacgc
     4201 gacggtgtac cggcttcagg ccatctcgga catccggcag cgcacggcca acaatgaccg
     4261 acatcgcata atccagatag gagctcttca gctcttcctc aatgttgacc ggtgtaattt
     4321 ctctcgcaag gtcgctcatc taaccgctat ccctctactg tatcccggat tcaaaggtcg
     4381 caaattataa cacagccgcg cagtttgagg taaacctata cgctttattc acatccaatg
     4441 cctgatatac tcgtttgtct tgccaattac ggagtagaag tgccaatgaa tgccgaaaaa
     4501 tcgccggaaa accataacgt agaccacgaa gagatcgcta aatttgaagc cgtcgcctcc
     4561 cgctggtggg atcttgaagg tgagttcaaa ccgttgcacc gcattaaccc gctgcgtctg
     4621 ggctatattg ccgagcgtgc tggcggttta tttggcaaaa aggtgctcga tgtcggttgt
     4681 ggcggcggca ttctggccga gagtatggcg cgcgaaggcg cgacggtgac cggtctggat
     4741 atgggctttg agccattgca ggtggcaaag ctgcacgcgc tggaaagcgg cattcaggtg
     4801 gattacgtgc aggaaaccgt ggaagagcac gcggcaaaac atgccgggca gtatgatgtg
     4861 gtgacctgca tggagatgct ggagcacgta cccgatccgc agtcagtggt cagagcctgt
     4921 gcgcaactgg tgaaaccagg cggcgatgtc tttttctcga cgcttaaccg caacggcaag
     4981 tcatggctga tggcggtggt tggcgcggaa tatattttgc gcatggtgcc caaaggtacg
     5041 catgatgtga agaagtttat taaaccggca gagctgcttg gctgggtgga tcagaccagt
     5101 ttaaaagagc gacatataac cgggctacat tacaacccga tcactaattc ctttaagctc
     5161 ggccccggcg tggatgtgaa ctatatgctg cacacgcaga ataagtgagg ttgatgtttg
     5221 gccgcgccaa tgcctgatgc gacgcttgca gcgtcttatc aggcctacaa atgctctccg
     5281 taggccagat aaggcgttta cgccgcatcc ggcaattgtg atggtcagac agcgattacc
     5341 atttcaccgt catcgacaaa aaccctgccg tctgggcaaa atcatcactc cctttctgcc
     5401 acgccacgct gccgcgcagg gacactcgct gactgatatt gcccgtgact cccactttta
     5461 tttcaccccg tcgcttcacc gcatcgtcac tgatagtgct gccatcttct tcaatttccg
     5521 tcgcatgggg atcgtgataa taattcagat ctaatgttgg tgtgacacca accgcggtac
     5581 gccattcgct gtgtaaaccc agccgcgtct gaatatcatc cccctgcgat tgtgacacgc
     5641 gcgcatggtt agcggcggta aaatcatcct gctgcacgcc ctgataaatc acctgcgcct
     5701 gcggttcaat caccacacca cgccccggta accactgata cccggcttcc agcgaggcga
     5761 taatccccga cgaatggtaa tgatctgtgc catcttcatg ttcagaaaca tcattgctaa
     5821 accacgcgta ctgcaaccag ttatccagcc aggccccctg cttctgatta ccgtgctgat
     5881 accagcttga ggtcagccca acggcataac cgtggttctg gttatcggcg cgagttccgg
     5941 tcatattcga gcggctgtcg ccctgattat cactgtagct accaaccgcc ccaagcatcc
     6001 actcgccatc cgtgccccag cgcccgctaa acaagccgcc gctaagctgc accgtagaag
     6061 tgtcttcctg ctgagccagt tgccctgccg ccgtgtaatg ataacgtccg ccgataacac
     6121 gtaaattcag cgtctgacca tcgccaccag cgtgatcgcg tcgctccatc ataaacgcct
     6181 gatttgccgc ccgcaggtta ttgagataac cgccaacttt ggcattcagc accggctggt
     6241 aagcaggcgt aggggccggg tcgggggttg ggtcaggatc cggtgtggga tcaggatcgg
     6301 gagtcgggtt gggattaggt ggcgatggcg gcgttacttc ttgcgatcgc agataccagt
     6361 cgttgttatc ttccaccagc gtgtagtcat acgctcccat attgacgtag ccgctgcctg
     6421 ccagactgaa ctgcgcattg ttttgaaact gcgtgggatc agctgcgaaa tcaaccactt
     6481 taatgcctgt cgatgtcggc tcaccaatcc ctgtaataga gttaaccacc accgtggtat
     6541 tgccagcagt attaccgttc atcaccaatt gatcgcttac cgagtcatcg ccgtttaatt
     6601 cgctatcgag cagtagcgta ccgccgccgg tataatcacc gtttaccgtc agcgtatcgc
     6661 cagcgacgcc attttgcagg ctaaccgcac cggacgtatt ggtcagatca ccattaaccg
     6721 tcacgtcggc aagataggtc aggctgtcag tttcaaggtt ggtattaagc tcacctgtca
     6781 acgtcacgcc atcggtagca ttcaccagcg tcccatcgcc gttaaacaat gaggcattaa
     6841 gggcgacgga agtatcctgc ccggtcagac gcaaaaccgt accatcgctg atatcgatag
     6901 tgccgctgga agtagcatcg atagactgaa tatcctgatc cgcgccagta acgaacgccg
     6961 ccccatcacc gacccacagt gacgaagcat aaggaaagat atccgcaaca tcgcctttca
     7021 gggtgccttc ttcgacacgt actgccgact gcgttgtacc gctggcggtc agctccagcg
     7081 tacccgcccc cgttttagta aatgtgctac cctcatcctg atgctgcccg ctgctgtcag
     7141 ccatcagtgc gccccactgc gtgtctaccc ccgcatcaac tgccacttca ccgtcggcgc
     7201 gcatttcaat atcacgccca tgctcagtcg tcgtagagcg agtatcgaca ttggcagtaa
     7261 tctccatcac gctttcttgt tgcttatcgg taaagataac ctggcggtta tagtgggtat
     7321 ctccaagctg cgagttgtcg ctcaccatca gcgtaccgga ggcgatttgt gttgtgccca
     7381 aataactatt attgtttgcc agacttacct gcccgtcgct cgcatcgacc accacgtaga
     7441 cgccatcttt cccgtcgccg ccgatatgca aattgtcgcc acctgcaaga tctaccacat
     7501 cctgactgcc gataaccgtt ccgctgctgc cactgacttc aaggccatca ttgtatgatg
     7561 tgccgctctg ccaggtggag aaatcagaga gatcgagcac accgccgttt aacacgatcg
     7621 actgcggatc gtcctggaga gcggcgagat ctgccgcgtc gccttccagc gaaagcaccg
     7681 caccatcatc aacgactata tcgccggtta gcgccatcga ctgcgccccc gacagcacgt
     7741 agctgccgtt ttgcgcaatg gtgagctgac cagcaccttc gatggtgcca gcaaaactgc
     7801 cttgattaac agtaacattg ccaccagcat cgatatttaa agtgccattc tgaaagcccg
     7861 tcaatgagtg cacaaaggtt tgttgggtgg agccaacgtt tagctctgcc tgattctggt
     7921 acttatcaat actccctatc gtcagaccgt agcagtcttg cggatcgtcc tggcaatgcg
     7981 tatcgccgac attcatcagg gagttgctgc ggcccagggt aacttcaccg ttttcaatct
     8041 gcatctcacc agtaaagtca ttgttatctg cattaagtac cagatcgccg gaacctgttt
     8101 tggtgatcaa cccggtacca gcaatagagt caacagctcc gtcattctct gtattgccaa
     8161 taaccagcgt ttttccgtcg gcaatatcaa aggtaacttc acttaagccg agatacataa
     8221 agccacccgc cgcagtggaa ggaccatctc catagcctgc tgcgctgttg ttctcatcca
     8281 ctaacacgcc gccgttctgg ctgtagctgt catcaacaga aatatcaata agatagggag
     8341 ccgtcgcgct attggtatat atcgccccgc cataaccttc ggcagtgtta tttgtaaagg
     8401 cagtgttatt tattatcgta taacctggag gatgcttgct gtcgctatta ttatcggtaa
     8461 catcgattgc cccgccatcg ccatcactgt aacttgttga tgtatatgcc tggttgttat
     8521 caaaaataac atcacttaaa tagacgtcat tattaatggt ataaattgcg ccacctttgc
     8581 catcattagc gatattattg cgaaacatgg cgttagtgac acttaaatcg acggcaccag
     8641 tatcgttagt accagaagaa tagattgcgc caccatagcc gcctgcgacg ttaccggaaa
     8701 aaatgacatc agtaagattc agcgttgagt tttctttagc aaatatggcc ccgccgttat
     8761 aatattctcc tgtaacggtg ttattggcaa acagagtcat tccagtttcg ttttctggtg
     8821 ataatgtaaa ttccgctcct tgttgcaaaa atacggcccc accgctggca ttattggtca
     8881 tatccgaaaa aaccagccat tgcccatcgg cgatactcca gtcctgcgta atgcctgaaa
     8941 ggctttgcct gctggcgtga caactcgctt tgatatcata tccctggcat aaatcaatgg
     9001 ccgccgcgac accgttagca gagaaaagag atgcaatcat tgacgggagt agagataaat
     9061 actccttgcg tagaaagata atccgcatat taatctgacc atcattttat ataagacggc
     9121 atatttaaac gcattataca atatgggtta tctttctttt gtaaattgtc caacccgatg
     9181 caatggctat tttgttatta tttgatgaat aatatcagtg cgtcataatt caagttaata
     9241 accttaaggg ataccagtta tatttaaact aaattatagt aatgaataat tttcttataa
     9301 tataaggtaa attaacaaaa tggcttcgca tttaacaata accgaataga aaacaaccat
     9361 ttcgacatca acaatcgctt acattcgctt atatattgac cacaactgat acatcagatt
     9421 atgtgatgac tcgtgcttag atcaattttt gcaatcatta acaaaaagat taataaacca
     9481 tctatatcaa tttatccaac ctattctgcc gttcaagaaa tcgccgaaca gttattttta
     9541 acaaattttt ctcttcccat tgactttccc ggacaccttg tctgacctaa ggtgcgcgaa
     9601 agccactttt tccttcctga gttatccaca aagttatgca cttgcaagag ggtcattttc
     9661 acactatctt gcagtgaatc ccaaacatac cccctatata tagtgttcta agcagcttcc
     9721 cttactacag gtagtctgca tgaaactatt gcggaaagaa ttccaaaaac aggtacgaca
     9781 tacatgaatc agaatctgct ggtgacaaag cgcgacggta gcacagagcg catcaatctc
     9841 gacaaaatcc atcgcgttct ggattgggcg gcagaaggac tgcataacgt ttcgatttcc
     9901 caggtcgagc tgcgctccca cattcagttt tatgacggta tcaagacctc tgacatccac
     9961 gaaaccatta tcaaggctgc cgcagacctg atctcccgtg atgcgccgga ttatcagtat
    10021 ctcgccgcgc gcctggcgat cttccacctg cgtaaaaaag cctacggcca gtttgagccg
    10081 cctgcgctgt acgaccacgt ggtgaaaatg gtcgagatgg gcaaatacga taatcatctg
    10141 ctggaagact acacggaaga agagttcaag cagatggaca cgtttatcga tcacgaccgt
    10201 gatatgacct tctcttatgc tgccgttaag cagctggaag gcaaatatct ggtacagaac
    10261 cgcgtgaccg gcgaaatcta tgagagcgcc cagttccttt atattctggt tgccgcgtgc
    10321 ctgttctcga actacccgcg tgaaacgcgc ctgcaatatg tgaagcgttt ttacgacgcg
    10381 gtttccacat ttaaaatttc gctgccgacg ccaatcatgt ccggcgtgcg taccccgact
    10441 cgtcagttca gctcctgcgt actgatcgag tgcggtgaca gcctggattc catcaacgcc
    10501 acctccagcg cgattgttaa atacgtttcc cagcgtgccg ggatcggcat caacgccggg
    10561 cgtattcgtg cgctgggtag cccgattcgc ggtggtgaag cgttccatac cggctgcatt
    10621 ccgttctaca aacatttcca gacagcggtg aaatcctgct ctcagggcgg tgtgcgcggc
    10681 ggtgcggcaa cgctgttcta cccgatgtgg catctggaag tggaaagcct gctggtgttg
    10741 aaaaacaacc gtggtgtgga aggcaaccgc gtgcgtcata tggactacgg ggtacaaatc
    10801 aacaaactga tgtatacccg tctgctgaaa ggtgaagata tcaccctgtt cagcccgtcc
    10861 gacgtaccgg ggctgtacga cgcgttcttc gccgatcagg aagagtttga acgtctgtat
    10921 accaaatatg agaaagacga cagcatccgc aagcagcgtg tgaaagccgt tgagctgttc
    10981 tcgctgatga tgcaggaacg tgcgtctacc ggtcgtatct atattcagaa cgttgaccac
    11041 tgcaataccc atagcccgtt tgatccggcc atcgcgccag tgcgtcagtc taacctgtgc
    11101 ctggagatag ccctgccgac caaaccgctg aacgacgtca acgacgagaa cggtgaaatc
    11161 gcgctgtgta cgctgtctgc tttcaacctg ggcgcaatta ataacctgga tgaactggaa
    11221 gagctggcaa ttctggcggt tcgtgcactt gacgcgctgc tggattatca ggattacccg
    11281 atcccggccg ccaaacgtgg agcgatgggt cgtcgtacgc tgggtattgg tgtgatcaac
    11341 ttcgcttact acctggcgaa gcacggtaaa cgctactccg acggcagcgc caacaacctg
    11401 acgcataaaa ccttcgaagc cattcagtat tacctgctga aagcctctaa tgagctggcg
    11461 aaagagcaag gcgcgtgccc gtggtttaac gaaaccactt acgcgaaagg gatcctgccg
    11521 atcgatacct ataagaaaga tctggatacc atcgctaatg agccgctgca ttacgactgg
    11581 gaagcattgc gtgaatcaat caaaacgcac ggcctgcgta actctacgct ttctgccctg
    11641 atgccgtccg agacctcttc gcagatctct aacgccacta acggtattga accgccgcgc
    11701 ggttacgtca gcatcaaagc gtcgaaagac ggtattttgc gccaggtggt gccggactac
    11761 gagcacctgc acgacgccta tgagctgctg tgggaaatgc cgggtaacga tggctacctg
    11821 caactggtag gtatcatgca gaaatttatc gatcagtcga tctctgctaa caccaactac
    11881 gatccgtcac gtttcccgtc aggaaaagtg ccgatgcagc agttgctgaa agacctgctc
    11941 accgcctaca aattcggggt caaaacgctg tattatcaga acacccgtga cggcgctgaa
    12001 gacgcgcagg acgatctggt accgtcaatc caggacgatg gctgcgaaag cggcgcatgt
    12061 aagatctgat attgagatgc cggatgcggc gtaaacgcct tatccggcct acggctcggt
    12121 ttgtaggcct gataagacgc gccagcgtcg catcaggctc cgggtgccgg atgcagcgtg
    12181 aacgccttat ccggcctacg gctcggattt gtaggcctga taagacgcgc cagcgtcgca
    12241 tcaagcacag gatgcggcgt aaaatgcctt atccggcatt aaactcccaa caggacacac
    12301 tcatggcata taccaccttt tcacagacga aaaatgatca gctcaaagaa ccgatgttct
    12361 ttggtcagcc ggtcaacgtg gctcgctacg atcagcaaaa atatgacatc ttcgaaaagc
    12421 tgatcgaaaa gcagctctct ttcttctggc gtccggaaga agttgacgtc tcccgcgacc
    12481 gtatagatta ccaggcgctg ccggagcacg aaaaacacat ctttatcagc aacctgaaat
    12541 atcagacgct gctggattcc attcagggtc gtagcccgaa cgtggcgcta ttgccgctta
    12601 tttctattcc ggaactggaa acctgggtcg aaacctgggc gttctcagaa acgattcatt
    12661 cccgttccta tactcatatc attcgtaata tcgttaacga tccgtctgtt gtgtttgacg
    12721 atatcgtcac caacgagcag atccagaaac gtgcggaagg gatctccagc tattacgatg
    12781 aactgatcga aatgaccagc tactggcacc ttctgggcga aggcacccac accgttaacg
    12841 gtaaaaccgt aaccgttagc ctgcgcgagc tgaagaaaaa actgtatctc tgcctgatga
    12901 gcgttaacgc gctggaagcg atccgcttct acgtcagctt tgcttgttcc tttgcctttg
    12961 cagaacgcga actgatggaa ggtaacgcca agattattcg cctgattgcc cgcgatgaag
    13021 ccctgcacct gaccggtact cagcatatgc tgaatctgct gcgcagcggc gcggatgatc
    13081 cggagatggc ggaaattgcc gaagaatgta agcaggagtg ctatgacctg tttgtccagg
    13141 cggcacagca agagaaagac tgggcggatt atctgttccg tgacggttcg atgattggtc
    13201 tgaataaaga tattctctgc cagtacgttg aatacatcac caatatccgc atgcaggcag
    13261 tcggtttgga tctgccgttc cagacgcgct ccaacccgat cccgtggatc aacacctggc
    13321 tggtgtctga taacgtgcag gttgctccgc aggaagtgga agtcagttct tatctggtcg
    13381 ggcagattga cgcggaagtg gacaccgacg atttgagtaa cttccagctc tgatggcccg
    13441 cgttaccctg cgcatcactg gcacacaact gctgtgccag gatgaacacc cttcccttct
    13501 ggcggcgctg gaatcccaca atgttgcggt tgagtaccag tgccgcgaag gttactgcgg
    13561 ctcctgtcgc acccgactgg tcgcaggtca ggtggactgg attgccgaac cgttagcctt
    13621 tattcagccg ggggaaattt tgccctgttg ttgccgggca aaaggcgata ttgaaatcga
    13681 gatgtgaatt ggttgtagtg ccagatacaa cgcttatacg tcttatctgg cctacaacga
    13741 ttacatggcg tagtaatacg ctttcacctg ctcccagtcc gctttgggga ttggctccag
    13801 atattttcca gctggcggaa gtcatgatta atcgctttat tgcgacgcaa gcggcgacga
    13861 cttttctcca ggtcaagaaa accagcttct gctttacctt ctgttttcac atagatatgg
    13921 cgaacataac aacagccatg ctgacgatta acgctatgca ttttcttaaa cgccagcgcc
    13981 actgctttca acatggcttg ccgtacttcg tcagaatagg gcgatactgc atgccgggca
    14041 taccagtcag caatgctgat gaaccccgcc atatcttcag tcaccagcaa cgcgcgccat
    14101 tcaccctcaa ttttcaccgc ttcaccaaaa acgatcttcg gcacgatgac accggcccgt
    14161 tccagttctt taattaccgc aacctcacgg acaatcgttg gtcggccgaa cggataacgt
    14221 acggaatgaa acagatgatg cgtcatgcgc tttacataca gctttttgcc gttgcgctcg
    14281 acgcattgca ccccgctcat accattacgg cgatagttag gctcttcaac ccagtcgccc
    14341 tctgttgccc accagtgatt aaattcgtcg tactttgctg aaactgccat accctatcgc
    14401 ctgtcatttt tattaacgac aatgactata ggtggttacc tgaggaaaat cttaatgaaa
    14461 cgtgtcgtat taatgaattt cttttgccgc tactagtgtg acaccgcttt tgcccttcat
    14521 ttgcagcgca tagattccac cagccacgac cactaatagt gcgcccagca gataaaatgt
    14581 gaatttaatc aggtggatac agcgatgaat tttattcgac agggattagg catcgcctta
    14641 ttaccagagt taacgctgaa aaccattgca ggtgaattgt gttccgttcc tcacgaacca
    14701 accttctatc gacagatttc gttgttggct aaagaaaagc cggtagaagg cagtccactg
    14761 tttttactac aaatgtgcat ggaacaatta gtggcgattg gaaaaatttg atatgagaca
    14821 ggatggcgca tgaacgccat cctgcagtag cattactctt tattaaggaa ttttactgcc
    14881 ttatcaggga aatcagtaaa cagcccattt acacccgctt tgttatacag agcatcatat
    14941 aactgattca catcagtagt gtattcaggc agtttatccg accgcacggt ataaggatgc
    15001 actaccagtt tattctgctg agcatcttgc accatgccag tgagtttgat attacccggc
    15061 tgcgatgttt cctcaatcaa catatggtaa tccggcccga taccgtcggc atattccgcg
    15121 acttgcttca tggcacctgg cttaaacatc cagtcatagc tgtaattaac ccagcttccg
    15181 tccggctgtt tctgctgcgt ttcattccag tcggtatagg caatcagctg taccagattg
    15241 agatccatgc ccattttagg ttccagctca ttcttaatac gcttcagctc atcagcatca
    15301 aaacattgca aataaacttt atcgtcctta ccggtgtaac catatttctt cagcacttcc
    15361 agcgtttttg ccgcaatatc cttcccttcc tgatgatgga accacggcgc tttgatttct
    15421 ggatagatac cgatattttt cccggtagag tgatttaacc cctgaacaaa ttcaatctct
    15481 tcttcaaagg tgtgcacccg gaagtcggac ttacccattg ggaaacgccc cggataagtc
    15541 tgtacttttt taccgttttc aatatcgaaa ccttcggtaa atttcagcga cttaatttca
    15601 tccagcgtga aatctatcgc gtagtaacga ccgtctttgc gcgcccgatc cgggaaacga
    15661 tcggcaacat cagtaacacg atcgagataa tggtcatgca gaacaaccag atggtcgtct
    15721 ttggtcatca ccaaatcctg ttccagataa tccgctccct gcgcatacgc catcgctttt
    15781 gctggcagcg tatgctccgg caaatatcca ctggcaccgc gatgggcgat gactattttt
    15841 tcgttgctgt ccgccgccat tgcactgctt cccatgacta tagtgctcat catgatcgcc
    15901 atgctaaggt ttttcagctt caatttcatg ccattagcct ccgttgcgtt tttgcagtaa
    15961 ttgttcatgg cgacgttttt cgccaatcat cacaacaatc aacaagataa ccgccagaat
    16021 gctgccgcca atcattacca taaagccgcc atcccagccg aagaagtcca cggtgtagcc
    16081 aacaatcgcg ctcgccgcca ccgaaccgcc caggtaacca aacagcccgg taaagcccgc
    16141 tgccgtacct gccgcttttt tcggtgccag ttccagcgca tgcagaccga tcagcatcac
    16201 aggaccgtag atcaggaagc cgataacaat catacaaatc atatcgacgg ttgggttacc
    16261 tgccgggttc atccagtaaa cgatagtcgc gatggtcacc agtgtcataa agaaaacgcc
    16321 ggttgcccca cggttgccac ggaagacttt atccgacatc cagccgcaca gcagagtgcc
    16381 cggaatacct gcatattcat aaaggaagta ggcccaggag gatttatcca gcgcgaaatg
    16441 cttaacctct ttcagataag tcggtgacca gtcgaggatg ccgtaacgca gcagataaac
    16501 gaacacgttg gcgatggcga tataccacag cagtttgttc ggcagtacgt actgcatgaa
    16561 gatttgcttc gccgtcagct cctgttccgc tttttcgtta tagtcgtccg gataatcatt
    16621 tttgtactct tcgatcggcg gcaagccaca ggattgcggg gtatcgcgca tcatcgcaaa
    16681 ggcgaataat gccaccagaa tggcgcagaa agcaggcata tagagcgccg catgccagtc
    16741 attgaaccag gccatcccca gcaggaacag cagcggcgga ataccaccac cgacgttgtg
    16801 cgcacagttc cacactgaca caatgccgcc acgttctttc tgcgaccacc agtgcaccat
    16861 agtacgacca cacggcggcc accccatccc ctggaaccaa ccgcagagga acaacagtac
    16921 aaacatcacc gcaatgctcg acgtcgccca tggcacaaag cccataaaca acatcactgc
    16981 cgccgccaga atcaaacctg cgggcaggaa aacgcgcgga ttcgagcgat ccgataccga
    17041 acccatgatg aatttcgaaa atccataagc aatcgagatc cccgaaaggg caaaacctaa
    17101 atcaccgcgt gagaatccct gctcaaccag ataaggcata gcaagcgcaa agttcttacg
    17161 aaccaaatag taagccgcat agccaaagaa tatccccagg aaaatttgcc agcgcaatcg
    17221 acgataagtc ggatcgatct ccgcggcagg taagcgcgct ttgtgtggcg ctggtttaaa
    17281 aatactcaac attgatagcc tccgtggccc gtggtcttat ttatgattaa cagcctgatt
    17341 cagtgagaga acctgccgtt tcttgagttg ccgcgatgtt aagaaaacat tcataaatta
    17401 aatgtgaatt gccgcacaca ttattaaata agatttacaa aatgttcaaa atgacgcatg
    17461 aaatcacgtt tcactttcga atcatgagcg aatatgcgcg aaatcaaaca attcatgttt
    17521 ttactatggc taaatggtaa aaaacgaact tcagagggat aacaatgaaa actcgcgact
    17581 cgcaatcaag tgacgtgatt atcattggcg gcggcgcaac gggagccggg attgcccgcg
    17641 actgtgccct gcgcgggctg cgcgtgattt tggttgagcg ccacgacatc gcaaccggtg
    17701 ccaccgggcg taaccacggc ctgctacaca gcggtgcgcg ctatgcggta accgatgcgg
    17761 aatcggcccg cgaatgcatt agtgaaaacc agatcctgaa acgcattgca cgtcactgcg
    17821 ttgaaccaac caacggcctg tttattaccc tgccggaaga tgacctctcc ttccaggcca
    17881 cttttattcg cgcctgcgaa gaagcaggga tcagcgcaga agctatagac ccgcagcaag
    17941 cgcgcattat cgaacctgcc gttaacccgg cattgattgg cgcggtgaaa gttccggatg
    18001 gcaccgttga tccatttcgt ctgaccgcag caaacatgct ggatgccaaa gaacacggtg
    18061 ccgttatcct taccgctcat gaagtcacgg gactgattcg tgaaggcgcg acggtgtgcg
    18121 gtgttcgtgt acgtaaccat ctcaccggcg aaactcaggc ccttcatgcg cctgtcgtgg
    18181 ttaatgccgc cgggatctgg gggcaacaca ttgccgaata tgccgatctg cgcattcgca
    18241 tgttcccggc gaaaggatcg ctgctgatca tggatcaccg cattaaccag catgtgatca
    18301 accgctgccg taaaccttcc gacgccgata ttctggtgcc tggcgatacc atttcgctga
    18361 ttggcaccac ctctttacgt attgattaca acgagattga cgataatcga gtgacggcag
    18421 aagaggttga tattctgctg cgtgaagggg aaaaactggc ccccgtgatg gcgaaaacgc
    18481 gcattttgcg ggcctattct ggcgtgcgcc cgctggttgc cagcgatgac gacccgagcg
    18541 gacgtaacgt cagccgtggc atcgtgctgc tcgaccatgc tgaacgcgat ggtctggacg
    18601 gatttatcac catcaccggt ggcaaactga tgacctatcg gctgatggct gaatgggcta
    18661 ccgacgccgt gtgccgcaaa ctgggcaaca cgcgcccctg tacgaccgcc gatctggcac
    18721 ttcctggttc acaggaaccc gctgaagtta ccttgcgtaa agtcatctcc ctgcctgccc
    18781 cgctgcgcgg ttctgcggtt tatcgtcatg gcgatcgcac gcctgcctgg ctgagcgaag
    18841 gtcgtctgca ccgtagcctg gtatgtgagt gcgaagcggt aacagcgggt gaagtgcagt
    18901 acgcggtaga aaatttaaac gttaatagcc tgctggattt acgccgtcgt acccgtgtgg
    18961 ggatgggtac ctgccagggc gaactctgcg cctgccgcgc tgccggactg ctgcaacgtt
    19021 ttaacgtcac gacgtccgcg caatctatcg agcaactttc caccttcctt aacgaacgct
    19081 ggaaaggcgt gcaacccatc gcctggggag atgcactgcg cgaaagcgaa tttacccgct
    19141 gggtttatca gggactgtgt ggtctggaga aggagcagaa agatgcgctt tgatactgtc
    19201 attatgggcg gcggcctcgc cggattactc tgtggcctgc aactgcaaaa acacggcctg
    19261 cgctgtgcca ttgtcacgcg tggtcaaagc gcgctgcatt tctcatccgg ttcgctggat
    19321 ttgctgagcc atctgcctga tggtcaaccg gtgacagaca ttcacagtgg actggaatct
    19381 ttgcgtcagc aggcaccagc ccatccttac agtcttctcg aaccacaacg cgtgctcgat
    19441 ctcgcttgcc aggcgcaggc attgatcgct gaaagcggtg cgcaattgca gggcagcgtg
    19501 gaactggccc atcagcgcgt taccccgctc ggcactttgc gtgcaacctg gttaagttcg
    19561 ccagaagttc ccgtctggcc gctacccgcg aagaagatat gtgtagtggg gataagcggg
    19621 ctgatggatt tccaggcgca tcttgcggcg gcttcgttgc gcgaactgga tctttcggtt
    19681 gaaaccgcag aaatagagct gccggaactg gatgtcctgc gcaataacgc caccgaattt
    19741 cgcgcggtga atatcgcccg tttccttgat aatgaagaaa actggccgct gttacttgat
    19801 gcgcttattc ctgtcgccaa tacctgcgaa atgatcctga tgcccgcctg cttcggcctg
    19861 gccgatgaca aactgtggcg ttggttgaat gaaaaactac cttgttcact gatgcttttg
    19921 ccaacgctgc cgccttccgt gctgggcatt cgtctgcaaa accagttaca gcgccagttt
    19981 gtgcgccagg gtggcgtgtg gatgccgggc gatgaagtga aaaaagtgac ctgtaaaaat
    20041 ggcgtagtga atgaaatctg gacccgcaat cacgccgata ttccgctacg tccacgtttc
    20101 gcggttctcg ccagcggcag tttctttagt ggcggactgg tagcggaacg taacggcatt
    20161 cgcgagccga ttctcggcct tgatgtgcta caaaccgcca cgcggggtga atggtataag
    20221 ggcgattttt ttgcgccgca accgtggcag cagttcggtg taaccactga tgagacgcta
    20281 cgcccgtcgc aggcagggca aaccattgag aacctgtttg ccatcggttc ggtgctgggc
    20341 ggatttgatc ccatcgccca gggatgcggc ggcggtgttt gtgccgtcag tgctttacat
    20401 gccgctcaac agattgccca acgcgcagga ggccaacaat gaacgacacc agcttcgaaa
    20461 actgcattaa gtgcaccgtc tgcaccaccg cctgcccggt gagccgggtg aatcccggtt
    20521 atccagggcc aaaacaagcc gggccggatg gcgagcgtct gcgtttgaaa gatggcgcac
    20581 tgtatgacga ggcgctgaaa tattgcatca actgcaaacg ttgtgaagtc gcctgcccgt
    20641 ccgatgtgaa gattggcgat attatccagc gcgcgcgggc aaaatatgac accacgcgcc
    20701 cgtcgctgcg taattttgtg ttgagtcata ccgacctgat gggtagcgtt tccacgccgt
    20761 tcgcaccaat cgtcaacacc gctacctcgc tgaaaccggt gcggcagctg cttgatgcgg
    20821 cgttaaaaat cgatcatcgt cgcacgctac cgaaatactc cttcggcacg ttccgtcgct
    20881 ggtatcgcag catagcggct cagcaagcac aatataaaga ccaggtcgct ttctttcacg
    20941 gctgcttcgt taactacaac catccgcagt taggtaaaga tttaattaaa gtgctcaacg
    21001 caatgggtat cggtgtacaa ctgctcagca aagaaaaatg ctgcggcgta ccgctaatcg
    21061 ccaacggctt taccgataaa gcacgcaaac aggcaattac gaatgtagag tcgatccgcg
    21121 aagctgtggg agtaaaaggc attccggtga ttgccacctc ctcaacctgt acatttgccc
    21181 tgcgcgacga atacccggaa gtgctgaatg tcgacaacaa aggcttgcgc gatcatatcg
    21241 aactggcaac ccgctggctg tggcgcaagc tggacgaagg caaaacgtta ccgctgaaac
    21301 cgctgccgct gaaagtggtt tatcacactc cgtgccatat ggaaaaaatg ggctggacgc
    21361 tctacaccct ggagctgttg cgtaaaatcc cggggcttga gttaacggtg ctggattccc
    21421 agtgctgcgg tattgcgggt acttacggtt tcaaaaaaga gaactacccc acctcacaag
    21481 ccatcggcgc accactgttc cgccagatag aagaaagcgg cgcagatctg gtggtcaccg
    21541 actgcgaaac ctgtaaatgg cagattgaga tgtccacaag tcttcgctgc gaacatccga
    21601 ttacgctact ggcccaggcg ctggcttaaa ctcctttctg atgcccggta agcatgtggt
    21661 taccgggcat ttttgcgtac acgattccgt gcccaatgta tgcgttgcaa cgcagtgaaa
    21721 attcctctga aaacgtctcg caaaggctga aactggcaga tgtcaaaggc ctgggataac
    21781 cgtaatgtcg cgtcatcata aatatcaggt gacggacaac catgaccgaa tcaacaacct
    21841 cctccccgca tgatgcggta tttaaaacct ttatgttcac acccgaaacc gcacgggatt
    21901 ttctcgaaat acatttgcca gaaccactgc gcaagctttg caacctgcaa accttacgcc
    21961 tggaacccac tagttttatt gaaaaaagtt tacgcgctta ctactcggat gttttgtggt
    22021 ccgtggaaac cagcgacggt gacggctata tctactgcgt gattgaacat caaagctctg
    22081 cagaaaagaa tatggctttt cggctaatgc gctatgccac tgccgccatg cagcgtcacc
    22141 tggataaagg ctatgacaga gttccgctgg tggtgccatt gctgttttat catggcgaaa
    22201 catcgcccta cccgtactca cttaactggc tggatgagtt tgacgatccg caacttgccc
    22261 ggcagttgta caccgaagct tttccgttgg tggatatcac catcgtacct gacgatgaga
    22321 tcatgcaaca tcggcgtata gctctgcttg aactgattca aaagcatatt cgcgaccgcg
    22381 atttaatcgg tatggtcgac aggatcacca cgcttttggt taaaggcttc actaatgaca
    22441 gccagctaca aacactgttt aattatctgc tgcaatgcgg cgatacctcc cgtttcaccc
    22501 gttttattga ggagatttcc gaacgttcac cactacaaaa ggagagattg atgactattg
    22561 ctgagcggct acggcaggaa ggacaccaaa ttggctggca ggaaggtatg catgaacaag
    22621 ccattaaaat tgctttgcgc atgctggaac agggcattga tcgtgaccag gtgctcgcgg
    22681 ccacccagct aagcgaagcc gatctggcag cgaataacca ctaattaaca caggcccaca
    22741 gccgatcccc atgggccttt gatatcaata actacctttt atgcgtggcc catttttgcc
    22801 tgatttaaac atcgccagtc gttgatccag ggcatcgctg tagagcatcg tgtcaacgcc
    22861 aacagcgaca aagttcgctc cccacgccag gcattgctgc gccatatcag gagccacagc
    22921 cagaaaacca gccgctttac ccgcagcacg gatccgccga atgctggttt caataattcg
    22981 ctgcacttcc gggtgcccgg cgttatccgg gtagcccaac gacgcagaaa gatccgctgg
    23041 tccaataaac acgccatcaa tcccttcgac gtcgaggatc tcgtccaggt tatccagtgc
    23101 cgttttactt tccacctgca ctaacagaca aagcgaatcg ttgacttgcg ccatgtaatt
    23161 ctcaatgcgt ccccagcgcg cagcccgtgc cacactggcc ccaacaccac gctcaccgta
    23221 gggaggatag cgcgtggcag acgccacctg acgagcctga tcggcagtat cgaccatcgg
    23281 gatcaataga gtttgcgcgc caatatccag aacttgttta atcagcgatt tactgccttc
    23341 caccggacgg atcacgggtt ggctggcata gggcgctacc gcctgtagct ggtgataaag
    23401 atcctgaatg gtgtttggcg cgtgctcccc gtcaatcagc aaccagtcat aaccggaagt
    23461 agcggcaatt tctgccatat aagaagtagt cgagcttaac cacagaccaa tttgcacttc
    23521 gcccttgcgt aaacgttctt taaagggatt ggttaataat gcgttcatcg tgttccttaa
    23581 cctatttaat gatgtgccac gtcggtctgc gcaacagtac gatttacacg cagagtgaaa
    23641 ataatcagcg aaccgatgac cgccaccgct gccagtgtca gtaatcctgc cgcatcgctg
    23701 gcaaataacg tttctgcttt cacgcgcagg atcggggcaa taaacccgcc cactgcacca
    23761 aacaggttta caaaaccaat ccccgcagcc agcgccgtac cggaaagcag ttgtgtcggc
    23821 atcgtccaga acaccggctg cacggcaata aacccgatag ctgcaacaca cagcgctacg
    23881 atcgccatta ctggagaaag cagcccggac agaccaatac caatgcccgc cgccagtaat
    23941 gtcagcgctg cgacattacg ccgttcgccg gttttatcgg aatagcgcgg aataagccag
    24001 gtcccaaaca aggccgcaac ccacggaatg gcggtgacca ccgacgctgt aaagcccact
    24061 tttgttccca gcaatgccgc aacctgggtc ggcaggaaga aaattaatcc gtaaaccgcc
    24121 acctgaatgg tcaggtagat aatcgctagt tgccagactc ggccattacg cagcgcatcg
    24181 ctcagccgag aagtcacttt ctgttgttct tcacttgcca gttgattgat aagcaacgtt
    24241 ttttcttgtt tactcagaaa acgtgcctgc tccggtgtgt catcaagcca aaagaatgtg
    24301 aataccccag caccgactgc caacaatcct tcaatcacaa acatccagaa ccagccggga
    24361 tgccccataa atccatgcat ctccaacagc gcgccggaaa gcggtgatcc cagtgttaac
    24421 gccagcggtg cgcccatata gaacagcccc ataatgctgg cgcgattacg ctgcggaaac
    24481 cattgcgagg tgagataaat cataccaggg aaaaatccgg cctccgcagc accaagcaga
    24541 gtgcgaacta tcagaaattt cgcttcagta tcggcccatg ccatggctgc cgaaagaaat
    24601 ccccacagca gtgttgtcgt accaatccag gttctggccc ccagtttgcg catcaaaaga
    24661 ttcgccggaa cacccagaaa cgcatatacc acaaagaaaa tgcctgctcc cagcgcataa
    24721 gcttcattac tcaacccggt atcaatctgg taggtctgtt tggcaaaacc gatattcgaa
    24781 cggtcgagaa acgccagcac atacagcgcc aacataaacg gaattaaacg cacacggttt
    24841 ttcttcacca cggcgtcaag caaagcggtg ctcataaaaa gctccttaga atatgtgggc
    24901 gttaccgttg tcacagcaac gcccggagtg attagtggct gtaggggcgt ttcaaattgc
    24961 agtcacgatt gagttcgacg ccaaaaccgg gtttatcaag cactgattta tgaatacgac
    25021 cattcaccgg aaccggctca ttgagcagaa tcgggtcaaa ctgcggacgc atcgttgaac
    25081 aatccgggct ggtcatcagg aattcgctga atggcgtatt ggtgaaggtg atcaccgcat
    25141 ggtgagagta aacagacgaa ccgtgcggca ccaccagttg ccccctggat ttggcgattg
    25201 cggcaatttc caccagcgtg gttaaaccac cgcaccagcc aacatccggc tgcataatgt
    25261 cgataccggt ttctgaaagc gtacgaaaag attgcagtgt gccgtggtgc tcaccgctgg
    25321 tgaccatcat tccgactggc gcgttgcgtt tcagttcgcg ataaccttca tactgctgtg
    25381 gcggcaggca ctcttcgatc catttcaggt tatagggcgc gcaagcgtgg gccagtttgg
    25441 tggcatagtt cacgtcctga ctcatccagc agtcgagcat taaccagaaa tcctcaccgc
    25501 atttttcacg catatccgcg accataacgg catctttgcg gatccccgca tcgccatcat
    25561 gtggccccca gtgcgtcggc attttgccac cgataaagcc catctctttt gccagatccg
    25621 gacgcgcgcc tgtggcgtag aactgaatct catcacgaac tgcgccgcct aaaagtttat
    25681 aaaccggaag cccgaccact ttgccgaaca gatcccacag agccagatcg acacaggaaa
    25741 tcgtgttcat caccaggcca ccagagccgg agtaatacag ggtggcattg agcatttgat
    25801 catggatcag tttgatatca ctgacacatt taccctcaat gaaacggtta agatgttttt
    25861 cgacaataaa acagcccatt tcaccggctg tcgaaacagc gaatccggtc tggccgtttt
    25921 ctgcttcgac ttcaacaatc aacgtgccaa gaacgttaat gccaaatgac tggcgtgact
    25981 gctcgtaatc gcggtattta ctcatcgggg tggcaatatg atcgtcaatc cagtgattcg
    26041 ccccctggtc gtgataatca ccgccgccag cacctttttc tgctgtcgca ccgccagtaa
    26101 accaggcgcg aacctgtttg atttttggta gggtcatgat gttctccatt gttatgaggc
    26161 ctgtaagtca aagggacttt tccatcccaa cagacgtgaa atatccctgg cgcaggcaat
    26221 ggccttgccc gccagataat cacggtattc ttcattgatt tgtaagcggg taccgaccac
    26281 cgagatcgca gcggtaagct cgttattggc gttaaacacc ggcgcagcga cacaacggac
    26341 atcggggtaa tcttcgccgt tgtcatagct ccagccctga cggcgaatac gcgccagttc
    26401 ttcgtgaagt tgctgtggat gagtaatcgt tgtgggtgtc gcctgctccc agaccagccc
    26461 ttcgataata ctttgctgta ccgctgcagg ttgccaggca agcaggcatt tacctattcc
    26521 ggagcgataa agcgaaaggc tttttccttc atgggaacgc acgctgatag tggctgatga
    26581 ctccactttt aaaatgtaat aagcgctgcc gttatcaata atccccaggt gacataacag
    26641 cccggtggta tccatcagtt gcgtcaaacg cggtcgagcc agttcccgga gatccatctt
    26701 gcttaacgca tgaccagaaa gctccaccag cctggtccac aaacaaaaat tttcctggtg
    26761 atcaaggcta agaaaacgct gacgcctgag ttcattaagc agcaaatagg ccgtgctttt
    26821 agggattccc aatgtgtcaa tgatcgtcgc agcactacag gggccaatgc gggcaatcaa
    26881 attgagaata tcaatagccc gtgtcagggc gggaactttg cttgattcca acatactgga
    26941 ctccagtctt agatactgga atcagtctcg ttgctcaggg ggagtaaaat tgtgaagcgc
    27001 atcaaatccc gctcctcgtc cagcggactg gagaaattac gcacaaattt gtgaatcagt
    27061 gcacggattt tttcttattg agtacaacag gttggacaag ttttgccgga gggggaatgt
    27121 gccggtgcag taaaccggca cagagtggcg gaatcagaca gataaggtca tggactcaac
    27181 aacctcaatc cagccatgct cactggcgat atcctggccg tttaaccaac ggcgcagcat
    27241 attcagtgcc atcattgcgc acacttcctg acggatagcc aggctgtagc gtgtagtgct
    27301 gaaacgcaca cgcagagcga aagtgccgtc tggcgtggct agcgcaaagt tgagatgctc
    27361 gttctcgaaa cccgaaacag ccagtgccag cccggcaaaa tggttggccc gccgttctgt
    27421 aatccagtgc gcagtttgcg ccagggtttc ttcctgtgaa ggaaccactt cacacgccag
    27481 caatggagca cctgcgcgag aaagttgcaa agccaataaa ccaccggtga attgctcgct
    27541 caacgtcagg ctgaactggc ggttttgcaa ttcgcgactg atctgcgcgg gcagtccttc
    27601 ggtgccttcg aaaatcacgc tctgtccggc aacacgttta acgtccagcc acagtttttc
    27661 catcgcctgt tgctcgcttg ccggtccggt gagtttcagt tcgatgatcg gcattgagga
    27721 gcgatagccc attgttacgc ccggcggcag ttgtagagtg tccaggcttt gtgccagatc
    27781 gctttccgaa cgaccaaagg tagttaaacg cagacaaacc ggcgactgtg gtaaagaaaa
    27841 acgctcgcgc aggcgcggca ggatttcgtg ctcgaccatc accttaaatt ctgacggtac
    27901 gccgggagta aagaacatca ggcaacgatt aagccgcacg gcaaaaccac aggcggtgcc
    27961 taccgggtta ttgataaatt cagcactggc aggcagctcc gcttgtttac ggttgctcgg
    28021 tgccattact cgtccacgtt cgtggaaata gcgttccatc tctttgagcc aggcttcatg
    28081 cagcaccagg ccttcacctt ttgctgtcgc agcggcgagt gcacttaaat catcactggt
    28141 cggtcccagc ccgccgttaa cgatcagcac atcggcgtgc tgactacgtt cgcgaagaat
    28201 ggtgactaag tcatcaaggt tatcccccac cgtattgcgg cgagataatg gcaacccctg
    28261 atgaaagaaa aaatcggcca gccaggcagc gttagtgtca acgatttgcc cgtgtaacac
    28321 ttcatccccg gtggataaca tttccacttt taacatggtt atctcctgct tttgttatcg
    28381 aatcactata acgcaaaggg agataaggcg tagtaagaaa ctgaatggcg cgacagaatg
    28441 ccgcgccgga agggatcaga aactggcgtt aaccccaaca tacgggccgt cagccacggc
    28501 gttgtcgcgg ttaccgtctt tacccgacag attcaggtag cgataacccg cctcaatact
    28561 gactggacgc ataatggtgt aacgcgcgcc agcattcgct tcctcataac ttttaatacc
    28621 gctggagagt gaatccggag agtagtaata ctcgccaaac aaacggaagc tgttgccaat
    28681 tttccactgc aaaccacctc ctaccgctgc ggcataacct tcatcgccgt aattcgggtt
    28741 ggtgtaaacg ccttttccgc caacggtcgc cattaacggc ccgagaggca gattcaaccc
    28801 cagccccacg ccagcgacgt caccgtcgtc gtcgttatgt gtccagttac cgcttaacgc
    28861 caggcccgtc gattcagtcc caaaaccgac accaatattg gtgtattctt tacccgcctg
    28921 accgctgatg ctcattgcat tgaccgatgc agaaaccaac agcattccgg ccagacctgc
    28981 tagcgcaatt tttttcatta tcccctttcc cacaagcaat taatcaaaat tcccaaagct
    29041 gcgggattgt actgcctggc acagaggatt caatgagcga aaaaagggat ctagtcactt
    29101 attgtaacta aatacttctt aaataattaa acgccgaaaa aataaacaca ctttaccgat
    29161 acacacaaaa ataataataa gtgcagttat catcgcaaaa gagattactg tcactttcac
    29221 atatttctga gttaccgtat tctcatggtt tctttttatt tataaggaat acccctgtgc
    29281 gacaacggac tattgtatgc cctttgattc aaaatgatgg tgcttatttg ctgtgcaaaa
    29341 tggccgacga tcgcggcgtt ttccccggtc aatgggcgct ttcgggtggc ggcgtggagt
    29401 ctggcgaacg aattgaagag gcactacgcc gcgaaattcg cgaagaactg ggagaacagc
    29461 tgcttttgac agaaatcacg ccgtggacct tcagcgatga tattcgcacc aagacgtatg
    29521 cagatggtcg caaggaagag atttatatga tttacctgat ttttgactgc gtttctgcca
    29581 accgagaagt gaaaataaac gaagagtttc aggactacgc gtgggtaaaa cctgaagatc
    29641 tggtgcatta tgatttaaat gttgccaccc gaaaaacgtt acgtttgaaa ggtcttcttt
    29701 aacaatgatg acagccatca ctggctgtca gattgaaaat ttagtggtta acgaattccc
    29761 catccagata aactttgcct ttttccacat gcatgactaa accatccaga taatcaggtt
    29821 taaatgtcgc atcacgctta tttttagcaa tatatgtcag gcaatgatta tgggtgaaaa
    29881 taacgatatt tttatcaggc gctttgcttt gtaagtcctt aattgcacta taaatctcat
    29941 taccgcactg aagaagtcgt ttatctaccg tcagtttttt acccgcggaa aaccaggtcg
    30001 ccgactgaat ggtccggacg gtattactgg aataaagatc gaaatcaggg atgtcagcgc
    30061 taaaagcgtt gcccagttca cgggcatcct gggtaccttt aaccgtaata cctgttttat
    30121 ctgacaagca ttgattggtt gaacgatcgc aacgttcagc atggcgaaac aaaacgacaa
    30181 ccgggtgctg ctgtgccagt ctggccaatg ttttattgtc gatacgtggc aaaccattgc
    30241 tactccaggc ggcatgagta cccagtccgg caattgcagc gagcgccagt aaaatgatga
    30301 aatatttttt tgacttcaat gatgagcggc aaaaagctaa cacgtaaact ccacctatag
    30361 acaagcgcaa ccagacaatt accgtgaaat tgagcgacat ttctggcgat aattagcagt
    30421 tggtgtaata ttaaaaatcc tacgatgtcg gcaaaatgcc tcaaaatttt gccaaatgca
    30481 aagcctaaat aagaaaaaat ataaaaattt caatatttac gtctaatatt agtttcttaa
    30541 ggttaagtta atattctatc cttaaaattt cgctccaaat ggcaaaatat acacaacact
    30601 ctttatagca aatataagtg gacaggtatt caatggcgga aggaaaagca atgtcagaat
    30661 ttttgccttt ttcgcgacca gcaatgggcg tggaggaact cgctgcagtt aaagaggttc
    30721 tcgaatccgg ttggatcaca accggtccga agaatcaggc gcttgagcaa gccttttgcc
    30781 agttgacggg aaatcagcat gccatcgcgg tcagttcagc caccgccgga atgcatatca
    30841 cgctaatggc gttggaaatt ggcaagggcg atgaagtgat tacgccttcc ctgacctggg
    30901 tttcaaccct caatatgatt tccttgttgg gtgcaacgcc ggtaatggtg gatgtcgacc
    30961 gcgatacgct gatggtcacg cctgaagcga tcgagtcagc cattacgcca cgcactaaag
    31021 ccatcattcc ggtgcattat gccggtgcgc cagcagatat tgacgccatt cgcgccattg
    31081 gcgaacgtta cggcatcgca gttatcgaag atgctgccca tgccgtcggt acgtattaca
    31141 aagggcgaca tattggcgca aaaggtaccg ctattttttc atttcatgcc attaaaaata
    31201 ttacctgtgc ggaaggtggc ctgattgtaa ctgataatga aaaccttgcc cgccagctac
    31261 ggatgctgaa atttcacggt ctgggtgtcg atgcctatga cagacaaacc tggggccgtg
    31321 caccgcaggc tgaagtctta acaccgggct ataagtacaa tctgaccgat attaacgccg
    31381 cgattgccct gacacagtta gcaaaattag agcacctcaa cacccgtcgg cgcgaaattg
    31441 cccagcaata tcagcaagca ctggcagctc tcccctttca gccattaagc cttcccgcct
    31501 ggccgcacgt tcacgcctgg catctgttta ttattcgtgt cgatgaacaa cgttgtggta
    31561 tcagtcgcga tgcgttgatg gaagcgttaa aagaaagagg cattggtacc gggttacatt
    31621 tccgcgccgc tcacacacaa aaatattatc gcgagcgttt tcccacgctg tcgttaccga
    31681 ataccgaatg gaatagcgaa cgcatctgtt ctttgccgct gttcccggat atgactaccg
    31741 ccgatgccga ccgtgtcatc acagcccttc agcaactcgc aggacaataa gccatgtttg
    31801 aaatccaccc tgttaagaaa gtctcggtgg ttattcccgt ttataacgag caggaaagct
    31861 taccggaatt aatcaggcgc accaccacag cctgtgaatc gttggggaaa gagtatgaga
    31921 tcctgctgat tgatgacggc agtagcgata attccgcgca tatgctggtc gaagcctcac
    31981 aagcggagaa cagccatatt gtgtctattt tgcttaaccg caattacggg caacattcag
    32041 cgattatggc gggattcagt cacgttactg gcgacttaat tattaccctt gatgccgatc
    32101 tccagaatcc gccagaagaa atcccccgcc tggtggcaaa agccgatgaa ggttacgacg
    32161 tggtagggac tgtacgccag aaccgccagg acagctggtt tcgtaaaaca gcttcgaaga
    32221 tgattaacag gcttattcag cgcaccaccg gcaaagcgat gggtgactac ggttgtatgc
    32281 tgcgcgccta tcgccgtcat attgtcgatg cgatgttgca ctgccatgaa cgtagcacct
    32341 ttatcccgat tctggcgaat atcttcgccc gccgtgccat tgaaattcca gtacatcatg
    32401 ccgagcgtga gtttggtgaa tccaaataca gttttatgcg cctgattaat ttgatgtacg
    32461 acctggtgac ctgccttacc acaacgccgc tacgtatgct gagtctgctc ggcagcatta
    32521 ttgcgattgg aggttttagc attgcggtgc tgctggtgat tttacgcctg accttcggac
    32581 cacaatgggc ggcagaaggc gtctttatgc tatttgccgt gctgtttact tttattggcg
    32641 ctcagtttat cggcatggga ttgctcggtg aatatatcgg caggatctac accgatgtcc
    32701 gcgcccgccc ccgctatttt gttcagcaag ttatccgtcc atccagcaag gaaaatgaat
    32761 aatgaaaacc gtcgtttttg cctaccacga tatgggatgc ctcggtattg aagctctgct
    32821 ggctgccggt tacgaaatta gcgccatttt tacccatacc gataatcccg ctgaaaaagc
    32881 cttttatggt tcggtggctc gtctggcggc ggaaagaggc attccagttt atgcgccgga
    32941 taacgttaat catccgctgt gggtggaacg cattgcccaa ctctcaccag aggtgatttt
    33001 ctctttttat tatcgccatc ttatttgcga cgagattttg cagctcgctc ccgcaggtgc
    33061 atttaatctg cacggttcgc tgttaccaaa atatcgtggt cgcgcgccgc tgaactgggt
    33121 gctggtgaac ggtgaaacgg aaacgggcgt tacattgcac cgaatggtaa aacgtgccga
    33181 tgctggggcc attgtggcgc aactgcgcgt tgccattgcg ccagacgata tcgctattac
    33241 gctgcatcat aaattgtgcc atgccgcgcg ccagctactg gaacagacat tacccgccat
    33301 taaacacggt aatattctgg aaattgccca gcgcgaaaac gaagccacct gttttggtca
    33361 cagaacgcca gatgacagtt tccttgaatg gcataaaccg gcatccgtac tgcacaacat
    33421 ggtacgtgcc gttgccgatc cgtggccggg tgccttcagc tatgttggca atcagaaatt
    33481 caccgtctgg tcgtcgcgtg ttcatcctca tgccagcaaa gcacagccgg ggagcgtgat
    33541 ttctgttgcg ccactgctga ttgcctgtgg cgatggcgcg ctggaaatcg tcaccggaca
    33601 ggcgggcgac ggcattacta tgcagggctc gcaattagcg cagatgctgg gcctggtgca
    33661 aggttcacgc ttgaatagcc agcctgcctg caccgcccga cgccgtaccc gggtactcat
    33721 cctcggggtg aatggcttta ttggcaacca tctgacagaa cgcctgctgc gcgaagatca
    33781 ttatgaagtt tacggtctgg atattggcag cgatgcgata agccgttttc tgaatcatcc
    33841 gcattttcac tttgtcgaag gcgatatcag tattcattcc gaatggattg agtatcacgt
    33901 caaaaaatgt gatgtcgtct tgccgctggt ggcgatagcc acgccgattg aatatacccg
    33961 caacccgctg cgcgtatttg aactcgattt tgaagagaat ctgcgcatta tccgctactg
    34021 cgttaagtac cgtaagcgaa tcatcttccc gtcaacttca gaagtttatg ggatgtgtag
    34081 cgataaatac ttcgatgagg accattctaa tttaatcgtc ggcccggtga ataaaccacg
    34141 ctggatttat tcggtgtcaa aacaattact tgatcgggtg atctgggcct atggcgaaaa
    34201 agagggttta cagttcaccc tcttccgccc gtttaactgg atgggaccac gactggataa
    34261 ccttaatgca gcgcgaattg gcagctcccg cgctattacg caactcattc tcaatctggt
    34321 agaaggttca ccaattaagc taattgatgg cggaaaacaa aaacgctgct ttactgatat
    34381 ccgggacggt atcgaggcgt tgtaccgcat tatcgaaaat gcgggaaatc gctgcgacgg
    34441 cgaaattatc aacattggca atcctgagaa cgaagcgagc attgaggaac tgggcgagat
    34501 gctgctggcg agcttcgaaa aacatccgct gcgccatcac ttcccaccgt ttgcgggctt
    34561 tcgtgttgtc gaaagtagca gctactacgg caaaggatat caggacgtag agcatcgtaa
    34621 accgagcatc cgcaatgccc gccgctgcct ggactgggaa ccgaaaattg atatgcagga
    34681 aaccatcgac gaaacgctgg atttcttcct gcgcaccgtt gatcttacgg ataaaccatc
    34741 atgaccaaag tcggcttacg cattgatgtc gatacctttc gtggcacccg tgaaggcgtg
    34801 ccacgtctgc tggaaatctt gagtaagcat aatattcagg ccagcatttt tttcagcgtc
    34861 ggcccggaca atatgggccg ccatctctgg cgactggtga agccacagtt tttgtggaag
    34921 atgctgcgct caaacgcggc atcgctttat ggctgggata ttttactggc aggtacggcc
    34981 tggccaggta aagagattgg tcatgccaat gccgatatca ttcgtgaagc ggctaaacat
    35041 cacgaagtcg gcctgcacgc ctgggatcac catgcctggc aagcccgtag cggtaactgg
    35101 gatcggcaaa caatgatcga cgatattgca cgcggtcttc gcactctgga agagattatc
    35161 ggtcaaccgg taacctgttc tgccgctgcg ggctggcgtg ccgaccagaa ggtgatcgaa
    35221 gcaaaagaag cgttccattt gcgctacaac agcgattgtc gtggggccat gccgttccgt
    35281 ccactgctcg aatcaggaaa ccctggcact gcgcaaattc cggtgacctt acccacctgg
    35341 gatgaagtga ttggtcggga tgtgaaagca gaagatttta acggttggtt actcaaccgc
    35401 atcctgcgag ataaaggcac gccggtttat accattcatg cagaagttga aggctgcgct
    35461 tatcagcata attttgtgga tctcctcaaa cgcgcagctc aggaaggcgt gacattttgc
    35521 cctttaagcg aactgttatc agagacgttg ccgctcggac aagttgttcg cggaaatatt
    35581 gccggacgtg aaggctggct gggttgccaa caaattgcgg gtagtcgctg atgaaatcgg
    35641 tacgttacct tatcggcctc ttcgcgttta ttgcctgcta ttacctgtta ccgatcagca
    35701 cgcgtctgct ctggcaaccc gatgaaacgc gttatgcgga aatcagtcga gaaatgctgg
    35761 catccggcga ctggattgtg ccccatctgt tagggctacg ttatttcgaa aaacccattg
    35821 ccggatactg gattaacagc attgggcaat ggctatttgg cgcgaataac tttggtgtgc
    35881 gggcaggcgt tatctttgcg accctgttaa ctgccgcgct ggtgacctgg tttactctgc
    35941 gcttatggcg cgataaacgt ctggctctac tcgccacagt aatttatctc tcattgttta
    36001 ttgtctatgc catcggcact tatgccgtgc tcgatccgtt tattgcattc tggctggtgg
    36061 cgggaatgtg cagcttctgg ctggcaatgc aggcacagac gtggaaaggc aaaagcgcag
    36121 gatttttact gctgggaatc acctgcggca tgggggtgat gaccaaaggt tttctcgccc
    36181 ttgccgtgcc ggtattaagc gtgctgccat gggtagcgac gcaaaaacgc tggaaagatc
    36241 tctttattta cggctggctg gcggttatca gttgcgtact gacggttctc ccctggggac
    36301 tggcgatagc gcagcgggag cctgacttct ggcactattt tttctgggtt gagcatattc
    36361 aacgctttgc actggatgat gcccaacata gagctccgtt ctggtactac gtgccggtca
    36421 tcattgccgg tagcctgccg tggctgggat tactccccgg tgcactgtac acaggctgga
    36481 aaaaccgcaa gcattccgca accgtctatt tgttgagctg gacgataatg ccgctgctgt
    36541 ttttctccgt cgctaaaggt aaattgccca cctatattct ttcctgcttt gcatctctgg
    36601 caatgctgat ggcgcattac gctttgctgg cagcaaaaaa taatcctctg gcgctgcgga
    36661 ttaatggctg gattaacatc gcttttggct tcactggcat tattgccaca tttgtggtct
    36721 ccccgtgggg accaatgaac acgccggtgt ggcaaacctt cgagagctat aaagtctttt
    36781 gtgcctggtc gattttttcg ctatgggcat ttttcggctg gtacacctta acaaacgtcg
    36841 aaaagacctg gtcttttgcc gcgctttgcc cgctggggct ggcgttgctg gtaggatttt
    36901 caattcctga cagagtcatg gaaggaaaac atccgcaatt ttttgtcgag atgacacaag
    36961 aatcacttca gccaagccgc tatattctta ctgatagcgt cggtgttgcc gcaggtctgg
    37021 catggagcct gcaacgcgat gacatcatca tgtatcgcca gacaggtgag ttgaaatacg
    37081 gccttaatta tccggatgcg aaagggagat ttgtcagcgg tgatgagttc gcaaactggc
    37141 ttaatcaaca tcgtcaggag gggattatta cactcgtgct ttcggttgac cgcgatgaag
    37201 atatcaacag tctcgccatt ccgcccgcag atgccatcga tcgtcaggag cgtctggtgc
    37261 tgattcagta tcgtcccaaa tgatctggct aacattagtc tttgccagct tgcttagcgt
    37321 tgccgggcag ttgtgtcaga aacaggcaac ctgctttgtg gcgataaaca aacggcgcaa
    37381 acatatcgtg ctgtggctgg gactggcgct ggcttgtctt ggtcttgcca tggtgctctg
    37441 gctgctggtc ttgcagaacg taccggtagg cattgcctac ccgatgttaa gtctgaattt
    37501 tgtctgggtg acgctggctg cagtaaaact gtggcacgaa ccggtatcgc cgcgtcactg
    37561 gtgtggggtg gcgttcatta ttggcggcat tgtgatcctc gggagtacgg tgtaatgggc
    37621 ctgatgtggg gattattcag cgtcattatt gcctcagttg cgcaattaag cctgggtttt
    37681 gcggcgagtc atctgccgcc gatgacgcac ttatgggatt ttatcgcggc tctgttagct
    37741 tttggcctgg atgcccggat actgttactc gggctgctgg gttatctgct ttccgttttt
    37801 tgttggtata aaacgctaca taaacttgcg ctaagcaaag cctatgcctt gctcagtatg
    37861 agttatgtgc tggtatggat tgcttcaatg gtacttcccg gctgggaagg gactttttcg
    37921 ctgaaagcac tactgggagt agcctgtatt atgagcgggt tgatgctgat tttcctgccc
    37981 acgacaaaac aacgttactg agttttccct gccactttac actgccgttc ccactcatcg
    38041 gggctataac tgcttgccga gaggactttc accgtatgca gcttttcacg attaatacaa
    38101 taaagcgcat tctgcaaagg cgagagtaaa tcccccactt tcacggcgaa gtcactcttc
    38161 acttcggcga tcatttttat cgcgccgcca gcatcgcaga gcataagtac atgtctgtta
    38221 tcttgtttat tgcagcacga ttttttgacc agccattcca ttgcattatc ctgtttgcta
    38281 agagttttca catcaattta cagattaaca gataaaccta ttatcaacgg caatggaatg
    38341 gcaaaattgc gaagtgaaac taatgatttc ttaatcctgg cgttgcaccc actcttttaa
    38401 cgcctgacgt gaaattttaa taccgccgtt tttcagctcc ggcggtaaag ttagccagcg
    38461 caccggttgt tgaaaacgag ccagcttatc tttcacccat tcgccaagat caacggtttg
    38521 ctggtcatac tccaccaccg ctaccggtcg atgaccaaac tccttgtcgg caacggggac
    38581 gataaacacc tgcaaaaccg caggatgtgc agcaattacg cgctcgactt cttccggctg
    38641 aataccctct ccgccactga agaacagatt gtctaaacgt ccgacaatgg tcagcttgcc
    38701 attatgcatc tcaccgcgat cgcgcgtagc gtaccagcct tcgtcattaa ccagtgaaac
    38761 cagttgcccg ttacgccagt aaccttctgc catactggca gcccgcagcc acacttcatt
    38821 attaacgatt ttcacttccc gacccggcag cggcgaacca acgtctgcca ggccgtcggc
    38881 ttctttcgca cacaccgtgg aggcaaactc ggtcagacca tagccgcaaa agcaacgaat
    38941 cccctgctcg cgcgcctgtt ccgtcaactc gaccgggata gccgcgccgc caagtaacac
    39001 cgctttcagg gaaacggaac tgcggttaac cagcaaacgc cagagttgtg ttggcaccag
    39061 tgaagcgtga gtacagcctg ccagcatttg ctccaatggc tgtttatcac gcaccgtcat
    39121 ccgcgcacca gcgtataacc agcgccacat aattccctga ccggagacgt gaaacagcgg
    39181 taaagagagc aaccagtcat cgtgatcgcc aaacggaatc agcgataaca caccttgcgc
    39241 actggcaaga tgggcctgat aagtatggac agcggctttc ggcaaaccgg tagaacccga
    39301 ggtcaacgtc attgagcaca gacgcgtcgg ctgccacgta gcggcatgtg cgccttcaac
    39361 ccgctgaatg tgcagcgacg ttaatgccgg aaacgtgttt tccccatccg gcaccagcgc
    39421 aaattgcagc gtcagattgg gcagcaattc ttcaagcaac ggttgcggca gctgagggtt
    39481 cacgggcaac acccgcgccc cgcattgcag taacgccagc caggcgagca gcgtttgcgg
    39541 cgtattccac gcccgcaaca tcacgccgct gccctcaacc acaccctgcg ccgcaaagcc
    39601 cgtagctaac tcatcgacgc gagcacaaag ctcgcgccag ttgagttgct cgtcattaag
    39661 acgtaaggcg atggcttctc cccgcacttg ccgccagtga cgccacggcc agtcagagaa
    39721 gatcataaca actgctccag tacatcagcg tccactaacg gcagtgggct ccccggccag
    39781 cgacgaacct gctgcgcctg catcagatcc agcgtgtcca gtcccggaat ggtgtctggc
    39841 gttaaccagg cagcaatccg cgccagttgc gttagaccta agctcgactc aatggaagaa
    39901 ctgatcaccg ccgtcagccc cagcgcatgc gccgcctgta cctgctcgcg tactttttcc
    39961 agactgcccg tgagcgtggg tttgataact accgcgcgca cgccctcttc agccacaaag
    40021 gcaaaatccg gctcgcgcag gctttcatcc caggcaatgg caatgccggt ttcacgggca
    40081 aacgctcgcg aatcatcgcg ggttttgcac ggctcttcga gaaacgcgat gcggtggcga
    40141 taatccgggt taacatattt ggcaaactgc tgacctttca gcggtgtcca ggcgcgattt
    40201 gcgtcaagac gcaaatgcag atccggaatt gcctccagca acagattaac caccatgccg
    40261 tcgcgcacgg cttcgtacaa tcccactttg accttcgcca ctttctcgcc tggcatatca
    40321 gcaagtttga ggatcagatc gtcaggatcg ccattacaca gtggtgccgc acggtaattg
    40381 gccgcctgcg ggagcgtatc tgccagctcc gccaatgcac agcttacgcc aaaggcaacg
    40441 gaaggcatct gcggtagctc gccatcgcct gccagccagt tatttaccca ggcaagcagc
    40501 acaatttgcg cctcttccca ggtttcctga ctgaagcccg gcagtgggga gatctccccc
    40561 cacccttcgc gctcgccttc acgcaggcaa acatacagcc cgtcgcgggt ttttaaccgc
    40621 ctgtcgcgca gaaccatccc cgcgtccatg gggatctgcc agcggtatac ctgcgcgcta
    40681 cgcattacgg attccgttta aatttgctga aatcaggctg acgtttctgg ttgaaagcgt
    40741 tgcgaccttc ctgaccttct tccgtcatgt agaacagcat ggtggcgttg cccgccagct
    40801 cctgcagacc cgcctgaccg tcacagtcgg cgttcagcgc agctttcagg cagcgcagcg
    40861 ccatcgggct gttttgcagc atttcgcggc accaacggac ggtttctttt tccagatccg
    40921 ccagcggtac cacggtgttg acaaggccca tatccagcgc ctgttttgca tcgtactgac
    40981 ggcacaggaa ccagatttca cgcgcttttt tctgtccgac gatgcgagcc atgtaggaag
    41041 cgccccagcc gccgtcgaag gaaccgactt tcgggccagt ctgaccgaag atggcattat
    41101 ctgccgcgat agtcaggtcg cacatcatgt gcagaacgtg accaccgccg atggagtagc
    41161 cagccaccat cgcgacaacc ggtttcggac aggtacggat ctgacgctgg aagtccagca
    41221 cgttcaggtg atgtacgccg gaatcatctt tatagccgcc gtaatcacca cgcactttct
    41281 ggtcaccacc ggagcagaac gctttatcgc ctgcgccagt cagaatgatc acgccgacgt
    41341 tgtcgtcata acgcgcatct gccagtgcct ggatcatctc ttttaccgtc agaggacgga
    41401 aggcattgcg cacctgcgga cgattaatgg tgatttttgc gataccgtcg gtggattttt
    41461 cataacgaat gtcctcaaaa ccttcggagc agtcgtgcca ttcaaccggt gcgtaaagca
    41521 ttgcttcatc aggataaatc atattgtgtc ctttagtcag aaacgcaaga tctgcgccag
    41581 acttgcgata acgccagcgg gattttcccg atgcgcgtta tgtccggcgc gaggaatgac
    41641 atggcagtcg gcagccagtt ccgccgccaa ggcgcggaat ttgctgtcac gttcaccaca
    41701 taaataataa aacgcaaatg tgcgggcgct aaggttagca cgtaaatcag gctggacggc
    41761 gagagaagtc gcctccagca tggcggcaag ggttgcgcca ttattgttac tgcgcagcgc
    41821 caccagctcc cggcgttgat cgtcattgag cgaggcaaaa acaggctgtt gataccagtc
    41881 ggcaaatacc gccgttaacg gttctgtgcg aaaacgctgc gcccattggc gatcggaacg
    41941 ctgacgttcc gcgcgttgtt cagcattttg caacccagga tgcccgcctt cgacaacaac
    42001 cccacaaagc cccggcatcc cctggcaagc cgccatcatc gccacccgtc ccccaagcga
    42061 gtaccccacc aaccagaagt taaggatgtt gtaactaacc agggttttac gcagtaagtc
    42121 ggtgacatca tcaaatccat cgacgctaat cgccgccgaa ccaccgtgac ctgggagata
    42181 aacatacaac cgtgagtagt cggcaaacgc ctcgcccact tcttgccatt cgtggcaatc
    42241 gccggaaaaa ccgtgcaaaa acaccagcca gggtaaacct ggtttgccgt gttttgcctg
    42301 cgcgtgcagg atcataaatg gcttacctgc gccagaagtt gctggagcgt ttgcgcacca
    42361 tcggtgtcgt taaccaccat ttcaattacc gtggtggttg gcgtgcgcca ggcatcggca
    42421 agtgccgttt caagttcctg ccagttttgc ggacgatgat atttcagctc gaacatcgcg
    42481 gcggcgtgct caaaatggac gttttgcggc atcagataga aacgctcgcg ctcgcttttc
    42541 ggcgttggca gcagcgagaa aatttgcccg ccgttgttgt tcaccacaat taatatcagc
    42601 ggcgcggaaa cctgacgcaa taacgccagc gcgttgagat cgtaaagtgc ggagagatcg
    42661 cccacaatcg ccagcgtcgg tttgccgctt gcccgctgaa cgccggcggc agtcgagagc
    42721 agcccgtcga taccgctggc cccacggttg ctgtacaccg ggtaacctgc cggaagttgc
    42781 gaaagcgcat caatcagacg taccaccagg ctgttaccga caaacaattg cccctgttca
    42841 ggcagatagt cgctgatgcg atgcgccagt tgcgcttcgc caaacgcatc gcgacgggca
    42901 ataaccgcct gcattgcctg ttccgccagg cgcgggattt caacgcacca gggctggcgt
    42961 ttttctgccg gatgcagctc cagccagtcg gcaatattgg caattaagcg gcgtccacga
    43021 tggtgcgccg gatcaagccg cccttcgatg tcatccacaa tccagtactc ttcgggttca
    43081 cagcttgcct gccattgcag gagccgtttg cccgtcaggc tgcttcccag ttgcaccaca
    43141 atttgcgcct gttgcagttc gctggtcgct ttggcattgc ctaaccagag atcggcacac
    43201 ggcagcggct gtccggtttg cgacagcacg tcgccaatca gcggccagcc aagagtttgc
    43261 gcccacagtg caactttttt gccctcttcc gcactcatgc gcccggcaac caccacgccg
    43321 cgtttttgtc gccagaagaa ccagtcgcgc tgtttttcac tttccagacg aggcgcttca
    43381 cgcagccacg gtttgtcgtc ctgccaccag tcgcccagcc gctgttgcca gctaagcccg
    43441 gtgtcgtcca tttcgccata cagcggttcg gcaaacgggc agttgatatg gactccacca
    43501 gaatgaagcg taccgagagc atggtcgatg gtagaaacca gccaacgtgc ggggatatcc
    43561 tgggtcgggc gcggcaatga aatactgtgc gtggggtgag aggcgaacat tcccggctga
    43621 cgaatcgcct gattcgcgcc gcagtcaatc agctccggcg ggcgatcggc ggttaacagg
    43681 atcagttttt ctccggttaa cccagcttca atcagtgccg gatagagatt tgctaccgcc
    43741 gtaccggagg tcacaatcac cgccaccggc tgcttgctga ctttcgccag ccccagcgcc
    43801 agatgcccca acccacgctc atcgaaatgg gtgtggtgaa tgaatgcgga attctccgcc
    43861 gccgctaacg ttaacggtgt agaacgcgag ccaggggcga tacagatgtg tctgacgccg
    43921 tgacgcgtta atgcttccag aatgaccgcc gcccagcgtc ggttaaatgc gcttactgac
    43981 atgagtttgt ccggtatcaa tattgcggct aagtataagg agctacaaaa atagattatt
    44041 gatatgaatc ggtaatgatg cgactcatta ctattccatc tgtaataaag tacgcagccc
    44101 tgccgctttg ttgtcgattt cctgccactc ttgctcgggg tcggaaccac ggacaatgcc
    44161 cgcgccagca tataatcgca cgacattgcc gctaattttt gctgagcgca gggaaacgca
    44221 gaattcgctt tgctgtaatg agagatagcc cgcagaaccg gcgtaccatt ctcgggtgaa
    44281 cggttcgtga cgggcgataa actgtcgcgc cagatcgcgc ggtaagccag caactgctgc
    44341 cgttggctgc aactgatgta aacagatcac attatccgct ttgttgagtg aagtccagat
    44401 acagcggcga agatgctgca ctttacgcag acgcagtacc tgcggcggta aaacatccag
    44461 cgtctgggta tcggcctgta accgttggca gatatcttcc accaccagca tattctcgcg
    44521 ctggttttta tcatccgcca tcagccactc acctaactgc tgcgcctgct tatcatcagg
    44581 atgatttgct accgttcccg ccagcgcttc agtacgcagc gctttgtcac gccgccgcca
    44641 taaccgttcc ggtgaagagc caagaaaagc attttcgcca tcaaaggcca tgtaaaaatg
    44701 gtagcaattc agattcagtc gacgactggc agccatcatc gccgccgcgt tgaccggact
    44761 tgcgaaatgc aggtcagttg cccgagcgag caccactttg tcgagctcac cttcggcgat
    44821 ggttttcgtt gccagttcga ttaattgcgt ccagcccgtt ttgtccggcc agtgttgttc
    44881 tcgcgtggtg gttaaatgta acccaggcaa gggcttgata ctcaccagtg tggcgataaa
    44941 ttctcttgcc ttaatcgcat cgtgctgaag ggagctttcg ctgaatagcg tcagccgcag
    45001 cgtggcttta ccgccacagc gtcgccattc caggcgtggt aaaagtaaat tgccctgcga
    45061 cgggtcaaac gcattcagcc cccaaatgcg taagtcggcg tgttccgggt gctggcgaag
    45121 aaaacgttgt gcctggtcca acgacgtaaa acgggtaatc gcgcccagga cggcagcttc
    45181 ttcatcacca ttacgttgtt gccagtagaa ttgcgggtat gtttgctgac tggccagcca
    45241 gctcaaggca tcaaaagcgt ctttgagagg gaaaggaata tcgataaccc gaatgccggg
    45301 tgtcgccgga atctcttgcg acaaatggcg cagtagattt tccagcgccg tagtaagtga
    45361 ttgcacgcgg acctctccct gttaaaagcc tcacattata cggggtacta caaaaaaaat
    45421 gcagtacccc ggtgtaggga ggttttaacg gcgtgccagc aacagtccta gtaccagccc
    45481 aacggccgcg cccacaccaa ttccttgcca gggtttttcg tggacgtagt catcagcacg
    45541 ataaactgcc tgcttcgccc gatagtaata actgtctgaa gcctggctaa cccgtttttt
    45601 cacatcatcc agcgcttttt ctgcacgcgc tttcagctca acatatttct gatcggcggg
    45661 atcgccagag gagcggagca cctcttccag tgtttcacta agcagcgtca ggtcgtcatc
    45721 gatacgtgta tcaccaaact gattagacat tctcgttctc catttgcgta aaacctgtca
    45781 ctaactatag acaagggttg ttgattacgc ctgaattacc tcgcgtgcca tcccaatgtg
    45841 tgggatacca tcttcttcat agacctccgt cacagggata aaaccgaaac tctggtagaa
    45901 gttttgcaga tgcgcctgcg ccccgaggta aacaggctta tcaggccagt gatgcgtgca
    45961 ggtgtcgagg gttttactca tcagttgctg ccccactttt tcgccacgca acgcttcact
    46021 gacaatcacc cgaccaataa cgaccggctc aagatcatca tcacttttca gaatcctcgc
    46081 atatgccacc agttcatcat ttttccaacc gagaatatgg cgattatccc ccgtcaaatc
    46141 atcaccatcg atatcctgat aagggcaatt ctgctcgaca acaaacaccg cgcaacgcag
    46201 ttgtaataag gcataaagct gggaaaccga taattcagag tgatgcagat cttgccattc
    46261 aatcataatg ctctcctttt agatgtatgc cttatactag ccggaatatt ttttgaaacg
    46321 tgatgaactc atggaattaa tttttttagg tacttcagcc ggtgtgccaa cccgcacgcg
    46381 caatgtcacg gcaatattgc ttaacctgca acatccgacc cagagcggac tttggttgtt
    46441 tgactgcggt gaaggcaccc agcatcagct actgcatacc gcctttaacc ctgggaaact
    46501 ggacaagatt tttatcagtc accttcatgg cgatcatctc tttggtttac ccggcttgct
    46561 gtgcagtcgt tctatgtcag gcattatcca acccttaacg atttatggtc cgcaaggtat
    46621 ccgtgaattt gtggaaaccg cgctgcggat tagcggctca tggaccgatt atccgctgga
    46681 aattgtcgaa attggcgctg gcgaaattct cgatgatggc ctgcgcaaag tgaccgctta
    46741 tccgatggaa cacccactgg aatgttatgg ctatcgtatt gaagaacatg ataaaccggg
    46801 tgcattaaat gcccaggcat taaaagctgc tggcgtgccg cctggcccac tgtttcagga
    46861 attaaaagcg ggcaaaacaa tcacgctgga agatggaagg cagattaacg gcgcagatta
    46921 cttagctgct ccagtgccag gtaaagcgct cgctattttc ggcgataccg gcccctgcga
    46981 tgccgcactt gacctggcta aaggtgtcga tgtcatggtg cacgaagcga cgctggatat
    47041 aaccatggaa gccaaagcca atagtcgcgg ccatagctct acacgccagg ctgcgacact
    47101 agcccgtgag gctggagtcg gcaagctaat cattacccac gtcagctcgc gctatgatga
    47161 caaaggttgt caacacctgt tacgtgaatg caggtcaatt ttcccggcga ctgaactggc
    47221 gaatgatttc accgtgttta acgtttaact
//
LOCUS       DAKISA010000022        47129 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.022, whole genome shotgun sequence.
ACCESSION   DAKISA010000022 DAKISA010000000
VERSION     DAKISA010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47129
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.022"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            241..969
                     /gene="mpaA"
                     /locus_tag="OVW45_001263"
     CDS             241..969
                     /gene="mpaA"
                     /locus_tag="OVW45_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCV5525766.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(944..1909)
                     /gene="ycjG"
                     /locus_tag="OVW45_001264"
     CDS             complement(944..1909)
                     /gene="ycjG"
                     /locus_tag="OVW45_001264"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCV5525767.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKIGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            2028..2534
                     /gene="tpx"
                     /locus_tag="OVW45_001265"
     CDS             2028..2534
                     /gene="tpx"
                     /locus_tag="OVW45_001265"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCV5525768.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTDVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(2578..4119)
                     /gene="tyrR"
                     /locus_tag="OVW45_001266"
     CDS             complement(2578..4119)
                     /gene="tyrR"
                     /locus_tag="OVW45_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCV5525769.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICVTQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVQRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(4267..5328)
                     /locus_tag="OVW45_001267"
     CDS             complement(4267..5328)
                     /locus_tag="OVW45_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCV5525770.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWQCLWCLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(5325..6722)
                     /locus_tag="OVW45_001268"
     CDS             complement(5325..6722)
                     /locus_tag="OVW45_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCV5525771.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            6877..7875
                     /locus_tag="OVW45_001269"
     CDS             6877..7875
                     /locus_tag="OVW45_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCV5525772.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHVITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(7986..8639)
                     /gene="ompG"
                     /locus_tag="OVW45_001270"
                     /pseudo
     CDS             complement(7986..8639)
                     /gene="ompG"
                     /locus_tag="OVW45_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(8995..9606)
                     /locus_tag="OVW45_001271"
     CDS             complement(8995..9606)
                     /locus_tag="OVW45_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCV5525773.1"
                     /translation="MDKAEKTVSSAERFIDMEGINRFSERLAMAMGNMTNVELATLCG
                     LSEATVRNYRKGKNFPPLDKLALIAKACNCSLEWLATGKNEDECGKIEKNCDSGSGID
                     QALSSLSPQEKQLILTFIRREGTNNLVRLAAMNTSTISQDAVESIIDALPLRPVLKNA
                     IKIGMAGSEETDKEILRVIEKYNSSNNATQESGAVTVKKNVVG"
     gene            9763..10029
                     /locus_tag="OVW45_001272"
     CDS             9763..10029
                     /locus_tag="OVW45_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCV5525774.1"
                     /translation="MRVSRQASNNLFVREGCDWSAKAVYAALENKGTDLPELERELGL
                     KSGSMRNVFYRQCRKYEAAIAEKIGVEPAVIWPSRYQVDAREVA"
     gene            10040..12130
                     /locus_tag="OVW45_001273"
     CDS             10040..12130
                     /locus_tag="OVW45_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCV5525775.1"
                     /translation="MWVTAKECVGLPDLPMMEHNIRHRLTKAAAGNAAMLRKRKGTKA
                     IEFNIDCLPSGAQQVLRERYALQLMTQKADESPAPVVTKARRSSAVVDAVEAYRGSPQ
                     LMLERLNALTENQRQVADARIAIVSEVLKVAQQPGFSCAKAIRFIVDNLARSQLDERI
                     VAMVETANAKKGNSRALSEITLKRWIAAFNKAQNAAERLLLLAPGKRQEIKAEDINWL
                     PEFLAQYRQSNGRPMTEAYEDFVAEWQYRHADEPYMLDIMPSYDTIRRAMKKLPEVVK
                     QKGRVTGSEYRQLEGFTRRDWSRMPVNYVWIGDGHGMKLKCAHPVHGRPFAPEVTFVI
                     DGGTRFVVGWSLDLAENVFAVAGAIQHGIRHHGKPFLYYSDNGSGETADILDKEVVGI
                     LPRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARKYRTYFGKGADRETLRKTNRDLR
                     SAFTALQQGKRLNARQQSAMRDLPSWSELIDAIRDGVEWYNNRPHDELPVKPNGKHYS
                     PAEFRKKRLAEEDTEIEWLSDVELRDMFRPMVERPVRRCEIRWLNNIYYAPELRDEHG
                     RKVLISYDIHDAERITVRRLDGSVICEAVWDDNKREAFPVSAEYHKQQQRLKGMRKRA
                     EEKIRDAEDEIVNVLEHKPQEPWLENIYRPVGNTVIVQQPVADDEPDEEYERNFQRGL
                     QLLEAKLKENDPLA"
     gene            12206..13138
                     /locus_tag="OVW45_001274"
     CDS             12206..13138
                     /locus_tag="OVW45_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCV5525776.1"
                     /translation="MTDINDVIKTIDELIDGGVLTQYAIAREAGISDGTLSAFRKGKY
                     KGDNAAVAASLRSWYENWNKQSALPEPPQFVETQTVQELRALFQAVRLMGCINVIVGV
                     PGVGKTATARNYCQEQPNTWMITLSPAHSSVTECLLELADALGIDYTRANKGALSRAI
                     RRRLMGTRGLVIVDEADHLGIDGLEQLRAIQDATGIGMVLIGNPRGLFKGGRRAFDDL
                     SRLFSRLARTKQLHKAKKADVLAIAMAWGISGEAELAVMQAIAEKPGALRVLTHTLNQ
                     AWLTASGEGAALTEKHINAAFKEVYTNPELLSQV"
     gene            13141..13362
                     /locus_tag="OVW45_001275"
     CDS             13141..13362
                     /locus_tag="OVW45_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525777.1"
                     /translation="MAVFNIPDIYGRFYLVNFDNVKVISLAENKECGDLLFEFNDRTR
                     MVISAGLDREGATEVYSGICRSVGAKQVS"
     gene            13375..13629
                     /locus_tag="OVW45_001276"
     CDS             13375..13629
                     /locus_tag="OVW45_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525778.1"
                     /translation="MNMQSCGNKMNLFDSLNSARRLTELAGAVLERSKRYPQRFALKT
                     TPPVGNVQGTGEIEITIQTNGLRRRVKATRISGCTVYWEV"
     gene            13631..13912
                     /locus_tag="OVW45_001277"
     CDS             13631..13912
                     /locus_tag="OVW45_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor protein"
                     /protein_id="HCV5525779.1"
                     /translation="MKKNLIAWAWSSGLIEFGYVLPEGALPIVAGKPATVRHVIEVMA
                     RHGRDEQEQLLVPGIPEAVTEEEAFNAMIRFCREVRRRVSYPNRTRTRG"
     gene            13909..14181
                     /locus_tag="OVW45_001278"
     CDS             13909..14181
                     /locus_tag="OVW45_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525780.1"
                     /translation="MSKVVRIIFEYKEDVICKNSDGSTHRGVSLDIRSTGIKKKDNGP
                     AMIFGVVMLAESRNFAEFVAMKASAFMKDRGMASGVINGNEFNQQG"
     gene            14186..14479
                     /locus_tag="OVW45_001279"
     CDS             14186..14479
                     /locus_tag="OVW45_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525781.1"
                     /translation="MSKVRVIFEFNHVSHDEKLAGNDCVEVHEKIGVDVKTERDTDNR
                     PTSLCDVYASILQYHSPAIIQFLSAEFQASAQSFGADAIIKRHRVHKASGTLQ"
     gene            14491..15021
                     /locus_tag="OVW45_001280"
     CDS             14491..15021
                     /locus_tag="OVW45_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HCV5525782.1"
                     /translation="MAKRVTKLKAAAEAAPQTREEVSRDIRTLGDIQREALRLETAMN
                     DEVAEITARYTPQIENLKKQIKVLFKGIQDWCKTNRDELTNGGKTKTANLTTGTVSWR
                     LGTPSCSVSRDVEGVIEMLRRMGLERFIRTKEEVNKEAVLAEPDAVKGIAGIKVNKGA
                     ESFYVEPFEQDAGLNK"
     gene            15167..15700
                     /locus_tag="OVW45_001281"
     CDS             15167..15700
                     /locus_tag="OVW45_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525783.1"
                     /translation="MIDAKVLEGVKNWLCIYGRLTCGVLAEKMNMPPSSMVYFLRDAV
                     DAGVLTECNGFYDIPRPRPVQPVRRKCSQESAADDVQWCGFRKSLPWIEGHDIPSMAW
                     EFAQGVLTCETVYVVAEVDEQAMKEGVPQFVMAYIDIRLGVIICGLSGWNITEHVLRY
                     LIVDRTAAPAGISAEVA"
     gene            15700..16215
                     /locus_tag="OVW45_001282"
     CDS             15700..16215
                     /locus_tag="OVW45_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525784.1"
                     /translation="MFFKTSNPSALAAWQKYQQDCQKVKDEAKRLEAVLNVACRSVFV
                     SGISGFCFKGLRFTEDKYPFHRDLWRKPTASNGWSCTPRTSRIPKTLRVASDELNSLW
                     REYSPVTYARTDALLFWLGIDFSAILFGPVKWFCVDDVIYLQCGVKPAKQKMTEILSD
                     EFYAAEKRVRG"
     gene            16219..16770
                     /locus_tag="OVW45_001283"
     CDS             16219..16770
                     /locus_tag="OVW45_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SANT/Myb domain-containing protein"
                     /protein_id="HCV5525785.1"
                     /translation="MMILQPMGRKGRAPAHVRPWTPEEDALLIALYSSTPVKDIAARI
                     KRTVWAVYNRTGVLRSSYPELLKYKHPRFTPDEDKFIRKNARTMTCQQMGEYLGRNKD
                     SVRCRAGMIGAGLTKCGELRPGTRISDDDVRLIRALRDSDYPRRLSFREIGEKFGISE
                     HSAHAVYYRRRTAEDAVLRELTP"
     gene            16767..17096
                     /locus_tag="OVW45_001284"
     CDS             16767..17096
                     /locus_tag="OVW45_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525786.1"
                     /translation="MITTLFVESDEPLVCAAGMPLCGGTLTGVYFGDLRGYPWHSLND
                     AFPPDMEAVVLIVQYGHRQELRIGHMGYEGFFVDEETGACLEDEDGQVTHWCHISALP
                     ELREVNA"
     gene            17093..17443
                     /locus_tag="OVW45_001285"
     CDS             17093..17443
                     /locus_tag="OVW45_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525787.1"
                     /translation="MRSYIDNEKLETISDCLSLLAKIKETIEEIKFQLEYAPCGDDAW
                     RNAARKALAVFQKQRRAVEYRLAVLRQEEKERNIRRHERVNDFLVRELKGRVPESVFF
                     ECEAIARSKALETD"
     gene            17459..17767
                     /locus_tag="OVW45_001286"
     CDS             17459..17767
                     /locus_tag="OVW45_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525788.1"
                     /translation="MSIELRSSYEYRKILIAGGMKPEDAEKFVSFMDKECDKRDMPEI
                     IMDDMILDSAVALSPLWIVHTLAEIAKRSDKQAAVAALRLLAELSCLVNPNTFFDEPG
                     "
     gene            complement(17729..18139)
                     /locus_tag="OVW45_001287"
     CDS             complement(17729..18139)
                     /locus_tag="OVW45_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2513 domain-containing protein"
                     /protein_id="HCV5525789.1"
                     /translation="MKIDYDEVLSILTTFQDAETPFLTLQDLGMAEAEGEEKDKKVFH
                     LMLLADNGSIVNGDMRSETPKYIGFFFHSLGVGFRNTPIMLTQQGHDLANALRKKPIL
                     ERIKKEFTDAPFDLIKEVTKSMLTRFVKERIGID"
     gene            18204..18716
                     /locus_tag="OVW45_001288"
     CDS             18204..18716
                     /locus_tag="OVW45_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HCV5525790.1"
                     /translation="MNRASLITLIHVAKCDLQLDHETYTSVLLAATGKPSCRDMSPDE
                     LSRVLDVFKKRGFKVRQKPVNRALKPGTVTAKIRAIWKVMHRQGFISDGGETALNRWV
                     KSQTAAQNGGEGVANWQWLEQHPALASDVLERLKRWHRRKMLDVLGMSARTVMSYERV
                     CRLYEESSPF"
     gene            18787..19209
                     /locus_tag="OVW45_001289"
     CDS             18787..19209
                     /locus_tag="OVW45_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCV5525791.1"
                     /translation="MAETQMSMFGDSEQLHALIDRLDDIPDDELKKNWPGTLRDLVDV
                     ISAELHRQGIEPAQAGMLARKVAAAQAGYMGGRGYYLPVGESLFTELRNNEIFSRWSQ
                     GEKIETLRRHYQMSETQIYSVIRNQRRLHQERTQPRLF"
     gene            19281..19781
                     /locus_tag="OVW45_001290"
     CDS             19281..19781
                     /locus_tag="OVW45_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCV5525792.1"
                     /translation="MPKLPAPLRKKLIALVLAGAGTFTIATHYTGYWEGKENTTYIDP
                     TGTPTICYGHTGPDVKPGMTKTDEECLELLEKDMKWAFAAIDRHVQVPLTRGQTVALA
                     SWIFWAGETNFRNSTLLRLINAGQMPGSCKQYIRWIYSKGVKLPGLEARRSADEWLCR
                     YDLPKV"
     gene            19816..20244
                     /locus_tag="OVW45_001291"
     CDS             19816..20244
                     /locus_tag="OVW45_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001388992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525793.1"
                     /translation="MCRCGAPGCAVAAVNNPLPALIAGGCMAALGVMSVLAAVVWGMH
                     QKTQRLEDNNHVLVRERDEARLVLANQQRTLQLISQISKAATNEKQQNMQHSEEQQSI
                     VRRSLATVPAASVPVPDDVADRVRRAVCEIRAGEAGADPR"
     gene            20228..20446
                     /locus_tag="OVW45_001292"
     CDS             20228..20446
                     /locus_tag="OVW45_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525794.1"
                     /translation="MPIPAEWLADCLVPPVPQPFTFGASVTYNLQLLAVIKNCNVDKA
                     SIRRLEAQRQHEFTDMAGTTAVPAGKTK"
     gene            20457..20684
                     /locus_tag="OVW45_001293"
     CDS             20457..20684
                     /locus_tag="OVW45_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HCV5525795.1"
                     /translation="MDDSDCAQAVVERATERALCDRLTRKCRVEETPGRRVCADCGGD
                     IPAARLVAVPDAIRCVNCQSIMEAGHVGQHR"
     gene            20665..20976
                     /locus_tag="OVW45_001294"
     CDS             20665..20976
                     /locus_tag="OVW45_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 family protein"
                     /protein_id="HCV5525796.1"
                     /translation="MWVSIVKDYVVPILSATATAGGIFMALMRKTFVPREAFEKLSDR
                     VERVESRISGLPTQQEVNRLNIEITTLRGELKATNATLKSVSHQNELLLEQAVRKKHN
                     E"
     gene            20969..21259
                     /locus_tag="OVW45_001295"
     CDS             20969..21259
                     /locus_tag="OVW45_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCV5525797.1"
                     /translation="MSDFITEDQRLVILRSLADYNGELGESVLQDCLDDYGHRVSRDT
                     VHTHIAWLAEQGLVRKRTLVNGYFIAELTGRGQDVAEGRATVPGVKKPRARG"
     gene            21262..21843
                     /locus_tag="OVW45_001296"
     CDS             21262..21843
                     /locus_tag="OVW45_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3486 family protein"
                     /protein_id="HCV5525798.1"
                     /translation="MDKPTRGRVRKVDLLPDSIRKPLLEMLREKRLTQVQIREEINRL
                     IREAGLPEEQQLSPAAISREASRNELIARNLRDLREQTKAMMAELGDKPTGETTALIL
                     EMSRALMYRRLRAATESLNSDSDVDMRLIKDILLSAQRAESAAERSIKREKEIRAAFA
                     EEMANAVTDELRGVDGMSEQIEFRIRQMLLGKA"
     gene            21854..22102
                     /locus_tag="OVW45_001297"
     CDS             21854..22102
                     /locus_tag="OVW45_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525799.1"
                     /translation="MATDNRSLMSSAHLKAFSLATQLPPETIKACRELTELSIAGEIS
                     LEQLHSLLAEAATDELSGVDVMTEELESRIKSILTGKA"
     gene            22102..23766
                     /locus_tag="OVW45_001298"
     CDS             22102..23766
                     /locus_tag="OVW45_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525800.1"
                     /translation="MNKAKEKSPSLIQMTPPRKIDLSGEKALLGVDVPDSLDLPGDMP
                     VFLDYQARWFADESQVCIAEKSRRTGLTWAEAGRNVITAAKPKRRGGRNVFYVGSKQE
                     MALEYISACALFSRAFNQLADADVYEQTFWDRDKKEEILTYMIRFPNSGFKIQALSSR
                     PSNLRGLQGDVVIDEAAFHESLDELLKAAMALTMWGARVRIISTHNGVDNLFNQYIQE
                     AREGRKDYSVHRITLDDAIADGLYRRICYVTDQEWSPESEQKWRDDLYKNAPTREDAD
                     EEYGCIPKKSGGAYIPHALIEMAMIRDIPILTFEAPDNFISRAAWLRESEVLTWCEEH
                     LKPLLEKLNPRSRFSFGEDFARTGDLSCFVLLEITESLAKREVFRVELRNLPYAQQEQ
                     VMMYILTRVPALVGAAFDATGNGGYLAEAALLAFGPDIIDCVMLSPKWYGEWMPKLKA
                     EFEDQNILVARHQTTLDDLRHVKVVNGIPQIDKGRTKDQNATAANARRHGDFAVALCM
                     ANRASYMEGFILDESACQALPERSRAMEGGYRDDDEAYHEFDRGGW"
     gene            23766..25355
                     /locus_tag="OVW45_001299"
     CDS             23766..25355
                     /locus_tag="OVW45_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HCV5525801.1"
                     /translation="MGRIIDTDGKPFSFDPEMQSAALDIPQIASRYIEHPASGITPNR
                     AAQCLRGAERGDLIAQSDLAADIEEKDTHLFAELGKRRLAIQGVPWSIEPPPNASANE
                     KKDAEMLDEYLHSADWFDAMLFDATDAILKGYSCMEIEHGMLGKMHIIRAIRWRDSGH
                     FCLNPDDLSELRLRDGSHSGVAFQPFGWIVHQSRSRTGYGGATGLVRTLIWPFIFKNY
                     SVRDLAEFLEVYGLPMKVGKYPSGATPEQKSALMRAVMDIGRRTGGIIPAGMSLEFQA
                     AANGQADPFETMISWGERSISKAILGGTLTTEAGDKGARSLGEVHNEVRREIRDSDLR
                     QLAATLNRDLVYPLYALNTTHTIDIRRLPRICFQTKEPGDITKITSAVMQLSTGMDIS
                     DPWIREQTGIPQPTPGEAIFRVRQSGNEPAQTDRETPPEKQEKTEQTALSARLPEAKS
                     TPRDELDDMGDAVPARRLQEAIDPVLEPVIDAIRTRGLADALADLPALYREMDDSRLM
                     TLLSDAMFAAEMKGMLDGSGD"
     gene            25339..26670
                     /locus_tag="OVW45_001300"
     CDS             25339..26670
                     /locus_tag="OVW45_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HCV5525802.1"
                     /translation="MAQEIDLGYAATLPSKETVAYFRAKGAHISWNWFETAADVHARS
                     FTAAKAARLDVVTTLQAEVQRAIDEGISQKAFIRTLTPRLQKLGWWGKQIVVDSDGNA
                     EEVQLGSPRRLALIYNVNTRVAYNAGRYTQMMNNTDTHPFWQYVAVMDSRTRPSHSAL
                     NGLVFRYDDPFWKTHYPPNGWNCRCRVRPLSQARLDAMGLSVSSGEDHLSTRNVEAGV
                     DKQTGEVREMPVTTYSDGTRTMTPDVGWSYNPGSAAFGTDQALIRKLIEVKSPALREM
                     VVQEMNNSPERQLAFRIWAKNIMQTRRGGNDIRTLGFMTESIAQAVESRTGTPPARLL
                     AMSGKNVLHADSVKHQNDGIALMPEDFGRLPAMLAKPKAVLWDKRHKNLMYIVENKDG
                     SVQIAVNAPYSLKRQPDKLDVIVNAYRVINMDKLKSDIRGGMLEVLEGQID"
     gene            complement(26672..26751)
                     /locus_tag="OVW45_001301"
     tRNA            complement(26672..26751)
                     /locus_tag="OVW45_001301"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            26792..27265
                     /locus_tag="OVW45_001302"
     CDS             26792..27265
                     /locus_tag="OVW45_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HCV5525803.1"
                     /translation="MSSIDAAVVVDVTRLQRVFARLQFVGGGKDLARSVAESLLSSSE
                     MAFEQEKEPDGERWHDWSDPYRKWRTRKGYTPGKILTLNGDLARRLTTDYGDTWALIG
                     SNEPYAAIHQWGGLPGMPPGPAAIGARPYMGFDQVAEQEIMDEIRKRFKKATETP"
     gene            27443..28567
                     /locus_tag="OVW45_001303"
     CDS             27443..28567
                     /locus_tag="OVW45_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HCV5525804.1"
                     /translation="MKTKNTPKLAYAILNAISLSADGDGDWCQIMPAGRVKARDGRPE
                     KPAEGWLINHAAVERMVSRVVALNQPVKIDYNHQTLIKGHPAPAAGFVMASPENFRFS
                     EERGFEVRPDWNPPAIEHLRNNEFPWFSPVIGYDEITGEPVELRMLAITGDPGLTGMN
                     PVAALSADDLYNALNPPLKDTSMNEQLRQLLTALGLTVADGDEFTPELGTAALSALTG
                     IKTRADAHDTLKTQVASLSAELETAKGTPTGGTIDLTKYVPVETYNALRTEYVALSAQ
                     HGSTTLEQLLDKAESEGRIFKSERGYMEGLGQQIGVAALSAQLDARQPVAALTTLQTD
                     TVTVPDKKTATAALSAEDIAAAHLLGKTEAEFLKMKEEMQ"
     gene            28567..29514
                     /locus_tag="OVW45_001304"
     CDS             28567..29514
                     /locus_tag="OVW45_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HCV5525805.1"
                     /translation="MPTPITPAMITALMTGYRSDFQAGMSMAPSQYQKIAMTVPSTSK
                     SNTYGWLGQFPQFREWIGSRVIEKMKAYGYAIVNKTFEGTVAISRDDFEDDNLGIYSP
                     LFQEMGRAAAAQPDELVFAALRDGLNAACYDGQNFFDTEHPVYPKVDGSGDAQMVSNM
                     FVAKTGSVGAQADYSGPAWYLLDCSRAVKPLIYQDRRKAELVAQTKVDEGRAFTDNEF
                     VFGASARRNVGYGFWQMAYMMQSPLTLDALWHGWSAMRDFKADGGRKLGIKPTHIVVP
                     TSLEKQAVQLLERELFADGNTTVSNEMKGKLELVVADYL"
     gene            29558..29911
                     /locus_tag="OVW45_001305"
     CDS             29558..29911
                     /locus_tag="OVW45_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525806.1"
                     /translation="MNEHHTVAATDDSGLQVSGDSPVTVLAEVRCSRAQFRRAGFLFM
                     RGRQQVDVTPEQLARLEAEPCLTVRILQTPGDDAGAVAGVVHAETGADLAEAGQDAPR
                     KKTGNNARQAKKARA"
     gene            29908..30327
                     /locus_tag="OVW45_001306"
     CDS             29908..30327
                     /locus_tag="OVW45_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp436 family protein"
                     /protein_id="HCV5525807.1"
                     /translation="MNYATETDMRARYREDLLRPLLAVPRSDEPDTRKLNRALTDASA
                     LIDSYLSARYTLPLAVVPAVLVQHCCTIAFYYLCDQRASDQARDRYREALAWLKDVMN
                     GNVPVGVDTDGAAPESGDLPQVQSDAAVFGRNQKGFI"
     gene            30324..30887
                     /locus_tag="OVW45_001307"
     CDS             30324..30887
                     /locus_tag="OVW45_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HCV5525808.1"
                     /translation="MITETEQAYIARIREYFGNELVSVDTHPGDWSDSVLRTMLINAP
                     AIYVAWLGAGEGRTRGRLVSHWVFYVIGDMLNGREVSRPGLYQIVARLITVLDGFRAE
                     KTSPLYFEKAVNGYTETQAGSGAVMYALYFSCEEMIDPLTDISSLDDFLRHYETFTEP
                     EGTPEFKAHIRLPGATAAGENAGPSEE"
     gene            30891..31169
                     /locus_tag="OVW45_001308"
     CDS             30891..31169
                     /locus_tag="OVW45_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCV5525809.1"
                     /translation="MTTIFIKPAPGCLIRDPDTMTPLSQDGEEKPFTPFWCRRLNDGD
                     VIVVDKTEEPAPAATTGNTGAAAKPAGAVAEKTAAVAEKNAASDKGTS"
     gene            31169..32671
                     /locus_tag="OVW45_001309"
     CDS             31169..32671
                     /locus_tag="OVW45_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCV5525810.1"
                     /translation="MINFDYIGDNNRIPLVQIEINNSMAVTGTPPQRQAVLLFGQAAM
                     KDTTIQGRGQLDVPVRITRASQARELWGRGSMIALMVEEFIAINPDTELYAIAQGAGT
                     GQSRACVMNIIGTALADGVLSVYIGGRRYLLPVSKGKKGKELVEELAKVINADTDAPF
                     TAIAAEVSGDNAEGLKGSMGVNARFIGECSAHDLRVNYYDGETTPAGLTLELVAPTQK
                     ATNPDITRSVSGMGNRQYNYIVMPYKDAANLKILADELLKRWGPVKMSDGMVWMAHTG
                     TFGTATSFGESRNDFLFTCTSIPKAPEPDYVWAASICAVCAPSLSTDPARPLQTLALP
                     ARMAPEAPARLTREERNSLLYTGMSTVTVAAADMVQIERQVTMYRQNAYGESDPSYLD
                     VETIYTLSYLRYSLRTFITQRFPRHKLADDGTPVRAGQNIVTPEIMKLQLISLGEEWV
                     KLGLVENLDTFKKNLLVERNTKDRNRLDVMCTPDLVNQFRFMAAQIRFIL"
     gene            32680..33045
                     /locus_tag="OVW45_001310"
     CDS             32680..33045
                     /locus_tag="OVW45_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCV5525811.1"
                     /translation="MSGKQYQGTATIRVNGQEYATLEGATFSPSGFEREVVKGAKVYG
                     YREKPREATMDCKFPAGGEGSPAADEINTWTAVTIEFVADTGEVHMMTKAWSSEPASL
                     DGGGDISAKFASATSTRVQ"
     gene            33060..33536
                     /locus_tag="OVW45_001311"
     CDS             33060..33536
                     /locus_tag="OVW45_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCV5525812.1"
                     /translation="MSTRKKKTAVSDEAVMEAIRDALEDGDPRTAGLAEQLANGYVDL
                     LDGLPFGESREYRVTFRELTAKDSIDAESEAERLMETQNGPVLVASPALRGIALLRRQ
                     IAAVGQIEGPLSPRQIGQLSERDLSRLMAAVSLLDSAMAGKLVVERGRTDAVPGSD"
     gene            33664..35721
                     /locus_tag="OVW45_001312"
     CDS             33664..35721
                     /locus_tag="OVW45_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCV5525813.1"
                     /translation="MAKNLKASLIVDLLGNISAKSRQWSQELGAFSRSGRTGLGGLGN
                     AARRAGQETELVGSRMQRTLAGVRGSIRTVTSDFDRLQGSITGTIGRISNLYGMLAGG
                     AAVYGFNKAFIRPAAEMENYILRLNAINHGDTAKTEAVKAWAVQNAKDTTWGLAGVMQ
                     EYASSRGFGMSDREARRFITMLQDQGGYHGWSLSDAQGASLQLKQMFARQSIQAADAN
                     ILTGYGINVYQLLADKLGVNQKIIREKGEKGKLGPDSIRLLFQVMAEQAKGAQKNAMN
                     SWTGMTSMMGDVWDGFAREVMAKGPFDSLKKSLKGFLDYADAAQKSGLQDKLATQTAS
                     ALNQGFEYARDAATGFYRAIQKVRETLQALRDAGYGDALDRIGQGAQTAAKYLMYMYL
                     ASRALKVLRFAGTGALRLGATPLRYGMAMTSVLTSPFRKPQTTVPGTQPGRAGRFLNF
                     LTGVNPAAVQPVLVTNWPAGGLASGGGDVVVSGDGKTVRGRKKRGPGRGRGVTTVVTA
                     GEQLAESAGKQGFFGRMMSRAGGLLTAAGNRMGLGRFAGLFRGAGRLGGGALWAGAMA
                     APVLLDSSASAADKAGAVGSLAGSIAGGALGAAAGPVGVAIGSTVGSYLGDYLGGWLT
                     QAWQKLRGSSDENGGQATAKTAARVELVAPEGWRARSIDVDDTAQHGLDVNVWNGGNY
                     GLY"
     gene            35708..37066
                     /locus_tag="OVW45_001313"
     CDS             35708..37066
                     /locus_tag="OVW45_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HCV5525814.1"
                     /translation="MASTDTPGRGSFRGVPFLVYQEQRERGGRNIVRREYPLRESGGA
                     DDLGPKLPEFTFTVLVTGDDLQTQRSRLRDALRAPGAGELMHPDYGTLNVLINSFESR
                     YNASEQGTVEFTINVIPASDDTAPAVAEDTAAALEQKSGSAMNQLFNTLSDGWTVISD
                     GLHDVQAMTDTISDKIDALENAVSGMGIVQDISAFTASFTALKGNAAALINAPRRMAE
                     SLAGMFAVLTGLPGDPSLSLTGKAGTPSGSLTTNRDSIAEQAMPQLYRTLSSLRYTLS
                     EQDDPQRLIGLTPAAQKNIRLLRSVMQSATLVSQAQTLGKLLDQVIRQNTTHVQDTDR
                     SGGLAWLESSADVQRINRDLSDAMEQQVLDLSAQGYTGTALALRDAGLALTEDLTTRS
                     VHLPGAFQVMVRTTEPALVTLYRATGNSSRWQYFVRRNNIPDPVFVPGGRSVEVISEQ
                     QD"
     gene            37050..38174
                     /locus_tag="OVW45_001314"
     CDS             37050..38174
                     /locus_tag="OVW45_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCV5525815.1"
                     /translation="MSSRIELYIGGSIFSGWLTVSVRRSLEHLAGSFELGLMLPGERI
                     PSALRTGQSLTLRINGQTVISGWLDQVSQRISATRHQISISGRDKTGDLVDCAAIHPG
                     SQWRNRTLAQIAADLCAPFGIAVRWQVNDDTAARPFSSFTLENSETVADALTRAARHR
                     GVLVTSNADGDLVFTQAGSQQTDRLVLGDNLLDADYNTDWRGRYSEYRVRGHGRGGGK
                     RGDSESAARLAAPVGVISDEQIGRYRPKIILADQQTDTTGARQRALREMRRAIARSER
                     FSATVRGWFRDDGRLWDVNLLTGVSALRFGIEQTELLVCQVEFLLDEHNGEVTRLVLA
                     PRDGFIVPAEPDSKGRGGSGDDVDAFIRQQMKKQGISFDE"
     gene            38164..38778
                     /locus_tag="OVW45_001315"
     CDS             38164..38778
                     /locus_tag="OVW45_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HCV5525816.1"
                     /translation="MMNDEVFSRLIAPVTRGIRLLFGRGVLTGTHDELKMQNVQLTGM
                     DGETFDDVERPQQYGQISVPLPGAETFFACLGGQRDQTVVLVVEDRRSRPTGLTAGDT
                     GVYHHEGHRIRLTKDGRIIVTCKMLEIYADEGMRVDTPEATFTGNVTVDKNLHVKGNF
                     VLDGTGKSDGLFTMSDAVIAGITYSGHVHQDNGKGSKTGAPENG"
     gene            38771..39208
                     /locus_tag="OVW45_001316"
     CDS             38771..39208
                     /locus_tag="OVW45_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HCV5525817.1"
                     /translation="MADIAIVWDQGCGSLQLNGADLLTDDSLLTAVLISLFTDRRALA
                     SDEIPDGTRDRRGWWGDSFRPRPIGSRLWLLRREKTLASVISRARAYADEALGWLSQD
                     GVASSVVCHAERVGHARLALSVRITLPDGSVRPMIFYADLKGE"
     gene            39208..40290
                     /locus_tag="OVW45_001317"
     CDS             39208..40290
                     /locus_tag="OVW45_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCV5525818.1"
                     /translation="MPYQPLPLAQLITQTQQDISQRLPGSQPGVNETTLNAIAYAQAG
                     LSAQEHEHLAWIARQIIPTEADEAELLKHCAFWGVMRKPASRGDGPVQLMLTTDAGIK
                     EGVLLQRSDGVVYRITTSLTGKAGTLNVSVEAESAGRAGNAPAGTKLTFITPQAGINQ
                     TATVTGTGITGGADVETVPELLSRLVFRVQNPPSGGTQYDFERWAREVPGVTRAWCRP
                     EWPQAGSVGVTFVQDNNPDIFPGDGDVQRVADYIRSHDDPATGQPVGQPLGPTVTVFK
                     LTNKPVPFSIRIIPKTPENQAAVKQALTDLLYNESRPGGLVLPSSFWRAVAGVKNLED
                     FEVRSPLTSVQAGDSELLTVGEITWL"
     gene            40281..40841
                     /locus_tag="OVW45_001318"
     CDS             40281..40841
                     /locus_tag="OVW45_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HCV5525819.1"
                     /translation="MAVTLTPHQRALLQLLPDGLAWDKRPSSVLASLCLGLSHSTARV
                     SWTGKQLLAERFPDTSRLLLEDWERYLGLPECDMAGATLTERQRYAGNKYRMKPSLNR
                     EFYIRFAAEFGYQIDIQPSPDSLWVSIVTINSETGYRNMNVLDDILTPLRIYEGGALE
                     CILNRYKPAWQTFIYVYANSHEEETI"
     gene            40841..41752
                     /locus_tag="OVW45_001319"
     CDS             40841..41752
                     /locus_tag="OVW45_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCV5525820.1"
                     /translation="MFHVDNNSGVANMPALAPAQSNTTTWFTEGDGQKGISWIGQDWL
                     NILQAELLNILAEASIQPDKAQLNQLTLSIKAIIAANAFSRKNNLKEIADAGAEAQRL
                     ARGYLGLGTLATKNSLGPGDVNALAKDQNLADLENKGTARNNLDVYSKSEGDNRYLRR
                     EQNGADIPDKGAFIDNVGLRETVNKAANALPSDGTAVAANKLSTPRNINGVPFDGTQD
                     IYITSGMTEATADNKYVSNVQLGAQSYHSPGGNEMSWSYGAPSGCMLSGINVQDTGKS
                     SADNIGGVYYRPVQIYIGNAWRTVSSV"
     gene            complement(41787..42308)
                     /locus_tag="OVW45_001320"
     CDS             complement(41787..42308)
                     /locus_tag="OVW45_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCV5525821.1"
                     /translation="MQLINVKRYYPEQKPFGNDVQYFQSEDGKDFYESLPLFTKKYKL
                     CITPDSGVICSISQDASALYPAGFSVVETDELPEGTDISGNWKFDNGVISQLPVNYTK
                     KAEALRQSYLNQAYEKINDWRTELQLGTISDENRAALTRWMKYISQVKKMELPAIKNK
                     AEFDAIKWPEKPE"
     gene            42613..44667
                     /locus_tag="OVW45_001321"
     CDS             42613..44667
                     /locus_tag="OVW45_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCV5525822.1"
                     /translation="MSDSKWKMLSAMPGEFSVKVAGGTVAFIESPFRPSGNKGGITFA
                     DCVIRFSTKEPLWVMPVSGNPSAEITSSGVTGIIPITSDVAGTLTPSDWNAPDNAGPS
                     GSTNGSSSEPEYYFVVILAGQSNGMAYGEGLPLPDSFDRPDPRIKQLARRSTVTPNGT
                     SCTYNDIIPADHCLHDVQDMSGINHPKADLAKGQYGTVSQGLHIAKKLLPYIPQNAGI
                     LLVPCCRGGSAFTTGADGSFSEASGASADSSRWGAGKPLYQDLVSRTKVALAKNPKNK
                     LLAVVWMQGEADLASGSQQHNSLFTAMVQQFRTDLAPLAAQCVSGNATTVPWICGDTT
                     YYWKNAGTDKYEAVYGGYKGKEAQKIFFVPFLTDENGQSTPTNAPAEDPDIVAVGYYG
                     AASRTQGSFVSTQRDSHFSSWARRGVISDRLASAIMLHAGRTAELMRGQTVTPPDEKP
                     SPDTPSKPSTPPADTTTMSTLFAYRASESEGRLTPQGWAAGGGKAQIVDDAGASGGKA
                     MKLTKETGRASWYLEHDAGNGADLLGKGGLVSCRFKLDGALTANRYALALYWPVSSLP
                     QGVMLEGNSGHNLLASFYVQSDATDLNVMYHKGNTAQNTKLGSFGAFNNEWHTLGFRF
                     AGNNSIEVTPVIDGKDGTPFMLSQSPVGTFTADKLRVTDITKNATYPVLIDSIVVEVN
                     NA"
     gene            44812..44994
                     /locus_tag="OVW45_001322"
     CDS             44812..44994
                     /locus_tag="OVW45_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HCV5525823.1"
                     /translation="MTFLQLILLYFCTAVCALYLVSGGYKAIRNYVRRKIEDAAAEKL
                     SKTAQVTPLPKDPTQP"
     gene            45030..45284
                     /locus_tag="OVW45_001323"
     CDS             45030..45284
                     /locus_tag="OVW45_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HCV5525824.1"
                     /translation="MPEIKGTVTEDLVKQALYSGEVNKLLKAQVRKDFEAQIDTYVDE
                     VLAKLIGPAVAASDADNEPKTDAQPEQPDPAQPGTDSTMM"
     gene            complement(45452..45712)
                     /locus_tag="OVW45_001324"
     CDS             complement(45452..45712)
                     /locus_tag="OVW45_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525825.1"
                     /translation="MIENAEIYNDYFSDYSDACKYSSSAGEFICFNFFIKSYVFPPQQ
                     EDGGVPPPEITLHRIASVSMMEQQANELYSILGTILQKRESD"
     gene            46002..46202
                     /locus_tag="OVW45_001325"
     CDS             46002..46202
                     /locus_tag="OVW45_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCV5525826.1"
                     /translation="MYRNIRCRHCNKLLARASFSYLEVKCPRCKTLNQITSQSATEHP
                     TYTRKSCRGEQKASYIPYHQHA"
     gene            46156..46893
                     /locus_tag="OVW45_001326"
     CDS             46156..46893
                     /locus_tag="OVW45_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HCV5525827.1"
                     /translation="MGNRKQVTSRIISTPELIHYNDNIVGYGSRELRVETISCWLARL
                     VVVNKHYSHRFVNNSYLHLGIFSERELVGVMQWGYALNPNNGARVVTGTQNREYMELN
                     RLWLHDCMPRNSESRAISYALKLIRQLYPQVQWVQSFADERCGCLGVVYQASNFDYVG
                     SHETTFYELDGEWYHEICKNAIKRGGQRGEHLRANIDRASVHKFRQFRYIRFLDKRAR
                     KRLNTKLFKVQPYPKPQTVKTGLKESE"
     gene            complement(47024..47129)
                     /locus_tag="OVW45_001327"
                     /pseudo
     CDS             complement(47024..47129)
                     /locus_tag="OVW45_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpG family monomeric porin"
BASE COUNT        11443 a        12000 c        13034 g        10652 t
ORIGIN      
        1 gatcaatccg atgcgattaa catgtgtaga accagtaaga ataaaatacg ccatgtatat
       61 aacattacga cttacatcta agtcagcata attctaatat ctgcacatca ataaaaatgc
      121 aaacaacttc cggcaaaaaa agtgatacaa gatcgatgac atagcgttga agaaaaggat
      181 ttgataatcc gcaaaatttg cacaacactg cctgtaatac ttcctgaacc agagacgatc
      241 atgaccgtaa cccgcccacg cgccgaacgc ggcgcatttc cgcccggaac agaacattac
      301 ggacgttcat tattgggcgc gccgttaatc tggtttcctg cccctgcagc cagccgcgaa
      361 agtggtttga ttctggctgg cactcacggt gatgaaaact cttcggtcgt cacgctctcc
      421 tgtgctctgc ggacgttgac accttctttg cgccgccatc atgtggtgct gtgtgtgaat
      481 cctgacggct gccagctggg gttacgggcc aatgctaatg gtgtggattt aaacagaaac
      541 tttccggcgg cgaactggaa ggaaggtgaa acggtttatc gctggaacag cgccgctgaa
      601 gaacgtgatg tcgtattact gacaggtgac aaacctggct cagaacctga aacccaggcg
      661 ctgtgccagc ttatacaccg cattcaacct gcctgggtgg tctcattcca cgatccactg
      721 gcctgtattg aagatcccag acacagcgaa ttaggcgaat ggctggccca ggcgtttgaa
      781 ttgcctctgg taaccagcgt cggttatgaa acgcctggct ctttcggcag ttggtgtgca
      841 gatctcaact tgcattgcat caccgcagag tttccgccca tctcctccga cgaagccagc
      901 gaaaaatatc tctttgcgat ggcaaacttg ctgcgctggc atcctaaaga tgcaattcgc
      961 ccgtcgtgaa ctgaagcgcc ggttccacat ctaccgccag ccaggtcggt ccgtcaagat
     1021 cggcgaaact gacctgcggc accagcggta aagcggcgct aatggcacga gaggtacaca
     1081 acatgcagcc cagcatcaga ctgaaacctt gtgcacgcgc ttcagtcgcc agcgccagcg
     1141 cttccgtcag accaccgatt ttatcgagct taatgttaac catctcatag cgccctttca
     1201 gcgccttcag attgctacgt gtatgacaac tctcatcagc acaaatcggc aacggatgaa
     1261 taaaattctc cagcgccgca tcgtcctgcg caggaagggg ttgttcaagc atcgcaacgc
     1321 ctaaatccgc caatagctgg caacgcgccg ccaacccttc tgcacgccag gattcatttg
     1381 catcaacgat cagcgtcgca tcgggcacag ctgtgcgaat tgccaccatc cgctcactaa
     1441 taagatggtt atccagcttc actttcagta atttcgcgcc tgcctgccag agtgttgatg
     1501 cactattggc catctgatca ggcgtaccga tgactaccgt ctgtgcagta ataactgtct
     1561 cgggaagcgt tatgccgatc aaatcagcca gcgattgctg ctgttttcgc gcggccagat
     1621 cccacaatgc acaatccagc gcattacgtg ctgcgccggc agggagaatt ttttgcaact
     1681 cctcccgtgt cagccctttc tctaattgcg acacgacgct cataatttgc gccattaccg
     1741 aggcatcact ttctccataa cgcggatacg gcgtgcattc gccggtgcct ttaataccct
     1801 cttcttccag ttcaaccacc accacgcgcg cttcactgcg acttccccgg gcaatcacaa
     1861 acggggtatg taagggccag gcttcctcaa ataccttaac ggttctcatc tttactcctt
     1921 gttgtcgcgg tggtctgcgg attaagcgca aatagcgttt gctgtgttat tgacagttag
     1981 cataaactag gtgtgacgtt aactatatgt aaacaggaaa gataatcatg tcacaaaccg
     2041 ttcatttcca gggcaacccg gttacagtcg ccaattccat cccgcaggcg ggtagcaaag
     2101 cgcagacttt tactctcgtg gcaaaagatc tgtctgacgt caccctcggt cagtttgcgg
     2161 gtaaacgcaa agtgctgaac attttcccga gtattgatac cgatgtttgc gccgcatcag
     2221 tacgcaagtt taaccaactg gcaactgaga tcgacaacac cgttgtgctg tgtatctctg
     2281 ccgatctgcc gttcgcccag tctcgtttct gcggcgcaga aggtctgaac aacgttatca
     2341 ccctctccac tttccgtaac gctgaatttc tgcaagctta cggtgtggca attgctgatg
     2401 gcccactgaa aggtctggca gcgcgtgccg ttgtggttat tgacgaaaat gacaatgtga
     2461 ttttcagcca gctggtggat gaaatcacca ccgagccgga ttacgaagca gctctggctg
     2521 tactgaaagc ataagttaat atgcctgatg gtgttgcacc atcaggcata ttcgcgctta
     2581 ctcttcgttc ttcttctgac tcagaccata ttcccgcaac ttattggcaa tcgcggtatg
     2641 tgaaacgccg agacgttttg ccagtttgcg cgtgctggga taattgcgat aaagctgggt
     2701 taataccgag cgttcaaaac ggctggtgat ttcgtccagc gaaccttcca tcgcatcttc
     2761 gcccacggct accgttgcgg cgtcataatc cggcaacaaa atatcctgtg gacgcagctc
     2821 ataaccgtcc agttgtgtca gtgcgcgata gatagcgttc tttaactgcc gcacatttcc
     2881 cggccacgca taacgcgtaa gtacagtatt caggtcagcg gccagtttcg gacgctgcac
     2941 gccctgctcg tcggcaaagc gggcgacgaa cagctcagtt aacggcatga tgtcctgcgg
     3001 acagtcacgt agcggcggca gattgagcgt caacacgttc agacgataat agagatcttc
     3061 acggaacatg cctttttgca ccagttcgac cagattcttc tgcgtaacgc aaatcacccg
     3121 cacatcgaca tgcacctcat ggtcttcgcc aacccgacgg aaagtgccat cattaaggaa
     3181 acgcagtaat ttcgcctgca tccgtggtga catttcccct atttcatcca acagcaccga
     3241 accaccgttc gcctgctcaa agaatccttt cttcccttcc ggagcatgac caaacagttc
     3301 actctcgacc gcatcttccg gtatagacgc acagttcagc gccaggtaag gtttgcccgc
     3361 tctggggctt gcctgatggc aggcgtaggc aaagagatct ttacctgtac ctgtgtcacc
     3421 cgtaatcagc agcggcgcgc ttagcatcgc cagtttctgc gcctgttcga caacatgctt
     3481 cattttcggg ctgacggcga caatttgact gaaggcgctg acgtcctggg cggcgacatt
     3541 ttgcaactgg cggcccatac gaatcgttga tcgcaacatc accaccgcac cggtcaggac
     3601 gtgttgatca ttttcatcct gaagataaac aggcgtaatc tccatcagga aattctgccc
     3661 attaataacg acatgctcgt tatgcgaatc ttgcggttcg ctttccagcc aacgtaaaaa
     3721 attaaagccg ttaatcaatt gtgcggcggt atggttgcgc aggcgatcca atttttgccc
     3781 aaaaagctga cagctcgccg ggttcgccat atccactttg cttttcatat cgacagagag
     3841 cacaggttca ggcaacgcct ccagtaacgc gctcaacgcc agatgctcac gttcggaagg
     3901 catccacggg acagtacgca catcggtaac acccgcaata cggcgtattt cggccatcag
     3961 actgctgaaa ctctcaaact ccagttcagc aaaattgagg tagattcgcc caatgggatc
     4021 aatctcaata ccgcgtaaat caatgcctct tagcacgagt agatcgagta attcgcgggt
     4081 cagaccgagt cggtcttcac aaaagacttc cagacgcatg ggaaccttca cctgaaaaaa
     4141 gaacaattaa tatgatgata tgacactatt tggatagcag gaaggtttct gtcaacaatc
     4201 attgacacaa agacggaaaa acgggcactt cggcgtaaag atatccggtc agcacccgat
     4261 attgcattat ttttcgctgg gtgttttgcc tttttgcagt gtttctttca cctgaccgat
     4321 aagctgacga cggaaatccc cgaggcgagg tttgtcatcg tcaatccacg gcagcgggcg
     4381 gcaaagctcc atagctttaa tcccgagtcg tgccgtcaga agtcctgcac caatcccctg
     4441 agctgcgcgg gtagacaaac gagcagcgag atcttgcgac atccagtcca tccccacttc
     4501 gcgcaccagt tcgctggctc cggcaaaagc gatattcagc aataccagct taaacagacg
     4561 caaacggctg taatacccca gttcaatgcc atacagcgtg gcgatgcgat taattaaacg
     4621 cagattgcgc caggcgataa acgccatatc gaccaacgcc agcgggctga ccgcaatcat
     4681 caacgttgat tccgccgccg aacggctgat ttcgcgccgc gcctgggcat ctaaaactgg
     4741 ctggaccaaa tgcgcataca ggctgaccac ttcacggtcg ttttgcgttt catggattga
     4801 ggcataccag cgttgcagcg ccggatgcga ctgatcaata cccgcctgct gcgccagttt
     4861 ttcgcaaaat gcgcggcctt tgcccgtgcc gtggctatgc aataaatcac gcgcttcgtc
     4921 gcgttcatgg gcgcgctgtc gcaagcacca taagcactgc cactctgtta ccacagaacc
     4981 tacgccagcg ccgataatca atgccccagc ggcacatcca cccagcgcta cccagtcctg
     5041 ggtttgccag gcattcattg tccactgtac accctgcccg acaacacttg cgccaaacag
     5101 agccagcccg cccatcacca ttttgcgcca caggctgcgt ttcggacgta acgctgcatc
     5161 cattaccgct tcaacttgcc cctcttcttc aggcgcttca tcgagcgtgg ccggggcaaa
     5221 attttgcgcc tgattttcgt caaaggtctg ctgcgccctg aattttggat tctgatcgac
     5281 ctccagcgga ccgtcgaaat caatacgtgg ttttaacggt tcggtcatcg caatttatct
     5341 cctattaaaa attccagcgc ggcatcaaga cgaatatgcg gcagcggttt gtcgacatcc
     5401 atcacctgcg ggcgaaacgc ttcaaactgg aacccttgct tgtcccagaa cgcctgaccg
     5461 ggcaaacgcg caggaacttc accggggtaa acggtgagag gcgcaccatc gctgagccga
     5521 ttaccgcgca atgccggaat tttctcgccg ttgacatcaa taatgccgct ggtggtcgcc
     5581 tgcaccgagg ccagccccag gcaatccata ctaatccctt caaacgccgc attttgccag
     5641 gcatcctgaa tcagttgttg cagtaatgaa accatattgg cgtgctgatc gaccgttaca
     5701 tggtccgctt tcgttgcggc aaacagcagt ttgtcgataa ccggcgagaa taagcggcga
     5761 aagagcgtgc gctgcccgta gtggaaactt tgcatcagct gcgtcagtgc cagacgcata
     5821 tcattaaatg cctgtggccc actgttgaga ggttgcaggc aatccaccag cacaatctgg
     5881 cggtcaaagc gcagaaaatg attcttatag aaccccttca ccaccttctc gcagtaataa
     5941 ttaaaccgct cgcgcagcat tccggcattg gtgtgcttat cggcctgcgc cagtttggac
     6001 tcgccccagg tatcgacatc cggccacggg aagaattgca gcgcgggcgc acctgccata
     6061 tctcctggca agacaaagcg ccctggctga ataaagtgca gcccctgctg tttacagtgg
     6121 tggagataat cggtccacgc ggcggcaatg tctgccagcc ggttttcgtc ggcaggtgct
     6181 agcgggtcca gcccttcgca catcattcgc catttgaccg accattcgcc gcgctgacca
     6241 ttgagtaagc ccgtcatctg gcgtgaccag cttaaatagt cctgcgccag catcggcagg
     6301 tcgagcaacc attcgccggg ataatccaca atctccagat acagcgtgga ggtgtctttg
     6361 aagtggcgta gcagcgaatc gttcgattta aaacgtagcg ccaggcggat ttcactgacg
     6421 ccgcgcgtag gcgtcggcca ggcgggagga tcgccatata actgcgccag tccttcatca
     6481 taggtgaagc gcggaatgcc aaagtcacgc tgaggaatgc gttttacgcc cagcaggcgc
     6541 tcttcacgca ccgcgcttaa cagcggcaag cgtgctccgg cgtgaatatt gagcaactga
     6601 ttgaccatcg cagtgataaa cgctgttttg ccgctgcggc ttagtccggt tacggcgagg
     6661 cgcagatgtc tgtcgacacc ccgattcacc agcgcattaa gttcattttt aagtcgcttc
     6721 attgctgtcc tgtgctgctc tgaagaccag aaaaaaatca ttcgtaagga tatgaatgtg
     6781 ggggccaggc cattgattat caactgcccg tgttgtcatc gacgggcgaa ctccataaga
     6841 tgtgcagcat tcacgttcgg acggagttcc tgacctatgt cccctactat ttatgatatt
     6901 gccagggttg caggcgtatc aaaatccacc gtatcacgcg tgctgaataa gcaaactaat
     6961 atctccccgg aagcgcgcga aaaagtgtta cgggccattg aagaattaca gtatcagcca
     7021 aacaagctag cccgcgcgct gacctcttcg ggttttgatg ccattatggt gatttctacc
     7081 cgttcgacca aaactacggc gggtaatccg tttttctctg aagttttgca tgtcatcacc
     7141 gccaaagctg aagaagaagg tttcgacgtg atattgcaga cgtcgcacaa cccggcagaa
     7201 gacttacaaa aatgtgaaag caaaattaag cagaaaatga ttaaaggcat tattatgcta
     7261 agttcgccag cggatgagtc attttttgcc caactcgata aatacgatat tccggtggtg
     7321 gtgattggca aagttgaagg tcaatatgcc catgtttatt ctgtcgatac cgataatttt
     7381 ggcgacagca ttgcgttgac cgatgcatta attgaaagtg ggcatcaaaa tattgcctgc
     7441 ctgcatgctc cgcttgatgt ccatgtttca gtggatcggg taaatggtta taagcagagc
     7501 ctggctgcgc ataatattgc agtgcgtgat gaatggattg ttgacggtgg ttatacccat
     7561 gaaacagcac tgcaagccgc gagagaatta ttaagccagt caccgttgcc tgaggcggtg
     7621 tttgccactg acagtctgaa attaatgagt atttatcgtg cggcagcaga gaaaaatatt
     7681 gctattccgc agcagttagc ggtggtgggt tatagtaatg aaacgctgtc gtttatatta
     7741 acgcctgcac cgggcggcat cgatgttccg acgcaggagt taggtcaaca aagctgcgag
     7801 ttattattcc gcttaatttc tggaaaaccg tcaccacaga atattaccgt tgccacgcat
     7861 atgacgctga aataaatacc agctaccttg cctgaatgcc ggatgcgatg ctagcgcatc
     7921 ttatccggcc tacatttata gatcggataa agtactcgcg ccgcatccgg caatttagcc
     7981 cattatcaga acgagtaatt tacgccgaca cctgcataat ggaatttatc actgtctcct
     8041 tcgtcgtgat cctgccactc aaacgcgtat tccagcgaaa cggaaagtcc gttctggaaa
     8101 tcataaccgt aaaataaacc tacacggtta aaatcatggc cttcacgttc aatatcatcc
     8161 tgccagtccc agttactcca gcgatccagc ccaatgcgcg tatacggcgt caccgagtgg
     8221 ttgccgagcg tcagcggcaa ataggcgcga atttcttgcg tggaaaactc accgttattg
     8281 cggctgtcgt ccatattgaa gccgcgctcg agataatagt tcactcgcaa ggcaaccgtt
     8341 tcgttgaagg tatattgcag acctgtttcc gtttcgacac gggtatcagc gtaaccggta
     8401 gtgttcagat cgttggcaaa tttatacatc gacaaccaac cgttgaaacg taaatcgtca
     8461 gtcagtttca catcccagtc tggcgcgatt ttccagcgct gcatattcgc cgtgtcttta
     8521 cccggttcat caacgtagtg ataaccataa ttacggaaac cgccggtcag gccgaaactg
     8581 aaatcatcgt tttcgaggaa ctgataatgc acctccagct ccgggcgatc aaaccacgtg
     8641 taaccaggtg aatcatttga taccgacaag ggttttagtg aaaggaggtg aaatcacctg
     8701 taagatgctg aaataaaaca ggtgatgtat acttcggacg gaaaactctt tttgataccg
     8761 actgctcaac aaatagtcaa aagatcaaaa aacggcgaca catttgatac tgctaattta
     8821 cccttaaaat cttcttcaaa acatcttcaa actgccaaaa tgctcacata atcgatctaa
     8881 ctataatcag tatgataatc atcatgaatt actattcaat agcgtgatta tcttgggatg
     8941 agtcatatga tctctatatg tatttacagc accacctcat gatagcgatg gcgcttaacc
     9001 aacaacattt ttctttactg tcactgcccc tgattcctgg gtggcgttat tgctcgagtt
     9061 gtacttctca ataacgcgca aaatctcttt gtctgtttct tcacttcccg ccattcctat
     9121 ctttattgca ttttttagta cgggacgtaa tggcaaggca tcaatgatgc tttctaccgc
     9181 atcctggctg attgtagatg tattcattgc tgccagcctg acaaggttgt tagtaccttc
     9241 tcgcctgatg aatgtaagga ttaattgctt ctcttggggg cttagagagc taagcgcctg
     9301 atcaataccc gaacctgaat cgcaattttt ttcgattttt ccgcattcat cttcgttctt
     9361 gcctgtggcc aaccattcga gtgaacaatt acaggcttta gctattaagg ccagtttatc
     9421 caaaggaggg aagtttttcc ccttcctgta atttcttact gtggcttcag atagtccaca
     9481 aagggtcgcc agttcgacgt ttgtcatgtt tcccatagcc atagctaagc gttctgaaaa
     9541 tcggtttatt ccttccatat caataaaccg ttccgcactg gatacggttt tttctgcctt
     9601 atccatataa ccccctgtaa aacaaagaaa ttatcgacat cgcatttaat ttcgatatgc
     9661 tcgcaaaaaa ccgttgatat cgaattattt tgcgattatg atttacctaa atcgataacc
     9721 ggttttgatt atcggttcag gtaaacatga gaggatcaca caatgagggt atccagacaa
     9781 gcatcgaata atttgtttgt tcgagagggg tgtgactggt ctgctaaagc ggtatatgca
     9841 gcccttgaaa acaaaggcac agacctccct gaactggaaa gggagttagg attaaaaagc
     9901 ggatcaatgc gcaatgtgtt ttaccgccag tgccgtaaat acgaagccgc catcgccgaa
     9961 aaaatagggg ttgaacctgc tgtaatctgg cccagcagat accaggtgga cgccagagaa
    10021 gtggcttgat gggggatgta tgtgggtaac agcaaaagaa tgtgttggtc ttcctgattt
    10081 accaatgatg gaacataaca ttcgccatcg actcacaaaa gcagctgcgg gaaatgcagc
    10141 catgttacgc aaacgtaagg ggacaaaagc aatcgaattt aatatcgatt gtttgccttc
    10201 tggcgcgcaa caagtattgc gtgaacgtta tgcccttcaa ctgatgacgc aaaaagctga
    10261 tgaatcaccg gctccggtgg tgacaaaggc cagacgctca tctgccgtgg ttgatgcggt
    10321 ggaggcatat cgcggatcac cacaactgat gctcgaacgc ctcaatgccc tgactgaaaa
    10381 ccagcgccag gtggctgatg cacgaatcgc gatcgtcagc gaagttctga aagtcgcgca
    10441 acaacctggt ttcagctgtg cgaaggctat ccggtttatc gttgacaacc tggcacgttc
    10501 gcaactggac gagcgcattg tggcaatggt tgagacggcg aacgccaaaa agggaaacag
    10561 ccgtgcgttg agtgaaatca cgctgaaacg ctggattgca gcctttaaca aggcgcagaa
    10621 cgccgctgaa cgcctgcttt tgctggcacc tggtaagcgc caggaaataa aagccgaaga
    10681 tattaactgg ctgcccgaat ttctggcgca gtatcgccag tcaaacggcc gaccaatgac
    10741 cgaggcttac gaggattttg tcgctgaatg gcagtaccgg cacgctgatg agccttatat
    10801 gctcgatatc atgccgtctt atgacaccat tcgccgcgca atgaagaaac tgccggaagt
    10861 ggtgaaacaa aaaggccggg tgaccggcag tgaataccgc cagcttgagg gattcacgcg
    10921 ccgcgactgg tccagaatgc cggtgaatta tgtctggatt ggtgacggtc acggcatgaa
    10981 gctgaaatgc gcacacccgg ttcacggtcg gccatttgca ccggaagtga cctttgttat
    11041 cgacggtggc acgcgctttg tggtgggctg gagccttgac ctggctgaaa atgttttcgc
    11101 cgtagccggt gccatacagc acggcattcg ccatcacggc aaaccgtttc tgtattactc
    11161 ggataacggc tccggggaaa ccgccgacat cctggataag gaggttgtgg ggatactgcc
    11221 gcgactgggg attaatcacc cgaccgggat tgccggtaat ccgcagggac gaggcattat
    11281 cgaacggctt aaccgcacat taccgatgcg catagcccgt aaataccgca cctatttcgg
    11341 gaaaggtgca gatcgcgaga cgttacgcaa aaccaaccgc gatttacgct cggcatttac
    11401 tgccctgcaa cagggcaaac ggctgaacgc ccggcagcag tcagcgatgc gtgatttacc
    11461 gtcctggtct gaactgattg atgccattcg tgacggtgtt gagtggtaca acaaccggcc
    11521 gcacgatgaa ttaccggtga agccgaacgg caagcattac agcccggcgg agttcagaaa
    11581 aaaacgcctg gcggaagagg acacggaaat tgaatggctg tccgatgttg agctgcggga
    11641 tatgttccgg ccgatggtgg aacgccctgt aagacgctgt gaaatacgct ggctgaataa
    11701 tatttactac gcgcccgagc tgcgtgacga gcatggtcgt aaggtgctta tcagctatga
    11761 cattcatgat gccgaacgaa ttaccgtacg tcgcctggat ggcagcgtga tttgcgaggc
    11821 ggtatgggac gacaataaac gcgaagcctt cccggtcagc gcggaatacc acaaacagca
    11881 gcagcgcctt aaaggcatgc gtaaacgcgc agaggaaaaa atccgtgatg ccgaggatga
    11941 gattgtcaac gtgctggagc acaagccaca ggagccgtgg ctggaaaata tataccgccc
    12001 tgtgggtaat acggtgatcg ttcagcaacc ggtcgctgac gatgaacctg atgaagaata
    12061 cgagcgtaat ttccagcggg gattgcagct gcttgaagcg aaattaaaag aaaatgaccc
    12121 tctggcctga aataaaaaaa taacccgagc ggcgactcag gttatttgat taaacaaagg
    12181 aactacacaa acgcgaggta ataatatgac cgatataaac gatgtaatca agaccattga
    12241 tgaacttatt gatggcggcg tgctgacgca gtatgccatc gccagagagg cgggaatttc
    12301 cgacggcaca ttatcggctt tccgcaaggg gaaatataaa ggcgataacg ccgctgtggc
    12361 tgcttccctg cgctcctggt atgagaactg gaataaacaa agcgcactgc cggaaccgcc
    12421 gcagtttgtg gaaactcaga cagtccagga gctgcgtgca ctgtttcagg cggttcgcct
    12481 gatgggctgt attaacgtta ttgtgggcgt accgggtgtg ggtaaaacgg ccactgcccg
    12541 taattactgc caggagcaac caaacacctg gatgatcacc ctgtcacccg cgcactccag
    12601 cgtcacggag tgtctgctgg agctggccga tgcgctgggg attgattaca cccgcgcgaa
    12661 caaaggggca ttatcccgcg ccatccgccg tcgcctgatg ggaacgcgtg gactggtgat
    12721 tgtggatgag gcggatcatc ttggtattga cggtctggag caactccggg caattcagga
    12781 cgccacgggg atcgggatgg tgcttattgg taacccgcgc ggattgttta aaggtggacg
    12841 ccgcgccttt gatgatttat cgcgcctgtt cagccgtctt gcccgtacaa aacaacttca
    12901 caaggccaaa aaggcggatg tgctggccat tgccatggcg tgggggatca gtggtgaggc
    12961 cgagctggcc gtcatgcagg ctatcgctga aaagccggga gcgttacgcg ttctgacaca
    13021 tacgcttaac caggcgtggc tcaccgccag cggtgaaggc gcggcgctga cagaaaaaca
    13081 tattaatgcg gcctttaaag aggtttatac caaccctgaa ttactctcac aggtgtgatt
    13141 atggcggtat ttaatattcc tgatatttac ggacgctttt acctggttaa tttcgataac
    13201 gtgaaggtga tttcactggc cgaaaataaa gaatgtggcg atttactttt tgaatttaat
    13261 gaccgcacac gaatggtgat atctgccgga cttgatcgcg aaggtgcgac agaagtttac
    13321 agcggaatat gccgttctgt tggtgcgaaa caagtcagct aaatgaggtg ttatatgaat
    13381 atgcaatcct gcggtaacaa aatgaattta ttcgactccc tgaacagcgc gcgccgtctg
    13441 accgaacttg ccggtgcggt actggaacgc agtaagcgct atccgcaacg ttttgcactg
    13501 aaaaccacgc cgccggtagg caacgtgcag ggaaccggtg aaattgaaat caccatacag
    13561 accaacggcc tgcgccgccg tgtgaaggcc acccgcatca gcggctgcac ggtttactgg
    13621 gaggtgtgag gtgaaaaaaa atctcattgc atgggcgtgg tcgagtggtc ttattgagtt
    13681 tggttacgtc ctgccggaag gtgcattgcc gatagttgcc ggaaagcctg ccacggtacg
    13741 gcatgtgatt gaggttatgg cgcgtcatgg acgtgatgaa caggagcagt tactggttcc
    13801 ggggataccg gaagcggtga cggaggaaga agcctttaat gccatgattc ggttctgccg
    13861 tgaggttaga cgccgggtca gttatccaaa cagaacgagg accagaggat gagtaaagtc
    13921 gtacgcatta ttttcgaata caaggaagat gttatctgca aaaactctga tggcagcacg
    13981 cacagagggg taagtctgga tatacgttca accggaataa agaagaaaga taatggaccc
    14041 gccatgattt ttggggtggt tatgctggca gaaagcagaa acttcgcaga gtttgtggca
    14101 atgaaagcca gtgcattcat gaaagacaga ggcatggctt ccggggttat taacggtaat
    14161 gaatttaatc aacaggggta attccatgag caaagtacgc gttatttttg aatttaatca
    14221 tgtttcgcat gacgaaaagt tggcaggcaa tgactgtgtt gaagtgcatg aaaagattgg
    14281 agtggatgtg aaaacagaac gtgatacgga taacaggccg acgtcactct gtgacgttta
    14341 tgcaagtatt ctccagtatc acagtcctgc aattattcag tttctctcag cggaatttca
    14401 ggcatctgca cagtcttttg gagcggatgc catcattaaa cgccaccgcg tgcataaagc
    14461 atcaggcaca ctgcaataag gaaaaacaaa atggctaaac gcgttacaaa attaaaggcc
    14521 gcagccgagg cggcaccgca gacccgggaa gaagtcagcc gtgatatccg caccctggga
    14581 gatattcagc gagaggcgct gcgcctggaa acggcgatga atgacgaagt ggcagaaatc
    14641 accgcccgtt atacgccgca gattgaaaat cttaaaaagc aaatcaaagt gctttttaaa
    14701 gggattcagg actggtgcaa aaccaaccgt gatgagctga cgaacggcgg caaaaccaaa
    14761 acagccaatc tgaccaccgg aacggtgtca tggcggctgg gaacgccatc atgcagcgtc
    14821 agccgtgatg tggaaggtgt gattgaaatg ctgcgccgta tggggcttga gcgtttcatc
    14881 cgcacgaaag aggaagtgaa caaggaagcc gtcctggcgg agccggatgc tgtgaaaggc
    14941 attgccggta tcaaggtgaa taaaggcgct gaaagttttt atgtcgagcc ttttgaacag
    15001 gacgccggac tgaataaata acaccgcatt aatcaattaa atatcacttc attttaatta
    15061 tggcgctcgc gtcaggggac tgcttgcgcc tgaaaacaga aaataagggt taagaaatat
    15121 gaaatacgtt tatatcgtca ttaataatgt ggtggggaaa taaatcatga ttgatgcaaa
    15181 agtgcttgaa ggggttaaaa actggctgtg tatttatggc cgcctgacct gcggtgtact
    15241 ggctgaaaaa atgaatatgc cgccatcctc catggtttat tttctgcgtg atgcggttga
    15301 tgcaggcgtg ctgacggaat gtaacggttt ttatgatatt ccgcgccccc gcccggtgca
    15361 gccggttcgt cgcaaatgca gccaggaatc tgcggctgat gatgttcagt ggtgcggctt
    15421 cagaaaatcc ctgccatgga ttgaggggca tgatattccg tcgatggcgt gggaatttgc
    15481 tcagggcgtt ctgacctgtg aaaccgttta tgtggtggct gaagttgatg agcaggccat
    15541 gaaagaaggc gtgccccagt ttgtgatggc gtatatcgac atacgcctgg gcgtcattat
    15601 ctgcggttta agcggctgga atatcactga acatgttctg cgctacctga ttgttgaccg
    15661 gacagcagcg cctgccggga tatctgcgga ggtggcgtaa tgttctttaa aacatcaaac
    15721 ccttccgcgc tggctgcgtg gcaaaaatac cagcaggact gccagaaagt taaggatgag
    15781 gcaaaacgcc ttgaggccgt gctgaatgtt gcgtgccggt cggtatttgt atccggtatc
    15841 agtggatttt gctttaaggg actgcgtttt acggaggaca aatatccttt tcatcgcgac
    15901 ttatggcgaa aaccgactgc gtcgaatggc tggagctgca caccgcgcac atcacgtatc
    15961 cctaaaaccc tgcgtgttgc ctctgatgaa ctgaacagtc tgtggcgtga atattcgccc
    16021 gtcacgtatg ccagaaccga tgcgctgttg ttctggctgg gtattgattt ctcggcaatc
    16081 ctgtttggcc ctgtgaagtg gttctgcgtt gacgatgtga tttaccttca gtgcggcgta
    16141 aaacccgcaa aacagaaaat gaccgaaata ctgtctgatg agttttatgc tgctgaaaag
    16201 cgagtcaggg ggtgatgtat gatgatttta caacccatgg ggcgaaaagg tcgcgccccc
    16261 gctcatgtcc gtccgtggac acctgaagaa gatgcgctgc tgattgcgct ttattcatcc
    16321 accccggtta aggatattgc tgccagaata aaaagaactg tctgggctgt atataaccgg
    16381 actggtgtat tgcgcagttc atacccggag ttactgaaat ataaacaccc aagatttaca
    16441 cctgatgaag ataagtttat tcgaaaaaat gccagaacaa tgacctgcca gcaaatggga
    16501 gaatatctcg gacgtaataa agattctgtc agatgtcggg caggaatgat tggtgctgga
    16561 ttaacaaagt gcggagagtt acgccccggc acgcgcatat ctgatgatga tgtacgtctt
    16621 atacgtgcgc tgcgtgattc cgattaccca cgccgtctgt cattccggga aattggcgaa
    16681 aagtttggaa tatctgaaca ttctgctcac gcagtttatt accgtcgccg gactgccgag
    16741 gacgctgtat tacgggagtt aacgccatga taacgacctt atttgttgaa tcagatgaac
    16801 ctcttgtgtg tgccgccgga atgccgctct gtggcggaac gctgaccggt gtttatttcg
    16861 gggatttacg cggttatccc tggcattcac tgaatgatgc tttcccacct gatatggagg
    16921 ctgtcgtgct gattgttcag tatggtcacc gtcaggagct gcgcatcggc catatggggt
    16981 atgaaggctt ttttgttgat gaagaaaccg gagcctgcct tgaagatgaa gacggacagg
    17041 tgacgcactg gtgtcatatt tcggctttac cggaattacg ggaggtgaat gcgtgagaag
    17101 ttatattgat aatgaaaagc tggagacaat aagcgactgt cttagcttgc tggcaaaaat
    17161 caaagaaact attgaagaga ttaaatttca gcttgaatac gcaccatgtg gcgatgatgc
    17221 atggcgtaat gctgccagaa aagcactggc tgtctttcag aaacaacgcc gggctgttga
    17281 ataccgcctt gctgtattgc gccaggagga aaaggaaaga aatataagac gtcatgaacg
    17341 cgttaatgac ttccttgttc gtgaattaaa agggcgcgtg ccggagtcgg tattttttga
    17401 atgtgaggct atagctcgta gcaaagcact ggaaacagat taagcaggca ggtgaataat
    17461 gagtattgaa ttaagatcgt cttatgaata tcgcaaaatc cttatcgccg gagggatgaa
    17521 accggaagat gctgaaaaat tcgtttcttt tatggataaa gaatgtgaca agcgggatat
    17581 gccagaaatt attatggatg acatgattct ggattcagcc gtagcgttaa gtccgttatg
    17641 gattgtgcat acccttgcgg aaatagctaa aagaagtgac aagcaggcgg ctgtcgccgc
    17701 cctgcgatta ctggctgaat taagttgttt agtcaatccc aatacgttct ttgacgaacc
    17761 tggttaacat tgatttggta acttctttga ttagatcaaa tggcgcgtct gtaaactcct
    17821 ttttgattct ttcaaggatt ggtttttttc tcagggcgtt agctaaatca tgcccctgct
    17881 gagtaagcat aatgggcgtg tttctgaaac caacgcccag agaatgaaaa aagaaaccaa
    17941 tatattttgg tgtttctgac cgcatatcac cattaacaat tgagccatta tcagcgagaa
    18001 gcataagatg aaaaactttt ttatcctttt cctctccttc tgcctcagcc atgcccagat
    18061 cctgtagtgt aaggaaaggt gtttcagcat cctgaaaagt tgtgagaata cttaatactt
    18121 catcgtaatc tattttcata tcgatacctt tagttttatc cgggcggcgt tgccatgccg
    18181 cctgttgtta cggagacacg ataatgaatc gcgcttccct gattacctta atacatgtcg
    18241 caaaatgcga tctacagctt gaccatgaga cctacacatc cgtactactg gcagccaccg
    18301 gcaaacccag ttgccgggat atgtcaccgg atgagttatc ccgcgtgctg gatgttttca
    18361 aaaaacgcgg ttttaaagtg cgtcagaagc cggttaaccg ggccttaaaa ccgggtacgg
    18421 tgaccgccaa aattcgcgcc atctggaagg tgatgcaccg gcagggcttt atctctgatg
    18481 gcggggaaac agcccttaac cgctgggtga aatcgcagac ggccgctcag aacggcggcg
    18541 aaggtgtggc taactggcag tggctggaac aacaccccgc gctggcctct gatgtgctgg
    18601 agcgtctcaa acgctggcac cgccgcaaga tgctggatgt gctggggatg tcagcgcgta
    18661 cagtgatgag ttatgagcgc gtttgcaggc tgtatgaaga atcctccccc ttttaacccc
    18721 aaatcccgcc acagcgcggg atttttattt taaacttgcc ctgtgtaatg atgaccggag
    18781 gcgggtatgg ctgaaacaca gatgagcatg tttggtgaca gcgaacagct gcacgcgctg
    18841 attgaccgtc tggatgatat tcctgatgat gagctgaaaa agaactggcc cggcacgctg
    18901 cgggacctgg ttgatgtgat cagtgctgaa ctgcaccgtc aggggattga acctgctcag
    18961 gcgggaatgc ttgcccgtaa ggttgcagcc gcacaggccg ggtacatggg cgggcgtggt
    19021 tattatctcc cggtcggtga atcccttttc accgaactgc gcaataatga aattttttcc
    19081 cgctggagcc agggcgaaaa aattgaaacc ctgcgccgcc actaccagat gtcagagacg
    19141 cagatttact ccgttatccg caaccagcgt cgcctgcatc aggaacgcac gcagccacgg
    19201 cttttctgat attccgcaaa tcctgcccac tttttcccac ggttacgctg actcagagaa
    19261 caccatgaga cagcgtaaca atgccaaaac tccccgcacc cctgcgtaaa aagctgattg
    19321 cccttgttct ggccggagcc gggacgttca ccattgccac gcactacacc ggctactggg
    19381 aagggaaaga aaacaccacg tacattgatc ccaccggcac ccccaccatc tgttacggcc
    19441 ataccggccc ggatgtgaaa ccggggatga caaaaactga tgaagaatgt ctggagctgc
    19501 tggaaaagga catgaaatgg gcctttgcgg ctatcgatcg tcatgttcag gtgccgctta
    19561 cccgtggtca gacggtggcg ctggcctcct ggattttctg ggccggtgaa acgaactttc
    19621 gcaactccac gctcctgcgc cttatcaatg ccgggcagat gcccgggtcc tgtaagcagt
    19681 atatccgctg gatttattca aagggggtaa aactccccgg tcttgaggcc cgccgttcgg
    19741 cggatgaatg gttgtgtcgc tacgatttgc cgaaagtctg aaccgcttct ggcgaccact
    19801 catgattgcg ctgttgtgtg tcgttgtggt gctccggggt gtgctgtggc tgcggtgaat
    19861 aatcccttac cggccctgat tgccggaggg tgtatggcgg cgctgggggt gatgagcgtg
    19921 ctggctgccg tggtatgggg aatgcaccag aagacacagc gtcttgaaga caataatcat
    19981 gtgcttgtgc gtgaacggga cgaagcccgt ctggtgctgg caaaccagca acgtaccctg
    20041 caactcattt cacaaatcag taaggcggcc acgaatgaaa aacagcagaa catgcagcac
    20101 agcgaggaac agcagagcat tgtccgccgg tcgctggcaa cggtgcctgc ggcctctgtt
    20161 cctgtgcctg atgatgtggc tgaccgggtg cggcgggccg tctgtgaaat acgtgccggt
    20221 gaagccggtg ccgatccccg ctgaatggct ggctgactgt ctggttcctc ctgtgccgca
    20281 gccgttcacg tttggcgcat cggtcactta caacctgcaa ctgctggcgg tgattaagaa
    20341 ctgcaacgtg gacaaggcca gcatccgccg tctggaggca cagcgacagc atgaatttac
    20401 tgatatggcc ggaacgactg ctgttccggc aggaaagacg aagtaaggaa aaaggtatgg
    20461 atgattcaga ttgtgctcag gcagtcgtgg agcgggcaac agaacgcgcc ctttgtgacc
    20521 ggctgacacg taagtgccgg gtggaggaga ctccgggcag gcgtgtatgc gcagactgcg
    20581 ggggtgacat tccggccgcc cgtctggttg ccgtgccgga tgccatccgt tgtgtgaact
    20641 gccagagcat catggaggcc ggtcatgtgg gtcagcatcg ttaaagatta tgtcgtgccg
    20701 atcctgtcgg caacagccac ggccgggggg atctttatgg cgctgatgcg caaaaccttt
    20761 gtcccccgcg aggcctttga aaaactctcc gaccgggttg agcgggtgga gagccggatt
    20821 tccggcctgc ccacgcagca ggaggtgaac agactcaata tcgaaatcac caccctgcgc
    20881 ggtgagctta aggccaccaa tgccaccctg aaatccgtct cccaccagaa cgaactgctg
    20941 ctggagcagg ctgtaaggaa aaaacacaat gagtgatttc attactgaag atcagcgtct
    21001 tgtcattctg cggtcgctgg cggattacaa cggcgaactg ggcgaatccg tgcttcagga
    21061 ctgcctggat gattacggcc accgcgtgtc ccgcgacacc gttcacaccc acattgcctg
    21121 gcttgctgag cagggactgg tgcgtaagcg cacgcttgtt aacggttatt tcatcgctga
    21181 actgaccggc cggggtcagg atgtggcgga aggtcgtgcc actgttccgg gtgtgaagaa
    21241 accacgcgca agggggtaac gatggataag ccgacgcgtg gccgtgtacg caaggtggat
    21301 ttgctccccg acagcatccg taagccgctg ctggaaatgc tgcgtgaaaa acgcctgacg
    21361 caggtccaga tccgcgagga aatcaaccgt ctgatccgtg aggcaggcct gccggaagag
    21421 cagcagcttt caccggcggc catcagccgg gaagcctccc gtaatgagct gattgcccgt
    21481 aatctgcgtg atttacgcga gcagacaaaa gccatgatgg ccgaactggg ggataagccg
    21541 accggtgaaa ccacggcact gattctggag atgtcccgtg ccctgatgta ccgccgtctg
    21601 cgtgccgcca ccgaatccct gaacagcgac agcgatgtgg atatgcgcct catcaaggac
    21661 atcctcctgt cggcacagcg tgcagaaagt gccgccgagc gcagtattaa gcgtgaaaaa
    21721 gaaatccggg ccgcatttgc tgaagagatg gcgaacgcgg tcaccgacga gttacgcggc
    21781 gtggatggca tgagcgagca gatcgaattc cgtatcaggc agatgctgct gggtaaggca
    21841 taagaggtga atcatggcaa cagataaccg ctcactgatg agtagtgctc atctgaaggc
    21901 attcagtctt gcaacacagt tacctcctga aaccataaaa gcgtgtcgtg agttgactga
    21961 actcagcatt gccggggaaa tatccctgga acaactgcat tcgctgctgg cagaggctgc
    22021 cactgatgag ttgagcggcg tggacgtgat gactgaagag cttgagtccc gaatcaaaag
    22081 cattctgaca ggtaaagcct gatgaacaaa gccaaagaaa agtctccttc actgatacaa
    22141 atgacgcctc cccgcaaaat tgatctgtcc ggtgaaaaag ccctgctggg cgtggatgtg
    22201 ccggacagcc ttgatttgcc cggtgatatg ccggtgtttc tggattatca ggcccgctgg
    22261 tttgcggatg aaagtcaggt ctgcatcgcg gaaaaatccc gccgcaccgg gcttacctgg
    22321 gcggaagccg ggcgtaacgt cattaccgct gcaaaaccga agcggcgtgg tggccgcaat
    22381 gtgttttatg tggggtcaaa acaggagatg gcgctggaat atatttctgc ctgcgcgctg
    22441 ttctcccgtg cctttaacca gctggcagat gctgatgtgt atgagcagac attctgggat
    22501 cgggataaaa aagaagaaat tttgacctac atgatccgct ttccgaacag tggattcaaa
    22561 attcaggcat tgtcttcccg tccgtcaaac ctgcgcggcc ttcaggggga tgtggtgatt
    22621 gatgaagccg cgttccacga gtcgctggat gaacttctca aggcggcaat ggcgctcact
    22681 atgtggggcg cgcgcgtgcg tattatctcc acgcataacg gcgtcgataa tctgtttaat
    22741 cagtacattc aggaagcccg cgaggggcgc aaggattaca gcgtccaccg cataaccctg
    22801 gatgatgcca ttgcggacgg gctgtaccgt cgtatctgtt acgtcaccga tcaggaatgg
    22861 tcaccggaaa gcgagcagaa atggcgtgat gatctctaca aaaacgcccc gacccgtgag
    22921 gatgccgacg aggaatacgg ctgtatcccg aaaaaatccg gcggtgccta tatcccccac
    22981 gcgcttattg aaatggcgat gatccgcgac atcccgattc tgacgtttga agccccggac
    23041 aacttcatca gccgtgccgc atggctgcgt gaatcggaag ttttaacctg gtgtgaagaa
    23101 catttaaaac cgcttttaga gaaattaaat ccccgttccc gcttcagctt cggggaagac
    23161 tttgcccgca ccggtgacct gtcctgcttt gtgctgctgg aaatcaccga atccctggca
    23221 aagcgcgagg tgtttcgcgt ggagctgcgc aacctgccgt atgcccagca ggaacaggtg
    23281 atgatgtaca tcctgacccg cgttccggcg ctggtcggtg cggcgttcga cgccaccggt
    23341 aacggcggct atctggcaga ggccgcactg ctggcctttg gcccggacat catcgactgc
    23401 gtgatgttgt cgccgaagtg gtacggcgag tggatgccaa aactgaaagc cgagtttgaa
    23461 gatcagaata tcctcgtcgc ccgccatcag accacgcttg atgatttgcg ccatgtgaag
    23521 gtagtgaatg gtatcccgca aatcgacaag gggcgcacga aggaccagaa cgcaacggct
    23581 gcgaacgccc gccgccatgg tgattttgcc gtggcgctct gtatggcaaa ccgggcgtca
    23641 tacatggagg gcttcatcct ggatgaatca gcctgtcagg cgctaccgga gcggtcgcgg
    23701 gcaatggagg gcggttatcg tgatgacgat gaggcatatc atgaatttga tcgggggggc
    23761 tggtagtggg acgcatcatt gatactgacg gaaaaccttt ctcctttgac ccggagatgc
    23821 agagtgccgc gctggatatt ccgcagattg ccagccgtta tattgaacat ccggcttcgg
    23881 gtatcacccc gaaccgggcg gcgcagtgcc ttcgcggggc tgaacgtggc gatctgattg
    23941 cccagtccga tctggccgct gatattgaag aaaaggacac ccaccttttt gcggagctgg
    24001 gcaagcgacg tcttgccatt cagggggtgc catggagtat tgagccgcca ccgaacgcca
    24061 gcgcgaatga gaaaaaggac gcggaaatgc tcgacgaata tctgcattcc gcagactggt
    24121 ttgatgccat gctgtttgac gccacggatg ccatcctgaa gggctattcc tgcatggaaa
    24181 ttgagcacgg gatgctcggt aaaatgcaca tcatccgcgc catccgctgg cgtgacagcg
    24241 ggcatttctg ccttaacccg gatgatttga gcgaactgcg gctgcgtgac ggcagccatt
    24301 ccggggtggc gtttcagccc tttggctgga tagtgcatca gtcgcgttca cgcaccggtt
    24361 acggtggtgc aaccgggctt gtccggacgc ttatctggcc gttcattttc aaaaactatt
    24421 ccgtgcgcga tctggcggaa tttctggagg tgtacggcct gccgatgaag gtcgggaaat
    24481 acccgtccgg ggcaacaccg gagcagaaaa gtgccctgat gcgggcggtg atggatatcg
    24541 ggcgacgtac aggcgggatc atcccggccg ggatgtcgct ggagtttcag gcggcagcga
    24601 acggtcaggc cgatccgttt gaaaccatga tttcgtgggg ggagcgttcc atctccaaag
    24661 caatactcgg cggcacgctg accacggaag ccggagacaa aggcgcgcgc tcactgggtg
    24721 aggtacataa cgaggtgcgc cgggaaatca gggattctga tttacgacag ctggctgcaa
    24781 ccctgaaccg cgatctggtg tatccgctgt acgccctgaa caccacgcac actattgata
    24841 tccgccgtct gccacgtatc tgcttccaga caaaagaacc aggtgatatc accaaaatca
    24901 ccagtgcggt gatgcagctc agtacgggga tggatatttc tgatccctgg atacgtgagc
    24961 agaccggtat tccgcagccc acgcccggtg aagccatatt ccgtgtccgt cagagtggca
    25021 atgaaccggc tcagaccgac agggaaacac caccggaaaa acaggaaaaa acagagcaga
    25081 cggcgctgtc agccagactg ccggaagcga aaagcacgcc gcgtgatgaa ctggatgata
    25141 tgggggacgc ggtgcctgcc cgccgcttac aggaggccat cgacccggta ctggagccgg
    25201 tcattgatgc catcagaacg cgggggctgg cggatgcgct ggcagacctg cctgcccttt
    25261 atcgtgaaat ggatgattcc cgcctgatga cgctgctcag tgatgccatg tttgctgcgg
    25321 aaatgaaggg gatgctggat ggctcaggag attgatttag gttatgccgc cacgcttccc
    25381 tcaaaagaga cggtggcata cttccgcgcc aaaggggcgc atatcagctg gaactggttc
    25441 gaaacagccg cggatgttca tgcccgctca ttcacggcag caaaagcggc acgcctggat
    25501 gtagtgacca cgttacaggc cgaagtgcag cgggccattg atgagggaat ttcacagaaa
    25561 gcatttatcc gcacgctgac gccacgcctg caaaagctgg gatggtgggg aaagcagatt
    25621 gtggtggaca gcgacggtaa cgcggaagaa gtgcaactgg gcagtccccg tcgtctggcg
    25681 ctgatttaca acgtgaacac ccgggtggct tacaatgccg ggcgttacac gcagatgatg
    25741 aacaacacgg acacgcatcc gttctggcag tatgtggcgg tcatggacag ccgtacccgc
    25801 ccgtcgcatt ccgcccttaa cggtctggta ttccgctatg acgatccgtt ctggaaaaca
    25861 cactacccac ctaatggctg gaactgccgc tgccgtgtgc ggccattgtc tcaggctcgt
    25921 ctggatgcaa tggggttatc cgtttcatcc ggtgaggatc atctctccac ccgcaatgtt
    25981 gaggctggcg tggataaaca gaccggagaa gtcagagaaa tgccggtgac cacatattca
    26041 gatggcacca gaaccatgac accggatgtg ggctggtcat ataaccccgg ctcggcagcg
    26101 ttcggcacag accaggcgct gatccgcaaa ctgatcgagg tgaaaagccc ggcgttacgg
    26161 gaaatggtgg ttcaggagat gaacaacagc ccggagcggc agctggcgtt ccgcatctgg
    26221 gcaaaaaaca tcatgcagac ccggcgaggt ggtaacgata tccgcacgct gggctttatg
    26281 actgaaagca ttgcgcaggc agtggaaagc cggacgggaa cgccaccggc ccgcctgctg
    26341 gcgatgagcg gtaaaaatgt gctccatgca gacagtgtga aacatcagaa tgacggcatc
    26401 gccctgatgc cagaggactt cgggcgctta ccggcaatgc tggcaaagcc aaaagcggtg
    26461 ctgtgggaca aacggcacaa aaatctgatg tatatcgtgg aaaataagga tggcagtgtg
    26521 cagattgccg ttaatgcacc gtacagtctg aaacgtcagc ccgacaaact ggatgtgatt
    26581 gtgaatgctt accgggttat caatatggat aagctgaaat cggatatccg gggcggaatg
    26641 cttgaggtgc tggaaggaca aattgattaa cggtcagtgg cgggggtcga acccgcatac
    26701 atgatccacc cggaggcagg caccgacttt accagttaag cgtacactga ccgcctgttt
    26761 attttatatt catcctttat gcggaggcaa tatgtcatcc attgatgccg ctgtcgttgt
    26821 tgacgttacg cgtctccagc gggtgtttgc ccggcttcag tttgtgggcg gcggaaaaga
    26881 cctggcccgc agtgtggcgg agagtcttct gtcatcgtca gaaatggcgt ttgaacagga
    26941 aaaagagccg gacggcgaac gctggcatga ctggtcagat ccttaccgca agtggcgtac
    27001 ccgtaaggga tacacgcccg gcaaaatcct gacactgaac ggcgatctgg cccgccgcct
    27061 gaccacggat tatggcgata cctgggcgct gattggatca aatgaacctt atgcggccat
    27121 tcatcagtgg ggtggcctgc ctggtatgcc accgggaccg gcggccattg gcgcacgtcc
    27181 gtatatgggc tttgatcagg tggcagaaca ggagatcatg gacgaaatca gaaaacgctt
    27241 taaaaaggcc acagaaacgc cgtaatggtt taaggtatac aaacgcattg ccttaccctc
    27301 ttcaggcgcg tgtcgtgatt ttctaacctg tatttaacgg gctttaaaat ctgcgtgaag
    27361 cgcctttctc atttttcccc tgtcagcact ttctgaagtc ccgcaaaacc ggccccgaaa
    27421 aacaccggct tatgctgccg gaatgaagac gaaaaacacg cccaaacttg cttatgccat
    27481 tctgaatgcc atcagcctgt ccgcagacgg ggacggtgac tggtgtcaga tcatgccagc
    27541 cggtcgggta aaggcccgtg acggtcgccc ggaaaaaccg gcggaaggct ggctgattaa
    27601 ccatgcggcc gttgagcgca tggtatcccg ggttgtggcg ctcaatcagc cggtaaaaat
    27661 tgactacaac caccagaccc tgattaaggg gcatccggca ccggctgccg gctttgtcat
    27721 ggcctcaccg gaaaacttcc gcttcagtga agagcggggt tttgaagtgc gtccggactg
    27781 gaaccctccg gccattgaac acctgcgcaa taacgaattt ccctggtttt ccccggtgat
    27841 tggttatgac gaaatcaccg gtgaacctgt tgagcttcgg atgctggcta ttaccggtga
    27901 ccccggtctg actggcatga atcctgtcgc cgcactgtcg gcggatgacc tttataacgc
    27961 cttaaaccct cctttaaagg acacctccat gaatgagcaa ttacgccagt tgctgacggc
    28021 gctcggtctg accgtggccg acggtgacga atttacaccg gaactgggca cggcggcgct
    28081 gtctgccctt accgggatca aaacccgtgc ggatgcgcac gacaccctga aaacgcaggt
    28141 tgccagcctg tcggcagagc tggaaaccgc aaaaggcaca ccaactggcg gcactattga
    28201 tctgacgaaa tacgtgcccg ttgagacgta taacgccctg cgtaccgaat atgtcgcgtt
    28261 gtcggcacaa cacggcagca ccacgctgga gcagttgctg gataaggccg agtccgaagg
    28321 acgcattttc aaaagcgaac gcggatacat ggaaggactc ggacaacaga ttggtgttgc
    28381 ggcactttct gcacagcttg acgcccgcca gccggttgcg gctctgacca ctcttcagac
    28441 cgacaccgta accgtgccgg ataaaaagac cgccaccgct gcgctgtcgg ctgaagatat
    28501 cgccgccgcc catctgctgg gtaaaaccga agccgagttc ctgaaaatga aagaggaaat
    28561 gcaataatgc ctaccccgat tacaccggcg atgatcaccg ccctgatgac gggctaccgt
    28621 tctgattttc aggccgggat gtccatggct ccgtctcagt accagaaaat tgcgatgacc
    28681 gtaccgtcca cgtcgaaatc caacacctac ggctggctgg ggcagttccc gcagttccgt
    28741 gagtggatcg gctcccgcgt catcgagaag atgaaagcct atggctacgc catcgtgaac
    28801 aaaacctttg aaggcaccgt tgctattagc cgcgatgact ttgaagacga caatcttggc
    28861 atctactccc cgttgttcca ggagatgggg cgcgctgcgg cggcacaacc ggatgaactg
    28921 gtctttgctg cactgcgtga tggtctgaat gcggcctgct atgacggcca gaacttcttt
    28981 gataccgaac accctgttta ccccaaagtg gatggctccg gtgatgcaca gatggtcagc
    29041 aatatgtttg tggcaaaaac cggctctgtc ggtgcacagg cggattacag cggcccggcc
    29101 tggtatctgc ttgactgctc ccgcgcggta aaaccgctga tttatcagga tcgccgcaag
    29161 gccgaactgg ttgcccagac aaaagtcgat gaaggccgtg cgtttactga taacgagttt
    29221 gtgttcggtg cttccgcccg tcgcaatgtg ggctacggct tctggcagat ggcctacatg
    29281 atgcagtcac cgctgacgct ggatgcgctg tggcacggct ggtcagccat gcgtgacttt
    29341 aaggctgacg gtggccgcaa actcggcatc aaacccaccc atattgttgt ccccacctcg
    29401 ctggaaaaac aggcggtgca gctgctggag cgcgagctgt tcgcggacgg taacaccacc
    29461 gtctctaacg agatgaaggg caagctggag ctggttgtcg cggattatct gtaaacggtg
    29521 agccgggctt acggcccggc ccctgcggag gacaaatatg aatgaacatc acactgtggc
    29581 agcaacagat gattcaggcc ttcaggtcag tggtgacagt cctgtcacgg tactggcaga
    29641 agtgcgttgc agtcgtgcgc agtttcgtcg tgcggggttc ctgttcatgc ggggacgcca
    29701 gcaggttgac gtcaccccgg agcagcttgc ccgactggaa gcggagccat gcctcactgt
    29761 acgaatacta cagacgcctg gtgatgatgc gggagccgtg gcgggtgtgg ttcatgcaga
    29821 gaccggtgca gatttagccg aagccggaca ggatgctccc cggaaaaaga ccggcaataa
    29881 cgccagacag gcgaaaaagg ctcgcgcatg aattacgcca ccgaaaccga tatgcgggca
    29941 cgttaccgcg aggatttgct caggccatta ctggccgtgc cccgttcgga tgagccggac
    30001 acgcgcaagc tgaaccgggc actgacggac gcgtctgccc ttatcgacag ttacctgtct
    30061 gcccgttaca cgctgccgct ggcggtggtt ccggcggtcc ttgttcagca ttgctgcacg
    30121 attgcctttt attacctgtg cgatcagcgg gcatccgatc aggcgcgtga ccgttaccgt
    30181 gaggcgctgg cctggctgaa agatgtcatg aacggtaacg tgccggtcgg cgtggataca
    30241 gacggtgcgg cccctgaatc cggggattta ccgcaggtgc agtctgatgc ggcggtgttc
    30301 gggcgcaacc agaagggctt tatatgatta cggaaaccga gcaggcgtac atcgcccgta
    30361 tccgtgagta tttcgggaat gaactggtgt ctgttgacac acatcccggc gactggagcg
    30421 acagcgtgct gcgcaccatg ctgattaacg ccccggctat ctatgttgcc tggctgggtg
    30481 ccggtgaagg gcgtacccgt gggcgtctgg tcagtcactg ggtgttctac gtcatcggcg
    30541 acatgctcaa tggccgtgag gtcagccgtc ccgggcttta tcagattgtg gcccgcctga
    30601 ttaccgtgct ggacggcttc agagcagaaa aaacctcacc gctttacttt gaaaaggcgg
    30661 tcaacggtta caccgaaacg caggcaggca gcggtgccgt gatgtacgcc ctgtatttct
    30721 catgcgagga aatgatcgac ccgctgacgg atatcagctc gctggatgat ttcctgcgcc
    30781 attacgaaac ctttaccgaa ccggaaggca cgccggagtt taaggcacac atccgtctgc
    30841 ctggtgcaac tgccgccggt gaaaacgccg gaccgtcaga ggaataacag atgacaacca
    30901 tttttattaa acctgcgccg ggatgcctga ttcgcgatcc cgacacgatg actccgctct
    30961 cacaggacgg tgaagagaaa cccttcactc cgttctggtg ccgccgtctt aatgatggtg
    31021 acgtcattgt ggtggataaa acggaggagc ctgccccggc tgccacaaca ggtaataccg
    31081 gcgcggcggc aaaaccagcg ggagcggtag cagaaaaaac tgcggcggtg gctgaaaaaa
    31141 acgcagcatc agataagggg acgtcctgat gattaacttt gattatatcg gcgataacaa
    31201 tcgcattccg ctggtacaga ttgaaatcaa taattcaatg gccgtgaccg gcactccacc
    31261 acagcgtcag gcggtactgt tgtttggtca ggctgcaatg aaagacacca ccattcaggg
    31321 gcgcggtcag cttgatgtac cagtacgtat tacccgagct tcacaagcaa gagaactctg
    31381 ggggcgaggt tccatgattg cactgatggt agaggaattt atcgccatta accctgacac
    31441 agagttatat gccattgcac agggggctgg aacaggtcag tccagggcat gcgtgatgaa
    31501 tatcattgga acagcattgg cagacggggt gttaagtgtt tacatcggcg gtcgccgtta
    31561 cctcctgccc gtcagtaagg ggaaaaaagg taaagagctt gtggaagagc tggctaaggt
    31621 cattaacgct gacactgatg cgccattcac tgctatcgca gctgaagtca gtggtgataa
    31681 tgctgaagga cttaaaggca gcatgggggt taacgcccgc tttatcggtg aatgctcagc
    31741 tcatgattta cgggtgaact attatgacgg tgaaactaca ccggccggtc ttacgctgga
    31801 gctcgtcgcg ccaacacaga aggcaacaaa ccccgatatc acccgtagcg tctcagggat
    31861 ggggaatcgt cagtacaact acatcgtcat gccctataag gatgcggcaa atctcaaaat
    31921 tctggcggat gaactgctga aacgctgggg accggtaaaa atgtcggacg gtatggtctg
    31981 gatggcgcat accggcacct tcggtacggc aaccagtttt ggtgaatccc gtaatgactt
    32041 cctgtttacc tgcacttcta ttcctaaagc accagagccg gattatgtct gggcggcctc
    32101 catttgtgcg gtctgtgcgc cctccctgtc aacggatccg gcccgaccat tacagacact
    32161 ggcgctgcct gcccgtatgg cacctgaagc accggctcgt ctgacgcggg aagagcgaaa
    32221 cagcctgctt tataccggca tgtccacggt gaccgtcgcg gccgctgaca tggtgcagat
    32281 tgaacgtcag gtgacgatgt accgtcagaa tgcatacggc gaaagcgatc ccagctatct
    32341 ggatgtggaa accatctaca ccctgtcata tctgcgttat tccctgcgca cctttatcac
    32401 gcaacgtttt ccgcgccata aactggctga tgacgggacg cctgttcgtg caggtcagaa
    32461 tatcgtgaca ccggagatca tgaaacttca gttaatttcg ctgggtgaag aatgggtgaa
    32521 gctgggactg gtcgaaaatc tggacacctt taaaaagaat ctgctcgtgg agcgcaacac
    32581 aaaagaccgt aaccgtctgg atgtgatgtg tacaccggat ctggttaacc agttccgctt
    32641 catggccgcg cagatccgtt tcattttgtg aggtaaggca tgagcggaaa acagtatcag
    32701 ggcacggcca ccatccgtgt gaacggacag gagtacgcca cccttgaggg tgccacgttc
    32761 tcgccgtccg gctttgagcg tgaagtggtg aaaggcgcga aagtctatgg ctatcgcgag
    32821 aaaccccgtg aggcgacgat ggactgtaaa tttccggcgg gcggagaggg ttcaccggct
    32881 gccgatgaaa tcaacacctg gactgcggtc acgattgagt tcgtggccga taccggtgaa
    32941 gtccacatga tgacgaaggc ctggagcagt gaaccggcct cgcttgacgg tggcggtgat
    33001 atctccgcga agttcgccag cgccaccagt acccgtgtcc agtaatcagg aaaaatgtaa
    33061 tgagcacacg taagaaaaaa acggcggttt ctgacgaggc cgtgatggag gctatccggg
    33121 atgcacttga agacggcgat ccccgcaccg ccgggctggc tgaacaactg gcgaacggct
    33181 atgtggatct gctcgacggt ctgccgttcg gggaatcccg tgaataccgc gtcaccttcc
    33241 gcgaactgac ggcgaaagac agcattgatg cggagagcga ggccgagcgt cttatggaaa
    33301 cccaaaacgg tccggttctg gtggcctcac cggcactgcg cggcattgcg ctgctgcgcc
    33361 gtcagattgc cgctgttggc cagattgagg ggccattgtc tccgcgtcag attggacaac
    33421 tcagcgagcg cgatctctcc cgtctgatgg cggcggtaag cctgcttgac tcggcgatgg
    33481 ccggaaagct ggtcgttgaa cgggggcgaa cagatgcagt gccgggatca gattgaagaa
    33541 gccgcaattg tgctggggat ggtgacaaaa agcggcccgg agtgggcgct taacctcccc
    33601 ttatcgcagc tttaccggca ctgccgacag accgaaaaaa ttctcagaac gaagcagtaa
    33661 cccatggcaa aaaacctgaa agcctccctg attgtcgatt tactcggcaa tatttctgcc
    33721 aaatcccgcc agtggtcaca ggagctgggg gcattctccc gttccggcag gacggggctg
    33781 ggtggtcttg gaaatgcagc acgtcgtgcc ggtcaggaga cagaacttgt cggctcccgt
    33841 atgcagcgaa cgcttgccgg tgttcgtggc agtatccgca cggtgacgtc agattttgac
    33901 cgccttcagg gcagcattac cggcaccatc gggcggataa gcaaccttta cgggatgctg
    33961 gcgggagggg ctgcggtcta cgggtttaat aaagccttta tccgcccggc cgcagagatg
    34021 gagaactata ttctgcgtct caacgccatt aatcatggcg atacggcaaa aacggaagca
    34081 gtgaaagcct gggcggtaca gaacgccaaa gacaccacct gggggctggc gggggttatg
    34141 caggaatacg cctccagtcg cgggttcggt atgagtgaca gggaagcccg tcgctttatt
    34201 accatgcttc aggatcaggg cggctatcac ggctggtcac tgtcagatgc acagggtgcg
    34261 tccctgcaac tcaaacagat gtttgcccgc cagagtatcc aggcagcgga cgccaatatt
    34321 ctgaccggat acgggattaa cgtttaccag ttgctggccg ataagctggg tgtaaaccag
    34381 aagatcatcc gtgagaaagg cgagaaagga aaactgggac cggacagcat tcgcctgtta
    34441 tttcaggtga tggccgaaca ggcaaaaggt gcccagaaaa acgccatgaa ttcctggacg
    34501 ggaatgacgt ccatgatggg cgatgtctgg gacggatttg cccgcgaggt gatggcaaag
    34561 ggaccgtttg acagcctgaa aaagagcctg aaaggctttc tggattatgc tgatgcggca
    34621 cagaaaagcg gcttacagga taagctggcc acacagacgg cctcagccct gaatcaggga
    34681 ttcgagtatg ccagagacgc tgcaaccggc ttttaccggg ccattcagaa agtcagggaa
    34741 acgcttcagg cactgcgcga cgccggttat ggcgatgcac tggatcgtat tggtcagggt
    34801 gcgcagaccg ccgcaaagta cctgatgtat atgtatctcg cctcccgtgc gctgaaggtg
    34861 ctcaggtttg cggggacggg ggcattacgt ctcggcgcaa cccccttacg ctatggcatg
    34921 gcgatgacct ccgtgctgac gtcgcccttc cgcaaaccgc agaccactgt accgggaacg
    34981 cagcccggac gcgctggccg gttcctgaat ttccttaccg gggtaaatcc ggctgccgtt
    35041 cagcccgtgc tggtcaccaa ctggcctgcg ggtggcctgg cctcaggtgg cggggatgtg
    35101 gtggtcagcg gtgacggcaa aaccgtgcgc ggccgtaaaa aacgcggccc cggacgtgga
    35161 cggggtgtca cgaccgtcgt gactgccggt gagcaactgg cggagagtgc cggtaaacag
    35221 ggcttcttcg gtcgtatgat gagccgtgcc ggtggtctgc taacggcggc cggaaaccgc
    35281 atggggctgg ggcgttttgc cgggctgttc cgtggtgccg gtcggctggg tggtggtgcc
    35341 ctgtgggccg gtgccatggc ggctcctgta ctgctggaca gcagcgccag tgcagccgat
    35401 aaagccggtg ccgtgggttc tcttgccgga agtatcgccg gtggcgcgct gggggcggct
    35461 gccggtccgg tcggggttgc cattggttcc acggtgggca gctatctcgg tgattatctg
    35521 gggggctggc tgacgcaggc ctggcagaaa ctgcgtggca gcagtgacga aaacggcgga
    35581 caggccaccg cgaaaaccgc cgcacgggtg gagcttgtgg cccctgaagg ctggcgggcg
    35641 cgcagtattg atgtggatga taccgcacag catggcctgg atgtgaacgt ctggaacgga
    35701 gggaactatg gcctctactg acacgccggg gcgtggctcc tttcgtggcg taccgttcct
    35761 ggtttatcag gagcagcggg aacggggcgg acgtaacatt gtgcgccgtg aatacccact
    35821 gcgtgaaagc ggcggtgcgg atgatctggg gccaaaactc ccggagttca cattcacggt
    35881 tctggtgacc ggagacgatc ttcagacaca gcgaagccgc ctgcgtgatg cgctgcgtgc
    35941 acccggtgcc ggtgaactga tgcacccgga ttacggcacg ttaaacgtgc tgataaacag
    36001 ctttgaaagc cgttataacg ccagcgagca gggaacggtt gagttcacga tcaacgtgat
    36061 ccccgccagt gacgataccg cccctgcggt ggcagaagat acggcagctg cgctggagca
    36121 gaaaagcggc tcagcaatga atcagctgtt taacacgctt tcagacggct ggacggtgat
    36181 ttcagacggt ctgcatgatg tacaggccat gaccgacacc atcagcgata agattgatgc
    36241 actggagaat gccgtttcgg ggatggggat tgtgcaggat atcagtgcct ttacggccag
    36301 ttttactgcc ctgaaaggca atgccgccgc cctgattaat gcgccccgtc gcatggctga
    36361 atcgctggcc ggaatgtttg ccgtgctgac cgggctgccc ggcgatcctt ccctgtctct
    36421 gaccggaaaa gccggaacgc cgtccggcag cctgaccaca aaccgtgaca gcatagcaga
    36481 gcaggccatg ccgcagctgt accgcacact ttcctccctg cgttacaccc tcagcgaaca
    36541 ggatgatcca cagcgcctga ttggcctgac gcctgcggca cagaaaaata tccgtctgct
    36601 gcggagcgtg atgcagagtg caaccctggt gtcgcaggca cagacgctcg gaaaactgct
    36661 ggatcaggtc atccggcaga atacgacgca cgttcaggat actgaccgtt cgggcggact
    36721 ggcctggctg gaaagttctg ctgatgtgca gcgtatcaac cgggatttga gcgatgccat
    36781 ggaacaacag gtgctggatc tgtccgcaca gggctatacc gggactgccc ttgcattacg
    36841 tgatgcaggg cttgcgctga cagaagattt aaccacacgg agcgtgcatc ttcccggtgc
    36901 ttttcaggtc atggttcgca ccaccgaacc ggcactggtc acgctgtacc gtgccaccgg
    36961 caacagcagt cgctggcagt attttgtccg cagaaacaat atccccgatc cggtttttgt
    37021 tccgggtggc cgcagcgtgg aggtgatcag tgagcagcag gattgaactg tatatcggtg
    37081 gcagtatttt ttccggctgg ctgacggtca gcgtccgtcg ctcgctggag catctggccg
    37141 ggtcgtttga actggggctg atgctgcccg gtgagcgcat cccctcagcc ctgcgaaccg
    37201 gtcagtccct gacgctcaga attaacgggc agacggtcat cagtggctgg ctggatcagg
    37261 tcagtcagcg gatcagtgca acccgtcatc agatcagcat cagcggtcgg gataaaaccg
    37321 gcgacctggt ggactgtgcc gccatccatc ccggcagcca gtggcgcaac cggacgctgg
    37381 cacagattgc cgcagattta tgtgcgccct tcgggatagc cgtgcgctgg caggttaacg
    37441 acgacacggc tgcacgcccc ttcagctctt ttacgctgga aaactcagaa accgtggcgg
    37501 atgcgctgac ccgtgctgcc cggcatcgcg gggtgctggt gaccagtaat gctgacggcg
    37561 atctggtgtt cacccaggca ggaagccagc agacggacag actggtgctg ggagataacc
    37621 tgcttgatgc cgattacaac acggactggc gaggacgata cagcgaatac cgtgtccggg
    37681 gacacgggcg cggtggtggc aaacggggag acagcgaatc cgccgcccgt ctggcagcac
    37741 ctgtgggcgt catcagtgat gagcagatcg gccgctaccg gccgaaaatc atcctcgccg
    37801 atcaacagac agacaccacc ggtgcacggc agcgtgccct gcgtgaaatg cgccgtgcga
    37861 ttgcccgttc agaacggttt tctgccaccg tgcgtggctg gttccgggat gatggccggt
    37921 tatgggatgt caatctgctg accggtgttt cagccctgcg tttcggtata gaacagactg
    37981 aactgctggt ctgtcaggta gagtttttac ttgatgaaca taacggggaa gtcacccggc
    38041 tggtactggc accgcgtgac ggctttatcg ttccggcaga gccggacagt aagggccggg
    38101 gtggttccgg tgacgatgtt gatgccttta ttcgccagca gatgaaaaaa caggggatca
    38161 gctttgatga atgatgaagt gttcagccgc ctgattgccc cggtaacgcg cggtattcgc
    38221 ctgttgtttg gccggggcgt tctgaccggc acacatgacg aactgaaaat gcagaatgtg
    38281 cagctcaccg gcatggacgg cgaaaccttt gatgatgtgg agcgtcccca gcagtacggg
    38341 cagatcagcg ttcccctgcc gggtgcggaa accttttttg cctgtctggg cggacagcgg
    38401 gatcagactg tggtgcttgt ggtggaagac cggcgcagcc gcccgaccgg actcacagcc
    38461 ggagatacgg gggtgtatca ccatgagggg caccggatac ggttaacaaa ggatggtcgc
    38521 attattgtga catgcaaaat gctggagatt tacgccgatg aggggatgcg ggtggatacg
    38581 ccggaggcca cctttacggg caatgtgacg gtggataaaa acctgcatgt taagggtaat
    38641 ttcgtgcttg atggtacagg taaatcagac gggctgttca cgatgtctga tgccgttatt
    38701 gccgggatca cctattccgg ccatgtgcat caggataacg gcaaaggcag taagacagga
    38761 gcgccggaga atggctgata ttgcaattgt atgggatcag gggtgcggtt cgctgcaact
    38821 gaacggtgcc gacctgctga cggatgacag tctgctgacg gccgttctga tttcgctgtt
    38881 tactgaccgc cgggcgctgg catcggatga aatccctgac ggtacgcgtg accgccgggg
    38941 atggtgggga gacagttttc gcccgcgtcc cattggctcc cgtctgtggc ttctccgccg
    39001 ggaaaaaacg ctggcctccg tgataagccg tgcccgtgcc tatgcggatg aggcgctggg
    39061 ctggctcagt caggatggcg tggcgtcatc cgtggtctgt catgcagaac gtgtggggca
    39121 cgcgcggctg gcgctttcgg tgcgcataac cctgcctgat ggttcagtga ggcccatgat
    39181 tttttatgct gatcttaagg gggagtaatg ccttatcagc ctttaccgct ggcacagtta
    39241 atcacgcaga cacagcagga tatcagccag cgcctgcccg gctcgcagcc gggtgtgaat
    39301 gaaaccaccc tgaatgccat tgcttacgct caggcagggt tatctgctca ggagcatgag
    39361 catctggcct ggattgcgcg tcagatcatc ccgaccgaag ccgatgaagc cgaactgctg
    39421 aaacactgtg cattctgggg cgtcatgcgt aagcctgcct ctcgggggga cgggccggtt
    39481 cagctgatgc tgaccacgga tgcggggatc aaggaaggtg tgcttctcca gcgaagtgat
    39541 ggcgtggttt accgtatcac cacctcactg accggtaagg ccggtacgct gaatgtcagt
    39601 gtggaagccg aaagtgccgg tcgtgcgggg aatgccccgg cagggacaaa actgacattt
    39661 atcacaccgc aggcggggat caaccagacg gccacggtga ccggaacggg gatcaccggt
    39721 ggtgcggatg tggaaaccgt gccggagttg ctttcccgtc tggttttccg ggtgcagaat
    39781 ccaccgtccg gcggcacgca gtatgatttt gaacgctggg cgcgtgaagt gccgggcgtg
    39841 acgcgggcat ggtgccgccc ggaatggccg caggcgggca gcgtgggggt aacgtttgtt
    39901 caggataata acccggatat tttccccggt gacggtgatg ttcagcgggt ggcggattat
    39961 atccgcagtc atgatgatcc ggcgaccggc cagcctgtcg gacagcctct ggggccgacg
    40021 gtgaccgtgt ttaaactgac caataagccg gtgcccttca gcatcaggat catcccgaaa
    40081 acaccggaga atcaggcggc cgtaaaacag gcgctgaccg acctgttata caacgaatcc
    40141 cggcccggcg gtctggttct gccgtcgtct ttctggcgtg ctgtggcggg ggtgaaaaat
    40201 ctggaggatt ttgaagtgcg cagtccgctg acgtcagttc aggcgggaga cagtgagctg
    40261 ctgacggtgg gagaaatcac atggctgtaa ccctgacccc acatcagcgc gccctgttgc
    40321 agttactgcc tgacgggctg gcatgggaca agcgtccgtc atccgtactg gcatcattat
    40381 gtctgggcct cagtcattcg acagcgcgtg tatcctggac gggtaaacag ctgcttgccg
    40441 agcgtttccc tgacacgtcg cgcctgctgc tggaagactg ggagcgttat ctcgggcttc
    40501 ccgagtgtga tatggccgga gccaccctca cggagcgaca gcgttatgcc ggaaataaat
    40561 accggatgaa gccctcgctg aaccgtgaat tttatatccg gtttgcggcg gagtttggtt
    40621 atcagattga cattcagccc tcaccggatt cgctgtgggt cagtattgtc acgattaaca
    40681 gtgaaaccgg ctaccggaat atgaatgtgc tggatgatat tctcacgccg ctgcgtattt
    40741 atgaaggcgg tgcgctggaa tgtattctga accgttataa gcctgcatgg cagacgttta
    40801 tttacgttta tgcaaacagc catgaagagg agactattta atgtttcatg ttgataataa
    40861 ttccggcgtg gcgaatatgc ctgcgctggc accggcgcag agtaatacca caacctggtt
    40921 taccgaaggt gacggacaaa aaggtatcag ctggattggt caggactggc tgaatattct
    40981 ccaggccgaa ctgctgaata ttctggctga agccagtatt cagccggata aggcgcagtt
    41041 aaaccagctt acgctgtcca ttaaagccat tatcgctgcg aatgcctttt cccggaaaaa
    41101 taacctgaaa gaaattgctg atgccggtgc ggaggcccag cgtctggccc gtggttatct
    41161 tggtctgggg acgcttgcca caaaaaacag tcttggtccc ggtgacgtta atgccctggc
    41221 gaaggatcag aatctcgccg acctggagaa taagggaacc gcccgtaata atctggatgt
    41281 ttacagcaaa agcgaaggtg ataaccgtta cctgcgcagg gagcagaacg gcgcagacat
    41341 tccggataaa ggggctttta tcgataacgt cggtttacgg gaaacggtga ataaggcggc
    41401 gaatgctctg ccatcggacg gcaccgccgt tgccgcgaat aaactctcca ccccaagaaa
    41461 tattaatggc gttccttttg acgggacgca ggacatatac attacctccg gtatgactga
    41521 agctactgct gataacaaat acgttagcaa cgttcagctt ggtgcccaga gttaccattc
    41581 acccggaggt aatgaaatgt cctggagtta tggtgcacct tctggttgta tgctttcagg
    41641 tattaacgtg caggataccg ggaaaagttc agccgataat atcggaggcg tttattaccg
    41701 tccggtgcag atatatatcg gtaatgcctg gcgcacagtt tcatccgtct gattaaagga
    41761 aaagggtgcg ataagcaccc tttatgttat tcaggcttct ccggccattt aatggcgtca
    41821 aattcagctt tgtttttaat cgctggtagt tccatttttt tcacctgact gatgtatttc
    41881 atccatcgtg taagcgcagc tctgttttca tcactaatcg tgcccagctg taactcagtt
    41941 ctccagtcat taatcttttc atatgcctga ttaagatatg actggcgcag tgcttccgct
    42001 tttttggtat agttaaccgg gagttgtgag atgacaccat tatcaaactt ccagttacct
    42061 gaaatatcag taccttcagg taattcgtct gtctcaacaa cggaaaaacc cgcaggatac
    42121 agcgccgaag catcctgtga tattgagcag ataacaccgc tgtcaggggt tatgcaaagt
    42181 ttatattttt tggtgaataa tggcagggac tcatagaaat ccttaccatc ttcactttga
    42241 aaatactgta catcattacc aaagggtttt tgttccgggt aatatcgttt aacattaatc
    42301 agttgcataa acatcaccat gtaaatcaat gataaaaaca ggaataataa tatcgccata
    42361 tacaggcaag gggtttctga aataacgcat attttcagat gctgaccgca gcgtcatgaa
    42421 tcgttaaatc aattataagc cggtaatatg cggttatcgt attggcctgt ccatcctttg
    42481 tcgttatgtt cctgcctaac cggctccgtc acaaaaagta ccgtgatgct ttttttgaat
    42541 ttccgcaaaa cacatattta tcagcggttg ttattctgac tctgtttaat tcagaaacag
    42601 gcagaaaaga cgatgtcaga ctccaagtgg aaaatgctct ccgccatgcc cggagagttt
    42661 tcagtcaagg ttgccggtgg cacggtggca tttattgaaa gccctttccg cccttcgggt
    42721 aataaagggg ggatcacctt cgcagactgc gtgatccgct tcagcacgaa agagccatta
    42781 tgggtaatgc cggtatccgg taatcccagt gcagaaatca ccagttcagg cgtgactggc
    42841 atcattccaa tcacatctga cgtggccgga acgctcacgc cctccgactg gaatgcaccg
    42901 gataacgccg ggccttccgg tagcacgaat ggcagcagtt cagagccgga atattacttt
    42961 gttgtcattc tcgcgggtca gtcaaatggc atggcctacg gcgaggggct gccgttaccg
    43021 gattcgtttg accgcccgga cccacgcatt aagcaactgg cacgccgcag tacggtaacg
    43081 cccaacggta cgtcgtgtac atacaacgac atcattccgg cagaccactg cctgcatgat
    43141 gttcaggata tgagcgggat aaaccacccg aaagctgatc tggcaaaagg ccagtacggg
    43201 actgtcagtc aggggttgca tattgccaaa aaactgcttc cctatatccc acagaatgcg
    43261 gggatacttc tggtgccctg ttgtcgtggc gggtcagcat tcaccactgg agccgacggc
    43321 tcattcagtg aggccagcgg tgcttccgct gattcttcac gctggggggc cggtaaacct
    43381 ctgtatcagg acctggtaag ccgcaccaaa gtcgcgctgg cgaaaaaccc gaaaaacaaa
    43441 cttctggctg tggtctggat gcagggagag gcagaccttg cctcaggaag ccagcagcat
    43501 aatagtttat tcacggccat ggttcagcag ttcagaactg acctggctcc gcttgctgcg
    43561 cagtgcgtga gtggaaatgc caccacggtg ccgtggattt gcggtgatac cacgtattac
    43621 tggaaaaacg ctggcaccga taaatatgag gcagtatacg gtggctacaa aggcaaggaa
    43681 gcacagaaaa ttttctttgt accgttcctg acggatgaga atggacagag cacgccaacg
    43741 aatgctccgg cggaagaccc ggatattgtg gctgtcgggt attacggtgc agcatcccga
    43801 acccagggca gttttgtttc gacacagcgt gacagccatt tcagctcatg ggcacgcagg
    43861 ggcgtcattt cagaccgtct ggcctcagct attatgctcc atgcagggcg cacggctgaa
    43921 ttgatgcgcg ggcagaccgt gacaccaccg gatgagaagc cgtcacctga tacaccatca
    43981 aaaccgtcca cgccacccgc agacaccacg acgatgagta cgctatttgc ttaccgggca
    44041 tctgagtctg aaggacggtt gacaccacag ggttgggctg ccggaggtgg caaggctcaa
    44101 atcgtggatg atgccggagc cagcgggggt aaggcaatga aactgaccaa ggaaacagga
    44161 agagcctcct ggtaccttga gcatgatgct ggtaatggcg cagacctcct gggtaaaggc
    44221 ggtcttgtca gttgtcgttt taaacttgat ggtgcgctta cggctaatcg gtatgcactg
    44281 gcgctatact ggccggtttc ttcactgcct cagggtgtca tgctggaagg taattcaggt
    44341 cataacttgc tggcgtcatt ctatgtacag agcgatgcca cagaccttaa cgtgatgtac
    44401 cacaagggca atacggcgca aaacacgaaa ctggggtcat tcggtgcatt taataacgaa
    44461 tggcatacgc tgggcttccg ttttgccggt aacaacagta ttgaggtgac gccggtcatt
    44521 gatggtaagg acgggacacc attcatgctg tcgcagtctc cggtcggcac gtttacggca
    44581 gacaagttgc gcgtgaccga tatcactaaa aatgcgactt acccggtact gattgacagt
    44641 atcgtggtgg aagtgaataa cgcgtaagca ggataaaaaa atgccgccgg taagggaaaa
    44701 caagaaaccg gcggcaaaaa gatcgttata tccaaagcaa aaaatgcagt gcaccttttt
    44761 acctaacaga tattaccggt gtcaacacca tgtcaataat tgggagggat aatgacattt
    44821 ttacagctta ttttattgta tttctgcacg gcagtatgtg cgttatatct ggtgagtggt
    44881 ggttataagg ccatcagaaa ttatgttcgc cgcaaaattg aggacgcagc agctgaaaaa
    44941 ctcagtaaaa cagcgcaggt aactccgctc ccaaaagacc cgacccagcc ctgattgcag
    45001 ggctttattc ccgcataagg aggatataca tgccagaaat taaaggcacc gtcactgaag
    45061 acctggtaaa acaggcactg tattccggtg aagtaaataa gttgctgaaa gcgcaggtcc
    45121 gcaaggattt tgaggcacaa atcgacactt acgttgatga agtgctggca aaacttattg
    45181 ggccagcagt tgcggccagt gatgctgata atgagccgaa aaccgacgcg cagccagagc
    45241 aaccagaccc ggctcagccc ggaactgaca gtaccatgat gtaacaatac aaaccggtgg
    45301 tgtcctctgc cggtttgaca ctaatgaaaa catctttgcc gctatccagc catatgtaat
    45361 cgctggatag tatttcgtat tcagaaatat agctgcttat ggagcagtca atcctgcttc
    45421 cccatccggg caggcaaatg aaccggtatc attagtccga ttcacgcttt tgtagaatcg
    45481 ttcccagaat gctatacagc tcattagcct gttgctccat cattgagacg gaggctatac
    45541 ggtgcaaagt gatttcagga ggtggcacac ctccatcttc ttgctgtgga ggaaaaacat
    45601 aagatttaat aaaaaagttg aagcatatga actctcccgc actcgaactg tatttgcagg
    45661 catctgaata atcggaaaaa taatcattat agatttccgc attttcaatc atttttcgat
    45721 ttctcgtcgt tacatgattg tccatagcag cctcttacgc tggcaattaa ccaattgact
    45781 tctataaatt agttgatttg tttgctttgc ggtattcaaa tgtacaatca ttcagcactt
    45841 atgcgttaca gcaccgttta aaattagtga tcaaactcat aaattttcgc taatgacatt
    45901 tcgtgttatt tgttgtatca attaaataaa tcaaatcgat cgtttttgtc gatcgattga
    45961 gtgataaaga tatcaacaac aaagcaggag gttatgcggc catgtatcga aacatacggt
    46021 gccgacattg taacaagcta ctggcacgag ccagcttcag ttatctggaa gttaaatgtc
    46081 cgcgctgtaa aaccctcaat caaatcacat ctcagagcgc cacagagcac cccacataca
    46141 caaggaaatc ctgtcgtggg gaacagaaag caagttacat cccgtatcat cagcacgcct
    46201 gaacttattc actataacga caatatcgtc ggttatggct cccgtgagtt acgggttgag
    46261 acaataagtt gctggctggc ccggctggtc gtcgtcaata aacattacag ccatcgtttc
    46321 gtaaataatt cctaccttca tctgggcata ttttcagaac gggagctggt tggcgtaatg
    46381 caatggggtt atgcccttaa tccaaacaat ggtgcacgcg tcgtaactgg cacgcagaac
    46441 cgcgaatata tggagcttaa ccggttatgg ctgcatgact gtatgccgag aaactctgaa
    46501 tcaagggcaa tcagttacgc gctcaaacta atcagacaac tttatccgca ggtgcagtgg
    46561 gttcagtcgt ttgcagatga gcgttgtggc tgtcttggtg tcgtgtacca ggcaagcaat
    46621 tttgattatg taggcagtca tgaaacaaca ttctatgaac ttgatggcga gtggtatcac
    46681 gagatttgca agaatgcaat caaacgagga ggacagcgag gtgaacatct gcgggctaat
    46741 atcgacagag ccagtgttca taaattccgt cagtttcgtt acatccggtt tctggacaag
    46801 agagccagaa aacgccttaa cacaaagcta ttcaaagtcc agccttaccc aaaaccacaa
    46861 acagttaaaa ctggtttgaa agagagtgaa tgaacgctta aaaactcatt ctaaaactca
    46921 aaagaaaagc gccgatttag tatcaaataa gttgcgtatc ggcgtttttc agcggtatca
    46981 aatgaaatgc aaatcggtat caaaaaaatc gccgcgctat acacgttcca cgtttacccg
    47041 cgctataatc taccggccct tcctgataat aggccagagc aattctccac ggcccgttgg
    47101 cggcattaaa atagactgaa ggctccgcc
//
LOCUS       DAKISA010000023        46162 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.023, whole genome shotgun sequence.
ACCESSION   DAKISA010000023 DAKISA010000000
VERSION     DAKISA010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46162
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.023"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..716)
                     /locus_tag="OVW45_001328"
     CDS             complement(<1..716)
                     /locus_tag="OVW45_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCV5525828.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSSA"
     gene            complement(730..1389)
                     /gene="pgmB"
                     /locus_tag="OVW45_001329"
     CDS             complement(730..1389)
                     /gene="pgmB"
                     /locus_tag="OVW45_001329"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCV5525829.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLL
                     ADLRAQQIPVGLASVSLNAPTILDALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGITGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(1386..3653)
                     /gene="ycjT"
                     /locus_tag="OVW45_001330"
     CDS             complement(1386..3653)
                     /gene="ycjT"
                     /locus_tag="OVW45_001330"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCV5525830.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTAATHQEDE"
     gene            complement(3650..4705)
                     /locus_tag="OVW45_001331"
     CDS             complement(3650..4705)
                     /locus_tag="OVW45_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCV5525831.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPLAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRKMADDNRHLRSMEAFINHVQGKPVMIADAKQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(4715..5503)
                     /locus_tag="OVW45_001332"
     CDS             complement(4715..5503)
                     /locus_tag="OVW45_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCV5525832.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGDKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(5521..6573)
                     /locus_tag="OVW45_001333"
     CDS             complement(5521..6573)
                     /locus_tag="OVW45_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCV5525833.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKVRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSAFRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(6604..7446)
                     /locus_tag="OVW45_001334"
     CDS             complement(6604..7446)
                     /locus_tag="OVW45_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCV5525834.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSMVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSAGNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(7433..8314)
                     /locus_tag="OVW45_001335"
     CDS             complement(7433..8314)
                     /locus_tag="OVW45_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCV5525835.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(8335..9645)
                     /locus_tag="OVW45_001336"
     CDS             complement(8335..9645)
                     /locus_tag="OVW45_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCV5525836.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKTVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDMPGSNDIMEVKDAFMNGTAPMAIYSTYIL
                     PAVIKEGDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKAEETEAAEKFVTFMEQADNI
                     ADWVMMSPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNF
                     TRMGDVTGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(9659..11190)
                     /locus_tag="OVW45_001337"
                     /pseudo
     CDS             complement(9659..11190)
                     /locus_tag="OVW45_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
     gene            complement(11402..11716)
                     /gene="pspE"
                     /locus_tag="OVW45_001338"
     CDS             complement(11402..11716)
                     /gene="pspE"
                     /locus_tag="OVW45_001338"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCV5525837.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(11791..12012)
                     /gene="pspD"
                     /locus_tag="OVW45_001339"
     CDS             complement(11791..12012)
                     /gene="pspD"
                     /locus_tag="OVW45_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCV5525838.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(12021..12380)
                     /gene="pspC"
                     /locus_tag="OVW45_001340"
     CDS             complement(12021..12380)
                     /gene="pspC"
                     /locus_tag="OVW45_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCV5525839.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(12380..12604)
                     /gene="pspB"
                     /locus_tag="OVW45_001341"
     CDS             complement(12380..12604)
                     /gene="pspB"
                     /locus_tag="OVW45_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCV5525840.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRDELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(12658..13326)
                     /gene="pspA"
                     /locus_tag="OVW45_001342"
     CDS             complement(12658..13326)
                     /gene="pspA"
                     /locus_tag="OVW45_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCV5525841.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            13493..14470
                     /gene="pspF"
                     /locus_tag="OVW45_001343"
     CDS             13493..14470
                     /gene="pspF"
                     /locus_tag="OVW45_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCV5525842.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNAVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQKGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(14588..15853)
                     /gene="puuE"
                     /locus_tag="OVW45_001344"
                     /pseudo
     CDS             complement(14588..15853)
                     /gene="puuE"
                     /locus_tag="OVW45_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
     gene            complement(15891..17171)
                     /gene="puuB"
                     /locus_tag="OVW45_001345"
     CDS             complement(15891..17171)
                     /gene="puuB"
                     /locus_tag="OVW45_001345"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCV5525843.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPYYPFPGGRTLRVPFTAMGAAWYSLRDRLGV"
     gene            complement(17173..18663)
                     /gene="puuC"
                     /locus_tag="OVW45_001346"
     CDS             complement(17173..18663)
                     /gene="puuC"
                     /locus_tag="OVW45_001346"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HCV5525844.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDHAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEEAGLPDGVL
                     NVVSGYGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANI
                     VFADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGH
                     PLDTATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNA
                     SLSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSV
                     FVNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(18813..19370)
                     /gene="puuR"
                     /locus_tag="OVW45_001347"
     CDS             complement(18813..19370)
                     /gene="puuR"
                     /locus_tag="OVW45_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCV5525845.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(19397..20161)
                     /gene="puuD"
                     /locus_tag="OVW45_001348"
     CDS             complement(19397..20161)
                     /gene="puuD"
                     /locus_tag="OVW45_001348"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCV5525846.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            20373..21734
                     /gene="puuA"
                     /locus_tag="OVW45_001349"
     CDS             20373..21734
                     /gene="puuA"
                     /locus_tag="OVW45_001349"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCV5525847.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRDAEGNLQPPC
                     APGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYH
                     TDNVLEACDDALALKRLVRLMAGKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENV
                     LSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNRT
                     VALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGLEQ
                     EGLPFPIRQSDALGEFIENDHLRRYLGKRFCHVYHACKNDELLQFERLITETEIEWML
                     KNA"
     gene            21883..22047
                     /locus_tag="OVW45_001350"
     CDS             21883..22047
                     /locus_tag="OVW45_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525848.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            22037..23422
                     /gene="puuP"
                     /locus_tag="OVW45_001351"
     CDS             22037..23422
                     /gene="puuP"
                     /locus_tag="OVW45_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCV5525849.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            23556..23801
                     /locus_tag="OVW45_001352"
     CDS             23556..23801
                     /locus_tag="OVW45_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCV5525850.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            24114..25757
                     /gene="sapA"
                     /locus_tag="OVW45_001353"
     CDS             24114..25757
                     /gene="sapA"
                     /locus_tag="OVW45_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCV5525851.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            25754..26719
                     /gene="sapB"
                     /locus_tag="OVW45_001354"
     CDS             25754..26719
                     /gene="sapB"
                     /locus_tag="OVW45_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCV5525852.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILTFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            26706..27596
                     /gene="sapC"
                     /locus_tag="OVW45_001355"
     CDS             26706..27596
                     /gene="sapC"
                     /locus_tag="OVW45_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCV5525853.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            27596..28588
                     /gene="sapD"
                     /locus_tag="OVW45_001356"
     CDS             27596..28588
                     /gene="sapD"
                     /locus_tag="OVW45_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCV5525854.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            28590..29396
                     /gene="sapF"
                     /locus_tag="OVW45_001357"
     CDS             28590..29396
                     /gene="sapF"
                     /locus_tag="OVW45_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCV5525855.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            29464..29817
                     /locus_tag="OVW45_001358"
     CDS             29464..29817
                     /locus_tag="OVW45_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCV5525856.1"
                     /translation="MMDKIKSNARDLRRNLTLQEQKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     PEIILQELNRRSPSP"
     gene            30185..30973
                     /gene="fabI"
                     /locus_tag="OVW45_001359"
     CDS             30185..30973
                     /gene="fabI"
                     /locus_tag="OVW45_001359"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCV5525857.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            31118..32244
                     /locus_tag="OVW45_001360"
                     /pseudo
     CDS             31118..32244
                     /locus_tag="OVW45_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
     gene            32312..34246
                     /gene="rnb"
                     /locus_tag="OVW45_001361"
     CDS             32312..34246
                     /gene="rnb"
                     /locus_tag="OVW45_001361"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCV5525858.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRGIIARPVA"
     gene            complement(34324..34427)
                     /locus_tag="OVW45_001362"
                     /pseudo
     CDS             complement(34324..34427)
                     /locus_tag="OVW45_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            34481..36471
                     /gene="pdeR"
                     /locus_tag="OVW45_001363"
                     /pseudo
     CDS             34481..36471
                     /gene="pdeR"
                     /locus_tag="OVW45_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            36618..36791
                     /locus_tag="OVW45_001364"
     CDS             36618..36791
                     /locus_tag="OVW45_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525859.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            36881..37630
                     /locus_tag="OVW45_001365"
     CDS             36881..37630
                     /locus_tag="OVW45_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCV5525860.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            37899..38117
                     /gene="osmB"
                     /locus_tag="OVW45_001366"
     CDS             37899..38117
                     /gene="osmB"
                     /locus_tag="OVW45_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCV5525861.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(38243..38569)
                     /gene="yciH"
                     /locus_tag="OVW45_001367"
     CDS             complement(38243..38569)
                     /gene="yciH"
                     /locus_tag="OVW45_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCV5525862.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(38569..39306)
                     /gene="pyrF"
                     /locus_tag="OVW45_001368"
     CDS             complement(38569..39306)
                     /gene="pyrF"
                     /locus_tag="OVW45_001368"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCV5525863.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(39499..40668)
                     /gene="lapB"
                     /locus_tag="OVW45_001369"
     CDS             complement(39499..40668)
                     /gene="lapB"
                     /locus_tag="OVW45_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCV5525864.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(40675..40983)
                     /gene="lapA"
                     /locus_tag="OVW45_001370"
     CDS             complement(40675..40983)
                     /gene="lapA"
                     /locus_tag="OVW45_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCV5525865.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGVAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(41132..41896)
                     /gene="pgpB"
                     /locus_tag="OVW45_001371"
     CDS             complement(41132..41896)
                     /gene="pgpB"
                     /locus_tag="OVW45_001371"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCV5525866.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAVIIVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            42066..42656
                     /gene="ribA"
                     /locus_tag="OVW45_001372"
     CDS             42066..42656
                     /gene="ribA"
                     /locus_tag="OVW45_001372"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCV5525867.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(42720..45395)
                     /gene="acnA"
                     /locus_tag="OVW45_001373"
     CDS             complement(42720..45395)
                     /gene="acnA"
                     /locus_tag="OVW45_001373"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCV5525868.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVEKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKV
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVILGLKRQPWVKASLAPGSKVVSDYLAKAKLTPFLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(45559..45654)
                     /locus_tag="OVW45_001374"
     CDS             complement(45559..45654)
                     /locus_tag="OVW45_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525869.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(45768..45935)
                     /locus_tag="OVW45_001375"
     CDS             complement(45768..45935)
                     /locus_tag="OVW45_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525870.1"
                     /translation="MVGQEQLKSSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(45938..46102)
                     /locus_tag="OVW45_001376"
     CDS             complement(45938..46102)
                     /locus_tag="OVW45_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCV5525871.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
BASE COUNT        10920 a        12206 c        11629 g        11407 t
ORIGIN      
        1 gccgatgatc caataaatcc ggcaacaaac atattcgctg gttggttata aaccgttttc
       61 ggcgcaccaa cttgctgaac aatcccatcc ttcataatca caatccgcgt cgccatggtc
      121 atcgcttcgg tctgatcgtg ggtcacgtag atcatggtgg tattcagctt ctggtgcagc
      181 ttgctgatct ctgcgcgcat ttgcacgcgc agcttggcat caaggttaga gagcggttca
      241 tccattaaaa acacgcccgc ttcgcgcaca atcgcccgcc cgagcgccac acgctggcgt
      301 tgcccgccgg aaagcgcgcc cggcttgcgt ttcaggtact cacgcaggcc gagaatttgc
      361 gccgcccagt tcacccgctc atcaatcacc tctttggcga ttttttgcat cttcaggcca
      421 aacgccatgt tgtcgtaaac cgtcatatgc ggatacaacg cgtagttctg gaacaccatc
      481 gctatattgc gtgctttggc tggaacgtca ttcattcgtt tgccgtcgat caacagatcg
      541 ccgccgctga tctcctcaag cccggcaatc atgcgcaggg tggtcgactt accgcagcct
      601 gacggaccga caaacacgat gaactcttta tcggcaattt ccaggttgaa gtccttcacc
      661 acatgcacct ggttatcgta gattttttga atatgttgta acgaaagctg agccatgttg
      721 attcctttgc tatacgtttt gccagaaggc cgataaccgc gaccaggtca gggagtccgt
      781 tgaaggcaat agtaattgcg ccccggttat gcccgcgccg atccccaccg agcgcatacc
      841 gctggcgtta atggcgtcaa tgcccgcctg cgcatcttca atgccgatac atgcctgcgg
      901 cggcacgccc agcccggcac aggcagcgag aaagatttcc ggatccggtt tcgagttttt
      961 aagttgggaa gcatccgcgc agaaggtgaa aaactcacgc agctccagtg catctaaaat
     1021 cgtcggcgca ttgagggaga cagaagccag ccctaccggg atctgttgtg cgcggagatc
     1081 tgccagcaaa gagcgaatgc cgggtagaac agcgttgacc gtcaactcgc gcaatgagtg
     1141 gacatagagc agatttttgc gatacgccag ttgcgccctc tcctgcgagt taaagtcgcc
     1201 ctctttgccc ccgtgttgca gaatgcgccg cagagactca tcgcggctga tcccttttag
     1261 ggattcgtta aactgcgcat caatgctgat gccaatttca gcggcaatct gctgccacgc
     1321 ctggaaatgc agatgcgcgg tatcggtgat tacaccatcc agatcgaaaa ttaccccttg
     1381 cagtttcatt catcctcctg atgtgtggca gcggtcccat tgaagggcaa aataaaatca
     1441 cccaaacaga aaacagattc ttcagccacg gatataagct gaccgttcaa acgcagtgaa
     1501 acgggggcag aagttcgaat cgcaatacgc tgcgcgtcaa gagtgacctg taattcgcag
     1561 ccctgccaga acagagggaa agacaactgt tgccactgct caggtaacgc cggattgaga
     1621 tgcaattcac cgtcacgcac gctcaccccg gcaaaaccct gaatcgcccc cagccagata
     1681 gcgccagttg cggcagcatg gataccatca tcacaactat gcggatcagc accaagatcg
     1741 atttcagtcc cctcgcgcca gaactgataa ctttgggtca gcaggccaca gcgtgcggca
     1801 acaatgccgt ggattgcttt acttaatgac gagtcgtgaa tagtgcgcgg ttcataaaat
     1861 tgcagattgg caagacacga tgccgctgag aactgctccg gcagcatgta attgagcatc
     1921 accacatcag cttgtttgag gatttgcatc tcgttcactt ctgcgcgtga ataatccagc
     1981 agaatggttt gcttccccgc cgccgctttg tatttcgcaa gattaatcgc cggcttagcc
     2041 ataaacgaat catcctgcgg caaaacgccg tcgggctgaa tttctggcag ccgcagttct
     2101 ttaaggaaca tttcggcacg atgaataaac gcgtcgtcgc tacagccaaa ctggcgggca
     2161 atactcagcg cctgctggac gttgtaatac gccatatagc tggtgaaggc gttattattg
     2221 acatgttcgg tatattcgtc aggcccaata acatcatgaa tttccagacg gtcgttaacc
     2281 ctcaccgcgc ggctaatcca gaactttgca gtttccagaa gtagcgccat gccttcatgc
     2341 gcaatgaaac tttcatcccc cgtggtctgc cagtattgaa taaccgccca ggcgatatcg
     2401 gccaccagat gatgttccgc ctgcgccgag gccacttttt gccgcagccc ggtgcgaatg
     2461 ttaatggcgg caaattccgg cgtctcttct tcgccgctgc gcgcgctttc ccacggaaat
     2521 agcgcgccct gccagccgtt gcgtcgcgct ttctcctgcg cgcctggcaa gttgtgccag
     2581 cgataacgca gtaaacttcg ggcaaccgtc ggatcgctaa acagatgaaa cggtaacaaa
     2641 aatacttctg tatcccagaa aacgtggcct ttgtagcctt cgccggttaa gccttttgcc
     2701 gcaatgctgc tgcgctcgtc gtgggcaggc gtcatgatgc gcagatgata aagcgcataa
     2761 tctaacgctt gctgatcgtg cgcttcgccg ccatttaccg tgatacgacg tttctgccac
     2821 cattgacgcc agttttctgt tgatactgca agaagttggt cgtaactctg ctgcgcgcac
     2881 atttcaagct ggcgaagcga cgcgctgccc cactcgtcta aaacggcttg cctgtcatcc
     2941 cgccagtcga tccacaccag tttttgcaac gtcactgtct cgcctgcatg aagctgcgca
     3001 ctggtatgtt gcagtaaacg gcgctctttg gcggtatagc attgctgcac atcaccgctc
     3061 accttacagc aacagctgat ggccacatca ctgcgtccat cctgggtggt gtagatcccc
     3121 tgcatcaaat gctgaccaaa cacccgcacc tgggtttcgt cgagatgctg gcgaccgtgg
     3181 ttggtctgcg tggcgtcaat gcctgttgaa atcagcactg acgcgtcggc gtccagtggc
     3241 gtaatagtga tttccagcac aatgagcggc agttggtctg ccgaaacaaa gcgacggctg
     3301 gcgatggtgt aacctgtgcc gttgctggta cgccagacaa cgctacggcg taattctcca
     3361 ctggcaaagt caagttcacg ctgccaggct tcgcgggata acgagaaaac ctcaccattt
     3421 atggcaatct ccatccccac gacatcaggc aggttcacca gttcgttgat ttcacctttt
     3481 cccgcccgat gatacagccc cgccagatac atccctcgcg tctggcgcgt gtaatcttct
     3541 tcatggctgg cgcgaagccc aagatagccg ttcccctgcg ccatcagcga tgcatacttg
     3601 ttcagggtat gctggctgaa atggggttct gataacgtta ctggcctggt cataattcca
     3661 cacgcgtccc tgtttctgcg gattgataca acgccgccac cagttgctgg atgatgtacc
     3721 cctgcttggc gtcggctatc atcacgggct tgccctgtac gtgattgata aaggcttcca
     3781 tgctgcgcaa atggcggttg tcgtctgcca ttttccgttg catcagcgtc attaattcac
     3841 cgttgttatc ggtgtagata tgtgctggaa acagcgtcgc accagcttta tcaccacaaa
     3901 agctgacgtt cataatcgac tgttcgcgga tgttgagtgc aaatgacgtt tccagccaca
     3961 gaatgccgcc gttatgaaat tcaatggtgc caaacagcga atcttcgacg ctgtaagttg
     4021 ccggatccca ctcaccaaat tgaccacagc tcttttgcgt gccgatcttt tgaaagctat
     4081 gcgcattcac gcttttcacc gccggaaagc ccagcacata catcgcagca tccagcatat
     4141 gaatgccgat gtcgatcagc gggccaccac cctgcagttc tttattggta aagacacccc
     4201 agccgggaac gccgcagcga cgcagggcgc gggcggtggt aacgtaaatc tctcccaaaa
     4261 cgccgttggt cacctgttca cgcagctgtt gcgtatcgag cgcaaaacga tggtgaaagt
     4321 cgtaggccag caccttgccc agtttgcgcg cggtatcgca catttcccgc gcctgttctg
     4381 gcgtcatggc gagcggtttt tcgcacatca catggcagcc cgcttccagt gccatcaggg
     4441 tatgttcgta atgaaaacgg ttaggtgagc agacgctaac cacatcaggt ttcaccgcca
     4501 gcagcatggc ctgcggatcg tcccacacgg atgcattccc gtatttttct gccagcgcct
     4561 gcgcctggga aaggcggctg tcacagacag ccaccagttc cagatcgttg cgggtgcagt
     4621 agtacgaagc atgaacctta tccgccacct ggcctgcgcc tattatcgcg acccgcagcg
     4681 gagagcttgt cattgcactt ttcacaaaga cctcttagca ggtacgcaac caggccaacg
     4741 aatcacggta cgcctgggca ggatcttccg cccggatacg cccttcgtac actacatagc
     4801 cctgatagtt atccgcgcgc agctgttcaa acagcgcgtg gaaatccagg gtgccgctgc
     4861 ccggctggta gcgatggtta tccgcaatat gcacatgacc gagcaggtcg cggttgtcat
     4921 gcagcgcctg cgccaggtta tcttcttcga tattcatgtg atagaaatcg ccgataatct
     4981 gtacatgttt aagatcgttt tcgacgatgt aacggcgggc atcggcgagg gtgttgatca
     5041 tatgatcctg atagcggttt aacggttcga gatacaccac tgttccggta cgcgcggcga
     5101 cctgttccag tacgcgcagg gaatcactca ccattttgcg gtcgccgtcc aggctacgcg
     5161 gcgaggtcat cggcggtaag cggaaggtaa acatgcccca cgctgccgga acgacgatac
     5221 ctttatcccc cacttctgcc agcgcttcga gaatgcgttc gatctgcttt aagccattaa
     5281 gacgacgctc ttcgataaag tcgccaatcc aaccgtcata gccaccgcag gcggtggtca
     5341 ccggtaaacc ggtttctttg attgctgctt tgacttcttc gagattgttg accagcagtt
     5401 tgccgtcaat ctcaaaacca tcgaagccca tctctttgat ataacgaaat ttctccagaa
     5461 tgttttccgg aaagaatgcc tgattctgtg tgcctatttt cattttttca ttcctttgaa
     5521 ttaaaacgta acgcccattt tgatgctctg ttccggatgc tgatcgacat actgcatgta
     5581 gctttccggg ctattggcaa aggtcactac cgggtcgatt aaatcttcgc aattgagata
     5641 accgttcatc agcagttccc agcaggtttc ttcaatacgc ttacggctcc agcgcggata
     5701 atccgggttc ggttcgctgc acgcgcggga gaaaacaatt ttggcgttat tgaaatgcgc
     5761 ttcgcgtccg aggttaaaac cttcagcaaa tggtttggca aacgcaacat aggagatggt
     5821 gccaccgtag gccagaccgc ggaacgccga ttgcagcgcg tcggcatagc cgctggtttc
     5881 gataatcaca tcggcaccct gcttgccggt cagcgttttg atctctttgc ctacgtcagt
     5941 accaatgggg ttaaggcaga aatccgcgcc gtggcgacgg gcaatatcac agcgatgagc
     6001 gataggatcg acgccaatca cgaccgacgc gccagcacgt ttagccagtt gaatggcaat
     6061 ttgaccaatc gcgccaagcc ctaccaccac aacaaaatca cccacgcgca cgttggcatc
     6121 gcgcacgccg ctcatggcaa actgcgccgg gtcgtaacaa acggcgtttt tccacgagct
     6181 gccttccggc attttgcgca gcttgtagtt attcaccgcg ttaatgatga ccgtctcgga
     6241 gagcgggccg tagccgcata cgctgtcgcc caccgcgtag tcggtaacgt cgctgccgca
     6301 ctcgataatg tcgccaacca ccatgtttcc aagctggaat ttgccaaact caatgccgcg
     6361 cggcgcatct gccggacgcg gagtgaacat ctgccattcg ccgttaaagt cttcatcgat
     6421 aaacgggctg gcggcgcgga agtcgaccac ttccgttccg tgtttcggtg cgccgaaacg
     6481 cacgcggacc ttcacttcat tagctaaaat cgcccgatct tcatactcaa ccagcgcagc
     6541 aacacgcggt gctgtggcta ctaacttttt catttaaaac tccttgtaaa gaaactggcc
     6601 gctttagccc ttcacgccac cggcggtcaa accactctta ataaaacgtt ccgaaagcgc
     6661 atacataatc accaccggca atgcggtcac cagtgaggcc gccatcatcc gtccccagat
     6721 gtaatctggc gtactgaaca gcgcgttcag gcccaccggt aaggtgaaat tgccggcgct
     6781 ggagaggaaa atcgacgcaa acagatagtc gttccacgcc accataaagc agtagacgaa
     6841 cacggatatc agcccggaca tcgccaacgg cacggtgatg cggaagataa tttgcagacg
     6901 gttgaggcca tccatcatcg ccgcttcttc gatctcatcg gggatggtgt cgaagtagct
     6961 tttcagcata aacacggcgg tgggcagagt ttgcgtcacc atggtgatga tcagcgccat
     7021 ctcagtgtcg taaatgccca gcgcggtgat gattttgaac agcggcacca ccagcaaaat
     7081 gccagagaac atatacaccg tgtaaaagct ggcgttgatg gtcatccgac ctttaaagcg
     7141 caggcgggaa agcgcgtaag ccccaagaat gccgaggaat accgccacca tggatgaaac
     7201 cacagacacc accagactgt tacggaagta gtcgacaaac gggaaaatca tcgggttaaa
     7261 aatgtcgacg taatgctcca gcgtccactg ctgcggcagc agcgtaggat gcagtgagat
     7321 cgcctctttc gcgcccttga acgaggtcat cagcatcaca aaaaatggaa acagcgtgat
     7381 gatgagaaac agcgccagcc cgcagtaaaa accgatgcga ctgagtgtgc gtttatttgt
     7441 tgccattgag gttcacccgt ttcctggtca gcagaatgac agcgaaaata atgatgaaga
     7501 gcaccaccga aatcgccgcc gctttcccta agtcattaaa ggcaaaggcg gttttgtaga
     7561 gatagacgcc gagaatatcg accttggtgg tcagcagata aacatcagca aacatgtaga
     7621 acatccagat agtgcgcagg gtcaccaccg tcgccaggac cggcataatt gcgggcagcg
     7681 tgacgatgcg aaaccgctgc caggcattag cgccatccat ctctgccgct tcgtacagcg
     7741 atttgtcgat ggtctgcaaa atcgccagaa acgagataaa ggcatacggg aagtagcgcc
     7801 agatggcgaa cagcaccacc agcacaaagc tactgcccgg attatcgaac cacagcggtg
     7861 cctgctcata gagatgcaga agatcgacgc cgaggtagtt cacaatgccg tagccgttgt
     7921 tgaacatgta tttccaggca aacaccagcg aaatggacgg cgttacgtag gagaggatca
     7981 ccagcgaacg cgcggttttg cgcaggcgga actcgcggtt gaaaaacatc gccactgcca
     8041 gcccgagcac ggtgctgccc gccaccacca gcgcggtata ccagaccgtc atccacagcg
     8101 agtgccagaa gccgggatcg gagaggatac gcacatagtt actcacccca acaaaggttg
     8161 actcgatgtt gggattgagc ggcagacgta aaaaactgat ttcgatattc gacaccattg
     8221 gccaggccac cagaccaccc agcagtaata agctgggcgc gagaagcagc agcgcaaagg
     8281 gcatatcgga acgacctgaa aaaagcctgt tcattgcgga ttcctgtcct gggtttagtg
     8341 ctgttcgatc agttcatcca gctttttctg gctcgcttgc agcgtgccag ggagatcggc
     8401 tttacccacg gtgacgttat gcaccatgga gctcaccacg ccagaacccg tcacatcacc
     8461 catgcgggta aagtttttat cccctactgc gccaaagacc tgaatatttg gcagttcacc
     8521 gattagctga ttcggtagtt cacccagcgc cttaataacg tcgttgtctt tccaggtggc
     8581 ggtagtgaca accgctttat tcaccggcag cgcagcacct ggcgacatca tcacccagtc
     8641 ggcaatgttg tctgcctgct ccataaaggt gacaaatttt tctgctgctt ccgtctcttc
     8701 ggccttttgc ccggcggtaa tggtcagcga ggtcaacatg ccgtagaccg cagagttttt
     8761 ctcggttggc accacgaaac cgacgttttt cgggtcgcct tctttaatta cagccggaag
     8821 gatataggtg gagtaaatcg ccatcggcgc ggtgccgttc ataaaggcgt ctttcacttc
     8881 catgatgtcg ttagaacccg gcatgtcgtt agaacccggc atagtgttgg cagcaaggtc
     8941 gcggtaatag gtcagtgcct gcatcatctc tggtgtatca agggtgattt tgccttcggc
     9001 gttaaagacg ttagcctggt tggataacgc aaactgggag aaggattgtt ccgtcaacac
     9061 gctttctgct gtaggcagcg caatgccgta ttttttattc gccgggtcat tcagtttctg
     9121 tgcaacgtcc agtagctgtt gccagttttt cggctcctcc agccccgctt ttgccagcac
     9181 atctttgcga taccagatgc cgccaatcca ggcgctgacg ggaacaccgg tccaggcact
     9241 cccatcttcg gtgcgcacaa tacgcagtac gccatcgtaa aacgcgcctt caccaacgtt
     9301 gctgatgacc gtggcaaccg tcttgcgatc gataagctgc tctttatcca taactttggc
     9361 gtagtcatgg ctggtttcga tcacttccgg cagcgagccg ctacgtgaaa gggtgatgac
     9421 tttggtgtta taagcgtcct cttccacggg cacttgttta acggtgatac ctgggttttc
     9481 tttttcaaaa cgggcaatca atttgctgat aactgcctga cgctcctgct ccaccgaaga
     9541 atgcataaat tcgatggata catgggtttt attttcttct ttacagcctg aaatcagggc
     9601 gcaggaaacc agtgctgata acagcacgat ttttgattta atcatgagat gttccttttt
     9661 atttaatcca cataacctgc caggggcgca gcgttatttc attacccaat agatcaactt
     9721 cactaatcag atcccgacca tgcagattag taatatttac atgctgaata tttttactga
     9781 cattaaacaa tccagtcaga caattaccat cagcgttact tctttgaata cgcattacgg
     9841 atgaattaat cgtatcgatg gtaaaattat tatccggatg aaactcgtta tggctgcgac
     9901 gaagtgtaat taaacgcgac aactcatgat ataccgcatg ccttaatgta gcttcatcgt
     9961 tcagttctcg ggttatctct ttactgtgat attttttacg gttaatcgca cggttatatc
    10021 cgagtttttc gacacctgcg taatcattac gcgagccaag aatactttga atatatatcg
    10081 ctggcacacc ggggaaactt aacaaaatcg catgggcgag aataaacctg gcgcaacgtt
    10141 cttcatcgct actctcacgg cggcttaacg catccatatg tgagaggcga ggaagttaaa
    10201 ccaggtggtg ttgctggaag gtagtgtcag gttttgcgcc cacgcacaaa gcgcctcaac
    10261 gttctgtttt tgcaccgcat gcagcaccag tggcggcagc gagaactggt acaccatatg
    10321 tgcttcgtca tcgcctgcgc caaagtaagc aatgttgtct ttatgcggaa cattggtctc
    10381 ggtaatgatc actgtacctg gcgcaacgtt atcaataatc gaccgtaaca gtttgataat
    10441 cagatgtgtt ttttccagat ggatgcagct tgttcccggc tctttccaca taaagccaac
    10501 ggcatccagg cggacatact cagcaccttt cgcaaggtaa cagagtaaaa catccaccat
    10561 cgccagcaac acttcagggc tacggtaatt caggtcaatt tgatcgtcac taaaggtggt
    10621 ccataaatgg cgcgttgaat gatcgcgcat ctggaatggc gttaataacg gtaacgcacg
    10681 cgggcgagtg acggcgctga gatcggtttg cggatcaacc gcaataaaaa aatcttcaaa
    10741 acctggctgc tgttgtaaat agtttttaaa ccattcactt tttgccgaca tatggttgca
    10801 gacaaaatca aacattaaat gactgcattc accgagttgc tgaatatcct gccactcccc
    10861 cgcttcactg gcgacctgat gataatcaat taccgaaaac ccatcatcag atgaacatgg
    10921 ataaaaaggt aataaatgaa catgtgaaaa aatgctttgc agccattgat ggtaaaactg
    10981 attaaatgtg ggtaatggtt ttaaatcatt gctgtgaaat tgatcggcat aggtaattaa
    11041 cacgacatca ctttcatccc agtgtttttt acgtcgctgt gtaattaatc gtttcgcact
    11101 ctcaagacgc gttacaagtt tctgtaaatg agttgcggta aatgttccac cgtagatttc
    11161 gtccaggtaa tccgtaattt tctgtttcat gttgcctttt tgtggtagcg gtcccatgaa
    11221 acgagagact actccgcaga aaataaactg tcaattgagc acaagggttt tagtcacgca
    11281 ggaaatcatt ttttaccagg gaaaaagcgt atttgtgagc tgacgcaaat aatctatggg
    11341 gatgtgaata aggtgtgaca gaaaaaaaac ggcgcataag cgccgctcat ggtgaattct
    11401 tttaaccttt gaccttcggc attgcgatgt ctttcaggcc accggcgttc tcaacgtggg
    11461 tatatcccat ctcgctaagg atctcttttg cttgccctga ctggcgtccg gcattgcaat
    11521 acactttcac ggtgtcgttt ttatccggaa cggcggtggc aatgcgctct ttcacttcct
    11581 tcaggggaat attgatggcc ccctgaacgt gctcttgctg atactgctct ggaacacgaa
    11641 catcgatcca gtgttcagcg gcgaaaacgg gcagtgaaaa caccagtgcc agagcaagta
    11701 agcctttttt aaacatgtaa gcgtccttct atggatgaaa attgccatcc attatacgcc
    11761 tgagatttaa caatttttgc gaaactcccc tcacctttta taacgctgtg ccagtttatt
    11821 agcagcccga cttaacagcg gttccagcgc cacagccagc aacattttca gcggtcggcg
    11881 agcaactgat tttatcgccc agcccgctac acccgccggg ccatagcgca gtgcggtaag
    11941 aagtaccagc ttgcctgcta atttgaaacc aggctttacc ttttgcccgg cctgttgcca
    12001 gcgagtattc ataactttcc tcacagttga cggaaacggc tacgtaacgt gaaagtatcg
    12061 gaagtgacat aacgttccat ctcgcgtaaa cgcgtttcac ttgccgccag ttcacggtcg
    12121 acttcatcca gcaattcgct gctggaaggt agctgctcac caaaggccat gttgtccggc
    12181 attggatcaa gcgcaaatga caaaatgatg taagcaacca gggtaaacag cgccagaccg
    12241 aagaaaatcg acagcaccac caggatacgc accagtttta ccggtacatc aaaatagttg
    12301 gcaatcccgg cgcagacgcc gcggaccatg ccctgctgtg gaatacgcca taatttttta
    12361 ttgagattaa tgcccgccat tagcgatccc tccagttcgg atgttctgca tcaagaatag
    12421 attccagcgc ctgaatacgt tcgcgcatcc gttttgcttc atcagccagt tgcgctaatc
    12481 gctgctgctc actttgcgac aattcatcgc gaccagaacg attgctgtaa tgcagccata
    12541 accagatcgg taaaacaaac agcacaaaaa tggttaacgg aatagccaga aatagcgcgc
    12601 tcataagtac tccttagacg atgagcggcg gcgcgttcgg acgccgcctg gatgttatta
    12661 ttgattgtct tgcttcattt tggcttttaa ttgtgccagt tgttcgctga ttgcatcatc
    12721 ggctttcagt tcggcaaact gatcgtccag cgatttttgt ttaccgaagc tgtggctttc
    12781 tgcttccgct tccatctggt caattcgacg ttcgaaagat tcgaaacgag ccattgcttc
    12841 atccagtttg ccactgtcca gctgacgacg cacatcgcgc gacgagtttg ccgcctgatg
    12901 acgtaacatc aatgcctgct ggcgagcgcg tgtttcgctc aatttgtttt ccagctcgcc
    12961 aatctctttc ttcatgcgtg ccagcgtatc gtccaccagc gtcacttcat gttccaggga
    13021 cttaatcaga tcggtcagtt tctgtttttc aattaacgct gcacgtgcca gatcctctct
    13081 ctctttcagc agcgccagtt cggctttttc ctgccattca acttcacgcg ccgacgcttg
    13141 ttcaatacgg cgagtcagct gtttcttttc tgccaacgca cgcgccgaag tagaacgtac
    13201 ttcaaccagt gtatcttcca tctcctggat catcagacgc accagtttct gtggatcttc
    13261 cgctttctct aacagagcgt tgatgttggc attcacgatg tcggcaaagc gagaaaaaat
    13321 acccataatg ttgtcctctt gatttctgcg ttcaggattg tcctgctgaa ctgttaatac
    13381 aatttgcgtg ccaatttttt atctttttga tttataaaga tctgattgaa gaatcaacag
    13441 caacatgcca ggatgaatta gcgaattaca ctaacaagtg gcgaatttca tcatggccga
    13501 atacaaagat aatttacttg gtgaggcgaa cagctttctc gaagtgctgg aacaggtttc
    13561 gcatctcgca ccgctggaca aaccggtgct catcatcggc gaacgcggca ccggtaaaga
    13621 gctgattgcc agccgcctgc attatctctc ctcccgttgg caagggccgt ttatttccct
    13681 taactgcgcg gcgttaaatg aaaatctgct ggattccgaa ctgtttggtc acgaagcggg
    13741 ggcgtttacc ggtgcgcaaa aacgtcatcc agggagattt gaacgtgccg acggcggtac
    13801 gctatttctt gatgaactcg ctacggcacc gatgatggtg caggagaaat tattgcgcgt
    13861 gattgagtac ggtgaactgg agcgcgttgg cggcagccag ccattgcagg tgaatgtgcg
    13921 gttggtatgc gcgacgaatg ccgatctccc ggcgatggtc aatgaaggca cttttcgcgc
    13981 tgacctgctc gaccgactgg cttttgatgt tgtacaactg ccaccactgc gcgagcgcga
    14041 aagcgacata atgttgatgg cagaacactt tgccatccag atgtgtcggg aaatcaagct
    14101 gcctctgttc ccggggttta cggagcgcgc cagagaaaca ttgctgaatt atcgttggcc
    14161 gggaaatatt cgtgaattga aaaacgcggt ggaacgttca gtgtatcgcc acggcaccag
    14221 cgattatccg cttgatgaca tcattattga tccctttaaa cggcgtccgc ctgaagacgc
    14281 tatcgccgtt tcagaaacca cctcgcttcc gacgctgccg ctggatttac gtgagtttca
    14341 aatgcagcag gaaaaagagt tgctgcaact cagtttgcaa aaggggaaat ataaccagaa
    14401 gcgcgcagct gaattgctgg ggttaaccta ccatcagttc cgcgcgttgt tgaaaaaaca
    14461 ccagatttag cgaagttggg caatgcgatt cgccggatgc ggcgtgaacg ctttatccgg
    14521 catacataat caacgtcgtt tgcaggcctg ataagcgcag cgcatcagac attattgcgt
    14581 tgggctatta atcgctcagc gcatcctgca aaattttcat tgccgcatcg aattgcgcat
    14641 ccgggatggt cagcggatac aggaagcgaa tcacgttgcc gtatgcgcca caggtcagca
    14701 ggagcagccc ctgcgccagc gcgcgttgct ggattttctg tgcaatcgcc gctgacggct
    14761 cgcccgtttg cggatcgtta aactctgccg caatcatcga ccccaggccg cgtaccgcag
    14821 caatggccgg aacgctttct ttggcatcaa tcaacgtgtt tttgagacgc tggcccagtt
    14881 gattcgcgcg ttcgcagagt gattctttgt tgataatatt gagcaccgcg tgcgcggcag
    14941 ccaccgccag cgggttcccg gcgtaggtgc cacccaaccc gcccggcgcg ggcgcgtcca
    15001 taatattcgc gttaccgacc acgcccgaaa gcggcatccc gcccgcgagg cttttcgcca
    15061 tcgtcattaa gtccggctta tcggcgtaat gatccatggc aaacagttta ccggtacgcg
    15121 caaagccgct ttgcacttca tcggcaatca tcacaatacc gtgctcgtcg cacaggcggc
    15181 gaatggcggc aaccagctct tttggcgcaa cgttgaaacc gccctcgccc tgcaccggtt
    15241 cgaaaataat ggccgcgacc tgcttcgctt cgatgtctga tttaaacaag cgttcgatgg
    15301 cgtcgaggga gtcctatgtt gaaatgccgt gtaaatctga cggataaggt acgtgataca
    15361 ccgaaccagg gaacgggccg aagccgattt tgtacggcgc aacttttccg gtcagcgcca
    15421 tggtcatata tgtacgaccg tggaagccac cgctaaacgc aatcacgcca gggcgtccgg
    15481 tatgggcgcg agcaattttc accgcgtttt ccaccgcttc cgcaccggta gtgaagaacg
    15541 cggttttggc ctgcccgctc accggggcaa gggcgttgat tttctccgcc agggtgacgt
    15601 agctttcata cggcacaatc tgatacgcgg tgtgggtaaa ctgttgcagt tgctgctcca
    15661 ccgccgcgac cagatcaggg tggcgatgtc cggtattcag caccgcaatg cctgcggcga
    15721 aatcgatgta ctcgttgccc tcaacatcct tcagagtagc gttttcagcc gactgggcga
    15781 agaagttaca catcacgcca accccgcgtg gggtagcaga aagacgacgc tgatggaatt
    15841 cattgttgct catgtttcac accttttgac tcttcactgt taatcggaaa ttaaacgccc
    15901 agacgatcgc gcaggctgta ccaggctgcg cccatcgcgg taaacggtac tcgcagcgtg
    15961 cgcccgccgg ggaacgggta atacggcaga ttggcgaagg catcgaaacg ttcggcgtcg
    16021 ccgcgcagca gttcggcaat caaacgtccg gcgagatgag tacaggtgac gccgtggccg
    16081 ctgtaaccct gcatgtaata gatgttggta tcaaggcgac caaactgcgg catgcgcgac
    16141 agggtcagca ggaagttgcc cgtccagcgg tagtcgattt tgacgccctt cagttgcggg
    16201 aaggttttca gcagtttcgg cacaacaaga cgctcaacgt catccgggtc gcgcgcgccg
    16261 tagaccacgc cgccgccgta cagcaaacgg ttgtcggcgg taagacggta gtaatcaagc
    16321 agataattac agtcttccac acagtagttt ttcgggatca gcgaacgggc taaatcttcc
    16381 gacagccgtt cagtggtgat cacctgcgtg ccgcacggca tgctgcgttt cgccagttcc
    16441 ggctctactt tatcgcccag atacgcattc ccggcgacaa tcacatactt cgccgtcacc
    16501 tgacctttgg cagtgcgcac caccgctggc gtggtgtgct ggatctgcgt cacggcagaa
    16561 agctcataca cgcgcccgcc gttcaggcgg atggcatcgg cttcaccaat ggcgaggttt
    16621 agcgggtgaa tatgcccacc gctgtgatcc aacagtgcgc cagtgtagcg atcgctggcg
    16681 acctcgcggc gaatggcgtt agcgtccagc agctccagct gtttattgcc gtagcgttcc
    16741 cagttctctt tctgctcttc gagtgtggcg agctgtttat cattcatcgc cacaaacagg
    16801 ccgccggggc ggtagtcgca gtcaatctga tagcgtttga tacgttcgcg gatgatctcg
    16861 ccaccctcga acatcatgct gccgagcata cgggcggtgt ccatgccgta gcttttttcg
    16921 atcacgtcga tgtcgcggct gtaggagttc acaagctgcc cgccattgcg cccgcttgcg
    16981 ccaaagccga tgcgtgaggc ttcgagaacc actacgtcaa agcccgcttc cgccagatgc
    17041 agcgcggagg agagcccggt atagccgccg ccaaccacgc aaacgtcgca ggtgattgac
    17101 tcattcagtg tgtcgaatgg tgcatattta ttcgcactgg cggcgtagta actgctggta
    17161 tgttcggtca tttcaggcct ccaggcttat ccagatggtt ttcagttcag tgaatttttc
    17221 aagggcatgc agggatttgt cgcgaccgtt gccgctctgc ttatagccgc caaacggcac
    17281 ggtcatatcg ccgtcgttgt agttattgac gaagacggaa ccggctttca ggcggcggct
    17341 catacggtgc gcgcgggaga ggtcgcgcgt ccataccgcc gcgccaaggc cgtactggct
    17401 gtcgttggca agctgtagcg cctggtcttc tgatgtgaaa cgcgtgacca ccagcaccgg
    17461 accgaaaatc tcttcgcggc ttaaggacgc attcgggtca acatccacaa agatggtcgg
    17521 gccgatggcg gcagccagct cagcgttacg accatccagc agcagttgcc ctttgctttc
    17581 gccttcacga ataaagctat ggaccgagtc ggcgtgggcg caatcgatta aggtgcccat
    17641 ggtggttgcg gtatcaagtg gatggcccgg ctgccagttt tgcgcctgct gttttaacag
    17701 ggctaagaat tcatcggcga tgctctcttc cagtaacaaa cgcgttccgg cgatgcacac
    17761 ctggccctgg ttgtagaaga tgcctgcggc ggtggcgctt gccgcttttt gcagatccgg
    17821 acaatcggcg aaaacgatgt tggcgctttt gccgcccgct tccagccaga cgcgtttcat
    17881 gttgctatca cccgcgtctt tcagcagttg tttacccgta cgcgtggagc cggtaaaggc
    17941 gatggcatcg atatcgttat gacgcgaaag cgcctgcccg gcttcatgac cataaccgct
    18001 cactacgttc agcacgccat ccggcagccc cgcttcttct tttgccagcc cggcgagacg
    18061 aatcgcactg agcggtgatt tttcagacgg ttttagaatc acgctgtttc ccgccgccag
    18121 cgccgggccg agtttccagc aagtcagcag cagcgggaag ttccacggca cgatggcggc
    18181 aatcacgccg accggttcac gcacgatcat cgccagctca tggctactgg tggtcgccac
    18241 ttcgccatac actttgtcga tcgcttcggc gtaccagcga atggcgcgcg ccgcgccggg
    18301 aatatcatca cgcagactgt gacgaatcgg tttgccggtg tcgagagttt ccagcagtgc
    18361 cagctcttcg gcgtgggctt ccattaaatc ggcgagttta ttcagtaccg ctttacgttt
    18421 agccggagaa gagagtgacc agtcgccgcg ttcaaatacg ccgcgtgctg cgctcaccgc
    18481 atggtcgata tcgacgctct tgccgcgggc aattttcgcc agcggtgcct gggtgaccgg
    18541 atcaacggtt tcaaaggttt cattttccgc cgcagcagta tattcaccgt taataaataa
    18601 gcggttttca atggcgagac ttaacgcttt atcctgccag taagccagat gatgaaaatt
    18661 cattatgact cctgtttcac gtctatcaga tatatgcagt ttgtgaacgt cagggcgcag
    18721 agatccccgg gccgcatgcc cgtttatttc tggcgacatt ctttatggat tgccgggcgc
    18781 attgcgctta cccggcttac agaacaaaaa gattaaaacg tggtgggcgt atgggcgctg
    18841 ataattcggc aaatacctgc cgacgtatta ctgaaactgt gcgggatgcc ggtattaatg
    18901 gcataacttt gccccgcgac gaggtggtaa tcctgaccat taatcgtcag aacaatttca
    18961 ccttccagta ctgtgcctat ttcctcaccc tgatgcttaa ttctttcccc agtggttgtg
    19021 cccggctggt acgtttcaaa gatcatcgcc agcgtgcgat tcgggttacc gttatgaacc
    19081 agcttcattg acacaccctg actgcccatc tcaattaagt cgtcctgatt aatgacgacc
    19141 tgcggctcat caggtttttc cggctcggaa aagaattccg agagtgacag accatacacc
    19201 ttcagcagct tttgcagcgt actgatggca gggctgactt tatcttgttc tatcgtactg
    19261 atagcactgt gagtcagccc ggagagttcg gcggcacgac gttgtgaaag cccctgctgc
    19321 tggcggattt ccgacaagcg ttttcctggc gccagtccct catcactcat atttgcattt
    19381 ccttaaactg tagtggtcag agtcgctgtt tttcagcgat atggtgctga caagcggtga
    19441 taaagccctc gaacaatata cgcgaaagcg cgtactcgct actgttccat tccgggtgcc
    19501 actgtacgcc cagcgcaaaa ggatgattga tgacgctaac cgcctcaact aagccatccg
    19561 gcgagcgagc ttcaacacgc aaccgtgggc taacgacctt cgccccttgt ccatgtagag
    19621 agtttaccca aaagttgcta cattcaggta acaacgcaga cagtaatccc ccctcttcaa
    19681 cctgaacttc atgcgacggt gcatattgct gttccaccgg cagttcggga tcttcccgat
    19741 gttccagcaa ttcaggctgt tcgcacagct tgcgatgcaa cgaaccaccg gttgccacca
    19801 ccagttcttg taaaccccgg cagatggcga aaatggggat gcgcctttcg agtgcggcat
    19861 taattatcgc catgctcaga agatcacgcc cgggatcggc gtcaggctca tcgccgtttt
    19921 caccatatag gtgcggctgc acattgctgg gactaccagg aagataaatg ccatcgagtt
    19981 tcggcaaaag ttgttcaagt aacgacggtt ccgccagcgc atgtggtagc gcaattggca
    20041 agccgcctgc gtggatgatg gcattcaggt acttttcttg cagagtctgg gtcgcatgac
    20101 ccttaagcct gttcctgcac attacgacac cgataaccgg attgttcatt atattttcca
    20161 tgctcaatct cacaaagtgg actaaattat cgccattact gcgctttaaa tgacctttat
    20221 gttcaatatt ttttcaatct agcagtgggt tttcattttt gcaaactcaa tttaacattt
    20281 gacaaacatt tagtttgcat acagattcga atggtggtca ttatatttta cgctttgata
    20341 acgagcggaa aacaaaccaa aggcgaagaa tcatggaaac caatatcgtt gaagtagaga
    20401 actttgttca gcagtcagaa gagaggcggg gtagcgcctt tacgcaggaa gtgaaacgct
    20461 acctggagcg ctacccgaat acgcaatatg ttgatgtttt acttactgat ttgaatggtt
    20521 gcttccgtgg aaaacgtatc cctgtttcaa gcctgaagaa gctcgagaaa gggtgttatt
    20581 tcccggcctc ggtatttgca atggatattc tgggcaacgt agtagaagaa gcaggtctgg
    20641 gtcaggaaat gggcgagccg gatcgtacct gtgttcctgt tctcggttcc ttaactcctt
    20701 ctgccgccga tccagagttt atcggtcaga tgctcctgac catggtcgat gaagatggcg
    20761 ctccctttga cgttgagccg cggaacgttc ttaaccgcct gtggcagcag ctgcgccagc
    20821 gcgacgctga agggaatctg caaccgccct gcgcgcctgg caccgatgac cgcaatacac
    20881 aaagccaggt ttactccgtt gataacctca accacttcgc cgacgtgctc aatgatattg
    20941 atgaactggc gcagttgcag ctgattccgg cagatggcgc ggtcgctgag gcctcgccgg
    21001 gtcagtttga aatcaacctt taccatactg ataacgtgct ggaagcctgc gatgatgcgc
    21061 tggcactaaa acgcctggtg cgtctgatgg caggaaagca taagatgcac gccactttta
    21121 tggcgaagcc gtatgaagag cacgcgggca gcgggatgca tatccatatc agtatgcaaa
    21181 ataatcgtgg cgagaatgtg ctttctgaca cagaaggcga agattcgccg ctgctgaaaa
    21241 agatgctcgc cgggatgatt gacctgatgc cgtcgtcgat ggcgttgctg gcaccaaacg
    21301 tgaactcgta tcgccgcttc cagccgggaa tgtatgtgcc gacgcaggcg tcgtggggcc
    21361 ataacaaccg caccgtcgcc ctgcgtattc cgtgcggcga ccgtcataat caccgcgtgg
    21421 aatatcgcgt ggcgggtgcc gatgccaacc catatctggt gatggcagcg atttttgccg
    21481 gtattttgca tggccttgat aacgagctgc cgttgcagga agaagtcgaa ggcaacgggc
    21541 tggaacagga aggcttaccc ttcccgattc gccagagcga tgccctgggt gagtttatcg
    21601 agaatgatca cctgcgccgc tatttaggca aacggttttg ccatgtgtat cacgcctgta
    21661 aaaatgatga gctgttgcag tttgagcgcc tcatcacaga aacagaaatt gagtggatgt
    21721 tgaaaaacgc gtaattcccc ttgcggggtt gtttcatcgg cctgagcgga gccaggcctg
    21781 acatttgctg gtaatccaga catttcccgg atgtcgcgtt atggagcgca ggcggcaacg
    21841 ggcttccgtt aacgattaag actgtgacga ggaaatgaga tgatgccgat gattaaatcc
    21901 ccgcatggcg agggaggctg cgtatgcgcc cctcctgcta ctgactggac tccgccccct
    21961 ttgcttccct tgctaaacag gtttgatttc aggtcgacac gaccgcaaac cttattacgc
    22021 aggggaggca gcaattatgg ctattaattc accactgaat attgctgcgc aacccggcaa
    22081 aacccgtctg cgaaaatcac tgaaattgtg gcaggtggta atgatgggtc tggcctatct
    22141 cacgccgatg accgtatttg atacttttgg tattgtctcc ggcattagcg acggtcacgt
    22201 tccggcgtcc tatttgctgg cgctggcggg cgtgctgttt accgctatca gctacggcaa
    22261 actggttcgc cagtttccgg aggccggttc ggcctatacc tacgcgcaaa agtcgattaa
    22321 cccgcacgtc ggatttatgg tcggctggtc atcactgctg gattatctct ttttgccaat
    22381 gatcaacgtt ttgttggcga aaatctatct ctccgccctc ttcccggaag tgccgccgtg
    22441 ggtgtgggtg gtaaccttcg tcgccatttt aaccgccgcg aatctgaaga gcgtcaacct
    22501 ggtcgctaac ttcaataccc tgtttgtact ggtgcaaatc tccatcatgg tggtgtttat
    22561 cttcctggtg gttcagggac tgcataaagg agaaggcgtt ggcaccgtct ggtcacttca
    22621 gccgtttatc agcgagaacg cgcacctgat cccgattatt accggtgcga cgattgtctg
    22681 tttctcgttc ctcggtttcg atgcggtgac cacgctttcg gaagagacgc cggacgccgc
    22741 acgcgtgatc ccgaaagcca tcttcctgac ggcggtatac ggcggcgtta tctttatcgc
    22801 ggcgtcgttc tttatgcagc tgttctttcc cgatatcagc cgctttaaag acccggacgc
    22861 cgcactgcct gaaattgcgc tgtacgtcgg cggcaagctc tttcagtcga ttttcctctg
    22921 caccacgttt gtgaacacat tagcgtctgg cctggcttca catgccagcg tgtcgcgtct
    22981 gctgtatgtg atggggcgtg acaatgtgtt tccggagcgc gtgtttggct atgtgcaccc
    23041 aaaatggcgg actccggcac tcaacgtcat tatggtcggg attgtcgcgc tgtcggcgct
    23101 gttctttgat ttagtcaccg cgacagcact gattaacttc ggtgctctgg tggcgtttac
    23161 cttcgtgaat ctgtcggtgt ttaatcattt ctggcggcgt aaaggaatga ataaaagctg
    23221 gaaggatcac ttccactatt tgctgatgcc gctggttggc gcgctgacgg tgggtgtgct
    23281 gtgggttaac ctagagtcaa cgtcactgac actcggtctg gtatgggctt cgttaggcgg
    23341 cgcatatttg tggtatctga tccgccgcta tcgcaaagtg ccgctgtacg aaggtgacag
    23401 aacgccggtg agcgaaacgt aatagccgga tgcgcggcgc attatcctgc ctgagggtaa
    23461 cgcgccgcgc cattaactat aaaaagatgt atgacccgga tcacgagtgc tatcatccgc
    23521 ccttatgctt agcgcattaa ccccaggggc aaataatgaa ccacgatatc ccgctaaaat
    23581 attttgatat tgccgatgag tacgcgaccg aatgcgcaga gcctgtcgca gaggctgaac
    23641 gcacaccgct ggcccactac ttccagttac tgctcacccg tttgatgaac aatgaagaga
    23701 tcagcgaaga agcccaacat gaaatggctg ctgaagcggg gattaatccc gtacgcattg
    23761 atgagatcgc agagtttttg aatcaatggg gtaatgagta attatttaat tgcagacgat
    23821 cggtcagtat cgttgtccgg ataaagcgtt cacgccgcat ccggctaaca atgacacgca
    23881 ctgcagcgtg ctttaatggg tttctcacga tcgtaccgct ctgctcctcg ctaagttgtt
    23941 ctgtgagtta tagttataga gaaatcacgg gtacgaatgt tgaacagatg gcgacgccac
    24001 ctgttcaacg accaaccaca cggccctcat tacgacccct gaacacaaac ggtaatggca
    24061 aatgtcatta aatattagta agatagcctc tttacataaa atcccttaat attatgcgcc
    24121 aggtattatc gtctcttttg gtgattgctg gacttgtgag tggtcaggca atcgccgcgc
    24181 ctgaatcccc cccgcatgct gatatccgcg acagcggttt tgtctattgc gtcagcgggc
    24241 aagtcaacac ctttaaccca tccaaagcga gcagtgggtt aattgtcgat acccttgccg
    24301 cccagtttta tgatcgactg ctggatgtcg atccctatac ctatcgcctg atgccggaac
    24361 ttgccgaaag ctgggaagta ctcgacaacg gcgcgaccta tcgcttccac ctgcgtcgcg
    24421 atgttccgtt tcaaaaaacc gactggttta ctcccactcg taaaatgaat gccgacgatg
    24481 tggtgtttac cttccagcga atttttgacc gcaacaaccc gtggcataac gtcaacggca
    24541 gcaacttccc ctacttcgac agcctgcaat ttgccgataa cgtgaaaagc gtccgcaaac
    24601 tggataatca taccgttgag ttccgactcg cccagccgga tgcttctttt ttgtggcacc
    24661 tcgcaaccca ttatgcttcg gtcatgtcgg cagaatatgc ccggaagtta gagaaagaag
    24721 atcgccagga gcaactcgac cgtcaaccgg tcggcaccgg tccgtatcag ttgtcggaat
    24781 accgcgccgg gcaatttatt cgcctacaac gtcatgatga cttctggcgc ggtaaaccgt
    24841 taatgccgca ggtagtggtg gatttaggct ccggcggcac cggacgtctg tcgaaactcc
    24901 tgaccgggga atgcgacgtt ctggcctggc ctgctgccag ccagctatcc attttgcgtg
    24961 acgacccgcg cttgcgttta acgctgcgtc ctgggatgaa cgtcgcctat ctggcattta
    25021 acaccgccaa accgccgcta aataatcccg ctgtccgcca tgcactggca ctggcgatta
    25081 ataaccagcg cctgatgcaa tccatctatt atggtacggc tgaaacggcg gcctctattt
    25141 taccgcgcgc ctcgtgggcc tatgacaacg aggctaaaat tactgaatac aatccggcga
    25201 aatcgcgcga acagttgaag gcgttggggc tggaaaactt aacgctgaaa ctgtgggtgc
    25261 ccacacgttc gcaggcgtgg aaccccagtc cactgaaaac tgccgaactg attcaggcgg
    25321 atatggcgca ggttggcgta aaagtggtga ttgtgccggt agaaggtcgc tttcaggagg
    25381 cgcggttgat ggatatgagc catgatctga cgttatccgg ttgggcgacg gacagtaacg
    25441 acccggacag tttcttccgt cctttactga gctgcgcggc aattcattca cagaccaacc
    25501 tcgcccactg gtgcgatccg aaattcgaca gcgtgttgcg taaggcgctc tcctcgcagc
    25561 agctggcggc gcgtattgaa gcctatgacg aagcgcagag tattctggcg caggaattac
    25621 ctattctgcc gctggcgtcg tcattgcgtt tgcaggccta tcggtacgat atcaaaggtc
    25681 tggtacttag cccgtttggt aacgcctcct ttgctggggt gtatcgcgag aaacaggatg
    25741 aggtgaaaaa accatgatta tcttcacctt acgccgcatt ttgttattga ttgtcacctt
    25801 gttcctgctg accttcgttg gcttcagctt gagctatttc acccctcatg cgccgttaca
    25861 aggcgcgtca ctgtggaatg cctgggtgtt ctggtttaac ggcctgatcc actgggattt
    25921 tggtgtttcc agcattaacg gtcaaccgat tgccgaacag ttaaaagagg tcttcccggc
    25981 gacgatggaa ctgtgcatcc tcaccttcgg ttttgcgctg attgttggca tccccgtggg
    26041 gatgattgcc gggattacgc gccataagtg gcaggataac ttgatcaacg ccatcgcctt
    26101 actgggcttt tcaatcccgg tgttctggct ggcgcttctg ttgacgctgt tttgttcact
    26161 cacgctaggt tggttgcccg tttcagggcg tttcgatctg ctctacgaag tgaaaccgat
    26221 taccggtttt gcgttgattg atgcctggct ttcggactca ccatggcggg atgaaatgat
    26281 catgagcgca atccgccata tgatattgcc cgtgatcacc ctgtcggttg cgccaacaac
    26341 tgaagtgatc cgcctgatgc gtatcagcac tatcgaagtg tatgaccaga actatgtaaa
    26401 agcggcggcg acccgcggtt tgtcgcgctt tactattttg cgtcgccacg ttctgcataa
    26461 cgcgttaccg ccggttattc ctcgcctggg cttacagttt tccaccatgc tgacgctggc
    26521 gatgatcacc gaaatggttt ttagctggcc gggtttaggg cgctggttaa ttaacgccat
    26581 ccgccagcag gattacgcag ccatttccgc cggagtgatg gtgtgtggct cactggtcat
    26641 tattgttaac gtgatttctg atattttggg tgccatggct aaccctctga aacataagga
    26701 atggtatgcc ttacgatagc gtatacagcg aaaagcgccc gccgggcacg ctgcgtaccg
    26761 cctggcgcaa attttatagt gacgcctctg cgatggtcgg cctgtacggc tgcgcggggc
    26821 tggctgtact gtgtattttt ggcggctggt ttgcgcctta cggcatcgac cagcaatttc
    26881 tcggttatca attattgccg ccatcatggt cgcgctatgg cgaagtttct ttcttcctgg
    26941 ggactgacga cctcggacgc gatgttttaa gccgtttact gagcggagct gcgccaaccg
    27001 tgggtggcgc atttgtggtg acgcttgccg cgacgatctg tggcctggta ctgggcacct
    27061 tcgccggagc gacacacggc ctccgctcag cggtgcttaa ccatattctg gataccttac
    27121 tggcgatccc ttcgctgctg ttggcaatta tcgttgtagc gtttgccgga ccgagtttgt
    27181 cccacgctat gtttgctgtc tggctggcat tgctgccgcg tatggtacgt tcgatttaca
    27241 gcatggtgca tgacgaactg gaaaaagagt acgttatcgc cgcccgtctg gatggcgcat
    27301 caacgctgaa tattctctgg tttgctgtga tgccaaacat caccgccggg ctggtcaccg
    27361 agatcacccg cgcactgtcg atggcaattc tcgatatcgc cgcgctgggc tttctcgatc
    27421 tcggcgcaca actcccctcg cctgaatggg gagcgatgct cggtgatgcg ctggaactga
    27481 tttatgtcgc cccgtggact gtcatgctgc caggtgcggc aattatgatt agcgtgttgt
    27541 tggttaacct ccttggcgat ggcgttcgtc gtgcgattat tgcgggggtg gaataatgcc
    27601 attactcgat attcgtaacc tgaccattga atttaaaacc ggtgatgagt gggttaaagc
    27661 cgtcgaccgg gtaagtatga ccttaaccga aggtgaaatc cgcggtcttg ttggtgaatc
    27721 cggttccggc aagagtttga ttgcgaaagc gatttgtggg gtgaataaag ataactggcg
    27781 tgttactgct gaccgtatgc gttttgatga tatcgatttg ctgcgtctct ccgcacgcga
    27841 acggcgcaaa ctggttggcc ataacgtgtc gatgattttc caggaaccgc agtcgtgtct
    27901 tgacccttca gaacgtgtgg gtcgccagtt gatgcaaaac atcccagcct ggacctataa
    27961 aggccgttgg tggcagcgtt ttggctggcg caaacgccgt gccattgaac tgctgcaccg
    28021 cgtggggatt aaagatcaca aagatgcgat gcgcagtttt ccctatgagt tgaccgaagg
    28081 tgaatgtcag aaagtgatga tagccattgc actggcgaat caaccgcgtc tgctgattgc
    28141 tgacgaaccg accaactcaa tggagccaac aacccaggcg caaatctttc gcctgctgac
    28201 gcgtctcaac caaaacagca ataccactat tttgcttatc agccatgact tacaaatgct
    28261 tagccaatgg gcggataaaa ttaacgtgct ttactgcggt caaacagtgg aaaccgcgcc
    28321 gagtaaggag ttggtgacga tgccacatca tccttatacc caggcgctga tccgcgcgat
    28381 accagacttc ggcagcgcga tgccgcataa aagtcgcctc aatacgctgc ccggcgctat
    28441 cccactgctg gaacagttac cgattggttg tcgtctgggg ccacgttgcc cgtatgcaca
    28501 acgagaatgc attgtgacgc cacgtttgac gggggcgaaa aatcatctct atgcctgtca
    28561 tttcccgctg aacatggaga aagagtgaga tgatcgaaac gctgcttgaa gtgcgtaatt
    28621 taagtaaaac gttccgctac cggaccggct ggtttcgtcg tcagaccgta gaagcggtaa
    28681 aacccttgag ctttacgcta cgtgaaggcc agacactggc gattattggc gagaatggtt
    28741 cgggtaaatc cacgctggca aaaatgctgg cgggaatgat agagcctacc agcggcgagt
    28801 tattgattga cgatcatcca ctgcattttg gcgattattc cttccgcagt cagcgtattc
    28861 gcatgatttt tcaggatcct tcgacctcgt tgaatccccg tcaacgtatc tcgcaaattc
    28921 tggattttcc actgcgcctg aacaccgacc tggaaccgga acagcgtcgc aaacagatta
    28981 ttgaaacgat gcgtatggtt gggctgctac cggatcacgt cagttactat ccgcatatgt
    29041 tggcacccgg acaaaaacag cgtctgggtc tggcgcgcgc gctaatattg cgcccaaaag
    29101 tcattattgc cgatgaagcc ctcgcttcac tggatatgtc gatgcgttcg cagttgatta
    29161 atctgatgct ggagttacag gaaaaacagg gcatttcgta tatttatgtt acccagcata
    29221 tcggaatgat gaagcacatt agcgaccagg tgctggtgat gcatcagggc gaggttgtcg
    29281 agcgaggcag caccgcggat gtgctggcat cgccgctgca tgaactcacc aaacggctga
    29341 ttgccgggca ctttggcgaa gcattgacag cggatgcgtg gcgtaaagat cgctaatttt
    29401 ctgaatcctc cttccagatt atccacctca ttctgacaag agattaaatc ttgtcagaat
    29461 gaggtgatgg ataaaataaa atcaaatgcg cgtgatttaa gacgcaatct cactttgcag
    29521 gaacaaaagc tctggcgata tcttcgcagc cgacgttttg gtgatttcaa atttcgccgt
    29581 caacatccgg tggggagcta cattctcgat tttgcttgct gctcggcgcg cgtagtcgtt
    29641 gagctggatg gtgggcagca tgatttggca gttgcctatg ataccaggcg cactagctgg
    29701 cttgagtcgc agggctggac cgtgctgcgt ttctggaata acgagattga ttgtaatgag
    29761 gaggcggtgc cggagattat tctgcaggaa ctgaaccgcc gatcaccctc accctgaaag
    29821 ggcgaggggg ccgaccgagc cgaatagctg ttgtggtgaa aacatggaga cagtgctgga
    29881 gaatattcgg caaggtctga accatcccag ccatcgccat aaaagggtta ggggctgtat
    29941 gagcctgttt gttgctgggg taacaatatt tgcacaatat ggtcccctcg cccctctggg
    30001 gagagggtta gggtaagggg aaaagcgccc accctgccgc agcctgctcc ggtcggacct
    30061 ggcaactata gctactcaca gccaggttga ttataataac cgtttatctg ttcgtactgt
    30121 ttactaaaac gacgaatcgc ctgattttca ggcacaacaa gcatcaacaa taaggattaa
    30181 agctatgggt tttctttccg gtaagcgcat tctggtaacc ggtgttgcca gcaaactatc
    30241 catcgcctac ggtatcgctc aggcgatgca ccgcgaagga gctgaactgg cattcaccta
    30301 ccagaacgac aaactgaaag gccgcgtaga agaatttgcc gctcaattgg gttctgacat
    30361 cgttctgcag tgcgatgttg cagaagatgc cagcatcgac accatgttcg ctgaactggg
    30421 gaaagtttgg ccgaaatttg acggtttcgt acactctatt ggttttgcac ctggcgatca
    30481 gctggatggt gactatgtta acgccgttac ccgtgaaggc ttcaaaattg cccacgacat
    30541 cagctcctac agcttcgttg caatggcaaa agcttgccgc tccatgctga atccgggttc
    30601 tgccctgctg accctttcct accttggcgc tgagcgcgct atcccgaact acaacgttat
    30661 gggtctggca aaagcgtctc tggaagcgaa cgtgcgctat atggcgaacg cgatgggtcc
    30721 ggaaggtgtg cgtgttaacg ccatctctgc tggtccgatc cgtactctgg cggcttccgg
    30781 tatcaaagac ttccgcaaaa tgctggctca ttgcgaagcc gttaccccga ttcgccgtac
    30841 cgttactatt gaagatgtgg gtaactccgc ggcattcctg tgctccgatc tctctgccgg
    30901 tatctccggt gaagtggtcc acgttgacgg cggtttcagc atcgctgcaa tgaacgaact
    30961 cgaattgaaa taatcattct gttggtaaat attgggcggc gttctgccgc ccgttatctc
    31021 tgttatacct ttctgatatt tgttatcgcc gatccgtctt tctccccttc ccgccttgcg
    31081 tcaggataac gatttccttt acgaccaagg agcgcccatg gaacaacgcc acatcaccgg
    31141 caaaagccac tggtatcatg aaacgcaatc cagtactgcg gagtatgacg ttctgcctct
    31201 ggtcccggaa gccgcaaagg tcagcgatcc ctttctgctc gacgtgatcc ttgatgaaga
    31261 aacgctggct cccttccttt catggctggt ccctgcgcgc gttcttgcag tggaattgtt
    31321 ccctgaccag cttaccgtga cccgttcaca gaccttcacc gcttatgaac gcttgtctac
    31381 ggccctgacg gttgctcagg tttgcggcgt ccagcggtta tgtaactact attcggcgcg
    31441 acttacgccg ctccccgggc ctgattcctc cagggaaagc aatcataggt tggcacaaat
    31501 cacgcaatat gcccgccaac tggctagctc gccttctatt atcgacaacc gatcgcgcca
    31561 gcatctgaat gacgtcggtc ttactgcctg ggactgtgtg atcattaacc aaatcattgg
    31621 ttttattggc tttcaggcgc ggaccattgc gacatttcag gcttatctcg ggcacccggt
    31681 acgctggtta cccggtctgg aaatacaaaa ctacgccgac gcgtcactgt ttgctgatga
    31741 atcaatacgc tggcgaagca gctatgaggt ggaaaaacta cctgaagagt acacaaaaag
    31801 ttcaacagca gaactttgcc aactggccga aacactctct ctccacccta tttcactttc
    31861 ccttctcgaa aggttgttaa acagtacacg ggttaataca cagccggata ataagcttgc
    31921 ggcgttgtta tgcgcgcgga taaatggcag tcctgcttgt tttgccgcct gtatggattc
    31981 atcaaatgaa tataaaaaat cagccccctt ctgcgcaagg gcgaaaatga aattaaccaa
    32041 tgggctgacc gtcattctgt tgagcacgct accgttcagg cgatacaatg gctgacccga
    32101 gcacccgatc gctttagcgc cgcccagttc agccctttac tcgaacacga aaaatcatca
    32161 acgcagatta ttaatctgct ggtatggagc gggctgtgtg gctggataaa tcgcttaaaa
    32221 atcgcgttgg gtgagacata ttaaccttgc cgcgtcagac agattcgcgt aaaactgtca
    32281 gccgctctaa tggccaccaa aatagacaat tatgtttcag gacaacccgc tgctagcgca
    32341 gcttaaacag caactgcatt cccagacgcc acgcgctgaa ggggtggtaa aagccacaga
    32401 aaaaggcttt ggcttcctgg aagtcgacgc gcaaaaaagt tatttcattc cgccgccgca
    32461 gatgaaaaaa gtcatgcatg gcgaccgaat tatcgcggtg atccacagtg aaaaagaacg
    32521 tgaatccgca gagccagaag aactggttga accgttcctg actcgtttcg tgggtaaagt
    32581 tcagggcaaa aatgaccgtc tggccatcgt tcctgatcat ccactcttaa aagacgccat
    32641 tccttgccgc gcagcccgtg gcctgaacca cgagtttaaa gaaggcgact gggcggttgc
    32701 cgaaatgcgc cgtcatccgc tgaaaggcga tcgttctttc tatgctgaac tgacacaata
    32761 catcactttt ggtgacgacc actttgtacc gtggtgggtt acccttgcgc gccataatct
    32821 ggaaaaagaa gcaccagacg gcgtcgctac cgaaatgctc gatgaaggtc tggttcgtga
    32881 agatctgacc gcgctggatt ttgtcaccat cgacagtgcc agcacagaag atatggatga
    32941 cgcccttttc gctaaggcgt tgccggatga caaacttcag ctgattgtgg cgattgccga
    33001 tccaaccgcg tggattgctg aaggcagcaa gctggacaaa gccgcgaaaa ttcgcgcatt
    33061 caccaactat ctgcctggct tcaacatccc tatgcttcct cgcgagcttt ctgacgatct
    33121 ctgctcactg cgcgccaatg aagtccgccc ggtactggca tgccgcatga cgctctccgc
    33181 tgatggcacc attgaagata atatcgaatt ctttgccgcc accatcgaat ccaaagcgaa
    33241 gctggtgtat gaccaggttt ctgactggct ggaaaatacc ggtgactggc agcctgaaag
    33301 tgaagcaatt gccgaacaag tccgtttgct agcgcaaatt tgccaacgcc gcggcgagtg
    33361 gcgtcataac cacgcactgg tgtttaaaga tcgcccggat taccgcttta ttctcggtga
    33421 aaaaggtgaa gtgctggata tcgtcgccga gcctcgtcgc attgccaacc gtatcgtcga
    33481 agaagcgatg attgccgcta acatttgtgc ggcccgcgtt ctgcgcgata agctcggttt
    33541 tggcatctat aacgtgcata tgggctttga tccggcgaat gccgacgcgc tggcagcgtt
    33601 gctgaaaacg cacggtctgc atgtcgatgc cgaagaagtg ctcacgctgg acggtttctg
    33661 caaactgcgt cgtgaactgg atgcgcaacc aactggtttc ctcgacagcc gcattcgtcg
    33721 cttccagtca tttgctgaaa ttagcactga acccggtcct cactttggcc tcggtctgga
    33781 agcatacgcc acctggactt cgccgatccg taaatatggc gacatgatca accaccgtct
    33841 gctgaaagcg gttatcaaag gcgaaactgc gacgcgtccg caggatgaaa tcactgtcca
    33901 aatggccgaa cgtcgccgtc tcaaccggat ggcagaacgt gatgttggcg actggttata
    33961 cgcacgcttc ctgaaagata aagccgggac cggcacccgt ttcgcggcgg aaattgtcga
    34021 tatcagccgt ggcggcatgc gtgtgcgttt ggttgataac ggcgctatcg cctttattcc
    34081 ggcacctttc ttacacgctg tgcgcgatga actggtttgc agccaggaaa acggcactgt
    34141 acaaattaaa ggtgaaacgg tttacaaagt aactgacgtt attgacgtca ccattgccga
    34201 agtccgcatg gaaacccgcg gcattattgc gcgcccggtc gcgtaatctc ctttcacggc
    34261 ccattcctca tggatgggcc gtttatttcc ccgctctacc ttcgttatat ccaggcagat
    34321 tttttaactg actttcgttt gaaaactggc gtttttccaa aactggatta caaataaata
    34381 tataaaaaaa caatgcgttt agccgccgtt aatttcatgt aagtcatgtt gttcctttta
    34441 tttaaccgcg gactccgcag ttaaccggag gatatgcatc atgaaaaccg ttagggagtc
    34501 cacaacgttg tacaactttc tcggatcgca caatccatac tggcggttga cggaaagcag
    34561 cgatgttttg cgcttttcta ccaccgaaac cacagaacct gatcgtactt tgcagttatc
    34621 tgccgaacag gctgctcgca tcagggaaat gacggtcatc acctccagcc tgatgatgag
    34681 tctgaccgtc gatgagagcg atctttctgt gcatctggca ggacgaaaaa tcaataaacg
    34741 ggaatgggct ggcaacgcgt ctgcatggca tgacacaccg gcagttgctc gtgatttatc
    34801 acacgggctt tcctttgctg agcaggtagt ttctgaagca cattccgcaa tagtgattct
    34861 cgacagccgg gggaatatcc aacgcttcaa tcggttatgt gaagattaca ctgggttgaa
    34921 agaacacgac gtcattggcc aaagcgtgtt taaactgttt atgagccgtc gtgaagctgc
    34981 ggcatccagg cgcaataacc gtgtattttt tcgaagcggc aatgcatatg aagtcgaact
    35041 gtggatacca acacgtaaag gccagcggct gtttctgttt cgcaataaaa taaatttgtc
    35101 cacagcggca gtggcaaaaa cgagattttt ttaatctgtt ccggtaccga cattaccgaa
    35161 gagcgccgcg ctcaggagcg actgcgtatt ctggcaaata ccgacagtat caccggactg
    35221 ccgaatcgta acgcaatgca ggagttaatc gatcacgcta ttaatcaggc agataacaat
    35281 aaagttgggg ttgtgtatct tgatttggat aattttaaaa aggtcaacga cgcctatggg
    35341 catttgtttg gtgaccagtt attacgcgac gtgtcattgg ctattttaag ctgtctcgaa
    35401 catgaccagg tgttggcgcg tccaggtggc gatgagtttc tggtactggc atccaatacc
    35461 tcacaaagcg cgctggaagc aatggcatca cgaattttga cccgcttacg cctccccttt
    35521 cgtattggtt taattgaagt ttataccagc tgttcagtag gtatttcgct ctctcccgaa
    35581 catggttcgg acagcgcgac tattattcgt cacgccgaca cagcaatgta cacagcgaag
    35641 gaaggcggac gaggacaatt ttgcgttttt actccagaaa tgaatcagcg ggtatttgaa
    35701 tatctctggc tggataccaa cttgcgtaaa gcactggaaa acgatcagtt ggttattcac
    35761 tatcaaccga aaatcacctg gcgtggcgaa gtgcgcagtc tggaagcact agtacgttgg
    35821 cagtcacctg aacgtgggtt gattccaccg ttggacttca tttcctacgc cgaagagtca
    35881 gggctaattg tgcctttagg ccgttgggtg attctcgatg tcgtacgcca ggtggcaaag
    35941 tggcgggata aaggcataaa cctgcgagtg gcggtaaata tttctgcacg tcagctcgcc
    36001 gatcaaacca ttttcaccgc cctgaaacag gttctccagg aactcaattt tgaatactgc
    36061 cctatagatg ttgaactgac agagagttgt ctgattgaga atgatgaact ggcactgtct
    36121 gttattcaac aatttagcca actaggtgcg caagtgcatc tggatgattt tggtaccggc
    36181 tactcttcac tttcgcaact ggcgcgcttt ccgatcgatg ccatcaaact tgaccaggtt
    36241 tttgttcgag atattcacaa acaacctgtc tcgcagtcac tggtccgggc gatcgtcgct
    36301 gtggcccagg cattgaatct tcaggtgatc gccgaaggtg tagagagtgc aaaggaagat
    36361 gcttttttaa ccaagaacgg gatcaatgag cggcaaggat ttttgtttgc caaaccgatg
    36421 cccgccgtcg ccttcgaacg ctggtataaa cgctatctga agcgcgcata agtagatgaa
    36481 tatatatact tagcgctata tcccgtcatt atatatcgta ttcctggatg ataattagcc
    36541 gttgctgtta aacacttctc ttttagcggc aatttttgca ttattcattt tccacttttg
    36601 ctataaggat gtccttgatg tctgaatttg atgcccagcg cgttgccgaa cgtattgata
    36661 ttgtgttaga tatccttgtg gcaggcgatt accactctgc gatccataat ctcgagatac
    36721 ttaaagcaga actcttgcgc caggttgctg aatctacgcc agacatcccg aaggcaccgt
    36781 gggagattta acaatcgtgc cttcaggttt tcctatttga ggatgcccgg cgcgtcagta
    36841 tttttttatt tagtattata acgttataag aattacagcg atgaattccc gacaacaaac
    36901 tattctacag atggtcattg accagggtca ggttagcgta accgatctgg caaaagccac
    36961 tggagtttct gaagttacca ttcgccagga tctcaacacc ctcgaaaaac tgagttacct
    37021 ccgccgtgca catggctttg cagtttcgct tgatagtgat gacgtcgaaa cccgtatgat
    37081 gagcaactat acgctgaagc gtgaactcgc cgagtttgcc gcgtcactgg ttcaaccggg
    37141 cgaaaccatc tttatcgaaa atggcagcag caatgccctg ctcgcccgga ctctggggga
    37201 gcagaagaaa aatgtcacta tcatcacggt cagcagctac atcgcgcatt tgctgaaaga
    37261 cgcgccttgt gaagtcattt tgctcggtgg cgtgtaccag aaaaaaagcg aaagtatggt
    37321 tggcccgttg acacgccagt gcatccaaca ggtgcatttc agcaaagcat ttattggtat
    37381 tgatggctgg caacctgaaa ctggattcac cggtcgcgat atgatgcgta ccgatgtggt
    37441 caatgccgtg ctggaaaaag agtgcgaagc gatagtcctg actgacagct cgaaatttgg
    37501 tgctgtacat tcatactcca tcggtcccgt tgagcgattc aatcgcgtga ttaccgattc
    37561 gaaaatacgc gccagcgatc tgatgcatct tgagcacagt aaactcaccg ttcacgtcgt
    37621 tgacatttaa ccatttccct tctctacgga tgatttgcag tttggcaaat catccgctct
    37681 aagatgattc ctggttgata attaagacta tttacctgtt attaacacgc ttaagatata
    37741 aaattattat cagcgatata acaggaagtc attatcacct gcgtgatata accctgcgcg
    37801 cgagcagatt tcacggaata atttcaccag gcttattctt agctattata gttatagaga
    37861 gcttacttcc gtgaatcata aattcaggag agagtattat gtttgtaacg agcaaaaaaa
    37921 tgaccgcggc tgttctggca attactttgg caatgtctct gagtgcctgt tctaactggt
    37981 ctaaacggga ccgcaacacc gcaatcggcg cgggtgcagg ggcattaggc ggtgcagtac
    38041 tgaccgatgg cagtacgttg ggtacattag gtggtgcagc tgtcggtggt gttattggtc
    38101 accaggtcgg taaataatcg taaataaatg tcgattatcg actcgcaatt ataatacgct
    38161 tgattctgtt cagtacgaaa taactgaata atacgcacag taaaatattc gaaagccacg
    38221 gatatatccg tggcttttta tgttaaccgc ctgcgagttt taccttcatc cctttcgctt
    38281 caagtagtga ttttaataaa tcacgcttat cgccctggat ttcaataact ccatctttaa
    38341 ctgctccgcc gcagccgcat tttttcttca gttccgctgc aagttttgtc agttcggcat
    38401 catcgagatc gacaccggta atcaggcaaa cgcccttacc tttacgtcca ctggtctgac
    38461 gctgaatacg caccacaccg tcgcctttag ggcgtacagg ggccgctttg ggctcatcaa
    38521 tacgtccggt ttccgttgag tagaccagac ggctgttgga atcactcatc atgcactccg
    38581 ctgtaaagag gcgttgatcg ctttcagcgt ctgcgctgga tctaccgatt gcgttaccgg
    38641 gcgaccaatc accatataat caacaccagc cgacaacgcc tgttctggcg tcataatgcg
    38701 gcgctggtca ccagcttcac tcccctgcgg acgaatgccc ggcgtaacca gtttgaactc
    38761 ctgaccgaat acctgtttaa agcgcacagc ttcctgagca gaacacacca caccatcaag
    38821 gccacatttt tgcgtcagtg ccgccagacg ttctgcataa tctgcaggtg acagtgtcat
    38881 gccaagatcg accaggtcgc tggcttccat gctggtcaac actgtcacag caatcaaaag
    38941 cggtgcatct ttgccaaacg gaaccagtgc ctcacgcgct gcggtcatca tacgcgcccc
    39001 accagaggca tgaacattca ccatccacac gcctaagtca gctgcagcag cgacagcgtg
    39061 cgctgcagtg ttaggaatat cgtggaattt caggtcaaga aagatatcaa aaccacgctg
    39121 ttgaagttcg cgcacaaact gtggcccaaa caatgtaaac atctctttgc cgaccttcag
    39181 acgacaatcg cgtgggtcga tcttgtcgac aaaggacagc gcgtcatcac gattatgata
    39241 atcaagggca acaaccacag gagaattcgt aacagcgcgg gaagaagatg aagcagttaa
    39301 cgtcatgacc agaccttctt gatgatgggc accggaagtg gcgcgaaaca ggtaaacggc
    39361 gagcattcta cccgtggaaa taaaaaatta acagttgcga tttttcccct ggcaggcttt
    39421 ttctgctccg tcgttgccgg agcacaatgg aacatcaata actagtatgt tgtaactaaa
    39481 gtcggatttt tttaaaaatt acaggccatc aagaccgcga atcggtttaa tggttgacca
    39541 ggcccgacaa gacggacaat gccagtagag ggtgtatgcg gtaaaaccac atttctggca
    39601 gcgataacga ggcttactac gtaccttttc gccaaccatg tcacgcagca ccatcaggct
    39661 ctctttggca cgcccttctt ccgcttcatt taagtggtaa tccattaact tatggaacac
    39721 acgcatggtc ggatgacgct gaagctggcg cgtaatatag acctgtgcgg cctcactacc
    39781 gtcgcgcgct tcgatgatat cagcaagcat caattcagca tcggcaccgg tgttctcttc
    39841 caccgcgcgt tgcaggaatt ctgcccattc ggcagtttta cccaactgct gatagcaggt
    39901 ttgcaacatt tccagcgttt cgctgaccag ttctctgtcc tgggatatca cgcgttgcaa
    39961 actttcgacg gctttggcgt attctccttt cgccataaac acgcgtccca tcatgatgga
    40021 tacgcgggcg ctgtttttat ctgccgccgc cccttttttt agcaaggtca tggcacgatc
    40081 gagatcgtcg ctggccatat gctgcagggc taactcacag tagaaatggg caatttcgac
    40141 gcgctgttta tctttaccca gcttcaccag gcgttcggca acatcaattg ctttctgcca
    40201 ctcgctggta gcctggtaga tttgtagcaa ctgttgcagc gcgccaatgc ggaagtcagt
    40261 ttcatcggtc agctgattga acatgtcttc cgcgcggtcg tataacccgg cggccatgta
    40321 atcacgcccc agttgttgaa tcgccaacag acgctgttca taggtcagtg aggcgctttc
    40381 cattagggtc tgatggatgc gaatagcgcg atcaacttcg ccacgcgaac ggaacaggtt
    40441 tccgagcgta aggtgggctt caacggtgcc tgtatcctct ttaagcatat cgagaaacag
    40501 gtctaccgct ttatcctgtt gattactaag caggaagtta acccccgcta cgtaatcacg
    40561 cgacaagcgg ttggcttcat cttgcttgtt ttgttgcgca cttctgcggc ccatatacca
    40621 gccataggca gcggctacag gcaaaagcag aaacaacaac tccagcatag aaagttattc
    40681 cttcgccgct gacgagtgcg gcactacagc cacgtcagtc gcgggtgaaa gctggttttc
    40741 cagtcgcttt attttacgtt cagcgcgcgc cagggaaaca cgaactcgca gccagaacag
    40801 gccacaaatc aaccaaccga tagcaacccc cgcagcaaac aataccgcca gcaatgtgga
    40861 aatacggtac tccccttgcg ctaacagata attaaacgtc acctgttgat cgttctgcgc
    40921 acccaacgtg accgaaatca cgaagatcgc taacaccagt aagaaaatga gtaaatattt
    40981 cacattactt cccgttatgt ggttaaagcg aataaattat ggtcaactta ccgcaatagc
    41041 cctttaaagt accattttcg cgccgtctgc gaaatggcat ccacgcgcat tgttgatgat
    41101 ttaagggcta aattcggaaa atcaaccagc gttaactttc ttgttctcgt tgcgctattt
    41161 cgcgattttc ttccgcaggt ggtgttaatg gcccacaaat tcgttgcgca agccacgttg
    41221 ccaccgccac cagcgcccac gaaatcaacg tagctactac cagatcgcgt ggccaatgca
    41281 tcccgagcag caggcggctt cccatgactc ccgttgccca gaccagcaag atagcaatgg
    41341 ttaacgttcg ccgacgcggc cacagcaaac caacggccag cagtgcccaa ctggcagcaa
    41401 acatcgtgtg accggaagga aaggcaaacc ccgtctcttt ctgccagtgt gaacgcaaat
    41461 attgtgggat atttttctct tcagccaact gttctttcac tagatttccg cgttctgctc
    41521 gctttaaagt gtagaactca tcaaccggaa tatgatgtgt tttttccagc cagataacaa
    41581 aaggtcgtgg ttcctggact ttgtctttga tccaggattt aacgccttgt cccacaatga
    41641 ttacggccgc cagaatggca aataatacaa aggcagcctt aatgcgaaaa cgcagacacc
    41701 agagaaacca gccgaataaa atcaaatgtg taatgacgcc ccagggctgg gtgacagttt
    41761 cagtaaccca aaaagccgct tttagtagcc aactttgttc tccaggttgc caacgccagc
    41821 cagaaatcca tacggctact ggcatgacaa gcaatagtgc agctcccact gcggtacgtc
    41881 tggcaatcga acgcatggcc tctccttttt gataagtccc acaatcataa ctgaaaacgc
    41941 cagttccagg aaaaattgac agatttgtgc cattccgtga acgatcgacg cgtcgtgatt
    42001 aggtgaaccc cttctcgtta tggcaaaata agccaataca gaaccagcat tatctggaga
    42061 atttcatgca gcttaaacgt gtggcagaag ccaaactgcc aaccccatgg ggcgatttcc
    42121 tgatggtggg atttgaagaa ctggcaaccg gacacgatca tgtcgcgcta gtctatggcg
    42181 atatttccgg gcataccccg gtacttgcgc gcgtccattc cgaatgtctg accggtgacg
    42241 ccctgttcag cttgcgctgc gattgtggct tccagctcga agcggcattg acgcaaattg
    42301 ccgaggaagg ccgtggtatt ttgctgtatc accgtcagga aggtcgtaac attggtctgc
    42361 tgaataaaat ccgcgcttac gcactgcagg atcaaggtta cgataccgta gaggctaacc
    42421 accagttagg cttcgccgct gatgagcgcg acttcactct ttgcgctgat atgttcaaac
    42481 tccttggcgt caatgaagtc cgcttgttaa ccaataaccc gaaaaaagtc gaaattctga
    42541 ccgaagcagg gattaatatt gttgaacgcg taccattgat tgtaggtcgt aaccccaata
    42601 acgaacatta tctcgatacc aaagccgaga aaatgggtca tttgctgaac aaataaccct
    42661 ctcgcattgt ggaattaatt tgcttgccgg aagcaaaata accggcaagc aaatatttgt
    42721 tacttcagca tattacgaat gacataatgc aaaatgccgt cgttctggta gtaggtcaac
    42781 tccgtcgcgg tgtcgatacg acaacggcag ggtacgacct cctggctacc atccgcgcgc
    42841 gtaagcgtca ccggaaccgt cgcaccgggt tgtagatttt gcagatcgcc aatatcaatc
    42901 ttctcttccc cggttagtcc taacgtttta cgcgttacgc cttgcggaaa ttccagcggc
    42961 aggatgccca tgccaattaa attcgaacgg tgaattcgtt caaacgattc ggcaatcacc
    43021 acacgaattc caagcagacg aggacctttt gccgcccagt cacggcttga gcctgaacca
    43081 tactctttcc cggcaatcac cgccagcggc gtttgttcct gcttatagcg catcgcagca
    43141 tcataaatag agactacgtc gctgtcaggt aaatgccgcg tcatcccccc ttcaacgcca
    43201 ggcaccattt cattacggat gcgaatattg gcgaaggtgc cgcgcatcat cacttcatgg
    43261 ttaccacgcc gcgaaccgta ggagttaaag tcttttcgct caacaccccg accttgtaga
    43321 tatcgacccg ctgggctgtc gggcttaata ctgcccgccg gagagatatg gtcagtggtg
    43381 actgaatccc ccagcattgc gaggatccgc gcaccgtgaa tatcttccac tggtgctggt
    43441 gttgcctgca tttcatcaaa gaaaggcgat aagcgaatat aggttgagtc ctcctgccaa
    43501 ccgtaggtat cggatcgtgt gacgttaatt tccttccact ctgctgtgcc ttcaaaaact
    43561 tctgcgtact ctttgcggaa catttctgtg gagacttgtt ctaccgcacg ggcaatttct
    43621 tgtgccgatg gccagatatc tttcagataa accggatcgc ctttacgatc atgaccaata
    43681 ggctcagaag ccaggttgat attcatattt cccgccagcg cataggcaac caccagcggc
    43741 ggcgaggcca gccagttggt tttaaccagc ggatggatac ggccttcaaa gttacggttg
    43801 ccggacagca ctgcaccgac ggttaaatcg ccttttttga ttgccgtttc gataggatcg
    43861 ggcagcggcc cagagttacc aatacaggta gtacaaccgt atcccacaag gttaaacccc
    43921 agttcgtcga gaaacggtgt cagtttcgct tttgccagat agtcagaaac aactttcgag
    43981 cctggtgcca gcgacgcttt gacccacggt tgccgcttga ggccaagaat tacggctttt
    44041 ttcgccagca agcctgcagc catcagcaca cttgggttag aggtgttggt gcacgaggtt
    44101 atcgcagcaa tgaccacagc gccatcaggt aactgatact gatgtccgtt cataacataa
    44161 tcgaccggct ggcgatcttt atgcgtggca ttcacttcca gttcgttact ggcggcaaat
    44221 acttttggta catcgggcag tgcaacgcga tcctgtgggc gttttggccc tgccaggctc
    44281 gcttcaacgt cattcatatc cagttctaac gtactggtaa aaatcggttc atcgcccggg
    44341 ttacgccaca tgccctgcgc tttggcatat ttttcgacca actcaacctg atcttcgctg
    44401 cgcccgctta aacgcatgta atcgagggtt acagcatcga ttgggaagaa gccacaggtg
    44461 gcaccatatt ctggcgacat attggcaatg gtggcgcgat ccgccagcgg tagtgaatcc
    44521 agaccatcac cataaaattc gacgaatttc tccaccacgc catgtttgcg cagcatttgg
    44581 gtaacagtga gaaccaggtc tgtggcggta ataccttcac gtaatttacc ggtaagttta
    44641 aagcccacta cgtccgggat aagcatggaa accggctggc ctaacattgc ggcttctgct
    44701 tcgatcccac ccacgcccca ccccagcacg ccaaggccgt tgatcatagt ggtgtgcgag
    44761 tcagtaccaa cgagtgtatc cggataagca atccattcac cgtcctgcaa ttcactccac
    44821 actgctttgc cgagatattc gaggttaacc tgatggcaaa tgcctgtgcc tggcggcacg
    44881 acgctaaacc gactgaacgc ttgctttccc catttcagga acacataacg ttcgtggttg
    44941 cgctccattt ccaggcgtac gttttcttca aatgcctcat catcaccaaa acgatcgacg
    45001 gtcaccgagt ggtcaatgac caggtcgacc ggtgagagcg ggttaacctt tgcagtatcg
    45061 ccgccgaggc gtttaaccgc ttcgcgcatt gccgccagat caacaacggc aggtacgccg
    45121 gtaaagtcct gcatcaacac ccttgccggg cggtaggcaa tttcacggtc agcatgggca
    45181 tttttcagcc atcctgccag cgcgtggata tcctcttcgg taaccgagtt accatcctgc
    45241 cagcgcagca ggttttcgag caaaactttg agtgacttgg gtagacgggt gatatcgccc
    45301 agtgatttag cagcaagcgg caggctgtag tagtggtaag ttttatcttt ggcctgcaac
    45361 gtgtccttac tggcttctcg tagggttgac gacatagctc ctccttaatg acagggttgc
    45421 gaagccctga ttctcttcag ggtgaatatt aaagataaca caaacatcgc gtaacgattt
    45481 gataacaacc caaattgata aaagaggaga aaccttgtgg caaagcaaat gacaaccccg
    45541 ccgcagcggg gtcaaggatc agtgagtaat gatccagagg agttcggccc agaagagcat
    45601 cgacagagaa aacgcgccca tccaggacca atatttcata tttgtgttga tcataggcta
    45661 cacccttaac ggttattatt ttgtgttgag ataactcaag ctgttattgc tgcggtgtat
    45721 taaaagccct ttaattcatt aaaaatgggt aataagaatg atgataatta ttgtttcggc
    45781 tgatcatctt cagcaaacag ttctataaag gcttgctgct gcgatgttag ttgccagtcg
    45841 tctggaataa cgacggaaca ttcccgtttc gtttctgttt cattgtcact atgctggcat
    45901 aatggtgacg acttcagttg ctcctgaccg accatagtta tccccacact ttccaaatca
    45961 gcagtgcgac aaccacccag aaaagtgcag aaccaagaaa aaccgccagc caggctttac
    46021 gttttaattc aggatcgcgg cggggttcct gatttccaga aggcattgct aacctcatat
    46081 aatcgccatc acttatcagc aaggcgttga acgattggtg taattaatca tacgatgaga
    46141 caaatcctaa agaaacttta gt
//
LOCUS       DAKISA010000024        43121 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.024, whole genome shotgun sequence.
ACCESSION   DAKISA010000024 DAKISA010000000
VERSION     DAKISA010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43121
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.024"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..1112)
                     /gene="setA"
                     /locus_tag="OVW45_001377"
     CDS             complement(<1..1112)
                     /gene="setA"
                     /locus_tag="OVW45_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCV5525872.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAV"
     gene            complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="OVW45_001378"
     CDS             complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="OVW45_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCV5525873.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            1434..3089
                     /gene="sgrR"
                     /locus_tag="OVW45_001379"
     CDS             1434..3089
                     /gene="sgrR"
                     /locus_tag="OVW45_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCV5525874.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      3123..3222
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3253..4236
                     /gene="thiB"
                     /locus_tag="OVW45_001380"
     CDS             3253..4236
                     /gene="thiB"
                     /locus_tag="OVW45_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCV5525875.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVYPAFQNAIP
                     TGNWMYPVANVTQPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            4212..5822
                     /gene="thiP"
                     /locus_tag="OVW45_001381"
     CDS             4212..5822
                     /gene="thiP"
                     /locus_tag="OVW45_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCV5525876.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMMCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQSVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     NVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            5806..6504
                     /gene="thiQ"
                     /locus_tag="OVW45_001382"
     CDS             5806..6504
                     /gene="thiQ"
                     /locus_tag="OVW45_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCV5525877.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGITG"
     gene            complement(6574..7338)
                     /locus_tag="OVW45_001383"
     CDS             complement(6574..7338)
                     /locus_tag="OVW45_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCV5525878.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWMAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(7424..8302)
                     /gene="araC"
                     /locus_tag="OVW45_001384"
     CDS             complement(7424..8302)
                     /gene="araC"
                     /locus_tag="OVW45_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCV5525879.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPPIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            8641..10341
                     /gene="araB"
                     /locus_tag="OVW45_001385"
     CDS             8641..10341
                     /gene="araB"
                     /locus_tag="OVW45_001385"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCV5525880.1"
                     /translation="MAIAIGLDFGSDSVRALAVECATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKIVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATNAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            10352..11854
                     /gene="araA"
                     /locus_tag="OVW45_001386"
     CDS             10352..11854
                     /gene="araA"
                     /locus_tag="OVW45_001386"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCV5525881.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            12023..12718
                     /gene="araD"
                     /locus_tag="OVW45_001387"
     CDS             12023..12718
                     /gene="araD"
                     /locus_tag="OVW45_001387"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCV5525882.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            12793..15144
                     /gene="polB"
                     /locus_tag="OVW45_001388"
     CDS             12793..15144
                     /gene="polB"
                     /locus_tag="OVW45_001388"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCV5525883.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHTSPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            15308..18214
                     /gene="rapA"
                     /locus_tag="OVW45_001389"
     CDS             15308..18214
                     /gene="rapA"
                     /locus_tag="OVW45_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCV5525884.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPVQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            18226..18885
                     /gene="rluA"
                     /locus_tag="OVW45_001390"
     CDS             18226..18885
                     /gene="rluA"
                     /locus_tag="OVW45_001390"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCV5525885.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWE
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="OVW45_001391"
     CDS             complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="OVW45_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCV5525886.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            20072..22426
                     /gene="lptD"
                     /locus_tag="OVW45_001392"
     CDS             20072..22426
                     /gene="lptD"
                     /locus_tag="OVW45_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCV5525887.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            22479..23765
                     /gene="surA"
                     /locus_tag="OVW45_001393"
     CDS             22479..23765
                     /gene="surA"
                     /locus_tag="OVW45_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCV5525888.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            23765..24754
                     /gene="pdxA"
                     /locus_tag="OVW45_001394"
     CDS             23765..24754
                     /gene="pdxA"
                     /locus_tag="OVW45_001394"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCV5525889.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            24751..25572
                     /gene="rsmA"
                     /locus_tag="OVW45_001395"
     CDS             24751..25572
                     /gene="rsmA"
                     /locus_tag="OVW45_001395"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCV5525890.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            25575..25952
                     /gene="apaG"
                     /locus_tag="OVW45_001396"
     CDS             25575..25952
                     /gene="apaG"
                     /locus_tag="OVW45_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCV5525891.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            25959..26801
                     /gene="apaH"
                     /locus_tag="OVW45_001397"
     CDS             25959..26801
                     /gene="apaH"
                     /locus_tag="OVW45_001397"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCV5525892.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(26879..27358)
                     /gene="folA"
                     /locus_tag="OVW45_001398"
     CDS             complement(26879..27358)
                     /gene="folA"
                     /locus_tag="OVW45_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCV5525893.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(27550..29412)
                     /gene="kefC"
                     /locus_tag="OVW45_001399"
     CDS             complement(27550..29412)
                     /gene="kefC"
                     /locus_tag="OVW45_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCV5525894.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGTAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(29405..29935)
                     /gene="kefF"
                     /locus_tag="OVW45_001400"
     CDS             complement(29405..29935)
                     /gene="kefF"
                     /locus_tag="OVW45_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCV5525895.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(30043..31374)
                     /locus_tag="OVW45_001401"
                     /pseudo
     CDS             complement(30043..31374)
                     /locus_tag="OVW45_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(31433..31720)
                     /gene="fixX"
                     /locus_tag="OVW45_001402"
     CDS             complement(31433..31720)
                     /gene="fixX"
                     /locus_tag="OVW45_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCV5525896.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTYRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(31717..33003)
                     /locus_tag="OVW45_001403"
     CDS             complement(31717..33003)
                     /locus_tag="OVW45_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCV5525897.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(33054..33995)
                     /locus_tag="OVW45_001404"
     CDS             complement(33054..33995)
                     /locus_tag="OVW45_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCV5525898.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(34010..34780)
                     /locus_tag="OVW45_001405"
     CDS             complement(34010..34780)
                     /locus_tag="OVW45_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCV5525899.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            35253..36767
                     /gene="caiT"
                     /locus_tag="OVW45_001406"
     CDS             35253..36767
                     /gene="caiT"
                     /locus_tag="OVW45_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCV5525900.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            36798..37940
                     /gene="caiA"
                     /locus_tag="OVW45_001407"
     CDS             36798..37940
                     /gene="caiA"
                     /locus_tag="OVW45_001407"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCV5525901.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            38069..39286
                     /gene="caiB"
                     /locus_tag="OVW45_001408"
                     /pseudo
     CDS             38069..39286
                     /gene="caiB"
                     /locus_tag="OVW45_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
     gene            39360..40913
                     /gene="caiC"
                     /locus_tag="OVW45_001409"
     CDS             39360..40913
                     /gene="caiC"
                     /locus_tag="OVW45_001409"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCV5525902.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTTNLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATVTECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            41022..41807
                     /gene="caiD"
                     /locus_tag="OVW45_001410"
     CDS             41022..41807
                     /gene="caiD"
                     /locus_tag="OVW45_001410"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCV5525903.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            41813..42403
                     /gene="caiE"
                     /locus_tag="OVW45_001411"
     CDS             41813..42403
                     /gene="caiE"
                     /locus_tag="OVW45_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCV5525904.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(42612..43007)
                     /gene="caiF"
                     /locus_tag="OVW45_001412"
     CDS             complement(42612..43007)
                     /gene="caiF"
                     /locus_tag="OVW45_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCV5525905.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCAVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
BASE COUNT        10343 a        11740 c        11342 g         9696 t
ORIGIN      
        1 acagcaaagt gcccccaact ttgtgcaatt gctccctgaa taacgccagc cagaattacc
       61 ccggtagaaa tactgttagt aaataaggtg gtagctgccc ccgctcttcc aggcattaaa
      121 tcctgaaacc atagcatccc aatacccgca acaatgccga taaatacagc gttaaaaagt
      181 tgcagcgtca tcaacgccat acggctatga aagaaaatca atccggtgta aaacagtact
      241 cctgccgcca ctgctatgac catcattcgc cgcttaccat aacgtttgac atagtagcca
      301 gccagaatca ttgctggtat ttccagtcca gctgccgtcc ccatcaggaa acccgccagt
      361 ttgtctggca atcctaactc gctactgatc cacaacggca tatcaataat gtacatggtg
      421 ttgcaggtcc acattaacgt cgaggcgaca aataacatcc gtacgttact atcctgccag
      481 ccaccttgca ttgataaagc attttccgac ggcagttcta cccgcgccac agacggaagc
      541 ataaatgcaa tcaataccag actgagtgtg aatatcccgg cggcaatcga aaacatcacc
      601 gtaaagccgt aattcaacgc cagcataaag gccaacggtg gaccgataac ccatgccaga
      661 gaaagctgcg cacgcatcac cgagctaaac atcaccactt ctcgcgccga gttatccgca
      721 tattcccgcg ccagagcaaa taactgtggc attgccgtat tggccagaga tgccagaagc
      781 acaccacagg tgataagcgt cagataatga cgattaaatg caaacaatag cgcattgccg
      841 atagccatca aacagcaaaa tataatcagt tttcgccgat cgccctgact gtcagaacgt
      901 tttgccaacc agaggcttac gccgatccca gcaatagcat tcaccgtata aaagagtccg
      961 atccagaaag gttgcgcgcc aacctcacga ctcagaaata agctcaatgt aggagcctgt
     1021 agcgccccgg ccacccccat cataaaagcg accagcataa atgccgcgta aacaccgttc
     1081 atacggcgag ccatcgtcat tatccagatc atacgttccc tttttagcgc ggcgagaata
     1141 aaaaaaacca gcaggtataa tctgctggcg ggtgatttta caccaatact cagtcacaca
     1201 tgatgcaggc aagtcaactt tcagaattgc ggtcatccca ctgcatcagt ccttcaagca
     1261 tggttaatcg ttgtgggacg cttaaccaac gcaaccagca caacttcgct gtcgcggtaa
     1321 aatagtgctg ataaaactga cgcatggggc acctccttgc ttcatcgtta ggaattattg
     1381 acttaatata gggaaaataa aattgctgtc ttttgcgcag gagttcccct tttatgccat
     1441 ctgctcgtct gcaacaacag ttcatccgcc tgtggcaatg ctgcgagggt aaatcgcagg
     1501 acacaacgct caacgaactg gcagcgttat tgagctgctc gcgtcgtcat atgcgtaccc
     1561 tgctcaacac catgcaggat cgcggctggc tgacgtggga agcggaagtc gggcgcggta
     1621 aacgctcgcg tctgacattc ctctataccg ggctggcgct tcagcaacag cgggcggaag
     1681 acctgctgga gcaggatcgt atcgaccaac tagtgcagtt ggttggcgac aaagcgactg
     1741 tgcggcaaat gctggtttct catctgggcc gcagcttccg ccaggggcgg cacatcctgc
     1801 gcgtgctcta ctatcgtccg ttgcgtaatc tgctacctgg cagcgcattg cgccgttccg
     1861 aaacccatat tgcccggcaa atcttcagtt ccctaacgcg cataaatgag gaaaatgggg
     1921 aactggaagc agacatcgcc caccactggc agcaaatatc accgcttcac tggcgtttct
     1981 ttttgcgtcc aggagtccat tttcatcatg gtcgtgaact ggaaatggac gacgtgatcg
     2041 cctctttaaa acgaatcaat acgctgccgc tctattcgca tattgcggac attgtgtcgc
     2101 cgacgccctg gacgctggat atccatctca cgcaaccgga ccgctggtta ccgttactgc
     2161 tggggcaagt tccggcgatg atcctgccgc gcgaatggga aaccctcagt aactttgcca
     2221 gccatcccat cggcaccggt ccgtatgcgg tgatccgcaa caccaccaat caactgaaaa
     2281 ttcaggcatt cgatgacttc ttcggttacc gggcattaat cgatgaagtt aacgtctggg
     2341 ttctgccgga aattgccgac gagccagctg gaggtctgat gctaaaaggt ccacagggtg
     2401 aggaaaaaga gattgaaagc cgcctggagg aaggttgcta ctatttacta ttcgacagcc
     2461 gcacccatcg cggggcgaat cagcaagtca gggactgggt aagctatgtg ctttctccaa
     2521 ctaatctggt ctatttcgct gaggaacagt accagcaact gtggttcccg gcttatggac
     2581 tgctcccccg ttggcaccat gcccgcacca taaagagcga aaaaccggct ggcctggaaa
     2641 gcctcaccct gaccttttat caggatcaca gtgagcatcg ggtgattgcc gggatcatgc
     2701 agcagattct ggcaagtcac caggtcacgc tggaaatcaa agagatcagc tacgatcagt
     2761 ggcatgaagg agagatcgag agcgatatct ggcttaacag cgccaacttt acgctgccgc
     2821 tggatttttc gctgttcgcg cacttgtgcg aggtgccgct gctccaacac tgtattccga
     2881 tcgactggca agccgacgcc gcccgctggc gcaatggcga aatgaacctg gcgaactggt
     2941 gccagcaact ggtcgccagc aaagcaatgg tgccgcttat ccaccactgg ctgatcattc
     3001 aggggcaacg cagtatgcgc ggcctgcgca tgaacaccct cggctggttc gattttaaat
     3061 cagcgtggtt tgcgccaccg gatccatgat tgctggtaga taacaaaatc actacactaa
     3121 cgccgttctc aacggggtgc cacgcaaacg cgtgcgctga gaaaataccc gtcgaacctg
     3181 atccggataa cgccgacgaa gggatttgag gctccttctc aagtcctttg ccactctttt
     3241 ttgaggtgca aagtgttaaa aaaatgtctt cccctgctgt tgctgtgcac agcgcccgtt
     3301 ttcgctaaac ccgttctgac tgtttatacc tacgattcct tcgccgccga ctgggggcct
     3361 ggtccaaaga tcaaaaaagc ttttgaagcc gactgtaact gcgaactgaa actggtggcg
     3421 ctggaagatg gcgtttcgct tctcaaccgt ctacggatgg aaggcaaaaa tagtaaagcc
     3481 gatgtggtgc tggggctgga taacaatctg ttagacgccg ccagtaaaac cggactattt
     3541 gccaaaagcg gtgtggcagc ggatgccgtt aacgttcccg gcggctggaa taatgacact
     3601 ttcgtcccgt ttgattacgg ctatttcgcc ttcgtctatg acaagaacaa actgaaaaac
     3661 ccgccgcaaa gcctgaaaga attggttgag agcgatcaga actggcgggt gatttatcag
     3721 gatccgcgta ccagcacgcc ggggctgggt ctgctgttgt ggatgcaaaa agtctatggc
     3781 gatgacgccc cacaagcctg gcagaaactg gcgaagaaaa ctgtcacggt caccaaaggc
     3841 tggagcgaag cctatggcct gtttttaaaa ggcgaaagcg atctggtact gagttacacc
     3901 acctctccgg cttatcacat tctcgaagag aagaaagata actacgccgc cgcgaacttc
     3961 agcgaaggtc actatctgca ggtggaagtc gccgcccgca ccgctgccag caagcagccg
     4021 gagctggcgc aaaaattcct ccagtttatg gtttatccgg cttttcagaa tgcgatccca
     4081 accggcaact ggatgtatcc ggtggcaaac gtcacgcagc ctgccggttt tgaacaattg
     4141 accaaaccag caaccacgct ggagttcacg ccagccgaag tggcggcaca acgtcaggca
     4201 tggattagcg aatggcaacg cgccgtcagc cgttaattcc cggctggtta attccaggtg
     4261 taagcgccgc cacgctggtg gtagcggttg cgctcgcggc gtttctcgcc ctgtggtgga
     4321 acgcgccgca gggtaactgg gtagcagtct ggcaggacag ctacctgtgg catgtggtgc
     4381 gcttctcctt ctggcaggcg tttctctcgg cgctactctc tgtcgtaccc gcgatattcc
     4441 tcgcccgcgc cctctatcgc aggcgctttc cgggtcggct ggcgctgttg cgtctgtgcg
     4501 caatgacctt gatcctcccg gtgctggttg ctgttttcgg cattcttagc gtctatggtc
     4561 gtcagggctg gctggcatcg ctctgccaat cgctcggtct ggagtggacc ttttcgccct
     4621 acggcctgca aggcattttg ctggcccatg tgttttttaa tctgccgatg gcgagccgtt
     4681 tattacttca ggcactggaa aacattcccg gcgaacaacg tcaacttgcc gcccagcttg
     4741 gaatgcgtgg ctggcacttc tttcgtttcg tcgaatggcc gtggttacgg cgacaaatcc
     4801 cgccagttgc tgcgcttatc tttatgctct gtttcgccag cttcgccacc gtactgtcgc
     4861 taggcggcgg tccgcaagcg accactatcg agctggctat ttatcaggcg ctgagttacg
     4921 actacgatcc tgcccgcgcg gcgatgctgg cgctgatcca gatgatgtgt tgccttgggc
     4981 tggtgctgtt gagtcagcga ttgagtaagg ccattgcgcc cggcaccacg ctgctgcaag
     5041 gctggcgcga cccggacgat cgtctgcata gccgcatttg cgacacggtg ttaatagtgc
     5101 tggcgctgct gctgttgttg ccaccgttgc tggcggtgat cgtcgatggg gtaaatcgcc
     5161 agttgccgga agtgctggca caatcggtgc tgtggcaggc gctgtggacc tcgttgcgta
     5221 ttgcgctggc ggcaggtgta ttgtgcgtag tgctgaccat gatgctgcta tggagcagtc
     5281 gcgaacttcg ggcgcggcag aaaatgctgg cgggtcaggc gctggagatg agcggcatgt
     5341 tgatcctcgc catgccgggg attgtgctgg ctaccggctt ctttttactg ctcaacaaca
     5401 ctatcggcct gccacaatct gctgacggca ttgtgatttt caccaatgcg ttaatggcga
     5461 tcccttacgc gctgaaagta ctggaaaacc cgatgcgcga tatcaccgcc cgctacagca
     5521 tgttgtgtca gtcgttgggc attgaaggct ggtcacgctt aaacgtggtg gagctgcgcg
     5581 ccctgaaacg cccactggcg caggcgctgg cctttgcatg cgtgctgtcg attggtgatt
     5641 ttggcgtggt ggcgttgttc ggtaacgatg atttccgcac cctgccgttt tatctctacc
     5701 agcaaattgg ctcctatcgc agccaggacg gcgcggtcac cgcgttaatt ctgctactac
     5761 tctgttttct gctgtttacc gtgattgaaa aactaccggg gcgaaatgtt aaaactgact
     5821 gatatcacct ggctttacca ccatttgccg atgcgtttta gcttaacggt agaacgcggc
     5881 gagcaggtgg cgatcctcgg gccaagcggc gcaggtaaaa gtaccctgct gaatttgatt
     5941 gccggttttc tgacgccagc cagcggttcg ctgactatcg acagcgttga tcacaccacg
     6001 acgccgccgt cacgccgtcc ggtatcgatg ctgtttcagg agaacaacct gttcagccac
     6061 ctgacggtcg cgcagaacat cgggctgggg cttaatccgg gattgaagct gaacgcggcg
     6121 cagcaggaga aaatgcacgc tatcgcccgc cagatgggga ttgataattt aatggcgcgg
     6181 ttaccgggcg agctttccgg cggtcagcga cagcgcgtgg cgttagcgcg ttgtctggta
     6241 cgcgagcagc cgattttatt gctcgatgaa ccgttctctg cgctcgatcc ggcgctacgc
     6301 caggagatgt tgacgctggt gagcacgagc tgccagcagc aaaaaatgac gttattgatg
     6361 gtgtcgcaca gtgtggaaga tgccgcgcgg atcgccacgc gctcggtggt ggtcgccgac
     6421 gggcgcatcg cctggcaggg taaaaccgat gaattgttaa gcggtaaggc gagtgcttcg
     6481 gcgctgttgg gaattacagg ttaaaagcgc acggacagtc ccctcgcccc tctggggaga
     6541 agggaacaaa ccagcgccga tgcaaactat cccctaaacc ccaaccactt tacgcaaaat
     6601 atcgatatac accggcatca acgggtggcg aattaacacc accagcgcca ccacgccgat
     6661 ggcagaaatc aacggcgtca gccacaacaa acgaccgcgg gacaaataat gactcaaacg
     6721 gtcagtcgct ttaccactgc gccataaccg ccagcacagc cagccaccaa cccacaaaaa
     6781 taccgccgtc gccagcagca accatttaaa ctcaccgctc tgcatcccgg caggaatatc
     6841 gatcgccgcg cccgccagaa tccctggcag gaagtaaaac ggcggccaca gcaggcagcc
     6901 gataatattc ggcgtgataa attttgccac tggcagatcc agcattcccg ccaccattgg
     6961 caccagcgga cgcgtcgggc caacaaaacg accgaccaga atggtgaaca tgctgtgttg
     7021 atgcaacgca tgttcagttt tatcaagtag tgctttgttt ttcttcagaa atgaccagcg
     7081 atgcaacggc tttttaaaac gccagcccag ccagaaggag atccagtcgc ccagcaggca
     7141 accgacaatc cccgccatcc acgcgtgcca gaaacttaac tcgccgctgc caatcagcgc
     7201 tcccagcccc gccatcagca ccgtaccggg tagaatcaaa ccgaccagcg ccagcgactc
     7261 cagaaaggcc accaacacca ccgccatcaa tgaatacacg gtggattggg taataaagtg
     7321 ttccagcaat gcttgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc
     7381 tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg
     7441 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
     7501 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
     7561 tggcgatagg catccgggta gtgctcaaaa gcagcttcgc ctgactaatg cgttggtcct
     7621 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
     7681 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
     7741 cgctgatgta ctgacaagcc tcgcgtacac gattatccat cggtggatgg agcgactcgt
     7801 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
     7861 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
     7921 gctggtgcgc ttcatctggg cgaaagaaac ccgtattggc aaatattggc ggccagttaa
     7981 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
     8041 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
     8101 gtcggcagac aaattctcgc ccctgatttt tcaccacccc ctgaccgcga atggtgagat
     8161 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
     8221 cctcaatcgg cgttaaaccc gccaccagat gggcgttaaa cgagtatccc ggcagcaggg
     8281 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt
     8341 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaaccc
     8401 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
     8461 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg
     8521 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
     8581 gctttttttc gcaactctct actgtttctc catacccgtt tttttggatg gagtgaaacg
     8641 atggcgattg caattggcct cgattttggc agtgattctg tgcgagcttt ggcggtggaa
     8701 tgcgctaccg gtgaagagat cgccaccagc gtagagtggt atccccgttg gcagaaaggg
     8761 caattttgtg atgccccgaa taaccagttc cgccatcatc cgcgtgacta cattgagtca
     8821 atggaagcgg cgctgaaaat cgtgcttgca gagcttagcg ccgaacagcg cgcagctgtg
     8881 gtcgggattg gcgttgacac aaccggctcg acgcccgcac cgattgacgc cgacggtaac
     8941 gtgctggcgc tgcgcccaga gtttgccgaa aacccaaacg cgatgttcgt attgtggaaa
     9001 gaccacactg cggttgaaga agcggaagag attacccgtt tgtgccacgc gccgggcaac
     9061 gttgactact cccgctatat tggcggtatt tattccagcg aatggttctg ggcaaaaatc
     9121 ctgcatgtga ctcgccagga cagcgccgtg gcgcaatctg ccgcatcgtg gattgagctg
     9181 tgcgactggg tgccagctct gctttccggt accacccgcc cgcaggatat tcgtcgcgga
     9241 cgttgcagcg ccgggcataa atctctgtgg cacgaaagct ggggcggctt gccgccagcc
     9301 agtttctttg atgagctgga cccgatcctc aatcgccatt tgccttcccc gctgttcact
     9361 gacacctgga ctgccgatat tccggtgggc accttatgcc cggaatgggc gcagcgtctc
     9421 ggcctgcctg aaagcgtggt gatttccggc ggcgcgtttg actgccatat gggcgcagtt
     9481 ggcgcaggcg cacagcctaa cgcactggta aaagttatcg gtacttccac ctgcgacatt
     9541 ctgattgccg acaaacagag cgttggcgag cgggcagtta aaggtatttg cggtcaggtt
     9601 gatggcagcg tggtgcctgg atttatcggt ctggaagcag gccaatcggc gtttggtgat
     9661 atctacgcct ggtttggtcg cgtactcggc tggccgctgg aacagcttgc cgcccagcat
     9721 ccggaactga aagcgcaaat caacgccagc cagaaacaac tgcttccggc gctgaccgaa
     9781 gcatgggcca aaaatccgtc tctggatcac ctgccggtgg tgctcgactg gtttaacggt
     9841 cgtcgcacgc caaacgctaa ccaacgtctg aaaggggtga ttaccgatct taacctcgct
     9901 accaacgctc cgctgctgtt cggcggtttg attgctgcca ccgcctttgg cgcacgcgca
     9961 atcatggagt gctttaccga tcaggggatc gccgtcaata acgtgatggc gctgggcggc
    10021 atcgcgcgga aaaaccaggt cattatgcag gcctgctgcg acgtgctgaa tcgcccgctg
    10081 caaattgttg cctctgacca gtgctgtgcg ctcggtgcgg cgatttttgc tgccgtcgcc
    10141 gcgaaagtgc acgcagacat cccatcagcc cagcaaaaaa tggccagtgc ggtagagaaa
    10201 accctgcaac cgcgcagcga acaggcacaa cgctttgaac agctttatcg ccgctatcag
    10261 caatgggcga tgagcgccga acaacactat cttccaactt ccgccccggc acaggctgcc
    10321 caggccgttg cgactctata aggacacgat aatgacgatt tttgataatt atgaagtgtg
    10381 gtttgtcatt ggcagccagc atctgtatgg cccggaaacc ctgcgtcagg tcacccaaca
    10441 tgccgagcac gttgttaatg cgctgaatac ggaagcgaaa ctgccctgca aactggtgtt
    10501 gaaaccgctg ggcaccacgc cggatgaaat caccgctatt tgccgcgacg cgaattacga
    10561 cgatcgttgc gctggtctgg tggtgtggct gcacaccttc tccccggcca aaatgtggat
    10621 caacggcctg accatgctca acaaaccgtt gctgcaattc cacacccagt ttaacgcggc
    10681 gctgccgtgg gacagtatcg atatggactt tatgaacctg aaccagactg cacatggcgg
    10741 tcgcgagttc ggcttcattg gcgcgcgtat gcgtcagcaa catgccgtgg ttaccggtca
    10801 ctggcaggat aaacaagccc atgagcgtat cggctcctgg atgcgccagg cggtctctaa
    10861 acaggatacc cgtcatctga aagtctgccg ttttggcgat aacatgcgtg aagtagcggt
    10921 caccgatggt gataaagttg ccgcacagat caagttcggt ttctccgtca atacctgggc
    10981 ggttggcgat ctggtgcagg tggtgaactc catcagcgac ggcgatgtta acgcgctggt
    11041 cgatgagtac gaaagctgct acaccatgac gcctgccaca caaatccacg gcgaaaaacg
    11101 acagaacgtg ctggaagcgg cgcgtattga gctggggatg aagcgtttcc tggaacaagg
    11161 tggcttccac gcgttcacca ccacctttga agatttgcac ggtctgaaac agcttccagg
    11221 tctggccgta cagcgtctga tgcagcaggg ttacggcttt gcgggcgaag gcgactggaa
    11281 aaccgccgcc ctgcttcgca tcatgaaggt gatgtcaacc ggtctgcagg gcggcacctc
    11341 ctttatggag gactacacct atcacttcga gaaaggtaat gacctggtgc tcggctccca
    11401 tatgctggaa gtctgcccgt cgattgccgt agaagagaaa ccgatcctcg acgttcagca
    11461 tctcggtatt ggtggtaagg acgatcctgc ccgactgatc ttcaataccc aaaccggtcc
    11521 agcgattgtc gccagcctga ttgatctcgg cgatcgttac cgtctgctgg ttaactgtat
    11581 cgacacggtg aaaacaccgc actccctgcc gaaactgccg gtggcgaatg cgctgtggaa
    11641 agcgcaaccg gatctgccaa ctgcttccga agcgtggatc ctcgctggtg gcgcgcacca
    11701 taccgtcttc agccatgcgc tgaacctcaa cgatatgcgc cagttcgccg agatgcacga
    11761 cattgaaatc acggtgattg ataacgacac ccgcctgcca gcgtttaaag acgcactgcg
    11821 ctggaacgaa gtgtattacg gatttcgtcg ctaagtagcc acatcaggta tgtaacgcct
    11881 gatgcgacgc tgacgcgtct tatctggcct acacgctgcg attttgtagg ccggataagc
    11941 aaagcgcatc cggcattgtt ttccccctcg cccctttggg gagagggccg gggtgagggg
    12001 aaacgacctc tcaggagtca acatgttaga agatctcaaa cgccaggtat tagaagccaa
    12061 cctggcgctg ccaaaacaca acctggtcac gctcacatgg ggcaacgtca gcgccgttga
    12121 tcgcgagcgc ggcgtctttg tgatcaaacc ttccggcgtc gattacagcg tcatgaccgc
    12181 tgacgatatg gtcgtggtta gcatcgaaac cggtgaagtg gttgaaggta cgaaaaagcc
    12241 ctcctccgac acgccaactc accggctgct ctatcaggca ttcccctcca ttggcggcat
    12301 tgtgcatacg cactcgcgcc acgccaccat ctgggcgcag tcgggtcagt cgattccagc
    12361 aaccggcacc acccacgccg actatttcta cggcaccatt ccctgcaccc gcaaaatgac
    12421 cgacgcagaa atcaacggcg aatatgagtg ggaaaccggt aacgtcatcg tagaaacctt
    12481 tgaaaaacag ggtatcgatg cagcgcaaat gcccggcgtt ctggtccatt cccacggccc
    12541 gtttgcatgg ggcaaaaatg ccgaagatgc ggtgcataac gccatcgtgc tggaagaggt
    12601 cgcttatatg gggatattct gccgtcagtt agcgccgcag ttaccggata tgcagcaaac
    12661 gctgctggat aaacactatc tgcgtaagca tggcgcgaag gcatattacg ggcagtaatg
    12721 actgtataaa accacagcca atcaaacgaa accaggctat actcaagcct ggttttttga
    12781 tggattttca gcgtggcgca ggcaggtttt atcttaaccc gacactggcg ggacaccccg
    12841 caagggacag aagtctcctt ctggctggcg acggacaacg ggccgttgca ggttacgctt
    12901 gcaccgcaag agtccgtggc gtttattccc gccgatcagg ttccccgcgc tcagcatatt
    12961 ttgcagggtg aacaaggctt tcgcctgaca ccgctggcgt taaaggattt tcaccgccag
    13021 ccggtgtatg gcctttactg tcgcgcccat cgccaattga tgaattacga aaagcgcctg
    13081 cgtgaaggtg gcgttaccgt ctacgaggcc gatgtgcgcc cgccagaacg ctatctgatg
    13141 gagcggttta tcacctcacc ggtgtgggtc gagggtgata tgcacaatgg cactatcgtt
    13201 aatgcccgtc tgaaaccgca tcccgactat cgtccgccgc tcaagtgggt ttctatagat
    13261 attgaaacca cccgccacgg tgagctgtac tgcatcggcc tggaaggctg cgggcagcgc
    13321 atcgtttata tgctggggcc ggagaatggc gacgcctcct cgcttgattt cgaactggaa
    13381 tacgtcgcca gccgcccgca gttgctggaa aaactcaacg cctggtttgc caactacgat
    13441 cctgatgtga tcatcggttg gaacgtggtg cagttcgatc tgcgaatgct gcaaaaacat
    13501 gccgagcgtt accgtcttcc gctgcgtctt gggcgcgata atagcgagct ggagtggcgc
    13561 gagcacggct ttaaaaacgg cgtctttttt gcccaggcta aaggtcggct aattatcgac
    13621 ggtatcgagg cgctgaaatc cgcgttctgg aatttctctt cattctcgct ggaaactgtc
    13681 gctcaggagc tattaggcga aggaaaatct atcgataacc cgtgggatcg aatggacgaa
    13741 attgaccgcc gtttcgccga agataaacct gcgctggcaa cttataacct gaaagattgc
    13801 gagctggtga cgcagatctt ccacaaaact gaaatcatgc catttttact cgaacgagca
    13861 acggtgaacg gcctgccggt ggatcggcac ggcggttcgg tggcagcgtt tggtcatctc
    13921 tattttccgc gaatgcatcg cgctggttat gtcgcgccta atctcggcga agtgccgccg
    13981 cacaccagcc ctggcggcta cgtgatggat tcacggccag ggctttatga ttcagtgctg
    14041 gtgctggact ataaaagcct gtacccgtcg atcatccgca cctttctgat tgatcccgtc
    14101 gggctggttg aaggcatggc gcagcctgat ccagagcaca gtaccgaagg ttttctcgat
    14161 gcctggttct cacgggagaa acattgcctg ccggagattg tgactaacat ctggcacggg
    14221 cgcgatgaag ccaaacgtca gggtaataaa ccactttcgc aggcgctgaa gatcattatg
    14281 aatgcctttt atggcgtgct tggcaccacc gcctgccgct ttttcgatcc gcgcctggca
    14341 tcgtcgatca ccatgcgtgg tcatcagatc atgcggcaaa ccaaagcatt gattgaagcg
    14401 cagggctacg acgttatcta cggagatacc gactcgactt ttgtctggct aaaaggtgca
    14461 cattcggaag aagaggcggc gaaaatcggt cgtgcactgg tgcagcacgt taacgcctgg
    14521 tgggcggaaa cgctgcaaaa acaacggctg accagcgcat tagaactgga gtatgaaact
    14581 catttctgcc gttttcttat gccaaccatt cgcggagctg ataccggcag taaaaagcgc
    14641 tacgccgggc tgattcagga gggcgataaa cagcggatgg tgtttaaagg gctggaaacc
    14701 gtgcgcaccg actggacgcc gctggcccag cagtttcagc aggagctata cctgcgcatc
    14761 ttccgcaacg agccatatca ggaatatgta cgcgaaacca tcgacaaact gatggcgggt
    14821 gaactggatg cgcgactggt ttaccgtaaa cgccttcgcc gtccgctgag cgagtatcag
    14881 cgtaatgtgc cgcctcatgt acgcgccgct cgccttgccg atgaagaaaa ccaaaagcgt
    14941 ggtcgcccct tgcaatatca gaatcgcggc accattaagt acgtatggac caccaacggc
    15001 ccggagccgc tggactacca acgttcacca ctggattacg aacactatct gacccgccag
    15061 ctacaacccg tggcggaggg aatactccct tttattgagg ataattttgc tacacttatg
    15121 accgggcaac ttgggctatt ttgagcaaaa aaagagttcg ccagatacca ttttgatgcg
    15181 tgacgaatgc tttgccatcc agtaccatag cgccctttcc attcctggac ctgaataaca
    15241 ccactacccc ataagcacgg tagcgggtgg ttattgcctg caattaaaga tatagagccg
    15301 aacacatatg ccttttacac ttggtcaacg ctggatcagc gatacagaaa gcgaattggg
    15361 acttggaacc gttgtcgcgg tggatgcgcg aactgtcact ttacttttcc catctactgg
    15421 tgaaaaccgt ctgtacgcac gcagtgattc ccccgtgacc cgcgtgatgt tcaaccctgg
    15481 tgatactatt accagccatg acggctggca gatgcaagtc gaagaagtaa aagaagaaaa
    15541 tggcttgctg acctatatcg gtactcgcct ggatactgaa gagtccggcg tagccctgcg
    15601 tgaagttttc cttgatagca aactggtgtt cagcaaaccg caggaccgtc tgtttgccgg
    15661 gcagattgac cgtatggacc gctttgcgct gcgttatcgc gcgcgtaagt attccagcga
    15721 acagttccgt atgccgtaca gcggcctgcg cggtcagcgt accagcctga tcccgcatca
    15781 gctcaacatc gctcatgatg ttggtcgccg ccacgcgccg cgcgtcctgc tggctgacga
    15841 agtgggttta gggaaaacca ttgaagccgg gatgatcctg catcagcaac tgctctctgg
    15901 cgctgctgaa cgtgtgctaa ttatcgtccc ggaaacctta cagcatcagt ggctggtaga
    15961 aatgctgcgc cgtttcaacc tgcgctttgc gctatttgat gatgagcgtt atgccgaagc
    16021 tcagcacgat gcttacaacc cgttcgacac cgaacagctg gtgatttgct cgctggattt
    16081 tgcccgtcgt agcaaacagc gcctggaaca tctctgtgaa gccgaatggg acctgctggt
    16141 ggtcgatgaa gcgcatcacc tggtgtggag cgaagatgcg ccaagccgtg aatatcaggc
    16201 cattgaacaa ctggcagagc acgtgccggg cgttctgctg ctgaccgcga ccccggaaca
    16261 gctggggatg gaaagccact tcgcccgtct gcgtctgctg gacccgaacc gtttccacga
    16321 ttttgcgcag ttcgttgaag agcagaaaaa ttatcgtccg gttgcggacg ccgttgccat
    16381 gctgctggca ggtaacaaac tgagcaatga cgaactgaac atgctcggcg agatgatcgg
    16441 cgagcaggat atcgagccgc tgttgcaggc agcaaacagc gacagcgaag atgcccagag
    16501 cgcccgtcag gagctggttt cgatgctgat ggatcgccac ggcaccagcc gcgtgctgtt
    16561 ccgtaacacg cgtaacggtg tgaaaggatt cccgaaacgc gagctgcaca ccattaagct
    16621 gccgctgccg acgcagtatc agacggctat taaagtctcc ggcattatgg gcgcacgtaa
    16681 aagtgcggaa gatcgtgctc gcgatatgct ctacccggag cgtatttatc aggaatttga
    16741 aggtgataac gccacctggt ggaacttcga tccgcgcgtt gagtggctga tgggctacct
    16801 gaccagccat cgctctcaga aagtgctggt gatctgcgcc aaagctgcca ctgcgctgca
    16861 actggagcag gtactgcgcg aacgtgaagg tattcgcgct gcggtgttcc acgaaggtat
    16921 gtcgattatc gaacgtgacc gcgctgccgc ctggtttgcc gaagaagaca ccggcgcaca
    16981 ggtactgctg tgctcagaaa tcggttctga aggacgtaac ttccagttcg ccagccacat
    17041 ggtgatgttt gacctgccat tcaacccgga tctactggag cagcgtattg gtcgtctgga
    17101 tcgtatcggc caggcgcacg atattcagat ccatgtgcct tatctggaga aaaccgctca
    17161 gtcggtgctg gtgcgctggt atcacgaagg tctggatgca tttgagcaca cctgcccgac
    17221 cggacgcact atttacgata gcgtatacaa cgatctgatt aactatctgg cttcaccggt
    17281 tcaaaccgaa ggctttgacg atctgatcaa aaactgccgc gagcaacatg aagcgctgaa
    17341 agcacagctg gaacagggtc gtgaccgcct gctggaaatc cactccaacg gtggcgaaaa
    17401 agcccaggca ctggcagaaa gcattgaaga gcaggatgac gataccaacc tgatcgcctt
    17461 cgccatgaac ctgttcgata ttatcggtat caatcaggac gatcgcggcg acaacatgat
    17521 cgtgctgacg ccgtccgatc atatgctggt gccggacttc cctggcctgt cggaagatgg
    17581 catcaccatc acctttgatc gtgaagtggc gctggcgcgt gaagatgcac agtttattac
    17641 ctgggagcat ccgctgatcc gcaacggtct ggatctgatc ctttctggcg atactggtag
    17701 cagcacgatt tcactattga aaaacaaagc gttgccggta ggtacgctgt tggtggaact
    17761 gatttatgtc gttgaagccc aggctccgaa gcagttgcag ctcaaccgct tcctgccacc
    17821 gacgccggta cgtatgctgc tggataaaaa cggcaacaac ctggcggcgc aggtagagtt
    17881 tgaaaccttt aaccgtcagc ttaacgcggt taaccgtcac accggcagca aactggttaa
    17941 cgccgtgcag caggatgttc acgctatcct tcagctgggt gaagcgcaga tcgagaaatc
    18001 tgcccgtgca ttgattgatg ccgcacgtaa cgaagccgac gaaaaactgt ctgccgagct
    18061 gtctcgtctg gaagcgctgc gcgcagtgaa cccgaacatt cgtgacgacg aactgaccgc
    18121 cattgagagc aaccgtcagc aggtaatgga aagcctggat caggcaggct ggcgtctgga
    18181 tgccctgcgt ttgatcgttg taacgcatca gtaacggagc cgaaaatggg gatggaaaac
    18241 tacaatccac cgcaggaacc ctggttggtt atcctgtatc aggatgacca cattatggtg
    18301 gttaacaagc cgagcggttt gttgtctgtg ccggggcgtc tggaagagca caaagacagc
    18361 gtgatgacgc gcattcagcg tgattatccg caggcagaat cggtgcatcg tctggatatg
    18421 gctaccagcg gcgtgattgt agtggcgctg accaaagccg cggagcggga gttaaaacgc
    18481 cagttccgcg agcgcgagcc gaaaaagcag tatgtagccc gcgtctggga gcatccatcc
    18541 cctgcggaag gtctggtgga tctgccgctg atttgcgact ggccaaaccg cccgaaacag
    18601 aaagtctgtt acgaaacggg taaacctgcg cagacggaat atgaagtggt ggagtatgcg
    18661 gcggataaca cggcaagagt ggtgttaaaa ccgattaccg ggcgttcgca tcagctgcgt
    18721 gtgcatatgc tggcgctggg tcatccgatt ctcggcgatc gtttttatgc atcaccagaa
    18781 gcgagagcga tggcaccgcg tttgttgcta catgcagaga tgttgacgat tacccatccg
    18841 gcgtatggca atagtatgac gtttaaagcg ccagcggatt tttaatcgtc acgctggcgc
    18901 agttcggtag gcggataagg cgttcacgcc gcatccggca attgtgcgcc gattgcctga
    18961 tgcgacgcgg gccgcgtctt atcaggccta cagtttaaaa cttatttaaa ccctttctgc
    19021 tgctttatca gctcatatgc ctgctgaatt tcctgcgctt tctgcttcgc catctccatc
    19081 atctcaggcg gcaaaccttt cgccaccagc ttatcgggat ggtgttcact catcagctta
    19141 cggtaggcac gtttgatggt ggtcgcatca tccgtcggct tcacgcccag cacattacag
    19201 gcatcttcca gcgtcgggcc acgctgcgct tgctgccagt taccaccgcc agattgctgc
    19261 tgataaccgc cgccaaactg tgcaccgccc tgcatcatgc gcaaaaactg gtcaaactgg
    19321 gcgcgggaga tccctaattc ttcagcaatg acatacagca ccgcccgttc attcgggtgc
    19381 agtgaaccat cagcaaacgc cgcctgaatc tggatctcca gaaacatacg aattaagtca
    19441 aaacgaccaa agcagacact gcgaaactgg cgcatctttt cgcgcagcgg gtaattatct
    19501 gattttccca cccggaacgc gttttgcgcc gcagtacggg aagcgccatg aaggttcatt
    19561 cggtccatca actggctggc gatatgaata tcagcctccg tgacgcgacc tttggatttg
    19621 gttaaatgcc ccatcacttc aaaagtggtg gcaaaaaaca gcgcctgacg ctcacgctgg
    19681 ttggcgaacc acgccatttt acggctacgg gctttatcaa acatatggcc aattaacagg
    19741 cctaacacta cgccccaaaa gccgccgccc atcagtaagg ccacggccac gccaatgatt
    19801 tttccccaat actgcatata ttccccaaat cgacacacgg atatcagggc tatctcccac
    19861 aatataaagg tgcttttacc gttttccggc tgcggtcaat tgtgggacat cgcctataat
    19921 ttgcattatc atacctgtca ttcaatgccg tgcctaacac cactgacgct attcggacag
    19981 gattaacact agcgtagaga tgacgagtac gttagtctct gaccgtttgt cacgcgcaac
    20041 gttaccgatg atggaacaat aaaatcaacg tatgaaaaaa cgtatcccca ctctcctggc
    20101 caccatgatt gccaccgccc tttatagtca acagggactg gcagccgacc tcgcctcaca
    20161 gtgcatgttg ggcgtgccaa gctatgaccg tcctctggta cagggcgata ccaatgactt
    20221 acccgtgact atcaatgctg accacgcgaa aggggactac ccggatgacg ccgtgtttac
    20281 tggcagcgtg gatatcatgc agggtaacag ccgtctgcag gccgacgaag tgcagctcca
    20341 tcaaaaagag gcaccaggac aaccggagcc ggtacgtacc gttgatgcgc tcggtaatgt
    20401 ccattacgac gataaccagg tgatcctcaa agggccgaaa ggctgggcga atctgaacac
    20461 caaagatacc aacgtctggg aaggtgatta ccagatggtg ggtcgccagg gtcgcggtaa
    20521 agcggacctg atgaaacaac gtggcgaaaa ccgctatacc attctggata acggtagctt
    20581 tacctcctgt ctgccgggtt ctgacacctg gagcgtggta ggtagcgaaa ttattcatga
    20641 ccgcgaagaa caagttgcgg agatctggaa cgcccgcttt aaggtgggtc cggtaccgat
    20701 cttttatagc ccctatttgc agttgccggt gggtgacaaa cgtcgctctg gtttcttgat
    20761 cccgaacgcc aagtacacca ccaccaacta ctttgagttc tacctgccat attactggaa
    20821 catcgcgcca aatatggatg ccaccatcac gccgcattat atgcatcgtc gtggcaacat
    20881 catgtgggag aacgaattcc gctacctctc ccaggcgggc gctggcttga tggaactgga
    20941 ctatctgcct tcagataaag tctatgaaga tgaacacccg aacgatgaca gttcacgtcg
    21001 ttggttgttc tactggcaac actccggggt catggatcaa gtgtggcgtt tcaacgtcga
    21061 ctacaccaag gtcagcgatc ctagctactt caatgatttc gataacaagt acggttccag
    21121 tactgacggc tacgcaacgc aaaaattcag cgttggctat gcggtgcaaa acttcaatgc
    21181 caccgtttca accaagcagt tccaggtctt tagcgagcag aacaccagta gctactcggc
    21241 agagccgcag ttagacgtta actactacca gaatgatgtt ggtccgtttg atacgcgtat
    21301 ttacggccag gcagtgcact ttgttaacac cagagacgac atgcctgaag caacccgtgt
    21361 tcacctggaa ccgaccatca atttgccgct ctctaataac tggggcagca tcaataccga
    21421 agcgaagttg ctggcaaccc attatcagca aaccaatctt gactggtata actccagaaa
    21481 cacgaccaag ctggacgaat ccgttaaccg cgttatgccg caattcaaag ttgacggcaa
    21541 aatggtcttt gaacgcgata tggaaatgct ggctccgggt tatacccaaa cgctggaacc
    21601 gcgcgcgcag tatttgtacg tgccgtatcg cgatcagagc gacatctata actacgactc
    21661 gtctctactg caatctgact actctggcct gttccgggac cggacttacg gcggtcttga
    21721 ccgtattgcc tccgctaacc aggtgacgac cggtgtcaca tctcgcatat atgatgatgc
    21781 tgccgttgaa cgttttaata tttccgttgg tcaaatctac tatttcacgg agtctcgcac
    21841 tggcgatgac aacataacat gggagaatga cgacaaaacg ggctcactgg tgtgggcagg
    21901 cgatacttac tggcgtatct ccgagcgttg gggattgcgt ggcgggattc agtacgatac
    21961 acgtctggat aacgtagcga ccagtaactc cagcattgaa taccgtcggg atgaagaccg
    22021 tctggtacag ctgaattacc gttacgccag cccggaatat attcaggcta cgctgcctaa
    22081 gtactattcc actgctgagc aatataagaa tggtatttcg caggtaggtg ctgtcgccag
    22141 ctggccaatt gccgatcgtt ggtccattgt tggggcctac tactacgaca ccaatgcgaa
    22201 caagcaagcc gactctatgt taggtgtgca atacagctcc tgctgctatg caattcgcgt
    22261 cggttacgag cggaagctga acggttggga taacgataaa caacatgcgg tatatgacaa
    22321 cgcaatcggc tttaacatcg aacttcgcgg cctgagctcc aactacggtc tgggtacgca
    22381 agagatgctg cgttcgaaca ttctgccgta tcaaaacact ttgtgatttg ttgatttacc
    22441 acgtaatccg cagtgcggtt aattgaaatg gaaaaagtat gaagaactgg aaaacgctgc
    22501 ttctcggtat cgccatgatc gcgaatacca gtttcgctgc cccccaggta gtcgataaag
    22561 tcgcagccgt cgtcaataac ggcgtcgtgc tggaaagcga cgttgatgga ttaatgcagt
    22621 cggtaaaact gaacgctgcg caggcaaggc agcagcttcc tgatgacgcg acgctgcgcc
    22681 accaaatcat ggaacgtttg atcatggatc aaatcatcct gcagatgggg cagaaaatgg
    22741 gagtgaaaat ctccgatgag cagctggatc aggcgattgc taacatcgcg aaacagaaca
    22801 acatgacgct ggatcagatg cgcagccgtc tggcttacga tggtctgaac tacaacacct
    22861 atcgtaacca gatccgcaaa gagatgatta tctctgaagt gcgtaacaac gaggtgcgtc
    22921 gtcgcatcac catcctgccg caggaagtcg aatccctggc gcagcaggtg ggtaaccaaa
    22981 acgacgccag cactgagctg aacctgagcc acatcctgat cccgctgccg gaaaacccga
    23041 cttctgatca ggtgaacgaa gcggaaagcc aggcgcgcgc cattgtcgat caggcgcgta
    23101 acggcgctga tttcggtaag ctggcgattg ctcattctgc cgaccagcag gcgctgaacg
    23161 gcggccagat gggctggggc cgtattcagg agttgccggg tatcttcgcc caggcattaa
    23221 gcaccgcgaa gaaaggcgac attgttggcc cgattcgttc cggcgttggc ttccatattc
    23281 tgaaagttaa cgacctgcgc ggcgaaagca aaaatatctc ggtgaccgaa gttcatgctc
    23341 gccatattct gctgaaaccg tcgccgatca tgactgacga acaggcccgt gtgaaactgg
    23401 aacagattgc tgccgatatc aagagtggta aaacgacttt tgctgccgca gcgaaagagt
    23461 tctctcagga tccaggctct gctaaccagg gcggtgatct cggctgggct acaccagata
    23521 ttttcgatcc ggccttccgt gacgccctga cccgcctgaa caaaggtcaa atgagtgcac
    23581 cggttcactc ttcattcggc tggcatttaa tcgaactgct ggatacccgt aatgtcgata
    23641 aaaccgacgc tgcgcagaag gatcgtgcat accgcatgct gatgaaccgt aagttctcgg
    23701 aagaagcagc aagctggatg caggaacaac gcgccagcgc ctacgttaaa atcctgagca
    23761 actaatggtt aaaacccaac gtgttgtgat cactcccggc gagcccgccg ggattggccc
    23821 ggacttagtt gtccagcttg cacagcgtga gtggccggtc gaactggttg tttgtgccga
    23881 tgccactctc cttaccgacc gggcagcgat gctcggtttg ccgctcaccc tccgccctta
    23941 ttcccccaac tcccctgcac aaccgcaaac tgcgggcaca ttaacgctac ttcctgtcgc
    24001 gctacgcgaa tctgtcactg cggggcagtt agcgattgaa aatgggcatt acgtggtgga
    24061 gacgctggcg cgagcgtgtg atggctgtct gaacggtgaa tttgctgcgc tgatcacagg
    24121 ccccgtgcat aaaggcgtca ttaacgacgc aggtattccg tttactggtc ataccgagtt
    24181 tttcgaagag cgctcgcagg cgaaaaaagt ggtgatgatg ctggcgaccg aagaacttcg
    24241 cgtggcgctg gcaacgacgc atttaccgct gcgcgatatc gcagatgcta tcacccctgc
    24301 acttttgcac gaagtgattg ctattttgca tcacgatttg cgtacaaaat ttggtattgc
    24361 cgaaccgcgc attctggtct gcgggctgaa tccgcacgcg ggcgaaggcg gtcatatggg
    24421 tacggaagag atagacacca ttattccggt gctcgacgag ctgcgggcgc aggggatgaa
    24481 actcaacggg ccgctgcctg ccgataccct gtttcagccg aaatatctcg ataacgccga
    24541 cgccgtgctg gcgatgtacc acgatcaggg tcttcccgtg ctaaaatacc agggcttcgg
    24601 gcgcggtgtg aacattacgc tgggcctgcc ctttattcgc acatcagtgg accacggcac
    24661 cgcgcttgaa ctggcgggac gtggcgaagc cgatgtcggc agttttatta cggcgcttaa
    24721 tctcgccatc aaaatgattg ttaacaccca atgaataatc gagtccacca gggccactta
    24781 gcccgtaaac gcttcgggca aaactttctc aacgatcagt tcgtgatcga cagcattgtt
    24841 tctgccatta acccgcaaaa gggccaggca atggtcgaaa tcggccccgg tctggcggca
    24901 ttgaccgagc cggtcggcga acgtctggac cagctgacgg tcatcgaact tgaccgcgat
    24961 ctggcggcac gtctgcaaac gcatccattc ttaggcccga aactgacgat ttatcagcag
    25021 gatgcgatga cctttaactt tggcgaactg gccgagaaaa tgggtcagcc gctgcgtgtt
    25081 ttcggcaacc tgccttataa catctccacg ccgttgatgt tccatctgtt tagctatact
    25141 gatgccattg ccgacatgca ctttatgttg caaaaagagg tggtgaatcg tctggttgca
    25201 ggaccgaaca gcaaagcgta tggtcgatta agcgtcatgg cgcaatacta ttgcaacgtg
    25261 atcccagtgc tggaagtacc gccgtcagcc tttacaccac cacccaaagt ggattccgcc
    25321 gtcgtgcgcc tggttcctca tgcaacgatg cctcacccgg ttaaagatgt tcgcgtgttg
    25381 agccgcatca ccactgaagc ctttaaccag cgtcgtaaaa ccattcgtaa cagcctcggc
    25441 aacctgttta gcgtcgaggt gttaacggga atggggatcg acccggcgat gcgagcggaa
    25501 aatatctctg tcgcgcaata ttgccagatg gcgaactatc tggcggagaa cgcgcctttg
    25561 caggagagtt aacgatgatc aattcgcccc gagtgtgtat tcaggttcaa agcgtctaca
    25621 ttgaggcaca atcttcacct gataatgaac gttacgtttt tgcttatacc gtaaccatac
    25681 gcaatctggg gcgagcgcca gtgcagttgt tggggcgtta ctggctgatc accaatggca
    25741 atggccgtga aaccgaagtc cagggcgaag gagtggttgg cgtccagcca cttatcgcgc
    25801 ctggcgaaga gtaccagtac accagcggtg caatcattga aaccccgctg ggcaccatgc
    25861 agggtcacta cgaaatgatc gatgaaaatg gcgtcccttt cagcatcgac attcccgtat
    25921 tccgactcgc cgttcccaca ctcattcatt aaaagaatat ggcgacatac cttattggcg
    25981 acgttcatgg ttgttacgat gaactgatcg cattgctgca taaagtagaa tttacccctg
    26041 ggaaagatac cctctggctg acgggcgatc tggtcgcgcg cgggccgggt tcgctggatg
    26101 ttctgcgcta tgtgaaatcc ttaggcgaca gcgtacgtct ggtgctgggc aatcacgatc
    26161 tgcatctgct ggcggtattt gccgggatca gccgcaataa accgaaagat cgcctgacac
    26221 cgctgctgga agcgccggat gccgacgagc tgcttaactg gctgcgtcgc cagcctttgc
    26281 tgcaaatcga cgaagagaaa aagttggtga tggcccacgc agggatcacg ccgcagtggg
    26341 atctacagac cgccaaagag tgcgcgcgcg atgtagaagc ggtgctatcg agtgactcct
    26401 atcccttctt tctcgacgct atgtacggcg atatgccaaa taactggtca ccggaattgc
    26461 gggggctggg aagactgcgt tttatcacca acgcttttac ccgtatgcgt ttttgcttcc
    26521 cgaacggtca actggatatg tacagcaaag aatcgccgga agaggcccct gccccactga
    26581 aaccgtggtt tgcgattcct ggccctgtcg ctgaagaata cagcatcgcc tttggtcact
    26641 gggcatcgct ggagggcaaa ggtacgccgg aaggtatata cgcgctggat accggctgct
    26701 gctggggtgg tacattaacc tgcctgcgct gggaagataa acagtatttt gtccagccgt
    26761 cgaaccggca taaggatttg ggcgaagcgg cggcgtctta aacacagcct gatataggaa
    26821 ggccggataa gacgcgaccg gcgtcgcatc cggcactagc cgtaaattct atacaaaatt
    26881 accgccgctc cagaatctca aagcaatagc tgtgagagtt ctgcgcatca gcatcgtgga
    26941 attcgctgaa taccgattcc cagtcatccg gctcgtaatc cgggaaatgg gtgtcgcctt
    27001 ccacttctgc gtcgatatgc gtcagataca gtttctgcgc ttttggcagg aactgctcat
    27061 aaacgcgacc gccgccaatc accatgattt ctggtacgtc accacacgcc gcgatggctt
    27121 catccaccga cttcacccac gttacgcgat cgtccgtacc cggttgactg ctgaggataa
    27181 tatttttgcg tcctggcaac ggacgaccga ttgattccca agtatggcgg cccataatca
    27241 cgggtttatt taaggtgttg cgtttaaacc aggcgagatc ggcaggcagg ttccacggca
    27301 tggcgttttc catgccgata acgcgatcta ccgctaacgc cgcaatcaga ctgatcattg
    27361 agatttcccg ataaaaaaaa ttgtcgccac tatacgtaaa gcgtaaaccg tcgtcgactg
    27421 gtgcgaggat gatgttgagg aaaattttat attctgctgg cgagtccacg ctctctccct
    27481 ggactcgccg cattacaatg aaacaaaaac aaacagttag ctgtaaagtg tgatttacgt
    27541 cactctttat taggatgagg gtttcgtttc cggttcatcc gccatgttgc cggtatgttt
    27601 accttcttcg gttccctgcc agccatgtcg ttgaattaat gacagatgtt cgcggtcctc
    27661 ggtaatgatc tcacttaaca tcgcgctggt gcgtttatag accgccgcgc gggctttggt
    27721 gtcgttctca accattgcca tctcttccac catctgaata ttaaagcggc ggaacacatc
    27781 ggcacgttct cgcgcttcat acggccccag acctaaactt tccagtgcca gacgcccggt
    27841 tttcagcgca ccttcgaagg tttcacgctc cggcttttca acgcctgcct gacgcaaacg
    27901 aatgtagtgg tcgacatcgc gggcgcgggc aataatctgc aaatgcggga agtgttcttt
    27961 caccatctct gtcagttgca ggttggtttg cggatcgtcg atggcgttaa tcagcacttc
    28021 cgctttcgcc gctcccgcag attccagtaa atccatccgc gtggcatcgc cataaaacac
    28081 tttcatacca aatttacgca aggtttcgat atggtccgga tcgtgatcga gcaccaccat
    28141 tttcaccccg ctggagagca gtaaacgtcc ggtgatctgc ccaaaacgac cgaatccggc
    28201 aatgattacc cgcggctgtt cttcgtcgat ctcatcagct tcacgcgcct cttcagtaga
    28261 agattgctca aggcgattga ggatcaccag cagaatcggc gttgccgcca tcgacagcgc
    28321 caccgccagg gtcagcgatt tcgcccactc cggttccagc acattcgcca tctgcgccgt
    28381 gccaaatacc acaaaggcaa actcactgcc ctgccctaac aacaccgcaa accaacgacg
    28441 ctgtttattt ggcacttgca acggtcgggc aatcagccac agcatggcga ttttgatgat
    28501 gaggaaaccg agcagcaaaa tgacaatgcg caatgggttt tcaagcagcg tgccaaagtc
    28561 tatcgacatg ccaacaccga tgaaaaacag ccccaacagc aaacctttaa atggttcgat
    28621 atcgctctcc agcgcatgac ggtattccga gcttgccagc agtacgcccg ccagaaacgc
    28681 gcccatcgcc atcgacaagc cgacctcttc cagcagcaaa ccaaagccaa acacgaggaa
    28741 taacgccacg gcactaaaca cttcccgcaa gccagagcgg gctacaaaac gcagcgccgg
    28801 acgcgtgaca tagcgcccca gcaataccac cagcaccagc gcacccgcca cttttaacgc
    28861 cgagagagca aatgcgccca tcgtcgtcga ggcactgctc gttgccagta gcggaatcat
    28921 cgccaccagc gggatcgccg cgatatcctg gaacagcagc accgcaaagg cactgcgacc
    28981 catttgcgtc accatcagat tgcgttcatt catcgcctgc atggcaatcg ccgtagagga
    29041 gagcgccagc gtcatgccga tcaattccgc gacctgccag cgcagcccaa gtaacatgca
    29101 gaacagcccc agcaggccgc cgcaaatcac catctgcaat gcgccaccgc cgaacactgc
    29161 cgcacgcagc ttccacagcc tttgtggatc gagttcgagg ccgataataa acagcatcag
    29221 caccacccca atctcggcaa agtgcagaat agattcggca tcggtcacca gtcgcagccc
    29281 ccacgggcca ataatgcagc cggcgatcag gtagccaagt accgatccca gaccaagacg
    29341 taccgcaatg ggtacaatca gcgctgccga accgagataa atcagcgcct gaatcagcgt
    29401 atggctatcc atgatgggcc tcctgccatt ccagcagacg ttgcttatag tgacgcgcct
    29461 gcccttcgag ggtttcgtcg tcacaaataa aggtgcagtg catggcaaac ggtggcagcc
    29521 agttcagccc gcagtagatt gccgtcgcct gtagcggctg cgacagcaca tcaaagcccg
    29581 gatgcgcacc aatttcaaaa tggctttccc cgccgccggt cgtcaccgcc cacagcaaat
    29641 gtttgccatg cagcgccttg ccgccgtgac cgtaagccca gccgtgcgac aaaactttat
    29701 cgatccaaag tttaagaagc ggaggaatgc tgtaccactg catcggatgc tgccagacga
    29761 tcagatcggc gcgagacagc gcctcctgct cggcggcaat atcgatattg aagtcaggat
    29821 agagttgata aagagagcga atttcgacgc cttccagcgt ccttgcctgt tcaagcatcc
    29881 gtttattcgc atgggaatga tgcggatacg gatgcgcata aattataaga atcataggta
    29941 gcccgcgtga ttacgccttt gttttcccac aaagtgtagt catatattca cgcggcttat
    30001 agtcaataat attgattcag aaaagagaga aatctgaaca atttacccca ttctctgccc
    30061 gcggatcgtc atattgctgg tttgcgccag tgacatccct cgagtctccg gggcaaacgc
    30121 tacggaaatc aacaagccaa acagcgagat acccgcctcc attagcatcg tgttactgat
    30181 accgtaatta ttgataaaga tcggcagtgc ccaggtcgaa acaatggtgc caatacggct
    30241 taaggacata atcacgccca cggcagaggc gcggatatct gtcgggaaga gttcattagg
    30301 atagagccac tgcaaattac ccggcccgcc agagaaaaag gcatacaccg caaaggccat
    30361 cactaccagc cagatcccca tatccgggat cagccccaaa accgccagcg ccagcgtcat
    30421 catggcaaag ctgccaatca acaatggacg ccgtccggca gtgtttaacc acagcatcgg
    30481 cggaatacag ccgagcataa agaacaggct aatcaccacg ttccccagcg ccgcgttttt
    30541 gccaacaccc aatcccaaca aaccaacgat ttgcgggcca aaggtgtaaa tggcgaacat
    30601 tgggatcacc tggcaggtcc agatggcggc aacaaacaga acaaaaggaa aatggcggcg
    30661 attaaacaga tcgcgaaaac gggtttgcta cggctgctct tcatcgaaag ccaccggttc
    30721 gccaaacagt tttatcatca tctcttcgca ctcttttact cgccctttgc gtaataacca
    30781 gcggggagat tcaggcagtt cgaatcgacc aatcaaaatc aacaaacagg ggatcgccgc
    30841 gctacccagc atccagcgcc agccgccttc cacatcataa agccagtagc cgaccagatc
    30901 ggcgcaggtc gcgccgacat accacatggc ggcgataaag ctgatggaaa atgcccgctg
    30961 acgggtactg gagaactcgg tgatcattga ggtggcgatg ggataatctg caccgatgac
    31021 aatgccgata agtacccgca tcaccaacag ttcgacgggg gatgaaacaa acatcgtcgc
    31081 caccgatatc acgccgatgg cgatgatatc aatgaggaac attttgcgcc gtccgacttt
    31141 atcggaaata tagccaaaca gcgatgtgcc aacgaacagc ccggcgagcg ttcccgcgcc
    31201 caacaagcca atccagtcag cgtccagttt tagcgccgga gtcagttgct ccagcgccac
    31261 gccaatcatt accagtacat aaccatccag aaacggacca ccgcttcccc acagcaaaat
    31321 gcggcggtga attgaggaga atttgagatc gtcaaagttt ctggacggtt gcatgggtta
    31381 ttcctgtttt tttgcaatga gagtcagtac ggcgttacgt atcaaaccaa catcagccgt
    31441 aacggaactc cacaccaaag gtgccgcgcg ggtattccca ctgttccagc gccgacccca
    31501 gccccagaat gcgataggtg ccgcactcca gacatccggc gtaatcgaag cgcacactgc
    31561 cgtcatcctg cttcttgtac agacctgcgg ggcacgcttt caccagcagc tccagggcct
    31621 gtttatcggc atcggccttc acaacaatgt gcggatgctc ttcatcgaca ttgaatttat
    31681 tgacgcccag tttgacgtcc acattgacgg gagaagtcat aaaacggtca ctcctttcat
    31741 gccatccttg atcagattga tgaagcccac tttcttgccg tggcggagga ttttcttgcg
    31801 catcagttcc ggcgcactgc cgtcaatggt gaacaggtca cgcgccacgc ccaccgccag
    31861 ttccgggtag ccgctaaaca tgcgtgggtt atcaaggaag gccggtagtt tctggtacat
    31921 acgcatatcg cgcagcgggc cactctcaag atgctgacga tattccgcca gtttttgctt
    31981 actgaaatcg tcgcttttca tcgctgaaag cacggttttt gctgcggctt ccccggcggc
    32041 aatcgccaga tccataccgc gaatggtaaa accgaggttc atacacattc cggcggcatc
    32101 accggcaatc aatacgccgt caccaaccaa ctccggcagc atgttgatgc ctgcttccgg
    32161 cactacgtga gcggaatatt ccaccagctt gccgcccgcg atcagcggtg ccactgccgg
    32221 atgctgtttg aaatcttcca gcatttgcgg caccgatttt tttgcgtcat gcagatgatg
    32281 cagaccacaa accagcccca gcgacagggt attttcattg gtataaagga agccgccgcc
    32341 catcaggcca tcggtgggtg atcccgcaaa cagacaagcc gcgccctgat taccctgcaa
    32401 ctgaaaacgg tcttcgatta ccgacttcgg taactcgatc agttccttca cgccaaccgc
    32461 cacatccgtc ggtttaacgc gttttgccat ccccagcttt tcggcaagga tggagttcac
    32521 tccatcagca aggatcaccg ttttcgcttc aatcacatcg ccatcggctt ctacaccgac
    32581 gactttgcca tcgcgctgta cgaggttatc gacgcggatc ccggtaatta actgcgcgcc
    32641 cgcttcttcg gcctgctcca tcagccaggc atcaaattta ctgcgcaaaa cggagtaaga
    32701 acgctgggat ggcgaggttt cgtcaccgtt gcagtagtcc atagtcatcg ctgacttttc
    32761 cgtcataaac gcgagttttt catgggtgat caggcgttct accggggcgg attcggcgaa
    32821 accaggaata atgtgttcca ggctgtgggc atagagacgc ccgccggtga cgttcttggc
    32881 acctgcggaa ttgccacgct cgataactaa cacttgcgcc ccttcgcggg cgagcaccag
    32941 tgcggcaacc gaaccggcaa gcccagcacc gacgatgatg gcgtcaaaga tatcttcgga
    33001 cataagagct ccctgtcagc ggcagggcca aaatgcagcc ctgccagagt ggatcaacgc
    33061 gctaaagctg cggtcagcgc cggaaggatc ttcacggcgt caccaacaat gccgtaatcc
    33121 gcgtactgga agatcggcgc atttttatct ttattgatgg cgaaaatggt ttgcgatgca
    33181 ttagcaccaa ccatgtgctg gatctgcccg gagatcccca ccgccaggta cagttcaggt
    33241 ttcagcatca ggttggagat accgacatag cgttcgtgct ccatccattt ttcgttttcc
    33301 gccaccggac gagaacaggc caactccgca cctatcgcct tgcaaagctg ttctgccagc
    33361 gcaatgttct ctttgctgcc aataccgcga ccgacgctga ccaccagacg ggctttgtcg
    33421 agatcgacgc tgttgctctg gcgcgcctgg gttgccgtgc gggtaatcgc cacagccgga
    33481 gcctgccact ccacggtgtg cgtttcgcca gtgcgtgacg cgtctggctg agccgcatcg
    33541 aacgtgccgc tgctgatggt cagtaccgca tacggcgtgg caatgcgttc ttcgccaatc
    33601 gccagaccac cgtaaaccat atgtttcact gttgctttac cgtcctgtac gctgacggtg
    33661 ctggcatcgt tagacaccgc cgctttaagg cgataaccca gttttgccgc cagtaatttg
    33721 ccgcgacggg tgtttggcag cagcaccagg ccgtctgcgc cgtgctggcg aatagtgtca
    33781 gccatgacac cggcgtaatc ttcgatcatc cgatcgtccg gtttgccgtt taatttccag
    33841 acatgattag cgccgagctg gattgcctgt gcgccgtcgg catcattgag gacaaaggtg
    33901 ttgatttgat tagctaaagc ctgcgcaccg ttcatcagtt ccggcagacg agaaggggta
    33961 tcgctgaata cccagacttg agaaaacgtg ttcatagcat cccctgtaat taaatgactt
    34021 tgcgaagatt ttcagcaaat gcggcgatct gttcttcgcc gtcgccttca atcacgatgc
    34081 gctgacgttc gcgctgtttc ggcgcggcaa cctgttgttc tgaccaggct gcctctgcgt
    34141 taaaaccaat atccgccgcc gaccatacct ggacgggctt tttcgccgcg ccgagaatgg
    34201 ctttcatcga aggaatttgt ggggaattga tatcagtgga aacagcaaca accgcaggca
    34261 gcggaatgct taaggtttcg gtttcatctt ccagttcgcg ctcaacggtg agggtatctg
    34321 ccgtcaggga gataattttg ctgacgccgt taactgccgg aatattgagg atttcgccca
    34381 ccagcagacc aacctgctgg gcataaaggt cggaagaacc atcgccacag aggatcaaat
    34441 caaagcctgc tttctgggcg gctgcagcca gtgcgctcgc cgtttgttgc ggcagtgcct
    34501 gctcgaactg gtcatcaatc accacaatca gttcatccgg gccgcgcgat agtacatctt
    34561 tacgcccttt ggcgttggtc agggctttac cgcccacact taaggccgtc acctgcgcct
    34621 ctgctgcctg ttgctttagc tggcaagccg cttcaatagc gttcagatcg tattggctta
    34681 ttttggcatc ggctttgctg aagtctaatg aaccatcagc attattgacc gcaatatcct
    34741 gttcatcagg cacgcactta tagcaagtaa taatcttcat tgcatctcca gaaatcatga
    34801 aggaaacgtc gttcatatcg gtggtgttaa ttacagatat cacgcccttt aatttggaaa
    34861 taaaacttta atcaccaata ttgaaaatgt cacacgcaga taaatccaac tttcaatatt
    34921 gttaagttcc tcaccaatat tgaaaacatg gcgtagaaaa aagaaatttt caatattgct
    34981 ttatggatca ccaatattga aagtcagatg ctattttttt actcacaaca caacataaca
    35041 aactgattaa taatcaaaat taacgagaaa agcgccctct attcacccga aaaaaaatgt
    35101 gataacaatc acagaatgca gcttattgaa tacccattat gagttagcca ttaacgcgtc
    35161 cacgaggtta ataataatta tatcaaatgt taacaaaaat aaaacaaacg ggaacgcaaa
    35221 ataaatattc gttttcacag tggaattaac tcatgaagaa tgaaaagaga aaaacgggaa
    35281 tagaaccgaa ggttttcttt ccgccgttaa taatcgtcgg catactttgt tggcttacag
    35341 tcagagatct ggatgcagcg aatgtcgtta ttaatgctgt attcagttac gtcaccaatg
    35401 tatggggatg ggcatttgaa tggtatatgg tggtgatgct tttcggttgg ttctggctgg
    35461 tgtttggccc gtatgccaaa aagcgtttag gtaacgaacc acctgaattt agtaccgcca
    35521 gttggatctt tatgatgttc gcctcctgta cgtctgctgc cgtactgttc tggggatcga
    35581 ttgagatcta ctactacatc tccaccccgc cgtttggctt agaaccgaac tcgacagggg
    35641 cgaaagagtt ggggctggct tacagcttgt tccactgggg acctctgccg tgggccactt
    35701 acagcttcct ttcagtcgcc ttcgcttact tcttctttgt ccgcaaaatg gaagtgattc
    35761 gccccagctc gacactggtg ccgctggtag gtgaaaaaca cgccaaaggg ttgttcggca
    35821 ctatcgtcga caacttctat ctcgtcgcct tgatcttcgc gatgggtacc agtctgggcc
    35881 ttgccacgcc gctggtgacc gagtgtatgc aatggttgtt tggcattccg cataccctgc
    35941 aactggacgc tatcatcatt acctgctgga ttatcctcaa cgccatttgc gtcgcttgcg
    36001 gtctgcaaaa aggggtacgt atcgccagtg acgtgcgtag ttacctgagc ttcctgatgc
    36061 tgggttgggt gttcattgtc agcggtgcca gcttcatcat gaactacttc accgattcgg
    36121 tggggatgtt gctgatgtat ctgccgcgca tgttgttcta taccgatccc atcgctaaag
    36181 gcggcttccc gcagggctgg accgtgttct actgggcatg gtgggtgatt tatgctatcc
    36241 agatgagtat cttcctcgcc cgcatctccc gtggtcgtac tgtgcgtgaa ctgtgcttcg
    36301 gcatggtgct ggggctgaca gcgtcaacct ggatcctgtg gactgtactc ggtagtaaca
    36361 ctctgctgtt gatagataaa aacatcatca acattccaaa tctgatcgaa cagtacggtg
    36421 tggcgcgcgc catcattgaa acctgggccg ctctgccact cagcaccgcc accatgtggg
    36481 gcttcttcat cctctgcttt attgccaccg ttacgctggt taacgcctgc tcttataccc
    36541 tggcgatgtc cacttgccgc gaagtacgcg atggtgaaga accacctctg ctggtgcgta
    36601 tcggttggtc aattctggtt ggcattatcg gtattgttct gctggcgctc ggcggcctga
    36661 aaccgattca aaccgccatt atcgccggag gatgcccgct gttcttcgtc aacattatgg
    36721 tgacgctctc ctttattaaa gacgcgaaac agaactggaa agattaatta acccccaaaa
    36781 tatcaagagg ttgaaagatg gattttaatt taaatgatga gcaggaactg tttgtcgccg
    36841 gtatccgcga actgatggcc agcgaaaact gggaggccta ttttgccgag tgcgaccgtg
    36901 acagcgtcta cccggaacgt tttgtcaaag cactggcgga tatgggtatc gacagtctgc
    36961 tgatccctga agagcacggt ggtctggacg cggggtttgt tactctcgcc gccgtgtgga
    37021 tggagctggg acgtctgggg gcaccaacct atgtgctgta ccagttgccg ggcgggttca
    37081 acaccttcct gcgcgaaggc acacaagagc agatcgacaa aattatggct ttccgcggca
    37141 ccggtaagca gatgtggaac tcagcgatta ccgaaccggg cgcgggctcc gacgtgggta
    37201 gcctgaaaac gacttatacc cgtagaaatg gtaagattta tcttaatggt agtaagtgtt
    37261 ttattaccag cagcgcctac accccgtaca tcgtggtgat ggcgcgcgac ggggcttctc
    37321 cggacaaacc tgtctacacc gaatggtttg ttgatatgag caaaccgggc atcaaagtga
    37381 ccaaacttga aaagctcggt ctgcgtatgg atagctgctg tgaaatcacc tttgacgacg
    37441 tggaactgga cgagaaagac atgttcggtc gggaaggtaa cggctttaac cgcgtcaaag
    37501 aagagttcga ccatgaacgt ttcctggtag ccctcaccaa ctacggtacg gcgatgtgcg
    37561 cctttgaaga tgcggcgcgc tacgccaatc agcgcgtgca gtttggcgag gctattggtc
    37621 gtttccagtt gattcaggaa aaattcgccc acatggcgat caaattaaac tccatgaaaa
    37681 acatgctgta tgaagcagcg tggaaagcag acaacggcac catcacctct ggcgatgcag
    37741 cgatgtgcaa atacttctgc gccaatgcgg catttgaagt tgtggatagc gcaatgcagg
    37801 tgctgggcgg tgtcgggatt gcgggcaacc accgcatcag ccgcttctgg cgtgatctgc
    37861 gtgtagaccg cgtctccggg ggatctgacg aaatgcagat cctgacgctg ggtcgcgcgg
    37921 tgctgaagca ataccgctaa gtccgtgcgc tgcctgatgc gacgctaatg cgtcttatca
    37981 ggcctacggg agcacgaatg taggccggat aaagcgttta cgccgcatcc ggcagtcatg
    38041 cgctacataa atttttcagg agctaataat ggatcatcta cccatgccga aattcgggcc
    38101 gttggccgga ttgcgcgttg tcttctccgg tatcgaaatc gccgggccgt tcgccgggca
    38161 aatgttcgca gaatggggtg cggaagttat ctggatcgag aacgtcgcct aggccgacac
    38221 cattcgcgtt caaccgaact acccgcagct ctcccgccgc aatttgcacg cgctgtcgtt
    38281 aaatattttc aaagatgaag gccgcgaagc gtttctgaaa ttaatggaaa ccaccgatat
    38341 cttcatcgaa gccagcaaag gtccggcctt tgcccgtcgt ggcattaccg atgaagtact
    38401 gtggcagcac aacccgaaac tggttatcgc tcacctgtcc ggttttggtc agtacggcac
    38461 cgaggagtac accaatcttc cggcctataa caccatcgcc caggccttca gtggttacct
    38521 gattcagaac ggtgatgttg accagccaat gcctgccttc ccgtataccg ccgattactt
    38581 ttctggcctg accgccacca cggcggcgct ggcagcactg cataaagtgc gtgaaaccgg
    38641 taaaggcgaa agtatcgaca tcgccatgta tgaagtgatg ctgcgtatgg gccagtactt
    38701 catgatggat tacttcaacg gcggcgaaat gtgcccgcgc atgagcaaag gtaaagatcc
    38761 ctactacgcc ggttgcggtc tgtataaatg cgccgacggc tacatcgtga tggaactggt
    38821 gggcattacc caaattgaag agtgctttaa agatattggc ctcgcacatc tactcggtac
    38881 gccggaaatc ccggaaggca ctcagcttat ccaccgtatc gaatgccctt acggcccact
    38941 ggtagaagaa aaactcgatg cctggctggc ggcacatacc atcgcagaag ttaaagaacg
    39001 ctttgccgaa ctgaatatcg cctgtgccaa agtactgacc gtaccggaac tggaaagcaa
    39061 tccacagtat gttgcccgtg aatcaatcac tcagtggcaa acgatggatg gtcgcacctg
    39121 caaagggccg aacatcatgc cgaaattcaa aaataacccc ggacaaatct ggcgcggaat
    39181 gccctcacat ggcatggaca cggctgccat tttgaaaaat atcggctaca gcgaaaacga
    39241 cattcaggag ttggtcagca aaggtctggc caaagttgag gactaaacgc gtggcgtcag
    39301 tcggcttgcc tgactagcgc ccgcccaata acaaacgaac aaaaatggat agaggtgcaa
    39361 tggatatcat tggcggacaa catctacgtc aaatgtggga cgatctggcg gacgtttacg
    39421 gtcataaaac ggcgctgatt tgtgaatcca gcggcggagt cgttaaccgg tatagttatc
    39481 ttgagttaaa tcaggagatt aaccgcacga caaacctgtt ttatacgctg gggattcgca
    39541 aaggcgacaa ggttgctcta catctcgaca actgcccgga atttatcttt tgctggttcg
    39601 ggctggcaaa aattggcgcg attatggtgc cgattaacgc ccgcctgttg cgcgaggaaa
    39661 gcgcgtggat cctgcaaaat agccaggcgt gcctgctggt gaccagtgcg caattctatc
    39721 ctatgtatca acagattcag caggaagatg ccactcaatt gcggcacatt tgcctgacag
    39781 atgtggcact tcccgctgat gatggcgtga gttcgtttac tcaactgaaa aatcaacaac
    39841 ctgccacctt gtgctatgca ccgccgctat cgactgacga tacggcggaa attctcttca
    39901 cctccggcac cacctcccga ccgaaaggtg tggtgattac ccattacaac ctgcgcttcg
    39961 ctggatatta ctccgcctgg cagtgtgcac tgcgtgacga tgacgtctac ctgacggtaa
    40021 tgcctgcgtt tcatatcgat tgccagtgta ctgcggcgat ggcggcgttt tctgccgggg
    40081 ccacctttgt gctggtcgag aaatacagcg cccgcgcctt ctggggacag gtacagaagt
    40141 accgcgccac cgttaccgaa tgtattccga tgatgatccg taccttgatg gtgcagccgc
    40201 cttcagcgaa cgatcggcaa caccgcctgc gggaagtgat gttttatctc aacttgtcgg
    40261 agcaggaaaa agatgcgttt tgtgaacgct tcggcgttcg cttgctgacg tcttatggga
    40321 tgacggaaac cattgtgggc attatcggcg atcgccctgg cgataaacga cgctggccgt
    40381 cgattggtcg ggcggggttt tgctacgaag cggagatccg cgacgatcac aatcgcccgc
    40441 tcccggcagg tgagatcggt gaaatctgta ttaaaggcgt accagggaaa accatcttca
    40501 aagagtattt tctcaacccg aaagccactg cgaaagtgct ggaagccgac ggctggctac
    40561 ataccggcga taccggatac tgcgacgaag agggcttttt ttatttcgtc gatcgccgct
    40621 gcaatatgat caaacgcggt ggcgagaatg tctcctgcgt ggagctggaa aatatcatcg
    40681 ccacccatcc aaaaattcag gatatcgtgg ttgtgggtat taaagattcg attcgcgatg
    40741 aagccatcaa agcatttgtg gtgctgaatg aaggtgaaac attgagcgaa gaggaatttt
    40801 tccgcttctg cgaacaaaat atggcgaaat ttaaagtgcc ctcttatctg gagatcagaa
    40861 aagatctgcc acgtaattgc tcggggaaaa taattagaaa gaatctgaaa taaatgaaac
    40921 agcagggaac taccctgcca actaataatc acgctataaa acaatatgca ttttttgcag
    40981 ggatgttgtc gtccctgaaa aagcaaaaat ggagaaaagg aatgagtgaa tcattacatc
    41041 tgacccgcaa tggatcaatt ctggaaatta cccttgatcg tccaaaagcg aatgctattg
    41101 atgcaaaaac cagctttgaa atgggcgaag tatttctaaa tttccgtgac gatccgcaat
    41161 tacgtgtcgc cattattacc ggtgccggag agaagttctt ttccgcgggc tgggatttaa
    41221 aagcggcagc agaaggcgaa gcaccggatg ctgactttgg cccgggtgga tttgcgggat
    41281 taaccgaaat tttcaatctc gacaaaccgg ttatcgcagc tgtgaacggc tatgcctttg
    41341 gcggcggttt cgaactggcg ctggcggcag attttattgt ttgtgccgat aacgccagct
    41401 tcgccctgcc ggaagccaag ctgggcatcg ttcctgacag cggcggcgtg ctgcgtctac
    41461 cgaagatcct gccgcctgcc atcgtcaatg aaatggtgat gaccggcaga cgaatgggcg
    41521 cagaagaggc gctgcgttgg gggatagtca accgcgtggt tagccaggcg gaactgatgg
    41581 ataacgcccg cgaactggcg cagcagctgg ttaacagcgc cccgctggcg attgcggcgc
    41641 tgaaagagat cttccgcacc accagcgaaa tgccggtaga agaagcgtat cgctatattc
    41701 gcagcggcgt gttgaaacac tatccgtcgg ttctgcattc ggaggatgcc attgaagggc
    41761 cgctggcgtt tgccgagaag cgcgatccgg tgtggaaagg acgttaacaa ccgtgagcta
    41821 ttacgccttt gaggggttaa ttccggtggt tcacccgacg gcgtttgtcc atcccagtgc
    41881 cgtcttgatt ggcgatgtga ttgtgggagc cggtgtctac atcggcccac tcgcctcact
    41941 gcgtggtgac tacgggcggt tgatcgtgca agcgggagcc aatattcagg atggctgcat
    42001 tatgcatggc tactgcgaca ctgacaccat cgttggggaa aacggccata tcgggcacgg
    42061 agcgatcctg catggttgtg tgattggtcg cgatgcattg gtcgggatga acagcgtgat
    42121 tatggatggc gcggtcattg gcgaagagag cattgttgcc gccatgagct ttgtcaaagc
    42181 aggctttcac ggcgagaaac gccagttgtt gatgggtacg ccagcccgcg ctgtacgcag
    42241 tgttagtgac gacgagttac actggaaacg gttgaatacc aaagagtatc aggatcttgt
    42301 cgggcgctgt catgcatcgt tacatgaaac acagccactg aggcaaatgg aggaaaatcg
    42361 cccccgtttg caggggacga cggatgtgac gcctaaacgg tgatcttgcc ggatgcggcg
    42421 taaacgcctt atccaactct gctggagagc aaccgtaggc cggataagat gcgccagcat
    42481 cgcatccggc gatagctgtc ggatgcggca taaacgcctt atccaacccg gctggagagc
    42541 aaccgtaggc tggataagat gcgccagcat tgcatccagc aaccaggctg cgtggtatca
    42601 acgaaatgac attaacgacg catactcttt gacaacatca tctgccactt ctgctcgcgg
    42661 ttaagttccg tgagcggaac ggcaggaata cgcggcgtct ttcttacgcc gactaattta
    42721 tcccggctgt tattgcgcag gcgcgcgtaa atttgctcat cgatatcgac cacttttacc
    42781 agtcgctggc actggcatcc ccgtccttcc agcttattag ggatcatctt aactgcgcag
    42841 cttatttctg cgacttccga cagaatataa gtcagggtat taaccgcctt gctgtgttca
    42901 atatcgaaat gaatagagat ctctcttgct atcacccacc gattttcagc catcatccat
    42961 tcggcgatta acaagtagag tggtttttca acatatcctt cacacatacg aactccagtt
    43021 tcacaaatta aaagtctggc tatgactcgt tttgaagcaa ttaaacaagg ccatattaaa
    43081 attgtggata tatcgatagt atgcaacttc accgtcgata a
//
LOCUS       DAKISA010000025        43049 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.025, whole genome shotgun sequence.
ACCESSION   DAKISA010000025 DAKISA010000000
VERSION     DAKISA010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43049
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.025"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..987
                     /gene="gldA"
                     /locus_tag="OVW45_001413"
     CDS             <1..987
                     /gene="gldA"
                     /locus_tag="OVW45_001413"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HCV5525906.1"
                     /translation="VLGFAQSTVEKSFKDAGLVVEIAPFGGGCSQNEIDRLRGIAETA
                     QCGAILGIGGGKTLDTAKALAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYL
                     LLPNNPNMVIVDTKIVAGAPARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAA
                     LALAELCYNTLLEEGEKAMLAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVH
                     NGLTAIPDAHHYYHGEKVAFGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIK
                     EDVPAKMRIVAEAACAECETIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            1261..1878
                     /locus_tag="OVW45_001414"
     CDS             1261..1878
                     /locus_tag="OVW45_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HCV5525907.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQKRGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTHHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(1905..2810)
                     /gene="yijE"
                     /locus_tag="OVW45_001415"
     CDS             complement(1905..2810)
                     /gene="yijE"
                     /locus_tag="OVW45_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCV5525908.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVMALALVSRKKKEAVSVKRI"
     gene            complement(2904..5084)
                     /gene="katG"
                     /locus_tag="OVW45_001416"
     CDS             complement(2904..5084)
                     /gene="katG"
                     /locus_tag="OVW45_001416"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCV5525909.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSERVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(5413..6303)
                     /gene="metF"
                     /locus_tag="OVW45_001417"
     CDS             complement(5413..6303)
                     /gene="metF"
                     /locus_tag="OVW45_001417"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCV5525910.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(6652..9084)
                     /gene="metL"
                     /locus_tag="OVW45_001418"
     CDS             complement(6652..9084)
                     /gene="metL"
                     /locus_tag="OVW45_001418"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCV5525911.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTDFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(9087..10247)
                     /gene="metB"
                     /locus_tag="OVW45_001419"
     CDS             complement(9087..10247)
                     /gene="metB"
                     /locus_tag="OVW45_001419"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCV5525912.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEVRAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            10524..10841
                     /gene="metJ"
                     /locus_tag="OVW45_001420"
     CDS             10524..10841
                     /gene="metJ"
                     /locus_tag="OVW45_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCV5525913.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            11025..11633
                     /locus_tag="OVW45_001421"
     CDS             11025..11633
                     /locus_tag="OVW45_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCV5525914.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            11778..12453
                     /locus_tag="OVW45_001422"
                     /pseudo
     CDS             11778..12453
                     /locus_tag="OVW45_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(12626..12862)
                     /locus_tag="OVW45_001423"
     CDS             complement(12626..12862)
                     /locus_tag="OVW45_001423"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525915.1"
                     /translation="MKAIQCCYFLALVSSHLMAGVMGDDNIFLSNSDSAALYCVAKIF
                     PDGENILIYKVNIINASSKKDVFFMKAQHQPTMT"
     gene            complement(12864..13544)
                     /locus_tag="OVW45_001424"
                     /pseudo
     CDS             complement(12864..13544)
                     /locus_tag="OVW45_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
     gene            complement(13518..13808)
                     /locus_tag="OVW45_001425"
                     /pseudo
     CDS             complement(13518..13808)
                     /locus_tag="OVW45_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            complement(13869..14666)
                     /gene="istB"
                     /locus_tag="OVW45_001426"
     CDS             complement(13869..14666)
                     /gene="istB"
                     /locus_tag="OVW45_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
                     /protein_id="HCV5525916.1"
                     /translation="MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQ
                     EWNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPGIDRKVVRELAGLAFVERSENVIL
                     LGPPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYAR
                     VLILDEIGYLPMNREEASLFFRLLNRRYEKASIILTSNKGFADWGEMFGDHVLATAIL
                     DRLLHHSTTLNIKGESYRLKEKRKAGVLTKNTTPISDDEMVESGQHQ"
     gene            complement(14666..15838)
                     /gene="istA"
                     /locus_tag="OVW45_001427"
     CDS             complement(14666..15838)
                     /gene="istA"
                     /locus_tag="OVW45_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family transposase"
                     /protein_id="HCV5525917.1"
                     /translation="MLSREDFYMIKQMRQQGAYIVDIATQIGCSERTVRRYLKYPEPP
                     ARKTRHKMVKLKPFMDYIDMRLAENVWNSEVIFAEIKAMGYTGGRSMLRYYIQPKRKM
                     RPSKRTVRFETQPGYQLQHDWGEVEVEVAGQRCKVNFAVNTLGFSRSFHVFAAPKQDA
                     EHTYESLVRAFRYFGGCVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRAC
                     RPRRARTKGKVERMVKYLKENFFVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKET
                     PEQRFALEQEHLQPLPDTDFDTSYFDIRHVSWDSYIEVGGNRYSVPEALCGQPVSIRI
                     SLDDELRIYSNEKLVASHRLCSASSGWQTVPEHHAPLWQQVSQVEHRPLSAYEELL"
     gene            16144..16220
                     /locus_tag="OVW45_001428"
     tRNA            16144..16220
                     /locus_tag="OVW45_001428"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16178..16180,aa:Met,seq:cat)
     gene            16427..16879
                     /gene="rimP"
                     /locus_tag="OVW45_001429"
     CDS             16427..16879
                     /gene="rimP"
                     /locus_tag="OVW45_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCV5525918.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            16907..18394
                     /gene="nusA"
                     /locus_tag="OVW45_001430"
     CDS             16907..18394
                     /gene="nusA"
                     /locus_tag="OVW45_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCV5525919.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            18419..21091
                     /gene="infB"
                     /locus_tag="OVW45_001431"
     CDS             18419..21091
                     /gene="infB"
                     /locus_tag="OVW45_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCV5525920.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEAHRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            21255..21656
                     /gene="rbfA"
                     /locus_tag="OVW45_001432"
     CDS             21255..21656
                     /gene="rbfA"
                     /locus_tag="OVW45_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCV5525921.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            21656..22600
                     /gene="truB"
                     /locus_tag="OVW45_001433"
     CDS             21656..22600
                     /gene="truB"
                     /locus_tag="OVW45_001433"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCV5525922.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            22749..23018
                     /gene="rpsO"
                     /locus_tag="OVW45_001434"
     CDS             22749..23018
                     /gene="rpsO"
                     /locus_tag="OVW45_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCV5525923.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            23265..25400
                     /gene="pnp"
                     /locus_tag="OVW45_001435"
     CDS             23265..25400
                     /gene="pnp"
                     /locus_tag="OVW45_001435"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCV5525924.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            25509..26393
                     /gene="nlpI"
                     /locus_tag="OVW45_001436"
     CDS             25509..26393
                     /gene="nlpI"
                     /locus_tag="OVW45_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCV5525925.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            26500..26580
                     /gene="yrbN"
                     /locus_tag="OVW45_001437"
     CDS             26500..26580
                     /gene="yrbN"
                     /locus_tag="OVW45_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCV5525926.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            26573..28462
                     /gene="deaD"
                     /locus_tag="OVW45_001438"
     CDS             26573..28462
                     /gene="deaD"
                     /locus_tag="OVW45_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCV5525927.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            28616..29860
                     /gene="mtr"
                     /locus_tag="OVW45_001439"
     CDS             28616..29860
                     /gene="mtr"
                     /locus_tag="OVW45_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCV5525928.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(29978..30985)
                     /locus_tag="OVW45_001440"
     CDS             complement(29978..30985)
                     /locus_tag="OVW45_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HCV5525929.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(31191..32069)
                     /locus_tag="OVW45_001441"
     CDS             complement(31191..32069)
                     /locus_tag="OVW45_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCV5525930.1"
                     /translation="MKYSLGPVLWYWPKEALEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            complement(32078..33073)
                     /locus_tag="OVW45_001442"
     CDS             complement(32078..33073)
                     /locus_tag="OVW45_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCV5525931.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            33282..33806
                     /locus_tag="OVW45_001443"
     CDS             33282..33806
                     /locus_tag="OVW45_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCV5525932.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            33800..34303
                     /locus_tag="OVW45_001444"
     CDS             33800..34303
                     /locus_tag="OVW45_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCV5525933.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(34290..34592)
                     /gene="yhbQ"
                     /locus_tag="OVW45_001445"
     CDS             complement(34290..34592)
                     /gene="yhbQ"
                     /locus_tag="OVW45_001445"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCV5525934.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            34643..35086
                     /locus_tag="OVW45_001446"
     CDS             34643..35086
                     /locus_tag="OVW45_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCV5525935.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            complement(35066..35584)
                     /gene="yhbO"
                     /locus_tag="OVW45_001447"
     CDS             complement(35066..35584)
                     /gene="yhbO"
                     /locus_tag="OVW45_001447"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCV5525936.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            35712..36347
                     /locus_tag="OVW45_001448"
     CDS             35712..36347
                     /locus_tag="OVW45_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCV5525937.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            36420..37460
                     /locus_tag="OVW45_001449"
     CDS             36420..37460
                     /locus_tag="OVW45_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCV5525938.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(37574..38149)
                     /gene="yraP"
                     /locus_tag="OVW45_001450"
     CDS             complement(37574..38149)
                     /gene="yraP"
                     /locus_tag="OVW45_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCV5525939.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(38159..38749)
                     /gene="diaA"
                     /locus_tag="OVW45_001451"
     CDS             complement(38159..38749)
                     /gene="diaA"
                     /locus_tag="OVW45_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCV5525940.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            complement(38769..39164)
                     /locus_tag="OVW45_001452"
     CDS             complement(38769..39164)
                     /locus_tag="OVW45_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCV5525941.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            complement(39122..41158)
                     /gene="lpoA"
                     /locus_tag="OVW45_001453"
     CDS             complement(39122..41158)
                     /gene="lpoA"
                     /locus_tag="OVW45_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCV5525942.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKKQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAML
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPVS"
     gene            41222..42082
                     /gene="rsmI"
                     /locus_tag="OVW45_001454"
     CDS             41222..42082
                     /gene="rsmI"
                     /locus_tag="OVW45_001454"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCV5525943.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            complement(42125..>43049)
                     /locus_tag="OVW45_001455"
     CDS             complement(42125..>43049)
                     /locus_tag="OVW45_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCV5525944.1"
                     /translation="TSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKL
                     NDSLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPV
                     NIGANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNL
                     TAPQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQ
                     MRIDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGT
                     VNMRAWPVNLVGGELETGKFQGTATITVMVR"
BASE COUNT        10175 a        11190 c        11448 g        10236 t
ORIGIN      
        1 gttttaggtt ttgctcaatc cactgtcgag aaaagcttta aagatgctgg actggtagta
       61 gaaattgcgc cgtttggcgg tggatgttcg caaaatgaga tcgaccgtct gcgtggcatc
      121 gcggagactg cgcagtgtgg cgcaattctc ggtatcggtg gcggaaaaac cctcgataca
      181 gccaaagcac tggcacattt catgggtgtt ccggtagcga tcgcaccgac tatcgcctct
      241 accgatgcac cgtgcagcgc attgtctgtt atctacaccg atgagggtga gtttgaccgc
      301 tatctgctgt tgccaaataa cccgaatatg gtcattgtcg acaccaaaat cgtcgctggc
      361 gcacctgcac gtctgttagc ggcgggtatc ggcgatgcgc tggcaacctg gtttgaagcg
      421 cgtgcctgct ctcgtagcgg cgcgaccacc atggcgggcg gcaagtgcac ccaggctgcg
      481 ctggcactgg ctgaactgtg ctacaacacc ctgctggaag aaggcgaaaa agcgatgctt
      541 gctgccgaac agcatgtagt gactccggcg ctggagcgcg tgattgaagc gaacacctat
      601 ttgagcggtg ttggttttga aagtggtggt ctggctgcgg cgcacgcagt gcataacggc
      661 ctgaccgcta tcccggacgc gcatcactat tatcacggtg aaaaagtggc atttggtacg
      721 ctgacgcagc tggttctgga aaacgcaccg gttgaggaaa tcgaaaccgt agctgcactt
      781 agccatgcgg taggtttgcc aataactctc gctcaactgg atattaaaga agatgtcccg
      841 gcgaaaatgc gaattgtggc tgaagcggca tgtgcagaat gtgaaaccat ccacaacatg
      901 cctggcggcg cgacgccaga tcaggtttac gccgctctgc tggtagccga ccagtacggt
      961 cagcgtttcc tgcaagagtg ggaataacct actccaaact cccggcttgt ccgggagttt
     1021 gaacgcaaaa ttgcctgatg cgctacgctt atcaggccta cgcaatctct gcaatatatt
     1081 gaattttctt gttttttagg ccagataagg cgttcacgcc gcatccggca ctctgtgcca
     1141 gtaatctgaa actcccacgt tgttcaggag ttttttatcc gcttctggac attttctcta
     1201 cagaacaatc gttatcccgg aaggtcgaat ctgtgactct tattttcacg gacgaaccag
     1261 atgaaagctt cactggcgct gctcagcctg ctaacggcat ttaccagcca ctccttaaaa
     1321 tctcccgccg ttccaccaac cgtggtacaa atccaggcca ataccaacct tgccatcgca
     1381 gacggtgcca gacagcaaat cggcagcacg ctattttacg atccggcgta tatgcagctt
     1441 acctatcctg gcggtgatgt cccgcaaaaa cgcggggtat gttccgatgt ggtgatccgt
     1501 gcattgcgca gccagaaggt cgatttgcag aaactggtgc acgaagatat ggcgaagaat
     1561 tttgccgagt acccgcaaaa atggcagtta aagcgcccgg acagcaacat cgatcaccgt
     1621 cgggtgccta atctggaaac ctggtttacc caccacgata aaacacgccc catcagcaag
     1681 aaccccagtg actatcaagc gggcgatatt gtctcctggc gattagataa cgggctggca
     1741 catattgggg tggtttcaga tggcttcgcc cgcgacggta cgccgctggt gatacataac
     1801 attggcgcag gtgcgcagga agaagatgtg ctgtttagct ggcgcatggt ggggcattat
     1861 cgttattttg ttaaataagc gatcgcccca catgaagaaa aaattcagat cctttttaca
     1921 ctaacggctt cttttttctt acggctcacc agcgccagtg ccatcacaat cagcacaata
     1981 ccgctacctt caacggcccc cggattctcg ccgagcagcc accaggaaaa gagtacgccg
     2041 caaacgggaa cggccagtgt gcttaagctg gcaatactgg caggcaagtt tttcaataca
     2101 aacaaccata agctccacgc cagtgccgtc gccagaatcg cactgtaggc cagcgcccag
     2161 aacacggtgg gctgccagtc aatttcacgt tgcggcacca gtaaagcgac cacactcatc
     2221 accagcgccg catagagcat ctgccaggat gttaacgaca ataaatccac gcgcggatga
     2281 cgggcataca gacgtttagc aacaatcgcg ctcgcccccc aactgacgcc ggagaggatt
     2341 gccagcatgg cacttttcat cgaagagaaa tcgagttgcc acggctgcaa caccaaaaat
     2401 aagccgaaag cggcaatcag tatcgcgaaa tattgcccac gtcgcaggcg ttcaccgaga
     2461 aacaacgcgg cgaaaatcac cacccagaac ggcatggtat agcttaggat cgccaccttc
     2521 cccgctccgc cgctgaccag cgcccactgc gccaggccaa ccatcccgca ggtttgtaac
     2581 agggcaatgg ctaaggtgta tttaaacggt gtcgggcgca ttccgcgacc acgtaataaa
     2641 aggacgatga ataaaacgag agcgccgaaa atgcagcgta aggcggtaaa gtcgaaggca
     2701 ccgatgtagc tggtgacttg cttcatgaaa atccagctat aactccagat aagtgtgagc
     2761 acaaccaggc cactgattgc cagtgggttg ctctttcctg cggcagacat gagagatcca
     2821 gtgtgtagtt ttcctgtgga cactatacgc cggtaaagaa acttcagcga ctgcctgaaa
     2881 ggcagtcgct gaagaaggtc agattacagc aggtcgaaac ggtcgaggtt catcactttc
     2941 acccatgccg ccacgaagtc tttaacaaac ttctcgtgag catcgctgct ggcgtaaact
     3001 tcagccaccg cacgcaggac agagttagaa ccaaatacca gatccgcacg gctggcagtg
     3061 tatttcactt cgccggtttc gcggtcacgg ccttcaaaca gctctttcga ttcgtcggtc
     3121 gctttccact cgtaacgcat atccagcaag ttcacgaaga agtcattgct caatacgcca
     3181 acgcggtcag tgaagacgcc gtttttgctg ccatcgaagt tggcacccag tacgcgcatc
     3241 ccgcccacca gcgcggtcat ttccggcgcg gtcagggtca gttgctgggc tttgtcgatc
     3301 agcagtgact cggtggtgga aacatccaga cgagcgcgat agttacggaa accgtcagca
     3361 attggctcca gcagctcgaa catctcaatg tcagtctgat cctgacgcgc atcaacgcgg
     3421 cccggcgcaa acggtacatg aatgctcaaa cctgcggcgc ttgcggcttt ctcaacacca
     3481 accacaccag ccagcacgat gatatccgcc agcgaggctt taccagactc tttctggatt
     3541 ttctccagaa caggcagagc acgaacggct gcggcgttca catcccagtc gcgctgcggc
     3601 attaatgcca gacgcgcacc gttggcacca ccgcgtttgt cgccaccacg gaaggtagaa
     3661 gcagatgccc aggccaccga tacccgctca ctaacagaca gaccagaatc cgcaatcgcg
     3721 aatttcagat cgataatgtc ctgctcggtc gggttgtaga tcggctgcgg cagcggatct
     3781 tgccagatca gatcttcttt cggcacttcc ggcccgatgt agcgagattt cggccccata
     3841 tccctgtgcg tcagtttgaa ccaggcacgg gcaaaggctt cgttgaacgc ctgcggatcg
     3901 ttgaggaaac gacgagagat cttctcgaac tcaggatcaa aacgcagcgt caggtcggtc
     3961 accagcatcg tcggtttacg tttcttcgac ggatcgaatg gatccgggat aatttccggt
     4021 gcgtctaccg cttcgaactg gattgcgcca gccggactgc gggtctgtac ccactcatat
     4081 ttgaacaggt tttcgaagaa atagttgctc cactgggtcg gcgtctgggt ccagacaact
     4141 tccagaccag aggtaatggc atctgcgcca acgccgctgc cgtaagtgct cgcccagcct
     4201 aaaccttgtt cttcaatcgg tgcagcttct ggatcaggac ctacgtttga tgtcggaccg
     4261 gcaccgtggg ttttacccag cgtatgacca cccgcaatca gcgccacggt ttcttcgtcg
     4321 ttcatgccca tgttgccgaa ggtcgcgcgg atagctgctg ccgcagaaag cggttcgccg
     4381 ctgtgatccg ggccttccgg gttaacgtaa atcaggccca tctcggttgc acccagcggt
     4441 gctttcgcca gcgcttccgg atgacggtga gtcagccagg ctttttcatc accccagtta
     4501 acatccagat ccggttccca gacgtcttca cgaccggcac caaaaccgaa ggtacggaag
     4561 ccggagtttt ccagcgccac gttacctgcg aggataaaca ggtcggccca ggagattttc
     4621 tgaccatatt tctgtttgat tggccacaac aggcgacgcg ctttatcgag gcttacgtta
     4681 tccggccagg agttcagcgg tgcaaaacgt tgctgaccac gacccgcgcc gccgcgtcca
     4741 tcgattgaac ggtaagtacc tgcgccgtgc caggccatac gaataaacag accggcgtag
     4801 ctgccccagt cggctggcca ccacggctga gattctgtca gcagggcttt cagatctttt
     4861 ttcaggccgt agtaatctaa tttgctgaat tctttgcggt agtcaaagtc ctcacccagc
     4921 gggttagaac gattagaatg ttggtttaac aggtcaacac ggagttgatt tggccaccag
     4981 tcgcgagtgg ttgtgcccgc ccccgcgctc tggtcgtgac cgccctgatg gaacgggcat
     5041 ttgccagtgg ctgtcgtgtt atggatatcg tctgacgtgc tcatcaatgt gctcccctct
     5101 acagtgttac cgttacgata cacagcgtta gagagaagtt ataattgaat taatccacgg
     5161 atgcgatagt tgagatctta catattgttg gttaaagaga tgtaggtcaa attgatcgta
     5221 attaagcttt tttgattatg tccgaattcg gacaaatgct ttataaaaaa gggttttagc
     5281 ttcatatcct tcaagtaatg agggagaaaa caaggctgac aaccttattt tcccgccctc
     5341 atttcgaggc agcattttgt gctctgttta aaatttgtga tcactgtgtg attttcacaa
     5401 aagccacact atttataaac caggtcgaac acccagcgta tggcaaatcg cgtaactcat
     5461 ttcagcgcgg ttaagcgtat agaagtggaa atctttcact ccttcacggc ttaaaatctt
     5521 caccatatcc atggcaatat tcgcgcccac cagtttgcgg gtttcggcat catcatccag
     5581 gccgtcgaac atttgcgcca tccacgccgg aatacgcacg ttggtcatat cggcaaattt
     5641 cttcgcctgt ttaaagttag ataccggcaa aattcccgga ataatttcca catcaatgcc
     5701 cgccgataca cagcggtcac gaaaacgcag gtagctttcg acatcgaaga agaactgagt
     5761 aatcgcgcgg ttggctccgg catcgacttt gcgtttcaga ttaagcaaat ccgcctgagc
     5821 gctttttgct tccgggtgaa cttccggata cgccgccacg gagatatcga aatctgccac
     5881 ttcttttaat agcgtcacca ggtcagaagc atacatttct ggcttaccac ttcccggcgg
     5941 cagatcgcca cgcagcgcca cgatatgacg aataccgtta ttccagtagt cgcgtgcaat
     6001 ggtgcgcaac tcgtcgggcg tcgcatcaat gcaagtaaga tgcggtgccg cttccagacc
     6061 agtgcgatct ttaatgcctt taataatgct gtgcgtacgg tcgcgctcgc cggagttcgc
     6121 gccataggtc accgatacaa acttcggttt caggctgcta aggcgatcga tggagttcca
     6181 cagggtctgc tccatttcac tggtacgcgg cgggaaaaac tcgaacgaaa cgttaatctg
     6241 cccctggact tctgccaggc tctgattcag ggcatcccgc tggctggcgt gaaaaaagct
     6301 cataccttac ctcatcaatc gcttgtagtt atatgttgtg ttgtgcacat ctatccgttt
     6361 agacatccag atgtaaagat gaaggaaaag ccgaagggcg tcaaccgaaa aatgaccaat
     6421 gatggtgagg aaaaatcagg gaagatgaaa aaacttcagg atggtaagaa aaagaaaatc
     6481 cggtatgcaa agcaaaccgg actgcaaata taaactaacc atgtacttat tgcttcttgc
     6541 cggattcgtc gtaaacgctt aatccggcct aatcctatga taattcaata tatttcatta
     6601 gaaaccataa acccgaaaac atgagtaccg ggcattatta aatttctgaa attacaacaa
     6661 ctgtgccagc cggttgatat ccgactgaat cgccccggcg gtgacgtcgc gcccagcgcc
     6721 aggcccacgg atcaccagcg gattatcgcg ataccagcgg ctttcgatgg caaagacgtt
     6781 atcgcacggc agcagtgatg ccagcggatg gtcttcacgc accgcttcca cgcccacacg
     6841 cgctttgcca ttggcatcga aacgcgccac gtagcgcagc accagcccca tttcgcgggc
     6901 cgcttccagc cgttgcacca tctgctcgtt aagttcatcg ccattttcaa agaaatggtc
     6961 gatgctgccg ccttcgcaat gggcaggcac cagcgactcc acgcgtacct gatccggttc
     7021 gatgttgtaa cctgcttcac gcgccagaat caccagcttg cgcatcacgt ctttgccaga
     7081 gagatcgtca cgcgggtcag gttcggttaa gccctgctgc cacgcctgat ccaccagctc
     7141 ggtaaacggc acgctaccgt cgaattgcag gaacaaccag gagagcgtgc cggagaagat
     7201 cccgctgatc gacaaaatag tatcgccgct gtcgatcaga tcgcgcacgg tgtggttgat
     7261 cggtaagccc gcaccgacgg tggcattgta cagccagtga cgcccggttt tttcgaaggc
     7321 gtcgtggatc tggcgatatt tattgctgtc gctggctccc gccagtttgt tggcgctgat
     7381 gacgtggaaa ccgtggctgg cgaaatcaag atactgatca gcaagctgct ggctggcggt
     7441 aacgtccagc accactaaat catcatacgg atgggcgcgc atccacagga acaacgactc
     7501 ttcatcctgc tcaaccgctt catcgttgaa gaaggctaac gcgcggctgg cgtccagccc
     7561 gtcatagctc aacagactgc ggcggctgtc caccacgcct gccagcacaa attcaaagtc
     7621 ggtacgtgcc gaaagcgtgc tctgctcacg ggcgaacagt tccagccaac gggaaccgat
     7681 attgccctta ccgaacaata ccaggccgat gcgtttttct gcgcggaaga cggactgatg
     7741 cagcccctga atcaggcttt cggtcgggcc ggtgcgcagt actgccacca ggctgatgcc
     7801 gtcatcggac tgccaggtaa attcgaccgg ctggcctttc agttgctgcc agaagcggtg
     7861 gcaatgcagc gggttacggg tgacgcctgc accgaccatc gccaccagcg ccagcccctg
     7921 acgcaggcgc agttcgccag gtaatcccgc ttcgtcgagg attttcagcg cactgtcggc
     7981 cacttctgag gtgtagcaaa attgcagcaa ctggcgatcg ttatgtacgc caaccgccag
     8041 cgggcgtacc tgcgcgcgtt tcaggatttg gtcgatctct ttatgcgcca gtttgaaatc
     8101 ctgactggcg ggcacctgaa actcaatcaa acagacatca tcgtggctgg tgacaatacg
     8161 cgcaccagta ccggaggcca gcacgcgttc aatgcgcgtg gaaccttgat ccggcgtgta
     8221 gctacagcgc agttgcaggt cgatttcgct gccagaaacc ggctgtaaag tacgggcgtg
     8281 aagaacggga gccgccagac gagccagttc gctggcttca tccagacgca gcaacggcag
     8341 cagacaggca tctttcactt tacgcgggtc ggcactgtat accccggcga cgtcgctcca
     8401 gatggttaca cgagaaacac ccgccagcgc accgatttgt gtcgcggaat agtcggaacc
     8461 gttacgcccc agcagcaccg tttcaccggc gttgttacgg ctgataaacc cggtcaccac
     8521 cagacgtttg cccggatgtt gcaccagtag ctgttgcagc aacgggtaag aaagcccttc
     8581 atcaacctgc ggttgtgcgg cgcgttcagc gcgtaaaaac tcgcgggcat caagccaggc
     8641 cgctggcagc ccttgttgat taagtaccgc agacatcagg cgtgccgacc atacttcccc
     8701 gtggcccacc acttccgcat acactgcgtc gttaataccg ctgtcgagca gcgccgccag
     8761 gcgttccaga tcgctgacaa aagcgctaat gaggctatcg gcttcttcag cgggtagcag
     8821 accgctaatc agatcgcact gatagcgacg cagcgtttgt tgaacctgat gcgcagagag
     8881 acgatcggtc tggcttagtt tcaaccagtt aatcaactgg ttagtggtgc taccggcggc
     8941 ggaaaccacc atcatatcgt caggctgaga gtactccgcc ataatgcccg cgacacgcaa
     9001 ataacacttc acatcagcca gactactgcc accaaattta tgcagctgac gacctttcgc
     9061 ccctgcctgc gcaatcacac tcatttttac cccttgtttg cagcccggaa gccattttcc
     9121 aggtcggcaa ttaaatcttc gccatcttca ataccggtgg agatacgcag cagcgtctcg
     9181 gagatcccgg cggcagcacg cacttctggt gccatgcctg catgtgtcat ggttgcggcg
     9241 tgagagatta aactttccac tccccctaat gattccgcca gcgtaaacaa cgacagcccg
     9301 ctcaggaaac gacgcagcgt ctgctcatcg ccatccagtt caaaactcaa cattgcgcca
     9361 aagccttttt gctgacgcgc ggcaatttca tgcccctgat tttccggcag cgacgggtga
     9421 tacagttttt tcaccaacgg ctgggtttgc agatatttca caatcgcctg cgcgttgcgc
     9481 tgcgccagct ccatacgcgg caccagcgtt cgcaacccgc gtagcagcag atagctgtca
     9541 aacgcgccgc ccgtcacgcc aatattgttt gcccaccagg caagttcagt gacaacgtcc
     9601 gggtctttag caatcaccac gccagccacc acgtcagagt gaccgttcag atatttcgtg
     9661 catgaatgca acaccagatc ggcacctaat gccagcggat tttgtaatgc cgggcttaag
     9721 aaggtgttat ccaccacgct caccgccccg acttcccttg ccagatggca gattttcgca
     9781 atatccacga cgcgtaacaa tggattactt gggctttcta ccagtaccag tttgggtttt
     9841 tctgccagcg ctgcccgtaa tgcctgttca tcgccttgat caacaaacaa cacgcgatag
     9901 caaccgcgtt tcgccagact gtcgaacagg cgatagctac cgccgtagca gtcgtgcggc
     9961 gcaaccagca gatcgccagg tttcaaaaag acggtcgtta ccaggtgaat cgcggacatg
    10021 ccggtattag taagtactgc gccagcaccg ccttccagtt ctgccagcgc acgctgaacc
    10081 acatcgcgcg ttgggttgcc gcgacgcgag taatcatgcg cgcgcggttc attaaatccg
    10141 gtaaagttat aggtgctgga aagatggatg ggagggacaa cgcaaccata ctgttcgtcg
    10201 tcatttaacc cgctacgcac tgcgatggtg gcctgtttac gcgtcatgtg atgaagtttc
    10261 ctgggctttg tcggtgaaat gtcaggcacc agagtaaaca ttgtgttaat ggacgtcaat
    10321 gcatctggac atctaaactt ctttgcgtat agattgagca aatcccaaat agccgttaaa
    10381 attatatgca ttatcacgcc gacaggtgca ttacacgatg tcacggtaac gcctgtacgg
    10441 taaactatgc gggtttacgg tcagtaccca cataaattgt gtggtctggt ctcaatttat
    10501 tgacgaagag gattaagtat ctcatggctg aatggagcgg cgaatatatc agcccatacg
    10561 ctgagcacgg caagaagagt gaacaagtca aaaagattac ggtttccatt cctcttaagg
    10621 tgttaaaaat cctcaccgat gaacgcacgc gtcgtcaggt gaacaacctg cgtcacgcta
    10681 ccaacagcga gctgctgtgc gaagcgtttc tgcatgcctt taccgggcaa cctttgccgg
    10741 atgatgccga tctgcgtaaa gagcgcagcg acgaaatccc ggaagcggca aaagagatca
    10801 tgcgtgagat ggggattaac ccggagacgt gggaatacta aagcaaaaaa gagcggcgcg
    10861 gagtggaatc gcctgatgcg ctacgcttat caggcctacg tcatattgca atttattgaa
    10921 tttgcacgaa cttgtaggcc ggataaggcg ttcacgccgc atccggcata aacaacgagc
    10981 acgttgtctg cgacccaccg ctttttatac atggacgttt aactatgaaa aacaggctgc
    11041 tgatcctcag cctgctggtt tctgtacctg cctttgcctg gcagccacaa accggcgaca
    11101 tcatctttca gatctctcgc tcatcgcaaa gtaaagcgat ccaactggcg acccattccg
    11161 attatagcca caccggtatg ctggtgatac gcaacaaaaa gccctacgtt tttgaagcgg
    11221 tcggcccggt gaaatacacc ccgctcaagc agtggatcgc ccatggtgaa aagggcaaat
    11281 acgttgttcg ccgcgttgaa ggcggactga gtgttgaaca acagcaaaaa ctggcgcaaa
    11341 cggcaaaacg ttatctcggt aaaccgtatg atttcagctt ctcatggagc gacgatcgcc
    11401 agtactgttc ggaagtggtg tggaaggttt accagaacgc gctgggaatg cgcgtgggcg
    11461 agcagcagaa gttgaaagag ttcgatctca gcaacccgct ggtccaggct aagctcaaag
    11521 aacgctacgg caaaaatatt ccgcttgagg aaacggtagt ttcaccacaa gccgttttcg
    11581 acgcgccaca actcaccaca gtcgccaaag agtggccgct gttttcgtgg taatggatga
    11641 tgaaaaaaat aattcccgcc gtagtctgtt tgttgctttg tagttgcacg acctttccgc
    11701 aagctgtcgc tccggtaaac ggtgatgcct ccaattagtt gaattaagga tataatactc
    11761 gcttttaagg ttgtttcatg gtcacagtta accttcattg cccccgttgc cagtccgttc
    11821 aggtttaccg tcacgggcag aacccaaaaa gacatgagcg attccgcggc cgtgactgtc
    11881 accgggtatt tcagctcagt tactgttacg agccccgtaa accgggagtt aaagaccaaa
    11941 tcgctgaaat gactttcaac ggtgccgggg ttcgcgatac tgcccgtacg ctgaaaatcg
    12001 gcattaatac agtcatccga actttaaaaa actctcgcca cgaagaataa cgtcatcccc
    12061 ggtcgctcat gctgatgtgg cactcatctg caaacttagt cagcaatgga gctttgtcgg
    12121 cagtaaagcg cgacagcact ggctctggta cgtgtacaac acgaaaactg gtggtgttct
    12181 ggcctacact tttggccccc ggactgacga aacctgtcgt gaattgcggg cgctgcttac
    12241 ccttttacca tcggcatgtt gaccactgac gaatggggta gttacgctag agagttaccg
    12301 aaggagaagc atctgactgg taagattttt actcagcaca ttgagcgtaa caatctgacc
    12361 ctgagaaccc gaatcaaacg tctggcccgc agaaccatct gtttctcgcg ttctgtagaa
    12421 cttcatgaaa aagtcatcgg tgcttttatc gaaaatatat gttctactaa ttggagtcat
    12481 cacccttctg ataagctttc cttggatgat taccagactg aatacacagc caaagatttt
    12541 tacctcattc tgctcttttt tctcaaatga catatattat attttacatc atattcatag
    12601 gcatatgaca ttcccccgcc ccccattatg tcattgtggg ttgatgttgg gctttcataa
    12661 aaaaaacatc tttttttgaa ctagcattaa ttatatttac tttatatatt aaaatatttt
    12721 ctccatctgg aaatatttta gccacacaat ataaagcagc tgaatccgaa ttacttaaaa
    12781 atatattatc atcacccata actccagcca ttaaatgact ggagactaaa gcaaggaaat
    12841 aacaacattg tattgccttc attttaatta ctcccttgcc tgataattgt aactccattc
    12901 cctttagctt tgggattata ttttagatag gtgttatttg gtgttcccaa gtaactactg
    12961 tattcttttt ttcccattcc attcatataa gaattcgtta aatctttgct tgaaaaattt
    13021 agactatcat aaacatcatt ttcttttgtt actttatttc ctttatcgtc agagtaaaaa
    13081 ccgtgagtgt gataatcgcc tactctctct gaaccttcag ggcattttat atttcgcaag
    13141 tctacactat cattatcatt tgaacttatc ggattcatgg ggaaatattc accttgcttc
    13201 ttacatatta gtccaccata ttctttattc tcgttaattg attctccatt acacattgcc
    13261 aatgcaaata ttccaatatc atcaatattt tttgatttaa attcatataa tcctaatgga
    13321 tccatgctat tcaccgggct cagcggatat gtatacaggt tccatccccc cttcagccca
    13381 atcggatcct gagtgatata ccgcccctgc agcgggtcat aatagcggtg gcggttataa
    13441 tacaggccgg actcctcatc atactgctgc cccggcaaac gaataagctg ctgcagctga
    13501 tgcgggttct cttcattcag caggttgccc cattcatcat attcccggcg tatttccagc
    13561 ccgtcaggcc cggtggctga cgttaactgg ctgtggtggt tatagtaaaa cgccgatgcc
    13621 ctgccatccg gcgtggtgat gcgcgtgatg agacccgtca ccacatccgg gctgtattct
    13681 gttgtccggc ctgcggcatc cgtctgtgtc ctgagcctgc ccacggcgtc aaactgactc
    13741 tgcgtgacgc tgccgtccgc gtgttccttt ttcaccaccc gcttcagccc ggcttcaccc
    13801 tacgtgtgct gtcaacgcca cgatgtttga ccgttatttg ccattttcat gcccgctaaa
    13861 tactttcgtt actgatgctg tccgctttcc accatttcat catcactgat tggcgttgtg
    13921 tttttggtca gcactccagc tttacgtttc tcttttaacc ggtaactctc tcctttgata
    13981 ttcagcgtgg ttgagtgatg tagcagccga tccaggatcg ctgttgccag tacgtgatct
    14041 ccgaacattt ctccccagtc tgcgaaccct ttgtttgacg tcagtatgat gctcgctttt
    14101 tcatatcgac ggttcagtag ccggaagaac agactggctt cctctctgtt catcggcaga
    14161 tagcctattt catccaggat caacacccgg gcataactca gttgctgtag ctgacgctcc
    14221 agccggtttt cctgtttcgc tttcatcagt gtcgcgatca gtctgtccag tggcataaac
    14281 agtacccgat gtcccgcatc caccgctttc acgccaagag ctatggccag atgagttttt
    14341 ccgacaccag gagggcccag caggatcacg ttttcgctgc gctccacgaa cgccagacca
    14401 gccagttccc ggacaacctt acggtcgatg cccggctgga aggtaaagtc gaactgctcc
    14461 agcgttttga cccacggcag acgagcctgc ttcagcctgg actccatacc gcgctgatgc
    14521 ctgccgttcc attcctgctg tagcgccatg cacaggaact cccggtagtt cagctctttt
    14581 ttagctgcct gttccagcag gctttcaacg tgataactca gatgctccat tttcaggcga
    14641 ctcagcagga cttccagttc atgcatcaca acagctcctc ataggcactc agtggtcgat
    14701 gttccacctg actgacctgc tgccagagcg gggcgtgatg ctccggcact gtctgccagc
    14761 cagacgatgc tgaacagagg cgatgtgagg ccaccagttt ctcattactg tagatccgca
    14821 actcgtcatc cagcgatatt cgtatcgata ccggctgacc acacagcgct tcgggaacgc
    14881 tgtaacgatt accaccaacc tcgatatagc tgtcccagga cacatggcgg atatcgaagt
    14941 aactggtatc gaagtctgta tccggtaacg gctgcagatg ttcctgctcc agcgcgaagc
    15001 gctgttccgg cgtttctttg aactggcgaa gctcccgttt gtcagccaca tcggctatcc
    15061 attgctccag ttgttgattg acatgagtga agctgtcgaa cctgcggtac cggacgaaga
    15121 agttctcctt gaggtatttc accatccgct caaccttacc ttttgttctg gccctgcgtg
    15181 gacggcatgc ccgtggcagg aagttatagt ggtcagccag caacaggaat ccggagttga
    15241 acacgacttt cccgttgtta ttcttcagca ccgcagcctt ctggttatca accagcaccg
    15301 ttttcacaca accaccgaag tagcggaagg cgcgaaccag tgattcgtag gtatgctcag
    15361 catcctgttt tggtgcggcg aagacatgga agctgcggga gaaccccagc gtattaaccg
    15421 caaagttaac tttgcaccgt tgcccggcaa cctccacctc aacttcgccc cagtcatgct
    15481 ggagctggta tccaggctga gtttcgaagc gaactgttct ttttgacgga cgcattttac
    15541 gtttgggctg gatgtagtaa cgcagcatgg aacgtccgcc cgtataaccc attgccttaa
    15601 tctccgcgaa gataacctcg ctattccaga cattctctgc caggcgcatg tcgatgtaat
    15661 ccataaacgg tttcagctta accattttgt ggcgggtctt tctggctggc ggttcagggt
    15721 atttgaggta gcgtctgacc gtccgttcag agcaaccaat ctgagtcgca atatcgacga
    15781 tgtacgcgcc ctgctggcgc atttgcttta tcatgtaaaa gtcctctctg ctcagcatgt
    15841 tgatgtcctt tctggtgtga gaaccttaag gaaacaacat gttgggtgga gcggacaatc
    15901 caaatggcga attaccgtct tatatcactg gcgctgacac ttaaggggga gcaggagaag
    15961 gcaaaagtat tcaacaaatg aaagtgaact ggatattcat tcacatgatt agcaataaac
    16021 gttgacaaaa tgtggcgtgg atcactataa tgcctgcaga ttttacgtcc cgtctcggta
    16081 caccaaatcc cagcagtatt tgcatttttt acccaaaacg agtagaattt gccacgtttc
    16141 aggcgcgggg tggagcagcc tggtagctcg tcgggctcat aacccgaagg tcgtcggttc
    16201 aaatccggcc cccgcaacca ctttccctta gagtcctttt tcaaatatac tgtgaagact
    16261 tcggccttcg tagtgggatt tgaaaaaatc cttctggaaa gtgctccaga ccgcagttgc
    16321 ggttataggg ttcagttata taaagccccg atttatcggg gttttttgtt atctgactac
    16381 agaataactg ggctttaggc ccttttttta tgtcttgggg gtgggcttgt ccacattaga
    16441 gcaaaaatta acagagatga ttactgcgcc agttgaggcc ctgggttttg aactggttgg
    16501 catcgaattt attcgcggtc gcacatccac actgcgcatc tatattgata gtgaagatgg
    16561 catcaatgtt gatgattgtg ctgatgtgag ccaccaggta agtgctgtgc tggatgttga
    16621 agatcccatc accgttgctt ataacctgga agtctcctca ccgggtctcg atcgcccact
    16681 gttcacggct gaacactacg cccgttttgt cggagaagag gtgactctgg ttctccgtat
    16741 ggcggtacaa aaccgtcgta aatggcaggg cgttatcaaa gcggtagacg gtgaaatgat
    16801 cacagttacc gtcgaaggta aagatgaagt gttcgcgctg agtaatatcc agaaggcgaa
    16861 cctggttccc cacttttaat agtctggatg aggtgaaaag cccgcgatga acaaagaaat
    16921 tttggctgta gttgaagccg tatccaatga aaaggcgcta cctcgcgaga agattttcga
    16981 agcattggaa agcgcgctgg cgacagcaac aaagaaaaaa tatgaacaag agatcgacgt
    17041 ccgcgtacag atcgatcgca aaagcggtga ttttgacacc ttccgtcgct ggttagttgt
    17101 tgatgaagtc acccagccga ccaaggaaat cacccttgaa gccgcacgtt atgaagatga
    17161 aagcctgaac ctgggcgatt acgttgaaga tcagattgag tctgttacct ttgaccgtat
    17221 cactacccag acggcaaaac aggttatcgt gcagaaagtg cgtgaagccg aacgtgcgat
    17281 ggtggttgat cagttccgtg aacacgaagg tgaaatcatc accggcgtgg tgaaaaaagt
    17341 aaaccgcgac aacatctctc tggatctggg caacaacgct gaagccgtga tcctgcgtga
    17401 agatatgctg ccgcgtgaaa acttccgccc tggcgaccgc gttcgtggcg tgctttattc
    17461 cgttcgcccg gaagcgcgtg gcgcgcaact gttcgtcact cgttccaagc cggaaatgct
    17521 gatcgaactg ttccgtattg aagtgccaga aatcggcgaa gaagtgattg aaattaaagc
    17581 agcggctcgc gatccgggtt ctcgtgcgaa aatcgcggtg aaaaccaacg ataaacgtat
    17641 cgatccggta ggtgcttgcg taggtatgcg tggcgcgcgt gttcaggcgg tgtctactga
    17701 actgggtggc gagcgtatcg atatcgtcct gtgggatgat aacccggcgc agttcgtgat
    17761 taacgcaatg gcaccggcag acgttgcttc tatcgtggtg gatgaagata aacacaccat
    17821 ggatatcgcc gttgaagccg gtaacctggc gcaggcgatt ggccgtaacg gtcagaacgt
    17881 gcgtctggct tcgcagctga gcggttggga actcaacgtg atgaccgttg acgacctgca
    17941 ggctaagcat caggcggaag cgcacgcagc gatcgacacc ttcaccaaat atctcgacat
    18001 cgacgaagac ttcgcgactg ttctggtaga agaaggcttc tcgacgctgg aagaactggc
    18061 ctatgtgccg atgaaagagc tgttggaaat cgaaggcctt gatgagccga ccgttgaagc
    18121 actgcgcgag cgtgctaaaa atgcactggc caccattgca caggcccagg aagaaagcct
    18181 cggtgataac aaaccggctg acgatctgct gaaccttgaa ggggtagatc gtgatttggc
    18241 attcaaactg gccgcccgtg gcgtttgtac gctggaagat ctcgccgaac agggcattga
    18301 tgatctggct gatatcgaag ggttgaccga cgaaaaggcc ggagcactga ttatggctgc
    18361 ccgtaatatt tgctggttcg gtgacgaagc gtaataaact gtagcaggaa ggaacagcat
    18421 gacagatgta acgattaaaa cgctggccgc agagcgacag acctccgtgg aacgcctggt
    18481 acagcaattt gctgatgcag gtatccggaa gtctgctgac gactctgtgt ctgcacaaga
    18541 gaaacagact ttgattgacc acctgaatca gaaaaattca ggcccggaca aattgacgct
    18601 gcaacgtaaa acacgcagca cccttaacat tcctggtacc ggtggaaaaa gcaaatcggt
    18661 acaaatcgaa gtccgcaaga aacgcacctt tgtgaaacgc gatccgcaag aggctgaacg
    18721 ccttgcagcg gaagagcaag cgcagcgtga agcggaagag caagcccgtc gtgaggcaga
    18781 agaatcggct aaacgcgagg cgcaacaaaa agctgaacgt gaggctgcag aacaagctaa
    18841 gcgtgaagct gctgaacaag cgaaacgtga agctgcggaa aaagacaaag tgagcaatca
    18901 acaagacgat atgactaaaa acgcccaggc tgaaaaagcc cgccgtgagc aggaagctgc
    18961 agagctcaag cgtaaagccg aagaagaagc gcatcgtaaa ctcgaagaag aagcacgtcg
    19021 cgttgctgaa gaagcacgtc gtatggcgga agaaaacaaa tggactgata acgcggaacc
    19081 gactgaagat tccagcgatt atcacgtcac tacttctcaa catgctcgcc aggcagaaga
    19141 cgaaagcgat cgtgaagtcg aaggcggtcg tggccgtggt cgtaacgcga aagcagcgcg
    19201 tccgaagaaa ggcaacaaac acgctgaatc aaaagctgat cgtgaagaag cacgcgcagc
    19261 agtacgtggc ggtaaaggcg gaaaacgtaa aggttcttcc ctgcagcaag gcttccagaa
    19321 gccagctcag gccgttaacc gtgacgttgt gatcggcgaa accatcaccg ttggcgaact
    19381 ggcgaacaag atggcggtta aaggctctca ggtcatcaaa gcgatgatga aactgggcgc
    19441 aatggcaacc atcaaccagg ttatcgatca ggaaaccgca cagctggttg ctgaagagat
    19501 gggccacaaa gttatcctgc gtcgtgaaaa cgagctggaa gaggcggtaa tgagcgaccg
    19561 tgacacgggt gctgcggctg aaccgcgcgc gccggttgtg accatcatgg gtcacgttga
    19621 ccacggtaaa acctctctgc tggactacat tcgttcaacg aaagttgcct ctggcgaagc
    19681 gggcggcatt acccagcaca ttggtgcata ccacgttgaa actgaaaacg gcatgatcac
    19741 cttcctggac accccggggc acgccgcgtt tacttcaatg cgtgcacgtg gtgcgcaggc
    19801 aacggacatc gtagtcctgg ttgttgctgc cgacgacggc gtgatgccgc agaccatcga
    19861 agcaatccag cacgcgaaag cggcgcaggt accggtggtg gttgcagtga acaagattga
    19921 taaaccagaa gccgatccgg atcgtgttaa gaacgaactc tcccagtatg gcatcctgcc
    19981 ggaagagtgg ggcggtgaaa gccagttcgt acacgtatct gcgaaagcgg gtaccggcat
    20041 cgatgaactg ctggacgcta tcctgctgca ggcggaagtt ctggagctga aagcggtacg
    20101 taaaggtatg gcgagcggtg cggttatcga atccttcctc gataaaggtc gtggtccggt
    20161 tgctaccgtt ctggtacgtg aaggtactct gcacaagggc gatatcgttc tgtgtggctt
    20221 cgaatacggt cgtgttcgtg cgatgcgtaa cgaactgggt caggaagtgc tggaagcggg
    20281 tccgtccatt ccggtggaaa tcctcggcct gtccggcgta ccggctgcgg gtgatgaagt
    20341 taccgttgta cgtgacgaga agaaagcgcg tgaagttgca ctgtatcgtc agggtaaatt
    20401 ccgcgaagtt aaactggcgc gtcagcagaa atctaaactc gagaacatgt tcgccaacat
    20461 gaccgaaggc gaagttcacg aagtgaacat cgtcctgaag gcagacgtac agggttctgt
    20521 ggaagcgatc tccgactcct tgctgaaact gtctactgac gaagttaaag tgaagatcat
    20581 cggttctggc gtaggtggta tcaccgaaac cgacgccacc ctggctgcgg cgtccaacgc
    20641 catcctggtt ggcttcaacg tacgtgctga tgcctctgca cgtaaagtga ttgaagcgga
    20701 aagcctggat ctgcgttact actccgtcat ctataacctg attgacgaag tgaaagcggc
    20761 gatgagcggt atgctgtctc cggaactgaa acagcagatt atcggtctgg cggaagttcg
    20821 tgacgtgttc aaatcgccga aatttggtgc catcgcaggc tgtatggtta ccgaaggtgt
    20881 ggttaaacgt cacaacccga tccgcgttct gcgtgacaac gtggttatct acgaaggcga
    20941 gctggagtcc ctgcgccgct tcaaagatga cgttaacgaa gtccgtaacg gtatggaatg
    21001 tggtatcggc gttaagaact acaacgacgt ccgcactggc gatgtgatcg aagtattcga
    21061 aatcatcgag atccaacgta ccatcgctta aggtttttag ggttccatgc gggataaggc
    21121 gtttacgccg catccggcat ggattgcctg atgtgacgct ggcgcgtctt atcaggccaa
    21181 cgtgaacagc ccttccacct gaaattaatt ttaaaaaggg gctaacagcc ccttttttgt
    21241 caggagaatt tattatggcg aaagaatttg gtcgcccgca gcgcgtagcg caggaaatgc
    21301 aaaaagagat cgctctcatc ctgcagcgtg aaattaaaga tcctcgcctg ggcatgatga
    21361 ccaccgtttc cggtgtcgaa atgtctcgcg acctggcgta tgccaaagta tatgtgacgt
    21421 tcctcaacga caaagatgaa gacgcggtta aagcgggcat caaagcgttg caagaagctt
    21481 ctggtttcat ccgcagcctg ctggggaaag cgatgcgcct gcgtatcgtg ccggaactga
    21541 ccttcttcta cgacaactct ctggttgaag ggatgcgcat gtcaaacctg gtgaccagcg
    21601 tggtcaaaca tgacgaagaa cgtcgtgtta acccggacga cagcaaggag gactaatgag
    21661 tcgtcctcgt cgtcgcggtc gcgacattaa cggcgttttg ttgctggata aaccgcaggg
    21721 tatgtccagc aatgatgcgc tgcaaaaagt gaaacggatt tataacgcca accgtgccgg
    21781 gcataccggt gcgctggacc cgctggcaac aggcatgttg ccaatttgcc tcggggaagc
    21841 gacgaagttt tcccagtatc tgctggactc cgacaaacgc tatcgggtca ttgcgcgtct
    21901 tggacagcgt accgatactt ctgatgccga cggacaaatc gttgaagaac gtccggtaac
    21961 ctttagcgca gagcaactgg cggcggcgct ggatactttc cgtggcgata tcgaacagat
    22021 cccttcgatg tattcagcac tgaaatatca gggtaaaaaa ctgtacgaat atgcgcgtca
    22081 gggcattgaa gtaccgcgtg aagcgcgccc gattaccgtt tatgaattgc tgtttattcg
    22141 ccacgaaggc aatgagctgg agctggaaat tcactgctca aaaggcactt atatccgcac
    22201 catcattgat gacctgggtg aaaaactcgg ttgtggcgcg catgttattt acctgcgtcg
    22261 tctggcggta agtaaatatc cggttgaacg gatggtgacc ctggaacatc tgcgtgaact
    22321 ggtcgaacaa gccgaacagc aggatattcc ggctgcggag ttacttgatc cattactgat
    22381 gccaatggac agtccagctt cggactaccc ggtggtgaat cttccgttaa cgtcttctgt
    22441 ttacttcaaa aatggtaacc cagttcgtac atccggtgcg ccgctggaag gactggttcg
    22501 cgttacagaa ggtgagaacg gcaagtttat cggtatgggc gaaattgacg atgaaggccg
    22561 cgtcgcgcct cgtcgcctgg tggttgaata cccggcgtaa tgttaaccgt cttgcgataa
    22621 caggtcgcta cgagtagaat actgccgctt aacgtcgcgt aaattgttta acactttgcg
    22681 taacgtacac tgggatcgct gaattagaga tcggcgtcct ttcattctat atactttgga
    22741 gttttaaaat gtctctaagt actgaagcaa cagctaaaat cgtttctgag tttggtcgtg
    22801 acgcaaacga caccggttct accgaagttc aggtagcact gctgactgca cagatcaacc
    22861 acctgcaggg ccactttgca gagcacaaaa aagatcacca cagccgtcgt ggtctgctgc
    22921 gcatggtttc tcagcgtcgt aaactgctcg actacctgaa acgtaaagac gtagcacgtt
    22981 acacccagct catcgagcgc ctgggtctgc gtcgctaatt cttgcgagtt tcagaaaagg
    23041 gggcctgagt ggcccctttt ttcaagctga cggcagcaat tcactggaaa ctaatgtatt
    23101 gttgctatga atgatcttcc gttgcagagg ttcgcgcggc taatgagagg ctttacccac
    23161 atagagctgg gttagggttg tcattagtcg cgaggatgcg cagaagatcg ggtattaaca
    23221 ccagtgccgt aaggtactgt ctaagaaaga gaaaggatat tacattgctt aatccgatcg
    23281 ttcgtaaatt ccagtacggc caacacaccg tgactctgga aaccggcatg atggctcgtc
    23341 aggctactgc cgctgttatg gttagcatgg atgacaccgc ggtattcgtt accgttgttg
    23401 gacagaaaaa agccaaacca ggtcaggact tcttcccact gaccgttaac tatcaggagc
    23461 gtacctacgc tgctggtcgt atcccgggta gcttcttccg tcgtgaaggt cgtccaagcg
    23521 aaggcgaaac cctgatcgcg cgtctgattg accgcccgat tcgcccgctg ttcccggaag
    23581 gcttcgtcaa cgaagttcag gttatcgcca ccgtggtttc tgttaacccg caggttaacc
    23641 cggatatcgt cgcgatgatt ggtgcttccg cagcactgtc tctgtctggt attccgttca
    23701 atggcccgat tggtgctgcc cgcgtaggtt acatcaatga ccagtacgta ctgaacccga
    23761 ctcaggacga gctgaaagag agcaaactgg atctggttgt tgccggtact gaagccgctg
    23821 tactgatggt tgaatctgaa gctgaactgc tgagcgaaga tcagatgctg ggcgcagtag
    23881 tgttcggtca tgaacaacag caggttgtta ttcagaacat caatgaacta gtgaaagaag
    23941 ccggtaaacc gcgttgggac tggcagccgg agccggtaaa cgaagcgctg aacgcgcgcg
    24001 ttgctgcact ggctgaagct cgtctgagtg atgcttaccg catcaccgac aaacaagagc
    24061 gttatgcgca ggttgatgtc atcaaatctg aaaccatcgc gacgctgctt gctgaagacg
    24121 aaaccctgga cgaaaacgaa ctgggtgaaa ttctgcacgc tatcgagaaa aacgttgttc
    24181 gtagccgcgt actggcaggc gaaccgcgta tcgacggtcg tgaaaaagat atgatccgtg
    24241 gtctggatgt gcgtactggc gtgctgccgc gtactcacgg ttctgcgctg ttcacccgcg
    24301 gtgaaacgca ggcactggtt actgcaacgc tgggtaccgc tcgtgacgcg caggttcttg
    24361 atgaactgat gggcgaacgt accgatacct tcctgttcca ctacaacttc cctccgtact
    24421 ccgtaggcga aaccggcatg gtcggttctc cgaagcgtcg tgaaattggt cacggtcgtc
    24481 tggcgaagcg cggcgtgctg gcagtcatgc cggatatgga caaattcccg tacaccgtac
    24541 gtgtagtgtc tgaaatcacc gaatccaacg gttcttcttc tatggcttcc gtgtgcggcg
    24601 cgtctctggc gctgatggac gcaggtgtgc caatcaaagc tgccgttgcg ggtatcgcaa
    24661 tgggtctggt gaaagaaggc gacaactacg ttgtactgtc tgacattttg ggcgacgaag
    24721 atcacctggg cgatatggac ttcaaagttg caggttcccg cgacggtatc tctgcactgc
    24781 agatggatat caaaattgaa ggtatcacca aagagatcat gcaggttgcg ctgaaccagg
    24841 ctaaaggtgc gcgtctgcat atcctgggcg taatggaaca ggcgatcaac gcgccgcgtg
    24901 gcgatatctc tgagttcgca ccgcgtatcc ataccatcaa gatcaacccg gataagatca
    24961 aagacgttat cggtaaaggc ggctctgtta tccgtgccct gaccgaagaa actggcacta
    25021 ccatcgaaat cgaagatgac ggtactgtga agatcgcagc gaccgacggc gagaaagcga
    25081 aacatgctat tcgtcgtatc gaagagatca ctgcagaaat cgaagtgggc cgcgtctaca
    25141 ctggtaaagt gacccgtatc gttgactttg gcgcatttgt tgccatcggc ggcggtaaag
    25201 aaggtctggt ccacatctct caaatcgctg acaaacgcgt tgagaaagtg accgattacc
    25261 tgcagatggg tcaggaagta ccggtgaaag ttctggaagt tgatcgccag ggccgtatcc
    25321 gtctgagcat taaagaagcg actgagcagt ctcaacctgc tgcagcaccg gaagctccgg
    25381 ctgctgaaca gggcgagtaa ggttgccatt tgccctccgc tgcggcgggg ggcttttaac
    25441 cgggcaggac gccttgttag caaccgggaa caggacgttc attcaaccgt ggtcttcggg
    25501 agtgggaaat gaagcctttt ttgcgctggt gtttcgttgc gacagcactt acgcttgcag
    25561 gatgcagtaa tacttcctgg cgtaaaagtg aagtcctcgc ggtaccattg caaccgactt
    25621 tacagcagga agtgattctg gcacgtatgg aacaaatcct tgccagtcgg gctttaaccg
    25681 atgacgaacg cgcacagctt ttatatgagc gcggagtgtt gtatgatagt ctcggtctga
    25741 gggcattagc gcgtaacgat ttttcgcaag cgctggcaat ccgaccggat atgcctgaag
    25801 tattcaatta cttaggcata tatttaacgc aggcaggcaa ttttgatgct gcctatgaag
    25861 cgtttgattc tgtacttgag cttgatccaa cttacaacta cgcgcacttg aatcgcggga
    25921 tcgcattata ttacggcggt cgtgacaagt tagcgcaaga tgatctgctg gcgttttatc
    25981 aagacgatcc caatgatcct ttccgtagtc tgtggcttta tctcgccgag cagaagctcg
    26041 atgagaagca ggctaaagaa gtgttgaaac agcacttcga aaaatcggat aaggaacagt
    26101 ggggatggaa cattgtcgag ttctacctgg gcaacattag cgaacaaacg ttaatggaaa
    26161 ggctcaaggc ggacgcaacg gataacacct cgctcgctga gcatctcagt gaaaccaact
    26221 tctatttagg taagtactac ctaagtctgg gggatttgga cagcgccacg gcactgttca
    26281 aactggcggt tgccaacaac gttcataact ttgttgagca ccgatacgca ttgttggaat
    26341 tatcgctcct gggccaggac caagatgacc tggcagaatc ggaccagcaa tagctgacgt
    26401 acacatcagc ccgtaatctt ttttgattgc catcacctta acgggtgagg gcgttgttgt
    26461 tcgttaatac acctactttg agccggttca cacttttcaa tgaaaattgc tgatcaattt
    26521 catgatgagt tatgtagact ggccgccatt aattttgagg cacacgtact acatggctga
    26581 attcgaaacc acttttgcag atctgggcct gaaggctcct atccttgaag cccttaacga
    26641 tctgggttac gaaaaaccat ctccaattca ggcagagtgt attccacatc tgctgaatgg
    26701 ccgcgacgtt ctgggtatgg cccagacggg gagcggaaaa actgcagcat tctctttacc
    26761 tctgttgcag aatcttgatc ctgagctgaa agcaccacag attctggtgc tggcaccgac
    26821 ccgcgaactg gcggtacagg ttgctgaagc aatgacggat ttctctaaac acatgcgcgg
    26881 cgtaaatgtg gttgctctgt acggcggcca gcgttatgac gtgcaattac gcgccctgcg
    26941 tcaggggccg cagatcgttg tcggtactcc gggccgtctg ctggaccacc tgaaacgtgg
    27001 cactctggac ctctctaaac tgagcggtct ggttctggat gaagctgacg aaatgctgcg
    27061 catgggcttc atcgaagacg ttgaaaccat tatggcgcag atcccggaag gtcatcagac
    27121 cgctctgttc tctgcaacca tgccggaagc gattcgtcgc attacccgcc gctttatgaa
    27181 agagccgcag gaagtgcgca ttcagtccag cgtgactacc cgtcctgaca tcagccagag
    27241 ctactggact gtctggggta tgcgcaaaaa cgaagcactg gtacgtttcc tggaagcgga
    27301 agattttgat gcggcgatta tcttcgttcg taccaaaaac gcgactctgg aagtggctga
    27361 agctcttgag cgtaacggct acaacagcgc cgcgctgaac ggtgacatga accaggcgct
    27421 gcgtgaacag acactggaac gcctgaaaga tggtcgtctg gacatcctga ttgcgaccga
    27481 cgttgcagcc cgtggcctgg acgttgagcg tatcagcctg gtagttaact acgatatccc
    27541 gatggattct gagtcttacg ttcaccgtat cggtcgtacc ggtcgtgcgg gtcgtgctgg
    27601 ccgcgcgctg ctgttcgttg agaaccgcga gcgtcgtctg ctgcgcaaca ttgaacgtac
    27661 tatgaagctg actattccgg aagtagaact gccgaacgca gaactgctgg gcaaacgccg
    27721 tctggaaaaa ttcgccgcta aagtacagca gcagctggaa agcagcgatc tggatcaata
    27781 ccgcgcactg ctgagcaaaa ttcagccgac tgcagaaggt gaagagctgg atctcgaaac
    27841 tctggctgcg gcactgctga aaatggcaca gggtgaacgt actctgatcg taccgccaga
    27901 tgcgccgatg cgtccgaaac gtgaattccg tgaccgtgat gaccgtggtc cgcgcgatcg
    27961 taacgaccgt ggcccgcgtg gtgaccgtga agatcgtccg cgtcgtgaac gtcgtgatgt
    28021 tggcgatatg cagctgtacc gcattgaagt gggccgcgat gatggtgttg aagttcgtca
    28081 tatcgttggt gcgattgcta acgaaggcga catcagtagc cgttacattg gtaacatcaa
    28141 gctgtttgct tctcactcca ccatcgaact gccgaaaggt atgccgggtg aagtgctgca
    28201 acactttacg cgcactcgca ttctcaacaa gccgatgaac atgcagttac tgggcgatgc
    28261 acagccgcat actggcggtg agcgtcgtgg cggtggtcgt ggtttcggtg gcgaacgtcg
    28321 tgaaggcggt cgtaacttca gcggtgaacg ccgtgaaggt ggccgtggtg atggtcgtcg
    28381 ttttagcggc gaacgtcgtg aaggccgcgc tccgcgtcgt gatgactcta ccggtcgtcg
    28441 tcgtttcggt ggtgatgcgt aatcatcgct gaacagcgaa cacaatctgt aaaataatat
    28501 atacagcccc gatttttacc atcggggctt tttttctgtc ttttgtactc gtgtactggt
    28561 acagtgcaat gcataacaac gcagtcgcac tatttttcac tggagagaag ccctcatggc
    28621 aacactaacc accacccaaa cgtcaccgtc gctgcttggc ggcgtggtga ttatcggcgg
    28681 caccattatt ggcgcaggga tgttttctct gccagtggtc atgtccgggg cgtggttttt
    28741 ctggtcaatg gcggcgctga tctttacctg gttctgtatg ctgcattccg gcttgatgat
    28801 tctggaagct aacctgaatt accgaatcgg ttcgagtttt gacaccatca ccaaagattt
    28861 gctgggcaaa ggctggaacg tggtcaacgg tatttccatt gcctttgtgc tctatatcct
    28921 gacctatgcc tatatttctg ccagtggttc aattctgcat cacaccttcg cagagatgtc
    28981 gctaaacgtc ccggcacggg cggcgggttt tggttttgca ctgctggtag cgtttgtggt
    29041 gtggttgagt actaaggccg tcagccgcat gacggcgatt gtactggggg cgaaagtcat
    29101 taccttcttc ctcaccttcg gtagcctact ggggcatgtg cagcctgcga cattgttcaa
    29161 cgtcgccgaa agcaatgcat cttatgcgcc gtatttgctg atgaccctgc cgttctgtct
    29221 ggcatcgttt ggttatcacg gtaacgtgcc gagcctgatg aagtattacg gcaaagatcc
    29281 gaaaaccatc gtgaaatgcc tggtgtacgg tacgctgatg gcactggcgc tgtataccat
    29341 ctggttgctg gcgacgatgg gtaacatccc gcgtccggag tttatcggta ttgcagagaa
    29401 gggcggtaat attgatgtgc tggtacaggc attaagcggc gtgctgaaca gccgtagcct
    29461 ggaccttctg ctggtcgtgt tctcaaactt tgcggtagcg agttcgttcc ttggcgtaac
    29521 gctgggtttg tttgactatc tggcagatct gtttggtttc gatgactcag ccctggggcg
    29581 cttgaaaacg gcattgctga cctttgcccc gcctgttgtg ggtggtctac tgttcccgaa
    29641 cggattcctg tacgccattg gttatgctgg tttagcagct accatctggg cggcaattgt
    29701 tccggcgctg ttagcccgcg catcgcgtaa acgctttggc agcccgaaat tccgtgtctg
    29761 gggcggcaag ccgatgatta cgctgattct ggtgtttggc gttggcaacg cactggtcca
    29821 tatcttatcg agctttaatt tgctgccggt gtatcagtaa tcagcggtgc cttatccgac
    29881 atttctgctg cctacacaat gcctgatgcg cttcgcttat caggtctacg taggacagcg
    29941 ttgccagctc ggataaggct tcccgcgtta agacacacta tcccaacaac tcttccttca
    30001 catccatcgc cagttcaaac gaatgcaggc gcgcctggtg gtcgaaaatc tgcccgttaa
    30061 ccataatttc atcggcgtcg gtttcgcgca ggatcgactg caagccgtga cgcactttcg
    30121 ctttatcacc caccaacgac atactcagcg cctgctgcac gccatactgc tcagacggtg
    30181 accagaactg atccatattt tgaatcggcg gcggcagttg tccggtttcg ccacggcgca
    30241 gcttcacaaa ggcttgctgc attgaggtaa atagaaattc agcgtcgcgg ttgctgtcgg
    30301 cggcgataat attgatgcac accatcgcgt atggtttttc cagtcgtgcc gacggtttga
    30361 agttgctgcg ataaagatgc agcgcctgga acaacatatc cggcgcgaag tgtgaggcaa
    30421 acgcgaacgg cagaccaagc tgcgccgcca gttgcgcgct gtaaaggctg gagcctaaca
    30481 accacacggg gattttctcg ccatagcctg gtaccgggcg cacatgcgga ttgggatcgc
    30541 gggcgtcaaa ccagtccacc agctccgcga catcgcgggg gaaattatca atatcgccgc
    30601 tcatatggcg acgcagcgcc atcattgtcc gttggtcact acctggcgca cgacccagcc
    30661 ccaaatcgat tcgccccgga tagagtgtat tgagcgtgcc gaactgttcg gcaatgacca
    30721 acggtgagtg gttaggcaac atcacgccgc cagaccccag atgcagcgtg gtggtattcg
    30781 ccgccagata gccaatcaat accgacgtgg cagcactggc aatgccagtc atattgtggt
    30841 gttctgccag ccagtagcga tgatagccgc gcttttcagc cagacgggcg agatcgagag
    30901 agtgggagaa tgcttctcgc gctgaagaac cttcgggaat gggggccaga tcgagtagcg
    30961 aaaacgcaat ggttttatca gtcataacag ctcactttgt taccgattaa tctttaatac
    31021 tgcattaaag agtagttaaa gttgttaaca aagtgagctg tgctctaaat aattcgagtt
    31081 gcaggaaggc gacaagagag tgaatcccca ggagcttaca taagtaagtg actggggtga
    31141 acgaatgcag atgcagcaca tgcaacttga agtatgacga gcatatttac ttaggcttgc
    31201 agctccagcc cagccagccg ccgccagtag ccgttacagt cgctgtttgc cgtcaacggc
    31261 agtggcgcag cgccattttc attagcgcgg aaggcgtcga gcatcgcgaa agtgtcagta
    31321 ccctgcggtg acaggcggac cacatcaacc aatccctgca tggatgtcag ctcgttaccg
    31381 aggttgtaaa cgtagccgct catggtctga atgccattga gtacaaacac ttgttggttt
    31441 tcctgcgaca gcacgttgcg cccgttcgga tacttaatgc agcaggtttc acactcatct
    31501 ttcgggcggt cttccgaacg cgcggtaaag cagcgggcgg agtaggccag cggtagatga
    31561 ccgtagctca ggacttccac ttcaaactgg ttgcgaatcc ccaactcatc gcactgatta
    31621 agcagattca ccagccagtc gcgggaaagc tccaccggca tacaccagcg catcatgccc
    31681 tgtttgagca atattttcag cgtcaccgcg ttgtagcagt tcagcgcgtg cccggcgacg
    31741 aacggcagtt tgcgttcagc gcacatattc accacgccga gatcgctggc ttcaatcaga
    31801 aactcaccgt tctcaacata gcgtttcagt tcgcccagtt cagaggatgc ctgcaccagc
    31861 gccagcgtgg agagcactat ctgcttcccg cttccggcga gcgattttgc catctccagc
    31921 cagtcgccaa ctttggttgc ccggcgcttg ctgcataccg cttcaccaag ataaatcacg
    31981 tcggcgctgc tggtggcggc ctgctgataa aattcttcca gcgcctcttt tggccagtac
    32041 cacagcactg gccctaagga atatttcatt gctcttctca ctgccattta cggtgatacg
    32101 cgccaagggt ggtctgcgtg ccttcggaca tcgacccgag cgtctccatc cacgcgcttt
    32161 gcggtacgaa gttttgcgga tcggccttac aacggtcgat agcctgacgc cagactttcg
    32221 ccacctggct gacatacgcc gggctacgct ggcggccttc aattttcacc gaagcaatat
    32281 tcgccgccat taactccggc agcagttcca gggtattgag actggttggt tcttccagcg
    32341 cgtgatagcg ctcgccgtcc accagataac gccctttaca cagcgtcgga taacctgcgt
    32401 tttcgccatc ctgataacgg tcgatcagca cttcgttcag gcgggattcc agcccctgcg
    32461 gcgtttgttg ccagcgcacg aaacgggccg gagaacacgc gcccacggtg ttgggcgact
    32521 cacccgtcag atacgacgac agatagcaac gaccttccga cataatgcac aggctgccaa
    32581 aagcaaagac ttccagcggg acaggtgtga cccgtgccag ctgtttcacc tgatgaatcg
    32641 acaacacgcg cggcagcacc acgcgggcaa catcaaaatg gcgatgataa aagttaatcg
    32701 cctcttcatt ggtcgccgaa gcctgcactg acacatgacg ctcaatatgc gggtaacgct
    32761 cggcggcgta ctccagcatg gcgaggtcgg cgaggatcag cgcgtcggca cccagctgcg
    32821 ccgccatatc cacggcgcgc tgccaacggg cgtaaccgtc cggatgcgca aaagtgttaa
    32881 tcgcgatgtg aagtttgcgg cgatgttgat ggacaaaact caccgcttcc tgcaattttt
    32941 tctcggtaaa gttaaggccg gcgaagtgac gggcattagt atcatctttt agcccgatat
    33001 aaacagcatc tgcgccgttt tcgatggccg ccttaagcgc cgggagattt ccggcagggc
    33061 agagcagctc cataattttt cctgaaggtt gcgctttcaa aacgcaaaaa tgttaacgaa
    33121 ctgggatttt agttaacctt gctgcgacaa tttttgattt aaggcagtta aagttgtatt
    33181 ggtggtagca gcaatttcat atggaattgt tgatttatac cgctatgtta tttttctgat
    33241 atggcaaaat aggatgattg ttgaaacagg gagtaaaact cgtgttggat aaactgcgtt
    33301 cccgtattgt gcatttgggg ccatctctgt tgagtgtacc ggtaaaactg acgccatttg
    33361 cgctaaaacg ccaggttctt gagcaggtct taagctggca attccgtcag gcgctggatg
    33421 atggtgagct ggagtttctt gaaggccgct ggttaagtat tcatgtgcgt gatattgacc
    33481 tgcaatggtt tacctcggtg gtgaatggca aactggtggt cagccagaac gcgcaagctg
    33541 atgtgagttt tagtgccgac gccagcgatc tgctgatgat tgcggcgcgt aaacaagatc
    33601 cggatacgct cttcttccag cgtcggctgg tgattgaagg cgatacggag ctggggctgt
    33661 atgtgaaaaa cctgatggac gccattgaac tggagcaaat gccgaaagcc ttacgcatga
    33721 tgctgctgca actggcggat tttgttgagg cgggaatgaa aaccgcgcct gaaaccaaac
    33781 agacatcggt aggtgaacca tgctaattcg agtagaaatt cccattgatg cgccgggtat
    33841 tgatgccctg ctgcgtcgct cattcgaaag tgatgcggaa gcgaagctgg ttcacgatct
    33901 gcgtgaagat ggctttctga cgctggggct ggtggcgaca gatgacgaag gtcaggtcat
    33961 tggctatgtg gcatttagtc cggttgatgt gcagggcgaa gacctgcaat gggtcggcat
    34021 ggcaccgttg gcggttgatg aaaaataccg tggacagggg ctggcacgcc aactggtcta
    34081 tgaaggactc gattcgctta atgagttcgg ctatgccgca gtggtgacgc tgggcgatcc
    34141 ggcgctgtac agtcgtttcg gctttgaact ggcggcgcat cacgatctgc gttgccgctg
    34201 gccgggcact gaaagcgcct tccaggtaca tcgtttagcg gatgacgcgc tgaatggcgt
    34261 aaccggtctg gttgagtatc acgagcattt caatcgcttt taatctccgg ggtttgcaga
    34321 ctgcttaaca gctctgcaaa ccctgcgcct tccgctacca gacgctcttt ttgccgtttg
    34381 gtcagttgct taacgcgata ttccgcccgt aacgccagcg aacgatcgcc cactggcgca
    34441 gaaaacgcca gcgtcagttc tcctttcccg cgcagtgctt tcgccccttt gccgctttgg
    34501 tgctgctgat agcggcgttc gacatccgtg gtgatcccgg tataaagctt attgtcggcg
    34561 gtacggatca agtaaagaaa ccagggtgtc atcgtctgcg tcgcatgtta aggtcaggcc
    34621 cacagtataa aagagagcag caatggaaac actcatcacc atcagccgtt ggttggcaaa
    34681 acaacatgtt gtcacctggt gtgtgcagca ggaaggggaa ctgtggtgcg ctaatgcttt
    34741 ttatctgttt gatgcgcaga aagtcgcctt ctacattttg acggaagaaa aaacgcgcca
    34801 cgcgcagatg agtgggccgc aggctgcggt tgccggaacg gtaaacggtc agccgaaaac
    34861 ggtagcgtta attcgcggcg tgcagtttaa aggtgagatc cgcaggctgg aaggtgagga
    34921 aagcgacctc gcgcgccagg cgtacaatcg tcgctttccg gttgccagaa tgctgtcggc
    34981 cccggtgtgg gaaatccggc tcgatgaaat caaattcacc gacaacacgc tgggctttgg
    35041 taaaaaaatg atttggctac gcgactcagg caccgagcag gcgtaacgcc tcgcggttaa
    35101 acgctggcag atcgtccggt gtccggctgg taaccagctg atctttatca accacgactt
    35161 cctgatcgta aaattccgcg cccgcatttt taacatcaat aatgatcggt ttaactgcgg
    35221 tcagtttgcg cccccgaatc acatcggcgc tgatcagcag ctgcggaccg tgacagatgg
    35281 cgaaaacagg tttgccgcta ttcacaaaat cacgggtaaa ggtgacaaaa cggttgtcgc
    35341 cacgcagata atccggtgaa tgaccacccg gtagcagcag ggcatcaaac tccgctggcg
    35401 tcacttcatc gatggattta tcgatggtca cgctggcttc tccttttttg cctttcaccg
    35461 ttttacccgc ttgtttttca atggtaatca cttcgtgtcc ggctttacgg aactcgtctg
    35521 cgggtgaagt aaattctgaa tcctcaaatt catcactgat taaaacggca attttcttac
    35581 tcatgcttcc tccgcgttgt tgcattggat gaggcgaatt tcacacaatc ggggataatt
    35641 acctcatggt ctattaagcc tggtgcatga gccgtggagc gcaaggcaga caattctgta
    35701 aaggagcgaa gatgagtcag gtactgatta caggcgcaac ggggctggtg ggcggtcacc
    35761 tgctgcggat gttgattaac gaaccgaaag ttaacgccat agccgcgccg acgcgacgtc
    35821 cgttgggcga tatgcccggg gtgtttaatc cccatgatcc gcaactgagt gacgcgttgg
    35881 cgcaggtcac cgaacccatc gacattgtgt tttgttgtct cggcaccacg cggcgagaag
    35941 cggggagcaa agaggcgttt attcatgccg attacacgct ggtagtggat accgcattaa
    36001 ccgggcggcg actgggcgcg cagcatatgc tggtggtcag tgctatgggt gccaatgccc
    36061 actcgccgtt tttctataac cgcgtcaaag gggagatgga agaagcatta attgcccaga
    36121 actggccgaa attgaccatt gctcgcccgt cgatgttgct gggcgatcgt agcaaacagc
    36181 ggatgaacga aacgcttttt gcgccgctgt ttcgtttact gcccggtaac tggaaatcca
    36241 ttgatgcgag agacgtggcg cgggtcatgc tggcagaggc gatgcggcct gagcatgaag
    36301 gcgtgactat tttaagctct tcagaattac gcaaaagagc tgaataatta ttcccaagaa
    36361 ttgatagtga gcgtaatatt cacgcgctca attatcataa cttattcctg gggaatgcta
    36421 tgactggtca gtcttcatct caggcggcaa cgcccattca gtggtggaaa cccgcgcttt
    36481 tctttctcgt tgtcattgcc ggtctctggt atgtgaaatg gcaaccttac tacggcaaag
    36541 cgtttactgc tgccgaaacc cacagtatcg gtaaatctat ccttgcgcag gcggatgcta
    36601 atccgtggca ggcggcgttg gattacgcga tgatctattt cctcgcggta tggaaagcgg
    36661 cggtgctggg ggtgatcctc ggttcgttga ttcaggtgct gatcccgcgt gactggttgt
    36721 tgcgtacgct tggacaatcg cgctttcgcg gcacgctgct gggaacgctg ttttcgctgc
    36781 cgggcatgat gtgtacctgc tgtgcggctc cggtcgcggc gggaatgcgt cgccaacagg
    36841 tatcgatggg cggtgcgctg gcattctgga tgggcaatcc ggtgttaaac ccggcgacgc
    36901 tggtgtttat ggggtttgtc ctcggctggg gttttgcggc gattcgtctg gtggccgggc
    36961 tggtgatggt gttgctgatt gcgacgctgg tgcaaaaatg ggtgcgtgaa acaccgcaaa
    37021 cgcaggcacc ggtcgaaatt gacataccgg aagcacagag cggttttttt agccgctggg
    37081 gcagggcgct atggacactt ttctggagta cgatcccggt ttacatcctt gcagtactgg
    37141 tgttgggtgc cgcacgcgtc tggttattcc cccatgccga tggtgctgtc gataacagcc
    37201 tgatgtgggt ggtggcgatg gcggtagcag gatgcttgtt tgtcattccc acggcagcag
    37261 aaattccgat tgtacaaacg atgatgctgg caggtatggg aaccgctccg gcgctggcat
    37321 tgttgatgac gctcccggca gtgagtttgc cgtcactgat tatgctgcgc aaagcgttcc
    37381 cggcgaaagc cttatggctg acgggggcga tggtggcagt gtcaggcgtg attgtcggcg
    37441 ggctggcgct gttattctga tttgagggaa aacatgtcgg atgcggcgta aacgccttat
    37501 ccgacctacg gtttgcatcg ttgtaggcct gataagacgc ggcaagcgtc gcatcaggca
    37561 ttacaagggg ctgctattta ataaacgtaa acgctgtggt tacccgcttt acgccgctca
    37621 cccggctggc aatatctgcc gccgctttcg cttcacgttc agtcaccagc cccatcagga
    37681 acacttcacc gttttcggtg gtcactttca cgttggacga tttcaccagg tcgctggtta
    37741 agagctgcga acgcactttg gtggtgatcc acgtatcgtt agatgcttcg cccagaccaa
    37801 tcggctggcc ctgacgaatc tcgttataca cttcgttggc accgtctacg cccatagcaa
    37861 tctgtttggc gcgagccgaa agttcagcgt ttggtgactg cccaaccagc agcactttgc
    37921 cctgatacgc cgtgacatta atacgggttt ctttcttaat ctgttcgtct ttcgacaatg
    37981 cgctgttcac gcgcacttcc agggtaccat cgtccacctg ggtgccgaca ctgcgtgggt
    38041 cagttgcggc tttggtaccc acagcagcgg tacccactac ggcagcggca acacaacctt
    38101 gcaacagcag cgcggaaata aggactgcga ttggcgataa tgccttcatg tattctcctt
    38161 aatcatcctg gtgagggaaa agcgtgttat cgatcagatc gcacaggcaa tttaccgtca
    38221 gcatatgcat ttcctgaatg cgagcactac gatgcgaagg aatgcggatc tccacatcct
    38281 gtggccctaa caaacctgca agctcgccgc cgtcatagcc ggtcaatgcc acaatggtca
    38341 tatcacgcgt aacggcggct tcaactgctt taacaatatc gcggctgttg ccacgggtgg
    38401 aaatggctaa caatacatct cccgcatgac ccagcgcccg cacctgtttt gcatacactt
    38461 catcatgtaa gcgatcgttg gcaatcgccg ttaagacaac attatcagta tttagtgcaa
    38521 tggcaggtaa gctgggccgc tccgtttcga aacggttgat catgctggca gcaaaatgct
    38581 gtgcattggc agcggaagtt ccattaccac aacagaggat tttgttgcca ttgagcagag
    38641 actgaaccag cgtcatggct gcacgggaga tggcatccgg aagcgcctct gccgccgcaa
    38701 tttgagtttg aatgctttca gtgaagcaag ctttaattct ttcttgcacg ctaatcctta
    38761 aaccttaatt atgagtggtc attaaaggca tccttaatcc actcaacctc attcccggtg
    38821 aaggctacca catcgaaccg gcaatccaca gtatcaaaac tcccattatg acgcgcgagc
    38881 cacaagcggg cagtctgtaa taatttgtgt tgtttgctgc gggtcacact ggctgccgcg
    38941 ccgccataaa gcgcagagcg gcgatagcgt acctcgacaa aaacggtggt ccggccttca
    39001 cgcattatca gatcgatctc gccgccacgc tcgttcacgt tagcggcgat aaaccgcagt
    39061 cctttgcctt ccagccagcg acgcgcttgt gcttcccacg catcgccggt ctgtttggtg
    39121 gttaactgac ggggactacc tgaccttgtt ggtactgtag ccatgataac ttcctgttaa
    39181 tcacgcaatc cgggtttgcc gtcaggcttc cggtattacc gttgatttca aaaccctgaa
    39241 cctggcgcat ttgtgagaaa tgatttgcca gcgaccaggc atcgacgccc atcgcataca
    39301 tgcgagccag tgaataatcg ttattcaccg cgctgagtgc ctgctgcatt aacggcagat
    39361 taccgcccgc cagcatcggg atttcgctgt actgcaaacc ttccatctcc agacggaaat
    39421 ccgggcctgc ggtgccttgt gcgctgcggg agctggcgta cagcattgca ccgctctggc
    39481 taccgttacg catggcgatc atcggtttga taaaagcgat ttcacccggc gttgccacaa
    39541 tgtacaccgc atcgacacga ccgccattat tggtgaactg gtcatcggtt ggcgtggttt
    39601 gcagcgttgg attgttggtc gtcatgccgg agtcggttgt cgctctggga gtaatcgggg
    39661 tacccgttaa agcgatacca gaaccgccgt taacacccgc gcgtaattcg ctggtggaac
    39721 caaatttttg ttgcagaacg gtgccgccgc ccagtttctg ccactcttgc gcaaacgcat
    39781 tggctacgcg atcgcccaat gaactgcgtg ggatcagcac cagcggcgct tgtttaccct
    39841 ggtcacgaat atgacgcgct gcatcgcgcg cttcgtcttc aggtgaaagc gcgaagtaac
    39901 aaatattgac gcggttttcg atattctctg gctggttcag tgccagcacg ttcagcgggg
    39961 tgttgctctt cagcaactct tcaacgttat ttttcagcaa cggaccaacc acaatactcg
    40021 cgccatcctg ctgaacctgg cttaagatct ggctaagtgg ttgtgatgag gtatcgtaga
    40081 ttttcagctc tgcggatgga tttgcgggtg cgcttaccgc tgcggtgctt gtcgccgggg
    40141 cgcttacagg caccggctgg gccgcaggct gatcaccggt cagatcgcta accgaggctt
    40201 gtgccgggct ggcaacgcca tccacggttt gcggctgagg ttgttcagct acgtctgcgg
    40261 caggtgcggc agctacctga gccgccactg gctgagtgcc gatatttttc gccgcttcaa
    40321 agccttgctg aatggtgcga ccaaataccg ctgcctggcc attcagcggc aacagcaggg
    40381 cgattttgtt ggtcgaggct ggtttaaacg cttttacgtt aaccaactgc gttggcagca
    40441 ttttcgcgcc cggattgttc ggataacgtt tttgccagtc ggcgatcccg gctttcatca
    40501 tgtcgggatc gttacggtta tcaaaccaga cgcgctgcag atccagccag ccttgcagaa
    40561 tattttcgtc ggcgttgatc accagcgtat tcgcctgttc ctgagtcatg gaggagagcg
    40621 cctgccaggt ggcatcaata ttctgctttt tttctttcgc gccgagcagc ggttcctgag
    40681 caattaacgc gcgcagtaaa tcaatggaag gacgcccctg gctggcatcg atttttgcct
    40741 gccagtaacg cgcttgctgg ttttgttcta aatcggcagg tgtgattttc gccagcaagt
    40801 tttgcgcgcc agcaaaatct ttctgcgcca gtttaatctc taccgccagc agtgttttct
    40861 cgcgacgctg agaatcgttc agttcttgcg gtagttggtt aaacaactca accgcctgcc
    40921 cggttttacc ttctttcacc agtgcacgaa tggcgagtaa ttgccagttg atcctggtat
    40981 catcagagct ttgctgcatc tgctgaagat aaaaggcaga atcagcctgc gccgtgccct
    41041 gcatataagc agtggactga tcgggagtat gggtgccaca accggcgaaa atcagggctg
    41101 ccagaacaac aggcagacaa cgcgcggctt tcaaacgaga aaatgttgag ggtaccataa
    41161 tgtatccagt gatatttttt tacgcaatgc tcaatattaa atcggcaata cggacgacac
    41221 aatgaaacaa caccaatcgg cggataattc tcagggccag ctttacattg taccgacgcc
    41281 aatcggcaat ctggcggata tcacccagcg tgcgttagag gtattacagg ccgttgatct
    41341 gattgccgcc gaggatactc gtcacaccgg tttattgctg caacattttg ggattaatgc
    41401 ccggttgttt gcgctgcacg accataacga acaacaaaaa gccgaaacgc tgctggcgaa
    41461 gctgcaagag gggcaaaaca ttgcgctggt ttccgatgcc ggaacgccgc taattaacga
    41521 tcctggctac catctggtgc gtacctgccg tgaagcgggg attcgtgtgg tgccgctacc
    41581 cggaccgtgt gctgctatca ctgcgttaag cgccgcgggt ttaccctctg accgtttctg
    41641 ttacgaaggc tttttacctg ccaaatcaaa aggccgccgt gatgcgctaa aagccattga
    41701 agcggagccg cgcacgctga ttttttatga atctacccac cgtctgttag atagcctgga
    41761 agatatcgtt gcggtattag gcgaatcccg ctacgtggtt ctggcgcgtg agctgaccaa
    41821 aacctgggaa accattcacg gcgcgcccgt tggcgagctg ctggcgtggg taaaggaaga
    41881 tgaaaaccgt cgcaaaggcg aaatggtgct gattgtcgaa ggtcataaag cacaggaaga
    41941 agacttaccc gccgatgccc tgcgcacgct ggcgctgcta caggcagaac tgccgctgaa
    42001 aaaagcggcg gcgctggccg cagaaattca tggcgtgaag aaaaatgcgc tgtataagta
    42061 tgcgctggag cagcaggggt aacagtgccg aagcgttgcg ttaatcaaaa cgcaacgtct
    42121 tcttttaccg caccatgacg gtaatggtgg cggtgccctg aaattttcct gtttccagct
    42181 caccaccgac cagattaact ggccaggcgc gcatattgac ggtgccatgc ccggaaggat
    42241 caacgggaag gataccgttc tgaaaaggaa tatttgcaac tccgccgccg agattttcaa
    42301 tacgaatacc gacatcgggc acattgtctg aacttcttcg cctggcgacc aggttgtact
    42361 ggtctactac cccagttgta ccgtcgatgc gcatttgcaa cgagttttta atctttgaag
    42421 tatcaccaca gtcataagtg atatcaaagt ctactggagt aaaaccgtct ggcatggcat
    42481 tgcgggtggt aaacttctga ccattgatga atccaaaatt aaccttaata acatcgccct
    42541 gattaatttt gcacgactgt ggggcggtga gatttccctg aatcgataac ttgactaaat
    42601 catggaagtc acctgtagac gggcttccca ttccatcgac ccacgcgcct ttaatgacgg
    42661 caatatccgt tttcggaatg gtcatcgagc cgagaaatgg cttggtgaca tacagggtaa
    42721 aggtggtatt tgcaccaata ttaacgggtc cataacgaga cgtgtcaccc tggcaaacac
    42781 ccgtttgggt tgcagcagaa tatacggtgc tgctttttat ctgtgtgaat cgggtgttaa
    42841 cagagacaac ctggtcggtt gtgagtcctg gaatgtcgtt tgcctgtaat gtggttttaa
    42901 tatccaggct gtcattgagt ttgtaatatc ctgtctgatg tccagtggtg gtaagtacgg
    42961 ggctgaccat atagacaagt ttaactgcct tacccgcaga gcaggaacag gcgtattgcc
    43021 agttagtgct acttagcgtt gcagaggta
//
LOCUS       DAKISA010000026        42200 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.026, whole genome shotgun sequence.
ACCESSION   DAKISA010000026 DAKISA010000000
VERSION     DAKISA010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42200
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.026"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..285
                     /locus_tag="OVW45_001456"
     CDS             <1..285
                     /locus_tag="OVW45_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HCV5525945.1"
                     /translation="ELVLSAGFLLVIHGATDKFAPAGFAPIAIGLALTLIHLISIPVT
                     NTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTLLEKRD"
     gene            780..1679
                     /gene="lysO"
                     /locus_tag="OVW45_001457"
     CDS             780..1679
                     /gene="lysO"
                     /locus_tag="OVW45_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCV5525946.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            1823..3475
                     /gene="hcp"
                     /locus_tag="OVW45_001458"
                     /gene_synonym="priS"
     CDS             1823..3475
                     /gene="hcp"
                     /locus_tag="OVW45_001458"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCV5525947.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKACEYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            3487..4455
                     /gene="hcr"
                     /locus_tag="OVW45_001459"
     CDS             3487..4455
                     /gene="hcr"
                     /locus_tag="OVW45_001459"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCV5525948.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            4588..6306
                     /gene="poxB"
                     /locus_tag="OVW45_001460"
     CDS             4588..6306
                     /gene="poxB"
                     /locus_tag="OVW45_001460"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCV5525949.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSLGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            6343..7344
                     /gene="ltaE"
                     /locus_tag="OVW45_001461"
     CDS             6343..7344
                     /gene="ltaE"
                     /locus_tag="OVW45_001461"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCV5525950.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRKRN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            7355..8785
                     /locus_tag="OVW45_001462"
     CDS             7355..8785
                     /locus_tag="OVW45_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCV5525951.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKASGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            8884..9897
                     /locus_tag="OVW45_001463"
     CDS             8884..9897
                     /locus_tag="OVW45_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCV5525952.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENTVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPIITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(9894..10724)
                     /gene="amiD"
                     /locus_tag="OVW45_001464"
     CDS             complement(9894..10724)
                     /gene="amiD"
                     /locus_tag="OVW45_001464"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCV5525953.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPTQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(10721..11044)
                     /locus_tag="OVW45_001465"
     CDS             complement(10721..11044)
                     /locus_tag="OVW45_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCV5525954.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            complement(11255..13024)
                     /locus_tag="OVW45_001466"
     CDS             complement(11255..13024)
                     /locus_tag="OVW45_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCV5525955.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENMPSANYQFNTFFSNAFETDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQDQLDKHFTIESHPLLYRHT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKGAFIHFYDYSRRYAISENIEKDAVTNNIA
                     LMNTFYDSDNTSGEVLNNDFTEEEPFETALDVEHTIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMRSSHYHDICSVCVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGFFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNIINNVFKNVSFRSVKINNVNTHSLKFINCHFFNVDMIRVNFSKCLFH
                     DCSMHGVKIKPWLPVKWTKELINDYLYGCLLSLYSICARDIYNMSAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQERAFIYEFARMIFAYINDKSIEILLLSCFAASDQKAIQRY
                     IPQSQDGEDFRSHLQYKLPLSAH"
     gene            13673..14188
                     /locus_tag="OVW45_001467"
     CDS             13673..14188
                     /locus_tag="OVW45_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCV5525956.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            14406..15134
                     /gene="artP"
                     /locus_tag="OVW45_001468"
     CDS             14406..15134
                     /gene="artP"
                     /locus_tag="OVW45_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCV5525957.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            15152..15873
                     /gene="artJ"
                     /locus_tag="OVW45_001469"
                     /pseudo
     CDS             15152..15873
                     /gene="artJ"
                     /locus_tag="OVW45_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
     gene            15880..16596
                     /gene="artQ"
                     /locus_tag="OVW45_001470"
     CDS             15880..16596
                     /gene="artQ"
                     /locus_tag="OVW45_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCV5525958.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            16596..17264
                     /gene="artM"
                     /locus_tag="OVW45_001471"
     CDS             16596..17264
                     /gene="artM"
                     /locus_tag="OVW45_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCV5525959.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLALLLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            17556..18287
                     /gene="artJ"
                     /locus_tag="OVW45_001472"
     CDS             17556..18287
                     /gene="artJ"
                     /locus_tag="OVW45_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCV5525960.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDSVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(18462..19589)
                     /gene="rlmC"
                     /locus_tag="OVW45_001473"
     CDS             complement(18462..19589)
                     /gene="rlmC"
                     /locus_tag="OVW45_001473"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCV5525961.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGDVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVMTLLVKQ"
     gene            complement(19630..20118)
                     /locus_tag="OVW45_001474"
     CDS             complement(19630..20118)
                     /locus_tag="OVW45_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCV5525962.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(20178..21023)
                     /gene="potI"
                     /locus_tag="OVW45_001475"
     CDS             complement(20178..21023)
                     /gene="potI"
                     /locus_tag="OVW45_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCV5525963.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWLTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(21020..21973)
                     /gene="potH"
                     /locus_tag="OVW45_001476"
     CDS             complement(21020..21973)
                     /gene="potH"
                     /locus_tag="OVW45_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCV5525964.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALLYIWLILLF
                     LLPFLIVFKISLGEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(21983..23116)
                     /gene="potG"
                     /locus_tag="OVW45_001477"
     CDS             complement(21983..23116)
                     /gene="potG"
                     /locus_tag="OVW45_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCV5525965.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(23211..24323)
                     /gene="potF"
                     /locus_tag="OVW45_001478"
                     /pseudo
     CDS             complement(23211..24323)
                     /gene="potF"
                     /locus_tag="OVW45_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
     gene            complement(24674..25150)
                     /locus_tag="OVW45_001479"
     CDS             complement(24674..25150)
                     /locus_tag="OVW45_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCV5525966.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(25238..26140)
                     /gene="rimK"
                     /locus_tag="OVW45_001480"
     CDS             complement(25238..26140)
                     /gene="rimK"
                     /locus_tag="OVW45_001480"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCV5525967.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(26201..26923)
                     /gene="nfsA"
                     /locus_tag="OVW45_001481"
     CDS             complement(26201..26923)
                     /gene="nfsA"
                     /locus_tag="OVW45_001481"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCV5525968.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(26907..27194)
                     /locus_tag="OVW45_001482"
     CDS             complement(26907..27194)
                     /locus_tag="OVW45_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCV5525969.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            27354..27611
                     /gene="grxA"
                     /locus_tag="OVW45_001483"
     CDS             27354..27611
                     /gene="grxA"
                     /locus_tag="OVW45_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCV5525970.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(27641..28018)
                     /locus_tag="OVW45_001484"
     CDS             complement(27641..28018)
                     /locus_tag="OVW45_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCV5525971.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            28288..29973
                     /locus_tag="OVW45_001485"
     CDS             28288..29973
                     /locus_tag="OVW45_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCV5525972.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(30148..30684)
                     /gene="rcdA"
                     /locus_tag="OVW45_001486"
     CDS             complement(30148..30684)
                     /gene="rcdA"
                     /locus_tag="OVW45_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCV5525973.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            30768..31976
                     /locus_tag="OVW45_001487"
     CDS             30768..31976
                     /locus_tag="OVW45_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCV5525974.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTRTAMENS"
     gene            31976..32791
                     /gene="ybjI"
                     /locus_tag="OVW45_001488"
     CDS             31976..32791
                     /gene="ybjI"
                     /locus_tag="OVW45_001488"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCV5525975.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            32877..33161
                     /locus_tag="OVW45_001489"
     CDS             32877..33161
                     /locus_tag="OVW45_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525976.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(33202..34423)
                     /gene="mdfA"
                     /locus_tag="OVW45_001490"
                     /pseudo
     CDS             complement(33202..34423)
                     /gene="mdfA"
                     /locus_tag="OVW45_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            34708..35304
                     /gene="ybjG"
                     /locus_tag="OVW45_001491"
     CDS             34708..35304
                     /gene="ybjG"
                     /locus_tag="OVW45_001491"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCV5525977.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            35362..36120
                     /gene="deoR"
                     /locus_tag="OVW45_001492"
     CDS             35362..36120
                     /gene="deoR"
                     /locus_tag="OVW45_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCV5525978.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFNPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(36167..37369)
                     /gene="dacC"
                     /locus_tag="OVW45_001493"
     CDS             complement(36167..37369)
                     /gene="dacC"
                     /locus_tag="OVW45_001493"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCV5525979.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            37616..38241
                     /gene="gstB"
                     /locus_tag="OVW45_001494"
                     /pseudo
     CDS             37616..38241
                     /gene="gstB"
                     /locus_tag="OVW45_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(38238..39395)
                     /gene="yliI"
                     /locus_tag="OVW45_001495"
     CDS             complement(38238..39395)
                     /gene="yliI"
                     /locus_tag="OVW45_001495"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HCV5525980.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFINSDKFPQWQQKLFIGALKDKDVIV
                     MSVNGDKVTEDGRILTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(39506..39889)
                     /gene="bssR"
                     /locus_tag="OVW45_001496"
     CDS             complement(39506..39889)
                     /gene="bssR"
                     /locus_tag="OVW45_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCV5525981.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            40102..41427
                     /gene="rimO"
                     /locus_tag="OVW45_001497"
     CDS             40102..41427
                     /gene="rimO"
                     /locus_tag="OVW45_001497"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCV5525982.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPSETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(41474..>42200)
                     /locus_tag="OVW45_001498"
     CDS             complement(41474..>42200)
                     /locus_tag="OVW45_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCV5525983.1"
                     /translation="SEAMEHFAYLPTGLYVFAYKKDVYLRVCTLIIFFAALVAVISGT
                     SCLYLVRRVINRGIVEKEAIINNHFERVLDGGLFFSAADVKKLYSMYNSAFLDDLTKA
                     MGRKSFDEDLKALPEKGGYLCLFDVDKFKNINDTFGHLLGDEVLMKVVKILKSQIPVD
                     KGKIYRFGGDEFAVIYTGGTLEELLSILKEIVHFQVGSINLSTSIGVAHSNECTTVER
                     LKMLADERLYKSKKNGRAQISWQ"
BASE COUNT        10409 a        10879 c        10396 g        10516 t
ORIGIN      
        1 gaactggtat tgagtgcagg tttcctgttg gtgatccacg gcgcaaccga caaattcgcg
       61 ccggcaggtt ttgcgccgat cgctattggt ctggccttaa ccctgattca cttaattagt
      121 attccggtga ctaacacttc tgttaacccg gcgcgcagca ccgcggttgc tatcttccag
      181 ggcggctggg cattagaaca actgtggttc ttctgggtgg tgccaattgt cggcggcatt
      241 atcggtggtc tgatttaccg gaccctgctg gaaaagcgtg attaattaac gcagaaggcc
      301 tggcgtaatg tcaggccttt ttttaagatg ttgttatatg ttgattcatc atcttgttgc
      361 gctcctttca tgagctaagc cacatattgc cactggcgca aggagcgcgc agggggcggc
      421 caatcgccgc cgccccctgc ccccccgggc tctggcgaac aaaatcgccg ctgcgcggtg
      481 ccctcgactt atcccttacg gctaccgggt cgggcgcgag gtaacatccc tgtaaaacgc
      541 gccctcagcc cacatccatg tgggctgccc cggccttcag ggaacgcctc ggcgattttg
      601 acgccaccaa catcggtgca gtttgtaatt aaagcgcaag aatatcttta aatgaaaacg
      661 aatactctac tgctccttat gcataacatt tccttttaat tgcaacttta ctcgtccttc
      721 cgctttcagt agtgtgtcat cgcgcatttc gacttcatct ttgcaaggac tgggttttca
      781 tgttttctgg gctgttaatc attctggttc ccctgattgt aggttacctc attccgcttc
      841 gccaacaagc tgcgttaaaa gttattaatc agctattaag ctggatggtt taccttattc
      901 tcttttttat gggtatcagt ctggcgtttc tcgataacct cgccagtaac ctgttggcga
      961 ttctgcatta ttctgccgtc agtattaccg ttattttact gtgtaatatt gccgccctga
     1021 tgtggctgga gcgaggcctg ccgtggcgca accaccatca gcaagaaaaa ctcccgtcgc
     1081 gtattgcgat ggcgctggag tcgctaaaac tgtgcggcgt agtagtgatt ggttttgcca
     1141 ttggtctgag tggactggct ttcttacaac acgcgaccga agccagtgaa tacacgttaa
     1201 ttttgctact tttcctcgtt ggtattcagt tgcgcaataa tggcatgacc ttaaagcaga
     1261 ttgtccttaa tcgccgggga atgattgtcg ccgtggtggt ggttgtcagt tcattaattg
     1321 gtggtttaat taacgccttt attcttgatc tccccatcaa taccgcgctg gcaatggcct
     1381 ccggtttcgg ctggtattct ctttccggta ttttattgac cgaatctttt ggtccggtaa
     1441 tcgggagcgc ggcgtttttt aatgatctgg cccgtgaact gattgctatt atgttgatcc
     1501 ctgggctgat tcgccgcagc cgctctactg cactgggctt atgcggtgcc acatcaatgg
     1561 atttcaccct gcccgttctt caacgtactg gcgggctgga tatggtcccg gcggcaattg
     1621 ttcacggttt tattcttagc ctgttagtgc cgatcctcat cgcctttttc tctgcgtaat
     1681 acctctctgg cggtagatcc ctgccgccaa aattgcgcta aatcaatctc ccttaaagtt
     1741 gcatgaaaaa tcccttttat ccccgcgtta agcgtcttaa ccttaaacat gtatattaaa
     1801 tataacttta aaaggtgtga tcatgttttg tgtgcaatgt gaacaaacta tccgtactcc
     1861 ggcaggaaac ggctgctcat acgcgcaggg gatgtgtggt aaaacggcgg aaacttctga
     1921 ccttcaggat ttactcatcg cggcgctgca agggctttct gcctgggcgg taaaagcgtg
     1981 tgaatacggc atcatcaacc acgatgttga cagcttcgcg ccacgtgcct ttttctcaac
     2041 cctgaccaac gttaacttcg attctccgcg tattgtcggc tacgctcgtg aagcgattgc
     2101 cttgcgcgag gcgctgaaag cacaatgcct ggctgtagat gccaacgcgc gcgtcgataa
     2161 cccgatggct gacctgcaac tggtgagcga tgatctcggc gagctgcaac gtcaggcagc
     2221 agaatttacc cctaacaaag ataaagcggc gattggcgaa aacattctcg gcctgcgtct
     2281 gctgtgcctg tatggcctga aaggtgcggc ggcctatatg gaacacgcgc atgttctcgg
     2341 tcaatacgac aacgatattt atgcccagta ccataaaatc atggcgtggc tggggacttg
     2401 gcctgccgat atgaacgcgc ttcttgagtg ttcaatggaa atcggccaga tgaacttcaa
     2461 agtgatgagc attctggatg caggcgaaac cggtaaatac ggtcacccga cgccaaccca
     2521 ggtcaacgtc aaagcgacgg cgggtaaatg cattctgatt tccggtcacg atctcaaaga
     2581 tctctacaac ctgctggaac agaccgaagg cacgggcgtt aatgtctaca cccacggcga
     2641 aatgctgcct gcgcatggct acccggagct gcgtaaattc aagcatctgg tcggtaacta
     2701 cggcagcggc tggcagaatc agcaagtgga gttcgctcgt ttccctggcc ccatcgtgat
     2761 gacctcgaac tgcatcatcg acccaaccgt aggcgcttat gacgatcgta tctggacccg
     2821 cagcattgtt ggctggcctg gcgtgcgtca tctggacggt gatgatttct ctgcggttat
     2881 cacccaggcg caacagatgg caggcttccc gtacagcgaa attccgcacc ttatcaccgt
     2941 gggttttggt cgccagacgc tgcttggcgc tgctgatacg ctgattgatc tggtgagccg
     3001 tgaaaaactg cgtcatatct tcctgcttgg tggctgtgac ggcgcacgcg gcgagcgcca
     3061 ctacttcacc gatttcgcca ccagcgtgcc ggatgactgc ctgatcctga ccctcgcctg
     3121 tggtaaatat cgctttaaca aacttgagtt tggcgatatc gaaggtctgc cgcgtctggt
     3181 agatgcaggt caatgtaacg atgcttactc agcgattatt ctggctgtca ctctggcaga
     3241 gaaactgggc tgtggtgtga acgatctgcc gctgtcgctg gtgctctcct ggtttgaaca
     3301 gaaagcgatc gtcattctgc tgacgctgct ttctctgggc gtgaaaaata tcgtcaccgg
     3361 cccgactgca cctggtttcc tgacaccaga cctgctggcg gtgctgaacg agaaattcgg
     3421 cctgcgttct atcaccactg ttgaagaaga catgaagcaa ctgttgagcg cgtaaggagt
     3481 ttgtcgatga cgatgccaac gaatcaatgc ccgtggcgga tgcaggttca tcacattacg
     3541 caagaaacgc cggatgtgtg gacgatttcc ctgatttgcc acgattacta cccatatcgc
     3601 gccgggcaat atgcactggt cagcgtgcga aactcagcgg aaacgctgcg tgcttacacc
     3661 atttcctcca cgccaggcgt gagtgaatat atcaccctga ccgtgcggcg gattgatgac
     3721 ggtgtcggct cccagtggct gacgcgcgat gtaaaacgcg gtgattatct ctggctttcg
     3781 gacgcgatgg gggaatttac ctgcgacgat aaagcagaag ataaattcct gttgctggcg
     3841 gcaggctgcg gcgtcacgcc gattatgtcg atgcgtcgct ggcttgcgaa gaaccgtcca
     3901 caggccgatg tgcgggtgat ctacaacgtg cgtacgccgc aggatgttat tttcgccgat
     3961 gagtggcgta actatccggt aacgctggtg gcagaaaata acgttaccga aggctttatc
     4021 gctggtcgtc tcactcgcga actgctggca ggtgtacctg acttagcttc acgtaccgtg
     4081 atgacctgcg gcccggctcc gtatatggat tgggtagagc aggaagtgaa agcgctcggc
     4141 gtgacgcgtt tctttaaaga gaaattcttc accccagtag cagaagcggc gaccagcggt
     4201 ctgaaattca ccaaactgca accggcacga gaattttacg ccccggttgg caccacgcta
     4261 ctggaggcgc tggaaagcaa taacgttccg gttgtcgctg cctgccgtgc gggtgtttgc
     4321 ggctgctgta agacaaaagt ggtttccggt gaatatacgg tgagcagtac aatgacgctg
     4381 accgacgccg aaatcgctga aggttacgta ctggcctgct cctgccatcc gcagggggat
     4441 ttggttctcg cataatcgcc ttatgcccga tgatattcct ttcatcgggc tatttaaccg
     4501 ttagtgcctc ctttctctcc catcccttcc ccctccgtca gatgaactaa acttgttacc
     4561 gttatcacat tcaggagatg gagaaccatg aaacaaacgg ttgcagctta tatcgccaaa
     4621 acactcgaat cggcaggggt gaaacgcatc tggggagtca caggcgactc tctgaacggt
     4681 cttagtgaca gtcttaatcg catgggcacc atcgagtgga tgtccacccg ccacgaagaa
     4741 gtggcggcct ttgccgctgg cgctgaagca caacttagcg gagaactggc ggtctgcgcc
     4801 ggatcgtgcg gccccggcaa cctgcactta atcaacggcc tgttcgattg ccaccgcaat
     4861 cacgttccgg tactggcgat tgccgctcat attccctcca gcgaaattgg cagcggctat
     4921 ttccaggaaa cccacccaca agagctattc cgcgaatgta gtcactattg cgagctggtt
     4981 tccagcccgg agcagatccc acaagtactg gcgattgcca tgcgcaaagc ggtgcttaac
     5041 cgtggcgttt cggttgtcgt gttaccaggc gacgtggcgt taaaacctgc gccagaaggg
     5101 gcaaccacgc actggtatca tgcgccacaa ccagtcgtga cgccggaaga agaagagtta
     5161 cgcaaactgg cgcaactgct gcgttattcc agcaatatcg ccctgatgtg tggcagcggc
     5221 tgcgcggggg cgcataaaga gttagttgag tttgccggga aaattaaagc gcctattgtt
     5281 catgccctgc gcggtaaaga acatgtcgaa tacgataatc cgtatgatgt tggaatgacc
     5341 gggttaatcg gcttctcgtt aggtttccat accatgatga acgccgacac gttagtgcta
     5401 ctcggcacgc aatttcccta ccgcgccttc tacccgaccg atgccaaaat cattcagatt
     5461 gatatcaacc cagccagcat cggcgctcac agcaaggtgg atatggcact ggtcggcgat
     5521 atcaagtcga ctctgcgtgc attgcttcca ttggtggaag aaaaagccga tcgcaagttt
     5581 ctggataaag cgctggaaga ttaccgcgac gcccgcaaag ggctggacga tttagctaaa
     5641 ccgagcgaga aagccattca cccgcaatat ctggcgcagc aaattagtca ttttgccgcc
     5701 gatgacgcta ttttcacctg tgacgttggt acgccaacgg tgtgggcggc acgttatcta
     5761 aaaatgaacg gcaagcgtcg cctgttaggt tcgtttaacc acggttcgat ggctaacgcc
     5821 atgccgcagg cgctgggtgc gcaggcgaca gagccagaac gtcaggtggt cgccatgtgc
     5881 ggcgatggcg gttttagcat gttgatgggc gatttcctct cagtagtgca gatgaaactg
     5941 ccagtgaaaa ttgtcgtctt taacaacagc gtgctgggct ttgtggcgat ggagatgaaa
     6001 gctggtggct atttgactga cggcaccgaa ctacacgaca caaactttgc ccgcattgcc
     6061 gaagcgtgcg gcattacggg tatccgtgta gaaaaagcgt ctgaagttga tgaagccctg
     6121 caacgcgcct tctccatcga cggtccggtg ctggtggatg tggtggtcgc caaagaagag
     6181 ttagccattc caccgcagat caaactcgaa caggccaaag gatttagctt gtatatgttg
     6241 cgcgcaatca tcagcggacg cggcgatgaa gtgatcgaac tggcgaaaac gaactggcta
     6301 aggtaaaaag ggtggcattt cccgtcataa taaggacatg ccatgattga tttacgcagt
     6361 gataccgtta cccgaccgag ccgcgccatg ctcgaagcaa tgatggccgc cccggttggg
     6421 gacgacgttt acggagacga ccctaccgtt aatgctctgc aggactacgc cgcagagctt
     6481 tccggtaaag aagccgccat ttttctgccg accggcaccc aggctaacct ggtcgctctg
     6541 ctcagtcact gcgaacgcgg cgaagagtat attgtcggtc aggccgcgca taattatctg
     6601 tttgaagccg gtggcgcagc ggtgctgggc agtattcaac cgcaacccat cgacgcggct
     6661 gccgacggca cgctaccgct ggataaagtg gcgatgaaaa tcaaacccga cgatatccat
     6721 ttcgcccgca ccaaattact cagtctagaa aacacccaca acggcaaagt gctgccgcgt
     6781 gaatacctga aagaagcatg ggaatttacc cgcaagcgca atctggcgct gcatgttgac
     6841 ggtgcgcgca tctttaatgc cgtggtggct tacggctgcg aactgaaaga gatcacgcaa
     6901 tattgtgatt cgttcaccat ttgcctgtcg aaaggtcttg ggacgccagt cggttcatta
     6961 ctcgtcggta atcgtgatta cattaaacgt gccatccgct ggcggaaaat ggccggtggc
     7021 gggatgcgtc agtccgggat tctggctgcc gccgggatgt atgccctgaa aaataacgtc
     7081 gcacggttgc aggaagatca cgacaacgcc gcctggatgg cggagcaact gcgtgaagca
     7141 ggcgcggatg tgatgcgtca ggacactaat atgctgtttg ttcgcgtcgg ggaagaaaat
     7201 gctgccgcgt taggcgaata catgaaagcg agaaacgtgc tgattaacgc ctcgccgatt
     7261 gtccgcctgg tgacgcatct tgacgtctcg cgcgaacaac tggcggaagt cgccgcccac
     7321 tggcgcgcat tcctggcgcg ttaaggagag agacgtgccg caacgcattt tagttctcgg
     7381 tgccagtggc tacattggtc agcatctggt gcgcacactc agccagcaag ggcatcagat
     7441 cctggcggcg gcacgtcatg tcgacaggct tgcaaagctg caactggcaa acgtcagttg
     7501 ccataaagtc gatctcagct ggccggataa ccttccggcc ctgttgcagg atatcgatac
     7561 cgtctatttt ctggtgcaca gcatgggcga aggcggcgat tttatcgctc aggagcgcca
     7621 ggtggctctc aacgtccgcg atgcgctacg tgaagtacca gttaagcaat taatctttct
     7681 cagttcgttg caggccccgc cacatgagca gtcggatcat ctgcgtgctc gtcaggctac
     7741 ggcggacatt cttcgtgaag cgaatgtacc tgtgaccgaa cttcgggccg gaattatcgt
     7801 tggcgcaggt tcagcggcgt tcgaagtcat gcgcgatatg gtctacaacc tgccagtgtt
     7861 aacgccgcca cgctgggtac gttcacgcac cacgcccatc gcgctggaaa acttgctgca
     7921 ctatctggtg gcgttgttag atcatccggc cagcgaacac cgcatcttcg aagccgccgg
     7981 accagaggtg ctcagttatc agcaacagtt tgaacatttt atggcggtga gcggtaagcg
     8041 ccgctggttg atccccatcc ccctccccac ccgctggatt tcggtgtggt ttctcaatgt
     8101 gattacttcc gtaccgccca ccaccgccag ggcgttgatt caggggctga aacacgatct
     8161 gctggcggat gataccgcgc tacgtgcact catcccacaa cggctgatcg ctttcgatga
     8221 cgcggtacgt agcacgttga aagaggagga aaaactggtc aactccagcg actggggcta
     8281 cgacgctcag gcctttgccc gctggcgacc ggagtacggt tattttgcca aacaggcggg
     8341 gtttaccgtt aaaacgtccg ccagccttgc tgctttatgg caggtagtga accaaatcgg
     8401 cggtaaagag cgttatttct ttggcaatat tttgtggcag acacgggcgt tgatggaccg
     8461 cgcgatcggt cataagctgg cgaaaggccg cccggagcgc gaatatttac agactggcga
     8521 tgcggtggat agctggaaag tgattgtcgt tgaaccggaa aaacaactta cgttgttatt
     8581 tggcatgaaa gcgtcggggc tgggacgact gtgttttagc ctggaagata aaggcgacta
     8641 tcgtactatc gatgtccgcg ctttctggca tccgcacggt atgccggggc tgttttactg
     8701 gttattgatg atccccgcgc atctgtttat ttttcgcgga atggcaaaac aaatcgccag
     8761 actggcagaa caaagcacag attaatcgac aaaacctgcg ttattctttc atgtttccca
     8821 ttgcatcatg ggggaaatca cggaagaatg cgcacgtcat tttctcaaca acagtggatt
     8881 gatatgaagg tactggttac cggcgccacc agcggcttag gtcgaaacgc ggtagagttt
     8941 ttatgccaga aaggcatcag cgtgcgagcg accggtcgca acgaggcaat gggcaaattg
     9001 ctggaaaaaa tgggcgcaga gtttgttccg gcggatctga ccgagctggt ttcatcacaa
     9061 gctaaagtga tgctcgcggg cattgatacg ctgtggcact gctccagctt tacctcaccc
     9121 tgggggacac aacaggcttt cgatctggct aacgttcgcg ccactcgccg cctgggtgaa
     9181 tgggctgtcg cctggggtgt gcgtaacttt attcatatct cttccccctc cctgtacttc
     9241 gattatcacc accatcgcga tattaaagaa gattttcgcc ctcaccgctt cgccaacgag
     9301 tttgcccgca gcaaagcagc cagcgaagaa gtgatcaata tgctttcgca ggcgaatcca
     9361 caaacgcgct ttactattct gcgcccacaa agtctgttcg gaccgcacga taaagtcttt
     9421 attccccgtc tggcgcatat gatgcaccac tacggcagta ttctgttacc gcatggcggc
     9481 agtgcgctgg tcgatatgac ctactatgaa aataccgtgc acgcaatgtg gctggcaagc
     9541 caggaagcct gcgataagct accttccggg cgtgtgtaca atatcaccaa cggcgagcat
     9601 cgcacactgc gcagcatcgt gcagaagctg atcgacgagt tgaatattga ctgtcgtatt
     9661 cgttccgtcc cctacccgat gctggatatg atcgcccgca gcatggagcg tttaggccgc
     9721 aagtcagcaa aagagccgcc gctgacccac tacggcgtct ccaagttgaa ttttgacttt
     9781 acgctggata ttacgcgtgc gcaggaagag ttaggttatc agccgatcat caccctagat
     9841 gaaggtatcg agaaaactgc cgcctggcta cgcgaccacg gaaaactgcc gcgctaatcc
     9901 tgcccgtatt tctccagcaa tgcttcggca atcgcctgag tttctgcatc cgcttcgccg
     9961 ttatataacg tcgggcggaa atgcatctgg aatgccataa tcacgcgccg ctgctcgcgc
    10021 ggtgtcatat caggtttaac gtcataaccg tagcgcgcca aaagctccag caatgacgcg
    10081 gtatctaccg gtgtgtgcgg cgcgcgcccg gcaaggtaaa agttaacccg ctgcgcatcc
    10141 ggccaggcac caatcccctg ctgcgccagt tgctgccagg gaaataatgg ccccggatcg
    10201 tctttgcgct gcggtgcgat atccgcatgt gccactacgt tttccggctt gatgtgataa
    10261 cgggcaataa tatctttcgc cagtggaata agcgcctgaa tctgtgtcgg ttcaaacggg
    10321 gcaaaatatt tcactccggc cgatttttgc cagccacggt tttccagctc aatgccaata
    10381 gaggtgtcgt taaggcgcgt tgccccgcgc caggcgctaa tccccgcatg ccaggccagt
    10441 tcttgttccg gcaccagttg ccagatgcgc ggtttaccgt tgtatcgcgg tggtaccgca
    10501 gggaccagat aatgcgagct gacctgctta tcggtcagtg tcgccagcga gctatcaaaa
    10561 tcatctgcgg tgtagtggat caccagcact ttaatgcgcg gatacgccgc ctgcgcctgg
    10621 cgtcgggtat caagctgata tccctctttc tcgacaatgc ctttttcgcc tgcacaccct
    10681 gccaataaca gagcggcagc gaccagccag aagactcttc tcatcgacgc gttttcaccg
    10741 ccgtaccgct gacgctaacc atcagcatac tgccgttttg cccgaccgtt tcgtagtcga
    10801 tatcaatacc gacgacggca tcggccccca gcgcccgcgc ctgggagcct aattcctcaa
    10861 aggcgatctc ccgtgcttta cgcagttctt tttcatacgc accggaacgt ccgccaacga
    10921 tatcgcggat accggcaaag aaatcacgga aaatattggc acctaaaatc gcttcgccgg
    10981 tcaccacacc gcaatattca acgatggtct ggccttccag agttggggtt gtagaaaatt
    11041 gcatgatcga tctccttgtt tgttaagcct tcgatctcta aagcattatc gaactgatat
    11101 gccatgttgg gaagaaggtt ttgatcagtt taggaaatca tctcattgtg tcaaaaacac
    11161 ccacttaagt ctttacgatt atccgttgca taacccgata ttggtttcat tatagggtac
    11221 cgcttacctg tcttattatg ttgcaccaac aacattaatg agcggaaaga ggcaacttat
    11281 attgtaaatg actcctaaag tcttccccat cttgtgactg aggtatatac ctctggattg
    11341 ctttttggtc agacgccgca aaacaagaaa gtaataagat ctcaatgctt ttgtcattaa
    11401 tataagcgaa gatcattctt gcaaattcgt aaataaacgc ccgttcctga gcgcttaata
    11461 gtttaatgca atatttattc ttaagagaga aaataatttc aaggaaagca tccgcagcaa
    11521 ccttcacgtt attcccggcg ctcatattat atatatccct tgcacagata gagtacagag
    11581 atagtaaaca gccatacaaa taatcattta ttaactcttt tgtccatttt actggcaacc
    11641 atggttttat cttgacacca tgcatactac agtcatgaaa aagacatttg ctgaaattaa
    11701 ctcttatcat atcaacatta aaaaaatggc aattaataaa ttttaaagaa tgagtattta
    11761 cgttgttaat tttaacagat ctaaacgaaa catttttgaa tacattattt attatattgc
    11821 tatttgttat tttaacatca cctaaactgc cgttttcaaa acaacatttt ttcaatatgc
    11881 tttcattcac aacagtgtgt gataagtaga gattacttat gttttgcttt attaatgcca
    11941 cgtcattaaa atgactatat ttaatgaaaa aaccatcatt gagtagtgtt ttaagattga
    12001 ctaaattact tgcatcttca cgtaccatcg tggcaactgg aacaggcaga gacatgaaaa
    12061 cttcttcatt caccatcaag tgaatcatgc attgcgttcc ctttgtcata tccacacata
    12121 cgctacaaat atcgtggtaa tgcgaagagc gcatttcagg aaacagatta aacacggtat
    12181 cttgaaaggc actaaatcgt gttaataaat cctgataatg aacaggtttt gtttcaaaat
    12241 tatcatcagc aaatccaagc actattgtat gctctacatc taacgcagtt tcaaatggct
    12301 cttcttctgt aaaatcatta tttaaaacct cacctgacgt attatcactg tcataaaaag
    12361 tgttcatcaa tgcgatattg tttgttacag cgtctttttc gatattctca ctaattgcat
    12421 atcgcctgct ataatcataa aaatgaataa acgcgccctt tataaaggat atatattgtt
    12481 ctttgttaaa gtcaatacgg ctgtataatt tttcaaaaat cacattcgca attttactac
    12541 caatggtatg cctgtataat aatgggtgcg attcaatcgt aaaatgcttg tctaattgat
    12601 cttgtaattt attctcatag tccagaggga aaagaaactg ttgtctttca agtttcccat
    12661 tcgatacatt acgtattcca atagagattc tttcattacc cgcctttatt tcaacttcgt
    12721 tatcttctga tttacccaga tattttttaa catcagtctc gaaagcatta ctaaaaaaag
    12781 tattaaattg gtaattcgct gatggcatat tttccgcgag caacacatca ttttttatga
    12841 gatcactgtc agcatttatt gctaatatca gatgcaagta gatggtatcc catgtagaca
    12901 actcaccctt acgactggaa tttaatatcg cgaaaaattc cctcagtgaa tccaggaaat
    12961 tatcactttg atttagttgt gtgcctggaa atgatgatgc ttgatcagat atattgttta
    13021 tcatattaaa aatcacccaa gaaaaacgat aatccaccga actcctcgtg ctttataatt
    13081 ataaaagcac aacgcctttt atattcatat taattaacga gatactgaaa caacttaatt
    13141 tattcttcgc taaaattcat tagcttaaat tctttaccca caattgtagc ataaataata
    13201 ttatcgagag tcaaagctgg gactatcaaa tttcgctcat tttcaaaaat acaacgtaat
    13261 tttggcaatc atcttaatcg caggatctct tatcgcatgt gaaaaatttt gtaatgatta
    13321 gacaataacc gcgttcattt atcacgcgtt ataaatgtga aactggcttc ttgcagatgc
    13381 tcttaacctc tacaggagga tttataccac catttcagaa ctatcatatt taccttaaat
    13441 gatcatttca gccatataac acgctcaacc tcacaaatgg tttacactgt ctattgtttg
    13501 ggtaatttaa gtaagtcagc tacgagcaac cttgagtgga tagccagcaa tggactcaaa
    13561 cccaatcagg tgttcagatt gtttcagaat tgtattaaaa acaatgtatt aaataatctg
    13621 atagcgtttg gtcgtagtgt gacgcagaca gcaagctaat aaggaaatga ttatgcgcta
    13681 ccgttcacta tctctactta ttccatgtgc actgctgctg agtgcctgta ccaccgtcac
    13741 gccagcttat aaagataacg gtccccgcac cggttcctgt gtccaggggg ggcctgacag
    13801 cgtggctcaa caattctatg actaccgcat tcaacatcgc agcaatgaca ttaccgccct
    13861 gcgcccctat ttgagtgaca aactggcaac ccttcttagc gatgccagcc gcgataatag
    13921 tcatcgtgaa ttgttaagca gcgatccgtt ctccagtcga accaccttgc cagatagcgc
    13981 ccacgttgcc agtgcatcga ctatccctaa tcgtgatgcc cgtaacattc cattgcgcgt
    14041 agatctcaaa cagggtgacc agggttggca ggatgaagtg ttaatgattc aggaaggcca
    14101 gtgttgggtc atcgacgatg tgcgctactt aggcggcagc gttcatgcca cggcgggtac
    14161 gctccgtcag tccatcgaga atcgctaacg ccttgatcaa ccaataaacc ccgtaaattg
    14221 tccggcattt cgtgaggaat gccgacattt atgctcgccg accaccgccc ccgttatttt
    14281 gtgctatgtt tattgaataa tgcgctttgc ttttaacttt taaagcagaa atattgcata
    14341 attattctgt caaaggtact atcttcgacc tcaatagcta tcagactgcc agtatacgag
    14401 tgtcaatgag tattcaatta aacggcatta attgcttcta cggcgcgcat caggcgctgt
    14461 tcgatatcac gctggattgc ccacagggcg aaacgctggt gttacttggc cccagcggcg
    14521 cgggtaaaag ctcgctgctg cgtgtactca atctgcttga gatgccacgc tccggtacgc
    14581 tcaacattgc aggcaaccat ttcgatttca ccaaaacacc ctctgacaaa gcgattcgcg
    14641 atttgcgtcg taacgttggc atggtgtttc agcaatacaa cctgtggccg catctgaccg
    14701 tgcagcaaaa cctgattgaa gcgccctgcc gtgtactggg gttgagtaaa gatcaggcgc
    14761 tggcccgtgc agaaaaactg ctggaacgtc tgcgtctcaa accttatagc gatcgttacc
    14821 cgctgcatct ttctggtggt cagcagcagc gtgttgctat tgcccgtgcg ttgatgatgg
    14881 aaccgcaggt actgctgttc gatgaaccga ccgccgcact ggacccggaa attacggcac
    14941 aaatcgtcag catcattcgt gagctggcag aaacgaatat tacccaggtg atcgtcaccc
    15001 acgaagttga agtggcgcgt aaaaccgcca gccgagtggt gtatatggaa aatggtcata
    15061 tcgtagaaca aggcgacgcg agctgcttta ccgagccgca aaccgaagca tttaaaaact
    15121 atctctctca ctaagattcg ggataacaac aatgaaaaaa gttctgattg ccgcgttaat
    15181 tgcaggtttt agtctttccg ccacagctgc cgaaaccatt cgttttgcta ccgaagcatc
    15241 ctatcctccg tttgaatcga ttgatgcaaa caaccagatc gttggttttg acgtcgacct
    15301 ggcacaagcg ctgtgtaaag agcctgcact ttctctaacc aggcgtttga cagcctgatc
    15361 ccaagcctga aattccgtcg cgtagaagcc gtgatggcgg gcatggatat cactccggag
    15421 cgtgaaaagc aggtgctgtt taccaccccg tactatgaca actctgccct gtttgtgggt
    15481 cagcaaggca aatacaccag tgttgatcag ctgaaaggca aaaaagtcgg cgtacagaac
    15541 gggacgacac accagaaatt cattatggat aagcacccgg aaatcactac cgttccgtat
    15601 gacagctacc agaacgcaaa actggatctg caaaacgggc gtatcgacgg cgtcttcggt
    15661 gacaccgcag tggtcactga gtggctgaaa gataacccga aactggcggc ggtgggcgac
    15721 aaagtgaccg ataaagatta cttcggcact ggcctcggca tcgcggtacg tcagggcaac
    15781 actgagctgc agcagaaact caacactgcg ctggaaaaag tgaagaaaga tggcacttac
    15841 gaaaccatct acaacaaatg gttccagaag taattcctga tgaatgaatt ttttccttta
    15901 gcaagcgccg ccgggatgac cgtcggcctt gccgtttgtg cattgattgt cgggctggcg
    15961 ctggcgatgt tctttgcggt atgggagtcg gcaaaatggc gtcctgtcgc gtgggcaggt
    16021 tcagcgctgg taaccattct gcgtggcctg ccagaaattc tggtggtgct gtttatctat
    16081 tttggctcct cgcagctgct gctgacgctt tcggatggct tcactatcaa tcttgggttc
    16141 gtgcagatcc cagtgcagat ggacattgag aacttcgacg ttagcccgtt cctttgtggt
    16201 gtcatcgctc tgtcactgct gtatgccgcc tatgcctcgc aaacgcttcg gggcgcgttg
    16261 aaagcggtgc cggtgggtca gtgggagtct ggtcaggcgc tggggctgtc gaaatcggct
    16321 atctttttcc gtctggtgat gccgcagatg tggcgtcatg cgctgcctgg cctcggtaac
    16381 cagtggctgg tgctgctgaa agataccgcg ctggtcagtt tgattagtgt gaatgattta
    16441 atgctgcaaa caaaaagcat cgctactcgt acccaggaac catttacctg gtacattgtg
    16501 gcggcggcga tttacctggt gatcaccctg ctcagtcagt acattctcaa acgcattgac
    16561 ctgcgcgcga cacgttttga gcggaggccc agctaatgtt tgagtattta cccgaactga
    16621 tgaaagggct acacaccagc ctgacgctaa ccgttgcctc gctgattgtg gcactgattc
    16681 tggcattgat ttttaccatc atcctgacgc tgaaaacgcc ggtgctggtg tggctggtgc
    16741 ggggttatat cacgctgttt accggtacgc cgctgctggt gcagatcttc ctgatttatt
    16801 acgggccggg ccagtttccg acgttgcagg agtatccggc actgtggcat ttgttgtcag
    16861 aaccgtggtt atgtgcgctg attgcgttat cgctgaacag tgcggcgtat accacgcagc
    16921 tgttttacgg tgcaattcgt gcgatcccag aaggtcagtg gcagtcctgt agcgccctgg
    16981 gaatgagcaa aaaagatacg ctggcgctcc tgctgccgta tgcctttaaa cgctcgctct
    17041 cttcttattc caacgaagtg gtgctggtgt tcaaaagtac ctctctggct tacaccatta
    17101 cgctgatgga agtgatggga tacagccagt tgttgtacgg acgcacctac gatgtaatgg
    17161 tgttcggtgc ggcagggatt atttacctgg tcgtcaacgg cctgctgacg ctgatgatgc
    17221 gtctgatcga gcgcaaagca ctggcattcg aacggcggaa ttaatgtagc tgcgtacatt
    17281 cgccgggggg gtttgtaggt ctgataagac gcgccagcgt cgcatcagac atcagcacgg
    17341 tgcctgtgcc ggatgcggcg tggacgcctt atccggccta catagtgcat aaattcttat
    17401 aatgaagacg gacaactcac taagttatcc gtcttttttt atttcattta aattatttaa
    17461 tcatatttat tgcatataaa ttcacttgat ggcattgtta tcccatgccg cagacacggc
    17521 caaaaatcat aagattgaca gacgggagtt ccatcatgaa aaagttagtt cttgccgctt
    17581 tacttgcttc ctttactttc ggtgcttctg ccgcagagaa aatcaatttt ggcgtttcag
    17641 ccacctatcc accctttgaa tctataggtg ctaataatga gattgtcggc tttgatatcg
    17701 atctggcaaa agccttgtgc aaacaaatgc aggcagaatg tacttttact aatcacgcgt
    17761 tcgacagcct gatcccgtca ctgaaattca gaaaatatga ctccgtaatc tccggtatgg
    17821 atatcacgcc cgagcgtagc aagcaggtat cgtttaccac gccctactat gaaaactcag
    17881 ccgtcgtgat tgccaaaaaa gatacctaca aaacgtttgc cgatctgaaa ggcaaacgta
    17941 ttgggatgga aaacggcacc acgcaccaga aatatattca ggatcagcac cccgaagtga
    18001 aaactgtctc ctatgacagt tatcagaatg cctttatcga tctgaaaaat ggtcgtattg
    18061 atggggtatt tggtgacaca gcggtggtaa acgaatggct gaaaaccaat ccgcaactgg
    18121 gcgttgctac tgaaaaagtg accgatccgc aatacttcgg caccggtctg ggcatcgctg
    18181 tacgtccgga taacaaagct ctgctggaaa aactgaataa cgcgctggca gcaattaaag
    18241 ctgacggtac atatcagaaa atcaatgacc agtggttccc gcagtaattg agactgatga
    18301 caaaagtaag attgcctgat gcgctgcgct tatctggccc acatcaactc tgcaatgtat
    18361 tgatttagca tgattttgta ggccggataa ggcgtacacg ccgcatccgg catgaacaaa
    18421 gcgcacctga tcagcaatct gaagccgcac ctgcggcttt tttattgctt caccagcagc
    18481 gtcatcactt catagtgcgc ggtgtgcggg aacatatcaa aaagctgtac ccgttcgatg
    18541 cggtaacctg gcagttcgcg gatatctttc gccatggttt gggcgttaca gctggagtag
    18601 ataataaaac gcggtgccat cgttgagaga taatcacaca gcggtttacc aatgccgcgg
    18661 cgcggcgggt taaccagcac cagctccggc acatccccct gagcggtggc aaactgagtg
    18721 gagtccagcg cctgaaattg caaacgcgtt aagcccagtt cagcggctga ctgctttgca
    18781 caggcaatgg cctctgatgc aatttcgatc ccggttaact gcatgtcagg cgtcgcgcag
    18841 tgtaaaccaa agccacccac accgcagaac agatcccaca tatgtttaac tggcagctgc
    18901 cgtacccagt cgcgcgcggt agcgtacagc tggctggcga ccgccggatt agtctggaag
    18961 aaactttgcg gacggatcca cagcggtacg tcattaaaac gctctgccag cgcctgttgt
    19021 tcagtcaggt agatctccgt ctccccttcc ataatcgcca tatgtaccgg ctgaatattg
    19081 acggtaataa ctttcagttg cggtagttgt tcctgtaacc acggcagcgc cttacgcagt
    19141 tgtgccagtt tggtttcaga acgcagtaca aagcgcagca tcatgccgcc atcgctctgg
    19201 ctttcagtca gcagaatgta tttcagttcg ccacgcttac gcgccacgtt gtagggcgtt
    19261 aaccccgcac gggcgataaa cggttttagc gccgcaaaaa cgggcgcaaa agaggcagga
    19321 taaagcgggc agtcacaaag gtcttccggc gtgccatcgc gatgcagcat accgagcagt
    19381 ggtttttcaa cgctaccact caccaccatt ttggctttat tacgaaaccc ttgttccggg
    19441 cctgacaccg gcgcgcacca ttcctcaacc ggaaaatcgg cgagcagatt tttaagatcg
    19501 gcggttttag cggagagttg ctctggaatc ggctgcgtta tccactgaca ggaacgacag
    19561 cgacccgcgt cgtaaagtgc gcactgcata cattgacctt cacatcatca ggggcgacga
    19621 ttatacacat tattgcaact ggaagaaccg ccgactggtc gagggaacga acagcagcat
    19681 caggatgagc atatccggca gcttctgcag catcaggcta tggaagattt cacgttttga
    19741 ttcaccggga atgctgaaca gctccggata accgtacccc agcgaagccg cccacaagta
    19801 acttgcggcg gtgatttgtg tcagcagata tagccagcgc gcccagcgac gccctttcac
    19861 cagtgaaaac gcacaccaga tctcaatgaa aaccagcacc agactgctta aaaagaccag
    19921 cgttaaactc caggtttgta cgctgcgatg aatgaactcg ccaataccgc gcacgcccag
    19981 cgtattgaaa atcatcagca cgtcgaggcc acggatcata ataatggcga gcgccgccac
    20041 ctgcaccagc gcaggcacat tcaggcgagc atgagatgaa gatgttttct taaaaaatcc
    20101 caacgtttcg tcttccatga aaacaatgcc gcggcatgcg cggcattatg tagccaggtt
    20161 ggcaaatttt agtgtcttca gccatgtctt gcacgctgga tatcgcgtat ccgctgtttt
    20221 tctgcgcgag ccatcagata ccaggcgata aatccgacaa ttccgaccgc accgagaatt
    20281 aacgttgcaa gggcgttgat ttccggattc actcccatcc gcacgctgga aaagaccagc
    20341 atcggtaacg tggtggctcc cggcccggaa acaaagctgg cgatcaccag atcatcaagc
    20401 gacaaagtaa aagccagtaa ccagccagaa atgatcgcgg gcatgatcat cggtagcgta
    20461 atgacgaaaa acactttcag cggcgtcgca ccgagatcca tcgctgcttc ttctatcgag
    20521 cgatccagtt cccgcaatct tgaagaaata acgaccgcca cataagccgt acaaaaagtg
    20581 acatgcgcca gccagatggt gagcataccg cggtccgcag gccagccaat ggcatgagca
    20641 agcgcgacga ataataacaa cagcgacaag cccgtgatga catctggcat caccagcggc
    20701 gcggtgatca taaaggcaaa accatttgat ccgcgaaacc tgccaaaacg caccagcacc
    20761 accgccgcaa tagtcccgag gatcgccgcc gccgttgccg cacaggcagc aattgtcagg
    20821 cttaaaccaa ccgcactcat catcgcgtca tcgcgcaata actcaccata ccagcgcgtt
    20881 aaccagccgg cccacaccgt caccagcttc gagctgttaa acgaatagat aaccagcatc
    20941 agcattggcg cgtagagaaa ggtaaagccc agcagcaaaa tcacaatccg ccagggcgaa
    21001 cgaactaccg gtaaattatt catccgtgtt ctcccacgct tttttgctgg tgtttgtgga
    21061 accacattat cggcacaatt agcagcagca acatgatgat cgctaccgcc gaggccaccg
    21121 gccagtcgcg gttattgaaa aactcttgcc ataacacgcg cccgatcatg atgctgtccg
    21181 ggccaccgag cagttccggg atcacaaact cgcccacagc cgggataaac accagcatcg
    21241 atccggcaat aatcccacct ttagtcagcg gcacgatcac agtaaagaac gttttcagcg
    21301 gtcgtgcacc gagatccagc gctgcttcca ccagcgaata atcaatacga atcaacgcgg
    21361 tataaatcgg cagtaccata aacggcacgt aagcgtaaac aatgccgata taaacggcca
    21421 gattggtatg cagaatggtc agcggttgat cgataacccc cagccacagc agaaaattat
    21481 tcagcacacc gttgtttttt aatattccca tccaggcata aacgcggatc agaaacgagg
    21541 tccacgacgg caggatcacc agtagtaata aaatattacg ggtcgaaggc ttactgtgcg
    21601 ccaccgccca cgccagcgga tagccgatca gtaaacagca aaatgtcgaa atcgccgcca
    21661 cctgtaacga ctggagataa gcatcaaaat agagcggatc gtcggtcagt tgcagaaaat
    21721 taccgagatt aagagtgatg gaaagttgcc cgtcagccca ctccatcagt tcggtataag
    21781 gtggaatagc gcgcgccatc tctcccaggc ttattttaaa gacaatcaga aatggcagca
    21841 gaaacagcaa gatcaaccag atatatagca acgcaatgac cagtttgcgc ccatgcttca
    21901 tttgcagctg cgacaaccac agcttaaatc cgcccggcgg tttcgactgg gcagcaggtt
    21961 caagtgtact catctttgct ccttaaaccg tcagcaccac acagctgtcc acttcccagc
    22021 acaaacgcac ttcgtcgccc caggtcggta accctttacg atgacgatgg gcgttttgta
    22081 gctgggcgct gatcatctgc ccacttttca gtcgaacgtg atacaccgaa agatcgccga
    22141 gataggcaat gtgtatcacc tcccccaccg cgaagttaca accattggcg ggcggctctt
    22201 cgcaaagcat gattttttcc gggcgcagcg ctacgtgtac cggcacgtta tcgaccactg
    22261 aagcatccgc atcgactttc agtggatgca ccagccccgg cgaatcaagc accaggccat
    22321 cttcctgacg ctctttgagt acaccttcaa agacattaac cgaaccgata aattcagcgc
    22381 tgtagcgggt agttggatgc tcgtagatct cttccggctc gccaatctgg acaaatttcc
    22441 cgcgattcat aatggcgata cgccccgcca tggtcatcgc ctcttcctga tcgtgggtga
    22501 ccatcacaca agtcacaccg acgcgctcca gaatatccac cacttcaagc tgcatccggt
    22561 cacgcagctt tttatccagc gcgcccatcg gctcatcgag cagtaatagt ttcgggcgtt
    22621 tggcaaggct tcgggccagg gccacacgct gtcgctgacc accggaaagc tgatgcggtt
    22681 tgcgtttggc gaactcctgc atgtgcacca ggccgagcat ctcatggacc cgactggcaa
    22741 tttccgcttt cggtagtttg tcctgtttca ggccaaaagc gatgttctgt tccacggtca
    22801 tatgcggaaa cagcgcgtaa gactgaaaca tcatattgat ggggcgcagg taaggcggca
    22861 cctgtgacaa atcgacgcca tcgagcatta tctgcccggc agaaggttgt tcaaaacctg
    22921 ccagcatacg cagcagcgtg gacttgccac agccggatgc gcccagcagc gcgaagattt
    22981 cacctttgta gatggtcagg ctgacatcat cgaccgcatg ctgaccatcg taggatttgg
    23041 tgaggttgcg gatttctaat agcggcgtca gcgccttacg ggttttcgcc tgcgggcgag
    23101 ggatagcgtc attcacggct gctctccggc attgaatgtg tgcaagcgca ccatgatggt
    23161 gcgaattgcc ggctggtgtg gtgcgcccgc ccggcatcta aggctgcgga ttattttccg
    23221 ctcttcactt tggtccacgc gcgggtgcgc acacggtcga ttttcggatc ctgtactttc
    23281 agagtgaata gcttcgcacg aacgtccgcc ggaggataaa tacccgggtt ctcacggact
    23341 tccgcactca ccagcggcgt ggctgcttta ttggcgttgg catagaacac atggtcggaa
    23401 atatgcgcta ctacatccgg gcgcagcagg taattcagga actgataggc ttcgtctttg
    23461 tttttggcat ccgcaggcat ggcgaataca tcaaagaacg ccatcgcccc ttcttttgga
    23521 atcgagaacg agacattcac tccattcttc gcttccttcg cgcggtttga cgcctgccag
    23581 acatcacctg cccagccgat agcgacgcaa atatcgccgt ttgccaggtc gttaatgtat
    23641 tgagatgaat ggaaataacg aatgttcggg cgcagcttta acagcagatc ggttgccggt
    23701 ccggtgtaat catccgcttt agtgctgttg ggatctttgc cgagataatt caacacggta
    23761 gcaaaaactt cttctggcgc atccaggaaa gagacaccgc agcttttcag tttttccaga
    23821 ttttcaggtt tgaggatcaa gtcccagcta tcgacgggcg cgttttcgcc cagcacagct
    23881 ttaactttat caacgttata gccaatcccg gtcgtcgccc acatataggg catagcaaat
    23941 ttattgtcgg gatcgtgttt ggcgaccagc ttcagcagct ccgggtcgag attcttccac
    24001 tccggcaatt tgcttttgtc cagcggctgg aaaactcccg cagtcaacta gcgttcaaga
    24061 aagctggcag atggaaccac caagtcaaag ccggtgctac ccgccattaa tttgccttcc
    24121 agtacttcgt tagagtcgaa aacatcgtag acgactttaa taccggtttc tttttcaaaa
    24181 ttggccaccg tgtccggggc gatataatca gaccagttat aaatgtggag tgttttttgt
    24241 tcagccgcga gcgtgccgac agagacggcc atcagagcac ccgcagccag acccgaaagc
    24301 cattttttat ttaaggcggt catatctctt tccttctgaa agttcgttaa caaacgaaca
    24361 aaagttgtca cactaaaaga tatgcaataa acgtgcatat tttttatcgg cggggtgtaa
    24421 gaaaagagaa ctctctcccg gccagcactg ctcgctatat aaaagcatcg caagaataac
    24481 tttagccagg gtttgagact atagctcgga ttaaaaaacg gtatttaaca aatttttatg
    24541 cgtatttgct ctatgtgata aagccaaatc gacacaagag aagtgaaata atctttaaga
    24601 aaaggttaaa aacagcagat aattttgcag cgcgcagcca ctgcaccagc gactgcgtgc
    24661 tcgaaaggag ttatcagtgg aggaaatagt tttcggtaac gttgttttgc ccttcatcgt
    24721 cagtcggtaa cagcagttga tgggcattag cttcaagaat gaccatcgaa atctgctctt
    24781 cgctctgacg cacgaaccat gcaaactgct cataggttac gccctgcata acggataaag
    24841 actggcaaac caccagcttt ggcagattat catcctgcat atcaagaaat gctttcacgg
    24901 tcagcgaact ggcattgatg gcagataaat ccgccgccag cggcaatacg gctgaaggtc
    24961 ggacttccgc catggcagaa aacaggatcg tgttatcgat cagatcgatt ttggcatcaa
    25021 agacaccgtc gaaattctgc atatggggca gatgcagagc ctgacagtta tcacattcaa
    25081 aaaaactcat ccccaggtca tcgagccatt gacgcagcgt atccagacca ggaacgacca
    25141 gcgatgtcat aattttgtac aacctctttc gataaaaaga ccggcacagc ttacgcaaaa
    25201 agcgcaggca aaaccatgat cagtaatgtg attgcgacta accacccgtt ttcaggcaat
    25261 attctgtcgt agcgtggcgt tcgatccagc ggatcatttt acccgcgatg tcgataccag
    25321 tggttttttc tattccttcc agccccggcg acgcattcac ttccatcacc aacggcccgc
    25381 gattagcacg cagaatatcc acaccagcaa cgtccagcgc catcgttcgc gcggctttta
    25441 tcgcgatttc acgctcctgt ggtgtgatac tggcgacgct tgccgcgccg ccacgatgca
    25501 aattggaacg aaaatcgccc tctttcgccc gccgttcaat cgcagcgacc acttcatcgc
    25561 caacaaccag acagcggata tcgcaccctt gcgcctcttt gatatattcc tgcaccagaa
    25621 tatgcgcgtt cagaccgcgg aaagcgtcaa tcacgctttc cgccgcctga cgcgtctccg
    25681 ccagcacgac gccaattccc tgcgtgcctt caaccaactt gaccaccagc ggcgcaccac
    25741 cgaccatgtc gattaaatcg ctggtatcat ccggcgaatg cgcaatgccc gtgacaggca
    25801 ggtcgatgcc ctgacgcgcc agcagttgca tggaacgcaa tttgtcacgc gcccgggcaa
    25861 tggcgaccga ctcattgagc ggatagctcc ccagcatctc gaactggcgc agtgccgccg
    25921 tcccataaaa ggtaatggcg gtgccaatac gcgggatcac tgcgtcaaaa tggggtaact
    25981 tgcggccttt gtagtgaata gaagacgccg caggatttat gttcatgtag caagaaagcg
    26041 gatcaagaat ttcaaccagg tgaccgcgct gtatagcggc ttcacgcagc cgcttacacg
    26101 aatagagcgt tccatcccgg gacaatatgg caattttcac cctgcacctc tctgtcagac
    26161 ctggtcaaaa gcccgcgtat catacatcga cgtggcggtt ttagcgcgtc gcccaaccct
    26221 gtttgtgcaa ataatccaga ataaatgggc ggctttcttt aatgattgtt cggcggatat
    26281 gatcgctcca ggtatcccgg cgattattgc tgccacgggt gaggtaatat tccgccagtt
    26341 gctcgtcata ctgcgccagt gcgtctttat ccagcggttg atagctgttt tcatgcacca
    26401 aaatggaggc cggtaaacgc ggcttgagat ccggattatc cgcaggccag ccaaggcaca
    26461 gcccaaacag cggcagaaca tgctgcggta atttcagcag tttcgtcacc gcttcaatat
    26521 tattgcgcag gccgccgata tataccccgc ccaatcccag cgattccgct gcggttaatg
    26581 cattctgcgc catcattgcc gtatcaacga caccgagcaa cagttgttcc gccaggccga
    26641 gctgagcatc cggacagatc tgtaaatggc ggttaaagtc ggcacagaac acccagaact
    26701 ccgccgcttg cgctacgtgt ttttgcccgc cggtcagcgt caccagttct tcacgtaatg
    26761 ctttgtcggt aatgcgaata atgctactac actgcaaaaa actggaactg gacgtcgcac
    26821 gggcgctgtt aataatcgcc tcacactgcg cttcggaaat gggttcatca gtgaaatggc
    26881 gaatggagcg atggccacaa ataagttcaa tggttggcgt cattatcttt ttctctttct
    26941 gaacgtgaat attgcggtgg acggttcatc agctgtgggg caagacgttt tgccacctga
    27001 agaataacca ccaccgcagc gggaagcatg agcaaaacac cgagaaaaat catcagaatc
    27061 tgcacttctg gccgagaaaa tggctcaggc agcgacaggg agtcgcttac cgacagcagc
    27121 gccaccgcca gtagcatcat tccgataaat tccagtatca acacgccttt aggcaattta
    27181 ccgatcgcgc gcatacgctt ccctctgcaa agtgagcctt cagtctaaaa cttttcactg
    27241 tattgtgttt aacagttata gcttttagca attaatgcaa caggttaaac ctactttcag
    27301 cgaatacatt ttagcgtgat cattacaggc ataaatctat gaggagagaa ataatgcaaa
    27361 ccgttatttt tggtcgttcg ggttgccctt actgtgtgcg tgcaaaagat ctggctgaga
    27421 aattgagcaa tgaacgcgat gattttcagt atcagtatgt agatattcgt gcggaaggga
    27481 tcactaaaga agatctacaa caaaaggcag gtaaacccgt agaaaccgtg ccgcagattt
    27541 ttgtcgatca gcaacatatc ggcggctata ccgattttgc tgcatgggtg aaagaaaatc
    27601 tggacgcctg atcgtctgac aagccctcgc gttgagggct ttactgattt tttctgtgct
    27661 gtggtttaaa caaactactg ataaataaga aacacagtgc ccccagcgca caccagaaca
    27721 ccgcgcttag taaccatgcc agctcttgcc agaatgagcg cgtcggtgaa aaaaacagcc
    27781 gcataatgag cattgaacag ggtgccgcca gcattgcgcc aaatagaggt ttcaggactt
    27841 ctctacgctg tgaaaagaaa ctggcaactg ctccaggaag aatgaaaaac agcaagccga
    27901 tttcaggatg cccggcagcc cgaaaagcgc ctttcatgtg cgtcgccaga aaaaggcaca
    27961 ccacaatgaa gaggacaaaa cagcagattg cccccgccca acgttgttta tgtttcactc
    28021 gttcctcctg acactgcgtc tatcgaacac atttttcgcc agtgtggcgt tcagtaagat
    28081 aaagccgctt cgcattccat gctaatatag gccaacgcaa ttcatatagc cgttgatacc
    28141 taatgtgatt acactagtaa aatatattgt tactttacta tcgtttaggt gcgctgaatg
    28201 aatctgcgcc ctgaattctg gtaaaaaaca ttatcgtaaa ttaccatttc tttcaacagc
    28261 ttactagtaa acaagaagtt agcctccgtg aatataaacg tcgccgaatt gttaaatggg
    28321 aattacattc tgttattatt tgtggtcctc gcgcttgggc tatgtctcgg aaagttacga
    28381 cttggttcga tccaactggg taattccatt ggcgttttag tcgtatcgct gttattaggc
    28441 caacaacatt tcagcattaa caccgatgcg cttaatcttg gctttatgct gtttattttc
    28501 tgcgtcgggg tcgaagccgg accgaacttt ttttccattt tttttcgcga tgggaaaaat
    28561 tacctaatgt tagcactggt gatggttggc agtgcgctgg tgatcgcctt agggttaggt
    28621 aagctgtttg gctgggatat tggcctgacg gccggtatgt tagcaggctc tatgacgtcg
    28681 acaccggttc tggtcggtgc tggcgataca ctgcgtcatt ccggcatgga aagcaggcag
    28741 ctctcactgg cactggataa tctgagcctc gggtatgcct taacctattt aatcggtctg
    28801 gtgagtttga ttgttggtgc gcgttacttg ccgaaattgc agcatcagga cttacagacc
    28861 agcgcccagc aaatcgcccg cgaacgtggc ctggacactg atgccaaccg taaggtttat
    28921 ttaccggtga tccgcgccta tcgcgtcggc ccggagctgg tggcctggac cgacggcaaa
    28981 aatctgcgtg aactgggtat ttatcgacaa accggctgct acattgaacg tattcgacgt
    29041 aacgggattc tggcaaatcc agacggtgat gccgtgctac aaatgggcga tgaaatagcg
    29101 ttggtaggct atcccgacgc ccatgcccga ctcgatccca gcttccgtaa cggtaaagaa
    29161 gttttcgatc gtgaccttct cgacatgcgt atcgtcactg aagaagtggt cgttaaaaac
    29221 cataacgctg taggtaaacg tctcgcacaa ctgaagttga ccgatcacgg ttgcttcctt
    29281 aaccgcgtca ttcgtagcca gattgagatg ccgatagatg acaacgtcgt gcttaacaaa
    29341 ggtgacgttt tacaagtcag cggtgatgcc cgtcgcgtaa aaaccatcgc cgatcgcatc
    29401 ggctttatct cgattcacag ccaggtcact gacctgctgg cattttgcgc cttctttgtt
    29461 attgggctga tgatcgggat gatcaccttc cagttcagca cattcagttt cggcatgggg
    29521 aacgctgccg ggttgttatt cgccggaatt atgctgggct ttatgcgtgc taaccacccg
    29581 accttcggtt acattccgca aggtgcatta agcatggtga aagagttcgg cttgatggtg
    29641 tttatggcag gcgttggtct gagcgccggt agcggtatta ataacggcct gggcgcgatt
    29701 ggcggtcaga tgttgattgc cggattaatt gtcagtcttg tgcccgtggt tatctgtttc
    29761 ttgttcggtg cttatgtatt gcgaatgaac cgcgcactgt tgttcggcgc aatgatgggc
    29821 gcacgcacct gcgcgccggc aatggagatc atcagtgata cagctcgcag taacatccct
    29881 gcgctgggct atgcgggcac ctacgcaatc gccaacgtcc tgctgacgct ggcagggaca
    29941 atcatcgtca tggtatggcc aggattagga taaaactgaa gttgccctga aaatgaaatt
    30001 tttttgcaca accgcagaac ttttccgcag ggcatcagtc ttaattagtg ccactgcttt
    30061 tctttgatgt ccccattttg tggagcccat caaccccgcc atttcggttc aaggttgatg
    30121 ggttttttgt tatctaaaat ttatctacta ccctgcaacc ctctcaacca tcctcaaaat
    30181 ctcctcgcgc gatagcggct tacggtcggt gacaaaatgc agcgtcatcc cctcaataaa
    30241 cgcatcaagc gcgcgggcgg ttccgggttc aaaccattgt tcgagcgttt gctgactgcg
    30301 ctgcatccag ttttgcatta ccgtttttaa tagcggtttt cggctagcca gcgcgtagag
    30361 ctggtacatc agctccatat tatccggcgt tgcaacctgt gagctgtaga tcatatcggt
    30421 gatagcctgg catgcgcctt gagcatcact aacatcgcta aaaaatgcct gatattgccg
    30481 ggacataatc tcagtaaaac tgctgaacgc ctccagcaac aactcatcaa ttcctgaaaa
    30541 atagtaggtc atcgatccca acggtacccc ggcaagggta gcaattttgc ggtgcgtaac
    30601 agcatgtatt ccgtaaagtt tcaccgcctc cagcgtggcc tggataattt tttctcgccg
    30661 ttgcggatcg ttagcgcgac gcatgtcatt tcctctgtct gcaattgtgt acaaatgtac
    30721 acaaccttgc taatgttgtc ttccctcttc ttattttgac gtgatctatg accgtaaatt
    30781 cttcacgtaa tgcattgaaa cgccgaacct gggcgctgtt tatgttcttc tttttgccag
    30841 gcctgttaat ggcgtcctgg gcaacccgta cgcctgctat ccgcgatatt ctctctgtct
    30901 cgatcgctga aatgggcggt gttctctttg gtctgtcgat cggttcgatg agcggtattc
    30961 tctgctcggc gtggttagtg aaacgctttg ggacacgtaa tgtcatcctg gtcacgatgt
    31021 cctgcgcatt gatcgggatg atgatattaa gtctggcact ctggctgaca tcgcccctgc
    31081 tctttgccgt tggtctcggc gtctttgggg caagttttgg ttctgcggaa gtggcgataa
    31141 acgttgaagg tgccgccgtt gagcgagaaa tgaataaaac ggttttgccg atgatgcacg
    31201 gtttttatag cctaggcacg ctggcaggcg ctggtgtcgg gatggcactg acggcctttg
    31261 gcgttccggc aacggtgcac attttattgg cggcgctggt aggtatcgcg cctatttata
    31321 tcgccattca ggcaatccct gacggtacgg gcaaaaatgc tgccgatggc acccagcatg
    31381 gcgaaaaagg cattcctttt tatcgcgata tccagttgct gttgattggt gttgtagtgc
    31441 tggcgatggc ctttgccgaa ggttctgcca acgactggtt acccttatta atggttgacg
    31501 gtcacggttt tagccctact tccggctcgc tgatttatgc cggttttacc ctagggatga
    31561 ccgttggacg cttcactggc ggttggttca tcgaccgtta cagtcgcgtt gccgtggttc
    31621 gggccagtgc actaatgggg gcgttgggta ttgggctgat tatttttgtc gatagcgcct
    31681 gggtcgctgg ggtgtctgtt gtactctggg gactgggtgc ctcgctgggc ttcccgctga
    31741 ccatttctgc cgccagcgat accggccccg atgcaccaac ccgcgtcagt gtggtagcaa
    31801 cgaccggtta tctggctttc ctcgtcgggc cgccgctgct gggctatctc ggcgaacatt
    31861 atggattacg tagtgcaatg ctggttgtac tggcgctggt tattctcgcg gctattgtcg
    31921 cgaaagccgt cgccaaaccc gataccaaaa cgcggacggc gatggagaat agttgatgag
    31981 cattaaatta attgcggtag acatggatgg tactttctta agcgatcaaa aaacctataa
    32041 ccgtgagcgg tttatggctc agtatcagca aatgaaagca caaggcattc gctttgtggt
    32101 cgccagcggg aatcaatatt atcagttgat ctctttcttc cctgaaattg ctaatgaaat
    32161 tgcctttgtg gctgaaaacg gcggctgggt agtgagtgaa ggcaaagatg tttttaatgg
    32221 cgagctgtcg aaggatgcct ttactactgt cgtggaacat ttgctgacgc gcccggaagt
    32281 ggaaattatt gcctgcggaa aaaatagcgc ctatacgctc aaaaaatatg acgatgccat
    32341 gaaaacggtg gcagaaatgt attatcaccg tctggaatac gtcgataact ttgacaactt
    32401 agaagatatt ttctttaagt ttggcctgaa tctttccgat gaactgattc cacaagtaca
    32461 aaaagcatta catgaggcca tcggcgatat tatggtgcca gtccacaccg gcaacggcag
    32521 catcgatctg attatccccg gcgtacataa agccaatggc cttcgccaac tgcagaaatt
    32581 atggggaata gacgacagcg aagtggtggt ctttggcgat ggcggtaacg atattgagat
    32641 gctgcgtcag gcaggcttta gttttgcaat ggaaaatgcc ggcagcgcgg tcgtcgcagc
    32701 ggcaaaatac cgggcaggct ccaataaccg tgaaggcgta ctggatgtga tcgataaagt
    32761 gcttaaacac gaagcgccat ttgaccaata aaaacggcag cacgcccggc aacacaatac
    32821 ctgaatgaag gctgcataat tcgcggaaat taacccgctg cattatttca ggcgaaatga
    32881 ttatgaaaaa ttgtctactg ttgggcgcgc ttttaatggg ctttactggt gtggcgatgg
    32941 cgcaaagtgt caccgtcgat gtgccgagtg gatacaaagt ggttgtagtt cctgattcag
    33001 tcagcgttcc acaagcggtc agcgtcgcga ctgtgccgca aaccgtctat gtggctcctg
    33061 cgccagctcc ggcttaccgc ccgcatcctt atgctcgcca tcttgccagc gtaggtgaag
    33121 ggatggttat cgaacaccag attgacgacc atcaccatta attcgagaat gcctgatcgc
    33181 acaaagcagt caggcatttt tttacccttc gtgagaattt cccatctgtt tatcttttaa
    33241 aaagataacc atcagcgaca gccacaaaat tccgttgaca aggttgaaga gattaaactg
    33301 tccgttgccc ccgttcagcc aggcatgttt gctgatttca ataccaacgg taaagatcag
    33361 catttgcagc attcccatcg ccgcagaaac cgtaccttta ctcatatcgc tggcaaacag
    33421 ggttaatcgc accagtcccg cattcgccag accaataccg aaagcataaa tacttaaccc
    33481 ggcagtcatc cataaatacg cgtgcgatga gataaccgtt gccgcagcag cgaccaacag
    33541 accaatcata atcggccagc cgcccataat tatcacgtac ggtgcggcgc gaggtcagac
    33601 gcgctaacag caagttaccc gcaattaacg ccccgaaaat aggcacttgc agcaagccat
    33661 attcatagct gctcaactgc tccccggtaa tgatgataat cggcgactgg gcgatccacg
    33721 ccagcaatgg caggctaacg aatcccagcg cccccgccac aaagcggccg ttcttcagca
    33781 ccagcttata gtcacgaccg agttctttca gtgacagttt ctcgcctata cgcgtggcgg
    33841 tttcaggcat agctcgttgc agaccgaaaa aggagatcgc tgccaatgcg gcaaacaaaa
    33901 caaacatccc ctcccagggc agcacatgga tccacgccgc gcccaccagc ggaccaagta
    33961 gcggagcaat cagcgccacg ttcgccatca gcgcggtgat cttgatacaa accgcctctt
    34021 cgaaggattc ctgaattgcg gcgtatccca cagcgccaat gaaacagagg cttatgccct
    34081 gcaagaagcg caacagggtg aattgttcaa tgttttgcgc cagtaatatt gccagacagg
    34141 tgacgataaa ccacaccaca cccgccagca tcaccggacg gcgaccaata cgatccgaca
    34201 gcggccccag gagccattgt aaaaacatcc cgcccgccag atacgcggtc atcgaagtag
    34261 gaacccaatc aatgcccgcc tgatattgtt ccaccacggc caacataccg ggttggatca
    34321 tatcgttgcc gatataggtt gaaaattcgt aaagcaccag acagagaggg aaaagtaacg
    34381 cctgacgtcc aagcctggca ccgaaagcta atttattttg catgcaattt cttcgccaat
    34441 aataatcgcg cagagtttaa taaaagcgca gctaacgaga aagcgaattt tgtagctgaa
    34501 accacggtta agcacattct tacattattg cgagtatagc tacgctttct ttaagtttta
    34561 tttaacctat gcccgttaca atcacccacc gtaaacaggc cgcttgaggg aaataagacg
    34621 atgccgcttt acccagttta acctgcactt tattctcaac gacttgcctg tattggctcc
    34681 cttttaatca ctttgcgtcg ggaagttatg ctggaaaatt tgaatctctc tctattctct
    34741 cttattaacg cgacgccaga ctcggctccg tggatgatct cgttggcgat ttttattgct
    34801 aaagatttga ttaccgtggt gccgttgctg gccgcggtac tttggttgtg ggggcttaca
    34861 gcacaacggc aactggtgat aaaaatcgct atcgcgctgg cggtcagcct gtttgtgtcc
    34921 tggacgatgg gacatctttt tccgcacgac cgaccctttg tcgaaaatat cggctataac
    34981 ttcctgcatc atgcggcgga tgactcattc ccgagcgatc acggtacggt gattttcacc
    35041 tttgcactgg catttttatg ctggcatcgc ctgtggtccg gctcactttt aatggttctg
    35101 gccgtcgtca ttgcctggtc gcgcgtttat cttggcgtcc actggccgct ggatatgctc
    35161 ggtggattgc tggcaggtat gattggctgc cttagtgccc agattatctg gcaagcgatg
    35221 gggcataaac tctatcaacg tctgcaatcg tggtatcgcg tctgttttgc attaccgatc
    35281 cgcaaaggct gggtgcgtga ctgatctctg ctaaaaagtg tagtattgag cggctcgctt
    35341 caataactat tcagagggat tatggaaaca cgtcgcgaag agcgtatcgg gcagctgctg
    35401 caagaattaa aacgcagcga taagttacat cttaaagacg ccgccgccct gcttggggtt
    35461 tcggagatga cgattcgtcg cgatctgaac aaccacagtg cgcccgtcgt tttgctcggc
    35521 ggctatattg ttctggaacc gcgcagtgcc agccattacc tgttaagcga tcaaaaatcc
    35581 cgcctggtgg aagaaaaacg ccgggcggca aaactggctg cgacactggt agaacccgat
    35641 cagaccctct tttttgactg tggcaccacc acgccgtgga ttattgaagc gattgataat
    35701 gaaatccctt ttaccgccgt ttgttattcg ctaaatacct ttctggcgct gaaagagaaa
    35761 ccccattgcc gcgcatttct gtgcggtggt gaatttcacg ccagcaacgc cattttcaac
    35821 cccatcgatt ttcagcaaac gttgaataat ttttgcccgg atatcgcttt ttattccgcg
    35881 gcgggcgtgc atgtcagtaa aggcgctacc tgttttaatc ttgaagagtt gccggtaaaa
    35941 cactgggcca tgtcgatggc gcaaaagcat gtgctggttg tcgaccacag taaatttggc
    36001 aaggtgcgtc cggcgcgcat gggtgacctg aaacgctttg atattgtggt gagtgattgt
    36061 tgcccggaag atgagtatgt gaagtacgcg cagacgcacc gtattaagtt gatgtattaa
    36121 tgacgtataa ccggatgacg tttcgcgcca tccggttatc agaagattaa gagaaccagc
    36181 tgccgaacca ctgatggaat ttcatcatca cgaaatccca catccgacca aagaatccgc
    36241 cctcttccac attttccatc acgatcagcg gacgctgctc aatggattta ccgttaagct
    36301 ggaaatcaat ggtcccgaca acctgacctt ttttcagcgg tgcggtaagc tgcggttccg
    36361 ttaacgtgta gctcgccttc aggtttttca gctgcccacg cggtatggtc acggagcccg
    36421 cttcgcctgc cccgagattc acttcgctct tatcaccaaa ccagacgcgc tgagtcacaa
    36481 aggtggcatc aggtttaatt ggcgtcacag tttcaaagaa gcggaaaccc caggtcaata
    36541 atttctcaga ctcattaaaa cggatacggt cagttttcgc ccccagcact acggagatta
    36601 aacgcatatc accctgggta gccgaagcaa ccagattata tcccgcacct gccgtggttc
    36661 ctgtcttcat gccatcaaca ttcagattgc tgctccacag cagacggtta cggttaggct
    36721 gacgaatttt gttgaaggtg aactctttct ctttatgaat ggcgtactct tccggcacat
    36781 cgtggatcaa tgctttaccc agcaatgcca tatcgcgcgc ggtactgaac tgccccggcg
    36841 catccagacc gtgcaccgtc tggaaggtag tgttggtcag acccagtttt ttggcataac
    36901 cattcatcaa accaataaat gactcctggc tcccggcaac gtaatcggcc agcgcaatac
    36961 aggcgtcatt accggactgg ataatcacac ctttgttcaa gtctgccacc gaaacctgat
    37021 cgcccggttt gaggaacatt accgatgaac cacgcagtgc cggatttccc gtcgcccagg
    37081 catctttacc gaccgtcacc atatcggtga gtttaatctt atcggcctta agcgcctgcc
    37141 caaccacata gctggtcatg attttagtca ggctcgcggg atccagtttc tcatccgcgt
    37201 tgccttctgc cagcacttta ccgctggcgt aatccattaa aatccatgca cgcgcatcca
    37261 cgctcggcgc ttcaacggtt tgttccgccg cgaatgccgt tggggcaaaa aggaataaaa
    37321 atgcagaacc cgctgcaaga ccacgaagga gagaggagta ttgcgtcata aatgccaccc
    37381 gagtatccat tcaaaaaaaa tacgttatat cgccgtatga taagttcggt gattaataac
    37441 gcacacacaa acttaaagaa accgtaagtt ggtaaagttt ttaaagttta tgcaatatcc
    37501 tgccgcttcg ctgcttctgg tgtcattttt ttgccagctg ttgcttaaaa gttaacttta
    37561 cttcaccatc ggagtcctgt tcagcctgtg gctgcctgtc ggaaaagggg tgagtatgat
    37621 tacgctgtgg ggtcggaata attcaactaa cgtaaaaaag tattgctgac gctcgaagaa
    37681 ctggaactac cttatgagca aattctcgcg ggccgtgagt ttgggataaa tcacgatgct
    37741 gattttctgg cgatgaaccc taacgggctg gtgccgttgt tgcgtgacga cgaaagtgat
    37801 ctcattcttt gggaatcaaa cgccattgtc cgctatctgg cggcacagta cgggcaaaaa
    37861 cgcctgtgga tcgactcacc ggcacgtcgt gcggaagcgg aaaaatggat ggactgggca
    37921 aaccagacgc tcagtaatgc tcatcgcggg atcctgatgg gattagtcag aacaccaccg
    37981 gaagagcgcg atcaggccgc cattgatgcc agttgcaaag agtgcgacgc cctgtttgcc
    38041 ctgctcgatg cggaactggc aaaagtaaaa tggttctctg gcgacgagtt tggtgtgggc
    38101 gatatcgcta tcgcaccgtt catctacaat ttgtttaacg ttggcctgac ctggacaccg
    38161 cgtccaaatc tgcaacgctg gtatcagcaa ctcactgaac gccccgcagt gcgcaaagtg
    38221 gtgatgattc ccgttagcta attgcgtggg ctaactttaa gtaattcccc actggactcg
    38281 tcggtgagaa cgtataaata accgtcgggt ccagtgcgaa catcacgaat tcgctgccct
    38341 ctgtccgtta aaatacggcc atcttctgtc actttgtcgc cgttgacgct catcacaatg
    38401 acatctttat ctttcagcgc gccaataaat aatttttgct gccactgggg gaatttatcg
    38461 ctgttaataa ataatttttg ctgccactgg gggaatttat cgctgttata gaaggccatg
    38521 ccgctcacag cgggcgaatc tttccagtaa aaaacaggtt gctcggtccc ggcgacgatc
    38581 tcccctttcg cttccggtat cttaaagcct gaatagttga ttccccaggt tgccagcggc
    38641 cagccgtagt ttttgccttt ttgcgggata ttaatttcat caccaccgcg cgggccatgt
    38701 tcattcagcc acagtgcatt actccacgga ttcatcgcca ttccttgcgg attacgaatg
    38761 ccataagacc agatctcggc gcgcgcaccg gattccttta taaaaggatt atcatccggg
    38821 atttcgccct ggtcggtcag acgcaccagt ttgccctgta atttatccag atcctgcgcc
    38881 gtcgggcgct gattgttttc gcccagagca ataaaaagat aacctttacc gtcgaatacc
    38941 agccgcccgc caaaatggtt gccggtagac agttttggca tctggcggaa gacggtgcgg
    39001 aagtcggtca cttttgagag atcatcactt aagcggccat aacccacagc agttccggct
    39061 ttgccatcat cgccaacttc ggaataactt aaccagatgc ggcgagactg agcaaaatca
    39121 ggcgctaaaa ccacgtccag caggccgccc tgcccgtgcg cccaaacgtc cggaactccg
    39181 gaaagcggcg cagataatcc ttttcctgct tgccagtgac gcaactcgcc gccgcgcaga
    39241 gtgattaaca taccgtgatt atcgggtaaa aaggccagtg cccagggatg gtcgagtttg
    39301 tcttgcagta cttcgacatt taccgttgca ggagccgccc agagagcgga agaaagacaa
    39361 ataaggggca caaggaaaaa ggattgtcga tgcataacgc ctccggattg actctggctt
    39421 aaagcgtagt cagtggagga gataacaaat tcatttttac aaaaacttaa acatgaaggg
    39481 ggagacgctt tctccccctt agttttcagg ccttctcaag catggcgtgc ttctgcaggc
    39541 tctggatact cagcgttaag ctcatcagac aattttcaag cttatcggcg ttgacggtaa
    39601 taacagtcgg gcaatcatgg tgcccactca tcaaacatac tgcggctgtc gctaatgctt
    39661 cttcagcatg atgaagagca ctccactctt cctgatccag atgaagattc aaccgcagcg
    39721 atttatcgtg cagttcgcga ttcagtttaa aaaagttatc tcgtagatga ttgctttcgc
    39781 tgacggacat gtatcctttt gccttcctca gttgcacgta tgcagcgagg tcaacgcgtg
    39841 cgtcgatcaa ccggttcagc agatcgattc gctgtctgtc aacgaacata cttcgatcct
    39901 cctctttcca gccttgttgc gattaaatag tatggctatt ttttgagcaa aatgctgtcc
    39961 aggatcaatt cttcacgcca aattaacaaa atttacattt ccttcgcctc cgccagcgaa
    40021 tgagattgcg atacaaattt tatccagaac tctgtagaat ccctgccctg acctttcctg
    40081 taactgaata ttttttaagc tatgagcaaa gtaactcccc agccgaaaat cggctttgtt
    40141 tcccttggct gtccgaaaaa ccttgtcgat tcagagcgta ttctcaccga actacgcact
    40201 gaaggttatg acgtggtacc gagctatgac gatgcggaca tggtgatcgt caacacctgc
    40261 ggctttattg acagcgcggt acaagaatca ctggaagcca ttggtgaagc gttgaatgaa
    40321 aacggcaagg taattgtgac cggttgtctg ggggcaaaag aagatcagat ccgcgaagtc
    40381 cacccgaaag tgctggaaat caccgggcct catagctatg agcaggttct ggagcacgtt
    40441 catcactacg tgccaaaacc gaaacacaac ccattcctga gcctggtgcc agaacaaggt
    40501 gtgaaactga caccgcgtca ttatgcctat ctgaaaattt cggaaggctg taatcaccgc
    40561 tgcaccttct gcattattcc gtctatgcgc ggcgacctgg tgagccgtcc gattggcgaa
    40621 gtgttaagtg aagcgaaacg tctggtagat gcgggcgtta aagagattct ggtgatctcg
    40681 caggatactt ccgcctatgg cgttgatgtt aaacatcgta ctggcttcca caacggcgag
    40741 ccggtaaaaa ccagcatggt cagcctgtgc gaacagttat cgaaactggg gatctggaca
    40801 cgtctgcact acgtttaccc ttatccgcat gtggacgacg tcatcccact gatggcagaa
    40861 ggcaaaatcc tgccgtatct ggacattccg ttgcagcacg ccagcccgcg cattctcaaa
    40921 ctgatgaagc gtccgggttc tgtagatcgc caactggcgc gcatcaaaca gtggcgcgaa
    40981 atctgcccgg aactgaccct acgctcaacc tttattgtcg gcttccctag cgagacggaa
    41041 gaagatttcc agatgctact cgacttcctg aaagaagcgc gtctggatcg cgttggctgc
    41101 tttaaataca gcccggttga aggtgcagac gccaatgccc tgcctgacca ggttccggaa
    41161 gaagtgaaag aagaacgctg gaaccgtttc atgcagctgc agcagcagat ttccgccgag
    41221 cgcctgcaag agaaagtggg ccgtgaaatt ctggtgatta tcgacgaagt ggacgaagaa
    41281 ggcgcgattg gtcgcagcat ggcagatgca ccggaaatcg acggcgcggt ttatctcaat
    41341 ggtgaaacca acgttaagcc gggtgatatc ctgcgtgtga aagtcgagca cgccgatgag
    41401 tacgatttgt ggggtagccg ggtttaatta attcaggccg ggcaaaatca tttgtccgcc
    41461 ctgcgaataa tgattactgc cagctaatct gagccctgcc gtttttctta ctcttataca
    41521 gccgctcatc cgccagcatt ttcaagcgtt cgacggtggt acattcattt gaatgcgcta
    41581 caccgatact ggtacttaaa ttaatgcttc ccacctggaa atgaacgatt tcttttagaa
    41641 tcgatagcaa ctcttccagc gttccacccg tataaatcac cgcaaattcg tcaccgccga
    41701 agcggtagat tttaccttta tctaccggga tctgtgattt aaggattttc accactttca
    41761 tcaacacttc atcgcccagc aaatgaccga aggtgtcgtt aatgtttttg aatttatcga
    41821 cgtcaaacag gcacaaataa ccgccttttt ccggcagcgc ttttaaatct tcgtcaaagg
    41881 attttctgcc cattgctttg gtcaggtcgt ccaggaacgc cgagttatac atactgtaga
    41941 gttttttgac atcggcagcc gaaaagaaaa gcccgccatc cagtacgcgt tcaaaatggt
    42001 tattaatgat ggcttctttc tccacaatac cacgattaat cactctgcgt accagataga
    42061 ggcaactggt acccgatatc actgccacca atgcggcaaa gaaaataatc aatgtacaga
    42121 cccgcaaata aacatctttt ttatacgcaa atacgtacaa tccggtcggc aaataggcga
    42181 agtgctccat cgcttccgaa
//
LOCUS       DAKISA010000027        42010 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.027, whole genome shotgun sequence.
ACCESSION   DAKISA010000027 DAKISA010000000
VERSION     DAKISA010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42010
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.027"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            448..600
                     /gene="mokC"
                     /locus_tag="OVW45_001499"
     CDS             448..600
                     /gene="mokC"
                     /locus_tag="OVW45_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCV5525984.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(704..1834)
                     /gene="dnaJ"
                     /locus_tag="OVW45_001500"
     CDS             complement(704..1834)
                     /gene="dnaJ"
                     /locus_tag="OVW45_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCV5525985.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1923..3839)
                     /gene="dnaK"
                     /locus_tag="OVW45_001501"
     CDS             complement(1923..3839)
                     /gene="dnaK"
                     /locus_tag="OVW45_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCV5525986.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4216..4620
                     /locus_tag="OVW45_001502"
     CDS             4216..4620
                     /locus_tag="OVW45_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCV5525987.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4646..5359
                     /gene="msyB"
                     /locus_tag="OVW45_001503"
     CDS             4646..5359
                     /gene="msyB"
                     /locus_tag="OVW45_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCV5525988.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVIQIACLRRMVSATQV"
     gene            5508..6074
                     /gene="satP"
                     /locus_tag="OVW45_001504"
     CDS             5508..6074
                     /gene="satP"
                     /locus_tag="OVW45_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCV5525989.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(6109..6696)
                     /gene="mog"
                     /locus_tag="OVW45_001505"
     CDS             complement(6109..6696)
                     /gene="mog"
                     /locus_tag="OVW45_001505"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCV5525990.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6811..7764)
                     /gene="tal"
                     /locus_tag="OVW45_001506"
     CDS             complement(6811..7764)
                     /gene="tal"
                     /locus_tag="OVW45_001506"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCV5525991.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            8043..9478
                     /locus_tag="OVW45_001507"
                     /pseudo
     CDS             8043..9478
                     /locus_tag="OVW45_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            9548..10324
                     /gene="yaaA"
                     /locus_tag="OVW45_001508"
     CDS             9548..10324
                     /gene="yaaA"
                     /locus_tag="OVW45_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCV5525992.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQHLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10477..10773)
                     /locus_tag="OVW45_001509"
     CDS             complement(10477..10773)
                     /locus_tag="OVW45_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCV5525993.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(10987..12273)
                     /gene="thrC"
                     /locus_tag="OVW45_001510"
     CDS             complement(10987..12273)
                     /gene="thrC"
                     /locus_tag="OVW45_001510"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCV5525994.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(12274..13206)
                     /gene="thrB"
                     /locus_tag="OVW45_001511"
     CDS             complement(12274..13206)
                     /gene="thrB"
                     /locus_tag="OVW45_001511"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCV5525995.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCLELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(13208..15670)
                     /gene="thrA"
                     /locus_tag="OVW45_001512"
     CDS             complement(13208..15670)
                     /gene="thrA"
                     /locus_tag="OVW45_001512"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCV5525996.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEGELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15697..15816)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15752..15817)
                     /gene="thrL"
                     /locus_tag="OVW45_001513"
     CDS             complement(15752..15817)
                     /gene="thrL"
                     /locus_tag="OVW45_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCV5525997.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(16031..16717)
                     /locus_tag="OVW45_001514"
     CDS             complement(16031..16717)
                     /locus_tag="OVW45_001514"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCV5525998.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDVIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(17117..17257)
                     /locus_tag="OVW45_001515"
     CDS             complement(17117..17257)
                     /locus_tag="OVW45_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5525999.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            17353..18069
                     /gene="arcA"
                     /locus_tag="OVW45_001516"
     CDS             17353..18069
                     /gene="arcA"
                     /locus_tag="OVW45_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCV5526000.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(18129..19481)
                     /gene="creD"
                     /locus_tag="OVW45_001517"
     CDS             complement(18129..19481)
                     /gene="creD"
                     /locus_tag="OVW45_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCV5526001.1"
                     /translation="MLKSSLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFL
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSVDSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(19539..20963)
                     /gene="creC"
                     /locus_tag="OVW45_001518"
     CDS             complement(19539..20963)
                     /gene="creC"
                     /locus_tag="OVW45_001518"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCV5526002.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(20963..21652)
                     /gene="creB"
                     /locus_tag="OVW45_001519"
     CDS             complement(20963..21652)
                     /gene="creB"
                     /locus_tag="OVW45_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCV5526003.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(21665..22138)
                     /gene="creA"
                     /locus_tag="OVW45_001520"
     CDS             complement(21665..22138)
                     /gene="creA"
                     /locus_tag="OVW45_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCV5526004.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            22349..23218
                     /gene="robA"
                     /locus_tag="OVW45_001521"
     CDS             22349..23218
                     /gene="robA"
                     /locus_tag="OVW45_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCV5526005.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(23215..23862)
                     /gene="gpmB"
                     /locus_tag="OVW45_001522"
     CDS             complement(23215..23862)
                     /gene="gpmB"
                     /locus_tag="OVW45_001522"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCV5526006.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            23914..24435
                     /gene="yjjX"
                     /locus_tag="OVW45_001523"
     CDS             23914..24435
                     /gene="yjjX"
                     /locus_tag="OVW45_001523"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HCV5526007.1"
                     /translation="MHQVICATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(24520..24846)
                     /gene="trpR"
                     /locus_tag="OVW45_001524"
     CDS             complement(24520..24846)
                     /gene="trpR"
                     /locus_tag="OVW45_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCV5526008.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(24936..26873)
                     /gene="sltY"
                     /locus_tag="OVW45_001525"
     CDS             complement(24936..26873)
                     /gene="sltY"
                     /locus_tag="OVW45_001525"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCV5526009.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQVQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQVDLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            27084..28751
                     /gene="ettA"
                     /locus_tag="OVW45_001526"
     CDS             27084..28751
                     /gene="ettA"
                     /locus_tag="OVW45_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCV5526010.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(29058..30290)
                     /gene="nadR"
                     /locus_tag="OVW45_001527"
     CDS             complement(29058..30290)
                     /gene="nadR"
                     /locus_tag="OVW45_001527"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCV5526011.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(30311..31693)
                     /gene="radA"
                     /locus_tag="OVW45_001528"
     CDS             complement(30311..31693)
                     /gene="radA"
                     /locus_tag="OVW45_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCV5526012.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(31742..32710)
                     /gene="serB"
                     /locus_tag="OVW45_001529"
     CDS             complement(31742..32710)
                     /gene="serB"
                     /locus_tag="OVW45_001529"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCV5526013.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            32816..33460
                     /locus_tag="OVW45_001530"
     CDS             32816..33460
                     /locus_tag="OVW45_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCV5526014.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            33488..34504
                     /gene="lplA"
                     /locus_tag="OVW45_001531"
     CDS             33488..34504
                     /gene="lplA"
                     /locus_tag="OVW45_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HCV5526015.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            34536..34685
                     /locus_tag="OVW45_001532"
     CDS             34536..34685
                     /locus_tag="OVW45_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5526016.1"
                     /translation="MIDLENQEREIINLMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLK"
     gene            complement(34960..35679)
                     /gene="deoD"
                     /locus_tag="OVW45_001533"
     CDS             complement(34960..35679)
                     /gene="deoD"
                     /locus_tag="OVW45_001533"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCV5526017.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(35759..36982)
                     /gene="deoB"
                     /locus_tag="OVW45_001534"
     CDS             complement(35759..36982)
                     /gene="deoB"
                     /locus_tag="OVW45_001534"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCV5526018.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAVGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(37034..38356)
                     /gene="deoA"
                     /locus_tag="OVW45_001535"
     CDS             complement(37034..38356)
                     /gene="deoA"
                     /locus_tag="OVW45_001535"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCV5526019.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(38483..39262)
                     /gene="deoC"
                     /locus_tag="OVW45_001536"
     CDS             complement(38483..39262)
                     /gene="deoC"
                     /locus_tag="OVW45_001536"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCV5526020.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            39520..41070
                     /locus_tag="OVW45_001537"
     CDS             39520..41070
                     /locus_tag="OVW45_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCV5526021.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCKKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            41042..41905
                     /locus_tag="OVW45_001538"
     CDS             41042..41905
                     /locus_tag="OVW45_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCV5526022.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
BASE COUNT         9918 a        11371 c        10558 g        10163 t
ORIGIN      
        1 acacggaaag tcatatcagc gctaacgggt gaccctaagc cctgataatc atagcttaag
       61 gtcaggattt ctgagggatt aattaggaaa agtaataatt aatttcttgt cagtcggcgt
      121 tatttgttag gacaatattt caaaaataat ccagcttgtc gtcaataatt gcgataaata
      181 gataaattaa agttaaataa ttaaatcatt aaaaaattac gccacaaaaa tgcgaggcgt
      241 cttcaggatt agaatcgata aaacagaatg ggttaaagag agcgatataa tagcggcggg
      301 tgcttgaggc tatctgtctc aggcattagc tgaacggcag atagagaaaa gccccgagtg
      361 atattttact atcaacccga ggccccctat atgctgaaca catgtagagt gcctcttact
      421 gaccgtaagg tcaaggagaa gagagcaatg aagcagcata aggcgatgat tgtcgccctg
      481 atcgtcatct gtatcaccgc cgtagtggcg gcgctggtaa cgagaaaaga cctctgtgag
      541 gttcacatcc gaactggcca gacggaggtt gctgttttca cggcttacga atccgagtaa
      601 gagcaacggc ggggagtgat ccccgccact tttcaggtgc tcgcatatct tcaacgcacc
      661 ctaattttac ccaagcctgc ccacgggcag gcttttgggg aggttagcgg gtcaggtcgt
      721 caaaaaactt cttcacacca tcaaagaagc tctttgagcg cgggctgttg tgctcaccgg
      781 ttgggccacc gaagctttct tgcagctctt gcagcaactg tttctgcttc tcgttcaggc
      841 ctaccggtgt ttcgacgaca acgcggcaca gcaaatcacc ctgtgcgcca ccgcggacag
      901 acttgacgcc tttaccgcgc atacggaaaa gcttaccggt ctgggtttcg ccaggcactt
      961 tcagtttgac gcgaccatca agggtcggta cttcgatttc gccacccagc gccgccatag
     1021 cgaagttgat cgggacttcg caatagaggt tgttgccttc acgctcgaaa atcgggtgct
     1081 gtttaacctg aacctgaacg tacagatcgc ctgccggtgc gccatgctcg cccgcttcac
     1141 cttcgcccgc aagacggatg cggtctccag tgtccacccc tgccgggatt ttaacggaca
     1201 gcgttttgct gcgctcaaca cgaccatgac catgacattt gttgcacgga tctttgatca
     1261 gcgtaccgcg gccctgacag tgtggacagg tctgctgtac agcgaagaat ccctggcgca
     1321 tctgcacctg accagaacca tgacaggtcg gacaggtctg cggctgtgta cctggttttg
     1381 caccgctacc gtggcaaacg tcacactctt ccagagtcgg aatgcggatc tctttggtca
     1441 cgccacgtac agcttcttcg agggtgagct ccatgttata gcgtaaatca gcaccgcgcg
     1501 ccgcacgttg acgaccacgt ccgccgccaa aaatatcgcc gaaaacgtca ccaaaaatat
     1561 cgctgaagtc tgcgccgccg ccaaaaccgc cgccgcccat gccaccttgc tcaaacgcag
     1621 catgaccata ctgatcgtat gccgcacgtt tttgcgagtc ggtcagaact tcataagctt
     1681 ccttgatctc tttaaatttc gcctcggcct ctttgtcacc ctggttacgg tccgggtggt
     1741 atttcatggc caggcgtttg taggcctttt tgatttcacg ctcttccgct gttttggaaa
     1801 cgcctaaaat ctcgtaataa tcttgcttag ccatcttttt taaattgccc ctagatgaat
     1861 gcacgggcgg agaggaaatt ccccttcgcc cgtgtcagta taattacccg tttatagggc
     1921 gattattttt tgtctttgac ttcttcaaat tcagcgtcga caacatcgtc atctttcgcg
     1981 ttgtttgcag aagcatcagc accggcagtc tgctgctggg catgttgctg ctgggcgatt
     2041 tccatcagtt tctgggaaac ctgtgccagt tcctgcattt tcgcttcgat agcggctttg
     2101 tcttcacctt tcagagcagt ttccagtgca gtcagcgcag actcgatagc agttttgtcg
     2161 tcagccggca gtttgtcgcc tgcttcttca acctgcttac gggtgctgtg cagcagatgg
     2221 tcgccctggt tgcgagtctg taccagctct tcaaacttac ggtcagcttc ggcgttagct
     2281 tctgcgtcgc gtaccatttt ctggatttca tcttcgttca gaccagaaga agccttgatg
     2341 gtgatcttct gctctttacc gctgttttta tctttcgcgg aaacgtgcaa gataccgtca
     2401 gcatcgatat cgaaggtaac ttcgatctgc ggcatgccgc gcggtgccgg gttgatacca
     2461 tccaggttga actgacccag agatttgtta tcagctgcac gtttacgttc accctgcagc
     2521 acatggatgg ttaccgcaga ctggttgtct tcagcggtag agaacacctg gctgtgcttg
     2581 gtcgggatag tggtattttt cgcgatcagc gtcgtcatca caccgcccat ggtttcgata
     2641 cccagagaca gcggggtaac gtccagcagc agtacgtctt ttacgtcacc agtcagaaca
     2701 ccaccctgaa cagcagcacc gattgctaca gcttcgtccg ggttaacgtc tttacgcggc
     2761 tctttaccaa agaactcagc aactttcttc tgaaccattg gcatacgagt ctgaccaccc
     2821 acgaggataa cgtcgtcgat atcagatacg gacaggccag cgtcctgcag tgcaactttc
     2881 agcggctcaa tggaacggtt taccagatct tcaaccaggc tttccagttt cgcacgagtc
     2941 actttgatgt tcatgtgttt cggaccggtc gcatctgcag tgatgtacgg caggttaacg
     3001 tcggtctgct gagcggaaga cagttcgatt ttcgcttttt ctgccgcttc tttcaggcgc
     3061 tgcattgcca gcggatcgtt gcgcaggtca atgccctgat ctttcttgaa ttcttcaacc
     3121 aggtagttga tcagacggct gtcgaagtct tcacccccca ggtgggtatc accgttggta
     3181 gccagaactt cgaaggtttt ttcgccgtca acttcgtcga tttcgataat agaaatatcg
     3241 aaagtaccac cacccaggtc ataaaccgcg atagtacggt tgccagtacc tttgtccaga
     3301 ccgtaagcca gcgcagctgc ggtcggttcg ttgatgatac gttttacttc cagaccagcg
     3361 atacggcctg cgtctttggt tgcctgacgc tgagcatcgt taaagtatgc cggtacggtg
     3421 ataacagctt cagttaccgg ttcacccaag taatcttcag cggttttctt catttttttc
     3481 agcacttcag cagaaatctg cggcggtgcc attttctggc ctttaacttc gacccatgcg
     3541 tcgccgttat cagcagcaat aattttgaac ggcatgatgg aaacatcacg ctgtacttct
     3601 tcgtcctgga agcggcggcc aatcaggcgt ttaatcgcaa acagggtgtt ttgcgggttc
     3661 gtcactgcct gacgtttagc cggctgacca accagagttt caccatcctg ggtataggca
     3721 ataatagaag gcgtggtgcg atcgccttcg gcgttctcca gcacgcgagg agtggtgcca
     3781 tccataatcg ctacacaaga gttggtagta cccaggtcga taccaattat tttacccatc
     3841 taaacgtctc cactatatat tcggtcatca tgtggttgtg agtctgtaat aggggcgaaa
     3901 cgtctggttt caactgccca atttcatttt tttgcagact cactcactgc ggttgactac
     3961 taaatggggt cgtaacccac gtcatcaagg gggagatgca aaaatttttt gtgcttttgc
     4021 gcagaaattt cgcgatatgg caggacaaga cgtaaaattg acagcaattt tattcatata
     4081 aagaatgaat cgcagccaag gcattcatca aaaaattgta ataaaaagaa aagattacgt
     4141 acctgaatct tctctttatc agcagtaaac tagtgggtat tcatccctct acctcttccc
     4201 actaagagaa tccttatgaa atccgttttt acgatttccg ccagcctggc gattagcctg
     4261 atgctgtgct gcacggcgca ggcaaacgac cataaactcc tcggcgtcat tgcaatgccg
     4321 cgtaacgaaa ccaacgatct ggcgctgaaa cttcctgttt gtcgcattgt gaaacgcata
     4381 caactctctg ccgaccatgg cgatttacag ttaagcggtg catcggttta tttcaaagcc
     4441 gcccgtagcg ccagtcagag tctgaatatt ccttcagaaa taaaagaagg gcaaaccact
     4501 gactggatca acattaacag tgataacgac aataaacgct gcgtctcaaa aatcaccttt
     4561 tccggtcata cggtgaactc atcggatatg gccacgctga aaattatcgg cgacgactaa
     4621 cgccagaata tagaaaccac aaaaaatgaa tgttaattac ctgaatgatt cagatctgga
     4681 ttttcttcag cattgtagtg aggaacagtt ggcaaatttc gcccgattgc tcacccataa
     4741 tgaaaaaggc aaaactcgcc tctccagcgt actgatgcgc aacgaactgt ttaaatcgat
     4801 ggaagggcat cccgagcaac atcgccgcaa ctggcagctg attgctggag aattacagca
     4861 ttttggtggc gatagtatcg ccaacaaact gcgcggacac ggtaaattgt atcgggccat
     4921 tttgctcgat gtttcaaaac gattgaagct gaaagccgac aaagagatgt ctacgtttga
     4981 gattgagcaa cagttactgg aacaatttct gcgtaacacc tggaagaata tggacgagga
     5041 acataagcag gagtttctgc acgcggtcga tgccagggtg aatgagctgg aagagctgct
     5101 gccgctgctg atgaaagaca aattattggc aaaaggcgtg tcgcatttgc tttccagcca
     5161 attgacccgc attttacgca cccacgcagc aatgagcgtg cttgggcatg gtttgctgcg
     5221 cggcgcgggt ctgggaggcc ctgtgggcgc agcattaaat ggggttaaag cggtcagcgg
     5281 cagtgcctat cgcgtgacaa ttccagccgt aatacaaatc gcctgcctgc gccggatggt
     5341 tagcgccact caggtctgac accgtaaacc ggggaaaatt ttcattttcc cctcaaaaga
     5401 tcgtagacac tgccccactg gctgattatt atgccgcgcc ctgaaaacac tactgttatt
     5461 cagggaaatt atttcaccat tcattcgatg atgatttttg aggaattatg ggcaacacta
     5521 agttggctaa tccggcaccg ctgggcctga tgggcttcgg catgaccacc attctgctta
     5581 acctgcacaa cgtgggttat ttcgctctgg acggtattat tcttgccatg ggcattttct
     5641 acggcggcat cgcgcaaatt tttgccggtc tgctggagta caaaaaaggc aacactttcg
     5701 gtttaaccgc attcacctct tacggttctt tctggttgac gctggttgcg attctgctga
     5761 tgccgaaact gggtctgacc gatgcgccaa atgcacagtt ccttggtgtc tacctgggtc
     5821 tgtggggcgt atttacgctg tttatgttct tcggcacgct gaaaggcgca cgcgttctgc
     5881 aattcgtttt ctttagcctg actgtgctgt ttgccctgct ggcgatcggt aacattgccg
     5941 gtaacgccgc aatcatccac tttgccggct ggattggtct gatctgtggt gccagtgcaa
     6001 tctatctggc gatgggtgaa gtactgaacg agcagtttgg tcgcaccgtt ctgccgattg
     6061 gtgaatccca ctaattgttt tgagatcccc ccgctcgggg ggattttttt attcgctaac
     6121 gtcgcgtctt gcactcttcg gtctgaatgc agcaaccact tctggtgccg tttcaacgta
     6181 tggcccttcc agcaactgaa tgcagtacgg tacgctggca aaaataccgt gtaccacaac
     6241 gttacccgca gcgtccttca caccttccag cgtctcttta atagacttcg gctgaccggg
     6301 taagttaagg atcagcgcct gtttgcgaat cacaccaacc tgacgcgaaa ggatcgcagt
     6361 tggtacaaaa tgcaggctta tctggcgcat ctgttcacca aagccaggca tctcgcggtc
     6421 cgctactgcc agcgtcgcat cgggcgttac gtcacgacgc gccgggccag ttccgcccgt
     6481 ggtgagcacc agatggcaac tcatttcatc cactagctca cacaacgttt gctcgatgat
     6541 cgcctgctca tcggggatta agcgggtttc cagttcaaac ggcgtggtta gcgccgatgt
     6601 cagccattct tccagcgcag ggatgccttt atcctgataa acgccgctgg atgcgcgatc
     6661 agagatggaa actaagccaa tacgtaaagt attcatcaag atttttccgt ttaaacagtc
     6721 tcgttaaaca gaatgataca ctgcgaaggg agtgacagac aggcttcaga agaggtagcg
     6781 tgaccgactt cccggtcacg ctaagaatga ttacagcaga tcgccgatca ttttttccag
     6841 tttttcctgg tcaatagcaa acttacggat accttccgcc agtttatcta ctgccattgg
     6901 atcctggttg tgctgccaca ggaactcgga ctcagtgata cgcgccggac gcgctttcac
     6961 ttcgccggtg taagacagtt tacgttcgat agccccttcg ctctcagcca gctctttcag
     7021 cagtgccggt gcgatggtaa gacggtcgca gcctgccagt tccagaattt cgccgatgtt
     7081 acggaagctt gcgcccataa ccacggtttc ataaccgtgc tctttgtagt actggtagat
     7141 ttcagataca gaaaccacgc ccggatcttc tgccggagcg tactctttct tatcagtatt
     7201 cgctttgtac cagtcaagaa tacggccaac aaatggcgag atcaggaaca cgcctgcttc
     7261 cgcacaagca cgagcctgag cgaaggagaa cagcagggtc aggttacagt tgatgccttc
     7321 tttttccagc tgttctgcag cacggatacc ctgccaggta gaagccagtt tgatcagaat
     7381 acgatcgttg ctaataccag catcgttgta gagtttgatc aggcgttttg ctttcgcaat
     7441 tgacgcttcg gtgtcatagg aaagacgcgc atcaacttca gttgagatac ggcccggaac
     7501 cagtttcagg atttccagac caatatttac tgccagtttg tcggtcgcgt ccacgatctg
     7561 ctgcgcgcga tcgttgctct gctgtttcgc ccaggcgaca gcatcatcaa tcaacttacg
     7621 gtattccgga atctgcgctg cgttaagaat gagagaaggg ttggttgtgg catcctgcgg
     7681 ttgatacagc ttcattgccg cgatgtcccc agtgtcggcc actacggtgg tgtactgacg
     7741 aagggaggtc aatttgtccg tcatgatagt atttctcttt aaacagcttg ttagggggat
     7801 gtaaccggtc tgccctgatg atatcacgac gcaacgtcag cgcaaccgcc gacaagacgg
     7861 ttatcgccag gcgagactgt ttcggatttc tgacactgcg ttgatatcgc ccgccatttt
     7921 tatacaaaac ctcatgtatg ctacgcagaa gttatcaagt acctcgtagc gtatatactt
     7981 cttaaacaat tggtaacgtt tacacaggaa agtcatcgcg accggcaata agagggatat
     8041 gcatgccaga ttttttctcc tttattaaca gcgtcctttg gggatcggta atgatttacc
     8101 tgctcttcgg cgcaggttgt tggttcactt ttcgcaccgg atttgtgcag tttcgctaca
     8161 tccgccagtt tggcaaaagt cttaaaaata gcattcatcc acagccaggc ggtttaacct
     8221 catttcagtc attgtgtacc agtcttgcgg cgcgcgtggg tagcggcaac ctggccggcg
     8281 ttgcgctggc tattaccgcc ggtggacctg gagccgtctt ttggatgtgg gttgccgcgt
     8341 ttatcggcat ggcgacctcg tttgccgaat gttcccttgc acaactttat aaagaacgtg
     8401 acgtcaatgg acagtttcgt ggaggaccgg catggtatat ggcgcgcggg ctggggatgc
     8461 gttggatggg cgttctgttc gccgtctttt tgctcatcgc ctacggcata attttcagcg
     8521 gagttcaggc gaacgccgtt gcccgagccc tgagttttct tttgattttc ccccgctggt
     8581 gacaggcatt attctcgctg tctttgctct gctggcaatc actcgcggtc ttcatggcgt
     8641 cgcccggctc atgcaggggt ttgtcccgtt gatggcgata atctgggtgc tgaccagcat
     8701 ggtgatttgc gtaataaata tcgggcaact tccccacgtc atttggtcta tttttgagag
     8761 tgcttttggc tggcaggaag cggcaggcgg cgcggcggga tataccttaa gccaggcgat
     8821 tactaacggt tttcagcgca gtatgttttc caatgaggcg ggaatgggtt cgacgccaaa
     8881 cgcggcagcg gcagcggcag cggcgtcctg gcctccgcat ccggcagcgc aagggattgt
     8941 ccagatgatt ggcattttta tcgacaccct ggtcatctgt acggcaagcg ccatgctgat
     9001 attactggcg ggtaacgaca caacctacat gccgctggaa ggtattcagc ttatccagaa
     9061 ggcgatgcgg gtgctaatgg gttcctgggg tgctgagttt gttaccctcg tggttattct
     9121 gtttgccttc agctccatcg ttgccaacta catttatgcc gaaaacaatc tcttcttttt
     9181 acgcctgaac aaccctaaag cgatctggtg tttgcggatc tgcaccttcg caacggtcat
     9241 cggcggcacc ttgctaagtc ttccgctgat gtggcaactg gcagatatca taatggcctg
     9301 catggctatt accaatttga ccgccatttt actgctctcg cctgtggttc ataccattgc
     9361 cagtgattat ctacgccagc gtaaactcgg cgtgcgcccg gtgtttgatc cgttgcgtta
     9421 tccggatatc ggtcgccagc tttctccgga cgcgtgggat gatgtttcgc aggagtaatc
     9481 acaactatcg atcaactcat tctctttttt tgctaaagtc ggcataaatt tcctgcaagg
     9541 actggatatg ctgattctta tttcacctgc gaaaacgctt gattaccaaa gcccgttgac
     9601 caccacgcgc tatacgctgc cggagctgtt agacaattcc cagcacttga tccatgaagc
     9661 gcggaaactg acgcctccgc agattagcac gctgatgcgc atcagcgaca aactggcggg
     9721 tatcaacgcc gctcgctttc atgactggca gccagatttc acgccggaga atgcccgcca
     9781 ggctattttg gcgtttaaag gtgatgtcta caccggcttg caggccgaaa ctttcagcga
     9841 agacgatttc gattttgccc aacagcattt gcgaatgctt tccggcttgt atggcgtact
     9901 gcgcccgctc gatttaatgc agccttatcg tctggaaatg gggatccgtc ttgagaatgc
     9961 ccgagggaaa gatctgtatc aattctgggg agatatcatc accaacaagc tgaacgaggc
    10021 gctcgcagca caaggcgata atgtggtgat taatctggcg tcagatgagt attttaaatc
    10081 ggtgaagccg aagaaattga atgccgagat tatcaagccg gtgttcctcg atgagaagaa
    10141 cggcaaattt aagataatca gcttctacgc taagaaagca cgcggtctga tgagtcgttt
    10201 cattattgaa aatcggctga ccaaaccaga gcaactgact ggttttaata gcgaaggtta
    10261 cttctttgat gaagcttcct ccagcaatgg cgaactggtg tttaaacgct acgagcagcg
    10321 ttaagtttgc agtcaatgcc ggatgcggcg tgaacgcctt atccggccta caaaagcatg
    10381 cagattcaat atattgcaga attcacgtag gcctgataag acgcgccagc gtcgcatcag
    10441 gcgctgaatg ccggattgtt acccggcatt tgtcatttag cggtgatgtt tgcctggacc
    10501 atgaccgccg tgatgatcat gaggagcttt cttatggtgg cgcggcggtg gcggcggtcc
    10561 gtgtgggtgc cagcgattgc ctcgccattc ataatgttgt ttccaccagc cgtggtcgcg
    10621 ccagtgacct ccatcccagt aatagccacg attatcacga tcgcctatct gtaattttac
    10681 tgacgggact aacgtaattt ccgcagcctg tgctgccatg ggagcgacca gaaccaggga
    10741 aagtgcgagt acgatagatt gcatcttttt cacaggttac tccttttatc attgcccgtt
    10801 atgggtcgat atccggagat tacgcgatga gaacgacggt tgttattctc cgcaatccta
    10861 atctctaagt taccgcattt attgagaatt tctccttttt ccctgtcgtt tttctccata
    10921 atttcttcat aaaaaagccg ggctgcataa aagcaaaccc ggcctgattg agataatgaa
    10981 tagattttac tgatgattca tcatcaattt acgcaacgca gcaaaatcgg cgggcagatt
    11041 atgtgaaagc aagggtaaat cagcacgttc tgccagctct tttggcagat ccaacgtttc
    11101 accgagaatc gcttccacgc tctctttaaa tttcgccgga tgcgcggtgc cgaggaacaa
    11161 gccatattcg cctggattca actggtcacg cagcgcacga tacgctacgg cagcgtgcgg
    11221 ctccgaggtg tagcccagtt cttttaactc acgcattgtc tgttgcgtgg tttcatcatc
    11281 cacggctgca taacccagct ctttcagttg ccagattttg cggcggaaca actcttccac
    11341 acgcggccag ttgttcggct gactcacgtc catcgcgttg gataacgtcg cctgagtcgc
    11401 tttgggtgac cactgaccgt cgtgcaggaa acgtggcacg gtatcgttca cgttggtcgc
    11461 agcaataaaa cgtttcaccg gcagaccgag tgacttcgcc agcagacccg ccgtcaaatc
    11521 gccgaagttt ccgcttggca ccgagacaac cagctggttg cgcgcttcct gcggcagctg
    11581 cgcaactgct tcaaagtagt agcaaatctg cgccagcaaa cggctgatgt taatcgagtt
    11641 agccgagttt aaccccagcg ccactttcag ttcttcatca tcaaacgcct gcttcaccag
    11701 cgcctgacag gcatcgaaat cgccgtcgat ggcaacagtt tcgatattgc cgcccaacgt
    11761 acagaacagt ttttcttgca gtggactgat tttgcctcgt ggatagagga taaccacttt
    11821 cacattcggc aaaccgtaga aagcatgagc cactgccgct ccggtatcac cggaggtcgc
    11881 ggtcagaatg gtcactggct tatcgcccgc aatatgggtc agcatttgtg ccataaagcg
    11941 accgccgaaa tctttaaatg ccagcgttgg cccgtggaac aattccagac aaccgacatc
    12001 gctttcaaca ttggcgaccg gagccgggaa ggcaaacgcc gcgcgcacgc gctcttccag
    12061 gatttcctgc gggatttcat caccaataaa cgccgagagg atcttcgcac tgcgggtgac
    12121 aaaatccagc ttcagcatct catcaatttc agtcaggctg aattccggca ggtcatgcgg
    12181 aaaaaacagc ccctgatttt tgcccaaccc ctgggttacg gcttgcgcaa agctgacctg
    12241 ctcgttgtga tctttcagat tgtagagttt catttagttt tccagtactc gtgcgcccgc
    12301 cgtatccagc cggcaaatat gaacaaaacc ttcctgattt tgcaggtagt tcttacccaa
    12361 ccagtcggca acgcgctggg cggtatccgg cttgtcacat agagcgaaca aggtcgggcc
    12421 ggagccggag ataccgctcg ctaccgcgcc gatttccgcg accgcctgcc gcgcctgccg
    12481 gaagccaggc agtaaccgtt cacggtaggg ttcagcgata acatctttca tcagcttcgc
    12541 ggcaagctca ggctgacggg aatagcaggc gtgaatgaag ccagccagat gtcgcccgtg
    12601 cgcaatgcaa tcctggcggc gatactgcgc cggtaaaata gcccgggctt ctgccgtcga
    12661 gactttaatt cccggatacg ccagcaccca cagccactca tcaaaccctg gcacttgctg
    12721 gctgatgatg tcgttttctt cgatcatcaa ctgcatacca ccaagaaaac acggtgccac
    12781 gttgtcgtaa tgaatgctgc cggagatacg tccttccagc tcgcccatca aagccagcaa
    12841 acgggtgtca ttaagtggct tgccgcagtg ttcattcatc gccatcagcg ccgcgaccac
    12901 cgaacaggcg ctggagccta agcccgagcc gatcggcata ttcttttcca gagtcatcgc
    12961 cactggaatt tgcttgccca gctccaggca aaaacgctcc cagcactgat aaacgatatt
    13021 ttcccgtggt tctgacggca gcttatcggc aaagcgtccg aggttgttga gactgaatgt
    13081 ctctgccgcc tcaaccgtga ctacatctcc gagcaatgca ccatcaacgg gtgtcaccgc
    13141 cgccccgagc acatcaaacc cgacgctcat attggcactg gaagccgggg cataaacttt
    13201 aaccatgtca gactcctaac ttccatgaga gggtacgtag cagatcggca aagacaccag
    13261 cagcggtaac gtcattgccc gcaccatatc cgcgcagcac caacggcagc ggctgataat
    13321 agtggctata aaaggccagg gcgttttcgc catttttcac tttgaacagc ggatcattcc
    13381 catccacttc ggcaatcttc acgcggcaga caccatcttc atcaatattg ccaacatagc
    13441 gcaaaacttt tccttcatca cgggccttcg ccacgcgcgc ggcaaagaga tcgtcgagct
    13501 gtgacagatt cgccataaaa gcggcaacat caccctcggc gttaaactct gcgggcagca
    13561 caggttcaat ttcaatatcc gccagctcca gttcacgtcc cgtttcacga gcgagaatca
    13621 atagtttacg cgccacatcc ataccagaaa gatcatctcg cgggtccggt tcggtataac
    13681 ccatttcccg cgccagcgtg gtcgcctcgg agaaactcat gccttcgtct aacttgccga
    13741 agatataaga aagcgaacct gaaagaatgc cggagaactt caccaattca tcacctgcat
    13801 tgagcagatt ttgcaggttc tcaataaccg gtaatccagc cccaacgttg gtgtcataga
    13861 ggaatttacg ccgcgatttt tccgccgcat aacgcaactg atgatagtaa tccatcgacg
    13921 aggtgttggc ctttttgttc ggcgtgacaa cgtggaaacc ttcgcgcagg aagtcggcat
    13981 attgatccgc cactgcctgg ctggaggtgc agtcaacaat gaccgggttc agcagatgat
    14041 attctttcac gaggcgaatt aagcgcccga gattaaacgg ctctttggct tgtgcaagtt
    14101 cttcctgcca gttttccaga ttaaggccat gcacactagt gagcagagcc ttcgagttgg
    14161 caacgccgca gacacgtaag tcgatatgtt tattcttcag ccagctttgc tgacgcttca
    14221 gttgctccag cagcgcaccg ccaacgccac cgacgccaat cacaaacact tcgataacct
    14281 gatcggtatt gaacagcatc tgatgagtaa cgcgcacgcc agtggtcgca tcatcgttat
    14341 ttaccacgac agagattgag cgttcagaag atccctgagc aatggcgaca atgttgatat
    14401 tggcgcgggc cagcgcggca aagaatttcg ccgagatccc acgcaaggtg cgcataccat
    14461 cacctaccac cgagataatg gccagccgtt ccgtcaccgc cagcggctcc agtaagcctt
    14521 ctttcagttc caggtagaac tcttcctgca ttgcccgttc agcccgcaca cagtcgcttt
    14581 gcggaacgca gaaactgata ctgtattcgg aagatgattg cgtaatcagc accacggaaa
    14641 tacgggcgcg tgacatcgcg gcaaagacgc gcgccgccat gccgaccatt cctttcatcc
    14701 ccgggccgga aacgctgaac attgccatgt tattcagatt ggaaatgccc ttgaccggta
    14761 attcgccttc atcacgactg gcaccaatga gcgtacctgg tgcttgagga tttccggtat
    14821 ttttaatcag gcaagggatc tggaactggg cgatgggggt aatggtgcgg gggtgaagaa
    14881 ctttagcgcc gaagtaggaa agctccatcg cttcctggta ggacatcgac ttcaacaacc
    14941 tcgcatcggg cacctgacgc gggtcgcagg tatagacccc gtcaacgtcc gtccaaatct
    15001 cgcaacaatc ggcgcgtaaa caggcagcca gcaccgccgc ggagtagtcg gaaccgttgc
    15061 gtccaagtac caccagttcg cctttttcat taccggcggt gaagcctgcc atcagcacca
    15121 tgtgatcagc cggaatacga cttgccgcaa tacggcgggt ggactcagca atatcgacgg
    15181 tagattcgag gtaatgcccc actgccagta gtttttcgac cggatcgata acggtaacgt
    15241 tgtgaccacg cgcttctaac acgccggcca taatggcgat cgacattttc tcgccacggc
    15301 aaatcagcgc agcgttaatg ctatccgggc actgccctaa caaactaatg ccatgcagga
    15361 catgttttat ttgggcaaat tcctggtcga cgaaagtttt caattgcgcc agcgggaatc
    15421 ccggctgggc ggcggcgagt cccgtcaaaa gttcggcaaa aatacgttcg gcatcgctga
    15481 tattgggtaa agcatcctgg ccgctaatgg ttttttcaat catcgccacc aggtggttgg
    15541 tgattttggc gggggcagag aggacggtgg ccacctgccc ctgcctggca ttgctttcca
    15601 gaatatcggc aacccgcaga aaacgttctg catttgccac tgatgtaccg ccgaacttca
    15661 acactcgcat ggttgttacc tcgttacctt tggtcgaaaa aaaaagcccg cactgtcagg
    15721 tgcgggcttt tttctgtgtt tcctgtacgc gtcagcccgc accgttacct gtggtaatgg
    15781 tgatggtggt ggtaatggtg gtgctaatgc gtttcatgga tgttgtgtac tctgtaattt
    15841 ttatctgtct gtgcgctatg cctatattgg ttaaagtatt tagtgaccta agtcaataaa
    15901 attttaattt actcatggca ggtaaccagt tcagaagctg ctatcagaca ctcttttttt
    15961 aatccacaca gagacatatt gcccgttgca gtcagaatga aaagctgaaa aatacttact
    16021 aaggcgtttt ttatttggtg atattttttt caatatcatg cagcaaacgg tgcaacattg
    16081 ccgtgtctcg ttgctctaaa agccccaggc gttgctgtaa ccagtcgacc agttttatgt
    16141 catctgccac tgccagagtc gtcagcaatg ccatgactcg ttcgcgtaaa gcttgcagtt
    16201 gatgttggtc tgccgttgtg tcacttttcg ccggttgttg tattaatgtt gctaattgat
    16261 agcaatagac catcaccgcc tgccccagat tgagcgaagg ataatccgcc accatcggca
    16321 caccagtaag aacgtcagcc aacgctaact cttcgttagt cagcccggaa tcttcgcgac
    16381 caaacaccag cgcggcatgg ctcatccatg aagatttttc ctctaacagc ggcaccagtt
    16441 ctactggcgt ggcgtagtaa tgatatttcg cccgactgcg cgcagtggtg gcgacagtga
    16501 aatcgacatc gtgtaacgat tcagccagtg ttgggaaaac tttaatatta tcaataacat
    16561 caccagatcc atgtgcgacc cagcgggtgg ctggctccag gtgtgcctga ctatcgacaa
    16621 tccgcagttc gctaaaaccc atcgttttca ttgcccgcgc cgctgcccca atattttctg
    16681 ctctggcggg tgcgaccagg ataatcgtta tacgcatatt gccactcttc ttgaccaaat
    16741 aaccgcgaac agggtgatca ctgtcaactt attacgcggt gcgaatttac aaattcttaa
    16801 cgtaagtcgc agaaaaagcc ctttacttag cttaaaaaag gctaaactat ttcctgactg
    16861 tactaacggt ttaattgtta aaaaatgcta catatccttc tgtttactta ggataatttt
    16921 ataaaaaata aatctcgaca attggattca ccacgtttat tagttgtatg atgcaactag
    16981 ttggattatt aaaataatgt gacgaaagct agcatttaga tacgatgatt tcatcaaact
    17041 gttaacgtgc tacaattgaa cttgatatat gtcaacgaag cgtagtttta ttgggtgtcc
    17101 ggcccctctt agcctgttat gttgctgtta aaatggttag gatgacagcc gtttttgaca
    17161 ctgtcgggtc ctgagggaaa gtacccacga ccaagctaat gatgttgttg acgttgatgg
    17221 aaagtgcatc aagaacgcaa ttacgtactt tagtcatgtt acgccgatca tgttaatttg
    17281 cagcatgcat caggcaggtc agggactttt gtacttcctg tttcgattta gttggcaatt
    17341 taggtagcaa acatgcagac cccgcacatt cttatcgttg aagacgagtt ggtaacacgc
    17401 aacacgttga aaagtatttt cgaagcggaa ggctatgatg ttttcgaagc gacagatggc
    17461 gcggaaatgc atcagatcct ctctgaatat gacatcaacc tggtgatcat ggatatcaat
    17521 ctgccgggta agaacggtct tctgttagcg cgtgaactgc gcgagcaggc gaatgttgcg
    17581 ttgatgttcc tgactggccg tgacaacgaa gtcgataaaa ttctcggcct cgaaatcggt
    17641 gcagatgact acatcaccaa accgttcaac ccgcgtgaac tgacgattcg tgcacgcaac
    17701 ctgctgtccc gtaccatgaa tctgggtact gtcagcgaag aacgtcgtag cgttgaaagc
    17761 tacaagttca atggttggga actggacatc aacagccgtt cgttgatcgg ccctgatggc
    17821 gagcagtaca agctgccgcg cagcgagttc cgcgccatgc ttcacttctg tgaaaaccca
    17881 ggcaaaattc agtcccgtgc tgaactgctg aagaaaatga ccggccgtga gctgaaacca
    17941 cacgaccgta ctgtagacgt gacgatccgc cgtattcgta aacatttcga atctacgccg
    18001 gatacgccgg aaatcatcgc caccatccac ggtgaaggtt atcgcttctg tggtgatctg
    18061 gaagattaat cggctttacc accgtcaaaa aaaacggcgc tttttagcgc cgtttttatt
    18121 tttcaacctt atttccagat acgtaattca tcgtccgttg taacttcttt actggctttc
    18181 attttcggca gtgaaaacgc ataccagtcg atattacgag tcacaaacat catgccggcc
    18241 agcgccacca ccagcacact ggttcccaac aacagcgcgc tatcgacaga gttgagcagt
    18301 ccccacatca ctccatccag caacaacagc gcgagggtaa acaacatgct attgcgccag
    18361 cctttcaata ccgcctgcaa ataaataccg ttcattaacg ccccaatcag actggcgatt
    18421 atccatgcca cggtaaaacc gatatgttca gaaagcgcca gcaagagcaa ataaaacatc
    18481 accaatgaaa gccccaccag caaatattgc attgggtgta aacgttgcgc ggtgagcgtt
    18541 tcaaaaacaa agaacgccat aaaagtcagt gcaatcagca gaatggcgta cttagtcgcc
    18601 cggtccgtta attggtattg atcggctggt gtcgtcactg cgacgctaaa cgcgaggaag
    18661 ttttcccagc cggtatcatt gcctgaagca aaacgctcac cgagattatt agcaaaccag
    18721 ctgctttgcc attgcgcctg aaaacctgac tcgctaactt cccgtttggc tggcaaaaaa
    18781 tcacctaaaa aactgggatg cggccagttg ctggttaagg tcatttcgct attacgcccg
    18841 gcaggcacca cagaaagatc gccggtaccg cttaaattca gggccatatt cagcttcagg
    18901 ttctgcttcc gccagtcccc ttcgggtaaa gggatatgca cgccctgccc gccttgctct
    18961 aacccggtgc cgggttcaat ggtcagcgcc gttccgttaa cttcaggcgc tttcaccaca
    19021 ccaataccac gcgcatcccc gacgctaatc acaataaatg gcttgccaaa ggtgatattt
    19081 ggcgcgtcga gttcgctaag acgcgaaaca tcgaaatcgg cttttagcgt taaatcactg
    19141 tgccagacct gaccggtata aatccctatc ttgcgttctt ccacgttctg attgccatca
    19201 accatcaatg actcaggtaa ccaaaaatgg ataaaacttc gtttccgctc cacggtttta
    19261 tcctcttcct gtaccgtata aagctcggtc acaggaatag cgataagcgg cccaacaagt
    19321 ttttgcggcc cgctggtact ttggcgaatc gcatcttcca catcgctacg gtaatcggca
    19381 cgttcgacaa tcacctgccg aatcagcatt atcggaatca acaacagcaa tactgcacca
    19441 aacagggtgg tcattttcca gaacagggag gatttcaaca tagtcttctc ctttgcaatg
    19501 gcagcaggat acgaaggcta tgtgggaaga atttgaagct atgtgaagtg acggtgaagt
    19561 cgaagcgagg ccagcacgcc accttcctgc acgttgcgca gcgtgacttc gccgttaaac
    19621 aaacgggcga cctcactgac gaacgccaac cccagaccgc tgcttttttg cccatttgca
    19681 cgcggcaaag agtaaaagcg ttcaaaaata cgtgacagcg cgtagtcagg aataccacta
    19741 ccggtatcca gcaccttaag cgtgacgtgt tcctgatcca cttcggcgct tagcgttata
    19801 cgaccgctct cgggggtaaa atcgatggcg ttatccagta aattccccag cgcctgatcc
    19861 agtaacgccg gttcagcagc aacattaacc tcggtgggcg taacatgtag agtgatgttt
    19921 ttttctgcca actgcacggt gcgcgcttcg ctgacgcggc ggaataatgc cgccacatca
    19981 acagcagtca aaacgacttc ctgacgattc tccagtcttg cctggcgtag taacgtttcc
    20041 accagtgcct gcattcgcgc attttgcgtc agaatgttgt cagtaaaacg agccactact
    20101 tcaggcggcg gaccttcgcg taaaatttcc gccgcgccac gaatcgccgc cagtgggctt
    20161 tttagctcat gggttaacgc ataaacatac tgctcaatat agtttttccc ttccagcttc
    20221 acgcgcatac tttccagcgc ctgcgcgagt ttacgcagct cgctactacc gagatcggga
    20281 agaggaacgg gcttattgtc agtgacggaa tcagcatagc gagtgagccg ggcaatggag
    20341 cggttgatcc accaaaccat gcctgcgcca atcaccagtg cgatccccaa caaaatggcg
    20401 ctggcccata aaattcgccg ctcgctgcgt ttaatgaccg gagccatcgc cgcgttcggt
    20461 ttgcctacgc tcaaaacgcc aataagccgc gagccgtcca taatcggcgc ggcaacatac
    20521 atcaccgaac tttcgggatc ggcaggattt tgcaacgtgc tacgcgcacc atactgacca
    20581 cgcaacgtta gccagacgtc attccagcgc gaataatcct gcccaacggc tttatttgcc
    20641 gaatcgaaca atactttgcc ctgcgcatcg gtcatataga catgatattc attgcgcact
    20701 ttgttaatgc caccgatatt ggcgcgaaac gggcgatgtt gtagctgatt aaacgcctgc
    20761 gccagttgcc catgcgttgg gtccccagag agcaaatcgg gacgcgccag ctccgccagc
    20821 aacgttgcgg tatcgattaa cgtcccctcc gttgctcttc gcacgcccgg tttaacttct
    20881 ttgacaaaaa tagccagtac gaaccaggcc gccaccgcca ccagtaaaaa atagcccagc
    20941 agcaaccgca tgccgatacg cattacaggc ccctcaggct atatcccatg ccgcgatgag
    21001 tattaatcgg agaaagatcg gggttgatgg cgcgcagctt ggcacgcaac gttttaatgt
    21061 gggtatcgac ggtgcgatcg taggtgtcct gcgcatcttc ccacacgcta tccattagtt
    21121 gctggcggga ccagacgcgg cctggcgact tgagtaacgt cttcagcaat aaaaactcat
    21181 accgcgtcag cgttaatggc gtgtcaaacc agctgatctg cgccgcgggt tcattcaatt
    21241 caaaatgacc aatacggatg acgggagacg gcgacgagaa cttcttcacc cgacgcagta
    21301 aggtgcgcac cctggcgcac acttcgcggg gtgaaaacgg tttagccaca tagtcgtcag
    21361 cgccaatttc cagcccaagc aggcgatcga cctcttcact tcgggccgtc aggaacagta
    21421 caggtaacgc cggatggagc gccagtaact ggcggcacaa ttcaaagccg ctgatatcag
    21481 gcaaaccaac atcgagaatc atgacgtcgg gaacctgctg gcgagcttta tccagcaccg
    21541 gcaagcctcg ctcaaagacc tcgacggcaa aaccttcctg ctgcaacatg tagaccagcg
    21601 tgtcggctat cccttgctca tcttccacta accagaccgt ttcccgttgc ataaatcgcc
    21661 tctgttattg ccgccacggc atgacaggaa ccgcgctaat cgcgtttttc ggcgaacctt
    21721 ctacaacttt gtcggagtaa gccagataag cgagcgcgtt gcgtttggcg tcataaaagc
    21781 gcacgacctg taacgactta aagaccaggg acgtgcgttt tttgaatact acctcgccct
    21841 gagctttgcc gtttttaata cgatccgaca gttcaatcgg cccgacttgc tgacaagaaa
    21901 tagccgcatc ggaggtatct tccgccagac ccaatccccc tttaatacca cctgttttcg
    21961 cccggctcac ataacaggtg acatttttca catcgggatc gtcaaaggct tccacaacaa
    22021 ttttgtgatc cgggccgatc attttaaata cggtgtcgac cgaaccaatc tcttctgcat
    22081 gagccaatgg ccccagcatt atcaggctta aagacaggat caaatgcttg tatttcatat
    22141 tgttaccatt tttatctatt acacgagcaa ttagttcgtc acggtaaata acatattgat
    22201 taagaacaaa atctcaatac ttttatttcc gtcaagccct aaaacatact ctactaagaa
    22261 aaaaacactg aatgctaaaa cagcaaaaaa tgctattatc caattacctg atgtcaggtg
    22321 ctcgttgttg aaaggatgag gatattttat ggatcaggcc ggcattattc gcgacctttt
    22381 aatctggctg gaaggtcatc tggatcagcc cctgtcgctc gacaatgtag cggcgaaagc
    22441 aggttattcc aagtggcact tacagagaat gtttaaagat gtcactggcc atgctattgg
    22501 cgcgtatatt cgtgctcgtc gtttgtcgaa atcggcggtc gcactacgcc tgactgcgcg
    22561 tccgattctg gacatcgcgc tgcaataccg cttcgactct caacagacat ttacccgcgc
    22621 attcaagaag cagtttgccc agactcctgc actttaccgc cgttctcctg aatggagcgc
    22681 ctttggtatt cgcccgccgc tacgtctggg tgaattcaca atgccagagc acaaatttgt
    22741 caccctggaa gatacgccgc tgattggtgt tacccagagc tactcctgtt cgctggagca
    22801 aatctctgat ttccgccatg aaatgcgtta tcagttctgg cacgattttc tcggcaacgc
    22861 gccgaccatt ccgccagtgc tctacggcct gaatgaaacg cgtccgagtc aggataaaga
    22921 cgacgaacaa gaggtattct ataccaccgc gttagcccag gatcaggcag atggctatgt
    22981 actgacgggg catccggtga tgctgcaggg cggcgaatat gtgatgttta cctatgaagg
    23041 tctgggaacc ggcgtgcagg agtttatcct gacggtatac ggaacgtgca tgccaatgct
    23101 caacctgacg cgccgtaaag gtcaggatat tgagcgatac tacccggcag aagacgccaa
    23161 agcgggagat cgcccaatta atctgcgctg tgaactgctg attcctatcc gccgttaacg
    23221 ctgcagctca tctaatgcag gggcgtccag atgcgagatg tcccctgcgg tttcaacgac
    23281 ccagccagac gccagccaca ggctttcctg ataatccacg cgcgaaatag aacagttacg
    23341 cagacgtaag cggcgctctg cccatgctgg taatccgaga atcgtactca ccagacatcc
    23401 cagtgcaata ccatggctca ccagcagcgg tcggcttccc tgcggtaagt cccggcaaga
    23461 ttccagtgcc gcattgacgc gatcgctgag ctcctgcatt gactcgcctt caggaatacg
    23521 cccgtcaacg gtgccattga ccagctgccg acgccagttc tcttcttctt cggtcagaga
    23581 atcgatatgt cttttttcca gcacacccat gtttaattca cgcaggcgag aatcaaagat
    23641 gatgtcacag ccgcaggcct gggcgatgat ttccgccgta cgccgggtgc gtcctaaatc
    23701 gctactgata atatgcgtaa tgccaagctc tttggcacgg gttgccacct gcatcgcctg
    23761 ttgctcacct ttggcggtca gcgggctgtc agactggccc tgaatacgtc gctcggcgtt
    23821 ccactgcgtt tcaccgtggc ggactaggta tacctgtaac atgctttttt tccgtatact
    23881 gctgtaacgt taattttctg agtaatgctg attatgcacc aagttatctg tgcgaccacc
    23941 aatcccgcta aaattcaggc cattctgcag gcatttcacg agatcttcgg cgaaggatcc
    24001 tgccatattg catccgttgc cgtcgagagc ggtgtaccgg aacagccctt tggcagtgag
    24061 gaaacgcgcg ctggcgcacg aaatcgggta gcaaatgccc gccgtttact tccagaggct
    24121 gatttttggg tggcgattga agctggcatc gatggtgaca gcactttcag ttgggtggtg
    24181 attgaaaaca ccagccagcg cggcgaggcg cgttcggcta ctttgccgtt acctgcggtg
    24241 attctgcaga aagtgcgtga aggtgaggcg cttggtcccg tgatgtcgcg ttataccggt
    24301 attgatgaga ttggtcgtaa ggaaggggcg atcggcgtgt ttaccgccgg gaaactcact
    24361 cgcgccagcg tctatcacca ggcggtgatc ctggcgctga gtccgtttca caatgccgtg
    24421 tatcaggcat tgtaggacgg ataaggcgtt cacgccgcat ccggcattcg gtgcacgatg
    24481 cctgatgcga cgctgccgcg tcttatcagg cctacaaaat caatcgcttt tcagcaacac
    24541 ctcttccagc cactggcgca gctccacggg cgcggctttc aggctgttag atccacgcgt
    24601 aatcgtcgcg atgcccgcgc cgagttcatt ttttaactca cgctgactca tttcgccacg
    24661 caacagctct tcgacaatac gcacgcgagt ccccaacgct tcgcgctcat ctggcgtcag
    24721 catcaggttt aacaacggta aatggagatc gttttggtag gcattcttaa gcaggtcgac
    24781 aaaacgtaac cactcctggt gacgctgttc tgccatcgct gctgaatagg gtgattgttg
    24841 ggccataata tgtcgccatt gttagcgggg gaagcaaaat gcctcccccg gttgtactcg
    24901 ctaaagagta cgatagcata tcataaacgt gcggatcagt aacgacgtcc ccattccgtg
    24961 gcgctcatca acgtcggttt atcccccatg aaataacggt agtaagcgtc ataagccagc
    25021 acgttcttca cataaccgcg cgtctcggag aacggaatac tctcgacaaa tgccacagcg
    25081 tcgatacgcc cggcgctgtt gccaagccag gttcgcaccc gtccaggtcc ggcgttataa
    25141 gctgctgagg agaaaatacg gttattgcca aactgctgat aaacatattg caggtaactg
    25201 gtgccaatgt tgatattcgt ttccggatcc agcaattgcc cagggctgct ataaccagga
    25261 atagagaaca tcttcaccgt atgggtcgct gtaccaggca taatctgcat caggccgctg
    25321 gcccctaccg gtgatttcac tttcggattc caggcgctct cctgacgggc aatcgccatc
    25381 gcatagcttt gcgggatctc tttcccactg gtgtaacgtt tgaaaagatc gttgtaagcc
    25441 agcgggaatc gctcttccag atgatcccaa agcttcccgg cgatcgttgc ctgaacgcta
    25501 agatcccacc attggttatt gaaagcatac cgagccagtt gagcctgctc tgtttttgac
    25561 ttgctcttca ccagattggc ccactcgcta cgcgcggtgt tatcgagatt ccagtacatc
    25621 aactcgcgca cgcgcgccat ctccggcccc tgagtcaggg cgctgtcaac attctgcggc
    25681 gctttatcaa tcttcagctc atactcttct ccgatgcgtt gtgctgcaac catcgggtag
    25741 aaaccacgct gttgcatgag ttgatgcaaa atctctttcg cttcagcttc acgtccgcgt
    25801 tccagcaata aatccacctg ccagtaacgc cattcatctt tctcttttgc ttccatcggc
    25861 agacgcgcca gccaggtatt caggccgcga cgatcgccgg tgccaagtgc cattcgcaca
    25921 cggcgttcaa tcaacgacgt ggattgcgag cgcataatgg cgtcatcgcg ccatttcgcc
    25981 tgctcgtcgg tgacatcgtt gcccatcaaa cgccaggcga cgatatcgcg cagctcctga
    26041 atctgatctt cattaagctg ctgcacctgg gcaagcgacg ggatcatcag ccgtgcattt
    26101 tcagcatcct gccgcgccac actggcaaac gccaccgctg ccatttgacg ggtaaaatcg
    26161 gtcgctccgg ttgtacgcgc gaaggtcagt accgtattag ggttgttcgc cagtgaaatg
    26221 attgccgagg cgatagtctg gtaatcggca ggcatctgcc ctgccagcac tgttaccagg
    26281 ccagtgttac ccgctttcat cgccagacgg atacgctcta aatacgccag cggatcttgt
    26341 ttacctgacg cacgccagac gctaaataac ttgtcacagg cgttaggctg gctcttgccg
    26401 gttagccaca gctctttcgc cccttgccag gcttcttcac tctgcccggt gttccatttc
    26461 gcatagtagt aattacattg cgcttcggta gttccgggct tttccgggct aaaggctaac
    26521 aagccacgcc agtcttcacg ccgcgccagt tcattgacga aacgagattg cagcgtgcga
    26581 gcgggaggaa gcgtggggtt agcgcgaaca aagttagtga ccgtcaccgc cggttgattc
    26641 atcagatcat cggtgatctg gcggtattcc aggtagggat aaagcggata atccttcagt
    26701 ccaggcatca tttgttcgac cacatccatt tgtcgattat cccaggcctg cttgatttgc
    26761 gcgtaacggc tacgctgctc atccagtgag tcggctcgcg ccacgctgct gaccgtcagc
    26821 agacagacac cggcagccaa cagccgccag gtaacttgtt tggctttttc cacaagcgca
    26881 tcctctaagt gtaaatacat caatgcagcg tcatgccgcg taatagattt atgctaacca
    26941 gtcattgccg tttacgccac gttacggaca cttttttact tttactgcga aggtgatcga
    27001 ccaactggga ttaggcgggg aaaaaggcta cacttcgcct ttgaaaactc atcatcacga
    27061 taaacataga ggcgaagtcc aacgtggctc aattcgttta taccatgcat cgtgtcggca
    27121 aagttgttcc gccgaaacgt catattttga aaaacatctc tctgagtttc ttccctgggg
    27181 caaaaattgg tgtcctgggt ctgaacggcg cgggtaagtc taccctgctg cgcattatgg
    27241 cgggcattga taaagacatc gaaggtgaag cgcgtccgca gccagacatc aagattggtt
    27301 acctgccgca ggaaccgcag ctgaacccgg aacacaccgt gcgtgagtcc attgaagaag
    27361 cggtttctga agtggttaac gccctgaaac gcctggatga agtgtatgcg ctgtacgccg
    27421 atccggatgc cgattttgac aagctggccg ctgaacaagg ccgtctggaa gagatcattc
    27481 aggctcacga cggtcataac ctgaacgtac agctggagcg tgcggcggat gcgctacgtc
    27541 tgccggactg ggacgcgaaa atcgctaacc tctccggtgg tgagcgtcgt cgcgtagcgt
    27601 tgtgccgcct gctgctggaa aaaccagaca tgctgctgct cgacgaaccg accaaccacc
    27661 tggatgccga atccgtggcc tggctggaac gcttcctgca cgacttcgag ggcaccgtgg
    27721 tggcgattac ccacgaccgt tacttcctcg ataacgttgc aggctggatc ctcgaacttg
    27781 accgcggtga aggtattccg tgggaaggca actactcctc ctggctggag cagaaagatc
    27841 agcgcctggc gcaggaagct tcacaagaag cggcgcgtcg taagtcgatc gagaaagagc
    27901 tggagtgggt acgtcaggga actaaaggcc gccagtcgaa aggtaaagca cgtctggcac
    27961 gctttgaaga gctgaacagc accgaatatc agaaacgtaa cgaaaccaac gaactgttta
    28021 ttccacctgg accgcgtctg ggcgataaag tgctggaagt cagcaacctg cgtaaatcct
    28081 acggtgatcg cctgctgatt gatgacctga gcttctcgat cccgaaaggg gcaatcgtcg
    28141 ggatcatcgg tccgaacggc gcgggtaaat cgaccctgtt ccgtatgatc tctggtcagg
    28201 aacagccgga cagcggcacc atcactttag gtgaaacggt gaaactggcg tcggttgatc
    28261 agttccgtga ctcaatggat aacagcaaaa ccgtttggga agaagtttcc ggcgggctgg
    28321 atattatgaa gatcggcaac accgagatgc caagccgcgc ctacgttggc cgctttaact
    28381 ttaaaggggt tgatcagggt aaacgcgttg gtgaactttc cggtggtgag cgcggtcgtc
    28441 tgcatctggc gaagctgctg caggttggcg gcaacatgct gctgctcgac gaaccaacca
    28501 acgacctgga tatcgaaacc ctgcgcgcgc tggaaaacgc cctgctggag ttcccgggct
    28561 gtgcgatggt tatctcgcac gaccgttggt tcctcgaccg tatcgccacg cacatcctgg
    28621 actaccagga tgaaggtaaa gttgagttct tcgaaggtaa ctttactgag tacgaagagt
    28681 acaagaaacg cacgctgggc gcagacgcac tggagccgaa gcgtatcaag tacaagcgta
    28741 ttgcgaagta atgcgtaaaa tgccggatgc ggcgtgaacg ccttatccgg cctacaaacc
    28801 aagaaatttc aatacgttgt gaatctccag gcctgataag acgcggtaag cgtcgcatca
    28861 ggcattgagc accgaatgcc ggatacggcg tgaacgcctt ttccggccta caacagcctt
    28921 agtatccgca atttcgtagc gggtcacccg tgcaggtttt ttcaaacctt gtttaatctc
    28981 gacagcctct tccagagagg ttttcaactc atcaaaaaac ttattttata cctcgccttt
    29041 gagccgtttc atcgcggtta tctctgctcc cccatcatct cccgcaccag ctccacgcag
    29101 cgcaggaaac ggctgtcata atcgtcctct tcaacccgca cgaattcgat attgttctct
    29161 tccagcatct ccaccagcaa gttctggaac tctttgcgat ccaccgaact gccgaggctg
    29221 cgtaaaccat ccgccaccca cggcgtgttg ttctccagca ggatcaccag atcgaaacgg
    29281 tattcatcaa tcaacgcctg tacgaacgga tgctcacgcc cttcgtactt tttgcagaac
    29341 gcctgggtag tgacaaaatc agtgtcaata aacgccactt tattggcata tttcactgca
    29401 aaatcaatgt attgtgcgtg gcccagcgcg attttatcgt aatcggaata ctgcaatgcg
    29461 atctcatcac cgccgaggtg tgaaaagaca taatcgcgac catattccca cgcactggtg
    29521 gtattgaaga tattggcaag tttgtttacc agggtggatt taccgctcga ctcgccacca
    29581 aggatcgcca cggtacgtac aaagaacggc ttcacttccg taggaatata ttcccagtag
    29641 cggaacgggt tttcgcggat ctgcgcaccg ctgatactca taaaggtacg tttcggatcg
    29701 accagcaccg tatcgatccc cagatgttcc atatactgtg gcgcatcggc ttcttccgag
    29761 gtgtagatca ggtccggctg aatccccttt tcagccataa actttttgat gccgttgctc
    29821 cacacatccc agccgtgcgg atacggctcc atgccctctt cgttgaaagc atgaatgcga
    29881 atatttttct gatatttaaa agtttgtaat aaccagcgca gacgatccgg caccgtgggc
    29941 tgctgcgaca tggcgctgtc ttcaaacaac gcgcggtcgc gggtatcgtc aaaacccata
    30001 atgatatgca gctcgtcaac ctggctacag gcgcgctgga taaggtagat atgtccggta
    30061 tgcagcgggt agaacttacc gaatacgaca ccaatggttt tcttctgtcg gggaaattca
    30121 agccccaaaa aacggtgcaa cgcctccagc ttttgcgcgc tggggctttt aattttggca
    30181 ttcagtaact ggcttaaata ccctttggtc ataccgctgg catcagctac ctgctgtagc
    30241 gtgcagccct gttgcttgat ggcagttttc aggtaatcaa atgacgacat atctccctcc
    30301 gtatctctca ttataagtcg tcgaacacgc taagcgcgtc ggagagtttt ttaacgccaa
    30361 aaatctgcat cccttccggc gcttttttcg gtacgttagc cgccggaaca atcgcccggc
    30421 gaaaaccgtg tttcgccgct tctgaaattc gttcctgacc gctgggcacc gggcggatct
    30481 cccctgccag cccgacttca ccaaacacca ccagatcctg tggcagcggt ctgtcacgca
    30541 ggctggaaac catcgccagc agtaacgcta aatcggcact ggtttcggtt accttcacgc
    30601 cgccgaccac gttcacaaat acatcctgat cggccatttg cagaccaccg tgacggtgca
    30661 agacagccag caggattgcc agacggtttt gttccagccc cactgccacg cggcgcggat
    30721 tcgccatcat cgagtgatcg accagcgcct gaatctccac cagcaacgga cgcgttcctt
    30781 cccacaccac catcacggag ctgccggagg tcacttcatc tccgcgactt aagaaaattg
    30841 ccgaagggtt gctgacttca cgcagcccct gttcggtcat cgcgaagacg cccagctcat
    30901 tcaccgcgcc gaagcggttt ttatggctgc gcaaggtgcg aaaacgggag tcggcatcac
    30961 catccagaag caccgaacag tcgatacagt gttccagcac tttagggcca gccagcgagc
    31021 catctttggt tacgtgccca accatgacaa tcgccacacc gcgcgttttg gcgaagcgcg
    31081 tcagataagc cgccgtttca cgcacctgcg ccacgctacc aggcgatgat tgtacatccg
    31141 ccatatgcat cacctggatc gagtcaatta ccatcagttt cggttgctct tcttcggcaa
    31201 tcaggcagat ctgttcgatg ctggtttccg acaacatatt gagattgtca gtcggcaggc
    31261 caaggcgatg agcgcgcatt gccacctgtt gcagcgactc ttcgccagtg acatacagcg
    31321 ttttcatctg ctgggccagt ttgcacagcg tttgcagtag cagcgtggat ttccccgcac
    31381 ctgggttacc gccaatcaga atggcgcttc ccggcaccac gccgccgcct agtacgcggt
    31441 cgaactcttt aaatccggtg gaaaaacgcg gcagctcttc aaggctgata tcggagagtt
    31501 tctggacttt tgccaccccg gcgctaccgg catagccgct gagacgctcg ttacgcgcca
    31561 ccgttggcga cgcagcaaga cgcacctcgg tgatggtgtt ccaggcatga caggcactgc
    31621 actgcccctg ccagcgcgga taatcggccc cgcattcatt acaaacaaag gcgcgttttg
    31681 gagcttttgc cacggtgtac ctcgttaatg ctgtgccgcc cgcaggatgg cgggcgagca
    31741 attacttctg attcaggctg ccagagagga tgcagaatac ccccatcagg tcagcgtgac
    31801 ggatggtgac ttccgccttt tcattcactt ttggcttggc atggtaggca atccccagcc
    31861 ctgctgcttt gatcatcggc aggtcattag ctccatcgcc aatcgccacg gtctgcgcca
    31921 gcgggatttc atactcctgc gcgaggcgag tcaaagtttt cgctttgtac tgcgcgtcta
    31981 cgatgtcgcc gatcacattg ccggtaaatt taccgtccat gatctccagt tcattggcta
    32041 ccacggcggt caggcgcagc ttgtcgcgca gatattcagc aaagaaagta aagccgccgg
    32101 aggcaatcgc cactttccag cccagcgttt ccagcttgag caccagttgc gttaagcctg
    32161 gcatcagcgg cagattttca cgcacctgtt gcagaatatt ggcgtcagcg cctttcagcg
    32221 tcgccacacg gctgcgcagg ctggcggtaa aatcgagttc gccgcgcatc gcccgttcgg
    32281 ttacttccgc caccatctcg cccgttccgg ccagtttggc aatttcatca atacattcaa
    32341 tctggatggc ggtggaatcc atgtccatca ccagcaaacc cggcgtgcgc aggtgcggga
    32401 ttttccccag cggggcgaca tccagttgcg cttcatgggc caggcgcgta gcccgtgcgg
    32461 tgagtgaacc tgccagacga atcacctgat aatcttccac gcaccaggcg gcaacaatca
    32521 ccatcgccgc acccagtttg ctctggtatt gggtcagacg ctgtttatcc agcccacgac
    32581 catacagcag ccagccgcta cgacctgcgt ggtaatccag tggcatcact tcatcaccac
    32641 ttaatgaaag aggcagaccc ggccataaag agacatcttc aggcaggtcg caccaggtaa
    32701 tgttaggcat taaggctcct gtaaaatcgt tcgaagcagg gaaaataacg catgaggcta
    32761 ccttgtatcc attgcttctg gcaacattaa gtctcaaatt ttcaaagggt ggaagatggc
    32821 tcgcacaaaa ctgaaattcc ggctgcatcg ggcagtgatt gtcctgttct gtcttgcctt
    32881 gttagtggca ctgatgcagg gggcgtcatg gtttagtcaa aaccaccagc gacagcgtaa
    32941 tccacagctg gaagaactgg cccgcaccct ggcgcgtcag gtgacgctga acgttgcacc
    33001 gctgatgcgt accgactcac cagatgaaaa acgcattcag gcgatcctcg atcagttaac
    33061 ggatgaaagc cgcatcctcg acgcgggtgt gtatgacgaa caaggcgatc ttatcgcccg
    33121 ttcaggcgaa agcgtcgaag tgcgcgaccg gctggcgctc gacggtaaaa aagcaggcgg
    33181 ctattttaac cagcagattg tcgagccaat tgcgggtaaa aacggaccgc tcggctatct
    33241 gcgcctgaca ctcgacaccc atacgctcgc caccgaagcc caacaggtgg ataacaccac
    33301 taacatttta cgcctgatgt tgctgctctc actggcaatc ggcgtagtgc tgacccgcac
    33361 gctgctacag ggtaaacgca cccgctggca gcaatcgccc ttcctgttaa ccgccagcaa
    33421 accggtgccg gaagaggaag aaagcgagaa aaaagagtga cccattacta caagaaagga
    33481 aatcgttatg tccacattac gcctgctcat ctctgactct tacgacccgt ggtttaacct
    33541 ggcggtggaa gagtgtattt ttcgccaaat gcccgccacg cagcgcgttc tgtttctctg
    33601 gcgcaatgcc gacacggtag tgattggtcg cgcgcagaac ccgtggaaag agtgtaatac
    33661 ccggcggatg gaagaagata acgtccgcct ggcgcggcgc agtagcggtg gcggcgcggt
    33721 gttccacgat ctcggcaata cctgctttac ctttatggct ggcaagccgg agtacgataa
    33781 aaccatctct acgtcgattg tgctcaatgc gctgaacgcg ctcggcgtca gcgccgaagc
    33841 gtccggacgt aacgatctgg tggtgaaaac agtcgaaggc gaccgcaaag tctcaggctc
    33901 ggcctatcgc gaaaccaaag atcgtggctt ccaccacggc actttgctgc tcaatgccga
    33961 ccttagccgc ctggcaaact atctcaatcc ggataaaaag aaactggcgg cgaaaggcat
    34021 tacctcagtg cgttcccgcg tgaccaacct caccgagctg ctgccgggga tcacccatga
    34081 gcaggtttgc gaggccataa ccaaggcctt tttcgcccat tatggcgagc gtgtagaagc
    34141 ggaaatcatc tccccggaca aaacgccaga cttgccaaac ttcgccgaaa cctttgcccg
    34201 tcagagtagc tgggaatgga acttcggtca ggctccggca ttctcgcatc tgctggatga
    34261 acgctttagc tggggcggcg tggaactgca tttcgacgtt gaaaaaggcc atatcacccg
    34321 cgcccaggtg tttaccgaca gcctcaaccc cgcgccgctg gaagccctcg ccgggcgact
    34381 gcaaggctgc ctgtaccgcg cggatatgct gcaacaagag tgcgaagcgc tgttggttga
    34441 cttcccggac caggaaaaag agctacggga gttgtcgacg tggatagcgg gggcggtaag
    34501 gtaactcgct gattaaaata ccaaaggaag ggaatatgat cgatctggaa aaccaggagc
    34561 gggagattat caatttaatg ttaagccagc gtatttcctg gctagccgcc gtgcgtatca
    34621 ggcataaatt atcgcttgct gaagtaagta aaatgttagg tatctcgatt aattctctaa
    34681 aatagatcga aaagacggaa cggcttagct caaacatcaa aagcaaaatg ggagaaattt
    34741 atggttgtcc accagagttg ctgatatgcc cttcctggat gacggctgaa cataaatgaa
    34801 tagattcgtg aatagatact tgaatgtatt ttatttttta tccatatgaa aaataatagt
    34861 aaatctataa atcagttgct attgatgaat agatactaaa aagccggagc agtcccccac
    34921 tccggcttaa cacgacaatc gcctttcagc gaaacacaat tactctttat cgcccagcag
    34981 aacggattcc agtgcgattt tgatcatgtc gttgaaggtg gtctgacgct cagcggcagt
    35041 ggtctgctcg tgagtgcgga tgtggtcaga cacggtgcag atggtcaggg ctttcgcgcc
    35101 aaattctgca gcgacgccgt agataccagc cgcttccatt tccacgccga ggatgccgta
    35161 tttttccatc acgtcgaaca tttcgccgtc cggagagtag aacaggtcag cggagaacag
    35221 gttaccaacg cgagcatcaa tgcccagtgc tttagccgcg tctaccgcgt tacgcaccat
    35281 gtcaaagtca gcgatagcgg caaagtcatg atctttaaaa cggatgcggt taactttgga
    35341 atcggtgcag gcacccatac cgataacgac gtcgcgcagt tttacgtgcg gcagaactgc
    35401 gccacaggaa cccacgcgga taattttctt cacgccgaaa tcggtgatca gttctttggt
    35461 gtagatggag caggacggga tacccatacc gtgacccatt acggaaattt tgcggccttt
    35521 gtaagtaccg gtgaagccca gcataccgcg aacgttgttc acttcacggg catcttcaag
    35581 gaaagtttca gcaatatact tcgcacgcag cgggtcgcct ggcatcaaaa ctacgtcagc
    35641 gaaatcgccc atttctgcat taatgtgtgg ggtagccatt gttttatcct tatcaacatg
    35701 ttttcaccct ctccctgagg gagagggccg gggtgagggc atcaagccgc acccattatc
    35761 agaacatggc tttgccatat tccatatcag aagtaccaaa atattttgcc agagtctggc
    35821 cgatatccgc gaaggtttca cggtgaccca gtgagcccgg ttttactttc gggccgtaaa
    35881 ccagtaccgg aatgtgttca cgcgtgtggt cagtaccggt ccaggtcgga tcacaaccgt
    35941 ggtcagcagt gaggatcagg atgtcgtcat cgcgcagcag agacatcagc tccggcagac
    36001 ggcggtcgaa cagctccaga cccacagcat aaccggcgac gtcgcgacgg tggccccagg
    36061 aagagtcgaa gtcaacgaag ttggtgaaga cgatggtgtt atcacccgct tctttcatct
    36121 ctttgatggt ggcgtcaaac agcgcgtcca ggccagttgc tttcactttc ttggtgatac
    36181 cgcagttggc gtagatgtct gcaattttac cgacagaaac cacctgaccg tgtttttcat
    36241 caaccagttt ctgcagcacg gtcggtgctg gcggctcaac agccaggtcg tgacggttac
    36301 cggtacgctg gaagttaccg gctttgtcac cgataaacgg acgagcgata acacgaccga
    36361 tgttgtagcc gccgttggtc agctcttcac gggcgatttc gcacagttcg tagagtttat
    36421 ccaggccaaa ggtttcttcg tggcaggcaa tctggaacac agagtcagcg gaggtataga
    36481 aaatcggctt gccggttttc atgtgctctt cgcccagctg atccagaatg accgtaccgg
    36541 aagagtggca gttaccgagg taacccggca gattagcgcg ttcaaccagt ttatccagca
    36601 gctcttgcgg gaagctgttt tcgtgatcgg agaaatatcc ccactcaaat agaaccggga
    36661 caccggcgat ttcccagtga ccagacgggg tatctttacc ggatgacatt tcgtgcgccc
    36721 atgcgtacgc gccgataact tcagcgttgc cgtccattcc cgccggaatg aaaccggtag
    36781 aaccttcgtg tgctttcgcc agccccagac gggtcagatt tggcagattg agcggacctt
    36841 tacgaccgtt atcagcttcg cctttggcac aagcttctgc gatatgaccc agggtgtcag
    36901 ccccgacgtc accaaagcgt tctgcatctt ctgtagcgcc gatgccgaat gagtccagca
    36961 ccataataaa tgcacgtttc atatgttctc cgtactcagt gcttcaaatt taaatgatca
    37021 gatcagtata ccgttattcg ctgatacggc gatagacagt tggtgtgctt tccggtgctt
    37081 tatcggcaag tttaattgcc gctttcaccg ctttcgccgc ttcctgccag ctgttttcgt
    37141 ctttcgcgtg gataaccgcc agcggacgct gaccgtctac ctggtcgccc aaacgcgcca
    37201 tatcagtaaa gccgacgctg taatcaatgg tgtcagatgc ctgacgacga ccgccgccca
    37261 ttgcaaccac tgccatcccc agcgcgcggg tatccatttc actgacaaaa ccttcggtat
    37321 cagcatagac tgctttcgtc agcatcgctg tcggcagata cttcgcgtag ttctcaacga
    37381 agtcggttgg gcctttttgt gccgctacca tacgaccaaa gacttctgcc gctttaccgt
    37441 tgtccagcac tgcttgcaat ttcgcgcgcg cttcggcgtc atctttcgcc agtttgccgg
    37501 agataagcat ctccacgcac agcgccatcg tgacatcaaa cagacgcggg ttacgatact
    37561 cacccgtcag gaactgcacc gcttcacgaa cttcaaccgc gttacctgca ctggaggcca
    37621 gtacctgatt catgtcggtg agcagcgcgg tggtgcgcac gccagcgccg ttagccacgc
    37681 caacaatcgc ttcggcaagg gcttcagaga gttcgtaggt cggcataaac gcgccgctac
    37741 ccactttcac gtccatcacc agcgcgtcca gaccttccgc aagtttcttc gccagaatag
    37801 aggcggtgat cagcgggatg gagtccacgg ttgcggtaat atcacgggtc gcgtagaaac
    37861 gtttatccgc cggagccagt gagctggtct gaccgataat cgccacgccg acgtctttaa
    37921 taatttcgcg gaaacggttg tcatccggga aaatgtcgaa gccagggatg gattccagtt
    37981 tgtcgagcgt accgccagta tgaccgaggc cgcgaccaga gatcatcgga atatagccgc
    38041 cgcaggctgc gaccatcggc cccaacatca gcgaagtcac atcgccgacg ccaccagtgg
    38101 aatgtttatc aacaatcggg ccattcagat gcaggctttt ccagtcgaga acggttcctg
    38161 aatctcgcat cgccatggtc agcgagacac gctcaggcat tgtcatatcg tggaagaaaa
    38221 tggtcatcgc gagggcggca atctgccctt cggagatagt gttgtcgcga atgccgttga
    38281 taaagaaacg aatttcttca tcgctcagtg catgaccatc acgtttttta cgaataattt
    38341 cttgtgcgag aaacaaggta acctcccgag gaaaagagta tgaggcggct tttgccggat
    38401 gcgtctacgc cttatccggc ctacgggtag atacgtctcg taggcctgat aagcgcagcg
    38461 catcaggcgt aaagcatctt acttagtagc tgctggcgct cttaccgtca ccgtggccca
    38521 gcgctttcaa caggcttgcc agcaggctgg aagcaccaaa gcggtagtga cgcgcatctg
    38581 cccagtcagc accgaacagt tcatctgcga tggcgagata tttctgcgca tcttccgcag
    38641 tacgcacgcc gcccgctggt ttgaaaccaa cggttttttc tacgcccata tcacggatca
    38701 cttccatcat gatgcgcgcg ctttccggcg tcgcgttcac agccacttta ccggtagagg
    38761 ttttgatgaa gtccgcaccc gctttgatgg agatttcaga cgctttacgg atcagcgctt
    38821 cgtctttcag ttcgccggtt tcgatgatca ctttcagcag tacattcgct gccgcgcaag
    38881 cctctttaca ggctttcacc aggtcaaaac caacctgctc gttacccgcc atcagcgcgc
    38941 ggtacgggaa cacaacgtca acttcatcag caccgtaggc gattgccgca cgggtttctg
    39001 ccagcgcgat ttcgatgtca tcgttaccgt gtgggaagtt ggttaccgta gcgattcgga
    39061 tttccggggt gccctgctct ttcagcgttt tgcgagcaat cgggataaag cgaggataga
    39121 tacagatagc ggcggtattg ccgaccgggg ttttggcctg atgacacaga gcaattactt
    39181 tctcgtcggt gtcgtcgtca ttcagggtgg tcaggtccat caatttcagt gcacgcaggc
    39241 tgcttgcttt caaatcagtc atttcattct cctggcttgt cgccaataaa attcaccttg
    39301 cgagtttgtt agtattctaa catctactcc gcaacacact tcgatataca tcacaattaa
    39361 ggaaatctac ttacaagttt gcatcactgt aatgcgatct ggttcaaata attcactttc
    39421 aaatgaatgc gtcagtggtg gcaaacgcat caggatcaaa gtgaacatca cgaaacttct
    39481 tacaatggcg catcaccttc ggcataaaag gaaaagaaga tgcccacttc tcatgaaaat
    39541 gcactgcaac aacgttgcca gcaaattgtc accagcccag tgcttagccc ggagcagaag
    39601 cgccattttc tggcactgga agcagaaaac aatctgcctt atccgcagct tcctgccgaa
    39661 gcccgccgcg cgctggatga gggtgtaatc tgcgatatgt ttgaaggtca tgcgccgtac
    39721 aaaccgcgct atgtcttacc cgattacgcc cgttttctgg cgaacggttc cgaatggctg
    39781 gagctggaag gcgcgaaaga tcttgatgac gcactctctc tgctgaccat tctttaccac
    39841 cacgtaccgt cggtcacatc gatgccggtc tacctggggc aactggatgc gttgttgcaa
    39901 ccatatgtta gaattctaac acaagacgag atcgatgttc gaataaaacg tttctggcgt
    39961 tacctcgaca gaaccctgcc agacgccttt atgcacgcca atatcggccc gtctgattcg
    40021 cccattaccc gcgcgatctt acgtgcagac gcagagttga agcaggtttc accgaacctg
    40081 acctttatct acgatcctga aatcacccct gatgacctgc tgctggaagt ggcgaagaac
    40141 atctgtgaat gtagcaaacc gcacatcgcc aacggtccgg tgcatgataa aattttcaca
    40201 aaagggggct acgggattgt gagctgttac aactcactgc cgctggcggg tggtggcagc
    40261 acgctggtac gccttaacct gaaagccatt gccgagcgca gcgaatcgct ggatgacttc
    40321 tttacgcgca ctctaccgca ctactgccag cagcagatcg ccatcatcga tgcgcggtgt
    40381 gaattcctct atcaacaatc acacttcttt gagaatagct tcctggtgaa agaagggctg
    40441 attaaccctg aacgttttgt gccaatgttt ggcatgtatg ggctggcgga agcggttaac
    40501 ttgctgtgta agaaagaagg gattgccgcg cgctacggta aagaagccgc cgcaaatgaa
    40561 gtgggttatc gcatcagcgc gcaactggcg gagtttgtcg ccaatacccc cgtgaaatat
    40621 ggctggcaaa aacgcgccat gttacacgca cagtcgggga tcagttccga tatcggcacc
    40681 acgccgggcg cgcgtttgcc gtatggtgat gagccagatc cgatcaccca tctgcaaact
    40741 gtcgccccgc atcatgctta ttattattcc ggcatcagcg acattctgac gctcgacgaa
    40801 accatcaaac gtaatccgca ggcgctggta cagctttgcc tcggtgcctt taaagccgga
    40861 atgcgtgaat ttactgccaa tgtcagcggt aacgatctgg ttcgcgttac cggttatatg
    40921 gtgcgtttgt cggatttaga aaaatatcgc gccgaaggtt cacgcaccaa caccacctgg
    40981 ctgggcgaag aagccgcacg caacactcgt attctggaac gccagccgcg cgtgataagc
    41041 catgaacagc agatgcgctt tagtcagtaa gattatcccc ttctcctgcg ttgacgggcc
    41101 aggcagtcgt ctggctctgt ttttgcaggg ctgcaatctg cgctgcaaaa actgtcacaa
    41161 tccgtggacg atgggacgtt gcaatgactg tggggagtgc gtgccacagt gtccgcatca
    41221 ggcgttgcag attgttgacg gcaaagtggt gtggaacgct gtggtttgcg agcagtgtga
    41281 tacctgcctg aagaggtgtc cgcaacatgc cacgccaatg gcgcaatcca tgagcgtgga
    41341 cgaagtgctt agccatgtcc gcaaagcggt gctgtttatt gaagggataa cggtaagcgg
    41401 cggcgaagcc acgacccaac tgccgtttgt agtggcgctg tttactgcta tcaaaaacga
    41461 tccgcaactg cgccacctca cctgcctggt ggacagtaac ggcatgttga gcgaaaccgg
    41521 ctgggaaaaa ttactcccgg tgtgtgacgg cgcaatgctc gatctcaaag cgtgggggag
    41581 cgaatgtcat caacacttaa ccggacgcga taatcagcag attaagcgca gcatctgttt
    41641 gcttgcagag cgcggcaagc tggcggaact gcgtttgctg gtaattccag accaggtgga
    41701 ttatttgcat cacatcgatg agctggcgac gtttatcaag agacttggcg atgttccggt
    41761 tcgcctgaat gcgtttcatg cccacggcgt gtatggcgag gcgcaaagct gggcgagcgc
    41821 cacgccggaa gacgttgagc cgttggctga tgcgttaaag gtgcgcgggg tgagccggtt
    41881 gatatttccg gcgctctatt tgtgacatgc cggatacggc ctacggttcg gcacagactt
    41941 gtaggcatga taagacgcga caagcgtcgc atcaggcatt atgcgccgac tgccggatgc
    42001 ggcgtgaacg
//
LOCUS       DAKISA010000028        41943 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.028, whole genome shotgun sequence.
ACCESSION   DAKISA010000028 DAKISA010000000
VERSION     DAKISA010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41943
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.028"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(234..1574)
                     /gene="fadL"
                     /locus_tag="OVW45_001539"
     CDS             complement(234..1574)
                     /gene="fadL"
                     /locus_tag="OVW45_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCV5526023.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGTSITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            1946..2230
                     /locus_tag="OVW45_001540"
     CDS             1946..2230
                     /locus_tag="OVW45_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCV5526024.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            2410..3720
                     /gene="fadI"
                     /locus_tag="OVW45_001541"
     CDS             2410..3720
                     /gene="fadI"
                     /locus_tag="OVW45_001541"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCV5526025.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            3720..5864
                     /gene="fadJ"
                     /locus_tag="OVW45_001542"
     CDS             3720..5864
                     /gene="fadJ"
                     /locus_tag="OVW45_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCV5526026.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            6067..6552
                     /gene="sixA"
                     /locus_tag="OVW45_001543"
     CDS             6067..6552
                     /gene="sixA"
                     /locus_tag="OVW45_001543"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCV5526027.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            7236..7799
                     /locus_tag="OVW45_001544"
     CDS             7236..7799
                     /locus_tag="OVW45_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCV5526028.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTCAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            7881..10523
                     /gene="yfcU"
                     /locus_tag="OVW45_001545"
     CDS             7881..10523
                     /gene="yfcU"
                     /locus_tag="OVW45_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HCV5526029.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDI
                     KPVIQEQTQQVTPTNPPVSVSANQ"
     gene            10543..11294
                     /locus_tag="OVW45_001546"
                     /pseudo
     CDS             10543..11294
                     /locus_tag="OVW45_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            11269..11817
                     /locus_tag="OVW45_001547"
     CDS             11269..11817
                     /locus_tag="OVW45_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCV5526030.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            11814..12284
                     /locus_tag="OVW45_001548"
     CDS             11814..12284
                     /locus_tag="OVW45_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCV5526031.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTLSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLMVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            12281..12805
                     /locus_tag="OVW45_001549"
     CDS             12281..12805
                     /locus_tag="OVW45_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCV5526032.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            12792..13664
                     /locus_tag="OVW45_001550"
     CDS             12792..13664
                     /locus_tag="OVW45_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCV5526033.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVTSYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(13786..14337)
                     /gene="smrB"
                     /locus_tag="OVW45_001551"
     CDS             complement(13786..14337)
                     /gene="smrB"
                     /locus_tag="OVW45_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCV5526034.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            14503..15435
                     /gene="prmB"
                     /locus_tag="OVW45_001552"
     CDS             14503..15435
                     /gene="prmB"
                     /locus_tag="OVW45_001552"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCV5526035.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            15470..16555
                     /gene="aroC"
                     /locus_tag="OVW45_001553"
     CDS             15470..16555
                     /gene="aroC"
                     /locus_tag="OVW45_001553"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCV5526036.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            16559..17383
                     /gene="mepA"
                     /locus_tag="OVW45_001554"
     CDS             16559..17383
                     /gene="mepA"
                     /locus_tag="OVW45_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCV5526037.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFAPLKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            17383..18192
                     /locus_tag="OVW45_001555"
     CDS             17383..18192
                     /locus_tag="OVW45_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCV5526038.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            18192..18740
                     /gene="epmC"
                     /locus_tag="OVW45_001556"
     CDS             18192..18740
                     /gene="epmC"
                     /locus_tag="OVW45_001556"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCV5526039.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            18774..19052
                     /locus_tag="OVW45_001557"
     CDS             18774..19052
                     /locus_tag="OVW45_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCV5526040.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(19173..21179)
                     /gene="mnmC"
                     /locus_tag="OVW45_001558"
     CDS             complement(19173..21179)
                     /gene="mnmC"
                     /locus_tag="OVW45_001558"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCV5526041.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLTALNPNRLWVRKLLKGKAVKAG"
     gene            21338..22558
                     /gene="fabB"
                     /locus_tag="OVW45_001559"
     CDS             21338..22558
                     /gene="fabB"
                     /locus_tag="OVW45_001559"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCV5526042.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            22833..24011
                     /locus_tag="OVW45_001560"
     CDS             22833..24011
                     /locus_tag="OVW45_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCV5526043.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTEFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(24008..25003)
                     /gene="flk"
                     /locus_tag="OVW45_001561"
     CDS             complement(24008..25003)
                     /gene="flk"
                     /locus_tag="OVW45_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCV5526044.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVNDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            25102..26238
                     /gene="pdxB"
                     /locus_tag="OVW45_001562"
     CDS             25102..26238
                     /gene="pdxB"
                     /locus_tag="OVW45_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCV5526045.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            26304..27317
                     /locus_tag="OVW45_001563"
     CDS             26304..27317
                     /locus_tag="OVW45_001563"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCV5526046.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            27317..28129
                     /gene="truA"
                     /locus_tag="OVW45_001564"
     CDS             27317..28129
                     /gene="truA"
                     /locus_tag="OVW45_001564"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCV5526047.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            28212..28871
                     /locus_tag="OVW45_001565"
     CDS             28212..28871
                     /locus_tag="OVW45_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCV5526048.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            29027..29941
                     /gene="accD"
                     /locus_tag="OVW45_001566"
     CDS             29027..29941
                     /gene="accD"
                     /locus_tag="OVW45_001566"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCV5526049.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVHRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            30011..31279
                     /gene="folC"
                     /locus_tag="OVW45_001567"
     CDS             30011..31279
                     /gene="folC"
                     /locus_tag="OVW45_001567"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCV5526050.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            31269..31931
                     /gene="dedD"
                     /locus_tag="OVW45_001568"
     CDS             31269..31931
                     /gene="dedD"
                     /locus_tag="OVW45_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCV5526051.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            32190..32678
                     /gene="cvpA"
                     /locus_tag="OVW45_001569"
     CDS             32190..32678
                     /gene="cvpA"
                     /locus_tag="OVW45_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCV5526052.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            32715..34232
                     /gene="purF"
                     /locus_tag="OVW45_001570"
     CDS             32715..34232
                     /gene="purF"
                     /locus_tag="OVW45_001570"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCV5526053.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            34327..34896
                     /gene="ubiX"
                     /locus_tag="OVW45_001571"
     CDS             34327..34896
                     /gene="ubiX"
                     /locus_tag="OVW45_001571"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCV5526054.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            35162..35944
                     /gene="argT"
                     /locus_tag="OVW45_001572"
     CDS             35162..35944
                     /gene="argT"
                     /locus_tag="OVW45_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCV5526055.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKAFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            36165..36947
                     /gene="hisJ"
                     /locus_tag="OVW45_001573"
     CDS             36165..36947
                     /gene="hisJ"
                     /locus_tag="OVW45_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCV5526056.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            37037..37723
                     /gene="hisQ"
                     /locus_tag="OVW45_001574"
     CDS             37037..37723
                     /gene="hisQ"
                     /locus_tag="OVW45_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCV5526057.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            37720..38436
                     /locus_tag="OVW45_001575"
     CDS             37720..38436
                     /locus_tag="OVW45_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCV5526058.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            38444..39217
                     /gene="hisP"
                     /locus_tag="OVW45_001576"
     CDS             38444..39217
                     /gene="hisP"
                     /locus_tag="OVW45_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCV5526059.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            39414..40304
                     /gene="rpnB"
                     /locus_tag="OVW45_001577"
     CDS             39414..40304
                     /gene="rpnB"
                     /locus_tag="OVW45_001577"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCV5526060.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLESNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKLEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(40352..41245)
                     /locus_tag="OVW45_001578"
     CDS             complement(40352..41245)
                     /locus_tag="OVW45_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCV5526061.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(41266..41628)
                     /gene="folX"
                     /locus_tag="OVW45_001579"
     CDS             complement(41266..41628)
                     /gene="folX"
                     /locus_tag="OVW45_001579"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCV5526062.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            complement(41685..>41943)
                     /locus_tag="OVW45_001580"
     CDS             complement(41685..>41943)
                     /locus_tag="OVW45_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HCV5526063.1"
                     /translation="ERYQVETQRLYHVLNKRLENSPWLGGENYSIADIACWPWVNAWT
                     RQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
BASE COUNT         9950 a        10291 c        11232 g        10470 t
ORIGIN      
        1 gtatcctgga aatgcagaaa agtaaactct ttgagaagga cgagcagaac gcctgaaaat
       61 atgcctttcc cttcaggttt gtctgtggat accgcttatt gatttgaaag ggcaagctgc
      121 caacaggctg tttgcaaaac tcaggaggta agcaatgagg acactacttt cggtgaagtg
      181 ggggagttaa agtcacctgc tatgcaggtg actttatcca ggcgaacgcg ttatcagaac
      241 gcgtagttaa agttagtacc gaacagccag gctttacctt cagactcgaa ctggtatggg
      301 ccttcgttaa ttttcacgct ctgaccgtgc atataagaaa caccaacgtc gactgaagca
      361 tctttattaa atgcgtaagt cgtacctgca ctcagccaga aacggtcctg gtccggaatg
      421 gagatagaac gattctgagc cggaactggg ctgtcatcaa aggcgatacc ggtacggaag
      481 gtccagttat catcgtagta ataagtggta cccaacgcga tgcggtaagc atctttaaag
      541 ccttcatgtt tctggaacag cgtgtcgcca ctggttgagg tcgctttcag ctgctggaac
      601 tgactccagc tggtgtaagc caggctatag tgaatcgccc actgtggatc aacacggtta
      661 taacctgaca cttcccacat ttcaggcagg ttcagcgtca gataacccga ttgcgttgcg
      721 ccacctgtcg cggtaggaat tggcaaaccg tagttattaa acgcacgatt aagatcgctg
      781 ctgtagttac ctttgaagtc aattttcact tcagaacggt aggtcagtgc atagcggtta
      841 tttttatcca gttcatacag gattccggcg ttccagccaa agccccactg gttaccgttc
      901 agatgagcga ttttggtatt actgtcgata ccgttggcgg tagctgccaa tgcttgccct
      961 tgaggagttt gcccggcagg agattgcata atctgaccag caaccaactg ccccagatcg
     1021 cctgcgaaac gttcaatttt cgcgcgagcg tagacggcgt tgaaaccaag accaaagctc
     1081 catgcattat ttaagcgata cgcaccgctt aagttcaggt tcatggtttc aaggtcagtt
     1141 gtacccccga cagagccgcc tgcataagta tcattaaact ctgttgccag accatagtta
     1201 gaggtaatag aagtgcccca accaaattgg tcgttaatcg gtgcaacaaa gtgcatgttc
     1261 ggaacccatg ccgtaggcgc gatgttatcg gctttcaggc tacgaccaga cggagacgtt
     1321 ccgctgatat tcacatccgg gtcaatataa accgcacccg cagaaaatgt cgggcggtca
     1381 aacatggtaa tcaatgcggg gttacggcta acgttacctg catcatcggc aattgcgcct
     1441 tcccctgaat aagcccggcc caggccagag gaagaaaatt cgtttaactg aaagcctgcc
     1501 gaccaggcct gggtggagat aagtgccact gcgactgcga gagcagactt tgtaaacagg
     1561 gttttctggc tcatgaccat aacctcaatg atttattttt atacaaacta tgttacgtgc
     1621 tgtaacagga gcgcgaagtg tagggtctga ggtacatctt agaaatcaga ccagtggcga
     1681 gagtataggt cggaccagct ggaatgctgc aagtatgttt ccatcttttt tcaaatatga
     1741 tctaagaaac gcgatccagc tggcaaaatt tggaggttgc gcactcgcgg attcgggtga
     1801 attttgtttt agatcatttt taagtgtgat ttcggtcact tatccgattt tgcagaatta
     1861 acaactggag cagcactttc cggaagcgta aaatatccac aacgtttatc tggcaccttg
     1921 ggtgcacata cagaggaaat caacgatgag taaatgtagt gctgatgaaa ccccggtttg
     1981 ctgctgtatg gatgttggca ccattatgga caactccgac tgcaccgcgt cttacagccg
     2041 cgtgtttgca aaccgcgcag aagcagaaca aacgctggcg gcgctgactg aaaaagcccg
     2101 tagcgtggaa tccgaaccgt gcaaaatctc cccgactttc actgaagagt ctgacggtgt
     2161 gcgcctggat atcgatttca cttttgcctg cgaagctgaa atgctgatct tccagctggg
     2221 tctgcgttaa ttcctctcgg gtacgttcgc ccctgtcagt tgctggcagg ggcgttttct
     2281 tttctttctc ctgtcttcac tgtgtcatag ctcccacttt cctttttctc cgcttggcta
     2341 aatgtaaaaa aatagttaag actatgatca ggtcagacca ctttatttat ttttttacag
     2401 gggagtgtta tgggtcaggt tttaccgctg gttacccgcc agggcgatcg tatcgccatt
     2461 gttagcggtt tacgtacgcc ttttgcccgc caggcgacgg cttttcatgg cattcccgcg
     2521 gttgatttag ggaagatggt ggtaggcgaa ctgctggcac gcagcgagat ccccgctgaa
     2581 gtgattgaac agctggtctt tggtcaggtc gtacaaatgc ctgaagcccc caacattgcg
     2641 cgtgaaattg ttctcggtac cggaatgaat gtgcataccg atgcttacag cgtcagccgc
     2701 gcttgcgcta ccagtttcca ggcggttgca aacgtcgcag aaagcctgat ggcgggaact
     2761 atccgagcgg ggattgccgg tggggcagat tcctcttccg tattgccaat tggcgtcagt
     2821 aaaaaactgg cgcgcgtgct ggttgatgtc aacaaagctc gaaccatgag ccagcgcctg
     2881 aaactcttct ctcgcctgcg tttgcgcgac ttaatgcccg taccgcctgc ggtagcagaa
     2941 tattctactg gcttgcggat gggcgacacc gccgaacaaa tggcgaaaac ctacggcatc
     3001 acccgagaac agcaagatgc attagcgcac cgttcgcatc agcgtgccgc tcaggcatgg
     3061 tcagacggaa aactcaaaga agaggtgatg actgccttta tccctcctta taaacaaccg
     3121 cttgtcgaag acaacaatat tcgcggtaat tcctcgcttg ccgattacgc aaagctgcgc
     3181 ccggcgtttg atcgcaaaca cggaacggta acggcggcaa acagtacgcc gctgaccgat
     3241 ggcgcggcag cggtgatcct gatgactgaa tcccgggcga aagaattagg gctggtgccg
     3301 ctggggtatc tgcgcagcta cgcatttact gcgattgatg tctggcagga catgttgctc
     3361 ggtccagcct ggtcaacacc gctggcgctg gaacgtgccg gtttgacgat gagcgaactg
     3421 acattgatcg atatgcacga agcctttgca gctcagacac tggcgaatat tcagttgctg
     3481 ggtagtgaac gttttgctcg tgaagtactg gggcgtgcac atgccactgg cgaagtggac
     3541 gatagcaaat ttaacgtgct tggcggttcg attgcttacg ggcatccctt cgcggcgacc
     3601 ggcgcgcgga tgattaccca gacattgcat gaacttcgcc gtcgcggcgg tggatttggt
     3661 ttagttaccg cctgtgctgc cggtgggctt ggcgcggcaa tggttctgga ggcggaataa
     3721 tggaaatggc atcagcgttt actcttaatg ttcgtctgga caacattgct atcatcacca
     3781 tcgatgtacc ggatgagaaa atgaataccc tgaaggcgga atttgcctcg caggtgcgcg
     3841 ccattattaa gcaactccgt gaaaacaaag agttgcgcgg cgtggtgttt atctccgcga
     3901 aaccggacaa ttttatcgcc ggagcagaca tcaacatgat tggcaactgc aaaacggcgc
     3961 aagaagcgga agcactggcg cggcagggcc aacagttgat ggcggagatt catgctttgc
     4021 ccattccggt tatcgcggct attcatggcg cttgcctggg cggtgggctg gagctggcgc
     4081 tggcgtgcca cggtcgcgtt tgtactgacg atcctaaaac ggtgctcggt ttgcctgaag
     4141 tacaacttgg attgttaccc ggttcaggcg gcacccagcg tttaccgcgt ctgataggcg
     4201 tcagtacagc attagagatg attctcaccg gaaaacaact tcgggcgaaa caggcattaa
     4261 agctggggct ggtggatgac gttgttccgc actccatttt gctggaagcc gctgttgagc
     4321 tggcgaagaa ggatcgccca tcttcccgcc ctctacctgt acgcgagcgt attctggcgg
     4381 ggccgttagg tcgtgcgctg ctgttcaaaa tggtcggcaa gaaaacagaa cacaaaactc
     4441 aaggcaatta tccggcgaca gaacgcatcc tggaggttgt tgaaacggga ttagcgcagg
     4501 gcaccagcag cggctatgac gctgaagctc gcgcctttgg cgaactggcg atgacgccgc
     4561 aatcgcaagc tttgcgtagt atcttttttg ccagtacgga cgtgaagaaa gatcccggta
     4621 gtgatgcacc gcctgcgcca ttaaacagcg tgggaatttt aggtggtggc ttgatgggcg
     4681 gcggtattgc ttatgtcact gcttgtaaag cggggcttcc ggtcagaatt aaagatatca
     4741 acccgcaggg cataaatcat gcgctgaagt acagttggga tcagctggag ggcaaagttc
     4801 gccgtcgtca tctcaaagcc agcgaacgtg acaaacagct ggcattaatc tccggaacga
     4861 cggactatcg cggctttgcc catcgcgatc tgattattga agcggtgttt gaaaatctcg
     4921 aattaaaaca acagatggtg gcggaagtgg agcaaaattg cgccgctcat accatttttg
     4981 cttcgaacac gtcatcttta ccgattggtg atatcgccgc tcacgccgcg cgacctgagc
     5041 aagttattgg cctgcatttc ttcagtcctg tggaaaaaat gccgctggta gagattattc
     5101 ctcatgcggg tacatcggcg caaaccatcg ccactacggt aaaactggcg aaaaaacagg
     5161 gcaaaacgcc cattgtcgtg cgtgacaaag ccgggtttta cgtcaatcgc atcttagcgc
     5221 cttacattaa tgaagctatc cgcatgctga ccgaaggtga acgggtagag cacattgatg
     5281 ccgcgctagt gaaatttggt tttccggtag gcccaatcca acttttggat gaggtaggaa
     5341 tcgacaccgg gactaaaatt attcctgtac tggaagccgc ttatggagaa cgttttagcg
     5401 cgcctgcaaa tgttgtttct tcaattttga acgacgatcg caaaggcaga aaaaatggtc
     5461 ggggtttcta tctttatggt cagaaagggc gtaaaagcaa aaaacaggtc gatcccgcca
     5521 tttacccgct cattggcgca caagggcagg ggcgactctc cgcaccgcag gttgctgaac
     5581 ggtgtgtgat gttgatgctg aatgaagctg tacgttgtgt tgatgagcag gttatccgta
     5641 gcgtgcgtga cggggatatt ggcgcggtat ttggcattgg ttttccgcca tttctcggtg
     5701 gaccgttccg ctatatcgat tctctcggcg cgggcgaagt ggttgcaata atgcaacgac
     5761 tggccacgca gtatggttcc cgttttaccc cttgcgagcg tttggtcgag atgggcgcgc
     5821 gtggggaaag tttttggaaa acaactgcaa ctgaccttca ataagaaggt caaagctata
     5881 tgaatccgcg ctgaatggcg gagtgttggt caaaatgtaa acgcatattg actatactta
     5941 cgccattgag gtaaaaaaca gcgtttcatt cggtgaatgg ataaggcaca atgccggcca
     6001 ccgttttctt tctctggttt cagatgaaag aaaacggacg aatctggtta acaaaagcgg
     6061 tgcaatatgc aagtttttat catgcgtcac ggcgacgcag ccctcgatgc cgccagtgat
     6121 tcggttcgtc ctctgaccac taatggttgt gacgaatctc gcctgatggc gaactggctg
     6181 aaaggtcaaa aagtggaaat cgaacgcgtt ctggtgagcc cgttcctgcg agccgagcaa
     6241 acactggaag aagtagggga ttgtctgaac ctgccttcca gtgcagaagt tctgccggag
     6301 ttaacgccct gcggcgatgt tggtctggtc agtgcctatt tgcaggcgtt gaccaatgaa
     6361 ggtgttgctt cggtactggt gatctctcac ttaccgttag tcgggtatct ggttgccgag
     6421 ttatgtccgg gcgaaacgcc gcctatgttt accacctcgg ccattgccag cgtaacgctt
     6481 gatgaaagcg gaaacggcac attcaactgg cagatgagcc cgtgtaatct gaagatggca
     6541 aaagctatct gatgagttcc aaattatgcc caatgaggta gattcattgg gcataattgc
     6601 tcatcaggca gcggatatct gtcaatctca ctactatcga agactctccc catctataaa
     6661 taaaacactt tctactttca ttcggaaatt attcttctgc gcgtatcttg attttgccca
     6721 aagttacatg ttgaaatgaa taatgtctgt ttgacactca ttgtcattta ttaatacaga
     6781 taatagagaa aatagagttg aataatcagg caggatgtaa ttgaaacttg attaaattct
     6841 ggctatagtg tttatatatt atattgtcaa aagtggataa atgccaatcg cattatatgt
     6901 tcattgtttg ttttttaagc tgttgatatt cattattgat atactttcta ttttttctaa
     6961 atacttattc cgtatttttt aaggatgtcc ttaagataac ctttcttctg tcaatatatt
     7021 aattaaatct acatttactc tttagtaaag ttaaattgca tcaggggtgt ggaaacatca
     7081 ctatgcggcc ccacaccaac gaatacaatt ttctacgacc tgttcttttg ggatcgcatg
     7141 ccgtattcaa tggctgggta taaacattgg ttattgactg aatcaatgta ttgcaatgtt
     7201 tatattttta attatttata gtttaaggac aaataatgag taagtttgtt aaaacagcta
     7261 ttgctgcggc aatggtgatg ggcgtgttca cctctacggc aacaatcgct gcgggtaaca
     7321 acggtactgc acgtttttac ggcaccattg aagactcagt gtgttctatc gttccggacg
     7381 atcacaaact ggaagttgat atgggtgata tcggtgccga aaaactgaaa aataacggca
     7441 ccaccacgcc gaaaagtttc cagattcgtc tgcaagattg cgtatttgat actcaggaaa
     7501 caatgaccac tacctttact ggtaccgttt cttctgcaaa tagcggcaat tattacacca
     7561 ttttcaatac cgatacctgt gcggcattta acaatgttag cctggcgatc ggtgactctc
     7621 tgggtacctc ttacaaaagc ggcatgggta ttgaccagaa aatagtgaaa gacacttcta
     7681 ccaataaagg caaagcgaag cagacactga actttaaagc ctggctggtc ggcgcagctg
     7741 atgcgccaga tctgggtaat ttcgaagcaa ataccacctt ccagattact tacctgtaag
     7801 gcataccccc atagcgacac tgctatgggg gatttaaatg gattttgacc gaacaagaat
     7861 aaaaacggat agaaacgtgt atgcctgacc attctctttt tcgattacgg attcttccgt
     7921 ggtgcattgc gctggcaatg tcagggagtt atagcagtgt ctgggctgaa gacgacattc
     7981 agtttgattc ccgttttctg gaattaaaag gcgacacaaa aattgatctg aagcgttttt
     8041 ccagccaggg atatgttgag cccggaaaat acaatttaca ggttcaacta aataaacagc
     8101 cattggcgga agagtacgat atttactggt atgctggtga agatgacgcg agcaaaagct
     8161 atgcttgtct gacaccggaa ctggtagcgc agtttggttt aaaagaagac gtggcgaaaa
     8221 atctgcaatg gagccacgat gctaaatgcc tgaaatccgg tcaactggaa ggcatggaaa
     8281 ttaaggctga tttaagccag tccgcattag tcatttcact gccacaggct tacctcgaat
     8341 atacttatcc cgactgggat ccgccttcac gttgggatga cggcatctcc gggatcgtcg
     8401 cggactacag catcaacgca caaacccggc acgaagaaaa tggcggtgat gatagtaacg
     8461 agatcagcgg caacgggacg gtcggggtta acctggggcc gtggcgtatg cgtgctgact
     8521 ggcagactaa ctatcaacat actcgcagta atgatgacga tgaattcagc ggcgatgaaa
     8581 ctcaaaaaaa atgggagtgg agtcgctact atgcctggcg ggcgttacca tcattaaaag
     8641 ccaaactggc gctgggcgag gattacctca gatccgatat ttttgatggt tttaactatg
     8701 ttggtggcag tgtcagtact gacgatcaaa tgttgcctcc caatctgcgc ggctacgcgc
     8761 cagacatttc cggcgtggca cacaccacag caaaagtgac cgtcagccag atggggcgtg
     8821 tgatttacga aacgcaggtt ccggctggac cgtttcgtat tcaggatctt ggtgattctg
     8881 tctccggtac gttgcatatt cgcattgaag aacagaacgg ccaggtgcag gaatatgaca
     8941 tcagcaccgc ctcgatgcca tacctcactc gtccaggtca ggttcgctat aagatcatga
     9001 tgggccgtcc gcaagagtgg ggacaccatg tcgagggtga atttttttct ggtgctgaag
     9061 cttcctgggg gatcgctaac ggctggtcgt tatatggcgg cgcactggga gatgaaaact
     9121 atcagtctgc ggcgcttggc gtcggtcgcg atttgtctac attcggcgcg gtcgcgtttg
     9181 atgttactca ctcgcacacc aaactggata aagacaccgc ttatggcaaa ggttcgctgg
     9241 acggtaactc cttccgtgtg agttattcca aagactttga ccagctcaac agccgcgtta
     9301 cttttgctgg atatcgcttc tcggaagaga actttatgac catgagcgag tatctggatg
     9361 ccagtgacag cggaatggta cgcacgggca acgacaaaga gatgtacact gccacttata
     9421 accagaactt ccgcgatgcg ggtgtttcgg tttatctcaa ctatacccgc catacctact
     9481 gggatcgcga ggagcagaca aactacaaca tcatgctctc gcactatttc aatatgggca
     9541 gtattcgtaa tgtcagcatc tcgatgactg gctaccgtta cgagtatgac aaccaggccg
     9601 acaaaggcat gtacatttcg ctcagtatgc cgtggggtga caacagtacc gttagctata
     9661 acggtaacta tggcagtggg acggacagca gtcaggtcgg ttatttcagc cgtgtcgatg
     9721 acgcgactca ctatcagttg aacgtcggca ccagtgacaa acacaccagc gttgacggct
     9781 attacagcca tgatggttcg ctggcgcagg ttgacctcag tgcgaactac catgaagggc
     9841 aatacacctc tgcgggcttg tcgttacagg gcggcgcaac gcttactgcc cacggtggcg
     9901 cacttcaccg tacccagaat atgggcggga cacgcttgtt gattgatgcc gatggcgttg
     9961 ccgatgttcc ggtagaaggt aacggtgctg ctgtttatac caatatgttt ggtaaagccg
    10021 tcgtttctga cgtcaataac tattaccgca atcaggcgta tatcgacctc aacaaactgc
    10081 cggaaaacgc cgaagcaacc cagtcggtgg tacaagccac gctaactgaa ggggcgattg
    10141 gctaccgcaa atttgccgtc atcagtggtc aaaaagcgat ggcggtgctg cgtttacaag
    10201 acggcagcca tcctccgttt ggcgcagaag taaaaaatga taacgagcag acagtgggcc
    10261 ttgtcgatga tgacggcaat gtttatctgg ctggggtgaa acctggcgaa cacatgagtg
    10321 tgttctggag tggtgttgcg cattgcgata tcaacctgcc ggacccgctg cctgccgatc
    10381 tgtttaacgg cttgttactg ccatgccagc ataaaggcaa cgtcgcgccg gttgttcctg
    10441 atgacataaa gcctgtcatt caggagcaga cgcaacaggt gacacccacc aatccccctg
    10501 tttctgtttc agctaaccaa taacgtgatt aaggaatgat ccatgtctga tttactttgc
    10561 tctgccaaat tgggtgctac gacgctagcg ttactgctta gcgctgccag tttgtcgccc
    10621 aggcttctgt aacgccagat cgtacccgtt tgatatttaa tgaaagcgac aaatcaatca
    10681 gcgtgacgtt gcgcaataac gatccgaaac tgccttatct ggcccaaagc tggatcgaag
    10741 atgaaaaggg taacaagatc agttcaccgt taactgttct gccaccggtg caacgcatcg
    10801 attcaatgat gaacgggcag gtaaaagtgc agggaatgcc ggatatcaac aaattgcctg
    10861 ccgatcgtga aagcctgttc tacttcaacg tgcgtgagat cccgccaaaa tcaaacaaag
    10921 ccaacacgct gcaaatagca ctacaaacgc gtattaagtt gttctggcga ccgaaggcgc
    10981 tggagaacgt cagcatgaaa aacccctggc aatataaggt tactttaacc cgcaacggtc
    11041 aggaatttac cgtcaataac ccgacgccgt attacgtcat tttcagtaat gccagcacac
    11101 agaaaaatgg caatcctgca gccggtttct cgccaatagt gatgtcgccg aaaacctctg
    11161 caccgctgaa tgtgaagatg ggtagcgtgc cggtactgac ctatgtcaac gactatggcg
    11221 ctcggatgcc gctctttttc acttgcaacg gtaacagttg ccaggtcgat gaagagcaga
    11281 gccgcaaagg ctgaggccgg gagagccagt atgaaaggat cttgttctgt ctgggcggta
    11341 actgggttac tgatatgttg tgttggtagc ggctttgcag ttgcagcgag taagtcagta
    11401 agtatgaatt tacgggtgct tgtcgatgcc cctcctcctt gtacggtaaa tggtgcggca
    11461 gttgagtttg gtaatgtatt cattaataag atcaatgggg ttgattataa acgtcccatt
    11521 gactactcgc ttgtttgtaa caatctggca atggatgacc tgcgtctaca gatgcaggcg
    11581 acaacggtcg tgattaacgg tgaaacagtt atcagcacgg gtattcctgg gtttggtatt
    11641 cgggttcaga aaagtagtga tcacacaata ctcgacttaa cctctggctc atggttgcca
    11701 tttaattttt cctcgggagt tcccgttctg gaagctgtgc cggtgaaaca gagtggtaca
    11761 acgttagcgg cagctgaatt taacgccagt gccacaattg tggtggatta ccaatgaaac
    11821 ggatctcgct tattttgctg tggggctttt gctcgatggc actctcaaac gtctcttttc
    11881 acggttattt agtgcagccg cctaactgca cgattagcaa cgctcaaacc attgaaataa
    11941 cttttcagga tgtgcttatt gatgacatta atggcagcaa ttatgagcaa accgtgccgt
    12001 attccattac ctgcgatacg gcggttcgcg acccattaat ggaaatgacc ttgagctgga
    12061 gtggcacact aagcgatttt gataatgctg cagtaagcag caatatcact ggtttaggca
    12121 ttcagctcaa acaggcaggg cagagtttta cgatcaatac gccacttatg gtgaatgaaa
    12181 ccgatttacc ggtgttaacg gctgtgccag taaaaaagag tggtgtcgtt ctgccagagg
    12241 cggactttga ggcctgggcc acgttacagg tagattacca atgaagaaaa agcgtacgtt
    12301 gttttttatt agtagcctga tgttgctggg cagtggaacc acaatagcag gagataattt
    12361 gcactttacc ggcaatttaa tcagtaaatc gtgtacgcca gtgattaatg gtagccagtt
    12421 ggcagaagta cattttcctg ccattgccgc cagcgattta atgaaccttg ggcagtcaga
    12481 gcgagtgcca ttggtgtttc aattgaaaga ttgtcatagt tctacattgt ttaatgtcaa
    12541 agtaacgttg acaggaacag aagatagtgc tctacccggc tttctggcct ttgactcctc
    12601 gtcatcagca tccggcgctg ggattggtat tgaaactgcg gcaggcacat cggtgccaat
    12661 caacaataca acaggcgtca cgctcccgct gaatcaggga aataacagcc taaattttaa
    12721 tacctggtta caggcaaaga gcgggcgaga tgtaacgtcc ggagacttta gtgccacagt
    12781 gacggcaacc tttgagtatt tctgaaatga aaaaatggac cataattttc gcgagcctaa
    12841 tgctgctcgt tctgagtgtt gtcggcgcat ctaagagtta tgctggagat aaacttatga
    12901 gtgcgagttt tgacagtgtg agagtttatt atgccatgga caaggtaacc ggtgctgtag
    12961 cgagcagtgt tttcaatgtt acggtgataa cgccaaagga ggttgcatat gggaaatatg
    13021 attcttttgc gtataaaggc gatacgttgc gggttatttc ctggtctggg tcgggttcag
    13081 ctccaacttt ggtactgact gactttgata caattaataa ctcaaattgc cctggcatag
    13141 atacgaaaat ttttcgttgt gcatatatga cgtttaaaat taccgttgca tctgatgatt
    13201 atggttgccc ttggatcgca tccttttatt cctatacaga tttacccgga tttggaagct
    13261 atacggcccc tacagtgcat aacacaatat gcccaacgat accggtcact tcatacgata
    13321 tctcctggag tgaaaattat gtcagccata ataaggcgtt acgaattcag tccacggggt
    13381 caacagtgac caccacgctt tcgacctacc tgatggaagg cggcagactc tgtgatggca
    13441 gtaatttttc tgataatgat ggcagaggag catactgccg cgcggtatca gaattgttaa
    13501 cctttacctc ttacggttgt gataaatcaa cggtaacggt tacgccaact cgtcatccgg
    13561 taacggataa agtactacac gatattgtgg tgaatgtgaa taccagtagt gggcaaccga
    13621 ttgattcgac gtgtcgtttt cagtatgtat tgaatgaact gtagttcgta gtattgctct
    13681 gatgcgtctt atcaggccta cgaagtgaat tgcaatttat tgaattatca catatttgta
    13741 ggccggataa ggcgtttacg ctgctccggc atttgctgcc agcattcagg gcaactccgg
    13801 cggcaaccac tcttccactt caatcaacac caacaaagcc gcatcaccgc cgtactcttt
    13861 cggtgcctgg tgaaatgcca tcacatgcgg atgttgtgcc agccacagcg gtgtttgttg
    13921 cttcaaaata tgcttcccgt gcccatgcat cacgcaggca caaaacacat gttcgcggcg
    13981 acaggcggca atcaacgcgc ccagttcctg cttggcctgc agctgcgtca gaccgtgtaa
    14041 atccaaaaac aattccggtg aataatcgcc gcggcgcagt ttcttcgcct caaaatggct
    14101 gacatccggg cgaacatatt tcaccggacc ttcggtattt aataacggct gaaactcatc
    14161 ggagaaataa tggctggcat cagcctgctc ctggatcaag cgtttcaccg gcacttcgct
    14221 gattttttta cgctgcggtc ggtggacaat cgtgtcctgc ttaatcttgc gagtccccgc
    14281 catcaactgg cgaaacagag cctggtcctc ctcgctaagt gttgttttct ttttcattta
    14341 tctgtctcta tttctaattt tcctacagtt tacccgactc gtagcacaat cgatcgtttt
    14401 aaacgtattt ttcctcactc accgcgcgtg aatgctgatt tatcgtcatc ttcatggcaa
    14461 actagccgcc gaaattattg cgatcatgcc ctggaggaat acgtggataa aattttcgtt
    14521 gatgaagcag taaatgagct gcaaaccatt caggacatgt tgcgctggtc ggtaagccgc
    14581 ttcagcgcgg caaatatctg gtacggtcac ggcaccgata acccgtggga tgaagccgta
    14641 cagctggtgt taccatcgct ctacctgccg ctggatatcc cggaagatat gcgcaccgcg
    14701 cgtctgacct ccagcgaaaa acaccgtatt gttgaacgcg tgatccgccg cgtcaacgag
    14761 cgcattccgg tggcttacct gaccaacaaa gcctggttct gcggtcatga attttacgtc
    14821 gatgaacgcg tgctggtgcc gcgctcgccg attggtgaac tgatcaacaa taaatttgcc
    14881 ggactcatta gcaagcaacc gcagcatatt ttagatatgt gtaccggtag cggctgcatc
    14941 gccattgcct gtgcttatgc cttcccggaa gcggaagtcg acgcggtaga tatctctccc
    15001 gacgcgctgg cggttgccga acagaacatc gaagaacacg gtctgatcca caacgtcatt
    15061 ccgattcgtt ccgatctgtt ccgtgatttg ccgaaagtgc agtacgatct gattgtcact
    15121 aacccgccgt atgtcgacgc ggaagatatg tccgacctgc caaacgaata ccgtcacgag
    15181 ccggaactgg gcctggcatc tggcaccgac ggccttaaac tgacgcgtcg cattcttgga
    15241 aacgcggcag attaccttgc tgatgatggc gtgttgattt gtgaagtcgg caacagcatg
    15301 gtacatctta tggaacaata tccggatgtt ccgttcacct ggctggagtt tgataacggc
    15361 ggcgatggtg tgtttatgct caccaaagag cagctgctgg cggcccgtga atatttcgcg
    15421 atttataaag attaagtaaa cacgcaaaca caacaataac ggagccgtga tggctggaaa
    15481 cacaattgga caactctttc gcgtaaccac cttcggcgaa tcgcatgggc tggcgctcgg
    15541 ctgcatcgtc gatggtgttc cgccaggcat tccgctgacg gaagcggacc tgcaacatga
    15601 cctcgaccgt cgtcgccctg ggacatcgcg ctataccacc cagcgccgcg agccggatca
    15661 ggtcaaaatt ctctccggtg tttttgaagg cgttactact ggcaccagca ttggcttgtt
    15721 gatcgaaaac actgaccagc gctctcagga ttacagcgca attaaagacg ttttccgccc
    15781 aggccatgct gattacacct acgaacaaaa atacggtctg cgcgattatc gcggcggtgg
    15841 acgttcttcc gcccgcgaaa ccgccatgcg cgtggcggca ggggcgattg ccaaaaaata
    15901 tctcgccgag aaatttggta ttgaaatccg cggctgcttg acccagatgg gcgacattcc
    15961 gctggaaatc aaagactggt cgctggttga acaaaatccg ttcttctgcc cggacccgga
    16021 caaaatcgac gcgttagatg aactgatgcg cgcgctgaaa aaagagggcg actccatcgg
    16081 cgcgaaagtc accgttgttg ccagtggtgt ccccgccgga cttggcgagc cggtctttga
    16141 ccgcctggat gccgacatcg cccatgcgct gatgagcatc aacgcggtga aaggcgtgga
    16201 aattggcgac ggttttgacg tagtggcgct gcgcggcagc cagaaccgcg acgaaatcac
    16261 caaagacggt ttccagagca accatgcggg cggcattctc ggcggtatca gcagcgggca
    16321 gcaaatcatt gctcatatgg cgctgaaacc gacctccagc attaccgtgc cggggcgcac
    16381 tattaaccgc tttggcgaag aagttgagat gatcaccaaa ggccgtcacg atccctgtgt
    16441 cgggatccgt gcagtgccga tcgctgaagc gatgctggcg atcgttttaa tggatcacct
    16501 gttacggcag cgggcgcaaa atgccgatgt gaagactgat attccacgct ggtaaaaaat
    16561 gaacaaaacc gcgattgcgc tgctggctct gcttgccagt agcgctagcc tggcagcgac
    16621 gccgtggcaa aaaataaccc aacctgtgcc gggtagcgca caatcgatag gcagtttttc
    16681 taatggctgt attgtcggcg ctgacacgtt gccgatacag tccgaacatt atcaggtcat
    16741 gcgtaccgat cagcgtcgct atttcggtca cccggatctg gtgatgttta tccagcgtct
    16801 gagtagccag gtgagcaatc tgggcatggg tacggtgctg attggcgata tggggatgcc
    16861 cgctggtggg cgtttcaacg gcggtcatgc cagccaccag accggactgg atgtcgatat
    16921 ctttctgcaa ctgccgaaaa ctcgctggac ctccgcgcag ctcttgcgcc cgcaagcact
    16981 ggacttagtt tcccgcgacg gtaaacacgt tgtctccacg ctgtggaagc cagaaatttt
    17041 cagcttgatc aaactcgctg cccaggacaa agacgtcacg cgcatttttg ttaatccggc
    17101 gattaaacaa caactttgcc ttgatgcggg caccgatcgc gactggttgc gcaaagtgcg
    17161 accctggttc cagcatcgcg cgcatatgca tgtacgatta cgttgccctg ccgatagtct
    17221 ggagtgtgaa gatcaacctt taccgccatc aggcgatggt tgcggggcag aactgcaaag
    17281 ctggtttgca cctctaaaac cgggaacaac aaagcctgag aagaagacac cgcctccgtt
    17341 gccgccttcc tgccaggcgc tactggatga gcacgtgatc taatggaaac gtttaatagc
    17401 ctgtttatgg tttccccgct gttgctggga gttctctttt ttgtcgccat gctggcggga
    17461 tttatcgact cgattgccgg tggcggtggg ttactcacca ttccggcatt gatggctgcg
    17521 ggaatgtctc ccgctaacgc gctggcaacc aataaactgc aagcctgcgg cggctctatt
    17581 tccgccacta tctactttat tcgccgcaaa gtggttagtt taagcgatca gaaactcaat
    17641 attgccatga cctttgtcgg ctcaatgagc ggcgcactgt tggtacagta cgttcaggct
    17701 gatgtcttac ggcagatttt gcccattctg gtgatttgta tcggcctcta tttcttgctg
    17761 atgccaaagc tgggtgaaga agatcgccag cgccggatgt acggtttacc cttcgcgtta
    17821 attgccggtg gctgcgtcgg tttttatgac ggattctttg gcccggctgc cggatcgttt
    17881 tacgcgctgg ccttcgttac gctgtgcgga ttcaacctcg ccaaagccac ggctcacgcc
    17941 aaattactca acgcaacgtc aaacattggc ggtctgctgc tgtttattct cggcggcaaa
    18001 gtgatttggg cgacgggctt tgtcatgctg gtggggcagt tcctcggcgc gcgcatgggt
    18061 tcacggctgg tgttgagcaa aggacaaaag ctgatccgcc cgatgattgt tatcgtctcg
    18121 gcggtgatga gtgccaaatt actttacgat agccacggac aggagatcct ccactggttg
    18181 gggatgaact aatgaacagt acacaccact atgagcagtt gattgaaatt tttaatagct
    18241 gctttgccga tgaatttaat acccgtctga ttaaaggcga cgacgaaccg atctatcttc
    18301 ctgctgatgc ggaagtaccg tataaccgaa ttgtctttgc ccacggcttt tatgccagcg
    18361 caattcatga gatttcgcac tggtgtattg ccggaaaagc gcgccgtgag ctggttgatt
    18421 tcggctactg gtattgcccg gatgggcgtg atgcccaaac gcaaagccag tttgaagatg
    18481 ttgaagtgaa gccgcaggca ctcgactggt tgttctgtgt ggcggcggga tatccgttta
    18541 acgtcagttg cgacaatctg gaaggcgact tcgaaccgga tcgtgttgtg tttcaacggc
    18601 gggtacatgc gcaggtgatg gattatctga caaatggcat tcccgagcgt ccggcacgct
    18661 tcattaaagc actacaaaat tattatcaca cgccggaact tacggcggaa cagttcccgt
    18721 ggccggaagc gctcaactga agccggtgcc acgttttaca atgaggaaag aagatgatcg
    18781 cggagtttga atcacgcatt ctggcattaa tcgacggcat ggttgaccat gccagtgatg
    18841 atgagttgtt tgccagtggg tatttgcgtg gccacctgac attagccatc gcagaactgg
    18901 aaagtggtga tgaccactcc gctcaggcgg tgcatacgac cgttagccag agtctggaaa
    18961 aagccattgg tgccggtgaa ttgtcgccgc gtgaccaggc gctggtgacc gatatgtggg
    19021 aaaacctgtt tcagcaggcg tcacagcagt aagttgcggc gtctggagca ccatgcctga
    19081 tgcgacgctt atcgcgtctt atcaggccta cacgtcacac gtaggtcgga taaggcgttc
    19141 acgccgcatc cggcaaacac tgcgagagca gattaccccg ccttaaccgc tttacccttc
    19201 aacaatttcc gcacccataa ccgattcggg tttaacgccg tcagcgtact ggcatccatc
    19261 ggaatcggtt cgtcgctcat ctgcgccgcc agaatctcgg cacacagcgg agcagaacac
    19321 aaaccgcgag aacctaaagc cgcaaacata aagagatcgt caaaaaccgg cgcgcttacc
    19381 gcctcatctt tctgctccgc caacgacgca tattccacga gtgttgcctc ataatcggga
    19441 acattgccta ccattggcag atgatcgcgg gtggcacaac gcacaccgca gcgcgcctct
    19501 ttatcactga catcaacctc ttttgcccac tgtgcctgcg ggaaacaatc aatcaaccgc
    19561 tggcgattct gctgctgatc gtcctcactg tacgccgtat cttcgctgcc gcgatgataa
    19621 ctggcaccaa tacaatgatg ttgattcgcc ggattttgtg gcgtgagata accgtcatag
    19681 cacagcacct gcttcagctc tgccagttcc ggcgttgtcg gaatatggct gacctgcccg
    19741 gcaaccgaat acaccgggag agtcgacgtt tggctgaaac ggctgatttg atgcccgttc
    19801 gccagtacca ctacgctgtg tgttgcttgc tgatctcctg caaaattcaa caaccaacag
    19861 tcatccttac gggataaatt ctgtaactga tattgataat aaatctgcaa accctgctgt
    19921 tgcgccagtt ccagcacatt acgggtcagt tctgctgggc acagccaacc accttgcgga
    19981 taagtaatgc cgctgcaatt tgtcgcaacg cccgtaattt gttcaaccgc atttgcctca
    20041 acggctacag ccagttctgc gggtaaatcc attgacaaca tctgtgcgat tttatgctgg
    20101 cttttctcat cccagcctaa ctgcgtgacg ccgcaccagt catgatcaaa tttaacgggt
    20161 aattggtcgt aaaaccgacg agcaaaagta aacgcattag agaaaaagcg gtttagcgcc
    20221 tcatcgtgtt tgcttaataa cggatacagc gccccctggc gattgccgga agcacccagc
    20281 gcgggggctt catccgcgca ataaagcgct acctgccagc cgcgccgtaa taacgccagc
    20341 gacaacaacg cgctggcaat accaccgccg ataatcgcta cttcccgttt gctgctgccc
    20401 gtacgattaa accacggcgt ggagcagggg agtggtaatg tctgttccat caccccacaa
    20461 agcatttccc gtttgcgccc aaagccctta cgtttttgca tcgtgaatcc ggcttcctgc
    20521 aaaccgcggc ggacaaaacc ggcagacgta aatgtcgcca gcgtgccgcc tggacgcgcc
    20581 aaccttgcca tggcgttaaa cagattttgc gtccacatat ccgggttttt agctggcgca
    20641 aagccgtcca gaaaccaggc atctactttt tgatttagcg aatcgtccag ttggctggtc
    20701 agttcgttaa tatcgccaaa ccataaatcc agcgtcacgc ggccttcatc gagcaataaa
    20761 cgatggcaac cgggcaaggg cattggccac tgcgcctgaa gttgttctgc ccacggagcc
    20821 agttccggcc agtgttgatg cgctaaggct aaatccgcac gggtgagggg aaatttctca
    20881 aaactaatga aatgtaagcg ttgtaattgc gcttgcggat gcgcttcgcg aaactgatca
    20941 aatgcctgcc atagcgtcag gaagtttaat ccggtgccga agccgctctc tgctaccaca
    21001 aacagaggat gtggatgctc aggaaagcgt acctctaatt ggttgcctcc cagaaaaaca
    21061 taacgcgtct cttccagccc gttatcgttg gaaaaataga catcgtcaaa atctcgggaa
    21121 acaggtgtac cctcagcatt aaattcgagg ttggcaggtt gtatggagta gtgtttcacg
    21181 taagttactc gtcttacagg cggtggctcg atcttagcga tgtgtgtaag gctgcgcaaa
    21241 tttctctatt aaatggctga tcggacttgt tcggcgtaca agtgtacgct attgtgcatt
    21301 cgaaacttac tctatgtgcg acttacagag gtattgaatg aaacgtgcag tgattactgg
    21361 cctgggcatt gtttccagca tcggtaataa ccagcaggaa gtcctggcat ctctgcgtga
    21421 aggacgttca gggatcactt tctctcagga gctgaaggat tccggcatgc gtagccacgt
    21481 ctggggcaac gtaaaactgg ataccactgg cctcattgac cgcaaagttg tgcgctttat
    21541 gagcgacgca tccatttatg cattcctttc tatggagcag gcaatcgcag atgcgggcct
    21601 ttctccggaa gcttaccaga ataacccgcg cgttggcctg attgcaggtt ctggcggcgg
    21661 ttccccgcgt ttccaggtat tcggcgctga cgcaatgcgc ggcccgcgcg gcctgaaagc
    21721 ggttggcccg tatgtggtca ccaaagcgat ggcatccggc gtttctgcct gcctcgccac
    21781 gccgtttaaa attcatggcg ttaactactc catcagctcc gcgtgtgcga cttccgcaca
    21841 ctgtatcggt aacgcagtag agcagatcca actgggcaaa caggacatcg tgtttgctgg
    21901 cggcggcgaa gagctgtgct gggaaatggc ctgcgaattc gacgcgatgg gtgcgctgtc
    21961 tactaaatac aacgacaccc cggaaaaagc ctcccgtact tacgacgctc accgtgacgg
    22021 tttcgttatc gctggcggcg gcggtatggt agtggttgaa gagctggaac acgcgctggc
    22081 gcgtggtgct cacatctatg ctgaaatcgt tggctacggc gcaacctctg atggtgcaga
    22141 catggttgct ccgtctggcg aaggcgcagt acgttgtatg cagatggcga tgcatggcgt
    22201 tgatacccca atcgattacc tgaactctca cggtacttcg actccggttg gcgacgtgaa
    22261 agagctggca gctatccgtg aagtgttcgg cgataagagc ccggcgattt ctgcaaccaa
    22321 agccatgacc ggtcactcac tgggcgctgc gggtgtgcag gaagctatct actctctgct
    22381 gatgctggaa cacggcttta tcgccccgag catcaacatt gaagagctgg acgagcaggc
    22441 tgcgggtctg aacatcgtga ccgaaacgac cgatcgcgaa ctgaccactg ttatgtctaa
    22501 cagcttcggc ttcggcggca ccaacgccac gctggtaatg cgcaagctga aagattaatt
    22561 cgcagtaggc cggagtaggt cggataagac gcgccagcgt cgcatccgac gttacgcgcc
    22621 aatgcggcct ccggcactaa cgcaaaaggg aacctgatgg ttcccttttt cacatcattg
    22681 acaatcgcca ccagttccag gcaaacttcc cgctttgtcg atttccttct gaaaagacgt
    22741 acgcgttaaa tcctgccaac gcaccgtaac cctgaaacca gagagatgag acggggatac
    22801 tcctcgcctt gcgctgcatt ctggagtaat gcatgactgc tgtaagccaa accgaaacac
    22861 gatcttctgc caatttttcg ctcttccgca tcgcttttgc ggtttttctc acctacatga
    22921 ccgtagggct gccgttgccg gttatcccgc tgtttgttca tcatgaactg ggctatggca
    22981 ataccatggt cggcattgcc gtcgggattc agtttctggc tacggtgctg acgcgtggct
    23041 atgccgggcg actggccgat caatatggcg caaaacgttc ggcgcttcag gggatgttag
    23101 cttgtggtct ggctggcggc gcgttgctgc tggcggcgat tttgcctgtc tccgcaccgt
    23161 tcaaatttgc cctgttggtc atcgggcgtt tgattcttgg gtttggtgaa agccagttac
    23221 tgacaggcgc tctgacctgg gggttaggca tcgtagggcc aaaacactct ggcaaagtga
    23281 tgtcatggaa tggaatggcg atttacggtg ccctcgctgt tggtgctccg cttggcctgt
    23341 tgattcatag ccattacggt tttgccgcac tggcgatcac cacaatggta ttacccttac
    23401 tggcgtgggc ctgtaacggc acagtgcgca aagtaccggc cctggcggga gaacgtccat
    23461 cgctgtggag cgttgtcggg tttatctgga aaccagggtt agggctggca ctacaaggcg
    23521 ttggttttgc ggttatcggg actttcgttt cgctctactt tgccagcaaa ggatgggcga
    23581 tggcgggctt tactcttacc gagtttggcg gcgcatttgt cgtgatgcgc gtcatgtttg
    23641 gctggatgcc ggaccgtttt ggcggcgtga aagtggcgat tgtctctctg cttgtagaaa
    23701 cggtgggctt gttgctgctc tggcaagccc caggtgcgtg ggtcgcatta gcgggcgcgg
    23761 cgttaaccgg agccggatgt tcgcttatct ttcctgcgct gggcgtggag gtggttaaac
    23821 gcgtcccctc acaagttcgc ggcaccgcac tgggcggtta cgccgcgttt caggatatcg
    23881 ccctcggcgt ctccgggccg ctggcgggaa tgctggcgac cacgtttggt tactcttcgg
    23941 tatttcttgc cggggcgatc tctgcggtgc tgggaattat tgtcacgata ctgtcatttc
    24001 gtcggggcta acgaaccagc cagaccagca tcaggataat cgcgactaac agtatccaca
    24061 gcgcgggacg cgttgccata ttttgcaacg tgtccatcat cggtggaaaa ggattaagca
    24121 gcggttgcat atcacgcgga tcaatccctt taacccgccg ttgataaagc tgattaagaa
    24181 tatcctcggc ctgggcagaa gaaagcgatg attgcgcaga aagaccatat ttttgctgga
    24241 tatacgccga taacgctgcc agttcactgg catctaaagg ttgttttagc gccatctgta
    24301 gtgattccag cgtcggcgta ttttgctggc ttaacgtctg gcgcgcctgc aaccaggtca
    24361 ccagatggtt aaacagtttc gctggaatta actcgccatc gttcaccccg gaaagttcca
    24421 gcatcgattg ccagatctgt ttgctgggtt cccccgttgc cgccgcaagt ttggtcacca
    24481 gctgttttag cgcattgtgc tccgccggta ataaaggacg gtcggtcgcc tcgcgctgct
    24541 gtggttgcgg aataaccatc ttcccttcct gcaacagggt gagaatggtt tttaattgct
    24601 ccggtgagag ctgattcagc ggcgtctgac caaagttatg acggatataa tccgtgaccg
    24661 cctgacgatt attccccaga cgtaaatact cccctaattg cgctaaaagc tggcgggcag
    24721 aatggctttt ttgcgcggcc agcagacgtt gcgccagatt atgctcagcg gcagggaagt
    24781 gacgcgaaag cagcggtgaa tctcccgaca gaccaatatc gtgcctgata cccgcccaca
    24841 gttctgctct ttgttgttgc gtcagtgagg tcactttcgt cattaagctt tccagcgaag
    24901 tacgttgctg actggataaa ggctgattgc ccgcgccaga cggcaggtta tctccctgac
    24961 ctggtggttg cccaggagga gggccggaaa taggttgtat cattacgtat ccttatacct
    25021 gaaatcttcg caagtatgcc tggccgcgag attatggcac acttgtccgg ttaactctcg
    25081 tctcatacag gtaacacaaa cgtgaaaatc cttgttgatg aaaatatgcc ttatgcccgc
    25141 gacttattta gccgtttggg tgaggtgacc gcggttcccg ggcgtccaat ccccgtcgct
    25201 caactggcag acgcggatgc gctgatggtg cgttcggtca cgaaagtgaa tgaatctttg
    25261 ctggcaggaa aacccattaa atttgttggc actgccactg cggggaccga ccatgtcgat
    25321 gaagcatggt tgaagcaggc gggaattggt ttttccgctg cacctggctg taatgcgatt
    25381 gcggtggtgg aatatgtttt ctcctcacta ctgatgcttg ccgaacgcga tggattttca
    25441 ctgcacgacc gtacggtggg gatcgtgggc gttggtaacg ttggacgtcg attgcaggcg
    25501 cgactggaag cgttagggat taaaacattg ctttgcgatc cgcctcgcgc tgaccgtggg
    25561 gatgagggtg atttccgctc gctggatgaa ttagttcagc atgcggatat tctgactttc
    25621 catacgccac tcttcaaaga tgggccgtac aaaacgctgc atctggcgga tgaaaaactg
    25681 atccgtagcc tgaagccggg agcgattctg attaacgcct gccgtggcgc agtcgtcgat
    25741 aatactgcgt tgctgacctg cctgaatgaa ggccagaagt taagcgtagt gctggatgtc
    25801 tgggaaggcg aaccggaact taacgtggag ttgctgacaa aagtggatat cggcacgccg
    25861 catatcgcag gttataccct ggaaggtaaa gcacgcggta ctacgcaagt gtttgaagcc
    25921 tatagcaagt ttattgggca tgaacagcac gttgcgctgg atacattact gcccgcgcca
    25981 gagtttggtc gcattacgct gcatggcccg ctcgatcaac cgacgctgaa aaggctggtg
    26041 catttggtgt atgatgtgcg ccgcgatgac gcaccgctgc gtaaagtcgc cgggataccg
    26101 ggtgagttcg ataaactgcg caaaaactat cttgagcgcc gtgaatggtc atctctgtat
    26161 gtaatttgtg atgacgccag tgcggcatca ttgctgtgta aactgggttt taacgccgtt
    26221 catcatccgg cacgttaatc tcttcttcat gctctctgct gtaacattgg cagggagctt
    26281 tgctatttct ggagtaaacc accatgtctg aaggctggaa cattgccgtc ctgggcgcaa
    26341 ctggcgctgt gggcgaagcc ctgcttgaaa cgctggctga acgtcagttc ccggttgggg
    26401 aaatttatgc actggcacgt aacgaaagcg caggcgaaca actgcgcttt ggtggtaaaa
    26461 caatcactgt gcaggatgcc gctgaattcg actggacgca ggcgcagctg gcattttttg
    26521 tcgcaggcaa agaagctacc gctgcctggg ttgaagaagc gaccaactca ggttgcctgg
    26581 tgatcgacag cagcggactg tttgctctcg aacccgacgt accgctggtg gtgccggaag
    26641 taaacccgtt tgtactgacc gattaccgca accggaatgt catcgccgta ccagacagtc
    26701 tgaccagcca gctgctggcg gcactgaaac cgttaatcga tcagggcggt ttgtcgcgta
    26761 tcagtgttac cagcctgatt tcagcctccg cccagggcaa aaaagcggtc gatgcgttag
    26821 cggggcagag tgcgaaattg ctcaacggca ttccgattga cgaagaagat ttcttcggtc
    26881 gtcaactggc gttcaatatg ctgccgttgt tgccggatag cgaaggtagc gtgcgtgaag
    26941 aacgtcgtat cgttgacgaa gtacgcaaaa tcctgcagga cgaagggctg atgatttcgg
    27001 caagcgtcgt ccaggcaccg gtattctacg gtcatgccca gatggtcaac tttgaagcac
    27061 tgcgtccgct ggcggcagaa gaagcgcgtg atgcgtttgc tcagggcgaa gatattgtgc
    27121 tctctgaaga gaacgaattc ccgactcagg taggggatgc ttcgggtacg ccgcatcttt
    27181 ctgttggctg cgtgcgtaat gactacggta tgccggagca agtccagttc tggtcggtgg
    27241 ccgataacgt tcgctttggc ggcgcgctga tggcagtaaa aatcgccgag aaactggtgc
    27301 aggagtatct gtactaatgt ccgaccagca acaactgcca gtttataaaa ttgcgctggg
    27361 cattgagtac gacggcagta agtattacgg ctggcaacgg cagaatgaag tccgcagtgt
    27421 gcaggagaag ctggaaaagg cgctctccca ggtggcgaac gaacccatca ccgtcttctg
    27481 cgccgggcgt actgacgcag gggtacacgg taccgggcag gtggtgcatt tcgaaacaac
    27541 cgcgcagcgc aaagacgcgg cgtggacctt gggcgtaaat gcgaatttac ctggtgacat
    27601 cgccgtgcgt tgggttaaag ctgtacctga tgattttcat gcccgattta gcgccacggc
    27661 tcgccgttat cgctacatca tctacaatca tcggctgcgc ccggcggtat tgagtaaagg
    27721 ggtaacccat ttttacgaac cgctggacgc tgaacggatg catcgggctg cgcaatgctt
    27781 gctgggcgag aatgatttca cctcgttccg tgcggtgcag tgccagtccc gaaccccgtg
    27841 gcgcaacgtt atgcatatta atgtcacgcg tcacggtcct tatgtggtgg tagatatcaa
    27901 agcgaatgcc tttgtacatc atatggtcag gaatattgtc ggcagcctga tggaagtcgg
    27961 tgcccacaac cagccggaga gctggatagc agagttgctg gcggcaaagg acagaacgct
    28021 ggcggcagca acggcaaaag cggaagggct gtatctggtc gcggtggatt accctgaccg
    28081 gtatgatctt ccaaaaccgc caatgggccc gctatttctg gcggactaac gagaataatg
    28141 ctcggaacat ttcaggctgt agccaacgta gttacagcct gaaagatgac gagtacaagg
    28201 cataggcaaa tatggacctg atttatttcc tcatcgattt tatcctgcac attgacgtgc
    28261 atctggcaga gctggtcgcg gagtacggcg tctgggttta tgccattctg tttttgattc
    28321 tgttctgcga aacggggctg gtggtaacgc cattcctgcc tggtgattcg ctgctgtttg
    28381 ttgctggtgc attggcgtcg ctggaaacta acgatctcaa tgtccatatg atggtggtgc
    28441 ttatgttgat tgccgctatt gtcggtgatg cagtgaacta taccattgga cggttatttg
    28501 gtgacaaact gttcagtaac ccggattcga aaatctttcg tcgcagttat cttgataaga
    28561 ctcatcagtt ttacgagaga catggcggca aaacgattat tctcgcccgt tttgtaccga
    28621 tcgtcagaac gttcgcgccg tttgttgcgg gaatgggcca catgtcgtac cgtcatttcg
    28681 ccgcttataa cgtgatcggc gcactgttgt gggtactgat ttttacctac gcgggttatt
    28741 tcttcggcac gctccccttc attcagagca accttaagct gatgattgtt ggcattattt
    28801 ttgtttcaat attgcctggg gttttcgaat ttattcgtca caaacgcgct gcggcacgcg
    28861 ccgcaaaata aaaagtaact ccgcggttcg accacttttt tatccaaagt ttcgggctgt
    28921 tatgttttaa tgtgcaacat tcatggtctg ttgggggcaa aaatggcatt atgcgtcccc
    28981 aaagataaaa ctggcatcga accaggttca gacagaaagg tccctaatga gctggattga
    29041 acgaattaaa agcaacatta ctcccacccg caaggcgagc attcctgaag gggtgtggac
    29101 taagtgtgat agctgcggtc aggttttata ccgcgctgag ctggaacgta atcttgaggt
    29161 ctgtccgaag tgtgaccatc acatgcgtat gacagcgcgt aatcgcctgc atagcctgtt
    29221 agatgaagga agccttgtgg agctgggtag cgagcttgag ccgaaagatg tgctgaagtt
    29281 tcgtgactcc aagaagtata aagaccgtct ggcttctgcg cagaaagaaa ccggcgaaaa
    29341 agatgcgctg gtggtgatga aaggcactct gtatggaatg ccggttgtcg ctgcggcatt
    29401 cgagttcgcc tttatgggcg gttcaatggg gtctgttgtg ggtgcacgtt tcgtgcgtgc
    29461 cgttgagcag gcgctggaag ataactgccc gctgatctgc ttctccgcct ctggtggcgc
    29521 acgtatgcag gaagcactga tgtcgctgat gcagatggcg aaaacctctg cggcactggc
    29581 aaaaatgcag gagcgcggct tgccgtacat ctccgtgctg accgacccga cgatgggcgg
    29641 tgtttctgca agtttcgcca tgctgggcga tctcaacatc gctgaaccga aagcgttaat
    29701 tggctttgcc ggtccgcgtg ttatcgaaca gaccgttcgc gaaaaactgc cgccaggatt
    29761 ccagcgcagt gaattcctga tcgagaaagg cgccatcgac atgatcgtcc atcgtccgga
    29821 aatgcgcctg aaactggcga gcattctggc gaagttgatg aatctgccag cgccgaatcc
    29881 tgaagcgccg cgtgaaggcg tagtggtacc cccggtaccg gatcaggaac ctgaggcctg
    29941 ataactgata agggcagggc cactggctct gcccttttgc tattctcacc gtaacgaatc
    30001 agcggatacc atgattatca aacgcactcc tcaagccgcg tcgcctctgg cttcgtggct
    30061 ttcttatctg gaaaacctgc acagtaaaac tatcgatctc ggccttgagc gcgtgagcca
    30121 ggtcgcggcg cgtcttggcg tcctgaaacc agcgccattt gtgtttaccg ttgcgggtac
    30181 gaatggcaaa ggcaccacct gccgtacgct ggagtcgatt ctgatggcgg cagggtacaa
    30241 agtgggcgtc tacagttcac cgcatctggt gcgttatacc gagcgcgtac gtgtgcaggg
    30301 gcaggaattg ccggaatcgg cccacaccgc ctcttttgcg gagattgaat cggcacgcgg
    30361 tgatatttcc ctgacctatt tcgagtacgg tacgctgtcg gcgttgtggc tgttcaaaca
    30421 ggcacaactt gacgtagtga ttctggaagt agggctgggc ggtcgtctgg acgcaaccaa
    30481 tattgtcgat gccgatgtgg ctgtagtaac cagcattgcg ctggatcata ccgactggct
    30541 gggaccagat cgcgaaagta ttggtcgcga gaaagcaggc atctttcgca gcgcaaaacc
    30601 ggcaattgtc ggtgagccgg aaatgccttc taccattgct gatgtggcgc aggaaaaagg
    30661 tgcactgtta caacgtcggg gcgttgagtg gaactattcc gtcaccgatc atgactgggc
    30721 gtttagcgat gctcacggca cgctggaaaa tctgccgttg ccgcttgtcc cgcaaccgaa
    30781 tgccgcaaca gcgctggcgg cactgcgtgc cagcgggctg gaagtcagtg aaaatgccat
    30841 tcgcgacggg attgccagcg caattttgcc gggacgtttc cagattgtga gcgagtcgcc
    30901 acgcgttatt tttgatgtcg cgcataatcc acatgcggcg gaatatctca ccgggcgtat
    30961 gaaagcgcta ccgaaaaacg ggcgcgtgct ggcggttatc ggtatgctac atgataaaga
    31021 tattgccgga actctggcct ggttgaaaag cgtggttgat gactggtatt gtgcgccact
    31081 ggaagggccg cgcggtgcca cggcagaaca actgcttgag catttgggta acggcaaatc
    31141 atttgatagc gttgcgcagg catgggatgc cgcaatggcg gacgctaaag cggaagacac
    31201 cgtgctggtg tgtggttctt tccacacggt cgcacatgtc atggaagtga ttgacgcgag
    31261 gagaagcggt ggcaagtaag tttcagaatc ggttagtggg cacgatcgtg ctggtggcgc
    31321 tgggggtgat tgtacttcca gggctgctgg acgggcagaa aaaacattat caggatgagt
    31381 ttgcggctat cccgctggtt ccaaaagcgg gcgatcgcga tgagcctgat atgatgccag
    31441 ccgccaccca ggcgttgccg acgcagccgc cggaaggcgc agcggaagag gtgcgggcag
    31501 gtgatgccgc agcaccgtcg ctcgatccgg ccactattgc agccaataac accgagtttg
    31561 aaccggaacc tgcaccggtc gccccaccga agccgaaacc ggtggagccg cctaaaccga
    31621 aggttgaagc accacctgcg ccgaagccag aaccgaagcc ggtcgtggaa gaaaaagcag
    31681 cgccaacggg taaagcctat gttgtgcaac tgggtgcgct gaaaaatgcc gataaagtga
    31741 atgagattgt cggtaagctg cgcggtgccg gttatcgggt ttatacgtcg ccatccacgc
    31801 cagtgcaggg taaaattacc cgtattctgg ttgggccgga tgcctcgaaa gataagctga
    31861 aaggttcgct gggtgagttg aagcaacttt ctggcttaag tggcgtggta atgggctata
    31921 cgccgaatta atacagtctt gcctgatgcg acgctggcgc gtcttatcag gcctgggcag
    31981 tgttagaacc gtaggtcgga taaggcgttt acgccgcatc cgacacgcat tgcacgatgc
    32041 cgcaaaggca taaaaagtcg atggcgttga atattttttc agcgccattt ttattgatgc
    32101 gtgggaagga aatccctacg caaacgtttt ctttttctgt tagaatgcgc cccgaacagg
    32161 atgacagggc gtaaaatcgt gggacacata tggtctggat tgattacgcc ataatcgcgg
    32221 tgattgcttt ttcctctctg gttagcctga tccgcggctt tgttcgtgaa gcgttatcgc
    32281 tggtgacatg gggttgtgct ttctttgttg ccagtcatta ctacacttac ctgtcagtct
    32341 ggtttacggg ctttgaagac gaactggttc gaaatggaat tgccatcgcg gtactgttta
    32401 tcgctaccct gattgttggt gctatcgtga acttcgtgat aggccagttg gtggagaaaa
    32461 cggggttgtc aggcaccgat cgggtgctgg gcgtctgttt cggtgcgttg cgcggtgtgt
    32521 tgattgttgc cgccattctc ttctttctcg actcctttac cggggtgtcg aaaagcgaag
    32581 actggagcaa atcacagctg atcccgcagt tcagttttat catcagatgg ttttttgatt
    32641 atctgcaaag ctcgtcaagt ttcttgccca gagcgtaagt gctctgagat gtggcttaac
    32701 gaggaaaaag acgtatgtgc ggtattgtcg gtatcgccgg tgttatgccg gttaaccagt
    32761 cgatttatga tgccttaacg gtgcttcagc atcgcggtca ggatgccgcc ggcatcatca
    32821 ccatagatgc caataactgc ttccgtttgc gtaaagcgaa cgggctggtg agcgatgtat
    32881 ttgaagctcg ccatatgcag cgtttgcagg gcaatatggg cattggtcat gtgcgttacc
    32941 ctacggctgg cagctccagc gcctctgaag cgcagccgtt ttacgttaac tccccgtatg
    33001 gcattacgct tgcccacaac ggcaatctga ccaacgctca cgagttgcgt aaaaaactgt
    33061 ttgaagaaaa acgccgccac atcaacacca cttccgactc ggaaattctg cttaatatct
    33121 tcgctagcga gctggacaac ttccgccact acccgctgga agccgacaat attttcgctg
    33181 ccattgctgc cacaaaccgc ttaatccgcg gcgcatatgc ctgtgtggcg atgattatcg
    33241 gccacggtat ggttgctttc cgcgatccaa acgggattcg tccgctggta ctgggaaaac
    33301 gtgatattga cgagaaccgt acagaatata tggtcgcttc cgaaagcgta gcgctcgata
    33361 cgctgggctt tgatttcctg cgtgacgtcg cgccgggcga agcgatttac atcactgaag
    33421 aagggcagtt gtttacccgt caatgtgctg acaatccggt cagcaatccg tgcctgtttg
    33481 agtatgtata ctttgctcgc ccggactcgt tcatcgacaa aatttccgtt tacagcgcgc
    33541 gtgtgaatat gggcacgaaa ctgggcgaga aaattgcccg cgaatgggaa gatctggata
    33601 tcgacgtggt gatcccgatc ccagaaacct cgtgtgatat cgcgctggaa attgctcgta
    33661 ttctgggcaa accgtaccgc cagggcttcg ttaaaaaccg ctatgttggc cgcaccttta
    33721 tcatgccggg ccagcagctg cgtcgtaagt ccgtgcgccg taaactgaat gccaaccgcg
    33781 ccgagttccg cgataaaaac gtcctgctgg tcgacgactc catcgtccgt ggcaccactt
    33841 ctgagcagat tatcgagatg gcacgcgaag ccggagcgaa gaaagtgtac ctcgcttctg
    33901 cggcaccgga aattcgcttc ccgaacgttt atggtattga tatgccgagc gccacggaac
    33961 tgatcgctca cggtcgcgaa gttgatgaaa ttcgccagat catcggtgct gacgggttga
    34021 ttttccagga tctgaacgat ctgatcgacg ccgttcgcgc tgaaaatccg gatatccagc
    34081 agtttgaatg ctcggtgttc aacggcgtct acgtcaccaa agatgttgat cagggctacc
    34141 tcgatttcct cgatacgtta cgtaatgatg acgccaaagc agtgcaacgt cagaacgaag
    34201 tggaaaatct cgaaatgcat aacgaaggat gatgccttcg ctgagggtgc cggtctggca
    34261 ccctgacttg caactcccgc cgaaatactg caaagtctgc ctgcaagtct gacagggcaa
    34321 ctatttatga aacgactcat tgtaggcatc agcggtgcca gcggcgcgat ttatggcgtg
    34381 cgcttattac aggttctgcg cgatgtcaca gatatcgaaa cgcatctggt gatgagccag
    34441 gcggcgcgcc agaccttatc cctcgaaacg gatttttccc tgcgcgaagt gcaggcatta
    34501 gctgatgtca cgcacgatgc gcgcgatatt tccgccagca tctcttccgg ttctttccag
    34561 acgctgggga tggtgatttt accctgttca atcaaaaccc tttccggcat tgtccatagc
    34621 tatactgatg gcttactgac ccgtgcggca gatgtggtgc tgaaagagcg tcgcccgttg
    34681 gtgctctgcg tgcgtgaaac accattgcac ttaggccatc tgcgtttaat gactcaggcg
    34741 gcagaaatcg gtgcggtgat tatgcctccc gttccggcgt tttatcatcg cccgcaatcc
    34801 cttgatgatg tgataaatca gacggttaat cgtgttcttg accagtttgc aataaccctt
    34861 cctgaagatc tctttgcccg ctggcagggc gcataactcc gctgttgccc tgtttcaggg
    34921 caattttgca accgcgatca aatcctcgac attttgtttc tgccattcaa tcgaaacgct
    34981 gcgattcaac cgctatacct gctatcttca acttcaggac aataatgcaa cgtcttatta
    35041 acatatttaa cgttgaatgt tactgttgtc gtcaagatgg cataagacct gcatgaaaga
    35101 gcctgcaaac acacaacaca atacacaaca taaaaaagcc attttcactt gagggttatg
    35161 tatgaagaag tcgattctcg ctctgtcttt gttagtcggt ctctccgcag cggcttccag
    35221 ctatgcggcg ctaccggaga cggtacgtat cggaaccgat accacctatg cgccgttctc
    35281 gtcgaaagat gctaaaggtg attttgttgg ctttgatatc gatctcggta acgagatgtg
    35341 caaacggatg caggtgaaat gtacctgggt tgccagtgac tttgacgcgc tgatcccctc
    35401 actgaaagcg aaaaaaatcg acgccattat ttcgtcgctt tccattaccg ataaacgtca
    35461 gcaggagatt gccttctccg acaagctgta cgccgcagat tctcgtttga ttgctgccaa
    35521 aggttcaccg attcagccaa cgctggattc actgaaaggt aaacatgttg gtgtgctgca
    35581 gggatcaacc caggaagctt acgctaacga gacctggcgt agcaaaggcg tggatgtggt
    35641 ggcctatgcc aaccaggatt tggtctattc cgatctggct gcaggacgtc tggatgctgc
    35701 gttacaagat gaagttgctg ccagcgaagg attcctcaag caacctgctg gtaaagcttt
    35761 cgcctttgct ggctcatcag taaaagacaa aaaatacttc ggtgatggca ccggtgtagg
    35821 gctacgtaaa gatgatgctg aactgacggc tgccttcaat aaggcgcttg gcgagctgcg
    35881 tcaggacggc acctacgaca agatggcgaa aaagtatttc gactttaatg tctacggtga
    35941 ctgatacgtc gctgggaagc tgtacctgat ggaatgatca tcatggtgca cgccaggttt
    36001 gttgcactat cgtggtgcat tgaaatgcat acttaagcat ttttaatgaa aaataatacg
    36061 tctaacgggg cgggatattt tgccttgatg gtcaatttta tggcacgata agtgtaacaa
    36121 acctgtaaat attccctata aaaagactgt cagttgagga cattatgaaa aaactggtgc
    36181 tatcgctctc tctggttctg gccttctcca gcgcaactgc ggcatttgct gcgattccgc
    36241 aaaacatccg catcggtacc gacccgacct atgcgccatt tgaatcaaaa aattcacaag
    36301 gcgaactggt tggcttcgat atcgatctgg caaaggaatt atgcaaacgc atcaatacgc
    36361 aatgtacgtt tgtcgaaaat ccgctggatg cgttaatccc gtccttaaaa gcgaagaaga
    36421 ttgacgccat catgtcatcg ctttccatta cggaaaaacg tcagcaagaa atagccttca
    36481 ccgacaaact gtacgctgcc gattctcgtt tggtggtggc gaaaaattct gacattcagc
    36541 cgacagtcga gtcgctgaaa ggcaaacggg taggcgtatt gcagggcacc acccaggaga
    36601 cgttcggtaa tgaacattgg gcaccaaaag gcattgaaat cgtctcgtat caggggcagg
    36661 acaacattta ttctgacctg actgccggac gtattgatgc cgcgttccag gatgaggtcg
    36721 ctgccagcga aggtttcctc aaacaacctg tcggtaaaga ttacaaattc ggtggcccgt
    36781 ctgttaaaga tgaaaaactg tttggcgtag ggaccggcat gggcctgcgt aaagaagata
    36841 acgaactgcg cgaagcactg aacaaagcct ttgccgaaat gcgcgctgac ggtacttacg
    36901 agaaattagc gaaaaagtac ttcgattttg atgtttatgg tggctaatcg ccatcggtga
    36961 aagaaagccc gctcccttcg ggtaaccgga gggagacaag aggcggtaat tcaccacaca
    37021 cgacaggaca ggcagcatgt tgtatgggtt ttcaggtgtt attttacagg gtgcgctcgt
    37081 cacgctggag ctggctatca gctctgtagt gctcgctgta atcatcggtt taattggcgc
    37141 tggcggtaag ctctcgcaaa atcggctttc ggggctgatt ttcgaagggt acaccacgct
    37201 gattcgtggc gtgccggact tagtgttgat gctgctgatt ttctacggtt tgcagattgc
    37261 gctaaacacg gtgacggagg cgatgggcgt cgggcagatt gatatcgatc cgatggtcgc
    37321 tggtattatc actctcggtt ttatctacgg tgcttatttt accgaaacgt tccgtggtgc
    37381 ttttatggca gtgccgaaag gacatataga ggcggcgacg gcgttcggtt ttactcgtgg
    37441 gcaagtgttt cggcggatca tgtttccggc gatgatgcgt tacgccctgc caggcattgg
    37501 caacaactgg caggtgatcc tcaaatctac cgcactggtt tcgttactcg gcctggaaga
    37561 tgtggtcaaa gccacccaac tggcaggcaa aagtacctgg gaaccgttct atttcgccat
    37621 cgtctgtggc gtgatttacc tggttttcac cactgtttcc aatggtgtgc tgctgttcct
    37681 tgagcgccgc tactccgtgg gtgtgaagag ggctgacctg tgatcgaaat cttacatgaa
    37741 tactggaaac cgctgctgtg gaccgacggt tatcgcttta ctggtgtggc gatcactctg
    37801 tggctgctta ttttgtcggt agtgataggc ggagtcctgg cgctgtttct ggcgattggt
    37861 cgtgtctcca gtaataaata catccagttt ccaatctggt tatttaccta tatttttcgc
    37921 ggtacgccgc tgtatgttca gttgctggtg ttctattccg gcatgtacac gcttgagatt
    37981 gttaagggaa ccgaattcct taacgctttc ttccgcagtg gcctgaactg taccgtgctg
    38041 gcgctgacgc ttaacacctg cgcttacact accgagattt ttgctggggc aatccgttcg
    38101 gttccgcatg gggaaattga agccgccaga gcctatggct tctcgacttt taaaatgtat
    38161 cgttgcatta ttttgccttc tgcgctgcgt attgcgttac cggcatacag caacgaagtg
    38221 atcctgatgc tgcactctac tgcgctggcg tttacggcca ctgtgccgga tctgctgaaa
    38281 atagcccgcg atattaacgc cgccacgtat caacctttta ccgccttcgg cattgccgcg
    38341 gtgctctatt taatcatctc ttatgtcctg atcagcctct ttcgcagagc ggaaaaacgc
    38401 tggttgcagc atgtgaaacc ttcttcaacg cactgagaat acgatgtccg agaataaatt
    38461 aaacgttatc gatttgcaca aacgctacgg cgaacatgaa gtgctgaaag gggtctcact
    38521 gcaagcgaat gccggagatg taataagcat catcggatcg tcgggatcgg ggaaaagtac
    38581 ctttctgcgc tgcattaact tcctcgaaaa accgagtgaa gggtcgatcg tggtcaatgg
    38641 ccagacgatc aatctggtgc gcgacaaaga cggtcaactc aaagtcgccg ataaaaatca
    38701 actgcgctta ctgcgcacac gcctgacgat ggtattccag cacttcaatc tctggagcca
    38761 tatgacggtg ctggaaaacg tcatggaagc gccgattcag gtgttgggcc tgagcaagca
    38821 ggaagcgcgc gagcgggcgg tgaagtatct ggcgaaagtc gggatagacg aacgtgcgca
    38881 ggggaaatat ccggtgcatc tttccggcgg tcagcaacag cgtgtttcta tcgcgcgggc
    38941 gctggcgatg gaaccggaag ttttactgtt tgatgaacct acctcggcgc tcgatcctga
    39001 actggtaggc gaagtgttac gtattatgca gcaactggca gaagagggga aaaccatggt
    39061 ggtagtgact cacgaaatgg gctttgctcg ccatgtttct actcacgtca ttttcctcca
    39121 tcaggggaaa atagaagaag agggcgcgcc ggagcagtta tttggcaacc cgcaaagccc
    39181 tcgtctgcaa cagttcctta agggatcgct gaaataattt cctctccggt ggctaacgct
    39241 gccggagtat tttcatgggg aatttgcgag tcagcttccg ctgaaatgta aaagttgcag
    39301 tccgctaaaa atacttctga attcgctgtc caaagataaa aactgcctgc tgcattgggt
    39361 gttaatcgtg gttattgtcg cgtcatctta aatttgaggg gtgacggaac gccatgacaa
    39421 tatcgacaac ttccacgccg catgatgcgg tatttaaatc ttttttacgc catccagaca
    39481 ccgcgcggga ttttattgat attcatcttc ccgcgccgct gcgcaaactg tgtgatttaa
    39541 cgacgcttaa actagaatca aacagtttta ttgatgaaga cctgcggcaa tattattccg
    39601 acctcttgtg gtctgtgaaa acgcaggagg gagtgggtta tatttatgta gtgatagagc
    39661 accaaagtaa gctggaagaa ttaatggctt ttcgcatgat gcgttattcc attgcggcaa
    39721 tgcaaaacca tcttgatgcg ggctataaag agcttccatt ggtgatcccg atgctgtttt
    39781 atcatggttg cagaagtcct tatccttatt cactctgctg gcttgatgaa tttgccgagc
    39841 ctgctatagc ccgcaaaata tattcatcag cgtttccgtt ggtggatatt accgtggtgc
    39901 cggatgacga gattatgcaa caccgcaaaa tggcgctgtt ggagttaatt cagaaacata
    39961 ttcgtcagcg cgatctgttg ggattagtcg accaaattgt ttcgctgcta gttacaggga
    40021 acactaatga cagacagcta aaagccctgt ttaattacgt attacaaaca ggggatgccc
    40081 agcgtttccg tgcatttatt ggtgagataa cagaacgcgc accacaagaa aaggagaaac
    40141 tgatgaccat tgctgacaga ttacgtgaag aaggcgcaat gcagggcaaa cacgaagaag
    40201 ccctgcgtat tgctcaggag atgctggata gaggtttaga cagagagtta gttatgatgg
    40261 tgacccgact ttcaccagac gatcttatcg cgcaaagcca ctaatcctgt aacaccggga
    40321 gttaactggc ggatgtttgc tgtaaaccac atcagcgaac gacatccgcc agcgcctctt
    40381 ctaaatcgta ccagcgaaac gcaaaacccg cttcttccag ccgtttaggc agcgcgcgtt
    40441 gtccacctaa taccagtatt gaagattcgc ccattaacag ccgaatggcg gttgcgggga
    40501 cgcgcaaaat ggccgggcga tgcagcgctt gaccgagcgc atgggcaaat tgttcattgc
    40561 gtaccgggta gggcgaaacc atattaaatg gcccgcgcag gtcgttatcc agcagccaga
    40621 gaatgccgtt gaccatatca tcaatatgaa tccaggccag atactgccga ccggaaccaa
    40681 tcggcccgcc caggccaaga cgaaacggtg gcagcatttt accgagaata ccgccgtccg
    40741 gtgccagtac cacgccggtt cgcagcaggc acactcgcgt tttgtcactt tgcgcccggc
    40801 aggcaatctc ttcccagcgg gcgcagagtt tatgagtaaa ttcgttatgc ggcggttcct
    40861 cttcggtaac caccacttca cctaagtcgc cataatagcc cgttgccgag ccggaaatga
    40921 gtaccgacgg cggcgtgtcg ctggcattaa tcaaatcgac cagtttttgc gtgatattcc
    40981 agcggctttg gcagagacgc tctttttgct cgtgagtcca gcgtttatca gcaatcggtt
    41041 ctccggccag gttgattacc gcatcaacgc cgttgaggtt gctttgatcg gctagccctt
    41101 gccatagcgt cacccgaggg ccgagaacgg aactggcttt ctgtgggtta cgcgtcacta
    41161 ccgtgatttg atggcccagc tccagcaaac gcggaatcaa atggcgacca attaatcccg
    41221 tccctccggt gatcactatg ttcatgcagc ctcccgatat ggcggttaac gctgccagct
    41281 taaggtcatg gataccgaat cggcgtagcg cagcgcgtgc agtttatcga tctccacttc
    41341 agcatacgtc acccagtgat gttcacgtgc gatatcgagc acatcctgag ttaatttttc
    41401 caacaaagag aaacggttat tctctacatg ctgaataatg tttttcgtta cggtgcgata
    41461 attcagcgca tcgttgatat cttcgctagt gcgcgcttta tcggcggggt agtggatcgt
    41521 cacattgata acaatatcct gacggttgtt aatttcttct tccttaattc cgataaacgt
    41581 acgtaaacga aggttcttta tacgaataat agcggcaggt tgtgccattg caggttctcc
    41641 gttgttttac ttaggatgaa ataatacacg agaacctgtt tctgttaact atccgaacgc
    41701 tcatcaccga gttgtgcttt tagcagtgcc tgcccggtgg cagggcgcga acggatccgc
    41761 tcatgccagt tcttgactgc cggatacatt gcgaggtcaa ttcgctggcg agtccaggca
    41821 ttaacccacg gccagcaggc aatatccgca atgctgtagt tctcgcctcc cagccagggc
    41881 gagttttcca gccgcttgtt tagtacatgg taaagacgct gagtttcaac ctgataacgt
    41941 tca
//
LOCUS       DAKISA010000029        41308 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.029, whole genome shotgun sequence.
ACCESSION   DAKISA010000029 DAKISA010000000
VERSION     DAKISA010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41308
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.029"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..877)
                     /gene="nadA"
                     /locus_tag="OVW45_001581"
     CDS             complement(<1..877)
                     /gene="nadA"
                     /locus_tag="OVW45_001581"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCV5526064.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMLTLQAECSLDLGCPVEEFNAFCDAHPDHTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSFGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCR"
     gene            complement(1126..1257)
                     /locus_tag="OVW45_001582"
     ncRNA           complement(1126..1257)
                     /ncRNA_class="other"
                     /locus_tag="OVW45_001582"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(1282..1357)
                     /locus_tag="OVW45_001583"
     tRNA            complement(1282..1357)
                     /locus_tag="OVW45_001583"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1322..1324),aa:Lys,seq:ttt)
     gene            complement(1391..1466)
                     /locus_tag="OVW45_001584"
     tRNA            complement(1391..1466)
                     /locus_tag="OVW45_001584"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1431..1433),aa:Lys,seq:ttt)
     gene            complement(1613..1688)
                     /locus_tag="OVW45_001585"
     tRNA            complement(1613..1688)
                     /locus_tag="OVW45_001585"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1653..1655),aa:Lys,seq:ttt)
     gene            complement(1692..1767)
                     /locus_tag="OVW45_001586"
     tRNA            complement(1692..1767)
                     /locus_tag="OVW45_001586"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1732..1734),aa:Val,seq:tac)
     gene            complement(1917..1992)
                     /locus_tag="OVW45_001587"
     tRNA            complement(1917..1992)
                     /locus_tag="OVW45_001587"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1957..1959),aa:Lys,seq:ttt)
     gene            complement(2157..2948)
                     /gene="cpoB"
                     /locus_tag="OVW45_001588"
     CDS             complement(2157..2948)
                     /gene="cpoB"
                     /locus_tag="OVW45_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCV5526065.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(2958..3479)
                     /gene="pal"
                     /locus_tag="OVW45_001589"
     CDS             complement(2958..3479)
                     /gene="pal"
                     /locus_tag="OVW45_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCV5526066.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(3514..4806)
                     /gene="tolB"
                     /locus_tag="OVW45_001590"
     CDS             complement(3514..4806)
                     /gene="tolB"
                     /locus_tag="OVW45_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCV5526067.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(4939..6171)
                     /gene="tolA"
                     /locus_tag="OVW45_001591"
     CDS             complement(4939..6171)
                     /gene="tolA"
                     /locus_tag="OVW45_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCV5526068.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKIKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAA
                     DKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEKAAADKKAAAAKAAAEKAAA
                     AKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAGQIK
                     SAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKP
                     PSQAVYEVFKNAPLDFKP"
     gene            complement(6236..6664)
                     /gene="tolR"
                     /locus_tag="OVW45_001592"
     CDS             complement(6236..6664)
                     /gene="tolR"
                     /locus_tag="OVW45_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCV5526069.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(6668..7360)
                     /gene="tolQ"
                     /locus_tag="OVW45_001593"
     CDS             complement(6668..7360)
                     /gene="tolQ"
                     /locus_tag="OVW45_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCV5526070.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(7357..7761)
                     /gene="ybgC"
                     /locus_tag="OVW45_001594"
     CDS             complement(7357..7761)
                     /gene="ybgC"
                     /locus_tag="OVW45_001594"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCV5526071.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(7911..8204)
                     /gene="ybgE"
                     /locus_tag="OVW45_001595"
     CDS             complement(7911..8204)
                     /gene="ybgE"
                     /locus_tag="OVW45_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCV5526072.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCLLLADIVLIVGLIFFFF"
     gene            complement(8204..8317)
                     /gene="cydX"
                     /locus_tag="OVW45_001596"
     CDS             complement(8204..8317)
                     /gene="cydX"
                     /locus_tag="OVW45_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCV5526073.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(8332..9471)
                     /gene="cydB"
                     /locus_tag="OVW45_001597"
     CDS             complement(8332..9471)
                     /gene="cydB"
                     /locus_tag="OVW45_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCV5526074.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(9487..11055)
                     /gene="cydA"
                     /locus_tag="OVW45_001598"
     CDS             complement(9487..11055)
                     /gene="cydA"
                     /locus_tag="OVW45_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCV5526075.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(11651..11878)
                     /locus_tag="OVW45_001599"
     CDS             complement(11651..11878)
                     /locus_tag="OVW45_001599"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5526076.1"
                     /translation="MRGQIVDKGRFVHAGYGMNALFGLQKQANSIYCRDDVDTGKRSA
                     SGNFAFIFTLKPRICGFIFNKIITLGELIII"
     gene            complement(11903..14536)
                     /gene="mngB"
                     /locus_tag="OVW45_001600"
     CDS             complement(11903..14536)
                     /gene="mngB"
                     /locus_tag="OVW45_001600"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HCV5526077.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLQDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVIR
                     YDMAVPLNLSERSARQSTGRVGGVLVVTLSHNSRRIDVDINLDNQADDHRLRVLVPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(14554..16470)
                     /gene="mngA"
                     /locus_tag="OVW45_001601"
     CDS             complement(14554..16470)
                     /gene="mngA"
                     /locus_tag="OVW45_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCV5526078.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            16639..17361
                     /gene="mngR"
                     /locus_tag="OVW45_001602"
     CDS             16639..17361
                     /gene="mngR"
                     /locus_tag="OVW45_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCV5526079.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEILIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(17465..18334)
                     /gene="sucD"
                     /locus_tag="OVW45_001603"
     CDS             complement(17465..18334)
                     /gene="sucD"
                     /locus_tag="OVW45_001603"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCV5526080.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(18334..19500)
                     /gene="sucC"
                     /locus_tag="OVW45_001604"
     CDS             complement(18334..19500)
                     /gene="sucC"
                     /locus_tag="OVW45_001604"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCV5526081.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(19594..20811)
                     /gene="odhB"
                     /locus_tag="OVW45_001605"
     CDS             complement(19594..20811)
                     /gene="odhB"
                     /locus_tag="OVW45_001605"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCV5526082.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(20826..23627)
                     /gene="sucA"
                     /locus_tag="OVW45_001606"
     CDS             complement(20826..23627)
                     /gene="sucA"
                     /locus_tag="OVW45_001606"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCV5526083.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKENMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRTASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(23928..24644)
                     /gene="sdhB"
                     /locus_tag="OVW45_001607"
     CDS             complement(23928..24644)
                     /gene="sdhB"
                     /locus_tag="OVW45_001607"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCV5526084.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(24660..26426)
                     /gene="sdhA"
                     /locus_tag="OVW45_001608"
     CDS             complement(24660..26426)
                     /gene="sdhA"
                     /locus_tag="OVW45_001608"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCV5526085.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(26426..26773)
                     /gene="sdhD"
                     /locus_tag="OVW45_001609"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(26426..26773)
                     /gene="sdhD"
                     /locus_tag="OVW45_001609"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCV5526086.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(26767..27171)
                     /gene="sdhC"
                     /locus_tag="OVW45_001610"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(26767..27171)
                     /gene="sdhC"
                     /locus_tag="OVW45_001610"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCV5526087.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            27552..27659
                     /locus_tag="OVW45_001611"
     CDS             27552..27659
                     /locus_tag="OVW45_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5526088.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            27865..29148
                     /gene="gltA"
                     /locus_tag="OVW45_001612"
     CDS             27865..29148
                     /gene="gltA"
                     /locus_tag="OVW45_001612"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCV5526089.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            29538..30104
                     /locus_tag="OVW45_001613"
     CDS             29538..30104
                     /locus_tag="OVW45_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCV5526090.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            30155..32613
                     /locus_tag="OVW45_001614"
                     /pseudo
     CDS             30155..32613
                     /locus_tag="OVW45_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            32628..33359
                     /locus_tag="OVW45_001615"
     CDS             32628..33359
                     /locus_tag="OVW45_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCV5526091.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            33356..34417
                     /locus_tag="OVW45_001616"
     CDS             33356..34417
                     /locus_tag="OVW45_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCV5526092.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            34569..35615
                     /locus_tag="OVW45_001617"
     CDS             34569..35615
                     /locus_tag="OVW45_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCV5526093.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLTICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(35612..36403)
                     /gene="nei"
                     /locus_tag="OVW45_001618"
     CDS             complement(35612..36403)
                     /gene="nei"
                     /locus_tag="OVW45_001618"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCV5526094.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(36439..37173)
                     /gene="pxpA"
                     /locus_tag="OVW45_001619"
     CDS             complement(36439..37173)
                     /gene="pxpA"
                     /locus_tag="OVW45_001619"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCV5526095.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQIMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQRGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(37163..38095)
                     /gene="pxpC"
                     /locus_tag="OVW45_001620"
     CDS             complement(37163..38095)
                     /gene="pxpC"
                     /locus_tag="OVW45_001620"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HCV5526096.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTDGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLVWRLHNEN"
     gene            complement(38089..38745)
                     /gene="pxpB"
                     /locus_tag="OVW45_001621"
     CDS             complement(38089..38745)
                     /gene="pxpB"
                     /locus_tag="OVW45_001621"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCV5526097.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGFSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(38768..39511)
                     /gene="ybgI"
                     /locus_tag="OVW45_001622"
     CDS             complement(38768..39511)
                     /gene="ybgI"
                     /locus_tag="OVW45_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCV5526098.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDV
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            39782..41263
                     /gene="dtpD"
                     /locus_tag="OVW45_001623"
     CDS             39782..41263
                     /gene="dtpD"
                     /locus_tag="OVW45_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCV5526099.1"
                     /translation="MNKHASQPRTIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARGSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
BASE COUNT         9992 a        11078 c        10061 g        10177 t
ORIGIN      
        1 tgcggcaggt tgcaccctca cctgcggttg gtgcttccag taactcttta tctggcaccg
       61 cctgctgcat tttgtagaaa atgccccgat cggttgccac gataagccgc tgatgtggca
      121 atgttttcgc agcagcgatc agttgactgg tggaaccgac cgcatccgcc atatcgacaa
      181 tagcttgtgg tgattctgga tgcaccagta tggcagcatc cgggtattct tcttgcaagc
      241 gggttaacgc ctgagtctta aattcatcat gcacaataca ggcaccctgc cagcataaaa
      301 tgtctgcgcc cgtctgtttt tgcacgtaac gccccagatg tttgtcgggt gcccagatga
      361 ttttttcacc aaaactatcc agatgatcaa taagttcgac ggcaatgctt gaagttacca
      421 cccaatctgc gcgcgctttt accgcagcag aagtgttggc gtagacgacg acagtatgat
      481 cgggatgggc atcgcaaaat gcgttaaatt cttcaacagg gcagccgaga tccagtgaac
      541 attcagcctg aagtgtcagc atcagaattg ttttttccgg actgagaatt ttggcggttt
      601 ctcccataaa tctcacccca gcgactaaca acgtagaagc gggatgcttt gcaccgaagc
      661 gcgccatttc cagagaatca gaaatacagc caccggtttc ttctgccagt tgttgaattt
      721 cggggtcggt atagtagtgg gcaaccatca ccgcattacg ttcttttagc agacgtttta
      781 tcttctcgcg gtaatacgct ttttcatcaa tgcttaacgg cgtcggcttc ggggggaaag
      841 gataaatcgc cgtgtctgga tcaaacatca cgctcatctt accatctcgt tttacaggct
      901 taacgttaaa accgacatta gcgtaatatt cgctactatc ggcgtaattt gttttatatg
      961 ctaaacaaga tagtcgaatg gtggggaaaa gtcactcgaa ttacgcctaa ttccaccgac
     1021 gtagagttgc ttgcacagta agctctgacg atgtcatcag gtttacaaag tgaaattgag
     1081 gatgcctgaa tagcaaaaaa gcgcctttag ggcgcttttt tacattggtg ggtcgtgcag
     1141 gatgactcgc ttcgctcgcc cttcgggccg tcgccgcgag cggctccgtt gtttcgctgc
     1201 gctcaactcg aacctgctgc aggttcgaat ctttgaatat ctcagatagc aaaaaagcgc
     1261 ctttagggcg cttttttaca ttggtgggtc gtgcaggatt cgaacctgcg accaattgat
     1321 taaaagtcaa ctgctctacc aactgagcta acgacccctt gcgggattat gctgctttaa
     1381 tcattcagga tggtgggtcg tgcaggattc gaacctgcga ccaattgatt aaaagtcaac
     1441 tgctctacca actgagctaa cgacccatac gggtgttacc tgaaagattt ctactcgggg
     1501 ccaatcttta agattggtgg gtcgtgcagg atgactcgct tcgcttgccc tacgggccgt
     1561 tgctggcgca acgttatcct tcacgcttaa catctgagtt tgatgttaaa attggtgggt
     1621 cgtgcaggat tcgaacctgc gaccaattga ttaaaagtca actgctctac caactgagct
     1681 aacgacccga gtggtgggtg atgacgggat cgaaccgccg accccctcct tgtaagggag
     1741 gtgctctccc agctgagcta atcacccgat actgcgctgg atactacaaa ctacttccac
     1801 cgaacccacc gtaattagtg gtgggtcgtg caggatgact cgcttcgctc gccctacggg
     1861 ccgttgctga cgcaacgtta tcctacacgc ttaacatctg tcttcgatgc taaaactggt
     1921 gggtcgtgca ggattcgaac ctgcgaccaa ttgattaaaa gtcaactgct ctaccaactg
     1981 agctaacgac ccactttttc gttgctttcg gtttgtttga tatcccgtgg caacggcggc
     2041 atatattact gatttcagac ttgagcgcaa caaaaatttc gatgcagatc actcaactgc
     2101 ttatgattcg cacgacacga ccagaaataa tgcgacttct ggtcgtgtgt tatgcattac
     2161 atcgcgttca gacgtttttg tgcctgttta gcgccatcag taccagggta tttactgata
     2221 acctgctggt atacggcttt cgcttttgcg gtgtcacctt tgtcctgcat gatgacgccg
     2281 actttaaaca tcgcatcagc agcctttggt gacttcggat agtttttcac taccgaagca
     2341 aaatagtacg ccgcatcatc ttttttaccc ttgttgtagt ttaactgacc cagccaataa
     2401 ttggcgtttg gcaggtaagt tgaatcaggg taatttttga tgaaattctg aaatgccacc
     2461 attgcgtcat cctggcggga tttatcctgc accagcgcaa tagctgcatt gtaatccgtg
     2521 tttgcatcgc cgcttttcac cggcgcgcca gcattcgcag taccagcatc agctgtcggc
     2581 gtcgttgatg ccgccgcacc gctttgatcg ctgctggttg attgcgccgc tgcaccaccg
     2641 ctgctgaggc tgtcgatctg caacaggatc tgcttttgcc gctccacgac ctgattcagt
     2701 tgatactgat tttcctgaat ctgaccacgc agggaatcaa tatcggattg attatcagaa
     2761 agttgttgct ggagttgggt taaaagctgg ctgtgagcat tagaaatacg ctcaagttga
     2821 gtgacgcggt cttcgaccga gcctgagccg acactactga ttggtgcctg agcaaaagcg
     2881 gcccaggggg ccgctatacc aaccagtaac gacagactca atagttgatg tctgaagtta
     2941 ctgctcatgc aattctctta gtaaaccagt accgcacgac ggtttttgga gtatgccgct
     3001 tcgtcatgac ccagtactgc aggtttttct ttaccgtaag aaacgatgga gatctggtct
     3061 gcagaaacgc ctttaccctg caggtacatc ttaacggcgt tcgcacgacg ttcacccagg
     3121 gagatgttgt attccggagt accacgttcg tccgcgtgac cttctacggt gactttgtaa
     3181 gacgggttgc tacgcaggaa gtttgcatgt gcatccagca tttgagcgaa gtcagaacgg
     3241 atatcgtact tgtccagatc gaagtaaacg atgttgttct gctgcagctg ttgcatttgc
     3301 agacgagcct gctcttcgga agacatgttg ccgttgccgc cgttcgcatc cataccagtg
     3361 ccggcaccca gcatgccttc gctgccgtca ttgctggcgt tcttgttgga agaacatgcc
     3421 gcaattgcca taacaggcag agcaatcatc agccctttca gcactttgtt cagttgcatt
     3481 tcaatgattc ctttactatt caattaatta ttatcacaga tacggcgacc aggcagggaa
     3541 tttgacctgt ccatcagttg ccggaagacg cgctttgaaa cgcccatctg tagaaaccaa
     3601 attcagcacg gatcccatcc cctgagaaga gctgtagatt accatagtgc cgttaggtgc
     3661 cagacttggc gtttcatcca ggaacgtgga cgacagaact tgtacgcctc ccgttgccag
     3721 atcttgtttg gcaatgtgct gctgcccacc gttggagctg accattacca taaatttacc
     3781 gtcgctgctg acatccgcat cctggttctg cgaaccttcc caggtaatac gttgtggcgc
     3841 accgccgttg atattcactt tataaacctg tggacgaccg gcctggtcag aagtaaatgc
     3901 caggttctgg ctgtccggga accaggtcgg ttcggtattg ttactgcgac catcagtcac
     3961 ctggcggatc tgaccagaag ccaaatccat tacgtacagg ttcaggctac cggttttcga
     4021 caaggcgaat gccagtttgc tgccgtctgg cgagaatgca ggcgcaccgt tgtgacgcgg
     4081 gaatgaagcc acctgacgta cagcgccgtt tgccagcgtt tggataacta gcgcggaacg
     4141 accgctttcg aaggtcacat aagccagttt agaaccgtct ggtgaccacg ccggggacat
     4201 cagcggctgt ggtgaacggt gaacgacaaa ctggttgtaa ccgtcatagt cagatacgcg
     4261 cagttcatac gggaactgac cgccgttggt ctgaacaacg taggcaatac gggtacggaa
     4321 cgcaccttta atgccggtca gtttttcaaa cacttcatca ctggcggtat gaccagcata
     4381 gcgcagccac tgcttgttca ctttgtacga gttctgagca agtacagtac ccggtgcgcc
     4441 gccagtgtca acaagttgat aagcaacatt gtaagaacca tccggattcg gagtgacctg
     4501 accgacaact acagcgtcaa tacccagcgc ggaccatgca gctggttgta cttcctgcgc
     4561 actacccggc tgctgtggca gacgagcgcg atctaacgga ttaaatttac cgctgttacg
     4621 caagtcggca gcaacgatgc cgccaatatc ttcaggtgcc gcaccagggc ccgcccactg
     4681 gaaaggaaca acaccaatag gacgaccgga atctacaccg ctgtcgatca caatgcggac
     4741 ttcagcatgc agaactgatg cccacagtat gagaaaacca aatgctactc gtaatgcctg
     4801 cttcatcata tctcccttat ctggaccgat ggcccacgat aatttagcag aatgttaaca
     4861 aactaaaata cacaaaacta ccagaacccc gtggcaaccg gtgcctgatg ttgaccgtcg
     4921 gaacagtcaa catcgcgatt acggtttgaa gtccaatggc gcgtttttga acacttcata
     4981 tactgcctgg cttggtggtt tcgggatctt cgcaagttta gctgctgcca acgcagcctg
     5041 acaaagtgcg ggatcgccac cttcaggttt gatatccagt aacataccat cgggtgccag
     5101 ttttatgcgc agcgtacagg ttttgcctgc ataggacgat gcgtcataga acttactttc
     5161 gatagcagat ttaatctgcc cggcatagtt attgatatcg gcccctgatg cgccattgtt
     5221 tttagtatta ccactcccgg caggcgaagc attgttccct ttcgcccctc cccccgtttt
     5281 cggtgcattc ttaccagagc ttagctcacc gaaaatatca tctgcctctg cggcagcttt
     5341 tgctgcagcg gctttttcag ctgctgcttt tgccgctgcc gcttttttgt ctgcggcggc
     5401 tttttctgcc gctgcttttg ccgctgccgc ttttttgtct gcggcggctt tttctgccgc
     5461 tgctttctta tcagctgctg ccttatcagc agccgctttt tctgctgctt ttttgtcggc
     5521 tgcagctttc tctgctgccg ctttctttct tgcttcagct gcagcagctt ctgccgcttc
     5581 cgctttcttc ttcagtgccg cagcggctgc ctcggctttt ttctgcgctt cggctgcggc
     5641 tttggcggct tctgcttctg cttttttctt cgcgtctgcc gccgctttct tcgctgcttc
     5701 ttccgcagct ttagcatccg cttcggcctt cgctttagca tctgccgccg ctttcgctgc
     5761 cgcctcttcc gcttgcttct gctttaactc ggcctgtttt gcggcttctt cagcctgctt
     5821 tttctgttcc tgagcagcta accgctcttt ctcaagttgc ttcaggcgtt cctgttcagc
     5881 cgcttgtttc tcacgcagtt cttcagcagc ctgctgttcc tttatcttgc gctgctcatc
     5941 agaacgcttc gcgcttgatt cctggctttg catgcgtttg tactgctcaa ctaccgcacc
     6001 tgaatcaacc atgacagcgt cgatggacga accaccgccg cctccggctg aagcttctat
     6061 attctcatcg aacgaactcc agatcagcgc cgcaaataag atgacatgca gcactgctga
     6121 aataattatc gcccgcttga gcttgtcgtt ttgttcggtt gcctttgaca ctctcggttt
     6181 ccaaaaactg ttcgcctgtt acccgctctc tttcaagcaa gggaaacgca gatgtttaga
     6241 taggctgcgt cattaaacca accgatttca cacccgcact atgtaacaag ttcagtgctt
     6301 taattatttc atcgtaaggc acatcttttg cgccaccgat cagaaagacc gttttcgggt
     6361 tggccttgaa acggctggac acttccgcca ccacctgctc tggtggtaaa cgctccaggc
     6421 gatctttctc aaccaccacg gtgtactgac caataccaga cacttcaaca atcactggcg
     6481 gattatcgtt actgctcacc gcctgtgatt cagtagcgtc tggcagatcg acctccacgc
     6541 tctgggtgat gatgggcgct gtcgccataa agatcagcaa cagcaccagc agtacgtcca
     6601 gcaacggtac aatgttgatt tcggacttga gatcgcgacg acctcgtcca cgcgctctgg
     6661 ccatggctta ccccttgttg ctctcgctaa cggtaaacgc ctggcggtgc agaatcgcgg
     6721 taaactcttc cataaagttg tcgtaattca gttccagttt gtttacgcgc tggttgaggc
     6781 ggttataagc cattaccgcc ggaattgcgg cgaacagacc aattgcagtc gcaatcaacg
     6841 cttctgcgat accgggcgca accatttgca gtgttgcttg ttttaccgcc ccgagggcga
     6901 taaaggcgtg catgatcccc cagaccgtac caaacagacc aatatacggg ctgatggagc
     6961 caaccgtacc gaggaacgga atgtgcgttt ccagattttc aagttcacgg ttcatggaga
     7021 tacgcatagc acgcgacgcc ccttccacta cggcttccgg cgcatggcta ttggcacgat
     7081 gcaggcgcac aaactctttg aacccgctgt aaaatatttg ttccgaacca gtcagattat
     7141 cccgtttccc ctgactctct tgatagaggc gagagagttc gattccagac cagaatttat
     7201 cttcaaacgc ttcggcttcg cgcgccgctg cgttaagaat acgggtccgc tggataataa
     7261 tggcccagga tgcgattgaa aaaccaatca aaatcaacat gataagttta accagaaggc
     7321 tagccttcag gaacaaatca aggatattca tgtcagtcac tgcttaaact ccgcgacaat
     7381 agacttggga agcgcacgag gcttcatttt gagtgggtca acgcaaacaa ccagaacctc
     7441 tgcttcattc agcaaagtat tctcggcgtt gacaatacgt tgcgtgaaaa ccaaagaggt
     7501 gccacgcatt gatgttattt cagtctgtat ttcgagcata tcgtcgagcc gcgcaggtgc
     7561 gtaatattcc accgtcattt tacgtaccac aaaggcaacg cgttcagcca tcagcgcctg
     7621 ctgactgaag tgatgatgac gcagcatctc tgtgcgtgct ctttcataaa aagcgacgta
     7681 actggcgtgg tacaccacac caccggcatc ggtatcttca tagtagacgc gaaccggcca
     7741 tcgaaacagc gttgtattca ctttacatcc cggtaatgca acaaaagtta gagcttttaa
     7801 acgctgctac tatacgcgcg cgattgaggt ttgggaatga gggcaagtta agggagcgta
     7861 aaaatttatg ggcctggagc ggcccataat tttttaaaga gaattctgat ttaaaagaag
     7921 aagaaaatca gcccgacaat gagaacaata tcggcaagca ataggcaaaa aatcccttgc
     7981 caaagaacct tctgcggacg aaagcccacg ccgtgaatca caccagcaca gacggcccac
     8041 atcagcaata aaccatgcca gatttccagt tcactggtct tcgcggcaaa acgagatggg
     8101 tcccaaaaca tacatcctgc taacagaagc gccatcacga aggaaagcgc ccgcaggggg
     8161 cgcttgtcca ttaccgcata caaggtcgcg ataatcttac tcatcagatg tcttcttgac
     8221 cggctttgcc tgattcgacg tgttcaagcg ccagcgcggt gattacccca aacgaacagg
     8281 caagaagcgt tcccagaatc catgcgaaat accacatttt tagctcctta cttagtacag
     8341 agagtgggtg ttacgttcaa tatcttcttt ggtgatacga ccgaacattt tccagtaaca
     8401 ccaagcggtg tagagcagaa tgatcggtac cagaaccacc gcaacccagg tcatgacgtt
     8461 aagcgtcagc tggctggaag ttgcatccca cattgtcaga cttgcgttca tcatggtgct
     8521 ggacggcatc acaaacggga acattgcgat accggctgtc aggatgatgc aggccagcgt
     8581 cagggaggag aacacaaacg cccacgcggc tttatccata cgtgcagtca ggatggtcag
     8641 cagcggaaga accacaccca gtgccggaat agcccacaga attggcgtgt tgttgaagtt
     8701 aaccagccat gcgccagctt cacgaaccac ttctttattc agtgggttag aggctgcgta
     8761 atggtccatt gtcgatttca cgacataacc atcgataccg tacatcaccc atacgccagc
     8821 cagtgcgaaa cagaccagtg tcaccagcgc agccacctga gccgttgcac gggtacgcag
     8881 gtgcagttcg cccacggtac gcatttgcag ataggttgcg ccctgagtaa tgatcatccc
     8941 tacgctcacc acgcctgcca gcaggccgaa cgggttaagc aactggaaga agttaccggt
     9001 gtagtacaga cgcagatatt catcaacgtt gaacggtacg ccctgcaaca ggttaccgaa
     9061 cgctacacca attaccagcg gcggaacgaa actaccaatg aagatgcccc agtcccacat
     9121 gttacgccag cgggtttctt caatcttgga gcggtagtca aaaccgaccg gacggaagaa
     9181 caaagacgcc agcacgagga tcatcgccac atagaagccg gagaacgcag cggcatagac
     9241 catcggccag gcagcaaaga gtgcgccgcc cgcggtgatc agccaaacct ggttaccgtc
     9301 ccagtgtggt gcaatggagt taatcataat tcgacgctcg gtgtcgttac gaccgaggaa
     9361 acgggtgagc atgcccaccc ccatgtcgaa accgtcagtg actgcaaaac caatcagcag
     9421 aacgccaacc agcagccacc agataaaacg caatacttca taatcgatca tttgacgact
     9481 cctgtcttag cgtgccggct gagtagtcgt ggaagactgc tcaaagtgat agcgaccggt
     9541 tttcaggctg cttgggccga ggcgtgcaaa cttgaacatt aagaacaatt ctgccaccag
     9601 gaacagggta tacaggccgc aaatcagcac cattgagaag atgagatcgc ctgcggtcag
     9661 tgacgagttc gccacagctg tcggcagcac ttcaccgata gcccacggtt ggcggccata
     9721 ttcagccacg aaccagcccg cttctacagc aatccacggc agcggaatac cgtacagcgc
     9781 ggcgcgcaga agccattttt tctcgccaat gcggttgcgg atgacactcc agaaagagag
     9841 cgcgatgatt gccagaagca ggaagccaca cgccaccatg atacggaacg caaagtacag
     9901 cggcgctaca cgcgggatgg agtctttggt tgcctgttga atctgcgctt cagtcgcatc
     9961 agccacgttt ggcgtatagc gtttcagcag cagaccgtaa ccgaggtctt tcttcatgct
    10021 attgaactgg tcacgaaccg cctggtcggt agaaccagaa cgcagttgtt cgagcagaga
    10081 gtacgccttc atcccgttac gaatgcgttc ttcatgctgc accatcagct ctttcaggcc
    10141 gataaccggg gtatccacgg aacgcgttgc aatgatgccc agtgcgtaag ggatctgaat
    10201 cgcaaatttg ttcgtctcct cttcctgatc aggaatgccg aacagagtaa aggcagcagg
    10261 cgcaggttgc gtttcccact cggcttcaat agcagccagt ttggttttct gcacgtcgcc
    10321 catttcgtag ccggattcat cacccagaac aataacagac agaacagcag ccataccgaa
    10381 gctggcagcg atagcaaagg agcgtttagc gaaggcgaag tcacgacctt tcagcatata
    10441 ccatgcgctg ataccgagga tgaacatcgc gccagtcaca taaccagacg ctacagtgtg
    10501 aacgaatttc acctgagcaa ccgggttaag caccagctcg gagaagctca ccatctccat
    10561 acgcatagtt tcaaagttga aatcggacgc gattgggttt tgcatccagc cgttcgcaac
    10621 cagaatccac agtgcggaca ggtttgaacc gagcgccacc agccaggtga cacacatatg
    10681 ctgaacttta cccagacgat cccaaccgaa gaagaacaga cctacaaagg tggattcgag
    10741 gaagaaggcc atcagacctt cgattgccag cggcgcaccg aagatatccc ctacatagtg
    10801 ggaatagtaa gaccagttag tcccgaactg gaactccatg gtcagaccgg tagccacacc
    10861 cagagcgaag ttgataccaa acaacttgcc ccagaacttg gtcatatctt tataaatctg
    10921 tttgccggag aggacgtaga ccgtttccat aatggccagc aggaacgcca taccgagcgt
    10981 cagtggcaca aaaaggaagt ggtacatcgc ggtcaaggca aactgtaagc gcgacagttc
    11041 gactatatct aacatcatga ctccttgctc atcgcatgaa gactccgaga gtgaaccccg
    11101 ttagaaaggg tcacacgcat gccccaatag aaatttgttt gaacactttc atcataccat
    11161 gtgaatgcac acaaaaacgg cgtaaagaaa ggttgcaaac atgttaataa aaactcaaat
    11221 tgatcccacg tatatattac gccgcaaaat ccttacaata aacaggtttt tgttgaagca
    11281 aatttgcatt tttcgacggt gatcaattca tagctaaatt accgcctttc agccaatttg
    11341 atcgagaaca atttatctct ttttgatgcc aatttccaag acttatacat tgataaatat
    11401 caattcctac aaattatgtt aatcatgttt caataaggta actttagtga catttatgtt
    11461 taaaatgtgt gagttataag gctgaaatta gtttcggttg gcaatttaaa gataaaaaga
    11521 gacgaagcaa aaacagcacg gttgacctta attctttttt tggtcactgg ctaatacgac
    11581 gcagagtttc aggattacta ataaaggcat aaatccaccc cgccgatatt aacatttaat
    11641 taacttgtaa ttatattata attaactcac ccagggttat tattttgtta aaaataaagc
    11701 cacatatacg tggcttgaga gtgaagataa acgcaaaatt acctgatgcg ctacgcttgc
    11761 cagtgtctac atcatctctg caatatattg aatttgcctg cttttgtaga ccgaataaag
    11821 cgttcatgcc gtatccggca tgaacaaagc gccctttgtc aacaatctgg ccgcgcatat
    11881 gtggcttcgg tttgattgct attcaggcaa gccggtaact gaacgtccgt gactggccgg
    11941 gtaaaaaagg ccccgataga tttgaacctt gattttcctc ggtggtaatg tgttcatcca
    12001 tcatcgtttc tgagcaagaa atcacctcgc gactgaaagc aacagtcgca tcacaggttg
    12061 ctgattcagc cggattaaac agccgtaaaa tcacttcttg tcggtcttca gctttcttaa
    12121 gtgcgcttat caggcatccc actgggggca ttttcaacaa actataactt tccggcacgt
    12181 tgaatccggc tttgttgagc ttcatcgcat cccatgggat tttgttgtaa cactgtactg
    12241 gagtcagcca tgctcgcgcc tgctgagcta caccagcggc ggttggcgta ccggtataac
    12301 tcaataaact taggcgacaa gaaagcagac cacgtagttg tgagtccggg actggcattt
    12361 taattcccga aggccgccca ggccttaaaa gcagatcttc tttgcccagt aagcccacgc
    12421 cacgcagcaa cgtaatggca aaggttttct tctcttcacc gatgacttca aattcacgta
    12481 acccttcgct aaagacagcc atgccgttac gcccttcctg taaggcaaca tagttgagca
    12541 tattccatac cggaaccggc gcttctttcc agccttcttg ctgccagttg ttcattgcac
    12601 tgtcgttcac ggggcgcgtt agcgaaccaa actgcgtatc tgccagaaca ctgtcagtgt
    12661 taaaaggtgt agggaccagg acacgaaggc gatgatcgtc agcctggtta tcaaggttga
    12721 tatccacatc aatacgcctg ctgttatgac taagagtgac aaccaacacc ccccctactc
    12781 tgccagtgga ttgccgggcg ctgcgttctg acaaattgag cggcactgcc atgtcatagc
    12841 ggataacagc cctgctctgc caggcttcat gaataatatc gcattgcggt ttcgcgttcg
    12901 ctgcggtaat tacccactct tcttttgcgg gtgaatagtc atattcatca ccatcatcag
    12961 agctttcttc aatttgcaat acccggtcat agcgcacacc gctgtcttta tctaccagtt
    13021 gcagagaacc atcctcattg agcgcaatct gccagaaagc attttccaga atcccttcag
    13081 cgtcactttt tgccgcaatc acgttgccag gctgattcgc ttcaatataa agcgtgcgat
    13141 agcccataga agggacaatc tggttgatct gaatatcaaa ctccataaag ggatcataat
    13201 tgccataatg aacaatttgc cgatcgatta ggccaggatc gatctcacgg gcatggcgaa
    13261 taaaatacgg tacaggctga ccgcgatcgt cttgtagatt aaactggctg gcgcgcagcc
    13321 gcacagtggt gttaataact tcttcccgcg gccagggcat caggttaaac agcacgagtt
    13381 tgtcggcatc actctgtggc atgttatcgg caattttgcg catgtagaaa cgcagcagat
    13441 tatccgccat gtcttcagcc agttcgaagc gggcgacaat ttcgcgatga actttgtcac
    13501 tgcaacaaca accgatactg tcgtgggcat gattttttaa gatctctttc cacattttct
    13561 ccagcaagcc gtggtggtat tcaaaaccca acgtccaggc cagcgttgcc agcggttcca
    13621 gcagattaac aatcttattt tcaatacgtg cgtgggcaat tttgatatcc atgcgcgtgg
    13681 aaccgatggt gcgatgtacg cgcatatatt tgccatcgat aaattcacct ttcagggtgg
    13741 ccagactttc acgctgcgct tcgatcttct caaatacctc ttcaaagcgg ctcatcacaa
    13801 acttacgttg agggtagatc tcacgtagct tatccatcac ttcgaagata ttttgctgca
    13861 atggcatctg gtcatgacca ttcggcagca aaatctcttt ggttacagac gctttttcca
    13921 gcacgtcaaa ataactgtcg aggcgtttac gtaatccgtt ttcgtcggca ggtaagtact
    13981 taccgatggc gtagcccagc ggcagcacct gcgccgtcac ttcgctaccg tcactgcttt
    14041 gccacaaaaa ctcggtttta tcagtaccgt ggcgctccga acatccgcgc cagaacatag
    14101 tgcgggtaat gccaaatcca ttgtaaatat gcggcagttg cccggacatg ccaaaggaat
    14161 ccggtaaata acctattttc atcggctcgc caaacgcgag gcagtcacgc attccgtaca
    14221 tcagattacg gacgatggat tccgcagaaa caatcgtggt atcggtctgg gtataccagg
    14281 ggccgataat caacttaccg gcttctacct gtttcttcac gcggtctttg ttttccggtt
    14341 tcaccgcgaa ataatcttcg aggatcgccg tttgcccgtc gagtacgtaa tatttgtatt
    14401 cgttgtcctg ttccagtcgg cacaggatct cttccatatt attgaccagc agaatgcgtg
    14461 actcttcggt ggtgaaatac cactcccgat cccaatgcat atgcggggtg atgtgaacgc
    14521 gagataccgc tttcatcgtc gtttcctttt tgtttatggc attacgccat cagtcagata
    14581 gttgccatgc ttaaccgcgt gacgccgcca gatcaggaga attgcagtcg agattgcagc
    14641 ccccaccaat gccgcaccaa accagccaat tgccgccata acaccgcccg cgccattatc
    14701 atgaagtaaa aagagcgaga aaatgccggc accgggtgtc gaaaggccga tattcatcgc
    14761 accgacaata gcgcccgtta ccatagagcc cagcacaaac gaaccaataa cccgcagcgg
    14821 atcttcaatc gccatcggga tcgccccttc ggtaatacct gccagcccta acagccaggt
    14881 ggatttcccg gtctcaattt caaactcttt aaacaggcgc ggtgcgagca tcgtggaagc
    14941 ggttacggta aatgccgaaa ccattttgac ggaggcgaaa atggcatacg ggccgtaaac
    15001 gccgttcgcc attgcgccca ggcagaatgc ataagcggct ttgttcactg gccctccaag
    15061 gtcaaaggaa cacataaaac cgagaatggc acccagcaac agcgcgttac ttcctgacag
    15121 accgttcagc caggcggtaa gcgagttatt gatccatgcg acaggttctc ccaccacaaa
    15181 cagcatcaga ctgcccgctc ccaacgtacc gagcaccggg tagagataaa aagtcaggaa
    15241 tccattgaat ttactgctaa gacgcaagtg atttttcacc cagcgcatca agtaaccggc
    15301 tatcaatccg ccaacgaccg cgccgagaaa cccggagccg atcatgttgg cggcaagtcc
    15361 agccgcaaag cctggcgcta acgccggttt atctgccaga gaataggcgg tataggccgc
    15421 gagcaccggt accatcaaaa ttccgagcag cccgccgccc agcttgcggt acatccacag
    15481 ccaggagttt tcttcattaa acagatcttg tagcccgaag atttgcgaca gtaataccgc
    15541 gaccgccagc accgtgcccc ccgcgacaat caacggtacg gcaaaagaga ttccgctcaa
    15601 cagtgcctgt ttcagctccg ttttgacact tttcaccggt tgcgtatctt gctgtacggt
    15661 acgcgtctca tcgctacgct tgagggtaag cgcttgttgg atcaacgctt ctgcatggcg
    15721 aatcggctcg gcaacaggca ctgaaagcgc gggaatgcca ttaaaacgct cactctcctt
    15781 gatggcgact tcagccgcaa aaatacaggc ggttgcacta ttgagttgat ccgccgttaa
    15841 acgcccttca atgccgttag cgccttgttt ttcaacgtaa acatttacgc cgagtttgcg
    15901 tccggctttt tccagatatt ccgcagccat ataggtgtga gcaatacccg ccggacaggc
    15961 cgttacgcag acgatagttg gcgcgttgga aaaagaggca gaaggttgcg tgcctccctt
    16021 gtcatccagc gccgagagca actcatcagg cgtcgtcgcc gactgtatac gcgcccgaat
    16081 ctcatcatcc gcaaggcgcg tggtcagcgc tgtcagcagt tgcatatgcg tagtacccgc
    16141 ttcattgggg ggaatcgcca gcagcaccac taaatcaact gcttccgggc catcaacgcc
    16201 ttcccactga agcggctcgc tgagtgtggc gaccgcaaac gccgcttctt ttaccgcagc
    16261 agttttgcca tgcggcacag ccaacccttc acctaaggcg gtcgggccaa ggctttcacg
    16321 gcgatacact tcttccagaa attgctcagt actggaaatt ttccccagag cagcaagacg
    16381 ttgagtcaac gcgtggatgg cctcttcacg gctggtaaag cgcgcattca gacacaacgc
    16441 atcgcggtgg gtcagagtcg tcagattcat catgatcctc acttgatcgt ttttatagtc
    16501 gcgccagtgt gcccgataaa acaataccat catgtgatca atttcacttg attgaaatta
    16561 tttgtattgt atttgtatta tttacaggat gaatttatcg ggcataatgg ctaaattcgt
    16621 attaatgaga tactaaaaat gggacacaag cccttatacc ggcagattgc cgatcgcatt
    16681 cgtgaacaaa ttgcgcgtgg cgagcttaaa cctggcgatg cgttacccac tgaatcagcg
    16741 ctacaaacag agtttggcgt cagccgggtt acggtgcgtc aggctttgcg ccagctagtc
    16801 gagcagcaga tcctcgaaag cattcagggc agcgggactt acgtcaaaga agagcgggtc
    16861 aattacgata tttttcagtt aaccagtttt gatgaaaaac tgtcggaccg tcacgtcgat
    16921 acacacagtg aaatcctgat atttgaagtg attccggctg acgattttct tcagcaacag
    16981 ctacaaatca ccgcgcagga tcgtgtgtgg catgtgaagc gcgtgcgcta tcgcaagcaa
    17041 aagccaatgg cgctggaaga aacctggatg ccgcttgcgt tgttcccgga tctcacctgg
    17101 caggtcatgg aaaattcgaa atatcacttt attgaagaag tgaagaagat ggttatcgat
    17161 cgtagcgaac aggaaatcat tcctctgatg ccaaccgaag agatgagccg tctgcttaat
    17221 atcagccaga caaagccgat tcttgaaaaa gtatcgcgtg gatatttagt tgatggtcgg
    17281 gtgtttgaat atagccgcaa cgcttttaat accgatgact ataaatttac cctgatagcg
    17341 cagcgaaaat catcgcgata agcacaaaaa aggccatcat acgatggcca accatgtcga
    17401 aaccgacagc ccttcgccat tcctgtagag ggaagcggcg agggctattt cttattacag
    17461 atatttattt cagaacagtt ttcagtgctt caccgatatc cgccaggctg cgaacggttt
    17521 tcacgcctgc ggcttccaga gcagcgaatt tctcatccgc agtccctttc ccaccagcaa
    17581 tgatcgcacc cgcgtggccc atacgtttgc ctttcggcgc agtcacacca gcgatgtaac
    17641 ctacaactgg cttggtaacg tgctctttga tgtacgctgc cgcttcttct tcagcgctac
    17701 cgccgatctc accgatcatc acgatcgctt cggtctgcgg atctttttcg aacatttcga
    17761 ggatgtcgat aaagttagag cctgggatcg ggtcaccgcc gataccaaca caggtcgact
    17821 gaccgaaacc gtaatccgtg gtctgtttaa ccgcttcata ggtcagtgta ccggaacggg
    17881 aaacgatacc cactttaccc ggtttgtgaa tgtgacccgg ctggataccg attttgcatt
    17941 cacccggagt gataacgccc gggcagttcg ggccgatcat acgaacgccg gcttcgtcca
    18001 gtttcacttt cacagtcagc atatccagcg tcgggatgcc ttcagtgatg gtgataatca
    18061 gtttgatgcc tgcatcaata gcttccagaa tggagtcttt gcagaacggt gctggtacgt
    18121 agataacaga agcggtagcg ccagtagcag caacagcttc acgcacggtg ttgaacaccg
    18181 gcaggccgag gtgggtagta ccgcctttac ctggggttac gccgccaacc attttagtgc
    18241 cgtatgcaat ggcctgttct gagtggaaag tcccctggct accagtaaag ccctggcaga
    18301 taaccttggt gtttttatca attaaaatgg acattatttc ccctccactg cggcaacaac
    18361 ctgctgagct gcatccgtca gaccttttgc tgcaataata ttcaggccgc tgtcagccag
    18421 tttcttcgcg ccgagttcgg cgttgttacc ttccagacgt accacgaccg gtacgttaac
    18481 acccacttct gctaccgcgc cgatgatacc gtcagcgatc aggtcgcaac gaacgatacc
    18541 gccgaagatg ttaaccagaa cggctttcac tttgtcgtca gagaggatga ttttgaacgc
    18601 ttcggttaca cgttctttgg ttgcgccgcc gccaacgtca aggaagttag ccggttcgcc
    18661 gccgtgcagt ttaacgatgt ccatcgtacc catcgccaga cctgcgccgt taaccataca
    18721 accgatgtta ccgtccagcg caacgtagtt cagttcccac tgtgcagcct gtgcttcacg
    18781 cggatcttcc tgcgactggt cacgcatttc gcgcagatca ggctggcgga acagtgcgtt
    18841 gccgtcagcg cccagtttgc cgtcgaggca aatcagatcg ccctgtttgg tgatgaccag
    18901 cgggttgatt tcgatcaacg ccaggtcgcg ctccaggaaa atggtcgcca ggcccatgaa
    18961 gattttggtg aactgctgaa ccagtttacc ttccagaccc agtttgaacg ccagctcgcg
    19021 tccctgatac ggcatcgggc cagtcagcgg atcaagcgca actttgtgga tcaggtgcgg
    19081 agtttcttcc gccacttttt cgatttccac gccgccttcg gtggaggcca taaagaccac
    19141 acgacgggaa ctacggtcta caacggcacc gagatacagc tctttagcga tatcggtcgc
    19201 tgcttcaacc agaatctggt taaccggttg gccattggca tctgtttgat acgttaccag
    19261 acgcttgccg agccagtttt ctgcaaaagc acggatgtct tctttgctgt ttacaacttt
    19321 cacaccgccc gctttaccgc ggccaccagc gtgaacctga catttcacta cccacgggcc
    19381 ggcaccgatt tttgaagcgg cttcttctgc ttcgcgcgga gtagtacagg cataacccac
    19441 cggtgctggt aagccatagc gggcaaaaag ttgttttgcc tgatattcat gtaagttcat
    19501 gtgttctgtc catccttcag taatcgttat cttttaaacc gtagaccgac accctatcca
    19561 cgtcggtcta cagtgcaggt gaagcttaaa ctactacacg tccagcagca gacgcgtcgg
    19621 atcttccagc aactctttga tcgttaccag gaagcccacg gattcgcgac catcgatcag
    19681 acggtgatcg taggacagcg ccaggtacat catcggcagg atctcaacct gaccattcac
    19741 cgccatcgga cgatctttga tagcgtgcat acccagaatt gcgctctgcg gcgggttgat
    19801 gatcggcgta gacatcaggg aaccgaacac accaccgttg gtgatggtga agttaccacc
    19861 ggtcagatct tcaaccgtca gcttgccgtc acggccttta actgccagct ctttgatttt
    19921 cttctcgatg tctgccatgc cgagggtatc gacatcacgc agtaccggcg tcaccaggcc
    19981 gcgcggcgtg gaaaccgcca tgctgacgtc gaaatagttg tggtaaacca cgtcatcacc
    20041 gtcgatagaa gcattcactt ccgggtaacg tttcagggct tcaaccaccg ctttcacgta
    20101 gaaggacata aagcccagac ggatgccgtg gcgtttttca aacgcttcac cgtactgctt
    20161 acgcagatcc ataatcggct tcatgttgac ttcgttgaac gtggtcagca tggcggtgga
    20221 gtttttcgct tccagcagac gctctgccac acgcttacgc aggcgagtca tcgggacacg
    20281 tttttcacta cgtgcagcca gagccggttg cgccgccgga gcagccgctg ccggagcaga
    20341 ctctttcgcc ggggctttcg ccagatgttt ttccacatct tcacgagtca gacgaccacc
    20401 cacaccggtg cctttaatgg cgctggcgtc gagattgtgt tcagccagca ggcgacggat
    20461 cgccgggctt aacgcatcgt tgttttgctc ttccagagac gcctgctggc gttgcgccgg
    20521 agtggacgct ttctcttcag atttggcgct ggtttcttta ccggcgctgt tgccttcacg
    20581 caggcgacca aggatctgac gagacgttac cgttgtacct tcatcttcca gaaccgcatc
    20641 cagaatgccg tctgctgatg ccggtacttc cagtaccact ttgtcagttt cgatttctac
    20701 cagcacttca tcacgtacga ctgcgtcgcc gggtttttta tgccaggttg cgacggtggc
    20761 atcggctacg gattcaggca ggtcagggac cagaatatct acgctactca ttgtgtatcc
    20821 tttatttatt cgacgttcag cgcgtcatta accagatctt gttgctgttt ctggtgaacg
    20881 gacatatacc ctaccgccgg agaggcggag gccgtgcggc ctgcataacg cagagaagcc
    20941 ccaaacggaa tcacttcacg gaaatgatgc tggctgcagt accatgcgcc ctggttgagc
    21001 ggctcttcct ggcaccagac aaaatccttg acgtgagcaa actgctgcaa cacttcctgc
    21061 atcgctttat gcgggaacgg gtagagttgc tcgatacgca caatggcgac atcgtgttga
    21121 ttgttcttac gacgctgttc cagcaggtcg taataaacct taccagaaca catcactacg
    21181 cgcttcacgc ccttcggatc aagctcgtcg atttcaccga tggctggcag gaaggtgccg
    21241 ttcgccagtt cttcgaggct ggaaaccgcc agcggatgac gcagcaggga tttcggcgac
    21301 atcactacca gcggacgacg catcccgcgc agcgcctgac gacgcagcat gtggtaaacc
    21361 tgtgccgggg tagacggaac gcaaacctgc atgttttgct cagcacaaag ttgcagataa
    21421 cgttccagac gcgcggagga gtgctccggc ccctgccctt cgtaaccgtg cggcagcaac
    21481 atcaccagac cacacatccg gccccatttc tgttcgccag aagagatgaa ctggtcgata
    21541 accacctgcg caccgttggc gaagtcaccg aactgcgctt cccagatggt cagagtgcgt
    21601 ggttctgcgg tggcataacc gtattcaaac gccagcactg cttcttcaga cagtacggag
    21661 tcccagacgc ggaacgcgcc ctgcccgtta tggatgtgtt gcaacggcgt gtaagtggaa
    21721 ccgttagact ggttgtggat caccgcgtgg cggtggaaga aggtaccgcg accggagtct
    21781 tcacccgaca ggcgaaccgg aatgccttcg tcaaccagcg tggcgtaagc gaggttttcc
    21841 gcaccgcccc agtcgaacag tttctcaccg gcagccatcg cctggcgatc gccataaatc
    21901 ttggcaacgc gagactgcat ttcaactgct tccggcaccg tgctgatgcg tttcgccagc
    21961 tcctgcaggc gcttcatctc aactttgttc gggtactctt cgtcccattc gtggttgagg
    22021 tacggcgacc aggtgaaaga gtgcatgttc atcggacgcc actctgccac tacgcaatcg
    22081 ccagcatcca gcgcatcgcg gtacaggtta accatctcgg tggcatcttc cagcgtcgcc
    22141 actttttcct gctccagctt gtcagcgtag attttgcgcg gtgtcggatg ttttttgatt
    22201 ttctgataca tcagcggctg ggttgcgctc ggctcgtcgg cttcgttgtg gccgtgacgg
    22261 cggtagcaca ccaggtcgat gaagacatca cgtttaaagg tgttacggaa atcgagcgcc
    22321 agacgggtca caaaggcaac ggcttccgga tcatccgcgt taacgtggaa aatcggtgcc
    22381 tgaaccatct taccgatatc agtacagtac ggtgtagaac gagcatccag tggattagag
    22441 gtggtgaaac caacctggtt gttgataaca atacgtaccg taccgccaac ttcataacca
    22501 cgcgctttcg acatgttcag agtttcctga accacgccct gcccggtcac tgcggcgtca
    22561 ccgtggatgg tgattggcag cactttgttg ctgctcggct catcaagtct gtccagacgg
    22621 gcacgaacag aaccgataac taccgggctt acaatctcaa ggtgagacgg gttaaacgcc
    22681 agcgccaggt gcaccaggcc gccatcggtc tggaagtcag acgagaagcc catgtggtat
    22741 ttcacgtcac ccgtgccgag gtgttcttta tgtttaccgg caaactcgtc gaacaagtct
    22801 tgcggttttt tacccagcac gttcaccagc acgttcagac gaccacggtg cgccatccca
    22861 agaaccactt cgcgggtgcc gctgttgcca gcgtggcgga tcatctcttt aagcatcggg
    22921 attaacgcgt caccgccttc cagcgagaag cgttttgcgc cagggaattt tgcgccgagg
    22981 taacgttcaa ggccttcagc ggcggtcagt tcgcttaaga agcgtttttt ctcttcgcta
    23041 ttgaaagtcg cgcgaccaga ctcgatacgc tgttggatcc agcgtttttc ttcggtgctg
    23101 gtaatgtgca tatactcggc accaatcggg ccacagtagg tttgcttgag ggcttccagc
    23161 agctcgccga gtttcatgtt ttctttgccg ctggcaaatg aaccgacgtt gaaggtctcc
    23221 tggaagtctg cttcggtcag atcgtggaaa gacggatcca gatcggccac tttatcttgc
    23281 tgccacagtc ccagcggatc gagattcgca tgctggtgac cacggaagcg gtatgcgtta
    23341 atgagctgca ggactttaac ctgcttcaca ttggtgtcag ggtcggagat cgttgaagag
    23401 taacgtgaag cgtctttcgc caggcggcgg aaatattcac gcgtttgaga gtggaattga
    23461 tccggtttga ctcccgtacc aggtaactgc tggaacgtcg aacgccagtt agcgtcaacc
    23521 gagtcaggat cggttaagaa gtcttcatag agctgttcta tccagctctg gtttgcgcca
    23581 gagaggtaag aagagtccaa ccaggctttc aaagcgctgt tctgcatcgt gatcccttaa
    23641 gcatcttttt tatgcttact tcgccgtgga tactaccacg cacagtgatg tgcgtgccgg
    23701 ggttcacttg cgagctcttg tctatgtagt ggctcgcgaa ggaaccttta aaaactgtct
    23761 tagtgtcagg ggcagttttt aaagatttcc tgcatttctt tgttacgcct gatgcgctgc
    23821 gcttatcagg cctacgggta atgacttgta ggccggataa ggcgttcacg ccgcatccgg
    23881 cactggttgc ctgatgcgac gcttgcgcgt cttatcaggc ctacggttta cgcattacgt
    23941 tgcaacaaca tcgacttaat atggccgatg gcgcgcgtcg ggttcagccc cttcggacat
    24001 acactgacgc agttcatgat gctgtgacag cggaatacgc tgaatgcatc gctcaaaccg
    24061 tcgaggcggc tgtcagtctc ggtatcacgg ctatcgatca ggaaacgata tgccgctaac
    24121 aagcctgccg ggccgataaa cttatcggga ttccaccaga aagacggaca agaggttgaa
    24181 caacatgcgc agagaataca ttcatacagc ccgtcgagtt tttcgcgctg ctctggcatc
    24241 tgtaaatgct cgcgagctgg cggattttgt ccattattca acaggtaagg cttaattttc
    24301 tcatattgcg catagaattg tcccatgtct accaccaaat cgcggatcac cggtaaacct
    24361 ggcagcgggc gaatcacaat cttcttgccc ggctggttga gtgccgaaat cggggtaata
    24421 caggccagac cattcttgcc gttcatgttc agaccgtcgg aaccgcacac accttcacgg
    24481 caggagcggc ggaacgacag gctgggatct ttctctttca gctggataag cgcatccagc
    24541 agcatcatgt cgcgaccttc ttccgcttcc agggtgtaat cctgcatacg cggagcatca
    24601 tcaacatccg ggttatagcg ataaattgaa aactcgagtc tcattttcct gtctccgcat
    24661 tagtaagtac gaatcttcgg cgggaatgcc gggcgcagtt tcggttccat gttgacgctt
    24721 cggcgcgtca tggattccga ctctggcaga tacagggagt ggcacagcca gttttcatca
    24781 tcacgatccg ggaagtcgaa gcggctatgt gcgccacggc tttcggtacg gaagttggca
    24841 gaaacagccg ttgcatacgc cgtttccatc aggttatcca gttccaggca ctcaacgcgc
    24901 tgggtgttga actcgctgga agtgtcatcc agacgggcat ttttcagacg ctcgcggatc
    24961 actttcaact gctcaagccc tttcgccatc gcatcacctt cacggaagac cgagaagtta
    25021 tgctgcatac attcttgcag cgctttacga atcgccaccg gatcttcacc gttacgatta
    25081 ttgttccagc ggttcagacg atccagagat gcttcaacat cagactcgct ggcatcgcgc
    25141 aatgcgccct gttcggcgat agactcttgc agatgcagac ctgccgcacg accaaagacc
    25201 accaggtcca gcagcgagtt gccgcccaga cggttagcgc cgtgtaccga tacacaagcg
    25261 atttcaccaa cggcaaacag ccccggaaca accacatctt cgcctttctc gttcaccgtc
    25321 agcgcctgac cggtcacttt ggtcggaata ccgcccatca tgtagtgaca ggttgggata
    25381 accggaatcg gctctttcac cggatcgacg tgagcaaagg tacgggaaag ctccaggata
    25441 cccggcagac gggattcgag aacttcttta cccaggtgat cgagtttcag tttcgcgtgt
    25501 ggcccccacg gaccatcaca gccgcgacct tcacggattt caatcatgat ggaacgcgcc
    25561 accacatcac ggcccgccag gtctttggcg ttcggcgcat aacgctccat aaagcgttcg
    25621 ccatgtttgt tcagcagata accgccttca ccacggcaac cttcggtgac cagtacgccc
    25681 gcaccggcaa tgccggtcgg gtggaattgc cacatttcca tatcctgcac cggtacgcct
    25741 gcgcggatag ccatgccgac accgtcgcca gtgttaatgt gggcgttagt ggtggactga
    25801 taaatacgcc ctgccccgcc agtcgccagc acggtagcgc gggctttgaa ataaaccact
    25861 tcaccggttt cgatgcacag tgcggtacaa cccaccaccg cgccatcctg gtttttcacc
    25921 agatccagcg cataccactc ggagaaaatg gtggtgtggt ttttcaggtt ctgctgataa
    25981 agcgtgtgca acagtgcgtg accggtacgg tcagccgccg ccgcagtgcg tgccgcctgc
    26041 tcgccgccga agtttttcga ctgaccgcca aacggacgtt gatagatacg accatcatca
    26101 agacgcgaga acggcaggcc catgtgttcg agttccagaa tcgcttccgg cccggtttta
    26161 cacatatatt caatcgcgtc ctggtcaccg atatagtccg acccttttac ggtgtcgtac
    26221 atatgccatt cccagttatc ttcatgggta ttacccagcg caacggtaat gccgccttgc
    26281 gcagaaacgg tatgggaacg ggtcgggaag actttagaga gcagcgcaca ggtctggccg
    26341 ctctgggaaa tttgcagcgc cgcgcgcata cctgcgccac cggcaccaat cacaactgca
    26401 tcaaattctc tgactggcaa tttcatcaca caccccacac cacaacgaat ccataaatca
    26461 cgtaaaccac cagtgcaacg acaatcacca gttgcagcat caggcgcaat gccagcggtt
    26521 taacgtagtc ggtcaacacc tgccacatgc cgatccaggc atgaatcaag atagaaaaca
    26581 gcgccagcag ggtgaacact ttggtgaacg cagaggcgaa gaaaccgatc cagacttcat
    26641 atgtcagttc gccactggtg gcgaaaaaac cgaccatata aatgatgtag agcgtcagga
    26701 cgatagcggt agcgcgaacg aggatgaaat catgtacgcc attgcgtcct aatgcggagg
    26761 cgttgcttac catacgagga ctcctgcgag aagtgaaagc acgacagtaa taacaaagga
    26821 gattttggcg gagcgtttac ccgcttcgaa tgtttcttcc agatagccaa aatccatcat
    26881 catgtggcga atacctacga cgacgtgata cgccagagcg gtaaggatgc cccacatgat
    26941 aaatttgacg aagaagctgc ccataatcgc ggaagcttgc tcgaaacctt caggggaaga
    27001 gaggctggta cccagaagcc acagcaggat gcccactgca acaaaggtga tcacaccgga
    27061 aacgcgatgg agaatggacg ctatcgccgt gatggggaac cggatggtct gtaggtccag
    27121 attaacaggt ctttgttttt tcacatttct tatcatgaat aacgcccaca tgctgttctt
    27181 attattccct ggggactacg ggcacagagg ttaactttct gttacctgga gacgtcggga
    27241 tttccttcct ccggtctgct tgcgggtcag acagcgtcct ttctataact gcgcgtcatg
    27301 caaaacactg cttccagatg cgaaaacgac acgttacaac gctgggtggc tcgggattgc
    27361 agggtgttcc ggagacctgg cggcagtata ggctgttcac aaaatcatta caattaacct
    27421 acatatagtt tgtcgggttt tatcctgaac agtgatccag gtcacgataa caacatttat
    27481 ttaattttta atcatctaat ttgacaatca ttcaacaaag ttgttacaaa cattgccagg
    27541 aaaagcatat aatgcgtaaa agttatgaag tcggtatttc acctaagatt aacttatgta
    27601 acagtgtgga agtattgacc aattcattcg ggacagttat tagtggtaga caagtttaat
    27661 aattcggatt gctaagtact tgattcgcca tttattcgtc atcaatggat cctttacctg
    27721 caagcgccca gagctctgta cccaggtttt cccctctttc acagagcggc gagccaaata
    27781 acaaacgggt aaagccaggt tgatgtgcga aggcaaattt aagttccggc agtcttacgc
    27841 aataaggcgc taaggagacc ttaaatggct gatacaaaag caaaactcac cctcaacggg
    27901 gacacagctg ttgaactgga tgtgctgaaa ggcacgctgg gtcaagatgt tattgatatc
    27961 cgtactctcg gttcaaaagg tgtgttcacc tttgacccag gcttcacttc aaccgcatcc
    28021 tgcgaatcta aaattacttt tattgatggt gatgaaggta ttttgctgca ccgcggtttc
    28081 ccgatcgatc agctggcgac cgattctaac tacctggaag tttgttacat cctgctgaat
    28141 ggtgaaaaac cgactcagga acagtatgac gaatttaaaa ctacggtgac ccgtcatacc
    28201 atgatccacg agcagattac ccgtctgttc catgctttcc gtcgcgactc gcatccaatg
    28261 gcagtcatgt gtggtattac cggcgcgctg gcggcgttct atcacgactc gctggatgtt
    28321 aacaatcctc gtcaccgtga aattgccgcg ttccgcctgc tgtcgaaaat gccgaccatg
    28381 gccgcgatgt gttacaagta ttccattggt cagccatttg tttatccgcg caacgatctc
    28441 tcctatgccg gtaacttcct gaatatgatg ttctccacgc cgtgcgaacc gtatgaagtt
    28501 aatccgattc tggaacgtgc tatggaccgt attctgatcc tgcacgctga ccatgaacag
    28561 aacgcctcta cctccaccgt gcgtaccgct ggctcttcgg gtgcgaaccc gtttgcctgt
    28621 atcgcagcag gtattgcttc actgtgggga cctgcgcacg gtggtgctaa cgaagcggcg
    28681 ctgaaaatgc tggaagaaat tagctccgtt aaacacattc cggaatttgt tcgtcgtgcg
    28741 aaagataaaa atgattcttt ccgcctgatg ggcttcggtc accgcgtgta caaaaattac
    28801 gacccgcgcg ccaccgtaat gcgtgaaacc tgccatgaag ttctgaaaga gctgggcacc
    28861 aaagatgacc tgctggaagt ggctatggag ctggaaaaca tcgcgctgaa cgacccgtac
    28921 tttatcgaga agaaactgta cccgaacgtc gatttctact ctggtatcat cctgaaagcg
    28981 atgggtattc cgtcttccat gttcaccgtc attttcgcaa tggcacgtac cgttggctgg
    29041 atcgcccact ggagcgaaat gcacagtgac ggtatgaaga ttgcccgtcc gcgtcagctg
    29101 tatacaggat atgaaaaacg cgactttaaa agcgatatca agcgttaatg gttgattgct
    29161 aagttgtaaa tattttaacc cgccgttcaa atggcgggtt aatttttata tgcccaaaca
    29221 caaaaaattg taaaaataaa atccactatc ggacattcat atattctaaa aaaaatatat
    29281 cggtataaat tataaacaaa tcgatgattt ttaatcatct atcttcctct cagttacatg
    29341 aataaaccac caaatatagc gcacccctat aatttccaat actttattca tcaaacattt
    29401 aattcatacc atcaattaat tacttttaca tttaataaaa ttaataatat ttttaagaaa
    29461 aactaaaaac attcgacaat caaataagat attcttgcac cgatatactt ttttcttttc
    29521 tgttaaagga ctcagttatg tttaaaggac aaaaaacatt ggccgcactg gccgtatctc
    29581 tgctgttcac agcacctgtt tatgcggctg atgaaggatc cggtgaaatt cactttaaag
    29641 gtgaagttat tgaagcaccg tgtgaaatac atcaggatga tattgataaa gaggttgaac
    29701 tcggtcaggt gaccaccagc cacattaatc agtcacatca cagcgatgcc gttgctgtcg
    29761 acctgcgctt agtcaactgt gatctggaaa actccagcaa cggttccggt ggcaagattt
    29821 ctaaagttgc agtcaccttt gatagctcag cgaaaaccac cggcgcagat ccgattctca
    29881 acaacaccag cacgggtgaa gccaccggcg tcggcgtacg tttaatgaat aaagatcaaa
    29941 gcaatatcgt tttaggcacc gctactccag atatcgacct ggctccgacc tccagcgaac
    30001 aaacgctgaa tttctttgcc tggatggaac aaattgatca ggctacacct gtaacgccag
    30061 gcgcagttac ggcgaatgca acctacgtgc tggattataa ataagtatta ctttggcggc
    30121 gctatcgccg cctttcataa aatatttatc aggtatggac accgtgaata tttatcgact
    30181 ctcttttgta tcctgcctgg tcgtggcgat gccttgcgca ttggcggtcg aattcaacct
    30241 taatgttctc gataaatcga tgcgcgaccg cattgatatt tcattattaa aggaaaaagg
    30301 agtcattgct cccggtgagt attttgttag cgttgcggta aataataacc aaatcagtaa
    30361 cgggcaaaag attaactggc acaaaaatga cgataaaacc attccgtgca tcaatgattt
    30421 actggtcgat aaatttggct taaaacctga agtccgtcag tcgttaccat tgataaatca
    30481 gtgcgtcgat tttagctccc gacctgaaat gctcttcaat ttcgatcaag ccaatcagca
    30541 actgaatatt agtattccgc aagcctggct ggcgtggcac tcagaaaact ggaccccacc
    30601 ctccacatgg aaagaaggtg tcgccggtat cctgatggat tacaacttgt ttgccagcag
    30661 ctaccgccca caggacggca gcagcagcac taacctgaac gcctacggta ccaccggaat
    30721 taacgccggg gcatggcgct tacgtagtga ttatcagttg aatcagactg atagcgatga
    30781 taaccatgaa cagtcaggcg gaatatcgcg cacctatctt tttcgtccat taccgcaatt
    30841 aggctctaaa ttaaccctcg gcgaaacgga ttttagttcc aatattttcg acggtttttc
    30901 ttataccggc gcggcactgg caagtgacga gcgaatgttg ccatgggaac tacgcggcta
    30961 cgccccacaa attagcggta ttgcacagac caatgccacg gtgacgatca gtcaatcagg
    31021 ccgcgtcatt taccagaaaa aagtcccacc aggcccattt atcattgacg accttaatca
    31081 gtctgttcag ggcacactgg atgtcaaagt gacggaagaa gatggtcggg tgaacaattt
    31141 ccaggtttcg gcagcatcga cgcccttcct gactcgtcag ggacaggttc gctataaact
    31201 ggccgcgggt cagccacggc cctccatgtc acatcaaact gaaaatgaaa ccttttttag
    31261 caatgaagtt tcctggggga tgctgtcaaa cacctcgctg tacggcggcc tgctgctttc
    31321 tggtgatgac taccattctg ccgcaatggg tattgggcaa aatatgctgt ggcttggtgc
    31381 gctgtcgttt gatgtcacgt gggccagtag ccattttgat actcagcagg acgagcgggg
    31441 cttaagctac cgttttaatt acagcaaaca agtggatgcc actaacagca cgatttccct
    31501 cgccgcttat cgtttctccg atcgtcattt tcacagctac gccaactatc tggatcacaa
    31561 atacaacgac agcgatgcgc aggacgaaaa acagacgatc agcttattgt gggccaaccg
    31621 attaccccac taaacctcaa tctttacgcc aacctgctac atcaaacctg gtggaatgca
    31681 gacgcctcca cgaccgccaa catcacggca ggttttaatg ttgatattgg tgactggaga
    31741 gatatctcta tttcgacgtc attcaataca acccattacg aagataaaga tcacgacaac
    31801 cagatttacc tgtcgatttc gctccccttc ggtaacggtg gtcgggttgg ctatgacatg
    31861 caaaacagta gccacagcac cacacaccgc atgtcgtgga acgatacgct ggatgaacgt
    31921 aatagctggg gcatgtctgc cggactgcaa tccgaccgtc ctgacaatgg agcccaggtg
    31981 agcggtaact atcagcacct gagttcagcg ggtgagtggg atatttctgg tacctatgcc
    32041 gccaatgatt acagttccgt cagcagcagc tggagcggtt ctttcaccgc aacccaatat
    32101 ggtgcagcgt ttcatcgccg cagctccacc aatgaaccac gcctgatggt cagcaccgat
    32161 ggcgtggcag atattccggt tcagggcaat ctcgactaca ccaaccattt tggcattgcg
    32221 gtggtgccgt tgatttccag ttaccagcct tccaccgtgg cggtgaacat gaatgactta
    32281 cccgacggcg taacagttgc agaaaacgtt atcaaagaaa cgtggattga aggcgcgata
    32341 ggttacaaat cactggcttc ccgttccggt aaagacgtta acgtcatcat tcgcaacgcc
    32401 agcggtcagt ttcctcccct cggagcggat atccgccagg atgacagcgg cattagcgtg
    32461 gggatggttg gcgaggaagg acatgcctgg ttaagcggag tcgctgaaaa tcaaaagttt
    32521 accgtggtct ggggtgatag ccagcattgc tcgctccatc ttcctgaaca tatggaagac
    32581 accgcaaatc gcctgatttt accttgtcat taattaagga aatgaaaatg acatttatga
    32641 aaggactacc ccttttgttg ttggttgcca gtctgtgcag ccacgctgca ctacaacccg
    32701 atcgcactcg tattgtgttt aacgctaatg ataaagctac cagtctgcgg gtggataatc
    32761 gtagcgataa actcccctac ctcgcttatt cctggctcga aaatgaaaaa ggggagaaaa
    32821 gcgatgatct tctggtcgcc ctgccgccga tacaacgtct ggaaccgaaa gcaacaacgc
    32881 aggtacggat agtaaagcaa gcctcgacca caaaattacc gggcgatcgc gaaacgctat
    32941 ttttctacaa tatgcgtgaa attcctcccg ccccggaaaa aaacagcgac catgccgttc
    33001 tgcaagtcgc cattcagagt cgtatcaagg tgttctggcg accggctgca ttacgcaaga
    33061 aagcaggaga aaaggtcgaa ctgcagttac aggtcagcca gcagggcaac caactgacac
    33121 tgaaaaatcc taccgcgtat tatctgacca ttgcctacct gggacgaaat gaaaaaggcg
    33181 ttctccccgg cttcaaaacc gtgatggttg cgccttttag tacggtcaac acaaacaccg
    33241 gaaattatag cggcagccag ttttatctcg gttatatgga tgattacggc gcgttacgca
    33301 tgacgacgct caactgcagc ggacaatgcc atttacaagc cgtggaggcg aagaaatgag
    33361 tgctggcaag ggagtgttgc tcgtcatctg tctgttattt ctgccgttga agtctgcgct
    33421 tgccctgaac tgctactttg gttcatccgg tggctcggta gaaaaatcag aagcgattca
    33481 accttttgct gtaccaggca atgccaaact cggcgataag atttgggaat ctgacgatat
    33541 taaaattccc gtctattgtg acaacaatac caacggcaat tttgaaagtg agcatgttta
    33601 tgcctgggta aacccctacc cgggggtgca ggatcgctat tatcaactcg gcgtaactta
    33661 caatggcgtt gattatgacg ccaaccaggg aaaaagccgc atcgacacta accagtgtat
    33721 tgacagtaag aacattgaca tttatacccc tgagcagatt atcgcgatgg gatggcagaa
    33781 caaaatttgc tcgggtgatc ccgctaatat tcacatgtcg cgtacctttc tcgctcgcat
    33841 gcggttatac gtcaaaatac gagaaatgcc gccgcatgat tatcaaagta cgcttagcga
    33901 ctatatcgtc gtgcaatttg acggtgccgg tagcgttaac gaagacccta ctgcccaaaa
    33961 cctgaaatat catattactg gtctggaaaa cattcgcgtg ctggattgca gcgtcaattt
    34021 ttccatttca ccggaaacac aggtgattga ttttggtaaa tttaatttgc tggatattcg
    34081 tcgccacaca atgtcgaaaa cattcagtat taagacgaca aaatcacaaa atgaccaatg
    34141 caccgacgga tttaaagtca gttcctcctt ttataccgaa gaaacgttgg ttgaagaaga
    34201 taaagcattg ttgattggta atggtctgaa gctacgtttg ctggatgaaa acgcctcgcc
    34261 ttataccttc aataaatatg ctgaatacgc cgatttcacc agcgacatgt tggtctacga
    34321 aaaaacctat acagctgaac tttcgtccat tgcaggtacc cccatcgagg ctgggccctt
    34381 cgatacagtg gtgcttttta aaattaacta taactgattt gccatgatgc cctttgcagc
    34441 cggaaagggc atctctttca gaaaattgct gattttcccc ttttccttga ttgctgttat
    34501 tttttcctgg cccggctttc tttatgctgt atcgatacac caaagagaaa ataatgaggg
    34561 agcgaaggat gccagttttg cagtggggaa tgttatgtgt gctatcactt ctcctttcta
    34621 ttggtttcct cgcggtacat ctcccggcgg cgctattact tgggccgatg atcgccggga
    34681 tcatcttcag tatgcgcgga attactctgc aacttccccg ctccgctttt ctcgccgcgc
    34741 aggccattct tggctgcatg attgcgcaaa acctcactgg ttcgattctc accacgctgg
    34801 ccgccaactg gccgatcgtg cttgctattt tactggtgac gctgctttcc agcgccatcg
    34861 tgggttggtt attggtgcgc tatagctcac tgcctggaaa taccggtgcc tggggttctt
    34921 ctccaggcgg cgcagcggca atggtcgcta tggcgcaaga ttacggcgca gatattcgcc
    34981 tggttgcatt tatgcaatat ctgcgagtgc tgttcgtcgc gggggccgct gttctggtca
    35041 cccgcatgat gctgggtgat aacgctgaag cggttaacca gcaaatcgtc tggttcccgc
    35101 cagtgagcat taatctcctg cttacgattt tgctcgcagt cgttgccggt acggcgggat
    35161 gcatgctgcg tcttccttcc ggcacgatgc tcatcccgat gctggcgggc gcagtgctcc
    35221 agtctggtca gctcatcacc atcgaactac cggaatggct gctggcgatg gcgtatatgg
    35281 caattggctg gcggattggt cttggtttcg ataaacaaat cttactgcgg gcactacgcc
    35341 cgctaccgca aatcctgctg tcgatttttg ctctgctgac tatttgtgcg ggtatggcgt
    35401 gggggctgac ccggtttatg cacattgatt ttatgactgc ctacctcgcc accagtcccg
    35461 gcgggcttga taccgtggcg gtcatcgccg cagggagcaa tgccgatatg gcgcttatta
    35521 tggcgatgca aaccctgcgc ctgttcagta ttttgctgac ggggccagcc attgcgcggt
    35581 ttatttcaac ctatgcgccg aagcggtcgg cctagtgctg gcagccaggg caccagtaaa
    35641 acgggcgaga tgacagcgtg gttttctcaa tgatgctgcc acagcgttcg cacggttcgc
    35701 catcacgatg aaaaacctta aagcgaaaca gcgccccatg atgcttattc tcatccacct
    35761 gccccctcgt agcgtaggaa aatcgaggaa tatccagtaa cgcgtgtgcg agtgcatcca
    35821 gttgcgccgc attgagatct ttcgctttat gatttccagt caacccaacc tgccagagga
    35881 tctccacccg caaataattg ccaagcccag ccagaaacgc ctgatcgagc agtaatcctg
    35941 caaactgacg gttacgaaag cgcggtgaca ataaccgttc ctttaccacc tccggcgtca
    36001 gattcggatc cagcacatcg ggaccaacgc gttgtaaaaa cggatgcgtg gtcagttgtt
    36061 ccggcctcaa catctcaata tccgaggcgc tataaagcag aatggttttg tcagccgttt
    36121 gcagttttac ccgcaatact cgcgtggtct gcggctcttc gccggtatca accacgcgcc
    36181 agacgccgta aagctgatta tggctgtaga gcgttaagtc gttggaaaaa tgagttaaca
    36241 acgccttacc acgcgtttcc acatgggtaa cgtgttgacc gataagttgt gattgataag
    36301 tttttaactg cgggaaggca aaccagacat cagttagtgg tttgcctttg atcgccgcct
    36361 ccaggttatc cgctgcacgg cggatctccg ggccttcagg catggtgata tccttttttc
    36421 attctttatt aatcggggtt atgctgcgac aacaatctcc ttttcggcaa atgtagagcg
    36481 tagtcggcgg gcgaacgcca gtgcgtgctc gccgtcgcca tgcaggcaga ctgtttgcgc
    36541 cgtgaccgtt gcccattcgc cggtgatact tttgactctg ccgtgttgca ccatctctag
    36601 cgtttgcgcc aacgcctgtt cttcgttttc aatcaacgcg cctcgctgac ttcgcggcac
    36661 cagcgagccg tcagcctgat aaccgcgatc ggcaaacact tcctcgcgcg ttgtcaggcc
    36721 atattgcttg cctgcacgaa tcagctcgct tcctgccagc ccaacgagaa tcaatgccgg
    36781 atcgcaagcg tataccgctc tggcgatggc gtctgccagt tgtgcttctt tcgccgcctg
    36841 gttgtacaac atgccgtgcg gtttgacatg acgcattacg ccgccttgcg cacgggcaat
    36901 cgttgccagc gcgccaattt gatacagcgt ctgggcgtaa acggtttctg gcggcagctg
    36961 catggcgctg cgaccaaaat tttccctgtc gggaaaactc gggtgagcgc caatcgcgac
    37021 accatttttt attgcttcac gcacgcaagc ctgcatgatt tgcgcatcgc ctgcatgaaa
    37081 tccacaggca atattggcag aggaaaccag cgttaatagc tctgcgtcgc tggcgcagcc
    37141 ttcgcccaga tcggcgttca ggtcaatttt cattgtgcag ccgccacact aattgttcga
    37201 aataacgttg ctgatcttgc cgcgctttca gtgcctcttc cagtgaacac tggacaaaat
    37261 gaatcggctg accgagcgga atttgcgcca gatggtacat atcagcctca atgatacagg
    37321 caatacgcgg gtaaccatcg gtggtctgtg cgtcgttcat caacacaatc ggctgcccgt
    37381 tatgtggcac ctgcaccacg cccggtaaca aaccgtgaga taacagttcg cgatcggtgg
    37441 tgcgttttaa aatttgcccc tgtaagcgat agcccatgcg gttactttgc gagctaagct
    37501 gccagggcga acgccagaat gcatcctgcg aggcgcgatc gaactcatga tattccggcc
    37561 ccggcaaggc gcgaatgcgg ttgccccaca gcagctgttt aacgccctgc gcttccataa
    37621 aatcacgctt cgatttgcca atcgggagtc ggtcaccatc cttcagtaaa cggccttcca
    37681 gcccgccaat ccccactttg agatcggtgc tgcatgaccc cattaccggc ggaacatcaa
    37741 taccacccgc gaccgccaga taactgcgca tcccgtgctg cgggcgttta agcgttaaac
    37801 gctggcctgc tttcatcggc aatcgccagc cggtccagac ggcattatca tccagccgcg
    37861 cttcgcaacc ggcacccgtc agagcaaacc acccatcagt ttcgaactca accgttaact
    37921 gaccgagcgt gatctccagc gcgggggcat tggcgtcatt acccaccagc aggttagcaa
    37981 tgcgtaacgc aggcatatcc agtgcgccgc agtggctgat tcccgactgg cgaaaaccgt
    38041 gacgaccgcc atcctgcaca gtggtataca tgcccgcacg aataatcttc agcatactcc
    38101 ctccttctgc ggtacaaagc gcacgctgtc tcccggacgt aataagatgg gttcgtcacg
    38161 cgccggatca aacaggctga gtgaggtatg accaatcaac tgccagccac ccggcgttgc
    38221 cagcggataa acaccagtct gcggcccgcc gatcccgaca gaacctgccg gaacgagcaa
    38281 gcgcggttca gcgcgccgtg gcgtgtgtag ttgttccggc aaactcccga gatacgggaa
    38341 gcccggttga aaacctaaaa accagaccac gtattccacg gaggagtgca attcaacaac
    38401 ctgtttttcg ctaaacccgc aatgcgccgc gaccaccgcc aaatccggtc cgcctgcacc
    38461 accgtaaacc accggaattt caataaagcg agactcaggt tccagggcct cgctctcctc
    38521 ccaccagcgt tgcaaacgct ctatggcatc cagcgccagc gactcaggat tacgcaaaat
    38581 caccgtgata ttgttcatgc cgggaatggc ttcaaccaca ttcggcatat ccaccagacg
    38641 ctgcgccagt cgccagatcc gtttctggct agccagcgtc accggcggtt ccagttccag
    38701 cactaccgcc gtttcaccta tcagataaca acgcgctcgt tgcactttcg atccctctga
    38761 ttattcgtta tgcaggatta ggaatatcaa taaaggtcac atcaagatcg gtattttcat
    38821 tcagccactc gctcaatgcg cgaataccac cacgttcagt ggcatggtga cctgcagcat
    38881 aaaaatgcaa cccctgctcg cgggctgaat gaatggtctg ttcagaaact tcgccagtaa
    38941 taaaagcatc cacgccaaaa cgcgcggcgc tatcgataaa actttgcccg ccgcccgtgc
    39001 accaggcgac gcgctggacc acctcaggtc cggtatcgcc acaccataac ggcttacgtc
    39061 ccagacgcgc ttcaatccag gaggctagtt ccagtcccgg cacaggcatg gtcagctctc
    39121 cccacggcac caacggctca atttcgccca tgaccgtgat ccccagtaac gccgccagct
    39181 gtgcgttatt gcccagctca ggatgtacgt caagcggcaa atgccagcca tacaggttga
    39241 tatcattcgc cagcaacgtt tttaaacggt tacgcttcat tccgcgaatg accggagact
    39301 ctcctttcca gaagtagcca tgatgcacaa tgaccgcatc cgcgcccaga cgcactgcct
    39361 catcgagcaa agcctggctg gcggtgacac cggtaacaat tttttgcacc gtctctttgc
    39421 cttctacctg caaaccattc ggcgcgtaat cactaatcgc cgcgctgttc agtttttcgt
    39481 taatcagttg ttccagttcg gtgtttttca tcttcgcgcc ttccgtattc tgatagctac
    39541 accgttaaca tagcgttacc tcgcctgttc gtcgatagcc aatcttttga catattaaga
    39601 aacaaacccg acgccaaaag acgcaaattg tttatatcat cacgcttcag ctaatgtata
    39661 tttatgcaat cagacagcat attgcataaa taatcagatc tgtatcaaag ttttaacttt
    39721 aaggaaacac aagcacgccg cgcaactatt acgtgacgtt gcagtcaatt gtggaattat
    39781 tatgaataaa cacgcatcac aaccgcgcac tatttactat gtcgttgcgc tgcaaatctg
    39841 ggaatatttt agcttttacg gcatgcgtgc cctgctgatt ctctatctca ccaatcaact
    39901 aaaatacaac gatactcacg cctacgagtt atttagcgcc tactgttcgc tggtgtatgt
    39961 cacgccaatc ctcggtggct ttttggcgga taaagttctc ggcaatcgca tggcggtgat
    40021 gctgggggcg ttgttgatgg cgatcggtca tgtggtgctg ggtgccagtg agatccatcc
    40081 gtcattcctc tatctgtccc tggcgattat cgtttgtggc tatggtctgt ttaaatccaa
    40141 tgtcagttgc ctgctcggcg agctgtatga gccaaccgat ccgcgtcgtg atggcggttt
    40201 ctcgctgatg tatgcagcgg gtaacgtggg gtctattatc gcacctatcg cctgtggtta
    40261 cgcccaggaa gagtacagct gggcgatggg ctttggcctg gcggcggtgg gtatgatcgc
    40321 gggtctggtc attttcttat gtggcaatcg tcatttcaca catacccgcg gcgttaataa
    40381 aaaagtactg cgtgcaacaa actttctcct gcctaactgg ggatggctgc tggttctgct
    40441 ggtggcaacg cctgcgctga ttaccgtact gttctggaaa gagtggtcgg tatacgcctt
    40501 aattgtcgcg actattattg gcctgggtgt actggcaaaa atttatcgca aagcagaaaa
    40561 ccagaaacag cgtaaggagc tggggctgat tgtgacgctc accttcttca gtatgttgtt
    40621 ctgggccttc gcacaacagg gcggcagctc gattagcctt tatatcgacc gcttcgttaa
    40681 ccgcgatatg tttggttata ccgttccgac tgctatgttc cagtcgatta atgccttcgc
    40741 agttatgcta tgcggtgtgt tcctggcgtg ggtggttaaa gaaagcgtcg caggtaatcg
    40801 taccgtgcgc atctggggaa aatttgctct tggccttggc ctgatgagcg ccggattctg
    40861 cattctgacc ttaagcgccc gcgggtccgc aatgtatggt cactcttctc tgccactgat
    40921 ggtgttaggc ctcgcggtga tgggctttgc ggaactgttt atcgacccgg ttgccatgtc
    40981 gcaaattacg cgcattgaaa ttccgggcgt gaccggcgta ttaaccggca tctacatgct
    41041 gctttctggc gcgatcgcga actatctggc gggcgtgatt gccgatcaga catcgcaggc
    41101 ttcgtttgat gcttccgggg cgatcaacta ctccatcaat gcatatattg aagtatttga
    41161 tcaaattacc tggggcgcac tggcgtgtgt aggactggta ctgatgattt ggctgtatca
    41221 ggcgctgaaa ttcagaaacc gcgcgctggc gctggagtca taataggcca gcaccgatgc
    41281 ctgatgcgac gcttgacgcg tcttatca
//
LOCUS       DAKISA010000030        41115 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.030, whole genome shotgun sequence.
ACCESSION   DAKISA010000030 DAKISA010000000
VERSION     DAKISA010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41115
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.030"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..1710
                     /locus_tag="OVW45_001624"
     CDS             <1..1710
                     /locus_tag="OVW45_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCV5526100.1"
                     /translation="EMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQVQDSISTK
                     LSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLDALVDGPW
                     GGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGARFTWWGED
                     GNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRALVLAWAQ
                     EMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADAVANVLTM
                     TLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAILSGLTAEQ
                     STIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVPDILSFGD
                     ELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSNEIDSDAL
                     YLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQLLEKARNE
                     MGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAAGSEWTGM
                     QVK"
     gene            1711..3360
                     /locus_tag="OVW45_001625"
     CDS             1711..3360
                     /locus_tag="OVW45_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCV5526101.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYTWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3365..4141
                     /locus_tag="OVW45_001626"
     CDS             3365..4141
                     /locus_tag="OVW45_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCV5526102.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGIGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(4215..5399)
                     /gene="atoB"
                     /locus_tag="OVW45_001627"
     CDS             complement(4215..5399)
                     /gene="atoB"
                     /locus_tag="OVW45_001627"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HCV5526103.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKANSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(5430..6752)
                     /locus_tag="OVW45_001628"
     CDS             complement(5430..6752)
                     /locus_tag="OVW45_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HCV5526104.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVTLIVVMPFITRMMMPKPSDVVSIDPE
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMEQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(6749..7399)
                     /gene="atoA"
                     /locus_tag="OVW45_001629"
     CDS             complement(6749..7399)
                     /gene="atoA"
                     /locus_tag="OVW45_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HCV5526105.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRSCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(7399..8061)
                     /gene="atoD"
                     /locus_tag="OVW45_001630"
     CDS             complement(7399..8061)
                     /gene="atoD"
                     /locus_tag="OVW45_001630"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HCV5526106.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(8257..9642)
                     /gene="atoC"
                     /locus_tag="OVW45_001631"
     CDS             complement(8257..9642)
                     /gene="atoC"
                     /locus_tag="OVW45_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HCV5526107.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSASWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTASVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(9639..11465)
                     /gene="atoS"
                     /locus_tag="OVW45_001632"
     CDS             complement(9639..11465)
                     /gene="atoS"
                     /locus_tag="OVW45_001632"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HCV5526108.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKMLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSML
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTADLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPLLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            11632..14481
                     /gene="rcsC"
                     /locus_tag="OVW45_001633"
     CDS             11632..14481
                     /gene="rcsC"
                     /locus_tag="OVW45_001633"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCV5526109.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKALGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(14681..15331)
                     /gene="rcsB"
                     /locus_tag="OVW45_001634"
     CDS             complement(14681..15331)
                     /gene="rcsB"
                     /locus_tag="OVW45_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCV5526110.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(15348..18020)
                     /gene="rcsD"
                     /locus_tag="OVW45_001635"
     CDS             complement(15348..18020)
                     /gene="rcsD"
                     /locus_tag="OVW45_001635"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCV5526111.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDTTATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAAGPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            18758..19864
                     /gene="ompC"
                     /locus_tag="OVW45_001636"
     CDS             18758..19864
                     /gene="ompC"
                     /locus_tag="OVW45_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCV5526112.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGVTQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSS
                     KRTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQN
                     FEAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMST
                     YVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            19976..21031
                     /gene="apbE"
                     /locus_tag="OVW45_001637"
     CDS             19976..21031
                     /gene="apbE"
                     /locus_tag="OVW45_001637"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCV5526113.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGTKTDGAMDITVGPLVNLWGFGPEQQSVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLNVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            21105..22169
                     /gene="ada"
                     /locus_tag="OVW45_001638"
     CDS             21105..22169
                     /gene="ada"
                     /locus_tag="OVW45_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCV5526114.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            22169..22819
                     /gene="alkB"
                     /locus_tag="OVW45_001639"
     CDS             22169..22819
                     /gene="alkB"
                     /locus_tag="OVW45_001639"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCV5526115.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            22895..24538
                     /gene="yojI"
                     /locus_tag="OVW45_001640"
     CDS             22895..24538
                     /gene="yojI"
                     /locus_tag="OVW45_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCV5526116.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAITLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            24756..26402
                     /gene="mqo"
                     /locus_tag="OVW45_001641"
     CDS             24756..26402
                     /gene="mqo"
                     /locus_tag="OVW45_001641"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCV5526117.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVVAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(26711..27199)
                     /gene="eco"
                     /locus_tag="OVW45_001642"
     CDS             complement(26711..27199)
                     /gene="eco"
                     /locus_tag="OVW45_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCV5526118.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLTGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            complement(27334..27498)
                     /locus_tag="OVW45_001643"
     CDS             complement(27334..27498)
                     /locus_tag="OVW45_001643"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5526119.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            27607..28101
                     /gene="napF"
                     /locus_tag="OVW45_001644"
     CDS             27607..28101
                     /gene="napF"
                     /locus_tag="OVW45_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCV5526120.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            28091..28354
                     /gene="napD"
                     /locus_tag="OVW45_001645"
     CDS             28091..28354
                     /gene="napD"
                     /locus_tag="OVW45_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCV5526121.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            28351..30837
                     /gene="napA"
                     /locus_tag="OVW45_001646"
     CDS             28351..30837
                     /gene="napA"
                     /locus_tag="OVW45_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCV5526122.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            30844..31539
                     /gene="napG"
                     /locus_tag="OVW45_001647"
     CDS             30844..31539
                     /gene="napG"
                     /locus_tag="OVW45_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCV5526123.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            31526..32389
                     /gene="napH"
                     /locus_tag="OVW45_001648"
     CDS             31526..32389
                     /gene="napH"
                     /locus_tag="OVW45_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCV5526124.1"
                     /translation="MANRKRDAGREALVKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSCSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            32386..32835
                     /gene="napB"
                     /locus_tag="OVW45_001649"
     CDS             32386..32835
                     /gene="napB"
                     /locus_tag="OVW45_001649"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCV5526125.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            32845..33447
                     /gene="napC"
                     /locus_tag="OVW45_001650"
     CDS             32845..33447
                     /gene="napC"
                     /locus_tag="OVW45_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCV5526126.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            33466..34083
                     /gene="ccmA"
                     /locus_tag="OVW45_001651"
     CDS             33466..34083
                     /gene="ccmA"
                     /locus_tag="OVW45_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCV5526127.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFDDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            34080..34742
                     /gene="ccmB"
                     /locus_tag="OVW45_001652"
     CDS             34080..34742
                     /gene="ccmB"
                     /locus_tag="OVW45_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCV5526128.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            34784..35521
                     /gene="ccmC"
                     /locus_tag="OVW45_001653"
     CDS             34784..35521
                     /gene="ccmC"
                     /locus_tag="OVW45_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCV5526129.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            35518..35727
                     /gene="ccmD"
                     /locus_tag="OVW45_001654"
     CDS             35518..35727
                     /gene="ccmD"
                     /locus_tag="OVW45_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCV5526130.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            35724..36203
                     /gene="ccmE"
                     /locus_tag="OVW45_001655"
     CDS             35724..36203
                     /gene="ccmE"
                     /locus_tag="OVW45_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCV5526131.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMLGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            36200..38143
                     /gene="ccmF"
                     /locus_tag="OVW45_001656"
     CDS             36200..38143
                     /gene="ccmF"
                     /locus_tag="OVW45_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCV5526132.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMIASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASYSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            38140..38697
                     /gene="dsbE"
                     /locus_tag="OVW45_001657"
     CDS             38140..38697
                     /gene="dsbE"
                     /locus_tag="OVW45_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCV5526133.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            38694..39746
                     /gene="ccmH"
                     /locus_tag="OVW45_001658"
     CDS             38694..39746
                     /gene="ccmH"
                     /locus_tag="OVW45_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCV5526134.1"
                     /translation="MRFLLGMLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIDGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(39781..40428)
                     /gene="narP"
                     /locus_tag="OVW45_001659"
     CDS             complement(39781..40428)
                     /gene="narP"
                     /locus_tag="OVW45_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCV5526135.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            40748..>41115
                     /locus_tag="OVW45_001660"
     CDS             40748..>41115
                     /locus_tag="OVW45_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCV5526136.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVGLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAVNQTTLNNGVLQVYG"
BASE COUNT         9583 a        10208 c        11294 g        10030 t
ORIGIN      
        1 gagatgcgcc agacgctgac gctgcacaaa ggggcgaatt atatttcgct ggcgcagaac
       61 attcagcaat ctggcttgtt aagcgcagaa ctgcaacaaa atgggcaagt gcaggacagc
      121 attagcacaa aactgtcttt tgtggataac agctggcccg ttgaacagca gaaaaatgtc
      181 atgctcggcg gtggcgagaa cgcgctgatg ttgcccgagc aggctagcaa tatccggcta
      241 caaagtagcg aaacgccgca ggagattttc cgcaacaatc ttgatgcgtt agtcgatgga
      301 ccatggggtg gcgtgatcaa caccggtagc cgtctgatcc cgctcagtct cgcctggcgt
      361 tcgcttgccg atcatcaaag tgccgccgct aacgacattc gtcagatgat tcaggataac
      421 cgtctgcggc tgatgcaact ggcggggccc ggagcgcgct ttacctggtg gggtgaagat
      481 ggcaatggtg acgccttcct tacggcatgg gcatggtacg ccgactggca ggccagccag
      541 gcgctcggcg taacgcaaca accggaatac tggcagcata tgctcgacag ttatgccgag
      601 caggcggata acatgccgtt attgcatcgg gcgctggtgc tggcgtgggc acaggagatg
      661 aatctgccgt gcaaaacgtt gttgaaaggg ttggatgaag ctatcgcccg gcgcggaact
      721 aaagatgaag atttctctga ggaagacatc cgcgatatca atgacagcct gatcctcgat
      781 acaccggaat ctccactggc agatgcggtg gcaaacgtct taaccatgac gttgctgaaa
      841 aaagcgcagt tgaagtccac ggtgatgcca caggttcagc aatatgcgtg ggataaagcg
      901 gcaaacagca atcagccgct ggcgcacacg gttgtgctgc tcaatagcgg tggtgacgct
      961 acccagacgg ccgctatttt aagtggtttg accgctgagc aatccactat tgagcgcgcg
     1021 ctggccatga actggctggc gaaatatatg gcgacaatgc ctccagttgt tttgcctgcg
     1081 cctgcgggcg catgggctaa acataagtta actggagggg gcgaagactg gcgttgggtt
     1141 ggtcagggtg tgccggacat tctctctttt ggtgacgaat tatcgccgca aaatgtgcag
     1201 gtccgctggc gtgaaccggc aaaaacggct caacaaagta acattccggt gaccgttgaa
     1261 cgccagttgt atcggcttat ccccggtgaa gaagagatga gctttactct gcagccggtg
     1321 accagcaatg agattgacag cgatgcgctg tatctcgatg aaattacgct taccagcgag
     1381 caggatgcag ttctgcgcta tggtcaggtg gaagtaccgc tcccgccggg agccgacgtt
     1441 gagcgcacaa catggggcat ttcagtcaat aaacccaacg ccgcgaaaca gcaggggcaa
     1501 ttgctggaaa aagcgcgtaa tgaaatgggc gaactggcct atatggtgcc ggtgaaagaa
     1561 ctgacgggaa cggtcacttt ccgccatttg ctgcgcttct cgcaaaaagg gcaattcgtt
     1621 ctgcctcctg ctcgttatgt gcgttcctat gcacctgcgc agcaaagtgt tgcggcaggg
     1681 agtgaatgga ccgggatgca ggtgaaataa gtgaactggc gcagaatcgt ctggctattg
     1741 gcgttggtta ctctgccaac gcttgcggag gagacgccgt tacaactggt gctgcgcggt
     1801 gcgcagcacg atcaactgta tcagctgtcg tcatcaggcg tgaccaaagt ctcagcatta
     1861 cctgactcgc tcaccacgcc gttagggagt ttgtggaaac tgtatgttta cacctggctg
     1921 gaggataccc atcaaccgga gcagccctac cagtgtcgcg gaaactcccc ggaagaggtt
     1981 tattgttgtc aggctgggga gagcataacg cgcgataccg cgctggtacg ttcctgtggg
     2041 ctctattttg cgccgcagcg gttgcatatt ggcgcagatg tgtgggggca atactggcaa
     2101 cagcgtcagg cacctgcgtg gttagcgtct cttacaacgc tgaagccaga aacctcggta
     2161 acggttaaat cgctgctcga ttcattagcg acgttacctg cgcaaaacaa agctcaggaa
     2221 gtcttactgg atgtggtgct ggacgaggcg aaaatcggtg tcgcatccat gctcggtagt
     2281 cgggtacggg tgaaaacctg gagctggttt gccgatgata aacaggaaat ccgccagggt
     2341 ggttttgctg gctggttaac cgatggtaca ccgctgtggg ttactggcag tggcaccagc
     2401 aaaacggtac taacccgtta tgcgacggtg cttaatcggg ttttgccagt gccgacgcag
     2461 gttgccagtg ggcaatgtgt agaggtggag ctgtttgccc gctatccgct gaagaaaatc
     2521 acagcggaaa agagtacgac ggcagtaaaa cccggcgtac ttaatgggcg ctatcgtgtg
     2581 acgtttacta atggtaacca cattacgttt gtcagccacg gtgagacgac cttactgagc
     2641 gaaaagggca aactgaaatt gcagtcgcat ctcgatcgtg aagagtacgt tgccagagtt
     2701 ctggatcgcg aggcgaaaag tacgccacct gaggcagcga aagccatgac cgtggcgata
     2761 cgtacttttt tacagcaaaa cgccaatcgg gaaggtgatt gcctgaccat ccccgacagc
     2821 agcgccacgc agcgtgtttc tgcttcgcca gcgaccacgg gggcgcgaac gatgaccgct
     2881 tggacgcagg atctcatcta tgcaggcgat ccggttcatt atcatggcag tcgcgctacc
     2941 gaagggacac tttcctggcg gcaagccacg gcccaggcgg ggcaggggga gcgttacgac
     3001 cagatactcg cgtttgctta tcccgacaac agccttagcc gctggggagc tccacgctca
     3061 acctgccagt tattacccaa agcgaaagcc tggctggcga aaaaaatgcc gcagtggcgg
     3121 cgtatattac aaggcgagac ggggtacaac gaaccagacg tgtttgcggt ctgtcgttta
     3181 gtctcaggtt tcccctatac cgatcggcag cagaaacggc tgtttattcg taacttcttt
     3241 acgcttcagg atcggctgga tttaacccat gagtatctgc acctggcctt tgatggttat
     3301 cccaccgggc ttgatgagaa ctatatcgaa acgctgaccc gacaattatt aatggactaa
     3361 tgctatgcga aagatttttc ttccgttgtt actggtggca ctttcgcctg tcgctcacag
     3421 tgaaggtgtg caggaagtcg agattgacgc accgctctct ggctggcatc cggcagaggg
     3481 cgaagatgcc agtttttcac aatcgatcaa ttacccggcg tcgtccgtca acatggcaga
     3541 tgatcaaaat atctcagcgc agatccgcgg caaaataaag aattatgctg cggcgggtaa
     3601 agttcagcag ggccggctgg tggtcaacgg tgccagtatg ccgcagagaa ttgaatccga
     3661 tggttcattt gcacgccctt atattttcac tgaaggcagc aacagcgtgc aggtcatcag
     3721 cccggatggg caaagccggc aaaaaatgca gttttactca acgccgggta taggaacgat
     3781 tcgtgcacgt ttacggctgg ttctctcgtg ggatacggac aataccgatc tcgatctcca
     3841 tgttgtcacg cccgatggcg aacacgcctg gtacggtaac accgtgctga aaaacagtgg
     3901 tgcactggat atggatgtca cgacggggta cggacccgag attttcgcca tgccagcacc
     3961 agttcacggg cgctatcagg tgtatatcaa ctactatggt gggcgcagcg aaacggaatt
     4021 gacgaccgcc caactgacgc tgatcaccga tgaaggatcg gtcaatgaga aacaggaaac
     4081 atttattgta ccgatgcgta atgctggcga gctgacgctg gtgaaaagtt ttgactggtg
     4141 attaatgggg ttaccttcgc cgtcgatgtt caagaaaacg cccgataact ttcgctatcg
     4201 ggtgttttta ttgattaatt caaccgttca atcaccatcg caattccctg gccgccacca
     4261 atgcacagtg ttgccagccc cagcgtttta tcgcgtgcct gcatggcatg taatagtgtg
     4321 accagaatac gagcaccact ggcaccgata ggatgcccga gcgcgatggc cccgccgttg
     4381 acattcactt tctcagaatc aaagcccagg gttttcccaa cggcgaggaa ctgtgcagca
     4441 aatgcttcat tagcttcgat gagatcaatg tctgccagtt gcagccccgc cagttgtaac
     4501 gctttttgcg tggcaggtac tggccccata cccatcaatg cgggaggcac gccaccgctg
     4561 gcataacttt taatgcgagc caggggggta aggcctgctg ccagcgccgc agattcttcc
     4621 ataatcacca gagcggcagc accgtcgtta ataccagacg cgttcccagc ggtgactgtt
     4681 cctgctttat cgaaggccgg gcgcaatgca cctaacgctt cagccgttga attcgctttc
     4741 gggaattcgt cttgactgaa gacgaaggtt ttcttccgag tgacaacatt taccgggacg
     4801 atttcggctg taaaagcacc ggactcaatt gcggctgccg ctttacgctg tgaatgtagc
     4861 gccagttcat cctgcatttc acgggtaatt ccgtactctt tagccacgtt ttcggcggta
     4921 atccccatat gataaccatg ggtggcgcac atcaggccat cgcgcaggat tacgtcataa
     4981 acctgtccgt ctccaagacg ataaccagag cgtgcttttg catcgagtaa gtagggcgct
     5041 aaactcatat tttccatacc ccccgccaca atgctctgcg cctgacctgc ctgaattgcc
     5101 tgggcggcaa gcgccacact tttaagaccc gaaccacaca ctttattgac cgtgaatccg
     5161 cacaccgttt ctgccagccc gctttttaac agtgcctgac gtgccggatt ttgccccaac
     5221 ccggcttgca acacgttacc cataatcact tcatcaatgt gtagtgaatc gatttttgca
     5281 cgttcaatgg cggctttaat tactgtcgcc cccaggtcga tggcgctggt ggaagcgagt
     5341 gaaccgttaa aactaccgat agcagtacgt accgcactga cgatgacaca atttttcatt
     5401 ttatattcct tctgtctgta gggttgccgt cagaacagcg ttaaaccaat gacgaaaatg
     5461 acgccggaga agagcagggc agtgatgcaa tagcccatga tgtcgcggac accgagtccg
     5521 gcgattgcca gtgctggcag cgcccagaat ggttgttcca tgttcatcca ttgctcgccg
     5581 taggcgatcg ccattaccga tttaccgaga tcagcgccca gcgcctgggc tgcgggtatc
     5641 acgaaaggtc cctgaataac ccagtgaccg ccgccagacg gaacggcgaa gttaatcagt
     5701 gcagaactaa aaaaggtcat taccgggaag gtgtctttgt tcgcaacatt gatgaagaat
     5761 tcggtaatga gtccacccag accagaatgc tccatcatca gttggatccc agcgtagaag
     5821 gggaattgca ccagaatacc ggcagtactg cgtgctgccg cgctgatagc acgcatataa
     5881 gccattggcg ttttatgtag caacagaccc gcaatcataa acatcaggtt gacggtattg
     5941 atggtgatgt tgaagccatg ttcgctgaag tacatcgcca ggtaagcgat accgagtgcg
     6001 ccgatgatca acgtcagaat gcggctttct tccagtcgct cggatggtgg ggcatctttc
     6061 ggtagctgct tttgaaaatc agcctcttcc atgagtagtt ccggatcgat actcaccacg
     6121 tcagacggtt ttggcatcat catgcgggtg ataaatggca tcaccacaat caacgtcaca
     6181 gtgatgaaaa tgttaaaacc actgaacaga gtatcgccca ccgggatcag cccggcgata
     6241 tgctcaaccg ggttgcccgg tgttgcagcc aacagaggca ttgatccaga gaagccgcca
     6301 ccccaggtga gaaaaccaat gtaggcgcag gcaatgagca acggataatc agaaccgggg
     6361 actcgccggg cgacttcacg ggcaaacatt gcgccgacaa ccaaaccaaa tccccagttg
     6421 atgacacaag cgactgaacc gaagaaagtg accagcatga cgccctgtac gggcgtcttt
     6481 gcggcggagg cggcagtacg cagcaaactt ttcaccggag cagagctggc aagggcatga
     6541 ccggtaacga tgataagcgc catctgcata ccaaacgcca gcaagttcca gaaaccgtca
     6601 ccccacattt tcaccatgct gatcggcgtt tgtggtgtta accaaagcgc gatcacgaat
     6661 gttagcaatg tcagcaacat ggcaaagatc agtggatcgg gaagccatcg gctgacaaaa
     6721 cgcgtcataa aacgcgatat gcgaccaatc ataaatcacc ccgttgcgta ttcagatcgg
     6781 cggcgacttc aaaccgagct tctgttttgg cacgcacggt ggctaaatca cacccgtcgg
     6841 caatttcggt gagccacatt ttgccgtcaa taaaacgaaa gacagccagt tcagtaacca
     6901 gcatatgcac cgcatgttgc gcagtgagtg gcatggtgca gctgcgcaaa atttttgctg
     6961 aaccatcttt ggcgcaatgt tccatggcga tgatcacttt gcgcgacccg gtcaccagat
     7021 ccatcgcgcc acccataccg ggcaccattt tcccaggcac tacccagttc gcgaggtttg
     7081 cttcttcgtc tacttgcaaa ccgccgagca cgcaggcatc aatatgaccg ccacggatta
     7141 gcgcaaatga catggcgcta tcaaacatgg ctgcaccggg taaaacaccg cacggttgcc
     7201 cgccagcgtt caccagatct ggatgcgctg tcgtgaccgg gcctaaaccg aggaagccgt
     7261 tttccgattg cagagtgata tgaataccct ccggtaaata attggcgacc attgtgggta
     7321 aaccgatccc taagttaacg atgtcaccat cacgaagctc ttgcgccaca cggcgcgcaa
     7381 tacgttgttt cgcatccatt atttgctctc ctgtgaaacg atgatgtggt cgataacggc
     7441 accaggggtg acaatatggt caggttgcag ctcgccggtt tcgaccagtt catctggctc
     7501 taccagcgtg atatcagccg caagggctat cagggggtta aagttgcggg cgctaagttg
     7561 ataggtcagg ttgccaagtg tgtcgcaacg atgagcgcga attagcgcca ggtcggcgcg
     7621 cagtgggcgt tcgagcagcc aggttttacc gtcgagtgtc agtgtctgtt tgccttcctc
     7681 tacgacggtg ccgacacccg ttggggtgag aaaaccacca agtccagctc caccacagcg
     7741 aatttgctcg attagcgtac cttgcggcac cagaacgacg tccatctcac cagatatcat
     7801 gcgccgacct gtttccgggt tggtgccgat atgtgaagca atcactttgc ggactcgacc
     7861 attgacgatg agcggaccga tgccggtatc aacaaacgcg gtatcattgg ctatcaatgt
     7921 caggtcgcga acaccagatt ccagtaatgc ttcaaccagg cgggatggag tgccaatccc
     7981 cataaatccg cccaccatga tggtcatgcc gtcacgaaag aagccggtgg cgtcttgtaa
     8041 tgtcatcaat tttgttttca ttttttatcc ctcttgcata caccgttgtg ggtacagtca
     8101 ggcaatagca aggggagtgc cagaatgaga ttattggatt tagttattta actaattgaa
     8161 tatgaaggga taaattggat ggggatttat aaggagaatg agcggctatg cagaaaattg
     8221 cgcactgtgc aaatttctgc atagcaagtt ttggtgttat acatccgccg gatcgatacc
     8281 gtattcctgg agtttataca tcaatgcacg gcgactgatg cccagcatta aagcagtgcg
     8341 ggttcggttt ccttcttgtt gttccagcac ttccataatg atgcgttttt cgacgcgttt
     8401 aatttcctct tttaaattac gctcaccgac agaggctgtt tttacctcgc cagcattaca
     8461 gactggctga cgaatctgtg gcggaagatc ctcagaaaaa atgatcgggc ctgaattcat
     8521 cacgacggcg cgttcaataa cgttagaaag ctctcgaata tttcccggcc atgaccaggc
     8581 ggtaagcagt gacattgcca tcggatcgat gtcgataata tcgcgctgat tctcactact
     8641 gaatttttgc aaaaagtgat tagctaacag ggaaatatct tcccggcgat cgcgcagagg
     8701 cggcagtatt aaatgaataa cgttaaggcg ataaaagaga tcttcacgga aggtgccttc
     8761 ttttaccatt gcctgcaagt cacggttggt ggcagcaatg atgcggatat caacttttat
     8821 ggtctgatgg ccgccaatcc gttcaaattc ccgttcctgt agaatgcgta gtaatttggc
     8881 ttgtagtacc agcggcattt cgccaatttc gtcgaggagc agagtacctt cgttggctcg
     8941 ttcaaataat ccctgacgca aggtttgtgc accagtaaat gcaccttttt catgaccaaa
     9001 cagttcactt tcgagcaacg attccggcag cgccgcgcag ttgactttaa tgaacggccc
     9061 ctttgcccgc cgcgaattgt agtgaatcgc tctggcaatc aactctttcc cggtgccgct
     9121 ttcaccgcta atcaagacgc tggcctgaga aagggcaatt ttggcggtgt ctttgcagat
     9181 gtccatcatc gccgggctgt tggtgagaat gtgcccccat tgccagctgg cgctcagtgc
     9241 ctggtgcaga tgacggatct cttttttcat tgactggagt tgtaaagcgc gctgaacgat
     9301 taaattcaac tcatcgagat caaacggttt aataacatag tcgaaggctc cgcagcgtag
     9361 cgcttcgacg gcggtttcca cttccgcata ggccgtcatc agaataacgg gtgtccgggt
     9421 ctcatggctg cgcatctcct ttagtgcctt gatgccgtcc atctctggca tgcggatatc
     9481 catcaacacc acatcagggt gaatatcggc aaacaggtgt aatgctgtgc gtccgttgtt
     9541 cgcacaatgt gtttcgaatc cttgtagtgc aaaagcggtg ctcagcatac ggcgaacatt
     9601 atcttcatca tccacaataa ggatgcgatt aatagcagtc atacagtctg atttccctgc
     9661 gggttgatcg gtaaaataag cgtgaaggtt gcgccgtagc ccggcaaact ggcgacgcga
     9721 atatcaccct gatgggcatt aatgatgcgt tgacttaacg ccagaccaag cccggttcct
     9781 gaggctttgg tggtgaaaaa gggatcgaag atcttttttt gcagcgagag atcaatgcca
     9841 cagccgttgt cctctatcga aatggcctgt tgtgagtcgc tgtattgcca ggtttgaatg
     9901 cgaattttcc ctcgtgcgct gatagcctgg acggcattga tcaggatatt cagtagtacc
     9961 tgtttgagca gttcacgatc ggcgttaatc gggcttaatt cattatccag ttcgcttatg
    10021 aagtcgaccc gcgcttgtac gccggcggtt tgtaccagta ccagagtttc ttcaaccaat
    10081 gcattgaggc tgacttgttg ccattgactg tggcgtggac gtgaaaattc gagcaattgc
    10141 tgaataactt tgttaattga atcgatttct ttgagtacta cggacagata ttcctgatgt
    10201 attgggtcac tggtttgttg gcgcaagatc tgtacataac cacgaatagc cgttaacgga
    10261 ttacgtactt catgcgcgac gccagccatc agctcaccca gtgtggcgag gcgttctgct
    10321 tgcgccatgc ggcgctgggt ttctttgcgg gcagttaaat cagagaaaat caccaaagca
    10381 cctatcattt caccgtgcgt gttatgaata cgactggtag tgacactgag ttcaatggtg
    10441 cggtcacgac ctggaaaact gatctccagc gccacatgtt cggtgccatg ttccagcgta
    10501 tccagtactg gactgtagaa ctgagtattg tcgaacaaca tggagtaagg ctgccctacc
    10561 agttcatggc gttgatagcc agtgataact tctgctgctg ggttcatggt ggttacatca
    10621 ccctggcggt caatggcaat gacgccatcg gcagcgtttt caataatcag atcgttaagt
    10681 gtccgcgttt cacgcagtgc ctgggcgagg ttattaacac tctgactgat ttgccccatt
    10741 tcaccgggca atagtggtaa tcgagtggga atattttgtg ccagagtcga gaggccatcg
    10801 gtgatgatat caatattggc gctcaggcga cgggagaaaa ggacaatcaa tagcaggctt
    10861 atcagcaaat cggcggtgag cacaatgata atcctcacat ccattttcca ggcctggcgg
    10921 cgaatatctt cggttaattc attggcccag atatagccga ggatttcacc attacgctca
    10981 atggggagca ttgaattcaa aatatcgccg cgcacctgcc tgcctgaata aaccaaaggg
    11041 gtatttgtac gcatgacttc gcgaccaggg tgatctgcgg caatggtaac gccgacatta
    11101 ttctgatata gcgctgaagg cgcgtaggtg attatcgcat ccagcatttt gttgtaataa
    11161 ccagcaccta tgccagggaa ggcgtgagtg atattttcgg taatgggggc aagttctgca
    11221 tttaatgcgc ggatacgctc ctcacgtggt aagtcgatgt agagatcata gcgatcgcct
    11281 agtgcctgat taagcaggtt gaccacggca gaaagttttt tctctttttc agataagact
    11341 gctgaacgtc cttccgtttc tacgatataa ccaatagtaa gcgttgggac aatgaccatc
    11401 aggattgcca tcaggatcat ttgattgcgt aagcggcgtg gataaatcca cttcatataa
    11461 tgcatgctga aatccactag tcttgtccgg tatatgacga ttatcagagg ttaaggtgat
    11521 gatttctcgg cggtgtatca tattccagag aagagagaac attgcggtaa cacgctttta
    11581 ccgctacctt aaccacactc catcggtcac ctgaggcgga gcttcgcccc tttgaaatac
    11641 cttgcttctt ttcgtacaac cctgaaagcc tcgcgctaca tgttcagagc attggcgtta
    11701 gtgctctggc tgttgattgc tttttcatcc gttttttaca tcgttaatgc gttacatcag
    11761 cgagaatcgg aaattcgtca ggaatttaat ctgagttccg atcaggctca gcgctttatt
    11821 caacgcacct ctgatgtgat gaaagagctg aagtacatcg ccgaaaatcg cttatcggca
    11881 gaaaacggtg tgctttcccc gcgtggacga gaaacgcagg cggatgtgcc tgcgtttgaa
    11941 ccgctgtttg ccgactccga ttgttccgca atgagtaaca cctggcgagg ttctctggag
    12001 tcattggcgt ggtttatgcg ctactggcgc gataattttt ctgcggctta cgatctaaac
    12061 cgggtatttt taatcggcag cgataacctc tgcatggcca atttcggtct gcgtgatatg
    12121 ccagtggaac gcgataccgc gttgaaagct ttgcatgaac gcatcaataa atatcgaaat
    12181 gcaccacaag atgatagcgg cagtaacctc tactggatca gcgaaggtcc gcgccctggc
    12241 gtcgggtatt tttacgcgtt gacgccagtt tatctggcga accggttgca ggcgcttttg
    12301 ggtgtcgagc agaccatccg gatggagaac tttttcttac cgggtacgtt gccgatgggg
    12361 gttaccattc ttgatgaaaa tggtcatacc ctgatttcgc ttaccggacc agaaagtaaa
    12421 attaagggcg atcctcgctg gatgcaggaa cgctcctggt ttggctatac ggaagggttc
    12481 cgggagctgg tgctgaagaa aaatctgcca ccctcatcgc taagcatcgt gtattcggtg
    12541 ccggttgata aggtgctgga acgcattcgc atgttgatcc ttaacgcaat tttgctgaat
    12601 gtgcttgccg gagctgcatt gtttactctc gcacggatgt acgagcgacg tattttcatt
    12661 ccggcggaaa gcgacgccct gcgactggaa gaacatgagc agttcaatcg caagattgtc
    12721 gcctccgcgc cagtgggtat ctgcattttg cgtaccgctg atggcgtcaa tattttaagt
    12781 aacgaactgg cgcataccta tctcaatatg cttacgcatg aggaccgcca acgactgacg
    12841 caaattatct gtgggcagca ggtcaatttt gttgatgtcc tgaccagcaa caataccaat
    12901 ctgcaaatca gcttcgtcca ttcgcgctat cgtaatgaaa acgtggccat ttgtgtgctg
    12961 gtggatgttt cttcgcgcgt gaagatggaa gagtcgttgc aggagatggc acaagcagcg
    13021 gaacaggcga gccagtcaaa atcgatgttc cttgccaccg tcagtcatga gctgcgaacg
    13081 ccgctgtatg gcattatcgg taacctggat ctgttgcaaa ccaaagagtt accgaaaggc
    13141 gtcgatcggc tggtgacggc aatgaacaac tcttccagcc tgttgttgaa aattatcagc
    13201 gatattctcg atttctcgaa gattgaatcg gaacagttga agatcgaacc gcgtgagttt
    13261 tcaccgcgtg aagtgatgaa ccacatcacc gccaactatt taccgctggt ggtacgcaag
    13321 cagttaggct tgtactgctt tattgaaccg gatgtgccag tggccttaaa tggcgacccg
    13381 atgcgtttac agcaggtcat ctccaacctg ttgagtaacg ccataaaatt caccgatacc
    13441 ggctgtatag ttttgcatgt ccgcgctgat ggcgattatc tctctatccg tgttcgcgat
    13501 accggcgtgg ggattccggc gaaagaagtg gtgcgcttgt ttgatccctt cttccaggtc
    13561 ggaacgggcg tacagcgaaa cttccagggg accggtctgg gtctggcgat ttgtgaaaaa
    13621 ctgatcagca tgatggacgg cgatatctcg gtagattcag aaccgggaat gggcagccag
    13681 tttaccgtgc gtattccgtt gtacggcgct cagtacccgc agaaaaaagg cgtggaaggg
    13741 ttgagtggta aacgctgctg gctggcggtc cgcaatgcgt cgctctgtca gttcctggaa
    13801 accagtttgc agcgcagcgg catcgtcgtt acaacatacg aagggcagga accgactccc
    13861 gaagatgtgt taattactga cgaggtagtg agtaaaaaat ggcagggtag agcggtagtg
    13921 accttctgtc gtcgccatat tggtattccg ctggagaaag cgctagggga gtgggtacac
    13981 agtgtggctg ccccgcatga gctaccggca ttgttggcgc gtatttattt gatcgagatg
    14041 gagagcgacg atcctgctaa cgctctgccg tcgacggaca aagcggtcag cgataatgac
    14101 gatatgatga ttctggtcgt ggatgatcat ccgattaacc gacgtctgct ggcagatcag
    14161 ttgggatcgt tgggctatca atgtaaaacc gcgaatgatg gtgtcgatgc gcttaatgta
    14221 cttagcaaga atcatattga tatcgtgctt agcgacgtca acatgccaaa tatggatggt
    14281 taccgcttga cgcaacgcat tcgtcagttg ggactgacgt tgccggtaat cggagtaact
    14341 gctaatgcgt tggctgaaga gaagcagcgg tgtctggagt ccggtatgga cagctgcctg
    14401 tcgaagccgg taacgctgga tgtgataaaa cagacgctga cggtatatgc cgagagggtc
    14461 aggaaatcgc gggaatcgta ggacggataa ggtgtttatg ccgcatctgg cattcagtgc
    14521 aaatgccaga tgcgacgctg acgcgtctta tctggcctac tttgatgtaa tctgttgaaa
    14581 taatgggaat cgtaggacgc ataaggcgtt tacgccgcat tcggcaatcg gtgcaaatgc
    14641 cagataagac actaacgcgt cttatctggc ctacaggtga ttagtcttta tctgccggac
    14701 ttaacgtcac tgaagagaga taattcagca gggcgatatc gttctcgaca cccagcttca
    14761 tcatcgcaga tttcttctgg ctactgatgg ttttaatact gcggttcagc tttttagcga
    14821 tctcggtcac caggaagcct tccgcaaaca ggcgcagaac ttcactctct tttggcgaga
    14881 gacgcttgtc accgtaacca ccagcactga ttttttccaa caggcgagaa acgctttccg
    14941 gggtaaattt cttccctttc tgcagcgcgg cgagagcttt cggcagatcg gtcggtgcac
    15001 cttgtttcag cacgatccct tcgatatcca gatccaatac cgcactaaga atcgccgggt
    15061 tgttgttcat agtcagaaca atgatcgaca ggcttgggaa atggcgcttg atgtacttga
    15121 ttaaggtaat gccatcgccg tacttatcgc caggcatgga gagatcggta atcaacacat
    15181 gcgcatccag tttcggcagg ttgttgatca gtgctgtaga gtcttcaaat tcgccgacaa
    15241 cattcaccca ctcaatttgc tcaagtgatt tgcgaatacc gaacaagact atcggatggt
    15301 catcggcaat aattacgttc atattgttca tgtaataggc taccttgcta cagcaagctc
    15361 ttgacataac tgtcaatgtc gctgatgtat ttttctattc ctggaacatc cttctcacga
    15421 atcagatgtt ccagcgtttc acataactgc ttgccgggta ccagatttag catggcaaat
    15481 acgcctttaa gacgatgagc cgtttgggct aacgcagcaa agtcactggt tgctgcttca
    15541 gtatacagcc tcttaacatc atccggtact gtgtctacaa agagcgcata atagccgctg
    15601 gcatggagtt gcgcattttc atctccgccc agaggcgatt ctgtcacctc ttcctgcgcc
    15661 agttgcactt caattaattg taagaccgct tcctgcatag cgttgctcat attgaagttg
    15721 acgcacaatt gaccaggccc aatttcccgt acgccagact catcatcgct taaaagcaag
    15781 ccagaggcag taagattaga cggattatcc gttaaaaaga tatcataatc ttgactaatt
    15841 aatctttcat cgggtgtgat acaggttgca ccccaatttt ctaactggcg agtgacaata
    15901 ttccgaattt ctgccgaagt aacatccacc attacgcaga catcatccag taaacgctct
    15961 tcttcctctt caacttccgg gccagcggcg agcattttga tatgcacaga gtagcgtgta
    16021 ccaagcccat cccgcgtttt gatgtttaaa tgaccgccca gtttacgtgc cagttgatcg
    16081 ctcagccaga atgccagcgg gtccgccttt ccataacgat cgttttgggt ctggttgata
    16141 aacgggaagt gcaaattatc catttcatga atacttacgc cttctccggt gtccagaatg
    16201 cggaatgtca ggcggtcttc agaggactca tcctgatcaa cctcaagggt gatttttccc
    16261 aattgcgttg aggtcacggc atattgcatc agtagcagca aaatacgtcg taaggcatcg
    16321 cgatcgccgc ggcgcatatc gtgtgctttc agatgattgt taatcagcag ttgcagacct
    16381 ttacgcttga tggcaggcaa cactgaaggc acaacttcat caattaaatc ctgcacggag
    16441 aacagcaccg tctcactttt ccagctatcg tccgcaagca tgttcgctaa ctgtatttca
    16501 tcgaccagcc gcaccagcac atctgcctga ttcgccagtt gtttgctttc cggggcgttg
    16561 agtttagccg cgctctccgc cagggactgt gcaggttctt tcagcgcatc gccaatgttt
    16621 ttcataaagg tcatccggcc ctgctggttt ttctcataca gacgctgcgc ctgcttgagt
    16681 ttcttgttta ccagcacttc gcgatcctga tcgcgaataa tgaaaatttg tgtgcgcggc
    16741 gcgacctggc tgcggaacat gcggatctca tacagctcgt tattgatcgt cgcctgaata
    16801 atcccctgat gctgttccgc catggtggtg atgttttgca gattcaaatg cggcagcaaa
    16861 tgatcggcaa ttttgttact tatgacagtg cggttcgatt cctgatcgtg aaccagcagg
    16921 ccgagcggca gcagtgagac tatctcttca ttgattgccc gtaaaatgcg caattcgtta
    16981 ttgaccgccg tgttgggtag actttctgta ctgcgactgg agaaatggcg gaatgtggta
    17041 tagccaaata acgccagcgc cagcaaaccg atgttcagca gcagtggcag cagaatgttt
    17101 tgcaacgtat ccagcaataa ggtgccataa ggaacctgcc agaccaggcg catatcggta
    17161 gagttgagtg ccgaggagat ttcaatcttc gtactgttaa agtggatact gacgctatcc
    17221 gttccttctt tctcattatc attgtctccc gttgccgtcg tgtctggctc aaggcggaaa
    17281 ctgtccagcg gcatacccgg tgggatcaaa tcattaatcg gcagatcaaa agccacgacc
    17341 gttgccagat gtcccggctg gttgaacgtg gtacgcaagg taaagtaatg accgttctgc
    17401 caggccaggc ggcgcatgtt agaaaaactt tcgcgttcat cgagggcgtt ggcctgttgc
    17461 aacatttctg cacgacgtga atcaacaatg tcactgacgg tcgattcttt aaatccggag
    17521 gtgagatctt tgaggggcag ggttgagatc agtaccagac tgttatcctg accattcagg
    17581 taatacatcg accacggtac attttctgca ccccacaatg tatccagata agtggacatc
    17641 cgctgagtca tctcaagcgt tgagttgtcg tgagagccaa agattaacgc ttccgttttg
    17701 cggcgtggtt tctccagata gtagacatcc tgtttcaggc gcgtctcttg taaaccttcg
    17761 ccggaggagg gggaggtcgt cgcggcaatg ttgtcgtaga tctgccaggt cacgtaacgc
    17821 caggtatcga cgcgcttttg gattgcgtgg gtaatgtcga caatctggta acttttatct
    17881 ttcagccagg cgttaacggc gctttgtacc attaccccca tcgtcaccag taacacaatg
    17941 atcaacagta aaaagaagcg ggtaatgctc cccggtagga gtgaaaagcg ggtcgtggcc
    18001 gttgtctctt tctgacgcat tcgtgtttat gacctgttaa aacttcgcga agtgataggg
    18061 cagtataaag ggtacagtgt gaaattccag actcttacct gattgatgat tctctattct
    18121 cccgtgatat ttatcatcct gcattggggg aaatagatgt acaagcgcca ttttgctgta
    18181 caaataacct acaaaaagcc caacgaaatt aattgcaaat aaacgaagat tgctggaaat
    18241 tatgctgatg ttaattattt gtgaaatagt taacaagagt tatagttttt ctgtggtagc
    18301 acagaataat gaaagtgtgt aaagaagggt aaaaaaaacc ggatgcgagg catccggttg
    18361 aaataggggt aaacagacat tcagaaatga atgacggtaa taaataaagt taatgatgat
    18421 agcgggagat attctagttg cgagtgaagg ttttgttttg acattcagtg ctgtcaaata
    18481 cttaagaata agttattgat tttaaccttg aattattatt gcttgatgtt aggtgcttat
    18541 ttcgccattc cgcaataatc ttaaaaagtt cccttgcatt tacattttga aacatctata
    18601 gcgataaatg aaacatctta aaagttttag tatcatattc gtgttggatt attctgcatt
    18661 tttggggaga atggacttgc cgactgatta atgagggtta atcagtatgc agtggcataa
    18721 aaaagcaaat aaaggcatat aacagagggt taataacatg aaagttaaag tactgtccct
    18781 cctggtccca gctctgctgg tagcaggcgc agcaaacgct gctgaagttt acaacaaaga
    18841 cggcaacaaa ttagatctgt acggtaaagt agacggcctg cactatttct ctgacaacaa
    18901 gtcagaagac ggcgaccaga cctatgtacg tcttggtttc aaaggcgtaa ctcaggttac
    18961 tgaccagctg accggttacg gccagtggga atatcagatc cagggcaata cctctgaaga
    19021 caacaaagaa aactcctgga cccgtgtggc attcgcaggt ctgaaattcc aggatgtggg
    19081 ttctttcgac tacggtcgta actacggcgt tgtttacgac gtaacttctt ggaccgacgt
    19141 actgccagaa ttcggtggcg acacctacgg ttctgacaac ttcatgcagc agcgtggtaa
    19201 cggcttcgcg acctaccgta acactgactt cttcggtctg gttgacggcc tgaactttgc
    19261 tgttcagtac cagggtaaaa acggtagcgt aagcggcgaa ggcatgacca acaacggtcg
    19321 tggtgctctg cgtcagaacg gcgacggcgt tggcggatct atcacttatg attacgaagg
    19381 cttcggtatc ggtggtgcga tctccagctc caaacgtact gatgatcaga acagcccgct
    19441 gtacatcggt aacggcgacc gtgctgaaac ctacaccggt ggtctgaaat acgacgctaa
    19501 caacatctac ctggctgctc agtacaccca gacctataac gcaactcgcg taggttccct
    19561 gggttgggcg aacaaagcac agaactttga agctgttgct cagtaccagt tcgacttcgg
    19621 tctgcgtccg tctgtagcat acctgcagtc taaaggtaaa aacctgggta ccatcgctgg
    19681 tcgtaactac gacgacgaag atatcctgaa atatgttgat gttggtgcta cctactactt
    19741 caacaaaaac atgtccacct acgttgacta caaaatcaac ctgctggacg acaaccagtt
    19801 cactcgtgac gctggcatca acactgataa catcgtagct ctgggtctgg tttaccagtt
    19861 ctaatctcga ttgatatcga acaaagggcc tgcgggccct tttttcattg ttttcagcgt
    19921 acaaactcat ttttttggtg tactcttgcg accgttcgca tgaggataat cacgtatgga
    19981 aataagcttt acccgcgtgg cactgctggc tgccgcgctc ttctttgttg gttgcgatca
    20041 aaaaccacaa cccgccaaaa cccacgctac tgaagttacc gttctagaag gcaaaaccat
    20101 gggtaccttc tggcgtgcca gcatcccggg cattgatgcc aaacgcagtg ccgaacttaa
    20161 agaaaagatt cagacccagc tggacgccga cgatcagctg ctttcgacct ataaaaaaga
    20221 ttccgcgctg atgcgcttta acgattcgca aagtttatcg ccgtggccgg taagtgaagc
    20281 gatggccgat atcgtcacca cctcgctgcg cattggcacg aagaccgatg gcgcgatgga
    20341 tataaccgtc gggccgctgg tgaatctgtg gggctttggc ccggaacaac agtcggttca
    20401 aattccgagc caggaacaga tcgatgcgat gaaagccaaa accggcttac agcacctgac
    20461 ggtcattaat cagtcgcatc agcaatatct gcaaaaagac ctgccggatt taaatgtcga
    20521 tctctccacc gtcggtgaag gttatgcggc ggatcacctg gcacgcttga tggagcagga
    20581 agggatttcc cgctatctgg tgtcggtggg cggcgcgctg aacagccgtg gtatgaacgg
    20641 tgaaggcctg ccgtggcggg tagcgattca aaaaccaacc gataaagaaa acgcggttca
    20701 ggctgtggtg gatatcaacg gtcatgggat tagcacctct ggcagctacc gtaactatta
    20761 cgaactggac ggcaaacgtc tttcccatgt tatcgatccg caaaccgggc gtcccatcga
    20821 acacaatctg gtatccgtga cggtgattgc tccgacggcg ctggaagccg atgcctggga
    20881 tactggcttg atggtactcg ggccggagaa agccaaagag gttgttcgcc gggaagggct
    20941 ggcggtttat atgatcacca aagaaggcga tagctttaaa acctggatgt caccacagtt
    21001 taaaagcttc cttatcagcg aaaaaaatta aagcgcaaga ttgttggttt ttgcgtgatg
    21061 gtgaccgggc agactaaagg ctatccttaa ccagggagct gattatgaaa aacgccacat
    21121 gcttaactga cgatcaacgc tggcaatctg tcttagcccg cgacccgaat gccgacggcg
    21181 aattcgtttt cgccgtgcgc accacgggta tcttttgccg tccgtcttgc cgcgccagac
    21241 atgccttgcg ggaaaatgtc tccttctacg caaatgccag cgaggcactc gccgccggat
    21301 ttcgcccctg caaacgttgt cagccagaca aagccaatcc ccggcaacat cggttggata
    21361 aaatcaccca cgcgtgtcga ctgctggaac aggaaacgcc tgtaacgctg gaagccttag
    21421 ccgaccaggt ggcgatgagt ccatttcatc tacatcggtt gtttaaagcg actaccggaa
    21481 tgacgcctaa agcctggcaa caggcctggc gcgctcgccg tttgcgcgaa tcgctggcga
    21541 aaggggagag cgtgacgacg tctattctta acgccggatt ccccgacagc agcagttact
    21601 accgcaaagc cgatgaaacg ctgggcatga cggctaaaca attccgtcac ggtggcgaaa
    21661 atctggcggt gcgttacgcg ctggctgatt gtgagctggg tcgttgcctg gtggcagaaa
    21721 gcgagcgggg gatttgcgcg atattgctgg gcgatgatga cgcgacatta atcagcgagt
    21781 tgcagcagat gtttcccgct gccgacaacg cgcctgccga tctgacgttt cagcaacatg
    21841 tgcgtgaagt gatcgccagc ctcaatcaac gcgatacgcc gctgacgtta ccgctggaca
    21901 ttcgcggcac tgcttttcag caacaagtct ggcaggcact gcgcacgata ccttgtggtg
    21961 aaaccgtcag ttatcagcaa ctggctaacg ccatcggcaa accgaaagcg gtacgggccg
    22021 ttgccagcgc ctgtgccgcc aacaagctgg ctatcgtaat accctgtcat cgagtggtcc
    22081 gtggtgatgg cacactttcc ggttaccgct ggggcgtgtc gcgtaaagcg caactgctgc
    22141 gccgcgaagc tgaaaatgag gagaggtaat gttggatctg tttgccgatg ctgaaccgtg
    22201 gcaagagcca ctggcggctg gtgcggtaat tttacggcgt tttgctttta acgctgcgga
    22261 gcaactgatc cgcgatatta atgacgttgc cagccagtcg ccgtttcgcc agatggtcac
    22321 ccccggggga tataccatgt cggtggcgat gaccaactgt gggcatctgg gctggacgac
    22381 ccatcggcaa ggttatctct attcgcccat tgatccgcaa acaaataaac cgtggcccgc
    22441 catgccacaa agctttcatg atttatgtca acgtgcggct acggcggcgg gctatccaga
    22501 tttccagcca gatgcttgtc ttatcaaccg ctacgctcct ggcgcgaaac tgtcgctgca
    22561 tcaggataaa gacgaaccgg atctgcgcgc gccgattgtt tctgtttctc ttggcttacc
    22621 cgcgattttt caatttggtg gcctgaaacg taatgatccg ctcaaacgtt tgttgttgga
    22681 acatggcgat gtggtggtat ggggcggtga atcgcggctg ttttatcacg gcattcaacc
    22741 gctgaaagcg gggtttcatc cactcaccgg cgactgccgt tacaacctga cattccgtca
    22801 ggcaggtaaa aaagaataaa aataagaatt attattgctg tgcgcgcgaa gatgtttaaa
    22861 ctgcgggctg taattcattg tccgggtttt ctgcatggaa cttcttgtac ttgtctggcg
    22921 gcagtatcgc tggccattta tcagtgtgat ggcgctaagc ctcgccagtg cggctttagg
    22981 cattggctta attgctttta tcaatcagcg ccttatcgaa acggcggata ccagtctgct
    23041 ggtgttgccg gagtttctgg gattattgct gctgttgatg gcaatcactc tcggatcgca
    23101 actggcgctc accactttgg ggcatcactt cgtttaccga ctgcgtagtg aatttatcaa
    23161 gcggattctc gatacccacg tcgagcgcat tgaacaactc ggtagcgcct cgttgctggc
    23221 gggattaacc agcgatgtgc gcaatatcac cattgctttt gtgcgtctgc cggaactggt
    23281 gcaggggatc attctcacta tcggttcagc ggcgtatctg tggatgctgt cgggcaaaat
    23341 gttgctggta acggctatct ggatggcggt caccatctgg ggcggttttg tgctggtggc
    23401 gcgggtgtac aaacatatgg cgaccctgcg tgaaaccgaa gataagctgt acacggattt
    23461 tcaaaccgta ctcgaagggc gcaaagagct gactctgaac cgtgaacgcg ccgagtatgt
    23521 gtttaacaac ctctacattc ctgatgcgca agagtatcgc catcatatta tccgcgcaga
    23581 caccttccat cttagtgccg tgaactggtc aaacatcatg atgctgggcg ctatcggcct
    23641 ggtgttctgg atggcgaaca gcctcggttg ggctgatacc aacgttgctg cgacctattc
    23701 gttgacgctg ttattcctgc gtacgccgct gctttcggcg gttggcgcat tgccgacgct
    23761 gctgacggcg caggtggcgt ttaacaagct gaacaaattc gcgctcgcgc ctttcaaagc
    23821 agagtttccg cgcccgcagg cgtttcccaa ctggcaaacg ctggagctgc gtaatgtgac
    23881 gtttagctat caggataacg cgttttccgt tgggccgatt aacctcacca ttaaacgtgg
    23941 cgagctgctg tttctgattg gtggcaacgg tagtggaaaa tcgacgctgg cgatgttgct
    24001 gacgggcttg taccagccac aaagcggcga aatcttgctg gatggcaaac ctgtcagcgg
    24061 cgaacaaccg gaagattatc gcaaactgtt ttcggcagtg tttaccgatg tctggctgtt
    24121 tgatcaactg ctggggccgg aggggcaacc cgctaacccg caactggttg agaagtggct
    24181 ggcgcagctg aaaatggctc ataagcttga gttaagcaac gggcgtattg ttaacctgaa
    24241 gttatcaaaa gggcagaaaa aacgcgtggc gctgttgctg gcgctggcag aagaacgcga
    24301 tattatcctg ctggatgaat gggcggcgga tcaggatcca cacttccgtc gtgagtttta
    24361 tcaggtgttg ctgccgctga tgcaggagat gggtaaaact attttcgcca tcagtcatga
    24421 tgatcattac tttatccacg ccgaccgcct gctggaaatg cgcaatgggc aacttagcga
    24481 gctgacgggc gaagagcgcg atgccgcttc gcgtgatgcc gttgcccgga cggcataata
    24541 ttacgacgct gacctggcgg actgctgccg ccaggtcaat attctaaaaa tcccttcatt
    24601 tctaataacc ctataattaa tcaacgaaat tataatgttt ctaaaattag aatataattt
    24661 ataaacatta tttaaatgtt gttactttag tgttaccgtt gatgccgcgc aaatctcacc
    24721 tgcaataaag cgactaaaag taaggcatta acaagatgaa aaaagtgact gccatgctct
    24781 tctcgatggc cgtggggctt aatgccgttt cgatggcggc aaaagcgaaa gcgtccgagg
    24841 agcaggaaac tgatgtactg ttgattggcg gcggcattat gagcgccacg ttggggacct
    24901 atttacgcga gctggagcct gaatggtcga tgaccatggt ggagcgcctg gagggtgtcg
    24961 cgcaggagag ttcgaacggc tggaacaacg ccggaaccgg gcattctgca ctgatggaac
    25021 tgaactacac cccgcaaaac gccgatggca gtatcagtat tgaaaaagca gtcgccatta
    25081 acgaagcatt tcagatttcc cgccagttct gggcgcacca ggtcgagcgc ggcgtgctgc
    25141 gtactccgcg ttcatttatc aataccgttc cgcatatgag ctttgtctgg ggcgaggata
    25201 acgttaattt cctgcgcgcc cgttacgccg cgttgcaaca aagctcgctg tttcgcggta
    25261 tgcgttactc tgaagatcac gcgcagatca aagagtgggc accgttagtg atggaagggc
    25321 gcgatccgca acagaaagtg gcagccacgc gtacggaaat tggtaccgat gtgaactacg
    25381 gcgagatcac ccgccagtta attgcttcct tgcagaagaa atctaacttc tcgctgcaac
    25441 tcagcagcga agtccgcgcc ctaaagcgta atgacgataa cacctggacc gttaccgttg
    25501 ccgatctgaa aaatggcact gcacagaaca ttcgtgccaa atttgtcttt atcggcgcgg
    25561 gcggtgcggc gctgaagctg ttacaggaat cggggattcc ggaagcgaaa gactacgccg
    25621 gtttcccggt gggcggacag ttccttgttt cggaaaaccc ggacgtggtt aatcaccatc
    25681 tggcgaaggt ttacggtaaa gcatccgttg tcgcaccacc gatgtcggtt ccgcatatcg
    25741 atacccgcgt tctggacggt aaacgcgtag tgctgtttgg gccatttgcc accttctcaa
    25801 ccaaattcct caaaaacggt tcattgtggg atctgatgag ttccaccacc acctctaacg
    25861 tgatgccgat gatgcacgtc gggctggata atttcgatct ggtgaaatac ttggtgagtc
    25921 aggtgatgtt gagtgaagag gatcgttttg aagcgttgaa agagtactat ccgcaggcga
    25981 aaaaagagga ctggcgtttg tggcaagcgg ggcagcgtgt gcagattatc aagcgtgatg
    26041 ccgataaagg tggtgtactg cgtctgggta ctgaagtggt cagtgaccag caagggacca
    26101 ttgccgcgct gctgggggca tcgccagggg cgtcaaccgc tgcgccgatt atgttggatc
    26161 tgctggaaaa agtatttggc gatcgcgttt ccagcccgca atggcaggct acgttgaaag
    26221 cgatcgttcc atcttatgga cgcaagctga acggtgatgt agcggcaaca gaacgcgagt
    26281 tgcagtacac cagcgaagtg ctgggcctga aatacgacaa gccgcaggca gcagatagta
    26341 cgccgaaacc gcagttgaaa ccgcaacccg ttcaaaaaga agtggcggat attgcgttgt
    26401 aatgatgtgc cacatccggc atggtatgcc ggatgtggcg taaccgcctt atccgaccta
    26461 cggggtggag cacttcacag cggtgtaggc ctgataagac gcgtcagcgt cgcatcaggc
    26521 aaccggctcg ggcgttagat gtcggatgcg gcgtaaccgc cttatccaac ctacggggtg
    26581 gagcacttca cagcggtgta ggcctgataa gacgcgccag cgtcgcatca ggcaaccggc
    26641 ttgggcgtta gatgtcggat gcggcgtgac cgccttatcc gacctacggg gtgacgcact
    26701 tcacagcagt ttagcgaact accgcgttgt caattttctc ttccgccttc cagacgcggt
    26761 acttcacatc tacattgtct ggcgtataca ccacgatcgg cagcttgctg ttgtaacgca
    26821 gcattccagc atcgcccaga tacgcggtga caaatttctt ctctttcttg ccatccgggc
    26881 aggccatcat cgtggaaacc ggcgagctaa ctttatcaaa gacataataa tcatagcccc
    26941 agccttccag cgttttgttt tccagcttcc cgccgagacg atgcaaattg caatcgactt
    27001 ccagcgtctg accggttaac agttctactt tcagggtaga ttcatcttct tgcggggtta
    27061 actgaatcac ctgacgcttc atcccttttt cagcttgtgg ataaggcgcg attttttcca
    27121 gtggctggac gctttctgcc gcccaggcgg aagtggtggc gaaagcggca aacaatactg
    27181 caggtagaat ggtcttcatt tttttgcttt ccttcttcat ttataggttt tcaccgatga
    27241 tgtcgctgaa ggttaacata ttcatcagat ttattaattt gctttatgaa taacttaaag
    27301 aacattcaga caattttacc tgctctttta gtgttaaatt ctgatgaggg agcaaaccag
    27361 cagaaatatc tctggtgaat taatcgctat ataaatatgt ttataaccat ttgaaatgtg
    27421 agcaaaagcc cgtttttgcc acgcttcccg ccactctttt gatcctgcta caggttttac
    27481 cccgatcggg gtatgcatct ttgacacatc ctttaatatc ttagcggcta taaaaatggc
    27541 ttattaatta tgcggtttat ttggtcgctc tcaattttca gagcgcgtta atgatggaag
    27601 gtcaatgtga agattgatgc atcccgtcgg ggcatactca ctggtcgctg gcgcaaagcc
    27661 agtaacggta tccgtccgcc ctggtccggt gatgaatctc attttctgac ccattgcacc
    27721 cgttgcgacg cctgtattaa tgcttgtgaa aacaacattc tgcaacgcgg cgcgggcggc
    27781 tatccgagcg ttaatttcaa aaataacgag tgcagcttct gttacgcctg cgcgcaggct
    27841 tgccccgaat cgctattttc tccgcgccac accagggcct gggatttgca gttcaccatt
    27901 ggggacgcgt gcctggcgta tcagtcagtt gaatgccgcc gctgtcagga tagctgtgag
    27961 ccaatggcaa ttatctttcg cccgacgctc tccgggatct accagccgca acttaatagc
    28021 caactttgca acggatgtgg agcatgtgca gccagctgtc cggtatcagc cataactgcg
    28081 gagtatctcc atgcacacta actggcaagt ttgcagcctg gtcgtgcagg ccaaaagcga
    28141 acgaatttca gacatcagca cccaactgaa cgcctttccc ggctgtgaag ttgctgtcag
    28201 cgacgcgccg agcggtcagt tgattgtggt ggtggaagca gaagacagcg aaacgctgat
    28261 ccaaaccatt gagtcagtac gcaacgtaga gggcgtgctg gcggtgtcgc tggtttatca
    28321 ccagcaggaa gagcaaggtg aggaaacacc atgaaactca gtcgtcgtag ctttatgaaa
    28381 gctaacgccg ttgcggccgc tgcggcggct gccggtctca gcgtgccggg cgttgcccgc
    28441 gccgttgttg gtcagcagga agccattaaa tgggataaag cgccgtgccg tttctgcggt
    28501 actggttgcg gcgttctggt cggaacgcag caggggcgtg tggtggcctg tcagggcgac
    28561 ccggacgcac cggttaaccg tggcctgaac tgcattaagg gctatttcct gcccaagatc
    28621 atgtacggta aagaccgttt gacgcagccg ctgctgcgta tgaaaaacgg taaatatgac
    28681 aaagaaggcg aatttacccc aatcacctgg gatcaggcct tcgatgtgat ggaagagaaa
    28741 ttcaaaaccg ccctgaaaga aaaagggccg gaatcgatcg gtatgttcgg ttctggtcag
    28801 tggactatct gggaaggtta tgccgcgtcc aagctgttta aagcgggctt ccgttcgaac
    28861 aacatcgacc cgaacgcgcg tcactgtatg gcgtcggcag tagttggctt tatgcgtacc
    28921 tttggtatgg atgagccgat gggctgctat gacgacatcg agcaggctga cgcgtttgtg
    28981 ctgtggggcg caaacatggc ggagatgcac ccgatcctct ggtcgcgcat cactaaccgt
    29041 cgtctctcta atcagaacgt caccgtggcg gtgctttcta cctaccagca tcgtagcttc
    29101 gagctggcgg ataacggcat catctttacg ccacagtctg acctggtgat cctgaactac
    29161 atcgccaact atatcattca aaacaatgcg ataaatcagg acttcttcag caaacacgtt
    29221 aacctgcgca aaggggcgac ggacatcggc tacggtttac gtccgaccca tccgctggaa
    29281 aaagcagcga agaatccagg ttctgacgcc tccgaaccga tgagctttga agattacaaa
    29341 gccttcgttg ccgagtatac gctggaaaaa accgctgaaa tgaccggcgt gccgaaagac
    29401 cagttagaac aactggcgca gctgtatgcc gatccgaaca agaaagtcat ctcctactgg
    29461 acgatgggct tcaaccagca tactcgtggc gtgtgggcta acaacctggt ctacaacctg
    29521 cacctgctga ccggcaaaat ttcccagccg ggttgcggtc cgttctccct gaccgggcaa
    29581 ccttctgcgt gtggtactgc gcgtgaagtg ggcacctttg ctcaccgtct gcctgcggac
    29641 atggtggtga ccaacgagaa acaccgtgat atctgcgaga agaagtggaa tatcccgagc
    29701 ggcaccattc cggcgaaaat cggtctgcac gcggtggcac aagaccgtgc gctgaaagac
    29761 ggcaaactga atgtttactg gaccatgtgt accaacaaca tgcaggccgg gccgaacatc
    29821 aatgaagagc gtatgccggg ctggcgcgat ccgcgcaact tcatcatcgt ctccgatccg
    29881 tatccgacag tcagtgcgct ggccgccgac ttgatcctgc cgaccgcaat gtgggtagag
    29941 aaagagggtg cttacggtaa cgccgaacgc cgtactcagt tctggcgtca gcaggtacag
    30001 gcaccgggcg aagcgaaatc ggatctctgg cagttagtcc agttctcccg ccgcttcaaa
    30061 actgaagaag tgtggccgga agagctgcta gcgaagaaac cggaactgcg tggcaaaacg
    30121 ctgtacgaag tgctttatgc cacaccggaa gtgagcaaat tcccggtatc cgaactggcg
    30181 gaagatcagc tgaacgatga atcccgcgag ctgggcttct atctgcaaaa agggctgttc
    30241 gaagagtacg catggtttgg tcgcggtcac ggtcacgatc tcgcgccgtt cgatgactac
    30301 cacaaagcgc gcggtctgcg ctggccggtg gtgaacggta aagaaacgca gtggcgttac
    30361 agcgaaggta acgacccgta cgtgaaagcg ggcgaaggct ataagttcta cggtaaaccg
    30421 gatggtaaag cggtgatctt cgcgctgccg ttcgaaccgg cggcggaagc accggatgaa
    30481 gagtacgacc tgtggctctc taccggacgc gttctggagc actggcacac cggcagtatg
    30541 actcgccgtg tgccggaact gcaccgcgcc ttcccggaag cggtcttgtt tattcacccg
    30601 ctggatgcga aagcgcgcga tctgcgccgt ggcgacaaag tgaaagtggt ttctcgccgt
    30661 ggcgaagtga tctcgattgt tgaaacgcgc ggtcgtaacc gtccgccaca gggcctggtg
    30721 tacatgccgt tcttcgacgc cgcacagctg gttaacaaac taacgctgga cgcgaccgat
    30781 ccgctctcga aagagacgga cttcaagaag tgcgcagtca aactggagaa ggtgtaagcc
    30841 attatgtccc ggtcagcgaa acctcaaaat ggtcgccgcc gctttctgcg cgatgttgtt
    30901 cgcacagcag gcgggctggc tgccgtgggt gtggcgctgg ggttacaaca gcaaaccgca
    30961 cgcgcatctg gcgtgcggtt gcgcccgccc ggagccataa acgagaacgc ctttgccagt
    31021 gcctgtgtgc gttgcggtca gtgtgttcag gcttgccctt acgacacctt aaaactggcg
    31081 acgctggctt ctggcctgtc ggcgggcacg ccatattttg tcgcacggga tattccttgc
    31141 gaaatgtgtg aggacattcc gtgcgccaaa gtgtgcccga gtggtgcgct ggatcgtgag
    31201 atcgaatcga tcgacgacgc gcggatgggg ctggcggtgc tggtggacca ggaaaactgt
    31261 ctcaactttc aggggctgcg ctgcgatgtt tgttatcgcg aatgcccgaa aattgatgag
    31321 gccatcaccc tggagctgga gcgcaacacg cgtaccggta agcacgcccg ctttctgccg
    31381 acggttcaca gcgacgcctg taccggctgc ggtaagtgcg aaaaagtgtg cgtgctggaa
    31441 caaccggcaa tcaaggtgtt accgctgtca ctggcgaaag gggagttagg tcaccattac
    31501 cgcttcggct ggctggaggg gaacaatggc aaatcgtaaa cgtgacgccg ggcgcgaggc
    31561 gctggtgaaa aaaggctggt ggcgcagtca ccgttggctg gtgttacgtc gcctttgtca
    31621 gttcttcgtg ctggggatgt ttttgagcgg tccgtggttt ggtgtgtgga tcttacacgg
    31681 caactacagc tgtagcctgt tattcgacac cgttccgctg accgatccgc tgatgacgct
    31741 gcaaagtctt gccagtggtc atctgcctgc cacggtggcg ttgaccggtg cggtgataat
    31801 cactgtgctc tacgccctgg caggaaagcg attattttgc agctgggtct gcccgctgaa
    31861 cccgattacc gacttagcga actggttacg caggcgcttt gacctcaatc agtctgcgac
    31921 gatccctcgc cacattcgct acgtgctgtt ggtggtcatt ctgctgggat cagccttaac
    31981 cggcacgctc atctgggaat ggatcaaccc cgtttccctg atggggcgta gcctggtaat
    32041 gggcttcggc agcggcgcgc tgcttattct cgcgctgttt ttatttgatc tactggtcgt
    32101 tgaacacggc tggtgcgggc acatttgtcc ggttggcgcg ctgtatggcg tgctgggtag
    32161 caaaggtgtg attaccgttg cggccagcga tcggcagaaa tgtaaccgct gtatggattg
    32221 ttttcatgtt tgcccggaac cgcatgtgct acgtgccccg gtgctggatg aacaaagccc
    32281 ggtgcaggtc accagccgcg attgcatgac gtgcggtcgc tgcgtggatg tctgttctga
    32341 ggatgtattt acaataacta cacgatggag ttcgggagcg aaatcatgaa aagccatgac
    32401 ctgaagaaag cgctgtgtca atggacggcg atgctggccc tggtggtaag cggcgcggtt
    32461 tgggcggcta atggcgtcga ttttagccag tcgccggaag tatccgggac gcaggaaggg
    32521 gccattcgga tgccaaaaga gcaggatcgg atgccgctga actatgtgaa tcagccgcca
    32581 atgatcccgc atagcgttga aggttatcag gtaacgacca ataccaaccg ctgcttgcag
    32641 tgccacggtg tcgaaagcta tcgcaccact ggcgcgccgc gtatcagtcc tactcacttt
    32701 atggacagcg acggcaaagt gggcgcggaa gtggctccgc gtcgttattt ctgtctgcaa
    32761 tgtcacgtac cgcaggccga taccgcgcca atcgtgggga atacctttac cccatcaaaa
    32821 ggttacggga aataagaggt tattatggga aattctgacc gtaagcctgg tctgattaag
    32881 cgcctgtgga aatggtggcg tacccccagc cgtctggcgc tgggaacgct gctgttgatc
    32941 ggttttgtcg gcggcatcgt cttctggggc ggctttaaca ccggaatgga aaaagccaat
    33001 accgaagagt tctgcattag ctgccacgaa atgcgtaaca cggtgtatca ggaatacatg
    33061 gattccgtgc actacaacaa ccgtagcggc gtccgtgcga cctgtccgga ttgtcacgtt
    33121 ccgcacgagt ttgtgccgaa gatgatacgc aagctcaaag caagtaaaga gctgtatggt
    33181 aaaatttttg gcgttattga cacgccgcag aaatttgaag ctcatcgtct gacaatggca
    33241 cagaatgagt ggcggcgcat gaaggacaat aactcgcagg agtgccgtaa ctgtcacaac
    33301 ttcgagtata tggatacaac cgcccagaaa tcggttgccg cgaagatgca tgaccaggcg
    33361 gtgaaagatg ggcaaacctg tattgattgc cataaaggga tagcgcacaa gctgcccgat
    33421 atgcgtgaag tcgagccagg tttttaacag ggttattgcg tgggtatgct tgaagccaga
    33481 gagttacttt gtgagcggga tgaacgaacc ttatttagtg gcttgtcatt tacgctgaac
    33541 gcaggagagt gggtacaaat caccggtagc aacggcgcgg ggaagacaac gcttctccgt
    33601 ttgctgacgg ggttgtctcg ccctgacgca ggcgaggttc tctggcaagg gcagcccttg
    33661 catcaggtac gcgacagcta ccatcaaaac ctgttatgga taggccatca gccggggatc
    33721 aaaacccggc tgacggcgtt agaaaatctg cacttttatc atcgcgatgg cgataccgca
    33781 caatgtctgg aagccctggc gcaggccggg cttgccggat tcgacgatat tccggtaaat
    33841 caactttctg ccgggcaaca acgccgcgtc gctttagcgc gtctgtggct gacccgtgcc
    33901 acgttatgga tcctcgacga gccttttacc gcgattgacg ttaacggcgt cgatcgtctg
    33961 acccagcgta tggcgcagca tacggagcag ggggggattg tgattctgac tacccaccag
    34021 ccgctcaacg ttgctgaaag taaaattcgc cgcatttcac tgacgcaaac gagggccgca
    34081 tgatgttctg gcgcattttc cgtcttgagc tgcgtgtagc gtttcgccat agcgccgaaa
    34141 tcgccaaccc gctgtggttc ttcctgattg taattaccct ttttccgctc agtatcggtc
    34201 cggagccgca actgctggcg cgtattgcac cgggcattat ctgggttgct gcgctgcttt
    34261 catccttgct ggcgctggaa cgactgttcc gtgacgattt gcaggacggc agtcttgaac
    34321 aattgatgtt gttgccgtta cccttgcccg ccgttgtgct ggcgaaggtg atggcgcact
    34381 ggatggtaac cggtctgccg ttactcatcc tttcgccact ggtagcaatg ctactgggaa
    34441 tggatgttta tggctggcaa gtgatggcgc tgacgctgct gctgggaacg cctacgcttg
    34501 gctttctcgg tgcaccgggt gtggcgttga cagtgggact taagcgcggt ggtgtgctgc
    34561 tcagcatact ggtgttaccg ctgactatcc cattactcat ctttgccacc gccgcgatgg
    34621 acgcggcttc catgcatttg cccgttgacg ggtatctggc aattttaggc gcgttgctgg
    34681 caggcaccgc gacattaagt ccttttgcga cggcggcagc gttacgaatc agcattcaat
    34741 aacgctggat tactgatttt tgttcgagtc tggtatcgaa actatgtgga aaacactgca
    34801 tcaactggcg atcccaccac ggctgtatca aatctgtggc tggtttatac cgtggctggc
    34861 aattgccagt gtggtcgtgc ttaccgtcgg ctggatctgg ggattcggct ttgctccggc
    34921 tgattatcag cagggaaata gctaccgcat tatctacctg catgtgcctg cggcgatctg
    34981 gtcgatgggc atttatgcat caatggcagt ggcagcgttt attggccttg tctggcagat
    35041 gaaaatggcc aacctggcgg tggcggcgat ggcccccatt ggtgccgtgt ttacctttat
    35101 tgccctggtt accggctctg catggggaaa accgatgtgg ggcacctggt gggtatggga
    35161 tgcacgtctg acttctgaac tggtgctgct gtttttgtat gtgggtgtga ttgccctgtg
    35221 gcacgccttc gacgaccgcc gtctggcggg ccgtgcggca ggtatcctgg tgctgattgg
    35281 cgtggtgaat ctgccgatta ttcattactc cgtggagtgg tggaacaccc tgcatcaggg
    35341 atcaacgcgg atgcagcaaa gtatcgatcc ggcgatgcgt tcgccgctgc gctggtcgat
    35401 ttttggcttc ctgctcctgt ctgccacgct gacgctgatg cggatgcgta atttgatttt
    35461 gctgatggaa aaacgccgtc cgtgggtgag tgaactgata ctgaaaagag gccgtaaatg
    35521 acccctgcat ttgcttcctg gaatgaattt ttcgcaatgg gcggttacgc cttttttgtc
    35581 tggctggcgg tggtgatgac cgttattccg ctggtggttt tggtcgtgca ctcggtgatg
    35641 caacatcgcg caattctgcg tggtgtggcg caacagcggg cgcgtgaggc gcgtttacgt
    35701 gctgcgcaac agcaggaggc tgcatgaata ttcgccgtaa aaaccgcttg tggattgcct
    35761 gtgccgtgtt ggcagggctg gcgctgacta tcggtctggt gctatatgcg ctgcgctcga
    35821 atatcgatct cttttatacg ccgggggaaa ttctctacgg caagcgtgaa actcagcaaa
    35881 tgccggaagt cggtcagcgt ctgcgcgttg gcgggatggt gatgctgggt agtgtgcagc
    35941 gcgatcccaa ttcgctgaaa gtgaccttca ctatttacga tgctgaaggc tcagtggatg
    36001 tctcttacga aggcattttg ccggatctgt tccgtgaagg gcagggcgtt gtggtgcagg
    36061 gcgagctgga aaaaggcaat catatcctcg cgaaagaagt gctggcgaaa cacgacgaaa
    36121 actacacgcc gccagaagtt gagaaagcga tggaagctaa ccaccgtcgc ccggcgagtg
    36181 tttataagga cccagcatca tgatgccgga aattggaaac gggctgctgt gtctggcgtt
    36241 agggattgcg ctgctgctgt ccgtgtatcc gctatggggc gtggcgcgcg gagatgcgcg
    36301 catgatcgcg tcttcccgct tgtttgcctg gctgctgttt atgtctgtgg ctggcgcatt
    36361 tctggtgctg gtcaatgctt tcgtggtcaa cgactttacc gtcacctatg ttgccagcta
    36421 ctccaatacc cagcttccgg tgtggtatcg cgtggcggct acctggggcg cgcatgaagg
    36481 ctcgctactg ctgtgggtgc tgctgatgag cggctggacc tttgcggtgg cgatttttag
    36541 tcagcgtatt ccgctggata ttgtggcccg cgtactggcg ataatgggga tggtcagcgt
    36601 cggctttttg ctgttcattc tctttacctc taacccattc tcacgcacgc tgccgaactt
    36661 cccgattgaa gggcgcgatc ttaacccgct gttacaggac ccggggctga tcttccatcc
    36721 gcctctgctt tatatggggt acgtgggttt ctcggtggcg tttgcttttg ccattgcttc
    36781 tttgctgagc gggcgtctgg acagcactta tgcgcgtttt actcgcccgt ggacgctggc
    36841 ggcgtggatt ttcctgacgc tcggcattgt gctcggttcc gcatgggcct attacgaact
    36901 cggctggggg ggctggtggt tctgggatcc ggtagaaaac gcctcgttta tgccgtggct
    36961 ggtggggact gcgctgatgc actcactggc ggtcactgaa caacgcgcca gcttcaaagc
    37021 gtggacatta ctgctggcaa tcagtgcctt ctcgttgtgt ctgttgggga ctttcctcgt
    37081 gcgttccggc gtgctggtat cggtacacgc gtttgcgtct gatccggcgc gcggtatgtt
    37141 tatcctcgcc tttatggtgc tggtgattgg aggttcgctg ctgctgtttg ccgcgcgtgg
    37201 acacaaagtt cgctcacgcg taaacaatgc gctgtggtcg cgggaatctc tgctgttagc
    37261 gaacaacgtt ttgctggtcg ccgcgatgct ggtggtgttg ctggggacgc tgctgccgct
    37321 ggtgcacaag caactgggac tgggcagtat ttcgattggc gaaccgttct tcaacaccat
    37381 gtttacctgg ctgatggtgc cgtttgcgct actgctgggt gtcggtcctc tggtgcgctg
    37441 ggggcgcgat cgcccacgta aaatccgcaa tttattgatt atcgccttca tctcaacgct
    37501 ggtgctgtct ctgctgttgc cgtggctgtt tgagagcaaa gttgtggcga tgacggtgct
    37561 cggcctggca atggcctgct ggattgcggt gctggcaatt gcggaagctg cgctgcgtat
    37621 ttcacgcggc acgaaaacca ccttcagtta ttgggggatg gtggctgctc acctggggct
    37681 ggcagtgaca attgttggca ttgcttttag ccagaactat agcgttgagc gtgatgtgcg
    37741 catgaagtcc ggcgatagcg tcgatattca tgaatatcgc ttcaccttcc gtgatgtcaa
    37801 agaggtgact ggcccgaact ggcgtggcgg tgtggcgact atcggcgtaa cgcgagaggg
    37861 taaaccggaa acagtgctgt atgcggaaaa acgttattac aacactgccg ggtcgatgat
    37921 gaccgaagcg gcgattgacg gtggcatcac gcgtgacctg tacgccgcgc tcggtgaaga
    37981 gctggaaaac ggcgcgtggg ctgtgcgtct ttactacaaa ccatttgttc gctggatttg
    38041 ggcgggcggg ctgatgatgg cgttgggcgg actgctgtgt ctgtttgatc ctcgctatcg
    38101 taagcgcgtg agtccgcaaa aaactgcgcc ggaggctgta tgaagcgcaa agtattgtta
    38161 attccgttga ttatcttcct ggcgattgcc gcggcgctgc tgtggcagct ggcgcgtaat
    38221 gccgaagggg atgatccgac caatctggaa tcggcgctca ttggcaagcc agtgccgaag
    38281 tttcgcctcg aatcactgga caatccgggg cagttttatc aggcggatgt gctgactcag
    38341 ggcaaaccag tgctgcttaa cgtctgggcg acctggtgtc cgacctgccg tgcggaacat
    38401 caatatctga atcagctttc tgcgcagggc atccgcgtgg tcggcatgaa ctataaagac
    38461 gatcgccaga aggcaatcag ctggctgaaa gagctgggca atccttacgc gctaagcctg
    38521 tttgatggcg atggcatgtt agggctggat ctcggtgtct atggcgcgcc agaaacgttc
    38581 cttattgacg gcaacggcat cattcgctat cgccatgcgg gcgatctcaa tcctcgcgtc
    38641 tgggaagaag agatcaagcc gctgtgggag aaatacagta aggaggccgc acaatgaggt
    38701 ttttattggg catgctgatg ctgatgatct ccggctcagc gctggcgacc atcgacgtgt
    38761 tgcagtttaa agatgaagca caggaacaac agttccgtca gctcactgaa gaactgcgct
    38821 gcccgaaatg ccagaacaac agcattgccg attccaactc gatgattgcc accgacctgc
    38881 gccagaaagt gtatgaactg atgcaggaag gtaaaagtaa aaaagagatt gtcgattata
    38941 tggtggcgcg ttacggcaac ttcgtcactt acgatccgcc gttaacgccg ctgaccgtgc
    39001 tgctgtgggt gctgccagta gtagctattg gcattggcgg ttgggtcata tacgcccgct
    39061 cgcggcgtcg ggtacgcgtg gtgccggaag cgtttcctga acaaagcgtg ccggaaggta
    39121 agcgtgccgg atatgtcgct tatttgccgg gcattgtggt ggcgttaatt gtggctggcg
    39181 tcagctacta ccagaccgga aattatcaac aggtgaaaat ctggcagcag gccacggcac
    39241 aggctccggc gttgctggac agggcgctgg atccgaaagc tgatccgctc aacgaagaag
    39301 agatgtcgcg ccttgcgctg gggatgcgta ctcaactgca aaaaaatccg ggagatatag
    39361 acggctggat tatgttgggc cgcgttggca tggcgctggg taacgccagt atcgccaccg
    39421 atgcatacgc tactgcgtat cgcctcgatc cgaaaaacag tgatgccgca ctgggttatg
    39481 ctgaagcgtt gacacgttca tctgatccca acgacaaccg cctcggcggc gaactgctac
    39541 gtcagctggt gagaacggac catagcaata tccgtgtgct aagcatgtat gcgtttaatg
    39601 cctttgagca gcagcgattt ggcgaagccg ttgccgcgtg ggagatgatg ttgaaactct
    39661 tacctgccaa cgatactcgc cgtgcggtga ttgaacgtag tatcgcgcag gcgatgcaac
    39721 atttgtcgcc gcaggagagt aaataaaaag aggcccgatg gtgatgccat cgggctattt
    39781 ttattgtgcc ccgcgttgtt gcaggaacag aatggtggcc gccacgcgtg agcggacatt
    39841 gagtttacgc agcagatttc gaatatgaac ttttactgtc tgctcggaaa tattcaacac
    39901 agaggcaatc tgtttatttg acagcccctg tgccagctcg tgcagaacat ccagctcgcg
    39961 ctccgtcagc acgctgaagg gatcttcttc cgcgccaaac atttcacgtt cacgtaagta
    40021 ctgattgacg cgttcgctaa agactttgct gcctttcgct ccggcacgaa tcgcttccag
    40081 caacacttcc gggtcgctgt ctttcagcag ataaccgtct gcgcctgcgt ctatcagcgc
    40141 aaagacatcg ctggaggcat cggatacggt caggataata atttgcgcgg taacgccatc
    40201 cctgcgcaag gcattgaggg tatccaggcc actcatacct ttcatattga gatccagcaa
    40261 gatcacgtcg atatccagtc tattcgccag atcgatcgcg ctcgcgccgt cgcccgcttc
    40321 ggcgaccact tcaaagccag gatcaagctc cagtaactga cgaacaccgc gtcgcataag
    40381 tggatgatca tccacaatca tcacctgaaa aggcgttgct tcaggcatag tagtctcctg
    40441 aggttttatt agaatcatta tgtttgagcg tagcgttggt aacgctacca ggcgcgtgaa
    40501 tgttacccca ttttatgtat ggacatgtag tgaaaactac aaccttgccg ttcgctttgg
    40561 catcattttc attataaaga cggaattttg catcagcttc tcatcaagaa aaacaactaa
    40621 aaaatcaatc acaatacttg tgcatttctt taaacagttt ttaaaaactg actccgggta
    40681 tggagctatg ggtattttct gtacccaatg cttttaacag caattaattt cataggatga
    40741 aagctcaatg catcaatctg gttctgtttc tctttgtcgt tccgcaatat ctgttctggt
    40801 ggctacagcg ttatattcac ccatagcatt ggcatcaaca gttgagtatg gtgagacagt
    40861 tgatggtgtt ggcctggaaa aagatatcca gctggtttat gggaccgcca ataatacgaa
    40921 aatcaatcct ggcggagaac agcatataaa agaatttggt gtaagtagta atactgaaat
    40981 taacggcggg tatcagtaca ttgaaatgaa tggcactgca gaatactcag tattaaatga
    41041 tggttatcaa attgttcaaa tgggtggcgc ggtaaaccag actacgctca ataatggagt
    41101 gctacaggtt tatgg
//
LOCUS       DAKISA010000031        40288 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.031, whole genome shotgun sequence.
ACCESSION   DAKISA010000031 DAKISA010000000
VERSION     DAKISA010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40288
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.031"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(29..1990)
                     /gene="topB"
                     /locus_tag="OVW45_001661"
     CDS             complement(29..1990)
                     /gene="topB"
                     /locus_tag="OVW45_001661"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCV5526137.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERALDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLLQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDVVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
     gene            complement(1995..3038)
                     /gene="selD"
                     /locus_tag="OVW45_001662"
     CDS             complement(1995..3038)
                     /gene="selD"
                     /locus_tag="OVW45_001662"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCV5526138.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(3155..3706)
                     /locus_tag="OVW45_001663"
     CDS             complement(3155..3706)
                     /locus_tag="OVW45_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCV5526139.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            3867..5723
                     /gene="sppA"
                     /locus_tag="OVW45_001664"
     CDS             3867..5723
                     /gene="sppA"
                     /locus_tag="OVW45_001664"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCV5526140.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            5890..6906
                     /gene="ansA"
                     /locus_tag="OVW45_001665"
     CDS             5890..6906
                     /gene="ansA"
                     /locus_tag="OVW45_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCV5526141.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            6917..7558
                     /gene="pncA"
                     /locus_tag="OVW45_001666"
     CDS             6917..7558
                     /gene="pncA"
                     /locus_tag="OVW45_001666"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCV5526142.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHSLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(7719..7991)
                     /locus_tag="OVW45_001667"
     CDS             complement(7719..7991)
                     /locus_tag="OVW45_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCV5526143.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKANCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(8033..8446)
                     /gene="msrB"
                     /locus_tag="OVW45_001668"
     CDS             complement(8033..8446)
                     /gene="msrB"
                     /locus_tag="OVW45_001668"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCV5526144.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            8788..9783
                     /gene="gapA"
                     /locus_tag="OVW45_001669"
     CDS             8788..9783
                     /gene="gapA"
                     /locus_tag="OVW45_001669"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCV5526145.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            9867..10751
                     /locus_tag="OVW45_001670"
     CDS             9867..10751
                     /locus_tag="OVW45_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCV5526146.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLTVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHNEDADGVALTFELTQSEETKKFCPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(10802..11656)
                     /gene="yeaE"
                     /locus_tag="OVW45_001671"
     CDS             complement(10802..11656)
                     /gene="yeaE"
                     /locus_tag="OVW45_001671"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HCV5526147.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(11746..12492)
                     /gene="mipA"
                     /locus_tag="OVW45_001672"
     CDS             complement(11746..12492)
                     /gene="mipA"
                     /locus_tag="OVW45_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCV5526148.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            12928..14862
                     /gene="yeaG"
                     /locus_tag="OVW45_001673"
     CDS             12928..14862
                     /gene="yeaG"
                     /locus_tag="OVW45_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCV5526149.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMKGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            14975..16258
                     /locus_tag="OVW45_001674"
     CDS             14975..16258
                     /locus_tag="OVW45_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HCV5526150.1"
                     /translation="MTGFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            16405..17880
                     /locus_tag="OVW45_001675"
     CDS             16405..17880
                     /locus_tag="OVW45_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCV5526151.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQKLQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            18061..19551
                     /locus_tag="OVW45_001676"
     CDS             18061..19551
                     /locus_tag="OVW45_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCV5526152.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            19594..20097
                     /gene="yeaK"
                     /locus_tag="OVW45_001677"
     CDS             19594..20097
                     /gene="yeaK"
                     /locus_tag="OVW45_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCV5526153.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(20098..20202)
                     /locus_tag="OVW45_001678"
     CDS             complement(20098..20202)
                     /locus_tag="OVW45_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5526154.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            20372..20818
                     /locus_tag="OVW45_001679"
     CDS             20372..20818
                     /locus_tag="OVW45_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCV5526155.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(20775..21596)
                     /gene="nimR"
                     /locus_tag="OVW45_001680"
     CDS             complement(20775..21596)
                     /gene="nimR"
                     /locus_tag="OVW45_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HCV5526156.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            21693..22840
                     /gene="nimT"
                     /locus_tag="OVW45_001681"
                     /pseudo
     CDS             21693..22840
                     /gene="nimT"
                     /locus_tag="OVW45_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            22895..23242
                     /locus_tag="OVW45_001682"
     CDS             22895..23242
                     /locus_tag="OVW45_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCV5526157.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(23264..23518)
                     /gene="yoaF"
                     /locus_tag="OVW45_001683"
     CDS             complement(23264..23518)
                     /gene="yoaF"
                     /locus_tag="OVW45_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HCV5526158.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            23701..24726
                     /gene="dgcP"
                     /locus_tag="OVW45_001684"
     CDS             23701..24726
                     /gene="dgcP"
                     /locus_tag="OVW45_001684"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HCV5526159.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGISTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNPRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHC"
     gene            24759..24857
                     /locus_tag="OVW45_001685"
     CDS             24759..24857
                     /locus_tag="OVW45_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HCV5526160.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            24860..24934
                     /gene="yoaJ"
                     /locus_tag="OVW45_001686"
     CDS             24860..24934
                     /gene="yoaJ"
                     /locus_tag="OVW45_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HCV5526161.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(24993..25241)
                     /locus_tag="OVW45_001687"
     CDS             complement(24993..25241)
                     /locus_tag="OVW45_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCV5526162.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(25389..25571)
                     /locus_tag="OVW45_001688"
     CDS             complement(25389..25571)
                     /locus_tag="OVW45_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HCV5526163.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(25575..25934)
                     /gene="yeaR"
                     /locus_tag="OVW45_001689"
     CDS             complement(25575..25934)
                     /gene="yeaR"
                     /locus_tag="OVW45_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HCV5526164.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFEVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(26107..26745)
                     /gene="leuE"
                     /locus_tag="OVW45_001690"
     CDS             complement(26107..26745)
                     /gene="leuE"
                     /locus_tag="OVW45_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HCV5526165.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(26872..27794)
                     /gene="dmlR"
                     /locus_tag="OVW45_001691"
                     /pseudo
     CDS             complement(26872..27794)
                     /gene="dmlR"
                     /locus_tag="OVW45_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
     gene            27897..28982
                     /gene="dmlA"
                     /locus_tag="OVW45_001692"
     CDS             27897..28982
                     /gene="dmlA"
                     /locus_tag="OVW45_001692"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HCV5526166.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            29233..30843
                     /gene="yeaV"
                     /locus_tag="OVW45_001693"
     CDS             29233..30843
                     /gene="yeaV"
                     /locus_tag="OVW45_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HCV5526167.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANTIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISTWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            30875..31999
                     /gene="yeaW"
                     /locus_tag="OVW45_001694"
     CDS             30875..31999
                     /gene="yeaW"
                     /locus_tag="OVW45_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HCV5526168.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            32055..33020
                     /gene="yeaX"
                     /locus_tag="OVW45_001695"
     CDS             32055..33020
                     /gene="yeaX"
                     /locus_tag="OVW45_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCV5526169.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(33074..34189)
                     /gene="rnd"
                     /locus_tag="OVW45_001696"
     CDS             complement(33074..34189)
                     /gene="rnd"
                     /locus_tag="OVW45_001696"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCV5526170.1"
                     /translation="MITTDDALASLCEAVRVFPAIAQDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(34271..35956)
                     /gene="fadD"
                     /locus_tag="OVW45_001697"
     CDS             complement(34271..35956)
                     /gene="fadD"
                     /locus_tag="OVW45_001697"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCV5526171.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLIDMFEQSVVRYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCCRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(36161..36742)
                     /gene="yeaY"
                     /locus_tag="OVW45_001698"
     CDS             complement(36161..36742)
                     /gene="yeaY"
                     /locus_tag="OVW45_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCV5526172.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MNAPDLYVGQEARFGGKVVVVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGTVDGKVNNAPYKFMVMQVTGYKRWHITQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVETIVTE"
     gene            complement(36782..37477)
                     /gene="tsaB"
                     /locus_tag="OVW45_001699"
     CDS             complement(36782..37477)
                     /gene="tsaB"
                     /locus_tag="OVW45_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCV5526173.1"
                     /translation="MRILAIDTATEVCSVALWNDGNVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLRKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(37535..39445)
                     /locus_tag="OVW45_001700"
     CDS             complement(37535..39445)
                     /locus_tag="OVW45_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCV5526174.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGCAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDLLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            39577..39921
                     /locus_tag="OVW45_001701"
     CDS             39577..39921
                     /locus_tag="OVW45_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCV5526175.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         9948 a        10284 c         9763 g        10293 t
ORIGIN      
        1 actcgaatag cacgaaagat tcactcgctt acgctatcgc cccgcttccg acttcatctg
       61 ctggcggact ttttttcgca ctacgtttac gcggtgcagc ctttttctta tcagcactgc
      121 caccactgcc cggagccaca atgccgcgaa actgccgcac cggcgtacgt ttggcttgat
      181 caataagctg atatagcgtc cccaccagcg gctgcataaa gtcctgatag cgacactgct
      241 tttcgctgat ttgcgtcagc accgattccc agtgcgcggt catgtccggt cgcgtcgcca
      301 tttctggcag cgaatggaat aacgcttttc cggcgtcggt agagtggata tagcgccctt
      361 ttttggtcag aaaaccacgc ttgaacagca gttcaataat cccggcacgg gttgcttctg
      421 tccccagacc atcggtcgca cgaaggatct ttttcagatc tttgtcctgc acaaagcgcg
      481 cgatcccggt catcgccgaa agcagtgttg catcggtaaa atggcgcggc ggctgggttt
      541 gccgctctac cacttcacct ttttcacaca gcaactcatc gcctttcgcc accacaggca
      601 atggcgtacc gtcgttttct tcatcgcgct ctttgctgcc taacagcgtg cgccagcctg
      661 cttcagcaag aaaacgcgct ttagcgacaa atttgccttt ggcaatgtcc agttcgataa
      721 cacacttgcg gaacaccaca tccgggcaga actgcatcaa gtactgacgg gcaatcaggt
      781 tatagacctt cgcttcgttc tccgtcaggt tgatggctga actccgtgcg gtcgggatga
      841 tggcgtggtg tgcatcgacc tttttgtcat cccaacagcg gttgcgtata tctggatcta
      901 ccactggctg cagcaacaga tctggtgcat gaacgctgat ggcattcatc accgcgtggc
      961 gtccggcaaa atgttcttct ggcaaatagc gacaatcgga acgcggataa gtgattagct
     1021 tgtgcgtttc gtacagcttc tggcagatat caagcacgtt ctgcgcgctc agaccaaagc
     1081 gttttgccgc ttcaatctgc aacgccgaaa gcgaaaaagg cagcggcgcg gattctgatt
     1141 cccgtttatc gttatagctg gtgacaattg ctggttgacc actaatgcgg ttaaccacat
     1201 gctccgccag tggacgatgt aacaagcgcc cttcttcatc ctggtacggt tcacacgctt
     1261 cgctcggttg ccagatagcg gtaaaccgct catcggcagg tgtcacgata tgtgctttga
     1321 cttcaaagaa gtctttcgcc acgaagtttt caatctcttc atcgcggcgc accaccagcc
     1381 cgagcacggg tgtctgcacg cgtcccacgg aaagtacgcc ctgataaccg gcattgcgac
     1441 cgagaatggt atacgcacgg gtcatattga tgccgtacag ccagtcggca cgcgctcgcg
     1501 ccagcgcaga aacgcacaat ggcacaaact cactgttgga ccgaagacgg tcgagcgccc
     1561 gctcaaccgc ctgcgggttc aggtcgttta tcaagcaacg ctgcacctgc tggcgctttt
     1621 ccggtgccag ttgcagatag tccagcactt catccaccag cagttgccct tcacgatccg
     1681 ggtccccggc gtgaacgatt tcgctggctt catgcaggaa gcgtttgatg acgttaagtt
     1741 gtttggtcac ggagggtcgg ggctgtaatt gccacttttc cgggacaatc ggcaaatccg
     1801 caagattcca gcgcgcatag cggctgtcgt aggcgtctgg ctgcgcctgc tcaagcaggt
     1861 gaccgataca ccaggtcacc acctgaccat taccgcactc gataaagcca tcgcctttcc
     1921 ggtgcggttt gggcaggaca tcagcaatgg cgcgcgccag actcggtttt tcggcaataa
     1981 acaaccgcat tgaattaacg aatctcaacc atggcacgac cgccgcgcgc tggcaccagt
     2041 tcgccaattg ccgtcagttc aatgccaaac tcggcggctg tagctttgac ctcattttct
     2101 gcttccggca tgaccgccag cagcaaaccg ccagaagttt gcggatcgca cagcaaatcg
     2161 cgcacttcac gcggcatttc acccatcaga tgaccgtagc tggcaaagtt acgttcggtg
     2221 ccgccaggca ctgcgcccaa cttaatgtac tcttcaaccc ccggcaattt tgggatcgcg
     2281 tcatagtcaa cgcgcgcctg cacacctgcg ccctgacaca tttcgctcaa atggcccagc
     2341 agaccaaaac ccgtaacgtc ggtcatcgct tttacgcctt cgatgttggc aaaggacgcg
     2401 cctgcgatgt tcatccggca catcacttcc gtcgctagtc cctgatgttc tggtttcagc
     2461 agtgattttt tctcagccgt ggtaagaacg ccgatcccca gcggtttcgt caggaacagt
     2521 ttgcatccgg cttgtgcggt actgtttttc ttcacccgct cggtcggtac gatccccgtt
     2581 accgccagac caaaaatcgg ctccggcgca tcgatggagt gaccgccagc cagcgcaata
     2641 cccgcctgac ggcatgcata gcgtccacct tcggttactt cgcgggcaat ttccggggaa
     2701 agtttgttaa tcggccagcc gaggatcgca atcgccataa tcggtttgcc ccccatcgcg
     2761 aagatatcgc tgatggcgtt agtcgccgca atgcggccaa aatcgaaagg attatcaacg
     2821 atcggcataa agaagtcggt ggtactgata acgctggtgc cattgcccag atcgtacacc
     2881 gccgcatcgt cgcgggtctc attacccaca agcaaattcg gatcaacaaa cttcgcctgc
     2941 tcactgtgca ggatggtttc caacactttt ggggaaattt tacagccgca accagctccg
     3001 tggctgtatt gggtcaaacg aatcgagttc tcgctcatgg acatctcctg tcaatgcaat
     3061 ccgggtatgg taaccctcat tccgtgaagt gataagtgag agtgtctgaa ttcctgcgcc
     3121 tttgctcaca atccagacag tttcgcgaca attatcagaa ataagttaca aacggcgtcg
     3181 ggtccgggac gttaatcgac gtagatgctt tcaactgcgg cgtgccgagg tagagaaaac
     3241 cgacaatttt atcctgctca cggcaaccga atgcttcacg cactaccgga ctttcagtta
     3301 atgcgccact gcgccagatg ccgccaaatc cctgggcaac ggctgccatt tgcatcgcca
     3361 tgaccgcgca tccggcagac atttcctgtt cccatcgcgg gactttatga ttttcttcgc
     3421 atttcgccac caccgtgatg atgagcggtg cgcggaacgg cgcattacgg gctttgtcga
     3481 tagctttgtc atcactaccg gcagcaatcg ccccctgttc cagtacggcg ctgaaacgct
     3541 cgcgcccttc cccttcaatc acaaaaaaat gccacggttg catggactta tggtccggcg
     3601 cacgcatacc cgcacgcagg atgttttgca gttgttcacc cgttggcgcg ggttcagcca
     3661 ggcgggaggc gctacggcga ttgatcaata gttcgagtgc atccatttga ttaactcctg
     3721 tcgtgatatt tattcacaaa attaacacga gagtggattt tgttacagca cagtccgcaa
     3781 ttcctgctga caagtgccgg ttgggtcatt acgataacca catctattgc gcctgtgaca
     3841 ggtgtgacct taagttggga gaatacatgc gaaccctttg gcgatttatt gccggatttt
     3901 ttaaatggac gtggcgtctg ctgaatttcg tccgtgaaat ggtacttaac ctgttcttta
     3961 ttttcctcgt actggttggt gtggggatct ggatgcaggt cagtggtggt gattcgaaag
     4021 aaacggccag tcgtggcgca ctgctgctgg acatttctgg tgtgatcgtc gataaacccg
     4081 acagttctca gcggtttagt aaattaagcc gccagctgct tggtgccagt tccgatcgtc
     4141 tgcaggaaaa ctcactgttt gatatcgtca acaccattcg ccaggcgaag gacgaccgca
     4201 atatcaccgg tatcgtgatg gatctgaaaa acttcgcagg cggcgaccag ccgtctatgc
     4261 agtacatcgg caaagccctg aaagagtttc gtgacagcgg gaaaccggtt tatgccgttg
     4321 gcgagaacta cagccagggg caatattatc tcgccagttt cgccaataaa atttggctgt
     4381 caccgcaagg cgtggtggat ttgcacggtt ttgctaccaa cggtctgtac tacaaatcgt
     4441 tgctggataa gctgaaagtt tccacccatg tgttccgcgt gggtacgtat aaatctgccg
     4501 ttgaaccgtt tattcgtgat gacatgtcac cggcagcccg cgaagctgac agccgctgga
     4561 ttggtgagct gtggcaaaac tatctgaata ctgttgccgc taaccggcag atccctgctc
     4621 agcaggtatt ccctggcgcg caagggttgc ttgagggttt aaccaaaacc ggtggcgata
     4681 ccgcgaaata tgcactggaa aacaagctgg tcgatgcact ggcatcgagt gccgaaatcg
     4741 aaaaagctct aaccaaagag ttcggctgga gtaagactga taaaaattat cgcgccatca
     4801 gttattacga ttacgcattg aaaacgccgg cagataccgg tgacagcatc ggtgtcgtct
     4861 tcgctaatgg cgcaattatg gatggcgagg aaactcaggg gaatgttggc ggtgatacca
     4921 ctgcggcaca aatccgcgac gctcgccttg acccgaaagt gaaagcgatt gtcctgcgtg
     4981 ttaatagccc aggcggcagc gttaccgcgt ctgaagtgat tcgcgctgaa ctggcagcag
     5041 cccgggcagc gggtaagcct gtggttgtat cgatgggcgg catggcggca tctggtggtt
     5101 actggatttc cacgccagct aattacattg tggctaaccc cagcaccctg accggttcta
     5161 tcggtatctt cggcgtgatc accaccgtag aaaatagtct ggattcgatt ggtgttcata
     5221 ctgatggtgt ctcaacttca ccgctggcgg atgtttctat caccagggca ctgccgccgg
     5281 aagcgcagca gatgatgcaa ttaagcattg agaatggcta taaacgcttt atcacgctgg
     5341 ttgctgatgc gcgtcattcg acgccggagc agattgataa aatcgcccag ggccacgtct
     5401 ggaccggtca ggatgcaaaa gctaacgggc tggtcgatag tctcggggat ttcgatgatg
     5461 cggtcgccaa agcagcagag ctggcaaaag tgaaacagtg gcatctggaa tactacgttg
     5521 atgaaccgac cttcttcgac aaagtgatgg acaacatgtc tggttctgtc cgggcaatgt
     5581 tgccagatgc gttccaggcc atgttacctg caccgcttgc ctcggtagcc tctaccgtta
     5641 aaagtgaaag cgacaagctg gccgcgttta acgacccaca aaaccgttat gcgttttgcc
     5701 tgacctgcgc caacgtgcgt taagtcttgt actgagtggc cgacagatcg tcggccacat
     5761 tattttttac gtcgacgaat cctcttcccg ctgtctcgcc ccatatttcc ttatccgcat
     5821 agtatcaggt gcgctcctcc tgcctcacgt atatactttt gctctttcga tatcattcat
     5881 atcaatatca tgcaaaagaa atcaatttac gtcgcctaca cgggcgggac catcgggatg
     5941 cagcgttccg agcagggtta tattccggtg tcaggtcatc tacaacgcca actggcgctg
     6001 atgccggaat tccatcgccc ggagatgcca gatttcacca ttcatgaata tacgccgctg
     6061 atggattctt ctgatatgac gccagaagac tggcagcata ttgctgaaga tattaaagcg
     6121 cactatgacg actatgatgg ttttgtcatc ctgcacggca ccgacacgat ggcgtatact
     6181 gcctctgcgc tgtcgttcat gctcgagaat ctcggtaaac cggtcattgt gacagggtca
     6241 caaatcccgc tggctgagtt acgctctgac ggacaaatta atctgctgaa tgcgttgtac
     6301 gttgcggcga attatccgat caacgaagta acgctctttt tcaataaccg attgtatcgc
     6361 ggcaaccgca ctaccaaagc ccatgccgat ggttttgatg cgtttgcctc tccaaacctt
     6421 cctccgttac tggaagctgg tatccatatt cgtcgtttga atacgccacc cgccccgcac
     6481 ggtgaagggg aattaatcgt tcatccaatc acgccacaac caattggcgt agtgacgatt
     6541 tatccaggga tttctgctga cgtcgtgcgc aattttctgc gccaaccggt gaaagcattg
     6601 attctgcgct cctatggcgt gggtaatgcg ccacaaaaca aagccttcct gcaggaatta
     6661 caagaagcca gcgatcgcgg tattgtggtg gtcaacctga cacaatgtat gtccggtaaa
     6721 gtgaatatgg gtggttatgc caccggtaac gccctcgccc atgccggcgt tattggcggt
     6781 gcagatatga ctgtagaagc cacgctaacc aaactgcatt acctgctgag ccaggaactg
     6841 gatactgaaa ccattcgcaa ggccatgagc caaaacctgc gcggcgaact gacgccggat
     6901 gattaaggag actgtaatgc cccctcgcgc cctgttactg gtcgatttac aaaatgattt
     6961 ctgtgctggt ggcgcgctcg ccgtgccgga aggtgacagt acggtggatg tcgctaaccg
     7021 cctgattgac tggtgccagt cgcgcggtga agcggttatc gccagtcagg actggcaccc
     7081 ggcgaatcac ggcagttttg ccagtcagca cggtgtagag ccttatacgc caggccaact
     7141 cgacggtttg ccacaaacct tctggccaga tcactgtgtg cagaacagtg aaggcgcaca
     7201 attacattcg ttactgaacc aaaaagcgat cgcagcggtg ttccataaag gcgaaaatcc
     7261 tttagttgac agttacagtg ccttttttga taacggccgt cggcagaaaa cctcgctcga
     7321 tgactggtta cgcgatcatg aaatcgatga attgatcgtt atgggcctgg ctactgacta
     7381 ttgcgtgaag tttaccgtgc tggacgcgtt acagttaggt tataaggtaa acgtgattac
     7441 cgatggttgt cgtggcgtga atatccagcc ccaggacagt gcgcacgcgt ttatggagat
     7501 gtcagcagct ggggcaacgc tatatacgct ggcagactgg gaagagacac aggggtaata
     7561 ttagttgaat tcatctctgg cctacatttc ctcaatgaca ttgtaggcca gataatactt
     7621 gtcagtgttg catttaacct acttgcagct aaaacacgac gtcctaacaa gtatatttgc
     7681 cctcccttaa taacaggctt aaatactaga taactcattc acttaaacat agcaataggc
     7741 tttgctgaga taccaaaatc ttcttttaac tgctgtttgc tcttcatgac catctgacca
     7801 tttgtgtcaa ttgtcatatg ctgtgcttcg gtattatggc gagcttgcca cagcatcacc
     7861 agttgcaggc agttcgcttt ttgttcctcg gtcaacgcaa cgccatcggg ccatttcccc
     7921 agttcaacgg cggtcgacaa acgctggtat acctcaggcg tcatgctgtt gataatgtca
     7981 tcaagattca taatttttcc gctcctgtgg aataagttgc tgaatcgttt tttcaaccgt
     8041 tgatttcttc gccgttttcg ccatcagtga agcgtaaaga agcagagtta acacaataac
     8101 gttcgcccgt tggctgcggc ccgtcaggga agacatgccc cagatgggca tcacagttac
     8161 cgcaacgaat ttctatgcgc tgcattccat gtgacaagtc tttgatataa cgaatggatt
     8221 cttcactcac cggttcgtag aaactgggcc aaccacagcc ggaatcatac ttggtttggg
     8281 aatgaaacag cggggcatcg cagatcaaac agtgatatac gccgtcacgc ttgttatgca
     8341 gtaaacgacc cgtaaatggc ggctctgtcc catgattctg cgtcacgtaa aactgcatct
     8401 cggacaaatt ttttttcagt tcttctgccg aaggtttatt agccatttgc tcacatctca
     8461 ctttaatcgt gctcacatta cgtgactgat tctaacaaaa cattaacacc aactggcaaa
     8521 attttgtcct aaacttgatc tcgacgaaat ggctgcacct aaatcgtgat gaaaatcaca
     8581 tttttatcgt aattgccctt taaaattcgg ggcgccgacc ccatgtggtt tcaagcccaa
     8641 aggaagagtg aggcgagtca gtcgcgtaat gcttaggcac aggattgatt tgtcgcaatg
     8701 attgacacga ttccgcttga cgctgcgtaa ggtttttgta attttacagg caacctttta
     8761 ttcactaaca aatagctggt ggaatatatg actatcaaag taggtatcaa cggttttggc
     8821 cgtatcggtc gcattgtttt ccgtgctgct cagaaacgtt ctgacatcga gatcgttgca
     8881 atcaacgacc tgttagacgc tgattacatg gcatacatgc tgaaatatga ctccactcac
     8941 ggccgtttcg acggtaccgt tgaagtgaaa gacggtcatc tgatcgttaa cggtaaaaaa
     9001 atccgtgtta ccgctgaacg tgatccggct aacctgaaat gggacgaagt tggtgttgac
     9061 gttgtcgctg aagcaactgg tctgttcctg actgacgaaa ctgctcgtaa acacatcacc
     9121 gctggtgcga agaaagtggt tatgactggt ccgtctaaag acaacactcc gatgttcgtt
     9181 aaaggcgcta acttcgacaa atatgctggc caggacatcg tttccaacgc ttcctgcacc
     9241 accaactgcc tggctccgct ggctaaagtt atcaacgata acttcggcat catcgaaggt
     9301 ctgatgacca ccgttcacgc tactaccgct actcagaaaa ccgttgatgg cccgtctcac
     9361 aaagactggc gcggcggccg cggcgcttcc cagaacatca tcccgtcctc taccggtgct
     9421 gctaaagctg taggtaaagt actgccagaa ctgaatggca aactgactgg tatggcgttc
     9481 cgcgttccga ccccgaacgt atccgtagtt gacctgaccg ttcgtctgga aaaagctgca
     9541 acttacgagc agatcaaagc tgccgttaaa gccgctgctg aaggcgaaat gaaaggcgtt
     9601 ctgggctaca ccgaagatga cgtagtatct accgatttca acggcgaagt ttgcacttcc
     9661 gtgttcgatg ctaaagctgg tatcgctctg aacgacaact tcgtgaaact ggtatcctgg
     9721 tacgacaacg aaaccggtta ctccaacaaa gttctggacc tgatcgctca catctccaaa
     9781 taagttgaga tgaaactgtg atctaaaaag agcgacttcg gtcgctcttt tttttacctg
     9841 ataaaatgaa gttaaaggac tgcgtcatga ttaagaaaat ttttgccctt ccggtcatcg
     9901 aacaaatctc ccctgtcctc tcccgtcgta aactggatga actggacctc actgtggtcg
     9961 atcatcccca ggtaaaagcc tcttttgcat tacagggcgc acaccttctc tcgtggaaac
    10021 ctgcgggtga agaagaagtt ctgtggttga gcaacaacac accgttcaaa aatggcgtcg
    10081 ctattcgcgg tggcgtaccg gtttgctggc cgtggtttgg tccggcggca caacaaggtc
    10141 tgcctgcgca cggttttgcc cgcaacctgc cgtggacgct gaaatcacat aatgaagatg
    10201 ctgatggcgt agcgctgact tttgaattga cgcaaagcga agagacgaaa aaattctgtc
    10261 cgcacgactt tacgctgtta gcgcatttcc gcgtgggtaa aacctgtgaa atcgatcttg
    10321 aatcacatgg cgaatttgaa accacctctg ccctgcatac ctactttaac gtgggtgata
    10381 tcgctaaggt aagcgtcagt gggctgggcg atcgcttcat tgataaagtg aatgacgcga
    10441 aagaagatgt actgaccgac ggtattcaga ccttccctga ccgtaccgat cgcgtgtatc
    10501 tgaatccgca agattgcagc gtgattaatg atgaagcgct gaatcgtatt atcgccgtag
    10561 gccaccagca tcatctgaac gttgtaggct ggaacccggg accggcgctt tcaattagca
    10621 tgggcgatat gccggatgat ggctacaaaa catttgtttg tgtagaaacg gcttacgctt
    10681 cagaaacgca aaaagtcacc aaagagaaac ctgcacatct ggcgcaatcc attcgcgttg
    10741 cgaaacgtta atttgcgtta atgttgtgtg ccgggtgcga tgcatccggc acgtatcaac
    10801 atcacaccat atccagcgca gtttttcctt ttggtgccgg atacgcttta tccagcatgg
    10861 ttaattccgc tgacgaaagt tcgacctcaa gcacagccgc attctgttga acatgggcaa
    10921 tcgtggccgc ttttggaatc gccatcacac cctgatggct gattacccac gccaacaata
    10981 cttgtgccga gctgatattg tgagcatgtg caatttcgtt gactaccgcg ttttttaaca
    11041 gtccattgcg caaccgcccg gcctgggcta atggactgta agccatcacc ggcatctgct
    11101 gttgctggca ccaggggagt agatcgtact caatccctcg tgaaccgaga tggtaaagca
    11161 cctgattagt ggcacactga tttccccccg gcagctgcca gagttcctgc atatcagcat
    11221 aatcaaggtt agaaacgccc cagcggcgga tttttccctg ggcgatcaat ttttccatcg
    11281 ctgcaacagt ctcttcaaaa gcgaaactgc cagaccagtg taataagtag agatcgagat
    11341 aatcagtatt gagacggcgt aaactggctt cgcatgcatt tatcgctttt tgcccgccag
    11401 cattccacgg atagacttta gagacgagaa agacgttatc acgcagaccg gttaatgctt
    11461 ccccaaccac cttttcggca ccgccatcgg catacatttc ggcggtatca atgagggtta
    11521 aaccgagctc aatgcccgcg cgtagtgcag caacttctgt tttgcgctga ctggcatctt
    11581 cgcccatata ccatgttccc tgccctatgg ctggcagtga gacatcgcca ctaaattgaa
    11641 tcattttttg ttgcattgtt tcctcccagg tattgcacca ccgtaatgca aaacagggcg
    11701 tgacgccctg tttttatgca caaaatgccc tggaaagatg cattatcaga atttgtaggt
    11761 gatcccggta gaaatcaggc cagtccagga tttatccacc atcgggctgt cagtaacttc
    11821 atcagacaga cgggtatagc gcgcggtacc gtaaacactc cagtcgccga ggaagttgta
    11881 gctggcgctc agctccaggt aagggctcca gctgtcgttc gggttatagc cacgcagacc
    11941 gctgcgagcg gactctttgc gcgatacgcc ataatagtat tcgttctggt tttcgctgtt
    12001 ccactgcaca ccgatacccg gggtcactgt caggccgccg ttggtgtaac gatacaacca
    12061 ggccatatcc cagacaatgc cgttgctgtt atccagggta tcgccagcca gggtggtacg
    12121 caggtaaccg tactgggtaa agtgagcata agacagacca gccatcatgg tgctcttacg
    12181 gtcatccagg tgacgcattt ggtgatcgcc actgtctttc gctttgaagt aaagcggcga
    12241 ccaataagcg gtaattgaaa gtttgtccgt tgcgtcattc cacaggtagt aaccaccacc
    12301 taagccacgg aaccagaagt tatcgccttc atagttgatt accggtactg ggtaaacatc
    12361 ggtatcgtaa tctttatatg ggtgttcaac gacaccgacg cctgcgccca gggaaaattt
    12421 accttcagcg tgcgctacgc ctgcagacgt tgcgataagc actccaagtg ccagaagttt
    12481 gagtttggtc acaattaatc attccttaaa caaatgttta gcgggcgaca aagtttaccc
    12541 gtcaatacat cgaaacccaa cctttttacg ttttcatttt ttaaagtaac tgtttaattt
    12601 tcctgacgcg gatgacaccg cgcttacagc caaatgaatt tagcgttact ggcgagcctt
    12661 gtctttacat taattatgca aaatttatgg atgagttgtt gatatgccat tgaaattaag
    12721 aaagccgtgc aggcaagttt tccatttgcc atctacgctt aattttgaag gtgtatcacc
    12781 gggcacgttg ttctcatcgt cgataaaatg gcatgagagt tgctgtgttt tagcaagaga
    12841 cgtcgttcag tttacctctt ccgggagcct ctactattca tatgaacggc tcttaacctg
    12901 tgctaaaaaa cgaaaggacg gcataccatg aatatattcg atcactatcg ccagcgatat
    12961 gaagctgcca aggacgaaga gttcacactg caggagtttc ttaccacttg tcggcaagat
    13021 cgcagtgctt atgccaacgc ggctgagcgg ctattgatgg ctatcggtga gcctgtcatg
    13081 gtcgatacag cccaggaacc cagactttct cgactctttt ctaaccgggt cattgcacgt
    13141 tatccggcat ttgaagagtt ttacggcatg gaagacgcga ttgaacagat tgtctcttat
    13201 ctgaaacacg cggctcaggg gctggaagag aagaaacaaa tcctgtatct gctggggcct
    13261 gtgggtgggg gtaaatcatc gcttgctgag cgactgaaat cattaatgca gctcgtaccg
    13321 atttatgtat tgagcgccaa cggtgagcgt agcccggtca acgatcatcc gttctgtctt
    13381 ttcaatccgc aggaagatgc gcagattctg gaaaaagagt atggcattcc tcgccgctat
    13441 ctcggcacca tcatgtcgcc gtgggcggca aaacgcctgc atgaatttgg tggcgatatc
    13501 actaagttcc gggtagtgaa ggtctggccg tcaattctgc aacaaattgc tatcgccaaa
    13561 acagaacccg gtgatgagaa caaccaggac atctccgcgc tggtagggaa agtcgatatt
    13621 cgtaaactcg aacactacgc gcagaatgac ccggacgcct acggctattc cggtgcgctg
    13681 tgccgcgcca atcaggggat catggaattt gtcgagatgt ttaaagcacc gattaaagtg
    13741 ctgcatccgc tgttaaccgc tactcaggaa ggtaactaca acgggacgga aggtatctcc
    13801 gccctgccgt tcaacgggat tattctcgcc cactcgaacg aatccgaatg ggtcactttc
    13861 cgtaataaca aaaacaacga agccttcctc gaccgtgttt acatcgtgaa ggtgccgtat
    13921 tgcttacgca tttccgaaga gatcaaaatc tacgagaaat tgcttaatca cagtgaattg
    13981 actcacgccc catgcgcccc tggcacgctg gaaacactgt cacgtttttc cattctttcg
    14041 cgcctgaaag agccagaaaa ctccagcatt tattcaaaga tgcgggttta tgatggcgaa
    14101 agtctgaaag acaccgatcc caaagccaag tcgtatcagg aatatcgtga ctacgccggt
    14161 gtcgatgaag ggatgaaagg tctgtcgacg cgttttgcgt ttaagatcct ctcccgcgtg
    14221 ttcaacttcg atcatgtaga agtggcggca aacccggtcc atctgttcta cgtcctggaa
    14281 cagcagatcg agcgcgagca gttcccgcaa gagcaggcag aacgctatct ggagttcctg
    14341 aaaggttatc tgatcccgaa atatgccgag tttatcggca aagagatcca gacggcctac
    14401 cttgaatcct attccgaata tgggcaaaac attttcgacc gttatgttac ctacgcggat
    14461 ttctggattc aggatcagga gtatcgcgat ccggataccg ggcagctgtt tgaccgcgag
    14521 tctcttaacg ccgagctgga gaaaatcgag aaaccggcgg ggatcagtaa tccaaaagat
    14581 ttccgcaacg agattgttaa cttcgtactg cgcgccagag cgaataacag cggacgcaat
    14641 ccgaactgga ccagctatga aaaactgcgc acggttatcg agaagaaaat gttctccaat
    14701 accgaggagc tgttgccggt tatctcgttt aacgccaaaa cgtcaaccga cgagcagaag
    14761 aaacacgacg actttgtcga ccgtatgatg gaaaaaggct acacccgtaa acaggtgcgt
    14821 ttactgtgcg aatggtattt gcgcgtacgt aaatcgtctt aacaaccctg gcccggtaca
    14881 catgctaccg ggcctataac gacagcgaac cgtgggccag agaaatggca acacaggcgt
    14941 agcatacagt tggcaaatgt agtacggggg gcatatgacc gggtttattg accggcgtct
    15001 gaacggcaaa aacaaaagca tggtgaatcg ccagcgtttt ttacgccgtt ataaagcgca
    15061 aattaaacag tcgatctccg aggccattaa taagcgttcg gtgactgacg tcgacagcgg
    15121 cgaatccgta tccattccca cggaagatat tagcgaaccg atgtttcatc aggggcgtgg
    15181 cggcctgcgc caccgcgtac atccgggcaa tgaccatttc gtccagaacg accgaattga
    15241 acgtccccag ggtggcggcg gaggttccgg cagtggtcag ggccaggcca gccaggatgg
    15301 tgaaggtcag gatgaatttg tctttcagat ttcgaaagat gagtatcttg atctgctctt
    15361 tgaagatttg gccttaccga atctgaaaca aaaccaacaa cgccagctga ccgaatataa
    15421 aacgcatcgg gcgggttata ccgctaacgg cgttccagcc aatatcagcg ttgtgcgttc
    15481 cttgcagaac tcactggcgc gacgcacagc catgacggcg ggcaagcgac gggaacttca
    15541 tgcgctggaa gagaatttgg ccatcatcag caacagtgaa cctgcgcaac tgctggaaga
    15601 ggaacgtctg cgcaaagaaa ttgcagaatt acgtgccaaa attgaacgcg tcccttttat
    15661 tgacaccttc gatttacgtt acaagaacta cgagaagcgg cccgatccct ccagccaggc
    15721 agtgatgttt tgcctgatgg acgtttccgg ttcaatggat caatccacta aagatatggc
    15781 taagcgtttt tatattctgc tgtatctgtt cctcagcaga acgtataaga acgtggaagt
    15841 cgtatacatc cgccatcata cccaggcgaa agaagtcgat gaacatgagt ttttctactc
    15901 gcaggaaaca ggcggcacca ttgtttccag cgccctgaaa ctgatggatg aggtagtgaa
    15961 agagcgttat aacccggcac agtggaatat ttacgctgca caagcatcgg acggcgataa
    16021 ctgggccgat gactctccgc tttgccatga aatcctggcg aaaaaattat tacctgttgt
    16081 tcgttattac agctatatcg aaattacccg tcgtgcacat cagacattgt ggcgagaata
    16141 tgagcatctg caatctactt tcgacaactt tgcgatgcag cacatccgcg accaggatga
    16201 tatttatccg gtgttccgtg aactgtttca taaacaaaat gcaacagcta aagactaaaa
    16261 ctatcagcca ggtcattatc gcctggctga tttttagctt actgtaaatt atctcattta
    16321 ttacatacat tagcttacaa tcgctttaaa tatgacagca taacctttac ataatttagt
    16381 tccagaaaac aatcattcgg aaaaatgatt cagtcaacac gtatttccat ggggttattc
    16441 tttaaatatt ttttatcgtt aacgaaaatt gatcctggtc aaaactatat atctctgcca
    16501 tcaataaaat ccagcactca cattgctctc ctttttatgg tttctatggg tacacaaaaa
    16561 ttaaaagctc aaagcttttt tattttcagt ttattgctga cgttaatttt attttgcatt
    16621 actaccttat ataacgaaaa cacaaatgta aaactcatcc cacagatgaa ttacctgatg
    16681 gttgttgtgg ctttgttttt ccttaacgcc gtcatttttc ttttcatgtt aatgaaatat
    16741 ttcactaaca aacaaatttt accaacactc attttaagcc ttgcattttt aagtggcctt
    16801 atctatttag ttgaaaccat tgtaattatc cataaaccaa ttaacggcag tacactgatc
    16861 cagacaaagt cgaatgatgt ttctattttc tatattttcc gccaactcag ttttatttgt
    16921 ttaacctcgc tggcactctt ttgttatgga aaagacaaca tccttgacaa caataagaaa
    16981 aaaacgggaa tcctgttgct ggcgctgatt ccttttttag tttttcccct tctggcacac
    17041 aatctgagca gttataacgc tgactattct ttgtatgtcg tcgactactg tccggacaac
    17101 catactgcga cctggggaat caactataca aaaatattgg tttgtctgtg ggcattttta
    17161 ctgttcttta ttatcatgcg tacacgatta gccagcgaac tatggccgtt aatagcatta
    17221 ttatgtctgg catcgctatg ctgcaactta cttctactga ctctggatga gtataattac
    17281 accatctggt atatcagtcg cgggattgaa gtttccagta aactgtttgt tgtgtctttt
    17341 ctgatttata acatttttca gaaactgcaa ctctccagca aactggcagt tcatgatgtg
    17401 ctgaccaata tttataatcg gcgctacttt ttcaacagcg tagagtcatt attgtcgaga
    17461 cctgttgtta aggacttctg tgtcatgctg gttgatatta atcagttcaa acgcatcaat
    17521 gcccaatggg gacatcgtgt gggtgataaa gtgctggttt caattgtcga tattatccag
    17581 caaagcatcc gccccgatga tattttagcg cgactggagg gtgaggtgtt tggcttgcta
    17641 tttaccgaac tcaatagtgc ccaggcaaaa atcattgcgg aacgtatgcg taaaaatgtc
    17701 gaactcctga ccggctttag taacagatat gatgttcctg aacaaatgac catcagtatt
    17761 ggcacggttt tttcaacggg tgacacgcgt aatatctcgc ttgtcatgac ggaagcagat
    17821 aaagccttac gcgaagcgaa aagcgagggg ggcaacaaag tgattattca tcatatttaa
    17881 gtgcaaaaat attcagagcc atgctttttg cgtggctttt gcatacaatt tattactatt
    17941 acccctaaat ttcccctccc cagcggcgca gagatgagta taattagcgc ccctgtgcca
    18001 ggccgcaatc gaactttatc tggttttctc gtttcactaa ccgaaggagt gccatttatc
    18061 atgaaattgc accatagaat gctccggcat tttatcgccg caagtgtcat tgtgctgaca
    18121 tcttccttcc ttatttttga acttgtcgcc agcgacagag caatgagtgc ctatctgcgc
    18181 tatatcgtgc agaaagcaga ctcctccttt ctttatgata agtatcagaa tcagagtatt
    18241 gccgcgcatg tgatgcgcgc tctcgctgct gagcagtcgg aagtgtcgcc agaacagcgg
    18301 cgcgccatct gcgaggcttt tgagtctgcc aataataccc atggcttaaa cctgactgcc
    18361 cataaatacc cgggcttacg cggcacacta caaaccgcat ccactgactg cgacacaatt
    18421 gtggaagctg cagcactatt acccgctttt gatcaggcag tggaaggcaa ccgccaccag
    18481 gatgattacg gttcaggtct tgggatggcc gaagagaaat ttcactatta tctcgatctc
    18541 aatgaccgct atgtctattt ttatgagccg gttaatgttg aatactttgc gatgaataac
    18601 tggtccttcc tgcagtcagg aagtattggc atcgatcgca aagatattga aaaggtattt
    18661 accgggcgta ccgtattgtc gagcatttac caggatcagc gtactaaaca gaacgtgatg
    18721 agtttgctga cgccggtata tgtcgcaggg cagctaaaag ggattgtgct gctggatatt
    18781 aacaaaaaca atttgcggaa tatcttttac actcatgacc gccctctcct ctggcgtttt
    18841 ctcaatgtca cgctaaccga taccgattcg gggcgcgaca ttatcatcaa ccagagcgaa
    18901 gataatctgt tccagtatgt cagttacgtc catgacttac cgggcggcat tcgtgtctcg
    18961 ttatccattg atattcttta ctttatcacg tcttcgtgga aaagcgttct gttctggatt
    19021 ttgacggcgt taattttgct gaatatggtc cgaatgcact tccgtttata ccaaaatgtg
    19081 tcgcgagaaa atattagtga tgcgatgact ggactgtata atcgcaaaat tttaacccct
    19141 gaactggagc agcggttgca gaaactggtg caatccggtt cttcggtgat gtttattgca
    19201 attgacatgg acaagttaaa gcaaataaat gacaccctcg gtcatcagga gggggattta
    19261 gcgattacgt tattagctca ggcgattaaa caatcgattc gtaaaagtga ttatgccatc
    19321 cgactcggtg gcgatgaatt ctgcatcatt cttgtcgatt cgacgccgca aattgcagca
    19381 caactgcctg aacgtatcga aaaacgtctg caacatatcg cgccgcagaa agaaatcggc
    19441 ttctcttccg gtatttacgc gatgaaagaa aacgatacgt tacatgatgc gtataaagct
    19501 tccgatgagc gtttatatgt caataagcag aacaaaaaca gccgttcatg ataaccttct
    19561 gtggttgttt gcttgtaatc tcaggagcgt gaaatgactg aaatggctaa aggaagcgtg
    19621 acgcatcagc ggttaatcgc tttattatca caagaaggtg ctaacttccg cgtcgttacc
    19681 catgaagcgg taggtaaatg tgaagcggtg tcagaaattc gtggcaccgc tttaggccag
    19741 ggagctaagg cactggtatg taaagtcaaa ggcaatggcg taaatcaaca tgttctggcg
    19801 atcctcgctg ccgatcaaca ggccgatctg agccaacttg ccagccatat aggcggatta
    19861 cgcgcttcgt tagccagccc tgctgaagtc gatgaactca caggctgtgt cttcggcgcg
    19921 atcccccctt tcagcttcca tccaaaactc aaactggttg ccgaccctct actcttcgaa
    19981 cgatttgatg aaatcgcctt caatgcaggg atgctggata aatccgttat tttgaaaacc
    20041 acggattatt tgcgcattgc gcaaccagaa ctgctcaatt tccgccgcac tgcgtaacta
    20101 gcctgtccgt tcaataagca gaacggacaa aaccagcaca acagcaatgg caaaaaacga
    20161 tgacgtgata atcagtgtct cgacgaacat ttgatcgttc atagcatgcc cccgggagat
    20221 agctgttttc acgttattgt tagcgtgcac aaatggcagt ttgatgacaa ttcgcctgat
    20281 tttttatgca aaaaacgtaa agattttcta cttccttcct gcagcaaacg taaagtaagc
    20341 aggcttatta ttttttggca aggaaaccac gatgtttgat gtcactttgc tgatcctgct
    20401 cgggttagct gcgctgggct ttatcagtca taacaccact gtcgccgttt caattctggt
    20461 gttgatcatt gtccgcgtca caccgttaag cacctttttc ccgtggattg aaaaacaggg
    20521 gctaagtatc gggataatca ttctgacgat tggcgttatg gcccccatcg ccagcgggac
    20581 gctaccgccc tcaaccttga ttcactcgtt tctgaactgg aaatcgctgg tggcaattgc
    20641 agtaggcgtg attgtctcct ggctgggtgg gcgcggcgtg accttaatgg gcagccagcc
    20701 gcaactggtc gccgggctgc tggtaggcac tgttttaggc gtggcgctgt ttcgcggcgt
    20761 accggtcggc ccgcttattg ccgcgggtct ggtttcgctg attgtgggga aacagtagtt
    20821 aatcccgcaa tatatcggcc tggcgtttgc cccaggcctt tcttaaacat agtaataaag
    20881 gccgttgttg aatcataccc cagcgtatga gcaactttct gtacagtatc gcctttcact
    20941 aatccctgaa gtgccataat cagctgtaac tgctgacgcc actggcgaaa actcaacccc
    21001 gtctctttta ctatcagtcg cgcaagattg cgttcactca tcgcaaaaaa gccagcccat
    21061 tgccctaatg acccccactc gacgggccct ttcgccatca tctccaccat cgtgcggatt
    21121 ttaggatgag aagaaacggg caggtgcagc tgttgttgtg gctgctgagg gagttcatca
    21181 aacaggacct gaattaatct gcgggtcatc ggctcagcac gctgagtcgt cgtcctgttt
    21241 gccagggtta aaatcaattc ccggcataag ggcgatattt tcaatgtgca acatgttgtc
    21301 ggcattgtca ctgcgctggg ttcgataaac agaaaacaga gttcagcgtt agctgtcact
    21361 tgattgctat gttccacccc gcccggtatc caaaccgcat attgcggcgg caccatccac
    21421 aaagcatttt ccaccgtaca ggtaattgca ccatgtaatg ctagtatcag ctgtcctttg
    21481 cggtgctgat gtacaggact ggttaactca tccgttccgg cgtgaataca aaatgcgacc
    21541 gcggcttcat ggtgtcggtc gggttcatag ccattgagat tcaacctgtg catcattttg
    21601 tccgaattta gcgataattt gtcattttag cttgattcaa cataacaata aaaacggtaa
    21661 ggtacagcct cgtttgtaac aatgagaagc atatgacctg ttcaacttca ttaagcggca
    21721 aaaacaggat tgtccttatc gctggcattc tgatgattgc cacaacatta cgcgtcacct
    21781 ttatcggcgc agcaccgtta ctggatacga ttcgttccgc ttactcgctg acgacagcgc
    21841 aaaccggctt attgaccacc ctgccattat tggcctttgc gctaatctca cctttggcca
    21901 ccccggtagc gcgacgtttt ggtatggaac gtagcctgtt tgccgcgtta cttttgatct
    21961 gtgctggtat cgcaattcgc tctctccctt cgccttactt attatttggc ggtacagcgg
    22021 tcattggcgg tgggattgca ttaggcaatg tcttactgcc aggattaatt aaacgcgatt
    22081 tccctcattc cgtcgccaga cttaccggcg catattccct gacaatggga gctgcagcgg
    22141 cactgggatc ggctatgata gtgccgctgg ctttgaacgg ttttggctgg caaggcgcgt
    22201 tgctcatgct gatgtgtttt cctctgctgg ctcttttttt atggctgcca cagtggcgaa
    22261 gccaacaaca tgcaaatttg agtacctcgc gcgccttaca tactcggggt atctggcgtt
    22321 cgccgcttgc ctggcaggta acattgtttc ttgggatcaa ctcactgatc tattacgtga
    22381 ttattggctg gcttccggcg atcctcatca gtcacggcta tagcgaagca caggcgggtt
    22441 cactgcatgg tttgctgcaa ctagccacag cagctcccgg tttgctgatc cctcttttct
    22501 tacatcatgt gaaagatcag cgtggtattg cagcgttcgt tgccttgatg tgcgcagtgg
    22561 gcgctgttgg gctctgcttt tcggttttgg ttccggcgca acaatgatac tggggttgac
    22621 gttcattggt cttcgggcaa gttctgcgca tcaggcggcg gcactctcgg ggatggcaca
    22681 atccgtcggg tatttgctgg cagcctgtgg gccgccgcta atgggtaaaa tacacgatgc
    22741 taacggtgac tggtccgtac cactgatggg tgttgcaata ctttcactac tgatggcgat
    22801 tttcggactt tgcgccggga gagacaaaga aattcgctaa tatccggtgc tatagtgacg
    22861 tgacaaatca tgcgtgaaag ggagaacaaa cacgatgaat attcagtgca aacgcgttta
    22921 tgatccggct gaacagagcg atggttatcg cgtactggtc gaccgcctct ggccgcgcgg
    22981 tatcaaaaaa accgatttag cccttgatga gtgggataaa gaaatcacgc cgtcaacgga
    23041 actgcgcaaa gcctttcacg gcgaagtcgt cgattttgca acctttcgcg agcaatatct
    23101 tgcagaactg gcgcaacacg agcaagaagg aaagcggctg gcggacatcg ccaaaaaaca
    23161 gccgctgacc ctgctctacg cagcaaaaaa caccacgcag aaccatgcgc tggtgctggc
    23221 cgactggcta cgtagcttgt gattttagta cagcatccgg cggttatttt tcaccagccg
    23281 gatggtcccg tcgccacaat gcccattcat caatcgtttc accgcccggt aatttgcaat
    23341 tgttgctgac cccttgcgct gtcgacactg gaatgagcgt cccgcccttc tgctggcaat
    23401 agaccgacgc cggatttgcc ataccaatct gcggcggttt aggtgcttct ggctgagaag
    23461 aggttgaaca accagccagg accagcaagc aaggcaaagc aaaactgata attttcattt
    23521 attgatctca ctcatttatc tacgcttaga gtatcgcggt atcgttttgt tttgcagcac
    23581 tatttttatt acattcactc aaaacatatt acgtcttgtt tcatctttgt tggtgatgtt
    23641 ttatcatgcc tgcaaagatt aaataatcag catttacccg ccgtatcctg gagttgtttc
    23701 gtgtcagatc agattatcgc ccgcgtctcg caatcccttg ccaaagaaca gtcactggaa
    23761 agtctggtcc gacagcttct ggagatgctg gaaatggtca ctgatatgga atcaacctac
    23821 ctgaccaaag tggatgtcga agcgcgcctg cagcatatta tgtttgcccg taacagccag
    23881 aaaatgcaca tcccggagaa ttttaccgtc tcgtgggatt actcgttatg caaacgcgcc
    23941 attgatgaaa actgcttttt cagcgatgaa gtccctgacc gttggggcga ctgtattgcg
    24001 gcacgcaatc ttggcatctc cacatttctg agcacaccaa ttcacttacc ggatggatca
    24061 ttctatggca cgctttgcgc cgccagcagt gagaagcgcc agtggagtga acgcgcggaa
    24121 caggttttac agttattcgc cggactgatt gcacaatata ttcaaaaaga ggcactggtt
    24181 gaacagttgc gcgaagccaa tgctgcgctg attgcgcaat cgtataccga ctcgttaacc
    24241 gggttgccga atccgcgggc gatttttgaa aatctgacga cgctgttttc tctcgcccgg
    24301 catcttaacc ataagataat gatcgcgttt atcgatctgg ataacttcaa attaatcaat
    24361 gatcgttttg gtcataatag tggcgatctg tttctcattc aggttggcga gcgccttaat
    24421 acgctccagc aaaatggcga agttattggt cgtctcggcg gtgatgagtt tttggttgtt
    24481 tcactaaaca acgagaatgc ggatatttcg tcgctgcgag aacgcattca gcagcaaata
    24541 cgtggagaat atcacttagg tgatgttgat ttgtattatc ccggtgccag tcttggcata
    24601 gtagaagtcg atcctgaaac aaccgatgca gacagtgccc tgcatgctgc cgatattgcg
    24661 atgtatcagg agaaaaaaca caaacagaaa acaccttttg tcgcgcattc agcgccacat
    24721 tgctgaggcg tattcacatc cttttgattg gtgataacat gcgaatcggt attatttttc
    24781 cggttgtaat ctttattaca gcggtcgtat ttttagcatg gttttttatt ggcggctatg
    24841 ctgccccggg ggcataaaga tgaaaaaaac aacgattatt atgatgggtg tggcgattat
    24901 tgtcgtactc ggcactgagc tgggatggtg gtaacgtcac ctctaaaaaa tagcaaaggc
    24961 tgcctgtgtg cagcctttgt gcaatttaag cgttaacttt taatcttcct gtagataaat
    25021 agcacgacaa tcgcaccaat aacggcaacc acgaagctgc caaaattgaa gccatcgact
    25081 ttaccaaagc caaacagcgt gctgatccat ccgccgacta cggcaccgac tatccccagc
    25141 aggatagtca taaagaatcc accaccatct ttacctggca tgatccactt cgccagaata
    25201 ccggcaataa gcccaaaaat aatccatgac agaatgccca ttgtttcctc acttatctgt
    25261 tttgcattag cgggttagtc gctgataaaa agcatagcac aacatcggga gggcaagatt
    25321 tgtgacgagc atcacggagg atttttttgc gatggcgcag aaattgcgcc atcaacgatc
    25381 agtgataatt accaaccaca aacatcatgt tcgttttccg tgtcataaga acgcacggta
    25441 ttcaccagat cttttatcac ttcagccgcc acttctggca ccagcaaagt catcggcgtc
    25501 tctgtttcat aatcgacaga aacgccatta ctgttattgg tgacggtcac ggtatacgtt
    25561 gctttgccca tgattcattt cccgttatga atgactttcc gttgttgcgc accttccatc
    25621 aggacttcag gagccacgaa gaagtcaatg ttgaaataag tatcgtcagt catggcttca
    25681 atgttgtgcc acttttctgg agggaacacc tcaaactgcc ccgcttcgat aaggatcacc
    25741 tgatcaggct ctgcactgtg ttcatcagcg tagccgagat atttgaccgc cccatgcata
    25801 acggaaaggc gtgggtaaac ccccgggcgc gttcctttat caagatgacg ttcgaatatt
    25861 ccggcaggcg cagtttgttt attccagaaa ggcgttgagc gcgtatgaat ataattctgt
    25921 gggatttgca gcatcctttt ccctccttcg gtgaatgcgc tgaaaacggt ttattccagc
    25981 cgtttcaggg tacgcctgat aatttgcatt ttaaatacca tttattggtt actttttagc
    26041 accatatcag agaaaaatca gggaggatta caagtgggaa gcccgcgaca acgcgggctg
    26101 aatgtatcag gattgcagcg tcgccagtcg ggcggcgaaa cccacgaaca tcaaaccaat
    26161 cagtgagttg ccaactttag ccagtttctt tttggtacgt atgtactgcg tgacaaaagc
    26221 accagaaata atcaggaagc tcaaatagca gaaactcacc agttccagcg tcgtcgccag
    26281 aataaagaat gaaattcccg tatgtggggc attaacatcg ataaactgta cgaaaaacga
    26341 cacatagaac aaaatggctt tcggattagt caggctcaaa attaacgcgc gtttaaaaat
    26401 ggccccgtat tggggctcat cggatttggc ctcgttattt ttacccttca gggtcgcgta
    26461 gagaatttta ctccccagat agagcaaata aaacgcacca agataacgta caatgttgaa
    26521 taatatcggg gtggtcttaa ttaatgtcgc cactccagcc catgccagaa acatcaatac
    26581 tgcatcgcca ataaatacac cgcaggccgc aagataaccg cctttcatac cgctactgac
    26641 gctatttttg agtacaaaca gggtatttgg ccctggcacc aacacaataa aaatggcccc
    26701 aaccagatag gtccagtaat tcagaacccc gtattcagcg aacacattaa cctctttaat
    26761 tatctttcga tcatgcgcga ttaaaggtga atatgctaac caatctgtag cggcttagaa
    26821 aggagaaaat caggttttaa cccgatatca acccgataat tgaataatta atcaggcatg
    26881 ctccagtgaa aaattcgggt agtgctcggc aaaatactgg cgtaaaaatt ctatcgttat
    26941 ccgcactttc gctgacgtcg ccagccttga aacataaacg gcccagacgt tcgctggctg
    27001 gtaatattcc ggtagcactt gcactaaatg accactggca atgttttcgc taacatccca
    27061 ccaggagcgc agagcaatcc cctgcccatc gagacaccac tgatggacaa tttctccatg
    27121 attagacgac agtgggccgg tgactttaat cgcatggtgg ccttctttat tgcgtaattg
    27181 ccagacacca aaaggatgat cgcgctcttt aatgaccagg cacggcaatg cggaaagatc
    27241 ggttaaatgt ttcggcgcgc catgttgcgc aataaattcc ggcgacgcgc agagaatgcg
    27301 gtaattagtc gccagtttgc gggcgattaa attaggggcg atgtcatcgc caatgcgaat
    27361 atcgagatca acaccttcat tgaccaaatc gaccaaccgg tcttccacat caaaacgtaa
    27421 ttccagttgc ggatacgcct tagccagcgc tgataacgcc ggagccacca cctgtcgtcc
    27481 aaaaccaaag ctgctgataa tacgcagcat cccctgcgga cctgacgcac gtcagaaagt
    27541 tcgtccatca tctgaccgac atcctgcaaa atccgctgcg cccattcata aatccgctct
    27601 ccttcttcgg taatggtgac gcggcgggtg gtgcggtgta acaacaccac gtttagcgtt
    27661 tgctccagca aggcgatgcg cttgctgacg aacgccggtg aaacgcccag ttcttccgcc
    27721 acggcggcaa aaccagcccg gcgagccacc agcataaaga cgcgcaaatc gttcagcagc
    27781 ggtaaattat tcatgattcg tgttttatgt ttcaccagtt acggggatta attccttttc
    27841 agtcaattat aggatggtga tgttgtcaat tttgatggtc aggaagtgag aacccaatga
    27901 tgaaaacgat gcgtattgct gcgatcccgg gagatgggat tggcaaagaa gtccttcctg
    27961 aagggattcg cgtattacag gctgccgctg agcgctgggg cttcgacttg agttttgagc
    28021 aaatggagtg ggcgagctgc gagtattaca gccatcacgg taaaatgatg ccggacgact
    28081 ggcatgagca acttagccgt ttcgacgcca tctattttgg tgccgtcggc tggccggata
    28141 ccgttccgga ccatatttcg ttgtggggtt cgctgctgaa atttcgtcgt gaattcgacc
    28201 agtacgtcaa cctgcgcccg gttcgtctct ttcctggcgt tccctgcccg ctggcgggaa
    28261 aacagcctgg cgacatcgat ttttacgtgg tcagggaaaa caccgaaggc gaatattcct
    28321 cgctcggcgg tagagtgaat gaaggtacag agcatgaagt cgtcattcag gaatcggtat
    28381 ttacgcgtcg tggtgtcgat cgcattttgc gttatgcctt cgaacttgcg caaagccgcc
    28441 cacgtaagac gctaacttct gccactaaat caaacggttt agccatcagc atgccgtact
    28501 gggatgagcg agtggaagca atggccgaga attacccgga gatccgctgg gacaagcagc
    28561 atattgatat tctctgcgcg cgttttgtga tgcagccgga acgcttcgat gtggtggtgg
    28621 cgtccaattt gtttggcgat atcctttccg atcttggccc ggcctgcacc ggcaccattg
    28681 gcattgcccc atccgccaac ctgaatccgg aacgcacttt cccgtcgctc ttcgagcctg
    28741 tccacggttc cgcgccggat atctacggga aaaatattgc taaccctatc gccacaattt
    28801 gggccggtgc gatgatgctc gattttctcg gcaatggcga tgagcgtttc cagcaagcgc
    28861 ataacggtat tctggcagcg attgaagaag tgattgctca cgggccgaaa acaccggata
    28921 tgaaaggcag tgccaccacg ccacaggttg ccgacgcgat ttgcaaaatt attttgcgtt
    28981 aaggtcaaac cagtttattt gaaccgcgtc actgacgcgg tttttttatt cgttctttgc
    29041 agttaataac ctgcgtcatt tcacctttta ttgtttccgt ttcgtgtttt atggctttcc
    29101 gtattcttaa ttgtttaatt tatgtaacat gcaaataatc aatttgttaa acacaacata
    29161 caactcatta ccctgcaaat aattacaact ggcaaaagcc agagacgggc ttttatgccc
    29221 aggagatgac tcatgatgag caatgttaag aaaaaagatg tgccgctgat aagcatcagc
    29281 ctggtggcca ttcttttcat cgcagctgca ttaagccttt tcccacaaca atcggccgac
    29341 gcggccaaca caatatacac ttttgttacg cgtacgttag gttccgccgt acaggtattg
    29401 gttttgctgg caatgggact ggtgatttat ttagccacca gtaaatacgg caatattcgt
    29461 cttggcgaag gaaaaccgga atacagcacg ctctcctggc tgtttatgtt tatttgtgcc
    29521 ggtttaggtt cttctacgct ttattggggg gttgctgaat gggcctatta ttatcaaacg
    29581 cctggattaa atatcgcacc acgttcacaa caggcactcg aatttagcgt cccttactcc
    29641 ttcttccact ggggcatcag cacctgggca acttatacgc tggcctcatt aatcatggct
    29701 tatcactttc atgtgcgtaa aaacaaaggt ctgagtcttt ccggcattat tgccgcgatt
    29761 accggcgttc gcccgcaagg cccatgggga aaactggttg atttgatgtt tctgatcgcc
    29821 actgtcggcg cactgactat ttcccttgtt gtcaccgcag caacctttac tcgtggactt
    29881 tccgcgctga ccggtttacc cgataacttc accgtgcagg catttgtgat cctgctttcc
    29941 ggcggcattt tttgcctaag ctcgtggatt ggtatcaaca acggtttgca acgtctgagc
    30001 aaaatggttg gctggggcgc gttcctgctg ccattactgg tgctgattgt cggtccaacc
    30061 gaatttatta ccaacagcat catcaatgcc atcggcctga ccacgcaaaa cttcctgcaa
    30121 atgagcttat tcaccgatcc gcttggcgat ggttcattta cccgcaactg gaccgttttc
    30181 tactggctgt ggtggatctc atacacccct ggcgtagcaa tgtttgtcac ccgcgtttcc
    30241 cgcggtcgta agattaaaga agttatctgg ggactgatcc tcggcagcac cgtcggttgc
    30301 tggttcttct tcggcgtaat ggaaagctat gccattcatc agtttatcaa tggcgtaatc
    30361 aacgtcccac aggtgctgga aacactgggc ggcgaaacag ctgtacagca agttctgatg
    30421 tcgttgccag ccggtaaatt gttcctcgcc gcatacctgg gcgtgatgat tattttcctt
    30481 gcctcgcata tggatgcagt ggcctacacc atggcggcga ccagtacgcg taatctccag
    30541 gaaggtgacg atcctgaccg tgggctgcgt cttttctggt gcgtggtgat cactctgatc
    30601 ccgctttcca tcttgtttac cggtgcttcg ctggaaacga tgaaaaccac cgtcgtgctc
    30661 acagcccttc ccttcctcgt cattttactg gtgaaagtcg gcggatttat tcgctggctg
    30721 aaacaggatt atgccgacat tccggctcat caagttgaac attatctccc gcagacaccg
    30781 gttgaagccc tggaaaaaac accagtgctc cctgcgggaa ccgtattcaa aggcgacaac
    30841 tgagcgccat catcctaacg ataaaggtat ccctatgagc aatctgagcc ctgactttgt
    30901 actacccgaa aatttttgcg ctaacccgca agaggcgtgg accattcctg cccgttttta
    30961 taccgatcag aacgcgtttg aacacgaaaa agagaacgtc ttcgccaaaa gctggatttg
    31021 cgtcgctcac agcagcgaac tggcgaatgc caatgattat gtgacgcgtg agatcattgg
    31081 cgaaagcatc gtgctggtac gcggtcgtga taaggttttg cgcgcgttct ataacgtgtg
    31141 tccgcaccgt ggtcatcagt tgttgagcgg tgaaggaaaa gcaaaaaatg tgattacctg
    31201 cccatatcac gcatgggcat tcaaactcga tggcaatctg gcccatgcac gtaactgcga
    31261 aaacgtcgcc aatttcgata gcgacaaagc gcaactggtt ccggtgcgtc tggaagaata
    31321 tgccggattc gtcttcatca acatggaccc caacgccacc agcgtagaag atcagttacc
    31381 cggtctgggc gcgaaagtgc tggaagcctg cccggaagtc cacgatctga aactggcggc
    31441 ccgctttacc acccgcacgc ctgccaactg gaagaacatt gtcgataact atctcgagtg
    31501 ctatcactgt ggtccggcgc atccaggttt ctccgactcc gtacaggttg atcgctactg
    31561 gcacaccatg cacggtaact ggacgctgca atacggtttc gccaaaccgt ccgaacagtc
    31621 gtttaaattt gaagagggta cggatgcggc attccacggt ttctggctgt ggccgtgcac
    31681 gatgctgaac gtcaccccga tcaaagggat gatgacggtc atttatgaat tcccggtgga
    31741 ttctgaaact accctgcaaa actacgatat ttacttcacc aatgaagagt taaccgacga
    31801 gcaaaaatcg ctgattgagt ggtatcgcga tgtgttccgt ccggaagatt tacgtctggt
    31861 tgaaagcgta cagaaagggc tgaaatcgcg tggctatcgt ggtcaggggc gcatcatggc
    31921 cgacagtagc ggtagtggca tttccgaaca tggtatcgcc catttccata atctgctggc
    31981 gcaggtgttt aaggactaat gacatcggcg gcggtatttt ccgccgctgg gctgattttt
    32041 gatggagtac agcaatgtca gactatcaaa tgtttgaagt acaggtgagt caggtggaac
    32101 cgcttaccga acaggtgaaa cgcttcacgc tggtggcaac cgatggcaaa ccgctgcctg
    32161 cgttcaccgg aggaagtcac atcattgtgc agatgagcga tggtgataac cagtacagca
    32221 atgcgtattc actactgagt tcgccgcatg acacctcttg ttatcagatt gccgttcggc
    32281 tggaggaaaa ctcgcgcggc ggttcccgct ttttgcatca gcaggtaaaa gtgggcgatc
    32341 ggttaacgat ttcaacgcct aataacctgt ttgcgctaat tccctcagcc agaaagcatc
    32401 tgtttatcgc gggcggtatt ggtatcaccc ctttcctgtc gcacatggca gagttgcaac
    32461 acagcgacgt cgactggcag ctacattact gctcgcgaaa tccagaaagt tgcgcatttc
    32521 gtgatgagct agtccagcat ccgcaggctg agaaagtcca tttgcatcat tcatcaaccg
    32581 gaacacgact ggaattagcg cgattattgg cggatatcga acctggcaca cacgtttata
    32641 cctgtggccc cgaggcgcta aatgaagcgg taagaagtga agctgcgcgt ctggacatcg
    32701 ccgccgatac gctgcacttt gagcaatttg ctatcgaaga caaaaccggc gatgccttta
    32761 ccctggtgct tgcccgttcc ggaaaagagt ttgtggtgcc ggaagagatg actattttgc
    32821 aggttattga aaataataaa gccgcgaaag tggaatgttt atgtcgtgaa ggggtatgcg
    32881 gaacctgcga aacagcaata ctggaaggtg aagctgacca tcgggatcag tattttagcg
    32941 atgaagagcg tgccagccag caaagtatgt tgatctgttg ttcgcgtgcg aagggtaaac
    33001 gcctggtgtt ggatttgtag tttgccaaag ccggatgtgg cgctgagcgc gcccagtccg
    33061 gcttcggaag attttactgc ggatattcct gcaataaatt gtgtaatgct tccgccatca
    33121 gctcaccacg ccagccggaa atcagctccg gcaaattgtt ctgcggtttc agtttccagt
    33181 gccagttcag cagttggttg atttgccgac gcgatgccag caattcggcg ctgatcttat
    33241 gcgtttcgct cacgtcagta atcagcgact taatcgcttt aaacgcttta cgataacccg
    33301 gcatgtccat caggttaagc atcggctgcg gtaaggcctc ttccggcaat gtctgcgctt
    33361 tttccaccag cgccagcagc gttttaccgt gaaagcggat ttcgctaccg gataaaccca
    33421 ggctgtccag ttcgcctaaa cttcccggca tataacgcgc gaccgaccac aaatgctctt
    33481 cacgcacgac aaagttcacc gccagatcac gctcacgagc cttgcgcagt cgccagtcgg
    33541 ctaacagttg cagacaggcc agttggcgtg tgcgtaattg ccaggcattg gtgatatcac
    33601 gccaggcatc ttccggcgca acgacttcct gacgacgcat ttgcatcagg cggcattcat
    33661 ccagcgccgc aggtagccag ccggaggcct ccgtttctac cataagcttg gcggtgatcg
    33721 gtaacagata ccagacatcc gccgctgcgt attcacactg acgttcggtc agcggtctgg
    33781 ccagccagtc ggtgcgcgat tcactcttgt ccagcgtaac gccggaatac tcttccacca
    33841 tggaagcgaa accccatgac atcgggcgtc cgcagaaggc agcaaggatt tgcgtgtcaa
    33901 tcaggggttg tggtaattcg ccaaagacat tgaggaacac ttccagatct tcactgcctg
    33961 catggagaaa ttttgtgatg gacggatcgc gcaggatcgc tttcagcggt gaccagtcgg
    34021 tgatcccgag aggatcaatt agcgccagat gctcaccatc gaaaagttga atcaacccca
    34081 gctgcgggta ataagtgcgc gtacgaacaa attcagtatc ctgggctatc gccggaaaga
    34141 cacggacggc ttcacacaaa gaagccagcg catcgtccgt ggtaatcatt tggtaattca
    34201 aattgtattc tctttagtgg gcgtcaaaaa aaacgccgga ttaaccggcg tctgacgact
    34261 gacttaacgc tcaggcttta ttgtccactt tgccgcgcgc ttcgtcacgt aattctcgtc
    34321 gcaaaatttt tccgacgtta gatttcggta actcatcacg aaactccacc agcttcggta
    34381 ctttgtagcc cgtgagctga cggcagcaaa aggtcaccag tgactcttcg gtaagcgatg
    34441 gatctttttt cactacgaag attttcaccg cttcaccact ggagccggaa ggtacgccaa
    34501 cagccgcgac ttcctgtacg ccaggatgct gcatgacgac atcttcaatc tcgttgggat
    34561 agacgttaaa accggaaacc agaatcatgt cttttttacg atcgacaatg cgcaggaacc
    34621 cttcttcatc catcaccgcg atgtcgccgg tgtgtaacca gccatttttg atgatctcat
    34681 cggtagcatc gggacgctgc cagtaaccca gcatcacctg cggtcctttg acacaaagct
    34741 cacccggttg acccggtggt acttcattat cgtcgtcatc caccagtttg gcttccgtcg
    34801 acggcaccgg caaaccgatg ctaccactat gataatcaat atcatatggg ttaacgctga
    34861 ccagcggcgc acactcggta agaccatagc cttccagcag atactgtcct gtcagtttca
    34921 cccaacgctc tgccaccact tgctgcactg gcatcccacc gcctgcggaa agatgcagac
    34981 tggagaaatc cagctgctgg aactctttat tgttcagcaa cgcattgaac aaggtgttaa
    35041 cgccagtgat agcggtaaac ggatatttcg ctaactcttt taccaaccct ggaatatcgc
    35101 gcgggttagt gataagcagg ttctgcccac ccagttcgat aaacagcagg cagttaatgg
    35161 tcagggcaaa aatgtgatac agcggcagtg ccgttactac cagttcttta cctggatgca
    35221 gcaacggccc ataggtggcg ttgacctgtt ccaggttcgc cagcatattg cggtgagtca
    35281 gcatcgcgcc tttcgccaca cctgtggtgc cgccggtgta ttgcaggaag gctaaatctt
    35341 ccggcaccag ttcgggtttg acgtactgca tccggtagcc gttatgcagt gcgctacgaa
    35401 atgaaatggc atctggcaga tggtatttcg gcaccaaacg cttgatgtat ttaacaacga
    35461 aattgactac cgtgcctttt gccgtagata gctgatcgcc catacgggtc agaattacgt
    35521 gctgaacggc ggttttatca accacttttt ccagtgtgtg agcaaagtta gacacgataa
    35581 caatcgccga tgcgccgcta tcgttaagct gatgctcaag ctcacgcggg gtatacaacg
    35641 ggttaacgtt tacgacgatc atcccggcac gcaaaatgcc aaacagcgcc accggatatt
    35701 gcaataaatt aggcatcatc aacgcaacgc gatcgccttt cttcagcccc aacccttgtt
    35761 gcaaataagc ggcaaacgcg cgactgcgtt cttccagctt gcggaaggtc attacctccc
    35821 ccatattcac aaacgcaggc tgatcggcgt agcggacgac cgactgctca aacatatcta
    35881 tcagagattg ataacggtca gggttgatct ccgtcggaac atccgcggga taacggttaa
    35941 gccaaacctt cttcaattct tcacctctaa aatgcgtgtt cgtcgtcatc gcaaccccaa
    36001 gtgataaaca tgttgttaac ataacattaa ctcatcttac cagcttgata attagacaac
    36061 gaaaaggttg cgaagcgcgt cactatttat ttttatagtt gccgtatgaa cgcagaaaca
    36121 gcggaccagc cgctgtttct ttttctttaa aaacaacttg ttactcggtt acaattgttt
    36181 caactcttgc agggccagga ttataccaac cccatccgcc gtaaccatag ggccagccgc
    36241 gtccaccata aaaccatgga tcaattggct gcggcggcat aatcacctgt tgagtgatat
    36301 gccagcgttt gtaacctgtt acctgcataa ccataaattt atatggtgca ttatttactt
    36361 tgccatccac tgtgccagtg attggcccga caaccgtcac cagttgtcca cgaaaatcga
    36421 ccggatcaag aaaaccgttc acatcggcat aaatgcgccc gcgtgaaggt tctcccaagg
    36481 ttggtcttgc tccgctgtcc agcggtacgg tagcaatttc caggcgggtt ttcccttgct
    36541 ggttttgtac cacaaccact ttgccgccaa agcgcgcctc ctgaccaaca tataaatctg
    36601 gcgcattcat cacgcgtacc agatcttgct gtggtgttgg actgctgcct ttaatggcgt
    36661 ccggcacagt gacacaaccg ctcagcatta gcgcaaacgt acctgccagt atgcctttga
    36721 taacattctt ttgaaccgcc atgatgcgac tccttttttt ctcagggcat actcttaaga
    36781 ttcattcttt gcccggaagt ttcttccatg cgacgttgtt acgtaaataa accggttcgg
    36841 catgttccac cgccaccgtt ttaccctcgg caaacatctg acacgcaatc ggcagcatat
    36901 cttccgccgc tggcagtaaa acttctccgt cgcgcaaaac cagcccgctc tctttacgga
    36961 gatccggcca ggcttgccag cccgtcccta ccgttaccca ttcgccggaa agctgttgca
    37021 ttcgttcatg aacgagttcg ggtttgagta cggcttcggt ttcttcaccg cgccagatac
    37081 cgttttcatc acgctgatat tcggcccagt aaacttcgcc cattcgcgcg tcaatggctg
    37141 ccagcacgcg ggttgcgccg tttttgcgcc acgccccctg cgccatcgtc attagcgtag
    37201 agacaccaat catcggtaac tccgcgccaa gcgccagccc ttgcgcaata ccaataccaa
    37261 tgcgcacgcc agtaaagcta ccggggccgc gcccgtaagc cagagcgttt atgtcagtca
    37321 gggaagttcc gctggtggtc aggatatcct gcaccatcgg taagattcgt tgagtatgtt
    37381 cacgagggca aagctcaaaa tgagcgttga cattaccgtc gttccacaga gcgacagagc
    37441 agacctctgt cgcggtatcg atagccagaa ttcgcatggg tcttcgtgct tagatcaata
    37501 aaaaggcgcg catcatacca tactccgtaa caaattacct ggaggatggt atcgcaagga
    37561 aacgaaccgc acgggcaatg tcacgggtgc gtggcgcggg cggcagactg gcgagaaacg
    37621 tcgcgccgta aggacgcatc accagccgat tgtcacaaat taccagcacg ccgcgatcgt
    37681 cggcgtcgcg aatcagtcgc cctactccct gcttgagagt aatgaccgca tccggcagct
    37741 gcacttcatc gaacgggtcg ccaccgcgca aacgacaatc ttccatgcgc gcttttaaca
    37801 gtagatcatc cggcgaggta aacggcaatt tgtcgataat taccaatgac aatgtatcgc
    37861 cacgcacgtc caccccttcc cagaaactac tggttgccac aagaagcgcg ttaccggcgc
    37921 tgacaaattg ctgcaacagt tgccctttgc tggtttcccc ctgcaacaaa acgggaagcg
    37981 tcatggtagc gcggaactgc tcggccagat cgcgcatcat ggcatgcgag gtacaaagca
    38041 taaaacaacg accgttgtta gcttcgatga tcggtcgcag cattgccgcc agttgccgtg
    38101 cacaacccgg ttgattggtt tgcggcaggt tgcgcggcac acagagtaac gcctggcggc
    38161 tgtaatcaaa cgggcttggc aatagcaacg actccgcctg ttctatccca agccgcgagg
    38221 tgaaatgatg cagatcgtcg ttcaccgaca gcgttgctga ggtgaagatc cagctaccgg
    38281 gcttttgcgc cattaactct ttgaatttat ccgccacgct gagcggcgtg agagccagag
    38341 taaaatggcg cgaagtgcat tcgtaccagt agctgtagcc cggctgattg atctctttta
    38401 gccgcttcag ccgtgtgcga tacaacgtgg cgcgctcaaa tgccgcatcc agcaaggcgg
    38461 aacgccccag tgacagtttc gccacgtcat aacaaagttc cagggtgtca tcgagcagta
    38521 aaaatgcccg ctgaatttgc ggattagcta acagctcacg caggttaccg cgataacctg
    38581 gctcaccgag ttgcagacga aaatcctgtg cactctgggc aagacgatca gcgcactttt
    38641 gtaactgctg ggtgtctttt aattcggtgc ggtaggcgat ggtgatgtct tttgccaggt
    38701 cgagcagttg tcgactggag agtgactgac caaaatactg gctggcaatg tccggtagct
    38761 ggtgggcttc gtcgaagatc atgacgtccg cttcagggat caactcgcca aatccactct
    38821 ctttaaccac catatccgcc agaaagagat gatggtttac caccaccaca tcggcgtcca
    38881 tcgctttttt acgtgctttg accacaaagc aatctttata catcgggcag tcgctgccaa
    38941 gacagttgtc gttggtgctg gtgaccagcg gccacgcctg tgaatcttcc gccacgctga
    39001 cgcaggtgct gatatcacca tcgactgttt gattagacca ggagcgcagc aggatcacat
    39061 cgcttaagat ttgtaccggc agatcgcccc ccgccagcgc ctgctgttcg agacgttcga
    39121 ggcagaggta gtttgagcgc cctttcagca gcgccacgtt gcccgtatat ttcaatgcct
    39181 ttgagactgt tggcaaatcg cggctgtaga gctgatcctg caacgctttt gagccggtcg
    39241 agataatgac tttctttttc gcccgcagcg caggagccag gtaagcgtag gttttgcccg
    39301 taccggttcc tgcttccacc accagcggct ggcctttttc tatcgcctgg gtgacggcta
    39361 ccgccatctg tcgctgtggt tctcgcggct taaagcctgg tatcgctttc gccagctgac
    39421 cgtctggtgc aaaatcgtcc gtcacactac cccctgttga tttgaacagg gattatgtca
    39481 ggatgagggc gctttcgcca gttgaagtgg tgacggcaac ctcacattgt ggcagtcttt
    39541 gcagcacgaa aatggaagta taatgaggac aaaatgatga ctatcgttcg tatcgatgct
    39601 gaagcccgct ggtctgatgt agtaatccac aacaacacgc tctactacac tggtgtaccg
    39661 gaaaacctcg acgccgatgc ctttgagcaa accgccaaca cgctggcaca gattgacgcc
    39721 gtgctggaaa aacagggcag caataaatcg agcattctgg atgccaccat tttcctggcc
    39781 gataaaaacg acttcgcggc gatgaataaa gcgtgggatg cctgggttgt cgcgggtcat
    39841 gcgccagtgc gctgcacggt acaagcgggt ttgatgaacc cgaagtataa agttgaaatt
    39901 aagattgtgg ctgcggtgta agctttatcg aagcaaaata agtcagacga taatttatcg
    39961 ataatactgg tcggttttac ataaatcgac cagagaataa gatattactg ttgatgcttc
    40021 tgctaattca ttatttatat ttataatttc aattttatct ataaaactaa ctatataaat
    40081 aaacataacc atgccgttgt gttcgccctt tgattagcaa ctctggtttt ctttcttagc
    40141 tgaacaatct ggttatagcg tgcggactga ccgttaattt tctccttctt actggataac
    40201 acaccatgag ctgctacctt taaaacacct gaatgtcaac aggttaactc gtgccatatc
    40261 gttataatcc ctttgatatt agatgcaa
//
LOCUS       DAKISA010000032        39729 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.032, whole genome shotgun sequence.
ACCESSION   DAKISA010000032 DAKISA010000000
VERSION     DAKISA010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39729
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.032"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            4..435
                     /locus_tag="OVW45_001702"
     CDS             4..435
                     /locus_tag="OVW45_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCV5526176.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFA
                     VQSGPMLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            518..1420
                     /locus_tag="OVW45_001703"
     CDS             518..1420
                     /locus_tag="OVW45_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HCV5526177.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(1475..3637)
                     /gene="uvrD"
                     /locus_tag="OVW45_001704"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(1475..3637)
                     /gene="uvrD"
                     /locus_tag="OVW45_001704"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCV5526178.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(3721..4437)
                     /gene="yigB"
                     /locus_tag="OVW45_001705"
     CDS             complement(3721..4437)
                     /gene="yigB"
                     /locus_tag="OVW45_001705"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCV5526179.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(4437..5333)
                     /gene="xerC"
                     /locus_tag="OVW45_001706"
     CDS             complement(4437..5333)
                     /gene="xerC"
                     /locus_tag="OVW45_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCV5526180.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(5330..6037)
                     /locus_tag="OVW45_001707"
     CDS             complement(5330..6037)
                     /locus_tag="OVW45_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCV5526181.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(6034..6858)
                     /gene="dapF"
                     /locus_tag="OVW45_001708"
     CDS             complement(6034..6858)
                     /gene="dapF"
                     /locus_tag="OVW45_001708"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCV5526182.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6895..7098)
                     /locus_tag="OVW45_001709"
     CDS             complement(6895..7098)
                     /locus_tag="OVW45_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCV5526183.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            7561..7881
                     /gene="cyaY"
                     /locus_tag="OVW45_001710"
     CDS             7561..7881
                     /gene="cyaY"
                     /locus_tag="OVW45_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCV5526184.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7921..10467)
                     /gene="cyaA"
                     /locus_tag="OVW45_001711"
     CDS             complement(7921..10467)
                     /gene="cyaA"
                     /locus_tag="OVW45_001711"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCV5526185.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            10854..11795
                     /gene="hemC"
                     /locus_tag="OVW45_001712"
     CDS             10854..11795
                     /gene="hemC"
                     /locus_tag="OVW45_001712"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCV5526186.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAKQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            11792..12532
                     /gene="hemD"
                     /locus_tag="OVW45_001713"
     CDS             11792..12532
                     /gene="hemD"
                     /locus_tag="OVW45_001713"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCV5526187.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGQQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNAANDALLRALQ"
     gene            12554..13753
                     /gene="hemX"
                     /locus_tag="OVW45_001714"
     CDS             12554..13753
                     /gene="hemX"
                     /locus_tag="OVW45_001714"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCV5526188.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAAAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQATQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPQGDTP
                     AAAPQGE"
     gene            13756..14952
                     /gene="hemY"
                     /locus_tag="OVW45_001715"
     CDS             13756..14952
                     /gene="hemY"
                     /locus_tag="OVW45_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCV5526189.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            15614..15775
                     /locus_tag="OVW45_001716"
     CDS             15614..15775
                     /locus_tag="OVW45_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5526190.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYPQNAAIY"
     gene            complement(15859..17094)
                     /locus_tag="OVW45_001717"
     CDS             complement(15859..17094)
                     /locus_tag="OVW45_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCV5526191.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAARDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(17241..17317)
                     /locus_tag="OVW45_001718"
     tRNA            complement(17241..17317)
                     /locus_tag="OVW45_001718"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17281..17283),aa:Pro,seq:tgg)
     gene            complement(17360..17446)
                     /locus_tag="OVW45_001719"
     tRNA            complement(17360..17446)
                     /locus_tag="OVW45_001719"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17410..17412),aa:Leu,seq:cag)
     gene            complement(17467..17542)
                     /locus_tag="OVW45_001720"
     tRNA            complement(17467..17542)
                     /locus_tag="OVW45_001720"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17507..17509),aa:His,seq:gtg)
     gene            complement(17601..17677)
                     /locus_tag="OVW45_001721"
     tRNA            complement(17601..17677)
                     /locus_tag="OVW45_001721"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17641..17643),aa:Arg,seq:ccg)
     gene            complement(17780..19165)
                     /locus_tag="OVW45_001722"
     CDS             complement(17780..19165)
                     /locus_tag="OVW45_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCV5526192.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(19356..20096)
                     /gene="rffM"
                     /locus_tag="OVW45_001723"
     CDS             complement(19356..20096)
                     /gene="rffM"
                     /locus_tag="OVW45_001723"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCV5526193.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(20099..21451)
                     /gene="wzyE"
                     /locus_tag="OVW45_001724"
     CDS             complement(20099..21451)
                     /gene="wzyE"
                     /locus_tag="OVW45_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCV5526194.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(21448..22527)
                     /gene="rffT"
                     /locus_tag="OVW45_001725"
                     /gene_synonym="wecF"
     CDS             complement(21448..22527)
                     /gene="rffT"
                     /locus_tag="OVW45_001725"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCV5526195.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(22524..23774)
                     /gene="wzxE"
                     /locus_tag="OVW45_001726"
     CDS             complement(22524..23774)
                     /gene="wzxE"
                     /locus_tag="OVW45_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCV5526196.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(23776..24906)
                     /gene="rffA"
                     /locus_tag="OVW45_001727"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(23776..24906)
                     /gene="rffA"
                     /locus_tag="OVW45_001727"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCV5526197.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(24911..25585)
                     /gene="rffC"
                     /locus_tag="OVW45_001728"
                     /gene_synonym="wecD"
     CDS             complement(24911..25585)
                     /gene="rffC"
                     /locus_tag="OVW45_001728"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCV5526198.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDMLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(25563..26444)
                     /gene="rfbA"
                     /locus_tag="OVW45_001729"
     CDS             complement(25563..26444)
                     /gene="rfbA"
                     /locus_tag="OVW45_001729"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCV5526199.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     DIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(26463..27530)
                     /gene="rffG"
                     /locus_tag="OVW45_001730"
     CDS             complement(26463..27530)
                     /gene="rffG"
                     /locus_tag="OVW45_001730"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCV5526200.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(27530..28792)
                     /gene="wecC"
                     /locus_tag="OVW45_001731"
     CDS             complement(27530..28792)
                     /gene="wecC"
                     /locus_tag="OVW45_001731"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCV5526201.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(28789..29919)
                     /gene="wecB"
                     /locus_tag="OVW45_001732"
     CDS             complement(28789..29919)
                     /gene="wecB"
                     /locus_tag="OVW45_001732"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCV5526202.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(29975..31021)
                     /gene="wzzE"
                     /locus_tag="OVW45_001733"
     CDS             complement(29975..31021)
                     /gene="wzzE"
                     /locus_tag="OVW45_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCV5526203.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(31033..32136)
                     /gene="wecA"
                     /locus_tag="OVW45_001734"
     CDS             complement(31033..32136)
                     /gene="wecA"
                     /locus_tag="OVW45_001734"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCV5526204.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(32376..33635)
                     /gene="rho"
                     /locus_tag="OVW45_001735"
     CDS             complement(32376..33635)
                     /gene="rho"
                     /locus_tag="OVW45_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCV5526205.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(33720..33821)
                     /gene="rhoL"
                     /locus_tag="OVW45_001736"
     CDS             complement(33720..33821)
                     /gene="rhoL"
                     /locus_tag="OVW45_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCV5526206.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(33962..34291)
                     /gene="trxA"
                     /locus_tag="OVW45_001737"
     CDS             complement(33962..34291)
                     /gene="trxA"
                     /locus_tag="OVW45_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCV5526207.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            34422..35687
                     /gene="rhlB"
                     /locus_tag="OVW45_001738"
     CDS             34422..35687
                     /gene="rhlB"
                     /locus_tag="OVW45_001738"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCV5526208.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            35821..37305
                     /gene="gppA"
                     /locus_tag="OVW45_001739"
     CDS             35821..37305
                     /gene="gppA"
                     /locus_tag="OVW45_001739"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCV5526209.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIANAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(37352..39373)
                     /gene="rep"
                     /locus_tag="OVW45_001740"
     CDS             complement(37352..39373)
                     /gene="rep"
                     /locus_tag="OVW45_001740"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCV5526210.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
BASE COUNT         9860 a        10799 c        10037 g         9033 t
ORIGIN      
        1 aaggtggcgt taaatctcgc ttcaggtgaa gggaatttct ttatccgtcc tggcggcgtg
       61 ttttatgtcg cgggagataa agtcggcatc gttcgtctgg atgccttcaa aaccagtaaa
      121 gagattcagt ttgcggtgca gtcagggcca atgttgatgg aaaacagtgt aattaatccg
      181 cgtattcatc ccaacgtcgc ctcacgtaaa attcgtaacg gtgttgggat taataaacat
      241 gggaatgccg tgtttttgtt gagccagcag gcgacaaatt tttatgattt tgcctgttat
      301 gccaaagcga aactgaacgt tgagcagctg ctttatcttg atggcacgat ttcacatatg
      361 tatatgaaag gcggggcaat cccgtggcaa cgctatcctt ttgtcaccat gatttccgtc
      421 gagaggaaag gttaagcatt gtacactcga ggaggccaga caaactgggg ctggctacac
      481 agctggaaaa tcatcagata aatgaaggga aacaaaaatg aaaaaaacgc tcgcaacgtt
      541 atttttatta acgtgtctgg gatcatcatc cgcttatgcg gataacgctt tgattttaca
      601 aactgatttt agcctgaaag atggtgccgt ctcggcgatg aaaggggtcg cattcggtgt
      661 tgaccataat cttaaaatat ttgatttaac acacgaaatt ccaccgtata acatctggga
      721 aggcgcttac cgcctgtatc agaccgccag ttactggccg caaggttcgg tatttgtcag
      781 tgtggtcgat cctggtgttg gtacagaccg taaatcggta gtgctaaaaa ccaaaaacgg
      841 tcaatacttc gtttcaccag ataacggtac gctcactttg gtggcggaat cgctgggtat
      901 tgagagcgtc cggcaaatcg atgaaaaaac caaccgcctg aaaggttccg aaaagtcata
      961 taccttccac ggtcgtgacg tgtatgccta caccggtgca cgcctggctt ccggagcgat
     1021 cacgttcgaa caggtggggc cagagcttcc tgcaaaagtt gtcgaacttt cttatcagaa
     1081 agccaaagca acaaaaggtg aagtgaaggg caatatcccg attctcgata ttcagtacgg
     1141 caacgtctgg agcaatatca gcgacgagtt gctgaatcag gcaggaatca aactcaacga
     1201 cacgttgtgt gtaaccattt ccgaaggttc ccagcaaaaa tacgcaggga aaatgccgta
     1261 tgtcgcaagc ttcggcgatg tgccggaagg ccagccgatg gtgtatctaa acagcttatt
     1321 gaatgtttcc gtggcgctca atatggataa tttcgcgcag aaacatcaag ttgcgtctgg
     1381 tgctgactgg aatattgatg tgaagaagtg cgctaagtaa ggtgctgatg caggatgcgg
     1441 tgtggcgcac cgcatcctgc aacggagttg taaattacac cgtctccagc cgggcgtaag
     1501 ccgccaccag ccatttgatt ccctggccct ggaatgccac ctgcaaacgg ctatgttcac
     1561 cgctgccttc catattgacg atggtgcctt caccaaactt agcgtggcgc acgcgctggc
     1621 cgagtttgta gccgctgtcg ttctcgacca tcggcgtacc catccgctga tggctaaccg
     1681 ggcggcttac cgtggcgcgc aggcgcacct cttccacaca ctcttccggc agctcgccga
     1741 taaagcgcga cgggcgatgg taaacctctt tgccatacag acggcgagtt tccgcgtagg
     1801 tcagcgtcag tttctgcatc gcacgggtta cgccaacgta ggccagacgg cgttcttctt
     1861 ccagacgtcc gccttcatcc agcgacatct ggcttgggaa catgccctct tccataccga
     1921 cgataaacac ctgcgggaac tccagccctt tcgccgagtg tagcgtcatc aactgcaccg
     1981 catcctgcca agtatccgcc tgcccttcac ctgcttccag tgccgcatgg gagaggaacg
     2041 cctgcagcgg cattaaatct tcgtcttctt cgttgtagct gaactggcgc gttgccgtca
     2101 ccagttcctc taagttttcg atacgcgtct gacctttttc gcccttctcc tgctcgtaca
     2161 tggtacgcag gccggagtct ttaattaccc ggtcagtctg tacatgcagc ggcatatcgg
     2221 cagtttcttg cgctaaggca tcgattaatt ccataaatcg ctgcaaggcg ctggcagctc
     2281 gcccggcgag ggctttttcc tgcaacagtt cacgacatgc ctgccagagt gttaactggc
     2341 gatcgcgcga tgtctgacgt accacgtcca gcgtccggtc accaataccc cgcgttggcg
     2401 tattcaccac gcgctcaaac gccgcgtcgt cgttacggtt ggcaatcaga cgcagatacg
     2461 agagcgcgtc tttgatttcc tggcgttcga agaagcgcat cccgccgtaa atacggtacg
     2521 gcatactggc ctgcaacagt gcttcttcca gtacacgcga ctgggcgtta ctgcggtaga
     2581 gaatggcgca ctcggcaagc gccccgccgt tgtcctgcca ggttttgatg cggttaacca
     2641 caaaacgcgc ttcgtcgagt tcgttaaaag cgcaatagag ggaaataggc tcaccgtccg
     2701 cgccatcggt ccacagtttt ttacccagac gcccgttgtt gttttcaatc agggcgttcg
     2761 cggcgctcag aatattgctg gtagagcggt agttctgctc cagacggata gtttcagcac
     2821 cggggaaatc attgaggaaa cgctgaatat tctctacctg cgccccgcgc cagccgtaga
     2881 ttgactggtc gtcatcgcca acgatcatca ctttgccggt atcgcccgcc agcaggcgga
     2941 tccacgcgta ctgaatgttg ttggtatcct ggaattcgtc caccaggatg ttggtaaaac
     3001 gttcgcggta gtgttgcagg atatgcggct tgttaagcca caactcgtga gcgcgcagca
     3061 gcagctcggc gaagtccacc aggcccgcac gatcgcacgc ttcctgatac gcctgataca
     3121 ccttctgcca ggtctgctcc accggattac cgtagctttg aatatgatgc ggacgcaggc
     3181 cttcatcttt ctggctgttg atgtaccaca ttgcctggcg cggcggccac tgcttctcgt
     3241 cgaggttcat ggctttgatc agacgcttaa gcaggcgtag ctggtcttca ctgtcgagga
     3301 tctggaaatc ctgcggtaga ttggcgtcca tatggtgcgc acgcagcaaa cggtgcgcca
     3361 gcccgtggaa ggtgccgacc cacatgccgc cctggctggt gcccatcagt tgcccgatac
     3421 gatgacgcat ctccgccgcc gctttgttgg taaacgtcac agccataatc gagtatggcg
     3481 agcagttttc cacgctcatc agccaggcga tacgatgcac cagtacgcgc gtcttaccac
     3541 tgcccgcgcc cgccagcacc agaaggttgc tgcgtggcgc ggccaccgct tcgcgctgtt
     3601 tgtcattaag gctgtcgagc aggtaagaaa cgtccattgg caccgccgcg taaaaatagg
     3661 ttgggcggag aagcgcaacg ccctccgcca aaaagctggg tatatataca gatttactga
     3721 ttatatcagc gaggtcagag atgccaaccg ggaaatttcc agatgcggca gtaaacggct
     3781 gtcccaggtt tgcatcagat cgccattttc cggtctgatc caacaagcct gcattccgct
     3841 gcgaattgcc ccacccacgt cagtggtgag atcgtcccca acatgtaaga tctcgccgat
     3901 cggcacgttg agtttttccg cagccaaaaa gtacatatcg ctgaacggtt ttgagcgccc
     3961 gtgcgggcca gcgcgcagca caaactcaaa ataatccccc aaaccaaaca gttccggctg
     4021 ggcgttaccg ttggtgatcg ccaccagcgg ccatttcttc gccagctgtt ttaaggtgtc
     4081 gtgagtttgc tgcgggacgt cgattcggct gcgccatttg gcaaagttaa tcattgctgc
     4141 gtgtgcgcct gcactggctt cttcggcact cagcccggcg tcgagcatcg cttgttcaat
     4201 cgaacgaaaa cgccaacgcg tcacgtcgtg ataaatctcg ggttccgctt cccgtaccgc
     4261 ctggcgcagg cgttgcagat cttcattctg gaagctgcgc agcgccggat gataattttg
     4321 cacaaaggta agcgcctctc gctcggtgcg caaaatcacc ggacggttat cgtaaagggt
     4381 atcatccagg tcaaaggtga gcgccgagat gcgccccaaa ggccggtaaa aacgcattat
     4441 ttcccccgtt tggcgcgtgg atgcgccgca tcgtacaccg aggcaaggtg ttgaaaatca
     4501 agatgagtat agatttgcgt ggtggagagg ttggcatgac ccagcagctc ctgcacacca
     4561 cgaagatcgc cgctcgactc cagcatatgc gtggcgaacg agtgacgtaa tttatgcgga
     4621 tgaacgtgat tattcagccc ttgttttatg ccccattcgg caaagcgttt ctgcacatta
     4681 cgcgcggaga tacgcttgcc cagtttcgac agaaaaagcg cgtcgtcttc gctaccaaac
     4741 aggtcgcgca aatcaagcca gtgctcaatc cacgccacag cgttgcgacc aatcggcagg
     4801 cggcgctctt tgctgccttt ccccattacc cacacttcac cagactccag gtcgaggtgt
     4861 ttgatatcca gccccaccag ctcggaaaga cgcagacccg cgccgtacat cacttccagc
     4921 attgcacggt cgcgtacagc gaggggatcg ttgatatcaa tgtccagcag ccgattcata
     4981 tcgtcgacgt cgatattttt cggcagatga cgcggcgctt tcggtgccga aacacctttc
     5041 gccgggttag ctttgagttc gttctggctg accagccagt caaaaaagct acgtagcgca
     5101 gaaagccgta acgcgaggct tgctgctccc agccctttac ggcgactgcg tacagcaaaa
     5161 ttgcgcacca tcgtcacatc acattgctgc cagctttgca ggccgttttc gctggcaaaa
     5221 ttgatgatcg cctcaagctg acgctggtag ttaagcaggg ttatcgggct aagctggcgc
     5281 tccacgctca gataacgtag gtagcgttct acatcggtgt gtaaatcggt catacgcgtt
     5341 caatccaacg ctccagaagc tccggcaaca tcagcgcaat ttcatgaagt aactgcgttc
     5401 cctgcccttg ttgatagtga ctggcatcgc gactggtaaa cagcacgaca cccaaatcag
     5461 catcgcttcc cagcatcgac atcgccaccg atcccaccgc tttcgcttcc ggtagcacca
     5521 ccagcagctc tggtccgtta agcggcccaa gatagtgctg ttcctgcccc aaacgctgaa
     5581 tacgcagcgg ttcgaaagac tgacggctta atgccagatg agtgtggttc gacggcgcac
     5641 ctaagcgcca gcgatccgga aacaggcgca gactcgcacc tgccaggccg agatcgcgcg
     5701 cccagcggtg aaagcgcatc agcatatcgt cgagactgct ggcggcggtg agactgcgct
     5761 gcaggtagag taggcgataa aacaggcctt cgttggcgat agcctgttcc atcaacagcg
     5821 ccatgttctc ttccagaaca tgaatatgat tacgtgcgcg ggccatgtgc cactcgacca
     5881 acgaaacggt gccgcgtacc ggatgcggca cacgtatcgc ttctactgcg cgcgcattac
     5941 ggataaaaaa ctcaggattt ttaatcagat aatcgacaac cgcccggtca tcaagctccg
     6001 tgagtgtttc ctgcagttct tcccctggtt gcttcataga tgaataaatc cgtcgtagac
     6061 atgtaccgcc gggccagtca tatataacgg gtgacccgga cctttccagg cgatatcaag
     6121 acgaccgccg gggagttcca cgcgtacttc ttcggccagc aaaccttgct gaatccctac
     6181 tgcaaccgcc gcacacgcgc cgctgccgca ggcctgggtt tctcctgccc cacgctcata
     6241 aacgcgtaaa cgaatatgct cgcgcttaac cacttgcata aaaccgatat tggcgcgctc
     6301 cggaaaacgc tcgtggcttt ccagaacagg accaagcgtt tctaccgccg cggtatcgac
     6361 atcatcgacc tgaatcacgc aatgcggatt tcccatcgac accacgccgc ataagattgt
     6421 ctgctcggcg gcgcgcataa tataggtctt ttccgctttg ttagcgcgaa acggcacggc
     6481 ggaaggttcg aagttgggtt cgcccatatt tacgcggacc agatcatcat cggtgacggt
     6541 cagaaccatc cgcccgttgg cggtgctgac gcggatatca cgcttattgg tcagtccttt
     6601 cagacgcaca aaacgggcaa agcagcgcgc accgttgccg cactgcgcca cttcactgcc
     6661 atcagcattg aaaatgcgat agtgaaaatc cagttcagga tcatacggcg gctcaaccac
     6721 cagcagttgg tcaaacccta cccccaggtg ccgatcagcc aggcgacgaa tcagctccgg
     6781 tgaaaaaaag acattctgcg ttaccgcgtc gacgaccata aaatcgttgc caaggccatg
     6841 cattttcgag aactgcatca tttactccaa tcacgcgggt acagaaactg acttttagta
     6901 attcacctgg gatggaccat cgccagtggc gcgatcgttt ttatccggca ccgtggattg
     6961 cgtttgcgtc tctaccggtt tggtcggcgg cggtgcgttt ttatctgcag gcgggaaata
     7021 gagcggacct ttcagaccgc agcccgtcag gctgaagaga gtaagtaata cagtgagtgc
     7081 cttaaacacg tttttcatta tggattgcct gtaaagttca tcgctttctg ctttctatca
     7141 tcgcaggaga agacgtaaaa gcaagcgttt agcgactctt tatgaagtct gaaaagtgag
     7201 gaaatgctgc gagtataacg cttactcgtg gggatttatg ttttgctggt agtttcgtga
     7261 tcggcacctc gcgccaggat ggctctatgc actatcgcaa tgtggtttgt ttgttgtctt
     7321 ttttagttcc gtcagcgtga ggatgctcgc tcggtaaacc tgatcataat gtttaccaga
     7381 aacagggaaa ggcgtggtgt aactgcgccc taatgaagag acaaatcttt cgctacacag
     7441 agacatcatt attccggtga cggatgaatc gtgccggaac gcagccgcag aggtgcaaac
     7501 tttgaagccc tgaccccatg ccgctatact gccgccatca cttaaaaaca ggatacaaca
     7561 atgaacgaca gtgaatttca tcgcctggct gatcaactct ggctgaccat tgaagaacgc
     7621 ctggacgact gggatggcga cagcgatatc gactgcgaaa tcaacggcgg cgtgctgacc
     7681 attacctttg aaaacggcag caaaatcatt atcaaccgcc aggaaccgct gcaccaggta
     7741 tggctggcaa ccaaacaagg cggctaccat tttgatctga aaggcgatga gtggatttgc
     7801 gatcgcagcg gcgaaacctt ctgggatttg ctggaacagg cggcgacgca gcaggcgggt
     7861 gaaacagtca gtttccgcta agattgcatg ccggataagc ctcgctttcc ggcacgttca
     7921 tcacgaaaaa tattgctgta atagcggcgt atcgtgatcc tgattggcag gcggcatgtt
     7981 accgatagat tttgtgcgga acggaatcac ctgttcacga ccatcaacct tcacaatctg
     8041 atagaactgc ggcaggttga agttgatgaa gcttgagccg taggtgaagc ggtcgtgcga
     8101 tgacgagtag aagcgactga cgtcacgcac cagttcctct ttgctgcctt cgcagtggtg
     8161 atacacctca acccggttgc tttcgtcaag aatgtagata ttaaagccat tctcgtcttg
     8221 cgtttcttcg aagaagaact ggatgatccc ttcactagca aagccgtcca ccaccgccgg
     8281 taatttgacg tgattggttt caacctgcac tgacaggccg tgcagtttgt tatgcgaaat
     8341 agcgccataa aactcgatgg cgttctccag cttctgtacc gatacattca ggcgttcgaa
     8401 gaacaagccc caggtttgac cagaaacgcg cagcgccttg aaacgcccgg tttcctggcg
     8461 ggtgctggaa aggcgcagtt caatacactc agaaaccagt tgctgcacgc gagtacggat
     8521 taacccgcgc agatgctggc tataacagaa gacttccacg ctatctggcg gtgcggcgtc
     8581 ctgatgcatt ttgccgagaa tagttttcag ggcttcgatc atcgattgct cgccgttgaa
     8641 gtgcagcgta cgcacttcgt tccacgagtt gcggtacagc aggtcaacgc tacctaccag
     8701 gcaattttga ttctcgccaa agctgaagac atccagctta cggaaatcaa aatgcaccac
     8761 ctgattgcgg aacgccgctg tcgggtcata ttccaggtta acgataatcg ccagatggcg
     8821 gatctcacac gggctgtaga gcgccttcgg tgtcggtgca ggtaagcgca gcgggaaatg
     8881 gtgcgacacg tcggcgacca tctcctgcaa cttaggcaaa tcgacaatgc cgttaccttt
     8941 aatatacaaa cgggtgcgcg aggtcagcag accgttaaac catgcccacg ccaccagttt
     9001 attcaggtaa cggttatatt ccagcggctg atggctgatg atcgactcaa tatttggcgc
     9061 gcggttatac agataccaac ctgaacggtt agcccggccc ggcggcacat aaataaaggt
     9121 cagattcggt tccgagagat cgggtgaaat ctgcgggttt accagcgtca ctttacctgg
     9181 taatgcttca aacgcggcat acagcttacg cgtcagcacg ccgatatcct gcggactggc
     9241 ggagacgcta aggttattgc gacgcgcaaa gcggatcaga ttacggtagc tctgcatcat
     9301 cgcgtcgagc aactcgttgt gcgcctcacg cacctgatca atcttccagt tagcacggtt
     9361 atcgagcatt gccagacgag cttcgtccca accccactcg ctcactaact ggctcaacac
     9421 tgcgcgacgc cagcctacac aggcgcgttc acggctgagt ttttcgcaca cttttaaata
     9481 gaagcagcga cgtactaaat ccagacgggt gaaatcttca atcgccgtca ggtattcagt
     9541 aacacgctcc agcatcatgc agtatggatc gaggccaaac gatacaatct cgccgtcgtg
     9601 caaacgctgt ttgatatctt tcgccagcag acgtgggttc gggtattccc aggaataggc
     9661 ttccagcagc agtgttttca gcaccgcttt gtatggggaa tcgatactct tgtagagctg
     9721 ccaaaggctg gcaccaaagt actcttcagc agaaagcgag cttaagccac cgagatccag
     9781 ccattcattt ggcgtcagca cgccctgcgc gtaaagcgtc atcacatagt cgtcgtaatg
     9841 ctcttcttcg tcgcacggca ccatattcca cagaatacgc ttaccggcga gacgcacggc
     9901 ggtacgataa aattcgtcaa gcagcagtat atgctgggtg gagccacaat cttcgccccc
     9961 caggctgccg ctttcattat gacgaaagcg gttttcatca atcaggaaga agctgacttc
    10021 cacacccagc gaggcggccc agctttccag caggctgcat ttacgttgta gtaactggcg
    10081 ctcttcgcta tcgagccagg attgatgaca gacccagata tccaggtcag aggaacaact
    10141 ttgccctacg gacgaggtgc tgcccatggt gtatacacca gtaattggaa gctcaccttt
    10201 cggtggatcc tgtactgaca ttccacgata cagttcaagc tcgttcaggt agtggcgttg
    10261 agtttcatca ggcgtgtaaa ggcaaatgcc tttgggaacg ttaccatcaa ggtaacccgg
    10321 cattagcgga tggtgatagt gcaacaatgt cggcagtaga ctgtagacct gttggaatgc
    10381 aggccccata gcagcaagcg cgcgatccac acgcaattga tttatggcat ccagtctctg
    10441 tttcagagtc tcaatataga ggtacaagac gtatcgcctg atttgctacc cgtcatgact
    10501 gtgattccgc caacatcaac ggtaacacgc ggcattcggg atatttcgta tgtcaaaggt
    10561 aaccgttacc acttttcgcg cctggttttt ttagtttcac gacgaaaaaa tggtctaaaa
    10621 cgtgatcaat ttaacacctt gctgattgac cgtaaagaaa gatgcgctac atacaagtgt
    10681 agcaccgttt attctctgta aattccttat tacgacagcg tgaaacgtct gtcaggatcc
    10741 actgccagac ctcattttac ggtttgcgca ggcgtctacg tttcaccaca acactgacat
    10801 cactctggca aggatgttag gatggaccac ggatgataat gacggtaaca agcatgttag
    10861 acaatgtttt aagaattgcc acacgccaaa gcccacttgc actctggcag gcacactatg
    10921 tcaaagacaa gttgatggcg agccatccgg gcctggtcgt tgaactggta ccgatggtga
    10981 cgcgcggcga tgtgattctt gatacgccgc tggcgaaagt aggcggaaaa ggcttatttg
    11041 taaaagagct ggaagtcgcg ctcctcgaaa atcgcgccga tatcgccgta cattcaatga
    11101 aagatgtgcc ggttgaattc ccgcaaggtc tgggactggt cactatttgt gagcgtgaag
    11161 atcctcgcga tgcctttgtg tccaataact atgacagtct ggatgcgtta ccagcaggca
    11221 gtatcgtcgg gacgtccagt ttacgtcgcc agtgccaact ggctgaacgc cgcccggatc
    11281 tgattatccg ctccctgcgc ggcaacgtcg gcactcgcct gagcaaacta gataacggcg
    11341 aatacgatgc catcattctt gccgtagccg gactaaaacg tttaggtctg gagtcccgca
    11401 ttcgcgccgc gttaccaccc gagatttctc ttccggcggt aggacaaggt gcagtcggta
    11461 ttgaatgccg ccttgatgat acgcgcactc gcgagctgct tgccgcgctg aatcaccacg
    11521 aaactgcact gcgcgttacc gcagaacgcg ccatgaatac ccgtctcgaa ggcggatgtc
    11581 aggtgccaat tggtagctac gccgagctta ttgatggcga aatctggctg cgtgcgctgg
    11641 tcggcgcgcc ggacggttcg cagattattc gcggtgaacg ccgcggtgcg ccgcaaaatg
    11701 ccaaacaaat ggggatttcg ctggcagaag agctactgaa taacggcgcg cgcgagatcc
    11761 tcgctgaagt ctataacgga gacgccccgg catgagtatc cttgtcaccc gcccgtctcc
    11821 cgctggagaa gagttagtga gccgtctgcg cacactgggg caggtggcct ggcattttcc
    11881 gctgattgag ttttctccgg gtcaacaatt accgcaactt gctgatcaac tggcagcgct
    11941 gggggagagc gatctgttgt ttgccctctc gcaacacgcg gttgcttttg cccaatcaca
    12001 gctacatcag caagatcgta aatggccccg actacctaat tatttcgcca ttggacgcac
    12061 caccgcactg gcactacata ccgtaagcgg acagaagatt ctctacccgc aggatcggga
    12121 aatcagcgaa gtcttgctac aattacctga attacaaaat attgcgggca aacgtgcgct
    12181 gatattacgt ggcaatggcg gtcgtgagct aattggggat accctgacgg cgcgcggtgc
    12241 tgaggtcact ttttgtgaat gttatcaacg atgcgcaatc cattacgatg gtgcagaaga
    12301 agcgatgcgc tggcaatccc gcgaggtgac gacggtcgtt gttaccagcg gtgaaatgtt
    12361 gcagcaactc tggtcgctga tcccacaatg gtatcgtgag cactggttac tacactgtcg
    12421 actattggtc gtcagtgagc gtttggcgaa actcgcccgg gaactgggct ggcaagacat
    12481 taaggtcgcc gataacgctg ccaacgatgc gcttttacgg gcattacaat aactctcata
    12541 acaggaagcc ataatgacgg aacaagaaaa aacctccgcc gtggttgaag agactaggga
    12601 ggccgtggac accacgtcac aacctgtcgc aacagaaaaa aagagtaaga acaataccgc
    12661 attgattctc agcgcggcgg ctatcgctat tgctctggcg gcgggcgtcg gtttgtatgg
    12721 ctggggtaaa caacaggccg tcaatcagac cgccaccagc gatgccctgg ctaaccaact
    12781 gacggcattg caaaaagccc aggagagcca aaaagccgaa ctggaaggca ttattaagca
    12841 acaggctacg cagcttgaac aggcgaatcg tcagcaagaa acgctggcaa aacagctgga
    12901 tgaagtccag caaaaggtcg ccaccatttc cggcagcgat gctaaaacct ggctgctggc
    12961 ccaggccgat tttctggtga aactcgccgg acggaagctg tggagcgatc aggacgtcac
    13021 gaccgctgca gcgttgctga aaagtgcaga cgccagcctg gcggatatga atgacccgag
    13081 tctgattacc gttcgtcggg caattaccga tgatatcgcc agcctttctg cagtatcgca
    13141 ggtggattat gacggcatca tccttaagct taatcagctt tcaaatcagg tagataacct
    13201 gcgtctggcc gataatgaca gcgatggttc gccgatggat tcagacggtg aagagctttc
    13261 cagttccatc agcgaatggc gtatcaatct gcaaaaaagc tggcagaact ttatggacaa
    13321 cttcattacg attcgccgtc gtgatgacac cgccgtaccg ctgttagcgc caaatcagga
    13381 tatctatctg cgcgaaaata ttcgctctcg cctgctggtc gcagcacaag ctgtaccgcg
    13441 tcatcaggaa gagacttatc gccaggcgct ggagaacgtc tccacctggg tacgtgctta
    13501 ctacgatact gatgatgcca ccaccaaagc gttcctcgac gaggtggacc agttaagcca
    13561 gcaaaatatc tcgatggatc ttccggaaac cctgcaaagc caggcgatgc tggaaaaact
    13621 gatgcagacc cgcgtgcgta acctgctggc acaaccggca gcggggacaa cggaagctaa
    13681 acctgcacct gcacctgcac ctgcacctgc accgcaaggt gatactccgg cagccgcgcc
    13741 gcaaggagaa taaccatgct aaaagtgtta ttactctttg ttttgctgat tgcggggata
    13801 gtggttggcc cgatgattgc cggtcatcag ggttacgtgc tgatccagac tgacaactac
    13861 aatatcgaaa ccagcgtcac gggcctggcg attattttga ttctggcgat ggtagtgctg
    13921 tttgccattg agtggctact gcggcggatc ttccgcacag gcgcacacac ccgtgggtgg
    13981 tttgtcggac gtaagcgtcg ccgtgcccgt aagcagaccg aacaggcgct gctgaaactg
    14041 gcggaaggcg attatcagca agttgaaaag ctgatggcga aaaatgccga tcacgcggaa
    14101 caaccggtgg tgaactatct actggctgcc gaagccgcgc aacaacgtgg tgatgaagca
    14161 cgcgccaacc aacatctgga acgcgcagca gagctggccg gcaatgacac cattccggta
    14221 gaaatcaccc gtgtacgtct gcaactggcc cgtaatgaaa accatgctgc acgccacggc
    14281 gtggataagc tgctggaagt tacgccacgc catccggaag tattacgtct ggcggaacag
    14341 gcgtatatcc gcacaggtgc atggagttct ctgctggata tcatcccatc aatggcgaaa
    14401 gcccatgttg gtgatgaaga acatcgtgca atgctggaac aacaggcatg gattggcctg
    14461 atggatcagg cgcgtgccga taacggtagt gaaggtttgc gtaactggtg gaaaaaccaa
    14521 agccggaaaa cgcgtcatca ggtagcgttg caggtggcaa tggcggaaca tcttattgaa
    14581 tgtgacgatc atgatactgc ccagcaaatt atcatcgatg gcctgaaacg ccagtacgac
    14641 gatcgcctac tgctgccgat tcctcgactg aaaaccaaca atccggaaca gctggaaaaa
    14701 gtgctgcgcc agcagatcaa aaacgtcggc gatcgcccgc tgttgtggag cacactgggc
    14761 cagtcactga tgaagcacgg agaatggcag gaagcatcgc tcgccttccg cgcagcgctg
    14821 aaacaacgtc cggacgccta cgattacgca tggcttgccg acgcgctgga cagactgcac
    14881 aagccggaag aagctgcagc gatgcgtcgc gacggtttga tgttaacgtt gcagaacaac
    14941 ccaccacagt agttccttct cacccggagg caagcacctc cgggaccttt ctgatgcata
    15001 aaaaaacgcc tgctcttatt acggagcagg cgttaaaaca ggtctgtatg acaacagtgg
    15061 gtgcttcact caacgttgtg tccatggtgt ctgatgaggc ataagcgaca tctgtcagtg
    15121 gacgataagc accgtaaacg gctctgcgtc attccggagt ttatgaggca ctaaggcgaa
    15181 cataagagat ggaatgagca tctacccgtt tattatgcca cagagaatcg ggaaataaca
    15241 tcccttaaca cttgttatga gataattctg taatcctctt tgcttcctga gtgaatattt
    15301 aacctgagct tgatcctaca catacttatt atgaatgata aaattcattc aattaataac
    15361 acatatatta attgccgtta aaactaaaaa agcattaata atcaacgcaa tataataaac
    15421 ccgccttaca tatcaactgc gccagaggta ggattgaaaa cgctctcctg attttccaat
    15481 tcattttctg gataaataaa taatttattt ttgtcactat tatttatgta atcatcctgt
    15541 cagggagagg gatctcaatt atcaatgctt aattacgtca tcattttgat gacatgaaac
    15601 cggagcattt tgcatggaat tttcgttttc acccaaactt cttgttgttg ctgtcgccgc
    15661 cgctcttcct ctcatggcca gcgcagcaga taccccgtca actgccaccg cacgcaaagg
    15721 ctttgccgga tacgatcacc caaaccagta tccacagaac gctgcaattt attgaatttg
    15781 cacgatcatg taggccggat aaggcgttta cgccgcatcc ggcaatcaac cgcaggcggc
    15841 cacaaaatgg ccgctttttt acttattcac caccagcaaa tgtgcctgca taatgtcact
    15901 ggccgggcga ccgtgcgcca gcaaatcagc cattgcttta agatatggcg gtagatggcg
    15961 gaaataacgc tgatacccgg cacacaaata attcagcccc ggtttgccgc tggcatcgag
    16021 catgaagcgg tgtttcgggc agcctcccca gcacgctttt aacacgttac aactgcgaca
    16081 ctgcgccggt aattgcttaa atttatcttc accaaacacc tgctgttgcg gggaatcgat
    16141 catttctgca attgtttgct gatgcatatt ccccagccga tattgcggat aaacatagtg
    16201 atcgcaggcg taaacgtcgc cgttgtgctc aacaatcacc gagcgcccac aggttggctg
    16261 atgatggcaa accgcacccg gcgcaccgac aaaattggca aacgcccatt cgatattcat
    16321 cacgaaaatc ttgccgacgt cgcgtttgat ccagtggtcg aatatcgcca ccagaaattc
    16381 accaaattca tcggggcgca ccgaccattc cgtcagttcc ccctgaatat caccaggcgc
    16441 atgcaatttc agtccatcgc gggcagctgt ttcatcagcc agacgctcga ccaccggaat
    16501 aaactggatg aattcgactc ccgcatcgca caaaaaatca taaacctgca acggttgctg
    16561 cgcgctggtg cggttgacgc agaccagcac gttatagtcg acatgatgtt tttgcagcag
    16621 cgtcagggca cgcatcacca gcttatgcgt tggtctgcca cctttggtca cgcgatattg
    16681 attgtggatc tcagccggac cgtccagcga taacccaaca agaaaatgat tttctgccag
    16741 aaatgcacac catttatcat cgagcagcac gccgttagtc tggaagctgt tacttatctt
    16801 cctgccagca ccgtatttcg cctgtaactt tacggcacgg cggtaaaaat ccagcccgag
    16861 taacgttggt tcgccgccct gccaggtaaa agccacttcg ttttgcggtt cgctggcagc
    16921 gatatagtga cggacatacg cttccagcgt gtcatcgtcc atatgcgtga ctggcttttc
    16981 gcggtaaagg gattgttttt cgagataaaa acagtagtca cagttcagat tacaatcgga
    17041 accactcggt ttcgccatca catgaaaagc acgcgttgga acctgttgca gcatggtcac
    17101 tccttgcctg aagtcatcct tcaatacgtt aagggcgaaa tcataccgtg gggcaatgag
    17161 ttattacggg cgtttattgc gaagggaggg tgacgaaatg cacagaaaac aaaaaacccc
    17221 gccgaagcgg ggttcaaaat tggtcggcga gagaggattc gaacctccga cccactggtc
    17281 ccaaaccagt tgcgctacca agctgcgcta ctcgccgaaa tactgctttt tgaattttta
    17341 gttcaattct ttaaagtcgt ggtgcgaggg gggggacttg aacccccacg tccgtaagga
    17401 cactaacacc tgaagctagc gcgtctacca attccgccac cttcgcattg tcacaacttc
    17461 taataatggg gtggctaatg ggattcgaac ccacgacaac tggaatcaca atccagggct
    17521 ctaccaactg agctatagcc accactacaa atcttgttac gcggtattac taccaccgca
    17581 gctcaagcgc cgggactaaa tggcgcgccc gacaggattc gaacctgaga cctctgcctc
    17641 cggagggcag cgctctatcc agctgagcta cgggcgctta gcgccgttgc gggggtggat
    17701 aatacggact tcccacccct ctgtctagtc cctttttaaa taaattgcgc gtttggttac
    17761 ggtttgtgcg ttttgctgct tattcctcca gtttatgcgc tttcccgtgg cgattaaggc
    17821 caaaaacttt ataaatcgcc gtcaccgcca gcataaagat gatgccaaca aacagcgaca
    17881 tacgcgtatc ttcattaaag tacatgccga tcaaaacgca aatcaggaat gccattgtta
    17941 cgtagttggc ccacgggaac aggattgagc ggaacggatg gctggcaatc gccgctttat
    18001 gtgcacgccg aaaacgcagc tggcttatca atatcacaaa ccatggcacc atccccggaa
    18061 gcacgctggc actgtagacg tagacaaaca cacgctgcgg attgggaatg atgtagttca
    18121 ggcatgagcc aattagcaga atagcaatag atactgccac acccgcaact ggtacgccgt
    18181 gacgggaaac tttcgccatc gccgccggta actgacggtt tttcgccagt gcgtagagca
    18241 tacgtccgca actgtacatg ccgctgttac agccagagag cgcagccgtc agcaccacaa
    18301 agttgataat gcccgccgct gcggtaatac cgattttggc aaaagtcagt acgaacgggc
    18361 tgccgttgct gccaatttca ttccacggga agatggtgac gataacgaaa atcgcgccta
    18421 cgtagaaaat caggatccgc cacagcactt tgcctacagc actgcgcagc gtcacctgcg
    18481 gattcttcgc ttcaccggca gtaatgccaa tcagctccac gccctggtag gacgccacca
    18541 caatacacag cgcggtcagg aaccctttcc agccacccgc aaagaaaccg ccatgctctg
    18601 tgagattgct aaaaccaatc gactgcccgc cattgccaaa gccaaagaaa atcacgccca
    18661 ggccaatgac aatcatcacg ataatcgtgg tgactttgat catcgcgaac cagaactcga
    18721 tttcgccgta caaccgcacc gccgccagat tcgccaacgc caccagcgcc accgcgatca
    18781 atgcaggtat ccactgcgcc atctccggga accagaactg gacataaacg ccaatggcgg
    18841 tgatttctga gatccccacc gccatccaca taaaccagta agaccaggcg gtgagataac
    18901 caaaaaacgg gctcatataa cgatgcgcat aaacggcgaa cgaaccggta acaggttcaa
    18961 ggaacaacat ttcgcccatt gaacgcatga tgaaaaagac gaacagcccg gcgatgatat
    19021 aggccaacaa tacggatggc ccggcccatt tcagggtact ggcggccccc ataaacaggc
    19081 cgacgccaat agtgcccccc agggcgatga gttcgatatg tcgagcttcc agcccacgct
    19141 gtagctctgg tttgttatct gccataaatc ctcgtgttgt gtttgcatgc tttccggtgt
    19201 taccggttat cgttatgggt acatcgagtg ttgcgaatgt tttcgtaatt caggagaaat
    19261 ggcaaataaa gcattaaaaa tttgaatgct ttgtgtaata aaaaagcaga caggcgacgg
    19321 agtgaccact ccgtcgcttt acaaagagag gaaaatcaga gattgccggt gtaatgccag
    19381 cgtaaataac gcagcaaacg aagctgacgc ttaatgcggc tcggctgcga aagcaggcgg
    19441 tagagccact ccagtcccag cgtttgccag attttcggtg cgcgttttac gtgaccggtg
    19501 aaaacatcgt aagtcccgcc aacgcccata tacagcgcat ctggatgcac cagacggcag
    19561 tcgcgcatga agatctcctg ctttggcgat cccatcgcaa cggtgacgat ttgcgctccg
    19621 ctggcatgaa tgcgttcaaa cagcgcctga cgctgctcgg gtttaaaata accatcctga
    19681 ctgccaacga tattcacatt ccattggttg cgcagtttag cttcagtttg cgccagcact
    19741 tcaggtttac cgcccacaag aaataccggc gtcccttctt tgcctgcgcg cgccatcagc
    19801 tcttcccaga gatcggcacc ggcaacgcgg gaaacctgcg cctgcgggta ctttttacgt
    19861 actgaacgta caacgctgat gccatccgca tatttaaatt cggcagcgtt aattaactcc
    19921 ctgacctcgg cgttatcttc aatggtcagc attttttcag cattaatggc aaccagcgtt
    19981 ccctgcttaa gctgcccgtc agcaaacaga taatcgaggg cgtgctgcat atcacgccaa
    20041 ccaatcaact gtaagccacg cagcgtatag gtcggtgccg tggtgttgtt attcattgtt
    20101 atccttcaac ctgcgtccgg agcgatgatt ttgtacgttt atgaatgagt ccggcgcttt
    20161 caaaaagcca gtacagcagt tttgcgatca tcagacatgc gccgaagacc acgataaaaa
    20221 agactacgcg tgagacaaac gaatccagcc cttcacgcgc cagcacgatc atattgaaga
    20281 tcgccccaaa gcagaaactg tgcaatatcg cagccttata gcgattaggc tcgcggttgc
    20341 ccagctcata cagccagtcg aaccatttga tgatcagacc aaccacgatc gccccgagcg
    20401 ggatgaacaa cgcgccgccc atcaccacca gtgagcctat aagcgtaggc gagatcgcca
    20461 gtccggagtg gttattcagc acttcccagg taaagtagtt ggctgagttc agcaccatac
    20521 tcgggcgacc cggccacagc caggaaggga taaagacata gaaatcgcgg acaattggag
    20581 ccaggccctg gaagtcgatg ttgtcgtagt tctgcaacag caacgccaga ttctcccacg
    20641 gggagaaggt gtcgcgagtg agatagagaa acgtatagaa cgcttcatcg ccgctcacat
    20701 tcattccata gcgttttagc gccagccaga acatgccaac aatccccagc acgcccgccg
    20761 ccgccagcat ccacaacgaa atccagccgc gaataatgcc aataaacagg aagatagcga
    20821 atgcgatgat gatattggcg cgagtgccgc cgacaatcat ataagtcagc aagccaaagg
    20881 cgaccgtgct gacgaggaaa aacagccacg ctttgctgtc ctggcgcaga aagtagacca
    20941 ccagcatcgc cgggatgaaa aagtaaaaga agcgttttaa cgccacgccg gagacttcac
    21001 tggagaagat ctgactgtag gagttaagcc ggaacagcaa aaagccgttg tgcatgaaga
    21061 agatgccgac gcttaccagc gcgataccca tcaggatcac ccacgtaaga ttggtctcca
    21121 cgcggttcat ggtaaacagc ggacggcgcg gtacatcagc aacgcgtttg cgtaggcggg
    21181 ttttgtaggt gacatagtaa accgcgtaga agcagcccgc agaaagcaac gcctgcaaca
    21241 agatttctgg cggcgcgaca ccaacatcaa agcgaaatac cagcacgctg gtcagcggga
    21301 agccgaagaa aaaggtgagc aaaaacagca atgaaaagaa gacattgaag ttaaagcgca
    21361 cacggcgaaa ctcaaaccag gtcagcgtgg caataaacag cgtgcagagc agccagacca
    21421 caaacaggcc actgaattgc agcagactca tgcgacctcc ccggcggcaa tcgccaacgc
    21481 ccgctgccag ccttgtagat agttagggct aaagaaggca atggtgtttt tatccaccga
    21541 cgccaactgg cgctgcgctt cacgcacaat atcctcgttg agatcgtcgg tagtaaacag
    21601 caccggcaaa tgctgttccg tcatatcctg ccagaacgga ttttcccggt taagcacaca
    21661 aggaatgccc gcctgaatca gtaagcaaag cgtaccaatg ccctgctggc gggcaaaaat
    21721 aaagtaacca agatcgcact gacgaagtag cgccagatag gcgtcaaatt ccagtttttc
    21781 gctcaaaatt tgcagatttt cttcgctgaa taactccagc cccgcctgac gaacttcctc
    21841 aatgtacgct tcgttattag gcggatatcc catcggcacc accactttta ccgtatcgcc
    21901 aaattgctga tgaacggcgc gcaaggcagc aatatggtca ttgctgcggt caccggagtt
    21961 ccccaccaga atggtcattt tcccttcacg ttgccgatca ttcgccatcg tattgagaga
    22021 agggtccatc cgcgtcggga agtacagcag ttcgccccgc acctttggat gcgttttggc
    22081 aaaaaagctc aaatcaccgc gggtggcaaa tacacagccg actcgctttt gcgccaggcg
    22141 acgtagcggg taaaaaagct tatatctcaa gccactggaa agctcataca ggtctgcccc
    22201 ccagatatgc cagaaaaact ggctgggctt aatgccacca ctcagcagag ccagccacag
    22261 tgtgggattg aactgaccgt ggaaaaaaaa tcgctgctga cggttagctt ttgctttcgc
    22321 gatgaccgct tccgccagcg attttttccc agggaaaaat tgcacagaaa gcgccggaca
    22381 gctatcactt aagccgtcgt ccttgccaac aaccataaac tcgcgcgcgt gctcgctcgt
    22441 cgcggccagc gcgtcattga aaaaccgcaa aacggttcgg ttatggtgag ggatatccga
    22501 tcccagtacg tgaatcagta cagtcatgcc cgcctacgcc agagtaaaaa cacgccacaa
    22561 caaagagaaa aatagacgat ataagttgcc atatatgcct gcgccgcgcc cagtgcacca
    22621 tgcgcaggga ttagccagtg ggcaaatacc atcaataaag tgaactggct gacttccgcc
    22681 agaatataaa accgcagtga cgctttggcg atcaccagat aaccaaagac ataagcgccc
    22741 acttttaaca catcacccac taactgccag gcaaagagat cgcgcatagc ggtaaattta
    22801 ttcgacaaca gcaaccagat agcaaaatca cgcagcaacc agacggtaaa actcgccgcc
    22861 gccactgccg gtaagacgaa tttcagcgat ttaaccactt cccgggtgat atcgcgcttt
    22921 tccgttagcc gcgacaacgt gggcagcaaa tatacgctga acgatgccgt aataaattgc
    22981 aggtaggcat cggaaatact gctcacccct tgccagatcc ccacttcatc ccagctatac
    23041 tgcgccgcca gcagtttacg catcatgatg taagcaacag gcaaggtcac cgacgtaatc
    23101 aacgccatga gcgtaaattt gctcaactgc cctgccagac cgttatccca gctgggtttc
    23161 agatagctta acgggatgac accacgtttg atcaacatga tggcggcagg aatcaccacc
    23221 agcgcgggaa tcagcgccag acccagcaac gccccttcat aaccgcccaa acggtaactg
    23281 acgtagtacg cgagaacgcc aatcaagctg ccgacaatca gcgataacgc attacctgcg
    23341 gcatcgcgaa agcctttcat cagcgccagt aacaggttgc cccaggcgat ccccatttgc
    23401 accagcgcca ctaaacgcac cagcccctga tagtcggtat taccaaacag tccctggctg
    23461 attggcgcag ctgccagcac aaaaaccagc gccatcagcg tagagaaacc aagtaccatc
    23521 gctgatgaag tgccgaccac gcggcgcagc tgttgcggat tatcatggta ctgggcaacg
    23581 tatttggtta caccgttaaa gatgccagct ccggcaagca cgccgagcac ggtaatcaac
    23641 tggcggaaat ttgccgccag cccaagcccc gccggaccaa atgacacagc cagcaactta
    23701 ccgaccagta acccggcacc aatcttgacc agtgtactgg ccgccgtcca caaggacgct
    23761 tttgccaacg acatatcagg agaagtagtt caacaaagtc gcaattaccg tacgctgatt
    23821 gacgggcgac aggttgtaga acagcggcag gcgcagcagg cgctcgctct ctttggtggt
    23881 gtagcgatct tcaccgtgga actcaccaaa gcgttccccc gcagggcaac cgtgcagcgg
    23941 aatgtaatgg aacaccgcca tgatttccgc ttctttcaga aagttaatca acgcgctccg
    24001 gtcatcaata tcccgcagtt taatgtagaa catatgcgcg ttctgcacgc agccatcggg
    24061 aatcgacggc agctcgatac gcccggcttt cgccagaggc gctaacgcat cgtagtagtt
    24121 ttgccacagc gccagacgtt gctggttgat acgatccgct gcttccagtt gcgcccacag
    24181 gtacgcagct tgcagatcgg acatcaaata gctggagcca atatcgcgcc aggtatattt
    24241 atcgacctga ccacggaaga actggctgcg gttagtgccc ttttcacgga tgatctcggc
    24301 tcgttcgatt aacgctttat cgttaatcag cgtcgcgccg ccttcaccac ccgccgtata
    24361 gtttttggtt tcatggaagc taaagcagcc aatatgacca atggttccca gtgcacgccc
    24421 tttgtaagtg gacatcacgc cctgagcggc atcttctacc acaaacagat tatgcttttt
    24481 cgccaacgcc ataatggtgt ccatttcgca ggccacaccc gcgtaatgga ccggcacgat
    24541 aacgcgcgtt ttgtcggtga tcgccgcttc aatcagcgtt tcgtcgatgt tcatggtgtc
    24601 cgggcgaaca tccacaaaaa cgatttttgc gccacgcagc acaaaggcat tggcggtgga
    24661 gacaaaggtg tagctcggca tgatcacttc atcgccaggc tggatatcga gcagcagcgc
    24721 cgccatctcc agcgaagcgg tgcaggacgg cgtcagtaac actttggcgc tgccaaaacg
    24781 ttgctccagc cactgctggc agcgacgggt aaaaccgcca tcgccacaca gtttgccgct
    24841 acccattgcc gactgcatat agtcgagttc ggttcccacc accggcggtg cgttaaatgg
    24901 aatcatgtga tcacctgtat aaccagtacg cggtgctttc tacattcgca ccactttgta
    24961 tgtatcgttt aagcgcggcg gtgttgccca tttgggtcgc cacccgcaaa gttgttttac
    25021 cgcgagcata cgcccagttt agcgccgttt gcatcagctc agcacctgca ccgcgtccag
    25081 ccagcaggcc aattcgcgca tctgtcgcat tgagttcccg taaagagaca taaccgcgaa
    25141 taccgccgga cgccgcacgt aaaatcagac attgatgatc aaaggtgccg cgcacggcat
    25201 tttcaatcca ctgtgcataa aagcgtccgc tggcgtcagg cgcataccac ggcgcacgaa
    25261 aacggctttg cgcaaatgcg gcgctggcta actgacgtaa tgcgggaata tcggtctctt
    25321 gtgccactac agcaccacta tcactgacat tgttcacggg tagtgccaaa tcaacttcac
    25381 cttctaccag ggagaatccc agctgttgca gggcatccag ttcacccgta tttgatgccg
    25441 caattttggc ctgcacccgt gaccacggcg ctaacatgtc tggcgtcagg agcggtgctt
    25501 cagacgtaat gcgcacgatg gcgctgttaa caccaaagaa ggcgttttcc caggttagtg
    25561 gctcaatact ggcgcggacg ggcacgaagt aactccagca gatattggcc gtagccagtt
    25621 ttcgctaatg aactggcagc acgcttcaca ccctcgtcat cgagccagcc gttacgccag
    25681 gcgatctctt ccaggcaggc aatcttaaag ccctggcgtt tttctactgt ctgcacaaag
    25741 gtactggctt caatcaggct gtcgtgagtg ccagtgtcca gccaggcaaa tccgcgcccg
    25801 agcagttcaa cggtcaggtt gcccgcctcg aggtacatct ggttgatgga ggtaatctcc
    25861 agttcaccac gctccgacgg cttcacctgc tttgcgtact ccacgacttt actgtcgtag
    25921 aaataaagcc cggtcaccgc ccagtttgac ttcggctgtt ttggcttttc ttccagcgag
    25981 atagcgcgga aattgtcgtc aaactccacc acgccaaagc gttccgggtc catcacctga
    26041 tagccaaata ccgtcgcccc ttccgtgcgc gccgcaacat gacgcagctt cggactgaag
    26101 ccctgaccga agaagatgtt atcgcccagc accagacaag aaggttcacc gttgaggaag
    26161 gtttcaccga tgataaaggc ctgcgccaga ccgtccgggc tgggctgttc ggcgtattcc
    26221 agctggatac cgaactcact accatcgccc agcaggcgct ggaaataacc tttatcttcc
    26281 ggcgtagtga tgatgagaat ttcgcggata tcggccaaca tcagcaccga cagcggatag
    26341 taaatcattg gcttatcgta aatcggcaac agttgcttcg atacgccgcg cgtaatcgga
    26401 tgcaatcggg tgccggaacc gcccgccagg ataatacctt tcatgtttgc ctccgctgaa
    26461 aattagcctt tcagacctaa acgctcgccc tgatagctgc cgtcctgcac ctgcttccac
    26521 cagctttcat tagccagata ccactgcacc gttttacgca ttccactttc aaaggtttcc
    26581 tgtggcagcc agccaagttc acgggcaatt ttcgaagcat caatggcata acgcagatca
    26641 tgccccggac ggtcagcgac aaaggtgatc aagtcacgat agtgcaccac gccgtgcggc
    26701 ttgttcggag ccagttcttc cagcagctcg caaatggttt ccacaacatc gagattctta
    26761 cgctcgttgt gaccaccaat attataggtt tcaccgactt tcccggtggt cgccacgcaa
    26821 tacagcgcgc gggcgtgatc ttccacatac agccagtcac ggatttgctg cccgttgcca
    26881 tataccggca gcggtttacc cgccagcgcg ttgaggatca tcagcgggat cagtttttcc
    26941 ggaaagtggt aaggaccgta gttattcgag cagttggtga taagcgtcgg cagaccgtag
    27001 gtccgcagcc aggcgcgcac cagatggtcg ctgctggctt ttgacgcgga ataagggctg
    27061 ctcggcgcat acggcgtggt ttcggtgaag aaatcatccg tcgagtgcag gtcaccatat
    27121 acttcgtcgg tggagatatg atgaaaacgg aacgctgatt ttttatcttc cgtcagcgta
    27181 ttccagtaag cccgcgccgc ttcaagcaat gtataagtcc cgacaatgtt ggtttcaata
    27241 aacgctgccg ggccgtcaat agaacggtca acatggcttt cggctgccag atgcatgaca
    27301 cagtctggct gatgctcagt gaatacgcgt gccagttctg cccgatcgca gatatcaact
    27361 ttctcaaagg caaagcgctc gctttgcgcg accggtgcca gcgacatcag gtttccggcg
    27421 taggtcagct tatcgaccac taccaccgcg tcgctcgttt cgttgatgat ataacgcacc
    27481 agcgccgagc caataaaccc ggcaccacct gttatcagaa tttttctcat cagcgccaga
    27541 ctcctttggc atcgacgaca tactgctgat ggacattgtc gccattgata actttaaact
    27601 gactatgatc gaccagcatc accagcacgt ctgccgttgc cagcgcctcg tcaagctgcg
    27661 ccagagtaca aagcccggtc agttttttcg gcaactggtg gatgttaggc tcaacaacca
    27721 gagtttcgcc gctatgccac tgggcgatca gttcagcgat ttccatcgcc gggctttcgc
    27781 gcaggtcatc aatattcggt ttaaacgcca gaccaaagca ggcgattttc agttcactgg
    27841 cgcgtttatc ggtagccgcc aggcaatcag ccaccgccgc tttcacctga tcgataaccc
    27901 agaacggttt gtgatcgttc acttcgcgcg cggtacggat aagccgcgcc tgctgggggt
    27961 tctgtgccac gataaaccac ggatcaacag caatgcagtg accgcccacg ccagggccag
    28021 gctgaagaat attaacgcga gggtgacgat tcgccaggcg aatcagttcc cagacgttaa
    28081 tcccctgatc ggcacaaatc agcgacaatt cattagcaaa agcgatattc acatcgcgga
    28141 agctgttttc ggtgagctta cacatttccg ccgtccgcga gttagtgacg acacactcac
    28201 cttcgaggaa aattttgtac agttcgctgg cgcgggtcga acaaaccggc gtcataccac
    28261 caatcacgcg atcgttttta atcagctcga ccataacctg gcccggtaac acgcgttccg
    28321 ggcagtaagc aatgttgacg tccgcctgct cgcccacctg ctgtgggaaa gtgagatccg
    28381 gacgcatctc tgctaaccat tctgccatct tctcggttga ccccaccggc gaggtggatt
    28441 caaggatcac cagcgcgcct tttttcagca ctggcgcaat ggagcgagca gccgattcaa
    28501 cgtaggtcat atctggctca tgatcgcctt taaacggcgt gggtacagca atcagccagg
    28561 catccgcttc aactggcgtc gtgctcgctc gtaaaaaacc gccttctacg gcagttttta
    28621 ctacgctcgc caaatcaggc tcgacgatat ggatttcgcc acgattgatg gtatcaaccg
    28681 cgtgttggtt gatatcgaca ccaatcacct gtttttgccg tgaggcaaac gctgctgctg
    28741 taggcagccc gatgtaaccc agtccgataa cagaaatggt cgcaaaactc atagtgatat
    28801 ccgattattt tttaacgctt ccagaatgcg agagcatgcc tgaccatcac catacgggtt
    28861 atgggcgcgg ctcatagttt gatattcgtt ttcgtctttt aaaagacgcg tcacttcctc
    28921 gacaattcgc tgcttatccg tgcctaccag acgcaccgta cccgccgtca ccgcttccgg
    28981 acgctcagtg gtatcgcgca tcaccagcac cggtttcccc agcgaaggcg cttcttcctg
    29041 aatgccgcct gagtcggtca aaatcagcca ggcgtggttc atcagccaga caaacggtaa
    29101 atactcctgg ggatcgatca gaatgacatt tttcacatgc cccagaatgc gattgaccgg
    29161 ctctctgacg ttcgggttga gatgcaccgg atagacaatc tggatgtcct ggtgcgtggt
    29221 ggcaatgtct gccaacgcgt ggcagatttc ttcaaagcca cgaccgaaac tttcacgcct
    29281 gtgaccggtc accagaatca tctttttatc ggggtcgata aacgggtaat ttgccgccag
    29341 ttctgaacgc agcgtatcgc tgctcatcac ctggtcacgc acccataaca gtgcatcaat
    29401 aactgtatta ccagtaatga agattcggct atccgcaacg ttttcacgca gcaagttttg
    29461 ccgggaagtt tcggttggag agaagtgata catcgccaga tgcccggtca atgtacggtt
    29521 agcctcttcc ggccacggcg aataaagatc gcccgtgcgc agaccagcct caacgtgacc
    29581 aacaggaata cgctgataaa acgccgccag gctggttgcc agcgtcgtcg ttgtgtcgcc
    29641 gtgaaccagc acgacgtctg gtttgaactc ggcaagaata ggttttagcc cttccagaat
    29701 ccgacaggtt atctctgtca ggccctgtcc tggctgcatt atgttgagat cgtagtcagg
    29761 tacaatggaa aagagtttca gcacctgatc gagcatctcc cgatgctgcg cagtgacgca
    29821 aactttagcc tcaaaaaaag gatcttttgc caacgcatgc accaacggcg ccatcttgat
    29881 ggcttccggg cgcgtaccaa atacagtcag tactttcaca tcgattctct tcgaataagc
    29941 ggcgagcgcc tttgcgctca ccgcagcagt gttgctattt cgagcaacgg cgggttaatg
    30001 cgacaccagc cccgatcagc cccccgacaa tgccccacat aatcatcagg aaggcacgac
    30061 gtgggctatc gcgttttacc ggttcttccg gcgtacgcaa atagcgatag gtctgaaaac
    30121 gcggatccag ggttggacca acattcaggg tgtttaacat ggcccgattc tgatcatagt
    30181 cgagatcaaa ggccggaccg acggcctgta aattttccag tcgagcctgg agcattggac
    30241 gcccaagcag gaacatttct gaatcaggta attcctcggc aggcacatct gtcgcactgc
    30301 gcgaaatatt atgctgctca gcaattttca gcgcctgttc aatactgttc atccggcggt
    30361 cgtagatggc tttcgccacc tcttcctgac gcttcacctg tgctttcatc tggatggtac
    30421 gcgccgccca tgcgcctttc agctcatcat tcagatggct ggctgcacgc tggctggcaa
    30481 aagcaacata ctgacgtaac aggttattag cgtcaggcgc ggtttcagca ataagcttca
    30541 cgctgtcatt gaccgcgcgg gtaaagtctc cggggataaa ctggatgttg ttaatcattt
    30601 catccagcaa cgccgcatcg gctttgctgt tgcccaccat ccgctgtttg taatagtcgg
    30661 tttgcagcca gaactctctg cgggtatccc acgaggccag ttgcataaca aactctttat
    30721 aggcttcgtc catgaccgat ggttggtcgg cagaagccat gtttgaacgg acatccaggt
    30781 tacgcaaaaa ttgctgctgc gagtaatatc cccccagcat attcaccgtt ggacgatcgg
    30841 taatcgccgt cgagctccac tcctgacgag caaaaaaagt atacgccagc gcgattaacg
    30901 caaatgccag ccccatgcca ataatccata gcttcccagc ccacaaggta cgaaacaacc
    30961 cacgaatatc cagttcattt tcagcgtctt cggccggttt cccaggcatt ggttgtgtca
    31021 tcacatcctc atttatttgg ttaaattggg gctgccacca cgatttctac gcagtctgcg
    31081 ttttacgcgc ttaataaagc gagcaacttt ccaggcacgc ttaatgcaat atccatagag
    31141 gaagaatgct agcaaaaaga gcaccagcat gacccactcc gggacaaaat gagaatattc
    31201 tgccagcacg ccaatggaag cgagcagtgc cgcagcaagg gtaatcagca caaacgcctg
    31261 acgggaagta aacccggcac gcatgatcaa atggtgaata tgctgacggt caggagagaa
    31321 tgggctcatg cctttacgca ggcgacggta cataatcgcc accatatcca ttagcggaat
    31381 ggcgattatc cacaaagcgg taaccgggct gatgggatgg gttttgccct gggtcgtttc
    31441 gagcaggatc cagataacgg taaaaccaat cagcgtactg cccgcatcac ccataaagac
    31501 tttgtagcgg cgccccagga taccaaggtt aagcatgatg tatggcagga tggcggcgat
    31561 catcgcaaag caccagattg cgaggctggt ttgcccgtcg aaccacaaaa tcataccgat
    31621 tgctgcaaac gagacgcagg acaacccgcc cagcaagcca tcaatgccat caaccatgtt
    31681 gaacgcatta atggccgccc agacggcaaa tagcgtcagg aagtaaccaa acggtccgag
    31741 caccatctcc caggagccaa agatataacc caggctactg agataaagtt tgccgaacac
    31801 catcataaca atgccaacag cggcctgtat ggtggcacgg atttttacgc tgatatcaaa
    31861 acggtcatcc agcgcgccaa tgaaaacaag cacaccggca caagcgagat agagagatgc
    31921 atgcggaata tagtaatcga caattccgaa cgtgaagcaa atccctgcgt aaaccgaaat
    31981 ccccccaacg agaggtatca atccctggtg acgtttgcgg aagtttggtt tatccactaa
    32041 accgactttt tttgccacct tacgggcaaa aaacagaaac agtgtcgtga ataaaaaaat
    32101 actgatgaga tcagtactca ctgtcagtaa attcacaatg catgctctca gagaaaatta
    32161 ttagcagaag tataaccacg aagaccttta ttcagaaggg aaagtccgtt ccgaacccac
    32221 cagtcctgtg caaaaattaa ccatttgata tgcaaattgc ataacacgcc agcaaattcc
    32281 aggaattttc ctaacatcgc cagcgcggca ttaagattac agatataaaa gcaaaacgcc
    32341 acgtaaacac gtggcgtttt tggcataaga caaatttatg agcgtttcat catttcgaag
    32401 aaatcgtcat tggtcttggt catggccagt ttattaatga ggaattccat tgcatcgatt
    32461 tcgcccatcg ggtgaatgat tttgcgcagg atccacattt tctgcagttc ttcctgagta
    32521 gtgagcagtt cttctttacg ggtaccggaa cggttgtagt cgatagccgg gaagacgcgt
    32581 ttttcagcga tcttacgaga gaggtgcagt tccatgttgc ctgtaccttt aaactcttcg
    32641 tagataactt cgtccatttt agaaccggta tcgataagcg ccgtcgcgat aatagtcagg
    32701 ctgccgccct cttccacgtt acgcgccgca ccaaagaagc gtttcggacg atgcagggcg
    32761 ttggcatcca caccaccggt caacacttta cctgacgccg gaacaacggt gttgtaagcg
    32821 cgcgccagac gagtgatgga gtcgagcaga atgataacgt ctttcttgtg ctcaaccagg
    32881 cgtttggcct tctcgatcac catttccgca acctgaacgt ggcgagatgc gggttcgtca
    32941 aaggtagaag caacaacttc accttttacc agacgctgca tctcggttac ttcttccgga
    33001 cgttcgtcga tcagcagaac catcagcaca caatccgggt ggttgtaagc aatgctctgg
    33061 gcaatgttct gcagcagcat ggttttaccg gctttcggcg gtgccacaat cagaccacgc
    33121 tgaccacgac cgataggtga tgccagatcc agtacgcgag cagttaaatc ttcagtagaa
    33181 ccgttaccac gttccatacg cagacgagaa tttgcgtgca gcggggttaa gttctcaaag
    33241 aggattttgt tgcgggcgtt ttcaggtttg tcgaagttaa cttcgttaac tttcagcagc
    33301 gcaaaatagc gttcaccttc tttcggcggg cgaatcttac cagagatggt atcaccagtg
    33361 cggaggttga aacggcggat ttggctaggg gaaacgtaga tgtcatcagg accggcgagg
    33421 taggagctgt ctgcggaacg gaggaaacca aatccatcct gcaatatctc cagtacgcca
    33481 tcaccaaaga tatcttcgcc actctttgcg tgctgcttca ggatggcaaa aataatgtcc
    33541 tgcttacgca tgcgagccag gttttccagc cccatatttt cgccgagagt gatcagctca
    33601 gaaaccggcg tattctttaa ttcggtaaga ttcatagtgg tgtgagttct taaacttggg
    33661 gtaaatctcg aacttaatgt tgtgaatggt atggcaggat catccatgcc tgtttacggt
    33721 catcgactca tgtcttttcg ctgcctggtc acaggagagt acgcagaact gaaacgacaa
    33781 gacggattga gtgacgagcc agaaatctgt tcacttcgca tttaagataa aacgggaagc
    33841 gttggaaatt acaagattca aacttaataa ggtatgttta atacgaagtc aacactaagt
    33901 tagcatgact cacgccgggc gtccagtttt tagcgacggg gcacccgaac atgaaattcc
    33961 cttacgccag gttagcgtcg aggaactctt tcaactgacc tttagacagt gcgcccactt
    34021 tggttgccgc cacttcaccg tttttgaaca gcagcagagt cgggatacca cggatgccat
    34081 atttcggcgc agtgccaggg ttttgatcga tgttcagttt tgcaacggtc agtttgccct
    34141 gatattcgtc agcgatttca tccagaatcg gggcgatcat tttgcacgga ccgcaccact
    34201 ctgcccagaa atcgacgagg atcgccccgt ccgctttgag tacatccgtg tcaaaactgt
    34261 cgtcagtcag gtgaataatt ttatcgctca tatataactc cacaggaata agcctggcgt
    34321 gttggtttcg ttgttggtgt aacattaacc aactaaaggt tgactttatt tcaccggata
    34381 cgctttcgta aagcaatagt aagctgatat tctaccacac tatgagcaaa acacatttaa
    34441 cagaacagaa gttttccgac ttcgccctgc atccgaaggt tgtagaagcc cttgaaaaaa
    34501 aagggtttca taactgtacg cccattcagg cactggccct tccgctgacg ctggcgggtc
    34561 gtgacgtagc cgggcaggcg caaaccggta ccgggaaaac gatggcgttt cttacgtcaa
    34621 cgtttcatta tcttctctct catcctgcga ttgccgatcg caaggtgaat caaccgcgtg
    34681 ccttaattat ggcaccgacg cgtgaacttg ccgtgcagat ccatgccgac gcagaaccgc
    34741 tggcggaagc tactggcctg aagctgggtc tggcttacgg tggtgatggc tacgacaaac
    34801 agctgaaagt gctggaaagc ggcgttgaca ttctgattgg caccacgggt cgtttaattg
    34861 actacgccaa gcagaaccac attaacctcg gtgccattca ggtagtggtt ctggatgaag
    34921 ccgatcgcat gtacgatctg ggctttatta aagatatccg ctggctgttc cgccgtatgc
    34981 cgcctgcaaa ccagcgcctc aacatgctgt tctccgccac gctttcgtac cgggtacgtg
    35041 aactggcgtt cgagcagatg aacaatgccg aatatattga agtggaaccg gaacagaaaa
    35101 cgggccaccg tattaaagaa gagcttttct acccttctaa cgaagaaaaa atgcgtttgc
    35161 tgcaaacgct gatcgaagaa gagtggccag acagagcgat tattttcgcc aataccaaac
    35221 accgttgtga agagatctgg ggccacctgg cggcagatgg tcatcgtgtc ggtttgttga
    35281 caggcgatgt cgcgcagaaa aaacgtctgc gtattcttga tgaatttacc cgtggcgatc
    35341 tggatattct ggttgccacc gacgttgccg cgcgtggttt gcatattccg gcagtgacgc
    35401 acgtctttaa ctacgattta cccgatgact gtgaagatta cgttcaccgt attggtcgta
    35461 caggtcgcgc aggcgcaagc ggtcactcta tcagcctggc gtgtgaagag tatgcattga
    35521 atttgcctgc tattgaaacc tatattggtc actcaattcc ggtaagcaaa tacaatccgg
    35581 acgcattgat gaccgatctg ccaaaaccgc tgcgcctcac gcgcccacgt acaggcaatg
    35641 gtccgcgtcg tactggcgct ccgcgtaatc gtcgtcgttc aggttaaata aaatgtcata
    35701 ctcaaaatca ttcaaactgt atcaagatgg caagagaatg aatcccgatg agcatactgt
    35761 agtaagtgat tcaggtgaaa gaatgcagcc aacacagaga cagattgaag gatgaagagt
    35821 atgggttcca cctcgtcgct gtatgcagcc attgatctcg gttcgaatag ttttcatatg
    35881 ctggttgtgc gcgaggtggc tggaagcatc cagacgctga cgcgaataaa acgcaaagtg
    35941 cgtctggctg ctggcctgaa tagcgaaaat gccctgtcta atgaagcaat ggagcgcggt
    36001 tggcaatgtc tgcgcctgtt tgctgaacgt ctgcaagata tccctccctc gcaaattcgc
    36061 gttgtcgcta cggcgacatt acgtcttgcc gtcaatgcgg gtgattttat tgccaatgcg
    36121 caggaaatcc tcggttgtcc ggtacaggtg atcagcggtg aagaggaagc acgtctgatt
    36181 tatcagggcg ttgctcacac aactggcggt gccgatcagc gcctggtggt ggatataggc
    36241 ggtgccagta ctgaactggt aaccggcacg ggtgcacaaa ccacctcgtt gttcagcctg
    36301 tcgatgggct gcgtcacctg gctggaacgc tattttgccg atcgtaatct ggggcaggaa
    36361 aattttgatg ctgcagaaaa agcggcacgc gaagtgttac gtccggttgc cgatgaatta
    36421 cggtatcacg gctggaaagt gtgcgttggt gcttccggca ccgtgcaggc gttacaggaa
    36481 atcatgatgg cacaggggat ggatgaacgc attaccctgg aaaagttgca gcaattgaaa
    36541 cagcgagcca ttcattgcgg tcggctggag gaactggaga ttgacgggct gacgctggaa
    36601 cgtgcgttag tgttcccgag tggtctggcg atcctgatcg ccatttttac cgaactgaat
    36661 attcagtgta tgaccctggc gggcggtgcg ctgcgtgaag gcctggtcta cggcatgttg
    36721 catctggccg tcgagcagga tattcgcagc cgtacgctgc gtaatattca gcgccgcttt
    36781 atgatcgata ttgatcaggc gcagcgcgta gccaaagttg cggctaactt cttcgatcag
    36841 gtggaaaagg aatggcatct tgaagcaata agccgcgatt tgctcatcag cgcctgtcag
    36901 cttcatgaaa tcggcctgag cgttgacttt aaacaagcgc cgcaacacgc tgcttatctg
    36961 gtgcgtaatc tggatcttcc cggttttacc cccgcacaga aaaaattgct ggcgacgcta
    37021 ctgctcaacc agactaatcc ggtcgatctc tcatcgctgc atcagcaaaa tgccgtaccg
    37081 ccgcgcgtcg cagaacaact ctgccgttta ctccgcctgg ccatcatttt tgccagccgt
    37141 cgccgtgacg atctcgtgcc agagatgaca ttacaggcta accatgaact gttgaccttg
    37201 acgcttccgc aaggttggct aacccaacat ccgctgggta aagagattat tgctcaggaa
    37261 agccagtggc agagctatgt ccactggccg ctggaagtgc attaatgagt aagtgccgga
    37321 tgcgatgctg acgcatcttt tccggccttg attatttccc tcgttttgcc gccatcatcg
    37381 ctttcagatt cgccagatgg ctttgccctt tctgcatccg ttcttcggcg ctgaccactt
    37441 tgcgctcctg ttcccaaatc agatcatcct gcggcagctc cagcaaaaag cggctcggct
    37501 ccgggcgcac cagctcgccg tactgacggc gttctttaca cagcgtaaag gtcaattcct
    37561 tctgggcgcg ggtaatgccg acataggcca gccgccgctc ctcatcgata ttatcttcat
    37621 cgatgctgct ctggtgcggc aaaaaccctt cttccatacc gaccatgtag acataaggaa
    37681 actccagccc ttttgacgcg tgcagagtca tcagttgcac ctgatccagc tcttcttcac
    37741 tctcaccacg ctccatcatg tcgcgcaaag taaagcgcgt caccacctgg gtgagcgtca
    37801 tcggctcatc cagttcgctg ccttccagca tctccgtcat ccagctaaac agttggttga
    37861 cgttcttcat gcgcatttcg gcggctttcg ggctgggcga tgtttcgtac agccaggatt
    37921 cataatccat gccgtggatc agatcgcgca ccgcggcaat cggctcccgc tccgccagac
    37981 gctggatttc tgccaaccag tgagtaaagc gggtcaatgc ttcataacca cgtccgctaa
    38041 gcgtctgact caggcccata tcaaagctgg cggtaaacat gcttttattg cgcgtcatcg
    38101 cccactcacc cagctttttc agcgtcgccg ggccaatctc tctcttcggc gtgttaacga
    38161 tacgcagaaa tgcgctgtca tcgtccgggt tagtcagcac gcgcagataa gccagcaagt
    38221 ccttgatttc aggacgagag aaaaacgacg taccaccaga aattttgtac gggatgcggt
    38281 tttgcatcag gaatttttca aacactcgcg actgatggtt accgcgataa agaatagcgt
    38341 aatctttgta ctgcgtttta ttgacgaagt gatgcgcaat cagttcacca gtgacgcgct
    38401 cggcttcatg ttcttcgtta ttcgcgctta aaactttaag ctctgtgccg taacccagtt
    38461 cggagaacag acgcttctca aagacgtgcg ggttattggc gatcaggatg ttcgccgctt
    38521 tcaaaatacg tccggaagag cggtagttct gctcaagctt aatcaccttc agcgccggaa
    38581 aatcctgact cagcagcacc aggttttgtg gacgtgcgcc gcgccaggag tagatcgact
    38641 ggtcgtcatc gcccaccacg gtaaagcgcg cgcggctgcc caccagcagt ttcaccagtt
    38701 catactggct ggtgttggtg tcctgatact catccaccag cagatagcga attttgttct
    38761 gccagcgctc gcggacttct tcattgcgtt gcagcagcaa cgtcggcaat aaaatcagat
    38821 catcgaagtc gagaacattg cacgctttca ggtgtgcatc atacagccca taacaatggg
    38881 caaaaatacg gtcccgctcg ccaatcgcac tggctgccgc ctgggacggt gttttgagat
    38941 cgttcttcca gttagagatg gtcgaaatca actgttgcag gagaactttg tcatcttcaa
    39001 tcagcccttc ggtcaactct ttgagcaacg ccagctgatc ggtatcgtca aacagcgaaa
    39061 agttcgcctt catccccagc gccgcatact cacgcttgat gatatccagc cccagcgtgt
    39121 ggaaggttga gatcatcagc ccacgcgcct ctttgcgacc cagcgtctgt ccgacacgct
    39181 ctttcatctc gcgcgccgcc ttattggtaa aggtcaccgc cgcgatatgt cgcgcctgat
    39241 aaccgcaacc gcggatcaga tgggcgattt tattggtgat cacacgagtt ttaccggaac
    39301 ccgcgcccgc cagcaccagg caggggccgg taacgaattc gacagcttgt tgttggccgg
    39361 ggtttagacg cataggtgta ttgctcaatc ttcgaacggg gggaggattg tagcagaatc
    39421 gcatttgaat gtgaccgcgt gtaagccgct aaccgattgg ctgcggatgt ttaaatcaga
    39481 gagggaagac cgaaaccgga gttcatcgtg aatccattag acttagaaaa tatcgggtcg
    39541 taacgttgtg ttattacttt ttcagagttt actaaggaga ggtgtactcg tggcgattaa
    39601 tttcagtcct aaggttggtg aaatactgga ttgcaacttt ggtaactatc ctgttagcca
    39661 gaatggacag ttcagcacca cgtattacga tggccgcatt cctcctgaaa tgataaaaaa
    39721 caggttagt
//
LOCUS       DAKISA010000033        39409 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.033, whole genome shotgun sequence.
ACCESSION   DAKISA010000033 DAKISA010000000
VERSION     DAKISA010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.033"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..266
                     /locus_tag="OVW45_001741"
     CDS             <1..266
                     /locus_tag="OVW45_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCV5526211.1"
                     /translation="TKAEAPAAQSKAAVPAKASDEEGTRVSINNASAEELARAMNGVG
                     LKKAQAIVSYREEYGPFKTVEDLKQVPGMGNSLVERNLAVLTL"
     gene            360..758
                     /gene="fadM"
                     /locus_tag="OVW45_001742"
     CDS             360..758
                     /gene="fadM"
                     /locus_tag="OVW45_001742"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCV5526212.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(810..1505)
                     /gene="queC"
                     /locus_tag="OVW45_001743"
     CDS             complement(810..1505)
                     /gene="queC"
                     /locus_tag="OVW45_001743"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCV5526213.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(1570..3270)
                     /locus_tag="OVW45_001744"
     CDS             complement(1570..3270)
                     /locus_tag="OVW45_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HCV5526214.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHRETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            3370..4188
                     /gene="cof"
                     /locus_tag="OVW45_001745"
     CDS             3370..4188
                     /gene="cof"
                     /locus_tag="OVW45_001745"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCV5526215.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            4340..4798
                     /gene="decR"
                     /locus_tag="OVW45_001746"
     CDS             4340..4798
                     /gene="decR"
                     /locus_tag="OVW45_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCV5526216.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            4828..6600
                     /locus_tag="OVW45_001747"
     CDS             4828..6600
                     /locus_tag="OVW45_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCV5526217.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDCVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENHEEAVDA"
     gene            6593..8374
                     /locus_tag="OVW45_001748"
     CDS             6593..8374
                     /locus_tag="OVW45_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCV5526218.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSSNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            8555..8893
                     /gene="glnK"
                     /locus_tag="OVW45_001749"
     CDS             8555..8893
                     /gene="glnK"
                     /locus_tag="OVW45_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCV5526219.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            8923..10209
                     /gene="amtB"
                     /locus_tag="OVW45_001750"
     CDS             8923..10209
                     /gene="amtB"
                     /locus_tag="OVW45_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCV5526220.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="OVW45_001751"
     CDS             complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="OVW45_001751"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCV5526221.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            11336..11908
                     /locus_tag="OVW45_001752"
     CDS             11336..11908
                     /locus_tag="OVW45_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCV5526222.1"
                     /translation="MKLVHMASCLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPMQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(11939..12250)
                     /locus_tag="OVW45_001753"
     CDS             complement(11939..12250)
                     /locus_tag="OVW45_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCV5526223.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            12413..12509
                     /gene="ffs"
                     /locus_tag="OVW45_001754"
     ncRNA           12413..12509
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OVW45_001754"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            12629..12982
                     /locus_tag="OVW45_001755"
     CDS             12629..12982
                     /locus_tag="OVW45_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCV5526224.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="OVW45_001756"
                     /pseudo
     CDS             complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="OVW45_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
     gene            complement(14736..15206)
                     /locus_tag="OVW45_001757"
     CDS             complement(14736..15206)
                     /locus_tag="OVW45_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCV5526225.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(15322..15873)
                     /gene="maa"
                     /locus_tag="OVW45_001758"
     CDS             complement(15322..15873)
                     /gene="maa"
                     /locus_tag="OVW45_001758"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HCV5526226.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(16044..16262)
                     /gene="hha"
                     /locus_tag="OVW45_001759"
     CDS             complement(16044..16262)
                     /gene="hha"
                     /locus_tag="OVW45_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCV5526227.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(16288..16662)
                     /gene="tomB"
                     /locus_tag="OVW45_001760"
     CDS             complement(16288..16662)
                     /gene="tomB"
                     /locus_tag="OVW45_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCV5526228.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(17208..20357)
                     /locus_tag="OVW45_001761"
     CDS             complement(17208..20357)
                     /locus_tag="OVW45_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCV5526229.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTSLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(20380..21573)
                     /gene="acrA"
                     /locus_tag="OVW45_001762"
     CDS             complement(20380..21573)
                     /gene="acrA"
                     /locus_tag="OVW45_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCV5526230.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYLDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            21715..22362
                     /gene="acrR"
                     /locus_tag="OVW45_001763"
     CDS             21715..22362
                     /gene="acrR"
                     /locus_tag="OVW45_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCV5526231.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            22490..25852
                     /gene="mscK"
                     /locus_tag="OVW45_001764"
     CDS             22490..25852
                     /gene="mscK"
                     /locus_tag="OVW45_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCV5526232.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(26064..26225)
                     /gene="rsmS"
                     /locus_tag="OVW45_001765"
     CDS             complement(26064..26225)
                     /gene="rsmS"
                     /locus_tag="OVW45_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCV5526233.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(26239..26766)
                     /gene="priC"
                     /locus_tag="OVW45_001766"
     CDS             complement(26239..26766)
                     /gene="priC"
                     /locus_tag="OVW45_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCV5526234.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALCHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            26836..27213
                     /locus_tag="OVW45_001767"
     CDS             26836..27213
                     /locus_tag="OVW45_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCV5526235.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            27366..27917
                     /gene="apt"
                     /locus_tag="OVW45_001768"
     CDS             27366..27917
                     /gene="apt"
                     /locus_tag="OVW45_001768"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCV5526236.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            28046..29971
                     /gene="dnaX"
                     /locus_tag="OVW45_001769"
     CDS             28046..29971
                     /gene="dnaX"
                     /locus_tag="OVW45_001769"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCV5526237.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVVPTAVMTPTQVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSESAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKASAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    29304..29368
                     /gene="dnaX"
                     /locus_tag="OVW45_001769"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            30024..30353
                     /locus_tag="OVW45_001770"
     CDS             30024..30353
                     /locus_tag="OVW45_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCV5526238.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            30353..30958
                     /gene="recR"
                     /locus_tag="OVW45_001771"
     CDS             30353..30958
                     /gene="recR"
                     /locus_tag="OVW45_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCV5526239.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            31068..32942
                     /gene="htpG"
                     /locus_tag="OVW45_001772"
     CDS             31068..32942
                     /gene="htpG"
                     /locus_tag="OVW45_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCV5526240.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            33123..33767
                     /gene="adk"
                     /locus_tag="OVW45_001773"
     CDS             33123..33767
                     /gene="adk"
                     /locus_tag="OVW45_001773"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCV5526241.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKSVAEVRADLEKILG"
     gene            33899..34861
                     /gene="hemH"
                     /locus_tag="OVW45_001774"
     CDS             33899..34861
                     /gene="hemH"
                     /locus_tag="OVW45_001774"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCV5526242.1"
                     /translation="MRQTKIGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(34858..35817)
                     /gene="aes"
                     /locus_tag="OVW45_001775"
     CDS             complement(34858..35817)
                     /gene="aes"
                     /locus_tag="OVW45_001775"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HCV5526243.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            35969..37273
                     /gene="gsk"
                     /locus_tag="OVW45_001776"
     CDS             35969..37273
                     /gene="gsk"
                     /locus_tag="OVW45_001776"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCV5526244.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(37406..39082)
                     /locus_tag="OVW45_001777"
     CDS             complement(37406..39082)
                     /locus_tag="OVW45_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCV5526245.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
BASE COUNT         9525 a        10053 c        10421 g         9410 t
ORIGIN      
        1 aaaccaaagc ggaagctcct gcagcacaaa gtaaagcagc agtaccggcg aaagccagtg
       61 acgaagaagg cacccgggtc agcattaata atgccagcgc ggaagagcta gcccgcgcga
      121 tgaatggcgt tggcctgaag aaagcgcagg cgattgtcag ttatcgcgaa gagtacggtc
      181 cgtttaaaac ggtggaggat ctaaagcagg tgccggggat gggcaattcg ctggtggaac
      241 gtaatctggc ggtattaacc ctgtaattaa tttgcatagt ggcaattttt tgccagactg
      301 aagaggtcat accagttatg acctctgtac ttataacaac aacgtaaggt tattgcgcta
      361 tgcaaacaca aatcaaagtt cgtggatatc atctcgacgt ttaccagcac gtcaacaacg
      421 cccgctacct tgaatttctc gaagaagccc gctgggatgg gttggaaaat agcgacagtt
      481 ttcagtggat gacggcccat aacatcgcct tcgtcgtggt caatatcaat attaactatc
      541 gtcgcccagc ggtattaagc gacctgttaa ctattaccag tcagttgcag caattaaacg
      601 gtaaaagcgg catcttaagc caggtcatta cactggagcc ggaagggcag gtggtagcgg
      661 atgcgcttat tacgtttgtt tgtattgatc ttaaaacgca gaaagcatta gctctggaag
      721 gggaattgcg cgaaaagctg gagcagatgg ttaagtaaac gttttgtggt gccggatgct
      781 caagccgcat ccggcgacac ccggaataat tacctcaacc cggttttctg cttcatcgct
      841 gccatcaccg tcggtttatc ggccagataa tgattcaaac cgttggcgcg taaattacat
      901 gccgcacaat gaccgcaacc gtcgccttta atgccgttat agcaggtcaa cgtttcgtta
      961 cggactaaat ccagtttgcc gtaataatct gccagcgccc aggtttccgc tttatcaatc
     1021 cacatcagcg gcgtttcaaa acgaatatct ttcgccatgc ccaaactgac ggcatggttt
     1081 agtgctttca caaactcatc gcggcaatcc gggtagccgg agaaatccgt ttcacagacg
     1141 ccggtaatta cggcttctgc ttttacctga tacgcatata ttgccgccag cgtcaggaac
     1201 aaaatattac gcccagggac aaacgtattc gggatgccat cggcttcagg ttcataatca
     1261 ggcaccggaa tgctgtcacg tgtcaggctg ctgaccgcca gctcgttgag cagcgttaca
     1321 tcaagaacct tatgcgcgcg tgctcccagt ttcagcgcca gttcgcgtgc cacgtcgatt
     1381 tctgcgcgat gtcgctgacc gtaatcgaac gtcacgcaat ggacttcatc atattgttgt
     1441 aatgcctgca ccagacaggt ggtggaatcc tggcctccac tgaacacaac gacagcacgt
     1501 ttcatagata atcctgcttg aacaataaaa gcgttatggt aacgcctgcg attaacccgg
     1561 accagctatt cattgcgacg gcgcgggaag ccaggcttcg gtaaattcaa accagccgcg
     1621 cggtgtcagt cgcacaccgt tcacgccggg aggggcacta atgcgatagt gatagttgaa
     1681 cagcggcgtc agcgtcgcgt ctgccattaa ctggctaaaa accgctttca gggcattaaa
     1741 tcggttttcc tcatcaggca ttacttgcac cgcgtccagc gtcgattgca gatgtgcata
     1801 tgctggagcg tcgaaaacat gtggccatag tggatcgcaa cgtagccatt gctccagagt
     1861 atattccggt gcttcgccaa ttaatctgtc gcccatcatg aggtctgcgt gtgccagtag
     1921 ggtcgtgtcg tcccagtttt ttgcgttatg aaaaataatt gtgagttcac agccttccgc
     1981 tgccagtgtc gcctgtaggc gttctgccat ggtatgaagt tctatcggta ggtgataaac
     2041 cagcgtcaag gttttcggta gtttgacttc atccggtact tgccagtgcg gaatagtcca
     2101 gcctggcagt aatgcatgac tggcggtgat caggttttct ccgacttcta acgtttgtaa
     2161 taaaccggat tgatgaataa tggagatcac ttttcgcgcc tgccagaggg agagtcgggg
     2221 acttttgcgc aacgttaaat agcaaaaacc taaactgatg ccactactga cctggctgac
     2281 ccgttgcagc tcctccggtt tgccgatggt gatttgcacg ggatgccgac aactggttcc
     2341 caaatctttt tcgaaaagcg gcggagttat ccagtactca accgctttaa gcagcggatg
     2401 acgcaaatgg taataatcat ggctctccag gcgcaccagc tccgctgtga attgtgttaa
     2461 gcgaaaagga ccagtgccga tcagtgggaa ttgcggatgc gccagatggc tgcaatagct
     2521 cgccagccgg tgcgcaagcc agtaatcggg gcgatgtaaa aagaaggtca gacactgcgg
     2581 atgggtgact tcaatacgct tcacgctaat aaataattga tccagtgctg gcagttgtaa
     2641 cagcatcaat aatcgctggt gtaagtgtga ggcttttact gcatcgccgt tatgccagtg
     2701 tagggttgaa cgaagataaa agtcccagcg taacccgtca gtagaggttt ccctgtgatg
     2761 cgctaaatcg ccaatcgggc gctgagtatt attatcgaag cgggtcaggc cggaaaatat
     2821 ctgcccggcg agatgctgct cggcacggcc gggcaaaaag cctggttgta gcggttcgag
     2881 cgggcgatag tagggaatac gcaacgtggg tgtatcgttt tgccattgtc cgcccataaa
     2941 cggctgtaac agagtgcgca gctcacctgg ggccagttgc gccagctcca gcacatcttg
     3001 ctgctttccg gtttccagtg cctgttccat catcgcattg cgtagcgatt ccggcgtgac
     3061 cagaaagcgt aattgtccgc gctttccgcg tcctgactgc gcctgccact ccagccatcc
     3121 cgcctcctgt gcctgacgca acagcgtacg aacatggcgt tcgctgcaaa aacagcgttc
     3181 ggccagttcg ctgacggtga cggtttgcgg ctttccggct gaaggttgcc acagacgttg
     3241 atactggtta agacggttga gcaatcgcat ataaacccgg aacaatatta tttaactatt
     3301 cactattact tccgtatata tcaggtgata ctcaatctcc attaaccgtg tcacagagtg
     3361 gagaaagaaa tggctcgtct ggcagcattt gatatggatg gcactttatt gatgcctgac
     3421 catcatttag gtgagaaaac cctctctact ttggcgcgac tgcgtgaacg cgacattacc
     3481 ctcacttttg ccacggggcg tcatgcgctg gagatgcagc atattctcgg ggcgctatcg
     3541 ctggatgcat atttgattac cggcaacgga acgcgcgtgc attctctgga aggtgaactt
     3601 ttacatcgtg atgatttacc tgcggatgtc gcggagctgg tgctgtatca gcaatgggat
     3661 acccgagcca gcatgcatat cttcaatgac gacggttggt ttaccgggaa agagatccct
     3721 gcgttgttgc aggcatttgt ctatagcggt tttcgttatc agataatcga tgtcaaaaaa
     3781 atgccactcg gcagcgtcac caagatctgc ttctgtggcg atcacgacga tcttacacgc
     3841 ttgcagatcc agctatacga agcattaggc gagcgtgcac atttgtgttt ttccgccacg
     3901 gattgcctcg aagtgctgcc ggtgggctgc aataaaggcg ctgcattgac ggtgctgacc
     3961 caacatttag gtttatcgtt gcgcgattgc atggcctttg gtgatgcgat gaacgatcgc
     4021 gaaatgttag gcagcgtcgg tagcgggttt attatgggca atgcgatgcc gcaactgcgc
     4081 gcggagctcc cgcatttacc ggtgattgga cattgccgaa atcaggctgt ctctcactat
     4141 ttgacgcact ggctggacta tccacatcta ccttattccc ccgaataacg agatcccttc
     4201 cagcaccggg caattgcccg gtttttttgc gttgaatttg tcattttgtg ccgtggtgtt
     4261 taaaccgcac agaataaatt gtcgtgattt cacctttaaa atagaattaa aagagaaaaa
     4321 aattctccgt ggaagggcta tgttagataa aattgaccgt aagctgctgg ccttactgca
     4381 gcaggattgc accctctctt tgcaggcact ggctgaagcc gttaatctga caaccacccc
     4441 ttgctggaag cgcctgaaac ggctggagga cgacggtatc cttatcggca aagtcgccct
     4501 gctggatccg gaaaaaatag gcctcggcct gaccgccttt gtgctgataa aaacgcaaca
     4561 tcacagcagc gaatggtatt gccgctttgt cacggtggtt accgaaatgc cagaagtgct
     4621 ggggttctgg cgcatggctg gtgaatacga ttatctgatg cgcgtccagg ttgccgacat
     4681 gaaacgctac gacgagtttt ataagcgtct ggtaaacagc gtgccggggc tgtcggacgt
     4741 cacttccagc ttcgcgatgg aacagattaa atacaccact tctttaccca tcgaataaat
     4801 atccagaatc aggtcaggac acaacgcgtg cgattatttg ctcaattaag ctggtatttc
     4861 cgtcgggaat ggcgtcgcta tctcggggct gtcgccttgc ttgtcattat cgcgatgctg
     4921 caactggttc cgccaaaagt ggttggtatt gttgtcgatg gcgtgacaga acaacacttt
     4981 actaccgggc tgatcctgat gtggatcgcc accatggtgc tgattgccgt tgtggtttat
     5041 ctcctgcgtt atgtctggcg cgtattgctg tttggcgcgt cttaccaact ggctgttgaa
     5101 ctgcgtgaag attattaccg tcagctaagc cggcagcacc cggagtttta cctgcgtcat
     5161 cgcacgggtg atctcatggc tcgtgcgacc aatgacgtcg attgcgtcgt gtttgccgcc
     5221 ggagaagggg tgctgacgct ggtggattca ctggtaatgg gctgcgctgt gttgattatg
     5281 atgtctacgc aaattagctg gcagttgacc ttatttgccc tgttgccgat gccagtgatg
     5341 gcgatcatga ttaagcgcaa cggcgatgct ttgcatgaac gctttaagct ggcacaggcg
     5401 gcgttttcca gtcttaatga ccgcacccag gaaagcctca ccagtatccg catgatcaaa
     5461 gcctttggtc tggaagatcg ccagtcggcg ttatttgccg cggatgccga agataccggc
     5521 aaaaaaaaca tgcgggtggc gcgtattgat gctcgtttcg acccgaccat ctatatcgcg
     5581 attggtatgg cgaacttgct ggcgattggc ggcggtagtt ggatggtggt gcagggcagt
     5641 ttaacgctgg gccagctcac cagttttatg atgtatttag gtctgatgat ttggccaatg
     5701 ctggcgctgg catggatgtt taacattgtg gaacgtggta gtgctgcgta cagccgtatt
     5761 cgtgcgatgc tggcggaagc gccggtggtg aacgatggta gcgaacccgt gccggaaggg
     5821 cgtggcgaac ttgatgtaaa tattcaccag ttcacgtatc cgcagactga ccatcctgcg
     5881 ctggaaaacg tcaatttcgc cctgaaaccc ggtcagatgc tgggtatctg cgggccgact
     5941 ggttccggca aaagtaccct gttgtcgctc attcagcgtc atttcgacgt tagcgagggg
     6001 gatattcgct ttcatgatat tcctctgacg aagttacaac tcgatagctg gcgtagccgc
     6061 ctggcggtgg tcagccagac gccattcctt ttttctgaca ccgtggcaaa taacatcgcg
     6121 ctgggttgcc cgaatgccac ccagcaagag attgagcatg tcgcgcggtt agccagcgta
     6181 catgacgata ttttgcgttt accgcaaggt tacgatacag aggtgggcga gcgcggcgtg
     6241 atgctttccg gcgggcaaaa acagcgtatc tctattgctc gtgcgttatt agtcaacgcg
     6301 gaaatcctca tccttgatga tgcgctttcg gcggtggacg gacgcacaga gcaccagatc
     6361 ctgcataacc tgcgtcagtg gggacaggga agaacggtaa tcatcagtgc ccatcgcctt
     6421 tcggcactga cggaagccag tgaaattatt gtgatgcagc acggacatat cgcccagcgt
     6481 ggcaatcatg atgtgctggc gcaacaaagc ggctggtatc gcgatatgta tcgctatcaa
     6541 caactggagg cggcgctcga cgacgctccg gaaaatcacg aggaggccgt cgatgcgtag
     6601 ttttagccaa ctgtggccga ctctcaagcg cctgttagcg tacggttcac cgtggcgtaa
     6661 accgctgggg attgcggtcc tgatgatgtg ggttgcggcg gcggcagaag tcagtgggcc
     6721 gctgcttatc agctatttta tcgacaatat ggtagcgaaa aataacctgc cgttgaaagt
     6781 ggttgcaggg ctggctgcgg cgtatgttgg gctacaactg tttgccgccg ggctacatta
     6841 cgcgcagtcg ctgctgttta atcgggcggc agtaggcgta gtgcaacagt tgcgtaccga
     6901 cgtgatggat gctgcgttac gccagccatt aagcgagttt gatacccaac ccgtcgggca
     6961 ggtgatttcc cgcgtcacta atgacactga agtgatccgc gatctctacg ttaccgtagt
     7021 ggcaactgtc ctgcgcagtg ccgcactggt gggcgcgatg ctggtggcga tgttcagcct
     7081 cgactggcga atggcgctgg tggcgataat gattttcccg gtagtgctgg tggtaatggt
     7141 gatatatcaa cgttacagca cgccgattgt ccgtcgtgtg cgcgcctatc tggcggatat
     7201 caacgacggc tttaacgaaa tcatcaatgg catgagcgtc atccagcagt tccgtcagca
     7261 ggcgcgattt ggcgaacgta tgggtgaggc cagtcgttca cactatatgg cgaggatgca
     7321 aaccctgcgc ctcgacggtt ttctgctgcg tccgctgctg agtctgtttt catcgctcat
     7381 tctttgtgga ttgttgatgc tgtttggctt ctccgccagc ggcaccattg aagtgggcgt
     7441 gctgtatgcc tttatcagct atcttgggcg acttaacgaa cccttaatcg aactgaccac
     7501 gcaacaggcg atgctgcaac aggctgttgt tgctggtgag cgcgtgtttg aactgatgga
     7561 cggaccgcgc cagcaatatg gcaatgatga ttacccgtta cagagtggca ccatcgaagt
     7621 cgataacgtg tcatttgctt atcgcgatga caatctggtg ctaaagaaca ttaatctctc
     7681 tgtgccttcg agtaactttg tggcgctggt cgggcatacc ggcagtggta aaagtaccct
     7741 cgccagttta ttgatgggct attacccgct aacggaaggt gagattcgcc ttgatggtcg
     7801 tccattaagt tcgctaagtc acagcgcgct gcgccagggc gtggcaatgg tgcagcaaga
     7861 tccggtggtg ctggcggata ccttcctcgc caacgtgacg ctggggcggg atatctccga
     7921 agaacgcgtc tggcaggcgc tggaaaccgt gcaactggcg gagctggcgc gtagcatgag
     7981 cgacggtatt tacacgccgc tgggcgagca ggggaataat ctctcagttg ggcaaaagca
     8041 actgctggca ctggcgcgcg tgctggtcga gacgccgcaa atcctgatcc ttgatgaggc
     8101 aaccgccagc attgactccg ggactgaaca ggcgattcaa catgctctgg cggcggtgcg
     8161 tgaacatacc acgctggtgg tgattgctca ccgcttatcg accattgttg atgccgacac
     8221 cattctggtg cttcatcgtg ggcaagccgt ggagcagggc actcaccagc aactgctggc
     8281 tgcccaggga cgctactggc agatgtatca actgcaactt gcgggcgaag agctggcagc
     8341 cagcgtgcgt gaagaggaat cattgagcgc ctgaatagcg caatatttca tcgttggtgc
     8401 aaaaatgtaa cgcactgtgc actgtcatag tgcgttttca ttttcaaact tcttaacttc
     8461 ctgctctctt tctcgttttt catttctggc acaccgcttg caataccttc ttcgtgtagc
     8521 agaaccatta ccgaattctg accggagggg atctatgaag ctggtgaccg tgataatcaa
     8581 accattcaag ctggaagacg ttcgtgaagc gttatcttcc attggtattc agggcctgac
     8641 cgtcaccgaa gtgaaaggtt tcgggcgtca gaaagggcat gccgagctgt accggggggc
     8701 ggaatacagc gtcaacttcc tgccaaaagt aaaaattgat gtggcgattg ctgatgacca
     8761 actcgatgaa gtgatcgata tcgtcagtaa ggcggcttac accggaaaaa ttggcgacgg
     8821 caaaatcttc gtcgctgaat tgcaacgcgt cattcgtatt cgtaccggcg aagccgacga
     8881 agcggcgctg taatctctgg cacacagcaa caggaacgaa aaatgaagat agcgacgata
     8941 aaaactgggc ttgcttcact ggcgatgctg ccgggactgg taatggctgc acctgcggtg
     9001 gccgataaag ccgacaatgc gtttatgatg atttgtactg cgctggtgct gtttatgact
     9061 attccgggga ttgccctgtt ttacggtggg ttgattcgcg gcaaaaacgt gctgtcgatg
     9121 ctgacgcagg tgacggtgac gtttgcactg gtctgtattc tctgggtggt ttacggttac
     9181 tcgctggcgt ttggtgaggg caacaacttc ttcggcaaca ttaactggtt gatgctaaaa
     9241 aacatcgaac tgacggcggt gatgggcagc atttatcagt atatccacgt ggcgtttcag
     9301 ggctcgtttg cctgcattac cgtcggcttg atcgttgggg cgctggcgga acgaatccgc
     9361 ttctcagctg tgttgatttt cgtggtggta tggctgacgc tctcttacat tccgattgcg
     9421 catatggtgt ggggcggtgg tttgctggct tctcacggtg cgctggattt cgcgggtggc
     9481 acggtggtgc atattaacgc cgcaatcgcc ggtctggtgg gcgcgtatct gataggcaaa
     9541 cgcgtgggct tcggcaaaga ggcgtttaaa ccgcacaacc tgccaatggt cttcaccggt
     9601 actgccattc tctatattgg ttggtttggc tttaacgccg ggtcagcggg cacggcgaat
     9661 gaaatcgcgg cactggcatt tgtgaatact gtggtcgcaa cggcggcggc aattcttggc
     9721 tggatcttcg gtgaatgggc gctgcgtggt aagccttctc tgctgggagc gtgttctggc
     9781 gcgattgccg gtctggtcgg cgtgacgcca gcgtgtggct acattggggt tggcggcgcg
     9841 ttgattatcg gcgtggtagc tggtctggcg ggcttgtggg gcgttactat gctcaaacgc
     9901 ttgctgcggg tggatgatcc ctgcgatgtc ttcggtgtgc acggcgtttg tggcattgtc
     9961 ggctgtatca tgactgggat ttttgccgcc agttcgctgg gtggcgtggg cttcgctgaa
    10021 ggtgtgacga tgggccatca gttgctggta cagctggaaa gcatcgccat tacgatcgtc
    10081 tggtccggtg tggtggcatt tattggctac aaactggcgg atctgacggt tggtctgcgt
    10141 gtgccggaag aacaggagcg agaagggctg gacgtcaaca gccacggcga gaatgcctat
    10201 aacgcgtaac aagcactgca aaaaacagcc ggacggtttt cacctccggc tattttttta
    10261 attgtgatta cgcatcaccc cttcctgaac ggtcgaggca accagcacgc cgtcctgagt
    10321 ataaaactca ccgcgcacaa agccgcgtgc gctggacgcc gaggtgctct ccacgctata
    10381 cagcagccat tcattcaaat taaacgggcg atgaaaccac atggaatggt caatggtggc
    10441 aatctgaatc cccggttcga gaaaaccgat gccgtgcggc tgtagagcta ccggcaggaa
    10501 gttaagatca gaagcgtaac cgagcagata ctgatgaacg cgcaggtcat ccggcacgct
    10561 accatttgcg cggatccaca cctgacgatg tggttctgcg acgtgacctt tcagtgggtt
    10621 atgaaactcc accggacgga cttccagcgg acgatcgcag atgaatttat ctttcagcac
    10681 tggcggcagc aggtgcgcca gcgattgggc gatttgtgtt tccgaaggca ggccatcagg
    10741 cgctggcgcg gacggcattg ttttttgatg ttcgaaaccc gcttcaggtg cctggaaaga
    10801 ggccgtcata taaaaaatcg gtttgccgtt ttgaatagca gcaacccggc gggcgctgaa
    10861 gctgttaccg tcacgcagcg tttcgacatc ataaataatc ggcttcttac tatcgccagg
    10921 gcgaagaaag tagctgtgaa acgaatgtac cagccgctct tcagggacgg tctcttttgc
    10981 agcatacaag gcctgaccca cgacctggcc gccaaacacc tggcgtaaac ctaaatcttc
    11041 actctggccg cgaaagagtc cttcctcaat tttttccaga tttaacaatg tcagtaaatt
    11101 ttttagcgcc tgactcatat aactctccag taactaagct gccgcagcaa gccaaagtga
    11161 gttgagtata acgcaaattt gccactggtc cgatgggtgc aatagtctga attacgggct
    11221 aattacaggc agaaatgcgt gatgtgtgcc acacttgttg acgttactat tttgttaacc
    11281 actcttccgg cgaggaaagt tagcccgctg gtgcattgat aataaggaga agtgaatgaa
    11341 actcgtgcac atggccagtt gtttagcggt tgcgattgcg ttggcggctt gcgcagataa
    11401 aagcgcggat attcagacgc cagcaccggc ggcaaatacg tctatttcag caacacaaca
    11461 acctgctatc cagcaaccga atgtctccgg taccgtttgg atccgtcaga aagtcgcact
    11521 gccgcctgat gctgtgctga ccgtgacact ttctgacgcg tcgttagccg atgcgccgtc
    11581 aaaagtgctg gcgcagaaag cggtgcgtac cgaaggtaaa cagtcaccat tcagctttgt
    11641 tctgcctttt aacccggcag atgttcagcc gaacgcgcgt attctgttga gtgcggcgat
    11701 taccgtgaat gacaaactgg tatttattac cgataccgtt cagccggtga tcaacaaggg
    11761 cggaactaaa gccgacctga cattggtgcc gatgcagcaa accgccgtgc cggttcaggc
    11821 cagcggtggt gcaacgacta ccgtaccttc gacttcacca actcaggtga atccgtcttc
    11881 ggcagttcca gctcctacgc aatattaagc gacgttaacc ctctccgccc ggagagggtt
    11941 agtagttcca gcgataacgc tgcaagtcga tttgcccgct tcccgatacc atcacacctt
    12001 ctgccagtaa tgcctgtcgc tgacgctgta aatccggtcc ggttagcgaa attgtgccgt
    12061 ggcgattaac cacccggtgc cagggtaagg tgctgccttc agggagacgc tttaacacac
    12121 cgcccacctg gcgcgcggcg cggggcgatc ccgccagttt cgccacatca ccgtaagtgg
    12181 tgacatagcc ttcgggaata gcggcgacga tttgccagac gcgttgggga aatgaatctt
    12241 ctttttccat cttttcttcc tgaggtaatt tttcagcata atctggaaaa acgcccgagt
    12301 gaagtcgcat tgcgcaagaa accagcatct ggcacgcgat gggttgcaat tagccggggc
    12361 agcagtgata atgcgcctgc gcgttggttc tcaacgctct caatgggggc tctgttggtt
    12421 ctcccgcaac gctactctgt ttaccaggtc aggtccggaa ggaagcagcc aaggcagatg
    12481 acgcgtgtgc cgggatgtag ctggcagggc ccccacccat ttctgcctcc caccgtttcg
    12541 tcaaaaaatc ccaacatagc taaactttaa ccacaactga cgtcgcaaga attgtctggc
    12601 tgcgcagtac gcttcggagg tatgtctgat gaagtatgtt gatggttttg tggttgccgt
    12661 tcctgccgat aaaaaggatg cctatcggga aatggccgct aaggctgcgc cattgtttaa
    12721 agagtttggc gcgcttcgta ttgtcgaatg ctgggccagc gatgtaccgg atggcaaagt
    12781 gaccgatttt cgtatggcgg tgaaagcgga agagaatgaa gaggttgtct ttagctggat
    12841 tgaataccct tcaaaagagg tccgcgacgc tgctaatcaa aagatgatgt cggacccacg
    12901 gatgaaagag ttcggcgagt ccatgccgtt tgacggcaag cgaatgatct atggcggatt
    12961 cgagtcaatc atcgacgaat aggtagcgtg acgggctgtg cggcgtgccg tcagcccaaa
    13021 aattcacaaa tgttgctcgg cccagcgtaa gaaatcttct ttcggtaatg ccttgctgta
    13081 gagccagccc tgaccgtagt gcacgccatg ctggcgtagc cactcttctt gtttactggt
    13141 ttcgattccc tccgctacca ttttcagttt cagtgttttt gccatttcga tgatatgcgg
    13201 cgtgacattt ttatattcca gcgcatcaac gaaagattta tcgatcttca gaatgtcgac
    13261 atccagatcc tgtaaataac ttaaacttga atatcccgta ccaaaatcat caagataaat
    13321 ttcatggccc gcctcccggt agcgagaaat tatcggggcg ctggttttcg gatcggcaaa
    13381 ctcgcgttca gtgagttcaa gcgcgatctg tctgggatta acctgattga ttgatcattt
    13441 cacgcagcaa ttgcgggatt ttttccgagg tgagcacggt ggattcaaga ttgatcgaaa
    13501 tatgctgctg tggatgctga cgcagccagt cgcccatatc ttcaaaggcg cttcttataa
    13561 tcagtagcgt caatggctca gaaaggcccg tttgctgtgc cagcggaata aaactatctg
    13621 gtgacaacca actaccgtct gtctgcggcc agcgcgccag tgcctcagca ccgacaattt
    13681 tgccattggc taaggagaca atcggctgat agtgcacgca aatatcacga ttttcgatag
    13741 catcctgcag ccgatgatgc ggtgactgga tacggcgcaa aatacgcagc acaaacatcg
    13801 ctgccagcag gccaatcacc aacccgagcg gtaaccaaat aaagacttgc cgatgccagc
    13861 ctttctgcaa cattttcgtt gaagcccagg tgatgatcga aatattcatc tccgggaagg
    13921 gcaggatatc gtaaatgatt ccattatttt cgatatgctc accgggtgtt ttttgtagcc
    13981 tggtaataat tccctgagca atttcatcgc tgctggttat gacaacgtta tgggcattgc
    14041 caataatggc ggcatcaatt tgccatgagc tatagggaat gacatcaata aaggaagcgg
    14101 ggtcgatcat gacgacataa tgtgccgttc ccatggcgac catgtaacgg ataatgccta
    14161 aatcgttatg cgatgttaac cagacacgat aaccatcttt cgaaatttta cctggctcgg
    14221 ggaaggtatc gggcggactt tcatgctcca gagacgaaca ctggggaacg ttgttatcga
    14281 tataaaccac ttcctgaata tagcgataac tgtaagatac ccgacgcatt tccatgagat
    14341 gggcttcgct acaggcagcg ccttgccatc tttccagctc ttgcagcgca tctttccctt
    14401 gtgtcgccac cttattggcc cgaatagcga cgcgggagga ataggtatcc agctcttcaa
    14461 taaacgatgt ttctacctgc tgatgggcca gccagatgct taagccgaca ggcagcaata
    14521 ctgaaagaat cagtactccc gaaataaggc cgaccagatg tcgtgttctc accagcatat
    14581 ccttattaac acagtgtagg gaaagtatat ctgattgtgg aaaaaggctg gcaggagata
    14641 aggaggtaga gctggtattt catgaagtga tgggtggaaa cgatcattgt catttgacca
    14701 agcgcagagc tttacgcctg gtatgtaacc ctaacttaag atgttgattc ggcgcgagcg
    14761 agggtaaaaa tatcgtaaaa agacagttca cctttacgga cgatcatttt ttgcagttgt
    14821 tccttatgaa catccgcgac acgcggagag ttcaagatgg cttcaaccag cgcggcaggt
    14881 acaaagcgcg tgttgtacca ttcgccgtta tagcaaacgc ggaaatccag cacgtcgata
    14941 tcttcatagc ggtagcgtgg gtagacatcg aaaaagaaga aaccattaag caggataaat
    15001 aatacaacca gtagccagac agagcctgac agcgtttcgg actgcaacat caccgccagg
    15061 gtggcaaaaa aagcaacgta cataccgata aacagccccg gatgtttacg gataaaactg
    15121 atactaaagc ggggtttgtt gtcgcgtttt tcgcgggtat tcagagactc aatcgtttcg
    15181 gtcagcaggc gttgtatttc ggtcatcgtt tgccttcgtg gtatctgcaa ctttccaggg
    15241 aacaccctat tttaacgtgg gaatagtaga ggggaagtaa cagatttacc acaattttgc
    15301 ataacagttg cgaaaaaccg attacaattt tttaattatt ctggctggat taccgcccac
    15361 gacaacgttg tccgggacat cttttgtgac aactgcacct gaggctacca cgacgttatc
    15421 accaatggtc acaccagggt taatgaccgc gcgtccgcca atccagacgt tattaccgat
    15481 ggtgacgggt ttccccagtt cagcaccgct attacgtgct acagggtcga tgggatgtgt
    15541 tgccgtgtag atatgaacgc ctggtgccaa catacagtta tcaccgatgc gaatagggca
    15601 gacatcaagc atcacgcaat cgaagttggc gaaaaaatta ttaccgagaa aaatgttata
    15661 gccatagtca cagcgaaacg ttggctcaat ataagcctct gtcacctgac cgaatagatc
    15721 agcgagaatt tgctggcgta atgtgtgctc ttccgccagg gaatgattgt atcggtgaat
    15781 aagctgacga gcgcgcaggc gatcgcgaga taacgtctca tctgccgagc gatacaactc
    15841 accagcaatc atcttttctt tttctgtgct cattactgac ctccggttgc tgaaaatgcg
    15901 gcaacacagt aagacgaaaa gtcagtgaag agaacgttcc cattacatct gtgtgataaa
    15961 gatcacatag ggtatgtctt catggtgaaa agtataaaat tcttaataaa cagccggtta
    16021 tagctccgaa agcgaattat ttattagcga ataaatttcc atactgagga agggatcttg
    16081 tcgtatagtt tattcatggt caattcggcg aggcggtgat ctgcggctga gtaaaatacc
    16141 gccagttcat tatctgataa ttcgtattta tttttctcga taacacgctc cagcgtgtca
    16201 attgtctggc aacgacgtaa acgcattaaa taatcggttt tcgttaaagg tttttcggac
    16261 atacttctac ccatggttgt aataattcta acaagataaa ctcgcaggat tctctttcgt
    16321 cgcattgaca aaacaacgga atagtcgatt acctgacttc cgccatttct gaagatcctg
    16381 catattaata ccataactac tgaacaacat aaaggtgtca tccagatatt cgtcgatctg
    16441 ctcaatgagc ttattgtctt cattatactt aattttgtaa ttaagtgcga aggtcgcaat
    16501 atgctcaatc agttcattca actggaggtt gatcgccgag gttgggtcgt taacccagcc
    16561 atgattgctt tcttcaaggt ttgcaaggca gtcatgatac agggtttcac agagaaactt
    16621 aagctgtgcg atatcatgtc ttttgggtga gtattcatcc ataacgcgtc cccttcttag
    16681 cggttgaact aacggacacc tttcgggatg gaaaaaactt actgacctgg acttgccctc
    16741 tcgtttgtta gcttaactat aagccactct ttgcaggttt tcatcgcatt attacgaaaa
    16801 attacaatta ggaaggaatc gggacaggac tcactttttc atactagcac taacccgcag
    16861 caggtaaaag cagtctgctc gaactgttca taacatttca ggattatcgc cagatgaaat
    16921 tggtgacact aataccagga ttgctctgaa tatgacgtaa taaccgagga atgaataaag
    16981 aattaccgca aataattaag aatagcctct aaatgattat ggattattaa atctattagc
    17041 gctacttaat ataaattaac taacaatcag tattttttat gaattttatc cgtggttaat
    17101 actggttttc gtatgagatc ctgagttggt ggttcaatta ctccttaatg ttcgtaggtt
    17161 atgcataaaa aaggccgctt gcgcggcctt agtgattaca cgttgtatca atgatgatcg
    17221 acagtatggc tgtgctcgat atcttcattc ttgcggctaa agcggcggcg aaccaccaca
    17281 aagaataccg gaacgaagaa gattgccagt accgttgcgg tcaccatccc gcccattaca
    17341 ccggtaccta ctgcgttctg cgcgccggaa ccagcaccag tactgataac cagcggcata
    17401 acgccgagga taaacgccag cgaggtcatc aggatcggac gtaaacgcat ccgcactgca
    17461 tcaagcgtcg cttcaatcag acctttacct tctttatcca tcaagtcttt ggcgaattcg
    17521 acgataagta tcgcgttctt cgccgacaac ccaatggttg tgagcaggcc tacctggaag
    17581 taaacgtcat tggtcaggcc acggaaggtg gcagccagca acgcaccgat aacccccagc
    17641 ggaacgacca gcataacgga gaacggaatc gaccagctct cgtacagcgc cgccagacac
    17701 aggaatacga caatcaacga aatcgcgtac agtgaaggtg cctggttgcc ggagagacgt
    17761 tcctgatagg acatccccgt ccagtcatag ccaacaccgg taggcagttt gctcgccagt
    17821 tgttccatca gctccattgc ttcaccggta cttttacccg gtgccgcctg gcctaagatt
    17881 tccatggatg gcaggccgtt gtaacgttcc agacgcggcg aaccgtactc ccaacgagaa
    17941 gaggagaacg ccgagaatgg caccatctga ccatcagcag cacgaacata ccagtcgccg
    18001 atatcatccg gcagcatacg gtatttcgct tctgacatga cgtaaacttt cttcacacga
    18061 ccgcggtcga taaagtcgtt cacatagcta ccgccccatg cagcgcccag agaggtgtta
    18121 atgtcgttga tagaaacacc cagcgcctgc gctttttcct ggtcgatatc aatcttaaac
    18181 tgcggggtat cttccagacc gtttggacgt acgctggtca gcatatcagg gtgcttcgct
    18241 gcttctgcaa gcagctggtt acgcgcctga gtcagttttt cgtgaccaag gccagcctgg
    18301 tcaatcagct caaagtcaaa gccggttgca gtacccagtt ccacgattgc gggcaggtta
    18361 aaggcgaaaa ccatcgcatc tttgatttgc gagaaagcgc gtgttgcacg catggtaatc
    18421 gcttcaactt tgttttcttc gcccggacga tcggcccagt ccttcaagga aacgaacgca
    18481 ataccggtat tctgaccacg tcccgcaaag ccgaagccgt taacggcgaa caccgactca
    18541 acgttgttct tttctttggt cagatagtaa tgcgttacct cattgagcac tttctgcgta
    18601 cgttcctgcg ttgcacctgc tggcagctga accatggtca taaatacgcc ctggtcctca
    18661 tctggcaaga aggagcttgg cagacgcacg aacagatagg ccatgccgac cacgatgatc
    18721 agatacagca ccaggtaacg ccccgtactg cgcagaatac cgcctacgct gtcggtgtag
    18781 tggtgcgtgc tcttctcgaa catgcggtta aaccagccga agaagccttt tttaccttcc
    18841 ccgtgatcgc ctttggcaat cggtttcagc atggtggcac aaagagccgg agtcaggatc
    18901 aacgctacca gtaccgacag cgccattgct gaaacaatgg taatagagaa ctgacgatag
    18961 atagcaccag tagaaccgcc aaagaaggcc atcggtacga ataccgccga cagtaccatc
    19021 gcgataccga ccagagcgcc ctgaatctgc cccatcgact tacgggtagc ttcttttggc
    19081 ggcaaacctt cttccgccat aacacgctcc acgttttcta ccacaacgat agcgtcatcc
    19141 accaacaagc cgatggcgag caccatcccg aacattgtta gcgtgtttat cgagaagcca
    19201 aaggcggcaa ggacggcaaa ggtcccgagc aataccaccg gtacggcaat ggtcggaatc
    19261 aacgtcgcgc ggaagttctg caggaacaga tacataacca ggaacacgag gatgatcgct
    19321 tcgaccagcg ttttaaccac ttcgtgaata gagattttca cgaacggcgt ggtgtcgtac
    19381 gggtaaacaa ttttcagacc cgacgggaag aacggttcca tcttcgccag ttcagcacgg
    19441 attgccgcag cggtatccag cgcgtttgca ccggtcgcca gcttgatccc cagaccggaa
    19501 gccggttggc cgttaaactc tgcgatgatg tcgtagttct caccacccag ctcaatcttc
    19561 gccacatcac gcagcagtac gcgggaacca tcctgattca ctttcagcag gattttgccg
    19621 aactcttcag tagaggtcag acgcgtctga gcaataatag aggcgttaag ctgttggcct
    19681 ttcaccggcg gcgtaccacc gagctgaccc gccgcaacct gggcgttctg cgctttgatg
    19741 gcggtaatga catcaaccgg cgttagctgg aatttgttca gctcattcgg gttcatccag
    19801 atacgcatcg cgtactgtga accgaacaac tgaacatcac ccacgcccga cgtacggctg
    19861 atggcatctt tcatattcgc cgctacgtag tcagagatat cctcctgcgt catggtgcca
    19921 tcggtgttga taacgccgac aaccatcagg aagctgctgg atgatttctc aacgctcacc
    19981 ccttgctgct gaacttcttg cggcagcaac ggcatcgcca gctgcagttt gttctgcacc
    20041 tgaacctgcg cgatatccgc atcagtacca gactcaaagg tcagggtgat ctgcacggta
    20101 cccgtggagt cactgttaga ggacatgtac atcaggttat cgataccgtt catattctgt
    20161 tcgataacct gtgtcaccgt gtcctgcact gttttcgcat cagcgccggg gtaggaggcg
    20221 gagatcgtta ctgccggcgg tgcaatcgta ggatattgcg ccaccggcag tttgaggatc
    20281 gccagccccc ctgccaacat gatgataatg gcgatcaccc acgcaaaaat cgggcgatcg
    20341 ataaagaaat taggcatgtc ttaacggctc ctgtttaagt taagacttgg actgttcagg
    20401 ctgagcaccg cttgcggctt gctggttatt atcagcggta acttcttgtg cttttacctg
    20461 gacaccagga cgcactttct gcagcccact tattactacg cgatcgcctg ctttcagacc
    20521 ttctgtcacc agccacttat cgccaatagc ctggcttgca acgatcggac gggtttccac
    20581 tttgtcatcc gcgccaacta ccagtacggt ggcatcacca cgcggcgtac gggttacgcc
    20641 ctgttgcggg actaaaatag cgtttggatt aagcccttct tccagacgtg cgcgcacgaa
    20701 catacccggc agcagagtgt gatccgggtt cgggaagata gcgcgtaggg tgatagaccc
    20761 agtggtctga tcaacggtaa cgtcagagaa ttccagcgta ccgtcctgcg ggaacttaat
    20821 gccgtcactg gtgatcagcg acactttggc tttgccgttc tcttgtttca gcgtgccatt
    20881 cgccagttcc tgtttcaggc gcaggaagtc gttgctggac tgggtcacat caaggtagat
    20941 cggatcaagt tgctgcacgg ttgccagcgc agtcgcctga ccgttctgta ccaatgcgcc
    21001 ttccgtcacg ttcgacttac caatgcgacc gctaatcgga gaggtgactt tggtgtaagc
    21061 cagattgatc cgcgcagttt caacggcagc tttcgccgca gttaccgcag cattcgcctg
    21121 ttgcgcatca gccagagcct gatcgtactc ttgcttactg atgtactgag taccgagcag
    21181 tttctgataa cgattcaccg tcaattgcgc gatattggct gcagcctggg ctttcgccag
    21241 atcacctttc gcactgtcgt atgtcgcctg ataggtcgca ggatcaatct gatagagaga
    21301 gacaccagct tcgatgtcgc taccttcttt gaaattacgc ttcaggataa tcccgctaac
    21361 ttgaggacga acttctgcga tccggtaggc actggtgcga cccggaagct cggttgtgat
    21421 ctgcagaggt tcagttttga ctgttactac gccaacggcg ggcatctgct ggccaccttg
    21481 ttgggcctgt ttgtcgtcac atcctgttag ggctaagctg cctgagagca tcagaacgac
    21541 cgccagaggc gtaaaccctc tgtttttgtt catatgtaaa cctcgagtgt ccgatttcaa
    21601 attggtcaat ggtcaaaagt taataaaccc attgctgcgt ttatattatc gtcgtgctat
    21661 ggtacataca ttcacaaatg tatgtaaatc taacgcctgt aaattcacga acatatggca
    21721 cgaaaaacca aacaagaagc gcaagaaacg cgccaacaca tcctcgatgt ggctctacgt
    21781 cttttctcac agcagggggt atcatccacc tcgctgggcg agattgcaaa agcagctggc
    21841 gttacgcgcg gtgcaatcta ctggcatttt aaagacaagt cggatttgtt cagtgagatc
    21901 tgggaactgt cagaatccaa tattggtgaa ctagagcttg agtatcaggc aaaattccct
    21961 ggcgatccac tctcagtatt aagagagata ttaattcatg ttcttgaatc cacggtgaca
    22021 gaagaacgac gtcgattatt gatggagatt atattccaca aatgcgaatt tgtcggagaa
    22081 atggctgttg tgcaacaggc acaacgtaat ctctgtctgg aaagttatga ccgtatagaa
    22141 caaacgttaa aacattgtat tgaagcgaaa atgttgcctg cggatttaat gacgcgtcgg
    22201 gcagcaatta ttatgcgcgg ctatatttcc ggcctgatgg aaaactggct ctttgccccg
    22261 caatcttttg atcttaaaaa agaagcccgc gattacgtcg ccatcttact ggagatgtat
    22321 ctcctgtgcc ccacgcttcg taatcctgcc actaacgaat aaccctgaat ctgactccag
    22381 gatttttcct ggacattttc gtcgttgcta ttctggttca ctgcgtcgtg atattcttgc
    22441 ggtttgactt tttcaggtcg ttcttcaggt tcagaaacct tcattcatca tgactatgtt
    22501 ccagtattac aaacgatcac ggcattttgt tttttcagca tttattgctt ttgtttttgt
    22561 cttgttatgc cagaacacgg cgtttgcgcg ggcgtcatcg aatggtgatc tgccgacaaa
    22621 agcggacctg caggcgcaac ttgactcact aaataaacaa aaagatcttt ctgctcagga
    22681 caaactggtg cagcaggatc tgacagatac attagccacc ctcgataaaa tcgatcgcat
    22741 aaaagaagag acagttcagc tacggcaaaa agtcgctgaa gcgccggaaa aaatgcgcca
    22801 ggcgaccgcg gcgttaacag cacttagcga tgtcgataac gacgaagaaa cgcgcaaaat
    22861 tctgagtacg ctttcgttgc gccagctgga aactcgcgtt gcccaggcgc tggacgattt
    22921 gcaaaacgca caaaacgatc tggcgtctta taacagccag ctggtttcgt tacagacgca
    22981 gcccgaacgc gtgcaaaatg cgatgtataa cgcttcgcag cagctgcaac aaattcgcag
    23041 tcgtctggat gggactgatg tcggcgagac tgccttacgt cccagccaga aagtgttaat
    23101 gcaggctcag caggcgttgc tgaatgcgga gattgaccag cagcgtaaaa gcctggaagg
    23161 gaacaccgtc ttgcaggata ccttgcaaaa gcaacgtgat tacgtgacgg cgaacagcgc
    23221 ccgtctggag caccagttgc agctgttgca agaagcggta aacagcaagc gcctgacttt
    23281 aactgaaaaa acggcgcagg aagccgtctc cccggatgaa gccgcgcgta ttcaggctaa
    23341 tccgctggtg aaacaggaac tggaaattaa ccagcagtta agtcagcgtc tgattaccgc
    23401 gactgaaaac ggtaatcagt tgatgcagca aaacattaaa gtcaaaaact ggctggagcg
    23461 ggcgctgcaa tcggaacgca atattaaaga gcagattgcc gtcctgaagg gcagcctgct
    23521 gttgtctcgt atcctttacc agcaacaaca aacgctgccc tcggcggatg aactggaaaa
    23581 catgaccaac cgcatcgcgg atttgcgtct cgaacagttt gaagttaacc agcagcgtga
    23641 tgcactcttc cagagcgatg cgttcgtcaa caaactggaa gaaggtcaca ccaacgaagt
    23701 caacagcgaa gttcacgatg cgttattgca agtggttgat atgcgtcgcg aattgctgga
    23761 tcaactcaac aaacagttgg gtaaccagct gatgatggcc attaacctgc aaatcaacca
    23821 gcagcagtta atgagcgtat cgaaaaacct gaaatccatc ctgactcagc aaatcttttg
    23881 ggtgaacagt aaccgtccaa tggactggga ctggatcaaa gcgttcccgc aaagcctgaa
    23941 agatgaattt aagtcgatga aaatcacggt gaactgggaa aaagcctggc ctgccgtttt
    24001 tatcgctttc ctcgccggtt tgccgctgct gttgattgcc gggctgatcc actggcgtct
    24061 gggctggctg aaagcgtatc aacagaaact ggcttccgct gtgggttccc tgcgtaacga
    24121 tagccagctc aacacaccaa aagcgatcct tatcgacctg atccgcgcgc tgccggtgtg
    24181 cctgattatt ctcgcggttg gcctgattct gttgaccatg cagctcaaca tcagcgaact
    24241 gctatggtcg ttcagtaaaa aactggcgat tttctggctg gtgtttggcc tgtgctggaa
    24301 ggtactggag aaaaatggcg ttgccgtgcg tcacttcggc atgccggagc agcagaccag
    24361 ccactggcgt cggcaaattg tccgcatcag tctcgcgttg ctgcctatcc atttctggtc
    24421 tgtggtggca gaactttccc cgctgcatct gatggatgat gtgctggggc aggcgatgat
    24481 tttcttcaac ctgctgctga ttgccttcct ggtatggcca atgtgccgcg aaagctggcg
    24541 tgataaagag tcgcacacca tgcgactggt caccattacc gtgctgtcga ttatcccgat
    24601 tgcattgatg gtgctgactg ccacgggtta tttctacacc acgctgcgcc tctccgggcg
    24661 ttggattgaa accgtttatc tggtgatcat ctggaacttg ctctaccaga cggtattgcg
    24721 cggcttaagc gtagcggcgc ggcgtatcgc ctggcgtcgt gcgctggcgc gtcggcagaa
    24781 tctggtgaaa gagggcgcag aaggtgccga accgccggaa gaacccacca ttgcactgga
    24841 acaggttaac cagcagacgc tgcgtattac catgttgctg atgtttgcgc tgttcggtgt
    24901 catgttctgg gcaatttggt ccgatttgat caccgtgttc agctatctcg acagcatcac
    24961 gctctggcat tacaacggca ctgaagctgg cgctgcggtg gtgaaaaacg tcaccatggg
    25021 cagtctgttg tttgcgatta tcgcctcaat ggtggcctgg gcgttgattc gcaacctgcc
    25081 tggtttactg gaagtgctgg tgctctcgcg actgaatatg cgtcagggcg cgtcgtatgc
    25141 cattactacc atccttaact acatcattat tgccgttggt gcgatgacgg tgttcggatc
    25201 gctgggcgtc tcttgggata aactccagtg gctggccgca gcattatccg taggtcttgg
    25261 ttttggttta caagaaattt tcggtaactt cgtctccggt ttgatcattc tgttcgaacg
    25321 tccggtgcgt attggcgata cggtaaccat tggtagcttc tcggggacgg taagtaagat
    25381 ccgtattcgt gcgacaacga ttaccgattt cgatcgcaaa gaagtgatca tcccgaacaa
    25441 agcgtttgtt accgagcgtc tgatcaactg gtcgttgact gacactacta cgcgtctggt
    25501 gatccgtctc ggcgtggcct atggctccga tctggaaaaa gtgcgtaaag tgttactgaa
    25561 ggcggcgact gagcacccaa gggtgatgca cgaaccaatg ccggaagtct tctttacggc
    25621 gtttggtgcc agcacgttgg atcatgagct gcgtctgtat gtgcgtgaac tgcgtgaccg
    25681 tagtcgtact gtcgatgagc tgaaccgtac tatcgatcag ctgtgccgtg aaaacgacat
    25741 caacattgcc tttaaccagc ttgaagtgca tctgcacaac gagaagggcg atgaggtgac
    25801 ggaagtcaaa cgcgactaca aaggcgatga cccgacgcca gcggtagggt aataaccaaa
    25861 gggggcgaca tttagttgcc ccgagattac tgacaaagtg cgcgttgttc atgccggatg
    25921 cggcgtgacc gccttatccg gcctacgaaa ccgcaagaat tcaatgtatt gcaggagcgg
    25981 tgtaggcctg ataagcgtag cgcatcaggc agttttgcgt ttgcccacaa tcttagggga
    26041 catttagcgg ccccatttat ttctcacttt tccgcctcat catcgcgcgt taatttcttt
    26101 tcataatcgc gttttacaat atccagcgcg cgcagcacgg tgcgggctgg gatctgattt
    26161 tcttccagca gcacaatcaa atcgacagcc agtttgacat cgtcaggggc attttcaagt
    26221 gacatactct ctccattgct agcgggttaa gcgcgctagc ctgttttcga ttttttccag
    26281 cgcatggcgg cagcgtgcca ggcgcgcttc ataggcttcc acttcacgat gcagcgtttg
    26341 ctgttccacg agatcggtca cccgcgccag gcgggctctg cgttcggcaa ccatctcacg
    26401 tagccgccgc tcaaaatcct gatgctgaat acgtttacgc tgccagcggg caattttcgg
    26461 tggcgcactg tcccactcgc gcaatgacca ggcggaggct tcacgcgcga tggcttccag
    26521 ttgtgccgcc agatgttccg ccagccaggc cacttgcggc agctgttgct gctcaaccgc
    26581 atgacaaagc gcagccagat tatcgcccgc ctcgtcgaga caagcctgta gtgttgtcgc
    26641 acgagtctga aaaagatgcc tgtcgaaacg agcacttagc gtggcgaact gtgacaccgg
    26701 ggcacaacgc tgacgcagcg tagcgagctg tccttccagt ttttccagca gcagggcggt
    26761 tttcacgata agacctcaat gaaaatgata attgttatgc taaagtagcc actctgtaag
    26821 ctgacaacac tcaatatgca acgaatcatt ttaatcatca ttggctggct ggcggtagtg
    26881 ctgggtacgc tgggcgtggt attaccggta ttaccgacga cgccgtttat cctgctggcg
    26941 gcctggtgct ttgcccgttc ttccccgcgc tttcacgcct ggttgctgta ccgctcatgg
    27001 tttggcagct atctacgttt ctggcagaaa catcatgcga tgccgcgcgg cgtcaaaccg
    27061 cgggcgattt tgcttatttt gctcacgttt gccatttctc tgtggttcgt ccagatgcca
    27121 tgggtgcgca tcatgttgct ggtaattctc gcctgtttgc ttttctatat gtggcgaatt
    27181 ccggtgattg atgaaaagca agaaaagcac tgaagcacaa caatcgcagt tgcaattatt
    27241 gcgtacagcc agtacattct ggcgttttcg agcacaggcg caggcggtca aaggttaaac
    27301 aactgttact tttgatacgt ttaaaacgcg ccgtgagtac caccgtaaca agcaggcata
    27361 cacttatgac cgcgactgca cagcagcttg agtatctcaa aaatagcatc aaaagcattc
    27421 aggactaccc aaaacccggc attcttttcc gcgatgtcac cagcttactg gaagacccga
    27481 aagcttacgc tctcagcatc gacttgctgg ttgagcgtta caaaaatgcg ggcattacca
    27541 aagttgtcgg caccgaagcg cgtggcttct tgtttggcgc tccggtagct ctgggtctgg
    27601 gcgttggctt tgtaccggtc cgtaaaccgg gcaaactgcc gcgtgaaacc atcagtgaaa
    27661 cttacgacct ggaatacggc accgatcagc tggagatcca cgttgatgcc atcaaaccgg
    27721 gcgacaaagt tctggtggtg gacgatctgc tggcaaccgg cggcactatc gaagcgaccg
    27781 ttaaactgat ccgtcgtctg ggtggtgaag tggctgacgc tgcgttcatt atcaacctgt
    27841 tcgatctcgg cggcgaacag cgtctcgaaa aacagggcat taccagctac agccttgtcc
    27901 cgttcccggg ccattaatta tcgccagtct tgtgctgccc acgctacgga cagcacaaga
    27961 tgtgcattca gcctcgccgt tctgacgggg ctgtgttagc attacccctt cgtgaatcca
    28021 ccttccagcg tttcagagcc tgccaatgag ttatcaggtc ttagcccgaa aatggcgccc
    28081 acaaaccttt gctgacgtcg tcggccagga acatgtgctg accgcactgg cgaacggctt
    28141 gtcgttaggg cgtattcatc atgcttatct tttttccggc acccgtggcg tcggaaaaac
    28201 ctctatcgcc cgactgctgg cgaaggggct aaactgcgaa accggcatta ccgcgacgcc
    28261 gtgcggcgtg tgcgataact gtcgtgaaat cgagcagggg cgctttgtcg atctgattga
    28321 aatcgacgcc gcctcgcgca ccaaagttga agatacccgc gacctgctgg ataacgtcca
    28381 gtacgctccg gcgcgtggtc gtttcaaagt ttatctgatc gacgaagtgc atatgctgtc
    28441 gcgccacagc tttaacgcac tgttaaaaac ccttgaagag ccgccggagc acgttaagtt
    28501 tctgctggcg acgaccgatc cacagaaatt gccggtgacg attttgtcgc gctgtttgca
    28561 atttcatctc aaggcgctgg atgtcgagca aattcgccat caacttgagc acatcctcaa
    28621 cgaagaacat atcgctcacg agccgcgggc gttgcaattg ctggcgcgcg ccgctgaagg
    28681 cagcctgcga gatgccttaa gtctgaccga ccaggcgatt gccagcggtg acggccaggt
    28741 ttcaacccag gcagtcagtg cgatgctggg tacgcttgat gacgatcagg cgctgtcgct
    28801 ggttgaagcg atggtcgagg ccaacggcga gcgcgtaatg gcgctgatta atgaagccgc
    28861 tgcccgtggt atcgagtggg aagcgttgct ggtggaaatg ctcggcctgt tgcatcgtat
    28921 tgcgatggta caactttcgc ctgctgcact tggcaacgac atggccgcca ttgagctgcg
    28981 gatgcgtgaa ctggcgcgca ccataccgcc gacggatatt cagctttact atcagacgct
    29041 gttgattggt cgcaaagaat taccgtatgc gccggaccgc cgcatgggcg ttgagatgac
    29101 gctgctgcgc gcgctggcgt tccatccgcg tatgccgctg cctgagccag aagtgccacg
    29161 ccagtccttt gcacccgtcg tgccaacggc agtaatgacg ccaacccagg tgccgccgca
    29221 atcagcgccg cagcaggcac cgactgtacc gctcccggaa accaccagcc aggtgctggc
    29281 ggcgcgccag cagttgcagc gcgtgcaggg agcaaccaaa gcaaaaaaga gtgaatcggc
    29341 agccgctacc cgcgcgcggc cggtgaataa cgctgcgctg gaaagactgg cttcggtcac
    29401 cgatcgcgtt caggcgcgtc cggtgccatc ggcgctggaa aaagcgtcag ccaaaaaaga
    29461 agcgtatcgc tggaaggcga ccactccggt gatgcagcaa aaagaagtgg tcgccacgcc
    29521 gaaggcgctg aaaaaagcgc tggaacatga aaaaacgccg gaactggcgg cgaagctagc
    29581 ggcagaagcc attgagcgcg acccgtgggc ggcacaggtg agccaacttt cgctaccaaa
    29641 actggtcgaa caggtggcgt taaatgcctg gaaagaggag agcgacaacg cagtatgtct
    29701 gcatttgcgc tcctctcagc ggcatttgaa caaccgcggt gcacagcaaa aactggctga
    29761 agcgttgagc atgttaaaag gttcaacggt tgaactgact atcgttgaag atgataatcc
    29821 cgcggtgcgt acgccgctgg agtggcgtca ggcgatatac gaagaaaaac ttgcgcaggc
    29881 gcgcgagtcc attattgcgg ataataatat tcagaccctg cgtcggtttt tcgatgcgga
    29941 gctggatgaa gaaagtatcc gccccatttg atcgtaagca cagcttacgt tcgtcatcct
    30001 taacgtgatt gagagagaaa cctatgtttg gtaaaggcgg tctgggtaac ctgatgaagc
    30061 aagcccagca gatgcaagaa aaaatgcaga aaatgcagga agagatcgcg cagctggaag
    30121 tcaccggcga atctggcgca ggtctggtaa aagtgaccat caacggtgca cacaactgcc
    30181 gtcgcgtaga gatcgacccg agcctgctgg aagacgacaa agagatgctg gaagacctgg
    30241 tggctgcagc attcaacgac gcagcacgtc gtattgaaga aacgcagaaa gaaaaaatgg
    30301 cctctgtatc ctccggaatg cagctgccgc ctggctttaa gatgccgttc tgatgcaaac
    30361 cagcccgctg ttaacacagc ttatggaagc actgcgctgt ctgccgggcg ttggcccgaa
    30421 gtcggcgcag cgtatggcgt tcacgctgct tcagcgcgat cgtagcggcg ggatgcgttt
    30481 ggcgcaggcg ctcacccggg cgatgtcgga aatcggccac tgcgccgatt gccgcacttt
    30541 caccgaacag gaagtctgta acatctgttc gaatccgcgt cgtcaggaaa acggtcaaat
    30601 ctgtgtggtg gagagtccgg cggacatcta cgccattgag cagacggggc agttttctgg
    30661 tcgttatttt gtgttgatgg ggcatctgtc accgctggac ggcatcggtc cggatgatat
    30721 cgggcttgat cgtctggaac agcgtctggc agaggaaaaa atcactgaag tgatcctcgc
    30781 caccaatccc acggttgaag gtgaagctac cgctaactac attgccgagc tttgcgcgca
    30841 atatgacgtg gaagccagcc gaatcgctca tggcgtaccg gttggcggcg aactggaaat
    30901 ggtcgatggc accacgctgt cacactccct tgccgggcgt cataagattc gtttttaagc
    30961 aaacgagagc aggatcacct gctctcgctt gaaattattc tcccttgtcc ccatctctcc
    31021 ctcatcctgt ttttaacctt aaaatggcat tattgaggta gacctacatg aaaggacaag
    31081 aaactcgtgg ttttcagtca gaagtgaaac agcttctgca cctgatgatc cattctctct
    31141 attccaataa agaaatcttc ctgcgtgagc ttatctctaa cgcctccgat gcggcggaca
    31201 agctgcgttt ccgtgcgcta tctaacccgg acctgtacga aggtgatggc gaactgcgcg
    31261 ttcgtgtctc tttcgataaa gacaagcgca cgctgaccat ctctgataac ggcgtgggga
    31321 tgacccgcga cgaagtgatt gaccatctgg ggactatcgc taaatccggt accaaatcat
    31381 tcctcgaatc cctgggttct gaccaggcga aagacagcca gctgatcggt cagtttggtg
    31441 ttggtttcta ctctgcgttt atcgtggccg acaaagtgac cgtgcgtact cgcgcggcag
    31501 gcgaaaaacc agaaaacggc gtcttctggg aatcggctgg cgaaggtgaa tataccgttg
    31561 ccgacatcac caaagaagat cgtggtactg aaatcaccct gcatctgcgt gaaggcgaag
    31621 acgagttcct cgatgactgg cgcgtgcgtt ccatcatcag caaatactcc gaccatatcg
    31681 cgctgccggt agagatcgaa aaacgcgaag agaaagacgg cgaaaccgtt atctcctggg
    31741 agaaaatcaa caaagcgcag gcgctgtgga ctcgtaacaa gtcggaaatc accgatgaag
    31801 agtacaaaga gttctacaaa cacatcgccc acgactttaa tgatccgctg acctggagcc
    31861 acaaccgcgt tgaaggtaag caggagtaca ccagcctgct gtacattccg tcccaggctc
    31921 cgtgggatat gtggaaccgt gatcataaac acggcctgaa actgtacgtc cagcgtgtgt
    31981 tcatcatgga cgacgcagaa cagttcatgc cgaactatct gcgcttcgtg cgtggtctga
    32041 ttgactccag cgatctgccg ctgaacgttt cccgtgaaat cctccaggac agcacggtaa
    32101 cgcgtaacct gcgcaatgcg ctgaccaagc gtgtgctgca aatgctggaa aaactggcga
    32161 aagacgacgc ggaaaaatac cagaccttct ggcaacagtt tggcctggta ctgaaagaag
    32221 gtccggcgga agatttcgct aaccaggaag cgatcgccaa actgctgcgt tttgcttcta
    32281 cccataccga ttcttctgcg cagaccgtat ctctggaaga ctacgtttcc cgcatgaaag
    32341 aagggcagga gaaaatctac tacatcaccg cagacagcta tgcggcagcg aagagcagcc
    32401 cgcacctgga actgctgcgt aagaaaggca tcgaagttct gctgctttcc gaccgcatcg
    32461 atgagtggat gatgaactat ctgactgagt tcgacggtaa accgttccag tcggtgtcta
    32521 aagttgacga gtcgctggaa aaactggctg acgaagttga tgagagcgcg aaagaagcgg
    32581 agaaagcact gactccgttc atcgaccgtg tgaaagccct gctcggcgag cgcgtgaaag
    32641 atgtccgtct gactcaccgt ctgaccgata cgccagcgat cgtttcgacc gacgcggacg
    32701 aaatgagcac tcagatggcg aaactgttcg ctgcggcggg ccagaaagtg ccagaagtga
    32761 aatacatctt cgaactgaac ccggatcacg tactggtgaa acgtgcggca gatactgaag
    32821 atgaagcgaa gttcagcgag tgggtagaac tgctgctgga tcaggcgctg ctggcagaac
    32881 gcggcacgct ggaagatccg aacctgttta ttcgtcgtat gaaccagctg ctggtttcct
    32941 gatgtcatgc cggatgacct ttgtgtcatc cggcattttt cttttcatca tctgcacttt
    33001 ccgcaaatta tctcgccatt aaccgtttca gcctcaggtg cctttcttga ggcaatcgcc
    33061 tgttggtggt atcgtttatc gctttttcaa aaaaatcgac acattttaag gggattttcg
    33121 caatgcgtat cattctgctt ggcgctccgg gcgcggggaa agggactcag gctcagttca
    33181 tcatggagaa atatggtatt ccgcaaatct ccactggcga tatgctgcgt gctgcggtca
    33241 aatctggctc cgagctgggt aaacaagcaa aagacattat ggatgctggc aaactggtca
    33301 ccgacgaact ggtgatcgcg ctggttaaag agcgcattgc tcaggaagac tgccgtaatg
    33361 gtttcctgtt ggacggcttc ccgcgtacca ttccgcaggc agacgcgatg aaagaagcgg
    33421 gcatcaatgt tgattacgtt ctggaattcg acgtaccgga cgaactgatt gttgatcgta
    33481 tcgtaggccg ccgcgttcat gcgccgtctg gtcgtgttta tcacgttaaa ttcaatccgc
    33541 cgaaagtaga aggcaaagac gacgttaccg gtgaagaact gactacccgt aaagacgatc
    33601 aggaagaaac cgtgcgtaaa cgtctggttg aataccatca gatgactgca ccgctgatcg
    33661 gctactactc caaagaagcg gaagcgggta acaccaaata cgcgaaagtt gacggcacca
    33721 agtcggttgc tgaagttcgc gctgatctgg aaaaaatcct cggctaatca agccgcagca
    33781 gatctgcttt cgggcaggtc tgctcatcca cctcaggcaa tacctctcac agcaattagt
    33841 tcttcttcct cacttttccg ctacaattat caacaagttg aatcgataag aggcggtaat
    33901 gcgtcagact aaaatcggta tcctgctggc aaacctgggt acgcccgatg cccccacacc
    33961 tgaagcggta aaacgctatc tgaaacaatt tttaagcgac agacgcgtgg ttgatacctc
    34021 acggttgtta tggtggccat tgctgcgcgg cgttattttg ccgctgcgct cgccgcgtgt
    34081 ggcgaagctg tacgcctcgg tctggatgga aggtggctcg ccgctgatgg tttacagccg
    34141 ccagcaacag caggcgctgg cacaacgttt accggagacg cccgtagcgc tgggaatgag
    34201 ctacggctcg ccatcactgg aaagcgccgt cgatgaactc ctggcagagc atgtagatca
    34261 tattgtggtg ctgccgcttt atccgcaatt ctcctgttct acggtcggtg cggtatggga
    34321 tgaactggca cgcattctgg cgcgcaaacg tagcattccg gggatatcgt ttattcgtga
    34381 ttacgccgat aaccacgatt acattaatgc actggcgaac agcgtacgcg cttcttttgc
    34441 caaacatggc gaaccggatc tgctgctgct ctcttatcat ggcattcccc agcgttatgc
    34501 agatgaaggc gatgattacc cgcaacgttg ccgcacaacg actcgcgaac tggcttccgc
    34561 actggggatg gcaccggaaa aagtgatgat gacctttcag tcgcgctttg gccgggaacc
    34621 ctggctgatg ccttataccg acgaaacgct gaaaatgctc ggagaaaaag gcgtaggtca
    34681 tatacaggtg atgtgcccgg gctttgctgc ggattgtctg gagacgctgg aagagattgc
    34741 cgagcaaaac cgtgaggtct tcctcggtgc gggcgggaaa aaatatgaat atattccagc
    34801 gcttaatgcc acgccggaac atatcgaaat gatggctaat cttgttgccg cgtatcgcta
    34861 aagctgagcg gtaaagaact gagcgccgtc gcgaagagcc tcgtcggcgg ttttcatcat
    34921 ccgtgaataa tgcaaaaagg catgcagcgt acctgggtag agtttgaact cacagggctg
    34981 ctgatgcgcc gctaacgtct ggtaaagcag acggctgtca tccagcagcg gatcgaactc
    35041 cgccccggca ataaaacagg gcggaacttc gcgagtgaga tcattattaa acagacagta
    35101 atacggtgac tcgcggtccg cgtcgttgct taaatacgcc tcttcataca tctgcaaatc
    35161 ctgttgcgtt aagccatccc agacaccgcc caacagacga cgagtcacgg aatcccgtaa
    35221 tccgtaaagc ccataccaca gcaaaacacc cgcaatttta ccgcaatcga tctgtttatc
    35281 acgcaaccac aacgcactgg cgagcgccag catggcacct gcggaatcac cggcaaagcc
    35341 aatgcgggac atattgattt gataatcctc cgcctgctgg tggaaataac aacaagcagc
    35401 cacaatttcc tctatcgctt gcggaaaacg cgcttcaggt gaaagggtgt aatcaatacc
    35461 aatcaccgta cattggctgt agcttgccag caggcgcatg atgcgatcgt gggtatcgag
    35521 attgccgaga ataaaaccgc ctccatgcaa ataaaatagc gtcgccgggc tatctggctg
    35581 cggacaaaag agacgtgttt ccacctgccc atattttgtt ggaaccatgt aagctctggt
    35641 tgccatttct ggagcgcccg cattccagaa tcggcgctca agcgtgtaat actgtcgttg
    35701 ctcagcaatc gttcccgttg cgggccagga cggtaaatcc ggctgaagag tattcacaac
    35761 ggtcttcatt tcagcagaaa taaggtccag aacaggtagt ttgttttccg gcttcataca
    35821 aaaactcctt tcaaattacg tcattgtaag gaaccactgc catgaaaatg cgatcccgcc
    35881 tgctgatatt gaaactggct gcgtctcgcg cgctcccgtc agattgtgtt aacattcgcc
    35941 gctcagttaa ccacccgtaa aaacaaccat gaaatttccc ggtaaacgta aatccaaaca
    36001 ttacttccct gtaaatgcac gcgatccgct gcttcagcaa ttccagccag aaaacgaaac
    36061 cagcgccgcc tgggtcgtag gtattgacca gacgctggtc gatattgaag cgaaagtgga
    36121 tgatgaattc attgagcgtt atggattaag cgccgggcat tcactggtga ttgaggatga
    36181 cgtagccgaa gcgctttatc aggagctaaa acagaaaaac ctgattaccc atcagtttgc
    36241 gggtggcacc attggtaaca ccatgcacaa ctactcggtg ctcgcggacg accgttcggt
    36301 gctgctgggc gtcatgtgca gcaatattga aattggcagt tatgcctatc gttacctgtg
    36361 taacacttcc agccgtaccg atcttaacta tctacaaggc gtggatggcc cgattggtcg
    36421 ttgctttacg ctgattggcg agtccgggga acgtaccttt gctatcagtc caggccacat
    36481 gaaccagctg cgggctgaaa gcattccgga agatgtgatt gccggagcct cggcactggt
    36541 tctcacctct tatctggtgc gttgcaaacc gggtgaaccc atgccggaag caaccatgaa
    36601 agccattgag tacgcgaaga aatataacgt accggtggtg ctgacgctgg gcaccaagtt
    36661 tgtcattgcc gagaatccgc agtggtggca gcaattcctc aaagatcacg tctctatcct
    36721 tgcgatgaac gaagatgaag ccgaagcgtt gaccggagaa agcgatccgt tgttggcatc
    36781 tgacaaggcg ctggactggg tagatctggt gctgtgcacc gccgggccaa tcggcttgta
    36841 tatggcgggc tttaccgaag acgaagcgaa acgtaaaacc cagcatccgc tgctgccggg
    36901 cgctatagcg gaattcaacc agtatgagtt tagccgcgcc atgcgccaca aggattgcca
    36961 gaatccgctg cgtgtctatt cgcacattgc gccgtacatg ggcgggccgg aaaaaatcat
    37021 gaacactaat ggagcggggg atggcgcatt ggcagcgttg ctgcatgaca ttaccgccaa
    37081 cagctaccat cgtagcaacg taccaaactc cagcaaacat aaattcacct ggttaactta
    37141 ttcatcgtta gcgcaggtgt gtaaatatgc taaccgtgtg agctatcagg tactgaacca
    37201 gcattcacct cgtttaacgc gcggcttgcc ggagcgtgaa gacagcctgg aagagtctta
    37261 ctgggatcgt taagttatcg tcggttcgta ggccagataa ggcgttcacg ccgcatctgg
    37321 catttggctc tcgatgcctg atgcgacgct ggcgcgtctt atcatgccta catatttttc
    37381 atattttaca tccggcaacc accgtttacc ccgtcaccac ttcacccgcc ggtggcgttt
    37441 ccagcagttc cagcatagta cgggcaattt cacgttcacc catcactacc tgattcgcgc
    37501 cacgttcggt gatatacgtc acttcatcgt cataatgggc gcgggcgata atctcaatat
    37561 ccgggttttt cgcgcgggca gacgccacaa tctcacccgc ttcataaccg ttggggatcg
    37621 tcaggatcag ccattttgca cattccagat gcgccagttg cataatttct tcgttcgctg
    37681 cattgcccaa tacagcgcgg accccacgct ctcgcagctc atcaacacgg gttcgtgacg
    37741 tctcaatcac caccagcgga atatcagagg cgagcaattt ctcccccagc aggctgccta
    37801 cacgaccgta acccaccaat agcgcatggt tgcaaatatc cactgggatc tgtttctctt
    37861 cttcgattgc ctcttccagc gtctgctctt ccagcgtttc ggtcttcgcc agatatttct
    37921 ccagtagtgc gaacagtacc gggttgagca taatcgacag gatcgcccct gccagtacca
    37981 ggttttgtcc ggcctgcggc agtaaattca atgccattcc cagtcccgcc aggataaacg
    38041 cgaactcacc aatctgcgcc aggctggcgg cgatggttaa tgccgtacgt tgggagtgac
    38101 caaacagacg gacaaggaac agggccgcga ccgatttacc aaacagaata attgccagcg
    38161 tcgccagcac tgccagcggt tgctgaatca gaattaacgg atcaaacaac atcccgacgg
    38221 agacaaaaaa cagcaccgca aacgcgtcgc gcaatggcag cgtatcgtgg gcggcacggt
    38281 gactcagttc agactcgttc agtaccatcc cggcaaagaa cgcgccaagt gcgaaggaga
    38341 cgtcaaacaa ctccaccgca ccaaaggcaa tccctaacgc cagcgccagc accgacaggg
    38401 taaatagctc gcgagaaccg gttgccgcgc tgcgtgccat aatccacggc accagacggc
    38461 gacctaccag catcataatg gcgataaatg cgatcacttt gccgatggtg atccccatat
    38521 cgactgcaag agtggcaaag cccacatcgc cctgttccat cattcctgcc actgcgggca
    38581 gcaacaccag cgtcagaacc attaccaggt cttccacaat caaccaaccg atggcgattt
    38641 gcccacgctg actgtcaatt aattgccgtt cttcaagtgc gcgcagtaac accacggtac
    38701 tggcggtgga aagacataaa ccgaacacga taccggtcat taacgaccag cccagcacgg
    38761 cagagagcgc catacccagc agcgtcgcca cggctatctg ggcgatcgca ccgggaatgg
    38821 cgatggcctt taccgccatc aaatccttca gcgaaaagtg caaaccgacg ccaaacatca
    38881 acagaatgac gcccagttca gccagttccg gcgcaagctt ggtatcggca acaaagcccg
    38941 gagtgaatgg tcctgccagc acacccgcta acagatatcc caccagagga gaaatacgta
    39001 gtttattggc cagcatgccg aggataaagg cgagcacaag gccgccaaca atggtggtga
    39061 taagcggggt ggcgtgatgc attccgtctc cttttcctgg tggttattgt ccatttttgg
    39121 ccgggaaaac caaaattaca ggtaatagtt tatgacaatt tcattgatga tgttcatgaa
    39181 taattattga attttgcaga aaaatggaat tagctgcaaa aaaagcacgg atcggaaaac
    39241 agaaggcggt ttaacagagg aaaggcttat ggcgactgta aaacgatgca gccaaagttt
    39301 gcctttggct gcaatgaaat cagtctttat gccggttatc aggcaggaat atggtcaaca
    39361 tccccaatag tggcaggaaa gcacagattt tatagactaa ctcgatgct
//
LOCUS       DAKISA010000034        38337 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.034, whole genome shotgun sequence.
ACCESSION   DAKISA010000034 DAKISA010000000
VERSION     DAKISA010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38337
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.034"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            59..1423
                     /locus_tag="OVW45_001778"
     CDS             59..1423
                     /locus_tag="OVW45_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCV5526246.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSIVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="OVW45_001779"
     CDS             complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="OVW45_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCV5526247.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2210..3121
                     /gene="panE"
                     /locus_tag="OVW45_001780"
     CDS             2210..3121
                     /gene="panE"
                     /locus_tag="OVW45_001780"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCV5526248.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3084..3674
                     /gene="yajL"
                     /locus_tag="OVW45_001781"
     CDS             3084..3674
                     /gene="yajL"
                     /locus_tag="OVW45_001781"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCV5526249.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(3728..5176)
                     /gene="thiI"
                     /locus_tag="OVW45_001782"
     CDS             complement(3728..5176)
                     /gene="thiI"
                     /locus_tag="OVW45_001782"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCV5526250.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDEKQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5382..5624
                     /gene="xseB"
                     /locus_tag="OVW45_001783"
     CDS             5382..5624
                     /gene="xseB"
                     /locus_tag="OVW45_001783"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCV5526251.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLPPFTPDNE"
     gene            5624..6523
                     /gene="ispA"
                     /locus_tag="OVW45_001784"
     CDS             5624..6523
                     /gene="ispA"
                     /locus_tag="OVW45_001784"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCV5526252.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6548..8410
                     /gene="dxs"
                     /locus_tag="OVW45_001785"
     CDS             6548..8410
                     /gene="dxs"
                     /locus_tag="OVW45_001785"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCV5526253.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVTAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8465..9439
                     /gene="yajO"
                     /locus_tag="OVW45_001786"
     CDS             8465..9439
                     /gene="yajO"
                     /locus_tag="OVW45_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCV5526254.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9493..10011)
                     /gene="pgpA"
                     /locus_tag="OVW45_001787"
     CDS             complement(9493..10011)
                     /gene="pgpA"
                     /locus_tag="OVW45_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCV5526255.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(9989..10966)
                     /gene="thiL"
                     /locus_tag="OVW45_001788"
     CDS             complement(9989..10966)
                     /gene="thiL"
                     /locus_tag="OVW45_001788"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCV5526256.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11044..11463)
                     /gene="nusB"
                     /locus_tag="OVW45_001789"
     CDS             complement(11044..11463)
                     /gene="nusB"
                     /locus_tag="OVW45_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCV5526257.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11483..11953)
                     /gene="ribE"
                     /locus_tag="OVW45_001790"
     CDS             complement(11483..11953)
                     /gene="ribE"
                     /locus_tag="OVW45_001790"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCV5526258.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12042..13145)
                     /gene="ribD"
                     /locus_tag="OVW45_001791"
     CDS             complement(12042..13145)
                     /gene="ribD"
                     /locus_tag="OVW45_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCV5526259.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13149..13598)
                     /gene="nrdR"
                     /locus_tag="OVW45_001792"
     CDS             complement(13149..13598)
                     /gene="nrdR"
                     /locus_tag="OVW45_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCV5526260.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13749..14288
                     /locus_tag="OVW45_001793"
     CDS             13749..14288
                     /locus_tag="OVW45_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCV5526261.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            14587..15471
                     /gene="tsx"
                     /locus_tag="OVW45_001794"
     CDS             14587..15471
                     /gene="tsx"
                     /locus_tag="OVW45_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCV5526262.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYDAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15648..15995)
                     /locus_tag="OVW45_001795"
     CDS             complement(15648..15995)
                     /locus_tag="OVW45_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCV5526263.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16124..17095)
                     /gene="secF"
                     /locus_tag="OVW45_001796"
     CDS             complement(16124..17095)
                     /gene="secF"
                     /locus_tag="OVW45_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCV5526264.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17106..18953)
                     /gene="secD"
                     /locus_tag="OVW45_001797"
     CDS             complement(17106..18953)
                     /gene="secD"
                     /locus_tag="OVW45_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCV5526265.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(18981..19313)
                     /gene="yajC"
                     /locus_tag="OVW45_001798"
     CDS             complement(18981..19313)
                     /gene="yajC"
                     /locus_tag="OVW45_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCV5526266.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19336..20463)
                     /gene="tgt"
                     /locus_tag="OVW45_001799"
     CDS             complement(19336..20463)
                     /gene="tgt"
                     /locus_tag="OVW45_001799"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCV5526267.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20519..21589)
                     /gene="queA"
                     /locus_tag="OVW45_001800"
     CDS             complement(20519..21589)
                     /gene="queA"
                     /locus_tag="OVW45_001800"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCV5526268.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21682..22263
                     /gene="acpH"
                     /locus_tag="OVW45_001801"
     CDS             21682..22263
                     /gene="acpH"
                     /locus_tag="OVW45_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HCV5526269.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22268..24085)
                     /gene="malZ"
                     /locus_tag="OVW45_001802"
     CDS             complement(22268..24085)
                     /gene="malZ"
                     /locus_tag="OVW45_001802"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCV5526270.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKHRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLGLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGSTEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKERHGQLTDGILALPAISATVWMN"
     gene            complement(24241..25614)
                     /gene="proY"
                     /locus_tag="OVW45_001803"
     CDS             complement(24241..25614)
                     /gene="proY"
                     /locus_tag="OVW45_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCV5526271.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRCLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(25690..27009)
                     /gene="brnQ"
                     /locus_tag="OVW45_001804"
     CDS             complement(25690..27009)
                     /gene="brnQ"
                     /locus_tag="OVW45_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCV5526272.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(27416..28711)
                     /gene="phoR"
                     /locus_tag="OVW45_001805"
     CDS             complement(27416..28711)
                     /gene="phoR"
                     /locus_tag="OVW45_001805"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCV5526273.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEESGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(28769..29458)
                     /gene="phoB"
                     /locus_tag="OVW45_001806"
     CDS             complement(28769..29458)
                     /gene="phoB"
                     /locus_tag="OVW45_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCV5526274.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            29648..30850
                     /gene="sbcD"
                     /locus_tag="OVW45_001807"
     CDS             29648..30850
                     /gene="sbcD"
                     /locus_tag="OVW45_001807"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCV5526275.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            30847..33990
                     /gene="sbcC"
                     /locus_tag="OVW45_001808"
     CDS             30847..33990
                     /gene="sbcC"
                     /locus_tag="OVW45_001808"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCV5526276.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASALAQHSEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTTSLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALTGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVKQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            34116..35300
                     /gene="araJ"
                     /locus_tag="OVW45_001809"
     CDS             34116..35300
                     /gene="araJ"
                     /locus_tag="OVW45_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCV5526277.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISVFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(35443..36351)
                     /gene="mak"
                     /locus_tag="OVW45_001810"
     CDS             complement(35443..36351)
                     /gene="mak"
                     /locus_tag="OVW45_001810"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCV5526278.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            36476..37387
                     /gene="rdgC"
                     /locus_tag="OVW45_001811"
     CDS             36476..37387
                     /gene="rdgC"
                     /locus_tag="OVW45_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCV5526279.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFALRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(37465..37920)
                     /locus_tag="OVW45_001812"
     CDS             complement(37465..37920)
                     /locus_tag="OVW45_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5526280.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYLMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(37917..38252)
                     /locus_tag="OVW45_001813"
                     /pseudo
     CDS             complement(37917..38252)
                     /locus_tag="OVW45_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
BASE COUNT         9465 a        10313 c         9736 g         8823 t
ORIGIN      
        1 tgtttaccca acatgcgctc cgcactacac tatgtgggca tttttttaag gggtggtaat
       61 gaacgattat aaaatgacgc caggtgagag gcgcgcgacc tggggtttag ggaccgtatt
      121 ctcgttgcgc atgctgggca tgtttatggt tctgccggtt ctgaccacgt acggcatggc
      181 tctgcaaggt gccagcgaag cattaatcgg tattgccatt ggtatttatg gtctgactca
      241 ggccgttttt cagattccgt ttggcctgct ttccgaccgc attggtcgca aaccattaat
      301 tgtcggtggg ctggcggtgt ttgccgccgg tagcgttatc gctgcgcttt ccgactccat
      361 ctggggaatt attctgggcc gggcgctaca aggctctgga gcaattgccg ccgccgttat
      421 ggcgctgctt tccgatctca cgcgcgaaca aaaccgcacc aaagcgatgg cgtttatcgg
      481 cgtgagcttt ggcattacct ttgccattgc gatggtgctt ggcccgatca tcactcacaa
      541 acttgggctg cacgcgctgt tctggatgat cgctattctg gcaacaaccg gcattgcgtt
      601 gaccatttgg gttgtgccca acagtagcac tcacgtactt aatcgtgagt ccggaatggt
      661 gaaaggcagt ttcagtaaag tgctggcgga accgcggctg ctgaaactca actttggcat
      721 tatgtgtctg cacatgcttc tgatgtctac gtttgttgcc ctgcccggac agctagctga
      781 tgcggggttc ccggcggctg aacactggaa ggtctatctg gcgacgatgt taatcgcctt
      841 tggctcgatc gtgcctttca ttatctacgc tgaagttaag cgcaaaatga agcaagtctt
      901 tgtcttctgc gtcgggttga tcgtggttgc ggaaattgtg ttgtggaacg cacaaacgca
      961 gttctggcaa ctggtggtcg gcgtgcagct tttctttgtg gcgtttaatt tgatggaagc
     1021 ccttctgccc tcacttatca gtaaagagtc gccagcaggt tacaaaggta cggcgatggg
     1081 tgtctactcc accagccagt ttcttggcgt ggcgattggc ggttcgctgg gcggctggat
     1141 tgacggcatg tttgacggtc agggggtatt tctcgctggc gcaatgctgg ccgcagtgtg
     1201 gctggcagtc gccagtacca tgaaagaacc gccgtatgtc agcagtttgc gcattgaaat
     1261 cccggcggac attgccgcaa acgaagcgtt aaaagtgcgt ttgctggaaa ctgaaggcgt
     1321 caaagaagtg ttgattgcag aagaagagca ttcagcttat gtgaaaatcg acagcaaagt
     1381 gacaaatcgc tttgaggttg aacaggcaat tcgtcaggca taaaaaacgc ggcgaattgc
     1441 ctgctgcgct acgcttacca ggcctacaaa gttcttgagt tacaaaccgt tgtaggccga
     1501 atcaggcgtt catgccgcat ctgacatgaa caaaacgcac attgtcgcga ttaatcgcgg
     1561 aagtttttga actggaacgg ctggccgaga tcgccaccac gtaccatcgc catgacagcc
     1621 tgcaaatcat cacgagattt gccagtcaca cggatctcat cgccctgaat ttgcgcctgc
     1681 actttcagtt tgctgtcttt aatcatcttg acgattttct tctgggtcgc gctctcaatg
     1741 ccctgtttca gtttcgcttc cacaaaccag gttttaccgc tatgaacgat attttccggt
     1801 acatccagtg aactgccttc aatgccgcgc ttcagcagct tggcacgcag aatatccagc
     1861 aactgattga cctggaaatc ggactcgctc aacactttga tggttttgct ggcgtcgttc
     1921 agctcaaatg aggcttcaac attacggaag tcaaaacggg actccacttc gcggctcgcg
     1981 ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag agacaatatc gaaagatggc
     2041 atcttttcct ctcccttcat ttttgatgcg aagcataata cccgcaaagt taaataaccg
     2101 acagtcctgc accaggacgt atcccttaat tgtgcatagt cacaacagca tgcacaatta
     2161 agggatagcc ttattgaaca cccggagtgg ttgcgggtga ggaggaacaa tgaaaattac
     2221 cgtattggga tgcggtgcct tagggcaatt atggcttaca gcactttgta aacagggtca
     2281 cgaagttcag ggctggctgc gcgtaccgca accttattgt agtgtgaatc tggttgagac
     2341 tgatggttcg atatttaacg aatcgctgac cgccaacgat cccgattttc ttgccaccag
     2401 cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc gatgccgtca aaagcctcgc
     2461 gtccacactg cctgtaacca cgccaatact gttaattcac aacggcatgg gcaccatcga
     2521 agagttgcaa aacattcagc agccattact gatgggcacc accacccatg ccgcccgccg
     2581 cgacggcaat gtcattattc atgtggcaaa cggaatcacg catattggtc cggcacggca
     2641 acaggacggc gattacagct atctggcgga tattttgcaa accgtgttgc ctgacgttgc
     2701 ctggcataac aatattcgcg ccgagctgtg gcgcaagctg gccgtcaact gtgtgattaa
     2761 tccactgacc gccatctgga attgcccgaa tggtgaatta cgtcatcatc cgcaagaaat
     2821 tatgcagata tgcgaagaag tcgcggcagt gatcgagcgc gaagggcatc atacttcagc
     2881 agaagatttg cgtgattacg tgatgcaggt gattgatgcc acagcggaaa atatctcgtc
     2941 gatgttgcag gatatccgcg cgctgcgcca caccgaaatc gactatatca atggttttct
     3001 cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa aacacccgcc tgtttgaaat
     3061 ggtaaaaaga aaggagagtg aatatgagcg catcggcact ggtttgcctc gcccctggta
     3121 gtgaagagac tgaagccgtc accactatcg atctgctggt tcgcggcggt atcaaagtca
     3181 ccactgccag cgtcgccagc gatggcaacc tggccattac ctgctcgcgc ggcgtgaagc
     3241 tgctggcgga tgcgccgctg gtcgaggtgg ctgatggcga atatgacgtg atcgtactgc
     3301 ctggtggcat taaaggcgca gagtgttttc gcgatagtac cctgctggtt gaaaccgtta
     3361 aacagttcca ccgttccggg cgtatcgtcg cggctatttg cgccgcgcca gccaccgtgc
     3421 tggtaccgca cgatatcttc ccgattggca atatgaccgg cttcccgacg ctgaaagaca
     3481 aaattcccgc cgaacaatgg caggacaagc gcgtcgtctg ggatgcacgg gtaaaattgc
     3541 tgaccagcca ggggccgggt acggctattg attttggtct gaaaattatc gacctgctgg
     3601 ttgggcgcga gaaagcccat gaagtggcat cacaactggt gatggcggca gggatttata
     3661 attattacga gtagtgtcgg atgcggcaaa cgtcgcatct gaccagatgc gacgtaaaaa
     3721 ccacaaatta tgggcgatac accttcacat tgttaaagcc ctgctcgcgc agatagagcg
     3781 cctgcagacg gctcatcacc ccgcgctcac accacagtag ccaggttctg ttctggtcga
     3841 gatcgccaaa tttggtgctc agtttataga acggcagaga aaccacgtcg ataccttcaa
     3901 ccttcagcgg cttatcttcc tgctcatcga cagaacggat atcgaggatc acgtcgttcg
     3961 ggccaaagcc attgacggtt tccacttcca ccacttcctg ctcggtctgc tgggcgattt
     4021 cgcggatatc aacgttattc gcttcctcaa ccactttatc gagaatactg aagtcgaact
     4081 tctcttcttc cgcttcaatc ttcgatttaa ccgctttcac cgtcgggctt ttggagatca
     4141 cgccacaata ttctggcatc gtgcgagcaa agtcttcagt gccaatctgg cgggccaggt
     4201 tgatgatgtg ctctttgtcg taagaaatca gcggacgcag gatcagcgta tcagagacgt
     4261 tatcaatcag gcgcaggttg gtcagcgtct gactggacac ctggccgagc gcttcgccgg
     4321 tgaccagcgc ctgtacgccg taacgttcag ccactttaga cgcggcacgc accatcatac
     4381 gtttgaggat aacgcccatc tgaccgtcgt cgattttctc gagaatttcc ccgacgaccg
     4441 gttcgaaatt aatggcgaca aaacgcacgc ggtgggagct gccaaagcgg ttccacagat
     4501 aatgcgccac ctgacgcacg ccaatttcat gcgctgcgcc gccgaggtta aagaagcagt
     4561 aatgcacgcg gcagccgcga cgcatcaaca tataactgga aacaccggag tcgaaaccac
     4621 cggaaatgag cgacagcaca tcttcctggg tgccaatcgg gaaaccgcca ataccttcgt
     4681 agcggccttt aatcagcaga agacgatcgt cttccacttc cagatggaca gttacatccg
     4741 ggttagtcag cttcacgcgc gcggattcaa tatgctgatt taaaccgccg ccgacgtaac
     4801 gttccacatc aatcgagcta aaatcatgtt tgccacggcg cttcacgcgt acgcagaagg
     4861 ttttgccttc cagctgatcg cgatattgaa ccaacgcttt ctcgaaaata tcgtgcatgt
     4921 cggtaaacgg cacgtcttcg acttcgagaa tatggtggat acccggaata cgggtcaggg
     4981 cgtcgcgaat agccagacgc tgtttttcat cttttgcgcg aacttcgatg ttatcccagt
     5041 ggcggacgac agcgagcgtc tcatcatagt gctttaaaac gttacgaatg ttcccggtaa
     5101 ggatttttat aaagcgcaag cgcacagatt ggcttttgat ggtgatttcc gggaacaatt
     5161 taatgataaa cttcatggcg gcaatggttc gttggcaagc cttaagcgac ttgtataggg
     5221 aaaaatacag cagcccacac ctgcggctgc atccaggcgc ggaagtatac cactaacatc
     5281 gctttgctgc gcacatcacc ttaccattgc gcgttatttg ctatttgccc tgagtccgtt
     5341 accatgacgg ggcgaaaaat attgagagtc agacattcat tatgccgaag aaaaatgagg
     5401 cgcccgccag ctttgaaaag gcgctgagcg agctggaaca gattgtaacc cgtctggaaa
     5461 gtggcgacct gccgctggaa gaggcgctga acgagttcga acgcggcgtg cagctggcac
     5521 gtcaggggca ggccaaatta caacaagccg aacagcgcgt acaaattctg ctgtctgaca
     5581 atgaagacgc ctctctaccc ccttttacac cggacaatga gtaatggact ttccgcagca
     5641 acttgaagcc tgcgttaagc aggccaacca ggcgctgagc cgttttatcg ccccactgcc
     5701 ctttcagaac actcccgtgg tcgaaaccat gcagtatggc gcattattag gtggtaagcg
     5761 cctgcgacct tttctggttt atgccaccgg tcatatgttc ggcgttagca caaacacgct
     5821 ggacgcaccc gctgccgccg ttgagtgtat ccacgcttac tcattaattc atgatgattt
     5881 accggcgatg gatgatgacg atctgcgtcg cggtttgccg acctgccatg tgaagtttgg
     5941 cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg ctggcgttct cgattttaag
     6001 cgatgccgat atgccggaag tgtcggaccg cgacagaatt tcgatgattt cagaactggc
     6061 aagcgccagc ggtattgccg gaatgtgcgg tggtcaggca ttagatttag atgcggaagg
     6121 caaacacgta cctctggacg cactcgagcg tattcatcgt cataaaaccg gcgcattgat
     6181 tcgcgccgcc gttcgccttg gtgcattaag cgccggagat aaagggcgtc gtgctctgcc
     6241 agtactcgac aagtacgcag agagcatcgg ccttgccttc caggttcagg atgacatcct
     6301 ggatgtggtg ggagatactg caacgttggg aaaacgccag ggtgccgacc agcaacttgg
     6361 taaaagtacc taccctgcac ttctgggtct tgagcaagcc cggaagaaag cccgggatct
     6421 gatcgacgat gcccgccagt cgctgaaaca actggctgaa caatcactcg atacctcggc
     6481 actggaagcg ctagcggact acatcatcca gcgtaataaa taaacaataa gtattaatag
     6541 gcccctgatg agttttgata ttgccaaata cccgaccctg gcactggtcg actccaccca
     6601 ggagttacga ctgttgccga aagagagttt accgaaactc tgcgacgaac tgcgccgcta
     6661 tttactcgac agcgtgagcc gttccagcgg gcacttcgcc tccgggctgg gcacggtcga
     6721 actgaccgtg gcgctgcact atgtctacaa caccccgttt gaccaattga tttgggatgt
     6781 ggggcatcag gcttatccgc ataaaatttt gaccggacgc cgcgacaaaa tcggcaccat
     6841 ccgtcagaaa ggcggtctgc atccgttccc gtggcgcggc gaaagcgaat atgacgtatt
     6901 aagcgtcggg cattcatcaa cctccatcag tgccggaatt ggtattgcgg ttactgccga
     6961 gaaagaaggc aaaaatcgcc gcaccgtctg tgtcattggc gatggcgcga ttaccgctgg
     7021 catggcgttt gaagcgatga atcacgcggg cgatatccgt cctgatatgc tggtggtcct
     7081 caacgacaat gaaatgtcga tttccgaaaa tgtcggcgcg ctcaataacc atctggcaca
     7141 gctgctttcc ggtaagcttt actcttcgct gcgcgaaggc gggaaaaaag ttttctctgg
     7201 cgttccgcca attaaagagc tgctcaaacg taccgaagaa catattaaag gcatggtagt
     7261 gcctggcacg ttgtttgaag agctgggctt taactacatc ggcccggttg acggtcacga
     7321 tgtgctgggg cttatcacca cgctgaagaa catgcgcgac ctgaaaggcc cgcagttcct
     7381 gcatatcatg accaaaaaag gtcgtggtta tgaaccggca gaaaaagacc ccatcacttt
     7441 ccacgccgtg cctaaatttg atccctccag cggttgtttg ccgaaaagta gcggcggttt
     7501 gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa acggcagcga aagacaacaa
     7561 gctgatggcg attactccgg cgatgcgtga aggttccggc atggtcgagt tttcacgtaa
     7621 attcccggat cgttacttcg acgtggcaat cgccgagcaa cacgcggtga cctttgccgc
     7681 cggtctggcg attggtgggt acaaacccat tgtcgcgatt tactccactt tcctgcaacg
     7741 cgcctatgat caggtgctgc atgacgtggc gattcaaaag ctcccggtcc tgttcgccat
     7801 cgaccgcgcg ggcattgttg gtgctgacgg tcaaacccat cagggcgctt ttgacctctc
     7861 ttacctgcgc tgcataccgg aaatggtcat tatgaccccg agcgatgaaa acgaatgtcg
     7921 ccagatgctc tataccggct atcactataa cgacggcccg tccgcggtgc gctacccgcg
     7981 cggtaacgcg gttggcgtgg aactgacgcc gctggaaaaa ctgccaattg gcaaaggcat
     8041 tgtgaagcgt cgtggcgaga aactggcgat ccttaacttt ggtacgctga tgccagacgc
     8101 ggcgaaagtc gctgaatcgc tgaacgctac gctggtcgat atgcgttttg tgaaaccgct
     8161 tgatgaagcg ttaattctgg aaatggccgc cagccatgaa gcgctggtca ccgtagaaga
     8221 aaacgccatt atgggcggcg caggcagcgg cgtgaacgaa gtgctaatgg cccatcgtaa
     8281 accagtaccc gtgctgaaca ttggcctgcc tgacttcttt attccacaag gaactcagga
     8341 agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg gaagccaaaa tcaaggcctg
     8401 gctggcataa tccctactcc actcctgcta tgcttaagaa attattcatg gcaggagtgg
     8461 cagcatgcaa tacaacccct taggaaaaac cgaccttcgc gtttcccgac tttgcctcgg
     8521 ctgtatgacc tttggcgagc cagatcgtgg taatcacgca tggacactgc cggaagagag
     8581 tagccgtccc atcattaaac gcgcgctgga aggcggcata aatttctttg ataccgccaa
     8641 tagctattcc gatggcagca gcgaagagat cgttggtcgc gcactgcggg atttcgcccg
     8701 tcgtgaagac gtggtcgttg cgacgaaagt gttccatcgc gttggtgatt taccggaagg
     8761 attatcccgt gcacaaattt tgcgctctat cgacgacagc ctgcgccgtc tcggcatgga
     8821 ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac acgccgatcg aagagacgct
     8881 ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt tatatcggcg cgtcatccat
     8941 gcacgcttcg cagtttgctc aggccctgaa gctacaaaaa cagcacggct gggcgcagtt
     9001 tatcagtatg caggatcact acaatctgat ttatcgcgaa gaagagcgcg agatgctgcc
     9061 tctgtgttat caggagggcg tggcggtgat tccgtggagc ccgctggcgc gggggcgact
     9121 gacgcgtccg tggggagaaa ctaccgcacg actggtgtct gatgaggtgg ggaaaaatct
     9181 ctataaagaa agcgatgaaa atgacgcgca gatcgcagag cggttaacgg gcgtcagtga
     9241 agaactcggt gcaacacgag cacaagttgc gctggcctgg ttattgagta aaccgggcat
     9301 tgccgcaccg attatcggta catcgcggga agaacagctt gatgagctgc tgaacgcggt
     9361 ggatatcact ttgaagccag aacagattgc cgaactggaa acgccgtata aaccgcatgc
     9421 ggtcgtagga tttaaataag agtccaggcc tgatgagacg tgacaagcgt cacatcaggc
     9481 atcggtgcac aactacgaca gaatacccag cggccagtga tgaccgataa agtacaggat
     9541 gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg atccccatgc cgccatgtac
     9601 attgcgatca aaccagcgga tcggccacgg cttccacata tccagaatac ggaaaatcac
     9661 aaacccggcg gcaacccact gccagtcatt ggtcggcagc gccatgagcg tgatccacat
     9721 accaataaat tcgtcccaga caatgctgcc gtgatcgtgc acgcccatat ctttcgccgt
     9781 ttgatgacaa agatagacgc cgatacagat ccccaacatc accaccagcg agtagagctg
     9841 ccagggtaaa aaggtcatca gataccagaa cggaatcgct gccagcgatc ccatcgtgcc
     9901 aggaacgatt gggcttaatc cacttccgaa tccgacagca agcagatgcc acggattact
     9961 catcttcagg cgacttttcg cgacatcttt atggcgtggc aaaatggtca tatcctttcc
    10021 agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag cccttcgata tcggcggtca
    10081 tttgcccgat acaggtaaac ggtacgccaa ggtgtccgag agcaacatcc agcgcgccac
    10141 ggttcagttc cggcaccgtg aaacacaact cgtaatcttc accgccagag agcgcccagc
    10201 gcagcgcctg ttccggttca acatggcgag aaagcgcatc agaaaacggc agcaatgcca
    10261 ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg cccgagatcg gaaatcagac
    10321 cgtcagagag atcgatagct gaatttgcca gatcgcgcag tgcctgcccc tgtaaaatac
    10381 gcggcgatgg acggagatga cgtttgatca agtagtcagc atctttagca tcggcaacct
    10441 gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc gcccggtgta ccggtcacat
    10501 agatccagtc acccggtttc gccccagagc gcgttaaggc tcgtcccatc ggaacaaagc
    10561 cgtggatacc caacgtcatt gataatggcc cacgcgtggt atcgccgcca atgagttgca
    10621 tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa ggactcaagc cacgcttcgt
    10681 ctacgtccgg taaggttaat gccagcgtca gccaggccgg atcggccccc atcgctgcca
    10741 gatcgcttag gttcaccgcc agtgctttat aagccagatc agcaggatcg atatcaggga
    10801 ggaaatggtt acccgccacc agcgtatcag tgctgatcgc cagggtctgt ttctcgggga
    10861 tattgagaag tgcgcaatcg tcgccgatgc ccagttcgac atcaagacga gaacttctta
    10921 cacggtcaaa ataacgggca atcagggaga actcgccaca tgccatacgt tatgcctcag
    10981 caggtaaaaa agaaaaggcc gacgatcatg gaacggtctt ccgtgaatct accggcctgg
    11041 atatcacttt ttgttagggc gaatcacagg tgctgcttta tcgagtacgc cgttgacgaa
    11101 cttatggctg tcttctgcgc cgaacgattt cgccagttcg atcgcttcgt taatggccac
    11161 tttgtatggc acatcgctac gtttagacag ttcgtacagc gcaatgcgca gtactgcttt
    11221 ttctacctgt cccagttctt ccagcaggcg ggacaggtat ggcttcatca gtccgtcgag
    11281 gtatgcggta ttagtcgcca ccccggccag cagctcacgg aagtacagga cgtcaacgtc
    11341 ttttacatcc tgttcagcca ggaactggta ttcaacatca gcgatgtcgt tctgggacaa
    11401 ctgccaggag tagagcgcct ggacggcaca ctcacgagcg cggcgacgag cagcaggttt
    11461 cacggatttc cccttactaa tttcaggcct tgatggcttt caatacatta atcatttcaa
    11521 gcgcggtcag tgcagcttct gcacctttgt tgccagcttt ggtgccagca cgttcgatcg
    11581 cttgttcaat gctttcagtg gtcagaaccc caaaagcaac cggaatttcg ctgtcctggg
    11641 caacatgcgc caggccgttg cttgcaccac cagcgacata ttcaaagtgg gcagtgccac
    11701 cacgaataac cgtacccagc gcaatcaccg cgtcgtattt accggtttta gccagtgcac
    11761 ccgccgccag cggcagctca taggcaccag gcacccaaac aacggtaatg ttttcatctt
    11821 ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc cagcaggctg tcattgataa
    11881 agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc cggggtagca acgttagctt
    11941 caataatgtt cattttcttc cttcgggttc gagtatggcc ccgcaggggg gcggatttta
    12001 gcataatatt tcgtgcgctg cttccctttc gagccgggag atcatgcacc cactaaatgc
    12061 aggcaaacat ccgggcctac atgacgtatc tctttgaatt taaattgggg ggcgtcggct
    12121 aatttctcaa gccctggcag cgtgcataat ccgcgggcgt cgctgcctaa tagtttaggt
    12181 gcgatataga caatcagctc atcgactaaa cccgcctgca gcaatgcgcc agcgagcgtt
    12241 ggccccgctt ccacccagat gctgttaatt tgctgtttac ccagttgcat catcagtaca
    12301 accagatcca gatgaccttt atgctctgga atcagcaagg tacgcaccgt ttccggccac
    12361 tcactagaat cttcctgcgt acgcgcgaac caggtttcgc cgggctgctg cacaatgcga
    12421 tgttccggcg tcacgcgatt ttggctatca atcacaatac gtactggctg gcggagattt
    12481 tgttgcgggt agagcgcctg agtttgttca tccagttcag accaacgcac tgttaaggct
    12541 ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg catgactttg tgcgcgctgt
    12601 cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact ggctttcgcc gctcgccatc
    12661 gccgtgcggc catcaagcga tgcgccaagt tttaactgaa tataaggaaa gccggtgcgc
    12721 atccgcttga gaaagccttt attcaattgc tcggcttcac tcatcatcag cccgtggctg
    12781 acgtcaatgc cagcctgttg cagacggtaa agtccacgcc cagcgacctg cgggttagga
    12841 tcctgcatcg ctgcgaccac gcgcgctacg ccagcggcga tgagtgcgtc acagcacggt
    12901 ggcgtacggc catgatggct acagggttcg agtgtcacat acgccgttgc gcctttggct
    12961 ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg gttcacccgc tcgttggtgg
    13021 taaccttcac cgacaatttc gccatctttg acaatgacgc acccgacatt cgggttgggg
    13081 tgggtggtaa aacgtcctcg ttgcgccagc tttagcgccc gcgccatgta atactcgtcc
    13141 tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa attctttgat atcttcgaaa
    13201 ctgcggtaga cagaggcaaa acggatatag gcgactttat cgagcttttt caattgctcc
    13261 atcaccagat tgccaatcat cttgctcggt acttcgcgct caccggtggc gcgcagctgc
    13321 gatttaatat gattgattgc catttcaacg tcatcggaac tcaccggacg tttttccagc
    13381 gcccgcagca ttccgctacg caatttctct tcattaaacg gttcacgcac gtcgttgctt
    13441 tttacaacac gcggcataac cagctccgcc acttcaaagg tggtgaaacg ttcattacac
    13501 accagacact gccgacggcg gcgtacggat gaaccctcgc ccacgagacg agagtcaatt
    13561 accttagtgt ccacggcgaa acagaatggg caatgcatac ggcgtcctga ccaggtggtt
    13621 aacggaaatc tattttaccc tgaactgacg atacaacaaa ggcgcaacgc ttttgagata
    13681 aaggatcgat gctttcgctg cttttaccct ctaccattag gctaattgcg acgagaaagg
    13741 gacgacctat gaatactaac gtttttcgac tgctcctgct gggaagcctg ttcagcctta
    13801 gcgcctgtgt gcagcaaagt gaagtgcgac agatgaaaca cagcgtcagc acgctgaacc
    13861 aggagatgac gcaactcaat caagaaacag tcaaaatcac tcagcaaaac aggctgaatg
    13921 caaaatccag cagtggggtt taccttctgc ctggagcgaa aacaccggca agactggaaa
    13981 gccagatcgg tactttacgt atgtcgctgg tgaatattac gcctgataca gatggcacca
    14041 cccttacact acgtattcag ggtgagtcga atgatccctt gcccgcattc agcggaactg
    14101 ttgaatatgg gcaaattcag ggaacaatag acaactttca ggaaatcaat gtgcagaatc
    14161 aattgattaa tgcccctgcc agcgtcctcg cccccagcga tgttgatatt ccgttacaat
    14221 taaaggggat ttctgtggat cagttaggct ttgtacgtat ccacgacatt caacctgtta
    14281 tgcagtaaac gtatttcggg acgattttgt gcgtcccgca acatctttcc ccgtcatttt
    14341 gttactctgc ttacatcacc tggattgata gtaaaagttt gcaacaaggg cgaaagtcag
    14401 tacaatcccc gcccgaatgt gtgtaaacgt gaacgcaatc gattacgtaa atgatagaac
    14461 tgtgaaacga aacatatttt tgtgagcaat gatttttata ataggctcct ctgtatacga
    14521 aatatttaga aacgcaattt gcgccttttt cactcccgca agggattttc aaacagtggc
    14581 atacatatga aaaaaacatt actggcagcc ggtgcggtac tggcgctctc ttcgtctttt
    14641 actgtcaacg cagctgaaaa cgacaaaccg cagtatcttt ccgactggtg gcaccagagc
    14701 gttaacgttg tcggaagcta tcacacccgt ttcggaccgc agatccgcaa cgatacctac
    14761 cttgagtacg aagcattcgc taaaaaagac tggttcgact tctatggtta tgcggatgcg
    14821 ccggtattct tcggcggtaa ctccgatgct aaaggtatct ggaaccacgg ttctccgctg
    14881 tttatggaaa tcgaaccacg tttctccatc gacaagctga ccaatactga ccttagcttc
    14941 ggtccgttca aagagtggta cttcgcgaac aactacattt acgacatggg tcgtaataaa
    15001 gatggtcgcc agagcacctg gtacatgggt ctgggtaccg acatcgatac tggtctgccg
    15061 atgagcctgt ccatgaacgt ctatgcgaaa taccagtggc agaactatga cgcagcgaac
    15121 gaaaacgagt gggacggtta ccgtttcaaa attaaatact ttgtgccgat taccgatctg
    15181 tggggcggtc agctgagcta catcggcttc accaacttcg actggggttc cgatttaggg
    15241 gatgacagcg gtaacgcaat caacggtatt aagacccgta ctaataactc tatcgcttcc
    15301 agccatattc tggctctgaa ctacgatcac tggcactact ctgtcgtagc tcgttactgg
    15361 cacgacggtg gtcagtggaa cgacgatgca gaactgaact tcggcaacgg caacttcaac
    15421 gttcgctcta ccggctgggg tggttacctg gtagtaggtt acaacttctg attatgaaaa
    15481 tgccgggatt tattcccggc atttctgatt gttaacaacg tgtgctttgt tcatgccgga
    15541 tgcggcgtaa acgctttgtc cggcccacaa aagcacgtaa attcaatata ttgctgaaat
    15601 aacgtaggcc tgataagcgt agcgcatcag gcaattttac gttttaatca cttcttcttg
    15661 ttcatcatcg ctttcaggtc agcgaatggg ttgtacttcg cttcaccgac atctttctgc
    15721 gcatcttccc ctgcgataac ggtcgtacca tactgatccg cttcggtata tttcgaatgc
    15781 tcatgatcgt ggcaatagag acaaagcaat tcccagttac tgccatcttc cgggttattg
    15841 gtatggtcgt gatcaatgtg gtgaacggta agttcacgca ggttggaata aacaaactcg
    15901 cgggaacagc gaccgcagac ccacggatag atttttaatg ctttttcgcg atagccgctt
    15961 tctaaccgcg catagttttt tgggatgata gccatgaaaa gttacctgga tgaaatgtag
    16021 ggttacgcgc agtttcccaa gatgcgggga ttttcgcaat ctccatacat caaaaaaaat
    16081 cccgatcttc tgaccgggat tttcaacatc aacgggaact tgattacggc agaattgacg
    16141 gctgatccgc cccttctttt tccactttct gctgcaacat gtgttcgcgc ttcataccca
    16201 gtttcagagc caacgcagat gctacgtaga tggaagatgc agtgccgatg gaaacaccga
    16261 taagcatggt cagcgagaag ccttccagta ccggaccacc gaagagatac agcatcagga
    16321 taaccatcaa ggtagtaccg gatgtgatca aggtacggtg cagcgtctgg gtcaaggaca
    16381 cgttaaagat ttcgtaaggc gtaccgcgac ggatcttgcg gaagttttca cgaattcggt
    16441 ccgataccac gatactgtcg ttaagcgagt aaccgataac cgacatcaac gatgccacaa
    16501 tggtcaggtc aatctcgata tggaataacg acaaaatacc cagcgtaatg ataacgtcgt
    16561 gcgccagcgc aataaccacc cctgccgcca gtcgccactc aaagcggaaa cctacgtaca
    16621 cgaggataga cagcagcgct gccatcaacg ccatcgcacc ggtttgcgca aggtctgccc
    16681 ccacgctcgg accgacgaac tcaatacgct tcactgctgc attctgattg gtggattcgt
    16741 taatcacctt cagaacctgg ctgcccagca cctgaccgcc ggtttcgcct tcagcaggcg
    16801 gcatacggac catgatgtcg tggctgctac caaagttttg cagcattggc tcttcaaaac
    16861 cggctttttg caatgcatca cgcattacgt caatttcagc cggtttttcg agcgtaattt
    16921 cgataaccgt accaccggtg aaatccagcc cccagttaaa gccgcgcacg cccataataa
    16981 cgatagcagc gattaacagc agaccagaga tgccgaaagc ccagtagtcc cagcgcataa
    17041 agtcatagac tttacggccg tggtttagtt gttcaacagt atattcctgt gccacatcgc
    17101 actcctcaga ttgacagctt cttgacgcgc ttgccgccat atagcaggtt tacgatggca
    17161 cgcgtaccga caatcgcggt aaacatcgac gtcgccacac cgataccggt agtaatcgcg
    17221 aaccctttaa ttgccccggt acccactgcg tacaggatga tgactttaat cagcgtggtg
    17281 atgttcgcat cgaagataga actgaatgcg ccacgataac cttcatcaat tgcctgttga
    17341 acagtacgtc cgttgctcaa ctcttcttta atacgttcgt tgatcagtac gttcgcatcg
    17401 accgctaccg caagggttaa gacgataccc gcgatacctg gcatactcag tgttgcgcct
    17461 ggcaacagcg acataatacc gacgatcaag atcaagttgg caatcagagc actggtcgca
    17521 atcagaccaa acttcttata gaagatgatc atgaacagaa tagaaaccag cagaccggca
    17581 aggcaagcct ccagcccctg ctcaatgttc tgcataccca gggtcgggcc aattgtacgt
    17641 tcttcaacaa tctgaatcgg cgcgatcaac gcaccggcac gcagcagcag tgacagctga
    17701 cgggcttcgt tcgggttgtt gatgccggtg atacggaagc tgttacccag acgagactgg
    17761 atgttggcga tgttaatcac ctcttcctgt ttcaccagaa ccgcacgacc atttgcatct
    17821 ttcttaccgc tgtctttgta ctccacaaac agggttgcca tcggtttgcc gatgttgtcc
    17881 ttggtgaagt tagacataat gttgccacca gcgctatcga gcgagatgtt aacctgcggc
    17941 tggttgtatt cgtcctggct ggaagtggag tcggtgatat ggtcaccggt cagaattacg
    18001 cgtttgtaca gcacaaccgg ctgaccttcg cgggtctgtt tcacttcaga gtcgcccggt
    18061 acgcgaccgg atgccgcagc ggcctgatca acattggtgt ttaccagacg gaattccagc
    18121 gttgccgtcg cgcccagaat ctctttcgca cgcgcagtgt cctgaatacc aggcagttca
    18181 acaacgatac ggtcagcacc ctgacgctga accaccggct ccgccacgcc aagttggttt
    18241 acacggttac gcaggatatt aatgttctgc tgcaccgcat attcacgcgc ttcactcaga
    18301 cgagcatcgc tcattaccgc acgcagctgg ttgctgccct ggctgctaat caccaggtcc
    18361 ggatggcgct tgctcagata cgcaatggct tcatcacgag ctttagcatc gcggaaagtg
    18421 atgctcaggc cgtagttgtt ttctttacga acagtggtat acgggatgcc cttttcgcgc
    18481 aggtcactgc gtaggctatc gatattttgt tcctggagtt tgccaagcgc ggtgtccata
    18541 tccacttcca tcaggaagtg aacgccgcca cgcaggtcaa ggccgagctt catcggctca
    18601 gcatgaatag ctgccagcca gcgcggcgtt gccggggcaa ggttaagcgc cacgacgtat
    18661 ttgtcaccca taacgcccat taatgcttca cgagcgcgca actgggtgtc agtggagtcg
    18721 aagcgcgcaa gaatagcgcc ctcttccagt gccacagact tagcagttat tttttcttct
    18781 tgtaacgttt tctggacctg gatcagcgtt tgctcactgg cggcgactcc gcgcgcacca
    18841 gtgatctgaa cagccggatc ctcaccaaac aggttgggaa gcgcatacag cagaccgatg
    18901 acaatcacca cgatcagcat gacgtacttc cacaaaggat aacggtttaa cacggcaatt
    18961 cccttaggga aaaattttaa ttacagcgcc ttcatggtgc ctttcggcag gacggcagct
    19021 acgaagtcac gtttaataac tacttcagtg gtgtcattca gcgcgatagc aatgtagccg
    19081 ttttccgcta ctttggttac gcgaccaacc aggccaccgt tcgtcagaac ttcatcacct
    19141 ttggcaatgg agtccatcag ctttttgtgt tctttggtgc gcttctgctg tggacgcagg
    19201 atcatgaaat agaaaatcag accgaatacc accagcatca aaatcaaaga catcgggcta
    19261 ccttgcgccg gtgcacccgt tgccgctacc gcatcagaaa taaaaaagct cattaaattt
    19321 ccctcattat taatattaat caacgttcaa aggtggtact tctcgcccct gacgctggta
    19381 aaaatcagtt acgaagctct ctaatttacc ctcttcaata gccttgcgta aacccgccat
    19441 caaacgctgg tagtaacgaa ggttatgaat ggtgttgagt cgcgcgccta atatttcgtt
    19501 gcaacggtca agatgatgca agtaagcgcg tgaataattg cgacaggtgt agcaatcaca
    19561 ctcaggatcg agtgggccag tatcgctctt atacttcgca ttgcggattt tcaccacgcc
    19621 atcggtcacg aacaaatgac catttcgggc gttgcgggtt ggcattacgc agtcaaacat
    19681 atcgatacca cgacgtacgc cttcaaccag gtcttctggt ttaccaacgc ccatcaggta
    19741 acgcggtttg tctgccggaa tttgcgggca tacatgctcc agaatgcggt gcatatctgc
    19801 tttcggctca cccacagcca gaccgccgac agcgtagcca tcaaaaccga tatctaccag
    19861 acctttaaca gaaatatcac gtaaatcttc gtaaacgctg ccctgaatga taccaaacag
    19921 cgcatttttg tttccgagac tgtcaaaacg ctcacggcta cgcttcgccc aacgcagaga
    19981 catctccatg gagcgttttg cgtaatccca gtcagcagga tacggcgtac actcatcaaa
    20041 gatcatgacg atatccgaac caagatcgta ctgaatctcc attgattttt caggatcgag
    20101 gaaaatcgga tcgccgttga tcgggttacg gaagtgcacg ccctgttcgg tgattttacg
    20161 aatatcgcca aggctgaaga cctggaagcc gccggagtcg gtgaggatcg gccccttcca
    20221 ctgcataaaa tcgtgcagat cgccgtgcag tttcatgatt tcctggcccg ggcgcagcca
    20281 caggtggaag gtgttgccga ggataatttg cgcgccagtg gcttcaactt cttccggcgt
    20341 catccctttt acggtgccgt aggtgccaac aggcataaaa caaggcgttt ccactacgcc
    20401 acgatcaaag accaggcggc cacggcgtgc gcgaccgtcg gtggtgtcca gttcaaattt
    20461 cattttttct ccactacgtc agaaaaacag tccaacgttt taaaccagcg ccacggaatt
    20521 actccccgac gcgctcatta attgcctgcg gattgtacgt gataaacatc gcatcaccgt
    20581 aactaaaaaa gcgatatttc tcttctaccg ctgctttata ggcgttcatg gtgtgttgat
    20641 aaccggcaaa ggccgaaacc agcataatca gcgtcgattc cggcaggtgg aagtttgtca
    20701 ccagcgcatc gaccactttg tactggaagc ccggatagat aaagatttgg gtatcgtcaa
    20761 agaacggttc gataagatcg tttttcgccg cctgagccgc gctttccagc gaacgtactg
    20821 aggtggtgcc aaccgcaatc acccggttac cgcgcgcttt cgccgccagt accgcgtcta
    20881 ccacatcctg cggcacttca gcgtattccg agtgcatgat gtgatcttca atggtgtcga
    20941 cgcgcaccgg ctggaaggtg cccgcaccaa cgtgcaacgt cacaaacgcc atctccacgc
    21001 ctttggcgcg taatttttcc agcaaaggtt cgtcaaaatg cagacctgcg gtcggggctg
    21061 caaccgcgcc cggtttttcg ctataaacgg tttgataaag ttcgcggtca gcgtcttcgt
    21121 ccggacggtc gatatacggc ggcagcggca tatggccgat gctgttgaga atatccagca
    21181 ccgagcgttc atcattaaat tcgacttcaa acagtgcgcc gtggcgcgcg gtcattgttg
    21241 cgttaatact ttcgtcatcg cccagcagca gttctgcgcc aggttttggc gctttcgagg
    21301 cgcgaatatg cgcaagaatg cgtttgtcgt cgagcatccg ttcaaccagc acttcaatct
    21361 tgccgccgct ggctttacgc ccaaacaggc gcgccgggat cacgcgggta ttattaaaaa
    21421 ccagaagatc gccggggttg agcttatcaa gtaaatcggt gaaagtaccg tgcgtcagcg
    21481 cgcccgtcgg cccgtccagc gacagtaaac gacagctact gcgttcaggc atgggatagt
    21541 gggcaatcag ggattcgggc aattcaaagg agaaatcggt aacgcgcatg acactgactc
    21601 agactaaaat aagaggcggg tagtctagtg ccggggcgct ctccctgcaa cctttacccc
    21661 cttttggata gaatataatc gatgaatttt ttagctcacc tgcatttagc ccatctcgcg
    21721 gaaagctcgc tttccggcaa tttactggct gatttcgtac gcggaaatcc tgaagaaagt
    21781 tttccgcccg acgtcgtggc tggcattcat atgcatcgac gtatcgacgt attaaccgac
    21841 aatctgccgg aagtccgcga agcacgggaa tggtttcgta gtgaaactcg ccgcgttgcg
    21901 cctattacgc tggatgtcat gtgggatcac tttctttccc gccactggtc gcagctgtcg
    21961 ccggactttc cgctacagga atttgtctgt tatgcccgcg agcaagtgat gacgattttg
    22021 ccggactcac cgccacgttt tatcaatctg aacaattact tgtggtcaga gcagtggctg
    22081 gtgcgctatc gcgatatgga tttcatccag aacgtgttaa acggcatggc aagccgccgc
    22141 ccacgtctgg atgccctgcg tgactcctgg tacgatttag acgctcatta tgacgccctc
    22201 gaaacccgct tctggcagtt ttatccgcgg atgatggcgc aggcgtcaca caaggcgtta
    22261 taaaacgtta gttcatccat accgtagccg aaatggcagg caaagccaga atcccgtcag
    22321 tcagttgccc atgcctttct ttgcattgcc attgcacggc attgagcaac ggtgacgcgg
    22381 gtagcacgac ttcacaagct tcgccacggt tgattgccac cagtacacgc tgctgattca
    22441 gcacgcggac aaataccacc acgttatctt ccgcatacag cacctgacag ccgccatgac
    22501 gtagcgcctg acttttctta cgcagcgcaa tcattcgctg gtacagcgcg aataacgccg
    22561 tatcctgctt ttccacctgc caggggaacg gtttacggca aaacggatcg tttttgccat
    22621 ccagtcctac ttcatcaccg taataaatgc acggtacacc aggccaggtg aacagccaga
    22681 ccaccgccag cggcaggcgc gcaatatccc gaccgagcag cgttttaaat cgcgcagtat
    22741 cgtggctgtc gagctgatta aacatacgta attgttgttg atgagaaagc cctgcgcggt
    22801 aattatccat ccaggccata caggtttggg catcaatttg ctgcggatcg taagagatat
    22861 cggtattggc aagaaatccc cacaacggga atgtgaagcc acgatagttc atggcggcat
    22921 cttccacatc ggcctgtaac cattgccgtg catcgccaaa atgttcgccg acaatatacg
    22981 cttccggctg ggtttctttc gccgcttcgg tgctcccagc aacgtgctgc atattattgc
    23041 gcgccccacc cgcctcccct agcatatgca ccacatccag ccgccagccg tccatactcc
    23101 acggcgcttt cagccaatgg cggacaatac tgtcttcccc gcgataaatt tcattcacca
    23161 gactttccga ctgataatcc agcttcggca agctggcata gccaagccag tcgagcgccg
    23221 tgccatcatc actaaacgag taccagtcgc gccagggcga ttcagggttg tgacaagctc
    23281 cccccgtgcc acgattgtgc ctgtcaaacc aggcatggga atcgccactg tggttaaaca
    23341 cgccatccag ccccagccgc attcccagct gctgcgtatt gtggcgtaag cgcagcaacg
    23401 ccccatcacc gccaaactgc ggatcgacat ggcgataatc ctcggtatcg tatttatgta
    23461 cgctgggagc tttaaacacc ggattgagat acagcgccgt cacgccaagc tttttcagat
    23521 acggcagttt ttcgcttatc ccgtccagat cgccgccata gaacgttgat ccgcccgcct
    23581 gcgccgtgac cggttcatcc cagtcacgca agatgatctc ttgtccggct gcatgatggt
    23641 aatagacatg atcctgttca gcttcacgag gaagactacg cgcaaaacga tcagggaaga
    23701 tctgataaaa aatctgatcc gcagcccatt gtgggccgat atccggtaca tcgacggcaa
    23761 actgctccag tcgtgccggc ggcattcggc tgaagccctg cggtgtaaac caacgctggc
    23821 gatcgtgcca cagcagtttg aaactgtaac gccgccgggg ttgtccgctg gagagatcaa
    23881 tcgccgcacg ccatgcggtg acgccaggct gctgctgact gcgatgctta tgcatcgata
    23941 ctgacatttc ttcgttatcg tgttctgtac gcagcataat gcgctgcggt gggtcttcgc
    24001 ccgtcagcca cagtgtaatg agcagttgat ctttgctttg tttaacaaat gggggcaccg
    24061 gcaggtgcca tgcatttaac atcataattc ccctgtgatg aaaatctgat caccttgcca
    24121 tagcctaatg caggatcact catcatctga aggatatttg ggggaggagg acgggggcgg
    24181 ataactttcg gaatagccgg atcaggattt cctcctgaac cggcttacag aacgcaagga
    24241 ttactggttt tcagccagct gacgatcgtg gcgacgctta aacatccagc caatcaacag
    24301 cacaacaatc cacgcgaaac cgacatacag cgagatacgc gtatccgggt gataaccaat
    24361 caacccgata ataaagagca ggaaaatcag cccgccgatg gtcgttgcta ccccacccgg
    24421 cactttaaat ttcagcgcct taacttcttc tggcggcaaa cagcgacgga aggcaatttg
    24481 cgacagcagg atcataatcc acacccacac cgtggcgaag gttgccagcg aagcgatcac
    24541 caggaagacg ttttccggca tgatgtagtt cagatacacc gcaaacagca gcgcggtagt
    24601 catcaccagc accgtaaccc acggaatacc gcgacgcgac gttttgctga aaattttcgg
    24661 cgcgctgccc tgctctgcca taccgtggag catacggcct acgccaaaga cgtcactgtt
    24721 aattgccgat agcgaagcgg tcagcacaac aaagttaaga atgctggcgg caaaggtaat
    24781 gcccatatgc tggaacgtca gcacgaacgg gctaccggca gtgccaacct gattccacgg
    24841 gtagatagac ataatgacga acagcgtacc gacgtagaac accagaatac gcatcggcac
    24901 ggagttaatc gcacgcggta tcgatttctc aggatctttc gcttcaccgg cggtaatccc
    24961 gataatttcg atcccaccgt aagcaaacat caccatttgc aacgacatca ccatcccaag
    25021 ccagccgtta ctgaagaagc cgccgttgct ccacagatta tgaataccgg tcggttgccc
    25081 gccgttgcca atcccccaga tgatgatgcc gaaaccagcg acaatcatga tgatgatggt
    25141 ggcgacttta aagaacgaga accagaattc cagctccccg aagaccttca cgctcatcag
    25201 gtttacggcg caaatgatca gcaccacgct cagtacccaa atccagtgcg gcaccgtcgg
    25261 gaaccagaca cccatataga taccaaaagc ggtcacatcg gcgatggcga caataaggat
    25321 ttcaaagcag taggtccagc cggtaatgta acctgccagc gggccgaggt tttcctgcgc
    25381 ataacgcgag aaagagctgg cggccgggtt atgtaccgac atttccccca gcgcacgcat
    25441 aatgatatac gccgcgatac caccgataat ataggccaac aacacgctcg gaccggccat
    25501 tttgatggcg tctgccgaac cgtaaaacag cccggtacca attgctgaac ccagtgccat
    25561 aaagcgtatg tggcgggtac ttagcccacg ctttagcttg ttcttacttt ccatcaattc
    25621 ctaacccatc aacacaataa aaaaccacgg ggcatcgcag ccccgtggtt aaaacaaatg
    25681 ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct gccgcgcgat cccagataat
    25741 ggccagaacc accatcacca ctgttggcat taaccacgcc agaccttgtt cggccagcgg
    25801 taaacgctgc gcccaggacg gtaagatatc gctgaatgca gatgctttga tcccgtcgag
    25861 aataccaaaa agcaggctga taaacatcgg cggagcaatc acgcgggacg aattatgcca
    25921 ccatgagcgt gtaaaactta atacaaccag tgcgatacac ggcggataaa tagcggtcag
    25981 caccggtacg gagatctgga tcagctggct taagccgagg ttagaaacca ccatcgagaa
    26041 gccgccgagg ataaacacca gcgtacgata agagagcggt acgtactggg caaagaattc
    26101 tgcacaagca caggtcaggc caactgccgt taccaggcag gcgatgaaga ttaacgccgc
    26161 cagcaggaag ctaccgccgc cgccaaaggt gtgctgaacg taagcatgca gaatagcagc
    26221 gccgtttgca gactgatcga ccagcgacgc gctgtctgaa cccagacgga acagcgccag
    26281 gtacagcaga gtcagaccaa cacccgccat caggccagcc cagacggtat aacgggtcag
    26341 cagacgcgct tcggtaacgc cacgagaacg cgccgcgtta acaataacga taccaaacac
    26401 cattgcgccc agcgtatcca tggtcagata gccgttaacg aagccgttag aaaacgcagc
    26461 gttttgataa gcctcagtcg ccgtgctgat agaacccgcc ggccagacaa tcgcggcaac
    26521 agacaggatg accagcgcga taattttcag cggcgcaagg aagttgccca cggtatccag
    26581 cagcttgccc ggatagagcg aaaccagaat aacgatagcg aaatagacca ggctgtagat
    26641 aaacagcggc agcgcggaat caccagtcag cggcgcaatc cccacttcaa aggaaacggt
    26701 agctgtacgc ggcgtagcga aaagcggccc caccgccagg taacaaaccg ttgccagcag
    26761 cacgccagcg actttaccga ttggcgtgct gaggctgtca acaccgccgc caacttttgc
    26821 cagcgccact accgttaata ccggcaggcc aacggcagta atgaggaagc cgaatgccgc
    26881 agtccagacg tgttcgccag cctgcaagcc gaccattgga gggaaaataa tgttacctgc
    26941 gccgacgaac aacgcaaatg tcataaagcc cagagcgatg atatcgcgcg atcttaattg
    27001 atgggtcata aatcatactg cctgtggatg tggtgttgta acgttaaatt tttgtgccaa
    27061 tccctgggga tgatacagcg gtatatttaa acaattttag cgggaaatac tcccaaccag
    27121 gcgcggcgaa gaatagtttt tcgatttacc gcgttaatgc agtcagtatg acagcacctg
    27181 aagcgcaatt taaacgctta taacgtttta aagcaaggag ggatctaaaa accagaggta
    27241 tatagcgata agaaatcacc aaccagtata aaacactatt tccgtgaaaa agacatggct
    27301 aatcatgcga acaaaaaatc accaaccgtt gtaaaatcta acgcccgtat tttgactgca
    27361 aagaaaataa gccagcatcg actggcttat ggcaataaaa gatgacaaag gcggattaat
    27421 cgctgttttt ggcaattaaa cgttccggga taacaaaact gaaacgtgtt ccttttccta
    27481 ctgtactctc aatattcagg cgactttcgt gatgattcac agcatgtttc acgatcgcta
    27541 accctaatcc gctaccgccg gtttgccggg aacgcgcttt atcaacgcga taaaaacgct
    27601 cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc gttatcttca acgctaaatt
    27661 cggcaccgtg cggcactcgc tgccagcgta cggtgatatg cgtgccttct ggagtatgat
    27721 tcacggcgtt atagaccagg ttcgaaatcg cactgcgtag ctgatcttcg ttgccagaca
    27781 ctttgaggcc gttatctatc tcaaaggtaa atgtctgttt tttctgactc agagtctgag
    27841 cctcgcgctc aacaacgcgc agcatcatcg gcacatcaac cttttcattg agcaattgcg
    27901 tcggcgcggc ttcaattttc gacagcgtta gcaactgctt caccagccct tccatccgct
    27961 gggtctgctc gcgcatggtg tgcaacgctt tttcgcgtac cgcgccttcc agcggctgct
    28021 catccatcat ctccaggtaa ccctgtaaca cggtcaatgg cgtacgtaac tcatggctca
    28081 cgttggcaaa aaagttacgc cgcgcccctt ccagttgatg catttgcgtg acatcacgcg
    28141 ccaccatcag caactgtttg tgggtataag gcatgacgcg aatttccaga tgccgcccgg
    28201 tgttgagcac cagattgagc gggcgagaaa aatcacgcgt tttcagatat tgcgtaaact
    28261 ccgggtaacg cagtaggtta aggatgttct gcccgttatc ttccggccag cgcaaaccaa
    28321 gaatttgttg cgccagaccg ttacaccaga aaataccgct ctcttccgtg gtcagcacca
    28381 ccgcgtcggg cagcgactcc gcgccgctac gaaagcgttt aatcagattg cccagttcac
    28441 ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc gtatagtagc ggttcccagc
    28501 taccacgccc cggtggcggg gtcatactgc gatccaccca cagccaccat gaaaggcgca
    28561 ataaattcca gaaatgccag ataagcagtc ctgttaccga tgccagcaaa aaccagggca
    28621 ggtaaccaaa aaatgcaccc aggatgaaag ccgggaggca gcaaagtagc agctccagca
    28681 ccagcctttt ccacgacagc cgttccagca cgtaagatac tccagttaag aaatcataag
    28741 ccctgctctg cgtccgatga gcaaggcgtt aaaagcgggt tgaaaaacga tatcctgtac
    28801 cgcgcacggt ctgcaccatg cggtcatgcc cgccgggctc cagtgcttta cgcaggcgac
    28861 gaatgtggac atcgaccgtg cggtcttcca catacacgtt agttccccag acgtggttta
    28921 acagctgctc gcggctgtac acgcgctcag gatgcgtcat aaaaaagtgc agcagtttaa
    28981 attctgtcgg ccccatctcc agcggctctt cgcccgccat cactcggtga gatgtcgggt
    29041 cgagacttaa tccctgcatc tcaatcacct cttccaccgc cattggcgaa atacggcgca
    29101 ttaccgcttt gattcgcgcc accagctcct tcggcgaaaa cggcttggtg atatagtcat
    29161 ccgcgccggt ttcaaggccg cgcacgcgat cttcttcttc ccctctggcg gtcaacatca
    29221 ccactggaat atcccgggtc atcgactcgc gcttaaggtg tttgatgaac tggataccgg
    29281 agccgccagg taacatccag tcaaggagaa ttaaatccgg ccagggttca ttcagttgat
    29341 tcacagcact gtcataatct tccgcttcga ccggctgaaa gccattttgt tcgagcacga
    29401 agcagaccat ttcgcgaatt ggagcttcat cttctacgac cagaatacgt ctcgccatga
    29461 tttgccctgt tgtaataaat aggttgcgat cattaatgcg acgtcattat gcgtcagatt
    29521 tatgacagat ttatgaaaag ctcgtcgcac atatcttcag gttattgatt tccgtggcgc
    29581 agaaaaaagc aaatggcaca tctgtttggg tataatcgcg cccatgcttt ttcgccaggg
    29641 aaccgttatg cgcatccttc acacctcaga ctggcatctc ggccagaact tctacagtaa
    29701 aagccgcgaa gctgaacatc aggcttttct tgactggctg ctggagacag cacaaaccca
    29761 tcaggtggat gcgattattg ttgccggtga tgttttcgat accggctcgc cgcccagtta
    29821 cgcccgcacg ttatacaacc gttttgttgt caatttacag caaactggct gtcatctggt
    29881 ggtactggca ggaaaccatg actcggtcgc cacgctgaat gaatcgcgcg atatcatggc
    29941 gttcctcaat actaccgtgg tcgccagcgc cggacatgcg ccgcaaatct tgcctcgtcg
    30001 cgacgggacg ccaggcgcag tgctgtgccc cattccgttt ttacgtccgc gtgacattat
    30061 taccagccag gcggggctta acggtattga aaaacagcag catttactgg cagcgattac
    30121 cgattattac caacaacact atgccgatgc ctgcaaactg cgcggcgatc agcctctgcc
    30181 catcatcgcc acgggacatt taacgaccgt gggggccagt aaaagtgacg ccgtgcgtga
    30241 catttatatt ggcacgctgg acgcgtttcc ggcacaaaac tttccaccag ccgactacat
    30301 cgcgctcggg catattcacc gcgcacagat tattggcggc atggaacatg ttcgctattg
    30361 cggctccccc attccactga gttttgatga atgcggtaag agtaaatatg tccatctggt
    30421 gacattttca aacggcaaat tagagagcgt ggaaaacctg aacgtaccgg taacgcaacc
    30481 catggcagtg ctgaaaggcg atctggcgtc gattaccgca cagctggaac agtggcgcga
    30541 tgtatcgcag gagccacctg tctggctgga tatcgaaatc actactgatg agtatctgca
    30601 tgatattcag cgcaaaatcc aggcattaac cgaatcattg cctgtcgaag tattgctggt
    30661 acgtcggagt cgtgaacagc gcgagcgtgt gttagccagc caacagcgtg aaaccctcag
    30721 cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca ctggaagaac tggatgaatc
    30781 gcagcagcaa cgtctgcagc atcttttcac cacgacgttg cataccctcg ccggagaaca
    30841 cgaagcatga aaattctcag cctgcgcctg aaaaacctga actcattaaa aggcgaatgg
    30901 aagattgatt tcacccgcga gccgttcgcc agcaacgggc tgtttgctat taccggccca
    30961 acaggtgcgg ggaaaaccac cctgctggac gccatttgtc tggcgctgta tcacgaaact
    31021 ccgcgtctct ctaacgtttc acaatcgcaa aatgatctca tgacccgcga taccgccgaa
    31081 tgtctggcgg aggtggagtt tgaagtgaaa ggtgaagcgt accgtgcatt ctggagccag
    31141 aatcgggcgc gtaaccaacc cgacggtaat ttgcaggtgc cacgcgtaga gctggcgcgc
    31201 tgcgccgacg gcaaaattct cgccgacaaa gtgaaagata agctggaact gacagcgacg
    31261 ttaaccgggc tggattacgg gcgcttcact cgttcgatgc tgctttcgca ggggcaattt
    31321 gctgccttcc tgaatgccaa acccaaagaa cgcgcggaat tgctcgagga gttaaccggc
    31381 actgaaatct acgggcaaat ctcggcgatg gtttttgagc agcacaaatc ggcccgcaca
    31441 gagctggaga agctgcaagc gcaggccagc ggcgtcacgt tgctcacgcc ggaacaagtg
    31501 caatcgctga cagcgagttt gcaggtactt actgacgaag aaaaacagtt aattaccgcg
    31561 cagcagcaag aacaacaatc gctaaactgg ttaacgcgtc aggacgaatt gcagcaagaa
    31621 gccagccgcc gtcagcaggc cttgcaacag gcgttagccg aagaagaaaa agcgcaacct
    31681 caactggcgg cgcttagtct ggcacaaccg gcacgaaatc ttcgtccaca ctgggaacgc
    31741 atcgcagaac acagcgcggc gctggcgcat attcgccagc agattgaaga agtaaatact
    31801 cgcttacaga gcacaatggc gcttcgcgcg agcattcgcc accacgcggc gaagcagtca
    31861 gcagaattac agcagcagca acaaagcctg aatacctggt tacaggaaca cgaccgcttc
    31921 cgtcagtgga acaacgaact ggcgggttgg cgtgcgcagt tctcccaaca aaccagcgat
    31981 cgcgagcatc tgcggcaatg gcagcaacag ttaacccatg ctgagcaaaa acttaatgcg
    32041 cttgcggcga tcacgttgac gttaaccgcc gatgaagttg ctagcgccct ggcgcaacat
    32101 tccgagcaac gcccactgcg tcagcgcctg gtcgcgctgc atgggcagat tgttccccaa
    32161 caaaaacgtc tggcgcagtt aatggtcact atccagaatg tcactctgga acagacgcaa
    32221 cgtaatgtcg cacttaacga aatgcgccag cgttataaag aaaagacgca gcaacttgcc
    32281 gatgtgaaaa ccatttgcga gcaggaagcg cgcatcaaaa cgctggaagc tcaacgtgca
    32341 cagttacagg cgggtcaacc ttgtccactt tgtggttcca ccagccaccc ggcggtcgag
    32401 gcgtatcagg cgctggagcc tggtgttaat cagtctcgat tactggcgct ggaaaacgaa
    32461 gttaaaaagc tcggcgaaga aggtgcggcg ctgcgcgggc aactggatgc attaacgaag
    32521 cagcttcagc gcgatgaaaa cgaagcgcaa agcctccgac aagatgagca agcacttact
    32581 caacaatggc aagccgtcac gaccagcctc aatatcacct tgcagccaca ggacgatatt
    32641 caaccgtggc tggatgcaca agatgagcac gaacgccagc tgcggttact cagccaacga
    32701 catgaattac aagggcagat tgccgcgcat aatcagcaaa ttatccagta tcaacagcaa
    32761 attgaacaac gccagcagca gctcttaacg gcattgacgg gttatgcact gacattgcca
    32821 caggaagatg aagaagagag ctggttggcg acacgtcagc aagaagcgca gagctggcag
    32881 caacgccaga acgaattaac cgcgctgcaa aaccgtattc agcagctgac gccgattctg
    32941 gaaacgttgc cgcaaagtga tgatctcccg cacagcgaag aaactgtggc gctggataac
    33001 tggcggcagg ttcatgaaca atgtctcgca ttacacagcc agcagcagac gttacagcaa
    33061 caggatgttc tggcggcgca aagtctgcaa aaagcccagg cgcagtttga caccgcgcta
    33121 caggccagcg tctttgacga tcagcaggcg ttccttgcgg cgctaatgga tgaacaaaca
    33181 ctaacgcagc tggaacagct caagcagaat ctggaaaacc agcgccgtca ggcgcaaacg
    33241 ctggtcacac agacagcaga aacgctggca cagcatcaac aacaccgacc tgacgggttg
    33301 gctctcactg tgacggtgaa gcagattcag caagagttag cgcaaactca ccaaaagttg
    33361 cgtgaaaaca ccacgagtca aggcgagatt cgccagcagc tgaagcagga tgcagataac
    33421 cgtcagcaac aacaaacctt actgcagcaa attgctcaaa tgacgcagca ggttgaggac
    33481 tggggatatc tgaattcgct aataggttcc aaagagggcg ataaattccg caagtttgcc
    33541 caggggctga cgctggataa tttagtccat ctcgctaatc agcaacttac ccggctgcac
    33601 gggcgctatc tgttacagcg caaagccagc gaggcgctgg aagtcgaggt tgttgatacc
    33661 tggcaggcag atgcagtacg cgatacccgt accctttccg gcggcgaaag tttcctcgtt
    33721 agtctggcgc tggcgctggc gctttcggat ctggtcagcc ataaaacacg tattgactcg
    33781 ctgttccttg atgaaggttt tggcacgctg gatagcgaaa cgctggatac cgcccttgat
    33841 gcgctggatg ccctgaacgc cagtggcaaa accatcggtg tgattagcca cgttgaagcg
    33901 atgaaagagc gtattcctgt gcagatcaaa gtgaagaaga tcaacggcct gggctacagc
    33961 aaactggaaa gtacgtttgc agtgaaataa ctattcagca ggataatgaa tacagagggg
    34021 cgaattatct cttggccttg ctggtcgtta tcctgcaagc tatcacttta ttggctacgg
    34081 tgattggtag ccgttctggt ggttgtgatg gtggtatgaa aaaagtcatt ttatctttgg
    34141 ctctgggcac gtttggtttg gggatggccg aatttggcat tatgggcgtg ctcacggagc
    34201 tggcgcataa cgtaggaatt tcgattcctg ctgccgggca tatgatctcg tattatgcac
    34261 tgggggtggt ggtcggtgcg ccaatcatcg cactcttttc cagccgctac tcactcaaac
    34321 atatcttgtt gtttctggtg gcgttgtgcg tcattggcaa cgccatgttc acgctctctt
    34381 cgtcttacct gatgcttgcc attggtcgcc tggtttccgg ctttccgcat ggcgcatttt
    34441 ttggcgtcgg cgcgatcgtg ttatcaaaaa ttatcaaacc cggaaaagtc accgccgccg
    34501 tggcggggat ggtttccggg atgacagtcg ccaatttgct gggcattccg ctgggaacgt
    34561 atttaagtca ggaatttagc tggcgttaca cctttttatt gatcgctgtt tttaatattg
    34621 cggtgatggc atcggtctat ttttgggtgc cagatattcg cgacgaggcg aaaggaaatc
    34681 tgcgcgaaca atttcacttt ttgcgcagcc cggccccgtg gttaattttc gccgccacga
    34741 tgtttggcaa cgcaggtgtc tttgcctggt tcagctacgt aaagccatac atgatgttta
    34801 tttccgtttt ttcggaaacg gcgatgacct ttattatgat gttagttggg ctagggatgg
    34861 tgctgggaaa tatgctaagt ggcaggattt caggacgtta ttcaccactg cgcattgcag
    34921 cagtgactga ctttatcatt gtactggcac tgctgatgct ctttttctgc ggcggcatga
    34981 aaacaacgtc gcttattttt gcttttattt gttgcgcggg attatttgcc ctttcagcac
    35041 ctctgcaaat attgttactg caaaacgcca aaggcggaga gttattaggt gccgcaggtg
    35101 ggcaaatagc gtttaacctc ggtagcgccg tcggcgcata ttgcggaggt atgatgctga
    35161 cgctggggct ggcatataat tacgtggcgc tgcctgccgc cctgctttcg tttgctgcga
    35221 tgtcatcgtt gctgctgtat ggtcgctata agcgccagca agcggcggat agtccggtgc
    35281 tggcgaaacc actggggtag attactgtca cggtgcgcgt tgcttattgc cggatgcggc
    35341 gtgaacgcct tatccgccct acgcggttct ggcacatttt gtaggcctga taagacgcgg
    35401 caagcgtcgc atcaggcatg atgcgccaat tgcctacgtt ttttactctt gtggccataa
    35461 ccacgcagcg ccgcgtacgc cgctggaatc accgtgcttc gccttacgca ccggcgtttc
    35521 acattcgccg ccgaagacaa attgtttaat caactgccca accgtttgat ataaacggtc
    35581 tacattgctc atcccgcccc ccaggacaat cacatccgga tcgagaatat tcacgacatg
    35641 tgccagcgat tttgccagcc gcagctcgta gcgacgcaat gccagttccg ctaccggatc
    35701 gctttcttca accaggcgga taatttcact gcctttcagc gcatgtccgc tcaaacgacg
    35761 ataatccgtc gcgaatcccg tgcccgaaat aaaggtttca atacaacctt gtttaccgca
    35821 ataacaaggg acttcctcgc gataacgcag ctcgtcttca tccatccacg gtagcggatt
    35881 gtgtccccac tcgcctgccg tgccattgcc gccgatatgc gcccgcccat ttaatgccac
    35941 gcccgcgccg catcccgtgc cgataatcac ggcaaatacc gtctgcgctc ccgctgccgc
    36001 gccatctact gcttctgaaa ccgccagaca gttggcatca tttgccagcc gcacttcccg
    36061 ctgcaacctc gcgcttaagt ctttatcgaa tggctgaccg ttgagccagg ttgaattggc
    36121 attcttcacc acaccggtgt agggcgaaat cgaacctgga atgcccatac ctaccgttcc
    36181 gcgctgccct gtcgcctgct ccgccatatc aaccaacgtg gcgatcgttt caatagtctg
    36241 ccggtaatca tcacgcggcg tgggcagacg atggcggtac aactgctccc ctgcatcgcc
    36301 cagtgcaatc acttcagttt tggtgccgcc taaatcgata cctatacgca ctgtactctc
    36361 cttatttttt tcaatatcaa tagcgtagag acggacaacc ggattggcaa tgcaaggccg
    36421 ccgacaattc gttatcatgc ccgctaaatt taacgacaag gccgtggaaa ttatcatgct
    36481 gtggttcaaa aatttaatgg tttaccgtct tagccgcgag atttcgctgc gtgcagaaga
    36541 gatggaaaaa cagctagcct cgatggcatt taccccatgc ggcagccagg acatggcgaa
    36601 gatgggctgg gttcctccga tgggatcgca cagcgatgcg ttaacgcacg ttgccaatgg
    36661 tcaaattgtt atctgcgcgc gcaaagaaga aaaaatcctc ccgtctccgg tgattaaaca
    36721 ggcgctggaa gcgaaaatcg ccaaactgga agcggaacag gcgcgtaagc tgaagaaaac
    36781 cgaaaaagat tcgctgaaag acgaagtgct gcactctctg ctgccgcgtg ctttcagccg
    36841 ttttagccag acaatgatgt ggatcgacac ggttaacggt ttgattatgg tggactgcgc
    36901 cagtgccaaa aaagcggaag atacgctggc attactgcgt aaaagcctgg ggtcgttacc
    36961 ggttgtacca ttgagcatgg aaaacccgat tgaactgacg ctgaccgaat gggttcgctc
    37021 cggtagtgcg gcacagggct tccagctgct tgatgaagcc gagctgaaat cgttgctgga
    37081 agatggcggc gtgatccgcg cgaagaaaca agatctgacc agcgaagaga tcaccaatca
    37141 cattgaagcc ggaaaagtgg tgactaaact ggcgctcgac tggcagcagc gcattcagtt
    37201 tgtgatgtgc gacgatggtt cgctcaagcg tcttaagttc tgcgacgagc tgcgcgatca
    37261 aaacgaagat atcgaccgtg aagatttcgc cctgcgtttt gatgccgatt tcatcctgat
    37321 gactggtgaa ctggcagcgt taattcaaaa cctgattgaa ggattaggtg gcgaagcaca
    37381 acgttaattg ctgattttcc tttaatgccg gatgcgacgc ctgccgcgtc ttatccggcg
    37441 tacgaagcca caccaggcat ataattattc gctacggcga gcaataattt ttagcgcagc
    37501 aatattatgc gttttacgcc gtaactgatc taatcgcctc atccctattc gcctgtcagc
    37561 agcacgcaga atattcacta acccccattt atcgctggta aattgttttg ttaacagctc
    37621 cgcttttggg gtatcaatat attcacgcaa taaatcagaa attgaacaaa tatccgtcag
    37681 gtaaggatac cagcaagttt caccgtgaaa attacgcact ccagcgttac ctgcaataaa
    37741 gtctttcggg taatcccaga tcacgttttt atccaacgtg atccaataac gcggtaaatc
    37801 ggtacttcca ccattttggc tgcgcatgag ataacgggta cagtgaattt gcaggttgat
    37861 cgttggcgtc agtaagtcat agatctcccg ctgtaacttg ctccatggac gttgtgtcat
    37921 tgtttttcct caagccggtg acgcgcttca ctgcgaattt tccgcgttgc cccggttttc
    37981 actaaatcac gtaactgcga aagatcgggt tgttcgacaa ataagagtaa tgatagatcc
    38041 tcttttaacc acgccgtttt cacatcggca gccagctgtg gattattaat agccagcgac
    38101 cgatcctgag actcagtcaa tgtcgttaac aacgacgccg gggtatgtgc gttgcgtaat
    38161 gccgtttggc gcaaatgatc attgcgactt tgcagcaatg cacgccacca ttcatcatct
    38221 gatttatctt ttatcgtagc cagttccgca aaaaatacct ggagatcttc actgatattt
    38281 atatcaacct tgatgaatta tgaatatatc ggaaattgaa aaaatcgcag caaagga
//
LOCUS       DAKISA010000035        37276 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.035, whole genome shotgun sequence.
ACCESSION   DAKISA010000035 DAKISA010000000
VERSION     DAKISA010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37276
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.035"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..642)
                     /locus_tag="OVW45_001814"
     CDS             complement(<1..642)
                     /locus_tag="OVW45_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HCV5526281.1"
                     /translation="MVKKPQQGTIFAAAQRSDSYFVKCINMLSLYEKIKIRLIILFLL
                     AALSFIGLFFIINYQLVSERAVKRADSRFELIQKNVGYFFKDIERSALTLKDSLYLLK
                     NTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPLVDES
                     GRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDL"
     gene            complement(742..1653)
                     /gene="gsiD"
                     /locus_tag="OVW45_001815"
     CDS             complement(742..1653)
                     /gene="gsiD"
                     /locus_tag="OVW45_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCV5526282.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1656..2576)
                     /gene="gsiC"
                     /locus_tag="OVW45_001816"
     CDS             complement(1656..2576)
                     /gene="gsiC"
                     /locus_tag="OVW45_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCV5526283.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGLEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2594..4132)
                     /gene="gsiB"
                     /locus_tag="OVW45_001817"
     CDS             complement(2594..4132)
                     /gene="gsiB"
                     /locus_tag="OVW45_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCV5526284.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKTFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4152..6023)
                     /gene="gsiA"
                     /locus_tag="OVW45_001818"
     CDS             complement(4152..6023)
                     /gene="gsiA"
                     /locus_tag="OVW45_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCV5526285.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6010..6975)
                     /gene="iaaA"
                     /locus_tag="OVW45_001819"
     CDS             complement(6010..6975)
                     /gene="iaaA"
                     /locus_tag="OVW45_001819"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCV5526286.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCIGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7179..8414
                     /gene="moeA"
                     /locus_tag="OVW45_001820"
     CDS             7179..8414
                     /gene="moeA"
                     /locus_tag="OVW45_001820"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCV5526287.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8414..9163
                     /gene="moeB"
                     /locus_tag="OVW45_001821"
     CDS             8414..9163
                     /gene="moeB"
                     /locus_tag="OVW45_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCV5526288.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASLYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     TSGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9342..10004)
                     /gene="fsa"
                     /locus_tag="OVW45_001822"
     CDS             complement(9342..10004)
                     /gene="fsa"
                     /locus_tag="OVW45_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCV5526289.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            10135..11034
                     /locus_tag="OVW45_001823"
     CDS             10135..11034
                     /locus_tag="OVW45_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCV5526290.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            11040..13472
                     /locus_tag="OVW45_001824"
     CDS             11040..13472
                     /locus_tag="OVW45_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCV5526291.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13618..14433
                     /gene="ybiV"
                     /locus_tag="OVW45_001825"
     CDS             13618..14433
                     /gene="ybiV"
                     /locus_tag="OVW45_001825"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCV5526292.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14585..15850
                     /locus_tag="OVW45_001826"
     CDS             14585..15850
                     /locus_tag="OVW45_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCV5526293.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSNMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGIDS"
     gene            complement(15817..16977)
                     /locus_tag="OVW45_001827"
     CDS             complement(15817..16977)
                     /locus_tag="OVW45_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCV5526294.1"
                     /translation="MGFFAHENSVISELEADHKMDKKLKIADIAARTGLSPSTVSRVL
                     AGKANTSARAREQVLACARELGVMDGMAAGRMLLNNLIIFAPQRAFDQRSDIFYYRVI
                     QSISKALSPHEVRLRYCALEEFDSDPALFLSRMNEADTQAAILLGIDDPHIHDLAADL
                     AKPCVLINCRDERMRLPAIAPDHRLIGAFAARYLFEMGHREVMNVMCLRRYTMEQRFA
                     GIKESWRQHNLAFHHDRHLLNISSFSAKEAEEKVGAWLDAANGKTLPTAFLVGGDFMA
                     AGTVNALHRRGLRVPQDVSVMSIDGFNLAAIEDVPLTAVHVPRDELGNEAVQMLQQRL
                     IRPDAPVGSLLLHGRLAVRESVRRVRPGKGNTAVEREGLYDTQLSIPNARLP"
     gene            17215..18516
                     /locus_tag="OVW45_001828"
     CDS             17215..18516
                     /locus_tag="OVW45_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCV5526295.1"
                     /translation="MSQGINNDMTASKPRHIVKNLRWWILVLFLLGVTVNYITRNSLG
                     ILAPELKSSLGITTEQYSWIVGAFQLAYTLFQPLCGWLIDVIGLKLGFMICAGLWALA
                     CLMHAGAGNWVHLALLRFFMGGAEAAATPANAKTIGEWFPKSERPIAAGWAGVGFSIG
                     AMLAPPIIYFAHASFSWQGAFMFTGILAMLWVVLWWAFYHSPDKHPNLSKAELDFIRQ
                     DNEAPPVKLPFLTALKTVGKNKRFYGIAIPAFMAEPAWAVMSFWVPLYLSKTYGMDLK
                     QIALFAWLPFLAADLGSIASGYLTKLYTRLFGLSRVNSVVASSVSGAFLMVSLAVMAF
                     TNNPYIAIVLISIGGFGHQIISCMLSALVVESFDKGQMATVNGMRGSAAWIASFLFSL
                     IIGVTADKIGFNPLFIAMGFFDLIGAFFLVTFIAERRAKRA"
     gene            18531..20894
                     /locus_tag="OVW45_001829"
     CDS             18531..20894
                     /locus_tag="OVW45_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HCV5526296.1"
                     /translation="MKTLKNWTLHKQSAHHVELLVDGQHILCLYVLEENLFRVLLKRK
                     GELALDRTWSIAPAQDVPWEGRSREDLSGFSLPAWTLIQHDDTVVIATESLRATVHQP
                     LWLEWHYRNNEGEWLPLVNDRPTSAYLLNAHGDGVAHYQSRRKDERFYGLGDKAGDLQ
                     RNGKRYEMRNLDAMGYNAVSTDPLYKHIPFTITRRDDISFGLFYDNLSSCWLDLGNEI
                     DNYHTAYRRWQAEAGDIDYYLFTGERVLDITKAFVRLTGKTLFGPKWSLGYSGSTMHY
                     TDAPDAQNQLMNFIRLCEEHAIPCDSFQLSSGYTSINGKRYVFNWNYDKVPQPKVMSQ
                     AFHNAGLHLAANIKPCLLQDHPRYNEVAERGLFIRDSETDEPERSSFWDDEGSNLDFT
                     NPQTIQWWQEGVTTQLLEMGIDSTWNDNNEFEVWDGEARCHGFGQTIAIKHIRPVMPL
                     LMMRASMEAQQRFAPEKRPYLISRSGCAGMQRYVQTWSGDNRTSWDTLRYNTRMGLGM
                     SLSGLYNLGHDVGGFSGDKPDPELFVRWVQNGVMHPRFTIHSWNDDHTVNEPWMYPGV
                     TPAIRSAIELRYRLLPYFYTLMWQAYADDEPMLRPTFLDHEHDVQTFEECDDFLLGRD
                     ILVASVVEQGERQRRVWLPDNETGWYDFYNGEWFSGGQWITLDAPLEKLPLMVRAGAG
                     LPLSKRITYVSAEQDDTRELKLFPLKGVGTTSGLLFEDDGESWGYQTGNALWVEWEMV
                     CDGATINLKVNARGDYRPAWSALKVSLPLEEKRTLLVNGVEGSEWMR"
     gene            complement(21074..22669)
                     /locus_tag="OVW45_001830"
     CDS             complement(21074..22669)
                     /locus_tag="OVW45_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCV5526297.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERAIDFSGNYEDYLRSKGIDA"
     gene            22888..23808
                     /gene="ldtB"
                     /locus_tag="OVW45_001831"
     CDS             22888..23808
                     /gene="ldtB"
                     /locus_tag="OVW45_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCV5526298.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(23867..24985)
                     /locus_tag="OVW45_001832"
     CDS             complement(23867..24985)
                     /locus_tag="OVW45_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCV5526299.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(24982..25449)
                     /gene="mntR"
                     /locus_tag="OVW45_001833"
     CDS             complement(24982..25449)
                     /gene="mntR"
                     /locus_tag="OVW45_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCV5526300.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            25635..25763
                     /gene="mntS"
                     /locus_tag="OVW45_001834"
     CDS             25635..25763
                     /gene="mntS"
                     /locus_tag="OVW45_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCV5526301.1"
                     /translation="MNEFKKCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            26035..27618
                     /gene="opgE"
                     /locus_tag="OVW45_001835"
     CDS             26035..27618
                     /gene="opgE"
                     /locus_tag="OVW45_001835"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCV5526302.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(27667..28182)
                     /gene="ompX"
                     /locus_tag="OVW45_001836"
     CDS             complement(27667..28182)
                     /gene="ompX"
                     /locus_tag="OVW45_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCV5526303.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(28235..28300)
                     /locus_tag="OVW45_001837"
     CDS             complement(28235..28300)
                     /locus_tag="OVW45_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5526304.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            28535..29422
                     /gene="rhtA"
                     /locus_tag="OVW45_001838"
     CDS             28535..29422
                     /gene="rhtA"
                     /locus_tag="OVW45_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCV5526305.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            29740..30243
                     /gene="dps"
                     /locus_tag="OVW45_001839"
                     /gene_synonym="pexB"
     CDS             29740..30243
                     /gene="dps"
                     /locus_tag="OVW45_001839"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCV5526306.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            30647..31393
                     /gene="glnH"
                     /locus_tag="OVW45_001840"
     CDS             30647..31393
                     /gene="glnH"
                     /locus_tag="OVW45_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCV5526307.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            31532..32191
                     /gene="glnP"
                     /locus_tag="OVW45_001841"
     CDS             31532..32191
                     /gene="glnP"
                     /locus_tag="OVW45_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCV5526308.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            32188..32910
                     /gene="glnQ"
                     /locus_tag="OVW45_001842"
     CDS             32188..32910
                     /gene="glnQ"
                     /locus_tag="OVW45_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCV5526309.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            33027..35252
                     /gene="ybiO"
                     /locus_tag="OVW45_001843"
     CDS             33027..35252
                     /gene="ybiO"
                     /locus_tag="OVW45_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HCV5526310.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNVAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPTPGATPAEPLPPGEPT
                     L"
     gene            complement(35249..36175)
                     /gene="rlmF"
                     /locus_tag="OVW45_001844"
     CDS             complement(35249..36175)
                     /gene="rlmF"
                     /locus_tag="OVW45_001844"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCV5526311.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            36451..36711
                     /locus_tag="OVW45_001845"
     CDS             36451..36711
                     /locus_tag="OVW45_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCV5526312.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            36976..>37276
                     /locus_tag="OVW45_001846"
     CDS             36976..>37276
                     /locus_tag="OVW45_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCV5526313.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNAPG"
BASE COUNT         9043 a        10060 c         9339 g         8834 t
ORIGIN      
        1 caaatcgtga tctttgccgt tgatacgtag cgtaaaagag atacattttt tacccggacg
       61 atcaaagcaa ttgtatgccg gattccagct gttgttagag tcatctgaag ccaccgacca
      121 cggtcgtttc gatgggttaa aatcggcaaa aataacccgc cctgactcgt cgacaagcgg
      181 tccatttact tgttcctgat gataaacaac gattttatca ttcgccctcc gcgaaaagag
      241 ataatattta ttatcatcaa gtaccagtcc caccgagtct aaaaatggca tcatttccat
      301 tttcagaatc acggcgcgtt gaatctcctc tgtatttttt aataaataca gtgagtcctt
      361 taatgtcagg gccgaacgtt caatatcttt aaagaaatag ccaacgtttt tctgaataag
      421 ttcaaagcgg ctatcggcac gttttaccgc gcgttccgat accagttgat agttaatgat
      481 gaaaaaaaga ccaataaatg acagtgctgc caataaaaat aaaattatca gccttatctt
      541 tatcttttcg tataaactca gcatgttaat acatttaaca aaataactat ctgaacgctg
      601 tgccgctgca aaaatagtcc cttgctgcgg ctttttaacc aaattacccg attctacaaa
      661 atcagcagaa agttacaaca cttgcttagg gcaataattt cctgtgaggt gattaccctt
      721 tcaagcaata ttcaaacgta actatccttt aattttcgga tccagcgcat cgcgtaaacc
      781 atcgcccaac aaattgaacg ccagtacggt cagaaaaata gccagggccg gaaaaacagc
      841 gacatgcggc gcgataacca tatccgctcg tgcctcattg agcattgctc cccactctgg
      901 tgtcggcggc tgcgcaccga ggccgagaaa tgacaggctg gcggcagaga taatcgaggt
      961 gccaatgcgc atggtgaaaa acaccacgat agacgagacg gtcccaggca agatatgacg
     1021 caataaaatg gtcatatcgc tggcaccaat actgcgtgct gactcaataa aggtttgctg
     1081 tttcaacacc agcgtgttgc cgcgcaccag tcgggcaaac gcggggatgg aaaaaatggc
     1141 gactgcgata atcacgttag caatgccgct tcccaacacc gcaacaacag cgatcgccag
     1201 taaaataccc gggaaggcaa acagcacatc gcaaatgcgc atgatcagcc gatcccacca
     1261 gccttcataa tatccagcca acaagcccag caacgtcccg atcgccgcac cgataaacac
     1321 ggcaaacacg cccgccgcca gcgagatttg cgcaccaacc aggacacggc tgaaaatgtc
     1381 gcgccccagt gaatcgacgc caaaccagtg ctgcaaagaa ggtccgttat tcagattgtc
     1441 ataatcaaaa taattttcgg cgtcataggg agcgatccag cgtgcaaaaa tggccaccac
     1501 aatcaataaa ataacgaata atgcggcggt catcgccata tgctgacggc gaaatcgtcg
     1561 ccagaattca tgccacggtg tacgcacctg gtcaggtttg accagtggca tggcgtttaa
     1621 caccgcctga cgtcgccagt taaatagtcg catccttact tgtacctgat agccgggtta
     1681 atggcggcgt aaagcacatc caccactaag ttgataagaa taaattccag cgagaaaagc
     1741 agaatttccg cctgaatcac cgggtaatcg cgcatttcta cggagtcaac gagtaagcgt
     1801 ccaagtccgg gccagttgaa aactttctcc accacgatag aaccaccgag caaaaagcca
     1861 aactgtaagc ccatcatggt cactaccggg atcatcgcgt tacgtagccc gtgtttgagg
     1921 acaacccagg tttcgctcac ccctttcgcc ctcgcggtac gcatataatc ttcgcttaaa
     1981 acatcgacaa acgacgcgcg ggtaaagcgc gccatcacgg ctgccactgc cgcgccgagc
     2041 gtcagggagg gtaaaatgta gtgctgccaa ctgtctgctc ccacggtagg cagccagccc
     2101 agttcaacgg agaatacctg aattaaaagc atccccagcg caaatgccgg aaacgagatc
     2161 cccgacaccg caatggtcat actcaatcga tccggccaac gattacgcca gacggcggcg
     2221 ataattcccg ccgccatacc aaatatcacc gcccagacca tactggttat ggtcagccac
     2281 agcgttggca taaagcggct ggcaatctca tcggcaaccg gacgacgcga caccatcgac
     2341 aggccaaaat ccccctgcac agcattgctg atatagtgcc agaactggtg atacagcggc
     2401 tgatccaacc ccagctgctg acgcaccagt tctataacct gcgcatcagc ttcgagcccg
     2461 gcaatcaatc gcgccggatc gccgggcagc atatgcacaa ataaaaacac cagcaccgag
     2521 acgataaaca gcgtcggaat caaccccagt aagcgtttga taacgtaatt aagcattcca
     2581 ctccctgcgt tgcttattgc aaatccgcgt cttcaaagct gaagccggtg tctggcatga
     2641 tccaaaaacc ggtcaggttt ttactgtgtg ccgacaccag tttttctacc accagcggga
     2701 tccacggcga ctcttgccag atgatatcct gcgccgcctt atacaagcgg gtcttttccg
     2761 ccggatcatt agttttcagt gcctgagcca ggaagtcgtc cacctgttta ttgctgtaaa
     2821 atgcggtatt aaacagcgtc ggtggccagt tttgcgaggc gaacagtggc gatagcgccc
     2881 agtccgcttc gccggttgaa gccgaccagc cagtgtagaa catccgcacg ccgctctctt
     2941 tttgcccttt accttcaact tctgccgccc gctgtccggc atccatcgcg gtcacctggg
     3001 ctttaatccc gacctgcgct aactgctgct gggtaaattg cagcactttc tgcgcggtgc
     3061 tgtggttatg tgacgaccac agcgtggtac tgaaaccgtt gggatatccc gcctctttca
     3121 gtaattcgcg cgctttcact ggatcgtaag gccacggttt ataactttgc gcgtaggcga
     3181 tacttggcgg taccacacca gtagctggcg ttgcatagcc cgcaaaggca actttcacca
     3241 gcgccggacg gttaatggcg taattcagcg cctcacggac cttcgggtta tcgaacggct
     3301 tttgcgtcac gttcatactg atataacgct gcataattga cggactggcc atcaactcga
     3361 tatttttgtt tttctccagc agtgtggcct gctcgtaggg aatggggaaa gcaaactgcg
     3421 cttcaccggt ttgcagcatt gccgcgcggg tgttgttatc cgccaccgga cgccaggtta
     3481 tgctgtccag tttgggcaat cctggctgcc agtaacccgc gaatgttttc accttcacaa
     3541 aatcggtctg attccaggta tccagttcat acggtccggt tcccaccgga tgaaaaccaa
     3601 tctccttgcc atatttttcc agcgctgccg gtgaaatcat cgcggtcgcc ggatgggcaa
     3661 gaatattaat aaacgctgag aacggctgtt tgagggtaat ctttaccgtt gtcggatcga
     3721 tcgcctccgt tttggcaata ttcttataca ggttatagcg tttaagatga ttcgccggat
     3781 cgctggcccg gtccagattc gcttttaccg ccactgcgtt gaaatcggtg ccatcctgga
     3841 atttaatgcc ttcccgcaat ttcacggtgt aagtaatgcc gtcatcggaa acggtataac
     3901 tctccgccag cacgtttttc agtttcatct ctttatccag accgaacagc ccctggtaaa
     3961 acgatttcgc tacggcctga gataacgtgt cattcgcgtc atacggatcg agcgtggtga
     4021 aattcgatcc taccgccacc accacatctt tggcagcgaa tgcacaagat gccatcaacg
     4081 ctgtcgcaat gcccagcgcc actaacccac tacggtgtac agctcttgcc attttattct
     4141 ccgcctgaat gttatctacg catgaatgcg tattctgatt gtggttgtgc gacgtaatgc
     4201 cccggcccga cgcattgcaa cgagacggct gccacctctt cgccacgcag atgaatattg
     4261 ctgggaagat cgtccgacag cagtacacgc tgcggtcgtt gtcgggacgg ttcagcgacc
     4321 ggaactgccg ccagtaattt acgcgtataa ggatgctgcg ggttttcgaa gaccgcgcgc
     4381 cgtggaccaa tttcaacaat ttgcccgaga tacatcaccg ccacacgatg actaatccgc
     4441 tctaccacgg ccatatcgtg ggagataaac agatacgcaa tgccgaaatc acgctggaga
     4501 tcgagcaaca agttgataat ctgcccgcga atagaaacat ccagcgccga aacggcttcg
     4561 tcggcaatga tcacttttgg attcaatgcc aacgcgcgag caatgcagat gcgctggcgc
     4621 tgaccgccgg aaaactcatg cgggtaacgc caggcatgtt caggtaacag gcccacgcgc
     4681 tccagcaacc acgcaacgcg tgcagccgcg tctttacctg gcaataaacc gtgtacacgc
     4741 agcggttcga taatcgaatc accgatggtc tgacgtgggt ccagcgaagc gtaagggtcc
     4801 tgaaaaataa actgaatatc ccggcgcaat gcctgaagtt tgccgggtga caaggtatcg
     4861 attctctgac cgttaaagat aatttcgccg ccctgcgatt cgaccaggcg cagcaacgcc
     4921 cgcccggtag tggatttacc gctgccagac tcgcccacca gcgatagcgt ttcgccaggc
     4981 cagagatcaa aactgacttt ctcaacggca tgcacttccc gcgttacgcg attcaacaaa
     5041 ccgctgcgca aagggaaacg ggtgacaaga ttacgcactc gtaaaacagg ttcgccatcc
     5101 accaccgttt tctgctcgat ggggggggcc tgtttcgctg gatgttcaag cgatatcaac
     5161 gggaaacgtc ggggataatc caaccctttc atcgcaccaa gttgcggaac agcagctaac
     5221 agcgcacggg tgtaaggatg ttgcggtgca tgaaaaatct gttcgacggt acccgtttcc
     5281 accgcctcgc cctgatacat caccagtacc cgatcggcaa tctctgccac cacgcccata
     5341 tcgtgagtga taaagataac gcccatcgac atctcttttt gcaatacttt gattaattgc
     5401 aggatctgcg cctgaatagt gacatccagc gcggtggttg gctcatcggc aatcagcacc
     5461 gccgggcggc atgacagcgc catcgcaatc atcactcgct ggcgcatccc gccagagagt
     5521 tgatgcggat aacgtgaaag aatggtttgt gcctcaggaa tgcgtacctg atccagcatc
     5581 cgcttcgcct cgaccatcgc ttcttcacga ctggcgttct gatgcagacg aattgattcg
     5641 gcaatctgtt cacccacagt aaataccggg ttcagcgatg tcatcggctc ctgaaatatc
     5701 atcgccatat ccgcaccgcg aacatggcgc atttgtgcag cgctctgctc gctaagttca
     5761 atcacttcgc gactgcgccg ctgcaacagc attttatcgc actgtactaa accgcccgcc
     5821 tgttccaaca ggcgcatcaa tgccaacgca gtcactgact taccggagcc ggattcgcca
     5881 acaattgcca gcgtctcacc gcgttgcaga ctaaaagaga gattgcggac cgcagctatt
     5941 ttctgctggt cctgcataaa ggcaatattc agattttcaa ccgccagcac attaccggca
     6001 tcaagttcat cactgtgtgg caacggtgtc ccctttttca cggtagatac cggtggttgg
     6061 cgtatcgcct gcgtagcccc aggcgcgata cattccttcg gtgttaaacg gtagcgcgac
     6121 attcccttca tggtcgatag cgattaagcc accgctaccg ccaagcgcag ggagtttttc
     6181 cattactacc cgctcgcagg cttccgcgag acttaatccg ccgtaatcca ttaacgcggc
     6241 gatgtcatat gccgccagcg cgcggatgaa gacttcgccc gtgccgatac aagaaaccgc
     6301 cacactggcg ttattggcgt agcatccggc acccactaag ggactatcgc caactcgtcc
     6361 gggtaattta ttggtcattc cgcctgtgga cgtggctgcc gccaaattgc cgtctaaatc
     6421 caacgccacg gcccccacgg tgcccatttt ctgtttttca tccagtggcg caccgctatg
     6481 gtcgaggacg gttgcccctt ccttgcgcgc tgccagtagt tgttcataac gcaaaggcgt
     6541 ggagaaaatc tccggcgaga cgcgctccat gccacgagca aacgcaaaat tttctgcccc
     6601 ttcgccaatc atcatcacat gcgggctttg ctccatcacc agccgggcgg caagaaccgg
     6661 attacgcaga tgactaacgc ccgccaccgc accggctttc agggtgttac catccatcac
     6721 acaggcgtcc agttcatggg tttcatcacg cgtaaagaca gcgccaattc cggcgttaaa
     6781 cagtggacac tcttccagca gacgcaccgc ttccgtcacc acatccagcg cactttcgcc
     6841 cgcttccagc attttctgcc cggtttcaac aatggcagac aacgcctcaa tgtagcgtaa
     6901 ttcctgttgc agactcatct gcgcgcggct aattgcacct gcgccaccat gaattgcaat
     6961 gactgctttg cccatagtat tcgtccatta agggtggctc gccacttttg ctataaatat
     7021 cagaattttt atgaattact cattcaattt ttgaatatag aaagatggat atggcatgta
     7081 aaggcaggag ccaacgctac gcccgaggtg gcatacagtc tgtggcgttt ttctgacata
     7141 ataggcaaat tcgattttgc ctccgcagga gtgttttcat ggaatttacc accggattga
     7201 tgtcgctcga caccgcgctt aatgagatgc tttctcgcgt caccccactg accgcccagg
     7261 aaacgctgcc actggtacag tgttttggtc gtattctggc gagcgatgtc gtttcgccgc
     7321 ttgatgttcc ggggtttgat aactccgcaa tggacggcta cgcggtgcgt ttagccgata
     7381 ttgcctccgg gcaaccgctg cccgttgccg gtaaatcctt tgccggtcag ccgtatcatg
     7441 gtgaatggcc tgcgggcacc tgcattcgta ttatgaccgg tgcgccggtg ccggaaggct
     7501 gcgaagcggt ggtgatgcag gagcagactg aacaaacgga caatggcgtg cgttttactg
     7561 ctgaagtgcg tagcgggcaa aatattcgcc gtcgcggtga agatatctct gcaggtgcgg
     7621 ttgttttccc ggcgggaact cgcctgacta ccgcagagct gccagtaatt gcttcactgg
     7681 ggattgccga agttccggtg attcgtaaag tgcgtgtagc gcttttttct accggtgatg
     7741 aactccagtt gcccggtcag ccgctgggcg acggccaaat ctacgacacc aaccgtctcg
     7801 ccgtacacct gatgttagaa cagctgggtt gcgaggtaat caacttaggg attatccgcg
     7861 acgatcccca tgccctgcgc gccgcattta ttgaagccga cagccaggcg gatgtagtga
     7921 tcagttccgg cggtgtttca gtgggtgaag cggattacac caaaacgatt cttgaagagc
     7981 tgggggagat cgccttctgg aagctggcga ttaaaccagg taaaccgttc gcgttcggta
     8041 aactcagcaa tagctggttc tgcggcctgc cgggcaaccc ggtttcagcg acgctgacct
     8101 tctatcaact ggtacagcct ttgctggcaa aactaagcgg caacaccgcc agcggcctgc
     8161 ccgcgcgcca gcgcgtacgc acagcgtccc gcctgaaaaa aacgccagga cgtcttgatt
     8221 tccagcgcgg cgtgctgcaa cgcaacgccg acggtgaact tgaagtgacg accaccggac
     8281 atcagggttc acatatattt agctccttta gtctcggcaa ctgctttatc gtgctggaac
     8341 gcgatcgcgg caatgtggac gtgggcgaat gggtggaagt agaaccgttt aacgcgttgt
     8401 tcggaggcct gtaatggcgg aactcagcga tcaggagatg ctgcgctaca accggcaaat
     8461 cattctgcgc ggttttgatt ttgacggtca ggaggcgcta aaagattctc gtgtgctgat
     8521 cgtcggcctc ggtggactcg gctgcgcagc ttcgctgtat ctggcaagcg ccggtgtcgg
     8581 caacctgacg ctgctcgact tcgacacggt ttcgctctcg aatctgcaac gccagacact
     8641 gcacagtgat gccacggtcg ggcaaccgaa ggtggaatcc gcccgtgacg ccctgacgcg
     8701 gatcaaccca catatcgcga ttacgccagt caatgcactg ctagatgacg cagaacttgc
     8761 agcattgatt gctgaacacg atctggtgct cgactgtacg gataacgttg cggtacgtaa
     8821 tcaactgaac gcaggctgtt ttgccgcgaa ggtgccgctg gtttccggcg cggcaattcg
     8881 tatggaaggg caaatcacgg tcttcactta tcaggacggc gaaccgtgct atcgctgcct
     8941 tagccgtttg tttggcgaga acgcattaac ctgcgtagaa gcaggcgtga tggcaccgtt
     9001 gatcggcgta attggttcgt tgcaggcaat ggaagcgatc aaactgctgg caggttatgg
     9061 caaaccgacc agcgggaaaa tcgtcatgta cgatgcgatg acctgtcagt ttcgcgaaat
     9121 gaaactgatg cgcaatccgg ggtgtgaggt gtgcgggcag taattgccac ctccaggcct
     9181 gataagacgc gacaagcgtc gcatcaggca tttgctccag acgccagatg cggcgtgaac
     9241 gccttatctg gcctacggtt cgggcacaga tttccaagcg cggcaggcat cggtgcactc
     9301 cgccacgtca tcagcaattt atgaggatgc agaacgtccg gttaaatcga cgttctgcca
     9361 aacgctccct gccagtcctg ctcaaacttc gccacagcgg cttccaccgc cggatagcta
     9421 atcatctgtt gtgccacatc cagtggcaga gtaattgatt cacatcctgc cagtaagcag
     9481 tccagcgcct gacgcggggt tttgaaactc gctgccagca ctttcgcctg cggcgcatgc
     9541 attttcaata actggtgtaa gtcggtcaca gtctgaatgc cgctaccgcc ctgagcatca
     9601 atacgattaa cgtaaggcgc aacatattcc gcacctgcca gcgccgacag cagcccttgt
     9661 gctgcgccat ataccgcggt tcccagcgtt ggaatccctt ccgcttttaa catcttaata
     9721 gctgccagcc cctcggcggt caccggaact ttcaccacga tatccgcaat aatagaacgc
     9781 aatttacgtg cgtcattcac catcccttca gcagtggtag ccattacctg ggcaaacaga
     9841 cgcccctgac cgcccatcgc ttcatgaagt tgcggaagca caacatccag cggtttttta
     9901 cccgcggcga taatgcttgg gttagtggtc acacccgcca gcggaaaaat acgtgacagc
     9961 gccttcaccg caacaacgtc tgaagtatcc agatacagtt ccataaccat cctcaaaatg
    10021 tactttttgt ctgaatgtag actatcacgg gaaaactctc tgaacagttg acgcacatca
    10081 atctaacttt cattcgaaag tattttaatg tttgaacgaa acagagaggc aactatgatt
    10141 ttcaatattc agcgctactc gacccatgat ggccccggta tccgcacggt cgtatttctt
    10201 aaaggctgtt cgctgggctg ccgctggtgt cagaacccgg aaagccgcgc ccgcacgcag
    10261 gatctgctgt atgacgcacg actgtgtctg gaaggctgcg agctgtgcgc taaggccgcg
    10321 ccggaagtga ttgagcgcgc gctgaatggt ttgcttattc atcgggaaaa gttaaccccg
    10381 gagcatctga cggcgttaac cgactgctgt ccgacacagg cattaaccgt gtgtggtgaa
    10441 gtgaaaagcg ttgaggagat catgacgacc gttctgcgcg ataaaccgtt ttacgatcgc
    10501 agcggcggcg gtttaacgct ttcgggtggt gagcccttta tgcagccgga aatggcgatg
    10561 gcgctactgc aagccagcca cgaggcaggc attcatactg cggtagaaac ctgtctgcat
    10621 gtgccgtgga aatatatcgc cccttctctg ccctatatcg atctgtttct tgccgattta
    10681 aaacacgttg ccgacgcgcc gtttaaacag tggaccgacg gtaacgccgc cagagtgctg
    10741 gataacctga aaaaactcgc cgcagcgggc aaaaaaatca ttatccgcgt gccgctgatt
    10801 cagggcttta atgccgacga aacctctgta aaagctatta ccgattttgc cgccgacgag
    10861 ctgcacgttg gcgaaattca tttcctgccc taccacacgc taggcatcaa caaatatcac
    10921 ttacttaatc tgccctatga cgccccggaa aaaccgcttg atgcgccaga actgctcgac
    10981 tttgcccagc agtatgcctg ccagaaaggg ttaaccgcga ccttacgagg ataacaacca
    11041 tgaccacact gaaactggac acgctcagcg accgcattaa agcgcacaaa aatgcgctgg
    11101 tgcatattgt gaaaccgcca gtctgtaccg agcgcgcgca gcactatacc gagatgtatc
    11161 aacaacatct cgataagccg atcccggtac gtcgcgcgct ggcactggcg catcacctgg
    11221 cgaatcgcac catctggatc aaacacgatg agttgatcat tggcaaccag gcaagcgaag
    11281 ttcgcgccgc gccgatcttc ccggaatata ctgtctcgtg gatcgaaaaa gagattgatg
    11341 atctggcaga tcgtcccggt gctggctttg cggtgagcga agagaacaaa cgcgttctgc
    11401 atgaagtgtg cccgtggtgg cgcggtcaga ccgtacagga tcgctgctac ggcatgttta
    11461 ccgatgagca aaaaggtctg ctggcgaccg gaatcattaa agcggaaggc aatatgacct
    11521 ccggcgatgc gcacctggcg gtgaatttcc cgctgctgct ggaaaaaggt cttgatggtc
    11581 tgcgcgagaa agtagcggaa cgtcgctcgc gcatcaatct gacggtactg gaagatttgc
    11641 acggcgaaca gttcctgaaa gcgattgata tcgtgctggt ggcagtcagc gaacacattg
    11701 aacgttttgc agccctggcg cgtgaaatgg ccgcgaccga aacccgcgaa agccgtcgcg
    11761 atgaactgct ggcgatagca gaaaactgcg atcttatcgc ccaccagccg ccgcagactt
    11821 tctggcaggc gctgcaactg tgttacttca tccaattgat attgcagatc gaatctaacg
    11881 gtcactcggt atcgtttggt cgtatggacc agtatctcta cccgtactat cgccgcgacg
    11941 ttgaactcaa ccagacactg gatcgcgaac acgccatcga attgctgcat agctgctggc
    12001 tgaaactgct ggaagtgaac aagatccgct ccggctcaca ctcaaaagcc tctgcgggaa
    12061 gtcctctgta tcagaacgtc accattggcg gacaaactct ggttgatggt caaccaatgg
    12121 acgcggtgaa tccactctct tacgctatcc tcgaatcctg cggtcgcctg cgttccactc
    12181 agcctaacct cagcgtgcgt taccatgcag gaatgagtaa tgatttcctc gacgcctgcg
    12241 tacaggtgat ccgttgcggc ttcgggatgc cggcgttcaa caacgacgaa atcgtgatcc
    12301 cggaatttat taaactcggt attgaaccgc aggacgctta cgactacgca gcgattggtt
    12361 gtatcgaaac cgccgtcggt ggcaaatggg gctatcgctg taccggcatg agctttatca
    12421 acttcgcccg cgtgatgctg gcggcgctgg aaggcgggcg tgatgccacc agcggcaaag
    12481 tgttcctgcc acaagaaaaa gcgttgtcgg caggcaactt taacaatttc gatgaagtaa
    12541 tggacgcgtg ggatacgcaa atccgttatt acacgcgtaa atcaatcgaa atcgaatatg
    12601 tcgtcgacac catgctggaa gagaacgtgc acgatattct ctgctcggcg ctggtggatg
    12661 actgtattga gcgagcgaaa agtatcaagc aaggcggcgc gaaatatgac tgggtttctg
    12721 gcctgcaggt tggcattgcc aacctcggca acagcctggc ggcagtgaag aaactggtat
    12781 ttgaacaggg cgcgattggt cagcaacagc tggctgccgc actggcggat gacttcgatg
    12841 gcctgactca tgagcaactg cgtcagcggc tgattaacgg tgcgccgaag tacggcaacg
    12901 acgatgatac cgtcgatacg ctgctggctc gcgcttatca gacctatatc gacgaactga
    12961 aacagtacca taacccgcgc tacggtcgtg gtccggttgg cggcaactat tacgcgggta
    13021 cctcgtctat ctccgctaac gtaccgtttg gcgcgcaaac tatggcaaca ccggacgggc
    13081 gtaaagccca caccccgctg gcagaaggcg caagcccggc ctccggtact gaccatcttg
    13141 gccctactgc ggtcattggc tcagtgggta aactgcctac ggcagcgatt ctcggcggcg
    13201 tgttgctcaa ccagaaactg aatccggcaa cgctggagaa cgaatctgac aagcagaaac
    13261 tgatgatcct gctgcgtacc ttctttgaag tgcataaagg ctggcatatt cagtacaaca
    13321 tcgtttcccg cgaaacgctg ctggaggcga aaaaacatcc ggatcagtac cgcgatctgg
    13381 tagtgcgtgt cgcgggctat tccgccttct tcaccgcgct ctctccagac gcacaggacg
    13441 atatcatcgc ccgtactgaa catatgctgt aatctcctcg gcccggcggc gtcgtcgccg
    13501 ggctaaatca ttcacatcat caatttcatc cttactttca ttcgaaatat aatttgtgct
    13561 ctgcgtcaca ttgttattac actgttacgg tttcattttc gagccaggag cacacctatg
    13621 agcgtaaaag ttatcgtcac agacatggac ggtacttttc ttaacgacgc caaaacgtac
    13681 aaccaaccac gttttatggc gcaatatcag gaactgaaaa agcgcggcat taagttcgtt
    13741 gtcgccagcg gtaatcagta ttaccagctt atttcgttct ttcctgagct aaaggatgag
    13801 atctcttttg tcgcggaaaa cggcgcactg gtttacgaac atggcaagca actgttccac
    13861 ggcgaactga cccgacatga atcgcggatt gttattggcg agttgctaaa agataagcaa
    13921 ctcaattttg tcgcctgcgg tctgcaaagt gcatatgtca gcgaaaatgc ccccgaagca
    13981 tttgtcgcac tgatggcaaa acactaccat cgcctgaaag ctgtaaaaga ttatcaggag
    14041 attgacgacg tactgttcaa gttttcgctc aacctgccag atgaacaaat cccgttagtg
    14101 atcgacaaac tgcacatagc gctcgatggc attatgaaac ccgtcaccag tggttttggc
    14161 tttatcgacc tgattattcc cggtctacat aaagcaaacg gtatttcgcg gttactgaaa
    14221 cgctgggatc tgtcaccgca aaatgtggta gcgattggcg acagcggtaa cgatgcggag
    14281 atgctgaaaa tggcgcgtta ttcctttgcg atgggcaatg ctgcggaaaa cattaaacaa
    14341 atcgcccgtt acgctaccga tgataataat catgaaggcg cgctgaatgt gattcaggcg
    14401 gtgctggata acacatcccc ttttaacagc tgacctctct accggagccg tgcgctccgg
    14461 ttttcccttc tttttacatc ctgtacatca catcaaatca ttaaacttgc attaacttat
    14521 ttataactta tattgtcgca tgcaaatcag aatggtttcg aatgaaagtt aatgagaggt
    14581 ggttatggct tctactttta ccagcgacac attgcctgcc gatcacaaag cagctatccg
    14641 tcagatgaag cacgcgctgc gggcgcagct tggcgacgtc cagcagatct ttaatcagct
    14701 aagcgatgac attgccacgc gagtggctga aatcaacgca ctcaaagcac agggcgatgc
    14761 cgtctggccg gtgctgtctt atgccgatat caaagcaggt catgttactg cagagcagcg
    14821 cgaacagatt aaacgtcgcg gttgtgcggt gataaaaggc catttccccc gcgaacaagc
    14881 gctaggctgg gatcagtcga tgctggacta tctggaccgc aaccgctttg acgaggtcta
    14941 caaaggcccc ggcgataatt tcttcgggac gctcagcgct tcacgtcccg agatttaccc
    15001 catctactgg tcgcaggcgc aaatgcaggc ccgccagagt gaagaaatgg cgaatgcgca
    15061 gtcgtttctc aatcgtctgt ggacatttga aagtgatgga aagcaatggt ttaacccgga
    15121 tgtgagcgtc atctaccctg accgtatccg ccgccgtccg cccggaacga cctccaaagg
    15181 tcttggagcg cataccgact ccggggcgct ggaacgctgg ctgcttccag cgtatcagcg
    15241 cgttttcgcc aacgtcttta atggcaatct ggcgcaatat gatccctggc atgcggcaca
    15301 tcgtacggaa gttgaagagt acacggtgga caacaccacc aaatgttccg tgtttcggac
    15361 attccaaggc tggacagcgc tctctaatat gctgcctggt caggggctgc tgcacgttgt
    15421 gcccattcct gaagccatgg cgtacgtact gttacgtccg ctgcttgatg atgtgccgga
    15481 ggatgaactg tgcggcgtag cgcccggaag agtgttgccg gtatcagagc aatggcatcc
    15541 actgttaatt gaggcgttaa ccagcattcc aaaactcgag gccggagact ccgtctggtg
    15601 gcactgcgac gtcatccatt ccgttgcccc cgttgaaaat caacagggtt ggggcaacgt
    15661 gatgtacatt cctgcggcac caatgtgcga gaaaaatctt gcctacgcgc acaaggtgaa
    15721 ggccgcactg gaaaaaggcg catcgccggg cgacttcccg cgcgaggact atgaaacaaa
    15781 ctgggaagga cgctttacgc ttgccgacct caacattcac ggtaagcgag cgttgggaat
    15841 cgacagctga gtgtcgtaca gcccttcccg ttccacggcg gtgttccctt ttcctgggcg
    15901 aacccgccgc actgactccc gcaccgccaa tcgtccgtgc agcagcagcg agcccaccgg
    15961 agcatccggg cgaatcagcc gctgttgcaa catttgcacc gcttcattgc ctagctcatc
    16021 gcgtggcaca tgcacagccg tcaggggaac atcctcgatt gccgcaaggt taaagccgtc
    16081 gatactcatc accgagacat cctgtggtac gcgcagacca cgacgatgta gcgcatttac
    16141 cgttcccgcc gccataaaat ccccgccgac cagaaacgcc gtcggcaacg tcttgccatt
    16201 ggcggcatcc agccacgctc cgaccttttc ttccgcttct ttagcgctaa aactgctgat
    16261 attcagcaaa tgacggtcat ggtgaaaggc cagattatgc tgacgccatg attctttaat
    16321 ccctgcaaac cgctgctcca tagtgtagcg gcgcaggcac atcacattca tcacctcccg
    16381 gtgacccatt tcaaacagat agcgggcggc gaacgcgcca attaaccgat gatccggcgc
    16441 aatcgccggg aggcgcattc gttcatcacg gcagttgatc agcacacaag gcttagcgag
    16501 atcggcggcc agatcatgga tgtgcggatc gtcaatcccc agcaggatcg ctgcctgcgt
    16561 atctgcttca ttcatccgcg agagaaagag cgcgggatcg ctgtcaaact cctccagcgc
    16621 gcagtaacgc agccgcactt catgcggcga taacgctttg ctgatgctct ggatcacacg
    16681 gtaataaaag atatcagatc gttggtcaaa agcgcgttgc ggcgcgaaaa taatcaggtt
    16741 gttcagcaac atacgacctg ctgccatgcc atccatcacg cccaactcac gcgcgcaggc
    16801 cagcacctgt tcgcgagcgc gtgcactggt attcgctttg ccagccagca cccgcgaaac
    16861 cgtactcggc gatagcccgg tacgtgcggc gatatcggcg attttcagct tcttgtccat
    16921 tttgtgatct gcctctaatt cggaaatgac agaattttca tgagcaaaaa agcccattct
    16981 gtctggcatt aaatccattt cagtaatgta tatcagtcac tcatggaaaa acgtgcacaa
    17041 aacccgcttt ctttcgcttt tagggtctct tactgtgaat tggtagaaca acttccatac
    17101 aaaaataatg gaaaaacgaa caataaagac tttatttatc aaaaagttaa aagtggtgta
    17161 acaactccat tctgacgttg ccgcacaaac cctataccat tctgtggagt gcatatgagt
    17221 cagggtatca ataacgatat gactgccagt aaaccgcgcc atatcgtcaa aaatctgcgt
    17281 tggtggatac tggtcctttt tttgctcggt gttactgtca actacatcac gcgtaactcg
    17341 ctgggtattc tcgcccccga actgaaaagc agcctaggga ttaccaccga acaatattcc
    17401 tggattgtag gtgctttcca gctcgcctat accctattcc aaccgttgtg cggatggctg
    17461 attgacgtca ttggcctcaa actgggcttt atgatctgcg cgggactgtg ggcactggcc
    17521 tgtctgatgc atgcgggggc gggaaactgg gtacatctgg cgctgttgcg cttctttatg
    17581 ggcggtgcgg aagccgcggc aaccccagca aacgccaaaa ccatcggtga atggtttcca
    17641 aaatcagaac gcccgatcgc cgcaggctgg gcaggcgtcg gtttttccat cggtgccatg
    17701 ctggccccac cgattatcta ttttgcccac gcttcattca gctggcaggg tgcatttatg
    17761 ttcactggca tcctggcgat gctgtgggtg gtgctgtggt gggcgttcta tcacagcccg
    17821 gataagcatc ctaacctcag caaagccgag ctggacttta ttcgccagga taacgaagcg
    17881 cccccggtca agttaccctt tctgaccgca ctgaaaaccg tgggcaaaaa taaacgcttt
    17941 tacggtatcg ctatcccggc ctttatggca gaaccggcgt gggcggtgat gagtttctgg
    18001 gtgcctctat acctgtccaa aacctatggc atggacctga agcagatcgc attattcgcc
    18061 tggttgccgt tcctcgccgc tgatcttggc agtattgcca gcggctatct gaccaaactt
    18121 tacacccgcc tgtttgggct gagtcgtgtg aactccgttg tcgcatcatc ggtcagtggc
    18181 gcattcttga tggtttcgct ggcggtgatg gcctttacca ataacccgta tatcgccatc
    18241 gtccttatct ccatcggcgg cttcggtcac cagatcatct cctgcatgct gagcgcactg
    18301 gtggtggaat ccttcgacaa aggtcagatg gccactgtta acggcatgcg cggttctgcc
    18361 gcgtggatcg caagtttcct gttctccctg attatcgggg ttaccgctga caaaatcggc
    18421 tttaaccccc tgtttatcgc catgggcttc tttgacctga ttggcgcttt cttcctggtc
    18481 acctttattg ctgaacgtcg cgcaaaacgc gcctgagagt ggatgtaaac atgaaaacgc
    18541 tgaaaaactg gaccctgcac aaacagtctg cccaccacgt tgaactgctg gtcgatggcc
    18601 agcatatcct gtgcctgtac gtgctggaag aaaatctgtt ccgcgtgctg ttaaaacgca
    18661 aaggcgagct ggcgctagat cgcacctgga gcattgctcc ggcgcaggat gtgccatggg
    18721 aagggcgcag ccgcgaagat ctcagcggct ttagcctgcc cgcgtggaca ttgatccagc
    18781 acgacgacac cgtggttatc gccaccgagt cactgcgtgc aaccgttcac cagccgctgt
    18841 ggctggagtg gcactatcgt aacaacgaag gcgaatggct gccgttggtg aatgaccgcc
    18901 cgaccagcgc ttatctgctg aatgcccacg gcgatggcgt ggcacactat cagagccgtc
    18961 gtaaagatga acgcttttat ggcctcggag ataaagccgg cgatctgcaa cgcaacggta
    19021 aacgttacga gatgcgtaac ctcgacgcga tgggctacaa cgccgttagt actgacccgc
    19081 tgtacaaaca cattccattc accatcaccc gccgcgatga cattagcttc ggcctgtttt
    19141 atgacaacct cagcagctgc tggctggatc tgggcaacga gatcgacaac taccacaccg
    19201 cctatcgccg ctggcaggcg gaagcgggtg atatcgacta ctacctgttt accggcgaac
    19261 gcgtacttga tatcaccaaa gccttcgtac gcctgaccgg aaaaaccctg ttcggaccga
    19321 aatggagcct gggctacagc ggttcaacta tgcactacac cgatgcgccg gacgcacaaa
    19381 atcagttgat gaactttatc cgcctgtgtg aagaacatgc aattccctgc gattcgttcc
    19441 agctctcttc cggttatacc tcaattaacg gcaaacgcta tgtgttcaac tggaactacg
    19501 acaaagtgcc gcagccgaaa gtgatgagcc aggcatttca caatgctggt ttgcacctgg
    19561 ccgccaatat caagccgtgc ctgttgcagg atcacccacg ctataacgaa gtggcagaaa
    19621 gaggactgtt catccgtgat tcagaaacag atgaaccaga acgttccagc ttctgggatg
    19681 acgaaggttc taacctcgac tttactaatc cgcagaccat ccagtggtgg caggaaggcg
    19741 tgaccacaca gcttctggag atgggtatcg actccacctg gaacgacaac aacgaattcg
    19801 aagtatggga cggtgaagca cgctgtcacg gctttggtca gacgatcgcg atcaaacata
    19861 ttcgcccagt gatgccactg ctgatgatgc gagcctcgat ggaagcccag cagcgtttcg
    19921 ctccggaaaa acgtccgtac ttgatctccc gctccggctg cgcgggaatg cagcgctacg
    19981 tgcagacctg gagtggcgat aaccgcacta gctgggacac tctgcgctac aacacccgca
    20041 tgggtctggg gatgagcctc tccgggctgt ataacctcgg tcacgacgtc ggcggtttct
    20101 ccggtgataa gccggacccg gaactgtttg tacgctgggt acaaaacggc gtgatgcatc
    20161 cgcgctttac gattcactca tggaacgatg accataccgt aaacgaaccg tggatgtatc
    20221 cgggcgtcac tccggcgatc cgcagcgcta ttgagttgcg ctatcgcctg ctgccctact
    20281 tttataccct gatgtggcag gcatacgccg atgatgaacc aatgctacgt ccgactttcc
    20341 ttgaccacga gcacgacgtt cagacttttg aagagtgtga tgacttcctg cttggtcgcg
    20401 atattttggt tgccagcgtc gtagagcaag gcgagcgcca gcgccgtgta tggctgccag
    20461 ataacgaaac tggctggtac gatttctaca acggcgaatg gttcagtggc ggtcagtgga
    20521 tcaccctcga cgcaccgctg gagaaactgc cgctgatggt tcgtgccggg gctggtctac
    20581 cgctcagcaa acgcatcacc tatgtgagcg cagaacaaga cgatacccgc gaactgaaac
    20641 tgttcccgct gaaaggtgtg ggcacaacgt ccggcctgct gtttgaagac gatggcgaaa
    20701 gctggggcta ccaaactggt aatgcgcttt gggtggaatg ggaaatggtt tgcgatgggg
    20761 cgaccatcaa cctgaaagtc aacgcgcgcg gggattatcg tcctgcatgg agtgcactga
    20821 aagtgtcatt accattggaa gaaaaacgca cgctgctggt caatggtgtt gaagggagtg
    20881 aatggatgcg ttaaatcatt accggggccg ggaggcgact gcaccttacc cggtccagcc
    20941 atcgaatcct ttacctcagt atttcacgct gcaggaaggc agcaaacctg taaaccccgg
    21001 gagcttaaat aagtaagtga ccggggtgaa caggtgaagc caatgcatct gccgcatgaa
    21061 agacggcggg gaattaagcg tcgatccctt tactacgcag gtaatcttcg taattaccgc
    21121 taaagtcgat cgcgcgttcc ggggtgattt ccagaatgcg ggtcgccagg gagcttacga
    21181 actcacggtc gtgtgaaaca aagatcagcg tgccctgata cagttccagc gccatgttca
    21241 gtgactcgat ggattccata tctaggtggt tggtcggttc gtccatgatc agaatgttcg
    21301 gcttctgcat cattaactta ccaaacagca tccgcccttt ttccccaccg gaaagcactt
    21361 tagctggctt tttgatgtcg tcctggctga acagcaaacg accgagaatg ctgcgtaccg
    21421 cctgctcgtc atcgccttcc tgcttccact gactcatcca ttcgaacacg gtcagatcat
    21481 tttcaaactc atattcgtgg tcctgggcgt agtaaccaat gcgcgcgttt tcagaccatt
    21541 ttacggtgcc gctgtccggt tgcagatcgc ccaccagcgt tttcagcagc gttgatttac
    21601 cgacgccgtt ggtacccagt accgcaagtt tttcaccgac ctccagcagc aggttgagat
    21661 ttttaaacag tggaccgtta tcaaaccctt tggtcagacc ttccacttcc agcgcgttac
    21721 ggaacagttt cttatcctgt tcaaaacgga tgaacgggtt ctgacggctg gaggctttca
    21781 cctcttccag tttgatttta tcaatctggc gcgcgcgcga agttgcctgg cgagatttcg
    21841 aggcgttggc gctaaagcgg ctaacgaaag attgcaactc agcaatctgc gctttcttct
    21901 tggcgttatc ggccagcaga cgttcacgcg cctgagtcgc cgccgtcatg tactcatcgt
    21961 agttacccgg ataaacgcgc agctcgccgt aatccagatc cgccatgtgg gtacagacca
    22021 tgttaaggaa gtgacggtcg tgcgagatga tgatcatggt gctgtcacgc tcgttcagca
    22081 cctgttccag ccagcgaatg gtgtcgatgt cgaggttgtt ggtcggttcg tcgagcagga
    22141 gaatatccgg atcagcaaac agcgcctgcg ccagaagcac acgcagtttc cagccaggag
    22201 caacttcact catcgggccg tagtgctgtt ccactggaat tcccacgcca agcaacagtt
    22261 caccggcgcg agcttccgca gagtaaccgt ccatttcgcc gtatttaact tccagatcgg
    22321 ccactttata gccgtcttcc tcactcattt ccggcaaagc atagatgcgg tcacgctcct
    22381 gcttcacttc ccacaactct ttatgcccca tgatcaccgt atccagcaca gtgaactctt
    22441 caaaggcaaa ctgatcctga cgcagcttac caatgcgctc gttgggatcg agggaaacgt
    22501 tacccaaagt cggctctaag tcgccgccga ggatcttcat aaaggtggat ttaccactac
    22561 cgttcgcgcc aatcaggccg taacggttgc cgccgccgaa tttgacggaa atgttttcaa
    22621 acaacggctt actgccgaac tgcatggtga cgttactgga aactaacaca ggcgtatcct
    22681 gaaaagagat atgacaaacc gcgcattatg ccataagtct gaaagaagat cctgctcctc
    22741 gcttaaggtc tacactcaag ccaaaggcaa aaaatgtgat ttcgtacaca tctgatttca
    22801 ctgtgagctg gaatgaactt ataatgcgct tccaatactc taatattctc aacccaatgg
    22861 cctgccaggc acaaaatctc gcttaacatg aatatgaaat tgaaaacatt attcgcagcg
    22921 gccttcgctg ttgtcggctt ttgcagtacc gcctctgcgg taacttatcc tctgccaacc
    22981 gacgggagtc gcctggttgg tcagaatcag gtgatcacca ttcctgaagg taacactcag
    23041 ccgctggagt attttgccgc ggagtaccag atggggcttt ccaatatgat ggaagcgaac
    23101 ccgggtgtgg ataccttcct gccgaaaggc ggtactgtac tgaacattcc gcagcagctg
    23161 atcctgccgg ataccgttca tgaaggcatc gtcattaaca gtgcagagat gcgtctgtat
    23221 tactatccga aagggaccaa caccgttatc gtgctgccga tcggcattgg tcagttaggc
    23281 aaagatacgc ctatcaactg gaccaccaaa gttgagcgta agaaagcagg cccgacctgg
    23341 acgccgaccg ccaaaatgca cgcagagtac cgcgctgcgg gcgaaccgct tccggctgtc
    23401 gttccggcag gtccggataa cccgatgggg ctgtatgcac tctacatcgg tcgcctgtac
    23461 gctatccatg gcaccaacgc caacttcggt atcggcctgc gtgtaagtca tggttgtgtg
    23521 cgtctgcgta acgaagacat caaattcctg ttcgagaaag taccggtcgg tacccgcgta
    23581 cagtttattg atgagccggt aaaagcgacc accgagccag acggcagccg ttatattgaa
    23641 gtccataacc cgctgtctac caccgaagcc cagtttgaag gtcaggaaat tgtgccaatt
    23701 accctgacga agagcgtgca aacagtgacc ggtcagccag atgttgacca ggttgttctt
    23761 gatgaagcga ttaaaaaccg ctccgggatg ccggttcgtc tgaattaatc ttcaaaactt
    23821 aaagcaaaag gcggactcat aatccgcctt ttttatttgc cagaccctag ttggccggga
    23881 gtataactaa caaaacatat cccaccaacg ccgcccacaa cagcatcggt attgaataga
    23941 gatggaaacg ccaccagatg cggcgatcgt tcgccatacg tagcgcaatc aaatttgcca
    24001 gcgatccggg taataaccca aagccaccta cgtttaccgc ccataccagt aataaagacg
    24061 gcggcacata gttcagcaac aatatggtac tcggcacgtt actgatcacc tgcgataaac
    24121 cgattgccgt taaccataac ccgggttcag atagatgact cacgttaccc aacacgcctt
    24181 gcaacgctgg aagctgggtc agtaaatgga cgtcgataaa catcgccata aacaccagca
    24241 gcagcgtcca gtccacactg agcaccacgc gacgtgccag cagcgcaaag cctgccgcca
    24301 caatcaccag tccccacagc tcttgtttga actccagcgc cgtcagaaag acgatataca
    24361 gccccagaca actccacacc agccgcggtt tccactccgg tgtttgcacc cccgtatggt
    24421 attgcagcgc ctttccaggg aaacaacacc agcacaggag caggagcgtc agcatcattg
    24481 cgccagccag cggtgccatt tgggcaataa atccggcaaa cgaaagacca gaacgtcccc
    24541 agataagaat attttgcggg ttgccaattg gcgtcagtag cgaaccagcg ttgactgcca
    24601 gcgcctcaaa aataatcagc cgattaaccg ggatctcaca cagtcttttt agcgtgatag
    24661 tcagcggaac aacaataaac agcgcgacat cgttggtcag aaaggtagaa agcagcgccg
    24721 ccgccagcac cataaacatc gccagccgac gctccgtagc aaagcggcgc accattttgc
    24781 gccccagcac atcaaaataa ccgcttaact ccacaccttt ggtcagcagc atcaggccgc
    24841 ttaaggtgat gatggtgtgc cagtcgatag cagcaggcca ggatttcggt gcaaagggca
    24901 cgaaaaagct taatccgata ccaacaagaa ttaataactg aaaaaaacga tcgccttgca
    24961 gcgtgcgtaa aaaaggcagg ctcatttggc accgtgtttc tgggtaaaca aacgaaaagc
    25021 gtcgagcgtc tcttcactaa catggtgctc catgccttcc gcgtcgcgac gggcgatttc
    25081 cggactgacg cccaacacca acaagaaatt ttcgactatc tgatgtcgct cgcggctttc
    25141 ctgcgccagc ttctctcctt ctgccgttaa aaacacgcca cgccagggga tcatttcaat
    25201 cagccccatg gttgccagcc gcttaagcat tttagccacc gtcggttgcg aaactcccag
    25261 acgagcagcc atgtccacct gacgagcttc ccccacttcc ctgatcaagt cagaaatcag
    25321 ctcaacgtaa tcatcaataa gctcgcgccg atgcgcctct ctgacctggc ggaacccttc
    25381 aacgtgctct tccacgttca ctaactgcgt cacttttttt gctgttggcg tacctgcgcg
    25441 acgactcatt gtgcttcctc attatggtga cgcatacata gcgttctcat ttcgtgcgca
    25501 cattgtaaac cagagttgcg aaggtacaaa aaattaacgt tttagcaata gctacataat
    25561 atagcctgtg ctatatctgt atgtaatgca atcatccctc aaggatcgac gggattagca
    25621 agtcaggagg tcttatgaat gagttcaaga agtgtatgcg cgtgtttagt cattctccct
    25681 ttaaagtacg gttaatgctg ctctctatgt tgtgcgatat ggtcaacaac aaaccgcagc
    25741 aagataaacc ttccgataaa tagcggcgtc gcggtacgcc gcttcactcc tgcttttatg
    25801 caggcataac gcgttttggt ctgaaaaacc ccactttttg tcggatttgc aatccccttc
    25861 gcaaaagatt tgttcgtcag tagttgacct gaacggcggc tcgctctatc ttcttgcagc
    25921 cctgcgtata ttgcggctcg cggatgcgga cccctttccc ctcttcacgc actcttgcag
    25981 gtattgaccc ttgacgccag ggtaagcaca tggcgtttgt tacgatagtg gcatatgaat
    26041 ttaaccctca aagaatcgct tgttacccgt agccgggtat ttagcccgtg gactgcgttc
    26101 tactttttac agtcgctatt aattaacctc ggcttaggtt accccttcag tttgctctac
    26161 accgctgcgt ttacggctat tttgcttttg ctatggcgaa cattgcctcg cgtacaaaaa
    26221 gttctggtcg gtgtcagttc gctggtggcg gcttgttatt tcccttttgc tcaggcctac
    26281 ggcgcgccta atttcaatac attgctggca ttgcactcca ccaatatgga agagtcgacc
    26341 gaaatcctga cgatttttcc gtggtacagc tacctggtcg gcttatttat ttttgcgctc
    26401 ggcgtaatag caatcaggcg aaaaaaagag aatgaaaaag cgcgctggaa taccttcgac
    26461 agcctgtgtc tggtattcag tgtggcgaca ttttttgttg ctcccgtgca aaacctggcc
    26521 tggggtggcg tatttaaact gaaagatact ggctatccgg tatttcgttt tgctaaggat
    26581 gtcatcgtca ataataacga ggtgattgaa gagcaagaac ggatggcaaa actttccgga
    26641 atgaaagata cctggacggt cactgccgtt aagccgaagt atcagaccta tgtggtggtg
    26701 atcggcgaaa gcgcgcgtcg cgatgccctc ggtgcctttg gcggtcactg ggacaatacc
    26761 ccgtttgcca gcagcgttaa cggtttgata tttgctgact acattgccgc cagtggctcc
    26821 acgcagaaat cacttggctt aacgctcaat cgcgtagtcg atggcaaacc acagtttcag
    26881 gataactttg tcaccctggc aaatcgcgcg ggcttccaga cctggtggtt ttccaaccag
    26941 ggccaaatcg gcgaatacga tactgctatc gccagcatcg ccaaacgtgc agatgaagtg
    27001 tacttcctga aagaaggtaa ttttgaagca gataaaaaca cgaaagacga agcgttactg
    27061 gatatgaccg ctcaagtgct ggcgcaagag cactcgcaac cgcagctgat tgttctgcat
    27121 ctgatgggct cgcatccgca ggcctgcgac aggacacaag gcaaatacga aacctttgtg
    27181 caatcgaaag aaacgtcgtg ctatctctat accatgacgc aaacggacga tttactgcgc
    27241 aagctgtacg atcagttacg caacagcggc agcagcttct ctctggttta cttttctgac
    27301 cacggtctgg cctttaaaga gcgcggcaaa gacgtgcaat accttgccca tgatgataaa
    27361 tatcagcaaa atttccaggt gccttttatg gtcatttcca gcgacgataa agcgcatcgg
    27421 gtgattaaag cccgccgctc agccaatgac ttcttaggct ttttctcgca gtggacggga
    27481 attaaagcga aggaaataaa tatcaaatac ccgtttatat ctgagaagaa agccgggccg
    27541 atatacatca ccaacttcca gttacagaag gtggattaca accatctcgg aaccgatatt
    27601 ttcgacccga aaccttaaaa caaaaaatcc gccccgagag gcggattttt tatatcacca
    27661 aagtgattag aagcggtaac caacaccggc aatccaggtg cctacgtcaa cgctacgaat
    27721 acggctctgc tcgtaagaga agtccagagc aacgttttcc atcgggttga actgcagacc
    27781 agcaccgtag gagaaaccgt agtcgctggt gtcgtgtttg taggtcgggt attcagtggt
    27841 ctggaattta ccataaccca cccccactac accgtagatg cttgcccagt cgttaatgcg
    27901 gtaagccgga ccagcagtga tgccgtagta ctggtttttg ttgtagtcac cagagcttgc
    27961 agtacggctt ttctcggtgt aagtgaaaga accgatcaca cccagcgggc tgttgtcttc
    28021 ttcatagcgg tatttcaggt tgaaaccgcc cattttgttc atttggccct gagcgtcgct
    28081 ctgtgcgtaa ccgccagtta cggtagaagt cgcagctacg gaagtacctg cggtgaaagc
    28141 cagaactgcg gccagtgctg aaagacatgc aatttttttc ataaccacct caaatgtgat
    28201 tcaaataagt cctaagtttt aaatatatca aaaattaatg ggaaactctt cgcgatttgt
    28261 gatgtctaac gggccatttc atgtaacaga acgtttccat acaccgctat ccatctaaat
    28321 ttaaatcact ttttcagaga actgcgtaag tattacgcat gttttccctg tcattcatcc
    28381 agattattcc taatcaccag actaatgatt ccatcaatcc tggcgcattt tagtcaaaac
    28441 gggggaaaat tttttcaaca aatgctcaac cagcattggg tatatccagt acactccacg
    28501 ctttacttaa gtctagatat ttgtgggaga aaggatgcct ggttcattac gtaaaatgcc
    28561 ggtctggtta ccaatagtca tattgctcgt tgccatggcg tctattcagg gtggagcctc
    28621 gttagctaag tcactttttc ctctggtggg cgcaccgggt gtcactgcgc tgcgtctggc
    28681 attaggaacg ctgatcctca tcgcgttctt taagccatgg cgactgcgct ttgccaaaga
    28741 gcaacggtta ccgctgttgt tttacggcgt ttcgctgggt gggatgaatt atctttttta
    28801 tctttctatt cagacagtac cgctgggtat tgcggtggcg ctggagttca ccggaccact
    28861 ggcggtggcg ctgttctctt ctcgtcgccc ggtagatttc gtctgggttg tgctggcggt
    28921 tcttggtctg tggttcctgc taccgctggg gcaagacgtt tcccatgtcg atttaaccgg
    28981 ctgtgcgctg gcactggggg ccggggcttg ttgggctatt tacattttaa gtgggcaacg
    29041 cgcaggagcg gaacatggcc ctgcgacggt ggcaattggt tcgttgattg cagcgttaat
    29101 tttcgtgcca attggagcgc ttcaggctgg tgaagcactc tggcactggt cggttattcc
    29161 attgggtctg gctgtcgcta ttctctcgac cgctctgcct tattcgctgg aaatgattgc
    29221 cctcacccgt ttgccaacac ggacatttgg tacgctgatg agcatggaac cggcgctggc
    29281 tgccgtttcc gggatgattt tcctcggaga aacactgaca cccatacagc tgctggcgct
    29341 cggcgctatc atcgccgctt caatggggtc tacgctgaca gtacgcaaag agagcaaaat
    29401 aaaagaatta gacattaatt aaatttacat ttctgcatgg ttatgcataa ccatgcagaa
    29461 tttctcgcta cttttcctct acaccgtctt tatatatcga attatgcaaa atatatcgaa
    29521 ttatgcaaaa gcatatttat tccgaaaatt cctggcgagc agataaataa gaattgttct
    29581 tatcaatata tctaactcat tgaatcttta ttagttttgt ttttcacgct tgttaccact
    29641 attagtgtga taggaacagc cagaatagcg gaacacatag ccggtgctat acttagtctc
    29701 gttaattact gggacataac atcaagagga tatgaaatta tgagtaccgc taaattagtt
    29761 aaatcaaaag cgaccaatct gctttatacc cgcaacgatg tctccgacag cgagaaaaaa
    29821 gcaacagtag agttgctgaa tcgccaggtt atccagttta ttgatctttc tttgattacc
    29881 aaacaagcgc actggaacat gcgcggcgct aacttcattg ccgtacatga aatgctggat
    29941 ggcttccgca ccgcactgat cgatcatctg gataccatgg cagaacgtgc agtgcagctg
    30001 ggcggtgtag ctctggggac cactcaagtt atcaacagca aaaccccgct gaaaagttac
    30061 ccgctggaca tccacaacgt tcaggatcac ctgaaagaac tggctgaccg ttacgcaatc
    30121 gtcgctaatg acgtacgcaa agcgattggc gaagcgaaag atgacgacac cgcagatatc
    30181 ctgaccgccg cgtctcgcga cctggataaa ttcctgtggt ttatcgagtc taacatcgaa
    30241 taaatccatc gctgatggtg cagaacttta gtacccgata aaagcggctt cctgacagga
    30301 ggccgttttg ttttgcagcc cacctcaacg cacttattta gtgcatccat ctgctatctc
    30361 cagctgatta agtaaatttt ttgtatccac atcatcacac aatcgttaca taaagattgt
    30421 tttttcatca ggttttacgc taaataatca ctgtgttgag tgcacaattt tagcgcacca
    30481 gattggtgcc ccagaatggt gcatcttcag ggtattgccc tataaatcgt gcatcacgtt
    30541 tttgccgcat ctcgaaaaat caaggagttg caaaactggc acgatttttt catatatgtg
    30601 aatgtcacgc aggggatcgt cccgtggata gaaaaaagga aatgctatga agtctgtatt
    30661 aaaagtttca ctggctgcac tgaccctggc ttttgcggtt tcttctcatg ccgcggataa
    30721 aaaattagtt gtcgcgacgg ataccgcctt cgttccgttt gaatttaaac agggcgataa
    30781 atatgtgggc tttgacgttg atctgtgggc tgccatcgct aaagagctga agctggatta
    30841 cgaactgaag ccgatggatt tcagtgggat cattccggca ctgcaaacca aaaacgtcga
    30901 tctggcgctg gcgggcatta ccatcaccga cgagcgtaaa aaagcgatcg atttctctga
    30961 cggctactac aaaagcggcc tgttagtgat ggtgaaagct aacaataacg atgtgaaaag
    31021 cgtgaaagat ctcgacggga aagtggttgc tgttaagagc ggtactggct ccgttgatta
    31081 cgcgaaagca aacatcaaaa ctaaagatct gcgtcagttc ccgaacatcg ataacgccta
    31141 tatggaactg ggcaccaacc gcgcagacgc cgttctgcac gatacgccaa acattctgta
    31201 cttcatcaaa accgccggta acggtcagtt caaagcggta ggtgactctc tggaagcgca
    31261 gcaatacggt attgcgttcc cgaaaggtag cgacgagctg cgtgacaaag tcaacggcgc
    31321 gttgaaaacc ctgcgcgaga acggaactta caacgaaatc tacaaaaaat ggttcggtac
    31381 tgaaccgaaa taataacgct acacctgtaa agcgcactgg cagttccctc tcccctctgg
    31441 ggagaggatt agggtgaggg gcgcaaaccc gctccggggc cattaattac cctgaatttg
    31501 attatttaca ccacggtaac aggaacaaca tatgcagttt gactggagtg ccatctggcc
    31561 tgccattccg cttctgattg aaggtgccaa aatgaccctg tggatttcgg tcctcggtct
    31621 ggctggcggt ctggtaatcg gattgctggc aggttttgca cgcaccttcg gtggttggat
    31681 agccaaccac gtcgcgctgg tctttattga ggtgatccgc ggcacaccta tcgtcgtcca
    31741 ggtgatgttt atttatttcg ccctgccgat ggcgtttaac gacttacgca tcgacccatt
    31801 tactgcggcg gtggtcacca tcatgatcaa ctccggcgcg tacattgcgg aaatcacgcg
    31861 tggtgcggtg ctgtctattc acaaaggttt tcgtgaagca ggactggcgc tcggtctttc
    31921 acgttgggaa accattcgct acgtcatttt accgctggca ctgcgtcgta tgctgccgcc
    31981 gctgggtaac cagtggatca tcagcattaa agacacctcg ctgtttattg tgatcggcgt
    32041 ggcggaactg acccgtcagg ggcaagaaat tattgccggt aacttccgcg cccttgagat
    32101 ctggagcgcc gtggcggtgt tctatctgat tattaccctg gtgctaagct ttattttgcg
    32161 tcgtctggaa agaaggatga aaatcctgtg attgaattta aaaacgtctc caagcacttt
    32221 ggcccaaccc aggtgcttca caatatcgat ttgaacattg cccaaggcga agtcgtggtg
    32281 attatcgggc cgtccggttc cggtaaatcg accctgctgc gctgcatcaa caaactggaa
    32341 gaaatcacct ccggcgatct gattgtcgat ggcctgaagg ttaacgatcc gaaagttgac
    32401 gagcgcctga ttcgccagga agcaggtatg gtgttccagc agttttacct cttcccgcat
    32461 ctgacggcgc tggaaaacgt catgtttggt ccgctacgcg tgcgtggcgc gaacaaagaa
    32521 gaggcggaaa aactggcgcg tgagctgctg gcgaaagtcg gtctggcaga acgtgcacat
    32581 cactaccctt ccgaactttc tggtggtcaa cagcagcgtg tggcgattgc ccgcgcgctg
    32641 gcggtgaagc cgaaaatgat gctgtttgat gaaccgactt ccgctcttga cccggaactg
    32701 cgccatgaag tgctgaaggt tatgcaggat ctggctgaag aagggatgac gatggtgatc
    32761 gtgacccacg aaatcggttt tgccgagaaa gtagcttcgc ggctgatctt tatcgacaaa
    32821 ggccggattg cggaagatgg cgatccgcag gtattgatca agaacccgcc gagccagcgc
    32881 ttgcaggaat ttttgcagca cgtctcttaa cttctctgcc tccctttcac ccgaaaggga
    32941 ggcacaccag attcctctca tttaaaatcg cccctccccc agcatctata cttatctttt
    33001 tgctctgttt tctcactgga ggagtcatgc ggtggatcct gttcatcctc ttctgcctgc
    33061 tgggcgcacc tgcccacgcg gtatccatac ccggcgttac aaccacaacg acaacggact
    33121 caacgactga accggccccg gaaccggata tcgaacaaaa aaaagcggcc tatggcgcac
    33181 tggcggatgt gctggataac gacacctcgc gtaaagaatt gatcgaccag ttgcgtactg
    33241 tcgccgcgac gccccctgcg gaaccggtcc cgaagatcgt gccgccgacg ctggttgaag
    33301 agcaaaccgt gctgcaaaag gtcaccgaag tcagccgcca ttatggtgaa gccctttccg
    33361 cccgcttcgg gcaactttat cgcaatatca ccggctcccc gcataagcca tttaatccac
    33421 aaaccttcag caatgcgctg agccattttt caatgttagc ggtattagtg tttggttttt
    33481 actggctgat tcgcctgtgc gcactgccgc tgtatcgcaa aatgggtcag tgggcgaggc
    33541 aaaaaaatcg tgagcgcagc aactggttgc agcttccggc gatgattatc ggggcgttta
    33601 ttatcgacct gctgttactg gccctgacat tatttgtcgg ccaggtatta agcgacaacc
    33661 tgaatgcagg cagtcgcacc atcgctttcc aacaaagttt gtttctcaac gcctttgccc
    33721 tgattgaatt tttcaaagcc gtactacgcc tgattttttg cccaaacgtg gcggagctgc
    33781 gcccgttcac gattcaggac gagagcgccc gttactggag tcgtcgcctg agctggttaa
    33841 gcagtttgat aggttatggc ctgattgtgg ccgtgccgat tatctctaat caggtgaatg
    33901 tacagatagg tgcgctggcg aacgtcatca ttatgctgtg catgaccgtc tgggcgttgt
    33961 acctgatctt tcgtaataaa aaagagatta cccagcattt gctcaacgtt gcggagcatt
    34021 cgctggcctt tttcagcctg tttatccgcg cctttgcgct ggtgtggcac tggctggcaa
    34081 gcgcctattt tatcgtgctg tttttctttt cgttgttcga tccgggcaac agcctgaaat
    34141 ttatgatggg tgcaacggtg cgcagcctgg cgattattgg tatcgcagcg tttgtttccg
    34201 gtatgttttc ccgctggctg gcaaaaacca tcactctctc gccacatact cagcgtaact
    34261 atccagagtt gcaaaaacgg ctgaatggct ggctttcggc ggcgctgaaa acggcgcgta
    34321 ttctgaccgt ctgcgtggcg gtaatgttgc tgttgagcgc atggggattg ttcgatttct
    34381 ggaactggct gcaaaacggc gcggggcaga aaaccgtaga tatcctgatc cgtatcgcac
    34441 ttattctttt tttctcggcg gttggctgga cggtgctcgc cagtttgatc gaaaaccggc
    34501 tggcttcgga tattcatggc cgcccgctac ccagcgcccg tacgcgtacc ctgctgacgc
    34561 tgttccgtaa cgcgctggcg gtgattatca gtaccatcac catcatgatt gtgttgtcgg
    34621 aaatcggcgt caatatcgcg ccattgctgg caggtgccgg ggcattaggt ctggctatct
    34681 cgtttggttc gcaaacgctg gtgaaagata tcattaccgg ggtgtttatt cagtttgaaa
    34741 acggcatgaa cactggagat ttggtgacta tcgggccgtt gaccggcaca gtggaacgga
    34801 tgtcgattcg ctccgtgggc gtgcgccagg ataccggggc gtatcacatc attccgtggt
    34861 cttcgataac cacctttgct aacttcgtcc gcggtattgg ttcggtagtg gcaaactacg
    34921 atgttgatcg ccatgaagat gctgataaag ccaatcaggc actgaaagat gcggtagcgg
    34981 aattaatgga aaacgaagaa attcgcgggc tgattattgg tgaaccgaat tttgccggga
    35041 ttgtcggctt aagcaatacc gcgtttacac tgcgtgtttc gttcaccacg ctgccactca
    35101 aacagtggac ggtacgcttt gccctcgaca gccaggtgaa aaaacatttc gacctggcgg
    35161 gcgttcgcgc gccagtgcag acttatcagg tgctgcctac tccgggcgcg accccggctg
    35221 aaccgttacc gccgggggag ccaacgcttt aacgctggcg attgacaaaa cggcggcgct
    35281 gctcgtcgtt cataaaggtc caggcaataa agcgactctg cttttgcccc tgggccatct
    35341 ctttttttac caccttcacc gcgcccacgt cggtcagagc acgatacaac ggcggtaagt
    35401 tttcaccacg agataccagc gaggtaaacc acatcacctg cttcgcgaag cctttgctct
    35461 cttcaatcat ctttttgata aaggcgactt caccaccttc acaccacaac tcctgttgct
    35521 gaccaccaaa gttcagtgca tcgtctttgt tcagccccag gttacggcgt ttacgctcac
    35581 tacctgcccg cgctgcagcg gcggaatcgt ggaatggcgg gttacacaag gtcgcgtcgt
    35641 attgctcgtt tttatggatg atgccgttaa aaattgcccc actctctttt tgccgacgca
    35701 gacgaatggc gcggttaagc cccggattag cactgataat cgcctgcgca ctgcttaacg
    35761 cctggctgct ggtttcacta ccggtaaaac gccagccata ttcatgtacg ccaatcagcg
    35821 gataaataca gttcgcacca acgccgatat ccagaatgct ggcattcgct ggaattgttc
    35881 cgcttgcttc agccagtaaa tcggcaaggt gatgaatata atccgcccgg cccggtactg
    35941 gtgggcagag aaaaccgtcg gggatatccc agttcgctac ggcgtaaaaa tgggccagca
    36001 acgccttatt gagcgccttc accgccagcg gattggcaaa gtctacgctt tgctccccgg
    36061 cgggtgtaag cgtgaggaat tgcctgagtt caggattgac ctgacaaagc gtggcgagat
    36121 cgtagcggct gtgatgacgg ttgcgcggat gcaaccccgg tttctgggcg gacatagcat
    36181 tctcctttaa gcagcggcgt aagatacccg cataacgcga cagggtaaat atctggctgc
    36241 gtttatcatt ccccaaaacc ttaacatggc tatggtttat ggcaattcat cataaaaatc
    36301 agcattatgc atgtcattaa tttgtcttat tttgtaaaaa tatatcgttc aaaaaataag
    36361 cacatctctg tcaatttttt gcgtcatttg catcgcgcag gcgacctaca cttatcaggc
    36421 actacccgtt gtatttatga ggatgtcatt atgaaaaagt gcctcacact actgattgcc
    36481 accgtcctga gcggaatctc tctcacggct tatgccgcgc aaccgatgag taacctggac
    36541 agcggtcagc tacggcccgc cggtaccgtt tcggcaactg gcgcatcaaa cctaagcgat
    36601 ctggaggata aactggcaga aaaagcgcgc gaacaaggcg cgaaaggtta tgtcattaat
    36661 tccgcaggcg gaaatgacca gatgttcggt actgcaacca tctacaaata accccaccaa
    36721 cgtaactgca ttcgcctctg gatgcagttt tcgcctgttt tgtaatcaaa aaacatcaaa
    36781 tatgaactca atgtaaataa atgtatttct ttttcgcgca atgggtgata gaaaatcgcg
    36841 ccaagtgata atgcttatca aaattattat cacttgcacg aacactatca cgggattaac
    36901 agtggcatcg catccgcaga gatgctttct cgtggcagtg aaaatttcaa catataagaa
    36961 aaagtcacct gcaaaatgga aaacaatcgc aatttccctg ccagacaatt tcattcgctc
    37021 acgttctttg ccggtctttg tattggcatc acgcctgtgg ctcaggcact cgccgccgaa
    37081 gggcaaacta acgcggatga cacgctggtt gtcgaagcat caacgccttc gctttatgcg
    37141 ccacaacaat ctgccgatcc gaaattctcg cgtccggtag cggatactac ccgcacgatg
    37201 acggtgattt ctgaacaagt gattaaagat cagggcgcaa ccaaccttac cgacgcgctc
    37261 aaaaacgccc ccggcg
//
LOCUS       DAKISA010000036        35537 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.036, whole genome shotgun sequence.
ACCESSION   DAKISA010000036 DAKISA010000000
VERSION     DAKISA010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35537
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.036"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            118..882
                     /gene="ycaL"
                     /locus_tag="OVW45_001847"
     CDS             118..882
                     /gene="ycaL"
                     /locus_tag="OVW45_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCV5526314.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            1055..1738
                     /gene="cmk"
                     /locus_tag="OVW45_001848"
     CDS             1055..1738
                     /gene="cmk"
                     /locus_tag="OVW45_001848"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCV5526315.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            1849..3522
                     /gene="rpsA"
                     /locus_tag="OVW45_001849"
     CDS             1849..3522
                     /gene="rpsA"
                     /locus_tag="OVW45_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCV5526316.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            3682..3966
                     /gene="ihfB"
                     /locus_tag="OVW45_001850"
     CDS             3682..3966
                     /gene="ihfB"
                     /locus_tag="OVW45_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCV5526317.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            4173..6437
                     /locus_tag="OVW45_001851"
     CDS             4173..6437
                     /locus_tag="OVW45_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HCV5526318.1"
                     /translation="MKITTVDVCIICGIFPLLILPQLPGTLALAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAVLLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQITVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            6474..8222
                     /gene="msbA"
                     /locus_tag="OVW45_001852"
     CDS             6474..8222
                     /gene="msbA"
                     /locus_tag="OVW45_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCV5526319.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAIDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            8219..9205
                     /gene="lpxK"
                     /locus_tag="OVW45_001853"
     CDS             8219..9205
                     /gene="lpxK"
                     /locus_tag="OVW45_001853"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCV5526320.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            9242..10474
                     /locus_tag="OVW45_001854"
     CDS             9242..10474
                     /locus_tag="OVW45_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCV5526321.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            10526..10708
                     /gene="ycaR"
                     /locus_tag="OVW45_001855"
     CDS             10526..10708
                     /gene="ycaR"
                     /locus_tag="OVW45_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCV5526322.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            10705..11451
                     /gene="kdsB"
                     /locus_tag="OVW45_001856"
     CDS             10705..11451
                     /gene="kdsB"
                     /locus_tag="OVW45_001856"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCV5526323.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            11605..12498
                     /locus_tag="OVW45_001857"
     CDS             11605..12498
                     /locus_tag="OVW45_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCV5526324.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(12475..13254)
                     /gene="elyC"
                     /locus_tag="OVW45_001858"
     CDS             complement(12475..13254)
                     /gene="elyC"
                     /locus_tag="OVW45_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCV5526325.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEELNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPWQE"
     gene            13390..14175
                     /gene="cmoM"
                     /locus_tag="OVW45_001859"
     CDS             13390..14175
                     /gene="cmoM"
                     /locus_tag="OVW45_001859"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCV5526326.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            14172..15494
                     /gene="mukF"
                     /locus_tag="OVW45_001860"
     CDS             14172..15494
                     /gene="mukF"
                     /locus_tag="OVW45_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCV5526327.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            15475..16179
                     /gene="mukE"
                     /locus_tag="OVW45_001861"
     CDS             15475..16179
                     /gene="mukE"
                     /locus_tag="OVW45_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCV5526328.1"
                     /translation="MSSTNIEQVMSVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            16179..20639
                     /gene="mukB"
                     /locus_tag="OVW45_001862"
     CDS             16179..20639
                     /gene="mukB"
                     /locus_tag="OVW45_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCV5526329.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTAQRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNVVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            20900..22747
                     /gene="ldtD"
                     /locus_tag="OVW45_001863"
     CDS             20900..22747
                     /gene="ldtD"
                     /locus_tag="OVW45_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCV5526330.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAVFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            22928..23476
                     /locus_tag="OVW45_001864"
     CDS             22928..23476
                     /locus_tag="OVW45_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCV5526331.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            23503..24150
                     /gene="gloC"
                     /locus_tag="OVW45_001865"
     CDS             23503..24150
                     /gene="gloC"
                     /locus_tag="OVW45_001865"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCV5526332.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(24372..25562)
                     /gene="aspC"
                     /locus_tag="OVW45_001866"
     CDS             complement(24372..25562)
                     /gene="aspC"
                     /locus_tag="OVW45_001866"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCV5526333.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(25747..26835)
                     /gene="ompF"
                     /locus_tag="OVW45_001867"
     CDS             complement(25747..26835)
                     /gene="ompF"
                     /locus_tag="OVW45_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCV5526334.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(27437..28837)
                     /gene="asnS"
                     /locus_tag="OVW45_001868"
     CDS             complement(27437..28837)
                     /gene="asnS"
                     /locus_tag="OVW45_001868"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCV5526335.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(29007..30209)
                     /gene="pncB"
                     /locus_tag="OVW45_001869"
     CDS             complement(29007..30209)
                     /gene="pncB"
                     /locus_tag="OVW45_001869"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCV5526336.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            30475..33087
                     /gene="pepN"
                     /locus_tag="OVW45_001870"
     CDS             30475..33087
                     /gene="pepN"
                     /locus_tag="OVW45_001870"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCV5526337.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(33294..34054)
                     /gene="ssuB"
                     /locus_tag="OVW45_001871"
                     /pseudo
     CDS             complement(33294..34054)
                     /gene="ssuB"
                     /locus_tag="OVW45_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
     gene            complement(34051..34842)
                     /gene="ssuC"
                     /locus_tag="OVW45_001872"
     CDS             complement(34051..34842)
                     /gene="ssuC"
                     /locus_tag="OVW45_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HCV5526338.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(34854..>35537)
                     /locus_tag="OVW45_001873"
     CDS             complement(34854..>35537)
                     /locus_tag="OVW45_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCV5526339.1"
                     /translation="IHVRGAKLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWG
                     EPPELVKEKIEQVRAKAAAHGRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIA
                     KAQAAFARTDSVGQQRMAALHNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAA
                     RINEYAALGIDSFVLSGYPHLEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVAND
                     FIPRKVAQS"
BASE COUNT         8685 a         8892 c         9491 g         8469 t
ORIGIN      
        1 cattgtcggc gttttttttc ggaccttgtg agtcattttg attaatggta gcgtcgcttg
       61 tcaatgtaag ttgttgatac ataatattta tatatgatta atcaacggat gattcacatg
      121 aagaatacta aattactgct ggcgattgcg acctctgcag cattactgac agggtgtcaa
      181 aatacccacg gtattgatac caatatggct atcagctccg gtttaaatgc ctataaagca
      241 gcaacattaa gcgatgccga tgcaaaagcg attgccaatc agggctgtgc cgaaatggac
      301 agcggcaatc aagtcgcaag taaatccagc aagtacggta aacgtctggc aaaaatcgcc
      361 aaagcattgg gtaacaatat taatggcacg ccggtcaact ataaggttta tatgaccagc
      421 gacgtcaacg catgggcgat ggcgaacggc tgtgtccgtg tctacagtgg cctgatggac
      481 atgatgaatg acaacgaaat tgaaggtgtt ctgggccatg aactgggcca cgtcgcgttg
      541 ggtcactcgc tggctgaaat gaaagcttct tatgcgatcg ttgccgcacg cgatgccatt
      601 tcagctacca gcggtgtggc ttcccagctt tcccgctcac aattgggtga tatcgcagaa
      661 ggcgctatta atgctaaata ctcccgtgat aaagagtccg aagcagatga tttctccttt
      721 gatctgttga agaaacgtgg catcagcacc caggggctgg ttggcagctt tgaaaaactg
      781 gctagtctgg atggcggtcg cacccagtcc atgtttgact ctcacccacc atcaacagag
      841 cgtgcgcaac acatccgtga tcgtatcgcc tctggtaagt aaatcattgt catctttcgg
      901 gctggtcttc tgccagcccg ctataattgc gcaataaatc cccatctgaa tacagacaaa
      961 actggttttt gcacacaacg ttaacgattt gtggcgtcgg cgcgtataat gcgcgcggtt
     1021 atgttaacgg tacgcctgtt ttaaggagat aaagatgacg gcaattgccc cggttattac
     1081 cattgatggc ccaagcggtg cagggaaagg caccttgtgt aaggctatgg cggaagcatt
     1141 gcaatggcat ctgctggatt cgggtgcaat ttatcgcgta ctggcgttgg cggcattaca
     1201 tcaccatgtt gatgttgcgt cagaagatgc gctggtaccg ctggcatccc atctggatgt
     1261 acgttttgtg tcaaccaatg gcaatctgga agtgatcctc gaaggggaag atgtcagcgg
     1321 cgaaattcgt actcaggaag tggcgaatgc agcttcacaa gtcgcggcat tcccacgcgt
     1381 tcgtgaagca ttattgcgtc gccaacgcgc gtttcgcgaa ttaccaggcc tgattgccga
     1441 tggccgcgac atgggaacgg tggtattccc tgatgcgcca gtgaaaattt tccttgacgc
     1501 ctcctcggaa gaacgtgcgc atcgccgcat gctacagttg caggagaagg gctttagtgt
     1561 taactttgag cgccttttgg ccgagatcaa agaacgcgac gaccgcgatc gtaaccgagc
     1621 ggtagcgcca ctggttccgg cagccgatgc tttagtgttg gattccacca ccttaagcat
     1681 tgagcaagtg attgaaaaag cgctacaata cgcgcgccag aaattggctc tcgcataagc
     1741 gaccgaattt gcagtacccc cgttgcaatg gaatgacagc gggtatgtta aacaacccca
     1801 tccggcatgg agccaggtgg acgttaaata taaacctgaa gattaaacat gactgaatct
     1861 tttgctcaac tctttgaaga gtccttaaaa gaaatcgaaa cccgcccggg ttctatcgtt
     1921 cgtggcgttg ttgttgctat cgacaaagac gtagtactgg ttgacgctgg tctgaaatct
     1981 gagtccgcca tcccggctga gcagttcaaa aacgcccagg gcgagctgga aatccaggta
     2041 ggtgacgaag ttgacgttgc tctggacgca gtagaagacg gcttcggtga aactctgctg
     2101 tcccgtgaga aagctaaacg tcacgaagcc tggatcacgc tggaaaaagc ttacgaagat
     2161 gctgaaactg ttaccggtgt tatcaacggc aaagttaagg gcggcttcac tgttgagctg
     2221 aacggtattc gtgcgttcct gccaggttct ctggtagacg ttcgtccggt gcgtgacact
     2281 ctgcacctgg aaggcaaaga gcttgaattt aaagtaatca agctggatca gaagcgcaac
     2341 aacgttgttg tttctcgtcg tgccgttatc gaatccgaaa acagcgcaga gcgcgatcag
     2401 ctgctggaaa acctgcagga aggcatggaa gttaaaggta tcgttaagaa cctcactgac
     2461 tacggtgcat tcgttgatct gggcggcgtt gacggcctgc tgcacatcac tgacatggcc
     2521 tggaaacgcg ttaagcatcc gagcgaaatc gtcaacgtgg gcgacgaaat cactgttaaa
     2581 gtgctgaagt tcgaccgcga acgtacccgt gtatccctgg gcctgaaaca gctgggcgaa
     2641 gatccgtggg tagctatcgc taaacgttat ccggaaggta ccaaactgac tggtcgcgtg
     2701 accaacctga ctgactacgg ctgcttcgtt gaaatcgaag aaggcgttga aggtctggta
     2761 cacgtttccg aaatggattg gaccaacaaa aacatccacc cgtccaaagt tgttaacgtt
     2821 ggcgatgtag tggaagttat ggttctggat atcgacgaag aacgtcgtcg tatctccctg
     2881 ggtctgaaac agtgcaaagc taacccgtgg cagcagttcg cggaaaccca caacaagggc
     2941 gaccgtgttg aaggtaaaat caagtctatc actgacttcg gtatcttcat cggcctggac
     3001 ggcggcatcg acggcctggt tcacctgtct gacatctcct ggaacgttgc aggcgaagaa
     3061 gcagttcgtg aatacaaaaa aggcgacgaa atcgctgcag ttgttctgca ggttgacgca
     3121 gaacgtgaac gtatctccct gggcgttaaa cagctcgcag aagatccgtt caacaactgg
     3181 gttgctctga acaagaaagg cgctatcgta accggtaaag taactgcagt tgacgctaaa
     3241 ggcgcaaccg tagaactggc tgacggcgtt gaaggttacc tgcgtgcttc tgaagcatct
     3301 cgtgaccgcg ttgaagacgc taccttggtt ctgagcgttg gcgacgaagt tgaagctaaa
     3361 ttcaccggcg ttgatcgtaa aaaccgcgca atcagcctgt ctgttcgtgc gaaagacgaa
     3421 gctgacgaga aagatgcaat cgcaactgtt aacaaacagg aagatgcaaa cttctccaac
     3481 aacgcaatgg ctgaagcttt caaagcagct aaaggcgagt aattctctga cttttcggga
     3541 tttttattcc gaagtttgtt gagtttactt gacagattgc aggtttcgtc ctgtaatcaa
     3601 gcactaaggg cggctacggc cgcccttaat caatgcagca acagcagccg cttaatttgc
     3661 ctttaaggaa ccggaggaat catgaccaag tcagaattga tagaaagact tgccacccag
     3721 caatcgcaca ttcccgccaa gacggttgaa gatgcagtaa aagagatgct ggagcatatg
     3781 gcctcgactc ttgcgcaggg cgagcgtatt gaaatccgcg gtttcggcag tttctctttg
     3841 cactaccgcg caccacgtac cggacgtaat ccgaagactg gcgataaagt agaactggaa
     3901 ggaaaatacg ttcctcactt taaacctggt aaagaactgc gcgatcgcgc caatatttac
     3961 ggttaagttt ttactcaaac ttgaacgcga gaaaagcacc tgtcgggtgc ttttttcatt
     4021 tctctaatca ggaactggaa gctgcctcgc agagttttga gcagttttca ccctctcgtt
     4081 aaattcttct gaatgtgcct cggggaacgc aaaattccca ccagacaacc gctcaacaaa
     4141 gttgcacact ttccataaac agggaggagt gcatgaaaat aacgacagtc gacgtatgca
     4201 taatttgcgg aatttttccg ttgctgattt tgccccaatt gcctgggaca ttagcccttg
     4261 cgtttctgac tctcttcgcc tgcgtactgg catttatacc tgttaaaacc gtccgttata
     4321 tcgcgctgac gttgctgttt ttcgtttggg gcatattatc agcaaagcaa attttgtggg
     4381 caggagaaac cttaactggc gcgacgcagg atgcaattgt tgagatcact gctactgacg
     4441 gcatgaccac tcattacggt caaattactt atctacaagg tcgacgtata ttccctgcgc
     4501 caggccttgt gctgtatggc gaatatcttc cgcaagcggt ttgtgccgga caactgtggt
     4561 caatgaaact caaagttcgt gccgttcatg gtcaacttaa tgatggcggc tttgatagcc
     4621 agcgttatgc cattgcccag catcagccgc tcaccggccg ctttctgcag gcaagtgtta
     4681 ttgaaccgaa ttgtagcctg cgtgcacagt atctggcgtc actacaaaca acgctgcaac
     4741 cctatccgtg gaatgcggtt attcttggtt taggtatggg ggaacggtta tccgtcccca
     4801 aagaaatcaa aaatatcatg cgcgatactg gaacggcgca tttaatggcg atatcgggat
     4861 tgcacatcgc ttttgcggtg ttgctggctg ccggactcat tcgcagtgga caaatttttc
     4921 tgcctgggcg ctggatccac tggcaaatac cattaattgg tgggatctgc tgtgctgctt
     4981 tttatgcctg gttgacggga atgcaacctc ctgcattgcg taccgtggtg gcgcttgcta
     5041 cgtggggaat gcttaagtta agtgggcgac agtggagtgg ctgggatgta tggatatgtt
     5101 gtctggcggc aattttgctg atggatcctg ttgccattct ctcgcaaagt ttatggctct
     5161 ctgccgctgc ggtcgcggca ctgatttttt ggtatcagtg gtttccctgt cctgagtggc
     5221 aactgccgcc ggtattgcgt gcagttgttt ccctcatcca tctgcaactg ggtatcacac
     5281 ttctgcttat gcccgtgcaa atcgtcatat ttcatggcat tagtctgacc tcgtttattg
     5341 caaatctatt agcaattccc ttggtgacat ttatcacggt tccgttgatc ctcgccgcga
     5401 tggttgtgca tttaagcggg ccgttaatcc tggagcaagg gttatggttt cttgccgacc
     5461 ggtctttggc tttacttttc tgggggttaa agagtttgcc agaagggtgg atcaacattg
     5521 ctgaacgttg gcaatggcta tcattttccc catggttctt actggtggta tggcgattaa
     5581 acgcctggcg aacgttgcca gcaatgtgtg tggctgtagg cttgctgatg tgctggccgc
     5641 tgtggcaaaa acctcgacct gacgagtggc aggtgtacat gcttgatgtc gggcaagggc
     5701 tggcaatggt gatagccaga aacggcaaag cgattctcta tgacacagga ctggcctggc
     5761 ccgaagggga tagtgggcaa caactgatta tcccctggct ccactggcat aatcttgaac
     5821 cggaaggcgt tattctgagt catgaacatc tggatcaccg gggagggctg gactcaatat
     5881 tgcacacatg gccgatgtta tggatcagaa gtccgttaaa ctgggaacat catcagccct
     5941 gtgtgcgtgg cgaagcgtgg caatggcaag gattgcgttt cagcgtgcac tggcctttac
     6001 aagctagcaa cgataaagga aataaccatt cctgtgtggt taaggttgat gacgggacga
     6061 atagcattct tctaaccggt gatattgaag tccccgctga acaaaagatg ctaagccgtt
     6121 actggcagca agtgcagaca acattgcttc aggtacctca ccatggcagt aatacctcat
     6181 catcgttgcc attaattcag cgagtgaatg gaaaagtggc actcgcatcg gcatcgcgct
     6241 ataacgcatg gcgattgccc tcaagcaaag ttaaacatcg ctatcaacaa cagggttata
     6301 agtggcttga tactcctcat caggggcaaa taacggtcaa tttttcagcg caaggctggc
     6361 ggattagcag cctcagggag caaattttac ctcgttggta tcatcagtgg tttggcgtgc
     6421 cagtggataa cgggtagaat atgcggctat ttcaacaaat gctggttttt tgaatgcata
     6481 acgacaaaga tctctctacg tggcagacgt tccgccgact gtggccaacc attgcgcctt
     6541 ttaaagcggg tctgatcgtg gcgggcgtag cgttaatcct caacgcagcc agcgatacct
     6601 tcatgttatc gctccttaag ccacttcttg atgatggctt tggtaaaaca gatcgctccg
     6661 tgctggtgtg gatgccgctg gtggtgatcg ggctgatgat tttacgtggt atcaccagct
     6721 atgtctccag ctactgtatc tcctgggtat caggaaaggt ggtaatgacc atgcgtcgcc
     6781 gcctgtttgg tcacatgatg ggaatgccgg tttcattttt tgacaaacag tcaacgggta
     6841 cgctgttgtc acgtatcacc tacgattccg aacaggttgc ttcttcttct tccggcgcac
     6901 tgataacggt tgtgcgtgaa ggtgcgtcaa tcatcggcct gttcatcatg atgttctatt
     6961 acagttggca actgtcgatc attttgattg tgctggcacc gattgtttcg attgcgattc
     7021 gcgttgtatc gaagcgtttt cgcaacatca gtaaaaacat gcagaacacc atggggcagg
     7081 tgaccaccag cgcagaacaa atgctgaagg gccacaaaga agtattgatt ttcggtggtc
     7141 aggaagtgga aacgaaacgc ttcgataaag tcagtaaccg aatgcgtctt caggggatga
     7201 aaatggtttc agcctcttcc atctctgatc cgatcattca gctaatcgcc tctttggcgc
     7261 tggcgtttgt tctgtatgcg gcgagcttcc caagtgttat ggataacctg actgccggta
     7321 cgattaccgt tgttttctct tcaatgattg cactgatgcg tccgctgaaa tcgctgacca
     7381 acgttaacgc ccagttccag cgcggtatgg cggcttgtca gacgctgttt accattctgg
     7441 acagtgagca ggagaaagac gaaggtaagc gcgtgatcga gcgtgcgact ggcgacgtgg
     7501 aattccgcaa tgtcaccttt acttatccgg gacgtgacgt gcccgcattg cgtaacatca
     7561 acctgaaaat tccggcaggg aaaacggtcg ctctggttgg acgctctggt tcaggtaaat
     7621 caaccatcgc cagcctgatc acgcgttttt acgatattga tgaaggcgaa atcctgatgg
     7681 atggtcacga tctgcgcgag tataccctgg cgtcgttacg taaccaggtt gctctggtgt
     7741 cgcagaatgt ccatctgttt aacgatacgg ttgctaacaa cattgcttac gcacggactg
     7801 aacagtacag ccgtgagcaa attgaagaag cggcgcgtat ggcctacgcc atagacttca
     7861 tcaataagat ggataacggt ctcgatacag tgattggtga aaacggcgtg ctgctctctg
     7921 gcggtcagcg tcagcgtatt gctatcgctc gagccttgtt gcgtgatagc ccgattctga
     7981 ttctggacga agctacctcg gcgttggata ccgaatccga acgtgcgatt caggcggcac
     8041 tggatgagtt gcagaaaaac cgtacctctc tggtgattgc ccaccgcttg tctaccattg
     8101 aaaaggcaga cgaaatcgtg gtcgttgagg atggtgtcat tgtggaacgc ggtacgcata
     8161 acgatttgct tgagcaccgc ggcgtttacg cgcaacttca caaaatgcag tttggccaat
     8221 gatcgaaaaa atctggtctg gtgaatcccc tttgtggcgg ctattgctgc cactctcctg
     8281 gttgtatggc ctggtgagtg gcgcgatccg tctttgctat aaactaaaac tgaagcgcgc
     8341 ctggcgtgcc cccgtaccgg ttgtcgtggt tggtaatctc accgcaggcg gcaacggaaa
     8401 aaccccggtc gttgtctggc tggtggaaca gttgcaacag cgcggtattc gcgtgggggt
     8461 cgtatcgcgg ggatatggtg gtaaggctga atcttatccg ctgttattgt cggcagatac
     8521 cacaacagca caggcgggtg atgaacctgt gttgatttat caacgcactg atgcgcctgt
     8581 tgcggtttct cccgttcgtt ctgatgcggt aaaagccatt ctggcgcaac accctgatgt
     8641 gcagatcatc gtaaccgacg acggtttaca gcattaccgt ctggcgcgtg atgtggaaat
     8701 tgtcgttatt gatggtgtgc gtcgctttgg caatggctgg tggttgccgg cggggccaat
     8761 gcgtgagcga gcggggcgct taaagtcggt tgatgcggta atcgtcaacg gcggtgtccc
     8821 tcgcagcggt gaaatcccca tgcatctgct gccgggtcag gcggtgaatt tacgtaccgg
     8881 tacgcgttgt gacgttgctc agcttgaaca tgtagtggcg atggcgggga ttgggcatcc
     8941 gccgcgcttt tttgccacgc tgaagatgtg tggcgtacaa ccggaaaaat gtgtaccgct
     9001 ggccgatcat cagtctttga accatgcgga tgtcagtgcg ttggtaagcg ccgggcaaac
     9061 gctggtaatg actgaaaaag atgcggtgaa atgccgggcc tttgcagaag aaaattggtg
     9121 gtatttgcct gtagacgcac agctttcagg tgatgaacca gcgaaactgc ttacgcaact
     9181 aacctcgctg gcttctggca actagttacg ccgcggcagc gttcgattga tggagtcatg
     9241 aatgtcactg ccgcacctct cccttgctga tgcgcgtaat cttcaccttg ccgcacaagg
     9301 cctgttaaac aaaccccgcc gtcgagcgtc gttggaggat attccggcaa cgatctcccg
     9361 catgtccttg ctgcaaatcg ataccatcaa tattgttgcc cgtagtccat atctggtgct
     9421 tttcagtcgt ctgggaaatt atcctgccca gtggctggat gagtctctgg cgcgtggcga
     9481 attaatggaa tactgggcgc atgaagcctg ctttatgccg cgtagcgact ttcgtcttat
     9541 tcgccaccgc atgctggcac ctgaaaaaat gggctggaaa tacaaagacg cctggatgca
     9601 ggaacatgag gcggaaattg cacagttaat tcagcatatt catgataagg ggccggtacg
     9661 ttcagccgat tttgagcatc ctcgtaaagg tgcaagcggc tggtgggaat ggaagccgca
     9721 taaacggcat ctggaaggtt tatttactgc cggaaaggtg atggtgattg aacggcgcaa
     9781 cttccagcgc gtttatgatt taacccaccg tgtcatgcct gactgggatg atgagcgcga
     9841 tctcgtttcg caaacagaag cagaaatcat catgctggat aacagtgcgc gtagcctggg
     9901 aatattccgc gaacagtggc tggcagatta ctatcggctg aaacgtccgg cactggcggc
     9961 gtggcgcgaa gcgagggctg aacagcagca aatcattgct gtgcatgttg aaaaattggg
    10021 caatctttgg ctgcatgctg atttgctgcc gctactcgag cgtgcgctgg ccggaaagct
    10081 cactgcaacg cacagcgcgg tactttcgcc ttttgatcct gttgtctggg atcgcaaacg
    10141 cgcagagcag ctttttgatt ttagctaccg gctggagtgc tataccccag cgccgaaacg
    10201 ccagtatggc tattttgttc tgccattatt acatcgtggg caattagttg ggcgaatgga
    10261 tgccaaaatg catcgccaga caggcatcct tgaagttatc tctctgtggt tacaggaagg
    10321 cattaaacca acgacaatgc tgcaaaaagg gttacgtcag gcgattactg atttcgcgag
    10381 ctggcagcag gcaacgcggg tgacattagg acgctgcccg caaggtctct ttactgattg
    10441 ccgcactggc tgggaaatag accccgtcgc ataaatgatt atgataagat ttatgcattc
    10501 ataacccgat ccatctggag gaactatgga tcatcgtttg cttgaaatca ttgcctgccc
    10561 ggtttgcaac ggaaaacttt ggtataacca ggaaaaacaa gagcttattt gcaaactgga
    10621 taacctggct ttcccgctcc gcgatggcat tccagtgttg ctggaaacgg aagcccgcgt
    10681 gctgactgct gatgagagta aatcatgagt tttgtggtca ttattcccgc gcgctacgcg
    10741 tccacgcgtc tgcccggtaa accattggtt gatattaacg gcaaacccat gattgttcat
    10801 gttcttgaac gcgcgcgtga atcaggtgcc gagcgcatca tcgtggcaac cgatcatgag
    10861 gatgttgccc gcgccgttga agccgctggc ggtgaagtat gtatgacgcg cgccgatcat
    10921 cagtcaggaa cagaacgtct ggcggaagtt gtcgaaaaat gcgcattcag cgacgacacg
    10981 gtgatcgtta atgtgcaggg tgatgaaccg atgatccctg cgacaatcat tcgtcaggtt
    11041 gctgataacc tcgctcagcg tcaggtgggt atggcgactc tggcggtgcc aatccacaat
    11101 gcggaagaag cgtttaaccc gaatgcggtg aaagtggttc tcgacgctga agggtatgca
    11161 ctgtacttct cacgcgccac cattccttgg gatcgtgatc gttttgcaga aggccttgaa
    11221 accgttggcg ataacttcct gcgtcatctt ggtatttatg gctaccgtgc aggctttatc
    11281 cgtcgttacg tcaactggca ggcaagtccg ttagaacaca tcgaaatgtt agagcagctt
    11341 cgtgttctgt ggtacggcga aaaaatccat gttgctgttg ctcaggaagt tcctggcaca
    11401 ggtgtggata cccctgaaga tcttgagcgc gttcgcgctg aaatgcgcta atttcacttc
    11461 acgacacttc agccaatttt gggaggagtg tcgtaccgtt acgattttcc tcaatttttc
    11521 ttttcaacaa ttgatctcat tcaggtgaca tcttttatat tggcgctcat tatgaaagca
    11581 gtagctttta tgagggtaat ctgaatggaa cagctgcgtg ccgaattaag ccatttactg
    11641 ggcgaaaaac tcagtcgtat tgagtgcgtc aatgaaaaag cggatacggc gttgtgggct
    11701 ttgtatgaca gccagggaaa cccaatgccg ttaatggcaa gaagctttag tacgcccgga
    11761 aaagcccgac aactggcatg gaaaaccacc atgctggcaa gaagtgggac tgtccgtatg
    11821 ccgactattt atggtgtgat gacgcatgaa gaacaccccg gccctgatgt cctgctactg
    11881 gagcggatgc gtggtgtttc ggtggaggca ccagcccgaa caccagaacg ctgggaacag
    11941 ctcaaagacc aaatcgttga agccttactg gcctggcacc gtcaggacag tcgcggttgc
    12001 gtcggcgcgg tcgacaatac tcaggaaaat ttctggccct catggtaccg gcaacatgtt
    12061 gaagtgctat ggaccacgct caatcagttc aataacaccg gtctgacgat gcaggataag
    12121 cggatcctgt ttcgcactcg cgaatgtctc ccggcattat ttgaaggctt taacgacaat
    12181 tgtgtgttga ttcacggtaa cttctgttta cgcagcatgc tgaaagattc gcgcagcgat
    12241 cagttactgg cgatggtcgg gccgggacta atgctttggg caccgcgaga atacgaactg
    12301 ttccgactaa tggataattc tctggcggaa gatttgctct ggagttacct gcaacgcgcg
    12361 ccagtggcgg agtcgttcat ctggcggcgt tggttgtatg tgttatggga tgaagttgcg
    12421 caactggtta ataccggacg atttagtcgg cgcaacttcg atctggcatc aaaatcactc
    12481 ttgccatggc tcgcctgacg gacctttcag ccactgccag atgcggccaa gcgtttcata
    12541 gccaacacga tcgctatgca tcagccagac cggagaaggg attgcccgtt cccacggatt
    12601 aagcggcgag tcgatggcca actgattcgc cggggctggt agcggattta actcttcctg
    12661 ctgaaaaaag atcatcgccc gcggcagatg tgatgcggag gtcaccagca ggaaaggggc
    12721 gtcaccaatc gcctgtttca ctgccgcagc ttcttcttcg gtatcttttg gcaaatccag
    12781 ggtgataatt tgctcgcgcg gcacgcccag cgattgcgca actctggcac ctacttccgc
    12841 tgtactcacc gtattggttt ttgctacgcc tcccgtgaag atcagttttg atcccggatt
    12901 ttcgcgccat aagcgaatac cttcattcag gcgtggcaga ctgttattga ttaaattaga
    12961 gctcggtgcc cactgcgggt tccaggtata accacctccg agcaccacga tatagtccac
    13021 tttttgcgaa ttattccatg tcggataggt gctttcgatg ggacgcagca ggcggtcggc
    13081 taccggttgc aaactcaata acaagagtgc cagccaccca atgctaataa aaatcttcgc
    13141 ggttttttga aaacggctaa accacagcag cgccaggcca gcgccaataa ttaacaacat
    13201 cagcggaagt ggcagcaaca tattgccaat cacttttttc agtgtaaaaa gcatcctttc
    13261 tggttccttt tttaaccata tagcaaggga tcgccagcga tattacacca gacgggttca
    13321 ttctcgccgt ggctgtgaca aaatagcgtc tttatcagaa aacgtcttag ccaaccggtg
    13381 gagaaagcaa tgcaggatcg caattttgat gatattgcgg aaaagttttc ccgtaacatt
    13441 tacggcacca ccaaagggca gcttcgacag gctattctgt ggcaggatct cgatcgcgtg
    13501 ctggcggaaa tgggtccgca aaaactgcgt gtgctggatg cgggcggtgg agaagggcag
    13561 accgcaatca aaatggccga gcgcgggcat caggtcattt tatgcgatct ttctgcgcag
    13621 atgatcgacc gcgcaaaaca ggcggcagaa gcaaaaggtg tgagcgacaa catgcaattt
    13681 atacattgcg ccgctcagga tgttgcttcg catttggaaa cgcccgttga tctgatattg
    13741 ttccatgcgg tgctcgagtg ggtggccgat ccccgcagtg tattgcagac cctgtggtca
    13801 gtgttgcgtc caggcggcgt gttgtcgtta atgttctaca atgcgcatgg tttgttgatg
    13861 cataacatgg tcgccgggaa ttttgattac gtgcaggcgg gaatgccgaa aaagaaaaaa
    13921 cggacgcttt cgccagatta tccacgcgac ccggcgcagg tttatctgtg gctggaagaa
    13981 gctggttggc aaattatggg taagacaggc gttcgcgtgt ttcatgatta tctgcgcgag
    14041 aagcaccagc agcgcgactg ctatgaagca ttacttgaat tagaaacgcg ttattgccgt
    14101 caggaaccgt atattaccct ggggcgttat attcatgtca ccgcgcgcaa accgcagagc
    14161 aaggataaag tatgagtgaa ttttcccaga cagtccccga actggttgcc tgggccagaa
    14221 aaaatgactt ctccatctcg ctgccggtag accgactctc ttttctgctg gcggttgcca
    14281 cgctgaacgg cgagcgtctg gatggtgaga tgagtgaagg cgagctggtg gatgcattcc
    14341 gccatgtgag tgatgcgttt gagcaaacca gcgaaaccat cggcgtgcgc gccaataacg
    14401 cgatcaacga catggtgcgt caacgtctgc tgaaccgctt taccagcgag caggcggaag
    14461 ggaacgcaat ttaccgtctg acgccgctcg gcatcggcat tactgactac tacatccgtc
    14521 agcgcgagtt ttctacgctg cgtctttcta tgcagttgtc gattgtggcg ggtgagctca
    14581 aacgcgcagc agatgccgcc gaagagggcg gtgatgaatt tcactggcac cgtaatgtct
    14641 atgcgccact gaaatattcg gtagcagaaa ttttcgacag tatcgacctg acgcaacgtc
    14701 tgatggacga acagcagcag caggtgaagg acgatatcgc ccagttgctg aacaaagact
    14761 ggcgggcggc gatttccagc tgtgaattgt tgctttcgga aacttccgga acgctgcgtg
    14821 aattgcagga tacgctggaa gcggcaggcg acaaattgca ggctaatctg ttgcgcattc
    14881 aggatgcgac gatgacccat gacgatctgc atttcgtcga tcgtctggtg ttcgatctgc
    14941 agagcaaact cgatcgtatt atcagttggg gccagcaatc catcgacttg tggattggct
    15001 acgaccgcca cgtacacaaa tttattcgta ccgcgatcga tatggataaa aaccgcgtct
    15061 ttgctcagcg gttacgtcag tcggtacaaa cctattttga tgagccgtgg gcgctaactt
    15121 atgccaatgc cgatcgtctg ctggatatgc gtgacgaaga gatggcactg cgcgatgaag
    15181 aagtgactgg ggaacttcct gaggatctgg aatacgaaga gtttaacgag atccgcgaac
    15241 agctggcggc gatcatcgaa gaacaacttg ccgtgtacaa aaccagacaa gtgccgctgg
    15301 atcttggtct ggtggtacgc gaatatctgt cacagtatcc gcgtgcacgt cactttgacg
    15361 ttgcgcgtat tgttattgat caggcggtac gtcttggcgt agcgcaagca gatttcaccg
    15421 gactgccagc gaaatggcag ccgattaatg attacggagc caaggtacag gcgcatgtca
    15481 tcgacaaata ttgaacaagt gatgtcggtt aagctggcgc aggcgctggc gaatccgtta
    15541 tttccggcgc tggacagcgc cttacgttca ggccgccata ttggcctcga cgaactggat
    15601 aatcatgcat tcctgatgga ttttcaggaa tatctggaag agttttacgc gcgttataac
    15661 gttgagctta ttcgcgcacc ggaagggttc ttctatttac gcccacgttc caccactctt
    15721 atcccgcgtt ccgtcttgtc ggaactggat atgatggtcg ggaaaatcct ctgttatctc
    15781 tatctcagcc cggaacggct ggcgaatgag gggattttca cccagcagga actgtacgac
    15841 gaactgctca ccctggccga tgaagcaaaa ctgctgaaac tggtgaacaa ccgttcaacc
    15901 ggttcagacg ttgaccgtca gaagttgcag gagaaagtac gttcttcgct caaccgtctg
    15961 cgtcgtttag gcatggtgtg gtttatgggc cacgacagca gcaagtttcg cattaccgaa
    16021 tcggtgttcc gcttcggagc cgatgtgcgt gctggcgacg atccccgtga agcacagcgt
    16081 cgcctgattc gtgatggcga agcaatgccg attgaaaatc atctgcaact caacgatgaa
    16141 accgaagaga atcagccaga tagcggagag gaagaataat gattgaacgc ggtaaatttc
    16201 gctcactgac gctgattaac tggaacggct tttttgcccg aacttttgac cttgacgagc
    16261 tggtcacgac gctttctggc ggtaacgggg cgggtaaatc caccaccatg gcggcgttcg
    16321 ttacggcgct gatccccgac ctgaccctgc tgcatttccg taacactacg gaagccgggg
    16381 ccaccagcgg ttcgcgcgat aaaggtctgc acggtaagct gaaagcgggt gtctgttatt
    16441 cgatgctcga caccattaac tcgcgccacc agcgcgtggt ggtcggtgtg cgtctgcaac
    16501 aggttgccgg acgcgatcgt aaagtggata tcaaaccatt tgccattcag ggactgccga
    16561 tgtcggtgca gccgacacag ctggtgaccg aaaccctgaa cgaacgccag gcgcgcgtgc
    16621 tgccgcttaa cgagctgaaa gacaagctcg aggcgatgga aggcgtgcag tttaaacagt
    16681 tcaactccat taccgattac cactcgctga tgttcgatct gggcatcatc gcgcgtcgtc
    16741 tgcgctccgc atctgaccgt agcaaattct atcgtctgat cgaagcttcg ctgtatggcg
    16801 ggatctccag tgccattacc cgttctctgc gcgactacct gttgccggaa aacagcggcg
    16861 tgcgtaaagc gttccaggac atggaagcgg cgctgcgtga aaaccgcatg acgctggaag
    16921 cgattcgtgt cacccagtcg gaccgtgacc tgtttaagca tctgatcagc gaagctacca
    16981 actacgtggc ggcggactac atgcgtcacg ccaacgagcg ccgtgtccat ctcgacaaag
    17041 ccctggagtt tcgtcgcgag ttacatactt cgcgtcagca actggcggct gagcagtaca
    17101 aacacgtcga tatggcgcgt gagctggcag agcacaacgg tgccgaaggc gatctggaag
    17161 cggattatca ggcggccagc gatcacctga acctggtgca aaccgcacag cgtcagcagg
    17221 agaagatcga acgctacgaa gcggatctcg atgagctgca gatccgtctg gaagagcaaa
    17281 acgaagtggt ggcagaagcc atcgaacgcc aggaagagaa cgaggctcgc gcggaagctg
    17341 ccgaactgga agtggacgag ctgaaaagcc agcttgctga ctaccagcag gcgctggacg
    17401 tccagcaaac gcgcgcgatc cagtataacc aggcgattgc tgcgcttaat cgcgccaaag
    17461 aactgtgcca tctgccggac ttaaccgccg acagcgccgc cgaatggctg gaaaccttcc
    17521 aggcgaaaga gctggaagcg actgaaaaaa tgctctctct tgagcagaaa atgagcatgg
    17581 cgcaaaccgc gcacagccag tttgagcagg cttatcagct ggtggtggca atcaacggcc
    17641 cgctggcgcg taacgaggct tgggatgtcg cccgcgaact gctgcgcgaa ggggtcgatc
    17701 agcgtcacct ggcagagcag gttcaaccgt tgcggatgcg cttaagtgaa ctggaacagc
    17761 gtctgcgcga gcagcaagaa gccgaacgcc tgctggcaga cttctgcaaa cgtcagggca
    17821 agaattttga tattgatgaa ctggaagctc tgcatcagga gctggaagcg cgcattgcct
    17881 ctctttccga tagcgtgtct aacgcccgtg aagagcgcat ggcactgcgc caggaacagg
    17941 aacagctgca gtctcgcatt cagagtttga tgcagcgtgc gccggtttgg ctggcagcgc
    18001 aaaacagtct caaccagttg agcgagcagt gcggtgaaga gtttacctcc agccaggacg
    18061 tcaccgaata tctgcaacag ttgctggagc gtgagcgaga ggcgattgtt gaacgcgatg
    18121 aagtgggcgc gcgcaaaaac gctgtcgatg aagagatcga acgtttaagc cagcctggcg
    18181 gctctgaaga tcagcgtctg aacgcgctgg cggagcgttt tggcggcgtg ctgctgtcag
    18241 aaatttatga cgacgttagc ctggaagatg cgccgtactt ctcagcgctg tatggcccgt
    18301 cacgccacgc catcgtggtg ccagatctgt cacaggtaac tgaacacctg gaaggcttga
    18361 ccgattgccc ggaagatctc tatttgatcg aaggggatcc gcagtcattc gatgacagcg
    18421 tgttcagcgt tgatgagctg gaaaaagcgg tagtggtgaa aatcgccgat cgtcagtggc
    18481 gttattcacg tttcccggaa gtgccgctgt ttggtcgtgc tgcgcgtgaa agccgtattg
    18541 aaagcctcca tgccgagcgt gaagtgcttt ccgaacgctt cgccacgctc tcctttgatg
    18601 tacagaaaac ccagcgtctg catcaggcgt tcagccgctt tatcggcagt catctggcgg
    18661 tggcgtttga gtctgacccg gaagcagaaa tccgtcaact gaacagccgt cgcgtcgaac
    18721 tggagcgggc gttgagtaat catgaaaatg ataaccagca gcagcgtatt cagtttgagc
    18781 aggcgaaaga aggcgttacg gcgctgaacc gcattctgcc gcgtctcaac ctgttggctg
    18841 atgacagcct ggcggatcgc gtcgatgaaa tccgcgaacg tctggatgaa gcccaggaag
    18901 ccgcgcgttt tgttcagcag tttggcaatc aactggcgaa actggaaccg atcgtttcgg
    18961 tattgcagag cgacccggaa cagttcgaac agctaaaaga agattacgcg tactctcagc
    19021 agatgcagcg cgatgcccgt cagcaggcgt ttgccctgac ggaagtggtg cagcgtcgtg
    19081 cgcactttag ctattctgac tcggcagaaa tgctgagcgg taatagcgat ctcaacgaaa
    19141 agctgcgtga acgtctggaa caggcggaag cggagcgtac tcgcgctcgt gaagcgttgc
    19201 gtggtcacgc agcgcagttg agtcagtaca accaggtgct ggcttcgctg aaaagttctt
    19261 acgacaccaa aaaagagcta ctcaacgatc tgcaacgtga attgcaggat atcggcgtgc
    19321 gcgccgatag cggggcagaa gagcgggcgc gaattcgccg tgacgagctg catgcgcaac
    19381 tgagtaataa ccggtcccgc cgcaaccagc tggaaaaagc actgactttc tgcgaagcgg
    19441 agatggacaa tctgacccgc aaactgcgca agctggagcg ggattacttt gagatgcgcg
    19501 agcaggtagt gaccgccaaa gcgggctggt gtgcagtgat gcgcatggtg aaagataacg
    19561 tcgttgaacg gcgcttacac cgtcgcgagc tggcttatct ctccgctgat gatttgcgct
    19621 ctatgtcgga taaggcattg ggtgcgctgc gtctggcggt ggcggataac gaacatctgc
    19681 gcgacgtgct gcgcatgtcg gaagatccaa aacgtccgga acgtaaaatt cagttcttcg
    19741 tggcggttta tcagcatctg cgtgaacgta ttcgtcagga tattattcgt accgatgatc
    19801 cggtggaagc catcgagcag atggaaatcg aacttagccg actgaccgag gagttaacct
    19861 cccgcgaaca gaaactggcg atcagttccc gcagcgtggc gaacatcatt cgcaaaacca
    19921 ttcagcgcga acagaaccgt atccgtatgc tcaaccaggg gttgcagaac gtatcgtttg
    19981 gtcaggtgaa cagcgtacgt ctgaacgtga acgtgcgtga aacgcacgcc atgctgctgg
    20041 atgtgctctc tgaacagcac gagcagcatc aggatctgtt taacagcaac cgtctgacct
    20101 tctcggaagc gctggcgaaa ttgtatcagc gtcttaatcc gcagattgat atggggcagc
    20161 gcacgccgca gaccattggc gaagaactgc tggattaccg caactatctg gaaatggaag
    20221 ttgaggttaa ccgtggttcc gatggctggt tgcgcgcaga gtctggtgca ttgtcgaccg
    20281 gtgaggcgat tggtaccggt atgtcgattc tggtgatggt ggtacaaagc tgggaagatg
    20341 aatctcgccg cctgcgcggt aaagatatct ctccttgccg cctgctgttc ctcgatgaag
    20401 cagcgcgact ggatgctcgt tctatcgcca cgctgtttga attatgtgaa cgtttgcaaa
    20461 tgcagctcat catcgcagcg ccggaaaata tcagcccgga gaaaggcacc acctataaac
    20521 tggtgcgtaa agtcttccag aataccgaac acgttcacgt cgtcggcctg cgaggatttg
    20581 cgccgcaact ccctgaaacg cttccaggaa gtgacgaagc gccttctcag gcgagttaaa
    20641 attaaggcgg cagcaatgcc gccttttctt tttccgaaaa ctccgtttct gcactaaaaa
    20701 agtggcacat tacggcgcgc ttttctttaa acttctttac attaggttat gtaaaaacgt
    20761 atcggcgttt atatactgaa gataagcctg atgagtaaca ggcttgctcg tcatactttc
    20821 gtgagtattg gcgttgtaca ggcaagtcgt aaaataacag cctggctatt cagagtatga
    20881 taaaaacagg gggcgaggga tgttgcttaa tatgatgtgt ggtcgtcggc tgtcggcaat
    20941 cagtttgtgc ctggccgtaa cattcgctcc actgttcaat gcgcaggccg atgagcctga
    21001 agtaatccct ggcgacagcc cggtggctgt cagtgaacag ggcgaggcac tgccgcaggc
    21061 gcaagccacg gcaataatgg cggggatcca gccattgcct gaaggtgcgg cagaaaaagc
    21121 ccgcacgcaa atcgaatctc aattacccgc aggttacaag ccggtttatc ttaaccagct
    21181 tcaactgttg tatgccgcac gcgatatgca acccatgtgg gaaaaccgtg atgctgttaa
    21241 agccttccag caacagctgg cagaggtggc gattgccgtt ttccagccgc agtttaataa
    21301 atgggtagag ttactgaccg atcctggtgt taacgggatg gcacgcgacg tagtgctctc
    21361 tgatgcgatg atgggctatc tccatttcat tgcaaatatt ccggtcaaag gcactcgctg
    21421 gctatatagc agtaaacctt atgcacttgc aacgccgccg ctctcggtga ttaaccaatg
    21481 gcagctggcg ctggataaag gtcaattgcc tacgtttgtt gcaggactgg caccgcagca
    21541 tccgcaatat gcggcgatgc atgaatcgtt actggcctta ctcagtgaca ccaaaccgtg
    21601 gccccaactg accggcaaag caacgttgcg cccagggcag tggagtaacg acgtaccggc
    21661 gttgcgcgaa atattgcaac gcacaggcat gttggacggg gggccgaaaa ttactctacc
    21721 tggcgatgac acgccaactg acgcggtagt cagcccatcc gctgttactg ttgaaacagc
    21781 agaaactaag ccgatggaca agcaaacgac gtctcgtagt aaacctgcgc ctgccgttcg
    21841 cgccgcctac gataatgaac tggtggaagc cgttaaacgt tttcaggcat ggcaaggatt
    21901 gggggcagat ggtgctattg gcccggcaac acgtgactgg ttaaacgtaa cgcccgccca
    21961 gcgtgctggc gtgttggctc tcaacatcca gcgattgcgc ttgctgccaa cagagctttc
    22021 taccgggatc atggttaaca ttccggccta ttcgctggtc tactatcaga acggcaatca
    22081 ggtgctggat tcgcgagtca ttgtcggtcg ccccgatcgc aaaacgccga tgatgagcag
    22141 tgcccttaac aacgtagtgg taaacccgcc gtggaacgta cctccaactc tggcacgcaa
    22201 agatattctg ccaaaagtgc gcaacgatcc gggatatctc gaaagccatg gctatacggt
    22261 gatgcgcggc tggaacagca gagaagcgat tgacccatgg caggttgact ggtctacaat
    22321 cacggcctcg aatttaccgt tccgcttcca gcaggctcca ggcccacgga actcgctggg
    22381 gcgctataaa ttcaatatgc cgagttcaga ggccatttat ttgcatgaca cgccgaacca
    22441 caatctgttc aagcgtgata cacgcgcatt gagctcaggc tgtgtacgag tgaataaagc
    22501 ttccgatctg gcgaatatgc tgttgcagga tgcaggctgg aatgacaaac gtatttctga
    22561 tgcgctgaag cagggtgata cacgttacgt caatattcgg cagtcgattc cggtgaatct
    22621 ctactacctg acggcctttg ttggtgcaga tggtcgtacc cagtatcgta cagatattta
    22681 caattatgat ctgcctgcgc gatccagctc gcaaatcgta tcgaaagcgg aacaattaat
    22741 caggtaaatg aagtagttcg gggaaatcag ttgtcgtaat aattagcatg attgggggcg
    22801 attctctgca gcccccgtca ctgctggggt tgagtcatct tgacgtctgc tttacgggcg
    22861 gttaaggtgc ctcttgtgcg ccagaagtgc atataaacga taacattgac ctgtagactt
    22921 gattatcatg gacaaattcg acgctaatcg ccgcaaattg ctggcgcttg gtggcgttgc
    22981 actcggtgcc gccatcctgc cgacccctgc gtttgcaaca ctctctaccc cacgcccgcg
    23041 cattttgaca ctcaataatc ttcataccgg agagtcaatc aaagcggagt ttttcgatgg
    23101 cagaggctat attcaggaag aattggcaaa acttaaccat tttttccgcg attaccgcgc
    23161 gaacaaaata aagtccatcg acccaggatt attcgaccag ttgtatcgcc tgcaagggtt
    23221 gttaggcacg cgcaaaccgg tgcaactcat ttccggttat cgttctattg ataccaacaa
    23281 tgaactacgc gcccgcagcc gtggagtagc gaagaaaagc tatcacacta aaggccaggc
    23341 gatggatttc catattgaag gtatcgcgtt aagcaatatt cgcaaagccg cgttatctat
    23401 gcgcgcaggt ggtgtaggat attatccacg tagtaacttt gtgcatattg ataccgggcc
    23461 agcacggcac tggtagtaat tgcttaacga aacaggggca gtatgaacta tcgtattatt
    23521 ccggtcaccg cattctccca gaactgttca ttaatctggt gtgaacaaac ccgtctggcc
    23581 gcactggtcg atcctggcgg cgatgcggaa aaaatcaaac aggaagttga tgacagcggc
    23641 ctgacactga tgcagatcct gctgacgcat ggtcatctgg accacgttgg cgcagcggcg
    23701 gaactggcgc aacattacgg cgtgccggtt tttggcccgg aaaaagaaga tgagttctgg
    23761 ctgcaaggct tgcctgcgca aagtcgtatg tttggtctgg aagagtgcca gccgctgacg
    23821 ccagatcgtt ggctgaacga aggcgatacc atcagcatag ggaatgtgac tttacaggtg
    23881 ttacattgcc ctgggcatac gccgggtcat gtcgtgtttt ttgatgatcg ggcaaagctg
    23941 ctgatttctg gcgatgttat tttcaaaggc ggagtagggc gcagtgactt cccgcgtggc
    24001 gatcataatc aactgatttc ttcaatcaaa gataaattgc tgccactggg ggatgacgtg
    24061 acatttattc cgggtcacgg accattatcc acacttggtt atgaacgcct gcataatccc
    24121 ttcctgcaag acgaaatgcc cgtctggtaa ggcacataaa aaagcccgct tttaatgcgg
    24181 gcctggattt ctggcaaagt gcgctttgtt tatgccggat gcggcacgag cgccttatcc
    24241 ggcctacaaa atcgtgcaaa ttcaaaatat tgcaggggac gcgtaggcct gataagcgta
    24301 gcgcatcagg caatgttgcg tttgtcatca gtctcagccc gcttttcagc gggcttcatt
    24361 gtttttaatg cttacagcac tgccacaatc gcttcgcaca gcggagccat gttatctggt
    24421 gtcatcccgg ccacatttac gcgaccagaa gcaaccgcat atacgccaaa ctcttcgcgc
    24481 agacgcagca cttgttcttt tgtcaggcca ctgaaggaga acatgccgtt ctgtttgatg
    24541 ataaagctga agtcgcggtt tgcgcctttt tcctgcagcg tattgacgaa caactgacgc
    24601 atacgctgaa tacgctggcg catatcagtc agctcttgtt cccaaatcgc acgtaacgca
    24661 tcgttgctca ggatggtggc aacaacagaa gcgccgtgtg ctggtgggtt agagtagtta
    24721 gcgcgaatcg ccgctttcat ttggctgaat gcgcgatcaa cggtttcact gtcggcagca
    24781 accagagtac aagcgccaac acgctcgttg tacaggccaa agtttttaga gtaggaactg
    24841 gcaacaatca gctctttatg catagccgcg aaagcgcgca gtccttcagc atcttcttcc
    24901 agaccacggg caaaaccctg gtaagcgaag tcaaacagcg gtaaccagcc tttctcaacg
    24961 gagagttgtg ccagtgtctg ccattgttcc agcgtagggt cgataccggt tgggttatgg
    25021 cagcagccat ggaacagcac tacgtcgcca gcctgagctt cattcaggct gttaatcagt
    25081 gcatcgaagt caagagtgtg attttccgca tcataataag cgtattcacg aacttccaga
    25141 cctgcagagt taaagacgct cttatggttc ggccagcttg ggttgctcac ccacactcgc
    25201 ttaacgctgg tattttttgc caggaaatcg gcagctacgc gtagtgcgcc agtgcccccc
    25261 ggagtctgtg ccgtgcgagc acgtttgtca ttgatcaggg cgctaccttt accaaacagc
    25321 agttcctgag tgcagcgacc aaattcaggg atgccgtcaa tgccgaggta atttttggtg
    25381 gtttcatttt cgagcagata ctgttcagcc tttttcacgc tggtcagtac cggggttttg
    25441 cccgtctcat ctttatagac accaatcccg aggttaattt tgccgggacg ttcatcggca
    25501 cgaaacagat cggccaggcc cagaatcggg tcggcaggag cggcggtaat gttctcaaac
    25561 atgacgaggt tccattatgg ttacagaagg gaagtccgct atcagggtaa cgggagattt
    25621 acaaaattcc aactattact gatgaaaacg caggctgttt ttgcaagacg tgagattgct
    25681 ctggaaggta taaaaaaaac aggaccaaag tcctgttttt tcggcattta acaaagaggt
    25741 gtgctattag aactggtaaa cgatacccac agcaacggtg tcgtctgaac ctacgcccag
    25801 tttgttgtca gaatcgatct ggttgatgat gtagtcaaca taggtggaca tgtttttgtt
    25861 gaagtagtag gttgcgccca cttcaaagta gttcaccaga tcaacatcac cgataccttc
    25921 tacgtctttc gctttagatt tggtgtaagc gatggacgga cgcagaccga aatcgaactg
    25981 gtattgcgca actaacagaa cgtcttgcgt tttgttggcg aagccgctgg tgtttgtaaa
    26041 tttattagtg atcggcgtag cgttacgggt ttcaccgtag ttcgctgcca ggtagatgtt
    26101 gttcgcgtcg tacttcagac cagtagccca ctgttcagct tttttaccgt tgccaagagg
    26161 ttgagcttct tgcaggttgg tacggtcagc tgcaccataa gcaccaacga taccaaagcc
    26221 ttcgtattcg tagctgatag aaccgccaac accgtcgccg ttagaacggc gtgcagtgtc
    26281 acgctcgttt ttacccaggt actgaacagc gaagttcagg ccatcaacca gaccaaagaa
    26341 gttggagtta cgataggtag caacgccgcc aacacgacca acgaagaagt catcgctgta
    26401 tgcagtatca ccaccaaatt ctggcagcat atcggtgtaa cccagtgcat cataaaccac
    26461 accgtagtta cggccgtaat cgaaagaacc aacgtcagcg tatttaagac ccgcgaatgc
    26521 cagacgcgtt ttgttaccag tttgagcgtc agcgccttca gagttgttac cctggaagtt
    26581 atattcccac tgaccataac cggtcagatc ggaattgatt tgagtttccc ctttaaaacc
    26641 aagacgggca taggtcatgt cgccattgcc accgtaactg ttttcaccgt tacccttgga
    26701 aaaataatgc agaccaacag ctttaccgta cagatctact ttgttgccat ctttgttata
    26761 gatttctgca gcgtttgcag tacctgctac taacagagca gggacgatca ctgccagaat
    26821 attgcgcttc atcattattt attaccctca tggttttttt tatgacacct gccactgccg
    26881 tcaataagtt ctgtcaataa aaatttacgg aactattgat gagagtttgg tgtctttatg
    26941 tgtctgcagg catctttcca ttcaaactaa cgtttcgcta ccgtgaaagt gctacaaaga
    27001 taaagattta gtttcaaaaa gaaaaaatat gtaaccaaaa gtaaaaatta aggaactttg
    27061 tgaacaccgt catatttcca tagaaacgtg atgatattta cagcaatttt tatctattta
    27121 tatgatttac ttatatttaa attaactaaa cggaaatttt gtttctgatg gaaactttat
    27181 cgacctggca caaaatcctc tttcagacat ccagaatgcc aaaaaatagt atgaaaattg
    27241 tgctatcagc taagaaaaaa ctaatccgca ataagaatgc gggttatttt ttgtgcgtaa
    27301 atgttgcaat gtaatattcc gtaacaggat gatcgttatg gcggggttgc aaatagattg
    27361 cttgacttaa caaacagata acttgacaga aaggataaaa aagagccagc aggcgctggc
    27421 tctttgagga agaaaatcag aagctggcgt tgcgcggagt acgtgggaac ggaatcacat
    27481 cacgcacgtt ttgcacgcca gttacgtaag caatcaggcg ttcaaaacca agaccgaaac
    27541 ctgaatgcgg aacagtaccg tagcgacgca gatcgcgata ccaccagtaa tcttctttat
    27601 tcaggcccat ttccagcata cgctcgtcca gaacgtccag acgttcttca cgctgggagc
    27661 caccaatgat ctcaccgatg cccggagcca gaacgtccat agccgcaacg gttttaccgt
    27721 cttcgttgag gcgcatatag aacgctttaa tatctttcgg atagttttta accactaccg
    27781 gtgctttaaa gtgttcttcc gccagataac gctcatgctc agaagagaga tcgacgcccc
    27841 agtaaaccgg gttttcaaac ttcctgccgc agttttcgag aatggtcact gcgtcggtgt
    27901 aatccacctg cgcgaaatcg gcctcaatga agcgttccag acgtgaaacg gcatctttat
    27961 ctacgcgttc agcgaagaat ttcatgtcgt ctgcgcgttc ttcgagaacc gctttgaaga
    28021 catatttcag catggcttca gccagacccg cgatatcgtt caggttagca aacgccactt
    28081 ccggctccag catccagaat tccgccaggt gacggctggt gttggagttt tcagcacgga
    28141 aagtcgggcc gaaggtataa attttggata atgcgcaagc gtaggtttcg ccgttcaact
    28201 ggccagatac ggtcaggaaa gactctttac caaagaagtc tttgtcgaaa tccactttgc
    28261 cctgatcgtt acgcggcagg ttttccagat ccagcgtaga aacgcggaac atttcgcctg
    28321 caccttcggt atcagatgcg gtaatcagtg gcgttgaaac ccagaagaat ccctgctcgt
    28381 taaagaagcg atgcagcgcc tgcgccagcg tatggcgaac gcgcgcgacg gcaccaatca
    28441 ggtttgtgcg cggacgcagg tgagcgactt cacgcagata ctcaatgctg tggcgttttg
    28501 ccgccatcgg gtaagtgtct ggatcttcaa cccaaccagc aacttcaacc ttgctggcct
    28561 gaatttcaaa ttgttgcccc tggcccggcg atgccacgac tttacccgtc acaatgaccg
    28621 agcagccggt ggtcagacgc aggacgtctt cattgtaatt gggcagagaa ttattgatga
    28681 cagcctgtac aggatcaaag caggaaccgt cataaacggc gaggaaggag atgccagctt
    28741 ttgaatctcg gcgggtacgt acccatccgc gtacggtgac ttcgctgtca acggctacgc
    28801 ggccctggag tacgtcggct acaggcacaa cgctcataat attctctctg ttaatagtcg
    28861 gaaaaaataa acacttgtcc acccgtaaat gggggggata cctatgttac ctggcatctg
    28921 caatcagaca agcagaattc gcaaatgcag cgaaagattt cggaattaaa ggtaaaaaaa
    28981 gggagccgat tagctccctg atgatattaa ctggcttttt taatatgcgg aaggtcgaac
    29041 gctttgcgca gcgcccgaac aaacgcttta tcatggcaga tagttttgcc agggctgtca
    29101 gaaagtttcg ccaccggttt accgttacac tctaccaact taatcacaat attcaggggt
    29161 tttacctggg ggatatcgca ggtcaggcga gtcccaatac caaaacttaa ttgcacgcgg
    29221 gaagagaagt ggcggtatag ctcaaccgct ttgcgtaaat ccagattgtc agagaaaacc
    29281 agcgttttac tctgtggatc aattcccagc ttttcataat gtgcaatggc tttttcaccc
    29341 cattcaaccg ggtcgccaga gtcatgacgc aggccctgat accgactagc gaactcgaca
    29401 ccgaaatcac gcaggaaagc atccatagtg atgcagtcgg ttaatgcaat gccaagttgg
    29461 tcgggatact cttccagcca ggcagcaagt gcagctcgct ggctgttggc tagatccggg
    29521 ctgatttgct gatgtgcctg gaaccattcg tgtgcctgtg ttcccatcgg cgtgagggaa
    29581 agccgacgcg ccagatcgta gttgctggtg cccacgaacc aggattcctg ttgcagacgc
    29641 ttaacgatgg tttcttgtac ttcgcgagaa aaacggcgac gggtgccaaa atccatcaga
    29701 tggaagcgcg acatatcaag accggcggtt aacgccgaga agtcgactaa tttgctttcc
    29761 agcgtgtcga gggcttgcgc aacgtcggcc tgcggtgagc gatagcgatg taccatttca
    29821 ctgataaccg ccagcaaagg aacttcccag aggatgactt cacgccacgg accgcttaag
    29881 cgaatatcca gcttgccatt atcgttggac acggtgactt gttccgggtt aaagcggaac
    29941 tcgcgtaacc agttaagata gtcggcctta aagaaaggca gggcagagag ccactgatat
    30001 tcatcatcct gcaggcgcag gtgctgcatc gcctgaatct gttcacgaat agcatcggca
    30061 taaataccca gcagatcgtc acctcggcaa cgaaactccg ccgcgacatg cacatcgtaa
    30121 tagtgatgaa acacggcttg ctgcatatgc aacttataag catctgtatc cagcaacgag
    30181 tgcagaacag gagaagcgaa ttgtgtcata ggtgcgcagt agcgtcctcg tacaggagcg
    30241 tttagtacaa taaacatctt caggaactgc tggagtatac cttgtttcgc aatttattga
    30301 accccgatca caccatatgc cacctttctg gtcgatggca ttacgcggtg tatgttataa
    30361 aaatgtagca ataaaggcgt ttgtacctga aaagatgaag attctgcata gcgcgattta
    30421 cgcaacagga atagactgaa caccagactc tataaaagat gctaaaggtt atttatgact
    30481 caacagccac aagccaaata ccgtcacgat tatcgtgcgc cggattacca gattactgat
    30541 attgacttga cctttgacct cgacgcgcaa aagacggtcg ttaccgcggt cagccaggct
    30601 gtccgtcatg gtgcatcaga tgctccactt cgtctcaacg gcgaagacct caaactggtt
    30661 tctgttcata ttaatgatga gccgtggacc gcctggaaag aagaagaggg cgcactggtc
    30721 atcagtaatt tgccggagca ttttacgctt aagatcgtta atgaaataag cccggcggcg
    30781 aataccgcgc tggaagggct ttatcagtca ggcgatgcgc tttgcaccca gtgtgaagcc
    30841 gaaggtttcc gccatattac gtattatctc gaccgcccgg acgtgctggc gcgttttacc
    30901 accaaaatta ttgccgataa aaccaaatat cccttcctgc tttccaacgg taaccgcgtt
    30961 gcgcaaggtg aactggaaaa cggacgccat tgggtacagt ggcaggaccc gttcccgaaa
    31021 ccgtgctacc tgtttgcgct ggtggcaggc gactttgatg tactgcgcga cacctttacc
    31081 acgcgttctg gtcgcgaagt ggcgctggag ctgtacgtcg atcgcggcaa ccttgatcgc
    31141 gcgccgtggg cgatgacctc gctgaaaaac tcaatgaaat gggatgaaga acgcttcggc
    31201 ctggagtatg acctcgacat ctatatgatc gtcgcggtgg atttcttcaa tatgggcgca
    31261 atggagaata agggtctgaa tatctttaac tccaaatatg tgctggcccg caccgacacc
    31321 gccaccgaca aagattacct cgatattgaa cgcgttatcg gccatgaata tttccataac
    31381 tggaccggta accgagtgac ctgccgcgac tggttccagc tcagcctgaa agaaggttta
    31441 accgtcttcc gcgatcagga attcagctct gaccttggtt cccgcgcagt taaccgcatc
    31501 aataatgtgc gcaccatgcg cggattgcag tttgcggaag acgccagccc gatggcgcac
    31561 ccaatccgcc cggacatggt cattgagatg aacaacttct acaccctgac cgtttacgag
    31621 aagggcgcgg aagtgatccg tatgatccac accctgctgg gcgaagaaaa cttccagaaa
    31681 gggatgcaac tctatttcga gcgtcatgat ggtagcgcag cgacctgtga cgactttgtg
    31741 caggcgatgg aagatgcgtc gaatgttgat ctctcccatt tccgccgttg gtacagccag
    31801 tccggtacgc cgattgtgac cgtcaaagac gactacaatc cggaaactga gcagtacacc
    31861 ctgaccatca gccagcgcac gccagccacg ccggatcagg cagaaaaaca gccgctgcat
    31921 attccgtttg ccatcgaatt gtatgacaac gaaggcaaag tgatcccgtt gcagaaaggc
    31981 ggtcatccgg tgaattccgt gctgaacgtc acccaggcgg aacagacctt tgtttttgat
    32041 aatgtctact tccagccggt gcctgcgctg ctgtgcgaat tctctgcgcc agtgaaactg
    32101 gaatataagt ggagcgatca gcaactgacc ttcctgatgc gtcatgcgcg taatgatttc
    32161 tcccgttggg atgcggcgca aagtttgctg gcaacctaca tcaagctgaa cgtcgcccgt
    32221 catcagcaag ggcagccgct gtctctgccg gtacatgtgg ctgacgcttt ccgcgcggtg
    32281 ctgctcgatg agaagattga tccggcgctg gcggcagaaa tcctgacgct gccttctgtc
    32341 aatgaaatgg ctgaactgtt cgatatcatc gacccgattg ctattgccga agtacgcgaa
    32401 gcactcactc gtactctggc gactgaactg gcggatgagc tgctggctat ttacaacgcg
    32461 aattaccaga gcgagtaccg tgttgagcat gaagatattg caaaacgcac tctgcgaaat
    32521 gcctgcctgc gcttccttgc ttttggtgaa acgcatctgg ctgacgtgct ggtgagcaag
    32581 caataccatg aagcaaacaa tatgaccgat gcgctggcag cgctttctgc ggcggttgct
    32641 gcacagctgc cttgccgtga cgcgctgatg caggagtacg acgataagtg gcatcaggac
    32701 ggtctggtga tggataaatg gtttatcctg caagccacca gcccggcggc gaatgtgctg
    32761 gagacggtgc gtggtctgtt gcagcatcgc tcatttacca tgagcaaccc gaaccgtatt
    32821 cgttcgttga ttggtgcgtt tgcgggcagc aacccggcag cgttccatgc cgaagatggc
    32881 agcggttacc agttcctggt ggaaatgctt accgacctca acagccgtaa cccgcaggta
    32941 gcttcacgtc tgattgaacc gctgattcgc ctgaaacgtt atgatgccaa acgtcaggag
    33001 aaaatgcgcg cggcgctgga acagttgaaa gggctggaaa atctctctgg cgatctgtac
    33061 gagaagatca ccaaagcact ggcttgatac ttttgagcca aaatgcctga tgcgacgctt
    33121 gacgcgtctt atcaggccta caactccgtt gaaattgctg gttttgtagg ctggataagg
    33181 cgttcacgcc gcatccggca gccgtgccca actgcctgat gcgatgcttg acgtgtctta
    33241 tcaggcctac aagtccgttg aaaatgctgg ttttgtgcgc cacatccggc atattaacca
    33301 tgacgccgaa tcagttgttc agaatgccca cgccgcatta cccgttgcaa tacttccgct
    33361 tccagctccg ctaatcgcac cgaccctaat cgacgcggac gggggatatc cactgtcaaa
    33421 tccagaccaa tttttccctc ttcaattaac aacacccggt cagccattgc taccgcttcg
    33481 ctcacatcat gcgtcaccaa cagtacggta aagccgtgct gctgccaaag tgacacaatc
    33541 aaatcctgca tctcgagtcg cgttaaggcg tccagtgccc cgagcggttc atcaagcaac
    33601 aataaacccg gtcgatgaat cttgccagcg ccacacgctg tttctgcccg ccagaaagtg
    33661 cggcaggcca ttccccggcg cgattctcca gtcctactgc agccaacgcc cgacgcgcgg
    33721 catcgcgcca ctggccttta aggcctaacc caacgttatc aatcaccgat ttccacggca
    33781 gcagacgcgc atcctgaaac atcattcgcg tgtcttcctg aatctcagcc agcggtgtgg
    33841 tgcccgctaa cacatcgcct gcggttggcg tttccagacc tgccagcagg cgcagcaggg
    33901 tacttttgcc accaccgctg cggcccacca ccgccacaaa ctgacctgcc ggaatatgta
    33961 aatccagttg gttcaggacg atattttccg cgtaatgttt gcttactgca ttgagcaaca
    34021 atggtgtgcc ctggttcaga cgagcagtat tcataccgtg gcctccttca aatgataagc
    34081 cgggttccag cgcaaccaga ggcgctctaa cagctgcgcg ctgacgtcag caagtttgcc
    34141 gagcagggcg taaagaataa tagcgactac caccacgtcc gtttgcaaaa actcccgcgc
    34201 attcatcgcc agataaccaa tgcctgaatt ggcagaaatg gtttcggcaa caatcagcgt
    34261 cagccacatc aggcccaacg caaaacgcac gccgaccata attgagggca gggcaccagg
    34321 caggatcaca tggataaaca gcggtatgcc ggataatcca tagctacgcg ccatttccac
    34381 cagaccgcga tcgatattac ggatgccatg ccaggtgttg atataaatgg ggaatagcgt
    34441 gccgagcgcc accagaaaga tttttgcgga ttcatcaatg ccaaaccaca aaatcaccag
    34501 cgggatcagc gccagatgcg gtacgttgcg caacatctga attgaggtat ccagcagccg
    34561 ttctccccag cgtgacagcc cgctaatcag tcccagaatc aagcccagcg atccgccaat
    34621 tgaaaagcca atcagcgccc gccaggaact gatcgccaga tgctgccaca attcgccgct
    34681 ggcggagagc gtccagaacg ccgtcaccac tccttccggt gaaggcaaaa tacgcgtcga
    34741 cagccagcca accgaggagg ccagttgcca caccgccacg atgcccaccg gtaaaaacca
    34801 gggggcaacg cgcaataacc acttcttcac tggcgttgcc atcatcgtgt tccttagctt
    34861 tgcgcgactt tacgggggat aaaatcattc gccaccgctt cgccttgcgg attcagcggc
    34921 tgcggctggg gaatttccgg aatggcgaca tccagatgtg ggaacagcaa ctcgccaacc
    34981 cgatacgctt cttccagatg cgggtagccc gaaagcacaa agctgtcgat gccaagcgcg
    35041 gcgtattcgt tgattcgcgc ggcgaccgta ggaccatcgc ccaccagcgc cgttccggca
    35101 ccgccgcgca ctaagccaac gcccgcccat aaattggggc tgatctccag attgtcgcgc
    35161 ttgccgttat gtagcgccgc cattcgctgt tgtcctacgg aatccgtccg ggcgaatgcg
    35221 gcctgtgctt tggcgatagt ttcatcatca agatgcgaga ttaaccgctc ggcggcctgc
    35281 cacgcttcgt cgttagtttc acgaacaatc acatgcagac gaataccgaa acgaattttg
    35341 cgtccatgcg cggcagcttt cgcccgcact tgttcgatct tctcttttac cagctctggc
    35401 ggttcgcccc aggtgaggta gagatcaact tgttctgccg ccagctcctg ggcgacatct
    35461 gacgatccgc caaagtaaag cggaggatac ggctgttgaa tcggcgggaa gagcagtttt
    35521 gctccccgca catgaat
//
LOCUS       DAKISA010000037        35107 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.037, whole genome shotgun sequence.
ACCESSION   DAKISA010000037 DAKISA010000000
VERSION     DAKISA010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35107
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.037"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(1..582)
                     /locus_tag="OVW45_001874"
                     /pseudo
     CDS             complement(1..582)
                     /locus_tag="OVW45_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(811..1731)
                     /gene="malM"
                     /locus_tag="OVW45_001875"
     CDS             complement(811..1731)
                     /gene="malM"
                     /locus_tag="OVW45_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCV5526340.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(1883..3223)
                     /gene="lamB"
                     /locus_tag="OVW45_001876"
     CDS             complement(1883..3223)
                     /gene="lamB"
                     /locus_tag="OVW45_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCV5526341.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(3295..4410)
                     /gene="malK"
                     /locus_tag="OVW45_001877"
     CDS             complement(3295..4410)
                     /gene="malK"
                     /locus_tag="OVW45_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCV5526342.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            4775..5965
                     /gene="malE"
                     /locus_tag="OVW45_001878"
     CDS             4775..5965
                     /gene="malE"
                     /locus_tag="OVW45_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCV5526343.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVAVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            6119..7663
                     /gene="malF"
                     /locus_tag="OVW45_001879"
     CDS             6119..7663
                     /gene="malF"
                     /locus_tag="OVW45_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCV5526344.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ECAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGEIGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLLFLLVGALAIVNLKA
                     TRMKFD"
     gene            7678..8568
                     /gene="malG"
                     /locus_tag="OVW45_001880"
     CDS             7678..8568
                     /gene="malG"
                     /locus_tag="OVW45_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCV5526345.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     AIGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            8940..10415
                     /gene="xylE"
                     /locus_tag="OVW45_001881"
     CDS             8940..10415
                     /gene="xylE"
                     /locus_tag="OVW45_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HCV5526346.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(10459..10869)
                     /gene="psiE"
                     /locus_tag="OVW45_001882"
     CDS             complement(10459..10869)
                     /gene="psiE"
                     /locus_tag="OVW45_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCV5526347.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(10995..11138)
                     /locus_tag="OVW45_001883"
     CDS             complement(10995..11138)
                     /locus_tag="OVW45_001883"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5526348.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPAWVRFVLPDAPPGAAYP
                     AYG"
     gene            11139..11417
                     /locus_tag="OVW45_001884"
     CDS             11139..11417
                     /locus_tag="OVW45_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5526349.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(11464..13560)
                     /locus_tag="OVW45_001885"
     CDS             complement(11464..13560)
                     /locus_tag="OVW45_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCV5526350.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTIGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDAYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(13560..14297)
                     /locus_tag="OVW45_001886"
     CDS             complement(13560..14297)
                     /locus_tag="OVW45_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCV5526351.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALEVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(14294..14932)
                     /locus_tag="OVW45_001887"
     CDS             complement(14294..14932)
                     /locus_tag="OVW45_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCV5526352.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDEP
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(15046..15288)
                     /locus_tag="OVW45_001888"
     CDS             complement(15046..15288)
                     /locus_tag="OVW45_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5526353.1"
                     /translation="MKKVLYGIFAISALAATSVWAAPVQVGEAAGAAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTITTTSTQ"
     gene            complement(15750..17399)
                     /gene="pgi"
                     /locus_tag="OVW45_001889"
     CDS             complement(15750..17399)
                     /gene="pgi"
                     /locus_tag="OVW45_001889"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCV5526354.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            17924..19273
                     /gene="lysC"
                     /locus_tag="OVW45_001890"
     CDS             17924..19273
                     /gene="lysC"
                     /locus_tag="OVW45_001890"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCV5526355.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            19525..19797
                     /locus_tag="OVW45_001891"
     CDS             19525..19797
                     /locus_tag="OVW45_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCV5526356.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(19930..20802)
                     /gene="rluF"
                     /locus_tag="OVW45_001892"
     CDS             complement(19930..20802)
                     /gene="rluF"
                     /locus_tag="OVW45_001892"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCV5526357.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            21150..21844
                     /locus_tag="OVW45_001893"
                     /pseudo
     CDS             21150..21844
                     /locus_tag="OVW45_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313031.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            21914..22720
                     /locus_tag="OVW45_001894"
     CDS             21914..22720
                     /locus_tag="OVW45_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCV5526358.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEVHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            22730..23137
                     /locus_tag="OVW45_001895"
     CDS             22730..23137
                     /locus_tag="OVW45_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCV5526359.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            23137..23631
                     /locus_tag="OVW45_001896"
                     /pseudo
     CDS             23137..23631
                     /locus_tag="OVW45_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            23697..24494
                     /locus_tag="OVW45_001897"
     CDS             23697..24494
                     /locus_tag="OVW45_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCV5526360.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            24505..25329
                     /locus_tag="OVW45_001898"
     CDS             24505..25329
                     /locus_tag="OVW45_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCV5526361.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VIMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            25381..26609
                     /gene="sorE"
                     /locus_tag="OVW45_001899"
                     /pseudo
     CDS             25381..26609
                     /gene="sorE"
                     /locus_tag="OVW45_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
     gene            26992..27681
                     /gene="pepE"
                     /locus_tag="OVW45_001900"
     CDS             26992..27681
                     /gene="pepE"
                     /locus_tag="OVW45_001900"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCV5526362.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(27772..29403)
                     /locus_tag="OVW45_001901"
     CDS             complement(27772..29403)
                     /locus_tag="OVW45_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCV5526363.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(29623..33306)
                     /gene="metH"
                     /locus_tag="OVW45_001902"
     CDS             complement(29623..33306)
                     /gene="metH"
                     /locus_tag="OVW45_001902"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCV5526364.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFETLGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNEPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            33506..34330
                     /gene="iclR"
                     /locus_tag="OVW45_001903"
     CDS             33506..34330
                     /gene="iclR"
                     /locus_tag="OVW45_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCV5526365.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            34647..35107
                     /locus_tag="OVW45_001904"
                     /pseudo
     CDS             34647..35107
                     /locus_tag="OVW45_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         8388 a         9092 c         8959 g         8668 t
ORIGIN      
        1 tactatgacc aagttgactc tgcgagaagt caactgtatc aagaatattt tggctaaaat
       61 caacacattc gccactcgtt ttgagaaaaa tcacattgtg taaacgtcga catttaaata
      121 tgacattatc aactgttgcc ttagtaaaat tgcaattctc aatttcttta cattcgaaag
      181 ttacatgttg tagatttgat gataaaaaag acaccttaga aataacgtca cctttaaaat
      241 gacaatttat taaatcaaaa tcagacaggt cgatataccc ctgcgcatct ttatccatca
      301 gaaaattaca agcgcattct attttttcat ttctgtagaa ttttacactt atagtatcat
      361 tactaattga gacttcacgt aacatattta ttgcatgatt ttttcgttcc gttgttttat
      421 atttaaaaat atatcactga tataaataaa gttatctctg tttaaacctg tcaccccatt
      481 gaaattacgt agtaaatcaa gatctactat attatttcct tgaaccacat catgtttcat
      541 caaagagtta ttgccgccag aatttgtgat atgacctatc ataaaacctc tgtaatacag
      601 aaataaaata acctggaaaa gtagcctgag cgtccagaca ctaccggcga aaggaaaaga
      661 atctctgata aggcattgag ataatggaca ttcttagtga agaagaagtg aagtactata
      721 aaaaatcgct catatcccag gaaggaagca taaaaaaagg tgcgccagga gacgcaccag
      781 ttgttgcaaa atcagcactg tggggcgtaa ttaccccttg ccttttacac tgctgataaa
      841 ggtggaacgg gcagatgtcg atcccaggcg ttcagcttca tcaagcagtt ttaacgcctt
      901 atcaacatca cctttcgcga cagcgttttt gatcgcggta ttaaaataac tttccgtgtc
      961 gttgagcatc ggctcgcttt tcttcaccgg caccggagca ggtgcagcca cagctggtgc
     1021 cgccgtgttg cctaccgtaa ccggagctgg agcggaagaa ccaaatagtg gtcctaccaa
     1081 cacgctggag ctggagttcg ttttgacttt cagtttcagt aagccatcgc tggtatgacg
     1141 agcaaccgga tcgggaatat ccgggatcga gttaccgacg cctttggcat aggctttagc
     1201 cgggtcgagc agttgggtcg tctgctggag atctttttcc gtggtaaaga ccagaacata
     1261 aagtttttgc tgccccaacg ccggtgtcag gcgcataacg ccttccagcc gatctgcact
     1321 catcacgcct ggttcctggt aggtgaaata actgctgggg aagaaggctg atggggtcat
     1381 gttctgatca agaatcagca cgttcggcgc aaaaacgctg gtttgtttgt tcacttcgct
     1441 ggtcagcgtc agggtcagtt cgccaatgtt tgccgggacg ctgtacgcag caaccggacc
     1501 actgatgccg ggaacgttca gttgttggcc gccggtcgcc agttgggtgg tctgggtttt
     1561 agattgatcg accggtgtcc aggtgagttg ttgcagcgcg gcagatggaa tggctggcgc
     1621 gtcgctggtg ttttgcggta cgtagttaac atcggcaagg ctaattccag gcgcgcttgc
     1681 cagtaaccct gctgataaac agagggcgat gagactttta ttcattttca ttgttttcac
     1741 ctcaaaatct ggagctcagc ggtagccagg caatagcgcg ctaaacccga taatcagagg
     1801 ggctttcgcc cctttcagat aatgacaacc tgtttttatg ccggatgcgg cgtaaacgcc
     1861 ttatccggcc caggttttgc tattaccacc agatttccat ctgggcaccg aaggtccact
     1921 cgtcgctgtc gccacgaccg aagctgccgc cgttgaaatc agcaggaacg gctttgccgt
     1981 agttcgggtt aaccttgcta tcgccgttgt agtcgtaacc ccatttctca tcccacttgg
     2041 cgtaggttgc gaagacacga atagccgggc gtgaccagat gctgtcgcca gcctgccatt
     2101 gttgtgcgag ggtaattttg tactgattgt tcttgtcgcc ggtgcgctgg gattcgacgt
     2161 tgtcgtagcc gatttccatc acggtgctca tgattggcgt ccacttgtac atcgggcgaa
     2221 taccgacggt ccaccacttg gtgccgttgt cgttatccca gttgatatcc tggtacatac
     2281 ccacgtacat catgtcccag ttgtcgccca tggagatcgc accgtggtcg aggatacgca
     2341 gcatgtgacc gttgttgttg atattgtagg caaatttttc gttatcaaac gcgacgccag
     2401 aaccctgcga cagaccttta ccctgcgagg tcatcgagtc agtagcgtac tgaacaacaa
     2461 acttgttaaa gcccttcagg acactctgag tatgttcagc agtgaataac cagccgtctt
     2521 tcgatgcgcc atcaaccaga cgatagttat cacgcaggtt ggcacgaccg tagtcgacac
     2581 ccagttctaa tgtgccgccc gggttgattt ccatctgcgc taaacgcaca tcgaaaacgt
     2641 cgttcgcggt ttcgttggta tagtcataaa tattgttgct ggcaaaagag gaagaaccac
     2701 cagcttcaga ggagcgggtt gctgccagag agagtttacc gaagccaaca tcgatgtttt
     2761 ccagaccggc accaggacca gaaatatccc agtagtagaa gtcgatcata tgaacgtcat
     2821 gacgttggta gaagcgctta cctgcccaga tggtggaacc tggcagccat tcgatcaggt
     2881 ttttaccctg cacgtttgct tcacggaagg ccggatcggt agcttcccag tcattctgtt
     2941 gcgcgacgga ataggccacg ttagtgtcga aatagaagct cttatcgccc tctttccaca
     3001 cttcctgacc caattttaat tcagcataag tttcacattc gttgccaaga cggtatttac
     3061 tttgagcacc ggtagtctgg aaacactgtt gttcaccgcc gctacctgtc cagccaatac
     3121 cggaacgtgc atagccgtgg aaatcaacag ccattgcctg agcagacatt acgcccgctg
     3181 cgacggcaac cgccagagga agtttgcgca gagtaatcat cattctatct cctgagtcat
     3241 tgcttttctt ttttcacatc acctgtgaca ggctttgtgt gttttgtggg gtgcttaaac
     3301 gcccggctcc ttatgcagtc gacgacatgc agtgccatcc tcacggaaca gatggcaacg
     3361 ctctggcggc aggccgatag cgaatgtggc accttcttct accaacacca cgtcgttctg
     3421 gcggtacacc aggttttgac gaatggaagg gatctggata tggatttggg tttcgttgcc
     3481 gagttgctcg acgacctgaa cttcaccctc aaggatgacg tcagcgatat cactcggcag
     3541 tagatgttcc gggcgaatac ccagcgacat attggctcca acctggacat cacggctttc
     3601 aactggcagc cagacttgct gacgatttgg catcggcagc tccacctgca cttgatcgat
     3661 tgcggtggcg gtcactttta ccggcaggaa gttcatcttt ggcgaaccga taaatccggc
     3721 gacaaaacgg tctgccggat agtggtacag ctccagcggt ttcccaacct gcgccacgcg
     3781 accggcgtcc agcaccacga ttttgtcggc cagcgtcatc gcttcgacct gatcgtgggt
     3841 gacgtaaatc attgtgcggc ccaggcgttt atgcagacgg gagatttcga tacgcatttg
     3901 cacacgcagt gcagcatcga ggttggagag cggttcatcg agcaaaaata cgcttggctc
     3961 ggccaccagc gtacggccaa tcgccacacg ctgacgctga ccaccggaga gcgctttcgg
     4021 tttgcgatcc agcaaatgcg ccagttgcag cacttccgcc acctggttga cgcgttggtt
     4081 aatcacctct ttttttgcgc cagccagttt caggccaaat gacatgtttt ctgctactga
     4141 caggtgggga tagagcgcgt aagactgaaa caccatacca acgccgcgtt ctgctggcgg
     4201 agtgtcattc atccgtttct caccgatgaa caggtcgccg ctggtgatcg tctcaagccc
     4261 ggcaatcatg cgcagtaaag tcgatttacc gcagccagac ggtccgacaa acaccacgaa
     4321 ttcaccttca tggatatcga gattgatatc tttcgatacc acgacctcgc cccaggcttt
     4381 cgttacattt tgcagctgta cgctcgccat gcccttctcc ctttgtaaca acctgtcatc
     4441 gacagcaaca ttcatgatgg gctgactatg cgtcatcagg agatggctta aatcctccac
     4501 cccctggctt ttttatgggg gaggaggcgg gaggatgaga acacggcttc tgtgaactaa
     4561 accgaggtca tgtaaggaat ttcgtgatgt tgcttgcaaa aatcgtggcg attttatgtg
     4621 cgcatctcca cattaccgcc aattctgtaa cagagatcac acaaagcaac ggtggggcgt
     4681 aggggcaagg aggatggaaa gaggttgccg tataaagaaa ctagagtccg tttaggtgtt
     4741 ttcacgagca cttcaccaac aaggaccata gattatgaaa ataaaaacag gtgcacgcat
     4801 cctcgcatta tccgcattaa cgacgatgat gttttccgcc tcggctctcg ccaaaatcga
     4861 agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt
     4921 cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact
     4981 ggaagagaaa ttccctcagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc
     5041 gcacgaccgt tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa
     5101 agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct
     5161 gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc
     5221 gaacccacca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg
     5281 taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc
     5341 tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gatattaaag acgtggccgt
     5401 ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gaccttatta aaaacaaaca
     5461 catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc
     5521 gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg
     5581 tgtaacggta ctgccgacct tcaagggtca accatctaaa ccgttcgttg gcgtgctgag
     5641 cgccggtatt aacgccgcca gtccgaacaa agagctggcg aaagagttcc tcgaaaacta
     5701 tctgctgact gacgaaggtc tggaagcggt caataaagac aaaccgctgg gtgcagtggc
     5761 gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ccatggaaaa
     5821 cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt
     5881 gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga
     5941 cgcgcagact cgtatcacca agtaatgctg tgaaatgccg gatgcggcgt gaacgccttg
     6001 tccggcctac aaaactgaaa cgcatgtagg cctgataaga cgcatcagtg tcgcatcagg
     6061 cagttgttgt cggataaggc gtgaaagcct tatccgtcct ggaatgagga agaaccccat
     6121 ggatgtcatt aaaaagaaac attggtggca aagcgacgcg ctgaaatggt cagtgctagg
     6181 tctgctcggc ctgctggtgg gttaccttgt tgttttaatg tacgcacaag gggaatacct
     6241 gttcgccatt accacgctga tattgagttc agcggggctg tatattttcg ccaatcgtaa
     6301 agcctacgcc tggcgctatg tttacccggg aatggctgga atgggattat tcgtcctctt
     6361 ccctctggtc tgcaccatcg ccattgcctt caccaactac agcagcacta accagctgac
     6421 ttttgaatgt gcgcaggaag tgttgttaga tcgctcctgg caagcaggca aaacctataa
     6481 ctttggtctt tacccggcgg gcgatgagtg gcaactggcg ctcagtgacg gcgaaatcgg
     6541 caaaaattac ctctccgacg cgtttaaatt tggcggcgaa caaaaactgc aactgaaaga
     6601 aacgaccgcc cagcccgaag gcgaacgcgc gaatctacgc gtgattaccc agaatcgtca
     6661 ggcgctgagt gacattaccg ccattctgcc ggatggcaac aaagtgatga tgagctccct
     6721 gcgccagttt tctggcacgc agccgctcta cacactcgac ggtgacggca cgttgacgaa
     6781 taatcagagc ggcgtgaaat atcgtccgaa taaccaaatt ggcttttacc agtccattac
     6841 cgccgacggc aactggggtg atgaaaagct aagccccggt tacaccgtga ctactggctg
     6901 gaaaaacttt acccgcgtct tcaccgacga aggcattcag aaaccattcc tcgccatttt
     6961 cgtctggacc gtggtgttct cgctgatcac tgtcttttta acggtggcgg tcggcatggt
     7021 tctggcatgt ctggtgcagt gggaagcatt gcgcggcaaa gcggtctatc gcgttctgct
     7081 gattctgccc tacgcggtgc catcattcat ttcaatcttg attttcaaag ggttgtttaa
     7141 ccagagcttc ggtgaaatca acatgatgtt gagcgcgctg tttggcgtga agcccgcctg
     7201 gttcagcgat ccgaccaccg cccgcacgat gctaattatc gtcaatacct ggctgggtta
     7261 tccgtacatg atgatcctct gcatgggctt gctgaaagcg attccggacg atttgtatga
     7321 agcctcagca atggatggcg caggtccgtt ccagaacttc tttaagatta cgctgccgct
     7381 gctgattaaa ccgctgacgc cgctgatgat cgccagcttc gcctttaact ttaacaactt
     7441 cgtgctgatt caactgttaa ccaacggcgg cccggatcgt cttggcacga ccacgccagc
     7501 cggttatacc gacctgcttg ttaactacac ctaccgtatc gcttttgaag gcggcggggg
     7561 tcaggacttc ggtctggctg cagcaattgc cacgctgctc ttcctgctgg tgggtgcgct
     7621 ggcgatagtg aacctgaaag ccacgcgaat gaagtttgat taagggagat aacaaaaatg
     7681 gcaatggtcc aaccgaaatc gcaaaaagca cgtttgttta ttactcacct gctactgcta
     7741 ctttttatcg cggcgattat gttcccgctg ctgatggtcg tcgctatctc actgcgccag
     7801 gggaactttg cgatcggcag cctgatcccg gagcaaatct cctgggatca ctggaaactg
     7861 gctttaggtt ttagcgtgga acaggctgat ggtcgcatta cgccaccgcc attcccggta
     7921 ctgctgtggc tatggaactc ggtaaaggtc gccgggattt ccgcgattgg cattgtcgcg
     7981 ctctctacca cctgcgccta cgctttcgcc cgtatgcgct ttccaggcaa agcgacgctg
     8041 ctgaaaggaa tgctgatttt ccagatgttc ccggcagtac tttcactggt cgcgttgtat
     8101 gcgttgtttg atcgtctggg tgagtacatt ccattcattg gcctgaatac tcacggcggc
     8161 gtaattttcg cgtatctggg tgggattgcg ctgcatgtct ggaccatcaa aggctatttc
     8221 gaaaccatcg atagttcgct ggaagaagcg gctgcgctgg atggtgcgac accgtggcag
     8281 gccttccgcc ttgtcctgtt gccgctgtca gtaccgattc tggcggtggt attcatcctg
     8341 tcgtttatcg ctgccattac tgaagttccg gtcgcgtcgc tgttactgcg tgacgtaaac
     8401 agctacaccc tggccgtggg gatgcagcaa tacctcaacc cgcaaaacta cctgtggggt
     8461 gactttgccg ccgctgccgt gatgtctgca ttaccgatca ccatcgtctt cttgctggct
     8521 caacgctggc tggtcaacgg cctgacggca ggtggtgtga aaggttaaag atgttgttct
     8581 gccaatgtta tgccgctgca ccctcaactt acgttatccc aacttgtgac tgttattcgg
     8641 cgctccacgg agcgcctttt tttctttcgt ctgcaatctg aatcgttcgc cggttaatat
     8701 ttccatcata gagcttatta tttttacgtt atttgttttc ccacttacga taattctctt
     8761 tcgtgctctg agtcacggca atagtattgt tttcatcaat ttttgataat tatcacaatt
     8821 aagatcacag aaaagacatt acgtaaacgc attgtaaaaa atgataattg ccttaactgc
     8881 ctgacaattc caacatcaat gcactgataa aagatcagaa tggtctaagg caggtctgaa
     8941 tgaataccca gtataattcc agttatatat tttcgattac cttagtcgct acattaggtg
     9001 gtttattatt tggctacgac accgccgtta tttccggtac tgttgagtca ctcaataccg
     9061 tctttgttgc tccacaaaac ttaagtgaat ccgctgccaa ctccctgtta ggattttgcg
     9121 tagccagcgc tctgattggt tgcatcatcg gcggtgccct cggtggttat tgcagtaacc
     9181 gcttcggccg tcgtgattca cttaagattg ctgctgtcct gttttttatt tctggtgtag
     9241 gttctgcctg gccagaactt ggttttacct ctataaaccc ggacaacaca gtacctattt
     9301 atctggcagg ttatgtcccg gaatttgtta tttatcgtat tattggcggt attggcgttg
     9361 gtttagcctc aatgctttca ccaatgtata ttgcggaact ggctccagct catattcgcg
     9421 gaaaactggt ttcatttaac cagtttgcga ttattttcgg gcaactttta gtttactgcg
     9481 taaactattt tattgcccgt tccggtgatg ccagctggct gaatactgac ggctggcgtt
     9541 atatgtttgc ctcggaatgt atccctgcac tgctgttctt aatgctgctg tataccgtgc
     9601 cagaaagtcc tcgctggctg atgtcacgcg gcaagcaaga acaggcggaa agtatcctgc
     9661 gcaaaattat gggcaacacg cttgcaactc aggcagtaca ggaaattaaa cactccctgg
     9721 atcatggccg caaaaccggt ggtcgtctgc tgatgtttgg cgtgggcgtg attgtcatcg
     9781 gcgttatgct ctccatcttc cagcaatttg tcggcatcaa tgtggtgctg tactacgcgc
     9841 cggaagtgtt caaaacgctg ggggccagca cggatatcgc gctgttgcag accattattg
     9901 tcggagttat caacctcacc ttcaccgttc tggcaattat gacggtggat aaatttggtc
     9961 gtaagccact gcaaattatc ggcgcactcg gaatggcaat cggtatgttt agcctcggta
    10021 ccgcgtttta cactcaggca ccgggtattg tggcgctact gtcgatgctg ttctatgttg
    10081 ctgcctttgc catgtcctgg ggtccggtat gctgggtact gctgtcggaa atcttcccga
    10141 atgctattcg tggtaaagcg ctggcaatcg cggtggcggc ccagtggctg gcgaactact
    10201 tcgtctcctg gaccttcccg atgatggaca aaaactcctg gctggtggcc catttccaca
    10261 acggtttctc ctactggatt tacgggtgta tgggcgttct ggcagcacta tttatgtgga
    10321 aatttgtccc ggaaaccaaa ggtaaaaccc ttgaggagct ggaagccctc tgggaaccgg
    10381 aaacgaagaa aacacaacaa actgctacgc tgtaatcttc ctgtccagca cgccgcgcca
    10441 gttcggcgtg ctttcttttt actcccgctt cagccgtttc gaattacaca gccacagagt
    10501 gatcaccagt aacaggatcg cagccgagta gatcaacaca tccagtggcg atttatgatc
    10561 gacgatgatc aagcgcacaa tggcggtgat cccaatatag acaaagtaac gtagcgggaa
    10621 gtgaaaaccg gactgaaagt acttcacaat cagcgcgata aattcgaaat agagaaagta
    10681 aaccaccagt ccttctacca gctcatattt gctggtttgt tctggcgcga acagcacatc
    10741 tgccagatgc accgtttctt tgccgaggaa gacaaccaaa atcaggccga ggcacagcag
    10801 gccaagattg agtacggtct gcaaaatggt ggagataaac tccacgcgcg gacgagagag
    10861 tgacgtcata gcagtacctc cttcacagtg gcgagcttcc tgtataacgc aaaaatgtga
    10921 cggagatcta tattttttgt gtatgttttg ttcaacctgg atttggagcg cgcctccacc
    10981 aaagccggat atacctaccc gtaggccgga taagccgcgc ctggcggcgc atccggcaag
    11041 acaaaccgca cccaagccgg taattcaaaa atcacccatt caggtgattc ccagccacca
    11101 gcaatgcgcg aaaaatcgac aatgttcctc tggaacaaat gaaacgtaac ctgataaagg
    11161 tggtaaaaat gaagccttat tttgctgctt tgatgttatc agtctctgtt cttcctgctt
    11221 atgctggtcc gctcggtact gctgataaag ccgacctacc gcaaagcaat gtctccagcc
    11281 caatgatggc ccagtcgctc aggcaaccag acctgcaacc gatatcgacg gacaggaaga
    11341 cagaatgttt tcgcctttac acaccagatc gcaaaccggg ggtgaactgc gttcctgatg
    11401 gctcaacagg ccattaagaa tgagggtgcc agtgattgcg aaaactggca cctttcccat
    11461 gacttagcgg aaattgacgc tacggtcgct ggtcatatca tacaactgga acttacgccc
    11521 aagttgctga ccaccgtcac gcgtcagcgg cgtccagcca atcgccgcac ggctgcgtgt
    11581 cgggccagac gagaagagat ccaacggtac cgagacatac acgcctttgg tgaagtcccc
    11641 ttcgccgtac tcctcttttg aaacattcgt gatcgtggca tagccaccca ccaccacgcc
    11701 gctatcaaag cgtttggcga tctccagcgt gccgccttta tcccctgcca gatactgccc
    11761 gacgctggct ttcactaaca cgtcctgggc gaaagatggc gtccagtagg cggtcagatg
    11821 tccggttttc acgctgtagt cggtgaattt catcatatct tttgcactac gccagtcgcg
    11881 ctgtttaacg tagttggcat ccagaccaaa cgcccagttg ctgtccagcg gacgatacaa
    11941 cacttcagcc cccgcaccgc caaacatggt ttcgagataa cctccataga cctgaccgta
    12001 gaagccgttg cccagatgct ggaagtagtt ggcttgcagg ttattcacat aggcatcgtt
    12061 ctgcacatac tcgcgcacat gggtacgaac gcgcggtaag tgcgagtcct gtggcggatt
    12121 ggtgtagtta aacttgtcgt agttgttggc aagatttgca aacaggctgc cgatggtcag
    12181 cagatggtcc gtcagccaca aatctgccgt tcccatcacg cccagctgat acatgtaaaa
    12241 gttttccggg ccaccgaccg actggttcag caccggatcg atatggaaat caaagcgtga
    12301 tttgtcgata taccagccct gctcggtgga ctgcggaacc actggctcga cgcgtttttg
    12361 cgccagcgtc gtttcgtggc ccaacggctc tccggcgaga tgatttttca ggctggcgac
    12421 atcggtttcc gtcgtcacct gcggcatgtt aaggcgattt tccgtaatgc ggatcgtttt
    12481 gatcccatcc ggcagatcgt tcatcacgat ccgattagca cggatgatcc cttcgcgcga
    12541 atcacgatat ttcacctgct cgccggtaac atacagcgta tcgcctttcg cctggatctg
    12601 cggatcggca agtccggcat tgtatttcag caacgttaac tgattcgcca ccaccgagtg
    12661 ctgcaaaatg gcatcctgcg gctgcggttg atattgcggg cgggcgttat cgatgtagga
    12721 cgggcgcaga tcgttaaagt tggtgcgcag cgtgacgcca aacataaagg tgttgccacg
    12781 ttcatagcta aggttaacgt cggcccaatc ggtaacgcga taaatcgccc cgacgttaaa
    12841 cttacttttt tgctccagct tcccggcaaa atcctgctga taattattac cttcatactc
    12901 cagtttcagg cgcagcggtt gccagggcgt ctggtattcc acgccgccaa acagtgaggc
    12961 cgggccgtgg aacatctgac tgccgtcgat agatcccgct tgtttgtagc tgttatcgcg
    13021 atagcaatat ttatcactgg ctgaacagag cggatttttc acattaccgc tggtgcccag
    13081 atatccccag cccaggccga gcgtaaaatc gaacggcccc caggctttgc tggcaacaag
    13141 atattccgca tcaaacagcc ccgtaccgcc gatatcccgc gcaccaaccg ccacttgcgg
    13201 cagccagtaa ctctcttccc acaaacgcag tttgagatcg aaggctttat ctttatacgt
    13261 ttgatcgcca gagaacgctt cgacgctgct gtactgccgg gtgcgcacgt cggtgtaacg
    13321 cagcgttgtt tccagccacg ggaagagttg cactgaagct gagtaataac ggtactgatc
    13381 gttatcgcga tagttcagac tcaactcccc ttcccgtgcc atacgcgcgg tgggagtttg
    13441 taataatcct acgccaccga aatccgactg cgacggacca atgggcgcag gatatgtttc
    13501 gccgtagcaa gctgtgctaa tgcccagcgc cagtaagcta agcagatgtc tttttttcat
    13561 tattggggta tccgctgcgt cagagtctga agaatgtcgg cgttaagggc atcaggcgtc
    13621 tcactccaga cggagtctgc gaggccaaca taaataatgc tgccgggcat cggctctacg
    13681 tgacgcttgt tccagtaagc caccggcgct ttttgcgtgc gtccgtccgg gtaaacgacc
    13741 cacgcgtagc tgcgatccgc accgctgagc aggctctggt cagagagata gctcgccacg
    13801 tcgcgacctg gagtgaatgg ctgattgcca ggatggctga taagcccgaa caacgtgacc
    13861 gtggacggcg gtggtccgac ccacagcgtg tagttgccct gcaacggcgg gttaccgcgt
    13921 tcggcaacgc ggacgatatc gggatcaaga ttgatttttt gcctgcctgt cacttccaat
    13981 gcctggattt gctggcgtaa ggcgttaatg gcggcggcat cgtcggtgct ggaatctgcg
    14041 ccctgttctg ccagccgtgt cagcaacgcc tgctgctgac gcaatgctgc cgccgttgcc
    14101 agctcttcgc taatcaccgc accaggccac cagctgtttg ccagtcgagg ttgccctacc
    14161 agatcgatta aatgctctgc gcccgttagc gttttggcct cactgctgcc attgctgaac
    14221 accttgacgg ttcctgccgc gaagaccgat gacgctccca cactcaaaag caacgcgaca
    14281 atagtttgtt taatcattgt tttgccgcct tgatgagagt ggttttcacc gggaaataat
    14341 ccgcgccgag atactgttcc gactggcgaa tttgcccttc gctgtcgatc cagtagcggt
    14401 tatgccagcg cgcctggtcg gtggagactt cttcgtccag cacgcgaacc aggggttcat
    14461 cgctgccgac cttgacggta tcggtgccat cccatttgaa gactgagcgt gcggtggcgt
    14521 agcgtacctg ctggtactcg gtccagccca tcgtgcgcgt ccatgttgca ccatcaacaa
    14581 tttgtgcggg tttaatcagc gggtcggcgg caaggttatt cacttcaatc aggttgtcgc
    14641 cgccaagcaa agtcttcacc agacgaccat gttgtgtgac aagagtggct tgatcctgag
    14701 tgacccattt ttgttgtccg tcttcagcga aggccagcac cacaaacaat tgcggcccgc
    14761 cattaagctg catgtactgg ctggcgtagg gcatattttg aatatcatcg tccgtcagct
    14821 gtacgcctgg cgtgccgaac agactgtccc acagtgaatt gcccagctct ttagtcgtgg
    14881 ctgaacaggc ctgtaataac aggcagataa gaatgagtgc aggtcgcttc acgactcttc
    14941 tccagagggg cagaataacc acaccggagt gtggttatgg ttaatccctt catatttcaa
    15001 gccacagatg caatgactcg aattattttg gggatacata ctttattact gggtactggt
    15061 ggttgtgatt gttgtggtcc cggtattaga accatcaccg ccaccggttg ccgcgagcgc
    15121 gacacccacc gccgagctta cggtgctgac gctggtcgcg gaggaactcc ccgccgaaac
    15181 cgacgttgct gccgcccctg ccgcttcgcc cacctgtaca ggtgcagccc acacagaagt
    15241 cgccgcaagc gcagatatgg caaaaatgcc atacagaact tttttcataa caatttccct
    15301 tcattgaatg aatggagatt tacccaaaaa catttcgggc gggatcgagt atacacaact
    15361 aaagcatgcg aattacggcg cggggaatta gcgatggtgt tttaggataa ttggaatgaa
    15421 tatcaaatat aagtttcaac ttatatttaa ataacaataa aaatcataaa cttaaaataa
    15481 aaacaaaagt aattaatctg cgtattctcc gaaataaaca acgattaaaa atctatttta
    15541 ggattaacct cacggtatga tttccgttaa atcacagaca aggggattta tctgataaaa
    15601 caatgccgac ttattgtcgg cattgttttt aaggttattt aactcctcat actatgcatt
    15661 aatgtcgata ttggcatcag gctccggttg ccggatgcgg cgtaaacgcc ttatccggcc
    15721 tacatatcgg catcaggcac gcatgatgat taaccgcgcc acgctttata gcggttaatc
    15781 agaccattgg tcgagctatc gtggctgctg atttctttgt catctttcag ctctggcaga
    15841 atacggttcg ccagttgttt acccaactcc acgccccact ggtcgaaggt aaagatgttc
    15901 aggatcacgc cctgagtaaa gattttgtgc tcatacagcg caatcaacgc acccaggctg
    15961 aacggagtga tttcgcgcag caggatggag ttggtcgggc ggttaccttc gaacactttg
    16021 aacggcacca cgtagtcaag cgttgccgga tctttaccct gatcgcgata ttcctgctca
    16081 accacttcgc gggatttacc aaacgccagc gcttcggtct gggcgaagaa gttagacagc
    16141 agtttctggt ggtgatcaga gagcgggtta tgggtgatag ccggggcgat gaaatcgcac
    16201 ggtaccattt tggttccctg gtggatcagc tggtagaacg cgtgctgacc gttagtgcct
    16261 ggttcacccc agataatcgg gccagtctgg taatccacaa cgttaccgtt acggtcaaca
    16321 tacttaccgt tggactccat attgccctgc tggaagtacg ccgcgaaacg gtgcatatac
    16381 tggtcatacg gcagaatcgc ttcagtttcc gcaccaaaga aattgttgta ccagatgcca
    16441 atcagcgcca gcaggacagg caggtttttc tcggcaggcg tggtggagaa atgcttgtcc
    16501 atcgcgtgtg cgccagaaag cagttcaacg aagttatcaa agccgatgga gagaacaatc
    16561 gacaggccaa tcgctgacca caaagagtaa cggccgccga cccagtccca gaactcgaac
    16621 atgttggcag tatcaatacc aaactcgcca acggctttgg cattggtgga aagcgccgca
    16681 aagtgttttg caacgtgttt ttcatcacct gccgctttca ggaaccagtc acgcgcgcta
    16741 tgggcgttgg tcatagtttc ctgagtggtg aaggttttag atgctaccag gaacagcgtg
    16801 gtttccgggt ttactttttt cagcacttca gcgatgtgag tcccatcgac gttagaaaca
    16861 aagtgcatgt tcaggtgatt tttgtacgga cgcagtgctt cggtcaccat gtacgggccg
    16921 aggtcagaac caccgatccc gatgttcact acgtcagtga ttgctttgcc ggtataaccc
    16981 ttccactcac cggagataat cgcttccgag aaggttttca tcttctccag caccgcgttg
    17041 acttccggca ttacgtcttt gccatcaacc agaatcgggg tattgctacg gttacgcagc
    17101 gctacgtgca gcacggcgcg gttttcagtg cggttgatct tctcgccaga gaacatcgac
    17161 ttaatcgcgc ccgccagatc acactctttc gccagatcct gtaatttcgc cagcgtctct
    17221 tcagtgatgc ggtttttgga gtaatccacc agcatctgat cgtcgaatgt tgcggagaac
    17281 ttagaaaaac gatcgccgtc tttagcaaaa agatcggcga tcgtaacgtc tttcatttca
    17341 tcgaagtgtt tctgtagtgc ctgccaggca gcggtctgcg ttggattgat gtttttcatt
    17401 agcaatactc ttctgatttt gagaattgtg actttggaag attgtagcgc cagtcacaga
    17461 aaaatgtgat ggttttagtg ccgttagcgt aatgttgagt gtaaaccctt agcgcagtga
    17521 agcatttatt agctgaacta ctgaccgcta ggagtggatg aaaaatccgc atgaccccgt
    17581 cgttgacaac cgccccgctc accctttatt tataaatgta ctacctgcgc tagcgcaggc
    17641 cagaagaggc gcgttgccca agtaacggtg ttggaggagc cagtcctgtg ataacacctg
    17701 agggggtgca tcgccgaggt gattgaacgg ctggccacgt tcatcatcgg ctacaggggc
    17761 tgaatcccct gggttgtcac cagaagcgtt cgcagtcggg cgtttcgcaa gtggtggagc
    17821 acttctgggt gaaaatagta gcgaagtatc gctctgcgcc cacccgtctt ccgctcttcc
    17881 cttgtgccaa ggctgaaaat ggatcccctg acacgaggta gttatgtctg aaattgttgt
    17941 ctccaaattt ggcggtacca gcgtagctga ttttgacgcc atgaaccgca gcgctgatat
    18001 tgtgctttct gatgccaacg tgcgtttagt agtcctctcg gcttctgctg gtatcactaa
    18061 tctgctggtc gctttagcgg aagggctgga acctggcgag cgattcgaaa aactcgacgc
    18121 tattcgcaac atccagtttg ccattctgga acgtctgcgt tacccgaacg ttatccgtga
    18181 agagattgaa cgtctgctgg agaacattac tgttctggca gaagcggcgg cgctggcaac
    18241 gtctccggcg ctgacagatg agctggtcag ccacggcgag ctgatgtcga ccctgctgtt
    18301 tgtcgaaatc ctgcgcgaac gcgatgttca ggcacagtgg tttgatgtac gtaaagtgat
    18361 gcgtaccaac gaccgatttg gtcgtgcaga gccagatgta gccgcgctgg cggaactggc
    18421 cgcgctgcag ctgctcccac gcctcaatga aggcttagtg atcacccagg gatttatcgg
    18481 tagcgaaaat aaaggtcgta caacgacgct tggccgtgga ggcagcgatt atacggcagc
    18541 cttgctggcg gaggctttac acgcatctcg tgttgatatc tggaccgacg tcccgggcat
    18601 ctacaccacc gatccacgcg tagtttccgc agcaaaacgc attgatgaaa tcgcgtttgc
    18661 cgaagcggca gagatggcaa cttttggtgc aaaagtactg catccggcaa cgttgctacc
    18721 cgcagtacgc agcgatatcc cggtctttgt cggctccagc aaagacccac gcgcaggtgg
    18781 tacgctggtg tgcaataaaa ctgaaaatcc gccgctgttc cgcgcgctgg cgcttcgtcg
    18841 caatcagact ctgctcactt tgcacagcct gaatatgctg cattctcgcg gtttcctcgc
    18901 ggaagttttc ggcatcctcg cgcggcataa tatttcggta gacttaatca ccacgtcaga
    18961 agtgagcgtg gcattaaccc ttgataccac cggttcaacc tccactggcg atacgttgct
    19021 gacgcaatct ctgctgatgg agctttccgc actgtgccgg gtggaagtag aagaaggtct
    19081 ggcgctggtc gcgttgattg gcaatgacct gtcaaaagcc tgcggcgttg gcaaagaggt
    19141 attcggcgta ctggaaccgt tcaacattcg catgatttgt tacggcgcat ccagccataa
    19201 cctgtgcttc ctggtgcccg gcgaagatgc cgagcaggtg gtgcaaaaac tgcatagtaa
    19261 tttgtttgag taaatactgt atggcctgga agctatattt cgggccgtat tgattttctt
    19321 gtcactatgc ttatcaataa acgagcctgt actctgttaa ccaacgtctt tatcgagaat
    19381 aattgccttt aattttttta tctgcatctc taattaatta tcgaaagaga taaatagtta
    19441 agagaaggca aaatgaatat tatcagttct gctcgcaaac gaattcccgt acactgaact
    19501 ctctggctca ctgagggcac catcatggca ctcccccgta ttacccaaaa agagatgact
    19561 gaacgcgagc agcgtgaact taaaacactg ctggatcggg cgcggattgc gcatggtcgc
    19621 gtactgacca actccgaaac caacagcatt aagaaagagt acatcgataa attaatggtc
    19681 gagcgtgagg ctgaagcgaa aaaagcccgc caattgaaga aaaagcaggc ttataaaccg
    19741 gatcctgaag cgtcatttag ctggtcagca aatacgtcga cgcgcggcag gcgctaatga
    19801 acaatcaacg cctgagattt tcactcgtca gtgtgaacac aattttcccc tcaccctaac
    19861 ccttttcccg aaggggcgag gggactgtcc gagcactttt gggccgtttt catcagtctg
    19921 aggcgttaat cagcgcccct tctttttacg ccccggcgag gtaaaacgct taccgttgga
    19981 cgccgggcga ccgccttttt ccgccgtttt ttccatctta acgactggac gtttgatgcc
    20041 cgctgttttc ggtttggcct tcgctttggg cttcgcctcg gaagaggaat tttcaatgag
    20101 cttaaagaga tcgataagct cgtcgtcggt taaatcgcgc cattctccca gcggaatgcc
    20161 gcttaagctg acgttcataa tgcgcgtacg ttccagcttt ttcacttcat agccgaaatg
    20221 ctcgcacatg cgccggatct gacggttcaa cccctgcacc agggtaatgc ggaagacaaa
    20281 cggtgcttct tttttgactt tgcacttttt cgtcaccgtc ccgaggatcg gcactcccgc
    20341 ccccatacca cgaataaatt catcggtaat cggtttatcg accgtcacca gatactcttt
    20401 ctcatgatcg ttacccgcac ggaggatctt attcaccaga tcgccgtgat tggtgaggaa
    20461 aatcagcccc tgggagtctt tatccaggcg gccaatcggg aacacgcgtt tgctgtggtt
    20521 aacgaaatcg acaatgttat cgcgctcgcc atcttcggtg gtgctgacaa taccgacggg
    20581 cttgttcagg gcgataagta ccaaatcttc ggcttcccga ggctcaatca actgaccgtt
    20641 tactttcaca atgtcgccgg gtttcacctg atcaccaatg gtggctcgct tgccattaag
    20701 gaacacattg ccttgctcga tatagcgatc cgcttcgcgg cgtgagcaaa ttccgctttc
    20761 gctgatgtat ttatttaaac ggactgatga gtcgggcagc ataaattctc ctgtaaaacg
    20821 cggaatatac cgtacctctt agcggggaaa aacagggtta tgcggcaacg tcgaaattaa
    20881 atgtgatctg ccacgccttt tatcacaaaa gtgtctccgg aagtacgatg caaatttgcc
    20941 tggcaaaata aggataatca acatattacg aatcgctgca cacctgtgct gatgactact
    21001 acgcagagaa acaaatgtgc aagagaaagc gctatttctc cccatgcaaa gagtgaaaaa
    21061 tcgctttgaa aggccctcat aatggggtta tatcagatca agcggtgaga aagcgtattt
    21121 gcacaaatgt gcagaaccag gaggcggcaa tggaaaacag tgacgatatc cgtttgattg
    21181 tgaagattgc ccaactctat tacgaacagg atatgacgca ggcgcaaatc gcgcgcgaac
    21241 tgggtattta ccgcaccacc atcagccgct tgcttaaacg aggccgcgat cagggaattg
    21301 tcaccatcgc catcaactat gactacaacg aaaatctctg gctggagcag caactgaagc
    21361 aaaagtttgg cttgaaagac gttgtggtgg tgtcgggaaa tgatgaggat gaagagactc
    21421 aactggcgat gatggggtta cacggcgcgc aactgctgga tcgcttgctg gagcctggcg
    21481 atattgtcgg tttttcctag ggtcgcgcgg tgagcgcact ggttgaaaac ttgccgcaag
    21541 cggggcaata accccttaat tcgtaatggg atcatgcagt ctcagcactt taaaaccatc
    21601 tctgcctact gggataatct ggatgtcgcc ctggtgggaa ttggctcacc ggccattcgc
    21661 gacggcgcta actggcatgc gttttatggt ggtgaagaga gtgacgacct gaaagcaggc
    21721 gcggtattcc attggcattg ccatgagtga agaaaaatac agcggaattg ttggtgcact
    21781 gcgtggaaaa tatattaatt gtctggtaac gaatagcagc acagctgaac tattactgaa
    21841 ataaaaatct tcccgatgtt taccgtaata aaaattacgg cgggtcccta ttatctaaaa
    21901 tcaggagtct gttatgcaaa cgtggttaaa tttgcaggat aaaatcatta tcgtcaccgg
    21961 cggcgcatcc ggtattggtc tggcgattgt agaggaatta ttagcacaag gcgcaaacgt
    22021 acagatggta gatattcacg gtggcgatgg tcaatatgaa gttcataaag gctatcagtt
    22081 ttggccgacc gatatttcca gcgccaaaga ggtaaatcat acggtagcgg aaattatcca
    22141 gcgttttggt cgcatcgacg gtctggtcaa taacgccggg gtcaatttcc cgcgtctgct
    22201 ggtcgatgag aaagcgcctg ccgggcagta tgaactcaac gaagctgcat tcgaaaaaat
    22261 ggtcaatatc aaccaaaaag gcgtttttct gatgtcgcag gcggtggcgc gccagatggt
    22321 caaacaacat gatggcgtga ttgtgaatgt ttcctcagaa agtggactgg aaggctcaga
    22381 aggccaaagc tgttacgccg ctaccaaagc cgcgctcaat agcttcacgc gctcctggtc
    22441 gaaagagctg ggtaaacacg gtatccgtgt ggtcggtatc gcgccgggga ttctggaaaa
    22501 aaccgggcta cgcacgccgg aatatgaaga ggcgctggcg tggacgcgca atatcaccgt
    22561 cgagcagctg cgtgaaggct ataccaaaaa cgccattcct attgggcgcg ccggaagatt
    22621 agcagaagtc gctgattttg tttgttatct gctgtctgaa cgcgccagct acatcaccgg
    22681 agtaaccact aacattgcgg gcggcaaaac gcgcggataa ggaggcttta tggtcaacgc
    22741 cattttttgt gcccacggca aattagcttg cgccatgctg gaatcggttc agatggtgta
    22801 cggcgacgcc aatgtcgagg cggttgaatt tgtaccaggc gagaacgccg gagacatcgt
    22861 agcaaagctg gaaaagttag taagcattca caatcaagat gagtggctga ttgccgtgga
    22921 tttacagtgc ggcagcccgt ggaatgcggc agcgatgctt gcgatgcgca atcctcgtct
    22981 gcgggtaatc agcggccttt ccctgccgct ggcgctcgaa ctggttgata accaggacag
    23041 catgaatgtt gatgaactgt gtgaacacct gacgcaaatc gcaaagcaaa cctgcgttgt
    23101 ctggaaacaa ctggcaacgg cagaggaaga tttctgatga acattacgct cgcccgcatt
    23161 gatgaccgtc tgatccacgg ccaggtcact accgtctggt cgaaagtggc taacgcccag
    23221 cgcattatta tctgcaatga cgaagtttat aacgatgaag tgcgccggac attattgcgt
    23281 caggctgcgc caccgggaat gaaggttaac gtggtcaata ttgaaaaagc cgtcgcggtt
    23341 tatcacaacc cgcaatatta ggacgaaacc gttttttatc ttttcactcg cccacaggat
    23401 gccttagcga tggttcgcca gggggtaaaa ataggcaccc tgaatattgg cggcatggcc
    23461 tggcggcccg gtaaaaaaca gttaaccaaa gcggtttcat tagatgatga tgatattaac
    23521 gcatttcatg agttaaataa tcttggcgtc ataatggatt tacgcgtcgt agcgtcagat
    23581 ccctcaatca atattatcga caaaataaac gaacagttaa tcgcaaatta aataatagcg
    23641 cctgtgtaca tatgctttaa caggcaggga tgacgacact taaaaggtga caaatcatgg
    23701 aaataagtac cctacaaatt attgctatat ttcttttttc ctgtattgcc ggaatgggca
    23761 gcgtgctgga tgaatttcag actcatcgcc cgttaattgc ctgtacggtg attggtttaa
    23821 ttctcggtga tttaaaaacc ggaataatac tcggtggtac gctggaattg atagctctcg
    23881 gctggatgaa cgtcggcgcg gcgcaatctc cggattctgc actcgccagc ataatctccg
    23941 ccattctggt tatcgttggt cagcaaagca tcgccaccgg aatcgccatc gcgttgcctg
    24001 tggctgcggc aggccaagtg ctgacggtgt ttgcccgtac catcaccgta gtgttccagc
    24061 acgcggcgga taaagcagcg gaagaggcgc ggtttcgcac tctcgatatt ctgcatgtct
    24121 ccgcgcttgg cgtgcaggcg ctgcgcgttg ctattccggc actgattgtc tcactgttcg
    24181 tcagcgccga tatggtcagc aatatgttaa acgccattcc cgaatttgtg acccgtggac
    24241 tgcaaattgc tggcggtttt atcgtggtgg tcggttacgc catggtgcta cgtatgatgg
    24301 gcgtgaaata tttgatgcct ttctttttcc tcggtttcct cgcaggtggc tacctcgatc
    24361 tcagcctgct ggcgttcggt ggcgtcggcg tgatcatggc cctggtctac atccagctaa
    24421 atccacagtg gcgtaaagct gaacctcaac tccaggccac cgcctccacc gcccttgacc
    24481 aacttgacga ttaacggagc catcatggaa cagagaaaaa ttacacgcag cgatctggtg
    24541 agcatgtttc tgcgctccaa cctgcaacag gcgtccttta actttgaacg tattcacgga
    24601 ctgggctttt gctacgacat gatccccgcc atcaagcgac tttacccatt aaaagaggat
    24661 caggttgcgg cgctcaggcg acacctggtg ttcttcaata ccacgccagc cgtatgtggc
    24721 ccggtcatcg gcgtcactgc cgccatggaa gaggcgcggg ccaacggcgc ggaaattgat
    24781 gacggtacca ttaacggcat caaagtcggt ctgatgggac cattggcagg agttggcgat
    24841 ccactggtct ggggaacgct gcgcccgatt accgctgcgc tcggcgcatc tctggcactt
    24901 tctggcaaca tcctcggccc gctgctgttt ttctttattt tcaatgcggt gcgtctggcg
    24961 atgaagtggt atggcctaca gctcggcttt cgcaaagggg tgaatatcgt cagcgatatg
    25021 ggcgggaatg tgctgcaaaa actcaccgaa ggcgcgtcga ttctcggact gtttgtgatg
    25081 ggcgtgctgg tcaccaaatg gacgtcaatc aacgtaccgt tggtggtttc acaaacgcat
    25141 gccgccgatg gctccaccgt catcatgacc gtgcagaaca ttctcgacca actttgccct
    25201 ggtttgctgg cgctcggtct gacgctactg atggttcgtc tgctcaacaa aaaaattaac
    25261 ccggtatggc tgattttcgc cctgtttggc ttagggatta tcggcaatgc gctgggcttc
    25321 ctgtcctgat tcttcgcccc ggcacgactg ccggggccat cgctcaacat gaggtggttt
    25381 atgaaaacaa cagctctgcg tctttatggt aaacgtgatt tacgcctgga aacctttgac
    25441 cttcctgaaa tgcaggagga tgaaatcctc gcgacggtgg tcactgacag cctgtgcctc
    25501 tcttcctgga aagaggcaac ctgggtgaaa accataaaaa agtacccgac gatgtggcga
    25561 ccaacccaat catcatcggc cacgagtttt gcggcgatat tctggccgtg ggtaaaaagt
    25621 ggcagcacaa attccagccg ggtcagcgtt atgtgattca ggccaacctg aaactccctg
    25681 accgcccgga ctgccccggc tactccttcc cgtgggtggg cggcgaagcc acgcatgtgg
    25741 ttattcccaa cgaggtcatg gaacaagatt gcctgctggc atacgacggc gaaacctatt
    25801 ttgaaggctc gctggttgaa ccgctttcct gcgtgattgg cgcgttcaac gccaactatc
    25861 atcttcagga aggtagttat aaccacacga tggggattcg cccgcaaggg cgcacgctga
    25921 tcctcggcgg caccggacca atgggattgt tggcgattga ttatgcgctg catggaccag
    25981 ttaacccgtc gctgctcgtc attaccgata ccgacaacga caaattgagt tatgcgcgca
    26041 agcactatcc atcagaaccg caaacactga ttcattatct caatgccacc gatgcagcat
    26101 ttgatacgtt aatggcgctg agtggcggtc acggcttcga tgatattttc gtctttgtgc
    26161 ctaatgaagg actggtgact ctcgcctctt ccttgctggc gacagatggt tgcctgaatt
    26221 tcttcgccgg accgcaggat aaacatttca gcgcgccaat taatttctac gatgtgcatt
    26281 atgcatttac ccactacgtg ggcacgtcag gcggcaatac cgacgacatg cgcgcggcgg
    26341 tcaaactgat tgaagagaaa aaagtgcagg ccgcaaaagt ggtaacacat attcttgggc
    26401 tgaatgccgc gggcgaaacc acgcttgaat tgcctgccgt cggcggcggc aaaaagctgg
    26461 tgtataccgg gaaatacctg ccgctgacgt cactcacgca gattcaggat caagcactgg
    26521 cggcgattct ggcgcgtcat caggggatct ggtccggtga ggcggagcaa tatctgctca
    26581 ctcatgcaga ggcaatttcc catgattaat cgcgctactt aactctgcat gtcgctggct
    26641 ggtcgccgcg gcaacttcgc gacctgatgt gcggctgatt gctgaagcgg cccaatgtgc
    26701 acgagaagga taaaaaaaca tcagcgcctt aaggggcgct caaattggtg gcaaagtaca
    26761 cattgtttat gccggatgcg gaataaagcc ttattcggcc tacataatct cgaaaattca
    26821 atatattgca gttatctggt aggcctggta agcgtagcgc atcaggcaat gctgtgtttg
    26881 tcatcagtct cagcgcccgg tgcaatacgt gacatcgtga gccatgtcag taagtggtta
    26941 aattcgcagt ggtagcatgc cttccgtgat ttcttactaa ccggagaagt aatggaactg
    27001 cttttattga gtaactcgac gctgccgggt aaagcctggc tggaacatgc actgccgcta
    27061 attgctgaac agttgcaggg tcgccgctca gcggtgttta tccctttcgc tggcgtaacg
    27121 cagacctggg atgattacac agcgaaaacg gctgcggttc tcgctccgct gggtgtttct
    27181 gtcaccggta ttcatagcgt tgtcgatccc gttgccgcga ttgaaaatgc tgagatcgtg
    27241 attgtcgggg gcgggaatac tttccagttg ctgaaacagt gccgcgagcg cgggctgctg
    27301 gcaccaatta ctgacgtggt taaacgtggt gcgctgtata ttggctggag cgcaggcgct
    27361 aaccttgctt gcccaaccat tcgtaccacc aacgatatgc cgattgtcga tccgcaaggt
    27421 ttcgatgcgc taaatctgtt ccctctgcaa atcaacccgc acttcaccaa cgcgctgccg
    27481 gaaggccata aaggtgaaac ccgtgagcag cgtattcgcg aactgctggt cgtcgcgcca
    27541 gaactgacga ttattggtct accggaaggt aactggatca cggtgagtaa aggtcacgct
    27601 acgctgggtg gcccgaacac cacttatgtg tttaaggccg gtgaagaagc ggtgccgctg
    27661 gaagcgggtc accgttttta agtgaattgc cggatgcatg atccggcatc ttgttcatag
    27721 tgcctgatgc gacgcttgac gcgtcttatc aggcctacag gattcacaat attaatactc
    27781 gtcccgtcct tcatcttcat ccggctgttc cagcacactg taagccaccg aacaaaacag
    27841 cgagttcaga cgctgcatat ctcccagtaa gcctaaatgt agcgaactgg tttcaatgct
    27901 ctgcacgttt tgctgatgca ggcgatcgac gtgggcgtgg gaatagcggc gattaagaat
    27961 gcgaaagcga tgcttgctgc ggcgcaaacg acgagcgctg gtgacatcgc cagagaagaa
    28021 caccgacatt gccagcttta aattactgag caattgctca tagagcgcat ccagttcctt
    28081 caacccatca agcgaaaatg cccgccgtgc tgccagcgat ttatcagcaa tttcgctgcc
    28141 catgcgctcg acgatatcgg aagcctgttc aaggttgagc gacatttcaa taatctccgc
    28201 ccagcgacgc gactcttctt ctgccagttc ctcttttggc attcgcgcca gatacagctt
    28261 aatggcggtg tagagaacgt tgatatcatc tgccagctta cgcagctctt tctcctgccg
    28321 tggctcgccg tgcatcactt tattcagccc ttccatcatc tgttccatgg cgtcgccaat
    28381 gcgcagggtt tcgcgcgcgg cgttcgccag agcaagcgtg ggcgtatcca gcgcgctgac
    28441 atccagatgt ttaggccgta gctgggtatc cagttccggt tcatcgcgaa taatcgtttt
    28501 gcaaaaccgt gccatcgggt caacaaatgg cagcatgacc aggcaacgta caaggttgta
    28561 gaagacgtgg aaatagatca ccagttccgc ttttggcaat gacaacttcc ccattgtctc
    28621 tgccagcaaa tggacaaacg gcaggataat caggctaccc accagcttaa acagcaaact
    28681 acccagcgcg acacggcggg ctgcggcatt ggcggcactg ttgttgagca tcgccagcag
    28741 gccggaaccg aggttagcac caatcaccag acagagcgcc acggggaagg agataatgcc
    28801 tgcggcggtc agagtcgcgg tcagcagtac agcagcaagg ctggagtagc tgataatggc
    28861 gaacatcgcg ccaatcagcg catccagcag aatatcgccg gtcagcgagg caaagatcac
    28921 ctgaacgccg tttgcctggg tgatcggcgt tacggcctgc acaatcaact ccagcgccag
    28981 caaaatcagc ccaagaccaa taccgacgcg gcccagttgc ccggcgcgtg actgtttgcg
    29041 tccgaggaaa aaaatcacgc cgataaaaat aagtaacggt gacagccagg ataagtcgaa
    29101 ggtgagaata cgcgccatta gcgccgtccc gacatctgca cccagcacaa tcaccagagc
    29161 cggtgcgagg gctaccagat cctgagcgac gaacgaggtc accagcatgg tggtggcatt
    29221 actgctctgt accagtgcgg taacgccgat ccccgcgcaa aaggcgagcg gctttttttc
    29281 gacgctccgg ctaaggacag tacgcaaacg cgcgccgaag acgcgcatta cgccggttcg
    29341 aacaatatga gtcccccaga ccagcagggc gacggcagaa agcaggtgaa gcagcgttaa
    29401 cacgagttca ggttctcctt atcgttatat gttcctgaag caattgaatg accaaaggac
    29461 cggaaacacg ctgactggca tctttactgt gccttttgac attcgttttt ccggctaaga
    29521 ttattccagc ctgtttccag tataagtgtt tcatattaat aaagagacag ggcactcatt
    29581 gagcgccctg tttgttgtaa ggaaaagtga cagatttgtg aatcagtccg cgtcataccc
    29641 cagattcggt gccagccagc gctcaacatc ggagacgctc atacctttac ggcgggcata
    29701 gtcttcaacc tgatcgcgct gaatttgcgc caccgcgtag tacttgctgt ccgggtggct
    29761 gaaataccaa cccgaaaccg atgcaccagg ccacatggcg aaagattctg tgagtttcat
    29821 gccagtatgt ttttccactt ccagcagctc ccagatggtg gctttttccg tatgttccgg
    29881 gcaggccggg tagcccggtg ccggacggat gccctggtag ttttcgcgga tcagttcttc
    29941 gttgctgagg ttctcgttcg gcgcatagcc ccagtagact ttacgcacac gctcatggag
    30001 atactccgca aaggcttcgg ctaaacggtc ggcaagcgct ttcaccatga ttttgttgta
    30061 atcatcgtgc tgcgcttcaa aggcatcagc cagtgcgtcc tcttccagcc cgccagtcac
    30121 ggcaaatgcg ccgatgtaat ctgctttacc agaaagcttc ggcgcaacga agtcagcgag
    30181 acagtagtta gcgaatcccg ttttttcggt ctgttgacgc agatggtggc tgacgttgat
    30241 cacatgggtg cgcgtttcgt cacggtagat ttcaatgtca tcgcccacac ggtttgccgg
    30301 gaacaagccc accacgccac gcggattcag cgttttctcg gcgcttaatt tatccagcat
    30361 gtcgttggcg tctttaaaca gccgctgcgc ctcaacgccc accacttcat cttccagaat
    30421 gcgcggatac ttcccggcca gcgaccaggt cataaagaac ggtgtccagt cgatgtaatt
    30481 acgcagagtt tcgatgctgg cttcaacttc ctgcacgccg agacggtgcg ccaccggcgg
    30541 cgtgtaagcc tgccagtcaa aagcgaagtc gttatcgcgc gccgcttcca gcgtgaccgg
    30601 tggtgtgcgc ggtttcttgc gcccgtgctg aatacgtacg gtttcatact ccttgcgggt
    30661 acgagcgaca aaatcatcgc gctgagtatc ggaaagcagc gccgccacca caccaacggt
    30721 gcgcgaggca ttctgcacat acaccgtcgg gccgctgtag ttctgctcaa ttttcaccgc
    30781 cgtgtgcgct tttgaggtcg tcgcgccacc gatcagtaac ggaatggtga agccctgacg
    30841 ctccatctct ttcgccacgt taaccatctc gtccagcgac ggcgtgataa gccccgaaag
    30901 tccaatcaga tcagcattca cttctttagc ggtacggaga attttttccg caggcaccat
    30961 aacgccgaga tcgacaattt cgtagttgtt acattgcagc accacaccaa cgatattttt
    31021 accgatgtcg tggacgtcgc ccttcacggt ggcgatcacc atcttgccgt tggttttgcc
    31081 ctgctctttg ctggcttcaa taaacggttc gaggtaggcc actgcctgtt tcatgacgcg
    31141 cgccgatttg accacctgtg gcaggaacat tttcccctcg ccaaacagat cgccgaccac
    31201 gttcatgccg tccatcaacg ggccttcaat cacttcaatc gggcgcgtag cctgctggcg
    31261 ggcttcttcg gtatcctgct cgataaactc agtaatgcct ttgaccagcg agtattccag
    31321 acgtttattc acttcccacg agcgccactc cgcctgctgg gcgttagcgg tgtcgtcggt
    31381 tttgctgccg cgatatttct cggcaagctc cagtaaacgt tcagtgccat catcgcgacg
    31441 attaagaatc acatcttcca ccgcgtcgcg cagttcagcg ggcaggtcgt cgtaaatcgc
    31501 cagttgcccg gcgttgacga tccccatatc catgccattg cgaatagcgt agtagaggaa
    31561 caccgcgtga atggcttcgc gcaccggttc gttgccacgg aatgagaaag aaacgttaga
    31621 tacgccgccg gaaatcagcg cgtgcggcag ttcgcgtttg atgtcttcgc aggcgccgat
    31681 aaagtcctgc gcatagttgt tgtgctcttc aatgccggtg gcgaccgcga agatgttcgg
    31741 gtcgaagatg atatcttctg gcgggaagcc aacctcttcg gtgaggattt tgtacgcccg
    31801 acggcaaatc tcgattttcc gtgcgcgggt atcggcctgt ccctgttcgt caaaggccat
    31861 taccaccacc gctgcaccgt aacgacgcaa cagtttcgca tgatggataa aggcgtcgac
    31921 gccctctttc attgagatag agttaacaat gcctttgccc tgaatacact tcagcccctt
    31981 ttcgatgacc tcccattttg aggagtcgat cataatcggc acgcgagcga tatccggttc
    32041 accggcaatc agattgagaa aacgcaccat cgccgcttcg gcgtcgagca tcccttcatc
    32101 catattgata tcgataatct gcgcgccgtt ttccacctgc tggcgggcca catccagtgc
    32161 ctcgctgtat ttctcttctt tgatcaggcg cttgaactta gcggaaccgg tgacgttggt
    32221 gcgttcaccc acgttcacaa acaggctatc ttcgccaatg ttcagcggct ccaggccgga
    32281 caaacggcag gctacgggga tttccggcag tttgcgcggc gctaatcctt ctactgcacg
    32341 actcatcgct gcaatatgtt gtggcgtggt gccacagcag ccgccgacga tattgagaaa
    32401 gcccgcttcc gcccattcac gtatttgttt tgccatcgtg tcggcgtcga gatcgtactc
    32461 accaaaggcg ttgggtagcc cggcgttcgg gtgcgcggtg acgtagcatt ccgcaatccg
    32521 tgacagctcc tgcacgtact ggcgcagttc atcgggcccc agcgcacagt tcaggccaaa
    32581 ggtcagcgct tcggcgtggc gcaatgagtt gtaaaatgct tcggtggtct gcccggagag
    32641 cgtgcgcccg gaggcgtcgg tgatggtgcc agagatcata atcggcagct caacgcccag
    32701 cgtttcaaac tccgttttta ccgcaaatac cgccgctttg gcgttaaggg tgtcgaaaac
    32761 ggtttcaatc aggatcagat ccgcaccacc ttccaccagc gctttggtgg actctcgata
    32821 agccgccacc agcccgtcaa aagtgatatt acgaaatgcc ggatcgttga cgtccggaga
    32881 aatagacgcc gtgcggttgg ttgggccgag aacaccggca acgtagcgcg gtttctccgg
    32941 cgtgcgcgcg gtccactcgt cagcacaggc gcgcgccagt ttcgccgccg caaagttgat
    33001 ttccgccgac agggattcca tctggtaatc cgccatcgca atggtcgtgg agttgaaggt
    33061 gttggtttcg atgatatccg cgcccgcttc aaagtaggcg ttgtggatag cggcgatcac
    33121 ttccggttta ctgagtacca gcaggtcgtt gttgcctttg aggtcgcatg gccagtcggc
    33181 aaagcgttca ccacgaaaat cggcttcgtt cagtcgatag ctctggatca tggtgcccat
    33241 accgccgtcc agcaccagaa tacgttcatt taactgcgca cgcagttgtt ccactttgct
    33301 gctcacactt gctcccgaca cgctcaaccc agatttaata aaaattcaac aaaccatact
    33361 ggcataaacg catctgtggt aaaagcgacc accacgcaac atgagatttg ttcaacatta
    33421 actcatcgga tcagttcagc aactattgca ttaactaaca ataaaaatga aaatgatttc
    33481 cacgatacag aaaaaggaga ctgtcatggt cgcacccatt cccgcgaaac gcggcagaaa
    33541 acccgccgtt gccaccgcac cagcgactgg acaggttcag tctttaacgc gtggcctgaa
    33601 attactggag tggattgccg aatccaatgg cagtgtggca ctcacggaac tggcgcaaca
    33661 agccgggtta cccaattcca cgacccaccg cctgctaacc acgatgcaac agcagggttt
    33721 cgtgcgtcag gttggcgaac tgggacactg ggcaatcggc gcacatgcct ttatggtcgg
    33781 cagcagcttt ctccagagcc gtaatttgtt agcgattgtt caccctatcc tgcgcaatct
    33841 aatggaagag tctggcgaaa cggtcaatat ggcggtgctt gatcaaagcg atcacgaagc
    33901 gattattatc gatcaggtac agtgtactca cctgatgcgt atgtccgcac ctatcggcgg
    33961 taaattgccg atgcacgctt ccggtgcagg taaagccttt ttagcccaac tgagcgaaga
    34021 acaggtgacg aagctgctgc accgcaaagg gttacatgcc tatacccacg caacgctggt
    34081 gtctcctgtg catttaaaag aagatctcgc ccaaacgcgc aaacggggct attcatttga
    34141 cgatgaggaa catgcactgg ggctacgttg ccttgcagcg tgtattttcg atgagcaccg
    34201 tgaaccgttt gccgcaattt ctatttccgg accgatttca cgtattaccg atgaccgcgt
    34261 gaccgagttt ggcgcgatgg tgattaaagc ggcgaaggaa gtgacgctgg cgtacggtgg
    34321 aatgcgctga ctttttctgg cgggcagagg caatattctg cccatcatac ctgagtggca
    34381 atagaataag ggtgtctgtt aatcgcattg acgccaaaat aacttaatgt catacacttc
    34441 acacactttt attaactcag cattattttt aaacatcaaa ccacttaatt attaacaaaa
    34501 tcgcaagatc tcattccacc gaaactttta cggctgcatt atgagattga gcgatttatg
    34561 atatttcttt agttgtaatc atattaaatt ttttcactgt tctgatagtt aaaatttaag
    34621 acatcaataa acaatgagat atttaaatga ttactcgtat tcctcgtagt tctttctctg
    34681 caaatattaa taatacagcc cagacaaatg aacaccaaac cctgagtgaa ttgttttata
    34741 aagaactcga ggataaattt tctggcaagg agctggcgac gcctctatta aaaagcttct
    34801 cagagaactg tagacaaaat ggtcgtcata tctttagcaa caaggatttt gtcattaaat
    34861 tttccacgtc cgtcttacaa gctgataaga aagaaattac gataattaat aaaacgaaaa
    34921 cacgacactc actcaaacca ttgctccaat atttgaagaa tacctaatgg aaattttacc
    34981 tcaacgctca gacactcttg ataaacaaga attaaaccta aaatcagata gaaaagaaaa
    35041 agaattccca agaattaaac ttaatggtca atgttatttt ccggggcgac cccaaaaccg
    35101 tatagta
//
LOCUS       DAKISA010000038        34981 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.038, whole genome shotgun sequence.
ACCESSION   DAKISA010000038 DAKISA010000000
VERSION     DAKISA010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34981
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.038"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..815)
                     /locus_tag="OVW45_001905"
     CDS             complement(<1..815)
                     /locus_tag="OVW45_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCV5526366.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="OVW45_001906"
                     /pseudo
     CDS             907..993
                     /locus_tag="OVW45_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1397..1873)
                     /locus_tag="OVW45_001907"
     CDS             complement(1397..1873)
                     /locus_tag="OVW45_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCV5526367.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1932..3221)
                     /gene="bioA"
                     /locus_tag="OVW45_001908"
     CDS             complement(1932..3221)
                     /gene="bioA"
                     /locus_tag="OVW45_001908"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCV5526368.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKICDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVADIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3308..4348
                     /gene="bioB"
                     /locus_tag="OVW45_001909"
     CDS             3308..4348
                     /gene="bioB"
                     /locus_tag="OVW45_001909"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCV5526369.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            4345..5499
                     /gene="bioF"
                     /locus_tag="OVW45_001910"
     CDS             4345..5499
                     /gene="bioF"
                     /locus_tag="OVW45_001910"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCV5526370.1"
                     /translation="MSWQEKINAALDARRTADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEKFGVGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5486..6241
                     /gene="bioC"
                     /locus_tag="OVW45_001911"
     CDS             5486..6241
                     /gene="bioC"
                     /locus_tag="OVW45_001911"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCV5526371.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6234..6911
                     /gene="bioD"
                     /locus_tag="OVW45_001912"
     CDS             6234..6911
                     /gene="bioD"
                     /locus_tag="OVW45_001912"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCV5526372.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7490..9511
                     /gene="uvrB"
                     /locus_tag="OVW45_001913"
     CDS             7490..9511
                     /gene="uvrB"
                     /locus_tag="OVW45_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCV5526373.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9703..10611)
                     /gene="yvcK"
                     /locus_tag="OVW45_001914"
     CDS             complement(9703..10611)
                     /gene="yvcK"
                     /locus_tag="OVW45_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCV5526374.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10876..11020
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            11008..11997
                     /gene="moaA"
                     /locus_tag="OVW45_001915"
     CDS             11008..11997
                     /gene="moaA"
                     /locus_tag="OVW45_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCV5526375.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEVGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12019..12531
                     /gene="moaB"
                     /locus_tag="OVW45_001916"
     CDS             12019..12531
                     /gene="moaB"
                     /locus_tag="OVW45_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCV5526376.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12534..13019
                     /gene="moaC"
                     /locus_tag="OVW45_001917"
     CDS             12534..13019
                     /gene="moaC"
                     /locus_tag="OVW45_001917"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCV5526377.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIVGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13012..13257
                     /gene="moaD"
                     /locus_tag="OVW45_001918"
     CDS             13012..13257
                     /gene="moaD"
                     /locus_tag="OVW45_001918"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCV5526378.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13259..13711
                     /gene="moaE"
                     /locus_tag="OVW45_001919"
     CDS             13259..13711
                     /gene="moaE"
                     /locus_tag="OVW45_001919"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCV5526379.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            13848..14552
                     /locus_tag="OVW45_001920"
     CDS             13848..14552
                     /locus_tag="OVW45_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCV5526380.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14757..15470
                     /locus_tag="OVW45_001921"
                     /pseudo
     CDS             14757..15470
                     /locus_tag="OVW45_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
     gene            complement(15535..16246)
                     /locus_tag="OVW45_001922"
                     /pseudo
     CDS             complement(15535..16246)
                     /locus_tag="OVW45_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
     gene            complement(16246..17487)
                     /gene="clsB"
                     /locus_tag="OVW45_001923"
     CDS             complement(16246..17487)
                     /gene="clsB"
                     /locus_tag="OVW45_001923"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCV5526381.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGDAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(17484..18245)
                     /locus_tag="OVW45_001924"
     CDS             complement(17484..18245)
                     /locus_tag="OVW45_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCV5526382.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18378..18788
                     /locus_tag="OVW45_001925"
     CDS             18378..18788
                     /locus_tag="OVW45_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCV5526383.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18750..19856)
                     /locus_tag="OVW45_001926"
     CDS             complement(18750..19856)
                     /locus_tag="OVW45_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCV5526384.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(19867..21000)
                     /locus_tag="OVW45_001927"
     CDS             complement(19867..21000)
                     /locus_tag="OVW45_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCV5526385.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(20993..22729)
                     /locus_tag="OVW45_001928"
     CDS             complement(20993..22729)
                     /locus_tag="OVW45_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCV5526386.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22722..23717)
                     /gene="hlyD"
                     /locus_tag="OVW45_001929"
     CDS             complement(22722..23717)
                     /gene="hlyD"
                     /locus_tag="OVW45_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCV5526387.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERYLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(23720..24391)
                     /gene="cecR"
                     /locus_tag="OVW45_001930"
     CDS             complement(23720..24391)
                     /gene="cecR"
                     /locus_tag="OVW45_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCV5526388.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGSDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24620..25984
                     /gene="rhlE"
                     /locus_tag="OVW45_001931"
     CDS             24620..25984
                     /gene="rhlE"
                     /locus_tag="OVW45_001931"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HCV5526389.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(26216..26698)
                     /gene="ybiA"
                     /locus_tag="OVW45_001932"
     CDS             complement(26216..26698)
                     /gene="ybiA"
                     /locus_tag="OVW45_001932"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCV5526390.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26818..28968
                     /gene="dinG"
                     /locus_tag="OVW45_001933"
     CDS             26818..28968
                     /gene="dinG"
                     /locus_tag="OVW45_001933"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCV5526391.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRVPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTANNQEEQKRCAKLK
                     GDLDTYRWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            28996..29958
                     /gene="ybiB"
                     /locus_tag="OVW45_001934"
     CDS             28996..29958
                     /gene="ybiB"
                     /locus_tag="OVW45_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCV5526392.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGAALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMVCCLVATGEAATISDGLARVNQAF"
     gene            30099..31184
                     /gene="hcxB"
                     /locus_tag="OVW45_001935"
     CDS             30099..31184
                     /gene="hcxB"
                     /locus_tag="OVW45_001935"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCV5526393.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            complement(31413..31673)
                     /locus_tag="OVW45_001936"
     CDS             complement(31413..31673)
                     /locus_tag="OVW45_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCV5526394.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPITASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31938..32204)
                     /gene="ybiI"
                     /locus_tag="OVW45_001937"
     CDS             complement(31938..32204)
                     /gene="ybiI"
                     /locus_tag="OVW45_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCV5526395.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(32278..32955)
                     /gene="ybiX"
                     /locus_tag="OVW45_001938"
     CDS             complement(32278..32955)
                     /gene="ybiX"
                     /locus_tag="OVW45_001938"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCV5526396.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSQHCV
                     PPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(32997..34981)
                     /locus_tag="OVW45_001939"
                     /pseudo
     CDS             complement(32997..34981)
                     /locus_tag="OVW45_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8271 a         8979 c         9489 g         8242 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatatg ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccactgca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcgtt cagcttttta tactaacttg agcgaaacgg
     1321 gaaggtaaaa agacaaaaag ttgtttttaa tacctttaag tgataccaga tggcattgcg
     1381 ccatctggca gagtgattaa ctaaacatcg cagtaatcga ggcgcttgcc agagagtgga
     1441 aatgaacgtt aaacccgacc atcgcgccgc tggcaccttc atcgacatca atacgttcta
     1501 tatccagcgc gtgaacggta aaaatgtagc gatgagtttc gcctttcggc ggtgctgcgc
     1561 catcgtaccc ggttttacca aagtcggtac gcgtctgcaa aacgccgtct ggcattgcta
     1621 ccagaccaga gccaaaccct tgcggtaata cgcgggtatc agcgggtaag ttaacaacta
     1681 cccagtgcca ccagccggag ccggttggcg catccgggtc gtagcaggtg acaacaaaac
     1741 ttttcgttcc cgcaggaaca tcatcccacg ccagatgcgg tgaaatatta tcgccatcgt
     1801 aacccatgcc gttaaagaca tgacgatgcg gcaatttatc gccatcgcgc agatcgttac
     1861 tgatgagttt catgaaccct cctttcttgt ttgcagaaag tgtagccaga aaccctcacg
     1921 cggacttctc gttattggca aaaaaatgtt tcatcctgta ccgcgcggtt aaccgctgcg
     1981 gtcagacgct gcaactgttg cgggagaata atatagggcg gcatcaggta aatcagtttg
     2041 ccaaaaggcc ggatccagac accctgttcg acaaagaatt tttgcagcgc cgccatattc
     2101 accggatgag tggtttcgac cacgccaatg gcccccagta cgcgcacatc ggcaaccatt
     2161 tcggcatcac gggcgggggc aagttgctcg cgcagctgta cttcaatatc cgccacctgt
     2221 tgctgccagt cgccagattc gagaatcgcc aggctggcgt ttgctgccgc gcaggccagc
     2281 ggattgccca taaaagttgg cccatgcata aagcaaccgg cttcaccgtt actgatggtt
     2341 tctgcaacct cgcgcgtggt gagtgtggcg gaaagggtca ttgtgccgcc ggttaaggct
     2401 ttaccgaggc acaaaatgtc cggcgcgatt tctgcatgtt cacaggcaaa cagtttcccg
     2461 gtacgaccaa atccagtggc gatctcgtcg gcaatcagca agataccttc gcgatcgcat
     2521 attttgcgga ttcgttttaa ccattccgga tggtacatgc gcatcccgcc tgcgccctgg
     2581 acaatcggct caatgatcac cgccgcgatt tcatgacgat gcgccgccat caggcgggca
     2641 aagcccacca tatcgcgctc atcccattcg ccatccatac ggctttgtgg ggcgggagca
     2701 aacaagtttt ctggcagata gcctttccac aggctgtgca ttgagttgtc cggatcgcac
     2761 accgacatcg cgccaaaggt atcgccatga taaccattgc ggaaggtcag aaaacgctgg
     2821 cgcgcttcgc ctttggcttg ccagtactgc aacgccattt tcatcgccac ttccaccgct
     2881 acggaaccgg agtccgcgag aaaaacgcac tccagcggtt gcggcgtcat cgccaccagt
     2941 ttgcggcaca gctcaatggc tggcgcatgg gtgataccgc caaacatcac atgcgacatg
     3001 gcatcaattt gcgacttcat cgccgcatta agctgcgggt gattgtagcc gtggatcgcc
     3061 gcccaccagg acgacatacc gtcaaccagg cgtctgccgt cagacaaaat cagctcgcaa
     3121 ccttcggcgc tcaccaccgg ataaaccggc agcggggagg tcatggatgt gtatgggtgc
     3181 cagatatggc gttggtcaaa ggcaagatcg tccgttgtca taatcgactt gtaaaccaaa
     3241 ttgaaaagat ttaggtttac aagtctacac cgaattaaca acaaaaaaca cgttttggag
     3301 aagccccatg gctcaccgcc cacgctggac cttgtcgcaa gtcacagaat tatttgaaaa
     3361 accgttgctg gatctgctgt ttgaagcgca gcaggtccat cgccagcatt tcgatcctcg
     3421 tcaggtccag gtcagcacgt tgctgtcgat taagaccgga gcttgtccgg aagattgcaa
     3481 atactgcccg caaagctcac gctacaaaac cgggctggaa gccgagcggt tgatggaagt
     3541 tgaacaggtg ctggagtcgg cgcgcaaagc gaaagcggca ggatcgacgc gcttctgtat
     3601 gggcgcggcg tggaagaatc cccacgaacg cgatatgccg tacctggaac aaatggtgca
     3661 gggggtaaaa gcgatggggc tggaggcgtg tatgacgctg ggcacgttga gtgaatctca
     3721 ggcgcagcgc ctcgcgaacg ccgggctgga ttattacaac cacaacctgg acacctcgcc
     3781 ggagttttac ggcaatatca tcaccacccg cacttatcag gaacgcctcg atacgctgga
     3841 aaaagtgcgc gatgccggga tcaaagtctg ttctggcggc attgttggct taggcgaaac
     3901 ggtaaaagat cgcgccggat tattgctgca actggcaaac ctgccgacgc cgccggaaag
     3961 cgtaccaatc aacatgctgg tgaaggtgaa aggcacgcca ctggcggata acgatgatgt
     4021 cgatgccttt gattttattc gcactattgc ggtagcgcgg atcatgatgc cgacctctta
     4081 cgtgcgcctt tctgccggac gcgagcagat gaatgaacag actcaggcga tgtgctttat
     4141 ggctggcgca aactcgattt tctacggttg caaactgctg accacgccga atccggaaga
     4201 agataaagac ctgcaactgt tccgcaaact ggggctaaat ccgcagcaaa ctgccgtgct
     4261 ggcaggggat aacgaacaac agcaacgtct tgaacaggcg ctgatgaccc cggacaccga
     4321 cgaatattac aacgcggcag cattatgagc tggcaggaga aaatcaacgc ggcgctcgat
     4381 gcacggcgta ctgccgatgc cctgcgtcgc cgttatccgg tggcgcaagg agccggacgc
     4441 tggctggtgg cggatgatcg ccagtatctg aacttttcca gtaacgatta tttaggttta
     4501 agccatcatc cgcaaattat ccgcgcctgg cagcagggcg cagaaaagtt tggtgtaggt
     4561 agcggcggtt ccgggcatgt cagcggttat agcgtggtgc atcaggcgct ggaagaagaa
     4621 ctggccgagt ggcttggcta ttcgcgggcg ctgctgttta tctctggttt tgccgcgaat
     4681 caggcagtca ttgccgcgat gatggcgaaa gaggaccgta ttgttgccga ccggcttagc
     4741 catgcctcct tgctggaggc tgccagttta agcccgtcgc agcttcgccg ttttactcat
     4801 aacgatgtcg ctcatctggc gcgactgctt gcttccccct gtccggggca gcaactggtg
     4861 gtgacagaag gcgtgttcag catggacggc gatagcgcgc cactggcgga aatccagcag
     4921 gtaacgcaac agcacaatgg ctggttgatg gtcgatgatg cccacggcac gggcgttatc
     4981 ggggagcagg ggcgcggcag ctgctggctg caaaaggtaa aaccagaatt gctggtggtg
     5041 acttttggca aaggatttgg cgtcagtggg gctgcggtgc tttgctccag tacggtggcg
     5101 gattatctgc tgcaattcgc ccgccacctt atctacagca ccagtatgcc gcccgctcag
     5161 gcgcaggcat tacgtgcgtc gctggcggtc attcgccgtg atgaggggga tgcacggcgc
     5221 gaaaaactgg tgtcactcat tgcgcgtttt cgtgccggag tacaggattt gccgtttacg
     5281 cttgctgatt catgcagcgc catccagcca ttgatcgtcg gtgataacag ccgtgcgtta
     5341 caactggcag aaaaactgcg ccagcaaggc tgctgggtca cggcgattcg cccgccaacc
     5401 gtacccgctg gtactgcgcg actgcgctta acgctaaccg cagcgcatga aatgcaggat
     5461 atcgaccgtc tgctggaggt gctgcatggc aacggttaat aaacaagcca ttgcagcggc
     5521 atttggtcgg gcagccgcgc actatgagca acacgcagag ttacagcgcc ggagtgctga
     5581 cgtcttactg gcaatgctcc cacagcgtaa atacacccac gtactggacg cggggtgtgg
     5641 gccaggctgg atgagccgcc actggcgaga gcgtcacgca caggtgacgg cgttagatct
     5701 ctcaccgcca atgcttgtgc aagcccgcca gaaagatgct gcagaccatt atctggcggg
     5761 agatatcgaa tccctgccgt tagcgactgc gacgttcgat cttgcatgga gcaatctcgc
     5821 ggtgcagtgg tgcggtaatt tatccacggc actccgcgag ctgtatcggg tggtgcgccc
     5881 cggaggcgtg gtggcgttta ccacgctggt gcagggatcg ttacccgaac tgcatcaggc
     5941 gtggcaggcg gtggacgagc gtccgcatgc taatcgcttt ttaccgccag atgaaatcga
     6001 acagtcgctg aacggcgtgc attatcaaca tcatattcag cccatcacac tatggtttga
     6061 tgatgcgctc agtgccatgc gttcgctgaa aggcatcggt gctacgcatc ttcatgaagg
     6121 gcgcgacccg cgaatattaa cgcgttcgca attgcagcga ttgcaactgg cctggccgca
     6181 acaacagggg cgatatcctc tgacgtatca tctttttttg ggagtgattg ctcgtgagta
     6241 aacgttattt tgtcaccgga acggataccg aagtggggaa aaccgtcgcc agttgtgcac
     6301 ttttacaagc cgcgaaggca gtaggttatc ggacggcagg ttataaaccg gtcgcctctg
     6361 gcagcgaaaa gaccccggaa ggcttacgta atagtgacgc gctggcgtta cagcacaaca
     6421 gcagcctgca gctggattac gcaacagtaa atccttacac cttcgcagaa cccacttcgc
     6481 cgcacatcat cagcgcgcaa gagggcaggc cgatagaatc attggtaatg agcgccggat
     6541 tacgcgcgct tgaacaacag gctgactggg tgttagtgga aggtgctggc ggctggttta
     6601 cgccgctttc tgacactttc acttttgcag attgggtaac acaggaacaa ctgccggtga
     6661 tactggtagt tggtgtgaaa ctcggctgta ttaatcacgc gatgttgact gcgcaggcaa
     6721 tacaacacgc cggactgact ctggcgggtt gggtggcgaa cgatgttacg cctccgggaa
     6781 aacgtcacgc tgaatatatg accacgctca cccgcatgat tcccgcgccg ctgctgggag
     6841 agatcccctg gcttgcagaa aatccagaaa atgcggcaac cggaaagtac ataaaccttg
     6901 ccttgttgta gccattctgt atttggttaa attgcgagcg agatcgcgtc ttcgattgac
     6961 tgcaatttaa ccaattaaat tctaaaataa tcacgaaaaa aattttactt ccgcctcatg
     7021 cggcgaatgt gggaattgcc caggcggcgg gggatagggg ctggagacag ttatccacta
     7081 ttcctgtgga taaccatgtg tattagagtt agaaaacacg aggcaagcga gagaatacgc
     7141 ggcttgcacg cgaattggcg ttaaagacgg ctcaaagaaa tatcttttat tttttaactg
     7201 gttagataaa tgcaatggca gtcactgaac aggcatctct tgccataaaa ctgtcatcac
     7261 tcatcttgac aaatgttaaa aaagccgttg ctttggggat aacccggtaa ggccggagtt
     7321 ttatctcgcc acagagtaaa ttttgctcat gattgacagc ggagtttacg ctgtatcaga
     7381 aatattatgg tgatgaactg tttttttatc cagtataatt tgttggcata attaagtacg
     7441 acgagtaaaa ttacatacct gcccgcccaa ctccttcagg tagcgactca tgagtaaacc
     7501 gttcaaactg aattccgctt ttaaaccttc tggcgatcag ccagaggcga ttcgacgtct
     7561 cgaagagggg ctggaagatg gcctggcgca ccagacgtta cttggcgtga ctggctcagg
     7621 gaaaaccttc accattgcca atgtcattgc tgaccttcag cgcccaacca tggtacttgc
     7681 gcccaacaaa acgctggcgg cccagctgta tggcgaaatg aaagagttct tcccggaaaa
     7741 cgcggtggag tatttcgtct cctactacga ctactatcag ccggaagcct atgtaccgag
     7801 ttccgacact ttcattgaga aagatgcctc ggttaacgaa catattgagc agatgcgttt
     7861 atccgccacc aaagcgatgc tggagcggcg tgatgtggtt gtagtggcct ctgtttccgc
     7921 gatttatggt ctgggcgatc ctgatttata tctcaagatg atgctccatc tcacggtcgg
     7981 tatgattatc gatcagcgcg cgattctgcg ccgactggcg gagctgcaat acgctcgtaa
     8041 tgatcaagca ttccagcgtg gtactttccg cgttcgtggc gaggtgatag atatcttccc
     8101 ggcagaatcg gatgacattg cacttcgcgt ggaactgttt gacgaggaag tggaacgatt
     8161 gtcgttattt gacccgctga ccgggcagat tgtttccact attccacgtt ttaccatcta
     8221 cccgaaaacg cactacgtca caccgcgcga gcgcatcgta caggcgatgg aggagatcaa
     8281 agaagaactg gccgccagac gcaaagtgct gttggaaaac aacaaactgc tggaagagca
     8341 gcggctgacc cagcgtaccc agtttgatct ggagatgatg aacgagctgg gctactgttc
     8401 ggggattgaa aactactcgc gcttcctctc cggtcgtgga ccgggtgagc caccgccgac
     8461 gctgtttgat tacctgcctg ccgatgggct gctggtggtc gatgaatctc acgtcaccat
     8521 tccacaaatt ggcggcatgt atcgcggtga ccgggcgcgt aaagagacac tggtggagta
     8581 cggcttccgc ctgccatcag cgctggataa ccgtccgctt aagtttgaag agttcgaagc
     8641 attagcgccg caaaccatct atgtttcggc gacgccgggt aattacgagc tggaaaaatc
     8701 cggcggcgat gtggtggatc aggtggtgcg tccaaccgga ttgcttgacc cgattatcga
     8761 agtgcggccg gtggcgacac aggttgatga tcttctttcg gagattcgtc agcgagcggc
     8821 aattaacgaa cgcgtactgg tcaccacact gaccaagcgg atggcggaag atcttaccga
     8881 atatctcgaa gaacatggcg agcgcgtgcg ttatcttcat tcagatatcg acaccgtcga
     8941 acgtatggag attatccgcg acttgcgtct gggtgagttc gacgtgctgg tagggatcaa
     9001 cttactgcgc gaaggtctgg atatgccgga agtgtcgctg gtggcgatcc tcgacgctga
     9061 caaagaaggc ttcctgcgtt ccgaacgttc gttgatccag actattggtc gtgcggcacg
     9121 taacgttaac ggtaaagcga ttctctacgg cgataagatc accccatcaa tggcgaaagc
     9181 gattggcgaa accgaacgtc gccgtgagaa acagcagaag tacaacgagg aacacggaat
     9241 tacgccgcaa ggcttgaaca agaaagtggt cgatatcctg gcgctggggc agaacattgc
     9301 caaaaccaaa gcgaagggca gaggaaaatc gcgcccgatt gttgagccgg ataatgtgcc
     9361 gatggatatg tcgcctaaag cgttgcaaca gaaaatccat gagctggaag ggttaatgat
     9421 gcaacacgcg cagaatctgg agttcgaaga agcggcacaa attcgtgacc agttgcatca
     9481 gctgcgtgag ctgtttatcg cggcttcgtg acaggataac gaagaagaca gatgacaaaa
     9541 gcaaaattgc ctgatgtgct acgcttatca ggcctacatg atctctgcaa tacattgaat
     9601 tagcgcggtt tgtaggccgg ataaggcgtt cacgccgcat ccggcataaa caacgcgcac
     9661 tttgtcttca ataggaagcc ggaatttttc ttcggatttc cgttaaccta aagcctgtaa
     9721 cgccttttcc agcgcgttat gtaacaactg gcggtcatga cgatacggaa tatcgctggc
     9781 ctccagtacc tcctgaatca caatccgctc tttcaccgcc gagacatcca cttttggccc
     9841 gacgatgacc gcatcaatga cctttttacc aacatactgc tccataattg ccagcttgct
     9901 ttccagcttc aaattagccg caggtaaact caactcacgc cccagattgc cgatatagac
     9961 catcggcgct ggcgtgcggc gtaatgcctg ggcgatttcc ttcagcagca gaattggcat
    10021 caggctggta taaaaactgc caggcccaat aatgatgaga tccgcttcat tgatagcgtg
    10081 aaccgcctca cgcgttgcgg gtacattcgg cgttaataat aactcttgaa tcggcgtagt
    10141 taactggtcg atattgacct cgccgtaaac ttcatgcccc tgatcgtcaa tcgccatcag
    10201 atcgacggga tgctctgaca ttggaatcaa atgcgtatcc actttcagca gattacgaat
    10261 taaattgatg gcttccagag gccgcacgct aaggtgatcc agcgccttta acatcaagtt
    10321 tccgagatta tgaccggaaa gttcgccatt gccgccaaaa cggtattcaa acatcgcgga
    10381 ggcgacgctc ggttccgtta tcagttggtt gaggcagttg cgcatatcgc cccaggcaat
    10441 gccgccttct gaacggcgaa tacgccccgt cgagccacca ttatccgtgg tggtgacgat
    10501 acccgttaaa cgagaaccca aagacgaaag tgatgagaga acgcgtccca gtccatgccc
    10561 tccgccgaga gcaacgacac gatcaagatc agccagcgta cgattgcgca tatagtttcc
    10621 tggagtcaga ttatccgcgc tacagtagcg caaattgcgg ggaaacagca attaacctgc
    10681 caatattgag gatacaaact attttcttct acctctaaag gacgatgcac gctatgcctc
    10741 cctgatgatg tatatcaaag taaaactgcc attttccctt attctgtagc gaaatagcac
    10801 gatcatgacg ctatatacat gattacatag cgaaagtgtg gatggtaaaa atctcattta
    10861 cacgctagta tcggcttaac cactaaacac tctagcctct gcacctgggt caactgatac
    10921 ggtgctttgg ccgtgacaat gctcgtaaag attgccacca gggcgaagga agaaatgact
    10981 tcgcctcccg tatctggaaa ggtgtacatg gcttcacaac tgactgatgc atttgcgcgt
    11041 aagttttact acttgcgcct gtcgattacc gatgtgtgta actttcgttg cacctactgc
    11101 ctgccggatg gctacaaacc gagcggcgtc accaataaag gctttcttac cgtcgatgaa
    11161 attcgccggg ttacgcgcgc cttcgccagt ctgggcaccg aaaaagtgcg cctgacagga
    11221 ggagagccgt ctttacgccg cgactttacc gatatcatcg ccgctgtgcg ggaaaacgac
    11281 gctatccgcc agattgcggt caccaccaat ggttaccgtc tggaacgcga tgtggcgaac
    11341 tggcgcgatg cgggacttac tggcattaac gtcagtgtcg acagtctgga cgcccgccag
    11401 tttcacgcta ttaccgggca ggataaattc aaccaggtca tggcagggat cgatgctgca
    11461 tttgaggtcg gttttgagaa ggtcaaagtc aataccgtgc tgatgcgtga tgttaatcat
    11521 caccagctcg acacctttct gaactggatc cagcatcgcc ctatccagct gcgtttcatc
    11581 gaactgatgg aaacgggcga gggcagtgag ctcttccgta agcatcacat ctctggtcag
    11641 gttctgcgtg acgagctact gcgtcgcggc tggatccacc aattacgtca acgcagcgac
    11701 ggtcccgcgc aagtcttttg ccatccagat tacgccggag agattggcct tatcatgccg
    11761 tatgaaaaag acttctgcgc cacttgcaac cgcctgcgcg tttcctccat tggtaaactc
    11821 catctctgcc tgtttggtga aggcggcgtt aacctgcgcg atctgctgga agacgatacc
    11881 cagcaacagg cgctggaagc gcgtatttca gcggcgctgc gggagaagaa acagacccat
    11941 ttcctgcatc aaaacaacac cggtattacg caaaacttat cgtacattgg cggctaaaac
    12001 gtcaaaagga gagatcagat gagtcaggta agcactgaat ttatcccgac ccgtattgct
    12061 attcttacgg tttctaatcg tcgcggtgaa gaagacgata cctccggtca ctatttgcgc
    12121 gattcggcgc aagaagcggg ccatcacgtt gtcgataaag ccattgtgaa agaaaaccgc
    12181 tacgctattc gcgctcaggt atctgcgtgg atcgccagcg acgatgtaca agtggtgttg
    12241 attacggggg ggactggcct gacggaaggt gatcaggctc ccgaagcatt gctgccgttg
    12301 ttcgaccgtg aagttgaagg ttttggtgaa gtgttccgga tgttgtcgtt tgaagagatt
    12361 ggcacttcca cgttgcaatc tcgtgcggta gcgggcgtcg ccaacaaaac gctgattttc
    12421 gccatgccgg gttcgaccaa agcgtgccgt accgcatggg aaaatatcat cgcgccgcag
    12481 ctggatgccc gtacgcgtcc gtgtaatttc catccacatt tgaagaaata agtatgtcgc
    12541 aactgaccca tatcaacgcc gctggcgaag cgcacatggt ggatgtctcc gccaaagcgg
    12601 aaaccgtgcg tgaagcgcgg gcggaagcct ttgtcaccat gcgcagcgag acgctggcga
    12661 tgattattga tggtcgccac cacaaaggcg acgtatttgc cactgcgcgt attgtcggta
    12721 ttcaggcggc aaaacgcacc tgggatctga tcccgctgtg tcacccgctg atgctcagca
    12781 aagttgaagt caatttacag gccgagccgg agcacaatcg ggtgcgtata gaaaccttat
    12841 gccgcctgac cgggaaaacc ggtgtcgaaa tggaagcgtt aaccgcggcc tccgtggcgg
    12901 cgctgaccat ttatgacatg tgcaaagcgg tgcaaaaaga tatggtgatt ggtccggtac
    12961 gtttgctggc gaagagcggc ggcaagtcgg gtgactttaa ggtggaagcg gatgattaaa
    13021 gttctttttt tcgcccaggt gcgcgagttg gtgggaacag atgcaaccga agtggctgcg
    13081 gattacccaa ccgttgaagc gttacgccag cacctggctg cgcagagcga tcgctgggcg
    13141 ctggcgctgg aagatggcaa attgctggct gccgtcaacc agacgctggt gagttttgac
    13201 catccgctga ctgacggcga cgaagtagct ttcttcccgc cggtaaccgg aggttaagat
    13261 ggcagaaacc aaaattgttg tcggcccgca gccgttcagc gtaggagaag agtacccgtg
    13321 gctggctgag cgtgacgaag acggtgcagt ggtcacgttt accggtaagg tgcgcaacca
    13381 taacctgggt gacagcgtca aagcattaac cctcgaacac tatccgggga tgactgaaaa
    13441 agcactggca gaaatcgttg atgaagcgcg taaccgctgg ccgctggggc gcgtcacggt
    13501 gattcaccgc atcggggaat tatggccggg cgatgaaatc gtttttgtcg gtgtcaccag
    13561 tgcgcatcgt agcagtgcgt ttgaagccgg gcagtttatt atggattatc tcaaaacccg
    13621 cgcaccgttc tggaagcgcg aagccacgcc ggaaggcgac cgctgggttg aagcccggga
    13681 gagcgatcag caggcggcaa aacgctggta gttttttgtt agccggataa ggcaccgctg
    13741 cgtccggcaa tttcaccgct cagtacatgc gtttacgctg cgtcgatgca cagcctcatc
    13801 attttgcagt atccttaaga tattccttat atcttcagga gatcgtcatg gacagattcc
    13861 cacgttctga ttcaatcgta caaccccggg ctggcttgca aacttatatg gcgcaagtct
    13921 atggctggat gaccgttggc ttgttgctga ccgcatttgt tgcctggtat gcggctaatt
    13981 ccgcggccgt gatggagctg ttgttcacta accgtgtctt tttaatcggt ctgatcatcg
    14041 cgcaattagc attggttatt gtgttatcag cgatgattca aaagctgagc gcaggtgtaa
    14101 cgacgatgct ctttatgctt tattcggcgc tgacgggtct tacgctttcc agtatattca
    14161 ttgtctatac cgctgcttct atcgccagta ctttcgtcgt tactgccggg atgttcggcg
    14221 caatgagcct gtacggttac accacgaagc gcgatttaag tggctttggc aatatgctgt
    14281 ttatggcgtt aatcggcatt gtgctggcat cgctggtcaa cttctggctg aaaagcgaag
    14341 cattgatgtg ggcagttacc tacatcggcg tgattgtctt tgtcggattg acggcgtatg
    14401 acactcagaa attgaaaaat atgggtgagc agattgatac ccgcgacacg tcgaacctgc
    14461 gcaaatattc cattcttggc gcgttaacct tgtatctgga cttcatcaac ctgttcctga
    14521 tgttgttacg gatcttcggc aaccgccgtt aattcttttt caccatcact atcccggcca
    14581 tgtgtcggga tttttttctt actaaaaacg acacttaaca agtaccaggt ataaaatatg
    14641 ccttatgagc gagcggaatc gttttccgta tccataaata caaaagtatt ttgtatgcgt
    14701 ggttttaaat ttattgatgg tgaatattaa tattttttct aaggacaact aaaagaatgg
    14761 aatcatactc gcaaaacagc aataaattag attttcagca cgaggccagg atataaaacg
    14821 gtatatggct cattaccgct ttaggtttgg tggcaaccgc aggactagcc tggggagcta
    14881 agtatctcga aattacggca accaaatatg attcaccacc aatgtatgtc gctatagggt
    14941 tattattact ttgtatgtat ggcttaagta aggatatcaa caagataaat gccgccatcg
    15001 cgggcgtaat atatctgttt ttactctctt tggtggcgat tgtcgttgca agtttagttc
    15061 ctgtatatgc cattatcatc gtgttcagca ctgcgggcgc gatgttttta atcagtatgc
    15121 tggccggttt attatttaat gttgagcctg gttctcaccg ttttatcatt atgatgacgt
    15181 tgacagggtt ggccctggta atcatcgtga atgcggcatt aatgagtgaa cggcccattt
    15241 gggtaataag ttgcttaatg attgtgttat ggccaggcat tatctcgcat ggacgaaata
    15301 agctccttga actggcagga aaatgccata gtgaagagtt gtggagtccg gttcgttgcg
    15361 cttttacagg tgcattaaca ctctattact attttatcgg cttctttggg atacttgccg
    15421 cgatagctat aacgcttgtc tggcaaaggc atacgcgttt ttttcattag ccgtaaatgc
    15481 acatactaaa gaacttaact aaacttcaca tcgccgcttc atttttcgcc cgtagcccag
    15541 tacaaagaaa tagttcacgc tttgaccaag taacagccag ataatcgccc ccattaccat
    15601 ccagttaacg ctggaaatca gcatctgagc gagggcgaat ttccatgaag gcagcaccag
    15661 cttttgccct ttgatggtca tatggcggtg cttcgcgaaa gcgcaaaacc acaaataaac
    15721 cgcgataatc atcagtaagc caatgccgag aatacgcagc gtagtttgat cgacatacca
    15781 gtgatccggc aactccacca cgcctgcggt aaagataatt cctgccagta aaatatagcc
    15841 cagccagttg gtggtaatac tgagcgagaa aatccgcgta atagtgctac cgggcaaccc
    15901 cagacgagag tacaaacgat agcgcatacc aatgccgccg acccaggtac tgagcgtcag
    15961 gttgaaggcg tagcagataa acgacaccag catcacctgg cgcttcgcca gtttgtgacc
    16021 gcagtaaaag cgggcgagca ggtcatagca gccgtaaatc agatagctga cgaccaccag
    16081 cgaccgcact aagcagcgca acgcgattgt agtcgcggat gaccttccag acctcttccc
    16141 agtccacttt tttggcgtag accaccagca acacaatcac cgcgataaaa aacagccagg
    16201 tgaggatctt ctttgctaag cgccagcgcg ggtgtgattt actcatcagg gttttacccc
    16261 cgtgttttca gtttctaccc gatcctgcgt ttccattgtc ggttgtgcgg gcggatcaac
    16321 ctgcgccaga cgtggcgtgt gtgccggaag ccagccaacc agcgccggga agtggcgtaa
    16381 aaagtggaac gccagcacgc ttttggtcag gttccaccag gtgcgtttgg gcagcatggt
    16441 ttcatccacc tgctgacaat ctgcggcgat aatgccgttc agattatcgc gcagcgtctg
    16501 gttaaaatta cgatcgtgga tgatgacatt tgcttcgaga ttcagtgaca aactgagcgg
    16561 atcgagatta ctggacccta ctgtcgccca gtgatcgtcc atcaatgcta ctttgccgtg
    16621 gagcgggcgg cggcggtact caaacacctg aacgccgcct ttaaccagat agttatacag
    16681 caagcgcgcg ccgactctga caatcggcat atccggttcg ccctgaatga tcagtttgat
    16741 ccgcaccccg cggcgtgccg ctttacgcaa ggcgtgtaaa aatcgatagc cggggaagaa
    16801 gtaggcgttg gcgataatga cttcccgccg cgcctgagtg agcattttca gatagtggcg
    16861 ttctatatca tcgcggtgtt cttcgttatc gcgccagacc agcaatacct gcgcgtctcc
    16921 cggctggcgg ttctcttccg ctttgtgatg acgtcgccac cagcgtcgtg ccgcgctctg
    16981 tccaggcagg ttttccagct caaactggag aatatcttcg acaatcggcc cttcaaggcg
    17041 taccgcgtaa tcctgtttag cctctggacc gtagctggac atatgctcgg cggagtaatt
    17101 cagcccgcca ataaaggcga tacgcgcgtc gatcaccaca attttgcgat gcatccggcg
    17161 aaacacattg gtgcgcatac caaaaaggcg agggcgggga tcgtagtagc ggaacactac
    17221 gccagctgcc gtcagttcat tgacaaactc atcgctgaga tccggcgaac cgtagccatc
    17281 cagcaagact tccgctttaa ccccgcgttg cgctgctgcc agtagtgccg catgcagctg
    17341 tttgccgacg ttatcctcaa accagataaa cgtttcaaga atgatgcgtt cttgtgcctc
    17401 gccaatcgcc ttaaacaccg cgggataata ttgctcgccg ttttccagca actggatctt
    17461 attgccttcg cgccagctac atttcataaa tgaatctccg cacttaaagg ggcatgatca
    17521 gaaaggtgtc gccatgtccg cagcggcaac gcggttggcg cgctggcgct ggcatttttg
    17581 acgtagatcc tgtccagtcg tagtagagga aattgcaccg gaaacgtgcg cgccgggcgt
    17641 ccgtgggcgc gggtaaaaat ctcatccagt ccggcctgca cttttaacgg atgattagct
    17701 ttttgccgcc agtcattgaa atcacccgcc accaataccg gttcgccgtc cggtagctca
    17761 ttcacccatt cggcgagcat cgcaagctgc gcctgacggt gcgcctcacg caggcccaga
    17821 tgtacgcaca tcacatgaat cgcttttccg gtcatcggcg gcacaatgcg gcagtagagc
    17881 acgccgcgct tttccgcacc atcgaccgaa acatcgcgat tctcataatg ttcaatggga
    17941 taacgcgaca gtacggcgtt gccgtgatgc ccttccgggt atacggcatt gcgaccgtag
    18001 gcaaaatcgc tccacatagt gtcggcgaga aactcgtagt gcgaggtatc gggccagttt
    18061 tccacatgca gcggatgaac ttcgtgcgcg cccatcactt cctgcaggca aacaatatcg
    18121 gcgctgacgg tacgcacggc atcgcgaagt tccggcaaaa tgaagcgtcg gttaaacgcg
    18181 gtaaagcctt tgtgaatgtt gatggtgagc accttgaacg aaaattgttg tgtttgatcg
    18241 ggcataattt tcctgtcttt gcctcttatc tcattgaaat agtgtagtcg gcgtcacaaa
    18301 aaggtgcggt cttacggaat tttccgtaaa gttcggtact ctgagtaagt agagataaat
    18361 tcttcaggag agaagccatg aagtggcaac aacgtgttcg tgtcgcaacg ggtctaagtt
    18421 gctggcagat tatgttgcat ttactggtag tggcgctgct ggtggtgggc tggatgagta
    18481 agactctggt tcacgtcggc gtgggattat gcgcactgta ttgtgtcacg gtagtgatga
    18541 tgctggtgtt tcagcgccac cccgagcaac gctggcgtga ggtggcagac gtgctggaag
    18601 agctgaccac gacctggtat tttggcgcag cgctgattgt gctgtggctg ttgtcccgcg
    18661 tactggaaaa caactttttg ctggcaattg cagggctggc gatccttgct ggcccggcgg
    18721 tagtgtcttt gctggcgaaa gataagaagt tacatcacct tacgtctaaa catcgcgtac
    18781 gccgctgacc ctgtcgtggc cgttatcacc agtagcggcc acaaactatt ccacacaata
    18841 tccagactcg catccttcaa ataaatctgc ttggtaatgt ccgtaaagtg gcgaataggg
    18901 ttaatccacg tcaggttttg cagccatact ggcatgtttt ccaccggaga aacgtaaccg
    18961 gaaaggagaa tggcgggcat cataaaaaca aacacgccga taaacgcctg ctgttgtgtc
    19021 gagcagagtg atgaaatcaa cagaccgaat cccaccagcg ataaaccgta aatcaccatc
    19081 gtaaagtaga acagcgccag cgatccggcg aaggggattt gatacgccca gataccaatc
    19141 gccagcacaa tggtggcctg gaaggtggcg acaattaacg ccggtacggc tttgccgatg
    19201 aagatctgcc aggtggtgag cggcgaaacc agtagctgat cgagcgtacc ttgttcacgt
    19261 tcgcgggcga cggaaagtga agtgacgatc attacgccga tagtggtgat catggcgatc
    19321 agcgacggca ccacaaacca tttgtagtca agattcgggt tataccagtt gcgtaccacc
    19381 agctcgctgt tgttaggttt cggttttcct tccagcagct cctgctgata atttttgacg
    19441 atctgttgca ggtagttggc ggcaatttgc gcactgttgg agttacgccc gtcgaggatc
    19501 aactgcaacg gcgcggtctg gaaggtatcc agtttgcgcg agaagtcagc cgggaaacgc
    19561 accagtagta acgccttttg tgtgtcgatg gttgggcgga tctcctgtgg gcttttcagc
    19621 agcagcacat gggtaaaggc gctggcgcgg gcaaaacgtt gggtcagctc caccgaatgc
    19681 tcgccgttat cttcatcgta gatggcgatg gtggcgttag tcacttccag cgtggcggcg
    19741 aacgggaaca ggatcacctg aataagcaca ggtaaaatca gaatcgcgcg ggtttgtggt
    19801 tcgcgcagca acgactgcaa ctctttgcgg attaacgtcc ataagcgatg aaacatgctc
    19861 ttctccctaa tccagccgac gtttggtttt cagccacgtc aggccgataa acatcaccgc
    19921 cgaagcgatc aaaaacagca cgtttaccac cagcaccact ggaatattcc cggcgaggaa
    19981 caggctttgc agggtgctga cgaaataacg agcgggaata atgtacgtca ccgcgcggat
    20041 caccgcgggc atactgtcga tctgaaaaat aaagccggaa agcataatcg acggcaggaa
    20101 cgcggcgttc agggcgacct gagcggcatt gaactggttg cgggtaatcg tggaaatcag
    20161 cagccccatc cccagggtac tgagtaaaaa caggctggag ataaaaaaca gaataagcag
    20221 cgaaccgcga tacggcacgc cgagaataaa cactgacacc agcatacaca gcagcatcgc
    20281 cagcatcccg agaaagtaat aagggatcag cttacacagc agcagttccg tgcgggtaat
    20341 ctccgtagag agcagagcct ccatggtgcc gcgttcccat tctcgcgcca ccaccagcga
    20401 ggtgagaatc gcgccgatga ccgtcatgat gatggtcact gcaccgggga taatgaagtg
    20461 ctggctaatc gccgccgggt taaaccagta gcgggtttgt acatcaataa gcggttcaaa
    20521 agtctgcccg ttgtcctccg ctcgctgcat ttgccagatc tgccagatcc cttcgacata
    20581 cccctgtaca aagttagcgg tattcggctc actgccgtcg gtgatcacct gaatcggtgc
    20641 ggtggcgttg gcgcgctcca tctgttccgc aaaatccacc ggaataacca ccagaccgcg
    20701 aattttcccc gcctgcattt tggcgatcag ttcctgacgg ttatcgctga tggtggcgtc
    20761 gatgtagggc gaaccggtca tggtgtgggt gaaatccagc gccgcttcgc tacgctgttc
    20821 cagcaagatc ccgacccgca gcttgctgga gtcgaggtta atgccataac caaaaataaa
    20881 cagcagtagc agtgggatca ctaccgcaat cagccagcta ctcggatcgc gaacgatctg
    20941 ccgcgtctct ttgacgcaca gcgcccgcac gcgacgccag gaaaggatcg ggttactcat
    21001 tgctatgctc cttatcccag tcgtggatca actgaataaa ggcttgctcc atggtgggat
    21061 cgggttgctc atcgttagcc gactgtgctt tcaaatcgtc cggcgtaccg ctggcgatta
    21121 atttcccacg gtacaccagg ccgatgcggt cgcaatattc cgcttcatcc ataaagtggg
    21181 tggtgaccat caccgtgacg cctttctcta ccatgctgtt gatatgtagc caaaattcac
    21241 ggcgggtgag gggatcaacg ccggacgtcg gttcgtcgag aaacagaatg tccggttcat
    21301 gcatcagcga acaggccagc gccagccgct gtttaaaacc taatggcagt tcatcggtgg
    21361 cgtgggaggc gatacttttc aggccgaacg cctcgctcat gcgggagatt ttttcgttct
    21421 gcgcccgacc gcgtaagcca tacacaccag agaaaaagcg taaattctgt tcgaccgtca
    21481 ggttaccgta gagcgaaaac ttttgcgcca tatagccgag atgctggcgc gctttaccag
    21541 aactctcttt cagatccatc cccagtacca gcgcctgacc agaggtcggc accagcaaac
    21601 cgcacatcat cttaaaggtg gtcgatttgc ccgcgccgtt tggccccagc aaaccaaaaa
    21661 tctccccgcg tttaacggca aagttgacgt gatcggtggc ggcaaaatca ccaaatttct
    21721 tggtcagttc tttcgcttcg atcaccgtct cgccgggtgt gccttctacc gtatgtaata
    21781 ttgcgcccag cggcgattcc gaggttccgg caccgcccag caaatcaata aacgcatctt
    21841 caaaacgcgg cgtagtttcg ttgatgttga tttccggcat cccgtcggca tggcgaatat
    21901 cgtctggtgt ggtctctttt ttgaggatca gacgtaccga tttcccctga atcatgccgt
    21961 cgctgacctg cggcagtttc aatgcccgtt gcaacagttt gcggttgccc tcgtgtggac
    22021 ttgtcatcag aaagctgcgt ccggccatgg tttgtgtcag ggcttttggt tccccctgat
    22081 acagcaactc gccttcattc atcagtaaca cgtcacggca ctgctcggct tcgtcgagat
    22141 acgaggtact ccagaggatt aacatccctt cgcctgccag ctcatgcacc atctgccaca
    22201 gttcgcgccg tgagataggg tcaacgccga cgccgggttc atcgagcagc aacactttcg
    22261 gttcgcccac cagggtacag gccaggccga gtttttgttt catcccaccg gagagcttgc
    22321 ccgccaggcg tccggtaaac ggcccaagag acgtaaactc cagcaggcga gcaaaagttt
    22381 gcttacgtgc ctcgccggtg acgctgcgca aatccgcgta cagattgagg ttctccatca
    22441 ccgtgagatc ttcatacaga ccaaatttct gcggcatata gccgagcacg gcgtgcagcg
    22501 cgccgtcgtt tttgattgga tcaaagccaa tcaccgtggc attgccgctg tcgggtttca
    22561 gtaatcccgc cagcatccgc attagcgtgg ttttacctgc accatccggc cccaccagcc
    22621 ccgtcacata accggcgtga atggtacaat cgagcggcgc gacggcgggc ttgtccatgc
    22681 ccggaaagcg tttttccagg ccgttcagcg tgataacggc atcattcatg tcctgcctcg
    22741 ttaccgaatt gcaccgtcac cggcattccc tggcgtaacg catcatcggc gtcggtcacc
    22801 acaatacgca ggcgatagac gaggtcggta cgcagatccg gtgtttcgac ggttttcggg
    22861 gtaaattcag cagtcggcga aacgaaacca atctgcccgt gatacggctt gtccgggcga
    22921 ccatcggtat aaagcagcac tttgcgcccc ggctgggcct ggtcaagata ccgttcatca
    22981 acataagcgc gcacccacac cggacgcgtt agtgaaacgg taaacaccgt gccaccttca
    23041 ttgaggaccg tgcctggctc caccgcgcgc gttaacagcg tgccatcaga cggggctatc
    23101 aacgttgagt cctgtaaatt caactccgcc tgcgccagtt gcgcctgcgc ctgttcgagg
    23161 ctggctttcg cctgggcgat gtcctgttca cggttaccgg aacggtactg acgcaattta
    23221 tcctgtgctg atttcagcgt tgcctgcgcc tggtcgcgcg aggagcgggc attttccagg
    23281 tcatttgccg aaatagtgcg gcttttccac agtccttgct ggcggttata gaagttctgc
    23341 gcatagtcat aggcggcttg cgcctgtttc accgctgcgg cggcctgagc gatttcttca
    23401 tcgcgatacc cggcaagcat cagatcatac tgcgcctgtg ccaccgagac gcccgctttc
    23461 gcctgcatca gggcaatctc atacggcttg tgatccagtt cgcccagcac ctggcccgct
    23521 ttgatagcat caccttcgtc caccgccagc gattcaacgc gccccccaac acggaaacta
    23581 agattcaccg tacgaatatc cacgttgcca tacagcgtca ggccgttatc ctggcggctt
    23641 tgataccacc agtagcctcc ggcaaccacg gcggcaagta ccactaccgc cagtccgatc
    23701 acgacaggtt ttttcatcac tacaaactcc tttgcgataa tccttgcaaa atcaggtcga
    23761 tatgacaggt caccgtctgg ttgatcagtt cggtcttttc ttcatcgaac gcggtccagc
    23821 cggtacgtaa cagaattgtt tctttgccaa gacggaacgc cagaatctca ccaatcaacg
    23881 catgggtatg aaggatcatg cgggtgtcat tggcgtcgct gccggtccag gcggcaatca
    23941 ggcgtgtcag gtggctgtgt agcggactaa tcacctgttc gtgcaccagg tggtaggctg
    24001 ccgtgggaga gagctgctca cgggagataa acttgctgag gttgacggtg tcatcctggg
    24061 tgagcagctt aatcatgttc ctgcaggcgc gaaggatcag ttcacggatg gcagcccgat
    24121 caggctgtgg ttgggcgaac aagcgttcgg cttcctcggc atgcggacgg aactgctcgc
    24181 caataaaatc ggcaatccac tgggcgcagg cgaggtacaa atcttctttc gaaccgaagt
    24241 agtaggtgat ggcagcgata ttctgcccgg cctgggcggc tatctcgcgg gtggtggcgt
    24301 tcattccata ttcaccaaac tgcgccagtg cggcagcaat cagctgtttt ttcgcctgtt
    24361 cacccttgat tgtcatggca ggattattca tcgcacagtc cattcttaat caaatgattg
    24421 attaagatta tgactccata tgggagttgt ccagtatggc taagaatttt agctacgcca
    24481 gtcacaggga taatttatgc gctgcgtcac aaaaactgct acactccgct cgctcatgac
    24541 attgtggttt ttgtcatttt ccttttcagt atctccctga aaactacacc ggtaacggtc
    24601 ggggcggttc ggagtagtca tgtctttcga ttctttgggt ttaagccctg atatcctgcg
    24661 cgccgttgcc gagcagggtt accgtgaacc cacccctatt cagcagcagg cgatccctgc
    24721 ggtgctggaa ggccgcgacc tgatggctag cgcccagacc ggcaccggca aaacagcggg
    24781 ctttacgctg ccgctgttgc aacacctgat cactcgccag ccgcacgcca aagggcgtcg
    24841 tccggtacgt gcgctcattc ttaccccgac ccgtgaactg gcggcgcaga ttggcgaaaa
    24901 cgtccgtgat tacagcaaat acctgaacat tcgttcgctg gtggtgtttg gtggtgtgag
    24961 tattaacccg cagatgatga aactgcgtgg cggcgttgat gtgctggtgg caacgccggg
    25021 acgtttgctg gacctggaac atcagaatgc agtgaagctg gatcaggttg aaatcctcgt
    25081 cctcgatgaa gctgaccgca tgctcgacat gggctttatc cacgatatcc gtcgcgtgtt
    25141 aacaaaacta cctgcgaagc gccagaacct gttattctcc gcgaccttct ctgacgatat
    25201 taaagccctg gcggaaaaac tgttgcacaa cccgctggaa atcgaagtgg cacgccgcaa
    25261 taccgcgtct gatcaggtga cccagcacgt tcactttgtc gataagaaac gcaaacgcga
    25321 attgctgtcg cacatgattg ggaaagggaa ctggcagcag gtgctggtgt ttacccgtac
    25381 caaacacggc gctaaccatc tggctgaaca gctcaataaa gatggcatcc gtagtgcggc
    25441 gatccacggc aataaatcgc aaggtgcgcg tactcgtgcg ctggctgatt ttaaatcggg
    25501 cgatattcgt gtactggtgg caactgacat cgctgcgcgc ggcctggata ttgaagagct
    25561 gccgcacgtc gtcaactatg aactgccaaa cgtaccggaa gattatgtcc atcgtatcgg
    25621 gcgtaccggt cgtgcggctg ctaccggtga agcgttgtcg ctggtgtgtg ttgatgaaca
    25681 caaactgctg cgtgatatcg aaaaactgct gaaaaaagag atcccgcgca ttgcgattcc
    25741 gggctatgag ccggacccgt caatcaaagc cgaaccgatc cagaacggtc gccagcaacg
    25801 tggcggcggc ggtcgtgggc aaggtggtgg tcgcggtcaa cagcaaccac gccgtgggga
    25861 aggtggcgca aaatctgcaa gcgcgaaacc tgcagaaaaa ccgtctcgcc gcctcggcga
    25921 tgccaaaccg gcaggcgaac aacaacgtcg ccgccgtccg cgtaaacctg ccgctgcgca
    25981 gtaatctttt atgccgggct atgcccggca tcaggctgat gaacaaacgc aaaactgcct
    26041 gatgcgctac gcttatcagg cctacgtgaa ctctgcaata tattgaattt gcatgctttt
    26101 gtaggccgga taaggcgttt acgccgcatc cggcatttca caacaagcac ttgtcagcaa
    26161 tttgagaaca cgggaaaaaa tttatctgtt ttaccgccca taagccaccg agacgttact
    26221 tctctatagc caattgttct cgcaactcca ttaaaaggta gcccagtcga ttcttgccct
    26281 taccattacc accgtctccc cagtaagcat cgttttccgt atgctcaacc agttttgcgg
    26341 gcgcggttgc cagcaagagc acacgcagtt ctgcatgctg ttcgaattta gcacgaagtg
    26401 ctttacgcat cacctgttct ttaaccgact cccagttttt acgaagtggc ttggaacgat
    26461 tacgccccat gcgagcggcg accatggggg aagaaacccg acggatctct tcgcggtatt
    26521 tttcatcaag gaatttttgt gcctgaaaat agtgttcgga ggtgggccag gttttcccgt
    26581 caaccttgat gggccaggcg gcaaagttag aaaaatcacc gtagtcatcg ctggtgctgt
    26641 agaaatttat gatggtgtct tgcatgacgt gttggatcct ttgtgctcga acgggcatta
    26701 aaccgcatta tgttggtggt tattgcgagc cgctttccag aaacagaaaa accattaccc
    26761 ctgaaaaccg aaaaatgcca caatattggc tgtttataca gtatttcagg ttttctcatg
    26821 gcattaaccg ccgcgcttaa agcgcaaatt gccgcctggt ataaggcgct tcaggaacag
    26881 atccccgact ttattccccg tgtgccgcag cggcagatga ttgcggacgt cgccaaaacg
    26941 ctggccggag aagaagggcg gcatctggcg attgaagccc ccaccggcgt tgggaaaacg
    27001 ctctcctatt tgatccccgg catcgccatt gcccgcgaag agcaaaaaac gctggtggtg
    27061 agtaccgcca acgtggcgtt gcaggatcag atttacagca aagatttacc gctgctgaaa
    27121 aagatcattc ccgatcttaa attcactgcc gctttcgggc gtgggcgcta cgtttgtccg
    27181 cgtaatctga cggcgctcgc cagcactgaa cccacgcaac aggatctgct ggcgtttctt
    27241 gacgacgaac tgacggcaaa taatcaggaa gagcaaaaac gttgtgcgaa gctgaagggc
    27301 gatctcgaca cttatagatg ggatggtctg cgcgatcata cggatatcgc tatagatgat
    27361 gatctctggc gtcgtttgag taccgacaaa gccagctgcc tcaaccgtaa ctgttactac
    27421 tatcgtgaat gcccgttttt tgttgctcgt cgggagattc aggaagcgga agtggtggtg
    27481 gcaaaccatg cgctggtgat ggcggcgatg gaaagcgaag ccgtactgcc tgacccgaaa
    27541 aatctgctgc tggtgctgga cgaaggtcat cacctgccag atgtggcccg ggatgcgctg
    27601 gagatgagcg ctgaaatcac cgcgccctgg tatcgactac agctggactt gttcacgaaa
    27661 ctggtcgcta cctgcatgga gcagtttcgc ccgaagacca tcccaccgct ggcgatccct
    27721 gaacgtttga atgcgcattg tgaagagttg tatgagctta tcgcctcgtt aaacaacatt
    27781 ctcaatctct acatgcctgc cgggcaggag gcagagcacc gttttgcgat gggcgaactg
    27841 cctgatgaag tgctggagat ctgccagcgg ctggcaaaac tcaccgagat gctgcgtggt
    27901 ctggcggagt tatttcttaa cgatttaagt gagaaaaccg gcagccatga cattgtacgc
    27961 ctgcatcggt tgattttgca gatgaaccgc gcgttgggga tgttcgaggc gcaaagcaaa
    28021 ctctggcggc tggcgtctct ggcgcaatct tccggtgcgc cggtgaccaa atgggcgacg
    28081 cgggaagagc gcgaagggca actgcatctc tggtttcact gcgtggggat acgtgtcagc
    28141 gatcagctgg aaaggctgct gtggcgcagt attccgcaca ttattgtcac ctccgcaacc
    28201 ttgcgttcgc tgaatagttt ttcgcgtttg caggagatga gtggtctgaa agagaaagcg
    28261 ggcgaccgtt ttgtggcgct ggattccccc tttaaccact gcgaacaggg caaaattgtt
    28321 attccccgga tgcgcgttga gccttccatc gacaacgaag agcagcatat tgccgaaatg
    28381 gcggcctttt tccgtaagca ggtggagagc aaaaaacatc tcggtatgtt ggtactgttt
    28441 gccagcggac gggcgatgca gcgctttctc gactatgtga cggatttacg tctgatgttg
    28501 ctggttcagg gcgatcagcc gcgttaccgt ttagttgaac tgcaccgcaa acgcgtcgcc
    28561 aacggtgagc gcagcgtgct ggtgggctta cagtcatttg ccgaagggct tgatttgaaa
    28621 ggtgatctgc tcagccaggt gcatatccac aaaatcgctt ttccgcccat cgacagcccc
    28681 gtggtgatca ccgaagggga atggctgaaa agcctcaacc gctatccgtt tgaggtgcaa
    28741 agcctgccga gcgcctcgtt taacctgatt cagcaggttg ggcgactgat tcgaagccac
    28801 ggttgctggg gcgaagtggt tatctacgac aaacgcttgc tgaccaaaaa ttatggcaag
    28861 cgactactgg atgcattacc ggtatttccg atagagcaac cggaagtccc tgaaggtata
    28921 gttaaaaaga aagaaaaaac gaaatcccca cgccgtcggc ggcgttaatg atgtgagtca
    28981 ggtaaggagt cgtaaatgga ctatcgcaaa atcattaaag agatcgggcg cgggaaaaac
    29041 cacgcgcgcg atttagaccg ggatactgcg cgcggtctgt atgctcatat gctcaatggt
    29101 gaagtccctg accttgagtt gggcggcgta ttgattgcgc tgcgtatcaa aggagaaggg
    29161 gaagcagaga tgctcggctt ttacgaagcc atgcaaaatc acaccatcaa gctgacgccg
    29221 ccagcaggca agccgatgcc gattgtcatc cccagttaca acggcgcgcg taaacaggcc
    29281 aacctgacgc cgttgttggc gattctcctg cacaaactcg gttttcctgt ggtggttcac
    29341 ggggttagcg aagatccaac ccgcgtgctg actgaaacca tttttgaatt gatgggtatt
    29401 acgccaacgt tgcacggcgg gcaggcgcag gcgaagctcg acgaacatca accggtgttt
    29461 atgccagtcg gcgcgttttg cccgccgctg gaaaaacaac tggcgatgcg ctggcggatg
    29521 ggcgtgcgta acagtgcaca taccctggcg aaactggcga cgccatttgc cgaaggtgcg
    29581 gcgctgcgtc tttccagcgt ttcgcatccg gaatatattg gacgcgtcgc gaagttcttt
    29641 agcgatatcg gcgggcgggc gctactaatg catggtactg aaggtgaagt gtatgctaat
    29701 ccgcagcgct gcccgcagat caatctcatt gaccgtgaag ggatgcgggt gctgtatgaa
    29761 aaacaggaca ctgctggtag cgagttactg ccacaagcaa aagatccgga aaccacggcg
    29821 cagtggattg agcgttgcct tgctggcagc gaaccgattc ccgaatcgct gaaaatccag
    29881 atggtttgct gcctggtggc tacgggtgaa gcggcaacta tcagcgacgg cctggcgcgc
    29941 gttaatcagg cattttaatt cttttctcag ccggatgagc catgtcatcc ggcttttacc
    30001 ccaccgcaat atgaaattcc tgcatcttta ttgaccttcc cacgcccggc gtgcagcata
    30061 aaaatacaac aaacacataa cataaacagg agttaaccat ggaaagtggt catcgctttg
    30121 atgctcagac gctgcacagt tttattcagg ctgtatttcg tcagatgggt agcgaggaac
    30181 aagaagcgaa attagttgcc gatcatttaa tcgcggcaaa cctggcaggg catgattcac
    30241 atggtattgg catgatccca agctatgtac gctcctggag tcaggggcac ctgcaaatta
    30301 accatcatgc caaaaccgtt aaagaggcgg gggcggcggt cacgctcgat ggcgatcgcg
    30361 catttggtca ggtcgcggca catgaagcga tggcgctggg gattgagaaa gcgcatcagc
    30421 acggtattgc cgccgtggcg ctacataact cgcatcatat cggccgtatc ggttactggg
    30481 cggagcagtg tgcagcggcg gggtttgtct ctatccactt tgttagcgtg gtcggtattc
    30541 caatggtcgc gccgttccac ggtcgcgaca gccgctttgg caccaatccg ttctgtgtgg
    30601 ttttccctcg taaagataat ttcccgctgt tgcttgatta cgccaccagc gccattgcat
    30661 ttggcaaaac ccgcgtcgcc tggcataaag gcgtccccgt gccgccaggt tgcctgattg
    30721 acgttaacgg cgtgccgacg accaatccgg cggtaatgca ggagtcgccg ttgggttcgc
    30781 tgttgacctt tgccgaacat aaaggctacg cccttgcagc gatgtgtgaa attcttggcg
    30841 gggcgctttc cggcggtaaa acgacgcatc aggaaacgtt acaaaccagt cccgatgcca
    30901 ttcttaactg catgaccact atcatcatca acccggaact cttcggcgcg ccggattgta
    30961 acgcgcagac cgaagccttt gccgagtggg tgaaagcctc gccgcatgat gatgataagc
    31021 cgattttgct accgggcgag tgggaagtga acacgcgtcg cgaacggcag aagcagggga
    31081 ttccactgga tgcgggaagc tggcaggcca tttgtgatgc agcgcggcag attggtatgc
    31141 cggaagagac gttgcaggct ttctgtcagc agttagccag ctaaaaaaaa gcccgtccag
    31201 tggcggacgg gcaaacaagg gtaacatagg atcaatgagg gttagagcat atgcgtctgt
    31261 cggcaaacag acagggaaat acttgtgctg gacgtagcgt aaacgcctga tccgtccaac
    31321 ggttcggatt ttgtaggcct gataagacgc ggtagcgtcg catcaggcat ctgttgccgg
    31381 atgcggcgta aacgccttat ccgtcctacg aattacttat aaattaccgc agtaccgctt
    31441 aatttattgt tgttggtggc ggaagtgatg ctgtatccac tggcaccggc tgccgcggct
    31501 ttctcagcca gtttcgcttc cagggcatcg agggtggatg cgccatcggc agaaaccacg
    31561 ccgattttat tcatgttctg tgcctgggat gccgttatcg gttccgccgc gaacacgcca
    31621 aatgacagag ttgaaagagc catagcagca acaacagtat tgatagtttt cataattaat
    31681 ctctcgcagg tgatttttgt ataagtgacg ttgtttcgtc gatgtgatga gtatcacgtt
    31741 tttttacgag agataaaatc gaagagaatt gaccgccttg ttcaaataaa ttgattgata
    31801 tctaacatac tgataaacca tttatttgtt gtacttatga acttgtttta taccgcgtgg
    31861 cagtcacagc agcgttaaag gcacggaatg ccacgcaaaa taaaaattat actcgctatg
    31921 catctcgcca gttgtagtta acgtaactgg ctgtctttcg aacctctgcg attatatcct
    31981 gtataagctg gtttttgtaa atctttctcc tgctgacaat gaatacataa gcgcacgcca
    32041 ggaatggctt cccgacgggc ctgcgggatg ggggcaccgc actcttcaca ttcatccagg
    32101 ctttcgccgc gcggaatttc accccgagcg cgggcaatcg catcttcaat tgtactgttg
    32161 atctgttcgt tgacggcgtc atcgttagcc caaccggatg ccatatcgac ctccccatat
    32221 caatacttgt acagttaagt gtagctaatc cagggacgaa ctcgggcagt tcaagcatca
    32281 gatctccgac cattcccgca gcagattatg ataaagatta agcagcgaca ggatctcttc
    32341 actttcgccg tagcggcttt tcagcgactg aatattgttg tccagttcaa acagcatggc
    32401 gcgcttttta tcatcgcgga tcatcgactg gatccacata aatgatgcca ctcgtacgcc
    32461 gcgggttacg ggtggcacgc aatgctggct gctggaggga tacaacacga gatcgcctgc
    32521 cgggagtttt acccgatgtt gtccgaaggt gtcattaacg accagttcgc cgccgtcgta
    32581 gctttgtgga tcgcttaaaa acagcgtggc agaaaggtca gtacgcatcc agccgttttg
    32641 tggatggctg cgtactgcgc catccacatg gaaaccatag gtttcattgt tctgatagcg
    32701 attaaacagc ggcgtggaaa gggtacgcgg caaggccgcg gcaaagaaca aagcatgttg
    32761 gttaaccgcg ttcagcacct cattttgcag ggcggcgtat aacgtgctgc gggtgtcgac
    32821 ctgttgattg ttcttaactt gcgcaccttg tgcgccggtg gtgacgcgtc catccaccca
    32881 ttcggcttgt tccagttgtt cgcgaaaccg ggcgacgtcc tgtggcgata acacgccggg
    32941 aatgtggtac atcatacaag ttctccaaaa agtggggcct gcgccccaca tctgaatcag
    33001 aaatgcatat tggctgtgag caagaaggtt cttggctcgc ccgggtgata acggtagccg
    33061 ctcttgttga ttgaggcgac gtaatcagta tcaaacaggt tgtagacgtt tagctggaag
    33121 tcgagattgc gattaacccg ataccccagt ttggcatcgg cgacccagta accttcggta
    33181 aatgctggcg ttcccaccgc gccgtctgaa cctttatgca tactgccgat atagcgtgcg
    33241 cccgcgccaa cagagatatc gtcggttgcc tgatattggc tccataaggt gaaggcgtgc
    33301 tccggggtat acggcagcga tgaggaacca tcctgggcaa catctttgcc gtttttgatg
    33361 gttgcttttt gctgggtata gccgccaatc acctgccacg cgggagtgat attcccggcc
    33421 acggatatct catagccttc gacgcgtttc ttaccgtatt gcgagtaagt tccgtcatca
    33481 ttttgctcaa cttcattttc gatatcagta cggaacagcg cggcggtgag caacagacgt
    33541 ttatccagaa cctgccattt ggtgccaatc tcgctggtgt tggctttttg cggtttaaaa
    33601 tcggtgcggt tggcactgtt accgctgcca gactgcgcaa gggcgaagtt gttgccgccc
    33661 ggaggctgct gggaaacggc atagttaata tagacattgc cgttttccgt caggtgatac
    33721 agcgccccgg ctttccagtt catcagattg cccgacttgg cggtgtcgac ggtggtgacc
    33781 ggagaacctt ttgccacacc agttgggcag gtgatggcac cgcgtccgct gccgccgcag
    33841 gcggtggcac tgtcatattc agtatgataa ttatccagac ggatcccgcc gttcagctca
    33901 aaatcacggg tgatttgcag cgtatcaaag gcgtaaattg cgaaggtatc cgtctgacca
    33961 ttggcgtttg cgccgttgcg cgtcaggccg ccgggatgaa tgctgctgtc aggatgataa
    34021 atatttaccg cgggtaacgt caccggatta acgccgtagt tagtctgcgt ttcacgggta
    34081 aattccacgc cggtactgac atcatgaccg atagaaccgg tatagaacgt cgaggtcagg
    34141 ttggtctggt tggtgagaat tttattactc acatctttgg tattcgccgt gcgtgaccag
    34201 gttcagctat tcacatcgct ggtgggctga gtaatattcg acgccccgcc cataatcgcc
    34261 gtcatcaggt aatcctgctt tacgcgcgac caacgggtag tattgcgaat ggtggtgtta
    34321 tcgttgatgt cgtgctcaaa acgcatggtg gcggtgtcgg tggtcgaatc gtcgtaatcg
    34381 gaatccgtgc cgtaaaagtt atgagtatca acttttccgg aatgattcag ggccgccgtt
    34441 cccgcagatg gggcagaata gcccggcaaa ccgatggtcg gaatgccgcc gtctggcgtg
    34501 ttgtgctggg tgacatgcag ataattaaga tacaaacgat tcgctgtacc aaggccaaaa
    34561 gcgacagaag gggcgacgcc gtaacgctca tttttgactt tgtcgcgtcc ggcatcgtgc
    34621 gttttttcgc ccattacatt caggcgcacc gcggtggtat caccaatgac ctgattgacg
    34681 tccagcgtgc cgcggcggaa ccaggcgctg ccaatactgg cggaggcgtc aatgccggaa
    34741 tcattgcgcg gctgtttgct gatcatattg atcgagcccg tcggtgcgct gcgcccgtag
    34801 tcggtgccgg acggcccttt aatcacttcg acctgctcgg tattgaaggt gtcgcgcgag
    34861 acgctgccga tatcgcgaat gccatcaata taaatactgt tagaggtatc ggcaccacgc
    34921 atataaatgg cgtcgccagt ggtggagtta ccgttctcac ccgcaaaaaa cgcacccacg
    34981 c
//
LOCUS       DAKISA010000039        34943 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.039, whole genome shotgun sequence.
ACCESSION   DAKISA010000039 DAKISA010000000
VERSION     DAKISA010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34943
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.039"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..467
                     /locus_tag="OVW45_001940"
     CDS             <1..467
                     /locus_tag="OVW45_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCV5526397.1"
                     /translation="GTSVTGSTVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNIETK
                     LVSTKVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKDYDP
                     RGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            580..1374
                     /gene="panB"
                     /locus_tag="OVW45_001941"
     CDS             580..1374
                     /gene="panB"
                     /locus_tag="OVW45_001941"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCV5526398.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPIELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1386..2237
                     /gene="panC"
                     /locus_tag="OVW45_001942"
     CDS             1386..2237
                     /gene="panC"
                     /locus_tag="OVW45_001942"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCV5526399.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(2529..3425)
                     /gene="rpnC"
                     /locus_tag="OVW45_001943"
     CDS             complement(2529..3425)
                     /gene="rpnC"
                     /locus_tag="OVW45_001943"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCV5526400.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMVFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            3699..4079
                     /gene="panD"
                     /locus_tag="OVW45_001944"
     CDS             3699..4079
                     /gene="panD"
                     /locus_tag="OVW45_001944"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCV5526401.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(4083..5312)
                     /locus_tag="OVW45_001945"
     CDS             complement(4083..5312)
                     /locus_tag="OVW45_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCV5526402.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQVRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(5376..5816)
                     /locus_tag="OVW45_001946"
     CDS             complement(5376..5816)
                     /locus_tag="OVW45_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCV5526403.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5921..6691)
                     /locus_tag="OVW45_001947"
     CDS             complement(5921..6691)
                     /locus_tag="OVW45_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCV5526404.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6688..7614)
                     /locus_tag="OVW45_001948"
     CDS             complement(6688..7614)
                     /locus_tag="OVW45_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCV5526405.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7723..8385
                     /gene="can"
                     /locus_tag="OVW45_001949"
     CDS             7723..8385
                     /gene="can"
                     /locus_tag="OVW45_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCV5526406.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8426..8962)
                     /gene="hpt"
                     /locus_tag="OVW45_001950"
     CDS             complement(8426..8962)
                     /gene="hpt"
                     /locus_tag="OVW45_001950"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCV5526407.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILRDE"
     gene            9168..11558
                     /gene="gcd"
                     /locus_tag="OVW45_001951"
     CDS             9168..11558
                     /gene="gcd"
                     /locus_tag="OVW45_001951"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCV5526408.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWCSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKINEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11605..13155)
                     /gene="cueO"
                     /locus_tag="OVW45_001952"
     CDS             complement(11605..13155)
                     /gene="cueO"
                     /locus_tag="OVW45_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCV5526409.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRTVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGM
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            13321..13668
                     /locus_tag="OVW45_001953"
     CDS             13321..13668
                     /locus_tag="OVW45_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCV5526410.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13774..14640
                     /gene="speE"
                     /locus_tag="OVW45_001954"
     CDS             13774..14640
                     /gene="speE"
                     /locus_tag="OVW45_001954"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCV5526411.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGIVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSYRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14656..15450
                     /gene="speD"
                     /locus_tag="OVW45_001955"
     CDS             14656..15450
                     /gene="speD"
                     /locus_tag="OVW45_001955"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCV5526412.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15488..15850)
                     /locus_tag="OVW45_001956"
     CDS             complement(15488..15850)
                     /locus_tag="OVW45_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCV5526413.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(16025..18622)
                     /gene="acnB"
                     /locus_tag="OVW45_001957"
     CDS             complement(16025..18622)
                     /gene="acnB"
                     /locus_tag="OVW45_001957"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCV5526414.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18977..20734
                     /locus_tag="OVW45_001958"
     CDS             18977..20734
                     /locus_tag="OVW45_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HCV5526415.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVETQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(20805..22229)
                     /gene="lpdA"
                     /locus_tag="OVW45_001959"
     CDS             complement(20805..22229)
                     /gene="lpdA"
                     /locus_tag="OVW45_001959"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCV5526416.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22554..24446)
                     /gene="aceF"
                     /locus_tag="OVW45_001960"
     CDS             complement(22554..24446)
                     /gene="aceF"
                     /locus_tag="OVW45_001960"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCV5526417.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPASAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKLTPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24461..27124)
                     /gene="aceE"
                     /locus_tag="OVW45_001961"
     CDS             complement(24461..27124)
                     /gene="aceE"
                     /locus_tag="OVW45_001961"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCV5526418.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYIPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27285..28049)
                     /gene="pdhR"
                     /locus_tag="OVW45_001962"
     CDS             complement(27285..28049)
                     /gene="pdhR"
                     /locus_tag="OVW45_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCV5526419.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28590..29963
                     /gene="aroP"
                     /locus_tag="OVW45_001963"
     CDS             28590..29963
                     /gene="aroP"
                     /locus_tag="OVW45_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCV5526420.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(30006..30860)
                     /gene="ampE"
                     /locus_tag="OVW45_001964"
     CDS             complement(30006..30860)
                     /gene="ampE"
                     /locus_tag="OVW45_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCV5526421.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30857..31408)
                     /gene="ampD"
                     /locus_tag="OVW45_001965"
     CDS             complement(30857..31408)
                     /gene="ampD"
                     /locus_tag="OVW45_001965"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCV5526422.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31496..32389
                     /gene="nadC"
                     /locus_tag="OVW45_001966"
     CDS             31496..32389
                     /gene="nadC"
                     /locus_tag="OVW45_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCV5526423.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVSTRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32592..33032
                     /gene="ppdD"
                     /locus_tag="OVW45_001967"
     CDS             32592..33032
                     /gene="ppdD"
                     /locus_tag="OVW45_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCV5526424.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            33042..34427
                     /gene="gspE"
                     /locus_tag="OVW45_001968"
     CDS             33042..34427
                     /gene="gspE"
                     /locus_tag="OVW45_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HCV5526425.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVNQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQKLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRQQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            34417..>34943
                     /locus_tag="OVW45_001969"
     CDS             34417..>34943
                     /locus_tag="OVW45_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HCV5526426.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMV"
BASE COUNT         8471 a         9455 c         8650 g         8367 t
ORIGIN      
        1 gtggaacttc tgtgactggt tctacggtaa aaatgggtga atatagtgca gaacaaatca
       61 gaaacggtgc cacaccggtt ccttttgata tttcacttca aaactgcgtt cgtgtgacta
      121 atattgagac aaaattagtt tcaacaaagg ttggtactga aaacgggcaa ctccttggta
      181 atactctcac aggtaatgac gcagcaaaag gagtcggtgt actcatagaa ggtttagcaa
      241 ctagtaaaaa tcctctaatg acattgaaac ctaatgattc aaattctgtt tataaagatt
      301 acgacccgag aggcaaagac gatacaacag gaggagttta cccggatcaa gatacaggta
      361 taacataccc tctccatttc caggccacgc tacaacagga tggaactata ccaatagaag
      421 ctggtgagtt taaagccacc agtactttcc aggtaaccta cccttaataa gtccaccgca
      481 ccgccatcat ctggcggtgc ggtaattgat aaatcattgc ccccaccaat gtgactgata
      541 aaatgccagg caacacgaca tcatttatca ggacacgtta tgaaaccgac caccattgct
      601 tcacttcaga aatgtaagca ggataaaaag cgtttcgcga ccatcaccgc ttacgactac
      661 agcttcgcga aactatttgc tgatgaagga cttaacgtca tgctggtagg cgactcgctg
      721 ggcatgacgg ttcagggtca cgactccacc ctgccggtga ccgttgccga tatcgcctac
      781 cacactgcct ccgtacgtcg tggtgcgcca aactgcctgc tgttggctga cctgccgttt
      841 atggcgtatg ccacgccgga acaagccttt gaaaacgccg caacggttat gcgtgccggt
      901 gccaatatgg tcaaaattga gggcggtgag tggctggtcg aaaccgtaaa aatgctgacc
      961 gaacgtgccg ttcctgtatg tggtcactta ggtttaaccc cacagtcagt gaatattttc
     1021 ggtggctaca aagttcaggg gcgcggcgat gaagcgggcg atcaactgct cagcgatgca
     1081 ttagccttag aagccgctgg ggcacagctg ctggtgctgg aatgcgtgcc gattgaactg
     1141 gcaaaacgta ttaccgaagc actggcgatc ccggttattg gcattggcgc aggcaacgtc
     1201 actgacgggc agatcctcgt gatgcacgac gccttcggca ttaccggcgg tcacattcct
     1261 aaattcgcta aaaatttcct cgccgaaacg ggcgacatcc gcgcggctgt gcggcagtat
     1321 atggctgaag tggagtccgg cgtttatccg ggcgaagaac acagtttcca ttaaggagtc
     1381 acgttgtgtt aattatcgaa accctgccgc tgctgcgtca gcaaattcgc cgcctgcgta
     1441 tggaaggcaa gcgcgtggcg ctggtgccta ctatgggtaa tctgcacgat ggccatatga
     1501 agctggtcga cgaagccaaa gcccgcgccg atgtggtcgt cgtcagtatt ttcgttaacc
     1561 cgatgcagtt cgaccgcccg gaagatctgg cacgttatcc acggaccttg caggaggact
     1621 gcgagaagct aaacaaacgt aaagtggatt tagttttcgc cccttcggta aaagagatct
     1681 acccgaacgg tactgaaacc catacttacg ttgacgttcc cgggctttcg accatgctgg
     1741 aaggcgcgag ccgtccgggg cattttcgcg gcgtttcgac tatcgtcagc aagctgttta
     1801 acctggtcca gccggacatc gcctgcttcg gtgaaaagga ctttcagcaa ctggcgctaa
     1861 tccgcaaaat ggttgccgat atgggcttcg atattgaaat tgtcggcgtc ccgattatgc
     1921 gcgccaaaga tggccttgcg ctcagttccc gtaatggtta tctgacggcg gaacaacgca
     1981 aaattgcgcc cggtctgtac aaagttttaa gttcaattgc cgacaaattg caggctggcg
     2041 agcgggatct tgatgaaatt attgccattg cagggcaaga actgaatgaa aaaggcttcc
     2101 gcgccgatga tattcagatt cgcgatgccg acacgttgct ggaagtctct gaaaccagca
     2161 aacgagctgt aattctggta gccgcctggc ttggcgatgc ccgcctgatc gacaacaaaa
     2221 tcgtcgaact ggcgtaatgc ttttactcta aataattcaa gttgcaggaa ggcggcaagc
     2281 gagtgacaaa ttcgtcagga acgaatttgc ccagccgcag gctggccttc ggtgagagac
     2341 atggatgtct ctcattaatc cccaggagct tacataagta agtgactggg gttagcgaac
     2401 gcagacgcag cacatgcaat ttaaagtatg acgagtataa ctggcgctac ggctgatggc
     2461 gccaattatt aatttgcccc acgtcatggc gatggcgtct gtaagcaatt attcataacg
     2521 cagacaactt agttaatcag cctatcaatc tcagcaagag ataaatttgt catctctgca
     2581 acgtcttccc gagacatccc tttactcaga aaacgctggg cgaattcttg cctttcctcc
     2641 tgtcttccct gctgaatccc cttctcaatc cctttctctt caaaccactg cgccagcgtc
     2701 atcatagact cccctcccgt ttccctgtct ctcagcacac cataaaacaa atccgcttgt
     2761 tcagtatgac cgcgttgcag catatagttt tgcatggcaa ctaactgact tccgctagtg
     2821 tacccttcgt caatcagcgt gaccagttgc tccagcaata acattaagtc gcgctggcga
     2881 atatgttttt gcagtagttc gagaatcgca atccgccgat gttgcatgat ttcgtcatcc
     2941 ggtgtgatgg tgatatccac cagcgggaaa ggactgttat agacgcgtcg tgccagctcc
     3001 ggcgagtaaa atatatcaaa ccagcacatt gagagcggat aaggtgtggc ctcgccctga
     3061 taaaacagta tcggcaccac cagcggcagc ttatcgtgat ccgcctccag atgccggtgc
     3121 atggcagcaa tagaataacg catcatgcga aagaccattt tcttatccgg cttgctttgg
     3181 tgttcaatca caacatgcag ataaccggga ttgccctgca tttgcacgga atagagcacg
     3241 tccgtgctgt gtcctttcag gctctcttca atgaaactcc ccgactctaa atgaagcgtg
     3301 ttgaggtcac aaagttctcg taattccact ggcaaatgta tctccagaaa gtcgcgagcc
     3361 gtctccgcgt gcattaaaaa ttgtttaaac actgcatcat gcggtgtggt actcggtgca
     3421 tccatcttcc ctgccttccg tgttgtcttg cgtgaaggca gtatccagtc aaggcgtgcc
     3481 atcgtcagcc acctctttag tgtttaccga gcagcgttca gagaaatttc aattggttac
     3541 agccgttaca tggcacgctt cggcacggta cgcaaactgc tgatgtaaat gccgtcagat
     3601 gagtagacac taaacaaaaa tcgggcaata ctgcgtgaga atttcctcaa agcaggcatc
     3661 gcctgcttcg ttaacgacag ggtagaaagg tagaagttat gattcgcacg atgctgcagg
     3721 gcaaactcca ccgcgtgaaa gtgactcatg cggacctgca ctatgaaggt tcttgcgcca
     3781 ttgaccagga ttttcttgac gcagccggta ttctcgaaaa cgaagccatt gatatctgga
     3841 atgtcaccaa cggcaagcgt ttctccactt atgccatcgc ggcagaacgc ggttcgagaa
     3901 ttatttctgt taacggtgcg gcggcccact gcgccagtgt cggcgatatt gtcatcatcg
     3961 ccagcttcgt taccatgcca gatgaagaag ctcgcacctg gcgacccaac gtcgcctatt
     4021 ttgaaggcga caatgaaatg aaacgtaccg cgaaagcgat tccggtacag gttgcttgat
     4081 tgttatccct gcggctggtt actcaccagc cgcgacatcg tctccagcga atccgttctt
     4141 aagatataaa gtcgttttag taacaacgga ttatcccccg gttttacttt gcctttcatg
     4201 gttgtcaccg ccaggtgaaa tccggcatcg tttgctgcct tcacggcttt gtcattaaat
     4261 ccgccaaacg gatacgaaag ataccagaca tgcggattaa attgcgccag agcgcggcgt
     4321 gaacgtgcaa aatcaaacag aatattgtgc tcactacggc tcagtaatat gggtcggcga
     4381 tagccatcca cccgatgcaa aaaatgggta tgtgactgaa aatcaaaaac atcgcgaatt
     4441 tcgttaagct cagaaacgct cataaattgc agcgattttg ggttccactt ctgcgggtga
     4501 cgtttgatgc gcgaggtaac aacaaacgcc gtcgccttca tgccatattg tttcaataca
     4561 ggatacgcat agcggctcac cgacttgagg ccatcatcaa aggtaatcac caccgctcgc
     4621 gcagggagat tgatcttatt cttcacgtaa ccttccagct gcgccatgct cagtgtcgcg
     4681 tatcccctgt cgcgcagcca ggccatttgg ttattgaaag cgcgtaccga ggtggtcgtc
     4741 gaagtatggc gaaaacgggt gttttcttcg tcgcgcagaa tatggtgata agttagcacc
     4801 ggaaggccat tatcgggttg ggcatccagc gcgctgatat aggccagtcg atcgccaata
     4861 cggacctgat accaggtttg atttaacctg tctttcagtt tatgcaaaat cgggtagcgc
     4921 aaattgtccg ccaatacccc aaatggcgca ctgcccacac tcggcgcgtt atagaccggc
     4981 gtatctttcc aggtaactaa gttctgatta ctcagcggct tgttgaggtc acccaaaccg
     5041 tcttcaactt tttgtcgccc ctgaaccggc tcgagatgcc ctttatcgat aaacccttta
     5101 ccaaagccaa aattaaatgc gtaataactt gcggcagtgg gctccacggc aatgatttgt
     5161 ccggcccgaa tattccccac ggtcaccatc ttgtcgccaa tttgcgccca gaccgcagca
     5221 ttttcgatgg tttgcatata acgggcaggt aacgcggcac tgacacttgc ggtaaacagc
     5281 atcagcagga gaataacagc ttgtttgtac atgaaaaaat aaccgagggt gaaacaacgc
     5341 ttttattgta gcaaaagcgc tataagaatc gtgggttaat actcatacaa attatgtttg
     5401 aggacgaaag gcggattttt ttgttgttgg tgccaaagac tactcacctc cggcccaccc
     5461 agttcgacaa tacgctcgcg aaactctgaa ctggtcatgg tttcacggca agccatcatc
     5521 tgttcaatta acggtaacgt gacaccagaa atcacttcgc aacgggagtg tttgtggctt
     5581 aataatgaag ccacgcggta cggtggcgct ccggctatat cggttaagaa gatgacaccc
     5641 tcaccagagt ccgcttcatg cagagcatcg cacatcatgc ggctgagcat attagagctt
     5701 aacccacgcc agaaattcac ggcccggcac tgaagaagcg ccccatgttt tttctccagc
     5761 gcatccagta tctcttgcgc ccggtcatcg tgacaggtaa ttacccaacc taacatcgcc
     5821 ttcctcctta gccgaaaatc agtctaccct gaccattgtt gataaagggt gatagccgtc
     5881 aaaactcctc tcccccaaat ccagaggaga ggaaatagcc ttagctacgc aaaccacgtc
     5941 cacgttggat cagcgaccaa cagatcaaat aaaacgccac aataaagacc accagtacgc
     6001 caaaggtagt gaccagcgga acatcattga taccgaggaa gccgtagcgg aaaccactga
     6061 tcatataaac gattgggttc aggtgcgaca gcccttgcca gaacggcggc aacaaagtca
     6121 gtgagtaaaa gaccccaccc aaatacgtga gtggcgttaa cacaaaggtt ggcaccaggc
     6181 tgatgtcatc gaacgttttg gcaaacacac cgttcagcaa acccgcaagg gagaacaaca
     6241 ccgccgtgag caccagggtt aaggcaacga atacccacga atgcacctga aacggcacaa
     6301 aaaacagtga aattgccgtc accagaatgc caacaaacag accacgcgcc acgccaccgc
     6361 cgacatatcc ggcaataatg acgtgagtcg gaaccggcgc taccagcagc tcttcaatat
     6421 tacgctggaa cttggcacca aaaaatgatg acgcaacgtt ggcgtaggca ttggtgatca
     6481 ccgacatcat gatcagcccc ggtacgatga actgcatata gctgaagcca tgcatatcgc
     6541 caatacgcga accaatcagg ttaccgaaga taataaagta aagggtcatg gtgatgactg
     6601 gcggcaccag cgtctgcacc cagatacgca taaagcgatg gatctctttc gcccagatgc
     6661 tttttagcgc cacccagtaa agatgcatca tgcgcgatct ccttgttttt cattaaccag
     6721 tgaaacaaac agctcttcca gacggttagc tttgttacgc atacttaata cctgaatgcc
     6781 ctgctcactt aactgcgtaa atacgctgtt gatcccctgc tcacgcagca cttcaacttc
     6841 cagcgtcgcg gtatcgacca gtcgatactg atagccatcg agcttcggta acggactttt
     6901 cggtgcgaga tcgagaataa aggtttccga tttcagcttc gccagcagcg ccttcatcga
     6961 ggtattttcc accagctcac cgtgttgaat aatgccgata ttgcggcaca gcatttctgc
     7021 ttcttccagg tagtgtgtgg tgagaatgat ggtggtgcct ttgtcgttta aatccttcaa
     7081 aaagccccac attgagcggc gaagttcaat atccacgcct gcggtcggtt cgtcgagaat
     7141 cagtagttta ggttcatgca ttaacgcacg ggcaatcatt aaacggcgct tcatcccgcc
     7201 agataacata cgcgcacgtt cgttgcgttt tccccataga tcgagttgtt taagatattt
     7261 ttcgctgcgg atgtacgcct ctttgcgctc cacaccgtag taccctgcct gattcaccac
     7321 aatttgctgc acggtttcaa acgggttgaa gttaaattcc tgcggcacca gtcccaactg
     7381 acgtttagcg ttcacgacat ctttctcgag atcgtaacca aatacgctga cccgcccgga
     7441 ggttttattt accagagagc tgataatacc gatagtggtc gatttcccgg ccccgttcgg
     7501 cccgagaagc gcataaaaat cacccgcttc gacctgcaaa tctattccac gaagcgcctg
     7561 aacgccgcct ggataggttt ttttaagctg ttgaagttcc agtgcaatgg tcataaattt
     7621 ttacttacct tacgttctta cactttatct gtggtttaaa tcgtcccgga gttgccctat
     7681 attagccaaa cgtaattatt tggttacagg tcgttaacct ccatgaaaga catagataca
     7741 ctcatcagca acaatgcact atggtcaaaa atgctggtgg aagaggatcc cgggtttttt
     7801 gagaaactgg cacaagcgca aaaaccgcgc tttctatgga ttggatgttc cgacagtcgc
     7861 gttcctgcag aacgtttaac cggtcttgag ccgggcgaac tctttgttca ccgtaatgtt
     7921 gctaacctgg tcattcacac cgacctgaac tgcctttccg tggttcagta tgcagtggat
     7981 gtactcgaag ttgaacacat tattatctgt ggccactacg gttgcggcgg cgtacaagcc
     8041 gccgttgaaa acccggaact ggggcttatc aacaactggc tgctgcatat ccgcgatatc
     8101 tggttcaaac atagctcatt gctcggcgaa atgccgcaag agcgccgtct ggataccttg
     8161 tgtgaactga acgtcatgga acaggtttat aacctaggcc actccaccat tatgcaatca
     8221 gcgtggaaac gcgggcagaa agttaccatt cacggctggg cctacggcat tcacgacggc
     8281 ttgctgcgtg atctggatgt cacagcgacc aaccgcgaaa cccttgagca acgttaccgt
     8341 cacgggattt ccaacctcaa gctgaaacac gccaaccaca aataaaaatg ccatgccgga
     8401 tgcaacacat ccggcaactt cacacttact cgtcccgcag aatcactttg ccgatatacg
     8461 gcagatgacg gtaacgctgt gcgtaatcaa tgccgtaacc caccacaaac tcatccggga
     8521 tcgagaaacc gataaattct accgggacgt tcacttcacg acgggacggt ttatccagca
     8581 gcgtacaaat cgccagcgac ttcggttcgc gcaggcttaa gatctcacgc actttcgaca
     8641 gtgtattccc cgagtcgatg atatcttcaa caatcagcac gtccttgcca cggatatctt
     8701 catccagatc tttgaggatt ttcacatcac gggtggtgga catgccgcta ccgtagctgg
     8761 aggcggtcat aaagtcgact tcatgagata cctgaacttc acggcacagg tccgccataa
     8821 acataaatga gccacgcagc agacccacca gcaccatatc gctgccgctg tctttgtaac
     8881 gctcagtaat ctgacgaccc agttcggcga tacgcgcttt aatctccgct tcggggatca
     8941 ttacttctac agtatgtttc atatctctaa ccatatgatt taaaaacaaa tcacttcgca
     9001 cgggcaggtt ttggcaccgc aacaaagcgc aagacacgca gtataccagt aaaactattc
     9061 attcgcggaa tctgttaata aagctcgaat tgtgatgacg gtcacacatg ttaaaaccta
     9121 taattaattg ctactaaaat attaatgaat tgaaatggtg tctctttatg gcaattaaca
     9181 atacaggctc gcgacgatta ctcgtcacgc taacagccct ttttgcagcg ctttgcgggc
     9241 tgtatctact cattggcgga ggctggctgg tcgcgattgg cggctcctgg tactacccta
     9301 tcgctggcct tgtgatgctc ggcgtcgcct ggatgctgtg gtgcagtaaa cgcgccgcgc
     9361 tttggctata cgcagccctg ctgctcggca ccatgatttg gggcgtctgg gaagttggtt
     9421 tcgacttctg ggcgctgact ccgcgcagcg atattctggt cttcttcggc atctggctga
     9481 tcctgccgtt tgtctggcgt cgcctggtca ttcctgccag cggcgcagtt gccgcactgg
     9541 tggtggcact gctgattagc ggtagtatcc tgacctgggc cggatttaac gatccgcagg
     9601 aaatcaacgg caccttaagc gccgatgcca cacctgcgga agctatctca cccgttgccg
     9661 atcaggactg gcctgcctat ggtcgcaatc aggaaggtca acgcttttca cctctgaaac
     9721 aaattaacgc cgataacgtc cataatctga aagaagcctg ggtgttccgt actggcgatg
     9781 tgaagcagcc gaacgatccg ggcgaaatca ccaatgaagt gacgccgatt aaagtgggcg
     9841 acacccttta cctgtgtacc gctcaccagc gcctgtttgc gctcgatgcc gccagcggca
     9901 aagagaaatg gcattacgat cctgagctga aaactaacga gtctttccag cacgtaacct
     9961 gccgtggcgt ctcttatcat gaagccaaag cagaaacggc ttcgccggaa gtgatggcgg
    10021 attgcccgcg tcgtatcatt cttccggtca atgatggtcg actgattgcg attaacgctg
    10081 aaaacggcaa actgtgcgaa accttcgcca ataaaggcgt gctcaatctg caaagcaata
    10141 tgccagacac caaaccgggt ctgtatgaac cgacttcgcc accgattatc accgataaaa
    10201 ccatcgtgat ggccggttca gttaccgata acttctcaac ccgcgaaacg tctggcgtga
    10261 tccgtggttt tgatgtcaac accggggagc tgctgtgggc ttttgatccc ggcgcgaaag
    10321 atccgaacgc aatcccgtct gacgaacaca cctttacctt taactcgcca aactcctggg
    10381 caccagcggc ctatgacgcg aagctggatc tggtctatct gccgatgggc gtgaccacgc
    10441 cggatatctg gggcggtaac cgcacaccgg aacaggaacg ttatgccagc tcgattctgg
    10501 cgctgaatgc cactaccggg aaactggcgt ggagctacca gaccgttcac cacgacctgt
    10561 gggacatgga tcttccggca cagccgacgc tggcggacat caccgttaat ggtcagaaag
    10621 tgccagttat ttacgctccg gcgaaaaccg gcaacatttt tgtgctcgat cgtcgcaatg
    10681 gcgaactggt ggttccggcc ccggaaaaac cggtgccgca aggtgctgcg aaaggcgatt
    10741 acgttactcc gacccagccg ttctctgaac tgagcttccg tccgaagaaa gatttgagcg
    10801 gtgcggatat gtggggagcc accatgtttg accaactggt gtgccgcgtg atgttccacc
    10861 agatgcgcta tgaaggcatt ttcacgccgc catctgaaca gggcacgctg gtcttcccgg
    10921 gtaacctggg aatgtttgaa tggggcggga tttccgttga tccaaatcgt gaagtggcaa
    10981 ttgccaaccc aatggcactg ccgtttgttt cgaaactgat cccacgcggt ccgggtaacc
    11041 cgatggagca gccgaaagat gccaaaggca cgggtacgga atccggcatt cagccacagt
    11101 acggtgtacc gtatggtgtc acgctcaacc cgttcctctc accgtttggt ctgccgtgta
    11161 aacagccagc atggggttat atctcggcgc tggatctgaa aatcaatgaa gtggtgtgga
    11221 agaaacgtat tggtacgccg caagacagta tgccgttccc gatgcctgta ccggtgccgt
    11281 tcaatatggg tatgccgatg ctgggcgggc caatctccac ggcgggtaac gtgctgttta
    11341 tcgctgctac ggcagataac tacctgcgcg cttacaacat gagcaacggt gaaaaactgt
    11401 ggcagggccg tttaccagcg ggtggtcagg cgacgccaat gacctatgaa gtgaatggca
    11461 agcagtatgt ggtgatctcc gcaggcggtc acggttcatt tggtacgaag atgggcgact
    11521 atattgtggc ttatgccctg ccggatgatg tgaaataacc ataatgcccg gagagatccg
    11581 ggcatatttc cgaatacggt ctttttatac cgtaaaccct aacatcatcc ccgtatcttc
    11641 atgctccagc agatggcagt gcgccatata agcatgttct ttcggtgcgt tgtgattaaa
    11701 cttcaccagc acttcactga cattgccttc taccttaacg gtatctttcc agcccgcgcg
    11761 atgagccgct ggcggtttgc cattttctga caagatacgg aactgcatgc cgtgaatatg
    11821 gaacggatgc agcatcatgt cgcccacgcc agagataacc caacgttcgt actgcccttt
    11881 cgccgccgca aacatcggct tattcatatc aaacgcctga ccgttgattt tattggcatg
    11941 gtggaaatcg aacttcccgc cgtggttcat atgattcata ttgccgtgcc ccatatggcc
    12001 catcatctgg ctgtgatcca ttccggccat cgcctgatcg ccatatttct ccattagcat
    12061 ctgcatcccc atcatatcga gcatcgggtc catagaaagt tgcagcttgc gtaccgtcag
    12121 cccttccagc gaaggtaacg caggcaggct acttaatgtg tcaggcaaag caccggaggc
    12181 actaatagca atcggctgaa tccgcattac cggatgcggc ttgtcaaacg gcgcaatcgc
    12241 catccccatc tggctgaccg gtagcgtcac cagatcaaag ggtttgttgt cgttaacctc
    12301 caccagcact tcaaaacgct cgcccatcag caccggcagc tcgctcacct tcaccggttc
    12361 aggtagcaga ccaccgtcgc tggcaatcac atacagcggg cgattgtcgc tggtggcgaa
    12421 attgagcgag cgggcgttac agccattgag caaacgcagg cgcagccagc cacgcggggc
    12481 agcgtgttgt gggtagattg caccgttggt cagcagcgta tcgccaaacc agcccacggc
    12541 ggcggtcatc acatccagtt gataatcaat ctgcccatca gcgttaaatt ttttatcctg
    12601 aacaatcacc ggaacatcat cgatacccca ctgttttggc agcattaatt tcaggatctc
    12661 gtcatcttca attaccacca gcccagccaa ccccatcgcc acctgtcgcc cggtcttgcc
    12721 atgttgatgt ggatggaacc agcaggtagc ggcaggttga tcaacgttca acgtcaccgt
    12781 acgtttgccg cctggcggaa taattccctg cgggccgccg tcgacgtcac ccggtacttc
    12841 cagcccgtgc cagtgcaacg tcgtctcttc cgccagttgg ttatagatat caaccgttac
    12901 cgctttgccg cgctgtaatt tcaccgccgg ccccagcaga ttgccgttat agccccaggt
    12961 agttgcagtt ttctcgccaa aggtagactg acctgcgcca atagttaact gaatgcgatt
    13021 acgggcatcg gtcgtgagca aatcagggat cggtaacgtt gggcgttccg ccgcaaagac
    13081 tgcgcggctc cacagcggca aggctgaagc cacacccagc gcgacggaat attttaagaa
    13141 atcacgacgt tgcatagtta tttccttatt cttaagcagg cgaaacaaaa tcaaacgttg
    13201 agcatagtcc ttccccttac gggaaggtca agccgcagac acaatttaat cgacaataat
    13261 ggtcgtcgcc tcgctcgcag tgtgctaacg tttatcttct ttaagcccct ggtagaagca
    13321 atgaagacgt ttttcagaac agtgttattc ggcagcctga tggccgtctg cgcaaacagt
    13381 tacgcgctca gcgagtctga agccgaagat atggccgatt taacggcagt ttttgtcttt
    13441 ctgaagaacg attgtggtta ccagaactta cctaacgggc aaattcgtcg cgcactggtc
    13501 tttttcgccc agcaaaacca gtgggatctc agtaattacg acaccttcga catgaaagcc
    13561 ctcggtgaag acagctaccg cgatctcagc ggcattggca ttcccgtcgc taaaaagtgc
    13621 aaagccctgg cccgcgattc cttaagcctg cttgcctacg tcaaataatc cctgatacct
    13681 ttttgtagaa atattgaccg tgcatccgcg gtcaatgtta gctattatgt tgcgcccttt
    13741 ttttacgggt gttaacaaag gaggtatcaa cccatggccg aaaaaaaaca gtggcatgaa
    13801 acgctacacg accagtttgg gcagtacttt gcggtagata acgttctgta tcatgaaaag
    13861 accgatcacc aagatctgat catttttgag aacgctgcat ttggtcgcgt aatggcgctg
    13921 gatggcgtag tacaaaccac cgagcgcgac gagtttatct atcatgagat gatgacccat
    13981 gttccgctac tggcccacgg tcacgcgaaa catgtgctga ttatcggcgg cggcgacggt
    14041 gccatgctgc gtgaagtaac ccgacataaa aacgttgagt caatcacgat ggtggaaatc
    14101 gatgcgggta tcgtgtcgtt ctgccgtcag tatctgccca accataacgc cggtagctac
    14161 gacgatccgc gctttaagct ggtgatcgac gatggcgtca atttcgttaa tcaaaccagc
    14221 cagacctttg atgtcattat ctccgactgc accgatccta tcggtcccgg cgaaagtctg
    14281 ttcacctcag cattttatga aggctgcaaa cgttgcctga atcctggcgg tatcttcgtc
    14341 gcacaaaacg gcgtctgctt tttacagcag gaagaagcca tcgacagcta tcgcaaactc
    14401 agccattact tcagcgacgt tggcttttat caggcggcga tcccgaccta ttacggcggt
    14461 atcatgactt ttgcatgggc gacagataac gacgccttac gccatctctc aaccgaaatt
    14521 attcaggcgc gttttctcgc ttctggcctg aaatgccgtt attacaatcc ggcaatccat
    14581 acggcagctt ttgccttacc tcagtatctg caagacgcac tggcttcaca gccgtcctaa
    14641 ggagaagata agaaattgaa aaaactgaaa ctgcatggct ttaataatct gaccaaaagt
    14701 ctgagttttt gtatttacga tatctgctac gccaaaactg ccgaagagcg cgacggttat
    14761 attgcttata tcgatgaact ctataatgcc aaccgtctga ccgaaatcct gtcagaaacc
    14821 tgttccatta tcggggccaa tattcttaac atcgcccgcc aggattacga accacagggt
    14881 gccagcgtca ctattctggt gagtgaagaa ccggttgacc cgaaactcat cgacaaaaca
    14941 gaacaccccg gcccactgcc agaaacggtc gttgcccatc tcgataaaag tcatatttgc
    15001 gtacatacct acccggaaag tcatcctgaa ggcggtttat gtaccttccg cgccgatatt
    15061 gaagtctcta cctgcggcgt gatttctccg ctgaaggcgc tgaattacct gatccaccag
    15121 cttgagtccg atatcgtaac cattgattat cgcgtgcgcg gttttacccg cgacattaac
    15181 ggtatgaagc actttatcga ccatgagatt aattcgattc agaactttat gtctgacgat
    15241 atgaaggcgc tgtatgacat ggtggatgtg aacgtctatc aggaaaatat cttccatacc
    15301 aagatgttgc ttaaagagtt cgaccttaag cactacatgt tccacaccaa accggaagat
    15361 ttaaccgaca gcgagcgcca ggaaattacc gctgcgctgt ggaaagaaat gcgcgagatt
    15421 tattacgggc gcaatatgcc agctgtttaa cggctctggc ggagctccca ggctccgcca
    15481 gatttattta cttctgctgc acgaaattgc ggtaagccgc cacgacctgc agaaaatcct
    15541 caacgccgca tagcgacagg ctttcttcgt cgtagtagtt catcccttct tccatttcat
    15601 cgccagcgaa ttccagttga ttggcgcgaa ccatcacctc ttcaccgtcc atccacaggg
    15661 tgtattcatg ccctgcccgt tgccaggacc gttcgctacc tttcagtgcg tgcgctgctt
    15721 gttccacttc atcaagcaaa gccaggtttt ctttcacctc ttcattaaac cagtgcccga
    15781 ccacttcatg ccccatggac atacgcacct ttaccactcc ggtaatatcg cgcagaaatt
    15841 cgtaatccat agtgtgttcc tctgctcccg gcaatgcgcc gaaatcgcat tgcgctattg
    15901 atgtaattat tgcagcctta ccggagaaaa acagcggaac ggcgatggtt tagaaataaa
    15961 aaatgcccgg gaatctcatt caccgggcat tgtgtcgttt atgcgcagcg cgtgcgctta
    16021 cttattacac cgcagtctgg aaaatcaccc catcggcttt ttcggtgtac tgagaaagct
    16081 ggttgaagtt cagataacga taagtatcaa cggcagtttt atctacttgc gcaacgtagg
    16141 tctggtactc ttccggcgtc ggcagtttgc caatcagcgc cgcaacagcc gccagttctg
    16201 cagaagccag gaagacattc gcgccagtcc ccagacggtt cgggaagtta cgggtagagg
    16261 tggaaaccac cgtcgcaccg tctgctacac gcgcctggtt acccatacac agagaacagc
    16321 ccgggatctc gatacgagcc ccgctcttac cgaagacgct gtagtagcct tcttcagtca
    16381 actgtgcggc gtccatgcgg gttggcggtg ccacccacag gcgggtcggc aactggcctt
    16441 tgtgcgcatc cagcagttta cccgcagcac ggaagtgacc gatgttagtc atgcaagaac
    16501 cgataaacac ttcgtcgatc ttctcgccct gtaccgcaga cagcggacgt gcgtcgtccg
    16561 ggtcgttcgg tgcacagagg attggctctt taatatccgc cagatcgatg tcgatcactg
    16621 ccgcgtattc agcatctgca tcggcttcca gcagctcagg attcgccagc catttttcca
    16681 tgccctgaat acgacgttcg agggtacgac gatcgccata accttccgcg atcatccact
    16741 tcagcaggac gatgttagag ttcaggtatt cgatgatcgg ttctttgttc agcttgatag
    16801 tacaaccagc ggcagaacgc tcagcggacg catcggtcag ttcaaatgcc tgctcaactt
    16861 tcagatccgg cagaccttca atttccagaa tgcgaccaga gaagatgttt ttcttgcctt
    16921 tcttctcaac ggtcagcaga ccttgtttga tcgcatacag cgggatcgcg tgtaccagat
    16981 cgcgcagggt gatgcccggc tgcattttgc ctttgaagcg caccagaacg gattccggca
    17041 tatccagcgg cattacgcct gttgcggctg caaatgccac cagaccagaa cccgccggga
    17101 aagagatacc gatcgggaaa cgggtatggg agtcaccacc ggtaccgacg gtatccggca
    17161 gcagcatacg gttcagccag gagtggatta cgccgtcacc cggacgcagc gacacaccac
    17221 cacggttcat aatgaagtcc ggcagcgtgt ggtgcgtgtt cacgtcaact ggcttcggat
    17281 acgccgcggt atggcagaaa gactgcatca ccaggtcagc cgagaagccc aggcacgcca
    17341 ggtctttcag ttcatcacgg gtcatcgggc cggtggtgtc ctgtgaacct acagaagtca
    17401 ttttcggttc gcagtacgcg cccggacgaa tacctttcac accgcaggca cgacctacca
    17461 ttttctgagc cagcgagaag ccgcgatcgc tctcagcgac atctttcgcc tgacggaaca
    17521 catcactgtg cggcagacca agtgcttcac gcgctttggt ggtcaggcca cgcccgataa
    17581 tcagcggaat acggccacca gcacgcactt catcaatcag cacgtcggtt ttcagttcga
    17641 aggtcgccag cagttcgccg gtttcgtggt tacgcacttc acctttgtac gggtaaacgt
    17701 caatcacgtc gcccatgttc aggttagaga cgtcgacttc gattggcagt gcacccgcgt
    17761 cttccatcgt gttaaagaag atgggtgcaa ttttaccgcc gaggcacaaa ccaccgccgc
    17821 gtttgttcgg cacatgtgga atatcatcgc ccataaacca cagaacggag ttcgtggcgg
    17881 atttacgcga tgaacccgta cccacaacgt caccgacgta cgccagcggg aaacctttct
    17941 gttgcagagc ttcgatttgc ttgatcgggc caacaacacc tggctggtct ggctcaatac
    18001 cttcacgggc gtttttcagc atcgccagcg cgtgcagtgg gatatccggg cgcgaccacg
    18061 catccggtgc cggagaaagg tcatcagtgt tggtttcgcc agtgactttg aagacggtaa
    18121 cggtcagttt ttcagccagc gccgggcgat tcaggaacca ttcggcatcc gcccaggact
    18181 gcattacctg cttcgcatat tcgttgcctg ctttggcttt ctcttctacg tcatagaagt
    18241 tatcgaacat cagcagtgtg tgagaaagtg ctttggcagc gatcggtgcc agtttggcat
    18301 catccagcgc gtcgatcagc ggatgaatgt tgtaaccacc ctgcatggta cccagcagtt
    18361 cgatggcttt ttccggagtc agcagagggg atttggcttc gcctttagcg atagcagcca
    18421 ggaagcctgc tttgacatag gcggcttcat cgacgccagg gggaacacgg ttggttaata
    18481 gatctaacag gaattcttct tcgcccgcgg gcgggttttt cagcagctct acaagtgcgg
    18541 ccatttggtt tgcatccagg ggtttgggcg caatcccctc agcggcacgc tcagctacgt
    18601 gcttacggta ttcttctagc acgacggttc tcctcgctct cattgtcata gtgcggcagg
    18661 cgattctctt cacgctcctg tgagacagca gtttgtaggg taaatgccca gtaccgcaac
    18721 gggcagaata gcaggatttt gacgacgtgt taatctgttt acaaaaaagc aacataaaaa
    18781 agtggctgaa tcgttaagga tggtctagag attgtttccc tccgtaaaaa cttcgttaat
    18841 tcgcatggtg ataatcacct ttctcaacga atcccaagat tagtcaaaat ttaaactacc
    18901 gcctctttat actcggatta acagcacctg cgggtggcag ttcgccgacc attgcgattt
    18961 ccttgagatc cgaattatga aaatgacttt gccgtttaaa ccccatgtgc tggcactaat
    19021 ttgcagtgcc gggctttgtg ccgcctctgc cgggctatat ataaaaagcc gcacagtgga
    19081 agcgcctgtg gaaacgcaat cgacacaaca gactgcgcct gacatcaccg cagttacgct
    19141 tcctgcaacg gtttccgcac cgcccgtaac gcccgccgtc gtcaaatccg cattcagcac
    19201 tgcacaaatc gatcaatggg ttgcgcctgt cgcgctgtac cccgattctc tgctttcaca
    19261 agtgttaatg gcatcaacct atccggcaaa cgttgctcaa gcagtgcaat ggtcgcacga
    19321 taatccactt aaacaaggcg atgctgctat tcaggcggta tctgaccagc cgtgggacgc
    19381 cagcgttaaa tcactggtgg cctttccaca attgatggca ttgatgggcg aaaacccgca
    19441 atgggtgcaa aacctgggcg atgcttttct ggcgcagccg caggacgtga tggactcggt
    19501 acagcgattg cggcaactgg cgcaacaaac cggctcgctg aagtcatcaa ccgaacagaa
    19561 agttattacc acaacgaaga aagctgtacc ggtaaaacag acagtcacgg cacccgtcat
    19621 accatccaat accgttttaa ctgccaaccc cgtcattaca gagccggcaa caaccgtcat
    19681 ttccattgag cccgccaatc ctgatgtggt ctatattccc aactacaacc caaccgtggt
    19741 ttacgggaac tgggccaata ctgcgtatcc gccggtttat ctgccaccac cagccggaga
    19801 accgtttgtt gacagctttg tgcgcggatt cggctatagc atgggcgttg ctaccacgta
    19861 cgcactattt agcagcatcg actgggatga cgacgatcat gaccatcatc atcatgacga
    19921 tgatgattat catcaccacg atggcggtca tcgtgacggt aatggctggc aacacaacgg
    19981 cgacaacatc aatatcgacg tcaacaattt caaccgtatc accggtgagc atcttactga
    20041 taagaatatg gcatggcggc acaatccaaa ctaccgtaat ggtgtgccct atcatgatca
    20101 ggatatggca aagcggtttc atcaaaccga tgtcaacggc ggaatgagtg ccacgcagct
    20161 acctgctcca acacgcgaca gccagcgtca ggcggcagca agtcagtttc agcaacgaac
    20221 acacgccgcc cccgtcatta cacgagatac ccaacgtcag gcagcggcac agcggtttaa
    20281 tgaagctgaa cactatggga gctatgacga cttccgcgac ttcagccgtc gccaaccact
    20341 gacccagcaa caaaaggacg ccgctcgtca gcgttatcaa tcggcctctc ctgagcagcg
    20401 ccaggcggtt cgcgagaaaa tgcagactaa cccacagaac cagcagcgaa gagaggcagc
    20461 gcgtgagcgt attcagtctg caactcccga acagcgccag gtgtttaagg aaaaagtaca
    20521 gcagcgccca ctgaaccaac agcaacgtga taacgcccgc cagcgtatac aatcggcatc
    20581 acctgaacaa cgtcaggttt ttcgggaaaa agttcaggag agccgcccac aacgtctaaa
    20641 cgacagtaac catactgtca ggctgaataa cgagcaacgg tcagcagtac gcgaacgtct
    20701 ctctgagcgc ggagcaaggc gacaggaaag gtaaattgca gacgtaaaaa aagcggcgtg
    20761 gttagccgct tttttaattg ccggatgttc cggcaaacga aaaattactt cttcttcgct
    20821 ttcgggttcg gcaggtcggt aatgctacct tcgaacactt ctgccgccag gcccacagac
    20881 tcgtgcagag tcgggtgcgc gtggatggtc agtgcgatgt cttcagcatc acaacccatt
    20941 tcgattgcca gaccgatttc acccagcagc tcaccgccgt tagtaccgac aatcgcacca
    21001 ccgatcacac ggtgagattc tttgtcgaaa atcagcttgg tcataccgtc tgcgcagtcg
    21061 gaagcgatag cacgaccaga agcagcccac gggaaggtgg cggtttcata gctgatgcct
    21121 ttctctttcg cttctttctc agtcagacct acccatgcaa cttctggttc ggtataggcg
    21181 atagacggga taactttcgg atcgaagtag tgttttttac cggcgataac ttcagcggca
    21241 acgtgacctt cgtgaacacc tttgtgtgcc agcatcggct gaccaacgat atcgccgata
    21301 gcaaagatgt gcggtacgtt ggtacgcagc tgtttgtcaa cgcggatgaa accacggtcg
    21361 tccacttcca cgccagcttt gcctgcgtcg aggtttttac cgttcggcac acgaccaatc
    21421 gctaccagca cggcgtcgta acgctgcggt tcagcgggtg cttttttgcc ttccatcgtc
    21481 acataaatac cgtcttcttt cgcttcaacg gcggtaactt tggtttccag catcaggttg
    21541 aatttcttgc tgatacgctt ggtgaagact ttaacgatgt ctttgtcagc tgccgggata
    21601 acctggtcga acatttcaac cacgtcaatc tgtgaaccca gcgcgtggta tacggtgccc
    21661 atttccagac cgatgatacc gccacccatt accagcaggc gttctggtac ttctttcagt
    21721 tccagcgcgt cagtggagtc ccagatacgc ggatcttcat gcggaataaa cggcagttgg
    21781 atcgggcgag aacccgctgc aatgatcgca ttgtcgaagt tgatcacggt tttgccgttc
    21841 tcaccttcaa cttccagggt gttagccccg gtgaatttac ccagaccgtt gaccactttg
    21901 actttgcggc ctttcgccat accagccaga ccaccggtca gctgattgat cactttctct
    21961 ttccaagtac gaatcttgtc aatatcggtt ttcggttcgc cgaagacgat accgtgttca
    22021 gccagcgctt tggcttcttc gataactttt gctacgtgca gcagtgcttt agaagggata
    22081 cagccgacgt tcaggcaaac accgccaagg gtgttgtaac gttctacgat tacggtttcc
    22141 agacctaaat cagcgcaacg gaaggcagcg gagtaacctg cggggcctgc cccaagtacc
    22201 acgacctgag ttttgatttc agtactcatc atgacctcta tatatttatc tccggcggtc
    22261 atacccgtcg tctttcaggc cgcagcggcg ttagcttcac gccctcaccc cagtcactta
    22321 cttatgtaag ctcctgggga ttctggcgct tgctaccttg ctgcacccag aaatccatag
    22381 ggtatgtgaa gtaataccct aaccaccacc gggtcgttct atccgtcggt attttacaaa
    22441 attgttaaca atttttaaac aacaaacggc aaccgatttg tctattcgct aataacctca
    22501 atacattcat gagattacca gaaaaaagcc ggccgttggg ccggctcttt tacttacatc
    22561 accagacggc gaatgtcaga cagcgtgttg ttaatgatgg taatgaaacg ggcaccatca
    22621 gcaccgtcga tcacgcggtg gtcgaaggag agagaaatcg gcagcatcag acgcggcacg
    22681 aactctttac cattccacac cggctccatc gcggacttgg aaacgccgag gatagccact
    22741 tccggcgcgt tcacaatcgg cgcgaagtgg gtagtaccca ggccgccgat gctggagatg
    22801 gtgaagcaac cgccctgcat ttcgcccgca gtcagcttac cgtcacgcgc tttcttagaa
    22861 atagtcatca gctcgcgaga cagctcgatg atgcctttct tgttgacgtc tttgaatacc
    22921 ggaacaacca gaccgttcgg ggtatccacc gccacaccga tgttgatgta tttcttcagg
    22981 gtcagacgct gaccgtcttc cgacagcgaa ctattgaagc gaggcatctg ctcaagagct
    23041 gcagcaacgg ctttcatgat gaagacaacc ggggtgagct tcacatccag cttacgtttc
    23101 gccgcttctt cgttctgctg tttacggaac gcttccagct cggtgatatc ggttttgtcg
    23161 aagtgggtaa catgcgggat catcacccag ttacggctca ggttagcacc agaaattttc
    23221 tggatacggc ccagttccac ttcttcgatt tcgccaaact tgctgaagtc caccttcggc
    23281 cacggcagca tgccagggat accaccgcca gtcgccgccg gagctgcttc tgcacgtttg
    23341 atagcttctt tcacgtaagc ctgaacgtct tcgcgcagga tacgaccttt acgaccagtg
    23401 cccttcactt tcgccaggtt aacgccaaac tcgcgtgcca gacggcggat cagcggagtc
    23461 gcgtgaacgt aagcgtcgtt ttcagcaaat tcagatttgc cttcggcttt cgcagccgga
    23521 gctgctgccg gggcttcagc tttcgctgcc ggagccggcg ctgccgcttc ctgtttcgca
    23581 ggagctgccg caggcgctgc gccttcaact tcgaagatca taatcagcga gccagttttc
    23641 actttatcgc caacgttgac tttcagttcc ttcacgacgc ctgcaaacgg agccgggacc
    23701 tccatagaag ctttatcgcc ttctacggtg ataagcgatt gttcagcggc aactttgtcg
    23761 cccactttca ccatcacttc ggtcacttca acttcgtcac cgccgatatc cggaacgtta
    23821 acttctttca cgccagccgc tgatgcaggg gccgctgccg gagctgcttc ctgtttagcc
    23881 gccggagctg ccgcgcctgc ttcacccgcg acttcgaaga ccataatcag cgagccggta
    23941 gacactttgt cacccacgtt cactttgatc tctttcacgg tgccagcaaa cggagccgga
    24001 acttccatag aagctttatc gccttctacg gtgatcagcg actgttcagc ttcaacttta
    24061 tcgccaactt tcaccaggat ttcggtcact tcaacttcgt cgctgccgat atccggaacg
    24121 ttaacgtctt ttgccgccgc agccgcgggt gctgctgccg gagctgcttc tttcttctct
    24181 tctgcctgag caggtgcagc gtctgctgca ccgtcggcgg aatcgaaaat cataatcagt
    24241 gcgccggtct gggttttatc gccaacagag actttgatct ctttaacgat acccgcctgc
    24301 ggagacggaa cttccataga ggctttgtcg ccttctacgg tgatcagcga ctgttcggct
    24361 tcaactttgt cgcccacttt gaccaggatc tcggtgattt caacttcatc agccccgatg
    24421 tccggtactt tgatttcgat agccattatt cttttacctc ttacgccaga cgcgggttaa
    24481 ctttatctgc atcgatgttg aatttggcga ttgcgtcagc aaccactttc ttatcgattt
    24541 cgccacgttt agccagttcg cccagcgccg caaccacgac ataagaagca tcaacttcga
    24601 agtggtgacg caggttctca cggctgtcgg aacgaccgaa gccatcagta cccagtacgc
    24661 ggtagtcgtc agccggtacg taagtacgga cctgctcagc gaacagtttc atatagtcgg
    24721 tagatgccac tgccggagcg tcgttcatca cctgagcgat atacggaacg cgcggagttt
    24781 ccagcggatg aagcatgttc cagcgttcac aatcctgacc atcacgcgcc agctcggtga
    24841 aggaggtcac gctataaacg tcagaaccta cgccgtaatc tttcgccagg atctctgccg
    24901 cttcacggac gtgacgcagg atagaaccgg agcccagcag ctgaacttta cctttgctac
    24961 cttcaatggt ttcgagtttg tagatacctt tacggatacc ttcctcagca ccttccggca
    25021 ttgccggcat gtggtagttt tcgttcagcg tagtgatgta gtagtaaacg ttctcttgtt
    25081 tttcaccgta catacgctcc agaccgtcat gcatgatgac agcaacttcg taagcgtaag
    25141 ccgggtcgta agagatacag ttcgggatag tcagcgactg aatgtggctg tgaccatctt
    25201 cgtgctgcag accttcaccg ttcagggtgg tacgaccgga agtaccgccg atcaggaagc
    25261 cacgcgcttg ctggtcgcca gccgcccagc acagatcgcc gatacgctgg aagccgaaca
    25321 tcgagtaata gatgtagaac ggaatcatcg gcagattgtt ggtgctgtaa gaggtcgccg
    25381 ctgccagcca ggaacaccct gcgcccagct cgttgatccc ttcctgcaga atctgacctt
    25441 tctcgtcttc tttatagtaa gcaacctgct cgcggtcctg cgggatgtac tgctgaccgt
    25501 tcgggctgta aataccaatc tgacggaaca gaccttccat accgaaagta cgcgcttcgt
    25561 cggcgatgat cggtaccaga cgatctttga tcgacttgtt cttcagcatt acgttcaggg
    25621 cacgaacgaa agcgatagtg gtagagatct ctttgctctg ctcttccagc agcgcgccga
    25681 agtcttgcag gctcggcagc tcaagcttct cggtgaagtt cggctgacgg cttggcagat
    25741 aaccgtgcag tttctgacgc tgtgcgtgca gataggtatg ctcttcagaa ccttccggga
    25801 aggtgatgta cggcagtttt tcgatatctg catcagacac cggcacattg aaacggtcgc
    25861 ggatgtgacg cacgccgtcc atgttcattt tcttaacctg gtgcgcgatg tttttacctt
    25921 cagccgcgtc gcccatgccg taacctttaa tggtatgagc aaggattact gtcgctttgc
    25981 ctttggtttc ctgcgctttc ttgaatgcag cgtagatttt cttcggatcg tgaccaccac
    26041 ggttcagtgc ccagatctgc tcgtcagtcc agtctgcaac cagtgctgcg gtttcaggat
    26101 atttaccgaa gaagtgttca cgaacgtacg caccatcttt cgatttgaag gtctggtagt
    26161 cgccgtcaac ggtttcgttc atcagctgga tcagtttacc gctggtatct ttacgcagca
    26221 gttcatccca acggctaccc cacatcactt tgataacgtt ccagccagca ccttcgaaga
    26281 tgccttccag ttcgttgatg atcttgccgt taccggtgac cgggccgtca agacgctgca
    26341 ggttacagtt gataacgaag accaggttat ccagtttttc acgggtagcg atggtgatcg
    26401 cacctttgga ttccggttcg tccatttcac cgtcgccgag gaacgcgtaa acggtctgtt
    26461 tagaggtatc tttcaggcca cggtgttcca gatatttcag gaatttagcc tggtaaatag
    26521 caccaatcgg ccccagaccc atagatacgg tcgggaactg ccagaattcc ggcatcagtt
    26581 tcgggtgcgg ataggaagag aggccattgc cgtgaacttc ctgacggaag ttatccagct
    26641 gctcttgagt cagacgacct tccaggaaag cacgagcgta cacgcccggg gagatgtggc
    26701 cctggaagta aaccaggtcg ccgccatcct gctcgttgcg tgcacggaag aagtggttaa
    26761 agcacacatc ataaatggtt gcggaagact ggaaggacgc catatggccg cccagttcga
    26821 ggtctttttt cgacgcacgc agcaccgtca tgatggcgtt ccagcggata gctgaacgaa
    26881 tacggcgttc cagttccaga ttacccggat actccggttg ttcttcaacg gggatggtgt
    26941 tgatgtagtt gctgatacct gtgcctgcgg ctacgtttac accgcctttg cgggcttcag
    27001 caagcagttg gtcgatcaga tactgagcac gctcaacacc ttcttcacgg atgaccgatt
    27061 cgatcgcctg gagccagtcg cgagtttcga tcggatccac gtcatttggg aaacgttctg
    27121 acatgggtta ttccttatct atctaataac gttgagtttt ctggaacctg tcccattgaa
    27181 ctctcgccgg aaagctcaat aagacaggtt ctacgtttag ttgccgcgct tttatatacg
    27241 cttgatttac aacatcttct ggataatttt taccagaaaa atcactaatt ctttcgttgc
    27301 tccagacgac gcagagaacg ctcacggcgg ctctcttcac gacttctgtc gagcaaaatt
    27361 tcttcgataa aggccagatg gcgatgcgat gcttcgcgcg cttcttccgg cttaccggcc
    27421 ataatcgctt caaatatgcg ggtgcggtga ctactcacca gcggcagcat ctcgcgacgc
    27481 gaatagagca attcgaagtt ctggcggaca ttctgggcca acatcggctc catacacctt
    27541 agcagatgaa gcagaaccac attgtgggcc gcttcggtga cggcaatctg atactggagt
    27601 acggcgtttg attccgcgtc cagatcgcca gactgctgcg ccagctctat ggcgtggtgg
    27661 agttcacgga tgcgttcctt gtcttcatcg gtactacgca gcgcggcgta ataagcggcg
    27721 ataccttcca gggcgtgtcg tgtttcgagc aagtcatact gtgattcagg atggtcggag
    27781 agcagctcca ccagcggatc gctgaagctt tgccataggc tgctctggac aaaagtgccg
    27841 ccaccctggc gacgaagcaa caagcccttc gcttcgagac gttgaatcgc ctcacgcaag
    27901 gagggacggg agacgtcgaa ctgttttgcc agttcgcgtt ccggtgggag tttttcgccc
    27961 gggcggagag tgccttcgag aatcaaaaac tccagttgct gctcaatcac atcggagagt
    28021 tttggttggc ggattttgct gtaggccatg agttcctgtc ttaagccact tgccgaagtc
    28081 aattggtctt accaatttca tgtctgtgac gctaaagtaa caaagtattc accttatgtc
    28141 catacaggtt ttgattgaaa tcatgaaact gtgcacattt taacaacttg acatatataa
    28201 cgtttcaaaa ttgtaactat gcacaaatgt agactttacg taggaaaggg attttttaac
    28261 gattattaaa ctataaaaat ccgaattgaa ccgattcact taccaatttt ttgattttta
    28321 agtcaataaa aacgaattta aattcattct gcatattgag aggggttgag gctgagcttt
    28381 acaaacggtt tctttttaag caactcatct tcaaccatgc ataaagcggg tgcattcgct
    28441 gccgcatacc attattcttg atctgacgga agtctttttg taacaattca aacttctttg
    28501 atgtaaaaaa attaatacaa caaacggaat tgcaaactta cacacgcatc actgcgtaga
    28561 tcaaaaaaac aatcaccgca cgaggtttca tgatggaagg tcaacagcac ggcgagcagc
    28621 taaagcgcgg ccttaaaaac cgccatattc agcttatcgc gctgggtggc gcgataggga
    28681 cagggctatt cctgggtagc gcctccgtaa tacagtccgc agggccaggg attatcctgg
    28741 gttacgccat tgctggtttt atcgcctttc tgatcatgcg tcagctgggt gaaatggtgg
    28801 tcgaagaacc tgtcgcaggc tcctttagcc actttgctta taaatactgg ggcagttttg
    28861 ctggtttcgc ctctggctgg aactactggg tactgtacgt tttagttgcc atggcagagc
    28921 tgactgccgt gggtaaatac attcagttct ggtatccgga aatcccaacc tgggtttctg
    28981 ccgccgtatt ctttgtggtg attaacgcca tcaacctgac caacgttaaa gtgtttggcg
    29041 agatggagtt ctggtttgcc attatcaaag ttattgcggt agtagcgatg atcatcttcg
    29101 gcggctggct gctgttcagt ggcaacggcg gcccgcaggc gagcgttagc aacctgtggg
    29161 atcaaggtgg tttcctgccg cacggcttca ccggactggt gatgatgatg gcgattatca
    29221 tgttctcgtt cggtggtctg gaactggtgg ggatcaccgc agcagaagct gataacccgg
    29281 agcaaagtat cccgaaagcg actaaccagg ttatctaccg catcctgatt ttctatattg
    29341 gttcgttagc cgttctgctc tcactgatgc cgtggacccg cgttaccgcc gataccagtc
    29401 cgtttgtgct gatcttccac gagttaggcg atacctttgt ggcgaatgcg ctgaacatcg
    29461 tggtactgac tgcggcgctc tccgtgtaca acagctgcgt atattgcaac agccgtatgc
    29521 tgtttggtct ggcacaacag ggtaatgcgc caaaagcgct ggcgtctgtc gataaacgcg
    29581 gcgtaccggt taacaccatt ctggtgtctg cgctggttac agcattgtgc gtattgatta
    29641 actatcttgc tccggaatcc gcatttggcc tgttaatggc actggtggta tccgcactgg
    29701 tgatcaactg ggcgatgatc agtctggcgc atatgaagtt ccgtcgcgcc aagcaggaac
    29761 aaggcgtggt aactcgcttc cctgctctgc tttatccgct gggtaactgg atctgcctgc
    29821 tgtttatggc ggcggtactg gtgattatgc tgatgacccc aggaatggcg atttcggtat
    29881 acctgatccc ggtatggctg atcgtgttag gtatcggcta tctgtttaaa gagaaaaccg
    29941 ccaaagccgt aaaagcgcat taatctctct acgccctcac ccatgcaggg tgagggcaat
    30001 aatctttaca ccaacgctcc gtaaatcgtc agtagcgcaa tcaccaccac gaccacgaac
    30061 gaggttttct tcgccattga aaccgctgcc ttcggcgtct ccaccttatc gacatgcggt
    30121 tcacgcgcca gagagaactg cgccagacgc gttaacacct gatactgcga agtatggaag
    30181 tcacccagcg aagcaaacca ggccggtaac gctttctccc catgaccgat caaggcatat
    30241 accacacccg caagacgaac cggcacccaa tccagtacat gaagcacggc atcaatgccg
    30301 gactgtaaac gatgatgcgg cgtctggtat cgcgccagcc agtattgcca tgcacgcaaa
    30361 aacgcatacc ccatcagcgt aacgggtccc caggttcccc ccacaatcaa ccagaacagc
    30421 ggtgcaagat aaaaacgaaa gttaatccac agcaatgcat tttgcagctc acgcaaatac
    30481 tcacgttcgt cgcagcctgc cgggacgccg tgaatcatgg tgagttcgcc agccatcgtg
    30541 gcacgggcat ggctatcatt acgtgaagca gctgtcagat aagcatgata atgaagacga
    30601 actttacctg cgccaataca cagcaaacca atcagcagcc acaccagcag cgtgggaacg
    30661 ttgaacaata ctccctgcaa tgcgcgtaac agtaaaaaag tcacgcccat cgcaataatg
    30721 gtcatgccta acgtgcgccc gagagaaaaa tgtttcaccc gccgaaagaa cgcttcaaga
    30781 cgatgatcaa gctgccagtg ctcgcccaac ttaaacaggc gctcgaaaat taacaccagt
    30841 aaggttgtaa atagcgtcat gttgtctcct tgctgaccag cgcacgaaac cgtgcccaat
    30901 caaatgcagg accgggatcg gttttccgat ccggcgcaat atcacaatgg cccgtcatgt
    30961 tattagcgat atcgggatag cgatctatca gtgcccgcgt aaccgccgca agctgttgat
    31021 actgcgcatc ggtatacgcc agagtatcgg tgccttcaag ctcaatccca atagaaaaat
    31081 cattgcagcg ttcacgcccc tgatactgag agactcccgc atgccaggca cgtttatcga
    31141 aaggaacata ctggactatt tcaccatcac ggcgaatcaa acagtgagcg gagacgcgca
    31201 aatgggcgat ctcagcaaag aaaggatgtg cctgcggatc aatagttcca gtgaataatg
    31261 cgtcgatcca cggaccgcca aactcgcctg gcggcaggct aatattgtgc accaccagca
    31321 gggtgggtgt ttcgtcatcc gggcggcaat cgtaatgtgg tgagggaacg cggcgcgcgc
    31381 caaccagcca cccctgttct aacaacatgc aggatctcct tatatgtggt gctaataccc
    31441 ggttcagagt agcatgtttc taccttatga ttcgttagct atctggagtt ttaacatgcc
    31501 gcctcgccgc tataaccctg acacccgacg tgacgagctg ctggaacgca ttaatctcga
    31561 tatccccggc gcggtggccc aggcgctgcg ggaagattta ggcggaacag tcgatgccaa
    31621 caatgacatt acggcaaaac ttttaccgga aaactctcgc tctcatgcca cggtgagcac
    31681 ccgcgagaat ggcgtatttt gcggcaaacg ctgggttgaa gaggtgttta ttcaactggc
    31741 aggcgacgat gtcaccataa tctggcatgt ggatgacggc gatgtcatca acgccaatca
    31801 acccttgttc gaacttgaag gcccatcccg cgtgctgtta acgggcgaac gcactgcgct
    31861 taattttgtg caaacccttt caggagttgc cagtaaggta cgccactatg tcgaattact
    31921 ggaaggcacc aacacgcagt tgttggatac gcgcaaaacc ttacctggcc tgcgttcagc
    31981 tctgaaatac gcggtacttt gcggcggcgg agcgaatcac cgtctggggc tttctgatgc
    32041 cttcctgatc aaagaaaatc atattattgc ctccggctca gtgcgccagg ccgtcgaaaa
    32101 agcgtcctgg ctgcacccgg atgcgccagt agaagtcgaa gtagagaatc tggaagaact
    32161 tgatgaagcc ctgaaagcag gagccgatat catcatgctg gataacttcg aaacagaaca
    32221 gatgcgcgaa gccgtcaaac gcaccaacgg caaggcgcta ctggaagtgt ctggcaacgt
    32281 cactgacaaa acactgcgcg aatttgccga aacgggcgtg gactttatct ccgtcggtgc
    32341 gctaactaaa cacgtacaag cactcgacct ttcaatgcgt tttcgctaat tgctgacaga
    32401 tttatccgct ccatcgcggg cggataatct ccccttcccc aactttcttc gtaacgcctc
    32461 gcaaatttct tgtcttttgc tgcaacgaaa taaaaaacct cggaagctgc tttcccgttt
    32521 cgttttttgc ccctcgcttg tgctgatggc gtttgccaaa gtagcgccaa ccaaatcaag
    32581 gagcgaaaca gatggacaag caacgcggtt ttacacttat cgaactgatg gtggttattg
    32641 gcatcattgc cattttaagc gccattggta ttcccgctta tcaaaactac ctgcgcaaag
    32701 ctgcactcac cgacatgcta caaacctttg tgccttaccg taccgccgta gagttgtgcg
    32761 cgctggaaca tggtggatta gatacctgcg acggtggcag caatggcatt ccctcgccta
    32821 ccaccacccg ctatgtttca gccatgagtg tggcaaaagg cgtggtgtcg ctgaccgggc
    32881 aagaaagtct caatggacta agcgtcgtca tgacaccggg ttgggataac gcaaacggcg
    32941 tcaccggctg ggcgcgcaac tgcaatattc aaagtgacag cgcattgcag caagcctgcg
    33001 aagatgtctt ccgctttgat gacgccaact aaggagcggc aatgaatatt ccacagctca
    33061 ccgccctgtg tctgcgttat cagggagtct tgctggatgc cagcgaagag gtggttcatg
    33121 ttgcggtagt cgatgcacct tcgcatgagt tgctggacgc attgcatttc gctaccacca
    33181 aacgtattga gatcacctgc tggacgcgcc aacaaatgga aggccacgcc agtcgcacac
    33241 aacagacatt gcccgtagct gttcaggaga agcatcagcc caaagcagag ttgctgactc
    33301 gaacgttaca atctgcgctg gaacaacgtg cgtctgatat tcatatcgaa ccagcggaca
    33361 atgcctaccg catccgcttg cgtatcgacg gcgtattgca tcctttaccg gatgtttcac
    33421 cggatgccgg agtcgcatta accgccagat taaaagtgct gggaaacctg gatattgcgg
    33481 aacatcgcct gccgcaggac gggcaattca ctgtcgaact ggcaggaaac gccgtctcat
    33541 ttcgtattgc gaccttacca tgtcggggtg gtgaaaaggt ggtattaagg ttgttacagc
    33601 aggtgaacca ggcactggat gttaacacgc tgggaatgca gccgttacaa ctggcggact
    33661 ttgctcatgc cttgcaacaa ccacagggac tggtgctggt aactggccct acaggcagcg
    33721 gcaaaacggt cacgctttat agtgccctgc aaaagctgaa taccgctgac attaatattt
    33781 gtagcgtcga agatccggtt gagatcccca tagccggact aaaccagacg caaatccatc
    33841 cgcgtgccgg gctcaccttt cagggcgttt tgcgtgcgtt attgcgccag gatcctgacg
    33901 tcatcatgat cggagagatc cgcgatggcg aaacggcaga aattgccatt aaagccgcgc
    33961 aaaccggtca cctggtgttg tctaccctac acactaattc cacctgcgaa acgctggtac
    34021 gtttacagca aatgggagtc gcccgctgga tgctctcatc agcgcttacg ctggtaatag
    34081 cccagcgtct ggtacgtaaa ctttgcccac attgtcgcca gcagcaaggg gagcccatcc
    34141 atattccagt caatgtatgg ccgtcgccgc tgccccactg gcaggcaccc ggttgtgtac
    34201 attgctacca cggtttttat ggtcgtacgg ccttatttga agttctgccc ataacgccgg
    34261 tcattcgtca gcttatttcc gctaataccg acgttgaatc gctggaaacg cacgcacgac
    34321 aggcgggtat gcgtacgctt tttgaaaacg gctgcctggc cgtggagcaa ggcttaacca
    34381 cctttgaaga gttaatccgc gtactgggga tgccgcatgg cgagtaagca actctggcgc
    34441 tggcatggca taaccggcga cggcaatgcg caagatggga tgctatgggc agagagccgt
    34501 gctttgctgc tcatggcact acagcaacag atggttaccc cacttagcct gaagcgaatc
    34561 gccatcaatt ctgcgcagtg gcgaggagat aaaagcgcgg aagtcattca tcaactggcg
    34621 acgctactca aagccgggtt aacgctttct gaagggctgg cactgctggc ggaacagcat
    34681 cccagtaagc aatggcaagc gttgctgcaa tcgctggcgc acgatctcga acagggcatt
    34741 gctttttcca atgccttatt accctggtca gaggtatttc cgccactcta tcaggcgatg
    34801 atccgcacgg gtgaactgac cggtaagctg gatgaatgct gctttgaact ggcgcgtcag
    34861 caaaaagccc agcgtcagtt gaccgacaaa gtgaaatcag cgttacgtta tcccatcatc
    34921 attttagcga tggcaatcat ggt
//
LOCUS       DAKISA010000040        32926 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.040, whole genome shotgun sequence.
ACCESSION   DAKISA010000040 DAKISA010000000
VERSION     DAKISA010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32926
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.040"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            93..224
                     /locus_tag="OVW45_001970"
     CDS             93..224
                     /locus_tag="OVW45_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HCV5526427.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVERMGSWPEVVMA"
     gene            complement(484..1131)
                     /gene="yaiW"
                     /locus_tag="OVW45_001971"
                     /pseudo
     CDS             complement(484..1131)
                     /gene="yaiW"
                     /locus_tag="OVW45_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
     gene            complement(1144..2364)
                     /gene="sbmA"
                     /locus_tag="OVW45_001972"
     CDS             complement(1144..2364)
                     /gene="sbmA"
                     /locus_tag="OVW45_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCV5526428.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            2716..3873
                     /gene="ampH"
                     /locus_tag="OVW45_001973"
     CDS             2716..3873
                     /gene="ampH"
                     /locus_tag="OVW45_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCV5526429.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYIPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(3874..4497)
                     /gene="iprA"
                     /locus_tag="OVW45_001974"
     CDS             complement(3874..4497)
                     /gene="iprA"
                     /locus_tag="OVW45_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HCV5526430.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(4585..7473)
                     /locus_tag="OVW45_001975"
     CDS             complement(4585..7473)
                     /locus_tag="OVW45_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCV5526431.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATYNTFGYHDDAATIYN
                     WGDSTKIDHDYLTYGGYVYDHAADGYFDTTFSGDTVNGVISTYYLNHDYGTDTGNTLN
                     ISNSIVHGMVTSYQVGYGDYVWTNGSDYTGHDWVDGDIFTLNIANSTIDDDFDAFYFT
                     DTYLDADGKTSKTDYDRLVTAGLGTAVTLDVESNINISNNSHVAGITLVQHDLGNATY
                     NTEGHQWDNNIVVNDSTVTSGSWTEDEQSDRGHFGNSVEPSDYGNGASPADDVALAFI
                     DDDTSDYRMVNNVTFNNSQLLGDVVFDSTWNANFDATGHLIDNSTTAYTHGGWATDDQ
                     NVDHLNLTLNNTKWVGSANIDYDVVVADEAFYDVAPNSLNPYASYSEDGWNRVDNANA
                     FQSGVFDVVLNNGSDWETTKTSLIDTLAINSGSQVNVSADSSLTSDTITLNGGSSMEV
                     NGEVNTDHLTIDTFSTVNFGEDTASAWAYAPLYANTITVTNGGVLDVNTNMNDISSVF
                     ATDTLELTSGNVKDNNGNVYAGVFDIHSNDYILNADLVNDRTNDTSKANYGYGVIAMN
                     SDGHLTVNGNNDINNGDEVDNSSADNVVAATGNYKVRIDNSTGAGAIADYAGKQLIYI
                     DDTKTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNL
                     EQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKVDGN
                     NAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDL
                     SATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSVSGLFQSGDDYQLSND
                     MKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNG
                     TEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
     gene            7998..8972
                     /gene="hemB"
                     /locus_tag="OVW45_001976"
     CDS             7998..8972
                     /gene="hemB"
                     /locus_tag="OVW45_001976"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCV5526432.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(9078..9929)
                     /gene="tauD"
                     /locus_tag="OVW45_001977"
     CDS             complement(9078..9929)
                     /gene="tauD"
                     /locus_tag="OVW45_001977"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCV5526433.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(9926..10753)
                     /gene="tauC"
                     /locus_tag="OVW45_001978"
     CDS             complement(9926..10753)
                     /gene="tauC"
                     /locus_tag="OVW45_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCV5526434.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     VLAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(10750..11517)
                     /gene="tauB"
                     /locus_tag="OVW45_001979"
     CDS             complement(10750..11517)
                     /gene="tauB"
                     /locus_tag="OVW45_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCV5526435.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(11530..12492)
                     /gene="tauA"
                     /locus_tag="OVW45_001980"
     CDS             complement(11530..12492)
                     /gene="tauA"
                     /locus_tag="OVW45_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCV5526436.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            13102..13773
                     /locus_tag="OVW45_001981"
     CDS             13102..13773
                     /locus_tag="OVW45_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HCV5526437.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            13783..14979
                     /locus_tag="OVW45_001982"
     CDS             13783..14979
                     /locus_tag="OVW45_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCV5526438.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIKRGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVIYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            14979..15539
                     /locus_tag="OVW45_001983"
     CDS             14979..15539
                     /locus_tag="OVW45_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCV5526439.1"
                     /translation="MLKRKIIQIADQVIIDETAGEVVIGANTRICHGAVIQGPVVIGA
                     NCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCYIADSVVANQAYLGA
                     QVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQL
                     GPRVIVERNLPTGTYSLRQELIRTGD"
     gene            15541..16314
                     /locus_tag="OVW45_001984"
     CDS             15541..16314
                     /locus_tag="OVW45_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HCV5526440.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            16502..16777
                     /gene="frmR"
                     /locus_tag="OVW45_001985"
     CDS             16502..16777
                     /gene="frmR"
                     /locus_tag="OVW45_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HCV5526441.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            16812..17921
                     /gene="frmA"
                     /locus_tag="OVW45_001986"
     CDS             16812..17921
                     /gene="frmA"
                     /locus_tag="OVW45_001986"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HCV5526442.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            18014..18847
                     /gene="fghA"
                     /locus_tag="OVW45_001987"
     CDS             18014..18847
                     /gene="fghA"
                     /locus_tag="OVW45_001987"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCV5526443.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(19071..19610)
                     /locus_tag="OVW45_001988"
     CDS             complement(19071..19610)
                     /locus_tag="OVW45_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HCV5526444.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYTIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(19712..20923)
                     /gene="mhpT"
                     /locus_tag="OVW45_001989"
     CDS             complement(19712..20923)
                     /gene="mhpT"
                     /locus_tag="OVW45_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HCV5526445.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(21109..22122)
                     /gene="dmpG"
                     /locus_tag="OVW45_001990"
     CDS             complement(21109..22122)
                     /gene="dmpG"
                     /locus_tag="OVW45_001990"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HCV5526446.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVRHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(22119..23069)
                     /gene="mhpF"
                     /locus_tag="OVW45_001991"
     CDS             complement(22119..23069)
                     /gene="mhpF"
                     /locus_tag="OVW45_001991"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HCV5526447.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(23066..23875)
                     /gene="mhpD"
                     /locus_tag="OVW45_001992"
     CDS             complement(23066..23875)
                     /gene="mhpD"
                     /locus_tag="OVW45_001992"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HCV5526448.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDHAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(23885..24751)
                     /gene="mhpC"
                     /locus_tag="OVW45_001993"
     CDS             complement(23885..24751)
                     /gene="mhpC"
                     /locus_tag="OVW45_001993"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HCV5526449.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(24769..25713)
                     /gene="mhpB"
                     /locus_tag="OVW45_001994"
     CDS             complement(24769..25713)
                     /gene="mhpB"
                     /locus_tag="OVW45_001994"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HCV5526450.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(25715..27379)
                     /locus_tag="OVW45_001995"
     CDS             complement(25715..27379)
                     /locus_tag="OVW45_001995"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HCV5526451.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETIKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            27421..28254
                     /gene="mhpR"
                     /locus_tag="OVW45_001996"
                     /pseudo
     CDS             27421..28254
                     /gene="mhpR"
                     /locus_tag="OVW45_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator MhpR"
     gene            28331..29413
                     /gene="lacI"
                     /locus_tag="OVW45_001997"
     CDS             28331..29413
                     /gene="lacI"
                     /locus_tag="OVW45_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HCV5526452.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            29536..32610
                     /gene="lacZ"
                     /locus_tag="OVW45_001998"
     CDS             29536..32610
                     /gene="lacZ"
                     /locus_tag="OVW45_001998"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HCV5526453.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNIDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGKNRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PSTQISDFHVATHFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPALWSAEIPNLYRAVVELHTDDGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRFGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWSVALDGKPLASGEMPLDVAPQDKQLIE
                     LPELPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAAPHAIPQ
                     LTTSETDFCIELGNKRWQFNRQSGFLSQMWIGAEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGSYHYQLVWCQK"
     gene            32662..>32926
                     /locus_tag="OVW45_001999"
     CDS             32662..>32926
                     /locus_tag="OVW45_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCV5526454.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFA"
BASE COUNT         8016 a         8797 c         8293 g         7820 t
ORIGIN      
        1 tttataatga ctgtgtattc ccatggaagg agatcaccca attctccccc cccgactttt
       61 gacgcagtgt cttgttaaca gggggattta ccatgcttat tggctatgta cgcgtatcaa
      121 caaatgacca gaataccgat ctacaacgta atgcgtttat ttgtgccgga agtgagttga
      181 ttgttgagcg aatgggaagt tggccagaag ttgttatggc atagtgttta cggtcgttga
      241 tacaggtaaa caaaagcggg gagagtcata ctccctgaat agtcgcgaaa atatctgtgg
      301 cattgtcaaa ttcgtgtcgg tgactaatat tttagtaata tgccattcga aatggagtgc
      361 taacttattg atttaattga tgtgcgatat tgtattttat cgtgttttgt tgtctgtttt
      421 ttatccagta agtgttgtta tgtgtatgaa agataaggga tttatcattt ttgaggtgtg
      481 ttcatggtaa gtaccaaacc acaacccgcc gcggcgactg aagacttcct gacgaactgt
      541 accgtccatt ttccacggat accctttggt atgctgttcg gcaaaagcaa tgctgacctg
      601 catcggccca ccggtgcgca ccggattaag cgaaccaaac agcgtctgcc ccattggtac
      661 catgctgatc agatcgtcaa aaatcgcgct caattgcttt tctgtacgca ccgaatctaa
      721 ccgttcgcta taacttttgc cgtttggcga tttgattttt agcgcggtat gaaccagaaa
      781 agcaggaatg tgcatccgtt cagcacgacg gtcaatctct tgccaggcga tcttgcttaa
      841 gcccggaacg gccggatctg cctgataatt tgactcctgc tgcgccaccg ccagcaccga
      901 gcagacgttt tccagcgtcg gtgccagccc ctgactttca aaggtgatgg cgagatcttt
      961 cgcccaggca tcgcggtctt tcacgctcgc aggcatcttc tgccgcacga cgctcgctac
     1021 atctatcgct ttttcgccct ttttcaacgg ttgcggagcc tgactactac accccgctaa
     1081 aaccagaacc gccaacaatg acagcgaaga tagatgattg acgcgcgaca ttacttctcc
     1141 tttttagctc aaggtatggg ttacttcctg aattttgtca ccatccagct catgttcaaa
     1201 gctgcgcaga cgtttgtaga tagacatcaa ctcaaccagt gtggtccatg agttaatcag
     1261 gtactggaaa gcaccgcgaa cctgaccaaa aacgttggta atctgcgtca tcaggccgag
     1321 cgtaatcgta ccggcaacaa ttgacggaaa cagcaagaac aaaccgaaaa cgttatcgac
     1381 ctgcaaatag agaatgcggg cgatgttgaa atacatatag tgaaaataga ggcgaaaata
     1441 gtttttccgt acggcgctaa acagctcgcg taccgtaggc ggcgtcgcgc gcgtggcatc
     1501 gtcttcacca taaaccagct ctttacggta ggcggcctct actcgctggt ttttaaactc
     1561 cagccccggc agtttgatcc ctaccactgc cagcaatccg gtccccatca gcgaccagac
     1621 gatagcggca atcaccagac catacggaat gtgcccgata atcggcagct ccggcacatg
     1681 cgcggagagc gttaccagca ccggcaggaa ggcgatcaac gtcatgatgg cgttgataaa
     1741 actgacgccc atattctcca gcgttgaagc aaaacgcatg gtgtcttcct gcacacgctg
     1801 tgcggcacct tcgatatgac gcagttgttg ccagttcgcc atgtaatatt cgttcatcgc
     1861 cgtacgccag cggaacacat agtgactgac aaagaagttg ttcagtacac tgatcaccac
     1921 cgcgatcagc gcaatcccca gaaagacgcc cacttcgcgg taaaattgtt cgatagtgac
     1981 tttatgcggc gaacttagcg cggtttgaat cagatcatag aacggtgcat accaggcgtt
     2041 gacggcgacc ccgacttcca ccaaaaacca ggtgacgaag atgatcagtg cagtaccgag
     2101 tattgaccag tattgccaac ggtgcgggct gtagataaac cagaaaaatg caaaaagtcc
     2161 tacgcaaaca atgtagtaag cgtaaaaaat caggaaatcc aacgaccaga aacgcgcggc
     2221 gctaatcggg atctgcccgg aagcgccggt gatacgcgcc acccagtctc ccccaccggc
     2281 ttgccagaag ataacggcaa tcaatgccca aacaaaggcc gagagaaaaa acgctcccgg
     2341 ctttgggaaa aaagacttaa acatatgcac tcctgctaac ttcttatcgt tttaacaact
     2401 gctgtgtatt aaccgcatga ccactacaaa gcgtcagcca aatgaaaccg cccattgtga
     2461 cccatagcaa ggcgcttagt tcgccagcct gaccataaga attgtttcga cggatttcac
     2521 aaaccaaaga ttgtttacat aagcggatta acacgcgcag gatcacaaat gcaggcattg
     2581 gtgagcagga aaaagatcgt gtttacgcgc cgttacgttt ttaacactat tattgcacta
     2641 attcgccctt tgcaatccat caatgagtag tataaatacg ctcagttacc ttcattcaat
     2701 ctatggacac caccgttgaa acgtagtctg cttttttctg ccgtgctgtg tgcggcgtca
     2761 ttgacctccg tccatgcggc gcagccgatc actgaaccgg agtttgcctc tgatattgtc
     2821 gatcgttatg ccgatcatat tttttacggc agcggtgcaa cggggatggc gctggttgtt
     2881 atcgacggta accagcgcgt ctttcgtagt tatggcgaaa cgcgacctgg taataacgtt
     2941 cgcccgcagc tggattcggt cgtgcgtatt gcttccctca ccaaactaat gaccagtgaa
     3001 atgctggtga aattgctcga ccaggggacc gtaaagttaa acgatccgct aagtaaatat
     3061 gctccgccag gcgcacgcgt gccgacctac aatgggacgc ccattaccct agtgaatctg
     3121 gcaacccata ccagcgccct gccccgtgaa cagcccggtg gcgcggcaca tcgtccggtg
     3181 tttgtctggc caacgcgcga gcaacgctgg aaataccttt ctacggcgaa gctgaaagcc
     3241 gcgccaggca gtcaggcggc gtactctaac cttgcgttcg acctgctggc agatgcactg
     3301 gcgaatgcgt ccggcaagcc ttatacccag ttgtttgaag agcagattac ccgtccattg
     3361 gggatgaaag acaccaccta catcccctca ccggatcagt gccgccgttt aatggttgct
     3421 gagcgcggtg ccagtccgtg caataacacg ctggcggcaa ttggcagcgg cggcgtctat
     3481 tccacgcctg gcgatatgat gcgctggatg cagcagtatt tgtcatctga tttttatcag
     3541 cgcagtaacc aggcagatcg catgcagacg ttgatttatc agcgcgcgca gtttacgaaa
     3601 gtgattggca tggatgtccc cggcaaagcg gatgcgcttg gtttaggctg ggtatatatg
     3661 gccccgaaag agggtcgtcc ggggattatt cagaagacag gcggtggtgg cggtttcatt
     3721 acctatatgg cgatgatccc gcagaagaat attggcgcgt ttgtggtggt cactcgttcg
     3781 ccgttgacgc gctttaaaaa tatgagcgat ggcatcaatg atctggtgac cgagctaagc
     3841 gggaataaac cgttggttat ccccgcatcc tgattaatac tctgaaggca aacggttgat
     3901 agcgaccagt ttgcctttat tcatttcgat atagccgcct ttacgcaaag cagcgagaac
     3961 ttctgcgacg acagaacgcg atatgcgtgt acgttgatgg atataattca tcacaccaat
     4021 acgtgatcgc aattcttcat tccagtcaat cattgataat aatgttgcgc ggatttgttc
     4081 gtaggaatta tgcccaatga gctgcacgtc gcgtaattcc agaattctat tttgccaggc
     4141 taaccagtaa aaagcgtctc gccagagttg attttgttca ataagcgtaa tggtttgttt
     4201 ggctggtaga tgatatcccg tacaatttcc ttctgatatt aatttgtatg gtatatcgtt
     4261 tttcattaaa ccatcagcca gccccataat ataaggggcc tgggtaatac cgataagtac
     4321 gttttcttct ctacgcagag agataactcc ctctaaaata acgaaagtat cttcgttatt
     4381 gacatcactg gaaaatataa cttgcttttc attattaaac tcgaagcgcg taccgtatct
     4441 ggacaaacat ttatcgagct taccaaattc ctgaagaggt ttaactacag ataacatttg
     4501 cgcgtccttt gcagtaatgc ccgtcaaatc cttgacgggc attatttaga ttaaattacc
     4561 agtatttctt cggagtgaag aatattacca ggtatattta acacccacgt tcgcggacca
     4621 gtcttgatct acgtcaccac caccgaggta gttagcatcg gtataggcgc tgaagttctt
     4681 ggtgaagctg aactgagtac ccagaccaac acgtaccgca gacccttcag taccgttatc
     4741 gatggaatca ccgttcacat cgttatcgtt gttagagtcg tcgtagacgt aagccagttt
     4801 gaagtacgga gtcagagcct gatcttcgct gtaggtgaag gtataacctg catctacacc
     4861 cagttcataa cgcatgctgt cgtaagactg accgtcaact ttcatgtcgt tgctcagctg
     4921 gtagtcatca ccagactgga acagaccaga aacgctgccg taaggagtca cgtaaccagc
     4981 atcacccagt ttgaagtcgt aaccggcttt caagccgaag ccccaagcgt cggagttggt
     5041 gctaccgtca acgtaagtac cgttgctcat ggttgcagac aggtcgttgt tgaagtgaga
     5101 gtagctcaag ctaccatcaa caaagacgtt gttcgcgaag tgagcagaag agtagatgta
     5161 ggcagtctgg ctgtcttgat ctacctgacc agaacggtca ttcatgtcac ctttagcgaa
     5221 gcctgcagcc gcaccgacga tccacttagc gttgttaccg tcaactttgg tatcaacacc
     5281 gaccatgatg ccgttaacat cctgatcata gttgatggtg ccgttgtcgc cgttgaagtt
     5341 accaccgaag tagcttaccc atgcgccgcc gttatcagcc aggccatgac gagagttggt
     5401 cagacgagta ccaacggtgt cttgttccag gttccagata ttggtgttcg cagacgggat
     5461 gctcagcgcc atgttagcgt agtcggtcag ctccatctgt tgcagaacaa cggtgttacc
     5521 gcgctgttca gcctgatagg tgtatgcacc caggtcagct ttgttagcag cagagaaggt
     5581 cgcgttggtt ttggtatcat cgatatagat gagctgctta cctgcgtaat cagcaatcgc
     5641 accagcgcca gtggagttgt cgatacgaac tttgtagtta ccggttgcag ctacaacgtt
     5701 gtctgcagag ctgttgtcaa cttcgtcgcc attgttaatg tcgttgttac cattaacggt
     5761 caagtgacca tcagagttca tcgcaataac accataaccg tagttagctt tgctggtgtc
     5821 gttggtacga tcgttgacga ggtctgcatt caggatgtaa tcattactat gaatgtcaaa
     5881 cacacctgcg taaacattac cgttgttatc tttaacgttg ccactggtca gttccagagt
     5941 atcagttgca aatacgctgg agatgtcatt catgttggtg tttacatcca gcacaccacc
     6001 gttagtaacg gtaatggtat tggcatacag cggtgcatat gcccaagctg aagcagtatc
     6061 ttcaccgaag ttaacggttg agaaggtatc aatggtcagg tgatcggtgt taacttcacc
     6121 gttaacttcc attgaagaac caccgttcag agtgatggtg tcagaagtca gagaagaatc
     6181 tgcactcacg ttaacctgag aaccgctgtt gatcgccagg gtatcgatca gggaagtttt
     6241 cgtggtttcc caatcagaac cgttgttcag aacaacatcg aatacgccgc tctggaatgc
     6301 gttagcgtta tcaacacgat tccagccatc ttcagagtaa gaagcgtacg ggttcaggct
     6361 gtttggtgca acgtcgtaga aggcttcgtc agcaacaaca acgtcataat caatattcgc
     6421 tgaaccaacc catttggtgt tgttcagagt caggttcagg tgatcgacgt tctgatcgtc
     6481 agttgcccaa ccgccatgag tgtaagcagt ggtagagttg tcaatcaggt gacccgtcgc
     6541 gtcaaagtta gcgttccagg tactgtcaaa tacaacgtca ccaagcaact gagagttgtt
     6601 gaacgtaacg ttgttgacca tacgataatc agaagtgtca tcatcgatga aggctaacgc
     6661 aacatcatca gcaggagatg cgccattgcc gtagtcactt ggttcaacag aattgccgaa
     6721 atgaccacga tctgattgtt cgtcttcagt ccatgaacca gaagttacag tagagtcatt
     6781 aacaacgatg ttgttgtccc actgatgacc ttcggtattg taggtcgcat taccgaggtc
     6841 gtgttgaacc aaagtaatac ctgcaacatg ggagttgttg ctgatgttga tgttactttc
     6901 aacatccaga gtcacagcgg taccaagacc agcggttacc aaacgatcat aatctgtctt
     6961 agatgtttta ccgtctgcat ccagataagt atcggtgaag tagaaggcgt caaaatcatc
     7021 gtcaatagta gaattagcga tgttcagagt gaagatgtcg ccatcaaccc agtcgtggcc
     7081 agtataatcg ctaccgttgg tccagacgta gtcgccataa ccaacctggt aagatgtaac
     7141 cataccatga acaattgagt tggagatgtt cagtgtatta ccggtatctg tgccataatc
     7201 gtggttcaga taataagtag aaattacacc atttacagtg tcgccgctga aagtggtatc
     7261 aaagtaacca tcagctgcgt ggtcatatac ataaccaccg taagtcagat aatcgtgatc
     7321 aattttagtg ctgtcacccc aattgtagat ggtggctgcg tcatcgtgat acccgaaggt
     7381 gttataagta gctgcattag cctgagaggt gatagccaga gtgcaagcca atgctaattg
     7441 tgatactaca agtttctttt tccaggagtg catttgtcat ccctcctcag ggacgtaata
     7501 agttgatcca tcaattatca atgcatagaa aacatattat gtttcgcgct aacgattatg
     7561 ctgcgttaca gacgaatggt tcaattattc tttcccctaa gtccgaatgt ggacatatat
     7621 aatttatgga gaaaaacacg catcaaattg ataaaaggac gatatttcaa catgttgcag
     7681 ataatgttat ttttgataca tacgcgttat aaatatcaaa aacacaaacc agataaatat
     7741 cttatcaata tacaacaaag tttgaattat tcaatttata gcagcacaac caaacgccaa
     7801 aaaaataata accaagagct ataaataaag attaaaaata ttttcagtga atatgaaatt
     7861 attgctattt ctattacaaa gatgtcctgg tctctctttc attctcttct tgtcaacatc
     7921 gcgacaactt tcgtaaaaca ctcctgcccc gcttcaggta tactgtgcac cttaattcca
     7981 caaacatcag gcagaccatg acagacttaa tccaacgccc tcgtcgccta cgcaaatctc
     8041 ctgcgctgcg cgctatgttt gaagagacaa cacttagcct taacgacctg gtgttgccga
     8101 tctttgttga agaagaaatt gacgactaca aagccgttga agccatgcca ggcgtgatgc
     8161 gcattccaga gaaacatctg gcacgcgaaa ttgaacgcat cgccaacgcc ggtatccgtt
     8221 ccgtaatgac ttttggcatc tctcaccata ccgatgaaac cggcagcgac gcctggcggg
     8281 aagatggact ggtggcgcgt atgtcgcgca tctgcaagca gactgtgcca gaaatgatcg
     8341 tcatgtcaga cacttgtttc tgtgaataca cttctcacgg tcactgcggt gtgctgtgcg
     8401 agcatggcgt cgacaacgac gcgactctgg aaaatttagg caagcaagcc gtggttgcag
     8461 ctgctgcagg cgcagacttc atcgcccctt ccgccgcgat ggacggccag gtacaggcga
     8521 ttcgtcaggc gctggacgct gcaggcttta aagatacggc gattatgtcg tattcgacca
     8581 agttcgcctc ttccttttat ggcccgttcc gtgaagctgc cggaagcgca ttaaaaggcg
     8641 accgcaaaag ctatcagatg aacccaatga accgtcgcga ggcgattcgt gaatcactgc
     8701 tggatgaagc ccagggcgca gactgcctga tggttaaacc tgctggagcg tacctcgaca
     8761 tcgtgcgtga gctgcgtgaa cgtactgaat tgccgattgg cgcgtatcag gtgagcggtg
     8821 agtacgcgat gattaagttc gccgcgctgg cgggtgctat agatgaagag aaagtcgtgc
     8881 tcgaaagctt aggttcaatt aagcgtgcgg gtgcggatct gattttcagc tactttgcgc
     8941 tggatttggc tgagaagaag attctgcgtt aatttttttc ctctcagaaa gggggagtta
     9001 acctgttgaa agtacggtga acgccccctc accctaaccc tctccccaag ggggcgaggg
     9061 aaccgtccgc actcgtatca ccccgcccga taaaacggtt tatccccaag gatcgtcgcc
     9121 cgatgcataa tccgccgctg tggcaggtaa tcggcattgg catagtgctg agtcacgcgg
     9181 ttatcccaaa tcgcaatatc attcggttgc cagcgccaac gcacctgaaa ctccggttta
     9241 gtgatatggg cgaataaaaa gcctaacaag gcttcgctct ctttctcgct gacatcgaca
     9301 attcgcgtag taaagccttc attcacaaac agcgcctgtt taccgctcac cggatgcgtt
     9361 cgcaccaccg gatgtagcaa cggaggattt ttcgcgaccg cctcgcgcca gcgttgatgt
     9421 tcctcctcgg ttttgcggta tttgtattcc gggaacgatt tacggaaatc atgctccgca
     9481 cgcagcccgc tcagcagctg gcggaaggga acagagagcg cctcataggc cgcaataccg
     9541 ctggtccaga gcgtatcacc gccggtcgaa ggtaactctt tagctgccag aatcgcccct
     9601 gcgggtggcg tttcaataaa tgtcacatcg gtatgccagt tgtcgttatc cggcggatta
     9661 tcgttatggg tatccagcac gatgatctcg tcaacccctt cggcatgagg gtaaacaggg
     9721 tgaatatgca gttcgccaaa acgctgggcc agcgcgcgtt gctgctgcgg cgtaatagct
     9781 tgatcgcgca gaaataccac ctgatggcgc agcaccgcat ggtaaagctg ttcaaactga
     9841 ttatcgctta acgggcgcgt caggtcggca cccgaaattt gtgcgccgat atacggcccc
     9901 agcggggtaa tactcagacg ttcactcatt gtacttctcc atgccagggc gtcagacggc
     9961 gctgtaacgc gcgcagaccc agttctaaaa gaaaggcgat aatcgcaatc accgcgatcc
    10021 ccgccagtac cacgtcagtc gcgagaaatt cacccgctga ctgaaccata aatcctaaac
    10081 cgcgtgtcgc ggcaatcagc tccgccgcta ccagcgtaga ccagcccaca cccagaccaa
    10141 tgcgtaatcc ggtgaggatt tccggcagcg cgccgggcaa aatgacaaac cacagcacct
    10201 gcgcacggct ggcacccagc gactgggcgg cacgaatgcg aacctgctgc gcgcttttca
    10261 cccccgccag caccgacatc gccaccggtg caaaaatcgc taaatagatc aataagatct
    10321 tcgaggtttc accaatacca aaccagatca ccatcagcgg caaataagcc agcggcggca
    10381 ccggacgata aagttcgatt accggatcca gaatgccgcg caccgtcggg cttagcccca
    10441 tggcgatccc gaccggaata ccgatcacca ccgccgcgag tagcgccagc acaatgcgcg
    10501 tcaggctggc tgccagatgc tgccacagcg tggcgtccat aaatccctgc ggtccggcaa
    10561 tggtgagcag ttttgccagt acttgttgcg gcggcggcaa aaacagcggg ctaatcagtt
    10621 gcagcgccgc caccgtccac catacagtga gtaaaaccgc taacgtgcca atgcttaagg
    10681 tcacctgacg cgagagcggc cagcgccatt tcagccgccg cgaatgcagt ttttcattaa
    10741 tgagcacact catgagaacg cctcccgttg ctcaaatacg cggcttaaaa catattcgcg
    10801 catggcgatg aattgtggat cggacttgat gctgcggctc gactctcccg caacaaagcg
    10861 gcgagcaaag ttgagcggca gccgctccag cacacggcca gggccggatg aaagcagaac
    10921 cagttcagtc gccataaaca ccgcttcttc tatatcgtgg gtaatcaaca gcacctgctt
    10981 gcccgtctcc tgccagagtt tcagcagcag agtttgcatc tggtcgcggg tgaaggcgtc
    11041 cagcgcacca aacggttcgt cgagtaataa cagctgggga ttcgccgcca gcgcgcgggc
    11101 aatccccacc cgctgacgtt gaccaccgga aagctgccag atatagcgtt tttctgcgcc
    11161 ttccagcccc acttttttca gcatctggtg cgcgatttcc agtcgctgca ttttctctat
    11221 acctgccaat tgcaggccga acgccacgtt gtcctgtaca ttgcgccacg gtagtagccc
    11281 ttcattctga aaaaccaccc cgcgctctgc tcccggtccc tcaatacgct tacccgccag
    11341 ttgaatgctg ccatgctgat aaggcacaaa accggcaatc agattcagca gggtggtttt
    11401 accgcagccg gacggcccca gcaccaccag tagctcgccg ctttccagcg tcaggttgat
    11461 atcttccagt gccggtttgc cgccataatc ggcatagaga tgagagattt gcagcatctg
    11521 cgcctccttt tattgcacga agcgcgaggt aacgtactgg ctgtaatcat tcgctacagc
    11581 cgggaccttg ccctgctctt tcaaaaactg cgcggtgtcg atgattgctt tgttcaccgg
    11641 tccggtcagt tctgccgttt gttgctgcgg cgtcagatag gtattcccct tcaccagccc
    11701 cggaacgtca ccttccggca cgccgcttaa acgcgccagt ttgctgatgt tttccggctg
    11761 tttcagccac gcgtctgggt tagcaatgta cggttgctga gcatcgatgg cgcttttagc
    11821 gaacgctttc acgacctcag gatgtttctc ggcaaaatct ttgcgcacca cccagacgtc
    11881 cagcgttggc gcaccccact gcccgacctg ttcagaatcg gtcaacacct tgccgtcttt
    11941 ttccagggcg ttaaccgccg gtgcccagac ataagcacca tcaatatcac cccgctgcca
    12001 tgcggcgata atcgcgggcg gctgcaggtt cacaatctcc acttgccctg gtttaatccc
    12061 ccagtgtttc agcgccgcca gcaggctgta gtgggtggtg gagataaacg gtacggcaat
    12121 gcgtttgcca atcaaatctt cgggtttgct gatagttttc tttaccacca gcgcttcgga
    12181 gttacccagt tttgacgcca gcaagaagac ttcaatcggc acctgttggc tggctgcaac
    12241 cgctaatggg ctggaaccga ggttgccgat ttgcacgtcg ccagaagcca gcgcccgcac
    12301 gatgctggct ccgctgtcaa acttacgcca gtccacgctc gccccgctct ctttagcaaa
    12361 ggtgttgtcg gcctgagcca ctttcgccgg ttcggctgag gtttgatacg ccacggtgac
    12421 gttcaccgcc tgtgcctgaa aagcgatgaa tgccagtgcg gcaagaagtg tgttacgcga
    12481 tgaaattgcc atgattgtct gctcccctgt cttgttatgg gagcagtatt caggaataaa
    12541 aacattcatt aaaagaatta gtcgttatcg cacagatgat tttattctta gcaaaaaaac
    12601 ggcaaaggaa agattaaata tgcaattact ccgttctaac aattgggaaa attactacac
    12661 ttcattttag tgatgcataa acaaccacat tacgagattt attacttcat tgtttctatc
    12721 aatacatata ataaaactat tatattcgca ttgtatatat cactgtatca ttctcaatca
    12781 ctcgcatatt acattcacat aatgtatata aacaatactt gttcacagaa catatttttt
    12841 aaatgcaatt aattgacaaa ctaaatcaca tctaaataca atgtataaaa caaaataact
    12901 atacatacca aacatacaaa cacttaacat atgaattgca taaaaacatt acccagtcga
    12961 acctccgggt ctgaaagccc ttgcttttca gttattagca acatttaaca tttataaatt
    13021 aacatctggc tgcgccagag attacgtctc tgagtgcatc gttgtgcttg tagtataata
    13081 aataaaatgg agtgtcatta agtggataag gttttagatt cagccctcct ttcttcggca
    13141 aataaaagaa agggtatcct tgctattggc gcacatcctg acgatataga attaggctgt
    13201 ggcgcatcgc ttgctcgtct tgcgcaaaaa ggaatttata tcgcagcagt ggtgatgact
    13261 accggcaact ctggtacaga tggaataatc gatcgccatg aagaatcgcg caacgcccta
    13321 aagatattag ggtgccacca aactattcat cttaattttg ctgacacccg cgctcattta
    13381 cagctcaatg atatgatttc cgccctggaa gacatcatta aaaatcaaat tccttctgat
    13441 gttgaaatca tgcgggtata taccatgcat gatgccgacc gccatcagga tcatctcgct
    13501 gtttatcaag cttcaatggt tgcctgccgc actattccac aaattctcgg ctacgaaacc
    13561 ccaagtacct ggctttcatt tatgcctcag gtttttgaat ccgttaaaga agaatatttc
    13621 acagtcaaac ttgctgcatt aaaaaaacat aaaagccagg aacgacgtga ttatatgcgt
    13681 catgatcgcc tgcgtgcagt tgcacaattt cgcgggcaac aggtcaatag cgatctgggt
    13741 gaaggctttg taattcataa aatgattctt tgagcaaatt gcatgaaaac ctggatattt
    13801 atctgtatgt ccatagcaat gttgctatgg tttttaagta cgctaagacg taaacccagt
    13861 caaaagaaag gctgtattga cgccattata cctgcgtata acgaaggccc gtgtctggcg
    13921 cagtcactgg ataatctact gcgtaaccct tatttttgcc gggtaatttg cgttaacgac
    13981 ggctccacgg acaataccga agcggtcatg gcggaagtca aacgcaaatg gggcgaccgc
    14041 tttgttgccg tcacgcaaaa aaataccggt aaaggtggtg cgctgatgaa tggcctcaat
    14101 tatgccacct gcgaccaggt ttttttaagt gatgccgaca cctatgttcc gcccgatcaa
    14161 gacggaatgg gctatatgct ggcagaaatt aagcgcggtg ctgatgccgt aggcggcatt
    14221 ccctctactg cgttgaaagg cgcggggctg ttaccgcaca tccgcgcgac cgtaaagttg
    14281 ccgatgattg ttatgaagcg cacgctacag cagctcctgg gcggcgcacc gtttattatc
    14341 agcggtgcct gcggaatgtt ccgtactgat gtattgcgta agttcggttt ctcggatcgt
    14401 actaaagtcg aagaccttga tctcacctgg acattagtgg caaacggcta ccgtattcgg
    14461 caggcgaatc gctgcatcgt atacccacag gaatgcaaca gtccgcgtga ggagtggcgt
    14521 cgctggcggc gttggattgt gggctacgcg gtctgtatgc gcctgcataa aaggctttta
    14581 tttagccgct tcggtatctt cagtatattt cctatgctgt tggttgtgat ttatggcgtt
    14641 gggatttatc tcactacctg gtttaatgaa ttcatcacca ccgggccgca tggtgtggtg
    14701 ttggcaatgt ttccgcttat ctggatcggc gtagtttgtg ttattggtgc ttttagcgcc
    14761 tggtttcatc gttgctggtt gttggtgcct ttagcgccgc tttccgttgt gtatgtatta
    14821 ttagcttatg ccatctggat tatctatgga cttattgcct tttttactgg acgcgaacct
    14881 cagcgcgaca aacccacccg ctattctgca ctggtggaag cgtcaaccgc ttattcccaa
    14941 ccttctgtca caggaactga aaaactatct gaagcttaat gttaaagaga aaaattattc
    15001 agattgcaga ccaggtaatt attgatgaaa ctgcaggtga agttgttatc ggcgcgaata
    15061 cccgtatttg tcatggtgcc gttattcagg gtccggtagt gattggcgca aactgcctga
    15121 taggtaatta tgcgtttatt cgtcctggca caataatcag caatggtgta aaaattggtt
    15181 ttgccaccga aattaaaaat gcggttattg aagcggaagc aacgattggt ccgcaatgtt
    15241 atattgccga ctcggtagtt gcaaaccagg catatttggg cgcacaagta cgtaccagta
    15301 atcatcgtct ggatgaacag cctgtgtctg ttcgaactcc agagggaatt atcgctaccg
    15361 gatgcgataa attaggttgt tatatcgggc agcgttcacg ccttggcgta caagttatta
    15421 ttttgcctgg gcgaattatt tccccgaaca cgcaacttgg cccgcgcgtg attgtagaac
    15481 gtaatttacc taccggaact tattcactcc gacaagaact tatccgtaca ggagattaaa
    15541 atgataaaac ggacgttatt agcggcggcc atttttagcg cattgcccgc ttatgccggg
    15601 ttaacttcca ttaccgcggg ctacgatttt accgattatt ctggcgatca tggcaaccgt
    15661 aatttagcgt atgctgaact ggtggcgaaa gttgaaaacg caacgctgct ttttaatctt
    15721 tcccaggggc gtcgtgatta tgaaactgaa catttcaatg ccactcgcgg tcagggtgcg
    15781 gtctggtata aatggaataa ctggctgaca acccgaacgg gtattgcctt tgcggataat
    15841 acgccggtct ttgcccgcca ggattttcgt caggatatta acctggccct gttgccaaaa
    15901 acgcttttca cgaccggtta tcgctacact aaatattacg atgatgtcga agtcgatgcc
    15961 tggcaaggcg gcgtatcact ctatactggc ccggtgatca ccagctaccg ctatacccat
    16021 tatgactcca gcgatgcagg tggtagttat agcaatatga tttccgtgcg tctgaatgac
    16081 ccgcgcggca ctggttatac gcaactatgg ctaagccgcg gaacaggcgc ttacacctat
    16141 gactggacgc cagaaacacg ctacggcagc atgaagagcg tcagtttgca acgtattcaa
    16201 ccgctgactg agcaacttaa tctcgggctg acggcaggta aagtgtggta cgacacccca
    16261 accgatgatt ataacggtct gcaacttgca gcccatctga cctggaaatt ctgattgctt
    16321 ctgccgcccg ctatccgggg cggccttccc tgccgattag ccccactccc tttcctcgtt
    16381 gttttccgac cacattcacc ggataaattt tattctccag cgttatatac tatagggagg
    16441 tatgcattga catatagcat acccccctat agtatattgc gtgcagatga tgaggtgcga
    16501 aatgcccagt actccggaag agaagaaaaa ggtccttact cgagttcgtc gtattcgggg
    16561 gcagattgat gctctggaac ggtcgctgga gggtgatgcc gaatgccgtg ccatactcca
    16621 acagatcgct gccgttcggg gcgcggctaa cgggctgatg gcagaagtgc ttgaaagcca
    16681 tatccgggaa acgtttgacc gaaatgactg ctacagccgc gaagtcagcc aatccgttga
    16741 cgacactatt gaactggttc gagcctatct taaatagctg aatctattac catattgagg
    16801 aagagcgaga gatgaaatca cgtgctgccg ttgcatttgc tcccggtaaa ccgctggaaa
    16861 tcgttgaaat tgacgttgca ccaccgaaaa aaggtgaagt gctgattaaa gtcacccata
    16921 ccggcgtttg ccataccgac gcatttaccc tctccgggga tgacccggaa ggtgtattcc
    16981 cggtggttct cggtcacgaa ggggccggcg ttgtggttga agtcggtgaa ggcgtaacca
    17041 gcgtcaaacc tggcgaccat gtgatcccgc tttacaccgc ggagtgcggc gagtgtgagt
    17101 tctgtcgttc tggcaaaact aacctctgtg ttgcggttcg cgaaacccag ggtaaaggct
    17161 tgatgccaga cggcaccacc cgtttttctt acaacgggca gccgctttat cactacatgg
    17221 ggtgctctac attcagtgaa tacaccgtgg tcgcggaagt gtctctggcc aaaattaatc
    17281 cagaagcaaa ccatgaacac gtctgcctgc tgggctgtgg cgtgaccacc ggtattggcg
    17341 cggtgcacaa cacagctaaa gtccagccag gtgattctgt tgccgtgttt ggtcttggcg
    17401 cgattggtct ggcagtggtt cagggcgcgc gtcaggcgaa agcgggacgg attatcgcta
    17461 tcgataccaa cccgaagaaa ttcgatctgg cgcgtcgctt cggtgctacc gactgcatta
    17521 acccgaatga ctacgacaaa ccgattaaag atgtcctgct ggatatcaac aaatggggta
    17581 tcgaccatac ctttgaatgc atcggtaacg tcaacgtgat gcgtgcggcg ctggaaagtg
    17641 cgcaccgcgg ctggggtcag tcggtgatca tcggggtggc aggttctggt caggaaatct
    17701 ccacccgtcc attccagttg gtcactggtc gcgtatggaa aggttccgcg tttggcggcg
    17761 tgaaaggtcg ttcccagtta ccgggtatgg ttgaagatgc gatgaaaggt gatatcgatc
    17821 tggaaccgtt tgtcacgcat accatgagcc ttgatgaaat taatgacgcc ttcgacctga
    17881 tgcatgaagg caaatccatt cgaaccgtaa ttcgttactg atttcccgca ggtatacccc
    17941 gtccacttca gacggggttc ttaatactct ccccgggcag ccgtccgggg gattaaccgt
    18001 gagataatga ctgatggaac tcattgaaaa acatgccagc tttggcggct ggcaaaatgt
    18061 gtatcggcat tattcccaat cactgaaatg tgaaatgaat gtcggcgtct atctcccacc
    18121 aaaagccgcg aatgaaaaat tgccggtgct gtactggctt tcaggcctga cctgcaacga
    18181 gcagaatttc attactaaat cggggatgca gcgttacgcg gctgagcaca acattattgt
    18241 tgttgcgccg gacaccagtc cgcgaggcag tcatgtcgca gatgccgacc gttacgatct
    18301 cgggcaaggt gccgggtttt acctgaacgc aacgcaagcg ccgtggaatg aacattacaa
    18361 aatgtatgac tatatccgca acgagctgcc ggatttagtg atgcaacatt ttccggcaac
    18421 gaccagaaag tctatctctg gtcattctat gggcgggctg ggcgcgctgg tgctggcgtt
    18481 acgtaaccca gatgaatatg tcagcgtctc ggcgttttcg cccattgtct ccccatcgca
    18541 agtaccgtgg ggacagcaag cctttgccgc atatcttggt gaaaataaag atgcctggtt
    18601 ggattacgat ccggtgagtc ttatttcaca aggtcaacgc gttgcggaaa tcatggttga
    18661 tcaggggttg agtgatgatt tttacgcgga acagctgcgg actccaaatc ttgaaaagat
    18721 ctgccaggag atgaatatca agacgttaat ccgttatcac gagggttacg accacagcta
    18781 ttattttgtc tccagtttta ttggcgagca tattgcctac cacgccaata aactgaatat
    18841 gcgttgataa tagcacgact gccggatgcg gcgtgaacgc cttatccggc ctacacttcg
    18901 cccgtaaacc gtaggcctga taagacgcgc aaagcgtcgc atcaggcatg gcgcacgact
    18961 gccggatgcg gcgtgaacgc cttatccggt ctacacttcg cccataaacc gtaggcctga
    19021 taagacgcgc atagcgtcgc atcaggcatc tgcgcacgac ttatctatgt ttaccacatc
    19081 aaatcatcag gcactttgaa gtcggcatac ggatcgtctt catcctgctc ttcggcactg
    19141 agcgcgctgt gcaacacaat actgctggca tcacgctgag caattttatc ggcaacactg
    19201 gcgggaataa tggtgtattc accttcgcta ttgttatcga ccaacaagcg ggcaatcgcc
    19261 agacgaccat taatcaactg cgcctgtgtg agcttatcga caaaaatctt cttaatcaga
    19321 ttgccgtcag tgaagttaaa accaatatcg ccattggcaa tggtgattcg gttcatttca
    19381 atgagctgct tcacctgggc tttatattct ttcgccaacg ccgcttgttt ttgctgttcg
    19441 ctaagctgtt tatcacgctc aagctgtgcc tttttatttt cttctaccgc agcccgagct
    19501 tcacgcgcct gaacgcgcga ttttttcgcc gttctctcga ccttcgccgc ttttttgctg
    19561 gtgactaatc ctgctttgag caactgctct tgtaaggtaa gttttgccat ctttgtttcc
    19621 aaacccgctg aataattttt agcgagtata cctgtaaacg ccgtgggtgt attgggggca
    19681 agtccctttc gcccctgctt aataaggcgc gtcaggcatc ggcacacggc tgcattcttg
    19741 atctccgact catcaaaata aacaccgcca acccagcaac aagaataccc ggtgcagaag
    19801 cggccattac gccgaccgtg ccagtgccta atgccagcat tttcccggcc agtaacggac
    19861 cgctcatagc ccccagacgc cctaccgcca cggctgttcc cacacctgtt gcgcggatct
    19921 gcgaactgta aaacaacggt gccagggcat acaaaacgct ttgcccacct gtcgcaaaca
    19981 accccgcgac aaatcccgcc agcaacatac cgttaaatga cgacaccgtt ccaagcgcca
    20041 gcagcgaagc taacatgccg ctataaatca gtagcgacat ggttactgga cgcagcttat
    20101 ccatcaatgc gcccaacatt aacgtcccgc ttgccgcccc catttgcagg gcaaacatca
    20161 cccctgccgc ctgcgatggc tggaatcctt gctccaccaa aagtagcggt agccagttga
    20221 tcaacatata aaccaccagc agagtgaaga aataacacaa ccacagcagc agcgtcgcgg
    20281 ttgccgtttc tggcgcaaat aaggcacgca gtggtggcgc agcctgtttt tcgccagcga
    20341 aaaccgctga ctccggcagc cagcgcatta atagcggcac cagaatcaac ggcaccacac
    20401 cacctaccca aaacaccgtt tgccatgcta agtttgcccc cgcgaaaccc agtgtcgccg
    20461 ccagcgccgc gccaatggga acaccgcaat acatcaggct cactgccgtc ccacgaaaac
    20521 gtggacccgc ggcttcagat gtcagggcga taagattcgg caacgccgcc cccagcccga
    20581 caccggtcat cagccgcgca aagaccagtg aggggaaatc ccaggcaatc gccgttgcca
    20641 gtgagaacaa accaaacagc gcaactgagc caatcaaaat acgcttacga ccataacggt
    20701 ctgccagcat cccgccaacc aacgcgccgg gtagcaaacc gagtattccg gcgctaaata
    20761 tccagcccat ttgcatttta tcgagtgcga aagcctgggc gatgccaccc gccgcaatgc
    20821 cagccgcctg aagatccagc ccttccatca gagcgaccaa aaaacaaagc ccgatggtca
    20881 gcatcaggcg ggatgaagat gatgaagggg tacgagtcga cataagatga acctcattgc
    20941 gagtgcaggt gaatcgcgac tgatacattg ccagccgcgt aatcagaact atttaatgac
    21001 tgcctacggg cgaaatgtag cgaaatgtag gccggataag gcgttcacgc cgcatccggc
    21061 tgtcgtgctg ggatgggcat acaaaatgca cgttatcggc agtcatcatt atttgttgtt
    21121 gcgcagatcc agcgccacgt cgacgatcat atcctcctgg ccgccaacca tccggcgttt
    21181 gcccagctca acgagaatat ccaccgcact taagccataa cgcgccgccg ccgtttcaca
    21241 gtgacgcagg aagctcgagt aaacaccagc gtatcccagc gccagcgttt cgcgatcgac
    21301 tcgtaccgga cgatcctgca acggacgcac caggtcgtcg gcggcatcca ttaacgcata
    21361 gagatcggtc ccatgctgcc agcccagttt atccgcggcg gcaataaaca cttccagcgg
    21421 tgcgttacct gcgcccgcgc ccattcccgc gaggctggcg tcgattcggt cgcagccctc
    21481 ttccaccgcc gcgatagagt tcgccacgcc aagactcagg ttatggtgag cgtgcatgcc
    21541 ggtttgcgtt tctggtttca acactgcttt cagggcgcgg aaacggtcac ggatatcgct
    21601 catgttcatc gcaccgccag aatccaccac ataaatacag gtcgcaccgt agccttccat
    21661 cagctttgcc tgcttggcga gattctccgg cgtggtcata tggctcatca tcagaaaacc
    21721 aacggtgtcc attccgagct cgcgggcata ctgaatatgc tgggcggaaa catcagcttc
    21781 ggtacagtgc gttgccacac gaaccacccg cgcgccagcc tgccaggcat ttttcagatc
    21841 gtgaatagtg ccgattcctg gcagcaacaa cgtcgcgatt ttggcgtgcc tcaccacatc
    21901 cgccgccgct tcaatccatt caaggtcgct atgtgcgccg aaaccatagt taaagctgga
    21961 accttgcaaa ccgtcgccgt gggccacttc aatcgaatcc acgcgggcat cgtccagtgc
    22021 tttggcaatc tggcgaacgt tttccagcga atactgatga cgaatggcgt gcataccgtc
    22081 acgcaatgtg acgtccgaga tataaagttt tttaccgttc atgccgcttc tcctgccttg
    22141 cgcgccagtg actgggccat tttttccgct gtcgccagcg cactggaagt cataatatcg
    22201 aggttgcccg cataggcagg cagataatgc gctgcgcctt cgacttccag ccagaccgct
    22261 gttttcagtc cggagaattg ccccacgccc ggtaaattga ccggtttatc ctgcgggata
    22321 acttcaaact gcacgcgctg tttcaggcga taacccggta cgtaagcctg caccgcctca
    22381 gccatttcat tgattgaggc ttcgatatca tcttgtgaag cttcgtcgct caatacatac
    22441 accgtgtcac gcatcatcaa cggtggctct gctgggttaa gcacaataat cgccttccct
    22501 tttgccgcgc cgcccaccac ttcaatggct cgggaagtgg tttccgtaaa ttcatcgata
    22561 ttggcacgcg tgccaggtcc ggcagattta ctggcgatag aagcgataat ttcggcgtaa
    22621 tgaacacgcg ccacgcgtga aactgccgcc accattggaa tggtggcctg gccgccgcag
    22681 gtgaccatgt tgacgttcag ttgatcgacg ttcgcctcga ggttaaccac cggcacgcag
    22741 taagggccga tggcagcagg cgtcaggtca attaagcgaa tatccggttt cgcttcgcgt
    22801 aaagcggcat cgtttttcac atgcgcgccc gcgctggtcg catcaaatac aatgtcgata
    22861 tcagcaaatt caggcatgtt catcagcccg atcacccctt catgggtggt ggcgacgccc
    22921 atacgtctgg cacgcgccag accgtcggac tgaggatcaa tgccgaccat caccgccatc
    22981 tccagatgct gaccgtgacg caaaatttta atcatcagat cggtgccaat gttgccagaa
    23041 ccgataatgg cgactttacg cttactcatg acagacttcc ttttggggct gcgcttgaaa
    23101 atgtcgcagc aactgaacct atgccttcaa tatgggcttc aaaacgatcg cccgcattca
    23161 ccgccaccat cgggcctaat gccccggtaa gaatgatatc tccggcgcgc agcggttcac
    23221 ccagactggc cattttgcgt gccagccaga cggcggcatt aagcggatgt cccaggcatt
    23281 cgctgccgcg cccgctggaa acctcttcgt tattacgcgt catcttcatg gcgcagtttt
    23341 tcaggtctaa ccccgccgga cgttgcgccg gaccgccgat gacatacacc ccgcaggaag
    23401 cgttatctgc cacggtatcg acaaactgaa tcgaccagtc gcgaatgcgg ctccccacca
    23461 cttccagcgc cggaagtacc cattcaatgg cgttatacag ttcgtcgaag gtgatatcag
    23521 ttgcgggtaa atcgcggttc aacaccagtg cgatctccgc ttcaatgcgg ggttgcagaa
    23581 cacgggaaaa aggaatgatt tcgttatcgc cataacacat gtcggcaaat aacgtcccaa
    23641 aatccggttg atcaacgccc agttgttgtt gcactttcgg atgcgtcagg cccactttac
    23701 gccctaccac gcgacgcccc tgcacaacgt catattgcac atttatgtgc tgaatggcgt
    23761 aggccgcttc agcgtgatcg ataccaatca gatcgcgcag cggcgcaatc gcttcgccct
    23821 gctctgcggc gcggcgtaaa tccgccgcca gttgctcaag agtatgcttc gtcatgacca
    23881 ttccttaagg gcgtgcgagg aaattcagca ccaattgatt gaaagcgtcg gcatgttccc
    23941 actgcgccca gtgaccacag tcgcggaaga tatgcagttc agaaccggca atgccggaca
    24001 gcagacgcag acccgcatcc atcggcacaa agcggtcgtt gcgcccccag acaatcaggg
    24061 tttgcgcttt gatttccgcc agacgtgggc caaaatccgg gaactgtttc ggattagctt
    24121 ccaggctctt aacgaagttt tccaggtgat cgcggcgcga cagcatatta ttcaggcgcg
    24181 cttcaaacag ggcgtcggtc aaatcgctgg tatcgaaaac gaagatatcc atcatcagct
    24241 tcaggttttc gatagtcggc tgacgataaa gctgattcag tcgcttaata ccttcggttg
    24301 gcatcggcgt aaacaaactc atgccgcccg taccgccgcc catcagcacc agtttgccga
    24361 cgcgctccgg ccagttaagg gtgaacgcca cagaactatg gccgcccatc gagttgccca
    24421 gcaggtggat tttggcgata tccagttgat ccaccacgct tttcaggatt cgtgcattaa
    24481 gatccgatcg tgaaccacta ttaacgatcg aatcgctttt gccccaaccc ggacaatcca
    24541 gcaggatcac ccgatagccc gcctctacca gcggatcgat attgcggctg aagttcgccc
    24601 agccagtagc acccgggccg gaaccatgca gcaggacaac ggtttcgtcg ccttgtccgc
    24661 agtcattaaa atggatgcgc agcgttttac ccgcttcttc tacattcaga aaacggctgg
    24721 tggcggcttc ggtttgtggc tgataactca tcatcttctc ctgcatattc agttctctgt
    24781 tctggcgctt aacgagccaa atccggcaat ccactccggg attgggcggt aataacgccc
    24841 ttcgctacgc cagttgccaa atgcagaaat agcggcaaaa gcggcgaccc aggttttgat
    24901 ttcatgtgtc gactttccgg caatggcgga aagctcttcg ttactgacgg catccagttc
    24961 ctgtatgcgt ccctgctcca gcaaagtcat gaactggtta tcccaaatcg ggttgagcgg
    25021 atgcagagtt ctctgatcct caacaaactt ctcagcggcg ctaatcaccc tttgctgacg
    25081 caattcgcgc tcactggcgg gtaaatcttt cccgctcccc aacagacggt cgcgcatatg
    25141 ggcatcggct ttcgccagtt cgggcaccgg cggctgatgg gaaagcccac cggaacccag
    25201 gaacagcacg cgtttattga gagtgctggt gaaacgtcca atggcttcac ccaacatgcg
    25261 ggtacgctgg aaaccgggca gcggcgtggc gacaccgttg atgaacacag gcagaactgg
    25321 caccttatcc agcccaccga gcaggaactc cagcggctgg gcgaacccgt ggtccacctg
    25381 catacagtaa gaaacggcaa gatcgatccc gctcttcatg acagcatgcg cacaggcctc
    25441 cgccagctcc acaggcacgg gcagctctcc tgccgcactg ccgaaatcac caattgccgt
    25501 cgctccaacg cctaaacaga acggtggcat cacgtcatag aaaaagccgt tgtagtgatc
    25561 tggcgcaaac agcaccacca gttcagggga gaaagccgca atacgctcgc gggcgctggc
    25621 aatcacgcca ttgacctcat cgagtacctc ttgcgccggg tcgacatacc ccaccaacgg
    25681 cgagtgggaa agacagtgaa gataagcgtg catatcaggc taccttttcg acagaaacgt
    25741 cggcatcagg gcgggtcagc gtcatcaccg acgccagttt attcagcgta ttgcccaggg
    25801 tttgcggaat ggcggtggcg gcaacaaagc gatccgggcg catcaccacc agcgaagcat
    25861 tgtgttgcgc gaaccagcta cgcagacgac cttgcgtatc gcccacgcgt agcacgccgt
    25921 cgtggttatc ctgtgcggta tgaatttgca cttccggcac cacctgaatg aagcgtgtgc
    25981 ccaacgcgcg ccactgctgg atttgctcgt cgctcatccc ccacagtgga ttgcatcccc
    26041 agccaattac cgcgaagttc gcgccgatcg cgttatcgag cagcgtcacg tcgccgtttt
    26101 ccagcgtgac tttcggctga ataaacatct tgccgaccgg agagtgcttc gcttcgccct
    26161 cacgcaccag cgcaccgccg taatattgcg gcatcggctt gaagcgcatt tcgaggaagt
    26221 agcgttttac tggcggcaga taattcaaca gccaggaaac gccgtcacgt aacgtaccct
    26281 gccagcgttt cggcggagcc agcacgttgc ccgccgtcac ggacaggtca atcatcgctt
    26341 tggcgtgatc gcgacgttct tgttgatagg tatcgagcag cgcatcgcgg gctttcccct
    26401 ggataaccaa cgccagtttc caggcgaggt taaaggcgtc gcgcatacca ctgttatagc
    26461 cctgcccctg ccacaccggc atgatgtgcg cggcatcgcc cgccagcagt acgcgatcaa
    26521 tacggaaacg ttgcgccagt cgcgcgttat gggtgtagac acgctggcga atcaattcaa
    26581 cattgtccgg attaggcagc actttgctta acagcttgcg catattttgc ggctcacgca
    26641 gctgctcttc ggtttctccc ggcatcacca taaattcaaa gcgacgtacc gcatgaggca
    26701 gcgcggcaga aacatacggg cgcaccggat cgcaacacaa atagatatgc ggcgtactta
    26761 acggatcgtt ggcgatatct accacaatcc actgatttgg cgcagtttta ccttcaaacg
    26821 gcacattcag ggtgcgacga acaaaacttg ccccaccatc acaggccacc agccactggg
    26881 ctttgatcgt ttcccgctgc ccttctgccg ttttcaggtg caaggtcact tcgtcatctt
    26941 gctggctgaa ggcctccagc tcgcgggaaa acaagcagcg cacattcgga aaacgcgaca
    27001 acccttccag catcaccgca tcgacctgtg gctgaataaa ggcgttacgg cgcggccagc
    27061 caaattcatc ggtcattggc tgaatatcag caaaacagcg gccttttggg gtgagaaaac
    27121 gcatcgcgtg ccacggtgta gtgtgcggca gaacattctc gaccaggccg accgactgca
    27181 tggtgcgcag cgcctcgtca tcaataccaa tcgcacgcgg gtagtcgatc aacttatcga
    27241 gtttctccac caccagcacg tcaatgccca tctgaccgag atagttcgcc atcatcagcc
    27301 caaccggacc ggcaccagcg atcgccacct gaacgctatg gttaacagca ggctggatgt
    27361 cagggtgttg tattgccatt tctgcacaac gttgttttaa ctatagaaat gtcaattaat
    27421 atgcagaaca atgagcagac ggaatacaaa accgtgcgcg gcttaacccg cggtctaatg
    27481 ttattaaata tgttaaataa acttgatggc ggtgccagcg tcgggctgct ggcggaactc
    27541 agcggcctgc atcgcaccac tgtgcggcga ctgctggaga cgctgcagga agagggatat
    27601 gtccgccgta gcccctccga tgacagtttt cgtctgacca tcaaagtgcg gcaattaagc
    27661 gaaggatttc gtgacgaaca gtggatttct gcactggcgg ccccgctgct gggcgatctg
    27721 ttgcgcgaag tggtatgacc aacagatgtg tccaccctgg atgttgatgc aatggtggta
    27781 cgcgaaacca cccaccgttt cagccgctta tcctttcacc gggcaatggt cgggcgacgt
    27841 ttaccgctcc tgaaaaccgc ctcgggcctg acctggctgg ccttttgccc ggaacaagaa
    27901 cgcaaggaat taatcgaaat gttagccgcc cgccccggtg atgactatca actggcacga
    27961 gaaccgttaa agctgcaagc cattctggcg cgcgcgcgca aagagggtta cggacagaac
    28021 taccgcggct gggatcagga ggagaagatc gcctctatcg ccgtaccgct gcgcagtgaa
    28081 caacgggtga ttggctgtct gaatctggtg tatatggcga gcgcaatgac cattgaacag
    28141 gcagcggaaa agcatcttcc ggcgctacaa cgggtagcaa aacagatcga agaaggggtt
    28201 gaatcgcagg ctattctggt ggccggacgg cgaagcggcg tgcatttacg ttgacaccat
    28261 cgaatggcgc aaaacctttc gtggtatggc atgatagcgc ccggaagaga gtcaattcag
    28321 ggtggtgaat gtgaaaccag taacgctata cgatgtcgca gagtatgccg gtgtctctta
    28381 tcagaccgtt tctcgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa
    28441 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc
    28501 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc
    28561 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc
    28621 gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca
    28681 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga
    28741 agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa
    28801 cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt
    28861 gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg
    28921 tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga
    28981 aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat
    29041 cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat
    29101 taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga
    29161 agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg
    29221 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca
    29281 gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc
    29341 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
    29401 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
    29461 ctttacactt tatgcttccg gctcgtatgt tgcgtgaaat tgtgagcgga taacaatttc
    29521 acacaggaaa cagctatgac catgattacg gattcactgg ccgtcgtttt acaacgtcgt
    29581 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
    29641 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
    29701 aatggcgaat ggcgctttgc ctggtttccg gcaccagaag cggtgccgga aagctggctg
    29761 gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct caaactggca gatgcacggt
    29821 tacgatgcgc ccatctacac caacgtgacc tatcccatta cggtcaatcc gccttttgtt
    29881 cccgcggaga atccgacggg ttgttactcg ctcacattta atattgatga aagctggcta
    29941 caggaaggcc agacgcgaat tatttttgat ggtgttaact cggcgtttca tctgtggtgc
    30001 aacgggcgct gggtcggtta cggccaggac agccgtttgc cgtctgaatt tgacctgagc
    30061 gcatttttac gcgccggaaa aaaccgcctc gcggtgatgg tgctgcgctg gagtgacggt
    30121 agttatctgg aagatcagga tatgtggcga atgagcggca ttttccgtga cgtctcgttg
    30181 ctgcacaaac cgagcacaca aatcagcgat ttccatgttg ccactcactt taatgatgat
    30241 ttcagccgcg cggtactgga ggcagaagtt cagatgtacg gcgagctgcg cgatgagctg
    30301 cgggtgacgg tttctttgtg gcagggtgaa acgcaggtcg ccagcggcac cgcgcctttc
    30361 ggcggtgaaa ttatcgatga gcgtggtggt tatgccgatc gcgtcacgct acgtctgaac
    30421 gtcgaaaacc cggcgctgtg gagcgccgaa atcccgaatc tctatcgtgc ggtggttgaa
    30481 ctgcacaccg acgacggcac gctgattgaa gcagaagcct gcgatgtcgg tttccgcgag
    30541 gtgcggattg aaaatggtct gctgctgctg aacggcaagc cgttgctgat tcgcggcgtt
    30601 aaccgtcacg agcatcatcc tctgcatggt caggtcatgg atgagcagac gatggtgcag
    30661 gatatcctgc tgatgaagca gaacaacttt aacgccgtgc gttgttcgca ttatccgaac
    30721 catccgctgt ggtacacgct gtgcgaccgc ttcggcttgt acgtggtgga tgaagccaat
    30781 attgaaaccc acggcatggt gccaatgaat cgtctgaccg atgatccgcg ctggttaccc
    30841 gcgatgagcg aacgcgtaac gcgaatggtg cagcgcgatc gtaatcaccc gagtgtgatc
    30901 atctggtcgc tggggaatga gtcaggccac ggcgctaatc acgacgcact ctatcgctgg
    30961 atcaaatctg tcgatccatc ccgcccggtg cagtatgaag gcggcggagc cgacaccacg
    31021 gccaccgata ttatttgccc gatgtacgcg cgcgtggatg aagaccagcc cttcccggct
    31081 gtgccgaaat ggtccatcaa aaaatggctt tcgctacctg gagagacgcg cccgctgatc
    31141 ctttgcgaat acgcccacgc gatgggtaac agtcttggcg gcttcgctaa atactggcag
    31201 gcgtttcgtc agtacccccg tttacagggc ggcttcgtct gggactgggt ggatcagtcg
    31261 ctgattaaat atgatgaaaa tggcaatccg tggtcggctt acggcggtga ttttggcgat
    31321 acaccgaacg atcgccagtt ctgcatgaac ggtctggtct ttgccgaccg cacgccgcat
    31381 ccagcgctga cagaagcaaa acaccagcag cagtttttcc agttccgttt atccgggcaa
    31441 accatcgaag tgaccagcga atacctgttc cgtcatagcg ataacgagct tctgcactgg
    31501 tcggtggcgt tggatggcaa gccgctggca agcggtgaaa tgcctctgga tgttgctcca
    31561 caagataaac agttgattga actgcctgaa ctaccgcagc cggagagcgc cggacaactc
    31621 tggcttacgg tacacgtagt gcaaccgaac gcgaccgcat ggtcagaagc cggacacatc
    31681 agcgcctggc agcagtggcg tctggcggaa aacctcagcg tgacactccc cgccgcgccc
    31741 cacgccatcc cgcaactgac caccagcgaa acggattttt gcatcgagct gggtaataaa
    31801 cgttggcaat ttaaccgcca gtcaggcttt ctttcacaga tgtggattgg cgctgaaaaa
    31861 caactgctga cgccgctgcg cgatcagttc acccgtgcac cgctggataa cgacattggc
    31921 gtaagtgaag cgacccgcat tgacccgaac gcctgggtcg aacgctggaa ggcggcgggc
    31981 cattaccagg ccgaagcagc gttgttgcag tgcacggcag atacacttgc cgacgcggtg
    32041 ctgattacga ccgctcacgc gtggcagcat cagggaaaaa ccttatttat cagccggaaa
    32101 acctaccgga ttgatggtag tggtcaaatg gcgattaccg ttgatgttga agtagcgagc
    32161 gatacaccgc atccggctcg gattggcctg acctgccagc tggcgcaggt agcagaacgg
    32221 gtaaactggc tcggattagg gccgcaagaa aactatcccg accgccttac tgccgcctgt
    32281 tttgaccgct gggatctgcc attgtcagac atgtataccc cgtacgtctt cccgagcgaa
    32341 aacggtctgc gctgcgggac gcgcgaattg aattatggcc cacaccagtg gcgcggcgac
    32401 ttccagttca atatcagtcg ctacagccaa caacaactga tggaaaccag ccatcgccat
    32461 ctgctgcacg cggaagaagg cacatggctg aatatcgacg gtttccatat ggggattggt
    32521 ggcgacgact cctggagccc gtcagtttca gcggaattcc agcttagcgc cggtagctac
    32581 cattaccagt tggtctggtg tcaaaaataa taataaccgg gcaggccatg tctgcccgta
    32641 tttcgcgtaa ggaaatccat tatgtactat ttaaaaaaca caaacttttg gatgttcggt
    32701 ttattctttt tcttttactt ttttatcatg ggggcctact tcccgttttt cccgatttgg
    32761 ctacatgaca tcaaccatat cagcaaaagt gatacgggta ttatttttgc cgctatttct
    32821 ctgttctcgc tattattcca accgctgttt ggtctgcttt ctgacaaact cgggctgcgc
    32881 aaatacctgc tgtggattat taccggcatg ttagtgatgt ttgcgc
//
LOCUS       DAKISA010000041        31832 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.041, whole genome shotgun sequence.
ACCESSION   DAKISA010000041 DAKISA010000000
VERSION     DAKISA010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31832
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.041"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            1..467
                     /locus_tag="OVW45_002000"
                     /pseudo
     CDS             1..467
                     /locus_tag="OVW45_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            570..1073
                     /locus_tag="OVW45_002001"
     CDS             570..1073
                     /locus_tag="OVW45_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HCV5526455.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTPAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1140..2597)
                     /gene="nuoN"
                     /locus_tag="OVW45_002002"
     CDS             complement(1140..2597)
                     /gene="nuoN"
                     /locus_tag="OVW45_002002"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCV5526456.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPMGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLYFYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSASGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2604..4133)
                     /gene="nuoM"
                     /locus_tag="OVW45_002003"
     CDS             complement(2604..4133)
                     /gene="nuoM"
                     /locus_tag="OVW45_002003"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCV5526457.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     AEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     EIASQELPGMSLRELFMILLLVVLLVLQGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4297..6138)
                     /gene="nuoL"
                     /locus_tag="OVW45_002004"
     CDS             complement(4297..6138)
                     /gene="nuoL"
                     /locus_tag="OVW45_002004"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCV5526458.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAIVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVRPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6135..6437)
                     /gene="nuoK"
                     /locus_tag="OVW45_002005"
     CDS             complement(6135..6437)
                     /gene="nuoK"
                     /locus_tag="OVW45_002005"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCV5526459.1"
                     /translation="MIPLQHGLILAAILFILGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6434..6988)
                     /gene="nuoJ"
                     /locus_tag="OVW45_002006"
     CDS             complement(6434..6988)
                     /gene="nuoJ"
                     /locus_tag="OVW45_002006"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCV5526460.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7000..7542)
                     /gene="nuoI"
                     /locus_tag="OVW45_002007"
     CDS             complement(7000..7542)
                     /gene="nuoI"
                     /locus_tag="OVW45_002007"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCV5526461.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPIYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7557..8534)
                     /gene="nuoH"
                     /locus_tag="OVW45_002008"
     CDS             complement(7557..8534)
                     /gene="nuoH"
                     /locus_tag="OVW45_002008"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCV5526462.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8531..11257)
                     /gene="nuoG"
                     /locus_tag="OVW45_002009"
     CDS             complement(8531..11257)
                     /gene="nuoG"
                     /locus_tag="OVW45_002009"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCV5526463.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLCEGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11310..12647)
                     /gene="nuoF"
                     /locus_tag="OVW45_002010"
     CDS             complement(11310..12647)
                     /gene="nuoF"
                     /locus_tag="OVW45_002010"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCV5526464.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12644..13144)
                     /gene="nuoE"
                     /locus_tag="OVW45_002011"
     CDS             complement(12644..13144)
                     /gene="nuoE"
                     /locus_tag="OVW45_002011"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCV5526465.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13147..14949)
                     /gene="nuoC"
                     /locus_tag="OVW45_002012"
     CDS             complement(13147..14949)
                     /gene="nuoC"
                     /locus_tag="OVW45_002012"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCV5526466.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15043..15705)
                     /gene="nuoB"
                     /locus_tag="OVW45_002013"
     CDS             complement(15043..15705)
                     /gene="nuoB"
                     /locus_tag="OVW45_002013"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCV5526467.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15721..16164)
                     /gene="nuoA"
                     /locus_tag="OVW45_002014"
     CDS             complement(15721..16164)
                     /gene="nuoA"
                     /locus_tag="OVW45_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCV5526468.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16793..17731)
                     /gene="lrhA"
                     /locus_tag="OVW45_002015"
     CDS             complement(16793..17731)
                     /gene="lrhA"
                     /locus_tag="OVW45_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCV5526469.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPIEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18651..19868
                     /gene="alaA"
                     /locus_tag="OVW45_002016"
     CDS             18651..19868
                     /gene="alaA"
                     /locus_tag="OVW45_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCV5526470.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            19952..20551
                     /gene="yfbR"
                     /locus_tag="OVW45_002017"
     CDS             19952..20551
                     /gene="yfbR"
                     /locus_tag="OVW45_002017"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCV5526471.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20610..22442)
                     /locus_tag="OVW45_002018"
     CDS             complement(20610..22442)
                     /locus_tag="OVW45_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCV5526472.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(22529..23178)
                     /gene="hxpA"
                     /locus_tag="OVW45_002019"
                     /pseudo
     CDS             complement(22529..23178)
                     /gene="hxpA"
                     /locus_tag="OVW45_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416796.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
     gene            complement(23189..23683)
                     /locus_tag="OVW45_002020"
     CDS             complement(23189..23683)
                     /locus_tag="OVW45_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCV5526473.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(23766..24221)
                     /locus_tag="OVW45_002021"
     CDS             complement(23766..24221)
                     /locus_tag="OVW45_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCV5526474.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            24559..25761
                     /gene="ackA"
                     /locus_tag="OVW45_002022"
     CDS             24559..25761
                     /gene="ackA"
                     /locus_tag="OVW45_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCV5526475.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            25836..27980
                     /gene="pta"
                     /locus_tag="OVW45_002023"
     CDS             25836..27980
                     /gene="pta"
                     /locus_tag="OVW45_002023"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCV5526476.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28170..29690
                     /gene="yfcC"
                     /locus_tag="OVW45_002024"
     CDS             28170..29690
                     /gene="yfcC"
                     /locus_tag="OVW45_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCV5526477.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29723..30265)
                     /gene="yfcD"
                     /locus_tag="OVW45_002025"
     CDS             complement(29723..30265)
                     /gene="yfcD"
                     /locus_tag="OVW45_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCV5526478.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30323..30874)
                     /gene="yfcE"
                     /locus_tag="OVW45_002026"
     CDS             complement(30323..30874)
                     /gene="yfcE"
                     /locus_tag="OVW45_002026"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCV5526479.1"
                     /translation="MKLMFASDIHGALPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(30930..31574)
                     /gene="yfcF"
                     /locus_tag="OVW45_002027"
     CDS             complement(30930..31574)
                     /gene="yfcF"
                     /locus_tag="OVW45_002027"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCV5526480.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLKPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RRIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
BASE COUNT         7943 a         8719 c         8008 g         7162 t
ORIGIN      
        1 cgacaatatt gataatctga ataggtccgt agagtatata aaagagttct ttgtcagtag
       61 cggtgccaga gttaccgcgc agggcgtccc cgattggcta ttacgactct gcgcctggta
      121 gccaggatta cccttacccg caatgtcctg gctttatccc gatcgggggg actttattgc
      181 ctggtcggca gaatacagga tatcaacgcc gttcgtcagg taaaaacggc attgttatca
      241 tcccgggatt tatctgttta ttctatgaat gccccatggt ttattcccgg tattgatttc
      301 tctgaccacc tgaattattg gcaacacgat attcccgccg taatgattac tgacaccgct
      361 ttttatcgta ataaacaata ccacttgccc ggtgatacag cagacagatt gaattatcag
      421 aaaatggctc agatggtgtt ataaccttgt tacacaacag taaataataa aaatttctcg
      481 aggcgggtcc agaaacagcg acatgtacat tttattttcc ctcgtctgtt ttaagatgcg
      541 aaacataaaa ttcaatggag gaaaaataaa tgggaatgat tggttatttt gcagaaattg
      601 actcagaaaa gattaaccaa ctgcttgagt ctactgaaaa acctttgatg gataatatac
      661 atgacacgct ttcaggatta cgccgactgg atattgataa acgctgggat tttttacact
      721 ttgggctgac aggcacccct gcttttgatc ccgcgaagaa tgatccgctg agtcgtgccg
      781 tgcttggtga acacagcctc gaagatggca tagatggctt cctgggattg acctggaatc
      841 aggagttagc cgcgacaatc gaccgactgg aatcactgga tcgaagtgag ctgcgtaaac
      901 agttttccat taagcgactc aatgaaatgg agatttatcc gggagtgacc ttttcagaag
      961 agttagaggg gcaactcttt gcttcgatca tgcttgatat ggaaaagctg attagcgcgt
     1021 atcgacgtat gctgcgacaa gggaatcatg cgctgacggt tattgttggg tgatggtgtc
     1081 agggcaccaa aaaaaaccgc cgaacatgtc ggcggctttc tgacttacaa actgatagct
     1141 tacatcagcg gcattgctaa ccgcacaatg ctaatcagcg gttgtggcca tacacccagc
     1201 accagtacca acagtgcaga gatcagcacc acgataccgc ctgcgctgta ctgccagttt
     1261 gacggtgcat cgcgacccgg ttgttccggg gcgtgcaggt acaggctcac cgccacgcgc
     1321 aggtagtagt aaaggccgct tgccgaaccg acaaccacgg cgcccaccag ccaccacaag
     1381 tgtgcctgga caccgaccgc cagcacgtag aacttaccaa taaagcccag cgtcatcgga
     1441 ataccggcca gagacagcat catcaccgtc atcaccgccg cgaggatcgg gcgatgccag
     1501 aacagtccgc ggtaaaagta cagggaatca gcatccgggc cacgatacgg gctggacatc
     1561 aggctgacca cgccgaacgc gccgaggctg ctaaacagat aaccggccag gtaaacccct
     1621 accgcttcca tcgacatctc gccagtttgc agcgcaatca gcgccaccag cagatagccg
     1681 aggtgagaga tagatgagta accgagcaga cgtttgatat tggtctggct cagcgccatc
     1741 aggttaccga agatgatgga ggcaaaggcg ataatcgcca gcaccacgcg aatcgcttcg
     1801 ctgtcaccca tcggtgcgta gaggaacaga cgcatcacca caccgaagat agcgattttg
     1861 ctcgccgtcg ccaggaaagt ggaaaccggc gcaggcgcgc cctggtatac gtctggcgtc
     1921 cacaggtgga acggcaccag agagagtttg aagccgaggc caacaatcat caggccgaaa
     1981 cctgccagca acagcggctc gttgagcata ccgtcgccaa ggtttttacc caacgcgaca
     2041 aacgacaggt cgccagactg cgcatacacc agcgccatac caaacagcag gaaagaagac
     2101 gctgcggcag aaaggatggt gtatttgata ctggcttcca gtgaacgttt ctggcggaaa
     2161 gcgtaaccga ccaggccaaa cagcggcaaa gagatcagtt cgataccgag gaacagagac
     2221 gccagatggt tggcattcgc cagcaggatc ccgcccagcg cggcaattaa caccaacagg
     2281 tagaactcgt ccttgttgtc gttatagcct tcaagccacg ggtaggcgaa agtacaggtg
     2341 gcgaggctcg ccaacaatac cagcccggtg taaagcatgg cgaaaccatc aacgcgcatc
     2401 agcggcgtaa cgtccatagc gcctgcctgg ccgacaaacc agagcgaaac cagcgccgcg
     2461 ttaagcccaa taaccgagag cgtagcgttg aggaaatgat tgcgtcgcca cgcaatggag
     2521 agcatcacaa ccaccaccgt caagccgacg atcagcaacg gtagcagtgc gatcaggttt
     2581 tgtggagtta ttgtcatggc gatttacggc cttgtagtag taacggaatt aacaaaccac
     2641 tgctggatat tgccaatcgc ggagtgcgag gtatccagaa tcggctgcgg atagaagccc
     2701 tgcagtacca gcagcaccac cagcaacagg atcataaaca gctcacgcag cgacatccct
     2761 ggcagttcct ggctggcaat ctcgcttttc gctttaccga agtaagcgcg atgcaacatc
     2821 gccagcgaat aaacagatgc aaagaccagc ccaaaggtag agataacggt aatcaccggg
     2881 acaacctgga agctgccgaa cagaatcata aattcgccga cgaagttacc ggtgccaggc
     2941 atcccaagcg ttgccaccgc aaagaacagc gacagtgctg gcagccattt catcttgctc
     3001 cacagaccgc ccatcatgcg catgtcgcgg gtatggatac gttcataaag ctgaccacaa
     3061 agaataaaca gacccgccgc cgacaagccg tgcgcaatca tctggattac cgcgccctgg
     3121 taggccaact ggctgccggt gtagatagca atcagcacga agcccatgtg ggaaaccgag
     3181 gtgtaggcga tcagacgttt gatatcggtc tgggcgaagg ccatccacgc accgtagaag
     3241 atgccgataa cacccagcca catagcaatt ggcgcgaact ctgccgacgc gttcgggaac
     3301 agcggcaggg agaaacgcag caaaccgtaa gcggcagttt tcagcaagat ccccgcgagg
     3361 tcaacggaac cggcggtcgg agcctgggag tgcgcatccg gcagccagcc atgcagcgga
     3421 accaccggca ttttgactgc gaaggcgatg aagaagccca gcattaacag gtattccgca
     3481 ccactggaca ttggcgtatt cagcagctct tcatagttga aggtccagac gccggtcgca
     3541 ttgtagtgaa caaaaaccag cgccaggatg gcaatcaaca tcaccagacc actcgcctgg
     3601 gtgtaaatga agaacttggt tgccgccgtg atacgcgttt taccgtcaga ggctttatgc
     3661 ccccacagtg cgatcaggaa gtacatcggc accagcatca tttcccagaa gaagaagaac
     3721 aggaacatgt cgatggcaag gaacacgccg ataacgccgc ccaggatcca catcaggttg
     3781 aggtggaaga agccctgata tttttcgatc tctttccacg aacatagtac cgccagcaca
     3841 ccgagcagac cggtcagcac gaccatcagc agcgacagcc cgtcaatggc gagatgaata
     3901 gagataccaa aacgcgggat ccacggcatg tcgaattcag actgccactg cggaattccg
     3961 gcggattgcg tcagtgaata accgccctgc aaccacagtt gcagcgacag cgccagcgtc
     4021 aatcccatgg tgatcagcgc gatccagcgt ggcaccttga cgccaaagcg ttcggtctgc
     4081 cagcacagga agccgccaat aaagggaatt aatattagcc agggtagtaa catggcgatc
     4141 tttattcctt gtaaaagtcc cgtcaggacc ggattttcaa cgaattctca cgacagaaaa
     4201 ttctcttcgg actgggggta gtgtcggatg caccgcgaag gcggcttatc cgacctacgt
     4261 tcttatatgc ccaggggcaa tcccacaatc cttaactcaa cgcagtacca tcaacagtgc
     4321 cagcaccacg accgcaccga tgctcatgga tgccacatac cagcgcagat agccgttctc
     4381 acttaacagc agacctttac ctgcaaagcg ggaaaggaca gccggaatgt tcatcattga
     4441 gttcagcgga tcgcgtttca gcaaccaggc aatacccagg aacggcctga cgaacacttt
     4501 gtcgtacagc cagtcaaagc cccaggcgtt gtaccaccag gtactcagca ggcggcccgg
     4561 cgcactgttg gcgatggagg tcaccagagt acgtttaccc agccacagcc aagctgccag
     4621 cagaatgccg acaatcgcga ccacgccaga ggtaatttcc agggtcaaca tgctgccgtg
     4681 cgccagttcc gtcgtttgcg gaagcacgcc ctgcagcggc ggtacaatca gtgcgccaac
     4741 gaaggtggaa aggatcagca gcacaatcag cggcaggctg tgagttaccc ctttcacggc
     4801 gtgagcgtga atttgttctt ttccgtggaa gacgatgaaa atcatacgga aggtgtagag
     4861 cgaggtcata aacgcaccga ccagacctgc caccatcaga ttgatatgac cattcgccat
     4921 cgcacccgcg aggatctcat ccttactgaa gaagcccgca gtgaccagcg gcagtgccga
     4981 cagtgctgcg ccgcccacca ggaagcagag ataaaccagc ggaatagatt tacgcagacc
     5041 gcccatcttg aagatgttct gttcgtgatg gcaggccaga atgacggaac cggatgccag
     5101 gaacagcagc gctttaaaga acgcgtgagt catcaagtgg aaaatcgccg catcccatgc
     5161 ctgcacgcca agcgcgagga acatgtagcc aatctggctc atggtagagt aagcgagaac
     5221 acgtttgatg tcggtctgta ccagcgcggc aaaaccggcc agcagcagcg taaccgcccc
     5281 gacaatgccc accagatgca gaatttccgg cgtcatcagg aacaggccgt gggtacgggc
     5341 gatcaggtag acacccgcgg ttaccatggt tgcggcgtgg atcagcgcgg agacaggcgt
     5401 cgggcccgcc atcgcgtcgg caagccatgt ctgcaacggc aactgcgcag atttaccgac
     5461 cgcaccgccc agcagcatca gcgtcgccca catcagcatg ttattgccgt cagcaaagtg
     5521 cgctggtgcc agttccacca tttcgcggaa gttcagggtg cccagttcgt tgtaaagaat
     5581 gaacagtgcg aaagcgagga aaacgtcacc cacacgggtc acgacgaacg ctttcattgc
     5641 cgctgcgcca ttcttcggat cggtgtaata gaaaccgatc agcagatagg agcacaggcc
     5701 cacgccttcc cagccgaggt acatcagcag caggttgtcg gcaagcacca gaactaccat
     5761 gctggcgatg aacaggttgg tgtaggcgaa gaagcgagag tagccctctt caccgcgcat
     5821 gtaccaggag gcgtacatgt gaataaggaa acccacacca gtgaccaccg agagcatggt
     5881 cagcgacagg ccgtccagca ccaggttaaa accgatgtta aagtcgccta ccgacatcca
     5941 cgtccacagc ggctggctgt atgcctgctc gccgttagcg aagaaatcaa cgccgataaa
     6001 ggcggttacc agcgccgcca ggcccacaga gccaacgccg acgatcgccg aaacgttttc
     6061 agaccagcgc ccacgggaga acgccagcag gacgaagcca atcaatggca aaataatggt
     6121 taaggcaagc atgttcatcc gcgcatctca cttactgaat cgatgttcag gttctggcga
     6181 cgacggtgaa gttgcagcag cagcgcaagg ccgatactcg cttctgccgc cgcgaggctg
     6241 atggcgagaa tgtacatcac ctgaccgtcg gtctgacccc agtagcttcc ggcgacaaca
     6301 aaggccagcg cggaggcgtt aatcatgatt tccagaccaa tcagcataaa cagcagattg
     6361 cgacggataa ccagaccggt taagccaaga atgaataaga ttgccgcgag gatcagtcca
     6421 tgttgtaagg ggatcatgcg tgctcctccg tttttctttt cgcgctgtcg tctttacgat
     6481 tgctcagcac ttcacccgca cgctcttcac gaccgacgtg gaaggccaca accagacctg
     6541 cgagcagcag catagaagcc agttccaccg ccagtacgta aggcccgaac agcgtaatac
     6601 ccactgcttt agcactgatt ggcgtaccgt cgataccctg atcgttaaca ccgaggatgg
     6661 cgtaaacaat caccaccagc atgatggccg acaaaattgc cggaccaatc cacacctgcg
     6721 gtttcagcca ctggcgttcc tgttcgattt ctgaaccgcc caggttgagc atcatcacca
     6781 cgaacacgaa cagcaccata atggcacccg cgtagacgat aatttccagc gcaccggcga
     6841 agtaagcgcc cagtgagaag aacaccccgg aaatcgccag cagcgaaata atcaggtaca
     6901 gcagtgcgtg taccggattg gtatgggtga tcactcgcaa ggtcgcaagt atggctatca
     6961 ggccacagat ataaaaagcg aactccattg cccctctcct tacggtaaca ggctcttgac
     7021 gtcgataggc ttggcttcgt tctctgcttc gcccttatct ttgccgtcga ttgccatacc
     7081 tgccatccgg tagaagttat attccgggta tttgcccgga ccggagatca gcagatcctc
     7141 tttttcgtaa accagatcct ggcgcttgta ttcccccatt tcgaaatccg gggttaactg
     7201 aatcgccgtg gtcggacagg cttcttcgca cagaccacag aaaatgcagc gtgagaagtt
     7261 gatgcggaaa aattccggat accagcgacc gtctttggtt tctgcttttt gcagcgagat
     7321 acagccgacc gggcaggcta ccgcgcagag gttacaggct acgcaacgct cttcgccgtc
     7381 cgggtcgcgg gtcagaacga tacgaccacg ataacggggc ggcagataga tcggctcttc
     7441 cgggtacatt cgcgtttcgc gtttggcgaa cgcgtgcagg ccgatcatcc agatactacg
     7501 aacctgggtg ccgaaaccta ctaacaattc ttttaaggtc atggtcttat tgccccttat
     7561 tgcgcctgcc agagaatgac agccgccgtt accagcaagt tgatcagcgt cagcggcagg
     7621 cagattttcc agccgaagga cattacctgg tcataacgcg gacgcggtaa cgacgcacga
     7681 atcaaaatga acatcatcat aaagaacgcg gttttcagcg cgaaccagat gaatggcggt
     7741 aacaacgggc cttgccagcc accgaagaac agcgtcacca tcaatgcaga gatggtcaca
     7801 atcccgatgt actcacccac gaagaacaga ccgaacttca taccggaata ttcaatgtgg
     7861 taaccatccg ccagttcctg ctcggcttcc ggctggtcaa acgggtgacg gtgacatacc
     7921 gccacgcccg cgatggcaaa ggtaataaaa ccaaagaatt gcgggataac gttccacaca
     7981 tgcgcctggc tgttgacgat gtcggtcatg ttgaatgaac cggcctgcgc caccacgccc
     8041 atcagggaaa gcccgaggaa cacttcgtag ctcagggtct gcgcagaagc acgcatcgca
     8101 cccagcaacg agtatttgtt gttacttgac cagcccgcaa acagcaccgc gtaaaccgcc
     8161 agacctgcca tcatcaggaa gaacaaaatc ccgatgttca ggtcggcaac cacccaaccc
     8221 ggactgactg gcacaatcgc aaaggccagc agcagcgagg taaaggcaat catcggtgcc
     8281 agggtaaaga tgacgcgatc cgagaatttc gggatccagt cttctttaaa gaacattttg
     8341 atcatgtccg caaccagctg gagcgaaccg ccccagccaa cacggttagg tccgtaacgg
     8401 ttctggaaca gacccagcag gcgacgttcg ccaaagctca tgaatgcccc gcaggtgaca
     8461 accaccagca ggatcaccac cgctttgagg atggtcagca ggatctcaat cagttccggt
     8521 gatatccaac tcattgttgt gcctccttga gatcctcaag atgcgcgcca gccagcaccg
     8581 gagcaatacc ggacataccc atcggcaagc ccacctgccc tgccgtcagc ccttcagcga
     8641 tttcaaccgg cagcgtgacc gtattgccat cgtaactaaa ggagacgtgt gtacctgcgt
     8701 tcacacctaa cttcgcggca tccgctgggt tgagtttgat gtacggctgc ggcatacggc
     8761 tctggaagac cggagcacgc tgtgacaatt catcgctgcc aaacaggtgg taatacggcg
     8821 cgatacgcca tttcccgtcc tgcggctgga agcgtgccgg tacgctggtg aagtaatcca
     8881 gaccattttc gctggtttca aacagacgca cgcccggatc gccaaagcgc agtttgccgc
     8941 ccacttcgtc ctggaatttg ttccacgcct gcggggagtt ccagcccggc gcccaggcaa
     9001 acggcacttg cgaacggtgc gcagtcggct ggttgttacc ttccatcgag aaggtgaaca
     9061 tggtgtcaat atcctgcggc tgacgcggct catgaacgct gatattggcg cgcatggcgg
     9121 tacgaccgct gtaacggtgc ggttcacggg ccagtttctg cccacgaata cggaatgtcg
     9181 catccggcgc agcatctttg atacctgcca gttccgggat tttcgccaca acagcgtcaa
     9241 tcacatggtc gagctgcgtc cagtccactt cacggctcag cagggtgctg tgcagcgagt
     9301 gtaaccagcg ccagctttcc agcatgacag ttttgctgtc gtaataagca ggatcgtaaa
     9361 cctggaagaa acgttgggcg cggccttcgt tgttgatcac cgtaccgtcg ctttcagcaa
     9421 aactggcagc agaaagtacc agatgggcgt tttccataat cgctgtgcgt tgatgatcaa
     9481 ccaccatcac cagcggcgct ttagccagcg cagcattcac gcgggtagcg gaagcgtgac
     9541 gatgcagatc gttttccagc accaccaccg cgtcggcgcg tccggtttcc agttcggtca
     9601 acgcttcttc aagcgaaccg ccacccataa tgcccagccc catgctgttg acggaacggg
     9661 caatcatggt gataccaacg tcagcgccgc gacctttcag ggctttcgcg acgttagccg
     9721 cagcctgaat cacctctatg ctaccggcgt tcgtcccgga gataatcaac ggtttcttcg
     9781 cacctgccag tgcctgcacg atgacgtcga ttttgctttg cagctcaggt tcgataccgt
     9841 caaccgctgg cgcagagtta tccagcgcat gggcgatggc aaaacctaaa cgcgcctgat
     9901 cttcaaccgg tgcgcggtaa gtccacgccg cgatatcatc cagacgggtg tcatcaacgt
     9961 tggtaacaaa cagcggatgc ttcgcacgtt gaccgatgtt gaggattgcc gcaatctgcc
    10021 agtcagccac tttctgtgct gccgccattt cgcgcgcttt acctttcaca gcctgacgca
    10081 ctgccagcgc gacgcgcgcg ccggtctggg taacgtcttc gcccagcacc agtaccgcat
    10141 cgtaagattc gatttcgcgc agagccggag tataaatgcc gccttcacac agcactttca
    10201 gcgccagttg cagacgttcc tgctcaccgt gagcgatacc ggtgtagaag ttttcttcgc
    10261 ccaccagttc acgcagcgca aagttgcttt ccacgctggc acgcggagaa ccaataccga
    10321 tcactttctt cgactgacgc agaatatctg ccgcgccctg cattgcctgt tcggcgttga
    10381 gggtaatgaa atcatcgcca cgacgctgta ctggctgacg cggacgatcc ttcaggttga
    10441 cgtaaccgta accgaaacga ccacggtcgc agaggaagta gtggtttacc gtaccgttgt
    10501 aacggttttc gatacgacgc agttcgccgt aacgttcacc ggggctgatg ttacagccga
    10561 tggaacattg ctggcagatg ctcggcgcaa actgcatatc ccatttacgg ttgtaacgct
    10621 cggagtgcgt tttgtcggta aatacgccgg tcgggcaaat ttcgaccagg ttaccggaaa
    10681 attcgctttc cagcgtgccg tcttccgggc gaccgaagta gacgttgtcg tgcgcaccgt
    10741 aaacgcccag atctgtaccg tcagcgtaat ctttgtagta acgcacacag cggtagcagg
    10801 cgatgcagcg gttcatttcg tgagagatga atggccccaa atcctgatta cggtgggtac
    10861 gtttggtgaa acggtagcga cggaagctgt gtccggtcat cacagtcata tcctgaagat
    10921 ggcagttacc gccttcttca cataccggac agtcgtgcgg gtggttggtc attaaccact
    10981 cgaccacact ttcacggaac tgtttcgctt cttcgtcgtc aatggaaata aaggtgccat
    11041 cggaagccgg tgtcatacag gacatcacca ggcgaccacg cgtgtcttcc gcgttttggt
    11101 attgcttcac cgcacactgg cggcaagcac cgacgcttcc cagcgccgga tgccagcaaa
    11161 agtaaggaat atcaaggccc agagacagac aagcttccag caggttgtcc gctccgttga
    11221 cctcgtattc tttgccgtct acatgaattg tagccattag catgcttcca gttttctcgg
    11281 tcagggaccg agcgttaatc gaaattcggt taccagcgct ctttcagcag gttcggctga
    11341 atcccattaa tcaaatgggt attgctgaac ggctgtttga ttcccgcctc aaattcttcg
    11401 cggaaatatt tgatggcgct ctgtaacggc tccactgcac caggtgcgtg ggcacagaaa
    11461 gttttacccg ggcctaagaa tcgacacagt tgctcaagtg tttcgatatc gcccggctgg
    11521 ccttcgccac gctccagcgc acgcagaatt ttcacgctcc acggcagacc gtcgcggcac
    11581 ggcgtacacc agccgcagga ctcacgggcg aaaaactctt ccaggttacg caccagcgac
    11641 accatgttga tctcatggtc aaccgccatc gccagcgccg tacccagacg gctgcccgct
    11701 ttaccgatac tttcgaattc catcggcaga tcaaggtgcg cttcggtcag gaagtcagtc
    11761 cccgcgccgc ctggctgcca ggctttaaat ttcagaccat cacgcatacc accggcgtaa
    11821 tcttcgagga tctcgcgtgc ggtggtgccg aacggcagtt cccacagtcc cggatttttc
    11881 acccgaccgg agaagcccat cagcttggtg ccagcatctt tacttttcga gatgttctga
    11941 taccactcca cgccgttagc gaggatcgcc ggaacgttac acagggtttc gacgttgttg
    12001 acacaggtcg gtttgcccca tacgccggag gttgccggga aggggggttt cgagcgtggg
    12061 ttagcacgac gcccttccag ggagttgatt aacgctgttt cttccccgca gatgtagcgc
    12121 cctgccccgg tatggacgaa cagttcgaag tcgaaacctg ttcccataat gtttttgcca
    12181 agcaagcccg cttcagtggc ttcggcaatg gcgcggcgca gatttactgc cgcttcgata
    12241 tattcgccac gcaggaagat gtagccacgg taagctttca gcgcaaacgc ggagatgagc
    12301 ataccttcca ccagcaggtg cggcagttgc tccatcaaca ggcggtcttt ataggtgccc
    12361 ggctccattt catcggcgtt acacagcagg taacggatgt tcatggattc gtctttcggc
    12421 atcaggctcc acttcaggcc agtcgaaaag cccgctccgc cgcgcccttt cagaccagcg
    12481 tcttttacct gattaacgat ttcgtccgga gacagcccgg tcagcgcctt acgcgcccct
    12541 tcgtaaccgt ttttgctgcg gtattcgtcc agccacactg gctgtttgtc atcgcgcaga
    12601 cgccaggtca gcggatgcgt ttcgggagta cggataatgt ttttcattta taccgctcca
    12661 gcagttcagg gatcgcttcc ggggtcagat gcgcgtgagt gtcctcatcg atcatcatgt
    12721 ttggcccttt atcacagttc cccaggcagc aagttggcag cagcgtaaag cggccatcaa
    12781 atgtcgtttg ccctggtttg atgttcagct ttttctcgag cgccgcctga ataccctgat
    12841 acccgttgat atgacagacc acgctgtcgc aataacggat cacatggcga ccaaccggct
    12901 ggcggaagat ctgactgtag aacgttgcca caccttcgac gtcgcttgcc ggaataccca
    12961 gcacatcggc gatcgcgtgg atcgcaccat ccggcaccca gccacgctgc ttctgaacga
    13021 ttttcagcgc ttcaatggac gccgcacgcg ggtcttcgta gtggtgcatc tcgtgctcaa
    13081 tcgcttcacg ctctgccgca ctcagctcaa aagcctcggt ttgtggttgt tgattctcgt
    13141 gcataattag cggtccacat ctgacataac aaaatcgata ctgcccagat aaacaatcag
    13201 gtcagacacc aggctgccgc ggatcgccgc cggaatttgc tgcaaatgcg cataactcgg
    13261 cgtgcggata cgagtgcgat aactcatggt gctaccgtcg ctggtcaggt agtaactgtt
    13321 gatccccttg gtcgcctcaa tcatctggaa agattcattg gcaggcatca ccggccccca
    13381 cgacacttgc aggaagtggg tgatcagggt ttcgatatgt tgcagcgtgc gctctttcgg
    13441 cggcggcgtg gtcagcgggt gatccgcttt gaacgggcct tccggcatgt tgttgaggca
    13501 ctgctcaaga atgcgcagac tctggcgcag ctcttccact ttcagcatta cgcgggtgta
    13561 gcagtcagaa acaccaccac caaccgggat ttcaaagtcg aagttttcat agccagaata
    13621 aggacgcgcc ttacgcacgt cgaagtcgat cccggtagca cgcaggcccg cgccagtggt
    13681 gccccactcc agcgcctctt tcgcgccata ggcggcaacg ccctgggaac gacctttcag
    13741 aatggtattt tgcagcgccg ctttctcgta agacgccaga cgtttcggca tccagtcgag
    13801 gaactcacgc agcaggcgat cccagccgcg cggcaggtcg tgcgctacgc cgccaatacg
    13861 gaaccacgcc gggtgcatac ggaaacccgt gatcgcttcc accagatcgt aaattttctg
    13921 acgatcggta aaggcgaaga acacgggcgt cattgcgccg acgtcctgaa taaaggtcga
    13981 gatgtacagc aggtggctgt tgatacggaa cagttcggag agcataacgc gaatgacgtt
    14041 aacgcgatcc ggcacggtga tcccggccag tttctctacc gccagcacgt aaggcatttc
    14101 gttaacgcag ccgccgaggt attcaatacg gtcggtatac ggaatgtagc tgtgccaaga
    14161 ctggcgctcg cccattttct ccgcaccacg gtggtggtag ccgatgtctg gtacgcagtc
    14221 gacaatctct tcgccgtcga gttgtaaaac gatacggaaa gccccgtgcg ccgacgggtg
    14281 gttcggaccg aggttgagga acatgaagtc ctcgttttcg gtgccgcgct tcatccccca
    14341 ctcttccggt ttgaaggtca gggcttccat ctccagatcc tgtttggctt tggtcagctc
    14401 aaacggcgag aattcggtag cgcgcgccgg gtaatcttta cgcagcgggt gacctttcca
    14461 ggtttgcggc atcatgatgc ggcgcaggtt cgggtgaccg tcgaaagtaa tgccaaacag
    14521 atcccaggtt tcacgctcat accagttagc gttcgggaac agtttggtga aggtcggtac
    14581 gtgcaggtcg ttttctgcca gcgccacctt cagcatgatg tcgcggttac gatcgataga
    14641 aatcagatgg tagaaaacgg aaaaatccgc ggcaggtaac ccttcgcggt gtgtgcgcag
    14701 acgttcgtcc atgccgtgta agtcaaacag catgacgtaa ggtttcggca gtttctttaa
    14761 gaaatcgcca acttccagta attgttcacg cttgatccac acaacgggaa ccccggtgcg
    14821 agtcgcctga acagtaaagg catccggccc aaaacggttg cgcagttcgc caatcaccgg
    14881 atcatcaaga tgatcacggg tctgccaggc gggttcttgc gcggttaagt cggtcatatt
    14941 gttcaccatt gcaaatggtc cgtggtgact gtcggcgcag taagcttcgc gaaatagatt
    15001 taggaataag cgaagaaaaa tccctttgcc gacaggcgca aattaaatct cgtcaggtgt
    15061 acgcaggttg gttacggcaa tgcgttcacc gcgcttgcgt tcgcgctctg attgcatatt
    15121 ggcgcgataa acgccctgat cgccaaccac ccaggagagc ggacgacgtt ctttgccgat
    15181 agattcctgc aacagcatca gtgcctgcat gtacgcttca ggacgcggcg ggcagcccgg
    15241 gatatacaca tcaaccggga tgaatttatc gacgccctgc acaacggaat aaatatcgta
    15301 cataccacca gagttggcac aggcacccat tgagataacc cattttggtt ccagcatctg
    15361 gtcatacaga cgctgaataa ccggtgccat tttggtaaag caggttcctg caaccaccat
    15421 caggtcagcc tgacgcggcg aagcacgcaa tacttctgcg ccaaaacgcg ccacgtcatg
    15481 caccgcggta aacgaagtca ccatctcaac gtaacagcag gaaagaccga agttatacgg
    15541 ccaaattgag tttttacgac cccagttaac catgtcattg agcttgccca taaacacgtt
    15601 tttgttaact tcttgctcca gagggtcggt tacgatctcc tgcttttgca gggggtaacg
    15661 gtcgttctca ccgttgggat ctatgcgggt gagcgtataa tccatcttaa tgcctcgcgg
    15721 ttagcgttga cgattagcga tactgttcgt ttccgggttc atacgctcgc ggcgtgaacg
    15781 cgcgggcgtc cagtccagcg cgccaatacg caccagataa accagacctg ccagtaacac
    15841 aaaaataaaa attgcagctt ccacaaagcc tacccagccg ctttcgcgga tagaggttga
    15901 ccatgcgaac agatacagcg cttcaacgtc gaagataacg aagaacatgg ccaccagata
    15961 aaacttggcg gacaggcgta agcgggcgga gccgaccgag tcgataccgg attcaaacgg
    16021 cacgtttttc gacctcgcgc gtgcgcgacc gcctaaaaac caaccgccta ccagcatcag
    16081 gcaacacagg ccaatggcaa cgataagaaa gatagcgaat gcccagtgat gagcgatgac
    16141 ttcagtggat gttgacatac tcattgctta ctcatcaaaa gtagcgccag attcactgct
    16201 cttcacggca gatggacgcc acatcgattc atggggagga ataaaaaaaa ccttacaatc
    16261 actgtagaaa ttcttttata cagctaattg atgtggtctt ttactccttt ctataacctt
    16321 ttgtcaactt taacaaaagt ttcttcacat tagtttacat aatatcaaca ccattagcat
    16381 ttaatgccct ttcacgccag atccttgacg actccaggat aattagatgt tgttgaatcg
    16441 tgtccgttgt gaagcaatgg aaaaaatacg ggtctatttt gacaggaatt tgtgtcgatt
    16501 cctcccccca aaagagagta ttttcttgat ctgtgacacg cttttgtcat tccataacaa
    16561 aaacgcagca acaaatttac gtatttttaa catcattgta gcaggtgatt tttttcaggc
    16621 gattatttgt gcgttcggga cgtgaatctc tggtgattga aaaatgaaca gttttgtacg
    16681 ttctgcacta tgcgaggaag gatttttact aaaaaaaagc cgctggggtt taaaacaccc
    16741 ccagcggctc gtttttacac tattgtctca ggaattatct atcgtccgtc gattactcga
    16801 tatccctttc aatcaacaat gaatcatccc cttccggagc agacattggg ctgtattgcc
    16861 acggattatg gtagctctcc atcgcctgat agatcacctg ggccagctca ttattactgg
    16921 acgggtcata gcacagcaaa tattcggtat caggtagcgg cggtaagcca tctactccac
    16981 tcagcacacg caggtccggg ctcatcattt caatcggcct tgccgtcacg ccaagaccgg
    17041 ctttcactgc cgcacgaacg gccggaagcg tcgaggcgac ataagccagt cgccatggaa
    17101 tatctgcttt attaagcgtc gccagcacca tatcgcgaaa cggacttgga tcatccagca
    17161 atacaagagg gatcggctcg cctttttgca atacgtattc cgctgcgcag taccagtgtg
    17221 ttggcgaggt acgcaggttc aatgccttaa acgctgaagg acgatgggtg gttaccatca
    17281 aatccacttc ctgggattcc agcatttctg ccatataggc attacgttta acgcggacat
    17341 ccagcgctaa tttcggataa accgaactca cgcgatttaa caggaaaggt aagatcgtat
    17401 cggcagattc atctgaagca ccgatagtta acacgccctg aagattactg aacattaatg
    17461 atgagcaggc ctcatcatta aaacgcagta ttttcctggc gtaaccaaga agttgaatgc
    17521 catgttcagt taacagtttg ttgcgaccgt gacgagcgaa cagttctttc ccaacgagtt
    17581 gttccagacg ctgcatttgc tgacttacgg cggactgagt acgacacaca gcggcagctg
    17641 cggcagcaaa agtgttcaga tcggcaacag caacaaatgt tctcagcaga tcgaggtcga
    17701 ggttaattat cggacgattt gcacttatca tatattatca cttactggcg gctcatactg
    17761 agctggttaa tgctgtgcac acacaaacaa gcaattccat ttgtaatgtg cctccctggc
    17821 agtttcatcc ggaacccgga cgaaagtaaa aatgcatatg agttgcacta aaaaagcgac
    17881 tcacattgtt ccgttataat gcctgaagta gatcacagaa tatatcttca gggatcgcat
    17941 atctattaag ttactcactc ttttctattt atgacatgcg cgtgtttgta taaatgtaaa
    18001 tgtgagtcct tgttccactc tcgtgcagca tcgctggtca tacgcgaaca cgtacaaaca
    18061 gcaatggtga ggcggcatca agagcaggga tccgtcattt atgcgagcat tttaccccaa
    18121 aaacctttta tttataaggg tcattgcgaa ttatctgatg caaagttatg ttatgttagg
    18181 caaagtaatc ttctattatt aataagcaca tcaaaacctt tttgaatatt aaataataat
    18241 taattagcat catcctcatt cattaattcc gcttaacaat agtttcacac cttgccccca
    18301 ttgcgacacc tcggaaatca tcacgctgtt aaactctccg taatgcggaa atatcatcat
    18361 cgcgtttcat tagtgaattc ttaaatgagg cattttcaca caattatctt acagataaaa
    18421 aaaccagact tgcaattaag aatcagaaca attcactata taacattgca tgcaaagcat
    18481 atacacctca ttattttgtc attattaagt tattaacagc acaatcgagc cttcccctct
    18541 ggcaaaatct tattctgcag accttcaaaa caccgtcctg ggggagtaca ttgttctaag
    18601 ctgacttcca cggcagggag tggcgataac agcaaaaaag gtcaagattc atgtccccca
    18661 ttgaaaaatc cagcaaatta gagaatgtct gttatgacat ccgtggtccg gtgctgaaag
    18721 aagcaaaacg cctggaagaa gaaggtaaca aggtactgaa actgaacatc ggcaacccag
    18781 ccccattcgg ttttgacgcg ccagatgaaa tcctcgttga cgtgatacgc aacctgccta
    18841 ccgctcaagg gtattgcgat tccaaagggc tttactccgc gcgtaaagcc atcatgcagc
    18901 actaccaggc tcgtggcatg cgtgatgtta ccgtggaaga tatttacatc ggcaatggtg
    18961 tatcggagct tatcgttcag gcaatgcagg cattgctgaa cagcggggac gaaatgttgg
    19021 ttcctgcacc agattaccca ctctggaccg cggcggtttc gctttccagc ggtaaagcgg
    19081 tgcattatct ttgcgatgaa tcctctgact ggttcccgga cctcgatgat attcgcgcta
    19141 aaattacgcc tcgtacgcgt gggatcgtta ttatcaaccc aaataaccca accggcgcgg
    19201 tatattccaa agagctttta atggagattg tggagattgc acgtcagcat aatctcatta
    19261 tcttcgccga tgaaatttat gacaaaattc tctacgacga tgctgagcat cactcaattg
    19321 cgccactggc acctgacctg ctgaccatta cctttaacgg actgtcgaaa acgtaccgcg
    19381 ttgcaggctt ccgtcagggg tggatggtgt tgaacgggcc gaaaaaacac gccaaaggct
    19441 acatcgaagg tctggaaatg ctggcttcaa tgcgcctgtg tgctaacgtt cctgcgcaac
    19501 acgccattca gaccgcgctg ggtggttatc agagcatcag tgaatttatt acccctggcg
    19561 gtcgtcttta tgagcagcgt aaccgcgcgt gggaactgat caacgatatt ccgggcgttt
    19621 cctgcgtgaa acctcgtggt gcgctgtata tgttcccgaa aatcgacgcc aaacgcttta
    19681 acattcacga cgatcagaaa atggtgctgg atttcctgtt gcaggaaaaa gttctgttgg
    19741 tgcaagggac ggcattcaac tggccgtggc cggatcactt ccgcattgtc acgctaccgc
    19801 gtgtcgatga tatcgagctg tctttgagca agttcgcgcg tttcctttct ggttatcatc
    19861 agctgtaatc ttaatttcac tgccggagat tgcatccggc agcgttatcc cgccacaatg
    19921 acctgatgat gtcatcatac gtaaggtcac tatgaaacag agccattttt ttgcccatct
    19981 ctcccgcctg aaactcatta accgctggcc gctaatgcgc aacgtgcgga cggaaaatgt
    20041 gtccgaacac agtttgcagg tagcgatggt cgcccatgcg ctggcagcta tcaaaaatcg
    20101 aaaatttggc ggtaatgtca acgccgaacg tatcgcttta ctggcgatgt accacgatgc
    20161 ctcagaagtg ctcaccggcg atctccctac tccggtgaaa tacttcaatt cgcaaatcgc
    20221 tcaggagtac aaggctattg aaaaaatcgc tcagcaaaaa ctggtcgata tggttccgga
    20281 agagctgcgg gatatctttg cgccgttaat tgacgagcat gcatatagcg atgaagaaaa
    20341 atcgctggtg aaacaggcag atgcactgtg tgcatatctg aaatgtctgg aagaactcgc
    20401 ggccggaaat aatgaattct tgctggcaaa aacgcgactg gaagcgacgc ttgaagcgcg
    20461 tcgcagccag gagatggact acttcatgga agtatttgtt cccagcttcc atctttcgct
    20521 cgatgagatt agccaggatt caccgctgta agcagccgga gtctgcgtcg catcaggcaa
    20581 taagcgccgg atgcgacatc aggctcttgt caaaacggaa acagcatcgg gatcatcacc
    20641 acacaaaccg ccatcacgat aatggtgaac ggtaccccca acttcacaaa gtcactgaag
    20701 ctgtaatttc ccggacctaa aaccagtgtg ttaacaggtg aagaaaccgg tgtcataaag
    20761 gcggcggatg ctgccatcgc cacgaccatc gcgaatggat aaggcgacac ccccatcgtt
    20821 ttggcagcag ccagcgcaat cggagccatc aacaccgccg tcgcggtatt agagataaat
    20881 agcccaataa ccgccgacaa gacaaacaaa caccccagca tcatatgtgg cccgtaacca
    20941 ccgccaatgt ccatcagccc tttcaccgcc agcgcgacac ctcccgtttt ctgtaatgcc
    21001 acagcaaacg gcatcatccc aacgatcaaa ataatgctcg gccagtgaat ggatttatag
    21061 gcgctttcag catctataca gcggaatttc cccatcagca ggcaggcgat gatagcggca
    21121 acaggattag gaatttcatc tgtcagcatt aacgccacca ttagcaccag acagaaaatg
    21181 gcatggggtg cctggctgtg cgcgggcgat gcttcactca cctcttccgg taagttcagc
    21241 gctacgaagt cgcggccctg tttggccagc ataccgatca gtttccagtt acccacaacc
    21301 aggatgatat cgcccagcag cagaggctca tccgccagcg aaccttccag cgccacgcca
    21361 ttgcgcttta gccccaccac attcagtccg tagcgggtac gaaaaccaat ttcgcgcacc
    21421 gatttaccaa tcagttctga ctcaggaatt aatgaaatct ctgccatgcc cacatcaagg
    21481 gcctggtcag aaaaatactc gccgcgcagt accatcggct ccagcaattg ctcactacaa
    21541 aattgccgga gatcgacatc agccgcagac atatcaataa gcaaaacgtc acgcgcgcga
    21601 aattcagaaa ccccattaac gttcacgata acgcgacgaa aacgccgcca gcgttcaaca
    21661 ccgatgacgt tagcgccata acgctcacgt aatttgagat catccagccg ttgaccaatc
    21721 attggcgatc cggggcgaat agccagacgt cgcgcacgcc cggtcagtcg atattcacgg
    21781 ataagatcgc gaaaggttcg acgcgtccag ccttcgcgct gcggggtctg ggtatcccct
    21841 ttcagcatga aacgcatcac taacatatac aaaataccca gcaccagcac gaccaggcca
    21901 ataggtgtta cgctaaagaa actgaagccg tgatagcctt cacgcagcaa ttcactgttg
    21961 actaccaggt tcggcggcgt cgccaccagc gtcatcatgc cgcttatcag cccggcaaaa
    22021 ctcaacggca tcatcagacg tgacggcgaa gtttgcatac gcatggcaac gcttaacacc
    22081 acgggaataa agatagcgac aacgccggtt gaactcataa acgcgccaag ccccgcgacg
    22141 gtcagcatca acaaaaccaa cattttgatt tcactgttgc ccgcaacttt gaccagccat
    22201 gttcccatta cggtggcaac accggtacgg accaaaccat cgccaataat aaacaaggcg
    22261 gcaatcagga caacgttagg atcagaaaag ccggaaaata cttcggggac tgtcagcgtt
    22321 ccgcttaatg caaacgcgac aataacaaac aaagcgaccg catccatacg cactctgccc
    22381 gtcgcaaaca agacaatggc aacggccagt aatgaaagaa cccaaatcaa ttcaccgttc
    22441 acaacttatc cttgttaatt gagggagatg tcttgattct gccataaaaa agccccgacg
    22501 agacggggct aaatcatgat caggtgtttc actgaataat aacatcgcca tttggctgtt
    22561 tggtcacagt aatttgctcc agactgtgga ggaccaaatc gacctcattc aggcgcgggg
    22621 tatctgccgg agcgttaacc gcaatgacat gacaacccgc cgccaggcca gaaagcacgc
    22681 cagcgggagc atcttccacc accacacact cctgcggcgc aagccccagc agctgcgcgc
    22741 ctaacagata cgcatcaggt tctggttttc cgcgcttcac tcgctcagcg gttacaaaca
    22801 cctctggtgc gggaagccca gctattttat ggcgcgctcg cgctaccggc atggagccag
    22861 aagtcacaat ggcccacgga atacctgctt tattcaaatg actgagtaag gcgattgccc
    22921 ccggaagcgg gtaatacctt cggtttccgt ggcctcgatg tgctccagac gcgtaaactc
    22981 ggcggcaata tcagcctcgg atttgcccgc cataaaatgg cgcagagagg tgatcgcctg
    23041 tttaccgtga atgaaagcca gcacctcttc cggcgctaac ccatgacgtc tggcccagtt
    23101 gctccacgcc cgttctaccg caggcaggga atccaccagc gttccatcaa gatcaaacag
    23161 aaaacctttg caccgcacgc gggcctcctc aggcattgat aatttgatta atttcgttgg
    23221 cgctcaaatg gtactgacgc gggcaggcat gccacacatt aagcatgcgc tgatattttt
    23281 cccacattgg cgtctgggcg ttaaaaccgt gagttccggc gtcaaaatgg gtatagcgcc
    23341 cttccacatt aaccataaag cggacataac cgaggtaacg tgcttcagtg gcggcgtcaa
    23401 agccgaggaa ggtgacacga cgttcatcga tggattgctg gtcctgcaaa ttagaccagg
    23461 aaacatgcaa cgcatgatac atctccataa tgtcgatgat agtgcggcag gtttcttctt
    23521 tcagctcgcc aaactcgcga tccaattcac gcatctgtaa tccgtaacca cgctcaataa
    23581 ttgtttgcag gcgacggtaa cgttcagcat ttgccggatc gagcatagtc atcatcttgt
    23641 actgattaga caaaataaga cgttgcgcgt tggtcatttc cattgttgac tcctgtatca
    23701 ctctactacg gtgaaaaaaa agaaggctga gtatgccttc ttttatatgc gtaatcaggg
    23761 gtcaattaca aatcatcaag gaaagtttta tccagttgtt tgaaggcgcg cttaagcgtg
    23821 tcagctaatg cctggtaatc aggcttgcct tcaacgggtg ccaacacctg tccagactcc
    23881 tgcaatttac cgcgaacttc ataaaaccag ttaaggattg cagggggtaa tggcgttaca
    23941 gaacgcttgc ccagccacca caatccctgc atgggtaaac ttaaggcgaa cagtgcagtg
    24001 gcaactgccg gcccaagctg accgcccagg gcaatctgcc agcagagagt aaatacggcg
    24061 atcggcggca taaaacggat cgcataacgc gtcatcttga taacgcgatt ttcgacaaag
    24121 accggggcaa ggcgtttttc cagtggccac gtctttgagt aatgctgtcc gcggcgaaac
    24181 aagctaaaaa aattaacaga acgattatcc ggcgttgaca tgcttcacct caacttcaca
    24241 tataaagatt caaaaatttg tgcaaattca caactcagcg ggacaacgtt caaaacattt
    24301 tgtcttccat acccactatc aggtatcctt tagcagcctg aaggcctaag tagtacatat
    24361 tcattgagtc gtcaaattca tatacattat gccattggct gaaaattacg caaaatggca
    24421 tagactcaag atatttcttc catcatgcaa aaaaaatttg cagtgcatga tgttaatcat
    24481 aaatgtcggt gtcatcatgc gctacgctct atggctccct gacgtttttt tagccacgta
    24541 tcaattatag gtacttccat gtcgagtaag ttagtactgg ttctgaactg cggtagttct
    24601 tcactgaaat ttgccatcat cgatgcagta aatggtgaag agtacctttc tggtttagcc
    24661 gaatgtttcc acctgcctga agcacgtatc aaatggaaaa tggacggcaa taaacaggaa
    24721 gcggctttag gtgcaggcgc cgctcacagc gaagcgctca actttatcgt taatactatt
    24781 ctggcacaaa aaccagaact gtctgcgcag ctgactgcta tcggtcaccg tatcgtacac
    24841 ggcggcgaaa agtataccag ctccgtagtg atcgatgagt ctgttattca gggtatcaaa
    24901 gatgcagctt cttttgcacc gctgcacaac ccggctcacc tgatcggtat cgaagaagct
    24961 ctgaaatctt tcccacagct gaaagacaaa aacgttgctg tatttgacac cgcgttccac
    25021 cagactatgc cggaagagtc ttacctctac gccctgcctt acaacctgta caaagagcac
    25081 ggcatccgtc gttacggcgc gcacggcacc agccacttct atgtaaccca ggaagcggca
    25141 aaaatgctga acaaaccggt agaagaactg aacatcatca cctgccacct gggcaacggt
    25201 ggttccgttt ctgctatccg caacggtaaa tgcgttgaca cctctatggg cctgaccccg
    25261 ctggaaggtc tggtcatggg tacccgttct ggtgatatcg atccggcgat catcttccac
    25321 ctgcacgaca ccctgggcat gagcgttgac gcaatcaaca aactgctgac caaagagtct
    25381 ggcctgctgg gtctgaccga agtgaccagc gactgccgct atgttgaaga caactacgcg
    25441 acgaaagaag acgcgaagcg cgcaatggac gtttactgcc accgcctggc gaaatacatc
    25501 ggtgcctaca ctgcgctgat ggatggtcgt ctggacgctg ttgtattcac tggtggtatc
    25561 ggtgaaaatg ccgcgatggt tcgtgaactg tctctgggca aactgggcgt gctgggcttt
    25621 gaagttgatc atgaacgcaa cctggctgca cgtttcggca aatctggttt catcaacaaa
    25681 gaaggtaccc gtcctgcggt ggttatccca accaacgaag aactggttat cgcgcaagac
    25741 gcgagccgcc tgactgcctg atttcacacc gccagctcag ctggcggtgc tgttttgtaa
    25801 cccgccaaat cggcggtaac gaaagaggat aaaccgtgtc ccgtattatt atgctgatcc
    25861 ctaccggaac cagcgtcggt ctgaccagcg tcagccttgg cgtgatccgt gcaatggaac
    25921 gcaaaggcgt tcgtctgagc gttttcaaac ctatcgctca gccgcgtacc ggtggcgatg
    25981 cgcccgatca gactacgact atcgtgcgtg cgaactcttc caccacgacg gccgctgaac
    26041 cgctgaaaat gagctacgtt gaaggtctgc tttccagcaa tcagaaagat gtgctgatgg
    26101 aagagatcgt cgcaaactac cacgctaaca ccaaagacgc tgaagtcgtt ctggttgaag
    26161 gtctggtccc gacacgtaag caccagtttg cccagtctct gaactacgaa atcgctaaaa
    26221 cgctgaatgc ggaaatcgtc ttcgttatgt ctcagggcac tgacaccccg gaacagctga
    26281 aagagcgtat cgaactgacc cgcaacagct tcggcggtgc caaaaacacc aacatcaccg
    26341 gcgttatcgt taacaaactg aacgcaccgg ttgatgaaca gggtcgtact cgcccggatc
    26401 tgtccgagat tttcgacgac tcttccaaag ctaaagtaaa caatgttgat ccggcgaagc
    26461 tgcaagaatc cagccctctg ccggttctcg gcgctgtgcc gtggagcttt gacctgatcg
    26521 cgactcgtgc gatcgatatg gctcgccacc tgaatgcgac catcatcaac gaaggcgaca
    26581 tcaatactcg ccgcgttaaa tccgtcactt tctgcgcacg cagcattccg cacatgctgg
    26641 agcacttccg tgccggttct ctgctggtga cttccgcaga ccgtcctgac gtgctggtgg
    26701 ccgcttgcct ggcagccatg aacggcgtag aaatcggtgc cctgctgctg actggcggct
    26761 acgaaatgga cgcgcgcatt tctaaactgt gcgaacgtgc tttcgctacc ggcctgccgg
    26821 tatttatggt gaacaccaac acctggcaga cctctctgag cctgcagagc ttcaacctgg
    26881 aagttccggt tgacgatcac gaacgtatcg agaaagttca ggaatacgtt gctaactaca
    26941 tcaacgctga ctggatcgaa tctctgactg ccacttctga gcgcagccgt cgtctgtctc
    27001 cgcctgcgtt ccgttatcag ctgactgaac ttgcgcgcaa agcgggcaaa cgtatcgtac
    27061 tgccggaagg tgacgaaccg cgtaccgtta aagcagccgc tatctgtgct gaacgtggta
    27121 tcgcaacttg cgtactgctg ggtaatccgg cagagatcaa ccgtgttgca gcgtctcagg
    27181 gtgtagaact gggtgcaggg attgaaatcg ttgatccaga agtggttcgc gaaagctatg
    27241 ttggtcgtct ggtcgaactg cgtaagaaca aaggcatgac cgaaaccgtt gcccgcgaac
    27301 agctggaaga caacgtggtg ctcggtacgc tgatgctgga acaggatgaa gttgatggtc
    27361 tggtttccgg tgctgttcac actaccgcaa acaccatccg tccgccgctg cagctgatca
    27421 aaactgcacc gggcagctcc ctggtatctt ccgtgttctt catgctgctg ccggaacagg
    27481 tttacgttta cggtgactgt gcgatcaacc cggatccgac cgctgaacag ctggcagaaa
    27541 tcgcgattca gtccgctgat tccgctgcgg ccttcggtat cgaaccgcgc gttgctatgc
    27601 tctcctactc caccggtact tctggtgcag gtagcgacgt agaaaaagtt cgcgaagcaa
    27661 ctcgtctggc gcaggaaaaa cgtcctgacc tgatgatcga cggtccgctg cagtacgacg
    27721 ctgcggtaat ggctgacgtt gcgaaatcca aagcgccgaa ctctccggtt gcaggtcgcg
    27781 ctaccgtgtt tatcttcccg gatctgaaca ccggtaacac cacctacaaa gcggtacagc
    27841 gttctgccga cctgatctcc atcgggccga tgctgcaggg tatgcgcaag ccggttaacg
    27901 acctgtcccg tggcgcactg gttgacgata tcgtctacac catcgcgctg actgcgattc
    27961 agtctgcaca gcagcagtaa cctcgtcatc atccgcagct ttgcgctgcg gatatctgaa
    28021 ccggaaataa tcactatttc cggtttttta ttctcttaat ctgcattaat cctttctgat
    28081 tatcttgctt aactgcgctg catcaatgaa ttgcgccatt tcactttgca tacttaccac
    28141 tttgttttgt gcaagggaat atttgcgcta tgtccgcaat cactgaatcc aaaccaacaa
    28201 gaagatgggc aatgcccgat acgttggtga ttatcttttt tgttgctatt ttaaccagcc
    28261 ttgccacctg ggtagttccg gtggggatgt ttgacagtca ggaagtgcag tatcaggttg
    28321 atggtcaaac aaaaacacgc aaagtcgtag atccacactc atttcgcatt ctgactaacg
    28381 aagcaggcga acctgagtat caccgcgtac agctgttcac gacgggcgat gaacgcccgg
    28441 gcctgatgaa cttcccgttt gaaggattaa cctcaggatc gaaatacggg acagctgttg
    28501 gcatcatcat gtttatgctg gtgattggcg gcgcgtttgg cattgtgatg cgtacaggaa
    28561 ccattgataa cggtatcctg gcgcttattc gccatacccg cgggaatgaa attctcttta
    28621 ttcctgcgct gtttattctg ttttcacttg gcggcgcggt atttggtatg ggagaagagg
    28681 ccgtcgcctt tgccattatc atcgcaccgc taatggtccg gctgggctat gacagtatta
    28741 ccaccgtcct ggtgacctat attgccacgc aaatcggttt tgccagttcg tggatgaacc
    28801 cgttttgtgt ggtcgttgct caggggattg ccggcgttcc ggtgctttct ggctccgggt
    28861 tgcgcatcgt ggtgtgggtt atcgccacta tgattggcct gatctttacc atggtgtacg
    28921 cctcacgagt gaaaaagaat cctcttctgt cacgcgtgca tgagtccgac cgcttctttc
    28981 gtgaaaagca ggcggatgtt gaacaacgtc cgtttacctt tggtgactgg ctggtattga
    29041 ttgtcctgac cgccgtaatg gtctgggtga tttggggcgt gatcgttaat gcctggttta
    29101 ttccagaaat tgccagccag ttcttcacca tgggtctggt gattggcatc atcggcgttg
    29161 ttttccgcct taacggcatg acggttaata ccatggcttc atcctttacc gaaggggcgc
    29221 gaatgatgat cgcccctgcc ctgctggtgg gtttcgccaa agggattttg ctgctggtcg
    29281 gtaatggtga agcgggtgat gccagcgtgt taaataccat cctcaacagc attgccaatg
    29341 ccattagcgg tctggacaac gcggtcgcgg cctggtttat gttgctcttc caggcggtat
    29401 ttaatttctt cgtgacgtcc ggttctggtc aggcggcgtt aaccatgccg ttactggcac
    29461 cgcttggcga tctggtcggt gttaaccgtc aggttaccgt gctggcattc cagtttggtg
    29521 atggcttcag tcacatcatt tacccaacct cagcttcgtt aatggcaact ctcggtgtgt
    29581 gcagggtgga cttccgtaac tggctgaagg tgggcgcgac cctacttgga ctgctgttta
    29641 ttatgtccag cgtcgtagtg atcggcgctc agttgatggg ctaccactaa aaatgttaag
    29701 agccgcaatt gcggctcttt tttcattctg ccgtttcagt ctctacggct tcatttttgg
    29761 cattgcgctt catccacaac gccagcgctt tcagcgagtc tggagtgaac tcatcgcagc
    29821 gtgcggtgat ttcttccggc gtcagccagc aaacttcact gacttcatct tcctgtaggg
    29881 cgaagggacc gtgagagacg cagctgaaca acgcgcccca gacacggcaa tttttatctt
    29941 cgaaatagaa ctgcccgtgc tcggcaaagg ggacaccggc aatgcccaac tcttcttccg
    30001 cttcgcgacg cgcggattcc agcagttgct catcggcctg aaccacaccg cctgcggtcg
    30061 catctaacat gccgggtaaa aagtcttttg tctcggtacg acgctggacc agaattttgc
    30121 ccatgccatc atgcacgacg atgtaagttg cacgatgacg cagacactgt gcccgcattt
    30181 gttcccggct ggcttgtgca atgacttcgt tctcttcatt gacaatatcc acccattcag
    30241 tacttgccaa acgacgctgt tccattatca ggaaaccttc tttttctggc gctcttacgg
    30301 cgcattttga gttgtgggta aattacggat taatcgcgac ctgcgcaatg atactttgat
    30361 cattgagtgc gattacacta agtacgtcat tatccagcat gccataactc gccggattac
    30421 cgcctttcgg aatactcacc gagccggggt tgaagtggaa aatctccccc cgctgttctg
    30481 ccactggcag atgggtatga ccgtacacca gcacatcgtt ctggtttaaa gcaggtagat
    30541 tttccgggcc aaaaagatga ccatgcgtca aaaacagacg ttgtttttcc agtaatacct
    30601 gttgccacgg cgcggttatc gggaaatgca gcagcatttg atccacttcg ctgtcgcagt
    30661 tgccgcgcac agcgataacc ttatgtgcca cttcattaag ccgttcagcg actttggctg
    30721 gcgcgtaacc ctccggtaaa gcattacgcg ggccgtgatt caacacgtcg ccaaggatca
    30781 ccagccactg ggcaccgctt tgggcaaaca actccagaac acgttccgtc gccggtaacg
    30841 ccccatgaat gtccgatgca aacatcagtt tcatcactca ctcctcgtcg aaaaaacgta
    30901 agctatgata ctggattctg tcgttgctat cagccagatt gcttcgccga aagtgcaata
    30961 aaacgctgga cagacgctcg ctgccactgg aatgtcgcat aatccaccag gcgttctggc
    31021 acctcatcgc catgtagtac caggcggtta atcattagcg ccagatcagt atcagcaatg
    31081 caccattcac caaataaatt cggctgaccc agtgctaaca aatgttctgc catcgcgaac
    31141 agtttctctg cactggcttt tccctcggcc gttagtggcg cttttttcgc ccccgcaaag
    31201 acaacatccg tcggacgctc ttcgcggatg ggcatcagat cgctgcgcag ccaggcctga
    31261 atccgtcgtg cacgcgcacg attttctaaa tcaagcggat aaatacgttc ccaggtcggt
    31321 ggcgcaaatc gatcttccag atactccgca atggcagaag attcactcaa ctcaaaatca
    31381 tcgatttgta ataacggcac acggcgtgtc tgaccgtaac cttgccacgt cggcttcaaa
    31441 tgttcaccgc tgtcgaggtc gatggtcttg atatgaaacg acaggccttt ttcctgcaac
    31501 gccacccagg cggataacac ataaggggag aaaaagtggg catctgacca aagcgtgata
    31561 gcgggtttac tcattatgtc ctcggcaata aagggggctg gctatcaaac tatagagcct
    31621 ttttgtcact gtcacgtgac gaatactcac gtaagtcgcc gcgctttcta tacttgtggt
    31681 gttcagatga ttaaacaacc ggagctgcaa tgatcgatct ctatttcgcc ccgacaccca
    31741 atggtcacaa aattacgctg tttctcgaag aagcagagct tgattatcgc ttgattaagg
    31801 tagacctggg gaaaggcggt cagtttcgtc cg
//
LOCUS       DAKISA010000042        30368 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.042, whole genome shotgun sequence.
ACCESSION   DAKISA010000042 DAKISA010000000
VERSION     DAKISA010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30368
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.042"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            395..2674
                     /gene="ydeP"
                     /locus_tag="OVW45_002028"
     CDS             395..2674
                     /gene="ydeP"
                     /locus_tag="OVW45_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCV5526481.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPHS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2922..3119
                     /gene="safA"
                     /locus_tag="OVW45_002029"
     CDS             2922..3119
                     /gene="safA"
                     /locus_tag="OVW45_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCV5526482.1"
                     /translation="MYATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            3194..3955
                     /gene="ydeO"
                     /locus_tag="OVW45_002030"
     CDS             3194..3955
                     /gene="ydeO"
                     /locus_tag="OVW45_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCV5526483.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHMGMNTGNTMSALAI"
     gene            4356..6038
                     /locus_tag="OVW45_002031"
     CDS             4356..6038
                     /locus_tag="OVW45_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCV5526484.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGAPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            6090..7247
                     /locus_tag="OVW45_002032"
     CDS             6090..7247
                     /locus_tag="OVW45_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCV5526485.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(7213..7335)
                     /locus_tag="OVW45_002033"
     CDS             complement(7213..7335)
                     /locus_tag="OVW45_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5526486.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            7544..9229
                     /locus_tag="OVW45_002034"
     CDS             7544..9229
                     /locus_tag="OVW45_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HCV5526487.1"
                     /translation="MITIPITFYMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQTELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISTVL"
     gene            9267..11639
                     /locus_tag="OVW45_002035"
     CDS             9267..11639
                     /locus_tag="OVW45_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCV5526488.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVTGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMAEHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            11696..14479
                     /locus_tag="OVW45_002036"
     CDS             11696..14479
                     /locus_tag="OVW45_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCV5526489.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETLYQVSLPTTQKQNLQQVMAIFSEWSNAATFEILEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSLAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            14841..16241
                     /locus_tag="OVW45_002037"
     CDS             14841..16241
                     /locus_tag="OVW45_002037"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCV5526490.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICAGRPDEGIPAVCFKLKEGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
     gene            16397..17932
                     /gene="gadC"
                     /locus_tag="OVW45_002038"
     CDS             16397..17932
                     /gene="gadC"
                     /locus_tag="OVW45_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCV5526491.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            18063..19382
                     /locus_tag="OVW45_002039"
     CDS             18063..19382
                     /locus_tag="OVW45_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCV5526492.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            19754..21136
                     /gene="dosC"
                     /locus_tag="OVW45_002040"
     CDS             19754..21136
                     /gene="dosC"
                     /locus_tag="OVW45_002040"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCV5526493.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNAHSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            21161..23560
                     /gene="dosP"
                     /locus_tag="OVW45_002041"
                     /gene_synonym="pdeO"
     CDS             21161..23560
                     /gene="dosP"
                     /locus_tag="OVW45_002041"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HCV5526494.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            23819..24400
                     /gene="ddpX"
                     /locus_tag="OVW45_002042"
     CDS             23819..24400
                     /gene="ddpX"
                     /locus_tag="OVW45_002042"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HCV5526495.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            24414..25964
                     /locus_tag="OVW45_002043"
     CDS             24414..25964
                     /locus_tag="OVW45_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCV5526496.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDTPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            25966..26988
                     /locus_tag="OVW45_002044"
     CDS             25966..26988
                     /locus_tag="OVW45_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCV5526497.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYFSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            26994..27890
                     /locus_tag="OVW45_002045"
     CDS             26994..27890
                     /locus_tag="OVW45_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCV5526498.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            27887..28873
                     /locus_tag="OVW45_002046"
     CDS             27887..28873
                     /locus_tag="OVW45_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCV5526499.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGYVHVLNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAITLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISI"
     gene            28902..29798
                     /locus_tag="OVW45_002047"
     CDS             28902..29798
                     /locus_tag="OVW45_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCV5526500.1"
                     /translation="MNFPARKSWLGKTTEHVHAINGIDLQIRRGETLGIVGESGCGKS
                     TLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLSVWRIITEPLWIAKRSS
                     EQQRRALAKELAVQVGIRPEYLDRLPHAFSGGQRQRIAIAIARALSSQPDVIVLDEPT
                     SALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYLGQIVELGDAQ
                     QVLTAPAHPYTRLLLDSLPAIDKPLEEEWALCKTDLPGNRTLPQGCFFYERCPLATHG
                     CEVRQSLAIREDGRELRCWRAL"
     gene            complement(29854..30285)
                     /gene="osmC"
                     /locus_tag="OVW45_002048"
     CDS             complement(29854..30285)
                     /gene="osmC"
                     /locus_tag="OVW45_002048"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCV5526501.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTQSGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDADFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         8137 a         7116 c         7195 g         7920 t
ORIGIN      
        1 aaacaaggtt tcagccggaa ccgtccagtc agtcattggt gtcactttta tctatgaata
       61 attgtgattt tgaccttagt ctatgcgctc cattcacgaa aattgcgcag ataattattt
      121 tgtgaaggct attatcctac acctgtaaga aaatccgcgc acaattgaag ccagagtctt
      181 attttttata ttgaaaaata tgttaattta aacactatga gagatgatgc ttaacttatc
      241 gtatttaatc tattgtttaa cgataataga ttaaaaatag aaagagagta gtcccctact
      301 cttatatatc catgttggcg ataatccctt tgtatgtact gtgcatcatc gctattacaa
      361 atcctaataa ttcatttcca cacaggataa gtagatgaag aaaaaaattg aatcctacca
      421 gggtgctgca ggtggttggg gtgctgttaa atccgtagcg aatgcagtac gtaagcagat
      481 ggatatacgc catgatgtta ttgccatgtt tgacatgaat aagccagagg gctttgactg
      541 tccgggttgt gcatggccag atcctaagca cagtgcgtca ttcgacattt gtgaaaacgg
      601 cgcaaaagca atcgcctggg aagtcacgga taagcaggta aatgcctctt tctttgctga
      661 gaatacggtt caatcattac ttacctgggg agatcacgag cttgaggctg cggggcgtct
      721 cactcagcct ttgaaatatg atgccgtcag cgactgttac aagccattaa gctggcaaca
      781 agctttcgac gaaattggcg cacgccttca aagctatagt gatcccaatc aggttgaatt
      841 ctatacttcg ggccgcactt ccaatgaagc tgcctttctt tatcagcttt ttgcccgtga
      901 atacgggagc aataactttc ccgactgctc caacatgtgc catgaaccga caagcgtggg
      961 tttggcagcg agtatcggtg taggtaaagg gaccgtgttg ctggaagact ttgagaagtg
     1021 cgatttagtc atttgcattg ggcataaccc tggtacaaac caccctcgca tgctgacttc
     1081 gttgcgcgct ttagtgaaac ggggagcgaa aatgatcgcc atcaatcctc tacaggaacg
     1141 tggcctggag cgatttaccg caccgcaaaa cccgtttgaa atgctgacga actctgagac
     1201 tcagttggcc agtgcctact acaacgtgcg cattggtggc gatatggcgt tgctcaaggg
     1261 gatgatgcgc ctgttaattg agcgcgatga tgctgcaagt gccgcaggtc ggccctcatt
     1321 gcttgatgac gaatttattc aaacgcatac cgtcggcttt gacgagctac gccgtgacgt
     1381 tctcaattcc gagtggaaag atatcgaacg tatttctgga ctaagtcaga cacaaatcgc
     1441 cgaactggct gacgcatatg ccgctgccga tcgcaccatt atctgttacg gaatggggat
     1501 cactcagcac gaacatggta cccagaatgt acagcaactg gtcaatctgc tgttgatgaa
     1561 aggtaacatt ggcaagcctg gtgcgggtat ctgcccacta cgaggtcact ctaatgtaca
     1621 gggcgaccga accgtcggta tcaccgagaa accgtctgca gagtttctgg ctcgcctggg
     1681 tgagcgctat ggcttcaccc cacctcatgc acctggacat gctgcaattg ccagcatgca
     1741 agcaatatgc acggggcagg ctcgagcatt gatctgcatg gggggcaact ttgcactggc
     1801 aatgccagat cgggaagcga gcgctgtacc gttaacgcaa ttagatttgg cggtacacgt
     1861 agccactaag cttaaccgct ctcatctgct gaccgcacgg catagctata ttctgccggt
     1921 tctggggcgt agcgagattg acatgcaaaa aagcggtgcg caggcggtaa ccgttgagga
     1981 ttcaatgtcg atgattcatg cctcgcgtgg cgtgttaaaa cccgccggtg taatgctgaa
     2041 atcagagtgt gcagtggtcg cgggaatcgc gcaggcagca ctaccccaca gcgtggtagc
     2101 ctgggagtat ctggtggaag attatgatcg cattcgcaat gacattgaag ctgtgttacc
     2161 agagttcgcc gactataacc agcgcatccg acatcctgga ggttttcacc tgataaatgc
     2221 agctgctgaa aggcgctgga tgacgccgtc aggtaaggct aatttcatta ccagcaaagg
     2281 gctgttagaa gatccctctt cagcgtttaa cagtaagctg gtcatggcga cagtacgtag
     2341 ccacgatcag tacaacacga cgatttatgg tatggatgat cgctatcgag gggtattcgg
     2401 tcaacgagat gtggtcttta tgagtgctaa acaagctaaa atttgccgtg taaaaaacgg
     2461 cgaaagagtt aatcttattg cgcttacgcc agacggtaag cgcagctcac gccgcatgga
     2521 tagattaaaa gtggtcattt accctatggc tgaccgctca ctggtgacct attttccaga
     2581 atcgaatcac atgctaactc ttgataacca cgatccatta agtggcattc ctggttataa
     2641 aagtattccg gttgaattag aaccatcaaa ttaattcgcc tcctcatttc ttctgctgtc
     2701 atccgcacag cagaagaatt tctcattgac tattatcgcg caatttgctc acattgatta
     2761 aattaaacca cacactataa gatataaacc tctgcctaca gctgtaagaa actccgctca
     2821 gtactgaagt gccagtccta tttcctcttt tctccagcct gttatattaa gcatactgat
     2881 taacgatttt taacgttatc cgctaaataa acatatttga aatgtatgcg accacagtga
     2941 aaaacaaaat cacgcaaaga gacaactata aagaaatcat gtctgtaatt gtggttgtct
     3001 tattactgac ccttacgttg atagccattt tttcggcaat tgatcagctg ggtatttcag
     3061 aaatgggtcg cattgcaaga gatcttacac atttcattat caatagtttg caagactgaa
     3121 aataaacagc aaattataaa tatgaaatgt taaaaaagta tcgataaaaa ctttattgtt
     3181 ttaaggagat aaaatgtcgc tcgtttgttc tgttatattt attcatcatg ccttcaacgc
     3241 taacatttta gataaagatt acgccttctc tgacggcgag atcctgatgg tagataacgc
     3301 tgttcgtacg cattttgaac cttatgagcg gcattttaaa gagatcggat ttactgaaaa
     3361 taccattaaa aaatatctac aatgcactaa catccagaca gtgacggtgc ctgttcctgc
     3421 gaagttttta cgtgcttcaa atgtaccgac tggattgctt aatgaaatga ttgcttatct
     3481 taactcggaa gaacgcaatc atcataattt ttcagaactt ttgctttttt cttgcctgtc
     3541 tatttttgcc gcatgcaaag gtttcattac actattaact aacggtgtgc tatccgtttc
     3601 cggaaaagtg agaaatattg tcaacatgaa gctggcgcac ccatggaagc tgaaagatat
     3661 ttgtgactgc ctgtacatca gtgaaagcct gttgaagaaa aaactaaagc aagagcaaac
     3721 gacattctca cagattctat tagatgccag aatgcagcac gcaaaaaatt tgatacgcgt
     3781 agaaggttca gtcaataaaa ttgccgaaca atgtggttat gccagtacat cttattttat
     3841 ttatgcgttc cgcaaacatt tcggcaacag tccgaagaga gtttctaagg agtaccgttg
     3901 tcaaagtcac atgggtatga atacgggcaa cacgatgagt gctttagcta tttgattatt
     3961 tgctaacgag tagtcaacca cgcacgctgc gtaagaatta aatggggcag ccattccctg
     4021 ccccgcgttg tttttaggcg atatatttat tgaaataaat aagtgacatc catcacatat
     4081 ttatgcactt gcataacctg ttgcagatta tttatgatct caattcggca ttttgtcagt
     4141 aaaatgcaat aatttattaa atatcaataa attagttgtt tatcggcgag aaattactta
     4201 atagaacaga aagtaatgtc aacgctttat ggactgttat ttcccttttt ttagctaaat
     4261 ctgctatctc tttatgtgac taacttcact tacatccact tatttctctt cgtaaaatta
     4321 cttcggaatt aagtacaata agaagaggaa catttatgaa gtctgcatta aagaaaagtg
     4381 tcgtaagtac ctcgatatct ttgatactgg catctggtat ggctgcattt gctgctcatg
     4441 cggcagatga tgtaaagctg aaagcaacca aaacaaacgt tgctttctca gactttacgc
     4501 cgactgaata cagtaccaaa ggaaagccaa atattatcgt actaaccatg gatgatcttg
     4561 gttatggaca acttcctttt gataagggat cttttgaccc aaaaacaatg gaaaatcgtg
     4621 aagttgtcga tacctacaaa atagggatag ataaagccat tgaagctgca caaaaatcaa
     4681 cgccgacgct cctttcatta atggatgaag gcgtacgttt tactaacggc tatgtggcac
     4741 acggtgtttc cggcccctcc cgtgccgcaa taatgaccgg tcgagctccc gcccgctttg
     4801 gtgtctattc caataccgat gctcaggatg gtattccgct aacagaaact ttcttgcctg
     4861 aattattcca gaatcatggt tattacactg cagcagtagg taaatggcac ttgtcaaaaa
     4921 tcagtaatgt gccggtaccg gaagataaac aaacgcgtga ctatcatgac aacttcacca
     4981 cattttctgc ggaagaatgg caacctcaaa accgtggctt tgattacttt atgggattcc
     5041 acgctgcagg aacggcatat tacaactccc cttcactgtt caaaaatcgt gaacgtgtcc
     5101 ccgcaaaagg ttatatcagc gatcagttaa ccgatgaggc aattggcgtt gttgatcgtg
     5161 ccaaaacact tgaccagcct tttatgcttt acctggctta taatgctccg cacctaccaa
     5221 atgataatcc tgcaccggat caatatcaga agcaatttaa taccggtagt caaacagcag
     5281 ataactacta cgcttccgtt tattctgttg atcagggtgt aaaacgcatt ctcgaacaac
     5341 tgaagaaaaa cggacagtat gacaatacaa ttattctctt tacctccgat aatggtgcgg
     5401 ttatcgatgg tcctctgccg ctgaacgggg cgcaaaaagg ctataagagt cagacctatc
     5461 ctggcggtac tcacacccca atgtttatgt ggtggaaagg aaaacttcaa cccggtaatt
     5521 atgacaagct gatttccgca atggatttct acccaacagc tcttgatgca gccgatatca
     5581 gcattccaaa agaccttaag ctggatggtg tttccttgct gccctggttg caagataaga
     5641 aacaaggcgc gccacataaa aatctgacct ggataacctc ttattctcac tggtttgatg
     5701 aggaaaatat tccattctgg gataattacc acaaatttgt tcgccatcag tcagacgatt
     5761 acccgcataa ccccaacact gaggacttaa gccaattctc ttatacggtg agaaataacg
     5821 attattcgct tgtctataca gtagaaaaca atcagttagg tctctacaaa ctgacggatc
     5881 tacagcaaaa agataacctt gccgccgcca atccgcaggt cgttaaagag atgcaaggcg
     5941 tggtaagaga gtttatcgac agcagccagc caccgcttag cgaggtaaat caggagaagt
     6001 ttaacaatat caagaaagca ctaagcgaag cgaaataact aaaccttcat gcggcggatt
     6061 tttccgccgc cttattgagc gagatagcga tgcacgttac agccaagccc tccagttttc
     6121 aatgtaatct caaatgtgat tactgttttt accttgaaaa agagtcgcag tttactcatg
     6181 aaaaatggat ggatgacagc actctgaaag agttcatcaa acaatatatc gaagcgtctg
     6241 gcaatcaggt ctattttacc tggcaaggcg gtgaacccac tctggctggc ctggattttt
     6301 tccgtaaagt tattcactat caacaacgct atgcaggcca aaaacgtatt tttaatgcat
     6361 tacaaacgaa tggcatttta ttgaataatg aatggtgtgc ctttctcaaa gaacatgaat
     6421 ttctggttgg tatctcgatc gatggccccc aggagttaca tgaccgttac agacgcagta
     6481 attcaggtaa cggtactttt gcaaaagtga tagcagccat cgagcgtctg aaatcatatc
     6541 aagtagagtt taatacgtta accgtcatta ataacgttaa tgtccattac cctcttgagg
     6601 tttatcattt tttaaaatct atcggcagta aacatatgca atttatcgaa ttgctagaaa
     6661 ccgggacgcc gaatattgat ttcagtggtc atagtgagaa cacattccgt atcattgatt
     6721 tttctgtgcc tcccacggct tatggcaagt ttatgtcaac catttttatg caatgggtta
     6781 aaaacgatgt gggtgaaatt ttcattcgtc agtttgaaag ctttgtcagc cgttttttgg
     6841 ggaatgggca tacaagttgt attttccagg agtcctgcaa ggataatctg gttgttgaaa
     6901 gtaatggaga catttacgaa tgcgaccatt ttgtctatcc acagtacaaa attggaaaca
     6961 ttaataaatc tgaactcaaa acgatgaaca gtgtacaact gacagcgcaa aaaaaacgga
     7021 tttcagcgaa atgtcagcaa tgtgcatata aacctatctg caatggcggt tgtcctaagc
     7081 atcgtattac taaagtaaac aatgagactg tttcttattt ttgcgaaggt tataaaatcc
     7141 ttttttcaac catggtacct tatatgaacg ccatggttga gttagctaag aacagagtac
     7201 cgctttacca cattatggat gttgcaaaac aaatggagaa taattaataa cgctctcttt
     7261 taactccatt cttagcctcc ttcttatgac cttaacaagg cacccgacat aaattcctgt
     7321 cgggtgtttc gtcatcatca tcttcccttg cgaaaatcct ggcttacgtg gtatgcgtca
     7381 gggcattagc aatacccttg attatcaata gcataaccat aaccataacc actgaaaatt
     7441 aaaaatttct atatatttca tattgttaaa tgatacttaa ataaatttca tcagtaaatc
     7501 attgccatag ataatcattg gatggcacaa tcaattttcg ctaatgataa ccattcccat
     7561 tacgttttat atgttgatag caaagtatct ctgtctgctg aagccctttt ggctgcgtaa
     7621 gaataataaa acttcagtgt tgttaatcat tattatcctc gcaatgatcc ttggcgtggt
     7681 taaaattcag gtctggttaa acgactggaa taatgatttt ttcaatgcgc taagccagaa
     7741 agaaaccgat aagctttggc agcttgttct ttggttccct gccctgctgg ggatctttgt
     7801 attaatatcc gtaaacaaaa catggttaat taaactactg actattcgct ggcgcgaatg
     7861 gttaacagat tactacctta atcgctggtt tgcagataaa aactactact tcacgcagat
     7921 ctacggcgag cataaaaata cagataaccc tgaccaacgt atcgctgaag acattctctt
     7981 actgatcagc aaaacgctca gtctttcatt tggcttcatc cagtcactta gcatgctgat
     8041 cacctttacc gttattcttt ggcaaagcgc gggtacgctc tcattcactg ttggtggaac
     8101 agaatggaat atccaggggt atatggtcta taccgtcgtg ctcattgtga tcggtggaac
     8161 tttatttacc cataaggtgg gtaaacgtat tcgtccgctt aatgtggaaa aacaacgtag
     8221 cgaagcgaca tttcgaacta atcttgtgca gcataataag cagacagaac tgattgcgct
     8281 aagcaatgct gagtctctac aacgccagga gttgcgcgac aattttcata ccattaaaga
     8341 gaactggcat cgtttaatga atcgtcagcg gtggcttgat tactggcaaa atatttattc
     8401 gcgctcgctt agcgttcttc cctactttct gttattgccg cagtttatta gcgggcagat
     8461 caatctgggc ggactgatga aatcgcgcca ggcatttatg ctagtatcga acaatttaag
     8521 ctggtttatt tataaatatg acgaacttgc tgaactggct gcggttatcg atcgcttgta
     8581 tgagttccat caactcactg aacagcgccc tacgaataag cctaaaaatt gccaacatgc
     8641 ggtacaagtg gctaatgcga gtattcgtac gcctgataat aagatcatat tagagaactt
     8701 gaactttcat gtttcgccag gcaaatggct attactgaaa ggctactctg gtgcgggaaa
     8761 aaccacactg cttaaaacat tatcccactg ctggccgtgg tttaaaggtg atatttcttc
     8821 tcctgctgac agttggtatg tgtcacaaac accgttaatc aaaagcggct tactgaaaga
     8881 gattatttgt aaagcacttc ccctgcccgt agacgataaa tcgttgagcg aagtactgca
     8941 tcaggttggt ctggggaaat tggctgcgcg tattcatgac cacgatcgct ggggagatat
     9001 tctttccagc ggcgaaaaac aacgtatcgc cctggcacga ttaattttac gacgtccgaa
     9061 atggatattt cttgacgaaa ctacctctca tcttgaggaa caagaggcta tacgcttact
     9121 gcgtttagtg cgtgaaaaac tacccacaag cggcgtcatt atggttacac atcaacccgg
     9181 tgtctggaac ctggctgatg atatttgtga cattagcacg gttttataac aacaataaaa
     9241 gccctgctgt acggagatat aaataaatga agcgagttct tattcctggc gtcattttat
     9301 gtggcgctga tgtggcgcag gccgtcgatg acaaaaacat gtacatgcat ttttttgaag
     9361 agatgacggt ctatgctcct gtccctgtac ccgtaaacgg caacacgcat tacaccagtg
     9421 aaagcatcga gcgtttaccg accgggaatg gcaatatcag cgatctgctg agaaccaacc
     9481 ctgcggtacg catggattca acgcaaagta cctcgttgaa ccagggagat attcgccctg
     9541 agaaaatctc tattcacggt gcgtcgccct accagaatgc ctatttgatt gacggtatta
     9601 gtgcaactaa taacctgaac ccagcgaatg agtccgatgc cagtagtgca accaatatta
     9661 gcgggatgtc acaggggtat tatcttgatg tcagcttact ggacaatgtg acgctttatg
     9721 acagttttgt gccggttgaa tttggtcgct tcaatggcgg ggtaattgat gcaaagatca
     9781 aacgcttcaa cgctgatgat agcaaggtga aattgggtta tcgcactacg cgttcggact
     9841 ggttaacatc gcatatcgat gagaataaca agagcgcatt taatcaaggc tcttcaggaa
     9901 gtacttatta ctccccagat tttaaaaaga acttttatac cttgtcgttt aatcaggaac
     9961 tcgctgataa cttcggcgtt accgccggtt tatcgcgccg ccagtctgat atcacccgcg
    10021 cggattatgt ttcgaatgac ggcattgtca ccggtcgggc acagtataaa aacgttatcg
    10081 atactgcatt gagcaaattt acctggtttg ccagcgaccg ctttacccac gatttaacct
    10141 taaaatatac cggctccagc cgtgattata ataccagcac cttcccgcag tctgatcgcg
    10201 aaatgggtaa taaatcctat ggtctggcat gggatatgga tacgcaactc gcatgggcca
    10261 aactacgtac caccgttggt tgggatcata ttagtgatta tacccgtcac gatcatgaca
    10321 tctggtacac cgaactttca tgtacatatg gtgatattac agggcgttgt acccgtggcg
    10381 gattaggaca catttcccag gctgtagata attacacctt caaaacacgc ctggactggc
    10441 aaaaattcgc cgtgggtgat gtttcgcatc aaccctactt cggcgcggaa tacatctatt
    10501 ccgatgcatg gactgaacgc cataaccagt ctgaatccta tgtgattaat gctgcaggaa
    10561 agaaaactaa ccataccatt taccataaag gtaaaggcag cctgggaatt gacaactaca
    10621 cgctgtatat ggcggagcac attagctggc ggaatgtgtc gttaatgccc ggtgtgcgtt
    10681 atgactatga caactatctg tcaaaccaca atatctcccc gcgctttatg acggaatggg
    10741 atatttttgc tgatcaaacc tcaatgatta ccgccggtta taaccgttac tatggcggga
    10801 atattcttga tatgggatta cgtgatatcc gcaatagctg gacggaatcg gtatcaggta
    10861 ataaaactct gacgcgttat caggatttga aaacgcctta taacgatgaa ctggcaatgg
    10921 gattgcagca gaaaatcggt aagaacgtta ttgcgcgtgc aaactatgtt taccgtgaag
    10981 cgcatgatca aatcagcaaa agcagtcgta ccgacagcgc gactaaaacc accattactg
    11041 aatataacaa cgatggcaaa accaaaacgc attcgttcag cctcagtttt gaactggccg
    11101 aacccctgca tatccgccag gtagatatta acccacaaat tgtctttagc tatatcaaga
    11161 gcaagggcaa cttgtcgtta aacaatggtt atgaggagag caataccggt gataaccagg
    11221 tggtttataa cggtaatctg gtctcttacg atagtgttcc agtggcagat tttaataacc
    11281 cattaaagat ctccttaaac atggatttca cgcatcaacc gagcgggtta gtgtgggcga
    11341 atacgctggc ctggcaagaa gcgcgtaaag ctcgcattat ccttggtaag acaaatgcgc
    11401 aatacatcag cgaatattca gattacaagc agtatgttga cgaaaaactg gatagcagcc
    11461 tgacctggga cacccgcttg tcctggacgc cacaatttct gaaacaacaa aacctgacga
    11521 tcagtgccga tattctcaat gtactggata gcaaaaccgc tgttgataca acgaacaccg
    11581 gtgtggcgac ctacgccagt ggccgtactt tctggcttga tgtcagcatg aaattttaat
    11641 agttcggatt ttacttaacc gggtaaattc gcccggtttt cgcatggaga taattatgag
    11701 aaacctctgt ttcttactga cgttagtggc aactctgttg ctccccgggc ggctgattgc
    11761 cgccgcctta ccgcaggatg aaaagttaat taccgggcaa ctggacaatg gcttgcgata
    11821 tatgatttat ccgcatgctc aaccaaagga tcaggtaaat ttatggctgc aaattcatac
    11881 cggttcattg caggaagaag acaatgagcg cggcgtggct cattttgtag aacatatgat
    11941 gtttaacggc acaaaaacat ggccgggtaa taaagtcatc gaaacatttg agtcaatggg
    12001 cctgcgtttc ggtcgcgatg ttaatgccta taccagctat gacgaaacgc tgtatcaggt
    12061 gagtctgccg actacgcaga aacaaaatct gcaacaagtg atggcaatct tcagtgaatg
    12121 gagtaatgcc gcaacctttg aaatactcga agtagacgct gaacgcggcg taattactga
    12181 ggaatggcgt gcccatcagg atgcgaaatg gcgcacctct caggcgcgcc gccctttcct
    12241 gctggcaaat acccgtaatt tagaccgtga acctatcggc ctgatggata ctgtcgccac
    12301 ggtcacaccg gcacaattgc gccaatttta tcaacgctgg tatcaaccaa ataatatgac
    12361 ctttatcgtg gtcggcgata tcgacagtaa agaagcgctg gcgctgataa aggataattt
    12421 aagtaagctt ccggctaaca aagcagctga aaatcgcgtc tggccgacaa aagccgaaaa
    12481 ccacctgcgc tttaatatca ttaatgataa agaaaaccga gtgaacggca tcgcactcta
    12541 ttatcgcctg ccaatggtac aagtgaacga tgagcaaagc tttgtcgaac aagctgaatg
    12601 gagcatgtta gttcagctgt tcaatcaacg tctgcaggaa cgcatacagt cgggcgagtt
    12661 gaagactatt tctggcggca ctgcgcgcag cgttaaaatt gcacccgatt atcagtcgct
    12721 gtttttccgt gtaaatgcac gagacgataa tatgcaggat gctgcaaatg cattaatggc
    12781 agagttggca accattgatc agcatggttt ttctgctgaa gaactcgatg atgtcaaatc
    12841 tacccgcctc acctggctga aaaatgcggt tgatcagcaa gctgaacgtg atttacgtat
    12901 gctgaccagt cgcctggcat ccagctcatt aaataatacg ccgttcttgt cgccggaaga
    12961 gacatatcaa ctttcgaaac gtctgtggca gcaaattacc gtgcaaagtc tggcggaaaa
    13021 atggcagcag ttaagaaaga accaggacgc attttgggag caaatggtaa acaatgagct
    13081 tgccgccaaa aaagcattgt ctcttgcggc tatcctggcg ctggaaaaag agtacgccaa
    13141 caaaaagctg gcggcttaca tcttcccagg cagaaattta tcgttaacag tagacgctga
    13201 cccacaggcg gaaattagca gcaaagaaac gctggctgag aatctgacat cattaacact
    13261 ttccaatggt gccagggtta ttctggcaaa atccgcgggt gaagagcaaa agctacaaat
    13321 tactgccgta tctaataaag gcgatttaag tttccctgcg cagcaaaaat cacttatcgc
    13381 gctggcaaat aaagcagtaa gcggaagcgg cgttggtgag ctctcctctt ccagcctgaa
    13441 acgctggagt gcggaaaatt cggtaaccat gagcagtaaa gtcagtggca tgaatacgtt
    13501 gctctccgtt agcgcgcgga ctaataaccc tgaacctggt tttcagttga ttaaccagcg
    13561 aatcacccac agcacgatta acgacaatat ttgggcatcg ctacaaaatg ctcaaattca
    13621 ggcgttgaaa acgctcgacc agcgtccagc ggagaaattc gcccagcaga tgtatgagac
    13681 gcgctatgct gatgaccgca cgaaattact gcaagaaaat cagattgtac agtttactgc
    13741 cgcagatgcg ctggctgccg atcgccagtt gttttcatct ccagcggata tcacgtttgt
    13801 cattgtcggt aatgtctcag aagacaaact cgtggcgtta attacgcgtt acttaggatc
    13861 aatcaaacac tctgattcgc cattagccgc aggtaaacca ttaactcgcg cgacggacaa
    13921 cgcatcggtt actgtaaaag aacaaaatga acctgtggca caggtttcac agtggaagcg
    13981 ttatgattcc cggacacctg ttaatctggc gacgcgtatg gcgctcgatg cttttaacgt
    14041 cgcactggca aaagatctac gcgttaatat tcgtgaacag gcatctggag catacagcgt
    14101 ttcttctcgc ctctcggttg atcctcaggc caaagatatc agtcatttgc tggcttttac
    14161 ttgtcaacca gaacgacatg atgaactgtt aacgttagcg aatgaagtga tggttaagcg
    14221 tctggctaaa gggatcagtg agcaagaact gaatgaatac cagcaaaacg ttcagcgcag
    14281 cctcgatatc caacagcgta gcgttcaaca attagcgaac actattgtaa atagtcttat
    14341 tcaatatgac gatcctgcag catggactga gcaggagcaa ttgttgaaac aaatgacggt
    14401 agagaatgtt aacactgccg ttaaacaata tctttctcat ccggtaaata cttatactgg
    14461 agtattattg ccaaaataat aacagccccg tcaacacatc gttggcgggg attttagcaa
    14521 tattcgccat ttttatgtaa taattttata aatgcgttca aaataataac caggtactaa
    14581 tagtgatatt ttaaggtctg atttttacgt gaaaattcag gaggcacaga atgcgcataa
    14641 aaataacagc ataaaacacc ttaccaccac ccaagaattt catattatat tgtttttcaa
    14701 tgaaaaaata ttattctcgt aatatctcac gataaataac attaggattt tgttatttaa
    14761 acacgagtcc tttgcacttg cttactttat cgataaatcc tactttttta atgcgatcca
    14821 atcattttaa ggagtttaaa atggataaga agcaagtaac ggatttaagg tcggaactac
    14881 tcgattcacg ttttggtgcg aagtctattt ccactatcgc agaatcaaaa cgttttccgc
    14941 tgcacgaaat gcgcgacgat gtcgcattcc agattatcaa tgacgaatta tatcttgatg
    15001 gcaacgctcg tcagaacctg gccactttct gccagacctg ggacgacgaa aatgtccaca
    15061 aattgatgga tttatccatt aacaaaaact ggatcgacaa agaagaatat ccgcaatccg
    15121 cagccatcga cctgcgttgc gtaaatatgg ttgccgatct gtggcatgcg cctgcgccga
    15181 aaaatggtca ggccgttggc accaacacca ttggttcttc cgaggcctgt atgctcggcg
    15241 ggatggcgat gaaatggcgt tggcgcaagc gtatggaagc tgcaggcaaa ccaacggata
    15301 aaccaaacct ggtgtgcggt ccggtacaaa tctgctggca taaattcgcc cgctactggg
    15361 atgtggagct gcgtgagatc cctatgcgcc ccggtcagtt gtttatggac ccgaaacgca
    15421 tgattgaagc ctgcgacgaa aataccatcg gcgtggtgcc gactttcggc gtgacctaca
    15481 ccggtaacta tgagttcccg caaccgctgc acgatgcact ggataaattc caggccgaca
    15541 ccggtatcga catcgacatg cacatcgacg ccgccagcgg tggcttcctg gcaccgttcg
    15601 tcgccccgga tatcgtctgg gacttccgcc tgccgcgtgt gaaatcgatc agtgcttcag
    15661 gccacaaatt cggtctggct ccgctgggct gcggctgggt tatctggcgt gacgaagaag
    15721 cgctgccgca ggaactggtg ttcaacgttg actacctcgg tggtcagatt ggtacttttg
    15781 ccatcaactt ctcccgcccg gcgggtcagg tgattgcaca gtactatgaa ttcctgcgcc
    15841 ttggtcgtga aggctatacc aaagtacaga atgcttccta ccaggtcgct gcctatctgg
    15901 cggatgagat cgccaaactg gggccgtatg agttcatctg tgccggtcgc ccggacgaag
    15961 gcatcccggc ggtttgcttc aaactgaaag agggtgaaga tccgggatac accctgtacg
    16021 acctctctga acgtctgcgt ctgcgcggct ggcaggttcc ggccttcact ctcggcggtg
    16081 aagccaccga catcgtggtg atgcgcatta tgtgtcgtcg cggcttcgaa atggactttg
    16141 ctgaactgtt gctggaagac tacaaagcct ccctgaaata tctcagcgat cacccgaaac
    16201 tgcagggtat tgcccagcag aacagcttta aacatacctg ataacgttta acggtaacgg
    16261 tgtcccgaaa cgaacccgtt tcgggataat ttccaaagtc tgttcactgg cattagcaac
    16321 ggaaaatatt gttctgaata cgcttcagaa caaaacaggt gcggttccga caggaatacc
    16381 gttttagggg gataatatgg ctacatcagt acagacaggt aaagctaagc agctcacatt
    16441 actcggcttt tttgccataa cggcatcgat ggtaatggct gtttatgaat accctacctt
    16501 cgcaacatcg ggcttttcat tagtcttctt cctgctatta ggcgggattt tatggtttat
    16561 tcccgtggga ctttgtgctg cggaaatggc caccgtcgac ggctgggaag aaggtggtgt
    16621 cttcgcctgg gtatcaaata ctctggggcc gagatgggga tttgcagcga tctcatttgg
    16681 ctatctgcaa atcgccattg gttttattcc gatgctctat ttcgtgttag gggcactctc
    16741 ctacatcctg aaatggccag cgctgaatga agaccccatt accaaaacta ttgcagcact
    16801 catcattctt tgggcgctgg cattaacgca gtttggtggc acgaaataca cggcacgaat
    16861 tgctaaagtt ggcttcttcg ccggtatcct gttacctgca tttattttga tcgcattagc
    16921 ggctatttat ctgcactccg gtgcccccgt tgctatcgaa atggattcaa agaccttctt
    16981 ccctgacttc tctaaagtgg gcaccctggt tgtatttgtt gccttcattt tgagttatat
    17041 gggcgtagaa gcatccgcaa cccacgtcaa tgaaatgagc aacccagggc gcgactatcc
    17101 gttggctatg ttactgctga tggtagcggc aatctgctta agctctgttg gtggtttgtc
    17161 tattgcgatg gtcattccgg gtaatgaaat caacctctcc gcaggggtaa tgcaaacctt
    17221 taccgttctg atgtcccatg tggcaccgga aattgagtgg acggttcgcg tgatctccgc
    17281 actgctgttg ctgggtgttc tggcggaaat cgcctcctgg attgttggtc cttctcgcgg
    17341 gatgtatgtc acagcgcaga aaaacctgct gccagcggca ttcgctaaaa tgaacaaaaa
    17401 tggcgtaccg gtaacgctgg tcatttcaca gctggtgatt acttctatcg cgttgatcat
    17461 cctcaccaat accggtggcg gtaacaacat gtccttcctg atcgcactgg cgctgacggt
    17521 ggtgatttat ctgtgtgctt atttcatgct gtttattggc tacattgtgt tggttcttaa
    17581 acatcctgac ttaaaacgca catttaatat ccctggtggt aaaggggtga aactggtcgt
    17641 ggctattgtc ggtctgctga cttcaattat ggcgtttatt gtttccttcc tgccgccgga
    17701 taacatccag ggtgactcta ccgatatgta tgttgaatta ctggtcgtta gtttcctggt
    17761 ggtacttgcc ctgcccttta ttctctatgc tgttcatgat cgtaaaggca aagcgaatac
    17821 cggcgtcact ctggagccaa tcaacagtca gaacgcacca aaaggtcact tcttcctgca
    17881 cccgcgtgca cgttcaccac actatattgt gatgaatgac aagaaacact aaccatgtct
    17941 gaatggttat aagacaaggg agcgataatt catcgctccc tttttcgtgc tcgctgtcgt
    18001 attgacttaa ccgggataag tacgagaatg agcgcacatc tgtttaccgg aaaccagcat
    18061 atatggatat ctgctcccga aacgagaaat tagcgattag aagaccagcg atactggttg
    18121 cactggcact tttactgtgt agttgtaaaa gcacgcctcc agagtccatg gtgacaccac
    18181 cagcaggttc aaagccacca gccacgacgc aacaatcgtc acaaccgatg cgtggcatct
    18241 ggctggccac ggtgtctcgc ctcgactggc caccggtttc ctcggttaac attagtaacc
    18301 ccaccagccg ggcccgtgta caacaacagg cgatgatcga caaactggat catctgcaac
    18361 gtctcggtat aaacacggtc tttttccagg ttaagccgga cggtaccgcc ctgtggccat
    18421 cgaaaatttt gccatggtcc gatcttatga ccggtaagat tggtgaaaat ccgggttacg
    18481 atccgctgca atttatgctc gacgaagccc acaagcgtgg gatgaaagta cacgcctggt
    18541 ttaaccccta tcgcgtatcg gttaatacga agcccggtac tatcagggaa ctgaatagca
    18601 ctctgtctca acaaccggcg agcgtctatg tgcaacaccg tgactggatc agaacgtctg
    18661 gcgatcgctt tgtcctcgac ccgggcatcc ctgaggttca ggactggatc acatcaatag
    18721 tcgcagaagt ggtttcccgc tatccggtag atggcgtgca gtttgacgac tatttctata
    18781 ctgagtcacc gggttcacgg ctaaatgata acgaaacgta ccgcaaatac ggaggcgcat
    18841 ttgcgtcaaa agcagactgg cggcgcaaca atactcagca gttaattgca aaagtatcgc
    18901 acaccattaa aagcattaag ccgggagtcg aatttggcgt tagcccggca ggcgtgtggc
    18961 gtaaccgatc acacgatccg ctcggttccg atacccgagg cgcggcagcc tatgacgaat
    19021 cctacgctga cacccgtcga tgggtggaac aaggattgct ggattacatt gctccccaaa
    19081 tttactggcc gttctcacgg agtgccgcgc gttatgacgt gttggcaaaa tggtgggcgg
    19141 atgtcgttaa accgaccagg acccgcctgt atatcggtat cgccttctat aaagtgggtg
    19201 aaccttcaaa gatagagcca gactggatga ttaacggcgg cgtaccggaa ctgaaaaagc
    19261 agctcgatct taacgatgcg gtaccagaaa ttagcggcac catcttgttc cgtgaggact
    19321 atctgaataa accgcagact caacaagcgg tcagctatct gcaaagtcgt tggggcagtt
    19381 aaactcccat gcttactttg taacaagccg gtgctttacc caccggcttg ttacaccttg
    19441 tgaaatatcc cactagtaat catgcttaca taagtcaaat taaccacgca attaaccaca
    19501 cttagttaaa cagctataaa tccctaataa aaatgtatga atatgaaaaa ccatacgata
    19561 ttcgctgtca ccgaaaaata attacgatgg gctatactct tctgaagaac agtagcgtag
    19621 attgaaactg tcctaagtcg cttatttctt ttcagtatat cttcatattt caggagaata
    19681 tggtgcagtt agtcaatctc gcacgttgcg ttttgattcc gtctgatttc ccagccttat
    19741 aagggtgtga attatggaga tgtattttaa aagaatgaaa gatgagtgga ccggacttgt
    19801 cgaacaggca gatccactca ttcgtgctaa agccgcggaa attgccgttg cgcatgctca
    19861 ttatctgagt atcgagtttt atcgaattgt ccgcatcgac ccgcatgccg aagaattctt
    19921 gagtaatgaa caagttgagc ggcagttgaa aagtgcgatg gaacgctgga ttattaacgt
    19981 gctttctgcc caggttgacg atgtcgaaag gctaatacaa atccagcata ccgtcgcgga
    20041 agtgcatgcc cgcataggaa ttccggtaga aattgtcgag atggggtttc gggtgctgaa
    20101 aaaaatcctc tatccggtaa tcttctcttc ggattattcc gccgcagaaa aacttcaggt
    20161 ctatcatttc tcgattaaca gtattgatat cgcgatggaa gtgatgaccc gcgcgtttac
    20221 ctttagtgac agtagtgcct caaaggaaga tgaaaattat cgtatcttct cgttactgga
    20281 aaacgccgaa gaagaaaaag aacggcaaat agcctcaata ctttcatggg aaatagatat
    20341 tatctataaa atcctgctgg attctgattt aggcagtagt ttgccattaa gccaggctga
    20401 ttttggcctg tggtttaacc ataaaggtcg acattatttt agtggcattg ccgaagtagg
    20461 ccatatctcc cgtctcattc aggatttcga cggtattttc aatcaaacca tgcgtaacac
    20521 caggaatttg aataacagaa gtctgcgggt gaaattttta ttacagataa gaaataccgt
    20581 atcgcagatt attaccttgc tgcgtgaatt gtttgaagaa gtatcgcgcc acgaagtcgg
    20641 tatggatgta ctaacgaaat tacttaaccg ccgtttcctg ccgactatct tcaaacgcga
    20701 aattgcccat gccaaccgga ccggtacacc gctgtcagtg ctgattattg acgttgataa
    20761 attcaaagag atcaacgata cgtggggcca taacactggc gatgaaattc tgcgtaaagt
    20821 ctctcaggcc ttttatgaca acgcccacag tagtgattat gtttttcgct acgggggcga
    20881 tgaatttatc attgttttga ctgaagcttc tgaaaacgaa acgttacgta ccgcagaacg
    20941 tattcgcagt cgggtggaga aaaccaaact gaaagccgca aacggcgagg atattgccct
    21001 ctcactttcc atcggtgccg ccatgtttaa tggtcatcct gactatgagc gcctcattca
    21061 aatagccgat gaagctctgt atatcgccaa aagacgaggt agaaaccgtg ttgaactctg
    21121 gaaagccagt ctttagatgc gccaggatgc agaggtaatc atgaagctaa ccgatgcgga
    21181 aaatgccgcc gatggcattt ttttccccgc ccttgaacaa aatatgatgg gcgcggtgtt
    21241 aattaacgaa aatgatgaag tgatgttttt caaccccgcc gcagagaagc tctgggggta
    21301 caaacgtgaa gaagtcattg gcaataacat tgatatgctg attccgcggg atttgcgtcc
    21361 tgcgcatcct gaatacattc gtcataaccg tgaaggtggt aaagcgcgcg tcgaggggat
    21421 gagtcgggag ctgcagctgg agaaaaaaga cggcagtaaa atctggaccc gttttgcgct
    21481 atcgaaagtg agcgccgagg ggaaagttta ttacctggcg ctggtacggg atgccagcgt
    21541 agaaatggcg caaaaagaac agacccgaca attgattatt gccgttgacc atctcgaccg
    21601 accggtgatt gtcctcgatc cggaacgcca tattgtgcag tgcaatcgcg catttaccga
    21661 aatgtttggt tactgcatta gcgaagccag cggtatgcag cccgatacac tcctgaacat
    21721 tcctgaattc cctgccgata accgcattcg tttacaacag ttgctatgga aaaccgcccg
    21781 cgatcaggac gaatttctgc tgttgacacg caccggtgaa aaaatctgga ttaaagcctc
    21841 tatcagcccg gtttatgacg tgctcgcgca tctgcagaac ctggtaatga ccttctcgga
    21901 tatcaccgaa gaacggcaga tccgccagct tgaaggcaat attctcgccg ccatgtgcag
    21961 cagcccgcca tttcatgaaa tgggggaaat catttgtcgt aacatcgaat ctgttctcaa
    22021 cgaatcgcat gtttcgctgt tcgcactgcg caacgggatg ccgatacact gggcgtcatc
    22081 ttcgcacggt gcagaagttc aaaatgcgca aagctggtca gcgaccattc gtcagcgtga
    22141 tggtgcgcct gcggggatcc tgcaaattaa aacctcgtca ggagcagaaa ccagcgcctt
    22201 tatcgaacgc gtggcagata tcagccagca tatggccgcg ctggcgctgg aacaggaaaa
    22261 aagccgtcag catattgaac aactcatcca atttgatccg atgaccggtc tgccaaatcg
    22321 caataacctg cacaattacc tcgatgacct tgtcgacaaa gccgtctctc cggtggtgta
    22381 tctcatcggt gttgaccata ttcaggatgt gattgatagc cttggctatg cgtgggccga
    22441 tcaggcattg ctggaagtgg tcaatcgctt tcgtgaaaaa ctcaaaccgg atcagtatct
    22501 ctgtcgtatc gaaggtacgc agtttgtcct cgtgagcctg gaaaacgacg tcagtaacat
    22561 tacccaaatc gccgatgagc tacggaatgt ggtcagcaag ccgataatga ttgacgataa
    22621 accattcccg ctaaccttga gtattggcat cagctacgac gtgggtaaaa accgcgatta
    22681 cctgctctcc actgctcaca atgcaatgga ttatattcgc aagaatggcg gtaacggctg
    22741 gcagttcttc agcccggcga tgaacgaaat ggtaaaagag cgtttggttt taggcgcagc
    22801 gctgaaagaa gcgattagca ataaccaact gaaactggtt taccagccgc aaatcttcgc
    22861 agaaacgggt gaactgtacg gcatcgaagc ccttgctcgc tggcacgatc cccagcatgg
    22921 tcatgtaccc ccttcacggt ttattcctct cgcagaagag attggtgaaa tcgaaaatat
    22981 tgggcgctgg gtcatcgcgg aagcttgccg tcagttagca gaatggcgta gccagaatat
    23041 tcatatcccg gcgttatccg tgaacttgtc ggcgctgcac tttcgcagta atcagctgcc
    23101 taatcaggtg tctgatgcaa tgcaagcctg gggtattgac ggccaccagc tgacggtaga
    23161 aatcacggaa agcatgatga tggaacacga taccgaaatc tttaagcgca ttcagatcct
    23221 gcgcgatatg ggcgtgggct tatcggtaga tgattttggc acgggctttt ccggattatc
    23281 ccgcttagtc agtcttccgg taacggaaat caaaattgac aaaagttttg tcgatcgttg
    23341 tctgacggaa aaacgcatcc ttgccttact tgaagccatt accagcattg ggcaaagcct
    23401 caatttaacc gtcgtggcgg aaggcgtcga aaccaaagag caatttgaga tgctacgcaa
    23461 gatccactgt cgcgttattc agggatattt cttttcccgc cccctacccg ccgaggaaat
    23521 tccaggctgg atgagcagcg tgttaccgct gaaaatctga caaattcctc tcgcccgcac
    23581 tcgcgggttt tcatttaacg tgacactgtc acttaaatac tgtgatttca gctacgattc
    23641 cgggagattc cttccttaac ttcccctctt gcaccaaaac tgcactacgc tgcacaaatg
    23701 ttgatcaaaa gttaaaaaat ataactttaa gccagcattc tggcgcttat cccggcatgg
    23761 catgagatct gcataagcgg aaagcgcagc aatttttgtc ttaatacagg aaccgtttat
    23821 gtcggatacc accgaactgg ttgatttagc cgtgatcttc cctgatctgg agatcgaatt
    23881 gaaatacgcc tgcgctgata acatcacagg taaagctatt taccagcaag cgcgttgtct
    23941 gttacacaag gatgcgatta ccgcgctggc gaaaagtatc agtatcgccc agctgtcagg
    24001 cttacaactg gtgatttacg atgcgtatcg cccacaacaa gcacaggcga tgttgtggca
    24061 agcctgccca gacccgcaat atgttgttga tgtgacggtc ggttctaatc acagccgtgg
    24121 cacggcgatc gacctgacgc ttcgtgatga gcacgggaac atcctggata tgggcgcagg
    24181 gttcgatgaa atgcacgagc gctcccatgc ctatcaccct tccgtcccgc ccgccgctca
    24241 gcgcaatcgc ctgttgctga atgcgataat gactggtggc ggttttgtcg gtatctccag
    24301 cgaatggtgg cacttcgaat taccccaggc agcgagttac cctctgctcg ctgatcaatt
    24361 ctcttgtttt atatcccctg gcacacagca cgtcagttaa tcaggaaact gtcatgaaga
    24421 gatcgatatc gtttcgtccc acattgctcg cgctcgtcct tgccacaact ttcccggttg
    24481 cgcacgccgc cgtaccgaaa gatatgctgg tgattggtaa ggccgccgat ccacaaaccc
    24541 tcgacccggc ggtaacaata gataataacg actggacagt gacctacccg tcttatcagc
    24601 ggctggttca gtacaaaacg gacggtgata aaggctcaac cgacgttgaa ggcgatctgg
    24661 caagtagctg gaaagcgtct gacgatcaaa aagagtggac gttcaccctg aaggacaatg
    24721 ctaaatttgc cgatggcaca cctgtcactg ccgaagcagt aaaactctct tttgagcggt
    24781 tactaaaaat cggccagggg ccagcagaag catttcccaa agatttaaag attgatactc
    24841 ccgacgaaca tacggtgaag tttaccctta gccaaccatt cgcaccgttc ctctacacgc
    24901 tagcgaatga cggtgcatcc attatcaatc cggcggtctt aaaggaacat gcagcggatg
    24961 atgctcgcgg cttcctcgcg caaaataccg ccggttccgg accatttatg ctgaaaagct
    25021 ggcaaaaagg tcagcaatta gttctggtgc caaatccgca ttaccccggc aataaaccga
    25081 actttaagcg agtatcggta aaaattatcg gtgaaagtgc ctcccgtcgc ctgcagctct
    25141 cccgtggcga cattgacatt gccgatgcgc tgccggtgga tcaactcaac gccctgaagc
    25201 aggaaaataa agtcaatgtg gcagagtatc cgtcactgcg cgttacctat ctgtatctga
    25261 ataacagcaa agcgcctctt aatcaggcgg atctgcgtcg ggccatttcc tggtctaccg
    25321 attatcaggg catggttaac ggcattctga gtggtaacgg aaaacagatg cgcggcccga
    25381 ttccggaagg catgtggggc tacgatgcga cggcaatgca atacaaccat gacgaaacga
    25441 aagccaaagc cgaatgggat aaagtgacga gcaaacccac cagcctgacg tttctctact
    25501 ccgataacga tccgaactgg gagcctattg ctctggcgac acaatccagt ctcaacaagc
    25561 tgggcatcaa tgtgaagctg gaaaagctgg cgaacgccac catgcgcgac agagtgggta
    25621 aaggtgatta cgacattgcg attggcaact ggagtccgga ttttgccgac ccgtatatgt
    25681 ttatgaatta ctggtttgag tcagacaaaa aaggtctgcc gggtaaccgc tcgttctatg
    25741 aaaacagtga ggtcgataag ttactgcgca atgcgcttgc gaccaccgac cagacgcagc
    25801 gtacccggga ctaccagcag gcacagaaaa tcgtcattga tgacgctgct tatgtgtacc
    25861 tgttccagaa aaactaccaa ctggcgatga acaaagaggt gaaaggcttt gtgttcaatc
    25921 ccatgctgga acaggtcttc aatatcaata ccatgagtaa ataacgtgac cttctggagt
    25981 attttacgcc aacgctgctg ggggctggtg ctcgtggtgg cgggcgtctg cgtgattacg
    26041 tttattatct cgcatctgat ccctggcgat ccggcgcggt tactggcggg tgaccgcgcc
    26101 agcgatgcta tcgtggaaaa tattcgccag caactgggac tggaccagcc actgtatgta
    26161 cagttttacc gctacttcag cgacctgttt catggtgacc tgggaacatc cattcgtacc
    26221 gggcgtccgg tgctggaaga gttgcgtata tttttcccgg cgacgctgga actggctttt
    26281 tgtgccctgc tgctggcact cctgattggc atcccgctgg gcatactctc tgcagtctgg
    26341 cgaaatcgct ggctggatca tctggtgcga ataatggcca ttaccggaat ctccacacct
    26401 gcgttctggc ttggactggg cgtcattgtg ctgttttatg gtcatctgca aattcttccc
    26461 ggcggcggaa ggcttgatga ctggctggat ccaccaacgc acgttaccgg cttttatctg
    26521 ctcgatgcgc tgcttgaagg caacggtgaa gtcttcttca atgcgttgca acatctcatc
    26581 ttaccggcat taacgctggc gttcgttcac ctgggaattg tcgctcgcca gatccgctca
    26641 gcgatgctgg aacaattgag tgaagactac attcgtaccg cccgggccag cggcttgccc
    26701 ggctggtata tcgttttatg ttatgcgcta cccaatgcgt tgatcccatc gattaccgta
    26761 ttgggtctgg cgctgggcga tttgttgtat ggcgcagtgc tcaccgaaac cgtttttgcc
    26821 tggcccggaa tgggtgcatg ggtagtaaca tcaatacagg cgctcgactt cccggcagtg
    26881 atgggctttg ccgtcgtggt ttcatttgct tatgtgctgg tcaacctggt ggtggatttg
    26941 ctctatttgt ggattgatcc gcgtatcgga cgtggaggtg gtgaatgatg ctaatgatgc
    27001 taagcgagga aacgtccgcc gtacgcccac aaaaacaaac gcgatttaac ggtgcaaaac
    27061 tggtatggat gctgaaaggc agtccgctca ccgtgaccgg cgcagtcatc attgtattaa
    27121 tgctattgat gatgattttt tcaccgtggc ttgcgacgca tgatcccaac gccattgatt
    27181 taaccgcccg ccttttgccg ccttctgcgg cgcactggtt tggcaccgat gaagtgggac
    27241 gcgatctgtt cagccgcgta ctggtcggca gtcagcaatc aattctcgcc ggattagtcg
    27301 tggttgccat tgcgggtatg attggttcgc tactcggatg tctatccggt gtgcttggcg
    27361 gacgcgcaga cgccatcatc atgcgcatca tggacattat gctgtcgatt ccttcgttgg
    27421 tactgacaat ggcactggct gccgctctcg ggccgagttt atttaacgcc atgctggcga
    27481 ttgctattgt gcgaattccc ttttatgtgc gcctggcgcg ggggcaagca ttagttgtac
    27541 gccagtatac ctatgttcag gcggcgaaaa cctttggtgc gtcgcgttgg catctgatca
    27601 gctggcatat tttacgtaac tccctgccgc cgctgatcgt gcaggcatcg ctggatatcg
    27661 gtagcgcgat tttaatggcc gccacgttgg gatttattgg cctgggtgct caacaaccga
    27721 gtgctgaatg gggcgcgatg gtagctaatg gtcgcaacta tgtactcgat cagtggtggt
    27781 attgcgcatt tccgggggca gcgattttgc ttaccgccgt cgggtttaat ctctttggtg
    27841 atggtattcg cgatctgctt gacccgaaag caggaggaaa gcagtcatga cccaacccgt
    27901 tctggacatt caacagctgc atttgagttt ccccggtttt aacggttatg ttcacgtgct
    27961 caacaatgtg tccttgcaga ttaaccgcgg tgaaattgtc ggtctggtgg gagaatccgg
    28021 ctcaggtaaa tcagtcaccg caatgctgat tatgcgtctg ctaccgacgg gcagttattg
    28081 cgtacatcgg gggcagattt cactgctggg agacgatgtt cttaacgctc gggagaaaca
    28141 gcttcgtcag tggcgcggcg cacgagtggc gatgatcttt caggaaccga tgaccgccct
    28201 caatccgaca cgtcgaatag gtcttcagat gatggacgtg atccgccatc atcaaccaat
    28261 aagtcgtcgg gaagccagag ctaaagcgat taccctgctg gaagagatgc aaatcccgga
    28321 tgccgtggaa gttatgtcgc gctatccgtt tgagctttca ggtggtatgc gccagcgggt
    28381 aatgattgcg ctggcattct cctgcgagcc gcaattgatt attgccgacg aaccgactac
    28441 ggcgctggac gtcacggtac agttgcaggt actgcgtttg cttaaacata aagctcgcgc
    28501 cagtggaact gcggtactgt tcatcagcca tgatatggcc gtggtgtcgc aactgtgcga
    28561 tagcgtttac gtgatgtatg ccggaagcgt aattgaaagc ggcgtgacgg cagacgttat
    28621 ccatcatccc cggcatccgt ataccattgg tttgctgcaa tgcgcaccag aacatggagt
    28681 accacgccag ccattacccg ccattccagg gacggtacca aacctcaccc atttgcctga
    28741 cggctgcgct tttcgcgatc gttgctatgc ggcaggtgca cagtgtgaaa acgtcccggc
    28801 gctgacagcg tgtggtgaca acaaccagcg ctgcgcctgt tggtatccgc agcaggaggt
    28861 cataagtatc tgacacgtta ttaacgttac gcgacgtcca tatcaatttc ccggcccgta
    28921 aaagctggct tggtaaaact acggaacatg ttcatgcgat taatggtatt gatttacaga
    28981 tccgccgtgg tgaaacctta gggatcgtcg gtgagtcagg ctgcggcaaa agcaccctcg
    29041 cacagctttt aatgggtatg ctgcaaccga gccacgggca atacatccgt tcaggatcac
    29101 aacgcattat gcagatggtg tttcaggacc cgctctcttc gcttaatccg cgcttatcgg
    29161 tgtggcgcat catcacagaa ccgctctgga tagctaagcg tagtagtgaa caacagcggc
    29221 gagcgttggc aaaggagttg gctgtgcagg tgggtattcg tccggagtat ctcgaccgcc
    29281 tgcctcatgc gttctccggc gggcagcggc aacgcatcgc cattgccatt gccagagcac
    29341 tctcttcgca gcctgacgtg attgtgcttg atgagccaac ctcggcgctg gatatctccg
    29401 tgcaggcgca gatcctcaat ttactggtaa cgctacagga aaatcacggg ctgacttatg
    29461 tgctgatttc acacaatgtc tcggtgatac gtcatatgag cgatcgggtg gcggtgatgt
    29521 atctcgggca gattgtagaa ctgggcgacg cgcagcaggt gctgacggca cctgcacatc
    29581 catacacccg attattgctg gattccctcc ccgccattga taaaccgctg gaggaagaat
    29641 gggcattatg taaaacggat ctgccgggaa accgcacgtt gccacaaggc tgttttttct
    29701 acgaacgttg ccctctggca acccacggat gtgaagtccg gcaatcatta gcaataaggg
    29761 aggacggacg tgagctccgg tgctggcggg cgctgtagag atgctaacaa cgcatcaggc
    29821 attcacgcgc ctgacgcgtc atccggcaat gctttacgat ttcaactggt aatccagcgt
    29881 aatttccgct ttcagtacct gagaaaccgg gcatcctgct tttgctttct ggatgatgcc
    29941 gtcaaaggta gaggcatcaa tacccggcac cgcaacttca ctcttcagtg cgattttcgt
    30001 aatcgcaaaa tcggcatcca ctttatccag cgacacatcg gcggtggtat caatcgatgt
    30061 tggcgtgaat cccgcttccc ccagcattaa tgaaagcgcc attgagaaac atgcggcatg
    30121 cgctgcgcca atcagttctt cagggttggt tcctttttcg ccttcaaaac gcgtgttaaa
    30181 tccatacggc tgttggttca gcacgccact ctgggtggat actgttccct tcccgcgttt
    30241 gatatcgcct tcccagtgtg cctgaccttt cttatggatt gtcattgttg ctctcctgtg
    30301 ggcttaaaaa ggaaattata gactacgtga gaaacagcac gaggataagc aggatattcc
    30361 gaataaaa
//
LOCUS       DAKISA010000043        30224 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.043, whole genome shotgun sequence.
ACCESSION   DAKISA010000043 DAKISA010000000
VERSION     DAKISA010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30224
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.043"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..737)
                     /locus_tag="OVW45_002049"
     CDS             complement(<1..737)
                     /locus_tag="OVW45_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HCV5526502.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQID"
     gene            complement(878..2911)
                     /gene="metG"
                     /locus_tag="OVW45_002050"
     CDS             complement(878..2911)
                     /gene="metG"
                     /locus_tag="OVW45_002050"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCV5526503.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3043..4152
                     /gene="apbC"
                     /locus_tag="OVW45_002051"
     CDS             3043..4152
                     /gene="apbC"
                     /locus_tag="OVW45_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCV5526504.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLNALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            4397..4678
                     /locus_tag="OVW45_002052"
     CDS             4397..4678
                     /locus_tag="OVW45_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCV5526505.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQPHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            4970..5512
                     /locus_tag="OVW45_002053"
     CDS             4970..5512
                     /locus_tag="OVW45_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCV5526506.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            5592..6266
                     /locus_tag="OVW45_002054"
     CDS             5592..6266
                     /locus_tag="OVW45_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCV5526507.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            6282..8762
                     /locus_tag="OVW45_002055"
     CDS             6282..8762
                     /locus_tag="OVW45_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCV5526508.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKHSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNLRRISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     STPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            8778..9812
                     /locus_tag="OVW45_002056"
     CDS             8778..9812
                     /locus_tag="OVW45_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HCV5526509.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     IATYEHNFNDSSCVSTNTITPMSSSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLAGSNTILKGAENNGVGFML
                     ESNGSPVTFLNITDNSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(9894..10232)
                     /gene="rcnB"
                     /locus_tag="OVW45_002057"
     CDS             complement(9894..10232)
                     /gene="rcnB"
                     /locus_tag="OVW45_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCV5526510.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(10451..11275)
                     /gene="rcnA"
                     /locus_tag="OVW45_002058"
     CDS             complement(10451..11275)
                     /gene="rcnA"
                     /locus_tag="OVW45_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HCV5526511.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDYEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            11396..11668
                     /gene="rcnR"
                     /locus_tag="OVW45_002059"
     CDS             11396..11668
                     /gene="rcnR"
                     /locus_tag="OVW45_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HCV5526512.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      11760..11856
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11891..12679
                     /gene="thiM"
                     /locus_tag="OVW45_002060"
     CDS             11891..12679
                     /gene="thiM"
                     /locus_tag="OVW45_002060"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCV5526513.1"
                     /translation="MQVDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGLGSFVPHFLDALWQLTQEVQA"
     gene            12676..13476
                     /gene="thiD"
                     /locus_tag="OVW45_002061"
     CDS             12676..13476
                     /gene="thiD"
                     /locus_tag="OVW45_002061"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCV5526514.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            13541..14359
                     /locus_tag="OVW45_002062"
     CDS             13541..14359
                     /locus_tag="OVW45_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCV5526515.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQVRLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            14411..15157
                     /locus_tag="OVW45_002063"
     CDS             14411..15157
                     /locus_tag="OVW45_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCV5526516.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(15131..16096)
                     /locus_tag="OVW45_002064"
     CDS             complement(15131..16096)
                     /locus_tag="OVW45_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCV5526517.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGEQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(16093..17097)
                     /locus_tag="OVW45_002065"
     CDS             complement(16093..17097)
                     /locus_tag="OVW45_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HCV5526518.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(17094..18371)
                     /locus_tag="OVW45_002066"
     CDS             complement(17094..18371)
                     /locus_tag="OVW45_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCV5526519.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSTDEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            18628..19680
                     /gene="fbaB"
                     /locus_tag="OVW45_002067"
     CDS             18628..19680
                     /gene="fbaB"
                     /locus_tag="OVW45_002067"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCV5526520.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGTTIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            19999..20853
                     /gene="gatY"
                     /locus_tag="OVW45_002068"
     CDS             19999..20853
                     /gene="gatY"
                     /locus_tag="OVW45_002068"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCV5526521.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTYAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADVFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            20882..22139
                     /gene="gatZ"
                     /locus_tag="OVW45_002069"
                     /pseudo
     CDS             20882..22139
                     /gene="gatZ"
                     /locus_tag="OVW45_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            22149..22601
                     /gene="gatA"
                     /locus_tag="OVW45_002070"
     CDS             22149..22601
                     /gene="gatA"
                     /locus_tag="OVW45_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCV5526522.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            22632..22916
                     /gene="gatB"
                     /locus_tag="OVW45_002071"
     CDS             22632..22916
                     /gene="gatB"
                     /locus_tag="OVW45_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCV5526523.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHSIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            22920..24275
                     /locus_tag="OVW45_002072"
     CDS             22920..24275
                     /locus_tag="OVW45_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCV5526524.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSTQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKTVLA
                     E"
     gene            24323..25363
                     /gene="gatD"
                     /locus_tag="OVW45_002073"
     CDS             24323..25363
                     /gene="gatD"
                     /locus_tag="OVW45_002073"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCV5526525.1"
                     /translation="MKSMVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTYPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            25469..26242
                     /locus_tag="OVW45_002074"
     CDS             25469..26242
                     /locus_tag="OVW45_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCV5526526.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(26324..27223)
                     /gene="yegS"
                     /locus_tag="OVW45_002075"
     CDS             complement(26324..27223)
                     /gene="yegS"
                     /locus_tag="OVW45_002075"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCV5526527.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDGIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCLLLR"
     gene            27629..27946
                     /locus_tag="OVW45_002076"
     CDS             27629..27946
                     /locus_tag="OVW45_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5526528.1"
                     /translation="MKKIAAISLISVFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            complement(28276..29637)
                     /gene="yegQ"
                     /locus_tag="OVW45_002077"
     CDS             complement(28276..29637)
                     /gene="yegQ"
                     /locus_tag="OVW45_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCV5526529.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(29784..30116)
                     /locus_tag="OVW45_002078"
     CDS             complement(29784..30116)
                     /locus_tag="OVW45_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCV5526530.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
BASE COUNT         7835 a         7024 c         7629 g         7736 t
ORIGIN      
        1 atatcaatct gttgaaggtg tattattaca tcagtagcat attccagacc gccttcctgg
       61 acgatctgat ccataacatc ctcacgcact ttatatcttg tgcatttcat ttcaagtgcc
      121 gtgagtacag ccattactgg tgatggtaac tgtgttgttt tcaggccgtc gatctgactg
      181 atcgcctggg cgacggtgtt ttgccattct aaagaacagg cgctgaagtc aaatgtggct
      241 gttttattat cccgttgcga cacactacag gctaagcgac gtaattgggt caatttgtct
      301 gcttgctgaa caggaactcc gggaaagcgg cgatgactta acgtggtgtg tacaacttcg
      361 ggcggaaaaa cgacagcaat atctttcgcc agccacggta aaacagcatc tgtatttttg
      421 tcaggcgcac gggtttctgc tacgaaagag ggttgttcct gaataatggt ggataagtcc
      481 gctaccttga gagagcccgt taccgtttgg gaaggttgca aagaattatc cttcgcttgc
      541 tccacatacc ccttcttcac cttctccgca atcagcttaa gctccgcttt tgccgctgcc
      601 gcagcatctg aaaaactttt tatctggctt tgccctttgg tgccaacttt tccccaactg
      661 atatgcaact cgtttccctg ttgctcaact gcccagaatt tatgtgattt ttcgtcctga
      721 tagataaagt gtctcattct gcatccctgt aattttatcc agttgaatgc agatgctacc
      781 agtatttatg cgggttagag agagacaaaa tcgcaagaaa acaactttag gaatttataa
      841 agaaaaggcg ctgtcgatgc agcgccttga agggggatta tttcacctga tggcctggtt
      901 tagcaccagc atccgggctt aacaggaaga tatctttccc gccaggacca gcagccatta
      961 ccatgccttc ggagatacca aagcgcattt tacgcggtgc caggttggcc accataatgg
     1021 tgtgacgccc gatcagtgcc tgcggatccg ggtaggcaga acgaataccg gagaagacat
     1081 tgcgtttttc accgccgaga tccagtgtca ggcgcagcag tttgtcagaa ccttcaacaa
     1141 actctgcgtt ttcgatcagc gccacgcgca ggtcaacttt tgcgaagtcg tcaaaggtga
     1201 tggtttcctg aatcggatca tctgccagtg ggccagtaac tggcgcggca gctgctttca
     1261 cttcttcttt agacgcttcc accagtgctt caacctgctt catatcgatg cggttataca
     1321 gcgctttgaa cggattcact ttgtggccca gcagcggttg ctggatacca tcccaggtca
     1381 gttctgtatt gaggaatgct tctgcgcgct cggtcagttt cggcagtacc ggcttcaggt
     1441 aagtcatcag cacgcggaac aggtttatgc ccattgagca aattgcctgc aggtcggcat
     1501 cgcggccttc ctgttttgcc accacccacg gagcctgctc gtcgacatag cggttagcca
     1561 gatcagccag cgccatgatt tcgcgcactg ctttaccaaa ttcgcggctt tcccacgctt
     1621 caccaatcac ttcagcggca tcagtgaagg ttttgtacaa ctgcgggtca gccagttcgc
     1681 ttgccagcac gccgtcaaaa cgcttgttga taaagcccgc attacgagag gccaggttaa
     1741 ccactttgtt cacgatatcg gcattcacac gctgaacgaa atcctccagg ttgagatcga
     1801 tatcatcaat gcgcgaagag agtttcgcag tgtagtagta acgcaggctg tcagcgtcaa
     1861 aatgattcag ccaggtgctg gctttaataa aggtgccgcg agacttggac atctttgcgc
     1921 cgttcaccgt cacatagcca tgaacaaaca ggttggtcgg cttgcggaag ttgctgcctt
     1981 ccagcatggc aggccagaac aggctgtgga agtaaacaat atctttaccg atgaagtggt
     2041 acagctcggc ggtggagtct ttcttccagt attcatcgaa gcttacgctg tcgccgcgct
     2101 tgtcgcacag attcttgaaa gaacccatgt agccaatcgg tgcgtccagc cagacgtaga
     2161 aatatttgcc cggcgcgttc ggaatttcaa aaccaaagta tggcgcgtcg cgggagatat
     2221 cccactgttg caggccggat tcaaaccact cctgcatttt atttgccacc tgctcctgca
     2281 acgcaccgct gcgggtccat gcctgcaaca tttcgctgaa agagggcaga tcaaagaaga
     2341 agtgttcaga atcacgcatt accggcgtag cgccagaaac caccgatttc ggctcgatca
     2401 attcagtcgg gctgtaggtc gcgccgcaga cttcgcagtt atcgccgtat tgatccgggg
     2461 atttacattt cgggcaggtg cctttcacaa aacggtccgg caggaacatg cctttttccg
     2521 gatcgtacag ctgagagatg gtgcggtttt taataaaacc gttctctttc aggcgagagt
     2581 agataagttc tgacaactgg cggttctctt cgctgtgcgt cgagtgatag ttgtcatagc
     2641 tgatgttaaa gcctgcgaaa tcagtctgat gctcctgact catttcgcca atcatctgct
     2701 ccggggtgat accaagctgc tgagctttca gcatgatcgg tgtaccgtgg gcatcgtcgg
     2761 cgcagatgaa gttgacctcg tggccgcgca ttcgctggta acggacccag acatcagcct
     2821 ggatgtgctc cagcatatgg ccgaggtgga ttgagccgtt agcgtacggc agtgcgcacg
     2881 tcaccagaat tttcttcgcg acttgagtca tagtaggcat tacttcttaa tagtgaaaag
     2941 gggttagata gtaccaaatg ggaaaatgtt aagtaagtac ttctctatat tcttcaggta
     3001 aactatcagc tgatggttta tctatacaaa aaaggagtcg ggatgaacga acaatcccag
     3061 gccaaatcac cagaagcctt gcgcgcaatg gtcgccggga cactggccaa ttttcagcac
     3121 ccaaccctga agcataacct caccacgctg aacgcgttgc accatgtcgc ctggatggac
     3181 gacacgttgc atgtggaact ggttatgcca ttcgtctggc atagcgcgtt tgaagagtta
     3241 aaagagcaat gtagcgccga actgctgcgt atcaccgggg cgaaagctat cgactggaaa
     3301 ctgtcgcaca atatcgccac cttaaaacgc gtgaagaatc agccgggtat taatggtgtg
     3361 aagaacatca ttgcggtcag ttccggtaaa ggcggcgtgg gtaaatcctc cacggcggta
     3421 aacctggcgc tggcactggc ggcggaaggg gcgaaagtcg gcattctgga cgccgatatc
     3481 tacggcccat caattcccac catgctgggc gcagaaaatc agcgtccaac ttcgccggac
     3541 ggtacccata tggcaccgat tatgtctcat ggcctggcaa ccaattcaat tggttatctg
     3601 gtcactgacg acaatgcgat ggtgtggcgt ggaccgatgg cgagcaaggc gttaatgcag
     3661 atgttgcagg aaaccttgtg gccggatctc gactatctgg tgcttgatat gccgccaggc
     3721 accggtgaca ttcaattgac gctggcgcaa aacattccag taactggtgc agtcgtggtc
     3781 accaccccgc aggacatcgc tttgatcgat gcgaaaaaag gcattgtgat gttcgagaaa
     3841 gtcgaggttc cggtgcttgg tattgtcgag aacatgagtg tgcatatttg cagtaactgt
     3901 ggtcaccatg aaccgatttt cggtaccggt ggtgcagaga aactggctga aaaataccac
     3961 acacagctgc tggggcagat gccgttacac atctccttac gtgaggatct cgataaaggc
     4021 acgccaacgg ttatcagccg tccggagagc gaatttaccg ctatctaccg ccagctggct
     4081 gaccgcgttg cagctcagct ctactggcag ggtgaagtca ttccaggcga gatttccttc
     4141 cgcgcggtct aacgcctatt aaccattctg gttattcttc atacaccgca gagatgttac
     4201 attgatgcgg tgtattagtt tttgccgcat taatgaacac ttatttaata ctgcgaatat
     4261 tctgcggacc ttcgtccgaa actctacaaa gtcgattgat catacaggca atgcttcatt
     4321 atcatccttc gtggctgtgt agtggatgta cagctatagt ttgttatgca ctcattctat
     4381 ccaggcataa ccttcaatga ataagtattg gctgtcagga attatttttc ttgcctatgg
     4441 attggcttct cccgcattct cttcagagac tgccacatta gcgattaatg gcaggatcag
     4501 tcccccaaca tgcagtatgg ctatggttaa tggtcaacct caaccgcatt gtggtcagtt
     4561 aacgtataat gttgacaccc gtcatctgtt ttcttcgccc gtcaaaggtg taacgacaga
     4621 agtggttgtt gcaggtagcg atagcaaacg tcgaatcgtg ttaaatcgct acgattaatt
     4681 tggcaacctg attttaattg catttagaaa gccgaaatca tttatataaa tgatttcggc
     4741 ttttttattg atatcaacaa taccatttac ataaatctac attaattgat agatgcgata
     4801 aatagttaat actcttaaat atttgttgta tttatattgc aatggttaat ttgttttaaa
     4861 taaataaaac aatgtgttta cattgcggta acttttttga tgtgagaaat ttgtttccaa
     4921 gagatacaga ctcttaacaa ccaaaaaaaa ttttattaag gaacatatta tgaaacgttc
     4981 aattattgct gccgctgtct tttcttcttt ttttatgagc gctggagtat ttgctgcaga
     5041 cgttgatacc ggaacattaa ctattaaggg gaatattgca gaatctccgt gtaaattcga
     5101 agcgggtggt gattcagtaa gtattaatat gccgactgta ccaaccactg tctttgaagg
     5161 taaagctaaa tattctacct atgatgatgc agtcggtgta accagcagca tgttaaaaat
     5221 tagctgcccg aaagaagttg ctggtgtaaa actctcgttg attaccaacg ataaaataac
     5281 cggtaacgat aaggcgatag ccagtagcaa cgataccgtg ggttactatc tctatttagg
     5341 tgataacagc gatgtcctgg atgtttctgc accttttaac attgagagtt ataaaacagc
     5401 ggaaggtcaa tatgctattc cgtttaaagc aaaatacctg aaactgacag ataactcagt
     5461 gcaatcaggt gatgtgttat cttctctggt tatgcgtgtg gcgcaggatt aataaataaa
     5521 attgtaagtt tctccaggta ataaacatgg ccgctatccc atggcggcct tttaatttaa
     5581 gaggcattaa aatgaaagga ttattatctt tactcatttt ttctatggtc cttcctgcac
     5641 atgccggaat tgttatctac gggacgcgca ttatttaccc ggcagaaaat aaagaagtga
     5701 tggtgcagtt gatgaaccag ggaaaccgtt cttcgctgct gcaggcgtgg attgatgatg
     5761 gcgatacgtc attaccacca gaaaaaattc aggttccttt catgttaacg ccaccagtgg
     5821 caaaaatagg ggcaaattcc gggcagcaag taaaaatcaa aattatgccg aataaactgc
     5881 ccactaataa agaaagcatt ttttatctga atgttctgga tattccacca aatagtccag
     5941 agcaagaagg taagaatgca ctgaagtttg cgatgcaaaa cagaattaag ttgttttacc
     6001 gaccagcggg tattgctccg gtaaataaag cgacatttaa aaaattgctg gtaaatcgca
     6061 gtggcaatgg tttggtgata aaaaatgact cagctaattg ggtgacgatt tcggatgtca
     6121 aagctaataa tgtcaaagtc aattatgaaa ctattatgat tgccccctta gaaagtcaga
     6181 gtgttaatgt caaaagtaat aatgcaaata actggcatct gaccattatc gatgaccatg
     6241 gcaactatat tagtgacaaa atttaattgc aggagctgcc tatgttgaga atgaccccac
     6301 ttgcatcagc aatcgtagcg ttattgctcg gcattgaagc ttatgcagct gaagaaacct
     6361 ttgataccca ttttatgata ggtggaatga aagaccagca ggttgcaaat attcgtcttg
     6421 atgataatca acccttaccg gggcagtatg acatcgatat ttatgtcaat aagcaatggc
     6481 gcgggaaata tgagattatt gttaaagaca acccgcaaga aacatgttta tcaagagaag
     6541 ttatcaagcg gttaggcatt aatagcgata acttcgccag cggtaagcaa tgtttaacat
     6601 ttgagcaact tgttcagggt gggagctata cctgggatat cggggttttt cgtctcgatt
     6661 tcagtgtccc gcaggcctgg gtggaagaac tggaaagtgg ctatgttcca ccggaaaact
     6721 gggagcgggg tattaatgcg ttttatacct cttattatgt gagtcagtat tacagtgact
     6781 ataaagcgtc gggtaatagt aagagtacat atgtacgttt taacagcggg ttaaatttac
     6841 tggggtggca actgcattct gatgccagtt tcagtaaaac aaataacaat ccaggggtgt
     6901 ggaaaagcaa taccctgtat ctggaacgtg gatttgccca acttctcggc acgcttcgcg
     6961 tgggtgatat gtacacatca agcgatattt ttgattctgt tcgcttcaga ggtgtgcggt
     7021 tgtttcgtga tatgcagatg ttgcctaact cgaaacaaaa ttttacgcca cgggtgcagg
     7081 ggattgctca gagtaacgcg ctggtaacta ttgaacagaa tggttttgtg gtttatcaga
     7141 aagaggttcc tcctggcccg tttgcgatta cagatttgca gttggccggt ggtggagcag
     7201 atcttgatgt cagcgtgaaa gaggcggacg gttcggtaac cacctatctg gtgccttatg
     7261 cagcggtgcc aaatatgctg caacccggcg tgtcgaaata tgattttgcg gcgggtcgta
     7321 gccatattga aggggcgagc aaacatagtg attttgtcca ggcgggttat cagtatggtt
     7381 ttaataactt attgacgttg tatggtggct cgatggttgc gaataattat tacgcgttca
     7441 ctttggggac tggctggaat acacgcattg gtgccatttc cgtcgatgcc acgaagtcgc
     7501 atagtaaaca agacaacggc gatgtgtttg acgggcaaag ttatcaaatt gcctacaaca
     7561 aatttgtgag ccaaacgtcg acgcgttttg gtctggcggc ctggcgttat tcgtcgcgtg
     7621 attaccggac atttaacgat cacgtttggg caaacaataa agataattat cgccgtgatg
     7681 aaaacgatgt ctatgacatt gccgattatt accagaatga ttttggtcgc aaaaatagct
     7741 tttccgccaa tatgagccag tcattgccag aaggttgggg gtctgtgtca ttaagtacgt
     7801 tatggcgaga ttactggggg cgtagcggca gtagtaagga ttatcagttg agttattcca
     7861 acaacctgcg acggataagc tataccctcg cggcaagcca ggcttatgac gagaatcatc
     7921 atgaagagaa acgttttaat atttttatat cgattccctt tgattggggt gatgacgttt
     7981 cgacgcctcg tcggcaaata tatatgtcta actcaacgac gtttgatgat caggggtttg
     8041 cctcaaataa tacgggatta tcaggaacag tagggagtcg ggatcagttc aattatggtg
     8101 tcaacctgag tcatcaacat cagggaaatg aaacgacagc tggagcgaat ttaacctgga
     8161 acgcgccggt ggcaacagtg aatggcagtt atagtcagtc gagtacttat cgacaggctg
     8221 gagccagtgt ttcagggggc attgtcgcct ggtcgggtgg cgttaatctg gcgaaccgtc
     8281 tttccgaaac gtttgctgtg atgaatgcgc caggaattaa agatgcttat gtcaatgggc
     8341 aaaaatatcg cacaacaaac cgtaatggag tggtggtata cgacggaatg acaccttatc
     8401 gggaaaatca cctgatgctg gatgtgtcgc aaagcgatag cgaagcagaa ttacgtggca
     8461 accggaaaat tgccgcacct tatcgcggcg cggttgtact ggttaatttt gataccgatc
     8521 agcgcaagcc ctggtttata aaagcgttaa gagcggatgg gcaaccatta acgtttggtt
     8581 atgaagtcaa tgatatccac ggtcataata ttggcgttgt cggccaggga agccagttat
     8641 ttattcgcac caatgaaata ccgccatcgg ttaatgtagc aattgataag caacaaggac
     8701 tttcatgcac aatcaccttc ggtaaagaga ttgatgaaag tagaaattat atttgccagt
     8761 aatagtcagg tggttctatg gaaattcgca taatgctatt tatattaatg atgatggtta
     8821 tgcctgtgag ctatgcggca tgttatagtg agttatctgt tcagcacaat ttggttgttc
     8881 agggggattt tgcacttact caaacacaaa tagcgacata tgagcataat tttaatgatt
     8941 cgtcatgcgt gagtacaaat actatcaccc ctatgagttc gtcggatatt attgttgggc
     9001 tttataacga taccataaaa ttaaatttac attttgaatg gaccaataaa aacaacatca
     9061 cgttgtcaaa taatcagacc agtttcacca gtggttattc agttacggtg acacctgcgg
     9121 ccagtaatgc aaaagtgaat gtttctgcgg ggggcggcgg ttcagtgatg attaatggtg
     9181 ttgcgacatt atccagtgct tcatcatcga cacgcgggag tgccgcagta caatttctac
     9241 tgtgtttatt aggtggcaag tcatgggatg catgtgtaaa tagctacaga aatgcattgg
     9301 cacaaaatgc aggtgtctat tcctttaatc tgacattgtc atacaacccg ataaccacaa
     9361 cctgcaaacc ggacgattta ttaattactt tagacagtat tcccgtttca caattaccag
     9421 ccacaggtaa caaagcaaca ataaatagta aacaagggga tattattctg cgttgtaaaa
     9481 atttattagg tcaacaaaat caaacatcac ggaaaatgca ggtgtattta tcaagttctg
     9541 atttgttagc cggcagtaac acgatcctga aaggtgcaga aaataatgga gtcggattta
     9601 tgcttgaaag caatggttct ccagtcacat ttttaaatat cactgataac agcaaaggat
     9661 acacaaattt aaaagaaatt gcggcaaagt caaaacttac agatacaacg gtttcaattc
     9721 cgataactgc cagttactac gtctacgata caaacaaagt taaatctggc gcactggagg
     9781 caaccgcatt aatcaacgtg aaatacgact aataacaagc aagacgagca agtggctaat
     9841 aataaaaaat aacaaggtgt ctgcatttcc ccctcatgat gagggggctt tttttagcga
     9901 tgataaaaaa tctcaccgtc gtaggcttta atgattttac cgtcggtgtc gctgatcaac
     9961 acgtacgcgc cacccatata ggtccagtgc gtcccggcat caggcgcggg caggttacgt
    10021 aactgccact gcttaatgtt gtactcatcg gtgcggtaca tctctgggac ggtatcacct
    10081 ggtttgaaat gcttaaagtc agcaatgaaa ctacttaact catacttaga aattcccgag
    10141 gtattggtcg aacccgcggt ggctggtgcg gcccaggcgg cactggtaac cagcaaaagc
    10201 acacccagca acatcttatt tttaatggtc ataaactctc cactttgggg atctgtaata
    10261 tcagtcgcat atctttcctg ccatacaata taactgacaa gcgatatttt gttagtgatc
    10321 ccatgatttc gtaaaaagtg taaatgaaag gatgccctaa aagcatcctt agcgagcaga
    10381 gagtcagcgg aaatagctga tttaagaatc atcctgggct gaagataaca tgcaagccta
    10441 aaggattgag ttatcgcatt atgcccatga agccatgtac gcccatatac acaccgacta
    10501 agccaatcaa cagactggaa aaataggggg cgcgtttagc gagagtgtta aatccgctcc
    10561 agcgttttgc gacctgctga acgctgattg ctgcgccaac gcctacggtg acaagcgtta
    10621 acgccaggcc aatgctgaaa ctgacgacca gtgttgcgcc cagtgtcagg gctttcaact
    10681 gaatgcatat caacagcacg gtaattgctg ccgggcaggg gataaggcca ccggttaagc
    10741 caaatagcaa aatttgccag ttggtgacct ctctaccatc aaagcgtcgt ttaatgtcat
    10801 tggcatgggc tcgtgcatgg gcatcctgat actcgccatg ttcgtgatgg tgatgatgtc
    10861 catggtcgtg gtgatcttcg tgatcgtgat gatgatgctc ataatcatac tcgtgcatat
    10921 tctccagcca gttgcgttcg ccgcgccagg tacgccagaa catccagaac gcggtgccaa
    10981 taatgatcac tgcggaaatc agctggagcc acggttctgc tgattgagca gtaaagcgct
    11041 tgctgatcac catcccgcca aaggcaatta accagaccac tgcggtatgc gaaatagttg
    11101 ctgccagtcc gagcatcacc gcttgtttaa tggtgccttt gatggcgatg ataaacgccg
    11161 ccatcatcgt ttttgagtgc cctggttcca ggccatgaag cgcaccaagt aagatggcgc
    11221 tggggatgaa gaaccaggcg tttccttgct gaagaagagt tgtaaattcg gtcatgataa
    11281 taattcttag tattaattcg gcaatctgat tctactcccc ccagtacctg atactacccc
    11341 ccagtagatt aatagtgcta tgatattttc atattcttgt taaccaggtg ttgccatgtc
    11401 tcatacaatc cgtgataaac agaaactgaa agcgcgtgcc agtaagattc agggccaggt
    11461 cgtggcgctc aagaaaatgc tcgacgagcc gcatgaatgc gctgcagttt tacaacagat
    11521 tgctgctatc cgtggcgcgg taaacggtct gatgcgggaa gtgattaaag gtcatctgac
    11581 ggaacacatc gttcaccagg gggatgagct aaaacgtgaa gaagatctgg atgtcgttct
    11641 gaaggtgctg gattcatata tcaaataatt tattaacgcg attgtaaaac tgccgttttt
    11701 cctcgtttac aacgcgtgcg ctggacatta ccatcctcct ctgcgattta tcatcgcaac
    11761 caaacgactc ggggtgccct tctgcgtgaa ggctgagaaa tacccgtatc acctgatctg
    11821 gataatgcca gcgtagggaa gtcacggacc accaggtcat tgcttcttca cgttatggca
    11881 ggagcaaact atgcaagtcg acctgctggg ttcagcgcaa tctgcgcacg cgttacacct
    11941 ttttcaccaa cattcccctc ttgtgcactg catgaccaat gatgtggtgc aaacctttac
    12001 cgctaatacc ttgctggcgc tcggtgcatc gccagcgatg gttatcgaaa ccgaagaggc
    12061 cagtcagttt gcggctatcg ccagtgcctt gttgattaac gttggcacac tgacgcagcc
    12121 acgcgctcag gcgatgcgtg ctgccgttga gcaagcaaaa agctctcaaa caccctggac
    12181 gcttgatcca gtagcggttg gtgcgctcga ttatcgccgc catttttgtc atgaactttt
    12241 atcttttaaa ccggcagcga tacgtggtaa tgcttcggaa atcatggcat tagctggcat
    12301 tgctaatggt ggacggggag tggataccac tgacgccgca gctaacgcga tacccgctgc
    12361 acaaacactg gcacgggaaa ctggcgcaat cgtcgtggtc actggcgagg tggattatgt
    12421 taccgatggc catcgcgccg ttggcattca cggtggcgat ccgttaatga ccaaagtggt
    12481 aggaactggc tgtgcattat cggcggttgt cgctgcctgc tgtgcgttac caggcgatac
    12541 gctggaaaat gtcgcatctg cctgtcactg gatgaaacaa gccggagagc gcgcagtcgc
    12601 cagaagcgag gggctaggca gttttgttcc acatttcctt gatgcgctct ggcaattgac
    12661 gcaggaggtg caggcatgaa acgaattaac gctctgacga ttgccggtac tgatccgagt
    12721 ggtggtgcgg ggattcaggc cgatcttaaa accttctcgg cacttggcgc ttatggttgc
    12781 tcagttatta ctgcactggt ggcgcaaaat acccgtggcg tacagtcggt gtatcgcatt
    12841 gagcctgatt ttgtcgccgc ccagctcgat tcggtgttca gcgatgtgcg aatcgatacc
    12901 actaaaatcg gtatgttggc ggaaaccgat attgttgaag cggtggcaga acggttgcaa
    12961 cgttatcaga tccaaaacgt ggtactcgac accgttatgc tggcaaaaag cggcgaccca
    13021 ctgctttcac cttcggcagt tgccacgcta cgcagtcgat tattgccaca ggtttcatta
    13081 ataacgccaa acttgcccga agcagccgcc ttgctcgacg cgccacacgc gcgcaccgaa
    13141 caggaaatgc tggaacaagg gcgatcgctg ttggcgatgg gctgtggcgc agtgctaatg
    13201 aaaggtggtc atctggatga tgagcaaagc ccggactggc tgtttacccg cgagggtgaa
    13261 caacggttta ccgcaccgcg cattatgacc aaaaacaccc acggtactgg ttgtacactc
    13321 tctgcggcgt tggctgcact acgcccgcgc catacaaact gggctgacac cgtacaggag
    13381 gcaaaaagct ggctttcatc ggcgttagcc caggccgaca cgctggaagt tggtcacggt
    13441 attggtccgg ttcaccactt ccacgcctgg tggtgagcac cgtactatag ctgctttggt
    13501 tattgtcagc ctttggcact gacattcagg gatgaaacca atgcagctaa gaattaccag
    13561 tcgaaaaaaa ctcaccgctc tattatgcgc tcttgggcta atttccatcg ttgcaattta
    13621 tccgcgtcag acggtgaatt ttttctattc gacagcagtt cagattacag actacatcca
    13681 cttctacggt tatcgcccgg ttaaatcttt cgctattcgt attcctgcca gttacaccat
    13741 tcacggaata gatgtttcgc gctggcagga gcggatcgac tggcagcgtg tggcaaaaat
    13801 gcgcgataac ggtattcgct tacaatttgc ctttattaag gcgacggaag gcgaaaagct
    13861 ggtggacccc tatttttcgc gtaactggca actaagccgc gaaaatggct tgctgcgcgg
    13921 ggcatatcat tatttctccc cgtcggtagc agcaccggtt caggtgcgat tatttctgca
    13981 aacggtagat ttctcacaag gcgatttccc tgcggtactg gacgtagaag agcgaggaaa
    14041 attatcggca aaagaattac gcaagagggt aagtcagtgg ctaaaaatgg tcgaaaaaag
    14101 tacggggaaa aagccgatta tttactcagg agccgttttt tatcacacga atctggcggg
    14161 ctatttcaat gaatatccgt ggtgggtggc tcactactat caacgtcgtc cggacaatga
    14221 cggcatggcc tggcgcttct ggcagcattc cgaccgtgga caggtagatg gcatcaatgg
    14281 tccggtggat tttaatgtgt ttaatggcac ggtggaggag ttgcagggat tcgttgatgg
    14341 gattaaagaa acgccttaaa taagaagcag tgatatactc ccaccagact ccctggcagg
    14401 aaaaagacaa atggaacaag cgcataccca gctgattgcc caactgaacg aacgtatttt
    14461 agcggcggat aacacgccgc tttatattaa gtttgccgaa acggtaaaaa atgccgtgcg
    14521 cagcggggtg ctggagcatg gcaatatttt gcccggtgag cgtgatttaa gtcagttaac
    14581 cggcgtttcg cgcattacgg tgcgcaaggc gatgcaggcg ctggaagaag agggcgtggt
    14641 gacgcgttcg cgcggttacg gcacgcagat caacaacatc ttcgaatact cgttgaaaga
    14701 agcgcgaggt ttttctcagc aggtggtgtt gcgcgggaaa aagcccgata cgctatgggt
    14761 taacaagcgt gtcgtgaaat gtcccgaaga agtcgcgcag cagctggcgg tcgaagcagg
    14821 aagtgatgtc tttttgctta agcgtattcg ctatgtcgat gaagaagcgg tatcgattga
    14881 ggaatcgtgg gttccggcgc atttaattca tgatgttgat gccatcggga tttcacttta
    14941 tgactatttc cgcagccagc acatttaccc acaacgtacg cgttcccgcg ttagcgcccg
    15001 gatgccggat gccgagtttc agtcacatat tcagttagat agcaaaatac cggtgctggt
    15061 gatcaagcaa gtggcgcttg accaacagca acggcctatt gagtacagca ttagccactg
    15121 tcgcagcgat ctatacgttt ttgtgtgcga ggagtagttc ctcgcgcgtt ggcgcacaat
    15181 caccgccccg atgcccgaca acccacgacg ccactgcatt gcccagtaat acggcatccg
    15241 ccagtggcag accagaggcc agcccggcaa gtacgccacc tgcatgactg tcgcccgccc
    15301 caatggtgtc tacaacttgc gttggaaatg ccggaatgca gccagaagcg tcgttgctga
    15361 aataccatgc gccttcttta tcgagacgaa cgatcaacgg cgcggcaaat ttctcctgcc
    15421 attgctcgcc aagtgttgtt atctctgcgg ataaagcaaa acgttcggcg gcaatctcag
    15481 cctcttgacg attgagcgac actaaaggtc gacaggccat gatccgcgcc agtaatgcat
    15541 ccgggatatc gccaatacgt gggccaaaat cgataaacgg cgtcacgtct tgcagctctt
    15601 ccagccattc cactaacaat tcgccgcagg gcgaggccag ttgataaccg gaaaaataga
    15661 gcaggctgcc aggcgcaacg gttaatcgcg ccagccactg gcgattccac tgattttcca
    15721 caccgctgaa tgacataaaa gtgcgttcgc catccggctc aaccagcgcc agacaccagc
    15781 cgttatcacc ttcggcgtta tcgatcaggc tgattaagcc ctcttttgcc atccggttgc
    15841 gaataatctc cgcccacacg ccctgaccga gcggcaaggc attacccgct tcgatgccga
    15901 ggcgctttaa cgccacggca atattcagtg cgcagccgcc aacgttaacg ctctgctgtt
    15961 tcagttcgat atcgcaccca cgccagggga gggcataagc gtcggcgatc acgtcaatga
    16021 ccgccgcgcc gacgaccatc accggctgac gcgtggttaa ttcaggcagc agcgtgtgta
    16081 atcgagcgcc gctcataccg cctcccgttg ttgacgatat ttcgccagcg ctgtggcata
    16141 gcggttgaaa tcaagctgat ttaccgcatc cagttccgcc tttaacgcag gatcgatagc
    16201 gttaacgcca tgcaacgcgc cgcaaattgc cgtcgccata gcaccgatgg tgtctgtgtc
    16261 gccgccaagg ttagcgcaca ggacggcgca gcgattcggg tcggtttgtg ctagttcaac
    16321 cagcgcaatg gcgcacggaa cggactcaat agtgctggta cctgcgccaa cgacctggta
    16381 aagctgttcg ctggcggatt cggtgccgtc ggcattgcgc acaattttca gcgcaatctc
    16441 cagccgcgcc gccagtgagg cgctgaacgt agtgatgcgt ttttgctgtg catgtcgcgc
    16501 aattgaaggc agggaatcaa caatcgctga ccagctttct ccgtcaatgg cacgagaaat
    16561 cgcccatgcg atgactaccg cgcctgcaac cgccagatcg gatttatggg tcgggctgga
    16621 cgccagcgct acatcatcaa taaaggaatc aacatcacgc gccggaagca aacaacctaa
    16681 tggcgagacg cgcatcgctg cgccgttggt cacgccgttg ttttccagtt cagcaacggg
    16741 tttaccgtcg cgaatggcgt taagcgcaat cttcgaggtc ggacctagta cgtttttgtt
    16801 aaaggcgtcg aaacgcagcg cccagtcgag aatattacgc ccaatcagat ccggatcgat
    16861 cttgccttca cgttccagta acgcatccgc cagacacagc gccatcgagg tatcgtcggt
    16921 gaattcggcg cggttaaaat aacaggccgc gttattctcc tttggtccag gaagaaaacg
    16981 gtcaatccag ccaaagtgtg ctttaacgcg gctgcgtggc caaagctcgg agggcatccc
    17041 catcgcatcc cctaacgcct gcccataaag agcaccgaga atacgttctg ttttcattta
    17101 acttcccctt gtgtcaacgc aatatcgcga tcatcgacct tgatagccgt aatttcgttg
    17161 tcggattcgc ggaaaaaaat catgaacagc acggcgataa tggcaatcat caccgcgccg
    17221 aaagtccaca tcccggacca gttgaaagtc agtccgttta ccggttcctg ataagcgaac
    17281 atcttttcca tcatcacacc gccaagacga tagccgagca aactgccgaa gccctggcag
    17341 cagagcgtga tcagcccctg cgcagcggta cgcatatgca cgggggcttt tttatcgaca
    17401 tagatgtaag cggtaacgta gtaaaaatcg taacttacgc cgtgaagcaa aataccgagg
    17461 aacagtaacg cgtaggtgaa atattcatcc gtactaccgt aaataaagaa gccatagcgg
    17521 atcgcagcgg tgaccagacc aagcaataat acctttttga taccaaagcg tttagtgaaa
    17581 aacggcaatg ccagcataaa gaagatttca gagaactggc cgagcgtcat ccagccggtg
    17641 gcgtttttca tgccaacttc ggtcagataa ccgttggcaa agatgtaata gaacgccagt
    17701 ggcatcgcaa acaggaatga acagaagaaa aagacgagga agtttttatc gcgcagcagg
    17761 atcagcgcat ccaggccgag catgacttta atatccattt tgccggtgct ttttggtggc
    17821 gtgtcgggca ggaaaaacgc aaacacaccg agcagagcag aacttccggc ggtaatcagc
    17881 agcgggatgt tagtcggtga gatatcggca taccccagta tttgcggcaa gaaaccacat
    17941 gccagaccgg aggcaatcca gccgatagtg cccatcacac gaatgcgcgg gaaatcacgc
    18001 tccacatccg gcacgttggc aaaagcgatg ctgttagtca gcgcaatggt cggcatatag
    18061 gttagagagt aagccagcag taacggaaag aacccggcaa aagtggtctg ttgcgcagcg
    18121 aaatacatca gcagcgcgcc tgcgaacatc aataccgcca gcactttttg cgccgagaaa
    18181 aagcggtcag tgatggagcc aaccagaatt ggcgacagga tcgccgcaat ggcggtacag
    18241 gcatacgacc agccaatttc tccggcacta aaaccgcttt tacttaacca caaccacaat
    18301 ggcacaaacc acgcgcccca gataaaccat tcaacaaaca tcatgaacga cagctttgct
    18361 gttgttttca tcttttaatt ccttcatgac aggtaatgta aggtacgcga tgacaatacc
    18421 acttaacaat accttttaaa tacctttgcg gtgaaaactt gaccgcgcta acattttttc
    18481 tgatgaatcg agccaacaga aaacgctgaa aaaacatcca aaagatggaa aaactcgccc
    18541 gtaacgacca ggattatgat tgcccgcaaa aggcgggcca ggacaatcca tagccgatat
    18601 ccaatcggaa tttacgggag catagtaatg acagatattg cgcagttgct tggcaaagac
    18661 gccgacaacc ttttacagca ccgttgtatg acaattcctt ctgaccagct ttatctcccc
    18721 ggacatgact acgtagaccg cgtaatgatt gacaataatc gcccgccagc ggtgttacgt
    18781 aatatgcaga cgttgtacaa caccgggcgt ctggctggca caggatatct ttctattctg
    18841 ccggttgacc agggcgttga gcactctgcc ggagcttcat ttgctgctaa cccgctctac
    18901 tttgacccga aaaacattgt tgaactggcg atcgaagcgg gctgtaactg tgtggcgtca
    18961 acttacggcg tgttggcgtc ggtatcgcgg cgctatgcgc atcgcattcc atttctcgtc
    19021 aaacttaatc acaacgagac gctaagttac ccgaatacct acgatcaaac gctgtatgcc
    19081 agcgtggagc aggccttcaa catgggcgcg gtggcggttg gtacgactat ctattttggc
    19141 tcggaagagt cacgtcgcca gattgaagaa atttctgcgg cctttgaacg cgcgcacgag
    19201 ctgggcatgg tgaccgtgct gtgggcctat ttgcgaaact ccgcctttaa gaaagatggc
    19261 gttgattacc atgtttccgc cgacctgacc ggtcaggcaa accatctggc ggcaaccatc
    19321 ggtgcagata tcgtcaaaca aaaaatggcg gaaaataacg gcggctataa agcaattaat
    19381 tacggttaca ccgacgatcg tgtttatagc aaattgacca gcgaaaaccc gattgatctg
    19441 gtgcgttatc agttagctaa ctgctatatg ggccgggctg ggttgataaa ctccggcggt
    19501 gctgcgggcg gtgaaactga cctcagtgat gcagtgcgta ctgcggttat caacaaacgc
    19561 gctggcggaa tggggctgat tcttggacgt aaagcgttca agaaatcgat ggctgacggc
    19621 gtgaaactga ttaacgccgt gcaggacgtt tatctcgata gcaaaattac tattgcctga
    19681 tacgcctcat acgaacagca ccaccccgta ggacggataa ggcgtttaca ctgcatccgg
    19741 caacctatgc ctgatgcgac gcggaagcgt cttatcatgc ctacatagca ctgccacgta
    19801 tgtcggaaaa ggcgtttaca ccgcatccgg cataaaaaca cgcgcacttt gctacggctt
    19861 ccctatcggg aggccgtttt tttgcctttc actcctcgaa cagttttcat attgtcgttt
    19921 tgtgatcgtt atctcgacat ttaaaaacaa atcatttcat tatattttga aatcgaaaac
    19981 aaacgacagg atatgaaaat gtacgtggta tcgacaaagc agatgctgaa caacgcacag
    20041 cgcggcggtt atgcggttcc ggcattcaat attcacaatc tcgaaacgat gcaagtggtg
    20101 gtggaaaccg ctgccaacct gcatgcgccg gtcatcatcg ccggaacgcc tggcacattc
    20161 acttatgctg gtacagaaaa tctgttggcg ctggtcagcg cgatggcgaa gcaatatcac
    20221 catccactgg caattcatct cgaccatcac acgaaatttg acgatatcgc tcagaaggtt
    20281 cgttctggcg tgcgctcagt catgattgac gcctcgcatt tgccttttgc gcaaaatatt
    20341 tcacgggtca aagaggtggt ggatttttgc catcgctttg atgtcagcgt cgaagcggag
    20401 ctggggcaac ttggcggcca ggaagatgat gtgcaagtca atgaagccga tgttttttat
    20461 accaaccccg ctcaggcgcg tgaatttgcc gaggcaaccg gaattgattc cctggcggtc
    20521 gccatcggca cggctcatgg gatgtatgcc agcgcaccgg cgcttgattt ttctcgactg
    20581 gagaacattc gccagtgggt gaacttaccg ctggtgctgc atggcgcgtc agggttatcg
    20641 actaaggata ttcagcaaac catcaaactg gggatatgca aaatcaacgt tgcaacggag
    20701 ctgaaaaatg ccttctcgca ggcgttaaaa aattacctga ccgagcaccc tgaagcgacc
    20761 gatccccggg attatttgca gtcggctaaa tccgcaatgc gcgatgtggt gagcaaagtg
    20821 attgccgatt gtggctgcga gggcagggca taacgcacct gccatttaac aaggaaaaaa
    20881 catgaaaacg ttaattgccc ggcataaagc tggtgaacat atcggcatat gttcagtctg
    20941 ttctgcccat ccgttggtta tcgaagcggc gctggcattt gatcgcaaca gcacgcgcaa
    21001 agtgctgatt gaagcaacgt caaaccaggt caatcaattt ggcggttata ccggaatgac
    21061 accggcagac tttcgcgaat ttgtttttac gattgccgat aaagtcggat ttgcacgcga
    21121 acgcattatt ctcggcggcg atcatctggg gccaaactgc tggcagcaag aaaatgcgga
    21181 tgcggcgatg gaaaaatccg tcgagctggt aaaagcatat gttcgtgctg gcttcagtaa
    21241 aattcatctt gatgcgtcaa tgtcctgcgc gggggatccc ataccgttag caccagaaac
    21301 ggttgcggaa cgagctgctt tgctttgctg cggaaagtgt ggcgacaaat tgccagcgtg
    21361 agcaactgag ctatgtcatt ggcactgaag ttccggttcc gggcggtgag gccagcgcca
    21421 ttcagtcagt acacatcacc catgttgaag atgccgccaa tactttacgt acgcatcaaa
    21481 aggcctttat tgcccgtggg ctgacagagg cgttaacacg cgtgattgcc atcgtggtgc
    21541 agccgggcgt ggaatttgat cacagcaata ttatccatta tcagccgcag gaagcgcagc
    21601 cgctggcgca atggatagaa aacacccgaa tggtttatga agcacattct accgattacc
    21661 agacccggac ggcttattgg gaattagtcc gcgatcactt tgcaatattg aaagtcggtc
    21721 ccgcattaac ctttgcttta cgcgaggcga tatttgcact ggcacaaatt gagcaggaac
    21781 ttatcgcccc tgaaaatcgc agcggttgcc tggcggtaat tgaagaagtg atgctcgacg
    21841 aaccgcaata ctggaaaaaa tattatcgta cgggttttaa cgattcatta ctggatattc
    21901 gttacagcct gtcggatcgt attcgttatt actggccgca tagtcggatt aaaaatagcg
    21961 tcgaaacgat gatggtgaat cttgaaggcg tggacatccc actgggcatg attagtcagt
    22021 atcttcccaa acaatttgaa cgcattcagt ccggggaatt atcagcaata ccgcatcagc
    22081 tgattatgga taaaatttat gatgttttgc gcgcctatcg ctacggctgt gcggaataag
    22141 gacggtatat gactaacctg tttgttcgta gcggaatttc ttttgtcgat cgtagcgaag
    22201 ttttaaccca tatcggtaat gagatgctcg ccaaaggtgt ggttcatgat acctggccac
    22261 aggcattaat tgccagagaa gcagaattcc ctaccgggat aatgcttgag cagcacgcca
    22321 ttgcaatacc gcattgtgag gcgattcatg ctaagtcgtc agccatttat ctgttaaggc
    22381 caacaaataa agttcatttt cagcaagcgg atgatgataa cgacgtggag gtatcgttgg
    22441 ttattgcgtt gattgtggag aatccgcagc agcaattgaa acttttacgc tgtttatttg
    22501 gcaagttaca acagcccgat atcgtcgaga cactaatcac tcttcctgaa acccagttaa
    22561 aggaatactt cacaaagtat gttttagatt cagacgaata aatccctctg taacaataaa
    22621 aaggactatt tatgaaacgc aagattattg tcgcttgcgg aggcgcggtt gcgacctcta
    22681 cgatggcggc ggaagaaatt aaagagttgt gtcagaatca tagtattcct gttgaattaa
    22741 tccagtgtcg ggttaatgaa atagaaacct atatggatgg tgtgcatttg atatgcacca
    22801 ctgccaaagt ggatcgtagt tttggcgata ttccgttagt tcacggcatg ccttttattt
    22861 ctggtgtcgg tatcgaagca ttacaaaata aaattctgac tatcttacag gggtgaccta
    22921 tgttttcaga agtcatgcgt tatattctcg acctcggccc tacggtgatg cttccgattg
    22981 tcatcattat tttttctaaa atattaggca tgaaggcagg cgattgcttt aaagcgggtc
    23041 tgcatatcgg gattggcttt gttggcattg gccttgtgat tggcttaatg ctggattcca
    23101 ttggtccggc ggcgaaagcg atggcggaaa atttcgacct gaatctgcat gtggtcgatg
    23161 tcggctggcc gggctcttca ccaatgacct gggcgtcgca aattgcgctg gtggcgattc
    23221 cgattgcgat tctggttaac gtggcgatgt tactgacccg tatgacgcgg gtggtaaatg
    23281 ttgatatctg gaatatctgg catatgacct tcaccggcgc gttgctgcat ctggcaaccg
    23341 gttcatggat gatagggatg gcaggtgtgg taattcacgc ggcgtttgtt tataagctcg
    23401 gcgactggtt tgcccgcgat acccgaaatt tctttgagct ggaaggtatt gctattccgc
    23461 acggtacgtc ggcgtatatg gggccgattg cggtgctggt cgatgctatc atcgagaaaa
    23521 tcccaggcgt taaccgaatt aaatttagcg ccgacgatat tcagcgcaaa tttggtccat
    23581 ttggcgagcc tgtcaccgtg ggttttgtga tggggctgat tatcggcatc ctcgcgggtt
    23641 acgatgtcaa aggtgtattg cagctggcgg taaaaacggc ggcagttatg ctgctgatgc
    23701 cacgggtgat taaacccatc atggatggtt taacgcccat cgctaagcag gcccgtagtc
    23761 gtttacaggc gaagttcggc ggtcaggagt tcctgattgg cctggatccc gcattactgc
    23821 tggggcatac ggcggtggta tcggcaagcc tgatttttat cccgctcacc attttaattg
    23881 ctgtttgtgt tccgggtaat caggtactgc cgtttggcga tcttgccacc atcggcttct
    23941 tcgtggcgat ggcggtcgcg gtgcatcgtg gaaatctgtt ccgcacctta atctcgggtg
    24001 tcatcattat gagcatcacc ctgtggatcg cgacgcaaac tattggtttg cacacccaac
    24061 tggcggctaa tgctggggcg ttaaaagccg ggggtatggt ggcttcaatg gatcagggcg
    24121 gttctcccat tacctggtta ctgattcagg ttttctccac gcaaaatatt cccggtttca
    24181 ttattatcgg cgcaatttat cttaccggta ttttcatgac ctggcgtaga gcgcgtggtt
    24241 ttattaaaca agagaaaacc gttctcgcag aataattttt acctgagggg gaggttatcc
    24301 cccttaacaa tcaggagttt ttatgaaatc aatggtgaat gatactgatg gtatcgtgcg
    24361 cgttgcagaa agcgtcattc ctgaaattaa acatcaggat gaggtgcggg taaaaattgc
    24421 cagctcgggc ttatgtggtt ccgatttacc caggatattt aaaaatggtg cacattatta
    24481 tccaataacg ttaggccatg aatttagcgg ctatattgat gcggtgggat ccggtgttga
    24541 tgatttacac cctggcgatg cggttgcctg tgtgccgtta ttaccctgtt ttacttatcc
    24601 agagtgtttg aaagggtttt attcccagtg cgcaaaatat gattttattg gctcgcggcg
    24661 tgatggtgga tttgctgaat atattgtcgt taagcgaaaa aatgtctttg ctctacccac
    24721 ggatatgcct attgaggatg gggcttttat tgagccgatt accgttggcc tgcatgcttt
    24781 tcatttagcg caaggttgtg agaataaaaa cgttattatt attggtgccg gaaccattgg
    24841 cctgctggct attcagtgcg ctgtcgcgct gggagcaaag agtgtgacgg cgatcgacat
    24901 tagctcagaa aaactggcat tggcaaaatc tttcggtgcg atgcaaacat ttaacagtag
    24961 cgaaatgagc gcgccgcaaa tgcagagcgt tttacgcgaa ctgcgcttta atcagcttat
    25021 cctcgagacg gctggcgtac cgcaaactgt cgaactggcg gtagagattg ccggtcctca
    25081 tgcccaactg gcgctggtgg gcacgttgca tcaggatctg catttaacat cggcaacgtt
    25141 tggcaaaata ttgcgtaaag agctgacggt tatcggcagt tggatgaact attccagccc
    25201 ttggccgggg caggagtggg aaacggcgag ccggttgctg acagaacgta agttaagcct
    25261 ggagccatta atcgctcacc gtggaagctt tgaaagcttc acccaggcgg tgcgtgacat
    25321 cgctcgtaat gccatgccgg gcaaagtgtt gctcattcca tgaaaccgcg ggccagcgtg
    25381 atgctggccc ggtattgtgc aaaacagatc attcaccaat ggtccccctt cgtttacact
    25441 agccacaatt gaatatgggt aaatgacgat gaattcattc gagcgaagga ataagatcat
    25501 ccaattagtg aatgaacagg gaaccgtgct tgttcaggat ctggcgggag tatttgctgc
    25561 ctcggaagcg acaatccgtg ccgatttgcg ctttctcgaa caaaaaggcg tggttacgcg
    25621 ctttcatggc ggtgcggcga aaataatgtc cggtaatagt gaaaccgaga cccaggaagt
    25681 cgggtttaaa gagcgatttc agctcgccag cgcgccaaaa aacagaatag cgcaggcggc
    25741 agtcaaaatg atccatgaag ggatgaccgt tattctcgac agcggaagta cgacaatgct
    25801 tatcgccgaa ggattaatga ccgctaaaaa tatcacggtg attaccaaca gtctcccggc
    25861 agcgtttgcc ctttccgaaa ataaagacat tactctggtc gtctgtggtg gcacggtccg
    25921 ccataaaacg cgctcgatgc atggttccat tgccgagcgt tcattgcagg atattaatgc
    25981 cgatttaatg tttgtcggtg ctgatggtat tgatgcggtt aatggtatta cgacctttaa
    26041 tgaaggttat tcgattagcg gtgcgatggt caccgccgct aataaagtga ttgccgttct
    26101 cgattcatcg aaatttaacc gtcgcggttt taatcaggta ctgccaatcg aaaaaattga
    26161 cattattatt accgatgatg ctgtatcaga ggtggataaa ctggcattgc agaagacacg
    26221 ggtaaaatta attacggtgt agtattgttc tgtatgccgg ataaggtatt tacaccgcat
    26281 ccggcatttc ggattctccc tggttcaaga gagaatagtt tgattaacgc aataatagac
    26341 aatctggtgg taatcgacaa cgcaacgccg ccggaagtat ttcaatatga aagttttgtc
    26401 cgctcaacgg ttcgccatca agattaaaag tgatttcgtg tggggcttgt atatcaaacc
    26461 acgacgaagc gccttcgata atattcgggt tatcttcgtc agattttaag gttgatacga
    26521 gagccggaag aatttcatcg ccggtaaaaa tgcgcagttg cagcaagcca tcgttaatta
    26581 acgcgttcgg acacaattgc tgaccgccac cggcctgacg cccgttacca ataccaatga
    26641 ccagggcgtc accttgccag tgaaagtttt caccgcggat ttcacaacgg tccggttgca
    26701 gagtatccat gcgcattaag ccatgaatga tgtaagagac gccacccagc gcggctttta
    26761 atttttccgg cgtttctgtg gtaatacgcg tcccaaatcc gcctgtcgcc atattaataa
    26821 aacaggtttg tttgttgacc tgcgccatat ctatcgcaat ggcgttaccg gcaattgcca
    26881 gtttcagcgc cttatccagt gcctcaggaa tccctacact ggtggcaaaa tcattggcgg
    26941 ttcctaatgg caaaattccc agcgcgggta tgccatcccc ctcacactga atcaacgccg
    27001 tagaaacttc attaatggtg ccatcgccac caccggcaat caccgttgcg acgcccaact
    27061 tccgggcctc ctctacatat cgtgcggcat cgcctttctc ccaggtgacc cgcacatgga
    27121 tcgtcattcc ttcctcacgc aacagcataa ttgcttcgcg caagggtaga ttgtcagtac
    27181 ttttgccatt aagaatcagt aagctggcgg gaaattctgc catgatcgta tgtgccttta
    27241 tgattggtct gtagatagtg tagagcagaa aacaaaaggt ggagtcagaa caggatgaaa
    27301 gtcggaggat aaaaagtcag ttcaagtatg gaggattcag tggttaccac caggagcttg
    27361 caatggggac agcgaatact aacggctgcc accgataaat ttcaaaaaag agcatatacc
    27421 taatattcaa ctaaacagtg gcatcttcaa tataatatat taaagccccc atggagttac
    27481 cctgaagggc ctcaatgtcc gtaattccta cttatgtagg aaatgttgta cagaacattt
    27541 attataatcc tattcaatta taataatcat gccattatta tatttaaaca ctagagagtg
    27601 tcgttggtat ttaatggggg aaggtgagat gaaaaagata gctgctatat cattaattag
    27661 tgtttttctt atatctggtt gtgctgtgca taatgatgaa acaagtatcg gtaaatttgg
    27721 tcttgcatat aaaagtaata ttcagcgtaa actcgataac caatactaca ccgaagccga
    27781 agcttcttta gctaggggca gaatatctgg tgcagaaaat atagtaaaaa atgatgcagc
    27841 ccatttctgt gttactcagg gcaaaaaaat gcagatagtt gacctgaaga cagaaggtgc
    27901 aggattacat ggcgtcgctc gtctgacatt caaatgtgga gagtgagaat attttttggt
    27961 aagcatcaaa catgcgcgtt ctggttgtgc ttagccggaa cctgtgcgag cacgatgccg
    28021 ttacgtgaaa ggcatcgtgc tatgaaggga gattctatcg atgtggtcaa tggaagacgg
    28081 tgaccaggga tagggcttat gcataaaaaa taagcccgtg taagggagat tacacaggct
    28141 aaggaggtgg ttcctggtac agctagcatt ttatgggtta tgtttttcag cgaaacggat
    28201 gataacctta ataaatgcag ctgtatgtga tcggtttcta agaattttcc atccgggaaa
    28261 aataatcgaa attaatcact taccgtgtgg gttacgcgtg gtttccccgg agaaattacg
    28321 catcagcagc gcgtaattta gctcaagatc ctgcgggacc gggagccaca cagtataacc
    28381 atcgcctggt gcgaccggca tcgcttcacc tttggcgttt tccatgtgct caagggtaaa
    28441 gttaatgttg ccttgcggcg tcatcagctc aaggctgtcg ccaacggaga atttattttt
    28501 caccgctacc gctgcgaggt cccccttgcg ctcaccggta aactcaccaa caaactgctg
    28561 gcggtcagaa actgaataac cgtattcgta gttctgataa tcgtcgtgag tatgacgacg
    28621 caggaaacct tcggtatagc cacgatgcgc cagaccttcc agagtttcca gcaggctggt
    28681 atcgaacggt ttgcccgcag cggcatcatc gatagcttta cgataaacct gtgcggtgcg
    28741 tgcgcaatag tagaaagatt tagtacggcc ttcgattttc agcgaatgca cgcccatttt
    28801 ggtcaggcgt tctacatggg cgatggcgcg cagatctttc gagttcatga tgtaagtgcc
    28861 gtgctcatct tcaaacgcgg tcatatactc gcccggacgc tgggcttctt cgatcataaa
    28921 cactttgtcg gttggcgcgc cgatacctag cgtcggctca acattttgca ccggaatcgg
    28981 ctcgtacttg tgtacgatgt tgccaacgtc atcttctttc ccttcctgga cgttgtactc
    29041 ccagcggcag gcgttggtgc aggtaccctg gttcggatcg cgcttgttga tatagccaga
    29101 gagcaggcag cgaccggagt aggccatgca cagcgcaccg tgaacgaaga tctcgatctc
    29161 catatccggc acctgattgc ggatctcttc aatctcttcc agcgacagct cgcgagagag
    29221 gatcacgcgg gtcaggccca tttgctgcca gaatttcacc gtcgcccagt ttacggcgtt
    29281 agcctgcacc gagaggtgga tcggcatttc agggaagtgc tcacgcacca gcataatcag
    29341 ccctggatcg gacataatca gcgcatccgg ccccatttcc accaccggtt tcaggtcacg
    29401 gataaaggtt ttcagcttgg cgttgtgcgg tgcaatgttg accacgacat aaaacttttt
    29461 ccccagcgcg tgggcttcat tgatgccgag ctgaagattt tcgtggttga attcgttgtt
    29521 gcgcacacgc agggagtaac gcggctggcc cgcataaaca gcatctgcgc cataagcgaa
    29581 agcgtaacgc atatttttca gcgttcccgc cggggaaagg agttccggtt taaacataat
    29641 tctctcgttc tgatgacagg tcagatccgc ttcacctgat gaagcggtta ggggagtgcc
    29701 cccactttaa gggcgggcat tgtagcgctg tggggggatc tggtaaagcg ccggacgccg
    29761 acaacccacg cccggcatcg taattacgta ttgtctttca ccgtctggct tgtgccattg
    29821 gctttaacgg aggcaattcc ggtttcacga gattgcgcgg tggcgtacat ctggctggag
    29881 ccgataattt gatgattggc ggctttcaga ttgaaataga atttgccgtt acttgccgtt
    29941 tttttctcat agcgttcttc ttgcgggctg ttgctacgca ccgacgcgat gcccttttcc
    30001 gcagaggctt ttgatgtata cagctcgctg gtgaggatag tctcaccatt gcccgctttt
    30061 agcacaaacc ggaactgatt atcactgctc ttactgagtt caaaccaacc agccataatg
    30121 actccttgat tgaaattgcg tatcgaaaac agcatctaaa ggtgctgttt accgattaaa
    30181 gtatgagcga ttttctgatg cctgccaggg cggagagata atgt
//
LOCUS       DAKISA010000044        30074 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.044, whole genome shotgun sequence.
ACCESSION   DAKISA010000044 DAKISA010000000
VERSION     DAKISA010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30074
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.044"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            184..846
                     /gene="acrS"
                     /locus_tag="OVW45_002079"
     CDS             184..846
                     /gene="acrS"
                     /locus_tag="OVW45_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HCV5526531.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTFNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDGMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(849..1028)
                     /locus_tag="OVW45_002080"
     CDS             complement(849..1028)
                     /locus_tag="OVW45_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCV5526532.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVYP
                     LKLVNCDELNFQDRM"
     gene            complement(1112..1996)
                     /gene="yhdJ"
                     /locus_tag="OVW45_002081"
     CDS             complement(1112..1996)
                     /gene="yhdJ"
                     /locus_tag="OVW45_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCV5526533.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIVECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(2082..2378)
                     /gene="fis"
                     /locus_tag="OVW45_002082"
     CDS             complement(2082..2378)
                     /gene="fis"
                     /locus_tag="OVW45_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCV5526534.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(2404..3369)
                     /gene="dusB"
                     /locus_tag="OVW45_002083"
     CDS             complement(2404..3369)
                     /gene="dusB"
                     /locus_tag="OVW45_002083"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCV5526535.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(3698..4579)
                     /gene="prmA"
                     /locus_tag="OVW45_002084"
     CDS             complement(3698..4579)
                     /gene="prmA"
                     /locus_tag="OVW45_002084"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCV5526536.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     IVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYVDSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(4591..6042)
                     /gene="panF"
                     /locus_tag="OVW45_002085"
     CDS             complement(4591..6042)
                     /gene="panF"
                     /locus_tag="OVW45_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCV5526537.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(6032..6274)
                     /locus_tag="OVW45_002086"
     CDS             complement(6032..6274)
                     /locus_tag="OVW45_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCV5526538.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYHDIPLEDDDAA"
     gene            complement(6383..7732)
                     /gene="accC"
                     /locus_tag="OVW45_002087"
     CDS             complement(6383..7732)
                     /gene="accC"
                     /locus_tag="OVW45_002087"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCV5526539.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(7743..8213)
                     /gene="accB"
                     /locus_tag="OVW45_002088"
                     /gene_synonym="fabE"
     CDS             complement(7743..8213)
                     /gene="accB"
                     /locus_tag="OVW45_002088"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCV5526540.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(9191..10165)
                     /locus_tag="OVW45_002089"
     CDS             complement(9191..10165)
                     /locus_tag="OVW45_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCV5526541.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            10317..12257
                     /gene="csrD"
                     /locus_tag="OVW45_002090"
     CDS             10317..12257
                     /gene="csrD"
                     /locus_tag="OVW45_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCV5526542.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            12562..13605
                     /gene="mreB"
                     /locus_tag="OVW45_002091"
     CDS             12562..13605
                     /gene="mreB"
                     /locus_tag="OVW45_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCV5526543.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            13671..14774
                     /gene="mreC"
                     /locus_tag="OVW45_002092"
     CDS             13671..14774
                     /gene="mreC"
                     /locus_tag="OVW45_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCV5526544.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            14774..15262
                     /gene="mreD"
                     /locus_tag="OVW45_002093"
     CDS             14774..15262
                     /gene="mreD"
                     /locus_tag="OVW45_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCV5526545.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            15271..15864
                     /gene="yhdE"
                     /locus_tag="OVW45_002094"
     CDS             15271..15864
                     /gene="yhdE"
                     /locus_tag="OVW45_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCV5526546.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15854..17323
                     /gene="rng"
                     /locus_tag="OVW45_002095"
     CDS             15854..17323
                     /gene="rng"
                     /locus_tag="OVW45_002095"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCV5526547.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            17391..21191
                     /gene="yhdP"
                     /locus_tag="OVW45_002096"
     CDS             17391..21191
                     /gene="yhdP"
                     /locus_tag="OVW45_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCV5526548.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWHYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQYITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            21347..22792
                     /gene="tldD"
                     /locus_tag="OVW45_002097"
     CDS             21347..22792
                     /gene="tldD"
                     /locus_tag="OVW45_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCV5526549.1"
                     /translation="MSLNLVSEQLLAANSLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22920..23849)
                     /gene="aaeR"
                     /locus_tag="OVW45_002098"
     CDS             complement(22920..23849)
                     /gene="aaeR"
                     /locus_tag="OVW45_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCV5526550.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            24032..24235
                     /locus_tag="OVW45_002099"
     CDS             24032..24235
                     /locus_tag="OVW45_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCV5526551.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            24243..25175
                     /gene="aaeA"
                     /locus_tag="OVW45_002100"
     CDS             24243..25175
                     /gene="aaeA"
                     /locus_tag="OVW45_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCV5526552.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGGRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            25181..27148
                     /gene="aaeB"
                     /locus_tag="OVW45_002101"
     CDS             25181..27148
                     /gene="aaeB"
                     /locus_tag="OVW45_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCV5526553.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVFAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            27240..27512
                     /locus_tag="OVW45_002102"
     CDS             27240..27512
                     /locus_tag="OVW45_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCV5526554.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(27568..27831)
                     /gene="yhcN"
                     /locus_tag="OVW45_002103"
     CDS             complement(27568..27831)
                     /gene="yhcN"
                     /locus_tag="OVW45_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCV5526555.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDTAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(28196..28666)
                     /gene="argR"
                     /locus_tag="OVW45_002104"
     CDS             complement(28196..28666)
                     /gene="argR"
                     /locus_tag="OVW45_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCV5526556.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            29101..30039
                     /gene="mdh"
                     /locus_tag="OVW45_002105"
     CDS             29101..30039
                     /gene="mdh"
                     /locus_tag="OVW45_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCV5526557.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
BASE COUNT         7291 a         7430 c         7936 g         7417 t
ORIGIN      
        1 ctcgtaaatt gaaatatata ttcatgtggc gaatgtattt taggtaaata atatatatta
       61 tttatttgac gatttatagc aactcaatgt taccctctcg tataaataca cattagatga
      121 tagattaacc ttcgctattt tctcaccctg tgtcgaatat atttatttcc tgaataatta
      181 atcatggcaa aaagaaccaa agccgaagct ctgaagaccc ggcaagaact gattgaaact
      241 gccatcgccc agtttgcgca acatggcgta agcaagacga cgttcaacga cattgccgat
      301 gccgctaacg ttacgcgtgg cgctatctac tggcacttcg agaacaagac ccaactgttt
      361 aatgagatgt ggttgcaaca gccttcattg cgggagttaa tccaggaaca cttgacggct
      421 ggattagagc atgacccgtt tcaacaattg cgtgaaaaat tgattgtcgg cttgcaatat
      481 attgccaaaa ttccccgcca gcaggcgttg ctgaaaatct tatatcacaa atgtgaattt
      541 aatgatggga tgctggccga gggagtgata cgcgaaaaga tgggctttaa tccgcagact
      601 ctccgcgaag tattgcaggc gtgtcagcaa caaggttgtg tagcaaataa cctcgattta
      661 gatgttgtga tgattattat tgatggtgcc ttcagcggaa ttgttcaaaa ctggttaatg
      721 aatatggcgg gttatgatct ttataaacaa gcacccgcgc tggtcgataa cgtattaaga
      781 atgttcatgc cagatgaaaa cataacgaaa ttaattcatc aaacgaatga attaagtgtc
      841 atgtaaattc acattctgtc ctgaaaattc agttcgtcac agttaaccag ttttagtgga
      901 taaactgagt tcatattacg gcatttgtct gtttctgttg tcagcagttc aatccactgc
      961 atcagtaaag tatcaaacag aaaaacgaag acagcgaaaa cgacgagcca ccaatacttg
     1021 cgaatcatta cctgagcctg tgcatgttta accaagtctg gcaggaaata tacacgaaat
     1081 gaataatctg aaaaccaggc ttacacttcc tttactttgc aatgagatcg gggtcaactt
     1141 cgcttaaccg gcttcgtttt gacaggttgc cggtctttct ttttttcact ttcgccagtt
     1201 cttccgcgga gtaatgcgac gcgacatcca gccgtcgaag ccccattttg atgtactcgc
     1261 tgttgatctc aataccaatg aattttcgtc cgctggcgat ggctacggca ccggtagtaa
     1321 agctaccggc aaacgggtcg agaacgatat cgcctgggtt ggaagaggcg agaataatgc
     1381 gtttcagtaa ggcttccggt ttttgcgtcg ggtggttctc atattcatcc atcaaataac
     1441 gcacgcgcgg aaaatcccag acgttacccg gtactttttg atgattgtat ggctgtggag
     1501 gatttttgcg ataatcgatc aacgcgcgct gcgatccggt tttggcttct accagaatag
     1561 catcaccgtt gaatgtgtag ttctttgcgt ctttcaccat catcaggatg ggttcgtaca
     1621 tggagccgta gtgttttttc gcctgtactc cagaactgtc atatgaccag acgatgcgac
     1681 ttttgatggt aaaaagcttg cggcactgga gatcgataaa gggcatgttt tccgtactgt
     1741 tcataatgta catgctgccc tgctttttca gaacgcggtg gcactctaca atcacttcaa
     1801 acagccagtc gataaacaga tcttctttcc aggcttcgat cagaccatca aaatttttac
     1861 cgatgttata cggtgggtcg gcaaagatca gatcgacgct ttcagtaggt agctttttaa
     1921 gttcggcaag ggcatcaccg tgaataatgg tcttagcttc attaccaaac cgggtcggtt
     1981 cacatcctgt tctcatggtc actccctttg tgacacctat aaaaaaggcg cttccccatg
     2041 ccgagtagcg cctttttaat caagcattta gctaacctga attagttcat gccgtatttt
     2101 ttcaattttt tacgcagcgt accacggttg atgcccatca tcagcgcagc acgggtctgg
     2161 ttaccacggg tgtattgcat caccatgtcc aacaggggct gttctacttc agccagtacc
     2221 agctcataga ggtcattcac atcctgacca ttcagttgag caaaatagtt cttcagtgcc
     2281 tgtttaaccg agtcacgcag gggtttttgg gttacctgat cctgagagtt aacggtagaa
     2341 acggtcagta cgtcagaatt tacgcgttgt tcgaacatag ttctgtcagc tctttatttc
     2401 tgtttacgca aaattttcga agtatgcctc caacgcctcc agctgttcgc tggcatcctc
     2461 aatggcgttg aatgtgcgcc gaaactggtc atttggagcg tgttcctgga gataccagga
     2521 aacgtgttta cgtgcaattc ggtacccttt tgccggacca taaaagtcat gcagttcccg
     2581 aacgtgcgcg caaagcaagc gcttaacctc tgccaaaggc agcgggggca gcaactcccc
     2641 agtgtccaga taatgctgga tttcccgaaa gatccagggt cttccctgag ctgcgcggcc
     2701 tatcatcagg gcatccgccc ctgtatagtc gagcacagct ctggctttaa gcgggtcagt
     2761 aatgtcgcca ttcgcgataa ccggaatgga aactttctgc ttaactgccc gaatactgtc
     2821 gtactcagct tctccattga acaaacaggc gcgtgtacgg ccatgaatgg tcagagcctg
     2881 aatgccacag tcttcagcca gttgggcaat ctcttcgcag ttacggtgtt ccggtgccca
     2941 gccggtgcga atcttcaggg taacaggaac gtccactgca ttgacgacct cggtaaggat
     3001 cgatttaacg acatccgggt actgcaagag ggctgaacct gcgagcttgc gattcacttt
     3061 tttagccggg caacccatat tgatatcaat aatctgggca ccgctttcca cgttaatacg
     3121 tgctgcatct gccatttctt tcggatcgct accagcaatt tgcacggtgc gaataccggg
     3181 ttcatcaatg tgcaccatcc gtaaacgaga tttgtcgctt tcccaaacct gtgggttaga
     3241 agacatcatc tcggatactg tcaatccggc tcccatctcg tagcacaacg tccgaaaagg
     3301 tctgtctgta atgccagcca tgggcgctgc gatcaggcga tttctgagct gatattgtcc
     3361 gatgcgcatg agttaagaaa tgaccatact gtgactgcaa ggcggcgtat attacgcatt
     3421 ttttgcacga gatgaaaggc caaactttga ccaatcctct gagatggatc aaagaattgc
     3481 atttaaaatg agcgtggtgc gataattact cataaaaatc atcatattag aaaatagtga
     3541 ctaaaattta cactcaaaga aatttgagta agttctcaat ttttctttat gaatgaaaat
     3601 tttggcacgc aaatttgcgt aaataaccgg caaatttacg tgccttttgt gagcttgctc
     3661 gcacttcgcc ccgcgtcacc ctacggcgat gcgaaggtta attcttacga ccggtaatgc
     3721 ggcaccactc ttctttttcc acgaccgggt ccagtgcgaa gctgtcgaca taagcttcac
     3781 aaacgctctc tgcctggctt gccagaatac cggaaaggcc cagcaaaccg cctgaaaccg
     3841 gcaggacgct gattaacggt gccagttcac gtaatgggcc tgcaaggatg ttagcgacca
     3901 ccacgtcggc tttcatttct tctggctgat ctttcggtaa gtagagttcc agacggtcag
     3961 aaacgccatt acgttcggcg ttgtcgcggc tagcctgaat cgcctgcgga tcgatatcaa
     4021 taccaatggc ttttgctgca cccagtttca gcgccgcgat agccagaatg ccggaaccac
     4081 agccaaagtc gatgactatt ttaccggtta aatcgaggct gtcgagccac tgcaggcaca
     4141 gagaggtagt tggatgggta cccgtaccaa acgccagccc tggatctaac atcacgttga
     4201 cggcgttttc gtccggcaca tcacgccagc tagggcagat ccacagtcgt tcaccaaagc
     4261 gcatcgggtg gaaattatcc atccattcgc gctcccagtc tttatcttcc agttgttcga
     4321 ttttatgcgc gaagcctgcg ccgagcagcg gatggttttc cagaatcgcc accacgtcgt
     4381 tcatatcggt ttcagcgtcg aacagaccaa tcacatcggt gtcgccccac aggcgcgttt
     4441 cgcccggcag cggctcaaat actggcgtat cgtgggtatc ctgaaaagtg atagaaacgg
     4501 cacccgcttc catcagcgca tcgctaagat cttcagcgtt cgcgccggtg gtgttcagtt
     4561 tcagttggat ccaaggcatg gcaaaactct ttatttatca gtagtcaaaa cggtagcttg
     4621 cgggacggat gtaccgaaac ggtttccgac caggaaagcc agcaaactta gtagtaacga
     4681 gggcacgata gggtggaagc ccaggtactg aatattcagc gtcgcgagta cggcatacag
     4741 cacgccgcca acgatcatcg cacttagcgc gcctttggcg ttggcgcgtt cccagtaaag
     4801 acccagcacc agcggccaca ggaaaacggc ttccagccca ccgaaggcca gcaaattcag
     4861 ccagatgatc atttctggcg gcttccaggc ggcaagcagc agcaacgcgc cgagaactaa
     4921 cgtaattacc gccgacatcc gcttcagacg cgtctcgttt tgcatttgat ccggacggat
     4981 attcagatag agatctttaa tgatcgtagc ggaactttgt agcaattggg cgttaattgt
     5041 cgacatgatc gcagccatag gtgcagccag aaagatcccg gcagcaaacg gtggcagcac
     5101 ttttaccatt aacgtcggga tcaccagatc cggtacggtg agatcgggga tcactgcacg
     5161 acctaacgct ccggctaggt gcataccgaa catcaggatt gcgactacaa tcgtaccgat
     5221 gatgatcccc cggtgtaccg ctttgctgtc tttataagag atacagcgca ccgcagtatg
     5281 cggcaggcca atcacgccaa agcacaccag tacccagaac gacgtcataa aggcaggcga
     5341 cagaatatcg tcagcgcctt gtggcgtaac cagttgcgga tcgatggttt gcaaggtctg
     5401 tactgcgttg cttaagccgc cagcggcatg taccacgcca ataagcagca caacggtgcc
     5461 aatcagcatc acaagccctt gcatggtgtc gttcagcacg ctggcgcgaa agccgccaaa
     5521 ggcggtatac aacgcaatgc tgataccgaa aatcagcagc ccggtttcat aaggaatacc
     5581 cgccgcggtt tccagcaggc gcgcaccgcc gataaactgc acggtcattg ccccaacgaa
     5641 cgcaaccagc aaactcaaac tcgccagcca caccagaaga cgactctggt agcgggcaaa
     5701 cagcatgtcg ttcagcgtca ctgcattgta gcggcgcgca agaatcgcaa acttcttgcc
     5761 gagaataccg agtgaaagcc agactgcagg aagctgaatc atcgccagca atacccagcc
     5821 cagcccgtat ttataagcag ctcctggccc gccgataaac gaactggcac tgatataggt
     5881 cgcggtgagc gtcatcgcca gcacaatacc gcccatagag cggctgccga ggaaatactc
     5941 attaaggaag gtgccggtgc tccgtttacg catcgcataa accgagatac cgaacaccac
     6001 caccagatag gcgaccagcg gtagaattac ttcaagctgc atcgtcatcc tccagtggga
     6061 tatcgtgata gataaatttc accatcgccc agcacagtcc aataaacagt agcggcgtca
     6121 ggatgcaggc catctcaaac cagcgcggaa agccggtaaa accgggggca acgccaggta
     6181 agtaagcggc tactaaccaa actgccagat acaaaagggt cagccccagc gcccagcgcg
     6241 cctctttatg ggcctgaaca aaacgagtgt ccattttttg tccctgatgg gtgaagaaag
     6301 cgaggattgt accttatggg ggttgtcgat ccccagtaat aaaaaaggcc ggaaaatccg
     6361 gccttttgac gcttttgcag tcttattttt cctgaagacc gagttttttc tccagatagt
     6421 ggatgttagt gccaccatgc tggaagttct cgtcattcat gatgcggatc tgcagatcaa
     6481 cgttggtttt gataccgtcg atgatcagct cctgcagcgc attcttcatg cgggcaatcg
     6541 ccacgtcacg gttttcaccg tagcaaatca gcttaccgat cattgagtca tagtacggcg
     6601 gtacggtgta gcccgcgtag atatgagact cccaacgtac gccaaaaccg ccaggtgcgt
     6661 ggaaacgggt gattttgcct gggcttggca ggaaggtgtt cggatcttcg gcgttgatac
     6721 gacattccac cgcatggccg cgaacgtgaa cttcttcttg cttgatcgac agcggttgac
     6781 cggcagcgat acggagctgt tctttgatca ggtcaacgcc agtgatcatt tcggtaaccg
     6841 ggtgttctac ctgaatacgg gtgttcattt cgatgaaata gaactcgccg ttttcgaaca
     6901 gaaactcgaa agtacctgca ccgcgatagc cgatatcaac acacgcttta gcgcaacgtt
     6961 cgccgatgta gcgacgcagt tccggggtaa tgcccggtgc tggcgcttct tcgaccactt
     7021 tctggtggcg gcgctgcatg gagcagtcac gttccgccag atagatagcg ttgccctgac
     7081 cgtcagccag tacctgaatc tcgacgtggc gaggattttc caggtatttc tccatgtaaa
     7141 ccatatcgtt gctgaaagca gctttcgctt ccgcacgggt catggagatg gattgtgcca
     7201 gttcagcgtc gccgcgcact acgcgcatac cgcgaccacc gccgccgccg gaggctttga
     7261 taatcaccgg ataaccaatg cgtttagcaa tggcacggtt tttatccata tcgtcgccca
     7321 acgggccgtc agaacccggt acgcaaggga cgcccgcttt tttcatcgcc gcgattgcgg
     7381 atactttgtc gcccatcagg cgaatggttt ctgctttcgg gccgatgaag ataaagccgg
     7441 agcgttcaac ctgctcggca aagttggcgt tctcggagag gaagccgtaa cccggatgga
     7501 ttgctactgc gccggtgatt tcagcggcgc tgatgattgc cgggatgttc agataacttt
     7561 ttactgacgg agcagggcca atacagaccg tttcatctgc cagtaatacg tgttttagat
     7621 cgcgatccgc gctggagtgc acagcgacag tcttgatgcc cagttcttta caggcacgaa
     7681 gaatacgcaa tgcaatctcg ccgcggttgg caataacaat tttatccagc atgttcgcct
     7741 cgttactcga tgacgaccag cggctcgtca aattctaccg gttgtccact ttcgaccaga
     7801 attgctttca cggtaccgga tttgtccgct tcgatctggt tcatcatttt catggcttca
     7861 acgatgcaca gggtatcgcc cacgttgact ttctgaccca cttcgatgaa cgcttttgcg
     7921 tccgggcttg gggtgcggta gaaagtacca accatcgggg aacgtacgat gtgaccactg
     7981 atttccgctg ctgctggcgc ttccatggaa ggaacggtcg ccggagcggc tgcgttagat
     8041 tgagctggct gctgcatcat tggtgcagcg taagcttgtt gcatcacagg gaaacttgcg
     8101 gcaggagctg cacggctaat gcgtactgac tcttcgcctt cagaaatttc cagttcggag
     8161 atgcctgatt cttcaaccag ctcgatcagt tttttaatct tacgaatatc catgagtggg
     8221 ttccgtactc tttgtttagt gtgattgtga caggcgtttc accgccgtct gtaaagcgta
     8281 tgccccgaca gctgtatgca tagcgataaa ttccagcagg ccgggacgcc ggtctatttt
     8341 gccccagagt atcaatgtta gacttgacgg acattgtgca gcgatctgct gctatcctcc
     8401 ggcaaaaaac aaaatatacc ttcgttgcgc caatgtcacc ttttccgcag cgcaaaaact
     8461 gcgtcaggga ggacgaggtc gcacattata acgatttcgt agcaattggc agctaaatac
     8521 tggtctcatc agggaagata atcaacagct aacatgtaaa taaccttcaa caccgtgtaa
     8581 tttgcaacaa gccgcacaat tcacgaaatt agcgccatca tcgacggaac ttcttataac
     8641 gtaaggtgaa aagctgtaaa gccagccctg cgaagatgag gggctgcaat gagataatct
     8701 tcacagacca caaataatgt agggatgcca ggatcgcgac aagatagacg acattacgca
     8761 gcggttgctg gggattaccc aattttcact gcatcgtctg gatagatgtg aatgctaaag
     8821 cacgcaaaat caaccgactg acaattctca gcattaaata ggttcgagtg gttaatttgt
     8881 agtcaaataa aatcagatta ttcaccccat ggtaccagaa atcagtaaca ggttaagcgt
     8941 agtgtcgccc aggcaaagca ccataaacct aacaggcgac ggaggcgtat caataatggc
     9001 tgtttagagg agtatcaatg cgcatcaatt tgtggtcagt cgaaatcatg actttccagt
     9061 ttggatgatg ttgtacaact tcctgaccaa ggtgttcgac gaaagctgtc cggctaaccg
     9121 cttttcccgt taaccgattg aataatgatg tcaaaatgag ttccctctct tattattcct
     9181 tgctaaatgg ttagttaacc ttcaccagcg tgcgaccctg gatctggtta ttaatgatgg
     9241 cctcggcaaa gttcggtgcc tctgacagag atatctcttt tgccgcctgg gtatagaatg
     9301 attccggtaa atcggcgacc agtcgctgcc aggcttgtgc gcggcgttct ggtggcgtca
     9361 ttactgaatc caccccttgc aaacggacat tacgcagaat aaatggcatg accgtggttg
     9421 gcagagtaaa accacccgcc agaccacagg ccgccacgca gccgccgtaa ttcatttgcg
     9481 ccagcacttt tgccagcact ttgtcgccaa cagtgtcaat tgccccagcc cagacttgtt
     9541 tttccagagg acgggattcg gcaaactcat cacgagggag aatacggctg gcacctaaac
     9601 ttttcagata ttcatgggta ctttcgcgac cggaaacggc aacgacctga taacccaact
     9661 tatgcagcag cgccacggcg gtactgccga cgccaccact ggcacccgtc acgacaatct
     9721 ccccgtcctg cgggcgaaca ccggcatctt ccagcgccat cacacacagc atggcggtaa
     9781 aaccggcagt accgataatc attgctttac gcgcgtccag cccttgcggc atggcaacca
     9841 gccagtcacc tttcactcgc gcctgctccg ccagcccacc ccagtggttt tcaccaacgc
     9901 cccagccagt gagtaacacc tcctgaccgg catgaaaacg cggatcttcg ctggtgcgta
     9961 cagttccggc aaaatcgatc ccaggaatca tcggaaaatt acggatgatt tttcccttac
    10021 cggtaatcgc cagcgcatct ttatagttca ggctcgacca gtgaacatcg accgtgacat
    10081 cgccctccgg caggcgactt tcgtccagag tctgtactga tgcgagagtt ttgccgtcct
    10141 gctgttctaa aagtaacgcc tgcataagtg gtcctcatgt gcatgatgga ttggaaaata
    10201 atttctgaag attatactcg ctaatggaaa agcaaagccg atgaagcgca ataaattgcg
    10261 agataaatct gatttgctag tatgcccgct tcctcactat cggagttaac acaaggatga
    10321 gattaacgac gaaattttcg gcctttgtta cgctgctcac cgggttaaca atttttgtga
    10381 ctttgctggg ctgttcgcta agtttctaca acgcaattca gtataagttt agtcatcgcg
    10441 ttcaggcggt ggcgacggcg attgataccc accttgtgtc gaatgacttc agcacattaa
    10501 ggccacaaat taccgaatta atgatgtcgg cagatatcgt tcgtgtagac ctgctccatg
    10561 gtgataaaca ggtttatacc ctggccagaa atggtagtta tcgtccggtt ggctccagcg
    10621 atctgtttcg cgaactgagt gttccgttga taaagcatcc ggggatgtcg ctgcgtctgg
    10681 tttatcagga tccgatgggc aactatttcc attctttaat gactaccgcg ccgctcacgg
    10741 gggcgattgg ctttatcatt gttatgctct tcctggcggt acgctggtta caacggcaac
    10801 ttgccgggca agaattgctg gaaacccggg ctactcgtat cttaaacggt gagcgtggct
    10861 ctaatgtgtt gggaaccatc tatgaatggc cgcccagaac cagcagtgcg ctagatacgc
    10921 tgcttcgtga aattcagaac gcacgcgaac aacacagccg tcttgatacg ctgatccgct
    10981 cttatgccgc ccaggacgtg aaaaccggcc tcaataaccg actctttttc gataatcagt
    11041 tagcaacgtt actggaagat caggagaaag taggtaccca cgggatcgtg atgatgattc
    11101 gtctgccgga tttcaatatg ttgagcgata cttgggggca cagccaggtt gaagaacagt
    11161 tcttcactct gactaatctg ctgtcgacat ttatgatgcg ctaccctggc gcactgctgg
    11221 cgcgttacca ccgcagtgat tttgctgcgc tgttaccgca ccggacgtta aaagaggcag
    11281 agagcatcgc cggtcagtta atcaaagccg tcgatacctt gccgaacaat aaaatgctcg
    11341 atcgcgacga tatgatccac attggtatct gcgcctggcg tagtggtcag gataccgagc
    11401 aggtaatgga acatgcagag tctgccacgc gtaatgcggg attgcagggc ggcaatagct
    11461 gggctattta cgatgactcg ttgcctgaaa aaggacgcgg taatgttcgc tggcgtacgc
    11521 ttatcgagca aatgctcagt cgcggcggcc cgcgccttta tcaaaaaccg gcggttactc
    11581 gcgaaggtca ggttcatcat cgcgaactca tgtgccgcat cttcgatggt aatgaagagg
    11641 ttagctcggc ggagtatatg ccgatggtct tgcagtttgg cttatcggaa gagtatgacc
    11701 gtctgcaaat cagccgtctt attccactat tgcgttactg gccagaggaa aatctggcga
    11761 ttcaggttac cgttgagtcg ctgattcgcc cgcgttttca gcgttggctg cgcgatacgt
    11821 taatgcaatg tgaaaaatca caacgaaaac gcataattat tgaacttgca gaggccgatg
    11881 taggtcaaca tatcagtcgt ttacaacctg ttattcgttt agtgaatgct ttaggggtac
    11941 gggtagccgt caaccaggct ggtttgacgc tggtaagcac cagttggatc aaagaactta
    12001 atgttgagtt actcaagctc catccggggc tggtcagaaa cattgagaag cgaacggaga
    12061 accagctgct ggttcaaagc ctggtggaag cctgctccgg gaccagcacc cacgtttacg
    12121 ccaccggcgt gcgttcgcga agcgagtggc agaccctgat tcagcgcggt gttacaggcg
    12181 ggcaagggga ttttttcgcg tcctcacagc cacttgatac taacgtgaaa aaatattcac
    12241 aaagatactc ggtttaacct gccgtttaat ccgttttcac gtagaataac gcgcgctgcg
    12301 tctcatggga gtgtgcttgt ctgctcgcca gattgttgca gcacatatgc agatgaatga
    12361 ccttacgcgg ttgcaaacag gcgaggaatg ctgctgatgc attaagcctt tctggactca
    12421 ggcagagatt tgtaacaaag gaaacgaact gcactaattt tcaccgtagc agatgatttt
    12481 tgcgccttgt cgctgctgcg tgtggttggt aaagtaagcg gattttcttt tccgccccag
    12541 ctttcaggat tatcccttag tatgttgaaa aaatttcgtg gcatgttttc caatgacttg
    12601 tccattgacc tgggtactgc gaataccctc atttatgtaa aaggacaagg catcgtattg
    12661 aatgagcctt ccgtggtggc cattcgtcag gatcgtgccg gttcaccgaa aagcgtagct
    12721 gcagtaggtc atgacgcgaa gcagatgctg ggccgtacgc cgggcaatat tgctgccatt
    12781 cgcccaatga aagacggcgt tatcgccgac ttcttcgtga ctgaaaaaat gctccagcac
    12841 ttcatcaaac aagtgcacag caacagcttt atgcgtccaa gcccgcgcgt tctggtttgt
    12901 gtaccggttg gcgcgaccca ggttgaacgc cgcgcaattc gtgaatccgc gcagggcgct
    12961 ggtgcccgtg aagtcttcct gattgaagaa ccgatggctg ccgcaattgg tgctggcctg
    13021 ccggtttctg aagcgaccgg ttctatggtg gttgatatcg gtggtggtac cactgaagtt
    13081 gctgttatct ccttgaacgg cgtggtttac tcctcttccg tgcgcattgg tggtgatcgt
    13141 ttcgacgaag ctatcatcaa ctatgtgcgt cgtaattacg gttctctgat cggtgaagcc
    13201 accgcagaac gtatcaagca cgaaatcggt tcggcttacc cgggcgatga agtccgtgaa
    13261 atcgaagttc gtggccgtaa cctggcagaa ggtgttccac gcggttttac cctgaactcc
    13321 aacgaaatcc tcgaagcact gcaggaaccg ctgaccggta ttgtgagcgc ggtaatggtt
    13381 gcactggaac agtgcccgcc ggaactggct tccgacatct ccgagcgcgg catggtgctc
    13441 accggtggtg gcgcactgct gcgtaacctt gaccgtttgt taatggaaga aaccggcatt
    13501 ccagtagttg ttgctgaaga cccgctgacc tgtgtggcgc gcggtggcgg caaagcgctg
    13561 gaaatgatcg acatgcacgg cggcgacctg ttcagcgaag agtaatcgga tgcaggcagg
    13621 ggaaatgtct gtttaccctg cctggtctga tacgagaata cgcataactt atgaagccaa
    13681 tttttagccg tggcccgtcg ctacagattc gccttattct ggcggtgctg gtggcgctcg
    13741 gcattattat tgccgacagc cgcctgggga cgttcagtca aatccgtact tatatggata
    13801 ccgccgtcag tcctttctac tttgtttcca atgctcctcg tgaattgctg gatggcgtat
    13861 cgcagacgct ggcctcgcgt gaccaactag aacttgaaaa ccgggcgtta cgtcaggaac
    13921 tgttgctgaa aaacagtgaa ctgctgatgc ttggacaata caaacaggag aacgcgcgtc
    13981 tgcgcgagct gctgggttcc ccgctgcgtc aggatgagca gaaaatggtg actcaggtta
    14041 tctccacggt taacgatcct tatagcgatc aagttgttat cgataaaggt agcgttaatg
    14101 gcgtttatga aggccagccg gtcatcagcg acaaaggtgt tgttggtcag gtggtggccg
    14161 tcgctaaact gaccagtcgc gtgctgctga tttgtgatgc gacccacgcg ctgccaatcc
    14221 aggtgctgcg caacgatatc cgcgtaattg cagccggtaa cggttgtacg gatgatttgc
    14281 agcttgagca tctgccagcg aatacggata ttcgtgttgg tgatgtgctg gtgacctccg
    14341 gtctgggcgg tcgtttcccg gaaggctatc cggtcgcggt tgtctcttcc gtaaaactcg
    14401 atacccagcg cgcttatact gtgattcagg cgcgtccgac tgcaggactg caacgtttgc
    14461 gttatctgct gctgctgtgg ggcgcggatc gtaacggcgc taacccgatg acgccggaag
    14521 aggtgcatcg tgttgctaat gaacgtctga tgcagatgat gccgcaggta ttgccttcgc
    14581 cagacgcgat ggggccaaag ttacctgaac cggcaacggg gatcgctcag ccgactccgc
    14641 agcaaccgac gacaggaaat gcagctactg cgcctgctgc gcccacacag cctgctgcta
    14701 atcgctctcc acaaagggct acgccgccgc aaagtggtgc tcaaccgcct gcgcgtgcgc
    14761 cgggagggca atagtggcga gctatcgtag ccagggacgt tgggtaatct ggctctcttt
    14821 cctcattgcg ctgttgctgc aaatcatgcc ctggccggat aacctgattg ttttccggcc
    14881 aaactgggtg ttactcatct tgttgtattg gatcctggcc ttgcctcatc gcgtaaatgt
    14941 gggcacaggt tttgtgatgg gtgccatact ggatctgatc agcggctcga cgcttggcgt
    15001 acgcgtattg gcgatgagca tcattgctta cctggtggcg ctgaaatacc agcttttccg
    15061 caacctcgca ttatggcagc aggcgctggt cgtcatgttg ctttcgctgg tggtggatat
    15121 tattgttttc tgggcagagt ttttagtgat taacgtctct ttcagaccgg aagtgttctg
    15181 gagtagtgta gtcaatgggg tgctctggcc gtggattttc ttgctgatgc gtaaagtccg
    15241 tcagcagttt gcagtgcaat aaaggtttct atgacttctc tgtatttagc ttccggttct
    15301 ccgcgtcgtc aggagttact tgcgcaactt ggtgtgacct ttgaacgtat tgttacgggc
    15361 attgaggagc agcgtcagcc gcaggagagc gcgcagcagt atgttgtgcg tctggcgcgc
    15421 gagaaagcac aggcgggtgt cgcgcaaacg gcgcaggatc tcccggtgct gggtgcggat
    15481 actatcgtta tcctgaatgg agaagtgctg gagaaaccgc gcgacgcaga gcatgcggcg
    15541 cagatgttgc gcaaattatc gggtcagacc catcaggtga tgacggcagt ggcgttggcc
    15601 gacagccagc acattctcga ttgcctggtg gtcaccgatg tgactttcag aacgttaaca
    15661 gacgaagaca tcgcgggcta tgtcgccagc ggtgaaccgt tagataaagc aggtgcatac
    15721 ggtattcagg ggctgggtgg ctgttttgtc aggaagataa atggcagcta tcacgccgta
    15781 gtcggcttac ctctggttga aacgtatgaa ttattaagta attttaacgc actgcgtgag
    15841 aaaagggata aacatgacgg ctgaattgtt agtaaacgta acgccttcgg aaacgcgagt
    15901 ggcgtatatt gatggcggta ttctgcagga aattcatatt gaacgtgagg cgcgacgcgg
    15961 aatagtaggc aatatctaca agggtcgtgt aagtcgtgta cttccgggta tgcaggcggc
    16021 ttttgtagat attgggctgg ataaagccgc gtttcttcat gcatccgata tcatgccgca
    16081 caccgaatgt gtggcgggtg aagaacaaaa gcaattcacg gtgcgcgaca tctcggaact
    16141 ggttcgtcag gggcaagatc tgatggtgca ggtggtgaaa gatccgcttg gcactaaagg
    16201 tgcgcgcctg accaccgata tcacgctgcc ttctcgctat ctggtcttta tgccaggggc
    16261 ttctcacgtt ggggtttccc aacgtattga aagcgaatca gaacgtgaac gcctgaaaaa
    16321 agtggtcgca gagtattgcg acgagcaggg cgggtttatc atccgtaccg cagcggaagg
    16381 ggttggcgag gctgaactgg cctccgatgc cgcttatctg aaacgcgtct ggaccaaagt
    16441 tatggagcgt aaaaagcgcc cgcagacccg ttatcagctg tacggcgaac tggcgctggc
    16501 tcagcgtgtt ctgcgtgatt tcgccgacgc cgaactggac cgcattcgcg ttgactcacg
    16561 cctgacttac gaagcattac ttgagttcac ctcggagtac attcccgaga tgaccagcaa
    16621 gctggagcat tacaccggac gccagccgat tttcgatctc tttgatgtcg aaaacgaaat
    16681 ccagcgagcg ctggaacgca aagtagaact gaaatccggt ggttatctca ttatcgacca
    16741 gaccgaagcg atgaccaccg tggacatcaa taccggagcg tttgtcggtc atcgcaatct
    16801 ggacgacacc attttcaata ccaatattga agcgacgcag gctatcgctc gccagttacg
    16861 gttgcgtaat ctgggcggga ttatcattat tgatttcatc gatatgaata atgaagatca
    16921 ccgccgccgc gtgctgcact cgctggagca ggcgttgagc aaagaccggg tgaaaaccag
    16981 cgttaatggt ttttcggcgc tggggctggt ggagatgacg cgtaaacgca cccgcgaaag
    17041 cattgagcac gtactgtgta acgaatgccc aacctgccac ggtcgcggaa cggtgaaaac
    17101 cgtggaaacg gtatgctatg aaatcatgcg cgagattgtt cgtgtccacc atgcttacga
    17161 ctccgaccgt ttcctggtct atgcttctcc ggcagtagcc gaagccttga aaggcgaaga
    17221 gtcacactcg ctggcggaag tggaaatttt cgttggcaaa caggttaaag tacaaattga
    17281 accgctctat aaccaggagc agtttgacgt cgtaatgatg taaacagatg ctgggccgcc
    17341 atccggcaaa gggtttttga gtcacatttt tagcagacaa ggagtgacgg gtgaggcgat
    17401 tgccggggat tttactgctt actggagccg cgctcgttgt gatcgctgcc ctgctggtta
    17461 gcggcctgcg tattgcttta ccgcatcttg acgcctggcg tccggaaatc ctcaacaaaa
    17521 tagaatccgc gactggcatg ccggtagaag ccagtcagct ctcagccagc tggcagaatt
    17581 ttggcccgac gcttgaagca cacgacatcc gtgcagaact aaaagatggc ggcgaatttt
    17641 cggttaaacg cgttactctg gcgctggatg tctggcagag cctgttacat atgcgctggc
    17701 agtttcgcga cctcactttc tggcagctgc gctttcgcac caacactcct atcaccagcg
    17761 gtggtagcga tgacagtctg gaagccagtc acatcagcga tctgtttctt cgtcaatttg
    17821 accatttcga tctccgcgac agtgaagtca gtttcctgac gccatccggt cagcgtgccg
    17881 agctggcgat cccacaactc acctggctga acgatccacg tcgacaccgt gcggaaggcc
    17941 tggtaagcct ctccagcctt accggacagc acggcgtgat gcaggtgcgc atggatttgc
    18001 gcgatgatga ggggttgtta agcaatggtc gcgtctggct acaggcggat gacatcgacc
    18061 tgaagccgtg gctcggtaaa tggatgcagg acaatattgc tctggaaacg gcacagttct
    18121 cccttgaagg ctggatgacg atcgacaaag gcgatgtaac cggcggtgac gtctggctga
    18181 aacagggcgg tgccagctgg ttgggcgaga agcaaacgca tacgctgtcg gtggataatc
    18241 tgaccgcgca tattacgcgt gaaaatccgg gctggcagtt ctctattccc gatacacgga
    18301 tcacgatgga cggcaaaccc tggccgagcg gagcattgac gctggcctgg ataccggaac
    18361 aggacgttgg cggcaaagac aataaacgca gtgacgaact ccggattcgc gccagtaatc
    18421 tggagctggc aggcctggag ggcatacgcc cgctggccgc gaaactttca cctgcactgg
    18481 gtgatgtttg gcgctccaca caaccgagcg gcaagattaa cactctggcg ctggatatcc
    18541 cgcttcaggc ggcagacaag acccgttttc aggcatcgtg gagcgatctg gcctggaagc
    18601 aatggaaatt attaccgggt gcggaacact tctccgggac gctttccggc agcgttgaaa
    18661 atggtttgct taccgcgtcg atgaagcagg caaagatgcc ttacgaaacg gtattccgtg
    18721 cgccactgga aatcgccgac ggccaggcaa ctataagctg gctgaacaat gacaaaggtt
    18781 tccagctgga tgggcgtaat attgacgtta aagccaaagc cgtccatgcg cgcggcggtt
    18841 ttcgttacct gcaacctgct aacgatgaac cctggctggg tattctggct ggcatcagta
    18901 ccgatgatgg ttcacaagcc tggcactatt tcccggaaaa cttgatgggt aaagacctgg
    18961 ttgattactt aagtggcgcg attcagggcg gtgaagcgga taacgcgacg ctggtttatg
    19021 gtggcaatcc gcaactcttc ccctataaac acaacgaagg tcagtttgaa gtgctggtgc
    19081 cgctgcgcaa cgcgaagttt gccttccagc cggactggcc tgcattaact aaccttgata
    19141 ttgaactgga ctttattaac gacggtttat ggatgaaaac cgatggcgtt aatctgggcg
    19201 gcgtgcgcgc gagtaatctc accgcagtga tccctgacta ctcgaaagaa aaactgctga
    19261 ttgacgctga cattaaaggt ccgggtaaag ccgttggccc ttactttgat gagacaccgc
    19321 tgaaagattc tctgggtgcg accctgcaag aactccagct cgacggcgat gtgaatgctc
    19381 gcttacatct tgatatcccg ctgaacggcg agctggtaac cgcgaaaggt gaagtgacgc
    19441 tgcgtaataa cagtctgttt atcaaaccac tcgacagcac cctgaaaaat ttgagcggta
    19501 aattcagctt tatcaatggc gatctgcaaa gtgaaccact gacagcaagc tggtttaatc
    19561 agccgttgaa tgtggatttt tccaccaaag aaggggcaaa agcctaccag gttgcggtga
    19621 atctcaacgg taactggcaa ccggcgaaaa ccggcgttct gcctgaagcg gtgaacgaag
    19681 cattgagtgg cagcgtggcg tgggatggta aagtgggcat tgatctgcct tatcatgctg
    19741 gcgcgaccta taacgtagag ctgaacggcg atctgaataa tgtgagtagt cacttacctt
    19801 caccgttagc caaacctgcg ggtgaaccac tagcggtaaa cgttaaggtt gatggcaatc
    19861 tcaacagctt tgaattaacc ggacaggctg gtgcggataa ccatttcaat agccgctggt
    19921 tgcttggtca aaagctgacg ctcgatcgtg ctatttgggc ggcagacagt aaaacgctcc
    19981 cgccgttgcc ggaacaaagt ggcgttgaac tcaatatgcc gccgatgaat ggtgccgagt
    20041 ggctggccct gttccagaaa ggcgctgcgg agagtgtcgg tggtgcagcg agtttcccac
    20101 aatacataac gttacgtacg cctatgttgt cactgggaaa tcagcaatgg aataacctga
    20161 gtattgtttc gcaaccgacg gcaaatggca ccctggttga ggcgcaaggg cgtgaaatca
    20221 acgccacgct agcgatgcgt aataacgcgc cgtggctggc gaatatcaaa tatctttatt
    20281 acaacccgag cgtggcgaaa actcgtggtg attcaacgcc gtcatcacct ttcccgacaa
    20341 cggagcgcat taacttccgt ggctggccgg acgcacaaat acgatgcaca gagtgctggt
    20401 tctgggggca aaaattcggt cgcattgaca gtgatatcac catttctggc gatacgttaa
    20461 cgctgaccaa tggactgatt gatactggtt tctcgcggct tactgccgat ggtgaatggg
    20521 ttaataatcc ggggaatgaa cgtacctcgc tgaaaggaaa actgcgcggg cagaaaattg
    20581 atgccgccgc agaatttttt ggtgtcacga cgcccatacg ccagtcgtca tttaatgtgg
    20641 attacgattt acactggcgc aaagcaccct ggcagccaga tgaagcgacg ttgaatggca
    20701 tcattcatac tcaactgggt aaaggcgaaa ttaccgaaat caataccgga catgccgggc
    20761 aattgctgcg cttattgagc gtagatgccc tgatgcgtaa gctgcgtttt gatttcagag
    20821 acacttttgg cgaagggttc tattttgact ccattcgcag caccgcgtgg attaaagacg
    20881 gggttatgca caccgacgac acgctggtgg atggcctgga ggcggatatc gccatgaaag
    20941 ggtcggtaaa tctggtacgt cgcgacctga atatggaagc ggttgtcgca ccagagattt
    21001 ctgcgacggt gggcgtggct gcggcttttg cggttaaccc cattgttggc gcggcagttt
    21061 ttgccgctag taaagtgctg gggccgctgt ggagcaaagt ctccattttg cgctatcaca
    21121 tttcgggtcc gctggacgat ccgcaaatca acgaagtgtt gcgccaaccg cgtaaagaaa
    21181 aagcgcaatg atttgacgag ggcgcgtaat tgccccaatc tcatatgata atcgttgcca
    21241 aaggccaacg agccagaaca taaccgtagg ccggataggg cgttcatgcc gcatccggca
    21301 gccgtaaaaa atcctctact gcagtaacta acgagtagca aaaacgatga gtctgaacct
    21361 ggtaagtgaa caattgctag cggcgaacag cctgaaacat caggacttgt tcgcgatcct
    21421 cggtcaactg gccgaacgtc gccttgatta tggcgatctc tattttcagt cgagctatca
    21481 cgaatcctgg gttttagaag accgcattat taaagatggt tcttacaaca tcgatcaggg
    21541 cgttggcgtg cgtgcaatca gcggtgaaaa aaccggattt gcttacgctg accaaatcag
    21601 cctgctggcg ctggaacaga gtgcgcaagc ggcgcgcacc atcgtccgtg atagtggtga
    21661 tggcaaagta cagacgctgg gcgcggtaga gcatagcccg ttgtatacct cggtagatcc
    21721 gctgcaaagc atgagccgtg aagagaagct ggatatcctg cgtcgcgtcg ataaggttgc
    21781 tcgcgaagcg gacaagcgcg tacaggaagt gactgccagc ctcagtggtg tctatgaatt
    21841 aattctggtt gcggccaccg acggcacgct agcggcggat gtccgtccgc tggtgcgtct
    21901 ttccgtgagc gttctggtcg aagaagatgg caaacgcgaa cgcggtgcca gtggcggcgg
    21961 cggtcgtttt ggttatgagt tcttccttgc cgatcttgac ggcgaagtcc gtgcggatgc
    22021 atgggcaaaa gaagcagtgc gtatggcgct ggtcaatctt tctgccgttg ctgcaccagc
    22081 gggcaccatg ccggtagtac ttggcgcagg ttggccgggc gtgctgttgc atgaagcggt
    22141 aggtcacggt ctggaaggcg acttcaaccg ccgcggcact tcagtattta gtgggcaggt
    22201 cggggagctg gtggcttcag aactgtgtac cgtggttgat gacggcacga tggtcgatcg
    22261 ccgtggttcg gtggcgattg atgacgaagg tacgccaggc cagtacaacg tgctgattga
    22321 gaacggcatt ctgaaaggct acatgcagga taaactcaac gcgcgtttga tggggatgac
    22381 gccgactggc aacggtcgcc gtgaatccta cgcccatctg cccatgccgc gtatgactaa
    22441 cacctatatg ctgccgggta aatcgacccc gcaggaaatt attgaatccg ttgagtacgg
    22501 tatctatgca ccgaactttg gtggcggtca ggtggatatc acctccggca aattcgtttt
    22561 ctccacttca gaagcatatc tgattgaaaa cggtaaagta acgaagccgg tgaaaggcgc
    22621 aacgttgatt ggttccggta tcgaaaccat gcagcagatt tcgatggttg gcaacgacct
    22681 gaaactggat aacggcgtgg gtgtctgcgg taaagaaggg caaagtttgc cggttggcgt
    22741 gggccagcca acgctgaaag ttgataacct gactgttggc ggtactgcgt aataatttca
    22801 ttattttcag aaggataatt aatctttcta cgtgcacgaa cggtcccctc gctcctctgg
    22861 ggttagggtg aggggaaccc gttggcacag gtttgtagaa cgtaacagta caatatgaat
    22921 tacttctctt tcccgcgccc gtgcatctcc tgaaacaatt taccgacctc aacaaaataa
    22981 tccgtcagcg agttgatcac gacctgtacc ttcagcggca gcttatcttt ttcggtatat
    23041 aacgcataaa ccgggcgtgg atctgactga taacgcggca gcaggatctc cagctcaccg
    23101 cgattgatct cgttgatcac ccacatcagc ggcacgtagg cgatcccggc acccgccgtc
    23161 agccagcgca ccagcgtcat cggatcatta gtcacaaatc ttccttgtgg gatcaggcga
    23221 gtcgagatcc cttccggtgc gatcagttca aattcattgt cgggccgcac gctgtattca
    23281 agccatgaat gactactcaa atcggcgggt ttttccggta tgccgtattg tgtgagatag
    23341 cttttcgcgg cgcacaccac catcggcatc gcgcccagac ggcgggaaaa caggctggaa
    23401 tcctgcaacg cgccgacgcg gatcaccaca tccagaccgt cggcaatcag gtcgggggct
    23461 ggaattccgg taaccagatt gacgctcaaa cctgggtatt ctttcagcat ttttgctgtc
    23521 agcccggcga gaacattttg tgccatagtt gaagaacagc caatgcgcag cgtcccgatg
    23581 ggggtgttat tgaaggcata cagttgctca tgaacatcct gcacttcatg aagcatccga
    23641 cggcagccct ggtagtaaat tctgccggct tcggtcaggc caatgctgcg tgtgctacgg
    23701 tttaacagct ttacctgcaa ctcatcttcc agttttgaca ccgtctgact gatggacgaa
    23761 acgctcatct gtagctgtct ggcggcggcg gtaaaagagc caaattcaac tactttggca
    23821 aacaccgaca tgcgttttag tcgttccatt attcactctg acttaaaagt gatttagatc
    23881 acataatata gataacagca taacagttac gctaatatat taaatatcaa tctacagcaa
    23941 tgttgctctc gcccggcttt ccatgccctt ctctgcggcg acagatgctg aaaataataa
    24001 cgcctgctct ctctttacaa ccaaggtcaa catgagtctg tttcccgtta tcgtggtgtt
    24061 tggactgtcc ttcccaccga tattttttga attgctttta tcactggcga ttttctggct
    24121 ggtgcgccgg gtacttgtgc caacaggtat ctacgacttt gtctggcatc cggcgttgtt
    24181 caacaccgcg ctctattgct gcttgtttta tttgatatcg cgactgttcg tttgaggttg
    24241 aagtgaaaac actaataaga aaattctccc gtacggccat cacggtcgta ttagtcattc
    24301 tagccttcat cgcaattttt aatgcctggg tctattacac cgaatccccc tggacgcgtg
    24361 acgcgcgctt tagcgctgac gtcgttgcga tcgcgccgga cgtttctgga ctcattaccc
    24421 aggtgaatgt tcatgataac cagctggtga aaaaaggaca gatactgttc accatcgacc
    24481 agccgcgcta tcaaaaggcg cttgaggaag cgcaagccga tgttgcttat tatcaggtac
    24541 tggcacagga gaaacgccag gaggccggac gtcgtaaccg tctcggtgtg caggcgatgt
    24601 ctcgcgaaga gatcgaccag gccaacaacg tactacaaac ggttctgcat cagttagcga
    24661 aagcgcaggc gacccgcgat ctggcaaaac tggatcttga acgcacggtg atccgcgcgc
    24721 cagcagatgg ctgggtgacc aacctcaacg tctataccgg tgagtttatt actcgaggat
    24781 caacggcggt tgcgctggtg aaacagaact ccttctatgt actggcctat atggaagaaa
    24841 ctaagctgga aggggggcgt ccggggtatc gtgcagagat cacgccgctt ggcagtaaca
    24901 aagtgctgaa agggactgtt gatagtgttg ccgcaggggt caccaacgcc agcagcacgc
    24961 gtgacgacaa agggatggcg actatagact ctaatcttga atgggtgcgt cttgcgcaac
    25021 gtgttccggt tcgtattcgt ctcgacaacc agcaagagaa catctggcct gcgggcacca
    25081 ctgctacagt ggtggtcact ggcaaacaag atcgcgacga aagccaggat tcgttcttcc
    25141 gtaaaatggc ccatcgcctg cgtgagtttg gttaatcacg atgggtattt tctccattgc
    25201 taaccaacat attcgctttg cggtaaaact ggcgaccgcc attgtactgg cgctgtttgt
    25261 tggctttcac ttccagctgg aaacgccacg ctgggcggta ctgacagcgg cgattgttgc
    25321 cgccggtccg gcctttgctg cgggaggtga accgtattct ggcgctattc gctatcgtgg
    25381 ctttttgcgc atcatcggca catttattgg ctgtattgcc ggactggtga tcatcattgc
    25441 gatgatccgc gcaccattat tgatgattct ggtgtgctgt atctgggccg gtttttgtac
    25501 ctggatatcc tcgctggtac gaatagaaaa ctcgtacgcg tgggggctgg ccggttatac
    25561 cgcgctgatc attgtgatca ccattcagcc ggaaccattg cttacgccgc agtttgccgt
    25621 cgaacgttgt agcgagatcg ttatcggtat tgtgtgtgcg attatggcgg atttgctctt
    25681 ttctccgcga tcgatcaaac aagaagtaga tcgagagctg gaaagtttgc tggtcgcgca
    25741 atatcaatta atgcaactct gtatcaagca tggcgatggt gaagttgtcg ataaagcctg
    25801 gggcgacctg gtgcgacgca ccacggcact acaaggcatg cgcagcaacc tgaatatgga
    25861 atcttcccgc tgggcgcggg ccaatcgacg tttaaaagcg atcaatacgc tatcgctgac
    25921 gctgattacc caatcctgcg aaacttatct tattcagaat acgcgcccgg aattgatcac
    25981 tgatactttc cgcgaatttt ttgacacgcc ggtagaaacc gcgcaggacg tccacaagca
    26041 gctcaaacgc ctgcggagag ttatcgcctg gaccggggaa cgggaaacgc ctgtcaccat
    26101 ttatagctgg gtcgcggcgg caacgcgtta tcagcttctc aagcgcggcg ttatcagtaa
    26161 cacaaaaatc aacgccaccg aagaagagat cctgcaaggc gaaccggaag taaaagtaga
    26221 gtcagccgaa cgtcatcatg caatggttaa cttctggcga accacacttt cctgcattct
    26281 gggcacgctt ttctggctgt ggacgggctg gacttccggc agtggtgcaa tggtgatgat
    26341 tgcggtcgtg acgtcactgg caatgcgttt gccgaatcca cgcatggtgg cgatcgactt
    26401 tatctatggg acgctggccg cgctgccgtt agggctgctc tactttttgg tgattatccc
    26461 taatacccaa cagagcatgt tgctgctgtg tattagcctg gcagtgctgg gattcttcct
    26521 cggtatagaa gtacagaaac ggcgactggg ctcgatgggg gcactggcca gcaccataaa
    26581 tattatcgtg ctggataacc cgatgacttt ccatttcagt cagtttctcg acagcgcatt
    26641 agggcaaatc gtcggctgtg tgctcgcgtt caccgttatt ttgctggtgc gggataaatc
    26701 gcgcgacagg actggacgtg tactgcttaa tcagtttgtt tttgccgctg tttccgcgat
    26761 gactaccaat gtggcacgtc gtaaagagaa ccacctcccg gcactttatc agcagctgtt
    26821 tttgctgatg aataagttcc caggggattt gccgaaattt cgcctggcgc tgacgatgat
    26881 tatcgcgcac cagcgcctgc gtgatgcgcc gatcccggtt aacgaggatt tatcggcgtt
    26941 tcaccgacaa atgcgccgca cagcagacca tgtgatatct gcccgtagcg atgataaacg
    27001 tcgtcggtac tttggccagt tgctggaaga actggaaatc taccaggaaa agctacgcat
    27061 ctggcaagcg ccaccgcagg tgacggaacc ggttcatcgg ctggcgggga tgctccataa
    27121 gtatcaacat gcgttgaccg atagttaagt caaaaccgac gccaaaagcg tcggtttttt
    27181 catggctata cttagcgatg cacggcagaa ctcgccgcga aacgtgacgg tggcaacaga
    27241 tgaatattta tacctttgat tttgatgaga ttgagagtca ggaggatttt tatcgtgact
    27301 ttagccaaac ctttggtctg gctaaagata aggtacgcga tctcgactca ctatgggatg
    27361 tgttaatgaa cgatgtcctg ccgctaccac ttgagattga atttgttcat ctgggagaga
    27421 aaacgcgtcg ccgttttggc gcgttaatat tgctgtttga tgaggcagag gaagagctgg
    27481 aagggcattt gcgttttaat gttcgtcatt agcgcaaaaa aaagcccccg aaccgggggc
    27541 aatatcgtcg gacaagacga tgagggttta tttgtacagt tcagccgtag cgtgccaggt
    27601 gtcaccgcta cgagcttcag taatctggta ggccgttgcg cctttctctt ccgctttttt
    27661 gttcagcatt tcacgcatat ccattggcga agacgccaca ccacttacgg atacggtccc
    27721 gattgcttca cgattttgtg cttgtgcagt atcaatggag tcggcagcga atgcaccgaa
    27781 agagagaaca gaaagtacgc ttaatgcagc tacagtggtt ttgattttca tgatttttac
    27841 ctcgacataa tcttttagct gggcctttgt ttcgtgaccc atatcacaaa atcaagcata
    27901 cactaattat tgaactaatt aataccacgc taactgtttc agttgatact aagtgttaaa
    27961 aatgtgaatt ttaataacaa aaaagaatta aaaacacggt tatttattgt taattctagt
    28021 attttcaatt agataatgaa ttttacataa tgtgcttttt ttaccgtatg tattcattgt
    28081 gtgaatgata tgtcgcggta aaacgcacta ttcgttaaat taatcgcagg ggaaagcagg
    28141 gggttgagag gaataagcaa cattttcccc gccgccagaa gcgacggggc agagattaaa
    28201 gctcctggtc gaacagctct aaaatcgctt cgtacaggtc tttgacggtg aaaccgttag
    28261 caggggtggt aaagatggtg tcatcgccag cgatggtgcc cagaatacct tctgctttgc
    28321 ccagtgagtc cagcaggcga gcaattaact gcgccgcgcc agggctggta tgaatcacga
    28381 caactgcatc gttgtagtcg atatccagca ccagattctt caatggactg gaggtggttg
    28441 gtacacccag ttcagctggc aggcagtaaa ccatttccat tttggcattg cgtgtacgta
    28501 cagcaccaaa cttggtcaac atccgcgaga ctttagactg attaatattg tcaaagcctt
    28561 gctcctgcaa cgcggcgacg atttcgccct gggagctaaa tttctcttct ttaagtaatg
    28621 ctttaaatgc tttaactagt tcttcttgct tagccgagct tcgcataagt cacccgatat
    28681 ggtggttgat acaacattat tgtgcataca gatgaatttt tatgcaaaca gtcagccctg
    28741 aagaaggctg aaataatgtt atgaaagagc gggattttat caaatttcgt tattgagaaa
    28801 catgcctgcg tcacggcatg caaattctgt ttaaaagtaa attaattgtt atcaaattga
    28861 tgttgttttg gctgaacggt agggtatatt gtcaccacct gttggaatgt tgcgctaatg
    28921 cataagcgac tgttaattac gtaagttagg ttcctgatta cggcaattaa atgcataaac
    28981 gctaaacttg cgtgactaca cattcttgag atgtggtcat tgtaaacggc aattttgtgg
    29041 attaaggtcg cggcagcgga gcaacatatc ttagtttatc aatataataa ggagtttagg
    29101 atgaaagtcg cagtcctcgg cgctgctggc ggtattggcc aggcgcttgc actactgtta
    29161 aaaacccaac tgccttcagg ttcagaactc tctctgtatg atatcgctcc agtgactccc
    29221 ggtgtggctg tcgatctgag ccatatccct actgctgtga aaatcaaagg tttttctggt
    29281 gaagatgcga ctccggcgct ggaaggcgca gatgtcgttc ttatctctgc aggtgtagcg
    29341 cgtaaaccgg gtatggatcg ttccgacctg tttaacgtta acgccggcat cgtgaaaaac
    29401 ctggtacagc aagttgcgaa aacctgcccg aaagcgtgca ttggtattat cactaacccg
    29461 gttaacacca cagttgcgat tgctgctgaa gtgctgaaaa aagccggtgt ttatgacaaa
    29521 aacaaactgt tcggcgttac cacgctggac atcattcgtt ccaacacttt tgttgcggaa
    29581 ctgaaaggca aacagccagg cgaagttgaa gtgccggtta ttggcggtca ctctggtgtt
    29641 accattctgc cgctgctgtc acaggttcct ggcgttagtt ttaccgagca ggaagtggct
    29701 gatctgacca aacgcatcca gaacgcgggt actgaagtgg ttgaagcgaa agccggtggc
    29761 gggtctgcaa ccctgtctat gggccaggca gctgcacgtt ttggtctgtc tctggttcgt
    29821 gcactgcagg gcgaacaagg cgttgtcgaa tgtgcctacg ttgaaggcga tggtcagtac
    29881 gcccgtttct tctctcaacc gctgctgctg ggtaaaaacg gcgtggaaga gcgtaaatct
    29941 atcggtaccc tgagcgcatt tgaacagaac gcgctggaag gtatgctgga tacactcaag
    30001 aaagatatcg ccttgggcga agagttcgtt aataagtaat tgattagcga ataataaaaa
    30061 accggagcac agac
//
LOCUS       DAKISA010000045        30070 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.045, whole genome shotgun sequence.
ACCESSION   DAKISA010000045 DAKISA010000000
VERSION     DAKISA010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30070
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.045"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..312
                     /gene="ypdB"
                     /locus_tag="OVW45_002106"
     CDS             <1..312
                     /gene="ypdB"
                     /locus_tag="OVW45_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCV5526558.1"
                     /translation="VKDERIIVTPINDIYYAEAHEKMTFVYTRRESYVMPMNITEFCS
                     KLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYILRLKDLDFEVPVSRSKVKEFRQLMH
                     L"
     gene            325..1182
                     /locus_tag="OVW45_002107"
     CDS             325..1182
                     /locus_tag="OVW45_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCV5526559.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(1185..3680)
                     /gene="ptsP"
                     /locus_tag="OVW45_002108"
                     /pseudo
     CDS             complement(1185..3680)
                     /gene="ptsP"
                     /locus_tag="OVW45_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(3705..4742)
                     /gene="ypdE"
                     /locus_tag="OVW45_002109"
     CDS             complement(3705..4742)
                     /gene="ypdE"
                     /locus_tag="OVW45_002109"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCV5526560.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVSAEIGVPLQAD
                     MFSNGGTDGGAVHLTDTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(4742..5827)
                     /gene="ypdF"
                     /locus_tag="OVW45_002110"
                     /pseudo
     CDS             complement(4742..5827)
                     /gene="ypdF"
                     /locus_tag="OVW45_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(5842..7089)
                     /locus_tag="OVW45_002111"
     CDS             complement(5842..7089)
                     /locus_tag="OVW45_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCV5526561.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(7111..7437)
                     /locus_tag="OVW45_002112"
     CDS             complement(7111..7437)
                     /locus_tag="OVW45_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCV5526562.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(7657..8622)
                     /gene="glk"
                     /locus_tag="OVW45_002113"
     CDS             complement(7657..8622)
                     /gene="glk"
                     /locus_tag="OVW45_002113"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCV5526563.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALTLGTFGGVYIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            8826..10082
                     /locus_tag="OVW45_002114"
     CDS             8826..10082
                     /locus_tag="OVW45_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCV5526564.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPIWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            10197..10523
                     /locus_tag="OVW45_002115"
     CDS             10197..10523
                     /locus_tag="OVW45_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCV5526565.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(10664..11902)
                     /locus_tag="OVW45_002116"
     CDS             complement(10664..11902)
                     /locus_tag="OVW45_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCV5526566.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            12238..13440
                     /gene="nupC"
                     /locus_tag="OVW45_002117"
     CDS             12238..13440
                     /gene="nupC"
                     /locus_tag="OVW45_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCV5526567.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLRFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(13490..15678)
                     /locus_tag="OVW45_002118"
                     /pseudo
     CDS             complement(13490..15678)
                     /locus_tag="OVW45_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(15887..15962)
                     /locus_tag="OVW45_002119"
     tRNA            complement(15887..15962)
                     /locus_tag="OVW45_002119"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15927..15929),aa:Ala,seq:ggc)
     gene            complement(16002..16077)
                     /locus_tag="OVW45_002120"
     tRNA            complement(16002..16077)
                     /locus_tag="OVW45_002120"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16042..16044),aa:Ala,seq:ggc)
     gene            16298..16657
                     /locus_tag="OVW45_002121"
     CDS             16298..16657
                     /locus_tag="OVW45_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCV5526568.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            16659..17051
                     /locus_tag="OVW45_002122"
     CDS             16659..17051
                     /locus_tag="OVW45_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCV5526569.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQGKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(17103..18518)
                     /gene="gltX"
                     /locus_tag="OVW45_002123"
     CDS             complement(17103..18518)
                     /gene="gltX"
                     /locus_tag="OVW45_002123"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCV5526570.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            18777..18852
                     /locus_tag="OVW45_002124"
     tRNA            18777..18852
                     /locus_tag="OVW45_002124"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18810..18812,aa:Val,seq:tac)
     gene            18897..18972
                     /locus_tag="OVW45_002125"
     tRNA            18897..18972
                     /locus_tag="OVW45_002125"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18930..18932,aa:Val,seq:tac)
     gene            19019..19094
                     /locus_tag="OVW45_002126"
     tRNA            19019..19094
                     /locus_tag="OVW45_002126"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19052..19054,aa:Val,seq:tac)
     gene            19099..19174
                     /locus_tag="OVW45_002127"
     tRNA            19099..19174
                     /locus_tag="OVW45_002127"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19132..19134,aa:Lys,seq:ttt)
     gene            19294..19635
                     /locus_tag="OVW45_002128"
     CDS             19294..19635
                     /locus_tag="OVW45_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCV5526571.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASKIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIDIYI"
     gene            complement(19626..20552)
                     /locus_tag="OVW45_002129"
     CDS             complement(19626..20552)
                     /locus_tag="OVW45_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCV5526572.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGAPLYQI"
     gene            20642..21640
                     /locus_tag="OVW45_002130"
     CDS             20642..21640
                     /locus_tag="OVW45_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCV5526573.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(21637..21855)
                     /locus_tag="OVW45_002131"
     CDS             complement(21637..21855)
                     /locus_tag="OVW45_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCV5526574.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(21857..23872)
                     /gene="ligA"
                     /locus_tag="OVW45_002132"
     CDS             complement(21857..23872)
                     /gene="ligA"
                     /locus_tag="OVW45_002132"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCV5526575.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEVLEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(23943..24929)
                     /gene="zipA"
                     /locus_tag="OVW45_002133"
     CDS             complement(23943..24929)
                     /gene="zipA"
                     /locus_tag="OVW45_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCV5526576.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPIQQPPEAQVPPQHAPRPAQPMQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            25159..25920
                     /gene="cysZ"
                     /locus_tag="OVW45_002134"
     CDS             25159..25920
                     /gene="cysZ"
                     /locus_tag="OVW45_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCV5526577.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            26105..27076
                     /gene="cysK"
                     /locus_tag="OVW45_002135"
     CDS             26105..27076
                     /gene="cysK"
                     /locus_tag="OVW45_002135"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCV5526578.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            27460..27717
                     /gene="ptsH"
                     /locus_tag="OVW45_002136"
     CDS             27460..27717
                     /gene="ptsH"
                     /locus_tag="OVW45_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCV5526579.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            27762..29489
                     /gene="ptsI"
                     /locus_tag="OVW45_002137"
     CDS             27762..29489
                     /gene="ptsI"
                     /locus_tag="OVW45_002137"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCV5526580.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            29530..30039
                     /gene="crr"
                     /locus_tag="OVW45_002138"
     CDS             29530..30039
                     /gene="crr"
                     /locus_tag="OVW45_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCV5526581.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
BASE COUNT         7381 a         7961 c         7456 g         7272 t
ORIGIN      
        1 gtgaaagatg agcgaataat cgtcacgcca attaacgata tctattacgc cgaagcgcac
       61 gagaaaatga cctttgtcta tacgcggcgt gaatcctacg taatgccgat gaacattacc
      121 gaattttgca gcaaactgcc gccgtcgcat tttttccgct gccatcgctc attttgtgtc
      181 aatctgaaca aaatacgcga aatcgaaccg tggtttaata acacctacat tctgcgactg
      241 aaagatctgg attttgaagt gccggtcagc cgcagcaaag tgaaagaatt tcgccagtta
      301 atgcatcttt aatcgggaac tttcatgaaa gcaccaggct tgcccgccga tcaacaattt
      361 ttcgccgatc tgttcagcgg cctggtgctt aacccgcaac tactggggcg cgtctggttt
      421 gccagccagc ctgcctcgtt accggtgggc agtttatgta ttgattttcc ccgtctggat
      481 atcgtgctgc gcggcgaata cggcaacctg ctggaagcaa agcagcagcg aatggtggaa
      541 ggagaaatgc tgtttattcc ggcacgcgcg gctaatttac cgatcaacaa caaaccggtg
      601 atgctgttaa gcctggtgtt cgcaccgacc tggcttgggt tatcgtttta cgatagccgc
      661 accacgtcgt tgttgcatcc tgctcgccag atccagcttc ccagcctgca acgcggtgaa
      721 ggtgaagcga tgcttaccgc cctcacccat cttagccgtt cgccgctgga gcagaatatc
      781 attcagccac tggtgttaag tttgctgcat ctttgccgca acgtggtgaa tatgccgccg
      841 ggtaattcgc aaccgcgcgg cgattttctc tatcacagca tttgtaactg ggttcaggat
      901 aattatgccc agccgctcac ccgcgagagc gtggcgcagt tttttaatat cacgcccaat
      961 catctgtcaa aactgtttgc tcagcatgga acgatgcgtt ttatcgagta tgtgcgttgg
     1021 gtgcgaatgg cgaaggcgag gatgattttg cagaaatatc atctgtcaat tcatgaagtg
     1081 gcacagcgtt gcggttttcc ggatagcgac tatttttgtc gcgttttccg gcgtcagttt
     1141 ggtctgacgc cgggagagta cagcgcccgt tttcagggct aacgtcagaa ggttaattct
     1201 gtttccagca gcgtcaggat actttgtgca tcttgcgcgg caaacaacga ctggcggaag
     1261 tttttattca ccagtttacg cgccagctgc gagaagactt tcacatgatt catcccttcg
     1321 ttagcaccca gcgtcagcat gatcaccagt tcaacttcgc ccatttctga ctgccagtca
     1381 accggtttcg ccagccgggc aatgctgata ctggaatgac ggatccactg agatttggtg
     1441 tgcgggatcg ctacgccaaa accaacgccg gtggtaacaa tctcttcccg ctgccagaca
     1501 tcttcttcca gctcaaacgg atgttcagtg cgcccgttaa cgccgaggtt gccgcacagg
     1561 aactggatcg cctgctcttt attgctaaaa tcctgatcaa caaagatatt ctccagcgcc
     1621 agcagtgggc gaacgtcttc ttccggcgta aaggcggtga gtaacgcttc aatttcctgc
     1681 gcactgcggc attcacatgc ctgacgcgcc agttcccgac acgcctcgct atccagttga
     1741 cgaagctggc ttttcaccgc cggaatacgc gggctactca tactcagctc atccaggccc
     1801 agcccaagca gtagcggcag ataacggctt tcaccgccca gttcaccgca aatgcctacc
     1861 catttgcccc gctgatgcgc agtggtaact atttgctgca acatgcgcag gaacgatggc
     1921 gtaatcgggt tatatagcgg cgatacgcgc gggttattac gatcgaccgc atacagatac
     1981 tgggtcatat cgttggagcc gatactgaag aaatcgacct catcgcagaa gtggtcgatg
     2041 atgtagcaca ccgacggcac ttccaccatg atcccaagcg taatcgtctc tgcatgacgc
     2101 aggccatcgc gcttaagctc aacgatcgct ttttggatct cgcctttcac ccataagatc
     2161 tgatcgaggc tgtgaaccat cgggatcatc aactgggcgt tgccgaaact ggcggcgcgc
     2221 aaaatggccc gcagttgagt gcggaacagg ccagcaaatt ccgggtaaat acgtaccgcg
     2281 cgatagccga ggaacgggtt ctcttcctgg ggaatattca gataaggaat gcttttatcg
     2341 ccgccgatgt ccatcgtgcg gaagataatc ggcttgtcgc ccgccgccag tagcacctgc
     2401 tggtaggctt caaattgctc ctgctcgtcc ggcgcgctgt cgcgatccat atagagcatt
     2461 tcggtacgga acagcccgac accttccgcg ccgttggcaa acgcgcctgg cgcttccaga
     2521 gcggtgccga tattcgccgc aatatcaata cgcttgttat cacgggaata ggccagctgc
     2581 gcggcagcct gcgcctgttg tttttggcgt ttatccgcca gcgtctgcgc gacctgataa
     2641 taaccgctca ccgcgtcatt cgggttaatc gccagcacgc cgcactgggc gtcaagcact
     2701 gcaggttgcc cggcataacg ggcaatcgca tccagcggca agccactcag taccgggatc
     2761 gccgaggcac gggccagaat cagtgtatgc gaggtgcgcc cggttttctc cagaatcatg
     2821 cccgcaagat ttttcaaatc aaggctcaaa aactggcttg gggttaaatc ttcagccacc
     2881 agaatggtcg gtttttccag caccagattg ttgcgcggct tcagttccgg ctaggtgata
     2941 tgcagcaact gttcgctgat atcgcgaatg tcgctgaccc gctcgcgcag ataatcgctg
     3001 gcagaagcag atagtttggc gcaaacctgc tccatattgc tgatgatcgc cgcccccagt
     3061 ccctgatgct gttctgtcat caggcgacgg atattgcctg caaattcatc atcctgaatc
     3121 agcgacaaat gagcgctgag gatagttttg ctttcgccgt cacgctcacg caattgctgg
     3181 ttcagttgct cggcaagcgt tgccaggctg tgctccagcc gggtggaatc ttgcgcactg
     3241 gcggggattg cccgataact gtcgaggctg tcgctttgta acagggtcag cgtacccacg
     3301 ccgacgccgc ttgccagcac attgccgtac agtaaatccg ggttaaggcg gcttaatgaa
     3361 cgcggcagcg gatgcgccgt cagttctgcc tgcgtcggct gaacgctgtc gctgtcgata
     3421 aagcgcacca ggatgtactc ttccagcacg cgccgcgcct gctcttcatc gctgccgcta
     3481 atgttcaggc tgcaactgtc attaaataag gtgccagttc caatcaacgc cagcgagctt
     3541 ttagcatcgg cctttgcgtt ctggcgatgg ttgataaagg tgatttcgct ttgccactgg
     3601 ctgcactgtt ctttaagttc ccacgccgga cgagcatgta gaccgttagg caaaggacag
     3661 agaaattgaa tcgttaacat aaccgctccg ggttaatcag gagatcatct gaaatccgtc
     3721 agttgaacaa ccgtctcacg cgtaagacgt tgaataaggg cagataaaag ttgctccatc
     3781 tgcaaaatgt cgcggcaatc ggcaatcgat gccgcgcaat gtccgtggcg ggtggcaggc
     3841 cccattacca ctgtgggtac gccagtatcg gttaagtgca ccgctccacc gtccgtgccg
     3901 ccgttactga acatatccgc ctgcaacgga acgccaattt ctgccgacac ggtttcaatc
     3961 cacgcggtaa gttttggcgg cgcaatcagc gatttatcgc ttaacaccag catcggcccg
     4021 ttaccaatct gacgatggtt agccgcgcca taatcaaagt ttttcgccca gcaggcggta
     4081 tcaagcacaa tggcgacgtc cggcgacacc gcgcgggtag cagtttgccc gccgcgtaat
     4141 cccacctctt cgctggaact tgccaccagc cacacttccg caggcagttc ggcgtcgtgc
     4201 agctcacgta gtaacgtcac cagcagatag caaccgaggc ggtcatcaaa ggctttcccc
     4261 atcacccgct ggtgagggag aacctgaaaa gttgtatcaa aggtgacgcg atcgcctgga
     4321 cgaattcccg cctgcatcac ttcgtcatac gagcgcgcac caatgtccac gcgcatggcg
     4381 ctgacgtcat tcccctgccg gtcgccgtca agcaggcctg gaattttgca ctcttcacgg
     4441 gtggtgatgc gcaccggctg cagctggcgg gcagccatgc gcacgttgcc aaccggcagc
     4501 acatcaattg ccccttcgcg ggagatgctg cgcaccataa atcccacttc gtccatatgc
     4561 gcacagatca tcaccttcgg acctgtcgat tcattgaggc ggatcagcac cgatcccaga
     4621 ccatcaaatc gtacttcttt ttgcaggcga tccgcttctt ccagtaggat ctgccgcact
     4681 tcctgttccg aggaggcgat cgcatctgcc tcgctcaacg cttttaatag cgataaatcc
     4741 attatgcctc tcccgtgagc aacactgttt tcggcatggc gtagagcact tctgcgcctt
     4801 gcggggtaac cagcacaaca tcttcgatgc gcacgccccc ttgccctggc aaataaatcc
     4861 ccggctccac ggtcagtaac atgcctggct gtagcgtcgt ggtatcccgc ggtgaaaagc
     4921 gtggatcttc gtgtacttca ataccgatag catgaccggt gttgtgacca aaatagtcgc
     4981 caaatcctgc ctcggtaatc acccggcgcg cggcgtcgtc aacctgttag cagcgtacgc
     5041 cagggcgaat cgcggagatt gccgccagct gtgcttgcag gacaatctga tagacgttaa
     5101 acagcgggtg agattcggca attacccctt cgccattcac cagcaaggtg cgcgtcatat
     5161 cagagcagta cccctgatag agcgcaccaa aatcaagagt gacaaactcg cctgctgcaa
     5221 caatcttgtc gctggctttg ccgtgcggca gcgccccacg ccagccgctg gcgacaatgg
     5281 tgtcaaaaga ggctttttct gcgccctgct ggcgcataaa ccactccagt tcagcggcta
     5341 tctcgcgctc gctcatcccc gcctgaataa agcggcgaat atgctccgct ccgcgatcgg
     5401 caatcccaca ggcgaggcgg attatctcca cctcctctgg cgttttgatt tgccgcagca
     5461 catccggcgt ggcgctaacc agtttcgcat tgagttcaga cttccagcgg tgcgcggttt
     5521 cccaactcac ctgctggcct tcaaaaccga gcgtttgcaa ctgctcatcg gcaatgattt
     5581 gattgacgat agtggtaagc gtgttcgtcg cgtcaagcaa atgcagctgg tagccttgcg
     5641 cacgggcttc cacctcaacg aaatagcgcg aatctaccag aatgtgcgca ctttcacggc
     5701 taataaccac atagcctgat ccggtggaga tccccagatg cggctgtttg ttctgccgtg
     5761 aggagagaag cactgcatcc agttgttgcg ccttaagcca gtcgcgcagc gaagcgagta
     5821 atgtcatctg gattttcctt cttacaggct atcgattaac aatttacctt tacggaacat
     5881 catcagacgc aggaacacca ccatcagcgc agttatgact gcccccagcg cgatccccgc
     5941 catatagacg cccaggttag tgaccagcgg ccatgcccag atagcggatt ccgggaacca
     6001 ttgcactgcg cccagccaga cagcggcggt tgagccgaca atcgcgccga ccatatacga
     6061 cggaatggcg gtgatggggc tttccagcgc aaacggaatc gcaccctcac taatccccat
     6121 aaaggcaagg aacatcgcgg tcttaccctg cggataaagt tgagcgttga acaggcgttt
     6181 gccggttaaa cgacggtcga taatggtcgc cagacccaga cctatcggcg gaataacgat
     6241 agcgatagaa cgtgcagtga ccggtaatac gtggtcagtg gtaaagctga aggcaacaaa
     6301 acctgcggct ttgttgatcg ggccaccaag gtcgattgcg gtcgccgcgg cgatccccat
     6361 tgcgtacatc agcgcgccct tctcacctgc ggcagtcagc acggtacgga taccgccgtt
     6421 gatccagccg ccaaaggggg taatgacgta gtacatcgcc agcatgacaa aaatggcgga
     6481 aagaatcggt agcaaaaatg tggttttgaa cgctaacagg aaatccggta actggatttt
     6541 ctggttcatc cacttcacaa ggtaacccgc gacgatagaa ataatcagcg caccaatgaa
     6601 ggtggacggc accggcgaag aggtcgccca ttgcatagtg ctgggatcga agttcagcag
     6661 ttgcgtcggc tgggtagaca tcaagccgcc gataaaacca gccgggaacg ccagtttgcc
     6721 accgatagag ttcgccacaa aagcagcaaa catcggaatg gcaaaaccaa acaacacgcc
     6781 gccgaaggac tgcgacagcc aggcaaattt cagtaaggag aggtcgaaac cggagaattt
     6841 cccgctatta agagcatcca tgatgccgat ctcagcagga attttaagcc agctataagc
     6901 aatcagctgg ctgaaagcga ggatcacacc gcccataatt aaggtcggca ccatgcgtga
     6961 aatccctgac atcacatgct gcgggagttc accccagaag ctgcttttag aggcctgcgg
     7021 attcttaact gccgctgccg caccggatgc gccaggcaca acggttgcac tgcgtttttt
     7081 aatggccata acaattatcc ctgtcgaata ttactgttgt tcagaggcaa tcatctcttc
     7141 gatttctttg atgatgcccg cagcgttttt aattgcgtcc tgcaaagtga tttcataaac
     7201 gtcgcgtgat tcgaaacgct cattatcttc cggggtaact gccacggagt ggatgatgat
     7261 ggttgcttcg gcgatatcct gcgccgtcag gcgattctgg ataccgtccg cgccctgggt
     7321 ttcaattttc acttcataac ctgcttctac cgccgcttct tccagcgcct gagcggccat
     7381 aaaggtgtga gccaggccca ttgggcaggc acataaggca attagtttct tactcatcgt
     7441 taattcctcg ttcagtgata gttgacgagt gaaattatga acgtgcaaaa caaatcgccg
     7501 ttaccagaca aataatgcat ttactggaga attaatcccg ctgtacggaa gtgtgacgca
     7561 ggtcggcctt gtgaggggga cacaaagaaa aattgaatta actgtatgat ttaaaagatt
     7621 atcgggagag ttacctcccg atataacaga aaggatttac agaatgtgcc ctaaggtctg
     7681 gcgtaaatgt gcaccggaac cgagaagacc cggattgtca tggacgatga gatacaccgg
     7741 aatatcatgg acatattctt taaagcgccc tttatcttca aatgcggcac ggaaaccaga
     7801 ggctttgaag aactcaagga agcgcggcac gataccgcca gcaatataaa ccccaccaaa
     7861 ggttcccagc gttaatgcca gattgccgcc aaaacggccc ataatgacgc aaaacagcga
     7921 caacgcacgg cggcaatcgg tgcagctgtc agccagcgcg cgttcggtaa tatcttttgg
     7981 cttgagattt tctggcaggc ggttgtcggc tttaacaatt gcgcgataca aattcaccag
     8041 cccaggacca gaaagcacgc gttccgccga aacatgacca atttccgcac gcaatatttc
     8101 gaggataatg gcctcttctt cactattcgg cgcaaaatca acgtgaccgc cttcgcccgg
     8161 caagcttacc caacgcttat cgacatggac cagatgcgca accccaagcc ctgttccggc
     8221 accgtaaacc gcaataggtt taccttcgac cggttctgcg ccaccaaact gaatcagatg
     8281 ctcttttttc agcatcggga tcgccatcga tacagcggta aaatcgttaa taatttccag
     8341 atggctaaaa ccgagattct ttttcatttc ggcaattgag aacgcccagg tatggttggt
     8401 cattgccacc cagtcaccgg taattgggca agcgatggca atacagccgt ctttcacctc
     8461 gaccttatgt tcttcaagat aaacgcgaat gaccgcttcg aggctggggt aatcaagccc
     8521 tgaataggtc ttagcctgcg agatttcacc actggcaata tcacacagag caagacgtgc
     8581 gttggtgccg cccacatcac cgactaatgc atactttgtc attcttcaac tgctccgcta
     8641 aagtcaaaat aattctttct cacactgtaa atacctgggg gcataacaac aacgccggaa
     8701 aggcaggctc cctgtaaata tcgatctggg tcacacattt actttatcgt ttcagcacca
     8761 attgcagcga tgcctttttg caagctgggc aaactaagta tctgaccccg cataaggaat
     8821 agaacatgct ccatccgcga gccagaacca tgttgttatt atcgctcccc gccgtggcaa
     8881 ttgggattgc gtccagtctt attctgattg tggtgatgaa aatcgcctcg gtattacaga
     8941 atttgctctg gcaacgactg ccgggaactc tggggatagc ccaggattca cctatctgga
     9001 tcatcggtgt attaacgcta acgggtattg cggtggggtt ggttatccgt ttcagccagg
     9061 gtcatgccgg accagacccc gcctgtgaac cgctgatcgg cgcaccagtt ccgccctctg
     9121 cgctaccagg acttatcgta gcattaattc tcggtcttgc tggcggcgtc agcctggggc
     9181 cggaacatcc gatcatgacc gtcaatatcg cccttgccgt cgccattggc gctcgtctgt
     9241 taccgcgcgt caaccgaatg gagtggacta ttttagcctc tgccggaacc atcggcgcgc
     9301 tgtttggtac acctgttgcg gcggcgttga tattttcgca aaccttaaat ggcagtagtg
     9361 aagttccgct atgggatcgt ctttttgcgc cgttaatggc ggcagcagct ggtgcactta
     9421 ctaccggatt atttttccat ccccattttt cactgcccat tgcgcattac ggacagatgg
     9481 aaatgaccga tattctcagc ggtgcaattg tcgcggcgat tgccatcgca gcagggatgg
     9541 tcgccgtatg gtgcttacca cggttgcacg cgatgatgaa tcaaatgaaa aatccggtgc
     9601 tcgtgctggg tattggcgga tttattctcg gaattctggg ggttattggt ggaccagttt
     9661 cgctgtttaa agggctggat gagatgcagc agatggtggc aaatcaggct ttcagcacca
     9721 gcgattactt tttgctggcg gtaattaaac ttgccgccct ggtcgttgct gccgccagtg
     9781 gctttcgcgg tgggcgaatc ttcccggcag tgtttgtcgg cgtggcatta gggttgatgc
     9841 tgcatgagca cgttcccgcc gttccagcgg caataaccgt ttcctgcgct attctcggca
     9901 tcgtgctggt ggtaacacgc gatggctggt taagtctttt tatggcggca gtcgttgtac
     9961 ccaataccac attgctaccg ctgctctgta tcgtcatgct tccggcatgg ctgttattag
    10021 caggtaagcc gatgatgatg gtcaatcgtc cgaagcaaca gccaccccac gataatgttt
    10081 agcaaaaaat gctctccttt atgatttaag agttatggcg atgatacggg gctttacctc
    10141 cccgtaatat tgcgttaaca ggccgctgac ggcttcgatc gtgaaggaga ataacaatgt
    10201 tcaggtcact gtttctggcg gccgccctga tggcatttac cccgcttgca gcaaatgcag
    10261 gtgaaatcac cctactgcca tcgatcaaat tacaaattgg cgatcgcgat cattacggta
    10321 attactggga cggtggtcac tggcgagatc gtgattactg gcatcgcaat tatgaatggc
    10381 gcaaaaaccg ctggtggcgt catgataatg gctatcatcg tggctgggat aagcgtaaag
    10441 catatgagcg tggctatcgt gaaggctggc gcgatcgtga cgatcatcgc ggaaaaggcc
    10501 gcggacatgg gcaccgccat taaacgcgtc ctccaatgga gcacaatgcc tgatgcgacg
    10561 ctagcgcgtc ttatcaggcc tacaaaccgg ctctttaacg cgtagggcgg ataaggcgtt
    10621 tacgccgcat ccgccaatag tgccagatgc gacgctcatt caactacaat cccagcgccg
    10681 tccccaccaa caaccagata ttcagcgcca cgaccagcac tacaatcacc cagcctgtct
    10741 gttttacgcg tttgctgttc accagatcgc ccatcaactt gctgtcactg gtgaaaatca
    10801 gcagtggaac cagcgccagg gcgataccaa aacttaacag cacctgactc ataaccagaa
    10861 tccgtgtcgg atctaatccc atcagaatga caataaatga cggcaacatg gtgactgtac
    10921 gacgcaccca cagcgggata tggaagcgaa tgaatccctg catcaccacc tgccccgcca
    10981 gtgtccccac caccgttgag gacagtccgg cagcaaccag acttaaccca aagaccgttg
    11041 ccgcagcatg gcttaacagc ggttgcagcg tcagataagc ctcatcaaga tcggcaacac
    11101 cagtatgacc agaaaagtgg aacgccgccg cagctgtagc catcatcgcc agattgacaa
    11161 aaccggcaat cgtcatggcg atagccacat cccatttggt ggcggaataa cgttgttgac
    11221 gcgaaccgcc atgtaaatgc tgagtgagcg aagagtgcaa ataaatcaca tgcggcataa
    11281 tcgtcgcccc taacacgcct gctgccagga agaccgcctc cgaagtaggt aaactcggga
    11341 tcaccattcc tttacccagc tgcgccaggt taggctggga gaaaatcaac tcgacaatgt
    11401 aagccgcggc aacaaacaac agtaacccgc caatcacttt ctccagtggt ttttgcccgc
    11461 gacgttgcag cattaaaatc aggaaagtcg cgatccccgt cagtaccgcg ccctgcaaca
    11521 acgagacacc aagaatgagt ttaaaaccga tcgccgcacc aataaattcc gccaggtcgg
    11581 ttgccatcgc aataatttct gcctgaaccc aatagaacca cactacggga cgcggatagt
    11641 gatcacgaat ctgctccgcc agattttttc cggtggcaat ccctagtttg gcagagagga
    11701 tctgaatcag catcgccatc aggttggccc aaacgacaac ccacagtagc tgatagccga
    11761 agctggcacc cgcctgaata ttggtcgcaa agttaccggg atcgatataa ccaatcgccg
    11821 caatgaacgc aggtcccatt aatgcgagcc tcatcttgcg cgccgcccgt ccgctgctac
    11881 tctcaacgcg atagttcgtc atcttgtgcc tctaaaacat agcctttgct atgtttcatg
    11941 ctatgataaa cgagaatgat tatcaaattc atttaaatgg attgtggtga tttctctgat
    12001 agaccaggat tatgactacg aaaagattga tgacttgaat gttatggtta tgtttaatgt
    12061 tagcacattt acataacttt cagcttccat acacaacata gcagaaatgt atgacagatc
    12121 actatttttg aagcctgtca caggacgtca ttatagtgtg tgtcagatct cgttttcctt
    12181 aaccatgtta catagaatgt gcacggaaat ttaacctgcc tcatatttgg agcaaatatg
    12241 gaccgcgtcc ttcattttgt actggcactt gccgttgttg cgattctcgc actgctggta
    12301 agcagcgacc gcaaaaaaat tcgtatccgt tatgttattc aactgcttgt tatcgaagtg
    12361 ttactggcgt ggttcttcct gaactccgac gttggtttag gcttcgtgaa aggcttctcc
    12421 gaaatgttcg aaaaactgct cagatttgcc aacgaaggga ctaacttcgt ctttggtagc
    12481 atgaatgatc aaggcctggc attcttcttc ctgaaagtgc tgtgcccaat cgtctttatc
    12541 tctgcactga tcggtattct ccagcacatt cgcgtgttgc cggtgattat ccgcgcaatt
    12601 ggtttcctgc tctccaaagt caacggtatg ggcaaactgg aatcctttaa cgccgtcagc
    12661 tccctgattc tgggtcagtc tgaaaacttt attgcctata aagatatcct cggcaaaatc
    12721 tcccgtaatc gtatgtacac catggctgcc acggcaatgt ccaccgtgtc gatgtccatc
    12781 gttggtgcat acatgaccat gctggaaccg aaatacgtcg ttgctgcgct ggtactgaac
    12841 atgttcagca cctttatcgt gctgtcgctg atcaatcctt accgtgttga tgccagtgaa
    12901 gaaaacatcc agatgtccaa cctgcacgaa ggtcagagct tcttcgaaat gctgggtgaa
    12961 tacattctgg caggtttcaa agttgccatt atcgttgccg cgatgctgat cggctttatc
    13021 gccctgatcg ccgcgctgaa cgcactgttt gccaccgtta ctggctggtt tggctacagc
    13081 atctccttcc agggcatcct gggctacatc ttctatccga ttgcatgggt gatgggtgtt
    13141 ccttccagtg aagcactgca agtgggcagt atcatggcga ccaaactggt ttccaacgag
    13201 ttcgttgcga tgatggatct gcagaaaatt gcttccacgc tctctccgcg tgctgaaggc
    13261 atcatctctg tgttcctggt ttccttcgct aacttctctt caatcgggat tatcgcaggt
    13321 gcagttaaag gcctgaatga agagcaaggt aacgtggttt ctcgcttcgg tctgaaactg
    13381 gtttacggct ctaccctggt gagtgtgctg tctgcgtcaa tcgcagcact ggtgctgtaa
    13441 aaccatacat aaaaaagccg gggatcattc cccggctttt ttacgcctgt taatcagcta
    13501 atggctgcgg gcgaccaatc aaataccctt gcagatattg caccccgagc ttatgcaata
    13561 gcgcctgctg ctgttgcgtc tcgacaaact ccgcgaccac actcaatgac ttcgctttcg
    13621 ccagatcggt aattgatctc acaatcatcg catccagcgt attcgtgaca atatctttca
    13681 caaagacgcc atcaattttg atgatatcag cctgcaaacg ctttaaccgt tcgtagttgg
    13741 catatccggt gccaaagtca tcaatcgcaa tccggaaacc aaacttatgc agctgctcga
    13801 tgttgtacat gctgctttct gcgttagaaa acgcctgctc ctcggtgatc tcaagaatga
    13861 ccgcctgcgg ggagatgtga taacgcttaa acagacgaat aatccgcccg gcaatattct
    13921 tttgcagcag cgtgagcggc attaaattga ctgaaaagcg cggtcctttt ttgtcgcaag
    13981 ggtgtgttgc cagccacttc aacaaggatt ccagcacttg caaatcaaaa cgcgcgctaa
    14041 ggttaaactg agcaataagg ggcagaaact tatccggggt cataatgccg ccgtcatatt
    14101 tcagtcgcgc gaggatctca tcataacctt caccctcttt gttgcgaatt ggctgggcgt
    14161 agagcagcaa atcaccctga tctaacgccg tgcgaatggt attcagcaat agcacctgtt
    14221 tagtggtctg cccggaaacc atctcctctc tgctatccag cgccagcaca tgatgatgtg
    14281 cgcaggattg ctccgccagc cagcttaact gccccaacaa gggttgcagg gtttcctgat
    14341 ttccatcaaa acgcccccag gcagcaccat agcccatatc cagcccggta ttgttccagt
    14401 gaatttgccg actattcagg atgttaacca tatgctggag tcgcccttcc gtttccggcc
    14461 cgctcagcac taacagcagt tcactacccg gcaattgata taacttttcg ttttcctgca
    14521 tcaacggcag cagcgtacgg taaattgatc ggatacagtg aacgcgcatc attaagccgt
    14581 aatgacgact cataaactca agattatcaa tgcgcaggca gcaaaactct tgcccgcctc
    14641 ttgctccggc gcttgttcca acgcacggaa gttgggcagg agcgttaacg gatcggtcag
    14701 cgcctgcaaa tgccagcggc gattaagcca ttcactgcga tgataaatgc gcaccatata
    14761 gagcaggcaa acgctaaagg aaatcagcac cgcaagaata aatgccagcg aatattcggt
    14821 ttccacccct tgcaaaaagt tctggttgta attcagaagg caaagcgttg aaacagccca
    14881 ggtgagattt aaaaacggat agcgaagctt accgaccccg agggtaaaga tgatgaagaa
    14941 aacgggtacc aggtaaccgg caataaagtc gttttcataa ggtgtgcaca acagaagtag
    15001 aagcacgctt aacgctgcca gccagcttaa ggtaaatgcg cgtttctctt tgcccaacga
    15061 cggcgcgata tccctgcgcc acaatatctg cgcaaagtgg ggacttacaa tcatgcgagt
    15121 gagatagtag aaaagcatgt tgtaaataag cacagcggtg aaaaggctta gcaaatcaac
    15181 gaccgtgaaa atggcatccg catcgccgaa aaaggtggat atctttagcg ggaaatcaaa
    15241 gaagctcccc acaagataca tgctgcattt gatgccgatc ggcgtcacca aaccaagcca
    15301 gaacagacgt tgccagacat atcggctggt caatccataa cgccaacgcg tccccagctg
    15361 ccagcgtaat attgcgcagg cacacaacac gacaaacgtc tggctaccga gcatgaccgc
    15421 agtctgtaaa aaagagagat gaaagttcca cagattggtg caaaacatcc cgcataaaac
    15481 gggaacgaca ccgcgccagc caaagataaa caagattgac agcattacgc acagtggcat
    15541 ccacgccaga aagatataac tggaatggat aatggcaagt ggcgaaataa aacgggatag
    15601 ctgaataagt accacggtga gcgtaaacgc tagtgtgaat atcttgatat tttttatcag
    15661 gttatgctcc acaaacatga gtacactact ctcactgatc ggggttttca ggcacacaaa
    15721 taataagttt attcgcatcg cgaagcaaac agggggccag cctgttgccg atttttcatc
    15781 caatgatgct ttcaccaggc ggcactaacc cggacgtaga ctgtattaca aaagcggcaa
    15841 aaagcagaga caaaaaaccc ccgctttgca gcgagggttg gaaatttggt ggagctaagc
    15901 gggatcgaac cgctgacctc ttgcatgcca tgcaagcgct ctcccagctg agctatagcc
    15961 ccacgatgcg tttacgtacc aaatttgctg ggtgcaaaat ttggtggagc taagcgggat
    16021 cgaaccgctg acctcttgca tgccatgcaa gcgctctccc agctgagcta tagccccgtc
    16081 acgtaaagct tgtcgagttg acgggcggca tcatatgaat tccgcccgaa tgtgtcaacg
    16141 gcaaattgca acgtgtagtt tcaatcgctg aaaaatcagg caaatgaaca attttggaaa
    16201 accgctcgca ttcagtagtt attcatgtca cggtttcctg taaagtggtg ttataaaatg
    16261 aactactaat agacccacat acattcaggg aattgttatg ttcaaggaga ggatgacacc
    16321 agatgaactt gccagactga ccggctatag ccgccagacc attaataaat gggtacgcaa
    16381 ggagggctgg acaacgtcac caaaacctgg cgtccagggt ggcaaagccc gactggttca
    16441 cgtcaatgaa caggttcgtg aatatattcg caatgctgaa cgtccagaag gtcagggaga
    16501 agcgcctgcc ctttctggtg atgcgccgct tgaagtattg ctggtgacac tggcgaaaga
    16561 gatgacgcca gttgaacaaa aacagtttac atccttgctt ctgcgggaag ggattatcgg
    16621 attgttacaa cgcttaggga ttcgcgatag caaataatat gaaaagatta cgcaataaaa
    16681 tgaccaccga agaactggct gaatgcctcg gtgtggctaa acaaactgtt aaccgttgga
    16741 tcagagaaaa aggctggaaa acggaaaaat ttcccggcgt gaaaggcggt cgtgccagac
    16801 ttattctggt cgatacgcaa gtttgcgagt ttattcagaa cacgccagcc ttccataaca
    16861 caccaatgtt gatggaagct gaagagagaa ttgctgaata cgctcctgga gcacgcgccc
    16921 cggcgtatcg acaaatcatc aacgcgatag ataatatgac agacattgag cagggaaaag
    16981 tcgcgcaatt tttgtcacgt gaaggaattc gcaatttcct cgcccgtctc gatattgacg
    17041 aatcagcata aataaaaaac ggcaggatat tatctcctgc cgtttatctt tttacacgct
    17101 aattactgct gattttcgcg ttcagcaata aaatccagcg ctttgttgat acgctcgata
    17161 ctgcgggtct taccaattgc gtgaacggta acatccagtg ctggagactg ccccgccccg
    17221 gttacggcga cacgcagcgg cataccaact ttacccatac ccacttccag ctcatccgcc
    17281 gtcgcctgaa tagcgtgatg aacgttttca gcggtccagt cagtaatcgc ggccagtttg
    17341 tcacgaacca cttccagcgg ctgacgcgct accggacgca gatgtttttt cgccgcatcg
    17401 gcatcgaact cagcaaaatc ttcgtagaaa taacggcagc tctgtgccat ctctttcagc
    17461 gtcttgcagc gttcgcccag cagtttcacc agatcagcca gctgcgggcc gttacgggta
    17521 tcgatatttt cctgctcaat gtgccactgt aagtgagtag caacatactc cggcggcagc
    17581 gcgttaatgt agtgatggtt cagccacagc agcttgtcgg tgttgaacgc actggcagat
    17641 ttgctgacgg cattcaaagt gaagtatttg atcatctctt cacgagtgaa gatttcctga
    17701 tcgccgtggg accagcccag acgcaccaga tagttcagca gtgcttctgg caaataaccg
    17761 tcatcacgat actgcattac gctgactgcc ccgtgacgtt tggacagttt tttaccgtca
    17821 tcgccattga tcatagaaac gtgcgcgtaa accggcaccg gcgctttcag ggccttaaga
    17881 atgttgatct ggcgcggcgt gttgttgata tggtcttcgc cacggataac gtgggtgatt
    17941 tccatatccc agtcatcgac aaccacacag aagttatagg ttggggaacc atcggtacgg
    18001 cggataataa gatcgtccag ttcctggttg ctgaactcga tcggaccacg gatctgatcg
    18061 tcaaaaacaa cagaaccttc ctgcgggtta gcaaaacgta caacacacgg ttcatcatca
    18121 gcatgatgct catggctgtg gcggcagcga ccgtcataac gcggcttctc acctttcgcc
    18181 atttgctctt cgcgcagcgc ttccaggcgc tctttagagc aatagcattt ataagcagtg
    18241 ccctcttcca gcatctgatc gatcaccgcg ttgtagcgat caaaacgttt ggtctggtag
    18301 tacggacctt catcccactc caggctcagc cagttcatgc catccataat ggcttcgata
    18361 gcttccggcg tggaacgctc aagatcggtg tcttcaatac gcagcacgaa ctcaccgccg
    18421 tggttacgtg caaaaagcca ggagtaaaga gcagtacgcg cgccgccaac gtgcagatag
    18481 cctgttgggc ttggcgcgaa gcgagttttg attttcatga aatggcctta cgtttagaaa
    18541 gatgccgaca accggcaaat cctggaaaaa ttgagtgggc gatattctat cactcccgcc
    18601 tgattcctca atgtagttcg ggtttctacc ttacgcatta ttgttttcgt tgacaaattg
    18661 cgcaacacgg tctgttttgc gatcatcttg cttaaattta cgacgaacga acaatttctt
    18721 tagaaaatgc gttgactcat tttgaactct ccctataatg cggctccaca cagcgggggt
    18781 gattagctca gctgggagag cacctccctt acaaggaggg ggtcggcggt tcgatcccgt
    18841 catcacccac caactacttt atgtagtctc cgccgtgtag caagaaattg agaagtgggt
    18901 gattagctca gctgggagag cacctccctt acaaggaggg ggtcggcggt tcgatcccgt
    18961 catcacccac caatttctcg ccagctaaat ttcttgtaaa aatgtgaagt accgaagtgg
    19021 gtgattagct cagctgggag agcacctccc ttacaaggag ggggtcggcg gttcgatccc
    19081 gtcatcaccc accacttcgg gtcgttagct cagttggtag agcagttgac ttttaatcaa
    19141 ttggtcgcag gttcgaatcc tgcacgaccc accaatgtaa aaaagcgccc taaaggcgct
    19201 tttttgctat ctgcgatttg taagattgat ttttcttctc aagttccttg ttcttctgcc
    19261 gatattacct cgtacagtga cgaggactga acaatggcca ctatctcttc aacttcaatt
    19321 ccttccatac aaacacagag tagtaacagg gcatcacaag gtagcgatgt tgcttcaaaa
    19381 atcgcgcgga taagtcagca aatcatcaaa ctcactcaac aaattaaaga gattgtagac
    19441 actagcggca gtgctgaaga caaacaaaaa caggccgaat tgattcaaca gcaaatcacc
    19501 ctactggaaa cacaactggc gcagttgcag agacagcagg cagaaaaggc gcaggagaaa
    19561 gagcagcgtt tgtctctgaa tgtatccctt ctaaatcctg ttgaaaatac gactcatatc
    19621 gatatctata tctgatacaa cggagcccct tcccgaccag ccattagcgc attgccctgg
    19681 tctcgtacca cgtcccataa cgcttcagcg gcggtggaaa gtgaacggtt cttcctgcgc
    19741 accagcatca actggcgttc aacaaccggc gtaatgcgct ttaccaccag tgggctacct
    19801 tcaggcaatg gtagcgccag tgcgggaaga atactgatgc cgatgcctgc ggctaccatc
    19861 gggaacagcg tcgccggatg tccaatctcc tgcacaatat tcgcctgaat accattgcgc
    19921 gccagcgctg cgtcaatcag cggtcggctg cctgacgcgt aatcctgcaa caccaatttc
    19981 gctccctgta atgcctgcca gggtacataa tcttccacgg ccaaagcgct atcgcgatgg
    20041 caaagcagaa agaaaggctc ggaaagaatc gcttcacatt gcagatcgcc cactgggcct
    20101 ggatcgatga cgatgccaaa atcgacatct ccctggcgaa tactttccat cacccactgc
    20161 tgcggacgat cgtgcaggac aaactgaata tctggatagc ggcgatggct ttcagcaatg
    20221 cattgcggaa taagatgcgc ggaaatggtc tggctggcag cgacccgaac ttttccgctc
    20281 agttgttgcc ccatacgccc ggtatcgcgc aacgtgctgt taagttcatc cagcagtcgc
    20341 tcaagacgca aagccaactg ctgccctgca tctgtaagca ccacttcacg cgtggttctg
    20401 tccagcaggc gaacaccagt atgattttcc agctccttca cactgtgact tactgccgac
    20461 tggctcaagc cgatacgctc tcctgcacga ctaaaacttt tctcctgcgc tactgtgacg
    20521 aaaaccttta attgttttaa agaataattc atctgttttc ttcatgaatg gatgcaataa
    20581 atcaatttta tttctcaaac tggaagaagc acaatagaac catcgatcat ctggagtctt
    20641 tatgaaactt tttcgtatcc tcgatccttt caccttaacc ctgatcacgg tggtgttgct
    20701 ggcctctttc tttccggcca gaggcgattt cgtccccttc tttgaaaatc tgaccaccgc
    20761 agctattgcc ctgctgttct ttatgcacgg cgcgaagttg tcgcgtgagg cgattattgc
    20821 tggcggtggt cactggcgac tgcatttgtg ggtaatgtgc agcaccttcg tgctgtttcc
    20881 gattctgggt gtactgtttg cctggtggaa accggtaaat gtcgacccga tgctctactc
    20941 cggttttctc tacttgtgca ttctcccggc taccgtgcag tctgcaatcg ccttcacgtc
    21001 aatggcgggc ggtaacgtcg cggcggcggt ttgttctgcg tcagcatcca gcctgctggg
    21061 gattttcctt tcaccattgc tagttggtct ggtgatgaat gttcacggtg cagggggcag
    21121 ccttgagcag gtcggtaaaa ttatgctgca actgctgctg ccgtttgtgt tggggcatct
    21181 ttcccggccg tggattggtg actgggtgtc gcgcaataaa aaatggattg cgaaaactga
    21241 ccagacgtcc attctgttgg tggtttatac agcgttcagc gaagccgtcg ttaatggtat
    21301 ctggcataaa gttggctggg gatcattgct gtttatcgtg gtggtcagct gcgttcttct
    21361 ggctatcgtg attgtagtta acgtctttat ggcacgccga ctgggcttca ataaggcaga
    21421 tgaaattact atcgtctttt gtggttcgaa aaagagtctg gcaaatggca tcccgatggc
    21481 aaacattctg ttccccacat cggtgatcgg tatgatggtg ctgcccctga tgattttcca
    21541 tcagatccaa ttgatggtct gtgcggtgct ggcgcgtcga tacaaacgcc agaccgaaca
    21601 gttacaggcg cagcaggaaa gcagcgccga taaagcttaa agcggacgct tcaggggctg
    21661 gaccagttgc gtcagcccct cggttttaat cagcagcgtg atttgcatta gctcaccgag
    21721 cttccctgcc gggaactcat ctttgcgggc aaaccacagc agatactcct ccggcaggtc
    21781 gattaagcga cgccctttgt atttgccaaa cggcattatc gtattggcta tttcaatcag
    21841 ctgctctttt tccatctcag ctacccagca aacgcagcat ttccgcttcg tcgatgactt
    21901 caatgcccag ttcctgcgcc ttcgccagtt tagatcctgc agcttcaccc gctatcacca
    21961 gatcggtttt cttcgacacg ctgcccgcga ctttcgcgcc cagttcgacc agtcgagctt
    22021 tagcgtcatc acgcgacatc tggcttaagc tgcccgtaag caccacggtt ttaccagcaa
    22081 acgggctgtc aatctcttcc gcgttgataa cgatcggcgc aggccagtga acaccttccg
    22141 ccaacagctc gctgatgaca ttgcggttgc tttcttcggc aaagaagttg tgaacgtggg
    22201 atgcaacgac aatgccaaca tcaggcacct tttgcagctc ttcaatcgaa gcggcttcca
    22261 gcacttccag cgtgccgaaa tatgccgcca gacctgctgc ggtggcctcg ccgacttcac
    22321 ggatgccaag tgcatagagg aagcgagcaa aggtggtttc tttcgctttt tccagcgcgt
    22381 taaccacgtt ttgtgccgat tttggcccca tacgctccag tccggtcagt ttgcctgcgg
    22441 tgagtttgaa cagatctgcc ggagtgtgga catattcttt ttcaaccagc tgatcgatga
    22501 ttttgtcgcc cattccgtca acatccatcg cacggcggga aacaaagtgt ttcagcgact
    22561 ctttacgctg cgcaccgcaa atcaggccac cggtacagcg ggcaaccgct tcaccttcca
    22621 cacgctcaac gtcagaacca cataccggac aatgcgtcgg gaatacaacc tcacgggtat
    22681 cttccgggcg ttcagaaagc acgacgttaa ccacctgcgg gatcacgtcg ccagcgcggc
    22741 gaatcaccac tttatcgcca atgcgtaaac caagacgttc gatttcatcc gcattgtgta
    22801 aggttgcgtt actcaccagc acgccagcaa catggacagg ttccagacgc gcaacaggcg
    22861 taatcgcgcc agtacgccca acctgaaact cgacgtcacg cacaaaggtc atctgctcct
    22921 gcgccgggaa tttaaacgct accgcccagc gcggggcacg cgcgacaaag ccaagctgct
    22981 cctgctgttc cagtgagttg accttaatca ccacgccgtc gatatcaaag cccagcgtcg
    23041 ggcggtcttc ttccactttg tgatagaacg ccagcacttc ttccgccgat tcacaaagcg
    23101 ttacccgatc gctgaccggc aacccccact ttttaaattg cagtaaacgg ccaagatgag
    23161 tatccggcag ctcgccacct tccagaacac caacgccata gcagaaaaaa gtgagcggtc
    23221 gcttcgctgt aatacgcgga tcaagctgac gcagtgaacc agctgccgca ttacgtgggt
    23281 tagcaaaaac tttcccgccc gtgcgtcgcg catcttcgtt aatcttttcg aaccccgcct
    23341 gcggcaggaa cacttcacca cgcacttcca gacgcgccgg gatattctct ccgtgcagct
    23401 tcagcggaat ggcgcgaata gtacgcacat tagacgtgat atcttccccg gtggtgccat
    23461 cgccacgggt cgcggcactg actaaaacgc cattttcata cagaatactg acggcaaggc
    23521 catccagctt cagctcacag caccaggtga ctttctcgtt gtttttcaga cggtcctgca
    23581 cacgtttgtt gaaagcaaga aagctttctt catcaaaaac gttatccagt gacagcattg
    23641 gtacttcatg gcgtatctgg ctgaaagccg ccagcggcgc agcacctaca cgttgggtag
    23701 gcgaatcagg cgtaatcagt tctggatgtt tggtttccag ctcgcgcagt tcgcgcatca
    23761 gcctgtcgta ttcagcgtcg ggaatttccg gcgcatccat cacatgataa agatattcat
    23821 gatggcgaag cgtcgttcgc agttctgtca gttgttgttc gattgattcc atatcgcacc
    23881 atcaatgcta aaaacccccg acaagcgggg gttcgaagag gagttaattt gccttaagtg
    23941 tatcaggcgt tggcgtcttt gacttcgcgg atgatgtcct ggtactcgcg caatttctgc
    24001 ggagtcatca tacggcgctg atcgtcaagc acgacaccgc ccacttcatc ggcaatatgc
    24061 tgcgcagatt gcagcatcag cttgaagttc tgcagctcgt caccgtaaga cggtacctgc
    24121 ataaagatag tgacacccgg agtagtgaaa tccttcattt caggatcaaa ggttcccggt
    24181 ttcaccatat tcgccaggct gaataacgcc gggccgctgc catccgggct aagatgacga
    24241 tggtaaatat tcatatcgcc aaaaatgaag cccgcttgtt gaatgctgtt aagaagcagt
    24301 tcaccgttta gctcgctacc gtgatgcgcc gcgacgttca tgataatcac cgcttctttg
    24361 cgcttcggtt tatccataac tggagccggt tccgctacag gctcaggctg tggtgccgct
    24421 acgggttctg caggctggaa agcctgttgt gccggttgcg gtgctgaatg cacaggctgc
    24481 ggcgctggcg cgacctgtgg cgaaactggc tgctgcaacg gctgttcagg ctgcggctga
    24541 taggcaggct gctgcatcgg ctgcgctgga cgcggagcat gttgcggcgg tacctgcgct
    24601 tcaggcggct gctggatcgg ttgacgcggc tgcgcagacg cataaggcgg ttggtactgg
    24661 tgttgcggcg acggacgagc agcctcatgc tcctgagcgt tagccggggc atgattcacg
    24721 cggtgaacac gaacctcacc aacgccctca tcatcttcga catcctcgtc ataagaatcg
    24781 tcgtcacgtt ttgacttcat tcgttttaat ggccgatcgc ggaacataga agatcgttct
    24841 ttacggctgg tccagaaacc atgtaccagt aaagcgatta tggcgatcgc gccaacaatg
    24901 attaatatca gacgcaaatc ctgcatcatt atattctctg ttgttctaac accttgccac
    24961 cacggcaaac atttactcac taagagtatt tgccgattac ctcaagtgca agtgcactat
    25021 taactttcac agcacaaaga tagatgaaat cgtgcttttt gctgtttttt cgaacatatc
    25081 ctaactgtcc attgcgcaat tacccggtaa aatacgcaga attttcctgg gattggtcaa
    25141 aaggagctca tcctgattat ggtttcatca ttcacatctg ccccacgcag cggtttttac
    25201 tattttgcgc aaggctggaa gctcgtctcg caacctggga ttcggcgttt cgttatttta
    25261 ccgctgctgg tcaatatttt gttgatgggg ggcgcattct ggtggctctt tacacagctc
    25321 gatgtctgga tcccgactct catgagttac gttccggact ggctacaatg gctgagttat
    25381 ctgttgtggc ctctggctgt catctctgtg ctgttagtgt ttggctattt cttctccacg
    25441 attgctaact ggattgccgc tccgtttaac ggtttattgg ctgaacaact ggaagcacga
    25501 ttgactggcg ctacaccgcc agataccggg attttcggta tcatgaaaga tgtgccgcga
    25561 atcatgaaac gcgaatggca aaaatttgcc tggtatctgc cgcgcgcaat tgtattacta
    25621 attctttact tcatccctgg tattgggcaa accgtcgcgc cggtactgtg gttcctgttt
    25681 agcgcctgga tgttagccat ccagtattgc gattacccct tcgataacca caaagtgccg
    25741 tttaaagaga tgcgcaccgc cctgcgcaca cgcaaaatca ccaatatgca gtttggtgct
    25801 ttaaccagcc tgtttacgat gatcccgctg cttaatctgt tcatcatgcc cgttgccgtt
    25861 tgtggcgcga cggcgatgtg ggtcgattgc tatcgcgata aacacgcgat gtggcggtaa
    25921 caatctaccg gttattttgt aaaccgtttg tgtgaaacag gggtggctta tgccgcccct
    25981 tattccatct tgcatgtcat tatttccctt ctgtatatag atatgctaaa tccttacttc
    26041 cgcatattct ctgagcgggt atgctacctg ttgtatccca atttcataca gttaaggaca
    26101 ggccatgagt aagatttttg aagataactc gctgactatc ggtcacacgc cgctggttcg
    26161 cctgaatcgc atcggtaacg gacgcattct ggcgaaggtg gaatctcgta accccagctt
    26221 cagcgttaag tgccgtatcg gtgccaacat gatttgggat gccgaaaagc gcggcgtgct
    26281 gaaaccaggc gttgaactgg ttgaaccgac cagcggtaat accgggattg cactggccta
    26341 tgtagctgcc gctcgcggtt acaaactcac cctgaccatg ccagaaacca tgagtattga
    26401 acgccgcaag ctgctgaaag cgttaggtgc aaacctggtg ctgacggaag gtgctaaagg
    26461 catgaaaggc gcaatccaaa aagcagaaga aattgtcgcc agcaatccag agaaatacct
    26521 gctgctgcaa caattcagca atccggcaaa ccctgaaatt cacgaaaaaa ccaccggtcc
    26581 ggagatctgg gaagataccg acggtcaggt tgatgtattt attgctggcg ttgggactgg
    26641 cggtacgctg actggcgtca gccgctacat taaaggcacc aaaggcaaga ccgatcttat
    26701 ctctgtcgcc gttgagccaa ccgattctcc agttatcgcc caggcgctgg caggtgaaga
    26761 gattaaacct ggcccgcata aaattcaggg tattggcgct ggttttatcc cggctaacct
    26821 cgatctcaag ctggtcgata aagtcattgg catcaccaat gaagaagcca tttctaccgc
    26881 ccgtcgtcta atggaagaag aaggtattct tgcaggtatc tcttctggag cagctgttgc
    26941 cgcggcgttg aaactacaag aagatgaaag ctttaccaat aagaatattg tggttattct
    27001 accatcatcg ggtgagcgtt atttaagcac cgcattgttt gccgatctct tcactgagaa
    27061 agaattgcaa cagtaatgcc agcttgttaa aaatgcgtaa aaaagcacct ttttaggtgc
    27121 ttttttgtgg cctgcttcaa actttcgccc ctcctggcat tgattcagcc tgtcggaact
    27181 ggtatttaac cagactaatt attttgatgc gcgaaattaa tcgttacagg aaaagccaaa
    27241 gctgaatcga ttttatgatt tggttcaatt cttcctttaa cggcataatg tttaatgacg
    27301 tacgaaacgt cagcggtcaa cacccgccag caatggactg tattgcgctc ttcgtgcgtc
    27361 gcgtctgtta aaaactggcg ctaacaatac aggctaaagt cgaaccgcca ggctagactt
    27421 tagttccaca acactaaacc tataagttgg ggaaatacaa tgttccagca agaagttacc
    27481 attaccgctc cgaacggtct gcacacccgc cctgctgccc agtttgtaaa agaagctaag
    27541 ggcttcactt ctgaaattac tgtgacttcc aacggcaaaa gcgccagcgc gaaaagcctg
    27601 tttaaactgc agactctggg cctgactcaa ggtaccgttg tgactatctc cgcagaaggc
    27661 gaagacgagc agaaagcggt tgaacatctg gttaaactga tggcggaact cgagtaattt
    27721 cccgggttct tttaaaaatc agtcacaagt aaggtagggt tatgatttca ggcattttag
    27781 catccccggg tatcgctttc ggtaaagctc tgcttctgaa agaagacgaa attgtcattg
    27841 accggaaaaa aatttctgcc gaccaggttg atcaggaagt tgaacgtttt ctgagcggtc
    27901 gtgccaaggc atcagcccag ctggaaacga tcaaaacgaa agctggtgaa acgttcggtg
    27961 aagaaaaaga agccatcttt gaagggcata ttatgctgct cgaagatgag gagctggagc
    28021 aggaaatcat agccctgatt aaagataaac acatgacagc tgacgcagct gctcatgaag
    28081 ttatcgaagg tcaggcttct gccctggaag agctggatga tgaatacctg aaagaacgtg
    28141 cggctgacgt acgtgatatc ggtaagcgcc tgctgcgcaa catcctgggc ctgaagatta
    28201 tcgacctgag cgccattcag gatgaagtca ttctggttgc cgctgacctg acgccgtccg
    28261 aaaccgcaca gctgaacctg aagaaggtgc tgggtttcat caccgacgcg ggtggccgta
    28321 cttcccacac ctctatcatg gcgcgttctc tggaactgcc tgctatcgtg ggtaccggta
    28381 gcgtcacctc tcaggtgaaa aatgacgact atctgattct ggatgccgta aataatcagg
    28441 tttacgtcaa tccaaccaac gaagttattg ataaaatgcg cgctgttcag gagcaagtgg
    28501 cttctgaaaa agcagagctt gctaaactga aagatctgcc agctattacg ctggacggtc
    28561 accaggtaga agtatgcgct aacattggta cggttcgtga cgttgaaggt gcagagcgta
    28621 acggcgctga aggcgttggt ctgtatcgta ctgagttcct gttcatggac cgcgacgcgc
    28681 tgcccactga agaagaacag tttgctgctt acaaagcagt ggctgaagcg tgtggctcgc
    28741 aggcggttat cgttcgtacc atggacatcg gcggcgacaa agagctgcca tacatgaact
    28801 tcccgaaaga agagaacccg ttcctcggct ggcgcgctat ccgtatcgcg atggatcgta
    28861 aagagatcct gcgcgatcag ctccgcgcta tcctgcgtgc ctcggctttc ggtaaattgc
    28921 gcattatgtt cccgatgatc atctctgttg aagaagtgcg tgcactgcgc aaagagatcg
    28981 aaatctacaa acaggaactg cgcgacgaag gtaaagcgtt tgacgagtca attgaaatcg
    29041 gcgtaatggt ggaaacaccg gctgccgcaa caattgcacg tcatttagcc aaagaagttg
    29101 atttctttag tatcggcacc aatgatttaa cgcagtacac tctggcagtt gaccgtggta
    29161 atgatatgat ttcacacctt taccagccaa tgtcaccgtc cgtgctgaac ttgatcaagc
    29221 aagttattga tgcttctcat gctgaaggca aatggactgg catgtgtggt gagcttgctg
    29281 gcgatgaacg tgctacactt ctgttgctgg ggatgggtct ggacgaattc tctatgagcg
    29341 ccatttctat cccgcgcatt aagaagatta tccgtaacac gaacttcgaa gatgcgaagg
    29401 tgttagcaga gcaggctctt gctcaaccga caacggacga gttaatgacg ctggttaaca
    29461 agttcattga agaaaaaaca atctgctaat ccacgagatg cggcccaatt tactgcttag
    29521 gagaagatca tgggtttgtt cgataaactg aaatctctgg tttccgacga caagaaggat
    29581 accggaacta ttgagatcat tgctccgctc tctggcgaga tcgtcaatat cgaagacgtg
    29641 ccggatgtcg tttttgcgga aaaaatcgtt ggtgatggta ttgctatcaa accaacgggt
    29701 aacaaaatgg tcgcgcctgt agacggtacc attggtaaaa tctttgaaac caatcacgcg
    29761 ttctctatcg aatctgatag cggcgttgaa ctgttcgtcc acttcggtat cgacaccgtt
    29821 gaactgaaag gcgaaggctt caagcgtatt gctgaagaag gtcagcgcgt gaaagttggc
    29881 gatactgtca ttgaatttga tctgccgctg ctggaagaga aagccaagtc taccctgact
    29941 ccggttgtta tctccaacat ggacgaaatc aaagaactga tcaaactgtc cggtagcgta
    30001 accgtgggtg aaaccccggt tatccgcatc aagaagtaat tcttgccgca gtgaaaaatg
    30061 gcgcccatcg
//
LOCUS       DAKISA010000046        29975 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.046, whole genome shotgun sequence.
ACCESSION   DAKISA010000046 DAKISA010000000
VERSION     DAKISA010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29975
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.046"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            282..665
                     /gene="secE"
                     /locus_tag="OVW45_002139"
     CDS             282..665
                     /gene="secE"
                     /locus_tag="OVW45_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCV5526582.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            667..1212
                     /gene="nusG"
                     /locus_tag="OVW45_002140"
     CDS             667..1212
                     /gene="nusG"
                     /locus_tag="OVW45_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCV5526583.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1371..1799
                     /gene="rplK"
                     /locus_tag="OVW45_002141"
     CDS             1371..1799
                     /gene="rplK"
                     /locus_tag="OVW45_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCV5526584.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1803..2507
                     /gene="rplA"
                     /locus_tag="OVW45_002142"
     CDS             1803..2507
                     /gene="rplA"
                     /locus_tag="OVW45_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCV5526585.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2920..3417
                     /gene="rplJ"
                     /locus_tag="OVW45_002143"
     CDS             2920..3417
                     /gene="rplJ"
                     /locus_tag="OVW45_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCV5526586.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3484..3849
                     /gene="rplL"
                     /locus_tag="OVW45_002144"
     CDS             3484..3849
                     /gene="rplL"
                     /locus_tag="OVW45_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCV5526587.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKSLEEAGAEVEVK"
     gene            4169..8197
                     /gene="rpoB"
                     /locus_tag="OVW45_002145"
     CDS             4169..8197
                     /gene="rpoB"
                     /locus_tag="OVW45_002145"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCV5526588.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8274..12497
                     /gene="rpoC"
                     /locus_tag="OVW45_002146"
     CDS             8274..12497
                     /gene="rpoC"
                     /locus_tag="OVW45_002146"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCV5526589.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12739..13872)
                     /gene="thiH"
                     /locus_tag="OVW45_002147"
     CDS             complement(12739..13872)
                     /gene="thiH"
                     /locus_tag="OVW45_002147"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCV5526590.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13869..14639)
                     /gene="thiG"
                     /locus_tag="OVW45_002148"
     CDS             complement(13869..14639)
                     /gene="thiG"
                     /locus_tag="OVW45_002148"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCV5526591.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(14641..14841)
                     /gene="thiS"
                     /locus_tag="OVW45_002149"
     CDS             complement(14641..14841)
                     /gene="thiS"
                     /locus_tag="OVW45_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCV5526592.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14825..15580)
                     /gene="thiF"
                     /locus_tag="OVW45_002150"
     CDS             complement(14825..15580)
                     /gene="thiF"
                     /locus_tag="OVW45_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCV5526593.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVVLNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15573..16208)
                     /gene="thiE"
                     /locus_tag="OVW45_002151"
     CDS             complement(15573..16208)
                     /gene="thiE"
                     /locus_tag="OVW45_002151"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCV5526594.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     NEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16208..18103)
                     /gene="thiC"
                     /locus_tag="OVW45_002152"
     CDS             complement(16208..18103)
                     /gene="thiC"
                     /locus_tag="OVW45_002152"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCV5526595.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18184..18284)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18337..18813)
                     /gene="rsd"
                     /locus_tag="OVW45_002153"
     CDS             complement(18337..18813)
                     /gene="rsd"
                     /locus_tag="OVW45_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCV5526596.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18908..19681
                     /gene="nudC"
                     /locus_tag="OVW45_002154"
     CDS             18908..19681
                     /gene="nudC"
                     /locus_tag="OVW45_002154"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCV5526597.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19721..20785
                     /gene="hemE"
                     /locus_tag="OVW45_002155"
     CDS             19721..20785
                     /gene="hemE"
                     /locus_tag="OVW45_002155"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCV5526598.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20795..21466
                     /gene="nfi"
                     /locus_tag="OVW45_002156"
     CDS             20795..21466
                     /gene="nfi"
                     /locus_tag="OVW45_002156"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCV5526599.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21509..22099
                     /locus_tag="OVW45_002157"
     CDS             21509..22099
                     /locus_tag="OVW45_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCV5526600.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22286..22558
                     /gene="hupA"
                     /locus_tag="OVW45_002158"
     CDS             22286..22558
                     /gene="hupA"
                     /locus_tag="OVW45_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCV5526601.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22571..23266
                     /locus_tag="OVW45_002159"
     CDS             22571..23266
                     /locus_tag="OVW45_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCV5526602.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23268..23693)
                     /gene="zraP"
                     /locus_tag="OVW45_002160"
     CDS             complement(23268..23693)
                     /gene="zraP"
                     /locus_tag="OVW45_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCV5526603.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            23931..25307
                     /gene="zraS"
                     /locus_tag="OVW45_002161"
     CDS             23931..25307
                     /gene="zraS"
                     /locus_tag="OVW45_002161"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCV5526604.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLDAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVT
                     ITRKGPQG"
     gene            25304..26629
                     /gene="zraR"
                     /locus_tag="OVW45_002162"
     CDS             25304..26629
                     /gene="zraR"
                     /locus_tag="OVW45_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCV5526605.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADRRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAVHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26626..27915)
                     /gene="purD"
                     /locus_tag="OVW45_002163"
     CDS             complement(26626..27915)
                     /gene="purD"
                     /locus_tag="OVW45_002163"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCV5526606.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(27927..29516)
                     /gene="purH"
                     /locus_tag="OVW45_002164"
     CDS             complement(27927..29516)
                     /gene="purH"
                     /locus_tag="OVW45_002164"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCV5526607.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7016 a         7816 c         8263 g         6880 t
ORIGIN      
        1 tgaaggcggc cgtaccgttg gcgcgggcgt tgtagcaaaa gttctgagct aattgccgat
       61 aacatttgac gcaatgcgca ctaaaagggc atcatttgat gccctttttg cacgctttcg
      121 taccagaacc tggctcatca gtgattttct ttgtcataat cattgctgag acaggctctg
      181 ttgagggcgt ataatccgaa aagctaatac gcgtttcgat ttggtttgcc tcgcgatcgc
      241 ggggtgaaaa tgtttgtaga aaacttctga caggttggtt tatgagtgcg aataccgaag
      301 ctcaaggaag cgggcgcggc ctggaagcga tgaagtgggt cgttgtggtg gcattgctcc
      361 tggtggcgat tgtcggcaac tacctttatc gcgacattat gctgccgctg cgtgcgctgg
      421 ccgtagtaat tctgattgct gcagcgggtg gtgtcgcgct gttaacgaca aaaggtaaag
      481 ctaccgttgc ttttgcccgt gaagcgcgta ccgaagtccg taaggtcatt tggccgactc
      541 gccaggaaac attgcacacc acgctgattg tggctgcggt taccgcagta atgtcactga
      601 tcctgtgggg actggatggt attctggttc gcctggtatc ctttatcact ggcctgaggt
      661 tctgagatgt ctgaagctcc taaaaagcgc tggtacgtcg ttcaggcgtt ttccggtttt
      721 gaaggccgcg tagcaacgtc gctacgtgag catatcaaat tacacaacat ggaagatttg
      781 tttggtgaag tcatggtacc aaccgaagaa gtggttgaaa tccgtggcgg tcagcgtcgc
      841 aaaagcgaac gtaaattctt ccctggctac gtcctcgttc agatggtgat gaacgacgcg
      901 agctggcacc tggtgcgcag cgtaccgcgt gtgatgggct tcatcggcgg tacttccgat
      961 cgtcctgcgc caatcagcga taaagaagtc gatgcgatta tgaaccgcct gcagcaggtt
     1021 ggtgataagc cgcgtccgaa aacgctgttt gaaccgggtg aaatggtccg tgttaatgat
     1081 ggtccgttcg ctgacttcaa cggtgttgtt gaagaagtgg attacgagaa atctcgtctg
     1141 aaagtgtctg tttctatctt cggtcgtgcg accccggtag agctggactt cagccaggtt
     1201 gaaaaagcct aacccagcga tcaaaaaagc ggcgatttaa tcgttgcaca aggcgtgaga
     1261 ttggaataca atttcgcgcc ttttgttttt atgggccttg cccgtaaaac gattttttat
     1321 atcacgggga gcctctcaga ggcgttatta cccaacttga ggaatttata atggctaaga
     1381 aagtacaagc ctatgtcaag ctgcaggttg cagctggtat ggctaacccg agtccgccag
     1441 taggtccggc tctgggtcag cagggcgtaa acatcatgga attctgcaaa gcgttcaacg
     1501 caaaaactga ttccatcgaa aaaggtctgc cgattccggt agtaatcacc gtttacgctg
     1561 accgttcttt cactttcgtt accaagactc cgccggcagc agttctgctg aaaaaagcgg
     1621 ctggtatcaa gtctggttcc ggtaagccga acaaagacaa agtgggtaaa atttcccgcg
     1681 ctcagctgca ggaaatcgcg cagaccaaag ctgccgacat gactggtgcc gacattgaag
     1741 cgatgactcg ctccatcgaa ggtactgcac gttccatggg cctggtagtg gaggactaag
     1801 aaatggctaa actgaccaag cgcatgcgtg ttatccgcga gaaagttgat gcaaccaaac
     1861 agtacgacat caacgaagct atcgcactgc tgaaagagct ggcgactgct aaattcgtag
     1921 aaagcgtgga cgtagctgtt aacctcggca tcgacgctcg taaatctgac cagaacgtac
     1981 gtggtgcaac tgtactgccg cacggtactg gccgttccgt tcgcgtagcc gtatttaccc
     2041 aaggtgcaaa cgctgaagct gctaaagctg caggcgcaga actggtaggt atggaagatc
     2101 tggctgacca gatcaagaaa ggcgaaatga actttgacgt tgttattgct tctccggatg
     2161 caatgcgcgt tgttggccag ctgggccagg ttctgggtcc gcgcggcctg atgccaaacc
     2221 cgaaagtggg tactgtaacg ccgaacgttg ctgaagcggt taaaaacgct aaagctggcc
     2281 aggttcgtta ccgtaacgac aaaaacggca tcatccacac caccatcggt aaagtggact
     2341 ttgacgctga caaactgaaa gaaaacctgg aagctctgct ggttgcgctg aaaaaagcaa
     2401 aaccgactca ggcgaaaggc gtgtacatca agaaagttag catctccacc accatgggtg
     2461 caggtgttgc agttgaccag gctggcctga gcgcttctgt aaactaatgc ctttacgtgg
     2521 gcggtgattt tgtctacaat cttaccccca catataatgc ttaatgcaga cgtatatccg
     2581 agatattcgg gttgtggcaa ggcggcaact gagtgagtcg ccaggagcat agctaactat
     2641 gtgactggtg cgaatgaagg aagccaacgc cgccacaagt tgaatagcga cggatagaaa
     2701 agatttgttc gttggagcct ggcctatcca ggcctccgtc gaagaccgca ggagtttcgc
     2761 aagaaactta atcccctgcg tagacggtga cagaacgcta agattattct tttatattct
     2821 ggcttgtttc tgctcaccgt aattaagacg ctctctccgc ttggaggagt gaagtgagtt
     2881 ccagagattt tctctggcaa acatccagga gcaaagctaa tggctttaaa tcttcaagac
     2941 aaacaagcga ttgttgctga agtcagcgaa gtagccaaag gcgcgctgtc tgcagtagtt
     3001 gcggattccc gtggcgtaac tgtagataaa atgactgaac tgcgtaaagc aggtcgcgaa
     3061 gctggcgtat acatgcgtgt tgttcgtaac accctgctgc gccgtgctgt tgaaggtact
     3121 ccgttcgagt gcctgaaaga cgcgtttgtt ggtccgaccc tgattgcata ctctatggaa
     3181 cacccgggcg ctgctgctcg tctgttcaaa gagttcgcga aagcgaatgc aaaatttgag
     3241 gtcaaagccg ctgcctttga aggtgagctg atcccggcgt ctcagatcga ccgcctggca
     3301 actctgccga cctacgaaga agcaattgca cgcctgatgg caaccatgaa agaagcttcg
     3361 gctggtaaac tggttcgtac tctggctgct gtacgcgatg cgaaagaagc tgcttaatcg
     3421 cagttatctt tttaacgcat tcgcttacgt ataaacttat tctgatattc aggaacaatt
     3481 taaatgtcta tcactaaaga tcaaatcatt gaagcagttg cagctatgtc tgtaatggac
     3541 gttgtagaac tgatctctgc aatggaagaa aaattcggtg tttccgctgc tgctgctgta
     3601 gctgtagctg ctggcccggt tgaagctgct gaagaaaaaa ctgaattcga cgtaattctg
     3661 aaagctgctg gcgctaacaa agttgctgtt atcaaagcag tacgtggcgc aactggcctg
     3721 ggtctgaaag aagctaaaga cctggtagaa tctgcaccgg ctgctctgaa agaaggcgtg
     3781 agcaaagacg acgcagaagc actgaaaaaa tccctggaag aagctggcgc tgaagttgaa
     3841 gttaaataag ccaacccttc cggttgcagc ctgagaaatc aggctgatgg ctggtgactt
     3901 tttggtcacc agcctttttg cgctgtaagg cgccagtagc gtttcacact gtttgactac
     3961 tgctgtgcct ttcaatgctt gtttctatcg acgacttaat atactgcgac aggacgtccg
     4021 ttctgtgtaa atcgcaatga aatggtttaa gcgtgatagc aacaggcatt gcggaaagtg
     4081 ttccattttc cggtcaacaa aatagtgttg cacaaactgt ccgctcaatg gacagatggg
     4141 tcgacttgtc agcgagctga ggaaccctat ggtttactcc tataccgaga aaaaacgtat
     4201 tcgtaaggat tttggtaaac gtccacaagt tctggatgta ccttatctcc tttctatcca
     4261 gcttgactcg tttcagaaat ttatcgagca agatcctgaa gggcagtatg gtctggaagc
     4321 tgctttccgt tccgtattcc cgattcagag ctacagcggt aattccgagc tgcaatacgt
     4381 cagctaccgc cttggcgaac cggtgtttga cgtccaggaa tgtcaaatcc gtggcgtgac
     4441 ctattccgca ccgctgcgcg ttaaactgcg tctggtgatc tatgagcgcg aagcgccgga
     4501 aggcaccgta aaagacatta aagaacaaga agtctacatg ggcgaaattc cgctcatgac
     4561 agacaacggt acctttgtta tcaacggtac tgagcgtgtt atcgtttccc agctgcaccg
     4621 tagtccgggc gtcttctttg actccgacaa aggtaaaacc cactcttcgg gtaaagtgct
     4681 gtataacgcg cgcatcatcc cttaccgtgg ttcctggctg gacttcgaat tcgatccgaa
     4741 ggacaacctg ttcgtacgta tcgaccgtcg ccgtaaactg cctgcgacca tcattctgcg
     4801 cgccctgaac tacaccacag agcagatcct cgacctgttc tttgaaaaag ttatctttga
     4861 aatccgtgat aacaagctgc agatggagct ggtgccggaa cgcctgcgtg gtgaaactgc
     4921 atctttcgac atcgaagcta acggtaaagt gtacgtagaa aaaggccgcc gtatcactgc
     4981 gcgccacatt cgccagctgg aaaaagacga cgtcaaactg atcgaagtcc cggttgagta
     5041 catcgcaggt aaagtggttg ctaaagacta tattgatgag tctaccggcg agctgatctg
     5101 cgcagcgaac atggagctga gcctggatct gttggctaag ctgagccagt ctggtcacaa
     5161 gcgtattgaa acgctgttca ccaacgacct ggatcacggc ccgtatatct ctgaaacctt
     5221 acgtgtcgac ccaactaacg accgtctgag cgcactggta gaaatctacc gcatgatgcg
     5281 ccctggcgag ccgccgactc gtgaagcagc tgaaagcctg ttcgagaacc tgttcttctc
     5341 cgaagaccgt tatgacctgt ctgcggttgg tcgtatgaag ttcaaccgtt ctctgctgcg
     5401 cgaagaaatc gaaggttccg gtatcctgag caaagacgac atcattgatg ttatgaaaaa
     5461 gctcatcgat atccgtaacg gtaaaggcga agtcgatgat atcgaccacc tcggcaaccg
     5521 tcgtatccgt tctgttggcg aaatggcgga aaaccagttc cgcgttggcc tggtacgtgt
     5581 agagcgtgcg gtgaaagagc gtctgtctct gggcgatctg gataccctga tgcctcagga
     5641 catgatcaac gccaagccga tttccgcagc agtgaaagag ttcttcggtt ccagccagct
     5701 gtctcagttt atggaccaga acaacccgct gtctgagatt acgcacaaac gtcgtatctc
     5761 cgcactcggc ccgggtggtc tgacccgtga acgtgcaggc ttcgaagttc gagacgtaca
     5821 cccgactcat tacggtcgcg tatgtccaat cgaaacccct gaaggtccga acatcggtct
     5881 gatcaactct ttgtccgtgt acgcacagac taacgaatac ggcttccttg agactccgta
     5941 tcgtaaggtg accgacggtg ttgtaactga cgaaattcac tacctttctg ctatcgaaga
     6001 aggcaactac gttatcgccc aggcgaactc caacctggat gaagaaggcc acttcgtaga
     6061 agacctggta acttgccgta gcaaaggcga atccagcttg ttcagccgtg accaggttga
     6121 ctacatggac gtatccaccc agcaggtggt atccgtcggt gcgtccctga tcccgttcct
     6181 ggaacacgat gacgccaacc gtgcattgat gggtgcgaac atgcaacgtc aggccgttcc
     6241 gactctgcgc gctgataagc cgctggttgg tactggtatg gaacgtgctg ttgccgttga
     6301 ctccggtgta actgcggtag ctaaacgtgg tggtgtcgtt cagtacgtgg atgcttcccg
     6361 tatcgttatc aaagttaacg aagacgagat gtatccgggt gaagcaggta tcgacatcta
     6421 caacctgacc aaatacaccc gttctaacca gaacacctgt atcaaccaga tgccgtgtgt
     6481 gtctctgggt gaaccggttg aacgtggcga cgtgctggca gacggtccgt ccaccgacct
     6541 cggtgaactg gcgcttggtc agaacatgcg cgtagcgttc atgccgtgga atggttacaa
     6601 cttcgaagac tccatcctcg tatccgagcg tgttgttcag gaagaccgtt tcaccaccat
     6661 ccacattcag gaactggcgt gtgtgtcccg tgacaccaag ctggggccgg aagagatcac
     6721 cgctgacatc ccgaacgtgg gtgaagctgc gctctccaaa ctggatgaat ccggtatcgt
     6781 ttacattggt gcggaagtga ccggtggcga cattctggtt ggtaaggtaa cgccgaaagg
     6841 tgaaactcag ctgaccccag aagaaaaact gctgcgtgcg atcttcggtg agaaagcctc
     6901 tgacgttaaa gactcttctc tgcgcgtacc aaacggtgta tccggtacgg ttatcgacgt
     6961 tcaggtcttt actcgcgatg gcgtagaaaa agacaaacgt gcgctggaaa tcgaagaaat
     7021 gcagctcaaa caggcgaaga aagacctgtc tgaagaactg cagatcctcg aagcgggttt
     7081 gttcagccgt atccgtgctg tgctggtagc cggtggcgtt gaagctgaga agctcgacaa
     7141 actgccgcgc gatcgctggc tggagctggg cctgaccgac gaagagaaac aaaatcagct
     7201 ggaacagctg gctgagcagt atgacgaact gaaacacgag ttcgagaaga aactcgaagc
     7261 gaaacgccgc aaaatcaccc agggcgacga tctggcaccg ggcgtgctga agattgttaa
     7321 ggtgtatctg gcggttaaac gccgtatcca gcctggtgac aagatggcag gtcgtcacgg
     7381 taacaagggt gtaatttcta agatcaaccc gatcgaagat atgccttacg atgaaaacgg
     7441 tacgccggta gacatcgtac tgaacccgct gggcgtaccg tctcgtatga acatcggtca
     7501 gatcctcgaa acccacctgg gtatggctgc gaaaggtatc ggcgacaaga tcaacgccat
     7561 gctgaaacag cagcaagaag tcgcgaaact gcgcgaattc atccagcgtg cgtacgatct
     7621 gggcgctgac gttcgtcaga aagttgacct gagtaccttc agcgatgaag aagttatgcg
     7681 tctggctgaa aacctgcgca aaggtatgcc aatcgcaacg ccggtgttcg acggtgcgaa
     7741 agaagcagaa attaaagagc tgctgaaact tggcgacctg ccgacttccg gtcagatccg
     7801 cctgtacgat ggtcgcactg gtgaacagtt cgagcgtccg gtaaccgttg gttacatgta
     7861 catgctgaaa ctgaaccacc tggtcgacga caagatgcac gcgcgttcca ccggttctta
     7921 cagcctggtt actcagcagc cgctgggtgg taaggcacag ttcggtggtc agcgtttcgg
     7981 ggagatggaa gtgtgggcgc tggaagcata cggcgcagca tacaccctgc aggaaatgct
     8041 caccgttaag tctgatgacg tgaacggtcg taccaagatg tataaaaaca tcgtggacgg
     8101 caaccatcag atggagccgg gcatgccaga atccttcaac gtattgttga aagagattcg
     8161 ttcgctgggt atcaacatcg aactggaaga cgagtaattc tcgctcaaac aggtcactgc
     8221 tgtcggggta aaacccggca gcggattgtg ctaactccga cgggagcaaa tccgtgaaag
     8281 atttattaaa gtttctgaaa gcgcagacta aaaccgaaga gtttgatgcg atcaaaattg
     8341 ctctggcttc gccagacatg atccgttcat ggtctttcgg tgaagttaaa aagccggaaa
     8401 ccatcaacta ccgtacgttc aaaccagaac gtgacggcct tttctgcgcc cgtatctttg
     8461 ggccggtaaa agattacgag tgcctgtgcg gtaagtacaa gcgcctgaaa caccgtggcg
     8521 tcatctgtga gaagtgcggc gttgaagtga cccagactaa agtacgccgt gagcgtatgg
     8581 gccacatcga actggcttcc ccgactgcgc acatctggtt cctgaaatcg ctgccgtccc
     8641 gtatcggtct gctgctcgat atgccgctgc gcgatatcga acgcgtactg tactttgaat
     8701 cctatgtggt tatcgaaggc ggtatgacca acctggaacg tcagcagatc ctgactgaag
     8761 agcagtatct ggacgcgctg gaagagttcg gtgacgaatt cgacgcgaag atgggtgcgg
     8821 aagcaatcca ggctctgctg aagagcatgg atctggagca agagtgcgaa cagctgcgtg
     8881 aagagctgaa cgaaaccaac tccgaaacca agcgtaaaaa gctgaccaag cgtatcaaac
     8941 tgctggaagc gttcgttcag tctggtaaca aaccagagtg gatgatcctg accgttctgc
     9001 cggtactgcc gccagatctg cgtccactgg ttccgctgga tggtggtcgt ttcgcgactt
     9061 ctgacctgaa cgatctgtat cgtcgcgtca ttaaccgtaa caaccgtctg aaacgtctgc
     9121 tggatctggc tgcgccggac atcatcgtac gtaacgaaaa acgtatgctg caggaagcgg
     9181 tagacgccct gctggataac ggtcgtcgcg gtcgtgcgat caccggttct aacaagcgtc
     9241 ctctgaaatc tttggccgac atgatcaaag gtaaacaggg tcgtttccgt cagaacctgc
     9301 tcggtaagcg tgttgactac tccggtcgtt ctgtaatcac cgtaggtcca tacctgcgtc
     9361 tgcatcagtg cggtctgccg aagaaaatgg cgctggagct gttcaaaccg ttcatctacg
     9421 gcaagctgga actgcgtggt cttgctacca ccattaaagc tgcgaagaaa atggttgagc
     9481 gcgaagaagc tgtcgtttgg gatatcctgg acgaagttat ccgcgaacac ccggtactgc
     9541 tgaaccgtgc accgactctg caccgtctgg gtatccaggc atttgaaccg gtactgatcg
     9601 aaggtaaagc tatccagctg cacccgctgg tttgtgcggc atataacgcc gacttcgatg
     9661 gtgaccagat ggctgttcac gtaccgctga cgctggaagc ccagctggaa gcgcgtgcgc
     9721 tgatgatgtc taccaacaac atcctgtccc cggcgaacgg cgaaccaatc atcgttccgt
     9781 ctcaggacgt tgttctgggt ctgtactata tgacccgtga ctgtgttaac gccaaaggcg
     9841 aaggcatggt gctgactggc ccgaaagaag cagaacgtct gtatcgctct ggtctggctt
     9901 ctctgcatgc gcgcgttaaa gtgcgtatca ccgagtatga aaaagatgca aacggtgaat
     9961 tagtagcgaa aaccagcctg aaagacacga ctgttggccg tgccattctg tggatgattg
    10021 taccgaaagg tctgccttac tccatcgtca accaggcgct gggtaaaaaa gcaatctcca
    10081 aaatgctgaa cacctgctac cgcattctcg gtctgaaacc gaccgttatt tttgcggacc
    10141 agatcatgta caccggcttc gcctatgcag cgcgttctgg tgcatctgtt ggtatcgatg
    10201 acatggtcat cccggagaag aaacacgaaa tcatctccga ggcagaagca gaagttgctg
    10261 aaattcagga gcagttccag tctggtctgg taactgcggg cgaacgctac aacaaagtta
    10321 tcgatatctg ggctgcggcg aacgatcgtg tatccaaagc gatgatggat aacctgcaaa
    10381 ctgaaaccgt gattaaccgt gacggtcagg aagagaagca ggtttccttc aacagcatct
    10441 acatgatggc cgactccggt gcgcgtggtt ctgcggcaca gattcgtcag cttgctggta
    10501 tgcgtggtct gatggcgaag ccggatggct ccatcatcga aacgccaatc accgcgaact
    10561 tccgtgaagg tctgaacgta ctccagtact tcatctccac ccacggtgct cgtaaaggtc
    10621 tggcggatac cgcactgaaa actgcgaact ccggttacct gactcgtcgt ctggttgacg
    10681 tggcgcagga cctggtggtt accgaagacg attgtggtac ccatgaaggt atcatgatga
    10741 ctccggttat cgagggtggt gacgttaaag agccgctgcg cgatcgcgta ctgggtcgtg
    10801 taactgctga agacgttctg aagccgggta ctgctgatat cctcgttccg cgcaacacgc
    10861 tgctgcacga acagtggtgt gacctgctgg aagagaactc tgtcgacgcg gtaaaagtac
    10921 gttctgttgt atcttgtgac accgactttg gtgtatgtgc gcactgctac ggtcgtgacc
    10981 tggcgcgtgg ccacatcatc aacaaaggtg aagcaatcgg tgttatcgcg gcacagtcca
    11041 tcggtgaacc gggtacacag ctgaccatgc gtacgttcca catcggtggt gcggcatctc
    11101 gtgcggctgc tgaatccagc atccaggtga aaaacaaagg tagcatcaag ctcagcaacg
    11161 tgaagtcggt tgtgaactcc agcggtaaac tggttatcac ttcccgtaac accgaactga
    11221 aactgatcga cgaattcggt cgtaccaaag aaagctacaa agtgccttac ggtgcggtac
    11281 tggcgaaagg cgatggcgaa caggttgctg gcggcgaaac cgttgcaaac tgggacccgc
    11341 acaccatgcc ggttatcacc gaagtaagcg gttttgtacg ctttactgac atgatcgacg
    11401 gccagaccat tactcgtcag accgacgaac tgaccggtct gtcttcgctg gtggttctgg
    11461 attccgcaga acgtaccgca ggtggtaaag atctgcgtcc ggcactgaaa atcgttgatg
    11521 ctcagggtaa cgacgttctg atcccaggta ccgatatgcc tgcgcagtac ttcctgccgg
    11581 gtaaagcgat tgttcagctg gaagatggcg tacagatcag ctctggtgac accctggcgc
    11641 gtattccgca ggaatccggc ggtaccaagg acatcaccgg tggtctgccg cgcgttgcgg
    11701 acctgttcga agctcgtcgt ccgaaagagc cggcaatcct ggctgaaatc agcggtatcg
    11761 tttccttcgg taaagaaacc aaaggtaaac gtcgtctggt tatcaccccg gtagacggta
    11821 gcgatccgta cgaagagatg attccgaaat ggcgtcagct caacgtgttc gaaggtgaac
    11881 gtgtagaacg tggtgacgta atttccgacg gtccggaagc gccgcacgac attctgcgtc
    11941 tgcgtggtgt tcatgctgtg actcgttaca tcgttaacga agtacaggac gtataccgtc
    12001 tgcagggcgt taagattaac gataaacaca tcgaagttat cgttcgtcag atgctgcgta
    12061 aagctaccat cgttaacgcg ggcagctccg acttcctgga aggcgaacag gttgaatact
    12121 ctcgcgtcaa gatcgcaaac cgcgaactgg aagcgaacgg caaagtgggt gcaacttact
    12181 cccgcgatct gctgggtatc accaaagcgt ctctggcaac cgagtccttc atctccgcgg
    12241 catcgttcca ggagaccact cgcgtgctga ccgaagcagc cgttgcgggc aaacgcgacg
    12301 aactgcgcgg cctgaaagag aacgttatcg tgggtcgtct gatcccggca ggtaccggtt
    12361 acgcgtacca ccaggatcgt atgcgtcgcc gtgctgcggg tgaagctccg gctgcaccgc
    12421 aggtgactgc agaagacgca tctgccagcc tggcagaact gctgaacgca ggtctgggcg
    12481 gttctgataa cgagtaatcg ttaatccgca aataacgtaa aaacccgctt cggcgggttt
    12541 ttttatgggt gttattcaga gggcaaattc ttcacggaaa atattaagaa aggcagggcg
    12601 aaacgaggaa gaagcttttc gccctggaca acattcctgc tgacggcact accataaaaa
    12661 aacacaacgc tttcaacaac cttgcgtttt tcatcttccg tagcggctcg gaataaattt
    12721 acgttcttac attccgtctc atagtctttg cgaggcgcgt cccagatagc tgtcccagtc
    12781 tttccatacc ggctgcaaac cctgagcggt taacgcggca gcaaccgctt ccggtctgcg
    12841 atcgtcgtgc ggtgagaact gttccaactc ggggtgatta tcggcatagc cacctggctg
    12901 cgttttcgag aaggcgctga cgttattgat cgccagcgga ataacgcgat cgcgaaacca
    12961 cggtgattcc cgcgtggaga gtgacagttc aatctccggt gcaagcagtc ggaaggcgca
    13021 gatggtttgc actaactggc gttcatccat aatcgacgca ggctcaatgc cgccagtaca
    13081 cgggcgcagg cgcggaaagg agacagagta acggctttgc cagtaatgct gttgcagcca
    13141 tagcaaatgt tcggcaacca tatagcagtc aacgcgccag ttgtcggaaa ggccaattag
    13201 cgcgccgagg cctatcttat caatccccgc acgccccagc cgatccggcg tttccagccg
    13261 ccagaagaag tcctgttttt tgcctttcaa atgatggcgg gtataagtcg cctcgtgata
    13321 tgtctcctga taaaccatca ccccatccag accaagttgc tttaactcgg cgtattccgt
    13381 ctccgccagc ggttgcactt ccatttgtag tgaagagaac tgttcacgca gggcggggag
    13441 atgacgacga aagtaatcca tccccacttt cgcctgatgt tcaccagtga ctaatagcag
    13501 atgttcaaag cccatctccc gtatagcggc gctttccctg gcaatatccg cttcatccag
    13561 cgttttgcgc ttgatgcgat tactcatgga aaatccgcag tacgtgcagt cgttagcgca
    13621 aagattggaa agataaagcg ggacgtagaa actaactgtg ttgccaaatc gctgacgggt
    13681 cagacgctgc gcccgttggg ccagtggttc cagatagcca ctggcggctg gcgataacag
    13741 cgccatcata tcgtcgcggg tgagttgcga ggcatttagc gcccgttcta cgtcagcagc
    13801 cgttttgccg ttgatacgca ggcggatgtc gtcccagtcc agttgtcgcc agcgatcgct
    13861 gaaggttttc atgccgatgc ctccagaaat ccagtcagcg ggctggtggc atgagcaaaa
    13921 tgactgcggc tgcccggtcc ggactgacgt gccagcaggc ctgcttctac cgccagacga
    13981 aatgccttcg ccatgttgac gggatcgtcc gctacggcaa tcgccgtatt cactaacacc
    14041 gcgtcggccc ccatttccag ggcctgcgcg gcatggctgg gaacgccgat gccagcatcg
    14101 acaaccaccg gaactgtggc ctgctggata ataatctcca gcatggcacg ggtttccagt
    14161 ccctgattcg agccaatcgg cgcgccgagc ggcatcaccg ctgcacagcc gacttcttcc
    14221 agacgtttac acaataccgg atcggccccg cagtaaggca gcacgacaaa tccctgttgt
    14281 accagcattt cggcggcttt cagagtttcg atgggatcgg gcaacagcca gcgggcgtca
    14341 gggtgaatct ctaattttaa ccagtttgtg cctaacgctt cacgtgccag atgggcggcg
    14401 aaaatggctt cttccgctgt tttcgccccg gaagtatttg gcagcagggt cacacccgcc
    14461 gcgataagcg gttcgaggat agcgtcgttg tgctggcgca agtcgacacg tttcatcgcc
    14521 agtgtcacca gctggctgcc ggaagcgcgg atcgcctcca ccatcagttg tgaagaagcg
    14581 aatttccctg tgccggtaaa cagatgtgaa tcaaacgttt tgtccgcaat acgtaacatc
    14641 tcaaccccct gcaataacct gaaaaagcag gatctggtcg ccatcctgca cgatatgttg
    14701 cgtccactgc tcacgcggga cgatttgctg attaatcgcc agagccgcgc ccgcttgtcg
    14761 ttggtcgagt tgctccagta gttcgtgaac agtttgcccg gcggcgcact gcatcggttg
    14821 atcgttaaac aggatctgca ttgcttcctc cgcataccgg gcaaccactg gcgcggcgca
    14881 acgccaggct gcgccactgg ctcgatttac cgtcgaacag tcggagttct cccgcaggtg
    14941 tctctatacc gcttaataac ttaatggctt ccagtgcctg caaagtgccc ataaccccga
    15001 ccaccgggcc aaccacgccc gccgtgcggc agttgcgttc tggctcctgg ttatctggcc
    15061 acaggcagcg gtaacacccc tgctcccagg gcggcgtcag taccatcaac tgaccgccaa
    15121 atccgaccgc gctggcggtg ataagcggcg tgttgagtac cacgcaggcg gcattaatct
    15181 cctggcgagt cgccatattg tcggtacagt cgagcaccac atcggcccgt gcaaccgcat
    15241 cttttaacgc ctcacccgtt aaccgttgtt gtaatgctgt cagttgaata tcgggattca
    15301 actgtgtcag tcgctgatgg ctgacctgcg atttcgggcg atcgatatct tcagtggtaa
    15361 agaggatttg tcgttgcaga ttgcttaaat gcacatcgtc gtcatctgcc agtaccagcg
    15421 tcccgacgcc agcccccgcc aggtatagcg cagcaggtgt acccagcccg ccaagaccaa
    15481 taatcagcac ctggctgtcg agcagttttt gctgcccgtc cagagcgata tcgtcgagca
    15541 gaatttggcg gctataacgc ataaagtcac ggtcattcat cgccaactcc tgcaatttcc
    15601 agcaactgtg ccgttgccaa acgccagtct gcggcttgag taatggcgct gacgacggcg
    15661 atactgccga cacccgttgc tatcaccgca ggcgcgcgcg ccagactgat accgccaatc
    15721 gccacggtgg gataatccgc cagtcgctca acatgccgtg ccagctgttc cagcccctgc
    15781 ggtgcagaag gcatctgttt ggtttgcgtc gggaacacat gtcccagcgc gatataagag
    15841 gggcgtgctg ccagcgcgac gtcgatttcc atatcgtcat gtgtcgaaac gcccagccgc
    15901 aggcctgccg cgcggatggc attgagatcg gtggcttgca aatcttcctg ccccaaatgg
    15961 acgccatacg cctgatgctt gatcgccagc cgccagtaat cgttgataaa caatcgcgcg
    16021 ttatagcggc ggcccagcgc aattgccgcc acgacatcgg cttccacctc ttcattgcgc
    16081 cgatctttga tgcgtagctg gagagtacgt acgcctgcat ccaacagacg ttcgatccac
    16141 tgtacgctgt ccaccaccgg gtacagtcct aaacgaaaag gtacaggagg aaaatcaggc
    16201 tgatacatca cgcttcctcc ttacgcaggt agatttctcc gcctctggcg cggaagttct
    16261 ccgacatatc cgccattccc acttcaatgg tttgcgcggc ggcgtaatca cgtacttcct
    16321 ggctgatttt catcgagcag aatttcggcc cacacatgga gcaaaaatgg gcgactttac
    16381 ctgactcttg cggcagggtt tcatcgtgat aagcgcgggc ggtaaacggg tcgagggcca
    16441 gattaaactg gtcttcccag cgaaattcga agcgggcttt cgacatggcg ttatcgcgaa
    16501 tttgcgcgcc tggatgccct ttcgccaggt cagcggcgtg ggcggcaatc ttataggtga
    16561 taagcccctg cttaacatct tctttattag gcagacccag atgctctttt ggcgttacgt
    16621 aacagagcat cgcgcagcca aaccagccaa tcatcgccgc accaatcccc gacgtgaagt
    16681 ggtcatagcc cggcgcaata tcggtagtta gcggccccag agtgtaaaac ggcgcttcgt
    16741 ggcagtgctc taactcctcg gtcatattgc ggcggatcat ctgcatcggc acgtggcctg
    16801 ggccttcaat catcacctgc acgtcatatt cccaggcaat tttggtcagt tcgcccagcg
    16861 tatgcagctc ggcaaactgc gcttcatcgt tggcgtcctg aatagaaccg gggcgcagac
    16921 cgtcgcccag cgacagcgaa acgtcataag cggcacagat ttcacaaatt tcgcggaagt
    16981 gttgatagag gaaattttcc tgatgatggg agaggcacca tttcgccata atcgaaccgc
    17041 cgcgagagac gataccggtc aggcgtttcg cggtcatcgg cacatagcgc agcagtacgc
    17101 ccgcatggat agtgaagtaa tccacacctt gctcggcctg ttccagcagc gtgtcgcgga
    17161 acgcttccca ggtaagatct tcggcgatcc cgttaacctt ctccagcgcc tggtagatcg
    17221 gcactgtacc gatcggcacc gggctgttac gcaaaatcca ctcgcgggtt tcgtgaatat
    17281 agcgaccggt ggagagatcc atcaccgtat ccgctcccca gcgcgtggac cataccagct
    17341 tttccacttc ttcttcgatg gaagaggtga ccgccgagtt gccgatattg gcgttaactt
    17401 ttaccaggaa attgcgacca ataatcatcg gctccgattc cggatgatta atgttggccg
    17461 ggataatcgc acgtccggca gcaacttcat cacggacaaa ttccgcagtg atattttccg
    17521 gcagatgtgc gccaaagctc attcccggat gctggtggcg taaaacctcg ctacggatgc
    17581 gctcgcggcc catattctcg cggatggcga tgaattccat ttccggcgtg atgatgccct
    17641 ggcgggcgta gtgcagttgg gtgacacggc gtcctgtttt ggcgcgtttt ggcgttaata
    17701 agccgctaaa acgtagctcg tcgagaccat catctgccag ccgcgttctg gtgtaatcgg
    17761 aactgcgcac ggtaagttct tcggtatcgc cgcgcgcatc gatccacggc tggcgtagtt
    17821 ttgccagccc ttgctgcacg ttaatggcga tctgcggatc accatacggg ccggaggtgt
    17881 cgtagaccgg aatcgcttcg ttttcttcgt actgcggctg ttctttgcta ccgccaatta
    17941 gcgtcgggct aagctggatc tcacgcatcg gcacgcgcac gccggggtgt gtgccagtga
    18001 tataaatgcg ttttgagttg ggaaaggcgg tgccttccag ggtgtcgata aaatgttggg
    18061 cccgggcgcg ttgttcgcgg cgggtcagtt ttgttgcaga catagctcat tccaaaaagt
    18121 caaggacgtg gcttgtcaga cgacggatga agcaagagac gatcgccgca aaggcgatgc
    18181 gatagcagat taactcttgt tcccttcgca ggtattagcc tgatcaggtt ccgcggatcc
    18241 cgaataaacg gtctcagcca gttaaggcac tccgacaaga aattagcccc cacaaacggg
    18301 gcgttgaatg taaattacgc gttaacagcg cagaactcaa gcaggatgtt tgacgcgggc
    18361 ggcgtcaagc accagcagaa tcagcttatc ttccagcaca aagcgcgctt ccagcgcctc
    18421 gccgatgtca gataaaacct gttgaaactc aaggtaatta tcatgatcga tggcggtttc
    18481 caggctggaa tcgtagtaat ccataatctg ttgggtattg gcttcgagtt gcggccaaat
    18541 cttcgcggcg cgcgcgagtt gcccgttgcc ttccagctta tgaagaatgc gctcataaat
    18601 actgaaatgt ccggcagaca agtaatcgac caggctctga caaaaatcat caagggcttt
    18661 ttcgtttagc ctcatgtacg attctttgcc aggcttaatg ccaaccagat tgtagtaagc
    18721 cacgagcaga tgcttacgta catgtagcca gcgatcaacc agtttgttac ttcctctgac
    18781 gcgctccgtc aggttatcga gctggttaag catgattgac tccgcaagtt tgtattcaaa
    18841 aactgctcag tgagaaatgt aaaaaccatg ttaaacatgc cagtgatgca aaggtagtgc
    18901 aagagctatg gatcgtataa ttgaaaaatt agatcacggc tggtgggtcg tcagccatga
    18961 acaaaaatta tggttgccga agggagaatt gccatatggc gaagcggcaa atttcgatct
    19021 tgtgggtcag cgcgcactac aaatcggcga atggcagggg gaacctgttt ggttagtaca
    19081 acagcagcgg cgtcacgata tggggtcggt acgtcaggtc attgatctcg atgttgggct
    19141 gtttcaactg gccggacgag gcgtacaact ggcggagttt taccgatcgc ataaatactg
    19201 tggttactgc gggcatgaaa tgtatccgag caaaaccgaa tgggcgatgc tgtgcagcca
    19261 ttgccgtgag cgttactacc cgcaaatcgc cccctgcatt attgttgcca tccgtcgcga
    19321 tgattcgatc ctcctcgccc agcatacccg ccatcgtaac ggtgtccata cagtacttgc
    19381 cggattcgtc gaagtgggcg aaaccctcga gcaggcagtc gcgcgggaag tgatggaaga
    19441 gagcggaatt aaagttaaaa acttgcgtta cgtgacttct cagccgtggc cgtttcctca
    19501 gtctttaatg accgcgttta tggcggaata tgacagcggc gacatcgtga tcgacccgaa
    19561 agaattgctc gaggcgaact ggtatcgcta tgacgatttg ccgttactcc cgccgcccgg
    19621 caccgtagcg cgccgtctga tagaagatac tgtggcgatg tgtcgggcag agtatgagtg
    19681 atgatacact gaccgcctga cgcactaagg aacagccaaa atgaccgaac ttaaaaacga
    19741 tcgttatctg cgggcgctgc tgcgccagcc cgttgatgtc actccagtat ggatgatgcg
    19801 ccaggcgggt cgctatctac cggaatataa agccacgcgc gcccaggcgg gcgattttat
    19861 gtcgctgtgc aaaaacgccg agctggcgtg cgaagtgact ttgcaaccgc tgcgtcgcta
    19921 cccgctggat gcggcgatcc tcttttccga tatcctcacc gtgccggacg cgatggggtt
    19981 agggctctat tttgaagccg gagaaggtcc gcgttttacc tcgccagtca cctgcaaagc
    20041 cgacgtcgat aaactgccaa ttccggaccc ggaagatgag ctgggttacg tgatgaacgc
    20101 ggtgcgtacc attcgtcgcg aactgaaagg cgaagtgccg ctgattggtt tttccggcag
    20161 cccgtggacg ctggcgacct acatggtgga aggcggcagc agcaaagcgt tcaccgtgat
    20221 caaaaaaatg atgtatgccg atccgcaggc gctgcacgct ctactcgata aactggcgaa
    20281 aagcgtcact ttgtatctga atgcgcagat taaagccggt gctcaggcag tgatgatttt
    20341 cgacacctgg ggcggtgtgc ttaccgggcg cgattatcaa cagttctcgc tctattacat
    20401 gcataaaatt gttgatggtt tactgcgtga aaacgacggt cgccgcgtgc cagtcacgct
    20461 gtttaccaaa ggcggcggac agtggctgga agcgatggca gaaaccggtt gcgatgcgtt
    20521 gggcctcgac tggacaacgg atatcgccga tgcgcgccgc cgtgtgggca ataaagtcgc
    20581 gttgcagggt aatatggatc cgtcgatgct gtacgcgccg cctgcccgca ttgaagaaga
    20641 agtagcgact atacttgcag gtttcggtca cggcgaaggt catgtcttta accttggtca
    20701 cggcattcat caggatgtgc cgccagaaca tgctggcgtg ttcgtggagg cagtgcatcg
    20761 actgtctgaa caatatcacc gctaaggagt gattatggat ctcgcgtcat tacgcgctca
    20821 acaaatcgaa ctggcttctt ctgtgatccg cgaggatcga ctcgataaag atccaccgga
    20881 tctgatcgcc ggagccgatg tcgggtttga gcagggcgga gaagtgacgc gagcggcgat
    20941 ggtgctgctg aaatatccct cgcttgagct ggtggagtat aaagttgccc gcatcgccac
    21001 caccatgcct tacattccag gttttctttc cttccgcgaa tatcctgcgc tgctggcagc
    21061 gtgggagatg ctgtcgcaaa agccggattt agtgtttgtc gatggtcatg ggatctcgca
    21121 tcctcgccgt cttggcgtcg ccagccattt tggtttgatg gtggatgtgc caacgatcgg
    21181 cgtggcgaaa aaacggctct gcggtaaatt cgaaccgctc tccagcgaac cgggcgcgct
    21241 ggccccactg atggataaag gcgagcagct ggcctgggtc tggcgcagca aagcgcgctg
    21301 taacccgttg tttatcgcta ccggccatcg ggtcagcgtg gacagcgcgc tggcgtgggt
    21361 acaacgctgc atgaaaggct atcgtctgcc ggagccaacg cgctgggcgg acgcggtggc
    21421 ctcggaacgt ccggcgttcg tgcgctatac agcaaatcag ccctaattca ggtaaactgc
    21481 ggataatttc cgtatttgag aactcaacat gttacaaaac ccaattcatc tgcgtctgga
    21541 gcgcctggaa agctggcagc atgtcacttt catggcttgc ttatgcgaac gcatgtaccc
    21601 caattacgcc atgttctgcc agcaaaccgg ttttggtgat gggcaaattt accgtcgtat
    21661 tctcgatctc atctgggaaa cgctgaccgt taaagacgca aaagtaaatt tcgacagcca
    21721 actggagaaa tttgaagaag cgattccttc agccgacgat ttcgatctgt acggcgttta
    21781 tccggcaatc gatgcctgcg tggcgttaag tgaactggtc cattcgcgtt tgagtggtga
    21841 aacgctcgaa cacgcggtgg aagtgagtaa gacctccatc acgaccgttg cgatgctgga
    21901 aatgactcag gctggtcgcg aaatgagcga tgaagagctt aaagaaaacc cagctgtaga
    21961 gcaagaatgg gacattcagt gggaaatatt ccgactttta gctgagtgcg aagaacgcga
    22021 tatcgagctg ataaaaggcc ttagggcaga cctgcgtgag gcgggtgaga gcaatattgg
    22081 tataattttt cagcaataag accagaaaac gtgatttaac gcctgatttg tcgtacctgg
    22141 agtcttccct ttcgcccccc gtctggtcta catttggggg gcgaaaaaaa gtggctatcg
    22201 gtgcgtgtat gcaggagagt gctattctgg catttccgtc gcactcgatg cttagcaagc
    22261 gataaacaca ttgtaaggat aacttatgaa caagactcaa ctgattgatg taattgcaga
    22321 gaaagcagaa ctgtccaaaa cccaggctaa agctgctctg gagtccactc tggctgcaat
    22381 tactgagtct ctgaaagaag gcgatgctgt acaactggtt ggtttcggta ccttcaaagt
    22441 gaaccaccgc gctgagcgta ctggccgcaa cccgcagacc ggtaaagaaa tcaaaattgc
    22501 cgcagctaac gtaccggcat ttgtttctgg caaggcactg aaagacgcag ttaagtaaga
    22561 ttgcgtggca gtgaacagtt ttaacgaagg ggtggtttca ccccttttgt ctttctggcg
    22621 tcgatcattg atgctggctg gcgctctgct tctcactgcc tgtagtcata actcttcact
    22681 tcctcccttt accgccagtg gatttgctga agaccagggc gcggtacgca tctggcgaaa
    22741 agacagcggc gataatgtgc atctgcttgc cgtgtttagc ccgtggcgca gtggcgatac
    22801 cacgacgcga gagtatcgct ggcagggcga taacctcacg ctcatcaata tcaatgttta
    22861 cagcaaaccg ccggtgaata ttcgcgcgcg ttttgacgat cgcggtgatc tgagctttat
    22921 gcaacgtgaa tccgatgggg aaaagcagca gctttctaac gaccaaatcg atttataccg
    22981 ttatcgtgct gatcagatcc gccagattag cgatgcctta cgtcagggga gagtcgtgct
    23041 gcgccagggg cgctggcatg cgatggaaca gaccgtgacc acctgcgaag ggcaaaccat
    23101 taaacctgat ttagattcgc aggcgatagc gcatatcgag cgccgccaga gccgctcttc
    23161 tgttgatgtc agcgtggcat ggctggaagc gcccgaaggt tcgcaattac tgttagtggc
    23221 aaactctgat ttctgtcgct ggcaacccaa cgagaaaacg ttctgattta ccagtggccc
    23281 atacccatat gaccgccacc accgcagccg ccgtagccca tgcccattcc ggctccgcgc
    23341 ggaatacccg cttcagccat cgcgatatct cgtttcaccc gtaactcatc taacgactga
    23401 cgcaaattct ccatctcttt ggcgaccgcg ttaattttgc tgctatccgg tgggttcgcg
    23461 gctaacaggg cattgtattc ataacgcttc gtcaccagtt gctgttgcag tgcgctgctt
    23521 tgagcgtaaa agtcattatg gattttctgc cacgctgtct gttgttcgct ggtcaaaggc
    23581 gcggcatttt gctgccacat accgtgtccg ccgtgagcaa atgcagatgt cgatcccatc
    23641 gccattgctg aaagcgccat cattaccagg gcaattttcg tgttccgttt catggttaat
    23701 cctccagtgg ttgtcttact tcgggtattg catcttccgt gccaacgatg aaacgctgat
    23761 atgacgggta atctggcatg ataaacgagt aaaaatgact cgcctgctgc gggtagcgag
    23821 tcatttttac tcattgaaac ttaagccttt gtgttacagc gcagggtaag cgctgataaa
    23881 agatggcatg atttctgctg tcagaaaggg atgagcaggc aaagaagaag atgcgtttta
    23941 tgcaacgttc taaagactcc ttagctaaat ggttaagcgc gatcctcccc gtggtcattg
    24001 ttgggctggt agggttgttt gcggtgacgg tgattcgcga ttatgggcgc gagactgccg
    24061 ccgccagaca aacgctgctg gaaaaaggca gtgtacttat tcgcgctctt gaatccggct
    24121 cgcgcgtcgg catggggatg cgcatgcatc atgcgcagca gcaggcctta ctggaagaaa
    24181 tggccgggca gcctggagta cgttggtttg cggtcacgga tgaacaagga acaatcgtga
    24241 tgcatagcaa ctccggcatg gtgggaaaac agctttattc cccgcaggaa atgcagcagt
    24301 tacatccggg agatgaagaa gcgtggcggc ggatcgatag cgcagacggc gagcctgttc
    24361 tggaaattta tcgccagttt caaccgatgt ttgctgctgg aatgcaccgg atgcgccata
    24421 tgcagcagta tgccgcgaca ccacaagcaa ttttcatcgc tttcgacgcc agtaatattg
    24481 tgagtgccga agatcgtgag cagagaaaca ccctgattat cctcttcgcc ctggcgacgg
    24541 tcttgctggc aagcgtattg tcattcttct ggtatcgccg ctatttgcgc tcgcgccagc
    24601 ttctacaaga tgaaatgaag cgcaaagaga agctggtggc gctggggcat cttgcggcag
    24661 gcgttgccca cgaaatccgt aacccacttt cctcgattaa aggactggcg aaatactttg
    24721 ccgagcgcgc gcctgcaggg ggagaagcgc atcaactggc gcaggtgatg gcgaaagagg
    24781 ccgaccgttt aaaccgcgtg gtaagcgagc tgctggaact ggttaagcca acgcatctgg
    24841 ctttgcaggc ggtggatctc aacacgctga ttaaccactc attacagctg gtaagccagg
    24901 atgcgaacag ccgggagatc cagttacgct ttaccgccaa cgacacatta ccggaaattc
    24961 aggccgatcc ggacaggctg actcaggtcc tgttgaatct ctatctcaat gctattcagg
    25021 cgattggtca gcatggcgtg attagcgtga cggccagcga aagcggcgcg ggcgtgaaaa
    25081 tcagcgttac cgacagcggt aagggaattg cggcagatca gcttgatgcc atcttcactc
    25141 cgtacttcac cactaaagcc gaaggcaccg gactggggct ggcggtcgtg cataatattg
    25201 ttgaacaaca cggtggtaca attcaggtcg caagccagga gggaaaaggc gcaacgttca
    25261 ccctctggct tccggtcact attacgcgta agggcccaca aggatgacgc acgataatat
    25321 cgatattctg gtggtggatg atgacattag ccactgcact attttgcagg ctttactgcg
    25381 cggctggggc tataacgtcg cgctggcgaa cagcgggcga caggcgctgg agcaggtgcg
    25441 ggaacaggtt tttgatcttg tgctttgcga tgtgcgaatg gcggagatgg acggcattgc
    25501 cacgctaaaa gagatcaaag cgttaaatcc ggcgattccg gtgctgatta tgaccgcata
    25561 ctccagcgtc gagacggcgg tagaagcgtt gaaaaccggg gcgctggatt atctcatcaa
    25621 gccgctggat ttcgataacc tgcaggcgac gctggaaaag gcgctcgcgc atacgcacag
    25681 tattgacgct gaaacgcctg cggtgtctgc cagccagttc ggtatggtcg gtaaaagccc
    25741 ggcgatgcaa cacctgctca gtgaaatcgc cctcgtcgcg ccatcggaag ccacggtgct
    25801 gatccacggc gattccggca ccggtaaaga gctggtcgcc agggcgattc acgccagtag
    25861 cgcacgtagc gaaaaaccgc tggtaacgct caactgtgcg gcactcaacg aatccttgct
    25921 ggaatctgaa ttgttcggtc acgaaaaagg ggcgttcact ggagccgata gacgccggga
    25981 agggcgcttt gttgaggcgg acggcggcac gctgtttctc gatgaaattg gcgatatctc
    26041 gccgatgatg caggtgcgtc tgctgcgtgc gattcaggag cgcgaagttc agcgtgtcgg
    26101 tagcaaccag actatctcgg ttgatgtccg gttgattgcg gcgacccatc gcgatcttgc
    26161 cgcagaggtg aatgccgggc gttttcgcca ggatctctac tatcgcctga atgtggtggc
    26221 gattgaagta ccatcgctgc ggcaacggcg ggaagatatc cctctgctgg ctgtccattt
    26281 tcttcagcgc tttgccgagc gtaatcgcaa ggcggtaaaa ggctttacgc cacaggcgat
    26341 ggatttgctg atccactacg actggccggg aaatattcgt gaactggaaa acgcggtgga
    26401 acgggcggtg gtgctgctga ccggggaata tatttccgaa cgcgagctgc cgctggcgat
    26461 tgccggtacg ccgatcccac tggggcaaag tcaggatatt cagccgctgg tggaagtcga
    26521 aaaagaagtg attctggcgg cactggagaa aacgggcggc aacaaaaccg aagccgcccg
    26581 tcagttaggg atcacgcgca aaacgctatt ggcaaaactg tcgcgttagt tctgctcgcg
    26641 ttcgatagcg cgccagccga tatctttccg gcagaagcag tcgtcccagt gaatatcggt
    26701 cattaaggca taagcgcgtt tctgcgcttc tgccacggta tgacccagcg cggtgacgca
    26761 cagtacgcgc ccgccgctgg tcactacctg ctcgttatcc gccagtttcg tgcccgcgtg
    26821 gaacactttg ccgcctgcca cttcttccag cggcaggccg tggatcacat cgccggtgcg
    26881 gtaatcgccc ggatatccac ccgcagccat caccacgccg agagaagcac gctcatccca
    26941 ctctgacgtt ttctcgtcca gtttgccttc acaggccgcc aggcagagct caaccagatc
    27001 ggacttcatg cgcagcataa tcggctgggt ttctggatcg ccaaagcggc agttaaattc
    27061 gataaccttc ggattgccct gtttgtcgat catcaggccc gcgtagagaa aaccggtgta
    27121 ggtgttgcct tccgccgcca tgcctttcac ggttggccag atgatacgtt ccatggtgcg
    27181 ctgatgaact tcatcggtta ctaccggcgc gggggagtaa gcgcccatcc cgcccgtgtt
    27241 tggtccggta tctttatcgc ctacgcgttt gtgatcctgg ctggtggcca tcggcagcac
    27301 atgctcgccg tctaccatca cgataaagct cgcttcttcg ccgtcgagga actcttcgat
    27361 aacgatgcga tgacccgcgt cgccaaaagc gttgcccgcc agcatatcgt gaacagccgc
    27421 ttcggcttct tccagcgtca tcgccacgat aacgcctttc ccggctgcca gaccgtccgc
    27481 tttaatgacg atcggcgcac ctttctcacg cagatacgcc agcgcaggtt ctacctcggt
    27541 gaagttctgg tattccgctg tagggatctt atggcgggcc aggaaatctt tggtaaacgc
    27601 tttcgaacct tccagttgcg cggcacctgc ggttgggccg aagattttca tcccggcggc
    27661 gcggaaggta tcgaccacgc ctttcaccag cggcgcttcc gggccgacga tggtcagatc
    27721 aatcttttcg ttttgtgcga aatccaacag cgccgggata tcggtcacgc cgatagcaac
    27781 gttttgcagc gcgggttcca gcgcagtgcc tgcattaccc ggagcaacaa aaacagtttc
    27841 aaccagcggc gactgcgccg ctttccaggc cagcgcgtgc tcgcgcccgc cgttaccaat
    27901 cactaatact ttcatctatt gctccattaa tggcggaagt ggcgcatgtc ggtgaagagc
    27961 atcgcaatac cgtgctcgtc ggcggcggca atcacttcgt catcacggat agaaccgcca
    28021 ggctggatta cgcaggtcac gcctgcagcg gcggcggcat caataccgtc gcggaacggg
    28081 aagaacgcgt cagaagccat cgaggaacct ttcacttcca ggccttcatc ggcagcttta
    28141 ataccggcga ttttcgcgga gtacacgcgg ctcatctggc ccgcgccaat cccgatggtc
    28201 atattgtttt tggcatagac gatagcgttg gatttcacga acttcgccac tttccagcag
    28261 aacagcgcat cacgcagttc ctgttcggtc ggctgacgtt gggtgacgac gcgcagttct
    28321 tccgcaccga ccatgcccag gtcacgatcc tgaaccagca gaccgccgtt cacgcgtttg
    28381 aaatcaagac ccggaacacg ctcgccccac tgaccgcagg tcagaacgcg tacgttctgc
    28441 ttggcggcgg tgattttcag ggcttcttcg ctggcggaag gcgcaataat cacttcgaca
    28501 aactgacgag aaatgatggc ctgtgcggtt tccgcatcca gctcgcggtt aaaggcaatg
    28561 atgccgccga atgcggaggt tgggtcggtt ttgtacgcgc gatcgtaagc atcaagaatg
    28621 gaattgccga tagccacgcc gcaagggttg gcgtgcttca caatcacaca tgccggctcg
    28681 gcgaactctt tcacgcactc cagcgccgca tcggtatcgg cgatgttgtt ataagagagg
    28741 gctttaccct gaacctgggt tgcggtagca acggaggctt ctttcacatt ctcttctata
    28801 tagaaggcag cctgctggtg gctgttctcg ccgtaacgca tatcctgctt cttaatgaag
    28861 ttcaggttca gcgtgcgtgg gaagcgaccg gcggcttctt tgctttcacc gtggtaagcc
    28921 ggaaccatgc tgccgaagta gttggcaatc atgctgtcgt aggcggcagt gtgttcgaag
    28981 gctttgatgg cgaggtcgaa acgggttgca agcgtcagcg atccttcgtt gtcatccatc
    29041 tctttaataa tggcgtcgta gtcgctgctc ttcaccacga ttgcgacatc tttatggttc
    29101 ttagcggcgg agcgcaccat ggtcgggccg ccgatatcga tgttctcaac cgcatcttcc
    29161 agcgagcagc cttcacgagc aacagtctgg gcgaacggat acaggttaac aaccaccata
    29221 tcgataggct ggatctgatg ttcttccata atggcatcgt cctggccgcg acggcccaga
    29281 atgccaccat gtactttcgg atgcagggtc ttcacgcgtc catccatcat ctccgggaaa
    29341 ccggtgtaat cggaaacttc ggttaccggc agaccttttt ctgctaacag acgggcagtg
    29401 ccccctgtag acagcagctc cacaccgcgt gcggaaagtg cctgggcgaa ttcgacgata
    29461 ccggctttgt cagaaacact gagcagagcg cggcggactg gacgacgttg ttgcatggta
    29521 aatcccctgg atttgactat tacagagagc gttagctgaa tttttcgcga aaaactcagc
    29581 taacgcccct aacggggcat ccttattttt cgcccgcatt gtaacgaaaa cgtttgcgca
    29641 acgctcgcga atttttctct ttcaatggtg atcacaattt tgacagtggt taccgtggga
    29701 aaaatacaga aattacattg atgattgtgg ataactctgt gcgtaaaaag gtataaagcg
    29761 gggttttgct ggggaatgca gcagtcagtc atttttctgc aatttttcta ttgcggcctg
    29821 cggagaactc cctataatgc gcctccatcg acacggcgga tgtgaatcac ttcacacaaa
    29881 cagccggttc ggttgaagag aaaaatcctg aaattcaggg ttgactctga aagaggaaag
    29941 cgtaatatac gccacctcgc gacagtgcgc taaag
//
LOCUS       DAKISA010000047        29794 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.047, whole genome shotgun sequence.
ACCESSION   DAKISA010000047 DAKISA010000000
VERSION     DAKISA010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29794
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.047"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(687..2351)
                     /gene="glnS"
                     /locus_tag="OVW45_002165"
     CDS             complement(687..2351)
                     /gene="glnS"
                     /locus_tag="OVW45_002165"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCV5526608.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2610..4556)
                     /gene="nagE"
                     /locus_tag="OVW45_002166"
     CDS             complement(2610..4556)
                     /gene="nagE"
                     /locus_tag="OVW45_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCV5526609.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVSDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGNVVAGQTPLYEIKK"
     gene            4889..5689
                     /gene="nagB"
                     /locus_tag="OVW45_002167"
     CDS             4889..5689
                     /gene="nagB"
                     /locus_tag="OVW45_002167"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCV5526610.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5749..6897
                     /gene="nagA"
                     /locus_tag="OVW45_002168"
     CDS             5749..6897
                     /gene="nagA"
                     /locus_tag="OVW45_002168"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCV5526611.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTSFTPDFKITKTIVNGNEVVTQ"
     gene            6906..8126
                     /gene="nagC"
                     /locus_tag="OVW45_002169"
                     /gene_synonym="nagR"
     CDS             6906..8126
                     /gene="nagC"
                     /locus_tag="OVW45_002169"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCV5526612.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            8174..8926
                     /gene="nagD"
                     /locus_tag="OVW45_002170"
     CDS             8174..8926
                     /gene="nagD"
                     /locus_tag="OVW45_002170"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HCV5526613.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            9323..10987
                     /gene="asnB"
                     /locus_tag="OVW45_002171"
     CDS             9323..10987
                     /gene="asnB"
                     /locus_tag="OVW45_002171"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCV5526614.1"
                     /translation="MCSIFGVFDIKTDAVEQRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            11367..11443
                     /locus_tag="OVW45_002172"
     tRNA            11367..11443
                     /locus_tag="OVW45_002172"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11401..11403,aa:Met,seq:cat)
     gene            11452..11536
                     /locus_tag="OVW45_002173"
     tRNA            11452..11536
                     /locus_tag="OVW45_002173"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11486..11488,aa:Leu,seq:tag)
     gene            11560..11634
                     /locus_tag="OVW45_002174"
     tRNA            11560..11634
                     /locus_tag="OVW45_002174"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11592..11594,aa:Gln,seq:ttg)
     gene            11669..11743
                     /locus_tag="OVW45_002175"
     tRNA            11669..11743
                     /locus_tag="OVW45_002175"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11701..11703,aa:Gln,seq:ttg)
     gene            11759..11835
                     /locus_tag="OVW45_002176"
     tRNA            11759..11835
                     /locus_tag="OVW45_002176"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11793..11795,aa:Met,seq:cat)
     gene            11884..11958
                     /locus_tag="OVW45_002177"
     tRNA            11884..11958
                     /locus_tag="OVW45_002177"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11916..11918,aa:Gln,seq:ctg)
     gene            11995..12069
                     /locus_tag="OVW45_002178"
     tRNA            11995..12069
                     /locus_tag="OVW45_002178"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12027..12029,aa:Gln,seq:ctg)
     gene            complement(12223..13398)
                     /gene="ubiF"
                     /locus_tag="OVW45_002179"
     CDS             complement(12223..13398)
                     /gene="ubiF"
                     /locus_tag="OVW45_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCV5526615.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQFQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            13544..14968
                     /gene="miaB"
                     /locus_tag="OVW45_002180"
     CDS             13544..14968
                     /gene="miaB"
                     /locus_tag="OVW45_002180"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCV5526616.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            15121..16161
                     /locus_tag="OVW45_002181"
     CDS             15121..16161
                     /locus_tag="OVW45_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCV5526617.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            16158..16625
                     /gene="ybeY"
                     /locus_tag="OVW45_002182"
     CDS             16158..16625
                     /gene="ybeY"
                     /locus_tag="OVW45_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCV5526618.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            16715..17593
                     /gene="corC"
                     /locus_tag="OVW45_002183"
     CDS             16715..17593
                     /gene="corC"
                     /locus_tag="OVW45_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCV5526619.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            17618..19156
                     /gene="lnt"
                     /locus_tag="OVW45_002184"
     CDS             17618..19156
                     /gene="lnt"
                     /locus_tag="OVW45_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCV5526620.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEVQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(19234..21111)
                     /locus_tag="OVW45_002185"
     CDS             complement(19234..21111)
                     /locus_tag="OVW45_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCV5526621.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            21566..22474
                     /gene="gltI"
                     /locus_tag="OVW45_002186"
     CDS             21566..22474
                     /gene="gltI"
                     /locus_tag="OVW45_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCV5526622.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHSDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTTAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            22644..23384
                     /gene="gltJ"
                     /locus_tag="OVW45_002187"
     CDS             22644..23384
                     /gene="gltJ"
                     /locus_tag="OVW45_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCV5526623.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            23384..24058
                     /gene="gltK"
                     /locus_tag="OVW45_002188"
     CDS             23384..24058
                     /gene="gltK"
                     /locus_tag="OVW45_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCV5526624.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            24058..24783
                     /gene="gltL"
                     /locus_tag="OVW45_002189"
     CDS             24058..24783
                     /gene="gltL"
                     /locus_tag="OVW45_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCV5526625.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            24901..25836
                     /gene="rihA"
                     /locus_tag="OVW45_002190"
     CDS             24901..25836
                     /gene="rihA"
                     /locus_tag="OVW45_002190"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCV5526626.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            25928..27598
                     /gene="hscC"
                     /locus_tag="OVW45_002191"
     CDS             25928..27598
                     /gene="hscC"
                     /locus_tag="OVW45_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HCV5526627.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(27658..29109)
                     /gene="djlC"
                     /locus_tag="OVW45_002192"
     CDS             complement(27658..29109)
                     /gene="djlC"
                     /locus_tag="OVW45_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCV5526628.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLCA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITQMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EDEKPQSITSIIKISLTVLMIGYALGKIAMLFS"
     gene            complement(29106..>29794)
                     /locus_tag="OVW45_002193"
     CDS             complement(29106..>29794)
                     /locus_tag="OVW45_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCV5526629.1"
                     /translation="LLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSSWGINNRD
                     DLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFVAQTAMCC
                     GEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYARAHHYYHSWMHYFSAYSL
                     GRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQPLTVQPEC
                     PVTLKDVSDL"
BASE COUNT         7360 a         7733 c         7503 g         7198 t
ORIGIN      
        1 cgatgaatcc ttcggcgcga gcctccggca ttgccataaa gcccgacatt gctgtaacac
       61 cggcgatagc cagcgccagc gtactacgtt tgccactaaa cgtacgcatg ggttaatcct
      121 ctttgacgta taaattgctg caccaaaggt gaacagcgaa aaaataaaaa ttaaaaaagt
      181 agcgttgaga aaagtctcgc tgactacagg aacagactat ttttcgcgac gcgaattatc
      241 aatctctttc tgtaaccaga atgtaagaac atgagcaacc gcacatattt tgatgatttt
      301 tgttacaagg cttaagtttg cggttgaaag gcttgcagaa gaataaaagc gtgaaattcc
      361 agcaagatga aagcggttac attttattta attatgtttt gttattttaa atttatttta
      421 tggataaatt gcgaagcgga attaattcgc gaagaaaagc agaaaaaacg ccgctgaacg
      481 cggcgttttg gattgtttgc atatttcttg ccggatgcgg cgtgaacgcc ttaaccggcc
      541 tacaaaagca cgcaaactca atatattgca gagatcatgt aggcctgata agcgtagtgc
      601 atcaggcaat ttagcgtttg aaactgtttg tctgttcctt gccggatgcg acgtaaacgc
      661 cccatccggc atagcgaaac ttaaaattac tcgcctactt tcgcccaggt atcacgcagc
      721 ccaacggtgc ggttaaatac cggtttttcc gccgtagaat ggcggctatc gaggcagaag
      781 taaccttcac gctcaaactg gaatgcttta cccgcaaccg catctttcag cgacggttca
      841 gcaaagccct gtttgatcac cagcgattcc gggttaatca ccgacaggaa atcatccgca
      901 gcaccagggt tcggcacgct gaacagacgg tcgtacagac ggatttcaac cggcagcgca
      961 tgtgccgcgc tcacccagtg aataacacct ttgactttac gaccatctgc cggatctttg
     1021 cttaaggtgt cggcgtcata agtacagaag atggtggtga tattgccttc ggcatctttc
     1081 tccacgcgtt ctgccttaat gacgtaagca ttacgcagac gcacttcttt acccagcacc
     1141 agacgtttgt actgcttgtt agcttcttcg cggaaatcgg cgcgatcgat ccaaatctca
     1201 ccgctaaacg gcacctggcg gctacccatt tccggtttgt tcggatggtt cggcatggta
     1261 accatttcgc cttcgccctg atagttttcg ataaccagtt tcaccggatc gataaccgcc
     1321 attgcacgcg gggcgttttc gttgagatct tcacggatgc aggattccag cgacgccatc
     1381 tcaatggtgt tgtcctgctt ggtcacgccg atgcgtttgc agaactcacg aatagaagca
     1441 gcagtgtaac cacgacgacg cagaccggaa atggtcggca tacgcgggtc atcccagcct
     1501 tcaacgtgct tgtcggtcac cagcaggttc agcttacgct tggacatcac agtgtattcc
     1561 agattcaggc gcgagaattc gtactggcgc gggtgaacag gaatggtgat gttgtccagc
     1621 acccagtcgt acaggcgacg gttgtcctgg aactcaagcg tacacagaga gtgcgtaata
     1681 ccttccaggg catcgctgat gcagtgggtg aagtcgtaca tcgggtagat gcaccacttg
     1741 ttgccagtct ggtggtgttc agcaaattta atgcggtaca gcaccggatc gcgcatcacg
     1801 ataaacggtg aagccatgtc gattttcgca cgcaggcagg ctttaccttc ttcaaaacca
     1861 ccggcacgca ttttttcgaa cagcgccagg ttctcttcaa cgctgcggtc gcggtacggg
     1921 ctgtttttac ccggttgcgt cagggtgccg cggtattcgc ggatctgttc cggcgtcagt
     1981 tcatcaacgt acgccaggcc tttattgatc agttcgatcg cataggcgtg gagctgatca
     2041 aaataatcgg aggagtaacg gacgttacca gaccagtgaa aacctaacca ctctacgtcg
     2101 tttttgatcg actcaacata ctcgatatct tcttttaccg ggttagtgtc gtcgaaacgc
     2161 aggttgcact ggcctttata gtcctgggcg atcccgaagt tcaggcagat agatttcgca
     2221 tggccaatat gcagatagcc attcggctcc ggcgggaaac gggtgtgtac tgtggtgtgc
     2281 ttaccactgg ccagatcttc atcgatgatc tgacggataa agttagtcgg gcgggcttct
     2341 gcctcactca tcgtggattc ctcaaagcgt aaacaacgta taacggcgta tgatcttata
     2401 agcgggacag gctgacaact gttagttagc gaaaatcgaa aatctcactg agattatggg
     2461 gatgattgat gaaaaaaaaa gcggcagaga agatctccac cgcttttgcc agtcccgcgt
     2521 cattgttgga tgcgcgtctt atccaaccag gactgagctt ttgtaggcct gataagacgc
     2581 gtcaagcgtc gcatcaggca taaagcagat tactttttga tttcatacag cggtgtttga
     2641 cccgccacaa cattgccctg agctttaatg atcaagccac tgaaatcgtc gatattgctg
     2701 caaaccaccg ggctaatcat cgaacgggcg ttagcgttca ggtaatccag atccatttcc
     2761 agaatcggtt gccctgcgct cacctgcgcg ccctcttcca ccagacgttt aaagccttta
     2821 ccttccagcg ctacggtgtc gatacccatg tggacgacaa tctccgcgcc tttttcggtt
     2881 tctaggcaga acgcgtggtt ggtgttgaag attttcacga ttgtccctgc ggctggtgat
     2941 acgacgattt tatctgtcgg tttcaccgcc acaccgtcac ccaccgcttt gctggcgaat
     3001 gcttcgtcag aaacctgatc cagtgccacg acatcaccgg taatcggcga taccagctcc
     3061 gcgatagata ccgcgtttgg tacagcctgc ggttttgcta caggagcggc agttgccgga
     3121 gttgcttcag ctgacgcagc tgctaccggg ccacgggcaa cgactttctt catcgcatcg
     3181 ccaatggatt ctgctttcgc gccaacaatc acctgaatgg tctgtttgtt cagttttact
     3241 acaccagaag cacccagacg tttacacatc gcatcgttga cgcgagctga gtcagccacg
     3301 gtaaggcgca gacgggtaat acaggcgtca atcgctttca ggttgtcagt accgccaacc
     3361 gcagcaatat agttggttgc cagttgagtc agaccttctt cagtattgct gttagcctct
     3421 tcagttacga tctcgtcttc tttatcttca cgacccggcg ttttcaggtt gaacatgcgg
     3481 ataaccaaac tgaacaccac gaagtagata gcgaagaaga caacacccat caccagcagc
     3541 atccagacgt tctggctggc ggccggcagg ttatacatca acgcgtagtc gatagccccc
     3601 gcagagaaag agaagccagc atggataccc agcagcgttg ccacaaacag gctgataccg
     3661 gtcagcagtg cgtgcaggag gtacagcagc ggagcaagga acatgaacag gaattccagc
     3721 ggctcagtca caccggtcag gaacgcagta acagcaacag aaagcagcat cccgccaacc
     3781 atcggacgac gctctttcgg tgctgcgaag tacatcgcca gcgccgcacc cggcagaccg
     3841 aacatcatga tcgggaagaa gccggacatg aacatccccg cggtgccgtc accagcgtag
     3901 aaacggttga tgtcgccgtg gaaaaccgta cccgccgcgt tggtgaattc accaatctgg
     3961 aaccaggcga tggtgttcag cacctgatgc agaccggttg ggatcagcag acggttgatg
     4021 aaaccaaaga taccggaacc cagcgcgcct gcagaaacga tccactcgcc gcctgcatgg
     4081 atagcgtgct gtactggcgg ccagacgtaa ccaaaaatgg ccgccagcac caggcagaag
     4141 aagccggtgg caatcggcac aaagcgtttg ccgccgaaga agctcaggaa gtccggcagt
     4201 ttaatatcgg accaacggtt ataggctgcg ccaccaacca gaccggtaat gatacccgcc
     4261 agtacaccca tgttaatttc tgggttgatg gtcaccatcg ctttggttaa cacaaagtaa
     4321 cctaccgcac ccgccagcgc cgccgcacct gcgctgtctt tcgaccagct ggatgccaca
     4381 ccgatggcga agattaatgc gaggttatca aaaatcgcac cgcccgcctg ggcaataaac
     4441 gcaacgttaa gtaaatctgg ctgaccgaat cgcagcaaca gtgccgccac cggcagcacc
     4501 gcgataggga gctgtaacgc cctaccgagt cgctggaaaa aacctaaaat attcatctta
     4561 ttccccctac gagaacccta tttggctcgt ttaaagccgt atttttattt tgctgcaaat
     4621 tgtactgccg atgttctgta atcagattgt tagatcatct gctacagagt gtgtgaaaat
     4681 ttaattcgta tcgcaaatta aacgcgtgcc ttttgtgagt tttgtcacca aatatcgtta
     4741 tcatcactcc cttttactgg ctaaagcaga aaacttattt tatcattcaa aaaatcaggt
     4801 cggattgacg cctgtctgcg caaatccagg ttacgcttaa agatgcctaa tccgccaacg
     4861 gcttacattt tacttattga ggtgaataat gagactgatc cccctaacta ccgctgaaca
     4921 ggtcggcaaa tgggctgctc gccatatcgt caatcgtatc aatgcgttca aaccgactgc
     4981 cgatcgtccg tttgttctgg gcctgccgac tggcggcacg ccgatgacca cctataaagc
     5041 gttagtcgaa atgcataaag caggccaggt cagctttaag cacgttgtca ccttcaacat
     5101 ggacgaatat gtcggtctgc cgaaagagca tccggaaagc tactacagct ttatgcaccg
     5161 taatttcttc gatcacgttg atattccagc agaaaacatc aaccttctca acggcaacgc
     5221 cccggatatc gacgccgaat gccgccagta tgaagaaaaa atccgttctt acggaaaaat
     5281 tcatctgttt atgggcggtg taggtaacga cggtcatatt gcatttaacg aaccggcgtc
     5341 ttctctggct tctcgtactc gtatcaaaac cctgactcat gacactcgcg tcgcgaactc
     5401 tcgtttcttt gataacgatg tcaatcaggt gccaaaatat gccctgactg tcggtgttgg
     5461 tacattgctg gatgccgaag aagtgatgat tctggtgctg ggtagccaga aagcactggc
     5521 actgcaggct gcagttgaag gttgcgtgaa ccacatgtgg accatcagct gtctgcaact
     5581 gcatccaaaa gcgatcatgg tgtgcgatga accttccacc atggagctga aagttaagac
     5641 tttaagatat ttcaatgaat tagaagcaga aaatatcaaa ggtctgtaat tgttatccct
     5701 gccctggctc cttgctcagg gcaatatttt ttaaaatcgg ggggcagaat gtatgcatta
     5761 acccagggcc ggatctttac cggccacgaa tttcttgatg accacgcggt tgttatcgct
     5821 gatggcctga ttaaaagcgt ctgtccggta gcggaactgc cgccagagat cgaacaacgt
     5881 tcactgaacg gggccattct ctcccccggt tttatcgatg tgcagttaaa cggctgcggc
     5941 ggcgtacagt ttaacgacac cgctgaagcg gtcagcgtgg aaacgctgga aatcatgcag
     6001 aaagccaatg agaaatcagg ctgtactaac tatctgccga cgcttatcac caccagcgat
     6061 gagctgatga aacagggcgt gcgcgttatg cgcgagtacc tggcaagaca tccgaatcag
     6121 gcgttaggtc tgcatctgga aggtccgtgg ctgaatctgg taaaaaaagg cacccataat
     6181 ccgaattttg tgcgtaagcc tgatgccgcg ctggtcgatt tcctgtgtga aaacgccgac
     6241 gtcattacca aagtgaccct ggcaccggaa atggttcctg cggaagtcat cagcaaactg
     6301 gcaaatgccg ggattgtggt ttctgccggt cactccaacg cgacgttgaa agaagcgaaa
     6361 gccggtttcc gcgcggggat tacctttgcc acccatctgt acaacgcgat gccgtatatt
     6421 accggtcgtg aaccgggcct ggcgggcgcg atcctcgacg aagctgacat ttattgcggt
     6481 attatcgctg atggcctgca tgttgattac gccaacattc gtaacgctaa acgccttaaa
     6541 ggcgacaaac tgtgtctggt taccgacgcc accgcgccag caggtgccaa cattgaacag
     6601 ttcatttttg cgggtaaaac aatatactac cgtaacggac tttgtgtgga tgagaacggt
     6661 acgttaagcg gttcatcctt aaccatgatt gaaggcgtgc gtaatctggt cgaacattgc
     6721 ggtatcgcac tggatgaagt gctgcgtatg gcgacgctct atccggcgcg tgcgattggc
     6781 gttgagaaac gtctcggcac actcgccgca ggtaaagtag ccaacctgac ctcattcaca
     6841 cctgatttta aaatcaccaa gaccatcgtt aacggtaacg aggtcgtaac tcaataagag
     6901 aaagtatgac accaggcgga caagctcaga taggtaatgt tgatctcgta aaacagctta
     6961 acagcgcggc ggtttatcgc ctgattgacc agtacgggcc aatctcgcgg attcagattg
     7021 ccgagcaaag ccagcttgcc cccgccagcg taaccaaaat tacgcgtcag cttatcgaac
     7081 gcgggctgat caaagaagtt gatcagcagg cctccaccgg gggccgccgc gctatctcca
     7141 tcgtcaccga aacccgcaat ttccacgcaa tcggcgtacg gcttggtcgt cacgacgcca
     7201 ccatcaccct gtttgatctc agcagcaaag tgctggcaga agaacattac ccgctgccgg
     7261 aacgtaccca gcaaacgctg gaacatgccc tgttgaatgc cattgctcag tttattgata
     7321 gctaccagcg caaactgcgc gagctgatcg cgatttcggt gatcctgcca gggcttgttg
     7381 acccggacag cggcaaaatt cattacatgc cgcatattca ggtagaaaac tgggggctgg
     7441 tagaagctct ggaagaacgt tttaaagtga cctgtttcgt tggtcacgat atccgtagtc
     7501 tggcgctggc ggagcactac ttcggtgcaa gtcaggattg cgaagactcc attctggtgc
     7561 gtgtccatcg cggaaccggg gccgggatta tctctaacgg gcgcattttt attggccgca
     7621 acggtaacgt cggtgaaatt ggccatattc aggtcgaacc gctgggtgaa cgctgccact
     7681 gcggcaactt tggctgcctg gaaactatcg ccgccaacgc tgccattgaa caacgggtgt
     7741 tgaatctgtt aaagcagggc taccagagcc gcgtgccgct ggacgactgc accatcaaaa
     7801 ctatctgcaa agccgcgaac aaaggcgata gtctggcgtc ggaagtaatt gagtatgtcg
     7861 gtcgtcatct gggtaaaacc atcgccattg ctatcaactt atttaatccg caaaaaattg
     7921 ttattgccgg tgaaatcacc gaagccgata aagtgctgct ccctgctatt gaaagctgca
     7981 ttaataccca ggcgctgaag gcgtttcgta ctaatctgcc ggtggtacgt tctgagctgg
     8041 atcaccgctc ggcaatcggc gcttttgcgc tggtaaaacg cgccatgctc aacggtattt
     8101 tgctccagca tttgctggaa aattaatgtg cttttatagt ggcgcttatt gttgtcaata
     8161 ttctgggtag tccatgacca ttaaaaatgt aatttgcgat atcgacggcg tgctgatgca
     8221 cgataacgtc gccgtaccgg gtgcagcgga atttttgcac gggattatgg ataaaggcct
     8281 gccgctggtg ttgctgacca actatccttc gcagaccggg caagatctgg cgaaccgctt
     8341 tgccactgcc ggtgtcgatg tgccggatag cgtgttttat acctctgcga tggcgaccgc
     8401 cgatttcctg cgtcgccagg aaggcaagaa agcatatgtg gtgggcgaag gcgcactgat
     8461 tcatgaactg tacaaagccg gtttcactat taccgatgtg aaccctgatt tcgtgattgt
     8521 tggcgaaacg cgttcctaca actgggacat gatgcataaa gcggcctatt tcgtcgctaa
     8581 cggtgcacgc tttatcgcca ccaacccgga cacccacggg cgcggttttt atcccgcttg
     8641 tggcgcgttg tgtgcaggga ttgagaaaat ctccgggcgc aaaccgttct atgttggtaa
     8701 gcccagcccg tggatcatcc gcgcagcatt aaacaaaatg caggcgcatt cggaagaaac
     8761 ggtgattgtc ggcgataact tgcgcaccga cattctggcg ggcttccagg caggtctgga
     8821 aacgattctg gtgctttctg gtgtttcatc gctcgacgat atcgacagta tgccgttccg
     8881 cccaagctgg atttacccgt cagtcgctga aatcgacgtt atctgaatac cacccacgtc
     8941 tgcggacgtg gttggctctg atgacaaacg ccaaactgcc tgatgcgcta cgcttatcag
     9001 gcctacgcag ctcctgcaat atattgaatt ttcatgcttt tgtaggccgg ataaggcgtt
     9061 cacgccgcat ccggcatgaa caaatcgcac tttatcagca atttggcccc cgtaaaatat
     9121 aaccattcaa taaaacccgc taaaaattat cgattcatca ataaatacat cctcacacca
     9181 gccccttttt caccaatcga caatcaccat tacgtttttt attttttcac cgccaaatcg
     9241 cttgcgcaca gtgctgcttt gcggcatttt tttaaacaag caaacacaac aagcaacaaa
     9301 taccaggtta acggagaagg ttatgtgttc aatttttggc gtattcgata tcaaaacaga
     9361 cgcagttgag cagcgtaaga aagcactcga gctgtcacgc ctgatgcgtc atcgtggccc
     9421 ggactggtcc ggtatttatg ccagcgataa cgccattctc gctcacgaac gtctgtcaat
     9481 tgttgacgtt aacgcggggg cgcaacctct ctacaaccaa caaaaaaccc acgtactggc
     9541 ggtaaacggt gaaatctaca accaccaggc attgcgcgct gaatatggcg atcgttatca
     9601 gttccagaca gggtctgact gtgaagtgat cctcgcgctg tatcaggaaa aagggccgga
     9661 atttctcgac gacttgcagg gcatgtttgc ctttgccctg tacgacagcg aaaaagatgc
     9721 ctacctgatt ggtcgcgacc atctggggat catcccactg tatatggggt atgacgaaca
     9781 cggtcagctg tatgtggcct cagaaatgaa atcgctggtg ccagtttgcc gcacgattaa
     9841 agagttcccg gcggggagct atttgtggag ccaggacggc gaaatccgtt cttattatca
     9901 tcgcgactgg ttcgactttg atgcagtgaa agataacgtg accgacaaaa acgagctgcg
     9961 tcaggctctg gaagattccg ttaaaagcca tctgatgtct gatgtgcctt acggtgtgct
    10021 gctttctggt ggtctggatt cctcaattat ttccgctatc accaagaaat acgcagcccg
    10081 tcgcgtggaa gatcaggaac gctctgaagc ctggtggccc cagttacact cctttgctgt
    10141 aggtctgccg ggttcaccgg atcttaaagc agcccaggaa gtggcaaacc atctgggcac
    10201 ggtgcatcac gaaattcact tcactgtaca ggaaggtctg gatgccatcc gcgacgtgat
    10261 ttaccacatc gaaacttatg atgtgaccac tattcgcgct tcaacaccga tgtatttaat
    10321 gtcgcgtaag atcaaggcga tgggcattaa aatggtgctg tccggtgaag gttctgatga
    10381 agtgtttggc ggttatcttt acttccataa agcgcccaac gctaaagaac tgcatgaaga
    10441 gacggtgcgt aaactgctgg ccctgcatat gtatgactgc gcgcgcgcca acaaagcgat
    10501 gtcagcctgg ggcgtggaag cacgcgttcc gttcctcgac aaaaaattcc ttgatgtggc
    10561 gatgcgcatt aacccgcagg ataaaatgtg cggtaacggc aaaatggaaa aacacatcct
    10621 gcgtgaatgt tttgagtcat acctgcccgc aagcgtggcc tggcggcaga aagagcagtt
    10681 ctccgatggc gtcggttaca gttggatcga caccctgaaa gaagtggcgg cgcagcaggt
    10741 ttctgatcag caactggaaa ctgcccgctt ccgcttcccg tacaacacgc caacctcaaa
    10801 agaagcgtat ctgtaccggg agatctttga agaactgttc ccgcttccga gcgccgctga
    10861 gtgcgtgccg ggcggcccgt ccgtcgcgtg ttcttccgct aaagcgattg agtgggatga
    10921 ggcgttcaag aaaatggacg atccgtctgg tcgtgcggtt ggcgttcacc agtcggcgta
    10981 taagtaagaa atgcgaaaca gccccggtga aatccggggc tgtttcagtt attgaggtgc
    11041 ttgttgattc tgatatgcag taaggaaatg ttccgggttt gccacatcac tgtttgacaa
    11101 ctcatcactg taggccagta tttcatagat taatttccca ccaccattgt tgaattccga
    11161 atttcatcct gtatgacaaa agaatggctg tagggtgggg aaaaaacaca aagtaaacaa
    11221 taattgacga atatagcgcc acgctgttcg caacctaacc aaacagtcac tttcgagcaa
    11281 ttttccttga aaaagaggtt gacgctgcaa ggctctatac gcataatgcg ccccgcaacg
    11341 ccgataaggt atcgcgaaaa aaagatggct acgtagctca gttggttaga gcacatcact
    11401 cataatgatg gggtcacagg ttcgaatccc gtcgtagcca ccatcttttt tgcgggagtg
    11461 gcgaaattgg tagacgcacc agatttaggt tctggcgccg caaggtgtgc gagttcaagt
    11521 ctcgcctccc gcaccattca ccagaaagcg ttgtacggat ggggtatcgc caagcggtaa
    11581 ggcaccggtt tttgataccg gcattccctg gttcgaatcc aggtacccca gccatcttct
    11641 tcgagtaagc ggttcaccgc ccggttattg gggtatcgcc aagcggtaag gcaccggttt
    11701 ttgataccgg cattccctgg ttcgaatcca ggtaccccag ccatcgaaga aacaatctgg
    11761 ctacgtagct cagttggtta gagcacatca ctcataatga tggggtcaca ggttcgaatc
    11821 ccgtcgtagc caccaaattc tgaatgtgtc gaatacgttc ggcaaattca aaaaccaatt
    11881 tgttggggta tcgccaagcg gtaaggcacc ggattctgat tccggcattc cgaggttcga
    11941 atcctcgtac cccagccaat ttattcaaga cgcttacatc gtaagtgcac cagttggggt
    12001 atcgccaagc ggtaaggcac cggattctga ttccggcatt ccgaggttcg aatcctcgta
    12061 ccccagccac attaaaaaag ctcgcttcgg cgagcttttt gcttttctgc gttcattcaa
    12121 tgtcgaatgc gatgttgaca cgtcttatcc ttcaatgtcg gatgcgacgc tgccgcgtct
    12181 tatccgacct acggttggca cgcatccggc aatgttgtaa ggctacaacc ctaacgcata
    12241 tttcagcgcc tgacgtttca acacgccagc acgctctgcc gccattaacc cgagattacg
    12301 cacaaaacgc agcggcggca gattattact gaatccggca taaaacagat ccataccact
    12361 ttgcataatg aagttatccg ccatgcgccg catctggtaa cgcttgagga caggataact
    12421 ggcccacgct tcaccgtagc tgcgggcatt caccagaaca tcaatcaggg catcgacatc
    12481 acgataacca agattcaccc cctgccccgc cagcggatgg atggtatgcg cggcatcgcc
    12541 caccagcgca agccccggct gcacatactg caacgcatgt cggcgcgtca gcggaaacgc
    12601 accagcggca agcggtgtaa cgtaacccag acgcgacggg aaatgcttcg cgatttccgc
    12661 ctggaattgc gccatattca tattctgcaa ctggcgaata cgcgccggag agtcatacca
    12721 caccagcgat gcccagttat caaacaacgg cagaaacgca cgcggtccgt ccggggtaaa
    12781 ttgctgccag gtgctgtcgc cgggatcgtt ctcacactgt acgctaatca acatgcacga
    12841 ctgcgcatac tgccaggcat gaacgccaat tcccgccatc tgccgcacct gagaatttgc
    12901 gccgtcggca ccaatcacca gtttcgcgcg gatcgtttca ccgcccttca gttccagctc
    12961 ctgaagatca ttatctcgat gtaatgcaat cagcgagcct ggcacacgta acgttacttt
    13021 cggatgcgct tccagcgcct gccacagcgc ctgttgcagg acggtgtttt ccaccatata
    13081 gccaagcaac ggtagcttaa gttcagcggc gtcaaacacc acatgcgccg tttcccactc
    13141 ccacgtttcc agtctgcggt aaggatggca acgcatagcc tgtactgcat cccagacccc
    13201 taacccttta agcaatgata ccgatgcggc gctgatcgcc gagatccgca cgtccggttg
    13261 gctatcagcg acaaacggtg caggttctgc gtgctcgatc accgttaccg aaaatccgtg
    13321 ctgtgccagc cccagcgcca gcgcgccgcc gaccattcct ccgccgacaa tggcaatttc
    13381 cgttggttga tttgtcatgg tcaatcatcc tgttagcaat atgctttaag tttacaggat
    13441 ttttaccccc aagggctgat aacgctcata ctggtcacaa cggcagcaaa gcattacact
    13501 atacgccctg cattcctggc tactatttcg caagagcaag tcgatgacca aaaaactcca
    13561 tattaaaacc tggggctgtc agatgaacga gtacgattca tcgaagatgg ccgatctgct
    13621 ggatgccacc cacggctatc aactgaccga cgtggcggaa gaagcggatg tgctgctgct
    13681 gaacacctgc tcaatccgcg agaaggctca ggaaaaagtc ttccatcagt tgggtcgctg
    13741 gaaactgtta aaagagaaga atccagacct gattatcggc gtcggtggct gcgtggcatc
    13801 gcaagaaggc gagcacattc gccagcgcgc ccactatgtc gatattattt ttgggccgca
    13861 aacgctgcac cgtctgccgg agatgatcaa ctccgtgcgc ggcgaccgca gcccggttgt
    13921 agatatcagc ttcccggaaa tcgagaagtt tgaccgtcta ccggaaccgc gcgccgaagg
    13981 gccgaccgcg tttgtctcca tcatggaagg ctgcaataaa tattgcacct actgcgtggt
    14041 gccttacacc cgtggtgaag aggtaagccg tccgtctgac gatattctgt ttgagattgc
    14101 ccagcttgcg gctcagggcg tgcgtgaagt caacctgctc ggtcagaacg tgaacgcctg
    14161 gcgtggtgaa aactacgacg gctccaccgg atcgtttgcc gatctgctgc gtctggttgc
    14221 tgcgatcgac gggatcgatc gtattcgctt taccaccagc catccgatcg aattcaccga
    14281 cgatatcatc gaagtgtatc gcgacacgcc ggagctggtg agcttcctgc atctgccggt
    14341 acaaagcggt tccgatcgca ttctgaacct gatgggccgt actcacacgg cgctggagta
    14401 caaagcgatc atccgtaaac tgcgtgcggc gcgtccggat attcagatca gttctgactt
    14461 cattgttggc ttccctggcg aaaccaccga agacttcgag aaaacgatga agctgattgc
    14521 cgacgtcaat ttcgacatga gctacagctt tatcttctct gcacgtccgg gtacaccagc
    14581 cgccgatatg gttgatgatg ttccggaaga agagaagaag caacgtctgt atattctgca
    14641 agaacgtatc aatcagcaag cgatggcatg gagccgccgg atgctcggca ccacccagcg
    14701 tattctggta gaagggactt cgcgtaagag catcatggag ctttccgggc gtaccgaaaa
    14761 taaccgcgtg gtcaacttcg agggcacacc ggatatgatc ggtaaattcg tcgatgtaga
    14821 aattaccgac gtctacccga actctctgcg cggtaaagtg gtgcgtactg aagatgaaat
    14881 gggtctgcgc gtggcagaaa caccggaatc ggtgattgcc cgtacccgca aagaaaacga
    14941 ccttggcgtg ggttattatc agccgtaatt ctcaggcccg ccgttccggt gggccttgtt
    15001 ttttctctct ttatccccat ctttatcgca atgcttgcgc ctttttcctg tggcgtgaat
    15061 aatttcctta taggccggtg aatgttcagc ttccggcggc atacagataa gaggaacggt
    15121 ttgaacatag acactcgcga aatcaccctg gagccagcag acaacgcgcg tctgttgagc
    15181 ctgtgcggcc cgtttgatga caacatcaag cagctcgaac gccgtctcgg catcgagatc
    15241 aatcgccgcg acaaccactt taaactgacc ggccgtccga tttgcgtcac cgctgcggca
    15301 gacattctgc gtagcctgta tgtcgataca gccccgatgc gcggtcagat tcaggatatc
    15361 gaaccggaac agatccacct tgcgattaaa gaagcgcgag tactggagca aagtgcggag
    15421 agcgtgccgg agtacggcaa agcggtcaat atcaaaacca aacgcggcgt aattaagccg
    15481 cgcacgccaa accaggcgca gtacatcgcc aatattctcg accatgacat taccttcggc
    15541 gttggcccgg cgggtacggg taaaacctac ctggcagtgg ctgcggcagt tgatgccctg
    15601 gagcgtcagg agattcgccg tattctgctg actcgtccgg cggtcgaagc cggtgagaaa
    15661 ctgggcttcc tgcctggcga tttaagccag aaagtagacc cgtatctgcg cccactgtac
    15721 gacgcgctgt ttgaaatgct gggctttgag aaagttgaga aactgattga gcgcaacgtt
    15781 attgaagtcg cgccgctggc ctatatgcgt ggtcgtacgc tgaacgacgc gtttatcatt
    15841 ctcgatgaga gccagaacac caccatcgaa cagatgaaga tgttcctgac ccgtatcggt
    15901 tttaactcaa aagcggttat caccggcgac gtcacacaga tcgacctgcc gcgtaatact
    15961 aaatcaggct tacgtcacgc catcgaagtg ctggccgatg tcgaagagat cagctttaac
    16021 ttcttccaca gcgaagacgt ggttcgtcac cctgtggtgg cgcgtatcgt taacgcctat
    16081 gaagcctggg aagaagccga acaaaaacga aaagcggcgc tggcggcaga acgcaagcgc
    16141 gaagaacagg aacaaaaatg agtcaggtga tcctcgattt acaactggca tgtgaagata
    16201 attccgggtt accggaagag agccagtttc aaacatggct gaatgcggtg atcccgcagt
    16261 ttcaggaaga atcggaagtg actattcgcg tggtcgatac cgccgaaagc cacagtctga
    16321 atctgaccta tcgcggtaag gataagccga ccaacgtgct ctccttcccg tttgaagtgc
    16381 cgcctggcat ggaaatgtcg ctactgggcg atctggttat ctgccgtcag gtggttgaga
    16441 aggaagctca ggagcaaggc aaaccactgg aggcgcactg ggcgcatatg gtggtgcacg
    16501 gcagtctgca tttgttaggt tacgatcaca tcgaagatga cgaagcagaa gaaatggaag
    16561 ccctcgaaac agagattatg cttgctctgg gctatgagga tccgtacatt gccgagaaag
    16621 aataatttgc caggcatttg actggcagac gtccccgccg ttggtgatta acgacggcgt
    16681 tttgattaac taacatgaga acccatacga cgccatgagc gacgacaatt cacacagtag
    16741 tgacacgata agcaacaaga agggattttt ctccctgtta ctcagccaac ttttccacgg
    16801 tgaaccgaaa aaccgtgacg aactgctggc gctgatccgt gattccgggc agaacgacct
    16861 tatcgacgaa gatacgcgcg atatgctcga aggggtgatg gacatcgcag accaacgcgt
    16921 tcgcgacatc atgatccccc gctcccagat gattaccctg aaacgcaacc agacgctgga
    16981 cgaatgcctt gatgtcatca tcgagtccgc ccactcacgt ttcccggtga ttagcgaaga
    17041 caaagatcac attgaaggga ttctgatggc gaaagacttg ctgccgttta tgcgcagcga
    17101 tgctgaagcc ttcagcatgg acaaagtgtt acgtcaggcg gttgtcgttc ctgaaagtaa
    17161 gcgcgtagac cggatgctga aagagtttcg ctctcagcgt taccacatgg cgatcgtcat
    17221 tgacgaattc ggtggggttt ccggtctggt gaccattgaa gacatcctgg aactgattgt
    17281 tggtgagatt gaagacgagt atgacgaaga agatgatatc gacttccgtc agctgagtcg
    17341 tcatacctgg accgtgcgtg cactggcttc cattgaagac ttcaacgaag cgttcggcac
    17401 ccactttagc gatgaagagg ttgacactat cggtggtctg gtgatgcagg catttgggca
    17461 tcttccggcg cgtggcgaaa ctatcgacat cgacggttac cagttcaaag tggcgatggc
    17521 cgacagtcgg cgtattattc aggttcatgt caaaatcccg gatgactcac cccagccgaa
    17581 gctggatgaa taaaaccgaa actggataga taactacatg gcttttgcct cattaattga
    17641 acgccagcgc attcgcctgc tgctggcgtt attattcggt gcctgcggaa cgctggcctt
    17701 ctctccttac gacgtctggc ctgcggcgat tatttcgttg atggggcttc aggcgttgac
    17761 ctttaaccgc cgtccactcc agtctgccgc tattggcttt tgctggggat ttggcctctt
    17821 tggcagcggt attaactggg tctatgtcag catcgcgacc tttggcggaa tgcctggccc
    17881 ggttaacatc ttcctggtgg tgctgctggc ggcgtatttg tcgctgtata ccggactgtt
    17941 tgctggcgtg ctgtcgcgtc tgtggccgaa aaccacctgg ctgcgcgtgg cgattgccgc
    18001 ccctgctctc tggcaagtga ccgagtttct gcgcggttgg gtactgacag gcttcccgtg
    18061 gttacagttc ggctatagcc agattgatgg cccgttaaaa gggctggcac cgataatggg
    18121 cgtggaagcc attaacttcc tgctgatgat ggttagtggc ctgctggcac tggcgttggt
    18181 caaacgcaac tggcgtccgc tggtggtggc cgtcgtgctg tttgccctac ccttcccgct
    18241 gcgttacatc caatggttta ccccgcaacc ggagaaaacc attcaggttt cgatggttca
    18301 gggcgatatt ccgcaatcac tgaaatggga tggagatcag cttcttaata cgctgaagat
    18361 ttactacaac gcaacggctc cgctgatggg caaatcatcg ttgattatct ggccggagtc
    18421 ggcgataacc gatctggaaa ttaatcagca accgttcctc aaagcactgg acggtgagtt
    18481 gcgcgataaa ggtagctcgc tggtaaccgg gattgtcgac gcgcgtctca ataagcagaa
    18541 ccgctacgat acctacaaca ccatcatcac actgggtaaa ggtgcaccat acagctacga
    18601 atcagccgat cgctataaca aaaaccatct agtgccgttt ggcgagtttg tcccgctgga
    18661 gtcgattctg cgtccgttag caccgttctt tgatctgccg atgtcgtcgt tcagccgtgg
    18721 gccatatatc cagccgccgc tgtcggtaaa tggtattgag cttactgcgg ctatttgcta
    18781 cgagatcatt ctcggcgagc aagtgcgcga taacttccgc ccggataccg actatctgct
    18841 gactatctcc aacgatgcgt ggtttggtaa gtctattggt ccatggcagc acttccagat
    18901 ggcgcgaatg cgtgcgctgg agctggcgcg cccactgttg cgcagcacca acaacggcat
    18961 tacggcggtg attggcccgc agggggaggt tcaggcgatg atcccgcagt tcacccgcga
    19021 ggtgttgacc actaatgtga caccgaccac cggactcaca ccgtatgcac gtaccggcaa
    19081 ctggccactg tgggtgctga cggcgctgtt tggttttgcc gctgtgttga tgagtctgcg
    19141 agcacgtaaa cgttgatgtg cctgatgtat cctgcatgag caaaacgtac tttctcattg
    19201 agctttgggg catggttatc atgccccttt ttcttacggt tcagggatac aagccacatt
    19261 gccatattgg ggatgacgaa taattgcctg cggatcaagc gagcgcgtcc tgtccatttg
    19321 gatcaacccg ttcttcgcca tgacaaatat ttttctacca tccgctgaaa aacgaaggtg
    19381 attaacgtag ctggcaggat aaacgccaat aatctgcccg gcattaatat tgagcaggat
    19441 gacataacct tgcaggccat atgacaccaa cgctaaatat tgaccgtccg ggctaatacc
    19501 ctcaacggaa taaacgctcc tgctacacca ttgcgtcata atgctggctt gttgatcccc
    19561 tacgcttata cgtgaaatcc cctgactatc ggcaaaaata acctcgccgt tttcaggtcg
    19621 aaaacgcatc gccgatatcg ttccacgcgc atcacctgcg ggaaagtctt ctttctgatg
    19681 cgtgatggta ttgatccgca cgatgttacc tctgcctgct cccggaacca gtaatgttct
    19741 ttcatccggg gaaagtgcca taacccaggc gcctggttta ccgctcgtcc ccgtcccgtc
    19801 gtatttctcc agcgggatat ccgccacatg ctgataagtc accagatcaa tcgctgacac
    19861 actattactg gtgcgattca tcacccaggc ttttgtgcca tcacgcgaga ggataaggct
    19921 gtcaggatgt gcaccggtgg taatcgactg aataatctcc cctgtcgcca cgttaatgac
    19981 cgataacgca ttcgcttcaa atgaagtcgc ataaatcaac ttttcatccg ggcttaatgc
    20041 cagcgatctg acgccctgat ctttacagcc accacaatgt ttgactgact tttctttcgc
    20101 tatgggcgct gtaaaggtat gcaggatttt cttttgtccg aggtcgaaaa caacaatgct
    20161 gttcgtgttt tcaattgcgg catacaacat attaccatcc cgactgatcg ccatatcatg
    20221 gatatcggca atctcagcga gcaaatcacc actggcctgt tcacgactta ccggagcgga
    20281 taatgtttcc ctgagggcgt tgcgttcctc cgctaactgc tgcttttttt gccgtcgctc
    20341 ttgttcaatc tcctgtggat aaaacgcttc acgcaggctt tgcctgacct gtaaaactga
    20401 ctcatccatc tgctgctgcg caagccagat agccccggtc agaagaagac tgcccgcaac
    20461 aataatcccg ccttccgtaa cgagtcggtt ttgcccagat aaacgcgcgc tcagccaacc
    20521 caacgcgcca cccgcaatca ggccgccaat gtggcaagcg ttatctattc ctgtttgccg
    20581 ggcaccgtac aacagtgtca gcgccaccat acccaacaga ggaaatactc gccgctggtt
    20641 tttatggtat gtgcccgtac ctgaagcaaa ctgtgttgct atcgctgcac cagctattcc
    20701 cagaatcgcc ccggatgccc caacgctgat gactatctgc tcactttcgc gtaacgcata
    20761 acaggcgctg atgagcccac caaagacgcc ggagaataac caggtaatca ggagtcgcca
    20821 tttccccaga atctgttccg ccacgcctcc cacgaccagg aatgcaaaca tattcatcag
    20881 cagatgagaa acactactgt gcagaaagat actgctcaat aagcgccagg gttcacccga
    20941 caaagtaaga ggcgcaacgt ttgcgccgag atggataagt gcggattgat cgacctcttc
    21001 ataactcatc cattgtgcga gataacaaaa tatcccaata cagagtgcaa aaaggataag
    21061 ggttattgcg ggtaattgaa catttaacgg cttaaccgaa gatgcagaca taaggtgttt
    21121 cttccttgaa aagacaggta aaaagcattt ttcccgtgtg cagttatcat cccggcaaac
    21181 gtcccatgcc gaaaagataa ttctgtccga aatatgactt tttcagccta aaattacaat
    21241 cgtaattaat tcgcccctaa gtattttttg gcatgtaagt tgctttattt actgtgtaag
    21301 cgatgaaatc agccgcgacg caaccaggtt gcaccaccac aaatcagcgg tagcaccaaa
    21361 aaggtgcggt ttaagaattt tgcttcggaa tggtgcggcg catctcgcaa aaacaaacaa
    21421 taaaacagca atatctttac atttagccaa actaaatgtt acctaacagg cacaacactg
    21481 cacaataaag ttgcagacga taacaacaca aacactcaca acgggtatcc atgcgttctt
    21541 aacgcagaag ataaaggagt tggatatgca attacgtaaa cctgccacag caatcctcgc
    21601 cctggcgctt tccgcaggac tggcacaggc agatgacgcc gccccggcag caggcagtac
    21661 gctggacaaa atcgccaaaa acggtgtgat tgtcgtcggt caccgtgaat cttcagtacc
    21721 tttctcttat tacgacaatc agcaaaaagt ggtgggttac tcgcaggatt actccaacgc
    21781 cattgttgaa gcagtgaaaa agaaactcaa caaaccggac ttgcaggtaa aactgattcc
    21841 gattacctca caaaaccgta ttccactgct gcaaaacggc actttcgatt ttgaatgtgg
    21901 ttctaccacc aacaacgtcg aacgccaaaa acaggcggct ttctctgata ctattttcgt
    21961 ggtcggtacg cgcctgctga ccaaaaaggg cggcgatatc aaagattttg ccgacctgaa
    22021 agacaaagcc gtagtcgtca cttccggcac tacctctgaa gttttgctca acaaactgaa
    22081 tgaagagcaa aaaatgaata tgcgcatcat cagcgccaaa gatcacagtg actctttccg
    22141 caccctggaa agcggtcgtg ccgttgcctt tatgatggat gatgccctgc tggccggtga
    22201 acgtgcgaaa gcgaagaaac cagacaactg ggaaatcgtc ggcaagccgc agtctcagga
    22261 agcctacggt tgcatgttgc gtaaagacga tccgcagttc aaaaagctga tggatgacac
    22321 caccgcgcag gtgcagacct ccggtgaagc ggaaaaatgg tttgataaat ggttcaaaaa
    22381 tcccattccg ccgaaaaacc tgaacatgaa tttcgaactg tcagacgaaa tgaaagcact
    22441 gttcaaagaa ccgaatgaca aggcactgaa ctaattacaa gaaccagggg cggaaattcc
    22501 agccctctcg attgttacgt agcacggaca gactatacgc ctgatggtcg ttccccatcg
    22561 ggcctgaaaa ccgcaatacg ctgggtaaca atcttcgagg gtagcagtta acgctgctac
    22621 cctctttttt ctggagtaga tttatgtcta tagactggaa ctggggtatt tttttacaac
    22681 aagccccgtt cggcaacacc acctatcttg gttggatctg gagtggtttt caggtgacga
    22741 tcgctttgtc gatctgcgcc tggattatcg ctttcctcgt cggttcattt ttcggcattt
    22801 tacgtaccgt tccaaaccgt tttctctccg gtctgggtac gttgtatgtc gaactgttcc
    22861 gcaacgtgcc gttgattgtg caattcttta cctggtatct ggtgatcccg gagctgttgc
    22921 cggagaaaat cggcatgtgg tttaaggccg agctggatcc caatattcag tttttccttt
    22981 catccatgct ctgcctgggg ctgtttaccg ccgcccgtgt ttgcgaacag gtacgcgccg
    23041 ccattcagtc gttgccgcgt gggcaaaaaa atgccgcgct ggcgatgggc ctgacgctac
    23101 cgcaggctta tcgatacgta ctgctgccta atgcttatcg cgttatcgtc ccgccgatga
    23161 cttccgagat gatgaacctg gtgaaaaact ccgccatcgc ctcgactatt ggtctggtgg
    23221 atatggcggc gcaggcgggt aaattgcttg attactcagc ccacgcctgg gagtcgttta
    23281 ccgctattac gctggcgtat gtgttgatta acgcggttat catgctggta atgacgctgg
    23341 ttgaacgtaa agttcgcctg cctggcaaca tggggggcaa ataatgtacg agtttgactg
    23401 gagttccatt gtcccttccc tgccatatct gctcgacggg ctggtgatca cgctgaaaat
    23461 caccgtcacg gcagtcgtga ttggtatttt gtggggcacc atgctggcgg tgatgcgctt
    23521 gtccagcttt gcgcccgtag cgtggtttgc caaagcctac gttaacgtat tccgctcgat
    23581 tcctttagtt atggttttgc tgtggtttta cctgatcgtg ccaggttttc tgcaaaacgt
    23641 gctgggatta tcaccaaaaa atgatatccg tttaatttcg gcgatggtgg cgttttcgat
    23701 gtttgaagcg gcctactatt cagagattat ccgtgctggt attcaaagta tttctcgtgg
    23761 tcagtcgagc gccgcgctgg cgttgggaat gactcactgg cagtcgatga aactgattat
    23821 tctgccgcag gcgttccgcg cgatggtgcc gcttctgctc actcagggca tcgtactgtt
    23881 ccaggatacc tcactggtgt atgtgttatc tctggccgat ttcttccgta ccgcctcaac
    23941 cattggtgag cgtgatggta cgcaggttga gatgatcctg tttgccggat ttgtttattt
    24001 cgttattagt cttagcgcgt cgttgttggt cagctacttg aaaagaagga cagcataatg
    24061 attaccctga aaaatgtttc aaaatggtat ggtcactttc aggtgctgac cgactgctca
    24121 accgaagtga aaaaaggcga agtggtggtg gtttgcggcc cgtctggttc cggcaaatca
    24181 acgctgatta aaaccgtcaa cggcctcgaa ccagtgcagc aaggtgaaat caccgtcgat
    24241 ggtatcgtgg ttaatgacaa gaaaaccgat ctggcaaagc tgcgttcccg cgtcgggatg
    24301 gtattccagc atttcgagct gttccctcat ctgtcgatta tcgaaaacct gaccctggcg
    24361 caggtgaaag tgcttaaacg cgataaagcg cccgctcgtg aaaaagcatt gaaattgctg
    24421 gagcgcgtgg gactttccgc acatgccaat aaatttccgg cgcagctttc cggcggtcag
    24481 cagcagcgtg tggcaatcgc tcgcgcattg tgtatggatc ctattgcgat gctgttcgac
    24541 gaaccgacat cggcgctgga tccggagatg atcaacgaag tgctggacgt gatggtggaa
    24601 ctggcgaacg aaggaatgac catgatggtg gtgacccacg aaatgggctt tgcccgcaaa
    24661 gtggcgaatc gggtgatctt tatggacgag ggtaaaattg tcgaagactc gccgaaagac
    24721 gctttcttcg atgatccgaa atcggaccgc gcgaaagact tcctcgcgaa aatcctgcat
    24781 taatcactct ggcgcgcata cccaagtgcg cgccatctca cgctttatcg tttcgcccct
    24841 cgtatctgtt acatcctggc gttacccttg tcgcaaagaa gcacacaaca aggagcaaca
    24901 atggcactgc caattctgtt agattgcgac ccaggtcatg acgacgctat cgcaatagtt
    24961 ctcgccctcg cctcaccaga gcttgatgtc aaagcaatta cgtcttccgc cggaaaccag
    25021 acaccagaaa aaaccttacg caatgttctg cgtatgctga ccttgcttaa tcgcaccgat
    25081 attccggtag caggcggcgc ggtaaaaccg ttaatgcgtg agttgattat cgcggacaat
    25141 gtgcacggcg aaagcggtct ggatggcccg gcattaccgg aaccgacgtt cgcaccgcaa
    25201 aactgtacgg cggtagagct gatggcgaaa acgctgcgcg aaagtgcgga acctgtcacg
    25261 attgtgtcta ccggaccgca aactaacgtt gccttgctgc tcaatagcca tccggaactg
    25321 catagcaaaa ttgcccgtat cgtgattatg ggcggcgcaa tggggcttgg taactggacg
    25381 cctgcggctg aatttaatat ttacgttgac ccggaagcgg cagaaattgt cttccagtca
    25441 gggatcccag tggtgatggc cggtctggat gttactcaca aagcacaaat ccacgttgaa
    25501 gacaccgagc gtttccgcgc gattggcaac cctgtttcaa ccattgttgc cgaactgctg
    25561 gatttcttcc tcgaatatca taaagacgaa aaatggggct ttgtcggtgc accgctgcat
    25621 gacccatgca ccatcgcctg gctgttgaaa cccgagttgt ttaccactgt tgagcgctgg
    25681 gttggcgtgg aaacacaggg gaaatatagc cagggtatga cggttgttga ttattattat
    25741 ctgactggca ataaaccgaa tgccactgta atggtcgatg ttgatcgtca gggctttgtt
    25801 gatttactgg ctgatcgtct gaaattttac gcttaacaca cgtcattgat taagatcacc
    25861 acgcgttacc tgggtggtct taatctgttt atctgtttat ctctttttct ttcaagatat
    25921 aaatgcaatg gataatgcag aactcgccat tggtatcgat ctcggcacta ccaatagttt
    25981 aattgccgtc tggaaagacg gtgccgcgca attaattcca aataagttcg gtgaatattt
    26041 aacaccatcc ataattagca tggatgaaaa taatcatatt ttagtcggaa aaccggctgt
    26101 atcacggcgt acttcgcatc cggataaaac ggcagcgtta tttaaacgtg caatgggcag
    26161 taataccaac tggcggttag gcagcgacac atttaacgcg ccagaactgt cctctttggt
    26221 attacgctca ttaaaagaag atgccgaaga atttctgcaa cgtccgatta aagatgtggt
    26281 gatctccgtt ccggcttatt tcagcgatga acaacgcaag catacccgtt tagcagcgga
    26341 gttagccggg ttaaatgcgg tacgcttaat taatgaaccc acagcagctg cgatggcata
    26401 tggcctgcat acgcaacaaa atacccgctc gctggttttt gatctcggtg gcggcacgtt
    26461 tgacgtcacg gtgctcgaat acgccacgcc ggtgattgaa gttcacgcct ccgctggcga
    26521 taacttcctc ggtggcgaag attttaccca tatgttggtc gatgacgttt taaagcgcgc
    26581 ggatgtcgcc aggaccacgc ttaacgagag tgaactggca gccttgtacg cctgtgtgga
    26641 agcggcaaaa tgtagcaatc aatcgccatt gcacattcgc tggcagtacc aggatgaaac
    26701 gcgggagtgc gaattttacg agaacgaact ggaagatttg tggttgccgc tgctcaatcg
    26761 cttgcgagtg ccgattgaac aggcgttgcg cgatgcacgt ctgaagccga gtcaaatcga
    26821 cagtctggtg ctggttggcg gcgcgtcaca aatgccgctg gtgcagcgaa tcgccgtgcg
    26881 tctgtttggc aaattaccgt atcaaagtta cgacccgagc accattgtcg cgctgggcgc
    26941 agcaatccag gccgcctgcc gcttacgcag tgaagatatt gaagaggtga tcctcactga
    27001 tatttgccct tactcgttgg gcgttgaagt taaccgccag ggcgtttccg gcattttctc
    27061 gccaattatt gaacgaaaca ccacagtacc cgtgtcgcgt gtagaaactt actcgaccat
    27121 gcacccggaa caggattcta ttacggttaa cgtctatcag ggagaaaacc acaaagttaa
    27181 aaataacatt ctggttgaat ccttcgatgt gccgttgaag aaaaccgggg cttatcagtc
    27241 gattgatatt cgctttagtt atgatatcaa cggcttgctt gaagttgacg tgcttctgga
    27301 agatggcagc gttaagtcca gagtcattaa tcatagcccg gtaacattga gcgcgcagca
    27361 gattgaagag agtcggacgc ggttatccgc attgaaaatt tatccgcgcg atatgctaat
    27421 caaccgcacc tttaaggcca aactggaaga gttgtgggcg cgggcgctgg gtgacgagcg
    27481 agaagagatt ggccgggtga tcaccgattt tgatgcggcg ttgcagtcaa acgatatggc
    27541 ccgcgtcgat gaagttcgtc gacgggcgag cgattatttg gctattgaga tcccttaaac
    27601 acggctgccg gatgcggcat aaacgctgca tccggcatcg gcgcaaaaaa cacatcctca
    27661 gctaaacaac atcgcgattt ttccgagcgc ataacctatc atcagcaccg ttaagctgat
    27721 tttgataata ctggtaatgc tctgtggttt ttcatcctca tcattttttt cttcatggtt
    27781 ttcaggtgtt ggctcatcga catcttcatc ctccggctcg agtttcactt tcgtcaacag
    27841 catctgggtg ataatggcct ctaatggatg agcatctcca ccttccagcc ttgtattaag
    27901 acgggccagc atctcttgtt tcgggaacgc ttccagcaca acctgttccg ctacccgata
    27961 caaatccagt aacggtgaat cttgctctgt ctcgctaccg actctcgatt tccaccacgc
    28021 ccatgctttg cgcttgaaga ataacgactc aataaacgat tgacctaacg gcgataattt
    28081 tccgctatgt aaaatctccg cccataacag ccgcgtccgg gcgctctcac caggaacata
    28141 tttcaacggt ttaccttgcg catctaccat gcttcgcgct tcgataacca tcactaataa
    28201 cgcgaaataa tccgggcaat gacgcttgct ccactgcaac aacaaattat cagcttgtgt
    28261 agatgggtaa ctttcccagt acgcgcagag atcggcaagc aggctttccc cctcgccgca
    28321 gtacaaacgt tgtgcacact ggtaataccg ggcatcttcg ctttctggtt cttccgcttg
    28381 ccactgtttg gctacaggaa tgagttccgc catacctgtt tgcaccgagc tgtaccagcg
    28441 caacagtttg cgatgtaact ttgcatcatc aggaatgaac cacggtcctt ccatcgccaa
    28501 aaacgcagtg acatagtcag gatggtaacg gcagcaacgt tccagcgcga agtaatattc
    28561 aacagtctgg ttctgtaacg cgacgggata ttccagaagg ctaaggaaat cgaaacaatc
    28621 accagcctga atggcctcaa gaaaagcctc cctttcttcc tcatccattc cttcgctgtc
    28681 attaaacgaa tgccagttca aacgctctgc caacaaagaa gcacaattta atgaaagata
    28741 tcgcgcttct atagcgattg cacacagcgg ccagcgtaat tgatcgacat cctccatgtt
    28801 ccaggtattt aattgctgaa taaatttctg ccaggcggag ggctgaaaac gatcactttc
    28861 tgaatccagt aatgtcctga atgcacgtag tatttcatgt tcagcggtgg catctttttc
    28921 ttcatcatct gcttcctcga caggatttac cgccagtcgc agcgcctctt cataggcctg
    28981 gcgtaatgct ttaaacccct gcggatcggt ttcgggatga cacaacggta agcgagcaag
    29041 ataagcctgg cggataatgt ctatttgcgt cgtgctttca atttcgagga tttgccaaca
    29101 tgttttcata ggtcgctcac atccttaagc gttaccgggc attcaggctg gacagttaat
    29161 ggttgccagg gaagcgtggc atagaaacgg tcgcttcccg ctgccagctc ttcgaacatc
    29221 cgactcccgg cgctatcgta tttgtacagg gttagcgcct cgcgtagcga agcgttgtct
    29281 tcgcactgag aagattgcca gtagagacgc cctagtgaat aggcagaaaa atagtgcatc
    29341 cagctgtgat agtaatggtg agctcgtgca tagacgcgcg attgcagcca taaactctct
    29401 tcttccgtca gccagttgtt gagcacaccg acccgtgaca gaaaccccat tcgaacataa
    29461 tcccaggctt tgattccccc ttctccacag cacatagcgg tttgggcaac gaattgggta
    29521 taaatcaacc ctctctccga gccaccagca attaatgctt tccactcttc aggggaagag
    29581 cgttgccatg tcagatataa gccagctaaa tcattggcat gaccatcatc agtcatctga
    29641 taaatagtct gaattaaatc atcacgatta tttattcccc aggatgatga gagatcgaat
    29701 tcttttatcc cagtatacat ttttggcgag tcatgcgcgg gtttgcaact ctgattaagt
    29761 atttccatgg gcgcagaaag cgcaaaaaga agat
//
LOCUS       DAKISA010000048        28120 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.048, whole genome shotgun sequence.
ACCESSION   DAKISA010000048 DAKISA010000000
VERSION     DAKISA010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28120
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.048"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            1..317
                     /gene="tnpA"
                     /locus_tag="OVW45_002194"
                     /pseudo
     CDS             1..317
                     /gene="tnpA"
                     /locus_tag="OVW45_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(448..1771)
                     /locus_tag="OVW45_002195"
                     /pseudo
     CDS             complement(448..1771)
                     /locus_tag="OVW45_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(1771..3387)
                     /locus_tag="OVW45_002196"
     CDS             complement(1771..3387)
                     /locus_tag="OVW45_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCV5526630.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQTGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFILGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3683..4399
                     /locus_tag="OVW45_002197"
     CDS             3683..4399
                     /locus_tag="OVW45_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCV5526631.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(4396..6057)
                     /locus_tag="OVW45_002198"
     CDS             complement(4396..6057)
                     /locus_tag="OVW45_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCV5526632.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKDTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(6253..6681)
                     /gene="ibpB"
                     /locus_tag="OVW45_002199"
     CDS             complement(6253..6681)
                     /gene="ibpB"
                     /locus_tag="OVW45_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCV5526633.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(6793..7206)
                     /gene="ibpA"
                     /locus_tag="OVW45_002200"
     CDS             complement(6793..7206)
                     /gene="ibpA"
                     /locus_tag="OVW45_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCV5526634.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(7200..7272)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            7512..7844
                     /locus_tag="OVW45_002201"
     CDS             7512..7844
                     /locus_tag="OVW45_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCV5526635.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(7846..9060)
                     /locus_tag="OVW45_002202"
     CDS             complement(7846..9060)
                     /locus_tag="OVW45_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCV5526636.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYCASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            9161..10225
                     /gene="cbrA"
                     /locus_tag="OVW45_002203"
     CDS             9161..10225
                     /gene="cbrA"
                     /locus_tag="OVW45_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCV5526637.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(10222..11559)
                     /locus_tag="OVW45_002204"
     CDS             complement(10222..11559)
                     /locus_tag="OVW45_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCV5526638.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(11634..12782)
                     /gene="dgoD"
                     /locus_tag="OVW45_002205"
                     /pseudo
     CDS             complement(11634..12782)
                     /gene="dgoD"
                     /locus_tag="OVW45_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(12779..13396)
                     /gene="dgoA"
                     /locus_tag="OVW45_002206"
     CDS             complement(12779..13396)
                     /gene="dgoA"
                     /locus_tag="OVW45_002206"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCV5526639.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(13380..14258)
                     /gene="dgoK"
                     /locus_tag="OVW45_002207"
     CDS             complement(13380..14258)
                     /gene="dgoK"
                     /locus_tag="OVW45_002207"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCV5526640.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFTAGLERGLNTPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(14255..14944)
                     /gene="dgoR"
                     /locus_tag="OVW45_002208"
     CDS             complement(14255..14944)
                     /gene="dgoR"
                     /locus_tag="OVW45_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCV5526641.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            15222..15878
                     /locus_tag="OVW45_002209"
     CDS             15222..15878
                     /locus_tag="OVW45_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5526642.1"
                     /translation="MKLNLKGFFKTAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLKDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYNWSTEEEKARVTQYRFACGVQHCTRTVENLEGTIHEKNKN
                     MDYSNVMAFYHPFKVRFYEYHSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(15924..16736)
                     /gene="yidA"
                     /locus_tag="OVW45_002210"
     CDS             complement(15924..16736)
                     /gene="yidA"
                     /locus_tag="OVW45_002210"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCV5526643.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(16851..17249)
                     /locus_tag="OVW45_002211"
     CDS             complement(16851..17249)
                     /locus_tag="OVW45_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCV5526644.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(17489..19903)
                     /gene="gyrB"
                     /locus_tag="OVW45_002212"
     CDS             complement(17489..19903)
                     /gene="gyrB"
                     /locus_tag="OVW45_002212"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCV5526645.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(19932..21005)
                     /gene="recF"
                     /locus_tag="OVW45_002213"
     CDS             complement(19932..21005)
                     /gene="recF"
                     /locus_tag="OVW45_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCV5526646.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(21005..22105)
                     /gene="dnaN"
                     /locus_tag="OVW45_002214"
     CDS             complement(21005..22105)
                     /gene="dnaN"
                     /locus_tag="OVW45_002214"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCV5526647.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(22110..23513)
                     /gene="dnaA"
                     /locus_tag="OVW45_002215"
     CDS             complement(22110..23513)
                     /gene="dnaA"
                     /locus_tag="OVW45_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCV5526648.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            23810..23890
                     /locus_tag="OVW45_002216"
     CDS             23810..23890
                     /locus_tag="OVW45_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5526649.1"
                     /translation="MTIDKNWLNRSNKDLGRSLRFTHQPV"
     gene            24120..24260
                     /gene="rpmH"
                     /locus_tag="OVW45_002217"
     CDS             24120..24260
                     /gene="rpmH"
                     /locus_tag="OVW45_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCV5526650.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            24277..24636
                     /gene="rnpA"
                     /locus_tag="OVW45_002218"
     CDS             24277..24636
                     /gene="rnpA"
                     /locus_tag="OVW45_002218"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCV5526651.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            24600..24857
                     /gene="yidD"
                     /locus_tag="OVW45_002219"
     CDS             24600..24857
                     /gene="yidD"
                     /locus_tag="OVW45_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCV5526652.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            24860..26506
                     /gene="yidC"
                     /locus_tag="OVW45_002220"
     CDS             24860..26506
                     /gene="yidC"
                     /locus_tag="OVW45_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCV5526653.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            26612..27976
                     /gene="mnmE"
                     /locus_tag="OVW45_002221"
     CDS             26612..27976
                     /gene="mnmE"
                     /locus_tag="OVW45_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCV5526654.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVEVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         6782 a         7585 c         7172 g         6581 t
ORIGIN      
        1 atgacgcgac agaaaaacta cgcacttact tttcaaatgt atgtgctgat tttgaagctg
       61 aactggttta aatggatggc gaaccagatc acgtccattt gttaattaac tatcctccca
      121 aactggcgat atccagtctg gtaaacagcc tcaaaggcgt atcgggtaag ttactgcgac
      181 gagatcgacc agatattgca gtcaggtatt actacaaagg cgttttgtgg agtcctggct
      241 attttgccaa tagctgcgga ggtgcgccaa tatccgtcat ccgccaatac attgaacaac
      301 agcaaacacc tggttaggtg gaaaaccgcg ccttatatcc ccgccctgaa ggacggggtt
      361 ttacggcgca ccggataaat ttggttatgc gccgtcagcc gtgctaaaga atacggacca
      421 gcacccaacg gatgccagtc cacacaatta acgtaactct ggccagtacg ctttgttggc
      481 ctccagcaac tcatccagaa tagctttggc gaccgaggcg ctcggtaccg ttttcgataa
      541 cgtaatcgcc tgccacaggt gctgatatga acgctgttcc caggcgtcca cgaccagttt
      601 ttccaccgcc acttgctgac tcattaaccc tttctgaaac tgcgggatat cgccgaccac
      661 cagtggttct ggtccgttgt gcccgaccag acacgggatc tcgaccatcg cttcatcatc
      721 aaagttatgg attgccccgt tgttaggcac aatcagcaac atccgctcct gagtgttgaa
      781 ggcaattgcc gccgccagat cgacgatgta tgacgcatgt tcgtcaatct ccagcttggc
      841 cgccgctgaa tttcctgccg ctgtaatggc gcggcaagca tcgaaaacct gtttctcgcg
      901 atgttccatt cacctcattc gcgcgggtat gttcagggtt ggaatgctga acaacataat
      961 ccgggaagag ataatatttc agataggtgt tcggcaatgt atccgggtcc agtgcctgga
     1021 catcccgcgc ttttgcatag gtgtcgttcc agctcgcttc agtatgtgta tcttgctgcg
     1081 gtggaacata accgtgcttc gagacatatt gccgcagctg gggcatcagg tcgttgccct
     1141 gcaaatcttc aattgatgtc caccagccaa agtggttcag gccgtagtag cgcacgcgca
     1201 tctgtttgcg atcttgcagc ccaacaattt gcgccatccg gctttcaata ccgattggca
     1261 tgtcacagat gttgaggatt ttcgcattcg ggcgcagacg acgcgtggct tctgcgacaa
     1321 tggctgccgg gttggagtag ttgagcatcc aggcatttgg tgaatatttt tccatataat
     1381 ccaccagttc caggacgccg ccaatggaac gcatgccgta cgctattccg ccgggtccgc
     1441 aagtttcctg accaacaaca ccgtggcgca gtgggatttt ttcatccagt tcgcgcatcg
     1501 ggtacttgcc gacgcggatg tgcgccatga caaaatcaac gtcgctgaac gccacttcag
     1561 gatcggtggt gtaactaaac gcaatgtccg gcgctttctc tttaaggatg actttacagg
     1621 cttcggcaat cacttcctgc cgcgcgccat cgttatcata aaatttcagt gcacgaagcg
     1681 ggaaacgctc ctgattcgcc aggagcatca acacgatgcc tggcgtaaag gtgcttccac
     1741 cgcctgcgac aaccactgag aatttggtca ttatactgcc tccgtaatgg caacattttc
     1801 tgctgattga tgagaattaa ttaagctatc gagctgttca cgcagctggg atacatgcag
     1861 accaatgatc acctgaatgg catcgccact acggaagacg ccgtgcgctc ccagcttttt
     1921 aaagacttcg tcatccagcg tttgtgacat gtcatgcagt gcaatgcgta gacgcgtcgc
     1981 acaattgtta atgctggaga tattgccgac cccgcccagg gcttgcagga taccggcagc
     2041 ctgatccagc tctttttttg gctctgccgc ggtggtttgg cctcgcgagg ctttgtattc
     2101 ggcttttgag tagagtttca cttccgcatc ttcacgtccc ggcgtgcaca tgttgaactg
     2161 cagaatcagg gtgcggaaaa ccacgaagta cagcagggta aagcacaacc caatggcgat
     2221 ttgggtcagc atcatatccg cgtggttgct gaacatcggg atccagtttt gcggtaaaac
     2281 ctggtcaatc agacctccgc ccatgttgcc caccacacca aagagataca tcacggtcga
     2341 cattgaggcc gcaagcacag cgtgtaccgc aaacagcaac ggtgaaatga acaggaaggt
     2401 aaattccagc ggttcggtaa ttcccaccag catggcggtt aaggttgcgg gaatcagcaa
     2461 gcccgccact tttacccgat tttccggtgc ggcagtgaag tacatcgcta aagaaatgcc
     2521 cacggcacca aagatttttg agttaccgtg cagggcaaaa ccgccttccg ggaacaacga
     2581 tttcagcggc tcggcgctca aactgaactc ttgcagatgc tgcgcccagt acatctgaat
     2641 gccgccttca acagctgccg gacccaagat aaacggtccg tagatgaagt ggtgtaaacc
     2701 ggttgggatc agaatacgtt cgaggaaggt gtaaacccag accccaagcg cacccgccga
     2761 acgcaggaac gcttgcagag attcaatccc cgtttgtact tttggccagc cgagcaatgt
     2821 cagccaggca caggggatca tcaccaggaa ggcgataatc accacataag acgtcccctg
     2881 gaaaatgccg agaaaaaccg gcagtttttt atcgaacaga cggttatgca gcgccgtcac
     2941 aatgccggaa atgataattg cgccgataat gctggtatcg agggttttaa tcccggccat
     3001 cattgtcaga ccgctacctg ccaccgcgtc ctgagtgaaa tcgacgccga agtagcttcc
     3061 ccaggtcatt cccatcgcgt tgatgaaata gttccaggtc aggaaactca ccatcaccgc
     3121 cagacaagca cgtccctgcg cttgcttagc aaggccaatg ggtaaaccga cagcaaaaat
     3181 cagcggcata ttacggaata ccgtccaacc gccctcttca ataatgtgta cgatttgcgc
     3241 gaataaactg ttcggatcgg tcagtgattc cccgacaaac atcgggtttt gcagcaagat
     3301 ggcaagaccc accacaatcc cggcgaaggg aaacagcagc actggcgtaa acatcgcgcc
     3361 gccaaagcgt tgaatttgac tgagcatttc cacatcctct tttctcaatt ctgtaagtag
     3421 gaagcctgat tggttatgcg gcaggcctgg agcaaggata ggaagatgcg atcggcagaa
     3481 catgacgacc caatgggatt cgtgatcgct ttcatgcttt tcgttgggcg gtagagatct
     3541 acgttttcgc gaaaaagtag acatctgtga gcacaggtac acagcatcga acacatcttt
     3601 aaaaaaaaga tgttttttca atcgattaag cagaacttgt gtgcgcatta cccgggcttg
     3661 caggcaaaaa agagatctag agatgatcta caaaagcatt gcggagcggt taagaattcg
     3721 acttaactcc gcagatttca cgctaaacag ccttcttccg ggtgaaaaaa agctggcgga
     3781 agagtttgcg gtatcgcgga tgaccatccg taaagccatt gacctgctgg tagcgtgggg
     3841 gctggtggtc cgccgccacg gtagtggcac ttacctggtg cgcaaagatg tactgcatca
     3901 aaccgccagt ctgaccgggc tggtggaggt gttaaaacgg cagggaaaaa ctgtcaccag
     3961 ccaggtgctg atttttgaaa tcatgcctgc gcctccggcc attgccagcc agttacggat
     4021 tcaaatcaac gagcagatct acttctcccg tcgcgttcgt tttgtggaag ggaaaccgct
     4081 gatgctggaa gacagctata tgccggtaaa actgttccgt aatctttcgc tgcaacatct
     4141 ggaagggtcg aagtttgaat atattgaaca agagtgcggg attttgattg gcggtaatta
     4201 tgaaagcctg acgccggtgc tcgccgatag actgctggcg cggcaaatga aggtagcgga
     4261 acacacgcca ctgctgcgga tcacctcgtt gtcatatagc gagagcgggg agtttttgaa
     4321 ttattcagtg atgttcagaa atgccagcga ataccaggtg gagtaccatt tacggcgact
     4381 ccacccggaa aagagttaac cgatactcca gaagagcacc gccagtaatt ggggggtgat
     4441 aatgcgcagg aacatcacta acggatagac agtggcgtaa gagagcgccg ccgcaccgct
     4501 agttggatga agattattag caaacgccag cgccggagga tcggtcatgg aacctgccag
     4561 catcccgcac atggtcaggt aattcatttt ggctaacatc cgcgccagaa tgccaacagt
     4621 aatcagcgga acggcggtga tcagggcacc ataaccaatc cagcttagcc cttcgccatt
     4681 gaccagggta ttcacaaaat ccccaccaga tttcagacca acgaccgaga ggaacagcac
     4741 gatccccagc tcccgcagcg cgaggttggc acttggcggc ataaaccagt acagcttgcc
     4801 gatactgccg atacgcccga ggatcaacgc cataatcagc ggaccgcccg ccagccccag
     4861 tttcaacgcg gccgggaatc ccggcacaaa gacgggaata gaacctaaca atacgccaag
     4921 cccgatgcca ataaacactg gcagcatctg aacctgttgc agtttttgct gcgcattccc
     4981 cagcacattg gcaacggcat caattgcgga cggacgcccc accaggttga ggatatcgcc
     5041 gaactgcagg ctgatatcgc cactggcgac cagttcgacc ccggcacggt tcaggcgcga
     5101 gataacaacg tcatagcgtt ctttaaagtg caggtcgcga atacgttttc cgagcacgtt
     5161 ttcattggtg accaccacac gctcgacgcg caaatcagtg tctttcgtgg acagcgaagt
     5221 atcgacctcc tgaccaatca ccagttgcgc attatgtaaa tccgctggct gacccaccag
     5281 atgcagcaaa tcgcccagtt ggataatggt atctggcgaa ggaactttta gggtttcttc
     5341 gcgtttcaga cgcgagcaga taattttgtc gccgttgaga atcggtacat ctttaatggc
     5401 taaatcatgc aggttagggt tctcaacgcg aatattgata gtcttgatca gcgcgccgcc
     5461 attggtgcgt gaagactcgt gctgctgagc ttctgtctcg acattgacgc ggaaaataac
     5521 ccgcaacatc cacatggtga acaaaatccc gcaaatgccg aatggatacg ccatcgcgta
     5581 actcatcccc atctgatcga ccatttccat tggtgtaccc aggtcgcgca aaatctgctg
     5641 ccctgccccc agcgctggcg tattggtaac cgcaccggag aaaatcccca gcactaccgg
     5701 cagtggaata tcaaacagtt tatgcaggat ggcggtaacc agaccaccga tgatgacgat
     5761 cagaacagca aacaggttga ggcgtaatcc ggagacgcgc aatgaggcaa agaagcccgg
     5821 ccctacctga atcccgatgg tataaacgaa caggatcagg ccaaattcct gaataacatg
     5881 cagcatatcg ctacttaatg tcatccccgc ctgagaaaca aaatggccga cgatgatccc
     5941 accaaacagc acgccgccaa tacctaatcc tatgccgcga aatttgacgt tgccgataaa
     6001 caaaccgacg actgccacca aagccagaat actgaccgtt aatgctatat cactcatcgt
     6061 ccatttctct tgagataaca ttttaattgc tacggattat ggctgaatcg gtgaagcaga
     6121 tttgccttat agcgcacatt attacgggaa agaacgtgcc gaaatatctt aaacagtcga
     6181 aatttaacgc attgttgaag aagaaagaca aaaaaagccc caccagaatg gcggggcaaa
     6241 gagaatagct agttagctat ttaacgcggg acgttcgctg atagcgatac gctgcgctgc
     6301 gatgggttca ggctcattac gaattaaatc aatatgcagt aaaccgttta cgaaagttgc
     6361 gccagagact tccatatttt cagccagcgt aaagctcagg ctaaatggct gattcataag
     6421 cccttgatgc agccattttt tctcttcttt tggctgctcc ggcgtgcctt ttacgctcag
     6481 gcgcgtacct tccagttgaa tctctaaatc ttcctgacgg aaacctgcca gcgcaagggt
     6541 aatgcggtag tggttatcgt cgcttttctc aatgttgtac ggcgggaagc tctgactttc
     6601 accggcgttt tgcagtgcgt tggccagttt gtcaaaaccg atccattgac gcatcagtgg
     6661 ggataaatcg aagttacgca tagtcatttc tccttctaag aagcgagtaa gtacctgcaa
     6721 atccgaagat tcgcatatgc tccctgacgg cgagcatgga gatgtcaggc cgcgccaggc
     6781 ggccttaagg aattagttga tttcgatacg gcgcggtttt ttcgcttccg gaatcacgcg
     6841 ttcgagatcg atatacagca aaccatttac caggttagcg ccacgaacat gaatgttctc
     6901 agctaactgg aatttgcgtt caaagttgcg ttcagcgatg ccctggtaca gataggtgcg
     6961 ctctttttgt tcgtcggcgt gagcaccttt caccaccagc agattatcct gggcggtaat
     7021 ttccagttcg ctctcagcaa aaccagccac agcgatagca atgcggtaat ggttttcgtc
     7081 taccagttca acgttatacg gagggtagcc gccattactc tggctctggt tgttttctaa
     7141 gtggttaaac aaacggtcaa atccaatagc agaacggtaa agcggggata aatcaaagtt
     7201 acgcataatc aatagctcct gaaatcagcg agaatgtaag accttccaca atggacaggt
     7261 caggtagcca gaacacccat caggcgcgtc ctcatcgact acgatgtaaa aatgggtctg
     7321 gaaatgaact tttcaagcct tatcagcgga cttttttttg cagtttatgg tctattgcat
     7381 agactgaggg ggcagcacga aacgttaaag tgcgatagcc gccacagagc gacgtaatga
     7441 tgatgttatt ttcagcaagg gatcgctata actcatcagg ccaacaccca cgatcaacag
     7501 atgaatttat tatgataaga aatgtgttgt tagcgttcat gatatgcagc ggaatggcat
     7561 tactcggagg atgctccagc gtgatgtccc ataccggagg gaaagaagga acgtatcccg
     7621 gcacccgcgc cagcgccacc atgattggcg atgatgaaac caactggggc actaagtcgc
     7681 tggcgattct cgatatgccg tttaccgctg tgatggacac gcttttatta ccgtgggatg
     7741 tgttccgcaa agacagctcg gtgcgctcac gcgttgaaaa aagcgaagcc aacgcgcaag
     7801 ccaccaatgc ggttattcca ccggccagaa tgcctgataa ctgatttagc gatcagtttc
     7861 ggtgatccac agctggccac cttccatcca cgccagccat tgaccatccg gcgagaagac
     7921 cacggcgtcc gcagaaggtg gattggcgtg gttttcggtt aaatactcaa cctcgccgct
     7981 ttgcgcatgg gcacaagcaa ttcgattatc cagcacaaag cccaaccatt ctcctgacgg
     8041 atgccagtta aatgcagact gaatatccgt tttgttatgg gttaactggc gcggctcgcc
     8101 gccctgtggc gagataagcc acagttgcac aatgccgtta tcatcacgca ttaaaaacgc
     8161 gatttgcgta ccctgcggat tacagcgcac ccagtggcgc gggacgttga ctaaccccgg
     8221 ataagcccga tggtgggtaa aggttaaacg tcgctgcacg acgccacgcg gtggcgcggg
     8281 cagggtggtt tccgttccac ttaacggcgc atcacctgcc gctttccagc cagcttcatc
     8341 ttgcggcaac tcaacgataa acagctccgg cactttctca ccctttggcg aaagtgtgtc
     8401 gccaataaac gccagcgcgt gatttcctac ccatccttct tcataagcac gattgatttc
     8461 atcactgcca ggctgcggcg tgggcgtggt tttactcacc agcacacacc agtggctgcc
     8521 gctgtattca cgcggatgct gcttttgtac gttgaccgga ccaaacggcg cagcaacacc
     8581 gacgtttcgc aaatccagcg ccggatcgag ttcatgcatt acatggtcgt tataggtaaa
     8641 gctcaccctt tcaccgttcg ggctaaagac atgcacatgg ctgccgccgc gcagcgctcc
     8701 tggcgtatac ggcgcggtaa tatccattgc atcgagattg ctcaccttgc cgccttcagc
     8761 aatgactccg cgccgatgat ggaagtcgta atgccatgtt tcatcaggat tttccgggcc
     8821 gtgaataaag acatatttct ctgacttcgg atgaacggtc accacgccga cgtgtgcgcc
     8881 ctgtgacgca cagtagataa cctcgacctc accggtatgg atattcacac gctcaatggt
     8941 ctcgccggta aacgacgccc cagaaggacg cacgtcaaat accagccact ggctgtcggg
     9001 cgtccaggta ttggtattgg tgagcaggtg attacgggga gcaaaggtta tctgtttcat
     9061 ggcgcggtcc tgatacaaaa cgggtccagc catcatactt cacaaggact tcactttcag
     9121 acaatagtct tttgccatcg tgatgaatgt gagtgaaaag atggaacatt tcgacgtggc
     9181 gattattggc ctcggcccgg cagggtcggc gttggcacga aagttagccg gcaaaatgca
     9241 ggtgatcgcg ctggataaaa agcaccagca tggtactgaa ggtttcagca aaccttgtgg
     9301 cggtctgctg gcaccggacg cgcagcgatc ttttattcgc gatgggctga cgcttcctgt
     9361 tgatgtgatc gctaatccgc agattttcag cgtcaaaacc gtcgacgtcg ccgcatcgct
     9421 cacgcgtaac taccagcgaa gctatatcaa tattaatcgc catgctttcg acttgtggat
     9481 gaaatcgctg atccccgcca gcgttgaggt ttatcacgac agcctgtgtc gaaaaatctg
     9541 gcgtgaggat gataaatggc atgtcatttt tcgtgcagac ggctgggagc agcatattac
     9601 cgcccgctat ctggtcggtg ccgatggtgc caactcgatg gtgcggcgac atctctaccc
     9661 ggatcatcaa atccgtaaat atgtcgcaat ccagcagtgg ttcgcagaga aacatccggt
     9721 gccgttctac tcctgcatct ttgataatgc gataactgac tgttattcat ggagtatcag
     9781 caaagacggt tattttatct ttggcggtgc ctatccaatg aaagacggtc agacgcgttt
     9841 cacgactctg aaagagaaaa tgagcgcctt tcagttccag tttggcaaag cggtgaaaag
     9901 cgaaaaatgc acggtgctgt ttccctcacg ctggcaggat tttgtctgcg gtaaggacaa
     9961 cgcctttctg attggtgaag cggcgggatt tatcagcgcc agctcgctgg aggggattag
    10021 ctatgcgctg gatagcgcag acattctgcg ttcggtgtta ctgaagcagc cagagaagct
    10081 caataccgct tactggcgcg ccacccgcaa actgcgttta aaactcttcg gcaagatagt
    10141 aaaaagccga tgcctgaccg caccggcttt aagaaagtgg attatgcgca gtggtgtggc
    10201 acatattcca cagttgaagg attagccaac gcgcttcaca tcgcccacca gcaggatata
    10261 agagagcgcg ccaatcaacg cgacggcgga gatataaacc agtgcaggtg cgaaaccgta
    10321 accctgcgcc aggtacccca ccaccagcgg aacggtgatg ccgcccagac caccggcgaa
    10381 gttaaacacg ccgccggtta aaccaatcag gcgcatcggt gccagagaag aaaccagcga
    10441 ccaggtaatc gaagcaaaac cgttaccgaa gaatgccagc gccatcaggc acataatcat
    10501 catcggatcg ttagtgtagt tagcgcccat aatgcaggtg gagatcagca agccgcagat
    10561 aatcggcgtt ttacgcgcaa agcccagtga aaagccctta cgtaccagca gatccgctac
    10621 ccagccagag agcaggacgc cgacaaacgc cgcgaggaat ggcaccgtgg tcataaagcc
    10681 tgctttcagc gccgtgattc ctttttcctg ggttaaatag ttcgggaacc aggttaagaa
    10741 aaaccacagt gtagaagcca ccgcaaattg cccaagatag acgccgatca gtttacgatg
    10801 gaacaccagt ttccagtctt tggctgttaa cggctgacgt gcctctttct tcactggcgc
    10861 atcaccatcc accagaccgc cgccatcacg aatgtaatcc agttcagctt tgctgatgcc
    10921 tttggtcagg cgcggcggct gataaacctt aaaccaaatc agcgaccaga taatgccgat
    10981 accaccagtc acaatgaaca cccagtgcca gctcaacatc tcctgaatcc agatcagcag
    11041 cggagtcaaa aacgccagac cgacaaactg accagacgta taaaaaccaa cggcggaagc
    11101 gcgttcatgt tccgggaacc agctggtcac catccggtta ttggtcggga aagcgggcgc
    11161 ttcgaaaata ccggttatcg cacgcagacc aattaatgac attaatcccg tggcaaagcc
    11221 ctggaataaa gtcgccactg accagccaaa tatcgcaata aaataagtca cgcgagaacc
    11281 tacgcgatct aaaaaccaac cgccggggat ctggcatagc gtataaagcc aggcgaaggc
    11341 cgaaaataca tagcccattt ccgctttcgt aatgccgaac tcttcctgaa tatgggcgga
    11401 agccacggcc aggttagcgc ggtcaacata acaaatgacc accgtaataa agatcatcac
    11461 cagcgtcaga taacgccgac gccccggctt tgctgcatta acgggaatat ccatagcgag
    11521 ctttctccag attttgggca tagcgaagcc gctcaccatg ccctgtaatt tacagagggt
    11581 tatatttttg tattgctgtt ttagtgcccg atgaggggct tacgtggcag gaattaccac
    11641 tctgctacgc tgttatcttc atgacgccag agtggattac gccagtccgg ggcattttta
    11701 ctgaactcaa taactttagc ttcgtcgatt tccacgccta agcccggttt cgttagcggt
    11761 ttaaagaagc cgccaaccat gctgaagtct tctttgtttt tcacaaagtc gagtaactcc
    11821 gcgcctttgt tgtaatgaat tcccatactt tgttcctgaa gtaccgcgtt ataggaaaca
    11881 aagtcgatat gcagacaagc cgccagtgca atcggtccga gcggacagtg cggcgcaagg
    11941 gtcacgtcat aggcttctgc cattccggca attttgtagc attcggtaat accgcctgca
    12001 tgggagagat ccggttgtag aatcgaaata ccgcctgcct ccagcacgcg tttaaaatcg
    12061 aaacgtgaga acatgcgctc acccgccgcc agtggaatat gcgtttgtgc cgccaatttc
    12121 gggtagtatt cggcttgttc cgccagcacc ggctcttcaa taaacagcgg gcgatacggc
    12181 tccagttctt taatcagcac tttcgccatt ggcgcgctga cgcgaccgtg gaaatcaaga
    12241 ccaaactcaa tctgattgcc aaaagcttca cgaatttgtg ccacggtgtt gactgccgca
    12301 tctaccgcgc gggagttatc aattagcccc agttcttcac aaccgttcag tttgaaggta
    12361 tcgaagccga tttcgcgcag ggttttaata ccgtcgatca catccgccgg acgatcgccg
    12421 ccgacccaac tgtaggcttt aattttgtcg cgaaccaggc cgcccatcag ttgccagacc
    12481 gacgcattca gcactttacc tttgatatcc cataacgcct ggtcgatccc ggcaatggcg
    12541 ctcatcagga ttggaccgcc acgataaaat ccggcgcgat acatcacttg ccataagtca
    12601 ttgatgcgct atggatcctg accaatcaaa tagtcaccca gctcgtgaac tgccgcttcc
    12661 accgtgcggg cgcggccttc aatcactggc tcgccccagc cgacgacgcc ttcatcggtt
    12721 tcaattttca ggaacatcca gcggggaggt aaacgatacg tggtaatttt ggtgattttc
    12781 attgcactgc ctctcgatac gccttaacaa atgctgctgc ctgctgcgcg gtgcgttcta
    12841 cggactgccc ggcgcgatag agatcgctgc ctaagcccgc cccagcacaa cctgcgtcta
    12901 tccactgcgc caggttttct ggcgtcacgc cgccaacggc aaagactgcg atgtccgatg
    12961 gcaataccgc ttttaacgct ttgatgtatt gcggaccaaa agccgatgac ggaaatattt
    13021 tcagcgcctg cgcgcccgct tcgagcgcgg taaaggcttc ggtcgccgtc gcgcagccgg
    13081 ggcagacggt catgccgtag cccaccgcac ggcggatcac ttcactatgg atattgggcg
    13141 taacgatgag ctgacagccc atcctggcga gcgcatcgac ctgttcaggt ttcagtaccg
    13201 tacctgcgcc aatcaacgcc ttgtcgccat acgcatcaac gatggcggga atgctttgct
    13261 cccattgtgg ggaattcagc gggatttcaa ccgcgtcgaa cccggcgtca atcaccgcgc
    13321 caacatgcgc cagcgcctcg tcgggcttaa tgccgcgcaa aatggcgatc agcggaagtt
    13381 tagtttgcca ctgcatgagc gatgctcctt ataccagcct gaaatgccgt gtcgcccgcc
    13441 accgccgtca cgtcgcaacc catcgcctga aaggcttgct ggtagcgcgc ggtcaacgat
    13501 gttccggcga caagggtgat ggcgtgttga tgggccacat agtcgcgcat actggcgacc
    13561 tctgcgccaa tcaacaaacc agagagaaat tcactgacct gttcacgggg aagtgttccc
    13621 agcacatgcg aggcacgaac ttcaaaaagc tgcggcaata tggcgggcgt attaagacca
    13681 cgctcaaggc cagccgtgaa ggcatcggca gagttttcct gcggcggcaa acctgcacca
    13741 atcaatgagt gatttaacag taaatgatgt aattcaccgg tcatcacggt gcgaaaatcg
    13801 ttgatttgct ggctatcggc ctgcacccat ttgcaatgcg taccgggcat gacataaaga
    13861 gaggaaggag ccagagctcg cgcgccgatc aattgagttt cttcgccgcg catcacattg
    13921 tggttatcat catgagagac gcataatccg ggaataatcc agatattgtc gccaacagac
    13981 gttaattgtt cgccaataga cgaaaaacgg gcaggaacag ataaatacgg agcaactttc
    14041 cagccgacat tgctgccgac cattcctgcc attaccactg gcgtattctc ttcacgccag
    14101 tcggtcgtga cttctgctaa caccgcagcc ggagattttc cgttcaggcg cgtgacgcct
    14161 gcttctgatt gcctgctctc caggcagtgg tcgccctgat aaagccaggc gcgcagattg
    14221 gtcgatcccc agtcaattgc gatgtagcga gctgtcatgt gatttccttt aaccttcgtg
    14281 tcgagctggc gatcatggta agcgccgcct gctctgccgc atcgccgtcc tgatgccgta
    14341 tcgcatcgaa cagcgcctta tgttcctgga gcgtttgcgg catgttggcc tcatcgccca
    14401 tccaggttcg ttcaaaaacc gcccgctgca gcgaactgat cgcaatgcta agttgctgta
    14461 acaccgggtt atgcaccgac tgcagcaccg cctcgtggta gcgaatatcc gcttcgttaa
    14521 acgcttcgcg gtcctgattg ttggcaatca tctcgttcag cgccgattca atctgcgcca
    14581 gatcgctgga ggtcgcgcgc tctgctgccc aacgggcaat cgccggttcc accagatttc
    14641 gcacttcgct catggcactg ataagccgtg ggtcgtagtc gttttccagc acccattgca
    14701 gtacgtcagt gtcgaggtaa ttccactggt tacgcggtgc cacaaacgcg ccgcgataac
    14761 gtttcatttc aatcagccgt ttcgccatca acgaacggaa cacctcacgg atgatgttgc
    14821 gcgaggttgc aaactcctca cagagttccg cctcagccgg aagcggcgag cctggtacgt
    14881 atttgccgtg aacgatctgt ttacccagcg taatgacgat gcgatcggtt ttattgagag
    14941 tcatggagag tccttgtgct ctgtatgttc ttctctactt taccccgatc gatgcataac
    15001 acggcaactt tgtagtacca gcgtgatgac gttcgcgttt gctgtgcgtg taatgtagta
    15061 caaacttata ttgttgtact acaatttaga tcacaaaaag aacaatgcgt aaaaaaatga
    15121 catgcgtcgg gcagaaatct gaaaagggat atcaggcgct aaacaggagg gaaagaagag
    15181 tatgctttca acggcttagc tactcgttta aaggattaat catgaagttg aatcttaagg
    15241 gattttttaa gactgccggt ttattcccac tggcgctgat gctttcgggc tgtatctcgt
    15301 atgccctggt ttcccatacc gcaaagggta gttcaggaaa gtatcaatcg cagtcagaca
    15361 ccatcacggg gctatcgcag gcaaaggata gtaatggaac aaaaggctat gtttttgtag
    15421 gggaatcgct ggattacctt atcacagatg gtgccgatga catcgttaag atgctcaagg
    15481 atccagctct taaccggcac aatattcagg ttgctgatga cgcaagattt gttttaaatg
    15541 cggggaaaaa gaaatttacc ggcacaatat cactctatta caactggagc acagaagaag
    15601 aaaaagcacg agtgacacaa tatcgttttg cttgtggtgt tcagcactgt acccggacag
    15661 tagaaaacct ggaaggtact atccatgaga aaaataaaaa catggattac tcaaacgtaa
    15721 tggctttcta ccatccattt aaagtgcgat tttatgaata ccattcaccc agaggcattc
    15781 cggatggtgt ttccgcagca ttactgccag tgactgttac gctggacatc attactgcac
    15841 cgctgcaatt tctggttgta tatgcagtaa accaataatc agtaagcggg caaacgcgtt
    15901 tatgctgttt gcccgcccac agattaattc agcacatact tctcaatagc aaacgccacg
    15961 ccatcttcaa ggttagattt ggtgacaaag ttcgccactt ctttcaccga aggaatagcg
    16021 ttatccatcg ccacaccgac gcctgcatat tcaatcattg cgatgtcgtt ttcctgatcg
    16081 ccaatcgcca tgatttcttc cggtttaata cctaacacgt cggccagtga tttcacaccc
    16141 gtacctttgt taacgcgttt atcgaggatt tcgaggaagt acggcgcact tttcagcacg
    16201 gtatatttct ctttcacttc ctgcggaata cgcgcgatag cctggtcgag gatggcgggt
    16261 tcatcaatca tcatcacttt caggaactgg gtattggggt ccattttctc cgcttcgcag
    16321 aacaccagcg gaatagtggc aacgaaagat tcatgcactg tgtagtagct gatatcgcgg
    16381 ttggcggtgt acagcgtggt gcggtccagg gcgtggaaat gagaaccgac ttcgcgagag
    16441 agtttttcca ggaaacgata gtcgtcatag ctgagagcag tttgcgccac ggtgctacca
    16501 tcagcggcct tctgtactag cgcgccgtta taagtaatgc agtagtcgcc cggctgttcc
    16561 atatgcagct ctttcaggta gttgtgcaca cctgcatacg ggcgacccgt cgttagcacg
    16621 acattcacgc cacgggcgcg agctgcggca atcgcatttt taacggcggg tgaaatggtg
    16681 tgatcgggca gcagaagggt gccatccata tcgatagcaa tgagtttgat agccatgagt
    16741 tccccaggta gattggttcc tgacccatgc taacgcgatt ccgctcaaaa atcagtacaa
    16801 cacccgaggg aaaaggggga tgcaacgcgg gttgcatgct ccccatttgc ttagcggaag
    16861 agtttccctt tcagcagttc catacctgcg gaaagcagat cgttattggc ttgtggtgac
    16921 acttcacctt gcggtgagag cgcatcaata atcttcggca attgctctgc cagtaaactg
    16981 gaagctgtac tggtatccac gccaagtttt tgcccgagat cggacaccgc atttgtgccg
    17041 agcgccgatt ccagttgctc gccactaacc gattgattgc cctgttgatt actcagccag
    17101 gttgagagaa tggcccctaa gccgccactt tgcagttttt ccagcagcac ctgaatgccg
    17161 ccctgctcct caacccaact taaaatagcc tgatatttcc ccgcatcgcc tttcagaaag
    17221 gcaccgacaa cttcatcaaa aagccccatg ataatcacct gtaaagcgtt acgtgttgac
    17281 ccaaaaagta tagatttgcg ggatgataat tgcggattgc agaaataaaa agggcggaga
    17341 tgatctccgc ccttttctta tagcttcttg ccggatgcgg cgtgaacgcc ttatccggcc
    17401 tacaaaatca tgaaaattca atacattgca agattttcgt aggcctgata agcgtagcgc
    17461 atcaggcacg ctcgcatggt tagcgccatt aaatatcgat attcgccgct ttcagggcgt
    17521 tctcttcaat aaacgcacgg cgcggttcaa cggcgtcgcc catcagcgtg gtgaacaact
    17581 ggtcggcagc aatcgcatct ttaacggtaa cgcgcagcat acgacggctt tccgggtcca
    17641 tggtggtttc ccacagctgt tccgggttca tctcgcccag acctttataa cgctggatgg
    17701 agaggccgcg acgggactct ttcaccagcc agtccagcgc ctgctcgaag ctggctaccg
    17761 gctgacgacg ctcgccacgt tcgataaacg catcttcttc cagcaagcca cgcagtttct
    17821 cacccagcgt gcagatacga cgatattcgc caccggtgat aaactcgtga tccagcggat
    17881 agtcagtatc cacaccgtgg gtacgcacgc gaacaatcgg ttcaaacagg ttttgttcgg
    17941 cattggtgtg gacatcaaac ttccactggc tgccgtgctg ttctttgtcg ttcagttcgc
    18001 tgaccagcgc gttcacccag cgggtaacgg tctgctcatc agagaggtcg gcttccgtca
    18061 gcgtcggctg atagataagc tctttcagca ttgctttcgg ataacgacgc tccatgcggt
    18121 tgatcatttt ctgcgtcgcg ttgtactcag acaccaattt ctctaacgct tcgccagcca
    18181 atgccggtgc gctggcgttg gtgtgcagcg ttgcgccatc cagcgcgata gagatctggt
    18241 actgatccat cgcttcgtcg tctttaatgt actgttcctg cttgcctttc ttcactttgt
    18301 acagcggcgg ctgagcgatg tagacgtgac cgcgttcaac gatttccggc atctgacgat
    18361 agaagaaggt caacagcagc gtacgaatgt gcgagccgtc gacgtccgca tcggtcatga
    18421 tgatgatgct gtgataacgc agtttgtccg ggttgtactc gtcacgaccg ataccacagc
    18481 caagcgcggt gataagcgtc gccacttcct gagaagagag catcttatcg aagcgcgctt
    18541 tctcaacgtt gaggatttta cccttcagcg gcagaatcgc ctggttcttg cggttacgcc
    18601 cctgcttcgc agagccgccc gcggagtccc cttccaccag gtacagttcg gaaagcgccg
    18661 gatcgcgttc ctggcagtct gccagtttgc ccggcaggcc cgctaagtcg agcgcacctt
    18721 tacggcgggt catttcacgc gcgcgacgcg ccgcttcacg ggcacgggca gcatcgataa
    18781 ttttgccaac cacgattttc gcgtcggttg ggttttccag caggtattct gccagcagtt
    18841 cgttcatctg ctgttcaacc gctgatttca cctcagaaga aaccagtttg tctttggtct
    18901 gggaggagaa tttcgggtcc ggcactttca cggaaacgac cgcaatcagg ccttcacgcg
    18961 catcgtcacc ggtggcgcta actttggctt ttttgctgta gccttctttg tccatgtagg
    19021 cgttcagggt acgggtcatc gccgcacgga agcctgccag gtgagtaccg ccgtcacgct
    19081 gcggaatgtt gttggtaaag cagtagatgt tttcctggaa gccatcgttc cactgcaacg
    19141 ccacttcgac gccaataccg tctttttcag tggagaagta gaagatattc gggtggatcg
    19201 gcgttttgtt cttgttcaga tattcaacga acgccttgat gccgccttca tagtggaagt
    19261 ggtcttcttt gccgtcgcgc ttgtcgcgca gacgaatgga aacgccggag ttgaggaacg
    19321 acaactcacg cagacgtttc gccagaattt catattcgaa ctcggtcaca ttggtgaagg
    19381 tttcgaggct gggccagaaa cgcaccatgg tgccggtttt ttcagtctcg ccggtaaccg
    19441 ccaacggtgc ctgcggtaca ccgtgttcgt agatctgacg gtgaatttta ccctcgcgct
    19501 ggataaccag ctccagtttt tgcgacaggg cgtttactac cgaaacacca acgccgtgca
    19561 gaccgccgga cactttatag gagttatcgt caaatttacc gcctgcgtgc agaacggtca
    19621 tgatcacttc cgccgccgat acgccctctt ccgggtgaat accggtcgga atgccgcgcc
    19681 cgtcatcctg tacagagaca gagttatcgg cgtgaatggt gacgataatt tctttacagt
    19741 gacccgcgag cgcttcgtcg atagcgttat ctaccacctc gaataccatg tggtgcagac
    19801 cggtgccgtc atccgtgtcg ccgatataca tacccgggcg cttacgcacc gcatccagcc
    19861 ctttcaggac tttgatactg gaggagtcat aagaattcga catcaacgtt tctcgctcat
    19921 ttatacttgg gttaatccgt tattttaccc ttttccacgg taaacatctt cgaattttcg
    19981 tccgacatgt ctataacgtg ttcagcactg atcgcgctga caaagacctg tgattgcgtc
    20041 gcttttaagc ggctggcaag cagcccgcga cgctcatcat caagctcaga ggcaaaatca
    20101 tctatcaggt agagacaccg ccgcccgctt tcacgggtga ggaactctcc ttgcgccaga
    20161 cgtaaggcgc acatcaacag cttaagctgc ccacgcgata aggtatcttc caccggcgca
    20221 ccgtcggcgc gaatgcgtaa gtccgcttta tgcgggccat gcgcggtgta ggttagctgg
    20281 cgatcgcgtt caaaattacg ttccagcacc tcagcatatt ctgtctcttt ctcccagccg
    20341 cgctggaaag agaaagtcag agaaaactca gggagaaatt gcttacaagt atcggccata
    20401 tcagccgcga taccggcgct atactccgcg cgccaggtgc tgatttgctc cgccagcggg
    20461 atcagctctt tatcccacgg gcgtagctgt tcgtaacgtg tcacctggcg cagcgccgca
    20521 ttgcgctgct tgagcaatcg cttgagattg ctccaggcgg tgaaaaatcc gggttcgttg
    20581 tgaaagcatc cccagtcgag gaatgctctt ctgtatttgg ggccgccgtt gagtaaagta
    20641 aacccttctg gcgttatcaa ctgcattggc atcaggtgcg ccagttccgc gaccttatgc
    20701 ccgtctgtac cgtcgatgcg gactttgctg tcgccctgtt tgtctttggt taagccaatc
    20761 gctgtctcgc gctcttcgcc ctgtaatcgc ccgtggagaa caaacgcctc ctgctcatgg
    20821 cgaatgacgc gaccaatctg caaactgcga aacgcccgac catggccgag cgtatagatg
    20881 gcttccagca cgctggtttt gccactgccg ttggcaccta ccagaaagtt aaagccggga
    20941 gataaagcga gatccgcggt ttcaatgttg cggaaatcgc ggatcaacaa gcgggtgagg
    21001 gacattacag tctcattggc atgacaacat aggcagccga ctgtgatgcg gcatcttcaa
    21061 tctgcacgct ggaaaccgaa tcggtcagca tcatgcggac gttttcacat ttcagcgcgt
    21121 tcagaacatc cagcacgtag ctgacgttga agccgatttc catctccgca ccgctatagg
    21181 taacgtcgag gatctcttcc gcttcttcct gttccgggtt gttggcggtg attttcagct
    21241 ggttttcgct gacataaaga cgtacgccgc ggaatttctc gttagagaga atcgccgcgc
    21301 gagcaaacgc ctgcttgagc agatcgcagc cagcttccag atgtttgtcc gggttcttcg
    21361 gcagaacgcg gcgataatcc gggaagcgac catccaccag tttggaggtg aagataaagt
    21421 cgccaacgtg ggcgcgaatg ttgttgctgc caatctgtac gcgcagcgga ttgtcgccgc
    21481 cgtcgagcat acgcatcagt tcaatcacgc ctttacgcgg cacgatcacc gaatggcttg
    21541 gcaaagattg accaattggc attgaacaga ctgccagacg gtggccgtcg gtcgccacgg
    21601 tgcgcagttc ttcgccttcg gtttcaaaca gcataccgtt taagtaatag cgaacgtcct
    21661 gatgcgccat agaaaactgg gtcgcttcaa tcagacgctt catcgtcgcc tgcggcaggg
    21721 taaattcgac ttcgctctgc cagtcatcga ggttcgggaa atccgccgct ggcagggtag
    21781 acagcgaaaa acggctacgc ccggagcgca ccagcatccg ttcaccttcc agctgtacgg
    21841 caatttccgc gccttcaggc agaccacggc agatatcaaa gaatttgcgc gccggaacgg
    21901 tcgtcgctcc tggctcgtgt ggctgaacca gcgcaacgcg tgccaccatt tccatctcga
    21961 gatcagtacc ggtcagcgac aacgtaccgt cagcaacctg taacagcaga ttaccgagaa
    22021 tcggtagcgt aggacgacca cctaacggcc cgctcacctg ttgtagcggt tttaataaat
    22081 gctcacgttc tacggtaaat ttcataggtt tacgatgaca atgttctgat taaatttgaa
    22141 aaatcttctt tgatatcgtg gctctcttca cgcaactgct cgatcttacg gcaggcatga
    22201 agcaccgtcg tgtggtcacg accaccaaac gcatcgccaa tctccggcag actgtggtta
    22261 gtcagctctt tcgccagcgc catcgccatc tggcgcggac gcgccaccga gcgggatcga
    22321 cgcttggaaa ggagatccgc gactttgatc ttgtagtact ccgccaccgt cttctgaata
    22381 ttgtcgatgg tgaccagttt ttcctgcaat gccagcaagt cgcgcagtgc ctcacgcacg
    22441 aagtcgatgg tgatcgcccg tccggtaaag ttggcgttag caataacgcg gttcagcgcc
    22501 ccttccagct cacgtacgtt agatcgtaga cgcttggcga taaagaacgc cacttcgcct
    22561 ggcaaacgaa tgtcattttc gtcggccttt ttcatcagga tcgccacgcg ggtttccagc
    22621 tctggcggtt cgatcgccac agtcagtccc caaccgaagc gggatttcaa acgatcctca
    22681 acgccgttga tctctttcgg atagcgatcc gaggtgagaa tgatctgttg attaccttcc
    22741 agcagggcgt tgaaggtgtg gaaaaactct tcctgagatc gttctttatt agcaaaaaac
    22801 tgaatatcgt cgatcagcag tgcatctacg gaacggtagt agcgtttaaa ctcttcgatc
    22861 gcgttgtttt gcagggcttt aaccatgtcc tgaacaaagc gctcggagtg catataaacc
    22921 actttggcat tcggcttgcg cgccataatg ccgttaccca ccgcatgcag caggtgagtt
    22981 ttacccagac ccgtgccgcc ataaaggaac aatgggttat aggcaccgcc agggttatcc
    23041 gccacctggc gagccgccgc gcgcgccagt tggttagatt taccttcaac gaagttatca
    23101 aacgtgtgtt tgacgtttac gttagaacga taggtcggtt ctgccggggc cgggacgtta
    23161 tcccaacctg agcgcgtaga aggcgcagca cgttgcggct gcgtttgcgc cacctgtgca
    23221 ggggccgcga cgttgctcgt cactgccgct tgtggcgttt gcgtcaccgg tttggtgccg
    23281 acttcaaaac gcagctgtgg ggcatccgct ccgcagaaac tggttagcag tccattgata
    23341 ttattaaggt acttgtcccg tacccaatcg aggacaaaac ggtttggcgc gtacagggcc
    23401 agcgtgttat cgctcagttc cgcctgcaat gggcgtatcc acatactgaa ttctgtggct
    23461 ggtaactcat cctgcaatcg ggcaagacac tgctgccaaa gcgaaagtga cacggcggac
    23521 tccactcgaa caaaagtcga taatgactaa ggctgaaaca ttcatgattg ttgacgtacg
    23581 tcgaaaagac cctgcttgag ggtgacgcac gaaccgctgt ctgcggttat atgcccgatc
    23641 aagatcctgc aaaacgatcg ggaccgcgga tcatagccta aactgcgcaa gagatcttct
    23701 gtttctcaca gattttcccg atttatccac aggactttcc agacctcgct aagtgtaaac
    23761 gatcctgccg cgaggcgggc acgatttacg ccgcatattg gaaaatttaa tgaccataga
    23821 caaaaattgg cttaatcgat ctaataaaga tctaggacga tccttgcgct ttacccatca
    23881 gcccgtataa tcctccaccc ggcgcgccat gctggttttc actggtgtga ggtcgtacat
    23941 tttccctgcg aaaaggtgcg gaaaagcgcg gtaaataagg aaagagaatt gactccggag
    24001 tgtacaatta ttacaatccg gcctctttaa tcacccatgg cttcggtgtc catcgtttca
    24061 tttttcggcg gatatccaat aaagccattg aatttattca agtttaggta gaaatcgcca
    24121 tgaaacgcac ttttcaaccg tctgtactga agcgcaaccg ttctcacggc ttccgtgctc
    24181 gtatggctac taaaaatggt cgtcaggttc tggcacgtcg tcgtgctaaa ggccgcgctc
    24241 gtctgaccgt ttctaagtaa taaagctaac ccctgagtgg ttaagctcgc atttcccagg
    24301 gagttacgct tgttaactcc cagtcaattc acattcgtct tccagcagcc acaacgggct
    24361 ggcacgccgc aaattaccat tctcggccgc ctgaattcgc tggggcatcc ccgtatcggt
    24421 cttacagtcg ccaagaaaaa cgttcgacgc gcccatgaac gcaatcggat taaacgtctg
    24481 acgcgtgaaa gcttccgtct gcgccaacat gaactcccgg ctatggattt cgtggtggtg
    24541 gcgaaaaaag gggttgccga cctcgataac cgtgctctct cggaagcgtt ggaaaaatta
    24601 tggcgccgcc actgtcgcct ggctcgcggg tcctgatagc cctcattcgg gtctatcaac
    24661 gcctgattag tccgctactc gggccgcatt gtcgtttcac tccaacctgt tcaagctacg
    24721 gaattgaggc attgcgcagg tttggagtga taaaaggcag ttggttgacg gtgaaacgcg
    24781 tattaaaatg ccacccttta caccctggtg gtgacgatcc cgtcccgccc ggaccatttg
    24841 ataccagaga acactaacga tggattcgca acgcaatctt ttagtcatcg ctttgctgtt
    24901 cgtgtctttc atgatctggc aagcctggga gcaggataaa aacccgcaac ctcaggccca
    24961 acagaccacg cagacaacga ccaccgcagc gggtagcgcc gccgaccagg gcgtaccggc
    25021 cagtggccag gggaaactga tctcggttaa gaccgacgtg cttgatctga ccatcaacac
    25081 ccgtggtggt gatgttgagc aagctctgct gcctgcttac ccgaaagagc tgaactctac
    25141 ccagccgttc cagctgctgg aaacttcacc gcagtttatt tatcaggcac agagcggtct
    25201 gaccggtcgt gatggcccgg ataacccggc taacggcccg cgtccgctgt ataacgttga
    25261 aaaagacgct tatgtgctgg ctgaaggtca aaacgaactg caggtgccga tgacgtatac
    25321 cgacgcggca ggcaacacgt ttaccaaaac gtttgtcctg aaacgtggtg attacgctgt
    25381 caacgtcaac tacaacgtgc agaacgctgg cgagaaaccg ctggaaatct ccacctttgg
    25441 tcagttgaag caatccatca ctctgccacc gcatctcgat accggaagca gcaacttcgc
    25501 actgcacacc ttccgcggcg cggcgtactc cacgcctgac gagaagtacg agaaatacaa
    25561 gttcgatacc attgccgata acgaaaacct gaacatctct tcgaaaggtg gttgggtggc
    25621 aatgctgcaa cagtatttcg cgacggcgtg gatcccgcat aacgacggta ccaacaactt
    25681 ctataccgct aatctgggta acggcatcgc cgctatcggc tataaatctc agccggtact
    25741 ggttcagcct ggtcagactg gcgcgatgaa cagcaccctg tgggttggcc cggaaatcca
    25801 ggacaaaatg gcagctgttg ctccgcacct ggatctgacc gttgattacg gttggttgtg
    25861 gttcatctct cagccgctgt tcaaactgct gaaatggatc catagctttg tgggtaactg
    25921 gggcttctcc attatcatca tcacctttat cgttcgtggc atcatgtacc cgctgaccaa
    25981 agcgcagtac acctccatgg cgaagatgcg tatgctgcag ccgaagattc aggcaatgcg
    26041 tgagcgtctg ggcgatgaca aacagcgtat cagccaggaa atgatggcgc tgtacaaagc
    26101 tgagaaggtt aacccgctgg gcggctgctt cccgctgctg atccagatgc caatcttcct
    26161 ggcgttgtac tacatgctga tgggttccgt tgaactgcgt caggcaccgt ttgcactgtg
    26221 gatccacgac ctgtcggcac aggacccgta ctacatcctg ccgatcctga tgggcgtaac
    26281 gatgttcttc attcagaaga tgtcgccgac cacagtgacc gacccgatgc agcagaagat
    26341 catgaccttt atgccggtca tcttcaccgt gttcttcctg tggttcccgt caggtctggt
    26401 gctgtactat atcgtcagca acctggtaac cattattcag cagcagctga tttaccgtgg
    26461 tctggaaaaa cgtggcctgc atagccgcga gaagaaaaaa tcctgattcg gtgagttttc
    26521 gctaaaataa gggcggtcag ttgaccgcct tttttctttt cgtagggtgg ataagcaccg
    26581 cgcatccgcc acacaaagca acaggaacat catgagcgat aatgacacta tcgtagccca
    26641 ggccacgcct ccgggacgtg gcggcgttgg catcctgcgc atctccggcc taaaagcccg
    26701 tgaagttgcc gaaaccgtgc tgggtaaact gcctaagccg cgctacgccg attatcttcc
    26761 gtttaaagac gccgacggca gcgtgctcga tcaggggatt gcgctatggt tccctggccc
    26821 gaactcgttc accggcgaag atgtgctgga actgcagggt catggcggtc cggtgatcct
    26881 cgacctgctg ttaaaacgca ttctgaccat tcccggcctg cggattgctc gccctggtga
    26941 gttttccgaa cgcgcgtttc ttaacgataa actcgactta gcccaggccg aggcgattgc
    27001 cgatcttatc gacgccagtt cggaacaggc ggcccgttcg gcacttaact cgctgcaagg
    27061 cgcattctcc gcacgggtca accatctggt ggaagccctc acccacttgc gcatttacgt
    27121 cgaagcggca attgatttcc ctgatgaaga gatcgatttc ctctccgacg gcaaaattga
    27181 agcccagctt aatgacgtga ttgccgatct cgatgcagtg cgtgctgaag cacgtcaggg
    27241 tagtttgttg cgcgaaggga tgaaggtggt gattgccgga cgtcctaacg ccggtaaatc
    27301 gagcctgtta aacgcgctgg cgggccgtga agcagcaatc gtaactgata tcgccggaac
    27361 cacacgtgac gtgctgcgtg agcatatcca cattgacgga atgccgctgc atatcatcga
    27421 taccgctggg ctacgtgaag ccagtgacga agtggaacgt attggtatcg agcgcgcgtg
    27481 gcaggaaatt gaacaggccg accgcgtgct gtttatggtc gatggcacca caacagacgc
    27541 cgtggatccg gcagagatct ggccggaatt tatcgcccgt ctgccagcga aactaccgat
    27601 caccgtggtg cgcaataaag ccgatatcac cggcgaaacg ctgggaatga gtgaagtgaa
    27661 cggtcacgcg ttaattcgtc tctcggcaag gacaggtgaa ggcgtggagg tgctgcgtaa
    27721 ccatctcaaa cagagcatgg gctttgacac caacatggaa ggcggcttcc tggcgcgtcg
    27781 tcgccaccta caggcgctgg aacaggcagc ggagcatttg cagcaaggta aagcgcaact
    27841 gttaggtgcc tgggcgggtg aactgctggc ggaagagttg cgcctggcac agcagaactt
    27901 aagcgaaatc accggggaat ttacctctga tgatttgctg gggcggattt tctccagctt
    27961 ctgtattggt aagtaaccgc gcttacgaag ccgtattctg actgtcagat gcggcttcgc
    28021 ttcattgtta gcactcctgt tattcctcaa cccttttttt aaacattaaa attcttacgt
    28081 aatttataat ctttaaaaaa agcatttaat attgctcccc
//
LOCUS       DAKISA010000049        27303 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.049, whole genome shotgun sequence.
ACCESSION   DAKISA010000049 DAKISA010000000
VERSION     DAKISA010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27303
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.049"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..81)
                     /locus_tag="OVW45_002222"
     CDS             complement(<1..81)
                     /locus_tag="OVW45_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 domain-containing protein"
                     /protein_id="HCV5526655.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTL"
     gene            complement(199..1638)
                     /gene="rsmF"
                     /locus_tag="OVW45_002223"
     CDS             complement(199..1638)
                     /gene="rsmF"
                     /locus_tag="OVW45_002223"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HCV5526656.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQISARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(1719..4352)
                     /gene="yebT"
                     /locus_tag="OVW45_002224"
     CDS             complement(1719..4352)
                     /gene="yebT"
                     /locus_tag="OVW45_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HCV5526657.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVNPSVVTL
                     LRENTRIELLNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(4321..5604)
                     /gene="yebS"
                     /locus_tag="OVW45_002225"
     CDS             complement(4321..5604)
                     /gene="yebS"
                     /locus_tag="OVW45_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCV5526658.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            5734..6231
                     /gene="msrC"
                     /locus_tag="OVW45_002226"
     CDS             5734..6231
                     /gene="msrC"
                     /locus_tag="OVW45_002226"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCV5526659.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6328..7026
                     /gene="proQ"
                     /locus_tag="OVW45_002227"
     CDS             6328..7026
                     /gene="proQ"
                     /locus_tag="OVW45_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCV5526660.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7046..9094
                     /gene="prc"
                     /locus_tag="OVW45_002228"
     CDS             7046..9094
                     /gene="prc"
                     /locus_tag="OVW45_002228"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCV5526661.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMRDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9286..10167
                     /gene="htpX"
                     /locus_tag="OVW45_002229"
     CDS             9286..10167
                     /gene="htpX"
                     /locus_tag="OVW45_002229"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCV5526662.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10213..11586)
                     /locus_tag="OVW45_002230"
     CDS             complement(10213..11586)
                     /locus_tag="OVW45_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCV5526663.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFPLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            11763..12554
                     /gene="kdgR"
                     /locus_tag="OVW45_002231"
     CDS             11763..12554
                     /gene="kdgR"
                     /locus_tag="OVW45_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCV5526664.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(12697..12936)
                     /locus_tag="OVW45_002232"
     CDS             complement(12697..12936)
                     /locus_tag="OVW45_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5526665.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            13095..13238
                     /gene="mgrB"
                     /locus_tag="OVW45_002233"
     CDS             13095..13238
                     /gene="mgrB"
                     /locus_tag="OVW45_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCV5526666.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13313..13600
                     /locus_tag="OVW45_002234"
     CDS             13313..13600
                     /locus_tag="OVW45_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCV5526667.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            14270..14413
                     /locus_tag="OVW45_002235"
     CDS             14270..14413
                     /locus_tag="OVW45_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCV5526668.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14426..14635
                     /gene="cspE"
                     /locus_tag="OVW45_002236"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14426..14635
                     /gene="cspE"
                     /locus_tag="OVW45_002236"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCV5526669.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            14801..15610
                     /gene="rlmA"
                     /locus_tag="OVW45_002237"
     CDS             14801..15610
                     /gene="rlmA"
                     /locus_tag="OVW45_002237"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCV5526670.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLSVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFTWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(15607..16173)
                     /gene="mntP"
                     /locus_tag="OVW45_002238"
     CDS             complement(15607..16173)
                     /gene="mntP"
                     /locus_tag="OVW45_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCV5526671.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(16602..17060)
                     /locus_tag="OVW45_002239"
     CDS             complement(16602..17060)
                     /locus_tag="OVW45_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCV5526672.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            complement(17115..17966)
                     /gene="manZ"
                     /locus_tag="OVW45_002240"
     CDS             complement(17115..17966)
                     /gene="manZ"
                     /locus_tag="OVW45_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCV5526673.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(17979..18779)
                     /gene="manY"
                     /locus_tag="OVW45_002241"
     CDS             complement(17979..18779)
                     /gene="manY"
                     /locus_tag="OVW45_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCV5526674.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(18842..19813)
                     /gene="manX"
                     /locus_tag="OVW45_002242"
     CDS             complement(18842..19813)
                     /gene="manX"
                     /locus_tag="OVW45_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCV5526675.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            20276..21832
                     /gene="yoaE"
                     /locus_tag="OVW45_002243"
     CDS             20276..21832
                     /gene="yoaE"
                     /locus_tag="OVW45_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCV5526676.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(21836..23434)
                     /locus_tag="OVW45_002244"
     CDS             complement(21836..23434)
                     /locus_tag="OVW45_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCV5526677.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(23565..24929)
                     /gene="sdaA"
                     /locus_tag="OVW45_002245"
     CDS             complement(23565..24929)
                     /gene="sdaA"
                     /locus_tag="OVW45_002245"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCV5526678.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(25113..25691)
                     /locus_tag="OVW45_002246"
     CDS             complement(25113..25691)
                     /locus_tag="OVW45_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCV5526679.1"
                     /translation="MEYRSLMLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            complement(25695..27056)
                     /gene="pabB"
                     /locus_tag="OVW45_002247"
     CDS             complement(25695..27056)
                     /gene="pabB"
                     /locus_tag="OVW45_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCV5526680.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            27130..>27303
                     /locus_tag="OVW45_002248"
     CDS             27130..>27303
                     /locus_tag="OVW45_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCV5526681.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDED"
BASE COUNT         7040 a         6744 c         6940 g         6579 t
ORIGIN      
        1 taacgttcga tcacccggcg attcaccgtg taattcgccg tcgtcgattt caaaagcgcc
       61 tatgcgcaca ctggttttca tcttcgtgct ctccatttag ctgccggttg tggtacgacc
      121 agtccgatcg caccattttt cagcctagtt gaggcagccg aatgtgccag tcaaaaagtg
      181 cgcttttttt gtgcgctgtc aggcgttacc ggtaaaaagt ttcccatcgc gcaccagttc
      241 acgcggatag ctgtttttca atcgcgaacc aatccgtttg gctaaaccaa tcggttgatg
      301 ctggaaagta accaacacgt catccgccac tggcgcggct tgcgggtaaa catcgcgccc
      361 gcgataccac tcctccgctt cctgcggtgt cagttcaaaa gcgttcacat tgtcgggggt
      421 ggcaagggca ataactgctt catgctgcca gcgataacct ttgttgtgcg tttcggcaag
      481 tttaatcccc aagcgagaaa atcggacttt accgatcagg gcttcaatgc ccaccgggaa
      541 caaccacagt tctttgtcac gctgccagag gcgcaggttt tcatcccagt ttaagccaac
      601 acctgcagcc gcctgacgaa tttgtccagc ttcgcgatct ttcactgggc tgaacggaaa
      661 attaccgact ttgtatttgg gggcgggtaa ggcgggaatc gcctgagttt tacgcagacg
      721 agcaacgaag aagccttcgc agtcgtaaat ttgtgggaaa acatgcaaaa agccttcctc
      781 ggtcagcgcc ttgtttgcac cagggaagag atcaccaagc ggtaaaaact ctactgcgtc
      841 ggggtaagtc tctttcagcc acaggcaaac ggcttcgttt tcttcctggt ttaaggtaca
      901 ggtcgagtaa accagcgtac cgccagggcg taatgcatga aaggcgctgt cgatcagttc
      961 ccgttgggta gctgcgattt cctgattgct ttctggtgac cagtttttta gcgcatcggg
     1021 atctttacgc accacgcctt cgccggagca gggagcgtcc agcaaaatgg catcaaacat
     1081 ttctggcact gccgcaccaa acacgcggcc atcaaaatgt gtgagcgcaa cattactgat
     1141 accacagcgg ctgatattgg catgtaacac ttttacccga ctggcggaaa actcattggc
     1201 aagaattgcc ccttcgttat tcatccgcgc ggaaatttgc gtcgttttgg agccaggcgc
     1261 ggcagcgaca tccatcaccc gctgtggtgc attaccgtca gcaaacaagg cggcgacggg
     1321 caacattgaa ctggcttcct gaatataaaa caggccactt aaatgctcgg cggtactacc
     1381 caatggcaat gcatcttcat tgtcgcgttc aatccagaaa ccttcttcac accacggaat
     1441 tggcgtaagc gtccagccat aaggagcggt taattgcagg aaatcagcaa cggagatttt
     1501 cagcgtatta acgcgaatgc tgcggcgcaa cgggcgctga caggcggcaa gaaaatcatc
     1561 aaaagagagc gtcgaaggca tcgcttcgcg catttgcgtc agaaaggcgt ccgggaaata
     1621 aacggtgtgt tgggccacga gcatatacca ctggcaaaaa aaacaggcgc gcagtttagc
     1681 ataaacgctc cggcgcaggc acgccggagc agtgggcatt atttgggaag cgcagtaccc
     1741 cattcacgcc actctttcgg ttcactttcc tgcaacagga agtgcttgcc ttcctgggct
     1801 ttcggtgcca gtggcgtacc cggaggcgtg gcgaaggcga tgccgccacg gataaactgg
     1861 ttaaaggtgc cggttttcac tacgccgccc gtcagaccaa agtccagact gtaacccgat
     1921 gccaaccaga agacggaatt gttacgcacc aggtgttgat agcgtttact gatgcgcatc
     1981 gcaatcatca cgcgatctga caatgtcccc agcgtcattc cggtaaccgt acctacttcc
     2041 agaccacgga acagcacagg cgtaccaata cctaacgaac cggcttccgg tgcttcgaca
     2101 ataatgctta agccatccag gtaacgcgaa tcagtaatag tagcttcttg caactcaaag
     2161 tcacggcgag gattaccccg acctggttcg acattgatat acggctggag gatagtatct
     2221 agatgctcaa cgcccgcggc tgaaatttgc ggtgtgacca ccgagaagcg cgtaccgccg
     2281 cgggcgaagg tctggacata ctccggatag agcaccgcct ttgcctgtac ttcattgcgc
     2341 gcggtaatca gatccagcgt ctggatttgc ccgatatcaa taccgagata gcgaattggc
     2401 atcccgaccg ccagttttcc ggcatcgaaa gcgtgaagcg taatctgccc accaaccgca
     2461 cgggccgctg tttcggaagc atacagaatt cgtttgtcgc ctttacgctg actggcgctg
     2521 gcaccgctga ggttatcgaa gctaatggct ccctttaatg ctctggagag cggggatgcc
     2581 tgtacggtca ggccactacc attcagctga actttcgccc cgccttctgc ccagaacacg
     2641 ctattgctgg tcagaaggtt gcgatactcc ggcttaatat gcagatcgat atcaaacgcg
     2701 ttagctcgcg gacgcacggt aatcacttca ccaacttcaa atttacggta cagcactacc
     2761 gatcctgcct gcacatccgg cagcgtctct gcactcaaac tcacggttgt ggtgggtaaa
     2821 tcgctaaggc tgttctccag cgctttttcc agattggcat acagtggata gctggctttc
     2881 atctcacctt tatcgcctgg cagaatacgt atcccgccgt taatccattc tgaggcgctg
     2941 gcaccaagaa actcaacgcc atccagcccc accttcacgt cgacacggct gttgacgaca
     3001 aatttgctat cgccttttac cagttcgcga tgctgaggct cgatggcgac ggtaaaggta
     3061 acgcctttgc tggtgagttt acgatcaata acctggccta cctgcacgcc gtgaagaatg
     3121 agcggctgac cagcatcaat accgtaactt tccggcgcgg tcagggtcag cgtcagaaca
     3181 tcaggttcat gcagcagtgc tttttcgcct ggcacaacaa cgaactcttt gcgtggctcg
     3241 ccatcgccgg gtaccaactc gaaggttttg ccagtcagca gggcgctgag attggcatcg
     3301 ctaagggata atttcggatt aagtaattcg atgcgggtat tttcacgaag cagggtaacg
     3361 acgctgggat taactgtcat ttccccagtg actttaccac caggatttaa atccagttta
     3421 gttagctgtc cgacttccag tccctgatac attaatggcg tcgagtcggc ggttaatccg
     3481 gccccactcg gcagttccag tttgattatt acgccacgct ggctatgggc gaggtcttca
     3541 tacagaccaa aggtatcttc cgcctcggca ggtttcgact cttcaggtga atcgaaggca
     3601 atcgcaccgt taaccagtgc cgccagactt tccagtttca ccttcgcgcc actgatactg
     3661 acgttggcat caacgccgga aacgttccag aaacggctac cttttttcac caggtcggta
     3721 aaacgccgct cgatcaggac atcaatcacc acgccttgct tgttgggatt gatggcatag
     3781 tcgtagactt ttcccacagg gatcttgcgg aaatagacca atgaaccgct gttcagcgaa
     3841 ccgagatcgg gggcttgcag gtggatcatc agatcgccat tgtccagtcg atatttcggt
     3901 tgggtatcga gtgcgacaaa gtgatcctgc tctttacctt tacctggcat catgccgata
     3961 tagttaccac cgacgagggc gtccagcccg gagacacctg ccaacgatgc ttttggcgtc
     4021 accagccaga actgagtctc ttcgcgcagc gcatctttca tatcggactt gatgctgacc
     4081 ttgacttcaa tcttacgaag atcgtcgctg aggctgatat cctgcactgt tccgacttca
     4141 acgccctgat aacgaacagg cgtacggccc gggacaatac catccgccga cataaagtcg
     4201 atggtgacgg tattaccccg gtcctgataa ctgtcccaaa tcagccaacc ggcaatcatt
     4261 agcgcgatga aaggcagcag ccagaaaggt gagatacggc gtttattttt aatctgcgct
     4321 tcagtcgtcg aagcgggcgt ttcctgactc atgtgcatcc caaagtaagc ggctgtccag
     4381 ccattccaca gcaagaatag tcaaaattac ggctgcgcca aaataaaacg cagccggtcc
     4441 catagtaaaa gcgaggatct gatcgcgatt aatcagcgac atggttaaag atatgacaaa
     4501 caggtcgagc atcgaccagc gtccaatcca ggtcaccatc cgcagtaaca gaatgcgtgt
     4561 gcgtaaacct tgctggcatt taaaatgaat gctcaacagt aaagtgaaca tgacgatcac
     4621 tttagtaaac ggtaccagaa tactggcgat aaacacgatt ccggcgaccg caatgttgct
     4681 acttgccagc gacataatcc cggaaagaat cgtatcttcc tgccggccac cattcagata
     4741 aatgatagaa ataggcaata ggttggctgg taacaacaaa acgattgacg ctaacagcgc
     4801 cgcccagcat ttttgcaaac tatgacgacg acgcaggcgt agcgggatat ggcaacgcgg
     4861 gcagcgacca cgctgatcag gatagccggt aaaatggcac ccaagacaga cacgaagttt
     4921 ctcgtcccta cgcgtagcgg ggcgctgcgg ataatatcgc tcccacagct cctcaacatt
     4981 aagatgtgac aacgtcaccg tcgttaaaat caccaacgcc acaaaagaga acaagccgac
     5041 acccgcctgg atatgggcat aatcctgtac ctttatagaa gcaacgccaa tgccgaccag
     5101 gtagatatcc agcattaccc actctttaag acgctcaagc atcagcagta ccggacgtaa
     5161 attcattccc agtcggttac caaaccataa atatgctatg gaggacacca gaatgagggg
     5221 ggcaccaata acgcagaaaa agaccatcgc ccccgttatc gtatcgccct gtttggtcat
     5281 ttgccagatg ccttgcatga cgttggcgtc gatacgaata cctaacagcc agatatgcaa
     5341 cagcggttcg ccccaggcaa acggcatcag caacagcatg gtgaaagcca ttgccgccag
     5401 gcgcgttagc gaccagtcgc gcccgtcgcg aatttttgcc tgacagcgcg gacaataggc
     5461 actctgatga gaatttatct cgggcaggct aaacagcatg tcacattgcg ggcaacgttg
     5521 gtaatcacca cgcggcagtt cctcgccgat tgccctcact gttatctttt ttgtcggcgt
     5581 aatttgtggt gtgttaagag ccatgacgtg ccaaatgtaa taagaattaa cattaatctt
     5641 aacccatgat gggaatgatc ttgagcatta acgcatttaa tgcttatttt aataggctgg
     5701 cagttccagg taagacaaac aagtgattat ataatgaaca aaacagaatt ttacgcggat
     5761 ttaaatcgcg actttaacgc gctgatggcg ggagaaacca gttttctggc aacgcttgcg
     5821 aacaccagtg cgttgttata cgagcgtctc actgacgtaa actgggcagg tttttatttg
     5881 cttgaggacg atacactggt actcggacca tttcagggca aaattgcctg tgtccggata
     5941 cccgtcgggc gcggggtgtg cggcactgcg gttgcccgca atcaagtgca gcgtatcgag
     6001 gatgttcacg cgtttgacgg gcatattgcc tgtgatgcgg cgagtaattc tgaaattgtt
     6061 ctgccgctgg tggtgaaaaa tcagattatt ggtgttcttg acatcgatag taccgtcttc
     6121 ggtcgcttta cagacgagga cgagcaaggc ttacgtcagc ttgtggcaca gcttgaaaaa
     6181 gtgcttgcaa cgacggatta caaaaaattc tttgcgagcg tcgcaggata atcaacggat
     6241 aacgtagcat ttactggtag cgtcattata atgacgcctg ttcatgcctg cgcttgttgg
     6301 ctacgtccgt tgtaatcagg aaatttcatg gaaaatcaac ctaagttgaa tagcagtaaa
     6361 gaagtaatcg cgtttctggc cgaacgtttt ccccactgtt tcagtgcgga aggtgaagcg
     6421 cgtccgctga aaatcggtat ttttcaggat ttggtcgatc gtgttgccgg ggaaatgaac
     6481 ctgagcaaaa cgcaattacg atccgcttta cgtctctaca cttcgagctg gcgttatctt
     6541 tacggtgtta aacccggcgc aacgcgtgtc gatcttgacg gcaacccatg cggtgagctg
     6601 gacgagcaac atgtagagca tgctcgcaag cagcttgaag aagcgaaagc gcgtgttcag
     6661 gcacagcgtg ctgaacagca agcgaaaaaa cgcgaagctg ccgcagctgc tggtgagaaa
     6721 gaagacgcac cgcgccgcga acgcaagcca cgtccgacca cgccacgccg caaagaaggc
     6781 gctgaacgta aacctcgtgc gcaaaagccg gtagagaaag cgccaaaaac agtaaaagca
     6841 cctcgcgaag aacagcacac cccggtttct gacatttcag ctctgactgt cggacaagcc
     6901 ctgaaggtga aagcgggtca aaacgcgatg gatgccaccg tattagaaat caccaaagat
     6961 ggcgtccgcg tccagctgaa ttcgggtatg tctttgattg tgcgcgcaga acacctggtg
     7021 ttctgaaacg gaggccgggc caggcatgaa catgtttttt aggcttaccg cgttagctgg
     7081 cctgcttgca atagcaggcc agaccttcgc tgtagaagat atcacgcgtg ctgatcaaat
     7141 tccggtatta aaggaagaga cgcagcatgc gacggtaagt gagcgcgtaa cgtcgcgctt
     7201 cacccgttct cattatcgcc agttcgacct cgatcaggca ttttcggcca aaatctttga
     7261 ccgctacctg aatctgctcg attacagcca caacgtgctg ctggcaagcg atgttgaaca
     7321 gttcgcgaaa aagaaaaccg agttaggcga tgaactgcgt tcaggcaaac tcgacgtttt
     7381 ctacgatctc tacaatctgg cgcaaaagcg ccgttttgag cgttaccagt acgctttgtc
     7441 ggtactggaa aagccgatgg atttcaccgg caacgacact tataaccttg accgcagcaa
     7501 agcgccctgg ccgaaaaacg aggctgagtt gaacgcgctg tgggacagta aagtcaaatt
     7561 cgacgagtta agcctgaagc tggcaggaaa aacggataaa gaaattcgtg aaaccctgac
     7621 acgccgctac aaatttgcca ttcgtcgtct ggcgcaaacc aacagcgaag atgttttctc
     7681 gctggcaatg acggcgtttg cgcgtgaaat cgacccgcat accaactatc tttccccgcg
     7741 taataccgaa cagttcaaca ctgaaatgag tttgtcgctg gaaggtattg gcgcagtgct
     7801 gcaaatggat gatgactaca ccgttatcaa ttcgatggtg gcaggtggtc cggcagcgaa
     7861 gagtaaagct atcagcgttg gtgacaaaat tgtcggtgtt ggtcaaacag gcaagccgat
     7921 ggttgacgtg attggctggc gtcttgatga tgtggttgcc ttaattaaag ggccgaaggg
     7981 cagtaaagtt cgtctggaaa ttttacctgc tggtaaaggg accaagaccc gcactgtaac
     8041 attgacccgt gaacgtattc gtctcgaaga ccgcgcggtt aaaatgtcgg tgaagaccgt
     8101 cggtaaagag aaagtcggcg tgctggatat tccgggcttc tatgtgggtt tgacagacga
     8161 tgtcaaagtt caactgcaga aactggaaaa acagaatgtc agcagtgtga tcatcgacct
     8221 gcgtagcaat ggcggtgggg cgttgaccga agcggtatcg ctctcaggtc tgtttattcc
     8281 tgcgggtccc attgttcagg tccgcgataa caacggcaag gttcgtgaag acagcgatac
     8341 cgacgggcag gtgttctata aaggcccgct ggtggtactg gttgaccgtt tcagtgcttc
     8401 ggcttcagaa atctttgccg cggcaatgca ggattacggt cgtgcgctgg ttgtgggtga
     8461 accgacgttt ggtaaaggca ccgttcagca ataccgttca ttgaaccgta tttacgatca
     8521 gatgttacgt cctgaatggc cagcgctggg ttctgtgcag tacacgatcc agaaattcta
     8581 tcgcgttaac ggcggcagta cgcaacgtaa aggcgtaacg ccagacatca tcatgccgac
     8641 gggtaatgaa gaaacggaaa cgggtgagaa attcgaagat aacgcgctgc cgtgggatag
     8701 cattgatgcc gcgacttatg tgaaatcagg cgatttaacg gcctttgggc cggagctgct
     8761 gaaggaacat aatgcgcgta tcgcgaaaga tcctgagttc cagaacatca tgaaggatat
     8821 cgcgcgcttc aacgctatga gggacaagcg caatatcgtt tctctgaact acgctgtgcg
     8881 tgagaaagag aataatgaag atgatgcgac gcgtctggcg cgtttgaacg aacgctttaa
     8941 acgcgaaggt aaaccggagt tgaagaagct ggatgatcta ccgaaagatt accaggagcc
     9001 ggatccttat ctggatgaga cggtgaatat cgcactcgat ctggcgaagc ttgaaaaagc
     9061 cagacccgcg gaacaacccg ctcccgtcaa gtaatatcaa tcaggcacaa gaaattgtgc
     9121 ctgatttttt aacagcggca agatgccgta aatcagatgc tacaaaatgt aaagttgtgt
     9181 ctttctggtg acttacgcac tatccagact tgaaaatagt cgcgtaaccc atacgatgtg
     9241 ggtatcgcat attgcgtttt gttaaactga ggtaaaaaga aaattatgat gcgaatcgcg
     9301 ctcttcctgc taacgaacct ggccgtaatg gtcgttttcg ggctggtact gagcctgaca
     9361 gggatacagt cgagcagcgt tcaggggctg atgatcatgg ccttgctgtt cggttttggt
     9421 ggttccttcg tttcgcttct gatgtccaaa tggatggcat tacgatctgt tggcggggaa
     9481 gtgatcgagc aaccgcgtaa cgaaagggaa cgttggctgg tcaatactgt agcaacccag
     9541 gctcgtcagg cagggatcgc tatgccgcaa gtggctatct accatgcgcc ggacatcaac
     9601 gcttttgcaa ccggtgcgcg ccgtgatgcc tctctggttg ctgtcagcac cggtttgctg
     9661 cagaacatga gcccggatga agccgaggcg gtaattgctc acgaaatcag ccacatcgcc
     9721 aatggtgata tggtcaccat gacgctgatt cagggcgtgg tgaacacctt cgttatcttt
     9781 atttcccgta ttctggcgca gcttgccgcg ggttttatgg gcggaaatcg tgatgaaggt
     9841 gaagagagca acggcaaccc gctgatctac tttgcggttg caacggttct ggaactggtg
     9901 tttggtattc tggcgagcat tatcaccatg tggttctcgc gtcatcgtga attccatgct
     9961 gatgccggtt cggcaaaact ggttggtcgc gagaaaatga ttgccgcgct gcagcgcctg
    10021 aaaaccagct atgaaccgca agaagcaacc agcatgatgg ctttctgcat taacggtaag
    10081 tcgaaatcgc tcagtgagtt gttcatgact cacccgccgc tggataaacg aattgaagct
    10141 ctgcgtacgg gtgaatacct gaagtaatat gaagtaaata ctaaaaagcg cgtcctgatc
    10201 gacgcgcttt ttttatgccc tggatcgtgg ctgagtgata cgtaaaccac tgacacaggc
    10261 agcaatcact gccagaatag ccgcagccat cagcgaaacg tgcgtaccat tatcaccgaa
    10321 ctgatttagc atcagcgcca ccagtgccgc gccgctactc tgacccagta gacgagccgt
    10381 tcctaacatg ccactggctc caccgctacg ttcgcgagga gcggaggtaa taatggtgtg
    10441 gttattgggt gactggaaca agccaaatcc agcgccacat aagatcatcg gccagataat
    10501 attgatatcc gcaggtgacg cgggcagcag aaccagggga aaaagccccg cagccatgat
    10561 gaacaaccct agagcaccca gcaatcctgc atgtacgcgt tcaatcaaat agcctgccaa
    10621 cggagccatc accatcgttg ctaacggcca cggtgtcaga agtaaacctg tttcgacttc
    10681 actacgcccg agcacggttt gcaggtaaaa gggcagggaa accattgcca gcatttgtgc
    10741 gcagaaagag caaacagatg tgcaaataga aagtgaaaac agcgggatac gcagtaaatc
    10801 caccggtagc agcggtacgg gaagagaaag ctggcggcga ataaagaaaa taccaacaac
    10861 aaccattacc accagttccg cagcaattaa cgtcagcgat tgcccctgag cgaaaccact
    10921 caacgcagtg ataagcaggc cgaaggttaa cgcgttcatc acggcgctgg gcaggtcgaa
    10981 acggggttta ctggcgcgag aaccattggg tggcagaaaa cgcatcgcca gaagcagggc
    11041 gataatacct aacggtacgt tgattaaaaa taaccatttc caggatgcga tggagaggat
    11101 tgctgcagca attgtcggcc cggcagcaga agagacggca acaataaacg agtttatgcc
    11161 catccctctc cccagaaaac gttgtggata gatcaggcgg ataagtgcgg tattaacgct
    11221 catcaacgct gcaccgccga aaccttgtat gacacgcgca agggtgagca tttgcagcga
    11281 atcagaaagg gcgcagaaca gtgaagacaa cagaaaaacg accagaccgc atttataaat
    11341 acgtcgatag ccaaacatat cgcccagaaa cgaaaacgag agcagggaga tgacaatggc
    11401 gatttgatag gcgttcacta cccagatgga actggctggc gtggcatgaa ggtccgtggc
    11461 gattgttggc agggcgacgt tggcgattgc gccgtcaagg acagccatcg aaatgccaat
    11521 cacaatggtt aatatcgcac cgtatcgctg gggcaatggc aggccgtcgg cctgaacttt
    11581 tggcatagga attttatatc tttggtgaat aatcaacagg ggtatgattc tagcactgat
    11641 attcacggtg gatgtcgcag aattgtaact aaccggcaaa agatggattg cgaccaccct
    11701 ggtgcgaaat tataataaaa aacagttctg atttttataa aacactcgca atgaggtgat
    11761 aaatggctaa cgcagatctg gataaacagc ctgattctgt atcttccgtg ctaaaagttt
    11821 ttggcatttt gcaggcgctg ggtgaagagc gcgaaatagg gataaccgag ctgtcgcagc
    11881 gcgtcatgat gtcaaaaagc accgtttatc gctttttaca gaccatgaaa accttaggtt
    11941 atgtggcgca ggaaggggag tcggagaaat attccctgac cctgaaattg tttgaactgg
    12001 gcgctcgcgc gttacaaaac gtcgatttaa ttcgtagcgc agatatccag atgcgtgaga
    12061 tctcccgcct gaccaaagaa actatccacc tcggcgcact ggacgaagac agtattgttt
    12121 acattcacaa aattgactct atgtacaatt tgcgcatgta ttcacggatt gggcgtcgta
    12181 atccgctgta cagcaccgcg attggtaagg tactgctggc atggcgcgat ctcgatgaag
    12241 tgaagcaaat tcttgagggc gtggagtata aacgcagtac cgagcggacc atcaccagta
    12301 cagaagcgtt attacccgtt ctggaccagg tgcgcgagca ggggtatggc gaagataacg
    12361 aagagcagga agaagggctg cgatgcattg cggtaccggt atttgatcgc tttggcgtgg
    12421 tcattgccgg tttgagcatc tccttcccga cgttgcgttt ctctgaagag cgtttacagg
    12481 aatatgtcgc aatgttgcat accgcagcgc gcaaaatttc tgcccaaatg ggttatcacg
    12541 actatccgtt ctgatgagag taagaacctg tcggaatatc aaacagacag gttctttatt
    12601 tagcatgaga aaaataaagt tgaaggtgcg ttatattaaa cgcgcttgct ataagaatat
    12661 tttactcatg agtgagaatc tggttgttta ttacccttaa ccattatcga ccacgatatt
    12721 gcttttacgt aacagtgggc aatctgttat ccccaagaaa ccacttttag tgtgcaagta
    12781 ttgtaccgtg ctggtgcctc tggcagtcag ataggtacat tgcaaaccta atcctgcggc
    12841 attctctttg cttccaatca aaacgccata tccgctgagt aataatccta tccataccag
    12901 tgctatcagc ataactgtgc gaatgatgaa tcgcattaca acctcttctc tttttatgtt
    12961 cgcttaatcg tagcggcaat atgcgctgaa gcaagctact cattccgaaa aagcacgaat
    13021 atcgacatag ttaggcgctg tttaactaac gcatgctagt ttaatgacat aaggtaggtg
    13081 aagcggagat tggagtgaaa aagtttcgat gggtcgttct ggttgtcgtg gtgttggctt
    13141 gcttgctgct ttgggcgcag gtattcaaca tgatgtgcga tcaggatgta caatttttca
    13201 gcggaatttg tgccattaac cagtttatcc cgtggtgata tcattttttc agcactagat
    13261 tttcttcctc tccagcagtg gtagaatagc gcctctactc agaggtggtg aaatgaacga
    13321 agttgtaaat tcaggcgtga tgaacattgc gtctttggtt gtatcggtgg tggttcttct
    13381 tatcgggctc atcttgtggt tttttatcaa tcgtgccagt tcacggacta acgaacagat
    13441 tgaactgctt gaggcgttgc tggatcagca aaaacgtcaa aatgcactgt tacgtcgttt
    13501 gtgcgaagca aacgaaccag agaaagcaga taaaaagacc attgagagtc aaaaatcggt
    13561 tgatgacgaa gatattattc gcctggtcgc cgaacgataa tccttcctca atcttgagta
    13621 accccgtcgt tgaagggtta ctcaattcat cctgattata cgcactgtca tggtgcaact
    13681 gcttcaaatt aaaactctgg tctgacttca aataattatc atattattta tttgcgtaac
    13741 gggatatcag aaaaagatat tttcctcatt aatttattag gatgtttaca tcggatttgt
    13801 gattaagcgt ggtattattt attagcgaaa cgtttctctc ttgatttttt tgctcattca
    13861 tcaatttttc ttattttaaa ttttcaatcc ctttggggat tgacttctct ttagggtaat
    13921 tgatagccgt taaccgactg ttttatgaga aaaagtgata taacttttta ttcattgcat
    13981 agcaaaaaat gtgatattgc acgcactatg taataacttc tcccactggc ctggaacaac
    14041 tgaacttatt gaactatgtt agaaaatacg ccagtttaag tatctgcctg aactggcaag
    14101 gttaagcaca atgatatatc ggcgcgtatt ccgttgcata agtgtgcaaa aaaagtggaa
    14161 gacgtatcga gatttgtgcg tctgatcgag acatgtttaa aaatggcttg ccataattaa
    14221 cgttgtatgt gataacagat ttcgggttaa acgaggtaca gttctgttta tgtgtggcat
    14281 tttcagtaaa gaagtcctga gtaaacacgt tgacgttgaa taccgcttct ctgccgagcc
    14341 ttatattggt gcctcatgca gtaatgtgtc agttttatct atgttatgcc tgcgggcgaa
    14401 gaaaacaatc taaggaattt ttcaaatggc aaagattaaa ggtcaggtta agtggttcaa
    14461 cgagtctaaa ggttttggct tcattactcc ggctgatggc agcaaagacg tgttcgtaca
    14521 cttctccgct atccagggta atggcttcaa aactctggct gaaggtcaga acgttgagtt
    14581 cgaaattcag gacggccaga aaggtccggc agctgttaac gtaacagcta tctgatcgaa
    14641 tccactgatc tgaagcgtga atacgcttca atctcgctat aaagcctcgt cgaatgcgag
    14701 gctttttact atgctttatc ttcgctcctg gcgttcggat atttgcccgc cgcgtgattc
    14761 gcgttacact tgcggccttt agtatcctgc cggagttgtc atgtcttttt cctgtccact
    14821 ttgccatcag cctctttcgc gtgaaaaaaa cagctatatc tgtccccagc gacatcagtt
    14881 tgatatggcg aaagaagggt atgtcaatct gctgtccgtt cagcataaac ggtctcgtga
    14941 tccgggcgac agcgcggaaa tgatgcaagc acgccgcgca ttcttagatg ccggacatta
    15001 tcagccgctg cgtgatgcaa ttgtcgccca actgagggaa cggcttgatg ataaggccac
    15061 ggcggtgctg gatattggct gtggtgaagg gtattacaca cacgcatttg ccgatgcgtt
    15121 gcccgaaatc accacgtttg gtctggatgt ttcgaaggta gcgataaaag cggcggcgaa
    15181 acgctatccg caggtcactt tttgtgtcgc ttccagccac cgtttgccgt tttccgatac
    15241 cagtatggac gccataatac gtatttacgc gccgtgtaaa gcagaagaat tagcacgagt
    15301 agtgaagccc ggcggctggg tcattactgc cacgccggga ccgcgacatt tgatggagct
    15361 gaaggggctg atttacaatg aagtacatct tcatgcacct catgcagaac aactggaagg
    15421 ttttacatta cagcagagtg cggagttgtg ttatccgatg cgtcttcgcg gtgatgaagc
    15481 cgtcgcatta ttgcagatga cgccgtttac ctggcgtgcg aagccagaag tctggcaaac
    15541 actggcagca aaagaagtgt tcgactgcca gacggacttt aatattcacc tctggcagcg
    15601 ttcttattaa ccgtggaagt gcgtccagag gatctggacg ccgatgccga tcagtaccag
    15661 cccgccgaga atttccgctt ttttcccaat aattgagccg ataaagcgac caaccatcat
    15721 ccctaatgtt gacataatca aggttgcaca accaatggcc aatgcggtcg cgataatgtt
    15781 gacctgcagg aaagcaagac caacacccac agccatggca tccaggctgg tggcaatcgc
    15841 ggtggttacc agtagccaga aaccgtgtcg acggcgcggc tcttcatctt catcatctgc
    15901 gccacgaaaa ccctcaataa tcattcgccc gccgaggaat atcagcagca caaacgcaat
    15961 ccagtggttc cattcaagga caaaccggct ggctaacatg cccattcccc agccgatcag
    16021 cggcgtcagg gtttcgacgg caccaaaaat aaggccggtt cgcaatgctt cagaaaattt
    16081 cggtttatgg agggtggcac ctttaccgat tgatgcagca aatgcatcca tcgacatacc
    16141 aaacgcaaga agaacagtag cagtgatatt catgacaatg tcctgaccgg ggtatccata
    16201 aaacacatca tcgcccccag caaacatgca tacatgaaca acaagccgtg atggttttcg
    16261 cgttcatgca ggcatagcga ttgatgtgca tatggtctcg cctgactgaa agtattcagt
    16321 ccgtacgtgc cacgttttgc aacgagtatg ttgacacgta catttccgga atctggaaat
    16381 cggctactcc ccaaatgagg gcgcaacctt aacatatttt gagaatataa aacaactgtt
    16441 ggaatatatt atttcattgc tttattgata acgattttca tttggattta tatatcaatg
    16501 aaacgttaaa ataaataaca aataagggaa gaggtaatat agcctaagct atgtctgatt
    16561 attaaaaata cactaaacat agctttggct aaattcataa cttattgagt tgaaacgaga
    16621 agcttataaa ttttttccag atcgtcgata tttcgaacgc ggattaacag ccgacgctgt
    16681 tctaattgca tcaccaacac gccatcttcc gacaagttca tcgctttgat tcggctatat
    16741 tcaatccaga cattggcgaa gaaaaaacct ttttgtttaa agatgatctt cggaacgcgg
    16801 atccagaata tataaaaacc catcagagcc agtgcgctta ataaccatgt cgttattaac
    16861 gcaccatgat tcgtgacgtt gttatagata agaatgacaa tcaatccgac gaagataacg
    16921 ctatcgatgc gaccacgccg gagcaaagga attgccagca gggtggggcc gttacggcgg
    16981 ggcatgatga actgatcgta gatcgcgaag gccaggagtg cggcgatgaa aagaatcagt
    17041 accaggtccg tgattgtcat taatcctcca gataaaaaaa cggggccaaa aggccccggt
    17101 agtgtacaac agtcttacag tcccagcagg ccgcaagcgt aaccagcgat accgatgacg
    17161 aagaagccaa cgatgatcca cagcgggtta acttttttgc gcagtagcca catacaagca
    17221 aaggtcagca gcagtggtac caggcctggc attaactggt ccagaatagt ctggacagta
    17281 gtaacgtgtt ctttgcccgt ctggtcagta atgcgagaga caaccagcgg gatgttgaca
    17341 tgtgtccact tgttaaccaa tgcccccatg acaaacaggc cgaggataga cgccccttcc
    17401 gtcagttttt gcaggaagcc accacccata tctttaacga tatcgatacc tttggagtaa
    17461 ccatacgcta cgccgtagta acgggttgcc agacgcacca ggttaaacag gatgaagaac
    17521 agcagcggac ctaacaggct gccgctcatc gcgataccgg cacccagtgc tgcaaatacc
    17581 ggacgtacgg ttccccagaa gatcgggtcg cctacaccag ccagcggccc catcaaaccg
    17641 actttgatac cgttgatagc accgtcgtcg atctctgcgc cattagcacg ctgttcttcc
    17701 agcgccaggg ttacgccgag aatcggcgca gccacgaacg gctgggtgtt aaagaactcc
    17761 aggtgacggc gaatagcttg tttacgagct tcgttgttct cagggtagag gcgacgaatt
    17821 gccggtacca tagagaagca gaaacccagt gcctgcatac gttcgaagtt ccatgaaccc
    17881 tggaagaggt tagaacgcag gaagacgcca cgaatatcac tttgagtgag ttttttctcg
    17941 gtggtagttt gagttgtatc aaccatttcg ctcacctgtt agtccagttc gttatcgaga
    18001 tcgttgttac cagctgcctg agcaggcgca ccggctacgc ggttgtattt cgggctaagt
    18061 tggatgtaga gcactgccat aacagtacca atcacaccca gagcaaccag gttaaagttg
    18121 gtgaatgctg cggttacgaa gccgaggtag aagaacggca tcaggtagcc agcacgcatc
    18181 atgttgataa ccatcgcata accaaccacc acgatcatgc caccggcgat attcagacca
    18241 ttggtcacca cttccggaat cgcattcagc atgttctgta cttcgctggt accaacagac
    18301 agcgcaacga tgacggccgg aatagccaca cgcattgctt gcaggaacag agaagaaacg
    18361 tggatccagg aaatcgctgt caggttgccg ttatcagcag ccttatccgc agcgtgctgg
    18421 aaagcaacgg taatagtacg aacgatgatg gtcagtacct ggcccgcagc ggccagaggg
    18481 attgccagtg cgatacctgc accaatgctc tgatgacctg cgataaccag aatggtagaa
    18541 atgatagaag ccagagcggc gtcaggcgca actgcagcac cgatgttcat ccagcccagc
    18601 gcgatcattt ccagcgtacc accgataata ataccggttt tcatatcccc aagaacgata
    18661 cccaccaggg tacacgcgat tagcggacgg tgaaactgaa attcatcgag gattgatccc
    18721 atacctgcga tacaggctac gataaatacc agcacaattt gaagagtggt aatctccatt
    18781 gtacttctcc tgtttacgta agacttaagt gtgaaaactg agtgataatc aacacaatac
    18841 gttacttatc gattttgctg atcagatcca tcattttcag tttcggatcg gtggaaacct
    18901 tacggacttc cagctcaata ccgcgcgcat tcagtttctt gaacgcctcg atatcttttt
    18961 catcaaccga aaccgcgtta ttcacctggg ttttaccctg acggaatgcc ataccaccga
    19021 cgttaacaga ggtgattttc acgccgcctt caacgagacg ctctacatct gttgggttgg
    19081 taaataacag cattacgcgt tcgccagcat atttcgggtt gttgtagacg cgaatcattt
    19141 tggcaacatc aactacgtgt gctgttacgc ccggaggtgc aacctgggtg agcagtgtct
    19201 tacgaacggt atccgcagcc acttcatcac taacaacaat aatacgggag acattggttt
    19261 ctttggtcca gcgggtggcg acctgaccgt gaatcagacg gtcgtcgata cgcgcaaggc
    19321 caataaccat gtagtcgttt ggccccattg gttttgccgg agttggagcc gcttttggtg
    19381 ctgcggcagc gggtgctggc gcggcttttt caaccggttt ggctttcagt gctttcacgc
    19441 cttcacggcc tgtttctact gccagcgcaa ccagttcatc aaagcttggg tcatcatcac
    19501 gagccattaa cgtttccacg agcattggaa tgttaacgcc tgcaatgact tcataatgct
    19561 ctttgtcgac gacaatgcgg ctggcagcat tgaacgggct gcctccccat gtatcaacga
    19621 gaaacagcac gcctttagtg gtgtcgagtt ttgccaactg agcgttgtac ttttcaatca
    19681 gcgtttcggc attttcacct ggaacgaaat cgatccagcc gacgttttcc tgctcgccta
    19741 acagcatttc tgccgtttta agcaactgct ctgcagccca accatgtgtg cctataacaa
    19801 tagcaatggt cacttgctac ctcctttatt atcgttaaca cctcaacgtg ccagatgtat
    19861 ttttgaatcg tcgtccacaa tcgaatcgat tcagataagg gctacaacca aaaacccctg
    19921 cgtttcgcga tttattttag atatcgaaaa aattatttta tgtgatgaag atccgtaatt
    19981 taactttcga ttagcagaaa tttcgaaagg taaaatatcc ttgtcacatt cgtttgcaaa
    20041 ggaaggtaaa tctttgccaa atcagaggcg tctctgatat gtttaactcc cgtttaacaa
    20101 ccatggagta tagggcagta gcccgcagta tggatcgtca ccgacgtcat ttcagcatca
    20161 ggccttttaa cgcctgcctt tctggcactc tatgccgcac ctttcgtttg cattttgtcg
    20221 ttacgcctgc attatttctg gcgtcgaata gctattcctt aagcaggagc ttgtcatgga
    20281 attcttaatg gacccctcaa tttgggcggg gctactcacg cttgttgttc tcgaaattgt
    20341 gctgggtatc gataacctgg tcttcatcgc cattcttgct gacaaactgc cgccaaaaca
    20401 acgcgataaa gcgcgtttgc tggggttatc actggcgctg attatgcgtc tggggctgct
    20461 gtcgctgatt tcatggatgg tcacgctgac caaaccgcta tttaccgtca tggatttctc
    20521 cttctccgga cgcgacctga ttatgttgtt cggggggata ttcttgctgt tcaaagcaac
    20581 aaccgaactg catgaacggc tggaaaaccg cgatcatgat tccggccacg gtaaaggcta
    20641 cgccagtttc tgggtggtcg tcacacagat tgtcatcctt gacgccgtct tctcgttgga
    20701 tgcggtaatt actgcagtag ggatggttaa ccatctgccg gtgatgatgg cggcggtagt
    20761 gattgcgatg gcggttatgt tgctggcatc caaaccgctg acgcgattcg ttaaccagca
    20821 ccccacggtg gtggtgctct gtctgagctt cctgttaatg attggtctga gtctggtggc
    20881 agaaggtttc ggtttccaca ttccgaaagg ttacctgtat gccgcgattg gcttctcgat
    20941 catcatcgaa gtgtttaacc agattgcgcg tcgcaacttt attcgccacc agtcgacttt
    21001 gccgctgcga gcgcgtactg ccgatgccat cctgcgtttg atgggcggga aacgtcaggc
    21061 caatgttcag cacgatgccg ataacccgat gccgatgccg atcccggaag gtgcatttgc
    21121 cgaagaagaa cgttacatga ttaacggcgt actgacgctt gcgtcgcgtt ctctgcgcgg
    21181 gatcatgacg ccgcgcggtg aaataagctg ggttgacgct aatctcgggg tcgatgaaat
    21241 ccgcgagcaa ctgctctctt caccgcacag tctgttcccg gtatgtcgcg gtgaactgga
    21301 tgaaatcatc ggtattgtac gtgctaaaga actgctggtg gcgctggaag agggcgttga
    21361 tgtggcggcg attgcttcgg cgtctccggc gattatcgtc ccggaaaccc tcgatccgat
    21421 caacctgttg ggcgtgctgc gtcgtgctcg cgggagcttt gttatcgtga ccaacgagtt
    21481 tggtgtggta caaggtctgg tcacgccgct ggatgtgctg gaagccattg cgggtgaatt
    21541 cccggacgct gacgaaacgc cggaaatcat tactgatggt gacggctggc tggtaaaagg
    21601 cggtacagat ttgcatgcct tgcagcaggc gcttgatgtt gagcaccttg ccgatgacga
    21661 tgatatcgcg acggtcgcgg gcctcgtgat ctcggcaaat ggtcacattc cccgtgtggg
    21721 cgatgtgatt gatgtagggc cactgcatat caccatcatt gaagccaatg attatcgtgt
    21781 tgatctggtt cgcattgtta aagagcaacc ggcgcacgat gaagatgagt aagcattaac
    21841 gtaacggcat aatgggcgtg atatgtccat tatgccgggc gggcggcggt tggctgcccg
    21901 ccagccattt gggaaaatca cgtagcggca tcggctgtgc gtacaaatac ccttgcaaaa
    21961 tatgcacgcc atgacggcgc aaatacttcg cctgttcttg cgtttccacg ccctccgcca
    22021 ccagttcaat attcagtctt tgccccagcg cgatgatgat atcggtcacc gtcgagttaa
    22081 ccgcgtcaga acctatagct gcggtaaatg acttatcaat tttcagcacg tcaggacgta
    22141 atgtttcaag ccaggaaaat gaactgttgc cggtgccgaa gtcatcaatc gccagtttga
    22201 cgtttttacg atgtagctcg cgggcaatcc gataatcaac atccagtaag gcatcgcgtt
    22261 cggtgatttc gaggatcagt tgctgaatcg ggtgagcgct aaaccagtac tgattgagat
    22321 cttttatcag cacaccacgg cgaaaatggc tgggtgcgac gttaatgcca acatgaaact
    22381 gactactcat cgggaaaaca tggcgctgac gaatggtttc tgccatcaca tagcgggtca
    22441 gtggcacaat taaatgatgt tcttccgcga taggaataaa cacatccggt gaaatccagc
    22501 cctgacgcgg attgttccag cgcagcaaaa tctctacacc aatacattgc tggctgcgcg
    22561 cattaagcaa aggctgacag aacaattcga actcatgttg cgccaggccc agattgattt
    22621 cgcgggaaaa gctcatccgg taagcggtcg ccagccaggc gatgtagccc accagtaaac
    22681 ttagcagcac cgccagcggt aattgtgttg gaagatagtg ccatgccagc gccgtagccc
    22741 ccggaccatt aacgttaatg gtaaaaggaa agcgttgcga agaaacctga tagttttcat
    22801 tgtcttcagg ttgcggaagg gaatctacca gcccattacc atagagcaaa tgccgtttgt
    22861 ccaccgtcag gctggcgcta ctgatttgcg gcagttgtgg ctcaaggagc attgccgtca
    22921 gtaagtcgat gttaatcatc tccatgaccc cagcattgct actgcccgct gcaggcgtcc
    22981 attgaatcaa aaccggactg cctttaatca gggcacggtc gatcgtcagg cgtaaaagtg
    23041 gttgcggtgc aggaagttca gccagaatgt ccacgacggg cacattgcga taaccaaaaa
    23101 tgctggagca atagagtgtt ccgtcttgca ccaggccaat ggatcgcaca gtttggagtt
    23161 ttgccgcctg tttacgtaat ggcaaatggg cgacagagca gggcagacca atcagcggaa
    23221 gcaagacttc gctacctgcc tgtaggggaa gcagtacttt atctaattcc tctacagcgt
    23281 gattggcgaa ttgtactacc cgttgttgat ttaagttacg ctgtgaaata aatcgcacgc
    23341 tcagggtcga agcgagcgta acgagtgcgc aaatcgtaca aacaatcatt cgattacggc
    23401 gataggtttt aatgatccgt tgtgcttttt gcatgcgggc caccctgtaa gcgtatggcg
    23461 acaaacgcct gacgcaacag tggaagtgta gtggggaata ttacagcaga cgagaaagcg
    23521 ggtataaatt cgcccatccg ttgcagatgg gcgagtaaga agtattagtc acactggact
    23581 ttgattgcca gaccaccgcg tgaggtttcg cggtatttgg cgttcatgtc cttaccagtt
    23641 tcgtacatcg tttcgatgac cttatccagc gagacgcgcg gtgcactggt gcggcgcaga
    23701 gccatccgtg cggcgttaat cgccttcaca gaggcaatgg cattacgctc aatgcacggc
    23761 acctgaacct gccctgcaac cgggtcgcag gttaaaccaa ggttgtgttc catgccaatt
    23821 tccgccgcca cgcaaacctg ttccgggcta ccgcccagca gttctgcaag acccgcagca
    23881 gccattgaac aggcaacacc cacttcgccc tggcaaccaa cttccgcacc ggaaatagag
    23941 gcgttcattt tatacaatgc accaatcgcg cccgctgcca taaagtaacg ggtatagatg
    24001 tccgggctga ccgattcaat aaagtggtca tagtaagcca gcactgccgg aacgataccg
    24061 caggcaccgt tggttggcgc agttaccaca cgaccaccgg cggcgttttc ttcgttaact
    24121 gccagcgcaa acatgtttac ccagtcaatg acattcatcg gatcgttaga cagtttatcg
    24181 ctggaaacta acatccggcg cagggcagac gcacgacgtg gcacgcgcag cgggcctggc
    24241 agtacacctt cggtgttcat cccgcgatcg atacatgcct gcatggtttg ccagacatgc
    24301 gcgaaatact cgtcgatctc tttcttgctg tgcagcgcca gttcgttctg catagcgaga
    24361 ccagacagcg aatagccggt ttcattacag tacgcgagca gttcggtggc agatttgaac
    24421 ggatacggca cgctcacttc gttggcagca tcctgaccaa agtgttcttc atcgacgata
    24481 aaaccgccgc cgatggaata ataagttttg ctgtagacga cttcatcgcc gttataggcg
    24541 tggatttgca taccgttttc atgcagcggc aggttgccgt tatgaaaacg catcccgttg
    24601 tcgcgcggga aatccacttc atgccgtccc tgtgccagca gcagacgttc gcgctcttct
    24661 acgtcgcgaa taaaaccggg aatactgtcg atatccacgg tggcaggttc gttacctgca
    24721 agacccataa taatggcgat atcggtgtgg tggcctttac ccgtcagcga cagtgaacca
    24781 taaacgtcca cggcaacgcg agtaacgcta tccagtaagc ctttttcgac cagatcatcg
    24841 acgaactgtt tacctgcctt cataggccct acggtatggg aagatgaggg accaatcccc
    24901 accttaaaca tgtcgaatag actaatcacg ataatactcc tgacaaggtg actggacttc
    24961 cagtaacgaa ctaataacag cgcatagtgt aagagggaac gcggcgactg gcttaactat
    25021 tcacatgaat taaactaatc aaaaaccgcg atttaactaa tattgcgatg attgtctcaa
    25081 gtagtgtttt gtagatgtaa agataagtat agtcagggtt tcacaccaat ttgcagcgcc
    25141 agctcacgaa ttatgcctgc ggtcattccc catacaaaat actgttcgta ccaggacagc
    25201 catacccgat gtgaatcgcc acggcggtag atatctaaag ggtgataacg acccagatgt
    25261 aatgcctggg cgagcggcat ttcaaacacc gccgagactt catcttcact ggcgcgatac
    25321 ggcagatcgg gcgggataat gccgaccact ggggttacct gatagccagt tacgctatcg
    25381 acgggcggca gcacgccgat aacttcaacg gcggaaggcg gtatagcgac ctcttcttca
    25441 gcttcgcgca gcgcggcggc gataactgat gcgtccgtgt catcgactgc acctccaggg
    25501 aatgccactt gtccagcgtg tttacgcaga tgaattgaac gctgagtcag caacaacccc
    25561 ggttgcggtc gacggacgat ggggattaac acggcagcct gacgatgatt tagggtttcc
    25621 cggttaattt gcgggcgcaa aagttgaaag cgcgataaaa aatcatcaag catcaggcta
    25681 cggtattcca cgtcttactt ctccagttgg cgtaatatct tattaacttt atcaaaagtt
    25741 tcctgatatt ccgcttcttc ctggctatcg gcgacaattc caccgcccgc agagcagtat
    25801 atttgtccgt taatggcagt cagagtgcgg atagtaatgc tggtatccat gttgccgcaa
    25861 aagctcaaat agccaatgct gccgcaccag gcattacgtc gctgcggttc cagttcgtcg
    25921 ataatttcca tagcccgtac tttcggagcc ccggttattg agccaccagg aaaagctgcg
    25981 cgcagcagat cgctcgcgtg taactgttct ggtagttgtg ccgttatggt gctgaccaga
    26041 tgatgcacgg cagggaaggg ttccaccacg aacagctctg gtacttttac cgaacctgct
    26101 acggcaacac gaccgatatc attacgcatt aaatcgacaa tcatcagatt ttcggcacga
    26161 tctttcgctg agttcgccag tttttctgct tgtttgctat cttcctgagg atcgggcagg
    26221 cgtggtagcg tgcctttaat agggcgggtc tggatttcac tattatcaca aagaataaac
    26281 cgctctggcg aaaggcttaa aattgcaccc tgttcaagac gtaaaaaagc gctaaatggc
    26341 gcgcggttgg cctgattaag ctgaaggaat gcctgccatt catcgccaga ataggtcgca
    26401 tgaaaacgct gggcgagatt tacctgatag caatcaccgc tgtgcagata ttcctgtacc
    26461 tggcgaaatt tttcgccgta ctgctcgcgg gtcatattgg attgccagtc ggaagtaagc
    26521 gtgaaatctt cctgcggcga gaattgctgg ctttccagcc aggcccgacg ggcattgaca
    26581 tcattatgac tcagcaaaga aactgtatga cgctggtggt cgacaatgag cgcccaatcg
    26641 tagataccca ctgccatatc cggcagaacg atatcttgtt ccgcaatttc tggcagtgac
    26701 tcaaaacggc ggcccagatc gtagccaaac aaccccagtg cgccgccctg aaatggcaaa
    26761 tcttcgttat gcgttgggcg aatgtctgcg cgatccagca cctgctggag cacctgtagc
    26821 gggtcatcag tggtcgttgt gcgtttttcg ctttcactaa caacggtttc tttacctaaa
    26881 gtggttaaag tgcaaatcgg atcggcgacc acaatatcaa agcggctata cggatgatcg
    26941 gcatagccgg agtgtaaaag catcgcccac ggcaggtggc ttaagcggga gaaataaaat
    27001 tcagcggcgt cctgacgcca gggtaaagta atcacagcgg gagataacgt cttcatcagt
    27061 cctgactcta ctggctatgt gccgggtagc atgaaataat tagcgccaac aatttagcag
    27121 gagttaacaa tgtttgcagg tttaccttca ctcacccatg aacagcagca aaaagctgtc
    27181 gagcggatcc aggaactgat ggcacaaggg atgagcagcg gccaggcaat cgcgttggtg
    27241 gcagaagagc tgcgtgccaa ccattccggc gagcgaatcg tggcgcgttt tgaggatgaa
    27301 gac
//
LOCUS       DAKISA010000050        27120 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.050, whole genome shotgun sequence.
ACCESSION   DAKISA010000050 DAKISA010000000
VERSION     DAKISA010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27120
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.050"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..114)
                     /gene="gldA"
                     /locus_tag="OVW45_002249"
     CDS             complement(<1..114)
                     /gene="gldA"
                     /locus_tag="OVW45_002249"
                     /EC_number="1.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312901.2"
                     /note="forms dimers and octamers; involved in conversion
                     of glycerol to dihydroxy-acetone; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HCV5526682.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDK"
     gene            complement(125..787)
                     /gene="fsa"
                     /locus_tag="OVW45_002250"
     CDS             complement(125..787)
                     /gene="fsa"
                     /locus_tag="OVW45_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCV5526683.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(799..3300)
                     /gene="ptsP"
                     /locus_tag="OVW45_002251"
     CDS             complement(799..3300)
                     /gene="ptsP"
                     /locus_tag="OVW45_002251"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCV5526684.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLHTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSTLE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLRQHLLAGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            3609..4688
                     /locus_tag="OVW45_002252"
     CDS             3609..4688
                     /locus_tag="OVW45_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCV5526685.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGTWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            4703..5023
                     /locus_tag="OVW45_002253"
                     /pseudo
     CDS             4703..5023
                     /locus_tag="OVW45_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
     gene            5074..7370
                     /locus_tag="OVW45_002254"
                     /pseudo
     CDS             5074..7370
                     /locus_tag="OVW45_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
     gene            7336..8214
                     /locus_tag="OVW45_002255"
     CDS             7336..8214
                     /locus_tag="OVW45_002255"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCV5526686.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            8216..8557
                     /locus_tag="OVW45_002256"
     CDS             8216..8557
                     /locus_tag="OVW45_002256"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCV5526687.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(8544..9395)
                     /locus_tag="OVW45_002257"
     CDS             complement(8544..9395)
                     /locus_tag="OVW45_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCV5526688.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(9610..11343)
                     /locus_tag="OVW45_002258"
     CDS             complement(9610..11343)
                     /locus_tag="OVW45_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCV5526689.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(11526..14177)
                     /gene="ppc"
                     /locus_tag="OVW45_002259"
     CDS             complement(11526..14177)
                     /gene="ppc"
                     /locus_tag="OVW45_002259"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCV5526690.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(14529..15680)
                     /gene="argE"
                     /locus_tag="OVW45_002260"
     CDS             complement(14529..15680)
                     /gene="argE"
                     /locus_tag="OVW45_002260"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCV5526691.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDTLRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            15834..16838
                     /gene="argC"
                     /locus_tag="OVW45_002261"
     CDS             15834..16838
                     /gene="argC"
                     /locus_tag="OVW45_002261"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCV5526692.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            16846..17622
                     /gene="argB"
                     /locus_tag="OVW45_002262"
     CDS             16846..17622
                     /gene="argB"
                     /locus_tag="OVW45_002262"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCV5526693.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSTLVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            17683..19056
                     /gene="argH"
                     /locus_tag="OVW45_002263"
     CDS             17683..19056
                     /gene="argH"
                     /locus_tag="OVW45_002263"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCV5526694.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            19323..20240
                     /gene="oxyR"
                     /locus_tag="OVW45_002264"
     CDS             19323..20240
                     /gene="oxyR"
                     /locus_tag="OVW45_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCV5526695.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGYFDKVLKQAV"
     gene            complement(20223..21623)
                     /gene="sthA"
                     /locus_tag="OVW45_002265"
     CDS             complement(20223..21623)
                     /gene="sthA"
                     /locus_tag="OVW45_002265"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCV5526696.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGVRVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            21944..22594
                     /gene="fabR"
                     /locus_tag="OVW45_002266"
     CDS             21944..22594
                     /gene="fabR"
                     /locus_tag="OVW45_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCV5526697.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            22594..22953
                     /locus_tag="OVW45_002267"
     CDS             22594..22953
                     /locus_tag="OVW45_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCV5526698.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(22993..24093)
                     /gene="trmA"
                     /locus_tag="OVW45_002268"
     CDS             complement(22993..24093)
                     /gene="trmA"
                     /locus_tag="OVW45_002268"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCV5526699.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      24207..24397
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            24462..26306
                     /gene="btuB"
                     /locus_tag="OVW45_002269"
     CDS             24462..26306
                     /gene="btuB"
                     /locus_tag="OVW45_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCV5526700.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            26251..27108
                     /gene="murI"
                     /locus_tag="OVW45_002270"
     CDS             26251..27108
                     /gene="murI"
                     /locus_tag="OVW45_002270"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCV5526701.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         6196 a         6780 c         7491 g         6653 t
ORIGIN      
        1 tttgtcaccc accactaacc agcgttctgc cagcggcttc aggtattcgc ccagacgatt
       61 aatcacatca gcgccctgga tgtatttacc cggtgattga ataatgcggt ccataattgc
      121 tcctttagag atgagtagtg ccaaatgcgg cattccagtc gtgttcgaac ttctctatag
      181 ctgactctac cgcaggggtg ttgagcattt gttgcgctac atctaagggc agggtgatgg
      241 attcacatcc tgccagtaaa cagtccagcg cctgacgcgg cgttttaaag ctggctgcca
      301 gcaccatgct ttctggcgcg tgcatttcta acagcgcttg cagctcctga accgtacgaa
      361 tgccgtctcc gccctgggca tctacgcggt taacatacgg agcaacgtat tttgcccctg
      421 ccagcgcggc cagtaaccct tgtgcagcac tgtacactgc ggtgccaagc gtagtaatac
      481 cctctttttt cagtatttta attgctgcca gaccttcgga agtcaccggg attttcacca
      541 caatacccgg aatagcgtcg cgcaggcgct tcgcttcttc caccatcccc tgcgcgtcgc
      601 ggctcatggt ctgagcaaac agaatgccct catcaccaat cgctttttgc agacgcggca
      661 gcacttccca tatggactcc ttgctggcag cgataatgct cgggttagtt gtcaccccgg
      721 caatggggaa tatgcgtgcc agacgttcga cttctgcgac gttagcggtg tccagataca
      781 gttccatgat gtttcctttt acagttccag ttcatgttgc agcaggctgg cgatagcgtc
      841 ggcagaggcg gcgttaacca gcgcgttacg gaattcttcg tgcatgatgc ggcgagcgag
      901 gcgcgagaaa atgcgcatgt gctgatcgcc cgcagcgtgt ttgttcaggg ttaacatgat
      961 gatgaattgc gcttcatcat caccccagcg caccggcgct tgcagacgcg caacgctgat
     1021 ggtggattgc tcaatgtgtt ctgatttgct gtgtggaatg gcaaaactaa agcccagacc
     1081 ggtagagaaa acggcctcgc gcgcccacaa gtcggcttcc agtttacgcg gatagcggca
     1141 gcgccctgcc agcagcaggt tatcggtcat gcctttgagc acttcttctt tgctgcgcca
     1201 gtcgctttcc agtgtgatgc actcggcggt gaccagcggt gcgtcttgtt gggtcatgcg
     1261 gaattgcgcc agcaggtgtt ccacttccag cgaagtacgg caggccattg cctggttgag
     1321 caacttgcgg cactcacggc tatcaagttg cgccatccgc gctttcgccg ccggaattga
     1381 tggtgcgctc atgctgagtt catccagccc taagccgacc agcaacggca gcacggaacc
     1441 tttcgctccc agctcaccgc acagaccaat ccatttaccc tggcgatgca ccgcctgcac
     1501 ggcgtaatcg agcgcccgca agaatgccgg attcaggctg ttgtagtgac gagtaacctt
     1561 agcgttatcg cgatccactg ccagcagata ttgcgtcagg tcattactac caatactaaa
     1621 gaaatcaatc tcttcgcagc attgatcgat gatgaacatc accgacggca cttccagcat
     1681 aatgccgagc tggatcttct catcaaacgg aatgtgttcg ttacgtagtt gctgtttggc
     1741 ttctgccagt ttttctttca cccataagat ctcttccatt gaggagatca tcgggatcat
     1801 gattttcagg ctgccgtgag cggaggcgcg caggatcgag cgtagttgtg tggtaaataa
     1861 cgacgcgtat tcttcataaa tacgcacggc gcgataaccg aggaacgggt ttgcctctgc
     1921 gggaatgttt agataatcaa cgggtttgtc gccgccaatg tccatagtgc gcacaataat
     1981 gctgcgtccg ttggcagact ccagcgcctg acaaaaaatg ttgtacagct cgctttcgcc
     2041 cggtgcactg gtgcgatcca tatagagcat ttcagtgcgg aacaaaccaa cgccttccgc
     2101 accattgccg aatgcggcct gcgcttccac ggagtgagcg atgttagcgg caatttcaat
     2161 gcggataccg tcagcggtac gggcttgttg ggtcagccag acacgctgtt gctcacgcag
     2221 ggcgtcctgt acgcgggctt cttgctgata ataacgagct acggcttctc ctggctcaac
     2281 cacaatcgcc ccggcgttgc cgtcgatata aatcgtttga tgctgccacg gagtgagggc
     2341 atcaatatcc acaccaacca gcgtcggaat gttgaacgaa cgggcaagga tcaccgtatg
     2401 tgaggtggtg ccgccacttt tcagcaacag acctttgagg tgatttttat ccagttcgag
     2461 gaactggctg ggggtcagtt catcagccat acaaatggcg gactgcgtca gtttgcccgg
     2521 tgccgggaag cgttgctcac cgtagatttg ctggagtaac tggaagcaga cgtcgcgtac
     2581 gtccagggca cgttcttgca ggtagctgct gctggaacgg gcgaactctt cgcaaaagtg
     2641 attcgcactg gcgacaatcg cttcggcgca gcttaatccc tcgctgacgc ctgccagtaa
     2701 gtgctgacgt aaagatgtat cgccagccag ggatcggtga gcttccagta tcgcgctggt
     2761 agcaccgtcg ctatccagca gacgaaactc aatgtttttc agtaccagcg tcaggccgtt
     2821 ttccagtgtg gattgctcgg cgtcaacgtc tttggctgcg ggaagattac tcagcgcatt
     2881 gagatctaaa gaagagatcg gcgtcagaat gccgcctgca ctaccgctgc acacggtgcg
     2941 ggcgcggata atttgcggat tcagattggt cagtgaaacc ggcagtggtt ccagttcgtc
     3001 agatttaact tccgccagcg gcgcatcgca gtgggggaat tcatcgcgca gccattggct
     3061 taaacgctgg tgcgcttcct gttcgtcggc cccggaaatc agtaactggc agttatcgcc
     3121 cgccagcgta tcggtgccaa tcagcgcaag ggcgcttttg gcgttgccct tgcggtcagt
     3181 gtgcaggtta tgccactcaa tttgtgatga aaaagtatta cacagcgttt caacgtggct
     3241 tgccggacgc gcatgtacgc cgttaggtag ctcacaaata aattccacaa tcagggccat
     3301 tgcctctctc ccggtacggt tttctgttta caggataaga gcgcacggca acggcctgcc
     3361 atgtgacaaa tctgccaaaa gctggacaaa tgtaatgtaa ccgtcaattt gcgacgcgtc
     3421 tcacaagacg ccgttttgcg gcatgcttcc ggtttatcgc aagttatgag gcggatcgca
     3481 tttttgtact gatattacaa aagtccagta aatggccttt ttatccactg tttgctccgc
     3541 ctgcgattgc ctattgtttc ctccatcaaa aatatggaca tgggctttcg caagcccggg
     3601 agcagcttat gaatgagttg gtgcagatcc tgaaaaatac ccgtcagcat ttaatgacgg
     3661 gcgtttcaca catgattccc ttcgtggtat cgggcggtat tttgctggcg gtttccgtca
     3721 tgttgtatgg caaaggcgca gtgccggatg ccgtagccga tccaaatctg aaaaaactgt
     3781 ttgatatcgg cgttgcgggc ttgacgctga tggtgccttt cctcgccgct tacattggtt
     3841 attccattgc agaacgttct gcgctggctc catgcgctat cggtacctgg gttggtaaca
     3901 gctttggtgc gggcttcttt ggtgcactga tcgccgggat tatcggcggc atcgtggtgc
     3961 attacctgaa gaaaattccg gtgcataaag ttctgcgctc ggtgatgcct atcttcatta
     4021 ttcctatcgt cggcacactg attaccgcag gcatcatgat gtggggcttg ggcgagcctg
     4081 taggggcgtt gaccaacagc ctgactcagt ggcttcaggg gatgcagcag ggcagcattg
     4141 ttatgctggc ggtgatcatg ggtctgatgc tggcgttcga tatgggcggt ccggttaaca
     4201 aagtggccta tgccttcatg ctgatttgcg ttgctcaggg tgtttatacc gtggtggcca
     4261 tcgctgccgt gggtatttgt atcccgccgc tggggatggg gctggcgacg ctgattggtc
     4321 gtaaaaattt ctccgcagaa gagcgcgaaa ccggcaaagc ggcactggtg atgggctgtg
     4381 ttggcgttac tgaaggggcg attcctttcg ccgctgccga tccgctgcgt gtcattcctt
     4441 ccatcatggt cggttcagtt tgtggtgcag taactgcggc gctggtcggt gcgcagtgct
     4501 atgcaggctg gggtggtctg attgtgctgc cggtggttga aggcaagctg ggttatatcg
     4561 cagcagtggc tgtcggagca gtggtgacgg ctgtttgtgt gaacgtgctg aaaagtctgg
     4621 cgcgtaaaaa tgggtcttcg actgatgaaa aagaagacga cctggatttg gattttgaaa
     4681 ttaattaatt gaggaacgag ccatgacgaa aattattgca gtaaccgcat gtccttcagg
     4741 tgttgcccat acctatatgg cagcagaggc gctggaaagt gccgcgaaag cgaaaggctg
     4801 ggaagtgaaa gtagaaacct aggggtcaat tggtctggaa aatgaactga ctgcggaaga
     4861 tgtggcgagc gctgatatgg tgattctgac caaagatatc ggcatcaagt ttgaagagcg
     4921 ttttgctggc aagaccatcg tgcgcgtcaa catcagcgat gcagtaaaac gtgctgacgc
     4981 cattatgagc aaaattgaag ctcacctggc gcaaaccgct taagttcttc tcccgctcgc
     5041 aagggcgggt tcgctttccc acaggagttc ctcatgacga atcgtatctc tcgcctcaaa
     5101 actgcactgt ttgccaatac ccgtgaaatc tcgctggagc gggcgctgct ttataccgcc
     5161 agccatcggc aaaccgaagg cgaaccggtg atattgcgcc gggcgaaagc aacagcgtat
     5221 atccttgaac atgttgaaat ttcgattcgt gatgaagaac tgattgccgg taaccgcacc
     5281 gtaaaaccgc gcgccgggat tatgtcgccg gaaatggacc cttactggct gctgaaagag
     5341 ctggatcaat tcccgacgcg tccgcaggac cgctttgcta tcagcgaaga agataaacgt
     5401 atctaccgtg aagagttgtt cccgtactgg gaaaaacgtt cgatgaaaga tttcatcaac
     5461 gggcagatga cagatgaagt aaaagccgcg accaacacgc agattttcag catcaaccag
     5521 acggataaag gccaggggca cattattatt gattacccac gcctgttgaa tcacggtctg
     5581 ggtgagctgg tggcacagat gcagcaacat tgtcagcaac agccggagaa tcacttttat
     5641 caggccgcgt tgttactgct ggaagcctcg cagaaacaca ttttgcgtta cgccgaactg
     5701 gcggaaacga tggcggcaaa ctgcacagat gcacagcgtc gcgaagaact gctgactatt
     5761 gcggagatct ctcgccataa cgccgaacat aagccgcaga cgttctggca ggcgtgccag
     5821 ttattctggt acatgaacat cattctgcaa tacgaatcca acgccagttc gctgtcttta
     5881 gggcgcttcg accagtatat gttgccgttc tatcaggcat ctttaaccca gggcgaagat
     5941 ccggcgttcc tgaaagaact gctcgaatct ttatgggtga aatgcaacga catcgtgctg
     6001 ttgcgctcca ccagcagcgc gcgttatttc gcaggtttcc cgaccggcta taccgcactg
     6061 ctcggcgggt taaccgagaa cggacgtagc gcggtgaacg tgctttcgtt cctttgcctt
     6121 gacgcctatc aaagcgtgca attaccgcaa ccgaacctcg gcgtgcgcac taacgccttg
     6181 atcgacacgc cgttcctgat gaaaaccgcc gaaaccattc gcctcggcac cggtattccg
     6241 caaatcttta acgatgaagt ggtggtacca gcgttcctca accgtggcgt ttcgctggaa
     6301 gatgcgcgcg actattccgt agtgggctgt gtggaattat ctattcccgg cagaacctac
     6361 ggcttgcatg acatcgcgat gtttaatctg ctgaaagtga tggaaatctg cctgcatgaa
     6421 aatgaaggca atgctgcgct gacttatgaa ggtttactgg agcagatccg cgccaagatc
     6481 agccactaca tcaccctgat ggttgagggc agcaatattt gtgatatcgg ccatcgcgac
     6541 tgggcacctg taccgctgct ctcgtctttt atcagcgatt gtctggaaaa aggtcgcgat
     6601 attaccgatg gcggcgcgcg ttataacttc tccggcgtac aggggatcgg tatcgccaac
     6661 ctgagcgatt ctctccatgc gttgaaaggg atggtttttg agcaacagcg tttaagtttt
     6721 gacgaattgc tgtcggtatt aaaagccaac tttgcaacgc cagaaggcga aaaagtccgt
     6781 gctcgcttaa ttaaccgctt cgagaaatac ggtaacgata tcgacgaggt ggataacatc
     6841 agcgccgaac tgttacgcca ctactgcaaa gaagtggaaa aataccagaa cccgcgcgcg
     6901 gttacttcac gccgggatcg tataccgttt ctgctcacgt cccgttggga tcggtggttg
     6961 gcgcgacgcc agacggtcgt tttgccgggg aacaactggc agacggcggc ttgtcaccta
     7021 tgctgggcca ggacgcacaa gggccaacag cggtactgaa gtcagtcagt aagctcgata
     7081 atacgctgct gtctaacggt acgttgctga acgtgaaatt cactccggcg accctggaag
     7141 gtgaagcggg attacgcaaa ctggccgact tcttacgggc gtttacccag cttaagttgc
     7201 agcatattca gtttaacgtg gtgaacgccg acacgttgcg ggaagcgcaa cagcgcccac
     7261 aagattatgc cgggctggtg gtgcgcgttg ccggatacag cgccttcttt gtcgaactgt
     7321 cgaaggagat ccaggatgac atcatccgcc ggacagcgca tcagctgtaa cgttgtggaa
     7381 acgcgccgcg atgatgtggc gcgcattttc aacattcagc gttattcact gaatgacggc
     7441 gagggcattc gtacggtggt cttttttaaa ggctgtccgc atctttgccc gtggtgtgct
     7501 aatccggagt cgatctccgg caaaatccag acggtacgca gagaggcgaa atgtctgcac
     7561 tgtgcgaaat gtttgcgtga tgcggatgaa tgcccctccg gggcgtttga acggattggt
     7621 cgcgatatca gccttgacgc tctggaacgg gaagtgatga aagatgacat tttctttcgc
     7681 acgtccggcg gcggcgtcac gctttctggc ggcgaagtgt taatgcaggc ggagtttgct
     7741 acccgttttt tacagcgact gcggctgtgg ggtgtgtcat gcgccattga aactgccgga
     7801 gacgcgccag caagcaagct gttaccgctg gcgaaattgt gcgatgaagt gctgttcgat
     7861 ttaaaaatta tggacgcgac tcaggcgcgg gatgtggtga agatgaacct gccacgcgtg
     7921 ctggagaatc tgcgtttgct ggtgagtgag ggtgtcaacg tgatcccgcg tttaccgttg
     7981 atccctggtt tcacgctcag cagggagaat atgcagcagg cgctggatgt actgatcccg
     8041 ctgaaaatca ggcagatcca tctgttaccg tttcatcagt acggcgaacc gaaataccgc
     8101 ctgctgggga aaacatggtc gatgaaagag gtgcctgcgc cgtcgtcagc cgatgtgaca
     8161 acgatgcgcg aaatggcaga acgggccgga tttcaggtta ccgtgggagg ttaaaatggc
     8221 atacctggtg gcagtaaccg cctgcgtaag cggcgtggcg catacttata tggcggcgga
     8281 acggctggaa aagttgtgcc agttagagaa gtggggcgtc agcattgaaa ctcagggcgc
     8341 gctgggaacg gagaatcgtt tagcggacga ggatatccgt cgggcggatg ttgctctgtt
     8401 gattacggat atcgagcttg ccggtgccga gcgatttgaa cattgccgct atgtgcaatg
     8461 cagcatctac gcattcctgc gtgagccgca gcgggtaatg agcgcggtgc gcaaagtgct
     8521 ttctgcgccg cagcaaaccc atcttattct ggagtagtcg gtttttctgt cagttggctg
     8581 tggtactgcc ggcgatattc cgacggcgag cgttcggtgt ttttacgaaa cagacggcag
     8641 aagtagttgc tgtcgacaaa gccgcaggcg tgcgccactt cttttacctt caggtcatag
     8701 cctttcaata acgttttagc gtgctccagt cgcgtgtgat tcaggtattc gttaaaacca
     8761 atggcccccg ttttttgaaa caggtgcgag agatagtttg gtgaaatata gaatgcctgt
     8821 gcgacagatt cgcgggtaag cgcggaggca tagcgttcgt cgatataatc gcgaatagct
     8881 tcaaatagtg cctggctgcg tgaggcggtc tggatttggc tgccgagcaa atcgcggcag
     8941 tggctaagca ggctggctac gataagcctt gccgtttgct gctcctgcgg ctgcatttgc
     9001 atttcattga gcgtttgtag cagaaaagaa cctatacgtg ggccgcgtcg ggcgacgtgt
     9061 tgcttcgcca gattttgata ttgtttgcca tcccattgca cgacgctgaa gccgagttgc
     9121 tgtttgccaa acagcacgct aaaggtggta gcgggtgttt tccattgtgg gaaattccag
     9181 ccaccagcgg caacgtacag cacatcgccg ggaactaacg ttgctccagc gccggtatca
     9241 acaaactcac cttccagcac aatttccagc cgtggaaaat cgacctgata cgcaagatcg
     9301 ggcaccgggc cgttactgct ggcaaagtaa atctggcgca gggacagcgg gccgttgatc
     9361 aggcgggaga gcagatagct gacgtcgtga tacatgttat tcccttaggt atgtagacgg
     9421 gaaaacagta actggcatta ttaaaaaagg ccacctgaaa agtgtggcct gaggggagtt
     9481 cgatgtaaac attgcggcag ttgtattgcc ggatgtggcg taaacgcctt atccagccta
     9541 ccgccgcgat ctgtaggccg gataagacgc gtcaagcgtc gcatccggca aatagcgcct
     9601 ggctgataat tactgattac ctacctggtc gccatacggc agagtgtcgt aatcgatcag
     9661 cgcgtttttc ttgtacgggt taccaatcca gcgggtagtt tctttgaact gcggattcac
     9721 cactgaacgg gttggatcgt aaccgtcgta agataagccc gccaaatctg accaggtgtg
     9781 gatcagttcc gccaggctgt atttacgatc gacatcctgt gagaaatcac ggggatgagt
     9841 cgcttgccat ttttccgacg tccacagcag gaacggaatg gtgtacatgt ggcgcgtcgg
     9901 gttgtcttcg ttacgcccct gagttttgtg cggcggcgtg tcgtaaacct cttcaccgtg
     9961 gtcagagaag taaaccagga atccgttcgg gtctgccgct ttaaagtctt taatcaggct
    10021 ggcaaccaca tgatcgttat acaggttagc gttgtcataa tcgttatatg attccagctc
    10081 ttctgcgctt aatcctggcg gaacatgatc ggtattgcca tcaaacttgc cctgattttc
    10141 cgggtagcgg tatttgtatt tgatatgcgt acccagcaga tgaacgatga tcagtttctt
    10201 cggcgcaggg tcattcagca catcctggaa cggcttcagc acgttggtgt cgtattcacg
    10261 cgcactctgc gtacgttgct ggttcatgta gtactgcttg tcggtctggc gcgaaaatac
    10321 cgtcagcatg gtattgcggg cggtcatcgt ctgctggttg gtgatccaga aggttttata
    10381 acccgcctgt ttcatcatgt tcatcagcga cggctgcgtc agatacagat ccgggttctt
    10441 ttcattggca aaggtcagcg cctgttgcag gatttcaatg gtgtacggac gagacgtaac
    10501 tacgttatta aacacggtca gattcggatc ggttttatgc agcgcatcca gctccggcgt
    10561 ggtttcacgc ggataaccgt acagactcat gcgtccgcgc tgggtcgact cgccaatcac
    10621 cagcactaaa gtgcgcggtt cgttacccga ttcatctttg aaattagcca gtggcggcaa
    10681 ggcattattt tcattcagta actttgttag cgagtttagt tgctgacgat actgataata
    10741 gccggtcagg aattgccacg gtgcggcagg ctccatacgc gaggccaggt tatccaacgt
    10801 tttctcaaac ggcttgtttt tgataaacgt attcatggcg atcggatgca gaatcaagcc
    10861 ataaagcagg gcaaaagaga caacataacg ccacggcttt ggaatataga ccgggcgcag
    10921 gcgtgtccac agcagaactg ccaccgccgt ataggccagc gcgataagca caattttcag
    10981 gctgaaatac tggcttaaat actcgctggc ttcgttggtg ttggtttcga acatcacaaa
    11041 cagaacgctc tgcgagaact cctgcccgta gatgacgtag tagcatagcg ccgccagaga
    11101 ggccgcccat agcaccacgc cgattactgc ggcaataatt ttaatccgct tcggaaagag
    11161 gaataccggg atcaaccaca gcgagctgaa taacagcgag tcgcgaatgc cgttggtgcc
    11221 actataacca ctgatgtaaa taatggcctg tagcagagta gagaaaaacc aaaagtagag
    11281 cagtgcccaa cccagggctt tccagctaaa aaggggttta gcctggactt ctgtggaatg
    11341 catagtaaga acctgtcttg aaaaaaatat cgccgaatgt aacgacaatt ccttaaggat
    11401 atctgaaggt atattcagaa tttgaataaa atgcagacag aaatatattg aaaacgaggg
    11461 tgttagaaca gaagtatttc agaaaaccct cgcgcaaaag cacgagggtt tgcagaagag
    11521 gaagattagc cggtattacg catacctgcc gcaatcccgg caatagtgac cattaacgct
    11581 tgttcgacgc gaggatccgg ttcctggcct tctttttctg cctggcggga gcggtgcagc
    11641 aactcggcct gcaatacgtt cagcgggtcg gtgtaaatat tccgtagctg aatagactct
    11701 gcaatccacg gcagatcggc catcagatgg gaatcgttgg caatcgccag caccactttg
    11761 atgtcttctt cttgcaggtt gcgtaactct ttacctaacg gccacagtgc tttgtctacc
    11821 agacgttgat catagtattc cgccaaccac aggtctgctt tggcgaagac catctccagc
    11881 atgccgagac gcgtcgagaa gaatggccaa tcgcggcaca tggcttccag ctcgctctgt
    11941 ttgccatctt cgaccacttt ttgcagcgcc gtacctgcac ccagccaggc ggggagcatc
    12001 aggcggtttt gcgtccaggc aaaaatccac ggtatggcgc gcagcgactc gacgccgccg
    12061 gttgggcgac gtttcgccgg acgtgaaccc aacggcagtt tgcccaattc ttgttccggc
    12121 gtagcggagc ggaagtaagg cacaaaatct ttgttttcac gtacgtagcc acggtagaga
    12181 tcgcaggaga tgactgacag ttcatccata atgcgacgcc agctctcttt cggctcaggt
    12241 ggtggcagca ggttggcttc cagaatcgcc ccggtgtaaa gcgacaggct gctgacggtg
    12301 atttctggca gaccatattt aaagcggatc atctcgccct gttcagtcac gcgcaggcca
    12361 cctttcaggc ttcctggcgg ttgtgacagc agcgccgcat gggcaggtgc gccgccgcga
    12421 ccaatggaac cgccgcgacc gtggaacaac gtcagctcaa tacccgcttt ttcgcaggtt
    12481 ttgattaatg catcctgtgc ctgatattgc gcccaggaag ctgccatcac gcccgcatct
    12541 tttgctgagt cggaatagcc aatcatcacc atctgtttgc cctgaatcag gccgcgatac
    12601 cagtcgatat tgagcagctg ggtcatgaca tcgttggcgt tgttcaggtc atcgagggtt
    12661 tcaaacagcg gagcaaccgg catcgcaaac ccgatacccg cttctttcag cagcaggtgg
    12721 acagccagta cgtcggacgg cgttttcgcc atcgagatca cgtaggcggc aatggagcct
    12781 tgcggcgctt cggcaatcac ctggcaggta tcgagcactt cgcgcgtttc ggcgcttggt
    12841 tgccagttgc gcggcagaag cggacgtttg gagttcagtt cgcggatcag gaacgcctgt
    12901 ttgtcggcct ctgaccagct ttcgtagtcg ccgataccga ggtagcgggt cagctcgccc
    12961 agcgcttcgg tatggcgggt gctctcctga cggatatcaa tacggaccag cggtacgccg
    13021 aaacatttca cgcggcgcag ggtgtcgagc agatcgccgt tggcgataat acccatgcca
    13081 cacgcctgaa gtgactggta gcaagcgtag agcggttccc acagttcttc gttttgtgtc
    13141 agcagccctt ctggttttgg cagttcttcg cctttcaggc gcgcttccag ccatgcctgt
    13201 gtcgccatca ggcgagaacg caggtttttc atcagatagc gatacggttc tgcggcacct
    13261 tcttcgccaa ccagcgccag cagttcaggg gtcgcttcaa ccatcgacag ttcagaaacc
    13321 agaacctgaa tatctttcag gaacagatcg gtggctttcc agcgactgag tagcaggacg
    13381 tggcgggtga tatcggcagt gacgttcggg ttgccgtcgc ggtcaccgcc catccacgaa
    13441 gtaaaacgga ccggaacaaa ttcgacgggc agtttgtagc cgaggttctc ttccagttgt
    13501 tcgttcagtt cgcgcaagta attcggtacg ccttgccaca ggctgttttc tactacggca
    13561 aagccccatt tggcttcatc taccgggctt ggacgcagct tacggatttc atcagtatgc
    13621 catgactggg cgattaactg gcgcaggcga cgcatcagct ggttgtgttc gtagtcagcg
    13681 atatctttgt tatcgagctg ttttaagcag gcgttcactt ccaccatttt gtggatcagt
    13741 gtacgacggg taatttcggt tgggtgagcc gtgaggacca gttccagcga cagcgattcc
    13801 actgcttttt tgatggtgtc ttcgctcagt tccggctggt ttttcagttt gcgcagggtg
    13861 cgggcgatca cttccgggtt gctggcagct tcgcctttcg gcgaaatgct gtggtattgc
    13921 tcggcggtgt tggccaggtt caggaactga ctaaacgcac gcgcaacggg cagcagctcg
    13981 tcgttcgaca aattttgtaa ggtggtgagc aactcctggc ggttagcatc attgccagcg
    14041 cgtgaagatt tcgacaactt acggatagtt tctacgcgtt caagaatgtg ttctcccaac
    14101 gcatccttga tggtttctcc cagcactttg ccgagcatac tgacattact acgcaatgcg
    14161 gaatattgtt cgttcatatt accccagaca ccccatctta tcgtttgata gccctgtatc
    14221 cttcacgtcg cattggcgcg aatatgctcg ggctttgctt ttcgtcgtct tttataaagc
    14281 cacgtaaaag cggtgacgtc aaatgctgcg aaatcgcttc agcaaacgaa taaatagcag
    14341 gaatttacgc cattaaattc acgacgcttt aaataagcgt aacttatgga aatgttaaaa
    14401 aatcgccata cataacacca aaggtgtagg tcggataaga tgcgcaagca tcgcatccga
    14461 cattattgcg gcaccggagt ttggcaacgg tgccggatgc ggcgcgagcg ccttatccgg
    14521 cctacgtttt aatgccagca aaaatggtga attacctgga taatcaactc acgggtgggt
    14581 ttaataaacc gcgtctccag atattcatca ggctgatgag cttgattgat ggaacccgga
    14641 ccgagcacca gcgtcgggca taacgtttga ataaacggcg cttcggtaca gtaattcacc
    14701 acttcggttt ttgctccgag caatttctca accacttcaa ccagttgatg attcggtggg
    14761 cattcatagc cagggatcgg cggatgcagc tcgtcgaccg tcagacgacc cggccagcgt
    14821 tcgctcaccg gagccaatgc atcgttgagc aaaccattaa gttcattgag tgtcatgcca
    14881 ggcagcggac gaatatccat atgcaactca cagcaagcgc aaatacggtt agaagcgtcg
    14941 ccaccgtgaa tatgcccgag gttgagcgta gggtatggca cggtaaatgc ttcgtagtga
    15001 taacgttctt tcaggttatc gcgcaattgc aaaatatgcc cgatggcgtc gtgcattagt
    15061 tcgatagcgt taactccgcg tgctggatcg ctggagtgcc ccgactggcc ctgaatacgg
    15121 atggcgttag agatatgacc tttatgtgcg cgtaccggtt gtagtgacgt cggttcgcca
    15181 atgatggcgc aatccgggcg cagggcggta gtttcggcaa aataacgcgc tccggccata
    15241 ctggtttctt catcagcagt cgccagaatg tagagcggtt ttttcagttt cgtgacgtcg
    15301 acatcgcgta gcgtatcaag gataaacgca aaaaagcctt tcatgtcggc ggtgcctaag
    15361 ccgtaaagct tgccgtcatg ctccgtcagt gtaaacggat cgcgcgtcca gcgaccgtca
    15421 tcaaatggca ccgtatcggt atgccccgcc agcaacaagc cgccagcccc ctgtccgata
    15481 cttgccagca tattgaattt gttgcgagtt cctggcacag gctgcacttc cacattgaag
    15541 cctaaatctt taaaccagtc cgccagcaga gtgattaaat ctgcattgct ttgatcgagt
    15601 gcctcttccg tggcgcttat tgaaggtgtg gcaatcagag cacggtaaat ctcgataaat
    15661 ggcggtaatt tgtttttcat tgttgacaca cctctggtca tgatagtatc aatattcatg
    15721 cagtatttat gaataaaaat acactaacgt tgagcgtaat aaaacccacc agccgtaagg
    15781 tgaatgtttt acgtttaacc tggcaaccag acataagaag gtgaatagcc ccgatgttga
    15841 atacgctgat tgtgggtgcc agcggctacg ctggcgcaga gctagtgacc tatgtaaatc
    15901 gccatccgca tatgaacata accgctttga ctgtttcagc gcaaagcaat gatgcgggaa
    15961 agttaatctc cgatttgcat ccgcagctaa aaggcatcgt tgagctgccg ttgcagccga
    16021 tgtcggatat cagcgagttt agcccagggg tggacgtagt gtttctcgcc accgcccatg
    16081 aagttagcca cgatttagcg ccgcagtttc ttgaagcggg ctgcgtggtg ttcgaccttt
    16141 ccggtgcgtt tcgtgttaac gacgccacct tctatgaaaa atattacggc tttacccatc
    16201 aatacccgga actgctggaa caggcagcct acggtctggc ggagtggtgc ggtaataaat
    16261 taaaagaagc gaatttgatt gcggtgccag gctgttatcc gacggcggca cagctggcgc
    16321 tgaaaccgtt gattgatgcc gatcttcttg acctcaatca gtggccggtg atcaacgcca
    16381 ccagcggcgt gagcggtgca ggacgtaagg cagcgatttc aaatagtttt tgtgaagtta
    16441 gcctgcaacc gtatggcgtc tttactcatc gccatcaacc agagatcgcc acacacctcg
    16501 gtgctgacgt tatcttcacc ccacatctgg gcaatttccc gcgcggcatt ctcgaaacca
    16561 ttacctgccg cctgaaatcg ggtgtgaccc aggcgcaagt cgcgcaagtg ttacagcagg
    16621 cgtatgccca taaaccgctg gtgcggctgt atgacaaagg cgttccggcg ctgaaaaatg
    16681 tcgttgggct gccattttgc gatatcgggt ttgccgttca gggcgagcat ctgattattg
    16741 tggcgaccga agacaactta ctgaaaggcg cggcggcaca agcggtacag tgcgccaata
    16801 ttcgtttcgg ctatgcggaa acgcagtctc ttatttaagg gtgcaatgat gaatccatta
    16861 attatcaaac tgggcggcgt actgctggat agtgaagagg cgctggaacg tctgtttagc
    16921 acactggtga attatcgtga gtcacatcag cgtccgctgg tgattgtgca cggcggcggt
    16981 tgcgtggtgg atgagctgat gaaagggctg aatctgccgg tgaaaaagaa aaacggcctg
    17041 cgggtgacgc ctgctgatca gatagacatt atcaccggag cactggcggg aacggcaaat
    17101 aaaaccctgt tggcatgggc gaagaaacat cagattgcgg ccgtaggttt gtttctcggt
    17161 gacggcgaca gcgtcaaagt gacccagctt gatgaagagt taggtcatgt tggactggcg
    17221 cagccaggtt cgcctaagct tatcaactcc ttgctggaga acggttatct gccggtggtc
    17281 agctccattg gcgtaacaga cgaagggcaa ctgatgaacg tcaatgccga ccaggcggca
    17341 acggcgctgg cggcaacgct gggcgcggat ctgattttgc tctccgacgt cagcggcatt
    17401 ctcgacggca aagggcaacg cattgccgaa atgaccgccg cgaaagcaga acaactgatt
    17461 gagcagggca ttattactga cggcatgata gtgaaagtga acgcggcgct ggatgcggcc
    17521 cgcacgctgg gccgtccggt agatatcgcc tcctggcgtc atgcggagca gcttccggca
    17581 ctgtttaacg gtatgccgat gggtacgcgg attttagctt aagttttgtt ggccggaggc
    17641 gcagctttcc ggcattgaat ttcaaaataa ggaaacagag ttatggcact ttggggcggg
    17701 cgttttaccc aggcagcaga tcaacggttc aaacaattca acgactcact gcgctttgat
    17761 taccgtctgg cggagcagga tattgttggc tctgtggcct ggtccaaagc cctggtcacg
    17821 gtaggcgtgt taaccgcaga agagcaggcg caactggaag aggcgctgaa cgtgctgctg
    17881 gaagatgttc gcgccaggcc acaacaaatc cttgaaagcg acgccgaaga tatccatagc
    17941 tgggtggaag gcaaactgat cgacaaagtg ggccagttag gcaaaaagct gcataccggg
    18001 cgtagtcgta acgatcaggt agcgactgac ctgaaactgt ggtgcaaaga taccgttagc
    18061 gagttactga cggctaaccg acagctgcaa tcggcgctgg tggaaaccgc acaaaacaat
    18121 caggacgcgg tcatgccagg ttacactcac ctgcaacgcg cccagccggt gacgttcgcg
    18181 cactggtgcc tggcctatgt tgagatgctg gcgcgtgatg aaagccgttt gcaggatgcg
    18241 cttaagcgtc tggatgtcag cccattaggc tgtggcgcgc tggcgggaac ggcctatgaa
    18301 atcgaccgtg aacagttagc aggctggctg ggctttgctt cggcgacccg taacagtctg
    18361 gacagcgttt ctgaccgtga ccatgtgttg gaactgctgt cggctgccgc tatcggcatg
    18421 gtgcatctgt cgcgttttgc tgaagatctg attttcttta acaccggcga agcggggttt
    18481 gtggagcttt ctgaccgcgt gacttccggt tcatcattaa tgccgcagaa gaaaaacccg
    18541 gatgcgctgg agctgattcg cggtaaatgc ggtcgggtgc agggtgcgtt aaccggcatg
    18601 atgatgacgc tgaaaggttt gccgctggct tacaacaaag atatgcagga agacaaagaa
    18661 ggtctgttcg acgcgctcga tacctggctg gactgcctgc atatggcggc gctggtgctg
    18721 gacggcattc aggtgaaacg tccacgttgc caggaagcgg ctcagcaggg ttacgccaac
    18781 gccaccgaac tggcggatta cctggtggcg aaaggcgtac ctttccgcga ggcgcaccat
    18841 atcgtgggtg aagcggtggt ggaagccatt cgtcagggca aaccgctgga agatctgccg
    18901 ctcgacgagt tgcagaaatt cagcccggtg attggcgaag atgtctatcc gattttgtcg
    18961 ctgcaatcgt gcctcgacaa gcgtgcggca aaaggcggcg tctcaccgca gcaggtggcc
    19021 caagcgattg cttttgcgca ggcgcggttg gggtaagaac atttatatgt acaaatttga
    19081 gcctggctta tcgccgggct tttttatggc aaaaaaaagc ggatcctgga gatccgcaaa
    19141 agttcacgtt ggctttagtt attcgagttg agaaactctc gaaacgggca gtgacttcaa
    19201 gggttaaaag aggtgccgct ccgtttctgt gagcaattat cagtcagaat gcttgatagg
    19261 gataatcgtt cattgctatt ctacctatcg ccatgaacta tcgtggcgat ggaggatgga
    19321 taatgaatat tcgtgatctt gagtacctgg tggcattggc tgaacaccgc cattttcggc
    19381 gtgcggcaga ttcctgccac gttagccagc cgacgcttag cgggcaaatt cgtaagctgg
    19441 aagatgagct gggcgtgatg ttgctggagc ggaccagccg taaagtgttg ttcacccagg
    19501 cgggaatgct gctggtggat caggcgcgta ccgtgctgcg tgaggtgaaa gtccttaaag
    19561 agatggcaag ccagcagggc gagacgatgt ccggaccgct gcatattggt ttgattccca
    19621 cggttggacc gtacctgcta ccgcatatta tcccgatgct gcaccagacc tttccaaagc
    19681 tggaaatgta tctgcatgaa gcacagaccc accagttact ggcgcaactg gacagtggca
    19741 aactcgattg cgtgatcctc gcgctggtga aagagagcga agcattcatt gaagtgccgt
    19801 tgtttgatga gccaatgttg ctggctatct atgaagatca cccgtgggcg aaccgcgaat
    19861 gcgtaccgat ggccgatctg gcaggggaaa aactgctgat gctggaagat ggtcactgtt
    19921 tgcgcgatca ggcaatgggt ttctgttttg aagccggggc ggatgaagat acacacttcc
    19981 gcgcgaccag cctggagacg ctgcgcaaca tggtggcggc aggtagcggg atcactttac
    20041 tgccagcgct ggctgtgccg ccggagcgca aacgcgatgg ggttgtttat ctgccgtgca
    20101 ttaagccgga accacgccgc actattggcc tggtttatcg tcctggctca ccgctgcgca
    20161 gccgctatga gcagctggca gaggccatcc gagcaagaat ggatggctat ttcgataaag
    20221 tgttaaaaca ggcggtttaa accgtttaac gcagctaccc gataggcttc cgccatggtc
    20281 gggtagttaa aggtggtgtt gacgaagtac tcaatagtgt tgccgccacc tttctgttcc
    20341 ataatcgcct gaccgatatg aataatttcg gcagcgcgct cgccaaagca gtgaataccc
    20401 agaatctctt ttgtttcccg atggaacaaa attttcagcg tgcccacgtt catgccgacg
    20461 atttgtgcgc gtgccagatg tttaaactgg gcgcggccca cttcatatgg cactttcatc
    20521 gcggtcagct gctgttcggt tttgcccaca gagctgattt ccgggatggt gtaaatgccg
    20581 gtagggatat cttcaatcag atgcgcggtg gcttcgcctt ttaccagcgc ctgcgcggca
    20641 atgcgcccct ggtcataggc cgctgatgcc aggctcggat aaccaatcac gtcgcccacc
    20701 gcgtaaacgt gcggctgtgc ggtctgatac atgctgttga ccttcagctg tccgcgactg
    20761 tcagtttcca gcccaatgtt ctgtaacgcc agcgaatcgg tattaccggt acgaccgttg
    20821 gcatagagca ggcagtcagc tttcagtttt ttacccgact tcagatgcat gatcacaccg
    20881 tcgtcacagc cttcgatctt ctcgtactct tcgttgtgac gaatcactac gccactgttc
    20941 cagaagtgat aggagagaga atctgacatc tcttgatcga gaaatgccag caggcgatcg
    21001 cgggtgttga tcagatccac ttttacatcc ataccgcgga agatcgacgc atattcacag
    21061 ccgatcactc cagcaccata gataagtaca tggcgcggtt cgtggtgcat gctaagaatt
    21121 gagtcgctgt cgtaaatgcg tggatgggtg aaatcaacat ctgttggatg atatggacga
    21181 gagccgcagg caataacaaa tttttcagcg gttagtgttt cgacgctgcc gtccgggcaa
    21241 tccagcgcca acgtatgctc gtcaacaaag cgagcgttcc cctgcaatat ttcacagtga
    21301 ttacgttcgt aaaatccctg acgcatgcgc gtttgttgat taatcacgtt atcggcatgg
    21361 ttaaggatat cggcaaaaga agagcggagc agtcgcgaat ggtcgctgta tagtgggttt
    21421 tgattgaatt ctataatgcg gctgacggcg tgacggagag ctttcgacgg gatggtgccc
    21481 cagtgggtgc aaccgccgcc aacattttga taacgctcga taactgcgac gcgcacacct
    21541 tgcttaacca ggcccattgc agcgccttcg ccgccggggc cggaacctat tactatggca
    21601 tcgtaatcgt aggaatgtgg catggtaggg cttacctgtt cttatacata aaagcaacag
    21661 aatggtaaca ttctatcgcg ggtaagccaa ttgatccccg tcatttatct ggctatatcc
    21721 tgagcggcct ttgctttgtc tgtttcttac ttttgccctg acgttttatt ggatttttat
    21781 cgacgatact ctccgtttaa gcggcaggtt tccgctgtac gtaaaagaac cggccagtaa
    21841 atatgtttta ttgcgttacc gttcattcac aatactggag caatccagta tgttcattct
    21901 ctggtatagt gccagcagta cttttggcaa ggattcagac atcgtgatgg gcataagagc
    21961 gcaacaaaaa gaaaaaaccc gccgttcgct ggtggaagcc gcatttagcc aattaagtgc
    22021 tgaacgcagc ttcgccagcc tgagtttgcg tgaagtggcg cgtgaagcgg gcattgctcc
    22081 cacctctttt tatcggcatt tccgcgacgt agacgaactg ggtctgacca tggttgatga
    22141 gagcggttta atgctacgcc aactcatgcg ccaggcgcgt cagcgtatcg ccaaaggcgg
    22201 gagtgtgatc cgcacctcgg tctccacatt tatggagttc atcggtaata atcctaacgc
    22261 cttccggtta ttattgcggg aacgctccgg cacctccgct gcgtttcgtg ccgccgttgc
    22321 gcgtgaaatt cagcacttca ttgcggaact tgcggactat ctggaactcg aaaaccatat
    22381 gccgcgtgcg tttactgaag cgcaagccga agcaatggtg acaattgtct tcagtgcggg
    22441 tgccgaggcg ttggacgtcg gcgtcgaaca acgtcggcaa ttagaagagc gactggtact
    22501 gcaactgcga atgatttcga aaggggctta ttactggtat cgccgtgaac aagagaaaac
    22561 cgcaattatt ccgggaaatg tgaaggacga gtaatgaaac aagcaaatca agatagaggt
    22621 acgctgctgc tggcgttagt tgctggctta tcgattaatg gtactttcgc agcgctgttt
    22681 agctccattg tgccattttc tgtattcccg attatttccc tggtgctgac ggtttactgc
    22741 ctgcatcaac gttatcttaa tcgcactatg ccggtaggct tgccgggtct ggcagctgcc
    22801 tgttttattc tcggcgtact gctgtacagc acggtagttc gtgcggaata tccggatatc
    22861 ggctctaact tcttcccggc agtactctcc gtcattatgg tgttctggat tggcgcgaag
    22921 atgcgtaacc gtaagcagga agttgctgag taatcggtat tatgccggat gctggcgcat
    22981 ccggcatggg ttttacttcg cggtcagtaa tacgccgcac tccatatggt gcgtgtaggg
    23041 gaactgatca aacagagcca gacgttcgac cttgtgcgtc tggcttaatg tttccagatt
    23101 cttgcataac gtttccgggt tacaggagat gtacaaaata cacggatacg cctgcaccat
    23161 tttctcggtt tcactgtcca gaccgctgcg cggtgggtcg acaaaaatgg tttcgcactg
    23221 ataactcttt aagtcgatcc cttgcaggcg gttaaactcg cgcacaccat tcatcgcctg
    23281 agtaaattct tctgccgcca tacgaataat ttgtacgtta tcaatatggt tagctgcgat
    23341 gttgtattgc gcagcagcaa ccgacggctt agcgatttcg gtggctaata cccgatcaaa
    23401 attgcgcgcc agcgctaatg aaaagttacc gttgccgcag tacagctcca gtaaatcgcc
    23461 ttttgagcct ttggttacgt ccagcgccca ttccagcatc tgaatattca tcgccgcgtt
    23521 cggctgggta aagctgtttt ctacctgacg gtagatcatc tctttccctg cgaccggcag
    23581 acgctcgtcg atgtaatctt gatccagctc gattttggtt ttcgttgccc gaccaatcag
    23641 atgcacattc agattctgcg cgcgcagtgc atcgcgcagg gcctccgctt cctgacgcca
    23701 ctcatcatcc agcttcttat ggtatagcag ggaaaccacc gcctgattac tcagcgtggt
    23761 gaggtaatca atctggaaca acttgtggcg cagaacggga ttattacgca cacccgcaat
    23821 catcgccgtc atcaactggt tgataagttc actggcggcg gggaagctat ccacgcggat
    23881 acggcttttg gtttgttgat cgaaaatgat gtgatacagg tcatcgccat cgtgccagat
    23941 gcggaactcc gcacgcatcc ggtaatgact gaccggcgag cgaaacactt ccggaaccag
    24001 gtcagaaaac ggtgccatca tactttgcaa acgtaccact ttttcggcta actgcgcttc
    24061 atactgttct gttggaaggt gttcgggggt catgatgtat cctgaaaaat taaagtacgc
    24121 ggcgattgta gggattgctc atcagatgtc cagatcttga tgaattccta tttgtgagct
    24181 acgtctggac agtaacttgt tacaacctgt agcatccact tgtcggtcct gtgagttaat
    24241 agggaatcca gtgcgaatct ggagctgacg cgcagcggta aggaaaggtg cgatgattgc
    24301 gttatgcgga cactgccatc cggtgggaag tcatcatctc ttagtatctt agatacccct
    24361 ccaagcccga agacctgccg gccaacgtcg catctggttc tcatcatcgc gtaatattga
    24421 tgaaacctgc ggcatccttc ttctattgtg gatgctttac aatgattaaa aaagcttcgc
    24481 tgctgacggc gtgttctgtc acagcctttt ccgcttgggc acaggatacc agcccggata
    24541 ctctcgtcgt tactgctaac cgttttgaac agccgcgcag cactgtgctt gcaccaacca
    24601 ccgttgtgac ccgtcaggat atcgaccgct ggcagtcgac ctcggtcaat gatgtgctgc
    24661 gccgtcttcc gggcgtcgat atcacccaaa acggcggttc aggtcagctc tcatctattt
    24721 ttattcgcgg tacaaatgcc agtcatgtgt tggtgttaat tgatggcgta cgcctgaatc
    24781 tggcgggggt gagtggttct gccgacctta gccagttccc tattgcgctt gtccagcgtg
    24841 ttgaatatat ccgtgggccg cgctccgctg tttatggttc cgatgcaata ggcggggtgg
    24901 tgaatatcat cacgacgcgc gatgaacccg gaacggaaat ttcagcaggg tggggaagca
    24961 atagttatca gaactatgat gtctctacgc agcaacaact gggggataag acacgggtaa
    25021 cgctgttggg cgattatgcc catactcatg gttatgatgt tgttgcctat ggtaatacag
    25081 gaacgcaagc gcagacagat aacgatggtt ttttaagtaa aacgctttat ggcgcgctgg
    25141 agcataactt tactgatgcc tggagcggct ttgtgcgcgg ctatggctat gataaccgta
    25201 ccaattatga cgcgtattat tctcccggtt caccgttgct cgatacccgt aaactctata
    25261 gccaaagttg ggacgccggg ctgcgctata acggcgaact gattaaatca caactcatta
    25321 ccagctatag ccatagcaaa gattacaact acgatcccca ttatggtcgt tatgattcgt
    25381 cggcgacgct cgatgagatg aagcaataca ccgtccagtg ggcaaacaat gtcatcgttg
    25441 gtcacggtag tattggtgcg ggtgtcgact ggcagaaaca gactacgacg ccgggtacag
    25501 gttatgttga ggatggatat gatcaacgta ataccggcat ctatctgacc gggctgcaac
    25561 aagtcggcga ttttaccttt gaaggcgcag cacgcagtga cgataactca cagtttggtc
    25621 gtcatggaac ctggcaaacc agcgccggtt gggaattcat cgaaggttat cgcttcattg
    25681 cttcctacgg gacatcttat aaggcaccaa atctggggca actgtatggc ttctacggaa
    25741 atccgaatct ggacccggag aaaagcaaac agtgggaagg cgcgtttgaa ggcttaaccg
    25801 ctggggtgaa ctggcgtatt tccggatatc gtaacgatgt cagtgacttg atcgattatg
    25861 atgatcacac cctgaaatat tacaacgaag ggaaagcgcg gattaagggc gtcgaggcga
    25921 ccgccaattt tgataccgga ccactgacgc atactgtgag ttatgattat gtcgatgcgc
    25981 gcaatgcgat taccgacacg ccgttgttac gccgtgctaa acagcaggtg aaataccagc
    26041 tcgactggca gttgtatgac ttcgactggg gtattactta tcagtattta ggcactcgct
    26101 atgataagga ttactcatct tatccttatc aaaccgttaa aatgggcggt gtgagcttgt
    26161 gggatcttgc ggttgcgtat ccggtcacct ctcacctgac agttcgtggt aaaatagcca
    26221 acctgttcga caaagattat gagacagtct atggctacca aactgcagga cgggaataca
    26281 ccttgtctgg cagctacacc ttctgaacca cgtcccaccg tgctggtgtt tgactccggc
    26341 gtcggtgggt tgtcggtcta tgacgagatc cggcatctct taccggatct ccattacatt
    26401 tatgctttcg ataacgtcgc tttcccgtat ggcgaaaaaa gcgaagcgtt tattgttgag
    26461 cgagtggtgg caattgtcac cgcggtgcaa gaacgttatc cccttgcgct ggctgtggtc
    26521 gcttgcaaca ctgccagtac cgtttcactt cctgcattac gcgaaaagtt cgacttcccg
    26581 gttgttggtg tcgtgccggc gattaaacct gctgcacgtc tgacggcaaa tggcattgtc
    26641 ggattactgg caacccgcgg aacagttaaa cgttcttata ctcatgagct gatcgcgcgt
    26701 ttcgctaatg aatgccagat agaaatgctg ggctcggcag agatggttga gttggctgaa
    26761 gcgaagctac atggcgaaga tgtttctctg gatgcactaa aacgtatcct acgcccgtgg
    26821 ttaagaatga aagagccgcc agataccgtt gtattgggtt gcacccattt ccctctacta
    26881 caagaagaac tgttacaagt gctgccagag ggaacccggc tggtggattc tggcgcagcg
    26941 attgctcgcc gaacggcctg gttgttagaa catgaagccc cggatgcaaa atctgccgat
    27001 gcgaatattg ccttttgtat ggcaatgacg ccagaagctg aacaattatt gcccgtttta
    27061 cagcgttacg gcttcgaaac gctcgaaaaa ctggcagttt taggctgatt tggttgaatg
//
LOCUS       DAKISA010000051        26801 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.051, whole genome shotgun sequence.
ACCESSION   DAKISA010000051 DAKISA010000000
VERSION     DAKISA010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26801
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.051"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            140..475
                     /locus_tag="OVW45_002271"
     CDS             140..475
                     /locus_tag="OVW45_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCV5526702.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQP
                     AKPAAQPAA"
     gene            568..651
                     /locus_tag="OVW45_002272"
     CDS             568..651
                     /locus_tag="OVW45_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5526703.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            751..1572
                     /locus_tag="OVW45_002273"
     CDS             751..1572
                     /locus_tag="OVW45_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCV5526704.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTNDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1611..1940)
                     /gene="mdtI"
                     /locus_tag="OVW45_002274"
     CDS             complement(1611..1940)
                     /gene="mdtI"
                     /locus_tag="OVW45_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCV5526705.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1927..2292)
                     /gene="mdtJ"
                     /locus_tag="OVW45_002275"
     CDS             complement(1927..2292)
                     /gene="mdtJ"
                     /locus_tag="OVW45_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCV5526706.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2704..3738
                     /gene="tqsA"
                     /locus_tag="OVW45_002276"
     CDS             2704..3738
                     /gene="tqsA"
                     /locus_tag="OVW45_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCV5526707.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3763..5151)
                     /gene="pntB"
                     /locus_tag="OVW45_002277"
     CDS             complement(3763..5151)
                     /gene="pntB"
                     /locus_tag="OVW45_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCV5526708.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5162..6694)
                     /gene="pntA"
                     /locus_tag="OVW45_002278"
     CDS             complement(5162..6694)
                     /gene="pntA"
                     /locus_tag="OVW45_002278"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCV5526709.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7218..8162
                     /locus_tag="OVW45_002279"
     CDS             7218..8162
                     /locus_tag="OVW45_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCV5526710.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEILGVATTA
                     SPSSEVGRFFETQSSKGGRYTVMLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            8348..9730
                     /locus_tag="OVW45_002280"
     CDS             8348..9730
                     /locus_tag="OVW45_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCV5526711.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9767..10489
                     /gene="folM"
                     /locus_tag="OVW45_002281"
     CDS             9767..10489
                     /gene="folM"
                     /locus_tag="OVW45_002281"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCV5526712.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSPHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10486..10821)
                     /locus_tag="OVW45_002282"
     CDS             complement(10486..10821)
                     /locus_tag="OVW45_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCV5526713.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVSCWGISAWV
                     LIICWIKLH"
     gene            10950..11669
                     /gene="rstA"
                     /locus_tag="OVW45_002283"
     CDS             10950..11669
                     /gene="rstA"
                     /locus_tag="OVW45_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCV5526714.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            11673..12974
                     /gene="rstB"
                     /locus_tag="OVW45_002284"
     CDS             11673..12974
                     /gene="rstB"
                     /locus_tag="OVW45_002284"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCV5526715.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALITFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            13050..13979
                     /gene="tus"
                     /locus_tag="OVW45_002285"
     CDS             13050..13979
                     /gene="tus"
                     /locus_tag="OVW45_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCV5526716.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(13976..15379)
                     /gene="fumC"
                     /locus_tag="OVW45_002286"
     CDS             complement(13976..15379)
                     /gene="fumC"
                     /locus_tag="OVW45_002286"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCV5526717.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQVKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLVSGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(15522..17168)
                     /gene="fumB"
                     /locus_tag="OVW45_002287"
     CDS             complement(15522..17168)
                     /gene="fumB"
                     /locus_tag="OVW45_002287"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCV5526718.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            17367..18542
                     /gene="manA"
                     /locus_tag="OVW45_002288"
     CDS             17367..18542
                     /gene="manA"
                     /locus_tag="OVW45_002288"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCV5526719.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            18643..20151
                     /locus_tag="OVW45_002289"
     CDS             18643..20151
                     /locus_tag="OVW45_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCV5526720.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(20332..21642)
                     /gene="uidC"
                     /locus_tag="OVW45_002290"
     CDS             complement(20332..21642)
                     /gene="uidC"
                     /locus_tag="OVW45_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCV5526721.1"
                     /translation="MRKIVAMAVICLTAASGLISAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKSGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAQIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLKSEITPERKTSGVSDC"
     gene            complement(21681..23054)
                     /gene="uidB"
                     /locus_tag="OVW45_002291"
     CDS             complement(21681..23054)
                     /gene="uidB"
                     /locus_tag="OVW45_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HCV5526722.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGVLFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSRKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(23051..24862)
                     /gene="uidA"
                     /locus_tag="OVW45_002292"
     CDS             complement(23051..24862)
                     /gene="uidA"
                     /locus_tag="OVW45_002292"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCV5526723.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(25251..25841)
                     /gene="uidR"
                     /locus_tag="OVW45_002293"
     CDS             complement(25251..25841)
                     /gene="uidR"
                     /locus_tag="OVW45_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCV5526724.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIKGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(26062..>26801)
                     /gene="hdhA"
                     /locus_tag="OVW45_002294"
     CDS             complement(26062..>26801)
                     /gene="hdhA"
                     /locus_tag="OVW45_002294"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HCV5526725.1"
                     /translation="GKCAIITGAGAGIGKEIAITFATAGASVVVSDINADAANHVVDE
                     IQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPKPFDMPMADFR
                     RAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYASSKAAASHLV
                     RNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRLGQPQDIANAA
                     LFLCSPAASWVSGQILTVSGGGVQELN"
BASE COUNT         6728 a         6937 c         6440 g         6696 t
ORIGIN      
        1 ggttatcgct cgccacggga atatctgcgg cagcgggctt gtaatgggtt aagtgataac
       61 agatgtctgg aaatataggg gcaaatccac tgccattccc agggatatag ttatttcaac
      121 ggccccgcgg tggggttaaa tgaaaaaaca aattgagggt atgacaatga aaaaagtatt
      181 agctctggtt gttgccgctg ctatgggtct gtcttctgcc gcctttgctg cagagactgc
      241 gaccacacct gctccgactg cgacgaccac caaagcagcg ccggcgaaaa cgacacatca
      301 taaaaaacag cataaagcag cacctgccca gaaagcgcag gcggctaaaa agcatcataa
      361 aaatacgaaa gctgaacaga aagcccctga acaaaaagcg caggcagcga agaaacacgc
      421 cgggaaacac agccatcagc aaccggcaaa acctgctgca caacccgcag cgtaagtttt
      481 caacagtaat gctggcgcgc cccctcgcgc ctgaaaatta cggtgctaag cgggtaacgt
      541 ttagcaccgc ctttagcgga gggtagtatg gtgggccgtt atcgctttga gttcattctt
      601 atcatcctta ttttatgcgc actgattgcc gcccgttttt atctttcctg actgtagtta
      661 tctgatttta ctcccacttt catcccgtcc cgtctatagt atttatgagg gtttgctttt
      721 aataatcata attacccacc agagtgtgat atgcgtacaa ccattgctgt agtgttgggt
      781 gcaattagtt tgacgtctgc ttttgtgttt gcagataaac cagacgttgc caaatcggca
      841 aacgatgagg tcagcaccct gttttttggt catgatgatc gtgtgccagt gaatgacacg
      901 acccaatcac cgtgggatgc ggttgggcaa ctggaaacgg ccagcggcaa tttatgtacg
      961 gcgacgctga ttgcacccaa tctggcatta acggcaggac actgtttatt gacacctcca
     1021 aagggtaaag ccgataaagc agtggcgctg cgttttgtgt caaataaagg tctttggcgc
     1081 tatgagatcc acgacataga aggccgcgtt gatccgacac tgggaaagcg gttaaaagca
     1141 gatggggatg gttggattgt acctcccgca gccgcgccgt gggacttcgg attgattgtg
     1201 ctacgtaatc ccccttctgg cattacaccg ttgccgttat ttgagggaga taaagccgcg
     1261 cttactgccg cattaaaatc ggctggccgt aaagtaactc aggcgggata ccctgaagat
     1321 catctcgata cgttgtacag tcatcaaaac tgtgaagtga ctggctgggc gcaaacgtcg
     1381 gtgatgtcac atcagtgcga taccttgccg ggtgacagcg gttcgcctct gatgttgcat
     1441 accaatgacg gctggcaatt aattggggtg caaagttcgg ctcctgccgc gaaagatcgc
     1501 tggcgcgccg ataaccgggc catttctgtt accggttttc gcgacaagct ggatcaactg
     1561 tcgcaaaaat aatgttcaaa cgctgcccga ctgtgcgggc agcgtgttca tcaggcaagt
     1621 ttcaccatga tcattccagc caacagcaag accaggccaa tccagccttt acgatttaac
     1681 cgttgaccaa acaagatcca acctgcggct aacgtggcgg caataccaaa cccgccccac
     1741 aatgcataag cgacagacaa gtcgatccct ttaacggcct gagaaagcgc actaaaggca
     1801 gccagcaccg ccgccaggga gagcaagcca aatattttgc gacgaaagcc gtcagaaaat
     1861 ttcaaaaaga cgttagcaac gatttccagc acgattgcca atgccagcca ggcggcgtga
     1921 acccattcaa actgcgccat ggttcacctc cagttcaggt ttacgcgctt tacgggtacc
     1981 tgattttatc aacacaatcc cggcgaccag ggtggttaac ccggcaattt tcatcagcga
     2041 taaactttcg tcgaataaca aaacgctaaa caaggtaata aataaaatac cgataccttc
     2101 ccacagcgca taagctacgc ctaaggcgat ctttttaacg gcgaaagaga gaaatatata
     2161 cgacagagaa atcatcacca gcattaaaat aaagccgcca tttccctcac tgacgctcgc
     2221 ccatttcatt gacagcgtac cggtaatttc tgtagcaata gccaggccta ataaaatcca
     2281 ataaatatac attgtccttc tcctgcaaga gaattatttt aattttcgct taattcagcg
     2341 aaaccaaagt aaagtggtca agccccgcgc atagggcaag cttaggcaga agaaaggact
     2401 aaagcgcgtt gcgccagtgc tgctcaccta cgagcaagat agtggatgag gtacgaagat
     2461 gggtaaatgt tgaaaataac gtcctgaaca aattgtccat aatattacaa ttatccgcag
     2521 tgttgcttct cgtcatcgcg gatgataatt gtcctcggta gttgaacacg cctgatttgt
     2581 atcatagctt aagaattaac tcaaaatatt ttcacttctt tacctgagcg gtttgatttt
     2641 cgttatgatg acggagcgaa aaagacatta ttattagcaa aggaagaaaa aacggggaca
     2701 agcatggcaa agccgatcat cacgctcaat ggcctaaaaa tcgtcattat gttgggaatg
     2761 ctggtcatta ttctctgcgg tatccgtttt gccgccgaga tcatcgtgcc gtttattctc
     2821 gcattattta ttgctgttat tcttaacccg ctggtgcaac acatggtccg ctggcgagtg
     2881 ccgcgtgtac tggcggtgtc gattttgatg accatcatcg tgatggcgat ggtgttgcta
     2941 ttagcttatc tgggttccgc gctcaacgag ttgacgcgga cgttaccgca atatcgcaac
     3001 tctattatga cgccgctgca agctcttgag ccgttgttgc aacgcgtagg gattgacgtc
     3061 tcagttgacc agctggcgca ttacattgat ccgaacgcgg cgatgacgtt gctcaccaac
     3121 ttattgacgc agttatctaa tgccatgtca tcaatatttt tattgctgct gacggtgctg
     3181 tttatgctgc tcgaagtgcc acaattgccc ggaaaatttc agcaaatgat ggcgcgtccg
     3241 gttgaaggga tggcggcgat tcaacgtgca attgacagtg tttctcatta tctggtgctg
     3301 aaaacagcca tcagcatcat caccggcctg gtcgcctggg cgatgctcgc cgcactcgat
     3361 gttcgcttcg cttttgtctg gggattgctg gcctttgcgc ttaattacat ccctaatatt
     3421 ggttcagttc tcgcggcaat cccccctatc gctcaggtac tggtgtttaa tggcttctac
     3481 gaagcgttgc tggtgctggc gggatatctg ctgattaatc tggtcttcgg caatattctg
     3541 gagccgcgca tcatgggacg tgggctgggg ctttccacat tggtggtatt tttgtcgttg
     3601 attttttggg gatggttgtt aggaccggtg ggtatgctgc tttccgtgcc gttaacaatt
     3661 attgtcaaaa ttgcgcttga acaaacagcg ggaggtcaaa gcatcgccgt tctgttaagc
     3721 gatctcaata aagagtgacg gcctccgcag aggccgtcag ggttacagag ctttcaggat
     3781 tgcatccacg ctggctttgg cgtcaccaaa cagcatgtgg gtgttttcct tgaagaacag
     3841 cgggttttgc acaccagcat agccagtgtt catcgaacgt ttaaagacaa tcacgttctg
     3901 cgctttccac acttccagca caggcatacc agcaatagga ctcttcggat catcctgcgc
     3961 cgccgggtta accgtatcgt tagcaccaat caccagtacg gtatcggtat cagcaaagtc
     4021 atcgttgatc tcgtccattt ccagcacgat gtcatacggt acttttgctt cagccagcaa
     4081 tacgttcata tgtccaggca aacgccccgc aaccgggtgg ataccgaaac gcacgttgat
     4141 accacgagcg cgcagtttct cggtaatttc agcgacagga tattgcgcct gcgcgactgc
     4201 catgccgtac cccggagtaa tgatcactga atgggagttt ttcagcagtt ccgctgtctc
     4261 ttctgcggtg atttcgcggt gctcgcccac ttcctgatca tcgccagtag aagagccgtc
     4321 ggtgccgaaa ccacccgcaa taacgctgat aaaggaacgg ttcatcgcct tacacataat
     4381 gtaagaaagg atagcccccg aagaaccgac cagcgcaccg gtcacaatca gcaggtcgtt
     4441 gctgagcata aagcccgcag ccgcagccgc ccagccggag tacgagttca gcatcgacac
     4501 caccactggc atatctgcac caccgatgga ggcgactaaa tgccagccga ataccagcgc
     4561 aatggcggtc attattagca atgccagcac ttgcaggccg acgctgtccg tgcgaacaaa
     4621 tacaatcagc agcaggaagg aaacgaccag agccgccagg ttcattttgt gacggtttgg
     4681 cagcatcaac ggtttagacg aaatcttgcc acacagtttg ccgaacgcca ccaccgaacc
     4741 cgtgaacgtt accgccccga tgaagatacc gaggaacact tccgtcaggt gaatattgac
     4801 cagaatcggt gccattcccg cgtcatgatg cagatagctg ttaaagccaa ccagcactgc
     4861 cgccagaccc acgaagctgt gcaggatcgc caccagttct ggcatttcgg tcatttcaac
     4921 tttcttcgcc agacggatac caattgcccc accaatgacc atcgccagca agatccagcc
     4981 aacattaccc gtatccggtc caaaaatggt tgcgattaac gcaatcgcca tcccggcgat
     5041 accgaagttg ttaccctggc gagacgtttc atgtttcgaa agaccggcca gactgaagat
     5101 aaacaggatc gcggcaacaa tgtatgcagc tgtaactaat cctccagaca tatgttaccc
     5161 cttaattttt gcggaacatt ttcagcatgc gctgagtcac ggtgaagcca ccgaaaatat
     5221 taatgctggc tataagcacc gcgataaaac taaggaagct aacccagccg ccctggccaa
     5281 tctgcaacag tgctccgaca acaataatcc ctgaaatcgc gttggtgacc gacatcaacg
     5341 gtgtatgcag cgcgtgcgat acattccaca ccacgtaata accgacaacg caggccagcg
     5401 cgaaaacggt gaagtgccca agaaactctt tcggcgcaac gcttgccagc caaccgaaaa
     5461 ggatgattgc cagcgccatc aacgcgtatt tacgccacgg tgagcaggca catttttcct
     5521 cagttttcac ttccggtgcc gctttttgtg ccgcctgcgg ctgagctgat acctgaatcg
     5581 gcggtgccgg ccaggtaatt tcgcccgcac ggatcacggt cacgccgcga atcaccacat
     5641 catcaaaatc aacagtgata ttgccgtctt tctctttgca caacagtttc agcagattaa
     5701 cgaggttagt accgtaaagc tgcgaggatt gcgtcggcag acggcccgga agatcggtat
     5761 aaccaatcac tttgacacca ttttccgtag tgaagatttc acccggcacg gtgtattcac
     5821 agttgccacc gttttgggct gccaggtcga caatcacact gcccgccttc atggagtcaa
     5881 ccatttcacg ggtaattagc ttcggcgctg gtttgcctgg aataagcgcg gtggtgacaa
     5941 tgatatcgac ctcttttgcc tgggcggcaa agagttccat ttccgcttta atgaacgcgt
     6001 ccgacatcac tttggcatag ccatcgccgc tgcccgcttc ctctttaaaa tccagctcga
     6061 ggaattccgc gcccatactt tgaacttgtt ctttcacttc cggacgggtg tcgaatgcac
     6121 gcacaatcgc gccgagactg tttgctgcgc caatggcggc cagacctgca acacccgcac
     6181 caatcaccat cacttttgcc ggtggcactt tcccggccgc agtaatttgc ccggtaaaga
     6241 agcgcccaaa ttcatgtgcc gcttcaacaa tggcgcgata accggcgatg ttcgccatcg
     6301 agcttagtgc gtccagcgat tgtgcgcgtg agatacgcgg cacagaatcc atcgccatca
     6361 cggtcacgtt acgttccgca agtttttgca ttaattccgg attctgcgca ggccagataa
     6421 aactcaccag cgttgtccca ggattcagta acgcaatttc atcatctaac ggcgcattga
     6481 ccttcagaat gatctctgac tgccagacgc tattcccttc tacaatttca gcgcccgctt
     6541 gcacaaacgc tttatcgtca aaacttgcca gttgacccgc gccgctctct accgcgacgg
     6601 taaaacccag tttcagcagc tgttccactg tttttggcgt tgctgcaaca cgggtttcat
     6661 tggttaaccg ttctcttggt atgccaattc gcatgatatt cccttccatc ggttttattg
     6721 atgatggttt gcctgtgtca ggagccacac aagccgctca tgtacgagct aaatgttact
     6781 ccgttaaaat aaattagtaa caaacgccct ataacgtact gaaaattatg cctgtgatct
     6841 agcgccaaaa atcagtattt cggcgtgaac tcgcaaaata ttaacgattc agccgtgata
     6901 gtgggataaa caccttagaa cgccggataa agactgataa ttgtcttcga cggtcgggta
     6961 aaacgagaca tcgccccggc acgattcact acttaacatt aaattaactt atacaattca
     7021 gttgcttcag tagtaatgat gctgatacgg ctgtttttta agcatagacg gtcatttgag
     7081 caggattaaa attggcttaa ggaatgtgat atgaaaaatg acgcagacag ttacaccgtt
     7141 taaatgcaat aatcagccac gtttctcgtt aataacaata ccagtacctg gtttgcgcaa
     7201 ggcgaaggat tatttttatg aagcttaaga acaccctcct ggcgtcggca ctgctttctg
     7261 ctacggcatt ctccgttaac gcagcaacag aactgacacc ggagcaagcg gcagcggtta
     7321 aaccttttga ccgtgtagtg gttaccggtc gttttaatgc tattggcgaa gcggtgaaag
     7381 ccgtttctcg tcgcgcagat aaagaaggtg ccgcctcttt ttatgttgtc gacacttctg
     7441 attttggtaa cagcggtaac tggcgtgtgg tcgctgacct ctataaagcc gatgctgaaa
     7501 aagcagaaga aacaagtaat cgcgtaatta acggtgttgt cgaactgccg aaagatcagg
     7561 ctgttctgat tgaaccgttt gacacggtca ccgtccaggg cttctatcgt agccagccag
     7621 aagtcaatga tgccatcacc aaagcggcaa aagcgaaagg tgcctactct ttctacatcg
     7681 ttcgtcaaat cgatgccaac caaggcggca accagcgtat tactgcattc atctataaaa
     7741 aagatgctaa gaaacgtatc gtccagagcc cggatgtgat cccggcagat tccgaagcag
     7801 gacgtgcagc tctggctgcc ggtggcgaag ccgcgaagaa agttgagatc ctaggtgttg
     7861 cgactaccgc atcaccaagt tctgaagtcg gtcgtttctt tgaaacccag tcatcaaaag
     7921 gcgggcgtta caccgtcatg ctcccggatg gcactaaagt cgaagaactg aacaaagcga
     7981 ccgcagcgat gatggtcccg ttcgacagca tcaaattctc tggcaactac ggcaacatga
     8041 ccgaagtctc ctatcaggtt gcgaaacgtg ccgcgaagaa aggtgctaag tattaccaca
     8101 tcacccgcca gtggcaggaa cgtggtaata acctgaccgt cagcgcagat ctgtataaat
     8161 aactattcag ttactcctat aaggcggctt gatgccgcct ttcgcatttt tagtcacaaa
     8221 tttccccgcg accattgcat gcagacacat ctcttcgtaa aatcccgcgc ttcagtggca
     8281 aactccattt ttatgcgttt tgccctaata tttattcttc ttatcacgtt ttaatcactg
     8341 gatatcgatg gaaaagaaac tgggactgag cgcactcacc gcgctggtat taagctcaat
     8401 gctgggcgcg ggtgttttca gtctgccgca aaatatggcg gcagttgcca gcccggcagc
     8461 actgctcatc ggctggggta ttactggcgc tggcatttta ttgctggcct ttgccatgct
     8521 gatcctcacg cgcattcgtc ccgaacttga cggcggtatc tttacctatg cccgcgaagg
     8581 gtttggcgag ctaatcggct tttgttccgc atggggatac tggctgtgcg cagtcatcgc
     8641 caacgtctcc tatctggtga tcgttttttc cgcgttaagc ttttttacgg acacgccgga
     8701 attgcgcctg ttcggcgatg gtaatacctg gcaatcgatc gtcggtgcat cggctttatt
     8761 gtggattgtt cactttttga tcctgcgcgg ggtgcaaacc gctgccagca ttaacctggt
     8821 ggcgacactg gcaaaattgt tgccgctggg tctgtttgtt gtgctggcga tgatgatgtt
     8881 caaactggat accttcaagc tcgacttcac cggacttgca cttggcgtac ccgtttggga
     8941 acaggtgaaa aacaccatgc tgatcaccct gtgggttttc attggtgtgg aaggtgccgt
     9001 ggttgtttct gcgcgtgcgc gtaataaacg tgatgttggc aaagcgacac tgctggcggt
     9061 tctctccgct ctgggcgttt acttgctggt aacgctgctt tcgctgggtg tggtggcacg
     9121 tcctgaactg gctgaaattc gtaacccgtc aatggccggt ctgatggtgg aaatgatggg
     9181 accatggggc gaaatcatca tcgctgccgg tttgatcgtt tccgtttgcg gcgcgtactt
     9241 aagctggacc atcatggcgg cagaagttcc gttcctggct gccactcata aagcattccc
     9301 acgcattttt gcgcgccaga acgcacaagc tgcgccatcc gcctcactgt ggttgaccaa
     9361 catctgcgtg caaatttgtc tggtactcat ctggctgacc ggttccgatt acaacacgtt
     9421 gctgaccatc gcctcagaaa tgattctggt gccctatttc ctggtcggcg cgttcttgct
     9481 gaaaatcgca acccgtccac tgcataaagc ggtaggtgtc ggtgcctgca tttatggctt
     9541 atggttattg tacgcctctg gtccaatgca cttgctgtta tccgttgttc tgtatgcgcc
     9601 ggggctgctg gttttcctgt atgcgcgcaa aacgcataca catgataatg tactgaaccg
     9661 tcaggaaatg gtgctgattg gcatgctgtt aattgcctca gttccggcga cctggatgct
     9721 ggtgggataa cgttcatccc atcgttggat agcaaaggag ataacgatgg gtaaagccca
     9781 gcccttgcca atattaatta ctggcggagg tcgtcgcatc ggcctcgccc tcgcctggca
     9841 tttcattaat caaaagcaac cggtgattgt cagctatcgg acgcactatc cagccattga
     9901 tggactgatt aaggcaggtg cgcagtgtat tcaggctgat ttttcgacca acgacggtgt
     9961 gatggcgttt gccgatgaag tactaaaaag cccccatggt ctgcgtgcta ttttgcataa
    10021 cgccagtgcg tggatggcgg aaaaaccggg tgcgccactg gccgacgtac tggcttgcat
    10081 gatgcagatc cacgttaata ccccatacct gctcaaccat gcgctggaaa gattactgcg
    10141 tgggcacgga cacgccgcca gcgatatcat tcactttacc gattatgtgg tggagcgcgg
    10201 tagcgacaag catattgcgt atgctgcaag caaagcggca ctggataata tgacccgctc
    10261 gtttgcccgc aagctggcac cggaagtgaa agtgaactct attgctccat cgctgatcct
    10321 gtttaatgaa catgatgatg ccgaatatcg acaacaggcg ctgaataaat cactgatgaa
    10381 aaccgcgcct ggcgagaaag aagtgatcga cctggtcgat tacttactta ccagttgctt
    10441 tgtcaccgga cgcagtttcc cacttgatgg cggtcgtcat ctgcgttaat gcagctttat
    10501 ccagcatata atcaacaccc atgcgcttat cccccaacac gaaacagaac caacaaaagc
    10561 ggcgggcaag cgcatcattc cggtgaaata ccacagcgac accaggtaaa caaaataggg
    10621 aataatcgac cacatactaa aaatgatggt tgcgcgtaag gcttcaatgc cgcgttcgct
    10681 ggcaacaata taatgcgcga taagcgcaaa ggtcgggaaa agtggaatca gcccggcgat
    10741 ataataattt ttcgtttttg ctaaaacacc aatcaacagc actaccagcg caccgagcgc
    10801 ggctttgatt accagcccca tttttttacc ttaacacttc cataacaagt catcagtaga
    10861 atacctgatg aaaacttgtt tagaaacgat tgatagtaag taaaaacagc gcggtgtatt
    10921 gtgacgtttt tatatctacc gtgaatgtta tgaacactat cgtatttgtg gaagatgatg
    10981 cggaagtcgg ttcactgatt gccgcgtacc tggcaaaaca tgatatgcag gttaccgtag
    11041 agccgcgcgg cgaccaggcc gaagaaacca ttttgcgaga aaatccggat ttggtgttac
    11101 tcgacatcat gctaccaggc aaggacggca tgaccatttg tcgtgattta cgcgcaaagt
    11161 ggtctggacc gattgttctt ctaacctctc tcgatagcga tatgaaccac atcctggcac
    11221 tggaaatggg tgcctgcgac tatattctca aaacgacgcc ccctgctgtt ttgctagcgc
    11281 gtttacgttt gcatttgcgt cagaatgagc aagccacact gaccaaaggt cttcaggaaa
    11341 cgtctctgac tccctacaaa gccctgcatt tcggcacgtt gaccatcgat cccatcaacc
    11401 gcgtagtcac cctggctaac actgaaatct cgctctcgac agctgatttc gaattattgt
    11461 gggaattagc tacccatgcc gggcaaatca tggaccgcga tgcattgctg aaaaatttac
    11521 gcggcgtcag ttatgacgga ctggatcgta gcgtggacgt ggctatttcg cggttaagaa
    11581 aaaaactgct cgataacgcc gcagaacctt atcgcattaa aactgtgcgt aacaaaggct
    11641 atctttttgc gcctcatgca tgggaataag cgatgaaaaa actgtttatc cagttttacc
    11701 tgttattgtt tgtctgcttc cttgtgatgt ctctgctggt tgggctggtg tacaaattta
    11761 ccgccgaacg cgcgggcaaa cagtcgctgg atgatttgat gaacagttca ctgtatctga
    11821 tgcgcagcga attgcgtgag atccccccac acgactgggg taagacgctg aaagagatgg
    11881 atttaaatct ctctttcgat ctgcgtgtcg agccactgag taaataccat cttgatgata
    11941 tttccatgca ccgactgcgt ggcggcgaaa ttgtcgccct ggacgatcag tacacgtttt
    12001 tgcagcgtat cccgcgcagc cactacgtgc tggcagttgg tcctgttcct tatctttatt
    12061 atctccatca gatgcgattg ctggatatcg ccctgatcac ttttattgct atttccctcg
    12121 cctttccggt gtttatctgg atgcgtccgc actggcagga tatgttaaaa ctggaagcag
    12181 cggcgcaacg atttggcgat gggcatctca atgaacgtat ccactttgat gagggttcga
    12241 gctttgaacg acttggtatc gcatttaacc agatggcgga caatatcaac gccttgattg
    12301 ccagcaaaaa acagcttatt gacggtatcg ctcacgaact gcgaacaccg ttagtgcgcc
    12361 tgcgttatcg actggagatg agcgataacc tgagcgccgc cgaatcccag gcgttgaatc
    12421 gtgatatcag tcaacttgaa gctttaattg aagagctgct gacttatgcc cgactcgatc
    12481 gcccacaaaa cgagcttcat cttagcgaac cagacctgcc gttgtggctg tcaacgcatc
    12541 tggcagacat tcaggcagta acgcccgata aaacggtacg gattaaaacg ctcgcgcaag
    12601 gccattatgc ggcgttggat atgcgcttaa tggagcgcgt gctggataat ttgctcaata
    12661 acgccctgcg gtactgccat tcaacggttg aaaccagcct gctactgtcg gggaatagag
    12721 cgacattaat tgttgaggat gatggcccag ggattgcccc agaaaaccgc gaacatatct
    12781 ttgaaccttt tgttcgcctc gatcccagcc gggatcgctc aaccggcggc tgcgggctgg
    12841 ggctggcaat tgtccactct atagcactgg caatgggcgg tacggttaat tgtgacacca
    12901 gcgaactggg tggtgctcgc ttctcgttta gctggccgtt atggcataac atcccgcaat
    12961 ttacctctgc ctgacactac gcgcacgatg gtcaagtcac caccactgtg ctataaaata
    13021 agtatgttgt aactaaagtg gttaatatta tggcgcgtta cgatctcgta gaccgactca
    13081 acactacctt tcgccagatg gaacaagagc tggctgcatt tgccgctcat cttgagcaac
    13141 acaagctatt ggttgcccgc gtgttctctt tgccggaggt aaaaaaagag gatgagcata
    13201 atccgcttaa tcgtattgag gtaaaacaac atctcggcaa cgacgcgcag tcgctggcgt
    13261 tgcgtcattt ccgccattta tttattcaac aacagtccga aaatcgcagc agcaaggccg
    13321 ctgtccgtct gcctggcgtg ttgtgttacc aggtcgataa cctttcgcaa gcagcgttgg
    13381 tcagtcatat tcagcacatc aataaactca agaccacgtt cgagcatatc gtcacggttg
    13441 aatcagaact ccccaccgcg gcacgttttg aatgggtgca tcgtcatttg ccggggctga
    13501 tcacccttaa tgcttaccgc acgctcaccg ttctgcacga ccccgccact ttacgctttg
    13561 gttgggctaa taaacatatc attaagaatt tgcatcgtga tgaagtcctg gcacagctgg
    13621 aaaaaagcct gaaatcacca cgcagtgtcg caccgtggac gcgcgaggag tggcaaagaa
    13681 aactggagcg agagtatcag gatatcgctg ccctgccaca gaacgcgaag ttaaaaatca
    13741 aacgtccggt gaaggtgcag ccgattgccc gcgtctggta caaaggagat caaaaacaag
    13801 tccaacacgc ctgccctaca ccgctgattg cactgattaa tcgggataat ggcgcgggcg
    13861 tgccggacgt tggtgagttg ttaaattacg atgccgacaa tgtgcagcac cgttataaac
    13921 cacaggcgca gccgcttcgt ttgatcattc cacggctgca cctgtatgtt gcggattaac
    13981 gcccggcttt catactgccg accatctgtt ctggccgtac ccagctgtca aactcggctt
    14041 cgctaagata ccccagcgca agggccgcag cttttaaggt cagcccttct ttatgcgctt
    14101 ttttggcgat ctccgcggct ttgtcataac caatgtgggt gttaagcgca gtcaccagca
    14161 tcagcgattc attgagtaat tgattgattc gctcacgatt cggttcaata cccactgcgc
    14221 agtgtttgtt aaaactttcc atgccatctg ccagcaagcg caccgattgc aggaaattgt
    14281 ggatcaccat tggacggaag acgttcagtt caaagttacc ggaagcgcca cccatgttga
    14341 tcgccacgtc gttccccatt acctgacagc agagcatggt taatgcttca cactgggttg
    14401 ggttcacttt ccccggcatg attgagctgc ccggctcatt ttccgggatt gagatttcac
    14461 caattccgca gcgcgggcca gagaccagcc agcggacatc attggcgatt ttcatcagtg
    14521 acgcagccaa ccctttcaac gcgccgtgcg cctgaaccag ggcatcacag gtcgcaagcg
    14581 cttcaaattt gttcggcgcg gtaacaaacg gtgcacaggt aatgactgcc agttcatctg
    14641 ctacgcgacg cgcatactcc ggatgggtat ttagtccagt acccaccgct gtaccgccga
    14701 gagccagttc cgctacgtga ggcaggctgt attcgatatg tttgagatta tgctcgagca
    14761 tcgctaccca gccggaaatc tcctgcccca gtgttagcgg cgtggcgtcc tgcaagtggg
    14821 ttcgaccgat tttgacgata tcggcaaatg cacgcgattt ttcactcagt gtctgtgtca
    14881 gggttttaac ctgcggaatg agttgcttgc gcagcgccag cagcgccgca acgtgcatcg
    14941 ccgtcggaaa gacatcgttg gaactttggc ttttgttcac gtcgtcgtta gggtgaactt
    15001 tacgttccat cccgcgcacg ccgccgagta attcactggc ccggttagcc agcacttcgt
    15061 tcatgttcat gttactttgc gtgccggagc cggtctgcca gatagccagc gggaattcgt
    15121 cgtcatgctg tcctgccagt acttcatccg ccgcctgccg aatggcgctc gctttctctt
    15181 cagacaacaa gcctaaatct tcattaactt ttgccgctgc gcgcttggtt agcgccagcg
    15241 catgaatcag tgaggtgggc attttctccg tcgaaatgcg gaaatgctcc agcgagcgtt
    15301 gagtttgtgc gccccacagc ttatctgccg ggacatcaat cgcccccatc gaatcttttt
    15361 cgctgcgtac tgtattcatg gcctgctcct cacctgatta attttttctt tctgttttgc
    15421 cttcgttaag caacttttag ctcacttatt atttactatt tgataacaaa tgtttggtct
    15481 ttcgtgccat gtaaaaaaac cgccccgaag ggcggctctg tttatttcac acagcgagtg
    15541 cactgtgtga gttgtatctg ctggaagaag tcatttcctt tatcatccac aaggataaac
    15601 gccgggaaat cttccacttc aattttccag atggcttcca ttcccagttc cggatattca
    15661 acacattcca ggctcttaat acttccctgc gccaatacag cggccggacc accgatactg
    15721 ccaaggtaga agccgccgtg ttttttacag gcatccgtca cctgctggct gcggttgcct
    15781 ttcgccagca tgatcatact tccgccctgc gcttgcagtt gatcgacata agaatccatc
    15841 cgtccggcgg tcgttgggcc aagagaaccg gaggcataac cttccggcgt tttggccgga
    15901 cccgcgtagt aaatcggatg atctttaatg tactgcggca gcccttcacc gttatccatc
    15961 cgctctttca gtttggcgtg agcaatatca cgaccgacga taatcgtgcc gttaagcgat
    16021 aagcgtgtag aaacgggata ctgcgacaac tgtgcgagga tctctttcat cggacggtta
    16081 aggtcaacgc gcaccgcttc gccttctccc gctttacgca gctcttcagg gatatattta
    16141 cctggattat gttccagttt ttcgatccag atcccctgac ggttgatctt cgctttgata
    16201 ttacggtcag cagagcagga aacacccata ccaaccggac aggatgcgcc gtgacgtggc
    16261 aggcgaatca cgcgtatgtc gtgagcgaag tatttaccac caaactgcgc acccagaccg
    16321 agattttgcg cttcgatcag caattctttt tccagttcca catcgcggaa tgcctgaccg
    16381 tgctcattcc cttccgttgg cagttcgtca tagtatttag cggaagccag tttcactgtt
    16441 ttaaggttag tttctgcaga agttccacca attacgaacg caatatgata cggaggacag
    16501 gccgccgtac ccagcgtgcg catcttctca accaggtaat ttttcagttt ccccggcgtt
    16561 agtaacgctt tggtttcctg atagagatac gtcttgtttg ccgaaccacc acctttggcg
    16621 atacagagga atttgtactc gtcgccatca acggcataaa gatcgatctg cgctggcaga
    16681 ttggtgccgg tattcacctc tttatacata tccagcggcg cgttttgcga gtagcgcaaa
    16741 ttatcttcga tataagtgtt atagacaccg cgcgccagcg ccgcttcatc acagccaccg
    16801 gtccatacac gctgcccttt tttaccaaca ataatcgcag tgccggtatc ctgacaggtt
    16861 ggcagaacac ctttcgccgc gatgtcggag ttgcgcagga attgcagcgc cacatattta
    16921 tcattttcgc tggcttccgg gtcacgcaga atgtcggcca cctgttgttg gtgagccgga
    16981 cgcagcataa atgacgcatc atgaaacgcc tgacgcgcca acagagttaa cgcttcgggt
    17041 gcgactttca aaatctcctg cccttcaaat tcagatacgc taacgtgttc gctggttagc
    17101 aggtaatact cagtatcatc ttttttgagt ggaaaaggag cctgataatg aaagggtttg
    17161 tttgacattg ttctctcact tactgcctgg tttggttatg ctctgggcgg gtgttccgtt
    17221 gccctgttaa aagcgagtaa caatatccta cacacttttt taacaaaaac tgagactagt
    17281 acgacttttt gcggctccag gttacttccc gtaggattct tgctttaata gtgggattaa
    17341 tttccacatt aaaacaggga ttgatcatgc aaaaactcat taactcagtg caaaactatg
    17401 cctggggcag caaaacggcg ttgactgaac tttatggtat ggaaaatccg tccagccagc
    17461 cgatggccga gctgtggatg ggcgcacatc cgaaaagcag ttcacgagtg cagaatgccg
    17521 ccggagatat cgtttcactg cgtgatgtga ttgagagtga taaatcgact ctgctcggag
    17581 aggccgttgc caaacgcttt ggcgaactgc ctttcctgtt caaagtatta tgcgcagcac
    17641 agccgctctc cattcaggtt catccaaaca aacgcaattc tgaaatcggt tttgccaaag
    17701 aaaatgccgc aggtatcccg atggatgccg ccgagcgtaa ctataaagat cctaaccaca
    17761 agccggagct ggtttttgcg ctgacgcctt tccttgcgat gaacgcgttt cgtgagtttt
    17821 ccgagattgt ctccctactc cagccagtcg caggtgcaca tccggcaatt gctcactttt
    17881 tacaacagcc taatgccgaa cgtttaagcg aactgttcgc cagcctgttg aatatgcagg
    17941 gtgaagaaaa atcccgcgcg ctggcgattt taaaatcggc gctcgacagt cagcagggtg
    18001 aaccgtggca aacgattcgt ttaatttctg aattttaccc ggaagacagt ggcctgttct
    18061 ccccgctgtt actgaatgtg gtgaaattga atcctggcga agcgatgttc ctgttcgctg
    18121 aaacaccgca cgcttacctg caaggcgtgg cgctggaagt gatggcgaac tccgataacg
    18181 tgctgcgtgc gggtctgacg cctaagtaca ttgatattcc ggaactggtt gccaatgtga
    18241 aattcgaagc caaaccggct aaccagttgt tgacccagcc ggtgaaacaa ggtgcagaac
    18301 tggacttccc gattccagtg gatgattttg ccttctcgct gcatgacctt agtgataaag
    18361 aaaccaccat tagccagcag agtgccgcca ttttgttctg cgtcgaaggc gatgcaacgt
    18421 tgtggaaagg ttctcagcag ttacagctta aaccgggtga atcagcgttt attgccgcca
    18481 acgaatcacc ggtgactgtc aaaggccacg gccgtttagc gcgtgtttac aacaagctgt
    18541 aagagcttac tgaaaaaatt aacatctctt gctaagctta ttaaaggctt ataacacctt
    18601 caggcggcca gtccgcctga tttcatttta tggataatca ttatgaataa atcgctggta
    18661 gcggtaggcg tcattgttgc gctaggcgta gtctggacag gcggcgcatg gtatacaggc
    18721 aagaagattg aaacccatct cgaagacatg gtcgcgcagg cgaacgcgca actcaaactg
    18781 acagctcctg aatccaacct ggaagtgagt tatcaaaact atcatcgcgg cgtattcagc
    18841 agccagttgc aactgttggt gaaacccatt gccgggaaag aaaatccgtg gattaaaagc
    18901 ggtcagagcg tcatcttcaa cgaatcggtt gatcatggtc ccttcccgct tgcccagctt
    18961 aaaaaactga acctgatccc gtcgatggca tcaattcaaa ccacgctggt taataacgaa
    19021 gtaagcaaac cactgtttga tatggcaaaa ggtgaaacgc cttttgagat taactcgcgc
    19081 attggttaca gcggtgattc cagttccgat atttcgctca agccactgaa ttacgagcaa
    19141 aaggatgaaa aagtcgcctt tagcggcggc gagttccagt taaatgctga cagagacggc
    19201 aaagccatct ccctttccgg ggaggcgcaa agtggtcgga tagacgcagt taacgaatac
    19261 aaccagaaag tgcagttgac ctttaataat ctgaaaaccg acggttccag cacgctggca
    19321 agttttggtg agcgcgtagg aaaccaaaaa ctgtcactgg aaaaaatgac catttcagtg
    19381 gaaggcaaag aactggcact gctggaaggc atggagatca gcggtaaatc ggatctggtc
    19441 aatgacggta aaacgatcaa tagccaactg gattactcgc taaacagcct gaaggtacag
    19501 aatcaggatc tgggcagcgg caagctgact ttaaaagtcg gccagattga tggtgaagcc
    19561 tggcatcagt ttagccagca atataacgcg caaactcagg cgctgctggc acagccagaa
    19621 attgccaaca atcccgaact ttatcaggag aaagtgacgg aagccttctt tagcgccctg
    19681 ccgctgatgt tgaaaggcga tccggtgatt actatcgcgc cgctaagctg gaaaaacagt
    19741 cagggtgaaa gtgcgctgaa tctgtcgctg ttcctgaaag atccggcaac gactaaagaa
    19801 gcgccgcaaa cgctggcgca ggaagtagat cgttcggtta aatctctgga tgcgaaactg
    19861 accattccgg tggatatggc aactgagttt atgactcagg tagcgaagct ggaaggttat
    19921 caggaagatc aagcgaaaaa actggcgaaa cagcaagttg aaggtgcatc agcaatgggg
    19981 cagatgttcc gtctgaccac cttgcaggac aataccatca ccaccagcct gcaatatact
    20041 aacggtcaga taacgttaaa cgggcagaaa atgccactgg aagatttcgt tggtatgttt
    20101 gcgatgccag ctcttaacgt tccggctgta ccggcaattc cgcagcagta attcacggcc
    20161 aaaacgccgg agaattttca cttcggcgtt ttcatctgaa aacaaaccag agattgcctg
    20221 atgcgctacg cttatcaggc ctaccttttc ctgcaacact ttgaatttat gagtttttgt
    20281 aggctggata aggcgtttac gccgcatccg gcataaaaaa cgcgcacttt gtcaacaatc
    20341 tgaaacgccg gaggtttttc tctccggcgt tatttctgac ttcagcataa agtcatactg
    20401 aatactcatt ttcacatccc agtaattcag gcgatcgccc agcgtactgc catcgcgctt
    20461 ttcccgccct ttatcggcag caagaaagaa attcatccct tgtaacggcc cttcgctgac
    20521 ccgatagttc ataccaccta agatacccgc gccttcaatc cgcacatctc gtttactgga
    20581 ttcatagccg cgagtcacaa tctgtgcaaa tgtcagtgta aattgcggcg ttaaacgata
    20641 gttgacgccc aattgccagg actgcattcc ttcacggtta cgatcaagac ttaatgaaaa
    20701 gggatagcca atgtcggtat ccaccacggc accattcatt gcatggcgca aataaccacc
    20761 actgccaaat acgcttcccc acggcgcatt ccaggtcaat tgtccactaa ccaacgccgt
    20821 ttcattgggc tggctggtat tacgacttaa accttcgagc tgcgcgtaaa agccgagcaa
    20881 ctcccctttc aacgtataat tcgggtcgag cggtatgctt ttctcgagaa tactttgtat
    20941 ctgattggaa acatctttct gtaccgatgc ccccagagaa tagcgactgg tatcatcatg
    21001 ccaactggcg gctaaaaaag aacgtgcgcg atgtttatcg tattttcccg tctggctgtt
    21061 atacacccct tcatcacgaa taccggttgt tgaatcagtc cataaaaata ctcgtgaacg
    21121 ccacattccc cacagatcaa tatttggtgt taaagcccaa taacctaatg ctccttcaga
    21181 aactgtcggt aatgtacgtt gcgaactgcc agccattaac ggaatacgat aaggtaagct
    21241 accataacta taatccgtcc cgaagcgacc tgatttaagc aggctatttt cactgggttt
    21301 tatttttact gcccccaggg caaagccaaa acttttatca ccatcaagat accaccgggt
    21361 actcatatca gcacgagcac ctaatttata aacataatat gccccgcctt caacgccaat
    21421 aatattggaa taataaccac tattgaaatc gagcaatcct ccctgtaccc atgcgtaaat
    21481 atctcttccc gcgccagcac tgggcttatc cgccctgcgc aattcgtttt tcagtctgat
    21541 gcgtagtccc gcctcatcgt cagccagttg cgccgcataa gcagagataa ggccagaggc
    21601 agccgtcagg caaataacgg ccatggccac tatttttctc atagttgcac ctttgatgtt
    21661 ctgattattt ttattgatta ttaattagtg atatcgctga ttaattgctg ctgcactttt
    21721 ttacgattat caatttcaac cacgatttct ttgaattttt tatccgtaag cggataaaac
    21781 cagataataa cgaatgccag tagcataaat ccgcaaggta ctaaggcaat tgatgtgcgg
    21841 atgcccataa taacttccgg cgtttgcacc tgattggcaa tatatccgct taaccccaaa
    21901 ataaaggcag gaattgaacc tccgattgcc tgaccgcatt tacgggtaaa tgagaatagt
    21961 gaataggtga gcccttcaat tcgcacgccg gtcaggtatt caccgtattc tacggtatca
    22021 gcttccagtg cccacatcac tgtcatagta acgccctgac caattgaagc gatggccaac
    22081 gcaaccaacg ccaccggcag tgaccagacg gaaacccaga agaatagcag ataaccgcag
    22141 gttcccagca aagcgccaat caggaaggta ttctttttac cgatcctcgc gaccatcccc
    22201 ggcacaagcg gtgccgatgc cacagtacca accaggtttt gcaccagtac cagcacagtg
    22261 aacagcccgg tatcatttaa cacatagcgc acgtagaaca acgacgaggc gctgaccgca
    22321 aaggtcgaaa tcagcacaca cagcgcaccg atgcacaaca taaacagcgg gcgattccgt
    22381 ttcagggttt gcaaactgat cttcaatgac ggctgcgcaa cgatacgtac cacattctca
    22441 cgcgtcgatt tgaagcagat gaagtaaagc accattccgg caatcgccag cacaatcgtc
    22501 cagaaatggt ataccgacac catctcttcc gggctggagt tcttaatgct cggccctatc
    22561 agaaatgcca ggcagacaaa ggtcaatgaa gcggcaatcc cacgagccgc gcccagacgg
    22621 gcgcgggatt gtggttgttg ggtcatcgcg gtagcaagtg aaccataagg aatattcacc
    22681 aggctgtagc aaagcccgag gcccatgtag gtcaaatatg catacactac tttgcggcta
    22741 tggctccagt cggtcggtac ccagaatacc agcacgctga agatcattaa cggcgcagta
    22801 ccgaagagta aaaacgggcg gaattttccc cagcgggtat tcacactgtc caccactcgt
    22861 ccggcaaaga cgtcggcgaa ggcatcgaat acccgcacca gtaacagcat ggtgcccgcc
    22921 gcagcggcac cgacgccagc gacgtcggtg tagtaactca acaggaagag cacccccatt
    22981 gcgaaggcga agttattggc gacgtcaccg aggctgtagc cgacgatggt gcgccaggag
    23041 agttgttgat tcattgtttg cctccctgct gcggtttttc accgaagttc atgccagtcc
    23101 agcgtttttg cagcagaaaa gccgccgact tcggtttgcg gtcgcgggtg aagatcccct
    23161 tcttgttacc gccaacgcgc aatatgcctt gcgaggtcgc aaaatcggcg aaattccata
    23221 cctgttcacc gacgacggcg ctgacgcgat caaagacgcg gtgatacata tccagccatg
    23281 cacactgata ctcttcactc cacatgtcgg tgtacattga gtgcagcccg gctaacgtat
    23341 ccacgccgta ttcggtgatg ataatcggct gatgcagttt ctcctgccag gccagaagtt
    23401 ctttttccag taccttctct gccgtttcca aatcgccgct ttggacatac catccgtaat
    23461 aacggttcag gcacagcaca tcaaagagat cgctgatggt atcggtgtga gcgtcgcaga
    23521 acattacatt gacgcaggtg atcggacgcg tcgggtcgag tttacgcgtt gcttccgcca
    23581 gtggcgcgaa atattcccgt gcaccttgcg gacgggtatc cggttcgttg gcaatactcc
    23641 acatcaccac gcttgggtgg tttttgtcac gcgctatcag ctctttaatc gcctgtaagt
    23701 gcgcttgctg agtttccccg ttgactgcct cttcgctgta cagttctttc ggcttgttgc
    23761 ccgcttcgaa accaatgcct aaagagaggt taaagccgac agcagcagtt tcatcaatca
    23821 ccacgatgcc atgttcatct gcccagtcga gcatctcttc agcgtaaggg taatgcgagg
    23881 tacggtagga gttggcccca atccagtcca ttaatgcgtg gtcgtgcacc atcagcacgt
    23941 tatcgaatcc tttgccacgc aagtccgcat cttcatgacg accaaagcca gtaaagtaga
    24001 acggtttgtg gttaatcagg aactgttcgc ccttcactgc cactgaccgg atgccgacgc
    24061 gaagcgggta gatatcacac tctgtctggc ttttggctgt gacgcacagt tcatagagat
    24121 aaccttcacc cggttgccag aggtgcggat tcaccacttg caaagtcccg ctagtgcctt
    24181 gtccagttgc aaccacctgt tgatccgcat cacgcagttc aacgctgaca tcaccattgg
    24241 ccaccacctg ccagtcaaca gacgcgtggt tacagtcttg cgcgacatgc gtcaccacgg
    24301 tgatatcgtc cacccaggtg ttcggcgtgg tgtagagcat tacgctgcga tggatcccgg
    24361 catagttaaa gaaatcatgg aagtaagact gctttttctt gccgttttcg tcggtaatca
    24421 ccattcccgg cgggatagtc tgccagttca gttcgttgtt cacacaaacg gtgatacgta
    24481 cacttttccc ggcaataaca tacggcgtga catcggcttc aaatggcgta tagccgccct
    24541 gatgctccat cacttcctga ttattgaccc acactttgcc gtaatgagtg accgcatcga
    24601 aacgcagcac gatacgctgg cctgcccaac ctttcggtat aaagacttcg cgctgatacc
    24661 agacgttgcc cgcataatta cgaatatctg catcggcgaa ctgatcgtta aaactgcctg
    24721 gcacagcaat tgcccggctt tcttgtaacg cgctttccca ccaacgctga tcaattccac
    24781 agttttcgcg atccagactg aatgcccaca ggccgtcgag ttttttgatt tcacgggttg
    24841 gggtttctac aggacgtaac ataagggact cctcattaag ataaaaatac tggtcaacct
    24901 ttaatctgat tagttaacca attctgtgtt gcacacagtt atctgtgaga aacccatgga
    24961 agagaaatgg aaataaaaag aactgacacc gcgatcaaaa aaccaaaaac tgcaataccc
    25021 acagcgtgat attgatcgca ttaatcttta aaacggcgtt gacagaagcc ataaaataag
    25081 caaccgaacg ttcgttcgag catattggga acgataaact ctcatgacat atggctacgg
    25141 tgaatatttt tgggggggaa ttctccgatc ccggctagat tgtattttgt acagggaaat
    25201 taaagtatga aacgcgggca cagttatcac cagcccgcga aatgagagga tcaggatgcg
    25261 gttaagatac cgccaatcat cgcgcgtaat ccctgagcaa acgacgcttc acgcgccaga
    25321 tcttccgctt cgatatcggc cagtacaccg taggtcagat ccagcagtaa acgtgaagtc
    25381 attgacgtgt tgatgtctgg gcttatctcg cctttttgct gggcatcacg catccgctgg
    25441 gcaacaaatt ccgtgatcgt catatgctta tttttaagca tggcggcgac ctgtgggtta
    25501 cgcatccctt ccgccataat ttcgaccacc agcgcccgtt gcccaaaggc ttcatgggtg
    25561 agagagacaa tagactcgac catatagtca acaaaatgaa tccctttaat cggttcccgg
    25621 aaacgagcca gcgccctctc ctggtcctgt aagataatcg cctgaatcaa ggcttctttg
    25681 gagatgaaat ggtgatagag cgtcccggga ctaatggcgc aagatttaca gatggctttc
    25741 atcgaggcac tgtgaaatcc attttctgaa aaaatctctc tggcagcatt gaggatccgg
    25801 gtccgtgtcg gttgtgcttc agtctgcatg ttatccatca ttaaaccatt atagttagcg
    25861 tgaataacta gtttaccgcg ttgtcaggtg agcgcctatc attgctgttg aatagtgcga
    25921 aggcacactc tattcgtata cttttcactc tgaaattggt taaccaatag ccgtgatttg
    25981 gctgccagtt atttagcaga gacgttgatg agtttatccc ggtaaacaac acgcacggtt
    26041 gtttaccggt ccgttagtgt attaattgag ctcctgtacc ccaccaccgg agacggtgag
    26101 aatttgtccg cttacccagc tcgcagcagg cgagcaaagg aacagcgctg cgttagcaat
    26161 atcttgcggt tggcccagac gtctgatcgg cgtgtgctgt aacatttttt gttcaatttc
    26221 tggtgtaata acggatttca gggcatcggt taatattgcc cccggcgcaa tgccatttac
    26281 ccgaatattt ttttcaccca ggtcaaacgc catatttctg accagatgac tggctgcagc
    26341 tttagatgat gcataggaag tcatgtttat atttttattt tctgccgcca tagaagtgat
    26401 ggtcagaata acgccaccgc catttttttc catttctggc gcaacaagtt gtgacagatg
    26461 gaaaaaagaa aacacattca gttcataagc acggcgaaaa tccgccattg gcatatcaaa
    26521 cggtttaggt ccaccgccac cggcgttgtt aaccagaata tcaactttac ccagcttact
    26581 gatagcaaag tctgccagtg cagagagttc ctgttcggaa gtaatatcac aacggcaggc
    26641 aaatgcctga ccacccagtt gttgaatttc gtctacaaca tggttagctg cgtcggcgtt
    26701 aatatcactg accaccacag atgcgccagc tgtcgcgaat gtaatggcga tttctttacc
    26761 aatacctgca cccgcacctg tgatgatggc gcattttccg t
//
LOCUS       DAKISA010000052        26051 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.052, whole genome shotgun sequence.
ACCESSION   DAKISA010000052 DAKISA010000000
VERSION     DAKISA010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26051
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.052"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..675
                     /locus_tag="OVW45_002295"
     CDS             <1..675
                     /locus_tag="OVW45_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HCV5526726.1"
                     /translation="VVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSWLL
                     VLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSAGI
                     TFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTGEA
                     SGSLDLMLDNLAHHHRDNTMALADNLAALLEPTLLIITGGIIGTLVVAMYLPIFHLGD
                     AMSGMG"
     gene            complement(710..1753)
                     /gene="guaC"
                     /locus_tag="OVW45_002296"
     CDS             complement(710..1753)
                     /gene="guaC"
                     /locus_tag="OVW45_002296"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCV5526727.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(1909..1953)
                     /locus_tag="OVW45_002297"
     CDS             complement(1909..1953)
                     /locus_tag="OVW45_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5526728.1"
                     /translation="MIIKTLKMSARKRN"
     gene            1978..2598
                     /gene="coaE"
                     /locus_tag="OVW45_002298"
     CDS             1978..2598
                     /gene="coaE"
                     /locus_tag="OVW45_002298"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCV5526729.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            2598..3341
                     /gene="zapD"
                     /locus_tag="OVW45_002299"
     CDS             2598..3341
                     /gene="zapD"
                     /locus_tag="OVW45_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCV5526730.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            3351..3548
                     /gene="yacG"
                     /locus_tag="OVW45_002300"
     CDS             3351..3548
                     /gene="yacG"
                     /locus_tag="OVW45_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCV5526731.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(3648..4037)
                     /gene="mutT"
                     /locus_tag="OVW45_002301"
     CDS             complement(3648..4037)
                     /gene="mutT"
                     /locus_tag="OVW45_002301"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCV5526732.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(4097..6802)
                     /gene="secA"
                     /locus_tag="OVW45_002302"
     CDS             complement(4097..6802)
                     /gene="secA"
                     /locus_tag="OVW45_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCV5526733.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(6864..7376)
                     /gene="secM"
                     /locus_tag="OVW45_002303"
     CDS             complement(6864..7376)
                     /gene="secM"
                     /locus_tag="OVW45_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCV5526734.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(7607..8524)
                     /gene="lpxC"
                     /locus_tag="OVW45_002304"
     CDS             complement(7607..8524)
                     /gene="lpxC"
                     /locus_tag="OVW45_002304"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCV5526735.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(8625..9776)
                     /gene="ftsZ"
                     /locus_tag="OVW45_002305"
     CDS             complement(8625..9776)
                     /gene="ftsZ"
                     /locus_tag="OVW45_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCV5526736.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(9837..11099)
                     /gene="ftsA"
                     /locus_tag="OVW45_002306"
     CDS             complement(9837..11099)
                     /gene="ftsA"
                     /locus_tag="OVW45_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCV5526737.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(11096..11926)
                     /gene="ftsQ"
                     /locus_tag="OVW45_002307"
     CDS             complement(11096..11926)
                     /gene="ftsQ"
                     /locus_tag="OVW45_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCV5526738.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(11928..12848)
                     /gene="ddlB"
                     /locus_tag="OVW45_002308"
     CDS             complement(11928..12848)
                     /gene="ddlB"
                     /locus_tag="OVW45_002308"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCV5526739.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREDGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(12841..14316)
                     /gene="murC"
                     /locus_tag="OVW45_002309"
     CDS             complement(12841..14316)
                     /gene="murC"
                     /locus_tag="OVW45_002309"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCV5526740.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLIGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(14370..15437)
                     /gene="murG"
                     /locus_tag="OVW45_002310"
     CDS             complement(14370..15437)
                     /gene="murG"
                     /locus_tag="OVW45_002310"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCV5526741.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(15434..16678)
                     /gene="ftsW"
                     /locus_tag="OVW45_002311"
     CDS             complement(15434..16678)
                     /gene="ftsW"
                     /locus_tag="OVW45_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCV5526742.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(16678..17994)
                     /gene="murD"
                     /locus_tag="OVW45_002312"
     CDS             complement(16678..17994)
                     /gene="murD"
                     /locus_tag="OVW45_002312"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCV5526743.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIAQAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQLGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(17997..19079)
                     /gene="mraY"
                     /locus_tag="OVW45_002313"
     CDS             complement(17997..19079)
                     /gene="mraY"
                     /locus_tag="OVW45_002313"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCV5526744.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(19073..20431)
                     /gene="murF"
                     /locus_tag="OVW45_002314"
     CDS             complement(19073..20431)
                     /gene="murF"
                     /locus_tag="OVW45_002314"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCV5526745.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(20428..21921)
                     /gene="murE"
                     /locus_tag="OVW45_002315"
     CDS             complement(20428..21921)
                     /gene="murE"
                     /locus_tag="OVW45_002315"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCV5526746.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKAKDEATDGEIREMHGVPVIYLSQLNERLSALAGR
                     FYHEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENT
                     TGSAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHG
                     DMEHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWL
                     KAIDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKT
                     AARLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDR
                     DKGKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAV
                     TCAVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(21908..23674)
                     /gene="ftsI"
                     /locus_tag="OVW45_002316"
     CDS             complement(21908..23674)
                     /gene="ftsI"
                     /locus_tag="OVW45_002316"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCV5526747.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPGSIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(23690..24055)
                     /gene="ftsL"
                     /locus_tag="OVW45_002317"
     CDS             complement(23690..24055)
                     /gene="ftsL"
                     /locus_tag="OVW45_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCV5526748.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(24052..24993)
                     /gene="rsmH"
                     /locus_tag="OVW45_002318"
     CDS             complement(24052..24993)
                     /gene="rsmH"
                     /locus_tag="OVW45_002318"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCV5526749.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAVATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(24995..25453)
                     /gene="mraZ"
                     /locus_tag="OVW45_002319"
     CDS             complement(24995..25453)
                     /gene="mraZ"
                     /locus_tag="OVW45_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCV5526750.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAVGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT         6130 a         7405 c         6362 g         6154 t
ORIGIN      
        1 gttgtggcaa tgctgcattt tgttctgccg gagtttgccg ctatctataa gaccttcaac
       61 accccactac cggcactaac gcaggggatc atgacgctgg cagactttag tggcgaatgg
      121 agctggctgc tggtgttgtt cggctttctg ctggcgatag ccaataagtt gctgatgcgc
      181 cgaccgacct ggcttatagt gcggcagaaa ttgctgttac gcatcccgat tatgggttca
      241 ctgatgcggg gacaaaaact cacgcagatc tttacgattc tggcgctgac acaaagtgca
      301 ggcattactt ttttgcaggg cgtagagagc gtcagagaaa caatgcgctg cccgtactgg
      361 gtgcaacttc tgacacaaat ccagcacgat atcagtaacg gtcatcccat ctggctggcg
      421 ctaaaaaata ccggggagtt tagcccgctc tgtttgcaat tagtgagaac aggagaggca
      481 tccggctcgc tggacctcat gttagacaac ctcgcccatc atcatcggga taacacaatg
      541 gcgctggcgg ataacctcgc agccttactg gaaccgacgt tgctgatcat aacgggagga
      601 attatcggta cgctggtggt ggcgatgtat ctgccaattt tccatttagg cgatgcgatg
      661 agtgggatgg gataacgctg gcgtgttgct ccacaccagc gttggaagat tacaggttgt
      721 tgaagatgcg gttttcttgt tcctgcacac ggataaatgt ggtgcgcttg gtcagttctt
      781 tcaggcgtga agccccaacg tatgtacaag ctgaacgcag gccgcccaaa atatcacgcg
      841 cggtattttc aaccgggcct cgcagcggca gcttaacggt tttaccttct gctgcgcgat
      901 attccgcaac gccgccaacg tgacgtttca tcgcagactc ggagctcatg ccgtagaaca
      961 gcataaattt ctcgccgttc tcctcaacga tgcgaccgcc gctctcttcg tggcccgcca
     1021 gcatgccgcc aagcatgacg aaatcggcac cgccgccaaa ggcttttgcg acatcgcccg
     1081 gcgtggtgca gccaccgtcg ctgacgatca ttccgcccag accgtgcgcc gcatcggcac
     1141 attcgattac cgcagaaagc tgcggataac cgacgcctgt tttgacgcga gttgtacaaa
     1201 cagaacctgg gccaatgcca actttaacga tatcagcacc ggagaggata agctcctcac
     1261 acatttcacc agtcactacg ttgccagcac aaatggtttt ggtcggccac gcttcacgcg
     1321 ctttcgcaac gaactgcacg aagtgttcgg aataaccatt cgccacatca atacaaacga
     1381 agtttaatgc cgggttcagg tcgagaatct gtttagtttt ttcgaaatcc gcatcagacg
     1441 taccggtaga aaccatcaca tgtttcagca catcagcgga agaattgttg ataaacgctt
     1501 gccactcttc gacagaatag tgtttatgca cagcagtcaa aatatcaaaa gaagccagcg
     1561 cagaggccat agaaaatgta cctacggtgt ccatatttgc ggcgataatc ggcacgccgg
     1621 accagctctg acctgaatgt ttgaaggtga attgacgttc cagttcaaca tcggaacggc
     1681 ttttaagagt ggagcgttta gggcggatga gaacgtcttt aaaacctaac ttcagatctt
     1741 cttcaatacg catgtgcgga ttcctggggt taatggctat aagggtaaga gcgcaactcc
     1801 agtgacgcta tcatacgcac taatcaatgc ggcgcaagac tgcgaaattg tcttttttta
     1861 cgctacaatc ccataaattt acctggtaaa tagtgagcga acgtttcatc aatttcgctt
     1921 tcgtgcactc atttttaacg ttttgataat catgatgaat tcccgggaat ataaattatg
     1981 aggtatatag ttgccttaac gggaggcatt ggcagtggca agagtaccgt tgccaatgcg
     2041 tttgctgatc tcggaattaa cgtcattgat gccgatatta ttgcgcgtca ggtggttgaa
     2101 ccaggtgcac ctgcgctaca tgccattgct gatcactttg gcgctaacat gattgctgct
     2161 gatggaacat tgcagcgccg ggccttgcgc gagcggatct tcgccaaccc ggaagagaaa
     2221 aactggctta acgccctgct gcatccgctg attcagcaag agacgcaaca ccagatccag
     2281 caagctactt ccccctatgt actgtgggtt gtgccattgc tggtagaaaa ctcactgtat
     2341 aaaaaagcga atcgagtgct ggtggtggat gtcagcccag aaacgcaact taagcgcacc
     2401 atgcagcgcg atgatgtaac tcgcgagcat gtcgaacaaa tccttgctgc tcaggcaacg
     2461 cgcgaagccc gccttgccgt ggcagatgac gtcattgata ataacggcgc accggatgct
     2521 atcgcatcgg atgttgcccg cctgcacgca cactatttgc agcttgcgtc gcagtttgtc
     2581 tcacaggaaa aaccgtaatg cagacccagg tcctttttga acatccacta aatgaaaaaa
     2641 tgcgtacatg gctgcgcatt gagtttttga ttcagcaact caccgttaat ttacccatcg
     2701 ttgaccacgc tggcgcgctg catttcttcc gtaatgtcag tgaattactg gatgttttcg
     2761 agcgcggcga agtccgcact gagctgttga aagaacttga ccggcagcaa cgtaaactcc
     2821 agacctggat tggcgtgcct ggcgtggacc agagccgtat tgaagcatta attcagcagt
     2881 taaaagcggc ggggagcgta ttaatttccg cgccgcgtat cgggcaattt ctgcgtgaag
     2941 atcgtttgat tgctctggtg cgtcagcgac tgagcatccc aggcggctgt tgcagctttg
     3001 atttacctac attgcacatt tggctgcatc taccccaggc gcagcgcgac agccaggtag
     3061 aaacctggat tgccagcctg aacccgctca cccaggcact taccatggtg ctggatttaa
     3121 ttcgccagtc ggcccccttc cgtaaacaaa ccagtctgaa tggtttttat caggataacg
     3181 gtggcgatgc cgacttgctg cgcctgaatc tgtcgctcga ttcacagctt tatccgcaaa
     3241 tttccggaca taagagccgt tttgccattc gttttatgcc gctggacact gaaaacggac
     3301 aggtaccgga acgtctggat ttcgaactgg cctgttgcta aggagtaaaa atgtcagaaa
     3361 ctattacggt gaattgccca acctgcggga aaacggtggt gtggggtgaa atcagcccgt
     3421 ttcggccatt ttgctccaaa cgttgtcagc tgatcgacct cggagaatgg gctgctgaag
     3481 aaaaacgaat ccccagcagt ggcgatcttt ccgaaagcga tgactggagc gaagagccaa
     3541 agcagtgaca tttgtctgat gccgcacgta ggcctgataa gacgcggaca gcgtcgcatc
     3601 aggcatcttg tgcaaatgtc ggatgcggcg tgaacgcctt atccgaccta cagacgctta
     3661 agcttcgcaa ttaccggttc attggctggc ggaaaatcat cggcattaag accgactagc
     3721 gacatccact caccgggttg cccttcttta ccccacggct ccccttccca gctttcgacc
     3781 agccagaacc acagagttat atgcctgtcc gggaattcat attccagttt ttcaaatagc
     3841 gaaaaatgtt gtggggtaat cccgacttct tcctgaagtt cacgcaccac cgcctgttcc
     3901 ggcgtttcgc ccatttcaat tttaccgccg ggaaactcca gtttattcgc catgtgcgca
     3961 tctgctgcgc gacgcgttat aaagatttca ttgttctcgt tgcgaataat acctaccgca
     4021 atttgcagct ttttcattgt cttaaaccta taaaaaaggc gcagaatcct gcgcctttta
     4081 cttcaactgt tagcttttat tgcaggcgac catggcactg cttgtatttt ttaccagaac
     4141 cgcacgggca aggatcgtta cgtcctactt tgcgctcacc ggtttgcgcc gccagtgcag
     4201 ctgcggctgc agagtcgtca tcctgatggc taagctgctg catttgcgct aaacgctcgg
     4261 cttccatacg acgctgttgt tccagctcct caacctcttc aggcatacgt acctggactt
     4321 tgctcagcgt gctgataact tcatatttta acgactccag catcgctgca aacatggaga
     4381 acgattcacg tttgtattcc tgcttcggat ctttctgtgc atagccacgc aggtggatac
     4441 cctgacgcag atagtccatc gccgctaggt gctctttcca cagagagtcg agagtttgca
     4501 gcatgacgcc tttttcgaag tgacgcatca tctcagcacc aaccacttct tctttacgct
     4561 gatacacttc gatggactgc gccagaattc gctcacgcag cgtctcttca tgcagttctg
     4621 gttctttatc cagccactcg gcaattggca aatcgaggtc gaaatcgttc ttcagacgtt
     4681 cctgcagccc cggaatatcc cacatttctt ccagtgactg tggcggaatg taggcatcaa
     4741 tggtcgcttt gaacacatct tcacgaatgc tgttgatggt ttcgctcaca tcgctgacat
     4801 ccagcagttc gttacgctgg gagtaaatgg cgcgacgctg atcgttagcc acgtcatcat
     4861 attccagcag ttgcttacga atgtcgaagt tacggctttc aactttacgc tgggcgttgg
     4921 caatcgcttt agtcacccac gggtgttcaa tggcttcgcc tggcttcata cccagtttac
     4981 gcatcatgcc ggatactcgg tcggaagcaa aaatacgcat cagcgcatct tccatcgaca
     5041 ggtagaaacg ggaagaacca gcatccccct gacgaccaga acgaccgcgc aactggttat
     5101 cgatacgacg ggattcgtga cgctcggtac cgatgatatg caggccacct gcttccagta
     5161 ccgcatcgtg acgtacctgc cagtcggctt taattttttc aatttgctct gcggtcggat
     5221 tttccagcgc ggcaacttct gcctgccagc taccaccgag cacaatatct gtaccacgac
     5281 ccgccatatt ggtcgcgata gtcaccgcag ccggataacc tgcctgagca acaatcgccg
     5341 cttcgttggc gtggaatttg gcgttcagga cgttgtgctt aataccggct ttggtcagtt
     5401 cgtttgacac cagctccgat ttttcgatgg agatagtacc caccagcacc ggctggcctt
     5461 tcgcagtacg ttctttgata tcttcaatga tcgcctgaat tttttccgct tcagtcatgt
     5521 agaccaggtc cggcagatct ttacgaatca ttggacggtt ggtcggaaca acgacggtat
     5581 ccagcttgta gatggagcta aattcgaaag cttcggtatc agcagtaccg gtcatccccg
     5641 ccagtttttc atacagacgg aagtagttct ggaaggtgat cgaagccagc gtttggtttt
     5701 cgttctggat ctgcacacct tctttcgctt ccacagcctg gtgcagacca tcggaccagc
     5761 gacggccctg catggtacga ccggtgtgtt cgtcaacgat gataacttca ccatctttaa
     5821 cgatgtagtc gacgtcgcgg gtaaacagcg catgagcgcg cagcgccgcc gttacgtggt
     5881 gcatcagcat gatgttggcc ggagagtaca gagactcccc ttcatccatg atgccctctt
     5941 tcaccagcag ttcttcaatc agcaccagac cacgttcggt caggttcacc tggcgagatt
     6001 tttcgtccac cgagaagtgg ccttcgccct ggaaggtttc ggagtcttct ttttcctgac
     6061 ggatcaggtg cggaataatt ttattcacgc gtttatacat ttccgagctg tcttctgccg
     6121 ggccggaaat gatcagcggt gtacgcgctt catcgatcag gatggagtcc acttcgtcca
     6181 ccagcgcata gtgcagttta cgctgtacac gttcttcagg gctgaacgcc atattgtcgc
     6241 gcaggtagtc aaagccgtat tcgttgttcg taccgtaagt gatgtcagct gcgtaagctt
     6301 cgcgctttgc cggtgctggc atgcccggca ggttgatacc gacagtcagg ccaaggaatt
     6361 caaacagcgg acggttgttt tcggcgtcac gttgcgccag gtagtcgttg acggtaacta
     6421 cgtgcacgcc tttaccggtt agtgcgttca ggtaagcagg cagcgttgcg gtcagggttt
     6481 taccttcacc ggtacgcatt tcggcgatgc agcgttcgtt aagaaccata ccgccgagta
     6541 actgaacgtc gaagtgacgc ataccaaaga cgcgcttact tgcctcacgt accacggcga
     6601 aagcttccgg gatcagattt tccagcactt cgcctttttc cagacgtgca cgaaactctg
     6661 cggttttccc tttcagttct tcgtcggaga gtttttccat ctccggttcc atggcattga
     6721 tgatgttgac cactttgcgc atccggcgca gggtgcgatc gttacgacta ccgaaaactt
     6781 tagttaacaa tttgattagc ataataaaat ctcaaacgcc ccgcgttgcg gagttaataa
     6841 aatgaagtaa aggtttattg ttgttaggtg aggcgttgag ggccagcacg gatgccttgc
     6901 gcctggctta tccagacggg cgtgctgaat tttgcttgtg gggtaaaatg cgcataatca
     6961 atgcgataac ccttttcaga cggcgtgcct tcctgggtca gcagcgcgct gagcgtatcc
     7021 agtaatgcaa gatgttgcgc ctgaagaggc aaagattctt cagcaacggg cagtgtttgc
     7081 ggtgccattg cgaaagaaag atgacggatt accgtgcgaa tggcatgttg atgccagtaa
     7141 tcaacggaat agttcgaatt cgggcggcgt gtgttcgctt ccagcaaggc caattgacca
     7201 aagttaactt tggctgaagg ctcgtggttg cgggttgtcg cttttgcggg cgcgtttggt
     7261 tcggcggcgt tgctgagcgc aggcaaacct aaactcgccg caaccatccc taataagaga
     7321 tgcggccaga agtagcgttt accaaactgt cgccagcgcg tcagtattcc actcacgtta
     7381 ttgccatccc gatcgtattc aaacgtattt agcgcacaaa tcttatcatt aaagcctgca
     7441 gtccacagca cgaatgaaga taagttgtgc ggaaaagtgc ttaagaaaac agcgttcgca
     7501 ccgtttttta tagctcttat aaaattcaaa aaaaaatgac tggttcacct ggccggagag
     7561 agtgccagat ttgccagtcg aattttatac gacagtataa atgtcgttat gccagtacag
     7621 ctgaaggcgc tttgaaggcc aacggcagtt ctgcgtcgtc ctggaaggtc acatattccc
     7681 aggcttcctg tttcgccagg acagcctgca gcagtttgtt attcagtgca tgaccggatt
     7741 tataagcggt aaatgcacca ataatattgt gaccacacat gaacaagtca ccgatcgcat
     7801 cgagcatttt gtgacgcaca aattcgtctt caaaacgcag gccgtcttcg ttcagtacgc
     7861 gataatcgtc aacaacgatg gcacaatcga agctgccgcc caggcacaaa ccacgggact
     7921 gcagatattc gatatcacgc atgaaaccga acgtacgcgc acggctgatc tggcgcataa
     7981 acgcatcagc ggagaagttc atcgcatagc gctggttgct ggaatcaata gccggatggt
     8041 taaaatcgat ggtgaaatcc agcgaaaaac cattgtacgg cttaaattca gcccacttat
     8101 cgccatcttc gacacgaaca gtctctttga tgcgaacaaa ttttttggcg cagttcaact
     8161 cgtcgatacc ggcgtcaagc agcaggtata caaacggagc ggcgctgccg tccatgatcg
     8221 ggatttccgg cgcgttaact tcgataacaa tgttatcgat gcccaggccc gcgagagcag
     8281 cattgaggtg ctctacggtt gaaatccgta catcatgctc gttgaccaga cacgtacaga
     8341 gcatggtatc acgcacagat ttggcatcgg ccgggaaatc taccggtgga ttcaagtcgg
     8401 tgcgacgata gatgaccccg gtgttggccg gcgcagggcg taacgtcaga gtgactttct
     8461 tgccggtatg taaaccgaca cccgtcgcct gaacgatacg tttaagtgtc ctttgtttga
     8521 tcatcgtatt atctcgccaa attacctatc caaccgaagt gtactataca ttcggcgggc
     8581 cagtttagca caaagagcct cgaaacccaa attccagtca attcttaatc agcttgctta
     8641 cgcaggaatg ctgggatatc cagataatcc ggctctttcg cagtttgcgg cgcattgtca
     8701 ttcacgactt tagcaaccgg cttctgctcc tgggtcagcg gagccatccc atgctgctgg
     8761 tagcgatcca tcactggctg ctgaacctgc ttattggtca ccagagtgat ttcaggacgt
     8821 ttgtccatgc cgatacctgt cgcaacaacg gttacgcgca gctcgtcatt catatccggg
     8881 tcaagagaag taccgataac cacagtcgcg ttgtcggaag caaatgcacg gatggtgtta
     8941 cctaccgttt cgaactcatc cagacgcagg tcgaagcccg ccgtgatgtt aaccagcacg
     9001 ccgcgcgcgc cagacaggtc gatatcttcc agcagcggag aagagatagc catttcagca
     9061 gcttcttccg cacggtcttc accgctcgcc acgccagaac ccatcattgc gtagcccatc
     9121 tcagacatta cggtgcgtac gtctgcaaag tccacgttca tcaaacccgg acgagtaatc
     9181 agttcagcga taccttgcac agcgcctttc agtacatcgt tcgctgcgcc aaacgcatcc
     9241 agcagggaga taccgcggcc cagaactttc agcagtttgt cgttcgggat agtgatcaga
     9301 gagtccacat gcttggacag ttcagtgatc ccctgctccg cgaatgccat acgcttcttg
     9361 ccttcaaagt tgaaaggctt agtgacgaca gcaacggtca ggatacccaa atcttttgcc
     9421 acttcagcga cgactggtgc tgcacctgta ccggtaccac cacccatacc cgcagcaata
     9481 aagaccatgt ctgcaccttc cagcgccgca cgcaatgcat cgcgatcctc atcagccgca
     9541 ttgcggccaa cttctggatt agcgccagcg cccagtcctt tggtgatacc gctaccgatt
     9601 tgaatcgtct gtccaaccgc tgttttacgc agcgcttgtg catcggtatt taccgcgaag
     9661 aattcaacac cttcaatacg ctcgcgcacc atgtgttcaa cagcattacc gccgccgccg
     9721 ccgacgccga tgactttaat caccgcgtca ttggtaagtt ccattggttc aaacatagtt
     9781 tctctccgat ttgtgcctgt cgcctgaggc cgtaatcatc gtcggcctca taaaaattaa
     9841 aactcttttc gcagccaact attgagtcgc ttgatccacg agccaactga tgctgtaaca
     9901 cgtttttcta cttcagcttc accgttaaga tgtgactctt tcccatagtg aagcaatccc
     9961 accgccgtcg aataatacgg ctcctgagca taatccgtta aaccggtaat gttcagcggc
    10021 gcgccgatac gcacttgcgt atgaaacacg cgctgagcac aggctgcaag accttcgatc
    10081 tgcgctgcgc caccggttaa tacaatgcct gccgccaggt gatgtttaac cccttgttgg
    10141 cgaagctttt cctgcaactg caatatctct tcgttgacca ggttgagcag ctcggtatag
    10201 cgcggctcga tcacctctgc cagggtctga cgttgcagac tccgtggcgg acgaccgcct
    10261 acgctcggca cttccacgct ctcatctttt ccaacgatgg aacccagcgc acaaccgtgg
    10321 cgaactttaa tcgcttcggc gtcgcttggc ggcgtgccaa aggcgtaagc gatatcactg
    10381 gtcacgacat tgccagcata aggaattacc ttagtgtggc gcaatgcccc accggtataa
    10441 acggcgatat ccattgtacc accaccgata tcgacgacgc agacacccag ttcacgttca
    10501 tcttccgtca ataccgaata acttgatgcc agtccggcaa atatcagttg gtcaactttc
    10561 agcccacaac gttcaaccgc tttgacgatg tttttcgcca tatcgttgtg acatgtgatc
    10621 aggtgcactt ttgcctgcat ccgcacgccc gaaagtccta ccggattctt gatcccttcc
    10681 tgatagtcaa tcgcatactc ttgcgggatc acatgcagca cacgatgttc atcgcgcaca
    10741 cgcaccgatt tcgcggtatg gacgacgttt tccacatctt cttgcgtcac ttcttcttca
    10801 gaaataggca ccataccaat ttcattctgg cagctgatgt gcttaccaga aagcgccaga
    10861 tataccgaag agatctgaca atctgccatc aattctgcct ggtcaatggc gcgttgtacg
    10921 cacttgacca cggattcgag gtcgttcacc ccgcctttat ccataccacg tgacgggcag
    10981 ctgcccacgc caatgatatt gaccataccg tcgggcagaa cttcccctac taaagcggca
    11041 accttcgcgg taccaatctc cagtcctact accagttttc tgtccgtcgc cttgatcatt
    11101 gttgttctgc ctgtgcctga ttttgttgct gagtagattc ctctggcggc aagggcgccc
    11161 agcctactgc cgctccagag tcataacgca aatcaacgta gctaatccgt ttgccatcgg
    11221 tttgcgcctg ctgctgtaaa accggataaa gttctacaaa gcgagccaaa cgtttcatcg
    11281 tatcgccccg gccaagattg agcttaatat cgttattcag cgtcaactgc caggaacgcc
    11341 gcgcggtcat cgccgcttcc ttcagagtaa atctgtcctt tgccagcatc tgccccattt
    11401 cgcgatagcc ctgcaacact tcattggcgc tgccttccgg gccatacagc attggaagca
    11461 cctgcttgct ggtgcgatct ggcggcacgc tgaaggtgtt tccttccgcg tctaccatat
    11521 gttgatcatt ccaccgcgca atcggcacat attcaaccag atgaatcttc aattcatcag
    11581 gccactgctt tctgacgctc acctgcttaa tccacggcag gcgttgttct atttgcgtct
    11641 ggatgatgtt gacatcctgg gtcataaagg tacccggctc acccaatgcc aggatcgact
    11701 gccggatatc gtcattacgc gtgtaatggc gttcaccggt caacaccagc tttgagagcg
    11761 gcaggcgttg cgcatcttcc atccagccca acacgaccca gccgctcacc aacactgtcg
    11821 ttaaaacggt cagcaggaaa aggatccccg ccagacgcgt tccattattg cggcgagaag
    11881 aaacctcttc ttcgctattt cgcgtgttca gagcagcctg cgacatatta gtccgccagt
    11941 tccagaattc gtactaccaa ctgcgagaag ctcatacctg cctgacgtgc cgccatcggc
    12001 accaggctgt ggctggtcat acccggtgag gtattggctt ccagcagata aaactgtcca
    12061 tcgctgtcca gcataacgtc aatacgtccc catcctttgc aacctaacgt cgtccatgct
    12121 ttcagcacta atgcctgcaa attggcctct tgtgacgctt ccagacctgc ggggcagaaa
    12181 tactgtgtct catcagagag atacttcgcc tcataatcat agaaggttcc ggacggttga
    12241 atacgtattg acggtaaaat ttcttcaccg agtatcgcaa ccgtgaactc cggcccactt
    12301 agccattttt caatcaatac ttcttcatcg tgctgaaatg ccaatcttaa tgcatcttgt
    12361 agagcatttt ctgctactac ttttgacatt cccacactgg aaccttcgcg gctcggctta
    12421 acgataaccg gcaaacccag agcagaaatt tctgctaact gcttatcgct caggcctttt
    12481 tcaaactctg cgcgggttaa cgctacccac ggcgcgaccg gtaaaccggc accttgccac
    12541 agcagtttgc tgcgtagttt atccattgaa agcgcagatg ccatcactcc gctgccggta
    12601 taaggcaagc ccatcagctc gagcatgccc tgcagcgtac catcttcacc gccgcgaccg
    12661 tgtagcgcga taaacacttt ctgaaaaccc atcgacttca gttgcgtcac gtcgacttct
    12721 ttcgggtcga caggatacgc atcaataccg tcttcacgca gcccggctaa caccgctgcg
    12781 ccagaattca gagaaacttc ccgctcagcg gaagtcccgc ccaacaggac cgcaatttta
    12841 tcagtcatgt tgttcttcct ccggagtttg cggcttcagt ttgatttcag ctaaagaacg
    12901 ggcaattttt ccgatattac cagccccctg aacgagaatc aggtcgttac cgattaatac
    12961 cggtgccagc atctcggcta cctgcgcagg gtccggcacc agaatgggat caattttccc
    13021 acgtccacga attgtgcgac acagcgaacg gctgtccgct cccggaattg gcgcttcgcc
    13081 agccggatac acttccagca tcaacagggt atcaacctgc gtcagcacat tggcgaaatc
    13141 atcatacagg tcgcgcgtac gggtaaaacg gtgcggctga aacagcatta ctaggttttt
    13201 atccggccag cctgcgcgcg ccgctttaat ggtggcgtca acttccgtcg ggtggtggcc
    13261 gtagtcatcg accagcattg ccgtaccgct tttaccattc actggctcca gcgggaattc
    13321 accgaggaaa tcaaaacggc gaccagtccc ctggaagctc tccagcgcac gcaaaatagc
    13381 ctcgtcgtca attccctctt ccgtagcaac cgcaaccgca gctgcggcgt tcagcgcgtt
    13441 atgacggcct ggcgcattca gggtgacgcg catcggctct ttgtcctggc gcagcagcgt
    13501 aaagtgcccc tgcgggccaa tctgctgata atcttctaca cgcacgtcgg catcttcgct
    13561 gaagccgtaa gtcgtggtct gacgtcccac acgcggtaac aattcgcgga tcaccggatc
    13621 atcaacacac atcaccgcac gaccgtaaaa cggcaggttg tgcagaaaat taataaaagt
    13681 ctgttttaaa ttctcaaagt cgccctggta ggtatccatg tggtcggctt cgatattggt
    13741 gacaatcgcc accatcggtt gcagatgcag gaacgatgca tcactctcat ctgcttcggc
    13801 aatcaggtac cgaccatgcc ccaaacgcgc atgaaccccc gccgctttta ccagcccgcc
    13861 gttaacgaag gttgggtcga gccccgcttc tgcgtagatg ctggaaacca tcgcggtggt
    13921 tgtcgttttg ccgtgcgttc ctgcaatggc gatgccatga cgaaaacgca ttaactcagc
    13981 cagcatttcg gcacgacgga tcaccggaat acgcgcttca tgagctgcga caatttccgg
    14041 gttatcggca gaaatggcgc tggaaacaac gaccacgctg gcatcacgta cgttttccgg
    14101 gcgatggttg aaataaatcg tcgctcccag attcattaac tgctgcgtga ccggatttgg
    14161 cgctaaatcg gaaccactga tctgataacc ttcattggcc agaacttcgg caataccgcc
    14221 cataccggca ccaccgatgc cgacaaaatg tatgtgccga acgcgacgca tttcgggcac
    14281 gatggaacgc agttttgcca attgttgtgt attcattctt tacgccatta acttcttaaa
    14341 ttcatacgat gcaaaaggca tcgctacaat tacgcccggg cagcccggct cacttcattt
    14401 gccactcgct cggtggcatc cggaatggat gcagcgcggg cgcgttctgc catggttaat
    14461 aaggtttctc gcgaccaccc ggccagggtg ttggcgacag catccacgct aagctgtggc
    14521 tgctcgataa ttttggctgc gcccgctttt tccagcggta gcgcattcca gtattgctgg
    14581 cggtctttat gttgaaacgg cacaaacaac gccggtagtc ctgccgcggc gatttcactc
    14641 accgttaaag caccggagcg gcaaacgacg acatccgccc acgcatacgc cgccgccata
    14701 tcatcaataa attccgtcac tttatgctgc ggttgccccg cttcggcata cgcctgttca
    14761 acggattgtt gcgaaccttt accgctctga tgccagatag tgactgaatc acccagtttc
    14821 gcagcaacct gcggcattgt ctggttaaga atgcgtgcgc cctgggaacc acccactacc
    14881 agcacacgaa ccggaccttc acgtccagcc aaacgttgct gcggcaacgg cagcgccagc
    14941 acatcggtac gcaccgggtt acccaccact tccgcattag ggaaagcgcc tggaaacgcc
    15001 tgcatcactt tggtggcaat cttcgccagc catttattgg ttaagcccgc aataccgttt
    15061 tgttcatgaa gtacaaccgg aatgcctaac gaccacgcgg ccagaccacc tggacctgat
    15121 acatagccgc ccataccgag taccacgtca ggtttgtacg ctttcataat cgcccgcgcc
    15181 tgacgccagg cgttgaagat acgcagcggc gcagctatca gtgcttttat accttttcca
    15241 cgcagaccag agatacgaat gaaatcaatt tcgatgccat gttttggcac taagtccgct
    15301 tccatacggt cggcagtccc cagccagcga acttgccaac cctgagccat tagatggtgc
    15361 gcaaccgcca gtcccgggaa tacatgtcca ccggttccgc ctgccatcac cattaatcgc
    15421 tttccttgac cactcatcgt gaacctcgta caaacgcctg cgctttctcc agacgcgttt
    15481 cataatcaat acgcaacagc atcatgatgg ctgtcgacat aatcagtaag ctcgaaccac
    15541 cgtaactgat cagcggcaat gtcagacctt tggtcggtaa catccccgcc gccgcgccta
    15601 cgttaaccag cgcctggaag ctaaaccaga tgccaataga acaggcgaga aaaccggaaa
    15661 aacggtggtc aatttctaac gctttgcggc caatcgacat cgcgcgaaaa gcgacgaaga
    15721 ataccattaa aagtgccagc accacaccga cataccccag ttcttcgccg ataatggcga
    15781 aaataaagtc agtgtgcgct tccggcagat actccagttt ttgtaccgag ttacctaaac
    15841 cttgtcccca aagttcgccg cgaccaaacg ccatcagcga ttgcgttaac tgatagccgc
    15901 tgccaaaggg atcttcccac gggttccaga atgcggtaac acggcggata cggtacggtt
    15961 cggcgagtat cagcaacaca accgctgaaa tgcccatacc gataatggca atgaactgcc
    16021 acaatttcgc tcccgccagg aacaacatcg ccagcgtagt cacaaacaac accaccaccg
    16081 taccaaggtc tggctgtgcc agcagtaaca ctgccaacac cagaatcacg cccatcggtt
    16141 tcaggaagcc gcgcaggtta ttacgtactt cgtcgccttt acgcaccaga tagttggcga
    16201 tataacaaaa cagcgacagt tttgtcagct ccgcaggctg gatacgcagc aaaccgagat
    16261 cgatccaacg cgatgcccct ttaaccgagc tacccactac cagcacgatc atcagcagga
    16321 tgatagatcc gagcagcatc gtggcactgt agcgttgcca gaactccatc ggcagacgca
    16381 gcgtaatgat cgccagaatg aatgccagga tcagatagac gccatcacgc ttcgcgaaga
    16441 agaacggatc gttggttaag cgttgcccta tgggcattga cgccgaggtc accatgataa
    16501 agccaatcgc cgcaaggccg aaggtcagcc acagtaaggt gcgatcgtac atgatcaggc
    16561 tgtcggtatc tttttcccgc gagcccatca cccagccttt tagcgccgtg gagatccaga
    16621 ccaggatact gaatcctggc aggcgcggca ttttcaggcg agggagagat aaacgcatca
    16681 acctaactcc ttcgccagac gggcaaactc attgcctcgt tgttcaaagt tcttgaactg
    16741 atcaaggctg gcacaggccg gggagagcag aaccatatcg cccagctgaa cacgcggagc
    16801 cagcaagcgc atcgcttgtt ccatggtttc ggtttgttct gccacttccg ggcgtagcgc
    16861 cgccagctgc gcaccgtcac gaccaaaaca gtacaaacgc acgttgtcgc cattcagata
    16921 gcgcgccagt ggactgaagt ccgccgattt accatcgccg cccagcaaca aatgcagcgt
    16981 gccgtctacc tgcaagccat tcaacgccgc ttccgtactg ccgacgttag tggctttcga
    17041 atcgttaatc cagcgtacgc cattgtgctc cagcaccact tcaaagcgat gcggcagacc
    17101 ggtgaaggtg gttaacgctt tcaggctgct ggcgcgcggc aaaccagctg cgtctgccag
    17161 tgccagtgct gccagcgcat tggtgtagtt atgctgcccg gaaagtttca tctctttcac
    17221 attcagcact ttctcgcctt taacccgcag ccaggtttcg ccctgctgat gattcaggtg
    17281 atagtcaccc atgttgacgc caaagctgac gcagcgttca tcagcaccgc gaatcggcat
    17341 tgttaaggca tcatcagcat tgacgacaca aactttcgca ttttcgtaaa tacgcaattt
    17401 tgccgcacgg tattgctgca aaccaaacgg atagcggtcc atatgatctt cagtcacgtt
    17461 cagaatggtc gccgccaccg cctgtaagct ggaagtggtt tccagctgga agctcgacag
    17521 ttccagcacg tacagttcgc actcagcatc aagcagcatc aacgctggca ggccaatatt
    17581 gccacccaca ccaacgttaa cccccgccgc tttcgccatt tcacccacta gcgtggtgac
    17641 cgtgcttttg ccgttagaac cggtaatcgc cacaatcggt gcttgtgctt cgcgacagaa
    17701 cagctcgata tcgccaacga tttcgattcc ggcatcagcg gcagcgctta aggatggatg
    17761 cgcctgtgca ataccgggac tggcgacaat cagatctgcc gccatcagcc attcatcatt
    17821 cagactgccc gtgtggcgtt ctacggcttc gggtaattta tccaggccag gcggtgtcat
    17881 acgcgtatcc ataacgcgcg gcgtcacacc gcgagcgagg aaaaagtcca cgcaggaaag
    17941 cccggtgagg cccaggccga taatgacgac atttttaccc tgataatcag ccatgattaa
    18001 cgtaccttca gcgttgccag accaatcaga accagcatca gcgaaataat ccagaaacgc
    18061 acaatgacgc gcggttccgg ccagcctttc agttcatagt ggtgatgaat cggtgccatg
    18121 cggaaaatac gttgtccgcg cagtttaaag gagccgacct gcaggatgac agaaagcgtt
    18181 tctaccacga acacgccccc cataatcacc agcaggaatt cctgacgtag cagtacggcg
    18241 ataatgccta acgcaccacc taacgccagc gaacctacat cgcccataaa gacctgcgcc
    18301 ggataggtgt taaaccacag gaagcccagt cctgccccga ctatcgccgt gcagacaatg
    18361 accagttccc ctgcgtgtcg cagatacggt atatgcaagt agctggcaaa gttcatattg
    18421 ccggtcgccc acgccaccag cgcaaaacca ccggcgacaa atacggtcgg cataattgcc
    18481 aggccgtcga gaccatcggt caggtttacc gcgttgccag tacccacaat gacgaagtaa
    18541 gccagcagaa tgtagaacag ccccagctgc ggcatcacat ctttaaagaa tgggaccacc
    18601 agctgcgttg cgggcgtatc tttgccggca aggtacaggg cgaaggcgac acccagcgca
    18661 atgaccgaca tccagaaata cttccaacga gcgatcaacc ctttggtgtc tttacgcacc
    18721 actttgcgat aatcatcaac aaagccaata acaccgtaac ctaccagcac caccaacacg
    18781 caccagacgt acggattgga cgggtaagcc cacagcagta cggagatcac aatcgccgtc
    18841 aggatcataa tcccgcccat ggtcggcgta ccgcgcttgc tgaagtgtga ttcaggaccg
    18901 tcgttacgca ccacctgacc aaaggaaagt ttttgcaaat gagctatcat acgcgggccc
    18961 atccacaatg agatgaacag cgcggtcagc aggctgacga tggcgcgaaa cgtcagatag
    19021 gaaaagacgt taaagccgga ataatatttg accaaatgtt cggccagcca aactaacatg
    19081 tcccattctc ctgtaaagcg cgtactacct cttccatggc ggcactacgt gaacccttaa
    19141 ctaaaatcgt aattacctgt tgctcagcaa tcagtgattt aagacgcgta attaacgcag
    19201 ttttatcagc aaaatgttcg ccaacgccgc tggcggtgct gatagcatgg ctttgtttac
    19261 ccacgcttaa cacgcggtca ataccagcag cttttgccgc ctcgcccacc tgtacatggc
    19321 aggcttcgct ttcagcgccc agttccgcca tatcgcccac caccagcacg cggtagcccg
    19381 gcatttcagc cagtacctga acagcggcgg tcattgaacc gacattggcg ttgtaggagt
    19441 cgtcgagcag caactggttt tctgccagtt ggatggggaa cagacggcct ggaacagctt
    19501 tcagatttgc cagccccgct ttgatagcat caagcgttgc gcccacggac atggagagcg
    19561 cagcggctgc cagcgcattc gcaatattgt gacgccccgg caacggcagc agaacatcga
    19621 cgctaccggt tggggtttgt agggtaaatt ccgtaccgtg cgaggtcaca tggatattgg
    19681 tggcggtgaa atcgctgttg gcggcattgg gtgagaaacg ccacactttg cgtgagccaa
    19741 ttacgctctg ccagttcagc cagtcgttgt tgtcggcgtt cataatggcg ataccgtttt
    19801 ccggcaggcc gctaaagatt tcacctttcg ctttcgcgac acccgcaagc gagccaaaac
    19861 cttccagatg cgccgctgcc aggttgttga ccagcgcagc ttccgggcga gtcagactca
    19921 cagtccaggc tatttcgccc tgatggttcg cgccaagttc aataactgcg taatcgtatt
    19981 ccggcgttaa gcgcaacaac gtcatcggta caccgatgtc gttgttgaga ttgcctgccg
    20041 tataaagcgt gttgccgcac tggcttaaaa tcgccgccgt catctcttta acggaggttt
    20101 tgccggagga acctgtcaga gcaaccacgc gcgccggaac ttgctggcga acccatgcag
    20161 ccagttcacc aaacgccaga cgcgtatcct tgacgattaa ctgcggcagg tcgatatcca
    20221 gcggacggct gaccagtagt gcgcccgcgc cgccagcttt cgcctggtcg gcaaaatcat
    20281 gagcatcgaa acgttcgcct ttcagggcaa caaacaggca gcccggcgtc agttttcggg
    20341 tatcagtggt tacagcatca agggtgatat ctgcaccttg cagttcaccg ttgagaatgt
    20401 cggtaagttg gctaagggtt acgctaatca tgcaatcacc cccagcagac gcgccaccgt
    20461 gacgcgatcg gagtagtcca gacgctgatt gccaacaatc tggtaatctt catggccttt
    20521 gcccgcgacc agtaccacat cattctcttt agcctgcata acggcgcaag tcaccgcttc
    20581 agcacggcct tccatcactt tggcatgtcc ggcatctaac attcccgcca gaatatcgtt
    20641 gatgatggca cgcggttctt cggtacgcgg gttatcgtcc gtcaccaccg ccacgtcagc
    20701 aaactcttcg gcaattgcgc ccatcagtgg acgcttacct ttatcgcgat cgccaccaca
    20761 gccaaagacg caccacagct tgcccgcaca gtgcaggcgc gccgcctgta aggctttttc
    20821 cagtgcatcc ggcgtatgcg cgtaatccac caccaccgtc ggtttgcctg gcgcagtgaa
    20881 cacttccata cgtccgcaaa ccggttgcag acgcgcggcg gttttcagca gatcagccag
    20941 tggatagccg agtgccagca gtgtcgccag cgcgagcagc aggttgctga cgttaaaagc
    21001 gcccatcaga tggctttcaa tttcgccatc gccccaactt gagctaaagc gaatcgtcgc
    21061 accgctgtcg tgatagttca cgtcgatcgc ttttaaccag cgtccgtgac agttcggatt
    21121 aatatgatct tccattgata ccgcaaccgc gtccggcagt tttgccagcc agcggcggcc
    21181 cacttcatcg tcggcgttaa taatcgcctg accgcaatga tgttcagaat aaagcagcca
    21241 tttcgcagct tcgtagtgtt ccatatcacc atgataatca aggtgatcgc ggcttaagtt
    21301 ggtaaagacc gacgccgcaa atttcaatgc cgccacacgg tgctgtacca gcccgtggga
    21361 ggaaacttcc attgcgcaaa acgtcgcgcc ctgctccacc agccccgcca gctcatgctg
    21421 aacatcgact gcggaaccgg tagtattttc tgtcgggatc actttcccaa gcagaccgtt
    21481 accaacggtg cccattaccg cgctggtttc gccaagcagt tggctccact gcgccagcag
    21541 ctgggtagtc gtggttttgc cgttggtgcc cgttacgcct acgagacgta aattgtcaga
    21601 gggttcatgg taaaagcggc ccgccagtgc agataaacgc tcattgagct ggctgagata
    21661 gatgaccggt acgccgtgca tttcacggat ttcaccgtcg gtcgcctcat ctttcgcttt
    21721 cgcctctgca ataatggcag ccacaccttg cgctatcgcc tgcgggatat atcgacgccc
    21781 gtccgcctga tgacctacta cagctacaaa gagatcgccc gccgcagcca cacggctgtc
    21841 gagtgtcatc tctcgcagtg ctcgcgaagg tgcgtctggc acccacggag caagaaggtc
    21901 gcgcaaatta cgatctgcca cctgtcccct cgccttgatt aatcacaaat tcatttttat
    21961 cgcccgttgt cagcgcatcc ggctcgatgt tcatggtacg caatacgccg cccatgatgg
    22021 caccaaagac cggcgcggaa acggcgccgc cgtagtattt acccgcctgc ggatcgttga
    22081 taacaacaac cagcgcgaag cgcggctgac tcgcaggcgc aacgcctgcg gtataagcaa
    22141 tatatttatt gatgtagcga ccgtccggcc cgaccttttt cgcggtaccg gttttaatgg
    22201 cgatacgata gcctttaatc gccgccttca cgccgccgcc gcctggtagc gccacgcttt
    22261 ccatcatatg caccacagtg cggacaatgg atcccgggaa gacacgttca ccgggaaccg
    22321 gggggtcaac tttggtaatc gacagtgggc gataaatgcc gtagctgccg atagttgcgt
    22381 agactcgcgc taactgtaac ggtgttacca ttagcccgta gccgaaagag aaggtggccc
    22441 tctctatgtc agaccaccgt tgtttttgag gatataagcc actgcgttct ccgaccaacc
    22501 ccaaattggt cgcttttccc agtccaaaac gtgagtaagt atctactaac gctgaggacg
    22561 gcatcgctaa cgccagcttg gaaacaccga cgttactcga cttctgtaat accccggtca
    22621 gggttaattc gctgtagcgt gccacgtctt tgatttcgtg gccgttaatt cgataaggaa
    22681 tggtattgag taccgagttt tcccgcacca cgccacgttg caacgcggtc attaccacca
    22741 tcggtttaac cgttgagccc ggttcaaaca cgtcggtgat ggtacggtta cgcatcgcct
    22801 ctttcggcgt gccgctcaga ttgttagggt tgtatgacgg gctgttagcc atcgccagca
    22861 cttcaccggt gttgacatcc accagcacgg cgctaccaga ttcagccttg ttaaaggcca
    22921 ccgcgttgtt cagttcgcga taaaccagcg cctgcaggcg ttcatcaata ctcagcgcca
    22981 ggttgtgcgc tgcctggctg tcagtagaag aaatatcttc aattacgcga ccatagcggt
    23041 ctttacgcac aatgcgctca cccggctgcc cggtaagcca tttatcgaaa ctcttctcaa
    23101 cgccctcaat cccttgacta tcgacgttag taaagccgat gaggtgagca gtcacttcgc
    23161 cggacggata gtaacggcga gactcttcac gcagatgaat ccccggcagt ttcagttttt
    23221 tgatgtagtc cgccatgtca gggttcacct gacgcgccag ataaataaag cgccctttcg
    23281 ggttggcgtt aatgcgggct gaaagctgat ccagcggaat attgagcgcg ttagccagcg
    23341 ccttccagcg gtcaccgacg ctgataccgc cagcgtcatg cacttctttc gggtcagccc
    23401 aaatcgcttt taccggcacg ctcaccgcta acgggcgacc agaacggtca gtaatcatgc
    23461 cgcgggaggt ggaaacttgc tgaacgcgaa gagaacgcat gtcgccctct ttcaccagca
    23521 tatccgggga gataacttgt aaccacgcta cgcgtccgag cagaaaagcc agcgccagga
    23581 gaatacagcc gcataacaac gcaaaacgcc aactgataaa gttggcatgt tcttcctgac
    23641 gttttggttt ctgcgttttc gccgctgctt tcatgcgtcg cgtttatcct tatttttgca
    23701 ctacgatatt ttcttgtgac ggatcaacat gctgcatttg cagcttttcc gtggcgatcc
    23761 tttccacccg gctatggtcg ccgagcgcat tctcttcaag gatcaggttg cgccattcaa
    23821 tgtctaaagc atctcgctcc agcaccagtt gttcgcgctg agcggtcagt aaacgggtat
    23881 ggtgcgccgt ggttaccaca gtcaccgccg tcaaaataat gcaaatgaac aggcagagtg
    23941 gcagcttccc aaatcgcaaa agatcgtcac cgataacacc aggcaatgca tggcgctcgt
    24001 ggcttcccat cgatccttta actttgctta gagcttctgt cactctgctg atcatgcatt
    24061 cgtcctctct gcaatacgca gaactgaact acgggcacga gggttctcag ccacctcttc
    24121 ttcgcccggc attaacttgc ctagtgctcg cagctgacgg ccacccagtt ttttgagctg
    24181 ctcttcagtc atcggtaacc ctgccggaac ttgcggaccg cggctgtttt cacgcataaa
    24241 acgtttcaca atacggtctt ccagcgagtg gaagctgatg atcgaaagcc gcccacccgg
    24301 ggccagcacg ttgagcgagc tttttagcgc ctgctctatc tcctccagtt cactgttgac
    24361 ccaaatgcgc accgcctgga aggtacgggt cgcgggatgt ttaaacttat ctttcaccgg
    24421 cgttgcaaca gccacgactt ccgccagttc tttggtgcgg gtcatcggct gttcgcggtt
    24481 acgctcgaca atggcgcggg caatgcgttt ggcaaaacgc tcttcaccat aggttttcaa
    24541 tacccaagcg atatcggctt cttctgcggt ttgtagccat tcagcggctg actgcccacg
    24601 ggttgggtcc atacgcatgt ccagcggacc atcgcgcata aaggaaaagc cgcgctcagc
    24661 atcatcaagt tgcggtgaag agacgccaag atcgaggaga atgccgtcga tcttgccgat
    24721 aagatcgcgc tcggcaacgt attcgcccag cgcggagaaa ggtccgtgga tgatggagaa
    24781 gcgcggatca tcaatagtct tcgcaacggc gatagcctgc gggtcgcgat cgatcgccag
    24841 caaacgcccc tcttcgccaa gctgcgagag gatcagacgt gagtgaccac cgcgaccaaa
    24901 agtcccatca atgtagatgc catcaggacg gatattgagg ccattaacgg cttcatccag
    24961 cagcaccgta gtatgtttat agttttccat cattttatag agacaagtcc tgcagtcgct
    25021 ccgataagtc tccggtagcc aactgctctg cgtcgatatc ttccttgacc tgttgatgcc
    25081 aggttgtttc atcccacagc tcaaacttgt tgaactgtcc aaccagcatc acttcttttg
    25141 tcagcccggc atgttgccgc agtactggcg cgattaacaa tcgacctgcg ccatccatct
    25201 gacattcgct ggcatgacct aacagtaggc gctgcacacg gcgctcaacc gggttcatgc
    25261 tcgacagacg cgataatttt tgctcgataa tttcccattc aggcaggggg taaagcagca
    25321 ggcacgggtg atgaatgtca atggtgcaaa ccatttgacc gacagcgttc tcaagcagct
    25381 gttcccggta acgggtaggc actgataagc gccctttgct gtcgagattg actaacgttg
    25441 ctccccggaa catgccagcc tcacccctta ttcccacttt gccccacaat ttcccactga
    25501 aaggagttta cggagcggag gaaaagcttg tcaagccagt cacaaggcat accgaggtaa
    25561 aaaacccgat gtttacggct aatatcagcg ttgtttattt tacgtccaac gtgcgcagca
    25621 aattaacgtt atgaatagct gtaagaaaaa atccctcatc gtcatcaccg ttttaaaatg
    25681 actcactatt ttccacagca aatataaagt gtcagtttgc gacgcgagcg gcattttagg
    25741 acatatcttc cccggttaac agtccctgtt gcgtctgtgt ggcgcttgat gccagcgggt
    25801 ttgcgcaaaa gcaaagaaaa gtgcttgtta aatcggcaat tcatctctgc atgtaacaaa
    25861 ataatgcaaa aacgcgctgt cgatatcact taattattgt gaatttcagc tggtgagtta
    25921 attaacgggc gggtttaaaa gaacttatca ataatagtca ggaatagtct tatttacttt
    25981 aaggatattg atgtccattc ccgtactcta cgcgccaggt aatgactcca acttattgat
    26041 agtgttttat g
//
LOCUS       DAKISA010000053        26049 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.053, whole genome shotgun sequence.
ACCESSION   DAKISA010000053 DAKISA010000000
VERSION     DAKISA010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26049
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.053"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            15..485
                     /locus_tag="OVW45_002320"
     CDS             15..485
                     /locus_tag="OVW45_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HCV5526751.1"
                     /translation="MVDQNLPWSASLKVGDKKLPVVVVRVGEASVDVLVGQQTWTLTH
                     KWFESVWTGDYLLLWKMSPEGESTITRDSSEEEILWLETMLNRALHISTEPSAEWRPL
                     LVEKIKQFQKSHHLKTDGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK"
     gene            487..906
                     /gene="gspB"
                     /locus_tag="OVW45_002321"
     CDS             487..906
                     /gene="gspB"
                     /locus_tag="OVW45_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HCV5526752.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWALWIVKAEVTVEAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPDVA
                     VENAPSSSEDEENTVKESDEKAGLRERVKNALNELER"
     gene            1144..1455
                     /gene="rpsJ"
                     /locus_tag="OVW45_002322"
     CDS             1144..1455
                     /gene="rpsJ"
                     /locus_tag="OVW45_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCV5526753.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            1488..2117
                     /gene="rplC"
                     /locus_tag="OVW45_002323"
     CDS             1488..2117
                     /gene="rplC"
                     /locus_tag="OVW45_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCV5526754.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     EVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2128..2733
                     /gene="rplD"
                     /locus_tag="OVW45_002324"
     CDS             2128..2733
                     /gene="rplD"
                     /locus_tag="OVW45_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCV5526755.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2730..3032
                     /gene="rplW"
                     /locus_tag="OVW45_002325"
     CDS             2730..3032
                     /gene="rplW"
                     /locus_tag="OVW45_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCV5526756.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3050..3871
                     /gene="rplB"
                     /locus_tag="OVW45_002326"
     CDS             3050..3871
                     /gene="rplB"
                     /locus_tag="OVW45_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCV5526757.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            3888..4166
                     /gene="rpsS"
                     /locus_tag="OVW45_002327"
     CDS             3888..4166
                     /gene="rpsS"
                     /locus_tag="OVW45_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCV5526758.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4181..4513
                     /gene="rplV"
                     /locus_tag="OVW45_002328"
     CDS             4181..4513
                     /gene="rplV"
                     /locus_tag="OVW45_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCV5526759.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            4531..5232
                     /gene="rpsC"
                     /locus_tag="OVW45_002329"
     CDS             4531..5232
                     /gene="rpsC"
                     /locus_tag="OVW45_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCV5526760.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5245..5655
                     /gene="rplP"
                     /locus_tag="OVW45_002330"
     CDS             5245..5655
                     /gene="rplP"
                     /locus_tag="OVW45_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCV5526761.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5655..5846
                     /gene="rpmC"
                     /locus_tag="OVW45_002331"
     CDS             5655..5846
                     /gene="rpmC"
                     /locus_tag="OVW45_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCV5526762.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5846..6100
                     /gene="rpsQ"
                     /locus_tag="OVW45_002332"
     CDS             5846..6100
                     /gene="rpsQ"
                     /locus_tag="OVW45_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCV5526763.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6265..6636
                     /gene="rplN"
                     /locus_tag="OVW45_002333"
     CDS             6265..6636
                     /gene="rplN"
                     /locus_tag="OVW45_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCV5526764.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6647..6961
                     /gene="rplX"
                     /locus_tag="OVW45_002334"
     CDS             6647..6961
                     /gene="rplX"
                     /locus_tag="OVW45_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCV5526765.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            6976..7515
                     /gene="rplE"
                     /locus_tag="OVW45_002335"
     CDS             6976..7515
                     /gene="rplE"
                     /locus_tag="OVW45_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCV5526766.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            7530..7835
                     /gene="rpsN"
                     /locus_tag="OVW45_002336"
     CDS             7530..7835
                     /gene="rpsN"
                     /locus_tag="OVW45_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCV5526767.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7869..8261
                     /gene="rpsH"
                     /locus_tag="OVW45_002337"
     CDS             7869..8261
                     /gene="rpsH"
                     /locus_tag="OVW45_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCV5526768.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8274..8807
                     /gene="rplF"
                     /locus_tag="OVW45_002338"
     CDS             8274..8807
                     /gene="rplF"
                     /locus_tag="OVW45_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCV5526769.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGVDKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            8817..9170
                     /gene="rplR"
                     /locus_tag="OVW45_002339"
     CDS             8817..9170
                     /gene="rplR"
                     /locus_tag="OVW45_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCV5526770.1"
                     /translation="MDKKFARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9185..9688
                     /gene="rpsE"
                     /locus_tag="OVW45_002340"
     CDS             9185..9688
                     /gene="rpsE"
                     /locus_tag="OVW45_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCV5526771.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9692..9871
                     /gene="rpmD"
                     /locus_tag="OVW45_002341"
     CDS             9692..9871
                     /gene="rpmD"
                     /locus_tag="OVW45_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCV5526772.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9875..10309
                     /gene="rplO"
                     /locus_tag="OVW45_002342"
     CDS             9875..10309
                     /gene="rplO"
                     /locus_tag="OVW45_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCV5526773.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10317..11648
                     /gene="secY"
                     /locus_tag="OVW45_002343"
     CDS             10317..11648
                     /gene="secY"
                     /locus_tag="OVW45_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCV5526774.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFVGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11680..11796
                     /gene="rpmJ"
                     /locus_tag="OVW45_002344"
     CDS             11680..11796
                     /gene="rpmJ"
                     /locus_tag="OVW45_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCV5526775.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            11943..12299
                     /gene="rpsM"
                     /locus_tag="OVW45_002345"
     CDS             11943..12299
                     /gene="rpsM"
                     /locus_tag="OVW45_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCV5526776.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12316..12705
                     /gene="rpsK"
                     /locus_tag="OVW45_002346"
     CDS             12316..12705
                     /gene="rpsK"
                     /locus_tag="OVW45_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCV5526777.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12739..13359
                     /gene="rpsD"
                     /locus_tag="OVW45_002347"
     CDS             12739..13359
                     /gene="rpsD"
                     /locus_tag="OVW45_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCV5526778.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            13385..14374
                     /gene="rpoA"
                     /locus_tag="OVW45_002348"
     CDS             13385..14374
                     /gene="rpoA"
                     /locus_tag="OVW45_002348"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCV5526779.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            14415..14798
                     /gene="rplQ"
                     /locus_tag="OVW45_002349"
     CDS             14415..14798
                     /gene="rplQ"
                     /locus_tag="OVW45_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCV5526780.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            14905..15273
                     /locus_tag="OVW45_002350"
     CDS             14905..15273
                     /locus_tag="OVW45_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HCV5526781.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            15284..15709
                     /gene="zntR"
                     /locus_tag="OVW45_002351"
     CDS             15284..15709
                     /gene="zntR"
                     /locus_tag="OVW45_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HCV5526782.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15765..15983
                     /gene="arfA"
                     /locus_tag="OVW45_002352"
     CDS             15765..15983
                     /gene="arfA"
                     /locus_tag="OVW45_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HCV5526783.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(15980..16390)
                     /gene="mscL"
                     /locus_tag="OVW45_002353"
     CDS             complement(15980..16390)
                     /gene="mscL"
                     /locus_tag="OVW45_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCV5526784.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(16520..17896)
                     /gene="trkA"
                     /locus_tag="OVW45_002354"
     CDS             complement(16520..17896)
                     /gene="trkA"
                     /locus_tag="OVW45_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCV5526785.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(17918..19207)
                     /gene="rsmB"
                     /locus_tag="OVW45_002355"
     CDS             complement(17918..19207)
                     /gene="rsmB"
                     /locus_tag="OVW45_002355"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCV5526786.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVHDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19253..20200)
                     /gene="fmt"
                     /locus_tag="OVW45_002356"
     CDS             complement(19253..20200)
                     /gene="fmt"
                     /locus_tag="OVW45_002356"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCV5526787.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQESQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(20215..20724)
                     /gene="def"
                     /locus_tag="OVW45_002357"
     CDS             complement(20215..20724)
                     /gene="def"
                     /locus_tag="OVW45_002357"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCV5526788.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20854..21978
                     /gene="dprA"
                     /locus_tag="OVW45_002358"
     CDS             20854..21978
                     /gene="dprA"
                     /locus_tag="OVW45_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCV5526789.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQRREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            21950..22423
                     /locus_tag="OVW45_002359"
     CDS             21950..22423
                     /locus_tag="OVW45_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCV5526790.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            22452..22994
                     /locus_tag="OVW45_002360"
     CDS             22452..22994
                     /locus_tag="OVW45_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HCV5526791.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RLLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            22999..23571
                     /gene="tsaC"
                     /locus_tag="OVW45_002361"
     CDS             22999..23571
                     /gene="tsaC"
                     /locus_tag="OVW45_002361"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCV5526792.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            23576..24394
                     /gene="aroE"
                     /locus_tag="OVW45_002362"
     CDS             23576..24394
                     /gene="aroE"
                     /locus_tag="OVW45_002362"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCV5526793.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            24391..24648
                     /locus_tag="OVW45_002363"
     CDS             24391..24648
                     /locus_tag="OVW45_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCV5526794.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGGTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(24624..25178)
                     /locus_tag="OVW45_002364"
     CDS             complement(24624..25178)
                     /locus_tag="OVW45_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCV5526795.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
     gene            25651..>26049
                     /locus_tag="OVW45_002365"
     rRNA            25651..>26049
                     /locus_tag="OVW45_002365"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         6646 a         6151 c         6838 g         6414 t
ORIGIN      
        1 cttctttaca aacattggta gatcagaatt tgccctggag cgcatcgctt aaagtcgggg
       61 acaaaaaatt gcctgttgtg gtcgttcgcg tcggtgaggc tagcgttgat gtcctcgtag
      121 gtcagcaaac gtggacgctg acacataaat ggtttgagtc ggtatggacg ggtgattatc
      181 ttctgttgtg gaaaatgtcg ccggaagggg agagtaccat tacgcgtgat agcagcgagg
      241 aagagattct ctggctggaa acgatgttaa accgtgcatt gcatatttcg acagaacctt
      301 cggcggagtg gcgtcccttg ttggtagaaa aaataaagca atttcaaaaa agccaccact
      361 tgaaaactga cggtgttgtt ggcttttcaa cgttagtaca tctctggcag gtggcagggg
      421 agagtgccta cttatatcgg gatgaagcaa atatttcccc ggaaacaaca gtgaagggga
      481 aatgacatgt ttgaattcta tatcgccgcg cgtgagcaga aagaaacagg tcaccctggt
      541 atcttctccc gacaaaaaca cagcaccata atatatgtca tttgtttatt gctgatttgc
      601 ctgtggtttg caggaatggt gttggttggt gggtatgcca ggcagctatg ggcgctttgg
      661 atagtaaaag cagaagtcac tgtagaggct gaaacgcccg cttttaaaca atcaacacaa
      721 cactatttct tcaaaaaaca gccactcccc gtcgtcgaat ccgtcgagga ggaagatgac
      781 ccagacgtcg cggttgagaa tgctccctct tcttctgaag acgaagaaaa tactgtaaaa
      841 gaaagcgacg agaaagccgg tttaagagag cgtgtcaaaa atgcgctgaa cgaacttgag
      901 agataacccg aaggctgttt actttactgg caatacgctt gcgttcggtg gttaagtatg
      961 tataatgcgc gggcttgtcg tagttgacag caggttcaat ctgaacctca gttagccgaa
     1021 tttggctacc taacaatgct cccaatcggg gagctacgta agaacggtta cactctccca
     1081 tcaatcgtaa tgggtctgag gagtaatcat tttcgtttat aaaataattg gagctctggt
     1141 ctcatgcaga accaaagaat ccgtatccgc ctgaaagcgt ttgatcatcg tctgatcgat
     1201 caagcaaccg cggaaatcgt cgagactgcc aagcgcactg gtgcgcaggt ccgtggtccg
     1261 atcccgctgc cgacacgcaa agagcgcttc actgttctga tctccccgca cgtcaacaaa
     1321 gacgcgcgcg atcagtacga aatccgtact cacttgcgtc tggttgacat cgttgagcca
     1381 accgagaaaa ccgttgatgc tctgatgcgt ctggatctgg ctgccggtgt agacgtgcag
     1441 atcagcctgg gttaatcagg tcattgagcg attgagaggt tgaaacaatg attggtttag
     1501 tcggtaaaaa agtgggtatg acccgtatct tcacagaaga cggcgtttct atcccagtaa
     1561 ccgtaatcga agttgaagca aaccgcgtta ctcaggttaa agacctggct aacgatggct
     1621 accgtgctat tcaggtgacc accggtgcta aaaaagctaa ccgtgtgacc aagcctgaag
     1681 ctggccactt cgctaaagct ggcgtagaag ctggccgtgg tctgtgggaa ttccgcctgg
     1741 ctgaaggcga agagttcact gtaggtcaga gcattagcgt tgaactgttt gctgaggtta
     1801 aaaaagttga cgtaactggc acctctaaag gtaaaggttt cgcaggtacc gttaagcgct
     1861 ggaacttccg tacccaggac gctactcacg gtaactcctt gtctcaccgc gttccgggtt
     1921 ctatcggtca gaaccagact ccgggcaaag tgttcaaagg caagaaaatg gcaggtcaga
     1981 tgggtaacga acgtgtaacc gttcagagcc ttgacgtagt acgcgttgac gctgagcgca
     2041 acctgctgct ggttaaaggt gctgtcccgg gtgcaaccgg tagcgacctg atcgttaaac
     2101 cagctgtgaa ggcgtaagga gatagcaatg gaattagtat tgaaagacgc gcagagcgcg
     2161 ctgactgttt ccgaaactac cttcggtcgt gatttcaacg aagcgctggt tcaccaggtt
     2221 gttgttgctt atgcagctgg tgctcgtcag ggtactcgtg ctcagaagac tcgtgctgaa
     2281 gtaactggtt ccggtaaaaa accgtggcgc cagaaaggca ccggccgtgc gcgttctggt
     2341 tctatcaaga gcccgatctg gcgttctggt ggcgtgacct ttgctgctcg tccgcaggac
     2401 cacagtcaaa aagttaacaa gaagatgtac cgcggcgcgc tgaaaagcat cctgtccgaa
     2461 ctggtacgtc aggatcgtct gatcgttgtc gagaagttct ctgtagaagc gccgaaaact
     2521 aagctgctgg cacagaaact gaaagacatg gctctggaag atgtgctgat catcaccggt
     2581 gagctggacg aaaacctgtt cctggctgcg cgcaacctgc acaaggttga cgtacgcgat
     2641 gcaactggta tcgacccggt tagcctgatc gccttcgaca aagtcgtaat gactgctgat
     2701 gctgttaagc aagttgagga gatgctggca tgattcgtga agaacgtctg ctgaaggtgc
     2761 tgcgtgcacc gcacgtttct gaaaaagcgt ctactgcgat ggaaaaatcc aacaccatcg
     2821 tactcaaagt tgctaaagac gcgaccaaag cagaaatcaa agctgctgtg cagaaactgt
     2881 ttgaagtcga agtcgaagtc gttaacaccc tggtagttaa agggaaagtt aaacgtcacg
     2941 gacagcgtat cggtcgtcgt agcgactgga aaaaagctta cgtcaccctg aaagaaggcc
     3001 agaatctgga cttcgttggc ggcgctgagt aagtcggagg agtaatacaa tggcagttgt
     3061 taaatgtaaa ccgacatctc cgggtcgtcg ccacgtagtt aaagtggtta accctgagct
     3121 gcacaagggc aaaccttttg ctccgttgct ggaaaaaaac agcaaatccg gtggtcgtaa
     3181 caacaatggc cgtatcacca ctcgtcatat cggtggtggc cacaagcagg cttaccgtat
     3241 tgttgacttc aaacgcaaca aagacggtat cccggcagtt gttgaacgtc ttgagtacga
     3301 tccgaaccgt tccgcgaaca tcgcgctggt tctgtacaaa gacggtgaac gccgttacat
     3361 cctggcccct aaaggcctga aagctggcga ccagattcag tctggcgttg atgctgcaat
     3421 caaaccaggt aacaccctgc cgatgcgcaa catcccggtt ggttctactg ttcataacgt
     3481 agaaatgaaa ccaggtaaag gcggtcagct ggcacgttcc gctggtactt acgttcagat
     3541 cgttgctcgt gatggtgctt atgtcaccct gcgtctgcgt tctggtgaaa tgcgtaaagt
     3601 agaagcagac tgccgtgcaa ctctgggcga agttggcaat gctgagcata tgctgcgcgt
     3661 tctgggtaaa gcaggtgctg cacgctggcg tggtgttcgt ccgaccgttc gcggtaccgc
     3721 gatgaacccg gtagaccacc cacatggtgg tggtgaaggt cgtaactttg gtaagcaccc
     3781 ggtaactccg tggggcgttc agaccaaagg taagaagacc cgcagcaaca agcgtactga
     3841 taaattcatc gtacgtcgcc gtagcaaata attttagagg ataagccatg ccacgttctc
     3901 tcaagaaagg tccttttatt gacctgcact tgctgaagaa ggtagagaaa gcggtggaaa
     3961 gcggagacaa gaagcccctg cgcacttggt cccgtcgttc aacgatcttt cctaacatga
     4021 tcggtttgac catcgctgtc cataatggtc gtcagcacgt tccggtattt gtgaccgacg
     4081 aaatggtcgg tcacaaactg ggtgaattcg caccgactcg tacttatcgc ggccacgctg
     4141 ctgataaaaa agcgaagaag aaataaggta ggaggaagag atggaaacta tcgctaaaca
     4201 tcgccatgct cgttcttctg ctcagaaggt tcgccttgtt gctgacctga ttcgcggtaa
     4261 gaaagtgtcg caggctctgg atattttgac ctacaccaac aagaaagcgg ctgtactggt
     4321 caagaaagtt ctggaatctg ccattgctaa cgctgaacac aacgatggcg ctgacattga
     4381 cgatctgaaa gttacgaaaa ttttcgtaga cgaaggcccg agcatgaagc gcattatgcc
     4441 gcgtgcaaaa ggtcgtgcag atcgcatcct gaagcgcacc agccacatca ctgtggttgt
     4501 gtccgatcgc tgagactctg gagactagca atgggtcaga aagtacatcc taatggtatt
     4561 cgcctgggta ttgtaaaacc atggaactct acctggtttg cgaacaccaa agaattcgct
     4621 gacaacctgg acagcgattt taaagtacgt cagtacctga ctaaggaact ggctaaagcg
     4681 tccgtatctc gtatcgttat cgagcgtccg gctaagagca tccgtgtaac cattcacact
     4741 gctcgcccgg gtatcgttat cggtaaaaaa ggtgaagacg tagaaaaact gcgtaaggtc
     4801 gtagcggaca tcgctggcgt tcctgcacag atcaacatcg ccgaagttcg taagcctgaa
     4861 ctggacgcaa aactggttgc tgacagcatc acttctcagc tggaacgtcg cgttatgttc
     4921 cgtcgtgcta tgaagcgtgc tgtacagaac gcaatgcgtc tgggcgctaa aggtattaaa
     4981 gttgaagtta gcggccgtct gggcggcgcg gaaatcgcac gtaccgaatg gtaccgcgaa
     5041 ggtcgcgtac cgctgcacac tctgcgtgct gacatcgact acaacacctc tgaagcgcac
     5101 accacttacg gtgtaatcgg cgttaaagtg tggatcttca aaggcgagat cctgggtggt
     5161 atggctgctg ttgaacaacc ggaaaaaccg gctgctcagc ctaaaaagca gcagcgtaaa
     5221 ggccgtaaat aaggagcgtc gctgatgtta caaccaaagc gtacaaaatt ccgtaaaatg
     5281 cacaaaggcc gtaaccgcgg tctggcgcag ggtacggatg ttagcttcgg cagcttcggt
     5341 ctgaaagctg ttggccgtgg tcgtctgact gcccgtcaga tcgaagcagc acgtcgtgct
     5401 atgacccgtg cagttaagcg tcaaggtaag atctggatcc gtgtgttccc ggacaaaccg
     5461 atcactgaaa agccgctggc agtgcgtatg ggtaaaggta aaggtaacgt ggagtattgg
     5521 gttgccttga ttcagccggg taaagtcctg tatgaaatgg acggtgttcc ggaagagctg
     5581 gcccgtgaag cattcaagct ggcagcagcg aaactgccga ttaaaaccac ctttgtaact
     5641 aagacggtga tgtaatgaaa gcaaaagagc tgcgtgagaa gagcgttgaa gagctgaaca
     5701 ccgagctgct gaacctgctg cgtgagcagt tcaacctgcg tatgcaggct gcaagtggcc
     5761 agctgcaaca gtctcacctg ttgaagcaag tgcgtcgcga tgtcgcacgc gttaagactt
     5821 tactgaacga gaaggcgggt gcgtaatgac cgataaaatc cgtactctgc aaggtcgcgt
     5881 tgttagcgac aaaatggaga aatccattgt tgttgctatc gaacgttttg tgaaacaccc
     5941 gatctacggt aaattcatca agcgtacgac caaactgcac gtacatgacg agaacaacga
     6001 atgcggtatc ggtgacgtgg ttgaaatccg cgaatgccgt ccgctgtcca agactaaatc
     6061 ctggacgctg gttcgcgttg tagagaaagc ggttctgtaa tacagtacac tctctcgata
     6121 cgaataaacg gctcagaaat gagccgttta ttttttctac ccatatcctt gaagcggtgt
     6181 tataatgccg cgccctcgat atggggattt ttaacgacct gattttcggg tctcagtagt
     6241 agttgacatt agcggagcac taaaatgatc caagaacaga ctatgctgaa cgtcgccgac
     6301 aactccggtg cacgtcgcgt aatgtgtatc aaggttctgg gtggctcgca ccgtcgctac
     6361 gcaggcgtag gcgacatcat caagatcacc atcaaagaag caattccgcg tggtaaggtc
     6421 aaaaaaggtg atgtgctgaa ggcggtagtg gtgcgcacca agaagggtgt tcgtcgcccg
     6481 gacggttctg tcattcgctt cgatggtaat gcttgtgttc ttctgaacaa caacagcgag
     6541 cagcctatcg gtacgcgtat ttttgggccg gtaactcgtg agcttcgtag tgagaagttc
     6601 atgaaaatta tctctctggc accagaagta ctctaaggag cgaatcatgg cagcgaaaat
     6661 ccgtcgtgat gacgaagtta tcgtgttaac cggtaaagat aaaggtaaac gcggtaaagt
     6721 taagaatgtc ctgtcttccg gcaaggtcat tgttgaaggt atcaacctgg ttaagaaaca
     6781 tcagaagccg gttccggccc tgaaccaacc gggtggcatc gttgaaaaag aagccgctat
     6841 tcaggtttcc aacgtagcaa tcttcaatgc ggcaaccggc aaggctgacc gtgtaggctt
     6901 tagattcgaa gacggcaaaa aagtccgttt cttcaagtct aacagcgaaa ctatcaagta
     6961 atttggagta gtacgatggc gaaactgcat gattactaca aagacgaagt agttaaaaaa
     7021 ctcatgactg agtttaacta caattctgtc atgcaagtcc ctcgggtcga gaagatcacc
     7081 ctgaacatgg gtgttggtga agcgatcgct gacaaaaaac tgctggataa cgcagcagca
     7141 gacctggcag caatctccgg tcaaaaaccg ctgatcacca aagcacgcaa atctgttgca
     7201 ggcttcaaaa tccgtcaggg ctatccgatc ggctgtaaag taactctgcg tggcgaacgc
     7261 atgtgggagt tctttgagcg cctgatcact attgctgtac ctcgtattcg tgacttccgt
     7321 ggcctgtccg ctaagtcttt cgacggtcgt ggtaactaca gcatgggtgt ccgtgagcag
     7381 atcatcttcc cagaaatcga ctacgataaa gtcgaccgcg ttcgtggttt ggatattacc
     7441 attaccacta ctgcgaaatc tgacgaagaa ggccgcgctc tgctggctgc ctttgacttc
     7501 ccgttccgca agtaaggtag ggttactaaa tggctaagca atcaatgaaa gcacgcgaag
     7561 taaaacgcgt agctttagct gataaatact tcgcgaaacg cgctgaactg aaagcgatca
     7621 tctctgatgt gaacgcttcc gacgaagatc gttggaacgc tgttctcaag ctgcagactc
     7681 tgccgcgtga ttccagcccg tctcgtcagc gtaaccgctg ccgtcaaaca ggtcgcccgc
     7741 atggtttcct gcggaagttc gggttgagcc gtattaaggt ccgtgaagcc gctatgcgcg
     7801 gtgaaatccc gggtctgaaa aaggctagct ggtaattgtc accaattgaa tcacgggagt
     7861 aaagacagat gagcatgcaa gatccgatcg cggatatgct gacccgtatc cgtaacggtc
     7921 aggccgcgaa caaagctgcg gtcaccatgc cttcctccaa gctgaaagtg gcaatcgcca
     7981 acgtgctgaa ggaagaaggt tttattgaag attttaaagt tgaaggcgac accaagcctg
     8041 aactggaact tactctgaag tatttccagg gcaaagctgt tgtagaaagc attcagcgtg
     8101 tcagccgccc aggtctgcgc atctataaac gtaaagatga gctgccgaaa gttatggcgg
     8161 gtctgggtat cgcagttgtt tctacctcta aaggtgttat gactgatcgt gcagcgcgcc
     8221 aggctggtct tggtggcgaa attatctgct acgtagccta atcggaggaa aaaatgtctc
     8281 gtgttgctaa agcaccggtc gttgttcctg ccggcgttga cgtaaaaatc aacggtcagg
     8341 ttattacgat caaaggtaaa aacggcgagc tgactcgtac tctcaacgat gctgttgaag
     8401 ttaaacatgc agataatacc ctgaccttcg gtccgcgtga tggttacgca gacggttggg
     8461 cacaggctgg taccgcgcgt gccctgctga actcaatggt tatcggtgtt accgaaggct
     8521 tcactaagaa gctgcagctg gttggtgtag gttaccgtgc agcggttaaa ggcaatgtga
     8581 ttaacctgtc tctgggtttc tctcatcctg ttgaccatca gctgcctgcg ggtatcactg
     8641 ctgaatgtcc gactcagact gaaatcgtgc tgaaaggcgt tgataagcag gtgatcggcc
     8701 aggttgcagc ggatctgcgc gcctaccgtc gtcctgagcc ttataaaggc aagggtgttc
     8761 gttacgccga cgaagtcgtg cgtaccaaag aggctaagaa gaagtaaggt aacactatgg
     8821 ataagaaatt tgctcgtatc cgtcgtgcga cccgcgcacg ccgcaagctc caggagctgg
     8881 gcgcaactcg cctggtggta catcgtaccc cgcgtcacat ttacgcacag gtaattgcac
     8941 cgaacggttc tgaagttctg gtagctgctt ctactgtaga aaaagctatc gctgaacaac
     9001 tgaagtacac cggtaacaaa gacgcggctg cagctgtggg taaagctgtc gctgaacgcg
     9061 ctctggaaaa aggcatcaaa gatgtatcct ttgaccgttc cgggttccaa tatcatggtc
     9121 gtgtccaggc actggcagat gctgcccgtg aagctggcct tcagttctaa ggtagaggtg
     9181 taagatggct cacatcgaaa aacaagctgg cgaactgcag gaaaagctga tcgcggtaaa
     9241 ccgcgtatct aaaaccgtta aaggtggtcg tattttctcc ttcacagctc tgactgtagt
     9301 tggcgatggt aacggtcgcg ttggttttgg ttacggtaaa gcgcgtgaag ttccagcagc
     9361 gatccagaaa gcgatggaaa aagcccgtcg caatatgatt aacgtcgcgc tgaataacgg
     9421 cactctgcaa caccctgtta aaggtgttca cacgggttct cgcgtattca tgcagccggc
     9481 ttccgaaggt accggtatca tcgccggtgg tgcaatgcgc gccgttctgg aagtcgctgg
     9541 ggttcataac gttctggcta aagcatatgg ttccaccaac ccgatcaacg tggttcgtgc
     9601 aactattgat ggcctggaaa atatgaattc tccagaaatg gtcgctgcca agcgtggtaa
     9661 atccgttgaa gaaattctgg ggaaataaac catggcaaag actattaaaa ttactcaaac
     9721 ccgcagtgca atcggtcgtc tgccgaaaca caaggcaacg ctgcttggcc tgggtctgcg
     9781 tcgtattggt cacaccgtag agcgcgagga tactcctgct attcgcggta tgatcaacgc
     9841 ggtttccttc atggttaaag ttgaggagta agagatgcgt ttaaatactc tgtctccggc
     9901 cgaaggctcc aaaaaggcgg gtaaacgcct gggtcgtggt atcggttctg gcctcggtaa
     9961 aaccggtggt cgtggtcaca aaggtcagaa gtctcgttct ggcggtggcg tacgtcgcgg
    10021 tttcgagggt ggtcagatgc ctctgtaccg tcgtctgccg aaattcggct tcacttctcg
    10081 taaagcagcg attacagccg aagttcgtct gtctgacctg gctaaagtag aaggcggtgt
    10141 tgtagacctg aacacgctga aagcggctaa cattatcggt atccagatcg agttcgcgaa
    10201 agtgatcctg gctggcgaag taacgactcc ggtaactgtt cgtggcctgc gtgttactaa
    10261 aggcgctcgt gctgctatcg aagctgctgg cggtaaaatc gaggaataag tagcagatgg
    10321 ctaaacaacc gggattagat tttcaaagtg ccaaaggtgg cttaggcgag ctgaaacgca
    10381 gactgctgtt tgttatcggt gcgctgattg tgttccgtat tggctctttt attccgatcc
    10441 ctggtattga tgccgctgta cttgccaaac tgcttgagca acagcgaggc accatcattg
    10501 agatgtttaa catgttctct ggtggtgctc tcagccgtgc ttctatcttt gctctgggga
    10561 tcatgccgta tatttcggcg tcgatcatta tccagctgct gacggtggtt cacccaacgt
    10621 tggcggaaat taagaaagaa ggggagtctg gtcgtcgtaa gatcagccag tacacccgct
    10681 acggtactct ggtgctggca atattccagt cgatcggtat tgctactggt ctgccgaata
    10741 tgcctggtat gcaaggcctg gtgattaacc cgggctttgc attctacttc accgctgttg
    10801 taagtctggt cacaggaacg atgttcctga tgtggttggg cgaacagatt actgaacgag
    10861 gtatcggcaa cggtatttca atcattatct tcgtcggtat tgtcgcggga ctcccgccag
    10921 ccattgccca tactatcgag caagcgcgtc aaggcgacct gcacttcctc gtgttgctgt
    10981 tggttgcagt attagtattt gcagtgacgt tctttgttgt atttgttgag cgtggtcaac
    11041 gccgcattgt ggtaaactac gcgaaacgtc agcaaggtcg tcgtgtttat gctgcacaga
    11101 gcacacattt accgctgaaa gtgaatatgg cgggggtaat cccggcaatc ttcgcttcca
    11161 gtattattct gttcccggcg accatcgcgt catggttcgg gggcggtact ggttggaact
    11221 ggctgacaac aatttcgctg tatttgcagc ctgggcaacc gctttatgtg ttactctatg
    11281 cgtctgcaat catcttcttc tgtttcttct acacggcgtt ggttttcaac ccgcgtgaaa
    11341 cagcagataa cctgaagaag tccggtgcat ttgtaccagg aattcgtccg ggagagcaaa
    11401 cggcgaagta tatcgataaa gtaatgaccc gcctgaccct ggttggtgcg ctgtatatta
    11461 cctttatctg cctgatcccg gagttcatgc gtgatgcaat gaaagtaccg ttctacttcg
    11521 gtgggacctc actgcttatc gttgttgtcg tgattatgga ctttatggct caagtgcaaa
    11581 ctctgatgat gtccagtcag tatgagtctg cattgaagaa ggcgaacctg aaaggctacg
    11641 gccgataatt ggtcgcccga gaagttacgg agagtaaaaa tgaaagttcg tgcttccgtc
    11701 aagaaattat gccgtaactg caaaatcgtt aagcgtgatg gtgtcatccg tgtgatttgc
    11761 agtgccgagc cgaagcataa acagcgccaa ggctgatttt ttcgcatatt tttcttgcaa
    11821 agttgggttg agctggctag attagccagc caatcttttg tatgtctgtg cgtttccatt
    11881 tgagtatcct gaaaacgggc ttttcagcat ggaacgtaca tattaaatag taggagtgca
    11941 tagtggcccg tatagcaggc attaacattc ctgatcataa gcatgccgta atcgcattaa
    12001 cttcgattta tggcgtcggc aagacccgtt ctaaagccat cctggctgca gcgggtatcg
    12061 ctgaagatgt taagatcagt gagctgtctg aaggacaaat cgacacgctg cgtgacgaag
    12121 ttgccaaatt tgtcgttgaa ggtgatctgc gccgtgaaat cagcatgagc atcaagcgcc
    12181 tgatggatct tggttgctat cgcggtttgc gtcatcgtcg tggtctcccg gttcgcggtc
    12241 agcgtaccaa gaccaacgca cgtacccgta agggtccgcg caaaccgatc aagaaataat
    12301 cggggtgatt gaataatggc aaaggcacca attcgtgcac gtaaacgtgt aagaaaacaa
    12361 gtctctgacg gcgtggctca tatccatgct tctttcaaca acaccatcgt gactatcact
    12421 gatcgtcagg gtaacgcgtt gggttgggca acagccggtg gttccggttt ccgtggttct
    12481 cgcaaatcca ctccgtttgc agctcaggtt gcagcagagc gttgcgctga cgccgtgaaa
    12541 gaatacggca tcaagaatct ggaagttatg gttaaaggtc cgggtccagg ccgcgaatct
    12601 actattcgtg ctctgaacgc cgcaggtttc cgcatcacta acattactga tgtgactccg
    12661 atccctcata acggttgtcg tccgccgaaa aaacgtcgcg tataacgcct cgttttccag
    12721 gtttgttgga gaaagaaaat ggcaagatat ttgggtccta agctcaagct gagccgtcgt
    12781 gagggcaccg acttattcct taagtctggc gttcgcgcga tcgataccaa gtgtaaaatt
    12841 gaacaagctc ctggccagca cggtgcgcgt aaaccgcgtc tgtctgacta tggtgtgcag
    12901 ttgcgtgaaa agcaaaaagt tcgccgtatc tatggtgtgc tggagcgtca gttccgtaac
    12961 tactacaaag aagcagcacg tctgaaaggc aacaccggtg aaaacctgtt ggctctgctg
    13021 gaaggtcgtc tggacaacgt tgtataccgt atgggcttcg gtgccactcg tgcagaagca
    13081 cgtcagctgg ttagccataa agcaattatg gtaaacggtc gtgttgttaa catcgcttct
    13141 tatcaggtta gtccgaatga cgttgtaagc attcgtgaga aagcgaagaa gcagtctcgc
    13201 gtgaaagccg ctctggagct ggctgagcag cgtgaaaagc caacctggct ggaagttgat
    13261 gctggcaaga tggaaggtac gtttaagcgt aagccggagc gttctgatct gtctgcggac
    13321 attaacgaac acctgatcgt cgagctttac tccaagtaaa gcttagtacc aaagagagga
    13381 cacaatgcag ggttctgtga cagagtttct aaaaccgcgc ctggttgata tcgagcaagt
    13441 gagttcgacg cacgccaagg tgacccttga gcctttagag cgtggctttg gccatactct
    13501 gggtaacgca ctgcgccgta ttctgctctc atcgatgccg ggttgcgcgg tgaccgaggt
    13561 tgagattgat ggtgtactac atgagtacag caccaaagaa ggcgttcagg aagatatcct
    13621 ggaaatcctg ctcaacctga aagggctggc ggtgagagtt cagggcaaag atgaagttat
    13681 tcttaccttg aataaatctg gcattggccc tgtgactgca gccgatatca cccacgacgg
    13741 tgatgtcgaa atcgtcaagc cgcagcacgt gatctgccac ctgaccgatg agaacgcgtc
    13801 tattagcatg cgtatcaaag ttcagcgcgg tcgtggttat gtgccggctt ctacccgaat
    13861 tcattcggaa gaagatgagc gcccaatcgg ccgtctgctg gtcgacgcat gctacagccc
    13921 tgtggagcgt attgcctaca atgttgaagc agcgcgtgta gaacagcgta ccgacctgga
    13981 caagctggtc atcgaaatgg aaaccaacgg cacaatcgat cctgaagagg cgattcgtcg
    14041 tgcggcaacc attctggctg aacaactgga agctttcgtt gacttacgtg atgtacgtca
    14101 gcctgaagtg aaagaagaga aaccagagtt cgatccgatc ctgctgcgcc ctgttgacga
    14161 tctggaattg actgtccgct ctgctaactg ccttaaagca gaagctatcc actatatcgg
    14221 tgatctggta cagcgtaccg aggttgagct ccttaaaacg cctaaccttg gtaaaaaatc
    14281 tcttactgag attaaagacg tgctggcttc ccgtggactg tctctgggca tgcgcctgga
    14341 aaactggcca ccggcaagca tcgctgacga gtaaccggat cacaggttaa ggttttactg
    14401 agaaggataa ggtcatgcgc catcgtaaga gtggtcgtca actgaaccgc aacagcagcc
    14461 atcgccaggc tatgttccgc aatatggcag gttcactggt tcgtcatgaa atcatcaaga
    14521 cgactctgcc taaagcgaaa gagctgcgcc gcgtagttga gccgctgatt actcttgcca
    14581 agactgatag cgttgctaat cgtcgtctgg cattcgcccg tactcgtgat aacgagatcg
    14641 tggcaaaact gtttaacgaa ctgggcccgc gtttcgcgag ccgtgccggt ggttacactc
    14701 gtattctgaa gtgtggcttc cgtgcaggcg acaacgcgcc gatggcttac atcgagctgg
    14761 ttgatcgttc agagaaagca gaagctgctg cagagtaatc tgaagcaacg taaaaaaacc
    14821 cgccccggcg ggttttttta tacccgtagt atccccactt atctacaata gctgtactct
    14881 ttttgttcat cccctggagt atttatgtgg ttacttgacc agtgggcaga gcgccatata
    14941 gcagaagcgc aagcgaaagg tgagtttgat aacctggcag gtagcggcga accattgata
    15001 ctggatgatg attctcacgt gccaccggaa ttacgtgcgg ggtatcgctt gctgaagaat
    15061 gccggttgct taccgccaga acttgagcaa cggagagaag caattcagct tctggatatt
    15121 ctcaaaggta tccgtcacga tgatccgcaa tatcaagagg ttagccgtcg attgtcatta
    15181 ctggaattga agctgcgaca agctggattg agtaccgatt ttttacgcgg cgattatgct
    15241 gacaagttgt tggacaaaat caacgataac tagtggagta tgtatgtatc gcattggtga
    15301 gctggcaaaa atggcggaag taacacctga cacgattcgt tattacgaaa aacagcagat
    15361 gatggagcat gaagtgcgta ctgaaggcgg gtttcgcctg tataccgaaa gcgatctcca
    15421 gcgattgaaa tttatccgcc atgccagaca actaggtttc agtctggagt cgatccgcga
    15481 gttgctgtcg atccgcatcg atcctgaaca ccatacctgt caggagtcaa aaggcattgt
    15541 gcaggaaaga ttgcaggaag tcgaagcacg gatagccgag ttgcagagta tgcagcgttc
    15601 cttgcaacgc cttaacgatg cctgttgtgg gaccgcccat agcagtgttt attgctcgat
    15661 tcttgaagct cttgaacaag gggcgagtgg cgttaagagt ggctgttgat tttttgcact
    15721 gacaggatta cactcgcgcc gttaaataac caactggagt ttttatgagc cgatatcagc
    15781 atactaaagg gcagataaag gataatgcga tagaagcatt actacatgat cccttattcc
    15841 gacagcgagt agagaaaaat aagaaaggga aaggcagtta catgcgaaaa ggaaaacatg
    15901 gcaatcgggg gaactgggag gccagtggca agaaagtaaa tcactttttt accactggtc
    15961 ttctgctttc aggcgcttgt taagagcggt tattctgctc tttcagcaaa tcacgaattt
    16021 ctgccagtaa tacttcttct ttagttggtg caggtgcggc agctggttct tcttttttcc
    16081 gattcagttt gttgattagc ttaatcgcca taaagatggc aaaggccaca atcagaaaat
    16141 caaagacgtt ttgaatgaag acaccgtaat gcatcacaac agcagggata tccccctgcg
    16201 catcgcgtag cgtgacagca aactgtttaa agtcgatccc gccaattaat aagcccagag
    16261 gtggcatgat gatatcggca accagtgaag atacaatctt cccgaatgcc gcaccgataa
    16321 tgacacccac cgccaaatcc accacgttcc cgcgcatcgc aaattcgcga aattctttaa
    16381 taatgctcat gttattctcc ctatgaagcc gacaaccata agtctaacaa atgttaagcc
    16441 attttcctgc caggaatgaa tttaattaat ccttgataat caaagggaaa taataaggcg
    16501 tcattagacg ccttattaat tacaagaaga aagggcttgg ctggaagagt ctttcgacgt
    16561 cggtaataaa ctttttatct gtgaggaaca taattacgtg atcgccttgc tcaatgcgca
    16621 gattgtcatt ggcaatcatc acgtcgtttc cacgtaccac cgctccaata atcgttcctg
    16681 gcggtagctt gatttcgtca atgactctgc cgacaacgcg tgaggtgctt tcatcaccgt
    16741 gagcaacggc ttcaatagct tctgctacgc cgcggcgcaa tgaggaaaca ccaacaatat
    16801 ctgcttttcg cacatggcta agcaacgcag aaatagttgc ttgttgtggt gaaattgcaa
    16861 tatcgatcac gctcccctga accagatcca cataagcgcg acgctggatc aataccatca
    16921 cctttttcgc acccatacgt ttggcaagca tggcggacat gatattggcc tcgtcatcgt
    16981 tggtgacggc aataaacaga tcaacttgat cgatatgttc ttcggccagc agttcttgat
    17041 ccgacgcatc accaaaaaag acgatcgtat tctgcaactt ttccgccagt tcggcagcgc
    17101 gctgctgatt acgttcgatg agtttaacgc tgtaatcttt ttccagacga cgcgccagcc
    17161 ctgcaccgat attaccgcca ccaaccagca tgatccgctt atacggtttt tccagtcgct
    17221 gtaattcact catcaccgcg cggatatgct gtgaagcggc aataaagaac acttcatcac
    17281 cagcttcaac aatggtcgaa ccttgtggac gaatggggcg atcgtggcgg aaaattgctg
    17341 ccacacgagt atcgatatgt ggcatatgtt cgcgcatggt cgaaagtgca ttaccaatca
    17401 gcgggccgcc ataataggct ttaactacag cgaggctgac tttaccctca gcgaagttca
    17461 ccacctgcaa tgcgcctgga tactcaatca gtcggtaaat attatcgata accaactgct
    17521 ctggtgcgat cagatgatca atcggtacag catctgaatg aaatagctta tcggcatcgc
    17581 gtacgtagtc tggtgagcgg atgcgagcga tgcgattagg ggtgttgaaa agtgagtagg
    17641 ctacctggca ggcaaccata ttggtttcat ctgaactggt tacagcaacc agcatatcgg
    17701 cgtcgtcggc acctgcctcc cgcaatacgc gtggatgaga gccatgcccc tgcacgactc
    17761 gcaggtcaaa tttatcctgc aaggtccgca gacgctcacc gttggtatcg acaacagtaa
    17821 tatcgttgtt ctcgccaacc aggttttccg ccagtgtgcc gccaacctgg ccggcaccca
    17881 gaatgataat tttcatcagt ctcgacccgt tatctcatca ctttttgatt agcttagcgt
    17941 aaaagaagcc gtcgccctct tcggcaccag gcagattttg tttacccggt tgctctggtg
    18001 ttcctgtttc gcaaagttcg gcatcagcgg tacgttgcaa aaaagcttta atctgcaggc
    18061 tattctcttc cggtaacacc gaacaggtgg catagaccag ggttccaccg gattttaaat
    18121 gcggccaaat ggcgtcgaga atttcagact gcaattgcgc gagttccggg atatcgcgat
    18181 cgcgacgtaa ccatttaata tccggatggc gacgaatcac ccccgttgct gaacaaggcg
    18241 catctaataa aatgcgatca aactgttgct cgccacacca ttgggaaggg taacggccat
    18301 caccttgttt cacggtcgcc ttcataccaa ggcgttttaa attgtcgtga acgcgagaga
    18361 ggcgctgttc gtcaatatca accgcaacaa cctgcgcttc tggtgccacc tcaaggatat
    18421 gcgttgtttt accgccgggg gcggcacaaa gatccagaat gtgttcaccg ttttgtggcg
    18481 caagccaggt catgcaacct tgtgctgatg catcctgaac ggtaacccat ccgtcttcaa
    18541 aaccaggtag cgcatgaaca ggtgcaggtg tttccagccg tacagcatca gggtaatccg
    18601 catgcgggaa acctttcatt cctgcttcat ccaacaatgc aagccagctg tcgcgggaat
    18661 gatgcgtacg attaacgcgc agccacattg gcggacgctg gttattggct tcgacgatgg
    18721 attgccactg ctctggatac gctttttgca gacgcttcag caaccaggaa ggatgcagat
    18781 aacgtgcatc actggtatta aactcggcta ataactcttc ttgctgacgc tggaactggc
    18841 gtaatacacc gttaatcaac cctttaagtt gcggacgctt aattgcgata gcgccttcaa
    18901 ccgtttcagc cagcgcagca tgaggtggaa tgcgggtata aagcagttga tacaaaccaa
    18961 ccataatcag gtaatgcaca gtgcgctgtt tgccggtcat cggacgggcc attaacttat
    19021 taatcagcca gtctaactgc gaaagcgtac gcagtacgcc aaagcacaac tcttgaagaa
    19081 gtgctttgtc tttatcagaa actttttgct ggagcggtgg cagaatgttg cttaatgatt
    19141 gcccttgctc gacgacttgt tcaacggcct gggccgccat gctacgtaaa ttacgttgtt
    19201 ttttcataag tataaaaatg cccggtaaga ccgggcttag aagagtggac tatcaggcca
    19261 gacggttgcc cggaacaaac cattcccgac gagaattcag gaggtcttgc gcgctcatcg
    19321 ctttcttacc cgcaggttgt aacgagagca ggttcaggat gccatcacca gtcgcaacct
    19381 gaatgccttg tttgttggct tcaaggatcg ttcctggtgc agcgttggtt gccgtatcaa
    19441 tgaccgacgc tttccagact ttaaccggtt gtccttcaat ttccagccag ctcattggcc
    19501 atgggttgaa agcgcgaatg cagcgttcaa gctgcgctgc cgaaagcgac cagtcaatac
    19561 gcgcttcttc tttactcaac ttctcggcgt aagtgacaag agtttcgtcc tgaacttctg
    19621 gtttcgccgt gccgtctgct agttgtttca acgtggtgat aagcccttgt gggccaagct
    19681 ctgccagctt gtcgtacagc gtaccactgg tatcttctgc agtaatcggg caggagagct
    19741 tatagagcat atcaccggtg tctaaaccga catccatttg cataatggtc acaccagttt
    19801 ctgcatcacc cgcccatagt gagcgttgga ttggtgcagc accgcgccag cgtggcagca
    19861 gtgaaccatg aacgttgata cagccaagac gcggcatctc cagcactgct ttaggcagaa
    19921 ttaagccata ggcgacgacg accataacat cagcctgcag atcggcgacc agttgctggc
    19981 tttcttgtgg acgcagggaa acaggttgaa aaacgggcag acctttttcc tcagccagaa
    20041 ctttaaccgg gctgggcatc agttttttac cgcgtcctgc cggtcggtct ggctgggtga
    20101 acacgccaac gacgttatga ccagaagaca acagcgcgtc gagatgacgc gctgcaaagt
    20161 caggtgtacc cgcaaaaata atacgtagtg attctgacac gttagttctt atccttaagc
    20221 ccgggctttc agacgatcta gtttttcaac tttctgacga atacgttgtt gtttcagcgg
    20281 tgacagataa tccataaaca gtttgccgac caggtgatcc atctcatgct gaatacagat
    20341 ggctaacagc ccctctgctt ccagttcaaa tggtttaccg tcgcggtcaa gggcgcgaat
    20401 tttaactttc tctgcgcgcg gcactaaagc acgttgttca gggatcgaca ggcaaccttc
    20461 ttcaatgcct gtttcgccgc ttttttctaa aagctccgga ttgattaaca ccagccgttc
    20521 gtcacggttt tccgaaacat caataacaat gatacgttga tggatatcaa cctgggttgc
    20581 cgccaggcca ataccttctt ctgcgtacat cgtctcgaac atatcatcga cgatacgctg
    20641 tatttctgca ttcacttctt ctaccggttt agcaactttg cgaagccgct cgtccggaat
    20701 atgtaacact tgcaaaactg acataaatct ccagagatgt gttcaggggt tagaaagatt
    20761 atttcttcta ttctagacaa atccccctct gattgacagc atcactgacc aatcgcaaag
    20821 attgctaagg ctgcttatgg cagggagata aggatggtcg atacagaaat ttggctgcgt
    20881 ttaatgagta tcagcagctt gtacggcgat gatatggtcc gtatagctca ctggctggca
    20941 aaacagtcgc aaattgatgc ggttggattg cagcaaacag ggcttacatt gcggcaggca
    21001 caacgctttc tttcatttcc gcgaaagagt atcgaaagct cactttgttg gttggagcaa
    21061 cccaaccatc atttaatccc tgcggacagc gaattttatc ctcctcaact tctggtgacg
    21121 acagattacc ccggcgcact gtttgttgaa ggagaactgc acgcgctgca ttcatttcag
    21181 cttgccgtag tggggagtcg ggcgcattca tggtatggcg agcgatgggg acggttattt
    21241 tgcgaaactc tggcgacgcg tggagtgaca attacgagtg gactggcgcg tggaatcgat
    21301 ggtgtggcgc ataaagctgc cttacaggta aatggcgtca gcattgctgt attggggaat
    21361 ggacttaata ccattcatcc ccgccgccat gcccgactgg ctgccagtct gcttgaacat
    21421 ggcggagctc tcgtctcgga atttcccctc gatgttccac cccttgctta caatttccca
    21481 cgaagaaatc gcattatcag tggtctaagt aaaggtgtac tggtggtgga agcggctttg
    21541 cgcagtggtt cgctggtgac agcacgttgt gcgcttgagc agaggcgtga agtttttgcc
    21601 ttgccaggtc caatagggaa tccgggaagc gaagggcctc actggttaat aaaacaaggt
    21661 gcgattcttg tgacggaacc ggaagaaatt ctggaaaact tgcaatttgg attgcactgg
    21721 ttgccagacg cccctgaaaa ttcattttat tcaccagatc agcaagacgt ggcattgcca
    21781 tttcctgagc tcctggctaa cgtaggagat gaggtaacac ctgttgacgt cgtcgctgaa
    21841 cgtgccggcc aacctgtgcc agaggtagtt actcaactac tcgaactgga gttagcagga
    21901 tggatcgcag ctgtacccgg cggctatgtc cgattgagga gggcatgcca tgttcgacgt
    21961 actaatgtat ttgtttgaaa cctatattca cactgaagct gagttgcgtg tggatcaaga
    22021 caaacttgaa caggatctta ccgacgcagg ttttgagcga gaagatatct acaatgccct
    22081 gctatggctg gaaaaacttg ctgattatca ggaagggttg gcagaaccga tgcaactggc
    22141 ctcggatcct ctctccatgc gtatttatac accggaagag tgtgaacgac tggatgccag
    22201 ctgccgtggg tttctgcttt tccttgagca gattcaggtg ctcaaccttg aaactcgtga
    22261 aatggtgata gagcgagtgc tcgcgctgga taacgcagag ttcgaactgg atgatctgaa
    22321 atgggtgatc ctgatggtgt tgttcaacat cccggggtgc gaaaatgcgt accagcaaat
    22381 ggaagaatta ctctttgaag tgaatgaagg tatgctgcat taatcatttc tttaattcag
    22441 cataagttgt tatggcaaaa tcagcactgt tcacggtgcg taataatgag tcctgcccaa
    22501 agtgcggggc tgaactggtt attcgatccg ggaaacacgg tccgtttctt ggatgctcac
    22561 agtatccggc gtgtgactac gtccgtcttc tgaaatcttc agcggatgga catattgtca
    22621 aagttctgga ggggcaggtt tgccctgcat gtggcgcaaa tctggtatta cgccagggac
    22681 gctttggtat gtttattggt tgcagtaact accctgaatg cgaacatacc gaacttatcg
    22741 ataaaccgga cgaaacagca attacatgcc cccaatgtcg gacgggccat ctggtccagc
    22801 gccgctcccg ttatggcaaa acatttcact cctgtgatcg ctacccggag tgtcaatttg
    22861 ccattaactt caaaccaata gctggagaat gccctgagtg tcattatccg ctactcatcg
    22921 aaaagaaaac cgcgcagggt gtaaaacact tttgtgccag taaacaatgt ggaaagccgg
    22981 tttcggcgga ataataacgt gaataataac ctgcaaggag acgctatcgc agctgcgata
    23041 gatgtcctca atgaagaacg tgtcatcgcc tatccaacgg aagccgtttt cggtgttggg
    23101 tgcgatcctg atagcgaaac agcagtgatg agactgttgg agttaaaaca gcgtccggtt
    23161 gataaggggc tgattttaat cgcggcaaat tacgagcagc ttaaacccta tattgatgac
    23221 accatgctga ctgatgcgca gcgtgaaacc attttttccc gctggccagg tcctgtcacc
    23281 tttgtctttc ccgcgcctgc gacaacaccg cgctggttga cgggccgctt tgattcgctt
    23341 gctgtacgag tcaccgacca tccgttggtg gttgctttgt gccaggctta tggtaaaccg
    23401 ctggtttcta ccagtgccaa cttgagtgga ttgccacctt gtcgaacagt agacgaagtt
    23461 cgcgcacaat ttggcgcggc gttcccggtt gtgcctggtg aaacgggggg gcgtttaaat
    23521 ccttcagaaa tccgcgatgc cctgacgggt gaactgtttc gacaggggta acataatgga
    23581 aacctatgct gtttttggta atccgatagc ccacagcaaa tcgccattca ttcatcagca
    23641 atttgctcag caactgaata ttgaacatcc ctatgggcgc gtgttggcac ccatcaatga
    23701 tttcatcaac acactgaacg ctttctttcg tgctggtggt aaaggtgcga atgtgacggt
    23761 gccttttaaa gaagaggctt ttgccagagc ggatgagctt actgaacggg cagcgttggc
    23821 tggtgcagtt aataccctca agcggttaga agatggacgc ctgctgggtg acaataccga
    23881 tggtgtaggc ttgttaagcg atctggaacg tctgtctttt atccgccctg gtttacgtat
    23941 tctgcttatc ggcgctggtg gagcatctcg cggcgtacta ctgccacttc tttccctaga
    24001 ctgtgcggtg acaatcacta atcggacggt atcccgcgcg gaagagttgg ctaaattgtt
    24061 tgcgcacact ggcagtattc aggcgttggg tatggacgaa ctggaaggtc atgagtttga
    24121 tctcattatt aatgcaacat ccagtggcat cagtggtgat attccggcga tcccgtcatc
    24181 gctcattcat ccaggcattt attgctatga catgttctat cagaaaggaa aaactccttt
    24241 tctggcatgg tgtgagcagc gaggctcaaa gcgtaatgct gatggtttag gaatgctggt
    24301 ggcacaggcg gctcatgcct tccttctttg gcatggcgtt ctgcctgatg tagaaccggt
    24361 catcaaacta ttgcaacagg aattgtcagc gtgaatcagg ccatccagtt tccggatagg
    24421 gaagagtggg acgagaataa aagatgtgta tgttttcctg ctctcgtgaa tggtatgcaa
    24481 ctgacatgtg cgatctctgg cgagagtctg gcgtatcgct ttactggagg tacgccagaa
    24541 cagtggttag cgagttttcg tcagcatcgc tgggacctgg aagaagaagc ggaaaactta
    24601 attcaggaac aaagtgaaga tgatcaaggt tgggtctggt taccttgatc cagatattcg
    24661 tccttccatt tcacgtagtt atttgcggaa tagcgtaacc cagccttctc ttcatcactt
    24721 aacgggcgga tctgtttgac gggactaccg agatacagat atccgctctc cagccgttta
    24781 ttttgtggga ccaggctacc cgcaccaatc atcacatcat cttctactat tgcgccatca
    24841 agtaaaattg agcccatccc aaccaaaact cgattgccaa tggtgcagcc gtggagcatc
    24901 accttgtgac caacagtgac atcttcgcca atggttaatg ggttgccatc tgggttgtac
    24961 gaggatttat gagtgacatg caacatactg ccatcctgga tattggtgcg tgctccgatc
    25021 tgtacataat gtacatctcc acgaatcaca acgagcggcc agatccccac atcatcagcc
    25081 agacgaacgt caccaatcac gacgctgcta tcgtcgatca ttacgcgctg gccgatttgt
    25141 ggaaaaagat cgcggtatgg gcgtaaaaca tcagacatac tcacctcaac aataaatgat
    25201 ttcctaatga ctttgggtgc attattagcc ttgtgcaagt cttttagtat gcaaaaaagc
    25261 accgttttgt gtgcgaatgc agcaaaaagg gtgaaaaaac aacaaacaga aaaaaagatc
    25321 aaaaaagtac ttgtgcaaaa aattgggatc cctataatgc gcctccgttg agacgacaac
    25381 gtgaaacact tcacaggatg gtcggaacaa cgaagagaaa aaattctgaa attcagggtt
    25441 gactctgaaa gaggaaagcg taatatacgc cacctcgcga cagtgagctg aaagccgcgt
    25501 cgcaactgct ctttaacaat ttatcagaca atctgtgtgg gcactcgaag atacggattc
    25561 ttaacgtcgc aagacgaaaa atgaatacca agtctcaaga gtgaacacgt aattcattac
    25621 gaagtttaat tctttgagca tcaaactttt aaattgaaga gtttgatcat ggctcagatt
    25681 gaacgctggc ggcaggccta acacatgcaa gtcgaacggt aacaggaagc agcttgctgc
    25741 ttcgctgacg agtggcggac gggtgagtaa tgtctgggaa actgcctgat ggagggggat
    25801 aactactgga aacggtagct aataccgcat aatgtcgcaa gaccaaagag ggggaccttc
    25861 gggcctcttg ccatcggatg tgcccagatg ggattagctt gttggtgggg taacggctca
    25921 ccaaggcgac gatccctagc tggtctgaga ggatgaccag ccacactgga actgagacac
    25981 ggtccagact cctacgggag gcagcagtgg ggaatattgc acaatgggcg caagcctgat
    26041 gcagccatg
//
LOCUS       DAKISA010000054        25633 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.054, whole genome shotgun sequence.
ACCESSION   DAKISA010000054 DAKISA010000000
VERSION     DAKISA010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25633
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.054"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(64..795)
                     /gene="rlmB"
                     /locus_tag="OVW45_002366"
     CDS             complement(64..795)
                     /gene="rlmB"
                     /locus_tag="OVW45_002366"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCV5526796.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(975..3416)
                     /gene="rnr"
                     /locus_tag="OVW45_002367"
     CDS             complement(975..3416)
                     /gene="rnr"
                     /locus_tag="OVW45_002367"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCV5526797.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3455..3880)
                     /gene="nsrR"
                     /locus_tag="OVW45_002368"
     CDS             complement(3455..3880)
                     /gene="nsrR"
                     /locus_tag="OVW45_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCV5526798.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4085..5383)
                     /gene="purA"
                     /locus_tag="OVW45_002369"
     CDS             complement(4085..5383)
                     /gene="purA"
                     /locus_tag="OVW45_002369"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCV5526799.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5487..5684)
                     /locus_tag="OVW45_002370"
     CDS             complement(5487..5684)
                     /locus_tag="OVW45_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCV5526800.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5766..6770)
                     /gene="hflC"
                     /locus_tag="OVW45_002371"
     CDS             complement(5766..6770)
                     /gene="hflC"
                     /locus_tag="OVW45_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCV5526801.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6773..8032)
                     /gene="hflK"
                     /locus_tag="OVW45_002372"
     CDS             complement(6773..8032)
                     /gene="hflK"
                     /locus_tag="OVW45_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCV5526802.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8118..9398)
                     /gene="hflX"
                     /locus_tag="OVW45_002373"
     CDS             complement(8118..9398)
                     /gene="hflX"
                     /locus_tag="OVW45_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCV5526803.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9475..9783)
                     /gene="hfq"
                     /locus_tag="OVW45_002374"
     CDS             complement(9475..9783)
                     /gene="hfq"
                     /locus_tag="OVW45_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCV5526804.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9869..10819)
                     /gene="miaA"
                     /locus_tag="OVW45_002375"
     CDS             complement(9869..10819)
                     /gene="miaA"
                     /locus_tag="OVW45_002375"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCV5526805.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10812..12659)
                     /gene="mutL"
                     /locus_tag="OVW45_002376"
     CDS             complement(10812..12659)
                     /gene="mutL"
                     /locus_tag="OVW45_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCV5526806.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12669..14006)
                     /gene="amiB"
                     /locus_tag="OVW45_002377"
     CDS             complement(12669..14006)
                     /gene="amiB"
                     /locus_tag="OVW45_002377"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCV5526807.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14025..14486)
                     /gene="tsaE"
                     /locus_tag="OVW45_002378"
     CDS             complement(14025..14486)
                     /gene="tsaE"
                     /locus_tag="OVW45_002378"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCV5526808.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14458..16005)
                     /gene="nnr"
                     /locus_tag="OVW45_002379"
     CDS             complement(14458..16005)
                     /gene="nnr"
                     /locus_tag="OVW45_002379"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCV5526809.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16004..17143
                     /gene="queG"
                     /locus_tag="OVW45_002380"
     CDS             16004..17143
                     /gene="queG"
                     /locus_tag="OVW45_002380"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCV5526810.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCIACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAQ
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17126..17179)
                     /locus_tag="OVW45_002381"
     CDS             complement(17126..17179)
                     /locus_tag="OVW45_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5526811.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17413..17488)
                     /locus_tag="OVW45_002382"
     tRNA            complement(17413..17488)
                     /locus_tag="OVW45_002382"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17453..17455),aa:Gly,seq:gcc)
     gene            complement(17524..17599)
                     /locus_tag="OVW45_002383"
     tRNA            complement(17524..17599)
                     /locus_tag="OVW45_002383"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17564..17566),aa:Gly,seq:gcc)
     gene            complement(17757..17832)
                     /locus_tag="OVW45_002384"
     tRNA            complement(17757..17832)
                     /locus_tag="OVW45_002384"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17797..17799),aa:Gly,seq:gcc)
     gene            complement(18043..18588)
                     /gene="orn"
                     /locus_tag="OVW45_002385"
     CDS             complement(18043..18588)
                     /gene="orn"
                     /locus_tag="OVW45_002385"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCV5526812.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18683..19735
                     /gene="rsgA"
                     /locus_tag="OVW45_002386"
     CDS             18683..19735
                     /gene="rsgA"
                     /locus_tag="OVW45_002386"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCV5526813.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19832..20800
                     /gene="psd"
                     /locus_tag="OVW45_002387"
     CDS             19832..20800
                     /gene="psd"
                     /locus_tag="OVW45_002387"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCV5526814.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKICQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20822..24145
                     /gene="mscM"
                     /locus_tag="OVW45_002388"
     CDS             20822..24145
                     /gene="mscM"
                     /locus_tag="OVW45_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCV5526815.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDKPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24296..>25633)
                     /gene="yjeM"
                     /locus_tag="OVW45_002389"
     CDS             complement(24296..>25633)
                     /gene="yjeM"
                     /locus_tag="OVW45_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HCV5526816.1"
                     /translation="MMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWM
                     VSTSAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIA
                     RITAVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVF
                     AIFAYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLS
                     NGSVNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTL
                     CYSPLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAF
                     FNKLTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIAMVVVVLVVTF
                     ANVFTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
BASE COUNT         5926 a         7192 c         6501 g         6014 t
ORIGIN      
        1 tcttaccagc tatagacaaa aaaaaccatc caaatctgga tggcttttca taattcagac
       61 gaactagctg cgctgacgaa ccgcttcaaa taagcaaatt ccggttgcaa ccgaaacgtt
      121 cagggaagaa acacttcctg ccattgggat gctgatcaac tcatcgcaat gttcacgggt
      181 caggcgacgc ataccttcac cttccgcgcc catcaccagc gccaagcgtc cggtcatttt
      241 gctctgatag agtgtatgat ccgcctcgcc tgccgtaccg acgatccaga tattctcttc
      301 ctgcaacata cgcatggtgc gcgcaaggtt agtcacccga atcagtggaa cgctttctgc
      361 cgcgccgcag gctacttttt tcgccgtagc gttgagctgt gcggagcgat ctttcggcac
      421 aatcaccgcg tgaacgccag cagcgtccgc gctacgcagg cacgcgccga ggttgtgcgg
      481 atcggttaca ccgtcgagga tcagcaggaa cggttgatcg agcgaagcga tcagatccgg
      541 cagatcgttt tcctgatact gacgtcctgg cttcacgcgg gcgataatgc cctgatgcac
      601 ggcaccgtcg cttttctcgt cgagatattg gcggtttgcc aactggataa ccacgccctg
      661 agactcaagg gcgtgaatca gcggtaacag acgtttatct tcacggcctt ttaaaataaa
      721 gacttcctga aaacgttcag gggcgcgctc cagcagggcc tgcactgcgt ggatgccgta
      781 aatcatttcg ctcattaatg tactcgttgt ttacgttttg cctgatgcac tacgtttatc
      841 cggcctacat gatctctgca atatattgaa tttgcgtgat tatgtaggcc ggataaggcg
      901 ttcacgccgc atccggcatg aacaaagcgc attttgtcag caatctaacc ctcttctttt
      961 aaagagggta ttgatcactc tgccactttt ttcttcgccg cacgcttcgc tttggtcgct
     1021 gcggctattt tctgcgtttt cgccgatggc tttttcgctt ttctcgcgtc tttcttcgcc
     1081 gctttcggct tcgttttttt ctcaccgcgg aaggcgctgt ctggctcaaa gtttaccttt
     1141 ttaccgacct gacgacgctt gccgcctttt ttgcctgcat cgcctttttt cgctttctcg
     1201 cgcgccgttt taccgacgtt gcgcggtgcg cgttcactgg agatcagact aaagtcgatt
     1261 ttgcgctcgt ccatattaac cgcttcgacg cgaacttcca cgcgatcgcc caggcgataa
     1321 gtctggccgc tggattcccc catcaggcgt tgccctacct ggtcaaagcg atagtagtca
     1381 ttgtccagcg aagagacatg gaccagacca tcaatgaaca agtcgtccag acggacgaag
     1441 aagccaaagc cagtgacgct ggaaattacg cctttaaaga cgttacctac ctggtcgagc
     1501 atgaagtcac acttcagcca gtcagcgaca tcgcgcgttg cttcgtcggc acgacgttcc
     1561 gccatcgaac agtgctgacc cagttgcaac atctcttcca tcgaataatg gtagccgccg
     1621 gtttcagtgg tgttgccctg atgcccctgc tctttcgcca gcagatactt aatggcgcgg
     1681 tgcagcgtca ggtcaggata acgacgaatc ggcgaagtaa agtgcgcata ggactgcaat
     1741 gccaggccaa agtgaccacg gttttctgga tcgtaaatcg cctgtttcat cgagcgcagc
     1801 agcatggttt gcagcatttc tgcatcagga cggtcggcaa ccgactccag cagttccgcg
     1861 tagtcacgcg gttccggctt gttaccaccc ggcagctcca gccccagctc cgccagcact
     1921 gaacggaaag aggtaatcgc ttcggtgctc ggcttgtcgt gaatacggaa cagtgccggt
     1981 tctttcgctt tctcaacgaa acgcgccgcc gagatattcg ccagaatcat gcactcttca
     2041 attaatttgt gcgcgtcgtt acgctgggtc tgttcgatac gttcaatacg gcgttcagcg
     2101 ttgaaaatga acttcgcttc ttcgctctca aatgagatcc caccgcgttc ttcacgggct
     2161 ttatccagca ctttatagag gttatgcaac tcttcgagat gcttaaccag cggggcgtac
     2221 tgctcgcgca gatcctgatc gccctgcaga atatgccaga ctttggtgta ggtcagacgc
     2281 gcgtgagagc tcatcaccgc ttcgtagaat ttgtagcccg tcaggcggcc tttcgacgaa
     2341 accgtcatct cgcacaccat acacaggcgg tctacctgcg ggttgagcga acacaggccg
     2401 ttagagagca cttccggcag catcgggata acctgcgaag ggaagtacac cgacgtgcca
     2461 cggttacgcg cttctctgtc cagcggcgtt ggcggacgca catagtagct gacgtcggca
     2521 atcgcgaccc ataaacgcca gccgccgccg cgttttttct cgcagtaaac tgcatcgtca
     2581 aagtcacggg cgtcttcgcc atcaatggtg accagcggta aatcgcgcaa atcgacacgg
     2641 cccgcttttg cttcttccgg cacttcttct ttcagccccg caacctgttg ctcaacagcc
     2701 tgcggccaga tatacggaat ttcatgggta cgcagagcga tatcaaccgc catgccggtg
     2761 cccatattgt cgcccagcac ttcgacgatt ttacccaccg ctttggtgcg gcgagtcgga
     2821 cgctgagtca gttcgactac gaccacaaag cccatccgcg cgcccatgat ctgatcgggc
     2881 gggattaaga tatcgaagct cagacggcta tcgtcaggaa ccacaaagcc gacgcccgct
     2941 tcggtaaagt agcgaccaac aatctggctg gtttttggca ccagtacgcg gacaatacgc
     3001 gcttcacgac gacctttacg gtcagcaccc agcggctgcg ccagcacctg atcgccatga
     3061 atacaggttt tcatctgctc gctggagaga tacaaatcat ctttacgccc ttcaacccgc
     3121 agaaagccgt agccatcacg gtggccaata acggtacctt tcaccaggtc gaggcgttcc
     3181 ggcagcgcat agcactgacg acgagtgaag accagttgac catcgcgctc catcgcgcgc
     3241 aggcggcgac gcaggccttc aagctgctct tcgccttcaa tgtgcagttc taccgccagc
     3301 tcatcacggc tggccggttt ttcacgtttg gttaaatgtt cgaggataaa ttcccgacta
     3361 gggatgggat tcgcgtattt ttcagcttcg cgttcctgga aaggatcttg tgacatctcg
     3421 gttcctccgt tgtcatctct gatgaagatt ttcgtcactc caccagcaat aatttataaa
     3481 gcggttgatt ctcttcaacc aaatcggcaa gcgtgtagtt atccagttcc gtaagaaaac
     3541 tttgcacggc cttagaaagc gcctgtttca accgacaggc aggtgtaatg tggcaaaact
     3601 cactgctgca attcaccagc gataagggct ccagctcgcg caccacatca ccaatacgta
     3661 tcgcactcgc cggtttaccc aggcgaatgc cgccattttt tccacgaaca gcagtcacgt
     3721 agccggcacg actaagttga ttgattattt tgaccatatg attacgggag acgccgtaga
     3781 cgtcagtcac ttcagaaata ctggtcatcc gcccttctgg caatgacgcc atgtagatca
     3841 gcgcacgtaa tccgtaatca gtgaaactcg ttaactgcac atcaacctca aaagggaaat
     3901 cgggaaaaaa tacatttata ttgatgataa accagccaca agctgtgtcg ctaatttatt
     3961 tcagttcggg gaaggaaaaa agcgaggatt taacactgtg tcggatagcg taatggctta
     4021 accgacacag caatgtatca cactgttagc ccggcaggca aaatatctgc caggcgtacc
     4081 agaattacgc gtcgaacgga tcgcgcagaa tcatggtttc agtacgatcc ggaccggtag
     4141 agatgatatc gatcggcaca ccggtcagct cttcaatacg cttgatgtag ttcagcgccg
     4201 cctgcggcag gccgctacga tctttcacgc cgaaagtgga ttcagaccag cccggcatgg
     4261 tttcgtaaat cggctctaca cctttccagt cgtcagctgc cagcggagtg gtagtcactt
     4321 cgcgaccatc cggcatacgg taagccacgc agagtttaac ctctttcagg ccatccagaa
     4381 cgtccagctt ggtcaggcag aagccagaca gggagttcag ctgtaccgca cgacgaacgg
     4441 caacggtgtc cagccagccg gtacgacgac gacgacccgt agttgcgccg aattcgttac
     4501 cctgcttgca gaggaactcg ccagtttcat caaacagttc ggtcgggaac ggacctgcac
     4561 ctacacgagt tgagtaagct ttgaggatac ccagaacata atcaacataa cgcgggccca
     4621 ggccggaacc ggtcgccacg ccaccagcag tggtgttgga agaagttacg tacggataag
     4681 taccgtggtc gatatccagc agcgtaccct gcgcaccttc aaacatgacg aaatcgccac
     4741 gctgacgcgc ctggtcgagc aggtcagaaa cgtcaaccac catagaagtc aggatgtcgg
     4801 caacagccat cgtatcatcc agaactttct ggtaatcaac cgcttcagct ttgtagtagt
     4861 taaccaactg gaagttgtga tattccatca cttctttcag tttttcagcg aaggtttctt
     4921 tgtcgaaaag gtcgccaaca cgcagaccgc gacgtgctac tttatcttca taagcaggcc
     4981 cgataccacg accggtggtg ccgatcgctt tcgcgccacg cgctttctca cgcgcgttat
     5041 ccagcgcaac gtgataatca aggatcagcg gacatgcttc agacagcagc agacgctcac
     5101 gaacggggat gccacggtct tccagttctt tcatctcttt catcagcgcg gccggagaca
     5161 gcacaacacc gttaccgatg atgctggtta cattctcgcg gagaatacct gatggaataa
     5221 gatggagaac ggttttttca ccgttgatta cgagagtatg gcctgcgttg tgaccgccct
     5281 ggtagcgtac aacatattta gcccgttcag tcagaagatc gacgatctta cctttacctt
     5341 cgtcacccca ttgggtgccc agtacgacga cgttgttacc catttttcaa aatcaccgtt
     5401 tgcttaaaaa tggattctac catcgctttt tcagagttac agcacttttt gcactcaaaa
     5461 atgacctcga tcagtctgtt ttttgttcag ccaatcgttt tcctcaacat gtagtagacc
     5521 acaacgcccg caaccacaag tccaccgcca aaacgacgta aaatattatc gggcaaattg
     5581 gtcatcgcag agatcatctt cttccatgcc ttcgggtaaa gcatcggccc taaaccttcc
     5641 agtaccaaaa ccagggcaag cgccagccag attgtcgaat tcattcatta tccttataga
     5701 aaaagaaaac caccgacatc cctgaggatg cagtggcttt attgacctgt accgcagtcg
     5761 ttatattaac gcgttgcgga agtcggcgtc ttcatgtagc ggaagaaatc gctatccggg
     5821 ctcatgacca tcacgtcctg attgccagag aagctgttct cataagcacg caggctacgg
     5881 atgaatgcgt agaagtccgg atctttactg aatgcatcag caaacagttt ggctgcttcg
     5941 gcatcgcctt caccacgcat gatgcggccc tgacgctcag cttctgccag cgttctggtc
     6001 acttcatagt cggcagtcgc gcgcagtttt tccgcttctt cctgaccttg tgaacggtga
     6061 cgacgcgcta ccgcttcacg ctcggcgcgc atacggttgt agatcgcttc agacacttcg
     6121 gtcggcaggt tgatctgctt gatacgcaca tcgacaactt caatacccag cgccgccata
     6181 ctgttcgggt tgatgaccgg aactttgccc ttcgtctcag ccgttacgcg ctctgccgct
     6241 tcggcaatgg cgttatctgc cgccggggta gtaacttcat cttctgtacc cgcagaaccg
     6301 gagttcagcg cgtcacgtac ttcgagggtc agacgaccac gggaatcggt gacgatgtct
     6361 ttcacgtcca ggcgaccaat ttcagaacgc agacggtcag agaacttacg tttcaacagc
     6421 acttccgctt gcgaaatgtc gccaccaccc gttgccaggt agtaacggct gaaatcgctg
     6481 atgcgccatt tgatgtaaga atcgacgatc aggtctttct tctctttggt cacaaagcgg
     6541 tcggcctggt tgtccatggt ctgaatacgt gcgtcgagca ttttcaccgt ttcaatgaac
     6601 ggtatcttga aatgcagacc cggctcataa accagaggtt tgttgtcatc gtcacgcagt
     6661 accttaccaa aacgcagcgt aataccgcgc tcaccttctt tgacgacaaa gacagacatg
     6721 taaagcacta ccagcacgat gatgataatc gcgataactg acttacgcat cgttattccc
     6781 cctgacgctg gtagtcgtta cgctgcgcgt tggcgcggcg ttggtccata atatcgccct
     6841 gactggtgga cgacgtgttg cttgctccac tggttgtgga ggaagaggct ggcggcagac
     6901 gcagcagatt gctggcaccg ttatcgctct tcgccgcagg ggcgttaccg cctttcagca
     6961 tctggtctaa cggcagaacc atcaggttgc cacctttatc gttaaccagc actttgcggg
     7021 tgttacccaa cactttttcc atcgtctcga tatacagacg ctcgcgagta atttccggcg
     7081 cggctttata ttccggcaga agtttagcaa agcgcgccac ttcaccctga gcttccagga
     7141 tggtctgggc cttgtacgca cgcgcctctt cgaggatacg ttgcgcctga ccgttcgcac
     7201 gcggctgaac ttcgttggta tacgcttctg cttcacgaat gtattgctgt tcgttttcac
     7261 gcgcggcaat cgcatcgtca aacgccgctt ttacttcttc cggcggacga gctgcctgga
     7321 agttgacgtc cagcagagtg atacccatgt catacggacg gatagtctct tccagttcgc
     7381 gctgagtatc gctacgaatc acggtacgac cttccgtcag aatgcggtcc atggtgtatt
     7441 taccgataac tccacgcagg gcgctgtcgg tagcctgacg caggctgtca tccgggctgg
     7501 tcacgctata cagatatttt tccggattgg tgacgcggta ctgcacgttc atctcaacgc
     7561 gcactacgtt ctcgtccgac gtcagcatca caccagaagc ggccagttca cgcacggctt
     7621 ccacgttcac cggtttgact tcgtcgataa acgtcggttt ccagttcaga cccggctcaa
     7681 ccagatggct gaatttacca aagcgtgtta ccacgccgcg ttcggcttct ttaatggtat
     7741 agaaaccact ggccgcccag ataatgacaa tcgctgccgc tgcgatggta acgacacgac
     7801 cgccaagctg cgggcgcggg ccttgcgatg aactgccacc gccagatccg gtgcctttac
     7861 cgccgcccag accaccgagc tttttgctca gtttgcggaa gatatcatct aaatcaggtg
     7921 gcccttgatc gcgaccgcct ttgtttccat ttccctcaga gttgccgcca ggtttgctgc
     7981 ttccccacgg gtcgcggtct tgtccgttat taccgggctg attccacgcc atgtttgtgc
     8041 tccatatttg ttatgcggtg atccctgttg tctttcgacg ccagaggata tgactccacg
     8101 cttcagacgc tacgccgtta gatcaggtaa tcgatcaacg ccggttcttg tttacagagg
     8161 cgacgccagt caacgatcgg catacgaact tgcagactta cgctgccgtc ctcctccatc
     8221 cactcttttt ctattgcctg aagctgataa aaacgacttc tcagacgtcc ttcctgcggc
     8281 ggcaaacgca atgtatgctg cgccacctcg ccggaaagcc gctccgtcaa agcctgaaaa
     8341 agctgtggta tccccgctcc ggtctgtgcg gaaagccaga cacggatcgg tttgttctct
     8401 tcgtcccgat caatacgcgg ttcgaaatct tccagcatat cgatcttgtt catcaccagc
     8461 agggttggga tctcgtgagc gtcgatctct tcaagaaccg tattcaccgc ttcgatgttt
     8521 tcttgtacac gtacatccgc cgcatcaatg acgtgcagca gtaatgtggc ttgccgcgtc
     8581 tcttgtaacg tggctttaaa tgctgccacc agatcgtgcg gcaggtggcg aataaaccct
     8641 acggtatctg caagtacggt ttcaccgaca tccgcgacgt caatacgccg caacgtcggg
     8701 tcgagggtgg caaacaactg gtctgccgcg tagacccgcg cttcggtgat gcgattgaaa
     8761 agggtagatt taccggcgtt ggtatatccc accagcgaaa cagtaggaac gtcggctttg
     8821 atacgcgatt gccgcccctg ctcacgctgc ttttcaactc tttctaggcg cgactgtatc
     8881 tgcacgatgc gattacgcaa caaacgacgg tcggtttcga gctgggtttc acccggacca
     8941 cgcaaaccta tcccgccttt ctgtctttca aggtgggtcc agccacgcac caggcgcgta
     9001 gccagatggc gcagctgcgc cagctcaacc tgcaacttac cctcatgggt acgcgcacgt
     9061 tgggcgaaaa tatctaaaat aaggccggtg cggtcgataa cacgacactc gcacaaacgc
     9121 tccaggttac gctcttgcgc cgggctcagg gcatggtcaa aaagaacgac cgaagcaccc
     9181 gtcgctttga cagcttccgc aatttcaact gctttacctt cacctacaaa atactttggg
     9241 tgcggcgctt tacggctacc ggtaatcacc tgcaatgctt cgacaccggc ggaagagacc
     9301 agagattcaa actcctggag gtcttccata tctttgtctt gcgtaaaata gatgtgtacc
     9361 agtaccgcct gctcaccagc atcataacgg tcaaacaagc gtataaccct ctaaatagat
     9421 cagcggggaa cgcaggatcg ctggctcccc gtgtaaaaaa aacagcccga aaccttattc
     9481 ggtttcttcg ctgtcctgtt gcgcggaagt attctgcgcg ctgctaccat gatggtagtt
     9541 actgctggta ccgccaccgg cgttgttact gtgatgagaa accgggcgag acgggacaac
     9601 agtagaaatc gcgtgcttgt aaaccatctg gctgaccgtg tttttcaaca ggatcacgaa
     9661 ctgatcaaaa gactcgattt gcccttgcag cttaatacca ttcaccaaat aaatagaaac
     9721 tggaacacgt tcccgacgca gtgcgttcag gaacggatct tgtaaagatt gccccttagc
     9781 cattctctct tttccttata tgcttatttg tactttgaac ctttcgattc tgaaaaaatt
     9841 gcgcacgata cgtctcaatt gtacacattc agcctgcgat agcaccaaca acctgtaata
     9901 cttcgtcacg cgcctgttct ggtttttcac tgtcaagcca gtgaacccct tcccaaccac
     9961 gcagccaggt tatctgccgc ttcgccaact gtctcgtggc gcaaacacct cgataaacca
    10021 tttcatcgta tgagatttcg ccttcaaggt aagaccacat ctggcgataa cccacgcaac
    10081 gaatggaagg caagtccgta tgcaaatctc ctcgggcaaa aagcgcccgg acttctgctt
    10141 caaaacctga agccaacatc tgatgaaaac gctgctcaat gcgttgatgg agcagttcac
    10201 ggctcgccgg ggcgatggcg aactgatgca cctgatacgg tagagcgtct cctgacgttt
    10261 gcgtcagttc cgttaaagtt ttacccgaaa tgaaaaaaac ttccagtgcc cgggaaagcc
    10321 tttgtggatc atttggatga atccttgctg ccgcaaccgg atctacctcc tgaagttgac
    10381 gatgcaatga ctcccaacct tgctctgccg cctgttgctc aattctggcc cgcacttccg
    10441 ggtctgccga cggtagcggc gacaaccctt ccagcaatgc cttgaaatac aacatcgtac
    10501 cgcccactaa cagtgggatc cgccccgccg cggtgatatc ggccatttcc gccagcgcat
    10561 cgcggcgaaa atcagcagcc gagtaagcct gcgacggatc gcgaatatcc agcaatcggt
    10621 gcggcgcggc gagtaactct tcagcgttcg gcttcgccgt cccgatatcc atccctttgt
    10681 aaataagggc agaatcaacg cttatcaact ctactggtaa aattttacgc agctcaatgg
    10741 ctaacgccgt tttaccggag gccgtcggcc ccatcaaaaa aatcgcctta ggcaggctcg
    10801 ccttactgat atcactcatc tttcagggct tttatcgccg gatgtaaatc aacagattgt
    10861 aacagaccac ccggcggcgt tttcacaagt tgcggacata accgttccac gtccgccagc
    10921 agggttatgg cctgtgccat tgaccactgc gcatgttcgc tcatcagatt tcgtgcaatc
    10981 cactgcgcaa tattgccagg ttcgaatacg gactgcttcg ccaggtagcc tatcagttca
    11041 ggaatcaaga tttgtaaatt ttgttggcgt aagggtaaag gcacggccct gatggtcaca
    11101 tgctgtgcat ctgactggaa atcaataccc aattccgcca gggcagactg cgctttttct
    11161 aatgccgatt tttcttcgcc agaaactttt agccgcaacg gaataagcag cggctgagcg
    11221 caaactggcg cttcacccgg cgtcagttgt acctgacgca gccaacgttc tgccactggc
    11281 aaggctaaaa gtgaaatgtt gccgtcgcgc tccagcaacg cacagtcgga atggacgata
    11341 gtcagtaccc gaccaaaact ctgactgttc gccgcaagtg caggttcctg cggttccggc
    11401 gcttttaatt tttgcatcgg cgcgggcgtt tgcaaaagct ggcgatacac ttcaccttgc
    11461 tgtttctggt agcctggctg cgcattcggc cagggggcag ccggacgact gcctgatgct
    11521 ggcgcaggag tgtagcgcgg agctaccggc tcacgagctg ccggttctgc aaagtgattg
    11581 cgccccgccg ccacgcggtt ttccggaatg gaacgcggtg caggttgggg ttcatcgtcc
    11641 agcggtagcg gcgtttccag ttgctgttgt agcacgctca gcacgccctg atagataaaa
    11701 tcatgcacca gacgcgactg atggaaacgc acttcgtgtt tggcggggtg cacgttgacg
    11761 tccacctgat gcggatcgat ctccagatac agcacaaatg ccggttgctg atcggccccc
    11821 agtttgtctt cgcaggcctg gcggatcgcg tgattgatca ggcgatcgcg catcatgcga
    11881 ccgttcacgt agcaatactg aatttctgcc agtgcgggcg tggtgtgatt tggatcggcc
    11941 acccagccgc gtagcgtgag atcgccgtgt tgccattcaa tcgccagcgc ttgttcaaga
    12001 aaagcggtgc cgcaaatcgc gcctaagcgc cgttcttttt gcccgccttc cggcactgcg
    12061 cggtactgac gcacaatttt accgttatgc gacaggttga tcgtgacgtc gaaacgcgcc
    12121 agcgcaatgc ggcggatgat ctcatcaatg tggttaaatt cggttttctc ggtgcgcagg
    12181 aatttgcgcc gcgccggggt gttgtagaac agatccagca cctccagcgt cgtccccaca
    12241 ggatgcgccg ccggttttac cgtcacgttc atatcgcgcc cttcggcata ggcctgccag
    12301 gcttcctgct gttctgcggt gcgtgaagtg agcgtcaggc gggaaaccga actgatactc
    12361 gccagcgcct caccgcgaaa gcccaggctg ataatggctt cgagatcgtc cagagaggcg
    12421 attttactgg tggcatgacg agccagcgcc agcgccagct catctttttt gataccgcag
    12481 ccgttatcac gaatgcggat aagtttcgcc ccaccgcgtt cgatatcaat atcgatacgc
    12541 gtcgcacctg catcgaggct gttttccacc agttctttga ctaccgacgc aggtcgctcg
    12601 accacctcac ctgcggcaat ctggttcgcc agttgtggcg gtaagacctg aattggcatc
    12661 aatcgtcctt agtttggcag cgtgcgatct ggcgtcgtca ccgtactggc agtttgtgcc
    12721 gttgccccct gcggcgcaga ttgcatcgga tgcgcaagga aataattgcg caggcctttg
    12781 taaatggctt ctgccagctg ttgttggtaa tcgtcgctcg ccagcaaacg ttcttcgctg
    12841 ttgttgctga taaaaccggt ttcgaccagt actgacggga tatccggcga acgcagaacg
    12901 ccgagactcg cgtgttctgg tcggcgttta tgtatttcgc caatgcgttg caactgactg
    12961 atcatactgg tcgctacatc ataccctacc cgctgggaat gaccgaactg taaatccagc
    13021 accgcctggc ttaaataggg gtcagactga ctgttcgcca gcacatcgcc cgccccaccc
    13081 agcagctccg actgtttctc gtgctgctcc agccagctgg ccatttcgct gttggcgcga
    13141 cggttagaga gcacccatac ggaagcgcca gtcgcactgc ggttcggtgc ggcatcagcg
    13201 tgaatcgaca cgaggaaatt ggcgttttgc ttacgtgcca catcgctgcg ccccatcacc
    13261 gagataaagt aatccccgtc acgggttaaa acgcctttaa acatcggatc gtcattgagc
    13321 aaagtgcgca atttacgcgc gatggcgatg gtgacatttt tctcccgcgt accaccgggg
    13381 ccgatagcgc cagggtcctg accgccgtgt ccggcatcaa tagcgatgat aattttatcg
    13441 ccagtgttag ccgtcgcgcg cgctgccgga cgcgttaccg tattactgct gataacaccc
    13501 gtagtgcggt tactttccgt tttaaacgga ttgcgcgccg gttcgctgac gcgcggtgcg
    13561 acaaccgcag gcgtttcaac gcgtttcgca accacgggcg gtggaggagg cggtggcggc
    13621 acatcggcgt taatcgtaaa gacgacagtg taattgctgc cattctgccg cttcaccgct
    13681 tcggttttac cgttttcggt aagatcgacc accagccgta gcgtttgtgc atctttaggc
    13741 gttccagagc gaatcgcctt caccagatta ttgccgctga acaacaacgg cagtccctga
    13801 atcacgcccg tttgtttgat atcgagcgcc acggtgcgtt tgctttgatg gctaaacgca
    13861 taatcaggat cgccaataaa actcaacgtt atccgcgcct gttgattacc gttagaaacc
    13921 tgaatatcag agagcgtcgc ggcacccacc ggcgtgcaca gcagcagcag cgtcgctacc
    13981 aaccaatttc tgatgcgata catcatcccg ccacctttca aaggttaacc ggctaaacgc
    14041 gccagcaaca attcacccgc agaggaaacc gcactcacgc gcgcctcacg gccttgtgcc
    14101 tgataatcaa tgtgtatttc gacatccggg tcaggaagaa cacctgtacc ttgttgtggc
    14161 cactccacca ggcagatggc atcgttggca aaataatcgc ggatccccat aaactccagc
    14221 tcctcgggat cggcaaggcg gtacaaatca aagtgataaa ccattaaatt gtcgagcgta
    14281 tagggttcga ccagcgtata agtggggctt ttgacattac cctgatgacc cagagcttgt
    14341 aaaaaacccc ggctaaaggt ggttttacct gcgcctaaat cgccatacag atagattacg
    14401 gttgcgccat cgcaggcttt cgctacccgc tcgcccaggt ctaatgttgc ctgctcatca
    14461 gggagcggaa ttactcgatt catcatggtt tttatcagtc acttccgggt taacaatacg
    14521 ctgtagcgtg gaaaagagat cggttgccag catcccgcgc gttccaaaac gcgccgccag
    14581 tacgtcagct gccgcaccgt gcgcgacaca gcccgcacag gcggcatcat acggcgacag
    14641 tttttgccca agcaatgcgc caataatacc agagagcaca tcgcccatgc cgccgctcgc
    14701 catgcctgca tttccggcat caataatgcc taaagcgtca ggatgggcgg cgaccacggt
    14761 tccggcacct ttcagcaccg ctacgccgcc ataacgttgt accagacgtt tggcgcaatg
    14821 taagcggtca ctttcaattt cagcgacgga acagcctaac aaccgtgcgg cctcgccagg
    14881 atgcggcgtg atcacgcgat tgtgacgctt atcgggattg attgccagca ggttcaatgc
    14941 atcggcatcc cacaacatcg gtttgcgaaa attctcaact ttttgcagtg cttttttccc
    15001 ccactcttgc tggcccagac cgggaccaat caccaccaca tcggcccatt ccaggctttc
    15061 ggtaagagag tccatcgtca gttcatgcac catcaattcc ggtcgtgcag tcagcagcgg
    15121 cgcaatgttt tcactgcggg tcagtactcg gactaaacca gcaccagcac gcagcgccgc
    15181 ttcccccgtc atacgaatag cccccgccgt gccgtgatcg ccaccgataa tcaccagccg
    15241 cccgtgatcg cctttatgcg aagtcgggcg acgcggtttc agccagtgag aaagttgttc
    15301 tgccgaaaac cgctgaattt tcgtctcctg acctgccagc cagctatcca gccccagtga
    15361 gtcaaaatgc agttgtcctg taacatcccg cgcttttcca gtgagcaagc ctggtttcag
    15421 cgcaataaaa gtgatggtgt gatcggcgtt gatcactgcg cctggcgtag cgccggtttc
    15481 agccagcagg ccggaaggga tatcaaccgc cacaatcggc gcaggatggg aattagcgtg
    15541 gtcgattaac tggctaatgg attcgcgggg cgcttgctgc aagccggtac cgagcagcgc
    15601 atcaacaatc agatctaccg attcgggcca gacaatattc gaagcatgga tctcgccacc
    15661 cgcgtttaac catgcttcgc gtgccagcgc ggcctcttcc ggcaacggtt tgtcgctctc
    15721 ctgggccaac aacgtgacct caatgccgac cgctttggcc agtcgcgcga ccacgtagcc
    15781 atcgccgccg ttattaccat gaccgcacag caccagccag tggcgggcgt caggatacgc
    15841 cgaacgacac acctggaatg cggcctcgcc agcgcgaagc atcagctcat agagtgtgag
    15901 ccccagcaca tctgccgcct cgcgttctcc gcggcggata tcgtcggcgt accagacggt
    15961 gtgtggtata cttacggggt ttttcttcat tgtatggtcc gtcatgtcag agcccctcga
    16021 tctcaatcag ttagcgcaaa aaattaaaca gtgggggctg gaactgggct ttcagcaggt
    16081 aggtattacc gataccgatc tcagcgagtc cgagcccaaa ctgcaagcat ggctggacaa
    16141 acaataccac ggcgaaatgg actggatggc acgtcacggt atgctgcgcg ctcgccccca
    16201 tgagttatta ccaggtacgc tgcgtgtaat cagcgtgcgg atgaattacc ttcctgctaa
    16261 cgccgcattt gccagcacgc tgaaaaaccc caaactcggc tatgttagcc gttatgcgct
    16321 gggccgtgac tatcacaaac ttctgcgcaa ccgactcaaa aagctgggcg agatgattca
    16381 gcaacattgt gtttcgctga attttagacc gtttgtcgat tctgcgccta ttctcgagcg
    16441 cccgttagct gaaaaagctg ggctcggctg gacaggtaag cactcactta tcctcaatcg
    16501 cgaggccggt tctttcttct ttttaggcga attgctggtc gatattccgc tgcccgtgga
    16561 tcaaccagtc gaggaaggat gcggtaaatg catagcctgt atgacgattt gcccgaccgg
    16621 tgccatcgtc gagccatata ccgtcgatgc tcgccgctgt atctcttatc tcaccatcga
    16681 acttgaaggg gcgatcccgg aagagttgcg accgttaatg ggaaaccgta tttacggttg
    16741 cgatgactgc cagcttatct gcccgtggaa tcgctattcg caactcacta cagaagacga
    16801 tttcagcccg cgtaagccgc tacacgcaca ggaactcatt gagttatttg cctggagcga
    16861 agagaagttt ttaaaagtca cggaaggatc ggcgattcgc cgtataggtc acctgcgctg
    16921 gctgcgtaat atcgccgtgg cattaggcaa tgcgccctgg gatgaaacga ttctggcggc
    16981 gctggagagt cgcaaaggtg agcacccact tcttgatgag cacatagcgt gggcgattgc
    17041 gcagcaaatc gagagacgaa atgcgtgcat agtcgaagtg caattgccga aaaaacagcg
    17101 tctggttcgg gtgattgaaa aagggttacc gcgtgacgcc tgattcatcc acagcttgtg
    17161 aataaaaata aaaacgcatt gcaattcaag acgcataaaa tcgtcaagtg atcgactcga
    17221 caaaacggaa tcttgatttt taacttattt atcaataggt taaaaaaata ccattcaccg
    17281 tgcgaagttt ctttgaaatt aagattacag cctgaggatg tggataactc tgtttacaag
    17341 agtttgtcag agacaacaaa aaccagcaat aacgcatcgc gtcgctgtgg atattttatt
    17401 gagagaagaa tttggagcgg gaaacgagac tcgaactcgc gaccccgacc ttggcaaggt
    17461 cgtgctctac caactgagct attcccgctt gggtggtctg tgccttacag cactttcaaa
    17521 ttttggagcg ggaaacgaga ctcgaactcg cgaccccgac cttggcaagg tcgtgctcta
    17581 ccaactgagc tattcccgct taatcttcgt ctttcgaacc gctactgcct tggctgcctt
    17641 cactcacctt agtcacatac ttttgtaagt tcccaaggat tcgttcagtt gccgccttgt
    17701 cgcaattcga aatactgcga ttgggtggtc tgtgctttgc agcacttttc aaattttgga
    17761 gcgggaaacg agactcgaac tcgcgacccc gaccttggca aggtcgtgct ctaccaactg
    17821 agctattccc gcgtactact taattttgct ttcgtaattt tcgcatttct gcgtcgttac
    17881 gggaggcgca ttatacgaga aatctttccc tctgcaaccc cccaaaaaag ttttttttgc
    17941 gtttttatct caagtgctga aattatcagc aagacgttca atttagcgtc aaaatcacgc
    18001 cgccgcaagt ggattccggc gcttacgtga ccaggaaaaa ttttacagct tgataaaatg
    18061 ctcgcggtag taagccagct ccgccaccga ttcacggata tcatccattg cctgatgcgt
    18121 cccctgcttg gtaaaaccat ccagaatttc cggcttccag cggcgcgcca gctctttcag
    18181 ggtgctgaca tcgagataac ggtagtggaa gtaggcttcc agctccggca tgtatttaaa
    18241 caggaaacga cggtcctgtc cgatgctgtt accgcaaatc ggcgattttc ccgcaggcac
    18301 ccactgtttt aagaattcga gcgttgccag ttcagcttcc cgatcgccca tcgtgctcgc
    18361 tttcacgcgc tctaccagcc cgctggcggt atgggtgcgc acgttccagt catccatcag
    18421 cgccagctgt tcatcggact gatgtactgc aatggtcggc ccttctgcca gaatattcag
    18481 attggcatcg gtcaccagcg tggcaatctc aataatgcga tcgcgctcgg gatccagacc
    18541 ggtcatctca agatcgatcc aaatcaggtt gttttcattg gcactcatgc tattttccac
    18601 ccattcacag tgactatatt gatccacaat tgcgtgtatc atagatgttt tgcccatcag
    18661 gggcgaccag gagtcagtac gattgagtaa aaataaactc tccaaaggcc agcagcgccg
    18721 cgtgaacgcc aatcaccagc gtcgtcttaa aacgtctaag gagaagcccg actacgacga
    18781 caatctgttt ggcgagcctg atgaaggtat cgtcatcagc cgctttggta tgcacgctga
    18841 tgtggaatcc gccgatggcg acgttcaccg ctgcaatatt cgccgtacca tccgttcgct
    18901 ggtaaccggc gaccgcgtag tctggcgtcc gggtaaaccg gcggcggaag gcgtaaatgt
    18961 caaagggatc gtggaagcgg tgcatgagcg tacctcggtg ttgacgcgtc cggatttcta
    19021 cgacggcgta aaacctattg ccgccaacat cgaccagatt gtcattgtct ccgccatttt
    19081 gccggagctg tcgctcaata ttatcgaccg ttacctggtg gcctgcgaaa ccttgcagat
    19141 tgagccgatt attgtgctca acaagataga cctgctggac gacgaaggca tggcgttcgt
    19201 caacgagcag atggatatct accgcaatat cggttatcgc gtattgatgg tttccagcca
    19261 tactcaggat gggctaaaac cgctggaaga ggcgttgacc gggcgcatca gcatttttgc
    19321 cgggcagtct ggcgtcggca aatccagcct gctgaacgcg ttgctggggc tgcaaaaaga
    19381 gatcctgacc aacgatgtct ctgacaactc ggggcttggt cagcacacca cgaccgccgc
    19441 tcggctgtat cacttcccgc acggcggtga tgtgattgat tcccctggtg tgcgtgagtt
    19501 cggcctctgg cacctggagc cggaacaaat cactcagggc tttgtcgaat tccatgacta
    19561 tttaggtctg tgtaaatatc gcgattgcaa acacgatacc gatccgggct gcgctatccg
    19621 ggaagcggtt gaggaaggga aaatcgcgga aacccgtttc gaaaactatc accgtattct
    19681 ggaaagcatg gcgcaggtaa aaacgcgtaa aaacttttct gatacggatg actgacaatt
    19741 aagctaaccg tcgttagaat cgtccccttt ttttcaggat ccggcatgta ggccgaatca
    19801 ggaacgacaa aacaatggcc tggaggctac cttgttaaat tcatttaaac tttcgctaca
    19861 gtacattctg ccgaaactat ggcttactcg cctggcgggt tggggcgcaa gcaagcgggc
    19921 aggatggttg acaaaactgg ttatcgatct gttcgttaaa tactacaagg tcgacatgaa
    19981 agaggcgcaa aagccggaca ccgccagcta ccgcaccttt aacgaattct ttgtccgtcc
    20041 gctgcgtgac gaagtacgcc caatcgatac cgatccgaat gtactggtca tgcctgccga
    20101 tggcgttatc agccagttgg gtaaaatcga agaagataaa atcctgcaag ccaaaggcca
    20161 caactacagc ctcgaagccc tgctggcagg caactatctg atggcggacc tgttccgcaa
    20221 cggtacgttt gtgaccactt acctctcccc gcgtgactac caccgcgtac acatgccgtg
    20281 caacggtatt ctgcgtgaga tgatctacgt gccgggcgat ctcttctccg ttaaccatct
    20341 cacggctcag aacgtgccga atctgtttgc ccgtaacgaa cgcgtgattt gccttttcga
    20401 taccgaattt ggcccaatgg cgcagattct ggtcggagcg acgattgttg gcagcattga
    20461 gacggtctgg gcgggcacca ttacgccgcc gcgcgaaggt atcatcaagc gctggacctg
    20521 gcctgccggg gaaaacgacg gttctgtggc actgctgaaa ggccaggaaa tgggtcgctt
    20581 taaactcggt tccaccgtta tcaacctgtt tgcaccgggt aaagtgaatc tggttgagca
    20641 actggaaagc ctgtctgtca cgaaaatttg ccagccgctg gcagtatcta ccgaaacctt
    20701 tgttacgcca gacgctgaac ctgccccgct tcctgctgaa gagatcgaag cagaacacga
    20761 cgccagccca ttggttgacg acaaaaaaga ccaggtctaa tccatcaaag gaaacgctga
    20821 cgtgcgcctg attatcactt ttctgatggc ctggtgcctc agttgggggg cgtacgccgc
    20881 gacggccccc gatagcaaac aaatcactca ggaactggag caggcaaaag cggcgaaacc
    20941 cgcacagccg gaagtcgtag aggcgcttca gtctgcctta aatgcgcttg aggaacgaaa
    21001 aggttccctt gagcgcatca aacaatatca gcaagttatc gataattatc cgaaactctc
    21061 cgctactctg cgcgcacaat taaacaacat gcgtgacaag ccgcgcagcg tgtcgccggg
    21121 aatgtctacc gacgcgctga atcaggaaat tctccaggtc agcagccagt tgctggataa
    21181 aagccgtcag gcccagcaag agcaggagcg cgcccgcgag attgccgatt cgctgaatca
    21241 actgccgcaa cagcaaaccg acgcccgccg tcagttaaat gagatcgagc gccgcctggg
    21301 aacgcttacc ggcaatactc cgctcaatca ggcacaaaat ttcgcgttgc agtctgactc
    21361 tgcacgtctt aaggcgctcg ttgatgaact ggagctggcg cagctgtctg ccaataaccg
    21421 ccaagaatta gcgcgcttac gctcagagct tgcggaaaaa gagagccagc aactggatgc
    21481 gtatttgcag gccttacgta atcagttgaa tagccaacgt cagcttgagg cggagcgggc
    21541 gctggaaagt accgaattac tggcagaaaa cagtgccgat ttgccgaaag atatcgtcgc
    21601 gcaattcaaa attaaccgcg aactatcggc agcgctgaat caacaggcgc agcggatgga
    21661 tctcgttgcc tcgcaacagc gtcaggctgc cagccagacg ttacaggtcc ggcaggcgtt
    21721 gaatacgctg cgtgaacagt cgcaatggct gggatcgtcc aatctgctcg gtgaagcgtt
    21781 gcgagcgcag gtggcacggc taccagaaat gccaaaacca caacagcttg ataccgaaat
    21841 ggcgcagttg cgtgtgcaac ggttacgtta tgaggatctg cttaataaac agccgctgct
    21901 acggcaaatc catcaggccg acggtcagcc gctgaccgcc gagcaaaacc gtattctgga
    21961 agcacaactg cgcactcagc gtgagttgct gaactcattg ttgcagggtg gcgacacgct
    22021 actattggaa ctgaccaagc tgaaagtctc caacgggcaa ctggaggatg cgctgaaaga
    22081 ggtgaacgaa gcaacgcacc gctatctgtt ctggacctct gacgtgcgcc cgatgaccat
    22141 cgcctggccg ctggaaatcg cccaggatct gcgtcgtctc atttcgctgg acaccttcag
    22201 tcagttgggc aaagccagtg tgatgatgct gaccagcaaa gagacaattt tgccgctgtt
    22261 tggcgcgttg attctggtcg gttgcagtat ttactcgcgc cgctatttca cccgttttct
    22321 tgaacgttcg gcggcgaaag ttggcaaagt gactcaggat cacttctggc tgacgttgcg
    22381 cactcttttc tggtcgattc tcgtcgcgtc accgttaccg gtgctgtgga tgacgctggg
    22441 ttacggcttg cgcgaggcgt ggccttatcc gctggcggtc gcgattggcg atggtgtaac
    22501 ggccaccgtg ccgctgctgt gggtagtgat gatttgcgcc acctttgccc gcccgaacgg
    22561 cttgtttatc gctcattttg gctggccgcg cgaacgcgta tctcgtggga tgcgctacta
    22621 cctgatgagc atcgggctta ttgtgccgct gattatggcg ctgatgatgt tcgataacct
    22681 cgacgaccgt gaattctccg gttcgctggg aaggctttgc tttatcctca tttgcggtgc
    22741 gctggcggtg gtcaccctca gcctgaaaaa ggccgggatt ccgctgtatc tcaacaaaga
    22801 aggcagcggc gacaacatta ccaaccatat gctgtggaac atgatgattg gcgcgccgtt
    22861 ggttgccatt ctggcgtcgg ctgtgggtta tctggcaacg gcacaggcgc tgttagcgag
    22921 gcttgaaacc tcggttgcca tctggttcct gctactggtg gtttatcacg ttatccgccg
    22981 ctggatgctg atccagcgcc gcaggctggc gtttgaccgg gcgaagcatc gccgggcaga
    23041 gatgttagcg caacgtgcgc gtggcgaaga ggaagcgcat catcacagta gcccggaagg
    23101 agcaattgaa gtcgatgaaa gcgaagtcga tctcgatgcc atcagtgcgc aatccttgcg
    23161 gctggtgcgc tcaattttga tgttgatcgc cctgctttct gtcattgtgc tgtggtcaga
    23221 aatccattcc gctttcggct tcctcgaaaa tatttcgctg tgggatgtca cctccacggt
    23281 acagggcgta gaaagtctgg agccaattac cctcggtgcg gtgctgattg ccattctggt
    23341 gtttatcatc accacgcagc tggtgcgcaa cctgcccgcg ctgctggaac tggcgatttt
    23401 gcagcacctg gatttaacgc cgggtacggg ctacgccatc accaccatca ccaaatatct
    23461 gctgatgctg attggcgggc tggtcggctt ctcgatgatt ggtattgagt ggtcgaaatt
    23521 gcagtggctg gtcgccgcgc tcggtgttgg tctcggtttt ggtttgcagg aaattttcgc
    23581 caactttatc tctggtctga ttatcctgtt cgaaaaaccg attcgcattg gcgatacggt
    23641 gacaattcgc gatctcaccg gtagcgtgac gaaaattaac acccgcgcca ccaccatcag
    23701 cgactgggac cgtaaagaga taatcgtgcc gaacaaggcg tttattaccg agcagtttat
    23761 caactggtcg ctctctgact cggtcacgcg cgtagtgttg acgattccgg cccctgctga
    23821 tgccaacagt gaagaagtga cggaaatcct gctcaccgca gcgcgtcgct gctcgctggt
    23881 gatcgacaac ccggcaccgg aagtcttcct ggtggatctg caacaaggga ttcagatttt
    23941 cgagctgcgt atttatgccg ctgagatggg tcaccgtatg ccgctacgcc atgagatcca
    24001 ccagctgatt ctggctggct tccatgccca cggtatcgat atgccattcc cgcccttcca
    24061 gatgcgtctg gaaagcctca acggtaaaca aacggggaga acgctgacgt ctgcgggcaa
    24121 aggtcgtcag gcgggaagtt tgtaatgcat gaagctgcca aatgatgtca acgtggctca
    24181 tttagcagct taacatcatc ttcagttatc accggccctg gagagtatca tccatacgca
    24241 ttacttcctg acaactcatc gccggagaga cgatgagttg caggatttca tatgatcagt
    24301 cgagagctaa ctcaggttta ttcgccatgc gactgcaata gttctggtaa atcgccatcg
    24361 ctaacagcga gaagaagaca gggccgccaa tcatccacaa tgtgctgtcc cagtctccgg
    24421 cttcaaccac aggctgaata atggtgaaga cgttcgcaaa tgtcaccacc agtacgacaa
    24481 ccaccatcgc aatcattgcc gacatacgcg ttttgaaaat cacaaatggt ctgtcgagat
    24541 cctgacgtgc tttaaagaat gggaaagcca gcgcgaggaa caggtaagga agcgtcatag
    24601 acacgttcgc catcagcgtc agcttgttaa agaacgcgga tgcggtaccg ccaccaaacg
    24661 aaaccagcag gatgaagaca gtaaccaacc cgcactgcat ccacatggcg atagacggca
    24721 tccccatcgc attcaggcgc gtcatcggtt ccggccacaa cgctttcggc gtcccctgga
    24781 tgatggcttt cagcggtgaa tagcaaagcg taaagaacgc accggtataa gcgaggaaca
    24841 tcgaaagtcc ggtaatacgc gcaaaccata cgcccagcga caatgacgct tcaggtgaca
    24901 aatgcagtgc gttacccagc gtcaccccga ggctcttcat cagcacatag gtaatattgc
    24961 caaggttaac ggaaccatta cttaatacct gctgccagtt tgtgctgacg ccccataaaa
    25021 atattgccag cgaataaccg attgaaataa caatagcggc aaaaacaata cctttggcaa
    25081 agttcttttc tggattttcc gttttatcga ccagaccacc aaccgcttca attccgccat
    25141 aggcaaaaat agcaaatacc acaaacgata gcattgccag accggactga taacccgggt
    25201 tcggtgatgc aaggaaatta atatcctgcg cgaaatgccc accatttaat aacaaaatag
    25261 taatgcttac taacagcaat actaaattca gacacattac tgcaataccg cccaccgcag
    25321 taatgcgggc aattttatta atcccctttg aagcaacgac ggtgaccaga atcatccatg
    25381 ctactgccag cagaccaacc acctgcgtag gctccagtcc ggcaatacgc cagtgctggg
    25441 tcatgtcgct accatagagg aatgttgaga acggtaccca aactttcgcg gaggtactca
    25501 ccatccagat gatataagag gaaaaccaca taaacgtacc aatgaaggca aaacgtggtc
    25561 cgacactatt attcatccag gaatagatac caccttcttc tttgcgataa gcagctccca
    25621 tttcagccat cat
//
LOCUS       DAKISA010000055        25429 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.055, whole genome shotgun sequence.
ACCESSION   DAKISA010000055 DAKISA010000000
VERSION     DAKISA010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25429
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.055"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            122..445
                     /gene="soxS"
                     /locus_tag="OVW45_002390"
     CDS             122..445
                     /gene="soxS"
                     /locus_tag="OVW45_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCV5526817.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(448..2034)
                     /gene="pdeC"
                     /locus_tag="OVW45_002391"
     CDS             complement(448..2034)
                     /gene="pdeC"
                     /locus_tag="OVW45_002391"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HCV5526818.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            2464..2744
                     /locus_tag="OVW45_002392"
                     /pseudo
     CDS             2464..2744
                     /locus_tag="OVW45_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
     gene            complement(2843..3379)
                     /gene="ssb1"
                     /locus_tag="OVW45_002393"
     CDS             complement(2843..3379)
                     /gene="ssb1"
                     /locus_tag="OVW45_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCV5526819.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            3633..6455
                     /gene="uvrA"
                     /locus_tag="OVW45_002394"
     CDS             3633..6455
                     /gene="uvrA"
                     /locus_tag="OVW45_002394"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCV5526820.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(6490..6846)
                     /locus_tag="OVW45_002395"
     CDS             complement(6490..6846)
                     /locus_tag="OVW45_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCV5526821.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(6850..7266)
                     /locus_tag="OVW45_002396"
     CDS             complement(6850..7266)
                     /locus_tag="OVW45_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCV5526822.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(7377..8090)
                     /gene="aphA"
                     /locus_tag="OVW45_002397"
     CDS             complement(7377..8090)
                     /gene="aphA"
                     /locus_tag="OVW45_002397"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCV5526823.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            8492..8714
                     /locus_tag="OVW45_002398"
                     /pseudo
     CDS             8492..8714
                     /locus_tag="OVW45_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9218..10411)
                     /gene="tyrB"
                     /locus_tag="OVW45_002399"
     CDS             complement(9218..10411)
                     /gene="tyrB"
                     /locus_tag="OVW45_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCV5526824.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(10664..11743)
                     /gene="alr"
                     /locus_tag="OVW45_002400"
     CDS             complement(10664..11743)
                     /gene="alr"
                     /locus_tag="OVW45_002400"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCV5526825.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSIGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(11796..13211)
                     /gene="dnaB"
                     /locus_tag="OVW45_002401"
     CDS             complement(11796..13211)
                     /gene="dnaB"
                     /locus_tag="OVW45_002401"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCV5526826.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            13294..14277
                     /locus_tag="OVW45_002402"
     CDS             13294..14277
                     /locus_tag="OVW45_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCV5526827.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(14443..14685)
                     /gene="pspG"
                     /locus_tag="OVW45_002403"
     CDS             complement(14443..14685)
                     /gene="pspG"
                     /locus_tag="OVW45_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCV5526828.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(14819..15856)
                     /gene="dusA"
                     /locus_tag="OVW45_002404"
     CDS             complement(14819..15856)
                     /gene="dusA"
                     /locus_tag="OVW45_002404"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCV5526829.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(16219..17204)
                     /locus_tag="OVW45_002405"
                     /pseudo
     CDS             complement(16219..17204)
                     /locus_tag="OVW45_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
     gene            17522..18037
                     /gene="zur"
                     /locus_tag="OVW45_002406"
     CDS             17522..18037
                     /gene="zur"
                     /locus_tag="OVW45_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCV5526830.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(18079..18288)
                     /locus_tag="OVW45_002407"
     CDS             complement(18079..18288)
                     /locus_tag="OVW45_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCV5526831.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(18404..19729)
                     /gene="dinF"
                     /locus_tag="OVW45_002408"
     CDS             complement(18404..19729)
                     /gene="dinF"
                     /locus_tag="OVW45_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCV5526832.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMSKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(19802..20410)
                     /gene="lexA"
                     /locus_tag="OVW45_002409"
     CDS             complement(19802..20410)
                     /gene="lexA"
                     /locus_tag="OVW45_002409"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCV5526833.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(20520..20888)
                     /gene="dgkA"
                     /locus_tag="OVW45_002410"
     CDS             complement(20520..20888)
                     /gene="dgkA"
                     /locus_tag="OVW45_002410"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCV5526834.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            21059..23482
                     /gene="plsB"
                     /locus_tag="OVW45_002411"
     CDS             21059..23482
                     /gene="plsB"
                     /locus_tag="OVW45_002411"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCV5526835.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRPLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(23637..24509)
                     /gene="ubiA"
                     /locus_tag="OVW45_002412"
     CDS             complement(23637..24509)
                     /gene="ubiA"
                     /locus_tag="OVW45_002412"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCV5526836.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(24522..25019)
                     /gene="ubiC"
                     /locus_tag="OVW45_002413"
     CDS             complement(24522..25019)
                     /gene="ubiC"
                     /locus_tag="OVW45_002413"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCV5526837.1"
                     /translation="MSHPALTQLRALRYFKEIPALDSQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(25242..>25429)
                     /locus_tag="OVW45_002414"
     CDS             complement(25242..>25429)
                     /locus_tag="OVW45_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5526838.1"
                     /translation="MLNPTKDTNWNSTCIYKSRHKMLPINLTQETLFSSKSHGKYALF
                     PIFTASWRATRIKNIGI"
BASE COUNT         6583 a         6541 c         6086 g         6219 t
ORIGIN      
        1 tagcagcgct ttaatgcggg gtaatttctt ttccataaat cgctttacct caagttaact
       61 tgaggaatta tactccccaa cagatgaatt aacgaactga acactgaaaa gaggcagatt
      121 tatgtcccat cagaaaatta ttcaggatct tatcgcatgg attgacgagc atattgacca
      181 gccgcttaac attgatgtag tcgcaaaaaa atcaggctat tcaaagtggt acttgcaacg
      241 aatgttccgc acggtgacgc atcagacgct tggcgattac attcgccaac gtcgcctgtt
      301 actggccgcc gttgagttgc gcaccaccga gcgtccgatt tttgatatcg caatggacct
      361 gggttatgtc tcgcagcaga ccttctcccg cgttttccgt cggcagtttg atcgcactcc
      421 cagcgattat cgccaccgcc tgtagtttta ttgcccgcgc gttaactccc gcgcgggtaa
      481 ttgctccatc cattgcataa acacctgcgg cggcatcgcc ttcgcaaaaa accatccctg
      541 gcaatagcgc acgccgcgtt tgcgcagcca gttaacctgc tcctcagttt cgacgccttc
      601 agcgatcgtt tttaacccca ggctgtgcgc cagctcgatg atgtgttccg caatcaaatg
      661 actggttttg tgggtggtca gcgtttcaac aaacgattta tcgattttca aaatatcgac
      721 attcaatgat ttaaggttat gcaagttaga gtagccaata ccaaaatcat caattgccac
      781 ttcgtaacca gcctggcgga aagccagaat aatcggcgtc attttttcga catcaagaaa
      841 tgcatgctca gtcacttcaa atttaatttg ctgcggacgc accgcgtatt gctctgtttt
      901 ctgattgatt cgcgctatca accgtgacgt atgaaaatcg gaggccgaca ggttaataga
      961 aacatagcga tcggcatgtg ttgccaagta cgcgcccaga tcgcggaaga cattatcaat
     1021 aacataatca gttatctgtt ctatcatccc ctccttttct gccagcggaa taaactctgc
     1081 cggattcatt atttgcccct gctcaccaag ccaacgtaac aacgcttcag cgccgataca
     1141 tttttctgtt ttgatatcga ttattggctg gtaataaaga caaagttgat gtttttcgag
     1201 ggcgcgttgc aatttacgtt tgggagataa atagttttgt cgaatacgta gccagagtaa
     1261 tagcagaaca agactcccca aaataccggc gggcaacgca aaaataagat gattataaaa
     1321 atgggttata agacgttgat atgaagtcgc aacaatggct gcaattgggc gttttgtcga
     1381 ataaactgtc gcatataaat agccatttct ttgtacggtt aaatccttca aatgaatcag
     1441 cggagaaaac gttgctgccg aggcctcttt gcttaacgag aaaaaggttt tcgtcaccgt
     1501 atcatacaca ccccattgca atgtcgggtc atcagacatc acttcactcc agaagagagg
     1561 gttgataacc gccacataat ttccccgctg catataggtc attttatagc cagagaaaaa
     1621 aggcgtatcg cggtaataat agatagaaac gttaggttca cgcttataat cggccggtgc
     1681 aatcgtatag ccgtttacag gcgctatcag cgatgagcat aaaaaatggt tatcacgggc
     1741 atagatcaat tcattaatat aaagatagcc acgaataata ttcaacattc gcttttgatg
     1801 ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta aatctgcctg
     1861 gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga gctgattatt
     1921 cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg gaaagagaac
     1981 taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac tcattgctat
     2041 caatttcctg cctgtttata cccggccatt gcaaggtgaa aaccctgggt accgacgtct
     2101 ttagataaat cttacggtat aaatagcact tttgcctgat ttgaaattga tacctttcta
     2161 atcgtcacct ggatgaaaat aattcattga tattcaaaat taatttccta caggaatgca
     2221 gaggcggcgg gaagatatgc ccttaatgcc actgaaaaac cacataatct gtacagcaat
     2281 aaaagtcacg gcctaatatg gctaacaaac aatcaattgt gatatagctc acaaaattaa
     2341 tgaaacaaac agagtgtttc atttttgtgt tctgcaagcc ccgcgatagc cagagtgtac
     2401 cacgcctccc gtgaacaacg ccgcgctgtc cagggcatcg gctcttttca gaggaattgt
     2461 ttgatggcta ccctcaccac tggcgtggtt cttcttcgct ggcagcttct tagtgccgta
     2521 atgatgtttc tggccagcac atcaacatcc gttttcgtcg gtctgattat gtcgggcttg
     2581 cggtgatcag cagcggtctg ggcgttgttt ccgcctgttg gttcgcaatg gggttgcttg
     2641 gcatcacaat ggcggacatc accgctatct ggcacaacat cgagtcggtg atgatagaag
     2701 agatgaatca gacaccacca caatggccaa tgattttgac ctgatacgta gaagcctcca
     2761 taacggaggc ttctttttta tggctggcag atgctttaat catccacctt aaaacaatat
     2821 aacctattgt tttaatgaca aatcagaacg gaatgtcatc atcaaagtcc atcggcggct
     2881 cgttagacgg cgctgccgga gcggactgct gcgggcgaga ctgcgcgccg ccgctgaact
     2941 gattgccacc ctgcggctgc tgaggctgac cccaaccgcc ctgcggctga ccaccaccga
     3001 tattaccacc tgccggagcg ccaccaccct gacgaccacc cagcatctgc atggtgccgc
     3061 caacgttcac cacgacttct gtggtgtagc gatcctgacc ggattgatcg gtccatttac
     3121 gggtacgcag ctgaccttcg atataaacct gagaaccttt acgcagatat tcgctggcca
     3181 cttctgccag tttgccgaac agcacaacgc ggtgccattc agtctgttct ttcatctcgc
     3241 cggtcgcttt atcacgccag gattcggaag tagccagcgt aatgttggca actgcgccac
     3301 catttggcat gtagcgtact tccgggtcct gacccagatt accaacgaga ataaccttgt
     3361 ttacgcctct gctggccatg ttcgtgtctc ctgaaaaaaa tcgttctgaa taagtgtaaa
     3421 cgcgcgattg taccattacc aatagcgctt ttactatgtt gtgacctcgg ttccgggaaa
     3481 caaacctggc cagacattgt tacacaacac tccggataat gcattccaat actgtatatt
     3541 cattcaggtc aatttgtgtc ataattaacc gtttgtgatc gccggtagca ccatgccacc
     3601 gggcaaaaaa gcgtttaatc cgggaaaggt gaatggataa gatcgaagtt cggggcgccc
     3661 gcacccataa tctcaaaaac atcaacctcg ttatcccccg cgacaagctc attgtcgtga
     3721 ccgggctttc gggttctggc aaatcctcgc tcgctttcga caccttatat gccgaagggc
     3781 agcgccgtta cgttgaatcc ctttccgcct acgcgcggca gtttctgtca ctgatggaaa
     3841 agccggacgt cgatcatatt gaggggcttt ctcctgccat ctcaattgag cagaaatcga
     3901 cgtctcataa cccgcgttct acggtgggga caatcaccga aatccacgac tatttgcgtt
     3961 tgttgttcgc ccgcgtcggc gaaccgcgct gtccggacca cgacgtcccg ctggcggcgc
     4021 aaaccgtcag ccagatggtg gataacgtgc tgtcgcagcc ggaaggcaag cgtctgatgc
     4081 tgctcgcgcc aatcattaaa gagcgcaaag gcgaacacac caaaacgctg gagaacctgg
     4141 caagccaggg ttacatccgt gctcgtattg atggcgaagt ctgcgatctt tccgatccgc
     4201 cgaaactgga actgcaaaag aaacacacta ttgaagtggt ggttgatcgc ttcaaggtgc
     4261 gtgacgatct tacccaacgt cttgcggagt cgtttgaaac cgcgctggag ctttccggtg
     4321 gtacagcggt agtggcggat atggacgacc cgaaagcgga agagctgctg ttctccgcca
     4381 acttcgcctg cccaatttgc ggctacagta tgcgtgaact ggagccgcga ctgttttcgt
     4441 ttaacaaccc ggcaggtgcc tgcccgacct gtgacggcct tggcgtacag caatatttcg
     4501 atcctgaccg cgtgatccaa aaccccgagc tgtcactggc tggcggtgcg atccgtggct
     4561 gggatcgccg caacttctat tatttccaga tgctgaaatc gctggcagat cactataagt
     4621 tcgacgtcga agcgccgtgg ggcagcctga gcgcgaacgt gcataaagtg gtgttgtacg
     4681 gttctggcaa agaaaacatt gaattcaaat acatgaacga tcgtggcgat acctccattc
     4741 gtcgtcatcc gttcgaaggc gtgctgcaca atatggagcg ccgttataaa gagacggaat
     4801 ccagtgcggt acgtgaagaa ttagccaagt ttatcagcaa tcgcccatgc gccagctgcg
     4861 aaggaacgcg tctgcgtcgg gaagcgcgcc acgtgtatgt cgagaatacg ccgctgcctg
     4921 ctatctccga catgagcatc ggtcatgcga tggaattctt caacaatctc aaactcgcag
     4981 gtcagcgggc gaagattgca gaaaaaatcc ttaaagagat cggcgatcgt ctgaaattcc
     5041 tcgttaacgt cggcctgaat tacctgacac tttcccgctc ggcagaaaca ctttccggcg
     5101 gtgaagccca gcgtatccgt ctggcgagcc agattggtgc gggcctggtt ggcgttatgt
     5161 atgtactgga cgagccgtct atcggcctgc accagcgcga taacgagcgc ctgttgggta
     5221 cgcttatcca tctgcgcgat ctcggtaata ccgtgattgt ggtggagcat gacgaagacg
     5281 caattcgcgc cgctgaccat gtgatcgata tcggtcctgg tgcgggtgtt cacggcggtg
     5341 aagtggtcgc agaaggtccg ctggaagcga ttatggcggt gcctgaatcg ttgaccgggc
     5401 agtacatgag cggtaaacgc aagattgaag tgccgaagaa acgcgttccg gcgaatccag
     5461 aaaaagtact gaagctgaca ggtgcacgcg gcaacaacct gaaggacgtg acgctcacgc
     5521 tgccagtcgg tctgtttacc tgcatcacag gggtttcagg ttccggtaaa tcgacgctga
     5581 ttaacgacac actgttcccg attgcccaac gccagttgaa tggtgcgacc atcgccgaac
     5641 cggcaccgta tcgcgatatt caggggctgg agcatttcga taaagtgatc gatatcgacc
     5701 aaagcccaat tggtcgtact ccacgttcta acccggcgac ctataccggc gtgtttacac
     5761 ctgtgcgcga actgtttgcg ggcgtaccgg aatcccgtgc gcgtggttat acgccaggac
     5821 gtttcagctt taacgtccgt ggcgggcgct gcgaagcctg tcagggcgac ggtgtgatca
     5881 aagtggagat gcacttcctg ccggatatct acgtgccgtg cgatcagtgc aaaggtaaac
     5941 gctataaccg tgaaacgctg gaaattaagt acaaaggcaa aaccatccac gaagtgctgg
     6001 atatgaccat cgaagaggcg cgtgagttct ttgatgcggt gccagctctg gcgcgtaagc
     6061 tgcaaacgtt gatggacgtt ggtctgacgt acattcgcct ggggcagtcc gcaaccacac
     6121 tttctggtgg tgaagcccag cgcgtgaagc tggcgcgtga gctgtcaaaa cgcggcaccg
     6181 ggcagacgct gtatattctc gacgagccga ccaccggtct gcacttcgcc gatattcagc
     6241 aactcctcga cgtgctgcat aaactgcgcg atcagggcaa taccattgtg gtgattgagc
     6301 acaatctcga cgtgatcaaa accgctgact ggattgtcga cctgggacca gaaggcggca
     6361 gtggcggcgg cgaaatcctc gtctccggta cgccagaaac cgtcgcggag tgcgaagctt
     6421 cgcatacggc acgcttcctc aagccgatgc tgtaatcgtt aaggccgctt tctgagcggc
     6481 cttttccttt cagagttgta ccagcaattt acgcttttct tccggtagta aattcactgc
     6541 ctgctgataa gacgcatcca ccagataata gatttgcgaa tccggcagcg aaccgtcgag
     6601 atacacggtg ctccagtgcg ctttattcag atggcggcta ggacgcacat cgctgtgctg
     6661 ctgacgtagc agctccgcca gctccgggct ggttttcagc gaaacagctg ggcgattttc
     6721 tacttctttc accatcgcaa acagtacatc ttccactttg atctgcgtcg ctttccagtc
     6781 gttatgcacg ctctgttctg cgcctggttt tgccatgcaa tattgtagca actccgaaat
     6841 ggtcattttt tactcccctt gtagtgtcgc gatgatgcga cgcgatccgc cgtggatgcg
     6901 atgttccccc agccagatgc cttgccaggt gccggtctga atacgccctt tatgtaacgg
     6961 caatacaagc gatgttccca gcattgagga tttgatatga gaaggcatat cgtctgctcc
     7021 ctcatagtca tgctcataat ttccgttgtc gggaacggtg cggaggaaaa aacgctccat
     7081 gtcgtggcgt acggtgggat cgcagttctc attaagtgtc agagaggcgg aggtatgttg
     7141 cagcaacaga tgcagtaagc cgatgttaac gcgcggcata tcagccagct gattcagaat
     7201 ttcatccgtt accagatgaa acccacgaga tttggcgcta agcgtgagcg tcttttgata
     7261 ccacatgtgc tgctccttga taaaactctc ttaatcagtt tgcagcaaga cagcgaaagg
     7321 ataaaggtgt gattaaaaaa acagcattcg ggagagcatc acgctctccc gctctgtcag
     7381 tattctgaat tgacgatcac ctcttcacca aacgcacccg cttgtggcaa gggtttgtag
     7441 gtagagttgg aggcgcgcag aatgcggata ccacgagcgc cgacatcgcg tgcggcggta
     7501 atatcgttat cggaatcgcc ataaaaaatt cggatatttt tatcctgcag ccattgcgat
     7561 tttgtatttt gccctggttt atcgcccgca aagattaccg gattcatgtt ggtggcagga
     7621 atatgaaaat tatccgccag cgtttttgaa accgtttctg ttttcgtcgg gctacgacca
     7681 gtcacaaaga agatcgcgtc accgcggcgt acatgcatat caatcagctg gcgagcgacc
     7741 tcttttggaa tgctgaattc atcccagcca ttattcattt tttcccagaa cacaggattt
     7801 ttcagataat cttcgctttc tggcgagaag gtttttttgc cgcgccagaa ccccggactg
     7861 gaaaaaagta ccgtgtcatc gatatcaaac cccaccgcca ttggcggacg ccctgcgagg
     7921 ctattttcaa tttgtgcgac cgaaacccaa tgaattggtg cctgttcagc aagcctggca
     7981 acgttagtcc cagggttaag cggtgaagga gatgaggcca gggcaacagc ggaactgttt
     8041 agagcgaaca ataagcaaac ggcactgatt gcctgtgtga tcttgcgcat atttttccct
     8101 aaatagtcag tatgttgaaa ctttttattg tgagatttgt tgcaaaaacc atctgaccat
     8161 aacgccagct gccggggaaa ggaaggatta tctgcggttt ttgtgatgaa aagcaggatg
     8221 attatattta acccagtaaa tacgttcctg tctttttttg tataaaaaat gtttattact
     8281 cgatgtaagg atacattaat tttacgttta ttatgatgaa tcctctgcaa atgagcgaaa
     8341 tttgaaagaa catttatctg ctgtacgatt taatgaactt aatgatacag cattacaaaa
     8401 acaatcgaag tttataaaga tgattcctga ttgaccagaa ccctatccta caatggtgac
     8461 taattttatt actcccgatg gagatgatga tatgaatata tcatatgtga atagcaacaa
     8521 aacgacatct ttacctgttg agctagatgc actaaataat aaagacatct cttatgccaa
     8581 agatttttct tatgctaaag atttcttttt atacatagaa acacagttaa aaattgcaaa
     8641 agatttttgt agacctggag aagaagtatc aagttctatt gcaagtaaag tttttcacgc
     8701 atttatcgat ttagttaaca aaatcagggg taagaaagac tttatgtata tttgcacgct
     8761 ttgctgtttt gccgaggagg ttaaaggtga ttattctcat tacaggacct ttttatttga
     8821 tattggtaat caatataagg ttaaacttac acaaagcgga aaaaaagagt tctctttaac
     8881 attagaattt aacgatacta taattgaatc tcagaaagtc acaggcaata aggcaaagta
     8941 tattcttgag gatatagaaa tattctatcg taacaaaccc gatacttatt attaaattta
     9001 ataatggtta gatgtgcaga aattattact ccacacgaaa aaatagggaa aggaattcca
     9061 gataattggg ataaacttaa caataaaaga actgacaacg ttttattatc tctcacgtag
     9121 aacaacggca tcaaaaaaat catctacaaa gaaatagtga aagcgacgac agtttaattt
     9181 cactgcaggc tgggtaactc cagcctgctt tcctgcatta catcaccgca gcaaacgcct
     9241 ttgccacacg ttgtacattt gccgtattta acccggcgac acacatgcga ccgctggcga
     9301 tgagatagac accaaattct tcacgtagtc ggtcaacctg agcggcactt aaaccggtat
     9361 aactgaacat gccgcgctga ttaagcagat aatcgaaatt gcgttctggc atctctgtgc
     9421 ttaatacctt caccaattcc tgacgcattg ccagaatgcg agtacgcatc tcttctactt
     9481 ccgccagcca gctggctttc aatgcctcgt cattcagcac tgcagccacc acctgcgcac
     9541 caaaattcgg cgggctggag tagttgcggc gaactgttgc tttcaattgc cccagtacgc
     9601 ggccagcggc ttcggcatct tcacacagaa cagaaagtcc gccgacgcgc tcgccgtaaa
     9661 gggagaaaat tttcgagaac gaattgctca ccagagcggg taatccagcg ctggcaatgg
     9721 cgcgaatagc gtaggcatcc tcttccatac cggcactaaa tccttgatag gcaatatcga
     9781 ggaatgggat aagctcgcgg gctttgagaa tttcaatcac cgcatcccac tgatcattag
     9841 tgagatcggc acccgttggg ttgtggcaac atggatgcag caacacaata ctgcgggcag
     9901 gtaatgtttt cagcgtcgcc aacaggtcat taaagcgcac gctgttagtc gcttcgtcat
     9961 accaggggta agtactcact tcgaatccag ccccggcgaa tattgctacg tggttttccc
    10021 aggtaggatc gctgacccag acgcctgatt ccgggaagta gcgtttcagg aaatccgcgc
    10081 ccactttcaa tgcccctgag ccgccaaggg tttgaatggt tgctacgcgc tgttgttgca
    10141 gtaccggatg gtccgcacca aacagcagcg gcgcaatggc atggcgatag ctgttaagcc
    10201 cttccatcgg taaataaagc gaagcgccat ggggctgcgc attcaggcgc gcttccgcct
    10261 ccgccacggc tttcagttgt ggaataattc cgtcttcgtt gtagtacaga ccgatactta
    10321 aattcacttt gtcgctgcga gggtcttctt taaaacgctc cataagcgta agaatcgggt
    10381 cgccagcgta ggcgtcaact ttttgaaaca cgcgatggtt ctccaggttt acgggcaggt
    10441 ggttaaaaca caataaaccg gaagaaggcg aagatcgagt ggatgttcag gagcgaacgg
    10501 caattagcaa cagagtgaga ctcatgacaa acgaacatcc gccagaggca cgacctttat
    10561 aagagcatga aagaacatca acttattgaa tttttaggat tttattggcc ggataaggca
    10621 ttcacgccgc atccggcaca gacaatcaaa tattacagaa cgattaatcc acgtatttca
    10681 tcgcgaccct tgaagtcagg cgcgtaataa gttcgtaagc gctcactttt gtcatttcag
    10741 cgatacgttc tacgggcaaa ccttcgcccc ataaaatgac cggatccccg gctttgtcct
    10801 gcgcctgtgg acctaagtct acgcagatca tatccatcgc cacgcgtccg acaatcggta
    10861 cttcgcgacc gttcaccagc actggcgtac cggatggagc ggcgcgcgga taaccatcgc
    10921 catagcccat cgcgactacg ccaagacggg tatcacgttc gcttacccag gttccaccat
    10981 aaccaacagg ctctccggct ttatgctcac gcacggcaat caggctggag gttagtgaca
    11041 tcactggctg acagccaaaa tcggcaccga tggagcgatc ttccagcggc gagacgccat
    11101 aaagaatgat gcccgggcgc acccagtcaa aatgcgattg tggccacagc agaatgccac
    11161 ccgacgcggc aatggaacgt tgaccaggtt tgccttcgca aaaggtatta aagatagcga
    11221 gttgtttctc ggttgcgcca cattttggtt catccgcgcg cgcaaaatgg ctgacgatat
    11281 tcaccggctg acgaacgttt ttgcactgtg tcaggcgatg ataaaacgcc tcagcctgtt
    11341 ccggccttac gcccagacgg tgcataccgg tatcgagttt catccagacg gtaaccggct
    11401 cgtccaggct agcctcttcc agcgcagcca gctgttcttc gttatgcacg gcggtatgaa
    11461 aatgttgcgc agaaatcgtc ggcagatctc tggcatcaaa aaagccttcg agtaacagta
    11521 caggtttggt gattcccccc gcacgcagtc gcagagcttc ttcgagacgg gctacgccaa
    11581 aggcgtcagc atcggggagc gttcgcgcgg tctcaagaag accgtgacca taagcgttcg
    11641 ctttcaccac cgcaaccatt ttactggcag gggccagttc acgaagacgt tgcaggttgt
    11701 gtcgcagagc gcggcggtta atcacaacag ttgccgcttg catttgtgtt ccttgataag
    11761 tgtttgcttt aattacctaa ttcataaaat aattattatt cgtcgtcgta ctgcggcccc
    11821 gcatagttgt cgaagcgcga ccattgaccg ttaaaggtca ggcgtaccgt cccgattggg
    11881 ccgttacgtt gtttaccgat aataatttcc gcgatgcctt ttaaatcact gttttcgtga
    11941 tacacctcat cacgatagat aaacataatc aagtccgcat cctgctcgat agagccagat
    12001 tcacgcaggt cggagttgac cgggcgtttg tcggcacgtt gttccagaga acggttcaac
    12061 tgggacagcg ccaccaccgg cacgttcagt tctttcgcca gtgctttcag cgagcgagag
    12121 atttctgcaa tttccagcgt acggttatcg gaaagcgccg gtacgcgcat cagttgcagg
    12181 tagtcgatca tgataagccc gatgccgccg tgttcacggg caatacggcg tgcgcgggaa
    12241 cgcacttccg ttggcgtcag gccggaggag tcatcgatat agatattgcg tttttcgagc
    12301 aaaataccca tggtgccgga aatgcgcgcc cagtcttcat catcgagctg cccggtacgg
    12361 attttagtct ggtcaacgcg cgacagcgac gccagagaac gcatcataat ctgttctgaa
    12421 ggcatctcca gcgagaagat aagtactggt ttatcctgca acatcgccgc gttttcgacg
    12481 aggttcatcg caaatgttgt tttacccatc gacggacgcg cggcgacgat gatcaaatcc
    12541 gacggctgca agccagcggt ttttttgttg agatcgtcat aaccggtgtt tactccggta
    12601 acgccatcgt gtggctgctg aaacaactgc tcaatacgcg ccacggttgc gtcgagcaca
    12661 tcggcgatgt tcttcggccc ttcgtctttg tttgcacgac tttcggcaat tttaaagacg
    12721 cgggattcag caaggtccag cagatcttcg ctggtacgcc cctgcggatc aaaaccggct
    12781 tcagcaatct cattcgcaac cgagatcatc tcacggacaa cggcacgttc acgcacgatg
    12841 tcagcataag cactgatgtt cgccgcactt ggcgtatttt ttgacagctc tgccagataa
    12901 gcaaaaccac cgacgctatc gagttgcccc tggcgttcca gcgattccgc aagggtaatc
    12961 agatcgatag gactaccgct ttcctgcaaa cgcgccattt cagtaaagat atggcggtgt
    13021 gggcgggtgt aaaaatcgtc tgccaccaca cgctcggcta catcgtccca gcgttcgtta
    13081 tccagcatta aaccgcccaa caccgactgc tccgcttcga tcgagtgcgg aggcactttc
    13141 agcccggcaa cttgtggatc gcgttcgcgg ggttcagcct gctgtttgtt gaagggttta
    13201 tttcctgcca tagtgaatgg agttaccgag ataaagaatg ggtcgaaact ttaccatatg
    13261 aagcagaccc tgacgatacg ttctggagga cacatggcaa cacgaattga atttcacaag
    13321 cacggtggcc cggaagtact tcaagccgta gagttcactc ctgccgatcc ggcagagaat
    13381 gaaatccagg tcgaaaataa agccatcggc atcaatttta tcgacacgta tatccgcagc
    13441 ggcctttacc cgccgccatc actacccagc ggattaggta ccgaagcggc aggcatcgtg
    13501 agtaaagtcg gcagtggtgt aaagcatatt aaggcaggcg atcgtgtagt ctatgcgcag
    13561 tcggcgttag gcgcttacag ctctgtgcat aacattaatg cggataaagc ggcgattctg
    13621 cctgcggcaa tttcttttga gcaagctgcg gcatccttcc tgaaaggctt aacggtttat
    13681 tatctgctgc gcaaaaccta tgaaattaaa cccgatgaac agttcctgtt ccacgcagcg
    13741 gctggcggcg ttggcttaat tgcctgccag tgggcaaaag ccctgggcgc gaaacttatc
    13801 ggcaccgtag gaaccgcgca aaaagcgcag agcgcgctaa aagcgggcgc gtggcaggtt
    13861 attaactatc gtgaagagga tctggtcgag cggttaaaag agatcaccgg cggtaagaaa
    13921 gtgcgcgtgg tgtacgattc cgtgggcaga gacacctggg aacggtcgct ggattgcctg
    13981 caacgccgcg gcttaatggt cagttttggc aactcatcag gtgcggttac cggtgtgaac
    14041 ttaggcattc tcaatcaaaa aggctcgttg tatgtgacac gcccttccct gcaaggctat
    14101 atcaccacgc gggaggaatt aaccgaggcc agtaatgaac tgttctcttt gattgccagc
    14161 ggtgtgatta aggtcgatgt cgccgagcag cagaaatatc cgctgaagga tgcgcagcgt
    14221 gcgcatgaga ttctggaaag ccgggcgacg caaggttcca gcctgttgat tccataaaag
    14281 aaatagggct tccacctggg aagccctttc tttttatagt tcggctgtat gtagggtaca
    14341 gcacgatgaa tctgttagag gcgcaatagt gacagatttg attatcaatt cctattttgt
    14401 tctaaggata aaaccttagg ttgtgatcat ccgcacaatc ccttagtaac gccagcggtc
    14461 ataacgctga tatttcggca cttttggtgc tttaatcgcc ttaataaccc acaccaccgc
    14521 aatcgccagt agtaaccacg gcagcaactt aatcatcaat gccagcatac cgccgaggaa
    14581 cataatggcc gtcgccacaa ccagcgcggc gataatgccc agcaacgaaa cgccggtgac
    14641 catcagcatg acaaaaaagc caatcacaaa aagtagttcc agcatgatgc tctcccaaat
    14701 atgaaatctc ttgctggcat tacaagaatc atgccaaaaa taatctattg atttaacagc
    14761 aaaacgcccc gcgacggtgc gcagggcgtg gtgaatttga ctactttttg gtgaaaagtt
    14821 aacgcttatc cgccaccagt ttgagcgcgt gttccagcac attaatgtct gcacccgctt
    14881 tatgggcatt ttcacttaaa taacgccgcc actgccgcgc gccaggaata ccctggaaca
    14941 agcccaacat atgccgggta atatggccga gatacgtccc ctggctgagt tcacgctcaa
    15001 tgtacggata catggcgcgc actaccgcca ccggatcggc atcggtatcc gaggaaccaa
    15061 agatctcccg gtctaccgcc gccagaatac ccggattctg atacgcctcg cgcccgacca
    15121 tcacgccatc catatgttgc aggtgtgctt tggcctcttc cagcgacttg ataccaccgt
    15181 taatcgacat tgtcagatgc ggaaagtcac gcttcagttg atacacacgc ggataatcga
    15241 gcggcgggat ttcacggttt tctttcgggc ttaaccccga aagccaggct ttacgtgcgt
    15301 ggatgataaa catctcacac tcgcctttgc cggaaacggt gttgatgaaa tcgcagagaa
    15361 attcatagct gtcctggtca tcgatgccaa tacgcgtttt caccgtcact ggaatcgaca
    15421 ccacatcgcg catcgctttc acgcagtcgg caaccagctg cgcattaccc atcagacacg
    15481 caccaaacat gccgttctgc acccggtcag acgggcagcc gacattcagg ttgatctcat
    15541 catatccacg cgcttctgcc agcttcgcac actgtgccag cgccgccgga tcgctaccgc
    15601 cgagttgcaa cgctaccgga tgttcttctt cactgtacgc caggtaatca cctttaccgt
    15661 gaataatcgc ccctgtggtc accatttcgg tatacagcaa cgtattgcgg gaaagcagac
    15721 gcaagaaata gcggcaatgt ctgtccgtcc agtcgagcat tggtgcaacg ctaaaccgac
    15781 cactccagtg aacgtcagtt ttttcaggca ttgcgctggt ttggttgatt ttttgcattt
    15841 cagaattacc gtgcattttc aaatgtagag attattttat cgatatatca tggggttatg
    15901 ttattcagca tcactattca ggaggctcaa tagcggggta ctataccata acaacaggaa
    15961 gcgcctgtct cattgcaaaa gaaaattgag atcctctcaa ggcatgaagc tctcacgaag
    16021 tgatggaaat aatcttatta gccgttaact ttgttaaggc caatgataaa caatccaggt
    16081 tcgacgataa ataaaaaatc acacattaaa ctctggtgat atatctccct gctaatagca
    16141 ttgatagaga aaaaagaacc caataaatat tgggtccttt tatataatgc cttccatact
    16201 atcgaagaac ttcacatatt atttctccga tttaaccccg aacaaatcat aaattaattt
    16261 agaagtgtct gtaagtattt taatctcttc ctttgaggtt gggtcaaacg acttcgcgaa
    16321 gctaattaat tgtggtgcag catctcgcat tttgcttaaa atatcaggat cgagcgttcc
    16381 ttcattcacc agatgtgaca tcttatccag atagccatca aaattcattt ctcccccatc
    16441 agccagacgc ttcatctctc ccaggtactt cttcatatca cgttgagata atttttcaaa
    16501 ctgagctttc aggtagctct cattattttc attaatatac actgtttctg ataaatctgc
    16561 gtaggcacta gaatatgaaa gtgaaattaa cagcgaagca aataatttta agctgttgtc
    16621 atcatgttcc tggcaggcat taacaaacgt taaaaaacca gaacctattg cttgaaaatg
    16681 tatattcgct aaaggttcat tatccttaga ttcctcataa aatagctgaa ccgtggaggg
    16741 aaggccattc ttagtttcta catcaaaagt acattgttca atgggagtcg catctttatt
    16801 atccctagga ttacatatac tattaatcgc gaaatgaaaa atagcccggt caacaactga
    16861 tgcaataaag tcacgatcgt ttaaatcctc atcagtgcaa tcgtcaatat acttattgac
    16921 ccacattttg agatcattta tttgctcaat taaaaacaca tttttttacg tgcatgccat
    16981 aaattatgcg tgattcgttg aataaacaaa tctatttttg caataattat atcccgatga
    17041 cgcagcgctg cataatcaat agatttacca tatatattat ttaatttacc tttgattgaa
    17101 cctctattat ctaatttatc tttgattgca tcttcattat cgaattgatt tgaattgagt
    17161 gttttattaa agtatccagt tgcaccgggt attatcttca acatgattca tctacctttt
    17221 ttgtcgtacc gtgtcctcct tataattctt ttgccatctg gtcactatca aaaatcgctc
    17281 ataatttctg aactacaggt atgcgtttaa acccagttaa ataaatagcg agttaacttg
    17341 aagcaattta tttaaaaatg aattattaat tattatcgtg ttgataatgg tacgacaagg
    17401 caacataaca ccttacctac aaaacagaaa atcaacatta cggcaacaat aagggttctc
    17461 ggtgtttttc ctggaacatg gtaaagtaag gacattctta acccccactt tgaggtgccc
    17521 gatggaaaag accacaacgc aggagttatt agcgcaggct gaaaaaatct gcgcgcagcg
    17581 taatgtgcgc ctgaccccac agcgcctgga agtgttgcgc ctgatgagtc tgcaggatgg
    17641 cgctatcagc gcttatgatc tgcttgattt actgcgcgaa gctgaaccgc aagccaagcc
    17701 gccaacggtt tatcgcgcgc tggattttct gcttgagcaa ggttttgtgc ataaggtgga
    17761 atccaccaac agttatgtgc tctgtcatct gttcgaccag cccacgcata cgtcagccat
    17821 gtttatatgc gatcgctgcg gcgcggtgaa agaagagtgt gcagaaggcg tggaagacat
    17881 tatgcatacg ctggcggcaa aaatggggtt tgccctgcgg cataatgtga ttgaagcaca
    17941 tgggctctgt gcggcatgcg tggaagtgga agcgtgtcgt catcctgaac agtgccagca
    18001 tgatcactct gtgcaggtga aaaagaaacc gcgttaagag ggcgtacatc cttgtacacg
    18061 tcgggcagga gggattaatt accagcgata ttcattgcgg gtttcccaat ccacgacctc
    18121 tttttctgcc tgatctttct gataaccata acgttcctgg attttaccga ccagttgatc
    18181 acgtttacct tcaatgatcg tcatatcatc atcggtcagt ttgccccatt gctctttcac
    18241 tttaccttta aactgtttcc agttaccgcc ggcttcatct ttattcataa tcaagacctc
    18301 atcgttaggt tgtgaatgag agtacgttca cttttcttct gaacgtgaga ttaagtgtag
    18361 tcagcaattt ggcttaggat aatttattca gaatttttaa ccgtcacgtt gcggcaaacc
    18421 aggtaccgtt acgccagtga cgccgccaga tagccgccag agaaagcccg cgcaacgcca
    18481 gaaagacggt taatgccagc cacaaaccat gattacccag ccacggcagc gtaaggagcg
    18541 tcagcgcaaa acctgcggcg gccaccgcca tactgttacg catttcggcg gcacgcgttg
    18601 cgcctataaa catgccgtcc agcagataac accagacgcc aaccaacggc aaaatcacct
    18661 gccagataag atagcggtca gccagctgct gaatctgggt taacgacgtc agcaacgcaa
    18721 tgatgtgttc ccctgccagc aaataaacca ccgaaaatag taacgctacg atccccgact
    18781 ggcggcacgc tgcccgccag acatccagca actggctacc gtcgcgcgca ccgtatgcct
    18841 gaccggaatg tgcttcaacc gcgtaggcaa aaccatccag cgcataggcg gtaaaggtga
    18901 gtagcgtcat cagaaccgcg tttacagcga taatgtcact ccccagtcgc gcgccaagta
    18961 cggtgatcgc gccgaaacag agttgcaaca acagcgaacg cagcatgata tcgcggttaa
    19021 gcgccagcaa gcgacggaag tttcctcgcc aggcagtttt cgacatttcg ccggaaattc
    19081 cgcgtagttt gaggatttta cgcaccatta gcagaccaat cagcaatgtt gcatattccg
    19141 caataaccgt cgccagagcc gcgccctgca cgttcatatg cagccccatc actagccaga
    19201 catccagcac aatgttgagg atattgccga ccactaacaa aattactggc gcacgggcat
    19261 attgcacgcc gagtaaccaa ccgagtaata ccagattcgc cagcgacgcc ggtgcgctta
    19321 accagcggat ttcaagaaag cgccgcgcct gttccaggac tgcttcacta ccgccaacaa
    19381 tatgcagagc cagatcgata atcggtgtac gcagcagcgc aattaacgcc ccagccccca
    19441 acgccaacag caacggttgc accagcgcac gggctaatgc ctgaggattt ttggcaccat
    19501 aagcctgcgc agtcagcccg gtggtgctca tgcgtaaaaa cagcaacagc ataaagagaa
    19561 agctggtcgc cgttgcacca accgccacgc cgcccaaata aaccgggcta tcaagatgac
    19621 caattaccgc cgtatcgacc agtcccagca acggaacggt gatattggag aaaatcatgg
    19681 gtaaggcgag atgccagagt gctttatcag atgaagtgag gaatgccatg cagacaagcc
    19741 tgatgaagag agatgaaaaa caaaccgcga cgcctggcgg catcgcggtc tcagagatat
    19801 gttacagcca gtcgccgttg cgaataaccc caaccgccag cccttcaatg gtgaagctct
    19861 gctgacgaag gtcaacgaca attggtttaa actcgctatt ttctggcaac agttcgactt
    19921 tattaccctg ttttttcagg cgcttaacgg taacttcgtc atcaatacgt gcgacaacga
    19981 cctgaccgtt acgtacatcc tgagttttat gcactgccag caagtcacca tccataatgc
    20041 cgatatcttt catcgacatc ccgctgacgc gcagcaggaa atcagcattc ggcttgaaca
    20101 aggaaggatc gacctgataa tgaccttcaa tatgctgctg cgccagaagc ggttcaccgg
    20161 cagccacacg acctaccagc ggcaaccctt cttcctcttc ctgcaacaga cgaatcccgc
    20221 gtgatgcgcc ggaaacaatt tcaataacgc ctttgcgtgc cagcgccttc agatgttctt
    20281 cagccgcgtt tggggaacgg aaccccaaac gctgcgcgat ttccgcacgc gtcggcggca
    20341 tacctgtctg gctgatgtga tcacggatga gatcaaacac ctcttgttgc ctggccgtta
    20401 acgctttcat tccgccccct gggtgtatat acagttatgc tgtgagtata tacagcaaaa
    20461 ggcgattttg gaaccataaa ctgcacaata aaccagagat ttatcgaaat ctggaagggt
    20521 tatccaaaat gcgaccataa caggatgcac caggttatca cggcgacgat aatcgcaatc
    20581 agcaccgcgg cggatcccat atctttagcg cgtccggaaa gctcatggta ttcagagcca
    20641 attcggtcaa ccactgcttc gatggcgcta ttgagaattt ccacaatcat caccagcatc
    20701 acggagctga taagcagcac gcgggtaatt gcgtccacat ccagccagca ggcgatgacc
    20761 accgccaaca ataccgctac gccttcctga cggaatgccg cttcgttgat ccatgcagcg
    20821 cgtaaacctt tccaggaata gccagcagct ttgataattc gggtgaatcc agtggtatta
    20881 ttggccattg aaagaacctt tttacattat gagcgtcaat atcagtgtac cgttaaatcc
    20941 gatgattcgg gtttgcgtaa cgccgcgcga aacatgagcg gataccacag aatttcccat
    21001 gactttctgc tatccttgcc gcgcatttgc attattaacc agaggcttta catcgtttat
    21061 gtccggctgg ccacgaattt actacaaatt actgaattta ccattaagca tcctggtaaa
    21121 aagcaagtct attccggcag atcctgcccc ggaactgggg ctggatacct ctcgtccaat
    21181 tatgtacgtt ttaccgtaca actcgaaagc agatttgctg acgttacgcg cccagtgtct
    21241 ggctcatgac ttgcctgacc cgttagagcc gctagaaatc gacggcacgc tactgccgcg
    21301 ctatgtgttc attcacggcg ggccgcgtgt attcacctat tacacgccga aagaagagtc
    21361 tattaagctg ttccacgact atctcgattt gcaccgtagc aacccaaatc tggatgtgca
    21421 gatggtgcca gtatcggtga tgtttggtcg cgcgccgggg cgtgaaaaag gcgaagtaaa
    21481 cccgccgctg cgtatgctta acggcgtaca gaaatttttc gccgtactgt ggctcggtcg
    21541 cgacagtttt gtgcgtttct cgccgtcagt ttcgctgcgc cgtatggcgg atgaacacgg
    21601 cacggataaa actatcgcac agaaactggc gcgcgtggcg cgtatgcact ttgcccgtca
    21661 acgtctggct gccgtaggcc cacgtctgcc tgctcgtcag gatctgttta ataagctgct
    21721 cgcctcccgc gccattgcca aagcggtaga agatgaagcg cgcagcaaaa aaatctccca
    21781 tgaaaaagcg cagcagaacg cgattgcgct gatggaagag attgcggcga atttctctta
    21841 cgagatgatc cgcctgactg accgcattct gggcttcacc tggaaccgac tttatcaggg
    21901 catcaacgtc cataacgccg agcgcgttcg ccagctggcc cacgacggtc atgagctggt
    21961 gtatgtgcct tgccaccgca gtcacatgga ctacctgcta ctttcttacg tgctgtacca
    22021 ccaggggctg gtgccgccgc atatcgccgc cgggatcaac ctgaacttct ggccggccgg
    22081 gccgattttc cgccgtctgg gggcgttctt tattcgccgt acttttaaag gcaataaact
    22141 ttattccacc gttttccgtg agtatctcgg cgaactgttc agccgtggtt attccgtcga
    22201 gtacttcgtg gaaggcggtc gttcccgcac ggggcgtttg ctggatccga aaaccggtac
    22261 gctgtcgatg accattcagg cgatgctgcg tggcggcacg cgtccgatta cgctgattcc
    22321 gatctatatc ggttatgagc acgtcatgga agtgggtact tacgccaaag aactgcgcgg
    22381 cgcgacgaaa gagaaagaga gcctgccgca gatgctgcgc ggtttaagca agctgcgtaa
    22441 tctcggtcag ggttacgtca acttcggtga accaatgccg ttgatgacct accttaacca
    22501 gcacgtacca gactggcgtg aatctatcga tcccatcgaa gcggtgcgtc cggcctggtt
    22561 aacgccgacg gtcaataata ttgctgccga tctgatggta cgcattaaca acgcaggcgc
    22621 ggcaaacgcc atgaacctgt gttgtactgc gcttctggca tcacgtcagc gctcactcac
    22681 ccgcgagcag ttaaccgagc aactcaactg ctacctggat ctgatgcgca acgtacctta
    22741 ctccacggac tctaccgttc cttcagccag cgccagcgag cttatcgatc acgcgctgca
    22801 aatgaacaag tttgaagtcg agaaagacac tatcggcgac atcatcattc tgccgcgcga
    22861 gcaagcggtg ctgatgacct actatcgcaa caacattgcg catatgttgg tgctgccttc
    22921 gctgatggcg gcaattgtca cccagcatcg ccacatctcc cgcgacgtat tgatggagca
    22981 cgtcaatgtg ctttacccaa tgctgaaagc ggagctgttc ctgcgctggg atcgcgacga
    23041 gttgccggac gttattgatg cgctggcaaa tgagatgcaa cgtcaggggc tgattaccct
    23101 gcaagatgat gagttgcata tcaacccggc gcattctcgc cccctacagc tgctggccgc
    23161 aggcgcgcgc gaaacgctgc aacgttatgc catcaccttc tggttgttga gtgccaaccc
    23221 gtcgatcaac cgcggtacgc tggagaaaga gagccgcacc gttgcgcaac gtctctccgt
    23281 gctgcacggc atcaacgcgc cggagttctt cgacaaggcg gtgttcagtt ctctggtgct
    23341 gacgctgcgt gatgaagggt atatcagcga tagcggcgac gccgaaccgg cagaaacgat
    23401 gaaggtttat cagttgctgg cggagttgat tacatcagac gtgcgtttga cgattgagag
    23461 tgcgacgcag ggcgaagggt aatcagagag aattgccgga tgcggcgaaa acgccttatc
    23521 cggccttcca taatctgcaa attcaataaa ttgcgatgtg ttgtgtaggc ctgagaagcg
    23581 cagcgcatca ggcaacccag aagaaagccg gatgatcatc cggctttttt acatcatcag
    23641 aaatgccagt aactcattgc cagtcctaaa aacagtacca gaccaacata gttattattc
    23701 ataaatgctt taaagcaggc ttcacgctcg cggttggcaa tcagtttttg ttgataaaca
    23761 aacagcgcgc cagccaccag aattgaccag taatatcccc agcctaagcc atttaactca
    23821 ccgatgatcg ccatcagtgc cagtacgcca atctgcaaaa taccaataat caatttatcg
    23881 tattggccga acaggattgc ggtggattta ataccaatct tcacatcatc gtcacggtca
    23941 accattgcat actgcgtgtc gtaagccacc gcccagagaa tattggcgag gaacattaac
    24001 cagcaactca atggcaccga ctcactcaca gcggcaaaag ccattggaat cgaccagcca
    24061 aacgccgcgc ccagcaccac ttgcgggaga tgggtatacc gcttcataaa cgggtacacc
    24121 cacgccaacg ccagagcggc aatcgacaac agaatggtca tcgtattcag cgtcagcacc
    24181 agtaaaaatg aaatcaatac cagcactaca aacagcgcgc gcgcctcttt ctccgttacc
    24241 gcgccgctgg gaagcggtcg gttcgccgtg cgcttaacat gaccatcaaa cttgcggtca
    24301 gcataatcat tcaccacaca tccggcagcg cgcatcagcc agacacccgc gacaaacacc
    24361 gccaggatcc agagctgggg aacgcccggt gtcgccaccc acaacgccca taatgttggc
    24421 cagagcagca gtaacgcgcc aattggctta tccgtacgca ttaagcgatg aaacgccagc
    24481 agcttattct gcgtcagact ccactccata tttttttcct cttagtacaa cggtgacgcc
    24541 ggtaaaaaca gttctgttag caacagcggt ttaccgctta atcgcaggcg ggaacgtcgc
    24601 ccccacagtc cggcatcacg gcctatctca ataaagtccc gggttaatgt cgatgatgtg
    24661 aacagatagc gtcctagcgg cgttttaccc aatttttgta acgccagctc cggcccgctt
    24721 aacgttgaca caggaacgac ggtacgaccg gcaagccacg gttcaccatc ggcacataac
    24781 aaaatttcac gtaaccagta acgagactct ttcggcagca gcggcagttc ttcggggatt
    24841 tcattctgct cgacaaaccc ttcgcggatc atcgtcacgc tcaccgtttt tccctgctgt
    24901 tcaaaacgtt ttgtcatgga atcttccagc aacagccagt cgagcagttg cgaatccagg
    24961 gcagggatct ctttaaaata gcgcagcgca cgcagttgcg ttaacgcggg gtgtgacatg
    25021 ccgaactctc cgttacataa cgtaaaggca ttgtatcgca gaaaagtgtt tacgggagat
    25081 gtaaagcaaa ttaagtgatc taatgcgcaa caaaaacgca acaatcgcat gaagtggtaa
    25141 aacgctgttg aaccttacgc tttgccgatg cccgtgctgg cgagggcaac cgtaggggca
    25201 tcaccgccca tgatgccagg aacggtgatg ttttccttta cttaaatacc tatattcttt
    25261 atacgagtag ctcgccaact cgcagtaaaa attgggaaaa gcgcatattt accatgagat
    25321 ttggagctaa aaagtgtttc ctgagtaaga ttaataggca acatcttatg tctggattta
    25381 tagatacacg tggaattcca attggtatct tttgtaggat tcaacatat
//
LOCUS       DAKISA010000056        25337 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.056, whole genome shotgun sequence.
ACCESSION   DAKISA010000056 DAKISA010000000
VERSION     DAKISA010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25337
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.056"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..1333)
                     /locus_tag="OVW45_002415"
     CDS             complement(<1..1333)
                     /locus_tag="OVW45_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCV5526839.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     AGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNA"
     gene            complement(1379..2137)
                     /locus_tag="OVW45_002416"
     CDS             complement(1379..2137)
                     /locus_tag="OVW45_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5526840.1"
                     /translation="MDALQTQTVNSTTAPQPSYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            complement(2169..2882)
                     /locus_tag="OVW45_002417"
     CDS             complement(2169..2882)
                     /locus_tag="OVW45_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCV5526841.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGLIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIHAALS"
     gene            3056..3748
                     /locus_tag="OVW45_002418"
     CDS             3056..3748
                     /locus_tag="OVW45_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCV5526842.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPRPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(3797..5296)
                     /gene="pitB"
                     /locus_tag="OVW45_002419"
     CDS             complement(3797..5296)
                     /gene="pitB"
                     /locus_tag="OVW45_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCV5526843.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(5588..7447)
                     /gene="gss"
                     /locus_tag="OVW45_002420"
     CDS             complement(5588..7447)
                     /gene="gss"
                     /locus_tag="OVW45_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCV5526844.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYFH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            7652..8518
                     /gene="yghU"
                     /locus_tag="OVW45_002421"
     CDS             7652..8518
                     /gene="yghU"
                     /locus_tag="OVW45_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCV5526845.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(8641..8889)
                     /gene="hybG"
                     /locus_tag="OVW45_002422"
     CDS             complement(8641..8889)
                     /gene="hybG"
                     /locus_tag="OVW45_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCV5526846.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(8902..9243)
                     /gene="hypA"
                     /locus_tag="OVW45_002423"
     CDS             complement(8902..9243)
                     /gene="hypA"
                     /locus_tag="OVW45_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCV5526847.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(9236..9724)
                     /gene="hybE"
                     /locus_tag="OVW45_002424"
     CDS             complement(9236..9724)
                     /gene="hybE"
                     /locus_tag="OVW45_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCV5526848.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(9717..10211)
                     /locus_tag="OVW45_002425"
     CDS             complement(9717..10211)
                     /locus_tag="OVW45_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCV5526849.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(10211..11914)
                     /gene="hybC"
                     /locus_tag="OVW45_002426"
     CDS             complement(10211..11914)
                     /gene="hybC"
                     /locus_tag="OVW45_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCV5526850.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(11911..13089)
                     /gene="hybB"
                     /locus_tag="OVW45_002427"
     CDS             complement(11911..13089)
                     /gene="hybB"
                     /locus_tag="OVW45_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCV5526851.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(13079..14065)
                     /gene="hybA"
                     /locus_tag="OVW45_002428"
     CDS             complement(13079..14065)
                     /gene="hybA"
                     /locus_tag="OVW45_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCV5526852.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(14068..15186)
                     /gene="hybO"
                     /locus_tag="OVW45_002429"
     CDS             complement(14068..15186)
                     /gene="hybO"
                     /locus_tag="OVW45_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCV5526853.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(15375..15662)
                     /locus_tag="OVW45_002430"
     CDS             complement(15375..15662)
                     /locus_tag="OVW45_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCV5526854.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(15781..16668)
                     /locus_tag="OVW45_002431"
     CDS             complement(15781..16668)
                     /locus_tag="OVW45_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCV5526855.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYIMYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            16825..17865
                     /gene="gpr"
                     /locus_tag="OVW45_002432"
     CDS             16825..17865
                     /gene="gpr"
                     /locus_tag="OVW45_002432"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCV5526856.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(17905..18399)
                     /locus_tag="OVW45_002433"
     CDS             complement(17905..18399)
                     /locus_tag="OVW45_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCV5526857.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            18590..19474
                     /gene="yghA"
                     /locus_tag="OVW45_002434"
     CDS             18590..19474
                     /gene="yghA"
                     /locus_tag="OVW45_002434"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCV5526858.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(19634..20059)
                     /gene="exbD"
                     /locus_tag="OVW45_002435"
     CDS             complement(19634..20059)
                     /gene="exbD"
                     /locus_tag="OVW45_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCV5526859.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(20066..20800)
                     /gene="exbB"
                     /locus_tag="OVW45_002436"
     CDS             complement(20066..20800)
                     /gene="exbB"
                     /locus_tag="OVW45_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCV5526860.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            21052..22239
                     /gene="metC"
                     /locus_tag="OVW45_002437"
     CDS             21052..22239
                     /gene="metC"
                     /locus_tag="OVW45_002437"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCV5526861.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            22379..23038
                     /locus_tag="OVW45_002438"
     CDS             22379..23038
                     /locus_tag="OVW45_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCV5526862.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(23078..23977)
                     /gene="yqhC"
                     /locus_tag="OVW45_002439"
     CDS             complement(23078..23977)
                     /gene="yqhC"
                     /locus_tag="OVW45_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCV5526863.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVDYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            24171..25334
                     /gene="yqhD"
                     /locus_tag="OVW45_002440"
     CDS             24171..25334
                     /gene="yqhD"
                     /locus_tag="OVW45_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCV5526864.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKI
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
BASE COUNT         6336 a         6758 c         6305 g         5938 t
ORIGIN      
        1 ctgcgttata aagcaaggaa tgacgctgaa aaaatgcctt taaagttgga attaacgaga
       61 gcaccacatc cagacattgc ccaccgaagt aagcaaacgc tgctcccagc acaccaaaca
      121 gatgacagaa catgaacagc aatacgatgt agccgattga ggcgatggtt tgcgccacga
      181 gaaaagcgcg ctgttttccc gccatcaata gcaatgactc ctgcgggaaa cccagcatcg
      241 aaatcacaat tgcgcccagc atcacctgaa tcaggtcata cgcttcgaga tacttaacgc
      301 caaacaccag cgaaatgagc ggcttgccgg cgatcaacac cgcgagcgcc accagaatac
      361 cgattccgcc cgccagtaaa ccggacttca cacctaacaa ccacggtctg gtggtgcgcg
      421 gatctaaacg catcacctct gggtaaaaac ttttacccag caaacccgct ggcgttccgg
      481 cggcgtcgaa gaatgtcatg gcgattttaa ataacccggc ggcagcgggt cctaacacga
      541 tccccaccag cactgtgctg cacgagttac gcgccgacca gatggagtgg gcaatgttgg
      601 ttgaccagac aaaactccac gcgcctttga tgtgtcgggc agactcaaac agattcaatt
      661 tgaaggcatt atggatattt ctgcggcgta attcgcgcgc tgcaaaccac cagtacatgg
      721 tgccgccaac cagattcgac acgtaccagg caataacaaa acccgcaaaa ccaaagtcaa
      781 aataccaggc tacgacgctc cccgctgcgc gcagaaaagg tttcgtcgcc tgctgtacag
      841 caattaaatc gaagcgatct accgcacgca gaatgccggt cggcgtggag gaagccattg
      901 aaggaatgag cgtgcaatac agcgccgcca gccaaaagct ttggtcatcc agacctaatg
      961 aatgggaaag gaatggcagt aaggcaatgc cgccgacaat cgccaccgcg ccgctgacga
     1021 tatccagcga gaaggaaaat gagacgacat tgcgaaattg ctgcggatta ttgttggtca
     1081 aggctggcgt tccgtactga accaccagct gccatgtctg aaacttaata aaatcgctga
     1141 tcgacttggc gtacgattgc acaatcacca gtacgccaaa catggcgggc gtcatccctt
     1201 taccggcaca cgagagcgcc aacagcccca gcaaggcgct cacgacatta ctggagccta
     1261 accaggcgct attgcgaata atggtgcgaa acgcgccatc tgcaaaccaa tgtttgatgt
     1321 taaaacccgc caattcagcc tgaccttatc gttgatagta aaaagtatcc cgccagcctt
     1381 aagttaaact tcggcggtca gaaacgatgg caaccagaga aaccgccttc tgtgcctgtt
     1441 ccagcacttc gctgtagggc gctctggaat caatctcaag aatttttgtg ccgttatagc
     1501 caatcttcga catgacaccg attttgtcct gcagctcggc atagtcatgg tcaggcttgc
     1561 gggagatggc agtctcaata tcaatgccca ggcgaataat taattccggg cgatattgcg
     1621 ccatttgttg gtataaacgc cgttcgcgct gcgccagaaa catgctgatt ttcccggtcg
     1681 cacgttcgac gccaatcccc ggtccgtcgt aataaaagcc cgaaatttca gcctgcggga
     1741 agcgatcgct gaccaccaga acgccacttt gcgccagtcg ctgaaccttg cgtagattcg
     1801 ccattcttcg cagcgagaag cagtacataa tcaccgccgc ccatagcgcc ggagatttgg
     1861 ttttcatgct ttgggttttc gaggatttgg ccgccagtcg ccgttccagc cagacgccaa
     1921 ccaacggtaa tcgtttgatt ttgtcgccgt cttcgcccga gagcagcccc agatagcgcc
     1981 gctcggtttg ccagtgctgt tgcaatgatt tcaccagatc ggtggtcagc gtggatttac
     2041 cggtgccatc gcacccgacc accgcaatta gccccggaat gtagctgggc tgcggcgcgg
     2101 ttgtactatt aacagtttgt gtttgtagtg catccattcg ttaaatcatc ccctatccat
     2161 tttggtgatc aggaaagcgc ggcgtgaatt gcacgtagtg acgcttgcag gatttcatcg
     2221 gctggttgcc gaccatccag ttcaaggatc ctggcgccgt tgaaagtcag ttgcggcgta
     2281 acggcgattt tttcctgtaa cgctgccagt tggtggtcag gtttacgcgc aaacgcggtt
     2341 tgttcatcaa tgccaagacg aatcaacaat acgggcaaat aagatgccat ccattggtac
     2401 agcttcagct cgcgctgcct taacattttt atccaaccgt taccgcccgt ggtttttgcc
     2461 aattgcgggc catcaaagcg aaaacccggc acttcaacct gcgggtagcg gtcggtgatg
     2521 agcagaaagc cttgctggct tttacacaac attttgcgaa acttgtacgc ccgccagcag
     2581 gaaagcagat agataaccag agcagtaata ttgccaggcg gtgttgaggg tttttcgtgc
     2641 acatgtgccg ctttacttcg cagataacgc ccaaaaggtg cgccaataac agggagctgt
     2701 gaaatccatt cgccaattag cccggacgat tgcccgagat aaatgtgttc tgttggcatt
     2761 cttgctgcca gttcatttac caggcttgcc gtgagggtcg atttacctga accatcacat
     2821 cctacaatgg caataacacg cactggcgtg gaattaatta ttgacatatt acgttgattc
     2881 acgaaaaacc cggcagtaaa taatgtattg aatattacgt ggtccgtgct cagaatatcc
     2941 gttcaacctt attgtgacaa tggttaattg tgacattgac ctgaattacg ctttcataaa
     3001 aacatattaa ccaaataaat atttttaatg gatatttaaa ttaaaggata tattcatgca
     3061 gtcaataaca cctccattaa ttgccgttat tggtagcgat ggttcaggca agtcaacggt
     3121 gtgtgaacat cttattaccg ttgtcgaaaa atatggtgct gccgaaagag ttcatttagg
     3181 aaaacaggcc ggaaatgtcg gtcgtgcagt gacaaaatta ccgttgatgg gaaaatcctt
     3241 acataaaaca attgaacgaa atcaggtgaa aacagcaaaa aaattgccta gaccagttcc
     3301 ggcgctggta attacagcgt ttgtcgcccg tcgcttactg cgctttcgtc atatgcttgc
     3361 ctgtcgtcgt cgcgggttaa ttgttctaac cgaccgttat cctcaggacc aaattcctgg
     3421 cgcttacgat ggtacggtgt tcccacctaa cgttgaaggt ggtcgttttg tctcatggct
     3481 ggcaagccag gaacgtaaag cgtttcactg gatggcgagc cataagcctg atctggtcat
     3541 caaactcaat gttgaccttg aagttgcctg tgcacgtaaa cccgaccata aacgggaatc
     3601 gctggcgagg aagattgcca taacgccaca gttaaccttt ggtggtgcac aactggttga
     3661 tatcgatgcc aatcagccac tggaacaggt gttggttgat gcagaaaaag cgattacgga
     3721 ttttatgacc gcgcgtggtt atcactagtc aaaaatggaa atgcccgatc gccaggaccg
     3781 ggcattttca ggaaggttaa atcaactgca atgctatcca gtacagccca ccagaaagaa
     3841 aaattgccgc cggtaaagta aacacccacg ccatcaggat gctggttacc gttttacgct
     3901 gtaacccacc gccgtccacc accatcgtcc ctgctaccgc agacgagagg acgtgtgttg
     3961 tggagacagg catcccaata taactggcaa ggccgataga cactgccgcc gtcatttgtg
     4021 ccgccatgcc ttgcgcatac gtcatgccgc gcttaccaat cttctcaccg atggtcatcg
     4081 ctacacgacg ccagccaatc atggtgccaa tgccgagcgc cagtgctacc gccatgatga
     4141 tccacaccgg agcgtactca atggtgctta acatatcgct gcgcagtttt ttcagcaggt
     4201 tctggtcttc tttactgacg cctggcagtt tcgccagctt cgcggaggta tcagagatgc
     4261 acagcataat gcggcgcagc tggctgcgct gactcacgct taacggctcg taactttcca
     4321 tattgcctgg cagcatcgtt ttaacgcgcg caatcgcatc aaaggtattt gccggatgac
     4381 agtgaaactc tgttacttgc gtgccatcag tcgatgctgc aggcaatgga ggttccatcg
     4441 caatcaactt ctgcggcagt tcaggatgct gttgcaggta gtgttcgaag ttggtgacgg
     4501 catcgcgggt acgggtaatt tcatagccgg acgcgttcat attgacgacg aagccagcag
     4561 gggcaatccc caccagtacc agcatcacca ggccgatccc tttttgtccg tcgttcgcgc
     4621 cgtgcgaaaa cgccacgccc gcagcggaaa caatcagcgc aatacgcgtc cagaatggcg
     4681 gtttacgttt gccttttttc tttttgcgat cttccggaat gcggtgaata cggtcacgct
     4741 ttttcgtccc gctccagtag cgtcgcagca ggaatatcag gcctcccgca atgaccaggc
     4801 cgacgatagg ggaaacaatc agcgaggaga aaattttggt cacttcacgc aggtttaacg
     4861 catccatcac cgatgagccg gttaacagcg cgttggttaa accgatgccg ataatcgcac
     4921 caatcaaggt gtgcgaactg gaggccggta aaccgaagaa ccacgttccc aggttccaga
     4981 taatcgccgc cagcagcatg gaaaagacca tcgccaggcc gtgggttgac cccatattca
     5041 gcaacaaatc ggttggcaac atatggacaa tggcataggc aacgctaagt ccgcccaata
     5101 acacgccaaa aaagttaaaa aatgccgcca tcaccacagc aagttgtggt tgcatggcac
     5161 gagtataaat aacggttgcc accgcattcg ccgtgtcatg aaaaccattg attgcttcgt
     5221 agaacaacac aaatgccaga gcaagcaata acaaaagccc tgtgtatata tcaaggccaa
     5281 caaataaatt tagcatatat tagattacgc cattttgaat ttacgaacgg acgcattatc
     5341 agtgacttta acggcatggg caaagtgaaa tatcattttt taacgataaa aagagtgtta
     5401 tttcttgtgt atcagacaat aagcattcat tcaaaacctt tagaaataat caaaacacag
     5461 gtattccact ggttaatata ttcacatatg aaataaataa atattagaac atataaatat
     5521 tgaatatttt gattaatccc cgtactgatt attcttcata atcagtacgg ggttgctaca
     5581 acatgtatta ctttttcacc acaattaacg gttcaatatc actctctttt ttgatgacca
     5641 gtgattcatc accgcgcaaa cacgtcccac cgtagttgcc gcccacggtg aaggtacata
     5701 cctgaatgta tttaccgtcc actttcggca aacaccacag ttgctgatag atgtttttct
     5761 gctcggcaaa tttaccgctg gttttgtcca gcacctcttc atgatggctg accaggtcga
     5821 tattgctacc acagcgaccg gcaatcggtt tcactgcgta acctgttttc accaattcat
     5881 cattaacagt gaaatcggta tccagcaggt aacgatggtg cgggaacagc gaccagagga
     5941 tcggcagaat cgctttgttg ccggggatca ccgtccacag cggctcaaag actagcactt
     6001 ccgggcgcag caatacgtcg ataagacgca cttcgttttg cggatgaccg gtacggattg
     6061 gcaccgcagc aaactcacgg tcgctaactt cacgaatctg atcaaacgcg gtttcccacg
     6121 cccaggtttt ccacacgcag ttaaccagtc gcccttcccc atcaatcagt tgcccggcag
     6181 catcccagcc cagttcatcc aatccacgca agatacgcgt ttcaaagccc gcctggtgca
     6241 gcgcctgctc cataaactgc gcgtgatagt tttcctcgat atctttgtcc tgcatgatat
     6301 ggacaaacgg acgtgcacga ctgtgtttcc aggcaccagc caattcgtta atcagccctt
     6361 ccgccggatt gaagccgttg cctttatagc cctgctccgc ccaacgttcg aggatcaagc
     6421 ccgcttcggt atgacaggag gcggagtcgg cgttgtactc gtaaaccttc aggccacgct
     6481 catccatgca gaaatccata cgaccagtga tcatatggtg acggcgacgc tgccaggaga
     6541 gacgcaaacg tggccagagg attttcggga tgtcgaacag cgccagcagg ttgtcatctt
     6601 tcagcacctt gtcggttgcg tgaaaataca tcaggtgcag ctcgttggtg gctttaatta
     6661 gctcctgctc ggcactctcg gtaatggtgt agtaatgata aggatcctga ttgatcacct
     6721 gaccgttggc ctgcacatag gcgttttgca gcggatcttt ttcatccagc catttaccgt
     6781 caaactggcc tttgttttcc aggcgcgctc cgctgatttt cagcagctcg cctgcaattt
     6841 ccggctgcgg taagctgtat tcagtatctt ccgtctggat catccagccc agaatggtgg
     6901 tgtcatcaaa agtgtctttc agggtatagc agccgttttc gaccaccatc tccagctcac
     6961 gcgtccactg ttgcccttgc ggcaacgggg aatgaatcac gttctgttcc gcaatacgga
     7021 ctttgttgcc atgcaattgg gtaatgatgg cgacatggcc agtgtcttta aattcaccgc
     7081 ctttatccca gataagaagc gcacccgcga ccggcgcacg cggcgagccg ttaggaaatg
     7141 cctgcaatgg caggatgttg tcattaacca cttctcgcag gaagcgcagc gagaaaatct
     7201 cccacgccat acccacgtca gtaaagacca caccgtaatt cagaaagaga aaacggcgag
     7261 caaattcaac gcattgccac ttgtggccca tatattcgtc gtcgatatag ctacggaata
     7321 cggcgtcatc ttcgtattcc tgcggatcga gagaactgta atctgaagag tagattgcta
     7381 ccccacctgg ggcgtagccc aataatgtcc cgaacggggc atcctggctg gtcgttcctt
     7441 tgctcatcac tttaccttta acaatacaac ctaagcagtt ggcgtaattg ttgtgctctg
     7501 attacctgct cagcgacatt aaccggacag aggtcagact aatcatacac ctcatcgcag
     7561 cggctggcgc aaagggttaa aaaattcaca ttctgtacag caagtgacct gctacactgc
     7621 ttcaacacta ccactcagaa ggcaactcac tatgacagac aatacttatc agcccgcgaa
     7681 agtctggacg tgggataaat ccgctggcgg cgcgttcgcc aatatcaatc gcccggtttc
     7741 tggtccgacg catgaaaaaa cgctgcccgt tggcaaacac ccattgcaac tttattcgct
     7801 gggaacgccg aacggtcaga aagtaacgat tatgcttgag gagctgctgg cgctgggcgt
     7861 tactggtgca gagtacgacg cctggctgat tcgtattggc gatggcgatc aattctccag
     7921 cggctttgtc gaagtgaacc caaactcgaa gatcccggcg ctgcgcgatc atacgcataa
     7981 tccgccgatc cgcgtgtttg aatctggttc gatcctgctt tatctggcgg agaaatttgg
     8041 ctacttcctg ccgcaggatt tggcaaagcg tactgaaacg atgaactggc tgttctggtt
     8101 acagggcgcg gcaccgttcc tcggcggtgg ttttggtcac ttttaccatt acgcgccgat
     8161 gaaaattgag tacgccatca accgctttac catggaagcc aaacgtctgc tcgacgtgct
     8221 ggataagcaa ctggcgcagc ataagtttgt tgcgggcgat gagtacacca ttgcggatat
     8281 ggcgatttgg ccgtggtttg gcaatgtggt gttaggcggc gtgtatgatg ccgcagagtt
     8341 tcttgatgcg ggcagttata agcatgtaca acgctgggcg aaagaagtag gcgaacgtcc
     8401 ggcggtgaaa cgtgggcgta ttgttaaccg caccaacgga ccgctgaatg agcagttgca
     8461 tgagcgccat gacgccagtg atttcgagac gaatacggaa gataagcgtc aggggtaagg
     8521 gttggtgttc gtcgcagcaa gccatccagg ccggataagg cgttcacgcc gcatccggca
     8581 atcgtgcaca atgcctgatg cgacgctgcc gcgtcttatc aggcctacga aagctaatca
     8641 tcacgcgctg gtaatgtcgt aatccatttg gcgcagtgcg tctaatgtgg ctttggcttc
     8701 atcttcgtcg atgatgctca tggcaaatcc gacgtgtacc agcacccact ggcccagtag
     8761 atcggcaggg ttaccttcac aaatcagggc gatattcacg tcgcgcttga taccacatac
     8821 ttcaacctgc gcaagctggt gaatatcttc accgacagcc agcacctggc ctggaacgcc
     8881 aatacacatt attaactccg gttattcaac ttcaatactt ttgacgatca gcgaatcgcc
     8941 ggtatcgaca cgcaaccgct cgccgtgaca gagcggacac tgcgcatcgt gctgatgaat
     9001 ctccaccacc tggctgcaat cccagcacca agcctgggcg ggtttataga cgatatgtaa
     9061 atcgcaccct tgcgccaccg ttccgtggca gacaatttca aaactaaaac ggacggcgct
     9121 ctcctcaacg caggagagcg cgccaatttc cagccacacg gcggtgacgc gcttaacatc
     9181 gtgctgctcc gcctgccgtt ggataatttc aacggcgctc tggcaaagag acaactcatg
     9241 cattttcgcc actcctgcga ccaaacagca gggcgcgacg ccctgcgtgt ggtacatccg
     9301 gattcgtgac tggcagcgaa aggatcattc gtgcgcagtc atccgtcagg cgttggccct
     9361 cttcaatcga catgctgtgc gaaagcggcg acatcagcga acaggagaga tattgcgaaa
     9421 caccgtccag ttcgccaacg gtaaaggtca tagtgccata cggcagttgc agaccaattt
     9481 tttcactcac tttgcgcagc ggccagagtt gatccgggcc ggggaagata actgcactca
     9541 gcatccacgg ggtgatcaca caccccgtcc actgaccttc gaacagcgta aaatcagaaa
     9601 catacaccgg cattgaagga tgcagaaaag agagatcgtg catcgaacgc cgggcaattt
     9661 cttcaaacgc tgcctgtact tgcgccttcg gggaggtctg gaaacctgct atctcttcag
     9721 tcatgaatcg cctcccgtgg gatggcttcc acgcctgatt cgcgcagcgc agccagaacc
     9781 tgctcaagcg caggttcaat cattgcttca accgtcggcg ttaagccgat gtgtggctcc
     9841 agcgattccg ggatcacgcc gaccagggtc agctttttcg gaaactcacc ggtgaagcgc
     9901 agggccgaca atacgtcggc caggccaagc tgatgcggag agattttgtt ggtaaacaac
     9961 gccggaactt cttcatcccg caggatcatc atcgttcccg gcgtgttctt tttcgacaca
    10021 atggcatccg caataatcag atgatcgcga tttgccatgt cgccaagcag ctccattccc
    10081 gccgtgccgc catcgaggat ctcaacataa tccggcagaa tgtatcgttg ctctaacgct
    10141 tcgacaatcc gcacaccgat ggcttcatcg gtcagcaaaa tattgccgac ccctaagact
    10201 aaaatacgca ttacagaacc ttcactgaaa ccacttcgtt gccgtcggca tccactacgt
    10261 gtaccgcaca ggccatgcac gggtcgaagg agtgaatggt acgcaccact tccagcggtt
    10321 tattcggatc agcaaccggt gtacccacca gcgactgctc gtaaggaccg acgtcatcat
    10381 tgaagttacg cggaccagag ttccaggttg atggaacaac tgcctggtag ttactgataa
    10441 taccgtcttt aataaccatc cagtgagaga gcataccgcg cggcgcttcg aggaagccaa
    10501 cacctttgaa ctcacccgtt gccggaatgt tcggtttcac aaaggtggtg tgatcgcctt
    10561 tgccgatatt ggtgatcagt gcactgtatt gattttgcag gatatcctgc aattcgcagc
    10621 agtgaacggt acgaccaata atacggccca gcgtggagtg cagctgcgcc acttccagcg
    10681 tgttgccagt cagtttctga taaatcgcaa cgatttcatt cagtttgttc tgggtagatt
    10741 cgcgacctgc cgccagtttc accagcatat tagccagcgg acccacttct accgttttgc
    10801 cgtagaaagt cggtgatttc acccaggaat atttcccgtc gtcagaccaa ccatcataag
    10861 ccggaatggt ggtaccttcc cacggtgcct gcggcgcttc gtctttatac caggagtgct
    10921 tcgcgctttc ctgaatgcct ttgatcaggt attcatcgga atgagaagtg atcggacgat
    10981 acgaggacag atccgcattc tcaatgtagc cgcccgggaa caggaagctg ccgtttttac
    11041 tgtcggtcgg gaattccggc acgctcaggt agttcaccgc acctttaccg cgcgtcagcc
    11101 attccgggta gaacgcggca ataactgcgg tatcaacctt ataaacctgt tcaacaaagt
    11161 cgctcagctt gtcgatgaaa gacttgatgt acatcaggcg ctcaaggttc agcacgccca
    11221 aaccgtcgag gttgatcggg ttcgcgacac cacctaccgc caggttctga atgtgcggcg
    11281 ttttaccgcc cagcagcgcc acgacgcggt tagcgtcacg ctggcactcc agcgcctgca
    11341 ggtagtgcgc taccgcaatc aggttcactt ccggcggcag tttcatcgcc gggtgacccc
    11401 agtagccatt ggcgaaaata cccaactgac cgctggcaac cagatctttg atcttgttct
    11461 gaactttagt gaactcttcc ggactgttca gatgccaggt cgaaacgcct ttcagcattt
    11521 cggaggcttt ggttgggtca gcttgcagtg cagaagtgat gtccacccag tccagcgccg
    11581 aaagctgata gaaatgaacg atatggtcat gcgtggtgtg cgcagccaga atgatgttac
    11641 ggatgtattg cgcgttaacc ggaacgtcga tattcagcgc actttctgcc gcacgaacgg
    11701 aagacagcgc gtgagtggta gtacatacgc cacagatacg ttgcacaatc atccatgcat
    11761 cgcgcggatc gcggtttttc acgatctctt ccatgccgcg ccacatggta ccggaagccc
    11821 atgctttcga aacgacgcca ttttcgattt cgcaatcgat gcgtaaatgc ccctcaatac
    11881 gggttaccgg atcaatagta attctctggc tcatgctttg ctcgcctcat gacgattatg
    11941 atcgttttgt tttaaaggag gaagtatcgg cagtagacga atgagtacga tgtaagcgca
    12001 aatctcaata gccacaaaac caatagaaat caacagttct tcccaggtcg ggaagtaggc
    12061 gtaaccgccg cccgggttga atgccaccag cgaataggtc agacgccagg ttgcacaacc
    12121 taacagtgcg ctcagtgctg acaggaacag catgcgggaa tcattacgca gcttcgccac
    12181 acgcagaacg accagcggga agagcatcag caggacttca atccagaaca tcacggagta
    12241 gaagtcaccg gcaaacgcta acgacagctt gtcgcgatag ataagctcgc caaagcgcag
    12301 cacgatgaaa atcgccagca acacactgat ggtgttggtc agcttgacga acagactctt
    12361 ttcatccgga ccgttgccac gcagacccgc ctgcaccagc gaaccttcaa agatgacaat
    12421 cgagaagccc atgatgaacg ccgtcagcag cgagaacagc ggcaacattt catagctctg
    12481 ccacaacgga tgcaccttgt agcccgccga gatcatcagt gaccccattg aagactggtg
    12541 catggtcggc aacagcgcgc caagtgcaat gatgaagaac ataaccttgt tcagacgctt
    12601 gagagaaacc ttccagccca gacgttcaaa cagtgccgga gcaaactcca gtgccatcac
    12661 gccgatatag atggtcatac agaccgccgt ctcgaacagt accgagttca cgttgaagtg
    12721 acccggaatg tagaagtacg gcaggttcca gtagcgaccc acgtcgatag tgatcgacaa
    12781 gccacccagt gagtaaccaa acagactcgc caacagcgcc ggacgcacca gcggatggta
    12841 ttgcccacgg ttaaagacgt ataccgccca cgccagcgcc cagccgccac aggcaaagcc
    12901 ggtgccaatc agcaggtcaa acgcgatcca cacgccccac gggaagccgc cgttcaggtc
    12961 agagacagag cccagaccga acaccagacg cttcacaatc aggagcatac agatgacgat
    13021 taacggtcca aaaatcatga ccggtttact gatgattttg ccgcccagcg gttgtggatc
    13081 atgactcatg atcgtctcct ccgtcgtgat ggtcgttttt ggtgttgcga cgaaccagca
    13141 cggttaagcc cgccagcaca gccagtggta gcatcatgcc tttatacagg gtgtgttgaa
    13201 tattttcgga acgcgcaccg gtagaaagat cgtccagttt cggcaggtcg agattttcat
    13261 aaggcacacc cgtcagtacc agaacctgag taccgccgcc ctctttctcg ccgtacagat
    13321 gcggataata tttcggcacc gtatgcaggt aagtgtcgcc agatttcagc gtctgacgcg
    13381 gatagtggta ttcgctgcca ggcttcagcg ccagacgttt tttcgcctcc gccatcagct
    13441 cttcacgcgt accgaaaatc accgcgcccg ccgggcacac ttctacgcag ccaggcagac
    13501 cgcctttatc gagacgttcc acacctttct ggttgcacag ctcgcactta tgcagcgcac
    13561 caaacgggtt gttgtagtcg tacttcggca cgttgtacgg acaggcgacc atgcagtaac
    13621 ggcaaccggt gcacacgtct ttgtcgtaat ggacaatgcc ggttttcgga tcttttttca
    13681 gtgcagagac cgggcacaca gagacacagt tcggatcgac gcagtgcata cactgtttct
    13741 taatgtacgc gtagccgttc tcctcctggt ctttgttgac ccctgtgccg ctggtccaca
    13801 cctgaatgat gttattggta tacggcgaca gtttgtcgtt gttcgaccag gtctgttccc
    13861 cttgcgggtt acgttcaggg aagttgatat cctgacactt ggtgacgcaa gcctggcagc
    13921 ctacgcacaa ggtcgagtcg tacaacatcc ccagcgatcc cggaattggc gggcggtttt
    13981 cagcagccgc atgactgaca gacggcagcg cgcccgtcag caatgccccg caggaggctg
    14041 ctttaataaa attacgtctg ttcacggtta ttctccccgt gagtcagcgt tatctttctt
    14101 ttgctgacga cccagttcac gcaccgccat cacgctgaca ccggcaacca gcccaaccac
    14161 accgccgagc aaaccaatag cgcctgcaga gacgttgccg ccctctttag cgttaacatc
    14221 cggtttctgt gaacgcggag tctgattttc gacgttggca agctgatgga tgcctttatg
    14281 gaagccgata ccttcttcgt tacagccata gcatgggtga ccaatcgcta ccggccacac
    14341 gccgccaaca tcgcagaatt gcagcgttga gcagttgccg taagtttctg gccctttaca
    14401 gccgaggtgg tacaggcacc agccttcacg gtggccttca tcaccgaact ctttggcaaa
    14461 acgaccagca tcgaagtgcg ggcgacgttc gcagtgttcg tgaatcagac ggccataggc
    14521 gaaggtcgga cggtttttgt catccagttt cggcggtttg ccgtaagtga tgatgtgcgc
    14581 aacggtcgcg aggaagttgt gcgggttcgg cgggcagccc ggaatattga taacggtttt
    14641 gcctggcaga acttcttgca ggctgactgc gccagttggg ttaactccag ctgcggcaac
    14701 accgccccac gcagagcagg aaccgatagc gataatcgct gctgcgcctt ctgccgcttt
    14761 gcggatgtga tccacaatcg gctcaccagc aaccatgcaa taaataccgt tatcttttaa
    14821 tgggatggaa ccatccacaa ccaacacata ctgccctttg tacttctcaa gtgcgttatg
    14881 tttgttctct tcgacctgat gaccgaaggc ggcggaaagc acttcgtgat actccagaga
    14941 gatagtctcc agcacgaggt tttctaccgt tggatgcgtt gcacgaagca gagattccgt
    15001 acaaccggtg cactcctgcg cgccaatcca gataactggc ggacgctgcg ggttagtaac
    15061 cgattcggcc atctctgcag cggctttgct acttaacccc atggtggcgg ctaatgctgc
    15121 acaaagcttc atgaaatcac gacggttaat gccgtgagaa tggatgaggg tgttatctcc
    15181 agtcatttat agttattccg ttgcgaagac ctggcatata ttttgcctca atcgcaaaat
    15241 caataatgcg atcgatgcgc catttaccac acatttatta tggttatcga tatcatgata
    15301 ctgcggcggg gcgctaaaac gaagggaaga agcattagtg taattaatta aagcaagata
    15361 atacgtatgt ttgatcaatt ttcgaggcga taaccggcca taaaaaagcg caacgtagaa
    15421 caggaattat tctcacgaaa gaaatccatt accatctctt tatccagtcc atagcggcgc
    15481 gtcagaatac ccgcttccca ggcgctaagc tggcgatctc cggtcttttc gtacatccgg
    15541 tgtgagtagc ctaatacaaa accgcgttta taatcggcac agaattttgt cacattaacc
    15601 gcactgtcgg catgcgttgc ttttaatccc gccattaagc ctttaccaaa atggttattc
    15661 atcgttcacc tcattcgcta tatatcgcca tatatagcga tattttaggc aaagaacgac
    15721 ttttgcgcac gctatttttt catgcttgtt taagacgtaa tccagtaaga gataaaccta
    15781 tcaggagaga tatttatcga accatttcag tgtcctttgc caggcaagat cggcggcaga
    15841 tttgtcataa cggggcgtgg aatcattatg gaatccgtga ttaacccccg gatagatata
    15901 cgcctcataa accttattat tggctttcaa cgccgcctcg taagcaggcc agccctcgtt
    15961 gattcgggtg tccagttccg cgaagtggag tagtaaaggc gcttcaatct tcgccacatc
    16021 ggcagtgggt gcctgacgac cataaaacgg caccgcgcag gccagttccg gatacgcgac
    16081 agccgccgcg ttcgatacgc caccgccata gcaaaatccg gtaataccca ctttgcctgc
    16141 cgcttgtgga tagcgttgca taaactcaat tgcggcaaag aaatcattca tcagtttggt
    16201 tggatcaacc tgctgttgca gctcacgacc tttatcatca tttcccggat aacctccaac
    16261 ggaacttaag ccgtcaggtg ccagggcgat ataccccgcc ttcgccactc gccgtgccac
    16321 atcttcgata tacggattca gtccacgatt ctcatgcacc accaccacgg ctggcgtttt
    16381 gccgctcatt tttgcgggtt tcaccagata accccgtacc tcgccgtgac catttggcga
    16441 aggatacatg atgtactcag caacaatctc cgggtcggta aactctacct gagtcgccag
    16501 cgcataattt ggcttgagca aatcaaacaa cgctaatgcc gtcatcccgc ccaccgcata
    16561 cttcgccgca aggttgagga actcacgttt cgaaattttc ccgtgagcgt aatagtcgta
    16621 gtaatccaac aactcttgtg ggaaatcttt ggcggttaaa cgcggcatcg ttgcactcct
    16681 cagttggtgt ttttttaagc aaagtataag cacgtgtttt tgcccagttt ttcgtcactc
    16741 tgtgagccag actacgggat acgcgctggc gaatcgctaa actagaaaca ttgtttcgaa
    16801 attgaacggt ggaaaggaga ggtcatggtc tggttagcga atcccgaacg ttacgggcag
    16861 atgcaatacc gctattgcgg aaaaagcggt ttacgcctgc ccgcgttatc gctcggttta
    16921 tggcacaatt tcggtcacgt taacgcgctg gaatcacagc gtgcgatcct gcgtaaagcg
    16981 tttgatttgg gcattacgca ctttgattta gccaacaatt acgggccgcc tccaggaagc
    17041 gcagaagaga actttggtcg cctgctgcgg gaggattttg ccgcttatcg cgatgaactg
    17101 attatctcta ccaaggctgg ctacgatatg tggcccggcc cttacggctc tggcggttca
    17161 cgtaaatacc tgctcgccag cctcgaccaa agcctgaagc gtatggggct tgagtatgtc
    17221 gatatctttt actctcatcg cgtcgatgaa aatacgccga tggaagaaac cgcctctgcg
    17281 ctggctcatg cggtacaaag cggtaaggcg ctgtatgtcg ggatctcctc ttactcgcca
    17341 gagcggacgc aaaaaatggt cgagttgctg cacgagtgga aaattccgct tttaattcat
    17401 caaccttcgt acaatttact gaaccgctgg gtggataaaa gcggcctgct ggataccctg
    17461 caaaataacg gcgtgggctg tattgccttt actcctctgg ctcagggatt gctgaccgga
    17521 aaatatctca acggcattcc gcaagattca cggatgcatc gtgaagggaa taaagttcgt
    17581 ggtctgacgc cgaaaatgct taccgaagcc aacctcaaca gcctgcgctt attgaatgaa
    17641 atggcacagc agcgtggaca atcaatggcg caaatggcgt taagctggtt gctgaaagat
    17701 gatcgcgtga cgtcggtatt gattggtgcc agccgcgcgg agcaactaga ggagaacgtg
    17761 caggcgctga ataatctgac atttagcacc gaggagctgg cgcagattga tcagcatatc
    17821 gccgatggcg agctgaatct gtggcaggcg tcgtccgata aatgacctgt taataacggg
    17881 cgcgcgaacc gcgcccgaat cagatcagtg attatgacga gtcagtcggt caagatagcc
    17941 catcacaaat gcagaaagca caaacgtcag atggataatg acgtaccaca tcagtttgtt
    18001 atccgggaca tttttcgcat ccataaagac gcgcagtaag tgaatggaag aaattgccac
    18061 aatcgacgct gctactttgt ttttcagcga cgttgcgtcc attttcccca gccagttcag
    18121 cttctctttg ttctcggaga tatccagctg tgagacgaaa ttctcataac cggaaaacat
    18181 caccatcacc agcaaaccgc caaccagcgt catatccacc agcgacagca acacgaggat
    18241 caaatctgat tccgccatcg agaagatatt cggcagtacg tgaataatct cctggaagaa
    18301 cttcagcgcc agggcaacta acgccagcga aaggccaaag tacacggggg caagcagcca
    18361 gcgagaagca tacattgcat tttcaagaaa acgttccata cattcctgtc gtgtagttaa
    18421 aacgggcaac agtatatcgc aattgagtaa acagcacgca acagcaaatc aacatatacg
    18481 ttaaaccttt gtcgcataat tctgaggttt agggatcttc ctggttccct tatcaacgca
    18541 tcgagagcgt ctacccttaa aggacgttga acctgaaggg agaaaaacga tgtctcattt
    18601 aaaagacccg accacgcagt attacactgg tgaatatccc aaacagaaac aaccgacgcc
    18661 aggcatccag gcgaagatga caccggtacc ggattgcggc gagaaaacct atgttggtag
    18721 cggtcgcctg aaagatcgta aagcactggt gacagggggc gattccggaa taggtcgcgc
    18781 tgccgccatc gcttacgcgc gtgaaggggc tgacgtggcg atcagttatc ttcccgtgga
    18841 agaagaagac gctcaggatg tgaaaaagat cattgaagaa tgcggacgca aagccgttct
    18901 gctgccaggc gatttaagcg atgagaaatt cgcccgttcg ctggttcacg aagcgcacaa
    18961 ggcgttaggc gggctggata ttatggcgct ggtcgccggg aaacaggttg ccattccgga
    19021 tattgcagac ctcaccagcg aacagtttca aaagaccttt gccattaacg ttttcgcgct
    19081 gttctggcta acccaggaag cgatccccct gctaccgaaa ggtgcaagta tcatcaccac
    19141 ttcgtcaatc caggcatacc agccaagtcc gcatttactg gactatgcgg ctacgaaggc
    19201 ggcgattctg aactacagcc gtggcctggc aaaacaggtc gcggagaaag gtattcgggt
    19261 gaatattgtc gcgccaggcc ctatctggac ggcactgcaa atttccggcg gacaaacgca
    19321 ggataagatc ccgcagtttg gtcagaaaac gccgatgaag cgtgcggggc aaccggcgga
    19381 actggcccct gtatatgttt atctggcaag tcaggagtcg agctacgtca ccgcagaagt
    19441 gcacggcgtg tgcggcggcg agcatttagg ttaaaaaaat gcccggttgt gaaaagcaac
    19501 cgggcatcat tgtgaattca ctcttacctg atgtggcgaa aacgccttat ccggcctaca
    19561 aacgcacgaa gattcaacat gttgcaatag attttgtagg cctgatgagc gtagcgcatc
    19621 aggcaattct actttacttc gctttggcgg tttcttcgcc gaccagacct atcttcaggt
    19681 aacccgcctg atgcagcgta tccattacct tcatcaacgt ctcgtaatcg acggttttgt
    19741 ccgctcggaa gaagatggtg gtgtctttct tgccttcggt taacgcattc aacgccgtaa
    19801 tcattgtttc atcggtgacc ggatcgttac cgataaacat cgagttgtct gccttcaccg
    19861 acagataaac cggtttttcc gggcgcggct gcggcgtgct ggtagaagca ggcaagttca
    19921 ccttcacatc taccgtcgct aacggtgccg ccaccataaa gataatcagc agaaccaaca
    19981 tcacgtcgat aaacggcgtc acgttgatat catgcatttc gccgttatcg tcgaggtttt
    20041 cgttaagatg cattgccatc ggacattatc ctgcgcgtaa tttttgtgcg acacgaaccg
    20101 gatgcgcagc ggcgctggct tccagatcca ggtcacggct ttgcagcaac aatacctgcg
    20161 ctgcaacatc acccagcatc gctttaaagc cgccaatctg gcgtgcaaat acgttataga
    20221 taacgaccgc aggaatcgct gccacgaggc cgattgccgt tgctaacaga gcttctgcga
    20281 tacccggcgc aacgactgcc aggttagtgg tctgcgtttg cgcgatacca ataaagctgt
    20341 tcatgatgcc ccagaccgta ccaaacagac caacgaacgg agaaatcgcg ccgatggttg
    20401 ccagatagcc gttaccgcgg cccatttgac gacccactgc ggcgacccga cgctccagac
    20461 ggaagctggt acgttcttta ataccttcgt tatcgtcgct gccttctgac agttccagct
    20521 cattctgcgc ttcattgagc aaatgcaggc ttaagctttt gctaccaaaa tcagcggcga
    20581 tatcgttggc ctggtttaag gaacgcgctt cagccagcag ttgctgctcg cgcttaaggc
    20641 gacgcttctg attgaagaac tctacgctct tactgaagaa gattgcccaa gtgactacgg
    20701 aggccaaaat aagcccaatc atcacgcact taacgacaat atcggcgtgc tgatacatac
    20761 cccagacgga aaggtccgtc tgcatcaaat tattacccac tgtgtatctc caggacgcaa
    20821 gtcacaaaat ctgcgcataa taatatcaaa atgacgtcga attgatagtc gttctcatta
    20881 ctatttgcat actgccgtac ctttgctttc ttttccttgc gtttacgcag taaaaaagtc
    20941 accagcacgc catttgcgaa aattttctgc tttatgccaa ttcttcagga tgcgcccgcg
    21001 aatattcatg ctagtttaga catccagacg tataaaaaca ggaatcccga gatggcggac
    21061 aaaaagcttg atactcaact ggtgaatgca ggacgcagca aaaaatacac tctcggcgcg
    21121 gtaaatagcg tgattcagcg cgcttcttcg ctggtctttg acagtgtgga agccaaaaag
    21181 cacgcgacgc gcaatcgcgc caatggcgag ttgttctatg gacggcgcgg aacgttaacc
    21241 catttctcct tacaacaagc gatgtgtgaa ctggaaggtg gcgcaggctg cgtgctattt
    21301 ccctgcgggg cggcagcggt tgctaattcc attcttgctt ttgtcgaaca gggcgatcat
    21361 gtgttgatga ccaacaccgc ctatgaaccg agtcaggatt tctgtagcaa aatcctcagc
    21421 aaactgggcg taacgacatc gtggtttgat ccgctgattg gtgccgatat cgttaagcat
    21481 ctgcagccaa acactaaaat cgtgtttctg gaatcgccag gctccatcac catggaagtc
    21541 cacgacgttc cggcgattgt tgccgccgta cgcagtgtgg tgccggatgc catcattatg
    21601 atcgacaaca cctgggcagc cggtgtgctg tttaaggcgc tggattttgg catcgatgtt
    21661 tctattcaag ccgccaccaa atatctggtt gggcattcag atgcgatgat tggcactgcc
    21721 gtgtgcaatg cccgttgctg ggagcagcta cgggaaaacg cctatctgat gggccagatg
    21781 gtcgatgccg ataccgccta tataaccagc cgtggcctgc gcacattagg tgtgcgtttg
    21841 cgtcaacatc atgaaagcag tctgaaagtg gctgaatggc tggcagaaca tccgcaagta
    21901 gcgcgagtta accaccctgc tctgcctggc agtaaaggcc acgaattctg gaaacgagac
    21961 tttacaggca gcagcgggct attttccttt gtgcttaaga aaaaactcag taatgaagag
    22021 ctggcgaact atctggataa cttcagttta ttcagcatgg cctactcgtg gggcgggtat
    22081 gaatcgttga tcctggcgaa tcaaccagaa catatcgccg ccattcgccc acaaggcgag
    22141 atcgatttta gcgggacctt gattcgcctg catattggtc tggaagatgt cgacgatctg
    22201 attgccgatc tggacgccgg ttttgcgcga attgtataac attgccactt ttggacaatt
    22261 ttgcagacat ttaattgtga aaagtcttaa attgttgcgt ccgggatcaa ggcgtcccgg
    22321 acgaatcagg agtacaatag gcagataaag gcttaaacgc tgttccacag gaaagtccat
    22381 ggctgttatt caagatatca tcgctgcgct ctggcaacac gactttgccg cgctggcgga
    22441 tcctcatatt gttagcgttg tttactttgt catgtttgcc acgctgtttt tagaaaacgg
    22501 cctgctgccc gcctcatttt tgccaggcga cagcttgttg atactggcag gcgcattgat
    22561 tgcccaaggg gttatggatt ttctgcctac gattgcgatt ctgaccgccg cagcaagtct
    22621 gggctgctgg ctaagttata ttcaggggcg ctggttaggg aataccaaaa cggtgaaagg
    22681 ctggctggca cagcttcctg ctaaatatca ccagcgcgcc acctgcatgt ttgaccgcca
    22741 cggtctgctg gcgctgctgg ctggacgttt tcttgcattt gtccgtacgc tgctgccaac
    22801 catggcggga atttccggtc tgccaaaccg ccgcttccag tttttcaact ggttaagtgg
    22861 attgctgtgg gtcagcgtgg taaccagttt tggctatgcc ttaagtatga ttccgttcgt
    22921 taaacgccat gaagatcagg taatgacgtt cctgatgatc ctgccaattg ccttgttaac
    22981 cgctggcttg ttaggcacgc tgtttgtagt gattaaaaaa aaatactgta acgcctgacg
    23041 attttccccg ttcccggctg ctgtaccggg aacgtattta attcccctgc atcgcccgca
    23101 ttcttgccgc atcttccccc ggcgtcacac cgaagtaacg tttaaactca cggctaaatt
    23161 gcgatgcgct ttcatagtcg acgcgcatcg ctgctgcgct ggccttcatg ccgtcatgga
    23221 tgatcatcat ccgcgcctta tgcagacggt aattcttcaa atactgcaac ggcgaggtgc
    23281 tggtgacaga cttaaaatta tgatggaacg ccgatacgct catgttggct tctgccgcca
    23341 gttgctcgac gctcaggttt tcggtgtatt tattctcaat ccgtttcagc acgcggctaa
    23401 tcagactgaa gtgagtctgg cgactgacca gcgccagtaa cgcgccgccg caaggtccgg
    23461 tcagcacgta gtacagaatt tcgcggatga tctgtttgcc gagaatacgc gcatccagtg
    23521 gtcgctccat cacgtcgagt aaccgctccg ccgcgcataa aatctcttct gataacgtgg
    23581 cggagttaat cccgctggct gccatcgacg gctggaaatg ctcatcttcg ccaatgtcca
    23641 tcaacagttc ctgtaactgc aaaatatcga cattgagacg caatcctgcc agcggcacct
    23701 ctgacgtggc ataggtttcg cactcaaacg gcaacggcac cgtcagcagc aggtattcat
    23761 tggcatcata acgaaacacg cgttcattga tataaccgat tttatgcccg gaaaagagaa
    23821 ttatgatgcc aggctcgtac atcaccggtg tacgtgcgaa aggcgtctcg ccatacaaca
    23881 aacgcacatc gggcaacagt tctgacaaac tattttcttt atttttcagt ttattaactt
    23941 tatccgccag caagcggcaa atctcttcac gtttcatatc gcgtaatttc ttaggaataa
    24001 tgcggcaatt tgattgtgcg caattttgta gcatttctcc agcactctgg agaaataggc
    24061 aagacattgg cagaaatgag cattgagagc caaggcgctg gcgatcacaa tgaaaaacat
    24121 caggcagatc gttctctgcc ctcatattgg cccagcaaag ggagcaagta atgaacaact
    24181 ttaatctgca caccccaacc cgcattctgt ttggtaaagg cgcaatcgct ggtttacgcg
    24241 aacaaattcc tcacgatgct cgcgtattga ttacctacgg cggcggcagc gtgaaaaaaa
    24301 tcggcgttct cgatcaagtt ctggatgccc tgaaaggcat ggacgtgctg gaatttggcg
    24361 gtattgagcc aaacccggct tatgaaacgc tgatgaacgc cgtgaaactg gttcgcgaac
    24421 agaaagtgac tttcctgctg gcggttggcg gcggttctgt actggacggc accaaattta
    24481 tcgccgcagc ggctaactat ccggaaaata tcgatccgtg gcacattctg caaacgggcg
    24541 gtaaagagat taaaagcgcc atcccgatgg gctgtgtgct gacgctgcca gcaaccggtt
    24601 cagaatccaa cgcaggcgcg gtgatctccc gtaaaaccac aggcgacaag caggcgttcc
    24661 attctgccca tgttcagccg gtatttgccg tgctcgatcc ggtttatacc tacaccctgc
    24721 cgccgcgtca ggtggctaac ggcgtagtgg acgcctttgt acacaccgtg gaacagtatg
    24781 ttaccaaacc ggttgatgcc aaaattcagg accgtttcgc agaaggcatt ttgctgacgc
    24841 taatcgaaga tggtccgaaa gccctgaaag agccagaaaa ctacgatgtg cgcgccaacg
    24901 tcatgtgggc ggcgactcag gcgctgaacg gtttgattgg cgctggcgta ccgcaggact
    24961 gggcaacgca tatgctgggc cacgaactga ctgcgatgca cggtctggat cacgcgcaaa
    25021 cactggctat cgtcctgcct gcactgtgga atgaaaaacg cgagaccaag cgcgctaagc
    25081 tgctgcaata tgctgaacgc gtctggaaca tcactgaagg ttccgacgat gagcgtattg
    25141 acgccgcgat tgccgcaacc cgcaatttct ttgagcaatt aggcgtgccg acccacctct
    25201 ccgactacgg tctggacggc agctccatcc cggctttgct gaaaaaactg gaagagcacg
    25261 gcatgaccca actgggcgaa aatcatgaca ttacgttgga tgtcagccgc cgtatatacg
    25321 aagccgcccg ctaagct
//
LOCUS       DAKISA010000057        25287 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.057, whole genome shotgun sequence.
ACCESSION   DAKISA010000057 DAKISA010000000
VERSION     DAKISA010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25287
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.057"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..573)
                     /locus_tag="OVW45_002441"
     CDS             complement(<1..573)
                     /locus_tag="OVW45_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HCV5526865.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGI
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEW"
     gene            789..2222
                     /gene="cscA"
                     /locus_tag="OVW45_002442"
     CDS             789..2222
                     /gene="cscA"
                     /locus_tag="OVW45_002442"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCV5526866.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDCAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(2230..3288)
                     /locus_tag="OVW45_002443"
     CDS             complement(2230..3288)
                     /locus_tag="OVW45_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCV5526867.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            3456..3674
                     /locus_tag="OVW45_002444"
                     /pseudo
     CDS             3456..3674
                     /locus_tag="OVW45_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3692..5020
                     /gene="dsdA"
                     /locus_tag="OVW45_002445"
     CDS             3692..5020
                     /gene="dsdA"
                     /locus_tag="OVW45_002445"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCV5526868.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(5128..6666)
                     /gene="emrY"
                     /locus_tag="OVW45_002446"
     CDS             complement(5128..6666)
                     /gene="emrY"
                     /locus_tag="OVW45_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCV5526869.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6666..7829)
                     /gene="emrK"
                     /locus_tag="OVW45_002447"
     CDS             complement(6666..7829)
                     /gene="emrK"
                     /locus_tag="OVW45_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCV5526870.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            8245..8859
                     /gene="evgA"
                     /locus_tag="OVW45_002448"
     CDS             8245..8859
                     /gene="evgA"
                     /locus_tag="OVW45_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCV5526871.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGILVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8864..12457
                     /gene="evgS"
                     /locus_tag="OVW45_002449"
     CDS             8864..12457
                     /gene="evgS"
                     /locus_tag="OVW45_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCV5526872.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVILDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRTLNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYSTGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALICSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(12513..13658)
                     /gene="yfdE"
                     /locus_tag="OVW45_002450"
     CDS             complement(12513..13658)
                     /gene="yfdE"
                     /locus_tag="OVW45_002450"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCV5526873.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13732..14676)
                     /gene="yfdV"
                     /locus_tag="OVW45_002451"
     CDS             complement(13732..14676)
                     /gene="yfdV"
                     /locus_tag="OVW45_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCV5526874.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14746..16440)
                     /gene="oxc"
                     /locus_tag="OVW45_002452"
     CDS             complement(14746..16440)
                     /gene="oxc"
                     /locus_tag="OVW45_002452"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCV5526875.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(16494..17744)
                     /gene="frc"
                     /locus_tag="OVW45_002453"
     CDS             complement(16494..17744)
                     /gene="frc"
                     /locus_tag="OVW45_002453"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCV5526876.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDSAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(18257..18880)
                     /locus_tag="OVW45_002454"
     CDS             complement(18257..18880)
                     /locus_tag="OVW45_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCV5526877.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            19177..19452
                     /gene="ypdI"
                     /locus_tag="OVW45_002455"
     CDS             19177..19452
                     /gene="ypdI"
                     /locus_tag="OVW45_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HCV5526878.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(19529..19771)
                     /locus_tag="OVW45_002456"
     CDS             complement(19529..19771)
                     /locus_tag="OVW45_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCV5526879.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            20124..21044
                     /gene="lpxP"
                     /locus_tag="OVW45_002457"
     CDS             20124..21044
                     /gene="lpxP"
                     /locus_tag="OVW45_002457"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCV5526880.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNPKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            21400..21471
                     /gene="ypdK"
                     /locus_tag="OVW45_002458"
     CDS             21400..21471
                     /gene="ypdK"
                     /locus_tag="OVW45_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCV5526881.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(21536..22774)
                     /gene="alaC"
                     /locus_tag="OVW45_002459"
     CDS             complement(21536..22774)
                     /gene="alaC"
                     /locus_tag="OVW45_002459"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCV5526882.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDITEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            23151..24848
                     /gene="ypdA"
                     /locus_tag="OVW45_002460"
     CDS             23151..24848
                     /gene="ypdA"
                     /locus_tag="OVW45_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HCV5526883.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSDGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            24863..>25287
                     /gene="ypdB"
                     /locus_tag="OVW45_002461"
     CDS             24863..>25287
                     /gene="ypdB"
                     /locus_tag="OVW45_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCV5526884.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLV"
BASE COUNT         7049 a         5968 c         5583 g         6687 t
ORIGIN      
        1 ccattcttct tccgagagct tgacgacatc cgccagttgt agcgcctgcc gcaaacacaa
       61 gcggagcaaa tgctcgtctt gccatagatc ttcacgaata ttaggatcga agctgacaaa
      121 acctccggca tgccggatcg ccgtcatcgc agtaaatgcg ctggtacgcg aaggctcggc
      181 agacaacgca attgaacaga gatgtaacca ttcgccatgt cgccagcagg gcaagtctgt
      241 cgtctctaaa aaaagatcgg cactggggcg gaccataaac gtaaatgaac gttccccttg
      301 atcgttcaga tcgacaagca ccgtggatgt ccggtgccat tcatcttgct tcagatacgt
      361 gatatcgact ccctcagtta gcagcgttct ttgcattaac gccccaaaag gatcatcacc
      421 gacccgacct ataaatccac ttattccgcc taatctggcg attcccaccg caacgttagc
      481 tggcgcgccg ccaggacaag gcagtaggcg cccgtctgat tctggcaaga gatctacgac
      541 cgcatcccct aaaacccata ctttggctga catttttttc ccttaaattc atctgagtta
      601 cgcatagtga taaacctctt tctcgcaaaa tcgtcatgga tttactaaaa catgcatatt
      661 cgatcacaaa acgtcatagt taacgttaac atttgtgata ttcatcgcat ttatgaaagt
      721 aagggactat atttttataa aagttaacgt taacaattca ccaaatttgc ttaaccagga
      781 tgattaaaat gacgcaatct cgattgcatg cggcgcaaaa cgcactagca aaacttcatg
      841 agcaccgggg taacactttc tatccccatt ttcacctcgc gccacctgcc ggatggatga
      901 acgatccaaa cggcctgatc tggtttaacg atcgttatca cgcgttttat caacatcacc
      961 cgatgagcga acactgggga ccaatgcact ggggacatgc caccagcgac gatatgatcc
     1021 actggcagca tgagcctatt gcgctagcgc caggagacga taatgacaaa gacgggtgtt
     1081 tttcaggtag tgctgtcgat gacaatggtg tcctctcact tatctacacc ggacacgtct
     1141 ggctcgattg tgcaggtaat gacgatgcaa ttcgcgaagt acaatgtctg gctaccagtc
     1201 gggatggtat tcatttcgag aaacagggtg tgatcctcac tccaccagaa ggaatcatgc
     1261 acttccgcga tcctaaagtg tggcgtgaag ccgacacatg gtggatggta gtcggggcga
     1321 aagatccagg caacacgggg cagatcctgc tttatcgcgg cagttcattg cgtgaatgga
     1381 ccttcgatcg cgtactggcc cacgctgatg cgggtgaaag ctatatgtgg gaatgtccgg
     1441 actttttcag ccttggcgat cagcattatc tgatgttttc cccgcaggga atgaatgccg
     1501 agggatacag ttaccgaaat cgctttcaaa gtggcgtaat acccggaatg tggtcgccag
     1561 gacgactttt tgcacaatcc gggcatttta ctgaacttga taacgggcat gacttttatg
     1621 caccacaaag ctttttagcg aaggatggtc ggcgtattgt tatcggctgg atggatatgt
     1681 gggaatcgcc aatgccctca aaacgtgaag gatgggcagg ctgcatgacg ctggcgcgcg
     1741 agctatcaga gagcaatggc aaacttctac aacgcccggt tcacgaagct gagtcgttac
     1801 gccagcagca tcaatctgtc tctccccgca caatcagcaa taaatatgtt ttgcaggaaa
     1861 acgcgcaagc agttgagatt cagttgcagt gggcgctgaa gaacagtgat gccgaacatt
     1921 acggattaca gctcggcact ggaatgcggc tgtatattga taaccaatct gagcgacttg
     1981 ttttgtggcg gtattaccca cacgagaatt tagacggcta ccgtagtatt cccctcccgc
     2041 agcgtgacac gctcgcccta aggatattta tcgatacatc atccgtggaa gtatttatta
     2101 acgacgggga agcggtgatg agtagtcgaa tctatccgca gccagaagaa cgggaactgt
     2161 cgctttatgc ctcccacgga gtggctgtgc tgcaacatgg agcactctgg ctactgggtt
     2221 aacataatat caggtggaac aacggatcaa cagcggacaa gggatccgcg tcactcttcc
     2281 cccttcacga ccttcaataa tatgcaatgc agcttcccgc ccgataatgt catgtggaag
     2341 ctgaattgtg gtcagcggcg gtaaaaacag atgcccgacg ccaaccagat tatcaaagcc
     2401 cattacggcg acatcctgcg ggatacgtac ccccttcgcc aggagaacct gataagcgac
     2461 aaaggctgca cgatcgttac cacatatcag aacatcaaaa tctggtttgc ccgatttgaa
     2521 gtgggcattg agtaaacttg cgagatcggt gtagtgatca tcacctgttg ccatgtgaaa
     2581 ttgtttcacc tcagccagat ctcgtccagc atcacgccag gcctgctcaa atccctgccg
     2641 acgataccct gttgccaacg cactttccgg tagccagaag cataacggtt gacgatagcc
     2701 cgccgcgagc aaatgctgtg ttgattcata ttgtgcagtg taatcatcag ggatataact
     2761 gggtaacgct gggtcatccg ccacacagtt cgccaataca atattttcac catacagaga
     2821 ctcaggcagc gtgatatgtc gcagccccat tgtagtatag ataatgccat ccggacggtg
     2881 ggcaagcagc tgacgtgccg cgcgggcagc gtcatcttca gaaaaaatat tgattaaaaa
     2941 actattccag ccgaactcgc tggcggtttg ctcaatggca agcagaatat caacagagaa
     3001 aggagtggta gccgtgtcct gcgccagcac ggcgagagtc gacggcttac gtccttgagc
     3061 gcgcatctta cgggcggaaa gatcaggaac ataattcagg gtctggattg cctgcaatac
     3121 gcggtcacgc gttgcaggac gcacagattc tgcattatgc atcacccggg agactgtcat
     3181 catcgacact cccgccaggc gtgcgacatc ctttaatgaa gccataccca agccgtttgc
     3241 cgtaaaacgg gcactgtagc agaaacagac gtcactggcg agatccaacg ccctatcacc
     3301 tgacacagca atacaataaa aaataacaat aattcccgga caattgtccc caattccgcc
     3361 tctgttctcg ccaacgagta accatatagg caagtaagta aaacatgcca actggcatcg
     3421 ccaacctgga gaagaaaata taaatatgga tcattgtgct gccgctgtat cccgacatca
     3481 gcccggaaat tattgcgatt gctatcggtt caggtgcaat tggctgcacg atcgttacgg
     3541 actcgctttt ctggctagtg aagcaatatt gcggcgctac gctcaatgaa acatttaaat
     3601 actatacgac agcgacattt atcgcttcag tcgtcgctct ggcgggcaca ttcctgctgt
     3661 catttatcat ctaagcgcaa agagacgtac tatggaaaac gctaaaatga actcgctcat
     3721 cgcccagtat ccgttggtaa aggatctggt tgctcttaaa gaaaccacct ggtttaatcc
     3781 tggcacgacc tcattggctg aaggtttacc ttatgttggc ctgaccgaac aggatgttca
     3841 ggacgcccat gcgcgcttat cccgttttgc accctatctg gcaaaagcat ttgctgaaac
     3901 tgctgccact ggggggatta ttgaatcaga actggttgcc attccagcta tgcaaaaacg
     3961 gctggaaaaa gaatatcagc aaccgatcag cgggcaactg ttactgaaaa aagatagcca
     4021 tttgcccatt tccggctcca taaaagcacg cggcgggatt tatgaagtcc tggcacacgc
     4081 agaaaaactg gctctggaag cggggttgct gacgcttgat gatgactaca gcaaactgct
     4141 ttctccggag tttaaacagt tctttagcca atacagcatt gctgtgggct caaccggaaa
     4201 tctggggtta tcaatcggca ttatgagcgc ccgcattggc tttaaggtga cagttcatat
     4261 gtctgctgat gcccgggcat ggaaaaaagc gaaactgcgc agccatggcg ttacggtcgt
     4321 ggaatatgag caagattatg gtgttgccgt cgaggaagga cgtaaagcag cgcagtctga
     4381 cccgaactgt ttctttattg atgacgaaaa ttcccgcacg ttgttccttg ggtattccgt
     4441 cgctggccag cgtcttaaag cgcaatttgc ccagcaaggc cgtatcgtcg atgctgataa
     4501 ccctctgttt gtctatctgc cgtgtggtgt tggcggtggt cctggtggcg tcgcattcgg
     4561 gcttaaactg gcgtttggcg atcatgttca ctgctttttt gccgaaccaa cgcactcccc
     4621 ttgtatgttg ttaggcgtcc atacaggatt acacgatcag atttctgttc aggatattgg
     4681 tatcgacaac cttaccgcag cggatggcct tgcagttggt cgcgcatcag gctttgtcgg
     4741 gcgggcaatg gagcgtctgc tggatggctt ctataccctt agcgatcaaa ccatgtatga
     4801 catgcttggc tggctggcgc aggaagaagg tattcgtctt gaaccttcgg cactggcggg
     4861 tatggccgga cctcagcgcg tatgtgcatc agtaagttac caacagatgc acggtttcag
     4921 cgcagaacaa ctgcgtaata ccactcatct ggtgtgggcg acgggaggtg gaatggtgcc
     4981 ggaagaagag atgaatcaat atctggcaaa aggccgttaa taacgtttca acgcagcatc
     5041 gcaatccttt ccctgggtga gcgatgctgc cgatggcgca gacttaagat ccccggtctt
     5101 acccgctata accccctcct tttatcatca cccaacgcct ttcgctgtaa acggcggttt
     5161 cgcaaaccaa accaaaaccg taagtaagat aaaagcaata gccgccatac ggaaaatctc
     5221 atttgcagaa atagaaagtg actgctgggt tatttcatta ttaatttcat taagaactcc
     5281 tgaaagcgaa ccataatatt tatccataat ttgtgacgat gaattaaaca cggggttaaa
     5341 ttgatcgatg gttgctgtca actgactatg gtgtaacgat tcgcgtcgtc cccacagcgt
     5401 cattgtcaac gacgtaccaa ctgatcctga caaggtacga aaaaaattac tcatactcga
     5461 ggcattggca aatttattat ctggcaagcc tgaaaacgaa atcgttgtta agggtaaaaa
     5521 gaaacaggca acggcgaatc cctgaaaaaa ctgcggcaaa atgatgcctg taaaatcaat
     5581 cgttggcata aatgtcacag aacgccagta atagcaaacc gcatacatca aaaaactaaa
     5641 tgtcactaac aaccgcatgt ctattttgtt gccataacgt cctatcaaag gtgaaattaa
     5701 tagtggcatg atgccgatgg gcgcataagc aagtccggcc catatcgcat tataccccat
     5761 cgtttcctgg agtaactgcg gcataaggac gatcgctcca gagtaaaata aatacgcgca
     5821 tgtgatactc acaataccaa tggtgaagtt acgggactta aacaaactga gatcaagaat
     5881 cgggttctct gaggtcgact cccaaatgac taaagagatc agagaaataa ctgatactac
     5941 tgttaatatt attattgtac tcgagttgaa ccaatccaga tcgcgccctt tatcaagcat
     6001 aatttgcaag ccaccaacac cgagcactaa cagggtcagt cctggtagat tcattttgac
     6061 cggtgaagtc tcagtttctc ttcctttaag taaggttaag cataatgtca ggacgataat
     6121 ccccataggg acattgatta aaaatatcca accccagcta aagttatcac aaatataacc
     6181 gcccaatatc ggcccacata tcggagcgat aatcacggtc attgaccata atgccagagc
     6241 aaatgttctt ttttctggcg gataattcct taataataaa ctctgtgaca gtggaattaa
     6301 cggccccgcc attaacccct gaacgactct aaaaaatatc agcacatcaa gattggtcga
     6361 taggctacac attaatgaag acagcgaaaa aaaggttact gaaagtaaaa ataatcttaa
     6421 ttcgcctatt ctttgtgcca acctgccagt aacagggatc gcaatggcat ttgctacacc
     6481 aaacgaggtg ataacccagg tgccttcgtc tgttgatgct cccagaaagc cagatattgt
     6541 cggtattgcg acgttagaaa tagtggaatc caacatttgc ataaatgtcg ctaatgacaa
     6601 tgcaatagtg acgcaccata acgtcccacc ggttaatggt gccggagttg atttagtgat
     6661 tgccattaaa gttgtccatt atgcgaaata atgttgctaa tttctttttc tatcggactg
     6721 gtatcgataa ctaaagcctt actggtataa gccggcatgg aggtcacggt tgaagccagc
     6781 tcaggcatct cagcaatgtc ttcgtccttc gtatcaatag ttgcagtcat cgataaacca
     6841 atgcgcaagg ggtgttccat gagttctttt ggatcaagag aaacttcaac cggtacacgc
     6901 tgaacgattt tgatccagtt ccctgtcgca ttttgtgcag gtaataagga gaacgcattg
     6961 ccggttccca tattgatccc tgtcacccga ccatgaaaca caacattttc accataaaga
     7021 tcgctgataa tattgactga ttgaccaatc cgtacatccg tgagttgtgt ttctttaaag
     7081 ttggcattaa cccacatttg acgtgccggt acgacagcca ttaacgattg tccgggactc
     7141 actgtttcgc cgacctgaac acttcgctgg gcaatatagc cagtaaccgg actccttata
     7201 tccgtacgtt taagcgccaa ccaggcttct ttggttgcat ccgccgcttc aactacttgt
     7261 ggctgacggt ttaatggtgt gttcattact aaagctttat tcgctttata agcctggata
     7321 gcggcattca gtgccgcttt gctacttatt aacgtatctt tggtatgctc cagcgtttct
     7381 tttgaaataa ccccctgctt cgctaaaggc acacgacggt tataatcttc taaggattgt
     7441 tgatactgaa tacgtgctga agcgacttcg gcactgtatt gtttatcctg taagtacagt
     7501 ttattcgttt gccgaacaat atttgccaga ttatttttag ctttattgag tgcgatagtg
     7561 gcatcagttt tatccagtga aactaaaatg tcaccctgtc gaacgtagtt cgtatcttta
     7621 tgattaacta cagtgacact acctgagact tgtgcagaaa ttggatctgc attccccgtg
     7681 acataggcgt catctgtact aatcatgtct tctaattcca ttgaccaata ggcataggca
     7741 cctgaaaacg caataaataa aactatcacc aataaagaaa agtatttttt tctgttagac
     7801 tgttttttat ttgaattaat ctgttccact attatctctc atttcgcata gatgaaattt
     7861 atgaattgaa tgaaaagagt attcattctt catatcaaac acagtgcatg tgttgcacat
     7921 tatcccagga acgttggagg cctaatttaa atgaggtgca aaaaaaacaa cagaggacta
     7981 aaccgtggct tttgcaatac aattcttacg cctgtaggat tagtgagaag acttatagtg
     8041 ctaacttgaa actataaatc atcggtataa tccctgattt tattgttgac atttcattta
     8101 tgccgactat ttatatggta tacttgtcga attatcttaa aggaagctca gattttctta
     8161 tttttattga gaaaatgaga tgacgcctta tgtctgtatt actacaggga gaagggagat
     8221 gcttcattgc aaagggaata atctatgaac gcaataatta ttgatgacca tcctcttgct
     8281 atcgcagcaa ttcgtaattt attgatcaaa aacgatattg aaatcttagc ggagttaact
     8341 gaaggcggaa gcgccgttca gcgggtggaa acacttaagc ctgatatcgt catcattgat
     8401 gtcgatatcc ccggagttaa cggtatcctg gtgttagaaa cgctgaggaa gcgccaatat
     8461 agcggaatta ttattatcgt ctccgctaaa aatgaccatt tttacgggaa acattgtgct
     8521 gatgctggcg ctaatggttt cgtgagtaaa aaagaaggca tgaacaatat cattgcggct
     8581 attgaagctg caaaaaatgg ctactgctat ttccccttct ctctcaaccg gtttgttgga
     8641 agtttaacgt ccgaccagca aaaactcgac tccttatcga aacaagaaat tagtgtcatg
     8701 cggtatattc ttgatggcaa ggataataat gacattgcgg aaaaaatgtt catcagcaac
     8761 aaaactgtca gcacttataa aagtcgcctg atggaaaaat tagaatgtaa atcactgatg
     8821 gatctttaca cattcgcaca acgtaacaaa atcggctaac cacatgaagt ttttacccta
     8881 tatttttctt ctctgttgtg gtctttggtc gaccataagt ttcgcagacg aagattacat
     8941 cgaatatcgt ggcatcagta gtaacaaccg tgtcatactt gatccagtac gtctgagcaa
     9001 caaggaatta cgttggttag cgagcaaaaa aaatcttgtg attgcagtac ataagtccca
     9061 aacggctacg ttgttgcata ccgattcgca gcaacgagtt cgtggtatta atgctgatta
     9121 tttaaatctt ttaaaaagaa cgttaaatat caaattaaca ctccgggaat acgccgatca
     9181 tcaaaaagca atggacgcgc tggaagaagg tgaagtcgat atagtgttat cacatttagt
     9241 agcttcgccg cctcttaatg atgacattgc tgcaaccaaa ccactgataa ttacctttcc
     9301 ggcgctggta accacccttc acgattcaat gcgaccgctt acctcatcaa aaccagtaaa
     9361 tattgctcga gtagcaaatt atccccctga cgaggtaatt catcaatcat ttccaaaagc
     9421 aacaattatc tcttttacaa atttatatca ggcattagca tccgtctcag ccggacagaa
     9481 tgattacttt attggtagta acatcattac cagcagtatg atttcccgct atttcactca
     9541 ctccttaaat gtagtgaaat attataactc accgcgtcaa tataattttt tcttgaccag
     9601 aaaagaatct gtcgttctta atgaagtact caatcgattt gttgatgctt tgacaaatga
     9661 agttcgctat gaagtatcac aaaattggct tgatacagga aacctggcct ttctgaacaa
     9721 accattagaa ctcactgaac atgaaaaaca atggattaag cagcatcccg atttaaaggt
     9781 gctggaaaat ccttactccc ctccctattc tatgacggat gaaaatggct cggttcgggg
     9841 cgttatgggg gatattctta atattattac tttgcaaaca ggtttaaatt tttctccgat
     9901 caccgtttca cacaatatcc atgctggaac acagctgaat cccggcggat gggatatatt
     9961 acccggtgct atttatagtg aagatagaga aaataatgtt ttatttgctg aagccttcat
    10021 aacaacgcct tacgtttttg tcatgcaaaa agcgcctgac agtgaacaaa cattaaaaaa
    10081 aggaatgaaa gttgccattc catattatta tgagcttcat tcgcaattaa aagagatgta
    10141 tccggaggta gagtggataa aagtcgataa cgccagcgct gcatttcaca aggtcaagga
    10201 aggcgaactt gatgctctgg tcgcgacaca gttaaattca cgttacatga tcgaccatta
    10261 ctatcctaat gaactttatc attttcttat tcccggcgtt ccgaatgcat cgctttcgtt
    10321 cgcttttcct cgcggagaac cggaacttaa ggatattatt aataaagcac tgaatgcaat
    10381 ccccccaagc gaagttctgc gcctgaccga aaaatggatt aaaatgccca atgtgaccat
    10441 tgacacatgg gacctctata gcgagcaatt ttatattgtt acgacattat ccgttttatt
    10501 agttggcagt agccttttat ggggattcta cctgttacgc tcagttcgtc gtcgtaaagt
    10561 cattcagggt gatttagaaa accaaatatc attccgaaaa gcactctcgg attccttacc
    10621 gaatccaact tatgttgtaa actggcaagg taatgtcatt agtcataata gtgcttttga
    10681 acattatttc actgcggatt actacaaaaa tgcaatgtta ccattagaaa acagtgactc
    10741 cccctttaaa gatgtttttt ctaatgcgca tgaagtcaca gcagaaacga aagaaaatcg
    10801 aacaatatac acacaggttt ttgaaattga taatggcatc gagaaaagat gcattaatca
    10861 ctggcataca ttatgcaatc tgccagccag cgatcatgct gtttatattt gtggttggca
    10921 agatattaca gagacgcgtg atttaattca tgcactcgaa gtagagagaa ataaagcgat
    10981 caatgcaact gtcgcaaaaa gtcagtttct ggcaacaatg agtcacgaaa taagaacacc
    11041 aataagctcc attatgggct tcctggaact tctgtcgggt tctggtctta gcaaggagca
    11101 acgggtggag gcgatttcac ttgcctactc caccggacaa tcactcctcg gcttaattgg
    11161 tgaaatcctt gatgtcgaca aaattgaatc gggtaactat caacttcaac cacaatgggt
    11221 cgatatccct actttagtcc agaacacttg tcactctttc ggtgcgattg ctgcaagcaa
    11281 atcgatcgca ttaatttgca gcagtacatt tcctgatcat tacctggtta agatcgaccc
    11341 tcaggcgttt aagcaggtct tatcaaattt actgagcaat gctctcaaat ttaccaccga
    11401 gggggcagta aaaattacga cctccctggt tcacattgat gacaaccacg ctgttatcaa
    11461 aatgacgatt atggattctg gaagtggatt atcgcaggaa gaacaacaac aactctttaa
    11521 acgctacagc caaacaagtg caggtcgcca gcaaacagga tctggcttag gcttaatgat
    11581 ctgcaaagaa ttaattaaaa atatgcaggg cgatttgtca ttagaaagtc atccaggcat
    11641 aggaacaaca tttacgatca caatcccggt agaaattagc cagcaagtgg cgactgtcga
    11701 ggcaaaagca gaacacccca tcacactacc tgaaaagttg agcatattaa tcgcggatga
    11761 tcatccgacc aacaggctat tactcaaacg ccagctaaat ctattaggat atgatgttga
    11821 tgaagccact gatggtgtgc aagcgctaca caaagtcagt atgcaacatt atgatctgct
    11881 tattactgac gttaatatgc cgaatatgga tggttttgag ttgactcgca aactccgtga
    11941 gcaaaattct tccttaccca tctgggggct tacagccaac gcacaggcta acgaacgtga
    12001 aaaagggtta agttgcggca tgaacttatg tttgttcaaa ccgttgaccc tggatgtact
    12061 gaaaacacat ttaagtcagt tacaccaggt tgcgcatatt gcacctcagt atcgccacct
    12121 tgatatcgaa gccctgaaaa ataatacggc gaacgatcta caactgatgc aggagattct
    12181 catgactttc cagcatgaaa cgcataaaga tctacccgct gcgtttcaag cactagaagc
    12241 tggcgataac agaactttcc atcagtgtat tcatcgcatc cacggtgcgg ctaacatcct
    12301 gaatttgcaa aagttgatta atattagcca tcagttagaa ataacacctg tttcagatga
    12361 cagtaagcct gaaattcttc agttgctgaa ctctgtaaaa gaacacattg cagagctgga
    12421 ccaggagatt gctgttttct gtcagaaaaa tgactaaata gcggctccca caatgttcta
    12481 atgtgggagc tatttaccag cacatcttac atttatgatg agaactcctg gcgaatttgt
    12541 tccccatgct ggtcgagcgt tgccgctccc ggcataacat gcgggtccgc gcagccgctg
    12601 atttttatcg gattacccgg catcattatt cctccggctt caatcaacat atttctcgcc
    12661 tgagtttgtg gcaaattaat ggcctcagcc acacttaata acggcgcgac gggcacacca
    12721 acttcatgta ttttggctaa ccaaacttca gctgcctgcg tttttaacgt tcgctcaata
    12781 tattgtttaa gaatagcctg gttttgtacg cgtaaaatat tgctgctaaa tcggggatca
    12841 ttaaccagtt ccgtaagctc cagtgcctgg cataacgcag aaaaaagctt gtcattacca
    12901 caacaaatcg taatcggctt atcctgagta tcgaaaacat caaaaggtgc catgtaggga
    12961 tggcgatttc ccaggcgttg tggtgacttc cctgtcgcaa tatatgccat cagaccatgc
    13021 tccagaaaac tcagcgtggc atcaaacatc gctatatcga catgcgcccc tctctggctc
    13081 ttttcgcggc cataaagtgc actcactatt ccgctgaata aatagacacc gccgcataga
    13141 tccgcaagag aggtaccaac acgcactggc ggagcatcag ggtaccctgt ttccatcatt
    13201 atcccactca ttgcctgaat gatggtatcg taggcaggag catcttttag cggaccggta
    13261 tgtccgaaac ctgacgatga agcatatatg aggcgcgggt tgatttcttg taacctttcc
    13321 catgaaaacc ccagtttttc cattgtacct gggcgaaaat tctcagctaa tacatcagct
    13381 tgtttgagca tatttataaa tatactttta tcgtgatcat tctttaaatc aagaaccaca
    13441 ctctctttgc catgattaat aaaactgtaa tagagtgact gtccatccac atagggacca
    13501 aatgtgcggg tatcatcacc atgacctggt ggctcaactt taattaccct tgcgcccata
    13561 ttacaaagaa gttgagttcc gaaaggtcca ttaaggacat gtgtcatatc gataactaat
    13621 aagccttcaa acggcccttt gctttcatta tttgtcattt gcttatgcct tgaaaaaagc
    13681 cgaaggtgaa aagacacttc ggcatttatg taaaatcaat aacatgatta attatgaaac
    13741 cagtcgactg acataaatcc acaagggagc cgtgacgaca aagcccagta cgctcacagc
    13801 caatgacgct gtaccagtac gggtgtagac attaaaccgg ctggcaataa tgattccgga
    13861 gaatgccggc ggtaatgcgc ctgccagtac catcatctgc aaatgttcgc tgttcaaatg
    13921 acatgccata ccaacgagaa gcagcgccag tggcatcaga atcaacttca ggaaggtgtt
    13981 ataagcaatt tcggcactga actcgaattt atgtgcagcc agagtcaacc ccgcagcgaa
    14041 taccgctacc cctgagttag ccttcgcaat cagattaaag gtcgggtccc atgctgccgg
    14101 aatttttacc ccaaccagca ccaggatcgt tgccagaaca ggtgcccata ctactggctc
    14161 ctttgccgca gaaattaatg cgctcagatt actattcttc ttaccatccg ctcctgagga
    14221 aggattcagc aaatacagac caataggaat agtaattgcg ttaacaataa tagaaataat
    14281 tgctaccact aaacctgttg atacggaatc accataaata ggatcgagaa ctgcaaaccc
    14341 caggaatcca atggtaggtg aacccgcaat taatgcacat acagcggctt ctgcatgggt
    14401 acgtttaaaa aatttgtagc aaccaaacca ggagaaaaag aaacatccga caataaccac
    14461 aagtgataca agggtcagac gagtgtccgc aaaaatcatt tccctgtttg cccgggtaat
    14521 agaaacaaat agagccgcag gaagagcgta gtttaatacc agtttattaa atgcgcgggc
    14581 ttgatcttct gaaaaagttt ctcgtctgcc gctaaaataa cccaacagca tgattacgat
    14641 aataggcaat aaatcgccaa taaaaaatgt tagcataata gtatttccga taagggtccc
    14701 ctcaccatcg cataatgagt tggcaagagg agacgatgtc agtatttaat taccagcgac
    14761 ttgttttggg ttaagtttgg taatatggcc actttcagtt cctgcagcag ggtcgatgac
    14821 cacattaata atggtcggtt tgcgcgactg aataccggtg gttaaagcat gacgaagttc
    14881 atctgtcgtg gtgacgttat agccaacgcc acgaaacgca tccattaatt tgtcatacct
    14941 tgcatggtgc aacagatccg ttggtgatgg tgcaccagcg ccactgagat caacaccgtc
    15001 tcctctgtag atgccgccat tattaaaaat aacgatcgtc accggcaggt tatatcgaca
    15061 aatcgtttca atttccatcc cactgaaacc aaaagcacta tcaccttcaa tggcgacaac
    15121 cggagaacca gaagtcacgc tagcaccgat ggcatagccc ataccgatgc ccatgacacc
    15181 ccaggtacca caatccagac gacgacgtgg tttatacata tcaataatat ttcgtgcatt
    15241 atccagggta tttgcacctt cattaactaa ataaatatcc tggttctcgc gcaatacgtc
    15301 gcgcacagca cttaatgcat taaagtaatt taatggttga gtatctgtac ttaatttttc
    15361 atgcattttt tgtgcatttt gctgcttgtg gatatttaaa atatcgcgcc ataccagtgg
    15421 agtcgtaaat gtgttttgtt tcagttctgc cagcatacct tgcatactgg atgcaatatc
    15481 accaacgact ggcacagcaa tggggcggtt gctgtcaatt tcctgcggtt caatatccag
    15541 ttgaataaac tgtgtatctg ccgcccatcc ttttttaccg tgtgccaata accaattcag
    15601 tcgtgcacca acaagcatga caacgtcagc atttgccagg gcaaacgaac gcgcagctgc
    15661 cgcagaaagt ggatgcgtat cttcaaggat ccctttcgcc atagacattg gcaggaatgg
    15721 aatctgagca ctttcaataa attcacgaag ctgttcatca gcttgtgaat acgccgcgcc
    15781 tttgccaagg ataattaatg gccgttcagc ttttgctaaa agcgaaattg cgctagtgac
    15841 tgacttcggg catggcaata atgctggcga cggattttca actttaacaa tcgtggttaa
    15901 cgcttcgtct ttttccatcg tcgcggccag gacatttgct ggcaaatcaa gataaactcc
    15961 gccagggcga cccgatacag agacccggat agcgcgtgcc aatgcaatgc caagatcctg
    16021 cggctgatta acgcgaaatg ctgctttggc atacggtttt gccgcattca tttggtccag
    16081 ctcttcataa tcaccttgct gtaggtcgac gatcgcgcgg tcgctggagc cgctaatcat
    16141 gatcatcgga aaaccattta ccgttgcgtt ggccaatgcg gtcaaaccat tgaggaatcc
    16201 tggcgcagaa actgtcaggc agatccccgg tttttgggta agaaaaccgc ttgccgcagc
    16261 ggcataacct gccgactgct catgacgaaa accaatataa cgaatgcctt ccgcctgggc
    16321 atggcgtgcc atatccgtca caggaatacc tacaacacca taaatagtgt caatattatt
    16381 ctgttttaat gcttcaacga tgatatgcat accatctgtc atttgaagtt gatctgacat
    16441 gattaataac ctcgaaattt ttataaattg ggagccagaa gcccccgtta atatcagatg
    16501 gcgtggtttt gcttcattgc agcaatttca tcgtcgctat aacccagctc ctgcaataca
    16561 gcagcggtat gttcacctaa tagcggcgca gctttaatat ccggcgtaaa ggcagagaat
    16621 ttcattggac aaccaacggt cagatatttt ccacgcaacg gttgttccac ttcaacaaca
    16681 ctgccacttt ggcgcaaaga gggatcaagt gaaatttctt tcatacttaa aaccggtgca
    16741 caaggaatat caaactgagt caaataggcc accgcttcat gtttatcaat agtgacagtg
    16801 tatttttcga tttcagcaaa aatatcgaaa atatgtggct gtcgggcatg ggctgtactg
    16861 tatgccgaat cggtaatcca ttctggtttg ccgatggctt tacaggtgtt ttcccagttt
    16921 tgctcctgaa tagtgaaata aatataggcg ttaggatcgg tttcccagcc tttacatttc
    16981 aggatccagc caggctgacc gccaccacct gcattaccac cgcggggaac tgcatcacca
    17041 aatgtaccat tcggatactg cgggtattct tccagataac ccaatttatc gagacgctgc
    17101 tggtcacgta atttcacgcg gcaaaggttc aatacggcat cctgcattga catggtgact
    17161 cgttgcccac gccccgtttt ttcgcgatgc agcaaagcag caagtaaacc gatcagcaaa
    17221 tgcattccgg tgttgctgtc acccaacgct gcagcgctta ccagcggcgg accatcccaa
    17281 aaacccgtag tggatgccgc gccacccgct gcctgagcaa cgttttcata ggcttttaca
    17341 ttcacataag gcgaacactc atcaaaccct ttgatcgaac caaaaatcag acgtggattg
    17401 atttcttgaa tatgctccca ggtgaagccc atgtgatcaa tggcccctgg atgaaagttc
    17461 tcgactaaga tatcagcttc gcggatcagc ttttccatta cctctttgcc ttccgctgtt
    17521 ttggtattta actcaataga acgtttgtta ctgttaagca tggtgaagta aagcgcatcg
    17581 atatcaggaa tatctcgcag ttggtgacgc gttacgtcac caacgccggg acgttcaatt
    17641 ttaataacgt cagcgccaaa ccaggccagc atttgagtac aagatgggcc agattgcaca
    17701 ccggtgaaat cgagaacttt aattccttga agtggagttg acatagcaat acctattagt
    17761 taattaaata tgtgttaatt tagcgtttat ttaaggcata acgccctcta taaataatct
    17821 gcaaaatatc ttcgtagcgg cactcgatat ttttaaattc ctgtatcaag tactgaaaat
    17881 tatttcatcg ccacttccaa ttcctttctg catggtaagt agtgaggacg aaaagaagaa
    17941 tatgataaat atttattcgt ggtaatcaga gagagtcgcg acatttgtga tgttattctt
    18001 acaactgtaa gaagcgccgg tgatttacaa gtaagagtat gattacgctt tttaaagcgt
    18061 accataaagc ataacataat aaatgatttg tgatgacact ggattattgc ggtttacttt
    18121 gaggaaaata aaatgcgtgt ttatagaacc gcgagcgcca aataatttta tgaccacaac
    18181 ctgtcgtatt gtggtcataa aaaattgtcg gctggaggga gttaaatcct ttacctcaat
    18241 atactgcctg tcagcattag ttcacaaaca gtgcttcgct atcgacgatg attccatctt
    18301 ccgcaccttt aagcgccagg tttgcctcgt agtattgctt tttatcaagc agtttctgcg
    18361 catcagcaag tgcattacga gtctgtttaa gcggcatcag atattggttt tccattacgc
    18421 caacaccggc caggcgaagc tcttccattg cgcccttttt atcccctttc gccatttttt
    18481 cattggcaat ttttatcgcg gcttcttttt cgggtgttgc tacataactc tcagaaattc
    18541 cgacagatgc attaatgaca atatattggt catcattgag attggtcttt ttacctggct
    18601 tagcgaactt tgcccattcg gtactgtcat ctgacagcaa agcggaagct tcattggtca
    18661 gttcttttgc cttttccgga tcgccatgaa acagagccag gcgcgctacc tgcacgtcac
    18721 gcatcgcata taaaccttgt tcagagatac gctctgctgc tgccgttttc tgggtttgct
    18781 gagtttgctg ttgtgccgct accggtgcat tatcagcagc ccatacagtt gaagatgcca
    18841 gaattgctgt gaccatcgta gccataatta aacgtttcat acttacctca ttctttaaca
    18901 gttttagaaa acgccgccat catattgggt gagcatgaaa tgagaaatca gagagcaacg
    18961 gtcattttga aagggttatc ttacaattgt agttattatc gcaaagcggc atttagtctt
    19021 acagttgtag gaatatgctt cctgtcgcag agaactattc ttatatacca caaagagatg
    19081 tcatttatta tattgatgta aatattgatt aattatatgt aaatacagcc ataacatcgt
    19141 atggttctca gtgaaccatt ttcaggataa ctttctatga aagtaaactt aatacttttc
    19201 agcttatttt tattggtctc tattatggca tgcaatgttt ttgcattttc catttctggt
    19261 ggtgtaagtg agaggagcta taaagagact gaaaaaacat cagcgatgac aaccacacac
    19321 tctacaaaac ttcagccatc acaggcgatt ttgtttaaga tgagagaaga tgcgccacca
    19381 ttaaacctca cagaagaaat cacgcctact tatccgacaa aggcgaatta tcttattcat
    19441 cctgtgcgat agcgtctgtc acatggcagg tgaacaatca tgtctgatgc tgatgtgaac
    19501 acctgcccaa atgagccatc ctgacccact atcgcctgcc ataccccacc ataaagcgaa
    19561 tcatcaagcc acaagccggg gcaagaaaca taaatatccc cgtcaccagt tcattaccgc
    19621 cagttaacca catggtgaag taataaatga gtgcagcgag gatcaccatg ccgaagaaag
    19681 aagagaccgc cgataccacg ttcagtacct gaccgatgta gccgcttacg cagacaatac
    19741 acagggcgag aaacatccat aaataaatca tagctcaacc tcctgccgcg ctaaaaaacc
    19801 tgtaacttca gtcactttaa tagtaagaca atccttaacc tctggcaatt tttgctaagc
    19861 atcaatgaaa acagcacgtt aaatgcaacg ctattgcgtg cggaaaattt tttactttgc
    19921 acgattaata atcagtagct gaaagcagtc agccagtgat aaagtagagc caccgcattc
    19981 atgctcctcg tggttatgtc ctgactagtc tttacactct ttacaggaac cattgtcgta
    20041 catgatggcc caaccaattg agtgtaccgc tgtcgacatg gaatcttctg cgatacaaca
    20101 attcgtatct acagaaggta actatgtttc cacaatgcaa attttcccgc gagtttctac
    20161 atcctcgcta ctggctcaca tggtttgggc ttggtgtact ctggctttgg gtacagcttc
    20221 cttatcctgt tctctgcttt ctcggcacgc gtattggcgc aatggcgcga ccattcctga
    20281 aacgtcgtga atctatcgcc cgtaaaaacc tggaactttg tttcccgcag cattctgcgg
    20341 aagaacgcga gaagatgatt gccgaaaact ttcgttcact cggcatggcg ctggtagaaa
    20401 ccggcatggc atggttctgg cccgacagtc gcgtacgtaa atggtttgat gttgaagggt
    20461 tggataaccc taaacgcgca caaatgcaaa atcgcggcgt aatggttgtc ggcgtccatt
    20521 ttatgtcgct ggaactgggc ggccgcgtga tgggactgtg ccaaccaatg atggctacct
    20581 atcgtccaca taataatcag ctgatggaat gggtgcagac ccgtgggcgc atgcgctcta
    20641 acaaagcgat gatcggcaga aataatctgc gcggcattgt cggtgcactg aagaaaggtg
    20701 aagcggtatg gtttgctccc gatcaggatt atggtcgtaa aggcagctcc ttcgcgccgt
    20761 tctttgcggt ggaaaatgtc gccacaacca atggcaccta tgttctctcc cgtctctctg
    20821 gcgcagccat gttgaccgta acgatggtaa gaaaagcgga ttacagcgga tatcgtttgt
    20881 tcatcacccc agagatggaa ggctacccga cagatgaaaa tcaagccgct gcctatatga
    20941 acaagattat cgagaaagag atcatgcgcg caccggagca gtacctctgg atccaccgtc
    21001 gctttaaaac gcgcccggtg ggagaatcgt cgttgtacat ttaacaaact gctcttactc
    21061 tttccctcct caaatcggcc ataataacct catggttttt atggcttttt tcttaaggtt
    21121 ttttccttcg ctgaatatct aattattacc gtcagaaatt aaactgtcgc cgatatgaga
    21181 cacttgtaag ttactaaaag taaataagaa aatgcctctt gtctcgtttc agttttaggc
    21241 gtacattaac gccgtctcga gcagtttagc tcagaattct ggggtgatgc ccacggtaaa
    21301 atacagggct tttaaggtcg ctttgcccgg tgtcaactca ctgtatcagg tgtaatgaag
    21361 tcattcaggc gtaacagtaa ttacgcggag agatgtaaag tgaaatattt ctttatgggc
    21421 atttctttta tggtcatcgt ttgggccggt acttttgccc tgatgatcta aaagcaaact
    21481 gtgcaatgaa aaacaggagc catcaggctc ctgtttgcat ttctgacgac ggtgtttatt
    21541 ccgcgttttc gtgaatatgt ttgctgctgg cgggtagtaa accgtcggca cggaacatcg
    21601 ctttaatccc tctgatggcc tgacgaatac ggtcgcggtt ttcaatcagt gcaaagcgaa
    21661 catgcgtatc accatagtcg ccaaagccaa tccccggcga gacacaaacc ttcgcttcgt
    21721 taaggagctt cttggcaaat tccagcgatc ccatggccgc atatggttcc gggattttcg
    21781 cccagacata catcgaagcc ttcggcattt cgaccatcca gcccgcttca tgcagccctt
    21841 taaccaatac atcacggcgg cgtttgtact gttcggtaat gtcgcgcacg cactgttgat
    21901 cgccctcgag tgccgcaatc gccgccacct gcaacggcgt aaaagtgccg taatcgtgat
    21961 agcttttgat tcgcgccagg gcactgacca gcgttttgtt gccgaccata aagccgatac
    22021 gccagcccgc catgttgtag cttttcgaca gcgtaaagaa ctcgaccgcc acatcgcgtg
    22081 cacccggcac ctgcataatt gacggcgctt tccagccatc gtagacgata tcggcatagg
    22141 ccaggtcatg gaccaccagc acatcgtagc gtttcgccag cgccacaacc ttttcgaaga
    22201 actccagttc cacgcattgc gcggttgggt tcgacgggaa gccgaggatc atcatcttcg
    22261 gtttcggata actttcacga atggcgcgct ccagttcgtt gaagaaatcg acaccttcca
    22321 ccagcggcac tgagcgtacc tgcgccccgg caatcaccgc gccataaata tggatcgggt
    22381 aacttggatt cggcaccagc accgtgtcac catgatccag cgtcgccagc atcagatgcg
    22441 ccaggccctc tttcgaacca atagtgacga tggcttctga ttccgggtcg atttcaacgt
    22501 cgtagcgatc ctgataccag cgggaaatgg cgcgacgtaa ccgcggaatg ccgcgtgaag
    22561 tggagtaacc atgcgtgtcc gggcgctggg ccacagtaca taatttttcg acgatatgcg
    22621 gcggagtcgc accgtccggg ttacccatgc tgaaatcgat aatatcttcg ccgcgccgac
    22681 gcgcagccat tttcagttca gcggtgatgt taaaaacgta gggcgggaga cgatcaatgc
    22741 gcgtaaagcg acgttcaggg cgagtgtcag ccataatttc ctcagattaa cgttagcgcc
    22801 cggaccgtcc gagcgacgct gccacaatgg tggctgtttt gaaaatagcc tgattaattt
    22861 ctgtctgtcc agcgttttag aacattttta tcaatggctt taaatgagga actccacttc
    22921 aaaaacagga aatttttcta cgctaacaag aaaattacca ccaattttat taacattata
    22981 atttcaataa atttaccagc cagaagtgca ttatcaagag atacattgct aatacaccat
    23041 ttatctttca atcccctttt aaatttatgg tttttcctca aaaacggcct gctggtcatc
    23101 agccttcagg ttacctatca tagaggttta atccttattc agagtcaccc gtgcacgaaa
    23161 tattcaacat gctgctggcg gtcttcgatc gggcagcgtt aatgcttatc tgcctgttct
    23221 ttctcatccg tatccgcctg tttcgcgaac tgttgcacaa gtcggcgcac tccccaaaag
    23281 aattgctcgc cgttaccgcc attttctcgc tgttcgccct gttcagcacc tggtccggcg
    23341 ttcccgtaga aggctcgctg gtgaacgtac gtattatcgc ggtgatgtcc gacgggattc
    23401 tgtttggccc gtgggtaggc atcattaccg gcgtgattgc gggtattcac cggtatttaa
    23461 ttgatatcgg cggcgtgacg gcgatcccct gctttatcac cagcattctg gcgggttgta
    23521 tatcaggctg gataaacctg aaaatcccca aagcacagcg ctggcgcgtc ggtattctcg
    23581 gcggcatgtt gtgtgagacg ttgaccatga ttctggtaat tgtctgggca ccaactaccg
    23641 cgttggggat cgatatcgtc tctaaaatcg gcattccaat gatcctcggt agcgtctgta
    23701 tcggctttat tgtgcttctg gtgcaaagcg ttgagggcga aaaagaggcc agcgccgcgc
    23761 ggcaggccaa gctggcgctg gatatcgcca acaaaacgct accgctgttt cgccatgtca
    23821 atagcgagtc attacgcaag gtctgcgaaa ttatccgcga tgacattcac gccgatgcgg
    23881 tggcgattac taataccgat catgtgctgg cctatgttgg cgtgggtgaa cataactatc
    23941 agaatggcga tgacttcatt agcccgacta cccgtcaggc gatgaattac ggaaaaatca
    24001 tcattaaaaa caatgatgaa gcccaccgca caccagagat tcattccatg ctggtgatcc
    24061 cattgtggga gaaaggggtc gtgaccggaa cgctgaaaat ttactactgc cacgcgcatc
    24121 agatcacctc gtcattacag gaaatggcgg tcggtctgtc gcaaatcatc tccacgcaac
    24181 tggaggtttc acgcgccgag cagctacgtg aaatggcaaa taaggcagag cttcgcgccc
    24241 tgcaaagcaa aattaatccc cattttctgt ttaacgctct gaacgctatt tcatcgtcaa
    24301 tccgtctgaa tccggatacc gctcgccagt tgatctttaa tctgtcgcgt tatctgcgct
    24361 ataacattga attaaaagac gatgagcaaa tcgatatcaa aaaagagctg tatcaaatta
    24421 aagactatat tgccattgag caggcccgct ttggtgacaa gctgacggtt atctatgata
    24481 ttgatgaaga ggtgaattgc tgcattccca gcctgctgat ccagccgttg gtggagaacg
    24541 ccattgtcca cggtattcag ccttgcaaag gtaaaggcgt tgtcaccatc agcgttgcag
    24601 agtgcggaaa tcgggtacgc attgcggtgc gagataccgg gcacggcatc gatccaaagg
    24661 tgattgagcg ggtcgaagcg aatgaaatgc cgggcaataa aattggcctg ctgaatgtcc
    24721 atcatcgcgt gaagttattg tatggcgagg ggctgcatat ccgccgcctg gagccgggga
    24781 cggaaattgc gttttacatt cctaaccaac gcaccccagt cgcctcacag gctacgttat
    24841 tgctttgagc cggagtgata ttgtgaaagt catcattgtt gaagacgaat tcctggcaca
    24901 acaggaactg agctggctaa ttaaagagca cagccagatg gagattgtcg gcacctttga
    24961 cgacggtctg gacgtgttga agtttttgca gcataaccgc gtcgacgcca tttttctgga
    25021 tatcaatatt ccgtcgctgg atggcgtgtt gctggcgcaa aacatcagcc agttcgccca
    25081 taaaccgttt attgtgttca tcaccgcgtg gaaagaacat gcggtagaag cgtttgaact
    25141 ggaggcgttt gactacattc tcaaaccgta tcaggagtca cgtattaccg ggatgctgca
    25201 aaaactggaa gcggcctggc aacaacagca gaccagcagt acgcctgccg cgacggtaac
    25261 gcgtgagaat gacaccatta atctggt
//
LOCUS       DAKISA010000058        24804 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.058, whole genome shotgun sequence.
ACCESSION   DAKISA010000058 DAKISA010000000
VERSION     DAKISA010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24804
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.058"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..796)
                     /locus_tag="OVW45_002462"
     CDS             complement(<1..796)
                     /locus_tag="OVW45_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCV5526885.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKNGNKLDLYGKVDARHTF
                     SDNPGDDGDETIIELGFKGETQITDQLTGYGQALTKTKASDTEGSDNTYVKLAFAGLK
                     FGEMGSFDYGRNYGVIYDVEAWTDMLPVFGGDSYTWTDNFMAGRANGVATYRNSDFFG
                     LVEGLNFALQYQGNNEGSNAGEDQEGTKNGHEDVRFQNGDGFGLSTSYDFDFGLSLGA
                     AYSNSDRTNSQVALGGYHYNEYSKFAGGDTAEAWTFGAKYDANNVYL"
     gene            1363..1728
                     /locus_tag="OVW45_002463"
     CDS             1363..1728
                     /locus_tag="OVW45_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCV5526886.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGSIAWYTRPRPARSRRYE"
     gene            1768..2424
                     /locus_tag="OVW45_002464"
     CDS             1768..2424
                     /locus_tag="OVW45_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HCV5526887.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAQKLAADDDVDMLVILTACYF
                     HDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCHAIAAHSFSAQIAP
                     LTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQHRPLDDKRYALDH
                     FQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEGVDHKVIDAFSPAG
                     "
     gene            2491..3909
                     /gene="dcm"
                     /locus_tag="OVW45_002465"
     CDS             2491..3909
                     /gene="dcm"
                     /locus_tag="OVW45_002465"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCV5526888.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPSAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            3890..4360
                     /gene="vsr"
                     /locus_tag="OVW45_002466"
     CDS             3890..4360
                     /gene="vsr"
                     /locus_tag="OVW45_002466"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCV5526889.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(4349..5270)
                     /gene="yedA"
                     /locus_tag="OVW45_002467"
                     /pseudo
     CDS             complement(4349..5270)
                     /gene="yedA"
                     /locus_tag="OVW45_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
     gene            5443..6360
                     /locus_tag="OVW45_002468"
     CDS             5443..6360
                     /locus_tag="OVW45_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCV5526890.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            6439..6621
                     /locus_tag="OVW45_002469"
     CDS             6439..6621
                     /locus_tag="OVW45_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCV5526891.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            6792..8486
                     /gene="yedQ"
                     /locus_tag="OVW45_002470"
     CDS             6792..8486
                     /gene="yedQ"
                     /locus_tag="OVW45_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HCV5526892.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNTSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(8483..9298)
                     /locus_tag="OVW45_002471"
     CDS             complement(8483..9298)
                     /locus_tag="OVW45_002471"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HCV5526893.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPTALWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(9596..9823)
                     /gene="yodD"
                     /locus_tag="OVW45_002472"
     CDS             complement(9596..9823)
                     /gene="yodD"
                     /locus_tag="OVW45_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCV5526894.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            9986..10174
                     /gene="dsrB"
                     /locus_tag="OVW45_002473"
     CDS             9986..10174
                     /gene="dsrB"
                     /locus_tag="OVW45_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCV5526895.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            complement(10218..10841)
                     /gene="rcsA"
                     /locus_tag="OVW45_002474"
     CDS             complement(10218..10841)
                     /gene="rcsA"
                     /locus_tag="OVW45_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCV5526896.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(11126..11911)
                     /gene="fliR"
                     /locus_tag="OVW45_002475"
     CDS             complement(11126..11911)
                     /gene="fliR"
                     /locus_tag="OVW45_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCV5526897.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFVIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            complement(11920..12189)
                     /gene="fliQ"
                     /locus_tag="OVW45_002476"
     CDS             complement(11920..12189)
                     /gene="fliQ"
                     /locus_tag="OVW45_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCV5526898.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            complement(12199..12936)
                     /gene="fliP"
                     /locus_tag="OVW45_002477"
     CDS             complement(12199..12936)
                     /gene="fliP"
                     /locus_tag="OVW45_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HCV5526899.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     SVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            complement(12936..13301)
                     /gene="fliO"
                     /locus_tag="OVW45_002478"
     CDS             complement(12936..13301)
                     /gene="fliO"
                     /locus_tag="OVW45_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCV5526900.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            complement(13304..13717)
                     /gene="fliN"
                     /locus_tag="OVW45_002479"
     CDS             complement(13304..13717)
                     /gene="fliN"
                     /locus_tag="OVW45_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCV5526901.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            complement(13714..14718)
                     /gene="fliM"
                     /locus_tag="OVW45_002480"
     CDS             complement(13714..14718)
                     /gene="fliM"
                     /locus_tag="OVW45_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCV5526902.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDGIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            complement(14723..15187)
                     /gene="fliL"
                     /locus_tag="OVW45_002481"
     CDS             complement(14723..15187)
                     /gene="fliL"
                     /locus_tag="OVW45_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCV5526903.1"
                     /translation="MTDYAISKKSKRSLWLPILVFITLAACASAGYSYWHSHQVAAED
                     KAQHRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            complement(15292..16419)
                     /gene="fliK"
                     /locus_tag="OVW45_002482"
     CDS             complement(15292..16419)
                     /gene="fliK"
                     /locus_tag="OVW45_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCV5526904.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALASETTTD
                     KAAPQVLVATDKPTTKGELLISDIVSDAQQADLLIPVDETLPVINVEQSTSTPLTTAH
                     TMTLAAVADKNTTKDEKADDLNEDLTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLLTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEEDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            complement(16416..16859)
                     /gene="fliJ"
                     /locus_tag="OVW45_002483"
     CDS             complement(16416..16859)
                     /gene="fliJ"
                     /locus_tag="OVW45_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HCV5526905.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            complement(16878..18251)
                     /gene="fliI"
                     /locus_tag="OVW45_002484"
     CDS             complement(16878..18251)
                     /gene="fliI"
                     /locus_tag="OVW45_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCV5526906.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            complement(18251..18937)
                     /gene="fliH"
                     /locus_tag="OVW45_002485"
     CDS             complement(18251..18937)
                     /gene="fliH"
                     /locus_tag="OVW45_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCV5526907.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            complement(18930..19925)
                     /gene="fliG"
                     /locus_tag="OVW45_002486"
     CDS             complement(18930..19925)
                     /gene="fliG"
                     /locus_tag="OVW45_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCV5526908.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTYVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            complement(19918..21576)
                     /gene="fliF"
                     /locus_tag="OVW45_002487"
     CDS             complement(19918..21576)
                     /gene="fliF"
                     /locus_tag="OVW45_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HCV5526909.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYCFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            21792..22106
                     /gene="fliE"
                     /locus_tag="OVW45_002488"
     CDS             21792..22106
                     /gene="fliE"
                     /locus_tag="OVW45_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCV5526910.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            22440..22772
                     /gene="emrE"
                     /locus_tag="OVW45_002489"
     CDS             22440..22772
                     /gene="emrE"
                     /locus_tag="OVW45_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCV5526911.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            22941..23492
                     /gene="ybcL"
                     /locus_tag="OVW45_002490"
     CDS             22941..23492
                     /gene="ybcL"
                     /locus_tag="OVW45_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCV5526912.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGV
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            23502..24299
                     /locus_tag="OVW45_002491"
     CDS             23502..24299
                     /locus_tag="OVW45_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCV5526913.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQNLYAITDQIR
                     NTTGFCLPQNSELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
BASE COUNT         6102 a         6131 c         6346 g         6225 t
ORIGIN      
        1 caagataaac attgttggcg tcgtattttg caccaaatgt ccatgcttca gctgtatcac
       61 caccagcaaa tttgctgtat tcattgtagt gatacccgcc aagagctact tgagaattgg
      121 tacggtcaga gtttgagtaa gcggcaccta agctcaaacc gaagtcaaag tcatatgaag
      181 tggaaagacc aaaaccatca ccgttctgga agcgaacgtc ttcgtgacca tttttagtac
      241 cttcttgatc ttctcctgca ttgctgcctt cgttgttacc ttgatactgc aatgcaaagt
      301 tcagaccttc caccagaccg aagaaatcac tattgcggta agtagctacc ccatttgcac
      361 ggcctgccat gaagttatca gtccaggtat aggagtcgcc gccgaataca ggaagcatat
      421 ctgtccaggc ttcaacgtcg taaatcacac catagttacg accatagtca aaggagccca
      481 tctcgccgaa cttcagacct gcaaaagcaa gtttgacata agtattatca gaaccttcag
      541 tgtcgctagc ctttgttttg gtcagtgctt gaccataacc agttaactga tctgtaattt
      601 gggtctcacc tttgaagcct aactcgatga tagtttcatc gccgtcatcg ccagggttat
      661 cggaaaaagt atgacgagca tcaacttttc cgtacagatc cagtttattg ccatttttat
      721 tataaatttc agccgcattt gctgcgccag caactaataa cgctgggacc agcattgcca
      781 gaacttttct tttcattatg tattcccttg tgattataat cttcatgaat atatcaataa
      841 gtgccgttat ccaaaaaaaa agcacatttg gatactattc tatgaagttc attttatttt
      901 aaagattgcg tgtaacaaat gtatttaaaa gatttcaaaa tgtttctaat ctatttaata
      961 taattcagaa ttatgaattc taaatataaa catccatgaa aattataaat gtatatcaat
     1021 atgttatgag ttgaatggaa agatagggag tatcaatgat tgttgcgcca aacggcaggc
     1081 gtgatcacgc gcacattaat gaaggtataa aaaaagccgg catcatgccg gcccaaaaaa
     1141 tataaacaag acgataaaag gctaataacg gggttatcat gacacagaaa tcaaaaaagg
     1201 tataattgta ttcagtgcta atatattgtt tgggtgaatt aataaaacta ttaccaagga
     1261 tataaataaa tctttattgc tgtttttcca gtaattattg ccgttgcgca aaatttatct
     1321 atttgttcaa aaaatgattg agtcttgact ggctcatcca atgtggaaaa atgtgacttt
     1381 tatcacataa tagtactaag tctgaatttt ccgggttatc tcaaaatgga atacggttcg
     1441 acaaagatgg aagagagact ctctcgcagc cctggcggca aattggcttt gtgggctttc
     1501 tatacatggt gtggctattt cgtctgggca atggcgcgtt acatctgggt gatgagccgg
     1561 atccctgatg ccccggtgtc gggatttgaa agcgatttag gctctaccgc gggaaagtgg
     1621 ttaggggcac tggtcggatt tttatttatg gctctggtcg gggcattgtt aggcagcatt
     1681 gcctggtaca cccgacctcg tcccgcacgt agcagacgct atgaataaaa actgctaagg
     1741 tggcgactct ttggcaacgg agcgcgtatg gacttacaac actggcaggc acagttcgaa
     1801 aactggttga aaaaccatca tcagcaccag gacgcggcac agaagctggc ggcagatgac
     1861 gatgtcgata tgctggtgat tttaaccgcc tgttattttc acgatattgt gagcctggcg
     1921 aaaaatcatc cgcaaaggca gcgttcttca atcctggcgg cagaagagac gcgtcgcctg
     1981 ctccgtgaag agttcgagca atttccggcg gagaaaatcg aggccgtttg tcatgccatt
     2041 gccgctcaca gtttcagcgc gcaaatcgcc cccttaacaa cggaagctaa aatcgtccag
     2101 gatgcggatc ggctggaagc cctgggggcg attggcctgg cgcgcgtgtt tgccgtttca
     2161 ggggcattgg gcgtggcgct gtttgatggt gaagatccgt tcgcacagca tcgcccgctt
     2221 gatgataaac gctacgcgct ggatcatttc cagactaagt tgctgaagct accgcaaacc
     2281 atgcaaaccg caagaggcaa gcagctggcg cagcacaacg cgcatttttt agtcgagttt
     2341 atggcgaagc tcagtgccga actggcgggg gagaatgaag gtgtcgatca caaggtgata
     2401 gatgcgtttt cacccgcggg ataagcgcgt ggccctaaat ggctgtaatt atgttaacct
     2461 gtcggccatc tcagatggcc ggttaaatct atgcaggaaa atatatcagt aaccgattca
     2521 tacagcaccg ggaatgccgc acaggcaatg ctggagaaac tgctgcaaat ttatgatgtt
     2581 aaaacgctgg tggcgcagct taatggtgtg ggtgagaatc actggagcgc ggcaatttta
     2641 aaacgtgcgc tggcgaatga ctcggcatgg caccgtttaa gtgagaaaga gttcgcccat
     2701 ctgcaaacgt tgttacccaa accatcggca catcatccgc attatgcgtt tcgctttatc
     2761 gatctatttg ccggaattgg cggcatccgt cgcggttttg aatcgattgg cggacaatgc
     2821 gtgtttacca gcgaatggaa caaacatgcg gtacgcactt ataaagccaa ccattattgc
     2881 gatccggcga cgcatcattt taatgaagat atccgcgaca tcaccctcag ccataaagaa
     2941 ggcgtgagtg atgaggcggc ggcggaacat attcgtcaac acattcctga acacgatgtt
     3001 ttactggccg gtttcccttg tcagccattt tcgctggctg gcgtatcgaa aaagaactcg
     3061 ctcgggcggg cgcacggttt tgcctgcgat acccagggca cgctgttttt tgatgtggta
     3121 cgcattatcg acgcgcgtcg tccggcgatg tttgtgctcg aaaacgtcaa aaacctgaaa
     3181 agtcacgacc agggtaaaac gttccgcatc atcatgcaga cgctggacga actgggctat
     3241 gacgtggctg atgcagaaga taacgggccg gacgatccga aaatcatcga tggtaaacat
     3301 tttctgccgc agcaccgtga acgcatcgtg ctggtgggtt ttcgtcgcga tcttaatctg
     3361 aaagccgatt ttactctgcg tgatatcagc gaatgtttcc ctgcacagcg agtgacgctg
     3421 gcgcagctgt tggacccgat ggtcgaggcg aaatatatcc tgacgccggt gctgtggaag
     3481 tacctctatc gatatgcgaa aaaacatcag gcgcgcggta acggcttcgg ttatggaatg
     3541 gtttatccga acaatccgca aagcgtcacg cgtacgctgt ctgcgcgtta ttacaaagat
     3601 ggcgcggaaa ttttaatcga tcgcggctgg gatatggcca cgggtgagaa agattttgac
     3661 gatccgctga atcagcaaca tcgtccacgt cggttaacgc ctcgggaatg cgcgcgctta
     3721 atgggttttg aagcgccggg agaagcgaaa ttccgcattc cggtttcgga cactcaggcc
     3781 tatcgccagt tcggtaactc ggtggtcgtg ccggtctttg ccgcggtggc aaaactgctt
     3841 gagccaaaaa tcaaacaggc ggtggcgttg cgtcagcaag aggcacaaca tggccgacgt
     3901 tcacgataag gccactcgca gcaaaaatat gcgcgcgatt gccacgcgtg atacggcgat
     3961 tgagaagcgc ctcgccagtc tgttaaccgg gcagggcctg gcgtttcgcg ttcaggacgc
     4021 cagtctgccc ggaagcccgg attttgtcgt tgatgaatat cgctgcgtga tatttaccca
     4081 tggctgcttc tggcatcatc atcactgcta tctgtttaaa gtgcctgcga ctcgaaccga
     4141 gttctggctg gagaagatag gtaaaaatgt tgaacgcgat cgccgcgata tcagtcgttt
     4201 gcaggaactc ggctggcgcg tactgattgt ctgggagtgt gcattacgtg ggcgcgagaa
     4261 gctgacggat gaagcgctta ccgagcgtct ggaagagtgg atctgcggcg aaggtgccag
     4321 cgcgcagatc gacacgcagg ggattcattt actcgcttga tgcgtcctga ataactggcg
     4381 caactacggg ttttgccggg aagagatatt ttcccaacgt gaccagtacc accgcgaaga
     4441 caattacgcc gagcgccagc cattcaatct tcgacagcgt ctctccgccc agtcccgtac
     4501 ccagcaagac cgcgaccacc gggttaacgt aggcgtagct ggtggcgaga gccggactga
     4561 cattacggat taaatacata taagcgttga tggcgataat cgaaccaaac agcgccagat
     4621 agccgaccgc aaggaagcct gaaagggaag ggagcgccgt cagtttttca cctgcaatca
     4681 tcgacgcgat cattaacacc acgcctgccg ccagcatctc aatcgcaccc gccatcatcc
     4741 ctacaggtaa ggtaatgcgc gagccataaa ccgagccaaa cgcccagcta atcgagccga
     4801 ttaaaatcag aatcgcgccc cacggattgc cgcttaaatt tccaccgcta ttgagcatga
     4861 tgattccggc aagcccaatg gcgataccca cccattccag tttgcgcgtt ttaatgccaa
     4921 acaggcggct gaagcaccag ggtaaagagg ggcacggttg caaccacaac ggcggcgatg
     4981 ccggaaggaa cattttgatg ctcggcaacc gtcaccatgc cattaccgac agccagcaat
     5041 aacaggccaa tcagcgcggc attgagcagc ggacgtagcg gggggagctt gtgtccgcgc
     5101 agtagcaaaa atgccatcaa taaaataccg gctgccagga atcgaacgcc tgccatcatt
     5161 aacggaggcc agctttccac gccaatccga atgacaaaat aggttgagcc ccaaatgata
     5221 tacaacgcaa acagcgcgcc aaaaagcggt aacaactggc ggaaacgcat aatccctcac
     5281 ggtggaaata aaaaggtggt tcatagtaaa cgtgaaaatc attctgctgg cgagagatat
     5341 aattgcactt gattgtaaaa aaaatgttga cctgtgaagc aagtatcaga gctgccgttt
     5401 ttgcttcata cttacaccct tcaacaataa aaatgagagg gaatgctttt ggccgggagt
     5461 agtttactga cgttgctcga tgatatcgcc acactactgg acgatatctc cgtgatgggc
     5521 aaactggcgg cgaagaaaac cgccggtgta ttaggggatg acttatcgct caatgcgcaa
     5581 caagtttcag gcgtgcgggc caaccgggaa cttcccgtgg tctggggcgt ggcgaaagga
     5641 tcgctgatta ataaagtgat tctggtgccg ctggcgctga tcatcagtgc gtttatcccg
     5701 tgggcgatta cgcctctgtt gatgattggt ggcgcgtttc tctgctttga aggagtagag
     5761 aaagtgctgc atatgctgga ggcgcgtaaa cataaagaag atccggcgca gagccagcag
     5821 cgtctggaga agctggcggc gcaggatccg ctgaagtttg aaaaggacaa aataaaaggg
     5881 gcgattcgta ccgattttat attgtctgcc gaaatcgtcg ccatcacgct ggggattgtg
     5941 gccgaagcgc cgttgcttaa tcaggtgctg gtgctttcag gcatcgcgct ggtagtgacc
     6001 gtgggcgtct acggtctggt aggggttatc gttaagattg atgacctggg gtattggctg
     6061 gcggaaaaat ccagcgcgct gatgcaggca ttaggtaaag ggttattgat cattgcgccc
     6121 tggttgatga aggcgttatc gattgtcggc acgctggcga tgttcctcgt cggcggcggt
     6181 attgtggtac atggtattgc gccgctgcat cacgccattg aacatttcgc cgggcagcaa
     6241 agtgcagtgg tggcgatgat attaccgact gttttaaatc tgattcttgg atttatcatc
     6301 ggcggcatcg tggtgctggg agtgaaagcc gtagcgaaaa tgcgcggtca ggcacattaa
     6361 tactcgcaca ggctattatg caaaattcgc catcttcaac taaggtgaac aagtttcact
     6421 cgaaaaaagg aggcgagtat gagctttatg gttagtgagg aagttacggt taaagagggc
     6481 ggcccgcgga tgattgtcac tggatactcc agcggtatgg ttgagtgtcg ttggtatgac
     6541 ggttacgggg tcaagcggga agcttttcat gaaaccgagc ttgttccggg ggaggggagt
     6601 cgttctgcgg aagaagtttg aaagggtatc attaacacca ggccggatca cttccggcct
     6661 ggtgtgccaa tgcctgcaat gaatgatagt cgcgtcttat catgccacca ttataaaatt
     6721 atcgctgttt tgcggtacgc tatcgccagg ttatgccaga atcataaaaa agcaggttgg
     6781 gagtcgtcag ggtgcagcac gagacaaaaa tggaaaacca gagctggttg aaaaaactcg
     6841 cacgccgctt ggggcctggt catgtcgtta atctctgctt tatcgtggta ttgctttttt
     6901 ccaccttgct gacctggcgt gaagtggtag tgctggaaga tgcctatatc tccagccagc
     6961 gtaatcatct ggaaaacgta gccaacgcgc tcgataagca tttgcagtat aacgtcgaca
     7021 aactgatctt tttgcgtaat ggcatgcgcg aagctctcgt agcgccactg gatttcactt
     7081 cactgcgtaa tgctgtaacc gagttcgaac agcatcgcga cgagcacgcc tggcaaattg
     7141 aactcaaccg acgacgcacc ctgtcagtca atggcgtatc ggatgcatta gtcagcgagg
     7201 ggaatctcct gtctcgcgaa aatgaaagcc tcgacaatga aattaccgct gcactggaag
     7261 ttggttactt gctgcgactg gcgcacaaca cctcgtcgat ggttgaacag gcgatgtatg
     7321 tctcgcgtgc cggattttac gtttcgacgc agccgacctt gtttacgcgc aatgtaccaa
     7381 cgcgttatta cggctatgtc acccaaccct ggtttatcgg ccattcgcaa cgagaaaatc
     7441 gtcaccgcgc ggtacgctgg tttacttcgc aaccggaaca cgccagcaat actgaaccgc
     7501 aggttaccgt cagtgttccg gtagacagta ataactactg gtatggcgtg ctggggatga
     7561 gtattcccgt gcgtaccatg cagcaatttt taagaaacgc catcgataaa aacctcgatg
     7621 gtgagtatca gctctatgac agtaagctga gatttttgac ctcttccaat cctgaccatc
     7681 caacagggaa tatttttgat cctcgtgaac tggccttgct ggcgcaggcg atggaacatg
     7741 acacgcgggg cggcattcgt atggacagtc gctatgttag ctgggaacgt ctggaccatt
     7801 tcgacggtgt gctggtgcgc gtccatacgc taagcgaagg cgtgcgcggc gatttcggca
     7861 gtatcagcat tgcattaacc ctgctgtggg cgctctttac caccatgtta ctcatctcct
     7921 ggtatgtgat tcgccggatg gttagcaaca tgtatgttct gcaaagctcg ttgcagtggc
     7981 aggcgtggca cgacacctta acccgtttat ataatcgtgg cgcactgttt gaaaaagccc
     8041 gtccgctcgc gaaattgtgt cagacgcacc aacatccttt ttctgtcatt caggtcgacc
     8101 ttgaccattt taaagcgatt aatgaccgct ttggtcatca ggcgggcgac cgtgttcttt
     8161 ctcatgctgc cggattaatt agcagttcct tgcgtgcgca ggacgttgcc gggcgggtcg
     8221 gtggtgagga gttttgtgtg attctgccag gcgcgagtct gacggaggct gcggaagtcg
     8281 cagaacgtat tcgcctgaag ttaaatgaaa aagagatgtt gattgctaag agtacgacga
     8341 tacgcatcag tgcctcgttg ggggtaagta gcagcgagga aaccggtgat tatgattttg
     8401 aacaactcca gtcactggct gaccgtcggc tttatctcgc taaacaggcc gggcgtaatc
     8461 gggtatgcgc gagcgataac gcttaacgag cggagaaaaa atggtccagc ccttcacgcc
     8521 agccttccgg tccttcacgc tgcgttcgcc agacgcgggt cggatcctca tcatgcagat
     8581 gcaccccttc acggtttagc cctttcacaa tcaccgcgta atccattacc tccagtaagg
     8641 gcgcatcgtt tggcccatcg cccaggccaa gtgtggttgg gcgtttgcct gataattgtt
     8701 gataggtcgc gataatccag ttggcggcct gatcttttcc ggcagaggca tccaggacgt
     8761 gccagaagcg cgcaccctgc ataaactgta agcccagttc gttcagacga gcggtaaatt
     8821 gtgccatacg ctcgtcactg tcgcgccaga ttagcgttac cgacgcctca tgcagctgcg
     8881 tcagcgccgc ctggctacgg cttaatcccg tccattcggc gatggttgca tcgtcgacat
     8941 catcgaaagt cgtgaattta aaatgttctt tctcgcgtag cgtatttaaa acctggctga
     9001 tttcgccatg gctaatacct gagatgatgc gtggaaaacc gtctatatcc tgccattgtt
     9061 cagcaagctg gatcactgcg ccattttcgg caatcagtgg taacccttgt agccccaacg
     9121 ttttttgcaa gtacagcatt tccgctgaag ttttactgct acagagaatg acgggaacat
     9181 tcgcttcgcg taaacggctg agccagaggg cagtcggttg ccagtcataa ctatgactgt
     9241 ccagcagggt gccatcaaga tcgctaaaaa ccagtagtgg ttgttgaatt gaaaacatgg
     9301 tcgtgcagtt ctccccttac caggatgatt ctcaaaacag cacaaataat caaagatgat
     9361 tttttcggga gcgatcccgt tgacgccaga gccaccaagg ggtgacgcac gtcacatttc
     9421 tattcataag tagcgttaat tattcatatc gcgaatattt tcttgtcagc gaaaaaaatt
     9481 gcggataagg tgatgaacac atcagatttc ctggtgtaac gaatttttta agtgcttctt
     9541 gcttaagcaa gtttcatccc gaccccctca gggtcgggat ttttttattg tgcattcaac
     9601 gattcacttc agagacgctg aagtcatgaa tatccagttc gaaggcatcc gccaattcac
     9661 gccatgtatg atattcacgt ccatcgacgc taacgtgttc agaatcgttc tggctgcgat
     9721 gaacttcgct ttcgctaatt tcattgatcg ccgccagcag ggcatcgaca tcgacgctga
     9781 cctcacgttt tgcggtatcg ctgtactctt ttgcggtttt catcatcttt cctcgcaacc
     9841 gttactctgt tgttgccgcc agtcgttaag tccgcctggc atctcttaag tatagaccgt
     9901 cgagaaaatc agcactcgcg cgcgcctgga gctgccagaa aaacgaaatt gttctacact
     9961 ggcacaaagc cacaggagga aaacgatgaa ggtgaatgat cgggtaacag tcaaaacgga
    10021 tggcggtccg cgtcgtcctg gcgtggtact ggcagttgag gagtttagtg aaggcacaat
    10081 gtacctggtt tcgctggaag actacccgct cggcatctgg ttctttaatg aagcagggca
    10141 tcaggacggt atctttgtgg agaaagcaga gtaatcttat tcagcctgac tggtgggaaa
    10201 ccaccagtca gaatgtgtta gcgcatgttg acaaaaatac cattagtcac attatccgtc
    10261 agtcggacga catggtagat aacctgttta ttatgtgttt tgatcttacg tttaatatta
    10321 cctttatgcg atgaaacggt cttggctttg atattcattt ggtcagagat ttgaatggtt
    10381 ccctgacctg ccatccacat tcgcaacata ctcgattcgg ttcggctcaa tgataacgtc
    10441 ggcatattta aaaaagaggt tatcgttgtc tcttttttca gaatatcgcc aaggatatcg
    10501 tcgagagatt ccggtttaat cgatttagaa cttatcaata aattttttct gaccaataga
    10561 tattcatcaa aatgaacatt ggcaattgcc ataaaaacga taaataacgt attgggatgt
    10621 tgattaatga tgtgcttgat gtgctgactg ttagaagcat catggatgaa acagtcctca
    10681 ttaataaaca ccactgaagg gcgctgtgaa tcacaagcta tggcaaggtc atcaacggtt
    10741 tcaatgtcgt tgatttctct ttttttaacc cctctactca acagataccc ggttaaacct
    10801 agtcgggtgt aactacataa atccataata atcgttgaca tggcataccc tcactcaatg
    10861 cgtaacgata attcccctta cctgaatatt tcatcatgac taaacggaac aacatgggtc
    10921 acctaatgcg ccactctcgc gatttttcag gcggacttac tatcccgtaa agtgttgtat
    10981 aatttgcctg gaattgtctt aaagtaaatg ttgcgatatg tgagtgagct taaaacaaat
    11041 atttcgccgc aggagtatcc tggaagatgt tcgtaggtat tatttttcgg ataatcctta
    11101 ggataacatg ataaacgtta cggaattata ttaatggcaa ttcactaata atatcagcca
    11161 gcaaattaaa catctcactg aataaatgtt cgcaaaaagg tgcaattaac ggcattaatg
    11221 ccgccattaa agagataccg acagtcagag ttaatggaaa tccaataaca aaaatggata
    11281 attgcggggc catacgattc agtaaaccta atgccagatt cagggtcagc agcagagtaa
    11341 tgagcggtaa cgccagcatc agcccgttaa ggaaaatcaa actccccgct ttggtgagtg
    11401 ccagaaacgc attgctgttc aacggttcgc caccaattgg cagggtgtga aaggtatcga
    11461 ccagcagtga aatcaaccat aaatgaccgt taaatgtcag gaacagcagt aacgccagca
    11521 tatccatgat acgcgctaaa acgggcatat tgagatggct ggccggatcg acaaacgtcg
    11581 caaatgacag ccccatttgc agaccgataa tttcgccagc ggttcgcaca gcggcaaagg
    11641 caaattgcat ggtaaaacca agcgcaatgc cgatcaggat ctgctgcacg gccagccaca
    11701 gagcaaagaa cgaaaaaaca ggaacatcgt tggcaggtaa tgatggggca atgacgaacg
    11761 tgatcatcat tgccagcccc agttttaccc gtttcggcac gctgcgttcg ctcagaattg
    11821 gtgcggtaga aatgagcgcc agcacgcgca gtaacggcca gaagtacagg cttaaccagg
    11881 aaagccattg atcgcttgtc acctgcatca ttgtacggcc tacccgatga tatacggcag
    11941 gttagtgaac aaggtgcgga cgtaatccag caacagattg agcatccacg gtccggcaat
    12001 aataatggca ataaatacgg cgatgatttt tggaataaac gacagcgtca tttcgttgat
    12061 ctgcgtggcg gcctgcaaaa tactgatgat aaggcccgtg accagcgcca ccagcaacag
    12121 cggggcagcc agtgccagcg cgactttcat cgcttcagtc cccatcatca tgaccgattc
    12181 aggtgtcatt ttgcctctct agctgtaaaa gctctgcgcc agcgaaccga ccagcaattg
    12241 ccagccatcc accagtacaa acagcatcag tttaaagggc agagcaatgg tggcgggggg
    12301 gaccatcatc atccccaatg ccatcaacac gctggcaatc accaggtcga taatcaaaaa
    12361 aggaatgaaa atcgtgaagc ctatctgaaa tgcggttttc aattcgctgg tcacgtaggc
    12421 cgggagcaaa atgcgcatcg gcacggcttc aggtccctgc aacgggccgg tattcgctag
    12481 tctggcaaac aaccctaaat ctgcctcacg ggtctgacgc agcataaact cacgtagcgg
    12541 ctgcgcccct ttttccagcg cctcctgcat tgatattttc tcttcgctga atggctggta
    12601 cgcatcgaca taaattttgt cgatcaccga tgacataata aaaaaggtca aaaatagtgc
    12661 cagccccagc aatacctggt taggtggcgc ggagggcgtt cccagcgcgt tacgcaataa
    12721 accaaaaaca atgatgatgc gggtgaaact ggtcatcatc agtaaaattg ccggaataaa
    12781 cgtcaacgag gtgatgaaca ccagcgtctg caccgggagc gaccagcttt gtccaccgcc
    12841 aggcagcggc tggctggtga tacccggcag ttgcgcgaag gcgaggggcg taaccagcca
    12901 gagaaggaca ggtgcgacag acaacaaacg acgcatcagg atctcccact acgcttaagc
    12961 aaatttttca tgaccgactg aaaatcggtc tgcggtatct cttctgttgg tgctgaaggg
    13021 ggaagtttat gcagcagatt gatttgccct gcggtaacgc cgagcaccag ccgtgcatct
    13081 tccacatcga ccaccacaac ccgttcacgc gcgcccagcg aagcactggc gctaattttc
    13141 agaccgttaa cgccagtgcg tttcggggca aatcccaacc gttttaccag ccaggcagca
    13201 gcgaggatca gggcaataat ggcgatgagt gcgccgctga cctgcagcag tggcgcagca
    13261 gaaaccggcg tggaagattg cacagtagcg tggttattca tcactaacgg ctcaggcgac
    13321 gcattcgctc agacggagta atgatatcgg tgatccgcac gccatattta tcggcaacga
    13381 ccaccacttc gccctgggcg attaaataac cgttgatcag aatatccagt ggttcgcccg
    13441 ccagaccgtc cagcgccacg acggaccctt gcgtcagacg caacagctct ttgatggtca
    13501 tccgcgtacg gcccagctcg acggtcagct tgaccggaat atccattatc aggtcgatat
    13561 cctgcaacgt tccgctgaca tcaccaccgc caaattgctg gaacaccgcg tcggcagcgc
    13621 ttttgctgct ggttgatttt tgttcgctca acgcttcagc ccacagatcg tccattgcgc
    13681 cgttgttgtc atcggccgga ttattcatgt cactcatttg ggctgttcct cgttcagaga
    13741 atttaaaatc gggttaatca aatgttctat ccgtaacgca tactgaccgt tgagagtgcc
    13801 gtactgactg gtcagcaccg ggacgccgtc aacatgggcg atgatgccat cgggtttttc
    13861 tatcggcagg acgtcgccgg gtttcagttt taaaatctgc gacaggcgta gcgagatatc
    13921 ggcaaagttg gcgaccagct ccagctgtga atgctgcacc tggcgcacca ggttatcgcg
    13981 ccagttctga tcttcattac gcgagttttc cagcggcggg ttaaccaaca attcccgtag
    14041 cggctcgatc atgctgaatg gcagacagat attaaattcg ccggtcaggt tgccaatctc
    14101 cacatggaac ggcgtgttaa ccacaatgtc gttcggcgag gtggtgatat tggtaaattt
    14161 cacctgcatt tccgaacgca cgtactcaac ttccagcgga ttaatcgcct tccaggcgtc
    14221 gctatagcct tcaagcgcca gctttaacat gcggttgatg acgcgctgtt cggtatgggt
    14281 aaactcgcga ccttccactt tggtcgggaa gcgtccatcg ccgccaaaca ggttatccac
    14341 ggcgataaac accagactcg gtgaaaacac caccagccct gtgccgcgca gcggtttcag
    14401 atggataagg ttcaggttgg tcggcaccgg caggttgcgg gcaaattcat ggtacggctg
    14461 aatgcggatg gccccgacgg ttatatccgg gctacgacgc agcaggttga acagccccat
    14521 acgaaaatgg cgggcaaagc gctcattaat gatttccagc gcctgcaaac gttcgcgcac
    14581 aacccgtcgt tgggtattcg gatcgtacgg acgaatgtca ctttcgccgc taacactggc
    14641 tgtcggttcg tctttgactt cgctgtcacc attcaacagc gcatcaattt cagcttgaga
    14701 aagaatacta tcgcccatgt cgttatcgca gaataaaagc ggtatacagc acgtcggtga
    14761 catcctgttt cggttgcccg gcaacaagcg gggtggaaag cgtggtttta atctcggcaa
    14821 tcaggttttt cttgccttct tctgtcgcca gtacggcagc atcctgacgc gaaaacagta
    14881 acagcaaacg gctacggact tccggcaaat actcactcag ccgcgagcgg gtagcttcat
    14941 ctttgagtcg cagggttata ccgatataaa gcacgcgatc cgcatcgccc aaattgaccg
    15001 taaaggtatc cagcgcatag aagaccggtg agggcacgac gcgatgctgc gctttgtctt
    15061 cagcggcaac ctgatgcgaa tgccagtagc tgtaacctgc gctggcacag gccgcgaggg
    15121 taatgaatac cagaatcggg agccaaagcg atcgcttgct tttcttgctt atcgcgtaat
    15181 cagtcatgtg ttgcggtctt cctgtgtcgc tactgcttat cggctaatta tcctgcgtct
    15241 tgagcgcaac aaagcgggga aaagacgcgg attacggtgc tacctctgac gttaggcgaa
    15301 aatatcaacg ccgctgttgc ctgttacgcg cccttgtaaa gagacgggaa ccggaagcgt
    15361 atcgtcttct tcccccgcca gaggttcatg gtttactgtg cgttggcttt gctgttgttg
    15421 ggaagcggcc tgctgctgac cactaaagct ttcgccactg atgttgcttt gccctaactg
    15481 aatgccactc tcggccagct gcgtacgcag taccggcagc gctgcttcca gggcggcgcg
    15541 tacgtgctga tgcggcgaaa ccatctggat ttgcgcctgg ttatcatcca ctttgaggga
    15601 gatttgcact tcacctaaat cctgcgggtg cagacgcaac tctgcacttt gttgtccctg
    15661 gcgggtgaac agcgaaatat gctggcttaa tgattgttgc cattcgtgag aacccagcgg
    15721 tgcactcaag acaggcgcgg cgacggtgag cagtggctgt gtctggtgtg gagtgattag
    15781 cgggctggcg gcagcggtca ccggtgaagg tgtgctgatg acttccgctt tactctgggc
    15841 ttctgctacc agcggtgtta atggctgagc tggcgtgccg ggggcatcat caggctgtgc
    15901 tgttgtgagt tgcgcagaag tcagttttgt gaagagcgtt ggtttctctg ccggtaacac
    15961 ggttgacggc gcatcagtca ctttgggcgt attgtcaaaa cccggcaaca tcgcaaaaag
    16021 ggcgctcagg cttgcggtga ggtcttcatt cagatcatcc gctttttcgt cttttgtcgt
    16081 atttttgtca gccaccgcag ccaacgtcat cgtatgagcg gtggttaacg gtgtcgatgt
    16141 ggattgttcg acgttgatga caggcagtgt ttcatccaca gggatcagta aatccgcttg
    16201 ttgcgcgtcg gaaacaatat cgctgatcag cagctcgcct tttgtcgtgg gcttatctgt
    16261 tgccaccagc acctgggggg ccgctttgtc ggtagttgtc tcgcttgcta atgcttcgct
    16321 caacaacgcg agaaaatctt gtgcagcatc gctggctttg ccgccaggca atgtggtggt
    16381 gtcaacgtcg gcggtaatca agggcgctaa gcgaatcatt caggtttcct catggcggcg
    16441 cgctgggcga actcatccat ctttttctga tcgaggcggt tttctgcaag cagtgccgcc
    16501 gtggattgcc gttcctgcag tgtctgccag gcctgcaaac gttgtttctt ttctcgccag
    16561 ctgttcaggg caatgtcaac tttctgcgtc cactgattaa gttgctggcg atgctgagta
    16621 atggcttttt ccagcgtctg gataaactgc tgatagttga tccagcggtt gctggtcatc
    16681 ccggcactca tatcgctgtt gaggttattg cgatattcat tctgataatc gatcagcatt
    16741 ttgagctgtt cttccgcctg ctgacacccg cgacgcattt cacccagcag gcgcgcggca
    16801 tcctctacct ctttttctgc cagatctttc agggtcgcca gcgcaccatg ttctgccatc
    16861 taccgttatc tcctgggtta tgacactgtc gggaaaatac gctccagccc ctggagagac
    16921 gcttcccagt ccgcacgttc aaaaatgcct tgttgtaaat agccctccag ctgcggccac
    16981 agggcgatgg ctttatcgag catcggatcg ctgcctttgg catacgcgcc gacgctaacc
    17041 agatcgcggt tacgctgaaa actcgacaac agctgtttga aggtgcgcac tcgcgcgtaa
    17101 tgttgctcac tgatcaacgc cgtcattgcg cggctgatcg acgcttcaat atcgatagcc
    17161 ggatagtgcc cggcttccgc cagtcggcga gacagcacaa tgtgaccgtc gaggatcgcc
    17221 cgtgcggagt ctgcaatggg gtcctgctgg tcatcgcctt cagtaagcac ggtataaaac
    17281 gcggtaatcg agccgccgcc gctaatgcca tttccggcac gctcgaccag tgccggtaat
    17341 ttggcaaaca ccgacggtgg ataacctttg gtggcgggtg gttcgccaat cgccagcgca
    17401 atttcacgct gggccatcgc gtagcgggtg agggagtcca taatcagcaa cacatgctga
    17461 ccacgatcgc gaaaatcttc ggcaatgcgc gtggcatagg cggcaccttg cattcgcaga
    17521 agcggagaga catccgccgg agcggcaatc accacggagc gtgcacgccc ttcggcaccg
    17581 aggatattct caataaaatc ttttacttcg cgcccacgtt caccaatcaa acccacgaca
    17641 atgacatcgg cgcgggtgta acgtgccatc atccccagca gcacactttt accaacgccg
    17701 gacccggcaa acagccccat acgctgccca cgcccaacgg taagcagggc attgattggg
    17761 cgcacgccgg tgtccagcac atgttcaatc ggtgtacgtt gcaacgggtt aaatggcggg
    17821 gtaatcagcg caccggtttc cgtcgtatcg ggggagggca ggccatcgag cggcttaccg
    17881 ctgccgtcca gaacgcgacc taataacgcc ggaccgagcg gcaactgctt gccgctttgc
    17941 agtccttcag ccgaaatgtt tttggcataa acccgcgcgc cgggcaggac accttccact
    18001 tcctccagcg gcattaaaaa cagccgttga ccgttaaagc caacgacttc gctttctact
    18061 tcgtgcgttt cgctgccgtt ctggcgttca atgacacagg ttgcgccgag cggcaattgt
    18121 aatccggtgg cttccagcac cagcccggta gcgcgggtta atcgcccata gcgacgtacc
    18181 gcaggcaact gcgccatttt ggcttcaaag ttatccagcg tggttagcca gcgagtcagg
    18241 cgcgtggtca ttacaccact cctggtgctg ccagacggca gagttcttgc cagcgagtgg
    18301 cgacactggc gtcgagatcg ccttcatcgg cggagacttt acagccgcca ggatggagtg
    18361 tgggatcgcc ccgcaagcgc cagccatgca aacttaaggt tgcgccgagc atatcatcca
    18421 cacgttgcag atcatccggg tgcacgcgca gctgtggttt accgctgaat aacggttctt
    18481 gctgcaacaa ctgttggatc tgtttgatca gtgccgagtt atccactgtt ggcgtctgac
    18541 cgatcacctg acgtgccgcc tccagcgcca tctgcatcag gcgcgaagca atcacactat
    18601 caagcgcatc aagggtagtt tgaaattcgc tgaccagttg ctgcatccgg gcatgaattg
    18661 gcgcttgttg agacttcgcc tctgccagac cttgctccag cccctgggcc agtccttcct
    18721 gatagccctg ctcatgacct tgctggcgac cttcggcaat ccccgcctga taaccttgct
    18781 catgggcctg catttgcagt tgcgccagtt gctgctcaag gctaggttcg gcctcttcaa
    18841 tgatggtttc ttccggctcg accatgggca caaactctgc ctgtggtggc gcgagatcgt
    18901 ccggcgtcca ggttttccac ggcagattat cagacatagg tatcctcgcc gctgccaatt
    18961 accatctcgc cagtttcggc aaggcggcgc acaatcagca gaatcgcttt ctgttcgttt
    19021 tccacctgcg acagacgcac cggaccacgg ttggcgagat cgtcgcgcag aatatcggcg
    19081 gcacgctgcg acatattgcg caggaatttc tcgcgcagtg gctgctcggc ccctttcagc
    19141 gcgatcaaca gcgattcgga atccacttcc tgcaacagac gctgaatgct gcgatcgtcg
    19201 acatccacca gattttcgaa caggaacatc tcgtcgataa ttttttgcgc cagctcgccg
    19261 tcgaattcgc gcacggcggt aataacggct tcttcctgct gagttttcat caggttgata
    19321 atttcggctg ccgttctcac gccgcccatt ttgctgcgct tgagattctg accgtcgagc
    19381 aagccattca gtacttcggt cagttccgcc agcgcggctg gctgcacgcc gccaaaggtg
    19441 gcgatacgca acatcacgtc gtggcgcaga cgttcatcga acaacgccag aatatcggcg
    19501 gcctgcgcgc gcttcagatg caccagaatg gtggcgataa tttgcggatg ctcatcgcga
    19561 atcagatcgg cggcactctg cggctccata aagttgagcg tttcaatacc gctggcggta
    19621 tcgcgagttt cgagaatatc ttccagcagg ctggcggcac gttcctcacc cagagctttg
    19681 accaataccg aacgcagata atcgttggcg ttgatattca gtgcggcaaa ctgttcagct
    19741 tcttgctcaa actccgccag cacataggtt agctgcttgt tggagatctg cgtgacgttc
    19801 gccattgcag cgctcagggt ttgcacttcg cgctgggaga ggtgcttgaa cacctctgcc
    19861 gcccggtctt cgccaatggt catcagcagg atgacgcttt tatcggtgcc tgtcaggtta
    19921 ctcatgatcg ttatttatcc actggcgaat gaccagcgcc accacgcgcg gatcgttatc
    19981 agacatttca cggatacgct ggctcatgac ttctgccccc agacgttggt tagcgcgtcg
    20041 ttgttggagt tgttcgtctt tgctcaggcg gacttccacc gcatcttcca cttcctcacg
    20101 ggcctgcgcc tgttgctgta cagctttcat cgcctcggcg cgacgtgtta actgcgggcg
    20161 taccgctttc cgccacagca gccacgccac cagcagtacc agcaaccagc gaccggcagc
    20221 cagcaactga tcgataaacg cttgctgttg ccagaacggc agttctccgc cgctttcgtc
    20281 actgctattg aacggcgagt taacgacatt gagcgagtca ccgcgttttt cagaaaagcc
    20341 catcgcctcg cgggtcagat cttcaatttg cttcatctgt tcgttgctga gaggcaatgg
    20401 tttaccgtct ggcaaggttt tgtaattcac cactaccgcg actgacagac gttgcacatc
    20461 gcccacgttc attttggtat gacgaatggt gcgatcgact tcgtagttac tggtttcatt
    20521 ccgctgtgtg ctacgcggcc cgctattgct ggtggtgctc gcctgctgct ggcggttatt
    20581 ttgatttgcc ggaggcgtgc tgattggcgc gttattcgca ggtgccggtt gattcgacaa
    20641 cgcccccggt acgccgcccg gataaccgga accgctttgc tcgctctcat taagctggcg
    20701 tgaacgaagc gccgcatgag attcatcacc gttagggcga tactgttctt ccgtttgttc
    20761 tttactggcg aagtccagtt gcgccgtgac ctgggcgtga atattaccgt taccaacaat
    20821 aggcgacagg attgcttcaa tacgccgctg aatacggcct tcgacatcgc tggcatattt
    20881 caactgagcg tcattaagat cgcgcccgct ggtattggac tgggttaaca gatgtccgcc
    20941 ctgatccacc agcgtgacgt ttcccggcgg cagaccagca acggcgctgg aaaccaggtg
    21001 cacaatggcg ctaatttgcc cttcatcgag tgcgcggccg ggtaacagat ttaccgtcac
    21061 cgatgcagaa ggggattttt gttcacggac gaataaagac ggtttcggca ttgccagatg
    21121 tacgcgcgcc ccttttaccg ggccgatagt ttcgatggta cgagaaagct cgccttccag
    21181 cgcccgctga taattcacct gttcgctgaa ctggctgata ccaaactttt cctgatcaag
    21241 cagttcgaaa ccgaccgcgc cgcctttggg caaaccttgt tgtgccaggc gcagacgcag
    21301 ttcgtgaact ttatctgccg gaacttcaat agcgccgctg gcttcgctga agcagtaagg
    21361 aatattcatt tgcgtcagtt ggctgacaat tgcgccacca tcctgatcgg aaagattgct
    21421 gaataatgtg cggtagtcgg gggctttcgc ccacaggatc agtgcgacca tgaccgccac
    21481 tgccgcggaa ccggcaacaa tcaatggaat tttcggattc gcacgcaggc gattaagcca
    21541 ctcaagagat tttgtctggg ctgcagtcgc attcatcgcg cacctcgtgg ctgattatgg
    21601 acggcgttat tggcaaagtg gaacaagact cactcccggg tcagcaaact cgaaaaattg
    21661 acgctgccat tatttgtgct ttcgtttttt tctatccgcc aataaacccg ttttttttgt
    21721 tgctatttag cgcctttgtc ttattgactt actggtaagc tttgctacca gaaattatcc
    21781 gggagatgaa aatgtcagcg atacagggga ttgaaggggt tatcagccag ttacaggcta
    21841 cggcgatgag tgcgcgtgcg caggaatcac tgccacaacc gaccattagt tttgccgggc
    21901 agctgcacgc cgcgctcgat cgcataagcg atacacaaac agccgcccgc acgcaggcag
    21961 aaaaattcac tctcggtgaa cccggcgtgg cgttaaacga tgtgatgacc gatatgcaaa
    22021 aagcctcagt ttctatgcaa atggggattc aggtgcgtaa taagctggtg gcggcttatc
    22081 aggaagtgat gtccatgcag gtgtaggttt tgtaacctgt tgttaattac atccgatctc
    22141 accgcgtggg gcatggatgg ggcaaactca ctcaatttct ggtttaaaat ggatacctga
    22201 tcctgaaagt tttctgccat ccacgatccg taaacgcgat aacatgtttt gtcttcgctt
    22261 tgctcatagg aagagtgaac tcttttgtta gcctggggtt ccttctgatc attattgttc
    22321 ctgcctttat ctcttgccac gccagagccc cctggataca catacaccag gatgaaaacg
    22381 gtgagttgtg ttcaaactgt agtacaattc tctccagtat gaacaggaaa gaatatgcta
    22441 tgaaccctta tatttatctt ggtggcgcaa tacttgcaga ggtcattggt acaaccttaa
    22501 tgaagttttc agaggggttt acacggttat ggccctctgt tggcacaatt atttgctatt
    22561 gtgcatcatt ttggttatta gctcagacac tggcttatat acctacaggg attgcttatg
    22621 ctatctggtc aggagtcggt atcgttctga ttagtttgct ttcctgggga ttttttggcc
    22681 aacgactgga cctgccagcc atcataggca tgatgttgat ttgtgccggt gtgttagtca
    22741 ttaacttgtt gtcacgaagc gcaccgcatt aaaaaataat catccttgag ataacaggaa
    22801 tctgatttgt gcatattatg gctaatgttc ggggcaactt tataattatg attgcatttg
    22861 cgcgatcatt gcgccttttt gcataatgag atcaatgcac atcaaataaa ataacgatag
    22921 gtcacaacaa agaggttttt atgaaaaaac ttatcgtttc aagtgtattg gcattcataa
    22981 cattttctgc gcaggccgca gcatttcagg tcactagtaa tgaaataaaa acaggagagc
    23041 aacttacaac gtctcatgta ttttctggag ttgggtgtga ggggggtaat acatcgccct
    23101 cattaacctg gtctggtgct ccagaaggca ccaaaagctt tgccgtaact gtgtatgatc
    23161 cagatgcgcc tacaggcagc ggatggtggc actggactgt tgctaatatt ccagcaacgg
    23221 taacatattt gcccgctgat gcaggaagac gtgatggtac aaaacttccg actggtgctg
    23281 tccaaggccg aaatgatttt ggctatgcgg gatttggtgg agcatgtcct cccaaaggag
    23341 ataaacccca tcattaccag tttaaaatat gggctctaaa aactgataag attcctgtgg
    23401 attctaattc cagcggagcg ttagttggtt atatgcttaa tgccaataaa attgcaaccg
    23461 ctgagataat acccgtttat gaggtaaagt aggataagag tatgctggca aaagatagga
    23521 ctaacctaaa gattgaagaa atacggatgc ataaacatca tgagattcac agggttaagc
    23581 ctcttatgcc agctttatgt cgtatccgtc agggaaaaaa agttatcaat tgggagacgc
    23641 atagtctaac tgttgataat aatcagataa tattatttcc ctgtggatat gaattttata
    23701 ttgcgaatta cccagaagca ggtctttatc ttgcagaaat gctttattat cccattgatc
    23761 taattgaaaa gttccaaaat ctttatgcga taaccgatca aattcgtaac acgacaggtt
    23821 tctgcttacc tcagaattcc gagttaatat attgttggga gcaactaaaa acatctattt
    23881 cccgaggctt ctcaacgcaa attcaggaac acttagcaat gggcgttcta ctttcattag
    23941 gagcacatca tgttaattgt ttacttttat cagatagtaa acaatcatta ataagtcgtt
    24001 gttataacct aatgctatcc gaacctggaa caaaatggac agcaaacaag gtagcgagat
    24061 atctctacat ttctgtttcc acattgcatc gccgtctggc aagcgaggga gtaagttttc
    24121 aaagtatatt ggacgacgtg aggttaaata atgcgttgtc tgctatacaa acgacagtaa
    24181 aacccatcag cgagattgcc agggaaaatg gttacaagtg tccttctcgt tttactgaaa
    24241 ggttccataa tcgttttaag ataacaccaa gagagctaag aaaagcgtcc agagagtaaa
    24301 agtattttat gaaggagcaa cagcatcgat ttttattttg gaaaataaat acggcatact
    24361 caacctattg gagcctgaac aatccggtga cttctgcgct aatcggggac gtttataagc
    24421 acatataatt gatactatct tcctccatca caatttcaga aatgcacgtg tgattttttt
    24481 gcatccggcg tttggactat gtggagttca agagtgggaa agttacaaga ggttgagggg
    24541 ataattaaag ggatgcttat gacattagat atccggatag gtacggatat cgttgttatt
    24601 aaatctagtt cgttattatt aaatctagtt tatacgttaa gatgcagcta cagcagaaat
    24661 tgccactgtt aattttttca tcgtgagccc ttttatttga actattattt aaaaatgatg
    24721 tcactgcact acaaatattc atccaatcaa tgagattatt tcaagatgta agttttagtt
    24781 tctcatttaa attgtgaagt agat
//
LOCUS       DAKISA010000059        24784 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.059, whole genome shotgun sequence.
ACCESSION   DAKISA010000059 DAKISA010000000
VERSION     DAKISA010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24784
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.059"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..1126)
                     /gene="fsr"
                     /locus_tag="OVW45_002492"
     CDS             complement(<1..1126)
                     /gene="fsr"
                     /locus_tag="OVW45_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCV5526914.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIAD"
     gene            1344..2996
                     /gene="ushA"
                     /locus_tag="OVW45_002493"
     CDS             1344..2996
                     /gene="ushA"
                     /locus_tag="OVW45_002493"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCV5526915.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMIGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(3033..3512)
                     /gene="ybaK"
                     /locus_tag="OVW45_002494"
     CDS             complement(3033..3512)
                     /gene="ybaK"
                     /locus_tag="OVW45_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCV5526916.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3716..4510)
                     /locus_tag="OVW45_002495"
     CDS             complement(3716..4510)
                     /locus_tag="OVW45_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCV5526917.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4648..4989
                     /locus_tag="OVW45_002496"
     CDS             4648..4989
                     /locus_tag="OVW45_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCV5526918.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(5204..7708)
                     /gene="copA"
                     /locus_tag="OVW45_002497"
     CDS             complement(5204..7708)
                     /gene="copA"
                     /locus_tag="OVW45_002497"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCV5526919.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTAEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKTGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADAGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            7970..8902
                     /gene="glsA"
                     /locus_tag="OVW45_002498"
     CDS             7970..8902
                     /gene="glsA"
                     /locus_tag="OVW45_002498"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCV5526920.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            8905..10197
                     /locus_tag="OVW45_002499"
     CDS             8905..10197
                     /locus_tag="OVW45_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCV5526921.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            10322..10729
                     /gene="cueR"
                     /locus_tag="OVW45_002500"
     CDS             10322..10729
                     /gene="cueR"
                     /locus_tag="OVW45_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCV5526922.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10730..11188)
                     /locus_tag="OVW45_002501"
     CDS             complement(10730..11188)
                     /locus_tag="OVW45_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCV5526923.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(11185..12102)
                     /locus_tag="OVW45_002502"
     CDS             complement(11185..12102)
                     /locus_tag="OVW45_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCV5526924.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGTSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            12248..12925
                     /gene="fetA"
                     /locus_tag="OVW45_002503"
     CDS             12248..12925
                     /gene="fetA"
                     /locus_tag="OVW45_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCV5526925.1"
                     /translation="MQENSPLLQLQNVRYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            12912..13691
                     /gene="fetB"
                     /locus_tag="OVW45_002504"
     CDS             12912..13691
                     /gene="fetB"
                     /locus_tag="OVW45_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCV5526926.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13754..14608)
                     /gene="cnoX"
                     /locus_tag="OVW45_002505"
     CDS             complement(13754..14608)
                     /gene="cnoX"
                     /locus_tag="OVW45_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCV5526927.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNFTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGMVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14669..15478)
                     /gene="ybbO"
                     /locus_tag="OVW45_002506"
     CDS             complement(14669..15478)
                     /gene="ybbO"
                     /locus_tag="OVW45_002506"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCV5526928.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(15468..16091)
                     /gene="tesA"
                     /locus_tag="OVW45_002507"
     CDS             complement(15468..16091)
                     /gene="tesA"
                     /locus_tag="OVW45_002507"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HCV5526929.1"
                     /translation="MNFNNVFRWHLPFLFLALLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            16062..16748
                     /locus_tag="OVW45_002508"
     CDS             16062..16748
                     /locus_tag="OVW45_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCV5526930.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16745..19159
                     /locus_tag="OVW45_002509"
     CDS             16745..19159
                     /locus_tag="OVW45_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCV5526931.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYDKWLLPQLK
                     PEQRWYGLEQDEDALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMQLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(19393..20487)
                     /gene="mnmH"
                     /locus_tag="OVW45_002510"
     CDS             complement(19393..20487)
                     /gene="mnmH"
                     /locus_tag="OVW45_002510"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCV5526932.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQAAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTDSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTQ"
     gene            complement(20556..21482)
                     /gene="allS"
                     /locus_tag="OVW45_002511"
     CDS             complement(20556..21482)
                     /gene="allS"
                     /locus_tag="OVW45_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCV5526933.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            21712..22194
                     /gene="allA"
                     /locus_tag="OVW45_002512"
     CDS             21712..22194
                     /gene="allA"
                     /locus_tag="OVW45_002512"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCV5526934.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            22272..23087
                     /gene="allR"
                     /locus_tag="OVW45_002513"
     CDS             22272..23087
                     /gene="allR"
                     /locus_tag="OVW45_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCV5526935.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            23177..>24784
                     /gene="gcl"
                     /locus_tag="OVW45_002514"
     CDS             23177..>24784
                     /gene="gcl"
                     /locus_tag="OVW45_002514"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCV5526936.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVAECQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGCDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERKVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFE"
BASE COUNT         5734 a         6431 c         6699 g         5920 t
ORIGIN      
        1 gatcggcgat aagccccaga actgccgctc ccagacctcc catgccaaaa gcaaaaccga
       61 aaaagagtcc agaaaccata ccgatacgtc ccggaagtag ctcctgagcg tagaccagaa
      121 tggcagagaa tgccgaagcg aggataaatc caataatcac cgttaaaacc cccgtccagt
      181 gcaggctggc gtagggtaaa atcagcgtaa acggcgcaac gccgaggata gagccccaaa
      241 tcacatattt ccgtccaatt ttatccccta caggcccgcc gatcaccgta cctgccgcaa
      301 cggcaaacag gaaggcaaac agatgaagct gggcattctg gatagataat ccgaattttt
      361 gcatcagata aaaggtgtaa tagctgctga tgctcgccat atagaaatat ttcgagaaaa
      421 tgaggattaa cagaatgctg actgccagta ccactttgtt gcgcggcagt ggattgatta
      481 tcgtcgcttt gggttttcct ttattcattc ggtgctgtgc cgagtaccaa cggctaattt
      541 gcgccaacac cacgatcgcc agcagtgccg caagcacaaa ccaggcaacg ttgcctttgc
      601 cataaggcgc gataatcacc gccgccagca agggtcccag ggaactgcca aagttgccgc
      661 cgacctgaaa gatagattgc gccaggccat gccgcccgcc ggaagccata cgggctacgc
      721 gagaagattc cggatgaaag accgatgaac cggtaccgac cagcgccgcc gccagcagaa
      781 ctgcgccaaa actgcccgcc agcgcaagca gcaccagacc acttaaggta aagcacatgc
      841 caattggcaa cgaccacggc atcggatatt tatcggtcca gtagccgacc actggttgca
      901 gtagcgaaga ggcgagctgg aaggtgaggg ttatcatgcc aatctgcata aatgtcagag
      961 aaaattctga ctgaagcagc ggataaatcg ccagaatcag cgattggatc atgtcgttca
     1021 gcagatgtga gaggctgata gcacctaaaa taccaaacga tgttcgggcc ttggtcgttg
     1081 acgcagccgc gcccgccaca ggctggggtt gttcactcat tgccatagga aagtcacttt
     1141 ttcagggttg cgatgtaaag aatgatctta tttgtgatta ttacccgact aacatacctg
     1201 tatgcgtcgt ctgaaggaag tctcaacgcc gaatacagaa tttctaatct ggatgcagat
     1261 ttatcttcac cggacgcaga cttgtctatg atgtcgcgtc atactatttt tcaacacgtt
     1321 gaaatcaggt cagggagaga agtatgaaat tattgcagcg gggcgtggcg ttagcgctgt
     1381 taaccacatt tacactggcg agtgaaactg ctctggcgta tgagcaggat aaaacctaca
     1441 aaattacagt tctgcatacc aatgatcatc atgggcattt ttggcgcaat gaatatggcg
     1501 aatatggtct ggcggcgcaa aaaacgctgg tggatggtat ccgcaaagag gttgcggctg
     1561 aaggcggtag cgtgctgcta ctttccggtg gcgacattaa cactggcgtg cccgagtctg
     1621 acttacagga tgccgaacct gattttcgcg gtatgaatct ggtgggctat gacgcgatgg
     1681 cgatcggtaa tcatgaattt gataatccgc tcaccgtatt acgccagcag gaaaagtggg
     1741 ccaagttccc gttgctttcc gcgaatatct accagaaaag tactggcgag cgcctgttta
     1801 aaccgtgggc gctgtttaag cgtcaggatc tgaaaattgc cgttattggg ctgacaaccg
     1861 atgacacagc aaaaattggt aacccggaat acttcactga tatcgaattt cgtaagcccg
     1921 ccgatgaagc gaagctggtg attcaggagc tgcaacagac agaaaagcca gacattatta
     1981 tcgcggcgac ccatatgggg cattacgata atggtgagca cggctctaac gcaccgggcg
     2041 atgtggagat ggcacgcgcg ctgcctgccg gatcgctggc gatgatcgtc ggtggtcact
     2101 cgcaagatcc ggtctgcatg gcggcagaaa acaaaaaaca ggtcgattac gtgccgggta
     2161 cgccatgcaa accagatcaa caaaacggca tctggattgt gcaggcgcat gagtggggca
     2221 aatacgtggg acgggctgat tttgagtttc gtaatggcga aatgaaaatg gttaactacc
     2281 agctgattcc ggtgaacctg aagaagaaag tgacctggga agacgggaaa agcgagcgcg
     2341 tgctgtacac ccctgaaatc gctgaaaacc agcaaatgat ctcgctgtta tcgccgttcc
     2401 agaacaaagg caaagcgcag ttggaagtga aaataggcga aaccaatggt cgtctggaag
     2461 gcgatcgtga caaagtgcgt tttgtacaga ccaatatggg gcggttgatt ctggcagccc
     2521 aaatggatcg cactggtgcc gactttgcgg tgatgagtgg aggaggaatt cgtgattcta
     2581 tcgaagcagg cgatatcagc tataaaaacg tactgaaagt gcagccattc ggcaatgtgg
     2641 tggtgtatgc cgacatgatc ggtaaagagg tgattgatta cctgaccgcc gtcgcacaaa
     2701 tgaagccaga ttcaggtgcc tacccgcaat ttgccaacgt tagctttgtg gcgaaagacg
     2761 gcaaactcaa cgacctgaaa atcaaaggcg aaccggtcga tccggcgaaa acttaccgta
     2821 tggcgacatt aaacttcaat gccaccggcg gtgatggcta tccgcgcctt gataacaaac
     2881 cgggctatgt gaataccggc tttattgatg ccgaagtgct gaaagcgtat atccagaaaa
     2941 gctcgccgct ggatgtgagt gtttatgaac cgaaaggtga ggtgagctgg cagtaatccg
     3001 aaagtgccgg atatttgcat ccggcacaat gcttaatcgc ggcgggcgat atcagcaaat
     3061 ttggcatcga ggatcttcgc cagatcgcct gccgccagtt cgatatccag tccgcgcttg
     3121 ccgccagaaa cataaatagt ggcaaattct tgtgcggggg cgtcgataat cgtcggcaga
     3181 cgttttttct gccccagtgg gctaatcccc ccaaccaggt atcccgtcga acgctgcgcg
     3241 gccatcggat cggccatctc aactttcttg gcacccagcg cttttgctac ttttttaaga
     3301 tccagttgac ctgcgaccgg cgtaacggcc actgccagat gtttcatatc accgttcact
     3361 gccaccagta gcgttttgta gacttgatcc ggattcaaac ctaatttttt gacgacttca
     3421 tcgccaaaat tggtttcagc cggatcgtgc tcgtaggtat ggatttgaaa cgaaatcttg
     3481 tttttttcga gtaatttaac tgcgggtgtc atggtaattt tcctgcctta aacaaaaaaa
     3541 cgaagcaagc atacgcctga tggcggtcca aaaaaaagag tcatcttgcc taagagtatt
     3601 ggcaggatgg tgagattgag cgacaatcga gttacaccgt cgcttaaagt gacggcataa
     3661 taataaaaaa atgaaattcc tctttgacgg gccaatagcg atattggcca tttttttagc
     3721 gcaacatttg cggcaaattc ccttctccat acaggtgtag tgcaccgacc gcgaccacat
     3781 atcgccccgg cggcatggcg cgtaatttat cccgccaggc gagatttcgc tgatgcatca
     3841 gcacatcgta cagcgactga ctgaacgtat tgggcagcgt tatatcatta ttttgcggag
     3901 gtgcattcag ccaccagctc atcatttgtt gcagcaaccg tgcgttggta tgccagtggg
     3961 tcagcgtatc gtccagcagc gccagtcctt tgtcagggag ctggagcaac atggtaatct
     4021 ggttttcagc cccttccagt tcaatcacgg gtttatgttg ttgcttcgcc gcctgcaata
     4081 actggtaatc gataccgtat tctgcccgca gccccagttt ttgtgcctgc gtcgcctgaa
     4141 gaaccatcgc gatttgccac agcggttgag tagaaaagag cgacggagaa atgcccatct
     4201 cctggctaat gtgctgcaga ttttggagtt gctcctcgtt aatgcgctct tccagcgcct
     4261 cgcaagtagg caaattagca aaaggcgtat cgctggtgga aacatccgcc tcgacgatca
     4321 gcgcatcggc gtttttgagc tttttgagca aacgggtggg caggggagcc atatcgtggc
     4381 tacccatatg aatactgcca atcagatgaa aatggcgatt gccgggaagg gtgatatcga
     4441 tggcaggcca ggtgtaatga ttaccgcgca gcgcggccca aagcgttttt acccggtaca
     4501 acagatccat acgacctccc tttgtgaaat atcatgctag cgcgcggtga gggatggcgc
     4561 aaatggagtt ggggacttgt ttaatgtgtt tgtatgattc agtatgttct tgcatcgcta
     4621 ttcacaggga agcaacagtt aaaaaccatg aaacaggcaa caagaaaacc gacgacaccc
     4681 ggagatattc ttctctatga atatctggaa ccgctcgatt tgaaaatcaa tgagttagca
     4741 gagttgctgc atgttcatcg taatagcgtc agtgcactga tcaataacaa tcgtaaactc
     4801 accactgaga tggcattccg tctggcgaaa gtttttgata ccacagtcga ttttaggcta
     4861 aacctccagg cggcagttga tctttgggaa gttgaaaaca acatgcgcac ccaggaagaa
     4921 ttgggacgga ttgaaacagt ggctgaatat ttggcacgcc gtgaagagcg tgcaaaaaag
     4981 gtcgcgtaag gccaaaacat cgcttcatct cgtaggccgg ataaggcgtt cacgccgcat
     5041 ccggcatccg agcaccattg cctgatgcga cgcttacccg tcttatcagg cctacaaacc
     5101 tgtgcctgaa ccgtaggccg gataaggcgt ttacgccgca tccggcagtc atgcgtcgat
     5161 gccaaatgcg ccgccctaaa gcagcgcatc cgcaatgacg tacttattcc ttcggtttaa
     5221 accgcagcaa ccggttggcg ttactcacta cggtaatcga cgagagcgcc attgccgctc
     5281 cggcaactac cgggttaagc agtgttcccg taaacggcca caaaataccg gcggcgaccg
     5341 gaataccgat gctgttgtag ataaaggcac cgagcaggtt ctgcttcatg ttgcgcagcg
     5401 ttgcgcggga aatcgcgagc gcatccgcaa cgcccatcag gctatggcgc atcagggtaa
     5461 tcgccgcggt ttcaatggca acatcactgc cgccacccat cgcaatgccg gcatccgcct
     5521 gggccagcgc tggcgcgtcg ttaatgccgt cgcccaccat tgccacctga cgtccttcac
     5581 tttgcagacg tttgatcgct tcggctttac cgtccggcag cactccggca atcacttcat
     5641 caattccagc ttctttggcg atcgcattgg cggtggttgg gttatccccg gtcaacatca
     5701 ccagacgata tcccgtttta tgcaggcgtt gcagcgccgc cacgctatca ctacgcaacg
     5761 gatcgcgtac agccagcagg gctactgctt tcccgtcaat cgccagcagc acaggcgttg
     5821 ctccttgcga tgcctgagca gtaatctccg cttcgatagc tttggtacca acctgttgct
     5881 catttaacag cgcctgattg cccagcaata acgcatgacc ttcagcttca ccgctcacgc
     5941 ccagcccgcg caatgtgcgg aaaccgttga cctgcggcag ctgcatatca cctgctttat
     6001 cgaggatcgc ccgtgccagc ggatggctgg aaccttgctc cagtgccgcc gccagacgca
     6061 atgcctgcgc ttcatcaaca tcagcaaacg ttttcactgc gacaacctgc ggcttccctt
     6121 cggtcagcgt cccggtttta tcgaacacga cggtatcgag tgtactggcg cgttgcagcg
     6181 cgtcggcgtc ccgcaccagc acgccaaact cagccgcccg cccgacgccg gaaataatcg
     6241 acatcggcgt cgccagcccc agcgcacacg gacaggcgat aatcagtacc gtggtggcaa
     6301 tcaccagggt ataaacaatt tgcggtgccg gaccaaagaa gtaccagatt gccgcactga
     6361 ccagcgcaat caccaccacc acaggtacaa atacggctga gattttatcc gccagctgac
     6421 cgatttctgg cttgctgctc tgggcctggc gcaccatgcg aatgatccgt gacagcgtgg
     6481 tatggctgcc aaccgcactg gcacgaaaca gcacactgcc gtcctgtacc actgtcccgg
     6541 catggacgct ttcgccttcg cctttttgct gtgggattgg ttcgcccgtc agcatcgctt
     6601 catccagcca tgcttcgccc tgggtaatct cgccatctac cggcacgcga tcgccggtcg
     6661 tcaggcgcag taacatccct ggctgaactt ccgccagcgg cacgcttttt tcaccctctg
     6721 cggtgaccac tcgcgccgtc ggtggggtta aatcgagtaa tttctccagc gccttagaag
     6781 aacgctgacg tgcgcgcgct tccagcatat ggccgagatt aatcagaccg ataatcatcg
     6841 cactggcttc atagtaaaga tgccgcgctt ccatcgggaa ccactgcggc cacaggttga
     6901 cgctcatcga atagagccac gccacacccg ttcccagcgc caccagcgta tccatcgtcg
     6961 ctgcaccgtt cagcaggctt ttccatgcac tgcggtaaaa atggccgccg gcgaaaacca
     7021 tcactgccag ggttatcagg ccgataacta accacaagct gcggttgtca gcggtgacca
     7081 tcatgttatc gccgatcatc ccccagacca ttaccgggat acccacagcc agtgcaacaa
     7141 ttgcctgcca gcggaagcgc ttcatcgtag ctacggcggt ttcttgctgg cgctcgcggc
     7201 gtttagcgtc atcttcaatc gcttccgcac cgtagcccgc tttttccacc gcctgcacta
     7261 aatcttgtgg ggaggcactg cccatcacca gcgcagtacg ctccgccagg tttacccgtg
     7321 cctgagtgac gcccggtacg ctttgcagcg cattttgtac gcgggtgaca cagctggcgc
     7381 agctcatgcc gctcagcagc aactgctggc tgtcatcgtc atcggcggtc gctgccggaa
     7441 gcgcctcaga aaccgctgtc agtgcttccg acgggattga tgactccgcc agcggtttag
     7501 cctttgggtg gcttacagat gcgtcataac ccgcttgttt gatggtttcg atcagctgtt
     7561 ctgcactggc agtcccggta acgtgcgctt cagtgataga cacatccgcc tgctcaacat
     7621 ccgggcgctg ttcaagactt tctttcacgc gtttaacgca gtgaccgcag gacaggccgt
     7681 ccagggtcag gtcgatagtt tgtgacataa aacactcctt taagacagtt ttgactggct
     7741 gtgataaagg ttaaaccttc cagcaagggg aaggtcaaga aattaataaa ccaggcgggt
     7801 aaaagtccgt aaagattaaa aaatcggctc gatttgcatc aggattagac atttatttct
     7861 ttgttttcct gtagttaagt tgcgggtgct aagttaaatc aggatgcctg aaaatcggca
     7921 ccggggtgag gaattacctc ccgcatctat aaaaaggagt taacaaaaga tgttagatgc
     7981 aaacaaatta cagcaggcag tggatcaggc ttacacccaa tttcactcac ttaacggcgg
     8041 acaaaatgcc gattacattc cctttctggc gaatgtacca ggtcaactgg cggcagtggc
     8101 tatcgtgacc tgcgatggca acgtctatag tgcgggtgac agtgattacc gctttgcact
     8161 ggaatccatc tctaaagtct gtacgttagc ccttgcgtta gaagatgtcg gcccgcaggc
     8221 ggtacaggac aaaattggcg ctgacccgac cggattgccc tttaactcag ttatcgcctt
     8281 agagttgcat ggcggcaaac cgctgtcgcc actggtaaat gctggcgcta ttgccaccac
     8341 cagcctgatt aacgctgaaa atgttgaaca acgctggcag cgaattttac atatccaaca
     8401 gcaactggct ggtgagcagg tagcgctctc tgacgaagtc aaccagtcgg aacaaacaac
     8461 caacttccat aaccgggcca ttgcctggct gctgtactcc gccggatatc tctattgtga
     8521 tgcaatggaa gcctgtgacg tgtacacccg tcagtgctct acgctcatca atactgttga
     8581 actggcaacg cttggcgcga cgctggcggc gggtggtgtg aatccgttga cgcataaacg
     8641 cgttcttcag gccgacaacg tgccgtacat tctggccgaa atgatgatgg aagggctgta
     8701 tggtcgctcc ggtgactggg cgtatcgcgt tggtttaccg ggcaaaagcg gtgtaggtgg
     8761 cggtattctg gcggtcgtcc ctggcgtgat gggaattgcc gcgttctcac caccgctgga
     8821 cgaagaaggc aacagtgttc gcggtcaaaa aatggtggca tcggtcgcta agcaactcgg
     8881 ctataacgtg tttaagggct gatcatgatg aacacggaag gtaataacgg taacaaaccc
     8941 ctcggtctat ggaacgtcgt ttccatcggt attggggcaa tggtgggggc ggggatcttc
     9001 gcgctgctgg ggcaggctgc gttgctaatg gaagcctcga cctgggtcgc ctttgctttt
     9061 ggcggtattg tggcgatgtt ttccggttat gcctatgcgc gtctgggggc gagctatccc
     9121 agcaatggcg gtattatcga cttttttcgt cgcggattag gcaacggcgt cttttcgcta
     9181 gcgctctcgt tgctgtacct gttgacgctg gcggtgagca tcgccatggt tgcccgtgct
     9241 tttggcgctt atgccgtgca gtttttgcat gaaggcagcc aggaggagca ccttattttg
     9301 ctctacgcgt tggggatcat tgcggtgatg acgcttttca actccttaag caaccatgcg
     9361 gtagggcggc tggaagtgat cctcgtcggc attaaaatga tgatcctgtt attgctgatt
     9421 attgccggtg tctggtcgct gcaaccggcg catatttccg tctctgcgcc ccccagctcc
     9481 ggtgcgttct tctcctgtat tgggataacc ttccttgcct atgcgggctt tggcatgatg
     9541 gcgaacgcgg cggataaagt gaaagatccg caggtcatta tgccacgggc gtttctggtg
     9601 gcgattggcg ttaccacgtt gctttatatc tcgctggcgc tggttttgct tagcgatgta
     9661 tcggcattag agttagaaaa atatgctgat accgccgtag cgcaggctgc ttctccgctg
     9721 ctcgggcatg tgggttatgt gatcgtcgtc atcggcgctt tactggcgac ggcttcagcc
     9781 attaacgcga acctgttcgc cgtgtttaac atcatggaca acatgggcag cgaacgcgaa
     9841 ctgccgaagc taatgaataa atccctgtgg cggcagagta cctggggcaa cattattgtc
     9901 gtggtgttga ttatgctgat gacggcggcg ctaaatttag gctcactcgc cagtgttgcc
     9961 agcgccacct ttttgatttg ctacctggcg gtgtttgtgg tggcgatccg cctgcgtcat
    10021 gatattcacg cctcgttgcc gattcttatc gttggtacgt tggtgatgtt gttggtgatc
    10081 gttggcttta tctacagtct gtggtcccag ggtagccgtg cgttgatatg gattattggc
    10141 tcactcttac tcagccttat tgtggcaatg gtcatgaagc gcaataaaac cgtataacat
    10201 ctctctgtgc gcagtacttc ctgtattatt gtggtggcgg tcgatattcg cactggcaaa
    10261 aaaacgtgct tgaatatctg ttgaaaccct ttaacaaagc acaggaggcg ttgcgcgaac
    10321 gatgaacatc agcgatgtag caaaaattac cggcctgacc agcaaagcca ttcgcttcta
    10381 tgaagagaag gggctggtga cgccgccgat gcgcagcgaa aacggttatc gcacctacac
    10441 gcagcagcat ctcaacgaac tgaccttact gcgccaggca cggcaggtgg gctttaacct
    10501 ggaagagagc ggcgagctgg tgaatctgtt taacgacccg cagcggcaca gcgccgacgt
    10561 caaacggcgc acgctggaga aggtggcgga gatcgaacga cacattgagg agctgcaatc
    10621 catgcgcgac cagctgctgg cactggcgaa tgcctgccct ggcgatgaca gcgccgactg
    10681 cccgattatc gaaaatctct ccggctgctg tcatcatcgg gcagggtgat taagacgaga
    10741 cggctcggat atgtagggtt atcccttcta tcgcaatgac ttcaacatgc gtacctgcac
    10801 cgagatcctc gctggcgctg acaggccatg aactgtcacc gacgcgcata tgaccgcgcc
    10861 cgttgaccag cggagattcc agcacaaaac gtcggccaat cagctgctgc ccgcgctggt
    10921 ttaaatgact atcgctgtgc ttttgttcgc gcacccgccg cgacaaccat ttccaccaca
    10981 gccaggcggc gagcagcgtc aggatggcaa acattacccc ttgccactcc caacccagcg
    11041 gcaccagcca gaccaccagg ccggtaatca ccgctgccac gccgctccac aacaaataac
    11101 catttccgcc cagcatctcg gctgccagca gcaaaccgcc gagactgagc cagaaaatat
    11161 gtggatgaac gaccattaac tccatcatgg ctgagtccgt ttgttggcgc tgtctttcac
    11221 cagctcggca atcccggcaa tcgaccccat caggctgctg gcctctaatg gcatcatcac
    11281 tactttgctg ttactggagg taccgatctg ctgtaacgct tcggtgtatt tctgcgctac
    11341 gaagtagttc accgcctgaa tatcaccgga ggcgatagct tcagacacca ttttggtggc
    11401 gcgggcttct gcttcggcgg aacgttcacg cgcttcagcc tgtaaaaacg ccgactgacg
    11461 ttcgccttcc gctttcagga tttgcgactg tttttcacct tcggctttga ggatttccgc
    11521 ctgacggatc ccttccgctt caagaatgta agcgcgtttg gtacgttccg ctttcatctg
    11581 cgcgttcatt gaagagataa gctctgccgg tgggcgcacg tcgcgaattt caatacgggt
    11641 gactttaatc ccccacgggt tggtggcttc atcgacaata cgcagcaggc gtgagttgat
    11701 gctgtcgcgc tgagagagca tttcgtcaag ttccattgaa cccaacacgg tacggatgtt
    11761 agtcatggtc aggttgatga tcgccagctc cagattgctg acttcataag ccgcgcgcgg
    11821 cgcgtcaatc acctgaataa aacagactgc gtcgatggta acgttggcgt tatctttcga
    11881 gataacttcc tgggaaggga tatcgagcac ttgctccatc atattgatct tgcgaccaat
    11941 gcgatccata aacggcacca ccagactgag ccccggctgt aacgttttgg tatagcgacc
    12001 aaaacgttct accgtccatt gatagccctg cggcacgatt ttgacgcccg cgccgacaat
    12061 gaccagcgcg acaaaaatga gaatcgggat aaagataagc atcggaaaaa cctcctgttg
    12121 taccgtccat aatcagcaaa attgctgctt gattaaacaa attatacctg attactgaaa
    12181 gagagttccc ccttattcct gcgaaggata aactgttttt agtaaaaatc agaaaaaggg
    12241 aacagcgatg caggaaaata gtcctttgct tcagctacaa aacgtaagat atctggcggg
    12301 tgatgcgaag attcttaata acatcaattt ttcgctgcgt gctggcgaat ttaagttaat
    12361 taccggtcct tctggttgtg gcaaaagtac gctgctaaaa atagttgctt cattgatcag
    12421 cccaaccagc ggaacgttac tgtttgaagg tgaggatgtc agcacactaa agccagaaat
    12481 ctaccgccaa caagtctctt actgcgccca gacaccgacg ctgtttggcg atacggtata
    12541 cgataatctg atctttccct ggcagatccg taaccggcag cctgacccag ccatttttct
    12601 cgattttctc gaacgcttcg ccttgccgga cagcattttg acgaaaaata tcgccgagct
    12661 atctggtggt gaaaaacaac gcatctcact gattcgtaac ctgcaattta tgccgaaggt
    12721 tttattgctg gatgaaatca ccagtgcgct ggatgaaagt aataaacata acgttaatga
    12781 gatgatccat cgttatgtgc gcgagcaaaa tattgccgtg ctgtgggtga cacacgataa
    12841 agacgaaatt aatcatgcgg ataaagtgat tacactgcaa ccgcatgccg gagaaatgca
    12901 ggaagcacgc tatgaactcg cataatatta ctaacgaatc attagcactg gcattaatgc
    12961 tggtggtggt ggcaatctta attagccata aagaaaaact ggcgctggag aaagatattc
    13021 tctggagcgt cggacgagcg ataattcagc tgattattgt cggctatgtg ctgaagtata
    13081 ttttcagcgt ggatgatgcc agcctgacat tattgatggt gttatttatc tgctttaatg
    13141 cggcgtggaa cgcgcaaaaa cgcagtaaat atattgctaa agcttttatc tcatcgttta
    13201 ttgccattac ggtcggggcg ggaattaccc tggcggtgct gattctctca gggtcgattg
    13261 aatttatccc gatgcaggtg atccctatcg ccgggatgat tgccggtaac gccatggtag
    13321 cggtggggtt gtgttacaac aatttagggc aacgggtcat tagcgaacag caacagattc
    13381 aggagaaact gagtcttggt gcgacgccga agcaggcttc agcgatattg attcgcgaca
    13441 gtattcgcgc ggctttaatt ccgacggtcg attcagcaaa aacggttggc ttagtgagtt
    13501 taccaggaat gatgtccggg ctgatatttg ccgggattga tccggtgaag gcgattaaat
    13561 atcagattat ggtgaccttt atgctgctct caaccgccag cttgtcgacc attattgcct
    13621 gctatttaac ctatcgtaag ttttataatt cgcgccacca gttggtggtg acgcaattga
    13681 agaagaaatg atgcgatgcc ggatgcgcag catcgcatcc ggcgttgtgg ttcatgtgcc
    13741 gatcaacgga atatcaatac aacaatgcat acagctggcg gcgatacttc gacgccagtg
    13801 catcacccgt acccagcgca gcgaggatct cctggaacgt tttacgcgtc tgaccgtctg
    13861 cggcggtgag atctttacgc agatgcccga acagcaactc cagcgcctct tcattgcgcc
    13921 caacctgatg cagttgcagc gccagttgcg tcgccagtgc ggcatcttct ggattctccg
    13981 ccacctgctg ttgcaactgt tgaatttccg gcgtatcagc cgcctgcttc agcagttcga
    14041 tttgcgccac catcccctgg tagcgggtgt cctgatcctg taacggaatg gttttcagca
    14101 ccgcttctgc atcttcagaa cggttcagcg caatcagcgt ttctgccaac agcaggccga
    14161 tctccccgtt ctgattcgac aactgccagg cgtctttcag caatggcagg gcatcggtga
    14221 aattgccttc ctgcatcagt tgcatcgcct gctgcgcttt cagctcttct tcgcgcggca
    14281 gcactttatc cagcagggcg cggatcgcct cttccggttg cggcccctgg aagccatcta
    14341 ccggttgccc gttctggaac agatacaccg tcggaatcgc gcgcagacca aactgggcgg
    14401 caatcatctg ctccgcatcg cagtccagct tcgccagaat aaattgcccg ttgtactgcg
    14461 ccgcaaggct ttccagaatt ggggttaact gcaaacagtg ctggctacgt tcagaccaaa
    14521 aatagaacag caccggagtg gtcatcgact gttcaagaac ctgttgcagg ttagattcgt
    14581 taatgttgac aatattttct acggacatgg agtcgctctc tgttgtcgat attttctttg
    14641 acatgggggc ttaagcgcgc gcttcaactc acccctgcaa tattttgtcc atcacgcgcc
    14701 ccggcagcag gcgcttaagc accattactg cccaagtcac cagcgttacc ggatagcgca
    14761 tcttcggctt ctcgctaata aaagcatggc gtactttgtc caccaccgct tccggtccca
    14821 acgtaaagcg ggcggcgatg ccgggatttt cgactggttt atcactttgc gtctggttga
    14881 cgttgtcggt gaagcgagta cgaatgggac cgggttcgat caggctgact ttaattccgc
    14941 tgtggcgtag ctccatgcgc agcgcgtctg accatgcctc cagcgcatat ttactggccg
    15001 cgtaagcgcc acgacccggt gtggagatta atcccatcac cgatgatgtc atcacaatac
    15061 gcccttcacc gtgcggtaac atcgcgggta acaggcgcat ggtgagctgg tgtgcgccga
    15121 aaaagttggc ggaaaactgc tgttccatct gcgcgcggct gatggtggaa agggggccat
    15181 acatgccgaa tccggcattg ttaaagatcc catacagaca attatcggtc agggcgatca
    15241 cttcgtcggc tgcgcgatca acactttctg gcgaatccag atcgatcaac acgccggtaa
    15301 atcccatgct gttcatacgc tcaacatcat ccggtttccg gcaacctgcc agcacatgaa
    15361 aaccctggcg ttttaattcg agcgcgcttt ccaggccaat tccactggaa catccggtaa
    15421 ttaagaccga tttttgcata actttacctg tcaggatctc cgttgcttta tgagtcatga
    15481 tttactaaag gctgcaactg cttcgccatc cagtcggcaa taaacggctg ggcgtcgcgg
    15541 ttgggatgaa taccgtcatc ctgcatccat tgtggcttga ggtagacctc ttccataaaa
    15601 aagggcagca gcggaacatc aaactctttg gcgagtttgg ggtaaatggc gctaaaggct
    15661 tcattataac ggcgaccata gtttgcaggc agacgtattt gcattaacaa tggttcagcg
    15721 ttggcggctt tgacatcctg caaaatctgg cgcagcgttt gctcggtttg ctgtggctga
    15781 aaaccacgca aaccgtcatt gccgcccagt tcaaccagca cccaacgcgg ctgatgctgt
    15841 ttcagcagag ccggaaggcg cgccagtcct tgttgcgagg tgtcgccgct gatgctggca
    15901 ttaactaccg acgttttact ctgccactta tcattcaaca aggcaggcca ggccgcgctg
    15961 gcagacattc gatacccggc gctcaggcta tcacccagaa tcaataacgt gtccgctgcg
    16021 gcggcacgga aggttaacag ggccaggaac aggaagggca aatgccagcg gaaaacattg
    16081 ttgaagttca tcatcttaag aagtccgtcg gtcaggggga gcatgaactc tccatcctca
    16141 ccggagttga gctggttgtc aaacgtggcg agaccatcgc actggtgggc gagtcgggat
    16201 cgggtaagtc aaccttgctg gcgatcctcg ccgggcttga tgacggcagc agtggcgaag
    16261 tgagtctggt gggacaaccg ctacataata tggacgaaga agcgcgggca aagttgcgcg
    16321 cgaagcacgt cggctttgtt tttcagtcat ttatgttaat tcctaccctt aacgcgctgg
    16381 aaaacgtcga gcttccggct ctgctgcgcg gtgagagtag cgcggaaagt cgtaacgggg
    16441 cgaaagcgtt gctcgaacag ttagggctgg gtaaacgtct ggatcatctt ccggcacagc
    16501 tttccggcgg tgaacagcaa cgagtggcgc tggcacgagc ctttaatggt cgacctgatg
    16561 tgctgtttgc cgacgaaccc accggcaacc ttgaccgcca gacgggcgat aaaattgccg
    16621 acctgctgtt ttccctcaac cgtgaacatg gcaccacgtt gattatggtg acccacgacc
    16681 tgcaactggc ggcacgctgc gaccgctgct tacggctggt gaacgggcag ttgcaggagg
    16741 aagcatgatt gcacgttggt tctggcgcga atggcgttcg ccgtcgctat taattgtctg
    16801 gctggcgcta agcctggcgg tggcctgcgt gctggcgctg ggcaatatca gcgatcgcat
    16861 ggagaagggc ttaagccagc aaagccgtga gtttatggcg ggcgatcggg cgttgcgcag
    16921 ttcacgcgaa gtgccgcaag cgtggctgga ggaagcgcaa aagcgcggcc tgaaagtcgg
    16981 caagcagctg actttcgcca caatgacctt tgcaggcgac acaccgcagc tggcgaacgt
    17041 caaagcggtg gatgatatct acccgatgta tggcgatctg caaactaatc cccctggcct
    17101 gaaaccgcag gcgggcagcg tattgctggc cccacgcctg atggcactgc ttaacctgaa
    17161 aacgggcgac accattgacg tgggcgatgc caccttgcgg attgccggag aagtgattca
    17221 ggaaccggat tccggtttta accccttcca gatggctccg cgtctgatga tgaatctggc
    17281 ggatgtcgat aaaaccggag ccgtgcagcc ggggagtcgg gtcacctggc gttataaatt
    17341 cggcggcaac gagaaccagc tcgacggcta tgataaatgg ttgttacctc agcttaaacc
    17401 cgaacaacgc tggtacggtc tggaacagga cgaagacgcg ctggggcgat cgatggaacg
    17461 ctcgcaacag ttcctgctgc tttcggcgct tctgaccttg ctgctggcag tggcagcggt
    17521 ggcggtagcg atgaatcatt actgtcgcag tcgctacgat ctggtggcga tcctcaaaac
    17581 gctgggggca gggcgagcgc aactgcgtaa gctaatcgtc ggtcagtggt tgatggtgct
    17641 gacgctttca gccgttaccg gtggggccat aggcctgttg ttcgaaaacg tgttgatggt
    17701 gctgctcaag cccgttctgc ctgctgcact accgccagcc agcctctggc cgtggctgtg
    17761 ggcgcttggc accatgacgg tcatctcgct gctggtgggg ctacgaccat accgcttgtt
    17821 gctggcaacg cagcctttac gcgtattacg taatgatgtg gtagcgaacg tctggccgct
    17881 gaagttttat ctgccgattg tcagtgtggt ggttgtgctg ctgctcgccg gattaatggg
    17941 tggcagcatg ctgctttggg cggtgctggc gggcgcggta gtactggctt tgctgtgcgg
    18001 tgtgctgggc tggatgcagc tgaatgtact tcgccgcatg acgctgaaat cgctacctct
    18061 gcgcctggcg gttagccgcc tgttacgtca gccgtggtca acgttaagcc agctttcggc
    18121 attttcgctc tcctttatgc tgctggcgct gttgctggta ttgcgtggcg atctgctcga
    18181 ccgctggcag cagcagctac cgccagaaag cccgaactac tttttaatta acatcgccac
    18241 agaacaggta gcaccgctaa aagcgttcct cgcggaacat catatagtcc cggaatcatt
    18301 ttatccggta gtgcgggcgc ggctgacggc gattaacgat aagccgacag aaggtaatga
    18361 agatgaggcg cttaatcgcg aactcaatct tacctggcaa aatacgcggc ccgatcataa
    18421 tccgattgtc gccggtaact ggccgccaaa agccgatgaa gtgtcgatgg aagaggggct
    18481 ggcgaaacgc ttaaacgttg ccctcggtga taccgtgact tttatgggcg atacccagga
    18541 gttccgcgct aaagtgacca gcctgcgcaa agtggactgg gaaagtctgc ggcctaattt
    18601 ctattttatt ttcccggaag gggcattaga cgggcaaccg cagagctggc ttaccagttt
    18661 ccgctgggag aatggcaacg gcatgttgac gcaactcaac cgccagttcc cgaccattag
    18721 cctgttagat attggcgcga ttttaaaaca ggtcggtcag gtgctggagc aggtaagtcg
    18781 ggcgctggag gtgatggtgg tgctggtcac cgcctgcggt atgttgctgt tgctggcaca
    18841 ggtgcaggtg ggaatgcgtc agcgtcatca ggagctggtg gtgtggcgca cactcggtgc
    18901 ggggaaaaaa ctgctgcgta ccacgttgtg gtgtgagttc gccatgcttg ggtttgtttc
    18961 tggcctggta gccgcaattg gtgcggaaac ggcgctggca gtgttgcagg cgaaagtgtt
    19021 tgatttcccg tgggagccag actggcgatt gtggattgtt ctgccgtgca gcggtgcact
    19081 gctgctgtcg cttttcggcg gctggctggg tgcgcgactg gttaagggta aggcgctgtt
    19141 caggcagttt gcggggtgat gacaggtgtt agattgcctg atgcgctacg cttatcaggc
    19201 ctacgaagag cgtgcaatat attgattttg caagattgtg taggccggat aaggcgttta
    19261 cgccgcatct ggcatgaaca gtgtgcattt tggcaataaa tatgaaacca actgtcatgt
    19321 cggtttttta tatctgtatt ctggtgcaac cattctgttc tcacgacaat cgccgtttca
    19381 gaataccgcc catcactgcg tcttaaccca ttccgccact tccgcccact caccgcgaaa
    19441 gacaactttt tccgcttttt tctcaagctg atagcgatac atcgggtcgt aatattcttt
    19501 aagtaacggc accagccagg ccagatgacc gtcggtgctg tcggtggtga gttgcgttgt
    19561 cagcgccgta tccagctggg cagcgagttc gttatagcgc tgtaacccca gccgacgctt
    19621 aatagccgaa agtccgtgat gcaggtattc gcaatattcc tgccagccct gttcgtcgcc
    19681 gtacgcgtgg gtaaaatcat gatgcatacg caagaaatac tcttcgttca ggcgctcaag
    19741 acggatctca aacggatctt ctaccaccgc aatcgccgcc tgagtcattc gctcgcgcag
    19801 gcattccggc aggtgattcg aaccgatcat ccggctttcg tcttccagca cccacaggcg
    19861 caaatcctga cgggcgtcgg tttttagcat ttcggcagcc agcaggtttt caaaactcgc
    19921 ctggctaagt tgtggttgta aggtgcgacc aaacgccgaa ccgcgatgac gcgctaaccc
    19981 ttccagatca acaccgttcg gctgttgctg cactaacagc gttttaccgc tgccggtaca
    20041 accgccaatc agcactatcg gtttttgtgc cagttcaata gtcgcctgaa tcgcggcttg
    20101 gcgcagtgcc ttataaccgc cttccaccag cggataatca atccccgctg catacaacca
    20161 gctttgcaca atatgtgagc gctgaccgcc acgggcgcag cagagaatac cttgcggatt
    20221 ttgcaggcac gctgcccgcc aggcgtccat gcgctgctga cgaatttcac ccgccaccag
    20281 tttatgcccc agcgccagcg ccgcgtctga gccttgctgt ttataacaag tgccaacggc
    20341 ggcgcgttca tcgttattca ttaacggcag gttgatagcg gcgggcattg cgccttgctc
    20401 aaactcgata ggggcgcgaa catcaataat gggcgtatca gcaatcagca gggcacgata
    20461 gtcctgttcc gtgtgtctct cttgcatagt taaaagtgaa cctcaaatca gcttgcgcgc
    20521 tattttacgc gccaacgcgc aaggaaactt gatttttaac tgcgtgggtt gccgaaaatt
    20581 tctaaaaatc cgctgatttc cggcctgcgc tgggtaaaca aggtcacaat atcttctacc
    20641 gctttgccgc tgccaaattt gcgccatgcc agactcaatg gcgaaggagg gcgcatcgtt
    20701 gggattaccc ggctgaccag ttgttgatta tcgatcattg actggcaaag tgattttggc
    20761 aaaaaaccaa tgccaacacc cgccagatgg gcggcgattt tcgtttccat atcgggaaca
    20821 ataatctctt tttgccccgg caatcgccag gcgacgcgtt tggttaaggt tcgggcgctg
    20881 tcttcaatat tgaccgccgg aaagcgccgc aactgcgctt ctgttagcgg ctcttcaacg
    20941 ttcgccagcg gatgatccgc cgccatgaca aagcgccatt gcaccgatcc taagggatca
    21001 agactaaagg tatttgccag cgcctcagtt cccgtgaccc cgatagccag cgaaaaacct
    21061 tcgtacaata gcgagtccca gacgcccata tagatttgtc gggagatgtg aaactgggta
    21121 aaggggtaac gctcattcag ccacgccagc aactgggcga cggcctgggg gttatagagc
    21181 aggttgttga tgacaatatt cacctggcgt tccacgccat cattcacctg ttgcagctcg
    21241 ctgggcatac tttccagcca gctcagccag tctctggcct gggaaagtag atgctcgcca
    21301 gccgctgtca acgtcacgct gcgagtcgtg cggaaaaaca gcgccactcc ggtattctct
    21361 tccagaagtt taatgcgata actgatcgtc gccgtggttt tacataatcg ttctgccgct
    21421 tttgaaaaac ttcctgtttc agcaaccgca atgaaagtcc gcaaggtttc tggatcgaac
    21481 atcttcaggt atcccctttt aaatccgcaa gttgcgtgat tttcttatcc tctgatttat
    21541 cagtattttt acatgataac cctgttcaat ttgtggacta aatctagttt tggaaaaata
    21601 ttccaacttt tgtattgatg ttgttctctt aaggttttag attgcctgtt attgaaacca
    21661 agctgaccgg tcggcggtgg ttgaacggaa ttatgttaca aggacaaaaa gatgaaactt
    21721 caggtattac cgttaagtca ggaagccttt agtgcttatg gcgacgtaat cgaaacgcag
    21781 caacgggatt ttttccatat taacaatggc ctggtggagc gttaccacga tttggcgctg
    21841 gttgagattc ttgagcaaga ccgtacgctt atcagcatta accgcgcgca accggcgaat
    21901 ctgccgctga ccattcacga actcgaacgt catccgctgg gcactcaggc ctttatcccg
    21961 atgaaaggtg aagtgtttgt ggtggtcgtg gcgttaggcg acgacaaacc agacctgtca
    22021 acgctgcggg cgtttatcac caacggtgaa cagggggtga attaccatcg taacgtctgg
    22081 catcacccac ttttcgcctg gcagcgtgtc accgattttc tgaccatcga tcgcggcggc
    22141 agtgacaact gtgatgttga aagtattcct gaacaggaac tctgttttgc gtgacgcctg
    22201 caaccgactt gcataagata aactaattgt tcattgttta tgctcacttg taggtcggag
    22261 ttaacgtagg tatgacggaa gttagacggc gcggcaggcc aggacaggcg gagcctgtgg
    22321 cacagaaggg cgcacaggcg ttagagcggg gaattgcgat tctgcaatat ttggaaaaaa
    22381 gtgggggaag ttcgtcggtt agcgatattt ctctcaatct ggatttgccg ctctccacga
    22441 cctttcgctt gctgaaggtt ttacaggcag cggattttgt ctatcaggac agtcaattag
    22501 gctggtggca tataggatta ggtgtcttta acgtcggtgc ggcgtacatc cataaccgcg
    22561 atgtcctctc cgtcgccggg ccgtttatgc gccgcctgat gttactttcc ggcgaaacgg
    22621 tcaatgtcgc gatccgtaac ggcaatgaag cggtattaat tggtcagtta gagtgtaaat
    22681 cgatggtcag gatgtgtgcg ccactgggca gtcgtctgcc gctgcatgct tccggtgcgg
    22741 gcaaagcgct gctttatccg ctggcggaag aggagttgat gagcatcatt ctgcaaaccg
    22801 gtttgcagca gtttacgcca actacgcttg tggatatgcc caccttgctg aaggacctgg
    22861 aacaagcgcg tgaactgggc tataccgtag ataaagaaga gcatgttgta ggtctgaatt
    22921 gcatagcttc agcaatttac gatgatgtcg gtagcgttgt tgccgctatc tccatctccg
    22981 ggccttcatc aagactgaca gaagatcgtt ttgtcagtca gggtgagctg gtcagagaca
    23041 ccgcccgcga tatcagcacg gcgttgggac tgaaagcaca tccatagtat ctgtcgcatc
    23101 ccgctctgcg gagcgggttt ttttgacaaa atttgaaagt tggaaaattt ttccaattaa
    23161 tagaggtagg aataaaatgg caaaaatgag agccgttgac gcggcaatgt atgtgctgga
    23221 gaaagaaggt atcaccaccg ccttcggcgt tccgggagct gcaatcaatc cgttctactc
    23281 agcgatgcgt aagcacggcg gtattcgtca cattctggcg cgtcatgtgg aaggtgcttc
    23341 gcacatggcg gaaggttata cccgcgcaac ggcaggaaat atcggcgtat gtctggggac
    23401 ttccggtcct gcgggcacgg acatgatcac cgcgctctat tccgcttctg ctgattccat
    23461 tcctattctg tgcattaccg gccaggcacc gcgcgcccgt ctgcataaag aagattttca
    23521 ggccgtagat attgaagcaa ttgctaaacc ggtcagcaaa atggcggtta cagttcgtga
    23581 agcggcgctg gtgcctcgcg tgctgcaaca ggcatttcac ctgatgcgtt ctggtcgtcc
    23641 gggtccggta ctggtggatt taccgttcga cgttcaggtt gcggaaatcg agtttgatcc
    23701 tgacatgtac gaaccgctgc cggtctacaa acctgctgcc agccgtatgc agatcgaaaa
    23761 agctgtagaa atgttaatcc aggccgaacg tccggtgatt gttgccgggg gcggggtaat
    23821 taatgctgac gcagctgcac tgttacaaca gtttgctgaa ctgaccagcg ttccggtgat
    23881 cccaacgctg atgggctggg gctgtatccc ggacgatcat gaactgatgg ccgggatggt
    23941 gggtctgcaa accgcgcatc gttacggtaa cgcaacgttg ctggcgtctg acatggtgtt
    24001 tggtatcggt aaccgttttg ctaaccgtca taccggttcg gtagagaaat acaccgaagg
    24061 gcgcaaaatc gttcatatcg atattgagcc gacgcaaatt ggccgcgtgc tgtgcccgga
    24121 tctggggatt gtctctgatg ctaaagcggc cctgacgctg ctggttgaag ttgcgcagga
    24181 aatgcaaaaa gcagggcgtc tgccatgccg taaagagtgg gttgctgagt gccagcagcg
    24241 caaacgtact ttgttgcgta aaacacactt cgacaacgtt ccggttaaac cgcagcgtgt
    24301 gtatgaagag atgaacaaag cttttggatg tgatgtttgc tatgtcacca ccattggtct
    24361 gtcgcaaatt gccgctgcgc aaatgctgca tgtctttaaa gaccgccact ggatcaactg
    24421 tgggcaggca ggcccgctgg gctggaccat tccggcggcg ctgggggttt gtgccgctga
    24481 tccagagcgc aaagtggtgg cgatttccgg tgacttcgac ttccagttcc tgattgaaga
    24541 attggccgtt ggcgcacagt tcaacattcc gtacatccat gtactggtca ataacgctta
    24601 tctggggctg attcgccagt cgcagcgcgc gtttgatatg gactactgcg tgcaactcgc
    24661 tttcgagaat atcaactcca gcgaagtgaa cggttacggc gtcgaccacg taaaagtagc
    24721 ggaaggttta ggttgtaaag cgattcgcgt cttcaaaccg gaagatattg cgccagcctt
    24781 tgaa
//
LOCUS       DAKISA010000060        24434 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.060, whole genome shotgun sequence.
ACCESSION   DAKISA010000060 DAKISA010000000
VERSION     DAKISA010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24434
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.060"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            224..394
                     /locus_tag="OVW45_002515"
     CDS             224..394
                     /locus_tag="OVW45_002515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5526937.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(452..1225)
                     /gene="zupT"
                     /locus_tag="OVW45_002516"
     CDS             complement(452..1225)
                     /gene="zupT"
                     /locus_tag="OVW45_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCV5526938.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            1368..2156
                     /gene="ygiD"
                     /locus_tag="OVW45_002517"
     CDS             1368..2156
                     /gene="ygiD"
                     /locus_tag="OVW45_002517"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCV5526939.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLASVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            complement(2194..3354)
                     /locus_tag="OVW45_002518"
     CDS             complement(2194..3354)
                     /locus_tag="OVW45_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCV5526940.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKRTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFIE"
     gene            complement(3360..4031)
                     /locus_tag="OVW45_002519"
     CDS             complement(3360..4031)
                     /locus_tag="OVW45_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCV5526941.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            complement(4179..5660)
                     /gene="tolC"
                     /locus_tag="OVW45_002520"
     CDS             complement(4179..5660)
                     /gene="tolC"
                     /locus_tag="OVW45_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCV5526942.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            5865..6494
                     /gene="nudF"
                     /locus_tag="OVW45_002521"
     CDS             5865..6494
                     /gene="nudF"
                     /locus_tag="OVW45_002521"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCV5526943.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            6495..6917
                     /locus_tag="OVW45_002522"
     CDS             6495..6917
                     /locus_tag="OVW45_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCV5526944.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            6942..7769
                     /gene="cpdA"
                     /locus_tag="OVW45_002523"
     CDS             6942..7769
                     /gene="cpdA"
                     /locus_tag="OVW45_002523"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCV5526945.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            7769..8350
                     /gene="yqiA"
                     /locus_tag="OVW45_002524"
     CDS             7769..8350
                     /gene="yqiA"
                     /locus_tag="OVW45_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCV5526946.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            8379..10271
                     /gene="parE"
                     /locus_tag="OVW45_002525"
     CDS             8379..10271
                     /gene="parE"
                     /locus_tag="OVW45_002525"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCV5526947.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(10319..10633)
                     /locus_tag="OVW45_002526"
     CDS             complement(10319..10633)
                     /locus_tag="OVW45_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCV5526948.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(10664..11245)
                     /gene="mdaB"
                     /locus_tag="OVW45_002527"
     CDS             complement(10664..11245)
                     /gene="mdaB"
                     /locus_tag="OVW45_002527"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCV5526949.1"
                     /translation="MSNTLIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            complement(11355..12704)
                     /gene="qseC"
                     /locus_tag="OVW45_002528"
     CDS             complement(11355..12704)
                     /gene="qseC"
                     /locus_tag="OVW45_002528"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCV5526950.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHSIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(12701..13360)
                     /gene="qseB"
                     /locus_tag="OVW45_002529"
     CDS             complement(12701..13360)
                     /gene="qseB"
                     /locus_tag="OVW45_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCV5526951.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            13512..13904
                     /gene="ygiW"
                     /locus_tag="OVW45_002530"
     CDS             13512..13904
                     /gene="ygiW"
                     /locus_tag="OVW45_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCV5526952.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            13957..14439
                     /locus_tag="OVW45_002531"
     CDS             13957..14439
                     /locus_tag="OVW45_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCV5526953.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            14985..17243
                     /gene="parC"
                     /locus_tag="OVW45_002532"
     CDS             14985..17243
                     /gene="parC"
                     /locus_tag="OVW45_002532"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCV5526954.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQSVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASNGDSEE"
     gene            17476..18213
                     /gene="plsC"
                     /locus_tag="OVW45_002533"
                     /gene_synonym="parF"
     CDS             17476..18213
                     /gene="plsC"
                     /locus_tag="OVW45_002533"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCV5526955.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            18288..19700
                     /gene="ftsP"
                     /locus_tag="OVW45_002534"
     CDS             18288..19700
                     /gene="ftsP"
                     /locus_tag="OVW45_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCV5526956.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYDVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            19811..22030
                     /locus_tag="OVW45_002535"
     CDS             19811..22030
                     /locus_tag="OVW45_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCV5526957.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCVRALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRPALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(22073..22330)
                     /gene="yqhH"
                     /locus_tag="OVW45_002536"
     CDS             complement(22073..22330)
                     /gene="yqhH"
                     /locus_tag="OVW45_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCV5526958.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDIKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22381..23307)
                     /locus_tag="OVW45_002537"
     CDS             complement(22381..23307)
                     /locus_tag="OVW45_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCV5526959.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            complement(23507..24334)
                     /gene="dkgA"
                     /locus_tag="OVW45_002538"
     CDS             complement(23507..24334)
                     /gene="dkgA"
                     /locus_tag="OVW45_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCV5526960.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKAPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
BASE COUNT         5692 a         6153 c         6549 g         6040 t
ORIGIN      
        1 aagttgtagc tggcttcggt ttttattttg ccctgctatg caagatgtta attccgcttc
       61 tctttctatg agaaaatttc attaatatca ggcattcttt ttcattatga tcaaaatcat
      121 ataattgttc cgttattatg cgaccatatg aatatatccg aaaaaggata tgtgaaattt
      181 atgcgctaaa tgtttagtat tgttaaatat taagggtaaa aggatgttta tcgcctggta
      241 ctggattgta ttgattgctc tggttgtggt gggttatttc ctgcatttga aacgttattg
      301 tcgggcgttt cgccaggaca gagacgcact gcttgaagcg cggaacaaat acttaaacag
      361 tacgagagaa gagacagccg aaaaggtaga ataaggcttt cgtgaagctg gcagaagttg
      421 tccatgtgct ggacaacttc tgatgttgtt tttaaccaat tcccgccgtt tgtagcagca
      481 ctaaactgaa tcccatcact gacattccac atagtacgcc atagctgggg ttattattag
      541 ggtcaatctc tttggcgagc ggcattaatt catcaaccga gagcgccacc ataattcctg
      601 caaccgccgc catgattgcc gccatgacca ccggggaaat catgctaccg aggattaacc
      661 acgccagcac accaccaaga atttctgcta gtccagaaat ccccgcccac agaatcgcgg
      721 tgcgtttaga ccccgttgcc gcataaaccg ggcctgccac tgccagacct tcagggatat
      781 tgtgcaacgc gacggccagt gcgatgccaa atcccagctc caggttgctg ctcgccgtga
      841 caaaggtggc aatcccttcc gggaagttat gcagactgat gccgagagtg agcagaatgg
      901 ctgtgcgctt gatcgatttt ggcaacggct gcaccgattt ttgcattaaa tcctgcggat
      961 gcgcatgtgg caacatgcgg tccaggccaa aatagccaag cagaccgaag ataaacattc
     1021 cataacccaa cacaggcgac attccttcag ctgctagtgc ggcaggaagc atttccatta
     1081 atgagatgag caacatgatc cccgccgcaa atcctagcga aaacgccagt aagcggttcg
     1141 agggtttttg cccgagaacg ccgagaaacg cgccaataaa cgtggctgcc cccgccagta
     1201 tggtcagaat gagaggtact gacatcgaca actccttata aatcttcacc ttcaggcaga
     1261 tcgtcaaaat aactgatgat catcatcatt gttatccggg ttcttactcc gccgaacaag
     1321 cgtattgtga ggatatcaaa atgacacctt tagttaagga tatcatcatg tcttcaacac
     1381 gtatgccagc attgttttta ggtcacggta gtccgatgaa cgtgctggaa gataatttgt
     1441 atacccgcag ctggcagaag ttggggatga cattgccacg cccgcaagcg attgtggtgg
     1501 tttcggctca ctggtttacc cgtggaacag gagtgaccgc gatggagacg ccgcccacga
     1561 ttcatgactt tggtggcttc ccgcaggcgc tgtacgatac gcattatcct gctccgggtt
     1621 cgcctgtgct ggcacagcat ctggttgagc tgttagcgtc ggtcccggtg acgctggata
     1681 aagaagcctg gggctttgac cacggttcct ggggcgtgct gattaagatg tatcctgacg
     1741 ccgatatccc gatggtgcag ttgagtatcg acagtagcaa acctgccgcc tggcatttcg
     1801 aaatggggcg caaactggca gcgctgcgag atgaaggaat aatgttggtc gccagtggca
     1861 acgtggtgca taacctgcgt acagtgaagt ggcacggtga tagttcacca tatccttggg
     1921 cgatgtcgtt taatgagtat gtgaaagcga atctgacgtg gcaaggggcg gtagagcagc
     1981 atcctctggt gaattacctt gaccatgaag gtggcgcgtt atcgaaccca acgccggagc
     2041 actatctgcc gttgttgtat gtcttaggtg cgtgggatgg gcaggagcca attaccattc
     2101 cggtcgatgg tatagaaatg ggcagcctga gtatgctgtc ggtgcagata ggttaacaat
     2161 taccggatgg cgaatgccat ccggcatcat tgattactca ataaaaatat gtggataaaa
     2221 ccgagacata tcctgggtga tcaacgcacg gtcttcacga atgccaattc cggcaggttg
     2281 atcgttcacc agccagctac caatcagcat atagctgtcg ccgaatttcg gtaacgggtg
     2341 gaattgctga acaatcatcc cttcttcgcc atacggacct tccgctgctt caatggtttt
     2401 gccgttctca atgatcgaca cgtttgcgcc ttcacgggag aagatcggtt taaccacata
     2461 tttttccatt tgcggatgat catcttccgc aaaataagcg ggcagcaggt tcgggtgatt
     2521 cgggaacatc tcccacagta gcggtagaag cgctttgttg gagataatgc tcttccacgc
     2581 cggttccagc cagcgtacgc ctgcatcctc cagcttggtg gagaacatct cacgcaacat
     2641 aaattcccat ggatacagct tgaacaggtt ggaaattacc tgatcctgta aatccgtgaa
     2701 ctgacctttt tcacctaacc cgatatcgtc aatgtagagg aactcggtag cgatttcagc
     2761 ttccgtcgcg cagtcctgca aatactgaat ggttccgcga tcttccaccg tgtcgcgaca
     2821 gcaggtgaga tgcagcaact ggaagccata ctgttcacgc aactcgacaa agcgatcgat
     2881 tagcttttct tgcagactgt taaactggtc gctgccctcc ggcaagttac cggcgttaag
     2941 ctgatcttcc agccagatcc actgaaagaa cgccgcctcg tatagtgacg ttggtgtatc
     3001 ggcgttattt tccagcagtt taggttcgcc agtgccatcc catgccagat caagacgcga
     3061 ataaagcgat ggctggtgag tcagccatga ctgacgcaca aaactccagg tgcgttttgg
     3121 aatgcggaat ttggtcatca gctcatcgct ggcgatcact ttttccacca ctttcaggca
     3181 catctggtgc agttcggcgg tgacttcttc cagcttttca acctgggcga gggtcaactt
     3241 gtagtaagca tcttcacacc agtacggctc gccgtacatg gtgtgaaaat tgaaaccgta
     3301 ttcgtgggct ttttcgcgcc agtccgggcg ctcggtaata ctgactcttt ccatcggtat
     3361 cagccaccca ttgaacgaga agaggtgccg gttgcgctac gctgcatagt gctttgtttg
     3421 gcaacagatt caccaaaacc gccacgggta acggtagtag tggtcgccgg ttttggtgcc
     3481 atggctgtct tcggtacggt catggtgcgg cctggctggg ctgcgccata gtttttaccc
     3541 gtcgcgtcgg tatatttacc gtaagccggg ctggctgggt ttttcgagga gaacagcggc
     3601 tgctgtgcaa atcccgcgcc gccgcccatc agacgcccca tcatgtaacc ggccatcagc
     3661 ggcatccaga aactcccgct ggattgctgg gcctgcgcct ggttttctgg tgccatgcca
     3721 gcctgggctg gtgcctgctg gcactgacct tcaccaaatt cagcaacaca gtcttcacgg
     3781 gtggcgtatt tcggcgcagt acgttcggct tctttcagcg cattgttgta cgcggtggta
     3841 cattcggcgc ttttgcctgg gtttgcagct gaacagtcgt cagcattttg atagagagac
     3901 actgtttcat cactcttttc acagccagcc agcataaaaa cagtggcaac cgcgagagcg
     3961 actggtgtca gatggcgtgc gctccagttt ttgcggaacg atgcgtggcg tatggatttt
     4021 gtccgtttca tttttgtctt ccgggaccag tggtaaatac ccatcagaat agaggatggc
     4081 tggtcgaaat tgaagcgaga agggggaaga atgcggcaga taacccgtat ctttacgttg
     4141 ccttacgttc agacggggcc gaagccccgt cgtcgtcgtc agttacggaa agggttatga
     4201 ccgttactgg tggtagtgcg tgcggatgtt tgctgaacga ctggtgccgg gctatcaggc
     4261 gcataaccat cagcaatagc attctgttcc ggcgtttgcg gtgcaacgtt ttccggatta
     4321 gtggaaaccg gtttgctcag cgcattgttc agtgccagca gatcctgctc gttcaacgta
     4381 cccagagctg acttaatatt caactggtta atcaggtagt tataacgcgc atttgccagc
     4441 tcttgcttag cgttgtacag cgtggtggtt gcatccaaca catcaacaat ggtacgcgta
     4501 ccgaccgagt agcccgcttc catcgcgtct aatgagcttt gagcggaaac tacggcttgt
     4561 ttgtaggcgt taatgctact gatagatgca ttaatgttgt tgaaggagga acgtacggtt
     4621 tgcacgacgc tacgatgcgc gctttccagt tgctcgctgg caccaacaaa gttgtactgg
     4681 gcctgtttca cctgcgagtt aaccattccg ccctgataaa tcggcagcga gaagctcagg
     4741 cccactttgt tctggcccat attgctgtcg tcatactggg taccagcggc accacgggtt
     4801 ttcgaaccgc tataagaggt gtcagaaatc ccggtagaag ccgttaaatc cagcgtcggt
     4861 aagtgaccat cctgcgcctg gcgaatttgc tcgcgcgcca ggtcctggct caagcgtgcc
     4921 tgtaacagcg acaggttgcg tttttcggct tctttcagca gcgcgttaac cggctgtggt
     4981 ttgtcggttt taaagttttc gacattcagc gccgccagtt ccggatagta gttaccggtg
     5041 atctggcgca gctgctctac cgcgttatca aggttattac gtgcggtcac ttcgttcgcc
     5101 agcacggtat cgtactgcgc gcgggcgttc tgcacgtcgg tgatcgctac caagcccacg
     5161 ttaaaacgtt gggtggtttg atctaattga cggtagatcg cttctttttg tgcctgtgta
     5221 taggaaagaa cgtcaatagc attcaacacg ttgaaataag cggtcgcggt gttgaggatc
     5281 aaggtttgct gatcggtctg atatgtgacg tcctgaatcc ctgctgcttt ttcctgcagc
     5341 gttaacgcac gccatttcga catatcaaaa atggattgag ttaactgcag ggacgcactg
     5401 gtcgcgttag agttgatgcc gttcgcgtcg cggtagccgt tgctataggt gtaatctgca
     5461 cctaaaccta gctgtggcag taatggactg cgcgcttcat taattttttc aaaggcagca
     5521 tcacgatcgg cggcagactt acgcaattcc gggttactaa ggcgtgcttg ctgataaact
     5581 tgcatcaggt tctcggcctg gctcaacgaa ctgaacccag aaaggctcag gccgataaga
     5641 atggggagca atttcttcat ttgcattcct tgtggtgaag cagtatttag cgcgatcaaa
     5701 ctgtaaaatt attgcggatt ctagcagaag ccgctaccgc aaaaggttgg cgttacgtgc
     5761 catagggcgt taatgtgcct caatttaaca catgactgct caaacggcag tcaaacgtcg
     5821 ctgaaattca catttaattc actattagtg ccaggacatt aacaatgctt aagccagaca
     5881 acctgcccgt tacatttggc aaaaacgatg tagaaattat tgcacgagaa acactttatc
     5941 gcggcttttt ttcattagat ctttatagat ttcgtcatcg tctattcaac gggcaaatga
     6001 gtcatgaggt acggcgggaa atttttgagc gcggtcacgc cgcagtcttg ctaccctttg
     6061 acccagtgcg cgatgaagtt gtgctgattg agcagattcg gattgccgca tacgacacca
     6121 gtgaaacccc ctggctactg gagatggttg ccgggatgat agaagagggt gaaagtgtgg
     6181 aagatgttgc ccgtcgcgaa gcgattgaag aggcgggact gatagtcaaa cggaccaaac
     6241 cggtgttaag tttcctggca agcccggggg gcaccagtga gcgttcgtca attatggtgg
     6301 gcgaagtgga cgccacgacc gcaagcggta ttcatggtct ggctgatgaa aacgaagata
     6361 ttcgcgttca tgtggtaagc cgggaacagg cataccagtg ggtagaagag gggaaaatcg
     6421 acaacgcagc gtcggtcatc gctttgcaat ggctgcagct gcatcatcaa gcgttaaaaa
     6481 atgagtgggc ataaatgaag cgttacacac ctgactttcc tgaaatgatg cgcctgtgcg
     6541 agatgaactt ttcacaattg cgccgtttgt taccgcgcaa tgacgcaccc ggcgaaactg
     6601 taagctatca ggtggcaaac gcacaatatc ggctgacgat tgtggaatcg acccgataca
     6661 ctaccctggt gacaatagaa cagactgcgc ccgcgatcag ttactggagc cttccgtcaa
     6721 tgacggtgcg tctgtatcat gacgcgatgg tggctgaagt gtgttcaagc cagcagattt
     6781 ttcgcttcaa agcgcggtat gattatccta ataaaaagtt gcatcaacgc gacgaaaagc
     6841 atcaaattaa tcagttttta gcggactggt tgcgatactg tttagcacat ggagcgatgg
     6901 cgattccggt ttactagcat cgtgaaacct aaggacacca tttggaaagc ctgttaaccc
     6961 ttcctctggc tggtgaggcc agagtcagga ttttacaaat taccgacact cacctgtttg
     7021 cacaaaagca cgaagctctg ttaggggtaa acacctggga aagctaccag gcggtgctgg
     7081 aggcgattcg tccacatcag cacgaattcg acctgattgt cgcgacaggt gatttagcgc
     7141 aggatcaatc ctctgcggcc tatcagcatt tcgctgaagg catcgcaagt tttcgtgcgc
     7201 cctgcgtctg gctgccgggc aaccacgatt tccagcccgc gatgtacagc gcgttacagg
     7261 atgcgggtat ctccccggcg aagcgggtgt ttattggtga gcaatggcaa atcctgttgc
     7321 tggatagcca ggtgtttggc gtgccgcacg gtgagctgag cgagtttcag cttgagtggc
     7381 tggaacgtaa actggccgat gcgccagaac gccatacgtt gctgctgctg catcatcatc
     7441 cgctacctgc gggttgtagt tggctcgatc aacacagtct gcgtaacgcg ggcgaactgg
     7501 ataccgtgct ggcgaagttt ccgcacgtca aatacttgct gtgcggtcat attcatcagg
     7561 agctggatct cgactggaac ggtcgccgcc tgctggcaac gccgtcgacc tgtgtgcagt
     7621 ttaagccgca ctgttccaac tttacgctgg atactatcgc gcccggctgg cgtactctcg
     7681 agttacatgc tgatggcacg ctgaccaccg aggtgcatcg cctggcggac acacgtttcc
     7741 aacctgatac cgcttcagaa ggctactgat gtctacgctt ctttatttac acggtttcaa
     7801 cagctcgccg cgctctgcaa aagcgagctt gttaaaaaac tggctggcgg aacatcaccc
     7861 tgacgttgag atgatcattc cgcagttgcc gccgtatcct tccgacgcgg cagaactgct
     7921 ggaatccatt gtcctggaac atggcggcga ttcgctgggt attgtcggtt cgtcactggg
     7981 gggatattac gccacctggt tgtcacaatg ttttatgctg cccgcagtgg tggtaaaccc
     8041 ggcggtgcgc ccgtttgaac tgctgacgga ctatctcggt cagaacgaga acccatacac
     8101 cgggcagcaa tatgtgctag agtcacgcca tatttacgat ctcaaagtca tgcagattga
     8161 cccgctggaa gcgccggatt tgatctggct gctgcaacag acgggagatg aagtgctgga
     8221 ttaccgccag gcggtggcgt actacgcttc ctgccgccag actgtcatag aaggcggcaa
     8281 ccacgcattc acgggcttcg aagattattt caacccgatc gtcgattttc ttggtctgca
     8341 ccatctctga cgatcaactc gaattgctaa cttaaaccat gacgcaaact tataacgctg
     8401 atgccattga ggtactcacc gggcttgagc cggttcgccg ccgtccgggg atgtataccg
     8461 ataccactcg ccctaaccat ttggggcaag aagttattga taacagtgtc gatgaagcac
     8521 tggcgggtca cgcaaaacgc gtggatgtaa tcttacatgc tgaccagtcg ttagaagtta
     8581 ttgacgatgg gcgcgggatg ccggtggata ttcacccgga agagggtgtc ccggcggttg
     8641 aattgattct ttgccgtctg catgcaggcg gtaaattctc taacaaaaat taccagttct
     8701 ctggcggcct gcatggcgtg gggatttcgg tggttaacgc cctgtcgaag cgcgttgaag
     8761 ttaacgtgcg ccgcgatggt caggtttata acatcgcctt tgaaaatggc gaaaaggtgc
     8821 aggatttaca ggttgtcggc acttgcggta aacgcaatac cggtaccagc gtgcacttct
     8881 ggccggatga aaccttcttt gacagcccgc gattttctgt ttcacgcctg acgcatgtgc
     8941 tgaaagccaa agcggtactg tgccccggcg ttgagattac ctttaaagat gagatcaaca
     9001 acaccgaaca gcgctggtgc tatcaggacg gtctgaatga ttacctggcg gaagcggtaa
     9061 acggtttacc gacgctgcca gaaaaaccgt ttatcggtaa tttcgctggc gatactgaag
     9121 ctgtggactg ggcgctactg tggctgccgg aaggcggtga actgctgacc gaaagctacg
     9181 tcaacctgat cccaacgatg cagggcggta cccatgttaa tggcctgcgt cagggcctgt
     9241 tggacgcgat gcgtgagttc tgtgaatatc gcaacattct gccgcgcggc gtgaagctgt
     9301 cggcggaaga tatctgggat cgctgcgcct atgttctgtc ggtgaaaatg caggatccgc
     9361 agtttgccgg gcagacgaaa gagcgtctct cttcgcgtca atgcgcggca ttcgtttctg
     9421 gcgtggtgaa agatgccttt atcctgtggc tgaaccagaa cgttcaggcg gcggagttgc
     9481 tggcagagat ggcgatttcc agcgcccagc gtcgtatgcg tgcggctaaa aaagtggtgc
     9541 gtaaaaagct gaccagcggc ccggcgctgc ctggcaaact ggctgattgt accgcgcagg
     9601 accttaaccg taccgaactg ttcctcgtgg aaggtgactc cgcaggcgga tctgccaagc
     9661 aggcgcgcga tcgtgaatat caggcgatca tgccgctgaa aggtaagatc cttaacacct
     9721 gggaagtttc ttccgacgaa gtgctggcct cgcaggaagt gcatgatatt tcggtagcga
     9781 tcggtatcga tcctgacagc gacgatctga gccagcttcg ttatggcaaa atctgtatcc
     9841 tcgcggatgc ggactctgat ggtctgcaca ttgccacgct gctctgcgct ttgttcgtaa
     9901 aacacttccg cgcgttggtg aaacacggtc acgtttacgt cgcactgcca ccgctctacc
     9961 gtattgatct cgggaaagag gtttattacg cgctgacgga agaagagaaa gagggcgtac
    10021 ttgagcaatt aaaacgcaag aaaggcaagc cgaacgtcca gcgttttaaa ggtctggggg
    10081 aaatgaaccc gatgcaattg cgtgaaacca cgcttgatcc gaacactcgc cgtctggtgc
    10141 agttgactat cgatgatgaa gacgatcagc gtactgacgc gatgatggat atgctgctgg
    10201 cgaagaaacg ctcggaagat cgccgcaact ggttgcaaga gaaaggcgac atggcggaga
    10261 ttgaggttta aaggaaagaa cattgcccgg tcaggatggc cgggcaaaca aggcaggatt
    10321 aaatccctgg ctgcagaata cgaatattca tctccagcac gtcgcctttt acggcttcgc
    10381 tgtacgcctt catgtgcggg gtttgcagat gcgcttcaag gtgcgcgatg ctttcccact
    10441 gctcaatcat cacgatagaa tccggtgcca tagactggaa actcacgcca gcagcgcaat
    10501 ccaccattgg cgcatagccg tggcaacctt cttctttcag tacggttgga acgattttag
    10561 caaactgatc caataccgcc tgacggtgat gttgaccagg acgagtacgg atttctgcga
    10621 ttacggtaag catggttaac tccttctaaa gcctgagctc tagttaacca aaaatttcca
    10681 caagatgctt gcgatattct tcagtatagc ggggaacatc aggcattttt atcacgtcat
    10741 tagcgataaa tgtcggcagc ggttccatgc caaggaattg gtttgcttta tggaacggca
    10801 gatacacacc gtcgacgcca acgccgtgga agaattgatc tttttcggtg aaggcttcca
    10861 ttggtgcgtt ccaggtcaga gaaagcatat attttttgcc ctgtaccagg ccgccggaac
    10921 cgtatttttt tgacggatct ttgcgggtac ggccatcgct ggcatacagc gtcccgtgac
    10981 cttcggtgaa tacatcatca atgtattttt tcactgtcca cggcgcgccc atccaccagc
    11041 ctggcatctg ccagatcacc acatcagccc agagaaagtt ttgtacttcc gctttgacat
    11101 cgtagtcgct gtcggcgcga acgatgcgaa catcatgccc gaggtcgcgc agtgtgccat
    11161 ccgcgacttc ggtcagggtg tcgttcagtt gaccattgga gtgggcgaat tttttcgcgc
    11221 cgttgataat cagggtgttg ctcatttttt aacctcaagg tgagacgttt gtcgactata
    11281 gtacgtcaca gagcggtacg gtaaaattag caaaatgtgc aaagtctttt gcgaatttag
    11341 caaaagtctt acccttacca gctcaccttc gcctcaaatc caccttgttc cgcattccca
    11401 aattcaacat tcatgccatg caatttggcg attcgctgga caatcgatag cccaagtcca
    11461 ctgccggtag cggtttgtcc gggggggcga tagaagcgtt cgccaattcg cgccagtgcc
    11521 tctggtgtca caccggggcc gttatccctc acgatgaaat tatcggcatt cagcgtgacg
    11581 tccaccacgc tgccctgtgg actgtagcgc acggcgttat ccagaaggtt tcgcaccaac
    11641 aaacttagca atagcggttg tccagtgcgt ttgatgctgt gggcattgag tgtcagtcgc
    11701 acgtcaattt tcgcctgctg cgccgtgtgg taaatatcca tcaccgacga ttgcaggaga
    11761 tcttcaagcg ggatctccgc gacgtcctga aggttatcca gtgagtccag ccgcgatagc
    11821 gtgagcagtt gatcaaccag acgagtagcg cgatcgatcc cggaatgtaa ttggagcagt
    11881 gcttttttcc gcgcctgcgg atcatcgtca gagagctgcg caacttcggt ttgcactttc
    11941 agcgctgtta acgggctacg tagttcgtga gctgcatcgg aggtaaagcg tcgttctcga
    12001 accatcatcg catgtgtgcg ggcgaacagt tgatttagcg actcaaccag tggtcgcact
    12061 tcgctgggta cgccagtcgc gtttagtggt ttttccgagt cagggtcacg catacgtagt
    12121 gccagcgcca gtttgttcag cggcgcgagt tcacgaccca gtagtaccat catgatgatt
    12181 aacataatcg gcagtgcgac cagccacggg atcaattgcc cggcaacaat cgccagcgcc
    12241 atgtcttcac ggtattccca ttcctggcca acaacgatgc gatatttgcc atcaggtgag
    12301 gtcatccaga caaaacgcca aggatcgtct tcaccgacca gttgcccgtc agcaaaacct
    12361 tcccgttgat agctataggg aatatcttct ccgttatcgc catcattaag gaccattctg
    12421 ccgtcgtggg taaagatggc aaaggtcagc gcatcgtcat caacatgacc gtgttttaat
    12481 ttatttggcg tctgtgccat gcgatccgcc gcgttgattt cgttgagatc gagcgtactt
    12541 aaccgcttgg caaacagcat cagttgggtg tcgaacaatt catcgacgtt atccgttgtt
    12601 tgtttccagg cgacaaagct ggaaagcagc caggtcaccg aggccagaat taaaaagatt
    12661 agcgtcagcc tgacgcgcag actaagacgt tgggtaaatt tcatttctca cctaatgtgt
    12721 agccaatacc atgcacggta cgaataaaat cactaccgag tttgcgtcgc agatgatgca
    12781 catgcacttc aacggcatta ctggtgacct cttcgtccca ggtatacagt ttctcttcaa
    12841 tcagtttgcg cggcagtacc cgaccagcgt tacgcatcag taattccagc agggcaaatt
    12901 cttttggttt cagtgttaag ggttcgccag ccagcgtggc gatacgtttg ccggggtcga
    12961 gcatgacgtt accgtggcgc agctcgttgc tggcctggcc gttggttcgg cgcatcagag
    13021 cctccagcct ggcggcgact tctatcaacg caaaaggttt acacagataa tcgtcagctc
    13081 ccagacgcag cccttctaca cgttccgcta acgcatcacg cgcggtcagg atcagtaccg
    13141 gctcacgctg acctttctct cgccattcgc gcaaaatatc gcgaccatcc atgcctggta
    13201 aggttaaatc caggatcacc gcatcataag gtgcgctata aagcgcctct tttccctgac
    13261 gaccttgtgt aaaccagtcg acactaaaac ccattttact aaggccagtt ttgatgccgt
    13321 cgccaatcag catgtcatct tctatcagta aaattcgcat tttttcatcc ctgcgataac
    13381 cgtatttgct gccagtaaac cgtgttgtaa cgcgctctgt acagcattaa attttatttt
    13441 tttcccttaa gaagttgtta aggactatct tgttaaatgc tcggaaacag acattaaagg
    13501 gagtaataaa catgaaaaaa ttcgcagcag taatcgcagt aatggccctg tgcagtgcac
    13561 cggtgatggc agcagagcag ggcggttttt ctggcccatc ggcaacgcaa agtcaggccg
    13621 gaggattcca ggggccgaac ggcagcgtaa cgactgtaga aagcgcaaaa tccctgcgtg
    13681 acgacacctg ggtaaccctg cgcggcaata tcgttgaacg catctctgac gatctctacg
    13741 tgtttaaaga tgccagcggt actatcaatg ttgatatcga ccacaaacgc tggaacggtg
    13801 tgacggtgac gccgaaagat acggttgaga ttcagggtga agtcgataaa gactggaatt
    13861 ctgttgaaat tgacgtcaaa cagatccgca aagtaaatcc gtaattgtta ccgctcccgg
    13921 gacgcgttcc cgggaataat ttcgcaggga ggcaaaatga caaacctgac actggatgta
    13981 aacattatcg atttcccatc aatccctgtg gcgatgttgc cgcaccgctg tagccctgag
    14041 ttgctcaact acagtgtggc gaaatttatc atgtggcgta aagagacggg gctttctcct
    14101 gttaaccaaa gccagacttt tggcgtcgcc tgggacgacc ctgccaccac cgcaccggaa
    14161 gcgtttcgct ttgatatctg cggcagcgtt agcgaaccga ttcccgataa tcgttatggt
    14221 gtgagcaatg gtgaacttac cggtggacgt tatgccgtgg cccgccacgt tggcgagctg
    14281 gacgatattt cacacacgat atggggcatt attcgccact ggctgcctgc aagcggcgag
    14341 aaaatgcgta aagcaccgat tctgtttcac tacaccaatc ttgccgaagg ggtgacggag
    14401 cagcgactgg aaacggatgt ttatgtgccg ttagcgtgat ggggaatctt taccggagtg
    14461 tgagccaaat cactcgcgat aagtttgagc gaacaatgcc acaggcgtat tgttgttggg
    14521 cttataattt ctccggtaag gaataccatg tatacgcgaa atttattatc cggtgcgccg
    14581 taagccgtct cgggggtacc catgagcaag ccgcacttat ggcaggaggc gcggggcact
    14641 ggtggagaca cagctaaact gggctgaacc atatacttac agtcgactac tttgatgctt
    14701 taggcttccc tggactcgca taacctcaac ttctcgaatc gcccggagcg gacccccatg
    14761 ccgagtggtg tggcatgggc gcaaccttgt gctgccctct atgccgatca agtctacagc
    14821 aaagagttgt atatcaaggc acattgatgc ctttatcgcc gtcggagcac gtccggcggc
    14881 gctattcacg ctgatctcct gtgactcgac gcggcagata atgtagtatc tccggcaata
    14941 ttgccccttt gaaggctggc gaatcagttg aggaatcaga attaatgagc gatatggcag
    15001 agcgccttgc gctacatgaa tttacggaaa acgcctactt aaactactcc atgtacgtga
    15061 tcatggaccg tgcgttgccg tttattggtg atggtctgaa acctgttcag cgccgcattg
    15121 tgtatgcgat gtctgaactg ggcctgaatg ccagcgccaa atttaaaaaa tcggcccgta
    15181 ccgtcggtga cgtactgggt aaataccatc cgcacggcga tagcgcctgt tatgaagcga
    15241 tggtcctgat ggcgcagccg ttctcttacc gttatccgct ggttgatggt caggggaact
    15301 ggggcgcgcc ggacgatccg aaatcgttcg cggcaatgcg ttacaccgaa tcccggttgt
    15361 cgaaatattc cgagctgcta ttgagcgagc tggggcaggg gacggctgac tgggtgccaa
    15421 acttcgacgg cactttgcag gagccgaaaa tgctgcctgc tcgtctgcca aacattttgc
    15481 ttaacggcac caccggtatt gccgtcggca tggcgaccga tattccaccg cataacctgc
    15541 gtgaagtagc tcaggcggca atcgcattaa tcgaccagcc gaaaaccacg ctcgatcagc
    15601 tgctggatat cgtgcagggg ccggattatc cgactgaagc ggaaattatc acttcgcgcg
    15661 ccgagatccg taaaatctac gagaacggac gtggttcagt gcgtatgcgc gcggtgtgga
    15721 agaaagaaga tggcgcggtg gttatcagcg cattaccgca tcaggtttca ggtgcgcgtg
    15781 tactggagca aattgctgcg caaatgcgca acaaaaagct gccgatggtt gacgatctgc
    15841 gcgatgaatc tgaccacgag aacccgaccc gtctggtgat tgtgccgcgt tccaaccgcg
    15901 tggatatgga tcaggtgatg aaccacctct tcgctaccac cgatctggaa aagagctatc
    15961 gcattaacct caatatgatc ggtctggatg gtcgtccggc ggtgaaaaac ctgctggaaa
    16021 tcctctccga atggctggtg ttccgtcgcg ataccgtgcg ccgccgactg aactatcgtc
    16081 tggagaaagt cctcaagcgc ctgcatatcc tggaaggttt gctggtggcg tttctcaaca
    16141 tcgacgaagt gattgagatc attcgtaacg aagatgaacc gaaaccggcg ctgatgtcgc
    16201 ggtttggcct tacggaaacc caggcggaag cgatcctcga actgaaactg cgtcatcttg
    16261 ccaaactgga agagatgaag attcgcggtg agcagagtga gctggaaaaa gagcgcgacc
    16321 agttgcaggg cattttggct tccgagcgta aaatgaataa cctgctgaag aaagaactgc
    16381 aggcagacgc gcaagcctac ggtgacgatc gtcgttcgcc gttgcaggaa cgcgaagaag
    16441 cgaaagcgat gagcgagcac gacatgctgc cgtctgaacc tgtcaccatt gtgctgtcgc
    16501 agatgggctg ggtacgcagc gctaaaggcc atgatatcga cgcgccgggc ctgaattata
    16561 aagcgggtga tagcttcaaa gcggcggtga aaggtaagag caaccaatcg gtagtgtttg
    16621 ttgattccac cggtcgtagc tatgccatcg acccgattac gctgccgtcg gcgcgtggtc
    16681 agggcgaacc actcaccggc aaattaacgt tgccgcctgg ggcgaccgtt gaccatatgc
    16741 tgatggaaag cgacgatcag aaactgctga tggcttccga tgcgggttac ggtttcgtct
    16801 gcacctttaa cgatctggtg gcgcgtaacc gtgcaggtaa ggctttgatc accttaccgg
    16861 aaaatgccca tgttatgccg ccggtggtga ttgaagatgc ttccgatatg ctgctggcaa
    16921 tcactcaggc aggccgtatg ttgatgttcc cggtaagcga tctgccgcag ctgtcgaagg
    16981 gcaaaggcaa caagattatc aacattccat cggcagaagc cgcgcgtgga gaagatggtc
    17041 tggcgcaatt gtacgttctg ccgccgcaaa gcacgctgac cattcatgtt gggaaacgca
    17101 aaattaaact gcgcccggaa gagttacaga aagtcactgg tgaacgtgga cgccgcggta
    17161 cgttgatgcg cggtttgcag cgtatcgatc gtgttgagat cgactctcct cgccgtgcca
    17221 gcaacggtga tagcgaagag taatatgccg gggagggcct gtttcctccc gcttgcaagg
    17281 aacgccggat gaaacgttaa gtcgtcatcc ggcagcgtat tcaacccccg gaaacgataa
    17341 aaattccctt gaaaccgttg tttattcatg cgttgcgatt aacaatacgc ttttccagag
    17401 agcggctttt aacaatgccc taacctgatt tcaggtgacg tacaatgcca gtctctcaga
    17461 ccttcagagg gtgttatgct atatatcttt cgtcttatta ttaccgtgat ttacagcatc
    17521 ttagtctgtg tattcggctc catttactgc cttttcagcc cgcgtaaccc gaaacatgtg
    17581 gccacctttg ggcatatgtt tggccgtctt gcgccgctgt tcggcctgaa agttgagtgc
    17641 cgcaaacctg cagatgctga aagctacggc aatgctatct atatcgctaa ccaccagaac
    17701 aactatgaca tggtgacagc atcgaacatt gtgcaaccgc cgacggtgac ggtaggtaaa
    17761 aagagcttgc tgtggatccc cttcttcggg cagttgtact ggttaaccgg caacttattg
    17821 atcgacagaa acaatcgcac taaagctcac ggcaccattg cggaagtcgt gaatcatttc
    17881 aaaaaacgcc gtatttccat ctggatgttc ccggaaggaa cccgcagccg tggtcgcggc
    17941 ctgctaccgt tcaagactgg agcatttcac gcggcaattg cggcgggcgt cccgattatt
    18001 cccgtgtgcg tctctacaac ttcgaataag attaatctta atcgactgca caacggtctg
    18061 gtgattgtcg aaatgctgcc gccaattgac gtcagtcagt atggcaaaga tcaggttcgt
    18121 gagctggctg cccattgtcg ttcgataatg gaacaaaaaa tcgccgagct cgataaagaa
    18181 gtcgcagaac gcgaagccgc cggaaaagtt taagtcggca atctgtattt ttgcggggaa
    18241 cactttcctg cacggtatta ctttagccag ttttacatgg agcaaatatg tcactcagtc
    18301 ggcgtcagtt cattcaggca tcggggattg cactttgtgc aggcgctgtt cccctgaagg
    18361 ccagcgcagc cgggcaacag caaccgctac ccgttccgcc gctacttgaa tctcgccgtg
    18421 ggcaaccgct gtttatgact gtacaacgtg cgcactggtc atttacgcca gggacacgcg
    18481 cgtcggtctg gggaatcaat ggtcgttacc tggggccgac tatccgcgtc tggaagggcg
    18541 acgatgttaa gcttatttac agcaaccgcc tgacagaaaa tgtctcaatg acggtggccg
    18601 ggctacaggt acctggcccg ctgatgggcg gtccggcacg gatgatgtcg ccaaacgctg
    18661 actgggcacc cgtactgcct attcgccaga acgcagctac tctgtggtat cacgccaata
    18721 ctcccaaccg cacggctcag caggtctata acggccttgc cggaatgtgg ctggtggaag
    18781 atgaagtcag caagtcgctg cctatcccca accattatga tgtggatgat tttccggtca
    18841 ttatccagga taaacggctg gataactttg gtacgccaga atacaacgaa ccgggaagcg
    18901 gcggctttgt tggtgatacg ctgctggtta acggtgtaca aagcccgtac gttgaagtct
    18961 cgcgtggctg ggtgcgcttg cgactgctga acgcgtcgaa ctctcgtcgc tatcaactac
    19021 agatgagcga tggtcgcccg ttacatgtga tttctggcga tcagggattc ctgcctgctc
    19081 ctgtatcggt gaagcaactt tcgctggcac cgggcgagcg ccgcgagatt ctggtggata
    19141 tgagcaacgg cgatgaagtg tcgatcacct gtggcgaagc ggcgagcatt gttgatcgta
    19201 ttcgcggctt ctttgagcca tccagtattc tggtttctac cctggtgcta acgctgcgcc
    19261 caaccggcct tctgccgctg gtcacagaca gtcttccgat gcgcttgctg ccaactgaaa
    19321 tcatggccgg ttcgccgatt cgcagtcgcg atatcagtct gggtgatgac ccgggtatta
    19381 atggacagct gtgggacgtc aaccgtattg atgtcaccgc gcagcaagga acgtgggaac
    19441 gctggacggt acgcgcggac gagccgcaag cgttccatat tgaaggcgtg atgttccaga
    19501 tccgtaacgt gaatggtgcg atgccgttcc cggaagacag aggctggaaa gataccgttt
    19561 gggttgacgg acaagtggag ctgcttgttt atttcggtca gccttcctgg gcgcacttcc
    19621 cgttctactt caacagtcag acgttggaaa tggcggaccg tggctcgatt gggcaactgt
    19681 tagtcaatcc ggtaccgtaa tcttcatact cgcccgagga gggcgagtat ggaatacttt
    19741 tcgatttctc tttcgttccg gcttataatc cccggccttt gattattttt ttatcatttc
    19801 tggaagcaaa atgagctcta tctccctgat ccaaccggat cgcgacctgt tctcctggcc
    19861 gcagtactgg gccgcctgtt ttggaccggc accgtttttg ccgatgtctc gtgaagagat
    19921 ggatcaactt ggctgggata gctgcgacat cattttggtt actggcgacg cgtatgtcga
    19981 tcacccaagc ttcgggatgg cgatttgcgg tcgtatgctg gaagcacagg gctttcgcgt
    20041 cgggatcatc gcccagccgg actggagcag caaagacgac tttatgcgtc tgggtaaacc
    20101 gaatctgttt ttcggtgtta ctgctggcaa catggattcg atgatcaacc gttataccgc
    20161 cgatcgccgt ttacgtcatg acgatgccta cacgccggat aacgtcgcgg gtaagcgccc
    20221 ggatcgcgcc acactggttt atacccagcg ttgtaaagag gcgtggaaag atgtaccggt
    20281 gatcctcggc ggtattgagg ctagtctgcg ccgtaccgca cattatgatt actggtccga
    20341 taccgtgcgc cgttccgtgc tggtggattc gaaagccgac atgctgatgt ttggtaacgg
    20401 tgagcgtccg ctggtggagg tggcgcaccg tctggcgatg ggcgagccga ttagtgaaat
    20461 ccgcgatgtg cgtaataccg cgattatcgt gaaagaggcg ctgcctggct ggagcggcgt
    20521 ggattccacc cgtcttgata cccctggaaa aatcgaccca atcccgcatc cgtatggtga
    20581 agatttgccg tgcgcggata acaaaccggt ggcaccgaaa aagcaggaag ccaaagccgt
    20641 aaccgtgcag ccaccgcgcc cgaaaccgtg ggaaaaaacc tacgtgttgc tgccttcttt
    20701 cgagaaagtg aagggcgata aagtgctgta cgcccatgct tcgcgtattc tgcaccacga
    20761 aaccaaccca ggctgcgtcc gcgcattgat gcaaaaacac ggtgaccgct atgtgtggat
    20821 caacccgcct gctatcccgc tttctaccga agagatggac agcgtttttg cgctgccgta
    20881 caagcgcgtg ccacatccgg cctatggcaa tgcccgtatt ccggcttacg aaatgatccg
    20941 tttttcggtc aacattatgc gcggctgctt tggcggctgt tctttctgtt ctattaccga
    21001 gcacgaaggg cgcattattc agagccgttc ggaagattcg atcattaatg agatcgaagc
    21061 gatccgcgac accgttccag gttttacggg cgtgatttcc gatctcggtg ggccaactgc
    21121 caacatgtat atgttgcgct gcaaatcgcc acgcgctgaa caaacttgtc gccgtttgtc
    21181 gtgcgtttat ccggatattt gtccgcacat ggacactaac cacgaaccga cgatcaacct
    21241 ctatcgccgt gcgcgtgatc tgaaaggcat taaaaaaatc ctcatcgcct caggcgtacg
    21301 ttatgacatc gccgtggaag atccgcgcta tatcaaagag ttggcgaccc atcacgtcgg
    21361 cggttatctg aagattgctc cggaacatac cgaagaaggg ccgttgtcga agatgatgaa
    21421 gccgggcatg ggcagctatg accgctttaa agagctgttc gatacctact cgaaacaggc
    21481 aggtaaagag cagtatctga tcccgtattt catctctgcg caccccggta cgcgtgatga
    21541 agatatggtg aatttggcgc tgtggctgaa aaagcatcgt ttccgtctcg accaggtaca
    21601 gaacttctac ccatcgccgc tggcgaactc aaccaccatg tattacaccg ggaaaaaacc
    21661 gctggcgaag attggttata agagcgaaga cgtcttcgta ccgaagggcg acaaacagcg
    21721 tcgtttgcat aaagcgttgt tgcgttacca cgatccggca aactggccgt taatccgccc
    21781 ggcgctggaa gcgatgggca aaaagcatct gattggcagc cgtcgcgatt gcttagtgcc
    21841 tgcgccaacc attgaagaga tgcgtgaagc tcgtcgccag aaccgcaata cccgtccggc
    21901 gttgactaaa catacgccga tggcgaccca gcgtcagacg cctgctacgg caaaaaaagc
    21961 gtcgtctacg caatctcgtc cggtgaatgc tggtgcgaag aaacgcccta aagcggcggt
    22021 tggacgttaa aaaaaatccc cggcaactga catgctgccg gggatttttt tatcattctg
    22081 cgtacatacg caagcagcgc aggcaatcaa aatagtcctg agcatcaaga cgcgtattgg
    22141 ctctgttagc ttcggatttg gcagcataga tttgtgggcg tagtgctttt atatcctgct
    22201 caagccgggc gattttggca tttaatgttt gtacattgct cgccagttga ttgacctttt
    22261 gctcattgac gcagccactg agcaagcagg ttgccagaac aacagctccc acggtgaaaa
    22321 tcgttttcat atagtgttcc cttttattta ttgttccggc taattgttat ttaattcagc
    22381 tcattgcttt aatctggcgg cggtttttgc ctcaatctgc ttaagtaggc tgccgctgtt
    22441 cgggcggata aaatcagcga tttcccactt gccttcgcgc cgctgtaaaa ccagttcttg
    22501 ctcttcgttg ttgccattgg tcagcgtaaa acgaacatga atgcgggcgg aatcggcggt
    22561 tttctccagg actcgaattt catcaacgcg tgaaaatagc gactcttcgc caaaacagat
    22621 cggattgtcg taaataaatt gccgttctat ttgcatatct tcatcgggat tattggccgt
    22681 cttgaaggtg gttttataga aggcattaac cgcctggcgg taagagtcgg aaaccaccca
    22741 cgtccacggc cagctataat cagcaatatg ttcaaagagt tcggcaacaa aggcttccgg
    22801 ctgttctttt tgcaacgaag ctaaagcggc cagcgttttt tcattctcgc tattaactgc
    22861 ttgtaagacg ctgccatgat tgctgacaat atcgtcaatg acccaacgac cattttccgc
    22921 caccattttc agtgtctgcg tggtcttttc tttatcatct ttaaagatac gaaagcgcac
    22981 aacggcatcg gcatgatcgg cgtcagtttg ggttatcgca acgctttcta gcaccagatc
    23041 gccaaaatcc tgacaatcac aaaccggatc ataatccagc cagccaatat tgcccggcag
    23101 cgtaagattg tcgttcaggg taagcgcctg ttgaatacgc gctgaagtta tcgcccgctc
    23161 tccggtttca ccgaaataag gtgtactggc atctgattta tagttctgat aaatctgccg
    23221 gactgtttgt tctacggtct gtgaaggagg ctgcgcgtgt acggtaaaac ttgccagggc
    23281 tgctaaaaac agaagtatta ttttcatctg tttttcacct ctccgttgcg tttatcccgg
    23341 atatagatga gttgttcttt taagagcttc cggctctgca tgatgatgtc cttatatttg
    23401 gcattcctga acaaggaaga gtaacaacgg gcgggacgcg aggggaataa atgatttctg
    23461 aaaagtccgg tagcggaaca ttaccgtcac cgggagaatt tgcatgttag ccgccgaact
    23521 ggtcaggatc gggaccgaga cgcttgccct gatcgagttt tgcaatttcg ccgagttcgt
    23581 ctttgtcgag acggaaatcc cagacatcaa agttttcggc aatacgtgaa ggtgtgaccg
    23641 atttcgggat caccaccagg ccgctatcca gatgccagcg gataacaatc tgcgccgggg
    23701 ctttgccgta tttatctgcc agatcgcgaa tgactttctg atcgaaaacg cctttccctc
    23761 cttgcgctaa tgggctccag gattcggtct ggattttgtg tgtcgcgttc caggcgtgta
    23821 gctggcgttg ttgcatcagc ggatgaagtt cgatctggtt tatcacaggc gtcacgccag
    23881 tttcatcaat caggcgttgc aggtgatgga tctggaagtt gcacacgccg atgcttttga
    23941 ttaatccctc tttttgcaat tcgatcatgc ctttccatgc ttcgacataa tggtcgatag
    24001 cgggaacggg ccagtgcatt aagtagaggt cgatataatc aagctggagt tttttcaggc
    24061 tgtcgagcag ggcttcgcgg gggcgcttgt ggtcgtcgtt ccacagctta gtggtgatga
    24121 acagttcttc tctgttgact gaggcatttt tcagggcttt gccgacacct tcttcgttct
    24181 tgtaggccgc ggcggtatca atcgagcgat aacccacttc taacgctttt tgaatggcgg
    24241 tgattacttc ctcattactt gcttgccaga cgcccagtcc cagctggggc atgacattgc
    24301 catcctgtag cttaataacg gttggattag ccatacgttc ctcctttata tgaactcacc
    24361 ggagcaggct ccggtgaggt gttgtgaact taagtctgga cgaaatgccc gaaaacgaaa
    24421 gtttgaggcg taaa
//
LOCUS       DAKISA010000061        24166 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.061, whole genome shotgun sequence.
ACCESSION   DAKISA010000061 DAKISA010000000
VERSION     DAKISA010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24166
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.061"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            237..425
                     /locus_tag="OVW45_002539"
     CDS             237..425
                     /locus_tag="OVW45_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCV5526961.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            437..1189
                     /gene="bcsQ"
                     /locus_tag="OVW45_002540"
     CDS             437..1189
                     /gene="bcsQ"
                     /locus_tag="OVW45_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCV5526962.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            1186..1350
                     /locus_tag="OVW45_002541"
     CDS             1186..1350
                     /locus_tag="OVW45_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCV5526963.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPR"
     gene            1543..3804
                     /gene="bcsA"
                     /locus_tag="OVW45_002542"
     CDS             1543..3804
                     /gene="bcsA"
                     /locus_tag="OVW45_002542"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HCV5526964.1"
                     /translation="MNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSLILA
                     LICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRYTST
                     LNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVDIFV
                     PTYNENLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIARTTHE
                     HAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHFFSP
                     DPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIAVET
                     VTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLDNPL
                     TGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFVLPH
                     MIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGGLVE
                     EEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVLGGA
                     VAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQILEGQ
                     KVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWALWQD
                     SYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRHPERS
                     ETAQPSDQALAQQ"
     gene            3815..6154
                     /gene="bcsB"
                     /locus_tag="OVW45_002543"
     CDS             3815..6154
                     /gene="bcsB"
                     /locus_tag="OVW45_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCV5526965.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKSDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRNVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            6161..7267
                     /gene="bcsZ"
                     /locus_tag="OVW45_002544"
     CDS             6161..7267
                     /gene="bcsZ"
                     /locus_tag="OVW45_002544"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCV5526966.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVIA
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPIGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7249..10741
                     /gene="bcsC"
                     /locus_tag="OVW45_002545"
                     /pseudo
     CDS             7249..10741
                     /gene="bcsC"
                     /locus_tag="OVW45_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            10823..12811
                     /gene="hmsP"
                     /locus_tag="OVW45_002546"
     CDS             10823..12811
                     /gene="hmsP"
                     /locus_tag="OVW45_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCV5526967.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPIMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFAHPLPIEIFEESYLEEK"
     gene            12994..14280
                     /gene="dctA"
                     /locus_tag="OVW45_002547"
     CDS             12994..14280
                     /gene="dctA"
                     /locus_tag="OVW45_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCV5526968.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            14500..15996
                     /locus_tag="OVW45_002548"
     CDS             14500..15996
                     /locus_tag="OVW45_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCV5526969.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNIGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(16092..17021)
                     /gene="kdgK"
                     /locus_tag="OVW45_002549"
     CDS             complement(16092..17021)
                     /gene="kdgK"
                     /locus_tag="OVW45_002549"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCV5526970.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVCLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            17253..18020
                     /gene="pdeH"
                     /locus_tag="OVW45_002550"
                     /pseudo
     CDS             17253..18020
                     /gene="pdeH"
                     /locus_tag="OVW45_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
     gene            18090..20150
                     /locus_tag="OVW45_002551"
     CDS             18090..20150
                     /locus_tag="OVW45_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCV5526971.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPTAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(20332..21654)
                     /locus_tag="OVW45_002552"
     CDS             complement(20332..21654)
                     /locus_tag="OVW45_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCV5526972.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(22065..23078)
                     /gene="yhjD"
                     /locus_tag="OVW45_002553"
     CDS             complement(22065..23078)
                     /gene="yhjD"
                     /locus_tag="OVW45_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCV5526973.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(23127..24026)
                     /locus_tag="OVW45_002554"
     CDS             complement(23127..24026)
                     /locus_tag="OVW45_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCV5526974.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAMVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGQLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
BASE COUNT         5656 a         6183 c         6752 g         5575 t
ORIGIN      
        1 agctaagaaa taaacctacc attgaaatta aaacttatca gatttagctt gtgaatcaat
       61 ttagaaggtc acaagacgtg tgattttagt cttttcgttt ttaacttttt ctgttgcgta
      121 acggaaagtc aaaaagtgag caaattcccg tctcgccgta aacaataaac cggcgaaatg
      181 ctcacggtta gaattgtttc atgaacggta cggttatttc atagggatca agcaaaatga
      241 ataacaatga accagatact ctgcctgatc ccgcgatagg ctatatcttc cagaatgata
      301 ttgtggcgtt aaagcaggca ttttcactgc ctgatattga ttatgccgat atttcccaac
      361 gcgaacagtt ggccgcggca ttaaaacgct ggccgttgct ggcagagttt gcgcaacaaa
      421 agtaggggat tggtgaatgg ccgtactggg attgcagggg gtgcggggag gcgtggggac
      481 aacaaccatc accgccgcat tagcctggtc attacaaatg ttgggagaaa atgtcctggt
      541 ggtcgatgcc tgcccggaca acttgttgcg cctgtcattt aatgttgatt ttacccaccg
      601 tcagggctgg gccagagcga tgctggatgg ccaggactgg cgtgacgctg ggttgcgcta
      661 cacctcgcag ctcgatttgc tgccttttgg tcagttatcc attgaagaac aagaaaatcc
      721 acagcactgg caaacccggc tgagcgatat ttgctccggc ttacagcaac taaaagccag
      781 cgggcgttac cagtggattt taatcgactt accgcgtgat gcctcgcaga taacccacca
      841 gctgctgagt ttgtgcgatc actcgctggc aatcgtcaat gtggatgcca actgccatat
      901 ccgactgcat cagcaagcgc tgccggatgg cgcacatatt ttgattaatg acttccgtat
      961 tggcagtcag gttcaggacg atatttacca gctttggttg caaagccagc gccgattact
     1021 gccgatgctc attcatcgtg atgaagcgat ggctgaatgc ctggcggcta agcaaccagt
     1081 aggtgaatat cgcagtgatg cgctggcggc tgaagagata ctgacgctgg cgaactggtg
     1141 cctgttgaac tactccgggc tgaaaacgcc agtcgggagt gcatcatgag tatcctgacc
     1201 cggtggttgc ttatcccgcc ggtcaacgcg cggcttatcg ggcgttatcg cgattatcgt
     1261 cgtcacggtg cgtcggcttt cagcgcgacg ctcggctgtt tctggatgat cctggcctgg
     1321 atttttattc cgctggagca cccgcgctag cagcgtattc gcgcagaaca taaaaacctg
     1381 tatccgcata tcaacgcctc gcgtccgcgt ccgctggacc cggtccgtta tctcattcaa
     1441 acatgctggt tactgatcgg tacatcgcgc aaagaaacgc cgaaaccgcg caggcgggca
     1501 ttttcaggtc tgcagaatat tcgtggacgt taccatcaat agatgaacga gctgcctgag
     1561 cgcgttagcc ataaaacaca gcatcttgat gagaaaaaag agctcggtca tttgagtgcc
     1621 ggggcgcggc ggttgatcct cggtatcatc gtcaccttct cgctgattct ggcgttaatc
     1681 tgcgttactc aaccgtttaa cccgctggcg cagtttatct tcctgatgct gctgtggggt
     1741 gtagcgctga tcgtacggcg gatgccgggg cgcttctcgg cgctaatgtt gattgtgctg
     1801 tcgctgaccg tttcttgccg ttatatctgg tggcgttaca cctctacgct gaactgggac
     1861 gatccggtca gcctggtgtg cgggcttatt ctgctcttcg ctgaaacgta cgcgtggatt
     1921 gtgctggtgc tcggctactt ccaggtagta tggccgctga atcgtcagcc ggtgccattg
     1981 ccgaaggata tgtcgctgtg gccgtcagtg gatatctttg tcccgactta caacgaaaat
     2041 ctcaatgtgg tgaaaaatac catttacgcc tcgctgggta tcgactggcc gaaagacaaa
     2101 ctgaacatct ggatcctcga tgacggcggc agggaagagt ttcgccagtt tgcgcaaaac
     2161 gtgggggtga aatatatcgc ccgcaccact catgaacatg cgaaagcggg caacatcaac
     2221 aatgcgctga aatatgccaa aggcgagttc gtgtcgattt tcgactgcga ccacgtacca
     2281 acgcgatcgt tcctgcaaat gaccatgggc tggttcctga aagagaaaca gctggcgatg
     2341 atgcagactc cacaccactt cttctcgccg gacccgtttg aacgcaacct gggacgtttt
     2401 cgtaaaacgc cgaacgaagg cacgctgttc tatggtctgg tgcaggatgg caacgatatg
     2461 tgggacgcca ctttcttctg cggttcctgt gcggtgattc gtcgtaagcc gctggatgaa
     2521 attggcggca ttgctgtcga aaccgtgact gaagatgcgc atacttctct gcgtttgcac
     2581 cgtcgtggct atacctccgc gtatatgcgt attccgcagg cggcggggct ggcgaccgaa
     2641 agtctgtcgg cgcatatcgg tcagcgtatt cgctgggcgc gcgggatggt acaaatcttc
     2701 cgtctcgata acccgctcac cggtaaagga ctgaagtttg ctcagcggct gtgctacgtc
     2761 aacgccatgt tccacttctt gtcgggcatt ccacggctga tcttcctgac tgcgccgctg
     2821 gcgttcctgc tgcttcatgc ctacatcatc tatgcgccag cgttgatgat cgccctattc
     2881 gtgctgccgc atatgatcca tgccagcctg accaactcga agatccaggg caaatatcgc
     2941 cactcttttt ggagtgaaat ctacgaaacg gtgctggcgt ggtatatcgc accaccgacg
     3001 ctggtggcgc tgattaaccc gcacaaaggc aaatttaacg tcaccgccaa aggtggactg
     3061 gtggaagaag agtacgtcga ctgggtgatc tcgcggccct acatcttcct tgttctactc
     3121 aacctggtgg gcgttgcggt aggcatctgg cgctacttct atggcccgcc aaccgagatg
     3181 ctcaccgtgg tcgtcagtat ggtgtgggtg ttctacaacc tgattgttct tggcggcgca
     3241 gttgcggtat cggtagaaag caaacaggta cgccgatcgc accgcgtgga gatgacgatg
     3301 cccgcggcaa ttgcccgcga agatggtcac ctcttctcgt gtaccgttca ggatttctcc
     3361 gacggtggtt tggggatcaa gatcaatggt caggcgcaga ttctggaagg gcagaaagtg
     3421 aatctgttgc ttaaacgcgg tcagcaggaa tacgtcttcc cgacccaggt ggcgcgcgtg
     3481 atgggtaatg aagttgggct gaaattaatg ccgctcacca cccagcaaca tatcgatttt
     3541 gtgcagtgta cgtttgcccg tgcggataca tgggcgctct ggcaggacag ctacccggaa
     3601 gataagccgc tggaaagtct gctggatatt ctgaagctcg gcttccgtgg ctaccgccat
     3661 ctggcggagt ttgcgccttc ttcggtgaag ggcatattcc gtgtgctgac ttctctggtt
     3721 tcctgggttg tatcgtttat tccgcgccac ccggagcgga gcgaaacggc acaaccatcg
     3781 gatcaggctt tggctcaaca atgatgataa cgcgatgaaa agaaaactat tctggatttg
     3841 tgcagtggct atggggatga gtgcgttccc ctctttcatg acgcaggcga cgccagcaac
     3901 gcaaccactg atcaatgctg agccagctgt agccgcccag acggaacaaa atccgcaggt
     3961 ggggcaagtg atgccgggcg tgcagggcgc tgatgcgcca gtcgtggcgc agaacggtcc
     4021 ttcgcgtgat gtgaagctga cctttgcgca aattgcgccg ccgccgggca gcatggtgct
     4081 acgtggcatt aacccgaacg gcagcattga gtttggtatg cgcagcgatg aagtggtgac
     4141 gaaggcgatg ctcaacctcg aatacacgcc atcgccatcg ttactgcctg tccagtcgca
     4201 gttaaaggtt tatctcaatg atgagctgat gggcgtgctg ccagtgacca aagaacagtt
     4261 gggtaaaaaa acgctggcgc aaatgcccat taacccactg tttattaccg acttcaaccg
     4321 tgtgcggctg gagtttgtcg gccattatca ggacgtgtgc gaaaacccgg ccagcaccac
     4381 gctttggctg gatgttgggc ggagcagtgg actggatctg acctatcaga ccctgaatgt
     4441 gaagagtgac ctgtcacact tcccggtgcc attctttgac ccgcgcgata accgtactaa
     4501 caccttgccg atggtctttg cgggtgcgcc ggatgttggg ctgcaacaag catctgccat
     4561 tgtcgcctcg tggtttggtt cgcgttctgg ctggcgtggg cagaacttcc cggtgctcta
     4621 taaccaattg ccggatcgca atgccattgt ctttgcaacc aacgacaaac ggccggactt
     4681 cctgcgcgat catccggcgg taaaagcccc ggtgattgag atgattaacc atccgcagaa
     4741 tccttacgtc aaactgctgg tggtgtttgg tcgtgacgac aaagacctgt tgcaggcagc
     4801 gaaaggtatc gctcagggta acattctgtt ccgtggtgaa agcgtggtag tgaatgaagt
     4861 gaaaccgctg ctaccgcgta agccgtacga tgcgccgaac tgggtacgta ccgatcgtcc
     4921 ggtcacattt ggcgaactga aaacctatga agaacagtta caatccagcg gtcttgagcc
     4981 agcagcgatt aacgtttcgc taaacctgcc gccggatctc tacctgatgc gcagtaccgg
     5041 cattgatatg gatattaatt accgctacac catgccgccg gtgaaagaca gttcgcggat
     5101 ggatatcagc ctgaataacc agttcctgca atccttcaac ctgagcagta aacaggaggc
     5161 gaaccgcctg ctgctgcgga ttccggtatt acaaggtttg ctggatggca aaacggatgt
     5221 ctctattccg gcgctgaaac tgggcgcgac caaccagctg cgcttcgact ttgagtatat
     5281 gaacccgatg ccgggcggtt cggtggataa ctgtattacc ttccagccgg tgcagaatca
     5341 tgtggtgatt ggtgacgatt ccaccatcga cttctcgaag tattaccact tcatcccgat
     5401 gccggatcta cgcgcctttg ctaacgcggg cttcccgttc agccggatgg cggatctgtc
     5461 gcaaaccatc accgtgatgc cgaaaacgcc taacgaagca cagatggaaa cgttgctgaa
     5521 tactgttggt tttatcggcg cacagacggg cttcccggcg attaatctga cggtgaccga
     5581 tgatggcagc accattcagg gcaaagatgc cgacatcatg atcatcggtg gtatcccgga
     5641 caaattaaaa gacgataagc agatcgacct gttggtgcag gcgaccgaaa gctgggtgaa
     5701 aacaccgatg cgccagaccc cgttccccgg cattgtgccg gacgagagcg atcgcgcggc
     5761 agaaacccag tcaacgctga cctcttccgg tgcgatggcg gcggtgattg gcttccagtc
     5821 gccgtataac gaccagcgca acgtgattgc gctgctggca gatagcccac gcggttatga
     5881 aatgcttaac gatgcggtga acgatagcgg caaacgcgcc accatgttcg gttcggtcgc
     5941 ggtgatccgc gagtccggta tcaacagcct acgtgttggc gacgtttatt acgtaggtca
     6001 tctgccgtgg ttcgagcgct tgtggtatgc gctggcaaac catccgattc tgctggcggt
     6061 gctggcggca atcagtgtga tattgctggc atgggtactg tggcgtctgc tgcgaattat
     6121 tagtcgtcgt cgtcttaacc cggataacga gtaattgaag atgaatgtgt tgcgtagtgg
     6181 aatcgtgacg atgctgctgt tggctgcctt tagtgttcag gcagcctgta cctggcctgc
     6241 ctgggagcag tttaaaaagg attacatcag tcaggaaggg cgcgtcatcg cccccagcga
     6301 cgcgcgcaaa atcaccacct ccgaagggca aagttacggt atgttctttg ccctggcggc
     6361 taacgaccgt gtagctttcg ataatattct cgactggacg cagaacaatc tcgctcaggg
     6421 ctctttaaaa gaacgtttgc ccgcctggct gtggggcaag aaagagaaca gtaagtggga
     6481 agtgctggac agcaattcgg cctccgatgg tgatgtctgg atggcctggt cgttgctgga
     6541 ggcggggcgt ttgtggaaag agcagcgtta taccgacatc ggcagcgcgt tgctaaaacg
     6601 tatcgcgcgg gaggaagtgg tgacggtgcc tgggctgggt tccatgttgt taccgggcaa
     6661 agtgggtttt gctgaggata acagctggcg ttttaacccc agctacctgc cgccgacgct
     6721 ggcgcagtat ttcacccgct ttggcgcgcc gtggaccacg ctgcgcgaaa ccaatcaacg
     6781 tttattgctg gaaaccgccc cgaaaggttt ttcgccagac tgggtgcgct atgagaaaga
     6841 caaaggctgg cagctaaaag ccgaaaaaac attgatcagc agctacgacg ctatccgcgt
     6901 ttacatgtgg gtaggcatga tgcctgacag cgatccgcaa aaagcgcgga tgctcaaccg
     6961 gtttaaaccg atggcgacat tcactgagaa aaacggttat ccgccggaaa aagtggatgt
     7021 ggctacgggg aaagcgcagg gtaaaggacc gatcggtttt tctgccgcca tgctgccctt
     7081 tttacaaaac cgcgatgcgc aggccgttca gcgccagcgc gtggccgata actttcccgg
     7141 cagcgatgcc tattacaact atgtgctgac cctgtttgga caaggctggg atcaacaccg
     7201 tttccgcttc tcgacaaaag gtgagttatt acctgactgg ggccaggaat gcgcaaattc
     7261 acactaaaca tattcacgct ttccctcggt ctggccgtca tgccgatggt cgaggcagca
     7321 ccaaccgctc agcaacagtt gctggagcaa gttcggttag gcgaagcgac ccatcgtgaa
     7381 gatctggtgc aacagtcgtt atatcggctg gaacttattg atccgaataa cccggacgtc
     7441 gttgccgccc gtttccgttc tttgttacgt cagggcgata ttgatggcgc gcaaaaacag
     7501 ctcgatcggc tgtcgcagtt agcgccgagt tcaaatgcgt ataaatcgtc gcggactacg
     7561 atgctacttt ccacgccgga tggtcgtcag gcactgcaac aggcacgatt gcaggcgacg
     7621 accggtcatg cagaagaagc tgtggcgagt tacaacaaac tattcaacgg tgcgccgccg
     7681 gaaggtgaca ttgctgtcga gtactggagt acggtggcga aaattccggc tcgccgtggc
     7741 gaagcgatta atcagttaaa acgcatcaat gcggatgcac cgggcaatac gggcctgcaa
     7801 aacaatctgg cgctattgct gtttagtagc gatcgccgtg acgaaggttt tgccgtcctg
     7861 gaacagatgg caaaatcgaa cgccgggcgc gaaggggcct ctaaaatctg gtacgggcag
     7921 attaaagaca tgcccgtcag tgatgccagt gtgtcggcgc tgaaaaaata tctctcgatc
     7981 tttagtgatg gcgatagcgt ggcggctgcg caatcgcaac tggcagaaca gcaaaaacag
     8041 ctggccgatc ctgctttccg cgctcgtgcg caaggtttag cggcggtgga ctctggtatg
     8101 gcgggtaaag ccattcccga actacaacag gcggtgcggg cgaacccgaa agacagtgaa
     8161 gctctggggg cgctgggcca ggcgtattct cagaaaggcg atcgcgccaa tgcagtggcg
     8221 aatctggaaa aagccctcgc actggacccg cacagcagca acaacgacaa atggaacagt
     8281 ctgctgaaag taaaccgcta ctggctggcg atccagcagg gcgatgctgc gctgaaagcc
     8341 aataatcctg accgggcaga acgcctgttc cagcaggcgc gtaatgtcga taacaccgac
     8401 agttatgcag tgctggggct gggcgatgtg gcgatggcgc gaaaagatta tcccgccgcc
     8461 gaacgttatt atcagcagac cttgcgtatg gacagcggca acactaacgc cgtgcgcggg
     8521 ctggcaaata tttaccgcca gcaatcgcca gaaaaagctg aagcgtttat cgcctcgctc
     8581 tctgccagtc agcggcgtag cattgatgat atcgaacgca gcctgcaaaa cgaccgtctg
     8641 gcacagcagg cagaggcact ggaaaaccag ggcaaatggg cgcaggcggc agcacttcag
     8701 cggcaacgac tggcgctgga ccccggcagc gtatggatta cttaccgact ttcgcaggat
     8761 ctctggcagg ccggacaacg cagccaggcc gatacgttaa tgcgcaatct ggcgcagcag
     8821 aagtcgaacg acccagagca ggtttacgct tacgggctgt acctacgctt acgggctgta
     8881 cctctctggt catgaccagg acagagcggc gctggcgcat atcaacagcc tgccgcgtgc
     8941 gcagtggaac agcaatattc aggagctggt taatcgactg caaagcgatc aggtgctgga
     9001 aaccgctaac cgcctgcgag aaagcggcaa agaggcagaa gcggaagcga tgctgcgcca
     9061 gcaaccacct tccacgcgta ttgacctcac gctggctgac tgggcgcaac aacgacgtga
     9121 ttacaccgcc gcccgcgctg catatcagaa tgtcctgacg cgggagccag ctaacgccga
     9181 cgccattctt ggtctgacgg aagtggatat tgctgccggt gacaaagcgg cggcacgtag
     9241 ccagctggcg aaactgcccg ctaccgataa cgcctcgctg aacacacagc ggcgcgtggc
     9301 gctggcacag gcgcagcttg gcgataccgc agcagcgcag cggacgttta ataagttgat
     9361 cccgcaggca aaatctcagc caccgtcgat ggaaagcgcg atggtgctgc gtgatggtgc
     9421 gaagtttgaa gcgcaggcgg gcgatccaac gcaggcgctg gaaacctaca aagacgccat
     9481 ggtcgcatcc ggtgtgacta cgacgcgtcc gcaggataac gacaccttta cccgactgac
     9541 ccgtaacgac gagaaagatg actggctgaa acgtggcgtg cgcagcgatg cggcggacct
     9601 ctatcgccag caggatctta acgtcaccct tgagcacgat tactggggtt cgagcggcac
     9661 cggtggttac tccgatctga aagcgcacac taccatgttg caggtggatg cgccgtattc
     9721 tgacgggcgg atgttctttc gcagtgattt cgtcaatatg aacgtcggca gtttctccac
     9781 taatgccgat ggcaaatggg atgacaactg gggcacctgt acattacagg actgtagcgg
     9841 caaccgcagc cagtcggatt ccggtgccag cgtggcggtc ggctggcgaa atgacgtctg
     9901 gagctgggat atcggtacca cgccgatggg cttcaacgtg gtggatgtgg tcggcggcat
     9961 cagttacagc gatgatatcg ggccgctggg ttacaccgtt aacgcccacc gtcggcccat
    10021 ctccagttct ttgctggcct ttggtgggca aaaagactcc ccgagcaata ccgggaaaaa
    10081 atggggtggc gtacgtgccg acggtgtggg gctaagtctg agctacgata aaggtgaagc
    10141 aaacggcgtc tgggcatcgc ttagtggcga ccagttaacc ggtaaaaatg tcgaagataa
    10201 ctggcgcgtg cgctggatga cgggctatta ctataaggtc attaaccaga acaatcgccg
    10261 cgtcacaatc ggcctgaaca acatgatctg gcattacgac aaagatctga gtggctactc
    10321 actcggtcag ggcggttact acagtccgca ggaatacctg tcgtttgcca taccggtgat
    10381 gtggcgggag cgcacggaaa actggtcgtg ggagctgggg gcgtctggct cgtggtcgca
    10441 ttcacgcacc aaaaccatgc cgcgttatcc gctgatgaat ctgatcccga ccgactggca
    10501 ggaagaagct gcgcggcaat ccaacgatgg cggcagcagt cagggcttcg gctacacggc
    10561 gcgggcatta cttgaacgac gtgttacttc caactggttt gttggcacgg caattgatat
    10621 ccagcaggcg aaagattacg cacccagcca tttcctgctc tacgtacgtt attccgccgc
    10681 cggatggcag ggtgacatgg atttaccgcc gcagccgctg ataccttacg ccgactggta
    10741 agttttcaga tagcgcctct cttaatgccg ctgcgatcgg gtatactcgg gcggcaatct
    10801 gggatttccg gggggagaca atttgcgcgt aagtcgctcg ttaacaatca agcagatggc
    10861 aatggtggca gccgttgtcc tggtgttcgt ttttattttt tgcaccgttt tgctgttcca
    10921 tctggtccag cagaatcgct ataacacggc tacgcaactg gaaagcattg ctcgctctgt
    10981 ccgcgaaccc ttatcttcag ctattttgaa aggcgatatt cccgaagcgg aagctattct
    11041 tgccagcatt aaaccggcag gcgtggtcag ccgtgccgat gtagtgctgc ctaaccagtt
    11101 ccaggcgctg cgtaaaagtt ttattccaga gcgcccggtg ccgataatgg ttactcgcct
    11161 gtttgagcta ccggttcaaa tctcgctggg cgtttactcg ctcgaacgtc cggcaaaccc
    11221 gcagccaatt gcctatctgg tactacaggc ggattccttc cgtatgtata agttcgtgat
    11281 gagcaccctc tcaacgttag tgaccattta cttacttttg tcgcttatcc tgaccgtcgc
    11341 catcagctgg tgcattaacc gcctgatttt gcatccgtta cgcaatattg ctcgcgaact
    11401 taacgccatc ccagcccagg agcttgttgg tcaccaactg gcattaccgc gtctgcatca
    11461 ggacgatgaa atcggtatgt tggtgcgcag ttacaacctc aaccagcaat tgctgcagcg
    11521 ccattatgaa gaacagaacg aaaatgcgat gcgcttcccg gtgtcggatt tgccgaacaa
    11581 agccttgctg atggagatgc tggagcaggt tgtcgcgcgt aaacaaacca ccgcgctgat
    11641 gatcatcacc tgtgaaaccc tgcgtgatac tgcgggcgtg ctgaaagagg cgcaacgaga
    11701 aattctgctg ctgacgctgg tggaaaaact caaatcggta ctgtcgccac gtatgatcct
    11761 cgcgcagatt agcggttatg actttgctgt cattgccaac ggtgtacagg aaccgtggca
    11821 cgcaatcacc ttaggtcagc aagtgctcac tatcatgagc gagcgcctgc cgattgaacg
    11881 tattcaactc cgtccgcact gtagcattgg cgtggcgatg ttctacggcg atctcaccgc
    11941 cgaacagctt tacagtcgcg ctatttctgc ggcatttacc gctcgccata aaggcaagaa
    12001 tcagattcag ttctttgatc cgcagcagat ggaagccgcc cagaagcggt tgacggaaga
    12061 gagcgatatc cttaatgcac tggaaaatca tcagtttgct atttggttac agccacaggt
    12121 cgagatgacc agcggtaaac tggtcagtgc ggaagtgtta ctgcgtatcc agcaaccgga
    12181 tggcagttgg gacctgccgg atggcttaat cgatcgcatt gagtgctgtg ggctgatggt
    12241 taccgtcggt cactgggtgc tggaagagtc ctgtcgattg cttgcagcct ggcaagagcg
    12301 cggcattatg ctgcccttgt cggtaaacct ctctgcgctg caactgatgc acccaaatat
    12361 ggtggcggat atgctggaac tgttaacccg ctatcgcatt cagccgggaa cactgattct
    12421 ggaagtgaca gaaagccgac gtattgacga ccctcatgct gcggtggcaa tcctccgtcc
    12481 gctgcgcaat gccggagttc gggtggcgct ggatgatttc ggcatgggct acgcagggct
    12541 gcgtcagctg cagcatatga aatcgttgcc aatcgacgta ctgaaaatcg acaaaatgtt
    12601 tgttgaaggc ttgccggaag atagcagcat gattgctgca attatcatgc tggcgcagag
    12661 cctgaactta caaatgattg ccgaaggcgt ggagactgaa gcacaacgcg actggctggc
    12721 aaaagcgggc gttggtattg cccagggctt cctttttgct cacccactcc ctattgaaat
    12781 cttcgaagag agttacctgg aagaaaagta gctaccccaa actgattaca aaactttaaa
    12841 aagtgctggt ttgtgcgagc cagctcaaac tttttaacct ttttgtttca attatgatcc
    12901 aggtacattt ctgtgatgtt gtctgggtgt tattttaagg ccgcaggtac cccataacct
    12961 tacaagacct gtggttttac taaaggacac cctatgaaaa cctctctgtt taaaagcctt
    13021 tactttcagg tcctgacagc gatagccatt ggtattctcc ttggccattt ctatcctgaa
    13081 ataggcgagc aaatgaaacc gcttggcgac ggcttcgtta agctcattaa gatgatcatc
    13141 gctcctgtca tcttttgtac cgtcgtaacg ggcattgcgg gcatggaaag catgaaggcg
    13201 gtcggtcgta ccggcgcagt cgcactgctt tactttgaaa ttgtcagtac catcgcgctg
    13261 attattggtc ttatcatcgt taacgtcgtg cagcctggtg ccggaatgaa cgtcgatccg
    13321 gcaacgcttg atgcgaaagc ggtagcggtt tacgccgatc aggcgaaaga ccagggcatt
    13381 gtcgccttca ttatggatgt catcccggcg agcgtcattg gcgcatttgc cagcggtaac
    13441 attctgcagg tgctgctgtt tgccgtactg tttggttttg cgctccaccg tctgggcagc
    13501 aaaggccaac tgatttttaa cgtcatcgaa agtttctcgc aggtcatctt cggcatcatc
    13561 aatatgatca tgcgtctggc acctattggt gcgttcgggg caatggcgtt taccatcggt
    13621 aaatacggcg tcggcacact ggtgcaactg gggcagctga ttatctgttt ctatattacc
    13681 tgtatcctgt ttgtggtgct ggtattgggt tcaatcgcta aagcgactgg tttcagtatc
    13741 ttcaaattta tccgctacat ccgtgaagaa ctgctgattg tactggggac ttcatcttcc
    13801 gagtcggcgc tgccgcgtat gctcgacaag atggagaaac tcggctgccg caaatcggtg
    13861 gtggggctgg tcatcccgac aggctactcg tttaaccttg atggcacatc gatatacctg
    13921 acaatggcgg cggtgtttat cgcccaggcc actaacagtc agatggatat cgtccaccaa
    13981 atcacgctgt taatcgtgtt gctgctttct tctaaagggg cggcaggggt aacgggtagt
    14041 ggctttatcg tgctggcggc gacgctctct gcggtgggcc atttgccggt agcgggtctg
    14101 gcgctgatcc tcggtatcga ccgctttatg tcagaagctc gtgcgctgac taacctggtc
    14161 ggtaacggcg tagcgaccat tgtcgttgct aagtgggtga aagaactgga ccacaaaaaa
    14221 ctggacgatg tgctgaataa tcgtgcgccg gatggcaaaa cgcacgaatt atcctcttaa
    14281 tctcaccact tatgcccgca ctcccttccc cgagtgcggg tgtttgcgca taattgaccc
    14341 ctgtgcctga acttaccacc tgggtgagtt caggtttatt tcacttcttc cgtgacattt
    14401 tcatgttctt gcggtctaac acgaagtgtt tttaacgtca tttagactga ctgacaacgg
    14461 gcagtttttt attaccagga tagttgatca ggggttcaca tgcagggcac aaaaattcga
    14521 cttttagcgg gcggtttgct gatgatggcc actgctggct atgtgcaggc agatgcgctc
    14581 cagcctgatc cagcatggca acaggggacg ctttccaacg gtttacagtg gcaagtgctg
    14641 accacccccc agcgtcccag cgatcgtgtt gaaattcgcc tgctggttaa tatcggttcg
    14701 ctcgccgaaa gtacacaaca gagcggttac agtcacgcca tccctcgtat tgcgctaacg
    14761 caaagcggtg gccttgacgc agcacaggcg cgttcattgt ggcagcaggg gatcgaccct
    14821 aaacgcccga tgccgccggt aattgtctct tatgacacca cgctgtttaa tctgagtttg
    14881 cccaataacc gtaacgattt gctgaaagaa gcgctctctt atctggcaaa tgccactggc
    14941 aaattgacca tcacaccaga aaccatcaac cacgcgctgc aaagtcagga catggtggca
    15001 acctggcctg ccgatactaa agagggctgg tggcgctatc gtctgaaagg gtcaaccttg
    15061 ttaggtcacg atcctgccga tccgctgaaa caacccgttg aagcggaaaa aattaaagat
    15121 ttctatcaga aatggtacac cccggatgca atgacgctac tggtggtggg aaacgtggat
    15181 gcgcgctcgg ttgtcgacca aatcaacaaa acgtttggcg aactgaaagg caaacgtgaa
    15241 acgccagctc cggtgccgac gctttctccg ctgcgtgcgg aagcggtgag tattatgact
    15301 gacgcggtgc gtcaggaccg gttatctatc atgtgggata cgccgtggca gccgattcgt
    15361 gaatcagccg cactgctgcg ctactggcgt gcggacctgg cccgcgaggc gctgttctgg
    15421 catgttcagc aagcgttaag cgccagtaac agcaaagaca tcggtcttgg atttgactgc
    15481 cgtgtgctgt atctgcgtgc gcagtgtgcc atcaacatcg aatcaccaaa cgacaagctg
    15541 aacagcaacc ttaatctggt ggcgcgtgaa ctggcgaagg ttcgcgataa aggtctgccg
    15601 gaagaagagt tcaatgcgtt agtggcgcaa aagaaactgg agctgcagaa actgtttgcc
    15661 gcctatgcgc gggctgatac cgatattctg atgggtcagc ggatgcgttc gctgcaaaat
    15721 caggtcgtcg atatcgcgcc ggagcagtat cagaaactgc ggcaggattt ccttaacagc
    15781 ctgacggtgg agatgttaaa tcaggatctg cgtcagcagt tgtcgaatga tatggcgtta
    15841 atactgctgc agccgaaagg cgagccggaa tttaacatga aagcgttgca ggcggcctgg
    15901 gatcaaatca tggccccatc gactgctgct gccgccacct ctgtcgccac ggatgacgta
    15961 catcctgaag tgacggatat tccacctgca cagtaatgcc tgatggtgat gagttagcac
    16021 aaccatcagg tatttatgga tgagctggat agaggggtaa cactttatcc agccttttgc
    16081 atatgctgcg tttacgctgg catcgcctca cgcgggataa tcgcgccgcg atactgaata
    16141 acggtacttg cggtcaggtg cccgcgtttc gccgcgtttt ccgcactgcc gcccgtcaaa
    16201 cataccgcca gataaccggc gctgaaagag tcgccagctg cggtggtatc gatcactttt
    16261 tctttcggca gtttcaccgc cggaacatcc actaaccctt cgccagcaat ggacaccagg
    16321 caagaatccg ccccgcgttt caccaccact tctttcacgc ccgcgttatg ggtgcgcgca
    16381 atgacgtctt ccaccggctg ttgaccccac agcgcgtctt catcgtccag cgtcaggaaa
    16441 gcgatatccg tgcattccag catttgttgg tacacctgct gtgtctcttc tttgctggcc
    16501 cacaggcgcg gacgatagtt attgtcgaaa atcacttttc cgccgttggc gcggcattcg
    16561 cgcagcaggg aaagcagctt ttcgcggctg gtcgggctta agatcgccag gctaatcccg
    16621 ctcaggtaga gataatcgaa attcgccagc tcttcgcaaa tcgccgcaga ctgctcactc
    16681 tccagccaga atttggcggc ggcttcgttc cgccagtagt agaacgtacg ctcgccggtg
    16741 ctgtcggttt caatgtagta aaggcccggc agacggtttt ccatccgttg ggtcagggaa
    16801 gtatcaacgt tctcgccgtg ccaggcgtcc agcatttgct ggctaaaact gtccgttccc
    16861 agcgccgtta cgtaatgaac ggttaatgcc gcaggatcga cctgacgggc gatatagacg
    16921 gaagtgttca gggtatcgcc gccgaaaccg cgcttaacgt ccgcgccttt ctcggaaagc
    16981 tcaatcatgc attcgccaat cacggcaatc tttttggaca tagtcgtgaa cctgatctgt
    17041 aaaaaattag cgttagtttg cgctgtggtg gtttgctggt caaccatatt aaaacagtgt
    17101 tccattattt ttttgagcca gaacgtattc ctgaaagatt ccgttgtgga gtgaggaaat
    17161 ttaccccggc caggcggtaa agttctgccc ttgcgcgccg ataatctttg tcgagtccgg
    17221 gcagcatcac ttttaaacac aggacatctt tgatgataag gcaggttatc cagcgaataa
    17281 gcaaccctga agcaagcatc gagagcttgc aggaacggcg tttttggttg cagtgtgagc
    17341 gtgcttacac ctggcagccg atctatcaaa catgcggacg gttaatggcc gtggagctat
    17401 taacggtggt cacgcatccc ttgaaccctt cgcaacgcct gccgccggat cgctatttta
    17461 ctgaaatcac cgtcagccat cggatggagg ttgtgaaaga gcagattgat ttgctggcgc
    17521 aaaaagccga cttctttgta gagcacgacc tgctggcatc ggtcaatatt gatggcccta
    17581 cgctcatcgc cctgcgtcag caaccaaaaa tcctgcgcca aattgagcgt cttccctagc
    17641 tgcgtttcga actggtggaa cacatccgcc tgccgaaaga ttcaaccttt gcctcgatgt
    17701 gtgaatttgg cccgctgtgg ctggatgatt ttggcaccgg gatggcaaat ttctctgcgc
    17761 taagtgaagt gcgttatgac tacatcaaaa tcgcgcgaga gctgttcgtg atgctgcgtc
    17821 agtcgccgga aggccgcaca ctcttttctc agcttttaca tctaatgaat cgctattgtc
    17881 gcggggtgat tgtcgagggc gtagaaacac cggaagagtg gcgtgatgtt cagaactcgc
    17941 ccgcattcgc cgcacaaggc tggtttcttt cacgcccggc accgatggaa acgctgaata
    18001 cggcggttct ggcgctataa gctgcctcat tttccgctgg gctggactat ctttaggact
    18061 ggcacaggaa gaatatgagg aagcgaacga tgagcaaggc aggcaaaata accgctgcga
    18121 tttcaggggc tttcttgttg ttgattgtcg tggcgatcat tttgattgca acttttgact
    18181 ggaatcgact caaaccgacc atcaaccaga aagtctctgc ggagttgaat cgtccgttcg
    18241 ctatccgtgg cgatctgggc gtggtgtggg agcggcaaaa acaagaaact ggctggcgca
    18301 gctgggtgcc gtggcctcat gtacatgcag aagacatcat tcttggtaat ccaccggata
    18361 ttcccgaagt cacgatggtg catttgccgc gagtagaggc aacgctggca ccgctggcgc
    18421 tgctgaccaa aacggtctgg ctgccgtgga tcaagctcga aaagcccgac gcgcgcctga
    18481 ttcgcctctc cgaaaagaac aataactgga cgtttaatct tgccaacgat gacaacaaag
    18541 acgcgaatgc aaagccgtcg gcatggtcgt ttcggctgga taatattctt ttcgatcaag
    18601 ggcggatcgc cattgatgac aaagtaagca aagcggatct ggaaattttt gttgatccgt
    18661 taggcaagcc gctgccgttc agcgaagtta ctggatcgaa aggtaaagcg gataaagaaa
    18721 aggtgggcga ttacgttttt ggcctgaagg cgcagggacg ttataacggt gaaccgctca
    18781 cgggtacggg aaaaataggc ggtatgctgg cgctgcgtgg cgaagggacg ccgtttccgg
    18841 tacaggctga tttccgttca ggtaataccc gtgttgcttt tgatggcgtc gtgaatgacc
    18901 caatgaagat gggcggtgtc gatttacggc ttaaattttc tggcgattca ctgggtgatc
    18961 tctatgaact gacgggcgtt ctgctgcccg ataccccgcc gtttgaaacg gatggtcggc
    19021 tggtagcgaa aatcgacact gaaaaatcgt cggtctttga ttatcgcggt tttaatgggc
    19081 gaattggcga tagcgatatc cacggttctc tggtctacac caccggaaag ccacgaccaa
    19141 aactggaagg tgatgtcgag tcgcggcaat tgcggctggc ggacctggga ccgttgattg
    19201 gcgttgattc cgggaaaggg gcagaaaagt cgaaacggtc tgaacagaag aagggcgaaa
    19261 aaagcgttca gcctgcgggc aaagtgctgc cttatgaccg cttcgaaacc gataaatggg
    19321 acgttatgga tgccgatgtt cgcttcaaag ggcggcgcat tgagcatggc agtagcctgc
    19381 cgattagcga tctttctact catatcatcc tcaaaaatgc tgacctgcgc ctgcaaccgc
    19441 tgaaatttgg catggcgggc ggcagcattg cggcgaatat tcatctggaa ggcgataaaa
    19501 agccgatgca ggggcgggca gatattcagg ctcgtcgact gaaactgaaa gaactgatgc
    19561 ccgatgtgga actgatgcag aagacgctgg gggaaatgaa cggtgacgcg gaactacgcg
    19621 gtagcggtaa ctcggtggcg gcgcttttag gcaacagtaa cggcaacctg aaactgttga
    19681 tgaatgacgg gctggtgagc cgcaacctga tggagattgt tgggctgaat gtcggcaact
    19741 acattgtcgg tgcgatattt ggtgacgatg aggtgcgggt gaactgcgcg gcggcgaatc
    19801 tgaatattgc caacggcgtg gcgcgcccgc agatttttgc tttcgatact gagaacgcgt
    19861 tgattaatgt taccggcacg gcaagttttg cttcagaaca gctggatttg actattgatc
    19921 cggagagtaa agggattcgg attatcacac tgcgttcgcc gctgtatgtg cgtgggacgt
    19981 ttaaaaatcc gcaggctggg gtgaaagccg gaccgctgat tgcccgtggt gctgttgctg
    20041 cggcactggc aacgctggta acaccgacgg cggcgttact ggcactgatc tcaccttccg
    20101 aaggggaggc taatcagtgt cggacgatat tgtcgcagat gaagaagtga tgataattgc
    20161 cggatgtctg acatccggca attaaacaga tttagatttt tggcaaagtg tgctttgttc
    20221 atgccggatg cggcgtgaac gccttatccg gcctacaaat ccttgtgaat tcaatatatt
    20281 gtatggaaat tcagtcctga taagcgtagc gcatcaggct tttcacactt attacaacga
    20341 ctgatgtcgc gtctcatggg tcagcagcag ggcgattaac gtcaagccag ccatcgccgc
    20401 cagatataac cccaccgcac ctaacccgta gttagtctgc aaccaggctg cgatatacgg
    20461 tgcaacggaa gccccgagaa tcgacgctac gttgtaagag aatgatgctc cggtgtaacg
    20521 cacttctgtt gggaacagct ctggtaacag cgcgcccatc ggcccgaagg tcagccccat
    20581 cagacttaac cccagcagca ggaaggcaaa aaccagaatc gggttgccag aaccgagcag
    20641 cgggttaaag gcgaacagcg cgaacaggat gatcagcgtg gtgataatta ccatgctttt
    20701 acggcgacca aaggcatcag ccagtaatcc ggcgactggc accatcacgc caaaaccaat
    20761 aactgccatc atcagcatcc acaacacttc gttacgcggc aggccaagcc caactggcgc
    20821 ggcggcggta ctgaaggtca tagagtagac cgtcatgatg taaaacagcg tataggttgc
    20881 cagcatgatg aacgtaccca gtacggttac gcgaacatgt ttggtcagca gcgtacccag
    20941 cgggatcttc acctgttttt tcgctttagc gactttctca aacaccggcg actcatgcag
    21001 cgacacgcga acatacaggc cgataatgac cagtaccgcc gagaagataa atggcacacg
    21061 ccagccccag ctcataaact gctcgtcggt cagtagccag gaaagcagca agaaagtgcc
    21121 gttggcaaag aagaagccaa tcggtgcgcc cagctgtggg aaggagccat acagtgcacg
    21181 tttgcgcggt ggggcgtttt cagtcgccag cagcgccgcg ccgccccatt caccgcctaa
    21241 gcccagaccc tgaccaaatc gagccagcgc cagcagcagc ggggcgaaaa taccaatcgt
    21301 ggcatagccc ggcagcagac caatcaccac ggttgaaatc cccatcgtta gcaacgaggc
    21361 gaccagcgtc gctttacgcc caacgcgatc gccaaaatga ccaaaaacgg cagagccaat
    21421 ggggcgcgcg acgaaggcga tggcgaaggt ggcgagcgac tgtagcgttg ctgccgttgg
    21481 atcgccctgc gggaagaaga tatgcggaaa cacaataacg gccgcagtgg cgtaaatgta
    21541 aaagtcgaag aactcaatgg ccgtgccaat gagagaggcg acaaggactt tattacgcga
    21601 gttgatcggc gtgtattctt gctcgtggtc gagtgttgtg gctgttgctt gcataatctt
    21661 ttcttatttt ttggctaacg aatagccata ttacgcacag caaaagtgat atttcaatgt
    21721 gtaacaaatc gcggaaatgg tcaaaaacca ggcaaaaagg ggataatgtt taggccagtg
    21781 atttttcttc tatgaaatgc ttcacataat tttataaata gtggataaaa ctggtttcag
    21841 gttaaataaa agttaccaac tggatttata tgctggtgtt atgagttgga atgaaattgt
    21901 ttgctcccaa atagatggct gaagaggctc tggggaagag tttcaagagg ttatgtgcgc
    21961 attatagccg gatgcgatgc tggcgcatct tatccggcct acggggagcc aggccgaata
    22021 aaatcgtatg ccggataagg cgtttacgcc gcatccggcc tattttaagg ctgcgttttc
    22081 cctggcattc gcgggtcgtc tttatattcg gcggtggcaa tccacgccgc gcaaaacagc
    22141 gtcaaacggg cgaagaagta gaaaaatgcc atcagcccca gcacggaacc aaatgctgcg
    22201 ccagaggggg atttcatcag cgatggcagg gtgtaggtca tcacgatttt aatcacttca
    22261 aaaccaatag cagccagaaa tgttccgcga atcagggctt ttttgcgcgg acggtggcgc
    22321 ggtaaccgcc agaagatcca gaagaaaagc agatagttgg cgaagatgga aatcgccagg
    22381 ccaatcaacc gccacgtcgg tttcagccac tcaatgctgt tcaggtgcag ggcgctaata
    22441 atcatttgct gcgccgaacc ggcaaccgag gtgatcgaaa gcgtcacaat cagcgcaatc
    22501 aacaaaccaa tcagcgaaat aaaatcacgt agatatttta cccagaactt ctcctgatct
    22561 tgcggcgagc gttcccagac atcgcgcgac tgggcgcgaa tcgcttcacg caggttaccc
    22621 atccagttga tgccggaata aagcgccaca gccaggccga caagccctac agtcgtacgc
    22681 tgctgaacgg cggtgttgat ggtgtttttc aacgtggcgg ctagcgtcgg atcgctgatg
    22741 ttttgcagaa ttttgtcgaa gatatcctgt agcaacatcg gatgggaggc cagcacaaag
    22801 ccccctgcgg caaacgacac catcaaaatc gggatcatcg acaaaaatga gaaataggtg
    22861 atagccgcac caaactggtt acccagccga tcgttaaagc gttctgtcgc gcgaatcagg
    22921 tgcgcaataa ccggttggcg ttggactttt tcggcagtgg tggtgacggt ttccagcgcc
    22981 tggctaactt tgccgccttt ctcgctatta tccaactgct taatcggctc gtgctccaga
    23041 tcctgggtgg gacgtttgat ctcgttttcc tgcgtcatcc gctcttttgt ccttctgttg
    23101 tggtcattct taaaaagtat agtcggtcag tccaggtact ctttcattac gccagccagc
    23161 cactgcataa acaggtttac acgccgggaa agctcccgac gctgcggata aaccagcgaa
    23221 agggagagcg gctcggcacg gtagcctggc aacacttcaa taagctgccc ggcacgcagg
    23281 gcttcgcgca cggcaatgcg cgggatctga ataatcccca gaccggcaag accggcggcg
    23341 agatagtttt cgctactgtt taccgtcaac atgccgccag acttaaacca ctggacgcca
    23401 ttaacgctgg caacctcaaa acctaacgga tgtacaccca ggtgcggtgt ataacgcacc
    23461 attgcgtgtg aagtcagatc atcggggctt tgcggataac caaagcgcgt cagatagtgc
    23521 ggactggcac agttgaccat ggtcagtttg ccgaggggac gggcgataac gccatcttcc
    23581 ggtaacgcgc cagtgcgtat cacgcaatca aaaccatcat gaagaatatc taccggacgg
    23641 ttatggctac tcagttccag ctcaattccc ggatgctgat agagaaattc cgacagacgc
    23701 ggcagtaaca ggtttttcgc gattcctgac gggatgtcga tgcgtaattt accgctgata
    23761 ctggtggcat cctgttgaaa cagaccgtcc agttcgctga gattactcaa cacatctttt
    23821 gcccgttgat aataggtcat gccttctggc gtgagtttga cccgtcgcgt agtgcgctgg
    23881 agaagctggg tgccaagttg atgttccagt gcctgtatct ggcgcgaaac acttcccttt
    23941 ggcaaagcaa agaaatccgc cgcgcgggaa aaactttcca gctccgcgac tttgatgaac
    24001 aactgcattg cgtgaatttt atccatttct ttgaccgatt gttgttcaca gcgaaacagt
    24061 aacgcgcata tcgcattgtt tattgttttt tctaacatct aatgccgggt ttcacaatgt
    24121 tgtcatgacg aatgtggcga tttctcagca ggaaaagggc ggaata
//
LOCUS       DAKISA010000062        23954 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.062, whole genome shotgun sequence.
ACCESSION   DAKISA010000062 DAKISA010000000
VERSION     DAKISA010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23954
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.062"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(39..401)
                     /locus_tag="OVW45_002555"
     CDS             complement(39..401)
                     /locus_tag="OVW45_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCV5526975.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            938..2851
                     /gene="mtlA"
                     /locus_tag="OVW45_002556"
     CDS             938..2851
                     /gene="mtlA"
                     /locus_tag="OVW45_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCV5526976.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAVTRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            3081..4229
                     /gene="mtlD"
                     /locus_tag="OVW45_002557"
     CDS             3081..4229
                     /gene="mtlD"
                     /locus_tag="OVW45_002557"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCV5526977.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            4229..4816
                     /gene="mtlR"
                     /locus_tag="OVW45_002558"
     CDS             4229..4816
                     /gene="mtlR"
                     /locus_tag="OVW45_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCV5526978.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(4828..5037)
                     /locus_tag="OVW45_002559"
     CDS             complement(4828..5037)
                     /locus_tag="OVW45_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5526979.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            5322..5684
                     /locus_tag="OVW45_002560"
     CDS             5322..5684
                     /locus_tag="OVW45_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCV5526980.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            6227..6905
                     /locus_tag="OVW45_002561"
                     /pseudo
     CDS             6227..6905
                     /locus_tag="OVW45_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
     gene            6949..11799
                     /locus_tag="OVW45_002562"
     CDS             6949..11799
                     /locus_tag="OVW45_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HCV5526981.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDSWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTASIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIEENGAGINYVRTN
                     DNDLAFNDASASGVGATAVGYNAVASGASSVAIGQNSSSTVDTGIALGSSSVSSRVIA
                     KGSRDTSVTENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTVVNGNAGIGIGLNTLVLADAI
                     NGIAIGSNARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEFSVGSEDGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDERVAQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            12167..13822
                     /gene="lldP"
                     /locus_tag="OVW45_002563"
     CDS             12167..13822
                     /gene="lldP"
                     /locus_tag="OVW45_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCV5526982.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQIDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            13822..14598
                     /gene="lldR"
                     /locus_tag="OVW45_002564"
     CDS             13822..14598
                     /gene="lldR"
                     /locus_tag="OVW45_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCV5526983.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            14595..15785
                     /gene="lldD"
                     /locus_tag="OVW45_002565"
     CDS             14595..15785
                     /gene="lldD"
                     /locus_tag="OVW45_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCV5526984.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            15981..16454
                     /gene="trmL"
                     /locus_tag="OVW45_002566"
     CDS             15981..16454
                     /gene="trmL"
                     /locus_tag="OVW45_002566"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCV5526985.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(16507..17328)
                     /gene="cysE"
                     /locus_tag="OVW45_002567"
     CDS             complement(16507..17328)
                     /gene="cysE"
                     /locus_tag="OVW45_002567"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCV5526986.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(17408..18427)
                     /gene="gpsA"
                     /locus_tag="OVW45_002568"
     CDS             complement(17408..18427)
                     /gene="gpsA"
                     /locus_tag="OVW45_002568"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCV5526987.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(18427..18894)
                     /gene="secB"
                     /locus_tag="OVW45_002569"
     CDS             complement(18427..18894)
                     /gene="secB"
                     /locus_tag="OVW45_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCV5526988.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSVVSRGTFPQLNLAPINFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(18957..19208)
                     /gene="grxC"
                     /locus_tag="OVW45_002570"
     CDS             complement(18957..19208)
                     /gene="grxC"
                     /locus_tag="OVW45_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCV5526989.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(19350..19781)
                     /locus_tag="OVW45_002571"
     CDS             complement(19350..19781)
                     /locus_tag="OVW45_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCV5526990.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            20026..21570
                     /gene="gpmM"
                     /locus_tag="OVW45_002572"
                     /gene_synonym="pgmI"
     CDS             20026..21570
                     /gene="gpmM"
                     /locus_tag="OVW45_002572"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCV5526991.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            21580..22863
                     /gene="envC"
                     /locus_tag="OVW45_002573"
     CDS             21580..22863
                     /gene="envC"
                     /locus_tag="OVW45_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCV5526992.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIATKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            22867..23826
                     /locus_tag="OVW45_002574"
     CDS             22867..23826
                     /locus_tag="OVW45_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCV5526993.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPNNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASVN"
     gene            complement(23813..>23954)
                     /locus_tag="OVW45_002575"
     CDS             complement(23813..>23954)
                     /locus_tag="OVW45_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCV5526994.1"
                     /translation="MYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHHRITRSAFN
                     LR"
BASE COUNT         6045 a         6109 c         6211 g         5589 t
ORIGIN      
        1 cagcaccacg taagttaaaa caatcaatag atccataatt agatctccgg gaacgtggaa
       61 tagtcagtct ttttctcggc ggcgagttga tgctctagcc gggaaattcg gtcagaaagt
      121 gcggcgattt ccgggtcagt cgctttctcc tgcgacgcaa catgtgactg catgccccac
      181 ccgcgctccg gttgatagag cgtcgcccag atccacagga acggccagat aacatgcaga
      241 gtaaacaggc tcacccagcc cgccgtatga atagcgtcgg catggggatg gttgcgcttt
      301 ttggcaatca gataagggat gtcatgtatc gcgatgattc cataaaaaat caccagaaag
      361 acgaagatca gcactcccaa cgcgaaatag tttagaaaca tatctgcctc ggattcacgt
      421 ttatcagtgt tgtttttggg ctggcagcca gaagggagtc aggctgatat tttgacaata
      481 atccgggttc gcgattctcg ccataacacc aaagaataat ttttagaggt gatgagttgc
      541 ttagttacat aacaattgta tgacgaaggc ataacatgct gtagatcaca tcaggtgaac
      601 gccgtaagaa aatatcttgt gattcagatc acaaagattc aacaaaccat caaaacaaaa
      661 atgtgacact actcacattt aaatgccatt tttagcgaaa atcgccgcct tgttgctttt
      721 ttacacaagc gttttgtgat gaacgtcacg ttaattacct ctctaccccc tatatttatg
      781 tgattgatat cacacaaaag gccgtcgact ggacagttaa ccgattcagt gccagatttc
      841 gcagtatcta caaggtccgg ctacctctgc cgccacatta acaaaaaacc tcgggcttcc
      901 agcctgcgcg acagcaaaca taagaagggg tgtttttatg tcatccgata ttaagatcaa
      961 agtgcaaagc tttggtcgtt tcctcagcaa catggtgatg ccaaatatcg gcgcgtttat
     1021 cgcgtggggt atcatcaccg cgttatttat tccaacaggg tggttaccga acgagacgct
     1081 ggcgaagctg gtcgggccga tgatcactta tctcctgccg ctgctgatcg gttataccgg
     1141 tggtaagctg gtaggcggcg aacgtggcgg cgtagtcggt gccatcacca ccatgggcgt
     1201 tatcgtcggc gcagacatgc cgatgttcct cggttctatg attgcaggtc cgctgggcgg
     1261 ctggtgcatt aagcacttcg accgctgggt agacggtaag atcaaatccg gttttgagat
     1321 gctggtgaat aacttctccg caggcatcat cgggatgatc ctcgctattc tggcattcct
     1381 cggcattggc ccgattgttg aagccctgtc caaaatgctg gctgcgggcg ttaacttcat
     1441 ggttgtccat gacatgctgc cgctggcgtc tatctttgtt gaaccggcga aaatcctgtt
     1501 cctcaacaac gccattaacc acggtatctt ctcgccgctg ggtattcagc agtcccatga
     1561 actgggtaaa tcaatcttct tcctgattga agctaaccca ggtccaggta tgggcgtgct
     1621 gctggcgtac atgttctttg gtcgtggtag cgctaaacag tctgcgggcg gtgcggcaat
     1681 catccacttc ctgggtggta tccacgaaat ctacttcccg tatgtgctga tgaatccgcg
     1741 tctgatcctc gcagtcatcc tcggcggtat gactggcgtg ttcacgctga ctatcctggg
     1801 cggtggtctg gtttctccgg catctccggg ttctatcctt gctgtactgg cgatgacacc
     1861 aaaaggtgct tatttcgcta acatcgcggg tgtgtgtgcg gcgatggctg tctccttcgt
     1921 tgtctctgct attttgctga aaaccagcaa agtgaaagaa gaagatgata ttgaagcagt
     1981 aactcgtcgt atgcaggaca tgaaagctga gtctaaaggc acatctccgc tgtctgcggg
     2041 cgatgtgact aacgacctga gccacgtacg taaaatcatc gttgcctgtg acgccggtat
     2101 gggttccagt gcgatgggcg caggcgtgct gcgtaagaaa attcaggatg caggtctgtc
     2161 acagatttcg gtcactaaca gcgcgatcaa caacctgccg ccagatgtgg acctcgtcat
     2221 cactcaccgt gacctgaccg aacgcgctat gcgccaggtt ccgcaggcgc agcatatttc
     2281 gctgaccaac ttcctcgaca gcggcctgta caccagccta accgaacgtc tggttgctgc
     2341 ccagcgccat actgaaaacg aagtgaaagt gaaagacagt ctgaaagaca gctttgacga
     2401 ttccagtgct aacctgttca aactgggcgc ggagaacatc ttcctcggtc gcaaagcagc
     2461 aaccaaagaa gaagcgattc gttttgctgg cgagcagctg gtgaaaggcg gttacgttga
     2521 gccggaatac gttcaggcga tgctggatcg tgaaaaactg accccgactt atctgggtga
     2581 gtctatcgcg gtgccacacg gtacggttga agcgaaagat cgcgtactga aaacgggcgt
     2641 ggtgttctgc cagtacccgg aaggcgtgcg cttcggtgaa gaagaagatg acattgcccg
     2701 tctggtgatt ggtattgcag cccgtaacaa cgagcacatt caggttatca ccagcctgac
     2761 caatgcactg gatgatgagt ccgtcatcga gcgtctggca cacaccacca gcgtggatga
     2821 agtgctggaa ctgttggcag gtcgtaagta atccaatccc accctctcca catggagaag
     2881 gtggggttaa ttgcctgatg cgctacgctt atcaggccta caggatgcat cacgatttgt
     2941 tgaatttgca cgttcttgta ggccggataa ggcgcttacg ccgcatccgg cgctgcccct
     3001 ctcctcacgg agagggtttg ggtgagggaa aagcctcacc ccagccctct cgggtaaaaa
     3061 cattgatgaa ggttaatact atgaaagcat tacattttgg cgcaggtaat atcggtcgtg
     3121 gctttatcgg taaactgctg gcagacgcgg gtatccaact gacgtttgcc gatgtcaatc
     3181 aggtggtact tgatgccctg aatgcccgtc atagctatca ggttcatgtg gttggtgaaa
     3241 ccgagcaggt agataccgtt tccggcgtca atgctgtcag cagcattggt gatgatgtcg
     3301 ttgatctgat tgctcaggtc gatttagtca ctaccgccgt tggcccggtt gtgctggaac
     3361 gtattgctcc ggctatcgcc aaagggctgg tgaaacgcaa agaacaaggt aatgaatccc
     3421 cgctgaacat catcgcctgt gaaaacatgg tacgcggtac cacgcagctg aaaggccatg
     3481 tgatgaacgc cctgccggaa gacgccaaag cgtgggtaga agaacacgtt ggctttgtcg
     3541 attccgccgt tgaccgcatc gtaccgcctt cggcttcggc aactaacgat ccgctggaag
     3601 tgacggtaga aactttcagc gaatggattg tcgataaaac gcagttcaaa ggcgcactgc
     3661 cgaacatccc aggcatggag ttaaccgaca acctgatggc atttgtcgaa cgtaaactct
     3721 tcaccctgaa cacgggtcat gctataaccg cgtacctcgg aaaactggcc ggtcatcaga
     3781 ccattcgtga cgctattctc gacgagaaaa tccgcgcggt ggtaaaaggt gcgatggaag
     3841 aaagtggtgc ggtactgatc aagcgctacg gctttgacgc agacaaacat gcggcgtaca
     3901 tccagaaaat cctcggtcgt tttgagaacc cgtatctgaa agatgatgta gagcgcgtag
     3961 gccgtcagcc gctgcgtaaa ctgagtgctg gcgaccgtct gatcaagcca ttgctcggta
     4021 cgctggaata cagccttccg cacaagaatc tgattcaggg gattgctggt gcaatgcact
     4081 tccgcagtga agatgatccg caggctcagg aactggcagc actgatcgct gacaaaggtc
     4141 cgcaggcggc gctggcacag atttccgatc ttgatgccaa cagcgaggtt gtatccgagg
     4201 cggtaaccgc ttataaagca atgcaataat ggtggaccag gcgcaggaca ccctgcgccc
     4261 gaataacaga ttgtcagata tgcaggcaac aatggaacaa acccaggcct ttgaaaaccg
     4321 tgtgcttgag cgtctgaatg ctggcaaaac cgtgcgaagc tttctgatca ccgccgtcga
     4381 gcttttgacc gaggcggtaa atcttctggt gcttcaggta ttccgcaaag acgattacgc
     4441 ggtgaagtat gctgtagaac cgttactcga cggcgacggt ccgctgggcg atctttctgt
     4501 gcgtttaaaa ctcatttacg ggttgggcgt cattaaccgc caggaatacg aagatgcgga
     4561 gctgctgatg gcattgcgtg aagaactaaa tcacgacggc aacgagtacg cctttaccga
     4621 cgacgagatc cttggaccct ttggtgaact gcactgcgtg gcggcgttac caccgccgcc
     4681 acagtttgaa ccagcagact ccagtttgta tgcaatgcaa attcagcgct atcaacaggc
     4741 tgtgcgatca acaatggtcc tttcactgac tgagctgatt tccagaatca gcttaaaaaa
     4801 agcctttcaa aagtaagcaa cgtctgctta ctgcccctct acctgcttcg gccgataaag
     4861 ccgacgataa tactccagac gttgtagata taacggcacg ctttcatcgg gtattccgga
     4921 cggaatcgcg ttacggggag gaagtttttt cagatactcc cggaacgcct ggcttgatgc
     4981 catgaaatct acggctttat cgataagaag cggaacgttt tcacctaatt tgcccatgat
     5041 gatatctccg tattaccccg cgccgggaat gcgcggccgc caattttagt ttaggttccc
     5101 ggtaaagatg acattaagga aaacgtgctg aatcctcaat aatcatccgc tgctgtttat
     5161 tatctttttt ctatagattt cttaattaat caataaattg cgtagttttt aagcatgtta
     5221 atgattacgt tacagaacta ttgcaatgtc atcagttgtg aacaaagcac ctggtcgcgc
     5281 atactagggg ctataaattt atctttatca gaagccatca catgaaagaa gtcgaaaaaa
     5341 acgaaatcaa acgtctcagc gatcgtctgg acgccatccg ccaccagcag gccgatctgt
     5401 cgctggttga agccgcagac aaatatgccg agctggaaaa agagaaagcc acgctggaag
     5461 cagaaattgc tcgcctgcgt gaagttcata gccaaaaact gagtaaagaa gcacagaagc
     5521 tgatgaagat gccgtttcag cgcgcaatta ccaaaaaaga gcaggctgat atgggcaagc
     5581 tgaagaaaag tgttcgcgga ctggtggttg tgcacccaat gaccgcgctg ggccgtgaaa
     5641 tgggcctgca ggagatgact ggattctcta agactgcgtt ttaagcgcct cagacgactg
     5701 gttcgatcat atcaacccgc atctggcttg ccttagctgg tcaatgcggg gactctcctg
     5761 tctactcgcc taattaccaa acaattcact ccctttacgt cgatttaccg ctggttgata
     5821 ttctgaaaat gcatttttac cgaaatagcc acctataaac aataaaaata tatagaagat
     5881 atcatttatt aattaacaag cagttacgtc tctttttttc ggatttaatt gtgaaatttt
     5941 aatcatcata ttcatacaat aaattatctg taaatttttt cccagacatg gtttctttca
     6001 tatttgacat ttattaataa tcatcattag aatccacgaa aataatatat tcatacaaac
     6061 acagacatct tttgaaacga atgatacatt tttatgatga atgcttaaaa caatatttaa
     6121 tcgacacaca tcactgtcat taatgtaaag caacatagat caccaataat acaatggaca
     6181 actctaccgg cattgatcgc tggtcggagg aaataaggat ttttacatga acttgaagaa
     6241 gatttttttc agtgctgtca ctgtttcagt tctctgcgca ttaacgggat gtgactacat
     6301 tgaagaaggc aagccagaaa gtagtttatt gaaacaacaa gaagaacatt aaaattgatc
     6361 tacttgaaaa acaacaagca cagctaaaga gtcaactgga aactattcaa aaacaacaaa
     6421 ctggcataat aagcagtaca aagactttaa ctcacgtaat taaatccgtt aaagatcaac
     6481 aaaacacttt tatttttacc gagtttaacc cagcaaaaac gaagtatttc atcctgaaca
     6541 atggttcggt ggctttagcg ggtcgagtgt tatccattga cgccacagag aatggcagtg
     6601 ttattcatat ttcactggtc aacttattaa gtacacctat ctcaaatatt ggttttaatg
     6661 cgacatgggg tggtgaaaaa cctgtcgatg ccaaagagtt tgcccggtgg caacaattac
     6721 ttttcaacac atcaatgaaa tccacattga aattattacc aggtcaatgg caagacatta
     6781 atttgaccct gaagggtgta tcgcccaata acctgggata tctgaagtta gccatcaaca
     6841 tggaaaatat tcagttcgac aatcttccct ctgctgaaaa tcggcagaaa agaagcaaaa
     6901 aataaatgca gttagtacgc ttataacaag gaatagcgag catcattaat gaacaaaata
     6961 tttaaagtta tctggaaccc tgcgacaggg aattatactg ttaccagcga aacggcaaaa
     7021 agccgtggca agaaatctgg gcgcagtaag ctgttaattt ctgcgctggt tgcgggtgga
     7081 atgttgtcgt cgtttggggc attggcgaat gccgggaatg acaacggcca gggtgttgat
     7141 tacggtagtg gatcagctgg cgacagctgg gttgctatag gcaaaggggc gaaagcaaat
     7201 acttttatga acaccagtgg ttccagtact gctgtgggtt atgacgctat agctgaaggc
     7261 caatatagct ctgccatcgg gtcaaaaacc catgcgattg gtggtgcatc aatggccttt
     7321 ggggttagtg caatatcaga aggcgataga agtatagcac tgggtgcctc ttcgtattca
     7381 ttgggccaat actcaatggc cctcggccgt tattcaaaag cattgggtaa attgtctatt
     7441 gctatggggg actcttccaa agcggaagga gcaaacgcca ttgccctggg aaatgccact
     7501 aaagctactg agattatgag tattgctctt ggcgacaccg ccaatgcgtc aaaagcgtat
     7561 tcaatggcgc tgggagcaag tagcgtcgca tctgaagaaa acgcaattgc cctggggcgt
     7621 agcagtgtag ctagcggtac tgacagcctc gcatttggca gacaatcact tgccagcgca
     7681 gcgaacgcta ttgcgatagg tgctgagacc gaagccgctg aaaatgcaac tgctattggc
     7741 aataatgcga aggcaaaagg gactaatagc atggcaatgg ggttcggaag ccttgccgat
     7801 aaagtcaata ctatcgcatt aggaaatggc agccaggctc tggcagataa tgcaatcgcc
     7861 ataggccagg gcaacaaagc tgatggcgtg gatgccatcg ctctgggtaa tggtagccag
     7921 tcgagaggct taaacaccat tgccttaggc acagccagta atgcaactgg tgataagagt
     7981 cttgcgcttg gtagtaatag cagtgccaac ggtattaact ctgtcgcgct gggcgcagat
     8041 tccattgcgg atttagacaa taccgtctct gtcggcaata gttcattaaa acgcaagatc
     8101 gttaatgtga aaaatggcgc gatcaagtct gacagttacg atgccattaa tggttcacag
     8161 ctttatgcca ttagcgactc ggtagcaaaa aggcttggag gaggggctgc agtagatgtt
     8221 gatgacggta ctgttacagc accaacctac aatttaaaaa atggtagcaa aaataacgta
     8281 ggggctgcgc tcgctgtact tgatgaaaac accctgcaat gggaccaaac caaaggcaaa
     8341 tacagcgctg ctcatggtac tagtagccca actgccagcg taatcaccga tgttgcggat
     8401 ggcacgattt cagcctccag taaggatgcg gttaacggtt cccaactgaa agctaccaat
     8461 gacgatgtcg aagccaacac cgccaatatc gctactaata ccagcaacat tgccacgaat
     8521 acagcaagta ttgccaccaa taccaccaat atcaccaacc tgacggattc cgttggtgac
     8581 cttcaggctg atgccctgct ctggaacgaa actaaaaagg cattcagtgc agctcacggc
     8641 caggatacca ccagcaaaat caccaacgtt aaagatgccg acctgacggc tgacagcact
     8701 gatgctgtta acggctctca gctgaaaacc accaacgatg ctgtggcgac gaataccacc
     8761 aatatcgcca ataacacttc caatattgcc actaacacca ccaacatctc taatctgact
     8821 gagacggtga ctaatcttgg tgaggatgcg ctgaaatggg ataaggacaa tggtgtattc
     8881 acggcagctc atggcaccga gaccaccagc aaaatcacca acgttaaaga tggcgacctg
     8941 acgactggca gcaccgatgc cgttaacggc tctcagctga aaaccaccaa cgatgccgtg
     9001 gcgacgaata ccaccaatat cgccactaac accaccaaca tctctaatct gactgagacg
     9061 gtgactaatc ttggtgagga tgcgctgaaa tgggataagg acaatggtgt cttcactgca
     9121 gctcatggca acaataccgc cagcaaaatc accaatatcc tggacggcac agtcactgca
     9181 accagttccg atgccattaa cggtagccag ctttatgact taagcagcaa tatcgccacc
     9241 tacttcggcg gcaatgcttc tgtgaatact gacggtgtgt ttaccggtcc aacctacaaa
     9301 atcggtgaaa caaattatta taacgtcggc gatgcactgg ctgcgattaa ctcctcattt
     9361 agcacgtctc tcggcgatgc tctgctttgg gatgccaccg caggtaaatt cagtgccaaa
     9421 cacggtacta atggtgacgc aagcgtgatc actgatgtcg cagatggtga aatttcagac
     9481 tccagttctg acgcagtaaa cggctcacaa ctccacggcg tgagcagtta tgttgttgat
     9541 gcgctggggg gtggtgccga agtcaatgca gacggcacca tcactgcgcc gacgtacacc
     9601 attgctaatg ctgattacga taatgtcggt gatgccctga atgctatcga taccactctt
     9661 gacgacgctc tgctctggga tgcggacgcc ggtgaaaatg gtgcatttag cgccgctcac
     9721 ggaaaagata aaactgccag tgtaatcact aacgtcgcta acggtgcaat ctctgctgcc
     9781 agcagcgacg cgattaacgg ctcacaactc tataccacca ataagtacat cgctgatgcg
     9841 ctgggtggtg acgcagaagt caacgctgac ggcaccatca ccgcaccgac ttacaccatt
     9901 gcgaacgccg agtacaacaa cgtcggtgac gccctggatg cgcttgatga taacgccctg
     9961 ctgtgggatg agactgccaa tggcggtgct ggagcctaca atgccagcca tgacggtaaa
    10021 gccagcatca tcactaatgt cgctaatggc agtattagtg aggacagtac cgatgcagtg
    10081 aacggttctc agttgaatgc gacgaatatg atgattgagc agaacaccca aattatcaat
    10141 cagctcgctg gtaacaccga cgcaacctat atcgaagaaa atggcgctgg tatcaactac
    10201 gttcgtacta acgacaacga tttagccttt aacgatgcaa gcgcctctgg tgtcggcgct
    10261 acagctgtag gttataacgc tgtcgcgtct ggtgccagca gcgtagccat tggtcagaac
    10321 agcagcagca ccgttgatac cggtattgcg ctgggtagca gctccgtttc cagccgtgtg
    10381 attgccaaag gttctcgtga cactagcgta acggaaaatg gcgtggtgat tggttatgac
    10441 accactgacg gcgaactgct aggtgcattg tcaattggtg atgacggtaa ataccgccaa
    10501 atcatcaacg tagctgatgg ttcagaagcc catgacgccg ttacggttcg ccagttgcag
    10561 aacgctattg gagcggtcgc cactacgcca accaagtact ttcacgccaa ctcaacggca
    10621 gaagactcac tggccgttgg tgaagactcg ctggcaatgg gtgcgaaaac tgtcgttaat
    10681 ggtaatgcag gtattggtat cggtttgaac actctggttc tagctgatgc gatcaacggc
    10741 attgctatcg gcagtaacgc acgcgcaaac catgcaaaca gtatcgcaat gggtaacggt
    10801 tctcagacca cccgtggtgc acagactggc tacaccgcct acaacatgga cgcaccgcag
    10861 aactctgtag gtgagttttc tgtcggtagt gaagacggtc aacgtcagat cacaaacgtc
    10921 gcagctggtt cagcggatac cgatgcggtt aacgtgggtc agttgaaagt cactgatgag
    10981 cgcgtagcgc aaaataccca gagcattact aacctgaaca atcaggtcac taatctggat
    11041 actcgcgtta ctaatatcga aaacggtatt ggcgacattg tcaccaccgg tagcaccaag
    11101 tacttcaaga ccaacaccga tggcgtagat gccaacgccc agggtaaaga tagcgttgct
    11161 attggttctg gttccattgc tgccgctgac aacagcgtcg cactgggtac cggttccgtt
    11221 gcagaggaag aaaatacaat ctctgtaggt tcttctacta accaacgccg tatcaccaac
    11281 gtagctgccg gtgttaatgc caccgatgcg gttaacgtgt cgcagctgaa atcttctgaa
    11341 gcaggcggcg tgcgctacga caccaaagct gatggttcta tcgactatag caatatcacc
    11401 ctcggtggtg gcaacggcag tacgactcgt atcagcaacg tctccgctgg cgtcaacaac
    11461 aacgacgcgg tgaactacgc gcagttgaag caaagcgtgc aggaaacgaa gcaatacacc
    11521 gatcagcgga tggttgagat ggataacaaa ctgtctaaaa ctgaaagcaa gttgagcggt
    11581 ggtatcgctt ctgcaatggc aatgaccggt ctgccgcagg cttatacacc gggtgccagc
    11641 atggcttcta ttggtggcgg tacttacaac ggtgaatcgg cagttgcttt aggtgtatcg
    11701 atggtgagcg ccaatggtcg ttgggtctac aaattacaag gtagtaccaa tagccagggt
    11761 gaatactccg ccgcactcgg tgccggtatt cagtggtaat gatccattaa gttagtgtga
    11821 ctaagggcaa acgccaaatt gcctgatgcg ttacgcgcat caggcaagat aaggcgcccc
    11881 gggtcaacaa tctaagggca attctctgat gaggattgcc cttttcttta ccagacatct
    11941 ccccccacaa gaattggccc taccaattct tcgcttatct gacctctggt tcacaattcc
    12001 ccaattaaaa ctcacatcaa tattgccaat acataacatt tagttaacca ttcattgtca
    12061 ttatccctac acaacacaat tggcagtgcc acttttacac aacgtgtgac aaggagatga
    12121 gcaacagact cattacacga tgtgcgtgga ctccaggaga cctgcaatga atctctggca
    12181 acaaaactac gatcccgccg ggaatatctg gctttccagt ctgatagcat cgcttcccat
    12241 cctgtttttc ttctttgcgc tgattaagct caaactgaaa ggatacgtcg ccgcctcatg
    12301 gacggtagca atcgcccttg ccgtggcttt gctgttctat aaaatgccgg tcgctaacgc
    12361 gctggcctcg gtggtttatg gcttcttcta tggtttgtgg cccatcgcgt ggatcattat
    12421 tgcagcagtg ttcgtctata agatctcggt gaaaaccggg cagtttgaca tcattcgctc
    12481 gtctattctt tcgataaccc ctgaccagcg tctgcaaatg ctgatcgtcg gtttctgttt
    12541 cggcgctttc cttgaaggag ccgcaggctt tggcgcaccg gtagcaatta ccgccgcatt
    12601 gctggtcggc ctgggtttta aaccgctgta cgccgctgga ctgtgcctga ttgttaacac
    12661 cgcgccagtg gcatttggcg cgatgggcat tccaatcctg gttgccggac aagtaacagg
    12721 catcgacagc tttgagattg gccagatggt ggggcggcag ctaccgttta tgaccattat
    12781 cgtgctgttc tggatcatgg cgattatgga cggctggcgc ggtatcaagg agacgtggcc
    12841 tgcggtcgtg gttgcgggcg gctcgtttgc catcgctcag taccttagct ctaacttcat
    12901 tgggccggag ctgccggaca ttatctcttc gctggtatca ctgctctgcc tgacgctgtt
    12961 cctcaaacgc tggcagccag tgcgcgtatt ccgttttggt gatttggggg cgtcacagat
    13021 tgatatgacg ctggcccaca ccggttacac tgcgggtcag gtgttacgtg cctggacacc
    13081 gttcctgttc ctgacagcta ccgtaacact gtggagcatc ccgccgttta aagccctgtt
    13141 cgcatcgggt ggtgcgctgt atgagtgggt gatcaacatt ccggtgccgt acctcgataa
    13201 actggttgcc cgtatgccac cagtggtcag cgaggctaca gcctatgccg ccgtgtttaa
    13261 gtttgactgg ttctctgcca ccggcaccgc cattctgttt gctgcactgc tctcgattgt
    13321 ctggctgaag atgaaaccgt ctgacgctat cagcaccttc ggcagcacgc tgaaagaact
    13381 ggctctaccc atctactcca tcggcatggt gctggcgttc gcctttatct cgaactattc
    13441 cggactgtca tcaacactgg cactggcact ggcgcacacc ggtcatgcat tcaccttctt
    13501 ctcgccgttc ctcggctggc tgggggtatt cctgaccggg tcggatacct catctaacgc
    13561 cctgttcgcc gcgctgcaag ccaccgcagc acaacaaatt ggcgtctctg atctgttgct
    13621 ggttgccgcc aataccaccg gtggcgtcac cggtaagatg atctccccgc aatctatcgc
    13681 tatcgcctgt gcggcggtag gcctggtggg caaagagtct gatttgttcc gctttactgt
    13741 caaacacagc ctgatcttca cctgtatggt gggcgtgatc accacgcttc aggcttatgt
    13801 cttaacgtgg atgattcctt aatgattgtt ttacccagac gcctgtcaga cgaggttgcc
    13861 gatcgtgtgc gggcgctgat tgatgaaaaa aacctggaag cgggcatgaa gttgcccgct
    13921 gagcgccaac tggcgatgca actcggcgta tcacgtaatt cactgcgcga ggcgctggca
    13981 aaactggtga gcgaaggcgt gctgctcagt cgacgcggcg gcgggacgtt tattcgctgg
    14041 cgtcatgaca catggtcgga gcaaaacatc gtccagccgc taaaaacgct gatggccgat
    14101 gatccggatt acagtttcga tattctggaa gcccgctacg ccattgaagc cagcaccgca
    14161 tggcatgcgg caatgcgcgc cacacctggc gacaaagaaa agattcagct ttgctttgaa
    14221 gcaacgctaa gtgaagaccc ggatatcgcc tcacaagcgg acgttcgttt tcatctggcg
    14281 attgccgaag cctcacataa catcgtgctg ctgcaaacca tgcgcggttt cttcgatgtc
    14341 ctgcaatcct cagtgaagca tagccgtcag cggatgtatc tggtgccacc ggttttttca
    14401 caactgaccg aacaacatca ggctgtcatt gacgccattt ttgctggtga tgccgacggg
    14461 gcgcgtaaag caatgatggc gcaccttagt tttgttcaca ccaccatgaa acgattcgat
    14521 gaagatcagg ctcgccacgc acggattacc cgcctgcccg gtgagcataa tgagcattcg
    14581 agggagaaaa acgcatgatt atttccgcag ccagcgatta tcgcgccgca gcgcaacgca
    14641 ttctgccgcc gttcctgttc cactatatgg atggtggtgc atattctgaa tacacgctgc
    14701 gccgcaacgt ggaagatttg tcagaagtgg cactgcgcca gcgtattctg aaaaacatgt
    14761 ccgacttaag cctggaaacg acgctgttta atgagaaatt gtcgatgccg gtagcactgg
    14821 caccggtggg tttgtgtggc atgtatgcgc gtcgtggcga agttcaggca gccaaagcgg
    14881 cggacgcgca tggcattccg tttactctct cgacggtttc cgtttgcccg attgaagaag
    14941 tcgcgccagc catcaagcgc ccaatgtggt tccagcttta tgtactgcgc gatcgcggct
    15001 ttatgcgtaa cgcgctggag cgagcaaaag cagcgggttg ttcgacgctg gttttcaccg
    15061 tggatatgcc gacaccgggc gcacgctacc gtgatgcgca ttcaggtatg agcggcccaa
    15121 atgcggcaat gcgccgctac ttgcaggcgg tgacgcatcc gcaatgggcg tgggatgtgg
    15181 gcctgaacgg tcgtccacat gatttaggta atatctcagc ttatctcggc aaaccgaccg
    15241 gactggaaga ttacatcggc tggctgggga ataacttcga tccgtccatc tcatggaaag
    15301 accttgagtg gatccgcgat ttctgggatg gcccgatggt gatcaaaggg atcctcgatc
    15361 cggaagatgc gcgcgatgca gtacgttttg gtgctgatgg aattgtggtt tctaaccacg
    15421 gtggccgcca gctggacggt gtactctctt ccgctcgtgc gttgcccgct attgcagatg
    15481 cggtgaaagg tgatatcgcc attctggcgg atagcggaat tcgtaacggg cttgatgtcg
    15541 tgcgtatgat tgcgctcggt gccgacaccg tactgctggg tcgtgctttc ctgtatgcgc
    15601 tggcaacagc gggccaggcg ggtgtagcta acctgctaaa tctgatcgaa aaagagatga
    15661 aagtggcgat gacgctgact ggcgcgaaat cgatcagtga aattacgcaa gattcgctgg
    15721 tgcaggggct gggtaaagag ttgcctacgg cactggctcc gatggcgaaa gggaatgcgg
    15781 catagtcgtt tgcgccccct caccctaacc ctctccctca gggagagggg accgttcggc
    15841 gctgtatgta ctccctcact ctgaaacgac accgcactct ttttttctcc ctcgcccctc
    15901 cggggagggc cggggtgagg ggaaaaggcc gcacggttca ccaattttct gctatcctgc
    15961 ccccgcacta agggggcagt atgctaaaca tcgtacttta cgaaccagaa attccgccaa
    16021 atactggcaa catcatccgt ctttgcgcta ataccggctt tcgtctgcat atcatcgaac
    16081 cgatgggatt tgcctgggac gataagcgcc tgcgccgcgc ggggctggac tatcacgagt
    16141 ttaccgccgt tacgcgtcat catgactatc gcgcgttcct cgaagcagaa aatccccagc
    16201 gcctgtttgc cctcaccacg aaaggtacgc ctgctcacag cgccgtaagc tatcaggatg
    16261 gcgattatct gatgttcggc ccggaaacac gcggcctgcc agcgagcatt cttgatgccc
    16321 tgcccgctga acaaaaaatt cgcattccga tggtgccgga cagccgcagc atgaatctgt
    16381 ccaatgcggt gtcggtagtg gtgtatgaag cctggcggca gctgggatat ccgggagcgt
    16441 tgttgagaga ttagttactg tatgccggat agatgattac atcgcatccg gcacgatcac
    16501 aggacattag atcccatccc catactcaaa tgtatggtta ataccgttga aatgctggtc
    16561 catatccatt gatggcttat cgctgtctgg tttaccgaca atacgagccg gaacgccagc
    16621 ggcggttgta tgcggcggca ccggttgcag caccacggaa cctgcgccaa tcttcgcgcc
    16681 gcgaccaact tcaatattgc cgaggatttt cgcgcccgcg ccaatcatca caccttcacg
    16741 aattttcggg tgacggtcac caccagattt acccgtaccg ccaagcgtca cagattgcag
    16801 aatcgatacg tcgttttcaa ttaccgccgt ttcacccaca acgatgcccg tggcgtggtc
    16861 gagcataatg ccacggccaa tttttgctgc cgggtgaata tcgacctgga acgtcacaga
    16921 aacctggttt tgcagaaaga ttgccaatgc gcgacgcccc tgattccaca accagtgacc
    16981 gatgcgatag gcctgcaagg catgaaaacc cttcaggtat aacaacgggg ttgagtattt
    17041 atcgactgcc gggtcgcggg tacgcaccgc ctgaatatca caggccgcag aggcgatcat
    17101 ttccgggtca gcggcgtagg cttcttccac cacttcacgg atagcaatag caggcataat
    17161 tggcgatgac agcttgttcg ccagcatgta gctcagtgca ctaccaaggt tttcgtgctt
    17221 gagtagcgtc gcgtggtaaa aactggccag cattggctca cagtccgcca gcgttctggc
    17281 ttcggcttta atattgttcc agacaatttc cagttcttca cacgacattg cttactccac
    17341 acgatgagat aatgaccggc ccgttctgcg cgggccgggt catagcggta acaaaggttc
    17401 cctggggtta gtggctgctg cgctcgtcct tgcgtgcacg acctaataac gtcaatgctg
    17461 cctcgcgcgc gttttttccg caatataata cttgataaat ttcctcggtt attggcattt
    17521 ctacgccgaa gcgatgcgcc agttcgcgga cttctttcgt attgcggtag ccttccacca
    17581 cctgaccaat cttctcctgc gcgctttgta catccatgcc ctgaccgagc atcatgccaa
    17641 aacggcggtt acgcgactgg ttgtcggtac aggtaagcac cagatcgccc agccccgcca
    17701 tgcccataaa ggtggcagga tcggcaccca gcgccgcgcc aagacgcgac atttcagcca
    17761 gcccacgggt gatcagtgcc gtacgcgcat tcgcaccaaa accgataccg tcggacatcc
    17821 ccgcgccaat ggcaatgacg tttttcaccg caccgccaag ctgcacaccg atgaaatccg
    17881 ggttgctgta aacgcggaaa cttttgccac agtgcagcaa ttgttggaga tcatcggcaa
    17941 aggtctggtc ggtagaggcc aacgaaatag ccgtcggtaa acctgccgcc agttctttcg
    18001 caaacgttgg gccagagata accgccagcg gaatttgatc gcctaaggcc tcacgcgcca
    18061 cgtcctgtaa cagacgtccg gtttccgctt ccagcccttt ggtcgcccac accagacgcg
    18121 catcaggacg catcagcggt ttaatctggc gcagcacttc accaaagaca tggctgggta
    18181 cgacgacgag aatattacgg ctggctgcca gcgcagtggc gagatcgctt tcaagatgga
    18241 gcgtatcggg aaaaggcaca tcggggagaa acgcggcgtt acagcggtcg cgttcaagcg
    18301 ttgcgatatg ttcagggtca tggccccaga ggacaacctc gtggccattt cttgccaggg
    18361 taatggcaag agcggtgccg tacgagccgg caccgatcac agtcattgaa gcattacgtt
    18421 ggttcatcag gcatcctgat gttcttcagt accttcgcca gcctgctgct gcaaatagtt
    18481 catgaacagc gcatcgaagt taatcggcgc aaggttcagt tgcgggaatg taccgcggga
    18541 taccacgctg gtgatgcact cacgagcata cgggaacaga atgttcgggc agtatgcgcc
    18601 caggcaatgt gccatctggg tgccttcgat acccgcgatg gagaaaatac cgccctgctg
    18661 aacttcacac aggaacgcgg tttcttcgcc cagggaggcc gttacggtaa cacgcagtac
    18721 cacttcgtat acgtcatctg ccagttggga agatgccgta tccagatcaa gtttaacttc
    18781 tggttgccaa tctttctgga aaacgtgcgg cgcgttcggc gcttcgaaag agatatcctt
    18841 ggtataaata cgttggatct ggaaagtcat ttcagtgttg ttttgttctg acatgtgtag
    18901 aaaaccctta agtgttgtcc ttaaatactg cgcaatgtaa tgccaacgtt cacacgttat
    18961 ttcagcaggg gatccagtcc accacgtgca tccaatgcat acaagtcatc acagccgcca
    19021 atgtgctgtg cgtcaataaa aatctgggga accgtggtgc gaccgctgcg tttgatcatc
    19081 tcttcacgct tggcggcatt gccatcgatc ggcaactcct gaaaactcac gcccttgctg
    19141 ctcagcagtg cttttgcacg atggcaatac gggcaggttt ctttggtata gatttcaaca
    19201 ttggccataa tttagctcct gtttttttac cctgaaattt catgttgcag ggcggctgca
    19261 aaatggagtg tccccagaag cttataacaa taagtcgctg ggggcctaat tgcagctaac
    19321 ggcctgcatc atgaaagacg acaggtaaat tatttgccgc gcaccagagg caagttttcg
    19381 ccagcccagc cagcaacgcc ctctttcagc acgaatactt gcgcaaaacc agctttcgtc
    19441 agcgcgtttg caggctcctg gcactgcatg ccagaaccgt ctaccacgat aaccggtttg
    19501 tctttgtgct tctcaagctc accaacattg ttggctttga tttcgctcgg caacagattg
    19561 atagaacctg cgatatggcc tttacggaag tcatcacgct gacgtaaatc cacaaccaca
    19621 gcgtcttctt tgttgatcag acgcgtagct tcaccacgag taatcacctt cactttcgag
    19681 gtcaggctct taaacgtagt cacaagaacc gccaccagta acgcgatcca ggcgatactc
    19741 agtatgggat gacggccaac aaattgcata atttcttgca tggggggtaa caactcccga
    19801 cgtagtgatt aaaaaaacca ggaaaggagt atacctgcgc ggtgcggcaa atacagccag
    19861 cgcgttaact ggaatgcaat tttgcggggc acgacgaaaa aaaagcgcaa atctggcatc
    19921 ggcttgccgc gagcggtcgt atattttgat cttcagaggc tattttatcg attcagctgt
    19981 agtaaaatta cgcaaatttt gactcttgag tatgaggttg tcgcaatgtc ggtttctaaa
    20041 aaacctatgg tactggtgat tctggatggc tatggctatc gtgaagaaca gcaggataac
    20101 gccattttta gtgctaaaac cccggtaatg gatgcactgt gggccaatcg tccgcatacc
    20161 ctgatcgacg cttccggtct ggaagtcggt ctgcctgacc gtcagatggg taactccgaa
    20221 gtaggccacg ttaacctggg tgccggccgc atcgtgtatc aggacctgac tcgtctggac
    20281 gttgaaatca aagatcgcgc tttctttgct aatccagtgc tgactggcgc ggtagataaa
    20341 gcgaaaaacg caggtaaagc agtacacatt atgggtctgc tctccgcagg cggcgtacac
    20401 agccacgaag atcacatcat ggcgatggta gaactggcag ctgaacgcgg cgcagaaaaa
    20461 atctacctgc acgcattcct tgacggtcgc gacaccccgc cgcgcagtgc tgaatcctcg
    20521 ctgaaaaaat ttgaagagaa atttgctgcg ctgggtaaag gccgcgtagc gtccatcatt
    20581 ggtcgttact acgcgatgga ccgcgataac cgttgggatc gcgttgaaaa agcctatgac
    20641 ctgctgactc tggcgcaggg tgagtttcag gccgatactg ccgttgctgg tttgcaggca
    20701 gcttatgctc gcgacgaaaa cgacgagttc gtgaaagcga ccgttattcg tgcagaaggt
    20761 cagccagatg cggcaatgga agacggcgat gcgctgattt tcatgaactt ccgtgctgac
    20821 cgcgcgcgtg aaatcactcg tgcattcgtg aacgctgatt tcgatggctt cgcgcgtaag
    20881 aaagtggtta acgtcgattt cgtgatgctg accgaatacg ccgctgacat caaaactgcg
    20941 gttgcttacc cacccgcttc cctggttaac accttcggcg agtggatggc gaaaaacgac
    21001 aaaactcagt tgcgtatttc cgaaaccgaa aaatatgccc acgttacttt cttcttcaac
    21061 ggtggcgtag aagagtcgtt caaaggcgaa gatcgcattc tgatcaactc accgaaagtg
    21121 gctacctacg atctgcaacc ggaaatgagc tccgcagagc tgaccgaaaa actggttgcg
    21181 gccatcaaga gcggcaaata cgacaccatt atctgtaact atccgaacgg cgacatggta
    21241 ggtcacaccg gggtgatgga agcggcggtt aaagcggttg aagcactgga tcactgcgtg
    21301 gaagaagtcg cgaaagcggt tgagtccgtg ggtggacaac tgctgatcac cgctgaccac
    21361 ggtaacgctg aacagatgcg cgatccggca acgggtcagg cacacacggc acacaccaac
    21421 ctgccagttc cgctgattta cgttggtgat aagaacgtga aagcggttgc aggcggcaaa
    21481 ctttctgaca tcgcgccgac catgttgtcg ctgatgggta tggaaatccc gcaagagatg
    21541 actggtaagc cgctgttcat cgtggaataa tccctcccca tgaggggaaa ggcgattaat
    21601 accatgacac gggccgtgaa accgcgcagg tttgcaatca ggcccatcat ctacgccagc
    21661 gttcttagcg ctggcgtatt gttgtgcgcc ttttccgccc acgcggatga gcgtgaccaa
    21721 ctcaaatcta ttcaggccga tatcgccaca aaagagcgcg cggtacgcca aaagcaacaa
    21781 caacgcgcaa gcctgctcgc acaattgaaa aagcaggaag aagcgatctc tgaagccacc
    21841 cgtaagctgc gcgaaacgca aaacacgctc aatcaactca ataaacagat tgatgagatg
    21901 aacgcgtcga ttgccaaact ggagcagcaa aaagccgccc aggagcgcag cctcgccgca
    21961 caactggatg ccgcattccg tcagggcgag cataccggta ttcagctaat tctcagcggt
    22021 gaagaaagcc agcgtggaca gcgtttacag gcttatttcg gctatctcaa ccaggcgcga
    22081 caagaaacca ttgcccagtt gaagcaaacg cgtgaagaag tcgccatgca gcgtgctgaa
    22141 ctggaagaga aacaaagcga gcaacaaacg ctgttatatg aacagcgcgc ccaacaggcg
    22201 aaactgactc aggctctgaa cgagcgtaaa aagacgctgg cggggctgga atcttccatc
    22261 cagcaaggtc agcagcagct aagtgaactg cgtgccaacg aatcccgtct gcgtaacagt
    22321 attgcccgtg cggaagccgc ggcgaaagcg cgtgctgaac gagaagcgcg tgaggctcag
    22381 gcagttcgcg acaggcagaa agaagcgacg cgcaaaggca ccacctacaa accgaccgaa
    22441 agcgaaaaat cgctgatgtc ccgaactggt ggcctgggtg cgccgcgtgg tcaggcattc
    22501 tggccagttc gtggccctac gctgcatcgc tatggcgagc agctacaggg tgaattacgc
    22561 tggaaaggaa tggttatcgg tgcctctgaa ggtactgaag ttaaagcaat tgccgacggt
    22621 cgggtgattc tggctgactg gctgcaaggc tacggtctgg tggtggtggt tgagcacggt
    22681 aaaggcgata tgagtcttta cggctataat caaagcgcgc tggtgagcgt tggttcgcag
    22741 gttcgtgcgg gccagccaat tgcactggtg ggcagcagtg gcggtcaggg cagaccttca
    22801 ctctatttcg aaattcgccg ccagggtcag gccgtcaatc cacagccgtg gttgggaaga
    22861 taagttttgt ttccatttcg tcgtaacgtt cttgcatttg ccgctctgtt ggcgctctcc
    22921 tcccctgtaa ttgctggcaa acttgccatc gtcattgatg attttgggta tcgcccgaac
    22981 aacgaaaacc aggtgctggc gatgccttcc gctatctccg tcgctgtatt acccgattca
    23041 ccgcacgcca gagagatggc gaccaaagcg cataacagcg gtcacgaagt gttgattcat
    23101 ctcccgatgg caccgttgag taaacagccg ctggagaaaa atacgctgcg cccggagatg
    23161 agcagcgacg aaattgagcg cattattcgt agtgcggtca ataacgtgcc ctatgccgtg
    23221 gggatcaata accacatggg cagcaagatg acctctaacc tgtttggtat gcagaaagtg
    23281 atgcaggcgc tggagcgtta taatctttac ttcctcgaca gcgtaaccat tggtaatacc
    23341 caggcgatgc gcgccgcgca aggcactggc gtgaaggtga tcaaacggaa ggtgttcctc
    23401 gacgattcac agaacgaagc ggacatccgt gtgcaattca atcgcgcaat tgacctagcg
    23461 cgtcgcaacg gatcaaccat tgccattggg catcctcacc cttcaacggt acgcgtgttg
    23521 caacagatgg tttataacct gccgccagac attacgctgg tgaaagccag cagcttgctg
    23581 aatgaaccgc aggttgatac gtctacaccg ccgaaaaaca ctgtgcccga cacaccgcgt
    23641 aatccgttcc gtggcgtgaa gctgtgcaaa ccgaagaaac cgatagaacc tgtttatgct
    23701 aatcgcttct ttgaagtatt aagcgaaagc atcagccaga gcacgctgat cgtttacttc
    23761 cagcatcagt ggcaaggctg gggcaaacag cctgaagcgg cgaagcttaa cgctagcgta
    23821 aattaaaggc gctacgcgta atgcgatggt gcgaccgcgt tttgtcgcgc cattgccata
    23881 agcggaaaga ccacagtaac gcctgataac ccacctttac gctgcgcaca ttggtaatca
    23941 ggcgcttata cata
//
LOCUS       DAKISA010000063        23937 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.063, whole genome shotgun sequence.
ACCESSION   DAKISA010000063 DAKISA010000000
VERSION     DAKISA010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23937
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.063"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            83..628
                     /locus_tag="OVW45_002576"
     CDS             83..628
                     /locus_tag="OVW45_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HCV5526995.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIRWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            603..2528
                     /locus_tag="OVW45_002577"
     CDS             603..2528
                     /locus_tag="OVW45_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCV5526996.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDIGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            2525..2731
                     /locus_tag="OVW45_002578"
     CDS             2525..2731
                     /locus_tag="OVW45_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HCV5526997.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            2728..4329
                     /locus_tag="OVW45_002579"
     CDS             2728..4329
                     /locus_tag="OVW45_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCV5526998.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     DARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSKEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            4310..5610
                     /locus_tag="OVW45_002580"
                     /pseudo
     CDS             4310..5610
                     /locus_tag="OVW45_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
     gene            5620..5952
                     /locus_tag="OVW45_002581"
     CDS             5620..5952
                     /locus_tag="OVW45_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCV5526999.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            6008..7033
                     /locus_tag="OVW45_002582"
     CDS             6008..7033
                     /locus_tag="OVW45_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCV5527000.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGVTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            7075..7473
                     /locus_tag="OVW45_002583"
     CDS             7075..7473
                     /locus_tag="OVW45_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCV5527001.1"
                     /translation="MTKDELIARLQELGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVVSAQPDTVIQDTAELVTVVALVTLHTDALHATRDK
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            7485..7838
                     /locus_tag="OVW45_002584"
     CDS             7485..7838
                     /locus_tag="OVW45_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HCV5527002.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            7850..8428
                     /locus_tag="OVW45_002585"
     CDS             7850..8428
                     /locus_tag="OVW45_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCV5527003.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRHKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            8425..8820
                     /locus_tag="OVW45_002586"
     CDS             8425..8820
                     /locus_tag="OVW45_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCV5527004.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEEDFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVIIYEM"
     gene            8828..9568
                     /locus_tag="OVW45_002587"
     CDS             8828..9568
                     /locus_tag="OVW45_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCV5527005.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASASVVKGQSTTLTVAFQPDGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            9584..10006
                     /locus_tag="OVW45_002588"
     CDS             9584..10006
                     /locus_tag="OVW45_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCV5527006.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLDLMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            9988..10422
                     /locus_tag="OVW45_002589"
     CDS             9988..10422
                     /locus_tag="OVW45_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCV5527007.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPEMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            10415..12976
                     /locus_tag="OVW45_002590"
     CDS             10415..12976
                     /locus_tag="OVW45_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCV5527008.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKKRLERDAAQKRVDQQRQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVYAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEG
                     INFNQNNHVVIQNDGTNGLPGPQMMKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            12973..13302
                     /locus_tag="OVW45_002591"
     CDS             12973..13302
                     /locus_tag="OVW45_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCV5527009.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEAMVLESFLEEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            13302..14000
                     /locus_tag="OVW45_002592"
     CDS             13302..14000
                     /locus_tag="OVW45_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCV5527010.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLMVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            14006..14749
                     /locus_tag="OVW45_002593"
     CDS             14006..14749
                     /locus_tag="OVW45_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCV5527011.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLAAASTFV"
     gene            14686..15318
                     /locus_tag="OVW45_002594"
     CDS             14686..15318
                     /locus_tag="OVW45_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCV5527012.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRAMATQLPAFRQKLSD
                     GWYQVRIAGQDVSTSGLTAQLHEALPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGS
                     FFTAGATLAAWGAAIGAGGMTGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQN
                     TYFSSLDNMVAQGNVMPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            15379..18876
                     /locus_tag="OVW45_002595"
     CDS             15379..18876
                     /locus_tag="OVW45_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCV5527013.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQSPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGSTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRQSDKVWAYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGIRTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGQGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     HQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAALSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMVNPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     SAFSLTPDGKLTAKNADISGSVNANAGTLNNVTVNENCTIKGMLEATQVRGDFVKAVS
                     KSFPKQAGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGT
                     RYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGGGSVTLEFKIFQKGNQGAGN
                     ITDCTVIVTKKAASGISIR"
     gene            18947..19463
                     /locus_tag="OVW45_002596"
                     /pseudo
     CDS             18947..19463
                     /locus_tag="OVW45_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
     gene            19528..20932
                     /locus_tag="OVW45_002597"
                     /pseudo
     CDS             19528..20932
                     /locus_tag="OVW45_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420356.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
     gene            20881..21033
                     /locus_tag="OVW45_002598"
                     /pseudo
     CDS             20881..21033
                     /locus_tag="OVW45_002598"
                     /inference="COORDINATES: protein motif:HMM:NF015371.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            21010..21189
                     /locus_tag="OVW45_002599"
                     /pseudo
     CDS             21010..21189
                     /locus_tag="OVW45_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003910815.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            21904..22611
                     /locus_tag="OVW45_002600"
                     /pseudo
     CDS             21904..22611
                     /locus_tag="OVW45_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015953155.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            22611..23192
                     /locus_tag="OVW45_002601"
     CDS             22611..23192
                     /locus_tag="OVW45_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCV5527014.1"
                     /translation="MAFRMSEQARTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEKE
                     TLLLEAWKKYRVLLNRVDTSTAPDIEWPVAPIG"
BASE COUNT         5744 a         5976 c         7289 g         4928 t
ORIGIN      
        1 aagaaaggaa acgacaggtg ctgaaaacgg gctttttggc ctctgtcgtt tcctttctct
       61 gtttttgtcc gtggaatgaa caatggaagt caacaaaaag cagctggctg acattttcgg
      121 tgcgagtatc cgtaccattc agaactggca ggaacaggga atgcccgttc tgcgaggcgg
      181 tggcaagggt aatgaggtgc tttatgactc tgccgccgtt ataagatggt atgccgaaag
      241 ggatgctgaa attgagaacg aaaagctgcg ccgggaagtt gaagaactgc ggcaggccag
      301 cgagacagat ctccagccag ggactattga gtacgaacgc catcgactta cgcgtgcgca
      361 ggccgacgca caggagctga aaaatgccag agactccgct gaagtggtgg aaaccgcatt
      421 ctgtactttc gtgctgtcgc ggatcgcagg tgaaattgcc agtattctcg acgggatccc
      481 cctgtcggtg cagcggcgtt ttccggaact ggaaaaccga catgttgatt tcctgaaacg
      541 ggatatcatc aaagccatga acaaagcagc cgcgctggat gaactgatac cggggttgct
      601 gagtgaatat atcgaacagt caggttaaca ggctgcggca ttttgtccgc gccgggcttc
      661 gctcactgtt caggccggag ccacagaccg ccgttgaatg ggcggatgct aattactatc
      721 tcccgaaaga atccgcatac caggaagggc gctgggaaac actgcccttt cagcgggcca
      781 tcatgaatgc gatgggcagc gactacatcc gtgaggtgaa tgtggtgaag tctgcccgtg
      841 tcggttattc caaaatgctg ctgggtgttt atgcctactt tatagagcat aagcagcgca
      901 acacacttat ctggttgccg acggatggtg atgccgagaa ctttatgaaa acccacgttg
      961 agccgaccat ccgcgatatt ccgtcgctgc tggcgctggc tccgtggtat ggcaaaaagc
     1021 accgggataa cacgctcact atgaagcgtt tttccaatgg tcgtggcttc tggtgcctgg
     1081 gcggtaaagc ggcaaaaaac taccgtgaaa agtcggtgga tgtggcgggt tatgatgaac
     1141 ttgctgcctt tgatgaggat attgaacagg aaggctctcc gacgttcctt ggcgacaaac
     1201 gtattgaagg ctcggtctgg ccaaagtcca tccgtggctc cacgcccaaa gtgagaggca
     1261 cctgccagat tgagcgtgca gccagtgaat ccccgcattt tatgcgtttt catgttgcct
     1321 gcccgcactg cggggaggag cagtatctta aatttggcga caaagagacg ccgtttggcc
     1381 tcaaatggac gccggatgac ccctccagcg tgttttatct ctgcgagcat aatgcctgcg
     1441 tcatccgcca gcaggagctg gactttactg atgcccgtta tatctgcgaa aagaccggga
     1501 tctggacccg tgatggcatt ctctggtttt cgtcatccgg tgaagagatt gagccacctg
     1561 acagtgtgac ctttcacatc tggacagcgt acagcccgtt caccacctgg gtgcagattg
     1621 tcaaagactg gatgaaaacg aaaggggata cgggaaaacg taaaaccttc gtaaacacca
     1681 cgctcggtga gacgtgggag gcgaaaattg gcgaacgtcc ggatgctgaa gtgatggcag
     1741 agcggaaaga gcattattca gcgcccgttc ctgaccgtgt ggcttacctg accgccggta
     1801 tcgactccca gctggatcgc tacgaaatgc gcgtatgggg atgggggccg ggtgaggaaa
     1861 gctggctgat tgatcggcag attattatgg gccgccacga cgatgaacag acgctgctgc
     1921 gtgtggatga ggccatcaat aaaacctata cccgccggaa tggtgcagaa atgtcgatat
     1981 cccgtatctg ctgggatatt ggcgggattg acccgaccat tgtgtatgaa cgctcgaaaa
     2041 agcatgggct gttccgggtg atccccatta aaggggcatc cgtctacggt aagccggtgg
     2101 ccagcatgcc tcgtaagcga aacaaaaacg gggtttacct taccgaaatc ggtacggata
     2161 ccgcgaaaga gcagatttat aaccgcttca cactgacgcc ggaaggggat gaaccgcttc
     2221 ccggtgccgt tcacttcccg aataacccgg atatttttga tctgaccgaa gcgcagcagc
     2281 tgactgctga agagcaggtc gaaaaatggg tggatggcag gaaaaaaata ctgtgggaca
     2341 gcaaaaagcg acgcaatgag gcgctcgact gcttcgttta tgcgctggcg gcgctgcgca
     2401 tcagtatttc ccgctggcag ctggatctca gtgcactgct ggcgagcctg caggaagagg
     2461 atggtgcagc aaccaacaag aaaacactgg cagattacgc ccgtgcctta tccggagagg
     2521 atgaatgacg cgacaggaag aacttgccgc tgcccgtgcg gcactgcatg acctgatgac
     2581 aggtaaacgg gtggcaacgg tacagaaaga cggacggcga gtggagttta cggccacttc
     2641 cgtgtctgac ctgaaaaaat acattgcgga gctggaggtg cagaccggca tgacacagcg
     2701 acgcagggga cctgcaggat tttatgtatg aaaacgccca ccattcccac ccttctggga
     2761 ccggacggca tgacatcgct gcgtgaatat gccggttatc acggcggtgg cagcggattt
     2821 ggtgggcagt tgcgggcgtg gaacccaccg ggtgaaagtg tggatgcagc cctgctgccc
     2881 aactttaccc gtggcaatgc ccgcgcagac gatctggtac gcaataacgg ctatgctgcc
     2941 aacgccatcc agctgcatca ggatcatatc gtcgggtctt ttttccggct cagtcatcgc
     3001 ccaagctggc gctatctggg catcggggag gaagacgccc gtgccttttc ccgcgaggtt
     3061 gaagcggcat ggaaagagtt tgccgaggat gactgctgct gcattgacgt tgagcgaaaa
     3121 cgcacgttta ccatgatgat tcgggaaggt gtggccatgc acgcctttaa cggtgaactg
     3181 ttcgttcagg ccacctggga taccagttcg tcgcggcttt tccggacaca gttccggatg
     3241 gtcagcccga agcgcatcag caacccgaac aataccggcg acagccggaa ctgccgtgcc
     3301 ggtgtgcaga ttaatgacag cggtgcggcg ctgggatatt acgtcagcga ggacggctat
     3361 cctggctgga tgccgcagaa atggacatgg ataccccgtg agttacccgg cgggcgcgcc
     3421 tcgttcattc acgtttttga acccgtggag gacgggcaga cccgcggtgc aaatgtgttt
     3481 tacagcgtaa tggagcagat gaagatgctc gacacgctgc agaacacgca gctgcagagc
     3541 gccattgtga aggcgatgta tgccgccacc attgaaagtg agctggatac gcagtcagcg
     3601 atggatttta ttctgggcgc gaacagtaag gagcagcggg aaaggctgac gggctggatt
     3661 ggtgaaattg ccgcgtatta cgccgcagca ccggtccgtc tgggaggcgc aaaagtgccg
     3721 cacctgatgc cgggggactc actgaacctg cagacggctc aggacacgga taacggctac
     3781 tccgtgtttg agcagtcact gttgcggtat atcgctgccg ggctgggtgt ctcgtatgag
     3841 caactttccc ggaattacgc ccagatgagc tactccacgg cacgggccag tgcgaacgag
     3901 tcgtgggcgt actttatggg gcggcgaaaa ttcgtcgcat cccgtcaggc gagccagatg
     3961 tttctgtgct ggctggaaga ggccatcgtt cgccgcgtgg tgacgttacc ttcaaaagcg
     4021 cgcttcagct ttcaggaagc ccgcagtgcc tgggggaact gcgactggat aggctccggt
     4081 cgtatggcca tcgatggtct gaaagaagtt caggaagcgg tgatgctgat agaagccgga
     4141 ctgagcacct acgagaaaga gtgcgcgaaa cgcggtgacg actatcagga aatttttgcc
     4201 cagcaggtcc gtgaaacgat ggagcgccgc gcagctggtc ttaaaccgcc cgcctgggcg
     4261 gctgcggcat ttgaatccgg gctgcgacaa tcaacagagg aggagaagag tgacagcaga
     4321 gctgcgtaat ctcccgcata ttgccagcat ggcttttaat gagccgctga tgcttgaacc
     4381 cgcctatgcg cgggttttct tttgtgcgct tgcaggccag cttgggatca gctgcctgac
     4441 ggatgcagta tccggcgaca gcctgactgc cggagaggca cccgcggcgc tggcgttatc
     4501 cggtgatgat gacggaccac gacaggcccg cagttatcag gtaatgaacg gcatcgccgt
     4561 gctgccggtg tccggcacgc tggtcagccg tactcgggga tgaccggtta caacggcatt
     4621 atcgcccgtc tgcaacaggc tgccagcgac ccgatggtgg acggcattct gctcgatatg
     4681 gacacaccgg gcggaatggt ggcgggagca tttgactgcg ctgacatcat cgcccgtgtg
     4741 cgtgacataa agccggtatg ggcgctggcc aatgacatga actgcagcgc aggtcagctg
     4801 cttgccagtg ccgcctcccg gcgtctggtc acgcagaccg cccggacagg ctccatcggc
     4861 gtcatgatgg ctcacagtaa ttacggcgct gcactggaga aacagggcgt ggaaatcacg
     4921 ctgatttaca gcggcagcca taaggtggat ggcaacccct acagccatct accggatgat
     4981 gtccgggaaa cattgcagtc ccggatggat gcaacccgcc ggatgtttgc acagaaggtg
     5041 tcggcatata ccggcctgtc cgtgcaggct gtgctggata ccgaggctgc agtgtacagc
     5101 ggtcaggagg ccattgatgc cggactggct gatgaacttg ttaacagtac cgatgcgatc
     5161 accgtcatgc gtgatgcact ggatgcacgt aaatcccgtc tctcaggagg gcgaatgacc
     5221 aaagagactc aatcaacaac tgtttcagcc actgcttcgc aggctgacgt tactggcgtg
     5281 gtgcaagcga cggagggcga gaacgccagc gcggcgcagc cggacgtgaa cgcgcagatc
     5341 accgcagcgg ttgcggcaga aaacagtcgc attatgggga tcctcaactg tgaggaggct
     5401 cacggacgcg aagaacaggc acgcgtgctg gccgaaaccc ccggtatgac cgtggaaacg
     5461 gcccgccgta ttctggccgc agcaccacag agtgcacaga cgcgcagtga cactgcgctg
     5521 gatcgtctga tgcagggggc accggcaccg ctggctgcag gtaacccggc atctgatgcc
     5581 gttaacgatt tgctgaacac accagtgtaa gggatgttta tgacgagcaa agaaaccttt
     5641 acccattacc agccgctggg caatagtgac ccggctcata ccgcaaccgc gcccggcgga
     5701 ttgagtgcga aagcgcctgc aatgaccccg ctgatgctgg acacctccac ccgtaagctg
     5761 gttgcgtggg atggcaccac cgacggtgct gccgttggca ttcttgcggt tgctgctgac
     5821 cagaccagca ccacactgac gttctacaag tccggcacgt tccgttatga ggatgtgctc
     5881 tggccggagg ctgccagcga cgagacgaaa aaacggaccg cgtttgccgg aacggcaatc
     5941 agcatcgttt aactttaccc ttcatcacta aaggccgcct gtgcggcttt ttttacggga
     6001 tttttttatg tcgatgtaca caaccgccca gctgctggcg gcaaatgagc agaaatttaa
     6061 gtttgatccg ctgtttctgc gtctcttttt ccgtgagagc tatcccttca ctacggagaa
     6121 agtctatctc tcacaaattc cgggactggt aaacatggcg ctgtacgttt cgccgattgt
     6181 ttccggtgag gttatccgtt cccgtggcgg ctccacctct gaatttacgc cgggatatgt
     6241 caaacccaag catgaggtga atccgcagat gaccctgcgt cgcctgccgg atgaagatcc
     6301 gcagaatctg gcggacccgg cttaccgccg ccgtcgcatc atcatgcaga acatgcgtga
     6361 cgaagagctg gccattgctc aggtcgaaga gatgcaggca gtttctgccg tgctcaaggg
     6421 caaatacacc atgaccggtg aagccttcga tccggttgag gtggatatgg gccgcagtgc
     6481 ggcgaacaac atcacacagt ccggcgtcac ggagtggagc aagcgtgaca agtccacgta
     6541 tgacccgacc gacgatatcg aagcctacgc gctgaacgcc agcggtgtgg tgaatatcat
     6601 cgtgtttgac ccgaaaggct gggcgctgtt ccgttccttc aaagccgtca aggagaagct
     6661 ggatacccgt cgcggctctc attccgagct ggagacagcg gtaaaagacc tgggcaaagc
     6721 ggtgtcctat aagggaatgt atggcgatgt ggccatcgtc gtgtattccg gacagtacgt
     6781 ggaaaacggc gtcaaaaaga acttcctgcc ggacaacacg atggtgctgg ggaacactca
     6841 ggctcgcggt ctgcgcacct atggctgcat tcaggatgcg gacgcacagc gcgaaggcat
     6901 taacgcctct gcccgttacc cgaaaaactg ggtgaccacc ggcgatccgg cgcgtgagtt
     6961 caccatgatt cagtcagcac cgctgatgct gctggctgat cctgatgagt tcgtgtccgt
     7021 tcaactggcg taatcatggc ccttcggggc cattttctct ctgtggagga gttcatgacg
     7081 aaagatgaac tgattgcccg tcttcaggag ctgggtgagc aattgaaccg cgatgtcagt
     7141 ttgacgggaa cgaaagaaga actggcgctt cgtgtggcag agctggaaga agagcttgat
     7201 gacacggatg aaactgccgg tcaggacacc cctctcagcc gggaaaatgt gctgaccggg
     7261 catgaaaatg aggtggtatc agcgcagccg gataccgtga ttcaggatac ggctgaactg
     7321 gtcacggtcg tggcactggt gacgctgcat actgatgcac ttcacgccac gcgggataaa
     7381 cctgtggcat ttgtgctgcc gggaacggcg tttcgtgtct ctgccggtgt ggcagccgaa
     7441 atgacagagc gcggcctggc cagaatgcaa taacgggagg cgctgtggct gatttcgata
     7501 acctgttcga tgctgccatt gcccgcgccg atgaaacgat acgcgggtac atgggaacgt
     7561 cagccaccat gacatccggt gagcagtcag gtgcggtgat acgtggtgtt tttgatgacc
     7621 ctgaaaatat cagctatgcc ggacagggcg tgcgcgttga aggctccagc ccgtccctgt
     7681 ttgtccggac tgatgaggtg cggcagctgc ggcgtggaga cacgctgacc atcggtgagg
     7741 aaaatttctg ggtagatcgg gtttcgccgg atgatggcgg aagttgtcat ctctggcttg
     7801 gacggggcgt accgcctgcc gttaaccgtc gccgctgaaa gggggatgta tggccataaa
     7861 aggtcttgag caggccgttg aaaacctcag ccgtatcagc aaaacggcgg tgcccggtgc
     7921 cgccgcaatg gccattaacc gcgttgcttc atccgcgata tcgcagtctg cgtcacaggt
     7981 tgcccgtgag acaaaggtac gccggaaact ggtaaaggaa agggccaggc tgaaaagggc
     8041 cacggtcaaa aatccgcagg ccagaatcaa agttaaccgg ggggatttgc ccgtaatcaa
     8101 gctgggtaat gcgcgggttg tcctgtcccg ccgcaggcgt cataaaaagg ggcagcgttc
     8161 atccctgaaa ggtggcggca gcgtgcttgt ggtgggtaac cgtcgtattc ccggcgcgtt
     8221 tattcagcaa ctgaaaaacg gccggtggca tgttatgcag cgtgtggccg ggaaaaaccg
     8281 ttaccccatt gatgtggtga aaatcccgat ggcggtgccg ctgaccacgg cgtttaaaca
     8341 gaatattgaa cggatacggc gtgaacgtct tccgaaagag ctgggctatg cgctgcagca
     8401 tcaactgaga atggtaataa agcgatgaaa catactgaac tccgtgcagc cgtactggat
     8461 gcactggaga agcatgacac cggggcgacg ctttttgatg gtcgccccgc tgtttttgat
     8521 gaggaagatt ttccggcaat tgccgtttat ctcaccggcg ctgaatacac gggcgaagag
     8581 ctggacagcg atacctggca ggcggagctg catattgaag ttttcctgcc tgctcaggtg
     8641 ccggattcag agctggattc gtggatggag tcccggattt atccggtgat gagcgatatc
     8701 ccggcactgt cagatttgat caccagtatg gtggccagcg gctatgacta ccggcgcgac
     8761 gatgatgcgg gcctgtggag ttcagccgat ctgacttatg tcattatcta tgaaatgtga
     8821 ggacgctatg cctgtaccaa atcctacaat gccggtgaaa ggtgccggga ccaccctgtg
     8881 ggtttataag gggagcggtg acccttatgc gaacccgctt tcagacgttg actggtcgcg
     8941 tctggcaaaa gttaaagacc tgacgcccgg cgaactgacc gctgagtcct atgacgacag
     9001 ctatctcgat gatgaagatg cagactggac tgcgaccggg caggggcaga aatctgccgg
     9061 agataccagc ttcacgctgg cgtggatgcc cggagagcag gggcagcagg cgctgctggc
     9121 gtggtttaat gaaggtgata cccgtgccta taaaatccgc ttcccgaacg gcacggtcga
     9181 tgtgttccgt ggctgggtca gcagtatcgg taaggcggtg acggcgaagg aggtgattac
     9241 ccgcacggtg aaggtcacca atgtgggacg tccgtcgatg gcagaagatc gcagcacggt
     9301 gacggcgacg accggcatga cggtgacacc cgccagtgct tccgtagtga aagggcagag
     9361 caccacgctg accgtggcat tccagccgga tggcgcaacc gacaagagct tccgtgcggt
     9421 gtctgcggat aaaacaaaag ccaccgtgtc ggtcagtggt atgaccatca ccgtgaaagg
     9481 tgttgctgca ggcaaggtca acattccggt cgtatctggt aatggtgagt ttgctgcggt
     9541 tgcagaaatc aacgtcaccg ccagttaatc cggagagtca gcgatgttcc tgaaaaccga
     9601 atcatttgaa cataacggtg tgaccgtcac gctttctgaa ctgtcagccc tgcagcgtat
     9661 tgagcatctc gacctgatga aacggcaggc agaacaggcg gagtcagaca gcaaccggaa
     9721 gtttactgtg gaagacgcca tcagaaccgg cgcttttgtg gttgcgatgt ccctgtggca
     9781 taaccatccg aagaagacgc agatgccgtc catgaatgaa gccgttaaac agattgagca
     9841 ggaagtgctt accacctggc ccacagaggc aatttctcat gctgaaaacg tggtgtaccg
     9901 gctgtccggt atgtatgagt ttgtggtgaa tgatgctcct gaacaggcag aggacgccgg
     9961 gcctgcagag cctgtttctg cgggaaagtg ttcgacggtg agctgagttt tgccctgaaa
    10021 ctggcgcgtg agatggggcg acccgactgg cgcgccatgc ttgccgggat gtcatccacg
    10081 gagtatgccg actggcaccg cttttacagt acccattatt ttcatgatgt tctgctggat
    10141 atgcactttt ccgggctgac gtacaccgtg ctcagcctgt ttttcagcga tccggagatg
    10201 catccgctgg atttcagtct gctgaaccgg cgcgaggctg acgaagagcc tgaagatgat
    10261 gtgctgatgc agaaagcggc agggcttgcc ggaggtgtcc gctttggccc ggacgggaat
    10321 gaagttatcc ccgcttcccc ggatgtggcg gacatgacgg aggatgacgt aatgctgatg
    10381 acagtatcag aagggatcgc aggaggagtc cggtatggct gaaccggtag gcgatctggt
    10441 cgttgatttg agtctggatg cggccagatt tgacgagcag atggccagag tcaggcgtca
    10501 tttttccggt acggaaagtg atgcgaaaaa aacagcggca gtcgttgaac agtcgctgag
    10561 ccgacaggcg ctggctgcac agaaagcggg gatttccgtc gggcagtata aagccgccat
    10621 gcgtatgctg cctgcacagt tcaccgacgt ggccacgcag cttgcagggg ggcagaatcc
    10681 ctggctgatc ctgctgcaac agggtggtca ggttaaggac tccttcggcg ggatgatccc
    10741 catgttcagg gggcttgccg gcgcgatcac cctgccgatg gtgggggcca cctcgctggc
    10801 ggtggcgacc ggtgcgctgg cgtatgcctg gtatcagggc aactcaaccc tgtccgattt
    10861 caacaaaacg ctggtccttt ccggtaatca ggcgggactg acggcagatc gtatgctggt
    10921 cctgtccaga gccgggcagg cggcagggct gacgtttaac cagaccagcg agtcactcag
    10981 cgcactggtt aaggcggggg taagcggtga ggctcagatt gcgtccatca gccagagtgt
    11041 ggcgcgtttc tcctctgcat ccggcgtgga ggtggacaag gtcgctgaag ccttcgggaa
    11101 gctgaccaca gacccgacgt cggggctgac ggcgatggca cgccagttcc ataacgtgac
    11161 ggcggagcag attgcgtatg ttgctcagtt gcagcgttcc ggcgatgaag ccggggcatt
    11221 gcaggcggcg aacgaggccg caacgaaagg gtttgatgac cagacccgcc gcctgaaaga
    11281 gaacatgggc acgctggaga cctgggcaga caggactgca cgtgcattca aatccatgtg
    11341 ggatgcggtg ctggatattg gtcgtcctga taccgcgcag gagatgctga ttaaggcaga
    11401 ggctgcgttt aagaaagcag acgacatctg gaatctgcgc aaggatgatt atttcgttaa
    11461 cgatgaagca cgggcgcgtt actgggatga tcgtgaaaaa aaacgcctgg agcgggatgc
    11521 agcccaaaag agggtagatc aacagcgtca acaggacaaa aatgcgcagc agcagagcga
    11581 taccgaagcg tcacggctga aatataccga agaggcgcag aaggcttacg aacggctgca
    11641 gacgccgctg gagaaatata ccgcccgtca ggaagaactg aacaaggcac tgaaagacgg
    11701 aaaaatcctg caggcagatt acaacacgct gatggcggcg gcgaaaaagg attatgaagc
    11761 gacgctgaaa aagccgaaac agtccggcgt gaaggtgtat gcgggcgatc gtcaggaaga
    11821 cagtgctcat gcggccctgc tgacgcttca ggcagaactc cggacgctgg agaagcatgc
    11881 cggagcgaat gagaaaatca gccagcagcg ccgggatttg tggaaggcag aaagtcagtt
    11941 cgcggtactg gaggaggcgg cacaacgtcg ccagctgtcc gcacaggaga aatccctgct
    12001 ggcgcataaa gacgagacgc tggagtacaa acgccagctg gctgcacttg gtgacaaggt
    12061 tacgtatcag gagcacctga atgcgctggc gcagcaggcg gataaattcg cacagcagca
    12121 acgggcaaaa cgggcagcca ttgatgcgaa aaaccggggg ctgactgacc ggcaggcagc
    12181 gcgggaagcc acggaacagc gcctgaagga acagtatggc gataatcctc tggcgctgaa
    12241 taacgtcatg tcagagcaga aaaagacctg ggcggctgaa gaccagcttc gcgggagctg
    12301 gatggcaggc ctgaagtccg gctggagtga gtgggaagag agcgccacgg acagtatgtc
    12361 acaggttaaa agtgcagcca cgcagacctt tgatggtatt gcacagaata tggcggcgat
    12421 gctgaccggc agtgaacaga actggcgcag cttcacccgc tccgtgctgt ccatgatgac
    12481 agaaattctg cttaagcagg caatggtggg gattgtcggg agtatcggca gcgccattgg
    12541 cggggctgtt ggtggcggcg catccgcgtc aggtggtaca gccattcagg ccgctgcggc
    12601 gaaattccat tttgcaactg ggggatttac gggaaccggc ggcaaatatg agccagcggg
    12661 gattgttcac cgtggtgaat ttgtcttcac gaaggaggca accagccgga ttggcgtggg
    12721 gaatctctac cggctgatgc gcggctatgc ggaaggtggt tatgtgggcg gtgccggaag
    12781 tccggcgcag atgcggcggg ctgaaggcat taattttaat cagaacaatc acgtggtgat
    12841 tcagaacgac ggtacgaatg gtctgccagg tccacagatg atgaaggccg tgtatgacat
    12901 ggcccgcaag ggtgcccgtg atgaaattca gacacagatg cgtgatggtg gcctgttctc
    12961 cggaggtgga cgatgaaaac cttccgctgg aaagtgaaac ccggtatgga tgtggcttcg
    13021 gccccttctg taagaaaggt gcgctttggt gatggctatt cccagcgagc gcctgccggg
    13081 ctgaatgcca acctgaaaac gtacagcgtg acgctttctg tcccccgtga ggaggccatg
    13141 gtactggagt cgtttctgga agagcacggg ggctggaaag cctttctgtg gacgccgcct
    13201 tatgagtggc ggcagataaa ggtgacctgc gcaaaatggt cgtcgcgggt cagtatgctg
    13261 cgtgttgagt tcagcgcaga gtttgaacag gtggtgaact gatgcaggat atccggcagg
    13321 aaacactgaa tgaatgcacc cgtgcggagc agtcggccag cgtggtgctc tgggaaatcg
    13381 atctgacaga ggtcggtgga gaacgttatt ttttctgtaa tgagcagaac gaaaaaggtg
    13441 agccggtcac ctggcagggg cgacagtatc agccgtatcc cattcagggg agtggttttg
    13501 aactgaatgg caaaggcacc agtacgcgcc ccacgctgat ggtttctaac ctgtacggta
    13561 tggtcaccgg gatggcggaa gatctacaga gtctggtcgg cggaacggtg gtccggcgta
    13621 aggtttacgc ccgttttctg gatgcggtga acttcgtcaa cggaaacagt gacgccgatc
    13681 cggagcagga ggtgatcagc cgctggcgca ttgagcagtg cagcgaactg agcgcggtga
    13741 gtgcctcttt tgtactgtcc acgccgacgg aaacagatgg cgctgttttt ccgggacgta
    13801 tcatgctggc caacacctgc acctggacct atcgcggcga tgagtgcggt tatcacggtc
    13861 cggcggtcgc ggatgaatat gaccagccaa cgtccgacat cacgaaggat aaatgcagca
    13921 aatgcctgag tggctgtaag tttcgcaata acgtcggcaa ctttggcggc ttcctttcca
    13981 ttaacaaact ttcgcagtaa atcccatgac agagacagaa tcagcgattc tggcgcacgc
    14041 ccggcgatgt gctccagcgg agtcgtgcgg cttcgtggta agaacgccgg agggggaaag
    14101 atatttcccc tgcgtgaata tctccggtga gccggaggcg tatttccgta tgtcgccgga
    14161 agactggctg caggcagaga tgcagggtga gattgtggca ctggtccaca gccaccccgg
    14221 tggtctgccc tggctgagtg aggccgaccg gcggctgcag gtgcagagtg atttgccgtg
    14281 gtggctggtc tgccgggggg cgattcacaa gttccgctgt gtgccacatc ttaccgggcg
    14341 gcgctttgag cacggggtga cggactgtta cacgctgttc cgggatgctt accatctggc
    14401 ggggattgag atgccggatt ttcatcgtga ggatgactgg tggcgtcacg gtcagaatct
    14461 ctatctggat aatctggagg ccacagggct gtatcaggtg ccgttgtcag cggcgcagcc
    14521 gggcgatgtg ctgctgtgct gttttggttc atcggtgccg aatcatgccg ccatttactg
    14581 tggtgacggc gagctgctgc accatattcc tgaacaactg agcaaacgag agaggtatac
    14641 cgacaaatgg cagcgacgca cacactccct ctggcgtcac cgggcatggc acgcatctgc
    14701 ctttacgggg atttgcaacg atttggccgc cgcatcgacc ttcgtgtgaa aacgggtgcc
    14761 gaagccatcc gggcgatggc cacacagctc cctgcgtttc gtcagaagct gagcgacggc
    14821 tggtatcagg tgcgtattgc cgggcaggat gttagcacgt ccggattaac ggcgcagtta
    14881 catgaggctc tgcctgacgg cgctgtgatt catattgttc ccagagtcgc cggggccaag
    14941 tcaggtggtg tattccagat tgtcctggga gcagccgcca ttgccggatc attctttacc
    15001 gccggagcca cccttgcagc atggggggct gccattggtg ccggtggtat gaccggcatt
    15061 ctgttttctc tcggtgccag tatggtgctc ggcggtgtgg ctcagatgct ggcaccgaaa
    15121 gccagaactc cccgcacaca gacaacggat aacggtaagc agaacaccta tttctcctca
    15181 ctggataaca tggttgccca gggcaatgtt atgcctgttc tgtatggtga aatgcgcgtg
    15241 gggtcacgtg tggtatctca ggagatcagc acggcagacg aaggggacgg tggtcaggtt
    15301 gtggtgattg gtcgctgatg caaaatgttt tatgtgaaat cgcctccggg cggttttatc
    15361 gtttatggag catgacgaat gggtaaaggc agcagtaagg ggcatacccc gcgcgaagcg
    15421 aaggacaacc tgaagtccac gcagctgctg agtgtgatcg atgccatcag cgaagggccg
    15481 gttgaaggtc cggtggatgg attaaaaagc gtgctgctga acagtacgcc ggtgctggac
    15541 agtgagggga ataccaacat ctccggcgtc acggtggtgt tccgggcagg tgagcaggag
    15601 cagtcaccgc cggagggatt tgaatcctcc ggctccgaga cggtgctggg tacggaagtg
    15661 aaatatgaca cgccgatcac ccggaccatc acgtcggcaa acattgaccg actgcgtttt
    15721 accttcggtg tacaggcact ggtggaaacc acctcaaagg gagacaggaa tccgtctgaa
    15781 gttcgcctgc tggttcagat acagcgtaac ggtggctggg tgacggaaaa agacatcacc
    15841 attaagggca aaaccacctc gcagtatctg gcctcggtgg tggtggataa cctgccgccg
    15901 cgcccgttta atatccggat gcgcagaatg acgccggaca gcaccacaga ccagctgcag
    15961 aacaaaacgc tctggtcgtc atacaccgaa atcatcgatg tgaaacagtg ctacccgaac
    16021 acggcactgg tcggcgtaca ggtggattcg gagcagttcg gcagccagca ggtgagccgt
    16081 aattatcatc ttcgcgggcg cattctgcag gtgccgtcga actataatcc gcagacgcgg
    16141 caatacagcg gtatctggga cggaacgtta aaaccggcat acagcaacaa catggcctgg
    16201 tgtctgtggg atatgctgac ccatccgcgc tatggcatgg ggaaacgtct tggtgcggca
    16261 gatgtggata aatgggcgct gtatgtcatc ggccagtact gtgaccagtc ggtgccggac
    16321 ggctttggca gcacggagcc gcgcatcacc tgtaatgcct ggctgaccac acagcgcaag
    16381 gcgtgggatg tgctcagtga tttctgctcg gcgatgcgct gtatgccggt atggaacggg
    16441 cagacgctga cgttcgtgca ggaccgacag tcggataagg tgtgggccta taaccgcagt
    16501 aatgtggtga tgccggatga tggcgcgccg ttccgctaca gtttcagcgc cctgaaagac
    16561 cgccataatg ccgttgaggt gaactggatt gacccggata acggctggga gacggcgaca
    16621 gagcttgtgg aggatacgca ggccattgtc cgttacggtc gtaacgtcac gaagatggat
    16681 gcctttggct gtaccagccg ggggcaggca caccgcgccg ggctgtggct gattaaaacg
    16741 gaactgctgg agacgcagac cgtggatttc agcgtgggtg ctgaagggct tcgtcatgta
    16801 ccgggcgatg tcattgaaat ctgcgatgat gactatgccg gtatccgcac cggcgggcgc
    16861 gtgctggcgg tgaacagcca gacccggacg ctgacgctcg accgtgaaat cacgctgcca
    16921 tcctccggca ccacgctgat aagcctggtt gacgggcagg ggaatccggt cagcgtggag
    16981 gtccagtccg tcaccgacgg cgtgaaggta aaagtgagcc gtgttcctga cggtgttgct
    17041 gaatacagcg tgtgggggct gaagctgccg acgctgcacc agcgattgtt ccgctgcgtg
    17101 agtatccgtg agaacgacga cggcacgtat gccatcaccg ccgtgcagca tgtgccggaa
    17161 aaagaagcca tcgtggataa cggggcgcac tttgacggcg accagagcgg cacggtgaat
    17221 ggtgtcacgc cgccagcggt gcagcacctg actgccgaag tcaccgcaga cagcggggaa
    17281 tatcaggtgc tggcgcgctg ggacacgccg aaggtggtga agggcgtgag cttcctgctc
    17341 cgtctgaccg taacagcgga tgacggcagt gagcggctgg tcagcacggc ccggacgacg
    17401 gaaaccactt accgcttcac acaactggcg ctggggaact acaggctgac agtccgggca
    17461 gtaaatgcgt gggggcagca gggcgatccg gcgtcggtat cgttccggat tgccgcaccg
    17521 gcagcgctgt cgcggattga gctgacgccg ggctattttc agataaccgc cacgccgcat
    17581 cttgcggttt atgatccgac ggtacagttt gagttctggt tctcggaaaa gcggattgcg
    17641 gatatcaggc aggttgaaac cacagcacgc tatcttggca cggcgctgta ctggatagcc
    17701 gccagtatca atatcaaacc gggccatgat tattactttt atatccgcag tgtgaacacc
    17761 gttggcaaat cggcattcgt ggaggctgtt ggtcagccga gtgatgatgc atccggttat
    17821 ctggattttt tcaaaggcga gatagggaaa acccatctgg ctcaggagct gtggacgcag
    17881 attgataacg gtcagcttgc gcctgacctg gctgaaatca ggacgtccat tacggatgtc
    17941 agcaatgaaa tcacgcagac cgtcaataag aaactggaag accagagtgc ggcaattcag
    18001 cagatacaga aggttcaggt tgatacaaat aataacctga acagcatgtg ggctgtgaag
    18061 ctgcagcaga tgcaggacgg acgcctttat atcgcgggta ttggtgccgg tattgagaac
    18121 acccctgacg gcatgcagag tcaggtgctg ctggcggcgg acaggattgc gatggttaat
    18181 cctgcgaatg gcaacacaaa accgatgttt gttggtcagg gcgatcagat attcatgaac
    18241 gacgtgttcc tgaaacgcct gacggccccc accattacca gcggtggaaa tccatcggca
    18301 ttttccctga ctccggacgg aaagctgacc gctaaaaatg cggatatcag tggcagtgtg
    18361 aatgcgaacg ccgggacgct caacaatgtc acggtaaatg aaaactgtac gattaagggc
    18421 atgctggagg cgactcaggt cagaggtgac ttcgttaaag ctgtatccaa atcatttccg
    18481 aaacaggctg gtacgtgggg taacacggaa acaccaaacg ggacggttac agtcaccata
    18541 agcgatgatc ataactttga ccgccagatt attattccgc ccattatttt taacggtata
    18601 gcgtatgacg atccggggag cggaaataac ccaggaggca cgcgatacac gggttatggt
    18661 tttgaagttc gcaaaaacgg cgtattaatc gcatccagag aaactaaagg tgccattccc
    18721 ggtagttaca gtgcagttat tgatatgcct agtggtggtg gtagcgtcac tctggagttt
    18781 aagattttcc agaaaggcaa tcagggggca ggcaatatca ccgactgtac ggtgattgtg
    18841 accaaaaaag cggcttccgg catcagtatt cgttgaaata tttataaccc caataaatgg
    18901 cgtcaggaat gacgcctttt ttattgcaga aaagcgagag gtaattatgc gtaaagtttg
    18961 tgcagccatt ttgtccgcag ccatttgcct ggccgtatcc ggtgcgcctg catgggcgtc
    19021 tgaacatcag tccacgctga gcgcggggta tcttcatgcc cggaccaacg ttcccggcag
    19081 tgatgatctg aacgggatta acgtgaaata ccgttatgag tttacggata cgctggggct
    19141 ggtgacgtca ttcagctatg caggagacaa gaatcgccag ctgacccgtt acagcgatac
    19201 ccgctggcat gaagattccg tgcgtaaccg ctggttcagc gtgatggcgg ggccgtctgt
    19261 gcgcgtgaat gaatggttca gcgcgtatgc gatggcgggt gtggcttaca gccgtgtgtc
    19321 gactttttcc ggggattata tccgcgtaac tgacaataag gggaaaacgc acgatgtgct
    19381 gaccggaagt gatgacggtc gccacagcaa cacgtctctg gcgcactgac ggtttcatcg
    19441 tgggtgtcgg ttataaattc tgattagcca ggtaacacag tgttatgaca gcccgccggt
    19501 tcaggcgggc ttttttgtgg ggtgaatatg gcagtaaaga tttcaggtgt actgaaagac
    19561 ggcacaggaa aaccggtaca gaactgcaca atccagctga aagcaaaacg taacagcacc
    19621 acggtggtgg tgaacacgct ggcctcagaa aatccggatg aagccgggcg ttacagcatg
    19681 gacgttgagt acggtcagta cagcgttatt ctgttggtgg aaggattccc gccgtcacat
    19741 gccgggacca tcaccgtgta tgaagattcc cgacccggta cgctgaatga ttttctcggt
    19801 gccatgacgg aggatgatgc ccgtccggag gcactgcgcc gttttgagct gatggtggaa
    19861 gaggtggcgc gtaacgcgtc cgcggtggca cagaacacgg cagccgcgaa gaaatcagcc
    19921 agcgatgcca gcacatcagc ccgtgaggcg gcaacccatg cgactgatgc tgcagattca
    19981 gcacgtgcag ccagcacgtc agccggacag gccgcttcgt cggctcagtc agcgtcttcc
    20041 agcgcaggaa cggcatcaac aaaggccact gaagcctcaa aaagtgctgc cgctgcagag
    20101 tcctcaaaaa gcgcggcggc taccagtgcc ggtgcggcga aaacgttaga aacgaatgcg
    20161 gcagcgtcac aacaatcagc agccacttct gcatccaccg cgaccacgaa agcgtcagaa
    20221 gctgccacct cagcccggga tgcgtcggct tcaaaagagg cggcaaaatc atcagaaacg
    20281 aacgcatcat caagcgccag tagcgcagct tcctcggcga cggcggctgc aaattctgcg
    20341 aaggcggcca aaacgtccga gacgaacgcc aggtcttctg aaacggcagc gggacagagt
    20401 gcctcagctg cggcaggctc aaaaacagcg gctgcgtcgt ctgccagcgc cgcgtcaaca
    20461 agtgccgggc aggcctcagc cagtgccacc gccgccggaa aatcggcaga aagcgccgca
    20521 tcatccgctt caacagccac aacgaaggct ggcgaagcca ctgagcaggc cacggcagca
    20581 gcgaggtcag cttccgcagc gaagacatcc gaaacgaacg cgaaagcgtc ggaaacccgt
    20641 gcagaatcct caaaaacggc tgccgcatcg tccgccagtt cggcggcgtc atcggcatca
    20701 tcggcgtctg cttcaaaaga tgaggcgacc agacaggcgt cagcagcgaa gggcagcgcc
    20761 acgacggcat ccacgaaggc gacagaggct gctggtagtg cgacggcagc agctcagagc
    20821 aaaagtacgg cggaatctgc agcaacgcgc gctgagacag cggcaaaacg ggcagaggat
    20881 attgcatccg ccgtggcgct tgaggatgcg agcacgacga aaaaggggat agtacagctc
    20941 agtagtgcga ctaacagcac ttccgagtca ctggcggcaa caccaaaagc cgttaaggcc
    21001 gcgtatgacc tggctaacgg gaaatacacc gcacaggatg caacgacagc acagaaaggg
    21061 ataatccagc taagcagcgc gaccaacagc acgtctgaaa cgctggcggc aacgccaaag
    21121 gcagtaaaag cagccaatga caatgctgag aaacgtctgc agaaagatca gaacggtgcg
    21181 gatatccctg gcaaagacac ctttacgaaa aatattggtg cctgccgtgc cttcggtggg
    21241 tcagtaagca caacaacagg aaactggacg actgcacagt ttatcgagtg gctggattct
    21301 cagggagcat ttaaccatcc atactggatg tgcaagggtt cctggtctta tggcaataat
    21361 aaaatcatta ctgatactgg ctgcggtaat attcatctcg ccggagctgt cattgaagta
    21421 atggggataa agtcagcgat gacgatccgc attaccactc cgaccacatc cagcggtggc
    21481 ggaacaacca acgcccagtt tacctatatt aatcacggaa cagattattc acctggctgg
    21541 cgaagggact ataactccag aaataagcca acggcatcag agatcggggc gctaccgtca
    21601 ggtggaacag cagtatcatc agttaatctg gcttcaaaag gtcgggtagc cgcgctgaca
    21661 gacaatacgc agggggcagc aggtcttgag ttatacgagg tgtataacaa cggatatcca
    21721 acagcgtatg gaaatatcat tcacctgaaa gggatgacag ccgttggcga aggtgagtta
    21781 ctcatcggct ggagtggtac aagcggtgct catgctccgg catttattcg ttcacgacgg
    21841 gatacgaccg acgcaaactg gtcgccgtgg gcgcagcttt acacctcggc tcatcctcct
    21901 gcagagtttt atccagtcgg tgcaccaatc ccgtggccat cagataccgt tccgtctggt
    21961 tatgccctga tgcaggggca gacttttgac aaatctgctt acccgaaact tgcagccgct
    22021 tatccgtcag gcgtgatccc tgatatgcgt ggctggacga ttaagggcaa gcccgccagt
    22081 ggtcgtgccg tattgtctca ggaacaggat ggcattaaat cgcacaccca cagcgccagc
    22141 gcatccagta cggatttggg gacgaaaacc acatcgtcgt ttgattacgg cactaaatcc
    22201 acgaataaca ccggggcgca tacgcacagt ctgagcggct ctacaagttc agctggggcc
    22261 caccaacata gtcagactgg gcccagaacg aatagcggga gtcaacctac tggaatgttt
    22321 ccggcaggga gtactcaagt tagcgggact aatcaggttg gtatcagtgg ctcattaacc
    22381 agtggcacct cccaatgggt aggtaaatca tcttcagaag gaaaccatac acactcactg
    22441 tccggcacag cagcatctgc gggtgcacac gcgcatactg tcggtattgg tgctcatacg
    22501 cactctgttg cgattggttc acatggacac accatcaccg ttaacgctgc tggtaacgcg
    22561 gaaaacaccg tcaaaaacat cgcatttaac tatattgtga ggctcgcata atggcattca
    22621 gaatgagtga acaagcacgg accataaaaa tttataacct gctggccgga actaatgaat
    22681 ttattggtga aggtgacgca tatattccgc ctcatacagg tctgcctgca aacagtaccg
    22741 atattgcacc gccagatatt ccggctggtt tcgtggctgt tttcaacagt gatgagtcat
    22801 cgtggcatct cgttgaagat catcggggta aaacggttta tgacgtggct tccggcgacg
    22861 cgttatttat ttctgaactc ggctcattac cggaaaatgt cacctggtta tccccggaag
    22921 gggagtttca gaagtggaac ggcacagcct gggtgaagga tacggaagca gaaaaactgt
    22981 tccggatccg ggaggcggaa gaaacaaaaa acagcctgat gcaggtagcc agtgagcata
    23041 tagcgccgct tcaggatgct gtggatctgg agatcgcaac ggagaaagaa accttgttgc
    23101 tggaagcctg gaaaaagtat cgggtgttgc tgaaccgtgt tgatacatca actgcaccgg
    23161 atattgaatg gccagtagca cctatagggt aaattcgtaa tgattaccta aatacgttat
    23221 tcttttgtta aaaaagtgat tttgctatag gtagggagta tgttcttgga ttaagaataa
    23281 aaaatcgcca tgaaagagct tcatggcgag tacataaacg tacaaaatgt gatgaaagta
    23341 gagtggcgta agccactata ttaagatatg acaatagcga agaaaagtaa ataacatttt
    23401 atggacgttt aattgttaat tataaaaact agagtgcccg atattgttta tcttgcttta
    23461 aaataatgta ctcaaaccaa atttttgagc taaaagctca aataatataa atgcaataat
    23521 aatgtttaat aagacaatta gcttgctttg tattttcatt tcggaatact gttaagtaac
    23581 tttgatgacc ctaacaatag atcggaattt tcttagagtc aatggtcact gtttttgctt
    23641 gcaaagcaaa tgttttggtg gttataagtt gtcttaatat agtcacgcat gcaacttgtt
    23701 ccatgcggag attaaagatt ggaatataac gcgaagatgg aattaaatta catttgcaat
    23761 aatcaattta ttattactca ataattaccg atattattat tgtaaattaa tcattagagt
    23821 gcaaaaagaa atgtaacggc aaaataagag gacgatagct gagaaaaaag cgtgctttcc
    23881 caatctgggg caaatcacgt ctgaatgaca cctgaaaaca ggtggtagcc tcaagaa
//
LOCUS       DAKISA010000064        23835 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.064, whole genome shotgun sequence.
ACCESSION   DAKISA010000064 DAKISA010000000
VERSION     DAKISA010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23835
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.064"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..885)
                     /locus_tag="OVW45_002602"
     CDS             complement(<1..885)
                     /locus_tag="OVW45_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCV5527015.1"
                     /translation="MDKKLLALLILASLSPAEATLTEIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQ"
     gene            complement(977..1477)
                     /locus_tag="OVW45_002603"
     CDS             complement(977..1477)
                     /locus_tag="OVW45_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCV5527016.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            complement(1507..2223)
                     /locus_tag="OVW45_002604"
     CDS             complement(1507..2223)
                     /locus_tag="OVW45_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCV5527017.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQRLKILTIAYCKNNSKENCKIQTVNKNIFLGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            2519..3295
                     /gene="exuR"
                     /locus_tag="OVW45_002605"
     CDS             2519..3295
                     /gene="exuR"
                     /locus_tag="OVW45_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCV5527018.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            3640..4302
                     /locus_tag="OVW45_002606"
     CDS             3640..4302
                     /locus_tag="OVW45_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCV5527019.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPIFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            4306..4689
                     /gene="mzrA"
                     /locus_tag="OVW45_002607"
     CDS             4306..4689
                     /gene="mzrA"
                     /locus_tag="OVW45_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCV5527020.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            4836..5204
                     /locus_tag="OVW45_002608"
     CDS             4836..5204
                     /locus_tag="OVW45_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HCV5527021.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            5242..5547
                     /locus_tag="OVW45_002609"
     CDS             5242..5547
                     /locus_tag="OVW45_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCV5527022.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            5550..5954
                     /locus_tag="OVW45_002610"
     CDS             5550..5954
                     /locus_tag="OVW45_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCV5527023.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            5944..6243
                     /locus_tag="OVW45_002611"
     CDS             5944..6243
                     /locus_tag="OVW45_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCV5527024.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            6429..6821
                     /locus_tag="OVW45_002612"
     CDS             6429..6821
                     /locus_tag="OVW45_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCV5527025.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            6891..7877
                     /gene="yqjG"
                     /locus_tag="OVW45_002613"
     CDS             6891..7877
                     /gene="yqjG"
                     /locus_tag="OVW45_002613"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCV5527026.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYISLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESTEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            8170..8535
                     /locus_tag="OVW45_002614"
     CDS             8170..8535
                     /locus_tag="OVW45_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCV5527027.1"
                     /translation="MDWYLKVIKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            8777..9133
                     /locus_tag="OVW45_002615"
     CDS             8777..9133
                     /locus_tag="OVW45_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCV5527028.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
     gene            complement(9184..10080)
                     /gene="yhaJ"
                     /locus_tag="OVW45_002616"
                     /pseudo
     CDS             complement(9184..10080)
                     /gene="yhaJ"
                     /locus_tag="OVW45_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
     gene            10185..10886
                     /locus_tag="OVW45_002617"
     CDS             10185..10886
                     /locus_tag="OVW45_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCV5527029.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            10909..11073
                     /locus_tag="OVW45_002618"
     CDS             10909..11073
                     /locus_tag="OVW45_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5527030.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(11207..12517)
                     /locus_tag="OVW45_002619"
     CDS             complement(11207..12517)
                     /locus_tag="OVW45_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCV5527031.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(12545..13876)
                     /locus_tag="OVW45_002620"
     CDS             complement(12545..13876)
                     /locus_tag="OVW45_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCV5527032.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(14151..15515)
                     /gene="tdcG"
                     /locus_tag="OVW45_002621"
     CDS             complement(14151..15515)
                     /gene="tdcG"
                     /locus_tag="OVW45_002621"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCV5527033.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAAWMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(15587..15976)
                     /locus_tag="OVW45_002622"
     CDS             complement(15587..15976)
                     /locus_tag="OVW45_002622"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCV5527034.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(15990..18284)
                     /gene="tdcE"
                     /locus_tag="OVW45_002623"
     CDS             complement(15990..18284)
                     /gene="tdcE"
                     /locus_tag="OVW45_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCV5527035.1"
                     /translation="MKIDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(18318..19526)
                     /gene="tdcD"
                     /locus_tag="OVW45_002624"
     CDS             complement(18318..19526)
                     /gene="tdcD"
                     /locus_tag="OVW45_002624"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCV5527036.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVVIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(19552..20883)
                     /gene="tdcC"
                     /locus_tag="OVW45_002625"
     CDS             complement(19552..20883)
                     /gene="tdcC"
                     /locus_tag="OVW45_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCV5527037.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(20905..21894)
                     /gene="tdcB"
                     /locus_tag="OVW45_002626"
     CDS             complement(20905..21894)
                     /gene="tdcB"
                     /locus_tag="OVW45_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCV5527038.1"
                     /translation="MHITYDLPIAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(21993..22931)
                     /gene="tdcA"
                     /locus_tag="OVW45_002627"
     CDS             complement(21993..22931)
                     /gene="tdcA"
                     /locus_tag="OVW45_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCV5527039.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            23120..23464
                     /gene="tdcR"
                     /locus_tag="OVW45_002628"
     CDS             23120..23464
                     /gene="tdcR"
                     /locus_tag="OVW45_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCV5527040.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
BASE COUNT         6273 a         6033 c         5500 g         6029 t
ORIGIN      
        1 ttgctcatta cgataagcgt caacacgaga attgcgcgaa agcagaacca ttactggggt
       61 tccttgctgg ctttgcgcct ggtttaaata gctcagtgtc gacccgatac gcatcccatc
      121 aattgcaccg agtggcagaa agttaaaggt aaagttcccg ccttgtgcat taaatagtgt
      181 gcggttgtcc atgcgacccg cctgcacata ataacgacgt aaaaaatcat aacgataata
      241 gagatcgctg acgtcagcat tactgtcact gacatcatct gcatcataac cgttgaaatt
      301 ccagtgtgca ccaatataac tattttctgt tattcccagc gcaccgtttc cctgaataga
      361 caacgattgg taatcatcct gtgccagaac attgatgtct tgctgatgta taaaagcatt
      421 aacagtattt ttgtctggat ttaaatataa tgacttcgca tcgaaagctg aattccattg
      481 cggattaata aacaacgttg ccgaactttc ttcatcatta tagattattt ccagcgtatc
      541 ggtattcaga aaaccacacc ctgagtcagt acgtacataa ccgcaagcca attcaccgtg
      601 gcgagctagc ggctgcgata atttttcttt cactatatgc gcgattttct gatcgtctac
      661 atccagttcc agcttgttat atagacttgc tggatcaaga aatgttacgg tatcaagatt
      721 aaccattgca taatatttac cgagactttt ccctgaaaaa taaacctcga tatactcctg
      781 ctgtccctga gcaataacct caaaccctgc gggaatttcg gttaatgtcg cctctgccgg
      841 gctgagactc gccaggatca aaagagccag taatttttta tccatggcaa tcctgaaatc
      901 cataacaaat actaaaacag ttaaaataag ggagggctac ccctcccttt gttacgtgtt
      961 aattctgtta tctggattag atatcctgag acagataaat gcttactaca ccacggtaga
     1021 tgcctgtctc cagaacacct ttggtggttt gagcaaacat caggttttta gccagagaac
     1081 cgttagttaa cgcatcggaa gcaaagattt tggttgcggt aaaagtggtt gccgcatcag
     1141 ctttaatttc ttcaccgccc caggttactg tcagcggaac aatcttgcta gcatccaggc
     1201 tttgaaccag ttgcgccggg gaaactaact gcatttttac atctttggta acgtcgttag
     1261 accagatttt ggtcatcaac tggtaagatt gcagaccctg ccccggcaga tattgcattt
     1321 ctactgcttt cggcaacgcg gtgttatcgg tctgtgtcat atccagagcg gcatcaacgt
     1381 tggccgttac agtaatatcc ttttgtactg ccaatgcgga gcctgcaaca gaaaacatcg
     1441 ctgcgaccag aagagatttt gcaaatacct ttttcattac tattccttcg tttattacaa
     1501 tgataattaa tggaccgcca gttcaaactc gccattatct ttggtgatcc agttattata
     1561 tttgacaacg attttgtcgt agccagatat gctttcaaga tttctttcct gaccaaggaa
     1621 gatgttttta tttacggtct gtatcttaca attttctttg ctattatttt tacaataagc
     1681 gatcgttaaa atctttaagc gttgattccc tgcattaact aatttttggg cagcatttac
     1741 ttctaactta gcaataggtt gctgaggtgg aacactaaca acaatccccc aaattaagtt
     1801 aactgttaac tgcatagaga gatctttttt atctgtggca acgtcatcaa tactgggaac
     1861 cgcttcaaat ttaacgcgat aatttttttc tttttgctct ggttccattg caacaaagcg
     1921 cacagttttg ctactaccgg ctggtaaagc gaatttaggt ggcataacaa ccagatgatt
     1981 tgcatcaccg gatttaatct caacttcatt ttcctgaggc gtcgatggat tatcgatacg
     2041 gaatactgtc gccttgatgt actgaacttc attactttta gatattacgc gaacgttgcc
     2101 ttcaccctga ctattaatgg aaacagccat aggataaaca gtcatatttg actgtgccaa
     2161 aaaaggagcg aacacgaata aaagcggcgt aagccgtgat aagagattaa ttttcgccac
     2221 catataaatt tgatcttaca atattgtttc caaaaagttg gtgtattaga gatgtagatt
     2281 tattgtaagt caatataaaa aagctttttt agttaatatt aaatttaaag gctgggaatt
     2341 aacttactga aatttagtag ctttcacaat gacgatactt attttttagg aatttataaa
     2401 tattcacaat attaattcaa tacattaatt caatcaccaa atttatatat gaccaatggc
     2461 atacccatga ataaagtggt atgataactt cgtccgcatt ccactccggg agacgcatat
     2521 ggaaatcact gaaccacgcc gtttgtatca acaacttgcc gctgacctga aagagcgcat
     2581 tgaacagggc gtctatctgg tgggtgataa actgcctgcc gaacgcttta ttgccgatga
     2641 aaagaacgtc agccgaacgg tagttcgtga agccatcatc atgctggaag ttgaaggcta
     2701 tgtggaggtg cgtaaaggtt caggtattca tgtggtttcc aaccagccgc gccatcagca
     2761 ggcggctgac aataatatgg aattcgccaa ttacggtccg tttgagttgc ttcaggctcg
     2821 ccagctcatc gaaagtaata ttgccgagtt cgcggcaact caggtaacga aacaagacat
     2881 catgaaactg atggccattc aggaacaggc gcgcggcgaa caatgcttcc gtgattccga
     2941 gtgggattta cagttccaca ttcaggtagc cctggcgacg cagaactccg ccctggcggc
     3001 catcgttgaa aaaatgtgga cccagcgtag ccataacccg tactggaaaa aactacacga
     3061 acacattgat tcccgtaccg ttgataactg gtgtgatgac cacgatcaaa ttctcaaggc
     3121 gttaattcgc aaagatcccc atgctgctaa gctggcaatg tggcagcatc tggaaaacac
     3181 caaaatcatg ttatttaacg aaaccagcga cgatttcgag ttcaatgccg accgctatct
     3241 attcgccgaa aacccggtag tgcatctcga tacggccacc agtggcagta aatgaaactc
     3301 ctgtccgaca ggcgctgcga cgcgcctgtt cgcttcgtcg gtaagcgaaa agtataaagt
     3361 gtcagcctgt gtaaatcctc tcgccaccct cccctgcatt cagcaaaatc agactccacg
     3421 gacatgtaat tttgataacg aacaacgttg acctttgtta caattagatt caatttgaat
     3481 ttatgttttt gaatgctttc ttatctcacg atttaacagg gaatagttca ggctgtgttg
     3541 atgtatcaaa cccgcagaac ataccaaaac agcaataaca ttgcggtagt gcatctttta
     3601 aaaccagcgt ggcgttaacc gattcaccag gaataatgaa tggaactttt gacccaattg
     3661 ctgcaagccc tgtgggcgca ggattttgaa accctggcca atccatcgat gattggcatg
     3721 ttgtattttg tcttgtttgt aattttgttc cttgaaaacg gcttgcttcc agcggccttt
     3781 ttaccgggcg acagtttact ggtattggtc ggcgtgttga ttgcgaaagg cgcgatgggt
     3841 tacccgcaaa cgattctgct gctgaccgtt gccgccagcc tcggctgctg ggtcagctat
     3901 attcaggggc gatggctggg caatacccgc accgtacaaa actggctatc tcatttaccc
     3961 gcgcattatc atcaacgcgc acaccatctt tttcataaac acggtttatc ggcgctgtta
     4021 attggtcgct ttattgcgtt tgtcagaaca ctgctgccga cgattgccgg gttatcaggg
     4081 ctgaataacg cgcgctttca gtttttcaac tggatgagcg gtctgctgtg ggtattgatc
     4141 ctgacaactc tgggttacat gctcggcaaa acgccgatat ttttaaagta cgaggaccag
     4201 ctgatgtcat gcctgatgct gctcccggtg gtgctgctgg tgtttggcct ggcaggttct
     4261 ctggtcgtgt tatggaaaaa gaaatatgga aatcgggggt aagggatgca aatacctcgc
     4321 atgtcgcttc gccagctagc ctggtccggc actgttttac ttctggtcgg cacgctgttg
     4381 ctggcctggt ccgcggttcg ccagcaagag tctacgctgg cgattcgtgc cgttcatcaa
     4441 ggcacaacga tgccagacgg tttttcgatc tggcatcacc ttgacgctca tggcattcct
     4501 ttcaaaagta tcacccccaa aaacgacact ctgttaatta catttgactc cagcgaccag
     4561 agcgccgccg caaaagcggt cctcgacaga acattgcccc atggctacat cattgcgcag
     4621 caggacaata acagtcaggc tatgcagtgg ctgacccggt tacgggataa ctctcatcgc
     4681 ttcggataac ttcctgttat ccgaaacata tcactcactt tggtgatttc accgtaactg
     4741 tctatgatta atgaggcggt ggttaacact accgcttcgt tgtctggaca atcgttcctt
     4801 tgtaataggt ccgaatcaca atggaaggtt caagaatgaa ataccgcatc gctttagctg
     4861 tttctctctt tgctcttagt gccggtagtt atgccactac cctgtgtcag gaaaaggagc
     4921 aaaatatcct taaggagatc agctatgccg aaaaacacca aaaccagaat cgtattgacg
     4981 gtctgaataa agccctgagt gaagtccggg ccaactgttc agatagccag ctgcgtgccg
     5041 atcatcacaa gaaaatcgca aagcagaaag atgaggtggc ggaacgccag caagatttag
     5101 ccgaggcgaa gcaaaaaggc gatgccgata agattgccaa acgcgaacgg aaactggcag
     5161 aagcgcagga agagctgaaa aagctggaag cgcgcgacta ctaactcaca atagtcacta
     5221 cttactcacc tggagaaaac tatgtcgaaa gaacacacta cggaacatct gcgtgctgag
     5281 ttgaaatccc tttccgatac gctggaagag gtgcttagct catctggcga gaagtcgaaa
     5341 gaagagttga gtaagattcg tagcaaagcg gagcaggcac tgaaacagag ccgttatcgc
     5401 ctgggtgaaa ccggtgatgc catcgccaaa caaacccgtg tcgcggcggc gcgtgctgat
     5461 gagtatgtgc gtgaaaatcc gtggaccggc gtgggaattg gcgctgcaat cggtgtagtg
     5521 ctcggcgttc tgctgtcgcg tcgttaatta tggcggacac tcatcacgcg caagggcccg
     5581 gtaaaagcgt tctgggcatc gggcagcgaa ttgtttccat catggttgaa atggtggaaa
     5641 cacgtctgcg gctggcggtg gtggagctgg aagaggaaaa agctaatctc tttcaacttt
     5701 tactgatgct gggcctgacg atgcttttcg ctgcatttgg tcttatgagc ctgatggtgc
     5761 taattatttg ggcggttgat ccgcaatatc gcctgaatgc gatgattgcc accaccgtgg
     5821 tgttgctgct actggcactg attggcggta tctggacgtt acgtaaatcg cgtaaatcca
     5881 ccttgctgcg ccatacacgc catgagctgg caaacgatcg gcaactgctc gaggaggagt
     5941 cccgtgagca gtaaagtcga acgtgaacga cgtaaggcgc aactgcttag ccagatccag
     6001 caacaacggc tggatctttc cgccagtcgt cgtgaatggc tggaggcaac aggcgcttac
     6061 gatcgtcgct ggaatatgct gctaagtctg cgctcctggg cgctggttgg cagtagcgtg
     6121 atggcgatct ggacgattcg ccatcctaat atgctggtcc gctgggccag acgcggtttt
     6181 ggcgtatgga gcgcctggcg tctggttaaa acgaccctca agcagcaaca gcttcgcggt
     6241 taacttccct ctggccggag caattccggc cttatccctc aaattttttg aagatttttg
     6301 acagttttcc ttgctaacaa tcatcattca ccacgtttat gattctctcc atcgacagca
     6361 acgacgctaa taccgcgccc ttgcacaaaa aaacaatcag cagcctgagt ggcgcagtgg
     6421 agagtatgat gaaaaaatta gaagatgttg gtgtactggt agcgcgcatt ttaatgccga
     6481 ttctgtttat taccgcaggc tggggaaaaa tcaccgctta ctctggtacc caacaatata
     6541 tggaagcaat gggtgtcccg ggttttatgc tgccgctggt gattctgctt gagttcggtg
     6601 gtggtctggc aatcctgttc ggtttcctga ctcgcaccac agccctgttt actgcgggct
     6661 ttacgctgct gacggcattt ttatttcaca gcaactttgc tgaaggcgtc aactcgctga
     6721 tgttcatgaa aaacctgaca atttctggcg gattcctgct gctggcaatt accggtccgg
     6781 gcgcgtatag catcgaccgc ctgctgaata aaaagtggta agcacgcgct atacttaacg
     6841 ataaaaaacg aggaggaagc tcctcgtttt tgctattgga ggagagaaaa atgggtcaac
     6901 tgattgacgg cgtctggcat gacacctggt acgataccaa atctaccggc ggtaaatttc
     6961 aacgttcagc ttccgcattt cgtaactggc tcactgccga tggcgcacct ggccccactg
     7021 gcacaggcgg ttttatcgca gagaaagatc gttatcatct ctatatttca ctcgcctgcc
     7081 cgtgggcgca ccgcacgctg atcatgcgca aactcaaagg actggaaccg tttatttccg
     7141 tttccgtagt gaacccgctg atgctggaaa acggctggac ctttgatgac agttttccgg
     7201 gagcaaccgg agatacgctc tatcaacatg aatttctgta tcagctttat ctccacgccg
     7261 atccacacta cagcggacga gttactgttc ccgtactgtg ggacaaaaag aaccacacca
     7321 tcgtcagcaa cgaatcaaca gaaatcatcc gcatgttcaa taccgcgttt gatgcgctgg
     7381 gcgcgaaagc gggtgattac tacccaccag ccctgcaaac gaaaattgac gaacttaacg
     7441 gctggattta tgacaccgtt aacaacggcg tgtataaagc tggttttgcc accagccagc
     7501 aagcctacga cgaggcggtg gcgaaagtgt ttgaatcgct ggcgcgactg gagcagattt
     7561 taggccagca tcgttacctg accggcaacc agctaaccga agccgatatt cgcctgtgga
     7621 ctacgctggt gcgttttgat ccagtgtatg tgacccactt caagtgtgat aagcaccgca
     7681 tcagcgatta cctgaatctg tatggcttcc tgcgcgatat ctaccagatg ccggggatcg
     7741 ccgaaactgt caatttcgat cacatccgta atcattactt ccgcagccat aagaccatca
     7801 accctacggg gattatttca attggtccgt ggcaggatct cgatgaaccg catggacgag
     7861 atgttcgctt cggttaaaaa aaggcacctt acgaggtgcc tctgataatt agctaagtgc
     7921 atgttgttca tatcggatgc ggcgtgaacg ccttatcaaa cctacaaaac atttatattc
     7981 aatatgttgc aatgaccgcg caggccagat aagcgtagcg catctggcga ttttgcgctt
     8041 gtcatcagtc ttagcgcgcc ttcacagcac tttattaatt ccttgaacga atatttactg
     8101 ccatttcatt cacgtctatt cttaatttgc tgctttaaaa acaagtgatt gagcaaaatt
     8161 gaggcaaaaa tggactggta tctgaaagta ataaaaaatt atgtcggttt ccggggtcgt
     8221 gcgcggcgca aagagtactg gatgtttatt ctggtcaaca tcatctttac gttcgtgctg
     8281 gggctgctgg ataaaatgtt aggctggcaa cgcgctggtg gcgaaggcat cctgacgaca
     8341 atctacggta ttctggtgtt tttaccgtgg tgggcagttc agttccgccg cctgcacgac
     8401 accgaccgct cggcgtggtg ggcactgcta ttcttaatcc cgtttatcgg ctggcttatt
     8461 atcatcgtct ttaactgcca ggcgggtacg ccaggcgaaa accgctttgg acctgatcca
     8521 aaactggaac cttaaaacaa atctgctcgc cggatgtctg actcatccgg cttttatcat
     8581 catgttgatg aatgaataat ttactaatgt ttatttaaaa tatttcaatg tcgttatata
     8641 aattacctat aaaaaataac catgtattta tcttaaatat caaaaccttt ataacatccg
     8701 aaaaagaaag atacgaatag catttacaca atacccggaa tagactttcg tcacttttaa
     8761 ttaaagggat gtttttatgc agtggtattt atccgtatta aaaaattatg ttggtttctc
     8821 tggtcgcgca cgtcgtaaag agtactggat gtttactctg attaacgcca tcgtcggcgc
     8881 tattatcaat gtcattcaat tgattttagg tctggagctt ccatatctgt ctatgcttta
     8941 tttgctggca accttccttc ccgtccttgc gcttgctatc cgtcgcttac acgataccga
     9001 ccgttcaggt gcctgggcct tgctcttttt tgttccgttt attggctggc tcgtgcttct
     9061 ggtttttttc tgcacagaag gtacttctgg cagcaatcgt tacggaaacg atccgaagtt
     9121 tggttcaaat taatcttaga attggggcga tatttcgccc ctttttatta acaataataa
     9181 atattatttt ccgctaaaaa gtttgggaat ttcccgcaga caccaggatt tcgcttcccc
     9241 catactgtca cgacgccagg ccataataat atcgatctca ctggtcgatt ccgggctgac
     9301 gacacgcaac cgcccttccg caatatcttt ttcgaccatc ggatacggca tcgtcgccac
     9361 gccaagcccc gccagtaatg cctgacgttt atcttcaatc gtgctcaccg ttaagcgcgg
     9421 ctgtttgtcc agcagttgta cggtcaacac cgggcgctca cgagcggtat ccgctaccgc
     9481 aattccacga tatttcacgc gcgtcacttc agataacggt tccggctcct gatgaatcgg
     9541 gtgatcaggc gcggcaacgt agacgttcat taacgtatag agcttgcgcg agttgatctc
     9601 cgacgaggaa cgaaaatgca tatccggcgc gataacaata tccgcccgcc cctgctccag
     9661 tcgttcctac gcccccgcca gcacttcggt gatgattgcc agttgggtat tggcttttgc
     9721 cgccagtttg tcgattaacg ggaaaaaggc aggtgtcggt accagcgctt cggtcacaat
     9781 ggtgagatgc gtttcccaac cgcgcgcgag ggcttccgca tcggtagtca gtttatctgc
     9841 ggcttccagc aaaacgcgcc ctcgctccag caacatcctc ccgacattgg tgaatttggt
     9901 gcgatggccc gagcggtcaa acagcaccac atccagctct tcttccagtt tttgcatggt
     9961 gtagctaagt gcggaaggca cgcgtcccag ctcatccgcc gccgccgcaa aactgccccg
    10021 gcgatcgatc gcatccataa cccgtagtgc ttccagcgtt aatgcccttt ctttggccat
    10081 ttcgttctca ttcaggaaat ttgaacatac cgggcagaat atctggctaa caatgtagcg
    10141 tccagcccct taccataaaa ggaagtaaag agaggtcaag aattatgatt actacccgaa
    10201 ctgccaggca gtgtggacaa gcagactacg gatggttgca ggcccggtat actttttcct
    10261 ttggacacta cttcgacccg aaattgttag gctatgcctc cctgcgtgtg cttaaccagg
    10321 aagtgctggc cccaggtgcc gcctttcagc cgcgaaccta tcccaaagtc gatattttaa
    10381 atgtgattct ggatggagaa gcagagtatc gcgacagcga aggcaatcat gttcaggcca
    10441 gcgccggtga ggcgttgctg ctctctaccc agccgggtgt cagctacagc gaacacaatc
    10501 tcagcaaaga caaaccgtta acacgaatgc agctttggct ggacgcctgc ccgcagcgag
    10561 agaatccgct gattcaaaaa ctggcgctta atatgggcaa gcagcaatta atcgcctcgc
    10621 cagagggtac gatgggaagc ctgcaattac gccagcaagt gtggctgcac catatcgtgc
    10681 tcgacaaagg cgaaagtgcg aatttccagt tgcatgggcc acgcgcgtat ttgcaatcga
    10741 ttcacgggaa atttcatgcg cttacgcatc atgaagagaa agcggcgctg acctgcggtg
    10801 atggggcgtt tattcgtgac gaggctaaca ttacgctggt tgccgattcc ccactgcgcg
    10861 ctttgctgat agatttgcct gtctagttgt ttttacaggg agatgatgat gagtaaaaaa
    10921 tcggccaaaa agcgccagcc agtgaagccc gtggtggcga aagaacctgc tcgcaccgcc
    10981 aaaaattttg gctatgaaga gatgttgagc gagctggaag ccatcgtcgc ggatgctgaa
    11041 acgcgtttag ccgaggatga agctaccgcg taaagacagg ttgccggatg cggcgaaaac
    11101 gccttatccg gcctacaaaa tccaacaaca ttaacgaatt aaacaacccg taggccggat
    11161 aaggtgcgcc agcatcgcat ccggcacaat actcaaaacc tggcgtttat ctggccttgc
    11221 tcgccataat ctcgataatc tgccgatccg tttgctgcat cgaatggctt gctaacgcac
    11281 acaggttggc aatcgactgc tcaacatcat gcgccacgat cccttcattg ccggtcacgg
    11341 cggtatcatc cagcgccatt aacaccgctt tccacgcagc cgaagcactg gtcgaaacct
    11401 tcatcgcgca gctgttcgac gcaccatcgc aaatcatgcc gctgacatcg ccgatcatac
    11461 tgctgatcgc catcgagata gtttcataac gcccatccac cagccatgcc atcccggcgg
    11521 cggcccccat tgctgcggtc gttgcggcac aaagtgcaga caaacgcggt aactggttat
    11581 ggatgtaaat tgcgctcaaa tgagaaagca tcagcgcacg cgccagtcgt tcatcatccg
    11641 ctccgaagtg ttctgctaca accaccacag gcattgttgc ggtgatcccc tggttacccg
    11701 agccggagtt actcatagcc ggaagcgtag cgccgcccat acgcgcatcg gatgccgcgc
    11761 tggtacgaat cacaatggat gaagagagat ctttcgccag caaaccgcgc tcgcactgtt
    11821 tttccagcgt cgcgccaata tgcagccccc acttaccgct caaaccttcc tgcgataacg
    11881 cacaatttag cttcgcggaa tcgagaataa agcggatcgc cgcaaacggg acttcattga
    11941 cgaacttcag gatctcagcc agcgtcgttc tggaaagcac cgtcagcgga gactcttgct
    12001 cgccctctgc cacacacgcc tgctgggtaa acaccacacc attgtgcgtc tcaatatgca
    12061 caatgttggt atgcccgccg acgatggtga cacacgccca cttctcaccg ttccagactt
    12121 tggcgcgtga gaagaggatt tcattgcaag gttcctggat cttaacggag actttccccg
    12181 ccgccagcag tgctttggca tcggcaattg cctgcgcagt tgcgtctttc agcacttcca
    12241 gcccggcgtt ggcatttcca cctaacgccc ccagcgccgc cgcaatcggc agccccacca
    12301 ttcccgtgcc gggaacggtg acgcccagac cgttcttcat cagatttggc gaaacccagg
    12361 cttctacacg ttcaaccgga ccttccagtt ctgccgcagc aaccgccgcc gccagcgcca
    12421 gtgaaatcgg ttcagtacat cccagcgccg gttttacttc ctcctgaacg gcgaggatgt
    12481 aacgctgcca taacggattt aaagtcgaat caaacatatt aaaaacctta aaatttcagg
    12541 taaatcaaga aaatgccagg aacggagaaa cacaaagcaa caaaccagtg acgataatca
    12601 ggtacagaga catccctttg tatttgtgca atgccggtac tttgtaaacc agccacgccg
    12661 ggatcaggca ccctaccatg ccgaaaatcg ggctacagat agaggtgaag ctcaacaccg
    12721 gtgcgttcag tacgatagcg ctccaggcca gcaaaatggc gaaaatcatg atgccgcgct
    12781 gaacaagatt ttcgttaatc ttctcggcag gcatcttgcg acgcaggatg ttcattacga
    12841 tcccttgcgt tgcttcgcga aagcctaaat agacgccaaa gaatgcagtc atgacagcaa
    12901 agatattgag aatgacgctg accactttca cccacgctgc gccgtcaccg ctaataaact
    12961 gtgcggcaat cgccagcgcg gaaatattct gctcgtaggc ttttaccgct tcgtcatgcc
    13021 ccatcgccag cgtgaacgac acggcgtaga aaaagacggt gacaaacaaa atgccaaacg
    13081 cgatattcat tgcccgcaat gctttatgcc gcgccacttc aatagatttt tcccgcgagc
    13141 gataagagat caccatcggg cttaacgtct ggataaacaa aatcgacgtc agggtaaacg
    13201 gcagcgtaat aatggcgttt ttcaccagca gccccagcgg cggtagcgaa ccgacgttat
    13261 acagatgcca cattcccacc atcgacacgc ccagcgccgc gaccaccagc agcttggtca
    13321 gcaccatgcc ggtcgaaatt ttgaataaca atttctcgcc gcgtgaagag atcgccacca
    13381 gaatgcaaat cagtaccaga ccataaaagg gactgtctga cagcaaccct tccgtcacgc
    13441 cgaaggtatg cagataggaa gcactatcgt tggtgatggc ggtggaatag acgaacatcc
    13501 aaatcaccag cattacgaaa tagagcgcac ctaacaggat gccccagttt ttacctaaat
    13561 aaccgctaat gacactcggg taatctttac attctggtga ttctgccagc gtattaataa
    13621 acaaccgctg aaacagatac attgccgggt aaccaatcac cgatgagagc aaaaataccc
    13681 acaatcccat caaaccgacc tgcaccggga gaaaaacaat ccccgcgcca atcgccatcc
    13741 cgatactcat aatcacccag ccggtgtcag tactgtcgaa tttgatcgct tctcgccact
    13801 cgctctcact cattccagca cgacccgccg gggtcgaagc gtctgcaatg acgcctttat
    13861 tcgatgcaat ttccataatt tctcgctcaa tattttgtag ggcttattat ttttttccga
    13921 gccgcatcaa ggcgatatgc ggtctacgtt tttgcaggcg agttattaga ataaagaaat
    13981 gatacgcgcg aggtcagaga aaatcttcac aatcaagact ttgaaataac ggagatggat
    14041 aagaattttc tactaaatta atcgcaagag aatattcatt ctctatttgc ggcagatcac
    14101 aaaaaaaagg tgcacatttg tgcacccaag gatgaaagct gacagcaatg tcagccgcag
    14161 accactttaa tggccagtcc tccgcgtgat gtttcgcggt atttatcgtt catatctttg
    14221 ccggtttcat acatcgtctc gatcacttta tcgagtgaaa cacgcggtgc cgaggtgcgg
    14281 cgcatcgcca tccacgcggc gtttactgct ttcacggcat taatggcatt acgttcaatg
    14341 cacgggattt gtacctgtcc ggcaaccgga tcgcaggtca gcccaaggtt atgctccatc
    14401 gcgatttccg ccgcattgca tacctgcgcc ggactgccgc ccagtagttc agttaaccct
    14461 gccgccgcca ttgaacaggc cacgccaatc tccccctgac agccgacctc cgcgccagag
    14521 atggaggcgt tcattttata cagcgcgcca atagcccccg ctgccagaaa atagcgggca
    14581 attgaccgct cgtttaccgg acgacggaac ttatcgtaat aagccagcac tgccggaata
    14641 atgccgcacg caccgttagt cggtgccgtt accacgcgcc cgccagctgc gttttcttca
    14701 ctaaccgcca gcgcgtacat gttgatccag tcgatgacat tcatcggatc gttagagatg
    14761 ttatcgctag aaaccagctg acgacgcagc gctacggcac gacgcggcac attgagcgga
    14821 ccaggcagca cgccttcagt gttcatcccg cgttcaatac cgtcatgcat cacttgccag
    14881 atacgggcaa atcctgcgtc aatttccgct ttgctgcgca gcgccagctc gttgtgcatc
    14941 atcaggccag atatagacag gccgttgtaa tcacacattt tcagcagttc acctgctgag
    15001 tggaaatcgt aaggtacgga cgtttcgaca tcgtgcgaca ggccgaagtg ttcttcttcg
    15061 acaataaacc cgccgccgac agagtaatag gttttactta atagcgcttc ctgtcccttc
    15121 caggcagtga tccgcattcc gttctcatgg cgaggcaaca tttcgggatg gaagataatg
    15181 ttctttgcta caggaaaatc aacaatatgc gcaccagatg ccactggcag ccgcccgctg
    15241 cgcgttacta actctataaa tgcagggatc tcatcaatga caacatcctg cggactgttt
    15301 cctgccagtc ccatgataat ggcgacatcc gtggcatggc ctttgcccgt cagtgacaac
    15361 gacccataca gatcgaccac aatatggctc gtcgcggtta ataagccgct actttccagc
    15421 cgatcaataa aactttttcc ggcattcatt ggccccacgg tatgcgaact ggagggacca
    15481 atcccaattt tgaaaatatc gaatgcacta atcatatcca caccctcgga ttgccgttca
    15541 gtgaagtgga gcggaacgac cttgcgaccg tcccgctcac gaggctttac gcactacgta
    15601 ctgcgatagc ttcaatttcc agcttcacat ctttcggcaa acgcgcaacc tgcacacagc
    15661 tccgggtcgg gtaggtcgcc tgatgctcat cgaagaactg cttatagact tcattgatgg
    15721 tggcaaaatc attcagatcg gtgatgaaca cggtcatctt gatgatatcg cccacgctca
    15781 gcccggcagc aaccacgatc gctttgacgt tttcgaggct taaacgcgcc tgatcttgca
    15841 catcagccgg gatctcaccg gtctgcgggc aaaccggaat ttgcccggag gtgaagacca
    15901 tgctgcctaa atcaacgccc tgaacataag ggccgattgc gcctggggca cgttgcgttt
    15961 cgataatctt tttcatacat cctccggcgt cagagcgcct gggtaaaagt acgtgaaata
    16021 acatcctgtt gctgttcacg ggtcagtgcg ttgaagcgca cggcgtagcc agagacacgg
    16081 attgtcaggt taggatattt ttccgggtgc tcgatggcat ccagcagcat ttcccgattc
    16141 attacgttga cgttgaggtg ttgaccgcct tcgacatccg cttcgtggtg gaaataccca
    16201 tccagcaggc cgacaaggtt ggttttacgt actggatctt ctttgcccag cgccgcagga
    16261 acgattgaga aggtgtacga gatcccatct ttggcgtagg tgaacggcag tttcgccacc
    16321 gacgtcaatg aggccacggc acctttgcgg tcacgaccat gcatcgggtt agcgcccggc
    16381 gcgaacggtg ttccggcgcg acgaccgtcc ggcgtattac cggttttctg gccgtacacc
    16441 acgttagaag tgatagtcag aatcgactgg gtagggacgg cgttgcgata ggttggcagc
    16501 gctttaattt tcttcataaa gcgttcaacc aggtcgcagg caatgctgtc tacgcgctcg
    16561 tcgttgttgc cgtactgcgg atattcaccg tcgatttcaa agtccaccgc caggccgttt
    16621 tcgtcacgga ttggtttcac gcgggcatat ttgatggcag acagggagtc cgtcgccacc
    16681 gacaggcccg cgatgccgca tgccatagtg cgatagacat cacgatcgtg cagcgccatc
    16741 agcgaagctt cgtagctgta cttgtcgtgc atgtagtgaa tgatattcag cgcgctgatg
    16801 tactgcaccg ccagccagtc catgaagtga tcgaggctgt ccatcacttt gtcgtaatcc
    16861 agcacgtcgt ccatcagcgg tgctgttttc ggcccgacct gaatcttcag cttctcgtcc
    16921 accccgccgt taattgcgta cagcagtgtt ttcgccaggt tagcgcgtgc accaaagaac
    16981 tgcatttgct taccaatcac cattgggctg acgcaacaag caattgcgta atcgtcgctg
    17041 ttgaagtcag tacgcatcag atcgtcgttt tcatactgca aggaagaggt gacgatcgac
    17101 acctgcgcgg catatttttt gaaggcaatc ggtaattctt ccgaccaaag aatggtcagg
    17161 ttaggttccg gtgccggccc catagtgtgc agggtgtgca aatagcggaa ggagtttttg
    17221 gtcaccagcg tacgaccgtc cagccccatc ccgccgatca cttccgtcgc ccagattggg
    17281 tcgccggaga acagcgaatc aaattccggc gtacgcaaga agcgcaccat acggatcttc
    17341 ataatgaagt gatcgatcag ttcctgtgcc tgctgctcat tgagtacgcc tgctttaaag
    17401 tcgcgctcaa tgtagatatc gaggaacgac gccgtgcgcc ccagcgacat cgcgccgccg
    17461 ttctgtgatt tcactgccgc cagataagcg aagtagagcc actgcaccgc ttcctgcgca
    17521 ttctgcgccg ggcgagagat atcaaagcca tatttcgccg ccatttcctg aatctgcaac
    17581 agcgcatgac gatgctctgc cagctcctca cgcagacgga tggtggcttc cagatcttcg
    17641 cctttttcca gacgggactg gagatcggca aattgcagtt cgcgttcacg caccagataa
    17701 ctgatgccat acagcgctac gcggcgatag tcaccgataa tgcgcccacg gccatagcca
    17761 tctggtaaac cggtcagcac gccagattta cggcagcgca gcatatccgg tgagtaaaca
    17821 tcaaatacgc cctggttatg ggttttacgc agatcggtaa acagatattc aaattcactg
    17881 tccatttcac ggccataggc gtggaatgaa cttttaatca tattaatgcc accgaacgga
    17941 tgcagcgcgc gctttaatgg cgcatccgtt tgcagaccaa caattttttc cagcggctgg
    18001 ttaatatatc ccgcatcatg agcggtaatt gtggtggcaa tattggtatc gaaatcaacc
    18061 ggcgcgtggg ttgcgttttc gatacggatg ccttccatta ctttttccca caattccgtg
    18121 gtggcaggcg tcgcttcggc gaggaaagat tcatcgcctt catacggtgt atagttatgt
    18181 tgaataaaat cgcggacatt aatttcgttt ttccagtccg tacctttaaa gccaagccat
    18241 gcgtcggcgt acagcttatc gctggtatca atatctatct tcatgaaaaa taatctctct
    18301 acaatacttc aactaaatta tgcaaattct gcgggcgcgt taactttgcc taaatgaatg
    18361 gcatccaaag caatcatttt ttcttcgtta gtcggaataa cggcacaaat gacacgcgca
    18421 ttttcactgg aaacaattcg ctctccaaaa gagttagagc gattattcat ttctgtatca
    18481 atcactacgc ctaatacagc caaatgttcc atgaccagac gacgaattaa acttgaattc
    18541 tctcctattc cgccggtgaa tataattcca tccaggcgac gtaatgaagc tgcgtgtccg
    18601 gcaatatgac gggcaattcg gtgaacaaag gttttaattg ccagttgcgc gcgttcgtga
    18661 ccttcatgcc aggctttttc cagaacacgt aaatccgaag aaagaccgga aatacctaat
    18721 aatcccgact ctttattcac tacgcgttcc aggtcaccca ggctctggtt ggtttggctg
    18781 gcgacccagg acatcgcacc aaagtcgaca tcgccactgc gggttcccat catcaagcct
    18841 tccagcggcg tcattcccat tgaagtatca acactctgac cgttgcgaac cgcgcagatt
    18901 gacgcgccat tgccaagatg cgccacaacc aggccggagt catcttccgc cagattcagc
    18961 agagaatgtg cgcgctggga aacatagcgg tgcgacgtgc cgtggaaacc ataacggcgt
    19021 acacctaact cttcataata tttccacggc aggccgtata aataagcttc cggagccatc
    19081 gtctggtgga aactggtatc aaataccgcc acctgagtta cgcccggaaa taattgctgc
    19141 gccgattcaa taccacttaa attggcgtaa ttatgcaggg gtgccagtgg agaaacgcga
    19201 cggatattat caatgacttc atcggtaata atggcggact cggtaaaaat actgccgccg
    19261 tgagcgatgc ggtggccaat taaggccaca ctgtcattta aattccgttt ttccagttca
    19321 aatgcaattg ccttcaatgc accttcgtag ctgtggtgag ccagcggtgc tggctctccc
    19381 ccatttacgg ataagaatgc attttccgag ttaataccgt cggcaatacc tgacattaat
    19441 acttcacagt cgctggcatc gagcacggaa aacttaatcg aagacgaacc acagttaata
    19501 accaaaacaa ccggaaattc attcatctct tttctcatcc tgagttacgg attaaaacag
    19561 tttgtatacg atgttcagga tggtcagcag accaatcacg gtaacaaaca cgttatccag
    19621 acgaccacgg tatttcgcca gagacggcgc tttacggatg gcatacatcg gcaacaggca
    19681 cagcagggat gcgataatcg gtgcgcccat ggcttcaatc aggtcgagga tgttcgggtt
    19741 ggcgtaggca acaacccagg tggagcccat gatgaagatc atgctgatag tgttcagttt
    19801 acccagcgac actttagttt tgtcgccttt ataaccaaac ttcaggacca ggccattcag
    19861 accttccagc gttcccagat agtgaccgaa gaaagatttg aagatagcca cgagtgcgat
    19921 gatggaagcc gcatattcca gtgtaatcgc gaacgttgtt ttggtaccgg tcatggacgc
    19981 aaagtggtta gccagataag aaagcactgg aatattctgc gctttggctt ccgccatgtt
    20041 ggccggagac agcgtaaata ggcagctaaa ggcaaagaac atcaccactg caaccatcag
    20101 catgctggca cgagaaatga tttgggaaca tttacgttcg gtgaagtcgc gaccgaagtc
    20161 tttctcatac tcttcacgtt tagaaaccac gaaggaagag acgattggcg agaaattaaa
    20221 ggagaaaacc atgatggaaa tccccagcca gacggtgatc aggataccgt catgaccggt
    20281 taacgacagc gaaccgaggt caacctggtc gataactgca gagttccagt aagggatcag
    20341 cgacagagaa atcagcacca ggctggcgat aaacggccat accaggtagc tcatcacttt
    20401 aaccatcaga tccttaccaa accagatgac gaaagccatc agcagcagca ggaacagcgc
    20461 cacaaagccg cgattcagcg gtgcaaagcc gagctggttt tcccagaacg tcataaaggt
    20521 attggtaata gtaacgccat aaatccacag cagtgggcaa atcgcgaaga agtacaggaa
    20581 cgtgataacc acgccgccag ttttaccaaa atgctcttcc accgtttccg taatgttgcc
    20641 ggaagggtta gagccagaaa gacatagacg cgccagcgcc cggtggcaat aaaatgcgat
    20701 ggggtatgcc aataccaaca tcagaagaat cgggatcagt ccgccaaaac ctgcgcggat
    20761 agggaagaac agcaccccgg cgccgattgc cgtaccaaac aagcctaacg tccatgtggt
    20821 atctgattta cgccaggacg attgttttgt ctggctggat acaatgctat ctgaagtact
    20881 catatcctat cctcaacgaa ttaattaagc gtcaacgaaa ccggtgattt gagagacgcg
    20941 agaaagatcg atattgccgc cggaaataat actgacggtt tttctgtttt gaatatattg
    21001 gtctaattta ccgcttaata atgcagcaca tgccagagcg cctgcgcctt cggtgacgac
    21061 tttattgcgc tgaattaagg caatcatact gtttctgatt tcgtcttcgc tgaccagcac
    21121 gatgtcatcg actaattcac gaacgatttc gtaagttaaa ttacccgggc gggagacatc
    21181 acaaccatcc gccagggtgc cggtagttcg gtgcgtggtt atttctccgg agtggaaaga
    21241 agccgccatg ccgtgaacgt tttcagactg tacgccaata acacgaatgg tcgggttaat
    21301 agatttaatt gccaccgcaa taccagcaat taaaccgcca ccaccaattg gcacaatcac
    21361 gttatcgaca tcatagagat cttccataat ttccagacca atcgttccct ggccagcaat
    21421 cactttcgga tcatcgtaag gtgggataaa aatacggcct tccatttcga caatttcgct
    21481 cactttagcg atagtgtcgt tgaagttatc accatgcaga acgacttctg cggagtagtc
    21541 gcacgttgcc gctactttgg attttggcgc accttttggc atcaccactt taccgtcgat
    21601 acccagcatc gcgcaggaga gcgaaacccc ttgcgcatgg ttgcccgcag aacaggccac
    21661 cacgcctttg cgtttttccg catcggtcag tgaacttaat ttattaaatg cgccacgaat
    21721 tttaaatgaa cccgtacgct gcatattttc aaacttcagg aatatttcac ctttgcaacg
    21781 ttcactaaaa tagttggagc gaggcatgcc tgttttataa attcgcccag ccagtcgttg
    21841 tttcgcttca ataatatcat caatagcaat cggcagatcg tatgtaatat gcattataaa
    21901 acctcttcgc ctgaattaaa tatgtaggta accgtaaccg acacctgcaa aacagacagg
    21961 tggattattt atgtttaaat aaaaacaaat aactaaccaa cttctattaa ttgccttcgt
    22021 ctacacccat tataagatga atactctttg gctaattcca ccaaaaccga tgctgctttt
    22081 ttaatacgat aatttttcga ccataccgcg gcatattgtg ccacaggtaa tgtttcttca
    22141 accggaatag taataaattg attagaacca aaaggtgacg tcatatcaca aggaattaca
    22201 gttaagaaat cagcattgag aacaagatta taaattgtca cgactgagtc ggttttaacg
    22261 atgttttcaa tactgatgcc atttctttgt aacgtagtaa gcagttcgct gtagtacccc
    22321 atattagttt gtggcaacac ccactgttcg ttcttcaacg actccagcgt ggtggtgccg
    22381 gtgcatgttc gggacttact ggctaccagc acaaactcgg actcgaacag cggctcaaca
    22441 tgtaaatcct gaagcttcat ttctgcactt aacgtaccaa tcgcaaaatc cagtcgaccg
    22501 tcgcggattg ccggtaagaa cgaagacagt tgcgcttcat acatagaaac ctgcgctttc
    22561 gggaacacct ctttgaactt gttgatcatc cctgacataa aagtaaaacc aatcaatgaa
    22621 ggaaaaccaa atgagacttc caccaccgcc tcagaagaca taccgcttat ctcattaacc
    22681 atatttttca tttcacgggt aatggattcg gaacgggaga gtaacaattg accggcaggt
    22741 gttaatgtta caccggtatt tttccgcacc actaattcca caccaaaata atcttcaata
    22801 tcgttaatga ttttactgac ggccggttga gttaacccta attcttttgc agccgagccg
    22861 atagagccac ttctaatgac ttcctgaaag actaccaggt gctgcgtttt cggaagaaga
    22921 atagtgctca taacgaccta cgttaattac ctcattgacg gcatgaagtg tatcaaaatg
    22981 aaatgaacag gatatgtgcg accactcaca aattaacttt caatactttc cagggtatcg
    23041 tcattacaat taaatacctt taaatatcaa caagttaaag cataaaaatc agcataaacc
    23101 ctgatttttg tcaaaaaaaa tgacggggat aaccatattt tatggcgata acatcattcg
    23161 ttatgtggtt aatacaaaaa aggggctgag accatatttc aagcaattac cggataatta
    23221 ccaggcaaaa tttgagttaa atttaatgag caagttctca aattttatta taaataaacc
    23281 attttcagcg ataaatactg cggcacgtca cattttttca cgctatttgt tggagaacaa
    23341 acatttattt tatcaatatt ttaaaatttc gaatacaggt attgatcatc tcgaacaatt
    23401 gattaacgtc aactttttct cttctgacag gacgtcattt tgtgaatgca atcgttttcc
    23461 ataaattctt ctcccctcat aggcgacgaa tagcattttg tgttgaggat cacaaaacga
    23521 ataattgctg atcgccgcga taaggtcaga caaagacaac aagggaaatt ttcacagagc
    23581 ttttgatcgt cgtaggccac agaatgttgc ttcgtttact gtaacgccgg gtaaatgagc
    23641 gttttttgat agtgcgaaag aaccctgcga ggaaaataag catctattat tgtttgtcat
    23701 gacgacaagg caggcatcaa tgaaaggctt cccaattgca catatttttc acccttcaat
    23761 cccgccaatg cacgcagtgg ttaacaatca caatagaaat attgattatt ggacggtaaa
    23821 aagaaagttt gcaga
//
LOCUS       DAKISA010000065        23656 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.065, whole genome shotgun sequence.
ACCESSION   DAKISA010000065 DAKISA010000000
VERSION     DAKISA010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23656
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.065"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(383..1360)
                     /gene="epmA"
                     /locus_tag="OVW45_002629"
     CDS             complement(383..1360)
                     /gene="epmA"
                     /locus_tag="OVW45_002629"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCV5527041.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1685..3493
                     /gene="frdA"
                     /locus_tag="OVW45_002630"
     CDS             1685..3493
                     /gene="frdA"
                     /locus_tag="OVW45_002630"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCV5527042.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3486..4220
                     /gene="frdB"
                     /locus_tag="OVW45_002631"
     CDS             3486..4220
                     /gene="frdB"
                     /locus_tag="OVW45_002631"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCV5527043.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4231..4626
                     /gene="frdC"
                     /locus_tag="OVW45_002632"
     CDS             4231..4626
                     /gene="frdC"
                     /locus_tag="OVW45_002632"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCV5527044.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4637..4996
                     /gene="frdD"
                     /locus_tag="OVW45_002633"
     CDS             4637..4996
                     /gene="frdD"
                     /locus_tag="OVW45_002633"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCV5527045.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5059..6192
                     /locus_tag="OVW45_002634"
     CDS             5059..6192
                     /locus_tag="OVW45_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCV5527046.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6281..6814
                     /gene="blc"
                     /locus_tag="OVW45_002635"
     CDS             6281..6814
                     /gene="blc"
                     /locus_tag="OVW45_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCV5527047.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6811..7128)
                     /gene="sugE"
                     /locus_tag="OVW45_002636"
     CDS             complement(6811..7128)
                     /gene="sugE"
                     /locus_tag="OVW45_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCV5527048.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7303..7449)
                     /gene="ecnB"
                     /locus_tag="OVW45_002637"
     CDS             complement(7303..7449)
                     /gene="ecnB"
                     /locus_tag="OVW45_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCV5527049.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7560..7685)
                     /gene="ecnA"
                     /locus_tag="OVW45_002638"
     CDS             complement(7560..7685)
                     /gene="ecnA"
                     /locus_tag="OVW45_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCV5527050.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7737..8303)
                     /gene="efp"
                     /locus_tag="OVW45_002639"
     CDS             complement(7737..8303)
                     /gene="efp"
                     /locus_tag="OVW45_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCV5527051.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8345..9373
                     /gene="epmB"
                     /locus_tag="OVW45_002640"
     CDS             8345..9373
                     /gene="epmB"
                     /locus_tag="OVW45_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCV5527052.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            9763..10632
                     /locus_tag="OVW45_002641"
     CDS             9763..10632
                     /locus_tag="OVW45_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCV5527053.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLNAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGTVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(10835..11188)
                     /locus_tag="OVW45_002642"
     CDS             complement(10835..11188)
                     /locus_tag="OVW45_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCV5527054.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(11326..12972)
                     /gene="groL"
                     /locus_tag="OVW45_002643"
     CDS             complement(11326..12972)
                     /gene="groL"
                     /locus_tag="OVW45_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCV5527055.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(13016..13309)
                     /locus_tag="OVW45_002644"
     CDS             complement(13016..13309)
                     /locus_tag="OVW45_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCV5527056.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            13585..14841
                     /gene="yjeH"
                     /locus_tag="OVW45_002645"
     CDS             13585..14841
                     /gene="yjeH"
                     /locus_tag="OVW45_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCV5527057.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LTCFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(14863..15333)
                     /gene="fxsA"
                     /locus_tag="OVW45_002646"
     CDS             complement(14863..15333)
                     /gene="fxsA"
                     /locus_tag="OVW45_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCV5527058.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDR"
     gene            15670..17106
                     /gene="aspA"
                     /locus_tag="OVW45_002647"
     CDS             15670..17106
                     /gene="aspA"
                     /locus_tag="OVW45_002647"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCV5527059.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            17224..18525
                     /gene="dcuA"
                     /locus_tag="OVW45_002648"
     CDS             17224..18525
                     /gene="dcuA"
                     /locus_tag="OVW45_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCV5527060.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            18641..18979
                     /gene="cutA"
                     /locus_tag="OVW45_002649"
     CDS             18641..18979
                     /gene="cutA"
                     /locus_tag="OVW45_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCV5527061.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            18955..20652
                     /gene="dsbD"
                     /locus_tag="OVW45_002650"
     CDS             18955..20652
                     /gene="dsbD"
                     /locus_tag="OVW45_002650"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCV5527062.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTHLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGMLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPIFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            20689..21264
                     /locus_tag="OVW45_002651"
     CDS             20689..21264
                     /locus_tag="OVW45_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCV5527063.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            21371..21446
                     /locus_tag="OVW45_002652"
     tRNA            21371..21446
                     /locus_tag="OVW45_002652"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21404..21406,aa:Phe,seq:gaa)
     gene            22063..23600
                     /gene="cadC"
                     /locus_tag="OVW45_002653"
                     /pseudo
     CDS             22063..23600
                     /gene="cadC"
                     /locus_tag="OVW45_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
BASE COUNT         5932 a         5997 c         5818 g         5909 t
ORIGIN      
        1 aaggcgaatg gaataaagaa taacaatgca gaaaatatat aaaagggaat cgcactataa
       61 cccattaagt aataagccga tgggctattg gcaaatccaa atacggaagt aaagatcatc
      121 aatatgagtc ctatcagact catctttttt atcgtgtgag gcatgaaacc atccttcatt
      181 tcagcgcgtg tataacacgt gcccactgaa ctgcactgcg caacattgag caggcagcgt
      241 aaaaaccaat acagaattga tacccggatg atatcaaata tccagtctta aattgtggcc
      301 tttaacggaa aaatgaaaac atatgctaca gaatggcgct tatcacgcca ttcttcgctg
      361 ttaattcagt aatttttcag aattatgccc ggtcaacgct aaaggcgatg acttcagcca
      421 gtgtctccgc gcccagcgcc aacatcacca gacgatcaac acctaatgcc acgccggaac
      481 agtcaggcat accgactttc aaggcttcaa tcagattctg gtcaatgggg tgctgcggca
      541 aaccgcgcgc cgcgcgctta cggttatctt gttcaaagcg ttgttgctgc tcacgggcat
      601 ccgttaattc atggaaacca ttcgccagct caataccttt ataataaacc tcaaagcgtt
      661 cagcgacccg atgatcttcg gtactgattt gcgccagtga tgcctggctg gctggaaagt
      721 ggtacacaaa ggtcggtttt tctttgccaa tatttggctc tacgccaaag gtaaacagca
      781 attgtagcaa cgtgtcgcgg tcttcttcgg tatcagcaac gttactcaaa tccagtttcg
      841 ctgcgacttc ccgcagttgc gttttgtcgg cagagagcgg gtcaatttcc agataacgca
      901 agaaagcttg ttgataagaa aggctttctg ctgccgggca gtccagcacc tgttgtaaga
      961 gatcgtccac ctcgttcatc aaccggtaca tatcatagtg cggtcgatac cactcaagca
     1021 tagtgaactc agggttgtga taacgcccca tctcttcatt acggaagctg cggcacagct
     1081 ggaataccgg cccacaaccg gcaaccagca ggcgtttcat atggtattcc gggctggtca
     1141 ttaaccagag attcatcccc tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga
     1201 ccaaatgaat atcggttacc gtcgcctggc tcatacaagg cgtctccacc tccagcactc
     1261 cacgatcggc aaagaaacga cggatctccg ccataatcgc cgcacgtttt aataagttag
     1321 gaatggatgc gctcggctgc caggatgccg tttcgctcat agttaaatct ccagtttttg
     1381 acaagggcac gaagtctact cgcaacgcga cggcgagaca aattttacgc aggaatcaaa
     1441 cagcggtggg cagtgactaa aaaaagcacg atctgatggt ttagtaatta aattaatcat
     1501 cttcagtgat aatttagccc tcttgcgcac taaaaaaatc gatctcgtca aatttcagac
     1561 ttatccatca gactatactg ttgtacctat aaaggagcag tggaatagcg ttcgcagacc
     1621 gtaactttca ggcacttacc ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa
     1681 tgtcgtgcaa acctttcaag ccgatcttgc cattgtaggc gccggtggcg cgggattacg
     1741 tgctgcaatt gctgccgcgc aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt
     1801 atacccgatg cgtagccata ccgttgctgc agaagggggc tccgccgctg tcgcgcagga
     1861 tcatgacagc ttcgaatatc actttcacga tacagtagcg ggtggcgact ggttgtgtga
     1921 gcaggatgtc gtggattatt tcgtccacca ctgcccaacc gaaatgaccc aactggaact
     1981 gtgggggtgc ccatggagcc gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg
     2041 catgaaaatc gagcgcacct ggttcgccgc cgataagacc ggcttccata tgctgcacac
     2101 gctgttccag acctctctgc aattcccgca gatccagcgt tttgacgaac atttcgtgct
     2161 ggatattctg gttgatgatg gtcatgttcg cggcctggta gcaatgaaca tgatggaagg
     2221 cacgctggtg cagatccgtg ctaacgcggt cgttatggct actggcggtg cgggtcgcgt
     2281 ttatcgttac aacaccaacg gcggcatcgt taccggtgac ggtatgggta tggcgctaag
     2341 ccacggcgtt ccgctgcgtg acatggaatt cgttcagtat cacccaaccg gtctgccagg
     2401 ttccggtatc ctgatgaccg aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa
     2461 tggctaccgt tatctgcaag attacggcat gggcccggaa actccgctgg gcgagccgaa
     2521 aaacaaatat atggaactgg gtccacgcga caaagtttct caggccttct ggcacgaatg
     2581 gcgtaaaggc aacaccatct ccacgccgcg tggcgatgtg gtttatctcg acttgcgtca
     2641 cctcggcgag aaaaaactgc atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta
     2701 cgttggcgtc gatccggtta aagaaccgat tccggtacgt ccgaccgcac actacaccat
     2761 gggcggtatc gaaaccgatc agaactgtga aacccgcatt aaaggtctgt tcgccgtggg
     2821 tgaatgttcc tctgttggtc tgcacggtgc aaaccgtctg ggctccaact ccctggcgga
     2881 actggtggtc ttcggccgtc tggccggtga acaagcgaca gagcgtgcag caactgccgg
     2941 taatggcaac gaagcggcaa ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga
     3001 tctggttaac caggatggcg gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc
     3061 aatggaagaa ggttgcggta tctaccgtac gccggaactg atgcagaaaa ccatcgacaa
     3121 gctggcagag ctgcaggaac gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt
     3181 caacaccgac ctgctctaca ccattgaact gggccacggt ctgaacgttg ctgaatgtat
     3241 ggcgcactcc gcaatggcac gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg
     3301 ttgcaccgag cgtgacgacg tcaacttcct caaacacacc ctcgccttcc gcgatgctga
     3361 tggcacgact cgcctagagt acagcgacgt gaagattact acgctgccgc cagctaaacg
     3421 cgtttacggt ggcgaagcgg atgcagccga taaggcggaa gcagccaata agaaggagaa
     3481 ggcgaatggc tgagatgaaa aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg
     3541 ataccgcacc gcatagcgca ttctatgaag tgccttatga cgcaactacc tcattactgg
     3601 atgcgctggg ctacatcaaa gacaacctgg caccggacct gagttaccgc tggtcctgcc
     3661 gtatggcgat ttgtggttcc tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta
     3721 aaaccttcct gcgtgattac accgacggta tgaaggttga agcgttagct aacttcccga
     3781 ttgaacgcga tctggtggtc gatatgaccc acttcatcga aagtctggaa gcgatcaaac
     3841 cgtacatcat cggcaactcc cgcaccgcgg atcagggtac taacatccag accccggcgc
     3901 agatggcaaa gtatcaccag ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt
     3961 gcccgcagtt tggcctgaac ccagagttca tcggtccggc tgccattacg ctggcgcatc
     4021 gttataacga agatagccgc gaccacggta agaaggagcg tatggcgcag ttgaacagcc
     4081 agaacggcgt atggagctgt actttcgtgg gctattgctc cgaagtctgc ccgaaacacg
     4141 tcgatccggc tgcggccatt cagcagggca aagtagaaag ttcgaaagac tttcttatcg
     4201 cgaccctgaa accacgctaa ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg
     4261 ccaatgacgt ccacctggtg gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa
     4321 ggcacggcgg ttccggctgt gtggttcagc attgaactga ttttcgggct gtttgccctg
     4381 aaaaatggcc cggaagcctg ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg
     4441 atcattaacc tgatcactct ggcggcagct ctgctgcaca ccaaaacctg gtttgaactg
     4501 gcaccgaaag cggccaatat cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc
     4561 aaaagtctct gggcggtaac tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac
     4621 tggtaaggag cctgagatga ttaatccaaa tccaaagcgt tctgacgaac cggtattctg
     4681 gggcctcttc ggggccggtg gtatgtggag cgcaattatt gcgccggtga tgatcctgct
     4741 ggtgggtatt ctgctgccac tggggctgtt tccgggtgat gcgctgagct acgagcgcgt
     4801 tctggcgttc gcgcagagct tcattggtcg cgtattcctg ttcctgatga tcgttctgcc
     4861 gctgtggtgt ggtttacacc gtatgcacca cgcgatgcac gatctgaaaa ttcacgtgcc
     4921 tgcgggcaaa tgggttttct acggtctggc tgctatcctg acagttgtca cgctgattgg
     4981 tatcgttaca atctaacgta tcgccaatgt aaatccggcc cgcctatggc gggccgtttt
     5041 gtatggaaac cagaccttat gttcaaaacg acgctctgcg ccttattaat taccgcctct
     5101 tgctccacat ttgctgcccc tcaacaaatc aacgatattg tgcatcgcac aattaccccg
     5161 cttatagagc aacaaaagat ccccggtatg gcggtggcgg taatttatca gggtaaacct
     5221 tattacttta cctggggcta tgcggacatc gccaaaaagc agcccgtcac acagcaaacg
     5281 ttgtttgagt taggttcggt cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt
     5341 gctcgagggg aaatcaagtt aagcgatccc gcaacaaaat actggcctga acttactgct
     5401 aaacagtgga atgggatcac actattacat cttgcgacct acaccgctgg cggcctgcca
     5461 ttgcaggtgc cggatgaagt gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg
     5521 cagcctgcat gggctccagg aacacaacgt ctgtatgcca actccagtat cggtttgttc
     5581 ggcgcactgg ctgtgaagcc gtctggtttg agttttgagc aggcgatgca aactcgtgtc
     5641 ttccagccac tcaaactcaa ccatacgtgg attaatgtac ctcccccaga agaaaagaat
     5701 tacgcctggg gatatcgcga aggtaaggca gtgcatgttt cgccaggggc gttagatgct
     5761 gaagcttatg gtgtgaagtc gaccattgaa gatatggccc gctgggtgca aagcaattta
     5821 accccccttg atatcaatga gaaaacgctt caacaaggga tacaactggc acaatctcgc
     5881 tactggcaaa ccggcgatat gtatcagggc ctgggctggg aaatgctgga ctggccggtc
     5941 aatcctgaca gcatcattaa cggcagtgac aataaaattg cactggcagc acgcccggta
     6001 aaagcgatta agcccccaac tcctgcagta cgcgcatcat gggtacataa aacgggggcg
     6061 accggcggat ttggtagcta tgtcgcgttt attccagaaa aagagctggg tatcgtgatg
     6121 ctggctaaca aaaactatcc caatccagcg agagtcgccg ccgcctgtca gattctcaac
     6181 gctctacagt aaaatttcat cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc
     6241 tgtcatctac acttagaaaa aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt
     6301 tgccgcagcg acagctgcat ttctggtcgt tgcctgcagt tctcctacgc cgccgcgtgg
     6361 cgtgacagta gtgaataatt tcgacgctaa acgctatctc ggtacctggt atgagattgc
     6421 ccgttttgat caccgttttg aacgtggact ggaaaaagtc accgcaacat acagtctgcg
     6481 tgatgacggc ggcctgaatg tcatcaataa aggctataac cctgacagag aaatgtggca
     6541 gcagagtgaa gggaaagcgt actttaccgg cgcaccaaca cgcgctgcgc tgaaagtgtc
     6601 attctttggt cctttctatg gcggttataa cgtcattgca cttgatcggg aataccgcca
     6661 tgcgctggtt tgcggcccgg accgcgacta cctgtggata ctctcccgca caccaaccat
     6721 ttctgacgaa gtgaaacagg agatgctggc agtcgcgacc cgggaagggt ttgatgtcag
     6781 taagtttatt tgggtacagc agcccggtag ttagtgagtg ctgagtttca gaccaataat
     6841 ccccaatacg attaacgcca gactcgccag gcgcatcggg ttagcggact caccgagcag
     6901 cacaatgccg gtgatggccg cgccgactgc gccaataccc gtccacacgg cataagccgt
     6961 ccctaccggt aacgatttca tcgcccaggc aagtagcgcc atactgacta tcatcgccgt
     7021 cacagtaata acacttggcg tcaaacgact aaagccgtgg gtatatttca ggccaacggc
     7081 ccataccact tccagcagac cagcaataac taagataatc caggacatat caggctccgg
     7141 agaattgggg ccgtccccgg tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg
     7201 ttgaacggat attttgccag gtggcagggg aatttcaact attacctttg tgactatcgc
     7261 aaaaaaacaa acggcacgac ataactgtcg taccgttatt gcttattgct gcgctttcgt
     7321 tgctgcacca gaaatcgcgt taccgccatc agaaatgtct tcaccaacgc cacgcgtggt
     7381 gttgcaggca gttaatactg ttgaaagcac cagaacagaa aagatcgctg caattgtctt
     7441 cttcaccata acgtcttcct tttaggtaat gttatttatg tttgcctata gcaaattaat
     7501 aatagacaac atcgccaaaa atgatggaaa tctgatgatt tttcggaaga gaagaaaaat
     7561 tagctggcag cgcgggagat ggagttgccg agatgtttga tgtcttcgcc gaaaccgcga
     7621 gcggtgttac agcccgtgag cagcgtgctg gcaagcaaaa caagaacgat aaggcgtttc
     7681 atcatctctg acctgaataa gtgatggtgc agcctgcagg ccgcaccaca accgcattac
     7741 ttcacgcgag agacgtattc accagagcgg gtatccactt tgatgacttc gccgatttgt
     7801 acgaacagcg gaactttaac cacagcgcca gtagacaggg tagccggttt gccgccagta
     7861 cctgcggtat cacctttcag gcccggatcg gtatcaacga tttccagttc aacgaagttc
     7921 ggcggagtaa cggagatcgg ctgaccattc cacagagtta cgatacactc tgcctgatcc
     7981 agcagccatt tagcgttgtc gccaattgct tttgcatcag cagacagctg ctcgaaagtt
     8041 tcgttgttca tgaagtgcca gaactcaccg tcgttgtaca ggtaagtcag gttcatatcg
     8101 acaacatcag cgccttcagc ggaatcagta gatttgaagg ttttttctac gcgagtaccg
     8161 gtcagcagac gacgcagttt aacgcgagca aatgcctggc ctttacccgg ttttacgaat
     8221 tcactcgctt caaccgcgta aggttcgccg tctaacatga ttttaagacc agcacgaaaa
     8281 tcgttgctat agtacgttgc cataaggccc tctgaaattt gttaattggt agctaagcca
     8341 caaaatggcg catattgtaa ccctaaatac cccatccaga gaagattggt taacgcaact
     8401 tgccgatgtt gtgaccgatc ctggtgaact tctgcgtctt ttgaatatag acgcggacga
     8461 aaaactgttg gccggacgca gcgccaaaaa gctgtttgcc ctgcgtgtgc cccgctcatt
     8521 tatcgatcgc atggagaaag gcaatccgga cgatcctatt ttgcgtcagg tacttacctc
     8581 gcaagatgag tttgtcgtcg cgtccggatt ctccaccgac ccactggaag aacagcacag
     8641 cgtagtgcct ggtttgttgc ataaatacca caaccgggcg cttttgctgg tcaaaggcgg
     8701 ctgcgcggta aattgccgct attgcttccg tcgccacttc ccctatgccg aaaatcaggg
     8761 caacaagcgt aactggcaga ctgcacttga gtatgttgct gcgcatccgg aactggacga
     8821 gatgattttc tccggcggcg atccgctgat ggcgaaagat cacgagctgg actggttgct
     8881 cacacaactg gaagccatcc cgcatatcaa acgtctgcgg attcacagcc gtctgccgat
     8941 tgtgatcccg gcacgtatca ccgaggcgct ggttgaacgc tttgcccgtt ctacgctgca
     9001 aatcttgctg gtgaatcaca tcaatcatgc caatgaggta gatgaaacgt tccgtcaggc
     9061 gatggctaaa ttgcgccgtg tcggtgtcac cctgcttaac cagagcgttc tgttacgtgg
     9121 tgtgaacgat aacgcacaaa cgttggcaaa cctgagtaat gcgttgttcg atgccggcgt
     9181 gatgccgtat tacctgcatg tgctagataa agtacagggc gcggcgcatt ttatggtgag
     9241 tgatgacgaa gcacggcaga ttatgcgtga gttgctgaca ttggtgtcgg gatatctggt
     9301 gccgaaactg gcgcgagaaa ttggcggcga acccagcaaa acgccgctgg atctccagct
     9361 acgccagcag taactgatta aacagggaag ttaaacatta gcctgatgcg ctacgcttat
     9421 caggcctaca ttgtgcattg aaattgaatg gatttgcaat gaactgtagg ccggataagg
     9481 cgtttacgcc gcatccggca agagcaacaa acgcaagtac agcaacctgt cattaaactg
     9541 ggaaatttaa tctttgcccg ttgcataacc tttaattatt catatacatt gctgttgtta
     9601 ctctttatat agccaacacc aataattaac ctttcttttc tgtttatata gagtgagtac
     9661 ggctatttat gttattttaa caactattac taccaagccc atttatctgg cttataaatt
     9721 aaaattaatt atttaattga gaaatggtta gggagaacct acatggccat aagcatcaag
     9781 ggtgttaata ccggtgttat tcgcaagagt aataatttca ttgcactggc gctgaaaatt
     9841 aaagaacctc gcaataaaga atcgttgttc tttatgtctg tcatggaact gcgcgacctg
     9901 ctgattgcgc tggaaagtcg tctgcatcaa aagcataaac tgaatgctgc tgcacgcctt
     9961 cagtatgagc aagctcgcga taaagtcatt aagaaaatgg cagaaaacat cccagaaatt
    10021 ctggttgatg agcttaaaaa tgccgatatt aatcgtcggg ttaacacgct ggaacttacc
    10081 gataatcaag gcgaaaatct taccttcgta ttaacgctac atgacggtag caaatgtgag
    10141 ttagtcgtca atgagttgca gattgaaatg ctggcgcgag caatcattca tgcaattaat
    10201 aatgcggaaa tgcgtgaact ggcattacgt attacctcac tgttagattt cctgccgtta
    10261 tacgacgttg actgccagga caatggcaat ctggaatatg acacctattc ccaaccggaa
    10321 tggaaacata acctgttcga ccattatctg gcggtgcttt accgctttaa agatgaaagt
    10381 ggcaaagaac aatttagcgg cacagtggtg aaaacgcgtg aagcaacgcc gggtaaagaa
    10441 atagaggcga tcactcgccg aatgcttgat ttcagcccac gactgaaaaa actcgcgggt
    10501 gtgccatgtc aggtctatgt tcgcaccgta gcggcgaata acgcccaacc gctaacccag
    10561 gatcaatgct tacgcgcgct gcatcactta cgtgttcaat ccaccagcaa aactgcacca
    10621 caggcgaaat aatctcatct ttgctaatgc gaggctggag ccatccagcc tgaaatgact
    10681 gataacgtga tcgttggtca tgccggatgc gacgtaaacg ccttatccgg cctacaaatt
    10741 ctcaagcatt caatcagttg caatgcctgc tgtaggcctg ataagcgaag cgcatcaggc
    10801 aataatacgt ttgtcaccca gcctctggat cacatcagtt cgggcactta taaacctgac
    10861 cgataatctg gctatccgtc gggacaaaac tggacaacat tccctgcgac gggctgctga
    10921 tgccataaat cacgttaccg cccattgcag ccgcctggtt gcgcagatcg tttgctgcgc
    10981 cgcgcataga accgccctct tctccgtgtt gcccggaaag ccagttgctt tgcttacctg
    11041 tcgcagtacc aatcagctgg cactctgcgc ctggctgctc gtccacaatg cgtacactct
    11101 gaccggcagc actcaattcg ttgctggagc tacaacccgc catcagtagc gcggcaccga
    11161 caatccctgc taagtatttt acgtgcatgt tattccccat aatcaatgag ctggacgcca
    11221 ctcgcgtcgt ccgtgtctga atcttatact agaaagatga ccaaaagaaa aacccccaga
    11281 catttctgcc cgggggtttg tttatttctg cgaggtgcag ggcaattaca tcatgccgcc
    11341 catgccaccc atgccgccca taccgccagc agcgcctaag tcagctgcat catttttcgg
    11401 caggtcggta accatgcact cggtggtgat catcaggcca gccacagaag ccgcgtactg
    11461 cagagcagaa cgggttactt tggttgggtc caggataccc atgtcgatca tgttgccgta
    11521 ttcttcggtt gctgcgttgt aaccgtagtt gccgtcgccg cctttaacgg tgttagcaac
    11581 aacagacggt tcttcgccgc agttcaggac gatctgacgc agcggagctt ccattgcacg
    11641 cagtgcaact ttgataccca cgttctggtc ttcgttctga ccacgcaggt cagccagttt
    11701 agacgctacg cggatcagcg caacaccacc accagcaacc acgccttctt ctaccgcagc
    11761 acgggtcgcg tgcagggcgt cttcaacgcg tgcttttttc tctttcattt caacttcggt
    11821 agcagcacct actttgataa ctgcaacgcc gcctgccagt ttcgctacgc gttcctgcag
    11881 tttttcacgg tcgtagtcag aagttgcttc ttcaatctgc tgacggatct gagcaacacg
    11941 gccctggatt gcagcttctt cacccacgcc atcgatgata gtggtggtgt ctttgttgat
    12001 cacaacacgt ttagcctgac ccaggtcttc cagggttgct ttttccagtt ccataccgat
    12061 ctcttcagag atcacggtac cgccagtcag ggttgcgata tcctgcagca tagctttacg
    12121 acgatcgccg aagcccggag ctttaactgc agcaactttc acgatgccac gcatggtgtt
    12181 aacaaccaga gttgccagcg cttcgccttc tacatcttca gcgatgatca gcagcggttt
    12241 gcctgctttg gcaacggctt ccagaaccgg cagcatttcg cggatgttgg agattttctt
    12301 gtcagccagc aggatgaacg ggctttccag ttctactgcg ccagtttccg gcttgttgat
    12361 gaagtaagga gacaggtagc cacggtcgaa ctgcatacct tcaaccacgt ccagttcgtc
    12421 ctgcagaccg gtaccgtctt caacggtgat aacgccttct ttaccgactt tgtccatcgc
    12481 ttcagcgatc agtttaccta cggtttcgtc ggagttagcg gagatggtac caacctgagc
    12541 aatcgcttta gagtcagagc acggtacgga cagcgctttc agttcttcaa ctgcagcggt
    12601 aacggctttg tcgataccac gtttcaggtc catcgggttc atgcccgcag caacagcttt
    12661 cagaccctca gtgatgatag cctgagccag tacagttgca gtggtggtac cgtcgcctgc
    12721 agcgtcgttc gctttagagg caacttcttt caccatctgc gcacccatgt tttcgaactt
    12781 gtcttccagt tcgatttcac gagcaacgga aacaccatct ttggtgatgg tcggtgcacc
    12841 gaaagattta tccagaacta cgttacggcc tttcggaccg agggtaactt tcactgcatc
    12901 tgccagtacg tttacgccgc gcagcatttt cacacgagcg tcgttaccga attttacgtc
    12961 tttagctgcc attatcttta ttccttaaat tcgtatgttc agtgtcgtgc gaggattacg
    13021 cttcaacaat tgccagaatg tcgctttcgg acatgatcaa cacttcttca ttgtcgatct
    13081 tctcagattt cacaccgtag ccatcgttga aaataacgat gtcgccaact ttcacatcca
    13141 gcggcttcac ttcgccattt tcaaggatac ggccattgcc gacagccagc acttcgccgc
    13201 gggtggattt agccgctgca gagccggtca gaacgatgcc gccagcagat ttagtttcaa
    13261 cttctttacg cttgacgatc acgcgatcat gcaatggacg aatattcatt gataactctc
    13321 ctttgagaaa gtccgtatct gttatgggtg acgccggagc ttacgtggtt tcccggctgg
    13381 tgaccagaga aatggggatg aggattcgcc ccttcaaggg ggaaaaagaa aaaaaattct
    13441 gatcagcaca aaatcgggtg aaaaccctga ttcacctcac atttcatcgc aatttcttca
    13501 tcgccgtcat aagcgaatct gattgtgcta ccatcgaaaa tctacgcagt tgcccaaaat
    13561 ttgggcgcaa tcggatatca acccatgagt ggactcaaac aagaactggg gctggcccag
    13621 ggcattggcc tgctatcgac gtcattatta ggcaccggcg tgtttgccgt tcctgcgtta
    13681 gctgcacagg tagcaggcaa taacagcctg tgggcgtggc ccgttttgat tatcttagtg
    13741 ttcccgattg cgattgtgtt tgcgattctg ggtcgccact atcccagcgc aggcggtgtc
    13801 gcgcacttcg tcggtatggc gtttggttcg cggcttgagc gagtcaccgg ctggttgttt
    13861 ttatcggtca ttcccgtggg tttgcctgcc gcgctacaaa ttgccgccgg gttcggccag
    13921 gcaatgtttg gctggcatag ctggcaactg ttgttggcag aactcggtac gctggcgctg
    13981 gtgtggtata tcggtactcg cggtgccagt tccagtgcta atctacaaac cgttattgcc
    14041 ggacttatcg tcgcgctgat tgtcgctatc tggtgggcgg gcgatatcaa acctgcgaat
    14101 atccccttcc cggcacctgg taatatcgaa cttaccgggt tatttgctgc gttatcagtg
    14161 atgttctggt gttttgtcgg tctggaagca tttgcccatc ttgcctcgga atttaaaaat
    14221 ccagagcgtg attttcctcg cgcattgatg attggtctgc tgctggcagg attagtctac
    14281 tggggctgta cggtagtcgt cttacacttc gacgcctatg gtgaacaaat ggccgcggca
    14341 gcatcgcttc caaaaattgt agtgcaactg ttcggtgtag gagcgttatg gattgcctgc
    14401 gtaattggct atctgacctg ctttgccagt ctcaacattt atatacagag cttcgcccgc
    14461 ctggtctggt cgcaggcgca acataatcct gaccactacc tggcacgcct ctcttctcgc
    14521 catatcccga ataatgccct caatgcggtg ctcggctgct gcgtggtgag cactttggtg
    14581 attcatgctt tagagatcaa tctggacgct cttattattt atgccaatgg catctttatt
    14641 atgatttatc tgttatgcat gctggcaggc tgtaaattat tgcaaggacg ttatcgacta
    14701 ctggcggtgg ttggcgggct gttatgcgtt ctgttactgg caatggtcgg ctggaaaagt
    14761 ctctatgcgc tgattatgct ggcggggtta tggctgtttc tgccaaaacg aaaaacgccg
    14821 gaaaatggca taaccacata atccggcgtt tcgacattaa tcctagcgat cgtctttatg
    14881 atcaaggcgg tcgcggtcat catcctttcg ctggtactca ccatcaaaag tattaccgcc
    14941 accggtcccg gcgctaaaac cgccgcctgg catgcgagaa aagcgcaaat gtggcatcaa
    15001 cttcactgtc agatgctttt gcaccggcgg caataaaagc agaagaccga ggaagtcggt
    15061 gaaaaagccc ggtaataaaa gcagcaaacc ggcaataatc agcgaaacac ttttaatcat
    15121 ctccgccgca gggttttcac ccgccgccat tttttgctgc atcagcacga aattcttaaa
    15181 gccctggtta cgtaccagtg acataccgat aactgacgtg aatataacca gcacgagggt
    15241 cagcaatacc cccaatacat gggcaacctg aataaagatt gaaatctcaa tatagacata
    15301 aaggaaaatg gcaataaaag gtaaccagcg caaaggtttc tcctgtaata gcagccggtt
    15361 aaacccggct acctgaatgg gttgcgaatc gcgttcagct tatattgtgg tcattagcaa
    15421 aatttcaaga tgtttgcgca actatttttg gtagtaatcc caaagcggtg atctatttca
    15481 caaattaata attaaggggt aaaaaccgac acttaaagtg atccagatta cggtagaaat
    15541 cctcaagcag catatgatct cgggtattcg gtcgatgcag gggataatcg tcggtcgaaa
    15601 aacattcgaa accacatata ttctgtgtgt ttaaagcaaa tcattggcag cttgaaaaag
    15661 aaggttcaca tgtcaaacaa cattcgtatc gaagaagatc tgttgggtac cagggaagtt
    15721 ccagctgatg cctactatgg tgttcacact ctgagagcga ttgaaaactt ctatatcagc
    15781 aacaacaaaa tcagtgatat tcctgaattt gttcgcggta tggtaatggt taaaaaagcc
    15841 gcagctatgg caaacaaaga gctgcaaacc attcctaaaa gtgtagcgaa tgccatcatt
    15901 gccgcatgtg atgaagtcct gaacaacgga aaatgcatgg atcagttccc ggtagacgtc
    15961 taccagggcg gcgcaggtac ttccgtaaac atgaacacca acgaagtgct ggccaatatc
    16021 ggtctggaac tgatgggtca ccagaaaggt gaatatcagt acctgaaccc gaacgaccat
    16081 gttaacaaat gtcagtccac taacgacgcc tacccgaccg gtttccgtat cgcagtttac
    16141 tcttccctga ttaagctggt agatgcgatt aaccaactgc gtgaaggctt tgaacgtaaa
    16201 gctgtcgaat tccaggacat cctgaaaatg ggtcgtaccc agctgcagga cgcagtaccg
    16261 atgaccctcg gtcaggaatt ccgcgctttc agcatcctgc tgaaagaaga agtgaaaaac
    16321 atccaacgta ccgctgaact gctgctggaa gttaaccttg gcgcaacagc aatcggtact
    16381 ggtctgaaca cgccgaaaga gtactctccg ctggcagtga aaaaactggc tgaagtcact
    16441 ggcttcccat gcgtaccggc tgaagacctg atcgaagcga cctctgactg cggcgcttat
    16501 gttatggttc acggcgcgct gaaacgcctg gctgtgaaga tgtccaaaat ctgtaacgac
    16561 ctgcgcttgc tctcttctgg tccacgtgcc ggcctgaacg agatcaacct gccggaactg
    16621 caggcgggct cttccatcat gccagctaaa gtaaacccgg ttgttccgga agtggttaac
    16681 caggtatgct tcaaagtcat cggtaacgac accactgtta ccatggcagc agaagcaggt
    16741 cagctgcagt tgaacgttat ggagccggtc attggccagg ctatgttcga atccgttcac
    16801 attctgacca acgcttgcta caacctgctg gaaaaatgca ttaacggcat cactgctaac
    16861 aaagaagtgt gcgaaggtta cgtttacaac tctatcggta tcgttactta cctgaacccg
    16921 ttcatcggtc accacaacgg tgacatcgtg ggtaaaatct gtgccgaaac cggtaagagt
    16981 gtacgtgaag tcgttctgga acgcggtctg ttgactgaag cggaacttga cgatattttc
    17041 tccgtacaga atctgatgca cccggcttac aaagcaaaac gctatactga tgaaagcgaa
    17101 cagtaatcgt acagggtagt acaaataaag aaggcacgtc agatgacgtg ccttttttct
    17161 tgtgagcagt aacttaaaaa taacaatcta atatcaactt gttaaaaaac aaggaaggct
    17221 aatatgctag ttgtagaact catcatagtt ttgctggcga tcttcttggg cgccagattg
    17281 gggggaatag gtattggttt tgcaggcgga ttgggggtgc tggttcttgc cgctattggc
    17341 gttaaacccg gtaacatccc gttcgatgtc atttccatta tcatggcggt tatcgccgct
    17401 atttctgcca tgcaggttgc tggcggtctg gactatctgg ttcatcagac agaaaagctg
    17461 ctgcgccgta acccgaaata catcacaatc ctcgcaccga tcgtgaccta tttcctgaca
    17521 atctttgctg gtactggcaa catctctctg gcgacactgc cagttatcgc tgaagttgcg
    17581 aaggaacaag gcgttaaacc ttgccgtccg ctgtctactg cagtggtatc cgcgcagatt
    17641 gcgatcaccg catcgccaat ctcagcggca gtggtttaca tgtcttccgt gatggaaggt
    17701 catggcatca gctacctcca tctgctctcc gtggtcatcc cgtccaccct gctggcggtt
    17761 ctggtgatgt ccttcctggt cactatgctg ttcaactcca aactctctga cgatccgatt
    17821 tatcgcaagc gtctggaaga gggcctggtt gaactgcgcg gtgaaaagca gattgaaatc
    17881 aaatccggtg caaaaacgtc cgtctggctg ttcctgctgg gcgtagttgg cgtggttatc
    17941 tatgcaatca tcaacagccc aagcatgggt ctggttgaaa aaccgctgat gaacaccacc
    18001 aacgcaatcc tgatcatcat gctcagcgtt gcaactctga ccaccgttat ctgtaaagtc
    18061 gataccgaca acatcctcaa ctccagcacc ttcaaagcag gtatgagcgc ctgtatttgt
    18121 atcctgggtg ttgcgtggct gggcgatact ttcgtttcca acaacatcga ctggatcaaa
    18181 gataccgctg gtgaagtgat tcagggtcat ccgtggctgc tggccgtcat cttcttcttt
    18241 gcttctgctc tgctgtactc tcaggctgca accgcaaaag cactgatgcc gatggctctg
    18301 gcactgaacg tttctccgct gaccgctgtt gcttctttcg ctgcggtgtc tggtctgttc
    18361 attctgccga cctatccgac gctggttgct gcggtacaga tggatgacac gggtacaacc
    18421 cgtatcggta aattcgtctt caaccatccg ttcttcatcc cgggtactct gggtgttgcc
    18481 ctggccgttt gcttcggctt cgtgctgggt agcttcatgc tgtaatgacc aatcgcgggg
    18541 cgttcacgcc ccgctttctt tcccgccgac taacatcctt cccccgtccg ttgtatagtg
    18601 acctctctct tgcggttcca tctgttcttg cgaggtgttt atgcttgatg aaaaaagttc
    18661 gaataccgcg tctgtcgtgg tgctatgtac cgcaccggat gaagcgacag cccaggattt
    18721 agccgccaaa gtgctggcgg aaaaactggc ggcctgcgcg accttgatcc ccggcgctac
    18781 ctctctctat tactgggaag gtaagctgga gcaagaatac gaagtgcaga tgattttaaa
    18841 aactaccgta tctcaccagc aggcactgct ggaatgcctg aagtctcatc atccatatca
    18901 aaccccggaa cttctggttt tacctgttac acacggagac acagattacc tctcatggct
    18961 caacgcatct ttacgctgat cctgctactt tgcagcactt ccgtttttgc cggattattc
    19021 gacgcgccgg gacgttcaca ttttgtcccc gcggatcaag cctttgcttt tgattttcag
    19081 caaaaccaac atgacctgaa tctgacctgg cagatcaaag acggttacta cctctaccgt
    19141 aaacagatcc gcattacgcc ggaacacgcg aaaattgccg acgtgcagct gccgcaaggc
    19201 gtctggcatg aagatgagtt ttacggcaaa agcgagattt accgcgatcg gctgacgctt
    19261 cccgtaacca tcaaccaggc gagtgcggga gcgacgttaa ctgtcaccta ccagggctgt
    19321 gctgatgccg gtttctgtta tccgccagaa accaaaaccg ttccgttaag cgaagtggtc
    19381 gccaacaacg cagcgtcaca gcctgtgtct gttccgcagc aagagcagca caccgcgcaa
    19441 ttgccctttt ccgcgctctg ggcgttgttg atcggtattg gtatcgcctt tacgccatgc
    19501 gtgctgccaa tgtacccact gatttctggc atcgtgctgg gtggtaaaca gcggctctcc
    19561 actgccagag cattgttgct gacctttatt tatgtgcagg ggatggcact gacttacacg
    19621 gcgctgggtc tggtagttgc cgccgcaggg ttacagttcc aggcggcgct acagcaccca
    19681 tacgtgctca ttggcctcgc catcgtcttt accttgctgg cgatgtcaat gtttggcttg
    19741 tttactctac aactcccctc ttcgctgcaa acacatctca cattgatgag caatcgccaa
    19801 cagggcggct cacctggcgg tgtgtttgtt atgggggcga ttgccggact gatctgttca
    19861 ccatgcacca ccgcaccgct tagcgcgatt ctgctgtata tcgcccaaag cgggaacatg
    19921 tggctgggcg gcggcatgct ttatctctat gcgttgggca tgggcctgcc gctgatgcta
    19981 attaccgtct ttggtaaccg cttgctgccg aaaagcggcc cgtggatgga acaagtcaaa
    20041 accgcgtttg gttttgtgat cctcgcactg ccgatcttcc tgctggagcg agtgattggt
    20101 gatatatggg gattacgctt gtggtcggcg ctgggtgtcg cattctttgg ctgggccttt
    20161 atcaccagcc tacaggccaa acgcggctgg atgcgcgtgg tgcaaataat cctgctggca
    20221 gcggcattgg ttagcgtgcg cccacttcag gattgggcat ttggtgcgac gcataccgcg
    20281 caaactcaga cgcatctcaa ctttacacaa atcaaaacgg tagatgagtt aaatcaggcg
    20341 ctcgttgaag ccaaaggcaa accggtgatg ttagatcttt atgccgactg gtgcgtcgcc
    20401 tgtaaagagt ttgagaaata caccttcagc gacccgcagg tgcaaaaagc gttagcagac
    20461 acggtcttac ttcaggccaa cgtcactgct aacgacgcac aagatgtggc gctgttaaag
    20521 catcttaatg tccttggact accgacaatt ctcttttttg acggacaagg ccaggagcat
    20581 ccacaagcac gcgtcacggg ctttatggat gctgaaacct tcagcgcaca tttgcgcgat
    20641 cgccaaccgt gaacgacact tgcagtggga aagacggagg aaaatagcgt gcaacgtgaa
    20701 gatgtactgg gagaagccct gaaattatta gaattacaag ggattgccaa caccacgctg
    20761 gagatggttg ctgaacgtgt ggattatcca ctggacgagc tacgccgctt ctggccagac
    20821 aaagaggcaa tcctctacga tgcgctgcgt tatcttagcc aacagataga tgtctggcgt
    20881 cgtcagctga tgcttgacga aacgcaaacc gccgaacaaa agctgctggc acgttatcag
    20941 gcgttatcgg agtgcgttaa aaacaaccgc tatccgggct gtctatttat cgctgcctgt
    21001 acgttttatc ccgaccctgg ccaccctatt catcaactgg ccgatcagca aaaaagcgcg
    21061 gcctatgatt tcacccacga actgttaacc acgctggaag ttgacgatcc ggcgatggta
    21121 gcaaagcaga tggaactggt gctggaaggc tgtttaagcc gaatgctggt gaatcgtagc
    21181 caggcggatg tcgacaccgc acatcggctg gcggaagata tcctgcgctt cgcccgctgc
    21241 cgccagggtg gtgcactgac ctgacagaaa cacagaaaag aagcgatttg ccgcaatctt
    21301 aagcagttga atcgctttta ctgaaattag gttgacgaga tgtgcagatt acggtttaat
    21361 gcgccccgtt gcccggatag ctcagtcggt agagcagggg attgaaaatc cccgtgtcct
    21421 tggttcgatt ccgagtccgg gcaccaaatt catataaacg gacctccacg gaggtccgtt
    21481 tttcgtttca ggacgccacg atttaagcgt tctgcctcca aatcaattct accgaactca
    21541 accagattct ccccacatca ccagcatttt gcgggtatat cccaattcgg gaaaatttgt
    21601 tcctgagcga tagcactgac tgacgtgaaa tgtcgtgcgg ccccgtgatg ttgttgaacg
    21661 tcaaatgacg tcatcaggag cgtaacgcat ccataaagca caacaccggt ctgaacggcg
    21721 actgatgaga ttttctgaat gagaacaaag agaaatgtat cagtccgctt gctcatgcaa
    21781 agactaacaa tccattaaaa tagtaagcgc tccggacaat tttccatgga ttattttcta
    21841 cacatttttc tttggcaaag atgatgaatt ttgatggtaa ggaatattac ttctggttct
    21901 cagtaaaatc ctttcgtaat actatgtaat caagaagttt atggctggta aaaataacgt
    21961 cttgcattca ccaataacat gtaaataaac ccatctattg atggtaaaaa taggtcatgg
    22021 caattatcat tgcaccattc ccttttcgaa tgagtttcta ttatgcaaca acctgtagtt
    22081 cgcgttggcg aatggcttgt tactccgtcc ataaaccaaa ttagccgcaa tgggcgtcaa
    22141 cttacccttg agccgagatt aattgatctt ctggttttct ttgctcaaca cagtggcgaa
    22201 gtacttagca gggatgaact tatcgataat gtctggaaga gaagtattgt caccaatcac
    22261 gttgtgacgc agagtatctc agaactacgt aattcattaa aagataatga tgaagatagt
    22321 cctgtctata tcgctactgt accaaagcgc ggctataaat gaatggtgcc ggttatctgg
    22381 tacagcgaag aagagggaga ggaaataatg ctatcttcgc ctccccctat accagaggcg
    22441 gttcctgaca caaattctcc ctcccacagt cttaccattc aaaacaccgc aacgccacct
    22501 gaacaatccc cagttaaaag caaacgattc actacctttt gggtatggtt tttttcctgt
    22561 tgtcgttagg tatctgtgta gcactggtag cgttttcaac tcttgataca cgtcttccta
    22621 tgagcaaatc gcgtattttg ctcaatccac gcgatattga cattaatatg gtaaataaaa
    22681 gttgtaacag ctggagttcc ccgtatcagc tctcttacgc gataggcgtg ggtgatttgg
    22741 tggcgacatc acttaacacc ttctccacct ttatggtgca tgacaaaatc aactacaaca
    22801 ttgatgaacc gagcagttcc ggtaaaacat tatctattgc ctttgttaat cagcgccaat
    22861 accgtgctca acaatgcttt atgtcggtaa aattagtaga caatgcagat ggttcaacca
    22921 tgctggataa acgttatgtc atcactaacg gtaatcagct ggcgattcaa aatgatttac
    22981 tggagagttt atcaaaagcg ttaaaccaac cgtggccaca acgaatgcag gagacgctcc
    23041 agcaaatttt gcctcatcgt ggtgcgttat taactaattt ttatcaggct catgattatt
    23101 tactgcatgg cgatgacaaa tcattgaatc gtgccagtga attattaggt gagattgttc
    23161 aatcatcccc agaatttacc tacgcgagag cagaaaaagc attagttgat atcgtgcgcc
    23221 attctcaaca ccctttagat gaaaaacaat tagcagcact gaacacagaa atagacaaca
    23281 ttgttacact gccggaattg aacaacctgt ccattatata tcaaataaaa gcggtcagtg
    23341 ctctggtaaa aggtaaaata gatgagtctt accaggcgat aaatactggc attgatcttg
    23401 aaatgtcctg gctaaattat gtgttgcttg gcaaggttta tgaaatgaag gggatgaacc
    23461 gggaagcagc tgatgcatat ctcaccgcct ttaatttacg cccaggggca aacacccttt
    23521 actggattga aaatggtatt ttccagactt ctgttcctta tgttgtacct tatctcgaca
    23581 aatttctcgc ttcagaataa gtaactccgg gttgatttat gctctggaat atttgttgtt
    23641 gagtttttgt atgttc
//
LOCUS       DAKISA010000066        23494 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.066, whole genome shotgun sequence.
ACCESSION   DAKISA010000066 DAKISA010000000
VERSION     DAKISA010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23494
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.066"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            106..1611
                     /locus_tag="OVW45_002654"
     CDS             106..1611
                     /locus_tag="OVW45_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCV5527064.1"
                     /translation="MQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVCN
                     ILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLILV
                     GWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSLG
                     YGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAWR
                     PDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLES
                     SRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNPV
                     NSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISPQ
                     ETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENIR
                     NGKYPYIVDAFMVTRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
     gene            1627..2337
                     /locus_tag="OVW45_002655"
     CDS             1627..2337
                     /locus_tag="OVW45_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5527065.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            2340..3107
                     /locus_tag="OVW45_002656"
     CDS             2340..3107
                     /locus_tag="OVW45_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5527066.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
     gene            3110..3715
                     /locus_tag="OVW45_002657"
     CDS             3110..3715
                     /locus_tag="OVW45_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5527067.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(3767..5272)
                     /gene="glpD"
                     /locus_tag="OVW45_002658"
     CDS             complement(3767..5272)
                     /gene="glpD"
                     /locus_tag="OVW45_002658"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCV5527068.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDRM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLEEYTQQKLSLAS"
     gene            5462..5788
                     /gene="glpE"
                     /locus_tag="OVW45_002659"
     CDS             5462..5788
                     /gene="glpE"
                     /locus_tag="OVW45_002659"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCV5527069.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            5833..6663
                     /gene="glpG"
                     /locus_tag="OVW45_002660"
     CDS             5833..6663
                     /gene="glpG"
                     /locus_tag="OVW45_002660"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCV5527070.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLEKPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            6680..7438
                     /locus_tag="OVW45_002661"
     CDS             6680..7438
                     /locus_tag="OVW45_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCV5527071.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(7420..9018)
                     /gene="rtcR"
                     /locus_tag="OVW45_002662"
     CDS             complement(7420..9018)
                     /gene="rtcR"
                     /locus_tag="OVW45_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCV5527072.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDTRSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENINLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            9207..10433
                     /gene="rtcB"
                     /locus_tag="OVW45_002663"
     CDS             9207..10433
                     /gene="rtcB"
                     /locus_tag="OVW45_002663"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCV5527073.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVFARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            10437..11453
                     /gene="rtcA"
                     /locus_tag="OVW45_002664"
     CDS             10437..11453
                     /gene="rtcA"
                     /locus_tag="OVW45_002664"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCV5527074.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRLGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAVVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(11496..14201)
                     /gene="malT"
                     /locus_tag="OVW45_002665"
     CDS             complement(11496..14201)
                     /gene="malT"
                     /locus_tag="OVW45_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HCV5527075.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSWICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKTFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            14812..17205
                     /gene="malP"
                     /locus_tag="OVW45_002666"
     CDS             14812..17205
                     /gene="malP"
                     /locus_tag="OVW45_002666"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCV5527076.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            17215..19299
                     /gene="malQ"
                     /locus_tag="OVW45_002667"
     CDS             17215..19299
                     /gene="malQ"
                     /locus_tag="OVW45_002667"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCV5527077.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVKA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAVAKKK"
     gene            complement(19344..20660)
                     /gene="gntT"
                     /locus_tag="OVW45_002668"
     CDS             complement(19344..20660)
                     /gene="gntT"
                     /locus_tag="OVW45_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCV5527078.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIDSIKVGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(21021..21596)
                     /gene="nfuA"
                     /locus_tag="OVW45_002669"
     CDS             complement(21021..21596)
                     /gene="nfuA"
                     /locus_tag="OVW45_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCV5527079.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(21655..22338)
                     /gene="gntX"
                     /locus_tag="OVW45_002670"
     CDS             complement(21655..22338)
                     /gene="gntX"
                     /locus_tag="OVW45_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCV5527080.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            22376..23146
                     /gene="bioH"
                     /locus_tag="OVW45_002671"
     CDS             22376..23146
                     /gene="bioH"
                     /locus_tag="OVW45_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCV5527081.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPRSKSYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            complement(23175..>23494)
                     /locus_tag="OVW45_002672"
     CDS             complement(23175..>23494)
                     /locus_tag="OVW45_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCV5527082.1"
                     /translation="LMGLVEQMACLLSSGYANDRQIKGLFNYILQTGDAVRFNDFIDG
                     VAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADIMRFTGVSEEELAA
                     ASQ"
BASE COUNT         5636 a         5832 c         6355 g         5671 t
ORIGIN      
        1 ccaaagggtc ccgttttttt ccgccatcat ttcggaaaag tgtccagaag gggcaagggg
       61 actcggggag tataatttca ccattcaaaa agaatggaaa gaataatgca aaacagaaaa
      121 tggattttgt cctcgctggt aatgactttt ttcggcatcc ccatactgac gcaatttttg
      181 gcggcggttg ttgctatgct gggtgtcgga cttgccggta ttattgaagt ttgtaatatc
      241 cttatcacgc caacaagtta ccttctgctc aacattttta tgctggcgct gggcgcatta
      301 atgctatttt tctcggggcg agtatgggca ggcgatagtg caccagaaaa aagagaaata
      361 gccgtctggc gacaatgtct ttttttagta cccggtttat taatcctggt tggctggata
      421 atcgcgctgc atctggcaga ttatcaattt caccagatgg gatcgggttg gttggctgat
      481 cttatgctcc cctggctggg cgttttgtta gtctcagtag tcggaggtga gtactggtgg
      541 atagtcatta tacccgttgg cgcgcatatc agtttttcgc tggggtacgg ccggccaacc
      601 agacatcctt taaccggtac atccgggcta cgttgccgta attccctcct gtttatcctt
      661 ctcatgcttg gttttgtcgc tggttatcag ggttatttat ataaacagct caatcccggt
      721 gtcggtgtgc gcgaaaatat tgatacctgg gcctggcgac ccgataaact caataatcaa
      781 ctgacaccac tgcgtggtaa accgcaaatt cagtttacgc aaaactggcc gcgactcgat
      841 ggtgctacgg cggcgtaccc aatttatgcc tctgcatttt atgctttaag cgttataccg
      901 gaagattttc acaccaggga atatctggag agctctcgta cccccgatgc gtataacagg
      961 attgttaaag gtgatgctga tattattttt gtggcgcaac cttccggtgg gcagaaaaaa
     1021 cgcgctgagg aatcgggcgt tactttgctg tacacgcctt ttgcccgtga agcgtttgtt
     1081 ttcatcgtca atgcggataa tccggttaat tccctgactg aacaacaggt gcgtgacatc
     1141 ttcagtggtg caattaccaa ctggcgtact gttggcggta acgatcagga gatccagacc
     1201 tggcaacgcc cggaagactc tggtagccag acggtgatgc aatcccaggt catgaaaaat
     1261 gtccgcatga tctcgccgca ggaaacagaa gtggcaagta tgatggaggg aatgattaaa
     1321 gtcgttgccg aataccgtaa tacaaacaac gcaataggtt acaccttccg ttattacgca
     1381 acacaaatga acgccgataa aaatataaaa ctgctagcga ttaacggtat tgcaccgact
     1441 gccgaaaaca ttcgcaacgg caaatatccc tatatcgtcg atgcctttat ggtaacgcgg
     1501 gaaaatacga cgtcagagac acaaaaattg ttggagtggt ttttaacgcc gcagggacag
     1561 agtttggtgg aagatgtagg ttatgtcccg atgtataaga cgctacctta aagctggata
     1621 ataaaaatgg aattagttaa tacattattt gcaagccttg ccggtactga tcccttcaca
     1681 ggcgtggata ttacgattgc caactgcaaa tcggcatact gggatgaagg cattgttcag
     1741 caacttatta accaggcctt ggatgaaggg gaaaagttcg tcggtgctga cggtcttgag
     1801 ggattgcttc gatatgatgt cacattaaat attggcttaa cctccagcaa ggtatggccg
     1861 ggattttccc tcgataccgc caccattagc cgtctttgtg cctgtggtgc tgattttgga
     1921 tttgacccct atatcagtga tgtaccagat gttcaatgtg acctgaacac gacaaatgac
     1981 gtcaccgtac aatttacggc gatgctaaat ccggatgagc gggtgataat agcaaagcgc
     2041 ccgcttaaaa aatgtgactc ctggattgag gatgtctata tctttcaggt ctttaaagat
     2101 gcctggaaat ttcataacaa taacagttta cgcggatttc gcgataagca ggccgaactg
     2161 aaactctaca cgcgtcatta ctcagtggaa aattgcgcag aggaaagctg tcgagactgc
     2221 aatagttgca ttcgccccag cttttccctt tcgcgtagcg cccttattcg cctgaacgcg
     2281 gcgaatgctc gttttgtcta tcaaccgttt acacgcgatc agcgggcgag ggggtaacta
     2341 tgcaacccag ccccgccatg caggcattaa ttgagcagat ttatcacacc tttcgtcgtt
     2401 atcgggtgcc gcagaaattt gtggtttgtt gtgaatattg cctgagtcag caggaacaaa
     2461 aggcattacg gagtacatcg ttacgagcga tcccgtattc cttaatcaat gcctggaata
     2521 gtagcccagg gcctgatccg caaaatagcg atgaggtccg ctattttctg ccgcgtttac
     2581 tggaatttgt tgcacagggg cattttgaca atattcacgt agttttttct ctgcgccgta
     2641 tcaaccttgc caacaaagaa aactggcgtg cggatgaacg ggcaatttta cagcgatttg
     2701 cctgccagta tatgactgat tgggtatccg gcgatgaagc tgtcgaatta cagtatatgc
     2761 tggaaatgtt tttccgtgcc gatatcgctc ttgcgccatt gctggatgcc attaattcag
     2821 ttcccggctt ttggtcgacc gtttctcttg cctgtttatt gaacagatat tgtgaggatt
     2881 atatcagaga taaccaggac gatatcgata acgccattac cacccaaatc aatgcctggg
     2941 ctagaaataa tcatccttta ctgaaagaaa gagcgcggca agcgattgag aacccgctaa
     3001 aacagcccga accaatgaca gaatatcagg tctgggaaga tgactggata attgatgagt
     3061 gtttatgcgc gatgtatgac gcatcatccg aatcaccagg aaaataaaaa tgattaaatt
     3121 ccggctttat attccccctg taattctcgg ttttgttatc gtgccattat tggtatggcc
     3181 gacggttatt gtcttagccg tacttatatt cacgttaact tttctggcag aaataatatt
     3241 ctcctttccg ctcctggttg tgcgtatttc tcttcaggaa ttacaacttg agttaatggt
     3301 tgaatatgca ctttttttca gtgtaatggc tggcatcggt tggcaattct cccgcagaac
     3361 gcctcctgaa ttaaaaaaca gactgcattg ctggctggtc ttttcgccgg tctatttctg
     3421 gttaattctc tcgaatttca ttctttatat ttctccagag aaatcagcgt tgctggaaaa
     3481 tatccgaaat ttctttctga cgtttgtctg gcctcccctg aatttttccc ctttttggcc
     3541 gcagccttgg actgattttg tcggcccgat tagtgcacag cttggttttg cattgggata
     3601 ttattgccat tggtgcagaa aaaatagaaa ccagaggaag aagtggggcg attgggtaac
     3661 gtgcttaagt ttggcgattt tagctcaggg gccgttattc aattatttac aatgaatagt
     3721 ctaaggaatc caaatagccc ggttgcactt caaccgggca tcatccttac gacgccagcg
     3781 acaacttctg ctgcgtatat tcctccagcc actgactcac acgagattgt tgatccgcat
     3841 tcagccacat gccttgtttt gtgcgacgcc aaagggcatc gtcggtgcgg cggacccatt
     3901 cgtgatccac caggtatttc agttccgctt cgtagaactc atgaccgaaa tcctccccga
     3961 ggtcgcttat cgcccccgca ttgccgagca gcagctcgct gttgctgccg taagtgcgtg
     4021 cgtaatgacg cgccagcgat tcagtcagga atggatagcg acggcgcaga cgagctgcgt
     4081 aatcgtcgcg gtcgccttca atggcaccac ccggcagcac gctctctttc gtccatgccg
     4141 ggccaatgcc ctgataatac ggcgtcagtt tttccagcgc atgttccgcc agtttgcggt
     4201 aggtggtcaa cttaccgccg aataccgaca gcagcggtgc tttgccgttt tcatcatgga
     4261 tatcaagggt gtaatcacgg gtaatagcct gcggcgagtc ggactcatca tcacacagcg
     4321 gacgcacacc ggagtaggtc cagacgatat cgtcacggct taactgcttt ttaaagtgcg
     4381 tgttatacac tttcagcagg taattgattt cactctcttc aatcttcacc gctttcggat
     4441 cgcctttgta ttccacatcg gtggtgccga tgatggaaaa ctcgtccatc cacgggatca
     4501 cgaacacaat gcgtttatct tcgttttgca gaatgtaggc ctgcttttgg gtatgcacgc
     4561 gcggcaccac aatatggctg cctttgatca ggcgaatgcc ataaggcgaa ggcagatgca
     4621 tcctgtcgtc gaagaactgt ttcacccacg ggccggtggc gttaaccaag ccgcgcgctt
     4681 gccagctata ttttttgccg gtatcgatat cttccgcttc cacaatccac aggccgtttt
     4741 cgcggcgagc agaggtggcg cgagtccgag taagcacttc gccgccttta cgcaccacca
     4801 tctgggcgtt ggcgagtacc agacgggcgt cgtctaccca acagtcagaa tattcgaatc
     4861 cgcgcttaat ttccggtttt aacactgaat ttgcgccaaa acgcaaacca gttgatcccg
     4921 gcaggctggt gcgtttaccc agatgatcgt acataaacag accaatgcga atcatccacg
     4981 ccgggcgcag atgcggacga tgtggcaggc gaaaacgcat cgggaaggcg atatgcggag
     5041 ccattttcag cagcacttca cgttcagcca gcgcctcgct gaccaggcgg aattcatagt
     5101 gctcaaggta gcgcaggcca ccgtgaatga gttttgaact ggcggaagag gtcgcgcaag
     5161 cgagatcctg cgcctccagc atcagcacgg ataaaccgcg tccagcggcg tctgccgcga
     5221 taccagcacc attgatgccg ccacctatca caatcagatc tttggtttcc atgctgccct
     5281 cattcacttt cgttaaagct cataaatgtt cgttatcgaa catattagca aagaatcgcg
     5341 ctttaggtaa cattgaaaaa acattttaga gtgatatgta taacattatg gcgtttatct
     5401 gccgcttcga cgtaaactgt gcggtaaatt tgcccacttg tttgtaaaga aagagagacg
     5461 catggatcag ttcgaatgta ttaacgttgc cgacgcgcac cagaagttgc aggaaaaaga
     5521 ggcggtgctg gtcgatattc gcgatccaca gagtttcgct atggggcatg cggtgcaggc
     5581 tttccattta accaacgaca cgctgggcgc gtttatgcgt gataacgact ttgacactcc
     5641 ggtgatggtg atgtgttatc acggcaatag cagcaaaggc gcggcgcagt atctgctgca
     5701 acagggctac gatgtggtct atagcattga cggcggcttt gaagcctggc aacgtcagtt
     5761 tcccgcagag gtggcgtacg gcgcgtaacg ctttatactg tccccttttg tgtggaataa
     5821 gcgacagcaa cgatgttgat gattacctct tttgctaacc cccgcgtggc gcaggcgttt
     5881 gttgattaca tggcgacgca gggtgttatc ctcacgattc aacaacataa ccaaagcgat
     5941 gtctggctgg cggatgagtc ccaggccgag cgcgtacggg cggagctggc gcgttttctc
     6001 gaaaaaccgg cagatccgcg ttatctggcg gccagctggc aggcaggcca taccggcagt
     6061 ggcctgcatt atcgccgtta tcctttcttt gccgccttgc gtgaacgcgc aggtccggtg
     6121 acctgggtga tgatgatcgc ctgcgtggtg gtgtttattg ccatgcaaat tctcggcgat
     6181 caggaagtga tgttatggct ggcctggcca ttcgatccag cactgaaatt tgagttctgg
     6241 cgttacttca cccacgcgtt aatgcacttc tcgctgatgc atatcctctt taacctgctc
     6301 tggtggtggt atctcggcgg tgcggtggaa aaacgcctcg gtagcggtaa gctaattgtt
     6361 attactctca ttagcgccct gttaagcggc tatgtgcagc aaaaattcag cgggccgtgg
     6421 tttggcgggc tttctggcgt ggtgtatgcg ctgatgggct acgtctggct acgtggtgaa
     6481 cgcgatccgc aaagtggcat ttacctgcaa cgtgggttaa ttatctttgc gctgatctgg
     6541 attgtcgccg gatggtttga tttgtttggg atgtcgatgg cgaacggagc acacatcgcc
     6601 gggttagccg tgggtttagc gatggctttt gttgattcgc tcaatgcgcg aaaacgaaaa
     6661 taattccagg gatttataaa tgaaacaaac acaacgtcac aacggtatta tcgaactggt
     6721 taaacagcag ggttatgtca gtactgaaga gctggtagag catttctccg tcagcccgca
     6781 gactattcgc cgcgacctca atgagctggc ggagcaaaac ctgatcctgc gtcaccatgg
     6841 cggtgcggcg ctgccttcca gttcggttaa cacgccgtgg cacgatcgca aagccaccca
     6901 gaccgaagaa aaagagagga ttgcccgcaa agtggcggag caaatcccca acggctcgac
     6961 actgtttatc gatatcggca ccacgccgga agcggtggcg cacgcgctgc ttaatcacag
     7021 caatttgcgt attgtcacca acaatctcaa tgttgctaac acgttgatgg taaaagaaga
     7081 tttccgcatt attctcgccg gtggcgaatt acgcagccgc gatggcggga tcattggcga
     7141 agcgacgctc gattttatct cccagttccg ccttgatttc ggcattctgg ggataagcgg
     7201 catcgatagc gacggctcgc tgctggagtt cgattaccac gaagttcgca ccaagcgcgc
     7261 cattattgag aactcgcgcc acgttatgct ggttgtcgat cactcgaaat ttggccgtaa
     7321 cgcgatggtc aatatgggca gcatcagcat ggtagatgcc gtctacaccg acgccccgcc
     7381 gccagtaagc gtaatgcagg tgctgacgga ccatcatatt caactggagc tgtgctgatc
     7441 ctgcacggct tcccacgtca gaccaaaacg cgccaggtat ttgcgtagcc gatccgcgtc
     7501 attgacgctg gctttgccct ggcgcgaaac atcaaaaagc tgtcgtccgg ctgcagaaag
     7561 cgactttgcc tggcggcaga tagcgataac gtgttccagt tgcatacggt cgaagagatt
     7621 gatgttttct gcctctgcgc ccagcaacgc cgtaagcgcg gaggggcggc tctcctgcca
     7681 gttatagcgc agacggttta tctcatcttc aaccacgtcc agagtgatgc gtccgctagt
     7741 ggcaaaggtg gccatccgcg tgacgctggc agaaagttcg cgaaagttac cgcgccatgt
     7801 tgcctgggga gaggtcgcaa aagccaacca ggcgcgccgc gcttcggtgt taaaacgcac
     7861 gctgtcgcca gtgagtgtgg cgtggcgctc cacttcataa tcaaggttcg gttcaatatc
     7921 ttcctggcgc tggcgtagac ccggcagggt gaaggtccag agattgatcc gcgcgtacag
     7981 atcttcgcga aatttgcctt cggcaaccag ctggcgcaaa tcgcgcaccg ttccggcgat
     8041 aagctgaaaa tcactgctca cctggcgatc gctgccaaac gggtaaaagg ttttctcttc
     8101 aatggctttt agcagcattg cctgttcgtc tgcgcccagt tcgccaatct catcaagaaa
     8161 caacattccg ccgttggcgc tgcgtaataa accttcacga gattcccgcg ccccggtaaa
     8221 cgcgcctttt acatgaccaa acagcgtcga catggcggta tcgccgcgca gggtggcgca
     8281 gttcacttcc acaaacgcgc cgctaaactg atgccgcgcc tgttttaact cgaagatgcg
     8341 tcgcgccaga aatgacttgc ccgcgccggt tggaccgtta agcagaatcg gcgcgcggga
     8401 tttgatcgcc actttttcga tctgctcaat catgcggttg aagtgggggt tacgcgtggc
     8461 aatgccggac ttaagaaaat caagcgtttg ctggcgttcc tcggcaaagc ggctggcgat
     8521 ggcgttataa cggcttaaat cgagatcgat aatcgtcact tcacctgcgc cgcgcggctg
     8581 ttcttttttg cgcggaggtg aagattgtat cagccgggcg ggcaggtaac gcgcttccgc
     8641 cagcagaaac cagcaaatct gcgcgacgtg ggtgccggtg gtgatgtgaa ttaaatagtc
     8701 ttctttttct ggctgaaact cgtaaccacg ggcgaaatca tgcaggcagg cgtaaacctc
     8761 ttcgaaatcc cacgggttat gcagttcaat ctcaacgccc acgacttctg tttctggcga
     8821 aacgctggcg atatcgcgtt taagcgtttc gaatagcgag cgcgagcgag tgtcgtgcaa
     8881 caattccagc cgatcgatga ctaacgattc ttgctggcat aaacaaagcg tcggacgcca
     8941 tttcgaccag cgctgactgc cgcgtccggc ataatccagc acggtaccga caaagccaaa
     9001 agccactgtt ttacgcatct tagatatcct tataaaagac gatatttcat tatcggtgaa
     9061 ttggttttgt ggaaaatctc cgtttacgga cgtaaagatt taacctattt aaaaacaatg
     9121 aattaaaaaa ttaatctgct tatttaattt tctggcacga cggttgcaat tatcacgaca
     9181 gcaaacaacg aaaagagaaa aacaaaatga attacgaatt actgaccact gaaaatgccc
     9241 cggtaaaaat gtggaccaaa ggcgtgccgg tagaagccga tgcgcgtcag caacttatta
     9301 atacggcgaa gatgccgttt attttcaaac atattgcggt aatgcctgat gtacacctgg
     9361 gtaaaggttc caccattggt agcgtgatcc cgaccaaagg ggcgattatt ccggcggcgg
     9421 tgggcgtgga tattggctgt ggaatgaacg cgctgcgtac cgcgttaacg gcggcagacc
     9481 tgcctgaaaa cctggcggag ctgcgtcaag cgattgaaac ggccgtacca cacgggcgta
     9541 ctaccgggcg ttgtaaacgt gataaaggcg catgggaaaa cccgcctttt aacgtcgatg
     9601 cgaaatgggc tgagctggag gccggttatc agtggttaac gcaaaaatat ccgcgtttcc
     9661 ttaacactaa caactataaa cacctgggaa cgctgggaac gggtaaccac tttattgaaa
     9721 tctgccttga tgagtcggag caggtgtgga ttatgctgca ctccggttca cgcggaattg
     9781 gtaacgccat cgggacttac tttatcgatc tggcgcaaaa agagatgcag gaccaacttg
     9841 agacgttgcc gtcgcgtgat ctggcgtact ttatggaagg tacggaatac tttgatgatt
     9901 acctaagagc cgtggcctgg gcgcagcttt tcgccagcct taaccgcgat gcgatgatgg
     9961 aaaacgtggt aacggcattg cagagcatta cgcagaaaac ggtcagacag ccacaaacgc
    10021 tggcgatgga agagatcaac tgtcaccaca actatgtgca aaaagaacag cactttggtg
    10081 aagagatcta cgtgacgcgt aaaggcgcgg tgtttgcgcg tgctggtcaa tatggaatta
    10141 ttccagggtc gatgggagca aaaagcttta tcgtccgtgg gctgggaaat gaagaatctt
    10201 tctgttcgtg cagccacggt gccgggcggg taatgagccg tactaaagcg aaaaaactgt
    10261 tcagcgtgga agatcaaatt cgtgccaccg cgcatgtgga atgccgtaaa gatgccgaag
    10321 tgatcgacga aatcccgatg gcgtataaag atattgatgc ggtgatggcg gcacaaagcg
    10381 atctggtgga agttatctat accctgcgtc aggtggtgtg cgtaaaagga taaatgatga
    10441 aaaggatgat tgcgctggat ggcgcacagg gcgaaggtgg cgggcagatc ctgcgctcgg
    10501 cgctgagcct gtcgatgata accggccagc catttaccat caccagcatt cgtgccgggc
    10561 gggcgaaacc ggggctgttg cgccagcatc tgaccgcggt aaaagcggct gcggaaattt
    10621 gtagggcaac ggtggaaggc gcggagctgg ggtcgcagcg tctggtcttc cggcttggca
    10681 ccgtgcgcgg cggcgagtac cgctttgcta tcggtagtgc cggaagctgt acgctggtgc
    10741 tgcaaacggt gctgcccgcg ctgtggtttg ccgatggacc ttcgcgtgtt gaagtgagcg
    10801 gaggcaccga taacccgtcg gccccgcctg cggattttat ccgccgggtg ctggagccgc
    10861 tgctggcgaa aataggaatt catcagcaaa ccacgctgtt acgtcacggt ttttatcctg
    10921 ccggaggcgg cgtggtggca acggaagtct cgcccgtggc atcgtttaac accttgcaac
    10981 ttggcgagcg cgggaacatt gtgcagatgc gtggagaagt tctattagct ggtgtgccgc
    11041 gccatgttgc tgagcgtgaa atcgctacac tggcggggag tttttccctg catgaacaga
    11101 atattcataa cctgccgcgc gaccaggggc cgggtaatac cgtctcgctg gaagtcgaaa
    11161 gtgaaaatat cactgaacgc tttttcgtcg tcggtgaaaa gcgcgtcagt gccgaggtgg
    11221 tagccgcgca gttagtgaaa gaggtgaaac gctacctggc aagcccggcg gtagtggggg
    11281 aatatctcgc cgaccagctg gtgctaccga tggcgctggc gggcgcggga gaatttacgg
    11341 tcgcccatcc ttcatgccat ctgctgacca atatcgccgt ggtggagcgt ttcttgccgg
    11401 tgcggtttgg tttgatagaa acagatggcg taacgcgggt gagtattgaa tgatgtaaag
    11461 atgtaaaccg gatccaatgc attatccggc taaactcaca ccccatatcc catcatcttc
    11521 agcagtttct gcgcgtgttg taccgcggcc tgacggtggg cgacgccgag tttttggtac
    11581 agattgcgga tatgcgtctt gatggtggtt gccgccactt ccagctcgcc cgcaatttgc
    11641 tcattgctgt aaccggagta gatcagcccc aggacctgcc attcacgttg ggtcaacggg
    11701 ctggtgcgga tcagttctgg cacttccgga tgattcagca gacgttcaac gaaattctca
    11761 tcgaaatggg cgaatttatg ccgatgatgt tgattgattt ctcgcagaat acgctgcgcg
    11821 cgatgctgtt ccagttccgg cagcgtattg agttgaatca gctggcgcag ttgttgcgcc
    11881 atcgcttcgc cttcgatgac aaaatgactg ataaatccgg tgcgattcgc cagttttaat
    11941 gcgtccagca acacgcgctg ggcgtcactt ttacgtccgg cctgccagta cagttgatta
    12001 agcagcaaca ggttacggtt gagatcgctc atcaaccgca gactgcgggc attttcattg
    12061 agttcttcga gaacaatttc cgcaggttca aactcgccca gcaagatttg tgcgcgggca
    12121 atgttgcgcc attgaccttg caggaagtgg ttgttcgcaa actccggttt agccgtatga
    12181 cgcaaccagt tggcagcggc ggctttatcg ccggtcattt gccagtaaat cacccggact
    12241 ttgttggcgt tagagatcca gtcgctgtga tatttgccat tccccagcag gttttccaga
    12301 cggttcagct ggctacgggc gttatctaaa tcaccacggg ccagcgagca ttgaatcaac
    12361 attgccaggc actgaagctg ttgctgtggc tgataagacg acaagacttc aatcccgcta
    12421 cgcgccgacg cttcggcttc atccagccgt gcccaggccc ataacagttg tgctcggatg
    12481 cgcaccagaa actcatgcat cggcagctgt tccagatgct gctcgttgat cagctggaac
    12541 gttttttcct gtgtttccca cgcggtttgc aggaaccctt gggcaaacag aatttcactt
    12601 tgctggatta aactccataa ggcgtagtgc cagacgtcgt gctggcgtgc catctgttcg
    12661 gtttgctgca ttagcgccag tgagcgggtc aattcgcctt tgcagtgcag cacttcaccc
    12721 agcaccgagg ttgctacaat gcggctatag aaccagcccg gtggcagctc ttccagtgcc
    12781 agttttgcca gccgttccgc ttcatccgga ttaccatcgt taatcgccac ctgggcgcgc
    12841 agagcgttaa attctgcgtg catggtgcct tctctgatgt ccttgatttc atgttcagca
    12901 cgggctagca gggtgttaac ttcgccatag cgatgttggc tttgcattag ccacgcctgc
    12961 aataacacta actgcggatt ttccagcaaa ctgtcccacg gcagggcctt aagcgactct
    13021 tccagcagcg acagttcgct atggttgaac aaactccagg cgtgattaag cagaatatcg
    13081 cgcagcatca gcgcatcgcc tgccgccagc gcatgatgaa tcgcttcgct gggaaatccc
    13141 tgggccatcc agctttctgc ggcggcacgg tggatttccg gcagctccgc cgccagttcc
    13201 cactggcagc gctggcgcag gaagttgcca aacagcgggt gatagcagaa ccactcgccg
    13261 gtatcatcca tccgctgtaa aaacagcccc tgacgctcaa tctcttcgag acgcatttgc
    13321 ccgttttctt cgccagtcac acgggtgatg agggcatcgt tcattgagcg caaaatggcg
    13381 cttttcaaca gaaaatggcg cgttgcgaga tcgacgttat ccaaaacctc atcgaccaga
    13441 taatccgaaa gatggctggc attgattccc gccaggcggc gtgccgactt atgggctgag
    13501 tgggtattct gccgggcgga gagggcgatt agctgtagtg ccgtcgccca accggaaacg
    13561 tcatcgcaaa tccaactgct ttctgcggct tcaatcggcg atgacagacg gcaatcaaaa
    13621 aactgcttcg cttcctgatg ggtaaatgcc agttgctgac tgccaatttc cagcagttgg
    13681 tcacgaacac gcagattggc aatgcccagt tgcggaaggt tgcgtgacaa caccaccagg
    13741 gtgagatttt ctggttgatg gcgaataaag aagcgcattg actcgtggat cactggatta
    13801 gtgatcagat gatagtcatc gatgaccaga taaagtgggc tatgccattc cgccagctca
    13861 atgaaaagct gggcgaagag tgacgtcagg ctggcatatt gccgtttttg cgccatcgtc
    13921 tcacatatcg cacagtgacc gttggttgcc tgctgcacgg cggcaatgag atagctggcg
    13981 aaacgctctt gctggttatc accttcatcc agcgagtacc agccgatatc gtttttgcct
    14041 gccgcccact gggaaatgag ggtggtcttt ccgtagcccg caggactcgt gatcagcgcc
    14101 agccggaagt tgttcgcgcc ggaaagttta gccagcaggc gctcacgaac cacggtatgg
    14161 tcgagtcgaa ccggacgact tagttttgac ggaatcagca tagttaatca cttcactgtg
    14221 gaaaatgagg aaatattatt tttttgcgct tcgtaattaa tggttataag gtcggccaga
    14281 aacctttcta atgcaagcga tgacgttttt ttatgtgtct gaatttgcac tgtgtcacaa
    14341 ttccaaatct ttattaacaa ctcacctaaa acgacgctga tccagcgtga atactggttt
    14401 cccttatgtt catcagattc atttaagcaa gggtttcttc ttcattcctg atgaaagtgc
    14461 catctaaaaa gatgatctta ataaatctat taagaatgag atggagcaca ctggatattt
    14521 tacttgtgaa actgtttcac tcctttactt aatttataga gttaccttcc gctttttgaa
    14581 aatacgcaac ggccattttt tgcacttaga tacagatttt ctgcgctgta ttgcattgat
    14641 ttgctgctaa tcctgtggtt tgcactagct ttaagtggtt gagatcacat ttccttgctc
    14701 atccccgcaa ctcctccctg cctaatcccc cgcaggatga ggaaggtcaa catcgagcct
    14761 ggcaaactag cgataacgtt gtgttgaaaa tctaagaaaa gtggaactcc tatgtcacaa
    14821 cctattttta acgataagca atttcaggaa gcgctttcac gtcagtggca gcgttatggc
    14881 ttaaattctg cggctgaaat gactcctcgc cagtggtggc tagcagtgag tgaagcactg
    14941 gccgaaatgc tgcgtgctca gccattcgcc aagccggtgg cgaatcagcg acatgttaac
    15001 tacatctcaa tggagttttt gattggtcgc ctgacgggca acaacctgtt gaatctcggc
    15061 tggtatcagg atgtacagga ttcgttgaag gcttatgaca tcaatctgac ggacctgctg
    15121 gaagaagaga tcgacccggc gctgggtaac ggtggtctgg gacgtctggc ggcgtgcttc
    15181 ctcgactcaa tggcaactgt cggtcagtct gcgacgggtt acggtctgaa ctatcaatat
    15241 ggtttgttcc gccagtcttt tgtcgatggc aaacaggttg aagcgccgga tgactggcat
    15301 cgcagtaact acccgtggtt ccgccacaac gaagcactgg atgtgcaggt agggattggc
    15361 ggtaaagtga cgaaagacgg acgctgggag ccggagttta ccattaccgg tcaagcgtgg
    15421 gatctccccg ttgtcggcta tcgtaatggc gtggcgcagc cgctgcgtct gtggcaggcg
    15481 acgcacgcgc atccgtttga tctgactaaa tttaacgacg gtgatttcct gcgtgccgaa
    15541 cagcagggca tcaatgcgga aaaactgacc aaagttctct atccaaacga caaccatact
    15601 gccggtaaaa agctgcgctt gatgcagcag tacttccagt gtgcctgttc ggtagcggat
    15661 attttgcgtc gccatcatct ggcggggcgt aaactgcacg aactggcgga ttacgaagtt
    15721 attcagctga acgataccca cccaactatc gcgattccag aactgctgcg cgtgctgatc
    15781 gatgagcacc agatgagctg ggatgacgcc tgggccatta ccagcaaaac tttcgcttac
    15841 accaaccata ccctgatgcc agaagcgctg gaacgctggg atgtgaaact ggtgaaaggc
    15901 ttactgccgc gccatatgca gattattaac gaaattaata ctcgctttaa aacgctggtg
    15961 gagaaaacct ggccgggcga tgaaaaagtg tgggccaaac tggcggtggt gcacgataaa
    16021 caagtgcata tggcgaacct gtgtgtggtt ggcggtttcg cggtgaacgg tgttgcggcg
    16081 ctgcactcgg atctggtggt gaaagatctg ttcccggaat atcaccagct atggccgaac
    16141 aaattccata acgtcaccaa cggtattacc ccacgtcgct ggatcaaaca gtgcaaccct
    16201 gcactggcgg ctctgttgga taaatcactg caaaaagagt gggctaacga tctcgatcag
    16261 ctgatcaatc tggaaaaatt cgctgatgat gcgaaattcc gtcagcaata tcgcgagatc
    16321 aagcaggcga ataaagtccg tctggcggag tttgtgaaag ttcgtaccgg tattgagatc
    16381 aatccacagg cgattttcga tattcagatc aaacgtctgc atgagtacaa acgccagcac
    16441 ctgaatctgc tgcatattct ggcgttgtac aaagaaattc gtgaaaaccc gcaggctgat
    16501 cgcgtaccgc gcgtcttcct cttcggcgcg aaagcggcac cgggctacta cctggcgaag
    16561 aatattatct ttgcgatcaa caaagtggct gacgtgatca acaacgatcc actggttggc
    16621 gataagttga aggtggtgtt cctgccggat tattgcgttt cggcggcgga aaaactgatc
    16681 ccggcggcgg atatctccga acaaatttcg actgcaggta aagaagcttc cggtaccggc
    16741 aatatgaaac tggcgctcaa tggtgcgctt actgtcggta cgctggatgg tgcgaacgtt
    16801 gaaatcgccg agaaagtcgg tgaagaaaat atctttattt ttggtcatac cgtggaacaa
    16861 gtgaaggcaa ttctggccaa aggctacgac ccggtgaaat ggcggaagaa agataaggtg
    16921 ctggacgcag tattgaaaga gctggaaagc ggtaaataca gcgacggcga taagcatgcc
    16981 ttcgaccaga tgctgcacag tatcggcaaa caaggcggcg atccgtatct ggtgatggcg
    17041 gatttcgcag cctatgttga ggcacaaaag caggtggatg tgctgtaccg cgaccaggag
    17101 gcctggactc gcgcggcgat cctcaatacc gcccgctgcg gtatgtttag ctcggatcgc
    17161 tctattcgcg attatcaggc tcgtatctgg caggcaaaac gctaaggaag ctcgatggaa
    17221 agcaaacgtc tggataatgc cgcgctggcg gcggggatta gccccaatta catcaatgcc
    17281 cacggtaaac cgcagtcgat tagcgccgaa accaaacggc gtttgcttga cgcgatgcat
    17341 caacgtaccg ccacgaaagt ggcggtaacg ccagtcccga atgtcatggt ttataccagc
    17401 ggcaaaaaaa tgccgatggt ggtggagggc agcggcgaat atagctggct gctgaccacc
    17461 gaagaaggaa cacagtacaa aggccatgta acggggggca aagcgttcaa tctaccgacg
    17521 aagctgccgg aaggttatca cacgctgaca ctcacccagg acgaccagcg cgcgcattgc
    17581 cgggtgattg tcgccccgaa acgctgttac gaaccgcagg cgttgctgaa taaacaaaag
    17641 ctgtggggtg cctgcgttca gctttatacg ctgcgatcgg aaaaaaactg gggtattggg
    17701 gattttggcg atctcaaagc gatgctggtg gatgtggcaa aacgtggcgg gtcgttcatc
    17761 ggcctgaacc cgattcatgc gctctatccg gcaaatccgg agagtgccag cccatacagc
    17821 ccgtcttctc gccgttggct gaatgtgatt tatatcgacg ttaaagccgt cgaagatttc
    17881 catcttagcg aagaggctca ggcctggtgg cagttgccga ccacgcaaca gacgctgcaa
    17941 caagcgcggg atgccgactg ggtcgattac tccacggtta ccgccctaaa aatgacagca
    18001 ttacgaatgg cgtggaaagg tttcgcgcaa cgtgatgatg agcagatggc cgcgtttcgc
    18061 cagtttgttg cagatcaagg cgacagcctg ttctggcagg cagcctttga tgcgctacat
    18121 gcccagcaag tgaaagagga cgaaatgcgc tggggctggc ctgcatggcc agagatgtat
    18181 cagaacgtgg attcaccaga agtgcgtcag ttctgcgaag aacatcgtaa tgacgtcgat
    18241 ttttatctct ggttgcagtg gctggcttac agccagtttg ccgcctgctg ggagataagc
    18301 cagggctatg aaatgccgat tggcttgtat cgtgatctgg cggttggcgt agcggaaggt
    18361 ggggcggaaa cctggtgtga ccgtgaacta tattgcctga aagcgtcggt tggcgcgccg
    18421 ccggatatcc tcggcccgtt ggggcagaac tggggattac cgccaatgga cccgcatatc
    18481 atcaccgcgc gtgcctatga accgtttatc gagctgttgc gtgccaatat gcaaaactgc
    18541 ggcgcattac gaattgacca tgtgatgtcg atgctgcgtt tgtggtggat accgtatggc
    18601 gagacggcag atcagggcgc gtatgttcac tatccggtgg atgatctgct ctcgattctg
    18661 gcactcgaaa gtaaacgtca tcgctgtatg gtgattggtg aagatctcgg taccgtaccg
    18721 gtagagattg tcggtaagct gcgcagcagc ggtgtgtact cctacaaagt gctctatttc
    18781 gaaaacgacc acgagaagac gttccgtgca ccgaaagcgt atccggagca gtcgatggcg
    18841 gttgcggcga cacatgacct gcctacgcta cgcggttact gggagagcgg ggatctaacg
    18901 ctgggcaaaa ccctggggct gtatccggat gaagtggtac tgcgcggtct gtatcaggat
    18961 cgcgaactgg cgaagcaagg gctgctggat gcactgcata aatatggttg tctgccaaaa
    19021 cgtgccgggc ataaggcatc gttgatgtcg atgacgccga cgctgaaccg tggtttgcag
    19081 cgctacattg ccgacagtaa cagtgctctg ttaggactac agccggaaga ctggctggat
    19141 atggccgaac cggtgaatat tcctggcacc agttaccagt ataaaaactg gcgacgcaag
    19201 ctttccgcaa cgcttgagtc gatgtttgcc gatgatggcg tgaacaagtt gctgaaggat
    19261 ttggacagac ggcgcagagc tgtagcgaag aagaagtaga gtgcggttga tgccggatgc
    19321 gatgcatccg gcagtcagtg ttatcaaatc accatattca gcagcagaca gcctaccaga
    19381 ccgcacaccg agataatggt ttccagcatc gaccaggatt tgatggtctc gccgatagtc
    19441 aggttaaagt actctttgaa cagccagaag cccggatcgt tcacatgaga gaaaatcaca
    19501 ctaccggaac ccaccgcaat aaccatcagc tctgggctaa cacccgtcgt tgcaatcagc
    19561 ggtgccgcga taccacccgc agtgattgcc gcaacggttg cggaacccag cgcgatacgc
    19621 agtacggcag caatcgacca ggccatcagc agcggagaaa tgttggtttc gtgcatcatg
    19681 gaagcaatgt atttgtccac gccgctgtct accagcacct gcttgaacgc accgccacca
    19741 ccgatgatca acagcatcat cgcaatgatt ttgatggaag aaaccagcgt gtcgttaatc
    19801 tgatccattg aacgaccacg gttcagacca aaggtgaaca tcgcaatcag cacggcaatc
    19861 agcgttgcca ttaccgggtc accgaggaac tccgctaccg gcaggaaagc gtgacctttc
    19921 ggcaggatca tttcggcaat cgcacgcatc gccatcagca caaccggcac cagagaagtc
    19981 cagacgctga cgccaaagct cggcatctct tcttcgctga aggttttcgc gctgtagaga
    20041 ccttccggga ttggcttatc gatacctttc aacacgcgag cgtaaaccgg accggcgaga
    20101 atcacggtcg ggattgccag aatagtaccg tacagcaggg ttttacccat atcggcattg
    20161 aaaatggtgg caatcgcagt cgggcccgga tgcggtggca ggaagccgtg ggtcacagac
    20221 agtgcagccg ccattggtac accaacatac agcagtggaa tattcgcgga agccgcgatg
    20281 gtaaacacca gcggcagcat cagcacgaag cccacttcat agaacagggc aaaaccaacg
    20341 gtaaaaccgg ttaacaccac cgcccactgg atgtgctttt taccaaattt ggcaatcagc
    20401 gtggtggcga tacgttgtgc gccaccgcag tctgccagca ttttgcccag cattgcgcca
    20461 aaacccatga tcagggcaag gctaccgagc gtcccgccga caccgacttt gatggagtca
    20521 ataactttat ccagcggcat tccttgcatt aatccaacag caagcgccac gaggacgaga
    20581 gcgatgaagc cgttcatttt gaagcggatc atcaggagca acaacaagat tacaccgata
    20641 gcaacaatga ctaatggcat gatttacctg gcctttcatt tgttatgggt aacgtcaatt
    20701 ttctgacgac aaactctaat tatcccaatc gggaacagag atattgcggc accacgactg
    20761 atacccacta aaactaatta ttgtagtcag atgtcaggag tatgtttggt acccatgtga
    20821 atgatacggg taacatctgg cgtttgagaa tcaccagagc ggggtaaatt taaattatga
    20881 gaggttggtc atattatcgc ggggaaacga agggagggat ttgccaaagc aatgctgtgc
    20941 caacgtctgg cacatgttca acgtaggccc gaaatgacgc tttagcgtcg catcgggcaa
    21001 tctacaaaag aggggataac ttagtagtag gagtgttcgc cgcgctggtg ttcggtgaga
    21061 tcgcgcacac ctttcagctc cgggaattcg ttcagcagct gcttctcgat cccttctttc
    21121 agcgtcacat cgaccatgga acaaccgtta cagccgccgc caaattgcag aatggcgtaa
    21181 ccgtcttcgg tgatttccat cagcgaaacg cgaccaccgt gaccagcaag ctgtgggttg
    21241 atctgcgact gcagcatata ctccacgcgc tccatcagcg gtgcatcgtc tgccacttta
    21301 cgcattttgg cgttcggggc tttcagcgtt aactgagaac ccaactggtc ggtaacaaag
    21361 tcgatctctg catcttccag gtatggtgcg cttaactcat caacatacgc ggtcagcagg
    21421 tcaaatttca gggcagtgtc ggtggcttcc acggcatccg gcggacaata agaaacacca
    21481 cattcagcgt taggcgtgcc agggttaatc acaaatacgc ggatttgtgt cccttcttcc
    21541 tgatttgcca gcagtttggc aaagtgcgct tgtgcagcat cggaaatacg gatcatagta
    21601 atggcctaat agttgactat tttagttggt tataatacgc ccatcatcga ggctctacaa
    21661 ggttcgacaa aggcaccaga cctggacagt cgccgcacca ttgcgtaaaa gcaactgcgc
    21721 aatctctgcg acggtacttc cggtggtaac gacatcatcc acaatcacca tatggcgacc
    21781 ttgcacgggc aattcaagac gaaaggcatt tttcaggttg cgcttgcgca gccgggcact
    21841 gagaaaatgc tgggtcgcag tggcccgtgt acgtgtgacg gcttcgctat cccattggca
    21901 gtgcaaccag cgtgataacg gctgacacag caaatcgctc tgattaaatc cccgacgcca
    21961 gtgacgccgc tgccataacg gaacgctgat gatgcgatcc ggcaattgca acccggtggt
    22021 gcgacgagcg tgtaagactt ccaatagtaa cagacgtgac agggcgctgg cgatttcact
    22081 gcgccgggaa aatttaagct ggtggatgag cagacttaac ggcggcgcat agtcggcaac
    22141 cgtgaccagt ctttgccagg gcggcggttt ttgcaggcag cgaccgcagg gaagatggga
    22201 gtgtgtggcg ggtaatccac attgtgggca taacgtttta tctgtgcggg tggcgcgtga
    22261 acagaccgaa caaatccccc aatgacctaa cgccagtggc attcggcata gccagcataa
    22321 tcccggtact gttagcatat gttcatcctt gtaagtcaaa agagaacaat agcggatgaa
    22381 taacatttgg tggcagacca aaggtcaggg gaatgttcat cttgtgctgc tgcacggatg
    22441 gggactgaat gccgaagtgt ggcgttgcat tgacgaggaa cttagctcgc attttacgct
    22501 gcaccttgtt gatctgcccg gcttcgggcg cagccaggga tttggtgcgc tgtcacttgc
    22561 tgatatggcc gaagccgtgc tgcaacaggc acctgataaa gccatttggt taggctggag
    22621 tctgggcggg ctggtggcaa gccagattgc gttaacccat cccgagcgtg ttcaggcgct
    22681 ggtcaccgtg gcgtcgtcac cttgttttag tgctcgtgac gattggccgg ggataaaacc
    22741 ggacgtgctg gcgggatttc agcagcaact cagtgatgat tttcagcgta cagtggagcg
    22801 gttcctggcg ttacaaacca tggggactga aacggcgcgc caggatgcgc gggcgttgaa
    22861 gaaaaccgtt ctggcgttac cgatgccgga ggttgacgtg cttaatggcg ggctggaaat
    22921 cctgaaaacg gttgatctcc gtcagccgct gcaaaacgtg cccatgccgt ttttgcgatt
    22981 gtatggctat ctcgatggtc tggtgccgcg caaagtggtg ccgatgctgg ataaactctg
    23041 gcctcgcagc aaatcatata tcttcgccaa agcggcccat gcgccgttta tttcgcatcc
    23101 ggtcgagttt tgtcacctgc tggtggcgtt gaagcagagg gtgtaggcaa cttttgaaat
    23161 ggcgagacag aaatttactg gctcgccgca gccaactctt cttctgacac cccggtaaag
    23221 cgcatgatgt ccgcaagagg gactccggat tcaagcatta ttttggctat atgcagggct
    23281 ttggattgtt ccccctcctg ccgcaatctt tccgcaattg tcattaaact ctccttgtgt
    23341 ttcggtgaac gttcggcaac gccgtcgata aaatcgttaa aacgtaccgc gtcgccggtt
    23401 tgcagtatgt aattaaacag ccctttgatt tgtctgtcat tagcgtatcc actacttaat
    23461 aagcaggcca tttgctctac cagccccatc aggt
//
LOCUS       DAKISA010000067        23453 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.067, whole genome shotgun sequence.
ACCESSION   DAKISA010000067 DAKISA010000000
VERSION     DAKISA010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23453
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.067"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..156)
                     /gene="cas6e"
                     /locus_tag="OVW45_002673"
     CDS             complement(<1..156)
                     /gene="cas6e"
                     /locus_tag="OVW45_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311637.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HCV5527083.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREEL"
     gene            complement(138..884)
                     /gene="cas5e"
                     /locus_tag="OVW45_002674"
     CDS             complement(138..884)
                     /gene="cas5e"
                     /locus_tag="OVW45_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HCV5527084.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(895..1950)
                     /gene="cas7e"
                     /locus_tag="OVW45_002675"
     CDS             complement(895..1950)
                     /gene="cas7e"
                     /locus_tag="OVW45_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HCV5527085.1"
                     /translation="MTTFIQLHVLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRIAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(1962..2498)
                     /gene="casB"
                     /locus_tag="OVW45_002676"
     CDS             complement(1962..2498)
                     /gene="casB"
                     /locus_tag="OVW45_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HCV5527086.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRAVRLLNGSVNLDSLADGIFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQNQ"
     gene            complement(2495..4057)
                     /gene="casA"
                     /locus_tag="OVW45_002677"
     CDS             complement(2495..4057)
                     /gene="casA"
                     /locus_tag="OVW45_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HCV5527087.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDISAPRADL
                     QGAVWQFLLGLLQTSFAPKDHRRWDDIWEDGLEAEKLREALLSLEHAFQFGPDSPSFI
                     QDFEALTGDKVPVASLLPEIPGAQTTKFNKDHFIKRGVTEYLCPHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWINVMPQDEADLPLPKKFDD
                     LVFPWLGPTRISELAGAVVTDEQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVLLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLNKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLSKWNKEIWLFARQDFDERVFTNPYEPVDLERIMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(4155..6855)
                     /gene="cas3"
                     /locus_tag="OVW45_002678"
                     /pseudo
     CDS             complement(4155..6855)
                     /gene="cas3"
                     /locus_tag="OVW45_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
     gene            complement(7050..7202)
                     /locus_tag="OVW45_002679"
     CDS             complement(7050..7202)
                     /locus_tag="OVW45_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCV5527088.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(7467..8201)
                     /gene="cysH"
                     /locus_tag="OVW45_002680"
     CDS             complement(7467..8201)
                     /gene="cysH"
                     /locus_tag="OVW45_002680"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCV5527089.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(8276..9988)
                     /gene="cysI"
                     /locus_tag="OVW45_002681"
     CDS             complement(8276..9988)
                     /gene="cysI"
                     /locus_tag="OVW45_002681"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCV5527090.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIITGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(9988..11787)
                     /gene="cysJ"
                     /locus_tag="OVW45_002682"
     CDS             complement(9988..11787)
                     /gene="cysJ"
                     /locus_tag="OVW45_002682"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCV5527091.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PQTPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            12106..12468
                     /gene="queD"
                     /locus_tag="OVW45_002683"
     CDS             12106..12468
                     /gene="queD"
                     /locus_tag="OVW45_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCV5527092.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            12546..13817
                     /locus_tag="OVW45_002684"
     CDS             12546..13817
                     /locus_tag="OVW45_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCV5527093.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLEP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            13808..14068
                     /locus_tag="OVW45_002685"
     CDS             13808..14068
                     /locus_tag="OVW45_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCV5527094.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            14085..14660
                     /locus_tag="OVW45_002686"
     CDS             14085..14660
                     /locus_tag="OVW45_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCV5527095.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(14808..15668)
                     /locus_tag="OVW45_002687"
     CDS             complement(14808..15668)
                     /locus_tag="OVW45_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCV5527096.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGESDNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(15665..16444)
                     /locus_tag="OVW45_002688"
     CDS             complement(15665..16444)
                     /locus_tag="OVW45_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCV5527097.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(16422..17831)
                     /locus_tag="OVW45_002689"
     CDS             complement(16422..17831)
                     /locus_tag="OVW45_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCV5527098.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTQLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVDFVVTWLWVPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(17853..19307)
                     /locus_tag="OVW45_002690"
     CDS             complement(17853..19307)
                     /locus_tag="OVW45_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCV5527099.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTDFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(19377..20162)
                     /locus_tag="OVW45_002691"
     CDS             complement(19377..20162)
                     /locus_tag="OVW45_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCV5527100.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVNGGYLVR"
     gene            20482..21759
                     /locus_tag="OVW45_002692"
     CDS             20482..21759
                     /locus_tag="OVW45_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCV5527101.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            21786..23276
                     /locus_tag="OVW45_002693"
     CDS             21786..23276
                     /locus_tag="OVW45_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005137807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCV5527102.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKTWAWSED
                     AAVMEKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTGVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNWGMRIRSRAGRMRE"
BASE COUNT         5630 a         6217 c         5789 g         5817 t
ORIGIN      
        1 gagttcttcc cgacgataaa gaaattgcct ttctttgccg ccagggaaaa gatcccacag
       61 ccactgatgc atcacatatt caccgcgatc caccagatgc agcaactgcg caggcgaaag
      121 ctggccggta tgtaaggtta ttcttgagag gtacatggtt cctccttgct gagccacggc
      181 ccctgattga cggtgcgttc cccaaacagc cactgctgac gatttaaagg aacatcccgg
      241 cggcgtaata ttttgctcgc gaccaggccg tcgtgtttcc cttcccacca gcattcatcc
      301 tgaagtttcg ggagtgagac tttcagttta tggaaatgat cctgatactg ttggtatgca
      361 ttacataagg catcacacgc gttgccttcg agcagtaacg gcgcaagagg taatgccagc
      421 ggatgacttt ttcgccccag ataaagcgga aaaactggat ggcgtaaacc gtcctgcaac
      481 tgttcgaggc tgtaaggcgc atcggctgtt gttgccaccg ccaccatcca ccaggcatcg
      541 gtgtagtagt cgcgccggga gataatcgcg ctcaaaagct cagggtcgct caactcttcg
      601 cgacggctga aataacgtgc tttacgcacc tcttttggca tctggaccgt gtgataatcc
      661 cgtgcccagc gcgggttacg gctggcgcaa accaccagtg aatagtggcg attaaacgcg
      721 tttaagcgtt cggtatcatc acgccgaatc cctaccccgg cagccagtag ccccagcaat
      781 gctgagcgcg aaggaagttc atgggtatga cgaacttcac ctggagcatc gacaccccag
      841 gacgccatcg gcccatgaag ctgaaaaatc agatattggc tcataagccg ccccttacgc
      901 gcagataaag tccagcacgt ccttcatgct tccctgtttg ttcatcacgt caaaacttgc
      961 gcattcggtc ttctgttcgt agaccgtatt catgttttcg cgaagcgttg taatacgctg
     1021 caccgccaca tctaactgac gtgtgccatt aatgggttca tagaaagccg ccgccagaga
     1081 gcgtggttga tcggtgcctt tttctgccag cgcccaggaa gcataggcac ggctggcaaa
     1141 gctattttgt ttaccggttg gggaaacttt aagcgcggct tccgtaaaag cgcgtatggt
     1201 ctgattagct aacgcttcgt caccgccgag gttttctacc agcagatctt tatcaatgca
     1261 gatataggtg tagaaaagtg ctgaaccgaa tccggtttct ccgagatgcc cggcaccggc
     1321 atcttcagag gcctggcgca aatcatcaac ggcggtgaaa aaatcatctt cgacaatcgt
     1381 ttcactgaca ccaaacgcat gcgcgacctg gcaggcggct tcaacattaa actcaggttt
     1441 attcgccagc atacgaccaa acatagcgat atctaccgct atacggtctt tacgtaacaa
     1501 cgcgagatct tcctcttttg gcgcgcgctt ttcttctgcc agttgatgag caagcgcttt
     1561 tacggcgtca aattctgccg ggctgatatg aactaattgt tcagtttcgg cgttagtgag
     1621 cggatctttt ggttttttgt catttttagc tttcccaaga taatcagcaa tttttgccgc
     1681 ccattcgatg gcttttttat cttcaatccc tttctcaatc aggatagttg ccgcctcacg
     1741 cgcaatacgc ccactgcgaa taccaatatg gcccgccagt gcctgttcaa aaagtgcaga
     1801 agtgcgccac gcacgtttca gactttgcga ggaaacgcgc agacgcgttg ctccacccag
     1861 caccacagtt tttggcgcac cggtgtcatc acggttaagg ttggcagcag ggtaagcggt
     1921 taacacatga agctgaataa atgttgtcat gagatagtcc tttattgatt ttgttcgtta
     1981 tcggcgtcgc cagcctggta atattccagc gcccagcgga tacggataaa ttcggttggc
     2041 cgctgctggc ggcggtgatg attgagcagg tcgtcgctct cctggcacca gcggaaaatg
     2101 ccatctgcca gggagtcaag attgacggaa ccgtttaata atctgacggc gcggcgtaac
     2161 tggcgcagta attcatccgg tgtttttact gctgacaggc gagtaaaacg cccctttgac
     2221 attaccgccg ccagttgcgc ggcaaaaggc tgccgttcat caatggcttt aacattggcg
     2281 ctaactgcgg ctatcagcgc cagtgcggtg atacgccact caggttcatc ctgccacttt
     2341 atttgtctgt tcttcagaaa caggcgaaat ccatccgtca gacaaacatc attaaccgtc
     2401 gtactacgcc gcagactggc acgttcgccg cgtttctcct gcaattcctc gtgccatttg
     2461 cgcagcgtgg ctttgtgctc ctcttttaca atactcattc agcagcctcc tgctttttct
     2521 ccctggcggc tttagcactt tgcttctccg ccgatgttgt aaaatatttc ttgcgcgcgg
     2581 tcatgatgcg ttccaaatca acgggctcat aaggattggt gaatacacgc tcgtcaaaat
     2641 cctgacgcgc gaataaccaa atttccttat tccatttgct gagtaattca tccgcatcct
     2701 gaccttcttc aatttggcgc actaacctca ggaagcgatg ctgagttttg ttccagaagt
     2761 cgatatccac aaaactaaaa tcaccccttg caccttttgg atcggagaac catgcttctt
     2821 tcaatgcact ccgtaacaga cttagaatcc gtgaagccgt ttgcgcagcc agccgcagtt
     2881 tcggtatctg gccttctttt ttattgagca gcagcgggaa atgatgttcg taccaacagc
     2941 gcgctttcat gttgtcgaaa tcatagccaa atccccacag gcccactttt gcctgcttga
     3001 gactgctggc attaaagagt ttcaccacca gcgcgggaag ttccgtattg ttttctgact
     3061 tacccgtttc gataaggcct aaccagtcgc gccagattaa accgcccggt tgtggtttga
     3121 ccgagtaaaa ctcgccgccc tctttaagta gtacacggta aggcgttaag ggatgctgcc
     3181 acatggcata gttagcgccg taatttttgg tcgtcatcag gctcagaagc gcgtcgctct
     3241 gctcaccgca aatatcgcag ttgccgactg tggtggtatt aaaatcaata cgaatacggc
     3301 gaggcattcc ccagtacgcc tggagtttat tgacctgctc atcggttacc accgcaccgg
     3361 ccagttcgct gatgcgcgtc gggccaagcc aggggaaaac cagatcgtca aattttttgg
     3421 gtagcggtaa atcggcttca tcctgcggca tgacattgat ccacagtttg cgccacaagg
     3481 gagtttgttg attaccctga tattcctgta attcaatcag agtggtcatc ggcccaccgc
     3541 cgcgtaaacc ggtgcggtag cctttgccac ctgacggcgc attcaactgt agggagaaca
     3601 gagctaacgc agagcaatgt gggcacaggt attccgttac accacgcttg ataaagtggt
     3661 ctttattaaa cttcgttgtt tgagcgccgg gaatctcagg cagtagcgaa gcgaccggaa
     3721 ctttatctcc ggtgagcgcc tcgaaatcct gtataaatga aggggaatct ggcccaaact
     3781 ggaaagcatg ttctaatgac agcaatgctt cccgtagctt ttcagcttcc agtccgtctt
     3841 cccagatatc atcccaacga cgatgatctt ttggcgcgaa gctggtttgt agtagcccca
     3901 gcaaaaactg ccataccgca ccctggagat ctgcccgcgg cgcagaaata tcgacaacat
     3961 tttcatccgc cagatcgact ggcgccagtt tgcctgtcgt tccgtcttta aaacgaacgg
     4021 gcaaccacgg ggttgtcaga agtgaaaacg agttcatacg catcctctcg taaaaaagac
     4081 catcccaggt cggttgtcca tctttccatc cttttcggtg tatcaactat tcagagagtc
     4141 ctgtcgcaga catctcaacc aaccttgcca atcaatcctt ccgtagccga atacccgcaa
     4201 ctttcgtcat cgttgactaa aataacgttt gccatctccg gatcttgtcg ctgttcaatg
     4261 caccacagcc tgaacgcctc tccctccagt aaagaaaact catcccgatg ttttttccac
     4321 cagcttcggc gcactctgac aacgctcatt tcccatgcgt gagcaccggt ggcataaggc
     4381 ttcaccacac cgtcaataca ggtagccagc cacagggaaa cagactcctc agccagacgt
     4441 gtcgacagtt tttctgggag gtaatcgttg atattggcgg catagccggg cctgaagttc
     4501 agcacaaact ttttagccat tgcgcgatca cagtaatatt tgcccacttg ctcctgctcg
     4561 ctgcgggcaa atccttccgg cattaccacg tcctcaccgt agacggattc aataagaagc
     4621 cgggctgcgt gtggcatctg aatagcacct tgctcacgca gcacacgctg cgtcagccag
     4681 attcgcccat gatcgggata gacaaatgca ctgttacgca tggcactgcc aaaccattcg
     4741 tcgccaggag agtcgtccca gacgggagcc agaatcagca attcaggggg gggaacgctc
     4801 gtcctttccg tcacgcttta actgaccatt aatatcgcgg atatgccgct gtaatcgtcc
     4861 tgctcgctga atcagcaaat caacaggggc caagtcggag atcatttcgt ccaggtcaca
     4921 atcgacactc tgctctaata cctgagtaca aataaggact tttccggcac gctgtgagct
     4981 atcttcttta ccaaagcgtg ccagtgtctc cgtttcaatt cgctggcgat cgctaaaagc
     5041 aaagcggcta tgaaaaagtg aaaggctgga agcaggaatg acgccgcggg caagcagctg
     5101 acgatgaacc ttaatagcgt catcgacaga attccggatc caggcgatgc atttcccctg
     5161 acttaccgcc gattcgatac gcgcaataca ctcttgttca ctatgaagcc aacccacgct
     5221 gacgctacgc tcaacgtctt tgcgcgtcgc tacccggtgt gagttcacat cggatttcgt
     5281 gacatgcgtc agccaggggt aatcatcctt ttccaggaac ggagcttctt gcaggccctc
     5341 tgtaccccgc gcaaaggcag cgacgagttt gtcgcgctgc tgttgagata acgtggcaga
     5401 aagcaaaata acgctgtttc cgccacgcgc ctgccgctcg atcaaccctt caagaatgca
     5461 cgacatgtaa gcgtcacagg cgtggatctc atcagccagc aggattttgt tactcagccc
     5521 cagaagccgc agattattat gtttgaacgg catcaccgcc atcatcgcct gatccagcgt
     5581 gccgacgcca atttcagcca gcaacgcctt cttgttactg tcggcaaacc aggccgcaca
     5641 tccctgactg aacgtttgtt catccggttc ttctgacccg actaaatcac cagaccagag
     5701 tgattcatta aagcggtcca ttaatgtgcg ggcactatgt gccagcacca ggctggggcg
     5761 ggattctggc gaatagaaag caagccaggt tttgaccagc cgatcgtaca tggcattggc
     5821 cgttgccatt gttggcagac caaaaaacag cccctgagcc ttccccgcag ctatcaacct
     5881 gtgcgccagg ataagcgccg cttctgtttt acctgcgcca gtcacatctt ccagaataaa
     5941 taactgcggt ccttgctggc tgatatccag atccagcacc ttttgctgta acggtgtcgg
     6001 attctcaata aaaggaaaca gcgtattaat tccgttgaaa ggcgcgactt ttgcttttag
     6061 aggaaagacg gttaaggcgt tttgagcctg aactaaagtt ttctgccagt aacctttaat
     6121 atccattggg tgtgcgacgc gtggaaaaaa tcgcgttgac gaacccgtcc agtctgcgag
     6181 cacgacggtt gcagagatat accaggaaag gtgttttata agttcaatgc cctcatcatc
     6241 atcccagaat gcggggatct ctatgagcgg aaacagtggt ttgatttcaa ggagaaaatc
     6301 tcgtgctgca gctttgtctt caggcagaaa attatccaat tcatccatac gatcaggggg
     6361 ccgaccatga tgccctgtcg tcacgggcat ccacatttct atcacttgcc taagtttgcg
     6421 cggagagagc gaagatgaag gaagtaactc ctgacattca cttaaataat gattccacaa
     6481 ccagtagccc agcgttgaat gagagatttt ttcgtaattt tttctggcac cttcctgaat
     6541 cttgagttca gggggcaggt aaagttgttg aaaagagcgg gcaaattttc caatatcgtg
     6601 ccagcacagc aaccaggcga aaaattgagc cgcctgttcc ttgtcagaaa tccctaattg
     6661 acgaaaataa tcagccagcc cgaagcaatt tcttttaacc attaaatagc ccatcgcggc
     6721 tacatccagc gaatgccagc aaagaaggtg atagtcgtcg cccccgtctt tctcgccgcg
     6781 tcgggttttt ccccagaaat caaaaaaagt cacaatattt ttatccttca gtaaacttaa
     6841 aggatattta cgcatatatt aaaaattatc tgtgatatat atcattcaat aaaatattta
     6901 cattatgaat aatcaaataa ttttcatccg taatatatat gaataataaa cataggcata
     6961 atattcacat aaataatata aattaaaaaa gggtgcagga gaccaaacca tcacaattca
     7021 gtacgcgggg ttttaccccc gcgtggtggc tacttcttcg attcgtaagc gagaactgcc
     7081 ttaaactcca taccacgttc tctgatactc aactcacaca acgagtcgcc taccatcagc
     7141 gtgaatccca gtactgtaaa acacagcact atgattgcca caagggcata ttttgtcagc
     7201 atgtcttgcc tccctgcaaa gaagagacta acattcgaat tgtttgggtt cgaacgctgg
     7261 cctcaggttg atagaaatat cgcctggggc ttttgtccat ctggaacctc gcgaatgctt
     7321 aacgccagac agcctcaagc acccgacgcc attctatacc tgataattct tccagcggcg
     7381 ttcacgttta aaatcaggaa tactgctcgc aaaataaaaa cgcccccatc atttctgaca
     7441 gaggcgttta atttatccgg caatatttac ccttcgtgca atccacattc cctttttaag
     7501 ccaaagaaac gtgtttcttc ttccgacatg ccaggttccc atttacgggt tgtatgggta
     7561 tcaccgaccg ataaatatcc ttcatcccat aatgggtgat atttcaggcc atgtttttgc
     7621 aggtactgat aaatagttcg gttatcccag tcgataatcg gcagcacttt aaatacgcca
     7681 cgctgaattg ccagcaccgg taaattggcg cgactgccgg attgttcacg gcgcaggcca
     7741 gcaaaccagg tttgcgcgtt aagttctttc agagcccggt tcatcggttc gactttgttg
     7801 atgtcattgt acttttcaat gccttcaacg ccctgctccc acagttttcc gtagcgtgct
     7861 tcctgccagg ctgcgctttc ggtagcacgg tacactttca ggttgagctt gagtttgtcc
     7921 gttaactcgt caataaagcg gtaggtttcc gggaacaagt aacccgtatc ggtgaggatc
     7981 accggaatat ccgggcgaat ttgattcacc agatgcaggc tcaccgccgc ctgaatgcca
     8041 aagctggaag aaagtacata ttcaccgggc agattatcca gcgcccaggc tacgcggcct
     8101 tcagcgtcca gtttttccag ttcggcgtta gtttccgcca gcgccagaat acgatctacc
     8161 ttcggcagtt cgttcagggc gtttagatcg agtttggaca tagattcctc actgtttgcc
     8221 ttgcctgatg cgacgctcac gcgttcttat caggcctaca agaccgggct gatggttaat
     8281 cccacaaatc gcgcgccgga tcgagcaccg ggcgaatgat gcccgcacgc accgtaaagt
     8341 cgccgaagcc ttcacccgct tcgcgctctt tcgcccagcg ccctatcagt tcatcaagcg
     8401 acgccaggat ttccggctcg gtgatgtttt ctttatacat ccgtgggata cgtgtcccaa
     8461 tgcggttgcc gccaagatgc aggttgtagc gacccggcgc tttacccacc aggcccactt
     8521 ccgccagcat cgcgcgacca caaccgttcg ggcagcctgt tacacgcatc acgatatgct
     8581 catcgctgac accatgtttc gccattaaat tatcgatgtt gtcgataaaa gacggcagga
     8641 aacgctctgc ttccgccatc gccagcgggc aagtcgggaa tgacacgcag gccatcgagt
     8701 tttcacgctg cggcgtgacg gcattcatta acccgctctc tttggcgatc ttctcgatct
     8761 tcgctttctc gctttccggt acaccggtga tgatcagatt ctggttcgcc gtaatgcgga
     8821 aatcgccttt gtggatcttc gcgatctcca gcaggccggt tttcagcgga cgccccggat
     8881 aatcaaggat gcgaccattt tcgataaaca gcgtcaggtg ccagttatca tcaatgccct
     8941 taacccagcc aatacgatcg cctcgtccgg tgaactcata tggacggatc ggttcaaatt
     9001 tgatccccgc gcgacgctcc acttccgctt taaacgtctc aacccccacg cgctccagcg
     9061 tgtatttggt tttggcattt ttacgatcgg ttcggttacc ccagtcacgc tgagttgtca
     9121 cgacggcttc ggccaccgcc agcgtatgct ccagcggcag atagccaaac tcgctcgccg
     9181 tgcgggcgta ggttttcttg ttgccgtgtt cgatagaaag cccaccgccc accagcaggt
     9241 tgaagcctac cagcttgccg ttttcggcga ttgccacgaa gttcatgtcg ttggcatgca
     9301 gatcgatatc gttctgcggc gggatcacta ccgtggtttt gaacttacgc ggcaggtagg
     9361 tctgaccaag gatcggttct tcatcagtag tggcgacttt ttcctggtcg agccagatct
     9421 ccgcatacgc gcgggtacga ggcaacagat gctcagaaat cttcttcgcc cactcgtacg
     9481 cttccgcgtg cagctgcgac tcgtaagggt tcgaggtgca gagtacgtta cggttcatgt
     9541 cgttagctgt cgccagcgca tcaagaccga ccgagtgcag catctggtgc accggtttga
     9601 cgttcttttt cagaatgccg tggaactgaa acgtctggcg gttggtcagg cgaatgctgc
     9661 catagatggt gttttcaccg gcaaatttgt cgatcgcctg ccactgttta gtggtaatca
     9721 ccccacccgg cagacgacag cgaagcagca tcgcgtggcg cggctccagc ttctgttcag
     9781 cacgttcggc acggatgtcg cggtcatcct gctgatacat gccgtggaag cggatcagca
     9841 ggaagttgtc gcccttaaag ccgccggtca gaccgtcgtt taaatcttcc gcaatggtgc
     9901 cgcgcaggta gttgctttca agcttcatgc gctcggcgtc tgtcagtttt ccttcgacca
     9961 ctaaaggccc tggatgtttt tcgctcatta gtagacatct cgctgataac ggcgctctac
    10021 gcgcagctca cttaaaaatt catccgccgc ttcggtgtcc atgccaccaa attcggcaat
    10081 cacttccaga agtgcctgct caacgtcttt cgccatgcga ttagcgtcgc cgcagacata
    10141 aatgtgggca ccgtcattga tccagcgcca cagctccgcg ccctgttcgc gcagtttgtc
    10201 ttgtacataa actttttctt tttggtcgcg cgaccaggca agatcgatac gcgtcagcac
    10261 gccctctttg acgtagcgct gccactccac ctggtagagg aaatcttccg taaagtgcgg
    10321 gttgccaaag aacagccagt ttttacccgg cgcttcgtcg gcggcgcgtt gctgcataaa
    10381 ggcacggaac ggcgcgatgc cggtaccggg gccaatcata atcactggcg tttgcgggtt
    10441 agcgggcagg cggaaattgt cgttatgttc gataaatacg cggacttcgc cctcttcttc
    10501 cacgcgatcc gcgaggaagc tggaggcacc accggcacgg gcgcggcctt ccacgtcgta
    10561 acgcaccaca ccaacggtga cgtgtacttc gttctcgact tccgcctgcg aggaggcgat
    10621 ggaatacagg cgcggggtca gcgggcgcag caggttaatc agcgcttcgg catccagttg
    10681 cgccggagag aaacgcacca tgtcgacaat cggcgtggtc gcggcgtaat gctgtaactt
    10741 cgctttatcg cccaccagcg gcagcagcgt ttcgctgcgg gtaagcgtgg catagttctc
    10801 aacaatgttg gcggtgttga cggtcagttc gaagtgccac tgtagcgctt cgttcagagg
    10861 caacgttttg ccctcgacgg tgacaggttc atcgcctttc agccacagca gttcgacaag
    10921 ttctttcacc agtgccgggt cgttctgata ccagacaccc agcgcgtcac ccggctggta
    10981 acgcaggcct gaatcgccta agtcaatttc gatatgacga acgtcttttt cagagttacg
    11041 cccggtaatt ttctggttaa ccgaaaggct cgccaccagc ggcgcgtctt tgctgtacgg
    11101 gctggtgtgg atttcattta ccgcgccagt agcgacggat tgcgaaggtg ccgcgacagg
    11161 cgcacgcgat ttaagcgcat caaccacgcg ggcgcgccac tcgctggcag cagcctggta
    11221 ttcaacatcg gcatcgacac ggtcgagcag gcgttcacca cccagttccg ccagcttgct
    11281 gtcgaaatct ttcccggact ggcagaaaaa ttcataagag ctatcgccga ggctaaacac
    11341 ggcaaacgcg gtgttttcca gctttggcgc ttttttggag aacaggaact tatgcagcgc
    11401 gacggcttct tccggcggtt ccccttcccc ttgcgttgac gtcactacga tgagcagttt
    11461 ttcgctggcg atttgtttga atttatagtc gcccgcgttc accagcttaa cgttcagttt
    11521 tgctgctaat aaatcatcgc gtaatgcttc agcaacccgg cgcgcattgc cggtttgcga
    11581 ggcggagata atagttatac ccggcatttc tgcggctggc gctggcgtcg ctgcaagcgc
    11641 agcaggctgc tgattgagta cgccccagaa atagccagaa acccaggcaa gctgggtagg
    11701 cgttaaatcg gtcgtggccg cctgaaggcg tgccagttgc tccgggttca acggaagcaa
    11761 cgcggaaggt gggacctgtg tcgtcatgcg tcgttatgtt ccagtaagca aagctgtttc
    11821 tgcgccctgt cagcgcccat aaaacagaag agaagataag gttaacgggg tgaacggtgt
    11881 ggattaaaga cgggatagcg ataactaata accaaatcga ctaacctgtt ttagcaatag
    11941 tctttaacaa caaaatagat taaccaaccc aatgaaaaac aaatgaattt agccaatcat
    12001 taagataaat cagcgatttt gcgcaacaaa gtcgttttag ataatgcgaa aaaacagcct
    12061 ttccggtact ctacggcggt tttatcgtcc tgtagagaaa ttatgatgtc caccacgtta
    12121 tttaaagatt tcaccttcga agccgctcac cgcttaccac acgtcccgga agggcataaa
    12181 tgtggtcgcc tgcacgggca ttcctttatg gtgcgactgg aaattaccgg ggaagtcgat
    12241 ccgcatacgg gctggattat cgatttcgct gaactgaaag cggcgtttaa gccaacttat
    12301 gagcgcctcg atcaccatta tctcaatgat attccaggtc tggaaaaccc aaccagcgaa
    12361 gttttagcaa aatggatttg ggatcaggtt aaacccgttg tgccgctgtt aagtgcggtg
    12421 atggtaaaag aaacctgcac cgcagggtgt atctatcgcg gcgaatgata agagtgtgtc
    12481 ggcggtcaat ttcccttaag taacgctatg ttagggtgtt gtgttctgga tatctggggc
    12541 atgacatgga agacgactgc gacattatta ttattggtgc cggtattgca ggcaccgctt
    12601 gcgcgttacg ctgcgcgcga gcgggtttat ccgttttgtt actggaacgc gctgaaatcc
    12661 ccggcagcaa aaatctttcc ggcgggcggt tatataccca tgcactcgcg gaactcctcc
    12721 cgcaatttca tctgaccgcg cctcttgaac gacgcatcac tcacgaaagc ctttccctgt
    12781 taacgcccga tggcgtaacg acgttttcca gcttacagcc cggcggtgaa tcctggagtg
    12841 tattacgtgc acggttcgat ccgtggctgg ttgccgaagc cgaaaaagaa ggtgtcgaat
    12901 gcatccccgg agcgacggtg gatgcactgt atgaagaaaa cggcagagtc tgtggcgtta
    12961 tttgtggtga cgatattctc cgcgcccgtt atgtggtgct ggcagaaggt gccaacagcg
    13021 tcctggctga acgtcacggg ttagtgactc gtcctgctgg cgaagcgatg gcgttgggga
    13081 tcaaagaagt gctgtcgctg gaaacatccg ctattgaaga acgttttcat ctggagaata
    13141 acgaaggcgc agcgttgctg ttcagcggcg gaatctgtga tgacttaccc ggcggcgcat
    13201 ttctttatac taatcaacaa acgctctcgt tagggattgt ttgcccgctc tcttccctta
    13261 cgcaaagtcg tgttccggca agcgagctgc tgactcgctt taaagcgcat ccggcagtgc
    13321 gcccgcttat caaaaacacg gaatcactgg agtatggtgc gcatctggag ccagaaggtg
    13381 gcttgcacag tatgccggtg caatacgccg gtaacggctg gctgctggtg ggcgatgcgt
    13441 tgcgcagttg cgtcaatacc ggaatttccg tgcgcggcat ggatatggcg ctgactggcg
    13501 cacaggcggc ggcacaaacg ctaataagcg cctgccagca ccgcgagccg caaaatctgt
    13561 ttgcgcttta tcatcacaac gtagagcgca gcctgctatg ggatgttcta caacgttatc
    13621 agcatgttcc ggcgcttttg caacgccctg gctggtatcg ggcgtggcct gcgttaatgc
    13681 aggatatttc ccgcgattta tgggatcagg gtgataaacc tgttccaccg ctgcgccagt
    13741 tattctggcg tcatttacgt cgtcatggcc tgtggcatct ggcgggcgat gttatcagga
    13801 gtctgcgatg tctgtagccc ataatctctg gcgcgttgct gatgcgccgc acattgttcc
    13861 ggctgactcc gttgagcgtc agatggtaca acggttgatt aacgcctgtc cggcaggtct
    13921 tttttcgctc acaccggaag gtgacttacg cgttgattat cacggctgcc tggagtgtgg
    13981 cacctgccgt ttgctgtgcg acgaatccac actacaacag tggcgctatc cgccttccgg
    14041 attcggcatc acctaccgct ttggataaat aataaggata atttatgccc ctcttacacc
    14101 tactccgcca gaatccggtg attgctgccg ttaaagataa tgccagcctg caactggcta
    14161 tcgattctga atgccagttt atttccgtgt tgtacggcaa tatctgcacc atcagtaata
    14221 tcgttaaaaa gattaagaac gccgggaaat atgcttttat tcatgttgat ttgctggaag
    14281 gcgcgtcaaa taaagaagtc gttattcagt ttttgaaact ggtgaccgag gcggacggca
    14341 ttatcagtac caaagcctca atgttgaaag cggcaagagc ggaaggtttt ttctgtattc
    14401 atcgcctgtt tattgttgat tcaatttcgt ttcacaacat tgataagcaa gttgcgcaat
    14461 cgaatccgga ttgtattgag atcctgccag gctgtatgcc caaagtgctg ggctgggtga
    14521 cagagaaaat ccgccaaccg ctgattgccg gtgggctggt gtgcgatgaa gaagatgcgc
    14581 gtaatgcgat taacgcgggt gtcgtggcgc tttccaccac gaataccggg gtctggacgt
    14641 tagcgaaaaa attactttga cgggataatc gtaaccaatt gaatttggtt tgatttgtag
    14701 gccgcacgcc acatccgaca ttcagcgcct gatgcgacgc ttgacgcgtc ttatcaggcc
    14761 tacaagtccc caggccagaa ccgtaggcag gatcatccgg caacacgtta ttggcaatct
    14821 gcgtgaatgt ttgtcaccag tgcctccagc accactttcc agtcatcaac cacgccaaca
    14881 tctgcctgtg aaaataccgc agcgctggcg tcatgattaa tcgccaccac aaacttgcta
    14941 ttgcgtacgc ccgccattaa cgctgctgcg ccagaagcgc caacaacaat acacacctca
    15001 ggtgccagca gatgtccgga aataccaatc actttttcag catcaacgcc accgttcatc
    15061 actcgcgccc gactgtagcc cacttcggct cccagctttt ctgccagcat agcgatttcc
    15121 tggttatctg actctccgcc ctgccccacc accagcaccc gtctggcctc agctaatggg
    15181 tcgcgagtaa catttttcag atcctctgtg ctaaccagcc agtccggaag cgcacccggg
    15241 acaatgttca gttgttgcat tccagagggc aacgtggcgt ttttcgctgc tcccgcctga
    15301 cgcgccaggg aaaggcatag cgggcgtttt tctgtttgca gcgtcgccgt tagcgcattt
    15361 ccccagtggg atttgcgcac gcttacggtt ggaatatcta acgaagtcac ctggcagatg
    15421 cttgcaccat gtaaacgcca ggccagccgg gtcgacagtt catcgccaaa cgttcccggc
    15481 ggaaagagaa caacgtccgc aggcgttcgc tgccactgtt ccaccaacgc atccagcacc
    15541 tgttccgcca ctacaggctg aggttcaatc tgccagtgcg ccagggtaca accgctgaaa
    15601 tcctgcgccg ccagccagct tgctattgcg gcattctcct gatttattgt cacaattgcg
    15661 atattcatgg ctgcatcctt tggcgtaaat agtcctgcca tagcttttga gctttttctg
    15721 ctaccgtttg cccatcaatc agcgtcgctc cgcgtcgttg ctctgctctt gccagttgca
    15781 gacactgcat cgctggcatt tcggcagcaa cagttttacg gataatttct gctttccccg
    15841 cagccattcg ctggcgcatt cccggtactg gcaaggccac ttcaccgcac tgacgcacgg
    15901 caatcaccgc aggcaagcga acccggcaac aacgcaaccc atgttcagta cgctgttcga
    15961 gggtgataaa cagcgcgtcg agagtgaaac gctccacctg ggtgaagcag ggccagccca
    16021 gcatttccgc cagcaaaaat ggcgtttgcc cattctgccc ttcgctgctt tggcagccgg
    16081 taattatcag atccagcgga ttctgatgct gccattcggc aatatggcga gcgacaaatt
    16141 ccggcgcaaa gcgcagatct gccgccgtct ccagcaatac agcttcctca aacccaagag
    16201 ccatgagata gcgcagccag tgcaatgccc gttcatcccc catgctcaac gcggttaaag
    16261 acatcggcgt accgtttttt ctctgcgcca acagcagcgc ggcagcggcc tgttcatcag
    16321 caccgagtaa acttcgcagt agcgaaatat ccggtccgct tttaccctga gccgccgcct
    16381 gccactcttt ttccgccagc attccggcat ccggttcggc tttaaacgct aacagaatgt
    16441 tcattcgcac ctcctacatt tcccgccgcc accagcggga gggctttagt ttctggtacc
    16501 catagccagg tcacaacaaa atcaaccaac aacactgtcg ccaggagcaa taaggtgact
    16561 tgcattcccc actgcgccag cacccacggc agcaggccag tacttaccgc cgcgccaagt
    16621 cgactcatgg cggtggcaaa accgacgccc agcgagcgaa tgtcagtagg aaaactttcc
    16681 gcaggcaaaa tgcccaccag attactgact gccgaaatgg tggtgctgaa gagaacaaaa
    16741 agcagctgcg ttaatgaact gccggaaggc aaacaggcca tcactaccag cgttgccgcc
    16801 agcagcaaaa aacttcccag caaaaatttg cgatgtgcca gcaggtgcgt cagaactaat
    16861 cccagcagcg cgcccacaat taacaacgca ttaagcatca ggctggcagt cagcgcatct
    16921 tccagaccaa tagtctgggc gatagttggc agccaggtat aaatcacaaa ccatgggatt
    16981 acgaggcaga caaagaagac gctgttaaac gccgtgcgcc gccagtaacg cgaagagaac
    17041 aaggttttga tgtgtttatg ggtcgccgtt accacttcat cgcccagtaa aacatgggga
    17101 ccaaaatagc gatgcacgat agcgtgagct tctgcaaaac gcccctggcg cagtagccag
    17161 cgtggtgatt ctggcgttcc ccagcgtaat aacgtaatca acaacgcggg cagagctgcc
    17221 gatgccagta gccagcgcca ggcctccggg ttttcggaaa taaagtgatg tccggcaata
    17281 cttgccagca catagcctac ggtccacacc acgctgaatg cgcccagcaa aataccgcga
    17341 tggcggcgcg gggaaaattc agccagcaag gtgtgaccta ctgaataatc gcctcccaga
    17401 ccaatgccaa tcaaaatgcg cagtccaata agatgctctg gcgtggtggc aaaaaattgt
    17461 aaaaacgaag caagcgtaat cagcaaaaag ctgaaggtga agattttttg ccgaccaata
    17521 tggtcggaga tccacccaag aaccaggctg ccaaggaaca aaccaaggag cgccgagccg
    17581 ccgatcattc ccgccataaa cggcgtcagt tgcatggcgg gcgtaagctg aataatggcg
    17641 taaccaatga cgccgagaac ataaccgtcg gtcaggtgtg cgccgaaagt gagcgcagca
    17701 atgcggcagt gaaaacggtt aagcggtaaa tcatccattc gcaccggtga agtgttcatc
    17761 tttacccctt cattgtccgg taataagaac ggcttctcgc ctgagaagcc gaaaaggcaa
    17821 cgccccgtca tcgcggaacg ctgcctgaca cattattttt caatcggata gatagtaccc
    17881 gtattcataa tgccattagg atcgaactgc tttttcagcc cttccagcaa cgcccacgcg
    17941 ctgccgtgtt ccagtttgct ccagtgaacg cgatgtttac cgataccgtg gtggtgcacc
    18001 atcgaaccgc cgaggcgaat ggtttcttca cagatgatct tgttgagcgg attgtggtac
    18061 ttgtcgattt cctcttccgg cttacagtca acgacgttgt aatcgtagac gaagtacatg
    18121 ttggtgccgt tctgatagct atgagaggaa tgaccgccca gcatggtgat gtcgtcggcg
    18181 tgcgggaact cagtacgaat acggttaata acgctttcgt agatttcgtg gatgcagctc
    18241 cagcagccgg acacttcagt ggtaaagccc atgttgccgg ttttgaggat ctgcacacgt
    18301 tcggcagcca ctttatccgg tccccagttc aggttgttaa accaggtttc gatcagcttg
    18361 ctgtccacgc gctggcattg cgggtagcgg gcaacgattt ccgcaatccc ttcgcccgtc
    18421 gccttcgcaa tgcgagggtt accttcagcc ataaagatca gcacgcattt tccgtctgca
    18481 aaatgggtga agtgttgggt gccatcttca gcgtcataca aacgagcgat cgacggacga
    18541 tacccttcca ccatgacttc acgcaggatg ttgaagtcgg ttttcatgtc ttccaggata
    18601 tagccgtaga agaggttgtt ttccggggtg aatttaaaga ttttcactgt tacttcagtg
    18661 atatagcaaa gtgcaccttc gttaccgatg atgatgtggc gaatgtccgg gccagccgcg
    18721 cggcgtggca cgtttttaat gcgtgtgacg gtgccatcag ccaatactgc ttccagacca
    18781 acgaccatat cttcgattgc gccgtagagt gtggagaact gcccgatact gcgggttgct
    18841 accaagccgc ccatctgcgc cagcggcttt gactgcggag aatgccccgt ggtgtaacct
    18901 ttttcacgca acgcattttc cagcacctcc agcggaacgc cacattgcgc cgtcgcctgc
    18961 atattctcaa tatcaatatt aatgatttga ttcatggcgg agccgtcaag caccaccgag
    19021 ttttccacaa cggtttccag cccaccttca gtggcggaag cgccggtacg cggcacaccg
    19081 ttaattttgt gcgcattcat aaaattcagc acacgggata cttgctctgt ggaaccgagt
    19141 tttacgaccg ctgccggaat cggcaaagta taaatgccat gaatatccgg aaatttacga
    19201 aaacggtcaa tactgttctt ctttaatact gtttcatcgg taattacgcg atctgcacca
    19261 acaatttcct ttagttggtc gacaatcgct gcgcgagata aagacatagt aattccttcc
    19321 tgataataaa aatggaataa tcattaataa gagaaaacag aaaccaccgt tatggattag
    19381 cgcactaaat aaccaccatt gaccactaat aaatgcccat tgacataatt tgaagccgga
    19441 cttgcgagga atacagctgc gcccattaaa tcctgagtat cgccccaacg gtttgccgga
    19501 atatgatcaa gaacgcgctg attggtttct ggattactgc gtgtcgccag cgtaatatct
    19561 gttgcataat agccaggagc aataccattt acctgaatat tatattgacc tagttcatca
    19621 caataagctt tggtgaatcc ggcaagagca tgtttagtgg cagaatatgc aggtgaccat
    19681 tgtccaccta agtaagagaa caatgaacag atattaataa ttttgccgct tttctgcggg
    19741 atcataattt ttgcagcttc atagcttaac tcgaatgcgg cggtcaggtt cacatcaatc
    19801 atcggatccc agtcggcacg accgaagtcc agcaccttat tcagcttaca aataccggca
    19861 ttgtttacca gaatatcaac tgtaccgaaa cgctcacagc aagcagcgat aatcttctgc
    19921 ggcgcgcctt ctgcggtgat atccacctgc atgaagtcca cctcaacacc ctgtttttca
    19981 atcatttcct ttgtttcgcc gttatctttg acgaaactag gaataaagat atttgcgcca
    20041 gctttggcca acgccatggc aaatgcctgg cctaaaccgc tattcccacc ggtaacaatt
    20101 gcggttttac ctttcagtga gaaaaaatcc attgagaacg cattgagaga ttcgattgac
    20161 atagttagct ccaatttccg taaggcaaaa aaaaaagaaa ggtaacttcc cccgcgagag
    20221 ggaagttacc tttctcatta tctctggtaa ctggagataa tttttagcat aacggcataa
    20281 ctgctaatgt gatcgtaatc acagtgtgat attcgtattg tcaaatagtt ttaaaaagca
    20341 gaagcgtgat tactctcaca caacacctct gctttccatg ttatttctta ttgcaattac
    20401 tagagacctg agaaaagtaa tccacctgaa agggcggctt acttttctct tttttttgtc
    20461 tattacaaat gggaatacga tatgcaacac aactcatatc gccgttggat aaccctcgcg
    20521 ataattagtt ttagcggcgg cgttagtttt gacctggctt atttacgtta tatttatcaa
    20581 attcccatgg cgaaatttat gggattcagc aataccgaga taggtttaat aatgagtacc
    20641 tttggtattg cggctattat tctttatgcc cccagcggcg ttattgccga taaattttca
    20701 caccgcaaaa tgattacttc cgcgatgatc attaccggat tactgggtct gttaatggca
    20761 acgtatccac cgctgtgggt aatgctctgt attcaggtcg cctttgcgat aacgacgatt
    20821 ttaatgctgt ggtcggtgtc gattaaagcc gcctcgttgc tgggcgatca tagtgagcaa
    20881 gggaaaatta tgggctggat ggaagggctg cgcggcgtcg gtgtaatgtc gctggcggtg
    20941 tttaccatgt gggtcttttc tcgctttgca ccggatgaca gcgccagcct gaaaacagtc
    21001 attatcatct acagtgtggt ttacatcttg ttggggattc tgtgctggtt ttttgttagc
    21061 gataacaaca acctgcgcag tgccaataac gaagaaaaac agtcattcca gcttagcgac
    21121 atccttgccg ttttgcgtat cagcaccacc tggtattgca gcatggtgat ttttggtgtc
    21181 ttcacaatct acgccattct gagttactcc accaactatc tgaccgaaat gtacggcatg
    21241 tcgctggtgg cagcgagcta catggggatt gtgatcaaca aaatcttccg cgcgctgtgc
    21301 ggcccacttg gaggcattat caccacctac agtaaagtga aatcccctac ccgcgtgatt
    21361 caaatccttt ccatcatcgg tttgctggcg ttgacagccc tgctcgtcac gaactctaac
    21421 ccccaatcgg tcgcgatggg gattggcctg attttactgc tgggattcac ctgttacgcc
    21481 tcacgcgggc tgtactgggc ctgccccggc gaagcgagaa caccatctta cattatgggc
    21541 accacggtag gtatttgttc ggtgattgga ttcctgccgg atgtcttcgt ttacccgatt
    21601 atcggccact ggcaagacac cctgcccgct gcagaagcct accgcaatat gtggctgatg
    21661 ggcatggcgg cgctcggcat ggtgattgtc tttacctttt tgctgttcca aaaaattcgt
    21721 actgctgata gcgcccccgc aatggctagc agcaagtaag ccaggaaaat tacaggagaa
    21781 aaatcatgtc gaaaaaatac atcataggga ttgatggcgg aagtcagagc acaaaagtgg
    21841 tgatgtacga tctggaaggt aatgtggttt gcgaaggtaa aggcttatta cagccgatgc
    21901 acacgcctga tgccgatact gcagaacatc ctgacgacga tttatgggca tcattatgtt
    21961 ttgcaggtca cgatttgatg agccagtttg ccgggaataa agaagatatt gttggcatcg
    22021 gcctgggctc catccgttgc tgccgtgcgt tattgaaagc cgacggcacg ccagctgcgc
    22081 cgttgattag ctggcaggat gcacgcgtta cacgccctta cgaacatacg aatcctgacg
    22141 tggcgtatgt aacctctttt tcgggttatc tgacgcatcg cttaaccggc gaatttaaag
    22201 ataatatcgc caactatttt ggtcagtggc cggtggatta taagacctgg gcatggagcg
    22261 aagatgccgc agtcatggag aagtttaata tcccccgtca tatgctgttt gatgtgcaaa
    22321 tgccaggcac tgttcttggg catatcacac ctcaagccgc gctggcgacg catttcccgg
    22381 caggactgcc ggttgtttgt accaccagtg ataaaccggt agaagctctg ggagccggat
    22441 tactggatga tgaaactgcg gtaatttctc ttggcactta catcgcattg atgatgaacg
    22501 gcaaagcctt gccgaaagat ccggtggcgt actggccgat tatgtcttct attccgcaaa
    22561 cattgctgta tgaaggttac ggtattcgca aaggcatgtg gacggtgagc tggctgcgcg
    22621 acatgttagg cgagtcgtta attcaggatg ccagggcgca ggatctttca ccggaagatt
    22681 tactcaacaa gaaagcctct agtgtgccac ctggctgtaa tgggctgatg acggtgctgg
    22741 actggctgac caatccgtgg gaaccgtaca aacgcgggat tatgatcggc tttgattcca
    22801 gcatggatta cgcatggata tatcgttcga tattggaaag cgtggcgctg acgctgaaga
    22861 acaattacga caatatgtgt aatgaaatga atcactttgc gaaacatgta atcattactg
    22921 gcggcggttc gaacagcgat ctgtttatgc agatttttgc cgacgtgttc aaccttccgg
    22981 cacgccgtaa cgccattaac ggttgtgcaa gcctgggggc agcgattaat acaggggtag
    23041 gtctggggct atacccggat tacgcaacgg ctgtcgataa aatggttcgc gtgaaagata
    23101 tctttatacc gattgagagc aatgccaaac gctacgacgc gatgaataaa ggcattttca
    23161 aagacctaac caaacatact gatgtgatcc tgaaaaaatc gtatgaagtg atgcatgggg
    23221 aattggggaa ttggggaatg cggattcgat ccagagctgg tcgaatgcgt gaataaggta
    23281 ggccgggcgg aaaactgccc ggcctgaaca tacctgaatg gttatccccg ctggcgcggg
    23341 gaacataagt tcgacaactt aaaaaatgat aacaagtccg gtttatcccc gctgatgcag
    23401 ggaacataat aaaaacttta actagttcgg tttatccctg tttttacaag gaa
//
LOCUS       DAKISA010000068        23377 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.068, whole genome shotgun sequence.
ACCESSION   DAKISA010000068 DAKISA010000000
VERSION     DAKISA010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23377
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.068"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(268..885)
                     /gene="tdk"
                     /locus_tag="OVW45_002694"
     CDS             complement(268..885)
                     /gene="tdk"
                     /locus_tag="OVW45_002694"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCV5527103.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1490..1903
                     /gene="hns"
                     /locus_tag="OVW45_002695"
     CDS             1490..1903
                     /gene="hns"
                     /locus_tag="OVW45_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCV5527104.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2047..2955)
                     /gene="galU"
                     /locus_tag="OVW45_002696"
     CDS             complement(2047..2955)
                     /gene="galU"
                     /locus_tag="OVW45_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCV5527105.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3157..4170)
                     /gene="rssB"
                     /locus_tag="OVW45_002697"
     CDS             complement(3157..4170)
                     /gene="rssB"
                     /locus_tag="OVW45_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCV5527106.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4262..5167)
                     /gene="rssA"
                     /locus_tag="OVW45_002698"
     CDS             complement(4262..5167)
                     /gene="rssA"
                     /locus_tag="OVW45_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCV5527107.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5280..5738
                     /locus_tag="OVW45_002699"
     CDS             5280..5738
                     /locus_tag="OVW45_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCV5527108.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5788..6630
                     /gene="purU"
                     /locus_tag="OVW45_002700"
     CDS             5788..6630
                     /gene="purU"
                     /locus_tag="OVW45_002700"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCV5527109.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMAEAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6790..6874
                     /locus_tag="OVW45_002701"
     tRNA            6790..6874
                     /locus_tag="OVW45_002701"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6824..6826,aa:Tyr,seq:gta)
     gene            6922..7053
                     /locus_tag="OVW45_002702"
     ncRNA           6922..7053
                     /ncRNA_class="other"
                     /locus_tag="OVW45_002702"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7237..7914)
                     /gene="narI"
                     /locus_tag="OVW45_002703"
     CDS             complement(7237..7914)
                     /gene="narI"
                     /locus_tag="OVW45_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCV5527110.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="OVW45_002704"
     CDS             complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="OVW45_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCV5527111.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8621..10159)
                     /gene="narH"
                     /locus_tag="OVW45_002705"
     CDS             complement(8621..10159)
                     /gene="narH"
                     /locus_tag="OVW45_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCV5527112.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10156..13899)
                     /locus_tag="OVW45_002706"
     CDS             complement(10156..13899)
                     /locus_tag="OVW45_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCV5527113.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVSTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFLDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14292..15683)
                     /gene="narK"
                     /locus_tag="OVW45_002707"
     CDS             complement(14292..15683)
                     /gene="narK"
                     /locus_tag="OVW45_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCV5527114.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16022..17818
                     /gene="narX"
                     /locus_tag="OVW45_002708"
     CDS             16022..17818
                     /gene="narX"
                     /locus_tag="OVW45_002708"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCV5527115.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDEQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLLDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17811..18461
                     /gene="narL"
                     /locus_tag="OVW45_002709"
     CDS             17811..18461
                     /gene="narL"
                     /locus_tag="OVW45_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCV5527116.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="OVW45_002710"
     CDS             complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="OVW45_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCV5527117.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEVWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVRVEQYFGERVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20041..20394
                     /gene="ychN"
                     /locus_tag="OVW45_002711"
     CDS             20041..20394
                     /gene="ychN"
                     /locus_tag="OVW45_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCV5527118.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20438..21133)
                     /gene="chaC"
                     /locus_tag="OVW45_002712"
     CDS             complement(20438..21133)
                     /gene="chaC"
                     /locus_tag="OVW45_002712"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCV5527119.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPNE
                     GPVWIFGYGSLMWNPALEFTELCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="OVW45_002713"
     CDS             complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="OVW45_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCV5527120.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21791..22891
                     /gene="chaA"
                     /locus_tag="OVW45_002714"
     CDS             21791..22891
                     /gene="chaA"
                     /locus_tag="OVW45_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCV5527121.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23294..>23377
                     /locus_tag="OVW45_002715"
     CDS             23294..>23377
                     /locus_tag="OVW45_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCV5527122.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIV"
BASE COUNT         5857 a         6116 c         5629 g         5775 t
ORIGIN      
        1 tctggggccg tttttttgtt tacccaaagc aacttttcca taaaccgaca gcattagcct
       61 tcatcatatt tgcgacgatg tataacgcct aaacacaggg atattgtact ttacaggtca
      121 caagtcaacg tcggtgctta agagccctgt gaggcgtata gcggcgttaa aaaactgccg
      181 agaagggtat atagcccgga agaagtgcgt aaaacgaact gacaggataa aagtgccctg
      241 ctcaccctgt cagtaaagaa attcttatta atcgtggcga tgcctttcct gaatagccgt
      301 taatgagccg acttgtaacg cctctttata gtgtttacgg catacagaaa cgtatcgttc
      361 attaccacca attaccacct gctcaccttc gttataaggt ctgcctgctt gatcaagacg
      421 cagcaccatg cttgctttac ggccacaaaa acagatggtt tttaattcaa ccagtttgtc
      481 ggaccatgcc agtaagtatt ggctgccaat aaataattca cctcgaaaat cggtacgtaa
      541 accataacaa agtacgggta tatcgagttg atcgacaacc tccgataatt catatacttg
      601 ttgtctggtt aaaaactggc attcatcaac cagtacgcaa tgaattgcct gctgttcatg
      661 ttccgcacga atctcatcaa ataatgatga attttggtta aataattttg caggcgatga
      721 caaacctata cgcgaactga ctttcccggc accaaagcga tcatcaattt ctgccgtata
      781 tacgacagtg cgcatgccgc gttcctggta attgtatgaa gattgcaaca atgctgtaga
      841 cttacccgca ttcattgcgg aatagtagaa atatagctgt gccatcagcc acaggccctc
      901 aatgatatga aataaagttg gctggagttt atcataattc gtcagttttt cagctcaaca
      961 gtatgcacag aagtgggtat atgcgttctc ccttacgaag ccttgcataa tccttctgag
     1021 ctatcattac aactgcctcg cttgttataa gcgggtaaat gactactggt aactattcac
     1081 aatctttaac ctgttgcgca agtaatagcc ctctgttgac ctccaggaga tagtgcaata
     1141 ctaagtccat gctcttattg cgactgttct acttttcatc attcgcttaa tagggaattc
     1201 tcgtaaacac aactaataca gaagactgaa aggtcgtcag cctacgataa tctccccata
     1261 aaatgtgaca tgaatcagga agttttaacc tcacgtgctg cgaaatcatc ggtgtaaata
     1321 gggctatatg ccgcgtcttt tctggctaat tttatgaaaa gatatttatt ggcggcacaa
     1381 aataaagaac aattttgaat tccttacatt cctggctatt gcacaactga atttaaggct
     1441 ctattattac ctcaacaaac caccccaata taagtttgag attactacaa tgagcgaagc
     1501 acttaaaatt ctgaacaaca tccgtactct tcgtgcgcag gcaagagaat gtacacttga
     1561 aacgctggaa gaaatgctgg aaaaattaga agttgttgtt aacgaacgtc gcgaagaaga
     1621 aagcgcggct gctgctgaag ttgaagagcg cactcgtaag ctgcagcaat atcgcgaaat
     1681 gctgatcgct gacggtattg acccgaacga actgctgaat agccttgctg ccgttaaatc
     1741 tggcaccaaa gctaaacgtg ctcagcgtcc ggcaaaatat agctacgttg acgaaaacgg
     1801 cgaaactaaa acctggactg gccaaggccg tactccagct gtaatcaaaa aagcaatgga
     1861 tgagcaaggt aaatccctcg acgatttcct gatcaagcaa taatcttttg tagattgcac
     1921 ttgcttaaaa tcccgccagc ggcgggattt tttattgtcc ggtttaagac aatttaataa
     1981 gaataagcta taaaaaaacg gcgtcgattg ctcaacgccg tttcgtggat aacaccgata
     2041 cggatgttac ttcttaatgc ccatctcttc ttcaagccag gctttaaatt ccgtgccaag
     2101 ggtgttatga cgaataccgt attcaacgaa ggcctgcatg taacctaatt tattaccgca
     2161 gtcatggctc ttccctttca tatgataggc ttccaccgtt tctttttcga tcagcatatc
     2221 aattgcgtcg gtgagctgaa tttcatcacc agctcccgga ggggtttttg ccagcaacgg
     2281 ccaaatatcc gcgctaagta cgtaacgacc cacaatagcg agattagacg gcgcaacatc
     2341 cgctttcggt ttttctacca caccaaccat cggtacgctt tcacccggcg ctaattcaac
     2401 gcctttgcaa tccacaacgc catatgcggt cacatcagca accggttcaa ccatgatctg
     2461 gctatgaccc gtttcatcaa agcggcggat catctctgcc aggttatcct gtgacaaatc
     2521 ggattcatat tcatccagaa taacatcagg caaaataaca gctaccggtt catcacccac
     2581 taccgggtga gcacacaata ccgcgtgtcc caggcctttc gccagaccct gacgaacttg
     2641 cataatagtc acgtgcggtg gacaaataga ctgcacttca tcaagcagtt gacgttttac
     2701 acgtttttcc agcattgctt ccagttcaaa actggtatca aagtggtttt caatagagtt
     2761 tttagatgag tgtgtaacca gcacaatttc agtaatgcca gccgcaatac attcattcac
     2821 gacgtattga attaatggct tatcgacaag tggcagcatc tctttcggga tggctttcgt
     2881 cgccggcaac atcctggttc ctaatcccgc aacggggata acggcttttt tgactttcgt
     2941 attaatggca gccatttaaa ttctcctgga ctgttcgtgt tttgaacgtg ttcatatttc
     3001 tgtatcgcat cccagtatat cagcaccccg aaggggctaa gttctgaaaa ggaggcgagt
     3061 tagcataatt aagacaatta cttatacgaa tggcgacgat agtaccacca gccgacatta
     3121 gcaggtaatg caaatttagc ccgcgttatc gtttgctcat tctgcagaca acatcaagcg
     3181 cagtcgacca ccggttcccc atatttggca ttgccaggca tcgcatcgct ggctcaattg
     3241 attcaaataa gcgttaccta aagtgcctaa cggaacacca ttactgattt gcacctggtg
     3301 ttcgccggta tttaacgtcg cattcagacc cgcagaaacc agaatgagat ttttcagttc
     3361 gcgatgataa tagccaacta atagcggaaa ctgccccggc agattggcct gacggagtaa
     3421 atggtttacc tgcttcaata acgcgcccaa ctctggcaac cgttgatttt ggtgtgcaag
     3481 ctgttcctgt aataatccgt taaacaatgc gcgtaataac aaggcagcaa gtacgccatt
     3541 atgtccagct cgggtgacat caaggcaata aaatgccaga tcgttttccg aaagtgcggc
     3601 aatatcaagc accaggccgg gtttgtccgc ggcaaccaat tgacgataat taacccggca
     3661 atgggaaatc acctgctgaa ccggcggttg tagttcctgt aataatttcg ccgctgcggc
     3721 agggttatca accattgcat cccagtcgcg aaaaagcctt tcctcttcct caacgcgcga
     3781 attaaacatg ctgggataga gacaggcaaa aaccatctcg cgcaagcgat tcagatcttt
     3841 aactggtttc agcaaaacat cttcaacgcc cagacgtaac gctttggcaa tatctgccat
     3901 attttcagtg gcagatatca ccagaactgg ggtctggtcg cctctgttac gtatatgctc
     3961 cagcagttta agcccgttca ttcgtggcat cgcgatatca catatcatca ggtctggagt
     4021 gaaacctccc agcaactcaa gggcatccac cccatcagcc gccagtaccg ttgtcgctcc
     4081 caatgaggaa aaccatgaat ccagaagcga gcgaaatacc tgctcatctt caacaatgag
     4141 aatctgtttt ccgaccaatg gctgcgtcat gttctctccc ctgactggct ttactcaata
     4201 gtggcatgct attgccacct gcgcctgtca gaattaactt aagtgtagat aaaaattctg
     4261 gtcaaatgtt ggtgcgtacc aacggcaaaa gttcgtccat tttcctttcc actgccagct
     4321 gtccggccgc aatggcagcg tgcgcgcgat ggaaatcaag cgtagatatt tgcgggcaaa
     4381 caggttgaat cagaatatcg ggcggatcac ctgccatgcg gttcctttta aggcggttct
     4441 ccaacacctg gatagacgtg gtcataatct ctgttgccgt tggcgctgtc acagcccgac
     4501 gtgtcgttat gctgcctaac ctttctttca gacgcgcatg ccacggtaga gaatcaccat
     4561 tctcgctatt ttcttcactg acattaaagg agagcagatc ttgttgcatc aaatgagcat
     4621 cgtgctgcag gtcgaccgct atcacaatat cagcccccaa tgcacgcgta agagaaatag
     4681 gaattgggtt aactactgcc ccatcaacca gccagtagcc gttatgtgca acgggtgcca
     4741 tgagtcctgg aatactgcat gatgcgcgaa tagcaagatg gagatcgcct tcagtaaacc
     4801 ataattcacg ccccgtactt aaattggtgg caacagccgc aaagcgacgg gaacaatttt
     4861 cgatctctgt ttccggcatt atttcgcgat attgattgaa gacacgctcg ccgcgcagta
     4921 acccaccgcg ctgccaggag aggtccatca ggcgtaaaac gtcccaataa ctgaaagagg
     4981 ttacccaatc ttccagcgca gataatcgat cgcatgcata ggccgcgccc accagcgaac
     5041 caattgaaca tcctgcaacg atatcaattt caatacccac tttttttagc gcattaataa
     5101 cgccaatatg cgaccaacct ctcgccgcgc cagatcccag cgccagccct atttttatct
     5161 ttctcattat tcccgcaaga ttcccctgga atgcaatcgt agccacatcg ctactgctcg
     5221 gttaacatag tgctactcag gcggttaata cgccgttatt gtttccaggg agattatttg
     5281 tgtctcagct ttgtccctgt ggtagtgctg tcgagtatag cctatgttgc cacccttatg
     5341 tgtctggtga aaaggttgca cctgatccag aacatctcat gcgttcgcgt tactgcgctt
     5401 ttgtgatgca agacgcagat tatttaataa agacctggca tccctcttgt ggagcagcgg
     5461 cgttacgtgc cgaattgatg gccggatttg cgcatactga atggttggga ttgaccgtat
     5521 ttgaacattg ttggcaggac gccgacaata tcggatttgt cagttttgtt gctcgattta
     5581 ccgaaggtgg caaaacaggt gcaataattg aacgttcgcg atttttgaaa gaaaacggtc
     5641 aatggtacta tattgacggt acacgtccgc agtttggtcg caacgatccc tgcccttgtg
     5701 gttcaggtaa aaaatttaaa aagtgctgcg gccaataatg gttgacggta cggtttagca
     5761 aacactctca acaaggtttt tccagcaatg cattcactcc aacgtaaagt tctgcgtact
     5821 atttgtccgg accaaaaagg tctgatcgca cgtattacca atatttgcta caagcacgag
     5881 ttaaatatcg tacagaacaa tgaatttgtt gatcaccgta ccgggcgctt ttttatgcgc
     5941 acggaactgg aagggatttt taatgattcc accctgctgg cggatctcga tagcgcattg
     6001 ccagaaggct ccgtgcgtga gctgaatcct gccggtcgtc gccggatagt gattctggtc
     6061 actaaagaag cgcattgcct tggcgatttg ttgatgaaag ccaattacgg cggcctggat
     6121 gtcgaaatcg cggcagttat tggtaaccac gatactttac gttctctggt tgagcgtttt
     6181 gatattccgt ttgagctggt aagccatgaa gggttaagcc gcaacgagca cgatcaaaag
     6241 atggcggaag ccattgatgc ttatcaacct gactatgtgg tgctggcgaa gtatatgcgg
     6301 gtattaacgc cggaatttgt ggcacgcttc ccgaataaaa tcatcaatat tcaccattca
     6361 ttcctgcctg cgtttatcgg cgcacgtcct tatcaccagg cctatgaacg tggtgtgaag
     6421 attattggcg caaccgctca ctatgtgaat gacaatctgg acgaaggccc aatcatcatg
     6481 caggacgtta ttcatgtcga tcatacctac acagctgaag atatgatgcg cgcaggtcgt
     6541 gacgtcgaga aaaacgtctt aagtcgtgcg ctatacaaag tactggcaca gcgcgtcttt
     6601 gtttacggta atcggacgat tattctttaa tcgccagcaa aaataactgg ttacctttaa
     6661 tccgttacgg atgaaaatta cgcaaccagt tcatttttct caacgtaaca ctttacagcg
     6721 gcgcgtcatt tgatatgatg cgccccgctt cccgataagg gagcaggcca gtaaaaagca
     6781 ttaccccgtg gtggggttcc cgagcggcca aagggagcag actgtaaatc tgccgtcatc
     6841 gacttcgaag gttcgaatcc ttcccccacc accatcactt ttcaaaagtc cgtaaactct
     6901 caaacgaatc cgcaattaaa tattctgccc aagcggggaa ggatgagaag cttcgaccaa
     6961 ggttcgactt gagcgccagc gagagagcgt tgccgcaggc aacgacccga agggcgaagc
     7021 gcgcagcgct gagtaatcct tcccccacca ccatcgcttc cttaaataat caaaacctgt
     7081 tactccgagc taattttgcc cgatggcacc acgtttaccg ggcctacaat atcctcccct
     7141 gtaggtcgga taagcgaagc gcatccgaca actcaccccg caccgtgccc ataaaaaaac
     7201 cccgccgaag cagggtctat gtgaactaaa attcgcttag tgacgagcgc gcaccagctg
     7261 gtacttacgt gtcagatact ccaccggtac gctccagatg tgtaccagac gcgagaacgg
     7321 gaacagcagg aataacgtca tccccagcac caggtgcaga cggaagataa acgccacacc
     7381 atcgaggtgt tgagaagcgc caccgtggaa ggtcaccacc gactgcgccc agccaaccag
     7441 tttcatcatc tcgctaccgt ccatatgctg agcggagaac ggaatggtca acaggcccag
     7501 cgcgcactgg ataacgagca gcgacaggat caggatatcc gctccggtag tggttgcacg
     7561 cacgcgtggg ctgaacagac gacgcttcag caacagcacg ccgccaatca gacacagcac
     7621 gccgctggca ccaccagcaa acatcgccat tttctgtttc acttcaatcg gcagccaggc
     7681 ttcatacatc cagtgcggcg tcagcatacc gaagaagtga ccaacaaaaa tccccagaat
     7741 accgatatgg aacaggttcg acgccaggtt catccctttg cgatccagca tctggctgga
     7801 cgccgcgcgc caggtgtact gcccgtagtc ataacgcagc cagctaccaa tcaggaagac
     7861 cgccccggct atgtacgggt agatatcaaa gaagaacata ttcaggaatt gcattagtgc
     7921 tgtcctccgg tggtgatatt cagatattgc ggcgcgacgg caccggcaaa gcgacgctga
     7981 tgagcagtga ttgctgaatc accgcagcct ttgtcagcaa agaatttaac ctgctcttct
     8041 tcccagacag catccagcgc ctgcggcgta tcatcgcgcg cttcgtcggc aattttttcc
     8101 gccactttgt cgctgtcgat agcggtattg gccaatttca gcagcagatc aaacatcacg
     8161 gcataacggc tttcacgctg ttgcagacgc gcgctcagca atgccagaat cggcgcgata
     8221 tctttcaaac cttccacggc ttcgctttgc ggcagctgcg acaggtactc cagatacagc
     8281 ggcagatggt ccggtaattc gcggctgttt aactgcaagc cgtgctgctc gtactgcgcc
     8341 agcaggtcca ccatcgcctg gccgcggtca cgggattcac cgtgaacatg ttcaaatagc
     8401 aacagtgacg tggcgcggcc acggtcgaac agttcgctgt actgggcctg ggcatcgagc
     8461 ggatccatcg ccgttaaatc gcgcaggaaa atgcccagcg catgggcatc ttctttcgac
     8521 agatttttcg acgcagcaat cgcctcaaac atctcttgtt gatgctgcca taaggcagca
     8581 tccggatatt caaggagacg cgatacaatc acgagttcga tcatggatgc ggctccgttt
     8641 tgctggtcac atcgatggca tcgatacgac ggctgttgaa cagattgaat ttggtatctg
     8701 aaccgtggca gccatcacca aaggtaaagc cgcagccatt tttctccggg aaggcttccc
     8761 gtgccagttc acgatgacta ctcggcacca caaagcgatc ttcgtagtta gcaatcgcca
     8821 gataacggta catctcctgt gcctgggctt cggtcagacc gacctcttcc agcgcacggg
     8881 tatcaacttt accgtcaacg gtttcagcac gtttgtaatg acgcatcgcc agcatacgtt
     8941 tcagtgcgcg cagtaccggt ttggtatcac cggcggtcag cagattcgcc agatactgta
     9001 ccggaatacg caagctttcg acgtctggca gaatgccgtt gctacccagc tcacccgcgt
     9061 ctgctgcaga ctgaatcgga gacagaggcg gcacgtacca gaccatcggc agtgtgcgat
     9121 attctggatg cagcggcagc gccagtttcc attccattgc cattttataa accggcgact
     9181 gctgtgcggc ttcaataacg ctcagcggaa taccgtcttt aatcgcctgc tcgatgactt
     9241 tcggatcgtt cggatcgagg aacacctcca gctgacgctg gtaaagatct ttctcgttct
     9301 cggtgctggc tgcacgttca atcgcgtcgg catcgtacag cagcacgcca agataacgga
     9361 tacgaccgac acaggtttct gagcacacgg tcggctgacc cgcttcaata cgcggatagc
     9421 agaagatgca cttctcagac ttaccgctct tccagttgaa gtagattttt ttgtacgggc
     9481 atccggtgat gcacatacgc cagccacggc atttatcctg gtcgatcagg acgatgccat
     9541 cttcttcacg cttgtaaatc gcaccgctcg ggcaggtcgc cacacatgcc gggttcaggc
     9601 agtgttcgca caggcgcggc aaatacatca taaaggtgtt ttcgaactgg ctatacatcg
     9661 ccttctggat gttgtcgaag ttcttgtctt tcgccagttt gtcaaactca ccacccaggt
     9721 catcttccca gttcggccct ttttcgattt tcgccatccg ttccccggta atcagcgaac
     9781 gcggacgggc aatcggctgc gatttgctgc cttccggcgc ggtatgcagg ttctgatagt
     9841 caaaatcgaa cggctcgtaa taatcgtcga tccccggcag atgcgggtta gcgaagattt
     9901 tacccagcaa catggcacgg ttacccatgc gcggctgcag tttgccgttg attttacgga
     9961 tccagccgcc tttgtatttt tcctggtttt cccagtcagt cgggaagccc tgacccggct
    10021 tggtttccac gttgttgaac cacgcgtatt ccacgccttc acggctggtc cagacgtttt
    10081 tacaggtaac tgaacaggtg tggcagccga tgcacttatc gagattcagc accatgccga
    10141 cttgtgaacg aattttcatt ttacgctctc ctgtacctgg tcattgcctt cgccatctaa
    10201 ccagtcaatg ttcttcatct tacgcactac aacaaactca tcgcggttag aacctacggt
    10261 gccgtagtag ttaaagccgt atgccagatg ggcatagccg ccgatcatat gcgtcggttt
    10321 cggcgtgata cgggtgaccg agttatggat accaccacgc tgttgggtaa tttccgaacc
    10381 aggcaggtta acgatacgtt cctgcgcgtg gtacatcatg gtcatccctg ccggaacacg
    10441 ctggctgaca accgcacggg cagtcagagc accgttgctg ttgaatactt caatccagtc
    10501 gttatcggcg atacccagat ctttggcatc ggcttcactc aaccagacca ccggaccacc
    10561 gcgacctaaa gtcagcatca gcaggttgtc gctgtaggtg gagtggatac cccacttctg
    10621 gtgcggcgtc aggaagttga gcgctttttc cgggttgccg ttggatttct ggcctatcac
    10681 ttctttcacc gaacgggtgt cgatcggcgg acgataaacc agcaggcttt caccgaaatc
    10741 acgcatccac tggtgatcct gatacagttg ctgacgacca gagagcgtac gccatgggat
    10801 cagctcgtga acgttggtgt aaccggcgtt gtaagaaacg tgttcatctt ccagaccaga
    10861 ccaggtcggg ctggagataa ttttgcgcgg ctgtgcctga atatcgagga agcggatctt
    10921 ctcgtcttct ttattcagcg ccagatgcgt atggtcacga ccggtaaatt cgctcagggc
    10981 agcccaggct ttcacggcta cctgaccgtt ggtttccggt gccagtgtca ggatcatctc
    11041 tgccgcatca attgcggtgt tcagcatcgg ctggcctttc gccggacctt ccgctttggt
    11101 gtagttgagc ttacgcagca gatccatctc gctctgggtg ttccaggcaa tccctttacc
    11161 gccattaccg attttctcca tcagcgggcc aatagaggta aagcgttcgt aagtcgccgg
    11221 ataatcgcgc tctacgacca taatgtgtgg cgcggtttta cctgggatca ggtcgcactc
    11281 gccttttttc cagtctttca catccagcgg ctgcgccagt tcagcggcag agtcatgctg
    11341 gataggcagc gtgacgatgt cggtttcttt acccagatgg ccgacgcaca cttcggagaa
    11401 tttcttcgcg atggctttgt agatttccca gtcgcttttc gcttcccagg ccggatcgac
    11461 cgccgcagac agcgggtgaa taaacggatg catatccgaa gtattcatgt cgtctttttc
    11521 gtaccaagtc gccgtcggca aaatgatgtc ggaatagaga caggtgctcg acagacggaa
    11581 gtccagcgta accaccagat ccagcttgcc ttccagacca ttgtcctgcc agtccacttc
    11641 ttccggcttc acgccgccct gttgccccag atctttaccc tggataccgt gctccgtccc
    11701 cagcaggtac ttgagcataa actcatgacc tttaccggaa gaaccgagca ggttagaacg
    11761 ccagatgaac aggttgcgcg ggtggttttt accgttttct ggttgttctg ccgcaaaacg
    11821 gatggaaccc tctttcaggg atttcaccgt atagtccacc ggattcatcc cggctttttc
    11881 cgcttcgcca gcgatagtca gcgggttagt gcctaactgc ggtgcagacg gcagccagcc
    11941 catgcgttcc gcacggacgt taaagtcgat caagtgtccg gtatagcggg atttgtccgc
    12001 catcggtgac agcaactctt ccgccgtgac ggtttcataa cgccactggc tggagtggtt
    12061 atagaaataa gaagtgctgt tcatgtgacg cgccggacgc tgccagtcaa gggcaaacgc
    12121 cagcggctgc cagccggttt gcggacgcag tttttcctga cctacatagt gcgcccagcc
    12181 gcccccgctc tgaccgacac agccgcagaa aatcagcatg ttgatcagac cacgatagtt
    12241 catatcgagg tgataccagt ggttcagccc cgcaccgacg ataatcatcg aacgaccgtg
    12301 cgttttatca gcgttatcgg caaattcacg ggcgatgcga ataatctggc tgcgagaaac
    12361 gccggtaatc tgctcggccc aggccggggt ataagctttc acatcgtcat agctggttgc
    12421 acagttaacg tcgttcaggc cacgttccag accgtagttt gccagcgtca gatcataaac
    12481 ggtgctcacc agggcggtgc tgccatcagc cagttgcagg cgtttcaccg gcagtttgtg
    12541 cagcagcacg ttttccagtt ccactttgtt gaagtgttcc gtgccgtcgc caccaaagta
    12601 cgggaagcct acctcagcga tctcatcctg gctacccagc aggctcagtt gcagctcggt
    12661 ttcttcgcca gttttgccgt cgcgctgctc aagattccat ttgcccttct cgccccagcg
    12721 gaagccaata gaaccgttcg gcgcaaccat ttcgccattg gtattaaagg cgacagtttt
    12781 ccattccgga ttgttttcct ggcccagcgc atcaaccaga tcagcagcgc gcagcatacg
    12841 acctgcagcg tagtaaccgt cgcgttcttc cagcatcacc agcatcggca tgtcggtgta
    12901 gcgacgcaca tagtcggtga aatactggct tgggttgtcg aggtggaatt cacgcagcat
    12961 tacgtggccc atcgccagcg ccattgccgc atcggtgccc tgtttcggtg ccagccacag
    13021 atcgcacagt ttggcgattt cagcgtagtc tggtgtgacg gcaacagttt tggtgccttt
    13081 gtaacgcact tcagtaaaga agtgagcatc cggggtacgc gtctgcggca cgtttgaacc
    13141 ccaggcgatg atgtagctgg agttatacca gtcagcagat tccggtacgt cagtttgctc
    13201 gccccaggtt tgcggagacg caggaggcag gtcgcagtac cagtcgtaga agcttaagca
    13261 agtaccgcca atcagcgaga gatagcgtgc acccgatgcg taagaaacca tcgacattgc
    13321 cggaattggc gagaaaccag caacacggtc cgggccgtag tttttgatgg tgtaaacgtt
    13381 agatgcggcg atcagttcgt tcacctcctg ccaggaagaa cgaacaaatc caccgcgtcc
    13441 acgcgcctgc ttaaagcttt tcgctttatc ggcgtcttca atgatagaag cccatgcctc
    13501 aaccggatcg ctatgcagcg ccttcgcttc acgccacatt ttcatcaggc gtttgcgcat
    13561 catcgggtat ttcaggcggt tggcactgta aagataccag gagtagctgg caccgcgcgg
    13621 gcagccgcga ggttcatggt ttggcagatc cggacgggta cgcggatagt cagtctgctg
    13681 ggtttcccag gtgaccagac cgtttttgac gtagattttc cagctgcagg agccggtgca
    13741 gtttaccccg tgggtagagc ggacgatttt gtcatgctgc caacgctggc gatatccatc
    13801 ctcccagtca cggttggtat tgagaagctg gccatgccca tcggcaaagg tttcaccctt
    13861 ctgtttgaag tagcgaaacc ggtccaggaa tttactcatc ggttttctcc tgtgggagcc
    13921 tgtcggccct ctgataaatc gacattgcta atgtttaagg gcaaggtaac gctctgaaac
    13981 agcgtgggaa ttgataacga tcaagagtga tggggatgaa aaataaagta aattcctaag
    14041 ataccccttt aggataatta acagccacca cataacagac tgtaaattaa gacgttttat
    14101 acctgtagtt atttaaacta ttaagatttt tatgcagatg ggtattaagg agtattcccc
    14161 atgggtaaca tattaatggc tcttaatgaa atccggcgaa ataagccata taatatgagt
    14221 atgttgtaac taaatgacgc ataaaaaaag cgcgacatca tgccgcgcaa aggataatca
    14281 aatattaaga tttatttttt agaatgccga ccatataccg cccaggtaat cactacgcag
    14341 gcgatataga aaatcaaaaa taccttcatt gcgccgactg gcgaacccgt taatgccagc
    14401 gagctaccaa acgctttcgg gataaagaag ccaccaatag cgccaatcgc agagatgaaa
    14461 cccagcgccg ctgccgtgtc ggttgccgct tcacgcatcg cacgttcgtc agaaccccct
    14521 tctgctttca cgcgatccat tgtcagttta cggaagatca ctgaaatcat ctggaaagtg
    14581 gaaccactac ccagcccagc tgtcaggaac agcgccagga agaccgcgaa gaacgccatg
    14641 aagcttccgc cctgcccgtc agtcggtaag gtcaggaaca gcaggccgct gaaaatcgcc
    14701 atcagaataa agttcaccag cgtgacacga gttccgccca gacggtcaga taatgcacca
    14761 cctgcagaac gcgccagcgc accaataaac ggcccgaaga aagcgtattg cagaatctga
    14821 acatccggga actgcgtttt tgacagcatc gcaaagcccg cggagaagcc gataaaggag
    14881 ccgaaggttg ccagatacag caggctcata atccacagat gaccccgttt gagtaccggc
    14941 aactgctcct tgatggaggc tttcgaggta gcaagatcgt tcatgccaaa ccacgccgca
    15001 atggtgaaga tggcaaggaa cggcacccat atccaggacg cattcgccag atacagctca
    15061 gtcccatccg gctgtttgac gccctggcta ccaaatactg cgaaaatcga cagtgatacc
    15121 accagcggag caaccaactg catgacgctg acgcccatgt ttcccagacc accattcaga
    15181 cccagcgcgc caccctgctt ctgtttcgga aagaagaagc tgatgtttgc catactggat
    15241 gcgaagttcg cgccagcaaa gccgcatagc agagagatga tgatgaagac gctataaggc
    15301 gtggaggtat cctgcacggc aaaacccagc cagacgcaag gaataatcag aataccggtg
    15361 ctgaacgccg tccagcgacg accaccgaag ataggaacca taaaggagta tggaacacgt
    15421 aataacgcgc cagaaaccga aggcagcgca gtcaacataa atagctgatc ggtcgtaaaa
    15481 ttaaaaccga ctttcggtaa gttcaccgca acagcgctga acaacatcca tacgcaaaac
    15541 gccagcagca gacagggaac ggaaatccac aggttacggc tggcaatacg ttgaccgcgt
    15601 tgttgccaga acgcaggatc ttccggtcgc caatctgtaa tgacagctcc agtagccctt
    15661 tcgggggcgg atgagtgact catagacacc tctgatactc gtttcgatgt ctgccacctt
    15721 agtgtctgta gctaaaggca atttgatgta aatcaaacga taagataact ttatctatca
    15781 ttgatattta tcattaccca tagtgagtac agtgatttca taaaaattat gagatttttc
    15841 acggtgctgt aaaatcccta cccttaccga tgtaaagcga ctaaccacac ggcaaataag
    15901 gagtaactct ttccgggtat gggtatactt cagccaatag ccgagaatac tgccattcca
    15961 gaatgtatcg tcacattcat taaggttatt gctcatttaa agcctgaagg aagaggtttg
    16021 catgcttaaa cgttgtctct ctccgctcac cctggttaat caggttgcgc ttattgtgtt
    16081 gctttctact gctattggac tggcagggat ggcggtttct ggctggctgg tgcaaggcgt
    16141 tcagggcagc gcccatgcga tcaacaaagc gggatcgctg cgcatgcaaa gttaccgtct
    16201 gttggcggca gtgccattaa gtgagaaaga caagccctta attaaagaga tggaacaaac
    16261 ggcatttagc gccgagttaa ctcgagcagc agaacgagac gaacaactgg cgcaattaca
    16321 gggtttacaa gattactggc gtaatgaact gatccctgca ctgatgcgtg cacaaaaccg
    16381 cgaaacggtg tcagcggatg tcagccagtt tgttgccggg cttgatcagc tggtatctgg
    16441 ttttgaccgc accacggaaa tgcgcattga gacagtggta ctggtccatc gggtaatggc
    16501 ggtatttatg gcacttttac tggtgttcac tattatctgg ttgcgggcgc gactgctaca
    16561 accgtggcgg caactgctgg caatggcgag tgccgtcagt catcgcgatt ttacccaacg
    16621 cgcgaacatc agcgggcgca acgaaatggc gatgcttgga actgcattga acaatatgtc
    16681 tgcagaactg gccgaaagtt atgccgtact tgagcagcgg gttcaggaga aaaccgccgg
    16741 gctggagcat aaaaatcaga tcctctcttt tttatggcag gctaaccgcc gtttgcattc
    16801 ccgcgccccg ctgtgtgaac gcctgtcacc tgtactcaac ggcttacaga atttaaccct
    16861 gctacgtgat atcgaattgc gggtgtatga cactgatgat gaagagaatc atcaggagtt
    16921 tacctgccag ccagatatga cttgtgatga taaaggctgc cagctctgcc cgcgcggcgt
    16981 attacccgtt ggtgatcgcg gcacgaccct gaagtggcgg ctggctgact ctcatacgca
    17041 gtacggtatt ttgctggcga ccctgccgca ggggcgtcat cttagccatg atcaacaaca
    17101 actgctggat accctggttg aacaactcac cgccacgctg gcgctggatc gccatcagga
    17161 acgtcagcaa cagttgatcg tgatggaaga gcgtgccacc attgcgcgcg aactgcatga
    17221 ttctattgcc caatctctct cttgcatgaa gatgcaggtg agttgtttac agatgcaggg
    17281 cgatgcgctg ccagaaagca gccgcgaact gttaagtcag atccgtaacg aactgaatgc
    17341 atcctgggcg cagttgcgtg aattgctcac cacattccgc ttgcagctca ccgagcctgg
    17401 attacgtccg gcgctggagg cgagttgcga agagtacagc gccaaatttg gcttcccggt
    17461 gaagctggat tatcaattgc cgcctcgcct ggtgccttcg catcaggcaa tccacttgtt
    17521 gcaaattgcc cgtgaggcat taagtaacgc cctcaaacat tcgcaagcga gtgaagtcgt
    17581 ggtgacggtg gcgcaaaacg ataatcaggt caaactgacc gtccaggata acggctgcgg
    17641 cgtgcctgaa aatgccatcc gcagcaatca ctacggcatg ataataatgc gcgaccgtgc
    17701 gcaaagttta cgaggcgatt gccgcgtccg ccgtcgtgaa tcaggtggca ccgaagtggt
    17761 ggtcaccttt attcccgaaa aaactttcac agacgtccaa ggagataccc atgagtaatc
    17821 aggaaccggc tactatcctg ctgattgacg atcacccgat gctgcgaact ggcgtaaaac
    17881 agcttatcag tatggcacca gatatcaccg tggttggcga agcgagtaat ggcgaacagg
    17941 gtattgaact ggcggaatct cttgatcccg atctgatcct gttagatctc aatatgcccg
    18001 gcatgaacgg tctggaaacg ctggataaac tgcgcgaaaa gtccctctca gggcgcattg
    18061 tggtattcag cgtctctaac catgaagaag atgtggtcac cgcactgaaa cgcggcgcgg
    18121 atggttatct gttaaaagat atggaaccgg aagatctgct gaaagcattg catcaggcag
    18181 ctgctggcga aatggtatta agcgaagcat taacgcctgt tctggccgcc agcttgcgcg
    18241 ctaaccgtgc cactactgag cgcgatgtta accagttaac cccacgcgag cgcgatattc
    18301 tcaagctgat tgcccagggt ttgccgaaca agatgattgc ccgccgcctg gatatcaccg
    18361 aaagcacagt aaaagtgcac gtcaagcaca tgctgaagaa aatgaagctc aagtctcgcg
    18421 tggaagcagc ggtatgggtg catcaggagc gcattttctg attacggttc ccagcgcagt
    18481 tcgtcgtttg acaatgcatc gaacggttcg acaagcgtta gcgtgatctc attggaggag
    18541 acacgctgcc cctggttatc ttccaccacc accgacaatc gccagtgatt gctcgcccct
    18601 tccccgttct gccagtcagg catgatcagc gtccagccct ccgcgctgtt ggcttgtgcg
    18661 cccggcgtca aacttaatat ctgcgtatcg ccctgccaaa tcagttgccg aataccgtaa
    18721 cgactgcgga tttgtaattt cagcggcact gtttcgccag gttttagatc ccacggcggt
    18781 gtcgccagaa acaccgttaa cgtttttcgc tgtcggtact caagagtcgg tagattattt
    18841 cgctgcggat tatcatagcg actaccacgt aacgactgac tttcggcaac ctcgcccgca
    18901 gaaagttgtt ttttgagcgg cacaccaaag cggtaattaa gattcagtcc gaggttattt
    18961 tgattttcgc cactttcacc ctgtttatgc tgggccgtca cagtgactaa tggcacaggg
    19021 gtgtaattta atcccagact caacgcgacg ggattgtgat aacccgtacc agagttaaac
    19081 aaatcaacac gctcaccaaa atactgttct accctgacac tggtattgag gtgttgatag
    19141 aacggcatgc gcatccgggc tgtcaggtca tacccgcgcg ccatccgttg ttcctgcgtg
    19201 gctgtctgtt catgccatgc agcaaacggc tgataaaagt ttgccgatag tcgcaaatat
    19261 tcgccccaca cttcggcacc aaagcccgct cgctgaagat tttcgtccag caagttgtca
    19321 taaaaagtgt tataacccac cagccagttg ccgcgcgccc agcgttgccc aacgcccaca
    19381 ttgctcacca acccatcatc ctgctgagta agaccaagct ggctccaggt gagataacga
    19441 tcattatctt gtaacggcac aaaccagctt ccacgactgc cggtgaaatg tccttcgtta
    19501 tccactttga cgtcaacact ggcatttccc cacggtgata gccaggactc tacgtgctga
    19561 ttaacctgtt gactaagcgc gtcgcggact tttcccaatg cgaaagcttt tgcctgctcg
    19621 ccagtatcca gcccgttatc attcatactg gtttcgccga aatctttcac aatttcagca
    19681 aagtgttttt ccccatcatg gttttcggga gccatgccta aatccggcag accatcattg
    19741 ttattatcaa agggatttgc cgctttttgc tcgaaggtag attgtgcgtt agctgtaccg
    19801 cctgccgcca gcaagagtaa ataaaacggg ataatgcgag gaacgaaacg gctcaaaacg
    19861 tcggtaactt ccggtaataa tgaaaaacca tagccttaaa gaataacgat tactggcaac
    19921 ttttgtcgta ttttctcgcg ctttcaggaa aatccttaaa aaggtgaccg attggtcaga
    19981 cgctgcagaa gcagtaagat agaagatgag tgtctaattc cctgaataca ggagtaaata
    20041 atgcaaaaaa tcgtgatcgt tgccaatggc gcaccttacg ggagcgaatc cttgtttaac
    20101 agcttgcggc tggccattgc gttacgagag caggagagca atctggatct gcgtctgttc
    20161 ctgatgtctg atgcggtcac agccgggttg cgcgggcaaa aaccagggga aggctacaac
    20221 attcagcaaa tgctggagat ccttaccgct cagaatgtac cggtgaaatt gtgcaaaacc
    20281 tgtaccgacg ggcgcgggat tagcacactt cctcttattg atggggtgga aatcggtact
    20341 cttgtggaac tggcgcaatg gacgctgtca gccgataaag tgctcacatt ttaatgtttg
    20401 agggggcgtc tgacgcccca ctatgaggga agtttactca ggcgaatccc ggccgtaaca
    20461 caccatccgg ataattctcc gccagcagtt tttttaccga taccagcaaa tcattcagcc
    20521 catcgtcctg cattcccagt ttgataagct cctgttccag tgaaaacagg tattgtgcgt
    20581 tggttcccag cggaccgctc gccgccgcaa tcaacggcgc gatgacctga gcgcgagtgt
    20641 cagattcata ttctggatgt cgcgggtcca taataaacac aatggcgttt actgtgcgtc
    20701 catcatcaag atcaagctga caccaggttg gcagataaca gccggtaatc atctcgcgct
    20761 tccacaacag ggttagttcc tgctccagcg tctcttctgg tagtcgatag gcgacgcctg
    20821 tggtgcgtcc gccctctttc agtgcaagca tccgtcccgg ctggtgcgca gtcccgcgcc
    20881 cggcggtcag gcgcaggcaa aacgcgcgat gccatccaac cagtgtaccg gtgcataatt
    20941 cggtaaactc cagtgccgga ttccacatca acgatccata accgaagatc cacaccggtc
    21001 cctcattagg tcgacaagcc agcgtcgccg ccagcgaagc cgcccgttgt tctgctgacc
    21061 ataagagtga ttcttcaatg gcaccaaatg ccgttttaca atcggcattc atcaagaaat
    21121 cacgcgttat caccttacac ctccgccact gcatacttcc ctgcgacaac tttttggtgt
    21181 cttccggaca tttttaccgc tcattttgaa accactaacg ggacaatatg caatccacgg
    21241 accacttgca aggcaacacc gcacgaatag ctttaactaa gctaccggtt ttacgatttt
    21301 ttatgccatt tatcatcatc ccctttggca tattcatgct tcacagcagc ccaggccact
    21361 ttatgcgcgg tttcttcgcg actggcgtca tcacgccgat cttctttatc tttatattga
    21421 tcccatgcgc tgttgaacgc ttctttatag atatcctggg catgagacgg tagaacgtgc
    21481 tttacgcttt ccggcagatc gcttttcgtt ttatacggca tcgttgactc cttttacaaa
    21541 gttgaacatt aagtgtggtc aacaatatct actcttgcca aaacataaga attttcatgt
    21601 cagactgttc ttaagaaacg ctaaaacaaa cattgtctaa acgtaatact tatttttatg
    21661 ccccaatgag taaaaaatcg acagcggggt aaaaattcgt aaaatgttac cgatatccag
    21721 acactttctg ttatttttcc tacctatttt ttacattttc gataaattca tctgcaaagg
    21781 gataaccata atgtcaaatg ctcaagaggc ggtaaaaacc cgccacaagg agacttcgct
    21841 tattttcccg gttctggcgc tggtagtgct gttcctgtgg ggaagcagcc agacactacc
    21901 agtggtcatt gccatcaatc ttcttgcgct tattggtatt ttaagtagcg cctttagtgt
    21961 tgtccgtcat gcggacgtat tagcccatcg cctgggagaa ccttacggtt cgcttattct
    22021 tagcctttca gtggttattc ttgaagtcag tttgatttca gctttaatgg caaccggcga
    22081 cgccgcgcca acgctaatgc gtgatacgct ctattcaatc attatgattg ttaccggtgg
    22141 gctggttggc ttttcattat tgttgggcgg tcgtaagttt gccacccaat atatgaatct
    22201 gtttggtatc aagcagtatt taattgcgct gttccccctg gcgataatcg tactggtatt
    22261 tccaatggct ctgcctgcgg cgaatttttc aaccggtcag gcgttactgg tagcattaat
    22321 ttctgcggca atgtatggcg tatttttgct gatccagacc aaaacgcatc aaagtttgtt
    22381 tgtctacgag cacgaagatg acagtgatga cgacgacccg caccacggta aaccgtctgc
    22441 ccatagcagc gtgtggcatg ctatctggtt gattatccat ctgattgccg ttattgcagt
    22501 caccaaaatg aatgccagcc cgctggagac attgctcgac agcatgaatg cccctgtcgc
    22561 ctttactggc ttcctggtgg ctctgttgat tctgtcgccg gaaggtttag gtgcgttaaa
    22621 agcagtgttg aacaaccagg tccagcgcgc gatgaatctg ttctttggtt cagtgttagc
    22681 aaccatttcg cttaccgtac ctgtcgtcac gctaattgcc tttatgacgg gtaacgaatt
    22741 acagtttgca cttggtgcgc cagaaatggt ggtgatggtg gcctctttag tgctgtgcca
    22801 tatctccttc tccaccggac gtactaacgt gctcaatggc gcagcgcatc tggcactgtt
    22861 tgccgcctat ttgatgacga tatttgcctg acagttatat ctcctccgat gacggaaatc
    22921 gggggagata agataaattc ctgcttattg tatttcttca tactacctgg acagctcgtt
    22981 aatcagaatt gcgagcctgt accacaacct gcacgaaacc actgcaacag ctgttgaaaa
    23041 gccggaatgt actctttatt gcaaacattt cagggatgaa gtgaagatga attgagacgt
    23101 agtatccaca ccaagccgga attatccggt gaggcgcaat gttgcggggg ctttatccct
    23161 ggtggcattg gttgctggaa agagaaaacc cccgcacgtt gagaggtaaa acctgacaac
    23221 aaaacggggg ctaatcttga aaccagacac ttcaagaata gccgccttgt tgtcattaca
    23281 acaaggcggc tatatgacgc tcgcgcagtt tgccatgact ttctggcacg acctggcagc
    23341 accgatcctg gcggggatta ttaccgcagc gattgtc
//
LOCUS       DAKISA010000069        23238 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.069, whole genome shotgun sequence.
ACCESSION   DAKISA010000069 DAKISA010000000
VERSION     DAKISA010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23238
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.069"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(41..241)
                     /gene="glgS"
                     /locus_tag="OVW45_002716"
     CDS             complement(41..241)
                     /gene="glgS"
                     /locus_tag="OVW45_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HCV5527123.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            510..1139
                     /locus_tag="OVW45_002717"
     CDS             510..1139
                     /locus_tag="OVW45_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HCV5527124.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYNSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGVKVLIICRLSATRYLAENNPWPQIL"
     gene            1166..2827
                     /locus_tag="OVW45_002718"
     CDS             1166..2827
                     /locus_tag="OVW45_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCV5527125.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            3066..3125
                     /locus_tag="OVW45_002719"
     CDS             3066..3125
                     /locus_tag="OVW45_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCV5527126.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            3442..3501
                     /gene="ibsE"
                     /locus_tag="OVW45_002720"
     CDS             3442..3501
                     /gene="ibsE"
                     /locus_tag="OVW45_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HCV5527127.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(3621..5054)
                     /gene="hldE"
                     /locus_tag="OVW45_002721"
     CDS             complement(3621..5054)
                     /gene="hldE"
                     /locus_tag="OVW45_002721"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCV5527128.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPSSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(5102..7942)
                     /gene="glnE"
                     /locus_tag="OVW45_002722"
     CDS             complement(5102..7942)
                     /gene="glnE"
                     /locus_tag="OVW45_002722"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCV5527129.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEEPLSAQAKSVLTFSDFVQD
                     SIIAHREWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSCSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMNNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLISDFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALNHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGRTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSGDCFTAERDLVRASWQKWLVEE"
     gene            complement(7965..9266)
                     /gene="ygiF"
                     /locus_tag="OVW45_002723"
     CDS             complement(7965..9266)
                     /gene="ygiF"
                     /locus_tag="OVW45_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCV5527130.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQFPSEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDLYRDQLPRLTRDIDSILLLAGYYDPVVAH
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            9508..10128
                     /locus_tag="OVW45_002724"
     CDS             9508..10128
                     /locus_tag="OVW45_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCV5527131.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            10192..11430
                     /gene="cca"
                     /locus_tag="OVW45_002725"
     CDS             10192..11430
                     /gene="cca"
                     /locus_tag="OVW45_002725"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCV5527132.1"
                     /translation="MKIYLVGGAIRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTDFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(11593..12414)
                     /gene="bacA"
                     /locus_tag="OVW45_002726"
     CDS             complement(11593..12414)
                     /gene="bacA"
                     /locus_tag="OVW45_002726"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCV5527133.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDISMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(12505..12873)
                     /gene="folB"
                     /locus_tag="OVW45_002727"
     CDS             complement(12505..12873)
                     /gene="folB"
                     /locus_tag="OVW45_002727"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCV5527134.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAM
                     ARAANVGVIIERGNNLKENN"
     gene            12978..13595
                     /gene="plsY"
                     /locus_tag="OVW45_002728"
     CDS             12978..13595
                     /gene="plsY"
                     /locus_tag="OVW45_002728"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCV5527135.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(13608..14540)
                     /gene="ttdR"
                     /locus_tag="OVW45_002729"
                     /gene_synonym="dan"
     CDS             complement(13608..14540)
                     /gene="ttdR"
                     /locus_tag="OVW45_002729"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HCV5527136.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQWLGKPDEGYQVM"
     gene            14747..15658
                     /gene="ttdA"
                     /locus_tag="OVW45_002730"
     CDS             14747..15658
                     /gene="ttdA"
                     /locus_tag="OVW45_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HCV5527137.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            15655..16260
                     /gene="ttdB"
                     /locus_tag="OVW45_002731"
     CDS             15655..16260
                     /gene="ttdB"
                     /locus_tag="OVW45_002731"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCV5527138.1"
                     /translation="MKKILTTPIKAEDLQDIRVDDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            16309..17772
                     /gene="ttdT"
                     /locus_tag="OVW45_002732"
     CDS             16309..17772
                     /gene="ttdT"
                     /locus_tag="OVW45_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCV5527139.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLIVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(17815..18828)
                     /gene="tsaD"
                     /locus_tag="OVW45_002733"
     CDS             complement(17815..18828)
                     /gene="tsaD"
                     /locus_tag="OVW45_002733"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCV5527140.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            19065..19280
                     /gene="rpsU"
                     /locus_tag="OVW45_002734"
     CDS             19065..19280
                     /gene="rpsU"
                     /locus_tag="OVW45_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCV5527141.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            19391..21136
                     /gene="dnaG"
                     /locus_tag="OVW45_002735"
     CDS             19391..21136
                     /gene="dnaG"
                     /locus_tag="OVW45_002735"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCV5527142.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            21331..23172
                     /gene="rpoD"
                     /locus_tag="OVW45_002736"
     CDS             21331..23172
                     /gene="rpoD"
                     /locus_tag="OVW45_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCV5527143.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5656 a         6333 c         5906 g         5343 t
ORIGIN      
        1 tctaatacaa acactaaaac gggccatcag gcccgttata tcagtgctct aactccagct
       61 ctcttgcctg catcatctgt tggcaatacc aggcataacg tgcgcaaaac caggttctat
      121 gttcttcatc catgttacca gtaacggcaa gaatatcgac cgggcgacct tctgcgtgca
      181 ttctggcaaa actacgcgcc aggaaatcga aattatttaa agaattaaga ctatgatcca
      241 taagcacatc ctccggttat cgcatacctt ttaaggccat atgctttttc tgatttcagt
      301 attccccaat cagtgtagtg cctttaaaca taacgtgcgt aaatatattg tccccgatca
      361 cactttttag tgaaaaataa aaaactatat taaaaaactt tctcttccag aacttctcta
      421 acatgtgtta ttattaacaa agataaatgt aagcaaggaa actatcccaa agtaaatatt
      481 gcgtcactaa atggacattg gagtgacata tgattttatt cgccgactat aatacccctt
      541 atcttttcgc tatttccttt gtactgttga tcggcctttt agaaatttta gcgcttatct
      601 gcggtcacat gctctccggt gctcttgatg cacatcttga ccattataat tccataacca
      661 caggtcatat tagccaggca cttcattatc tcaatatcgg aagattgcca gctctcgtcg
      721 ttctctgctt gctggctggt ttttttggtc ttattggcat cctgttgcag catgcctgca
      781 tcatggtctg gcaatcacca ctgtcgaacc tgttcgtggt tcctgtcagt ttactgttta
      841 cgattattgc ggtgcattat accggtaaaa tagttgcgcc ctggattcca cgcgaccata
      901 gttcagctat tacagaagaa gaatatattg gtagcatggc attaattacc ggtcatcaag
      961 ccacttcagg taatccctgc gaaggaaaac tcaccgatca atttggccaa atacattatt
     1021 tactactgga gcctgaagaa ggaaaaattt tcactaaagg agtcaaagtg ttaattattt
     1081 gccgactttc tgcaacgcga tatttggcgg aaaacaatcc ctggccacaa atattgtaat
     1141 ttattaatca aaaggaaatt taataatgga tgatattgtt aattctgtgc cctcctggat
     1201 gtttaccgcg attattgccg tatgcattct gtttattatt ggaattattt tcgccaggct
     1261 ctatcgtcgc gcttcggcag agcaagcttt tgttcgtact ggtttaggtg ggcaaaaagt
     1321 ggtgatgagc ggtggcgcaa tcgtgatgcc gatctttcat gaaataatcc ccatcaatat
     1381 gaatactctg aagctggaag tcagccgctc aaccattgat agcctgatta cgaaagatcg
     1441 tatgcgtgtc gatgtagtag tcgctttctt tgtacgggta aaaccgtctg tagaaggcat
     1501 cgccactgcg gcgcagacgt tggggcaacg cacgctatca ccagaagact tacgtatgtt
     1561 ggttgaagat aaatttgtcg atgccctccg tgcaacagct gcacaaatga ccatgcatga
     1621 gttacaggat acccgcgaga acttcgtgca gggagtacaa aatactgtcg cagaagacct
     1681 atcgaaaaac ggtcttgagc tggaaagcgt ttcacttacc aactttaacc agacctcgaa
     1741 agaacatttc aatccaaaca atgcctttga cgccgaaggt ttaaccaaac tgactcagga
     1801 gacggagcgc cgtcgtcgcg aacgtaacga agttgaacag gatgtagaag ttgcggtgcg
     1861 tgagaaaaac cgtgatgcac tttcgcgcaa gttggagatt gaacaacaag aagcgtttat
     1921 gacgcttgag caggagcagc aggttaaaac ccgtactgcc gaacagaatg cacgtattgc
     1981 ggcttttgaa gctgaacgtc gtcgtgaagc agagcagacg cgaattctgg ctgaacgaca
     2041 aattcaggaa acggagatcg agcgcgagca ggccgtccgc tcaagaaagg ttgaggctga
     2101 acgtgaagtt cgtattaaag agatcgaaca gcagcaggtc accgaaatcg ccaaccagac
     2161 gaaatcgatc gctattgccg ccaaatcgga acaacagtcc caggcagaag cgcgtgctaa
     2221 ccttgcactc gcagaagcag taagtgcgca acaaaacgta gaaaccactc gccagaccgc
     2281 agaagccgat cgtgctaaac aagttgccct aatcgctgcc gcgcaggatg cagaaaccaa
     2341 agcggttgaa ctgaccgtgc gggcgaaagc agaaaaagaa gccgcagaga tgcaggcggc
     2401 ggctatcgtt gagttagccg aagctacacg taaaaagggt ctggcggaag cagaagcaca
     2461 acgtgcgctg aacgatgcta tcaacgtact ttctgatgaa caaaccagcc ttaaattcaa
     2521 actggccttg ttgcaggcgc tacctgcggt aatagagaaa tccgttgagc cgatgaagtc
     2581 cattgacggt atcaagatta ttcaggtcga tggtctgaat cgtggcagcg ctgcgggtga
     2641 tgcaaacacg ggtaatgtgg ggggcggaaa cctagcggaa caagcattat cagccgctct
     2701 ctcttaccgc acacaggcac cgctgattga ctccttgctc aatgaaattg gcgtttcagg
     2761 cggctcactg gcggcattga cttcaccctt aacctcaaca actcccgtcg ccgaaaacgt
     2821 agaataaatt ttcagctatg aacgtccctg gcacaaaaag ccggggattt tttatatctg
     2881 cgttccgcta aaaactgcaa atgatcaggc cgttgcaacg ttttgcgtga ccgctcgggg
     2941 aaggcaaaac tgcctctggg aaagcgttgc gcgaggtccg gcgctcatca acaatcgggg
     3001 agtcagcaaa gggctgaaac gggaaagccc ctcccgagga aggggcctta cagaaggaaa
     3061 gggttatgat gaagctcgtc atcatactga ttgtgttgtt actcgtaagt ttcgcagctt
     3121 attaacagcc aatcagaggg gggagaaatc ctccctcacc cttgttcctt tactttaggt
     3181 tgaaaaaaaa tcagcgtcaa gaaggtttca ttaacacata tccctggccc aaaaagccgg
     3241 ggatttttta tatctgcgtt ccgctaaaag gtgcaaatgc tcaggacgtt gcagcgtttt
     3301 gcgtgatcgc tcggggaagg caaaactgct tcagggaaag cgttgcgcgg ggtccggcgc
     3361 tcatcaacaa tcggggagtc agcaaggggc tgaaacggga aagcccctcc cgaggaaggg
     3421 gccttataga aggaaagggt tatgatgaag ttcgtcatca tactggttgt gttgttactg
     3481 ttaagtttcc cgacttacta acaactgatc agagggggaa gaaatcctcc cttacccttg
     3541 ttcctttact ttaggttgaa aaaacaacag cgtcaatagg cctgcctgct acaaagcgag
     3601 atctgtgaac cgctttccgg ttagcctttt ttatcctgtt ggatcttctt gatgatgtta
     3661 gtggtcgagc aaccgtcttc gaagttgagc accagcactt cgccgccgtt ggcccagact
     3721 tctttactcc cggcgatctc ttctggttta tagtcgccgc ccttcaccag taaatctggc
     3781 aggatcccgg cgatcaagcg ctgcggcgtg tcttcttcaa acgacaccac ccagtcgacc
     3841 gcttccagtg cgcccagcac aatcatacgc tgttcgagcg ggttaacggg gcgggaatcc
     3901 cctttcagcc gtttggtgga ggcatcgctg ttcacagcga caatcagacg gtcacccagt
     3961 ttgcgagcat ttgccagata ggagacgtgc ccggcgtgca ggatgtcaaa gacaccgttg
     4021 gtcattacca ccttttcgcc gcgtttacgc gctgccgcta cggccagctt cagttcctct
     4081 tcggtcatca cgccaaagcc ggtatctgcg cgcccacgca ctgcattttc cagctcgatc
     4141 ggcgaaaccg tggaggttcc cagtttgccg accaccacgc cagccgccgc attggcaaag
     4201 aagcaggctt cttccagcga attacccgct gccagcgttg ccgccaggac gccaatcacc
     4261 gtgtcgcccg caccggtaac gtcatacact tcctgcgcct gggttggcat atgcagcggc
     4321 gctttacccg gttgcagcag cgacataccc tgttcggaac gggtcactaa cagagccgag
     4381 agttcgtaat cggcaatcag tttcatgccg cgctcaacaa tctcttcttc ggtcttacat
     4441 ttaccgacaa cagcttcaaa ttccgagaga ttcggcgtta acagcgtagc gccgcggtag
     4501 cgctcaaaat cggtaccttt tggatcaatc agcaccggaa cgcccgcttt acgcgccagt
     4561 tggatcatct gctgtacgct tgccagcgca cctttggcgt agtcagaaag caccagcgcg
     4621 ccaatcgaac tcagcgcctg attaatccgc tcgtgcagcg gctgcggatc aacgccttcg
     4681 aaaccttctt cgaaatccag acggatcagc tgttggttgc gggaaagtac ccgtaatttg
     4741 gtgatggtcg gatgcgtcgg tacagaaacg aagtcgcatt tgacgttgac gtcggccaga
     4801 gatttactca gcgcgcgcgc tgcatcgtca atgcccgtca acccgaccag gcgtgcatta
     4861 gcaccgagag aagcgatatt catcgccacg ttagccgcgc cgcccggacg ttcttcgatg
     4921 gtattcactt taaccacggg caccggcgct tccggcgaga tacggctgga gggaccgtac
     4981 cagtaacgat ccagcattac atcaccaacc accatcactc ctgcacgttc aaactctggc
     5041 agcgttactt tcattcctgt ctcctgagag attcaaaatt tgcgcgcgat aataccatac
     5101 ttcattcttc caccagccac ttctgccagc ttgcccgcac cagatcacgc tctgcggtga
     5161 agcaatcccc cgacacatgg cccggcaatt cctgtaatgc cagatgatga agttcatcgc
     5221 gcagcgtcgt gtaagcacgg gtcagcgcca tcgcttcctg ctcttccata atgtcgtttt
     5281 gcgccagcag ctccagaata cgcacgttgt ctgaccagcg cgttaacttc ggtttttcat
     5341 gagcgtagcg caacaccaga tattgggtaa taaattcgat atcggtaatt ccgccttcat
     5401 cagctttgat atcaaagcga tcgcgatgtt tattgccgag atgagcgcgc attttctcgc
     5461 gcatttcccg cacttccgtt tgcagagttc taccttcacg cggcagcgtc ataatctcgc
     5521 gacgcactgc gtcaaagtgc gcggtgagct gcggatcgcc gtacactacc cgcgcacgca
     5581 ccagcgcctg atgttcccac gtccaggcct cgtttttctg ataatcggca aatgcttctg
     5641 tggaggtaac cagcattccc gccgccccgg acgggcgcag ccgcgcatcc acttcgtaca
     5701 aaatgccgga agaggttcgc gtgctgaaca ggtgcataat gcgttgcgcc agacgaagat
     5761 aaaactgtcg tccgtcgatt tcccgctcgc cgtcagtcat cgcatccatt gggcaatcat
     5821 ggaggaagat caggtcaaga tcggaactgt agcctaactc ccagccgccc agcttgccgt
     5881 agccgaccac tgcaaaacca cgcccttcgc gatcgttgag gtgattcggc ttaccgtagc
     5941 gggcaaccat ttgaacccac gcctgctgaa cgacggcatc tatcatggct tccgccagcc
     6001 aggttaagtg atcgctcact ttcatcaccg gtagcgtacc agcgatatcc gctgcggcga
     6061 tgcgtaacag ctgcgcctgt ttgaactgac gcagcgcctc aagctgttgc tcttcgtcat
     6121 cttccggcac gcgcagcaaa tactggcgca actcatcgcg gtaggcatcg gtcgccgtcg
     6181 gctggtaaag agtgtttgga tcgagcaatt catccagcaa taatggataa cgcgccagct
     6241 ggctggcaat catcggcgat gcggcacaca gagaaatcaa atggttaagc gccgcaggga
     6301 attcactgag caattctaaa taggtggtgc gggtaacaat ccccaccagc aaggcggtaa
     6361 tgcgcgacag cgtgacagca gcgtcttcac gcgcacagac atcacttagc agatgcggca
     6421 tcagatggtc gagcacctga cgtcctcgcg gcccgatggt gcgcttatcc agctctttgc
     6481 ggaaatcgga aatcagcgtt agcacctgtt tgcgatcatc ctcgctaaga tgcgccagca
     6541 ctggcgtagt gtcatcttcc tgcaacgcat cctgccacag ctcacgccac tgttccgaca
     6601 gcgactcttc ctgagtttca ctttcgtcgt cgccaatcaa ttcattaaac acccggcgca
     6661 cattgttcat atgtgcggtc agcgccccgg tcagttgcgg ccagtcagca aagtccatcg
     6721 cccacgccag ccgcgcacgg ttaagctcat cagaaggaag cgtctgggtt tgttcgtcgt
     6781 taatgctttg cagcaggttt tccagacgcc gcaggaacag atacgccact cgcaattgtt
     6841 cagcatcgtt ttcagaaagc agatgcagcg cagcaatggc gctgagcgtt ggcagtaaag
     6901 agcacgattg cagcgacggt tcgcgcccgc cgcgaatgag ctggaatacc tggacgataa
     6961 attcaatttc gcgaatacca cccgcgccaa gtttgatgtt gtcggtcaaa ccgcgccgac
     7021 gcacttcacg ggcaatcatt cctttcatgt tgcgcagcga ctgaatcacg ctgaaatcga
     7081 tgtaacgacg gaaaacaaac gggcgcagca tcgcacgcaa ctcgttagca tagacgcctt
     7141 cgctatcgcc cataatccgc gctttgacca tcgcgtaacg ctcccagtcg cgcccctgct
     7201 cctggtaata atcctccaac gcggcaaagc tcagcaccag cgggccactt tcgccaaacg
     7261 gacgcagtcg catatccacg cgatagacga agccatcctg cgttggttga tccagcactt
     7321 taatcagccg ctgccccatg cgggtaaaaa actgcgcgtt atccagttcc cggcgtccgc
     7381 cctgcgtaca accatgttcc ggccaggcaa aaatcagatc gatatcagag gagaaattca
     7441 gttccccgcc gcccagctta cccatgccta agatcagcag cggttgcgct tcgccctgcg
     7501 cattgcacgg cgttccccac tcgcggcagc aggcgtcata cagccagtca cgcgccgcaa
     7561 caatcagcgt ctccgccaga taactgagct gctgcaatat gctctcttca gtaaccagtg
     7621 ccagcgtttg cgcccatgcg atgcgcacca taatgcgccg ccggaatagc cgcagctcgc
     7681 gcattaaccc ggcttcgtca ctcacattac tgagcgcctc ctgcaaccat gcagcgtaat
     7741 gctgccattc atcggcctgc ggcggttggc tttccagttc cgtcaaccac tctcgatgcg
     7801 caattatgct gtcctgcaca aaatcactga aagtaagtac tgacttcgcc tgtgcactta
     7861 gtggttcctc ggctaaaggc tctggcagcc gctcaacaac ggtctgccag tactgctgta
     7921 acggtgaaga gagcggcttc ataagcgatt ttatccttgc ctgattaacg ttttccgctg
     7981 tgcaaccaga acggttcctg attgtttgcc tcattacgga aatgctcaat ttcaatgcgt
     8041 tgcccggtcg caatagcgtg acgcagcccc tgccagttct ccagccaggc atgcgcgacg
     8101 acaggatcat agtaacccgc cagcaacagt attgagtcaa tgtcacgcgt caggcgtggc
     8161 aactggtcac ggtagagatc gcctaacggc tggcagaaaa cgcttttcag ttcagcggca
     8221 tggcgggaaa gatggatatc ggcaaagcgt ttgaaggagt cgctgatttt gccctgcgct
     8281 ttggcatcta aaaatggctg ccatgctttg cttaccaacc attcggtcaa cgccagcttc
     8341 gccattgccg tttcggtaga gtagaccgcc gtcacggcag aaaccgcaga agcaatggtc
     8401 gcttcgcatt gggtcagcaa atcacgtaag tgagtgctcg ctttacgcgg cacaatacca
     8461 ccgaacagca tcagcgtatg acggaccagg ctaatggctg ccagcacctg ttctttcgcc
     8521 gcatcgttac cgcgtaccca cagttcttca tgatactgcc attgcgctaa cgccagctcg
     8581 agcgccgctt ccagcccctg ttctacatcg gcttttgccg caacatgcaa aatggtggtc
     8641 ggtttgattt cacgcgccgg attgccctgc gccagatgat agccacgcgc cgctttgctc
     8701 aggctgccct ggcgtaatcc ggtttgcgat accagttggt tcgccagttt cagcaccgcg
     8761 cgcgtgtcgc cgctaagcag ttccagttcc agctcacaga taggttcagc aaattccccc
     8821 gctttcacct ccccctgatc gagagcgatt tcaattcggc tgccatcgac cgccaccagc
     8881 catttttcgc gataaaaatc ggtgctgaac agcggttgta cgcgggaagc gagatcggca
     8941 ggcaattcac cgttcggcca gacttccgag ggaaactgcg ccaggtcgag cgtcggttcg
     9001 ctcaacgcca cgttatattc cgggcgctgg tgtaagccgc ctgtcactct tcctgcaact
     9061 ttcatggtca tctcatagcg accgttttcg ccacgaatac gtaagcccat atcgtgccta
     9121 cgcagccagt tatccggcgt ttcgtagtaa atattcagca actgcacggg gtcatggtgc
     9181 tcgccgccca gcgtattgag atggtcacgc aacgcctcaa cggcactgtg attaacaata
     9241 aactttaatt cgatttcctg agccatggcc tttacttatc ggttatgtca cataagtgac
     9301 gatgaacggc gaacttaatg cgatcttttt tgtcagtaga tagtattttg cgccaaattg
     9361 ccatgcaacg ggcaatttga cgggcgtaaa agtttgaagc agtggcaaga cctgacacag
     9421 aggattccga ttgatgtcga atgctttgcg tagcgacact gatgccacta ctatcgttcc
     9481 actttccatg acaataacga cagcctgatg ccaaaattac gcctgatcgg attaacttta
     9541 ctcgcactta gcgcgactgc cgtctcacac gctgaagaaa cgcgctatgt ttctgacgaa
     9601 ctgaatacct gggtccgtag cggtccggga gatcattatc gcctcgtggg cacggttaac
     9661 gccggcgagg aagtgacctt attacaaact gacgccaaca ccaattatgc ccaggtgaaa
     9721 gacagctctg gccgtaccgc ctggatcccg ttgaaacaac ttagcactga gccaagcctg
     9781 cgctcccgtg tgccagatct ggaaaatcag gtcaaaaccc tgaccgataa actcaccaat
     9841 atcgataaca cctggaatca gcgcaccgca gaaatgcagc aaaaagtggc gcagagcgac
     9901 agcgtgatca acgggttaaa agaagaaaat cagaaactga aaaacgagct gattgtcgcg
     9961 cagaaaaagg tcgatgccgc cagcgtacag ctggatgaca aacagcgcac catcatcatg
    10021 caatggttta tgtatggcgg tggcgtgctg gggcttggct tgctgctcgg tctggtactg
    10081 ccgcacctga tcccaagccg caaacgcaaa gatcgctgga tgaactaaat cgccttcttc
    10141 gttgcactgt cttattatta ggaattgtgt atttgacgaa gggatgatgg cgtgaagatt
    10201 tatctggtcg gtggtgctat tcgggatgca ttgttagggc taccggtcaa agacagagat
    10261 tgggtggtag tcggcagtac gccacaggag atgctcgacg cgggctacca gcaggtaggc
    10321 cgcgattttc ctgtgtttct acatccgcaa acgcatgaag agtatgcgct ggcacgtacc
    10381 gaacggaaat ccggttccgg ttacaccgat tttacttgct atgccgcacc ggatgtcacg
    10441 ctggaagatg atcttaagcg tcgcgatctg accattaatg cgctggccca ggacgataac
    10501 ggcgagatta tcgacccgta caacggtctg ggcgatctgc aaaatcgtct gttgcgccat
    10561 gtttcccccg cttttggcga agatccgtta cgcgtactgc gcgtggcgcg ttttgctgcg
    10621 cgttatgccc acctcggttt tcgtattgcc gatgaaactc tgacgctgat gcgcgagatg
    10681 acccatgcgg gtgaactgga acacctgacg cctgaacggg tatggaaaga gacggaaagc
    10741 gccctcacca cccgcaatcc acaggtgttc ttccaggtac tgcgcgattg cggcgcactg
    10801 cgcgttttat tcccggaaat tgacgcactg tttggcgttc cggcccctgc caggtggcat
    10861 ccggaaatcg acacgggtat tcatacctta atgacgctct caatggcggc gatgctgagt
    10921 ccgcaggtcg atgtccgttt cgcaacttta tgccacgatc tcggtaaagg actgacgccg
    10981 ccagaacttt ggccgcgtca tcatggtcac ggtccggcgg gtgtgaaatt agtggaacaa
    11041 ctctgccagc gtctgcgcgt gccaaatgaa attcgcgatt tagccagact ggtggctgag
    11101 tttcacgatc tcatccacac cttcccaatg ctgaacccaa aaactatcgt caaattgttt
    11161 gattccatcg acgcctggcg taaaccgcag cgtgtcgagc aactggcgct gaccagcgag
    11221 gctgacgtac gcggcagaac cggttttgaa tcagcggact acccgcaagg ccgttggttg
    11281 cgcgaagcct gggaagtggc gcagtcagtg ccgacaaaag cggtcgttga agcgggattt
    11341 aaaggtgtgg agattcgcga ggagctgacc cgacggcgga ttgcggcggt agccagttgg
    11401 aaggaacaac gttgcccaaa gcctgactga gtataaaact aaacgttaac acgaaccatc
    11461 ccctcgcccc ttcggggaga gggttagggt gaggggaaca gaccagcacc ggtgcgaaca
    11521 atcaccccca ccccggccct ctccctacag agcgagggag aaaaccaggc ctgctcccga
    11581 accttttctc aatcaaaaga acacgacgta caccgcagcc gccacaataa agcgataaat
    11641 ggcgaacggg ataaacgaaa tgcgcttaat caattgcagg aaggttttaa tcgctatcag
    11701 cgccaccaca aaagcggtga taaacccaac ggcaaacatc gagatatcgc cggttgtcag
    11761 gaagccccag cttttgtaga gatcgagcgc cgttgcgccc atcatcatcg gtaccgccag
    11821 caggaacgag aactcggaag cagcgtaacg gctcacgccc atcagcatcc cacctgaaat
    11881 ggtggccccg gaacgggaga aacccggcca cagcgccaga cactggaaac agccaatcat
    11941 aaacgcctga cgataggtca tatcatcaag acccggcgca cgcggctctt tcggcttcag
    12001 gcattcggcg gcaatcagca acaaaccgcc aacgaccagt gcatacatca catttatcgg
    12061 gttaaacaat gacttaatcg tgtcgtggaa caacagcccc agtaccaccg ccggaatcat
    12121 ccccagcaaa atgtggatca gcgttaaacg acctttgctt tctccttcgt gctgcaacgg
    12181 gcggccaaag tggatgccaa tcaggccaaa cagacgccgc cagaacatca ctactaccgc
    12241 cagaattgat cctaactgga tcacaacttc aaaggttttc gccgtgtcgc cctcgaaccc
    12301 caacaagtga ccgacaataa tcatatggcc cgtgctggat accggcagaa attctgtcaa
    12361 tccttcgacc acacccaata ttgccgctat cagcagcgag tgcatatcgc tcatcaataa
    12421 acccctaaat tattaaaatg taccgcttgt ccggactact gcagtaagac caggttataa
    12481 ccgtttggtt taacagctgt aaaattaatt attttctttc agattattgc cacgctcaat
    12541 gattacacca acattcgccg cccgcgccat tgcgcctggc ttgctgagtt tgatgcgcac
    12601 ccacggcgag ttgaagcgtg ctaacagcag ctccgccacc tcttcagcca cgcgttccac
    12661 cagcgcaaaa cgcgccccct cgacgtggct gaccaccgtt tctgcaatgt cagcgtaact
    12721 gaggcaatcc gccacatcat cacttttcgc cgctttacgg ttatcccatg ccatttcgat
    12781 atcgaacact aacttctgtt cgatggtctg ttcccagtcg taaacaccaa tagtggtgat
    12841 taccgaaagt tgctctataa atacaatatc catcacgtcc tgcctgcttt ttggctaacc
    12901 ggataccact tccggcgaaa tgtgcgtatt atccacagat tcatcgttga acacgaattt
    12961 tcaaaacgga acagcttatg agtgcaatcg cgcctggaat gatcctcatc gcgtacctct
    13021 gcggctccat ttccagtgcc attctggttt gccgcttgtg tgggctgccc gatccgcgaa
    13081 ccagcggctc cggcaatcca ggcgcaacca atgtgttacg tatcggtggc aagggagcag
    13141 ccgtagcagt actgattttc gacgttctga aaggaatgtt gcccgtctgg ggcgcgtatg
    13201 aattaggtgt cagccccttc tggttaggct taattgccat cgccgcctgt cttggacaca
    13261 tctggcccgt tttcttcgga tttaaaggag gaaaaggcgt tgctaccgct tttggtgcca
    13321 tcgcacccat tggctgggat ctcaccggag taatggcggg aacctggtta ctgaccgtgc
    13381 tattgagcgg atactcgtcg ctgggagcga ttgtcagtgc actgattgct ccgttttatg
    13441 tctggtggtt taagccacaa ttcaccttcc cggtttcgat gctctcttgc ctgatcctgc
    13501 tgcgtcatca tgacaacatc cagcgtctgt ggcgtcgtca ggagacaaaa atctggacga
    13561 aattcaaaag aaagcgcgaa aaggatcccg agtgatttct ggtggatcta catgacctga
    13621 tagccttcat cgggcttgcc cagccattgc tggcaccatg ccgccagaaa ttccacgcag
    13681 acacgtaatt tcatgctgcg atagagcggc tcccggtaaa cagcccagat attggcgctt
    13741 tgtgcatact ccggcaatac ttgcaccagt ttgccactct ccagaaacgg caacacatcc
    13801 cactcggaac gcagcataat ccctttgccc tccagcgccc attgcaagac tatctcgccg
    13861 ctattggagg aaagatgccc gctaaccttc accgattttt tctcctgacc gttccccaac
    13921 tcccatattc cgtgggtcat atcgcgctct ttggtcacca gacagtcatg acgacttaat
    13981 tcctgtaatg attgtggctg cggatatttt tgcagatact caggtgctgc gcataatatt
    14041 cttttatttt tcgttaacag atgcgcaata taataatcag gaatttcgtc attaatacga
    14101 atatccagat caatattatc ctgcactaaa tcaatttgcc gatcgaacaa ttcaaaatgc
    14161 acctgtaact caggataatt gcgcataagt tcggtaatgg ctggcgcaat atggctgcgc
    14221 ccaaaaccaa aactgcaacc aatacgaatc atcccctccg ggcgcgtttt gatttgcgtg
    14281 acgtcatcga ccagtcgctg atattgggtg aggatttcca gcgcatgttc gtaacaacgt
    14341 tggccacttt cagtcagcgc cacgccgcga gcggagcggt tcagcagcgt tgttgccagc
    14401 gtattttcaa ggatttggat ccgcttggtg acaaatgcag gcgtctgccc aagcgtcgcc
    14461 gctgcggcgc taaaactccc gctgtggacg atttctacca gtacctgcaa gtctttggct
    14521 aaaggccagc tattcagcat cgttaattat ccgcagttgt gataagcgca gtgtatttcg
    14581 caaaacattg ccattccctg ctctggcgca atattcattc ttattttcgc gttgattcac
    14641 gaattgttaa ttacatattc acggtggcaa aaaatataaa accacatttt tagtggtagt
    14701 ttgtggcggt gaatttttcc agacaaatac aaaaactgga gttgccatga tgagcgaaag
    14761 taataagcaa caggcagtga ataagttgac ggagattgtc gctaacttta ccgccatgat
    14821 ttctacccga atgcctgatg acgtggtgga taaactaaaa cagctaaagg atgccgaaac
    14881 gtcgtcgatg gggaaaatta tctaccatac gatgttcgac aacatgcaaa aagcgattga
    14941 cctgaatcgt cctgcctgtc aggacaccgg ggagattatg ttcttcgtta aagtcggttc
    15001 ccgcttccca ctgcttggcg agctgcaaag catactcaaa caagccgtgg aagaggcaac
    15061 cgtcaaagcg ccactacgtc acaatgcggt agaaattttt gacgaagtaa acaccggcaa
    15121 aaataccggt agcggcgtac cgtgggtcac ctgggacatc atccccgaca atgacgatgc
    15181 ggaaatcgaa gtttacatgg caggcggcgg ctgcacgcta cctggccgct cgaaagtgtt
    15241 aatgccgtca gaaggctacg aaggcgtggt gaaattcgtc ttcgaaaata tctccaccct
    15301 cgccgtaaac gcctgtccac cggtactggt gggcgtgggc atcgccacct cggtggaaac
    15361 cgccgccgta ctctcgcgta aagccatttt gcgcccgatt ggcagccgcc atcccaatcc
    15421 aaaagcggca gaactggagc tacgcctgga agaaggactc aaccgtctgg ggattggtcc
    15481 acaagggctg actggcaaca gttcagtgat gggcgtacat atcgaatctg ccgcccgcca
    15541 tccgtcaacc atcggcgttg ctgtctctac cggctgctgg gcgcatcgtc gcggcacact
    15601 gctggttcat gccgatctca cctttgaaaa tctgtctcac acccggagcg cgttatgaaa
    15661 aagatcctga caaccccgat caaagctgaa gatctgcaag atattcgcgt cgacgatgtg
    15721 atctacctga ccggtacgct ggtgacctgc cgtgacgttt gtcaccgccg cttgatcgaa
    15781 ctgaaacgtc cgatccctta cgatctcaac ggcaaagcga ttttccacgc tggccccatc
    15841 gtgcgcaaaa acggcgataa atgggagatg gtttccgttg gcccgacaac cagtatgcgt
    15901 atggaaagtt ttgaacgtga atttatcgag cagaccggcg tgaaactggt ggttggcaaa
    15961 ggcggaatgg ggccgctgac cgaagaaggc tgccagaaat tcaaggcgct acatgtgatt
    16021 ttcccggcag gctgcgcggt gctggcggca acccaggtgg aagagattga agaagtgcac
    16081 tggacagagc tcggaatgcc ggagtcgctg tgggtctgtc gggtcaaaga gttcggcccg
    16141 ctgattgtct ctattgatac ccacggcaac aacctgatag ccgaaaacaa aaagctgttc
    16201 gccgaacgcc gcgatcccat cgtggaagag atctgcgagc acgtccatta catcaaataa
    16261 ccctcccgga gaggctcgcc ccctctcctt tttcgcaggc ataacacgat gaaaccttcc
    16321 actgaatggt ggcgatacct tgcgccgctg gcggtcatcg ccattattgc tctaattccg
    16381 gttcccgcag ggctggagag tcatacctgg ctctactttg ccgtttttac tggcgtgatc
    16441 gttggactga tcctcgaacc cgtgccgggt gccgtggtgg cgatggtggg tatatccatc
    16501 atcgccatac tctctccctg gctgctgttc agcccggagc agctcgctca gccaggcttt
    16561 aaattcactg caaaatccct ctcgtgggcc gtttccggtt tttctaattc ggttatctgg
    16621 ctgattttcg ccgcctttat gtttggcaca ggctatgaaa aaaccgggct tggacgccgc
    16681 attgcgctga ttctggtgaa aaagatggga catcgcacgc tgtttctcgg ctatgcggtg
    16741 atgttctccg agctgatcct cgcacctgta acaccgtcca actcggcgcg tggtgcgggg
    16801 attatctatc ccatcatccg taacctgcca ccgctctatc aatcacaacc aaacgacagc
    16861 agttcgcgca gcattggctc gtacatcatg tggatgggga ttgttgccga ctgcgtgacc
    16921 agcgccattt tcttgacggc gatggcacca aacttgctgt taattggact gatgaaaagc
    16981 gcatctcacg ccacgctgag ttggggcgac tggttcctcg ggatgttgcc gctcagtatt
    17041 ttgctggtgc tgctggttcc ctggctggct tatgtgctgt acccgccggt actgaagtct
    17101 ggcgatcagg tgccgcgctg ggcagagacg gaactgcagg caatgggccc gctctgttcg
    17161 cgtgaaaaac ggatgctggg gctgatagta ggcgcgctgg tgctgtggat tttcggcggt
    17221 gattatatcg atgccgcgat ggtcggttac agcgtggtgg cactgatgct gcttctgcgc
    17281 attatcagtt gggacgacat tgtcagtaat aaagcggcgt ggaacgtttt cttctggctg
    17341 gcctcgctta tcaccctcgc taccggactc aacaacaccg gttttattag ctggtttggc
    17401 aaactgttag caggcagctt aagcggttat tcgccaacga tggtgatggt ggcgttgatt
    17461 gtggtgtttt atctgctgcg ctactttttc gccagcgcca cggcgtatac ctccgccctc
    17521 gcaccgatga tgattgccgc tgcgctggcg atgccggaaa tcccgctgcc ggtgttctgc
    17581 ctgatggttg gcgcggcaat tggtctgggg agcattctta caccatacgc caccggaccc
    17641 agtccgattt actacggtag tggttatctg ccaacggcgg attactggcg actgggggcg
    17701 atttttgggc tgatattcct cgtgttgctg gtgattaccg gcttactgtg gatgcccgtg
    17761 gtgttgcttt aagtgaggac ggtgccggaa attgctctcc ggcacccaat agttttacgc
    17821 agccggtaac tccgccagcg gccagcgcgg acgcacgcta acgccgagat ccgccgtcgc
    17881 gcctgcttta aaccgcacca ttccggcata ggcgatcatc gcgccgttat cagtacaaaa
    17941 ctccggacgc gcgtagaaca cttcgccgcg gcgttttttc atcatttcag ccagcttcgc
    18001 ccgtaacgtg cggttagcac tcacgccgcc cgccatgacc agtcgcttaa agcccgtctg
    18061 atccaacgct cgcttacact taatcatcaa cgtatcgacc accgcatctt caaaggcgcg
    18121 ggcgatatca gcacgcgtct ggtcgtcggt gccgttgtca cgaatcgtgt tcgccgcaaa
    18181 ggttttcaga ccagaaaagc tgaaatccag ccccggacgg tcggtcatcg gacgcgggaa
    18241 aacaaagcgc ccggcagtac cctgagccgc cattttcgac agtaacggtc cgccaggata
    18301 atccagcccc agcagcttcg cggttttatc aaacgcttca ccggcggcat catcgataga
    18361 ctcgccgagc agctcgtact gaccaatgcc agtcacgctg attaactgcg tatgaccgcc
    18421 ggacaccagc agcgcgacaa acggaaattc cggcgggtta tcttccagca tcggcgctaa
    18481 cagatgccct tccatatggt gtaccgggat tgccggaacg ttccaggcaa acgccagaga
    18541 acgccccacg gttgcgccaa ccagcagtgc gccgactaat ccagggcctg cggtataggc
    18601 cacagcatca atgtcttttg ccgttaagcc agactccttt agcgccgcct ggatcaacgg
    18661 tacggttttg cgcacgtgat cgcgggaggc cagttcaggc acgacgccgc catagtcagc
    18721 gtgcaatttc acctgactat acaattggtt ggctaacaaa cctttttcat cgtcgtaaat
    18781 ggcgatgccg gtttcatcgc aggaagtttc aatacccagt acacgcatga cttgttttac
    18841 ctcgctttat taccgcgcag tgtaggacca atgcgggttg atgtaaaact ttgttcgccc
    18901 ctggagaaag cctcgtgtat actcctcacc cttataaaag tccctttcaa aaaggccgcg
    18961 gtgctttaca aagcagcagc aattgcagta aaattccgca ccattttgaa ataagctggc
    19021 gttgatgcca gcggcaaacc gaattaatca aaggtgagag gcacatgccg gtaattaaag
    19081 tacgtgaaaa cgagccgttc gacgtagctc tgcgtcgctt caagcgttcc tgcgaaaaag
    19141 caggtgttct ggcggaagtt cgtcgtcgtg agttctatga aaaaccgact accgaacgta
    19201 agcgcgctaa agcttctgca gtgaaacgtc acgcgaagaa actggctcgc gaaaacgcac
    19261 gccgcactcg tctgtactaa ttccccgaga gcgttgctct ccgatcagac cgagttgtag
    19321 ttgtaaggcc gtgcttccga aaggaatgcg cggcttattt tcgtttatga attgctaaaa
    19381 atcggggcct atggctggac gaatcccacg cgtattcatt aatgatctgc tggcacgcac
    19441 tgacatcgtc gatctgatcg atgcccgtgt gaagctgaaa aagcagggca agaatttcca
    19501 cgcgtgttgt ccattccaca acgagaaaac cccgtcattc accgttaacg gtgagaaaca
    19561 gttttaccac tgctttggat gtggcgcgca cggcaacgcg atcgacttcc tgatgaacta
    19621 cgacaagctc gagttcgttg aaacggtcga agagctggca gcaatgcaca atcttgaagt
    19681 gccatttgaa gcaggcagcg gccccagcca gatcgagcgc catcagaggc aaacgcttta
    19741 tcagttgatg gacggtctga atacgtttta ccaacaatct ttacaacaac ctgttgccac
    19801 gtctgcgcgc cagtatctgg aaaaacgcgg attaagccac gaggttatcg ctcgctttgc
    19861 gattggtttt gcgccccccg gctgggacaa cgtcctgaag cggtttggcg gcaatccaga
    19921 aaatcgccag tcattgattg atgcggggat gttggtcact aacgatcagg gacgcagtta
    19981 cgatcgtttc cgcgagcggg tgatgttccc cattcgcgat aaacgcggtc gggtgattgg
    20041 ttttggcggg cgcgtgctgg gcaacgatac ccccaaatac ctgaactcgc cggaaacaga
    20101 cattttccat aaaggccgcc agctttacgg tctttatgaa gcgcagcagg ataacgctga
    20161 acccaatcgt ctgcttgtgg tcgaaggcta tatggacgtg gtggcgctgg cgcaatacgg
    20221 cattaattac gccgttgcgt cgttaggtac gtcaaccacc gccgatcaca tacaactgtt
    20281 gttccgcgcg accaacaatg tcatttgctg ttatgacggc gaccgtgcag gccgcgatgc
    20341 cgcatggcga gcgctggaaa cggcgctgcc ttacatgaca gacggccgtc agctacgctt
    20401 tatgtttttg ccggatggcg aagaccctga cacgctggta cgaaaagaag gtaaagaagc
    20461 gtttgaagcg cggatggagc aggcgatgcc gctttccgca tttctgttta acagcctgat
    20521 gccgcaagtt gatctgagta cccctgacgg gcgcgcacgt ttgagtacgc tggcactgcc
    20581 attgatatcg caagtgccgg gcgaaacgct gcgaatatat cttcgtcagg aattaggcaa
    20641 caaattaggc atacttgatg acagccagct tgaacgatta atgccaaaag cggcagagag
    20701 cggcgtttct cgccctgttc cgcagctaaa acgcacgacc atgcgtatac ttatagggtt
    20761 gctggtgcaa aatccagaat tagcgacgtt ggtcccgccg cttgagaatc tggatgaaaa
    20821 taagctccct ggacttggct tattcagaga actggtcaac acttgtctct cccagccagg
    20881 tctgaccacc gggcaacttt tagagcacta tcgtggtaca aataatgctg ccacccttga
    20941 aaaactgtcg atgtgggacg atatagcaga taagaatatt gctgagcaaa ccttcaccga
    21001 ctcactcaac catatgtttg attcgctgct tgaactgcgc caggaagagt taatcgctcg
    21061 tgagcgcacg catggtttaa gcaacgaaga acgcctggag ctctggacat taaaccagga
    21121 gctggcgaaa aagtgattta acggcttaag tgccgaagag cgatcgggaa gcccccgaca
    21181 gccgcactga gaggcagcgg caaatatata agtacgccct cgtaattatc gttggcggta
    21241 aacaaccgtt ggatttcagc gttaacggct gaaggacatc gggtcaatcg cccaacacca
    21301 acctcatgaa ataagtgtgg ataccgtctt atggagcaaa acccgcagtc acagctgaaa
    21361 cttcttgtca cccgtggtaa ggagcaaggc tatctgacct atgccgaggt caatgaccat
    21421 ctgccggaag atatcgtcga ttcagatcag atcgaagaca tcatccaaat gatcaacgac
    21481 atgggcattc aggtgatgga agaagcaccg gatgccgatg atctgatgct ggctgaaaac
    21541 accgcggacg aagatgctgc cgaagccgcc gcgcaggtgc tttccagcgt ggaatctgaa
    21601 atcgggcgca cgactgaccc ggtacgcatg tacatgcgtg aaatgggcac cgttgaactg
    21661 ttgacccgcg aaggcgaaat tgacatcgct aagcgtattg aagacgggat caaccaggtt
    21721 caatgctccg ttgctgaata tccggaagcg atcacctatc tgctggaaca gtacgatcgt
    21781 gttgaagcag aagaagcgcg tctgtccgat ctgatcaccg gctttgttga cccgaacgca
    21841 gaagaagatc tggcacctac cgccactcac gtcggttctg agctttccca ggaagatctg
    21901 gacgatgacg aagatgaaga cgaagaagat ggcgatgacg acagcgccga tgatgacaac
    21961 agcatcgacc cggaactggc tcgcgaaaaa tttgcggaac tacgcgctca gtacgttgta
    22021 acgcgtgaca ccatcaaagc gaaaggtcgc agtcacgctg ccgctcagga agagatcctg
    22081 aaactgtctg aagtattcaa acagttccgc ctggtgccga agcagtttga ctacctggtc
    22141 aacagcatgc gcgtcatgat ggaccgcgtt cgtacgcaag aacgtctgat catgaagctc
    22201 tgcgttgagc agtgcaaaat gccgaagaaa aacttcatta ccctgtttac cggcaacgaa
    22261 accagcgata cctggttcaa cgcggcaatt gcgatgaaca agccgtggtc ggaaaaactg
    22321 cacgatgtct ctgaagaagt gcatcgcgcc ctgcagaaac tgcagcagat tgaagaagaa
    22381 accggcctga ccatcgagca ggttaaagat atcaaccgtc gtatgtccat cggtgaagcg
    22441 aaagcccgcc gtgcgaagaa agagatggtt gaagcgaact tacgtctggt tatttctatc
    22501 gccaagaaat acaccaaccg tggcttgcag ttccttgacc tgattcagga aggcaacatc
    22561 ggtctgatga aagcggttga taaattcgaa taccgccgtg gttacaagtt ctccacctac
    22621 gcaacctggt ggatccgtca ggcgatcacc cgttctatcg cggatcaggc gcgcaccatc
    22681 cgtattccgg tgcatatgat tgagactatc aacaaactca accgtatttc tcgccagatg
    22741 ctgcaagaga tgggccgtga accgacgccg gaagaactgg ctgaacgtat gctgatgccg
    22801 gaagacaaga tccgcaaagt gctgaagatc gccaaagagc caatctccat ggaaacgccg
    22861 atcggtgatg atgaagattc gcatctgggg gatttcatcg aggataccac cctcgagctg
    22921 ccgctggatt ctgcgaccac cgaaagcctg cgtgcggcaa cgcacgacgt gctggctggc
    22981 ctgaccgcgc gtgaagcgaa agttctgcgt atgcgtttcg gtatcgatat gaacaccgac
    23041 cacacgctgg aagaagtggg taaacagttc gacgttaccc gcgaacgtat ccgtcagatc
    23101 gaagcgaagg cgctgcgcaa actgcgtcac ccgagccgtt ctgaagtgtt gcgtagcttc
    23161 ctggacgatt aatcggtagg ccggatcagg cgttacgccg cacccggcac tagtccctct
    23221 gcacaaacgc cacctttt
//
LOCUS       DAKISA010000070        23222 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.070, whole genome shotgun sequence.
ACCESSION   DAKISA010000070 DAKISA010000000
VERSION     DAKISA010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23222
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.070"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(163..978)
                     /locus_tag="OVW45_002737"
     CDS             complement(163..978)
                     /locus_tag="OVW45_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5527144.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFINSVRTDC
                     KCILLEHHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            complement(1221..2270)
                     /gene="yahK"
                     /locus_tag="OVW45_002738"
     CDS             complement(1221..2270)
                     /gene="yahK"
                     /locus_tag="OVW45_002738"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HCV5527145.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMIGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAEKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIADSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(2647..4029)
                     /locus_tag="OVW45_002739"
     CDS             complement(2647..4029)
                     /locus_tag="OVW45_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCV5527146.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTYVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(4039..4989)
                     /locus_tag="OVW45_002740"
     CDS             complement(4039..4989)
                     /locus_tag="OVW45_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HCV5527147.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTDVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFSPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(5132..6550)
                     /locus_tag="OVW45_002741"
     CDS             complement(5132..6550)
                     /locus_tag="OVW45_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HCV5527148.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(6550..8097)
                     /gene="fdrA"
                     /locus_tag="OVW45_002742"
     CDS             complement(6550..8097)
                     /gene="fdrA"
                     /locus_tag="OVW45_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HCV5527149.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPTLEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(8087..8950)
                     /locus_tag="OVW45_002743"
     CDS             complement(8087..8950)
                     /locus_tag="OVW45_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HCV5527150.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQTVNDKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPAEKYKEEFYRGLLRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYGVPGNSTQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(8990..9595)
                     /locus_tag="OVW45_002744"
     CDS             complement(8990..9595)
                     /locus_tag="OVW45_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HCV5527151.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            9853..10350
                     /locus_tag="OVW45_002745"
     CDS             9853..10350
                     /locus_tag="OVW45_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCV5527152.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALTAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            10442..11374
                     /locus_tag="OVW45_002746"
     CDS             10442..11374
                     /locus_tag="OVW45_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCV5527153.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(11416..12504)
                     /locus_tag="OVW45_002747"
     CDS             complement(11416..12504)
                     /locus_tag="OVW45_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCV5527154.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(12646..16629)
                     /gene="ehaA"
                     /locus_tag="OVW45_002748"
     CDS             complement(12646..16629)
                     /gene="ehaA"
                     /locus_tag="OVW45_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HCV5527155.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKTIDFGKENQSIDYRITVTDNANLVINATDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDRGYFNVSNGGKIH
                     VKGTSRLTYLQGNVSGEGSQVNSKTFFMGVYGSYGGNQYLSVNNGGEVNASEKISLGY
                     YDQNSDTTLAVSEGGKISAPTISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SEKKITLNHTDKNATISADIVSDSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITQNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALTGNNYAFSGYID
                     VASGAVAVISEDKNIGRADLDVDGKLQINANKDWVFDNDLQGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQRGGITAGQGSLVTVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVRSVSCDIDSALSLTEVDD
                     GNSAIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSAEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TYYTDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     SWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
     gene            complement(17202..19154)
                     /gene="betT"
                     /locus_tag="OVW45_002749"
                     /pseudo
     CDS             complement(17202..19154)
                     /gene="betT"
                     /locus_tag="OVW45_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
     gene            19293..19880
                     /gene="betI"
                     /locus_tag="OVW45_002750"
     CDS             19293..19880
                     /gene="betI"
                     /locus_tag="OVW45_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HCV5527156.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVAGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPREQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            19894..21366
                     /gene="betB"
                     /locus_tag="OVW45_002751"
     CDS             19894..21366
                     /gene="betB"
                     /locus_tag="OVW45_002751"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HCV5527157.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTNYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            21380..23050
                     /gene="betA"
                     /locus_tag="OVW45_002752"
     CDS             21380..23050
                     /gene="betA"
                     /locus_tag="OVW45_002752"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HCV5527158.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPTLDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
BASE COUNT         5540 a         6270 c         5683 g         5729 t
ORIGIN      
        1 ctgctggtgc ttttttaagc gaagtgtcgc cgatcacaac aaaatatcgg cgaaaaggcg
       61 gtggaatgcc atttggcata cggatataca gatgaaatga ttacctgctt tgcgattcac
      121 tctctggtgg aataacaact tgcaggtgga accctttctg aactaaagag tgtagcggat
      181 tttaacgtgc ttatttacag gataatatct gtcacctaat tcctgcactc tattagatat
      241 ccattcattt aatcgtgcat catctctgta tgtttttacg tccagaacag agagcaataa
      301 catcaccagt gattcaggaa aaacagggta gatatttttt aagtgtaata ataatccatg
      361 cgcaagcgcc tgcttaatgg gcgcgtctgt ctccggaata gacgacattt cgcgaagcca
      421 tttttccaca tgcatacact ctttcccttt agggaaaagt ctcagcaaag gcatatggtg
      481 ttcaagaaga atacatttgc aatctgttcg cactgaattg ataaactgca ctaatttctt
      541 aagcccggta aagcgtccag cctgcacatt tgctttaata tcaagaaacg catcatcctg
      601 gtcatatacc caaactgtcc ctttttgatt ttcaagtatg cgaaaaacaa acttaaccgg
      661 gtgatcaggg taatccatcc agtcataaaa tcctgggaaa tcattaaact gcacatttat
      721 ggtgagtgcg ttatcgtctg accactcaca gtctcgtacg gcagccatga gatgaatgag
      781 tttgtcgtca tcgactaaat catcacgaca atcataagga taatcataat attgtttaat
      841 caaaaccttg aatatatcga cacaggaaac cagggagctt ttgatatcct ctggtaactg
      901 attgttttta accgtattaa gtatgctttg ttgcgtatat ggcatcaaat tattgtgcgg
      961 agcttttaat gatatcatcg cgatctctct taaataaaaa taaacgtcgc agataataaa
     1021 gcgctgatta aaccaggcac tatcagaaat cgctcataac taacgtgaga agtattgtgt
     1081 actggaggga gttaatttaa caatttgaat ttacgttata ggttttactg ctactttgat
     1141 attatttcgc tctccttaac taaaaagtta agtggagagc gaagttaata tgttaaacca
     1201 cagcgtattt attaattttt tcaatctgtt agtgtgcgat tatcgataac aaaacgatat
     1261 ttcacatcac cacgcagcat tcgctcatag gcttcattaa tttgatcggc ccgaatcatc
     1321 tctatatcag ccacgatgcc atgttcggcg caaaaatcga gcatctcctg agtttctgga
     1381 atgccgccaa tcatagaatc ggctatcgca cggcgtttca tgatcaggtt gaaaacttcc
     1441 ggcgatttat gcggtgtcgc aggcgcacca accagcgtca tggtgccatc acgcttcagc
     1501 aaggtggtaa aatcgtcgag attatgtggc gcagctactg tattcaaaat gaaatcgaaa
     1561 ctcttcagat gagccgccat ctcatcggca ttgcgtgagt taacaacttc atcggccccc
     1621 agggcttttt ccgcttcgcg ttttgcctca gaagtggtaa atgccaccac atgtgccccc
     1681 atcgcgtggg ccagcttaat ccccatatgt cccagaccgc cgatgccgac cacgcccact
     1741 tttttacccg gcccggcctg ccagtgacgt agcggcgagt acgtggtgat ccctgcacac
     1801 aacaaaggag ccaccgccgc cagctgctct tgcgggtgac gaatacgcag aacatatcgc
     1861 tcatgaacga cgatctgttg tgagtagccg cccagagtat ggcccggttc gtccggcgtc
     1921 ggcgagttat aggtgccgat catgtgatca cagtagtttt ccagcccgtc ctcacactct
     1981 tcgcaatgtt tacaactgtc gacaatgcag ccgacaccga ccagatcgcc cggcgcatgt
     2041 ttttctacct gatcaccaac ggctaccaca cgccccacaa tttcatgacc cggtacgcag
     2101 gggtaaaccg tccccgccca ctcggaacgg acctggtgga gatcggaatg gcagacgcca
     2161 cagtaagcga tttcgatttt gacatcattc ggtcccggtt cacgccgtgt gatatccatc
     2221 ggttcaagtg gttgtttagc ggaatatgca ccaacagctt tgatcttcat tgtgtttact
     2281 cctgattagc tatgtgtatt tggcgacgcc tgatatgtca tcgcggcaaa acgcgtccat
     2341 tgaatatagc caatatttaa accacccgat ccagccccaa catgccgcga acctgaatga
     2401 aacagagcag caacgttccc cataaggcga gcgcctataa gtgtgatatt tgctatccgt
     2461 aaaagactaa aatcggcaaa actgatgatc ttttcacgta gacaaatagt tatctacaca
     2521 acgtcaataa gggcatattc ctcaaagata atgtccattt ctgccgacac tatcatattg
     2581 aggtcgcccc actccctggg gcttaaatct cttacaattc accgttgcag gcgtttcaaa
     2641 tgcgcatcaa cctgccacac tcccccacac cagttgccct ttatggaacg ttgcggttct
     2701 cggcgagata cgcgccaccg cctcggcgga acaggaggcg tccaccagca caaagctggc
     2761 atcatcctgc gcttttggcc atacacgctc gcctttttca ttcagcggca atacatcgcc
     2821 agtggctaga aacagcgaac gggagaggtt ctgttcgtta ggacgaatat agagctgcgc
     2881 gtagagattg gctttttcca gcatgtcgcc cagaccataa ggcgaccagt ggtcgataac
     2941 gctgtcagtg ccagtcatca cttttacgcc tttgtcgtgc aactgtttga gcggcatatg
     3001 cagcgtgcca atcggcaccg tcgaggcgat agaaatttgc tgcgccgcca tccgattcgc
     3061 cagcgcatcg acctgttgtt cgttgagcgt agccagcgca aaggcgtggc tgatggtcag
     3121 cttacctttc agttgcggcg ttttctctac cgtttcaacc atataattga tggctgccac
     3181 gcccgacgga gtggtttcgt gcaggtgaat atcgacacct ttgtcgtagt ccagcgcaat
     3241 ctgaaacatg gtgtcgaggg atttttccat cgcgccatcg acactggtcg ggtccagccc
     3301 gccgacgtaa tgcgcacccg cctgcatcgc ttcgcgcatt aagggttccg atttcgacag
     3361 cagcaaaccg tgctgcggga aggcgacgat ttcacactcg aagcccgcct gacgccgcgc
     3421 cagcaccgcc tgcaaatttt gcagattttt taggccggaa accggttcga tattgcagtg
     3481 gctgcgggca atggtggtgc ctttcgactg caatagatcg atcagttttt ccgcccgctc
     3541 ctgcgtatac ggttgtaact ccggcagcat tttctgctcg agtttgatca tgtcctggat
     3601 agtggtgcct gccggacgat tgagcgagcg ccacggcccg ccgtagaagg ttttgtcgag
     3661 atgaatatgc atgtcgcgag tggtgggtag catcaactta ccgccagcgt catagtgcgg
     3721 cagcgtggcg tccggatgct gcttgttctc gcgcagggcg acaattttgc cgtcctgaat
     3781 ctccacggtc tggcgcgccg tgcgggtctg caccgccaca ccgttttcgt agtcaaaacc
     3841 ggtttccagc agcacgttat cgagatagta atgcgggtca gtgattttca tggtgttgtt
     3901 cgcttcgcag tttagggtgc cagctaccgc cgcatgggcg agcggcacat aggtaccaaa
     3961 cagcgcggcg gcggtgacca tcttaccgct ctggctcaga aattcacggc ggctattgct
     4021 ttctttcatc ctacgtcctt acgttttaat aatatgggtg cccgtttcac cgcgcagcgc
     4081 cgcaggcagg cattccggcg tggtgataat cacttctttg ccgccctgct ccaggaacgt
     4141 caggctggcg atgatttttg gcaacatgct gccgggagaa aaatgccctt cctgcatata
     4201 gcgagtcatg gtggcaatat ccacccgatc cagcgcctgc tgctgcggtt taccaaagtg
     4261 gatacagact ttttcgacgt cggtggtgat caccagaatg tcggcgtgaa tttcacgggc
     4321 cagcagcgcg gtagagagat ctttgtcaat aaccgcgtcc acgctttggt aatcccccgc
     4381 ttcagtacgc actaccggaa ttccaccgcc gcccgcgcca attacgacaa aaccttgttg
     4441 gatcagcgcc ttaatggcgg gtgcttcgac aatacgtttc ggttccggcg aggcgaccac
     4501 gcggcgatag ccccgtccgg catcttcaac aaaacaccag tcagggtttg ccttttgtag
     4561 tttgtcacgc tggctttcac tgaagaatgc accaatgggc ttggtgggat gggcaaaacc
     4621 tggatcgttt ttatccactt ccacctgagt taccacggtg acggcttttt tctcgccgtg
     4681 acgcgccagt cggttattca gcgcctgttg gatcagatag ccgatgccgc cctgcgtatc
     4741 tgccacacag ttcgccagcg gcgttaaggg cagcccttcg cgctcgtggg caatctccgc
     4801 acggcgtaaa tccagcccga cctgcggccc gttaccgtgg gtcagcacaa tgtcgtaatc
     4861 ggaagccagc atttccagca ccgtatcggc cacggctttc accgcctccg cctgatgctc
     4921 aatcgactgg ctggcgttat ctttgataat gctgttgcca ccaatggcaa cgaccacaag
     4981 ctctttcatg ttgttatgtc cgctacagag ggtgatgagg ttatgaccaa cgccagactg
     5041 cctgatgcgc tacgcttatc aggcctacaa ttctcgggcc atccgtaggc cggataaggc
     5101 gttcacgccg catccggcat aaacatgtaa gtcagacgcc gtaacgttcg caccagccga
     5161 gaatggcttt ctcgaagcaa gcgaatggtg gatgcacaat gcccgcgcca atcatgccga
     5221 cgcccgcatc tttatgggcg atggcggtgt tgatcaccgg cagaatgccg ctgctaccca
     5281 cgcgggtgat gtcgattgcc gacggcacgc ccataaaacc gagcagcgga atggtgacgt
     5341 tggggttttc accgagagtg atttcacgca tctgacggga gaaatcgata gcctcttcca
     5401 ccgtgccgcc caccagcgcg acgattgcag gcgcggtcgc catagcaaat ccaccaatac
     5461 cgtaggtttc ggtgatggcg ctgtcgccga tatccagccc cgaatcttcc ggcttataac
     5521 cggcaaacat cgggccgatc acctgctgcg ccgggccggt aaaccattgc cccggcaggc
     5581 cgctgacccg caggccgaac tcgacgccgt tacgcgccat ggtggtgacc acggtgctgt
     5641 attcgatgcc gtgcgccgca tccatcgccg ctttacacat cgccatccac gtcgggccgg
     5701 agaagtagtc gctgctggcg acaaactcga acacttcgcg ctgttgctcg acggaatagc
     5761 ctgcctgaat gatccccggc gtcagtgcct gaatcagcag cgtcgtcccg gcgttattgc
     5821 ggttatggca ctcatcgccc atatgcagcg cctgcgccag cattaagcgc agatcgatct
     5881 caccgataat tttcatcgcg tcgcgcagca ttggcccctg cacatcgcgc atccagttga
     5941 ggcggtcgat cacgctctga tcgttggcac ccatacgcaa aatcttcgcc atctgctcgc
     6001 tcatattggt gtaagcgatg ttgccgtagg ttttgttttt cacgatgtgc ataaacatcg
     6061 aggccgaggt aacgcccgcc atcgatccca cacagtcgtg ttcgtggcac ggcgagaagg
     6121 tgatctcccc ggaggccgcc agttcagccg cctcgtcgag atctttcgcc agtccttcga
     6181 acaccagcgc tccggtgacc gcgcctttca tcgcgccgca cattttttcc caggtgatcg
     6241 gcggcccggc gtgaagaatg gttttcggcg tcatgcccgg caccacgtta atcgcctgat
     6301 caaaaccaat cagcaccgga tgcgactgga taatgcgctc cagcgcctgc tggttagcgg
     6361 cggcgatttt gtctgccagc ggcgaatcgg caatgttatc cagcgcctgc accacctgca
     6421 tattgccctg ccccggcggc gtccagtcga gttgagtcac ttctacatgc tgctttttca
     6481 ggtcatcgct aaacatggcg atgccgacgt taataacgtt caatggttgg ctaaacagtg
     6541 actggctcat caggcttcct cccctttgca gataaattca cgcgccagca atccggtatt
     6601 ggtgctgctg ctcgccagaa tcactccggc atcaagcagc atctggcttt gttgttctaa
     6661 cgatggcgta tcaagatcgg tacccagcac ataggcgaga atgatcaact cgcgtccttc
     6721 ggcagcggcg atcgctttcg cttctttgat cgcctcgatg gtggagccga ccggatcttc
     6781 atgcgatcca aatccgagca caaaatccat cacgatcacc gccacttctg gatcgcgcgc
     6841 ctcttcgatc aagcgactga tgcggttggt ggggtcaatc attggatgcg gcttgccgtt
     6901 cgtgaagtcg tcatcgccaa agtcgaggaa ggtgtgtttg atgctgcggt tgatatcttg
     6961 caggcggaat tccggatcgg gctgaatgtt gctgtagaca tcgccatgtt tttccatcac
     7021 cgcgaacatg gtttcgtcgc acagcgtgcc gccgcagaac aggccacgaa tgtatttctg
     7081 ctgcggttgc agacgcgcac gcacatcagc aatcaacggc tggttaagcg tatgcaggtc
     7141 gagattttcc tgtttcacgc cggagagcat caccgctttt agcgccgcct ctttgctgcc
     7201 gcgggcaaac tgtagcccct gctcatccac cggcgtttca acgcgaccga ggaagcagac
     7261 caccaccggc ttgcggcagg cacgtgcgcg ttccagcact ttgcgggcca ccgcaggcgc
     7321 gggaggtttt gaaatcagtg cgataatttc agtttgcgga tcgttttcca gcatcccgat
     7381 ggcgtcgagc atcatcaggc cgccgatttt ctcgctcagg tcgcgcccgc cggtgccaat
     7441 cagttgtgaa acaccgccac caaattcatg aatgcggacg ctcagctcct ggctgccggt
     7501 gccagatgcg ccaacaatac cgatattgcc gcgacgcacg gcgttaccaa aacagagcgc
     7561 cgcgccgtta ataatcgccg tgccgcagtc cggccccatc atcagcagcc ctttttcgtg
     7621 ggccaactgc ttgagcgcca gttcatcttc aactgagacg ttatcggaaa acagcatcac
     7681 gttgagatcg ttttgcagcg cctgacgcgc ttcgcgagcg gcaaacagac cgttgaccga
     7741 aatcaccgcc aggttacttt ccgggatatg ctttttggcg ctggcaatag tggcgtaacg
     7801 cgcctcgtgc gagccgcttt gcgctttggt gttgaacagt tcttcaatct ccaccagcaa
     7861 ctgctcgttg tccgcacccg atttaccatt gatgacaatc atcaggtcgc cgtttttcgc
     7921 ctgttccagc gtcggcgtca gcagtcctaa atttttcagc acacctttgt tcatttcggt
     7981 cgccatcgcc acaaatgcct gctcgacgcc gtcgagttta tttgcacgcg tggagataga
     8041 catcagcgag acagaatcaa aataggtatt cggtttaatg actattttaa ctgacataat
     8101 tccctccgta ggtttggctc agcgcacaac catcggccat gccatacagc atgtcgcagc
     8161 cggaactgga gccaatatgt ttaatttttt ctattgcctg agttgaattc ccagggacgc
     8221 cataaataat gttgtgaata aagcgatgaa tattttcccg gcagcgttgt tgtaatgcgg
     8281 cttccagcgt tatggcactt aataacgtgg tgttatttct gccgcgtagc agaccgcgat
     8341 aaaattcctc tttgtatttt tctgccggat gcccgggaat aaataaaata agcgccagac
     8401 ctgttaaata atcatcggct gatggcgtaa ggccaatccc taacccaatc atactggcga
     8461 ccgctgcggc gatattttgc ttatcgttta ctgtctgaat aactgcccgt cgtttaatat
     8521 gtaattcttt atttaacgcc tgataaaacg gattatcgcc gcgataatta aacagggttt
     8581 cattttcccc caggtgctga tgaataatgt cgtgccactg tagccaggcg ataaggtgaa
     8641 aattcacagc ggtcatttgc aaggttgggg acagccaacg ccgacaacga ctcatctcta
     8701 tatgaagatg ttgaccaacc gtaatacctt gatcgtcaaa ctgaacctta tcaccatgcc
     8761 ggaacagatc atcaaagtga gtgagtgcca accgacaact gtttggcgca ttgtcgtact
     8821 cttcacaaag cagcgtcagc aactgctggc gagccgggag tgcgatattt accgctctgg
     8881 caaagacctg ttcaacctgt ccttgccccc gctgcgccag aaaatccgca tccgcagtta
     8941 gcgcccacac acaccgtttt tctggtgtgt tgatagccag cctcccggtt tatgcacctg
     9001 cggcaatcag taactgcgca atctcttcaa agccccgttc ccgcgccagt tccagtggcg
     9061 ttttgccata tttatcggtc agatgcgggc tggcaccgtg ttccagcaat aactgcacaa
     9121 tcgcctgctg tttaatacca ccatcattaa gcacaatcgc ttccagtagc ggcgtccagc
     9181 ctacatggtt ggtctggtta acgttaatct ccgtatgcgc caaaagctct tttacaatac
     9241 ttaaatggcc tttttcacag gcaggcgtca ggccgacacc gccaaaacgg gttacgcaat
     9301 taagatcggg tttagccggt aaaataattc gtagtagcgt taaatcatcg ttcagacagc
     9361 taattaaaaa aggatttaaa caggtatgat cttgcttatt aatatccgct ccggcatcaa
     9421 ttaatgcctg aacgcaagca tattgctgat ataaacttgc cagcgtaatt gccgttttcc
     9481 cctgacgatc gcaggtatta atatcgacac ccagcgcaag gcaggttttt actttatcga
     9541 tatcaccctg ttgtgcagcc aataaatagt cggcaggtag attttttata ctcattcaca
     9601 ttcccctggt ttttgtggct gttttcgatg aattgagaat agcaacgata atctgataaa
     9661 agaacccgct taaatttaat tcgccgtatc tcgccttttt gttgaataat attcaacaat
     9721 cttttcgcag tgtgcggtag tgcaatattt gtttctttga cccgataccc tttcactcaa
     9781 tcaggtttta tttctctata gtctgcacat gtaagagtag ctttcgtcca tgtctaacca
     9841 gaggggaaca agatgaacgg actcacggca acgggtgtca ccgtcggtat ttgtgcaggg
     9901 ctttggcagt tagtgtcttc acatgtcggg ctctctcaag gatgggaatt attaggaacc
     9961 attggcttcg tcgccttttg cagtttttat gccgcaggcg gcggtaaatc ggggtttatc
    10021 agaagtctgg cggttaatta ttccgggatg gtgtgggcgt ttttcgccgc gctgactgct
    10081 ggttggctgg cctctgtaag tggactgtcc gcgttctggg cgagcgtaat tactaccgta
    10141 cccttctcgg cggtagtggt ctggcaaggg cgtttctggt tactgtcctt tattcccggt
    10201 ggctttctcg gcatgacgct gttttttgcc agcgggatga actggacggt gacgttactg
    10261 ggttttctgg cgggtaactg cgtggggata atttcagaat atggtggaca aaaattaagc
    10321 gaagcgacga ctaaacgcga cgggtattaa aagttattca aaaatgatta tctatcttta
    10381 accgatacct ctacagtcat agctggagaa attcatagta gataatggcg agaatggttt
    10441 aatgaactca atttttaccg aagagaatct gttggcattt actaccgctg cgcgttttgg
    10501 cagcttcagt aaagccgcag aagagttggg tttaaccact tccgccatta gctacaccat
    10561 caagcgtatg gagacggggc tggatgtggt gctgttcact cgcagtaccc gcagcattga
    10621 gttaacggag tccgggcgtt attttttccg caaagctacc gacctgctga atgattttta
    10681 tgccatcaaa cgcagtatcg atactatttc gcagggcata gaagcgcgtg tgcgcatttg
    10741 tattaatcag ctgctttata cgcccaaaca caccgcacgc ttgttacagg tgctgaaaaa
    10801 gcagtttcct acctgccaga ttaccgtcac caccgaagtg tataacggcg tctgggatgc
    10861 gattattaat aatcaggcca atatcgccat cggcgcaccc gatacactac tggacggcgg
    10921 cggtattgat tacactgaaa ttggcgcgat tcgctgggct tttgctatcg ccccggatca
    10981 cccgttagcc tttgtgccgg aacccatcgc cgaaagccaa ctgcgtctct accctaatat
    11041 tatggtggag gacaccgcgc atacgattaa taaaaaagtg ggctggttgc tgcacgggca
    11101 ggagtcaatt ctggtgccag atttcaacac caaatgtcag tgccagattt taggtgaagg
    11161 aattggtttt ttgccggatt acatggtccg tgaggcgatg acgcaatccc tgctggttac
    11221 ccgacaaatc cataatccgc gccaggattc gcgcatgtta ctggcgactc agcattctgc
    11281 tacgggtcag gtgacgcagt ggattaaaaa acaatttgcg ccaaatggca tattaacggg
    11341 gatctatcag gatttactgc atcgggagaa ttaatcgctg ccaacctccc gatgcgctgt
    11401 gaatagcggt ttacatcaac cacctgcttt cattacccat tcagagataa atttattacc
    11461 cggtactggc ggagagtaca aatagccctg caaaaagtga acgcctttac cgatcattaa
    11521 atccgcctgc tcctgggttt ctaccccttc cgccacgata ctcagaccag gcttacgcgc
    11581 tagttcgaca atattgtcca caatatgacc ggagatttcg tcgacactcg ccatttgcac
    11641 aaatgactta tcgatcttaa taaaatcgac cgggaacgcc tgcaagtaac gataggtcgc
    11701 ataacccgta ccaaagtcat ccagcgcaaa ggtaatgttg tgctgatgaa ggctgtcaaa
    11761 tatcgctctg gcttctggcg ttaccggaat agggttacgt tccgttagct cgagaaccag
    11821 cttgatttta tcgttaccta atttcttaac caggttcaga cactcttttt caaatcccgc
    11881 tgccaaaaaa caacccgccg agacgttgat gccaatatgg aaattgtccg gcagcaaatg
    11941 ttttaccggc atcagaatat ccgcagtctg tttcatcagt tggcgggtca ttatgacaat
    12001 aagaccggat gactccgcca gaggaataaa ctgatccggt gggataattc ccgtttgtgg
    12061 atgttcccag cggacaagta cctcacagcc cgtcagtacc ccagtctgcg cgcagaaaac
    12121 cggttggatc cacggtttga attcgtggtt ttccaacgcc agactgatgg cttcaggcgt
    12181 gacgatatgg tgacaacggt tatcagggat ataaaaatta tttttatttc ccgtgccact
    12241 gatgacttgc ggatgatatt gaaaaaagat atcccgccag aaggtaatat cactctggat
    12301 gcctagtttt tcatacagct gcttcttttg atgtgaaatt gtctttgcac tgcgatttct
    12361 gtactgtgag atttctgtaa ccgacattcc ctccattagc agcttgagta aaagccgttc
    12421 tttttcggac acgataccag gcaacgtcaa attcaccgtt gccccgaact cttccaaaaa
    12481 aacactcaaa tcatgcgaag ccattgaaca caccttgatt aaatcttgtg cgccagctaa
    12541 atctgcgagt cttcactctg actgataaag ctggctgcaa gaaaatgaca cagagagccg
    12601 aattatctcc cgttcggctc tctctccttt tactcattat tcagatcaga attgccactt
    12661 aatgccaacc attgcagagg tgtcattata tcccctatcg gcaacctgaa cgcccacatt
    12721 cccccagaca ttcaggttgg catttagctg tccttccacc ccggttttta tctcagcaat
    12781 atttcgggct ccatcctgag tgacggacac gccatccata ctggtgctga aatccttact
    12841 gttatgcagc cagttcactt ctacaaacgg ctggaactcg cgggatttac cgtcatccat
    12901 tttatggtgg ctcttcagcc aggtttttac gccaagtcgg gtttgaacat tgccatcacc
    12961 gttgctatga acgagggttc cgttgctttc gcggtgctta tcggctttga ctcccatcca
    13021 ggtaacctgt gcttgcggct gaacatacca ttcattacgc gttccctggc tgccattaaa
    13081 ttcagctaat ttgtgtttgt atccagcttc cagtgaagcg gtaaatcctt ttgatttata
    13141 ggattcactt tgtaagtcat cccctttcac tgtgttatca aaccagctgt actgcgccca
    13201 actgtcgaga tacgcgccat tacgcgactc gtcatcggca taccaggtgg catagaggcc
    13261 ggtgctatat ccgttcacac tcgcttttgc acgataaccg gttcgcgagg aaatggtttt
    13321 gctgtcgctg ttgccatatc ccgccatgac cccaacatgc cagctgtcgc tgccgttttg
    13381 gctccactgc gcgacatcgc ctcccagttg cagaacatag cgattgcttt gggttttcag
    13441 ctggccgctg ccatcacgcc atttattatg accaccttca tggcgcatcc acatagtggt
    13501 ttgtttctgc tcacccgtca ccatgtcggt atagtacgta ttacccagac gctcatgcag
    13561 acgcgtggtg aacattgtat tcgctgctgc aaggttcgca atgtagctac ccgcctccgg
    13621 tcgcaggtca ttatccacat tcagatccgg acccggcgta ggtgtcgggt tagggttcgg
    13681 ctctggattc gggtttggct ccggattcgg catcgggtct ggctccggct gcagttcagg
    13741 agaatcactg ccgctggtca gataccagtt accactattt gccccttgtc cacgcgcgag
    13801 agtgtagtca tacgcccccg caacgatacg accagcctga acaaattcgc cctcagactt
    13861 accgtctaca tggataagtt cgataccgtt aagggttttc gcacctgtac cgccagcgtt
    13921 attcaccgta acggcagtcg tgccgctagt atcgccctcg acaaccaatt tatcggttac
    13981 tgagtcatcg ccattcagta ccgtattcat tactagttga ccgttattac cgtggtagtt
    14041 ccccttcacc actaacacat tgccagcttg ctgtcctgac attccggtca tcagcgtacc
    14101 gttgttggtc agattgccgc ccagggtgta attcacagac gcagccgctc ttttttcagc
    14161 gctattattc aacgccaggg taccgttgtt ctcaacgttt gcgctaccca atccctgcgc
    14221 atgacttacc aggacattag ccgaattagc aatgttcaca ttcgctgtca ggttgctgtt
    14281 atcgcccgcg atgtccagcg tgccgccctg aatattcagc tctccgctcc ccgttaacga
    14341 accggttgaa gtgcccccgt tggtaaccgt cagactgccg ccatttaagc tcagcagtga
    14401 atcggcgctg ccattgagcg tgcccacggt ctggctgtga ccattcatat ccagttcagt
    14461 ctctgccgcc agacgcagtt catgggtatt acccaacacg ttatcgttat tcaacaaaag
    14521 cgtcccactg cgcagatcgg taacaccggt atagtcgttg tctttgttag aaagcgatac
    14581 ggtctgaccc ttctggctgc tgaatgccag gtcaccactg ccggtaatct ttgcgctgag
    14641 atcggcggca ttctcgcttt taccgttaga gctcagcacc agagcatcgc tgtcggtagc
    14701 gtgtaaatca agctgggtca ggccgtaacc gatatacaga ccgtcattgt ttaccccact
    14761 cgtcagacga tagtcgtaag tgccgacggc cgctttttgc ccattctgct gaatatcacg
    14821 ttgggcactg ctggagagga tttgcccatt tttatcctgc aattgcagat tgcccgcatc
    14881 gcccagaact tccgcacctt gcgcatcaac cagcttaatg gcgctgttac catcatcgac
    14941 ttcagtgagc gataacgcag agtcaatatc acaagatact gagcggacaa cgtcactgtc
    15001 agaaacctga atcacaccct cgccgcgcaa atccagcgtt ttactaacgt tgaccgtccc
    15061 ttctgtcatc tgtgcacctg ctgtcagggc accaaaatca acggttccgc cggagaatcc
    15121 caaagtgcta atggagtgag cgcccttacc cactgttacc aggcttccct gacccgcagt
    15181 gatcccgcct ctctgcagaa actcagcatt cttttccagg ttaaatgtcg tgttctggaa
    15241 cgccagtgaa ccctggaacc agtctgtata agcaaactca tcgaaggaga attcgtgatt
    15301 ccccatgttt atttcaacaa tacctctacc ttgaagatcg ttatcaaata cccaatcttt
    15361 attggcattg atttgcagtt tgccatcaac atccaggtcc gcacggccga tgttcttgtc
    15421 ttcagaaata accgcgacag caccagaagc gacatcaata tatccgctga atgcatagtt
    15481 attgccggtc agcgccactg tcccactgtc gatacttatt gtaccgttgc cggaaatctt
    15541 attggcaaag gtcatgctat cgtcagcctt caggtgtaat ttcccgcggt tgttgatttc
    15601 tgcggtacct atattttggg tgatccctaa agcgccattt tgctcaattt tgactttacc
    15661 gctaaaggcg gagttatccc cggttaagta agtcgtacca ttgagcgcat tgatatagcc
    15721 caggccctcg ctgtcactga caatatccgc ggaaatagtc gcgtttttat ccgtgtggtt
    15781 taaggtgatt ttcttctcgg atgtctttgc ccacacaaac tcaatttttt cggcatcaat
    15841 aatccctgca gccttcgctg cgcttccttc ctgtgcacct aacgctaact cagagttggt
    15901 gcttaaacta atagtcggcg cagagatttt accaccttcc gaaacagcaa gtgttgtatc
    15961 ggagttttga tcataataac ccaggctaat tttctcactg gcattaactt caccgccgtt
    16021 attaactgac aggtactgat taccaccgta actgccgtaa acgcccataa agaaggtttt
    16081 agaatttacc tggctgcctt caccactgac atttccctgc aaataggtca gacggctggt
    16141 gcccttaaca tggattttgc cgccgttgga gacattaaaa tagccgcgat cgtgtctcat
    16201 gcctgagttt gactcataca aatcatcagc atataactcg ctgccagcat tggagacatt
    16261 caggaacccc gtacctttca gcaaaaagtt aagtgggcca ttgatagtta cctttcctcc
    16321 ggtaatatcc aacccaccac cagaagcgag agtcagacgc ggacgggaag tatctgtcgc
    16381 attgattacc agattggcat tatcagtcac cgtaatacgg taatcgatgc tctggttctc
    16441 tttgccaaaa tcaatagttt tatcctggtt tacgatgaca tcatcggcga ataccagaga
    16501 tgaatataaa cttatcgcgc taattaaaac agcccgggac ttttttcctt ttactctgct
    16561 ccccagctcc gagcagacgc tccaacagtt acgggacctg ttccacttta gccgatatat
    16621 tttattcatc acaatctcca gcaatacatc caaataaaac caagattgca taaacagcaa
    16681 cgcattaaat gccattctgg ttactgttca tcgcatataa aaattcaaaa attttgcata
    16741 gcattggcaa ccacatatct gagtatgtag ttcagagggt aataaaatag caagttacta
    16801 ctgaaaactt attttaagtt aacactctta actttatcat tacattcctc ccagtctcta
    16861 atacctgcgc attatatgta tccaggaact caaatcaact caaaatataa acaaaagcag
    16921 gaggaataca tttaatatac agacggtaaa agtggtaact cttattaggt atccaaatat
    16981 aagaatcgtc ttattaataa atatataact ctataggact gaatccattc ggctaataca
    17041 aaataaaatc gttaattaat aaaatataaa gtcatgaagt gatacaagag gaatttcagg
    17101 atatgacatg acaatcatat ataatatatg cataattttt tacagcgagt caatgattcc
    17161 cgcgccatat gcagacatgg cgcggtttta tgcaaaaaca atcacgcgtc cgggaacatc
    17221 acgctatggc ccggcgcttc acgatggaga tgaataaagt taaggtgccg ctcgtactgg
    17281 tcaagaatat cggtgatcac ctgctctttg ctgtagtcca tcaggtcgtt gccctggctg
    17341 ccttctaaca ggaaggtttc cagtcggtag taggtcgatt taccgctacg tgcgcggtag
    17401 gtaaagcccg gcaccgaata ttgctgcggc caaatctgat agacaaagtt ttgctcttcg
    17461 cccatatgca ccaacaaatc cagatgcccc aactgttgtc cctcttccgg cggcaggctt
    17521 tttagctcca cgtacgcgcc gcgcaaccgc agctcctgcg ccacttcttc cattgccggg
    17581 taacagaccg tctccatcat ctgtttagtg taacgcgtgc ccggataatt catcaggcgc
    17641 gatagacgtt ttttccagct caggcgatcc tgaagcccca gcggtcgcgg cgcggtatcg
    17701 cggttgacac tttcacggcg gtaatcttct accttcagag atttatacaa ccccgccatc
    17761 acgaagaaaa tcacaaagct gaacggcagc cccatgatta ccgtggtgtt ttgcagcgcg
    17821 gatatcccgt tggtcatcag catgccgagc gtcagcagac caatcgccac cgaccagaag
    17881 acgcgcagcc agccgggggc gtcgctgttg atatctttaa gctgcgaggt gaaattcccc
    17941 agcaccagcg caccggagtc cgccgaggtc acataaaaca gcaggccagt aatggtggcg
    18001 acggaggcgc taaaggtaaa cgccggatac tgcgccagca ggctgtagaa gccgcgctcc
    18061 ggatggacca tcgcttcctc ggcaaatgcc gcgccgccgt ggatgatttc atacagcgcg
    18121 ctattgccga ataccgagag ccataacagc gtgaaggtaa acggaataat caacgtgccc
    18181 agcacgaact ggcgaatggt acgcccacgc gagatacgcg ccaggaacaa gccgacaaac
    18241 ggcgaccatg ccacccacca tgcccagaag aagagcgtcc agttattcat ccactcaacc
    18301 ggacggtcga aggcaaaact gttgagcgtc atgcccataa agcgattcac atagtcgcca
    18361 acgttcagca ccagcgcatt aagcaggaac gaggtgtcgc ccataaacaa tacgaacagg
    18421 atcaatccca gcgccagcgc aacgttaagt tccgataaca cacgaatgcc cttatcgaca
    18481 ccggaggtga cagagatcgt ggcgattatc accgacaagg cgatcagtgc cgcttttgcc
    18541 gccatcgaat cgggaatatc aaacagtacg ctcaagccat agttaagctg caccacaccg
    18601 ataccgagcg tagtggcaat accgaagata gtgccgatca ccgctgcaat atccactgag
    18661 tgacctatcg gcccgttaat ccgtttaccg aagatcgggt acagcgccga gcggatggtg
    18721 agcggcaaat tataacgata gctaaagtat ccgagcgcca tgcccatcag cgcatacatc
    18781 gaccagccgg ttaagccgta gtgaaacagc gtccagacca tcgcctgacg cgcggcctca
    18841 atcgtctgtc ccgcgccttc cggcggctgc atatactgcg ttaccggttc ggctacggag
    18901 aagaacatca ggtcgatacc gatcccgcca gcaaacagca tcgccgccca actcagcagg
    18961 ctgaattccg gtttggattg ttctggcccg agcttcaccg aaccaaaacg cgaacaagcg
    19021 atacagacca caaagacaat atagagcgtt gccgccagta gatagtacca accgaaggtt
    19081 ttagaaaccc agtccagcgt gcggccagtc cacagggccg agaagtcgcg aaacaggatc
    19141 gttgtcaggg aaaaggtctg tcatccagta tccccactgt tattgttacc atttaaaatc
    19201 aaattcgtaa caattacgac acattttata ttgaacgtcc aatcaataac cgctttaata
    19261 gataaacaac gctgatgaat ggagtggcga aaatgcccaa attggggatg cagtcgatcc
    19321 ggcgcagaca actgatcgac gccacactgg aagcaataaa tgaagtgggc atgcacgatg
    19381 caacgatcgc gcagatcgcc cgccgtgcag gcgtttctac ggggatcatc agccactatt
    19441 tcagggacaa aaatggtctg ctggaagcaa ccatgcgcga tatcaccagt cagctgcgtg
    19501 acgcggtttt gaatcgatta catgcacttc cgcagggcag tgcagagcag cgattacagg
    19561 cgattgttgc cggaaacttc gatgaaactc aggtgagcag tgcggcgatg aaagcctggc
    19621 tggcgttctg ggccagcagt atgcatcagc cgatgctcta tcgtttacag caggtcagca
    19681 gccgccgctt gctgtcgaat ctggtgagcg agtttcgccg cgaattgccg cgcgaacagg
    19741 cacaggaagc gggctacggc cttgccgcgc tgattgacgg attatggctg cgcgcggcac
    19801 taagcggcaa accgctggat aaacctctcg cacactcgct gacccgtcac tttattactc
    19861 agcatttacc caccgattaa ccgaggagac gtgatgtccc gaatggcaga acagcagctt
    19921 tatatacatg gtggttatac ctccgccacc agcggtcgca ccttcgagac cattaacccg
    19981 gccaacggta acgtgctggc gaccgtgcag gccgccgggc gtgaggatgt cgatcgcgcc
    20041 gtgaagagtg cccaacaggg gcaaaaaatc tgggcggcga tgaccgctat ggagcgctcg
    20101 cgtattctgc gtcgggccgt tgatattctg cgtgaacgca atgacgaact cgcaaaactg
    20161 gaaaccctcg ataccggaaa agcatattcg gaaacctcaa ccgtcgatat cgttaccggt
    20221 gcggacgtgc tggagtacta cgccgggctg atcccggcgc tggaaggcag ccagatcccg
    20281 ttgcgtgaga cgtcatttgt ttatacccgc cgcgaaccgc tgggtgtggt ggcggggatt
    20341 ggcgcatgga actacccgat tcagattgcc ctgtggaaat ccgccccggc gctggcggca
    20401 ggcaacgcaa tgattttcaa accgagcgaa gtcaccccgc ttaccgcgtt aaagctggct
    20461 gaaatttaca gcgaagcggg cctgccggac ggcgtattta acgtgttgcc gggggtgggc
    20521 gcggagaccg ggcaatatct gaccgagcat ccgggcattg ccaaagtgtc atttaccggc
    20581 ggtgtcgcca gcggcaaaaa agtgatggct aactcggcgg cctcttccct gaaagaagtg
    20641 accatggaac tgggcggtaa atcaccgctg atcgttttcg atgatgcgga tctcgatctc
    20701 gccgccgata tcgccatgat ggcaaacttc ttcagctccg gtcaggtgtg taccaatggc
    20761 acccgcgtct tcgttccggc gaaatgcaaa gccgcatttg agcagaaaat tctggcgcgc
    20821 gttgagcgca ttcgcgcggg cgacgttttc gatccgcaaa ctaacttcgg cccgctggtc
    20881 agcttcccgc atcgcgataa cgtgctgcgc tatatcgcca aaggcaaaga ggaaggcgcg
    20941 cgcgtactgt gcggcggcga tgtactgaaa ggcgatggct tcgataacgg cgcatgggtt
    21001 gcaccgaccg tgttcaccga ttgccgcgac gaaatgacca tcgtgcgcga agagatcttc
    21061 ggtccggtga tgtcgcttct cacttatgaa tctgaggacg aagtgattcg ccgcgccaat
    21121 gataccaact acggcctggc ggcgggcatc gtgacggcgg acctgaaccg cgcgcatcgc
    21181 gtcattcatc agctggaagc gggtatttgc tggatcaaca cctggggcga atccccggca
    21241 gagatgcccg ttggcggcta caaacactcc ggcattggtc gcgagaacgg cgtgatgacg
    21301 ctccagagtt acacccaggt gaagtccatc caggttgaga tggctaaatt ccagtccata
    21361 ttctaaccag gaggtttatt tgcaatttga ctacatcatt attggtgccg gctcagccgg
    21421 caacgttctc gctacccgtc tgactgaaga tccgaatacc tccgtgctgc tgcttgaagc
    21481 gggcggcccg gactatcgct ttgacttccg cacccagatg cccgcagcgc tggcgttccc
    21541 actacagggc aaacgctaca actgggccta tgaaacagag cctgaaccgt ttatgaataa
    21601 ccgccgcatg gagtgcggac gcggtaaagg cctgggtgga tcgtcgctga tcaacggcat
    21661 gtgctacatc cgtggcaatg cgctggatct cgataactgg gcgcaagaac ccggtctgga
    21721 gaactggagt tatctcgact gcctgcccta ctaccgcaag gccgagactc gcgatgtggg
    21781 cgagaacgac taccacggtg gcgatggccc ggtgagcgtc actacctcca aacccggcgt
    21841 caatccgctg tttgaagcga tgattgaagc gggcgtgcag gcgggctacc cgcgcacgga
    21901 cgatctcaac ggttatcagc aggaaggttt tggtccgatg gatcgcaccg tcacgccgca
    21961 gggccgtcgc gccagtaccg cgcgcggtta tctcgatcag gccaaatcgc gccctaacct
    22021 gaccattcgt actcacgcta tgaccgatca catcattttt gacggcaaac gcgcggtggg
    22081 cgtcgaatgg ctggaaggcg acagcaccat cccaacccgc gcaacggcca acaaagaagt
    22141 gctgttatgt gcaggcgcga ttgcctcacc gcagatcctg caacgctccg gcgtcggcaa
    22201 cgctgaactg ctggcggagt ttgatattcc gctggtgcat gaattacccg gcgtcggcga
    22261 aaatcttcag gatcatctgg agatgtatct gcaatatgag tgcaaagaac cggtttccct
    22321 ctaccctgcc ctgcagtggt ggaaccagcc gaaaatcggt gcggagtggc tgtttggcgg
    22381 cactggcgtt ggtgccagca accactttga agcaggcgga tttattcgca gccgagagga
    22441 atttgcgtgg ccgaatattc agtaccattt cctgccagta gcgattaact ataacggctc
    22501 gaatgcggtg aaggagcacg gtttccagtg ccacgtcggc tcaatgcgct cgccaagccg
    22561 tgggcatgtg cggattaaat cccgcgaccc gcaccagcat ccggcaattc tgtttaacta
    22621 catgtcacac gagcaggact ggcaggagtt ccgcgacgca attcgcatca cccgggagat
    22681 catgcatcaa ccgacgctgg atcagtatcg tggccgcgaa atcagccccg gcacggaatg
    22741 tcagacggat gagcagctcg atgagttcgt gcgtaatcac gccgaaaccg ccttccatcc
    22801 gtgcggtacc tgcaaaatgg gttacgacga gatgtccgtg gttgacggcg aaggccgcgt
    22861 gcatgggctg gaaggcctgc gcgtggtgga tgcgtcaatt atgccgcaga ttatcaccgg
    22921 gaatttgaac gccacgacaa ttatgattgg cgagaaaata gcggatatga ttcgtggaca
    22981 ggaagcgctg ccgaggagca cggcgggata ttttgtggca aatgggatgc cggtgagagc
    23041 gaaaaaatga gtcgtgatgt gaactaacgc aggaaccgat cagattgaga gttaccgttc
    23101 cagagagggg gaccgaatcc ttatataaac actgaggtaa ctctcatgct tcatatccag
    23161 tattcatgat gctggttttt tgctgatatt taccccgaac attcctgata cagactatta
    23221 at
//
LOCUS       DAKISA010000071        23144 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.071, whole genome shotgun sequence.
ACCESSION   DAKISA010000071 DAKISA010000000
VERSION     DAKISA010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23144
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.071"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..1599)
                     /locus_tag="OVW45_002753"
     CDS             complement(<1..1599)
                     /locus_tag="OVW45_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCV5527159.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLELSLVSQQ
                     FLSLEFPQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTFCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     SGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHVQKNMNTEVLNN
                     RTTDVINNHAEKIGNNQAITVTNNQ"
     gene            complement(1809..2327)
                     /locus_tag="OVW45_002754"
     CDS             complement(1809..2327)
                     /locus_tag="OVW45_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HCV5527160.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTTPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            3024..3524
                     /gene="tssB"
                     /locus_tag="OVW45_002755"
     CDS             3024..3524
                     /gene="tssB"
                     /locus_tag="OVW45_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HCV5527161.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            3559..3783
                     /locus_tag="OVW45_002756"
     CDS             3559..3783
                     /locus_tag="OVW45_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5527162.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            3834..5309
                     /gene="tssC"
                     /locus_tag="OVW45_002757"
                     /pseudo
     CDS             3834..5309
                     /gene="tssC"
                     /locus_tag="OVW45_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
     gene            5316..5729
                     /gene="tssE"
                     /locus_tag="OVW45_002758"
     CDS             5316..5729
                     /gene="tssE"
                     /locus_tag="OVW45_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCV5527163.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            5733..7583
                     /gene="tssF"
                     /locus_tag="OVW45_002759"
     CDS             5733..7583
                     /gene="tssF"
                     /locus_tag="OVW45_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCV5527164.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQIEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWPLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            7547..8629
                     /gene="tssG"
                     /locus_tag="OVW45_002760"
     CDS             7547..8629
                     /gene="tssG"
                     /locus_tag="OVW45_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HCV5527165.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLAHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            8654..9934
                     /gene="tagH"
                     /locus_tag="OVW45_002761"
     CDS             8654..9934
                     /gene="tagH"
                     /locus_tag="OVW45_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HCV5527166.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHVAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            9931..10455
                     /gene="tssJ"
                     /locus_tag="OVW45_002762"
     CDS             9931..10455
                     /gene="tssJ"
                     /locus_tag="OVW45_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HCV5527167.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGIGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            10458..11789
                     /gene="tssK"
                     /locus_tag="OVW45_002763"
     CDS             10458..11789
                     /gene="tssK"
                     /locus_tag="OVW45_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HCV5527168.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHHARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKIFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            11794..12555
                     /gene="icmH"
                     /locus_tag="OVW45_002764"
     CDS             11794..12555
                     /gene="icmH"
                     /locus_tag="OVW45_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HCV5527169.1"
                     /translation="MDEGSLSLPPFTGHDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            12564..15341
                     /gene="tssH"
                     /locus_tag="OVW45_002765"
     CDS             12564..15341
                     /gene="tssH"
                     /locus_tag="OVW45_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HCV5527170.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTMTDASDTLLARYAKNITADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESESESEQDNTGAEPADEADREQPEETAETVSPVQRLAQLT
                     AELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLTKWLGDTIKG
                     QDLAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLT
                     TINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLN
                     LFYQAFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEVLYPVLADF
                     FKPALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVVIEPEVTDEIMSR
                     VTRAENGARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAVDGAFTADVEDAQN
                     DESVTKDETVL"
     gene            15338..16081
                     /gene="tagO"
                     /locus_tag="OVW45_002766"
     CDS             15338..16081
                     /gene="tagO"
                     /locus_tag="OVW45_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagO"
                     /protein_id="HCV5527171.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            16086..17499
                     /gene="tssA"
                     /locus_tag="OVW45_002767"
                     /pseudo
     CDS             16086..17499
                     /gene="tssA"
                     /locus_tag="OVW45_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
     gene            17608..21042
                     /gene="tssM"
                     /locus_tag="OVW45_002768"
     CDS             17608..21042
                     /gene="tssM"
                     /locus_tag="OVW45_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HCV5527172.1"
                     /translation="MLILAWIFLLVWIWWKGPMWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVRVMKRLQQLEKMQKQQREEAVDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLIRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDFGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNQ
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFITRYGLQSYFVKQRDELVELTAMDSWVLNLTRNVKYSDADRAEIQHQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KEREEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLTDQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFWAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            21053..22405
                     /locus_tag="OVW45_002769"
     CDS             21053..22405
                     /locus_tag="OVW45_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HCV5527173.1"
                     /translation="MNSNVLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQHTRKRLEPQPKSNAENAAQTTVRTLIYAPETLASSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPVESLLTQYHDARAQKQNTDALEKQINERLEGVLSRWLLLKNNVMPEAATGTTAEK
                     "
     gene            22429..22911
                     /locus_tag="OVW45_002770"
     CDS             22429..22911
                     /locus_tag="OVW45_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HCV5527174.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            22955..>23144
                     /locus_tag="OVW45_002771"
     CDS             22955..>23144
                     /locus_tag="OVW45_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5527175.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRHQNTHHVILLTLDV"
BASE COUNT         5455 a         6316 c         6299 g         5074 t
ORIGIN      
        1 ctggttattg gtaacggtga tcgcctggtt gttacctatt ttttcggcat ggttgtttat
       61 cacatccgtg gtgcggttat tcagcacctc ggtgttcatg ttcttctgca cgtggatgta
      121 gacctgttct ttccctgtcg catcgtcaaa cttcagttca ttaaacccgc tgcccttata
      181 ggttttcgaa cgaatggtca tctgcgtctt cgtccccggc aggctaccgg gtgtgcggtt
      241 ttcctggtgg taggtacgcc ccataataat cggctggtcc ggatcgccgt tgaggaagtc
      301 cacaatgacc tcctgaccca cgcgcggtat cgcaaggtta ccgaatccgg tgcctgccca
      361 cgcctgtgcc acacggatcc agcatgaact gtcctggttt gacgggttat agcggtccca
      421 gttaaatttc acccgcacgc ggccatgttc atcacagaag atttcctcgc ctgccggtcc
      481 cgtcacgacg gcgctctgcg ggccatccac cagcggtttc agcaacggct gtggccgcca
      541 tgtccggtct gccggtatta ccgcaaagtg gttattcagc gtggttcctg aaccactgcg
      601 tcccggcacc gcctgcggct gctcgccgtg cagttcactt gccaccacct gccattcccg
      661 gttcaggttc gcctgcggat gccccgtcag cacaattcgc cgtcccggcc atatctccgg
      721 agaacggctt gttccgcgcg ccacttctgc gttgttgcgc cagccatcca tctgccagcg
      781 ggcaaagttc tgcccgtgag cacccttgaa acgtccgggg tagtcataca cttcatactg
      841 tgtgcgctgg taatcctggt actggccttc ctgatcaaaa cgccctgccc agccgggtcg
      901 tttaaaggtg tagtctttgg tcaccacgga agaaggacgg atttgtgcgc tgtagcggaa
      961 ctggctgatg cagaaggtac ttacctcggt acgggtgttc gggttccagg ggatctcaaa
     1021 ggactccggc agataacgca cggtgtcgca caggaccagg ctctggtcgg tacttttttg
     1081 cgcgtgctcc tcataaaaga agatgccttc ctccgccgcc atccggcaca ggaaatcgta
     1141 atcagtttcg ccgtactgga cacaaaactc acgggaagga tgcggctcgc tgaacagcgg
     1201 actccactcg gtcacaccgt tttccttcag gatcgtcgcg aggatgcttt cgatgtcctc
     1261 attctggaag atacggaagt tctggcgcag ccctgtgcgc cacagcggtg ggcatacctt
     1321 catgctgtac agcatctggt ttttgtcgtt ctcccccagt tcaaaccagg tcaccacacc
     1381 tttcacccgg cgctgtacgt catcgccctg ccatatcgtc aggtaggcca ttttgtccag
     1441 tacctgcgga aattcaaggg agagaaactg ctggctgacc agagagagct cgagggaaaa
     1501 gagcgaagag agtgactggt tcagatgaaa ggagaccacc gcaaaagcat ccggtggcag
     1561 gccgtccact tccagcgtga aacgtaatcc ggttgacata gcaacctccg gggttaagga
     1621 aaaacagaca gttcgctgtt aaagcggctg tgacagtaca gtgttacgct tgcgttcagg
     1681 cacgctaccg ccgaagtttc cggcatccgc catctcccgt gaacgtaacc ccttactgac
     1741 aacctttctt tctacggcgt aatacaaaaa cagcatccgg cctgatagcc ggatgctgtt
     1801 ggcttaactt atgcttccag tggtgcgcgc cagtcgtcgg caccggaggt gccagatttg
     1861 acgtgttccc attcaatttt gcggtaggcc agtgaaacct cgagcaactg ggtgaactcg
     1921 cgtttcgccg ggtcctggca atgcggcata tgcagtttca tatcgacaat cgtcgaatca
     1981 gtcaggcggg tagtgaaata atgctcctgt ttgccctcca cagaggtacg ccaccagtga
     2041 agttcagtgg ttggcagcat ttcgccggag gccagtgcgt tgtacatcag cggaaccgct
     2101 ttgttcagcg ccacggtaaa aatgaaaggc ttgtgagcac gctgacccgc aggctgaccg
     2161 gactgaggag tggtcgggac agtaacgtta tgcaggaatt cctgcaccag catctcgtct
     2221 tcgtgcccct gcacatagat gttgccgaca gaatcagcgg taaatgcacc tgcagtaata
     2281 tttccctggg tctgaccagt gatggagata taacacggtg ttggcatttg taaactcctt
     2341 gttaacgtgg ttaattacta cttacaatga cctgccgtta atcccagaca ggcagatgca
     2401 gctcacttca cggaaccgca tcatttttca gtatttttta catacaaacc gagctagaat
     2461 ttacggttgt cgtaaaaact atttttaaaa tcattaaatc tcttattcag caataactta
     2521 ataatatata tctggataac catacccaac tgttcaaagt ccgtattgcc tccataaaat
     2581 ggccaaagct gaatcatgaa taaatattgt atttatcctt tctgaattat cattttgata
     2641 atttttgaga tattccttaa aaatacccac ttaatcaatc acaaaacaaa tatataaaca
     2701 agcaatcatt agattagctt atttttaatg aaattattat atacttcaat aagttacaca
     2761 aaaactctat atttaaatta ttgccataat acacctgacg cgaattgcac aggttcacac
     2821 aatctattta acgcttgcag aatacccctc tttacgatcg ataaaaagtc cttttcttat
     2881 ttattttgaa gcgtaaaatg cggggtgatg aaggagtggt gattttattt tcaccgccat
     2941 tagggcggca ttgccgatat ttttttgcgg tacaaatatg ccgtgttcat ggcctgtacg
     3001 agataacatg acggatcact tttatgagca aaaaatttga aggcagcgtc gcgccccgcg
     3061 agcgtattaa tatatcgtat gtcccaaaaa cagatggcca gacggctgaa gtggaacttc
     3121 cgcttaacat gctcgttgtc ggcgataccg gaaatacgca ggaaacatcc ccactggatg
     3181 aacgtcaggc agtttccgtt aacaagcaca atttcggtgc agtgatggct gaagcagcca
     3241 ttggcctcaa ttttactgtc cccgccacgc tgaagggaag cacaacagac gatgaactga
     3301 atgtggcgct gaatatcaaa tcactggatg atttttcacc agatagcgtg gcccggcagg
     3361 ttccggaagt gaataaattg ctggaactgc gtgaagcact gactgcactg aaggggccga
     3421 tgggtaacct tccggcattc cgtacccagc tccaggcatt gctggaaaat gaagagtccc
     3481 gcgagcagtt gctcaaagaa atcggtctgg tgagcaataa ataaccacca ctatttcatt
     3541 aaacagggtt atccaggcat gacggcacac aaaaatatct ccgggacata ccacctctct
     3601 gttgcagaca ttctgcaggt ggtttaccag gtatgtttca gcccgtccgt tgaaatcaac
     3661 caggatggtg tggctgcgct gattacaaca ctggatcgcc gtatcagtga tctattggat
     3721 gagattatcc atttttgtga gtttcagcag agcgcgtcgc actggcagcg cgtacttcac
     3781 tgatggcgaa aagccggttt cagaaacaca gctaaaacag aaggattttc agtatgtctt
     3841 tacaggaaga ggaactcgtt tccagtcatg ccgggcagcc tgagcaggca tcgtcgctgc
     3901 ttgatcaaat catggcacag acccgtattc agcccggttc ggaaggttat gacgttgccc
     3961 tccagggtgt gacagccttt attgccagca tcctgcaatc cactgcatct gccgaaccgg
     4021 tcaataaact ggccgtggac agcatgattg cagatattga tgaacgcatc agccgccaga
     4081 tggatgtcat catccatgcg cctgcctttc agcaggtcga gtcgttctgg cgctcactga
     4141 aaaccatggt ggatcgcgtt gatttccgtg aaaacatcaa ggtcaacgtg cttcatgtca
     4201 ccaagcagga acttctggaa gactttgagt ttgcgccaga aattattcag tccggatttt
     4261 ataagcacgt ttattcgtcc ggtttcggac agtttggcgg taaacccatc gctgccgtgc
     4321 tgggcgctta tgagttcaaa aataccgcgc ctgacatgaa actgctgcaa tacgtcagtg
     4381 ccgtgggcgc aatggcgcac gcaccgttcc tgtcctccgt ttccccggag tttatggggc
     4441 tgaactcgtg gaccgaactg cctaatatca aagatcttta tgccatcttt gaaggtccgg
     4501 cctacaccaa atggcgcgcc ctgcgtgact cggaagattc acgctattta gggcttacgg
     4561 caccccgctt cctgcttcgc cagccctatt caccaacaga taaccctgtt aagaacttta
     4621 actattacga agatgtcagc cagaaccacg aagattatct gtggggtaat acggcctgga
     4681 tgctggcatg caatatcgca gacagttttg ccaaataccg ctgatgtcca aacattatcg
     4741 gtccgcaaag cggcggcgca gtgaaagatc tgccgatgca tctgttcgaa acgatggggc
     4801 agattcaggc caaaatccca accgaggtgc tggtcaccga tcgccgcgaa tttgaactgg
     4861 ctgaagaggg ttttatcacc cttaccatgc gtaaagactc tgataatgca gcctttttct
     4921 ctgcaaactc ggtacaaaaa ccgaagcact ttccgggaaa agatgcagaa accaactata
     4981 agctgggtac gcagcttcct tatctcttca tcatcaatcg gttagcgcac tacatcaagg
     5041 tgttgcagcg tgaacaactg gggtcatgga aagaaaggag cgacttagaa agagagctta
     5101 atacctggat ccgtcagtat gttgccgacc aggaaaaccc gcctgcagac gtgcgcagcc
     5161 gcaaacctct gcgcgctgcc aaagttgagg ttatggatgt agaaggcgaa cccggctggt
     5221 accaggttgc gctaagcgtg agacctcatt ttaaatttat gggggcaaat tttgagcttt
     5281 ccctggttgg ccggttagac agggagtaac acaccatgat accggatcgg gaaagaacca
     5341 caggttctct ttttgagcgt atggaagcct cttcggccag aaaccggcag ggaggcagta
     5401 ttcattcgct caggcaatcc attcgccaga acctacgaaa cattcttaat acccggtccg
     5461 gtagttgccg gggtgcgcct gagctgggca ttgatgaacc ggaaggggct gaaaacttcc
     5521 gcgaatccat gagtcgggcc atcgagcaat gtatagaacg ctatgaaccc cggatctcac
     5581 acgctgaagt gcaggcagtg gtttcatccg cctccagccc gctggatatg acgtttcata
     5641 ttacagcctg ggtgacattt aacgaaacgc atgaggtgct tgaatttgat atggcaccga
     5701 atggcagcca gcattaccgg gtggactgac aaatggaatt tgaagaacgc tatttccggg
     5761 aagaactcga ttacctgcgc cagcttagca agctgctggc aacggaaaaa ccccatctgg
     5821 cccgcttcct ggccgaaaaa gatgcagatc cggatattga acgcctgctg gaaggggtgg
     5881 cttttcttac cggcaatctc cgccagaaaa ttgaggatga attcccagaa ctgacgcacg
     5941 ggcttattaa gatgctatgg cctaattacc tgcgtccggt tccggcaatg acccttattg
     6001 aatatacgcc ggatatggat aagtcttctg taccggtgtt aatcccccgt aatgagcagt
     6061 ttacaaccaa cgccggggaa atcagagttg atgaagtgct gccctctgat gctaaaaagg
     6121 aggagccgcc tccctgtacc ttcaccctct gccgggatat ctggctgctg cccgttcgcc
     6181 tggagcagat tgaaaaccgc agtacgaccc gtaatggtgt tatcaacatc accttttcgg
     6241 tcgcaccggg aacggacttc cgcacgctgg atctgaacaa acttcgcttc tggctcggca
     6301 atgacgacaa ctatacccgt gaccagcttt atttatggtt ctgcgaatac ttgcagggtg
     6361 ccgacctgac tgtgggtgaa cagcatattc gcctgcctga gtttatgcta aaagctgtcg
     6421 gttttgagcc gcaggatgcc atgctgccct ggccgaaaaa cgtccacagc ggctaccgga
     6481 tccttcagga gtatttctgt taccccgatg cgtttctctt ttttgatctt tgtggttgtc
     6541 cggctttgcc tgacggattg caggcggaat tctttaccct gcaactgcgt ttttcgcgcc
     6601 ctttgcccgt ggacatccgg ctgcgccgcg attccctgcg cctgtattgc gcacctgcca
     6661 ttaatttatt tatccaccat gcagaagcca tcacgctgga caaccggcgg gcagactatc
     6721 cgctggttcc cagccgccat tacccacaac attacgatgt attttccgtt aacagtgtgg
     6781 tgagccaggt ccaggatatg ttcaggaaaa aagatctggg gcgtcctgtt tcgacgcagg
     6841 ccgcgcgcca gtggccagcc tttgaaagtt tcagccatca gatagaatac agccggaagc
     6901 gggaagtggt gtactggcat caccggacca aaacatccct gttccatcgc ggctttgatc
     6961 atacccttgc ctttatacat gctgatggca gttatccgtc agacgaatct ctgctcagta
     7021 atgaagtggt ttcggtatcg ctgacctgta ccaaccgtga gcttccgtca caaattcgtt
     7081 ccggcgatat caccggcaca accggaaaaa atgcggctgt tgcttcattt cgcaacatta
     7141 cccgcccgac gcaaccactc tggccggtca ttgatggcag cctgcactgg cccctactct
     7201 ccgccatgaa cctgaattat ctgtcattac tggatacgga cgcgctgaag caggtcatcg
     7261 ccaactttga tcgccacgca atccatcatc cgcagacggc acggctgtca caacaaaagc
     7321 tggatgccat tgagcgtctg gagacccgcc ccgttgatcg cctgtttacg ggtattcccg
     7381 tccgggggct ggcttccacg ctgtatctgc acccggagcc gtttgtctgt gaaggggaaa
     7441 tgtatctgct cggtacggtg ctttcgcatt ttctgtcgct gtacgccagc gttaactcat
     7501 tccacatgct gaccgttgtg aacacagaaa gccaggagac atggaaatgg acggaaagaa
     7561 tcgggcagca tcctcttatc tgagtccggg aaatcccccg gcagataaag agcagaatga
     7621 tccgctggca caggttttcc ataatgcctg ctcttacaat ttttttgcga tggcggagct
     7681 gttgcaccgc ctggcaaagg gtgaaaaggg gacgccagaa ttatccctgc gcgacgatcc
     7741 ggcacaggaa accctgcgtt tcagcgccga tgccagcctt gccttcccgt gcagtgatat
     7801 cagtgcgctg aaacgggata cgtcaggggc attcaggatg accaccacct ttatggggtt
     7861 acaggggagc cagtcgcctc tccccggtta ttacctcgct cacctggcct ggaaagcggt
     7921 acatgaacaa agtccggtag gtgattttct ggatatgttc agccaccgcc tgacccagtt
     7981 tgtctggcat atctggcgta agtaccgcta tcacatcagt tttcgtaacg gcggtgtgga
     8041 tgcgttctcg cagcgcatgt actcccttgt cgggctgggc caccgccagc ttcgcgataa
     8101 gctggcgatt aatcacagca aaatgctggc gtattccggg atcctggcaa atccgggacg
     8161 atccccggaa attatctgtg gcctggtgtc ccactgcttt gatttgtcag aagtgacact
     8221 gcaaaactgg caacgtcgca aggtggatat tgaaccggat cagcaaaata gtctggggag
     8281 ctattctctt aaaaatggcg aaaaactggc gggaagatcg gtactgggca actttgttct
     8341 gggtacacga gtgcccgatc tcagcggaaa attccagctc agtattacca gcctgacccg
     8401 aaaacagttc ctctcttttc tgccgtccgg cgaaaacttc ctgccactga cgatgttcgt
     8461 gtccttcatc ctgcgcgatc agcttgcctg ggacttacat ctggggctgg cgccggagca
     8521 ggtgggcgca atgcgcctgg gcgataacaa aagtgcgctg ctgggctgga ccagcttcct
     8581 cggcacaccg gaagaacgac cctcagtcac catcagggta cggtcgtaaa ttatgataat
     8641 cacggagcag aatatgccgg aagagaaact acagacgctt tcattgcagg tcattaacgg
     8701 cagtgagctg gaaagcgggc gggcggcgcg ctgtctgttc acacagcagg gaaatgtcgg
     8761 ccatggcccc gaatgccact ggtcggtaca ggatcgtcag cagagcattc cggcccaggc
     8821 ttttaccgtt atcctgcacg atggcacatt ttgtctgcgc ccgcagacgg cacaactgtg
     8881 gctgaatcag gcaaaagtca cagcaacatc agacctaata cagttgcgcc agggcgatga
     8941 gatccagatc ggacggctga tggtgagggt tcatctgaac cggggagata ttccccatta
     9001 cgatgaggaa atggccactc ccgaaaccat cgttaccaat cgcgatatgc tcacggatac
     9061 cctgctatca acggagggtg cgccacacta tccgggaatg actcaccggc accagcttgc
     9121 agacaccgtg gtaaacggtt tttctgccga tccactccag gctcttcagt ccgaaagcct
     9181 gattaccacg ggcgatccgc tttcaggcat tgcggctgtc cggccatcgg caccgctgtc
     9241 cgatccggca agtaatgggg ggatcaatac tccgtttatg gatctgccgc ccatttatgc
     9301 cagccccggc gatcgtaatg acgacgtctc tgcggcagaa atggcgcaac gccacgttgc
     9361 ggtcaccccc ttactgcgcg gtcttggcgg ctcgcttacc atgagcaatt ccgacgatgc
     9421 ggatgatttt ctggaggagg ccggacgaac gttacaggcc gcaataaaag gtctgctcga
     9481 tttgcagcag cagcgtaaca gcctctcaga caaacatttg cgcccgctgg aagataaccc
     9541 gctgcgcctg aacatggatt acgccaccgc gctcgacgta atgtttgccg aaggtaaaag
     9601 cccggtacat ctggcggctc ccgccgccgt cagtgaaagc ctgcgcaatg tccgccacca
     9661 cgaagaagct aaccgggcag cgattgtgga gtcgcttcgt gtcctgctgg atgctttctc
     9721 accacaaaat ctgctgcgcc gctttgtgca gtaccgccgc agccatgaac tgcgccagcc
     9781 gctggatgat gccggagcat ggcaaatgta cagccattat tacgaagaac tggcctccga
     9841 tcgccagcag gggtttgaga tgctgtttaa cgaggtctac gcccaggtct atgaccgggt
     9901 gcttcgtgaa aaacagcggg agccggaagc atgaacagga aggcgtttct ggcctgcgtg
     9961 ctgatgtgcg tcatattaac cggctgcgaa acggcgaaaa aaatcagtga ggtgatcaaa
    10021 aacccggata tccaggtcgg gagcctgaag gagcaacctt cggaaataac cgtcaccctg
    10081 ctgaccgaac cggacaccaa taccaatgcg gaaggtgaat cagccgccgt ggatgtgcaa
    10141 ctggtgtacc tgactgacga ctcaaaattg ctggcagccg actatgacca gatagccagc
    10201 acgccgctac cggatgtgct gggtaaaaac tatatcgacc accaggattt caacctgttg
    10261 ccggacacca tcaaaaccct gccgccggta aagctggatg agaaaacgca gtttattggt
    10321 gtcgtggcct atttctccga tgaccaggcc actgaatgga aacaaatcga gacggtggaa
    10381 ggaatcggcc accattaccg cctgctggtg catgtacgtc aaagcagcat cgaaatgaaa
    10441 aaagaggatg aataattatg gcaaccaccc gcaacaaggt gatgtggcag gaagggatgc
    10501 tgatgcgtcc acaccatttc cagcagcagc agcgttacaa cgactacctg gataaccagc
    10561 gtttccgggc catgaatgat ttatcctggg gatttaccga actcaccctc aacaatgaac
    10621 tgctggcgca gggtaagatc atgattgaca gcgcgtcagg cacactgccc gacggcaccg
    10681 tcttttctat ccccgaccag gacgcactgc ccgatccgct gcacccgcaa aattttccgg
    10741 acgaaagaag ccgcaatatc tacctcgctc tgccggtcgc cagtgatgtg agaaatgaaa
    10801 tcagcgacgg acggcgaatc gggcgttacc ggctgaatta tgccgatgtc cgggatttgc
    10861 attcagaaga aggtgacgcg cgaacattga cgctgggtca actgacgccg cgcattatga
    10921 gcggtgcaga agatatgagc gcctatatta cgctcccgct ttgccgtatc agcgatcgcc
    10981 atgccgatgg ctccctgacg ttggatgacg attttatccc ctcctgccag aatattcagg
    11041 tcagtaagaa actgcgtgtt tatctcaaag aggtacaggg ggccattggc ggacgggcaa
    11101 gcgatctggc aaaccgtatt ggctctccgg cgcagagcgg catcgcggat gtggcggaat
    11161 ttatgatgtt gcagttactt aaccgtaacc agacccggtt tacccatcac gcccgtcgat
    11221 cccagctcca cccggaagat ttctaccttg atcttgccgg gttgctgggt gaactgatga
    11281 cctttaccga gccgtcgcgc ctgccctgcc cgcttgatgt gtatgatcat catgacctga
    11341 ccaaaatatt taaaacgctg ttaccggaag tcaaacgggc gctgcatacc gtactgtcgc
    11401 caagagccgt caatctgccg ctgcatctgc gtgacggtat ctggcaggcc gatatccatg
    11461 acacagaact gctgcaatct gccacctttg tgctggccgt ggcggcaaac atgccggtcg
    11521 atcagatcca gcgccagttt atccagcagt cgaaaatttc ctcgccggaa aaaatccgca
    11581 atatggtcag tgtgcagatc cccggtattc cattgcgtgc ccttatggtg gctccccgcc
    11641 agcttcctta ccattccggg ttcagctatt tcgaactcga caagagtggc caggcctgga
    11701 cagaaatggc tgccgccggg gccgttgcac tgcatgtttc cggcagtttc ccggatctga
    11761 acatgcaact gtgggcgata agagggtaag acgatggatg aggggtcact gtctctgcca
    11821 ccatttaccg gacacgacga aaaaagccag cgcaactacc atctcgcgct tcgtggcaac
    11881 agcctgaatc cgatgatcga cgcggccacg ccgctactcg gtatggtaat gcgcctttca
    11941 accatgaaca gccagacgat gccggagcat ctttttgctc aggtggtgac agatgttcag
    12001 gccgtggagc aactgcttca ggagcagggc tacgaaccgg gcgtgattat ctcgtttcgc
    12061 tacatcctct gcacgtttat tgatgaagcg gcgctcggta acggctggtc aaacaagaac
    12121 gagtggatca agcagtcatt gctggtccat ttccacaacg aagcctgggg tggcgagaaa
    12181 gttttcatcc tgctggagcg cctgatccgt gaacccaaac gttaccagga tctgctggaa
    12241 tttctctgga tctgtttttc tctgggattt cgcgggcgct acaaagtggc agcacaggat
    12301 cagggggaat ttgagcagat ctaccgccgt ctgtaccacg ttctgcataa actgcgcggc
    12361 gatgcccctt tcccgctgtt acaccaggac aaaaaaaccc agggcgggcg ttatcagctc
    12421 atcagtcgcc tgactgttaa acatattttt tgtggtggcg ttgtcgtgct ggcgttgttt
    12481 tacctgttct atctgttgcg gctggacagc cagacgcagg acatcctgca ccagctaaac
    12541 aaattacttc gataaggacg tttatgatcc agattgatct tcccacgctg gtaaaacggc
    12601 tgaacctgtt ctcccgccag gcgctggaga tggccgcctc tgaatgtatg agtcagcagg
    12661 cagcggaaat taccgtcagc catgtgctca ttcagatgct cgccatgcca cgcagtgacc
    12721 tgcgggttat tacccgccag ggcgatattg gcatggaaga gttgcgccag gcgctgacgg
    12781 tagagaacta cacaaccgcc cgttctgcgg acagctaccc ggcgttttcc ccgatgctgg
    12841 ttgagtggct taaagagggc tggctgctgg cgtcggctga gatgcagcac agcgaactgc
    12901 gcggcggcgt gttgctgctg gccctgctgc attcgccgct gcgttatata ccgcctgctg
    12961 ccgcccggct gttgaccggc attaaccgtg accgtctgca acaggacttt gtgcagtgga
    13021 cacaggagtc ggcggaatca gtcgtgccgg atgcagacgg taaaggcgca ggcacaatga
    13081 cggacgcctc tgacaccctg cttgcccgct atgccaaaaa cataaccgca gacgcccgta
    13141 acggcaggct tgacccggta ctgtgccgcg accacgaaat cgacctgatg atcgacattc
    13201 tctgccgccg ccgtaaaaac aacccggtgg tggtgggcga agcgggcgtg ggcaaaagcg
    13261 cactgattga agggctggcg ctgcgcatcg tggcaggcca ggtgccggac aagctgaaaa
    13321 acaccgatat catgaccctt gacttgggcg cattgcaggc cggggcgtcg gtgaagggtg
    13381 aattcgaaaa acgtttcaaa gggctgatgg cggaggtcat ttcctccccg gtgccggtca
    13441 ttctgtttat cgacgaagca cataccctga ttggcgcggg caaccagcag ggcgggctgg
    13501 atatctccaa cctgctcaaa ccggcgctgg cgcgcggcga gctgaaaacc atcgccgcca
    13561 ccacctggag cgagtacaaa aaatacttcg aaaaagatgc cgccctgtcg cgccgcttcc
    13621 agttggtgaa ggtcagcgaa cccaacgctg ccgaagccac cattattctg cgcggtctgt
    13681 cggcggtcta tgaacagtct cacggcgtgc tgattgatga tgacgccttg caggccgctg
    13741 cgacattaag cgagcgttat ctctccgggc gtcagttacc ggacaaagcg attgatgtgc
    13801 tggataccgc ctgcgcccgt gtggccatca acctgtcgtc gccgccgaag caaatctcgg
    13861 cgctgaccac tctgagccac cagcaggagg cggaaattcg ccagcttgag cgcgagcttc
    13921 gcatcggact gcgtaccgac acatcacgga tgaccgaggt gctggtgcag tatgatgaaa
    13981 cgctgacggc gctggatgaa ctggaagcgg cctggcacca gcagcagacg ctggtccggg
    14041 agattatcgc gctgcgccag cagttactgg gcgtggcaga ggacgatgcg gcgccgttgc
    14101 cggacgcaga taccgtggag gatacgcagc cagagtcaga gtcagagtca gagtcagagc
    14161 aggataatac cggtgccgaa ccggctgatg aggccgacag agaacaaccg gaagagaccg
    14221 ctgaaacagt ttccccggta cagcggctgg cccagctcac tgccgaactg gacgccctgc
    14281 ataacgaccg gttgctggtc tccccgcacg tcgataaaaa acagattgcg gcggtgattg
    14341 ccgaatggac cggcgtgccg ctcaaccgcc tgtcgcagaa tgaaatgtcg gtcatcaccg
    14401 acctgacgaa atggctgggt gacaccatca aaggccagga cctggcgatt gccagcctgc
    14461 ataagcatct actgaccgca cgcgccgacc tgcgtcgtcc gggacgcccg ctcggtgcgt
    14521 tcctgctggc tggccccagc ggtgtgggta aaaccgaaac cgtcctgcaa ctggcagagc
    14581 tgctctacgg cggtcgccag tacctgacca ccatcaatat gtccgaattc caggagaaac
    14641 acaccgtctc gcggctgatt ggttcgcctc cgggctacgt tggctacggt gaaggcggcg
    14701 tactgaccga agcgattcgc cagaaaccct actcggtagt actgctcgat gaagtggaaa
    14761 aagcgcaccc ggatgtgctc aacctgttct accaggcgtt cgataagggc gaaatggcag
    14821 acggtgaagg ccgcctgatt gactgcaaaa atatcgtctt cttcctgacg tccaacctcg
    14881 gctaccaggt aatagtcgag catgccgatg acccggaaac catgcaggaa gtactgtatc
    14941 cggtgctggc cgacttcttc aaacctgccc tgctggcgcg tatggaagtg gtgccgtatc
    15001 tgccgctgtc gaaagagacg ctcgccacca ttatcgccgg aaaactggcc cgtctggata
    15061 acgtgctgcg cagtcgcttt ggtgcagaag tggtcattga accggaagtg acggacgaaa
    15121 tcatgagccg cgtcacccgc gcggaaaacg gcgcgaggat gctggaatcg gtcatcgatg
    15181 gcgacatgct accgccgctc tcgctgctgc tgttgcagaa aatggcggct aacacggcga
    15241 ttgcccggat tcggttgtcg gcagtggacg gcgcatttac ggcagacgtg gaagatgctc
    15301 agaacgacga gtccgtcaca aaggatgaaa cggttttatg agcaggttgc gcgttttccg
    15361 gcagacagga tggtttatcg cgggcctgat gacgggctta cccgccactg ccgcgcccgc
    15421 agaagcatcg tcaatggccg gtgtggcggt cgccgtcgcc accacgaccc cgccggatgc
    15481 gacagccacg ttacaggcca tgcagtcctg tcggcgggag tccgcggcgc tggagcggct
    15541 tgactgctat gaccgcctgc tggcaccgct gtccccgtca gggtttgacg gggcgctggt
    15601 caaagccggt tttgtgggcg aggcctggac ccgcgccaca gagcaggaaa aacgccgcga
    15661 gggcaacacc acagaactgc tggtgacgca ggtgccgggg gagcgcccga cggtggtgat
    15721 aaccaccccg gctatcggtc atgttccgcc acgcccggtg ctgatgttca gttgcgtgga
    15781 caatatcacc cggatgcagg tggcgctgat gcaccctctg gatgtccatg atatcgccgt
    15841 cactctgaat gccgacaacc gggcattgcg cagccactgg tttgtgcgcg agaacggcac
    15901 cctgctggag tccagccggg ggttatccgg gattgatgaa attaagcaac tgttcggcgc
    15961 aaaaacgctg acggtggata ccggcgcaga caatgccgcc ggaaagctga cctttaacat
    16021 tgacgggctg gcaagggcga ttgctcccct gcgcgatgcc tgccactggg cgggagaata
    16081 aaaatatggc aatggactta cgcgatccga atgtctggat atcgcacctg ctggaaaacc
    16141 tgccggaaga gaaactggca tcggcgctga aagatgacaa cccgaactgg gagtatatcg
    16201 acggcgaaat cgtcaagctg ggatcgctgg cccacagtca actggatatc ccggaactcc
    16261 agcgccgggg gctggtcatt ctggcttcgg agagtaagga ttttcgtctg ctggcgcatc
    16321 tgctaagaac cctgcaacac gccggtgatc ctcatctcgc attgcgcctg ctggcgctgt
    16381 atgtggagca ttactgggcg gtggctgcgc cgcagaatat ggcgcataaa aaacgctttg
    16441 ccagccaggt cattaaacgc tttgaaacag gtattgaagt tttttcacag aacgccgcca
    16501 cagcgcagcg cgatgcgctg tcaggggaac tggcaaaact ggcagcaatg ctggcagtca
    16561 cataacgccc cggaactggc acaggctact gatgatcttt ttgccctgta ccagcgtgcg
    16621 tttaatcgtg cggcaccggc tcctgtcccc actccggcgg cctccggtag ttcaccacag
    16681 accaccgcca cgtctgaaag cggcgtgacg caacccagtg ctccggctcc ccaaatcgtc
    16741 atcgacagtc acgacgacaa agcctggcgc gacacgctgt taaaagtggc ggccatttta
    16801 tgtgaacgcc agccggattc accgcagggc taccgtctgc gccgccatgc gctttggcag
    16861 aacatcacca gtacgcccca ggcggaaagc gatggccgta ctccactggc tgcggtctcc
    16921 gccgatatgg tggcagatta ccatgctcag ctcggtagtg ccgatatggc gctgtggcag
    16981 caggtggaga aaagcgtact gctggctcct tactggctgg atggtcactg cctttctgca
    17041 cagacggcgc tgcgtctggg ttacaaacag gtggcagacg ccatccgcga tgaggtcatc
    17101 cgcttccttg agcgtctgcc ccagcttacc gggctgctgt ttaatgacca cacaccgttt
    17161 atcagtgagc agacgaaaca atggctggca gcttcgcccg acgccaaagt tgcaccggtt
    17221 gcgcaaatcg gtgaggaatc gaaggcagcc agagcctgtt ttgctgagca gggtctggag
    17281 gcggcgctgc gatatctgga catgctaccc gaaggcgatc cccgcgatca gtttcaccgc
    17341 cagtaccttg ccgcacagtt gacggaagag gcggggctgg tacagcttgc gcagcagcag
    17401 taccggatgt tgttcaggat gggattgcag atgatggtgg cagactggga gccgtccctg
    17461 cttgagcagc tagaacaaaa attcacggca gaacaataaa agagaacagg agtttttgtg
    17521 ttcagattac caacaccccg actactcagc ggactcaaat cagccctgcg accggcgatg
    17581 ccccggttta aagtctctgc tttctggctg ctgatactgg cgtggatttt tctgctggtg
    17641 tggatctggt ggaaaggccc gatgtggacg ctgtatgaag aacagtggct caaaccactg
    17701 gcgaaccgct ggctggcaac ggcggcgtgg gggattattg ccctggtgtg gcttaccgtc
    17761 cgggtgatga agcgcctgca acagcttgaa aaaatgcaaa agcaacagcg cgaggaagcc
    17821 gttgatccgc tcagcgtgga actgaacgcc cagcagcgtt atcttgaccg ctggctgctg
    17881 cgcctgcaac gccatctcga caaccgccgt ttcctgtggc agttgccgtg gtatatggtc
    17941 atcggcccgg cgggcagtgg caaaacgaca ctgctgcgcg aggggtttcc gtccgacatt
    18001 atttatgccc cggagggcgc acgcggcgca gaacaacgcc tgtacctcac gccccatgtc
    18061 ggtaaacagg cggtgatctt tgatatcgac ggcacactct gcgctcccgc tgatgcggat
    18121 atcctgcatc gccgtctgtg ggaacatgcc ctcggctggc tgaaagaaaa gcgcgcgcgc
    18181 cagccgctga acgggattat tctgacactc gatttacccg atctgctgac cgcagacaaa
    18241 cgccgccgcg agcatctgtt acagacgctg cgcagccgct tacaggatat acgccagcat
    18301 cttcactgcc agttaccggt ttacgtggta ctgacccggc ttgatttatt acagggtttt
    18361 gccgccctgt tccagtcact caacagacag gatcgcgatg cgattctggg cgtcacgttc
    18421 acccgccgtg cccatgaaaa tgatgactgg cgaacagagt tgaatgcttt ctggcagaca
    18481 tgggtggatc gaatgaatct ggcgttgccg gatctgatgg tcgctcagac tcacacccgc
    18541 gcgtctttat tcagtttttc ccgccagatg cagggaagcc gtgaaccgct ggtgtcactg
    18601 cttgagggtc tgcttgatgg cgaaaatatg aacgtgatgc tgcgtggtgt ctatctcacc
    18661 tcttcgcttc agcgtggaca gatggatgat atattcaccc agtctgccgc ccgccagtac
    18721 cggctgggca ataacccact ggcgtcctgg cccctggtgg acaccgcgcc ttatttcacc
    18781 cgcagcctgt tcccgcaggc attactcgca gagcctaatc tggcaacaga gagccgcgcc
    18841 tggctgatac gttcccgtcg ccgcctgacg gttttctctg ccacaggcgg cgtggcagca
    18901 ctgctgctca tcaccggctg gcatcactat tacaacggta actatcagtc cggcatcacc
    18961 gtgcttaagc aggccaaagc ctttatggac gtgccgcctc cgcaggggga agatgacttt
    19021 ggcaacctgc aactgccgct cctgaacccg gtacgcgatg ccacactggc ctatggcgac
    19081 tggggcgacc gcagccgtct ggccgatatg ggactgtacc agggacgacg tatcgggcct
    19141 tatgtggaac agacctatct gcaactgctg gagcaacgtt acctgccctc gctgtttaac
    19201 gggctggtca aagcgatgaa cgccgcgccg ccggagagtg aagaaaaact cgcggtgctg
    19261 cgcgtgatgc gaatgctgga ggacaaaagc ggacgtaaca atcaggtggt gaagcagtat
    19321 atggcaaaac gctggagcga aaagttccac ggtcagcgcg atatccaggc acaactgatg
    19381 tcccatcttg actacgcgct ggctcatact gactggcacg cagagcgtca ggcgggcgac
    19441 ggtgacgcca tcagccgctg gacgccatat gacaagcccg tggtatcagc acagaaagaa
    19501 ctgagcaaac tgcctgtcta ccagcgggtt taccagagcc tgaaaacgcg ggcgctgggc
    19561 gttcttcctg ccgacctcaa tctgcgtgac caggtagggc caacctttga ccaggtgttt
    19621 acatctgccg atgacaacaa actggttgtt ccacagttta tcacccgcta cggcctgcaa
    19681 agctattttg taaaacagcg cgatgaactg gttgaactga cagcgatgga ttcctgggta
    19741 cttaacctca cccgcaatgt gaaatacagt gacgccgacc gtgcggaaat ccagcaccag
    19801 ttgaccgagc agtatatcag cgactacacc gccacctggc gggctgggat ggacaatctg
    19861 aatatccgca attttgagtc catcggacaa ctgaccgggg cgctggagca ggttatcagc
    19921 ggcgaccagc ctttgcagcg ggcgctgacc gtgctgcgtg acaacacaca gccaggcgtc
    19981 ttttctgaaa aactctctgc caaagaacgg gaggaagccc tggcagagcc ggattaccag
    20041 ttactcaccc gcctcgggca tgaattcgcc ccggaaaaca gtaccctggc agtacagaaa
    20101 gacaaagaaa gcacgatgca ggccgtgtat cagcaactca ccgagttgca ccgctacctg
    20161 ctggcaatcc agaacgcgcc tgtaccaggg aaatcggcgc tgaaagccgt gcagttacgg
    20221 cttgatcaga acagcagcga tccgatattc gccacccgcc agatggcaaa aacgctgcct
    20281 gctccgctca accgctgggt tggcagactg actgaccagg cctggcatgt ggtgatggtg
    20341 gaggctgttc attatatgga agtggactgg cgcgacagcg tggtgaaacc gtttaacgag
    20401 caactggcaa ataactatcc gtttaatccg cgttctgcac aggatgcctc actggatgcc
    20461 ttcgaacgct tctttaaacc ggatggcata ctggatacct tctaccagca gaacctgaag
    20521 ctgtttatcg ataatgacct gagtctggag gatggcgata acaacgtcat tattcgcgaa
    20581 gatattattg cgcaactgga aactgcgcag aaaatccgtg acatcttctt cagcaaacag
    20641 aacggtctgg gaacatcctt tgccgtggaa acggtatcgc tttcaggcaa taaacgccgc
    20701 agtgtactga accttgacgg tcagttagtc gattacagcc agggccgtaa ctataccgcc
    20761 catctggtct ggcctaacaa catgcgcgaa ggcaacgaaa gtaagctgac gctcatcggc
    20821 accagcggca acgcgccgcg cagtatcagc ttcagcgggc cgtgggcgca gttccgcctg
    20881 ttctgggccg gacaactgac cggagtacag gatggcaact ttaccgtgcg ctttagcgtg
    20941 gacggtggcg cgatgaccta ccgtgtgcat accgacacgg aagataaccc gttcagcggt
    21001 gggttgttca gccagtttgg tctgtcagac acactgtact gaggaggcag ggatgaatag
    21061 taacgtactg acacaaacta tcgttaccgg cagtgacccg cgcgggctgc cggaattcag
    21121 cgccatccgc gaggaaataa acaaagccag ccacccgtca cagcctgagc tgaactggaa
    21181 actggtggag tcgctggcgc tggcgatttt taaagccaac ggtgtggatt tacacaccgc
    21241 cacctactat acgcttgccc ggacacggac acagggactg gcgggattct gcgaaggtgc
    21301 ggaactgctg gcggcaatgg taagccacga ctgggataag ttctggccgc agggcggccc
    21361 ggcgcgtact gaaatgctgg actggtttaa ctcccgaacc ggcaatattc tgcgtcagca
    21421 aatctccttt gcggaatccg acctaccact gatctaccgc acagagcggg cattgcagct
    21481 tatctgcgat aagctccagc aggtggaact gaagcgcgtt ccgcgcgtgg agaatctgct
    21541 ctattttatg cagcacacgc gtaaacggct tgaaccccag ccgaagagta acgctgagaa
    21601 cgccgcacag accacggtca gaacgctgat ttatgccccg gaaacactgg catcttccac
    21661 accagaagcg gtagtgcctc ccctgcccgg cctgcctgag atgaaagtgg aagtgcgcag
    21721 tctgacagag aatcccccac aggccagtgt gataaagcaa ggcagtacgg taagagggtt
    21781 tatcgcaggg atcgcctgtt cagtggctgt cgcctcagca ttgtggtggt ggcaggtcta
    21841 tccggtgcag cagcaactgt tacaggttaa cgacaccgct cagggcgcag caacggtgtg
    21901 gatggcctca cctgaactcg aaaactatga gcgcaggctg caacaacttc ttgatacctc
    21961 cccggtacag ccgctggaaa ccgggatgca gatgatgcgt gttgccgaca gtcgctggcc
    22021 ggaaagcctg caacagcaac aggcctcgac acaatggaat gaggcactca aaacccgcgc
    22081 acagagtagc ccgcagttgc gtggctggtt gcagacccgc caggacttac atgcttttgc
    22141 agatctagtg atgcagcgcg agaaagaggg actaaccctt tcctatatca aaaatgtcat
    22201 ctggcaggcg gagcggggac tggggcagga aacccccgtt gagtctctgt tgacgcagta
    22261 ccatgatgcc cgtgcgcaga agcagaatac agatgcgctg gaaaaacaaa ttaatgagcg
    22321 actcgaaggc gtgttaagcc gctggctgct gctgaagaat aacgtcatgc cagaggcggc
    22381 aaccggtacc acggccgaaa aataattatt taattaaagg agattatcat ggcaaatata
    22441 agttatttat ctttgagtgg agagacacaa gggctaattt cagcaggttg ctcaacgctt
    22501 gattctgtcg gaaacaaagc gcagccagaa cataaagatc agatcatggt ttatgcgtta
    22561 atgcattcca tctcaagaag tcaaaatgtc aatcatcatg aattaattat caccaaaccg
    22621 gttgataaat cctcaccatt gctggcaaaa gcacttagtg acaatgaaaa aatggcaata
    22681 tgtgaattta tactctaccg aaccagtaaa gcgggtattt accagcctta ttataaaata
    22741 aatctgtcaa aagcccgaat atcaagtatt gattttgtca caccgcatgc tgtcctggaa
    22801 aaagaactgg aaccacagga gagaattgcc tttatttatg aagatatttc ctgggagcat
    22861 acgttagccg gtacgaatgc aatgagtaaa tggcaggatc gtgtacagta acttactgta
    22921 ataacataat aaattactat tgcggagaga taaaatggct ggtttaagga ataacaacaa
    22981 cacacagaat gcacagtggg ctgattatgt gggtgacatt cttcgggggg ctcagccgat
    23041 aaaccagcta gtacctcagc acccttatct taacgacgtc ccactcattg atgagttaag
    23101 gcatcagaac actcatcatg ttattctctt gacacttgat gttg
//
LOCUS       DAKISA010000072        22325 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.072, whole genome shotgun sequence.
ACCESSION   DAKISA010000072 DAKISA010000000
VERSION     DAKISA010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22325
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.072"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            486..701
                     /gene="traY"
                     /locus_tag="OVW45_002772"
     CDS             486..701
                     /gene="traY"
                     /locus_tag="OVW45_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HCV5527176.1"
                     /translation="MSRGRTRAAPGNKVLLILDETTNQKLLAARDRSGRTKTNEVFIR
                     LKDHLNRFPDFYNSSIVKEEAEDIDDI"
     gene            744..1109
                     /gene="traA"
                     /locus_tag="OVW45_002773"
     CDS             744..1109
                     /gene="traA"
                     /locus_tag="OVW45_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HCV5527177.1"
                     /translation="MDAILSVQGASAPVKKKSFFSKFTRLNMLRLVRAVIPVAVLMML
                     FPELAMASAGKGTDLMAKGQETVKATFGKDSSIVTWVILAEVLVGAVMYMMTKNVKFL
                     AGFAILSVFIAVGMAVVGL"
     gene            1124..1435
                     /gene="traL"
                     /locus_tag="OVW45_002774"
     CDS             1124..1435
                     /gene="traL"
                     /locus_tag="OVW45_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HCV5527178.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            1457..2023
                     /gene="traE"
                     /locus_tag="OVW45_002775"
     CDS             1457..2023
                     /gene="traE"
                     /locus_tag="OVW45_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HCV5527179.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNVIQGVNNYRLQNE
                     QRTAVTPMAFNAPFAVSQNSADASYLQQMALSFIALRLNVSSETVDASHQALLQYIRP
                     GAQNQMKVILAEEAKRIKNDNVNSAFFQTSVRVWPQYGRVEIRGVLKTWIGDSKPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            2034..2738
                     /gene="traK"
                     /locus_tag="OVW45_002776"
     CDS             2034..2738
                     /gene="traK"
                     /locus_tag="OVW45_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003982266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HCV5527180.1"
                     /translation="MFGGLFFSCSVMAGAGTLAPTVVPMVNGGQASIAISNTSPNLFT
                     VPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLSIQAVPREG
                     AGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQVPVTKETLQ
                     APAGLSSVADAVWTGNHLKMVRFAVENKTLSALNIRESDFWQPGTRAVMFSQPAGQLL
                     AGARMDVYVIRDGEGN"
     gene            2738..4165
                     /locus_tag="OVW45_002777"
     CDS             2738..4165
                     /locus_tag="OVW45_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraB"
                     /protein_id="HCV5527181.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGAASAIGGVLYLSDVDMSGNGET
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRSDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQTPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPGRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQNRAEEDNPESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            4155..4745
                     /locus_tag="OVW45_002778"
     CDS             4155..4745
                     /locus_tag="OVW45_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HCV5527182.1"
                     /translation="MANNMSSRQACHAARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAA
                     DAADYVRETDRSLMQLVTALWATLALMYVSLAAITGKYPVRPGKMKCIRVVTADEHLK
                     EVYTEDASLPGKIRKCPVYFPDDRTNRNNGDKNEHA"
     gene            4732..4956
                     /locus_tag="OVW45_002779"
     CDS             4732..4956
                     /locus_tag="OVW45_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HCV5527183.1"
                     /translation="MNMRNINIIKSSPESLPHNFNIAAVGVMKKGMSMSLFSSPGHIV
                     VAGQTCAGKANLTAELISAVVGNNKKGEGQ"
     gene            4953..5468
                     /gene="traV"
                     /locus_tag="OVW45_002780"
     CDS             4953..5468
                     /gene="traV"
                     /locus_tag="OVW45_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HCV5527184.1"
                     /translation="MKKISLFVPLLGTLLLSGCAGTSTEFECNATTSDTCMTMEQANE
                     KAKKLEQPADAKPAAASLPRLADGNFRTTSVQAVAATTPSRSRPAVTALPEQKLLAPR
                     PLFTAAREVKTIVPVSSVAPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            5603..5863
                     /gene="traR"
                     /locus_tag="OVW45_002781"
     CDS             5603..5863
                     /gene="traR"
                     /locus_tag="OVW45_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HCV5527185.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQKINAHGIPVYLCEACGNPV
                     PEARQKIFPGVALCVECQAYQENKIREKQIQALNDAHSVKER"
     gene            5989..8619
                     /gene="traC"
                     /locus_tag="OVW45_002782"
     CDS             5989..8619
                     /gene="traC"
                     /locus_tag="OVW45_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HCV5527186.1"
                     /translation="MNNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRL
                     LPYRDYNQESGLFMNDTTMGFMLEAIPINGTNESIVEALDHMLRTKLPRGVPFCIHLM
                     SSQLVGDRIEYGLREFSWSGEQAVRFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYR
                     VFFSYCSPSKKKSRADILEMENLVKIIRASLQGASIATQTVDAQAFIDIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPELSISCPFILTLTLMVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETVLEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAESPTIRSRLDEMI
                     VLLDQYTANGTYGRYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRNLKKLNVIDEGWRLLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDS
                     DKASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFQPLQRDMIGKFGAARDQWFS
                     SFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWKKSG
                     PEMASLEAWLEEHEKYRGVA"
     gene            8616..9002
                     /gene="trbI"
                     /locus_tag="OVW45_002783"
     CDS             8616..9002
                     /gene="trbI"
                     /locus_tag="OVW45_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HCV5527187.1"
                     /translation="MTTRQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PVTVAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            8999..9631
                     /gene="traW"
                     /locus_tag="OVW45_002784"
     CDS             8999..9631
                     /gene="traW"
                     /locus_tag="OVW45_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HCV5527188.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            9628..10620
                     /locus_tag="OVW45_002785"
     CDS             9628..10620
                     /locus_tag="OVW45_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HCV5527189.1"
                     /translation="MKRSLWLLMLFLFAGHVPAVSADSACEGRFVNPITDICWSCIFP
                     LSLGSIKVSQGKVPDTANPSMPVQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFCHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVTRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            10650..10955
                     /locus_tag="OVW45_002786"
     CDS             10650..10955
                     /locus_tag="OVW45_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5527190.1"
                     /translation="MATMDSKYIQHLRKISTVLLMLISRTLKLFPFGLVMVPVILFYF
                     APDSFLAEIIHEWKMADISEQVTLVRLYLKVIFTCAFVAVFLNYLFSDEFMPVNSRK"
     gene            10964..11602
                     /gene="trbC"
                     /locus_tag="OVW45_002787"
     CDS             10964..11602
                     /gene="trbC"
                     /locus_tag="OVW45_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HCV5527191.1"
                     /translation="MNRKIFALFALTAGMHLAVHASENVNTPENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPWQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVAHDLLAGKGDSGK"
     gene            11599..13407
                     /gene="traN"
                     /locus_tag="OVW45_002788"
     CDS             11599..13407
                     /gene="traN"
                     /locus_tag="OVW45_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HCV5527192.1"
                     /translation="MKRILPLILALVAGMAQADSNSDYRAGSDFAHQIKGQGSSSIQG
                     FKPQESIPGYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKD
                     ILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEISRSEYTNYTCERDLQVEQYCTRT
                     ARMELQGSTTWETRTLEYEMSQLPAREVNGQYVVSITSPVTGEIVDAHYSWSRTYLQK
                     SVPMTITVLGTPLSWNAKYSADASFTPVQKTLTAGVAFTSSHPVRVGNTKFRRHTAMK
                     LRLVVRVKKASYTPYVVWSESCPFSKELGKLTKTECTEAGGNRTLVKDGQSYSMYQSC
                     WAYRDTYVTQSADKGTCQTYTDNPACTLVSHQCAFYSEEGACLHEYATYSCESKTSGK
                     VMVCGGDVFCLDGECDKAQSGKSNDFAEAVSQLAALAAAGKDVAALNGVDVRAFTGQA
                     KFCKKAAAGYSNCCKDSGWGQDIGLAKCSSDEKALAKAKSDKLTVSVGEFCSKKVPGV
                     CLEKKRSYCQFDSKLAQIVQQQGRNGQLRISFGSAKHPDCRGITVDELQKIQFDRLDF
                     TNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            13434..13691
                     /gene="trbE"
                     /locus_tag="OVW45_002789"
     CDS             13434..13691
                     /gene="trbE"
                     /locus_tag="OVW45_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HCV5527193.1"
                     /translation="MKVIFTSNRIVDFIIRLLITAIVISPVIIWSWDTVKETTADGML
                     AAAFVILYSGVLLFILYFCFSALTDLQKTDERKSDERNEDE"
     gene            13684..14427
                     /gene="traF"
                     /locus_tag="OVW45_002790"
     CDS             13684..14427
                     /gene="traF"
                     /locus_tag="OVW45_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HCV5527194.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINLKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSARKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            14441..14782
                     /gene="trbA"
                     /locus_tag="OVW45_002791"
     CDS             14441..14782
                     /gene="trbA"
                     /locus_tag="OVW45_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HCV5527195.1"
                     /translation="MHTGDLCFFTGASLFIVGVVGYFFTGPNNKGVYLLSSFVFFVVN
                     VISVCMLPDNFLFLCGAVMNQCAVPVVSMAVSTMPVITKFDSFRRMFVCIMLSAIWSG
                     VMWFFIISLVK"
     gene            15180..15464
                     /gene="traQ"
                     /locus_tag="OVW45_002792"
     CDS             15180..15464
                     /gene="traQ"
                     /locus_tag="OVW45_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HCV5527196.1"
                     /translation="MISKHRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSREEREALEARQQAMMEGRQEGGHVSH"
     gene            15451..15996
                     /gene="trbB"
                     /locus_tag="OVW45_002793"
     CDS             15451..15996
                     /gene="trbB"
                     /locus_tag="OVW45_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HCV5527197.1"
                     /translation="MSLTKLLPVILLMMATGVQASTRDEIERLWNPQGMAAKPAQPAA
                     GVPARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPETLPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     AGFMARMDTVLQMYGGKKGAK"
     gene            15926..16300
                     /gene="trbJ"
                     /locus_tag="OVW45_002794"
     CDS             15926..16300
                     /gene="trbJ"
                     /locus_tag="OVW45_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HCV5527198.1"
                     /translation="MPPVLWRGWIPFCRCTEEKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIRQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWILVSEIYYSRMTGLNESS
                     GISEETYDDRKQKESGIQNQKN"
     gene            16221..16631
                     /gene="trbF"
                     /locus_tag="OVW45_002795"
     CDS             16221..16631
                     /gene="trbF"
                     /locus_tag="OVW45_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HCV5527199.1"
                     /translation="MNHQEYQRKLMMTENKKNQEFKIRKIKRDIERGCDNAEKYFWLF
                     VVFFVAGLLLWHGMHAIFSAGIDSWKANPELNNLIYMWNILMYAIPYTLYALAAGFLV
                     TFFSVPNVRINIREYHDIPAETPYAPGEQIKGGQ"
     gene            16634..18007
                     /locus_tag="OVW45_002796"
     CDS             16634..18007
                     /locus_tag="OVW45_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HCV5527200.1"
                     /translation="MLRIKPLFILCAALLMVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNVQLISMTLPDINAGCGGIDAYLGSFSFINGEQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDYLQKLASDINSMNLSSCQVAQGLVGG
                     LFPQTQESQRKVCQDIGSESNIFADWAASRQGCTVGGQSSSVRDKASDKDKERVLKNQ
                     NIIWEALKKNKMLDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDILRAIMEGG
                     TAKIYHCNDSDKCLKVVADANVTIARDKALKSQITKLLTSIQNKAVSDTPLDDREKGF
                     ISSTTIPVFKYLIDPQMLGVSNSVVYQLTDYIGYDIMLQYIQELLQQARAMIATGNYP
                     QAVMDNVLENLNQAQQQIAVFQSQVQVQQDALLVVDRQMSYMRQQLSARMLSRYQNNY
                     HFGGGTL"
     gene            18004..20850
                     /gene="traG"
                     /locus_tag="OVW45_002797"
     CDS             18004..20850
                     /gene="traG"
                     /locus_tag="OVW45_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating pair stabilization
                     protein TraG"
                     /protein_id="HCV5527201.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVQVHRVDNVPVGLAMPL
                     SLTTRIGHAMVVSYEMIFTQPDSVTYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASSDPYTIIFSQPSPLRQVPNNNYSFLHSSEPFVSCK
                     DASVGLKDKLNFDTNTGGKTWHYYVQQIFGGRPDPDLLFRELLSDSYSYFYGASQSAS
                     QIMRKNVTINALKEGITSNAARNGDTASLVSLATTSSMEKQRLAHVSIGHVTMRNLPM
                     VQTILTGIAIGIFPLLILAAVFNKLTLSVLKGYVFALMWLQTWPLLYAILNSAMTFYA
                     KQNGAPVVLSELSQIQLKYSDLASTAGYLSAMIPPLSWMMVKGLGAGFSSVYSHFASS
                     SISPTDSAAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMTYQTGSGATTTQTRD
                     GNMVMDASGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNTLS
                     QFGSNRGSSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATRELASRSA
                     TASAGLYGDAHAGLDAKPSLFGVRVDVGAKVGGRASIDGSDLDSHEASSGSRASHDAR
                     HDIDAKASKDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTN
                     MTCSHEYAEMASRTESMSGQMSEDLSQQFAQYVMKRAPQDAEAILTNTSSPEIAEHRR
                     AMAWTFVQEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIEQRTQ
                     DSNIRNDVKHQVDNMVTEYRGNIGDTQSSIRGEENIVRGQYSELQNHHKTEALSQNNK
                     YNEERESQKIIPEPSEEAVKKMVDDKKDRLKGPL"
     gene            20847..21356
                     /gene="traS"
                     /locus_tag="OVW45_002798"
     CDS             20847..21356
                     /gene="traS"
                     /locus_tag="OVW45_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898910.1"
                     /note="TraS, as the term is used in F-like plasmids, is a
                     plasmid entry exclusion protein. It interacts with the
                     F-like plasmid mating stabilization protein TraG; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer entry exclusion protein TraS"
                     /protein_id="HCV5527202.1"
                     /translation="MITHYDIKTETQKLKDVLSVEGVNIPPLLQVIKPGGYVFLWVLL
                     WPTFLRLLADKVDIRDAGFDICFSGVMGFILFVAITNGMMLYLAIPKKFRDESKVISF
                     MYDKNKTYILLFLIAFSMVSFAHTLLYEFLLIALFIIFFFIYAIDINRYNLSAIVSVI
                     GLFKKESVS"
     gene            21370..22155
                     /locus_tag="OVW45_002799"
     CDS             21370..22155
                     /locus_tag="OVW45_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5527203.1"
                     /translation="MKTKKLMMVTLVSSTLALSGCSSVGNIKHVEPSKYVHVTDYLND
                     AFEKANCVGSIFLSQGRIFTDADGLKYQLNWPNTKCSSNSIEYAVEKYCIHKGGKMVQ
                     EGRWCKVNGEPLFYVDYGSNWKHYGENFMAVEQGSFQGRQQWLNAAERLGFLSKRVQL
                     QQKLEDEINAKKEAAELQRKMKEKNTPVNADVGDLICKEDYEAKPYQYPGTAYYKAYV
                     EKKEKNKLQLRLVWHGGNGFVINDITPSNNIIWSSPNGWKHCN"
BASE COUNT         5728 a         5127 c         6027 g         5443 t
ORIGIN      
        1 tatggagtgt gatcatagaa agtgttatca caccatgcgg ctatttcttc atctggaggt
       61 ttatttgggt agcaaatgat aatctgtcat catttgttgt gtctaaatac tccaatgaat
      121 taattgtacc ctctgatgaa tatgtaggca ttgagccata tctgataggg tttagccatc
      181 attattcatc agcaaagatg aacatcactg tgtctaaatc aaagaagaaa accatgaaat
      241 tatttaaacg atatggattt agttctagag atctcttgtt ggatgaaatg attagaacgg
      301 aaaagatact tcctttatat gctaaagtaa aagaaatatt gggaagataa gctgttaact
      361 tattgttact ttgcgggaac cattggttcc cgtttttgtt aatataattt actctatttt
      421 tgttttcatt aaagatgatt tatatgatta tgttttatta tctcaagtaa ttatgcggag
      481 ggaacgtgag cagagggaga accagagccg caccaggtaa caaagtctta ttaatactgg
      541 atgagacaac taatcagaaa cttttagcag ccagggatcg tagcggaaga acaaaaacta
      601 atgaggtgtt cattagacta aaagatcatc tcaatcgttt tcccgacttt tataattcat
      661 ccatagtaaa ggaggaagct gaagacatag acgatatata aagagtttta ttttttacta
      721 tcaatattaa acagaggaat catatggacg ccattttaag tgttcagggt gcttctgcgc
      781 ccgtcaaaaa gaagtcgttt ttttctaaat tcactcgtct gaatatgctt cgcctggttc
      841 gtgcagtgat cccggttgct gttttgatga tgttgttccc tgagttagct atggcttctg
      901 ctggtaaagg cacggacctg atggctaaag gtcaggaaac ggttaaagca actttcggta
      961 aggactccag tattgttaca tgggttatcc tggctgaagt gctggtcggt gcagtcatgt
     1021 acatgatgac caaaaacgtc aagttcctgg ccggttttgc catactctct gtatttattg
     1081 ctgtgggtat ggccgtcgtt ggcctctgac aggaaataaa acgatgtcgg gagacgagaa
     1141 taaacttaag aaatatcgtt tcccggaaac actgaccaac cagagccgct ggtttggcct
     1201 gccactggat gaactgatcc ccgcagcaat ctgtattggc tggggtatca caacatcgaa
     1261 atatctgttc ggtattggtg cagcggttct ggtttatttc gggattaaaa aactgaaaaa
     1321 agggcggggc agttcctggt tacgtgacct gatttactgg tatatgccaa cagccctgct
     1381 gcgcggtatt tttcataatg ttcccgattc gtgtttccgg cagtggatta aatagaactg
     1441 atatcaggat tgttatatgg aacacggtgc tcgtttaagt accagtcgtg taatggccat
     1501 cgcctttata tttatgtcag tgcttattgt tctcagcctc tctgttaacg tcattcaggg
     1561 ggtgaataac taccgtcttc agaatgagca acgcactgcc gtgacgccaa tggcatttaa
     1621 tgcccccttt gccgtgtcgc agaacagtgc cgatgcctct tatttacagc agatggcgct
     1681 gtcatttatt gccctccgtc tgaatgtttc atcagaaacc gtcgatgcct cacatcaggc
     1741 gcttctgcaa tatatccgcc cgggcgcaca gaaccagatg aaagttattc tggctgaaga
     1801 agcgaagcgt attaaaaacg ataacgtgaa ctcagccttt ttccagacca gtgttcgtgt
     1861 ctggcctcag tatggccgtg tggaaattcg tggtgtgctt aaaacctgga taggtgattc
     1921 aaaacctttc actgatatca aacattacat ccttattctg aagcgggaaa acggggtgac
     1981 ctggctggat aatttcgggg aaacagacga tgagaaaaaa taatacggca gtaatattcg
     2041 gcggcctgtt tttttcctgc agcgtgatgg ccggagccgg tacgctggcc cccaccgtgg
     2101 tgccaatggt gaacggtggt caggccagta ttgccatcag caataccagc ccgaatctgt
     2161 ttaccgttcc cggtgaccgg attatcgccg tgaacagtct ggatggtgcc ctgaccaata
     2221 atgagcagac cgcctccggc ggtgtggtgg ttgccaccgt caacaaaaag ccctttacgt
     2281 tcattctgga aacagaacgt ggtctgaatc tttccattca ggccgttccc cgtgaaggcg
     2341 cggggcgtac cattcagctg gtcagtgacc tgcgcggaac cggagaagaa gccggcgcgt
     2401 gggaaacgtc cacgccttac gaatccctgc ttgtaaccat cagccaggcc gtccgtggcg
     2461 gaaaattacc cgcaggctgg tatcaggtcc cggtgacaaa ggaaaccctg caggccccgg
     2521 cggggctgtc ttcagtggca gatgccgtat ggacggggaa tcacctgaag atggtccgct
     2581 ttgccgtgga aaataaaacg ctgtctgccc tgaatatccg ggaaagtgac ttctggcagc
     2641 cgggaacccg tgccgtgatg ttcagccagc ctgccggcca gttactggca ggtgcgcgca
     2701 tggatgtgta tgtcatccgt gacggggagg gcaactgatg gccagtatca acaccattgt
     2761 gaaacgcaag cagtacctgt ggctggggat tgtggttgtc ggtgcagcct ccgcgattgg
     2821 tggtgtgctg tatctgtctg atgtggacat gtccggtaac ggtgaaaccg tggctgaaca
     2881 ggagcctgtg ccggatatga ccggtgtggt ggatacgacc tttgatgaca aggtgcgtca
     2941 gcatgccacc acagagatgc aggtgacggc agcgcagatg cagaagcagt atgaggaaat
     3001 ccgtcgtgaa ctggatgtcc tgaacaaaca gcgcagtgat gaccagcgtc gtattgaaaa
     3061 gctgggacag gacaatgcag ccctggcaga gcaggtgaaa gccctgggtg caaatcctgt
     3121 caccgcgacc ggtgagccag taccgcagac gccagcctca ccgcccggcc cggaaggcga
     3181 accacagcca ggaaacaccc ccgtatcctt cccgccgcag ggcagcgttg ctgttccacc
     3241 gccgacggcg ttttatcccg ggaatggtgt cacgccacca ccacaggtga cgtaccagtc
     3301 tgtgccggtg cctggccgga tacagcgtaa ggtgtttacc cgtaatgagg gaaaacaggg
     3361 accatcgctg ccgtacattc cgtcaggaag ttttgcgaaa gccatgctga ttgaaggagc
     3421 ggatgccaat gcctcagtca ccggtaatga atccacggtg ccgatgcagc tgcgtatcac
     3481 cggcctggtg gaaatgccga acagcaagac gtatgacgca acgggatgtt ttgtgggtct
     3541 ggaagcctgg ggagatgtct ccagtgaacg tgccattgta cgcacccgca atatcagctg
     3601 cctgaaggac ggcaaaacta ttgatatgcc gattaagggg catgtcagct tccggggcaa
     3661 aaacggtatc aagggcgaag tggtgatgcg taacggtaaa atcctcggct gggcatgggg
     3721 cgcgggattt gttgacggta tcggtcaggg aatggagcgt gcctcccagc cggctgtcgg
     3781 gctgggggcc actgccgctt acggggccgg tgatgtcctg aaaatgggta ttggtggcgg
     3841 cgcatcgaaa gccgcacaga cgctcagtga ctactacatc aaacgtgctg aacagtatca
     3901 cccggtgata ccgattggtg cgggcaacga agtgaccgtg gtgttccagg acggcttcca
     3961 gctgaaaacc gtggaagaga tggcgctgga acgcacgcag aacagagcgg aagaagacaa
     4021 tccggaaagt ccggttcctg ttccgccatc ggctgaaagt catcttaacg gctttaatac
     4081 tgaccagatg ctgaagcagc tgggcaacct gaatccgcag cagtttatgt ccggcagcca
     4141 gggagggggc aacgatggca aataatatgt cgtcccgtca ggcgtgccat gccgcccggt
     4201 atgttgtcgc ccgggttctg cgaggactgt tctggtgtct gaagtacacc gtcattctgc
     4261 cactggccac aatggccctg atggctctgt ttgttttatg gaaggacaac accacgccgg
     4321 ggaaattact ggttaaggag attaacttcg taagacaaac agcacctgcc ggacagttcc
     4381 cggtcagtga atgctggttt tcttcctctg attcttcagg cagatcagaa atacaggata
     4441 tctgccatta ccgcgcggct gatgctgcag attatgtcag ggagactgac cggtcactga
     4501 tgcaactggt caccgcatta tgggcaacac tggcactgat gtatgtctcg ctggcagcga
     4561 tcaccgggaa atatccggtg cgacccggaa aaatgaaatg tatccgggtg gtaaccgcag
     4621 atgaacatct gaaggaagtt tatacggaag acgcttccct gccgggaaaa atcaggaaat
     4681 gccctgttta ttttcctgat gacagaacaa acagaaacaa cggagataaa aatgaacatg
     4741 cgtaatatta atattattaa aagcagtcct gaatcactgc cgcacaattt taatatagcc
     4801 gctgtcggtg tgatgaagaa gggtatgtcg atgagtttat tttcttctcc ggggcatatt
     4861 gtggttgccg gacaaacctg tgcggggaaa gcaaatctga cagcagagtt aatttctgct
     4921 gttgtgggga ataataaaaa aggagaaggg caatgaaaaa gatttcttta tttgttcctc
     4981 tgctgggaac cttattactt tccggctgtg ccggaaccag tacggaattt gagtgtaacg
     5041 ccaccacatc cgatacctgt atgacgatgg agcaggccaa tgagaaggcc aaaaagctgg
     5101 aacagcctgc agatgcaaag ccggctgcgg catcactgcc gcgcctggct gacgggaact
     5161 tccggaccac gtcggtgcag gccgttgcgg cgacaacgcc gtcccgcagc aggcctgcgg
     5221 tgacggcact tcctgagcag aaactgctgg caccacgccc gctgtttacc gctgcccggg
     5281 aagtgaaaac gattgttccg gtcagttcag ttgcgccggt aacgcctccc cgtccgctaa
     5341 gaacgggtga gcagacggca gcattatgga tagcgcctta tattgataat caggatgttt
     5401 atcatcagcc atccagtgtg ttttttgtta ttaaaccgtc tgcgtgggga aaaccacgta
     5461 ttaattaact ggccctgaag ttgtatatca gccggcaatg ccggcgggct ttctgtattc
     5521 aaaatctgcc tgtaaaacga gtgccctgtg cgtgaaaagg gatgggcggt agcgtgtcag
     5581 aacatgtcgg gagtaatatt ccgtgagtga tgaagccgat gaagcatatt cagtgacaga
     5641 acaactgacc atgacaggaa taaaccggat acgccagaaa ataaatgctc atggtattcc
     5701 tgtttatctc tgtgaagcat gcggaaatcc tgttccggaa gcccggcaga aaatatttcc
     5761 cggtgtggcg ttatgtgttg aatgtcaggc gtatcaggaa aataagattc gcgaaaaaca
     5821 aattcaggca ttaaatgatg ctcattcagt aaaggaacgt taatcctggt ttcctgttaa
     5881 aagcctccat ctcttatttg tgctgtggtt tcaggaatgg cactgcgtga tatatccacg
     5941 ggcacagact gatattttcc ttttttaaac gtactaagga atacccatat gaataataac
     6001 ccacttgagg ccgtcactca ggcggttaac tccctcgtca ccgcactgaa actgcctgac
     6061 gaatccgcaa aggccaatga agttctgggc gaaatgagct tcccgcagtt cagccgtctg
     6121 ctgccgtacc gtgattacaa ccaggaatcc ggtctgttca tgaatgacac cacgatgggc
     6181 tttatgctgg aagccattcc catcaatggg acgaatgagt ccattgtgga ggctctcgat
     6241 cacatgctgc gcaccaaact gccgcgcgga gtgccgttct gcatccatct gatgtccagt
     6301 cagcttgttg gtgacagaat tgaatacgga ctgcgtgagt tctcctggtc tggcgaacag
     6361 gcagtacggt ttaatgccat tacccgggcc tattatatga aagcggcagc gacacagttt
     6421 ccgctgccgg aggggatgaa tctgcccctg accctgcggc attaccgggt atttttctcg
     6481 tactgttctc cctcaaagaa aaaaagccgg gctgacattc tggaaatgga aaacctggtg
     6541 aaaatcatcc gggcgtcgtt acagggtgcc agtatcgcca cacagacggt ggatgcacag
     6601 gcctttatcg atattgtcgg ggagatgatt aaccataacc cggactccct gtacccgaaa
     6661 agacgtcagc tggacccgta ttctgatctg aattatcagt gtgtggagga cagttttgac
     6721 ctgaaggtcc gggcagatta cctgacgctg ggtctgcgtg agaacggcag gaacagcacg
     6781 gcccgtatcc tgaatttcca tctggcccgt aacccggaaa tcgccttcct gtggaacatg
     6841 gccgataact acagcaacct gctgaacccg gaactgtcca tctcctgtcc gttcatcctg
     6901 acgctgaccc tgatggtgga agaccaggtg aaaacccaca gcgaagccaa cctgaagtac
     6961 atggacctgg agaaaaagtc gaagacctcc tatgccaaat ggtttccgtc cgtggagaaa
     7021 gaagcgaagg agtgggggga actgcgtcag cggctgggct ccggtcagtc ctccgtggtg
     7081 tcctacttcc tcaacatcac agcattctgc aaggacaata atgaaacggt actggaagtg
     7141 gagcaggaca tcctgaacag cttccgtaaa aacggttttg agctgatttc accgcgcttt
     7201 aaccacatgc gcaatttcct gacctgcctg ccctttatgg ccgggaaagg actgtttaaa
     7261 cagctgaaag aggccggtgt ggttcagcgc gcagagagct ttaatgttgc caacctgatg
     7321 ccgttagtgg cggataaccc cctgacaccg gcaggtctgc tggcacctac ctaccgtaac
     7381 cagctggcgt ttatcgatat tttcttccgg gggatgaata acaccaacta caacatggcg
     7441 gtctgtggca cctccggggc cggtaaaacc gggctgatac agccacttat ccgcagcgtg
     7501 ctggactccg ggggctttgc cgtggtgttc gacatggggg atggctacaa gtccctgtgt
     7561 gagaacatgg gcggggtgta tctggacggt gaaaccctgc gttttaaccc gtttgcgaac
     7621 atcaccgata ttgaccagtc agcggagcgt gtccgtgacc agttgtcggt gatggccagc
     7681 cccaacggta acctggatga agtgcatgaa ggtctgctgc tgcaggcggt cagggcctct
     7741 tggctggcca aagagaacag agcacgtatt gatgacgtgg tggatttcct gaaaaacgcc
     7801 agtgacagcg agcagtatgc cgagtcaccg accatccgca gccgtctgga tgaaatgatt
     7861 gtgctgcttg accagtacac tgccaacggc acttacggtc ggtattttaa ctccgatgag
     7921 ccgtccctgc gggatgatgc gaaaatggtg gtgctggagc tgggcggact ggaagaccgt
     7981 ccgtcactgc tggttgcggt gatgttcagc ctcattattt acatcgagaa caggatgtac
     8041 cgcacgccgc gtaacctcaa aaaactgaac gtcattgatg aaggctggcg tctgctggac
     8101 ttcaaaaatc acaaggtcgg tgaatttatt gagaaaggtt accgtaccgc ccgccgtcat
     8161 accggtgcct atatcaccat cacacagaac atcgtcgact ttgactctga caaggcatcc
     8221 agtgctgccc gtgcggcatg gggtaactcc tcctacaaaa ttatcctcaa acagagtgcg
     8281 aaggagttcg cgaaatacaa ccagctgtat ccggaccagt tccagccact gcagcgcgac
     8341 atgattggta agtttggtgc ggccagagac cagtggttca gttccttcct gttgcaggtg
     8401 gaaaaccatt cctcctggca ccgcctgttt gtggacccgt taagccgcgc catgtacagc
     8461 tctgacggcc cggattttga gtttgtgcag cagaaacgca aagaggggct gagtattcat
     8521 gaggcagtgt ggcagctggc gtggaagaag tcagggccgg agatggcttc gctggaagcc
     8581 tggctggaag aacatgaaaa atacaggggt gttgcatgac cacaaggcaa aaaacgactg
     8641 atgttacagc cccccgacgc agccactggt ggtggacggt gccgggatgc ctggcaatgg
     8701 tgttactgaa cgcagccgtc agttacggca ttgtcagact gaacgcaccg gtgactgtcg
     8761 ccttcaacat gaaacagacc gtggatgcgt tttttgacag cgccagtcag aaacaactgt
     8821 cagaagcaca gtcaaaagcc ctttcagcgc gttttaacac ggcgctggaa gccagcctgc
     8881 aggcgtggca gcagaaacac cacgcggtca ttctggtgtc gcctgctgtg gtacaggggg
     8941 caccggatat cacccgggaa atccagcagg atattgcccg gcgaatgagg gcggagccat
     9001 gagatgccgg gggctgattg ccctgctgat atgggggcag agtgtggccg ccgccgatct
     9061 tggtacctgg ggcgatctgt ggccggtaaa ggagccggac atgctgacgg tgatcatgca
     9121 gcgcctgacg gcactggagc agtccggtga gatgggccgg aaaatggatg cctttaagga
     9181 gcgggtgatc cgcaacagcc tgcggccccc tgccgtgccc ggtatcggac gcacggagaa
     9241 atacggcagc cggttgtttg acccgtccgt cagactggct gcggatatcc gggataacga
     9301 agggcgggtg tttgcccgtc agggtgaagt gatgaatccg ctgcagtatg ttccttttaa
     9361 ccagacgctg tatttcatca atggcgatga tccggcgcag gtggcctgga tgaaacgcca
     9421 gacgccgccc acactggaga gcaaaattat cctcgtgcag ggcagtatcc cggagatgca
     9481 gaagtctctg gacagccgtg tttactttga ccagaacggt gtgctctgcc agcgcctcgg
     9541 cattgatcag gtcccggcac gggtgagcgc cgttcccggc gatcgcttcc tgaaggtgga
     9601 atttattccg gcagaggagg gcagaaaatg aagcgaagcc tgtggctgct gatgttattc
     9661 cttttcgccg gtcatgtccc tgcggtgtct gcggattctg cctgtgaggg gcgttttgta
     9721 aacccgatca cggatatctg ctggagctgt attttcccgc tctcgctggg cagtatcaaa
     9781 gtcagtcagg gtaaggtccc tgacacggcg aacccgtcga tgcccgttca gatttgtccg
     9841 gcaccgccgc cgctgttcag gcgtatcgga ctggccattg gttactggga gccgatggcg
     9901 ctgacggacg tcacccggtc accgggatgc atggtgaacc tgggcttcag cctgccggct
     9961 tttggtaaaa cggcacaggg aacggcgaaa aaggatgaga agcaggtgaa cggggcgttc
    10021 tgtcacgttc actggtacaa atacccgctg acgtactggc ttaacatcat cacatcgctg
    10081 ggctgtctgg aaggtggtga cctggatatc gcttatcttt ctgaaattga ccccacctgg
    10141 acggacagca gcctgaccac cattctcaat ccggaagctg tcatctttgc caatccgata
    10201 gcacaggggg cctgtgcagc agatgccatt gccagcgcct ttaatatgcc tctcgatgtt
    10261 ttgttctggt gtgccggttc gcagggcagt atgtacccgt tcaatggctg ggtgagtaat
    10321 gagtccagtc cgttgcagtc ctccctgctg gtcagtgaac gcatggcgtt caagctgcac
    10381 cgtcagggca tgattatgga aaccatcggg aaaaataacg ccgtctgtaa tgaatatccg
    10441 tccccaatcc tgcccaaaga acgctggcgt taccagatgg tgaatatgta tccggacagc
    10501 ggacagtgcc acccgttcgg gcgcagcgtg acccgctggg aaaccgggaa aaatccgccc
    10561 aacacaaaga aaaacttcgg ctacctgatg tggcgtaaac gtaactgtgt attcctgtaa
    10621 atcattttaa tgaataagag gaaatgaaaa tggcaacaat ggacagtaaa tatattcagc
    10681 acctcaggaa aataagtact gtgctgctca tgctgataag cagaacatta aaattatttc
    10741 cttttggtct tgtaatggtg cccgttatat tattttattt tgcacctgac agttttctcg
    10801 ctgaaattat tcatgaatgg aagatggcag atatatcaga acaagtgaca cttgtcagat
    10861 tatatctgaa ggtgatattt acatgtgcgt ttgtggctgt ttttctcaat tatctttttt
    10921 ctgatgagtt catgcctgta aatagcagaa agtgagaaat atcatgaacc ggaaaatatt
    10981 tgcacttttt gctctgacag cagggatgca tcttgctgtt catgcttcag aaaatgtgaa
    11041 cacacctgaa aaccgccagt tcctgaagca gcaggaaaat ttaagcagac aactgcgtga
    11101 aaaacctgac catcagctga aagcctgggc ggagaaacag gtgctggaaa acccccttca
    11161 gcgttcagat aaccattttc tggatgagct ggttcgtaaa cagcaggctt cacaggacgg
    11221 gaaaccctgg cagggagccc tgtattttgt gtcgttttcc attcccgaag aggggctgaa
    11281 acggatgctg ggcgaaaccc ggcactacgg tatacccgcc acactgcggg gcatggtgaa
    11341 caatgacctg aagaccaccg ctgaagctgt gttgtccctg gtgaaagacg gcgcgactga
    11401 tggtgttcag atcgacccga cactgttttc acagtacggc attcgcagtg taccggcact
    11461 ggtggtgttc tgcagtcagg gatacgacat catccgggga aacctgcggg tcggccaggc
    11521 gctggagaaa gtggccgcca cgggtgactg ccggcaggtg gctcatgatt tactggcagg
    11581 gaaaggagat tccgggaaat gaaacgtatt ttacctctga tactggctct ggttgccggc
    11641 atggcacagg ctgacagcaa cagtgattac cgggccggtt ctgattttgc tcaccagatc
    11701 aaagggcagg gaagcagcag tattcagggc tttaagccac aggagagtat ccccggctat
    11761 aacgcgaatc cggacgagac aaaatattac ggtggcgtga ctgccggcgg ggatggcggc
    11821 ctgaaaaatg acggcaccac ggaatgggcg accggtgaaa ccggaaaaac catcacagag
    11881 tcattcatga acaagccgaa agacattctt tcaccggatg caccgtttat ccagaccggc
    11941 agggatgtgg tgaaccgggc tgacagcatt gtgggaaaca ccgggcagca gtgcagtgcg
    12001 caggagatta gccggagtga atacacgaat tacacctgtg agcgggattt acaggtggag
    12061 cagtactgca cacgaactgc ccgtatggag ctgcagggga gtaccacatg ggaaacccgg
    12121 acgctggagt atgaaatgag tcagctacct gcacgtgaag tgaatggtca gtatgttgtc
    12181 tccattacct ctcccgttac cggtgaaatt gtcgatgcgc attacagctg gagtcgtact
    12241 tacctgcaga agagtgtacc tatgacaatt acagtactgg gaaccccact gagctggaat
    12301 gccaaatact cagcagatgc ctcatttaca ccagtacaga aaacactgac tgccggtgtt
    12361 gcttttacgt catctcaccc cgtccgggtc gggaatacaa aattcagacg tcatacggca
    12421 atgaagctgc gtctggttgt cagggtaaaa aaagcctcgt acaccccgta tgttgtctgg
    12481 tctgaaagct gcccgttcag caaggagctg gggaaactga caaaaacaga atgtacggag
    12541 gctggcggga acaggacgct ggtgaaagat ggtcagtcat acagcatgta ccagagctgc
    12601 tgggcatacc gggacacgta tgtcacgcag tcagcagaca agggaacctg tcagacctac
    12661 accgacaatc ctgcctgtac cctggtgtca caccagtgcg ccttttactc cgaagaaggt
    12721 gcctgtctgc atgaatatgc cacgtactcc tgcgagtcaa aaacatccgg gaaagtgatg
    12781 gtctgtgggg gagacgtctt ctgtcttgat ggcgaatgcg ataaggctca gagcgggaag
    12841 agtaatgatt ttgccgaagc tgtttctcag ctggcagcac tggccgctgc aggtaaggat
    12901 gttgcggcgc tgaacggggt ggatgtccgt gctttcaccg gtcaggcgaa attttgtaag
    12961 aaggctgcag ccggatacag caactgctgt aaggacagcg gctggggaca ggatatcggg
    13021 ctggccaaat gcagcagtga tgaaaaggcc ctggctaaag caaaatcaga caaactgacc
    13081 gtcagtgtcg gagagttctg ttcgaagaaa gtgccgggtg tctgtctgga gaaaaaacgc
    13141 agttactgtc agtttgattc gaagctggcg cagatagtcc agcagcaggg gcgcaacggt
    13201 cagttgcgta tcagttttgg cagtgcgaag catcctgact gtcgggggat tacggttgat
    13261 gagctgcaga aaattcagtt cgacagactg gatttcacta acttctacga agacctgatg
    13321 aataaccaga aaatcccgga cagtggcgtt ctgacgcaga aagtgaaaga gcagattgct
    13381 gaccagctga aacaggcagg acagtaaggc agggaaaata accggggaaa atgatgaagg
    13441 tcatattcac ttctaatcgt attgttgatt ttattatccg gcttttgatt acagcgattg
    13501 tcatttcccc ggtaattatc tggtcgtggg atacggtaaa agaaaccacg gcagacggga
    13561 tgcttgccgc tgctttcgtt atcctttact ccggtgtgct cctgtttatt ctgtattttt
    13621 gtttttctgc cctgactgac ctgcagaaaa ctgatgaaag aaaaagtgat gaaaggaatg
    13681 aagatgaata aagcattact gccactgtta ctctgctgct ttatttttcc ggcgtcaggg
    13741 aaagatgcag gctggcagtg gtataacgag aaaataaatc tgaaggaaaa agaaaataaa
    13801 cctgtacctg cagccccccg tcaggaaccg gatattatgc agaaactggc cgcactgcag
    13861 acggcaacaa agcgggcgct gtacgaagcc attctgtatc ccggcgtgga taattttgtg
    13921 aaatatttcc ggctgcagaa ttactggact cagcaggccg ggcttttcac catgagcgcc
    13981 agaaaggcca tgctggcaca tcctgaactg gactataacc tgcagtacag ccattacaac
    14041 ggcacggtac ggaaccagct ggcggcagat caggcgcagc agcgacaggc cattgcgaaa
    14101 ctggctgaac actacggcat catgtttttt taccgggggc aggaccccat cgacgggcaa
    14161 ctggcgcagg tcattaatgg cttccgggat acgtatggtc tgagtgttat tcccgtttcc
    14221 gtggatggcg tgattaatcc gctgttgccg gattcccgga ctgaccaggg gcaggcgcag
    14281 cgcctcggcg tgaaatattt cccggccatg atgctggttg acccgaaaca ggggagtgtt
    14341 cgtccgttat catacggttt tatttcgcag gatgacctgg caaaacagtt cctgaatgtc
    14401 tctgaagatt ttaaaccgaa tttttaacca aaggatatat atgcatacag gggatttgtg
    14461 tttttttaca ggtgccagcc tgtttatcgt gggggttgtt ggttatttct ttacaggacc
    14521 aaataataaa ggagtttatc tgctgagctc gtttgtcttc tttgtagtaa atgtcataag
    14581 tgtatgtatg ctgccagata atttcttgtt tctttgtggt gcggttatga atcagtgtgc
    14641 cgtaccggtt gtttcaatgg cagtcagtac catgccggtt atcacgaagt ttgacagttt
    14701 cagaaggatg tttgtttgta ttatgttgtc agccatatgg tctggagtga tgtggttttt
    14761 tattatttca ttagtgaaat gactggtgct aatttgtcat aaatgccaaa tccaatagtg
    14821 aggttgatta ccacagaaca gaggataaac cacactgcta tccagtgact tctgaataac
    14881 tcgcaaaaat aaacatagat gattagaaat agccatatca caacagcgat aatcgctata
    14941 aataacagta tcgtcacaaa taaatcagca aaggttttaa tggggaggga gtgcaataat
    15001 gaatacacca gtaaagcaaa agccgcatta cctgataatg actctcttat gatgtttctc
    15061 actatttcca gtttttcttt aaaagataac tttttcatat aacacactga ttaatttcac
    15121 gaaagttaag cagattatat acgtgaataa attcatgtaa accacaacag gatattcaaa
    15181 tgataagtaa acacagattc tctttacccc ggcttgatat tacgggaatg tgggtatttt
    15241 ccctgggtgt ctggtttcat atcgtcgccc gtcttgttta cagcaaaccc tggatggctt
    15301 tttttctggc ggagcttatt gcggccattc tcgtgctgtt cggcgcatac caggtgctgg
    15361 atgcatggat tgcccgtgtc agccgggaag agcgtgaggc actggaagcc aggcaacagg
    15421 ccatgatgga agggcggcag gaggggggac atgtctctca ctaaattact gccggtcatt
    15481 ctgttgatga tggccacagg tgtgcaggcc tccacccggg atgaaataga gcgactctgg
    15541 aatccacagg gtatggccgc gaagcctgca caaccggcag caggcgtgcc agccagaaca
    15601 gaaaagccgg ctccccgctg gttccgcctc agtaatggca ggcaggttaa cctggccgac
    15661 tggaaggtcg tgctgtttat gcagggacat tgcccttact gtcaccagtt tgacccggta
    15721 ctgaaacagc tggcgcaaca gtacggcttt tccgtttttc cctacaccct ggatggtcag
    15781 ggtgatacgg cctttccgga gacattgccg gtgccaccgg acgtgatgca gaccttcttc
    15841 ccgaatatcc cggtggccac accgaccacc ttcctggtca acgtcaacac gcttgaggca
    15901 ttaccgcttt tacagggggc aacggatgcc gccggtttta tggcgcggat ggataccgtt
    15961 ctgcagatgt acggaggaaa aaaaggtgcg aaataagcag gtggtgttac tcattgccgg
    16021 aatatccggc atcgcgacgg gaataatcgt cagcctgaat atccccttta tccgtcaggg
    16081 gcttttttat cccgccagcc ctgtagaaat tgtcgtttcg ctgtgtctta ccttttctgt
    16141 ttctgttgtg ttttttgtag gggcaattgt gggatggatt ttggtgtctg aaatatatta
    16201 cagccgcatg accggtctga atgaatcatc aggaatatca gaggaaactt atgatgacag
    16261 aaaacaaaaa gaatcaggaa ttcaaaatca gaaaaattaa gcgggatatt gagcgcggtt
    16321 gtgataatgc cgaaaaatac ttctggcttt ttgtggtttt ctttgttgcc ggcttgcttt
    16381 tatggcacgg tatgcatgct attttcagcg ccggtattga ttcgtggaaa gcgaacccgg
    16441 aattaaataa tttgatttat atgtggaaca tcctgatgta tgccattccg tatacgttat
    16501 atgcgcttgc tgccgggttt cttgttacat ttttttctgt ccccaatgta cgaattaata
    16561 ttcgggaata tcatgatatt cctgctgaaa cgccgtatgc gccgggagaa caaattaagg
    16621 gagggcagta ataatgcttc gcattaaacc tctttttatt ctttgtgccg ccttactgat
    16681 ggtgacgcct gcagcgtcag cggatgtgaa cagcgacatg aaccagttct ttaataagct
    16741 ggggtttgct tccaatacca cccagccggg cgtctggcag gggcaggccg ccggctatgc
    16801 ttacggtggc tccctgtatg cccgtactca ggtcaaaaat gttcagctga tttccatgac
    16861 gctgccggat atcaatgccg gatgtggcgg catcgacgcc tatctcggct cgttcagttt
    16921 tattaatggc gagcagctgc agcgttttgt taagcagatt atgagcaacg ctgccggtta
    16981 cttttttgac cttgccctgc agacgacggt gccggaaatc aaaaccgcaa aagattatct
    17041 gcagaagctg gcaagcgaca ttaacagcat gaacctcagc tcctgtcagg tggctcaggg
    17101 gcttgtgggt ggcctgttcc ctcagacaca ggaatctcag cggaaagtct gccaggacat
    17161 tggcagtgaa agcaatatct tcgccgactg ggccgcttcg cgtcaggggt gcactgtcgg
    17221 aggacagtcc tcctctgttc gtgacaaggc cagtgataag gataaggaac gggtgctgaa
    17281 aaaccagaat atcatatggg aggcactgaa aaagaacaag atgcttgacg gcaacaaaga
    17341 gctgaaagaa ttcatcatga cgctcaccgg tacgctcatt tttggggaag acagtgagat
    17401 tacccccctg ccggcgcgta ctactgaccg cgatatcctc cgggcaatta tggagggggg
    17461 aacggcaaaa atttaccact gtaacgattc ggacaagtgt ctgaaggtgg tggcggatgc
    17521 aaatgtgacc atcgcccggg ataaagccct gaaatcacag attacaaaac tgctcaccag
    17581 tattcagaat aaagcggtca gcgacacgcc tctggatgac agggaaaaag gcttcatttc
    17641 cagcaccacc atccccgtct ttaaatatct gattgacccg cagatgctcg gtgtgtccaa
    17701 cagcgtggtt taccagctga cggactatat cggctacgac atcatgctcc agtacattca
    17761 ggaactgctt cagcaggccc gggccatgat tgccaccggg aactatccgc aggctgtgat
    17821 ggacaatgtt ctggagaatc tgaatcaggc acagcaacag attgctgtct tccagagtca
    17881 ggtacaggtc cagcaggatg cactgctggt cgttgaccgt cagatgagtt acatgcgcca
    17941 gcagttgtcc gcccgcatgc tcagccgtta ccagaacaat tatcacttcg ggggaggcac
    18001 gctgtgaatg aagtctatgt tattgccggt ggtgagtggt tgcggaataa cctgaacgcc
    18061 attgccgctt ttatggggac ccggacatgg gattccatcg aaaaaattgc gctcacatta
    18121 tctgttctcg cggtggctgt gatgtgggta cagcggcaca acgtgatgga tttgctgggc
    18181 tgggtggccg tgtttgtgct tatcagcctg ctggttaatg tccgcacatc ggtgcagatt
    18241 attgataaca gtgacctggt ccaggttcac cgggtggaca atgtgccggt cggactggcg
    18301 atgccacttt cactgacgac acgtatcggg catgcaatgg tggtcagcta cgaaatgata
    18361 ttcactcaac cggacagcgt cacctacagc aaaacgggga tgctgttcgg ggcgaatctg
    18421 attgtgaaaa gcaccgattt cctgtcccgg aatccggaaa tcatcaatct gttccaggac
    18481 tacgtccaga actgcgtgct gggggatatt tacctgaacc acaaatacac gctggaagac
    18541 ctgatggcct cctctgaccc atacaccata atcttttctc agcccagccc tttacgtcag
    18601 gtgccgaata ataattacag ttttctgcac agctcagagc cgtttgtgtc ctgtaaggac
    18661 gcatccgtcg gactgaagga taagctgaat tttgatacga ataccggagg caagacctgg
    18721 cattattatg tgcagcagat atttggcggc agaccggacc ctgacctgtt attccgggaa
    18781 ctgctcagtg acagctacag ttatttctac ggtgccagcc agtcggccag ccagattatg
    18841 cgtaagaatg tgacaattaa cgcactgaaa gaaggtatta ccagtaatgc tgcccgcaat
    18901 ggtgacaccg ccagcctggt cagtctggcc actacctcat cgatggagaa acagcgtctg
    18961 gcacatgtct ccattggtca tgtaactatg cgaaacctgc cgatggtcca gactatcctg
    19021 acggggattg ccattggtat attcccgctc ctgatactgg cggctgtctt taacaagctg
    19081 acattgtctg tattaaaggg gtatgtgttt gccctgatgt ggctgcagac ctggccgttg
    19141 ttgtatgcca ttctgaacag tgccatgaca ttctatgcga aacagaatgg tgcgccggtc
    19201 gtgttgtctg aactctctca gatacagctg aaatactctg acctggcctc cactgctggg
    19261 tatctttcag ccatgatccc cccgttgtca tggatgatgg taaaaggcct tggcgcaggt
    19321 ttttccagcg tgtacagtca ctttgcctct tcctccatca gcccgactga cagtgccgca
    19381 ggcagtgtgg tggatggtaa ttactcctac ggcaacatgc agacggagaa cgtgaatggc
    19441 ttcagctgga gcaccaacag caccacgtca tttggccaga tgacgtatca gaccggcagt
    19501 ggtgcaacca ccacacagac ccgtgacggt aatatggtga tggatgcgag cggggcgatg
    19561 tcccggttac cggtcggtat caatgcaacg cgtcagattg cagcagcgca acaggaaatg
    19621 gcccgggagg cgtcgaacag agccgaaagc gccctgcatg gtttcagcag cagtattgcc
    19681 agtgcctgga acacgctcag tcagtttggt tctaaccggg ggagcagtga ttctgtcacc
    19741 ggtggtgctg acagcacgat gagcgcacag gattccatga tggccagccg tatgcgcagt
    19801 gcggtggaaa gctatgcgaa ggcgcataat atcagtaatg agcaggcgac aagagaactg
    19861 gcatcgagaa gcgcgactgc atcagctggg ttgtatggtg atgctcatgc cggattagat
    19921 gcaaagccca gcctttttgg tgtgcgtgtt gatgttggcg ctaaggttgg tgggcgagcc
    19981 agcattgacg gtagtgatct tgactcacat gaagccagca gtggttcacg ggccagccat
    20041 gatgcacgtc atgatatcga tgctaaagcc agtaaggact ttaaagaggc cagcgattac
    20101 tttaccagtc gcaaggtcag tgagtccggc agccatactg acaataatgc tgattcccgg
    20161 gtggaccagt tgtctgcagc cctgaactca gcgaaacaga gttacgacca gtacacgacg
    20221 aatatgacct gcagccatga atatgcggaa atggcctccc gtactgaaag catgagcggg
    20281 cagatgagtg aagacctgtc gcaacagttt gcacagtatg tgatgaaacg cgcaccgcag
    20341 gatgcagaag ctattctgac aaataccagc tcaccggaga ttgcagaaca ccggcgggcg
    20401 atggcctgga cttttgttca ggaacaggtg cagcctggtg ttgataatgc ctggcgtgaa
    20461 tcccgtgggg acatcggtaa aggaatggag agcgtacctt cgggcggcgg cagccaggat
    20521 attattgctg accatcaggg acatcaggcc attattgagc aaagaacgca ggacagtaat
    20581 atccgtaatg atgtaaaaca tcaggttgat aatatggtca cagaatatcg gggcaatatc
    20641 ggagatactc agagcagtat ccgtggcgag gaaaatattg taagagggca gtattctgaa
    20701 ctgcaaaatc atcataaaac agaggcatta tctcagaata ataaatataa tgaagagcga
    20761 gaaagtcaaa agataatacc tgagccatca gaagaggctg tgaaaaaaat ggttgatgat
    20821 aaaaaggata gactgaaggg gccattatga tcactcacta tgatataaaa acggagacac
    20881 agaaactaaa ggatgtactc tctgtagagg gggttaatat tccgccatta ttacaagtca
    20941 taaaaccagg ggggtatgta tttttatggg ttttattatg gccgacattt cttcgtttgc
    21001 tggcagataa agttgatatc cgagatgctg gatttgatat atgttttagt ggcgtaatgg
    21061 gttttatcct ttttgttgcc attactaatg gaatgatgct gtatcttgct atccccaaaa
    21121 aattcagaga tgagtcgaaa gtcatatctt tcatgtatga taagaataaa acgtatattt
    21181 tgctattttt gattgctttt tcaatggttt catttgccca tacactctta tatgagtttt
    21241 tattgattgc tttgtttata atctttttct ttatttatgc tatagacata aatcgttata
    21301 acctctccgc aatagtttca gtaataggac tgttcaaaaa agagtctgtt agttaaaagg
    21361 atattctgga tgaaaacaaa aaaattaatg atggttaccc tggtcagttc cacgctggcc
    21421 ctttcagggt gtagtagcgt tggcaatata aaacatgtgg aaccatctaa atacgtacat
    21481 gttacggact atttaaatga tgcttttgaa aaagcaaatt gcgttggctc tattttcctt
    21541 tcgcagggaa ggatatttac tgatgctgat ggactgaagt atcagttgaa ttggccaaat
    21601 accaagtgtt cctctaattc tatcgagtat gctgtagaaa aatattgtat tcataaaggg
    21661 gggaagatgg tacaggaggg acggtggtgt aaggtaaatg gcgagccatt attctatgtt
    21721 gattatggca gtaattggaa acattatggt gaaaatttta tggctgtgga acaagggagt
    21781 tttcagggaa gacagcaatg gttaaatgct gctgagcgac ttggattttt gagtaaacgt
    21841 gtccagttac aacaaaagct agaagatgaa atcaatgcaa aaaaagaagc tgccgaatta
    21901 caacgaaaaa tgaaagaaaa aaacacacct gtgaatgcag atgttgggga tttgatttgt
    21961 aaagaagatt atgaagctaa accatatcaa tatcccggga ctgcttatta taaagcatat
    22021 gttgaaaaaa aagaaaaaaa taaacttcag cttagactag tttggcatgg tggtaatggc
    22081 ttcgttatta atgatattac gccttcgaat aacattatat ggtcatcgcc aaacggctgg
    22141 aaacattgta attgagtagt aagctgacgc ctcgcgttgc tcgttgtcca accccgggac
    22201 gtaacaaaat aaattttgtc acctcccggc gtttccgaaa taaatatgaa aacacttcac
    22261 ggttgtgcaa attctttgta cgaccgtgaa gtgttgccgg cgaagccgga atttaacggt
    22321 tatta
//
LOCUS       DAKISA010000073        21998 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.073, whole genome shotgun sequence.
ACCESSION   DAKISA010000073 DAKISA010000000
VERSION     DAKISA010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21998
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.073"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..989)
                     /gene="cmtA"
                     /locus_tag="OVW45_002800"
     CDS             complement(<1..989)
                     /gene="cmtA"
                     /locus_tag="OVW45_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HCV5527204.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     LVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWVFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVATLVSFA"
     gene            complement(1017..1460)
                     /gene="cmtB"
                     /locus_tag="OVW45_002801"
     CDS             complement(1017..1460)
                     /gene="cmtB"
                     /locus_tag="OVW45_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HCV5527205.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTAASEKQLADIISRG"
     gene            complement(1774..3765)
                     /gene="tkt"
                     /locus_tag="OVW45_002802"
     CDS             complement(1774..3765)
                     /gene="tkt"
                     /locus_tag="OVW45_002802"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCV5527206.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            4043..4801
                     /gene="loiP"
                     /locus_tag="OVW45_002803"
                     /gene_synonym="yggG"
     CDS             4043..4801
                     /gene="loiP"
                     /locus_tag="OVW45_002803"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCV5527207.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(4944..5864)
                     /gene="speB"
                     /locus_tag="OVW45_002804"
     CDS             complement(4944..5864)
                     /gene="speB"
                     /locus_tag="OVW45_002804"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCV5527208.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLTFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(6002..7978)
                     /gene="speA"
                     /locus_tag="OVW45_002805"
     CDS             complement(6002..7978)
                     /gene="speA"
                     /locus_tag="OVW45_002805"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCV5527209.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(7987..8118)
                     /gene="yqgB"
                     /locus_tag="OVW45_002806"
     CDS             complement(7987..8118)
                     /gene="yqgB"
                     /locus_tag="OVW45_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCV5527210.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            8254..8469
                     /locus_tag="OVW45_002807"
     CDS             8254..8469
                     /locus_tag="OVW45_002807"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5527211.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            8773..9927
                     /gene="metK"
                     /locus_tag="OVW45_002808"
     CDS             8773..9927
                     /gene="metK"
                     /locus_tag="OVW45_002808"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCV5527212.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            10363..11757
                     /gene="galP"
                     /locus_tag="OVW45_002809"
     CDS             10363..11757
                     /gene="galP"
                     /locus_tag="OVW45_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCV5527213.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFADKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            11834..12331
                     /locus_tag="OVW45_002810"
     CDS             11834..12331
                     /locus_tag="OVW45_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCV5527214.1"
                     /translation="MKTSRLPIAIQQAVMRGLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            12426..13133
                     /gene="endA"
                     /locus_tag="OVW45_002811"
     CDS             12426..13133
                     /gene="endA"
                     /locus_tag="OVW45_002811"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCV5527215.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            13213..13944
                     /gene="rsmE"
                     /locus_tag="OVW45_002812"
     CDS             13213..13944
                     /gene="rsmE"
                     /locus_tag="OVW45_002812"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCV5527216.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            13957..14907
                     /gene="gshB"
                     /locus_tag="OVW45_002813"
     CDS             13957..14907
                     /gene="gshB"
                     /locus_tag="OVW45_002813"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCV5527217.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            15016..15579
                     /locus_tag="OVW45_002814"
     CDS             15016..15579
                     /locus_tag="OVW45_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCV5527218.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            15579..15995
                     /gene="ruvX"
                     /locus_tag="OVW45_002815"
     CDS             15579..15995
                     /gene="ruvX"
                     /locus_tag="OVW45_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCV5527219.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(16170..17150)
                     /locus_tag="OVW45_002816"
     CDS             complement(16170..17150)
                     /locus_tag="OVW45_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCV5527220.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSSKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQKREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQRVGEGRL"
     gene            17168..17872
                     /gene="yggS"
                     /locus_tag="OVW45_002817"
     CDS             17168..17872
                     /gene="yggS"
                     /locus_tag="OVW45_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCV5527221.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            17890..18456
                     /gene="yggT"
                     /locus_tag="OVW45_002818"
     CDS             17890..18456
                     /gene="yggT"
                     /locus_tag="OVW45_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCV5527222.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            18453..18743
                     /locus_tag="OVW45_002819"
     CDS             18453..18743
                     /locus_tag="OVW45_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCV5527223.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQGNSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            18751..19344
                     /locus_tag="OVW45_002820"
     CDS             18751..19344
                     /locus_tag="OVW45_002820"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCV5527224.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARYAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            19337..20473
                     /gene="hemW"
                     /locus_tag="OVW45_002821"
     CDS             19337..20473
                     /gene="hemW"
                     /locus_tag="OVW45_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCV5527225.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            20784..21770
                     /locus_tag="OVW45_002822"
     CDS             20784..21770
                     /locus_tag="OVW45_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HCV5527226.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSEGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQKQILETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
     gene            21815..>21998
                     /locus_tag="OVW45_002823"
     CDS             21815..>21998
                     /locus_tag="OVW45_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HCV5527227.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLG"
BASE COUNT         5442 a         5716 c         5600 g         5240 t
ORIGIN      
        1 atagcaaagg acaccagggt agctaccgta acaccggcaa ttgtcgccag gaacgagcct
       61 ttcggcgtca gtgccaggta agcaaagata gaccccggac ttgggccagc caccagacca
      121 ccgtccagta agttaaacac ccaggtgcca gacataccgc ccgcaatcat ggcaataatg
      181 gtcagcggct tcatcagcac atacgggaaa tacagttcgt ggatcccacc gaggaagtga
      241 ataatcatcg ccccgggcgc agaacgttta ctcatccctt taccaaacaa ggtaaacgcc
      301 agcagcagcc ctaagccagg cccaggattg gacgccacca taaagaagat ggatttaccg
      361 ttaaccgaag cctgttgcat tcccagcgga taatagacgc cctgatcgat cgcattattg
      421 aggaaaagca ctttcgccgg ttcattgatg actgacagca gaggcagata acccgcatgt
      481 accagcgcct caatacactc tttgacgaga gtattggcaa ttaacaccgc cgggccgata
      541 acttcaaaac ccagcagaca aaggagcatc cccgcgatac ctaatgagaa gttattgata
      601 accatctcaa aaccggcagg tatgcgtttt tccagtgcct tatcaacata ttttatgacc
      661 agcccaccga gcggccccat aatcattgag ccaaggaaca tcgggatctc tgcgccgacg
      721 atcacaccaa tagtacctat tccgcccatg accgcgccgc gtttaccgcc gaccagatga
      781 ccaccggtag aaccaatcat cacgggcaat aaataggtaa tcatcgggcc gacaattttg
      841 gcgaaatgtt cgttaggcag ccaaccggtg ggaataaata acgcagtaat aaaaccccag
      901 gcaataaaag caccaatatt ggggatgacc attgccgtca aaaagccccc aaaagcctgg
      961 acctttgcac gagcagactt gttttccata atattatcct gtagaggaga gagtaattat
     1021 ccgcggctga ttatgtccgc taattgtttt tctgatgctg ctgttaagag ttgttcgagt
     1081 atttcttctt cgcacagtaa ttcacttaac gcctgaatag cgccaatgtg cgaatcggca
     1141 tcggcagcag aaaggccgat gagtaatttt attggttcgt cattccccgg aaaataaaca
     1201 ccttgttcaa gtaatgtcaa agacatcccg gttttaagcg ccccacattc cggtcgcgca
     1261 tgaggcattg ccacgcctgg tgcgagaata taataagggc cattgttaat ggtggaatct
     1321 ttaattgcct gaatgtaatt ctcgctgata tagtttttat ccagcaatga taccatcgag
     1381 aaatcgatag cttcctgcca atcttttgct gaatgtataa ctgagattga tgattctgga
     1441 aaatagtcac taagccgcat aaattatcct tattttattt ttagggtacc gtccggcggt
     1501 aaaaataaaa tactcgtgcg gtttaatgaa agcaattgag cagggaaacc ggtgttttga
     1561 aaacagtgaa ttattaattt cttatataac attgagttat agatataaca acaaatgtca
     1621 atacgcatat cgtgatgcat attacataat tgatatttat tgcatataaa tatttgtgat
     1681 ctacaacacg ctttatctat tgcttgtccg caaacggaca tatcaaggta ataaaaaagg
     1741 tcgccgaagc gaccttttta cccgaaatgc taattacagc agttcttttg ctttcgcaac
     1801 gacgttgtcg acggtgaagc cgaactcttc gaacagcaac tctgccggag ctgactcacc
     1861 gaaggtggtc ataccgatga tagcgccatt caggccaaca tacttgtacc agtagtcagc
     1921 aatacccgct tctaccgcaa cgcgtgcagt aaccgctttc ggcagtacgg attcacggta
     1981 agccgcatcc tgcttgtcga atgcatcggt agacggcatg gaaaccacgc gcgccttcac
     2041 gccttcggca gtcagttttt cgtaggcggc aacagccagt tcaacttccg aaccggtcgc
     2101 gatgaagatc agttccggct gaccagcaca gtctttcagc acataaccac cgcgcgcgat
     2161 gtttgccagt tgctcttcag tacgttcctg ctgcgccaga ttctgacggg agaggataag
     2221 cgcggtcgga ccgtcctgac gctcaacacc gtatttccac gcgaccgcgg attcaacctg
     2281 gtcacacgga cgccatgtag acatgttcgg ggtcacgcgc agagaagcga cctgctcaac
     2341 cggctggtga gtcgggccgt cttcgcccag accgatggag tcgtgggtgt aaaccatcac
     2401 ctgacgctgt ttcatcagcg cagccatacg tacagcgtta cgtgcgtatt cgacaaacat
     2461 caggaaggtg gaggtgtacg gcaggaagcc accgtgcagg gagataccgt tagcaatcgc
     2521 ggtcataccg aactcgcgaa caccgtagtg gatgtagtta cccgcagcat cttcgttgat
     2581 tgctttagaa ccagaccaca gggtcaggtt agacggtgcc aggtcagcgg agccgccgag
     2641 gaattccggc aacagcgggc cgaacgcttc aatagcattc tgagacgctt tacggctggc
     2701 gattttcgcc ggattagcct gcagtttagc gataaactct ttcgctttgg cgtcgaagtc
     2761 agacggcatt tcgcctttca tacggcgggt aaattcagcc gcttcctgcg gataagcttt
     2821 cgcgtaagcc gcaaacttct cattccatgc agattctttc gcctggcctg cttctttcgc
     2881 atcccactgc gcatagattt cagacgggat ttcgaacggt gcgtatttcc agcccagctg
     2941 ttcgcgggtc agagcgattt ctgcatcacc cagcggcgca ccgtgggagt cgtgagtacc
     3001 ggctttgttc ggggaaccga aaccgatgat ggttttgcac atcagcaggg acggtttgtc
     3061 agtcactgcg cgcgcttctt ctactgcgcg tttgatggat gctgcgtcat gaccgtcgat
     3121 gtcgcgaata acgtgccagc cgtaagcttc gaaacgcatt gcggtgtcgt cggtgaacca
     3181 gccttcaacg tgaccatcaa tagagatacc gttgtcatcg tagaacgcaa tcagtttacc
     3241 cagcttcagc gtacccgcca gagagcaaac ttcgtgggag atgccttcca tcatgcagcc
     3301 gtcgcccatg aaggcgtagg tgtagtggtc gacaatgtcg tggcccggac ggttaaactg
     3361 cgccgccagc gttttttctg caatcgccat accgactgcg ttggcaatac cctgacccag
     3421 cggaccggtg gtggtttcca caccagcggt ataacctact tccgggtggc ccggagtttt
     3481 agagtgcagc tgacggaagt ttttcagttc ttccatcggc agatcgtaac cggtgaggtg
     3541 cagcaggctg tagatcagca tggagccgtg gccgttggac agcacgaagc ggtcacggtc
     3601 agcccaggac ggattctgcg ggttgtgttt caggaaatca cgccacagga cttcggcaat
     3661 gtcagccata cccatagggg cacccgggtg accggatttg gctttctgta ctgcgtccat
     3721 gctcagcgca cgaatagcat tggcaagctc tttacgtgag gacattttga ctccagatcg
     3781 gatgatgaag ggcacgccct taacgacttg acgacagcgc gttttgggct acgccggaaa
     3841 atttgccaac aatttaccgc aagccgcgcg tcatgtacat ggaacatcct tttgccgctt
     3901 cagaaatctc tggatcatgc tcgcatgttg cgcaatctac tcgcccgtcc gctgcgcttt
     3961 tccttatact gagactgagc gtcgattcac ctgcaaacgg cgcattttta gaataatcct
     4021 gaccttgtgc ggaagagaaa acatgaaaat tcgcgcctta ttggtagcaa tgagcgtggc
     4081 aacggtactg actggttgcc agaatatgga ctccaacgga ctgctctcat caggagcgga
     4141 agcttttcag gcttacagtt tgagtgatgc gcaggtgaaa accctgagcg atcaggcatg
     4201 tcaggagatg gacagcaagg cgacgattgc gccagccaat agcgaatacg ctaaacgtct
     4261 gacaactatt gccaatgcgc taggcaacaa tatcaacggt cagccggtga attacaaagt
     4321 gtatatggcg aaggatgtga acgcctttgc aatggctaac ggctgtatcc gcgtctatag
     4381 cgggctgatg gatatgatga cagataacga agtcgaagcg gtgatcggtc acgagatggg
     4441 gcacgtggcg ttaggccatg tgaagaaagg gatgcaggtg gcgcttggta caaatgccgt
     4501 gcgagtagct gcggcctctg cgggcgggat tgtcggcagt ttatctcaat cacaacttgg
     4561 tgatctgggc gagaaattag tcaattcgca attctcccag cgccaggaag cagaagccga
     4621 tgattattct tacgatcttc tgcgccaacg cggcatcagc ccggcaggtc ttgccaccag
     4681 ctttgaaaaa ctggcgaaac tggaagaagg ccgccaaagc tcaatgtttg acgaccatcc
     4741 ggcatccgcc gaacgcgccc agcatattcg cgatcgcatg agcgcggatg ggattaagta
     4801 aagcctggtg gtgtcgaacg taggtcagat aaggcgttca cgccgcatct gacatcaatg
     4861 gcacatttgt taccctgtgc gcattgccgg atgcgatgct gactgcatct tatccggcct
     4921 acgggtggca tcgcatctgg tgcttactcg ccctttttcg ccgcttggat atacagcatc
     4981 tccagcgcca gcgtcgccgc agccagagcg gtgatttccg actgatcgta tgccggagcc
     5041 acttccacta cgtccatccc aacaatgttg agatctttca ggccgcgtac cagtttaata
     5101 gcgcgatcgg aggtcaggcc gccaatcact ggcgtaccgg tgcctggtgc aaaagcagga
     5161 tccaggcagt cgatatcaaa agtcaggtaa accggcatat cacccacaat ctgtttcact
     5221 tgggcgataa cgtcatccac gctgcgatcg ttcacctggc aggcgtccag cacggtaaag
     5281 ccgttgtctt tatcaaactc ggtacgaata ccaatctgca cggaatgatt cgggtcgatc
     5341 agaccttctt tcggcgcggt atagaacata gtgccgtggt caaattcaca accgttcgca
     5401 taggtatcgg tgtgggcgtc aaagtgtacc agcgccattt tgccgaaatg cttcgcatga
     5461 gcacgcagca gcggcagcgt aacaaagtgg tcaccaccga aggtgagcat acgcttaccg
     5521 gcagccagca gcttctcggc gtgcgcctgc agcttttcgc tcatctcacg ggcatcgcca
     5581 aaggcatata ccagatcgcc gcagtccacg acgttcagac gctcacgcat gtcgaaattc
     5641 cacgggaagc ggttgtgttc ccaggccaga ttcgtcgaaa cctgacggat cgctgccgga
     5701 ccgtggcgac cacccgcacg accagaagtg gccatatcga acggcacgcc agtaatcacc
     5761 cagtctgcat cgctgtcata cggctggaag ttcatcggca ggcgtaaaaa accaaaggca
     5821 ttggaaacca gtgagttatc gtattgatga cctaaggtgc tcatggatgg actcctatta
     5881 caaagtcgat ataaaaaaac ccttccgcgt cgttaggccc gacgaggaag ggttggattt
     5941 gtcacaataa attgtggcgg attatcaccg ctaaattaag cggattcaag taacacagga
     6001 cttactcatc ttcaagataa gtataaccgt acaaacctgc ctcgaactct tcaaggaact
     6061 gttgttgcag ttcagcatca agatcggttt tcttcacttg atcgcggaac tgggttaaca
     6121 gcgttttcgg atcgagctgt acatattgca gcatgtccgc cacggtatcg ccttcgtcag
     6181 acagttctac ttctacgcta ccgtcaggga acacgaacac gtcaaccgct tcggtatcac
     6241 cgaacaggtt gtgcatattg ccaaggatct cctgatatgc gccgaccata aagaaaccga
     6301 gcatcggcgg attctctgga tcgtactccg gcattggcat tgtcgtggca ataccatcac
     6361 catcaatata gtggtcgata gcaccgtcag agtcacaggt aatatccagc agcacagcgc
     6421 gacgttccgg cacttgatcc agcccttcca gcggcagaac cgggaacaac tggtcgatcc
     6481 cccatgcgtc cggcattgac tggaacagcg agaaattgac gtacattttg tccgccatac
     6541 gttcctgcag ttcgtcgatt atcggacgat gcgcacggtt ttgcggatcc agttgcttct
     6601 gcacttcatg gcacatgctc agatagagtt gttctgccca tgcacgttct tgcaggctaa
     6661 aggtgccgga agagtagccg atatgaatgt cgtgcagatc catctggctg tcgtgtaacc
     6721 attcacgcaa agaacggcga gttcccggtt cgtgcatttc ctgccaggtt tcccacatgc
     6781 tttgcagcgc gcgcggcgca tcttctgcgg gcgcggtcgg caccgtatat tcgttacgtt
     6841 ccacgccgat gatattcgac accagcacgg tatggtgcgc agtcaccgca cggcccgatt
     6901 cggtgattac cgtcggatgc ggcagaccat tttcttcaca cgcatcgcca atcgcccaga
     6961 tgatgttgtt ggcgtattca ttgaggccat agtttacgga gcagtcagac tgcgaacggg
     7021 tgccttcata gtccacgccc agaccgccgc cgacgtcgaa gcactgaata ttgacgccca
     7081 gcttgtgcag ttccacatag aaacgcgcgg attcacgaac gcccgtggcg atatcgcgaa
     7141 tattcgccat ctgcgaaccg aggtggaagt gcagtagttg caggctgtcg agacgcccgg
     7201 cttcacgcag ggtttcaacc agttgcagta cctgagtcgc agccaggccg aacttcgatt
     7261 tttccccgcc ggaggactgc catttacccg agccctgcga agccagacgt gcacgcacgc
     7321 ccagacgagg aacgacattc agacgttctg cttcatccag cacaatggcg atttctgaca
     7381 tcttctcaat gaccagatag accttgtgcc ccatcttctc gccaattaat gccaggcgga
     7441 tatattcgcg gtctttataa ccgttgcaga cgatgacgct acgggtcatg ccagcatgtg
     7501 ccagtactgc catcaactcg gctttggaac cggcttccag acccagcggt tcgcccgaat
     7561 gaatcaggga ctcaatcacg cggcggtgct ggttaacttt gatcggataa acaaggaagt
     7621 aatcgccgtt atagccgtag gattccctcg cacgtttgaa cgcggcgtta atggaacgca
     7681 aacggtgctg caggatctgt gggaaacaga acagtgcagg cagacgctga ccctgtgctt
     7741 cacgagtttt cactaactgc gcgagatcga cgcgagcttc cgggacgtcc gggtccgggc
     7801 acacgctgat gtggcccagc tcgttaacgt catagtagtt attgccccac caggcaatat
     7861 tgtaagtacg cagcatcttg ctggcttcct gggagctcat tgcaacctcc tgcatggagc
     7921 gtagtacacc gtgttcgccc gctgacgaag gcaaacccat agacatgtcg tcagacatag
     7981 cgaacctcaa attattttat taagtgtaaa acagttaacg actatcgcag cctggaactg
     8041 cgataacaac ccataagcac atggattttc cagcagtgaa tgctgacgct ccaactgcgc
     8101 aaccggtttc tttttcataa cattattaag cacataaccg aacgtaagtg tgacagttcg
     8161 gcgaaaccac gagaaaactc ttgtttttac aagagcgccc ttgttcagtc ctcagtaact
     8221 gtaaccagct cttgaatcct gagaagcgcc gagatgggta taacatcggc aggtatgcaa
     8281 agcagagatg cagagtgcgg ggaacgaatc ttcaccagaa cggtgagaca ggttaagcag
     8341 cagacaacgg ttcattattt cgtatcacct ccacggccgc ctgttaagac gaacccacaa
     8401 gccaaaactc tgatttcaac ccggctggaa gtggcaacac gaaagaaacg tcgtgtgctt
     8461 tttatttaag ccgcgcgccg cgttttatac cccacaatgg cagaaaattg caaaagataa
     8521 atacgcagaa tgccggcatt gtcaggaaaa atttccagcc acgtttttaa cagaatgaga
     8581 cacgattcaa aaaaaagtgg aaatcgggtg aagaattgac ctaaaatagc catccagatg
     8641 ttaatccatc cataccgatt aacactcaga ctgccagtgt ttttaacctg cagagtcgtg
     8701 gtaggatccg ctaccacaga aaatccacac aacagtttga gctaaccaaa ttctctttag
     8761 gtgatattaa atatggcaaa acaccttttt acgtccgagt ccgtctctga agggcatcct
     8821 gacaaaattg ctgaccaaat ctctgatgcc gttttagacg cgatcctcga acaggatccg
     8881 aaagcacgcg ttgcttgcga aacctacgta aaaaccggca tggttttagt tggcggcgaa
     8941 atcaccacca gcgcctgggt agacatcgaa gagatcaccc gtaacaccgt tcgcgaaatt
     9001 ggctatgtgc attccgacat gggctttgac gctaactcct gtgcggttct gagcgctatc
     9061 ggcaaacagt ctcctgacat caaccagggc gttgaccgtg ccgatccgct ggaacagggc
     9121 gcgggtgacc agggtctgat gtttggctac gcaactaatg aaaccgacgt gctgatgcca
     9181 gcacctatca cctatgcaca ccgtctggta cagcgtcagg ctgaagtgcg taaaaacggc
     9241 actctgccgt ggctgcgccc ggacgcgaaa agccaggtga ctttccagta tgacgacggc
     9301 aaaatcgttg gtatcgatgc tgtcgtgctt tccactcagc actctgaaga gatcgaccag
     9361 aaatcgctgc aagaagcggt aatggaagag atcatcaagc cgattctgcc cgctgaatgg
     9421 ctgacttctg ccaccaaatt cttcatcaac ccgaccggtc gttttgttat cggtggcccg
     9481 atgggtgact gcggtctgac tggtcgtaaa attatcgttg atacctacgg cggcatggca
     9541 cgtcacggtg gcggagcatt ctctggtaaa gatccatcaa aagtggaccg ttccgcagcc
     9601 tacgcagcac gttatgtcgc gaaaaacatc gttgctgctg gcctggccga tcgttgtgaa
     9661 attcaggttt cctacgcaat cggcgtggct gaaccgacct ccattatggt agaaactttc
     9721 ggtactgaga aagtgccttc tgaacaactg actctgctgg tacgtgagtt cttcgacctg
     9781 cgcccatacg gtctgattca gatgctggat ctgctgcacc cgatctacaa agaaaccgca
     9841 gcatacggtc actttggtcg tgaacatttc ccgtgggaaa aaaccgacaa agcgcagctg
     9901 ctgcgcgatg ctgccggtct gaagtaatct ttcttcacct gctttcaaag gccagcctcg
     9961 cgctggcctt tttcttttac ctcaccgcgg tggataggca ttcacgccgc atccggcaaa
    10021 aagctgcccg cacaataaca tcattcttcc tcaacacgtt ttatcgcaaa ttatcatcac
    10081 tactgaaacc gattacacca ataacaccag ccaaatattt gtaatatttt catattatta
    10141 ttcagttttc acagttgtta catttctttt cagtaaagtc ttaattgcag ataacagcgt
    10201 ttaatctatg atgatataac tcaattattt tcatgcactt aaaccataac taagataaat
    10261 gttagtgtaa gcgattacac tgatgtgatt tgcttcacat ctttttacgt cgtactcacc
    10321 tatcttaatt cacaataaaa aataaccata ttggagggca tcatgcctga cgctaaaaaa
    10381 caggggcggt caaacaaggc aatgacgttt ttcgtctgct tccttgccgc tctggcggga
    10441 ttactctttg gcctggatat cggtgtaatt gctggcgcac tgccgtttat tgcagatgaa
    10501 ttccagatta cttcgcacac gcaagaatgg gtcgtaagct ccatgatgtt cggtgcggca
    10561 gtcggtgcgg tgggcagcgg ctggctctcc tttaaactcg ggcgcaaaaa gagcctgatg
    10621 atcggcgcaa ttttgtttgt tgccggttcg ctgttctctg cggctgcgcc aaacgttgaa
    10681 gtactgattc tttcccgcgt tctactgggg ctggcggtgg gtgtggcctc ttataccgca
    10741 ccgctgtacc tctctgaaat tgcgccggaa aaaattcgtg gcagtatgat ctcgatgtat
    10801 cagttgatga tcactatcgg gatcctcggt gcttatcttt ctgataccgc cttcagctac
    10861 accggtgcat ggcgctggat gctgggtgtg attatcatcc cggcaatttt gctgctgatt
    10921 ggtgtcttct tcctgccaga cagcccacgt tggtttgccg acaaacgccg ttttgttgat
    10981 gccgaacgcg tgctgctacg cctgcgtgac accagcgcgg aagcgaaacg cgaactggat
    11041 gaaatccgtg aaagtttgca ggttaaacag agtggctggg cgctgtttaa agagaacagc
    11101 aacttccgcc gcgcggtgtt ccttggcgta ctgttgcagg taatgcagca attcaccggg
    11161 atgaacgtca tcatgtatta cgcgccgaaa atcttcgaac tggcgggtta taccaacacc
    11221 acagagcaaa tgtgggggac agtgattgtc ggcctgacca acgtacttgc cacctttatc
    11281 gcaatcggcc ttgttgaccg ctggggacga aaaccaacgc taacgctggg cttcctggtg
    11341 atggctgctg gcatgggcgt actcggtaca atgatgcata tcggtatcca ctctccgtcg
    11401 gcgcagtatt tcgccatcgc catgctgctg atgtttattg tcggttttgc catgagtgcc
    11461 ggtccgctga tttgggtact gtgctccgaa attcagccgc tgaaaggccg cgattttggc
    11521 atcacctgct ccactgccac caactggatt gccaacatga tcgttggcgc aacgttcctg
    11581 accatgctca acacgctggg taacgccaac accttctggg tgtatgcggc tctgaacgta
    11641 ctgtttatcc tgctgacatt gtggctggta ccggaaacca aacacgtttc gctggaacat
    11701 attgaacgta acctgatgaa aggtcgtaaa ctgcgcgaaa tcggcgctca cgattaatct
    11761 cctcaggctt cctcccgtcg cggaggaagc cgcctcttgc agtcatcttt tcttcgctct
    11821 atcctctgcc gctatgaaaa catcccgtct ccctatcgcc atccaacagg ccgttatgcg
    11881 cggcctgcgg gaaaaactcg cccaggccaa cctgaagcta gggcgtaact acccggagcc
    11941 aaaactctct tacacccagc gcggaacctc cgccggaaca gcctggctgg aaagctatga
    12001 aatccgcctc aatcccgttt tgctgttgga aaacagtgaa gcttttattg aagaagtggt
    12061 accgcacgaa ctggcacatt tgctggtatg gaaacatttc ggccgcgtag cgccacatgg
    12121 caaagagtgg aagtggatga tggaaagcgt gctgggtgtt cccgcccgtc gtacgcatca
    12181 gttcgaactg caatccgtgc gtcgcaacac cttcccctac cgctgcaagt gccaggagca
    12241 tcagcttacc gtacgccgcc ataatcgcgt agttcgtggc gaggccgtct atcgctgtgt
    12301 tcactgcggt gaacagctgg ttgcgaaata accatctgaa ctatcaggaa ctttcctgat
    12361 ctggctgatt gcataccaaa acaactttcg ctacgttgct ggctcgtttt aacacggagt
    12421 aagtgatgta ccgttatttg tctattgctg cggtggtact gagcgcagca ttttccggcc
    12481 cggcgttggc cgaaggtatc aatagttttt ctcaggcgaa agccgcggcg gtaaaagtcc
    12541 acgctgacgc gcccggtacg ttttattgcg gatgtaaaat taactggcag ggcaaaaaag
    12601 gcgttgttga tctgcaatcg tgcggctatc aggtgcgcaa aaatgaaaac cgagccagcc
    12661 gcgtagagtg ggaacacgtc gttcccgcct ggcagttcgg tcaccagcgc cagtgctggc
    12721 aggacggtgg acgtaaaaac tgcgctaaag atccggtcta tcgcaagatg gaaagcgata
    12781 tgcataacct gcagccgtca gtcggtgagg tgaatggcga tcgcggcaac tttatgtaca
    12841 gccagtggaa tggcggcgaa ggccagtacg gtcaatgcgc catgaaggtc gatttcaaag
    12901 aaaaagctgc cgaaccacca gcgcgtgcac gcggtgccat tgcgcgcacc tacttctata
    12961 tgcgcgacca atacaacctg acactctctc gccagcaaac gcagctgttc aacgcatgga
    13021 acaagatgta tccggttacc gactgggagt gcgagcgcga tgaacgcatc gcgaaggtgc
    13081 aaggcaatca taacccgtat gtgcaacgcg cttgccaggc gcgaaagagc taacctacac
    13141 tagcgggatt ctttttgtta acccctaccc cacgcgtaca accgcgtggg gagacgacgc
    13201 ggatttttaa ctatgcgtat cccccgcatt tatcatcctg aaccactgac cagccattct
    13261 cacatcgcgc tttgcgaaga tgccgccaac catatcgggc gcgtactgcg catggggccg
    13321 gggcaggcgt tgcaattgtt tgacggtagc aaccaggtct ttgacgccga aattaccagc
    13381 gccagcaaaa aaagcgtgga agtgaaggtg ctggaaggca agatcgacga tcgcgaatct
    13441 ccgctgcata ttcacctcgg tcaggtgatg tcgcgtggtg aaaaaatgga atttacgatc
    13501 cagaaatcga tcgaactcgg tgtaagcctc attacgccac ttttttctga gcgctgcggc
    13561 gttaaactgg atagtgaacg tctgaacaag aagctccagc agtggcagaa gattgcaatt
    13621 gctgcctgtg agcagtgtgg tcgtaaccgg gtgccggaaa tccgtccagc gatggatctg
    13681 gaagcctggt gtgcagagca ggatgaagga ctgaaactga atcttcaccc gcgcgccagt
    13741 aacagcatca atacgttgcc gttaccggtt gaacgcgtcc gcctgctgat tggcccggaa
    13801 ggcggtttat cggcagatga aattgccatg actgcccgct atcaatttac tgatatcctg
    13861 ttgggacctc gcgttttgcg tacagagaca actgcgctca ccgccattac cgcgctacaa
    13921 gtacgatttg gcgatttggg ctaacggaga agaataatga tcaagctcgg catcgtgatg
    13981 gaccccatcg caaacatcaa catcaagaaa gattccagtt tcgctatgtt gctggaagca
    14041 cagcgtcgtg gttacgaact tcactatatg gagatgggcg atctgtatct gatcaatggc
    14101 gaagcccgcg cccatacccg cacgctgaac gtgaagcaga actacgaaga gtggttttcg
    14161 ttcgtcggtg aacaggatct gccgctggcc gatctcgatg tgatcctgat gcgtaaagac
    14221 ccgccgtttg ataccgagtt tatctacgcg acctatattc tggaacgtgc cgaagagaaa
    14281 gggacgctga tcgttaacaa gccgcagagc ctgcgcgact gtaacgagaa actgtttacc
    14341 gcctggttct ctgacttaac gccagaaacg ctggttacgc gcaataaagc gcagctaaaa
    14401 gcgttctggg agaaacacag cgacatcatt cttaagccgc tggacggtat gggcggcgcg
    14461 tcgattttcc gcgtgaaaga aggcgatcca aacctcggcg tgattgccga aaccctgact
    14521 gagcatggca ctcgctactg catggcgcaa aattacctgc cagccattaa agatggcgac
    14581 aaacgcgtgc tggtggtgga tggcgagccg gtaccgtact gcctggcgcg tattccgcag
    14641 gggggcgaaa cccgtggcaa tctggctgcc ggtggtcgcg gtgaacctcg tccgctgacg
    14701 gaaagtgact ggaaaatcgc ccgtcagatc gggccgacgc tgaaagaaaa agggctgatt
    14761 tttgttggtc tggatatcat cggcgaccgt ctgactgaaa ttaacgtcac cagcccaacc
    14821 tgtattcgtg agattgaagc agagtttccg gtgtcgatca ccggaatgtt aatggatgcc
    14881 atcgaagcac gtttacagca gcagtaaccc accttagcga gaaggatctc gttgagactc
    14941 tgagtgacag cgcccttctt tccacgcata ctgggcgctg ttgctttttt gaaccaggaa
    15001 acagaacctc tgacaatgaa tttacagcat cactttctta ttgccatgcc tgctctccag
    15061 gatccgattt tccgtcgttc cgtggtctac atttgcgaac ataataccaa tggtgcaatg
    15121 gggatcatcg tcaacaagcc gctggaaaat ctcaaaattg aagggattct ggaaaagctg
    15181 aagatcacgc cggagccgcg tgatgaatca atccgtctgg ataaaccggt tatgctcggc
    15241 ggtccgctgg ctgaagatcg cgggtttatt ttgcatactc cgccctccaa ttttgcttcc
    15301 agcattcgca tttcagacaa cacagtgatg accacttccc gcgatgtgct ggaaacgctc
    15361 ggcaccgata aacaaccgtc tgacgtattg gtggctctag gttatgcctc ctgggaaaaa
    15421 ggtcaactgg aacaagaaat tctcgataac gcgtggctaa cggccccggc ggatctgaat
    15481 attctgttca aaacgccgat tgccgaccgc tggcgcgagg cggcaaaact gattggtgtg
    15541 gatattctca ccatgcctgg tgtggcaggg cacgcctgat gagtggaacc ttactcgcct
    15601 tcgacttcgg caccaaaagc attggcgtag cggtcggcca acgcattacc ggcactgctc
    15661 gccctttgcc tgcaattaaa gcacaggacg gtacgccgga ctggaacctc atcgaacgtt
    15721 tactgaaaga gtggcagccg gacgaaatca tcgtcggttt gccgctgaat atggacggca
    15781 ccgagcaacc attgactgcc agagcgcgta aatttgccaa ccgtattcat ggtcgtttcg
    15841 gtgttgaagt aaagctccat gacgaacgtc ttagcaccgt ggaagcccgt tccggtctgt
    15901 ttgaacaggg cggctatcgg gcgctcaaca aaggcaaagt cgactctgcc tctgcggtta
    15961 ttattctcga aagctatttc gagcagggat attaaggcga tttaaaagcc tggcggatag
    16021 tcgttaccgc catcttgccg gatgcggcgt gaacgcctat ccggcctaca tgtggcagcg
    16081 attgtaggtc tgataagacg cgcaagcgtc tcatcagacg tttattgcct ttgtaggccc
    16141 gataagcttg cacatcgggc atggcaacgt cacaaacgcc cttcccccac tcgctgctga
    16201 aaactctgct gaaacgttat catccccacc tgctgcccgg tttgaataac atgcggtaac
    16261 tggtgggttt tcccttcgcg aatcaaattc cccaccgcgg gagtgttaat caataattca
    16321 aacagcgcca cgcgtccttc ctgtttatcc acttccagct tttgtgacaa cacggcccgt
    16381 aaactacctg ccagttgatt acgtacggga tctttttcct gcgccggaaa tgaatccacc
    16441 agtcgctcaa ctgcctgcgc ggcaccacgc gtatgtaatg ttgccagcac caaatgcccg
    16501 gtttctgccg ccgtcagtgc cagacggatt gtctcgctgt cacgcagctc tccgagcaaa
    16561 atcacatcag gatcttcccg caatgcggcc cgcaatcccg atgcgaacgt catacagtgc
    16621 aaaccaattt cccgcttctg gatcaaacat cgctggctgg catagatata ttcaacagga
    16681 tcttccagcg tcagaatatg cgcatcggca tgttgattga gatagccaac catcgccgcc
    16741 agcgtggtag atttgctgct ccccgtcgcc cccgtcacca gaatcaggcc attctcactc
    16801 ttgagtaatt ccggcaatac cgttggtgcg ccaagctgtt cgagctgcgg gcagtgcgaa
    16861 ggtaacagcc gtaacgccag cgaaatgcca tgccgttgcg cgaatgcgct gccacgcaat
    16921 cgctggtttt ccgccagcga cacggcaaaa tccagctggc cattttccag caatattgcc
    16981 cgctgatcgt catccagcca ctcccgcagt agctcttcga cgtccggcgc atcaaacggc
    17041 gcagcttcca ttagcccgcg aatgcgccat cgtgcgggcc aggcgctgca caggtgtaga
    17101 tccgagacgt tatgctttac actaagggcc acaatttctt ccatattcat actaagatcc
    17161 tcggaaaatg aacgatattg cgcataacct ggcacaggtc cgggacaaaa tctcagcggc
    17221 tgcaacgcgt tgcggccgtt ctccagaaga aattacgctg cttgcagtca gtaaaacaaa
    17281 acctgcgagc gccatcgcag aagccattga tgccgggcag cgtcaatttg gtgaaaacta
    17341 cgttcaggaa ggggtagata aaattcgcca ctttcaggaa ctgggcgtaa caggattaga
    17401 atggcatttt attggcccgt tgcagtctaa taaaagccgc ctggtggcag agcatttcga
    17461 ctggtgtcat accatcgacc gtttgcgcat tgctacccgc ctcaacgatc agcgcccggc
    17521 agaacttccc cctcttaacg tactgattca aattaatatt agtgatgaaa acagtaagtc
    17581 cgggattcaa ctggcagaac tggacgagct ggcagcggcg gtcgctgaac taccgcgttt
    17641 acgtctgcgc gggctgatgg caatccctgc gcctgagtca gaatatgtaa ggcagtttga
    17701 agttgcacgc caaatggctg tagcatttgc cggactgaaa acgcgctacc cgcatatcga
    17761 cacgctctct ctgggaatgt cggacgatat ggaagccgcc attgcggcag gtagcacgat
    17821 ggttcgtatc ggcactgcaa tttttggtgc gcgtgattac tctaaaaaat aaggaattaa
    17881 aggaacgcca tgaatacgtt gactttcctg ctttcaacgg tcattgagct gtataccatg
    17941 gtgctgttat tacgcatctg gatgcagtgg gctcattgtg atttttacaa ccccttctca
    18001 cagtttgtag tgaaggtaac gcagccaatt atcgggccac tgcgccgcgt tattccggca
    18061 atgggcccca ttgacagcgc ctcgctgctg gttgcctata ttctcagttt tatcaaagcc
    18121 atcgtgctgt ttaaagtggt gaccttcctg ccaatcatct ggattgctgg tttactgatt
    18181 ctgctgaaaa ccatcggcct gctgattttc tgggtcctgc tggtgatggc gattatgagc
    18241 tgggtaagcc agggtcgtag tccgattgaa tacgtgctga ttcagctggc cgatccgctg
    18301 ctgcgcccga ttcgccgcct gctaccggca atgggtggga ttgatttctc gccgatgatc
    18361 ctcgttctgc tgctgtatgt catcaatatg ggtgtcgcag aagtattaca ggcaaccgga
    18421 aacatgctgc tgccggggct gtggatggcg ttatgagtgc cgtaacagtt aatgatgacg
    18481 gtctggtttt acggctctat attcagccga aagccagccg tgattctatt gtcggtttac
    18541 atggcgacga agttaaagtc gccattaccg cgccgccggt tgacggccag ggcaacagtc
    18601 atctggtgaa gtttctcggt aagcaattcc gggttgccaa aagccaggtg gtgattgaaa
    18661 aaggcgaact tggccgccac aaacaaatta aaatcattaa tccgcaacaa atcccgccag
    18721 aaatcgcggc gttaattaat taggtatcct atgcaaaaag ttgtcctcgc aaccggcaat
    18781 gccggtaaag tgcgtgagct ggcgtcgctg cttagcgact tcggtcttga tatcgtggcc
    18841 caaacagacc tcggcgtaga ttccgctgaa gaaaccggcc tgacctttat cgaaaacgcg
    18901 attctgaaag cgcgctatgc ggcaaaagtg accggtttac cggcaattgc cgacgactct
    18961 ggtctggcgg tagatgcgct gggtggcgcg ccgggtattt actccgcgcg ttattccggt
    19021 gaagacgcga ccgatcaaaa gaatctgcaa aaactgttgg aaacactgaa agacgtaccg
    19081 gacgaccaac gtcaggcgcg tttccactgc gtgctggtat atctgcgtca cgcggaagat
    19141 ccgactccgc tggtatgcca cggcagctgg ccgggcgtga ttactcgcga accagcgggc
    19201 actggtggct ttggttatga tccaatcttc ttcgtacctt ccgaagggaa aaccgccgcc
    19261 gaactgaccc gcgaagaaaa gagcgccatt tcccaccgtg gtcaggcgtt gaaactgctg
    19321 ctggacgctt tacgtaatgg ttaaattacc gccgctgagt ctctacattc acatcccgtg
    19381 gtgcgtgcag aaatgcccgt actgcgattt caactctcac gcgttgaaag gagaagtgcc
    19441 gcacgacgat tatgttcagc atctgctttg cgatctggac aatgatgtgg cttacgctca
    19501 gggccgtgaa gtaaagacaa tctttattgg cggtggtacg ccgagcctgc tttccggccc
    19561 ggcgatgcaa acgctgctgg acggcgtgcg tgcgcgtttg ccgctggcag cggatgcaga
    19621 aattactatg gaagcgaacc ctggaacggt agaagccgat cgctttgtcg attatcagcg
    19681 tgctggtgtg aaccgcatct ctattggtgt gcagagtttt agcgaagaaa agctgaaacg
    19741 acttgggcgt attcatggcc cgcaagaagc gaaacgcgcg gcaaagctgg cgagcgggct
    19801 ggggctgcgt agctttaacc tcgatttgat gcatggacta ccggatcaat cactggaaga
    19861 ggcgcttggc gatctacgcc aggccattga actgaatccg ccgcatcttt cctggtatca
    19921 actgaccatc gaacccaata cgctgtttgg ttcgcgacca ccggtgctgc cggacgatga
    19981 cgcgctgtgg gatattttcg aacaggggca tcagttatta accgcagcgg gttatcagca
    20041 atatgaaact tccgcttacg ccaaacccgg ttatcagtgc cagcacaatc tcaactactg
    20101 gcgatttggt gactacatcg gtattggctg cggcgcgcac ggcaaagtca ccttcccgga
    20161 tgggcgcatt ctgcgtacca ctaaaacgcg tcatcctcgt ggttttatgc aaggaaggta
    20221 tctggaaagc cagcgtgatg tcgaagccgc agataagccg tttgagttct ttatgaatcg
    20281 cttccgtctg ctggaggccg cgccgcgcgt ggagtttatt gcgtataccg ggctttgcga
    20341 agatgtgatt cgcccacagt tagacgaggc gattgcccag ggttatctca ccgaatgtgc
    20401 ggattactgg cagataaccg agcacggtaa attattctta aactcacttc ttgaattatt
    20461 cctcgctgag taatcattaa tgcgcctgcg ggcgcattaa tcacttcttt tccccccgtt
    20521 aattaacttc ggcaaaatat atttcttact gaataaacat cgatgatatt tattagatat
    20581 atctagtgaa tatttactca tcatttattg attataaaca tgtacaacct ggcgcacaaa
    20641 tttaatattt aatttctgac tttatttcaa gttgtgataa acatagacaa gttgtcagac
    20701 aggttaacca atttgtcgca caaagcactg ataagcacaa ttagatatca accaatttcc
    20761 atccttcaac agggagtttt tttatgaaaa tgaggtcatt tacccgctca ttagtctgcg
    20821 catccttgct ggctctggtt agcactggcg taaacgccgc agaaaaagtg acgttaaagt
    20881 tagcacacaa cctggaaaga agtcatgttg tacatcagag ttttgaagag ctggcgaaag
    20941 aggtaaaaca gctgtcagaa ggtaatatgg tgatccgtat ttatcccagc agccagatgg
    21001 gtaatgcgcg agaaactatg gaattgttgc aaaatggcgc actggatatg accaaaggtt
    21061 ccgccagtga tctggagtct tttgataata tttatgctat ctataatttg ccgttcttat
    21121 ttaaagatca ggctcacttc aacaaagttg tttttggtga agttggtaaa gaaattatgg
    21181 actcaacaaa agacaaaggc ttttttgctt tatcagctta tgtagcgggt acacgcagtt
    21241 tctatgcgaa aaaaccgatt actaagccag aagatcttaa agggctgaaa attcgtgttc
    21301 agccaagtcc gaccaccatc aaaatgattg aactgatggg tggttcaccc actccaattt
    21361 catttggcga agtttatact gcgatgcagc aaggtgtagt agatggcgca gaaaataacg
    21421 tgccttcctg ggtgcagact cgacatattg aaattgccaa agttttttct gaagatgaac
    21481 acgcctcaat tcctgacttt ttggttatct ccaccaaaac atggaataaa ttaaccccag
    21541 aacagaaaca aatcctcgaa acagcagcga agaaatccga agcttatcaa caaaaattgt
    21601 gggaaaaaat tgacgctgac acacgtgctc aggcgaaagc tatggggggt gaaattgtaa
    21661 aagtggataa agcaccattc cgtgccgcag ttcagcctct gtttgacgac ttcaaaaaag
    21721 atccaaaaca agctgctttg ttagagaagt tcgacaacgc tgctcaataa tattccgggc
    21781 cagtctctgg ccctgatttt ccaagatgct tacaatgata tttaaccgcc tgaaacttgc
    21841 ggtagaccgc gtcattgccg cgttttctgt cgccgtcatg cttgctctgg ttgtatgcgt
    21901 agtctggcaa gtatttagcc gctacgtact taaccagcca agtacattaa ccgatgagct
    21961 ggcacgattt ttgatgatct gggtagggtt attaggtg
//
LOCUS       DAKISA010000074        21971 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.074, whole genome shotgun sequence.
ACCESSION   DAKISA010000074 DAKISA010000000
VERSION     DAKISA010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21971
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.074"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(121..330)
                     /locus_tag="OVW45_002824"
                     /pseudo
     CDS             complement(121..330)
                     /locus_tag="OVW45_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
     gene            complement(532..1176)
                     /gene="cheZ"
                     /locus_tag="OVW45_002825"
     CDS             complement(532..1176)
                     /gene="cheZ"
                     /locus_tag="OVW45_002825"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCV5527228.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGLVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKVGVVASQDQVDDLLDSLGF"
     gene            complement(1187..1576)
                     /gene="cheY"
                     /locus_tag="OVW45_002826"
     CDS             complement(1187..1576)
                     /gene="cheY"
                     /locus_tag="OVW45_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCV5527229.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1591..2640)
                     /gene="cheB"
                     /locus_tag="OVW45_002827"
     CDS             complement(1591..2640)
                     /gene="cheB"
                     /locus_tag="OVW45_002827"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCV5527230.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(2643..3503)
                     /gene="cheR"
                     /locus_tag="OVW45_002828"
     CDS             complement(2643..3503)
                     /gene="cheR"
                     /locus_tag="OVW45_002828"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCV5527231.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3522..5123)
                     /gene="tap"
                     /locus_tag="OVW45_002829"
     CDS             complement(3522..5123)
                     /gene="tap"
                     /locus_tag="OVW45_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HCV5527232.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5169..6830)
                     /gene="tar"
                     /locus_tag="OVW45_002830"
     CDS             complement(5169..6830)
                     /gene="tar"
                     /locus_tag="OVW45_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HCV5527233.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVARVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(6975..7478)
                     /gene="cheW"
                     /locus_tag="OVW45_002831"
     CDS             complement(6975..7478)
                     /gene="cheW"
                     /locus_tag="OVW45_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCV5527234.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7499..9463)
                     /gene="cheA"
                     /locus_tag="OVW45_002832"
     CDS             complement(7499..9463)
                     /gene="cheA"
                     /locus_tag="OVW45_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HCV5527235.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSMAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVGDEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9468..10394)
                     /gene="motB"
                     /locus_tag="OVW45_002833"
     CDS             complement(9468..10394)
                     /gene="motB"
                     /locus_tag="OVW45_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCV5527236.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEDLKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10391..11278)
                     /gene="motA"
                     /locus_tag="OVW45_002834"
     CDS             complement(10391..11278)
                     /gene="motA"
                     /locus_tag="OVW45_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCV5527237.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11404..11982)
                     /gene="flhC"
                     /locus_tag="OVW45_002835"
     CDS             complement(11404..11982)
                     /gene="flhC"
                     /locus_tag="OVW45_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCV5527238.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11985..12335)
                     /gene="flhD"
                     /locus_tag="OVW45_002836"
     CDS             complement(11985..12335)
                     /gene="flhD"
                     /locus_tag="OVW45_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCV5527239.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
     gene            13115..13543
                     /gene="uspC"
                     /locus_tag="OVW45_002837"
     CDS             13115..13543
                     /gene="uspC"
                     /locus_tag="OVW45_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HCV5527240.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(13550..14974)
                     /gene="otsA"
                     /locus_tag="OVW45_002838"
     CDS             complement(13550..14974)
                     /gene="otsA"
                     /locus_tag="OVW45_002838"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HCV5527241.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(14949..15749)
                     /gene="otsB"
                     /locus_tag="OVW45_002839"
     CDS             complement(14949..15749)
                     /gene="otsB"
                     /locus_tag="OVW45_002839"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCV5527242.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(15916..16902)
                     /gene="araH"
                     /locus_tag="OVW45_002840"
     CDS             complement(15916..16902)
                     /gene="araH"
                     /locus_tag="OVW45_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCV5527243.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(16917..18431)
                     /gene="araG"
                     /locus_tag="OVW45_002841"
     CDS             complement(16917..18431)
                     /gene="araG"
                     /locus_tag="OVW45_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCV5527244.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRSIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(18501..19490)
                     /gene="araF"
                     /locus_tag="OVW45_002842"
     CDS             complement(18501..19490)
                     /gene="araF"
                     /locus_tag="OVW45_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HCV5527245.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDALSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            20287..20790
                     /locus_tag="OVW45_002843"
     CDS             20287..20790
                     /locus_tag="OVW45_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCV5527246.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(20869..21120)
                     /locus_tag="OVW45_002844"
     CDS             complement(20869..21120)
                     /locus_tag="OVW45_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCV5527247.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(21235..21321)
                     /gene="azuC"
                     /locus_tag="OVW45_002845"
     CDS             complement(21235..21321)
                     /gene="azuC"
                     /locus_tag="OVW45_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCV5527248.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            21584..21907
                     /locus_tag="OVW45_002846"
     CDS             21584..21907
                     /locus_tag="OVW45_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCV5527249.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT         5368 a         5841 c         5178 g         5584 t
ORIGIN      
        1 gagtcggcga attacatagg ggctcgcagc gttacggctg caataaagcc ggatataaag
       61 ctgcaaccta cccgtcatgt caaaacaatg gatgccttgc aaacgggatg cgttcatata
      121 aatactgtga tcaaaatgca gcccagcgga gagcatgccc gacaatcgac gggccagcga
      181 cacaccgcca aaccagataa cacacacgcc cactaacaaa atcagcaacg aggtcagttc
      241 acgggaacgc gggatttgcc cctcttcccg cgctttttct agtcggtgag gtgtgggggc
      301 ttctgttttg tcgtcgctct cgtcagacac gtcgccaatc ctggaaagag tattaagctt
      361 aaatgatgcc agagcgcaaa gcgttcaatg gtttgagtaa ggggcaaaac aggcgggatt
      421 tagggctttt gctgccacac atcaagcata gtgtgcgttt gtcggatgcg gcgtcatcgc
      481 cttatcagac cgcctgatat gacgtggtca cgccacatca ggcaatacaa atcaaaatcc
      541 aagactatcc aacaaatcgt ccacctgatc ctgactggct accacgccga ctttgctggt
      601 atcgacctga ggtccattaa gcaaactctg gttttcacgt tttggacgcg actcctgttc
      661 cgggatgttt tccaacagca ccatcagcaa ctggcgttcg atctcctgaa tgacatccat
      721 catccgctta atgaccagcc cggtgagatc ctgaaaatcc tgcgccatca tgatttccag
      781 cagttgcgcg ttagtaaagc tggtatgcgc gggtacatct gccagaaatt gtcgtgtatc
      841 tgttaccagt tcacgggcgt cggcaaggtc aatcggatcg gcaaaccagt catcccaacg
      901 ttgggttaac gcttttgctg atttctccat ttgatcctga tgcggttgtg acgcctcaac
      961 actgttcagc gcccgctccg cagcctgggc ggtcatctgc acaacatagt acaaacgatc
     1021 gcgcgcatcg gggatggctt ccgccgcttc ggcaatggcc tgatccagcc ccagttcccg
     1081 caaactgtcg cgcagcatac gcgtcaggct gccgatgcgc gcaatgatat cgccagctga
     1141 atgctcgtca gcaggtttga ttgatggttg catcatagtc gcatcctcac atgcccagtt
     1201 tctcaaagat tttgttgagt ttttcctcca gcgtcgcggc ggtaaatggc ttcaccacat
     1261 agccactggc ccccgcttgc gccgcagcaa tgatgttctc tttcttcgct tctgcagtca
     1321 ccattaacac tggcaatgcc gacatcgcgc catccgcacg aattgttttc agcaattcca
     1381 ggccatccat attgggcatg ttccagtcgg agataacaaa tccataaccg cctgcctgca
     1441 acttattgag agcgtcgacg ccatcttccg cttcctcaac attattgaat cccagctctt
     1501 tcagcaggtt acgcactatg cgtcgcatgg tggaaaagtc atccacaacc aaaaatttaa
     1561 gttctttatc cgccatttca cactcctgat ttaaatacgt atcgcctgtc cggcactaat
     1621 ttttgccaac atttgctggc ttacctggct aagatcgacc acttcgcaga caccacccat
     1681 attgatggcc tcgcgcggca tgccgaacac cacgcaactt gcttcgtttt gcgcaagggt
     1741 ccatgccccc gcctgacgca tcgccaacat tcccgccgcg ccgtcgttgc ccataccggt
     1801 caggatcacc ccaaccgcat tacgccccgc ctgtttggcg acagaatgga acaacacatc
     1861 taccgaaggc cgatgacggt taaccgccgg gccgtcgtga attttgattt gataatttgc
     1921 gccactacgc gccagttcca tatgccgatc gcccggcgca atataggcat gccccggcaa
     1981 gacacgttct ccgtcttcgg cttctttaac cccgatctgg caaagcttat taagtctgtc
     2041 ggcaaaagag cgggtgaaac cgggcggcat atgttgggta attaacaatg ccgggctgga
     2101 aagcggcaac ggttgcagta cgtgacgaat tgcctcagtt ccacccgttg aagcaccaat
     2161 cgcaatcagt ttttcagaac tcaacaacgg acccgccttc agcgttgtcg gtgctgacaa
     2221 tggcttatgt gctgcaaggc tcgcctttgc tgccgtacgc accttttcag caatcatttc
     2281 gctatacgcc agcattcctt cgcgaatacc cagttgcggt ttggtgacaa aatctattgc
     2341 ccccagctcc agcgcgcgca gcgtgacttc tgatcctttg ccggtcaagg aagaaaccat
     2401 cacaacgggc attggacgca aacgcattaa tttttcgagg aaatccagtc cgtccatccg
     2461 cggcatttca acatccagcg tcagcacatc gggattgaat ttcttaatca agtcacgcgc
     2521 gaccagcgga tcaggcgcgg tcgccaccat ttccatgtcg ctatggctgt tgatgatttc
     2581 tgtcatgatc tggcgcatca gtgccgaatc atcgacagat aacaccctga ttttgctcat
     2641 cgttaatcct tacttagcgc atacaccgtc tgaccacgca gcgtgaagcg gcgctcaagg
     2701 tggctaaagt tttcagagtg acccgcaaac agcaatccgt cgggtttaag gagcggaaca
     2761 aagcggcgca aaatctcctg ctgggtattt tgatcgaagt agatcatgac gttacgacag
     2821 aagatcgcat caaacggccc cggcacggtg tactgtttcg ccagtagatt cagcggggca
     2881 aaatcaacat agttcgccag ctcctgacgc acgcgtacca gcccttcatg cggtcccgtc
     2941 cctcgcatga aataccgttg cagttgctgc ggcgtcaggt ttttcaactc ttcatggcga
     3001 taaataccgc ttctggcttt ttccagcact tcggtgtcga tatcactggc aaacactttc
     3061 cagcgtccgg gcgcggtgcc caatgtgtca gccagcgtca tcgcaatgct gtacggctct
     3121 tcgccggtcg aagccgccgc gctccatacg cgatactcgc cagaacggcg acgtgcgtga
     3181 tccgcgagca gagggaaatg gtgtgcctca cggaaaaatg ccgtcagatt cgtggtcagc
     3241 gaattgataa acgcctgcca ctcaccgctg tgctgattag attccagcaa gttcagataa
     3301 tgaccgaaat ccgtcagtcc cagcgaacgc aaacgacgaa ccagtcggtt gtaaaccatg
     3361 tcgcgtttat ggtcagccag aacgatcccg gctcgttgat agatcaattg acttatccgc
     3421 cgaaaatgcg cgtcggaaag cgccaggcgc tcggtcatct gtaacaataa agacgtttgc
     3481 ccacagggca gagatgaagt catagcgcct tctcaatcac ttcaggatac cactggcgca
     3541 atttgtaact gcgccgactc atgtcgtgct acttcatgtt cttcaagggt aaataccgcc
     3601 acacgcgacg aaagatggtc ggcctgattc gccagttgtt ccgttgccac cgccgcttct
     3661 tctaccagcg aggcgttctg ctgagtcacc tgatccatct ggctgacagc ctgtgcaacc
     3721 tgctcaatcc cccgctgttg ttcttccgac gcagaggcaa tttctcccat aatgtcgttc
     3781 acgcgggtca ccgaactgac aatatcgatc atggtcgcgg cggcgttatt caccagtttc
     3841 gaaccctgct ggacacgatt gactgactct tcgatcagcc ctttgatctc ttttgctgcc
     3901 tgcgcgctac ggctggcaag attgcgcact tcacctgcca ctaccgcaaa accacgcccc
     3961 tgctctccgg cgcgagccgc ttccactgcc gcattcaggg ccagaatatt ggtctggaaa
     4021 gcaattccgt cgataacgct gataatgtcg ccaatttttt gcgagctggt ggcgatctcc
     4081 tgcatggtgt gagtcatggt actgacctga acaccgcccg cctgcgccgt tgtcgcggca
     4141 ttttttgcca gttccgacgc ctgtcgtgcg ttatcggcgt tttgccctac cgtggcggtt
     4201 aattgctcca tactggcggc cgtttgtgcc agcgatgccg cctgctgttc ggtacgactt
     4261 gagagatcgt tattgcctgc gacaatctcc gcgataccaa tgtgcatctc ctggcttccc
     4321 ttacgcacat cacttaccgt cccacgcaaa gcctgctgca tggtcttcag actggcaaaa
     4381 atggcggtga tctcattacg accatatacc gcaatcggac gcgccagatt acccgcagca
     4441 atgctgtcaa aatggctacc gataatggcc agtggttgaa caatcatctt gcgcgtccac
     4501 cacagcgcac tgctgatgta gatcgccgca acaataatca tgctgataaa caccagtgcc
     4561 gaaatctgat agttacgctg gctttgcgca ctggcggctt ccagcacatg gttgatctcc
     4621 agctgccagg cctcaaagtt aacgtcaaac gcattctgtg atccctgcac cggcgcagtg
     4681 aggaaatccg aaagttgatt actttcaagc caggtcgcct gatgttcgag atcgttgtgc
     4741 cagcgggcaa aacttttctc cgtctctttt tgcaatgccc tgacgtgctc gttgcccgca
     4801 gtcatcgcca taaaactttt aaacagcgtg gtggattgcg tcagactggc gcgcgccgtc
     4861 gtcatcaacg ttttaatgtc atccgccgga tagctaagtg ccgtcagagt gcccgctttg
     4921 ttcagcgcgg tactggcctg taacattacc gcccgagttt gcgctaatgc cgcacgttgc
     4981 tgattgctct gctcgacctg attcagtcgt tgcaaatcgt cgcgaaatgc ccaaaaagac
     5041 atgccgttac tgccaatctg caagatcccg cagagaatca aaattaaaaa cagcgtggtc
     5101 gaaattcgaa tacgattaaa catcaacgct ccccatcagg cggcaatgac cgcgttagta
     5161 aatactcgtc aaaatgtttc ccagtttgga tcttgttcag caattcgcag tcgtggctgt
     5221 gccggtggtt gctcactggc aggacgggat ggtgtttgcg gtttattggt gagtgggctg
     5281 gctgccagac ggaacgcgga aaccgcttgc gttaaacgac tcgcctgttc ttccagcgca
     5341 gcggcggcgg cagctgattc ctgcaccagc gatgcgttct gttgcgtgac gcgatccatt
     5401 tccgaaaccg ccaatgcgac ttgatcgatg ccacggctct gttcatccga tgccgatgca
     5461 atctcgccca taatgtcagt cacgcgagtg acagcattga cgatattgtt cattgtttcc
     5521 ccggcgcttt cgaccagcac cgaaccggta tcaacgcgtg cgacggagtc ttcaatgagg
     5581 gctttgatct cttttgccgc ctgggcgctg cgactggcaa gattacgcac ttcacccgcc
     5641 accacggcaa aaccacggcc ctgttcaccc gcacgcgcgg cttcaaccgc ggcattcagc
     5701 gcgaggatat tagtctggaa ggcaataccg tcgataacgc tgataatgtc ggcaattttc
     5761 ttcgaactat cggcgatctc atgcatcgtt ttcactacgc catccaccac tttgccgccg
     5821 tgctgggcgg tgtcggaggc actttgcgcc agttgcgagg cctggcgggc gttatcggcg
     5881 ttttgcttca ctgtcgcggt gagctgctcc atgctggcgg cagtttcttc cagcgcggat
     5941 gcctgctgtt cagtacggga ggaaagatcg gtgttgcccg ccgcaatttc acgggtaccg
     6001 gcatagatgg catctgaacc ttcgcggaca tgagtgacgg tgtcagtcaa agagcgttgc
     6061 atatgtgaaa cgctctgcgc caggtcgccc atttcactgc gcccgtcaat ggtcagggta
     6121 ttcgctaggt taccaccggc gatttcgcga atgtgagcaa taatttttgc cagcggagta
     6181 agcaacatac ggcgaatgcc gtaccacgcc accagcagaa tcaataccac caccagcgcg
     6241 ataaccgcca gttgccactg ggcaaatcgg taatcatctg cgttgtcagt gacgatatcg
     6301 cgatacagtt tttcactgct gagggcgtac tgagcaaacg cttcgcccat tgcattttgc
     6361 attccctggg ttggctgagc gaaataagct ccagtattgc cataatctag ataatcaatc
     6421 agttcagtta acgctgtgta atagttttta tatttttcat caatattacg actggtagcg
     6481 accatttcag gtaacggtgc catgcttttg aattttttat aatgcgtcgc tgcctgcgcc
     6541 aatgttttcc tggcgctatc gagcaattca actttggcgt tactttgttg attggaggaa
     6601 tccatcatca tccgcaccgc tgaacgactc aggttaatgc gcgtttgcag cattaaatcc
     6661 caggttgacg tcagctcgcc ctgctgttcc cgcaattgat tggaaaccac aaagctcttc
     6721 tggctatggt gaagggaaga aaaaaacaga ctgccggaaa taagctgtaa cagtgcgaat
     6781 acccccagca ccattaccaa cagcgtgact acgcggatac ggttaatcat aaggcacctt
     6841 cctgaaaaca agttgatctc gttatcggca agaagggggg aaactttatt gctgatgcca
     6901 cccgccgcga aattgaaata aaaaacccga tgcgcagatc atcgggttca tttcaattga
     6961 ggaaatcggg agaattacgc cacttctgac gccgcgctat ctaacagcgc catctcttcg
     7021 ctgttcagca gtttttcgat gttcaccaga atcaacatac ggtcgcccag tgcgcccagt
     7081 ccagtgagat attctgttga aagcgtcacg gcaaactctg gtgccggacg aatttgctcc
     7141 gccgtcaatg aaagcacgtc tgaaacgccg tcaaccacga tgccgaccac ccgctgtccg
     7201 agattcagga cgataactac cgtgttgtcg ttatagtcca catccacctg gctgaactta
     7261 attcgtaagt caacaatcgg cacaataacg ccgcgcagat tcgtgacgcc tttgataaac
     7321 gctggcgtgt tcgcaatccg tgttacctga tcgtagccac ggatctcctg cactttcagg
     7381 atatcaatac cgtactcttc atcaccaagg gtaaatacca gaaattcctg gcctgacggc
     7441 tcgctggcca gctttgttac attcgtcata ccggtcatat tgttaccttt ttactcattc
     7501 aggcggcggt gttcgccata cgttgttcgc ggtttatcgc ctgcaaggcg gagacatcaa
     7561 caatcagtgc cacgctgccg tcgccaagaa tggtcgcagc agaaatgccg gggactttgc
     7621 gatagttact ttcaaggttt ttaaccacaa cctggtgttg accaattaat tgatccacca
     7681 gcaaggcgta gcggcgaccg ccactttgta agatcacaac aattccctgg gtggcttcgg
     7741 ttttcgcgcc cgcgacgttg aacactttcc acagttcgac gatgggcaga tattcacccc
     7801 gcacttccag cacccgctcg ccgccggcca gtggatggag atcggcttca cggggttgca
     7861 gtgattccat aacagcattc agcggcagaa tgaaaacttc atccccaacg cgtacggaca
     7921 tgccgtcgag gatggccagc gtcagcggca gtaaaatgcg gatcgtagtg ccagtcccct
     7981 gcttcgactg gatttcgaca tgaccgccca tctcctggat attacgttta acgacgtcca
     8041 tgccgacgcc gcgcccggag acgtcggtga cctgctctgc catggagaag cctggtgcaa
     8101 atatcagcat cgcgacttcg tcgtcgctca tgttttcgct gacagtcaaa ccttgcgagg
     8161 ccgcttttgc cagaattcgc tcacggttta gccccgcccc atcgtcggtc acttcaatgc
     8221 aaatgttgcc gccctgatgt tcggcagaca gaattaaatt tccgacgctg tttttacctg
     8281 cggcgagccg tttttctggc agttcaatac cgtgatcgag gctattgcgt accaggtggg
     8341 tcagcgggtc gataatgcgt tctatcaggc ttttgtcgag ttcagtagaa ctgcccacca
     8401 gcgtcagttc tacctgcttg ccgagttttc ccgccagatc acgcaccagc cggggatagc
     8461 gactaaaaac atattccatc ggcatcatgc gaatcgacat cactgattcc tgcaaatcac
     8521 gggcgttacg ttgtaactgc cccatgctgg ttatcaaatc accatgatta accgggtcca
     8581 gttcgctgga acgctgggca agcatggact gggtgataac cagctcgccg acgaggttaa
     8641 ttaattgatc aaccttttct accgctacac ggatgctggt ggattcattg ctgcgcgtcg
     8701 ttttttcccg ctccacgcgg ccggttggcg cttgttcggc tgccagttta agcactggtg
     8761 gggtggatat ttttggcgag acttctactg tttcaaaggt aatctgatcg gcttcaatca
     8821 caaaacagag taccgctgtg atgtcatctt cggcgatgtc gcccggtaat attgccgaga
     8881 gcgaatccgc ccctttcacc acgtcagtta acgttgtcag atgtcccagt tcttcttcca
     8941 gcaggtcgac ttccccggcc ttcaggcgcg aaaggataat tcgtcgcggc gactgactgc
     9001 gactctgctc atcttgcggt tcacttttgg caaccacact taatcgggtc actgcggatg
     9061 gcgtttcgcc tttcgcttct aatgccagtt gacgcaaggc ctggcagata taatcgaagc
     9121 tggcggcatc cggctcttgc gactgtttat aagcgtcgag ctgttcttgc atgatgtcct
     9181 tcgtttccaa aaacagattg ataatgtcgg tgttgagttg catctcacct cgtctggctt
     9241 catcgagcag gttttccatc agatgcgtgg tttcctgcaa aacgctgaag ccaaaagttc
     9301 ctgcccctcc tttgatcgag tgggcagccc gaaagatggc attcaattgt tcggcatctg
     9361 gcgcttccgg ctgcaaaacc agcagatgct gctccatgtc agccaacagt tcgtccgctt
     9421 catcaaaaaa tgtctgataa aaatcgctta tatccatgct cacgctgtca cctcggttcg
     9481 gctgatggca ttgtgggaac actgacctgt ggtgcaacct caggtttttc cagggcgctt
     9541 actggctcat tctggctttc ggcgttttca tgcaaaatgg cctgttcggc ttgtttgttc
     9601 agtaccagca ggctgatgcg acggttgacg gcatcatcag gtccgcgatc gcttaagcgc
     9661 atcgttgccg ccatgccgac gacacgtaac actttgccgc tatccaaccc tccgaccatc
     9721 agttcgcggc gggatgcatt ggcccgatcg gcagaaagct cccagttgct atatcctttc
     9781 tcaccgctgg cgtaggggaa atcatcggta tgacctgaaa ggctaatacg gttgggaata
     9841 ccgttcagta caggcgcaat ggcacgcaga atgtcgcgca tatagggttc gacatcggca
     9901 ctgccggttc taaacatcgg gcgattctgg ctatcgatga tctgaatacg tagaccttcc
     9961 tggaccagat cgattttgag atggggacgt aacgcccgca gtttcggatc ggactctatc
    10021 aactggtcga gatcaccccg caatttccgc aatcgacttt gctccatgcg ttttttcaga
    10081 tcttcgatgt tcggctgctt attcacttcc ccctggcttt gggtgtaatc atcaccaccg
    10141 ccgggaattg ggctttcact attagaaatg cgatcgccgc ccgtaaccgc agtcgccagt
    10201 ggagtccgga agtactccgc aatctgaatc agctcttttg ggctggagat ggagatcagc
    10261 cacatcacca gaaaaaaggc catcatcgca gtcataaagt cggcataagc aatcttccac
    10321 gatccatgtg ctgccccgtg gcttttggct ttgcgtcgtt tgacgacaat aatcggatgc
    10381 gcttgattct tcatgcttcc tcggttgtcg tctgttgttg cggatttttc accgcacgca
    10441 catgctcttc cagttcaata aacgacggac gttcgctgga atagagcgtt ttgcgaccaa
    10501 actcaacggc gataggcggt gcgtaaccgt tcagattaga aagcagagtg actttgacgc
    10561 actgcatcat tttgctggtt tcggcgcttt tctgacgtaa aacactcgct aatggggaaa
    10621 taaatccgta agccaataaa atgccgagga aagtccccac catcgcatgt gcgataagcg
    10681 cacccagctc ggcggcagga cgatcggctg aacctaacgc gtgaacgccc cccattacag
    10741 ccgcgacaat accaaacgcc ggaagtgagt ccccgaccag cgccagactg ttcgccggga
    10801 cttctgcctc gctttcgtgc gtctcaatct cttcatccat cagggcttcg atttcgaagg
    10861 tgttcatatg accgctgata atcagacgca gataatcgac gataaaatca agcatgacgc
    10921 tatccgcgag gatgcgtggg tagctggcga agatctcgct ctcacgggga ttttcaatat
    10981 cacgttccag cgaaaacatc cccatctgcc gcgatttcgc catcaaccga taaagcagag
    11041 ccagcagatc catatacatt gctttggtgt atttggagcg acgaaacagc aacggcagcg
    11101 ccttcagcgt gcctttaatc gctttgccat tattgccgac gataaacgac ccaatccctg
    11161 caccggcaat aatcaccagt tcagcgggtt gatagagtgc tccaaggctt ccaccggtca
    11221 tcaaataacc gccgaaaact gtaccgagaa caaccaggta acctaataag ataagcacga
    11281 catcatcctt ccactgttga ccatgacagg atgttcagtc gtcaggcgtt aacgcgcgat
    11341 tggggcaaaa aaagcagcgg tacgtcgtta ccgctgctgg aatgttgcgc ctcaccgtat
    11401 cagttaaaca gcctgtactc tctgttcatc cagcagttgt gggataatat cggcaggatt
    11461 ctgggaaagt ttacgtcttt ttactgcccg ggatggcggt tgacataagc tgcaggcaaa
    11521 gctgccaaca ggctggtgag cgtgggtaat aaaattgccg ccgcagcagt tgcagctgga
    11581 aagttgcagt aatccacttt caacaaaccg caccaatgtc caggcacggg ttaatgccag
    11641 cagtggtcct tcttctgctt gtgggcactg ttcaaggtat aaacggtagg ctttgatcac
    11701 cgcatcgacg ccattacaca aaccggtttt cagtaaaaac tgccatgcat tacagaacat
    11761 cgaagcatga acgttttgtt cccaggtcat aaaccagtcg gttgagaatg gcagcatgcc
    11821 tttcggcggt gggcttccgc gcagttcttt ataaagtttt atcaggcgtc cgcgacttaa
    11881 ctgtgtttcg ctttccagca tctgcaaacg agcgcccagg gtaatcaatt ccattgccag
    11941 ctgaatatcc cgcgcttcct gaacaatgct tttttcactc atgatcaggc ccttttcttg
    12001 cgcagcgctt cttcaggctg attaacatca ttcagcaagc gtgttgagag catgatgccg
    12061 gtatgaattt gctggagatc gtcaacgcgg gaatcttgcg tcaactgagt aatcgtctgg
    12121 tggctgtcaa aacggaagtg acaaaccagt tgattggttt ctgccagctt aaccatttgc
    12181 ggaagagtca gtgccgctaa cgttgtcgcc atttcttcat ttatgccgag acgaaacata
    12241 gcggacgctt tgtcctgaac aatcaaacgc tgtgcaagta gtaaatatga caagttgatg
    12301 tcataaatgt gtttcagcaa ctcggaggta tgcattattc ccacccagaa taaccaactt
    12361 tatttttatg cggtttcacc gcaccccgtg atgtcgccgg gaagccccgg taaaaaataa
    12421 ttagcattag aatagttgcg ataagctgca ataagcagaa ccaccttttt ggtttaatat
    12481 gtccttacaa atagaaatgg gtctttacac ttatctaaga tttttcctaa atcgacgcaa
    12541 ctgtactcgt cactacacgc acatacaacg gaggggggct gcgattttca ataatgtgtg
    12601 atgcagatca cacaaaacac tcaattactt aacataaatg tataagtcat acttttgttt
    12661 tgggtgtatt tcaatatgtt aaaaagtttt tcgctacgct agcaagcaaa aatgaaacag
    12721 gaataatcga aatgggatgt tgcgcacagt taaaataaat caccgtaaat aatcatctgc
    12781 tataaataat cactttcatg caataccaga taagctattt ttaaacagac acttaccgca
    12841 caacaaacta attaaataca aagaaaggca acaatcaact taatgttaat gaaatgtttt
    12901 acatatatta accacttgtt taatgcggag aatcagtaca ctattttctt ataattacat
    12961 ttgaaattat atgatcaccg ggagtgattg attaattgtc tttactaata atcagactaa
    13021 tatttacctg tttgaccgag ttgggattgc gtcgtttctc cattaggagt aaagctttaa
    13081 tgtcacctga agttcacaga ataaggaaca ggttatgagc tatagcaata ttcttgtcgc
    13141 tgttgcggtt acaccggaaa gtcagcaact gctggcaaaa gcggtatcta tcgccaggcc
    13201 agtaaaggga cacatcagtt taattactct cgcttccgac ccggaaatgt acaatcaatt
    13261 agccgcgccg atgcttgaag atttacgcag cgtgatgcaa gaagaaacgc aaagctttct
    13321 tgataagtta attcaggatg cggggtatcc cgttgacaag acgtttattg cctacggtga
    13381 gttaagcgaa catattctgg aagtatgtca caaacatcat ttcgatttgg tgatttgcgg
    13441 taatcacaat cacagtttct tttcgcgagc atcctgctcg gcgaaaagag tgattgcctc
    13501 aagtgaggtc gatgtgctgt tagttccact tacgggagat taaccgctcc tacgcaagct
    13561 ttggaaaggt agcaacttta tcgcgctgct ggctttccgc gcttcgcgga actatctgct
    13621 ttaggtcgct aatgaagcac tcctgccagt ggttaatatc gtttttcacg ataacgtcca
    13681 gcatttctgc atgacgggaa atacgttccg ccagcgacat agtcaatgca cgatccagcg
    13741 cagctgcaac ttcgtcacga tcgtaggggt taacaattaa cgccgacgtt aactcgtttg
    13801 ccgctcccgc aaattgcgaa agaacaagaa cgcccgggtt ggctgggtcc tgagcagcaa
    13861 catactcttt tgctaccagg ttcatcccgt cacgcagtgg cgtcactaag cccacgtcag
    13921 agtagcggaa tattttcatc agtaatttac ggtcaaaatg ctgattcaaa taataaagcg
    13981 gcgtccagcc taattgcccg tatttaccat taattcgtcc agcttcattt tcgagctgat
    14041 gacgaatatc ctgataggct tgcacatcac cacgcgacgt tggtgcaatc tgggtataac
    14101 gaattttacc atgatgctgc ggatattttt ccagcaacgc ttcataggcg agaaaacgct
    14161 ctggcaaacc tttggaataa tccagccgtt cgacagaaaa gatattttgt acgtttttca
    14221 gttccgcttt aagttgcgcc agttttggcg gcagtggccc ggcagcctgt ttggctattt
    14281 ctttcggttc aatgccgatc gggtagactt ctgttcgaaa tgctttgccc caggctgtat
    14341 ggctttttgc gctacgtgtc gtgacgcggg tcaggttaga aagacaatcc aggaacgcca
    14401 gacgatcgtt ttctgtctgg aaacccagca aatcataatc acaaagctgt tcaagcaagg
    14461 tgtcatatgt cggcagcgcg ttgaagattt ccggtgtcgg gaaaggaata tgcagaaaga
    14521 aaccaatgcg attattcact ccccgtttgc gtaattcatg cgcaaatggc aacaggtgat
    14581 aatcgtggat ccagataatg tcatcgtctt gcaacagcgg cagtaattta tctgccagca
    14641 acgcatttac gcgtagatag ccgtcccagg caggacgctg aaattgcacc agatcgagcc
    14701 gataatgaaa agcgggccag agaacggcat tggagaattg gttgtagtat tcgtcaaggt
    14761 cctgttcgct gaggttaaaa gaggcccacg taatgttacc ttttttcacc ttttttagcg
    14821 gctgatcctc attccctgtt tcaccactcc agccaaacca cagtccgcct gcggctttca
    14881 gtgcccccag tatgccaacg gcaaggccac cggcactggc ggcgtgctcg tctggtggtg
    14941 caatccggtt agatactacg actaaacgac tcatagtcat cactcctgtt attttctctt
    15001 ttttgttgta atgcggtggt tatcatttca agccagctcc agacatccgg cacacccgcc
    15061 agtcgccatg atgcctgagt tgcacctgtg ccaattttta ctgacattcc gcccagtcgg
    15121 ttaacgactg cgaagccaga ttcatcggtt aaatcatcgc ccagaaatac gggcgttcgc
    15181 ccgataaagg gagcttcctg cataaaagct gcaattgcct cacctttact ggtacctctc
    15241 ggtttgatct cgacaacaca ctttccctgc tgtaacgcca tttgtggcca gatctgagta
    15301 atacgttgcg ctaatgtcat taatgcgtct tcatgctgcg gagcctgacg ataatgcagc
    15361 gcaaaagcca tccctttcgc ctccagctcc gcgccgggat actgagcgat gactgtatgc
    15421 agttgcacgc taatatcacg cgcaatcgca tccggcagat gaacgatatg tgttttacca
    15481 ttgatgtcac ggcgctccgc cccatgcacg cccgctaacg ggaagcgata aggttttgcc
    15541 agtgcgtcaa gctccaccat tgagcgccct gatatcaatg ccaatgcacc atcacttgcg
    15601 gttgccagta gctgtagtcc ttgcagaata ttgtcaggca cgacgacctg atcgggatgc
    15661 ggtttgattt ccgccagcgt tccatcaaga tcaaaaaacc aggcatattt cgcggatagt
    15721 tcaggggttt cggttaacgg ttctgtcacc cggctctcct ccttctcttt tcttattgtt
    15781 ttgcgcagaa cgcgctttac agacatgtaa gtatagacag tgtgacgggg gtcgccattt
    15841 gcaatgaaga acagccaacc gttgtggcgg ctggctgtgg tgggaaaaaa cgttaaattg
    15901 ttgtggaaaa aagcatcaga cagtgcgttt cgctttttgc ttgtaacggt cgaagatcac
    15961 cgctgccagc aggattaagc cgcgaaccac gtactgcgcg aaaggagaaa tattaagcag
    16021 gttcatggcg ttttccacgg tgcctaaaat taagataccc gccaccacat atgagatttt
    16081 tccgatgcca cctttcagag aaacgccacc taaaacgcag gcggagataa caataagctc
    16141 ataaccaatc gacgtcattg gctgcccact agtcatacgt gaagccagaa taattccggc
    16201 tatcgcagat accaggccag agagaacaaa gataataatt ttggtgcgaa caaccggtac
    16261 acccgccaga cgcgcagcct cttcgttccc gccaatcgcc agggtgttac gaccaaaggt
    16321 ggttttattc agcaacaaac caaagataat cagacacgcg acggtgagcc agattggcgc
    16381 aggcagaccg aaccagttag cgtaaccaag ggcaaagaag ctttcatctt cgataccgac
    16441 cgctttaccg tctgaaatga tgtacgccag acctcgaaca atctgcatcg ttgccagtgt
    16501 tgtgatcaga gcatttattt tcagtttggc gataacaaag ccattgacca ggccacagag
    16561 aatgccaagc agcaaccccg ctgccacgcc aatccacagg ctttcagtca ggttgataac
    16621 caccgccgtg gtgacacccg cacaggcaat tacggaggcg acagaaaggt caaagtcacc
    16681 ggaagcaagg cagaacaaca tgccacacgc caccatcccc gacatggaaa ttgccaggcc
    16741 caaccctttc atattaatga aggtggcaaa atttgggaca aaaatggcac aggcgataaa
    16801 gagcaccgca aacaccacca gcatgccgta ctgatcccag atacgcccga agctgaatga
    16861 cgacttaggt gcgccagaac ccgatgtaga aacagaagac atcatactct ccttactcag
    16921 gcaactgcct ggctgacttt aggcatcgca aggctcagtg cctgacgctc atctgcctgc
    16981 tcgtgtaaca attcaccggc gatttcacct tcccgcatca ccacaatccg gtcggcaacg
    17041 ccgaggactt caggtaagtc gctggaggca aacagcaccg ccacaccctg cgccgccagc
    17101 gcataaatta cgttatatat ttcgtgctta gcaccaacat caatgctgcg cgtaggttca
    17161 tccagcaaaa tgaccttcat ctcttccgat aaccagcggc ccagaatggc tttttgctga
    17221 tttccgcctg agagattcat gatcagttgc tcagcgcccg gcgttttgat gttgagcgaa
    17281 cgaatgtgct gatcggcatt gttttcttcc caaccgttgt tgattacaca accgccgagc
    17341 acatgtttgc gtctggcact gatgttgata ttgtcgcgaa cggagtgcac gggaataatg
    17401 ccttccgctt tgcgatcttc cgggcagagc atcatgcctg cggcaatggc gtggctcggt
    17461 ttacgaatat cgatcggctg ttggtcgata taaacctgac cggcggtgat ttgcgtcccg
    17521 ccaaacaagc ctttcattaa ttcgctacgc cccgccccta ccagaccaaa cagcccaaca
    17581 atttcaccac tgcgaaccgc cagacttatt ggcgtacgca cgcctggtgc tttcacagca
    17641 tcaagacgca ggcgctcctc gccataacta cgaggttgcc agccgtagat atcgccaatg
    17701 tcgcgcccga ccatcgcctg caccagcgcg tcgtggtcaa cctgctgcat atcggtaaag
    17761 gttttgacat aacgtccatc tttaaagaca gtgatggcat cgctgagggc aaatatttct
    17821 tccatacggt gagaaacgta taagatcacc cgcccctctt ttcgcagttc acgaataacg
    17881 cggaaaagat tgtcgatttc gcgggcagaa agggagctgg ttggctcatc aaaggcgata
    17941 attttagcgt tacgcgccag cgccttggcg atttcaacca tctgccactg accaatggag
    18001 agatatttca gcggcgtgtc cgggtcaata tccataccaa gatgtttaag ttgtaaaccc
    18061 gcctcataat tcagcaatga gcgattcaca atgccgcctt tatgcggcag ctggccgaga
    18121 tagatgtttt ccgcgacggt catttccggc acgagatgca gttcctggta aataatcgcc
    18181 acacccgcgt taagtgctgc ggtcgtgtcg gaaaaggaca tttcctgccc attaatcact
    18241 acagaacccg tggttggcgc atagttgccg ctgaggattt ttaagagagt tgattttcct
    18301 gcgccatttt cacccatcaa cgcatgaacc tgaccggcat agcagtcaaa actaatatcc
    18361 gtcagcgcct taacgccggg aaacgtttta ccgatgccgc gaaatgagag atacggggta
    18421 gactgttgca taacgtctcc gtgaatcact ggtcgttcac accccctctg catcatgcag
    18481 aggggaattt ttccggcaaa ttacttaccg cctaaacctt ttttctccag ttcttcttta
    18541 aagttgtcac gcgtgatcag caccacgtcg gtaacttcgg taaattttgg cggttcaacg
    18601 tcttttgcta cccagttgta aagcatttcg ctggatttat agccatgtac gtccgggctt
    18661 ggcagcaggg aaccgtagaa gccggttgcc tgtgctttag acagttcgct caacgcatcc
    18721 acaccgttaa tgccaatgcc gatgatatcg gcagctttaa agccctgacc ttccgtcgcg
    18781 cgtacgccgc ccagcacggt gctgtcgttc ataccgacga tcagccaatg tttaacttcc
    18841 ggatgttgaa ccagcattga gttggcagcg tcaaatgccc ccgggatgtc gttagatttg
    18901 gtaggtacct gataaatttg tttttccggg aatccggccg ctttcagcgc atccatagaa
    18961 cccgttgtac ggcgacgtgc cgtatccagt tcgttggcgg taatcgccat caccgcgctt
    19021 tctttgacat cccagccacg tttctgcatc tctttataca gttcctgacc ctgacgttcg
    19081 ccaattttag tcgccgccat catcaccagc ggaacggtat ccattggctt acctttggcg
    19141 ttaacaaact ggtcatccac ggcaatgact ttcatatcgt agccacgcgc tttcgcgacg
    19201 atggcagagc cgagtttggg gtccggagta caaataacga aaccttttgc gccactggca
    19261 gccaggctgt cgatcgcgtt caatgttttt tcgccatccg gcacggcaat cttaataacc
    19321 tcaaacccta aatccttccc ggctttatcg gcaaacttcc attcggtctg gaaccacggc
    19381 tcttccggtt gcttcaccag aaaaccgagc ttcaggttct ccgccatagc ggattgtgac
    19441 ataacggctg ccagaccaat ggctgccagg gctttagtaa atttgtgcat ggttctctcc
    19501 agctttagtg tcgttttgtg tagggcaaaa acgaatgact ttcgttaaat taatcggaaa
    19561 acaaagcatt accttttatc taaaagataa gtgaccgtgt tgacatagtt ttagcgagaa
    19621 attaattctc cataggagag caatatcaca tcgcagaatt acagtgagaa cgtgcataaa
    19681 tttagcggga aaagacataa gggaaagcca atttgtcaga caaattgtcg aatgcacagc
    19741 agattaatcc ataagattag cctggaaatc cttgttgtct ttggtaccca tgcgggatgt
    19801 cttcttttta accagtcaat aggccgcatt acctggcgtt gagtttttga aatggtgtaa
    19861 taaccgcaac tcaaagatgt ggaaaatgca cgtcattcat ttcgccatta attatcactg
    19921 tgctcattaa ttaacagaac acgtataatg agagccatct cgcaaaaatg aaaaaacgtt
    19981 ttataaaatc atcacttcat catgaattca aattcattga ttaatatcaa caagatacaa
    20041 aaagcactat cattaaaatt cattgcagtt acattgattt catcaatgaa atgtaaaaat
    20101 atataaactt gatgatttaa gcattttctt atacccgttc agacgttatt cttatttcag
    20161 atcatcgtca gaattgactc cacgatcaca tttcggaccg gcagaaagga attattctgc
    20221 aaacagtaat tatggtgttt tgatttatct tgcacctctc cacttctgga tataaggata
    20281 ttaggtatgg caaccgctgg aatgcttctc aaactcaact ctcaaatgaa ccgcgagttt
    20341 tacgcatcca atctctacct tcacctgagt aactggtgtt ctgaacagag tctgaacggc
    20401 accgccactt tccttcgcgc ccaggcacag agtaatgtga cccaaatgat gcgcatgttt
    20461 aactttatga agagtgtcgg cgctaccccc atcgttaaag ccattgatgt tcccggtgaa
    20521 aaactgaact ctctggaaga actgttccaa aaaacgatgg aagaatacga gcaacgttct
    20581 agtacgttgg cacagttagc cgatgaagcg aaagaactga atgatgattc aaccgtcaat
    20641 ttcctgcgcg atctggaaaa agaacagcag catgatggtc tgttgctgca aaccattctt
    20701 gatgaagtgc gcagtgcgaa acttgcgggt atgtgccctg tgcagaccga ccaacatgtt
    20761 ctgaatgtcg tgtcacacca gctgcattga tcatcatcgg cgctaatgca ttgcgccgat
    20821 gaaggttttg agaaaccgct gcctcatctg tttgaagcag cggttttttt aatgggattc
    20881 accctgtggg gtaaacttga gttcaataag cgcgatggct ttttggattg cccgcatggt
    20941 gaccgggtct gcggcggcgg gatggttagt aaagtcgata ttcttcagct gactggacat
    21001 tttttcacga acttcaacgg gcgcgattac atcgagaaca tccagaattt gtttgataac
    21061 caactggcaa gcaaccacat cagaaaccag ttcctgatcg gcattcagcg gctgggacat
    21121 cgtaaactcc tgatagcatt ttgaaagccg ttatagtagc ggcttcacat cttcagcgat
    21181 agtcacatcc accgtcatca ggacacaaaa aaacctgccg gagcaggttt tttgttatcg
    21241 gaacatattg cctggcggta cgtctttgaa cgtcttgcaa tagttattga acatactttt
    21301 caggattttg cgcagtttca tcgcggcact ccgaccattt gttatacagg tgttattgtc
    21361 tttgcgctta taatatgaca accatcacaa aaatcaatct ttatgtgata caaatcacat
    21421 aaatacccct ttaatgttat aaaaatgata atcaaaaaac agccccccta tttctgacac
    21481 ctacagatgg caagaaatag cgcctgccag gcgtcttttc cggccattgt cgcagcgctg
    21541 taacgcgtaa aatagtgctt tctcttactc ttctggctgg accatgagac ttctgattct
    21601 gactctttca ttaataacgc tggcggggtg tacggttact cgccaagccc atgtgagcga
    21661 agttgacgcg gcaacaggta ttgtacggtt ggtttatgac caggcttttt tgcagcatgc
    21721 ccataccgat cgttatgtca gtcgcgggat cgccgatcgc gcatgccagc aggaaggcta
    21781 tacccacgcg gtcccctttg gtcagccagt aggcaactgt agcctttttg cgggttcctt
    21841 atgtttgaat acggaattca ctttgtcata tcagtgccat cattccgcct tccctgtctt
    21901 tctctaaata ttttaatcag cgaggggatc ttcgctgatt aaagaaatag cggtaatgcg
    21961 ttttattccc a
//
LOCUS       DAKISA010000075        21772 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.075, whole genome shotgun sequence.
ACCESSION   DAKISA010000075 DAKISA010000000
VERSION     DAKISA010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21772
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.075"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..140)
                     /locus_tag="OVW45_002847"
     CDS             complement(<1..140)
                     /locus_tag="OVW45_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5527250.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAV"
     gene            complement(186..527)
                     /locus_tag="OVW45_002848"
     CDS             complement(186..527)
                     /locus_tag="OVW45_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5527251.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(696..2150)
                     /gene="xylB"
                     /locus_tag="OVW45_002849"
     CDS             complement(696..2150)
                     /gene="xylB"
                     /locus_tag="OVW45_002849"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCV5527252.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVDSQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESVEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(2222..3544)
                     /gene="xylA"
                     /locus_tag="OVW45_002850"
     CDS             complement(2222..3544)
                     /gene="xylA"
                     /locus_tag="OVW45_002850"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HCV5527253.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            3892..4617
                     /gene="xylF"
                     /locus_tag="OVW45_002851"
                     /pseudo
     CDS             3892..4617
                     /gene="xylF"
                     /locus_tag="OVW45_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
     gene            4695..6236
                     /gene="xylG"
                     /locus_tag="OVW45_002852"
     CDS             4695..6236
                     /gene="xylG"
                     /locus_tag="OVW45_002852"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCV5527254.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKL
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            6214..7395
                     /gene="xylH"
                     /locus_tag="OVW45_002853"
     CDS             6214..7395
                     /gene="xylH"
                     /locus_tag="OVW45_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCV5527255.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITDILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNRMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            7473..8645
                     /gene="xylR"
                     /locus_tag="OVW45_002854"
     CDS             7473..8645
                     /gene="xylR"
                     /locus_tag="OVW45_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
                     /protein_id="HCV5527256.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARAWHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYHSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISNLEKRFKEEVGETIHAMIHA
                     EKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEIML"
     gene            complement(8753..9577)
                     /locus_tag="OVW45_002855"
     CDS             complement(8753..9577)
                     /locus_tag="OVW45_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCV5527257.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKTFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSCAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            9897..11927
                     /gene="malS"
                     /locus_tag="OVW45_002856"
     CDS             9897..11927
                     /gene="malS"
                     /locus_tag="OVW45_002856"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCV5527258.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLINKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCIAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVVWAGQQ"
     gene            12105..13358
                     /gene="avtA"
                     /locus_tag="OVW45_002857"
     CDS             12105..13358
                     /gene="avtA"
                     /locus_tag="OVW45_002857"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCV5527259.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEAQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(13509..13982)
                     /locus_tag="OVW45_002858"
     CDS             complement(13509..13982)
                     /locus_tag="OVW45_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCV5527260.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(14084..14932)
                     /gene="yiaJ"
                     /locus_tag="OVW45_002859"
     CDS             complement(14084..14932)
                     /gene="yiaJ"
                     /locus_tag="OVW45_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCV5527261.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            15133..16131
                     /gene="yiaK"
                     /locus_tag="OVW45_002860"
     CDS             15133..16131
                     /gene="yiaK"
                     /locus_tag="OVW45_002860"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCV5527262.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            16143..16610
                     /locus_tag="OVW45_002861"
     CDS             16143..16610
                     /locus_tag="OVW45_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCV5527263.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGQEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
     gene            16728..17201
                     /gene="yiaM"
                     /locus_tag="OVW45_002862"
     CDS             16728..17201
                     /gene="yiaM"
                     /locus_tag="OVW45_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HCV5527264.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            17204..18415
                     /gene="yiaN"
                     /locus_tag="OVW45_002863"
     CDS             17204..18415
                     /gene="yiaN"
                     /locus_tag="OVW45_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HCV5527265.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMANLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVL"
     gene            18495..19481
                     /gene="yiaO"
                     /locus_tag="OVW45_002864"
     CDS             18495..19481
                     /gene="yiaO"
                     /locus_tag="OVW45_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter
                     substrate-binding protein YiaO"
                     /protein_id="HCV5527266.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLTPEFQQALISSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGNQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            19485..20981
                     /gene="lyxK"
                     /locus_tag="OVW45_002865"
     CDS             19485..20981
                     /gene="lyxK"
                     /locus_tag="OVW45_002865"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HCV5527267.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRALLTHSGVSGEQIVGIGISAQGKGLFLLDKNNKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGEFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTVHQLYQQKYQRYQHLIAALQGFHARIKEHIL"
     gene            20978..21640
                     /gene="ulaD"
                     /locus_tag="OVW45_002866"
     CDS             20978..21640
                     /gene="ulaD"
                     /locus_tag="OVW45_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCV5527268.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTQLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGSANPAQVAGDFHAQIDAIWGGK
                     RA"
     gene            21633..>21772
                     /locus_tag="OVW45_002867"
     CDS             21633..>21772
                     /locus_tag="OVW45_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462576.1"
                     /note="L-xylulose 5-phosphate 3-epimerase activity not yet
                     demonstrated; may be involved in the utilization of
                     2,3-diketo-L-gulonate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose 5-phosphate 3-epimerase"
                     /protein_id="HCV5527269.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSR"
BASE COUNT         5418 a         5536 c         5429 g         5389 t
ORIGIN      
        1 acggcaaaat aatacccttt ttcatttaac tgcatctctg cattccatag ccctaacaga
       61 taggtaacga taccaccaac gagagctatc catgacacaa tactaaaggc cggtgaatag
      121 gttgatattt tgttgtccat cacagtattc ctttcattcc tgaatgtgta agagctttat
      181 gttgctcata ccgagcgaaa caaacgcatt tgaccaaaca agcagacaaa aaaggcgatg
      241 gggtagatca ccatttcata agcattgata ggtgcattta aaacaccgat aaacaagaga
      301 ccgatagtga ttaacgcaac taactggcag atatgggtaa aaaaattatc cagttgcccg
      361 agtttttctg cgcgaagata tgcatagtta ccaaaagttg ctgtcattaa cacgccaaga
      421 aaatagcctt ttccacttag caatgggcat gagatccata gcccaaccag ataaaccagc
      481 gccccgacaa caaataatag ttttgccaca gtttttgagg ttttcataac gatctccata
      541 tctaccagcg atacattacg agtaaccaac gaaagacaaa actgaaaaat gccattaaca
      601 aatgattttc agaataaatt catactaaat attaattaat tagtgagata tatagatgtg
      661 aattatcccc cacccggtca ggcaggggat aacgtttacg ccattaatgg cagaagttgc
      721 tgatagaggc gacggaacgt ttcacgtcgt ggctgataag cggcataacg ctgcgcatct
      781 ggtagatgcg actgttctaa cggtagctgc ggcaacaact caatgagcga tttctctgga
      841 ttcgccgcaa tctgcgccag ccttgctgcg cccagtgctg gccccacatc cccccccgta
      901 cggtaatcga gctgctgacc gctgatatcc gccagcatct gacgccagta ctcactacgc
      961 gccccgccac caatcaacgt aacactttgc ggtttaatgc cgcaggcatg cacgacatcc
     1021 atgccatctg ccagcgcata acccacgcct tccagcactg ctcgcgccag ttcattgggg
     1081 ccatgttgat gagtcaaacc aaagaaaacc cccttcgcct ggggattatt gtgtggtgta
     1141 cgctcgccgg aaagataagg cagaaaccaa actggctcga cactttcatc agcctgttga
     1201 gctgcagcga ttaaagctgg gacattgcac aggccggtta atttcgcggc ccaatccaga
     1261 cacgatgctg cactcagcat tacagacatt aaatgccaac gctgcggtag cgcatggcaa
     1321 aagctgtgta cagcgctttc tggcttgctt aagaaccctt cgctgacagc aaaatagacc
     1381 cccgacgtcc ccagcgataa cattgcctga ttagcatcaa ccattcccac accaactgca
     1441 ccagctgcat tgtcgccacc gcctgcgaca actggcaccg tcgccatacc ccacgctttc
     1501 gcaacttcag gtaacaaagc accagtaatt tcgctgcctt cgtataatgc gggcatctgg
     1561 tcacgagata agtcgcaagc ctgcagcatg acgtcactcc agtcacgctt tgcgacatcc
     1621 agccacatgg tgccagctgc gtcagacata tcgctggcaa actcccccgt catacgcaga
     1681 cgcaagtaat ctttcggtaa taatactttg tcgatttgac ggaatatctc cggctcatgc
     1741 cgctgaaccc atagcaattt aggcgcagta aatccgggca tcatcaggtt gccggtaatc
     1801 actcgtgatt gcggaactct cgcttccagc aaagtgcact cttgcgcaca gcgcccgtcg
     1861 ttccacaaaa tggcagggcg caatacccgt tgttgagcat ccagtaaggt tgctccatgc
     1921 atctggccgg caatacccaa tgctttaacg tcctgcagag aatgctgatc gcccagagct
     1981 ttcattgcgc gatcagttgc ctgccaccac tgttccgggt cttgttccga ccagagtgga
     2041 tgcgggcgcg aaacggtcag cttttccgtt tgcgaatcaa ccacctcacc ctgctcgttg
     2101 agcaaaataa cttttacgcc cgaggtgcca agatctatcc cgatatacat atcgatcgtt
     2161 ccttaaaaaa atgcccggta tcgctaccga taaccgggcc aacggactgc acagttagcc
     2221 gttatttgtc gaacagataa tgatttacca gattttccag ttgctcctgg cgaccactct
     2281 gatgcaccgg agacaaatta tgttcctgag catatttggc taaatctgcc agtgacattt
     2341 ggcctttcag gatttgctgg cccaattcgc tattccagcc ggaataacgc tgcgcgatgc
     2401 gtttatccag ctcgccatct tcaatcatgc gcgctgcaat tttcagcgcc agtgccatcg
     2461 tatccatcgc gccgatatga ccgtaaaaca gatcatattt atcagtactt tgacgacgta
     2521 ctttggcatc gaagttcaga ccaccggtgg tgaaaccgcc tgctttgaga atttcataca
     2581 tcaccagcgc attctcttcc acactgttcg ggaactggtc ggtgtcccag cccagttgcg
     2641 catcgccacg gttggcgtcg acagaaccga acaggccaag cgcaatggcg gtggctattt
     2701 catgatggaa agagtgacct gccagcgtcg cgtggttagc ttcaatgttc agtttaatct
     2761 ctttttccag accaaactgt ttcaggaagc catagaccgt cgcggcatcg taatcatatt
     2821 gatgtttagt cggttcttgc ggtttcggtt cgataagcaa cgtgccctgg aagccgattt
     2881 tatgtttatg ctcaaccacc atctgcataa agcggcccag ttgttcacgc tcctgacgca
     2941 agtcggtatt taacagcgtt tcgtaacctt cacgaccacc ccacaggaca tagttttcac
     3001 cgcccaattt atgggttgct tccatcgctg taacaacttg cgttgccgcc cagctgaaga
     3061 cttcaggatc tgggttcgtc gccgcacccg cgccatagcg agggtttgta aagcagttag
     3121 cggttcccca cagcagcttc acgccgctct cttcttgctt ggctgccagg acatcaacca
     3181 tttgcgcaaa attattgatg tactctttta acgacgcgcc ctcaggggaa acatccacat
     3241 cgtggaagca ataaaatggc acatgtaact tgtggaaaaa ctcaaatgcg acatctgctt
     3301 tacgcttcgc caacgccagt gcctcaccag gctgctgcca cggacgatta aacgccccca
     3361 caccaaacat gtccgccccg ttccagcaga aggtgtgcca gtagcaggcg gcaaaacgca
     3421 agtgctcttc catacgctta cccaacacca gttcgtcggg attgtagtga cggaatgcta
     3481 acgggtttga ggattttgag ccttcataac gaacgcgatc gagctggtca aaataggctt
     3541 gcatattgaa ctccataatc aggtaatgcc gcgggtgatg gatgatgtcg taatattggg
     3601 cactcccttt cagttgctca attatgttat ttcacactgc tattgagata attcacaagt
     3661 gtgcgctcgc tcgcaaaata aaatgggatg atgaaactgg gtaattcctc gaagataaaa
     3721 atgcaataag tacaattgcg taacaaaagt aagatctcgg tcataaatca agaaataaac
     3781 caaaaatcgt aatcgaaaga taaaaatctg taattgtttt cccctgttta gttgctaaaa
     3841 attggttacg tttatcgcgg tgattgttac ttattaaagc tgtcctctaa ctacaacggc
     3901 caggtattaa gtaacgttgt aaaagaagcc aaacaagaag gtattaaagt attagcttac
     3961 gaccgtatga ttaacgatgc ggatatcgat ttttatattt ctttcgataa cgaaaaagtc
     4021 ggtgaactgc aggcaaaagc cctggtcgat attgttccgc aaggtaatta cttcctgatg
     4081 ggcggctcgc cggtagataa caacgccaag ctgttccgcg ccggacaaat gaaagtgtta
     4141 aaaccttacg ttgattccgg aaaaattaaa gtcgttggtg accaatgggt tgatggctgg
     4201 ttaccggaaa acgcattgaa aattatggaa aacgcgctaa ccgccaataa taacaaaatt
     4261 gatgctgtag ttgcctcaaa cgatgccacc gcaggtgggg caattcaggc attaagcgcg
     4321 caaggtttat cagggaaagt agcaatttcc ggccaggatg cggatctcgc aggtattaaa
     4381 cgtattgctg ccggtacgca aactatgacg gtgtataaac ctattacatt gctggcaaat
     4441 actgccgcag aaattgccgt tgaattgggc aatggtcagg agccaaaagc agatacctca
     4501 ctgaataatg gcctgaaaga tgtcccctcc cgcctcctga caccgatcga tgtgaataaa
     4561 aacaacatca aagatacggt aattaaagac ggattccaca aagagagcga gctgtaagcg
     4621 ttacgccccg gcgcggagcg ggggcgtgat ttctctccat gccgcgtgaa tgaattggct
     4681 taggtggagt cgttatgcct tatctacttg aaatgaagaa cattaccaaa accttcggca
     4741 gtgtgaaggc gattgataac gtcagcttgc ggttgaatgc tggcgaaatc gtctcactct
     4801 gtggggaaaa tgggtctggt aaatcaacgc tgatgaaagt gctgtgtggt atttatcccc
     4861 atggctccta cgaaggcgaa attatttttg cgggagaaga gattcaggcg agtcacatcc
     4921 gcgataccga acgcaaaggt atcgccatca ttcaccagga attggccctg gtgaaagaat
     4981 tgaccgtgct ggaaaatatc ttcctgggta acgaaataac ccacaatggc attatggatt
     5041 atgacctgat gacgctacgc tgtcagaagc tgctcgcaca ggtcagttta tccatttcac
     5101 ctgatacccg cgttggcgat ttagggcttg ggcaacaaca actggttgaa attgccaagg
     5161 cacttaataa actggtgcgc ttgttaattc tcgatgaacc gacagcctca ttaactgagc
     5221 aggaaacgtc ggttttactg gatattattc gcgatctaca acagcacggt atcgcctgta
     5281 tttatatttc gcacaaactc aacgaagtca aagcgatttc cgatacgatt tgcgttattc
     5341 gcgacggtca gcacattggt acgcgtgatg ctgccggaat gagtgaagac gatattatca
     5401 ccatgatggt cgggcgagag ttaaccgcgc tttaccctaa tgaaccacat accaccggag
     5461 atgaaatatt acgtattgaa catctgacgg catggcatcc ggttaatcgt catattaaac
     5521 gagttaatga tgtctcgttt tccctgaaac gtggcgaaat actgggtatt gccggactcg
     5581 ttggtgccgg acgtaccgag accattcagt gcctgtttgg cgtgtggccc ggacaatggg
     5641 aaggaaaaat ttatattgat ggcaaacagg tagatattcg taactgtcag caagccatcg
     5701 cccaggggat tgcgatggta cccgaagaca gaaagcgcga cggcatcgtt ccggtaatgg
     5761 cggttggtaa aaatattacc ctcgccgcac tcaataaatt taccggtggc attagccagc
     5821 ttgatgacgc ggcagagcaa aaatgtattc tggaatcaat ccagcaactc aaagttaaaa
     5881 cgtcgtcccc cgatcttgct attggacgtt tgagcggcgg caatcagcaa aaagcgatcc
     5941 tcgcccgctg tctgttactc aacccgcgca ttctcattct tgatgaacct accaggggta
     6001 ttgatattgg cgcgaaatat gagatctaca aattaattaa ccaactcgtc cagcagggta
     6061 ttgccgttat tgtcatctct tccgaattac ctgaagtgct cggccttagc gatcgtgtac
     6121 tggtgatgca tgaagggaaa ctaaaagcca acctgataaa tcataacctg actcaggagc
     6181 aggtgatgga agccgcattg aggagcgaac atcatgtcga aaagcaatcc gtctgaagtg
     6241 aaattggccg taccgacatc cggtggcttt tccgggctga aatcactgaa tttgcaggtc
     6301 ttcgtgatga ttgcagctat catcgcaatc atgctgttct ttacctggac caccgatggt
     6361 gcctacttaa gcgcccgtaa cgtctccaac ctgttacgcc agaccgcgat taccgacatc
     6421 ctcgcggtag gaatggtgtt cgtcataatt tctgctgaaa tcgacctttc cgtcggctca
     6481 atgatggggc tattaggtgg cgtcgcggcg atttgtgacg tctggttagg ctggcctttg
     6541 ccacttacca tcattgtgac gttggttctg ggactgcttc tcggtgcgtg gaacggatgg
     6601 tgggtcgcgt accgtaaagt cccttcattt attgtcaccc tcgcgggcat gttggcattt
     6661 cgcggcatac tcattggcat caccaacggc acgactgtat cccccaccag cgccgcgatg
     6721 tcacaaattg ggcaaagcta tctccccgcc agtaccggct tcatcattgg cgcgcttggc
     6781 ttaatggctt ttgttggttg gcaatggcgc ggaagaatgc gccgtcaggc tttgggtttg
     6841 cagtctccgg cctctaccgc agtagtcggt cgccaggctt taaccgctat catcgtatta
     6901 ggcgcaatct ggctgttgaa tgattaccgt ggcgttccca ctcctgttct gctgctgacg
     6961 ttgctgttac tcggcggaat gtttatggca acgcggacgg catttggacg acgcatttat
     7021 gccatcggcg gcaatctgga agcagcacgt ctctccggga ttaacgttga acgcaccaaa
     7081 cttgccgtgt tcgcgattaa cggattaatg gtagccatcg ccggattaat ccttagttct
     7141 cgacttggcg ctggttcacc ttctgcggga aatatcgccg aactggacgc aattgcagca
     7201 tgcgtgattg gcggcaccag cctggctggc ggtgtgggaa gcgttgccgg agcagtaatg
     7261 ggggcattta tcatggcttc actggataac cgcatgagta tgatggatgt accgaccttc
     7321 tggcagtata tcgttaaagg tgcgattctg ttgctggcag tatggatgga ctccgcaacc
     7381 aaacgccgtt cttgattttg ataaaaattt tctcaaagcc ggttacgtat taccggtttt
     7441 gagtttttgc atgattcagc aggaaaagaa ccatgtttac taaacgtcac cgcatcacat
     7501 tactgttcaa tgccaataaa gcctatgacc ggcaggtagt agaaggcgta ggggaatatt
     7561 tacaggcatc acaatcagaa tgggatattt tcattgaaga agatttccgc gcccgcattg
     7621 ataaaatcaa ggactggtta ggagatggcg tcattgccga cttcgacgac aaacaaatcg
     7681 agcaagcgct ggctgatgtc gacgtcccca ttgttggggt tggcggctcg tatcaccttg
     7741 cagaaagtta cccacccgtt cattacattg ccaccgataa ctatgcgctg gttgaaagcg
     7801 catttttgca tttaaaagag aaaggcgtta accgctttgc tttttatggt cttccggaat
     7861 caagcggcaa acgttgggcc actgaacgcg aatatgcatt tcgtcagctt gtcgccgaag
     7921 aaaagtatcg cggagtggtt tatcaggggt tagaaaccgc gccagagaac tggcaacatg
     7981 cgcaaaatcg gctggcagac tggctacaaa cgctgccacc gcaaaccggg attattgctg
     8041 ttactgacgc ccgggcgtgg catattctgc aagtatgtga acatctacat attcccgtac
     8101 cggaaaaatt atgcgtgatt ggcatcgata acgaagaact gacccgctat ctgtcgcgtg
     8161 tcgccctttc ttcggtcgct cagggcgcgc ggcaaatggg ctatcaggcg gcaaaactgt
     8221 tgcatcgatt attagataaa gaagaaatgc cgctacagcg aattttggtc ccaccagttc
     8281 gcgtcattga acggcgctca acagattatc actcgctgac cgatcccgcc gttattcagg
     8341 ccatgcatta cattcgtaat cacgcctgta aagggattaa agtggatcag gtactggatg
     8401 cggtcgggat ctccaatctt gagaagcgtt ttaaagaaga ggtgggtgaa accatccatg
     8461 caatgattca tgccgagaag ctggagaaag cgcgcagtct gctgatttca accaccttgt
     8521 cgatcaatga gatatcgcaa atgtgcggtt atccatcgct gcaatatttc tactctgttt
     8581 ttaaaaaagc atatgacaca acgccaaaag agtatcgcga tgtaaatagc gagatcatgt
     8641 tgtagcgatg atgagaattg tcggcgtcac atcagggaac tcagcacagc cacctgatgc
     8701 gagcaaaagc cttatccgac cgacaaatca gataaggctc gaggaaatga aattacatat
     8761 gcgccgcgat taaccgttgg ttatcctggt acattgcgaa caggtagttg ttataactct
     8821 tccctttggt cgagtagccc ttcagcttgt gaatcatcgc tgtggcagtc acttcctgat
     8881 ccgctttacg cagctgtgca cacgatttac ggaacgaaga gtaagccggg tgcgtattca
     8941 ggttagtgac ataggcgctc accgattctt tgacagaact aaactgtgag taccctttca
     9001 ctttacccgg cgcattggta caacgtcctt tcatgcattt catgccgaac agattgttat
     9061 tgttgcgcgc cagcttcgac gttccccaac cgctttctgc tgcagccatc gtcgccacca
     9121 tactggtagg gataatgtct acgcgttcaa gcaaggtatt ccacgggatt tttcgcgtat
     9181 taccagacca cttcaccttg tagcgtttgg cgatgtcttt cagacgcgca cgctcagcag
     9241 gtgaccattg gccctgatac tgttttgaaa tcagccagtt acgttctgca gtaatggctg
     9301 cattttggct ggtaatgtaa ggcattacgg tccggagaaa cgtctttttc cttggtgttc
     9361 cggaagggta ttttcgcaaa tcaggaagtg aactactctt tgcactattg cgagaatact
     9421 cttgtttact gcttacctgt ttattactag ctttagttaa gtgggacttt tgactcgctg
     9481 ttgttgtgtg cgtcttcgct aacacctcac tcgaaaacac cagagtgagt aacataagaa
     9541 tcatcgcccc atatcgtcgt atgggagtca aaatcatcag gtctcctggt cggatttaat
     9601 cattccaaca ccttatattt ttcacaaatt tgagagttga ttctcaaatc atatcaaaaa
     9661 tagctgtcaa gagcacccca aggaatagtc caaatctgaa actatgtcac gagttaacga
     9721 ttcagattgg cgctaaatcg cagaaaatgt gggggttatc gcaaaattca gccgtttttt
     9781 gcgcgcgatt gctcaccctt gcttctcatc ctgtggactt accgctcagg gatgagtttt
     9841 gtctggctta tcgctggcaa actgtctgaa atcgcagcaa taaggactca tccaccatga
     9901 aactcgccgc ctgttttctg acactccttc ctggcttcgc cgttgccgcc agctggactt
     9961 ctccggggtt ccctgccttt agcgaacagg gaacgggaac atttgtcagc cacgcgcagt
    10021 tgcccaaagg tacgcgtcca ctcacgctaa attttgacca acagtgctgg cagcctgcag
    10081 atgcgataaa actcaatcag atgctttccc tgcaaccttg tagcaacacg ccgcctcaat
    10141 ggcgattgtt cagggacggc aaatatacgc tgcaaataga cacccgctcc ggtacgccaa
    10201 cattgatgat ttccatccag aacgccgccg aaccggtagc aaacctggtc cgtgaatgcc
    10261 cgaaatggga tggattaccg ctcacgctgg atgtcagcgc cactttcccg gaaggagccg
    10321 ccgtacggga ttattacagc cagcaaattg cgatagtgaa gaacggtcaa ataacgttac
    10381 aacccgctgc taccagcaac ggtttactcc tgctggaacg ggcagaaact gacacatccg
    10441 cccctttcga ctggcataac gccacggttt actttgtgct gacagatcgt ttcgaaaacg
    10501 gcgatcccag taatgaccag agttacggac gtcataaaga cggtatggcg gaaattggca
    10561 cttttcacgg cggcgattta cgcggcctga tcaacaaact ggattacctc cagcagttgg
    10621 gcgttaatgc tttatggata agcgccccat ttgagcaaat tcacggctgg gtcggcggcg
    10681 gtacaaaagg cgatttcccg cattatgcct accacggtta ttacacacag gactggacga
    10741 atcttgatgc caatatgggc aacgaagccg atctacggac gctggttgat agcgcacatc
    10801 agcgcggtat tcgtattctc tttgatgtcg tgatgaatca caccggctat gccacgctgg
    10861 cggatatgca ggagtatcag tttggcgcgt tatatctttc tggtgacgaa gtgaaaaaaa
    10921 cgctgggtga acgctggagc gactggaaac ctgccgccgg gcaaacctgg catagcttta
    10981 acgattacat taatttcagc gacaaaacag gctgggataa atggtgggga aaaaactgga
    11041 tcagaacgga tatcggcgat tacgacaatc ctggatttga cgatctcact atgtcgctgg
    11101 cctttttgcc ggatatcaaa accgaatcaa cgaccgcttc tggtctgccg gtgttctata
    11161 aaaacaaaac ggatacccac gctaaagtca tcgacggctt tacccctcgc gattacttaa
    11221 cccactggtt aagtcagtgg gtccgcgact atgggattga tggttttcgg gtcgataccg
    11281 ccaaacatgt tgagttgccc gcctggcagc aactgaaaac cgaagccagc gccgcgcttc
    11341 gcgaatggaa aaaagctaac cccgacaaag cattagatga caaacctttc tggatgaccg
    11401 gtgaagcctg gggccacggc gtgatgcaaa gtgactacta tcgccacggc ttcgatgcga
    11461 tgatcaattt cgattatcag gagcaggcgg cgaaagctgt cgattgtatt gcgcagatgg
    11521 atacgacctg gcagcaaatg gcggagaaat tgcagggttt caacgtgttg agctacctct
    11581 cgtcgcatga tacccgtctg ttccgtgaag ggggcgacaa agcagcagag ttattactat
    11641 tagcgccagg cgcggtacaa atcttttatg gcgatgaatc ctcgcgtccg ttcggtccta
    11701 caggttctga tccgctgcaa ggtacacgtt cggatatgaa ctggcaggat gttagcggta
    11761 aatctgccgc caacgtcgcg cactggcaga aaatcagcca gttccgcgcc cgccatcccg
    11821 caattggcgc gggcaaacaa acgacacttt cgctgaagca gggctacggc tttgttcgtg
    11881 agcatggcga cgataaagtg ctggtcgtct gggcagggca acagtaactt ctccggcttc
    11941 ccgttcgtca gtacctcggg aagccgccaa ccaggataaa atgtcagccc taatcagcgt
    12001 tgcaggataa agcaccgctc actcttcaac agaccgattt gcaccccagc aaatgtagcg
    12061 ttattgttac cttccttgct acagagttcg acagatatcc cgctatgaca ttctcccttt
    12121 ttggtgacaa atttacccgc cactccggca ttacgctgct gatggaagat ctgaacgacg
    12181 gtttacgcac gcctggcgcg attatgctcg gcggcggtaa tccggcgcag atcccggaaa
    12241 tgcaggacta cttccagacg ctactgaccg acatgctgga aagtggcaaa gcgactgatg
    12301 cactgtgcaa ttacgacggt ccacagggga aaacggagct actcacactg cttgccggaa
    12361 tgctgcgcga gaagttgggt tgggatatcg aagcacagaa tattgcacta acaaacggca
    12421 gccagagcgc gtttttctac ttatttaatc tatttgccgg acgccgtgcc gatggtcggg
    12481 tcaaaaaagt gctgttcccg ctggcaccgg aatacattgg ctatgctgac gccggactgg
    12541 aagaagatct gtttgtctct gcgcgtccga atattgaact gctgccggaa ggccagttta
    12601 aataccacgt cgattttgag catctgcata ttggcgaaga aaccgggatg atttgcgtct
    12661 cccggccgac gaatccaaca ggcaatgtga ttactgacga agagttgctg aagcttgacg
    12721 cgctggcgaa tcaacacggc attccgctgg tgattgataa cgcttatggc gtcccgttcc
    12781 cgggtatcat cttcagtgaa gcgcgcccgc tatggaatcc gaatatcgtg ctgtgcatga
    12841 gtctttccaa gctggggctg cctggctccc gctgcggcat tatcatcgcc aatgaaaaaa
    12901 tcatcactgc catcaccaat atgaacggca ttatcagcct ggcacctggc ggtattggtc
    12961 cggcgatgat gtgtgaaatg attaagcgta acgatctgct gcgcctgtct gaaacagtca
    13021 tcaaaccgtt ttactaccag cgtgttcagg aaactatcgc catcattcgc cgctatttac
    13081 cggaaaatcg ctgcctgatt cataaaccgg aaggagccat tttcctctgg ctatggttta
    13141 aggatttgcc cattacgacc gagcagctct atcagcgcct gaaagcacgc ggcgtgctga
    13201 tggtgccggg gcacaacttc ttcccgggac tggataaacc gtggccgcat acgcatcaat
    13261 gtatgcgcat gaactacgta ccagagccgg agaaaattga ggcgggggtg aagattctgg
    13321 cggaagagat agaaagagcc tgggctgaaa gtcactaaaa attgcctgat gcgctacgct
    13381 tatcaggcct acatgatttc tgcaatatat tgaatttgga agaatttgta ggccggataa
    13441 ggcgtttacg ccgcatctgg cataaacaaa gcgcactatt ccggcgttga gaaacgccgg
    13501 aaaacgtttc aaacaggctg ctgccgtagc cgatgccgct gcactttctc gacatccatg
    13561 cactgcaacg cctttgtcgg gcaggcttca acacaggccg gccccgtctc ccgatgccag
    13621 cacagatcgc acttaatcgc cctcgccttt ttgcgcgaag aaacgacctc catcgcacca
    13681 aacgggcaag ccagcataca gcttttacag ccaatgcaac gtgtttgttc aacgaaaata
    13741 tgcccatgtt cgcggcttat cgcgtcaaca gggcagacat tcgcgcacgg tgcatcttca
    13801 cactgatgac aggctactgc cgtggtccag ctgtggtctt taatgacacg aatacgggaa
    13861 ataaactcgt ctggtgacaa cgcagcgcaa tcctgattct cctgatgcga cactgcgcaa
    13921 gccacttcac aggtacggca accgatacat ttcgtcgcat ccgcaataat aaaccggttc
    13981 atgatcactc ctttgcacta aaacagcaga gtgccagaga gcgcgtccct actgctttga
    14041 tccaacgaag gaaatgttaa agttttggca cttgtccaaa gcgttatgtg atcgcgccca
    14101 ggtcatccct gacagtaaat cccagttcat tagaaatagc ctgcgcggtt tcacgcagtg
    14161 gtttcaggag atttttctct cccacctgtt tcagacgtga tgtcgaaagc gaaatcgaca
    14221 cggcgtacgg cacccgccca tgaatatcaa acaccggaac agcaatacag gagacgccga
    14281 gttcgttttc ttctctgtcc atcgccgctc cgctttcacg aatgtgcgcc agttcgtcga
    14341 acatcgcggg cagctcggta atggtattgc gggttaacgg ctggatctca tgctgatggt
    14401 tttcccagta tgacttcacg tagtccgggt gaccaaacgc catgtagatc ttgcccattg
    14461 cggaacagta gagcggcata tgctggccaa tataggcacg ggttcgcagc atcccggttg
    14521 tgggttccag cttataaatc aaaatagcgt gatcgtcttc gcggctggag aagttaatgg
    14581 tttcaccagt ggcgatgttc agtgcctcaa gatgcggagc ggcgatatga atgatattca
    14641 gcgaagacag cgccttctgc ccgacggcaa taaatttggt agtcaggcgg taactccctg
    14701 cggcgggcgc ggtggtcaca tagccgcagg actgtaatcc ctgcaataag cgatggacgg
    14761 tactcttatt taaaccagcc agctccgaaa gatgcgccaa cggacaaccg tttggatagt
    14821 tgctcaaaat ctcaatcagc atcaacccgc gaaacagact ctggcttccg gctggacgct
    14881 ctttttcctg cgccatctcg ttctcttttt ttcccatcac ttctttcccc attttgtcgc
    14941 gtcctgatgg tagcgcaaag tgtgccgtag ttcacgatct cgacagataa tttataacca
    15001 tttgattttt atgtcttttg aaattcatca atcagattgc tttgttaaaa agtgatcgat
    15061 atatttgaaa tcaagtttcg catattgaaa ttttaagcca aaaaagcgat caaaaaaaca
    15121 agggagcctg ggatgaaagt gacatttgag cagttaaaag cagcctttaa tcgggtctta
    15181 atttcacgcg gcgttgacag cgaaacggct gacgcctgtg cagagatgtt cgcccgcacc
    15241 accgaatccg gcgtttattc tcacggcgtt aatcgtttcc ctcgtttcat tcaacaactg
    15301 gaaaacggcg atatcattcc tgatgcccaa cccaaacgta taaccagcct cggcgcaatt
    15361 gaacagtggg atgcccagcg ttcgatcggc aacctgacgg cgaaaaagat gatggatcgc
    15421 gccattgaac tggctgccga tcacggtatt ggtctggtgg cacttcgtaa tgccaaccac
    15481 tggatgcgcg gcggcagcta cggctggcag gcggcggaaa aaggctatat tggcatttgc
    15541 tggaccaact ccatcgccgt aatgccgccg tggggcgcaa aagagtgtcg catcggcact
    15601 aacccgctga tcgtcgccat tccttccacg ccgatcacca tggtcgatat gtcgatgtcg
    15661 atgttctctt acggcatgtt agaagttaac cgcctggcgg gccgtcagct cccggtcgat
    15721 ggtggctttg atgatgaggg caatttgacc aaagaacctg gcgttatcga gaagaatcgc
    15781 cgcattttgc cgatgggcta ctggaaaggt tctggcatgt cgattgtgct ggatatgatc
    15841 gctactctcc tttccgacgg cgcatcggtt gccgaagtca cccaggacaa cagcgacgaa
    15901 tacggcattt cacaaatttt tattgccatt gaagtggaca agcttatcga cggtcccacc
    15961 cgcgatgcca agctgcaacg tatcatggat tacgttacta ccgctgaacg cgctgacgaa
    16021 aaccaggcca tccgcttacc cggccacgaa tttactaccc tgctggccga aaaccgccgt
    16081 aacggcatca ctgttgatga cagcgtgtgg gccaaaatcc aggcgttata aggagataag
    16141 tcatgatttt tggacatatc gcgcagccca atccgtgccg tttgcccgcc gccattgaaa
    16201 aggcgctcga ttttctgcgc gctaccaact tcaacgtcct ggagccgggc gttgtcgaaa
    16261 tcgacggcaa gaatatttat gcgcaaatta tcgatttaac cacacgcgaa gcggtggaaa
    16321 accgtccgga agtgcatcgt cggtatatcg atattcagtt tctggcgtgg ggtcaagaga
    16381 aaatcggcat tgctattgat acaggaaata ataaagtcag cgaatcacta ttagagcagc
    16441 gcgatattat tttttatcat gacagtgaac atgaatcatt tatagaaatg ataccgggca
    16501 gctacgctat tttcttcccg caggatgttc accgaccagg ttgtattctg caaacagcct
    16561 ctgagatacg aaaaattgtg gttaaagtcg cattaacagc gctgaattaa tcaaataatc
    16621 gtctacgcca gaacgactgg gtaatgttat tgctctaact atattaataa tttaggttgc
    16681 tgcacatgca acttgaatta tgacagacat agcctcaagg aatagctatg aaaaaaatac
    16741 ttgaagcaat actggcgatt aatctcgccg tactttcctg tattgtgttt ataaacatta
    16801 ttttaagata tggttttcag acaagcattt tatctgtaga tgaattgtca cgttatttat
    16861 ttgtctggtt aacatttatt ggcgcgattg tggcttttat ggataacgcc cacgttcagg
    16921 tcacctttct ggtggagaaa ctctcccccg catggcaacg acgagtcgct ctggttaccc
    16981 attccttaat cttatttatt tgtggcgcac tggcctgggg cgcaacgcta aaaacaattc
    17041 aggactggag cgattattca ccgatcctcg gtttgcccat cggcctgatg tatgccgctt
    17101 gtctgcccac cagcctcgtt atcgcattct ttgaactgcg tcatttatat caactcatca
    17161 cgcgtagcaa ttctctaacg tcgccaccgc aaggagctta atcatggctg tgctgatttt
    17221 tctgggctgt ctgttgggtg gtattgctat cggtttgcct attgcctggg cactgttgtt
    17281 gtgcggggcg gcgttaatgt tctggctgga catgtttgat gtccagatca tggcacaaac
    17341 gctggtgaac ggagccgata gcttctccct gctggcgatt ccgttctttg tgctggcggg
    17401 tgaaatcatg aatgcgggcg gcttgtcaaa acgcattgtt gacctgccga tgaaactggt
    17461 ggggcataaa ccaggcgggc tgggctacgt cggcgtgctg gcagctatga ttatggcgaa
    17521 cctttctggt tctgccgttg cggataccgc cgccgttgcc gctctactgg tgccgatgat
    17581 gcgcagcgcc aactacccgg tcaaccgggc ggcggggctg attgcctctg gcggcattat
    17641 cgcgccaatt attccccctt ccattccgtt tattatcttc ggcgtttcca gcggattatc
    17701 catcagtaag ctgtttatgg cgggcattgc ccctggcatg atgatgggcg caacgctgat
    17761 gcttacctgg tggtggcagg ccagtcgcct taatttaccc cgccagcaaa aagcaacgat
    17821 gcaggaaatc tggcactctt ttgtctccgg tatctgggcg ctgtttcttc cagtcatcat
    17881 tattggcggg tttcgctccg gtctgtttac ccctaccgaa gccggggccg ttgctgcttt
    17941 ctatgcgctg tttgtcgcca cagttattta ccgtgaaatg accttcgcca cactttggca
    18001 tgtactcatt ggcgcggcga aaaccacctc agtggttatg tttctggtgg cctcggcaca
    18061 agtttccgcc tggctgatta ccattgctga actgccgatg atggtttctg atttactgca
    18121 accgttggtc gattcaccgc gtctgctgtt tatcgtcatt atggtggcta tcctgattgt
    18181 cggcatggtc atggatttaa cgccgacggt attaattctt accccggtat taatgccttt
    18241 agttaaagag gcaggaatcg atccgattta tttcggtgtc atgtttatca ttaactgctc
    18301 aatcggctta attacaccgc ctatcggtaa tgtacttaac gttatttcag gggtggcaaa
    18361 actcaaattc gatgatgcag tcagaggcgt tttcccttac gtcctggttt tataattcat
    18421 tattagtcgt atttgttttt attcccgatc tcatcatcct ccctttgaaa tggattaatt
    18481 aaaaggaaaa tattatgaaa ttacgctctg taacctacgc attattcatt gctggcctgg
    18541 ctgcattcag cacatcttct ctggcggcgc aatctttacg tttcggttat gaaacctcac
    18601 aaaccgactc gcaacatatt gcggcgaaaa aattcaatga tttattgcag gagagaacca
    18661 aaggcgagct gaaattaaaa ttgtttccgg atagcaccct cggtaacgcg caggcgatga
    18721 tcagcggcgt acgtggcggc accatcgata tggaaatgtc cggctcgaat aactttgccg
    18781 ggttatcacc agtgatgaac ttgcttgatg tccctttcct gttccgcgat accgctcacg
    18841 cgcataaaac gctcgacggc aaagtcggtg atgatctgaa agcctcactt gaaggtaaag
    18901 gactaaaagt actggcctac tgggaaaacg gctggcgcga tgtcaccaac tcgcgcgcac
    18961 cggttaaaac ccccgccgac ctgaaagggc tgaaaattcg caccaacaat agcccgatga
    19021 atatcgccgc attcaaagtc tttggcgcta acccgatccc gatgccgttt gccgaagtct
    19081 ataccgggct ggaaacccgc actatcgacg ctcaggaaca cccgatcaac gtcgtctggt
    19141 cagcaaaatt tttcgaagtg cagaagtacc tttctctgac ccatcacgcc tattccccac
    19201 ttctggtggt gatcaacaaa gcgaagtttg atggcttaac cccggagttc cagcaggcgc
    19261 tgatctcatc tgcgcaggaa gcgggtaact accagcgcaa actggttgcc gaagatcagc
    19321 aaaaaatcat cgacggcatg aaagaagcgg gcgtggaagt catcaccgat ctcgaccgca
    19381 aagcctttag cgacgctctg ggtaatcagg ttcgcgacat gtttgttaaa gatgtgccgc
    19441 agggagctga tctgctgaaa gccgtggatg aggtgcaata aaccatgacg caatactggc
    19501 tggggttaga ttgtggcggt agctggctga aagccgggct gtatgaccgc gaaggccggg
    19561 aggcaggcgt gcagcgcctg ccgctgtgcg cattaagccc gcagccaggt tgggcagagc
    19621 gcgatatggc agaactgtgg caatgctgca tggctgtcat tcgcgccctg cttacccatt
    19681 ccggcgttag cggggagcaa atagtgggta tcggtatctc cgcacaagga aaaggcttgt
    19741 ttttgctgga taaaaacaac aagccgctcg gtaacgccat tttgtcctcg gatcgccggg
    19801 cgatggaaat cgttcgtcgc tggcaggaag atggcatccc ggaaaaactc tacccgttga
    19861 cccgacaaac cttgtggacc gggcatccgg tgtcgctgtt acgctggctg aaagagcacg
    19921 aaccggaacg ctacgcgcaa attggctgcg tgatgatgac gcacgactac ctgcgctggt
    19981 gtttaaccgg cgtcaaaggc tgtgaagaga gcaatatttc cgagtccaac ctctacaaca
    20041 tgagtcttgg ggaatatgac ccgtgcctca ccgactggct ggggattgct gaaatcaatc
    20101 acgccctgcc gcctgttgtc ggatctgccg aaatctgcgg ggagatcacc gctcagacag
    20161 ccgtactgac cggtctgaaa gcgggtacgc ccgttgtcgg cggcctgttt gatgtggttt
    20221 ccaccgcact ctgcgccggg atcgaagacg aatttaccct taatgcggtg atgggcacct
    20281 gggcggtgac cagcggcata acccgcggtt tacgtgacgg tgaagcgcat ccgtatgtct
    20341 atggtcgcta cgttaacgat ggtgaattta tcgttcacga agccagcccc acctcttccg
    20401 gcaacctcga atggtttacc gcacagtggg gagaaatctc gtttgatgag atcaaccagg
    20461 ccgttgccag cttgccgaag gccggtggcg atctcttttt cctgccgttc ctgtacggca
    20521 gcaacgccgg actcgagatg accagcggtt tctacgggat gcaggccatt cacacccgcg
    20581 cacacctgtt gcaggccatt tatgaagggg tggtgttcag ccatatgacc catctcaacc
    20641 ggatgcgcga acgttttact gatgtgcaca ccctgcgcgt cactggcggc ccggcgcatt
    20701 ccgatgtctg gatgcaaatg ctggcggacg tcagcggtct gcgtattgag ctgccgcagg
    20761 tggaagaaac cggctgcttt ggtgcagcac ttgccgcccg cgtcggcacc ggggtttatc
    20821 gcgatttcag cgaagcccag cgtgatttac agcacccggt gcgcaccctg ctgccggata
    20881 tgactgtcca tcagctttac caacaaaaat accaacgcta tcagcatctc attgccgcac
    20941 ttcagggctt tcacgcccgc atcaaggagc acatattatg agccgaccac ttctgcaact
    21001 ggcactcgac cactcatcac ttgaagccgc acagcgcgac gtgacgcagt taaaagacag
    21061 cgtcgatatt gtcgaagcgg gcaccattct ctgtttaaac gaagggcttg gcgcggtgaa
    21121 agccttgcgc gaacagtgcc ctgacaaaat catcgttgcc gactggaagg tcgccgacgc
    21181 tggtgaaacg ctcgcgcaac aggcatttgg cgcaggcgct aactggatga ccatcatctg
    21241 cgccgcaccg ctcgcgacgg tggaaaaagg ccacgcgatg gcgcaacgct gcggtggtga
    21301 aattcagatc gagctgttcg gtaactggac gctggacgac gcccgcgact ggcatcgtat
    21361 tggcgtgcgg caggcgattt atcatcgcgg tcgtgatgca caggccagcg ggcaacagtg
    21421 gggcgaagcc gatctggcac gcatgaaggc gctttccgat atcggccttg agctttccat
    21481 taccggcggg attacccctg ctgacctgcc gctgtttaaa gatattcgcg tgaaagcgtt
    21541 tattgccggg cgagcactgg caggctcagc aaatccggcg caggtggctg gcgatttcca
    21601 tgcgcaaatc gacgctatct ggggaggcaa gcgtgcgtaa tcatccgtta gggatttatg
    21661 aaaaggcgct ggcgaaagat ctctcctggc cggagcggct ggtgctggca aaaagctgcg
    21721 gttttgattt tgtcgaaatg tcggtggatg aaaccgacga acggctttca cg
//
LOCUS       DAKISA010000076        21706 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.076, whole genome shotgun sequence.
ACCESSION   DAKISA010000076 DAKISA010000000
VERSION     DAKISA010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21706
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.076"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..1796
                     /locus_tag="OVW45_002868"
     CDS             <1..1796
                     /locus_tag="OVW45_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CS1-pili formation C-terminal domain-containing
                     protein"
                     /protein_id="HCV5527270.1"
                     /translation="GSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTK
                     TGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFE
                     LGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACT
                     TDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQSFENNYLR
                     QTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPT
                     MDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINS
                     AVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSCYGVNLGASGTDDLL
                     GAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSS
                     QGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLN
                     SAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTY
                     SQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            1793..2881
                     /locus_tag="OVW45_002869"
     CDS             1793..2881
                     /locus_tag="OVW45_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCV5527271.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGDFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(2964..4262)
                     /gene="exuT"
                     /locus_tag="OVW45_002870"
     CDS             complement(2964..4262)
                     /gene="exuT"
                     /locus_tag="OVW45_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCV5527272.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            4745..6157
                     /gene="uxaC"
                     /locus_tag="OVW45_002871"
     CDS             4745..6157
                     /gene="uxaC"
                     /locus_tag="OVW45_002871"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCV5527273.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6172..7659
                     /gene="uxaA"
                     /locus_tag="OVW45_002872"
     CDS             6172..7659
                     /gene="uxaA"
                     /locus_tag="OVW45_002872"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCV5527274.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            7742..8293
                     /locus_tag="OVW45_002873"
     CDS             7742..8293
                     /locus_tag="OVW45_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCV5527275.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(8298..9542)
                     /gene="sstT"
                     /locus_tag="OVW45_002874"
     CDS             complement(8298..9542)
                     /gene="sstT"
                     /locus_tag="OVW45_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCV5527276.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10158..11123)
                     /gene="alx"
                     /locus_tag="OVW45_002875"
     CDS             complement(10158..11123)
                     /gene="alx"
                     /locus_tag="OVW45_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCV5527277.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11406..12392)
                     /locus_tag="OVW45_002876"
     CDS             complement(11406..12392)
                     /locus_tag="OVW45_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCV5527278.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLESEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(12471..13154)
                     /locus_tag="OVW45_002877"
     CDS             complement(12471..13154)
                     /locus_tag="OVW45_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCV5527279.1"
                     /translation="MQPRLLLRICFSRRTLKITCLLLLVAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHVGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(13231..13734)
                     /locus_tag="OVW45_002878"
     CDS             complement(13231..13734)
                     /locus_tag="OVW45_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCV5527280.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            13819..14955
                     /gene="rlmG"
                     /locus_tag="OVW45_002879"
     CDS             13819..14955
                     /gene="rlmG"
                     /locus_tag="OVW45_002879"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCV5527281.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15240..15554
                     /gene="higB"
                     /locus_tag="OVW45_002880"
     CDS             15240..15554
                     /gene="higB"
                     /locus_tag="OVW45_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCV5527282.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            15551..15967
                     /gene="higA"
                     /locus_tag="OVW45_002881"
     CDS             15551..15967
                     /gene="higA"
                     /locus_tag="OVW45_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCV5527283.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLADLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16012..18030)
                     /gene="fadH"
                     /locus_tag="OVW45_002882"
     CDS             complement(16012..18030)
                     /gene="fadH"
                     /locus_tag="OVW45_002882"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCV5527284.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18256..20607)
                     /gene="ygjK"
                     /locus_tag="OVW45_002883"
     CDS             complement(18256..20607)
                     /gene="ygjK"
                     /locus_tag="OVW45_002883"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCV5527285.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWLSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGLYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(20624..21701)
                     /gene="ygjJ"
                     /locus_tag="OVW45_002884"
                     /pseudo
     CDS             complement(20624..21701)
                     /gene="ygjJ"
                     /locus_tag="OVW45_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
BASE COUNT         5338 a         5669 c         5662 g         5037 t
ORIGIN      
        1 tgggatcgtt ttatctcaat agtggttcgc aatttattga taccagttcc tttccgccag
       61 gcagctacag cgtggcatta aaagtctacg aaaataacca actcacccgc accgagcttg
      121 tgccgtttac caaaaccggc ggtctgactg acggaaatgc gcaatggttc ttacaggcag
      181 gtaaaactac atcacaggtt tctgatgatg aaagttcagc ttatcagcta ggagtacgcc
      241 tgccattaca tccgcaatat gagctctacg cagggctggc gaatgccgat gatgtgagtg
      301 ctttcgagtt aggtaataac tggacggcag atttaggcgg ggtggggaat cttgcaatca
      361 gcgccagcgt gttccgtaac gatgacggcg gcaaaggtga tatgcaacag gccaactgga
      421 gtaatccggg atggccgacg ttgggctttt atcggaccaa ctctgacggc gatgcttgta
      481 caaccgacag cagagagagc tataacgcct taagctgtta tgaaagtatt tccgcgacgg
      541 tttcacagaa ttttgtcggc tggaatatga tgctgggtta tacccgcaca caaaataaca
      601 ctgatgatag tttgcgttgg gataaacagc agagctttga aaataactat cttcgccaga
      661 caacagcgca aagtatctct gaaactgtac aacttagcgc ttcccgcgct tttgtgatgc
      721 gtgactggat tttgagtacg tcagtcggtg ttttccatcg taatgacaac ggtggcgata
      781 acgacgacaa cggcttgtac ttatcgtttt cgttatctga cacgccaacg atggatagca
      841 ataacaacag ccattcaaca aatgtgtcta cggattatcg ttatagcgaa caggatggcg
      901 atcaaacgtc atggcagtta tcgcatacct tttataacga ttcattcagc cataaagaac
      961 ttggcgtaac cgtcggaggc ctgaacaccg ataccataaa cagcgcggtt aacgggcgtt
     1021 gggatgggca atacggaaat gtctacgcta ccgtatctga tagttatgac cgtaagaatc
     1081 atgatcatct ttcggccttt acgggcactt atagctctac gctggctgtc agttgctatg
     1141 gcgttaattt gggagccagt ggtacagacg atttgctggg tgcggtactg gtggatgtga
     1201 aaggcttctc tgaacaggat gaagagagtc aggatctgca actcgaagcg cgggtggcag
     1261 gcagccgaac tttgcagctt ggtcaaagtg acagtgtgtt gttcccttat cctggatttc
     1321 agtctggttt tgttgaggtt aacgacagta gccagggcaa tcagcagggg acaacaaaca
     1381 tcattaacgg tgcagggaat cgtgaattaa tgttgttgcc tggcaagctg cgctatcgcg
     1441 aagtgtctgc cagctttaac tacaactata tcggtcgttt attactgccg gcagcggtga
     1501 aaaaattccc gattgtgggg ctgaacagcg ccatgttact ggtagctgaa gatggcggat
     1561 ttacacttga aattaacggc agcgaaaaag agctctatct gctttccggg cagcaattcc
     1621 ttaagtgtcc actgagtgtt gtaaagaaac gcgccagcat tcgttacagc ggagatgtta
     1681 cttgtagtgt ggtgacttat tcacaattac cggagtcgat tcaggttcag gcacagttga
     1741 aacagcctaa attacgtgga aacgttcaga cggcgcaaag ggaggttgca ccatgagaaa
     1801 tcgattgatt gcggcgatat tgggcttgtt tggcacgctc actggcgttc aggcagctcc
     1861 tgacgtgacc agtgaaatta cgtatgattt ggcatctggt agagcggatt attacttctg
     1921 gaaggatgaa gcgtcggcag gaaataatgg atatatgtgg tatgaatgtt cgtatcctga
     1981 cctccaacaa acctgtacag ctaatggaaa tatatcgaca gtacaaatct atttaactga
     2041 acaacgcagt gggatgcgtt ggccggtaaa actcaaagga tttaaaacag ccattgtaag
     2101 tagcgatgaa gcgccgccag gatgcaaggg gggcaaaggg cttcagacga atcttaagga
     2161 ttctaataga tcttcatgta cagaagatgg tcaacattat tatatatacg atacaaagtt
     2221 tcttacgctc taccttgagc agacagagat gaagaatttg ccgattggtg gcgtctggaa
     2281 ggggaaagtt aaattacatt cgaacagccc ggcccaggac tatttcgcaa atattaccct
     2341 gaatacgctc gaccccaacc atattgacgt gttcttcccg gagttcgccc acgccacgcc
     2401 aagggtgcag ttagacttgc atccaacagg aagcgttaac ggcagcaact acgcgcaaga
     2461 tctgaccatg ttggacatgt gcctgtacga tggttttaac ggtaatgcca tcagttatga
     2521 aatcatgctc aaagatgaag ggcgaccagc tgcagggcgc agagacggtg acttctctat
     2581 ctatcgtcaa ggagggacca ccaccgacga gggagaacgc attgattacc gggtcaaaat
     2641 gtacaacccg gaaaccggtg ggcaaattga tgtgcgcaat aatgaaaata tggtctggaa
     2701 cagcattaac ctgaaacgtg tgcgtccggt ggtactgccc ggtattcgct atgccgtgat
     2761 gtgtgtgccg acacccttga ctctggcagt cgataaattc agcgtgatgg ataaacaggc
     2821 cgggtactac atgggcaaat tgtcagtaat ctttacgcct tccttgccaa ccatcaattg
     2881 accccgcaga ccagaaaaaa ctaaacaacg aagccgcctg ataaggcggc ttcgtcatta
     2941 caacttacgt taaacgttaa acgttaatgt tgcggtgcgg gatcgttatg tgtttcctgc
     3001 gccacctcaa tcgccggttt gttctgcaat acggtccaga taaccagcgc acccagcagg
     3061 tcaaatactg ccagaactgc gaacagcggg ctgaagccga tggtgtcagc cagtgcaccg
     3121 acaaccagcg caaacagcgt acttgccagc catgcggaca tcccggttaa accgttcgcc
     3181 gtcgccactt cgttacgacc gaacacgtca gaagagagcg taatcagcgc gccagacagt
     3241 gcctggtggg caaaaccacc gatacacagc agcataattg cgacatacgg gttggtgaac
     3301 agaccgatca tacccgggcc aatcatcagc acagcaccca gcgttacgac catcttacgg
     3361 gaaacgatca ggttcacacc aaaccaacgc tggaacagcg gcggcaggta accaccgagg
     3421 atacaaccga ggtcagcaaa cagcatcggc atccaggcga acatcgcgat ctctttcagg
     3481 ttaaagccgt aaactttaaa catgaacagc gggatccacg cgttaaaagt accccaggcc
     3541 ggttctgcca ggaaacgcgg cagtgcgata ccccagaact gacggttacg caggatctga
     3601 ccaacggaca ttttcttcgc cgtgctcacc tggtgctggg cttcctgacc attaataata
     3661 tagtcgcgtt cttcatcggt cagatgcttc tggtcgcgcg gatgtttata gaaaatcagc
     3721 catgccatcg cccagataaa gctcaatgca ccggagatga taaatgccat ctgccagctg
     3781 tgcatcacga ttgcccatac caccagcggc ggcgcaatca tcgcaccaat cgaagaacct
     3841 acgttaaagt agcctactgc gatggaacgc tctttcgccg ggaaccattc ggagctggct
     3901 ttcagacccg ccgggatcat cgctgcttcc gcggcaccga ccgcaccacg agcaacagcc
     3961 aggccacccc agctacctgc cagcgcggtt gcaccacaga acacggccca cagcacagca
     4021 aacattgcat aaccgatttt cgtacccagc acatccagta cataacctgc taccggttgc
     4081 atgaccgtat aagcagcaga ataggctgcg atgatatagg aatactgttg ggtggagatg
     4141 tttaactctt ccatcagagt tggcgcagct gccgccacag tgttacgcgt caggtaacca
     4201 agcacggtgc cgagcgtcac cagtgcgatc atataccaac gtaacccttt aattttacgc
     4261 atgtaaaacc tcatcgtttt gttatatccg caggaagcgc ggccatcatc ccgccagagg
     4321 ctgaagatgt tagttacgac cgggtaaccg aaaaaaaacc ggcacgcccc gaacgttgcc
     4381 atgaaagttt cgtgctaatt cggcttccgt accggttaac ccgattcatc taacaaatga
     4441 atgaatcggc aatttgcatt ccgtcgactt atgatttgcg acggcagaaa gataacttgt
     4501 catacaactt taaaaggtga gagccatcac aaatgtgcga atatttgtag ggacattacc
     4561 tgacgacagc gaggccagta ctggcgcggc ctgcagcgag atttatcact ttgagagtaa
     4621 tttttttaac tacgtttatt gatctaactc acgaaaatat cttcggactc tggaaattgg
     4681 tgtgataact ttgtcagcat cgcaccataa gcaagctagc tcactcgttg agaggaagac
     4741 gaaaatgact ccgtttatga ctgaagattt cctgttagat accgaatttg cccgccgtct
     4801 gtatcacgac tacgcaaaag accagccgat tttcgattac cattgccatt tgccgccgca
     4861 gcagattgcg gaagactatc gttttaaaaa cctgtatgac atctggctga aaggcgatca
     4921 ctacaaatgg cgcgctatgc gtaccaacgg tgtggccgag cgtctgtgta ccggtgatgc
     4981 atctgaccgt gaaaaatttg acgcctgggc ggcgactgtt ccgcacacta tcggcaaccc
     5041 gttataccac tggacgcacc ttgaactgcg tcgtccgttt ggtatcactg gcaaattgct
     5101 ttctccgtca actgccgatg aaatctggaa cgaatgtaac gaactgctgg cgcaggataa
     5161 cttctccgcg cgcggcatca tgcagcagat gaacgtgaaa atggtcggca ccaccgatga
     5221 cccgatcgat tctctggagc atcacgcaga gatcgccaaa gacggctctt tcaccatcaa
     5281 agtgctgccg agctggcgtc cggacaaagc cttcaacatc gaacaggcga cctttaacga
     5341 ctacatggcg aagctgggcg aagtttccga taccgacatt cgccgctttg ctgacctgca
     5401 aactgccttg accaaacgtc tggatcactt cgccgctcac ggctgtaaag tgtctgacca
     5461 tgcgctggac gtggtgatgt ttgctgaagc gaacgaagcg gaactggaca gcattctggc
     5521 gcgccgtctg gctggcgaaa ccctgagcga gcacgaagtg gcacagttca aaactgcggt
     5581 actggtgttc cttggtgccg aatatgcacg tcgcggctgg gtacagcagt accacattgg
     5641 cgcgctgcgt aataacaacc tgcgtcagtt caaactgctg gggccggatg taggctttga
     5701 ctccatcaac gaccgtccga tggcggaaga gttgtctaag ctgctgagca agcagaacga
     5761 agaaaacctg ctgccgaaaa ccattctcta ctgcctgaac ccgcgcgata acgaagtgct
     5821 gggcaccatg atcggtaact tccagggcga aggtatgccg ggcaaaatgc agttcggttc
     5881 cggctggtgg tttaacgatc agaaagacgg tatggaacgt cagatgaccc aactggcgca
     5941 gctcggtctg ctgagccgct ttgtcggtat gctgactgac agccgtagct tcctgtcata
     6001 cacccgtcac gaatacttcc gccgcattct gtgccagatg atcggtcgct gggtggaagc
     6061 gggcgaagca ccggcggaca tcaacctgct gggcgagatg gtgaaaaata tttgctttaa
     6121 caatgcgcgt gactacttcg ccattgaact gaactaaggt ctggggttga tatgcaatac
     6181 atcaagatcc atgcgctgga taacgtcgcg gtcgctttag cagatttggc tgaaggcaca
     6241 gaagtcagtg tcgataacca gactgttacg ctgcgccagg atgttgctcg tggacataaa
     6301 tttgcgttaa cggatatcgc aaaaggggcc aacgtcatca aatatggcct gccgattggt
     6361 tatgcattgg cggatattgc ggcgggggaa cacgtccacg cccacaatac gcgcacgaat
     6421 ctgagcgatc tggatcagta tcgctatcaa cctgattttc aggatctgcc tgcgcaagcg
     6481 gcagatcgtg aagtgcagat ctatcgtcgc gctaacggcg atgtcggggt gcgtaacgag
     6541 ctgtggatcc tgccaaccgt gggctgtgtc aacggcatcg cgcggcaaat ccagaaccgt
     6601 ttcctgaaag agaccaacaa cgccgaaggt accgacggcg tgttcctctt cagccacacc
     6661 tacggctgct cacaactggg cgacgatcac atcaacaccc gcaccatgct gcaaaacatg
     6721 gtgcgccacc cgaacgcggg cgcagtgctg gtgattggtc tgggctgtga aaacaaccag
     6781 gttgccgcat tccgtgaaac gttgggcgat atcgatcctg aacgcgttca tttcatgatc
     6841 tgccaacagc aggatgatga gatcgaagcc gggatcgagc atttgcatca gctgtataac
     6901 gtgatgcgca acgataaacg cgagccaggc aaactcagcg aactgaagtt tggtctggag
     6961 tgcggtggtt ctgacggtct ttctggtatt actgctaacc cgatgctggg gcgtttctct
     7021 gactacgtga ttgctaacgg cggtactacc gtactgaccg aagtgccgga gatgtttggc
     7081 gcagagcagt tgctgatgga ccattgccgc gacgaagcaa cgtttgaaaa actggtcacc
     7141 atggtcaacg actttaaaca gtactttatt gcccatgatc agccgatcta cgaaaaccca
     7201 tcaccgggga acaaagcggg cggtatcacc acgctggaag acaaatcact tggctgtacc
     7261 cagaaagcgg gttccagtgt cgtggttgac gtgctgcgtt acggcgagcg tctgaaaacg
     7321 cctgggctga acttgttaag tgcgccgggt aacgatgccg tagcgaccag cgccctggcg
     7381 ggtgcgggct gccatatggt gctgttcagt actggtcgcg gcacgccata cggtggattt
     7441 gtgccgacgg tgaaaatcgc caccaacagt gaactggcgg cgaagaaaaa acactggatc
     7501 gactttgacg cgggtcagct gatccacggt aaagcgatgc cgcagttgct ggaagaattt
     7561 atcgacacca tcgttgagtt tgccaacggt aagcaaactt gcaacgagcg taacgacttc
     7621 cgcgaactgg cgatcttcaa aagcggcgta acgctataaa gtcgaaatga gttgttaata
     7681 aaaaacggcg tttcataatg tgaagcgccg tttgcttttc catacctgat aaggatttat
     7741 catgaccgcg tattggctgg cccagggcgt gggtgtcatc gcctttctga ttggtatcac
     7801 aacatttttc aatcgtgacg aacgtcgctt caaaaagcag ctttcggtct atagcgccgt
     7861 tattggcgta catttttttc ttctgggcac ctatcccgct ggtgccagtg ccatccttaa
     7921 tgccattcgt acattgatta ccttacgcac gcgcagctta tgggtaatgg cgatttttat
     7981 tgtgctgact ggcggaattg gcctcgcgaa gttccatcat cctgtcgaac tattgccggt
     8041 tatcggtacg attgtcagta cctgggcgct gttccgctgt aaagggctca ccatgcgctg
     8101 cgtgatgtgg ttttcaacgt gttgctgggt gattcacaac ttctgggcgg ggtcgatagg
     8161 cggcacaatg attgagggta gttttctgct tatgaatggc ttgaatatca ttcgtttctg
     8221 gcggatgcag aaaaggggaa ttgatccgtt taaagtagag aaaacccctc ccgccgtaga
     8281 cgaaaggggt taaacaatta attacgcagg gcgctatttg ccagacgatc gtcttccgcc
     8341 tggcaagctg ccgcagtgaa cagcacgtca gttgaagagt tcagcgcggt ttcgcaagag
     8401 tcctgcaata cgccgatgat aaagccgacg gcaaccacct gcatggcgat atcgttcgaa
     8461 ataccgaaca tattacaggc cagcgggatc agcagcagag aaccccccgc cacgccggat
     8521 gcgccacagg cacacagaga agccaccacg ctcaacagca gcgccgtggg cagatcgacc
     8581 gggataccca gcgtattaac cgcagccagc gtcaacacgg taatggtgat tgctgcgccc
     8641 gccatattga tggtggcacc cagcggaata gaaacggaat aggtatcgcg atccagattc
     8701 agcttttcac acagtgccat attcaccgga atgttcgccg cagagctgcg ggtgaagaag
     8761 gcatacacgc cgctttcgcg caggcacagc agcaccagcg ggaacgggtt acgacgaatt
     8821 ttccaccaca ccagcaatgg gttaaccacc agcgccacca gtaacataca gccaaccagc
     8881 acgaccagca gttgcgcgta gccccacagt gtggagaaac cggtggttgc cagggtagaa
     8941 gaaaccagac caaaaatacc aatcggtgcg aagcgaatga ccagtttcac cataaaggtg
     9001 acggcattcg acatatcgtt gaccaggttt ttggtggtct cgttaccgtg acgcagtgcg
     9061 aaaccgaggc caattgccca caccaggatc ccgatgtagt tacctttcag cagcgcgtcg
     9121 atggggttgg aaaccatgct cattaccagc ccgcgcatca cttctacaat gcctgacggt
     9181 ggcgaaatat cacccgcgct actggataag tgcagggtag aagggaaggc gaagctgaaa
     9241 acgactgcgg ccagagcagc agagaaggta cccagcagat agaggaacaa aatagggcgg
     9301 atattggttt tctgcccgtg ctggtggtta gcaatagatg ccatcaccag catcaacacc
     9361 aggatggggg caacggcttt cagtgcgccg acgaacaaag tacctaacag accaacagct
     9421 tccgccgcgg gttttgatat ccatgccaga agaatcccca gaacaaggcc gaccaggatt
     9481 tgttttacca ggctgccatg agccagacgc cggaatagcc ccggtgaacg ttgcgtagtc
     9541 atttttcgat cctttcattg tgttatcgca catccctggt gcgttctgtc gacgcatctt
     9601 tcaggatgta acaaagtgtt tgcaaggtcg agtataagga aagttacagc ttcgggaagc
     9661 gaaaaatgct ggattttaaa gagaagtcat attttgaaac ttaatggtta catttgtttg
     9721 acataaacag agggaaggac tggctactaa ggctgattag ataacatatg ggcaaaacta
     9781 tttggaatat gagaacggtt gcgctccttt aatgtgctat gccatataaa gtcactggcg
     9841 cagggagcgc gcagggggcg gccaatcgcc gccgccccct gcacccccag gctctggcga
     9901 acaaaatcgc cgctgcgcga ttccctcagc ttatcccttg cggctaccgg gtcgggcgca
     9961 agggaacgtc cctgtaagat gcgccctcgc cgcgcatcca tgcgcgtcgc cccggcctcc
    10021 agggaacgct tcggcgattt tgacgccacc aacacccgtg cggtcttttt gtaaagagct
    10081 acaagccgat tacataatta atagctcacc tttgtctgga atgagttatc tctaggaatg
    10141 gcttaatata cagcgaatca ttctccccgc tgcttatcat gccgataatt cacccaggcg
    10201 ttgataataa acgtcatcac cagaatgcca aacaccacgc ccagcgagac ggcgattgga
    10261 atatggtaga agtcgacaat cagcatcttg ataccgataa acaccagaat caccgccagg
    10321 ccatatttga gcatcgagaa acgctctgct acgcccgcca gcaggaaata catcgcacgc
    10381 aggccgagga tcgcaaacag gtttgaggtc agcacaatga acgggtcagt ggtcacggcg
    10441 aagatagccg gaatgctatc cacggcgaaa atcacgtcgc tcaattccac cagaatcagt
    10501 accagcatca gcggtgtggc gtacagcaat ccattcttac gcacaaagaa atgctcgttg
    10561 tcgatggtgt cggtcatgcg caaatgaccg cgtagccagc gcaccagcgg cttgtcgcca
    10621 atgcctgatt catcttcatg ggcgagcgcc atcttcacgc cggtaaacag caggaaggca
    10681 ccgaagatat acaggatcca gtcgaactgc gaaattagcc agctgccagt gaagatcatg
    10741 atggtacgca gaacaatcgc cccgagcacg ccatacacca gcacgcggcg ttgtaatgcc
    10801 gccggaacag agaaatagct gaacaacatc agccagacaa agacgttatc gaccgccagc
    10861 gatttctcaa tcagataacc cgtgagaaag gccagtgcct gtggatcggc aacggcgcga
    10921 ccttcagttt gcaccagata ccaccagaaa gcggcgttaa acagtaacga cagcgtcacc
    10981 cagaccagcg accaggccgc agcctgtttc atggtcatgg catgtgcccc acgacgcccc
    11041 tgcaacaaca ggtcgatagc cagcataatg gcgacaacaa cagcgaatcc gccccatagc
    11101 aacggcgtgc cgacagtatt catagaagtt ccttacacat aaaaaaacgg ccaacgtcgg
    11161 aagacgtcag ccgctgcttt ttatgcatag acctcgcctt ccggcaaggt ctcacttaca
    11221 acaaaaaagg aatacgtctg cgtattcctt tcgggttgcc cggtgaccgg atgtggtttt
    11281 tcacacatcg taatgacgat cgaccggcaa tgaagttact cccctttgca ggtaacaaag
    11341 tatgacagac cattcagcca gtcaatggtc tgttacatac cttttacact gttttacttt
    11401 ggcaattata gttttacgct atctgccgga aaaatcaccc cagtctggcg gcggatctca
    11461 gtcagcagtt tggcggtgat gcgactgacc gccagccctg gatgatccac cagatgctca
    11521 tccaccaggg tagcaaacag ctctgcttcg tagagcatgg tattaatatg ctgcggctgg
    11581 gtgagatcct gcatctggct gccacgcggc acgaagcaca ctttctggca ttcagacagt
    11641 ttttcaatca ccagcgatcc cgcttcgccc tgaatctcgc tttccaggac agaatcactg
    11701 acctttgagt gctgcaacgt aacgctgaaa tcaccgtaat ccatcaccac cacgccgtgg
    11761 gcatctacgc cgcttgccaa caaactggcg gttgcctgca cgcttttcgg ttcaccgaat
    11821 aatgccaccg ccgacgccag gcagtaaaag ccgatatcca taattgaacc gttagagaat
    11881 gccggattaa aggtgttggg attctcacca tccaggtaac gttgataccg cgaggaatac
    11941 tggcaatagt tgaaaaagac tttacgcaat ttgccgactt tcggcagcgc ctggcgcagc
    12001 aaatgaaagt tcggcaggct ggcggtttta aatgcctcaa acagcaccac ctgattttcc
    12061 cgcgcacagg caatggcggc atccacttcc gccagattcg acgccagtgg tttctcgcaa
    12121 atcacatgaa ttttatggct aaggaaaagt tgtgtctggg aaaagtgcag ggcattcggg
    12181 ctggcaatat acaccgcgtc aatggcatcg ctttccgcca tcgcttccag cgaggtaaac
    12241 agatgctcga cagaaaaatc attggcgaag tgctgggcct gttcaaggct gcgggaatat
    12301 acggcggtta acttgtattt accgctctca tgggcggcct cgacgaactg gcgagtgatc
    12361 cagttcgtac caatcacagc gaaacgtatc ataacgcgtg gatactccat aaagggattc
    12421 tgtcgtcaat gtagcacgcc tttttcatct tcgacgtgcg cagatacgca ttattctttc
    12481 gccggacagc catgctcact aaacggccca atcatgaccg atggcccgag atatttcggc
    12541 tggcgatgga ggattttggc gtcaatcacc gcgctgacac gagcgagttt ctcccgcagt
    12601 tgagtataaa aaccgtgctt catattaagg tctggcgcgt taaacccgat agcgtcgatg
    12661 ccgtactgtt ttgccagcca gatggcgcgt tggttatgaa attcttgcga gataatggtg
    12721 atatggtttt ccccaaacac ctttttggca cgcaccactg aatcaagcgt tgagaatccg
    12781 gcatagtcgc agaagatcac tttcgccggt acgcctttag cgatcaaagc ctgctgcata
    12841 cctgaggctt catcgtaatt tttacgtccg ttatcgccac tgactaacag ccatttcact
    12901 tttccgacgt ggtacaacgc tgctgcggta tcaatacgtc gggtaaagta gcgattaccc
    12961 ggcctcgccc ccagcaataa acccacgtta cgcgccggaa ctgcgttgac atcgctccag
    13021 gtcagctgct tgctggcgtt gaccattacg cgatcggcga taaaaatggt cgctcctgcc
    13081 acaagtaaca gtaagcaggt aatctttaga gtccggcgag aaaaacaaat acggagaaga
    13141 aggcggggct gcatggtcgt tatccttaac gaaagggggg aggtcgattt taggcgctaa
    13201 agaaagaaaa gataacgcct gacatcattt tcagattttg tcctgcccaa gcgacaactg
    13261 cgttagccac agccgggtgt cgaactcaag ctggtggtac tgcggctcca tatggcagca
    13321 caactggtaa aacgctttgt tatgctcttt ctccttcagg tgcgccagtt cgtgcaccac
    13381 gatcatgcgt aaaaacggtt ccggcgcgtt gcgaaacaca gtagcgacgc ggatctctgc
    13441 ttttgctttc agcttgccgc cctgcacacg agaaacagcg gtatgtaatc cgagcgcgtt
    13501 tttcagtacg tggatcttat tgtcatacat cactttagtg atcggcgggg cattgcgcag
    13561 aaactgattt tttagatcct gggtatattg ccagagggct ttatcggtgg cgtagtcgtg
    13621 tgttcccgga tagcgttttg ccagcacatc gcccagacgc tgttcgttaa tcagcgttcg
    13681 cacctgggaa agtagctgct cgggataacc ctgaagataa gtaagattgc tcataaccgc
    13741 ccacgaaaaa gagaaaaagg gtatactcac gcaccctttt caggggcaac gtcgaaattt
    13801 tatcattcag gagggccgat gagccactta gacaacggtt tccgttcact gacactacaa
    13861 cgttttccgg cgacggatga cgttaacccg ctacaggcgt gggaagcggc ggatgaatat
    13921 ttgctgcaac agttggacga cacagaaatc cgcggcccgg tgttgatcct gaatgatgcc
    13981 tttggtgcgt taagctgcgc gctggcggaa cataagccgt acagcattgg cgactcatac
    14041 atcagtgaac tggcgacgcg cgagaattta cgcctcaacg ggattgatga gtcgagcgtg
    14101 aagtttctcg acagcaccgc cgactacccg caacagccgg gtgtggtgct gatcaaagtg
    14161 ccgaaaacac tggcattgct ggaacagcaa ctgcgtgcgc tgcgcaaagt ggtcacgtcg
    14221 gatacacgta ttattgccgg tgccaaagcc cgtgacattc acacttccac gctggagctg
    14281 ttcgagaaag tgctcggccc gaccaccact acgctggcat ggaaaaaagc gcgcctgatt
    14341 aattgcactt tcaatgaacc gccgctggcc gatgcgccgc agaccgttag ctggaagctg
    14401 gaaggtactg actggactat ccacaaccat gcgaatgtct tctcccgcac cgggctggat
    14461 attggcgcac gcttctttat gcaacatctg ccagagaatc tcgaaggtga gattgtcgat
    14521 ctcggttgcg gtaatggcgt tattggtctg acgctgcttg ataaaaaccc tcaggcgaaa
    14581 gtggtgtttg tcgatgaatc gccgatggcg gttgcttcca gccgtttgaa cgttgaaacc
    14641 aacatgccag aggcgttaga tcgcagcgag tttatgatca acaacgcgct ctccggcgtg
    14701 gagcctttcc gctttaatgc tgtgctctgc aacccgccgt ttcaccaaca acatgcgctg
    14761 accgataacg tcgcctggga gatgttccac catgcccgcc gctgcctgaa aatcaacggc
    14821 gagctgtata ttgttgccaa ccgtcacctg gattacttcc ataaactgaa gaagattttc
    14881 ggcaactgca ccaccatcgc cacgaataat aaatttgtgg tgctgaaagc agtgaagctg
    14941 gggcgtcgtc ggtaagattg ggagttacct gccggatgcg gcgttaacgc cttatccggc
    15001 ctacgttcag cacgttactc ccaggccgga taagacgcgg caagcgtcgc atccggcaat
    15061 gtctgcccag ttctggaatc gcgcttccat aaaacaaaaa atccctctca ccatcctcta
    15121 cattctcttg tttagcgttt ttctacgttt attcttccgt cacacagata aattcatccg
    15181 ttgccaatct gtcaacggat gttatcatat tgccaatttg tcaacggacg tgatgaagaa
    15241 tgcacctgat aactcaaaaa gcattgaaag atgctgcgga aaaatacccg caacataaaa
    15301 cggagttggt ggctctgggg aacacgattg ctaagggata tttcaaaaaa cctgagtcat
    15361 taaaagcagt attcccatct ctggataact tcaaatatct ggataagcat tatgttttca
    15421 atgttggggg caatgaatta cgtgttgtag caatggtctt ttttgaatcg caaaagtgct
    15481 acatacgtga agttatgacg cataaagaat acgatttctt taccgctgtt catcgtacta
    15541 aggggaaaaa atgattgcga ttgccgacat cttgcaagca ggagaaaagc taactgcggt
    15601 ggcacctttt ctggcgggta ttcagaacga ggaacaatac acccaggcgc tggaactggt
    15661 agatcatctg ctgctcaacg atcctgaaaa ccccttgctg gatctggtgt gtgccaaaat
    15721 aaccgcgtgg gaagaatcag cgcccgaatt tgcggaattt aatgccatgg ctcaagccat
    15781 gcctggcggt atagccgtga ttcgtaccct tatggatcaa tatggtttaa cccttgccga
    15841 tctgccggaa attggcagta aatctatggt gtcacgcgtt ttgagcggga agaggaaatt
    15901 aacgctggaa cacgctaaaa aattggcaac gcgattcggc atttctcccg ccttgtttat
    15961 tgattaacgc gacgtgccgg atgcgttgca tccggcgctt aaaataacga tttaaatctc
    16021 cagcgccagc cgtgttccct gggcaattgc ccgtcgtgcg tccagctcca tagccacatc
    16081 gcagccgcca attaaatgca cagttttccc gctatcaatc agcggttgcg ccagcgcgcg
    16141 gtttggctct tgccctgcgc agatcaccac attgtccact gctaatacct gcgtttcgcc
    16201 gttgatcacc acatgcagcc cgtcatcgtc aatcttctga taacttacgc ctgggatcat
    16261 tttcacaccc cgcgagagca gggtggtgcg atggatccag ccggtggttt tgcctaaccc
    16321 ctgtcccggt ttgctggctt tgcgctggag catcacaatt tgccgtgggc tacgcgggat
    16381 ctgcattccc tgcgggctta agccaccagc ctgttgtagg ctgctgtcga tcccccactc
    16441 attacagaac ctggcgatat tctggctggt ggattcgccc ggctgactta aatacatcgc
    16501 cgtatcaaaa ccaatcccgc cacaaccgat gatggcaacc ttgttgccta ccggcgcttt
    16561 gtcgcgcagt acatcgagat aactcaatac cttcggatga tcgatcccgt cgataggcgg
    16621 aacgcgcggc acgatcccac tggcgaggat cgtttcatcg aacgcctgta actgatccgc
    16681 cgtcacggtg tgattgagtt tcagcgtcac gcccgtcact tcgatcatcc ggtggtaata
    16741 gcgcagcgtt tcgtaaaact cctctttgcc ggggatctgt ttggcgatat taaactgccc
    16801 gccgatctcg ctgtgggcat caaacaatgt tacctgatgc ccacgcgccg ccgcgttaat
    16861 ggcaaacgcc agcccagcag gtcccgcacc gaccaccgcc agattttttt tctgcacggc
    16921 aggaaggatt ggcattttgg tttcgtggca ggcgcgagga ttcaccaggc acgaggtgac
    16981 tttgccaacg aagatctgat cgaggcaggc ctgattgcag ccaatgcagg tgttgatctc
    17041 atcggctcgc cccgattgcg cttttgacag cagttccgca tcagcaagaa acggtcgtgc
    17101 catcgatacc atatcggcat cgccgcgcga gagaatatcg tcggcaacct gcggatcgtt
    17161 aatccggttg gtggttacca gcggcagcga gacgtggcct ttcagtttgc gcgtgaccca
    17221 gctaaatgcg ccgcgcggca cgggcgtggc aatggtcgga atacgtgctt catgccagcc
    17281 aatgccggtg ttgataatgg tcgcgcccgc cgcttcaatg gcctgcgcca gctctaccgt
    17341 ttcggcaaaa gtcccgccgt cttcgaccag gtcgagcatc gacagtcggt agataataat
    17401 gaagtcgttg ccgacgcgtt cgcgcaccgc acgcactact tctacggcaa atcgcatccg
    17461 gttgcggtaa tcgccgcccc actggtcact acgctgattg gtgcgcagcg tcagaaattc
    17521 gttgatcaaa tacccttcgg accccattac ctctacaccg tcgtatcctg cctcccgtgc
    17581 cagttgcgcg cagtgggcga aatcgtcgat cagttgcagg atctcttcat gggtgagttc
    17641 gtgaggaacg aaacggttga tgggggcctg caatgcggag ggagcgacca gatgcggttg
    17701 gtagctgtag cgcccggtat gcaaaatttg cagggctatt ttgccgcctt cctgatgtac
    17761 cgcttcggta atggtgcgat ggtgtgggat ctggctggcg tcgttgagca ttgcgccacc
    17821 ttccatgcca acgcctgtta aatctggtgc aatgccgccg ctgacaatca gcgccacgcc
    17881 gtgacgggcg cgttcggcat aaaacgctgc cagccgctcg gcaccgtccg ggtattcctc
    17941 cagcccggtg tgcattgagc ccatcaacac gcggtttttt aacgtggtaa aacctaaatc
    18001 cagcggggcg aacagcgacg ggtagctcat aaaattgtcc agtatgtaaa ataattgtta
    18061 tgtggtcgga tgagttctaa tttagccgtc gatggagaaa agggaaaagg ggagtgcggg
    18121 gtttgtgatg ggattcaaaa agaatacgcc cggtggtaac gccggatgca acgcttgccg
    18181 cgttttatcc ggcctggggg attgcacatg tagggcggat aaggcgttca cgccgcatcc
    18241 gccagccgtt gcaaattact gcttacggaa aaaatcgtta tacaacatat acaaatgagc
    18301 ggcactccag gagaaatttg gtgcgccttg ctgtgcgcca gtcagcgggt tgtaattctc
    18361 ctgaattggg ccatcggcgg ttaatccttt ggcgtgccgg aagaacgtat ccgccagctt
    18421 cagggcatca tcacgataac cgtaacgctc catccctttc agaccaaacc agaactgatc
    18481 cacccatacg cgcccacgcc agtagatatc agcgccaaaa gccggattgg ttaacgccgc
    18541 cgttcccagc gggacaaagg tgttgaactc tttaggatcg agcatcacct tcaccaccgc
    18601 gtcggcgttg gcctgcgttg ccgcaccgtt aaacagcggc gaccagcctt ccggcccttt
    18661 accgcgctca acaatcggtt tgcccgcgca gccgttcgcc agtggtttat cttcaatacg
    18721 tacgtcatag tagaactggg tagtcgggtc gaacatacag gtgttgatgt agtccgcgag
    18781 ctgctgtgcc aactggcgat agcgtttggc ctcttctggc ttaccgagga tggttgccat
    18841 ctccgccaga taatggttat cgctgtacat atagctggcc tgatccaccg actcctgcaa
    18901 tagcgagtag cccagcaacg ttccgtcctg actgcggttt tcggcgaatt tcaccgtcca
    18961 gtcgctacgt ttgccgccat tagcgacata tttatccagc tgttctttgt cgataaaccc
    19021 aaagacggcg gcatcatcac gccccgattc ccacgatgca gcaacctgtg ccggaatttc
    19081 cagactgtcg tacaggcctt tctccaccac gcgggcgtag ttgttcagcc cagactgcgt
    19141 ctcttcttta tcgccttttt tcaccgtaaa cagcatctcg ccgctctcag tgttgtgggc
    19201 tttgtcgcgg gtcgcgccat attccggcac gccgttgccg ttgtgatcgc ggttacgtaa
    19261 ccaccagtcg tgataggcca ccagtttcgg gtacatctct gccaaccagg ctttatcctg
    19321 ggtgacgttg tacacttcca tcaccgacca ggcggcaagg ctgggtttgg tattacgttc
    19381 gttccagttg ccgccatcgc caccacgctc ggggctaagg ttccacgcta tcaggtcggg
    19441 gacaaagccc acatcctgcg gacgcacgct atcgccaggc tggatctgcc aggagaagac
    19501 cgcgcggata ttctctttgg cgatgtccgg attgaaatgc gccatcgcaa acgcctgctt
    19561 ccaggtatcc cacggccagg tctgattgcc ggagaaccag cgcccggtca ccgacggtgt
    19621 aacggtgtta aatttcaccg caccgcccgg tgagagccag ttaccgttga gcgtttcgat
    19681 cgctttcacc gcgacgcgcg tctgttccgg cgtcgcatcc ggattggtta accctttctt
    19741 caggtattct tcccagcgtt gctgcgaggc ggtgagataa aacgccggac gcgccagaat
    19801 atcgcggatc tgcatttgct ctttgctaac ttcctgagcg gtcagcagat gggaataggt
    19861 ggtgtagagc gtggtcgaac cgttgatatg cgccttactg gtaaagcgat taccgttgat
    19921 ttcagtctgc accggcaggg atttatgcac ctgatattcc gattcgccgg aggtcagcag
    19981 atcccaggtg gcgcgcactt tgccaaaggt gactttcagg ccatcacggg tggcgctgat
    20041 tttgcgctga tagtcagggt attcgccagc aatggtttta tcggaaagcg gtttcccttc
    20101 tttcgcttcc agtttttcca gcagttcgcc gtcccacacc agatccagcg gtttattgct
    20161 ggtgattttg gtttccagta gtgacgtgcg cggcgtggcg aagcgcagag tcatttcgac
    20221 ttgcacatct tttgctgtca gtttttgcac cagcgcaccg ggaatactgt atgcctccag
    20281 cgtaaagtcg actttcttgc cgtcctgcca gacggtcagg cggtcgaaat tgctggccat
    20341 aaagttgatg tactcttccg tcagcagcgc aacgcccgga aagccgccca tggtgttagg
    20401 gccgtctggc aacagatgac catgccaggc tccgagatcg aaaaacggat taaaacgctg
    20461 gtgatcgtcg taatcgtaat ccttcatgta ctgcggcgcg ccagtacggt taatcacgtt
    20521 tttataattg tcggcgttag cggcatgggc ggaaaaactt atcagcagag cgcaggttac
    20581 tggcgttaaa atagttttta ttttcatgaa attacagtcc ttattaccag tagaaatatt
    20641 gcatcaggaa gatttggtcg ctgggatcgt gagaggcatt tttcatgtag aagcggctat
    20701 caaaagccgt ggcgaaacgt ccgccgccgt attcgtacca gataccaaac tgcatgaaag
    20761 aggtggtttc atcgtcagcg tggtcagggc gatgtttggc gtaggtgtaa gcggtggaga
    20821 gataaacgcc ctttaacgct tcatacatgg cgctcaccat catcccactt tcacttcccg
    20881 gtacgtccag cccgtcggcg cgtccggtat ggtgccaggc gcgagcggca aagttaggag
    20941 ttagcaggcc cgtgagatag agctgtcctg tgccgtcggt gatttccaga ccgttcatcc
    21001 aggtgatgcc ttcggcaagg tcatactgga tgtaaccgtt catcattgcc ggataggtgt
    21061 atttgtcgtc atagcgatcg tatttgccaa aatgtagcca ggctttgctt tcatcatgat
    21121 gtccggcagg agtcgcggtg acgctataac gaaagttgcc gcttaagttc tgtaccttca
    21181 gcgcagtcat aatgtcgcgg gtattgggaa tgacgtagcc cagatcgggg gtgaaatcgc
    21241 cccaccattg caggtcatcc agcgaggaat cctgacgcag actgagcatg atttcggtgc
    21301 catcattggt acgataaccg ccatagaaac ggttaattcc gccctcgaaa ccaccccagc
    21361 tatggtctgg cacccaggca ttgccgtcgt ggtcggcctg cacggtccag ccttcgccgt
    21421 aaatcaggcc gaaccaggag ccgtacttca tctccaggcc accttcaata taggtaccgt
    21481 cgctgtattt atgtttattc tcaccctcta aatccatata tccaccaccc cctaatccct
    21541 aattcgccgt aaaaacgaaa tgaaggagaa gatgtcgagg taattgcggg tgtttttgtg
    21601 tcgtcatgtt ctgccggacg tgcttcttct cctgccgcag aaaaggcgta gcaaaacggc
    21661 agagcaagta atgctctgca tggtgcagtg attaatttca ttgtgt
//
LOCUS       DAKISA010000077        21049 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.077, whole genome shotgun sequence.
ACCESSION   DAKISA010000077 DAKISA010000000
VERSION     DAKISA010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21049
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.077"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(12..557)
                     /gene="hemG"
                     /locus_tag="OVW45_002885"
     CDS             complement(12..557)
                     /gene="hemG"
                     /locus_tag="OVW45_002885"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCV5527286.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAQLTNKPTLK"
     gene            complement(569..2020)
                     /gene="trkH"
                     /locus_tag="OVW45_002886"
     CDS             complement(569..2020)
                     /gene="trkH"
                     /locus_tag="OVW45_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCV5527287.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            complement(2059..2673)
                     /locus_tag="OVW45_002887"
     CDS             complement(2059..2673)
                     /locus_tag="OVW45_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCV5527288.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            complement(2673..4004)
                     /gene="pepQ"
                     /locus_tag="OVW45_002888"
     CDS             complement(2673..4004)
                     /gene="pepQ"
                     /locus_tag="OVW45_002888"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCV5527289.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHASVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            4194..6383
                     /gene="fadB"
                     /locus_tag="OVW45_002889"
     CDS             4194..6383
                     /gene="fadB"
                     /locus_tag="OVW45_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCV5527290.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            6393..7469
                     /gene="fadA"
                     /locus_tag="OVW45_002890"
     CDS             6393..7469
                     /gene="fadA"
                     /locus_tag="OVW45_002890"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCV5527291.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LLAEVPHSVPAVTVNRLCGSSMQALHDAARMIMTGDAQACLVGGVEHMGHVPMSHGVD
                     FHPGLSRNVAKAAGMMGLTAEMLARMHGISREMQDAFAARSHARAWAATQSGAFKNEI
                     IPTGGHDADGVLKQFNYDEVIRPETTVEALATLRPAFDPVSGTVTAGTSSALSDGAAA
                     MLVMSESRARELGLKPRARVRSMAVVGCDPSIMGYGPVPASKLALKKAGLSASDIGVF
                     EMNEAFAAQILPCIKDLGLMEQIDEKINLNGGAIALGHPLGCSGARISTTLLNLMEHK
                     DVQFGLATMCIGLGQGIATVFERV"
     gene            complement(7659..8360)
                     /gene="fre"
                     /locus_tag="OVW45_002891"
     CDS             complement(7659..8360)
                     /gene="fre"
                     /locus_tag="OVW45_002891"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCV5527292.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(8407..9900)
                     /gene="ubiD"
                     /locus_tag="OVW45_002892"
     CDS             complement(8407..9900)
                     /gene="ubiD"
                     /locus_tag="OVW45_002892"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCV5527293.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            10067..10555
                     /gene="rfaH"
                     /locus_tag="OVW45_002893"
     CDS             10067..10555
                     /gene="rfaH"
                     /locus_tag="OVW45_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCV5527294.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASLAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            complement(10552..11334)
                     /gene="tatD"
                     /locus_tag="OVW45_002894"
     CDS             complement(10552..11334)
                     /gene="tatD"
                     /locus_tag="OVW45_002894"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCV5527295.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTRFGIAF"
     gene            complement(11376..12152)
                     /gene="tatC"
                     /locus_tag="OVW45_002895"
     CDS             complement(11376..12152)
                     /gene="tatC"
                     /locus_tag="OVW45_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCV5527296.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            complement(12155..12670)
                     /gene="tatB"
                     /locus_tag="OVW45_002896"
     CDS             complement(12155..12670)
                     /gene="tatB"
                     /locus_tag="OVW45_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCV5527297.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            complement(12674..12943)
                     /gene="tatA"
                     /locus_tag="OVW45_002897"
     CDS             complement(12674..12943)
                     /gene="tatA"
                     /locus_tag="OVW45_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCV5527298.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            complement(13022..14662)
                     /gene="ubiB"
                     /locus_tag="OVW45_002898"
     CDS             complement(13022..14662)
                     /gene="ubiB"
                     /locus_tag="OVW45_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCV5527299.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            complement(14659..15264)
                     /gene="ubiJ"
                     /locus_tag="OVW45_002899"
     CDS             complement(14659..15264)
                     /gene="ubiJ"
                     /locus_tag="OVW45_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCV5527300.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            complement(15278..16033)
                     /gene="ubiE"
                     /locus_tag="OVW45_002900"
     CDS             complement(15278..16033)
                     /gene="ubiE"
                     /locus_tag="OVW45_002900"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCV5527301.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            complement(16128..17555)
                     /gene="rmuC"
                     /locus_tag="OVW45_002901"
     CDS             complement(16128..17555)
                     /gene="rmuC"
                     /locus_tag="OVW45_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCV5527302.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LRGGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQNEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            complement(17696..18457)
                     /gene="udp"
                     /locus_tag="OVW45_002902"
     CDS             complement(17696..18457)
                     /gene="udp"
                     /locus_tag="OVW45_002902"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCV5527303.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGIMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            18719..19531
                     /locus_tag="OVW45_002903"
                     /pseudo
     CDS             18719..19531
                     /locus_tag="OVW45_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
     gene            19925..20986
                     /gene="hcp"
                     /locus_tag="OVW45_002904"
                     /pseudo
     CDS             19925..20986
                     /gene="hcp"
                     /locus_tag="OVW45_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278761.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
BASE COUNT         5183 a         5649 c         5266 g         4951 t
ORIGIN      
        1 attctttatg cttatttcag cgtcggtttg ttggttaact gggcgatttc tcgggcgaaa
       61 ttcgccacct gctcccaatc ggtatagaca acttctttgc gcgtatccgt ttcaccgcct
      121 gacatcttca taattagctt gatcataaaa cgatcgtacc agcgataacg tgggtaacgc
      181 agcgccccgg caatgaccgc gcagcgatcg ggacgccatt gcgagttcat cagaaacttc
      241 cgcgcgtagc tgttggtctg tggagtacgc ttctccggtt tgcgcgccac caggtttacg
      301 gaataaaagg cgctcggcat cgaattcagt cgcgtcgcat gttttttgac aaattcctgg
      361 aacgcagagt ggtagtgacc atagcgaata gaagcaccaa tgaccacacg atcataattt
      421 tcccattgtg gttcttcaat gcggtgcaca ttggcgacat ccgtctggat ccccagttct
      481 ttcagttccg aagccaggta ggaggcaatc tcgcgcgttt gtccgtccct tgttgagaaa
      541 agaattaatg ttttcacgta ttactccatc attcacgcca gaaagtcggg gtaaagagca
      601 ccagcaatgt aaagacctcg agacgaccaa acagcatgtt ggcaatcagg atccatttag
      661 ccaccgggtt catactggta aagttatcag caaccacgcc aagccctggc cccaggttat
      721 tcaatgtcgc aacaactgag gcaaaggcag aaaagtcatc cacgcccgtg gcgataatcg
      781 ccagcatact gacaataaac accaatgcat aggcggagaa aaatccccaa acggcttcga
      841 ggatacgttc cggcagtgcg cgattcccca gcttaatgct atagacggcg ttcggatgca
      901 ccagtcgttt cagctcacgg ttcccctgct taaacagcag caggatgcgg atcactttca
      961 ggccaccgcc cgttgacccg gcgcaaccgc cgataaaagc tgaacataaa agcagtaccg
     1021 gcaaaaagag cggccagcgg gcaatgctgt cagtcgtaaa cccggcggtt gtcgccatcg
     1081 ataccacctg gaaaaacgcc tggttaattg tcatcagcgc cgaactgtag acattatgaa
     1141 accacagtac gagtgtacaa ataaccacca gcgtaaactg tacgccgata aacatgcgaa
     1201 attccggatc gcgccaataa accttcagac tacgcccact taacagtgaa aagtgcagac
     1261 cgtagttaca gccggagatc agcaggaaga tagcaatgat agtgttaata gtcgggctgt
     1321 cgaaataacc gatactggca tcatgtgtcg agaagccgcc aatagcgata gtcgcaaagc
     1381 tatggccgat ggcatcaaag gcatccattc cggcaaacca caacgccagc gcacaggcga
     1441 cggtcagcaa gacataaatc aaccacaggg ttttcgccgt ttccgcaatt cgcgggcgca
     1501 ttttgttatc tttcagcggg ccgggcattt ctgcgcgata gagctgcatc ccacccacgc
     1561 cgaggatagg cagtatcgca accgctaaca cgatgatccc catcccgcca aaccattgca
     1621 gcatctggcg ataaaagagg atggcatgag ggagcgaatc cagccccacc agcgtagtgg
     1681 ccccggtggt ggtcaggcca gagaaagatt caaaaaacgc atcggtaatc gtgaggttcg
     1741 ggctttccga gaagataaaa gggagcgcac cgacactacc cagcacggtc cagaacagca
     1801 ccactatcag aaacccctca cgggatttca gttcgccctt ctctttacgg ttcggccacc
     1861 acagcataga accaatggcg agggcgacaa aaaaggtctg ggtaaaagcg cggcccgctc
     1921 catcccggta gatgagtgct accagcccag ggataatcat ggtccctgaa aataagatga
     1981 ccagtagtcc aacgattcgg gtaatggcgc gaaaatgcat ctctgccgct tccttagttc
     2041 tgcaaaacga agtggggatt attcttcaat cgctaacaat tgcaatgaac cacggctaaa
     2101 atccgccagc tttgcggaaa attcagccac tttcgccgcc ggaagcgcca cccgcagcag
     2161 aacgaatgcc tgataatcac tgttgataat tttgccgtca cactgcccca gcaacgcttc
     2221 aatgccggtt aactgatgat attcacattg caaagtatat tcggttaatg gcgtcttgcg
     2281 ttgggtcgtt agctggcgca gcgcctgatt cacgccgccg ccatacgctt tcactaaccc
     2341 accggtgcca agcaatatgc cgccgtagta gcgcactacc acagcggtaa tttccccgac
     2401 gccgctgccc attagctggg cgagcatcgg tttacctgcc gttcctgccg gctccccgtc
     2461 gtcagagaaa cccagctgtt gagaatcatc cggcgcaccc gccacccacg ccacgcaatg
     2521 gtggcgggca tcggggtgtt ctgcccgcac cgattcaaca aacgctttcg ccgcctcaac
     2581 gccatcggta tgcgccaaca tcgttatgaa gcggcttttt ttgatctctt caacgaccgt
     2641 gaccggtgcc gcaggaatta accagctttc catcacgcca gtttcagatc ccgggtcatg
     2701 ttttccacgt tgttttcgtg gatcaccacg ttgtcttcga tacgaatgcc gccgaacggt
     2761 ttcagtgctt caattttctg ccagttgaag tgcttgctga actgcccttc acgccacggt
     2821 gccagtagcg attcaatgaa gtagataccc ggttcgatgg ttaacaccat acccggctgg
     2881 agaatacggg tgcagcgcag gtacggatat tttgccggtg ccgcgaggtg tgtaccgcta
     2941 tcatcctgca taaaaccggc tacgtcatgc acctgcaggc ccagcggatg accgatacca
     3001 tgcggcataa acggtccggt gagatcgttt tcgaccatcg cttcttcact catatcggtg
     3061 atgatttgat gtttacgcag cagtttggcg atgcgctgat ggaactggat gtggtaatcc
     3121 acatagctaa cgcctgcttt catggtggcg atcagcgcca gttgttcatc atttacgtct
     3181 ttcaccagct gtgcgtagtc gttgtcgctt tttgccgacc aggtacgagt caggtcagcc
     3241 gcatagccgt tatattcggc cccggcatcc agcaggaagc tgcgcatctc ttccggtgcc
     3301 tgatgatcca gtttggtgta atgcagcacc gaagcgtgtt cgttaagcgc cacaatgttg
     3361 ctgtaaggta cgtcggtatc acgatgaccg gtcgcggtca gataggcgat gttgatatcg
     3421 aactcgctca tgccagaacg gaacgcttct tctgccgcac gatgaccgtt gaccgccatt
     3481 ttctgcgctt cacgcataca ggccagttcg tactcggttt tgaaggagcg gtagtaatgc
     3541 aggtagtcga tcaccccttt cgggttgatg ttgctggcct caatacccag ttgcagcgca
     3601 cgttccggca ccggaccgat ataaccgata ttgccgcgcg cagcgggcag caggctacca
     3661 atgccatcgg ctttcggcag tgcgatcact tccacatctt cagtccagaa ggaggtcggc
     3721 agcggttcga cgttgtgcca gtaatcaacc ggcagataga accacagttt cggcttgttc
     3781 acgccatcca ccagcaacca gcagtttggc acctgagtta ccggcaccca cgctttgaat
     3841 tgcgggttca ctttaaacgg atagggatga tcgtcgagaa aaacgttgaa cagctcgccg
     3901 gagtgaataa gtaatgcatc cagcttgaag cgcgccagcg catcgcgagt ccgttcttgt
     3961 aaggtagcta tatgattttt atagagcgag gccagtgatt ccatttttta cccttctgtt
     4021 tttttgacct taagtctccg catcttagca catcgttcat ccagagcgtg atttctgccg
     4081 agcgtgatca gatcggcatt tctttaatct tttatttgca tatttttaac acaaaataca
     4141 cacttcgatg catctggtac gaccagatca ccttgcggat tcaggagact gacatgcttt
     4201 acaaaggcga caccctgtac cttgactggc tggaagatgg cattgccgaa ctggtatttg
     4261 atgccccagg ttcagttaat aaactcgaca ctgcgaccgt cgccagcctc ggcgaggcca
     4321 tcggcgtgct ggaacagcaa tcagatctaa aagggctgct gctgcgttcg aacaaagcag
     4381 cctttatcgt cggtgctgat atcaccgaat ttttgtccct gttcctcgtt cctgaagaac
     4441 agttaagtca gtggctgcac tttgccaata gcgtgtttaa tcgcctggaa gatctgccgg
     4501 tgccgaccat tgctgccgtc aacggctatg cgctgggtgg tggctgcgaa tgcgtgctgg
     4561 cgaccgatta tcgtctggcg acgccggatc tgcgcatcgg tctgccggaa accaaactgg
     4621 gcatcatgcc gggctttggc ggttctgtac gtatgccacg tatgctgggc gctgacagtg
     4681 cgctggaaat cattgccgcc ggtaaagatg tcggcgcgga tcaggcgctg aaaatcggtc
     4741 tggtggatgg ggtagtcaaa gcagaaaaac tggttgaagg cgcaaaggcg gttttacgcc
     4801 aggccattaa cggcgacctc gactggaaag cgaaacgcca gccgaagctg gaaccgctaa
     4861 aactgagcaa gattgaagcc accatgagct tcaccatcgc taaaggaatg gtcgcgcaaa
     4921 cggcggggaa acattatccg gcccccatca ccgcagtaaa aaccattgaa gctgcggccc
     4981 gttttggtcg tgaagaagcc ttaaatctgg aaaacaaaag ttttgtcccg ctggcgcata
     5041 ccaacgaagc tcgcgcactg gtcggcattt tccttaacga tcaatatgta aaaggcaaag
     5101 cgaagaaact caccaaagac gttgaaaccc cgaaacaggc tgctgtgctg ggcgcaggca
     5161 tcatgggtgg cggtatcgct taccagtccg cgtggaaagg cgtgccggtt gtcatgaaag
     5221 atatcaacga caagtcgtta accctcggca tgaccgaagc cgcgaaactg ctgaacaagc
     5281 agcttgagcg cggcaagatc gatggtctga aactggctgg cgtgatctcc acgatccacc
     5341 caacgctcga ctacgccgga tttgaccgcg tggatattgt ggtagaagcg gttgttgaaa
     5401 acccgaaagt gaaaaaagcc gtactggcag aaaccgaaca aaaagtacgc caggataccg
     5461 tgctggcgtc taacacttca accattccta tcagcgaact ggccaacgcg ctggaacgcc
     5521 cggaaaactt ctgcgggatg cacttcttta acccggtcca ccgaatgccg ttggtagaaa
     5581 ttattcgcgg cgagaaaagc tccgacgaaa ccatcgcgaa agttgtcgcc tgggcgagca
     5641 agatgggcaa gacgccgatt gtggttaacg actgccccgg cttctttgtt aaccgcgtgc
     5701 tgttcccgta tttcgccggt ttcagccagc tgctgcgcga cggcgcggat ttccgcaaga
     5761 tcgacaaagt gatggaaaaa cagtttggct ggccgatggg cccggcatat ctgctggacg
     5821 ttgtgggcat tgataccgcg catcacgctc aggctgtcat ggcagcaggc ttcccgcagc
     5881 ggatgcaaaa agattaccgc gatgccatcg acgcgctgtt tgatgccaac cgctttggtc
     5941 agaagaacgg cctcggtttc tggcgttata aagaagacag caaaggtaag ccgaagaaag
     6001 aagaagacgc cgccgttgaa gacctgctgg cagaagtgag ccagccgaag cgcgatttca
     6061 gcgaagaaga gattatcgcc cgcatgatga tcccgatggt caacgaagtg gtgcgctgtc
     6121 tggaagaagg cattatcgcc actccggcgg aagcggatat ggcgctggtc tacggcctgg
     6181 gcttccctcc gttccacggc ggcgcgttcc gctggctgga caccctcggt agcgcaaaat
     6241 acctcgatat ggcacagcaa tatcagcacc tcggcccgct gtatgaagtg ccggaaggtc
     6301 tgcgtaataa agcgcgtcat aacgaaccgt actatcctcc ggttgagcca gcccgtccgg
     6361 ttggcgacct gaaaacggct taaggagtca caatggaaca ggttgtcatt gtcgatgcaa
     6421 ttcgcacccc gatgggccgt tcgaagggcg gtgcttttcg taacgtgcgt gcagaagatc
     6481 tctccgctca tttaatgcgt agcctgctgg cgcgtaaccc ggcgctgctg gcagaagtgc
     6541 cgcactctgt cccggcggtt accgtcaatc gcttgtgtgg ttcatccatg caggcactgc
     6601 atgacgcagc acgaatgatc atgactggcg atgcgcaggc atgtctggtt ggcggcgtgg
     6661 agcatatggg ccatgtgccg atgagtcacg gcgtcgattt tcaccccggc ctgagccgca
     6721 atgtcgccaa agcggcgggc atgatgggct taacggcaga aatgctggcg cgtatgcacg
     6781 gtatcagccg tgaaatgcag gatgcctttg ccgcgcggtc acacgcccgc gcctgggccg
     6841 ccacgcagtc gggcgcattt aaaaatgaaa tcatcccgac cggtggtcac gatgccgacg
     6901 gcgtcctgaa gcagtttaat tacgacgaag tgattcgccc ggaaaccacc gtggaagccc
     6961 tcgccacgct gcgtccggcg tttgatccag taagcggtac ggtaacggcg ggtacatctt
     7021 ctgcactttc tgatggtgcc gctgccatgc tggtgatgag tgaaagccgc gcccgtgaat
     7081 taggtcttaa gccgcgcgcc cgtgtgcgtt cgatggcggt cgttggttgt gatccgtcga
     7141 ttatgggtta cggcccggtt cctgcctcga agctggcgct gaaaaaagcg gggctttctg
     7201 ccagcgatat cggcgtgttt gagatgaacg aagcctttgc cgcgcagatc ctgccgtgca
     7261 ttaaagatct cgggctaatg gagcagattg acgagaagat caacctcaat ggtggtgcga
     7321 tcgcgctggg tcatccgctg ggttgttccg gtgcgcgtat cagcaccacg ttgctgaatc
     7381 tgatggaaca caaagacgtt cagtttggtc tggcgacgat gtgtatcggt ctgggtcagg
     7441 gtattgcgac ggtgtttgag cgggtttaac cacttgccgg atgcggcgtg aacgccttat
     7501 ccggtctacc gatcctgcac caatgtaggc ctgataagac gcgccagcgt cgcatcaggc
     7561 atcgtgcacc gattgccgga tgcgacatag atctaaatgc cactgaataa agttttagtt
     7621 gccgttcttc ccgcctgtca ggggcgggtt tttatatctc agataaatgc aaacgcatcg
     7681 ccaaacagac gatcttcccg cgcattacgc tcactgcaaa acaggtcacg agcaattttc
     7741 gccatctcaa aacgtccggc aatatagata tcatgctctg ccagcgtacc atgatcctgc
     7801 aagaccgccg ttaacacggt gccagtacgc ccacgccagc ccgcttccgg ttgttcaacc
     7861 accggcacca cttgcagacc aggatgcttc agcgaaagcg cctcaagctc gcagagatca
     7921 tacagatgtt gctcttcacg cccaccccag taaatggtga tatcgcgatt gggattacgc
     7981 gccaacgccg tcaataaaat cgagcgcgca taggagaacc cggtgccgcc cgcaatcaaa
     8041 atcatcggac gctcttcatc atcgcgcagc cacgcttctc cgtggggaat atcgaccacg
     8101 atttgatgat ctttgaggat gcggtccatg actgctttcg cgtaaaggtt gatttcagaa
     8161 gcgccaatat gcagctcgat aaaccctttt tcatccggcg tcgaagccat tgaaaacgga
     8221 cgtttgtcgc gctcatccat cactaccatc aaatactgac cagcacgaaa agaaaaggcc
     8281 gcgtctggta cgattcggac acggtataca gtatccgtga tagcttctac cgaggtcact
     8341 ttacagctta aggttgtcat gcgctttctc tgtcggatca aataaatagg gcaaaactaa
     8401 cgcgcatcag gcgcttttac cgttgttaaa aatagccagt tcatcccaga tggcgtcaat
     8461 atgcgcgacg acatcaggat cttttttgat gggacgtccc cattcacgct gggtttcccc
     8521 cggccattta ttcgtggcat ccagccccat ttttgaaccc agcccggaga caggcgaggc
     8581 aaaatccaga taatcaatag gcgtattttc taccagaaca gtatcccgcg ccgggtccat
     8641 acgggtggta atcgcccaaa tcacatcgtt ccagtcgcgt gcgttgacgt catcatcgca
     8701 aacgatcaca aatttagtgt acataaactg gcgtaagaac gaccagacgc ccatcatgac
     8761 gcgcttcgcg tgtccggcgt actgtttttt gattgtcact accgccagac gataagaaca
     8821 gccttccggt ggcaggtaaa aatcgacaat ttccgggaac tgtttttgca gaatcggcac
     8881 aaacacttcg ttcagtgcga cacccagcac cgcgggttca tccggcggac gaccagtata
     8941 ggtggagtgg taaatcgcat cttcacgctg ggtaatatgc gtcacggtaa ataccgggaa
     9001 actatcgact tcgttatagt aaccggtgtg gtcgccatac ggcccttccg gcgcagtttc
     9061 gccttgttcg atataccctt ccagcacaat ctccgcactg gcgggcactt caaggtcatt
     9121 ggagatacac ttcaccactt cggtcttggt accgcgcagc aatccggcaa acgcatactc
     9181 tgaaagtgta tccggaacgg gggtgactgc accgagaatc gtggcgggat cggcacccag
     9241 cgccacagaa accgggaaac gttcgcccgg atgcgccgca caccactcct gataatccag
     9301 cgcgccgccg cgatgcgaca gccagcgcat aatcagtttg tttttaccaa tcagctgctg
     9361 gcgataaatg cccagattct gccgctcttt atgcgggccg cgcgttacgg tcagccccca
     9421 ggtaatcagc ggcgcggcat cttccggcca gcaggtcata atgggaatgc gattgagatc
     9481 gacgtcatcg ccagagacga ttttttgttg gcagggcgca ccacgcagcc gctttgtcgg
     9541 catgttcaat acttgcttaa actgcggcag tttatcaaac aggtcgcgga aaccttttgg
     9601 cggctcgggc tctttcagaa acgccaataa tttaccaact tcacgcagcg ccgaaacatc
     9661 ttcctgcccc atgcccatcg ccacgcgctt tggcgtaccg aacaggttgc acagcaccgg
     9721 cattgagtag cctttagggt tttcgaacaa cagcgcaggc ccaccggcac gcaaagtgcg
     9781 gtcagcaatt tcagtgattt ccagatgcgg atccaccggg agcgtgatac gttttagctc
     9841 accctgctgt tcaagcagcg tcaagaagtc gcgtaaatcg ttatatttca tggcgtccat
     9901 tgtagcctct taatctacgc ccattatacg gcgttcatct ttgcgatgct gtaaatttgt
     9961 taaattagcg tgaactctga cggtataacg caaaccgggg aatataatta acttagcgta
    10021 aagcttttgc tatccttgcg ccccgattaa acggataaga gtcattatgc aatcttggta
    10081 tttactgtac tgcaagcgcg ggcaacttca acgtgcccag gaacaccttg agagacaggc
    10141 tgtgaattgc ctggcaccga tgatcaccct ggaaaaaatc gtgcgtggaa aacgtactgc
    10201 agtcagtgag ccattgtttc ccaactacct gtttgtcgaa tttgatccag aagtgattca
    10261 taccacgact atcaacgcga cccgcggtgt cagccacttc gtgcgctttg gcgcgtcgct
    10321 agcgatagtc ccatcggcgg tgattcatca gctatcggta tataaaccga aagacattgt
    10381 cgatccggca accccttatc cgggagataa ggtgattatt accgaaggcg cgttcgaagg
    10441 ctttcaggcc attttcaccg aacccgatgg tgaggctcgc tccatgctat tgcttaatct
    10501 tattaataaa gagattaagc acagtgtgaa gaacaccgag ttccgcaaac tctaaaacgc
    10561 aatcccaaac cgtgttttga cattagcatc cgtggtggca gccagccatg cggcatcttc
    10621 tccacgccag tgcgcaatac gttgcaaaat atggggcaga tgggctggct cgttgcgccg
    10681 ggatgatggc tttggcgtga gatcgcgagg gagcagatac ggcgcatcag tttcgatcag
    10741 taatttttcc gccggaatca acggcaaaag ttcccgcagc tccagtccgc gtcgttcatc
    10801 gcaaacccaa ccggtaatgc cgatataaat tccatgcgcc acgcacgcct gcatctcttc
    10861 gcgtgtgccg gtaaagcaat gaagaaccgc accaggcagt ttatccagcc acggctccag
    10921 caatgtcata aaccgctcgt gggcatcgcg acagtgcata aataccggca tgtttaaatc
    10981 tgcggcaatg cgtagctggg caacaaaagc gcgttcctgc tcttccggcg tcgaaaagtt
    11041 gcggttaaag tcgagaccac attcaccaat cgccaccact tctggctgcg cggccagctc
    11101 aataatcgct tcttcagtcg cagcttgcca ctggctgctg tcgtgaggat gtacgcccgc
    11161 cgttgaccaa caggacgaat actgacgcgc cagcttttgc gcctgctggc tttcacgcag
    11221 gttagtgccg gtgatgagta gcccattaac tcccgcgtca aaagcgcgcg ctacaacatc
    11281 atcacggtct ttcgcaaatt gcgaactggt caaattaacg ccgatatcaa acatcctgta
    11341 ctccatatga caaccgccct gacgggcggt tgaatttatt cttcagtttt ttcgctttct
    11401 gcttcagcgt cgttttcctc ttcccgattt cgccctttac caacgtaaaa gcgtgagaag
    11461 aagacaccga tttcaaacag acagtacatc gggatcgcca atagcgtttg cgagaagaca
    11521 tccggcggcg tcagcaacat cccgacaacg aatgcaccaa ccagcacata cgggcgtttt
    11581 ttgcgtaagt cttctggcga ggtaatcccc atccagcaca gcagcacaat tgccaccggc
    11641 acttcaaagg agacaccaaa cgccataaac agcgccataa cgaagcttaa atagctggcg
    11701 atgtcggtgg atacctgcac cccttccggc gcggtattgg caaggaagcc aaatgccagc
    11761 ggaaagacca caaagtaggc gaatgccatg ccgatataaa acagcagaga gctggaaacc
    11821 agcagcggca ccaccaggcg acgttcatgc ttatacagcg ctggggcgat aaatgcccac
    11881 acctgataga gaatcaccgg cgctgacaga atcagcgaca ccataaaggt cagcttgatc
    11941 ggcgtaaaga acggcgaggc cacgtcggtg gcgatcatcg ttgaaccttg cggcaactgc
    12001 ttgatcaatg gcgcggatac caggtgatag atgtcattgg cgaaatagac cagacacagg
    12061 aatatcacga tcaccgcgat aatgcagttc agcagacgct tacgcagctc aattagatgc
    12121 gtgataagcg gttgagtatc ttctacagac atgtttacgg tttatcactc gacgaagggg
    12181 aaggtgcagc ggttttcggt tcagcgtccg cagcaggttt taccaccggc tctggcgtgg
    12241 tttctggctt ctgttccggc gaactggcct gcgtttgtgc agcggcaggc gttacgccct
    12301 catgcgcagc ttcattatct ttcaccaccg ggttatggat ggtgtgcgct tcatcgctcg
    12361 ccttttcagg atcgtttgca acgtaggaac gcttcatcga ctccgcggcc tggcgtagtt
    12421 catccatcga cgctttcagt tcgggcgtca ggttagtgag gctcgccttt tcaacctttt
    12481 tcagactgtc ctgaaactcc tggagtttta actcctgggt cagttcgttc tgcaccgttg
    12541 tcgccagtga acgcaacgcg cgaatccagc ccgctaccgt ttttaccgcc acaggcagtc
    12601 gttgcggccc cagaacgacg aggccgatga tgaacaccaa tagcagttcg ctaaaaccga
    12661 tatcaaacac ggattacacc tgctctttat cgtggcgctt cgcgtcttct gttttagcct
    12721 gttcctgatt cgtatccgcc tgcttatcgg cgatagtttt cgcagtaaaa tcagcatcct
    12781 gactggtttt atcctgcttt ggttcatcat cgctcattgc ttttttaaag cctttgatcg
    12841 acgcaccaag atcggaaccg atggagccga gctttttggt gccaaaaagc agtacaacga
    12901 tgacggcaat aatcaataac tgccaaatac tgataccacc catacatgtt cctctgtggt
    12961 agatgatgat taaacaaggc cgcattatac gttacacgac ccgccttgag cgatgaaaaa
    13021 atcagcgtgt tttgcgccaa ccgacaaacc aggcgatcag accacctgcc attaaccagc
    13081 cgggcatcag cccccattca ggtcggctga ccaacaagaa tgtgccactt aatactaacg
    13141 tagcgccaat tccgagaaaa taacgcgatt gtccctgacg tacatgattt gactgaagct
    13201 cgcgggcaat cttatcaaca ctgtgctgta aatacttgcc ctggcgcaaa ctgtcgtaaa
    13261 ccaattcagg cagttctggc attttttcga cccagaacgg cgctttttct ttaaatgctc
    13321 tcaccagggc aggaataccg acctgatctt taatccacga ctccaggaaa ggcttcgctg
    13381 ttttccataa atcgagctgc ggataaagct ggcgtcctac cccttcgaca tagagcaggg
    13441 ttttctggag taacaccagt tgcggctgca cttccatatt gaagcgacgc gccgtattaa
    13501 acagatttaa cagtacatgt ccaaacgaaa tttcggccag cggtttctca aagataggtt
    13561 cacagaccgt acgaatggca aattcgaact cttcaacgtt ggtatctggt ggcacccagc
    13621 cagaatcgac gtgtagctct gccactttgc gatagtcgcg attaaagaag gcgataaagt
    13681 tttctgccag atagcgttta tcttctttgt ttagcgagcc aacaatcccg caatcaatgc
    13741 cgatatattt cgggttttcc gggtgttcat agcttacgaa aatgttgcca gggtgcatat
    13801 cggcatggaa aaagctgtcg cgaaagacct gagtgaagaa cacctgcacg ccacgttccg
    13861 ccagcaattt catgttggtg ccatttttct ccagcgtcgc aacatcagac accggaatgc
    13921 cgtaaatgcg ctccatcacc atcatccctt cactacaata gtcagggtaa acttccggga
    13981 tgtagagcat cgggctgtct tcaaaatttc gccgaagctg aatggcgttg gcagattccc
    14041 gcagcaaatt cagttcatca atcaaggtct tttcgtactc gcgcaccact tcggttgggc
    14101 gcagacggcg accatccggc agcaaacgcg gcacccagcg agccagacgg tagataagtt
    14161 tcagatccgc tttaataacc ggcaaaatat ccgggcggat gactttaatc accacctctt
    14221 taccattcga tttcaatcgc gcggtatgaa cctgggcgat agaagcagaa gccagtggct
    14281 tgatttcaaa atcgtcaaac cacgcttcta ccggcaagcc gcccattgca gcttcaatct
    14341 gctgcttcgc cagcttgcca tcaaacggag caactttgtc ctgcaataac gccagctgat
    14401 cggcaatatg cggtggaaaa agatcgcggc gggttgataa catttgcccg aacttgatcc
    14461 aaaccggccc cagttcttgc agggccagtc gtagtcgctc acctaaaagt ttgtctttat
    14521 gccgatttgg catccagaat aatgagtatc gccatagccg tagcggcagg gtgatacgca
    14581 ttttggggat cagttcatca agtccgtagc ttaaaaaagt gcgaatgatg aaatataggc
    14641 gccgtacttc acctggcgtc atttagcctc cagtttttcc agccgtttgg tcagggcatc
    14701 aacagcacgc tcgacggcag ccgtctcttc cgcaaaccag gccacttcaa gcggaccggg
    14761 tgccatacgc cactcttcag taatggcttc cgccacataa cgttgctggc gcttaatgcc
    14821 gtgatgcagg aactttgcgc ctccgcgcat ggctttgctg attccttcag cggcgatatc
    14881 accggtataa ggggccagca gttccgcagg gtcgaactct gccagatctg ccagcgcaac
    14941 gaagttttgc accacctgaa tatcgccctg cacttccagc tcaccactgc gaatcagtgc
    15001 ggtaagctgc tggcgatcgc gaagtttcgg caacacactg gcgtaggcga taacggtgca
    15061 gtcagcatcg cctgcccatt cgcccagtac atcaacctgg cgttcgctga acaccagaat
    15121 caatgacgtc gaaaagcctt ttacctccac gcgcaatact ttacccagca gacgcgagcg
    15181 ggctgttttc agcgcgggtg agcgatacag gaaggtgttg agcagacttt caattcctgc
    15241 cgtcactaaa ggtttaaaag gcatttccgg tctcctgtca gaacttataa ccacgatgca
    15301 gcgccacaac ccctgccgtc agattgtagt agtcgacact ttcgaatccg gcatcctgca
    15361 tcatggcttt cagggtatcc tgatcgggat gcatacggat ggattctgcc agataacggt
    15421 agctgtcggc gtcgttcgcg accagtgagc caatacgcgg cagcacatgg aaggagtatg
    15481 catcataggc tttgctcagc ggctcgataa ttggcttcga gaactcaagc accagcaggc
    15541 ggccgccggg tttcagcacg cgatacattg aacgcagtgc tttatctttg tcggtgacgt
    15601 tacgcagacc aaacgaaatg gtgatgcaat caaaggtgtt atccgggaac ggcagcgcct
    15661 cagcgttcgc ctgaacatac tcaacgttgc caatcacacc gatattacgc agcttctcgc
    15721 gtcccatttt gagcatggat tcattgatat cagcaaggac cactttgcca gtttctccga
    15781 ccaggcggga gaatttcgct gtcaggtcgc cggtgccacc agccagatcc agcacggtct
    15841 gcccacggcg tacgccgctg caatcaatcg tgaatcgctt ccacaaacga tgaataccaa
    15901 atgacatcaa atcattcatg acatcgtatt ttgatgccac ggaatggaaa acgtgggcga
    15961 ccatatccgc tttttgttcc ttcgcgacgg tctgaaaacc aaagtgcgtc gtttcttgtg
    16021 acttatccac catctcaatg cctgctcatc aaaaaattgt tccagaagtg taacagattg
    16081 ggcgtcgatg ccctagattt ctacccggct taactacccc taatgggcta gcgcgactgc
    16141 tgattatatt catcatcgcg ttgataagct tcatcattcg gctgctccgg aaccgaccga
    16201 agtcgatact cttcattctg gctcaccgcc tgttcagcca aatccggatt aatctcgcgt
    16261 ttaatttcta ctcctaaacc gcgaaacgct tctgcctgcg ccagcacatt tccgcgacct
    16321 gaagagagtt ttttcattgc ctggcggtag ttatcctgcg ctttgtcgag actttgacca
    16381 atcgcggaca tgtcatcgat gaacaaacgc atcttgtcgt acagcttgct ggcacgatcg
    16441 gcgatttgct gggcgttgcg gctttgatgc tcataacgcc acaggttggc aatagtacgc
    16501 aacgccacca gcagcgtagt cgggctaacc agcatgatat tgtttttcaa cgcttcggtg
    16561 atcagctccg gctggcggtc aagcgccagt aaaaaagcgg gttcaacggg aataaacatc
    16621 agcacgtaat ccagagttcg cagccccggc agctgttgat aatctttgcg tcccagcaaa
    16681 cggatatggt tacgcaccga cgcgatatgt tcctgtagcg cgctttcgcg ggtgtagtcg
    16741 tcttcggcgt taaaatagcg ttcataggcg accagcgtca ttttggcgtc gatcaccaca
    16801 tcttttccct gcggcagccg cacgatgaca tccggctgca tccgcgagcg ggcgtcattt
    16861 tcgatgctga cctgggtttc atattcatac cctccacgca gaccggaagc ctccagcacc
    16921 cgcgtcaata ctacctcgcc ccagttgccc tgggttttat tgtcgccttt cagcgcgcgc
    16981 gtcaggttga tcgcttcctg ggccatttgc gcgttgagtt gctggagatt gcgaatttcg
    17041 tgggtcaggg tatggcgttc ttgtgcttct ttaccgaagc tgtcctgaac ctgacggcgg
    17101 aaaccgtcca gttgttcacg tagcggcgac aacaggctgt tcagactctg acggttttgc
    17161 tcatcaaccc ggcgattgct gtgctcaaaa atacggttgg cgaggttttc aaactgctca
    17221 ctgaggcgct gctcgctgtt aatcatctgg cgaattttat cgtcagcatg ttgctgtgcg
    17281 gcttccatcc gcgtggttac ttcacgcaga tcggcctcca gagaggtgtt aatactttgc
    17341 aggctgcgca cttcgttatt gagtaactcg cactctgcac gccagtgctc gctttgggta
    17401 atttgttgtt ttgccgcgct taactccgcg accatctctt cacgttcagc taattgctcg
    17461 gctttttgct gcgcatgttg ataactggca aacagccagc caattgccac acccaccaac
    17521 gcaataactg cgtaaaccat gattgagaaa tccacaacgc ctcctgacgc aacaacttac
    17581 ccgcacaaaa tagaattgtg ctgtacaaac gtccagttgg aaaggcattt cctgcgaggc
    17641 gctacgcaaa aatacaaaag gccgaacgcg tcggccttca gacaggagaa gagaattaca
    17701 gcagacgacg cgccgcttcc accacgattt tcaccgcttg gctttcagtt tgtttcatcg
    17761 tctcagcatt cgggatctct tgctgggtgc ggttaacgat aacacccgct accataccgg
    17821 cacgcaggcc ctgacttgca cacatggtca gcagggttgc agattccatt tcatagttca
    17881 ttatgcccat cgcctgccac tcttccatag aacctttaaa gtgacgaact acgcgaccag
    17941 agtaagtatc gtaacgttcc tgacctgggt agaaggtatc agaagaagct gtcacgccaa
    18001 cgtgagttgt cgcgccaatg gatttcgcag cttcaaccag cgcagtcgta cattcgaaat
    18061 cagcgacagc cgggaattcc agcggtgcga agtgcaggct cgcgccatcc agacggacag
    18121 acgccgtggt aaccaggaca tcacccacat taatatgcgg ctgaatagcg cccgttgtac
    18181 cgatacgcag gaaggtgcga atgcccagct gtgccagctc ttcaacagca atagaggtag
    18241 acgggccgcc gataccggta gagcagacga taacaggttt accatccagc tctgcacgcc
    18301 aggtagtgaa ttcgcggtga gatgccagct taaccggctt atccatcagc gcggcgatct
    18361 tttccacacg atccgggtcg ccagggacga tggcaagcgt agccccttgt aaatcgtttt
    18421 tagtgaggcc gagatgaaaa acatcagact tggacatata caactcctct gtgaatcggt
    18481 ttagtcagaa gaagcaaaaa gacactttac cgaagggttt aacatttttt cgtgatactc
    18541 atcaccatga cgcaaatgcg ttgcataaat ttacccacca aaagtgattc aaatcacata
    18601 aatcactctt cattctgcag gactgcataa aagatgagcc gattcaggca atgtgatttg
    18661 ttgcatgctg actatagtta acattgcgca acatttttat aagggtacgg agaataccat
    18721 ggcaacaaca caacaatctg gatttgcacc tgctgcatcg cctctcgctt cgaccatcgt
    18781 tcagaccctg gacgacgcga ttgtggcggg cttcacctct atcccttcac aaggggataa
    18841 catgcctgct taccatgcca gaccaaagca aagcgatggc ccactgccag tggtcattgt
    18901 agtgcaggaa atttttggcg tgcatgaaca tatccgcgac atttgtcgcc gtctggcgct
    18961 ggaggggtat ctggctatcg cacctgaact ttacttccgc gaaggcgatc cgaatgattt
    19021 tgccgatatc cccacgttgc ttagcggtct ggtagcaaaa gtgcctgact cgcaggtgct
    19081 ggccgatctc gatcatgccg ccagttgggc ttcccgcaac ggcggcgatg ttcatcgttt
    19141 aatgatcacc ggattctgct ggggtggacg tatcacctag ctgtatgccg cgcataatcc
    19201 acagctaaaa gctgcagtgg cgtggtacgg caaactgacg ggcgacaagt cgctgaattc
    19261 accgaaacaa cctgttgata tcgcaaccga tcttaacgcg ccgattctcg gcttatatgg
    19321 cggtcaggat aacagcattc cgcaggagag cgttgaaacc atgcgccagg cgctgcgggc
    19381 tgctaatgcg aaagcagaga ttatcgtgta cccggatgcc gggcatgcat tcaacgctga
    19441 ttatcgcccg agctatcatg ccgaatctgc gaaagatggc tggcagcgta tgctggaatg
    19501 gtttaagcag tatggagaga agcggagtta gtttcctctt agtctatatt tgagatggtg
    19561 agtaaacgag tgcgttttgc tgccatctct gtcccccaaa aaataatcag tatgaattga
    19621 ttggagatta cgactccgct atttgacctt aagaaagtcg gcaagtcata gtcataaatg
    19681 agtctcttca atggaagaca ccttagagat ttaccatcaa ctcccattca caaacaaaca
    19741 ttaattatta tcaacacatt cacattaatt ataaatcatc tcagaccttc ctgataaaaa
    19801 gatattaaag tgaaacattt tgtttttcac attttagttc atcactttta ggcgtaaata
    19861 aaggtaaaaa aaacaacaat actatttctc tttcacaaaa gaattaacaa aaggtttttc
    19921 aaggatgagt gatattattt acctgaccat aaccggagaa caacaaggat gtatttcctc
    19981 tcgttgtgga acgagcgcat caataggtaa tcgctggcag attggccatg atgatgaaat
    20041 cttcgcgttc tcgctctcta atagtattac gaatacaggc aaagggtcat aattgcatgg
    20101 ccttagtttc tgtaaattga ttgacaaaag ctccccgtta cttattaacg ctattaacaa
    20161 taacgagcag ttgttcatgg agtttgactt ctatcggatc aataggtttg gtcgatggga
    20221 gaaatattac aacattcaac tccgcggcgc gctcttatca gcaatcaatc acctgtttac
    20281 tgagaacaat ctggatacag aagccatcac tgtcagttat taatatatac tctccaggca
    20341 tctgattgct aatactgaat ttagttattg ggcatttccc gataactaca accgtctgtt
    20401 tataccacgg ccaaaaacta aagctggtaa tggtttcaaa acacttaata gtaaagcggt
    20461 tggcagactg cttgctgctg tcggcatata caatgggaat atcgaaggat tcagggaaac
    20521 agcagacaag ttggggggag atgcacctgc tggttatgat caatttatgg ataacaaagg
    20581 gttactcatc gctggtgctt caatagttgc tggagcagtt ctggggagaa tgagactccc
    20641 tgatttggaa tcacttgagc atttcggagc cagaggagca gtatcaggaa gaccatttaa
    20701 tcccgagctt gccggtggtc caatagaaaa cctgacgaca gatggcgtaa caatcaacag
    20761 ggaaggtatc gctattgttg aaaaacatat agccagattt ggtcatgatc ctgtaaatga
    20821 agtgatgatc aatagactga aagacattga gaaaggaaaa atccccccag aacaagtaga
    20881 tcttaacttc tatactcatg agtgtcggga atatcagcgt tactgtaatc ttggttggga
    20941 aacaggacaa cctgatggtg acgcaggcta tgcactctgg aatcatactg gatttgcccc
    21001 tatatttcca gacatctgtt atcacttaac ccattacaag cccgctgcc
//
LOCUS       DAKISA010000078        21005 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.078, whole genome shotgun sequence.
ACCESSION   DAKISA010000078 DAKISA010000000
VERSION     DAKISA010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21005
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.078"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..1173
                     /gene="yicI"
                     /locus_tag="OVW45_002905"
     CDS             <1..1173
                     /gene="yicI"
                     /locus_tag="OVW45_002905"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCV5527304.1"
                     /translation="GLAIYDFTNPDACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQ
                     WFDGSDPQKMHNHYAYIYNELVWNVLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCY
                     ANYESMAESLRGGLSIGLSGFGFWSHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGS
                     KSYRVPWAYDDESCDVVRFFTQLKCRMMPYLYREAARAKARGTPMMRAMMMEFPDDPA
                     CDYLDRQYMLGDNVMVAPVFTEAGEVQFYLPEGRWTHLWHNNELDGSRWHKQQHGFLS
                     LPVYVRDNTLLALGNNDQRPDYAWHEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKA
                     TRTGNTITVTGAGEARNWTLCLRNIVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(1225..2934)
                     /locus_tag="OVW45_002906"
     CDS             complement(1225..2934)
                     /locus_tag="OVW45_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCV5527305.1"
                     /translation="MKFIGKLLLYILIALLVAIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMVHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSAE
                     LVQ"
     gene            complement(3055..4446)
                     /gene="xanP"
                     /locus_tag="OVW45_002907"
     CDS             complement(3055..4446)
                     /gene="xanP"
                     /locus_tag="OVW45_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCV5527306.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            4726..5931
                     /gene="gltS"
                     /locus_tag="OVW45_002908"
     CDS             4726..5931
                     /gene="gltS"
                     /locus_tag="OVW45_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCV5527307.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(6100..8181)
                     /gene="recG"
                     /locus_tag="OVW45_002909"
     CDS             complement(6100..8181)
                     /gene="recG"
                     /locus_tag="OVW45_002909"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCV5527308.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(8187..8876)
                     /gene="trmH"
                     /locus_tag="OVW45_002910"
     CDS             complement(8187..8876)
                     /gene="trmH"
                     /locus_tag="OVW45_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCV5527309.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(8883..10991)
                     /gene="spoT"
                     /locus_tag="OVW45_002911"
     CDS             complement(8883..10991)
                     /gene="spoT"
                     /locus_tag="OVW45_002911"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCV5527310.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(11010..11285)
                     /gene="rpoZ"
                     /locus_tag="OVW45_002912"
     CDS             complement(11010..11285)
                     /gene="rpoZ"
                     /locus_tag="OVW45_002912"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCV5527311.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(11340..11963)
                     /gene="gmk"
                     /locus_tag="OVW45_002913"
     CDS             complement(11340..11963)
                     /gene="gmk"
                     /locus_tag="OVW45_002913"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCV5527312.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            12221..13903
                     /gene="ligB"
                     /locus_tag="OVW45_002914"
     CDS             12221..13903
                     /gene="ligB"
                     /locus_tag="OVW45_002914"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCV5527313.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDSVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQRLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(13900..14517)
                     /locus_tag="OVW45_002915"
     CDS             complement(13900..14517)
                     /locus_tag="OVW45_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCV5527314.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIAIATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(14809..15633)
                     /gene="dinD"
                     /locus_tag="OVW45_002916"
     CDS             complement(14809..15633)
                     /gene="dinD"
                     /locus_tag="OVW45_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCV5527315.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSINQLENSVKITEKK"
     gene            complement(15855..16718)
                     /locus_tag="OVW45_002917"
     CDS             complement(15855..16718)
                     /locus_tag="OVW45_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCV5527316.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            16845..17561
                     /gene="rph"
                     /locus_tag="OVW45_002918"
     CDS             16845..17561
                     /gene="rph"
                     /locus_tag="OVW45_002918"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCV5527317.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKSQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            17626..18267
                     /gene="pyrE"
                     /locus_tag="OVW45_002919"
     CDS             17626..18267
                     /gene="pyrE"
                     /locus_tag="OVW45_002919"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCV5527318.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KNHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RSEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(18304..18900)
                     /gene="slmA"
                     /locus_tag="OVW45_002920"
     CDS             complement(18304..18900)
                     /gene="slmA"
                     /locus_tag="OVW45_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCV5527319.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(19007..19465)
                     /gene="dut"
                     /locus_tag="OVW45_002921"
     CDS             complement(19007..19465)
                     /gene="dut"
                     /locus_tag="OVW45_002921"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCV5527320.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(19443..20663)
                     /gene="coaBC"
                     /locus_tag="OVW45_002922"
     CDS             complement(19443..20663)
                     /gene="coaBC"
                     /locus_tag="OVW45_002922"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCV5527321.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEVAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            20835..>21005
                     /locus_tag="OVW45_002923"
     CDS             20835..>21005
                     /locus_tag="OVW45_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5527322.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYG"
BASE COUNT         4818 a         5387 c         5718 g         5082 t
ORIGIN      
        1 ggtctggcga tttatgactt taccaatccg gatgcctgca aatggtacgc cgacaaactg
       61 aaaggtctgg tcgcgatggg cgttgattgc tttaagaccg actttggcga acgtatccca
      121 actgatgttc agtggtttga cggttccgat ccgcagaaaa tgcataacca ttatgcgtac
      181 atctacaacg aactggtgtg gaacgtgctc aaggacaccg ttggtgagga agaagctgtc
      241 ttgtttgccc gctcggcctc cgtcggtgcg cagaaattcc cggtacactg gggtggcgat
      301 tgttacgcta actacgaatc aatggcggaa agcctgcgcg gtggtttgtc tattggcctt
      361 tcaggttttg gcttctggag ccacgatatc ggcggctttg aaaataccgc gccggcacac
      421 gtttacaaac gctggtgcgc gtttggtttg ctctccagcc atagtcgttt acatggcagc
      481 aaatcttatc gtgtgccgtg ggcctacgat gatgagtcct gtgatgtggt gcgcttcttc
      541 acgcaactga aatgccgcat gatgccgtat ctgtatcgtg aagctgcgcg tgcgaaagcg
      601 cggggtacgc cgatgatgcg ggccatgatg atggagttcc cggacgatcc ggcttgtgat
      661 taccttgacc gtcaatacat gctaggcgac aacgtgatgg ttgctccggt gttcactgag
      721 gcgggcgagg tgcagttcta cttgccggaa ggtcgctgga cacacctgtg gcacaacaat
      781 gagctcgacg gtagtcgctg gcataaacag cagcacggct tcctgagtct gcccgtttat
      841 gtgcgtgata acaccctatt ggcgctgggc aacaacgatc aacgtcccga ttacgcgtgg
      901 cacgaaggca cggcattcca tctctttaat ctgcaagacg ggcatgaagc tgtatgtgaa
      961 gtgcccgctg ctgacggttc ggtgatcttt actctaaaag caacacgtac tggtaacacg
     1021 attactgtga ctggtgcggg cgaggcgagg aactggacac tgtgcttgcg caatattgtg
     1081 aaagtaaatg gtctgcaagg cggttcgcag gctgaaagtg agcaggggct ggtggttacg
     1141 cctcaaggga atgcgctgac aattacgttg taatctattg aagaatgccg ggtacgtctg
     1201 agtcgtaccc ggcgagattg atgtttactg caccagctct gccgatgtaa cttcaccgct
     1261 ggaaacgacg cccgcattca tggtttgagt cacttgctgc tttgcggcgt tcacggcatg
     1321 caattgcccg ctaaccgtag gtttcaacgg gacattggcc tgaatatcgc cactggcggt
     1381 aagctgaata ttgccgtcgc cagtcaacgg taattcaggc catccccact gttgcaaaat
     1441 attcaccggc acaccgcgtc cattcaggct gatatgtgtc tgacgttgtg gcgtttgtga
     1501 aacactggcg gtggcttcca gaatgccttt ttcagtaaat gcactcagtt cgctgatatt
     1561 caccgtgctg ctgttggcgg ttagcgccag cgacggacga cggacatcaa cacgattaaa
     1621 cgtcgcggcg gcggcattca gattcgccga gccactccag acgccccatt tatgatcggt
     1681 aaccagcgtc aggttggcac cgtaaccatc gagcgcggtg agctgccacg ggaagtcagg
     1741 gtcgatatca atgatcagat tgcggctggc gctaaatctc ttcagttgca ggctgtttaa
     1801 ccaaccgggt gtcgtttcca tccacaactg ttgccagttt ttcggcaagg tatattccag
     1861 tccggcaatt gccgcatcat caaggatcaa cgttttcccg tcacgcagcc agttccctga
     1921 cgttctgacc ataccccctt cccagcggct ggtgaactgg cgcagcgcta cgccctgcgg
     1981 agaaaattcc gcgtttataa tcgggtcaaa taaatgcagc gaaccataaa tgaactcgct
     2041 agcgttcatc gacagtttgc cttcttgtgt ctgccagtca tctttactga aggtcatgtt
     2101 gcgcaagctg agatcgaggt cggtcaccgc ccagtccgga ccttgcaaac gagcatcgat
     2161 cacttccagg cgaccaatct gcaacgacgg gacagagcgt aatggcgcaa agaagtcggt
     2221 tagcgatttt tcgctttgta gacggatatc cgccatgcgc aggttttcca cttgccagct
     2281 gccgtcggcg ttacgctgcg cgtttccggt taatgtcccg cgcgcgatgt cggcacccag
     2341 gttagtcagc gtaacgcgat cgttatcaat actgccttca atcagtacat tggtggcagg
     2401 aacatcattg agcgaaagcg atccggcact aaactgaatc tgcgccttcg tacccagcac
     2461 tttaccggct tctggtgacc acggaaccac gccgccattt acccgctgcg cgctcagttt
     2521 ccattcgcta ttcgggctat taaacgccat atcacgcagt tgcagacgat cggctttgaa
     2581 cggtagcggc gcggtctggt cggtgagatt cagcgtcccg ttttccagca ggatggtatc
     2641 gacatggcgt ggttcggtca gttgccgact acttagcgca atgtcgacac ttttggccac
     2701 cagggtcgcg ggctggccat cacgaccaaa cgtgacattc tccagcacga tatgagatgg
     2761 cgcggaaaaa cggtgaacca tcgccccgaa ggccagatga tagtcgctat tctcggaaac
     2821 ccatgcgctg atatgttctg ctccccagcg ggtttgcaga agaaaataga ggccagcgat
     2881 cgccactaac agagcgatga gaatgtagag aagcagcttc ccaataaatt tcatggtctt
     2941 ccatcccgtg aaatgcacat aagggagtta tgcacgattt acgcgcaatc ctcaaggcgg
     3001 gaatggttaa agaggtgaga ctgcggcggt aattatctta ccgccgccag tgaattactg
     3061 tttttctggt gggaaaatca gattcagcac gatggcagta ataccgcccg cggcgatccc
     3121 ggaggagagc aggtttttca gccattcagg ggcaaactgc aaaatcagcg gctgctgaga
     3181 cacgcccaga ccaaccgcca gcgacagcgc gataatcaga atcgcccgac ggttcagcgg
     3241 ctcacgagaa acgatacgca caccggaggc ggcgatggtg ccaaacatta caagcgttgc
     3301 gccgcccaga accggttctg gaatgtgttg tacaaaaccg ctcactgccg ggaacagacc
     3361 cagcacgatc aacatcagcg cgacgacaaa accgacatag cggctggcaa caccggtcaa
     3421 ctggatcact ccgttgtttt gcccgaagca ggagttcggg aaggtgttaa acaccgccga
     3481 aacaaacgag ttcaggccgt ttgccagcac gccgcctttc aggcgtttca tgtacagcgg
     3541 accggacact ggctgttcgg aaacgtcaga ggtcgccgtg atatcgccaa tggtttccag
     3601 cgaagtgatc ataaagacca gcatcagcgg cagcagcaga ctccattcaa tgccaagacc
     3661 gtaatagagc ggcgttggca ccataatcag ttcttgcgtc atcggttcgt tgctttctgg
     3721 caacatgccc ataaaccacg ccagcgcata tccggccgcc atcgcaatta ccagtgaggc
     3781 cacgcgtaag taagggttac gttgacggtt aagcaggata attaaggcta aaaccacgcc
     3841 tgccagcagc agatttttcg gtgcgccgaa ggtgttatcg ctcatggctg cgtaaccgcc
     3901 gccaatggag gtcagcccca cctgaattag cgacaggccg ataatcatca ccacaacgcc
     3961 agaaaccagc ggcgtaataa tgcggcgcgc cagatgcaga acgcgggaga tcaccatctc
     4021 ggtgcaactt gccagcatca acgtgccgaa caaagccgcc atcatggtag gaacatcagc
     4081 accaccggtt ttcagcgcgg taccgcccat aatcagcggg gcaacaaagt tgaagctggt
     4141 gccctgaata gacaacagcc cggagccaac cggaccccag gccttaattt gaataatcga
     4201 tgccacaccg gaggcaaaca gcgacatact aataatgtgt tgcgtgtctt gtgccggtaa
     4261 acccagcgcc tggcagatta atagcgctgg cgtgatcacc gcaacgaaca tcgccagcag
     4321 atgctgacag gcggcaaaca gggtttgagg aagcggcgga cgatcttcaa gacggtaaat
     4381 cagttcgctg ttttgagtct gcgcaaccgg ttgcgcattt tctgactcga gggtggaaac
     4441 agacatcgaa aacaatcccg tggtagcaaa gcgggcattt tagctgaccg aaagagaaaa
     4501 gcaaacgttt gccacatcct catctaaata tttaaatgag ataaatgcac ttttttatat
     4561 aacttttggt atttttctgc ttaaaatcca tgccatttgt tcattaacag ctgagaattt
     4621 gacccaggaa agcataactc gatacccgtc atagttcatg aagttgccac tgcgcgatga
     4681 agtatgacga gtatgaaaga gtgatgcgga tacaaaggag taactatgtt tcatctcgat
     4741 actttagcaa cgcttgttgc cgcaacgctg acgttgctgc tcgggcgtaa gttggtccat
     4801 tccgtctcct ttttgaagaa atacaccata ccggaacctg ttgcgggtgg tttgttggtg
     4861 gcgctggcgc tactagtact gaaaaaaagc atgggctggg aagtcaactt tgatatgtcc
     4921 ctgcgcgatc cgttaatgct ggctttcttc gccaccattg gcctgaacgc caacattgcc
     4981 agtttgcgtg ccggtgggcg tgtggttggc atcttcttga ttgtggttgt tggtctgttg
     5041 gtgatgcaaa atgccattgg cattggtatg gctagcctgt tagggcttga tccgctgatg
     5101 gggctgttgg ccggttctat tacgctttcc ggcggtcacg gtacgggcgc tgcgtggagt
     5161 aaattgttca ttgaacgtta tggcttcacc aatgcgacgg aagtggcgat ggcctgtgca
     5221 acgttcggtc tggtgctggg cggcttgatt ggcggtccgg tggcgcgcta tctggtgaaa
     5281 cactccacca cgccgaacgg tattccggat gaccaggaag tcccgacggc gtttgaaaag
     5341 ccggatgtgg gacgcatgat cacctcgttg gtgctgattg aaactatcgc gctgattgct
     5401 atctgcctga cggtggggaa aattgttgcg caacttttgg ctggcactgc ttttgaactg
     5461 ccgaccttcg tctgtgtact gtttgttggc gtgattctga gcaacggtct gtcaataatg
     5521 ggcttttacc gcgtctttga gcgtgcggta tccgtgctgg gtaacgtaag cttgtcgttg
     5581 ttcctggcga tggcgttgat ggggctgaaa ctgtgggagc tggcttcgct ggcgctgccg
     5641 atgctggcga ttctggtggt acagaccatc ttcatggcgt tgtatgccat cttcgttacc
     5701 tggcgcatga tgggcaaaaa ctacgatgcg gcagtgctgg ctgcaggtca ctgtggtttt
     5761 ggcctcggtg caacgccaac ggcaatcgcc aacatgcagg cgattactga acgctttggc
     5821 ccgtcgcaca tggcgttttt ggtggtgccg atggtcggtg cgttctttat cgatatcgtc
     5881 aatgcgctgg taattaagtt gtatttgatg ttgccgattt ttgccggtta accgatgaag
     5941 cggcggtaga agtgccgccg caacaaagac aaatgcctga tacgcttcgc ttatcaagcc
     6001 tgcgtattga ttcataattt attgaatttg tagggtgatg aatcgcatcc ggcaggaagg
     6061 tagggtaacc tgaaatggcg gtcttctcac tgccgccttt tacgcattcg agtaacgttc
     6121 cgtctccggc atccagcgtt ctatcagggc ttttgcctgt tgtgggtaac gttcgtgaat
     6181 atggcgtgcc aggcgctgaa cttccgggat catcgcctga tcgcgcagta aatccgccac
     6241 tttaaattca gcattacccg tctgacgcgt gcctaacaat tcgccagggc cgcgaatctc
     6301 cagatctttt tgcgcaatca caaaaccgtc gttactgtcg cgcagcactt gcaggcgaat
     6361 ttgcgccgtt ttagaaagcg gcgttttgta gagtagcacg cagtgagaag ccaccgcgcc
     6421 acgacctacg cgcccgcgca gctggtgtaa ctgcgccaga cccagacgct cagggttttc
     6481 gataatcatc aggctggcgt taggcacatc aacaccgact tcaataacgg tagtggcaac
     6541 cagcagatgt agctcacctt gtttaaacga cgccatcacc gcctgtttct cggctggttt
     6601 catccgcccg tgtaccaggc caacattcaa ttctggtagc gccagtttca actcttccca
     6661 ggtagcttcc gccgcctgcg cttccagtaa ctctgactct tcaatcaacg tacaaaccca
     6721 gtacgcctga cgaccttcag tcatgcaggc gtggcgcacg cggtcaatga tgtcggtacg
     6781 gcgagtatca ggaatagcga ccgtagtcac tggcgtgcgg cctggcggca gttcatctat
     6841 caccgaggta tcgagatcgg catacgccgt cattgccagc gtgcggggga tcggcgtggc
     6901 ggtcatgatc aactgatgcg gatggaagcc ttgctgctgg cctttctccc ataacgccag
     6961 acgctgatgc acgccaaagc gatgttgttc gtcgataatc accagcgcca gaccgttaaa
     7021 ctgcacctgt tcctggaaga tggcgtgcgt accaacaatc atctgcacct gaccgctggc
     7081 gatggcttcc tgctgagaca gtcgcgcttt gcctttctgc ttaccggcga gccagcccac
     7141 ttcgataccg agcggttcaa accagttgcg gaagttattg gcgtgctgtt cggcgagtaa
     7201 ttcggttggt gccatcaatg ccacctgttt gccgtgggca atcgcacgca gcgcggcgag
     7261 ggcggcgacc agcgttttac cggaacctac atcgccctgt accaggcgca tcatcggcac
     7321 atccagcgcc atatcgcgct cgatctccgc cactacgcgt gcctgtgcgc ccgttggctt
     7381 gaacggtaag gctgcgagga gtttattttt cagcgcatca ttggcgctca gcggctgggc
     7441 atgaaaacgc tgtgctccgg cacgtaaggc taacatgctg aggttgtgcg ccagcagttc
     7501 ttccagaatc agacgacgtt gcgccggatg ctgcccggtt tccagatcgc taagctgtag
     7561 cgtcggtggc gggcggtgca aagtgcgcaa cgcttccggt agcgtcatca ttccttgtga
     7621 cagttccggc ggcaggagtt cttcaatggc gcaggtgtcg agcagatcca gcgcctggtc
     7681 ggttaattta cgcagcgtgg cctgctttac gccttccgtt gttggataaa ccggcgtgag
     7741 cgtttcctgt aattctggcg tgctgagatc gccctgcacg cggtattccg ggtggatcat
     7801 ctccgcacca tatttaccgc gctttgcttc gccataagcc agtacacggc ggcccgtcgc
     7861 caggctattt ttcattgccg cgctgaaatt gaaaaagcgc atggtgagga tgccggaacc
     7921 gtcgctgatc tggcaggtca tcatccgccg accgccgaag gagatattgc agttcagcac
     7981 ttcgccttcc accgtggcat aaacgcccgg cagtagttct ccgatggggt agagatgggt
     8041 gcgatcttcg tagcgcaggg gaaggtgtaa gagtagatcc tgcacggtat gcaggttgat
     8101 tttcgccagt ttgttactaa gtgctgcgcc aacgcccgtt agggaactga gtgggacagc
     8161 atctaacagg cgacctttca tggcacttac cctgcagcct gcatagtagc ccaccagtcg
     8221 gcatcagctt cgatctcgcc ttgctgattg acgtggggat aaggcaggcc tttgcgtttt
     8281 gcgactttcg ccagcaccgg atagccgcct tcaaacaaca ggcgttgttg ctctgcttcc
     8341 ggcaacatgc tgttttcacg caggtacatg cctgcatttt gccgctgacg ctgggcttcg
     8401 taaagaatga gggctgaggc aacggaaaca ttcagcgact gcaccatgcc gatcatcgga
     8461 atgatgatgt cctgatccgc cagggccaat gcttcctgcg tgatgcccgt tttctcctgt
     8521 cccatcaaaa tgcaggtcgg gcgagtgtaa tcaatttcgc ggaaatcgac agcgttatca
     8581 gaaagatggg ttgccagaat ctgcatgccc tggcctttca gatgagcgac ggcatcgcca
     8641 atggtgcggt gtgttttcac ctgtacccag ctgttactac ccgccgctgc cgaagccatg
     8701 gtgcgcatgc ggctaccagg ccagacggcg tgaacttcat gtacgccaac ggcatctgcg
     8761 gtacgaataa tcgcagaaac gttatgaggt ttgtggacct gctccatgca gacggtcaga
     8821 tcaggctgcc gcctggcgag catttcgcag atgcgtgcat aacgtgttgg gttcataaaa
     8881 cattaatttc ggtttcgggt gactttaatc acgtctggca tcacgcggat tttgcgcatg
     8941 atattcgcca gatgcacacg gtcacgggcg gtcagacgaa taaaggcgct gtagacgcga
     9001 ccatctttct cttccgtatt caaactttga atattcgaag tcgtggtgtt aattgccgcc
     9061 gtcaggtttg ccagcgcgcc ctgatgattg aacatctcca ccttgatttc ggtgatgaac
     9121 tcctgcgccg tctctttatc ccattccaca gccataaact tctctggctc tttctggtag
     9181 ccacggatat tacggcagga ttcatggtgg atcaccagac ctttaccggg gctgacgtgg
     9241 gcgataatcg ggtcgccagg aatagggcgg cagcatttcg caaaggtgat cagcacgcca
     9301 tcggcacctt taatgggcag atgtccgtgg ctttgggttg ccggtggaat ggaggcgtcc
     9361 ccatgttgca gatttttcgc gaccaccacg ctcattgcgt taccaagtcc gatttctgcc
     9421 agcagatcgt caagcgttgc cagcttcatg cgatccagct cgcgctgaat attttcctgc
     9481 gggatttcat tcagcttacg gctaccaccc aaagcatggt tgagcagacg acggcccagg
     9541 cttacagaat catcacgctt gaggtttttc agcaactgac gaattttggc gcgcgctttt
     9601 gagctaacga caaagttcag ccacgcggca ttcgggcgag cgcccggtgc ggtaatgatt
     9661 tcaacggttt gaccgctggt aagcggctgc gacagcgggt aaggctggcg gtcaacgcgt
     9721 gcgcccacgc aggcatgacc gatatcggta tgcactgcat aagcgaagtc gacgggcgtt
     9781 gcaccggcag gcagctcgac aattcgccct tccggtgtga aaacgtaaat ctcatccggg
     9841 aagagatcgg atttaacgct ctcgataaat tcaaacgaac taccggcgct ctgttgcagc
     9901 tccagcaggc tttgcatcca gcgctgggcg cggatttgcg cggtggtgct ggtttcgccg
     9961 tgctctttat aagcccagtg cgcggcaaca cccatctccg ccatctgatc catatcttcg
    10021 gtacggatct ggacctcaac cggcacaccg tgcgggccga tcatcgaggt gtgcaaagac
    10081 tgatagccgt tcgcttttgg aatggcgatg tagtctttca cgcgacccgg acgcggcttg
    10141 tacaggctat gcatttgccc gagcacgcga taacaggtgt cggaatcatt gacgatcacg
    10201 cggaaagcgt agatgtccat gattgagtga aaacgctgct ctttgagcac cattttgcag
    10261 taaatcgaat aaagatgctt ttcgcgacca ctgacgcggc acggtattcc cgcttcctgc
    10321 aaacgccctt cgatttcaga aagaatcttc tggatcatct ctttacggtt gccgcgcgcg
    10381 gctttcacca cttctttgat tacgcgataa cggttgggat acagcgcctc aaaacccagc
    10441 tcttcgagtt cggttttaat gtggtggata cctaaacggt gcgccagcgg gctgtaaatt
    10501 tcgagagttt cacgggcgat gcggcgacgt ttgtccgggc gaagtgagcc cagcgtgcgc
    10561 atgttgtggg tacggtcggc aagtttgatg aggatgacgc ggatatcctg caccatcgcc
    10621 ataatcatct tgcgaaagtt ttcggcctgc gcctctttct tatcgcggaa cttgagttta
    10681 tcaagtttcg ataccccctc taccagctcg gcgacgcttt taccaaaaag ctgttccata
    10741 tcctggtagg tggcgggtgt atcttcaatc acgtcatgca gcagcgccgc catcagcgtt
    10801 tcatagtcga gtttcatctc ggccagaatg caggcaaccg ctaccgggtg cgtgatgtag
    10861 ggttcaccgc ttgaacgtgt ttgcccctcg tgagcatcac gtgcaacgag atacgcctgc
    10921 cgcagacgct tgatttggtc ttccggcagg taggtttgaa tcagttgatt caggctttca
    10981 aacagataca agggcgaccc gctttgtgat taacgacgac cttcagcaat agcggtaacg
    11041 gcttgtaatt cagcggcttc ctgctcttgc tgttcctggc gttcgcgaac gtcgaggatc
    11101 tggttgttga tcagaccttc ttcgatttcg cgcagcgcga ttacagtggt tttatcgttt
    11161 tcttccggta ccagcggatc ctttccgcct acctgcatct gacgagcgcg acgcgcggcg
    11221 accagtacca ggtcaaaacg gttaccaatt ttctctacag cgtcctgaac agttacgcgt
    11281 gccatactta aaaagctcca caggtgaaga aatgactggg catgatactg aaatcaggtt
    11341 cagtctgcca acaatttgct gattaaagcg tcatgacgct gcttttggcg gctcatgcgc
    11401 agacgttcgg cgcgaataat ggtcttcaaa tcggtcaacg cggtatcgaa gtcatcattc
    11461 acaatcagat aatcatattc ggcgtaatgg ctcatttctg caacagcttg cgccatacgc
    11521 tttgcaatga cctcttcgct gtcctgaccg cgaccgcgta gacggcggtc cagttcaatt
    11581 ttggacggcg gtaaaataaa gatactccgc gcgtgcggca tcttctggcg aatttgctgc
    11641 gcgccctgcc agtcgatatc gagaaaaaca tcgacaccgg tcgccagtac ttgctcaatg
    11701 gcctcacgcg aagtgccata gtaattacca aaaacttctg cgtgttcgag gaacgcatct
    11761 ctgctaatca tttctttaaa ttcatcatga ttaacaaaga aataatgttc accgtggact
    11821 tcaccaggac gcggttggcg tgtggtgtgt gaaacagaaa cctgggtgtc atacaacggt
    11881 tgggttttta ataaagcctg aatcaggctg gatttacccg cgccactggg ggcagaaaca
    11941 atataaagcg tgccttgagc catgagtatc tttcgtatgt gattagcgaa ataaagccta
    12001 catacgagct tattatacac ggcgcggcca tgtgacgtag cccttgctca cactttttgc
    12061 gcaaagttgt tgcgttttgc gtagctgctt ccgcatttgc ctgtcattca ttgcaataaa
    12121 gacgattttc aggaagcgtc ctcgcagact ccgtttttgc cactcggctt tgctttcatc
    12181 acgttgtata tctgcctgac tatctacagc ggaggtgatg atgaaagtat ggatggcgat
    12241 attaataagt atcttgtgct ggcaatcatc tgtgtgggcg gtctgtccgg cctggtcgcc
    12301 agccagagca caggaagaaa tttcccgcct gcaacagcaa ataaaacagt gggacgatga
    12361 ctactggaag gaagggaaaa gtgaggtgga agacggtgtt tacgatcagt taagcgcccg
    12421 tcttacgcag tggcaacgct gctttgggag cgagccccgc gacgtgatga tgccgccttt
    12481 aaatggtgcg gttatgcatc cggttgccca tacgggtgta cgcaaaatgg tggataaaaa
    12541 cgcattaagt ctgtggatgc gagaacgtag cgatctttgg gtgcagccaa aagttgatag
    12601 cgttgcggta accctggttt atcgggacgg gaaactgaac aaagcaatca gtcgcggtaa
    12661 tggcctgaaa ggcgaggact ggacgcagaa agttagctta atttccgctg tgccgcaaac
    12721 cgttagcggg cctttagcca acagtacgct tcagggggaa atatttctcc agcgcgaggg
    12781 gcatatccaa caacaaatgg ggggaataaa tgcccgcgca aaagttgctg gcctgatgat
    12841 gcgccagggg aatagcgaca cgctaaactc actggctgtt tttgtctggg catggccgga
    12901 tggaccgcag ttaatgaccg atcgtttaaa ggagctggct accgcaggtt ttactctaac
    12961 gcagaggtat acccgtgcgg ttaaaaatgc tgatgaagtt gcgcgcgtac gcaatgagtg
    13021 gtggaaagcg aaattaccct tcgtcaccga tggcgtggtt gtgcgagcgg ctaaggaacc
    13081 cgaatcccgc cattggctac caggccaggc agaatggttg gtggcctgga aatatcaacc
    13141 tgtagctcag gttgccgaag tgaaggcgat tcagtttgcg gtgggtaaga gcggtaaaat
    13201 atcggtggtt gcgtcactcg cacctgtcat gctggatgat aaaaaagtcc agcgggtgaa
    13261 tattggttcc gtcaggcgct ggcaggagtg ggatattgcg cctggtgatc agatcctcgt
    13321 cagccttgcc ggtcagggta ttcctcgcat tgatgatgtg gtctggcgcg gtgcagaacg
    13381 tacaaaaccg acaccaccag aaaaccgctt taactcgttg acctgctact ttgcttctga
    13441 tgtttgtcag gaacagttca tttcacgctt agtctggctg gggtcaaaac aggttcttgg
    13501 gctggatggc attggtgagg ccggctggcg cgcgctgcat cagactcatc gctttgagca
    13561 tatcttttcc tggcttttat taacgccaga gcaattacag aacacgccgg ggatcgcgaa
    13621 aagtaaaagt gcgcagttat ggcatcagtt taatctggct cgtaagcagc cttttactcg
    13681 ctgggtgatg gcaatgggaa taccgttaac ccgggcggcg cttaatgcca gtgatgaacg
    13741 gtcctggtcg caacttttat ttagcacgga gcagttctgg cagcggctgc cggggactgg
    13801 atctgggcgc gccagacagg ttattgaatg gaaggaaaat gcgcaaatca agaagctggg
    13861 cagttggctg gctgcccagc agatcacagg ttttgaacct tagtgacctt cgtggctgta
    13921 gtaaaagacc ggtaatccca gcttaagacg tagcgccagt aagcgggcaa aaaagccgaa
    13981 taccagggtg gagataatca ccacatcatg gttagaaaca tagtgttgca gcgcgatgta
    14041 cagcacggcg gaggcaaatg agacaccggc atacaactct ttctggaata ccagtgggat
    14101 gcgtttacag aacatatcgc gcaaaacgcc gccaaacacg ccggtggtta ccgccgcgac
    14161 aacggcaata attgggccgt gccccatatc cagtgcaacc tgtgcgccga tgatagaaaa
    14221 gaccaccagt ccgagcgcat cgagcaccag aaacactttg cgcaggtaag gcattacagg
    14281 agcaacgata gtggtgagta ctgcggcggt agcgacgata atcacatatt ccgggtgttt
    14341 gacccagccg agcggatagt ggcccagcag aatatcgcgc actgaccctc cgccaattgc
    14401 ggtggcgatc gcaataataa ttacgccaaa tgtatccatg cgccgtcgtc cggccgccag
    14461 cgcccccgtc atggcttcgg cagtaatgcc aaccaaatac aaaatgtgta acagcatggt
    14521 tctctcccta aaaatctgtc tggcagggta gcgatttgtg cgtattgtca cgattgagat
    14581 tttttaaggt gagaggaaat tgataacttg tagttatgtg aaatcgctat tttctgtagt
    14641 gtagatgcat tcttctgact tcttgtctga gaaaatgcat aagaaaaaat aggttaaaat
    14701 tgaggggtgg aaaagaaagg tgcaatgttt agccattgtg ttcactgcac ctggactttg
    14761 gcttacgcat cgggtatctc aattaagaaa aagagttttt agtttctgct atttcttctc
    14821 tgtaattttt acactgtttt ccaactgatt gatgctgacc tgcggggtcg gcaactcctc
    14881 aggcatggtt ccgccaagtt cctgaatggt ttgcctcacc ttgctaccca cgtcaaagtg
    14941 tgtggtatta gcttgctgtt ttgaatttac gccatcccgc ttgagttttt cttctgtttg
    15001 ggtagctcga aagagattag ccgccagttc tgttgaaccc atatgatcca ggatcttctg
    15061 actctttttc agccccttcc gctgatggat ggctttctga tctaatccac catacagccc
    15121 ccggtaacca tgattctgaa atatggcgaa atcgatagct gtcgctaccc cagcttgctg
    15181 cgctgcctca actaattgtt tgttatgttc tttcaactca tttctcagaa aaagacgctt
    15241 ttcgtcttca cgaagttgcc tgaaagcttc atcgtcagcc agctcctgcc gtcgggtctg
    15301 gatagcaaaa taagtttgcc ctgccgcaat aaccggtttc gcagggtcgc cgttttgtac
    15361 taccaggtaa caggcatagc gagagagatg aacatcttca agttctcgtt gagcccctga
    15421 acctaataca accattttgg tggtttcaac gaaatggtca gatgcagctt gattgctggc
    15481 ttcgcacgct tgagtcgcgc gggccagtac cttttgaaaa ttgcgccagt cccgataatc
    15541 cagtaacggt gccaattctc tcgctgacca aaattcttgc ccttcagtgc cataatgccg
    15601 tatttcttca aaaggttgat gatgttcgtt cattgcccac tccatgtgat atcgattgca
    15661 aagaaagtaa gtctataact gtatttatat acagttgtga gtatctcacg ttctcactgc
    15721 gctgcgcaaa aaaacatcgc aaaatctgga gcaggtacgc aaaacagaat tgaatgttgc
    15781 ggggaagaaa aatgccgctc tgtttgagtg ggcggcattt gtgaaggctg aaaggtaaga
    15841 attagtgtta cgagttactc aatattctga atctgctcgc gcatctgctc aatcaacact
    15901 ttcagctcga tggcagaatt cgtcacttcg gcattgatag atttcgacgc aagagtgttc
    15961 gactcgcggt tgaactcttg catcataaag tccagacgac gaccaacggc ttcttttttc
    16021 ttcagaatgt tgtaggtctc tttgacatgc gcttccaggc ggtccagttc ttcggcaaca
    16081 tcaattcgtt gtgccagcag aaccaactcc tgctccaggc ggttgttttc cagttgcact
    16141 tgagcatctt ccagcttcgc gaccagacgc tcacgctgcc attgcaggat ttccggcata
    16201 tgggcgcgga ctttgaccac ttcggcggtg acgccttcca gacgctgctc gatcaacgct
    16261 ttcagcgcct gaccttcggt ttcacgtgcg acgataaagt cgtccagggt accatccaac
    16321 gccgcgagaa tttcagcggc aatcgcgtcg agatcctgct cctgggctgc cattacgccc
    16381 ggccagcgca gaatatcaac cgggttgatt tccccttcgt cactctgcat ttttacccag
    16441 ttcgcggcag ttaccagctg tttagccagt ttttcgttga ggatcagctc accttgcgcg
    16501 ctaacatctg gctcatagcg cagggtacat tccactttac cgcgcgtcag gcgagaacga
    16561 atacgctcgc gaacgacagg ttcaaggcta cggaactgct ccggcagacg aaagtaagtt
    16621 tccagataac gctggtttac cgagcgcatt tcccaggttg cgctccccca ttcacccttg
    16681 atttcacgcc gggcgtaggc ggtcatactg cggatcatag acgttcctgt ttataaaagg
    16741 agaggtggaa ggattatagc catcgatgcc ttgtcaggat aggaataacc tccggaagtc
    16801 cgtataatgc gcagccacat ttgtttcaag ccggagattt caatatgcgt ccagcaggcc
    16861 gtagcaataa tcaggtgcgt cccgttaccc tgactcgtaa ctatacaaaa catgcagaag
    16921 gctcggtgct ggtcgaattt ggcgatacca aagtgttgtg taccgcctct attgaagaag
    16981 gcgtgccgcg cttcctgaaa agtcagggcc agggctggat caccgcagag tacggtatgc
    17041 tgccgcgttc tacccacacc cgtaacgctc gtgaagcggc gaaaggtaag cagggtggac
    17101 gcacaatgga aatccagcgt ctgatcgccc gtgcgcttcg cgcggcagta gatttgaaag
    17161 cgctgggtga gttcaccatt acgctggact gcgacgtgct tcaggctgat ggtggcactc
    17221 gtaccgcttc gattacgggt gcctgcgtgg cgctggcaga tgcgctacag aagctggtgg
    17281 aaaacggtaa gctgaaaacc aatccgatga aagggatggt agccgcggtt tctgtcggga
    17341 ttgtgaacgg cgaagcggtt tgcgatctgg aatacgttga agactctgcc gcagagaccg
    17401 acatgaacgt agtgatgacc gaagacgggc gcatcattga agtgcagggg acggcagaag
    17461 gcgaaccgtt cacccacgaa gaattactca ccttgttggc tctggcccga gggggaatcg
    17521 aatccattgt agcgacgcag aaggcggcgc tggcaaactg atttttaagg cgactgatga
    17581 gtcgcctttt tttgtctgta gaaaagtaag atgaggagcg aaggcatgaa accatatcag
    17641 cgccagttta ttgaatttgc gcttagcaag caggtgttaa agtttggcga gtttacgctg
    17701 aaatccgggc gtaaaagccc ctatttcttc aacgccgggc tgtttaatac cgggcgcgac
    17761 ctggcactgt taggccgttt ttacgctgaa gcattggtgg attccggcat tgagttcgat
    17821 ctgctgtttg gccctgctta caaagggatc ccgattgcca ccacaaccgc cgtggcgctg
    17881 gcggagcatc atgacctgga cctgccgtac tgctttaacc gcaaagaggc aaaaaaccac
    17941 ggtgaaggcg gcaatctggt tggtagcgcg ttacaaggac gcgtaatgct ggtagatgat
    18001 gtgatcaccg ccggaacggc gattcgtgag tcgatggaaa ttattcaggc caatggcgcg
    18061 acgcttgctg gcgtgttgat ttcgctcgat cgtcaggaac gggggcgcag cgagatttcg
    18121 gcgattcagg aagttgagcg tgattacaac tgcaaagtga tctctatcat caccctgaaa
    18181 gacctgattg cttacctgga agagaagccg gaaatggcgg aacatctggc ggcggttaag
    18241 gcctatcgcg aagagtttgg cgtttaaaga aactcgccgg atgaaaagtc atccggcgtc
    18301 atattactgc aactgcgcgg caattagcgg ccagcgggcg tcaaaatcat ccgtcgggcg
    18361 gtatttaaat tcgctgcgga caaaacgtga cagcatacct tcacagaagg ccaggatctg
    18421 gcttgccagc agggtttcat cggtggtgta accttcaccc tcacgcattc tcttttcacg
    18481 caatacctgg cgcagctgcg cttcaatacg ctcgaacagc tggttgatgc gcccttgcag
    18541 gcgatcctgt tcaaacatta gcgcatgacc agtgaggatg cgggtcaggc caggattacg
    18601 ctcaccaaaa ccgagaagca gcaacacaat cagacgcagg cgcgctgtgg tgtctttctc
    18661 atctttcaga atcaggttga tgcgagtaat caggctatct tcgataaact caatcagact
    18721 atcgaacatg cgggtcttac tggggaagtg gcgatacagt gccgcttcgg aaacgccgac
    18781 agaggcggcc agttttgccg tcgtgatacg ttggcttcca tcgctggatt ccagcatcag
    18841 cgccagagac tgaagtattt cctcgcgacg gttccttttc gcagtttgtt tttctgccat
    18901 gttacaaaat acccctgaaa ataagcactt gccaggcggc acccacgcta tgaccgcaaa
    18961 cgaaatgttt gcggctatgt tatgacgtta ttcggatgcg tatgtgttac tgacgaccag
    19021 agtgaccaaa gccgccttca ccgcggtcgg tggcgtcgaa atcttccacc agattaaatt
    19081 cagcctgtac taccggaaca aaaatcatct gggcgatgcg ttcgccaggt tgaatggtga
    19141 agctgtcctg accacggttc cacacggaaa tcatcaactg gccctgatag tcagaatcga
    19201 tcaatcctac caggttacca agcacgatac cgtgcttatg tcccaatccg gagcgcggca
    19261 gcatcattgc cgccagtgaa ggatcggcaa tatgaatcgc cagcccggtc ggaaccagcg
    19321 tagtgtcacc cggagccagt tctacggcgt cgtcgagaca ggcacgcagg tcaagtccgg
    19381 cagagccaga ggtggcataa gtcgggagcg gaaattcctt cccaacgcgc gggtccagaa
    19441 tcttaacgtc gatttttttc atcataacgg gtcacgatct cgtcgagtaa taattggcca
    19501 aggagctctt tgcgctcaag cggtaagact ttatctccgt cctgccagaa aaggtgtaat
    19561 gcgttgttgt cgctgttaaa tccttgagtt ggctgggaaa catcgttcgc gcagatcaga
    19621 tcaaggtttt tacggatacg tttttgccgg gcgtattctt ccacattatt tgtttcggcg
    19681 gcaaatccaa cgacgtaggg tcgatggtct tttagtgcgg caacgcctgc gacgatatcg
    19741 gggtttttaa ccatttttat tgttaattca tcaccctgcg tggcctgctt tttgattttc
    19801 tctggggcca cggtagctgc gcgataatcc gccacggcgg cgcagccgat aaaaatattt
    19861 tgctgctgta cagaagcatt cacggcgact tccatttcca gcgcggtcat cacatcaaca
    19921 cgtttaacaa acggtggcgt cggtagcgaa accgggcctg ataccagcgt gacgttcgcg
    19981 ccacgacggg cagcggcggc ggcgatagca aaacccatct tgccggagct gtgattagag
    20041 atataacgca ccggatcgag cggttcacgc gtcgggccgg cggtaatcat aatgttcaga
    20101 tgtttcaggt cgttgacggg cgaaaaatgc gctaccgcca tatccacaat ggttaacgga
    20161 tcgagcattc gcccaggacc gatatcacca caagcctgac tgccactgtc tggcccccag
    20221 atgagcaaac cacgggaagc aagcacctct aaattatgct gcgtggcagc ggcacggtac
    20281 atctgctggt tcatggcggg gagcacggct acaggcgcag gtgtagccag acaaatcgtc
    20341 gataccaggt cattcgccat tccggcagca acacgggcaa tcaaatctgc cgtggcaggg
    20401 gcgagaatca ctaaatcagc ccatttaccc agctcaatat ggcccatagc ggcttcggct
    20461 gccgggtcca gcagactgtc ggaaacggga taaccagaaa ccgcctgcaa gctaagtggg
    20521 gtgataaagg cttttgccgc ttcggtcatg gctacgcgga cgtcggcccc gcgatcgcgc
    20581 aaacgacgca ccagttcagg ggttttatag gcagcaatac cgccgctaac gccgagaacg
    20641 atttttttac cggccaggct catgatgatt tttcctgtag ggtgacacca gaagttggcg
    20701 attttatcac aatacttttg ttgtcgtgcc ttcacctgag attcactttg cgaggcgctt
    20761 tccaggattg aaaactggcc gtcgatttaa cgaaacggct atgacaggat gcgagcacca
    20821 caaaggaggt gaaggtgaaa aacaatgcac agctgttgat gccgcgcgaa aaaatgctga
    20881 agtttggtat tagcgcctta acggatgtcg agctgctggc gctatttctg cgtaccggaa
    20941 cgcgcggtaa agatgtatta accctggcaa aagagatgct ggagaatttc ggctctcttt
    21001 atggc
//
LOCUS       DAKISA010000079        20775 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.079, whole genome shotgun sequence.
ACCESSION   DAKISA010000079 DAKISA010000000
VERSION     DAKISA010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20775
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.079"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(31..2478)
                     /gene="glgP"
                     /locus_tag="OVW45_002924"
     CDS             complement(31..2478)
                     /gene="glgP"
                     /locus_tag="OVW45_002924"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCV5527323.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2497..3930)
                     /gene="glgA"
                     /locus_tag="OVW45_002925"
     CDS             complement(2497..3930)
                     /gene="glgA"
                     /locus_tag="OVW45_002925"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCV5527324.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDNNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3930..5225)
                     /gene="glgC"
                     /locus_tag="OVW45_002926"
     CDS             complement(3930..5225)
                     /gene="glgC"
                     /locus_tag="OVW45_002926"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCV5527325.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5243..7216)
                     /gene="glgX"
                     /locus_tag="OVW45_002927"
     CDS             complement(5243..7216)
                     /gene="glgX"
                     /locus_tag="OVW45_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCV5527326.1"
                     /translation="MTRLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYACACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIALGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(7213..9399)
                     /gene="glgB"
                     /locus_tag="OVW45_002928"
     CDS             complement(7213..9399)
                     /gene="glgB"
                     /locus_tag="OVW45_002928"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCV5527327.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9672..10775)
                     /gene="asd"
                     /locus_tag="OVW45_002929"
     CDS             complement(9672..10775)
                     /gene="asd"
                     /locus_tag="OVW45_002929"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCV5527328.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10967..11560
                     /gene="yhgN"
                     /locus_tag="OVW45_002930"
     CDS             10967..11560
                     /gene="yhgN"
                     /locus_tag="OVW45_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCV5527329.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11606..12901)
                     /locus_tag="OVW45_002931"
     CDS             complement(11606..12901)
                     /locus_tag="OVW45_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCV5527330.1"
                     /translation="MDADSSDSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASEYKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAYTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(12808..14778)
                     /locus_tag="OVW45_002932"
     CDS             complement(12808..14778)
                     /locus_tag="OVW45_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HCV5527331.1"
                     /translation="MEKHGAELLLQRMLSNTSATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLRGGVDCLLISPERLANQDFLETANTPVLGTTATANNRVVEDIRQQLGDI
                     VIQRGTLARESLALDALVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLR
                     TNGISAFAYYSGVTCEGAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFV
                     IHYQMPGSIVGYYQQVGRAGRAIDSAVGILLCGGEDRAIHKFFRESAFPAEAQIHEIL
                     NVLSENDGLTLRGIEQRTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSLDH
                     ERINHLMNQRKNELADVESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPD
                     IDSGLLHAANLFIKHADLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRVLSRWGDS
                     GWGKQVAQEKKTGRFSDELVEACAEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFARR
                     LAAKLGLPFIDAIEKVVDNPPQKMQQNRFHQCQNLDGAFVITPPLMPGPALLVDDIVD
                     SAWTLTVLTALLRQAGCPTVYPLALASTSVKN"
     gene            complement(14919..16259)
                     /gene="gntU"
                     /locus_tag="OVW45_002933"
     CDS             complement(14919..16259)
                     /gene="gntU"
                     /locus_tag="OVW45_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCV5527332.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTDTNLVKLIIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNNSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(16263..16790)
                     /gene="gntK"
                     /locus_tag="OVW45_002934"
     CDS             complement(16263..16790)
                     /gene="gntK"
                     /locus_tag="OVW45_002934"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCV5527333.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(16929..17924)
                     /gene="gntR"
                     /locus_tag="OVW45_002935"
     CDS             complement(16929..17924)
                     /gene="gntR"
                     /locus_tag="OVW45_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCV5527334.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(18148..18843)
                     /gene="yhhW"
                     /locus_tag="OVW45_002936"
     CDS             complement(18148..18843)
                     /gene="yhhW"
                     /locus_tag="OVW45_002936"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCV5527335.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(18966..20003)
                     /locus_tag="OVW45_002937"
     CDS             complement(18966..20003)
                     /locus_tag="OVW45_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCV5527336.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDICSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            20337..>20775
                     /gene="yhhY"
                     /locus_tag="OVW45_002938"
     CDS             20337..>20775
                     /gene="yhhY"
                     /locus_tag="OVW45_002938"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCV5527337.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQHPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYA"
BASE COUNT         5132 a         5678 c         5100 g         4865 t
ORIGIN      
        1 cctttggccc cgttctattt attggtgaac ttacaatctc accggatcga tatgccagat
       61 atgatcggcg tactctttga tagtacggtc agaagagaag tagcccatat tggcaatgtt
      121 cagcatcgct tttgcggtcc actcttcctg aagctcgtag agttcgtcga ctttatcctg
      181 acaatcgaca tagctgcgat aatccgccag aacctgatag tgatcgccga agttgatcag
      241 cgaatcaacc agatcgcgat agcgacccgg atcttccgga ctgaatacac cgctgccgat
      301 ttgcgtcagc acctgatgca gctcctcatc tttctcgtag tattcacgcg gtttgtagcc
      361 ctgacgacgc agttcttcca cttcttccgc tgtgttacca aaaataaaga tattgtcagc
      421 accgacatga tccagcatct cgacattcgc accgtccaac gtaccgatag tcagcgcacc
      481 gttaagcgca aacttcatgt tactggtgcc ggaagcttcc gtccctgcca gcgaaatctg
      541 ttcagacaga tctgccgccg gaatgatcaa ctgcgccagg ctaacgctgt agttcgggat
      601 gaacacgact ttcagcttat cgccaatctg cggatcgttg ttgatcactt tcgctacgtc
      661 attgatcaaa tgaataatgt gcttcgccat gtaataggcc gaagccgcct taccgccaaa
      721 aatattcacg cgcggtaccc acttcgcatc cgggtcggcc ttgatgcggt tatagcgggt
      781 aatcacatgc aacacattca tcaattgacg tttgtattcg tgaatacgtt tgatttgtac
      841 atcgaacaac gcctttggat tcaccaccac attcagctgc tgggcgatat actctgccag
      901 acgctttttg ttctccagct tcgcctgatg cacagcgtga ttaaccattg ggaaatcaca
      961 gtgttgttgc agctcattaa gcaggctaag gtcggtgcgc cagttacggc ccaggtgttc
     1021 gtccagcacg gctgaaagcg atgggttcgc taccgccagc cagcgacgcg gcgtcacacc
     1081 gttggtgacg ttggtgaaac gacccgggaa gattttcgca aagtcggcaa acaacgattg
     1141 caccatcaga ttagagtgca gttccgatac accgttaact ttgtggctca caacaaccgc
     1201 cagccaggcc atacgcacac gacgaccgtt ggattcatca atgatcgacg cccgtcccag
     1261 cagatcggta tcgttcggat actgttcctg caaggttttc aggaaatagt cgttgatttc
     1321 aaagatgatc tgcaggtgac gcggcagaat tttacccagc atatcaaccg gccaggtttc
     1381 cagcgcctcg ctcatcagcg tgtggttagt gtaggagaag acctgacaac acacctcaaa
     1441 cgcgtcgtcc cagctaaatt ggtgctcatc gatcagcaga cgcatcatct caggaatcga
     1501 cagtaccgga tgggtatcat tgagatgaat cgcgatttta tccgccaggt tatcgtaggt
     1561 tttatgcaac tgataatggc ggcttaaaat gtcctgaatg gtcgaggaaa ccaggaagta
     1621 ttcctgacgc aggcgcagct cacgcccgga gtaggtggag tcatccggat acagtacgcg
     1681 agatacgttc tcggagtggt ttttatcttc cactgccgcg aagtagtcac cctggttgaa
     1741 tttaccgagg ttaatttcgc tactggcttg cgcactccac aaacgcagcg tgttggtcgc
     1801 gtcggtgtcg taaccaggga ttatctgatc gtaagcgact cccagaatct cttcggtttc
     1861 aatccagcgc gtttttttac cttcctgctg aatgcgaccg ccaaaacgga ctttatagcg
     1921 cgtgttgtgg cgtttgaatt cccacgggtt accgtattcc agccagtagt ctggcgactc
     1981 tttctggcta ccgttaacga tgttctgctt gaacataccg tagtcatagc ggatgccgta
     2041 accgcgcccc ggcaacccta acgtcgccag agaatcaagg aagcaagccg ccagacgtcc
     2101 caggccaccg ttaccgaggc ctgggtcatt ttcttcatca atcagctctt cgagatttaa
     2161 ccccatcgct tccagtgcgc cctgtacatc ttcgtaaatt cctagcgaca acatggcgtt
     2221 ggagagcgta cggccaatca aaaactccat cgacaggtag taaacctgac gtgtttcttg
     2281 cgacaactgg gcacggtttg aacgtaacca gcgctccacg agacgatcgc gcacagcaaa
     2341 taacgttgcg ttcagccatt catgtttatt ggcgacgacc gggtcctttc caatcgtaaa
     2401 catcagcttg taagcgatag agtgcttaag agcttctacg ctaagcgtgg gcgatgaata
     2461 tgtaaacgga gcattcatat aggcgtttcc tgaaaactat ttcaagcgat agtaaagctc
     2521 acggtacgac ttcgccgcga cctgccagct aaaatccatt gccatagcct gacgttgcac
     2581 aaaccgccac agtgaaggac gggaccacag tacaaaagca cgccgaatag cccgtaacag
     2641 cgaccaggca ttattatctt caaagacaaa cccactggcg acgccatctg caaggttctc
     2701 aagagaacag tcagaaaccg tatcagcaag cccaccggtg cgccgcacta acggcagcgt
     2761 accgtacttc aatccataaa gttgcgttaa gccgcacggt tcaaaacggc tgggcaccag
     2821 aatgacgtcc gcgccgccca taatgcgatg cgaaaatgct tcgtgatagc caatctgaac
     2881 gcccacctga ccggggtatt ccgctgccgc cgcaaggaaa ccttcctgca gcaccggatc
     2941 gcccgcgccg agtagcgcca gctgcccgcc ctgctccaga agacccggta aggcttccag
     3001 caccaggtcg agacctttct ggctggtcag acggctcacc actgcaaaaa gcggcacttt
     3061 atcgtcaacc ttaagcccca ttgcgatttg taactggcgc ttattttccg ctttatcttc
     3121 caacgtatcg cgggtgtagc gcgaggccaa cagtaagtcc gtctctggac tccagatttt
     3181 ctcgtccacg ccgttcagta cgccggaaag acgcccttca cgatgacgct gttgcaacag
     3241 accttccata ccgtaggcaa actgcggttc ggtgatctcg cgagcgtagg ttggactgac
     3301 cgccgtaatg tgatcggcat agtacagacc ggccttcagg aaagagattt gtccgttgaa
     3361 ttccagccca tgaatattaa agaatgacca tggcaattgg atgtcattca tgtgatgtgc
     3421 ataaaacatg ccttgatagg ccaggttgtg cacagtaaac accgacttcg caggacgccc
     3481 gcgcgccgcc agatacgcag gcgcaaggcc tgcatgccag tcgtgcgcat gcaccacatc
     3541 aggacgccag aatgggtcaa gcccgctggc catttctgcc ccaacccacc ccagaagcgc
     3601 aaaacgcaat acgttgtcgg tataggcaaa taagttggta tcgtgatacg ggcttcccgg
     3661 acgatcatag agatgcggcg cgtcaatcag gtatatgcca accccgttgt aatgaccgaa
     3721 caacagcgtg atatgtccgg cgaaggtatc acgacgggat actacctgcg catcggtcac
     3781 gccacggcga atatcgggaa atgcaggcaa cagtacgcga gcgtcaacgc cgtctgcgat
     3841 ttgtgctgcg ggtaatgccc caataacatc agccagaccg ccggttttaa gcagcgggaa
     3901 catctctgaa catacatgta aaacctgcat tatcgctcct gtttatgccc taacttccgt
     3961 agcatttcgc gcgttaccag cacgatgcct tcttctgaac gatagaaacg acgtgcatct
     4021 tcctctgcgt tttcaccaat caccatgcct tccggaataa cacaagcacg atcgatgacg
     4081 cagcggcgca gacggcacga gcgacctacc catacttccg gtaacaatac ggcggaatca
     4141 atgttgcaga atgaattcac gcgaacgcgc gagaacagaa cggactgcac caccaccgaa
     4201 ccggagatca cacagccgcc ggataccagt gagttaaggg tcatcccgtg gctaccggag
     4261 cgatcctgca cgaatttcgc tggcggtaat gattcattgt aggtgcgaat tggccaattg
     4321 cgatcgtaca tatccagttc cggcaccaca gaggccagat cgaggttcgc tttccagtaa
     4381 gcttccagcg tacccacatc gcgccagtac ggctcggcat ccgggtcgga ttgtacgcaa
     4441 gagagcggga acgggtgcgc ataggccaga ccggcttcgg tgatcttggg aatcaaatct
     4501 ttgccaaagt cgtggctgga gttctcatca cgatcgtctt cttccagcag ttcatacaga
     4561 tagtcggcgt caaagacgta gatacccata ctcgccagag atttgctcgg atcgttcggc
     4621 attgacggtg ggttagcagg tttttcaacg aattcgataa ttttatcgtt ctcatcaacc
     4681 gccataacgc caaatgcgga ggcttcttca atcggtactg gcatacaagc aacggtgcaa
     4741 cgtgcgcctt tttcgacgtg atcgataagc atacgcgagt agtcttgctt gtagatatgg
     4801 tcgcccgcca ggatcaccac gtattccgct ttataacggc ggataatgtc gaggttttgg
     4861 gtgaccgcat ctgcggtgcc gcgataccag ttttcccctt tcattctctg ctgtgctggc
     4921 agcagatcga caaactcgtt catttcttca ttgaagaatg accagccgcg ctgaatgtgc
     4981 tgcaccagag tgtgggactg gtactgggtg atcacgccca tacgacggat cccagagttg
     5041 atgcagttag acagcgcaaa gtcaataatg cggaacttac cgccgaagtg tacggccggt
     5101 tttgctcgct tattggttaa atccttcagg cgggtaccac gtcctcccgc cagtatcagg
     5161 gcaacagatt tcaatggcag ctggcgcgcc aacattaagt gatcgttctt ctctaaacta
     5221 accatgacta actccttttt tatcatctct ggaacacaca caatccgtgt gcaggtccct
     5281 gccagacagc cgtaatcact gggttatcct ctccagcgaa tgggggaatg gcgtgccact
     5341 ccccagcagg taaaacaatc tctgttacct caagcgtggc gttaattgcg atcaaaaagc
     5401 gatccgagag cagaatttgc agctgtttcg gcccgttttg ccactcatcc gtgcttaaag
     5461 gttgagcata tcgatttagc caacggacat tgccgtcgcc ttcttcccac cagcgattct
     5521 ccaccaaagc gggaatgcgc ttgcgcagat ggattaacgc ggcggtaaat gcggttaaac
     5581 cactgcttgc ctgcgaccag tccaaccagg ttaattggtt atcctgacag taggcattgt
     5641 tattgccatg ctggctgtga ccgtgttcgt caccggccag taacatcggc gtaccctggg
     5701 agagcaacaa cgttgttaac agggcgtgaa tactgtcgcg ccgccgttca accaggtcaa
     5761 gagaaccgcc taacccttct ttaccatgat tgttactgta attgttgttg gtcccgtcgc
     5821 gattttcttc tccgtttgct tcattgtgtt tatggttgaa gcaaacgcag tcgcgaagcg
     5881 taaaaccgtc atgcgcagtg acgagattaa tcgcggcact cggcagacga ccattacgtt
     5941 taaaaacatc gctggaggca gcaaaacgcc cggcaaacgc ccccagaggc aaatcataat
     6001 gtagccagaa acgacgggca gcatcgcgga aatgatcgtt ccactcggca aacagcggcg
     6061 ggaaatttcc cacctgataa ccaccaaggg cgatatccca cggttcagca attaacttca
     6121 cctgcgagag caccgggcag ttctggatag cggtaaacaa cggcgcatcc tgacggaact
     6181 ctggcgtacg gcccatgact gccgccagat caaagcggaa accatcgacg tgacaggttt
     6241 ctacccaata acgcaggcag gcgcaggcat aatccaccac cgccggatga ctcaaattga
     6301 gcgtgttgcc acaaccggtc cagttgtgat aatcgccgtc ttctcttatc caataatagc
     6361 tacggttgtc gatcccacgc agcgagaata acgggccgtc gaggtccagt tccgcactat
     6421 ggttgagcac gatatcaaga atgacttcga tacccgcttt atgcagtgct ttgattgcat
     6481 cgcgaaactc atgcagcgcc gtttctggcg agcaggcata cgccggatgc agcgcaaaca
     6541 tcgccaccgg gttgtaaccc cagtagttac ttagccccat gcgttgcaga cgtggttcac
     6601 tggcaaactg cgccaccggc agcagttcca gcgcggtaat gcccaactgt ttcaaatagt
     6661 tgatcatcac cggatgcccg agggctttat aagtgccacg gatctcgacc gggatctccg
     6721 ggtgcaggta cgttaatcct ttaacatggg cttcataaat gatggtgctg ccccacggcg
     6781 tgcgcggcgg ggcatcatct tcccagtcat agtgatcaac caccactacg catttcggcg
     6841 caatggcggc gttgtcgcga tagtcaggtt cattatgacc ggcgtgcagc agcgggttat
     6901 ctttgaattc cccttcaatt tgccgcgcgc aaggatcaat caacaacttc gccgggttaa
     6961 agcgatgccc ctcggcgggt tgccaggggc catgaacgcg ataaccataa cgcaaacccg
     7021 ggcgcgcatc cggcagataa ccgtgccaaa tgtcgccact gtgccctggc aagtcatagc
     7081 gatgttcctg gccattggcg tcaaagacac acagctctac ccgctcggca tgagcggaga
     7141 aaagtgtgaa gttgacgccc tgaccgtcgt aatgcgcgcc gaggggagcg ggtttgccaa
     7201 tggcgagtcg tgtcattctg cctcccgaac cagccagata gtggccagcg gtggtagtgt
     7261 caggcttagt gaatgctgac gaccgtggct ggcaatctca tcgctgtgta ccgtgccgcc
     7321 attgcctgca ttactgccgt gatagtgcat ggaatcggta ttgaggattt cacgccattt
     7381 gcctggctga ttaatgccga agcgataatc atgacgcggc accggcgtaa agttactggc
     7441 aacgataatt tcgttaccct ctttatcgcg acgcacaaag atcagcaccg agcgttcttt
     7501 gtcatccacc accagccatt caaagccgta cgggtcaaaa tccagttcat gcattgcttt
     7561 atggtggcgg taggtgaggt tcagatcgcg caccagacgc tggacaccgt ggtgccagtt
     7621 atcgccgcct tccaacagat gccagtcgag gctggcgtca tggttccact cacggccttg
     7681 ggcaaattcg ttccccatga acagcagttt cttgcccggg aatgcccaca tccagccata
     7741 gtaggcgcgc aggttagcga atttctgcca tgcgtcgccc ggcatgcggt cgagaatcga
     7801 ttttttaccg tggaccactt catcatgcga caacggcagg acgaagtttt cagtgtagtt
     7861 gtagagaatc ccgaaggtga gtttatcgtg atgatactga cgataaaccg ggtcgagctt
     7921 catgtagtcc agggtgtcat gcatccagcc gaggttccac ttgtaccaga agcccagacc
     7981 gcccatatcc tgcggacgag aaacgccagg gaaatcggta gactcctcag ccattgtcac
     8041 cgcaccggaa acctgctcac caagaatacg gttggtatta cgcaagaatt caatcgcttc
     8101 aagattctcg cgcccgccaa attcgttcgg gatccactcc ccctctttac ggctgtagtc
     8161 gcgataaatc attgacgcca ccgcatcgac gcgcagcgca tcaataccaa aacgttcaat
     8221 ccagtaaagc gcgttaccga cgaggaagtt actgacttca cggcgaccat agttgtagat
     8281 cagcgtattc cagtcctgat gatagccttc gcgcggatcg ctgtgttcat acaagttcgt
     8341 gccatcaaat tcggcaagcg caaagtcatc ggtcgggaag tggcctggca cccagtcgag
     8401 aatcacgttc agacccgctg cgtgtgcggc atcaatgaaa taacggaagt cgtcgcgagt
     8461 accaaaacgg cgggttggtg catacaggcc ggttggctga taaccccaac tgccatcgaa
     8521 gggatgctcg ttaatgggca gtagttcgag gtgggtaaag cccatccatt tagcataagg
     8581 caccagttga tcggccagct cgcggtagct caaccagaaa ttgttatcgg tgtggcgacg
     8641 ccaggagccc aggtgaactt cataaataga gattggcgca tcaaactgat tcgctttttt
     8701 gcgctcttca gtctgtacaa ccttttccgg cagcccgcaa ataagagacg cggtttccgg
     8761 gcgcatttgc gcttcgaagg cataagggtc ggacttcaga cgcaagttgc cattggcatc
     8821 aatcatctcg tatttataga gctgaccgtt atgcgcccca gggataaaca gttcccaaat
     8881 gccgctctct ttacgcaggc gcatcgggtg acggcgaccg tcccagtagt tgaattgccc
     8941 aaccaccgag acccgacggg cgtttggagc ccagacagag aaacgcgtac ctgtgacgcc
     9001 atccatagta tctgcatgcg cgcctaaggt ttcatacggg cgcaggtgag taccttcaga
     9061 taatagccag gcatccattt cctggattag cggaccaaaa cggtaaggat catcaatcag
     9121 gttttgctga ccatgccaga caacagccaa ctgatagcgg aaaaaattct tacgtcgcgg
     9181 aatgacgccg ctaaagaatc cccgtgagtc gagacactcc agttttgcga gtttgcgccc
     9241 ggttttcggt tcaatcaccc acacatcggt agcgtcgggt aaaagggcac ggacttccag
     9301 tcccgcggtg gttttatgca ttcccagtac ggaaaaagga tccgcaaaat ggcctgcaat
     9361 tagcgcgtta atcacgtctc tatcgatacg atcggacatg cttgtcttcc tgttttattg
     9421 tgtcacccca tccaattgga tttttgactt ctggttgtga catttttttg acctgaacgg
     9481 cgcagcactc tgtgcatcct ctctgcgtcg tcctcacttc aggtaaggct gtgaatactc
     9541 atgtattcag ccacccttaa agaatagcca atgctctatt taactcccgg taaatcatga
     9601 aacatctgcg cttactcctg tattacgcac taacaggggc ggcatcgcgc cccagattta
     9661 atgaataaag attacgccag ttgacgaagc atccgacgca gcggctccgc ggccccccac
     9721 agcagctggt cgcccacggt aaaggctgac aggaactctg gtcccatatt cagcttacgc
     9781 aggcggccta ccggcgtggt cagcgtgccg gtaacggcag ctggggttag ctcacgcata
     9841 gtgatttccc gatcgttcgg aacgactttc gcccacggat tgtgcgcagc cagcagttct
     9901 tcgacggtcg gaatagacac atcttttttc aatttaatag tgaatgcctg gctgtggcag
     9961 cgcaatgccc cgacacgcac acataaacca tctaccggaa ttacggaaga tgtgttgagg
    10021 atcttgttgg tttccgcctg ccctttccac tcttcgcggc tctgaccgtt atcgagctgt
    10081 ttgtcgatcc acggaatcag gctacccgcc agcggcacgc caaagttatc caccggcagc
    10141 tcaccgctac gggttaaggt tgtgactttg cgttcgatat cgagaatagc agaggacggg
    10201 gtcgcgagtt catctgccac atggccatac agatggccca tctgggttaa taactcacgc
    10261 atatgtcgcg caccaccgcc ggaagcggcc tggtaggttg caacggacac ccaatcaaca
    10321 agatcattgg cgaataaacc acccaacgac atcaacatca ggcttacggt acagttaccg
    10381 ccaacaaaag tcctgatgcc attatttaat ccgtcggtaa tgacgtcctg attgacgggg
    10441 tcaagaatga tgatggcgtc atctttcatg cgcagagacg atgctgcgtc aatccagtaa
    10501 ccttgccatc cgctttcacg aagctttgga tagatttcgt tggtataatc gccgccctga
    10561 caggtcacaa tgatatcgag ggcctttagc gcctccagat caaaggcatc ctgaagtgtg
    10621 ccagtggttc cgccaaaaga cggcgcagcc tggccaagct gagaagtaga aaagaagaca
    10681 gggcgaatgg cgtcgaagtc gcgctcttca accatgcgtt gcatgagaac ggagccgacc
    10741 ataccgcgcc agccgataaa accaacattt ttcataagcg ttttttcctg caaagatgtg
    10801 tgctgtataa atgtgccggt ctcctcttgg cacatctttc accatacaaa aagcagccaa
    10861 agtcgcaagt gaaattaatc aatgatagcg aagccatcag taatgcgact tatcctgctt
    10921 tgttagcacg cagaaagtcc gcggcaatta tcagggaatt tgagttatga atgaaatcat
    10981 ttctgcagca gttttattga tcctgattat ggatccgctc ggaaacctgc ctattttcat
    11041 gtccgtactg aaacatactg aaccgaaaag acggcgggca atcatggtgc gagagttgct
    11101 tattgctctc ctggtgatgc tggtgttcct gtttgcgggt gagaaaattc tggcatttct
    11161 cagcctgcga gcagaaaccg tctccatttc tggcggcatc attctgtttc taatcgccat
    11221 taagatgatt ttccccagcg cttcaggaaa tagcagcggg cttccggcag gtgaagagcc
    11281 atttatcgtg ccgttggcaa ttccgttagt cgccgggccg actattctcg ccacgctgat
    11341 gttgttgtct catcagtacc cgaatcagat gggacatctg gtgattgctc tgctgctggc
    11401 ctggggcggc acctttgtca tcctgctaca gtcttcgcta tttttacgtc tgctgggcga
    11461 gaaaggggtg aacgcacttg aacgcctgat gggattgatt ctggtgatga tggcaaccca
    11521 gatgttcctc gacggcattc gaatgtggat gaaggggtaa ggttcaaaca ggtgctgcac
    11581 acaaccagac gcggcacctg ttttattacc tgaactgata atgaccttcg cccaatcgga
    11641 taacccttcc ctcttcttct gcgcggttca gccacacact aatttgttcg atggttaaac
    11701 cagtacaaga cgccagcctt tcaatactta tcgattcctt tgcaaggatc gccagttccg
    11761 ccacaaagag ttgataaaaa tcgacaggcg tatacgcggg cattactgcg taatttgcgt
    11821 catcttcatg cagcgaaaac agttctggtt gagcgttacg gagtgctctg tcatacatca
    11881 gcccaggaac gtctggcagt ctgatcgtca cattctcagc ctgttcagca ctccatgacg
    11941 ccccattttc tactaatcgt gaattggctg attgcatatc ctgatttggt ttaacctgca
    12001 caggcagcca ctgatgtttt aatgcctcca tcgccccggt tatcgttcca ccggtcattc
    12061 ccgcacgtac aaccattgcg ctttcagcaa ggcaataaat atatttattt cgcgccatcg
    12121 cattgccgac ggtgaaacgt acctctgggt aaaacggcga aatcaacacc aggttgcctg
    12181 ctataagccc ttcacgccat ttcactaacg tactcgtttt taacaagcta tcagctaata
    12241 cgccaacggc agttcccccg gcctccagtg ccgacgccat tgcacattca tcaatacctc
    12301 gcgcaccacc agagataacg caaatcccct gttgggccag tttcgcggcc agttgttggg
    12361 tatagcgcaa atcgtcagtc ggagcatctc gcgagccaac aatcgccata ccttccgctt
    12421 gcagtaatgc tttattcccg cagccaaata aaacgggagg tgcatccgtt cgcaatcggt
    12481 ttttcaagcg aacgggataa tcagcatctc cgcgggttaa gatccataaa ccggcgcgtt
    12541 gccacttatc taccgccaac gccagactat gaccacgcgc cagcaagcca agaatacgct
    12601 cctgagaaat acgcggatcg ctccagcctg taagtttctc ttgcggttgc ggcaccagaa
    12661 gctcggcggg actgatacgt tggtgcttca gccataatgc aaacttcccc cattcactat
    12721 tactaagagg tttatactca ctcgccgccg cgcgagaaaa atcgctggtt agcaggagag
    12781 ttgcttgtgc attggctgaa agattcatca attttttacc gaggtagaag caagggcaag
    12841 aggataaacc gtcgggcaac ctgcctggcg taacagtgct gtcagaactg tcagcgtcca
    12901 tgcagaatcc acgatatcgt caaccagcaa cgccggacca ggcatcaaag gaggggtaat
    12961 aacaaacgcc ccgtcgagat tttgacactg gtggaaacgg ttttgctgca ttttctgcgg
    13021 tggattgtcc acgacttttt caatggcatc aataaaaggt aagccaagtt tcgccgccag
    13081 tcgccgggca aaatcaggaa ccaggtcgag gtgctttaat gaaggaacgc agcataccca
    13141 ggttggttca ggatgcggat tccagcgttg gcgaaccatt tccgcacatg cctccaccag
    13201 ctcatcactg aatcgccccg tttttttctc ctgtgctacc tgctttcccc acccggaatc
    13261 tccccaacga gaaaggactc gccccgtaga tccttgtaaa cccgcaggca agttcccttt
    13321 aaatccgtat tgcgtaaaag ctccagatgc tacctgttta ttgagattta atggcaggtc
    13381 agcgtgttta ataaataaat ttgccgcatg gagtaagccg ctatcaatat cgggcgacaa
    13441 caacggatgc tgaagacaac tgctacattt accacaacgt tcggcacttg gctcatcgag
    13501 tgcgcggcgc agaaattgca ttttgcactc cttggtcgtg atatagcttt caacatctgc
    13561 cagctcattt tttctctgat tcatcaaatg gttaattcgt tcatggtcaa gagagaaact
    13621 gacgatagtt ctgcgccata atttctcggt atacaccaca ggcgatggat tttcagctac
    13681 caataatttc agtgcttttt ctatttgccc ataacggaga ttcgtccgtt gttcaatgcc
    13741 tcgtagggta agaccgtcat tctcgctaag tacgttgagt atttcatgaa tttgcgcctc
    13801 cgcaggaaag gcgctttcac ggaagaattt atgaatagcg cggtcttcac caccacaaag
    13861 caatatgcca actgcggaat ctatagcacg cccggcacgc cctacctgtt ggtagtaacc
    13921 gacaatagac cccggcattt gataatgaat gacgaaacct aaatcaggtt tatcaaaccc
    13981 catacctaat gccgtcgtcg cgaccagcac tttgatttta tttgccagca gcgcctgctc
    14041 cagatattcc ctggcagtat tagaatcttc cgcgccttca caagtcacgc cgctgtagta
    14101 agcaaatgca ctgataccat ttgtccttaa ccactcggcg acaagttcgg catcgcgagt
    14161 agtcagggta taaacaattc ccgatttgga aaactgaggg ataaccgttg ctaaccatgc
    14221 cagacgggat gactgttcac ctaagactaa ggcatcaagc gccaaacttt cgcgagccag
    14281 cgttccacgc tgaatcacaa tgtcacccaa ttgctgacgg atatcctcaa cgacacgatt
    14341 attcgctgtc gcggttgtac ccagaacagg ggtattcgca gtttcaagaa aatcctgatt
    14401 cgccaaccgt tcaggtgaaa tcagcagaca atctacacca ccccgcagca acttatcgct
    14461 aatgcgctgc cattcttccc tgtttgtaga attcaacgtt tcagccgtaa tacctaaccg
    14521 ttcagcggcg gcaacctggt tacgcatcag ggcaagcaaa ggagagatga taattgtcgg
    14581 gccagcgccg cgatcgcgga agattttact ggcgatgaag tacacggcac ttttccccca
    14641 gccagtgcgt tggacaacca gcagttttcg ccgctgatta accaccgcat ctatagcttc
    14701 ccattgccct tccctgaaag tagcgctggt gttacttaac atccgctgta acagcaattc
    14761 agctccgtgt ttctccatca tcttctccct cgtccgttaa cgcagtgatt agaacggata
    14821 atgttccggt ctttttgcga gagagatcaa aaagatgaag acaggagata agagaaatga
    14881 attactgcga gcaagctcgc agtaatttgt cgcagtaatt aactcaacag ctggaacgca
    14941 atcatcccaa caatggcacc aacagtgccg aggatggttt ccatcatggt ccaggttttc
    15001 agcgtttcgg cttcggtcgc gccggtaaat ttaccgaaca accagaaacc agcgtcgtta
    15061 acgtggctga caacaatcga accaccggcg atacaaatcg acagcgccgc catttgcgca
    15121 ccggaattgt tcagttgttc aatgaccggc atcaccagcc ccaccgccgt taaacaggca
    15181 acggtggcag acccctgaat gatgcgcact gcagctgcca gcacgaagca ggtgatagca
    15241 atcggcaggc ccatgccggt taacgcttcg cccagtgccg gacctacgcc agagtcaacc
    15301 agcacctgtt tgaacacgcc gcctgcacca atcaccagca gaatgatccc cgccggttgc
    15361 agcgcgtgac cgcaaatctc catcactttg tcttttggca tgccctgacg cattgccagg
    15421 ccataaatcg ctaccagaca agcaaccaga atcgcggtaa acggatgacc aataaactcg
    15481 aaccattcgt aagcggtaga gccttctggc acaaaacgcg cggcaatggt tttcagcccc
    15541 accagcacca gcggcaacag gatcagcgac aggctgaatc cgaaagatgg cattttacct
    15601 tcgccgagat gcggctcgct gatgtcgtca ggaatatgca gctcaacgta acggctgatg
    15661 aagttacccc acagcggccc ggcaataatc attcccggaa ttgccgcaca caggccaatc
    15721 aggatcatcc agccaaaatc ggcgttcatc tgcgatgcca gcagcattgg cgctggccct
    15781 ggcaccagga acgccgccgc tgccgccacg cctgcgaata atgggattat cagcttcacc
    15841 aggttcgtat cggtgtggcg cgccattgag aaagcaacgc taatcagcag aacaatcgcc
    15901 acttcaaaga acagcggtag cgcacagacc agccccgcaa ggccgatggc ataatgcgcg
    15961 cggctgtgac cgaaggattt gagcattttg acggcaatct gatcgactgc gccggtttca
    16021 tgtaagatct tgccaaacat agctcccagg gcgacaacca ccgccaggaa gccgagggtg
    16081 cctcccatcc ctttttccat cgtcgctgcg attttatcga gcggcatacc ggaaaaaagg
    16141 ccagccccca tggacaccac catcaaagcc aggaaagcgt gcatacgcgc cttcatgact
    16201 aaaaacagca gcagtaaaac agaccctact gctgttaaaa caagcgttaa tgtagtcact
    16261 acttatttgc cttttttaat aacctcaatg gtgcttgcca caacaccttc cagcggttga
    16321 tcgatatcca ccaccagtac atcggtttcg tccgcacccg gctcctgcag cgtttcaaac
    16381 tgcgtcacca acatttgggt tttaaagaaa tggcctttgc gcgctttcag gcggctttca
    16441 atcacgtcaa aatcgccttt caggtagatg aaagagagat tcggattacc ttcacgcagc
    16501 aagtcgcgat agtgtttttt caatgcagaa cagacgatca gtgacacttt attagtgcgc
    16561 tgcatagcaa acgcggcgtc gttcagcgcc tgcaaccagg gtttgcgatc gtcgtcattc
    16621 agcggttcgc cagacgccat tttttcgata ttgcgccgtg gatggaggaa atcgccatca
    16681 agaaacgcgg catgaagttg atgcgccact tcactggcga ccgcagattt gccgctgccc
    16741 gatacgccca tcaagacgta aatgtggtga tcatggttag tcgtgctcaa agtggtgccc
    16801 ctacaataca agaattaaaa atgttacggg taactgttat cggtaacatt gtccagccgg
    16861 acaatatcag aagcaatatc catccgtgcc ttaagtgtat aagtgtgagc tacttcaaat
    16921 ttgtaggctt aaatagatcc gcccggtgac aaggtgaaac ctaaatctaa cattttcggt
    16981 gtcacagatt cgccacgaat acgcgccagc aggcgttcag cgccaatact gcccatccgc
    17041 tcacgcggcg tcagcacgct cgctaaccgc ggctccatca cctggccaat gtcatgaccg
    17101 tggaaaccgg caatcgccat atcgtcagga accttcaatc ccagacgctg acattcaaac
    17161 gccgcgccga ccgccaggtc atcgttggta cagaacacgc catccagctg cggatattcc
    17221 cgccgcgcct ggcgaatcag ttcaataccg gaagagtaag aagaagattg ctcaaccatc
    17281 acgctatacg gcaccagacc tgcatccagc atcgcctgtt cgtatccctt ctgtttgatg
    17341 atagtacgtt cgtcgagacg cgcgccgaga taggcaatgt ggcaatgccc gcgagcaata
    17401 atggcggtgg tcatctggcg tgccgcttca aagttatcaa aaccgacggc gatatcgagg
    17461 cacggcgact ggctgtccat gagttccacc accgggatcc ccgccacttc aatcatcttt
    17521 aaggtgcgcg gcgtgtgggt acgttcggtg aggatcagtc cgtcgatatt ccaggagagc
    17581 atggattcga gacgttcttg ctccatttcc ggtttataac cgtagtgagc cagcatggtc
    17641 tgataaccgt gcgcgtcggt gacgctttcg attccgcgta atacttccgc gaaaacctgg
    17701 ttggtgagag aaggtaacag gacgccaatc gcccggctgg tggcgttaga gaggatatcg
    17761 ggcgcacgat tgggaatata gcccagttca tcaagagcgg cggcaatctt gccgcgtaga
    17821 gcgacggaaa cctgctccgg gttgcgtaaa aaacggctga ccgtcatttt ggtcacgcct
    17881 acacggtcag ccacatcctg aagtacgggt cttttctttt tcatcgtcct gaaggtacaa
    17941 aagagataga ttctcttagt ttaacacgga cgcatcagaa ccttcccggg gaaaacagtg
    18001 cttgtttaca attattagat atacactaaa tcaattgcat cgcattgtgc taacgacgcg
    18061 gtaaaacccc ggaagcgtgc atcagcacgc gaccggagcg agcgaggaag cctgcaaaaa
    18121 ggcaggcttc aaagatgccg ttgagtttta aaccggcggc agatcgaaca gtaacacttc
    18181 gctgtcgcta tccgcatgga tggagattgc ctgctcatcc cagattgcca gaccatcgct
    18241 ggtcgaggct ttcacgccgt taatggtgac attgcctttc accacctgga tccagacgcg
    18301 gcgttcagcg gcaatctgat gcaccgactg ctcatctttc agcaacgccc agcggtacag
    18361 ttccatatcc tgatgcactt tcaatgaacc atctcgcgca tccggcgaga gcaccagctg
    18421 tttgccctgt acggcatcga agcgacgctg ttcataacgc ggcgtaatac cgttttcttc
    18481 aggcatgatc cagatctgat acagatgcag acgctcggtg ctgcttgggt tgtactctga
    18541 gtgacgaata cccgtacccg cactcataat ctggaactca cccgccggaa cctgctcttt
    18601 attgcccatg ctgtcctgat gctcaacagt accttccagc acgtaggtca aaatttccat
    18661 atctttatgc gggtgagtgc cgaagccctg ccctgcttca atcacgtcgt cgttaatcac
    18721 gcgcagcgcg gagaagccca taaagttcgg atcgtaatag ttggcaaaag agaaagtatg
    18781 ccaggagtcc agccagccat gatttgcatg accacgttca tttgctttgc gtaagtagat
    18841 catttgtatt cgccccatga atgatttcga tgggcttagt gtggacccga tctgcctggg
    18901 atgatagagg gtgaaaattg acccctctgt tcaaaaaaat tgaactattc gaggggccat
    18961 caaacttact tcgcgagagt cactgtggag ggagaggctt gctcaaatcc gcgttcaagg
    19021 atttccagat tggtaagaac ttcagattcc ttgacgtaat ttggcgcacc gtgggtgatg
    19081 gtttgataca acgcatcata aacgcgcccg taatcgccca tctccggctt catctcttct
    19141 ctgaccgtca cgccctcgtc attgacatac tccagcacac cgaccgaatc atccgctgcg
    19201 aatcccggtt cgcccggcat aatattagcc ttcaggctgg tttcctgctg gtcgataccg
    19261 tatttaataa acgaaccttt cttaccgtga acgataaatt tcggataatc gattttcacc
    19321 agatggctgg ttttgacgat ggctttcagg tcgccataaa acagttgcgc ttcaaaggtg
    19381 tcgtcaggat tggctttatt acgcaggctg cagatgtcat aagcgacgtg atccgggcga
    19441 ccgaacagag aaataatctg gtccatcgta tgcacaccaa ggccatagaa cgcgccatcc
    19501 tgcggcaacc caggtttggt ttctgccacc gggcggtaat agtcaaaatg gctttccact
    19561 tcaacaatct ctcccagctt accgctttca atcgcttttt tcgccgtcag gaagcaggaa
    19621 tcaaagcgac gattctgata tggcgtgacg gtcagccctt tgcttttcgc cagtgcaaac
    19681 agctctttcg cctgcgcaag tgtcggagtg aacggttttt cgaccagcac atttttcccg
    19741 gcttccagcg cgcgcttcgc gtactcgaag tggctgtccg catgggtgca gacaacaacc
    19801 aacttaacat cgggatcgtt tagcacttcg tcgagatcgc tggtgaaatg gatatgagag
    19861 taaatgggag cctgttcttc cggcttcgca tggcgacgaa aaatatgcgc gacatgccag
    19921 ctatccttgc ggttaagtac atacggcaga tggtaacggg tggtgctttt gccgaagcca
    19981 ataaaggcgc agttgatgac catgatttcg tcctttttaa ggtggttatt tacaccttag
    20041 cgcaaagcgg acgtggttcc tactggagtg cgcataacga acacaagcac tcccgtggat
    20101 aaattgagaa cgaaagatca aaaaaaaagc cagcacccgg ctggctaagt aatactggaa
    20161 gcaatgtgag caatgtcgtg ctttcaggtt ctccgcgagg gtcttcctga tcgcgagaca
    20221 ataataatca ttctcattcg cacttgtcca acactttttg caaaaaaaat gcatttgact
    20281 cgcatttgaa agtcaatgat gttgaaaggg acatttaccc caaagaggac aaaggaatga
    20341 gtgagatagt aatacgccac gcagaaacac gggattacga ggccatcagg cagattcacg
    20401 cccagccgga ggtgtattgc aacacactac aggtgcctca tccttccgat catatgtggc
    20461 aggagcgact cgccgatcgt cccggcatca agcaactcgt cgcctgtatt gatggagacg
    20521 tcgtgggcca tctcaccatt gacgtgcaac agcacccacg ccgcagtcat gttgccgatt
    20581 ttggtatctg tgtcgactct cgttggaaga accgcggcgt cgccagcgcc ctgatgcgag
    20641 agatgattga aatgtgcgac aactggttgc gggtagatcg cattgaacta accgtgtttg
    20701 tcgataacgc cccggcaatt aaggtctata aaaaatacgg ctttgaaatt gaagggactg
    20761 gtaagaagta cgcat
//
LOCUS       DAKISA010000080        20757 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.080, whole genome shotgun sequence.
ACCESSION   DAKISA010000080 DAKISA010000000
VERSION     DAKISA010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20757
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.080"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..75
                     /locus_tag="OVW45_002939"
     CDS             <1..75
                     /locus_tag="OVW45_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCV5527338.1"
                     /translation="ARFSGMVKTALTLAVTTTLKELTP"
     gene            72..785
                     /locus_tag="OVW45_002940"
     CDS             72..785
                     /locus_tag="OVW45_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCV5527339.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLVQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(757..1434)
                     /locus_tag="OVW45_002941"
     CDS             complement(757..1434)
                     /locus_tag="OVW45_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCV5527340.1"
                     /translation="MLTLNQISYRWPSAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(1428..2105)
                     /locus_tag="OVW45_002942"
     CDS             complement(1428..2105)
                     /locus_tag="OVW45_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCV5527341.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSLYLQLSGCTFSVE
                     EEVAFGPENLCLAETEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWALERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(2093..2800)
                     /locus_tag="OVW45_002943"
     CDS             complement(2093..2800)
                     /locus_tag="OVW45_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCV5527342.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVAWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIILLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(2801..3379)
                     /locus_tag="OVW45_002944"
     CDS             complement(2801..3379)
                     /locus_tag="OVW45_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCV5527343.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(3402..3833)
                     /locus_tag="OVW45_002945"
     CDS             complement(3402..3833)
                     /locus_tag="OVW45_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCV5527344.1"
                     /translation="MMTLPYPYSSPARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            4205..5224
                     /gene="epd"
                     /locus_tag="OVW45_002946"
     CDS             4205..5224
                     /gene="epd"
                     /locus_tag="OVW45_002946"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCV5527345.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            5274..6437
                     /gene="pgk"
                     /locus_tag="OVW45_002947"
     CDS             5274..6437
                     /gene="pgk"
                     /locus_tag="OVW45_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCV5527346.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETASATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            6652..7731
                     /gene="fbaA"
                     /locus_tag="OVW45_002948"
     CDS             6652..7731
                     /gene="fbaA"
                     /locus_tag="OVW45_002948"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCV5527347.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            8089..8949
                     /gene="mscS"
                     /locus_tag="OVW45_002949"
     CDS             8089..8949
                     /gene="mscS"
                     /locus_tag="OVW45_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCV5527348.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            9088..9723
                     /gene="argO"
                     /locus_tag="OVW45_002950"
     CDS             9088..9723
                     /gene="argO"
                     /locus_tag="OVW45_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCV5527349.1"
                     /translation="MFSYYFQGIALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            9816..10556
                     /locus_tag="OVW45_002951"
     CDS             9816..10556
                     /locus_tag="OVW45_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCV5527350.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            10723..11619
                     /locus_tag="OVW45_002952"
     CDS             10723..11619
                     /locus_tag="OVW45_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCV5527351.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKNIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(11616..13094)
                     /gene="scpC"
                     /locus_tag="OVW45_002953"
     CDS             complement(11616..13094)
                     /gene="scpC"
                     /locus_tag="OVW45_002953"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HCV5527352.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(13118..13903)
                     /gene="scpB"
                     /locus_tag="OVW45_002954"
     CDS             complement(13118..13903)
                     /gene="scpB"
                     /locus_tag="OVW45_002954"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCV5527353.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMMPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(13914..14909)
                     /gene="meaB"
                     /locus_tag="OVW45_002955"
     CDS             complement(13914..14909)
                     /gene="meaB"
                     /locus_tag="OVW45_002955"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCV5527354.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(14902..17046)
                     /gene="scpA"
                     /locus_tag="OVW45_002956"
     CDS             complement(14902..17046)
                     /gene="scpA"
                     /locus_tag="OVW45_002956"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCV5527355.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKQLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAADHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(17250..18143)
                     /gene="argP"
                     /locus_tag="OVW45_002957"
     CDS             complement(17250..18143)
                     /gene="argP"
                     /locus_tag="OVW45_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCV5527356.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            18573..19232
                     /gene="rpiA"
                     /locus_tag="OVW45_002958"
     CDS             18573..19232
                     /gene="rpiA"
                     /locus_tag="OVW45_002958"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCV5527357.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            19488..20720
                     /gene="serA"
                     /locus_tag="OVW45_002959"
     CDS             19488..20720
                     /gene="serA"
                     /locus_tag="OVW45_002959"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCV5527358.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
BASE COUNT         5006 a         5231 c         5504 g         5016 t
ORIGIN      
        1 gcgcgtttta gcggaatggt caaaacggcg ctgacgctgg cagtaacgac caccttaaag
       61 gaattaacgc cgtgaaaatt gaattaacgg tgaatgggct gaacgtgcaa gcgcagtacc
      121 atgatgaaga gatagaacgc gtgcataaac cgttgctgcg tatgctggcg gccttacaga
      181 cggtaaatcc gcagcggcgt acggtggttt ttctttgcgc tccgccgggg acgggtaaat
      241 ctaccctaac caccttctgg gaatatctcg tgcaacaaga cccggagctg cccgctattc
      301 agacgctccc gatggatggt tttcaccatt acaatagctg gctggacgcg catcaattgc
      361 gccccttcaa aggcgcacca gagacatttg acgttgcgaa actggcggaa aatctgcgcc
      421 aggtggtgga aggggattgt acgtggccgc agtacgatcg acaaaagcat gatcctgttg
      481 aagatgcgtt gcacgttacc gcaccactcg tcatcgtcga aggaaactgg ttgttgctgg
      541 acgacgagaa gtggtgccag cttgcgcaat tttgtgattt ctcgatcttt atcaacgcgc
      601 cagctactgc gttgcgggag cgtctggtgg ggcgcaagct ggcgggaggg gtatcgctgg
      661 cagatgcaga agccttttat gaccgtaccg acgggccgaa tgttcgccgg gtgctggaag
      721 agagtctgcc tgcaaatttg accttaatga tgaccgctac gggtgaatat cgtctggtgg
      781 attaacgttc ctgattacac catcttccag tcgcaccaca cgagagaaat gacgccgggt
      841 aaatgcggcg tcatgactga ttgctaccac gctcgtgcct cgctggcggc acttctccag
      901 ccagctttca aacacgctca accaattttc gtcaaaatcc cgactcggct cgtccagcag
      961 taatagtggc ggagagagtg cttccaggca ggcgacggcg accatccgtc gctgcgcact
     1021 atgcagatcc aacgggtgcg agttggcagt gtctgctaac tgacaacatt gcagggcagc
     1081 atgggtacgt tgagtgattt catccgccgg gcatttttgc agtttcaaac caaatgcgat
     1141 ctcgtcagcg acggtgctgt gaaaaagttg gttttccgct tcctgaaaca gtacgccaac
     1201 ctttgccgcg cgctggcggt ttttcaggtt tttcatcgcc tgttgttgca gcataaccgt
     1261 accggctgtg ggcgtaagaa gacctgccat tactcgtaac agcgtcgatt ttcccgcgcc
     1321 attgtcgccg gtcagcgcca accattcacc ttgtttgagt tgcagagaaa tgtcgcaaag
     1381 acaatccgtt gctgcgctag gccagcgata agatatctga tttaacgtta acatagtggc
     1441 tggagcgctc cgttttgcaa ttgccacgct tgctggcagt agttcaggaa aggtgtgcga
     1501 tgacgttcaa aaagaataat cagcgaacca cgttcaagcg cccagtgttg taagcgctgc
     1561 aacagcattt cgctggcctg cggcgtcagg cgactaaagg cttcatcaag aattaagagc
     1621 tttggctgca tcgcgatggc gcaggcgatg accacgcgtt gtgtctctcc accggagagt
     1681 gttgccgggt gacgatggcg caacggctgg cactcggtca gagtcagggc ggcatcaatc
     1741 cgcgccataa tctctgtttc agcaagacac agattttctg gcccaaaggc aacttcttct
     1801 tccacgctaa aggtacagcc ggagagctgc aaatagagcg attgctggac gagctggatg
     1861 gtggcggatt gttcattaag cgggagatgc ccaatgggcg taccgaggag cgtccccgtg
     1921 cctgttatct ctcctggcag aaagtccgga taccagcccg ccatcagctg tgccagcgta
     1981 cttttgccac tgccgttgtc accaaagatg gcgaccatcc ccggtttgac atagtgaaag
     2041 tcataacaga acggaggatg cgttgagtgt gtcgggcaat aacggaactg ttctaacgta
     2101 accatatcca cgctccgaat tctgttagca tcagcaagat catggtgtaa cgcgctacgc
     2161 gttgtagggt gctatcggct ggtgcccaca acgttgttcg tcggttgtgg atccgaaatg
     2221 cccgcatatc cagcgccgca ccacggacag ccagatcgtt aagcgcattg tgtgttagca
     2281 gaataattaa cgcgggcatc gcacgtaaac gctgatacca gccctcatcc agcggcacgc
     2341 cgcgggcacg ctgcgcttca tgaataatcg ctaattgccg ttttaactgc tcaacgacca
     2401 ataacggtcc ggcaaacaga taagcgacgc ctggcggcag gcgagaagca aacagagcgc
     2461 ggataaaaca ttgcacgggg acgtactgca tccacaactg tgaggttgaa acaatcgcca
     2521 gaatccgcaa ccagagcgta atagcatgcg tccaacgctc cggcgaacgc ggcgtgccgc
     2581 ttagccattc tgttagccag ccaccgtgta ccagccacag cccggctccc agcgagaaca
     2641 tcagccaggc gacatatttt gcccgccgcc gcgtggcctt tagcgcaata agacaaaaaa
     2701 aggtcgccgc gctatagatc ggcaaaatgg tctgcgcggg caggataagc gtggtgcagg
     2761 ccgccaatgc ccacagtgtt aatgaggtaa atggatgcat ttagcgcacg gcagccattg
     2821 ccggaaaatg gcgcgtggtg cgaatcggca gctggcgcag taaaagccag acaatcaccg
     2881 ctgtaagaat tttatcgacc aggttcgcgc ctatcaccgt aatcgccacc gactccacca
     2941 ggttttgccc catcgagtgc atccaggcaa cgaacaaatc ggctccgcta ccggtgacac
     3001 cgccgaacag cgcagtacgc agcggtacag cgacgaccgt taccgcaagc gtaataatca
     3061 cgccgctgac aacgactttt ggcagggtgc gaaaccaacc cgctcgcgcc agccaaccgg
     3121 cgaccaggcc gatgaccatc gcgacgggtg caaatgcggc ggcgatcggg tcggtcagca
     3181 atccccagag taaattggtc agtaagccgg tcagcattcc gatcaccgga ccaagcagta
     3241 ccgcgctaat aagtgtgccg atggagtcga gaaatatagg taatttgacc atgctggcca
     3301 gttgcccgcc gatcatattg atggcgatag atataacgat caacaccagt gcctgactgg
     3361 aaaagtggct acgcgccata agaaaacctc tttgaaagta gttatttaac gggggaaata
     3421 tgcaactcgt cgtaaccaga aagcgcgcaa ggttggcggc taaacgccag ttcctcaagg
     3481 ctaccgatca ccagttcaag cgtggtccgc acggtacagc cgggcatcat atgaccgcca
     3541 agcatcgtgc cgtgtggatc ggaaacgcag agatgaagat gctcaccact ctgttccagc
     3601 gtgccgttta gtgagatcac ttcgaatgta ccgttaagga gcgtggtgcc ttcttgcccg
     3661 gcatagcgca aggcgacgtc agtcaggcta ccggtacagc ctgcaatcca ggcggcatga
     3721 agttgatgct gctgtgcaaa agcgtgtagc tgggaaaaaa cttcctgccc ggggaggagg
     3781 cgcagtgcat aaaagcgtgc gggggaagaa tatgggtagg gcaatgtcat cattggagta
     3841 ctccgaaact cggacgtatt gcggataata tcgacgctgc atcagtcata ttctgatata
     3901 agtcagggtt tgtgacggca caacgaaaag attttgctac gcttattcgt gattctgcaa
     3961 agtggaataa attccacagg ggcggggctg aaaacgtgac gtaagtcaga taattgtttt
     4021 ctttgactgg acaatcattc cttttattcc acgtttcgct tatcctagct gaagcgtttc
     4081 agtcgattaa atgttcgaca attaaccaat cagtcgcagt ttgcgacagg taaggtttcc
     4141 ccggacgatt tgctggatta ctctgtctgg caaatttgct cagggaaaac cttgcaggag
     4201 atctatgacc gtacgcgtag cgataaatgg cttcggtcgc atcgggcgta atgtggttcg
     4261 tgctttgtat gaatccggac gccgggcgga aattaccgtg gtggcaatca acgaactggc
     4321 ggatgctgcg ggcatggcgc atttgttgaa atatgacacc agccatggcc gttttgcatg
     4381 ggaagtacga caggaacgcg atcaactttt tgttggtgat gacgccatcc gcgtattgca
     4441 tgaacgttca ctgcaatcgc tcccctggcg tgaacttggc gttgatgtag tcctcgactg
     4501 caccggcgta tatggctccc gcgagcatgg cgaagcgcat attgccgccg gggccaaaaa
     4561 agtgctcttt tcacatcctg gcagtaacga tctcgacgcg accgttgttt acggcgtcaa
     4621 tcaggatcaa cttcgtgcgg aacaccgcat cgtttctaac gcttcctgta ccacgaattg
     4681 cataattccc gtcatcaaat tgttagatga tgcgtacggt attgagtccg gcactgtgac
     4741 cacaattcac tccgccatgc acgatcaaca ggttattgat gcataccatc ctgacctgcg
     4801 tcgcacccgg gcagccagcc agtcgatcat tccggtcgat actaaactgg ccgccggtat
     4861 cacacgattt tttccgcaat ttaacgatcg ctttgaagcg attgcggtac gtgtgccaac
     4921 cataaatgtg acggcaatcg atttaagcgt gacggtgaag aaacctgtaa aagccaatga
     4981 agtcaacctg ttgctgcaaa aagcagcaca aggtgcattt catggtatag ttgactatac
     5041 ggaattgccg ttggtctctg tagattttaa ccacgatccg cacagtgcca ttgtcgatgg
     5101 cacccaaacc cgggtcagtg gcgcacacct gatcaaaacg ttggtctggt gcgataacga
     5161 atggggcttt gctaaccgaa tgctcgacac gacgttagct atggctactg ttgctttcag
     5221 gtaagacgca agcagcgtct gcaaaacttt tagaatcaac gagaggattc accatgtctg
     5281 taattaagat gaccgatctg gatcttgctg ggaaacgtgt atttatccgt gcggatctga
     5341 acgtaccagt aaaagacggg aaagtaacca gcgacgcgcg tatccgtgct tctctgccga
     5401 ccatcgaact ggccctgaaa caaggcgcga aagtgatggt aacttcccac ctgggtcgtc
     5461 ctaccgaagg cgagtacaac gaagaattct ctctgctgcc ggttgttaac tacctgaaag
     5521 acaaactgtc taacccggtt cgtctggtta aagattacct cgacggcgtt gacgttgctg
     5581 aaggtgaact ggttgttctg gaaaacgttc gcttcaacaa aggcgagaag aaagacgacg
     5641 aaaccctgtc caaaaaatac gctgcactgt gtgacgtgtt cgtaatggac gcattcggta
     5701 ctgctcaccg cgcgcaggct tctactcacg gtatcggtaa attcgctgat gttgcgtgcg
     5761 caggcccgct gttggcagct gaactggacg cgctgggtaa agcactgaaa gaacctgctc
     5821 gcccgatggt ggctatcgtt ggtggttcta aagtatctac caaactgacc gttctggact
     5881 ccctgtctaa aatcgctgac cagctgattg ttggtggtgg tatcgctaac acctttatcg
     5941 cggcacaagg ccacgatgtg ggtaaatccc tgtacgaagc tgacctggtt gacgaagcta
     6001 aacgtctgct gaccacctgc aacatcccgg ttccgtctga tgttcgcgta gcaaccgagt
     6061 tctctgaaac tgcatcggct accctgaaat ctgttaacga tgtgaaagct gacgagcaga
     6121 tcctggatat cggtgatgct tccgctcagg aactggctga aatcctgaag aatgcgaaaa
     6181 ccattctgtg gaacggtccg gttggcgtgt tcgaattccc gaacttccgc aaaggtactg
     6241 aaatcgtggc taacgctatc gcagacagcg aagcgttctc catcgctggc ggcggcgaca
     6301 ctctggcagc aatcgacctg ttcggcattg ctgacaaaat ctcctacatc tccactggcg
     6361 gcggcgcatt cctcgaattc gtggaaggta aagtactgcc tgcagtagcg atgctcgaag
     6421 agcgcgctaa gaagtaaaaa atcacagggc agggaaacct gcccttgttt cagcgcgctt
     6481 ttagagcacg cattttttca tactctaaat aattcgagtt gcaggaaggc gacaagagag
     6541 tgaatcccca ggagcttaca taagtaagtg actggggtga gcgaacgcag acgcagcaca
     6601 tgcaacttga agtatgatga gtataaggcc cgacgataca ggacaagaga catgtctaag
     6661 atttttgatt tcgtaaaacc tggcgtaatc actggtgatg acgtacagaa agttttccag
     6721 gtagcaaaag aaaacaactt cgcactgcca gcagtaaact gcgtcggtac tgactccatc
     6781 aacgccgtac tggaaaccgc tgctaaagtt aaagcgccgg ttatcgttca gttctccaac
     6841 ggtggtgctt cctttatcgc tggtaaaggc gtgaaatctg acgttccgca gggtgctgct
     6901 atcctgggcg cgatctctgg tgcgcatcac gttcaccaga tggctgaaca ttatggtgtt
     6961 ccggttatcc tgcacactga ccactgcgcg aagaaactgc tgccgtggat cgacggtctg
     7021 ctggacgcgg gtgaaaaaca cttcgcagct accggtaagc cgctgttctc ttctcacatg
     7081 atcgacctgt ctgaagaatc tctgcaagag aacatcgaaa tctgctctaa atacctggag
     7141 cgcatgtcca aaatcggcat gactctggaa atcgaactgg gttgcaccgg tggtgaagaa
     7201 gacggcgtgg acaacagcca catggacgct tctgcactgt acacccagcc ggaagacgtt
     7261 gattacgcat acaccgaact gagcaaaatc agcccgcgtt tcaccatcgc tgcgtccttc
     7321 ggtaacgtac acggtgttta caagccgggt aacgtggttc tgactccgac catcctgcgt
     7381 gattctcagg aatatgtttc caagaaacac aacctgccgc acaacagcct gaacttcgta
     7441 ttccacggtg gttccggttc tactgctcag gaaatcaaag actccgtaag ctacggcgta
     7501 gtaaaaatga acatcgatac cgatacccaa tgggcaacct gggaaggcgt tctgaactac
     7561 tacaaagcga acgaagctta tctgcagggg cagttgggta acccgaaagg cgaagatcag
     7621 ccgaacaaga aatactacga tccgcgcgta tggctgcgtg ccggtcagac ttcgatgatc
     7681 gctcgtctgg aaaaagcatt ccaggaactg aacgcgatcg acgttctgta agatattcct
     7741 ttctgcttat ctcaaggccc gctctgcggg tctttttttc gccaaaagca aaattgcctg
     7801 atgcgctacg cttatcaggc ctacacgtat cctgcaatat attgaatttg caggtttttg
     7861 taggccggat aaggcgttca cgccgcatcc ggcatgacaa agcgcacatt gatatctgtc
     7921 tgagccccgc tctgcgggga ttttttcgcc aaaagcaagg tgattcagat gagaaatctg
     7981 tgatctattt ggcaaaatta tgctttattg tttacccttg tcagactgcc cgtcataagg
     8041 cggcggagtg tatttctcca ttttgagtca gttgaaaagg aatattgaat ggaagatttg
     8101 aatgttgtcg atagcataaa cggcgcggga agctggctgg tagctaacca ggcgctgctg
     8161 ctaagttatg cagtaaacat cgtggcggca ctcgcgatca tcatcgttgg tttgattatc
     8221 gcgcggatga tttccaacgc ggtgaatcgc ctgatgatct cccgtaaaat cgatgccact
     8281 gttgctgatt ttctttctgc attagtccgt tacggtatta tcgcctttac gctaatcgct
     8341 gccctgggac gcgtgggtgt acaaacagcg tcagtcattg ctgtactcgg tgccgcaggc
     8401 ttagctgttg gtctggcttt gcaggggtca ctttctaacc tggccgctgg cgtgttactt
     8461 gtcatgttcc gcccgttccg tgccggagaa tatgttgatc tcggcggcgt agccggtact
     8521 gtgctgagtg tgcagatttt ctccaccacc atgcgtactg cagacggtaa aattatcgtt
     8581 attccgaacg gtaaaattat tgccggaaat attattaact tctctcgcga gccagttcgc
     8641 cgtaacgaat ttattattgg cgtggcgtat gattccgata tcgatcaggt taagcagatc
     8701 ctgaccaata ttatccagtc tgaagatcgc attttgaaag atcgcgaaat gactgtgcgt
     8761 ctgaacgaac ttggtgcatc gtcgattaat ttcgtggtcc gcgtctggag caacagcggc
     8821 gatctgcaaa acgtgtactg ggatgtgctg gagcgtatta aacgtgaatt tgatgccgcc
     8881 ggtatcagct tcccgtaccc gcaaatggat gtgaacttta agcgggtgaa agaagacaaa
     8941 gctgcgtaat caacgctgag gccagataat actctctggc ctctctctta ttagttattc
     9001 tgattgccaa ttaatattat caatttccgc taataacaat cccgcgatat agtctctgca
     9061 tcagatactt aattcggaat atccaacgtg ttttcttatt actttcaagg tattgctctt
     9121 ggggcagcta tgattctgcc gctcggtcca caaaatgctt ttgtgatgaa tcagggcatt
     9181 cgtcgtcagt accacattat gattgcctta ctttgtgcta tcagcgattt ggtcctgatt
     9241 tgcgccggga tttttggtgg cagcgcgtta ttgatgcagt cgccgtggtt gctggcgctg
     9301 gtcacctggg gcggcgtagc cttcttgctg tggtatggtt ttggcgcttt taaaacagcg
     9361 atgagtagta atattgagtt agctagtgcc gaagtcctga agcaaggcag atggaaaatt
     9421 atcgccacca tgctggcagt gacctggctg aatccgcatg tttacctgga tacttttgtt
     9481 gtactgggca gccttggcgg gcagcttgac gtcgaaccaa aacgttggtt tgcgctcggg
     9541 acaattagcg cctctttcct gtggttcttt ggtctggcta ttctcgcagc ctggctggca
     9601 ccgcgcctgc gcacggcaaa atcacagcgc attatcaatc tggtagtggg atgtgtcatg
     9661 tggtttatag ccttgcagct ggcgagagac ggtattgctc atgcacaagc cttgttcagt
     9721 taggcgctat ctgatggaaa aataaaacag aggcgctaag cttgcctcca gaggtcctga
     9781 attttgggca agtcaattaa cacggagaga ctaacgtgaa gttcaaagtt atcgccctgg
     9841 cggcattaat gggtattagc gggatggcag cgcaggctaa cgaattgccg gatggaccgc
     9901 atattgtcac ctccggtacg gcaagtgtgg atgcggtgcc agacattgcc actcttgcga
     9961 ttgaagttaa cgtggccgcg aaggatgccg ctacagccaa gaaacaggca gatgagcgcg
    10021 tcgcacaata catttccttc cttgaactca atcagatcgc gaaaaaagat atcagctcag
    10081 cgaacttacg cacccagcca gattatgatt atcaggatgg taaaagtatc ctgaaaggct
    10141 accgcgctgt gagaacggtg gaagtcacgc tccgtcagtt agacaaactg aattccttgc
    10201 tggatggcgc gctgaaggcg ggtcttaacg aaattcgttc tgtgtcgctg ggcgtggcgc
    10261 agccggatgc ctataaagac aaagcgcgta aggcagcgat tgataacgcg attcatcagg
    10321 cgcaggaact ggcgaacggc tttcatcgta aactggggcc ggtatatagc gtgcgctacc
    10381 atgtttccaa ctatcagccc agcccaatgg tgcggatgat gaaagccgat gccgcgccgg
    10441 tgtctgccca ggaaacttac gagcaggccg ctattcagtt tgatgatcag gtcgatgtgg
    10501 tcttccagtt agaacctgtg gatcaacaac ccgctaaaac acctgcagca caataatact
    10561 tgtagcccga cgtattcgtc gggctaatgc ctttacccga tattgcctcc tttttctgta
    10621 attttctgta aaaccatgac gtaatcacct atctttgctc gcggtttcat caaggatagg
    10681 ctgactgaaa cggttaagcg gacaaggatg cggggagggg ggatggacat ttttatttcg
    10741 aaaaagatgc gcaatttcat tttattagcg caaaccaata atattgcccg ggcggcagaa
    10801 aaaatccata tgacggcttc gccatttggt aaaaacattg ccgcgctgga agaacaaatt
    10861 ggctatacgc tatttacccg caaagataat aatatcagcc tcaataaagc cggtcaggaa
    10921 ctgtatcaaa aactgttccc ggtttatcaa cgactttctg cgattgataa tgaaatccat
    10981 aactcagggc gtcgttcacg agaaattgtg attggcatag ataatactta tccgacaatt
    11041 attttcgatc agttgattag tctcggagat aagtatgaag gcgtgaccgc ccagccagtt
    11101 gagttcagcg aaaacggcgt tatcgataat ctttttgacc gtcagttaga ttttattatc
    11161 tccccacagc atgtatctgc acgagttcag gaactggaaa acctgactat cagtgaatta
    11221 ccaccgctgc gattgggctt tctcgtttct cgccgttatg aagagcgtca agagcaagag
    11281 ctgcttcagg agctgccctg gctacaaatg cgctttcaaa accgggctaa cttcgaagca
    11341 atgattgatg cgaatatgcg accttgtggg atcaatccca ccattattta tcgtccctac
    11401 agttttatgg ccaaaatcag cgccgtggag cgcggacatt ttctgacggt gatcccgcat
    11461 tttgcctggc gactggtgaa tccggcaacg ctgaaatatt ttgatgcgcc gcacaggccg
    11521 atgtatatgc aggaatatct ttattcaatc agaaatcatc gttataccgc cacgatgctt
    11581 cagcatattg ctgaagatcg tgacgggaca aaccattaac ccagcatcga gccggttgca
    11641 attaaattac ggtgtaagtc gaagacgtgg ctaagatcgt ggtgaatatg tccgccgggc
    11701 gcattttcca gatagcgatg cagataatcc cgatacatag gatgtgcaca attatcaatg
    11761 atagtgcggg cgcgttgaag cggggaaaga ccgcgcagat cggcgatccc ttgttcggtg
    11821 atgatcactt tgacgctgtg ttcgctgtga tcaacatggc tgcacattgg cacgacggtt
    11881 gagatcttgc cttctttagc aatcgacgga gccataaaga tcgacagata cgcgttgcgt
    11941 tcaaaatcac cgctgccgcc gatgccgttc atcagatcga ccccggctac gtgcgttgaa
    12001 tttgcatgcc cgtaaatatc aaactccagg ccgacgttca gagcgatgac gcccagacga
    12061 cggatgattt ccgggttatt ggaaatctcc tgcggacgca acacaatgcg gctggcaaag
    12121 taatccatat tgtcgtaaat cttgcgcagg gaatcggccg agattgtcag gctggaggcg
    12181 ctggccccgc tgattttgcc ggtttccagt aaatgcacca ccgattcctg tagcacttcc
    12241 gaatacatca tgaacggagg aatttccggg ttttccccca gacgcgccat taccgcatta
    12301 ttgatattgc ccacgccact ttgcagcggc agaaattccg gcggaatacg cccatgcgcc
    12361 atttcctgca ataagaacgt gaccacgtta tcggcaatct gctggcacat gggattttgc
    12421 ttatccagca tattaccggc gtcgggcaag ttggtttcca cgacggcgac aatctttttc
    12481 ggatcgattt gcacatagcg ggtaccgacg cgatccattg catggaagat cgacacgcta
    12541 ttgcgccgcg gtggtgcgcc aggaatcaca atatccgcca gttctgcaac gcgcggatcg
    12601 tgatagtgat taagttcaat gatcactttc ttcgcccgca gcagccatgt cggcgcatta
    12661 ccgatcccgc tggttaacca gactcgacca tccggtgcca gtgccgatgc ttcgatgacg
    12721 gcaacatcaa tgtcgccgaa gaaaccgtaa ttgaccattt gcgccacttc gctcaaatgc
    12781 aggtcaacga aactcaccgc gccctgattg atctttttac gtaaaccgga cgatgtttga
    12841 tatggcgcac gccaggaaac agcatcggcg tcagaaagta catcgtcagc ggcggcgctg
    12901 attgacgccc ccgtcagcag gcgaatttga tacggctttt tggcctcatg ctgttcgtta
    12961 gctctgcggg caatcgcggt cggtaaggct ttcggcgaac ccgctggggt aaagccgcta
    13021 aatgccacca tgtcgttatg ctggataatt tctgccgctt catcggcggt catccttgtc
    13081 cactgagttt ccatttttac tccttcgttc gcagggatta atgaccaacg aaattaggtt
    13141 tacgtttttc gaggaaagcg ttcatccctt cctggtaatc ttcgctgtca taaaccgcgc
    13201 ggcgcatccc ctgaatacgt tcaaattcat cggagttcat ggtgtgcgct tcgcccagta
    13261 cacgcagctc ttctttgata acagcaatgg ctaacggcgc tttctcagag atgtggtgcg
    13321 ccatttgtaa ggtgaaatct tccagttctt ccacttccac aacatggttg aggatgccga
    13381 cagccaacgc gcgctgggcg gtgattggcg aagcggtaaa aatcagctct ttgacaatgt
    13441 ggaagcccgc gtcgcgggtc aggttgtgaa tgccgaccag gttatacggg acgccgaggt
    13501 ttacaggcat cattgagaag gttgaggtac tggcggcgat gatcagatcg gaactcatga
    13561 tcatttcaaa tgcgccaccc caaacactac cttccaccat cgaaatgatc ggtttcggga
    13621 atttttggat catgcgggtg atttgacgca atggatcatc ataggagagc ggatcgcgac
    13681 cgccagacgg cagttcgtga atatcgtgac ctgcggagaa gactttggat ccacttggcg
    13741 cccgcaaaat gatacagcga atttccggcc ggttgagatc gcttaacgcc tgcataagat
    13801 catcaataaa gactttactt aaggcattaa gttttcgtcc atagttaaac tcaatgaccg
    13861 ccactttgtt gatagtggca acgttcacat actgataaga cataaaaatt cctttaatca
    13921 aaatattgcg tctggataaa ttcactgagc tgccgcaggc cggtgcgcgg tgagagcgta
    13981 ttgtttttga ccgctaaaag cgtctggcgg taatagcgat cgaaatcttc attcgcgaac
    14041 aggtgattca gtacttcttc ttcggtctgc ttacgcagcc attccaccga ttgttgttgc
    14101 cgcacttgtt gtaaacgacc actggcagtt agcgcggttt tgaagtcgat gatggcgtgc
    14161 cagatctcat cgattccacg tttttccagt gcgctacaag tcagaacccg tggctgccat
    14221 tcgtcgtatt tacgtcgcag aatatgcagg gcactctcgt acatatgccg ggcaatggcg
    14281 acattggtat ggttatcgcc atcgtctttg ttgataacga gcagatcagc cacttccatc
    14341 agcccttttt taatgccctg cagatcatcg ccgccaccgg caatttgcaa cgagataaaa
    14401 cagtccacca tgcgggcgac ttccgtttcc gactgcccga cgccaaccgt ttcgacaatc
    14461 actacgtcat aacccgccgc ttcgcataac agcattaatt cccgtgctcg ctgactggca
    14521 ccgcccagat gaccggagga gggtaccggg cgaataaacg ccgcctcggc acgcgccagg
    14581 tcattcatgc gggttttatc cccgagaatg ctaccgccag tgaccgggct gctgggatcg
    14641 accgcaataa cagcaacctt taatccctca cgaatcaaca acatgccaaa ggcttcaaga
    14701 aaggtacttt tccccgcgcc gggtgtgccg gtaacgccca gtcgcagggc gttaccacag
    14761 aacggcataa tggcgtcaag cagctgtgta cttagcgcct gatgacgtgg gtgacggctt
    14821 tccaccagcg tcatggcctg ggcgagcgtg gcacgctcac cctgacgtaa gcggcgaata
    14881 ctttctgcca gcgtggcttc attaatcatg atgctggctt atcagattca gtacgtcgcg
    14941 cacactgtcg agcataggtg tacctggacc ataaatcgcc gccacgccgc gctcttgcag
    15001 gaaggcgtaa tcctgcggcg gaatgacgcc acccgcgacc acgcagatat cttcgcgtcc
    15061 ccattttttc agcgcttcga ccagttccgg gatcagcgtt ttatgatcgg cagccagtga
    15121 ggatgcgccc actacgtgaa cgtcgttttc tacggccagg cgggcgatct cttcaggtgt
    15181 agagaacatc gggcttaaat ctacgtcgaa accgagatcg gaataggcgc tggcgatcac
    15241 tttcgcgccg cgatcgtgtc catcctggcc catcttagcg atcagaatgc gcgggcgacg
    15301 accattgtcg gcaaggaact gctccgtttg cgcaacaatg gcatcgaact cggaggccga
    15361 tttctcagac tgatgatagc tttgcgcaat cacgccggta acacactggc ttggcaccag
    15421 ataacggtcg aaagcgactt ccagcgcatc ggaaatttca cccagggtgg cgcgaacgcg
    15481 agcggcatta acagcggcag ccagcaggtt ttcgttatgc tgtgcggcgt gagtcagggc
    15541 gttcaacgcg gcggttacgg cggcatcatc acgggtggcg cgaatgcgtt ccagcgaagc
    15601 aatttgctcg ttacgcacca tcacgttgtc gatctcaagt acatcggttt cgtcttcgtg
    15661 atccagtttg tacttgttga caccaacgat gacacgcttg ccttggtcga tcagcgactg
    15721 ttcgcgcgct gaggcctctt cgatcattcg ttttggcaga cctgcttcga tcgctttcgc
    15781 catgccaccg gcttcgtcga tctgttggat aatagctctg gcttgtttga cgatttgatc
    15841 ggtcagcgac tcaatgtaat aggatccggc cagtggatcg acggtgcggc agagttctga
    15901 ttcttcctgg atgatgatct gggtgttgcg ggcaatgcgt gctgagaaat cggtaggcaa
    15961 accaagcgct tcgtcaaagg cgttggtatg cagtgactga gtaccgccca gcgtcgcagc
    16021 cagtgcttca atggtggtgc ggataacgtt gttatacgga tcctgttcag tcaggctcca
    16081 gcctgaggtc tggcagtggg tacgcagcgc cagtgatttc ggatcctgtg cgccaaatcc
    16141 actgaccgct tcgctccata aataacgtgc cgcacgcaac atggcgacgt tcataaacag
    16201 atccatgccg ataccgaaga agaacgacag gcgaggagcg aagtcatcaa ttttcagtcc
    16261 ggcagagatt gctgctttga tgtactcaat cccatcggcg agcgtaaatg ctacctgctg
    16321 cacgcagttg gcacccgctt cccccatgtg gtaaccgctg atactgatgg tattaaatcg
    16381 cggcatgttg ccggaacacc aggcgatgat gtcggcgata atgcgcattg acggttttgg
    16441 tgggtaaata taggtgttgc ggcagaggta ctctttgaga atatcgtttt gaatggtgcc
    16501 ggtcagttta tcaggtgtaa caccttgctc ttctgcggcg acgatataaa acgccagtac
    16561 tggtagcact gcgccattca tggtcatcga aaccgacatt ttatccagcg ggatctggtc
    16621 gaacaggact ttcatatctt ccacggtgtc gatagcgacg cccgctttgc cgacgtcacc
    16681 cgccacgcgt gggttatcgg agtcgtagcc acggtgggtg gcaaggtcaa acgcaacgga
    16741 aagacctttt tgcccggcgg ccaggttacg gcgataaaaa gcgttggact cttttgctgt
    16801 tgaaaaacca gcatactgac ggatggtcca cggttgggcg gtatacatag tggcacgcgg
    16861 gccacgaacg tagggcggca aaccaggaag ggtacctgtc acctccagat tatcgagatc
    16921 ggcttcggta tacagctgct tgatggcgat cccttccgcg gtttgctgaa ccagcgagtc
    16981 gacagttttc tcccgacggc tcaattcctt gttggcaagc tgttgccact cctgctcgtt
    17041 agacattatt tgctccgtaa aacgcaatca aaatgagggt atttgcgctt acggtgagct
    17101 ttttacgtgg gtttgtcgcc agcaaaaaaa atgcattcag acgtcgtttt ttgccgcctg
    17161 agatttgtct attttgtgat gtgattcaag gaatacaaaa agaagtgagg ataacgcctg
    17221 atatgtgcta tcaggcatta tttgatggat taatcctgac gaaggacttt gtgaccataa
    17281 tcgagcaacg catcagtgac tttacgcatc atgcggcttt caggagcaaa gcggtgccag
    17341 tagagcatcc ggcgttgaaa tagccctggc gttaagtcaa tcagttcacc gctggctagt
    17401 tctttctcga tttgcaggtg cgggatcata cagcaggtgg tgccctggcg agcaagttgc
    17461 acgaacgctt ctgaagaatt aacgatatgg cagggcacgc tgcctggagg cagatcgaag
    17521 ttttgctgca aaaaggcctg gtgcatatcg tcaagatggt caaaggcgac cacaggtgct
    17581 ttcagtaatg ccgaacgcgt tacgccgtta gggaaatatt tttcggcaaa gggttttgag
    17641 ctgacgaaca gatagtcgag cgcaccaagt ttatcgacaa gacaactcgg cagcgcctga
    17701 tgttgaatac tcaccgcgcc gaccacttcg ccgcggcgca gacgttcctg agtacgggtt
    17761 tcatcttcta cctgcaagtt gaggcggata ggcgaatcag ccaacacagg agccagtgcc
    17821 ggaagcaacc acgtcgccag actgtcggcg ttgaccgcca gtgaaagcag cagcggagtc
    17881 gaaccggttt gttcatcgcc cagccactct tcttccagca actccacctg gcgcagcagt
    17941 gccagcagtt tttgcccttg ttccgtcggg cgcggcggta cggtacgcac caacagcggc
    18001 tgcccgaaca tattttccag ttgcttaatg cgctgtgaga cggctgattg tgtaatgcac
    18061 agcttttgtg ccgcgcgctc aaatcctcgt tcacgtatca ccgcatccag tgcctgtaat
    18121 gttctgtagt ccgggcgttt cattgctgtt gtctgctcct ggtaaatgct ttttcctgca
    18181 ctatgacaca attttgtgtc aggttgcata taccgaagcc gtcggcgggt aacatttgta
    18241 tccgtggtta aacgcgcagc ttaaaagatc agggatttgc gctaatgcat ttcgctcagc
    18301 gagttcgtgc cctggttgtg ttgaatgggg tggcgttatt gcctcaattt gcctgtaaac
    18361 aggggcttgc gaacgggggg aactggtgcg cctgttcgca ccgtggagcg gcatacccag
    18421 accgttgtat gctttatttg cggggcgaaa ggggatgccc gccattgcgc gatattttat
    18481 ggatgagtta accacgcggc ttgccaacgg ggtctgaatc gctttttttg tatataatgc
    18541 gtgtgaaatt tcataccaca ggcgaaacga tcatgacgca ggatgaattg aaaaaagcag
    18601 taggatgggc ggcacttcag tatgttcagc ccggcaccat tgttggtgta ggtacaggtt
    18661 ccaccgccgc acactttatt gacgcgctcg gtacaatgaa aggccagatt gaaggggccg
    18721 tttccagttc agatgcttcc actgaaaaac tgaaaagcct cggcattcac gtttttgatc
    18781 tcaacgaagt cgacagcctt ggcatctacg ttgatggcgc agatgaaatc aacggccaca
    18841 tgcaaatgat caaaggcggc ggcgcggcgc tgacccgtga aaaaatcatt gcttcggttg
    18901 cggaaaaatt tatctgtatt gctgacgctt ccaagcaggt tgatattctg ggcaaattcc
    18961 cgctgccagt agaagttatc ccgatggcac gtagtgcagt ggcgcgccag ctggtgaaac
    19021 tgggcggtcg tccggaatac cgtcagggcg tggtgaccga taacggcaac gtgatcctcg
    19081 acgtccacgg catggaaatc cttgacccga tagcgatgga aaacgccata aatgcgattc
    19141 ctggcgtggt gactgttggc ttgtttgcta accgtggcgc ggacgttgcg ctgattggca
    19201 cacctgacgg tgtcaaaacc attgtgaaat gatctgacgg gggaacctcc cccgttaaaa
    19261 aaattctctt cattaaattt ggtgacatgt gtcacgtttt tacttggcta ttgtcgattg
    19321 ctctaaataa atcctctaaa ccagcatatt caaccaagaa atacctttgc gtgatatttc
    19381 ctcaacatcg cgacgcaaac gttcatattg ccgcaatatt attttttgat atgttgaaag
    19441 gcggatgcaa atccgcacac aacatttcaa aagacaggat tgggtaaatg gcaaaggtat
    19501 cgctggagaa agacaagatt aagtttctgc tggtagaagg cgtgcaccaa aaggcgctgg
    19561 aaagccttcg tgcagctggt tacaccaaca tcgaatttca caaaggcgcg ctggatgatg
    19621 aacaattaaa agaatccatc cgcgatgccc acttcatcgg cctgcgatcc cgtacccatc
    19681 tgactgaaga cgtgatcaac gccgcagaaa aactggtcgc tattggctgt ttctgtatcg
    19741 gaacaaacca ggttgatctg gatgcggcgg caaagcgcgg gatcccggta tttaacgcac
    19801 cgttctcaaa tacgcgctct gttgcggagc tggtgattgg cgaactgctg ctgctattgc
    19861 gcggcgtgcc ggaagccaat gctaaagcgc accgtggcgt gtggaacaaa ctggcggcgg
    19921 gttcttttga agcgcgcggc aaaaagctgg gtatcatcgg ctacggtcat attggtacgc
    19981 aattgggcat tctggctgaa tcgctgggaa tgtatgttta cttttatgat attgaaaaca
    20041 aactgccgct gggcaacgcc actcaggtac agcatctttc tgacctgctg aatatgagcg
    20101 atgtggtgag tctgcatgta ccagagaatc cgtccaccaa aaatatgatg ggcgcgaaag
    20161 agatttcgct aatgaagccc ggctcgctgc tgattaatgc ctcgcgcggt actgtggtgg
    20221 atattccggc gctgtgtgat gcgctggcga gcaaacatct ggcgggggcg gcaatcgacg
    20281 tattcccgac ggaaccggcg accaatagcg atccatttac ctctccactg tgtgaattcg
    20341 acaacgtcct tctgacgcca cacattggcg gttcgactca ggaagcgcag gagaatatcg
    20401 gcctggaagt tgcgggtaaa ttgatcaagt attctgacaa tggctcaacg ctctctgcgg
    20461 tgaacttccc ggaagtctcg ctgccactgc atggtggtcg tcgtctgatg cacatccacg
    20521 aaaaccgtcc gggcgtgcta actgcgctga acaaaatctt cgccgagcag ggcgtcaaca
    20581 tcgccgcgca atatctacaa acttccgccc agatgggtta tgtagttatt gatattgaag
    20641 ctgacgaaga cgttgccgaa aaagcgctgc aggcaatgaa agctattccg ggtaccattc
    20701 gcgcccgtct gctgtactaa ttccccttct ctgaaaatta acgggcaggt cactgac
//
LOCUS       DAKISA010000081        20756 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.081, whole genome shotgun sequence.
ACCESSION   DAKISA010000081 DAKISA010000000
VERSION     DAKISA010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20756
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.081"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..183)
                     /locus_tag="OVW45_002960"
     CDS             complement(<1..183)
                     /locus_tag="OVW45_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5527359.1"
                     /translation="MTAFRAAFKAYRMHQVLILPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSA"
     gene            complement(225..683)
                     /locus_tag="OVW45_002961"
                     /pseudo
     CDS             complement(225..683)
                     /locus_tag="OVW45_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(684..785)
                     /locus_tag="OVW45_002962"
                     /pseudo
     CDS             complement(684..785)
                     /locus_tag="OVW45_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(969..2290)
                     /gene="hipA"
                     /locus_tag="OVW45_002963"
                     /pseudo
     CDS             complement(969..2290)
                     /gene="hipA"
                     /locus_tag="OVW45_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
     gene            complement(2290..2556)
                     /gene="hipB"
                     /locus_tag="OVW45_002964"
     CDS             complement(2290..2556)
                     /gene="hipB"
                     /locus_tag="OVW45_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HCV5527360.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDAKNASPEATEQQDLEW"
     gene            complement(2855..3100)
                     /locus_tag="OVW45_002965"
                     /pseudo
     CDS             complement(2855..3100)
                     /locus_tag="OVW45_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368382.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3102..6053)
                     /locus_tag="OVW45_002966"
                     /pseudo
     CDS             complement(3102..6053)
                     /locus_tag="OVW45_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001475310.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6207..7742
                     /gene="lsrA"
                     /locus_tag="OVW45_002967"
                     /pseudo
     CDS             6207..7742
                     /gene="lsrA"
                     /locus_tag="OVW45_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
     gene            7736..8044
                     /locus_tag="OVW45_002968"
     CDS             7736..8044
                     /locus_tag="OVW45_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCV5527361.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLMRKVVSAMISWIILSRARQKYGAASVVVDEQSWLSSGVKKSPY
                     "
     gene            complement(8216..9664)
                     /gene="uxaB"
                     /locus_tag="OVW45_002969"
     CDS             complement(8216..9664)
                     /gene="uxaB"
                     /locus_tag="OVW45_002969"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCV5527362.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFAL
                     AALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDHVIGTQELVAIVLAEKDHWEQ
                     DLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(9891..10841)
                     /locus_tag="OVW45_002970"
     CDS             complement(9891..10841)
                     /locus_tag="OVW45_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCV5527363.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGGFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPLTVMLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGL
                     VARMGGEEFAVAVPSVNPVDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRAS
                     YRTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(10980..11339)
                     /locus_tag="OVW45_002971"
     CDS             complement(10980..11339)
                     /locus_tag="OVW45_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCV5527364.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(11339..12265)
                     /gene="glsB"
                     /locus_tag="OVW45_002972"
     CDS             complement(11339..12265)
                     /gene="glsB"
                     /locus_tag="OVW45_002972"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCV5527365.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(12329..13717)
                     /gene="sad"
                     /locus_tag="OVW45_002973"
     CDS             complement(12329..13717)
                     /gene="sad"
                     /locus_tag="OVW45_002973"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HCV5527366.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALELAAAGFRD
                     WRETNIDYRAEKMRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENTLGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            13818..14699
                     /locus_tag="OVW45_002974"
     CDS             13818..14699
                     /locus_tag="OVW45_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCV5527367.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRTNCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQSYQ"
     gene            14776..15891
                     /gene="yneK"
                     /locus_tag="OVW45_002975"
     CDS             14776..15891
                     /gene="yneK"
                     /locus_tag="OVW45_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCV5527368.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFLNDFIEQF
                     PMFNPGVPIKIAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVLPEVCICFLFLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLELFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATSKTPDLPERH"
     gene            16041..17231
                     /gene="ydeA"
                     /locus_tag="OVW45_002976"
     CDS             16041..17231
                     /gene="ydeA"
                     /locus_tag="OVW45_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCV5527369.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(17256..17921)
                     /gene="marC"
                     /locus_tag="OVW45_002977"
     CDS             complement(17256..17921)
                     /gene="marC"
                     /locus_tag="OVW45_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCV5527370.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            18133..18567
                     /gene="marR"
                     /locus_tag="OVW45_002978"
     CDS             18133..18567
                     /gene="marR"
                     /locus_tag="OVW45_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCV5527371.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            18587..18970
                     /gene="marA"
                     /locus_tag="OVW45_002979"
     CDS             18587..18970
                     /gene="marA"
                     /locus_tag="OVW45_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCV5527372.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            19002..19220
                     /gene="marB"
                     /locus_tag="OVW45_002980"
     CDS             19002..19220
                     /gene="marB"
                     /locus_tag="OVW45_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCV5527373.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(19277..20716)
                     /locus_tag="OVW45_002981"
     CDS             complement(19277..20716)
                     /locus_tag="OVW45_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCV5527374.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
BASE COUNT         5326 a         5373 c         4674 g         5383 t
ORIGIN      
        1 ggcggataaa tcgacagatt cagcaagatc attgcttaaa aaccgtggat taaaataata
       61 ctctgcgtca acgggaaata ccgcagttgc taagccaagg gcaatggtaa gccgtgcaaa
      121 gcgaggcagt attaacacct gatgcatacg atatgcctta aatgcggcac gaaaggctgt
      181 cattattaat tccgattgag tttattatta attaggtcac tggcttactg catggttgcc
      241 ttaatacgcg gagttaatgc accgtaatca ttgatcgtct gaaaactgat atccccggtt
      301 gcagcatgcg ggatatccac cgatacttca ccttttggcg gcaccatggt gtttgggaga
      361 ttgctatttc ctgctttcat atttgttacg gtaatgaaat agggcgttgg attaataaga
      421 gtgagtttgc taccgctacg acgaaaccga agcattgcag gcgcttcttc aggtgccatt
      481 gccaggttcg tggggcgata aaacattttt atgcggctaa taattgccag ctgtaatgta
      541 ttctcattct tctggtcttt ttccatagca ggaatagctt taacattcac ccagaacagg
      601 ctttcacggt cgcctggtaa ctgatgatta gtagcgttga ttatccgcag cgtattctct
      661 tttttacctt gcttaagatt gaattataga tattgaactt tgaaggtcgc atcagcgttt
      721 gcagtaccag ctacggactg cccgagagca atataacgag cctggaatgg cagtatattc
      781 gtgccacgat aacatcttta aatcacaagg acacctaatc tgaacgaata acgactacca
      841 gaaaagcact tcgcatcact cagacatgat ttaacataat atacattatg cgcacaaaca
      901 taaaccaagg gagaatccag tcgttggccg gtcatgattg ctcattaaca acgaccaaac
      961 cccatatctc acttactacc gtattctcgg cttaaccgtc catgcaacct caacacattg
     1021 gtttcaactg ccgtcaccac gttctccgga aaatctgtcg gtaatgaagt cttcacgtta
     1081 tccagtgctg ccggaatcat tctggcaaag tcacttagga tttcatgcat ctgcacttcc
     1141 gggaatctca gcacctttgc tgtcgccaaa aaatgtcgcg gataaatttt atcgattgcc
     1201 gtttttttgc ctttggatgc gttaagcccc attgccagtt tgagatcgct gatgttattc
     1261 ccgtaccgcc aaggaccgga aatgctgaaa tgatgtcgta aaatggcgtg agtcgataac
     1321 tgccgccagc ctgaataaat acggagaagt tttttgcatg gccatccgtt gcgccaatca
     1381 accactggaa cacttgaaac ttcataaaat catagcgatc tttcagcgcc tcgctggacc
     1441 ccatcaaaaa cgccatgatc cgcgcgatgc ctgggcctcc atctgattca tatttcaccg
     1501 atgaaggtaa accgaatgtc tgacacatat cctcctgtgg caagcgaagt aaaaccgttc
     1561 gctcagtatt ccaacgcctg tcaaaacgtt cgaccgctaa cgcgcgcacc cttcccgctt
     1621 taatgatttc tgcgtccgga acattcaacc caagttcttt cgccagcagc agacagtaat
     1681 actcattatc aacgctttgg ctgagatcga gcgtcgcatt gggctgcctg atttcgccaa
     1741 tcggtaattt aatgatgtgc gtcgtcggcg ttattccttt cggaatgcac cagtcattgc
     1801 ctattctgag cagtgctgtc ttctcctgtg cgccagcaac cgagatgcga aagtcatttt
     1861 cttctctaat catgcctagc gggatatctg ctttataagc cgttaatact tcttcaagtc
     1921 tggcttcagt aagcttttcc catgccatta tcggacgcgt tacggtttcg tcttcgggta
     1981 ttaacgtcac ggcaccaacg ctgtctcgcc ctatttctga caacaaatca aacggttgtc
     2041 tggatttggc atgataacgt ttaacgatcc ggtcacgtac aatcgggcta tcgggtaaca
     2101 ggttatcgaa gaagttaaat acggcatcag aggtgatatt ccccctctgc aatggcagcg
     2161 aaagtgacaa cggtctggca taacggctgg ctaaccactc cggtgcatac ttaaaggtgt
     2221 gcgcgccgtt ggctaacttc gttaactcgc ctacccgctg gttgttcatc caagtgacaa
     2281 gtttaggcat taccactcca gatcttgctg ttctgttgct tctggcgagg catttttcgc
     2341 gtcgcatagc gtcattgaga gttcaagcga ctgtaaaatc ttaaaaaagg tcgtcagcgt
     2401 ggtattgtca gggttgtttt cgaaattgga aatcgtcgcc tgcttaatgc caattttttt
     2461 cgccagctcg ctctgcgtcc agccattttg ctggcgaacc agtttcattg cattcgccaa
     2521 ttgcgttggg ctatagatct tctgaaagct catcatgtca tacgtccaca gcaaatttat
     2581 ccgcttaagg ggatattatc agttttatcc tttagtgagg ataaagtcaa tctggaaaca
     2641 catcttatcc gctgtacggg ataaacgctg cgatatccgc gatcgcggat aaaagggagg
     2701 ctttaaaagc cttttgaagt tggcaatatt aagccgcatg ccatctcgac atgcggctta
     2761 tacggtttac cagctatatt tcactcccag catgccctga gtatcgctat agcctttatc
     2821 acctagttgc acaccgacat tcccccacag gctaatgctg attggcgtag cctgccatca
     2881 cgccaagatg ccagcgatcc tgcgcgttgc tactccactg cgccaaatct ccgcctagct
     2941 gcaatacata gcggttagcc tgagtattta gctggccgtc accggtcctg aaacgttcgt
     3001 gccccccgac atgacgcatc cacatactgc ttgccgatcc ctgagaatgc agtgaatctg
     3061 tatactgcgg ctcacccaga cggtcgtgta agcgatggct attttgccga catatcggcc
     3121 gttgcgccat aggctccgcc atgttcggct aacgtcagct tttgcccacc atggatgttc
     3181 agcgctttca gcccatagtt aacgtacaaa ccatcacctg gtgcggtcgt gaggcggaat
     3241 ccataatcac cctcggcgac caccgttccg ttttgcgcaa tgtgtaacgt tttgtccgct
     3301 tccacctcgt cgccctgtaa atcacgtaac gttaatgagc cacccgaccc aataaccgtt
     3361 tgcgccttca ccagttgaac gccgacatgg ctatcatcgt gttccagcaa attgagcgtc
     3421 gtcagagggt tattcgctat gggatcattc cacggtttag gcacgtcgat aagcacgtcg
     3481 cccgtcccgt ttacctgata gttacggcct ttccaggtgt agtcacccgc gccgacaacc
     3541 agcgtatcga cgctgatata tccctctgca agcgtcgcag caggaatatc cgtatcgaaa
     3601 atgagcgtac cgccattcat ggccagtcca ccaatggatt gctctcccac gtttaccgat
     3661 gtggtatttt cactgtcaga ctgcaacatc gcgtgagtaa gcgcagcggt gttgtcgcgt
     3721 tccagagtga aggttctgtc tttcagttgg gcaacaccgg cgaattcagt ccctgttgca
     3781 tgggtaaagc caaacatctt gtcgtaggat gacagctgca ctcgcatcaa gccatcgcct
     3841 ttgagcgcat tggtcagcgt gtaatctcct gcactgttcg ttgatgcgag aatgtcgagc
     3901 gtaccggggc ttgcaatggt gacttcactc gaacccatgc ccgttacccc atctgccgta
     3961 tgagacgcaa tggtcaggag gccgtcattg accagcgttt taccgctata agtattccct
     4021 gcattgaaca ccgtatggcc tgcatcatgg atcacttttc cgtctttatc gtcacctgta
     4081 atcagcatat cgacctggta gccggcatca ctgttattag tatgattaaa gacaaaaaca
     4141 ccttcgccag aaccaaactc tactttcgtc gcgttggtta tatacccggc atctgccgcc
     4201 gcctcaccgt gagctgcacc aatgtttagc gttcctacag caggttcaaa aacacctaag
     4261 taaacttctc caccttcgac attcagcgtg ccgctattcg tcagggttaa cgtacccgta
     4321 ccagatgtcc ctacgtacat attgaatgtt tcaagaagag agttctgccc atccaccctc
     4381 acgtcgccct tacttttgtc attgagagct atctgtgtat cacgcgcttt aactatacct
     4441 ccggtggtaa tattcagttc acccgtgcct aatacaccga cctgtagcaa ttgtgcgtta
     4501 gtagatgatg tctttaaatt ccagagactg tccgttgaaa tattaactat ccccttaccg
     4561 gaggcatttc ctccgataaa tccatagccc gttgaattaa gggtggcacc attactgaca
     4621 tttacgatac cgctaccaaa attaccgaca ttgataccca cagtatcgaa cgatgatgtc
     4681 gcatccatca ggttcagtgt gccgataccg cttgctttat cgcccaaaaa agtaatattt
     4741 tgcgatacaa acttaccgcc atcgctaata ttgaggtttc cgacgcccgc aacaccaatg
     4801 gtggtataga cattcttatt gacctcccac atcccccccg tggtgatgct gacattcccg
     4861 acgccgtttt ctccataacc gagtgagctt cctccgttgc tgacaacaag cccctcatta
     4921 ctgatattca tttcgccgtg gccgtcataa ccaagattag ttccgagatt agtgataacg
     4981 gagttcttat ccttaacagt aatgttgcct gtgcctgttt ctttcatccc cgcagtgata
     5041 attcccgaat ctactttacc ttcacgcgag acattaagtt caccgtcacc agcatcaccg
     5101 atatagatat agcggaaagc ttcgcctgtt ccgaggaaac tccaatgccc tttatcggtc
     5161 acgttgacga caccgtggga accgtcctga acgcccacca atgaatattc tttgttatta
     5221 atcagaccat tattggaaat attgactgtg ccattaccga aatacccatt atagagtctg
     5281 cgtaccgtga tgacagagtc ttgatcctgc acattcaggg ttccgatacc ggtggcattg
     5341 ccaccgataa tcgtattttc agccgtaatc agcccaccct cgcgaatagt cgcctcccca
     5401 gctccttgat taccaatttg aaattcaatt gaggaatcat tattttttat tagccactcg
     5461 ccaccctttt caacgataac cttaccatta ctgttcgctt gatatcctaa aatggcgact
     5521 attgaactcg tgacgtaacc cttatccgta atatttaatg agcccgtacc gtaactccct
     5581 atttcgaata attcggtcgt cagaacagag tcttctccct caacattgac cgtcccgacg
     5641 cctcctgtcg aggaacctag tcttaaatag cctccatcaa cgtgaccttt ctgtttaata
     5701 ttcagcgtcc ccgttccgga ttgacccaca tttaaaggcc ttgcattatt tccgctatca
     5761 tacaatcgcc aggtgccacc caaaacatta acggtaccct ccgaatcctc attagcgcca
     5821 atgacgctgg tagtaatagt ggttaagtag gcattaccgt cagccaaaat attaagaact
     5881 ccagtgccat accagccaac caggtaggca tcataagcaa catcagtatc aatattggta
     5941 atctgaccat tatcaacttc cagactcgca ccagacgctg aaccgtttag tgccagcaaa
     6001 acacccagcg tcaatctact gaatttcgtt gtcagtccat aggatttacg caaattaact
     6061 gttgatgtgc aaccgtgaaa atcaaaatag cataaattgt gatctattcg tcggaaatat
     6121 gtgcaatgcc cacctaaggt tatgaacaaa ttaaaagcag aaatacattt gttcaaaact
     6181 cacctgcaaa actgaacggg ggaaatatgc aaacgagtga tacccgcgcg ttaccgctac
     6241 tttgcgcccg ctcggtttat aaacagtatt caggggtcaa tgtcctgaaa ggcatcgatt
     6301 ttacgttgca tcaaggggag gtccacgccc tgctcggcgg caatggtgcc ggtaaatcga
     6361 cgttaatgaa gattattgcc ggtattaccc ctgctgatag cggtacgctg gagattgggg
     6421 gcaacaacta cgtcagatta acgccagttc atgctcatca gctgggtatt tatctcgttc
     6481 cccaggaacc gctgcttttc ccaagcctgt cgataaaaga aaacatcctg tttgggctgg
     6541 caaaaaaaca gctctccatg cagaaaatga agaacttgct ggcggcgctg ggctgctagt
     6601 ttgatctgca tagtctggca ggatcgctgg atgtcgccga tcgccaaatg gtggaaatcc
     6661 tccgcggcct gatgcgcgac tcgcggattc tgatcctcga tgaacctacc gcctcgctta
     6721 cccctgcgga aaccgaacgc ttgtttcgtc gcttgcaaga gctgcttgct actggcgtgg
     6781 gtattgtttt tatctcgcat aagctgccgg aaattcgcca gattgccgat cgaattagcg
     6841 tgatgcgcga cggaaccatc gccttaagcg gcaaaaccag cgaactgtct accgatgaca
     6901 ttattcaggc catcacgcca gtggtacggg aaaaatcgct ctctgccagc caaaaattat
     6961 ggctggagtt acctggcaac cgcccacaac atgccgccgg aacgtcggtg ctgacactag
     7021 aaaatctgac cggcgaaggt ttcaggaatg tcagcctgac gctcaatgcc ggagaaattc
     7081 tgggtctggc cgggctggtg ggggccggac gcacagaact ggccgagacg ctctatggtc
     7141 tgcgtacttt gcgtggcgga cgcattatgc tgaatggtaa agagatcaat aaattatcca
     7201 ctggagaacg tttactgcgc ggtctggttt atctgccgga agatcgccag tcatccggac
     7261 tgaatctcga tgcttcgctg gcctggaacg tctgcgccct tactcataac cttcgtggat
     7321 tctgggcgaa aaccgcgaaa gataatgcca ccctggaacg ttatcgtcgg gcgctgaata
     7381 ttaaatttaa ccaaccggaa caagctgcac ggacattatc cggtggcaac cagcaaaaaa
     7441 tcctgattgc caaatgcctg gaagcttcgc cgcaagcatt gattgtcgat gagccgacgc
     7501 gcggcgtgga tgtctcggcc cgtaatgata tctaccagct gttgcgcagc atcgccgcac
     7561 agaatgtggc agtgctgctt atctcctctg atctggaaga gatcgaactg atggcagatc
     7621 gtgtgtatgt gatgcatcag ggcgaaatta cccactctgc actgaccggg cgcgatatta
     7681 atgtcgagac tattatgcgc gttgccttcg gcgatagtca gcgtcaggag gcgtcatgct
     7741 gaagtttatt cagaacaacc gtgaaatcac ggcactgctg gcggtggtgc tgctgtttgt
     7801 attacccggt tttctcgacc gccagtattt aagtgtgcaa acgctgacca tggtttatag
     7861 cagcgcgcaa atcctgatcc tgctggcaat gggcgcgacg ctggtaatgc ttatgcgcaa
     7921 agtagtaagc gcgatgatat cttggataat actgagccgt gccagacaaa aatacggcgc
     7981 agccagtgtt gttgtggacg aacaatcatg gttatcttca ggcgtgaaaa agtcgcccta
     8041 ttaaacgtga ttacgatcac attctcaaga cgctacttac aaattaccta caagcttaca
     8101 acattaacag gcgctgcatg tggcatctgt taatgataaa ggcacatagg atgttgtaac
     8161 taatatagtg aaataagaaa cccggtcaaa accgggttca gaagtaacgg tgttattagc
     8221 acaacggacg tacagcttcg cgcatccctt tttcaagaat cgcatccagg tcattagcaa
     8281 cctgctcgac cagaccaggc acttgcgtca ggtcctgctc ccagtggtct ttttctgcca
     8341 acacaatcgc taccagttcc tgggtaccga taacatggtc acgatgctgg ctccagagtt
     8401 gctgataacg ttccagccag tgtgcatcgt cctgtaccgg atatgtttct ccgttacgct
     8461 caccacgata gaacgcaatc aatgccgcta atgcgaaagt aaggcgcgcc ggaagtgtgc
     8521 cgtttgcctt ctgccctgcc agcagctgcg gcaggatgcg ggtgcggaac ttggtcatac
     8581 cgttgagcgc gatagacagc agctgatgct tatacgggtt acggaaacgc ccggtgactg
     8641 cactggcgaa agattccagt tcatcacgag gcaaatccag taccggaata atttcttcgt
     8701 agatggcttt ttcaacgaat gcgcaaattt cagcatcgtt catcgcttca cctacggtat
     8761 ccagccctgc ctggaacgcc accggcacca gcgcggtgtg cgcaccgttg aggattgcca
     8821 ctttgcgttc tttatacggt ttaatatcgt caacaatcag cacgttgagc ggatatttgt
     8881 ccaggcgcag ctcagtcgct aaggatttcg gcccctgaat cacaaacagg taaaagtgtt
     8941 cagcggtgtc gaggaaacca tcgtgataac ccagttcttc ttccagttta gccacttcat
     9001 cgcgcggata accggtaacg atacggtcca ccagcgtaga acagaagctg ttagcctgat
     9061 ccagccattg aataaatgct tctggcagtg cccactcttg cgcatagcgc agcaccagtt
     9121 cacgcaacgc gtcgccattg tagtcgatca actcacacgg gatgatgatc caacctttat
     9181 ccagcgcacc gttgaaatgg ctaaagcgtt cgaacagcag acgggtcagt tttgccggat
     9241 agcttaccgc tggcgcgtca tcgaatttat cgcccgcatg gtagctgatc cccgcttcgg
     9301 tggtgttaga gaaaacaaag cgcatttccg ggttgtgcgc cagtttcagg aattcatcgt
     9361 attcactgta gacgctgatt tcacgattaa ccgagcgaat cagacgcgcg tcgctgaccg
     9421 cttccccctt ctcattcagg ccacggataa tggtggtata cagaccatcc tgcgtgctca
     9481 gtgacggcgg gaatgaagtt tcaatcggac gaacaacgac cacgccagaa ttcagatcgg
     9541 tgtgctcatt caggagatcg atttgccagt caacaaaggc gcgcaggaag ttaccttcac
     9601 caaactgaat gatacgttct ggatactgtg caccgggaaa atcgcgacga tttagtgttt
     9661 tcacaatggg tttccttctg attagtcata caacccgttt gaattggtac gacaggttag
     9721 caaactttaa tacgccgaac ccctgttttg atcaactcct gatgattaat gagcagtttt
     9781 atgagaaaag tgtggcgcgg atcatggttt aatcgaggaa atacgccttt tcctggatca
     9841 taaagtggta gaacacattg cattcaaatc gcgcggaatg aataaagatg tcagacaact
     9901 tcctcaccgt aacgcatagt gctggtacgg ttgcgcccat ctttcttcga gcgatacaga
     9961 cacgtatcgg cttcgaccat caatttatta aaatcatcgg tcagcgttcg gtacgatgcg
    10021 cgaccactac cgacgccaat acttaccgtg agataaagcg ttttttgttg ccaggtgaat
    10081 ggttgcagtt caacgccttt acggattttt tccgccatta gcagaccatc gacaggattc
    10141 accgacggca ctgcaacagc aaattcttcg ccgcccatgc gcgccaccag ccccttatca
    10201 ccgacaatct tctgaatatg ccgggcaaac acgcttaaca ctttatcgcc acattcatgt
    10261 ccatagttat cgttaatgct tttgaaatag tcgatatcaa gcagcatgac cgtcagaggc
    10321 acgatcaaca cgcccgttga gaattgctca ctgaaccagt ccgcaagcaa aggccagaaa
    10381 tccagactat caatactgac cgaaccaatc gccccgacaa tagcgcataa taatgcggca
    10441 atcagacagt aattaaatag ccgtaaggca cttactggct catacttatt tttccccaga
    10501 cgcttatccc gcacgaccag taaggcgacg gtaacaataa acatcatatt ggagaaattg
    10561 ataaccagtg aaaccagtcc ccattcggtg gttatggcat cataaaccag catcgccaca
    10621 taactgatcg catagtaatg cagtcgatta agccagacat agcgggcaaa cccccccgcc
    10681 attacgccat tcagcggcca gaaaagagaa agttcgtgaa ccagccgtaa ctctgcacca
    10741 ataaaataga acagcgtggt tagcgcaaaa atagcgatgc tattacgtag cagatgacct
    10801 tcctggaaca aacggaaagt tgaaatgggg tgtacatgca ttaattatct gtccatacaa
    10861 ttgcgggtgg caattatctc aacgtaataa gtaaattctc ataaaggata gcatgttcgc
    10921 taacagcggc ggcaaataaa ggtaccctgg gaatgctatt ttatcgtttt ccgcaattct
    10981 catggctgat tcatctgtac aaccagccag tgatagatta ccgccacgat ataacgttgt
    11041 tgttcttcgc ttaacgaaac accctgcgga atgttatgcc acttagccag acaaccgcga
    11101 cagcaggtcg cggtggcgtg ctgagcgata aacaccggat gcccgcgcat gggggtttgc
    11161 ttaccgtcat tagccggtaa tgccggagca agacgtttag cgacaaaatc cgccgcatgt
    11221 tgttcgatga ctggcgcgcc tttctccagg caatactgac gctctttcat gcccagacga
    11281 aagcgagagc gaaattttga acgggataaa cgcgcgaaga gtggatcgag agactgcatt
    11341 aataaaccga acgccctaac tgtttcgtca attgttcaag gacggcgata cccgcaagcg
    11401 agttacctgc atcatccagt tccggactcc agactgcgat ggccatttca tgcggaacaa
    11461 tcgccacaat accgccacca acgccagatt tcgccggtag tcccacccgc caggcaaact
    11521 cccccgcgtt ctggtacata ccactggtcg ccatcagcgc gttaatttgc cgcgcctgca
    11581 ttggcgtcac tacaggttca tcaatatgaa tagctttccc ctgattagcc agaaagacaa
    11641 acgtccgagc cagctctaca cagctcattt tcagagcgca gtaatgaaag tagttttgca
    11701 gaacggttgt cacgtcatga tggaaattgc caaacgactt catcagccag gcgatagccg
    11761 catttcgcgc ggaatgttca aattcggaac gcgctaccac cgtatcgtat gaaatatcag
    11821 acacaccgct taagccgcgc acgacttcca gcatacgttg ccgtggtgcg cttaatcgcc
    11881 cttgcaacat atcgcagacc accagcgcac cagcattaat aaacggatta cgcggaatac
    11941 cctgttccat ttccagttgc actaaagaat tgaacggtga tccagacgga tctttgccga
    12001 cgcgttgcca gatttcctct tcggagtaat gacgcatggc gacaacgaga ctcagcactt
    12061 tggaaataga ctgaatggaa aaacgttctt gcgcgtctcc ggcctgaaaa agctgtccgt
    12121 caacggtaca gatagcaatg cccaatcggg aaccgtctac tgtagccagc gccggaatat
    12181 aatccgcgac tttaccctga ccaatgagcg gccgcacttg ccgcaagatg ttttctaaaa
    12241 ttgcattatc catggcgact gccactttct actcctggac cgcaggtctg aaaagacctg
    12301 cgagtatatc agagctgaat atgtcgcgtc agatccggtc tttccacacc gtctggatat
    12361 tacagaattc gtgtaagccg aaatgggaaa gctcacgacc aaagccactc tttttcacgc
    12421 caccaaaggc cactcgcgcg tcgctggcac aataaccatt gataaacacc ccaccgcatt
    12481 ccagacgtgc cgccatctgt ctggcctgtg tttcgtcggt ggtaaaaatg gtcgctgaaa
    12541 ggccgaactc actatcatta gccagttcca gcgcatgttc tgcatctttc gcaacggtga
    12601 ttgccgcaac ggggccaaac atttcttccc gaaacgcggt catttctggg gtaacattcg
    12661 ccagaaccgt tggcggatag tagttacctg ccccagccat cttttccccg cccagtaaca
    12721 aacgcgcacc ctgcgccagg gttttctcta cctgatgatg cagctcatca cgtaaatcaa
    12781 aacgagccat tggtccgaga gtgttctctt cgtcacgggg atcgcccatt ttcaaggctg
    12841 ccgcagctgc cacaaaacgt tcggtaaatg cagaagcaat tccctcttcg ataataaagc
    12901 gttttgccgc tgcacatacc tgtccggtat tctgataacg tccggctacc gccgctttaa
    12961 ccgccagttc cagatcggca tcgttaagca caataaacgg atccgaaccg cccagttcca
    13021 gtacgcattt tttcagtgcc gctccagcct gtgcgccaat agccgctcca gcacgaacac
    13081 ttccggtcac cgtgacagca gcaatgcgcg agtctttaat catctgactg acaccgtcgt
    13141 tgtcggcatt cagccagcca tatacgcctt gcgggattcc cgcatcttta aacacctggg
    13201 caatgagctg agcacatccc atcacattcg gcgcatgttt aagtaagtag ccgttacctg
    13261 caagaatgat gggaacagcg ccacgcatca cctgccataa cggaaaattc cacggcataa
    13321 tcgccagaat cgtccccaac ggtcgatact caataaccgc ctgctgattt tccaccagtg
    13381 taggttccgc cttcagcatt gccggaccat gttctgcata ccagtcacac aaattcgccg
    13441 atttcgccac ttcagcgcgc gcctggttga ttggtttgcc catttcgcgg gtgatcattt
    13501 gcgccatttc ttcgctacgg gcgcgcagag ccttaccgat atcacgcatt ttttcagcac
    13561 gataatctat atttgtctcg cgccagtcgc gaaagcctgc tgccgccagc tcaagtgcgt
    13621 tttcgatatc gttagcgcca gcccacggca gcacagaaag ttgttcaccc gtggcaggat
    13681 ttattgaaat tgcatgagtt gccggagtaa tggtcatcgg ggtatctcct ttatgagtca
    13741 tggtatgaag atacgcagat ttactcttgc tttaaaatga ataatattaa gccacttatt
    13801 cacgaatcga gaatgttatg gatctgaccc aactggagat gttcaacgcg gttgccgaag
    13861 ctggcagcat tacccaggct gcagcaaaag tgcatcgcgt accgtcgaat ctcactaccc
    13921 gtctacgcca gctggaaaca gaactggggg tagatctgtt tattcgcgag aatcagcgtt
    13981 tacgtctctc tcctgccggg cataactttt tacgctatag ccaacaaatt ctcgcgttag
    14041 tggatgaagc gcggagcgtt gtcgctggcg atgagccgca aggtttattt tctcttggtt
    14101 cgctggaaag caccgcagca gtgcgcattc cggccacgct ggcggaattc aaccatcgtt
    14161 atcccaaaat tcagttttca ctttccaccg gcccttccgg cactatgctg gaaggagtgc
    14221 tggagggaaa actgaatgcg gcatttattg atgggcccat taaccatact gccattgacg
    14281 ggataccggt ataccgcgag gagctgatga tcgtcacgcc acaaggtcat gcgccagtaa
    14341 tccgcgccag tcaggttaac ggcagtaaca tttatgcctt ccgcaccaat tgttcgtatc
    14401 gtcgccactt cgagagctgg tttcatgctg acggtgccgc tccgggaact attcacgaga
    14461 tggagtcgta tcacgggatg ttggcctgtg tggtcgcagg agcaggcatt gcgcttattc
    14521 cacgctctat gctggaaagt atgccggggc atcaccaggt tgaagcgtgg ccgttagctg
    14581 agcaatggcg ttggttaaca acctggctgg tctggcgtcg cggtgcgaaa acgcgtccgc
    14641 ttgaggcatt tattcaacta ctggatgtgt ctgactcggc aaaacagagt taccaatgaa
    14701 ctattttgat agttcttgcg gctaatatgc tctatatagt gatgttccga tgacttatga
    14761 ctatatgggg caaatatggt tacgccagta agcatcagca attacatatc tcttcctgat
    14821 gattttcccg ttcgtaatat tgcaccacag gtaaaagaag ttttaaagga ctttattgat
    14881 gcgctaagta caataatatg tgatgaagag tggcgcacgt ctttaaacat caactcagcc
    14941 acaaaaaaga tatttaataa ccttgataac ttatcatata ttcagaggac ttctttccgg
    15001 ggtaacgaca cgctatacaa tgaaaaggtt cagtttaaac tcacttatcc cgtcaagaat
    15061 ggaagacaca aagaaaatat tgaatttcag gtagtaataa atttaagccc tatttattta
    15121 gataattttc gccatgatgg agaaattaat attttttgcg ctcccaaccc aaagcctgtc
    15181 actatggggc gcgtatttca gaccggcgtc gagcgtgtgc tctttctgtt tctgaatgat
    15241 tttattgaac aatttccaat gttcaaccct ggcgttccga tcaaaatagc gcatacacca
    15301 catattgaac ccctgcctcc ggatcaccat accgctgcag attacttacg ccagtttgat
    15361 ttgcttgtcc tgaattttat ctctcgcggt aattttgtca tactcccccg attatggaat
    15421 aactctgagg ttcacagatg gttcgtcaat aaagatccta acttgatcac cgccattctc
    15481 gacataacgg acagcgaatt aaaagaggat ttattgcaaa gcctgatgga ttcattaggt
    15541 tctaacaaac atgtactacc cgaagtctgc atctgctttt tattcctttt agcagaacaa
    15601 gaatctcctc attttcagga cttgttttta tttttcgcca atatgttatt gcactatcac
    15661 caatttatga atcccaatga aagtgatttg aatgacgtgt tgatgccagc atcattaagt
    15721 gatgataaaa ttatcaagca tatggcgcgc aggaccctcg aactgtttgt aaaaaatgaa
    15781 acaccgccaa aagttactca cgaagacctg gtgaaaaaca ggcctcgctc ccctgtcaga
    15841 ccacctatac ccgcaacctc caaaacgcca gacctccctg aacgtcatta aaccgtgatg
    15901 ttaccgactc tctgacgcgg aacagaatca gcgtcagaga aacggaaaac gcgatccaga
    15961 tcacaaatac attgtattca catcattaac cgttttaaga tcatttcatc actttttcgc
    16021 aactcacccg ataatctgtt atgacaacaa acactgtttc ccgcaaagtg gcgtggctac
    16081 gggtcgttac gctggcagtc gccgccttca tcttcaacac caccgaattt gtccctgttg
    16141 gcctgctctc tgacattgcg caaagttttc acatgcaaac cgctcaggtc ggcatcatgt
    16201 tgaccattta cgcatgggta gtagcgctaa tgtcattgcc ttttatgtta atgaccagtc
    16261 aggttgaacg gcgcaaatta ctgatctgcc tgtttgtggt gtttattgcc agccacgtac
    16321 tgtcgttttt atcgtggagc tttaccgttc tggtgatcag tcgcattggt gtggcttttg
    16381 cacatgcgat tttctggtcg attacggcgt ctctggcgat ccgtatggct ccggccggga
    16441 agcgggcaca ggccttgagt ttaattgcca ccggtacagc actggcgatg gtcttaggtt
    16501 tacctctcgg gcgcattgtg gggcagtatt tcggttggcg aatgaccttc ttcgcgattg
    16561 gtattggggc gcttatcacc cttttgtgcc tgattaagtt acttccctta ctgcccagtg
    16621 agcattccgg ttcattgaaa agcctcccgc tattgttccg ccgcccggca ttgatgagca
    16681 tttatttgtt aactgtggtg gttgtcaccg cccattacac ggcatacagc tatatcgagc
    16741 cttttgtgca aaacattgcg ggattcagcg ccaactttgc cacggcatta ctgttattac
    16801 tcggtggtgc gggcattatt ggcagcgtga ttttcggtaa actgggtaat cagtatgcgt
    16861 ctgcgttggt gagtacggcg attgcgctgt tgctggtgtg tctggcactg ctgctacctg
    16921 cggcgaacag tgaaatacac ctcggggtgc tgagtatttt ctgggggatc gcgatgatga
    16981 tcatcgggct tggtatgcag gttaaagtgc tggcgctggc accagatgct accgacgtcg
    17041 cgatggcgct attctccggc atatttaata ttggaatcgg ggcgggtgcg ttggtaggta
    17101 atcaggtgag tttgcactgg tcaatgtcga tgattggtta tgtgggcgcg gtgcctgctt
    17161 ttgccgcgtt aatatggtca atcattatat ttcgccgctg gccagtgaca ctcgaagaac
    17221 agacgcaata gttgaaaggc ccattcgggc cttttttaat ggtacgtttt aatgatttcc
    17281 aggatgccgt taataataaa ctgcacaccc atacatacca gcaggaatcc catcagacgg
    17341 gagatcgctt caatgccacc cttgcccacc agccgcataa ttgcgccgga gctgcgtagg
    17401 cttccccaca aaataaccgc caccaggaaa aagatcagcg gcggcgcaac catcagtacc
    17461 caatcagcga aggttgaact ctgacgcact gtggacgccg agctaataat catcgctatg
    17521 gttcccggac cagcggtact tggcattgct agcggcacaa aggcaatatt ggcactgggt
    17581 tcatcttcca gctcttccga cttgcttttc gcctccggtg aatcaatcgc tttctgttgc
    17641 ggaaagagca tccgaaaacc gataaacgcg acgattaagc cgcctgcaat tcgcagaccg
    17701 ggaatcgaaa tgccaaatgt atccatcacc agttgcccgg cgtaatacgc caccatcatg
    17761 atggcaaata cgtacaccga ggccatcaac gactgacgat tacgttcggc actgttcatg
    17821 ttgcctgcca ggccaagaaa tagcgcgaca gttgttaatg ggttagctaa cggcagcaac
    17881 accaccagcc ccaggccaat tgctttaaac aaatctaaca ttggtggttg ttatcctgtg
    17941 tatctgggtt atcagcgaaa agtataaggg gtaaacaagg ataaagtgtc actctttagc
    18001 taaccttgca tcgcattgac caaaacttga accgatttag caaaacgtgg catcggtcaa
    18061 ttcattcatt tgacttatac ttgcctgggc aatattatcc tctgcaacta attacttgcc
    18121 agggcaacta atgtgaaaag taccagcgat ctgttcaatg aaattattcc attgggtcgc
    18181 ttaatccata tggttaatca gaagaaagat cgtctgctta acgagtatct gtctccgctg
    18241 gatattaccg cggcacagtt taaggtgctc tgctctatcc gctgcgcggc gtgtattact
    18301 ccggttgaac tgaaaaaagt gttgtcggtc gacctgggag cactgacccg tatgctggat
    18361 cgcctggtct gtaaaggctg ggttgaaagg ttgccgaacc cgaatgataa gcgcggcgta
    18421 ctggtaaaac ttaccaccag cggcgcggca atatgcgaac aatgccatca attagttggc
    18481 caggacctgc atcaagaatt aacaaaaaac ctgacggcgg acgaagttgc aacacttgag
    18541 catttgctta agaaagtcct gccgtaaaca aaaaagaggt atgacgatgt ccagacgcaa
    18601 tactgacgct attaccattc atagcatttt ggactggatc gaggacaacc tggaatcgcc
    18661 actgtcactg gagaaagtgt cagagcgttc gggttactcc aaatggcacc tgcaacggat
    18721 gtttaaaaaa gaaaccggtc attcattagg ccaatacatc cgcagccgta agatgacgga
    18781 aatcgcgcaa aagctgaagg aaagtaacga gccgatactc tatctggcag aacgatatgg
    18841 cttcgagtcg caacaaactc tgacccgaac cttcaaaaat tactttgatg ttccgccgca
    18901 taaataccgg atgaccaata tgcagggcga atcgcgcttt ttacatccat taaatcatta
    18961 caatagctag ttgataacgt gacaacgtca ctgaggcaat catgaaacca ctttcatccg
    19021 caatagcagc tgcgcttatt ctcttttccg cgcagggcgt tgcggaacaa accacgcagc
    19081 cagttgttac ttcttgtgcc aatgtcgtgg ttgttccccc atcgcaggaa caaccaccgt
    19141 ttgatttaaa tcacatgggt actggcagtg ataagtcgga tgcgctcggc gtgccctatt
    19201 ataatcaaca cgctatgtag ttattgtcag gctctgatca cagagcctga catctccgta
    19261 atatcttcct caagtctcaa atattttcgc cattgttggc gatgagatcc cgataccagt
    19321 aaaacgacgc tttacgtatc cgttccagcg ttcccttccc ttcgttgtct ttatcgacat
    19381 aaatcattcc gtagcgcttt ttcatttccc ctgttccggc agaaaccagg tcaatgcagc
    19441 cccacggggt gtagccaatt aagtcaacac catcttcaac tacggctttt ttcatttccc
    19501 gaatatggga agcaaagtaa tcaatgcgat agtgatcgtt caccgtgccg tcagcttgtc
    19561 tctggtcaac cgcaccaaat ccattttcga caataaacag cggcaactgg aaatgatccc
    19621 agaaccagtt gagtgaataa cgtagccctg ccggatcaat ttgccatccc cattcggatg
    19681 tatcgatata agggttgctg accaaatctc gtggttcaac ataatccagt tgcgggttat
    19741 cgggagaaaa ttgtgtcgta aaagacatgt agtagctaaa accgataaag tcgacacatc
    19801 cactggcaag aatcgcgtta tcttctggtg tgatatcgag gttgaatcct ttcctggcaa
    19861 agtaattcag catatgttgc ggataatatc cacgagcatg aacatcagta aaccagtaac
    19921 gacgatgcat cgctttcgtg gccatcatca tatcgttggg tgcacacgtc agaggataga
    19981 tggggcacat ggcgatcata cagccaatgt taaattcagg attgatcgat tttccagtct
    20041 gtaccgccag ggcactggca actaactcgt aatgtaccgc ctggtacatt atttgttcgc
    20101 gctcattgat atcttcctct ggcgaataca agataccgga attagtaaat ggacacaggc
    20161 tttcgctgaa attcacctga ttattgattt cgttaaacgt catccagtac tttacttttt
    20221 ctttatagcg cgtgaagacc gttgatgcga agcggataaa aaagtcgatc agcttacggt
    20281 ttcgccagcc accatatttt gtcaccagat gataaggcat ctcaaaatgc gaaagcgtca
    20341 ccacaggttc cattccttgc ttcaggcatt catcgaacag atcatcataa aattgtaaac
    20401 cctcttcatt cggctcctgt tcgtcacctt gcggaaagat tcgtgtccag gcaatggaag
    20461 ttcgaaagca tttgaatccc atctcggcaa ataactgaat atctgtttta tagcgatgat
    20521 aaaaatcaat tgcttcatga ttgggataat taagcccgtc gataacgcct tctgtcactt
    20581 cacgcggcac cccgtgagcg ccagcagtca tcacatcagc gatactgatg ccttttcctc
    20641 cttcattcca gccaccttcc aactgatgcg cggctaccgc gccaccccat aaaaaacctt
    20701 ttttaaatcc tgacatacac aactccttaa ctaaaataga ttaccaccag acttcc
//
LOCUS       DAKISA010000082        20324 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.082, whole genome shotgun sequence.
ACCESSION   DAKISA010000082 DAKISA010000000
VERSION     DAKISA010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20324
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.082"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            121..675
                     /locus_tag="OVW45_002982"
     CDS             121..675
                     /locus_tag="OVW45_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCV5527375.1"
                     /translation="MNKKSMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(685..2112)
                     /locus_tag="OVW45_002983"
     CDS             complement(685..2112)
                     /locus_tag="OVW45_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCV5527376.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(2109..2816)
                     /locus_tag="OVW45_002984"
     CDS             complement(2109..2816)
                     /locus_tag="OVW45_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCV5527377.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPALSWQP
                     LADYPTCPETLKDMSDL"
     gene            2980..3957
                     /locus_tag="OVW45_002985"
     CDS             2980..3957
                     /locus_tag="OVW45_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCV5527378.1"
                     /translation="MVFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(4027..4509)
                     /locus_tag="OVW45_002986"
     CDS             complement(4027..4509)
                     /locus_tag="OVW45_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCV5527379.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            4744..7326
                     /gene="leuS"
                     /locus_tag="OVW45_002987"
     CDS             4744..7326
                     /gene="leuS"
                     /locus_tag="OVW45_002987"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCV5527380.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNMLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7341..7922
                     /gene="lptE"
                     /locus_tag="OVW45_002988"
     CDS             7341..7922
                     /gene="lptE"
                     /locus_tag="OVW45_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCV5527381.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7922..8953
                     /gene="holA"
                     /locus_tag="OVW45_002989"
     CDS             7922..8953
                     /gene="holA"
                     /locus_tag="OVW45_002989"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCV5527382.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELKGLSLLLCHK
                     PLADVFIDG"
     gene            8955..9596
                     /gene="nadD"
                     /locus_tag="OVW45_002990"
     CDS             8955..9596
                     /gene="nadD"
                     /locus_tag="OVW45_002990"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCV5527383.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            9620..10231
                     /locus_tag="OVW45_002991"
     CDS             9620..10231
                     /locus_tag="OVW45_002991"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCV5527384.1"
                     /translation="MRLWLIRHGETQANIDGLYSGHAPTPLTARGIEQAQNLHTLLHG
                     VSFDLVLCSELERAQHTARLVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEFQHYQNILVVSHQGVLSLLIARL
                     IGMPAEAMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            10491..10808
                     /gene="rsfS"
                     /locus_tag="OVW45_002992"
                     /gene_synonym="rsfA"
     CDS             10491..10808
                     /gene="rsfS"
                     /locus_tag="OVW45_002992"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCV5527385.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10812..11279
                     /gene="rlmH"
                     /locus_tag="OVW45_002993"
     CDS             10812..11279
                     /gene="rlmH"
                     /locus_tag="OVW45_002993"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCV5527386.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11310..13211
                     /gene="mrdA"
                     /locus_tag="OVW45_002994"
     CDS             11310..13211
                     /gene="mrdA"
                     /locus_tag="OVW45_002994"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCV5527387.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNAPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            13214..14326
                     /gene="mrdB"
                     /locus_tag="OVW45_002995"
                     /gene_synonym="rodA"
     CDS             13214..14326
                     /gene="mrdB"
                     /locus_tag="OVW45_002995"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCV5527388.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14337..15425
                     /gene="rlpA"
                     /locus_tag="OVW45_002996"
     CDS             14337..15425
                     /gene="rlpA"
                     /locus_tag="OVW45_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCV5527389.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15564..16775
                     /gene="dacA"
                     /locus_tag="OVW45_002997"
     CDS             15564..16775
                     /gene="dacA"
                     /locus_tag="OVW45_002997"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCV5527390.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16885..17148
                     /locus_tag="OVW45_002998"
     CDS             16885..17148
                     /locus_tag="OVW45_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCV5527391.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            17249..17890
                     /gene="lipB"
                     /locus_tag="OVW45_002999"
     CDS             17249..17890
                     /gene="lipB"
                     /locus_tag="OVW45_002999"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCV5527392.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            18150..19103
                     /locus_tag="OVW45_003000"
     CDS             18150..19103
                     /locus_tag="OVW45_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCV5527393.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            19312..20277
                     /gene="lipA"
                     /locus_tag="OVW45_003001"
     CDS             19312..20277
                     /gene="lipA"
                     /locus_tag="OVW45_003001"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCV5527394.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
BASE COUNT         5194 a         5085 c         5095 g         4950 t
ORIGIN      
        1 attgttcttc cttattcatt ttttaataat cccttaagtg cattaactga ctactttttt
       61 attattgatg agtataaact tgacgctttc aaaataaaaa gaaaatcgaa gcattcacac
      121 atgaataaaa aatcaatgta tatattcgca atttttatag ttgcagcaat tacctgtatt
      181 agccaaccca agaaaacgac gttgcgtgat aaagccatgg tgaattatgc ctttgattat
      241 ttaagatcac caggcagtct accgttaacc acggcagcca cggagctttc cgcgattcat
      301 ggtcactcaa catcgcaata tcgccttggc gaattttatc ttcatggtag cgacggtaaa
      361 ccactggatt atacacaggc gagatactgg tatgagcaat cagcagaaca ggaaaatccg
      421 cgcgcacaaa gcaaactggg gtggatctac ctgaaaggcc tgggagtcaa acccgatacc
      481 cgtaaagcaa ttctctggta taaggaagca gctgaacaag ggtatgctca tgctcaatat
      541 actttgggtt tgatctacag aaatggcact ggtattaatg ttaaccatta tgaatctcaa
      601 aaatggttaa aactggccgc caaacaacat tacaaaaatg cggaaagatt acttgccgga
      661 cttcccgcac attaacgtcg actgtcaccc gaagagatgg agtatttttc ctatcaaacc
      721 ggcaaagata aaaatgtaga aaataatttt gatgacgccc agacagccca gcccgccatc
      781 gtcaacgggc attggctgcg gttgcggctc cggctcgacg gtttcctgac cggtaaaatt
      841 cgccgcctga attaataatc gacgaataat taatgcttcc agtggacaac gatcttctgc
      901 atcaaggcgg ctcacaatcg gcattaatga ttcttctgtc ggttggctgg cctgcactaa
      961 ttgtttggca actaaatata atggccataa gggatgttct gtatcacgcg tgagtgagat
     1021 ccgcggctta cgtcctttgt gcaaaaccat ctcgaccagt gaacgcccca gcgcagagag
     1081 tttttcatcg ctgagtattt cgtataaatg aaaacgcgta cgggcgctgt cgccggggac
     1141 ataaagtagc tgtttaccct gatcgttaac cagttcacga gcttcaatca tcatcaccac
     1201 taacgggtaa taatcgaccc gatgctgacg gcaccattgc aatattaaag catccgcctg
     1261 ggtggaggga tactcgcgcc agtagtcaca aagttctgcc agcaagcttt cgccttctcc
     1321 acaatagacg cgttgcgcgt attcataata acgcgcatct tcattatcag gttcagcttc
     1381 ccgccacgac cgggcgtaat ccagaagttc ggggatatcc aggcgacctg cgctatacca
     1441 gcggagtaaa tttctgtgca gttttgcatc atcggggatg atcactgcgc gatgttgcgc
     1501 taaatacaat gtcagccatt ctggatgcga tgaccaaatc cgagcgaaca tttggtaaaa
     1561 atcgatggtg tcattttgca cggcaacggg cagatgcaga atggtctgga agttaaaaac
     1621 attccccttc gcaatggcat ataaaaagga ttccagttct tcttcgtcta tttccccgac
     1681 gttttcctcc tgcaaccacc gcaatctttc tgctaataac accacgcact cgaaggataa
     1741 atgggcagtg ttcatggcga ttgtgcacag cgaccagcgt aattcatcaa tctcttccat
     1801 cgagcaataa tttaattgct gaatgaatcg ctgccaggcg gagggcagaa aacgttcgct
     1861 atcagaggca agtaacgcgc gaaaggcgag cagaatttca tgttctgcta cctcatattc
     1921 ttctggttgc caaacagatt tagcaggcga ctgcgcaatc cggagcgcct cctcatacgc
     1981 ctgacgaagt tgtttaaaac cctgcggatc ggtttctgga tgaaaggacg gtaacagcgc
     2041 cagataagcg cggcggataa tatcgacgtc agtcgtttcc tctatttcga ggatcttcca
     2101 gcaatttttc ataggtcgct catatcctta agcgtttccg ggcatgtggg gtagtcagcc
     2161 agtggttgcc aggacaaggc agggtaatac gcatcatcac tggcaaataa ttgataaaac
     2221 atatcattgc cggaatcgtc gtattcttta cgagctaaga attcgcgtaa taaaggcaga
     2281 tgatcatctt cgggagactg ccaatattgc cgaccaaagg tataaccggc aaagtattgc
     2341 cgccagttac tataataacg tagagcccgt agatgaatac gcgactggat ccatagactt
     2401 tcttcttcac tcaaccagtt attaagtacg cccattcgac ttaaaaaacc catccggaca
     2461 taatcccatg ctttaattcc tccctcacca cagcattcgg ctgtgctggc gacaaattgc
     2521 gcataagcct gaccttgctc atttagttca gcaagatagt cacgccattc acatggcgaa
     2581 taacgaaacc agcggtgata aaaccctgcc aaccgagcgg catgaccgtt atcgatcatt
     2641 cggtgaattg tgcgcagcag gtcatcacgg tcatcaattc cccatgagga agaaaggtca
     2701 aaaaagcatg tacctaaata cattttgggg gatgaatgcg aaggtaaaca gcattcattg
     2761 cgtatttcca tcggagtgga aagtgcaaac aatatttttt gcgaccccat gtccatataa
     2821 tttatcctta aacattcatt taatgaatgt aaactacatt tcgagactca gttatttata
     2881 cttttctgac gcaacataat acgcataaaa taaggcttac gcaaaaatgg aacacataaa
     2941 aatataatat gttttatcta caaccataag gaaatgataa tggtttttac gtcaagttgc
     3001 tgcgataatt tatcaataga tgagattatc gaacgtgctg aaaaaggcga ttgcgaggct
     3061 caatatattg ttgggtttta ttataatcgc gatagcgcaa ttgactctcc ggacgacgaa
     3121 aaggcctttt actggctgaa gctggccgct gagcaaggtc attgtgaagc acagtattcc
     3181 ttagggcaaa agtataccga ggataaaagc cgtcataaag ataatgagca agccatcttc
     3241 tggctgaaaa aagctgccct acaaggccat actttcgctt ccaacgccct tggctggaca
     3301 ctggatcgtg gagaagcccc caattataaa gaagcggttg tctggtatca gatagccgcg
     3361 gagagcggaa tgtcttatgc gcaaaataat cttgggtgga tgtacagaaa tggcaacgga
     3421 gtcgcaaaag actatgcgct ggcatttttc tggtacaaac aagctgcatt acaaggccat
     3481 agtgacgcgc aaaacaatct ggccgatctt tatgaagacg gaaaaggcgt tgctcaaaac
     3541 aagacactcg ccgcattctg gtatttgaaa agcgcacagc agggtaatcg gcacgcccag
     3601 tttcaaattg catgggatta taacgctggc gaaggggtgg accaggacta taagcaagcg
     3661 atgtactggt atctgaaggc tgccgctcag gaaagcgtcg gcgcttacgt caacatcggt
     3721 tatatgtata aacacggaca aggcgttgag aaagattatc aggctgcctt tgaatggttt
     3781 acgaaagccg ctgaatgcaa tgacgccact gcctggtata acctggccat tatgtatcac
     3841 tacggagaag gaagacctgt cgatctccga caggctctcg acctgtatcg taaagttcag
     3901 tcatccggaa ccagagatgt cagtcaggaa attcgtgaga ctgaagattt actgtagaga
     3961 taaaaacaag ggccggggaa tttcattcgg cccatattct ctttattgct gtgcgaaatc
     4021 agactattac ggctcaaacg ggcgtctctg gaactggtca tgaccacatt tcgggcatag
     4081 cgtcagcact tccggtgtgt agatcgggag atggaagtga catttctcgc agaccagatt
     4141 tcccagcccg accacttctc cgctgtgata aaccccatga tgattgaggt cctggaaaac
     4201 ttcgcgccat tcaagctgcg ttttatcggt gatatctgcc agctcctgcc ataagctttc
     4261 tttaatcacc cgcataaaga cgctgtcaga ttcttctttc aggctctctt catagctcat
     4321 ggcgaactct tccaggtcac gtcttacagc tcgcgtcagc tcatcgacct cggttcgcgt
     4381 taactcccct gtttttatta cgcgctcgcg cgcctgttcc accagtgcgt cgatatcacg
     4441 ttcgccattg cgcaggcgtt cgctcagtga cgcgaccagt tcacggtaat attgagcaac
     4501 cttgttcatc gtttcatctc ccgggtcgtt aactgactat aataatagac gttctttgtt
     4561 ccttgctttg ctaatacgcc cacaaactct gtaaattaat gtatgaataa tccgggcatc
     4621 atttcaacgt cgagtgcgcg aaaggctgtt ttgacgcgga cgtttgggct atgctatgcg
     4681 gatctgaaaa accacctcaa cgctacattt gtagccgtat tgaaaacagg accactggct
     4741 gccatgcaag agcaataccg cccggaagag atagaatcca aagtacagct tcattgggat
     4801 gagaagcgca catttgaagt aaccgaagac gagagcaaag agaagtatta ctgcctgtct
     4861 atgcttccct atccttctgg tcgactacac atgggccacg tacgtaacta caccatcggt
     4921 gacgtgatcg cccgctacca gcgtatgctg ggcaaaaacg tcctgcagcc gatcggctgg
     4981 gacgcgtttg gtctgcctgc ggaaggcgcg gcggtgaaaa acaacaccgc tccggcaccg
     5041 tggacgtacg acaacatcgc gtatatgaaa aaccagctca aaatgctggg ctttggttat
     5101 gactggagcc gcgagctggc aacctgtacg ccggaatact accgttggga acagaaattc
     5161 ttcaccgagc tgtataaaaa aggcctggta tataagaaga cttctgcggt caactggtgc
     5221 ccgaacgacc agaccgtact ggcgaacgaa caagttatcg acggctgctg ctggcgctgc
     5281 gataccaaag ttgaacgtaa agagatcccg cagtggttta tcaaaatcac tgcttacgct
     5341 gacgagctgc tcaacgatct ggataaactg gatcactggc cagacaccgt taaaaccatg
     5401 cagcgtaact ggatcggtcg ttccgaaggc gtggagatca ccttcaacgt taacgactat
     5461 gacaacatgc tgaccgttta cactacccgc ccggacacct ttatgggttg tacctacctg
     5521 gcggtagctg cgggtcatcc gctggcgcag aaagcggcgg aaaataatcc tgaactggcg
     5581 gcctttattg acgaatgccg taacaccaaa gttgccgaag ctgaaatggc gacgatggag
     5641 aaaaaaggcg tcgatactgg ctttaaagct gttcacccat taacgggcga agaaattccc
     5701 gtttgggcag caaacttcgt attgatggag tacggcacgg gcgcagttat ggcggttccg
     5761 ggtcacgacc agcgcgacta cgagtttgcc tctaaatacg gcctgaacat caaaccagtt
     5821 atcctggctg ctgacggctc tgagccagat ctttctcagc aagccctgac tgaaaaaggc
     5881 gtgctgttca actctggcga attcaacggt cttgaccatg aagcggcctt caacgccatc
     5941 gccgataaac tgactgcgat gggcgttggc gagcgtaaag tgaactaccg cctgcgcgac
     6001 tggggtgttt ctcgtcagcg ttactggggc gcgccgattc cgatggtgac gctggaagac
     6061 ggtaccgtaa tgccgacccc ggacgaccag ctgccggtga tcctgccgga agatgtggtg
     6121 atggacggca ttaccagccc gattaaagca gatccggagt gggcaaaaac taccgttaac
     6181 ggtatgccag cgctgcgtga aaccgacact ttcgacacct ttatggagtc ctcctggtac
     6241 tatgcgcgct acacttgccc agagtacaaa gaaggtatgc tggattccga agcggctaac
     6301 tactggctgc cggtggatat ctacattggt ggtatcgaac acgccattat gcacctgctc
     6361 tacttccgct tcttccacaa actgatgcgt gatgcaggca tggtgaactc tgacgaacca
     6421 gcgaaacagt tgctgtgtca gggtatggtg ctggcagatg ccttctacta tgttggcgaa
     6481 aacggcgaac gtaactgggt ttccccggtt gatgctatcg ttgaacgtga cgagaaaggc
     6541 cgtatcgtga aagcgaaaga tgcggcaggc catgaactgg tttataccgg catgagcaaa
     6601 atgtccaagt cgaagaacaa cggtatcgac ccgcaggtga tggttgaacg ttacggcgcg
     6661 gacaccgttc gtctgtttat gatgtttgct tctccggctg atatgactct cgaatggcag
     6721 gaatccggtg tggaaggggc taaccgcttc ctgaaacgtg tctggaaact ggtttacgag
     6781 cacacagcaa aaggtgatgt tgcggcactg aacgttgatg cgctgactga agatcagaaa
     6841 gcgctgcgtc gcgatgtgca taaaacgatc gctaaagtga ccgatgatat cggccgtcgt
     6901 cagaccttca acaccgcaat tgcggcgatt atggagctga tgaacaaact ggcgaaagca
     6961 ccaaccgatg gcgagcagga tcgcgctctg atgcaggaag cgctgctggc cgttgtccgt
     7021 atgcttaacc cgttcacccc gcacatctgc ttcacgctgt ggcaggaact gaaaggcgaa
     7081 ggcgatatcg acaacgcgcc gtggccggtt gctgacgaaa aagcgatggt ggaagactcc
     7141 acgctggtcg tggtgcaggt taacggtaaa gtccgtgcca aaatcaccgt tccggtggac
     7201 gcaacggaag aacaggttcg cgaacgtgct ggccaggaac atctggtagc aaaatatctt
     7261 gatggcgtta ccgtgcgtaa agtgatttac gtaccaggta aactcctcaa tctggtcgtt
     7321 ggctaagcgc gggaggaagc gtgcgatatc tggcaacatt gttgttatct ctggcggtgt
     7381 taatcaccgc cgggtgtggc tggcatctgc gtgataccac gcaggttcct tccactatga
     7441 aggtcatgat cctggactca ggcgatccga acgggccatt aagccgtgcg gtgcgtaacc
     7501 agttacgtct gaatggtgtc gagttgcttg ataaagaaac cacgcgtaag gacgttccat
     7561 ccctgcgttt gggtaaagtg agcatcgcga aagataccgc atcggtattc cgtaacggtc
     7621 aaacagcaga gtatcagatg atcatgacgg tgaatgcgac cgtgttgatc cccggccgtg
     7681 atatctaccc gattagcgcc aaagtcttcc gttcgttctt tgataacccg caaatggcgt
     7741 tagcgaaaga taacgaacaa gacatgatcg taaaagagat gtacgaccgt gctgccgaac
     7801 agctgattcg taagctgcca agcatccgtg ctgcggatat tcgttccgac gaagaacaga
     7861 cgtcgaccac aacggatact ccggcaacgc ctgcacgcgt ctccaccacg ctgggtaact
     7921 gatgattcgg ttgtacccgg aacaactccg cgcgcagctc aatgaagggc tgcgcgcggc
     7981 gtatctttta cttggtaacg atcctctgtt attgcaggaa agccaggacg ctgttcgtca
     8041 ggtagctgcg gcacaaggat tcgaagaaca ccacactttt tccattgatc ccaacactga
     8101 ctggaatgcg atcttttcgt tatgccaggc tatgagtctg tttgccagtc gacaaacgct
     8161 attgctgttg ttaccagaaa acggaccgaa tgcggcgatc aatgaacaac ttctcacact
     8221 caccggactt ctgcatgacg acctgctgtt gatcgtccgc ggtaataaat taagcaaagc
     8281 gcaagaaaat gccgcctggt ttactgctct tgcgaatcgc agcgtgcagg tgacctgtca
     8341 gacaccggag caggctcagc ttccccgctg ggttgctgcg cgcgcaaaac agctcaactt
     8401 agaactggat gacgcggcaa atcaggtgct ctgctactgt tatgaaggta acctgctggc
     8461 gctggctcag gcactggagc gtttatcgct gctctggcca gacggcaaat tgacattacc
     8521 gcgcgttgaa caggcggtga atgatgccgc gcatttcacc ccttttcatt gggttgatgc
     8581 tttgttgatg ggaaaaagta agcgcgcatt gcatattctt cagcaactgc gtctggaagg
     8641 cagcgaaccg gttattttgt tgcgcacatt acaacgtgaa ctgttgttac tggttaacct
     8701 gaaacgccag tctgcccata cgccactgcg tgcgttgttt gataagcatc gggtatggca
     8761 gaaccgccgg ggcatgatgg gcgaggcgtt aaatcgctta agtcagacgc agttacgtca
     8821 ggccgtgcaa ctcctgacac gaacggaact caccctcaaa caagattacg gtcagtcagt
     8881 gtgggcagag ctgaaagggt tatctcttct gttgtgccat aaacccctgg cggacgtatt
     8941 tatcgacggt tgatatgaaa tctttacagg ctctgtttgg cggcaccttt gatccggtgc
     9001 actatggtca tctaaaaccc gtggaaacgc tggcgaattt gattggtctg acgcgggtca
     9061 caatcatccc taataatgtt cctccgcatc gtccccagcc ggaagcgaac agcgtgcagc
     9121 gtaaacacat gcttgaactg gcgattgccg acaagccatt atttactctt gatgaacgcg
     9181 agctaaagcg caatgccccc tcttacactg cgcaaacact gaaagagtgg cggcaggaac
     9241 aaggaccgga cgtgccgctg gcgtttatta ttggtcagga ttcactgctg acctttccga
     9301 cctggtacga atacgaaacg atactcgaca atgcacattt gatcgtctgt cggcgtccag
     9361 gttacccact tgaaatggcg caaccgcaat accagcaatg gctggaagat catttgacac
     9421 ataacccgga agatcttcac cttcagcctg ccggtaaaat ttatctggct gaaacgccgt
     9481 ggtttaacat ctcggcgacc atcatccgcg aacgtttgca aaacggtgaa tcgtgtgagg
     9541 atttattgcc ggaaccggta ctgacttaca ttaaccaaca aggcttgtat cgctgatacc
     9601 tgtcgttacg gagggcatga tgcgactgtg gttaattcgt catggtgaaa cgcaagcgaa
     9661 tatcgatggt ctttacagcg gtcatgcgcc cacccccctg accgcgcgcg gtattgagca
     9721 agcgcaaaat ctgcatacgc tgctacatgg tgtttccttt gatctggttt tatgcagtga
     9781 actggaacgg gcacagcata ccgcgcgact ggttctcagt gaccgccagc tccccgtgca
     9841 aatcatccct gaactcaacg aaatgttttt tggcgactgg gagatgcgac atcatcgcga
     9901 cctcatgcaa gaagatgccg aaaactatag cgcgtggtgc aatgactggc agcatgcaat
     9961 ccccacgaac ggtgaaggat ttcaggcatt ttcgcaacgt gtggaacgct ttatcgcaag
    10021 gcttagtgaa tttcagcact atcagaatat tttagtcgtc agccatcagg gtgtactgag
    10081 tctgttaatc gcccgtttaa ttggcatgcc tgccgaggct atgtggcatt ttcgcgttga
    10141 ccaggggtgt tggagcgcca ttgatatcaa ccaaaaattc gcgacgctac gcgtcctcaa
    10201 tagccgtgcc atcggggtcg agaatgcatg acttttctgt ttttttacgg gtaagccgca
    10261 acgaccattg acagacccgg gcaggctgat attctccgca gccagacttt tttcgccaga
    10321 cacgactttg tagaaattgt tttacaaaaa tggcgatgca atctgcggcg cggggtggga
    10381 tgatagccca ctttcgaaag ccgattcggc gacaattgtc ccgaaatcgc ctctggttca
    10441 ggtatactga cagaccattt ttatctattt gattcaccca gggggaaaac ttgcagggta
    10501 aagcactcca ggattttgtt atcgacaaaa ttgatgacct caaaggtcag gacatcatcg
    10561 ccttagacgt tcaaggcaaa tccagcatca ccgactgcat gatcatctgt acgggtacgt
    10621 ccagccgtca tgttatgtcc attgctgacc acgttgtgca ggagtctcgc gcagcgggcc
    10681 tgttaccgct cggcgtagaa ggtgaaaaca gcgccgactg gattgtcgtg gatttgggcg
    10741 atgtgattgt ccatgtcatg caggaagaga gccgtcgcct gtatgaactg gaaaaactct
    10801 ggagttaatg cgtgaagctg caacttgtcg ccgtgggaac gaaaatgccg gactgggtac
    10861 aaaccggttt taccgagtac ctgcgtcgtt ttccgaaaga tatgcccttc gagctgattg
    10921 aaattccggc cggaaaacgc ggcaagaatg cggacatcaa gcgcatactc gacaaagagg
    10981 gtgagcagat gttggcggcc gcaggcaaaa accgcattgt caccctcgat attccaggca
    11041 agccctggga tacgccgcag ttagccgctg agctggaacg ctggaagctg gatggtcgcg
    11101 acgtcagtct actgattggc gggcctgaag ggttgtcgcc tgcctgtaaa gcggcggcgg
    11161 agcagagctg gtcgctgtcg gcgcttaccc tcccccatcc gctggttcgc gtgctggtcg
    11221 cagagagtct gtaccgggcg tggagcatca ccaccaacca tccttatcac cgtgagtgat
    11281 aagggagctt tgagtagaaa acgcagcgga tgaaactaca gaactctttt cgcgactata
    11341 cggcagagtc cgcgctgttt gtgcgccggg cgctggtcgc ctttttgggg attttgctgc
    11401 tgaccggcgt gcttatcgcc aacctgtata atctgcaaat tgttcgcttt accgactacc
    11461 agactcgctc taatgaaaac cgcattaagc tggtgcctat cgcgcccagc cgcggcatta
    11521 tctacgaccg taatggtatc cctctggccc tcaaccgcac tatctaccag atagaaatga
    11581 tgccagagaa agtcgataac gtgcagcaaa cgctggacgc tttgcgcagc gtggttgatc
    11641 tgaccgatga cgatattgct gcattccgaa aagagcgcgc acgttcacac cgtttcacct
    11701 ctattccggt gaaaactaac ctgaccgaag tacaagtagc tcgctttgcc gtcaatcagt
    11761 accgttttcc gggtgtcgaa gttaaaggct ataaacgtcg ttactatcct tacggttcgg
    11821 cgttgaccca cgtcatcggc tatgtgtcga aaatcaacga taaagacgtc gaacgcctga
    11881 ataatgacgg caaactggcc aactatgcgg caacgcatga tatcggtaag ctgggcattg
    11941 agcgttacta tgaagacgtg ctgcacggtc agaccggtta tgaagaggtt gaagttaaca
    12001 accgtgggcg tgttatccgc cagttaaaag aagtaccacc gcaagccgga cacgatattt
    12061 acctgacgct ggatctcaaa ctccagcaat atattgaaac gctgctggcg ggtagccgcg
    12121 cagctgtggt tgtcaccgat ccgcgtacag gtggggtgct ggcgctggtt tccacgccta
    12181 gttatgaccc aaacctgttt gttgacggta tctccagcaa agattattcc gccttgttga
    12241 acgccccgaa tacaccgctg gtgaaccgcg ccacacaggg ggtttatcct cccgcgtcta
    12301 cagttaaacc ctatgtggcg gtttcggcat tgagcgccgg ggtgatcacg cgcaatacga
    12361 cgctgtttga cccaggctgg tggcaactgc caggttcgga aaaacgttat cgtgactgga
    12421 aaaaatgggg ccacgggcgt ctgaatgtca caagatcgct ggaagaatct gcggatacct
    12481 tcttctatca ggtggcctac gatatgggga tcgatcgcct ctccgaatgg atgggtaaat
    12541 tcggttatgg tcattacacc ggtatcgacc tggcggaaga acgttccggc aacatgccta
    12601 cccgcgaatg gaaacagaaa cgctttaaaa aaccgtggta tcagggtgac accattccgg
    12661 ttggtatcgg tcagggttac tggacagcga ccccaatcca gatgagtaag gcactgatga
    12721 tcctgattaa tgacggtatc gtgaaggttc ctcatttgct gatgagcacc gccgaagacg
    12781 gcaaacaggt gccatgggta cagccgcatg aaccgcccgt cggcgatatt cattccggtt
    12841 actgggagct ggcgaaagac ggtatgtacg gtgttgctaa ccgccctaac ggtacggcgc
    12901 ataaatactt tgccagcgca ccgtacaaaa ttgcggcgaa atccggtacc gctcaggtct
    12961 tcggtctgaa agcgaacgaa acctataatg cgcacaaaat tgccgagcgt ttacgtgacc
    13021 acaaactgat gaccgccttt gcgccataca acaatccgca agtggctgtc gccatgattc
    13081 tggagaacgg tggtgcgggt ccggcggttg gtacactgat gcgccagatc ctcgaccaca
    13141 ttatgctggg tgataacaac accgatctgc ctgcggaaaa tccagcggtt gccgcagcgg
    13201 aggaccatta atcatgacgg ataatccgaa taaaaaaaca ttctgggata aagtccatct
    13261 cgatcccaca atgctgctga tcttactggc attgctggtt tacagcgccc tggttatctg
    13321 gagcgccagc ggtcaggata ttggcatgat ggagcgtaaa atcggccaaa tcgcgatggg
    13381 tctggtcatc atggtggtga tggcgcaaat tcctccacgc gtttatgaag gctgggcccc
    13441 ctatctctat atcatctgta ttattttgct ggtggcggta gatgctttcg gtgccatctc
    13501 taaaggtgct caacgctggc tggacctcgg tattgttcgt tttcagccgt cggaaattgc
    13561 caaaatagcc gtaccactga tggttgcgcg ctttatcaac cgcgacgttt gcccgccatc
    13621 gttgaagaac actggcatcg cgctggtgct gatatttatg cccacgctgc tggtggctgc
    13681 acagcctgac ctgggaacat caatcctcgt tgcgctttcc ggtctgtttg tactgttcct
    13741 ctctggcctt agctggcgtc tgattggcgt cgcagtagtg ctggtggcgg cgttcattcc
    13801 gattctgtgg ttcttcctga tgcatgatta ccagcgccag cgcgtaatga tgctcctaga
    13861 cccggaatca gacccactcg gcgcgggcta tcacattatt cagtctaaaa ttgctattgg
    13921 ctccggcgga ttacgcggca aaggctggct gcacggcact cagtcacagc ttgaatttct
    13981 ccccgaacgc cataccgact ttatcttcgc ggtactggcg gaagagctgg gattagtggg
    14041 cattctgatt ctgctcgctc tctacattct gctgatcatg cgcgggctgt ggatagccgc
    14101 cagagcgcaa accacctttg gtcgcgtcat ggctggtggc ttaatgctga tattattcgt
    14161 ttatgtcttc gtaaatattg gtatggtaag cggtattctg ccggttgtag gggttccgct
    14221 cccactggtc agttatggag gatcggcgct aattgtgctg atggctgggt tcgggattgt
    14281 aatgtcaatc cacacccaca ggaaaatgtt gtcgaaaagc gtgtaagagg tgcgcaatgc
    14341 gtaagcagtg gctcgggatc tgcatcgcgg caggaatgct cgcggcatgt acaagcgatg
    14401 atggtcagca acagacggta agtgtaccgc agcctgcggt atgtaacggc cccatagttg
    14461 aaattagcgg ggcggacccg cgtttcgaac cactgaacgc gacggcaaat caggattacc
    14521 agcgcgacgg taaaagctac aaaatcgtgc aggatccgtc tcgatttagc caggcgggac
    14581 tggcggcaat ctatgatgcc gaacccggca gtaacctgac ggcctctggc gaagctttcg
    14641 atccgacaca gctgacggcg gcccatccaa cgcttccgat ccccagctac gccagaatca
    14701 ctaacctggc taacgggcga atgatcgtgg tgcgcattaa tgatcgcggc ccttacggca
    14761 acgaccgcgt tatttcactt tctcgcgcgg cagctgaccg tcttaacacg tcaaacaaca
    14821 ccaaagttcg tatcgatccg attattgtcg cccaggatgg ttcgctttct ggtcctggta
    14881 tggcttgtac cacagtcgcc aaacagactt acgccctgcc agcacctccc gatttaagcg
    14941 gtggcgcggg aacaagttca gtgtctggcc cgcagggtga cattcttccg gtcagtaatt
    15001 cgacgctaaa aagcgaagat ccgaccggcg cgccggtaac cagtagcggt ttcctcggcg
    15061 ctccaacgac cttagcgcct ggtgtactgg aaggcagcga accgacgcct gcgccacagc
    15121 ccgttgttac agctccgtcg acaacgcctg caacctcgcc tgcaatggtg acaccgcaag
    15181 ccgcctcgca aagcgccagc ggcaacttta tggtgcaagt cggggccgta agcgatcagg
    15241 ctcgtgcgca acagtaccaa cagcaactgg gacagaagtt cggcgtcccc ggtcgcgtaa
    15301 ctcaaaatgg cgcggtctgg cggatccagc ttggcccatt cgccagcaaa gccgaagcca
    15361 gtaccttgca gcaacgtttg caaaccgaag cccaattaca gtcatttatt actaccgcgc
    15421 agtagcgtaa agcaggcatc tgatgtgtca atttctgtaa atgacgttaa caaagtcacg
    15481 cggaaagtca gatgcctgcc ggtagtgcat ttgctatagt agggcacttt tttaattcca
    15541 tcacggatgt cgtagttcag accatgaata ccattttttc cgctcgtatc atgaagcgcc
    15601 tggcgctcac cacggctctt tgcacagcct ttatctctgc tgcacatgcc gatgacctga
    15661 atatcaaaac tatgatcccg ggtgtaccgc agatcgatgc ggagtcctac atcctgattg
    15721 actataactc cggcaaagtg ctcgccgaac agaacgcaga tgtccgccgc gatcctgcca
    15781 gcctgaccaa aatgatgacc agttacgtta tcggccaggc aatgaaagcc ggtaaattta
    15841 aagaaactga tttagtcact atcggcaacg acgcatgggc caccggtaac ccggtgttta
    15901 aaggttcttc gctgatgttc ctcaaaccgg gcatgcaggt tccggtttct cagctgatcc
    15961 gcggtattaa cctgcaatcg ggtaacgatg cttgtgtcgc catggccgat tttgccgctg
    16021 gtagccagga cgcttttgtt ggcttgatga acagctacgt taacgcactg ggcctgaaaa
    16081 atacccactt ccagacggta catggtctgg atgctgatgg tcagtacagc tccgcgcgag
    16141 atatggcgct gatcggccag gcattgatcc gtgacgtacc gaatgaatac tcgatctata
    16201 aagaaaaaga atttacgttt aacggtattc gccagctgaa ccgtaacggc ctgttatggg
    16261 ataacagcct gaatgtcgac ggcatcaaaa ccggacacac tgacaaagca ggttacaacc
    16321 ttgttgcttc tgcgactgaa ggccagatgc gcttgatttc tgcggtgatg ggcggacgta
    16381 cttttaaagg ccgtgaagcc gaaagtaaaa aactgctaac ctggggcttc cgtttcttcg
    16441 aaaccgttaa cccactgaaa gtaggtaaag agttcgcctc tgaaccggtt tggtttggtg
    16501 attctgatcg cgcttcgtta ggggttgata aagacgtgta cctgaccatt ccacgtggtc
    16561 gcatgaaaga tctgaaagcc agctatgtgc tgaacagcag tgaattgcat gcgccgctgc
    16621 aaaagaatca ggtcgtcgga actatcaact tccagcttga tggcaaaacg atcgagcaac
    16681 gcccgctggt tgtgttgcaa gaaatcccgg aaggtaactt cttcggcaaa atcattgatt
    16741 acattaaatt aatgttccat cactggtttg gttaaaaatt gaacacttga aagtgtaatt
    16801 tccgtcccca tatactaagc atcagtaaaa aactcccgcc ttctggcggg agttgctatt
    16861 taattacgtt acgccggagc tgacatgaaa accaaactta acgaactgct tgaattccct
    16921 actcctttta cttacaaagt tatggggcag gcgttacctg agctggttga tcaggtggtt
    16981 gaagtggtac agcgccatgc gccaggtgac tacaccccaa cggtaaaacc aagcagcaaa
    17041 ggcaactacc actcggtatc tatcactatc aacgccactc atatcgagca ggttgaaaca
    17101 ctgtatgaag aactaggcaa aatcgatatt gtccgcatgg ttctgtaact cgcttctccg
    17161 ttacccggcc cctggtcggg taactcccct ctcctcgctg tgatatactt ttcccccact
    17221 tttactcatt ctccacggag atgccgtttt gtatcaggat aaaattcttg tccgccagct
    17281 cggtcttcag ccttacgagc caatctccca ggctatgcat gaattcaccg atacccgcga
    17341 tgatagtacc cttgatgaaa tctggctggt cgagcactat ccggtattca cccaaggtca
    17401 ggcaggaaaa gcggagcaca ttttaatgcc gggtgatatt ccggtgatcc agagcgatcg
    17461 cggtgggcag gtgacttatc acgggccggg gcaacaggtg atgtatgtgt tgcttaacct
    17521 gaaacgccgt aaactcggtg tgcgtgaact ggtgaccttg cttgagcaaa cagtggtgaa
    17581 taccctggct gaactgggta tagaagcgca tcctcgggct gacgcgcctg gtgtctatgt
    17641 cggggaaaag aaaatttgct cactgggttt acgtattcga cgcggttgtt cattccacgg
    17701 tctggcatta aacgtcaata tggatctttc accattttta cgtattaatc cttgtgggta
    17761 tgccggaatg gaaatggcta aaatatcaca atggaaaccc gaagcgacga ctaataatat
    17821 tgcaccacgt ttactggaaa atattttagc gctactaaac aatcctgact tcgaatatat
    17881 taccgcttaa ttgcatacct caatggccca atttacactg ggtcattact cataatacct
    17941 gctaatgctt tacatctcct tcgcgtttgt atactttctt tatcacttgc agaaatgcgg
    18001 tgctacgcct tgctccgtaa ctcaccccat tgattaacct attgaaatag ttgattatgg
    18061 tgagcaaaag cgttccgttc caattagaca taagattatt gtaatttttt taaatattat
    18121 ttttccatga ataaaattgg agtaagtgcg tggatagtaa taatcaaatt gaaccctgtt
    18181 taagcagaaa gtcgtcagaa ggtaaaccac aaatatttac aaccttacga aatattgatc
    18241 ttaaccttct gactattttt gaagctgtat atgtacataa agggatcgtt aatgcagcga
    18301 aagtgcttaa tctgaccccc tcggcaatca gtcagtctat tcagaaactg cgcgttatat
    18361 tccctgaccc attgtttatt cgcaaaggcc agggtgtcac tcctaccgca tttgcgatgc
    18421 atctacatga gtatatcagt cagggccttg agtccattct tggcgcgctg gatatcgaag
    18481 gaagctatga taagcaacga acgataacta ttgcgaccac tccctcggtc ggagccctgg
    18541 tccttcctgt catctatcgg gcgattaaaa ctcactatcc gcagctttta ctgcgcaacc
    18601 cgcccatcag cgacgcggaa aaccaactca gtcagtttca aaccgatctc atcatcgata
    18661 acatgttttg caccaatcgt acggtgcaac atcatgttct gttcaccgac aatatggtgt
    18721 taatttgccg cgagggaaat ccactactct ctttagaaga tgacagagag actatcgaca
    18781 acgctgcgca tgtactcctg ttaccggaag ggcaaaattt cagcggtctg cggcagagag
    18841 ttcaagagat gtttccggac cggcaaatca atttcaccag ctacaacatt ttgacaatcg
    18901 ctgcactggt tgccaacagt gacatgttag cgattattcc cagccgtttt tataacctgt
    18961 ttagtcgttg ctggccgctg gaaaaactgc cttttccgtc cttaaatgag gagcaaatag
    19021 atttctctat ccactacaac aaattcagcc tgcgtgatcc catcctgcac ggggtaatag
    19081 atgttatccg taatgcattt tgaaaaagta ttgttacagc cagatcacag cgcagaacga
    19141 taaaatggca caatccaaca acaattttac attttcgcga ccgctttggc tgctttcagg
    19201 tccgtttcaa tgatatactg ccagtcgtta attcaaaaat agttgataat tacaacaatc
    19261 tattgaattg aaacgctttc cttcgtaatt cgcaactgga acacgcacgc tatgagtaaa
    19321 cccattgtga tggaacgcgg tgttaaatac cgcgatgccg ataagatggc ccttatcccg
    19381 gttaaaaacg tggcaacaga gcgcgaagcc ctgctgcgca agccggaatg gatgaaaatc
    19441 aagcttccag cggactctac acgtatccag ggcatcaaag ccgcaatgcg caaaaatggc
    19501 ctgcattctg tctgcgagga agcctcctgc cctaacctgg cggaatgctt caaccacggc
    19561 acagcaacgt ttatgatcct cggcgctatt tgtacccgcc gttgtccgtt ctgtgatgtt
    19621 gcccacggtc gcccggtagc tcctgatgcc aatgaaccag tgaaactggc gcagaccatt
    19681 gccgatatgg cgctgcgtta tgtggttatc acctccgttg accgtgatga cctgcgcgat
    19741 ggcggtgccc agcactttgc ggattgcatt actgccattc gggaaaaaag cccgcaaatc
    19801 aaaattgaaa ctctggtgcc ggatttccgt ggtcgtatgg atcgtgctct ggatattctg
    19861 actgcaacgc caccagatgt gttcaaccat aacctggaaa acgtaccgcg tatttaccgt
    19921 caggtacggc ctggtgcaga ttacaactgg tcgctgaagc tgctggaacg ctttaaagaa
    19981 gcgcatccgg aaatcccgac caagtctggt ctgatggtgg gactgggtga aaccaatgaa
    20041 gaaattattg aggtaatgcg cgacctgcgc cgtcatggtg tgacgatgtt aacgctgggg
    20101 caatatttgc agccaagccg ccatcacctg ccggttcaac gttacgttag cccggatgag
    20161 ttcgacgaaa tgaaagccga agcgctggcg atgggcttta cccatgctgc atgcggtccg
    20221 tttgtccgct cttcttacca cgccgatttg caggcgaaag ggatggaagt taagtaatcc
    20281 tgtaataatt acttacatct gtctgataaa aaacccgctt tttg
//
LOCUS       DAKISA010000083        20241 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.083, whole genome shotgun sequence.
ACCESSION   DAKISA010000083 DAKISA010000000
VERSION     DAKISA010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20241
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.083"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            690..1460
                     /locus_tag="OVW45_003002"
     CDS             690..1460
                     /locus_tag="OVW45_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCV5527395.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1451..1924
                     /locus_tag="OVW45_003003"
     CDS             1451..1924
                     /locus_tag="OVW45_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCV5527396.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            2032..2571
                     /gene="dnaT"
                     /locus_tag="OVW45_003004"
     CDS             2032..2571
                     /gene="dnaT"
                     /locus_tag="OVW45_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCV5527397.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2574..3311
                     /gene="dnaC"
                     /locus_tag="OVW45_003005"
     CDS             2574..3311
                     /gene="dnaC"
                     /locus_tag="OVW45_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCV5527398.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3360..3854
                     /locus_tag="OVW45_003006"
     CDS             3360..3854
                     /locus_tag="OVW45_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCV5527399.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            4108..6399
                     /gene="opgB"
                     /locus_tag="OVW45_003007"
     CDS             4108..6399
                     /gene="opgB"
                     /locus_tag="OVW45_003007"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCV5527400.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6538..7560)
                     /gene="lgoD"
                     /locus_tag="OVW45_003008"
     CDS             complement(6538..7560)
                     /gene="lgoD"
                     /locus_tag="OVW45_003008"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HCV5527401.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7699..8613
                     /locus_tag="OVW45_003009"
     CDS             7699..8613
                     /locus_tag="OVW45_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCV5527402.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFGQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIHSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8828..10189
                     /locus_tag="OVW45_003010"
     CDS             8828..10189
                     /locus_tag="OVW45_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCV5527403.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10238..11893)
                     /gene="tsr"
                     /locus_tag="OVW45_003011"
     CDS             complement(10238..11893)
                     /gene="tsr"
                     /locus_tag="OVW45_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCV5527404.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQRETSAVVKTVTPAAPRKMAVADSEENWETF"
     gene            complement(12012..12458)
                     /gene="hpaR"
                     /locus_tag="OVW45_003012"
     CDS             complement(12012..12458)
                     /gene="hpaR"
                     /locus_tag="OVW45_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCV5527405.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
     gene            12730..14019
                     /locus_tag="OVW45_003013"
     CDS             12730..14019
                     /locus_tag="OVW45_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCV5527406.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTHKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            14016..15482
                     /gene="hpaE"
                     /locus_tag="OVW45_003014"
     CDS             14016..15482
                     /gene="hpaE"
                     /locus_tag="OVW45_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCV5527407.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADSLGELALEADIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15484..16335
                     /gene="hpaD"
                     /locus_tag="OVW45_003015"
     CDS             15484..16335
                     /gene="hpaD"
                     /locus_tag="OVW45_003015"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCV5527408.1"
                     /translation="MGKLALAAKITHVPSMYHSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16345..16725
                     /locus_tag="OVW45_003016"
     CDS             16345..16725
                     /locus_tag="OVW45_003016"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCV5527409.1"
                     /translation="MPHFIVKCSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            16836..17639
                     /gene="hpaH"
                     /locus_tag="OVW45_003017"
     CDS             16836..17639
                     /gene="hpaH"
                     /locus_tag="OVW45_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCV5527410.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17650..18438
                     /gene="hpaI"
                     /locus_tag="OVW45_003018"
     CDS             17650..18438
                     /gene="hpaI"
                     /locus_tag="OVW45_003018"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCV5527411.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HTPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            18613..19989
                     /gene="hpaX"
                     /locus_tag="OVW45_003019"
     CDS             18613..19989
                     /gene="hpaX"
                     /locus_tag="OVW45_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCV5527412.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLSVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGMLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATL"
     gene            19999..>20241
                     /locus_tag="OVW45_003020"
     CDS             19999..>20241
                     /locus_tag="OVW45_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCV5527413.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPS"
BASE COUNT         4869 a         5432 c         5173 g         4767 t
ORIGIN      
        1 tcttttaacc ctgcttgcat aacaggtatt ttactgataa caattccatt tttgcagcat
       61 cctggcaaca tatttcctcc acatccactg tctaaaaatc atgatggttt attcaggata
      121 ttcattcctt aaatgtttcc agcaaattaa tagattaagc gaataaatta tccttagcat
      181 tcactttcat atcagcatac tcctgctgca caacaactcc actgcatgtg tcagaaggtt
      241 agcatcaaat gataacctgg tgaaatagcc tggagacatt taaaatatct acattaagtt
      301 gttaggaata cttaatgaaa aacagaaatc acattcattt aatttataat taaatgatat
      361 cccttcccgc agaatgaaaa atcacacaac caatatttat tcaatgaaat ctgatggttt
      421 taacttgtta acattaaaaa acgtgcccgg ttatctatta tcctgattcg taaaggccaa
      481 acaataaatt cattatagtg ataatattca tgtgatagtt tgtttgcggc gagagataat
      541 tcgcttttta tcaccgagct tgtcccgata accaggatta atcagcgcaa ttttgatctt
      601 aacctaccgc aaaacaaccg tgcttcatga tacaaatgga ttctcattat ctgaacaata
      661 cgcagcacgt ctatgacaaa gggcgagtta tgcaaactga gcaacagcga gccgtaacac
      721 ggctttgtat ccagtgtgga ttatttcttt tgcaacatgg tgcggaaagc gcgttggttg
      781 atgagctttc ctcacgactg ggtcgggcgt tggggatgga cagcgtcgaa agttctatct
      841 cttcgaacgc catagtgctg acaaccatta aagatgggca atgcctgaca tcgacacgta
      901 aaaatcacga tcgcggcatt aatatgcatg tggtgactga agtccagcac attgtgattc
      961 tggcggagca ccatctgctg gattacaaag gcgtagagaa acgatttagc caaattcagc
     1021 cattacgtta cccaagatgg ctggtagcct taatggttgg cctttcttgc gcctgtttct
     1081 gcaaactcaa taaaggtggc tgggatggtg ccgtcatcac cttctttgcc agtacggccg
     1141 cgatgtatat ccgccagctg ctggcacaac gtcatcttca tccgcagatc aacttttgcc
     1201 tcaccgcttt cgccgccacc accatttccg gattgctttt gcaacttccc actttcagca
     1261 atacacccac tatcgcgatg gccgccagcg ttctgctgct ggtgccggga tttccgttga
     1321 ttaatgccgt cgccgatatg tttaaaggcc acatcaatac cggactggca cgctgggcga
     1381 tcgccagtct gctgacactg gctacctgcg tcggcgtggt gatggcactg acgatttggg
     1441 ggctacgcgg atgggtgtga tcgagtttct gtttgcattg gcgcaggata tgatcctcgc
     1501 cgccattcca gcggtcggct ttgcgatggt gttcaacgtt cccgttcggg cattacgctg
     1561 gtgtgcgctg cttggcgcga ttggtcatgg ttcacgaatg atcttaatga ccagcgggct
     1621 gaatattgag tggtcaacct ttatggcctc catgctggtc ggcaccattg gtattcaatg
     1681 gtcgcgctgg tatctggcgc atccgaaagt gtttaccgtg gcggccgtta tccctatgtt
     1741 cccgggcata tcggcttata ccgcaatgat ttcggcggta aaaatcagcc agttaggtta
     1801 cagcgaaccg ttgatgatta ccctgttaac caactttctt acagcttcat cgattgttgg
     1861 tgcgttatcc atcggtcttt ccattcctgg attatggttg taccgcaagc gccctcgcgt
     1921 ataaaattgc ctcgcttaaa gcacacggat gagagacagc ctcctctcct ccgtgtgtta
     1981 ctataaaagt tatctccctt ctcgttcatc gttccatatt tgagaaacag tatgtcttcc
     2041 agagttttga ccccggacgt cgttggtatt gacgccctgg tacacgatca ccaaaccgtt
     2101 ctggcaaaag ctgaaggcgg tgtggttgcc gtatttgcta acaatgcccc ggcgttttat
     2161 gccgtcacgc ctgcacgcct ggctgaactg ctggcgctgg aagaaaagct ggcgcgtccg
     2221 ggaagcgatg tcgctctgga cgatcaactc tatcaggaac cgcaagccgc tcccgttgct
     2281 gtacccatgg ggaaattcgc catgtatccg gactggcaac ccgatgccga ttttatccgc
     2341 ctggcggcgc tatggggtgt ggcactaaga gagccggtga ccaccgaaga actggcctca
     2401 ttcattgcct actggcaggc ggaaggtaaa gtctttcacc atgtgcagtg gcaacaaaaa
     2461 ctggcgcgca gcctgcaaat cggtcgtgcc agcaacggcg gactgccgaa acgagatgtg
     2521 aatacggtca gcgaacctga cagtcaaatt ccaccaggat tcagagggta acgatgaaaa
     2581 acgttggcga cctgatgcaa cgcctgcaaa aaatgatgcc tgcccatatc aaacctgcat
     2641 tcaaaacggg tgaagaacta ctggcctggc agaaagaaca aggagcgatc cgctccgccg
     2701 ctctcgaacg tgaaaatcgg gcgatgaaaa tgcagcgcac ctttaaccgc tccggtattc
     2761 ggccactgca tcagaactgc tcctttgaga actatcgcgt tgagtgtgaa gggcagatga
     2821 atgcgttaag caaagcgcgc cagtacgtcg aagagtttga cggcaacatc gccagcttca
     2881 tcttttctgg taagccagga accggcaaaa accatctggc ggcggcaatc tgcaacgagc
     2941 tgctgctacg cggtaaatcc gtattgatca tcaccgtggc cgatattatg tcggcgatga
     3001 aagatacctt caggaatagc ggtaccagtg aagaacaact gcttaacgat ctgagcaacg
     3061 ttgatctgct ggtgatcgat gagatcggcg tgcagaccga atcgaaatac gaaaaagtga
     3121 tcatcaacca gatcgtcgat cgccgctctt cttccaaacg cccaaccggg atgctgacca
     3181 acagtaatat ggaagagatg accaagttgc tgggcgaacg cgtgatggac cgtatgcgcc
     3241 tgggtaacag tttgtgggtg atcttcaact gggatagcta ccgtagccgg gtaacaggta
     3301 aagagtatta atccccgttt ctggcggtat actgcgacac aattccacct tgagtaatga
     3361 tgatgaaaac tgttaaacat cttctgtgct gtgccattgc cgccagcgca ttaatttcca
     3421 ccggggtgca tgctgcgtcc tggaaagatg cgctctccag cgccgccagc gaacttggca
     3481 accaaaacag cacgacacag gaaggcggtt ggtcgctcgc gtcattaact aacttgctta
     3541 gcagcgggaa ccaggcctta agcgcagata acatgaacaa cgccgcaggc attctgcaat
     3601 actgcgcgaa gcaaaagctg gcgtcggtaa ccgatgccga aaacatcaag aaccaggtgc
     3661 tggaaaagct gggcctgaac agtgaagagc aaaaagaaga caccaactat ctggatggca
     3721 ttcagggttt gctgaaaacc aaagatggtc agcaactcaa tctggataac atcggaacga
     3781 ctccgctggc agaaaaggtg aaaaccaaag cctgcgatct ggtgttaaag caggggctga
     3841 acttcatttc ctgaaccgcg aaacgccgcg ttttagctgt tacccggctg acgcggctct
     3901 ctcctgccct tttccctgct cctggacggt tttccccctg ttacacggga agcgaccgcg
     3961 atcaaaaaag tctgtttata aactaattcc taacaacatc gcattttttt gacactagaa
     4021 ttgcagcaat atggcgacgc cattacattg tgcttctcaa aaagcttaaa cgttggctaa
     4081 acggcccggt ttagtgagga tcgcccgttg tcagaactac tctctttcgc cctttttctc
     4141 gcctctgtgc tgatttacgc atggaaagcg ggacgtaaca cctggtggtt tgcagccaca
     4201 ttaacggtgc tggggctatt tgtcgtttta aatatcaccc tgtttgccag cgactatttt
     4261 actggcgatg gtattaacga cgcggttctc tataccttaa ccaacagcct gaccggtgct
     4321 ggcgtcagca aatacatcct gccgggtatc ggcattgtgc tggggctgac agcggtgttc
     4381 ggtgcgctgg gctggatcct gcgccgtcgt cgccatcatc cgcaccattt tggttacagc
     4441 ctgctggcgc tcttactggc gctgggttcg gtggacgcca gcccggcatt tcgtcagata
     4501 acggaactgg tgaaatccca gtcacgcgat ggcgacccgg actttgcggc ttattataaa
     4561 gagccgtcga aaactatccc tgacccgaaa ctcaacctgg tttatatcta cggcgaaagt
     4621 ctcgagcgga cctattttga taacgaagct ttcccggatc tcacgcctga actgggcgcg
     4681 ttgaaaaatg aaggcctgga tttcagccac acgcagcagc tgccaggaac ggattacacg
     4741 attgcgggca tggtggcttc tcagtgcggc ataccgctgt ttgccccctt tgaaggcaac
     4801 gcctccgcct ctgtctccag cttcttcccg cagaacatct gtctgggcga tatcctgaaa
     4861 aactcgggtt atcagaacta tttcgtgcag ggcgcgaatc tgcgttttgc cggtaaagat
     4921 gtgttcctga agtcgcatgg cttcgaccac ttatacggct cagaagagct gaaaagcgtg
     4981 gtggccgacc cgcactatcg caacgactgg ggattctacg acgataccgt tctcgatgaa
     5041 gcgtggaaaa agtttgaaga gctttcccgc tcaggtcagc gattctcact gtttaccctg
     5101 acagtcgata cccatcaccc ggatggtttt atctcgcgta cctgtaaccg caaaaaatat
     5161 gattttgacg gtaagccgaa ccagtcattc agcgcggtaa gttgcagcca ggagaatatc
     5221 gcggcgttta tcaacaaaat caaagcgtca ccgtggttta aagataccgt catcgtcgtc
     5281 tcttctgacc atttagcgat gaacaacacg gcgtggaaat acctcaataa gcaggatcgc
     5341 aataacctgt tttttgtcat tcgtggcgac aagccgcagc aagagacgct ggcagtgaag
     5401 cgtaacacga tggataacgg cgcgacggtg ctggacattc tcggtggcga taactatctc
     5461 ggacttggtc gtagcagttt atccgggcag tcgatgtcgg aaatcttcct caatatcaaa
     5521 gagaaaacat tggcgtggaa gccggatatc atccgcctgt ggaaattccc taaagagatg
     5581 aaagagttca ccatcgacca gcagaaaaac atgattgcct tctcgggtag ccatttccgt
     5641 ttgccgctgc tgttgcgggt ttcagacaaa cgcgtggaac cgctgccgga aagcgaatac
     5701 tcagcaccgc tgcgtttcca gctggccgat ttcgctccac gcgacaattt cgtctgggtt
     5761 gaccgttgct ataagatggc acaactctgg gctccggaac tggcactctc caccgactgg
     5821 tgtgtctcgc aagggcagct tggcggtcag caaattgttc agcatgttga caaaacaaca
     5881 tggcagggca aaacggcatt taaagatacg gtcatcgaca tggcgcgtta taaaggcaat
     5941 gtcgatacgc tgaaaattgt tgataacgat attcgctaca aagccgacag tttcatcttc
     6001 aacgtcgccg gtgcgccaga agaggtgaaa cagtttagtg ggatttcccg tccagagtcg
     6061 tggggccgct ggtccaacgc gcagctgggc gatgaagtaa aaatcgagta caagcatccg
     6121 ctgccaaaga aatttgacct ggtgattacc gccaaagcat acggcaataa cgccagccgt
     6181 cctattccgg tacgcgtagg caatgaagaa caaacccttg tgctgggcaa tgaagtgacc
     6241 accaccacac tgcatttcga taacccaacc gatgccgaca cactggtaat tgtgccgccg
     6301 gaacctgtct caaccaacga agggaatatc ctcggacact cgccgcgtaa gctcgggatc
     6361 ggcatggtgg aaattaaagt ggtggaacgt gaaggataat tcctgactga tgtgtaggtc
     6421 gcataaggtg cgacctacac accttctgcg gcctacaaaa catcattgag aaacagaaaa
     6481 aaacagcgcc caacacttcc ccggacgctg catggcaact ttaataatct cgccttatca
     6541 gaaagtaatt acacccttaa tcaactcacg attgttaatc acatcgcgct cgtaggtttc
     6601 tgccagcgtg gcgaacggat agcgatgggt taacatcatg tcagcagtga tttttccttc
     6661 cgccatcagt cgaccgactt tagcaaaatc ttccggcgtg gcgttgcggc tacccatcat
     6721 cgtcgtttct tttttatgga attccggatc ggagaactgc aactcacctt taaacaggcc
     6781 gacaaatacc accgtgccgc cgtgacgaat caaattcacg gtgttattca tcgcatgttg
     6841 attacctgtc gcgtcgatca ctttctgcgc cagcgaacca ccaaactgcg cccgtagctg
     6901 cgcgtcaaaa tcttcggctg acgggtccag taaaggtaat tccagacgcg ttgccacatg
     6961 ttcacggcgc gccggactgg tatccgccac caccacctgt gcgccatcgg ctttagcgat
     7021 tgccgccgcg cccagaccga ttggccccgc cccgaccacc agcacctgct cgccgggagc
     7081 aatggctgcg cgacgcaccg catgagcgct aatagcgaaa ggttcaatca atgctgccgc
     7141 ctgcgggtca ataccgtctg cgggcaaaat gttcgccacc ggcacgctca aatactcact
     7201 aaaaccgcca tcctgatgca cgccaatgac tgaaattttt tcacagcaat tggtacgccc
     7261 gcttttacac gccgggcatt gctgacaggc aacataaggg atcacagcaa cttgctgacc
     7321 atttttaaga tcagcaatat ttttacccag cccaacaatc tccccacata tttcatggcc
     7381 taaaacacgt ggataactaa aaaatggttg atttccaccc caggcatgaa tatcggtacc
     7441 gcaaatcccg acagacttaa tttttattaa tgcttcattg tcacccggaa taggtatctc
     7501 gcgttgtttc cagactaatt ctttcggctg ctggcaaatt aaaacattca tcgtagacat
     7561 gattcggctc tccatttttt gttgccttag atatcaatga aaatgaaaaa tggcagcgga
     7621 acggaaaatc tgttttgtga aaacacacgc ataaaaatgt tttaaatcgg gttttaatgg
     7681 actcacaagg aggaaaatat gagtcgttca caaaatttac gccacaatgt gattaaccag
     7741 gtcattgatg atatggcgcg gggtcatatc ccttccccac ttccatcgca aagtgcgctg
     7801 gcggaaatgt acaacatcag ccgcaccaca gtgcgtcata ttctcagcca cttacgcgaa
     7861 tgcggcgtct tgacgcaggt tggaaacgac tatgttattg cgcgtaaacc tgaccatgat
     7921 gacggtttcg cctgtactac cgcttcaatg agtgaacaaa acaaagtgtt tgggcaggct
     7981 tttttcacga tgataaacca gcgccagtta cgcccagggg aaacgttttc tgaactgcaa
     8041 ctggctcggg ccgcgggtgt cagcccggtt gtagtaagag aatatctttt aaaattcggc
     8101 cgttataatc ttattcacag cgaaaagcgc ggccagtgga gcatgaagca attcgatcaa
     8161 tcctacgccg agcagctgtt tgagctacgc gaaatgctcg aaacacattc acttcagcac
     8221 tttctcaacc tgcctgatca tgatccccgc tggctgcagg cgaaaaccat gctcgaacgt
     8281 catcggctac tgcgtgacaa catcggcaat agctttcgca tgttctcgca acttgacaga
     8341 gattttcact ccttactgct ttcagctgcc gacaatatct tttttgatca atcgcttgag
     8401 atcatctcag tcatctttca ttttcactat caatgggacg aaagcgatct taaacagcgc
     8461 aatatcattg ccgtcgatga gcatatgacc attctcagcg ctctgatttg ccgcagtgac
     8521 cttgatgcca cccttgcgct ccgtaaccat ttgaattcag ctaaacaatc aatgattcga
     8581 tccattaatg agaacacgcg ttatgctcat taagtacgga ttaaaaacag caaactatta
     8641 gcgggtgcac ggttttatca aaagataaat accgggaaat tcccgcttac ctatgctcac
     8701 aatcagtcga ctaaaaatgt ttttgtgagc ggtagtaaag tcctaaaact ttaacctgcc
     8761 gttaatgtca accttgcgta attcacaatt acgtaaacaa ttaaaattat aaatacagga
     8821 gtccggcgtg gaaaaagaaa atatcaccct cgatccgcgt tcttcattta ctccatcttc
     8881 gtcggcagat attcccgtgc caccagatgg attagttcaa cgcagtaccc gaattaaacg
     8941 cattcaaacc accgccatgt tgttattatt ttttgcggcg gtaatcaatt atctcgaccg
     9001 cagttcgctg tcggtagcaa atttaacgat tcgtgaagaa ttgggattaa gtgccaccga
     9061 aatcggcgct ttgctctccg tgttttcact cgcttacggg attgcgcaac ttccttgcgg
     9121 cccactattg gatcgtaaag gcccacgcct gatgctggga ctggggatgt tcttctggtc
     9181 actgttccag gcaatgtctg gcatggtgca caactttacg cagttcgtgt tggtgcgtat
     9241 cggtatgggg attggtgaag cgccgatgaa cccatgcggt gtaaaagtca ttaacgactg
     9301 gttcaacatc aaagagcgcg gacgcccgat gggcttcttc aacgcagctt ctaccattgg
     9361 cgttgccgta agcccaccga ttctggcggc gatgatgctg gtgatgggct ggcgcgggat
     9421 gtttattacc attggtgtac tggggatttt tctcgccatc ggctggtata tgctctatcg
     9481 caaccgcgag cacgtagaac tgactgccgt tgaacaagct tatctcaatg caggtagcgt
     9541 caatgcccgc cgagatccgc tcagttttgc cgaatggcgc agcctgttcc gtaaccgtac
     9601 aatgtgggga atgatgctcg gattcagtgg catcaactac actgcgtggc tgtatctggc
     9661 ctggcttcct ggttacctgc aaacagccta taacctggat ttaaaaagca cagggttgat
     9721 ggcggctatc cctttcctgt ttggggctgc cgggatgctg gtcaacggtt acgttactga
     9781 ctggctggtc aaagggggaa tggctccgat taaaagccgt aagatctgca ttattgccgg
     9841 gatgttctgt tctgccgcct ttacgctggt cgtaccgcaa gcgacaacat ccatgacagc
     9901 ggttctgctg attggcatgg cactgttctg tattcacttt gccggaacat cctgctgggg
     9961 cttgatccac gtcgcagttg cttctcgcat gactgcgtcg gtgggcagta tccagaactt
    10021 tgccagcttc atctgcgcct cttttgcgcc gatcattact ggttttattg ttgataccac
    10081 ccactcattc cgtctggcac taatcatctg cggttgcgtc accgcagcgg gggcactggc
    10141 gtacatcttc ctggttcgtc agccgatcaa cgacccacgt aaagattaat ctccttatgc
    10201 ccgataacat tttgcttatc gggcattttc atggcgatta aaatgtttcc cagttctcct
    10261 cgctatctgc cacggccatt ttacgcggcg cagctggcgt cacggttttt accacagccg
    10321 atgtttcacg ctgctgttgc tgaatccgga acactgccac tgcttcggtc aggcgactgg
    10381 cctgctcttc cagcgcggcg gcggcagcgg cagactcttc caccagcgcg gcgttctgtt
    10441 gagttacccg gtccatctca gcaaccgcta agccaacctg atcgatacca cggctctgct
    10501 catcagaagc agaagcaatt tcgcccataa tgtccgtcac gcgggtcacg gcgctgacaa
    10561 tctccgccat tgtttccccg gcgctttcga ccagcgtaga gccaacatcc actttcccca
    10621 ccgagtcttc aatcaggctt ttaatttcac gagccgcctg ggcgctgcgc tgggccagat
    10681 tacgcacttc tcccgcgacc accgcaaaac cgcgcccttg ctcacccgca cgcgcagcct
    10741 caaccgccgc gttcaaagcc agaatattgg tctggaaggc aatgccgtca attacgctga
    10801 taatatcggc gattttctgc gaactggtgg agatatcgcg catagtctgc accacgttat
    10861 ctaccacttt gccgccgcgt tgcgccgttt cagaagcact taacgccaga tggctggcct
    10921 ggcgcgcatt ttcggcgttc tgcttcaccg ttgcggtcag ttgctccatg ctggctgccg
    10981 tctcttccag cgaagcggcc tgttgctcgg tgcgcgaaga gagatcgtta ttgccggtgg
    11041 cgatttcgct ggcaccgcta tagatggcat tggccccgtt gcgcacatca ccgacggtac
    11101 gcatcagctc tccctgcata tggcgcaaac tctctgccag ttgccccatc tcattagagc
    11161 catccacctc aatcggtttc accagatcgc cgcctgcaat atgacgaatg ctgtcaatca
    11221 ggcgattcat tggcgctacc agcgaggctt taataccgaa ccagacggcg aagatgaccg
    11281 ccagtacgac gatcatcacg cccaccagaa tccacatcgc ctggctgtag gaggcattgt
    11341 tatcgctgac ggcgatatca tagagccgat cgttttgctc catgtaagcc acatactgct
    11401 tctcgaaacc gtcctgatat ccctgggtcg gctgatcaaa gaactcgttg attttgcctg
    11461 cacctaacag ttggatcagc tccgccagcg cattgtgata aatatcgtaa ttacgtttga
    11521 tctctgccgc tgcggcggtg ctctgacgcg ggtcacgcgg caacgcttcg taatccgccc
    11581 agtttttttc cgcctgtttc agcgaaatac tggcactctg catcagctca gcaacggttg
    11641 aaccgctacc aatattgttc tgatccatca tgtagcggat acccgcgcgg ttgagggtgt
    11701 tacgcgtctg caacaacgcg acccagctgc cattcagcgt ggattgctgc tggcgaatgg
    11761 tttgtaaaac agtgaaattt tctttgtcat tctttaaggc attaaagaac agaccgcctg
    11821 atgtcagttg taaaaggcca aaaacggcca aaaccagcag taagctggtc acgatcttga
    11881 tacgttttaa catggtttct ctttcctgtg gaccgatgca agattttcgg cctggaaagc
    11941 aaaaacttta tgaaaaggtg taacccacca tccatggaaa aaccaggcgc tggttatcag
    12001 atactaaaaa gttattcatt atcgcccggg atggcttctt gtcgggaatt gcccagagca
    12061 ataaattcct ctaacagatg ggttaactgt tgcattttct cggcagtaaa ctgagcttca
    12121 atctgccgat aggcttcttc aatctgtgtc tgagcgcggt tatacagcgc ctgcccctct
    12181 ttggttagcg agatatagag cttccgctga tcattgatcg gctttaatcg caacactaaa
    12241 ccgtcgcgtt ccatccgcgt gaggatcccg gtcaggcttg ggcgcaaaat gcaggcgcga
    12301 tacgccagat cgtgaaaatc cattgatggg ctttccgcca gaatacgcac gatgcgccac
    12361 tgttgctcag tcaaattatg ccgtttaacg attgggcgaa aataactcat cgccgcttct
    12421 cgcgcctgga gcaacgcaat ggttagtgag tcgtgcatta tctttcccct gaaaaattca
    12481 aaatcattaa tatagaaaca gttaatcgtc acgttaccgg gactgcgctg cccctgcaat
    12541 tcctgatttt atcaaaattg atgatatttt attaacttac tgatatttaa tattttatta
    12601 tctttaaatg taaatagttt gttaattaga tcacatttac atcacttact tttgccaaac
    12661 tacgcaatcg ggaataaaag caatcattaa catattaatg attaagatga tcgaatccga
    12721 ggagtggtaa tgaaaggcac tatcttcgcc gtagcgttga accatcgcag ccaacttgat
    12781 gcatggcagg aagcgttcca gcaatccccc tacaaagccc cgcctaaaac tgcggtctgg
    12841 tttattaaac cgcgcaatac ggtgattggt tgcggtgaac cgattccctt tccacagggt
    12901 gaaaaggtac tgagcggtgc gactgttgcg ctgattgtgg gaaaaacggc gacgaaagta
    12961 cgtgaagaag atgcggcaga gtacatcgcc ggatatgcgc tggctaacga cgtcagcctg
    13021 ccggaagaga gcttttaccg cccggcaatc aaagcaaaat gtcgtgatgg attctgcccc
    13081 attggcgaaa ccgtggctct cagcaatgtc gataatctga ccatctatac cgagatcaac
    13141 gggcgtcctg ccgatcactg gaataccgcc gatttacaac gtaacgccgc gcagttgctg
    13201 agcgccctga gcgaatttgc cacgctgaat ccaggcgacg ccattctgct cggcacgcca
    13261 caggcgcgcg tggaaataca gccaggcgat cgcgttcgtg ttctcgcaga aggtttcccg
    13321 ccgctggaaa atccggtagt ggacgaacgt gaagtgacca cgcacaagag cttcccaacg
    13381 cagccacacc cgcacggcac gctgtttgcc ctcggcctga actacgccga ccacgccagc
    13441 gaactggaat ttaagccacc ggaagaaccg ctggtgttcc tgaaagcgcc aaataccctc
    13501 actggcgata accagacctc cgtgcgtcca aacaatattg aatacatgca ctatgaagcg
    13561 gagctggtgg tagttattgg caaacaggcg cgtaacgtca gcgaagccga tgccatggat
    13621 tatgtcgcgg gctacaccgt gtgtaacgac tacgccattc gtgactatct ggaaaactac
    13681 taccgcccta acctgcgggt aaaaagccgc gacggactga cgccgatgct ttcaaccatc
    13741 gtgccgaaag aggcgatccc ggacccgcat aatctgaccc ttcgcacctt cgtcaacggc
    13801 gagttacgcc agcaaggcac caccgccgat ctgatcttca gcgtgccctt cctgatcgcc
    13861 tacttaagtg aatttatgac cctgaatccg ggcgacatga tcgccaccgg cacacccaaa
    13921 ggcttatctg acgtggtgcc tggcgatgaa gtagtggtgg aagtagaagg cgtgggccgc
    13981 ctggtgaacc gaattgtgag tgaggataca gcgaaatgaa aaaagtaaat cattggatca
    14041 acggcaaaaa tgttgcaggt aacgactact tccagaccac caatccggca acgggtgaag
    14101 tgctggcgga tgtggcctct ggcggtgaag cggagatcaa tcaggcggta gcggcagcga
    14161 aagaggcgtt cccgaaatgg gccaatctgc cgatgaaaga gcgtgcgcgc ctgatgcgcc
    14221 gtctgggcga tctgatcgac cagaacgtgc cagagatcgc cgcgatggaa accgcggaca
    14281 ccggcctgcc gatccatcag accaaaaatg tgttgatccc acgcgcttcc cacaactttg
    14341 aatttttcgc ggaagtctgc cagcagatga acggcaagac ctatccggtt gacgacaaga
    14401 tgctcaacta cacgctggtg cagccggtgg gcgtttgtgc gctggtatcg ccgtggaacg
    14461 taccgtttat gaccgccaca tggaaggtcg cgccgtgtct ggcgctgggc aataccgcgg
    14521 tactgaaaat gtcggaactc tccccgctga ccgctgacag cctgggtgag ctggcgctgg
    14581 aagccgatat tccggcaggc gtgctgaacg tggtacaggg ctacggcgca accgcagggg
    14641 atgcgctggt tcgtcatcat gacgtacgtg ccgtgtcgtt caccggcggt acggccaccg
    14701 ggcgcaacat catgaaaaac gccgggctga aaaaatactc catggaactg ggcggtaaat
    14761 cgccggtgct gatttttgaa gatgccgata ttgaacgcgc gctggacgcc gccctgttca
    14821 ccatcttctc gatcaacggc gaacgctgca ccgccggttc gcgcatcttt attcagcaaa
    14881 gcatctaccc ggaattcgtt aaacgctttg ccgaacgcgc caaccgtctg cgcgtgggcg
    14941 atccgaacga tccgaatacc caggttggcg cgcttattag ccagcagcac tgggaaaaag
    15001 tctccggcta tatccgtctc ggcattgaag aaggcgcaac cctgctggcg ggcggcccgg
    15061 ataaaccgtc cgacctgcct gcgcacctga aaggcggcaa cttcctgcgc ccaaccgtgc
    15121 tggcagacgt tgataaccgt atgcgagtcg cccaggaaga gattttcggg ccggtcgcct
    15181 gcctgctgcc gtttaaagac gaagccgaag gcttacgtct ggcaaacgac gtggagtacg
    15241 gcctcgcgtc gtacatctgg acacaggatg tcagcaaagt gttacgcctg gcgcgtggca
    15301 ttgaagctgg catggtgttc gtcaacaccc agaacgtgcg tgacctgcgc cagccatttg
    15361 gcggcgtaaa agcctccggc accgggcgtg aaggcggtga gtacagcttc gaagtgttcg
    15421 cggaaatgaa gaacgtctgc atttccatgg gcgaccatcc aattccgaaa tggggagtct
    15481 gatatgggta agttagcgtt agccgccaag atcacgcacg taccgtcgat gtatcactct
    15541 gaactgccag ggaaaaacca cggttgccgc cagggcgcga tcgacgggca taaagagatc
    15601 agcaagcgtt gccgggaaat gggcgtcgat accattatcg ttttcgatac ccactggctg
    15661 gtcaacagtg cttatcacat caactgtgca gaccattttg aaggcgtcta caccagcaac
    15721 gagctgccgc attttatccg cgatatgacc tacaactacg agggcaaccc ggagttgggg
    15781 cagcttattg ccgatgaagc cttaaagctc ggcgtgcggg caaaagcgca caacattccc
    15841 agcctgaaac tggaatacgg cacgctggtg ccgatgcgct acatgaatga agacaagcac
    15901 ttcaaagtgg tctccatttc ggctttctgc acggttcacg attttgccga cagccgcaag
    15961 ctgggcgaag cgattctgaa agcgatcgaa cagtacgacg gcaccgtggc ggtccttgcc
    16021 agcggttcgt tatcgcaccg ctttattgac gatcagcgtg cggaagaagg gatgaacagc
    16081 tacacccgcg agttcgaccg ccagatggac gagcgcgtgg tgaagttgtg gcgcgaaggc
    16141 cagttcaaag agttctgcaa tatgctgccg gagtacgccg actactgcta cggcgaaggc
    16201 aatatgcacg acacggtgat gctgctgggg atgctcggct gggataaata cgacggcaag
    16261 gtggagttta tcaccgagct attcccaagc tctggcaccg gtcaggttaa cgctgttttc
    16321 ccgcttcccg cgtaaggacg ttttatgccg cactttatcg ttaaatgcag tgacaacatc
    16381 cgcgaagaag ccgacctgcc ggggttgttc gccaaagtga atccgacgct ggcagccacg
    16441 ggtatttttc cgctggcggg tattcgcagc cgcgtgcatt gggtcgatac ctggcagatg
    16501 gccgacgggc agcgtgatta tgccttcgtg catatgacgt tgaaaatcgg cgcaggtcgc
    16561 agcctggaaa gccgccagca ggcgggtgaa atgctgtttg aactgattaa aacgcacttc
    16621 gccgccctga tggagagccg cctgctggcg ttgtcgtttg agattgaaga gctgcatccg
    16681 acgctgaatt ttaaacaaaa caacgtgcac gcattgttta agtagcgcgc agattgcccg
    16741 gtggcgctgc gcttaccggg cctacaaaac cccaaaccgt acaccgtagg ccggataagg
    16801 cgcagccgca tccggcaatg ccacaggata tcgctatgtt cgataaacac acccacaccc
    16861 tgatcgccca gcgtctggat caggcagaaa aacagcgcga acagatccgc gcgatctcgc
    16921 tggattaccc ggagatcacc atcgaagacg cttacgcggt gcagcgtgaa tgggttcgcc
    16981 tgaaaatcgc cgaaggtcgc acgctgaaag gccacaaaat cggcctgacc tcgaaagcga
    17041 tgcaggcaag ctcgcagatc agcgaaccgg attacggcgc gctgctggat gacatgttct
    17101 tccacgatgg cagcgatatc ccgaccgatc gctttatcgt gccgcgcatt gaagtggagc
    17161 tggctttcgt gctggcaaaa ccgctgcgtg ggccaaactg cacgctgttc gacgtctaca
    17221 acgccacgga ctacgtgatc ccggcgctgg agctgatcga cgctcgctgc cacaacatcg
    17281 atccggaaac ccagcgcccg cgcaaagtgt tcgacaccat ttctgataac gccgccaacg
    17341 ccggggtgat cctcggcggt cgtccgatca aaccagacga actggatctg cgttggatct
    17401 ccgccctgat gtatcgcaat ggcgtgattg aagaaaccgg cgtcgccgct ggcgtgctga
    17461 atcatccggc aaacggcgtg gcctggctgg cgaacaaact cgcaccgtat gacgtacaac
    17521 tggaagccgg gcaaatcatt ctcggtggtt cgttcacccg cccggttccg gcgcgtaagg
    17581 gcgacacctt ccacgtcgat tacggcaaca tgggttccat tagctgccgc tttgtttaag
    17641 gagagaacga tggaaaacag ctttaaagcg gcgctgaaag caggccgtcc gcagatcgga
    17701 ttatggctgg ggctgagtag cagctacagc gcagagttac tggccggagc aggattcgac
    17761 tggttgttga tcgacggtga gcacacgccg aataacgtgc aaaccgtgct cacccagcta
    17821 caggcgattg cgccctaccc cagccagccg gtagtacgtc cgtcgtggaa cgatccggtg
    17881 caaatcaaac aactgctgga cgtcggcaca caaaccttgc tggtgccgat ggtacaaaac
    17941 gctgacgaag cccgtgaagc ggtacgcgcc acccgttatc cccccgccgg tattcgcggt
    18001 gtgggcagtg cgctggcccg cgcctcgcgc tggaatcgta ttcctgatta cctgcaaaaa
    18061 gccaacgatc aaatgtgcgt gctggtgcag atcgaaacgc gtgaggcaat gaagaactta
    18121 ccgcagattc tggacgtgga aggcgtcgac ggcgtgttta tcggcccggc ggatctgagc
    18181 gccgatatgg gttatgccgg taatccgcag cacccggaag tacaggccgc cattgagcag
    18241 gcgatcgtgc agatccgcga agcgggcaaa gcgccgggga tcctgatcgc caatgagcaa
    18301 ctggcaaaac gctatctgga actgggcgcg ctgtttgtcg ccgtcggcgt tgacaccacc
    18361 ctgctcgccc gcgccgccga agcgctggca gcacggtttg gtgcgcaggc caccgccgtg
    18421 aagcccggcg tgtattaatg ccggatgcgc tgcgcttatc cggcctacac tcgcaccaaa
    18481 ccgtaggccg gataaggcgt ttacgccgca tccggcaaaa agccgtacca aatcgcggac
    18541 cggataaggc gttacgccgc atccgccata aaccttgtcg taccctacaa aaatcccatt
    18601 agaggaagaa aaatgagcga cacctcacct gccataccgg agagtatcga tccggcgaat
    18661 cagcataaag cgctgactgc cggacaacag gcggttatta agaagctatt tcgccgcctg
    18721 agcgtctttc tgttcgtgct gtttatcttc tcgttccttg atcgcatcaa catcggcttt
    18781 gccggactca cgatgggacg cgacctcggt ctgagcgcca ccatgtttgg cctcgctacc
    18841 accctgttct atgccgctta tgtcatcttc ggcattccca gcaacattat gctgagtatt
    18901 gtcggtgcgc ggcgctggat cgccaccatc atggtgctct ggggcatcgc ctctactgcc
    18961 accatgtttg ccactggccc caccagcttg tacgtactgc gtatactggt tggcattacc
    19021 gaagccggct ttctgcctgg tattctgctg tatttaacct tctggtttcc ggcctatttc
    19081 cgcgcccgtg ccaacgcctt gtttatggta gcaatgccgg taacgacagc gttgggctcg
    19141 atcgtttccg gctacatttt gtcgctggat ggcgtcatgg cattaaaagg ctggcagtgg
    19201 ctgtttttgc tggaaggctt cccgtcggta ttactcggcg tcatggtgtg gttctggctt
    19261 gatgactcac cggacaaagc taagtggctg acgaaagaag acaaaaaatg cctgcaagag
    19321 atgatggata acgatcgtct gacgctggtt cagccagagg gagccatcag ccaccacgcc
    19381 atgcaacaac gcagtatgtg gcgggagatc ttcactccgg tggtgatgat gtataccctg
    19441 gcgtatttct gcctgaccaa cacacttagt gcgatcagca tctggacacc gcagatcctg
    19501 caaagtttta atcagggcag cagtaatatc accatcggca tgctggccgc cgtaccgcag
    19561 atttgtacca ttctcgggat ggtctactgg agccgtcact cagatcgccg ccaggaacga
    19621 aggcatcaca ccgcccttcc ttatttgttc gctgccgcag gttggttact ggcttcggca
    19681 actgatcaca acatgatcca gatgctgggg atcattatgg cttcgaccgg atcattcagc
    19741 gcaatggcga ttttctggac aacaccggat cagtccatca gcctgcgggc acgagcgatc
    19801 ggtattgcgg tgatcaacgc cactggcaac attggttcag cgttaagtcc gtttatgatc
    19861 ggctggttga aagatttgac cggcagcttt aacagtggat tgtggtttgt tgccgcgctg
    19921 ctggtgattg gtgcggggat tatctgggcg attccaatgc agtcctcccg tccgcgagcg
    19981 accctgtaag gaacgacgat gtgtgaccgt cagattgcca atattgatat cagcaaagag
    20041 tacgatgaaa gcctgggcac ggacgatgtg cattatcagt cgttcgcccg catggcggca
    20101 ttttttggcc gccatatgct gccacatcgc cacgaacagt actttcagat gcatttcctc
    20161 aatagcggac agattgagct acagcttgac gatcatcgct actcggtgga agcgcccctg
    20221 tttgtcctga cgccgccgtc a
//
LOCUS       DAKISA010000084        19978 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.084, whole genome shotgun sequence.
ACCESSION   DAKISA010000084 DAKISA010000000
VERSION     DAKISA010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19978
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.084"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(1..114)
                     /locus_tag="OVW45_003021"
                     /pseudo
     CDS             complement(1..114)
                     /locus_tag="OVW45_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="receptor for colicin S4; incomplete; partial in the
                     middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
     gene            300..575
                     /locus_tag="OVW45_003022"
     CDS             300..575
                     /locus_tag="OVW45_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HCV5527414.1"
                     /translation="MTVFVLRGYIPHTTAKSVVGIGVSDNITTHSRVCGFFCVKHCHI
                     RIMVGRAGAEKSAPGFVMTGYANPVRLTTSLIGVRGGDHPINWSHHI"
     gene            572..1129
                     /locus_tag="OVW45_003023"
     CDS             572..1129
                     /locus_tag="OVW45_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCV5527415.1"
                     /translation="MNTAITFSNESPKVFFKDGRLVTTSQAVADYFDKQHKHVLAKID
                     TLDCSPEFTSANFSADVQTVEIGNGAERESRCYLITKDGFMFLVMGFTGKKAARLKEA
                     YIEKFNAMEEELHKRPPAAQNSPAPNNGCALLIHFDKHGQVEFTEKVPADAMVCTLER
                     FKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(1256..1426)
                     /locus_tag="OVW45_003024"
     CDS             complement(1256..1426)
                     /locus_tag="OVW45_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5527416.1"
                     /translation="MVRLPPVVMSAKSATARTTIAREDAEDHQVKLSAQKLEELLASM
                     VKDEVDRNDGIY"
     gene            complement(1541..1810)
                     /locus_tag="OVW45_003025"
                     /pseudo
     CDS             complement(1541..1810)
                     /locus_tag="OVW45_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            1867..2535
                     /locus_tag="OVW45_003026"
     CDS             1867..2535
                     /locus_tag="OVW45_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCV5527417.1"
                     /translation="MVKSMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTRVQGTVVMFSA
                     SGKK"
     gene            complement(2590..2688)
                     /locus_tag="OVW45_003027"
                     /pseudo
     CDS             complement(2590..2688)
                     /locus_tag="OVW45_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(2734..2916)
                     /locus_tag="OVW45_003028"
     CDS             complement(2734..2916)
                     /locus_tag="OVW45_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCV5527418.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGYEQIFEDKLSGTRTGRPG
                     LKRALKRLQKGDALVV"
     gene            3144..3905
                     /locus_tag="OVW45_003029"
     CDS             3144..3905
                     /locus_tag="OVW45_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCV5527419.1"
                     /translation="MAFDGSSDITLKASHVGAFALGKTGSTVANDKAVGWNWSSGAYN
                     ATISGASTLIIHFYMGEGSCPAAQFRINYKNGGIFYRSARDGYGFEADWSEFYTTRKP
                     SAGDVGALPLSGGQLNGALGIGTSSALGGNSIVLGDNDTGFKQKGDGNLDVYANNVHV
                     MRFVSGSIQSNKTINITGRVNPSDYGNFDSRYVRDVRLGTRVVQTMKKGVMYEKSGHV
                     ITGLGIIGEVDGDDPAVFRPIQKLINGTWYNVSQV"
     gene            3908..4441
                     /locus_tag="OVW45_003030"
     CDS             3908..4441
                     /locus_tag="OVW45_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCV5527420.1"
                     /translation="MQHLKNIVAGNPKTVEQYQLTKNFDVIWLWSEDGKNWHEELKNF
                     QEDTIKLAYTVEGIIIAIDKDVSAINPEGLSVVELPDITANRRADISGNWMFKDGAVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLQLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(4470..4997)
                     /locus_tag="OVW45_003031"
     CDS             complement(4470..4997)
                     /locus_tag="OVW45_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCV5527421.1"
                     /translation="MMHLKNIKAGNPKTPEQYQLTKKAGVVWLFCEDGKNWYEEQKNF
                     QPDTIKIAYDENNIIVAVSKDVSTINPEGLSIVEVPDITANRHADNNGKWMFLDGKVV
                     KREYTEQELQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTANPEWPQKPE"
     gene            complement(4999..7755)
                     /locus_tag="OVW45_003032"
     CDS             complement(4999..7755)
                     /locus_tag="OVW45_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCV5527422.1"
                     /translation="MTVKISGVLKDGTGKPVQNCTIVLKARRTSSTMVVNTVASENPD
                     EAGRYSMDVEHGQYSVTLLVEGFPPSHAGTITVYEGSRPGTLNDFLGAMTEDDVRPEA
                     LRRFEQMVEEVSRNASAVAQNTAAAKKSASEAATHATDAAASARAASTSAGQAASSAQ
                     SASSSAGTASTKAREAAKSAAAESSKSAAATSASAAKTSETNAAASQKSAATSASTAT
                     TKASEAATSARGAAASKEAAKSSETNASSSASSAASSATAAGNSAKAAKTSETNAKSS
                     ETAAGQSASAAAGSKTAAALSASAASTSAGQASASATAAGKSAESAASSASTATTKAG
                     KATEQATAAARSASAAKTSETNAKTSADNAASSKAAAASSASSAASSASSASASKDEA
                     TRQASAAKGSATTATTKASEAAGSATAASQSKVAAESAATRAETAAKRAEDIASAVAL
                     EDASTTKKGIVQLSSATNSTSESLAATPKAVKAVMGETNKKAPLNSPALTGTPTTPTA
                     RQGTNNTQIASTAFVMAAIAALVDSSPDALNTLNELAAALGNDPNFATTMTNALAGKQ
                     PKDATLTALAGLTTAAGKFPYFTGNDVASLATLTKVGRDILAKSTVAAVIEYLGLQET
                     VNKAGNAVQRSGDKMTGELKIGTVNALRIFNDTFGLIFRRSEDFLHFIPTAEGQGENG
                     NIGPLRPFAINLRTGAISVSHGAKIKGGLAIGATDNALGENSIVLGDNDTGFRQDGDG
                     IISFYSNGSRIGHIDELGLHLYKDIESNGSNFRLKSNYRHHITFANEDGRIRMFLWKD
                     NGGDGVHINNGSDGGGDFIFKTDGGFALGSGAQVASSGDIYGSVWENNWLSTWLHNHV
                     VRDIRLGSIEYKNVWRDYGFGDASGYVLTAAINSNADDIVDTVARRPIQKLIGGIWYN
                     VGSV"
     gene            complement(7907..8506)
                     /locus_tag="OVW45_003033"
     CDS             complement(7907..8506)
                     /locus_tag="OVW45_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCV5527423.1"
                     /translation="MRKVCAAILSAAICLSVSGAPAWASEHQSTLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDALGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMVGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVTIDLAYEGSGSGDWRTDAFIVGIGYRI"
     gene            complement(8574..10103)
                     /locus_tag="OVW45_003034"
     CDS             complement(8574..10103)
                     /locus_tag="OVW45_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCV5527424.1"
                     /translation="MRFSLRLTSGKGTDARLVTTAITADTEHRFSGLPLGEYTLTVRA
                     INSYGQQGEPATTTFRIAAPAAPSRIELTPGYFQITATPHLAVYDPTVQFEFWFSEKR
                     IADIRQVETSARYLGTALYWIAASINIKPGHDYYFYVRSVNTVGKSAFVEAVGRASDD
                     AEGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSNEITQTVNKKLKD
                     QSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIENTPAGMQSQVLLA
                     ADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNPPAFSLTPDGKLT
                     AKNADISGSVNANAGTLNNVTINENCQIKGKLSANQIEGDIVKTVGKAFPRDSRAPER
                     WPSGTITVRIYDDQPFDRQIVIPAVAFSGAKHERENNDIYSSCRLIVRKNGAEIYNRT
                     ALDNTLIYSGVIDMPAGRGHMTLEFSVSAWLVNNWYPTASISDLLVVVMKKATAGISI
                     S"
     gene            complement(10109..11752)
                     /locus_tag="OVW45_003035"
     CDS             complement(10109..11752)
                     /locus_tag="OVW45_003035"
                     /inference="COORDINATES: protein motif:HMM:NF024939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCV5527425.1"
                     /translation="MEVTKAKPVTRTITSANIDRLRVTFGVQSLVQTTSKGDRNPTSV
                     RLLIQLQRNGNWVTEKDVTINGKTTSQFLASVILDNLPPRPFNIRMVRETADSTTDQL
                     QNKTLWSSYTEIIDVKQCYPNTAIVGLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDP
                     EKRTYSGIWDGSLKPAYSNNPAWCLWDMLTHPRYGMGKRLGAADVDKWALYAIGQYCD
                     QTVPDGFGETEPRMTFNAYLAQQRKAWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDV
                     VWPYTNSDVVVDDNGVGFRYSFSALKDRHTAVEVNYTDPQNGWQTSTELVEDPEAILR
                     YGRNLLKMDAFGCTSRGQAHRAGLWVIKTELLETQTVDFTLGSQGLRHTPGDIIEICD
                     NDYAGTLTGGRVLSIDAATRTLTLDREVTLPETGAATVNLINGSGKPVSVDITEHPAP
                     DRIQVSTLPDGVETYGVWGLSLPSLRRRLFRCVSVRENTDGTFAITAVQHVLEKEAIV
                     DNGARFEPQSGSLNSVIPPAVQHLTASIWRRRNGTRRGW"
     gene            complement(11822..12025)
                     /locus_tag="OVW45_003036"
     CDS             complement(11822..12025)
                     /locus_tag="OVW45_003036"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5527426.1"
                     /translation="MGKGGGKAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAV"
     gene            complement(12369..13049)
                     /locus_tag="OVW45_003037"
     CDS             complement(12369..13049)
                     /locus_tag="OVW45_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCV5527427.1"
                     /translation="MATTNAFSLASPPLARICLHGDLQRFGRRLSLYVNTAAEAIRAL
                     SMQMPGFRRQMNEGWYQIRIAGYDTAPEAVYARLHEQLGEGTVIHIVPRLAGAGKGGL
                     QIVLGAAAIVGSFFTAGASMALWGSALAAGGFSATTMLFSLGASMILGGVAQMLAPKA
                     KTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGGGK
                     VVVIGRQG"
     gene            complement(12947..13690)
                     /locus_tag="OVW45_003038"
     CDS             complement(12947..13690)
                     /locus_tag="OVW45_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCV5527428.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGTEMPDFHREDDWWRNGQNLYLDNMAVT
                     GFYRVPLSSAQAGDILLCCFGASVPNHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGIYNDLAAASACM"
     gene            complement(13701..14399)
                     /locus_tag="OVW45_003039"
     CDS             complement(13701..14399)
                     /locus_tag="OVW45_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCV5527429.1"
                     /translation="MQDIREESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIDGSGFEMNGRGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSQLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCELRRNV
                     GNFGGFLSINKLSQ"
     gene            complement(14399..14728)
                     /locus_tag="OVW45_003040"
     CDS             complement(14399..14728)
                     /locus_tag="OVW45_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCV5527430.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(14725..17298)
                     /locus_tag="OVW45_003041"
     CDS             complement(14725..17298)
                     /locus_tag="OVW45_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001621422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCV5527431.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDARKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLADAITLPMVGVTSLAVATGALVYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQARESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLMAMARQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIA
                     TKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADMLASAQKAFDE
                     ADKKWQWYQSRSQRRGKTSSFRANLQGAWDDRENARLGLAAATLQSDMEKAGELAARD
                     RAERESSQLKYTGEAQKAYERLLTPLEKYTARQEELNKALKDGKILQADYNTLMASAK
                     KDYESTQKKPSGVKVSAGERQEDQAHAALLALETELRTLEKHSGANEKISQQRRDLWK
                     AENQYVVLKEAATKRQLSEQEKSLLAHEKETLEYKRQLAELGDKIEHQKRLNELAQQA
                     ARFEQQQSAKQAAISAKARGLTDRQAQRESEEQRLREVYGDNPAALAKATSALKNTWS
                     AEEQLRGSWMAGMKSGWGEWAESATDSFSQVKNAATQTFDGIAQNMAAMLIGSEQNWR
                     GFTRSVLSMLTEIFLKQAMVGIVGSIGSAIGGAFGGGASASTGTAIQAAAANFHFATG
                     GFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMR
                     RAEGINFNQNNHVVIQNDGPNGRAGPQLMKAVYEMARKGAQDELRLQLRDGGMLSGSG
                     G"
     gene            complement(17279..17692)
                     /locus_tag="OVW45_003042"
     CDS             complement(17279..17692)
                     /locus_tag="OVW45_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCV5527432.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWHRFYRTHYFQDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGVRFGGDGGRD
                     ILSSADVADVMVDDAALMMASAGIPGGVRYVPAGW"
     gene            complement(17719..18150)
                     /locus_tag="OVW45_003043"
     CDS             complement(17719..18150)
                     /locus_tag="OVW45_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCV5527433.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTQSPSMNEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMIEAVRPDTDITEVAKNNTLTDDDFSAGKSSTAS"
     gene            complement(18164..18916)
                     /locus_tag="OVW45_003044"
     CDS             complement(18164..18916)
                     /locus_tag="OVW45_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCV5527434.1"
                     /translation="MATPNPLEPVKGAGTTLWVYNGKADAYANPLSDDDWQRLAKVKD
                     LTPGEMTAESYDDNYLDDEDADWSATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITP
                     VSAIKVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSADPSKATISVKDMTITVNG
                     VATGKVQIPVVSGNGQFAAVAEVTVTEAGAAG"
     gene            complement(18924..19319)
                     /locus_tag="OVW45_003045"
     CDS             complement(18924..19319)
                     /locus_tag="OVW45_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCV5527435.1"
                     /translation="MKHTDIRAAVLDALEQHEHGATLFDGRPVVFDEEDFPAIAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAMTAIPALAGLITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            complement(19316..19891)
                     /locus_tag="OVW45_003046"
     CDS             complement(19316..19891)
                     /locus_tag="OVW45_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCV5527436.1"
                     /translation="MAIKGLDQAIDNLSRVRKNAIPAASAMTINRVATTAINQSSSQV
                     ARETRVRRKLVKERSRLKRATVRNPNARIIVNRGDLPVIKLGIRMLGRRPNSILKAGQ
                     HRYQRAFIQRLNNGRWHVMQRLPQARYEEGNDDKGRKKRNRLPIQVVKIPMAAPLKQA
                     FDENVDRIRRERLPKELAYALKQQLRIAIKR"
     gene            complement(19906..>19978)
                     /locus_tag="OVW45_003047"
     CDS             complement(19906..>19978)
                     /locus_tag="OVW45_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCV5527437.1"
                     /translation="PDDGGSCYLWLNRGQPPAVNRRR"
BASE COUNT         4466 a         5764 c         4744 g         5004 t
ORIGIN      
        1 tcctgctgag aacataaaca cccacggatc gaggcgtacg ctatcatgtt gctgtgcacc
       61 gcccagttta taattggcga tggtatcgat atccatgtac cacactgaca tgttttaaaa
      121 aatgcaataa gacattgtat ctgaaagaaa aaacaggatt ttgaggcgct tttttatacc
      181 gtttttccgt gtttttaact gcctgatttt attgaaatct tttttagttt tgcagaaagg
      241 attttttgct cgatcgcttt aattttatac attcctgata acggaaattt ttttgcccgt
      301 tgacagtttt tgttctccgg ggctatattc ctcacacaac cgcaaaatcg gttgtcggga
      361 ttggcgtctc ggataacatc acaacgcata gccgcgtttg cggttttttt tgcgttaagc
      421 attgccacat tcgcattatg gtggggcgtg caggggcgga gaaatccgcg ccgggttttg
      481 tgatgaccgg ttacgccaac cctgtacgtc tcaccacctc cctgattggc gtcagaggtg
      541 gtgatcatcc aattaattgg agtcatcaca tatgaataca gctataactt tttctaacga
      601 gtcaccaaaa gtctttttca aagatggtcg cctggttacc acctctcaag ctgttgccga
      661 ctacttcgat aaacaacata aacacgtttt agctaagatt gacacactcg actgctctcc
      721 cgaatttaca tcagccaact tttcggccga tgttcagact gtcgaaattg gcaacggcgc
      781 agaacgtgaa tcacgatgct acctgatcac aaaagacggc ttcatgttcc tcgtaatggg
      841 atttactggc aaaaaagcag ccagactaaa agaagcttac attgaaaaat ttaacgcaat
      901 ggaagaagaa ctccacaaac gtcctcctgc agcacaaaac tcccccgccc caaataatgg
      961 atgcgcatta ctgatccact tcgataaaca cggtcaggtc gagttcacag aaaaagtccc
     1021 cgccgatgcg atggtatgca ctctggaacg gtttaaattt tatctggagc aacatgggtg
     1081 gatcgttgcc cgtaaagaac aattggtgga aaggttgatg cggttttaat gaatgcaaaa
     1141 ccccggatga tcaccgaggt taattttatc gagcgtgatt gccgctaatc gccatcgctc
     1201 tgattgagcg taaatcattc aggttattca gttcttcctt ctgctctcgc tgacgtcaat
     1261 aaatcccatc attgcgatca acctcatcct taaccattga tgcgagcagt tcttccagtt
     1321 tctgcgctga cagctttacc tggtgatctt cagcatcttc ccgggctatt gtggttcgcg
     1381 cagtggctga ttttgctgac atcaccacag ggggcagacg gaccattgaa ccatggcctc
     1441 cggaacaaat aagggcaaaa ataacaacca ctatcgaaag ctcacaaact aaccgcagca
     1501 cgttcctgca tacgacgtgt ctgcggcata atcccaatga ttactccctg acaggatttg
     1561 caggccactc aatatcaggt gcagttgatg tatcaacacg attcaacaat acccgatatt
     1621 tattccatgc ctccagcaac gatctttctt cctccgttgc gatttccaga tctacagcat
     1681 cctgcagtgg cgcaatatac tcactaaatt cctggatgta gaactgtgtg gtgacggtct
     1741 tccagccatt cggctcctgc tgtatcgaag catactaggc tatttcaata tcgctatgct
     1801 gcggcagcat ttaacccctt gtaattcatc gccataattg atttaattca caaataaaac
     1861 tataacatgg tgaaatcaat gaaaaaaaac acagatgatg gggctaaaat ttacacacca
     1921 cttaccctaa agctttatga ctggtgggtt ttgggagtat caaatcggct tgcatgggga
     1981 tgtcctacaa aggaacacct tcttccacac tttctggaac atttaggtaa caaccatctg
     2041 gatattggtg ttggaactgg gttttacctt actcacgtac ctgagagtag tctgatatct
     2101 ttaatggatt tgaacgaagc tagcctgaac gcggcatcta caagggctgg ggaatcaaaa
     2161 attaaacata aaattagcca tgatgttttt gaaccttatc ccgcggcgtt acatggtcaa
     2221 tttgattcca tttccatgtt ttaccttctt cactgcctgc ctggaaatat atctacaaaa
     2281 agctgtgtaa tacgcaatgc ggcgcaggcc ttaactgacg atggaactct atacggagcc
     2341 acaattcttg gcgatggagt tgtgcacaat agcttcggtc aaaaactgat gcgcatttac
     2401 aatcagaaag gcatcttttc aaacacaaaa gattccgaag aaggcttaac acatatactc
     2461 tcagagcatt tcgagaatgt taaaaccagg gttcaaggta ctgtagtaat gttttccgct
     2521 tcagggaaaa aatagcatcc aaccgcagca cgttcttgct taagacgtgc tgcggcataa
     2581 tcccaatgat tactccctga cagggttcgt aggccactca atatcaggtg cagttgatgt
     2641 atcaacacgg ttcagcaaca cccgatactt tttctaggct tccaccacca gcacgacaag
     2701 atgccgcata cagtgaccca gtcagtccag ttttcagaca accagtgcgt cacctttttg
     2761 aaggcgcttt aaagcacgtt ttaatccagg tcggcctgtc cttgttccgc ttaatttatc
     2821 ttcaaatatt tgttcatatc ctgcacaaac aagagcgttt cgttgcaggt ctgtattctg
     2881 gtcatttgtt gataccctta catagccaat cagcacgctg aatctcccgt ccaaaagcac
     2941 aaatcatgcc atgcaggcca gaaaccgcca ttatctaaaa cctcggttta cgagaactcg
     3001 gcacaagcgg ggagaaaata ccgttactca gtacagcgaa tacctggact aatcgacaaa
     3061 cattcagcgg tggcctttct ggtgaactat ccggcaatgc ttctacagct gcaaaattaa
     3121 aaactgccag gaaaataagc aatgtggctt ttgatggttc ctccgatatc acattaaaag
     3181 caagtcatgt tggtgcgttt gccttaggga aaacaggaag caccgttgcg aatgataaag
     3241 cagttggatg gaactggagt agcggagcct ataacgcaac tattagtggt gcatcaacgt
     3301 taattattca tttttatatg ggagaaggaa gttgtcctgc agctcagttt cggattaatt
     3361 ataaaaatgg cggtattttt tatcgttcag cccgtgatgg ttatggtttt gaagccgact
     3421 ggtccgaatt ttacaccacc agaaaacctt cagcaggaga tgttggtgca ctgccgttat
     3481 ctggtggtca actgaatggt gcactgggta tcggaacatc cagtgctctt ggcggtaatt
     3541 cgattgtatt gggtgataat gacacgggct ttaaacaaaa aggcgatggt aatctggatg
     3601 tttatgctaa taacgtccat gttatgcgct ttgtctccgg aagcattcaa agtaataaaa
     3661 ccataaatat tacgggacgt gttaatccct cggattacgg taactttgat tcccgctatg
     3721 tgagagatgt cagacttggc acacgagttg ttcagaccat gaagaaaggc gtgatgtatg
     3781 aaaaatcagg ccatgtaatc acggggcttg gtattatcgg tgaagtcgat ggtgatgatc
     3841 cggcagtatt cagaccaata caaaagttaa ttaacggaac atggtataac gtatcgcagg
     3901 tataatcatg cagcatttaa aaaacattgt cgcaggtaat ccaaaaaccg ttgaacaata
     3961 ccagctaaca aagaattttg atgttatctg gttatggtcc gaagatggaa aaaattggca
     4021 tgaggaatta aaaaactttc aggaggatac aataaaactg gcttacacag ttgagggaat
     4081 aattattgct atagacaagg atgtatcagc aattaatcca gaaggtttaa gtgtcgttga
     4141 gttgcctgat attacagcaa atcgccgggc tgatatttcg ggcaactgga tgttcaaaga
     4201 tggtgcggtg ataaagcgaa cttataccga ggaagagcaa aggcaacaag cggaaaatga
     4261 aaagcagagc ctgctgcagc tcgtcaggga taaaacccag ctgtgggact ctcagctaca
     4321 gctgggcatc atttccgatg agaataaaca aaaattaacc gagtggatgc tctatgcgca
     4381 gaaggttgaa tccacagaca cctccagcct gccagtaacg tttcccgaac aaccagaatg
     4441 agagaaggcc cgctatcggg ccttaatttt tattcaggct tttgtggcca ttcaggattt
     4501 gccgtatcca cacggctgac cagaacactg tagcgttccc atgcttccag tcgtgtgcgt
     4561 tcctcgtctg ttgccatatt cagcctgaca gcgcgttcca gcggctggat gactgattca
     4621 gcttcggaaa gcaatgcggc cttttgtgat tcggcctgtt gttgcagttc ctgttctgta
     4681 tattccctct ttactacttt accatccaga aacatccatt ttccgttatt gtctgcgtga
     4741 cgatttgccg taatatcagg aacttcaact atgcttagcc cttcaggatt aatagttgaa
     4801 acgtctttac tgacagcaac gatgatgttg ttctcatcgt aagcaatttt tatggtgtct
     4861 ggctgaaaat tcttttgttc ctcataccag tttttgccat cttcgcaaaa gagccatacc
     4921 acgcccgctt ttttagttag ctggtattgt tccggtgttt tcggattccc ggccttaata
     4981 tttttcaggt gcatcatctt aaacactccc cacgttgtac catattcccc caatcaattt
     5041 ctgaattggc cttctggcaa cagtgtcgac aatatcatcc gcattgctgt taattgcggc
     5101 tgttaaaaca taacctgacg catcgccaaa gccgtagtcg cgccatacgt ttttatattc
     5161 aatgctgcca agacgaatat cccgaacgac atgattatgc agccatgtgc ttaaccagtt
     5221 gttttcccac actgaaccat aaatatcgcc actagacgca acttgcgcac cacttcccag
     5281 cgcaaatccc ccatctgttt taaaaatgaa atcaccacca ccatctgaac cgttattaat
     5341 atgaacacca tcaccaccgt tatctttcca caaaaacata cgaattcttc cgtcttcgtt
     5401 ggcgaatgta atgtggtggc ggtaattact ttttaatcta aaattgctgc cattagattc
     5461 aatatcttta taaagatgta atcctaactc atcaatatgt ccgatgcgcg aaccatttga
     5521 atagaagcta ataataccat ctccatcttg cctaaatccg gtgtcgttat ctcccagaac
     5581 aatggagttt tcacccagtg cgttatcagt agctccaata gctagacctc ctttaatttt
     5641 ggccccgtgg ctgacagata tagcacctgt tctcagattt atagcgaatg gccttaatgg
     5701 gccgatatta ccgttttcgc cttgtccttc agccgtcgga atgaaatgaa gaaaatcttc
     5761 tgaacgacgg aaaattagac cgaaggtatc attgaaaatt cgcagcgcat tcaccgtgcc
     5821 aattttcagt tctccggtca ttttatcgcc ggaacgctga acggcgttac cagccttgtt
     5881 taccgtttcc tgtaaaccga ggtattcgat aacggcagca acggtcgatt tcgcaagaat
     5941 atcccgcccg acttttgtca gggttgccag gctggcgaca tcattccccg taaaatacgg
     6001 aaacttgcct gccgcagtag taagtccggc cagcgccgtc agggtggcat ctttcggttg
     6061 cttacccgca agcgcgttag tcatggtggt cgcaaaattc gggtcgttgc ccagcgccgc
     6121 agccagctcg ttcagcgtgt tcagtgcatc aggtgacgaa tctacaaggg cggcaatcgc
     6181 agccataacg aaagccgtgc ttgcgatttg ggtattattc gttccctgtc gcgcagttgg
     6241 tgttgttggc gttccggtca gtgcaggact atttaagggc gctttcttgt tcgtttcacc
     6301 cattaccgcc ttaaccgctt ttggcgttgc cgccagtgac tcggaagtgc tgttggtcgc
     6361 actgctgagc tgtactatcc cctttttcgt cgtgctcgca tcctccagcg ccacggcgga
     6421 tgcaatatcc tctgcccgtt ttgctgctgt ctcggcgcgc gttgccgcgg attcagcagc
     6481 aactttgctc tgagatgcag ccgtcgcact gcctgccgcc tctgatgctt tcgttgttgc
     6541 tgtcgtggca ctacctttcg ctgctgacgc ttgtctggtc gcctcatctt ttgaagcaga
     6601 cgcagatgat gccgatgacg ccgctgaact ggctgacgat gcggctgctg ccttagagga
     6661 agcagcattg tctgctgaag tctttgcatt tgtttcagag gtttttgctg cagaagcaga
     6721 cctcgctgct gcagtggctt gctcagtggc tttgccagcc ttcgttgtgg ctgttgaagc
     6781 ggatgatgcg gcgctttctg ccgattttcc ggcggcggtg gcactggctg aggcctgccc
     6841 ggcacttgtt gacgcggcac tggcagataa tgcagccgct gtttttgaac ctgccgcagc
     6901 tgaggcgctc tgtcccgctg ctgtttcaga agacttagcg ttcgtctcgg acgtttttgc
     6961 cgccttcgcg gaatttcctg ccgccgttgc cgaggaagct gcactactgg cacttgatga
     7021 tgcattcgtt tctgaagatt tcgctgcctc ttttgaggcc gccgcacccc gtgccgaggt
     7081 ggcagcttct gacgccttcg tggtcgctgt ggatgcagaa gtggctgccg atttttgtga
     7141 tgctgcggca ttcgtttctg acgttttcgc ggcactggcg ctggtagctg ccgcgctttt
     7201 tgatgactct gcagcagcac tttttgctgc ttcacgggcc tttgtcgatg ccgttcctgc
     7261 gctggaagac gctgactgag ccgacgacgc ggcctgtccg gctgacgtgc tggcggcacg
     7321 tgctgaggct gcagcatcgg ttgcatgagt tgccgcctcg ctggctgatt ttttcgcggc
     7381 tgccgtattc tgtgcaaccg cggaggcgtt acgtgacacc tcttccacca tctgctcaaa
     7441 gcggcgcagt gcctccggtc ggacatcatc ctccgtcatg gcaccgagaa aatcattcag
     7501 cgtacctggt ctggaacctt catagacggt aatggtcccg gcatgtgaag gcggaaaacc
     7561 ttcaaccagc agggtgacgc tgtactgacc atgctcaaca tccatgctgt aacgtccggc
     7621 ttcatccgga ttttcagagg ccaccgtgtt caccaccatc gtgctgctgg ttcgtctggc
     7681 cttcagcaca atggtgcagt tctgtactgg ttttcctgtg ccatctttaa gcacgccaga
     7741 aatttttact gtcatacttt tccaccaata aaaaaagccc gcagcagtga cgccacgggc
     7801 ttcaggacag tgtaacttta cgtttcctca aacgcagttc accccataag gtggatgaac
     7861 ctgcgtatca taacaatatt tacagaagat aaatcggcgt ctgttgtcag atacggtatc
     7921 cgataccaac aataaatgca tccgttcgcc agtcaccact accggaacct tcataagcaa
     7981 ggtcaatggt cacggattcg gtcgggttaa actgcacgcc agccccccac gccagagacg
     8041 tgttgctgtg gcgaccgtca tcacttccgg tcagcacatc gtgcgttttc cccttgttgt
     8101 cagttacgcg gagataatcc ccggagaaag tcgacacacg gctgtaagcc acacccacca
     8161 tcgcatacgc gctgaaccat tcattcacgc gtacagacgg ccccgccatc acgctgaacc
     8221 agcggttacg cacggaatct tcatgccagc gggtatcgct gtagtgcgtt ttttgctcat
     8281 cctcagcatt ggcataactg aaggacgtaa tcagccccag cgcgtccgta aactcataac
     8341 ggtatttcac gttaatcccg ttcagattat cgctgccggg agcgttcgta cgggcatgaa
     8401 gataccccgc gctcagtgtg gactgatgtt cagacgccca tgcaggcgca ccggatacgg
     8461 acagacagat ggctgcggac aaaatggctg cacaaacttt acgcataatt acctctcgct
     8521 tttctgcaat aaaaaaggcg ccatttctgg cgcccgtata tgggttataa aattcagctg
     8581 atactgatgc ctgcggtggc tttcttcatc accacaacca gcaaatcgct gatacttgct
     8641 gtgggatacc agttattcac cagccatgct gacaccgaaa actccagcgt catgtggccg
     8701 cgaccagctg gcatatcaat aacaccactg taaatcagcg tattatccag cgcggtacgg
     8761 ttataaattt cagcaccgtt tttccgtact atcaggcggc atgacgaata aatatcgtta
     8821 ttctcccgct catgtttagc gccgctgaat gccaccgccg gaataacaat ttgcctgtca
     8881 aaaggctgat cgtcataaat cctgacggta atggtccctg atggccaccg ctccggtgcc
     8941 cgggagtccc gcggaaaagc cttacccact gttttgacta tatcgccttc aatctggttg
     9001 gctgacagtt tccccttaat ctgacagttc tcattaattg tgacattgtt gagcgtcccg
     9061 gcgttcgcat tcacactgcc actgatatct gcatttttag cggtcagttt tccgtccggt
     9121 gtcagggaaa atgccggagg attaccgccg ctggtaatgg tgggagccgt caggcgtttc
     9181 aggaacacgt cgttcatgaa tatctgatcg ccctgaccaa caaacatcgg ctttgtgttg
     9241 ccattcgcag gattaatcat cgcaatcctg tctgccgcca gcagcacctg actctgcatt
     9301 cctgctggcg tattctcaat accggcaccg atacccgcaa tataaaggcg tccgtccttc
     9361 atctgttgca gcttcacagc ccacatgctg ttcagattat tatttgtatc aacctgaacc
     9421 ttctgtatct gctggattgc cgcactctgg tctttcagtt tcttattgac agtctgcgtg
     9481 atttcattgc tgacatccgt aatggacgtc ctgatttcag ccaggtcagg cgcaagctga
     9541 ccgttatcaa tctgcgtcca cagctcctga gccagatgtg ttttccctat ctctcctttg
     9601 aaaaaatcca ggtaaccttc cgcatcatcg ctcgcccgac cgacagcctc cacgaatgcc
     9661 gatttgccaa cggtgttcac actgcgaacg taaaaataat aatcatggcc cggtttgata
     9721 ttgatactgg cggctatcca gtacagcgcc gtgccaagat agcgggcgct ggtttcaacc
     9781 tgcctgatat ccgcaatccg cttttccgag aaccagaact caaactgtac cgtcgggtca
     9841 taaacggcaa gatgcggcgt tgcggttatc tgaaaatagc ccggcgtcag ctcaatccgc
     9901 gacggtgctg ccggtgcggc aatccggaag gtggtggtgg caggttcacc ctgctggcca
     9961 tagctgttta tcgcccgcac cgtcagggtg tattccccga gcggcaggcc gctgaaacgg
    10021 tgctccgtgt ctgcggtgat ggcggtggtc accagtctgg catccgttcc cttaccactg
    10081 gtcaggcgca gactgaagcg cacacccttc accacccgcg gcgtgtccca tttcgcctgc
    10141 gccagatact ggccgtcagg tgctgcactg ccggtgggat gacgctgttc agggaacctg
    10201 actgcggctc aaagcgggca ccgttatcca cgatggcttc tttttccagt acgtgctgca
    10261 ccgccgtgat ggcaaaggtg ccgtccgtgt tttcccggac ggagacacag cggaacaggc
    10321 gacggcgcag tgacggcagg gagagtcccc acaccccgta tgtctccaca ccatcaggca
    10381 gggtactgac ctgtatccgg tccggcgcgg ggtgttcggt gatgtccaca ctcaccggct
    10441 taccgctgcc gttaatcagg ttcaccgtgg cggcaccggt ctccggaagt gtcacttcac
    10501 ggtccagcgt cagagtgcgg gtggcagcat caatggacag gacacgtccg ccggtcaggg
    10561 tcccggcata gtcgttatca cagatttcaa tgatgtcacc gggtgtgtgc cgcagcccct
    10621 gagacccgag cgtgaaatcc accgtctgcg tttccagcag ttcggtcttt atcacccaca
    10681 gtccggcacg gtgggcctga ccgcggctgg tacagccgaa cgcgtccatc ttcagcaggt
    10741 tgcgtccgta gcgcagtatg gcttccgggt cttccaccag ttccgtggag gtctgccagc
    10801 cgttctgcgg gtcggtataa ttcacctcca ccgccgtgtg ccggtccttc agggcactga
    10861 agctgtagcg gaaacccacg ccgttatcat ccaccaccac atcgctgttg gtgtacggcc
    10921 acaccacatc cgacgggcgg tcctgaacaa acgtcagcgt ctggccgttc cataccggca
    10981 tacagcgcat cgccgagcag aaatcactga gcacgtccca tgccttacgc tgttgtgcca
    11041 ggtacgcatt gaaggtcatc cgcggctcgg tctccccgaa accatccggg accgtctggt
    11101 cgcagtactg cccgatggca tacagcgccc acttgtccac atccgccgcc cccagacgtt
    11161 ttcccatgcc gtagcgcggg tgagtcagca tgtcccacag acaccaggcc ggattgttgc
    11221 tgtatgccgg tttcaggctg ccgtcccaga taccactgta cgtgcgtttt tccgggtcat
    11281 agtttgacgg cacctggatg atgcgaccgc ggatatggta gttcaccgtc atctgctggc
    11341 cgccgaactg ctccgcatcc acctgcagcc ccacaatggc cgtgttcggg tagcactgtt
    11401 tcacatcgat gatttcggtg tatgacgacc acagcgtctt attctgcagc tggtccgtgg
    11461 tgctgtccgc tgtctcccgg accatccgga tgttaaaagg acggggaggc agattatcca
    11521 gaatcaccga cgccagaaac tgcgaggtgg tcttgccgtt aatggtgaca tccttttctg
    11581 tcacccagtt accgttacgc tgtaactgaa tcagcaggcg gacggatgtc gggtttcggt
    11641 caccctttga cgtggtctgc accagtgact gcaccccgaa ggtaacccgc aggcggtcaa
    11701 tgttcgcgga cgtaatggtg cgcgtcaccg gttttgcctt cgtcacttcc acgcccagtc
    11761 cggtttcagc accggaggac tcaaagcctt ccggtggtgt ctgctcctgc tccccggcgc
    11821 gtcagaccgc cgtcacaccg tgtatcacgg gattaccgtc cgtgtccgtc agcggggttt
    11881 tgttcaccag gatactctgc agtcccttca ccggaccttc tatcggtccc tcaccaatcg
    11941 catcaatcac gctcatcatc tgcgtggatt tgagattatc cttcgcctca cgaggcgtgt
    12001 gtgccttacc gccacctttt cccatacagc cttcccctga ataaattaac cgccacttgc
    12061 cattccgtac agaagtcgga tatccttcgc ccgaaaagca tgaaacacat ttctgccatg
    12121 ctaaagagaa accccggtat cagcagatac cggggttttc tttcatgccc accgataatc
    12181 ctgttggtta aaaccggtaa tggcataaaa attctgaata tcttcacatt ttcacaaact
    12241 gactgtggcg cgtataattt ctctgcgtta atttttttgt cgtgatataa gaataattcc
    12301 ttacacttaa tcttcgtaac tctcccgcag ttcctgtccg cgatcactgc gggatttttt
    12361 tattcttttt acccctgccg cccgataacc acgacctttc cgcccccgcc ttcatcacgg
    12421 gtgctgatgt cctgggatat acggcgggag ccaaccagca tttccccgta aggcaccggc
    12481 atcgggttcc cctgggcaat catgttatcc agtgaggaaa agtacgtgtt ctgtctgccg
    12541 ttatccgttg cgcggtaatc cggtgttttt gccttcgggg ccagcatctg ggccacaccg
    12601 cccagaatca tgctggctcc aagtgaaaac agcatcgtgg tggcagaaaa accaccggct
    12661 gccagggctg aaccccataa cgccattgat gccccggcag tgaagaaaga gcccacgatg
    12721 gctgccgccc ccagcacaat ctgcagtcca ccctttccgg ccccggccag tcgcggcaca
    12781 atgtggatga ccgttccctc acccagctgt tcgtgaagac gggcgtacac cgcctccggt
    12841 gccgtgtcat aaccggcaat acgtatctgg taccagcctt cgttcatctg acggcgaaag
    12901 cccggcatct gcatcgacag ggcgcgaatg gcttccgctg ccgtgttcac atacaggctg
    12961 aggcggcggc caaatcgttg taaatccccg tgaaggcaga tgcgtgccag tggcggtgac
    13021 gccagactga atgcgttcgt cgttgccatt tttcggaata cctctcccgt ttactcagtt
    13081 gttcaggcag atggtgaagc agctcaccgt tgccgcagta aatggcggca tggttcggta
    13141 ccgaagcacc aaagcagcac agcagaatat cgcccgcctg tgcagaggac aggggcaccc
    13201 ggtaaaagcc ggtgaccgcc atattgtcca ggtaaaggtt ctgaccgttg cgccaccagt
    13261 catcctcgcg atgaaaatcc ggcatttcag tccccgccag atgataagca tcccggaaca
    13321 gcgtgtaaca gtccgtcacc ccgtgctcaa agcgccgtcc tgtcagatgt ggcacacagc
    13381 ggaatttgtg aatgtcaccc cggcagacca accaccaggg cagtgcgctt tttatctgca
    13441 accgccggtc agcctcgctc agccagggca gcccaccggg atgactgtgg accagtgcca
    13501 caatctcccc ctgcatctct gcccgcagcc agtcttccgg tgcgatacga aaatacgcct
    13561 ccggctctgc ggaaatattc acacaaggga tataccgctc cccctccggc gtgcttatca
    13621 cgaagccgca cgactccgca ggcgcacacc gccgggcatg tgccagaatc gctgattcag
    13681 tctgtgtcat aaaccgggat ttactgcgaa agtttattaa tggaaaggaa accgccaaaa
    13741 ttgccgacat tcctgcgcag ttcacacccg cgcatgcact tgctgcatct gtccttacgg
    13801 atatccgtgg tggggttgtc gaactcatcc gccaccgcag gaccgttata cccgcattca
    13861 tctccccggt aatcccacat acaggtgttc gccagcatga tgcgaccggg aaacagcgcc
    13921 ccgtccgtct cggtcggtgt agccagcaca aacgaggccg tcatggctgt cagctgcgac
    13981 atctgctcca ccacccagcg gtcactcagc tcctgctccg ggtccgcctc cggattgccc
    14041 gcaacgaaat tcaccgcatc cagaaaacgg gcatacaccc ggcggcggac caccgtggcc
    14101 cccaccagac tctgcaggtc ttccgccatc ccggtgacca gaccgaacag attggacacc
    14161 gtcagcgacg gtctggcact gctgccccgg ccgttcatct caaagccgct gccgtcaatc
    14221 gggtatgcct catacttacg cccctgccag gtgaccggct cccctttttc attcagctca
    14281 ttacagaaaa aataccgctc accaccctgt accgtcaggt cgatttccca gagtaccacc
    14341 cgcggtgact gctctgactt aaccgactcg ttcagacttt cttcgcgaat atcctgcatc
    14401 agttcaccac ctgcttaaac tccgcgctga actcaacgcg caacatcccg acccgcgcag
    14461 accacccggc acaggtcacc tttatctgcc ggtatgcata gggtggcttc cacaaaaatg
    14521 ccttccagcc accgtgctct gccaggaacg cttccagatg ccgggcctcc tcccgggtca
    14581 cggaaagcgt caccctgtat gttttcaggt cagcattcag ccctgccgcc atacgctgtg
    14641 agtacccgtc accaaaacgc acttcacgca ccgatggctg cgagttcacc tccatatccg
    14701 gcttcacttt ccagcgaaag gttttcatcc accgctccct gataacatac cgccatcacg
    14761 caactgcagc cggagttcat cctgtgcccc cttgcgggcc atctcataca ccgctttcat
    14821 cagctgcggc cctgcccgcc cgttggggcc gtcgttctga atcaccacgt gattgttctg
    14881 attaaaatta atgccttccg cccgccgcat ctgcgccgga cttccggcac cgccgacata
    14941 accaccttcc gcatagcccc gcatcaggcg gtacagattg ccgacaccaa tccggctggt
    15001 cgcctccttc gtgaagacaa actccccgcg atgaacaatc cccgcaggtt catatttacc
    15061 ccccgtcccc gtaaatcccc cggtcgcgaa atggaagttc gccgccgcag cctgaatggc
    15121 cgtccccgtg gaggcagacg caccaccacc gaaagcaccg ccaatggcgc tgccgatact
    15181 cccgactatc cccaccatcg cctgcttcag aaaaatctct gtcagcatgg acagcacaga
    15241 acgggtgaaa ccacgccagt tctgttcgct gccgatcagc atcgctgcca tattctgtgc
    15301 aataccgtca aaggtctgcg tggccgcgtt tttaacctgc gaaaaactgt ccgtcgcact
    15361 ttccgcccac tcgccccagc cggacttcat cccggccatc cagcttccac gaagctgctc
    15421 ctccgcagac caggtgttct tcagtgcaga tgtggccttc gccagcgcag ccggattatc
    15481 accgtacacc tcacgaaggc gctgctcttc cgactcccgc tgcgcctgac ggtcggtgag
    15541 tccgcgggct tttgcgctga ttgccgcctg cttcgcgctc tgctgctgtt caaaccgcgc
    15601 cgcctgctgt gccagctcat tcagccgttt ctggtgttca atcttgtcgc ccagctcagc
    15661 cagctggcgt ttgtactcca gcgtttcttt ctcatgggcc agcagggatt tttcctgctc
    15721 agataactgc cgtttcgtgg cggcctcttt caggaccaca tactgatttt ccgctttcca
    15781 taaatcacgg cgctgctggc tgattttttc attcgcaccg ctgtgcttct ccagcgtcct
    15841 gagctcagtt tcaagcgcca gcagggctgc atgcgcctgg tcttcctggc gctcaccggc
    15901 agacaccttc acaccggacg gctttttctg cgtcgactca taatcctttt ttgccgacgc
    15961 catcagcgtg ttgtaatccg cctgcaggat tttcccgtct ttcagggcct tattcagctc
    16021 ttcctgccgg gcggtatatt tctccagtgg cgtcagcagg cgctcatacg ccttctgcgc
    16081 ctctccggta tacttcagct gtgacgactc acgctcagcc ctgtcccttg ccgccagttc
    16141 accggctttt tccatatccg actgcagcgt tgccgctgcc agacccagac gggcattttc
    16201 ccggtcatcc catgcgccct gaaggttggc ccggaaagag gaggttttac cgcggcgctg
    16261 gctccgactc tggtaccact gccatttttt atccgcctca tcaaatgcct tctgcgcact
    16321 ggcgagcata tccgctgagg attccggacg accgatatcc agaatggcat cccacatcga
    16381 tttgaatgcc ttccctgttt tatccgccca ggtctccagt gttcccatgt tttctttcag
    16441 gcgacgggtc tgctcatcaa agcctttcgt ggcgatatcg ttcgccgcct gcaatgcccc
    16501 ggcctcgtct ccggaacgct gcagctgtgc aacatacgca atctgctctg ccgtcacgtt
    16561 acggaactgg cgcgccatcg ccatcagtcc cgacgtcggg tcagtggtca gcttcccgaa
    16621 ggcttcagcg actttatcca cctccacacc ggatgcagac gcaaaacgcg cgacactctg
    16681 gttgatggca tcaaactgtt caccaccacg cacaccggca ttcaccaggg ctgccagtga
    16741 ctctctcgcc tggttaaacg tcagccctgc tgcctgcccg gctcttgaga gagtcagcat
    16801 acgatcggca gtcagtccgg actgattacc ggaaagaacc agggttttat taaacgctga
    16861 aagcgtggaa tctccctggt accaggcgta caccagtgca cctgtcgcca ccgccagcga
    16921 ggtgaccccg accatcggca gggtgatcgc atcggcaagc cccctgaaca tggggatcat
    16981 cccgccgaag gagtccttca cctgaccgcc ctgttgcagc aggatcagcc agggattctg
    17041 accaccggca agctgcgtgg cgatatccgt aaactgtgcg ggcagggttc gcatggccgc
    17101 tttatactgc ccgacggaaa tcccggcttt ttgtgcagcc agcgcctggc ggctcaggcc
    17161 ctgttcaaca gcactggcgg tttttctggc gtcggtatcc agacctgaaa aatgacgcct
    17221 tacccggctc atctgctcat cgaaacggac agcatccaga ctcaggtcaa taacaagatc
    17281 accaaccggc tgggacatat ctcacacctc ccggaatccc cgctgaagcc atcattaatg
    17341 cggcatcatc caccatgaca tccgccacat ccgcagacga taaaatatcg cgccctccgt
    17401 ccccaccgaa ccggacgcct ccggcaagtc ctgccgcttt ctgcatcagc attttgtcct
    17461 catccggcct ctccacctgc tcttcctcat gccgggggac aagcagactg aaatcagagg
    17521 gatgcatatc cggatcgcaa aaaaacaggc tgagtacagc gtacgtcagc ccggaaaaat
    17581 gcatatccag ctgggtatcc tgaaaataat gcgtgcggta aaaacggtgc cagtcggcat
    17641 attcggtgga tgtcatcccg gcaagcatgg cgcgccagtc gggtctcccc atctcacgcg
    17701 ccagtctgag ggcaaagttc agctcgccgt cgaagacttt cccgcagaaa aatcatcatc
    17761 agtcagcgtg ttatttttcg ccacttcagt aatatcagta tccggacgaa cagcttcgat
    17821 catcccggac aggcacaaca ccacgtcttc cgcccgggca atggcatcgg caggccaggt
    17881 ggtgagcact tcctgctcta tcttcatcac ggcctcattc attgacggtg actgcgtttt
    17941 ctgtggatgg ttatgccaca gggacatcgc caccagaaac gcgccggttc tgacgagatc
    18001 ttccacgctt acctgcaggt tgccgctgga ttctgcctgt tctgcacgcc gtttcaggag
    18061 ggcaagatgc tcgatacgct gcagcgcaga caattcggaa agcgtgacag acacaccgtt
    18121 atattcaaat tgttctgttt tcagaaacat gtattacctc cgtttaccct gcagcgcccg
    18181 cttcagtaac ggtgacttca gccactgcgg cgaactgacc atttccgctc accacaggga
    18241 tctgcacctt acctgtcgcc acgccgttta ccgtaattgt catatctttc acactaatgg
    18301 tggctttcga cggatcggcg gaaaccgctc tgaacgtctt gtcggttgca ctttccggct
    18361 caaaagaaac cgtcagggtg gttgttttcc cttttgccac cgtaccggat gtcggcgtca
    18421 ccttaatcgc actgaccggc gtaattttgc tgcgttcttc cgctacagaa ggtttaccca
    18481 cgttagtgac tttcaccgtg cgggtgatca cttctttcgc cgtcacggcc ttaccgatac
    18541 tgctgaccca gccacgaaac acatccaccg tgccattcgg aaaacggatt ttataggccc
    18601 ggacatcgcc gctttcaaac cagcctataa gccctttctg gccttcctct cccggtttcc
    18661 aggccagcgt aaaactggta tcacctgcag atttctgccc ctgcccggtc gcgctccagt
    18721 ccgcgtcttc atcatccagg tagttatcat cataggattc agccgtcatc tcgcccggcg
    18781 tcagatcctt caccttagcc agtcgctgcc agtcatcgtc tgacaacggg tttgcataag
    18841 catcagcctt gccgttgtaa acccacagag tggtaccggc accttttacc ggctccaggg
    18901 gatttggtgt tgccatatcg tcctcacatc tcgtatgtaa tggaataagt cagatccgca
    18961 gagctccata acgccatatc gtcatcacga cgatactcat agccctgcgt aaccatcgtg
    19021 gtaatcagtc ctgccagtgc agggatcgca gtcatcgccg gataaatccg gctttccatc
    19081 cactgatcga gctccgaatc aggtacctgt gccggtaaaa acacctcaat atgcagtgtg
    19141 gcccgccagg tatctgcatc cagctcttca ccggtatact ctgcatccgt cagataaacc
    19201 gcgatcgcag gaaaatcctc ttcgtcaaaa acaacggggc gaccatcaaa cagcgtcgcc
    19261 ccgtgttcat gctgctcgag tgcatccagc actgcggcac gaatgtcagt gtgtttcatc
    19321 gttttatcgc aatcctcagt tgttgtttca gcgcgtatgc cagttcttta ggcaggcgtt
    19381 cacgccggat acggtcaaca ttctcatcaa atgcctgttt cagtggggcc gccatcggga
    19441 ttttcaccac ctgaatggga aggcgattac gctttttcct tcccttgtcg tcattgccct
    19501 cctcatatct ggcctgggga agacgttgca taacatgcca gcgcccatta tttaatcgct
    19561 ggataaatgc ccgctgataa cgatgctgac cggctttgag tatgctgttc ggacgacgcc
    19621 ccagcattct gatccccagc ttaatcacag ggagatcacc gcggttaacg ataattctgg
    19681 cattcggatt tctgaccgtc gcccgtttca gtctggaccg ttcctttacc agtttccgtc
    19741 tcaccctggt ttcccgggca acctgtgacg aagactgatt aatcgccgtt gtggccacgc
    19801 ggttaatggt cattgcagaa gcagccggaa tggcgttttt acgaacccgg ctcagattgt
    19861 caatcgcctg atcaagccct tttatcgcca taattcaccc tgcgtttatc gtcgccggtt
    19921 aactgcgggt ggttgcccac ggttgagcca gagataacag ctgcccccgt catccgga
//
LOCUS       DAKISA010000085        19799 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.085, whole genome shotgun sequence.
ACCESSION   DAKISA010000085 DAKISA010000000
VERSION     DAKISA010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19799
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.085"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..453)
                     /gene="spy"
                     /locus_tag="OVW45_003048"
     CDS             complement(<1..453)
                     /gene="spy"
                     /locus_tag="OVW45_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCV5527438.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPA"
     gene            complement(783..1751)
                     /gene="astE"
                     /locus_tag="OVW45_003049"
     CDS             complement(783..1751)
                     /gene="astE"
                     /locus_tag="OVW45_003049"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCV5527439.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGDPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(1744..3087)
                     /gene="astB"
                     /locus_tag="OVW45_003050"
     CDS             complement(1744..3087)
                     /gene="astB"
                     /locus_tag="OVW45_003050"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HCV5527440.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(3084..4562)
                     /gene="astD"
                     /locus_tag="OVW45_003051"
     CDS             complement(3084..4562)
                     /gene="astD"
                     /locus_tag="OVW45_003051"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HCV5527441.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDSAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARVGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(4559..5593)
                     /gene="astA"
                     /locus_tag="OVW45_003052"
     CDS             complement(4559..5593)
                     /gene="astA"
                     /locus_tag="OVW45_003052"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCV5527442.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRVDFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(5590..6810)
                     /gene="astC"
                     /locus_tag="OVW45_003053"
     CDS             complement(5590..6810)
                     /gene="astC"
                     /locus_tag="OVW45_003053"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HCV5527443.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLTTEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            7256..8062
                     /gene="xthA"
                     /locus_tag="OVW45_003054"
     CDS             7256..8062
                     /gene="xthA"
                     /locus_tag="OVW45_003054"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCV5527444.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            8229..8939
                     /locus_tag="OVW45_003055"
     CDS             8229..8939
                     /locus_tag="OVW45_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCV5527445.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFVLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            8944..9621
                     /gene="ydjY"
                     /locus_tag="OVW45_003056"
     CDS             8944..9621
                     /gene="ydjY"
                     /locus_tag="OVW45_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCV5527446.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFIAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            9636..10343
                     /locus_tag="OVW45_003057"
     CDS             9636..10343
                     /locus_tag="OVW45_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCV5527447.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAADLTSIRFRSFFIATGLGQLPTTIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            10343..10891
                     /locus_tag="OVW45_003058"
     CDS             10343..10891
                     /locus_tag="OVW45_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCV5527448.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            10901..12067
                     /locus_tag="OVW45_003059"
     CDS             10901..12067
                     /locus_tag="OVW45_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCV5527449.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            12040..13576
                     /locus_tag="OVW45_003060"
                     /pseudo
     CDS             12040..13576
                     /locus_tag="OVW45_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            13576..14229
                     /locus_tag="OVW45_003061"
     CDS             13576..14229
                     /locus_tag="OVW45_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCV5527450.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALL
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            14296..15603
                     /gene="ynjE"
                     /locus_tag="OVW45_003062"
     CDS             14296..15603
                     /gene="ynjE"
                     /locus_tag="OVW45_003062"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HCV5527451.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(15612..16232)
                     /locus_tag="OVW45_003063"
     CDS             complement(15612..16232)
                     /locus_tag="OVW45_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCV5527452.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            16319..16726
                     /gene="nudG"
                     /locus_tag="OVW45_003064"
     CDS             16319..16726
                     /gene="nudG"
                     /locus_tag="OVW45_003064"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCV5527453.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFADGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            complement(16692..16964)
                     /locus_tag="OVW45_003065"
     CDS             complement(16692..16964)
                     /locus_tag="OVW45_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCV5527454.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            17200..18543
                     /gene="gdhA"
                     /locus_tag="OVW45_003066"
     CDS             17200..18543
                     /gene="gdhA"
                     /locus_tag="OVW45_003066"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCV5527455.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(18660..19700)
                     /locus_tag="OVW45_003067"
     CDS             complement(18660..19700)
                     /locus_tag="OVW45_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCV5527456.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMTSISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
BASE COUNT         4700 a         5276 c         5083 g         4740 t
ORIGIN      
        1 cgctggacgt tctgtcagac gcttctcaaa attagcatta aattgctttt tctgttccgg
       61 cgtcaggatg ttgtaaattt tgttctgggt ttccatgtgc gccagcatgt tagctttgcg
      121 ctgttcttcc atttttgcga tctgcgcttc agcttttgct ttatcgaagg tatcgctggc
      181 aatgatgtca tgcattgcgc ggcgttcttc cagcggcgga cgtttcatct ggtcacgctg
      241 gcctttcatg atttcgcgga tctgctgttt ctgcgcgtcg gtcaggttca ggtctttgaa
      301 catcatgtcc tgatgcggac cgaacttgcc tttgtggtgc atcatcggct tcgcgtcagc
      361 cggtgctgcg gtagtggtgt ctgcggcatg ggccaggtta gccgcgccaa gagccagggt
      421 agaggcaaca aacagtgcag ttaatttacg catattctat atccttcctt tcagttattt
      481 attacggctt tcttaagtag cgtgccgtgt tgacgagatt aactttactg actttagcgt
      541 caattaatca gagcaacggt aaaacaatga aagtgtaaaa aacacttttg cgccaattat
      601 ggagaaaaaa agaaaatttg atggagagtg atgagagaat attacaacac aatgattttg
      661 cagagattat gaagaactat accggatgac tggtgataaa taaagcaaat aaccaggatt
      721 aatctgtatt aatttataag aaagcaactt aatacccgca gaatgatttc tgcgggtaag
      781 tattagctta ttttttcgag cattaatccc gcgcgtaatc ccaacgctac caacggatta
      841 gggaataaca catactctac atcatgggtt acggtaaaac gttcctctcc gtcctgcgcc
      901 agcaatgttc ctttctcaaa cggcataaaa ttcagcgtgt cacttgccat atgcatttcg
      961 aaggacggcg agtggcgagt aatttgcgaa accacccgat aacggagcgg cggtgttctc
     1021 acgataccga cactctcacc agatagcagc gcagcaattg cgctggcagt tactgcaaac
     1081 tggcgaagat cgttttgccc aaagggcaac gctttgccaa gttccagcgt acaggccagc
     1141 gcgccaaaat gtctggcgct gaaatgggta aacgtaccac caggctcctg atggaacacc
     1201 agcgcctcca gccccgccgc acccagccac gtcagaaatt tctcgtccca gggaatgtcg
     1261 cgttgcggta atacaccgaa ctgaggatgc aaggagccac gaattgcggt atgtagatca
     1321 aggtgccaac gcacagattc tttgccctgg tcataaaaat cttccaggca ctgttccagt
     1381 tcgcgcgccc gacaggtttc tccgctttca gcaaatagct gccaacgacc gccaaacatt
     1441 cggttcatat cgctatggca ataacgtttc ccttgcttca gcgcaggagg atccccgagg
     1501 atcaccagca accgccaacg taacgggatc tcgccgtgag atatcgcgcc aagcaacgcg
     1561 tccagcattt ccacaggtgc cgtctcatta ccgtggatcc ccgcagaaat caccagcgcg
     1621 ccttgcggtg gcgttaatgg cgtcagttcc agcacaccat cgcccagcca gcgccagcga
     1681 acgccgttga tttctcgctc ggtgataacc ggttttttac ccgttaaggt cagagcaaga
     1741 aaattatcca ttgccccctc cctcgcgctg gaacggataa accgaaccga gattcagtaa
     1801 ttggctcaat acatccagtg cttcccgccc ttcgcgcagc aattgcgggt cggccagatc
     1861 ggcagcagta aggcgatcgc ggtagtaacg atccacccag tcattgagcg cattaaacag
     1921 cgtatcgttc atcatcaccg ccggattcac cgcccggcgt tcttcttctg tcaatactac
     1981 ccgcaaccgc aggcacgccg ggccgccgcc attcgccatg ctttcacgta aatcaaagac
     2041 ttttagttcg ctaatcgggt tgtcagcggc aaggagttca ttgagataac cccatactcc
     2101 ggcgtgttcc cgacactcct gaggcagcac caacatcatg gaaccatcat cgcggctcaa
     2161 cagttggctg ttaaacagat aggtagacac cgcatccgac acggaaacct gagttgccgg
     2221 aacttctatc gccataaaac cgttcacccg cgcccgcagg tttgccagta actgtgactg
     2281 gcgagcgaac gcctgttggt ggcaaaacag cacctggcgg ttacttacgg caatcacgtc
     2341 attatgaaaa acaccctggt cgataacgtc cgggttttgc tgggcgaaaa tcacctgttg
     2401 gggattcacc tgattaagcc ttgccaccgc ctcgctggct tcgcgagtct gtcgcgccgg
     2461 ataacgggaa ggccgggtat cattgccttc ttctcgcccg tagacaaaaa gttgcatacc
     2521 cggctcaccg taatgaccgc cgagacgatt gtggtttgcc gccccctcat caccgagcaa
     2581 cgctacctgc ggcaacgccg aatggacgct aaatttctct tcgtcgttaa aaatagcttt
     2641 taacagcgat tcagtgacgg gcgcttccag cgaacggtga aatttattgt tcaggttggc
     2701 aacggtgaga tgcactttgc catccagcgt atcggcagat ggcgcgatcg ttgccgcatt
     2761 ggctacccac attggcgaag cggagctgac gctggaaagc cagtgcggtg cctggcgtgc
     2821 aactttttcc agtacctgct catcgctacc actgaatccc aactgacgca gcaccggaat
     2881 aaacggacgc tcgtgcggcg ggatcacggc ctgggggaat cccgcatcgg caagggtttt
     2941 cattttcagt aagccctgtt tcgccgccag tcgcgggtta gacacctgaa aacggtgacg
     3001 ggtagaggct tcattaccaa acgacaggcc agcgtaatga tgcgtcagcc ctaccagccc
     3061 gtcgaaatta acttcccagg cgttcatcgc accacctcat cggaaaaatc cagcccgggg
     3121 ttaagcgttg cgggcaaagt taacgagtcc gactccaggc tcgccatagg ccatgcgcag
     3181 taatctgcgg cataccaggc gctggggcga tggttaccgg atgcaccaat gccgccgaat
     3241 ggcgcggtac tggcagcacc agtaagcggt ttgttccagt taacaatccc cacccgcgcc
     3301 tccagcaaca gttgatcgaa tttttcccgc tcgggggaaa ccagaccgca agagagtccg
     3361 aagcgagtgt tattcgccat tagaatcgct tcctcgaaag aatcataacg ccagacgcgc
     3421 aataacggtc cgaacacctc ttcatctggt acgccagcaa cgcctgtcat ttcaatgatc
     3481 cccggcgtca gcaacgatgt ctctgattgt aataagcgcg gcgcaagcag ggttcgtccg
     3541 cccatcgctt ccagttgctg ccaggcagta accacctgct gtgcggcctg ttcagaaatc
     3601 agcccgccaa taaacggctg cggttcgtca tcccagttgc ccggcgttaa tcgctggctg
     3661 acggcaacca gacgagcaag aaacgcatcg ccctgtgctc cgcttttgag caataaacgg
     3721 cgggcgcagg tgcagcgttg cccggctgtg acaaacgccg actgaatggt cagatggaca
     3781 gccgagtcga tatccgccac ctcatcgata attagcggat tattaccgcc catctcaagg
     3841 gcgagaattt tctccggctg accggagagc tggcgatgca gctggtagcc ggtattggcg
     3901 ctaccggtaa acagcaaacc gtcgagatcc tccagcgcac tcagcgcctg acccgtttca
     3961 cgcccgccct gcaccaggtt cagcacgccc ggcggcaagc cagcctgctg ccataaacgc
     4021 attaccgctt cgccactcca cggtgtcagt tcgctgggtt taaagataat ggtgttacct
     4081 gccagcaatg ccggaacgat atgtccgttc ggcaaatgac cagggaaatt atacggccca
     4141 aacaccgcca gcacgccgtg cgggcgatgt cgcaggctcg ccgcaccgtc cggcatttca
     4201 ctacgctgct cgccggtacg aacgtgatac gccttaattg atatcgcgat tttattgatc
     4261 atcgccgtca cttcggttgc cgcttcccag cgcggcttac ccgtttctct ggcaataatc
     4321 gcggttaatt cggctttatt gctttccagc aatccggcaa agcgttcgac aacgacctga
     4381 cgttcagcca atgagagccg cgcccagcgc ggaaacgccg cacgggctgc ccgacaagcc
     4441 tgttcgacct gagcggcatc ggcatcattg ccttgccata acacctcgcc cgataccgga
     4501 ttacgcttca cacgcgatgc gccctggccc gttatccagt caccgttaat ccataaagtc
     4561 atgctgtttt ctcctctgcg cacaggcgca ccagacgaac gcgatcccct gcgtggcatt
     4621 tgagggcatc cagttgtgcg gcggttaaaa tcaaacgctc ggttgccgga tcggtacgcg
     4681 ccagcaccac gcggaaatgg tgataatttt cattggcgac caggcaggcc ggaaaatcgc
     4741 cctgcgcagg ctgcccttct gccacttcca ccagccgact tttacggatg gcgcgcacgc
     4801 ggtcgatgtc acactcaagc gttggcccgc cgtcgaagat gtcgatatag ttgcggtaac
     4861 gaaaaccttc tttctccagc accgcgcggg caggcgcggt ttgcggatgt acctgaccga
     4921 tgacgtcctg ggcttcctgg gataaaaagt gggtatagat cggatgtttc ggcatcagtt
     4981 ctgcaataaa tgccttttgc ccggtgccgc agagaaaatc gacgcggcta aaatccatcg
     5041 aaaagaagcg tttaccgagg ctttgccaga acggtgaata gccgtgttcg tcaatcaccc
     5101 cgcgcatttc agcaaccact ttgtcattaa acttgtcgcg aaaagccgcc ataaacataa
     5161 agcgcgattt cgacagcaaa tagccgttgc cctctttgcg ccagtccggg tcgagaaaca
     5221 gcgtgcacag ctcgctgctg ccggtgtgat cgttactaag aaacagcgtc ggcaatgcgt
     5281 tatagacatt cagctctttt gaggcgtgaa ccaacgtgcc gacccgatag ttgtaccagg
     5341 gatcgttcag cccaaccgcc acctcaatgg cgcaaatccc cgccacggtg cctgtctcgc
     5401 tatcttccag cacgaacaca tagccctgct cgcttttggg cagttcgcct tgccaggttt
     5461 tgattgccct ttcgatacgc gcggaaagcg tggcttcatt ggcgggaagc gacgtcaggc
     5521 cgccgcccgt tttgctggca agctgcatca gcgccgagac atctgagcgc tcaacgggac
     5581 ggatgaccat catgatgaac ctcggctaac aaagtgttcg caagcagctg caaagcgatc
     5641 cagtccggtc gtcacctctt cttcgctgac attgagcgca ggcgcaaaac gcaccacgtt
     5701 gccacccgca atcagtacca taacgcctgc tttcgccgcc tcctgagaga tctgtttcgc
     5761 ttgtccggcg taatcggcat tcagtacaca gccaatcaac aaacctaagc cgcgaacttc
     5821 actgaacaaa ccgtagcggt gattaatggt attaagacgc tcaacaaacc agtcgtgacg
     5881 ctgtttaacg ccattaagca tctctggtgt gttgatgagc tccagcactt tgcccgccac
     5941 cgccgaggcc agcggattgc cgccataggt ggtgccatga gtgccaacgg tcatcacgct
     6001 ggcgcactct tcggttgtta acaacgcacc gaccgggaaa ccgccgccca gcgctttggc
     6061 ggtagttaac agatcaggtg tcacgccgta gtgcatatag gcatacagtt ccccggtgcg
     6121 cccgacgccg gtttgtactt catcaaaaat caacagcgca ttgtggcggt cacacaattc
     6181 acgcagacct tgtaaaaacg cgttgctggc tggcaccaca ccgccttccc cctggatggg
     6241 ttcgacaatc accgcacagg tggcgtcgtc aatcagcgcg ctggcagagt taatatcgtt
     6301 atatgcagca tgacggatat ctggtggcag tggcgcaaaa tcctgtgaat aggctggctg
     6361 cccacccgca ctgacagtaa acagcgtgcg accatgaaac gcatttttaa acgccacgat
     6421 accgctctta tggctgccgt agcggtcgtg agcgaattta cgcgccagtt ttagcgccgc
     6481 ttcgttggct tccgcaccgg agttacaaaa gaagacgcga tcggcaaacg tggcgtcgat
     6541 caattttttc gccagtcgca gtaccggctc gttggtgtaa ccgttgccgg tatgccagaa
     6601 cttactcgcc tgttcgttca gcgcttcacg cagttccgga tgcgcatggc ccagcgcgtt
     6661 caccgcaatg ccacccgcga agtcgatata ctctttcccc tgctgatccc acaagcgcga
     6721 accttcgcca cgtaccggta taaagggtgc cggagcgtaa acaggtatca tccattcatc
     6781 aaagttttca cgcgtaattg gctgagacat agcgacctct acagtaaata attcgttatc
     6841 tatatgttaa caataagtaa tgtttgcgct gtaaatgtag attgcagggt tcgtgccagc
     6901 cagtgataaa agtgcataaa cggcggaggc taactggaaa tcaaggagtt ataaccaaac
     6961 catatgcatt taaagtgcat ataaagtgaa tacgtttgcg atgtgggtga ataaaaagaa
     7021 taaaaaacgc aatgttatgc agaagtaaaa tataattctg gaattgtgat cattgacgaa
     7081 atttactgga aattactgcg ccattctgac gcagcgcgca ccaaaagcgg gcattttttg
     7141 cgccatcgtt gacatcatta acaaccatcg atcaaatcac ttaacaacag gcggtaagca
     7201 acgcgaaatt ctgctaccat ccacgcactc tttatctgaa taaatggcag cgactatgaa
     7261 atttgtctct tttaatatca acggcctgcg cgccagacct caccagcttg aagccatcgt
     7321 cgaaaagcac caaccggatg tgattggcct gcaggagaca aaagttcatg acgatatgtt
     7381 tccgctcgaa gaggtggcga agctcggcta caacgtgttt tatcacgggc agaaaggcca
     7441 ttatggcgtg gcgctgctga ccaaagagac gccgattgcc gtgcgtcgcg gctttcccgg
     7501 tgacgacgaa gaggcgcagc ggcggattat tatggcggaa atcccctcac tgctgggtaa
     7561 tgtcaccgtg atcaacggtt acttcccgca gggtgaaagc cgcgaccatc cgataaaatt
     7621 cccggcaaaa gcgcagtttt atcagaatct gcaaaactac ctggaaaccg aactcaaacg
     7681 tgataatccg gtactgatta tgggcgatat gaatatcagc cctacagatc tggatatcgg
     7741 cattggcgaa gaaaaccgta agcgctggct gcgtaccggt aaatgctctt tcctgccgga
     7801 agagcgcgaa tggatggaca ggctgatgag ctgggggttg gtcgatacct tccgccatgc
     7861 taatccgcaa acagcagatc gtttctcatg gtttgattac cgctcaaaag gttttgacga
     7921 taaccgtggt ctgcgcatcg acctgctgct cgccagccaa ccgctggcag aatgttgcgt
     7981 agaaaccggc atcgactatg aaattcgcag catggaaaaa ccgtccgatc acgcccccgt
     8041 ctgggcgacc ttccgccgct aatttagcag ctctcctggc tcaaactggg tcaggagaat
     8101 taaccttgag aaaaatcaac aaactgtcag taatgatttg ttgcctgccg tcctttgtta
     8161 taccgtcact gcgtttttag ttgtctgacc acttctctat tatcaagttt gatataggaa
     8221 actccacgat gaatgctgag cgtaaatttc ttttcgcctg tcttattttt gcgctggtca
     8281 tttacgctat ccacgctttc ggtttattcg atctgctcac cgatttaccc cacttacaga
     8341 cactcattcg ccagagcgga ttgttcggtt atagcctcta tattctgtta ttcatcattg
     8401 ccaccctctt tgtgttacca ggaagcatac tggtgatcgc tggaggaata gtttttggcc
     8461 cgctcttagg gacactactc tcattaattg ccgccacgct ggcctcctcg tgctcattcc
     8521 tgctggcgcg gtggctggga cgtgatttac tgctgaaata cgtgggccat agccatacct
     8581 tccaggccat tgaaaaaggc attgcgcgta acggtattga ttttcttatt ctgacccgct
     8641 taatcccgtt gtttccttac aatattcaaa attacgctta cggattaacc accatcgcct
     8701 tctggcctta tacccttatt tcggcactca cgaccctgcc cggtattgtt atttataccg
     8761 tgatggcaag cgatctcgcc aatgaaggca ttacgctgcg ctttatttta caactctgtc
     8821 tggcgggcct ggcgctgttt attctcgtcc agctcgcaaa actctacgcc cgacacaaac
     8881 atgtggatct gtctgcttcg cgccgcagcc cgcttactca cccaaaaaat gaaggataga
     8941 acgatgtcgc aacattattc agtgtcatgg aaaaaaggac tggctgcact ctgcttactg
     9001 gctgttgccg ggcttagcgg ctgcgatcaa aaagagaatg cggcggcaaa agtggaatac
     9061 gacggacttt cgaacagcca accgctgcgc gtcgatgcca ataaccatac ggtaaccatg
     9121 ttggtgcaaa ttaatggtcg tttcctcacc gacgacactc gtcacggtat tgtgtttaaa
     9181 gatggctcca acggacataa atcgctgttt atagcttatg cgaccccgaa agcattttat
     9241 gaagccctga aagaggcagg tggtacgccg ggcgaaaaca tgacgatgga taataaagaa
     9301 acgactcatg tcacaggcag caaactggat atttcggtca actggcaagg ggcggcaaaa
     9361 gcgtattcct tcgatgaagt gattgttgac agtaatggca agaaactgga catgcgcttt
     9421 ggcggtaatt taacggcagc agaagagaag aaaacgggtt gcctggtgtg tctggatagc
     9481 tgcccggtcg gcatcgtcag caatgcaaca tacacttatg gtgcggtgga aaaacgcggc
     9541 gaagtgaaat tcaaaggcaa tgcctcagtt ctcccggcgg ataacacgct ggcaacggtt
     9601 acctttaaaa tcaccgaata aagccaggat aaaggatgat gaagatgcaa tcgcgtaaaa
     9661 tctggtacta ccgtataacc ctcatcatcc tgttgttcgc tatgctgctc gcatgggcgc
     9721 tgcttcccgg cgtccatgag tttatcaatc gcagcgttgc ggcgtttgcc gccgtggacc
     9781 aacagggtat agaacgcttt attcagtctt acggcgcact ggcggcagtt gtctcgttct
     9841 tgttgatgat tttacaggcc attgctgcac cgctacctgc gtttttgatc acctttgcca
     9901 atgcgtcgct gtttggcgcg ttctggggcg gcttgctgtc gtggaccagt tcgatggccg
     9961 gcgcggcgct gtgctttttt atcgccagag tgatgggccg cgaagtggtg gaaaaattaa
    10021 ccggcaaaac cgtgcttgac agtatggacg gctttttcac tcgctacggc aaacacacca
    10081 tcctggtctg tcggttattg ccttttgtcc ctttcgatcc aatcagctat gctgccgatc
    10141 tgacttcaat acgttttcgc tcgtttttta tcgccaccgg gcttggtcaa ttaccgacga
    10201 ctattgttta ttcctgggcg ggcagcatgt taacaggcgg tactttctgg tttgtcaccg
    10261 gactgtttat tctgtttgcc ctgaccgtgg tgatttttat ggcgaagaaa atatggcttg
    10321 agcgccagaa gaggaatgac tgatgggttt accgccgctt agcaaaattc ctttcatttt
    10381 acgtccgcag gcgtggctgc atcgtcgcca ttacggcgag gtgctaagcc cgattcgctg
    10441 gtgggggcgg atcccgttta tcttttattt ggtgtcaatg tttgttgggt ggctggagcg
    10501 caaacgctca ccgctcgatc cggtagtgcg atcgcttgtc agcgcgcgca ttgcgcaaat
    10561 gtgcctgtgt gagttttgtg tggatatcac cagtatgaaa gtcgccgagc gtaccggcag
    10621 cagcgataaa ctgctggcag tggctgactg gcggcaaagc ccgctcttta gcgatgaaga
    10681 acggctggcg ctggagtacg ccgaagccgc aagcgtcaca ccgccaacag tcgatgatgc
    10741 cctgcgtacc cgactggctg cgcattttga cgctcaggcg ctcaccgaac tgacggcatt
    10801 gatcggcctg caaaacctgt cggctcgttt taattctgcc atggacattc ccgctcaggg
    10861 gctgtgccgt attccggaaa aacgttctta aggagagaag atgcgccatt gtgggtggtt
    10921 gctgggattg ttatcgctgt tttctttggc aacacatgcc agtgactggc aggaaattaa
    10981 aaatgaggcc aaagggcaaa ccgtctggtt taacgcctgg ggcggcgata ccgcaattaa
    11041 ccgctatctc gactgggtta gcggcgagat gaaaacccat tacgctataa acctgaagat
    11101 tgttcgtctc gcggatgccg cagacgcggt gaagcgcatt cagaccgaag ctgctgccgg
    11161 tcgtaaaacg ggcggctcgg tggatctgct ctgggtgaac ggcgaaaact tccgcacctt
    11221 aaaagaggcc aatttattac aaacgggctg ggcggagact ctgcccaact ggcgctatgt
    11281 cgacacacag ctgccggtgc gggaagattt ttcagtgccg acacaaggtg cggaatcgcc
    11341 ctggggcggc gcacaactga cgtttatcgc ccgccgcgat gttacgccac agccaccaca
    11401 aacgccgcaa gccttactgg agtttgctaa agccaatccc ggcacggtta cttatccgcg
    11461 cccaccggac tttaccggca cggcgtttct tgaacagttg ctgattatgc tgacgccaga
    11521 tcccgccgca ttaaaagaag cgccggacga tgcgactttc gcccgtgtca ctgcgccctt
    11581 gtggcaatat cttgatgcac tgcatccgta tttgtggcgc gaaggaaagg atttcccgcc
    11641 ttcgcccgcg cggatggatg ctctgctgaa agccggcaca ttgcgcctgt cgctgacctt
    11701 taaccccgcg catgcgcagc aaaaaatcgc cagcggcgat ttgccggcaa gcagttacag
    11761 ttttggcttt cgcgagggga tgattggcaa cgtgcatttc gtcaccattc cagccaacgc
    11821 gaatgccagt gctgcggcga aggtagttgc caatttcctg ctctcacccg atgcgcaact
    11881 gcgtaaagca gatcccgttg tctggggcga tccttctgtt ctcgatccgc aaaaactgcc
    11941 tgacgggcag cgcgaaatat tgcaatcaag aatgccgcag gatctgccgc cggtactggc
    12001 tgaaccgcac gcaggatggg tgaatgcact ggaacaagaa tggttacgcc gttacggtac
    12061 gcattaatct ttttgctgtg ggcgatggtg gcggtgattt atgcaccgct gatcccggca
    12121 gctctcacgc tgatatcgcc tgcgttgtcg ttgacacact ggcaggcgtt atttgccgat
    12181 ccacagttac cgcatgcatt actggcaacg ctggtatcga caaccatcgc ggcggtcggg
    12241 gcattgttga ttgccctgct ggtgattgtg gcgctatggc ctgggccgaa atggcagcgt
    12301 atgtgcgccc gtctgccgtg gctgctcgcc attccccatg tggcttttgc caccagcgcc
    12361 ctgctgctct ttgctgacgg agggctgcta tatgactatt tcccgtattt cactccgcca
    12421 atggaccgat ttggcatcgg tctgggcctc acccttgcag tgaaagaaag cgcatttctg
    12481 ctgtggatct tagcggcagt attgagcgaa aaacggctgt tgcagcaggt cattgtgctg
    12541 gattcgctgg gctacagccg ctggcaatgc ctgaactggc tgctgttgcc ctccgtcgcg
    12601 ccttcgctgg caatggcgat gctggcgatt gttgcctggt cgctttcggt cgtggatgtg
    12661 gcgattattc ttgggccagg taatccaccg acgctggcag taattagctg gcagtggtta
    12721 actcagggcg acgcggatca acaaacgaaa ggcgcactcg ccagtctgct gctgatgctg
    12781 ttactcgccg cctacgtttt gctgagctat ctgctatggc gcagctggcg gcgcactatt
    12841 ccccgcgtag atggcattcg caatgcctgc cacaccttta ttaccaggca ctacgctggc
    12901 gagtttttta cccttaaccg gtgtgctgtg tgtggttctg ctggcgatcc tcgcggatca
    12961 gtcgacgatc aatagtgaag cactcatcaa cagtctgaca atggggctgg tagcaacatt
    13021 catcgctttg ctcctgttac tgctgtggct ggaatggggg ccacagcgtc gccagttgtg
    13081 gctatggttg cccattctat tgcccgatct gccactggtg gcgggccagt acacgctggc
    13141 gctatggctg aacctggatg gaagctggac ggcggtggtc tgggggcatc tgctgtgggt
    13201 aatgccgtgg atgctgttta tcctgcaacc cgcctggcag cgcattgatt cacggttaat
    13261 tttgattgcg caaacactgg gctggtcgcg ggccaaaatc ttcttttacg tgaaatgccc
    13321 actcatgttg cgccctgtgc tgattgcctt cgcggtggga ttttcagtca gtattgcgca
    13381 gtatatgccg acgctgtggc tgggcgcggg gcgttttccg acgctcacca ctgaggcggt
    13441 ggcattaagc agcggcggca gcaacggtat tctcgccgcc caggctttat ggcaactgtt
    13501 gttaccgctt attatttttg ccctgaccgc gttagtcgca aaatgggtag gttatgtcag
    13561 acaaggactc cgctaatgct ctgcgtgaaa aatgtttcgc tacgtttacc agaaagccgc
    13621 ttgctgacaa acgttaactt tacggttgat aaaggtgaca tcgtcacgtt aatggggccg
    13681 tctggctgtg gaaaatccac tctgttttca tggatgattg gtgcactggc tggacagttt
    13741 tcttgtacag gtgagctatg gctcaatgag caacggattg acatgctacc caccgcacag
    13801 cgtcagattg gcattctttt ccaggatgca ctgttatttg accagttcag cgtcgggcaa
    13861 aatttactgc tggcgctact gtcgacgctc aaggggactg ccaggcgcaa tgccgtgaaa
    13921 gacgcgcttg atcgagcggg actggctgaa acttatcacc aggaccctgc gacattatca
    13981 ggcggtcagc gagcgcgggt ggccctgctg cgcgcccttc tcgctcagcc aaaagcgtta
    14041 ctcctggatg agccattcag ccgtcttgat gtggctctgc gcgataattt tcgccagtgg
    14101 gtgttcagcg aagttcgcga actggcgatc cccgtcgttc aggtaacgca cgatctccag
    14161 gatgttcctg ctgatagttc tgttctggat atggcgcagt ggtcagaaaa ttacaacaaa
    14221 ctgcgataac gcaaagtttt tctcaatgcg tcagttcaga atggcgcact caaaactaca
    14281 atgtcgggat tttcgatgaa acgtgtttct caaatgaccg cgctggcaat ggctttaggg
    14341 ctggcttgcg cttcttcgtg ggccgctgaa ctggcgaagc ctcttacact tgaccagctt
    14401 caacaacaaa atggcaaagc gatagatact cgccccagcg cgttttataa cggctggcca
    14461 caaaccttaa atggcccttc tggtcatgaa cctgccgcct taaacctctc tgccagctgg
    14521 ctcgacaaaa tgagcaccga acagctcaac gcgtggatca agcaacataa cctgaaagcc
    14581 gatgccccgg tggcgctgta cggtaatgac aaagatgtcg acgccgtcaa aacgcgactg
    14641 caaaaagcag gttttacgca tatctccatt ctgagtgacg cgctaagcga accttcccgt
    14701 ctacaaaaac tgccgcactt tgagcagctg gtttatccgc aatggctgca cgacctgcaa
    14761 caaggtaaag aggttacggc gaaacctgcc ggtgactgga aagtcattga agcggcctgg
    14821 ggcgctccta agctttacct tatcagccat attcccggtg ctgactacat cgataccaac
    14881 gaagtggaaa gcgaaccgct gtggaacaaa gtttctgatg aacaactaaa agcgatgctg
    14941 gcaaaacacg gcattcgcca tgacaccacg gtcattctgt acgggcgtga cgtatacgct
    15001 ggagcgcgtg tggcgcaaat tatgctctac gcaggcgtga aagatgtgcg tctgctggat
    15061 ggcggctggc aaacctggtc cgacgcggga ctgcctgttg agcgaggaac gccaccgaaa
    15121 gtgaaagcgg aaccggattt cggcgtgaag atcccggcac aaccgcagtt gatgctcgat
    15181 atggaacagg cccgtggact gctgcatcgc caggatgcat cgctggtgag cattcgttcg
    15241 tggccagaat ttatcggtac gaccagcggt tacagctata ttaaaccaaa aggtgaaata
    15301 gccggagcac gttggggaca cgctggtagc gactcgacgc atatggaaga ttttcataac
    15361 ccggatggca ccatgcgcag cgccgatgat attaccgcta tgtggaaagc gtggaatatc
    15421 aaaccagagc agcaagtttc attctactgc ggcaccggct ggcgcgcgtc cgaaaccttt
    15481 atgtacgcac gcgccatggg ttggaagaat gtttccgtgt atgacggcgg ctggtacgaa
    15541 tggagcagcg atccaaaaaa tccggtagca accggtgaac gcggcccgga cagtagcaaa
    15601 taacattgca attactgacg ctggagcgac ttcagcgtca gatacccact ccacacccgc
    15661 gtaaaggtcg tcatccagca caacgcaccg aagatccacg caaaccacgc aaaccatgca
    15721 gggaataaac atcccagcac aaacagtaag atcgtttctg tgccttcggt taatcccccc
    15781 agatagtaaa acgacttatg tgcataaccg gggttatcaa tctgatgttt tgctgccagt
    15841 gcagcaaagg cgagaaaact gctgcccgtg ccgataaacg caaacaacaa ccagccgcct
    15901 gccagcgcat tttgctccgg tgcagcaaga ataaagccaa acggcaccag cgcgtaaaag
    15961 agaaaatcga gagaaatatc aagaaagccg cccgcgtcgg taagctctct gcgccgcgcc
    16021 agcgcaccgt ccagaccatc aagcagcctg ttcaacaaaa tgacgactag cgccgccaga
    16081 taccagccca gcgccagaaa cggcagagcc agcacgccga tggcaaatcc cactaatgtt
    16141 aaaccatctg gcgtaatgcc cggtttatca agaacccgca cgcactgatg caacaacggt
    16201 ttaatccggg gatgaagatg gcggtctagc acaggcactc cttaaatata aagcctttct
    16261 gattgagcaa cagtgcggat attatggcat ttttcgctta tctgcccgtg tgtaatttat
    16321 gaaaatgatt gaagttgttg ccgccatcat tgaacgtgat ggcaaaattt tactcgcgca
    16381 acgccccgcc cagagcgatc aggcgggatt atgggagttt gccgatggta aagtcgagct
    16441 ggatgaaagc cagcagcagg cactggtgcg tgagttaaac gaagaattgg gcatcgaagc
    16501 aactgtgggt gagtatgttg ccagccatca gcgagaagtt tcgggccgga ttatccatct
    16561 tcatgcctgg cacgtacccg acttccacgg gacgttacag gcacatgaac atcaggcgct
    16621 ggtctggtgc tcacctgaag aggcgctgca atatccgctg gcccctgctg acattccact
    16681 attagaggcg tttatggctt tacgcgccgc cagagcagcg gattagtgct aagggttttg
    16741 tcatcccgct ggcattgcag cagtattcct tcggctttaa ttaccgcccc ttcagaataa
    16801 ttttgatcct gataaacgca gcactgagta cagggctgcg ctgactgccc gcctgaactg
    16861 aacacttctg gcggtacgtt tacctccacg tccggacgat aatgcgggtt agccagtgca
    16921 attaatggaa atgctaatac tacggcgaac aatgctcgac tcacagggaa ctccttaacg
    16981 ttattgtctc tgcaactgat aacggtagcc gggtggcaaa actttagcgt ctgagttatc
    17041 gcatttggtt atgagattac tctcgttatt aatttgcttt cccgggtcat ttttttcttg
    17101 cttaccgtca cattcttgat ggtatagtcg aaaactgcaa aagcacatga cataaacaac
    17161 ataagcacaa tcgtattaat atataagggt tttatatcta tggatcagac atattctctg
    17221 gagtcattcc tcaaccatgt ccaaaagcgc gacccgaatc aaaccgagtt cgcgcaagcc
    17281 gttcgtgaag taatgaccac actctggcct tttcttgaac aaaatccaaa atatcgccag
    17341 atgtcattac tggagcgtct ggttgaaccg gagcgcgtga tccagtttcg cgtggtatgg
    17401 gttgatgatc gcaaccagat acaggtcaac cgtgcatggc gtgtgcagtt cagctctgcc
    17461 atcggcccgt acaaaggcgg tatgcgcttc catccgtcag ttaacctttc cattctcaaa
    17521 ttcctcggct ttgaacaaac cttcaaaaat gccctgacta ccctgccgat gggcggtggt
    17581 aaaggcggca gcgatttcga tccgaaagga aaaagcgaag gtgaagtgat gcgtttttgc
    17641 caggcgctga tgaccgagct ttatcgtcat ctgggcgcgg ataccgacgt tccggcaggt
    17701 gatatcggcg ttggtggtcg tgaagtcggc tttatggcgg ggatgatgaa aaagctctcc
    17761 aacaataccg cctgcgtctt caccggtaag ggcctttcat ttggcggtag tcttattcgc
    17821 ccggaagcta ccggctacgg tctggtttat ttcacagaag caatgttaaa acgccacggt
    17881 atgggttttg aagggatgcg cgtttccgtt tctggctccg gcaacgtcgc ccagtacgct
    17941 atcgaaaaag cgatggaatt tggtgctcgt gtgatcactg cgtcagactc cagcggcact
    18001 gtagttgatg aaagcggatt cacgaaagag aaactggcac gtcttatcga aatcaaagcc
    18061 agccgcgatg gtcgagtggc agattacgcc aaagaatttg gtctggtcta tctcgaaggc
    18121 caacagccgt ggtctgtacc ggttgatatc gccctgcctt gcgccaccca gaacgaactg
    18181 gatgttgacg ccgcgcatca gcttatcgcc aatggcgtta aagccgtcgc tgaaggggca
    18241 aatatgccga ccaccatcga agcgactgaa ctgttccagc aggcaggcgt actgtttgca
    18301 ccgggtaaag cggctaatgc tggcggtgta gcaacgtcgg gcctggaaat ggcacaaaac
    18361 gctgcgcgcc tgggctggaa agccgagaaa gttgacgcac gtttgcatca catcatgctg
    18421 gatatccacc atgcctgtgt tgagcatggt ggtgaaggtg agcaaaccaa ctacgtgcag
    18481 ggcgcgaaca ttgccggttt tgtgaaggtt gccgatgcga tgctggcgca gggtgtgatt
    18541 taagttgtaa atgcctgatg gcgctacgct tatcaggcct acaaatgggc acaattcatt
    18601 gcagttacgc tctaatgtag gccgggcaag cgcagcgccc ccggcaaaat ttcaggcgtt
    18661 tatgagtatt taacggatga tgctccccag ggaacatttc ttatgggcca acggcatttc
    18721 ttactgtagt gctcccaaaa ttgcttgtcg taacgataac acgcttcaag ttcagcatcc
    18781 gttaactttc tgcgatagca gcagatatgc cagtaaagaa accccattag actatttttt
    18841 tgataatctt cttcgctttc gaacaactcg tgcgcctttc gagaagcaag cattatataa
    18901 tgccaggcca gttcttcttc aattgtcccg ttttgaaaag ctgtgcttga tatcgaggtc
    18961 atccatgata attccgccgc ccatattagc ttcgccgagg atttaccgga gcgattatta
    19021 gcgcaatcag agatatagtc tgagggaaaa acagcaaatt tattcaacaa ggcgataacc
    19081 tgctctgggg cttcctccat gtttgcttta aaggtattgg ctccatggtc gccagaaaga
    19141 aaatgctcca ttaaggcaca ataactttcg ctatcttcga taccccattg attctctaaa
    19201 gactcgcgtc ttttactgat gatatcgatc gagtcaaaag gaagcacatg atattggaag
    19261 gtatctttac caggttcagg ctttcgcggc cagaactcta gcgtttcaga ccattgctta
    19321 tgatagaatc gataaggtgc gatcaattgt agcgcctgta acttctcgat actgagcggc
    19381 tcaatacctt tagcctgata ataatgcagt tgttcttttt ttgctttaaa accggcccga
    19441 acaataagcc ccatcataat taatagataa agaaaagagc atcccgcggt aatcaggcct
    19501 ctttcattca aaccgttgga tgttatcgct gcgaacacaa acattacagc gacaacacat
    19561 gttaaataaa acccccactt acaaagcagc atggccttat tttctttaat catccgttca
    19621 aaattactat taaatatttc ccagccatta aaagaatact tctcgctccc aggatggttt
    19681 tgtaataaaa cttttttcat cacattccct gttattacat actttagagg cgataatttt
    19741 atccatgcaa aaaaaatatc caacgaattt tcttgatccg ctggatattt cagaatatg
//
LOCUS       DAKISA010000086        19368 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.086, whole genome shotgun sequence.
ACCESSION   DAKISA010000086 DAKISA010000000
VERSION     DAKISA010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19368
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.086"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(32..1861)
                     /gene="glmS"
                     /locus_tag="OVW45_003068"
     CDS             complement(32..1861)
                     /gene="glmS"
                     /locus_tag="OVW45_003068"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCV5527457.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLDGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2023..3393)
                     /gene="glmU"
                     /locus_tag="OVW45_003069"
     CDS             complement(2023..3393)
                     /gene="glmU"
                     /locus_tag="OVW45_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCV5527458.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKVGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3744..4163)
                     /gene="atpC"
                     /locus_tag="OVW45_003070"
     CDS             complement(3744..4163)
                     /gene="atpC"
                     /locus_tag="OVW45_003070"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCV5527459.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4184..5566)
                     /gene="atpD"
                     /locus_tag="OVW45_003071"
     CDS             complement(4184..5566)
                     /gene="atpD"
                     /locus_tag="OVW45_003071"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCV5527460.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5593..6456)
                     /gene="atpG"
                     /locus_tag="OVW45_003072"
     CDS             complement(5593..6456)
                     /gene="atpG"
                     /locus_tag="OVW45_003072"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCV5527461.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6507..8048)
                     /gene="atpA"
                     /locus_tag="OVW45_003073"
     CDS             complement(6507..8048)
                     /gene="atpA"
                     /locus_tag="OVW45_003073"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCV5527462.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8061..8594)
                     /gene="atpH"
                     /locus_tag="OVW45_003074"
     CDS             complement(8061..8594)
                     /gene="atpH"
                     /locus_tag="OVW45_003074"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCV5527463.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8609..9079)
                     /gene="atpF"
                     /locus_tag="OVW45_003075"
     CDS             complement(8609..9079)
                     /gene="atpF"
                     /locus_tag="OVW45_003075"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCV5527464.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9141..9380)
                     /gene="atpE"
                     /locus_tag="OVW45_003076"
     CDS             complement(9141..9380)
                     /gene="atpE"
                     /locus_tag="OVW45_003076"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCV5527465.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9427..10242)
                     /gene="atpB"
                     /locus_tag="OVW45_003077"
     CDS             complement(9427..10242)
                     /gene="atpB"
                     /locus_tag="OVW45_003077"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCV5527466.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10251..10631)
                     /gene="atpI"
                     /locus_tag="OVW45_003078"
     CDS             complement(10251..10631)
                     /gene="atpI"
                     /locus_tag="OVW45_003078"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCV5527467.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAYTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11248..11871)
                     /gene="rsmG"
                     /locus_tag="OVW45_003079"
     CDS             complement(11248..11871)
                     /gene="rsmG"
                     /locus_tag="OVW45_003079"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCV5527468.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11935..13824)
                     /gene="mnmG"
                     /locus_tag="OVW45_003080"
     CDS             complement(11935..13824)
                     /gene="mnmG"
                     /locus_tag="OVW45_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCV5527469.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLVGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14203..14646)
                     /gene="mioC"
                     /locus_tag="OVW45_003081"
     CDS             complement(14203..14646)
                     /gene="mioC"
                     /locus_tag="OVW45_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCV5527470.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14736..15194)
                     /gene="asnC"
                     /locus_tag="OVW45_003082"
     CDS             complement(14736..15194)
                     /gene="asnC"
                     /locus_tag="OVW45_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCV5527471.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLAEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15346..16338
                     /gene="asnA"
                     /locus_tag="OVW45_003083"
     CDS             15346..16338
                     /gene="asnA"
                     /locus_tag="OVW45_003083"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCV5527472.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            complement(16343..17794)
                     /gene="viaA"
                     /locus_tag="OVW45_003084"
     CDS             complement(16343..17794)
                     /gene="viaA"
                     /locus_tag="OVW45_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCV5527473.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(17788..19284)
                     /gene="ravA"
                     /locus_tag="OVW45_003085"
     CDS             complement(17788..19284)
                     /gene="ravA"
                     /locus_tag="OVW45_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCV5527474.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIHQAQQC"
BASE COUNT         4627 a         5126 c         4848 g         4767 t
ORIGIN      
        1 gtcggcagcc cttttttatc tatgatagtg attactcaac cgtaaccgat tttgccaggt
       61 tacgcggctg gtcaacgtcg gtgcctttga tcagcgcgac atggtaagcc agcagctgca
      121 gcggaacggt gtagaagatc ggtgcaatca cctcttccac atgcggcatc tcgatgatgt
      181 gcatgttatc gctacttaca aaacccgcat cctgatcggc gaagacatac aattggccgc
      241 cacgcgcgcg aacttcttca atgttggatt tcagtttttc cagcaattcg ttgttcggtg
      301 caacgacgat aaccggcata tcggcatcaa taagcgccag cggaccgtgt ttcagttcac
      361 cagcagcgta ggcttcagcg tgaatgtagg agatctcttt cagcttcaat gcgccttcca
      421 gtgcgattgg gtactgatcg ccacggccca ggaacagtgc gtgatgtttg tcagagaaat
      481 cttctgccag agcttcaatg cgtttgtcct gagatagcat ctgctcaata cggctcggca
      541 gcgcctgcag accatgcaca atgtcatgtt caatggaggc atccagacct ttcaggcgag
      601 acagcttcgc caccagcatc aacagcacag ttaactgagt ggtgaatgct ttagtggacg
      661 ccacgccgat ttccgtaccc gcgttggtca tcagcgccag atcggattcg cgcaccagag
      721 aagaacccgg aacgttacag attgccagcg aaccaaggta acccagctct ttcgacagac
      781 gcaggcccgc cagggtatcc gcggtttcgc cagactgtga caaggtgatc atcaggctgt
      841 tacgacgcac ggcagattta cgatagcgga attcagaggc gatttcgacg tcgcacggaa
      901 tacctgccag cgattcaaac cagtagcggg aaaccatacc ggagttataa gaagtaccac
      961 aggcgaggat ctgaatatgc tcaaccttcg acagcagttc gtcggcgttc ggtcccagct
     1021 cgcttaaatc aacctgaccg tggctgatgc gcccggtaag ggtgtttttt atcgcgttcg
     1081 gctgttcgta gatctctttc tgcatgtagt gacggtaaat acctttatcg cccgcgtcat
     1141 attgcagatt ggattcgata tcctgacgtt ttacttccgc gccagtttta tcgaagatgt
     1201 ttaccgaacg gcgagtgatt tccgcaatat cgccctcttc aaggaagata aagcgacggg
     1261 tcaccggcaa cagcgccagc tggtcagaag cgataaagtt ttcgcccata cccaggccaa
     1321 tcaccagcgg actaccagaa cgtgccgcca gcagggtatc cgggtgacgg gagtccatga
     1381 tcactgtacc gtacgcaccg cgcagctgcg ggatagcacg cagaacagcc tcacgcagag
     1441 tcccgccttg tttcagctcc cagttcacca gatgggcaat cacttcggtg tcggtttcag
     1501 aaacgaaggt atagccacgc gcttttaact cttcacgcag cggttcatgg ttttcgatga
     1561 tgccgttatg caccaccaca atgtgttcag aaacatgcgg atgcgcgtta gcttctgaag
     1621 gttcaccgtg ggtcgcccag cgagtgtgag caataccggt gccgccatcc agaggatgtt
     1681 cttccgctgc ctgagccagc atctggactt taccgaggcg acgcaggcgg gtcatatgac
     1741 cttccgcatc aacaacggcc agaccggcag agtcatatcc gcggtattcc agacgacgta
     1801 aaccttcaag aaggatttct gctacatcac gttgcgcgat cgcgccaaca attccacaca
     1861 tagtttttga ttccgattta tatcgttgtc ggtcaacctg tatgcccgtt ttttcgggcg
     1921 ccccgagcct tgtagagagt ggggttattt ttatagttac tgcttgtggg agggagatta
     1981 tgttatctcc tcatcccaga tgaccggatt acccggccag aattactttt tctttaccgg
     2041 acgacgccag ccttctttct gagtctgcgg cacacggctg atagctaatg cattttcgcc
     2101 gacattacgc gtcacagttg tacccgcagc aatggtcgcg cctttgccta ctgttaccgg
     2161 ggccaccagc tgagtgtcgg aaccgacaaa cacatcgtcg ccgataatgg tcttaaattt
     2221 attcgcacca tcgtagttgc aggtaatggt tcccgcgccg atgttaacgt tatcgccaat
     2281 ttccgcatcg cccaggtaag tcagatgccc gactttcgag cctttaccca gacgcgcttt
     2341 tttcatctca acgaagttac caacgtgtgc accttccagc aactcagcac caggacgcag
     2401 acgagcaaac gggccaatag tacaggccgc tgccagattc gcgtcttcca caacggtata
     2461 cggactgatt tcgcaatcat cgccaatcac gctgttttta atcacgcaac cggtgccgat
     2521 tttcacgcga tgaccgagag tcacgttgcc ctcgatgata acgttagtat caatttcaac
     2581 atcgcgcccg tgagtaagcg taccgcgcag atcaaaacgc gccggatcgc gcagcataac
     2641 gcctgctaac agcagttttt cagcctgttc ggactggtaa acgcgctcca gacgggagag
     2701 ttgcaggcgg ttattcacac cttctacttc gcttaaacgt tgcggatgaa cggcgacgat
     2761 ttcacgccct tcctgatacg ccagcgcaat aatgtcggtg atgtagtatt cgccctgagc
     2821 attattgttg gtcagcttcg ccagccagcg tttcatatct gcgccgttag cgatcagaat
     2881 gccggtgttg atctcctgaa tctgacgctg ctcgtcggtg gcatctttgt gctcaacaat
     2941 gccggtaact ttgccatttt cacgggtgat acgtccataa ccggtcggat catccagttt
     3001 caccgtcagc agaccaatgc caccctgcgg tttagcatca cgcaggcgct ggagtgtttc
     3061 gacagagatc agcggcacgt cgccgtagag cattaaaatg tcttcatcat cggcaaagaa
     3121 aggtgcggcc tgctgcattg catgacccgt acccagctgc tctgcctgaa gcacccagtt
     3181 aaggttgtcg tctttcagcg cctgtttaag cagatcgccg ccgtgaccgt acaccaagtg
     3241 aacgtgcgct gcgcctaatt cattcgcagc atcaatgaca tgctgaacca tcgctttccc
     3301 ggcaagggta tgcagcactt tcggaagatc ggaatacatg cgcgtgcctt tgcctgcggc
     3361 aaggatcact acgctcatag cattattcaa catacgcgtc ctgactgtaa tttgagaacg
     3421 aatttaaacc gcttcacctt gaaaaaacta catttttttc atcgtgaaat ggacagagga
     3481 taaattgttc aataacggat tatccccgct gacgaaagtg ccattttcga ccatcgtcac
     3541 gccttttgtc tgcagaaaat taaggcagta ccttcattta agcgtcaggt ggatgttttt
     3601 gctcttattt cgatcaatga ataaacggga aaacaggggt gttttatatc agcaggatct
     3661 atgtgaacgc tcttcaggac ggatcacgcg taaaaaaaag ccagcctgtt tccagactgg
     3721 cttttgtgct tttcaagccg gtgttacatc gcttttttgg tcaactcgat aacgcgcagc
     3781 tgcgcgatcg ctttggccag ttccgcagac gcctgagcgt aatctacgtc gccgtgagag
     3841 ctgctaatgt gctcttcagc cttacgtttc gcttccatgg ctcgcgcttc gtcgagatcc
     3901 tggccgcgaa ttgcggtgtc ggccagaacg gtcacgttgc caggctgcac ttcaagaatg
     3961 ccgccagaca gatagataaa ctcttcgtga ccgtgctgtt tcacgatgcg aatcatacca
     4021 ggcttaatgg cggtgagcag cggtgcgtgg ccagggtaga tccccagttc accttcgcta
     4081 cccgttacct ggattttctc gaccagacca gagaacattt gttgctctgc gctgacgacg
     4141 tccaggtggt aagtcattgc catatcaccc tccgattaag gcgttaaagt tttttggctt
     4201 tttccacagc ttcttcgatg gaaccgacca tgtagaacgc ctgctccggc aggtgatcgt
     4261 attcgccttc catgatgcct ttaaagccac ggatggtgtc tttcagggag acgtatttac
     4321 ccggagaacc ggtgaatact tctgccacga agaacggctg ggacaggaag cgctggatct
     4381 tacgagcacg cgctaccacc agtttgtctt cttcagacag ttcatccata cccaagatgg
     4441 cgatgatgtc tttcagttcc tgataacgtt gcaggatgga ctgaacgcca cgcgcggtgt
     4501 cgtagtgttc ctgaccaacc accagcgggt ccagctgacg gctggtggag tccagcgggt
     4561 caacggccgg gtagataccc agagacgcga tctgacggct cagtaccacg gttgcgtcaa
     4621 ggtgcgcaaa ggtggttgcc ggagacgggt cagtcaagtc atccgcaggt acgtataccg
     4681 cctgtacgga ggtgatagaa ccagttttgg tggaggtgat acgttcctgc agaacgccca
     4741 tctcttccgc cagggtcggc tgataaccta ccgctgaagg catacggccc agcagtgcgg
     4801 atacttccgt accggccagg gtgtaacgat agatgttgtc aacgaacagc agaacgtcac
     4861 gaccttcgtc acggaatttc tcagccatgg tcagaccggt cagagcaacg cgcagacggt
     4921 ttcccggcgg ctcgttcatc tggccataca ccagggatac tttgtcgata acgttggagt
     4981 cggtcatttc gtggtagaag tcgttaccct cacgagtacg ttcacctacg cccgcaaaca
     5041 cagagtaacc ggagtgctcg atcgcgatgt tacgaatgag ctccatcatg tttacggttt
     5101 tacctacacc cgcaccaccg aacagaccaa ctttaccgcc cttagcgaac ggacacatca
     5161 ggtcgataac tttgataccg gtttccagca gttcctgaga gtttgacagc tcttcgtagg
     5221 aaggtgctgc gcggtgaatc gcccaacgct cttcttcacc gatctcgcct ttcatgtcga
     5281 ccggttcacc cagtacgttc atgatacggc ccagagtcgc tttacctacc gggacttcga
     5341 tcgggtgttc gaggtctttt acatccagac cgcgacgcag accgtcggag gaacccattg
     5401 cgatggtacg cacgataccg ccgccgagct gctgctgaac ttccagcacc agacgctcat
     5461 taccattttg cacctcaaga gcatcgtaca cgcgcggtac ggcatcctga gggaattcga
     5521 cgtcaactac ggcgccgatt acctggacaa tctttccagt agccatctta aatcctctac
     5581 gaaataacct gtttaaaccg cggcggcccc cgagacgatc tcggtgagtt cctgagtaat
     5641 gctggcctga cgagctttgt tgtataccaa ctgcagctct ttaatcaggc tgccgccatt
     5701 gtcggtcgcg gctttcatcg ccaccatacg ggcggcctgc tcgctggcca ggttttcaac
     5761 cacgccctga taaacctgag attcgacata acgacgcagc agggtatcca gcaacgcctt
     5821 cggatcgggt tcgtacaggt aatcccagga tttatgtttc agatcatcat catctgatgc
     5881 cggtaacggc agcagctggc tgatggtcgg aacctgagac atggtgttaa taaatttgtt
     5941 gctgacaatg taaagcttgt ccagacggcc ttcgtcgtaa gcctgcaaca tcacttttac
     6001 cggaccgatc agttcggaca gggaagggtt atcccccatg ccggtgacct gggcaacaac
     6061 attgccgccc acggagttga agaacgacac gcctttcgag ccgatcattg cgaggtcgca
     6121 ttgaacgcct ttgtcggtcc aggtcttcat ttccgccagc agttttttga acaggttaat
     6181 gttcaaacca ccgcacaaac cacggtcggt cgacaccacc aggtagccca cgcgtttaac
     6241 gtcgcggtct tccaggtaag ggtgcttata ttccagatta ccgtgtgcaa ggtgaccaat
     6301 cactttgcgc atggtttctg cataaggacg gctggccgcc atgcgatcct gcgatttacg
     6361 cattttggaa gcggcgacca tctccatcgc tttagtgatc ttttgcgtgt tctggacgct
     6421 tgcgatctta ctacgtatct cttttgcgcc ggccatgagc ttctcctcaa tgccttgcgg
     6481 cctgccctaa ggcaagccgc cagacgttac caggattggg ttgctttgaa ggaatcgagg
     6541 atgcctttca gcttgccttc gatttcgtcg ttgtagccac cggtctggtt gatctcttgc
     6601 atcaacggag cgtgatcacg gtcgacgtaa gccagcagag cggcttcgaa gctgccaatt
     6661 ttcgacagtt caacatccgc caggtaacca cgttctgctg cgaacagaac cagagactgc
     6721 tgcgcaacgg acatcggcgc atactgtttc tgtttcagca gttcggtcac tttctgaccg
     6781 tggtcaagct gcttacgtgt tgcatcgtca aggtcggatg caaactgaga gaacgctgcc
     6841 agttcacgat actgtgccag agcggtacgg ataccaccgg acagtttttt catgatcttg
     6901 gtctgtgctg caccaccaac acgggatacg gaaatacccg ggttaaccgc aggacgaata
     6961 ccggcgttga acaggttggt ttccaggaag atctgaccat cggtaatgga gattacgttg
     7021 gtcggaacga acgcagaaac gtcacccgcc tgagtttcga taatcggcag tgcggtcaga
     7081 gaaccggttt tccctttcac ttcacctttg gtgaaggctt caacgtattc ggcgttaaca
     7141 cgtgcagcac gctccagcag acgagagtgg aggtagaaaa cgtcgcccgg gaatgcttca
     7201 cgtcctggcg gacgacggag cagcagggag atctgacggt aagcaacagc ctgtttagac
     7261 aggtcatcgt aaatgatcag cgcatcttca ccgcggtcac ggaagtattc gcccattgcg
     7321 caaccggcat acggtgccag gtattgcagt gcagcggatt cagacgcggt tgctaccaca
     7381 acgatggtgt tagccagtgc gccgtgctct tccagtttac gtaccacgtt agaaatggtg
     7441 gacgctttct ggccgatagc gacatagata catttgatac cggaatcgcg ctggttgatg
     7501 atggcatcga tagccagtgc ggttttacct gtctgacggt caccgatgat caattcacgc
     7561 tgaccacgac cgattgggat catggagtca acggctttat aaccggtctg taccggctga
     7621 tctacggact gacgttcgat aacgcccgga gcgattgctt ctacagcaga gaagccgtcg
     7681 tgatccagcg gacctttacc gtcgattggt gcacccagag tgttaaccac acggcccagc
     7741 aggccacggc caaccggaac ttccaggata cggccagtac acttaacttt catgccttcg
     7801 gcaaggtcag cgtacggacc cataacaacc gcacctacag agtcgcgctc gaggttcagt
     7861 gcgatagcgt aacggttacc cggcagggag atcatttcac cctgcataca atcggccagg
     7921 ccgtgaatgc ggataacacc gtcacttaca gaaacaatag taccttcgtt gtgagcttca
     7981 ctcacaacat tgaactgagc aatgcgctgc ttgatcagtt cgctgatttc ggtggaattc
     8041 agttgcatgc tccagtcccc ttaagactgc aagacgtctg caaggcgctc aagacgaccg
     8101 cgtacgctgc catcaatgac catatcaccc gctcggatga taacgcctgc cattacagac
     8161 ttatcgattt tgcaattcag cttaactttg cgtgacagac gtttttccat cgcagcagaa
     8221 attttcgcga gctgttgttc actcagtgcg gcagcggaaa tgacgtctac ctcagcggta
     8281 gcctcactca cggcacgaag gtgaataaac tgctccagaa catccgggag cgcgttaaga
     8341 cgaccatttt cagccattac ccgaatcagg ttctgaccgt tttcgtccag ttgctcacca
     8401 caaactgcga taaacgactc ggcgagcgtt tctggcgcaa gcgcgccaga gagaagctct
     8461 gccatttgtt cgtttttggt tacctcggcg gcaaacgcca gcatgtcctg ccagcgttct
     8521 acactttggt gttcgacggc aaagtcaaaa gctgctttgg cgtaggggcg agctaccgta
     8581 ataaattcag acatcagccc ctccctcctt acagttcagc gacaagttta tccacgatgt
     8641 cgctgttagc agcttcatcc acggaacgtt cgatgatctt ctcggcgcca gcaacagcca
     8701 ggatagcaac ttgcttacgc agctcttcac gggcacgttt acgctcggct tcaatttctg
     8761 cctgcgcctg ggccacgatt ttagtacgtt cctgttctgc ctcagctttc gcttcgtcca
     8821 gaatctgcga gcggcgtttg ttcgcctgct cgatgattac ctgggcttcc gctttcgctt
     8881 ttttcagctg gtcggtcgcg ctggcctttg caaggtcaag gtccttatgt gctcgttctg
     8941 cggaagcaag gccgtcagca atttcttttt gacgtttttc gatggctgcc attaatggcg
     9001 gccatacgta cttcatgcag aacagaacga acaggacaaa cgcgatggcc tggccgagga
     9061 ttgttgcgtt aagattcaca gcacaatgcc tctatttagt taacgttctg atattgctct
     9121 ttaaataaaa gcaacgctta ctacgcgaca gcgaacatca cgtacagacc cagacctaca
     9181 gcgatcatcg ggatagcatc caccagaccc ataacgataa agaactgagt acgcagcaga
     9241 ggaatcagat caggttgacg cgctgcgcct tccaggaatt tccccccgag gatgccgata
     9301 ccgatcgcag caccgattgc cgccagaccc atcatcacag cggcagccat gtacagcaga
     9361 tccatattca ggttttccat gacagtctcc agtttgtttc agttaaaacg tagtagtgtt
     9421 ggtaaattaa tgttcttcag acgccatcga cagatagacg atcgtcagaa ccatgaagat
     9481 gaaggcttgc agcgtaatga tcaggatgtg gaaaatggcc cacggcacat tcaggatcca
     9541 ctgtgaccac cacggcaaca gaccagcaat cagaatgaaa atcagctcac cggcatacat
     9601 gttaccgaac agtcgcaaac cgagtgaaac tggtttggac agcaggctta ccccttcaag
     9661 gattaagttg acaggaatga acgcccagtg attgaacggc tgcagcgtca actctttcgt
     9721 gaagccgccg atgcctttca ttttgatgct gtagaacaga atcaggataa atacgcccag
     9781 tgccatagac agcgttacgt tcacgtccgc agacggaacc acacgcagtg caggcagacc
     9841 cagtacatgt tcagcaatgt acggcagcag gtcgataggc agtaaatcca tcaggttcat
     9901 caggaatacc cagacgaaga tcgtcagggc cagcggagca atcagcttgc ttttgccatg
     9961 gtacatgtct ttcacgctac cattaacaaa gccgatcacc agctcaatcg cggtctgaaa
    10021 cttacctggc acaccgctgg tcgccttttt ggctacgcta cggaataaaa ccaggaacaa
    10081 cagacccagc accaccgaga agaacatgga gtcaatattg attgtccaga aggtggctgg
    10141 ggggttttgt ggatccacca gcgagaatgt acgcaggtcc agctgaaggt tattcaggtg
    10201 gtgtcctatg taatcctgcg gcgtcatatt ttctgaagcc atgatgcctt ttaccctttg
    10261 ttgttaatta cagccggtgc cagtatctga accaccagca ccaaaaccca cgtaacgatc
    10321 agcggcaaga ataccgcctt taaaaccgcc aacgccacca ccagtaacac caacatcgcc
    10381 agaactttga aagcttcgcc aaatgcgaat gtccaggcca cccggccttt cgctggtgta
    10441 tacgcctggt gacgccaggc aaatatcata aacaaaacgt taggcagaaa gactgccagg
    10501 cccccgctta ttgcagagac gccccagaag gggtctttga ggctgaacag caatccactt
    10561 gctatcacca ccagtaactg aacgagcaga agcttccgag caacgtttcg actcacgagc
    10621 gacacagaca tcacgttttt cactcctgct cccttcgagg tatgccgcgt gtcgtataaa
    10681 actttcttta aggcttagag tcaagcatca aaaagcggtc aaattatacg gtgcgccccc
    10741 gtgatttcaa acaataagta gccaaaaggt gaataaatgt ttaaatattt ttccagtgca
    10801 tacaattgcg acttttccgc taaccctgtt cgatcatgaa aaactgtaaa taacgcgtaa
    10861 acactagtga taaagcgtgc ttcagatcac atattgcgca tgttcgcgca cagtatattt
    10921 atttacttgg caaatgatgc ctttgcaagt ttatgatatt tcagtctaaa aacagatact
    10981 gttttaataa atgacattta cacaacaaaa accacccatt gacattttta ataatgtttt
    11041 aacagccaat gatggttctt agcgccgatt tttagcagac tgatattttc actaatgact
    11101 tattttctgc ttaccaaaaa aagccacgtt atcttgttga tacaaaagag tgaacgtggc
    11161 gttaaatgta accagttata tcagtagaaa acttggttgt tgttaacagt ctaaccggtc
    11221 aattttttat gatttttttg ataaaaatta aattttattt gctttaatca ccaccagatg
    11281 acgttcgcca tccagagctg gaacctgaag tttaaccact gattcgacct gatattcttc
    11341 gggcaacaaa gcgatttcat cttccggcat ttgccctttc agcgcgtaga aacggccttg
    11401 ctcaccagga agatggtggc accagctcac catatcgttc agagaggcaa aagcgcggct
    11461 aattacgcca tcaaatggcg gctctgaagg aaactcttct accctgctct gtactggctc
    11521 aatattctcc agtttaagct catgttgcac ctgacgaagg aaacgcacgc gtttaccaag
    11581 gctatccaac agagtgaaat gggcttcagg acgcacgata gagagtggaa tgcctggcag
    11641 tcctggtccg gtgccgacat cgataaaccg ttcaccttgc agatacggtg ccaccacaat
    11701 gctatcgaga atatggcgta ccagcatctc attaggatcg cggaccgaag tcaggttgta
    11761 cgctttgttc catttatgca gcatattcac gtaggcaata agctggtttt tctggtgatc
    11821 ggtaagcgaa atacctgcgt ctttcagcag taaggagagt ttgttgagca cggtgattac
    11881 ctgttcttga tgcgttgcct ggtaagcggg tgcttaccag gcatttttaa tgcgttatgc
    11941 gctacgacgc agcataccct gttttttcag ccacaccagc agaatggaga tagccgcagg
    12001 cgtgacgcca gaaatacgcg aagcctggcc gatagaggcc ggtttgtgat cgttaagttt
    12061 ggcgatcact tcgttagaaa gaccggatac ctggcggtaa tccagtgtcg cgggtagcag
    12121 ggtgttctcg ttacgcagct gcttttcgat ctcatcttgc tggcgcgcga tataaccttc
    12181 gtatttaacc tgaatctcaa cctgttccgc cgcctgttcg tctgtcaacg caggggcaaa
    12241 cggcgtcagc gtggttaatt tttcataagt catttccgga cgacgcagca gatcttcacc
    12301 actggcttca cgggaaagcg gcgcagtcag gtgagcattc acttcggctg cagcttccgc
    12361 cgacggggtt acccaggtcg atttcagacg ctgacgctca cgctcgatat tctcaagttt
    12421 ctcgttaaag cgcgcccaac gttcgtcatc caccaggccc agttcacgac cgatttcagt
    12481 caaacgcaga tccgcattat cttcgcgcag catcagacga tattctgcgc gcgaagtaaa
    12541 catacgatac ggttctttgg ttcctaaagt gcacaggtca tcaaccagta cgccgagata
    12601 cgcctgagaa cgcgccggag cccaaccttc tttgtcagca gacagacggg cagcgttaag
    12661 accgaccagc aaaccttgcg cagcggcttc ttcgtaaccg gtagtgccgt taatctgacc
    12721 agcaaagaac agcccctgga taaacttgct ctccagcgtc ggtttcaggt cgcgtggatc
    12781 gaagaagtca tactcaatgg cataacccgg acgcacgatc ttcgcgtttt ccatcccctg
    12841 catggagcgg acgatttgca tctgcacatc gaacggcagg ctggtggaga taccgttcgg
    12901 ataaatttcg ttagaggtca gcccttccgg ttcaaggaag atctgatgct gatttctgtc
    12961 ggcgaagcgc atgactttgt cttcgatcga tgggcagtag cgtgggccga caccttcgat
    13021 cacccctgcg tacattgggc tacgatcgag gttactgcgg atcacatcat gggttttctc
    13081 gttggtatga gtgatataac acggcacctg ctggggatgc tgggacgcat tgcccataaa
    13141 cgagaatacc ggcattgggt tatcgccatg ctgttgcgcc agcacactaa agtcgatagt
    13201 tcgagcatca atacgcggtg gtgtcccggt tttcagacga ccaacgcgca gcggcagttc
    13261 acgcaaacgg cgagaaagcg gaatggacgg cggatcacca gcacgaccac cgctgtaatt
    13321 atccagaccg atatgaattt taccgtcgag gaacgtccca acggtgagca cgacggcttt
    13381 ggcacggaac ttcagtccca tttgggtaac agcaccgacc acgcgatcgt tttcgacaat
    13441 aagatcttca accgcctgct ggaagatcat caggttcggt tggttctcca gcgccgtacg
    13501 taccgcctga cggtagagca cacgatccgc ctgagctcgg gtagcgcgaa ccgccggtcc
    13561 tttgcttgcg tttagtatcc taaactggat acccgcttga tcgatcgctt tcgccatcag
    13621 accgccgagt gcatccactt cttttaccag atgtcccttc ccaataccgc cgatcgccgg
    13681 gttgcagctc atctgcccca gagtgtcgat attgtgtgtc aaaagcagag tctgttgacc
    13741 catacgcgcc gcggccatcg cggcctcggt gcctgcatga cccccgccaa tgatgatgac
    13801 gtcaaaagga tccggataaa acatggtgat tgcctcgcat aacgcggtat gaaaatggat
    13861 tgaagcccgg gccgtggatt ctactcaact ttgtcggctt gagaaagacc tgggatcctg
    13921 ggtattaaaa agaagatcta tttatttaga gatctgttct attgtgatct cttattagga
    13981 tcgcactacc ctgtggataa caaggatccg gcttttaaga tcaacaacct ggaaaggatc
    14041 attaactgtg aatgatcggt gatcctggac cgtataagct gggatcagaa tgaggggtta
    14101 tacacaactc aaaaactgaa caaccgttgt tccttggata actaccggtt gatccaagct
    14161 tcctgacaga gttatccaca gtagatcgca cgatctgtat acttatttga gtaaattaat
    14221 ccacgatccc agccattctt ctgccggatc ttccggaatg tcgtgatcaa gaatgttgat
    14281 cttcagtgtt tcgcctgtct gttttgcacc ggaatttttg agttctgcct cgagtttatc
    14341 gatagcccca caaaaggtgt catattcacg actaccaata ccgattgcgc caaagcggac
    14401 cgcagaaaga tcgggcttct gttcctgcaa tgcttcatag aaaggagaaa ggttgtccgg
    14461 aatatctccg gcaccgtggg tggagctgat aatcagccag atccccgagg cagataaatc
    14521 ttccaacagc ggaccgtgca gcgtttcggt ggtaaaaccc gcctcttcca gcttttcagc
    14581 caggtgttct gctacatatt cggcaccgcc gagggtgctg ccgctgataa gagtgatatc
    14641 tgccataaac cgccaccttt attaagagtg gcgtattgta cgctgtgaac gcgttgggat
    14701 ctacctgtgg aaaagtatgg gattaaaaaa gccgattatg gcttgatggt acgcatgatc
    14761 gggttttgca ggacgatcaa tgtctcggtg gactgaattt catcaattgt ttggatcttg
    14821 ttgataagta catgctggag agcgtcgatc gaacggcaca ttacttttat aaagatgctg
    14881 tagtggcctg ttgtgtaata ggcttcagtg acttcagcaa ggctttccag ctttgccagc
    14941 gcggaagggt agtctttggc gctctttaat ataatgccga taaagcagcc tacgtcataa
    15001 ccgagctgct tcgggctgac atcaatacgc gccccggtaa tgatccccgc ctgcttcatt
    15061 ttctctactc gaacgtgaat cgtccccgga ctgacgccaa attgtttcgc cagttcggcg
    15121 taagcggtgc gcgcattgcc cattaatgct tccaggatgc cacggtccag attgtcgatc
    15181 agataatttt ccataggatt ttcttatgca gattgatgat tcattctatt ttagccttct
    15241 tttttaatga atcaaaagtg tgttaggctt tttattgaat gattattgca tgtttgtcgg
    15301 tttttgttgc ttaatcataa gcaacaggac gcaggagtat aaaaaatgaa aaccgcttac
    15361 attgccaaac aacgccaaat tagcttcgtg aaatctcact tttctcgcca actggaagaa
    15421 cgtctggggc tgatcgaagt ccaggcgccg attcttagcc gtgtggggga tggcacgcag
    15481 gataacttgt cgggctgtga aaaagcggtg caggtaaaag tgaaagctct gcctgatgcc
    15541 cagttcgaag tggttcattc actggcgaag tggaaacgtc agaccttagg gcaacacgac
    15601 ttcagcgcgg gcgaagggct gtacacgcac atgaaagccc ttcgccccga tgaagaccgt
    15661 ctttctccgt tgcactcggt ctatgttgac cagtgggact gggaacgcgt aatgggcgac
    15721 ggtgagcgtc aattctcgac tctgaaaagc acggtagagg cgatctgggc gggaattaaa
    15781 gcaaccgaag ctgcggttag cgaagagttt ggcctggcac cgttcctgcc ggatcagatc
    15841 cacttcgtac acagccagga gttactgtct cgttatccgg atctcgatgc caaagggcgt
    15901 gagcgggcga tagcgaaaga tcttggcgcg gtattccttg tcgggattgg cggcaagctg
    15961 agcgatggtc atcgccacga cgtgcgcgca ccggattatg atgactggag caccccgtca
    16021 gagctgggtc atgcgggtct gaacggcgat attctggtgt ggaaccctgt actggaagat
    16081 gcgtttgagc tttcttccat ggggatccgc gtggatgccg acacgttgaa gcatcagttg
    16141 gcgctgaccg gtgatgaaga tcgtctgcag ctggaatggc atcaggcgct gctgcgcggt
    16201 gaaatgccgc agaccatcgg cggcggtatc ggccagtctc gtttgaccat gctgctgctg
    16261 caactgccgc atatcggcca ggttcagtgt ggtgtatggc cagctgctgt tcgcgagaac
    16321 gtcccttctc tgctgtaata atttatcgcc gccagcgtct gagcaggcgg cttcgcatcc
    16381 cggtatcaaa gcgccagata tgatcgaaaa tgcgcatgat gccgggtttg ccgtgtgccg
    16441 acatcgccac ggcatgaaag cgatgctgat gtacccgctg cagctctttc actttactcg
    16501 tcacgtcgtc aggcaaccgc tgagcgataa aatcagaaat caccaccgca tcggcatcaa
    16561 accattccct gctttgcaag cgttccataa tggcgcgaaa acaactggca agatcggtgc
    16621 cgccacgaaa ctgctggctt aaaaaacgga ttgcttgttc gatgccttgg gggcctgaaa
    16681 gctcataacg gacgatctcg gtggaaaata gcataatata gcagcgccgg ttttcagcga
    16741 gagcaatgcg catcaaggcc aggcagaacg ctttcgcaca ctgttcatta aagccgccca
    16801 ttgagccgga agtatccaca cagacaataa acggcccgcg cggctgttca tcgtaatctt
    16861 tatgtaccac cggacgttcg atcacttttt cacgccacga ctcaccgtgc aggcgatagg
    16921 tgagcaactg tttttccacc agccgacggt aaaactcata ctccagttcc gttatcccta
    16981 gtgtcgccag ttctggcggc agaagacgta aaatatcatc gctttgttgc agaccatcaa
    17041 cctgctcagg aaccgtcgcc ggttcgcgca ccatggtgcg gaaggtttcc atctgcgcat
    17101 cgttgcgcgg tattgatttg gcttcccgag aacgccccag ctgctctgcc aggcgtttca
    17161 gttccggctg ttcgttaaga aattcaccgt atttcacaat caactgatag tcgccacgtt
    17221 taagctgacc ggcgctcata tcccacagac gaccagctgc agtattgtta tctgcgagaa
    17281 tcggttcaag ttgtccgctc agcgtcatgc gttcttgaac ttcactcaac agttgttcgc
    17341 gttcttcttc taatagctgt tgatttaacg tcgttgcttg cacgatcaga cttaaacgcc
    17401 aacgctggag aaaaagcgtg tgtaacgctg aagttattgt gctgttagca tcaaccaact
    17461 gccgggcttg ttctgcccat ggggaattca gacgatgcag taagtccagg atctgtggta
    17521 gctgcacaat aaactgcggc gtggagagga gctggctttg ctgatagcac atcacctctt
    17581 cggtgagttc cggcgggact cgggcatctt tcagccgact gcgcagcgcc tcacgccagc
    17641 ggggaacatc atcagtgatt gccgccttca ggcgtgggaa tttttcaaag aagaccgcca
    17701 gctgcggtga ggccagcagt gcgatgatca tctcttcgat caatccctct tcgctgacgg
    17761 ccagcatcac attaagcgta tccagcgtta gcattgttgt gcctggtgaa tctgtgcacc
    17821 gacatcctgt aggctggctt caatgcgacc taaccagtcg ccagggataa acagacattt
    17881 ttgctgttcg ctgaataaag cgtgttgctt gcgccagtcg ctttcaagct cttccagttg
    17941 ttgttttatt tcaccaggca gaccttcagc cgatgaaccg ggaagtgcca gcgtactgcc
    18001 ttgtaaactt acatcgcgaa cgacaagatg ttgggcgcta tcaacttcca gattcagttt
    18061 ctgggcaaag ccgataccgt tcagtttacc gcgaatttca ccacctttgc tcagccactg
    18121 ttccagcgcg ctacgctcaa aggagatatg aaccacttcc atatcatgca gttttaacgg
    18181 tttttgcagc agtagagtca gagtggaagc agtaacgtta acaggaagtt gatactgctg
    18241 acgacggctg aaaatgccgc ccagacgaat taccgttaag gctgttttat cgctttgttg
    18301 ctgctggagt tgcaggtgac gttgcacaat cgcgccaagg cgagtcaaca tcccttgctg
    18361 ttgccaggca tgaccggtca tcaatacatc aatttgttgt tgtatcaaat tcaggctttg
    18421 cgcgtcatac cacaggcaat ctttcagcaa aatgagatct accggggcaa cagcactgcg
    18481 accgctaaaa aaggcgctgg cctgcaataa tcggatcgct tttttccagc gacgatccga
    18541 gacataaggc gcatccggta atttatccag ttgctggcgc agcataaaaa tcagctcaaa
    18601 tacatgatcg ggcagcgtaa tttcaccaat ctctttctgc cagcgttcat attcttcatc
    18661 tgtgacctgc aaggcatcag gaaccggatt gtcgttttca tcctgttgac tggtcagcat
    18721 ggagcggaaa ttcgctttat cctgcacttt atctaaccac agacgaatca gcatgcggtc
    18781 atataacgct tccagactgc tgtctgcttc cggcagctcg ttggaggccg ccaccagcag
    18841 acgcatcggg attttttcta cgtgtgcacc gttgcggaac tggcgctcgt taatggcggt
    18901 gagcaaggta ttaagaattg ccgggcccgc tttccagatc tcatccagaa agacaatttc
    18961 tgcttccggc aggtaaccgc tggttaaacg ttcatagcgc ccttcatctt ttagcgcctg
    19021 aatagaaagg ggaccaaaaa cttcttccgg cgtggagaag cgggtcatca gatattcaaa
    19081 cgcgcgggca ttctgaaagg cgaattttaa gcgccgggcg atcaaacttt tggcaatacc
    19141 tggcgggcca aggaggaaca cactttcacc acttaatgcc gctaataaac acaagcggat
    19201 ggcgtggcta cgttcataaa gccccttttc caacgaactg ctcaggcggg aaattctttc
    19261 cgctaataaa tgagggtgag ccataatgaa gtggcgtcct ttcgtcaaaa gttctgcgta
    19321 aattgcgagt atagacgttt cttgctggtg gctaaaatag tctcaaag
//
LOCUS       DAKISA010000087        19097 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.087, whole genome shotgun sequence.
ACCESSION   DAKISA010000087 DAKISA010000000
VERSION     DAKISA010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19097
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.087"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(58..1707)
                     /locus_tag="OVW45_003086"
     CDS             complement(58..1707)
                     /locus_tag="OVW45_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCV5527475.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            2112..3509
                     /gene="ccp"
                     /locus_tag="OVW45_003087"
     CDS             2112..3509
                     /gene="ccp"
                     /locus_tag="OVW45_003087"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCV5527476.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCYTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLGVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            3720..5120
                     /locus_tag="OVW45_003088"
     CDS             3720..5120
                     /locus_tag="OVW45_003088"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCV5527477.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
     gene            5490..6314
                     /gene="gadX"
                     /locus_tag="OVW45_003089"
     CDS             5490..6314
                     /gene="gadX"
                     /locus_tag="OVW45_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCV5527478.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            6682..7410
                     /gene="gadW"
                     /locus_tag="OVW45_003090"
     CDS             6682..7410
                     /gene="gadW"
                     /locus_tag="OVW45_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCV5527479.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(7773..10886)
                     /locus_tag="OVW45_003091"
     CDS             complement(7773..10886)
                     /locus_tag="OVW45_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCV5527480.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(10911..12068)
                     /gene="mdtE"
                     /locus_tag="OVW45_003092"
     CDS             complement(10911..12068)
                     /gene="mdtE"
                     /locus_tag="OVW45_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HCV5527481.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            12128..12406
                     /locus_tag="OVW45_003093"
     CDS             12128..12406
                     /locus_tag="OVW45_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5527482.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYLGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(12407..12934)
                     /gene="gadE"
                     /locus_tag="OVW45_003094"
     CDS             complement(12407..12934)
                     /gene="gadE"
                     /locus_tag="OVW45_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCV5527483.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(13733..14305)
                     /gene="hdeD"
                     /locus_tag="OVW45_003095"
     CDS             complement(13733..14305)
                     /gene="hdeD"
                     /locus_tag="OVW45_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCV5527484.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            14560..14892
                     /gene="hdeA"
                     /locus_tag="OVW45_003096"
     CDS             14560..14892
                     /gene="hdeA"
                     /locus_tag="OVW45_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCV5527485.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            15008..15334
                     /gene="hdeB"
                     /locus_tag="OVW45_003097"
     CDS             15008..15334
                     /gene="hdeB"
                     /locus_tag="OVW45_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCV5527486.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            15398..16045
                     /locus_tag="OVW45_003098"
     CDS             15398..16045
                     /locus_tag="OVW45_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HCV5527487.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNMVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(16087..16617)
                     /locus_tag="OVW45_003099"
     CDS             complement(16087..16617)
                     /locus_tag="OVW45_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCV5527488.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(16773..17339)
                     /gene="slp"
                     /locus_tag="OVW45_003100"
     CDS             complement(16773..17339)
                     /gene="slp"
                     /locus_tag="OVW45_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCV5527489.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(17694..18809)
                     /locus_tag="OVW45_003101"
                     /pseudo
     CDS             complement(17694..18809)
                     /locus_tag="OVW45_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         5128 a         4219 c         4421 g         5329 t
ORIGIN      
        1 ctcctgaaga ggataatttt acagccagca ggaagttagc ggctggctgt aaaaatatta
       61 tggttcgccg tacaaaccaa ttaaacggcg taccacaccg ttagtccagc caaacccatc
      121 ctgcaacgga tactcgccac cgccgccttc gcggggaaca ccatcggcaa tatggtattt
      181 ctcgatcatt ttgtgctgtt ccagatagaa ctgattcacc gttttcagcc agctgcgcgc
      241 gatttcatca cccagaaggt catcgccata cattttaaat ccctgaattg ccatccattg
      301 taacggtgcc cagccgttgg gtttatccca ctgttcaccg gtttcgtact cgcttgccag
      361 aatcccgcca ggtgtcagta accggctgcg tacggcgttt gccagacgat cggcctgttc
      421 atggttcgcc atgccgacat agagcggcac aatggcggca gcggaaaaca gcgccagttg
      481 ttcgcgtcgc cagtcgtaat cgcggtagat gccgttttca tcatcccaga ggtaacggtt
      541 taccgcatcg cgacgggcac tggccttctg gcggaacagc gcttctgtct ctttctcgcc
      601 tttcagcgcc gagatgttgg cgatggcgct ctccagttta aacaggaagg cattcagatc
      661 gatggggatg aactgggtgg tacgaatgct cgccagacga ccagtatcac gcagccaacg
      721 ggaagagtaa tcccaaccgg aggccgcccc cgcgcgtaaa tcgcggtaca cctcgttggg
      781 cgggcgacca gaatgtttcg cggtttcaac gtcctcaagc caggattcgt cacgcggcgt
      841 gtcgcgatcg tcccagtaac ggttgagcag cgatccgtcc ggcatccgca caacatggcg
      901 ataggcctga ttagggatca acgattctgc accgtccatc cagaaggcat attccatttt
      961 aaggtggtcg agatagcggc gcgcaccgcg tacaccatct tcttcaaaca actccaccat
     1021 cagcgcaaaa accggtggtt gcgatcggct caaataatag gtgcggttgc cgttggggat
     1081 gtgaccatag ttttcgatca tccaggcgaa gttatcggcc atgcatttca gcaaatcttc
     1141 ccgaccactt tccgccagcc ccagcatggt gaaataggag tcccaatagt aggtttcgct
     1201 aaaacggccg cccgggacaa tatatgactg cggcagcgcc agtagagaag accacggaat
     1261 gtgatcctgt ggttcgcggg ttagcaccgg ccacagctgg tcgatatgct ctttcaggga
     1321 attttgcggg tccgatacat actcgctgga gtagacctcc ggcagccaga agtgattttc
     1381 aacaaacttg cgcaagtcaa aatcacgatg acggcgcact ttacggtagc ggattaaaat
     1441 atccagcggg tccattttag gtgcgcagtc gggaaaggtt ttactgtccg ggaaaatttt
     1501 cgccgactga acatgctcga acagttcgag atagcgatcg gcaggcgtca gcgcatcaga
     1561 ggcgggcagc ccttcaatca tctcgggttc cggttctgcc tcgatcatct catccagttt
     1621 taattcatac gggtccagct catagcagag atcgacttcg atcatcagtt cgtctggatt
     1681 agggttttga attttctgat tgagcataag gatccaacct ccgaaattcg tcgctaatta
     1741 tcgggctttt ctttaagggt agtcattcgc tccgcctggt gggcgacaaa gcgtgcggtg
     1801 gatcacgttt attaacctgt caaaaaatat aacgcactaa tatccatgga atttataaaa
     1861 atacggattt cgtgttgttt ttcattttca gaacattcaa tcctgctctc gccgcaatca
     1921 tgatatgcca ttcctgatct gggtaatagg cacaggctat cttattgata gtttatattc
     1981 atgtaattga ttgattccta tcaaaaagcg tatctcactg ttttttatga tggctttgtc
     2041 tcgcagagac gtttgcagca ttctggaaaa ttttctgggt cacaataact caccaccgtg
     2101 agggaagaat aatgaaaatg gtctcacgta ttaccgcgat cggcctggct ggcgtcgcga
     2161 tttgctattt agggttatct ggttatgtgt ggtaccacga taataaacgc agtaaacagg
     2221 ccgatgttca ggcatccgct gtcagtgaaa ataataaggt tttaggtttt ctccgcgaaa
     2281 aaggatgcga ctattgctac acgccttcgg cagaattacc cgcctattat tatattcctg
     2341 gcgcgaaaca gttgatggat tacgacatta agcttggata taaatctttt aacctcgagg
     2401 ccgtgcgtgc ggcactgctg gctgataaac ccgtttcgca aagcgatctg aataagattg
     2461 aatgggtgat gcagtatgaa actatgccgc caacgcgtta taccgcgctt cactgggcgg
     2521 gtaaggtgag tgatgaagag cgggcggaaa tactggcctg gattgcaaaa cagcgcgcgg
     2581 aatattacgc cagcaatgat actgctccgg agcatcgcaa tgaaccggtg cagcccatcc
     2641 cgcaaaaact gcctaccgat gcgcaaaaag tggcgttggg ctttgcgctg tatcacgatc
     2701 cccgtttatc ggctgatagc accatttcat gcgctcattg ccatgcgttg aatgcggggg
     2761 gcgtcgatgg cagaaaaaca tcgattggtg ttggtggcgc agttgggccg attaacgcgc
     2821 cgacggtatt taactcagta tttaacgttg agcagttctg ggatggtcgt gcggcaacat
     2881 tgcaggatca ggctggtgga ccgccgttga acccgattga aatggcgtcg aaatcttggg
     2941 acgaaattat tgctaagctg gaaaaagatc cgcagcttaa agcgcagttc ctcggcgtct
     3001 atccgcaagg tttcagtggc gaaaatatta ctgatgccat tgctgaattt gagaaaacat
     3061 taattacgcc ggattcccca tttgataaat ggttgcgtgg agatgaaaat gcgctgacgg
     3121 cgcaacagaa aaaaggctat caattattta aagataataa atgtgcaact tgtcatggtg
     3181 gtattattct cggtggacgt tcctttgaac cgttggggct gaaaaaagac tttaactttg
     3241 gggaaattac ggcggcggat attggtcgta tgaatgttac taaagaagag cgtgataaat
     3301 tgcgtcagaa agtacccggt ttacgtaacg ttgctttaac ggcaccgtac ttccatcgcg
     3361 gtgacgtgcc gacgctggac ggggcggtga aactgatgct gcgctatcag gtaggcaaag
     3421 agctgccgca ggaggatgtg gatgatatcg tagctttcct gcacagtctg aacggggtgt
     3481 acacgccgta tatgcaggat aaacaataat taatttgatc gcccgaacag gaatgtttgg
     3541 gcgattttta ttacgataat aaagtctgtt tttaatatta tcatgttaaa tgtttatatt
     3601 ataaaaagtc gtttttctgc ttaggatttt gttatttaaa ttaagcctgt aatgccttgc
     3661 ttccattgcg gataaatcct acttttttat tgccttcaaa taaatttaag gagttcgaaa
     3721 tggaccagaa gctgttaacg gatttccgct cagaactact cgattcacgt tttggcgcaa
     3781 aggccatttc tactatcgcg gagtcaaaac gatttccgct gcacgaaatg cgcgatgatg
     3841 tcgcattcca gattatcaat gatgaattat atcttgatgg caacgctcgt cagaacctgg
     3901 ccactttctg ccagacctgg gacgacgaaa acgtccataa attgatggat ttgtcgatca
     3961 ataaaaactg gatcgacaaa gaagaatatc cgcaatccgc agccatcgac ctgcgttgcg
     4021 taaatatggt tgccgatctg tggcatgcgc ctgcgccgaa aaatggtcag gccgttggca
     4081 ccaacaccat tggttcttcc gaggcctgta tgctcggcgg gatggcgatg aaatggcgtt
     4141 ggcgcaagcg tatggaagct gcaggcaaac caacggataa accaaacctg gtgtgcggtc
     4201 cggtacaaat ctgctggcat aaattcgccc gctactggga tgtggagctg cgtgagatcc
     4261 ctatgcgccc cggtcagttg tttatggacc cgaaacgcat gattgaagcc tgtgacgaaa
     4321 acaccatcgg cgtggtgccg actttcggcg tgacctacac cggtaactat gagttcccac
     4381 aaccgctgca cgatgcgctg gataaattcc aggccgacac cggtatcgac atcgacatgc
     4441 acatcgacgc tgccagcggt ggcttcctgg caccgttcgt cgccccggat atcgtctggg
     4501 acttccgcct gccgcgtgtg aaatcgatca gtgcttcagg ccataaattc ggtctggctc
     4561 cgctgggctg cggctgggtt atctggcgtg acgaagaagc gctgccgcag gaactggtgt
     4621 tcaacgttga ctacctgggt ggtcaaattg gtacttttgc catcaacttc tcccgcccgg
     4681 cgggtcaggt aattgcacag tactatgaat tcctgcgcct cggtcgtgaa ggctatacca
     4741 aagtacagaa cgcctcttac caggttgccg cttatctggc ggatgaaatc gccaaactgg
     4801 ggccgtatga gttcatctgt acgggtcgcc cggacgaagg catcccggcg gtttgcttca
     4861 aactgaaaga tggtgaagat ccgggataca ccctgtacga cctctctgaa cgtctgcgtc
     4921 tgcgcggctg gcaggttccg gccttcactc tcggcggtga agccaccgac atcgtggtga
     4981 tgcgcattat gtgtcgtcgc ggcttcgaaa tggactttgc tgaactgttg ctggaagact
     5041 acaaagcctc cctgaaatat ctcagcgatc acccgaaact gcagggtatt gcccagcaga
     5101 acagctttaa acacacctga taacataacg ttgtaaaaac cgaatgccca gcctttaaaa
     5161 aaacagctgg gcattcggtt gcttattaat cgcaataata tattggctgt ctattcatcg
     5221 tgttgataag atatatgtta tatgaatgtt tattaaatgt agcgattgcc tttaatgtca
     5281 cgaaaaggga ttatttgctt gctattaatt tcactgcgtt cttaaacacg tttttgtatt
     5341 ataaatccag tcatcctgcc cgtagaacat cacacattat catcctgttc tcccgcttcg
     5401 tttaaattta tttatcaatc aatttgactt aagagggcgg cgtgctacat taattaacag
     5461 taatatgttt atgtaatatt aagtcaacta tgcaatcatt acatgggaat tgtctaattg
     5521 cgtacgcaag acataaatat attctcacca tggttaatgg tgaatatcgc tattttaatg
     5581 gcggtgacct ggtttttgcg gatgcaagcc aaattcgagt agataagtgt gttgaaaatt
     5641 ttgtattagt atcaagggat acgctttcat tatttctgcc gatgctcaag gaggaggcat
     5701 taaatcttca tgcacataaa aaaatttctt cattactcgt tcatcactgt agcagagata
     5761 ttcctgtttt tcaggaagtt gcgcaactat cgcagaataa gaatcttcgc tatgcagaaa
     5821 tgctacgtaa aagagcatta atctttgcgt tgttatctgt ttttcttgag gatgagcact
     5881 ttataccgct gcttttgaac gttttgcaac cgaacatgcg aacacgagtt tgtacggtta
     5941 tcaataataa tatcgcccat gagtggacac tagcccgaat cgccagcgag ctgttgatga
     6001 gtccaagcct gttaaagaaa aaattgcgcg aagaagagac atcatattca cagttgctta
     6061 ctgagtgtag aatgcaacgt gctttgcaac ttattgttat acatggcttt tcaattaagc
     6121 gagtcgcagt gtcctgtgga tatcacagcg tgtcgtattt catttacgtc tttcgaaatt
     6181 attatgggat gacgcccaca gagtatcagg agcgatcggc gcagggattg ccgaaccgtg
     6241 actcggcggc aagtattgtt gcgcaaggaa atttttacgg cactgaccgt tctgcggaag
     6301 gaataagatt atagaatttt actcagacat aaaaaaaacc cggcataggg gaccgggaag
     6361 aggatagtct gccgtctcca gactaataaa ccgttataac actccctgtt ggcacgggaa
     6421 actttgtgct ctcagtaagt taaatataac ttttactgga aataagatca gccatttttt
     6481 tataaacata agctatacgc tgtgcgaaaa tatattcgtg ctgcatttac ttattatcaa
     6541 ttaactgtta tgcaaaacta ctttgtggat aaattttggt cctaccaaat ctggcagttt
     6601 tttcgctaag aaacagtctg gcaacatttc attagtatac tgaaattgaa ataatcgcag
     6661 tatgaaatat aagggataat catgactcat gtctgctcgg tgatcctcat tcgtcgttca
     6721 ttcgatattt atcatgaaca gcataaaata tcgctgcata acgagagtat cgtgctgctg
     6781 gagaaaaatt tggcagacga ttttgcgttt tgttcaccgg atacgcgacg actggatatc
     6841 gatgagctga cagtttgcca ttacttacaa aatattcgtc agctaccacg caatttaggg
     6901 ttacatagca aagaccgtct attaattaac cagtcacccc ccatgccgct ggtgacggcg
     6961 atttttgata gcttcaatga atccggggta aattcaccga tactgagcaa tatgctctac
     7021 ctttcctgtt tatcgatgtt ttctcataag aaagaactga tccccttact tttcaatagc
     7081 atcagcactg tttcaggaaa agttgaacgc cttattagct ttgatatcgc caaacgttgg
     7141 tatctgcgcg atatcgcgga aagaatgtat accagcgaga gtctcataaa aaaaaagttg
     7201 caggatgaaa atacctgttt cagtaaaata ttactcgctt ccaggatgtc gatggccaga
     7261 cgattactcg agttacgtca aattcctctg catactattg cagaaaaatg tggctatagc
     7321 agtacgtcgt actttataaa cacatttcga caatattatg gtgtaacgcc acatcagttt
     7381 gcgcaacatt cgccaggtac cttttcctga catattttgc atttgaatat tggtcaggat
     7441 ctcacacctg cttcatgtga aactcttccc tgatgatttc tgccgggcta ccggctagtt
     7501 ctctttcgca gtttgccagg cggtataggt attttcagga ggaaaagact cggcatgttt
     7561 gggattatta agctgacaat tcatacgatt acgggaatgt gggtcagtat tgtgctgttc
     7621 aaattgatga cgaatggctg gagtggtttc tactttcagt gctgtgtact aagcctggta
     7681 ttcctgacgg ttagctggtt gttgagtggt gaatggttag caggaaagag taaggctgaa
     7741 ccttcacgtt caaccttact ctcatttaca cgttacgctt ttttaaagcg ggcaaagaga
     7801 tgttccacta caacgaaaaa gaccggaacg aagtaaattg ccagcactgt tgcggcaaac
     7861 atcccgccca tcacgccggt acctaccgcg ttttgcgcgc cagaaccggc accatgactg
     7921 ataaccagcg gcagcacgcc gagaataaag gcgagagagg tcatcaggat tgggcgtaaa
     7981 cgcatccgcg ccgcttcgat gattgcctct atcggcgttt tcccttcttt ctgcatcatc
     8041 tcaacggcaa attcgacaat caggatggcg ttcttggcgg aaagcccgat ggtggtcagc
     8101 aaaccaacct ggaagtagac gtcattactt aagccgcgca gatcggtggc cagtaatgcg
     8161 ccaacgacgc ctaacggaac aaccaacatc accgagaacg gaattgacca gctctcatag
     8221 agtgcggcga gggcgaggaa caccacgacc agtgaaatgg catacagtgc aggagcctgg
     8281 tttgaggata acgcttcctg atacgacagt ccggtccatg agtagccgac gcctgccgga
     8341 agtttagcga ccaggtctgc cataaatttc atagcgtcac cggtactttt cccggccgcc
     8401 gcttcaccta aaatctccat tgacgggatg ccgttgtagc gttccaatcg cggtgaacca
     8461 taggtccatt cggtagacga gtaggcagaa agcggtgcca tcgtgccaga ggcgttgcgt
     8521 acataccatt ggttgatgtt atccggcaac atacggaacg gcgtgcctgc ctggacatac
     8581 acttttttca cccgcccctg gttgaggaag tcgttcacgt agctgctgcc gaaggcggtg
     8641 gaaattgtct ggttgatatc agacagcgcc acacccattg cctcagcttt cgcggcgttg
     8701 acgttcactt tgaacatcgg cgtatcttcc aggccgttcg ggcgtacccc ggtgacctgg
     8761 tcaggcgatt gcgctgccag tgataacagc tcgtttcgtg cctgggttag tttttcgtgc
     8821 cccaggttac cgttgtccag cagttccata tcaaaacctg acgcggtacc cagttcagcc
     8881 accgcgggta agttgaacgg gaagacgacg gctttattga tactgcttaa cgcaatcatt
     8941 gcccgctgaa tgatcgcggt aaccgagttt tcctcaccga cacgttcaga ccacggcttg
     9001 agactgataa acgccaggcc gttgttttgc ccctggccgc tgaagccaaa gccgccaacg
     9061 gtaaacaccg actgaacatt atctttctct tttgtaagat aataatccgt cacctgttgc
     9121 agcactttcg tggtgttaac catggtggca ccggaaggta actgcgcggt ggtcataaat
     9181 accccctgat cctcttctgg taagaaagag gtcggtgtac gcaggaacag caccgccatc
     9241 ccggcgcaaa tcagcaggta gaccaccatg tagcgaccgg tacaacgcaa cagcgagcgg
     9301 gtgctatcgg tatagtgctg agttgatttt tcaaacagcg tgttgaagcg tgcgaacagg
     9361 gcgttaggtt tgtgaccgcc ttccggcgcg gctttcagaa tggtggcgca cagggcaggg
     9421 gtcaggctca ttgccacaaa tactgaaagc agcatggagg agatcagcgt gatggagaac
     9481 tggcggtaga tctccccggt tgcgccgctc ataaaggcca tcggcataaa cactgcggaa
     9541 agaacaacag caataccgac cagcgcacgt tggatctgcc ccatcgattt atgcgtcgct
     9601 tccttcggtg gtagcttatc ttccgcaatg acacgctcga cgttctccac caccacgatg
     9661 gcgtcatcca ccagtaaccc tatcgccagc accatcccga acatagttaa cgtgttgatg
     9721 gtgaaaccga ccgccgacaa gatcgcaaac gtcccgagaa taaccaccgg tacggcaatc
     9781 gtcgggatga ttgtggcacg gaaattctgc aaaaacagat acatgaccag gaagacgagg
     9841 atgatagcct caaccagtgt tttaaaaact tcctgaatag aaatttcgat aaacggcgtg
     9901 gtgtcgtaag gataaaccgt cttcagactt gcggggaaat aggctgataa gcggttcagc
     9961 tcctctttga ctgcccgcga ggtatccagg gcgtttgctc cggtagccag tttgatggcg
    10021 atcccggcag caggtttgcc gttatagcgc gccacggtgg aataatcttc cgctccaagt
    10081 tcgacgcggg cgacatcacg cagcagcact tgcgaaccat cttgctgaac tttcaacagg
    10141 attttgccaa attcttccgg cgtttgcaga cgcgtctgca caatgatcga ggcgtttagt
    10201 tgctggtctg ccgcctgtgg catgccaccc agttgaccac cggaaatctg gttgttttgc
    10261 accttaatct gggaaataac atcggaaggt accagattgt atttattgag tttttgcggg
    10321 tccagccaga tacgcatggc gtactcggaa ccaaagagct gtacgctacc aacgcccgcg
    10381 gtacggctta gcgggtcttt gatattagac gctacatagt ccgcgatatc gtactggttg
    10441 aggctgccgt tatcagaaat aaacgccgct accatcagaa tattactgct cgacttatca
    10501 acgctaatcc cctgctgctg caccgcttca ggtaatgaag gcatagcgag ttgcagttta
    10561 ttttgcacct gaacctgtgc gatatcagga gatgtcccag tctcgaaggt cagagtgata
    10621 gaggcattac ccgccgcatc actggttgaa gacatgtaca tcaggccatc aagcccattc
    10681 atattttgct caatcacctg agtgaccgag tcttctaccg tttgcgcatc ggcacctgga
    10741 taggtagcgc tgacggtaat ggtcggtggc gcaatctgcg gatactgcgc aaccggtaag
    10801 ttcatgatcg ccagaccacc tgcaagcatc ataataatgg caagtaccca ggcaaaaacc
    10861 gggcgatcaa taaaatagtt agccatgaaa gtccccttaa gcctgcaacg ttattgtttc
    10921 gattcggtgc tggcgttttc ctggctggag gaaattgctc gtgctttgat acccggacga
    10981 atgcgttgca aaccggaaac gatcacccga tcgccagcct gcaagccaga ggtgacaacc
    11041 cactggtcgc cgatggcttt gctggcttca atttcgcgta gctgcacaac atcgtcttta
    11101 tccagaatga gcgccgttgc tttaccctgg gcgttgtgag tgacgccttc ctgcggcacc
    11161 agtaatacat tctggcggct accttcatcc actaatgccg tgacgtacat gccaggcagc
    11221 aagtcaccat ttgggttggg gaaaatcgcc cgtaacgtca cggagcccgt ggtttcatcc
    11281 accgtcgggt cggagaattt cagtgtgccg gtctggctgt agcgtttacc attttccaga
    11341 ttgagctgta ctggcgtact gccctgaacc tgtttgattt gcccactggc gacctcttct
    11401 ttcatgcgca agaaatcctg caccgactgc gtgagatcga cataaatcgg gtccagacgt
    11461 tgtacggtaa ccagcgaatc tgcctgatta gcggtaacga gtgcgccgac ggtcaccgac
    11521 gatttcccgc tgacgcccgt aatcggcgag gtgacattcg cgtattgcag attgatcgtc
    11581 gcctgttcaa cagccgcttt ggcgacggtg acattggctt ctgcttcatt cagctgggtg
    11641 cgcgcggtgt cgtaatcctg acggctaacg tagttggtct tcagcaacga tgcctggcgg
    11701 ttaaaggtga tgcgggcatt gctggcggta gagagcgctt tcgccagcga gcctttggcg
    11761 gagtttagct cggcctgtaa aggtgcagga tcaatctgat acagcgaatc gccctggttc
    11821 actttatcgc cttcgataaa gttgcgttta atgataatac cgcccacctg gggacgtatc
    11881 tcggcaactt cataaggaac ggttctaccg ggcaattcgc tcaacacatt gaccgaaccg
    11941 ggggagagtg tgacgacacc gacctcaggc gtcatggcgg cggcgttttc cgccgatttg
    12001 tcatcgcagg cggtgagcat cgcgccgcag aataacaacg gtattaacag ctttcttctt
    12061 ctgttcattt tagtccctga aaattcttga gaaataacgg ttctttaaat tgcgcgcgag
    12121 gagagatatg agggatttac agaccagcgg catagtgggg ttgtcagcca gtaaggtggg
    12181 atacaggcac agtgatcgac atggtgaggt caacgacaaa aaaaatgttg ccgttctgcc
    12241 aacagttcct gccagcattc gggcaacgaa gggttctact ggtggataca catacctggg
    12301 gaataaagtc gttttagcct ctcctttagt aatcaccggg ggtaatgaaa tcagcatctg
    12361 catccctcgt catgccagcc atcaatttca gttgcttatg tcctgactaa aaataagatg
    12421 tgatacccag ggtgacgatg tcgctcatac gtcttagttt cagcttgtac ataatgttga
    12481 atttctgaat ctttaatgtt tgttgcgaaa tattgagcgt cgacgtgata ttgctttcat
    12541 ttttattttt cagaatcagt tgcataattt tgaactgatt atgtgaaaaa cataattgtg
    12601 aattcttatg gggcaagtgt ttaccataag taatctcaag aagatttttg atactttctt
    12661 tgcggctaac aaaataaata ggcgctttag cttttagttt actgatgtga taaggtgcca
    12721 aaacaataat tctctcggca tctaatttct ccagaaattt aatcgcttct tcatcaagga
    12781 tatgattgtg ataggtatca atgataacct tgaagggttt attgcctact tttttgatct
    12841 ctgacaggga attgattttt atcatttcgt gattatcttt caactgccaa aagccctgta
    12901 aaagaaaaga atctttcgtc atgagaaaaa tcataacttg ctccttagcc gttatcgttt
    12961 cgaacatgtc atccttttca ttatttacat ccttgtccga atcgttgttc aatatagtaa
    13021 acgcctataa gaactttacc cttcgtgaac taatacgtct attaacacta atcaatactt
    13081 cagatttatc catcacgcac tccatatatt gatcctaagt aagattcctg gttgttatca
    13141 gcttgtaaat agtaaacaaa aagttaacgt ttgcggatta ctgcccaaga ataagttatc
    13201 aataaccagt ttgtgatctc tgaagaatat tactaaagtt aaaatgtaat ccgatttaaa
    13261 tatcgagtct ccttgtttcg acttaagctg gcaattggat tgccagcttt cttttaacgc
    13321 attgaaatta cctgagtaaa taacgcattc atgtcttcag gtttttatat agcgtctgga
    13381 gacacggata tttatgcaat gtaatcaacg ccagttgcaa aaaaatgaac ccggattact
    13441 tatttaaccg cccatagcat caacgcttac ggaactcttt gttcactttc ccacagcact
    13501 tttaaaacat taaacctgac tacggaaaat atcagccatg ataatgtttg aaatggctaa
    13561 tttgccatgg agtgaaaaaa attagatgaa attcagtagg ttgaaataat cactagcagg
    13621 taattatttc aatgatagtg cgcaattgat ctacaacact ccgtagcgga gagagtatta
    13681 atcggatcat agtcatatca agtgactatg atccgggtga caaccgggat aattattgct
    13741 gcttaacgaa caaactggcg aagctgaaca ggctggcggc gctaaatatc agttcaattc
    13801 ccaccagtgt ggaaaccagc gttacagaca ccatcggcgt tgcaccaagg aatatccagg
    13861 caatgacgat atccagcaca ccaataacga gctgtagcca gctgcctttc attgaacgct
    13921 gacgatacca actcatcagg cgaataaccc ctgcaacaca gaacaaaccg gcaataaatg
    13981 ccgcaatggc aaaaatgccc agctccggtg cgcggatgaa gaaatagccg atcaataaat
    14041 aggcgactgc gacgaggaaa ccggataata ccggccagaa attatgactg cggttgctga
    14101 ataacccgac aataagcgca atgcccgagc agattaataa tgcacccact actgtgctta
    14161 aaatatcgcc agagacgaac gggaaactga tacacagcaa cccgacgata aacagtagca
    14221 cggcaataaa ctggattgct ctgcgatgtt ttttaagcat ctccagatca aacttcaaaa
    14281 ttgttgcctt atctatatat aacatagaac caccctataa aattaagaag aaaatcccct
    14341 gctatcaatc tatgccaaaa acgcgtctaa gaatgtagtc gatttaataa aaatttccta
    14401 attacagtat ctgatgcatc tgtaactcat tgtattgaaa taaaaatatc tgattttgat
    14461 attttatatc aacatgacat atacagaaaa ccaggttata acctcagtgt cgaaattgat
    14521 tcgtgacggc tctttcactt tatagttgag gatattacga tgaaaaaagt attaggcgtt
    14581 attcttggtg gtctgcttct tctgccagtt gtgagcaatg cagcggatgc gcaaaaagca
    14641 gctgataaca aaaaaccggt caactcctgg acctgtgaag atttcctggc tgtggacgaa
    14701 tccttccagc caactgcagt tggttttgct gaagcgctga acaacaaaga taaaccagaa
    14761 gatgcggttt tagatgttca gggtattgca accgtaaccc cagctatcgt tcaggcttgt
    14821 actcaggata aacaagccaa ctttaaagat aaagttaaag gcgaatggga caaaattaag
    14881 aaagatatgt aattccggga atgcgttaca tcgtacttcc ttgcatattg aacaggccgg
    14941 aatatcttct ttaaaagcag ctattcctcc tgttcatata taatctctat attgaatggg
    15001 ttacaaaatg aatatttcat ctctccgtaa agcgtttatt tttatgggcg ctgtagcggc
    15061 tttgtcactg gtgaacgcac aatctgcgct ggcagccaat gaatccgcta aagatatgac
    15121 ctgccaggaa tttattgatc tgaatccaaa agcaatgacc ccggttgcat ggtggatgct
    15181 gcatgaagaa acagtatata aaggtggcga taccgttact ttaaatgaaa ccgatctcac
    15241 tcaaattcct aaagtgatcg aatactgtaa gaaaaacccg cagaaaaatt tgtatacctt
    15301 caaaaatcaa gcatctaatg acttgccgaa ttaatgaggt acaagtaaaa aggagtagca
    15361 agttgagcca tcttgctgct cctttttgca tttttatatg acagcagaat ttattatacg
    15421 tcttatactc gcggcaattg cctgtggcgc tattggcatg gaaaggcaaa tgcgcggcaa
    15481 aggagcaggg ttacgcacac atgtattaat tggcatggga agcgccctgt ttatgattgt
    15541 ttcgaaatat ggttttgctg acgtgctgtc tttagatcac gtcggactcg accccagccg
    15601 tattgctgct caggtggtga cgggcgtcgg gtttatcggt gcaggtaaca ttctcgttcg
    15661 taaccaaaat attgtaggtc tgacgacggc agcggatatc tgggtgaccg ccgccatagg
    15721 tatggttatt ggcagcggta tgtacgaact gggtatttat ggttcagtga tgaccttgtt
    15781 ggtgctggaa gtcttccatc aattaacctt ccgcctgatg aacaaaaatt accatttaca
    15841 gctgacatta gtgaacggta atatggtatc aatgctcgac tggtttaagc agcaaaaaat
    15901 aaaaactgat ttagtttcgt tgcaggaaaa tgaagatcat gaagtggtcg ccattgatat
    15961 ccagcttcat gccacaacgt cgatagaaga cctgttgcga ttattaaaag gtatggcagg
    16021 ggtgaaaggc gtttcaatta gctaatctgt gtggcagcgt agccagactc accgtaagcc
    16081 tgaaattcac accagataat caatatgctg atggcgtacc agctcattga tccgcttaag
    16141 attgaggatc atcatgatat tgtgtttatg gcagtagaac gtttttagcg atattttcag
    16201 tttctccaga atatcttcag ttgtcatttg attcgcaatg cagctcagaa cctgatgttg
    16261 cttgcgcgaa agtgacacat tcgcttcagg cacagagcag taactgccat tgatgagtgc
    16321 gacaaaatca atgatagtga tagtccgcgg aacaaaggtc accggtactt tccccagaca
    16381 gcgtttaata ttaaaggggg agaaaacaat gacatggaca ggttttaatc gttcaatttg
    16441 agtgagaaaa ttagaatgaa atacattatt cattaatgta tcaataatga cgaaggcatt
    16501 ctgatgcaac attacgtcga tctcttcttc gttctgtata tgaacgacat tacctttact
    16561 cagaatgttt ttcatcgcgg tgaagaacat cgtatccctg gtaattataa gaaacataac
    16621 cgactcctgg tgcggacatt aactttataa tcaatttgcc aaaggtaatt aacaggacag
    16681 ggcttataat aataatagtt agcttactgt atattaattt tatgtaatac atgatgcaga
    16741 tgctgagaaa gcatctgcat ctttcggtgg tgttatttga ccagctcagg tgttacctga
    16801 ctcaccgcat tggtgtagta aggcgcaccc cagcccggtt ccggccagaa tgcaccatag
    16861 ccgtaatccc acaggttata ggtggtatta accacttctc tcaaatgcca cacctggatg
    16921 ccctgcatat tcacttccag gaagttatac gggactttat tgataaagcc aggttgttca
    16981 ccctgaatgg taccgaggat ggtaacaaag tgattacgat agttcactgg gtcaaggaag
    17041 ccgctttgtc tggcgagcag tcggccctga tagttggctt caatatcagg tttcgcatag
    17101 ctatccaacg gtaatacagc gatttctaac aacgtatccg ttttgccatt gataacgttg
    17161 ataaccttcc caccaaagcg cgcttgttga ccaacatata accccggctg gttatgaaca
    17221 gcaacaaaac ttttttgaat atcaggttgg ttattgcctt tgatattttg cggaattgaa
    17281 ctacatgcgg caagcaaaaa tgaaaggctg aggatgagtg caccttttgt catgttcatg
    17341 ttactatcct tatcaacaaa tattaaaacc ataatagata tttctttata gttttcatct
    17401 gattctgagg cgatatgcct gatagtaacg taaacctgtc tgatgtttat atcccttttg
    17461 tttagttcaa ttccattgat tttggggcgt ggcatgagta tccatcgccc cataatataa
    17521 tcctttgttt taaaattaca taaaatctat tttcattcaa taattataaa gtgctaataa
    17581 tcaatattat ggtattatat tctgataatg atttgataag cgaattcttt tgccagatgg
    17641 tatttctatt tccaccccat aatttatatc tttttcatta ggaagtggcc tctttaaata
    17701 aggcgtgtgt gacatttttt tggggttaag cgtctcgtat tgaaatactt cgaactgata
    17761 ttcagacatt tctgcccatg tttgaatttt attgtatcga gagagtgtcg taaaggcttt
    17821 aacttgctta taacattgtt tttggaattc ttgctgatat tttaatatgt cattgtatga
    17881 aagattggtt atcctggcct ctaaaaattt aattattgtc gaagcaattt ccgcttttgc
    17941 tcgtaaaaag ttgtcattaa tggttaataa tgttgaaaat aaaccttcga tttgtttgtc
    18001 aagatattgt ccatctttat ataatttggg ggtagggtgt tctttatgta aaaaatagtg
    18061 tcacctaaat ttgcgtcagt gaaatttata ttaagtagat cttcgaaata aaaccttgac
    18121 aaggttagaa cgaacccgga caggttctgt cctgtcatat tgaaatcaat aaagttggct
    18181 ccggcaaaga ctttcatcct gtttttattt tcactgtgct caatatataa cttcgtcatt
    18241 ttttgataca ggctttgaat atcttttata ttagttggta agtcagctcg ttgttcagca
    18301 ataaaattag tatctgaaaa catggtttgg acactgaatg ttatgggtat tccctcttct
    18361 acgctaattt tagttatgat tgccttgtcg ggtttaggat tagcgacaac aatgattcga
    18421 cattcagcac ttttgttttt ttcatatcta atgttattaa tctgattaag tattgattgt
    18481 gtctgttgcg taagttgtgt cgaacttatc gtttcaatgt tagtaataag acttacaagt
    18541 tgagccacaa cttttgagta gtctgcattt gtatatgtag tgttcgtgtg gcacgcatta
    18601 atttgttgaa gttggtgttg cgctaaatca tattgatcag agatatatcc ttctccagag
    18661 aatgtggaat taatgggttg ttgttcttcc caattatcgc aaggccactt cggtgtgtct
    18721 ggcgttttag cattatcggt atttgttgtg cagttataaa tatcgccgtg atatgtattt
    18781 attatacctg gggtaaagtc aattgacata aaattcctta ttcatcattt gttttatgct
    18841 aagatgttta aattggataa ttagaacagg aatggatttt cttctacaca caataaatgt
    18901 aatgcgttat atcttcctga tataattacc tttgatttcc cttttatctc catccggtaa
    18961 tatttgtgta taaaaaaagg gtagtgatag tatcgtaaag gaaatcgtta acaactatca
    19021 aaatgcgctc attttaaaaa gttattatgt tctctgatag tgtgtgaagt gatttttttt
    19081 gtgatgtttt agatgtg
//
LOCUS       DAKISA010000088        18441 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.088, whole genome shotgun sequence.
ACCESSION   DAKISA010000088 DAKISA010000000
VERSION     DAKISA010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18441
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.088"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..496
                     /gene="radC"
                     /locus_tag="OVW45_003102"
     CDS             <1..496
                     /gene="radC"
                     /locus_tag="OVW45_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCV5527490.1"
                     /translation="LTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            713..949
                     /gene="rpmB"
                     /locus_tag="OVW45_003103"
     CDS             713..949
                     /gene="rpmB"
                     /locus_tag="OVW45_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCV5527491.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            970..1137
                     /gene="rpmG"
                     /locus_tag="OVW45_003104"
     CDS             970..1137
                     /gene="rpmG"
                     /locus_tag="OVW45_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCV5527492.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            1235..2044
                     /gene="mutM"
                     /locus_tag="OVW45_003105"
     CDS             1235..2044
                     /gene="mutM"
                     /locus_tag="OVW45_003105"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCV5527493.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2083..2562)
                     /gene="coaD"
                     /locus_tag="OVW45_003106"
     CDS             complement(2083..2562)
                     /gene="coaD"
                     /locus_tag="OVW45_003106"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCV5527494.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALTQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALVAKLA"
     gene            complement(2570..3847)
                     /gene="waaA"
                     /locus_tag="OVW45_003107"
     CDS             complement(2570..3847)
                     /gene="waaA"
                     /locus_tag="OVW45_003107"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCV5527495.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            4260..5318
                     /gene="rfaQ"
                     /locus_tag="OVW45_003108"
     CDS             4260..5318
                     /gene="rfaQ"
                     /locus_tag="OVW45_003108"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCV5527496.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTTPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            5315..6439
                     /locus_tag="OVW45_003109"
     CDS             5315..6439
                     /locus_tag="OVW45_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCV5527497.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            6432..7229
                     /gene="rfaP"
                     /locus_tag="OVW45_003110"
     CDS             6432..7229
                     /gene="rfaP"
                     /locus_tag="OVW45_003110"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCV5527498.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLSRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAVGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            7245..8261
                     /gene="waaO"
                     /locus_tag="OVW45_003111"
                     /gene_synonym="rfaI"
     CDS             7245..8261
                     /gene="waaO"
                     /locus_tag="OVW45_003111"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCV5527499.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAVVSRLTY
                     MDQDILNLILSGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFINAKEASPWKNEPFMRPINSNYARYCAKHNFKQNKPINGIMNYIYYFYL
                     KIIK"
     gene            8278..9273
                     /locus_tag="OVW45_003112"
     CDS             8278..9273
                     /locus_tag="OVW45_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HCV5527500.1"
                     /translation="MNEFIKERFSYLADNKKENAPELNVSYGIDKNFLYGAGVSISSV
                     LINNSDINFVFHVFTDYVDDDYLKSFNETAKQFNTSIIVYLIDPKYFADLPTSQFWSY
                     ATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPLTEIIFKDEFAAVIPDNVSTQAAC
                     AKRLNIPEMNGRYFNAGVIYVNLKKWHEANLTPYLLTLLRGETKYGSLKYLDQDALNI
                     AFNMNNIYLAKDFDTIYTLKNELHDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYP
                     SASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK"
     gene            9283..9975
                     /gene="rfaY"
                     /locus_tag="OVW45_003113"
     CDS             9283..9975
                     /gene="rfaY"
                     /locus_tag="OVW45_003113"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCV5527501.1"
                     /translation="MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLRNIND
                     TKVSLIDTKYGRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAGLTFPND
                     FYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPEDVKAEIKASMEKLHALNMLSGD
                     PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTK
                     LRKFIKKLKGKA"
     gene            10001..11026
                     /locus_tag="OVW45_003114"
     CDS             10001..11026
                     /locus_tag="OVW45_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
                     /protein_id="HCV5527502.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIKFKLNK
                     YKRKLFG"
     gene            11111..12094
                     /locus_tag="OVW45_003115"
     CDS             11111..12094
                     /locus_tag="OVW45_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCV5527503.1"
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDG
                     SKDNTSNVLREYGLTHSRLIIINKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNY
                     LLKMYTRQHETGGDAIYCGLYGHYIKDGVTYSPINTEFNEGSLLFDFFYKKVRFHIGC
                     LFIRKQLLEENNLFFDEDLRLGEDLDFIYRLLITCDMYAVPYYMYKHNYRENSLMNSC
                     RTITHYRHESFAHERIYSSVMQLYKGNRKEEIHTLLSQNRAYHKTRYLWNVLLNGDFE
                     LLNQLVESNEKELNDCNLSGKRDKRRAKILASKNYILWRMVRLVNRKKNKR"
     gene            12140..13393
                     /locus_tag="OVW45_003116"
     CDS             12140..13393
                     /locus_tag="OVW45_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCV5527504.1"
                     /translation="MSFCWNEINSGIKSLILILCIFSLMTLSLWDDVATKFLHAAGII
                     SALYFLITPKKTITNNPTLLIFISLCLLGIINIIWYSHYKVSGSVYTNAYRGPMETGK
                     IALCSAFIFLVLFAKNELRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
                     ASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFP
                     FFALLLIVMDSYINKRINYKLYCFIAIALLAGVFSFKDTLLMRMNDLNNDLVNYSHDN
                     TRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPYIDSHLHNDL
                     IDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVP
                     TAVFVTIILLCAYFKAQSDQCLLDK"
     gene            complement(13463..14434)
                     /gene="rfaC"
                     /locus_tag="OVW45_003117"
     CDS             complement(13463..14434)
                     /gene="rfaC"
                     /locus_tag="OVW45_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCV5527505.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENAAMI"
     gene            complement(14438..15484)
                     /gene="rfaF"
                     /locus_tag="OVW45_003118"
     CDS             complement(14438..15484)
                     /gene="rfaF"
                     /locus_tag="OVW45_003118"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCV5527506.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDLRVLDKEAWPLMVERYVALAYDKGVMRTAQDLPKPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            complement(15494..16426)
                     /gene="rfaD"
                     /locus_tag="OVW45_003119"
     CDS             complement(15494..16426)
                     /gene="rfaD"
                     /locus_tag="OVW45_003119"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCV5527507.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            16640..17836
                     /gene="kbl"
                     /locus_tag="OVW45_003120"
     CDS             16640..17836
                     /gene="kbl"
                     /locus_tag="OVW45_003120"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCV5527508.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANYPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            17877..18441
                     /gene="waaH"
                     /locus_tag="OVW45_003121"
                     /pseudo
     CDS             17877..18441
                     /gene="waaH"
                     /locus_tag="OVW45_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
BASE COUNT         5015 a         4001 c         4325 g         5100 t
ORIGIN      
        1 gttaacctct gaatatgaac aatttagtgg cgttcatgga attggcgtag cgaaatttgc
       61 ccagttaaag gggattgctg aactggcgcg gcgttactac aacgtgcgga tgcgtgaaga
      121 aagcccttta ctcagcccgg agatgacgcg ggaattttta caaagccagc tcacgggtga
      181 ggagcgggag atctttatgg tgatctttct cgactcacaa caccgggtta taacgcatag
      241 ccgtcttttt tccggcacgc taaaccatgt tgaagtccat cctcgggaaa ttatccggga
      301 agcgataaaa ataaacgcct cggcgctgat ccttgcacat aatcaccctt cgggttgtgc
      361 tgaacccagt aaagcggata aactcattac tgaacggata ataaagagtt gtcagttcat
      421 ggatttacgc gtgctcgacc atatcgtgat tgggcgtgga gagtatgttt cttttgccga
      481 acgcggctgg atttaacccg ctatgcgcga tccttcggga tctttgtctg ttcgggactt
      541 gagcacatcg ctgagtcagc gtatactacg ccacctttga gaatctcggg tttggcattt
      601 gggcctggca atcgagagtt cacatagaac tgcgatgacc gggctgtaaa gcctgacgag
      661 gcgccaatac cccatacgaa gctcgagcta atttgatttt tggagaatag acatgtcccg
      721 agtctgccaa gttactggca agcgtccggt gaccggtaac aaccgttccc acgcactgaa
      781 cgcgactaaa cgccgtttcc tgccgaacct gcactctcac cgtttctggg ttgagagcga
      841 aaagcgtttt gtcaccctgc gcgtatctgc taaaggtatg cgtgtaatcg ataaaaaagg
      901 catcgataca gttctggctg aactgcgtgc ccgtggcgaa aagtactaag tacttagagg
      961 aaataaatca tggctaaagg tattcgtgag aaaatcaagc tggtttcttc tgctggtact
     1021 ggtcacttct ataccactac gaagaacaaa cgtactaagc cggaaaaact ggaactgaaa
     1081 aaattcgatc cagttgttcg ccagcacgtg atctacaaag aagcgaaaat caaataattc
     1141 tcgctttgat gtaacaaaaa acctcgctcc ggcggggttt tttgttatct gcttgccccc
     1201 atattgattg catctgttca ttcctggaga tgctatgcct gaattacccg aagttgaaac
     1261 cagccgtcgc ggcatagaac cgcatctcgt tggtgcaacc attcttcatg cggtggtgcg
     1321 caacggacgc ttgcgctggc cggtttcaga agagatctac cgtttaagcg accaaccagt
     1381 gcttagcgtg cagcggcggg ctaaatatct gctgctggag ctgcctgagg gctggattat
     1441 cattcatttg gggatgtctg gcagcctgcg catccttcca gaagaacttc cccctgaaaa
     1501 gcatgaccat gtggatttgg tgatgagcaa cggcaaagtg ctgcgctaca ccgatccgcg
     1561 ccgctttggt gcctggctgt ggaccaaaga gctggaaggg cacaacgtgc tggctcatct
     1621 tggaccggag ccgcttagcg acgatttcaa tggtgagtat ctgcatcaga agtgcgcgaa
     1681 gaaaaaaacg gcgattaaac cgtggctgat ggataacaag ctggtggtag gggtagggaa
     1741 tatctatgcc agcgaatcac tgtttgcggc ggggatccat ccggatcggc tggcgtcatc
     1801 actgtcgctg gcagagtgtg aattgttagc tcgggtgatt aaagcggtgt tgctgcgttc
     1861 gattgagcag ggtggtacaa cgctgaaaga ttttctgcaa agtgatggta aaccgggcta
     1921 tttcgcgcag gaattgcagg tttacgggcg aaaaggtgag ccgtgtcggg tgtgcggtac
     1981 gccgattgtg gcgactaaac atgcgcagcg ggcaacgttt tattgtcggc agtgccagaa
     2041 gtaattcata cgcgccggat ggcataccat ccggcataaa cgctacgcta acttcgccac
     2101 cagcgcctga tggacattct ccggcaggaa atgggtgaca tcgccctgat ggcgcgccac
     2161 ctctttcacc aacgatgaag agataaacga ccactctttc gacggcatca gaaacacact
     2221 ttccagttcc ggcattaagt ggcgattcat atgcgccagc tgcatttcat attcaaaatc
     2281 cgccaccgca cgcaggccac gaatcagcac cgtagcgtgt tgattacggg cgaagttcgc
     2341 catcaaatca ctaaacccaa ccacttccac gttccccaga tgcgcggttg cctgctgtgt
     2401 cagcgccaca cgctcttcca gggtaaacat cggtttttta ctggggctgg cggcaatcgc
     2461 cagaataacg tgatcgaaca tttgcgtggc gcgcgtcacg atatcgatat gaccattggt
     2521 aatgggatcg aaagtacccg gataaatcgc ccgtttttgc ataacaacct caatgcgttt
     2581 tcggtggcag gtaaggttcc agcagttgaa gcagacgctg tagcgcgccc tggttttgat
     2641 acagtacttc aacggcatga cggccataga aactacggta atcggcgtcg gtgagtaaag
     2701 aggaaacctc ttttgcaagc gtagtggcat cggtaacggt aatcagcccg cttgcctgct
     2761 ccagccgcgc gcaaatgtct ttgaaattaa aagtatgcgg ccccatcaat accggaatag
     2821 cgtgtgcggc agcttccaga ggattatgcc ctccacgttc aaccagtgaa ccgccaacaa
     2881 aggcgagatc ggcaatgccg tacagtaaca tcaactcgcc catcgtatcg ccaaccacaa
     2941 cctgcgtgct ggtggagggg acttcccctg aagagcgtgt gatatagctt agtccagcct
     3001 ggcggacaag gttaatcgca tccgggaagc gttctggatg acggggtacc aggatgagca
     3061 ataaattcgg gaattgctgt aataatgcct gatgtgcggc gatcaccaca ctctcttcac
     3121 cttcgtgagt gctggtggca atccataccg ggcggtgtgg tgcccactgg cggcgcagcg
     3181 tcacggcttt agcagccaac tgcggcgtta cagaaatatc gaatttcagg ctaccggtaa
     3241 cggttacctg gttatttttt gcgcccagcg ccacaaaacg tgcaccatct tcttcatttt
     3301 gcgcagcaat cagcgtaata cgacgcagta agcggcggac gaatttaccc agtttggcat
     3361 aacctgcggc cgagcgggca gagagtcgcg cgttagcgat caccagcgga attttacgtt
     3421 tatgtagcgc cgcaatcagg ttaggccata gttcggtttc cataatcaac accagtttgg
     3481 ggtcgacttt attcaggaaa cggttgagcg catcgggcag gtcatacggc agataaacgt
     3541 gctgaacatc cttcccgaaa gccgattgta cgcgctccga accggttggc gtcatggttg
     3601 taacggtaat cggtaaatca ggataacgat ggcgcagcgc gcgcaccaac gggatcgccg
     3661 ccagagtttc accgacggag acggagtgca gcataatgcc gcctggtttt agcggatggc
     3721 ggtaaaaacc gtaacgttca ccccagcgtt ttcgataggc cggagcctta cgtccgcgca
     3781 cccagagccg tatccagatc agcggctgaa taaggtagag aagggcggtg taaagcaatt
     3841 cgagcatagt aaatagctga cttatggatg tgctggggat tctatgtatt tagctgtggc
     3901 tttaccatta cttttcccgt ttttgactta aatagcaatt tacccgccaa ttcttgctga
     3961 acatctatac tttttttgta tttttatgcg attcattgat ttctgctgta cgtgcaggaa
     4021 ggaatcgcag agcaacagtg aaagaatatg gttgctaatg attttatttg attgatttct
     4081 ttattgaaaa tcaacaatgt gcttaaaaag cattcgggtc ggattgccgc gaaagaagtg
     4141 atacactagc gcgtcaatat cagtgcggta tcagtattca ggtagctgtt gagcctgggg
     4201 cggtagcgtg ctttttctgc ttcaccttaa tcggataatc tcaacaaaaa gagttacttg
     4261 tggataagcc atttcgaaga attttgctca ttaagatgcg ttttcatggg gatatgttat
     4321 taactactcc cgtcattagt tcgctgaaaa aaaattaccc tgacgcaaaa atcgatgtgc
     4381 tgctttatca ggacaccacc ccgatcctgt ctgaaaatcc agagattaac gcgctctacg
     4441 gcataaaaaa taaaaaagca aaagcctcag aaaaaattgc caactttttt catctcatca
     4501 aggtattacg tgccaataag tatgacctta tcgtcaatct taccgatcaa tggatggttg
     4561 ctatactggt tcgcttatta aatgcccgtg tgaaaatttc ccaggattat catcatcggc
     4621 agtctgcttt ttggcgtaaa agtttcaccc atttagtgcc gttgcagggt ggaaatgtgg
     4681 tggaaagtaa cttatccgtg ctgacaccat tgggacttga ttcgttggtg aagcagacaa
     4741 ccatgagtta cccgcctgca agctggaaac gtatgcgtcg cgaacttgat cacgctggtg
     4801 ttggacaaaa ttatgtggtt atccaaccta cggcgcggca aatcttcaaa tgctgggaca
     4861 acgccaagtt ttccgctgtg attgatgcct tacatgctcg tggttatgaa gtcgttctga
     4921 cgtccggccc agataaagac gatctggcct gcgtcaatga aattgcgcag ggatgccaga
     4981 cgccaccagt aacggcgctg gctggaaagg tgaccttccc ggaacttggt gcgttaatcg
     5041 atcatgcgca gctgtttatt ggcgttgatt ccgcaccggc gcatattgcc gctgcagtta
     5101 atacgccgct gatatcgctg tttggtgcga cagaccatat tttctggcgt ccttggtcaa
     5161 ataacatgat tcaattctgg gcgggagatt accgggaaat gccaacgcgc gatcagcgtg
     5221 accgaaatga gatgtatctt tcggttattc cggcggcaga tgtcattgct gctgtcgata
     5281 aattactgcc ctcctccacg acaggtacgt cgttatgatc gttgcttttt gtttatataa
     5341 atattttccc tttggcggtt tgcagcgcga ttttatgcgt attgctcaga cagtcgccgc
     5401 ccgaggtcat catgttcggg tttataccca gtcgtgggaa ggcgaatgcc ctgatgtatt
     5461 tgaactgatc aaagtgccgg ttaaatcgca taccaatcac gggcgcaatg cggagtattt
     5521 tgcctgggtg caaaaacatt tacgcgaaca tcccgtcgat aaagtcgttg gattcaacaa
     5581 aatgccgggg ctggacgttt attatgccgc tgatgtttgt tatgccgaga aagtcgcgca
     5641 ggaaaaaggc tttttctatc acctgacgtc acgttatcgc cattatgccg cctttgagcg
     5701 ggcaaccttc gaacagggca agccgacaca gctgctgatg ctgacagata agcaaatcgc
     5761 cgatttccag aaacattatc agactgaagc ggagcgtttt catattctgc caccggggat
     5821 ttatcctgat cgtaaatata gccagcagcc agctaatagc cgtgaaatct tccgtaagaa
     5881 gaatggaata accgaacaac aatatttatt gttgcaggtc ggttcagact tcacgcgtaa
     5941 aggtgtcgat cgttccattg aagcacttgc ttcgttaccg gattcgctgc gccacaacac
     6001 attgctatat gttgttggac aggataagcc gcgaaaattt gaggcgctgg cagaaaaacg
     6061 cggcgtgcgc agtaatgttc acttcttctc ggggcgcaac gatgtctcgg aattaatggc
     6121 ggcggcggat ttattactgc atcctgccta ccaggaagca gcgggaattg tgctactgga
     6181 agcgattact gcaggattac cggtactaac aacagccgtt tgtggctatg cgcattatat
     6241 tgtcgacgcc aattgcggcg aggctattgc tgagccattc cgccaggaaa cattgaatga
     6301 gattttacgc aaagcgttaa cgcaatcttc attgcgccag gcctgggcgg aaaatgcgcg
     6361 acattatgct gatacacaag atttatacag tctgccagag aaagcggcgg atatcataac
     6421 gggtggtctg gatggttgaa cttaaagagc cgtttgccac gttatggcgc ggtaaagatc
     6481 cttttgagga agttaaaacc ttgcagggtg aggtttttcg tgaactggaa actcgccgca
     6541 ctctgcgatt tgaaatggcg ggcaaaagct attttctcaa atggcatcgc ggcacgaccc
     6601 tgaaagagat aatcaaaaat ttactctcat tgcggatgcc agtattaggc gcagaccgcg
     6661 aatggaatgc gattcatcga ctgcgggatg tcggcgttga tactatgtat ggggtggcat
     6721 ttggcgaaaa aggtattaat ccgctgagca gaacctcgtt tattattacc gaagatctga
     6781 ccccaaccat cagtctggaa gattactgtg ctgactgggc gaccaaccca ccagatgttc
     6841 gcgtaaagcg tatgcttatt aagcgtgtcg cgacgatggt gcgcgatatg catgctgtgg
     6901 gcattaacca ccgtgactgt tatatctgtc atttcctgct gcacttgcct ttttccggta
     6961 aggaagagga gttaaaaatt tcggtaattg acctgcaccg ggcgcagctt cgcacgcgcg
     7021 ttccacgtcg ttggcgcgat aaagatctta ttgggcttta tttttcttcg atgaatatcg
     7081 gcctgactca gcgggatatc tggcggttta tgaaagtgta ttttgtcgcc ccgcttaaag
     7141 acattctcaa gcaggaacaa ggactgctgt cgcaagcaga agcaaaagcc acaaaaatca
     7201 gggaaagaac gattcgaaaa tcgttgtaat ttccggagag aaatatgagt gcccactatt
     7261 ttaatccaca agagatgatc aataagacaa tcatcttcga tgaaaggcca gcggcgtcag
     7321 tggcatcatc attccatgtt gcttatggca ttgataaaaa ctttcttttt ggttgtggtg
     7381 tttcaatcac gtcagttttg ttacataaca acgacgtgag ttttgttttc cacgttttta
     7441 ttgatgatat ccctgaagcc gatatccagc gtttagccca attggcgaaa agctatcgta
     7501 cctgtatcca gatccatctg gtaaattgtg aacggcttaa ggcattaccg acgaccaaaa
     7561 attggtctat tgccatgtat ttccgttttg taattgcaga ttactttatt gatcaacaag
     7621 ataagatcct ttacctggat gctgatatcg cctgtcaggg aaacttaaag ccgctgataa
     7681 caatggatct tgccaataac gttgctgctg ttgttactga acgcgatgct aactggtggt
     7741 cgttacgggg tcaaagtctg cagtgtaatg aacttgaaaa gggttacttt aattcaggtg
     7801 tcctgttaat taatacacta gcgtgggcgc aggagtccgt ttctgctaaa gcgatgtcga
     7861 tgcttgctga taaagccgtc gtttcccgtt taacctatat ggatcaagat atacttaatc
     7921 ttatcctgtc agggaaagtt aaattcattg atgctaaata caatactcaa tttagtttaa
     7981 attatgaatt aaaaaaatca tttgtttgtc caattaatga tgaaaccgta ttaattcatt
     8041 atgtcggccc gacaaaaccc tggcattact gggccggtta tccaagtgcg caacctttta
     8101 tcaacgccaa agaagcatcg ccctggaaaa atgaaccgtt tatgcggcca attaattcaa
     8161 actatgctcg ttattgcgcc aagcataatt ttaaacaaaa taaaccaatt aacgggataa
     8221 tgaattatat ttattatttt tatttaaaga taataaaatg atattgagaa aagttaaatg
     8281 aatgaattta taaaagaacg gttttcgtat ttagcagata ataaaaaaga aaacgcccca
     8341 gaactaaatg tttcctacgg tatcgataag aattttttgt atggtgctgg cgtttcaatt
     8401 tcttccgttt tgattaataa ttcagatatt aattttgtct ttcatgtttt cactgattat
     8461 gtggatgatg attatttaaa gtcatttaat gaaacagcaa aacaatttaa tacctcaatt
     8521 attgtatatt taattgaccc aaaatacttt gctgatctgc cgacgtcaca gttttggtcg
     8581 tacgcgacat acttcagggt attgtccttt gaatatctga gtgaaagtat ttccacactg
     8641 ctgtatctgg atgccgatgt cgtttgtaaa ggaagtctga agcctctcac agaaattata
     8701 tttaaagatg agtttgctgc ggtcattcct gacaatgtta gtactcaggc ggcatgtgca
     8761 aaacgcctca acattcccga gatgaatgga cgttatttca atgcaggcgt tatctatgtc
     8821 aatcttaaaa aatggcatga agcaaatttg acaccgtatt tactcacgct tttacgaggg
     8881 gaaactaaat atggctctct taaatattta gatcaggatg cgttgaatat cgcatttaat
     8941 atgaataata tctacctcgc gaaggatttt gatactattt ataccctgaa aaacgaactt
     9001 catgatcgta gtcatcgaaa gtatcagcaa accattaccg ataaaacagt attgattcac
     9061 tatacaggga taactaaacc atggcatagc tgggctggtt atccgtctgc atcatacttt
     9121 aatatcgcgc gtgaacaatc tccctggaag aaatatcctc ttaaagaggc gcggactgtt
     9181 gcagaaatgc agaaacaata taagcatctg tttgcccatg gtgagtatat taaaggcata
     9241 acttcattaa ttaagtacaa gcttaagaaa taaattactc ttatgattac aagtatacgc
     9301 tatcgcggct tctcatttta ttacaaagat aatgataata aatataaaga aatctttgat
     9361 gaaatactgg cctataattt taaaacagta aaagtattac gtaatattaa tgataccaaa
     9421 gtatcattga ttgatacgaa atatggtcgc tatgttttta aagtctttgc cccgaaaacg
     9481 aaaaggaatg aacgtttttt aaaatctttt gtaaaaggtg actactatca gaatttgatc
     9541 gttgaaaccg atcgtgtcag aagtgcaggt cttacttttc ctaatgattt ttattttctg
     9601 gctgagcgta aaattttcaa ctatgccagt gtttttatca tgcttatcga gtatgtcgag
     9661 ggcgtagagc tcaatgatat gccgatcatt ccagaagatg ttaaagcaga aattaaggct
     9721 tcaatggaaa aattgcatgc cttaaatatg ctatcgggcg acccacaccg gggtaacttt
     9781 atcgttagta aagatggtgt caggataatt gacctatccg gtaaaagctg tacggcagag
     9841 cgtaaagctc gtgatcgtct ggccatggaa cgtcacctgg gtattgccaa cgaaataaaa
     9901 gattatggct actattcagt aatctacaga accaaactac gcaaatttat taaaaaatta
     9961 aaaggcaaag cgtaaaccac acagtcaaaa cggaaccaac atggatttat tagctgagag
    10021 tattactgaa gtcgctgtct ctggggaaat tgctaacacc gatcgtgtgt taaatatcgc
    10081 ttacggtatt gaccgtaact ttttatttgg tgcggcagta tctatgcaat cagttgttat
    10141 gcataatccg gaccttgcgg ttaagtttca tctctttacc gactacattg atgaagatta
    10201 tctacaacgt gttaatgctt ttaccagcaa aaatgctaac gttgaagtaa gaatttataa
    10261 agtctccagt gcctttattg atatcttccc cagcctgaaa cagtggtctt atgcaacatt
    10321 cttccgttta gttgcgttcc agtatctgag tgaaactatt gaaaatctgt tatatatcga
    10381 tgctgatgtc atctgtaaag gctcattagc tggattgctt gatattaatt ttgatgggga
    10441 taagtttgca gctgttatta aagatgtgcc ttttatgcag gaaaaaccag cgaagcgtct
    10501 ggctatagag ggacttccag ggaattattt caacgccggt gtagtatatc tgcagcttga
    10561 agcatgggcg aaaaatgatt ttatgaataa agccattgct atgctggcaa gtgacccgca
    10621 gcacacgaag tataaatgcc ttgatcagga tattttaaat attctgttct ttggtcattg
    10681 tatttttatt agcggcgatt atgattgctt ttatggcatc gattatgagt taaaaaataa
    10741 aagcgatgaa gattataaaa agaccattac cgatgatact aagctgattc attatgttgg
    10801 cgtaacgaag ccctggaacg actggacgaa ttatccctgc cagaagtatt ttaatgaggc
    10861 ttatcaggct tcttgctgga atgatgtggc gtttattcca gccacgaatg aaaagcagta
    10921 tcaagtgaaa tatcaacatg caaagaaaaa tggtgatacg tttaacgctt ttatttactt
    10981 tattaaattt aaattaaata agtataaaag aaaactattt ggataataat aaagatcatg
    11041 gatgtctgag ttacaataat aattaaggat gagtactatc atattttatt agttcaggca
    11101 agagaacacg atgagcaatg attacccttt agtatccata ataataccga cgtataattc
    11161 atctgattac attactgaaa ctctaacgaa attagaaaaa caaacttacc caaattttga
    11221 aattgttatt gttaatgatg gttctaaaga taacacatca aacgttttga gagagtatgg
    11281 gttaacccac tctcgattaa ttattatcaa taaagaaaat ggcggtgttt cgtctgccag
    11341 gaatacaggt atccgcaagg cgcaaggaca gtttatatgt tttatggatg atgatgatga
    11401 gatagatcct aactatctgc tgaagatgta taccagacaa catgagacgg gaggggatgc
    11461 gatttattgt gggctttatg gccattatat aaaagatggt gttacttact cacctataaa
    11521 tacagagttt aatgaaggat ctttactttt cgactttttt tataaaaagg ttagattcca
    11581 tatagggtgc ttgtttataa gaaaacaact tctggaagag aataatcttt tttttgatga
    11641 agatttacga ctaggagaag atctggattt tatctatcga ctgctaatta catgcgatat
    11701 gtatgcggtt ccatattata tgtataagca taactataga gaaaattcct taatgaactc
    11761 atgtagaacc atcactcatt atcgacatga gtcatttgcg cacgaaagaa tctactcttc
    11821 tgtgatgcag ttatacaaag gtaacaggaa agaagaaatt catacattat tgagtcaaaa
    11881 tagagcttat cataaaactc gttatttatg gaatgttcta cttaatggtg attttgaact
    11941 attaaatcaa ttagttgaaa gcaatgaaaa agaattgaat gattgtaatc tttctggcaa
    12001 gagagataaa agacgagcaa aaatattagc atcaaaaaat tatattctct ggaggatggt
    12061 aagactggta aataggaaaa agaataaacg ttagatcttg cattaaataa tccgtcctcg
    12121 tagcataggt tgaaactata tgtcattttg ttggaatgaa attaattctg gtataaagtc
    12181 tttaattctc atattatgta ttttttcttt gatgactttg tctttatggg atgatgttgc
    12241 aacaaagttt cttcatgcag ctggaattat atctgcattg tattttctaa taacaccaaa
    12301 aaaaacaata actaacaatc ctactttgtt aattttcatc tcattatgtc ttttgggaat
    12361 cataaatatc atctggtatt cacattataa agtttcaggc tctgtttata ccaatgcata
    12421 tcgtggccca atggaaactg ggaaaattgc cttgtgtagt gcttttattt tcttggttct
    12481 ttttgctaaa aatgagttga gaacaaaaat aaaatttggg aaactaattc tgttcgcatc
    12541 cttggcaacg cagttacttt tttttgcgca tgccatgtgg caacatttct atttaaacgt
    12601 tgaccgtgtt gcattatcag cttcccacgc tacaacagca ggctacatca tccttttccc
    12661 ttctttactg gcatcaattc tcattttaaa atccgacttt agacataaaa caacattata
    12721 tacaattaac ttcatgctta gcttatgtgc tgtcatagta actgaaacgc gtgcagccat
    12781 attagtgttt ccattctttg cgttactatt aatcgtaatg gatagttata ttaataagcg
    12841 aattaattat aagttatatt gttttattgc gattgcatta ttagcaggtg tattttcttt
    12901 taaagataca ttgcttatga gaatgaatga cttaaataac gatttagtta attattcgca
    12961 tgataacacc agaacttcag tcggtgcccg tctggcaatg tatgaagttg gcttaaaaac
    13021 atattctcca ataggacaat cactggaaaa acgagcggaa aaaatacatg aactagaaga
    13081 aaaagagcct agattgagtg gcgctttacc ctatatagat tctcatttgc ataacgatct
    13141 catagatacg ttatcaacgc gtggtattcc tggagttgta ttaacaattt tagccttttc
    13201 agcaatactc atatatgcct taagaactgc taaagaacct tatattttaa tcttgctttt
    13261 ttcactactg gtagtaggcc taagtgatgt aatactcttt tctaaaccgg ttccgactgc
    13321 tgtgtttgtc accataatat tgctttgtgc ttattttaaa gcacaatcag accaatgttt
    13381 attagataaa taatgaatat gtgaaataaa atcagcaagg aggctgattt tatttcaaaa
    13441 ttatctatta aggggttgta atttaaatca tagcagcgtt ttcttcaatg aatcgcttaa
    13501 cagcatttgc tgtcaattga gacaactcat tccccggagc cctacaaacc atctgattct
    13561 tcccatatcc accaattaat cccggatcgg ttggtccata aaccgtgata ttgggtctat
    13621 ccagtgccgc cgttaaatgg cttaaccccg tatccaccga cactacaaat ttagccccgg
    13681 ccagcacgcg ggcaacgcct tccagactca tcttcggcaa tacttcaaca taagcaaatc
    13741 cttccgccag tcgtttcgcc cgctcttcct catgcggcgc gccccacgga agtttaatcc
    13801 gtattcctga atcagccagt aaaccaatca attctcgcca gtgttcttcc ggccagtgtt
    13861 tatcatcacg ggtcgtcgca tgaagaaata cggcatattc gccagcatct gtaggcaggt
    13921 tcgtcaggaa atgctgtgca atagcataat cgccctgggt ttgcggtttg ctatagccca
    13981 gacttttggc aaacagttcg cgggtgcgtt ctacggcgtg ctgctgtttt gcaatatgat
    14041 gcttacgatt gtaaaacaga ctggctaaag gttcgcgagc ggtttgccag tccatgccat
    14101 gctttacgcc atgcgccaga cgcgtcacca gcgccgcgct ttttaccagc ccctgagcgt
    14161 cgataactgc gtcatagttc tctgcttgta gcgcttcacg aaacgctttg cgttccgctt
    14221 ttatgggggc cgagaaccag gctttacgcc agcgacgtat tgccacagga ataactcgct
    14281 caacggcggc gtgccaggaa ggaatctgtg cgaacccttc ttccaccacc cagtcaaact
    14341 taatccctgg gattgcctgc tgggcatcag tgagtgcggg caacgtatgg agaacatcgc
    14401 ccatcgacga tgttttaacg atcaaaaccc gcatccgtca ggcttcctct tgtaacaata
    14461 gcgcgttgag ttcttccagt acgcgctggg gagtaatgtc gattaagctc tggtgataac
    14521 cctccgcagc gtcaccttta cgcactttgt gatagccggt aatcaggcgg atcacgcgcg
    14581 ctttatggga tagcggcggt gtgaagtccg ggctactcgg accatacagg gcaaccagcg
    14641 gacgattgag cgccgccgca acgtgcatca ggccagaatc gttagtgaca atggctttac
    14701 aggctgcaat caggataacc gcttgatcaa gctgtgtttc ccccgccagg ttccgacacc
    14761 atgcctgctg ctcggtattc aaagcggcaa gaatctcatt gcccgcttca tgatctttcg
    14821 ccgagccaaa cagaaccacc tgataacctt catcaatcag ctgctttgcc agttccgcat
    14881 agtggtagtg tggccagcgt tttgccggac caaactccgc acccgggcaa aaaccaatca
    14941 tcggacgttc tgatgaaagc gaaaattgat tacaggtata tgatttttca ccttcgctca
    15001 cctgcaattg cggccataac aacggttttg gtagatcttg tgcggtacgc atcacgcctt
    15061 tgtcataggc cagcgcgaca tagcgttcca ccattagcgg ccaggcttct ttatcgagca
    15121 cgcgtaaatc gttgagtaaa ccgtagcgca tctcgccgcg ccagccggtg cgatgaggaa
    15181 tacccgcgaa gaaaggcact aatgcagatt tgaaggagtt gggtaagacg taggcgcggt
    15241 cgtagcgctt ttcacgcagg ctatgaccca gtttgcggcg ttcgccgatt tccagcgctc
    15301 cgtgaccgag aggcatagga atagcttcgt taacttccgg catccgcgat aataatggac
    15361 ggcaccatgc cggtgccatc acatcgatta tcgcctgggg atagcgcgcc tggagcgtgc
    15421 gatagagact ttgcgacatc atcatgtcgc caacccaaga cgggccgatc accagtattt
    15481 tcatgcagag ctcttatgcg tcgcgattca gccaggccat gtattccgtt acaccttcag
    15541 caacggtttt gaacggtttg tcgtaacccg ccgcgcgcag atttgtcaga tctgcctgag
    15601 tgaacgcctg gtagcggcct ttcagcttat ccgggaacgg aatgtattcg atctggcctt
    15661 tcttgtgata agccagcgta gcatcagcta cagcctggaa ggattccgca cgaccagtac
    15721 cgaggttgaa gatgccggaa acgccatttt ccaggaacca cagattcaca tcagccacgt
    15781 cgcccacata gacgaagtcg cgtttgaagt tctcgctacc ttcaaacagc ttcggtgatt
    15841 caccgttgtt aagctgggtg ttgagatgga aagcgacgct cgccatgctg cctttatggc
    15901 cttcacgcgg tccataaacg ttgaaatagc ggaagccaac aatctgcgag ttagcttcag
    15961 gcaggatttg gcgaacatat tcatcaaaca ggaattttga gtaaccgtag acgttcaacg
    16021 gtttttcgta ctcgcgggat tcaataaagt cggaggtgcg tccgccgtag gtggctgcgg
    16081 aagaggcata caggaacggg atttcgcgct ccagacagta gtgcagcagc tctttggagt
    16141 attgatagtt gttatccatc atatacttgc catcccactc ggtggtggaa gagcacgcgc
    16201 cttcgtggaa aatcgcttcg acatcgccga actcttcgcc agccataatc tggatcagga
    16261 agtcttcctt atccatatag tccgcgatat ccagatccac caggttcaca aacttggtgc
    16321 cgtctttcag gttgtccacc accagaatat cggtgatgcc tttatcattc agggccttaa
    16381 cgatgttgct gccgataaag cccgcgccgc cggtaacgat gatcataact gtaaccttcg
    16441 aattatggag tcagagacaa tctcagacac gaatacttct atcatatcac tacacggcgt
    16501 tggcttcagc cattcgccaa aagtgagatt gatggacgtt atttatcctg aattttgcag
    16561 aagtgttaac gcgtcatctc gtcgcgacct ataagtttgg gtaatatgtg ctggaatttg
    16621 ccctgtctgg agaatcgcaa tgcgtggaga attttatcag cagttaacca acgatctgga
    16681 aaccgcacgg gcggaagggt tgtttaaaga agagcgcatt attacgtctg cgcagcaagc
    16741 agatatcact gtggctgatg gaagccacgt cattaacttt tgtgccaata actatctcgg
    16801 gctggcgaat tatccggatc tgattgcggc ggcaaaggcg ggaatggatt ctcacggttt
    16861 cggcatggct tcggtgcgtt ttatttgcgg cactcaggac agccataaag agcttgaaca
    16921 aaaactggcg gcattcctgg ggatggaaga tgcgattctc tactcttcct gctttgatgc
    16981 taacggtggc ctgtttgaaa cgctgctggg cgcggaagac gccattatct ccgacgcact
    17041 gaaccacgcg tctattattg atggtgtgcg tctgtgtaaa gctaaacgct atcgctatgc
    17101 caacaacgat atgcaggagc tggaagcacg tctgaaagaa gcgcgtgaag ccggtgcgcg
    17161 tcatgtgctg attgccaccg atggtgtgtt ctcgatggac ggcgtgattg ccaacctgaa
    17221 gggcgtttgc gatctcgcag ataaatatga tgccctggtg atggtagacg actcccacgc
    17281 ggtcggtttt gtcggtgaaa atggtcgtgg ttcccatgaa tactgcgatg tgatgggccg
    17341 ggtcgatatt atcaccggca cgctcggtaa agcgctgggc ggggcttctg gtggttatac
    17401 cgcggcgcgc aaagaagtgg ttgagtggct gcgccagcgt tctcgtccgt acctgttttc
    17461 caactcgctg gcaccggcca ttgttgccgc atccatcaaa gtactggaga tggtcgaagc
    17521 gggtagcgag ctgcgtgacc gtctgtgggc gaacgcgcgt cagttccgtg agcaaatgtc
    17581 ggcggcgggc tttaccctgg cgggagccga tcacgccatt attccggtca tgcttggtga
    17641 tgcggtagtg gcgcagaaat ttgcccgtga gctgcaaaaa gagggcattt acgtcaccgg
    17701 tttcttctat ccggtcgttc cgaaaggtca ggcgcgtatt cgtacccaga tgtctgcggc
    17761 gcatacccct gagcaaatta cgcgtgcagt agaagcattc acgcgtattg gtaaacaact
    17821 gggcgttatt gcctgaggat gtgagatgaa agcgttatcc aaactgaaag cggaagttag
    17881 aggacgacct cgacgtggcg cagtgcaacg ctgactggtg ttttcgtgaa acgggagaaa
    17941 cctggaaatc catccccagc gatcgccttc gctcaaccgg cgtattaacc ggcccggact
    18001 ggctgcggat ggggctttct tcgcgccgtt ggactcacgt tgtctggatg ggggtttatc
    18061 gccgcgatgt tattgttaaa aataacatta aatttattgc cggattacat catcaggata
    18121 ttgtctggac aacagaattt atgtttaatg cgctgcgtgc gcgatatacc gagcaatcgt
    18181 tatataaata ttatctgcat aatacgtcag tgagtcggtt gcatagacaa gggaacaaaa
    18241 accttaatta tcaacgtcac tatattaaga ttactcgtct gctagagaaa ttaaatcgaa
    18301 attatgccga caaaattacg atttatccgg aatttcatca gcaaataact tacgaagcat
    18361 tgcgtgtttg ccatgcggtg cgcaaagagc cggatattat tacccgccaa cggatgattg
    18421 ccgagatatt tacttccggt a
//
LOCUS       DAKISA010000089        18163 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.089, whole genome shotgun sequence.
ACCESSION   DAKISA010000089 DAKISA010000000
VERSION     DAKISA010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18163
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.089"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..760
                     /locus_tag="OVW45_003122"
     CDS             <1..760
                     /locus_tag="OVW45_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HCV5527509.1"
                     /translation="RKHPDSNQFNTRYKYSPTHEEAAVCCVPNTARTIPAFVENMFQE
                     NNNGFTALFYGPCEFYGTFNNVAIKITQLTEYPHDLSVKCLIEPESSVRFALSFRYPG
                     WAKMMIINGVTFTKNDTQNSLIILDRTWQHHDVIDIKFIADIQFNTDLCGDTYISRGP
                     VLYAIELESDILIKKTLLNGKYFDSGYIPCSRADESIQFSYADMESFSYSTSPEGKQY
                     IEGCFYLNKTKIVRKLIPFGQTILRKVTFSNIES"
     gene            797..3871
                     /locus_tag="OVW45_003123"
                     /pseudo
     CDS             797..3871
                     /locus_tag="OVW45_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
     gene            complement(3959..4930)
                     /gene="lpxM"
                     /locus_tag="OVW45_003124"
                     /gene_synonym="msbB"
     CDS             complement(3959..4930)
                     /gene="lpxM"
                     /locus_tag="OVW45_003124"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCV5527510.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(5050..6372)
                     /gene="mepM"
                     /locus_tag="OVW45_003125"
     CDS             complement(5050..6372)
                     /gene="mepM"
                     /locus_tag="OVW45_003125"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCV5527511.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAAHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(6388..7320)
                     /gene="znuA"
                     /locus_tag="OVW45_003126"
     CDS             complement(6388..7320)
                     /gene="znuA"
                     /locus_tag="OVW45_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCV5527512.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            7399..8154
                     /gene="znuC"
                     /locus_tag="OVW45_003127"
     CDS             7399..8154
                     /gene="znuC"
                     /locus_tag="OVW45_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCV5527513.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            8151..8936
                     /gene="znuB"
                     /locus_tag="OVW45_003128"
     CDS             8151..8936
                     /gene="znuB"
                     /locus_tag="OVW45_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCV5527514.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(9083..10093)
                     /gene="ruvB"
                     /locus_tag="OVW45_003129"
     CDS             complement(9083..10093)
                     /gene="ruvB"
                     /locus_tag="OVW45_003129"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCV5527515.1"
                     /translation="MIEADRLISAGTTLPEDGADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(10102..10713)
                     /gene="ruvA"
                     /locus_tag="OVW45_003130"
     CDS             complement(10102..10713)
                     /gene="ruvA"
                     /locus_tag="OVW45_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCV5527516.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARTDASSETLIREALRAAL"
     gene            complement(10852..10917)
                     /gene="yobI"
                     /locus_tag="OVW45_003131"
     CDS             complement(10852..10917)
                     /gene="yobI"
                     /locus_tag="OVW45_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCV5527517.1"
                     /translation="MYIFITHLFTEYVILKYLLPI"
     gene            10990..11592
                     /locus_tag="OVW45_003132"
     CDS             10990..11592
                     /locus_tag="OVW45_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCV5527518.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(11594..12115)
                     /gene="ruvC"
                     /locus_tag="OVW45_003133"
     CDS             complement(11594..12115)
                     /gene="ruvC"
                     /locus_tag="OVW45_003133"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCV5527519.1"
                     /translation="MAIILGIDPGSRVIGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(12150..12890)
                     /locus_tag="OVW45_003134"
     CDS             complement(12150..12890)
                     /locus_tag="OVW45_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCV5527520.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(12919..13371)
                     /gene="nudB"
                     /locus_tag="OVW45_003135"
     CDS             complement(12919..13371)
                     /gene="nudB"
                     /locus_tag="OVW45_003135"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCV5527521.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQVVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(13489..15261)
                     /gene="aspS"
                     /locus_tag="OVW45_003136"
     CDS             complement(13489..15261)
                     /gene="aspS"
                     /locus_tag="OVW45_003136"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCV5527522.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTVLAELSIQVVKKAENN"
     gene            15571..16137
                     /locus_tag="OVW45_003137"
     CDS             15571..16137
                     /locus_tag="OVW45_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCV5527523.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            16492..16740
                     /locus_tag="OVW45_003138"
     CDS             16492..16740
                     /locus_tag="OVW45_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCV5527524.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            17341..>18163
                     /locus_tag="OVW45_003139"
     CDS             17341..>18163
                     /locus_tag="OVW45_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCV5527525.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGP"
BASE COUNT         4758 a         4453 c         4079 g         4873 t
ORIGIN      
        1 aaggaaacat cctgacagta atcagtttaa taccagatat aaatactccc ctactcatga
       61 ggaggctgct gtctgctgcg taccgaatac agcaagaacc ataccggctt ttgtcgaaaa
      121 tatgtttcaa gaaaataaca atggttttac tgcattattt tatggcccgt gtgagtttta
      181 tggcacattt aataatgttg caattaaaat tacacaatta actgaatacc ctcatgattt
      241 aagcgtaaaa tgtctcattg agcctgagtc atctgttcgt tttgctctca gtttccgtta
      301 ccccggctgg gcaaaaatga tgattattaa tggtgttact tttactaaaa atgatactca
      361 aaactcactt attattttag atagaacatg gcaacatcat gatgtgattg acattaaatt
      421 tatagctgat atacagttta atactgactt atgtggtgac acttatattt ccagaggtcc
      481 cgttttatat gcaattgagc ttgagagcga tattttaatc aaaaaaaccc tgctgaacgg
      541 gaaatatttt gactcaggat acataccctg tagtagagct gatgaatcta tccagttctc
      601 ttatgctgat atggaatctt tttcatattc aacctcacca gagggcaaac aatacattga
      661 aggctgtttt tatttaaaca agactaaaat agtcagaaaa cttataccat ttggtcaaac
      721 cattcttcgc aaagtaactt tctccaatat agagagttaa tattttcgag tgttataatt
      781 aattaaaggg atttctatgt accttactga taatattatc aaactgtcga ataaaatagg
      841 tcagcgtgtt tatactgttg tctctgaaat gtcgattgaa gcatggataa caaaagaacc
      901 gcagcctttt gtttatcgcc gtagcggcgc ttaccagaaa ctcaccattg gctcagactg
      961 gggtcaactg tttgactgtg cctggatgcg agtttatggt aaacgtccag cagataaatt
     1021 tagacacaaa ctcgtcgctc tcgttgatat cggtggcgaa ggtcttattg tggataagaa
     1081 tggttcaccc atctgcggca tcacgaataa agcatcatct tatggcgtac cgccagataa
     1141 accaggtaaa tgggtggttg acctttctct cgttagtgaa aatgacgagg ttgagttttg
     1201 gattgatgct gcctgcaacg atttatttgg ctatgttact aatggaggga ttattactga
     1261 tgttcatatc gcgacacgta atcaactact gaaatccctt tattatgata ttgaggtgct
     1321 tttcgactgg attaatgatg gtcagaagtt tgaatccata catcctaaag gtatcacccc
     1381 agaacaattt attgccaaaa gaagtgagcg tactgacgaa atcataagta ctctcgaata
     1441 tattgataat acgttaatta cctttagcaa tgaagaaata attaaatgtc aaattgcaat
     1501 ccaatccata atcaataaaa acaataatcc ttcagagttc aggattatgg ctactggtca
     1561 tgcccattta gatatagcgt ggatgtggcc tttaagagaa ggacgcagaa aagcaataag
     1621 aacatttgcc accgcccttg caaacattga aaaatatcct gattacattt ttggcgctag
     1681 tcagtaccaa ctttttcact ggataaaaaa agattatccc tatttcttcg aaaagttaaa
     1741 aaaacaaatc gccgcaggcc gctttgagtt acaaggttgc ttctgggtcg aatgcgattt
     1801 aaatctggta agtggtgaat cgctgataag gcaaatcatg catgggacac gattcacact
     1861 gcagaatttt tcaaaaaaga taaattatgt ctggcaaccg gatgtctttg gattccccgc
     1921 aacgcttcca caaattctca aaaaatcagg cattaactat attgcatcgc aaaagctctc
     1981 acaaaacaag attaataaat ttaataatta ccttttccgc tggcagggcc tggatggctc
     2041 agaaattctg atgcataact ttcctgaaga cacttatgat agccgagcac gtgctcgctc
     2101 tttagaatat attgaacaaa actataatga aaaagagatc tgtccctatg cgcttatggt
     2161 gtatggtgtc ggtgatggcg gcgctggccc cggtgaggaa catatagaac gattaacacg
     2221 tatacgcaat attgatggct tacctcatgt tgatttcagc cgtgttgata agttcttcac
     2281 ttacgccgac gcctttcgcg aaagcttgcc cattatttca ggtgaacttt atttcgaggc
     2341 tcatcaaggg tgctttacat cagagtcagc aactaaagct cataatcgaa atatggaaaa
     2401 taaattacat gatgctgaat tttttataac tattactaat aatatgacat caattcttcg
     2461 cagtgagttt gatgaaatat ggaaagcgat gctgaccttg caattccatg acatccttcc
     2521 cggctcatgt atttcaagag tttatcatga aactgaaaag gaatatctta aactagaagc
     2581 aaaaaccgag aaaattattt ctgatgcgca gtctacttta ttaagtaaaa ttgacacttc
     2641 ttcttataaa gatccgcaca ttttgtttaa taccacctgt tttgctcgta acgaatggat
     2701 caatataaac aacaactggc tcaaagcacg agtaaactct tatggttatg ctgtgattga
     2761 tccaaagaat aagatcgtaa atggtctgaa ggcagaatct caaagtattg aaaataatta
     2821 cattaaactt cttttttcag aaagcggtga tttaatttcg ctatatgata aaagatatgg
     2881 taaagaatac attactgaaa atatgcacag tgagatccgt gcataccacg aagatgcagg
     2941 ttttttcgcg gcctgggatt ttgcttcaaa ttatcgtgat ggggaatcat atgttttact
     3001 cgctgaaaaa atgacaactg tgatcagcgg gcctaaaact acgatgacgc tgatttatca
     3061 ctacaattcc tcttatcttc gtttagcctt taccctgaca caggatagtc cacgcgttga
     3121 tgtccaaacg tttattgatt ggcacgaacc taatgtttct ctcaaagtaa aattcccggt
     3181 gtcagtacaa acatctcttg cacaatgtca aattcagttc ggtgtaattg acagaccaac
     3241 gcatagcgat gattcgttcg cttttgccaa agatgaaata ccagcacatc actgggtcga
     3301 cctttctgat cgagaaactg gcatagcttt aatagcaaaa aacaaatatg gctatcgtgt
     3361 caaagaccag acgcttgaat taactttgct gcgttcgcag cataaacctg gggaagttgt
     3421 taagacagac aattatgata atttcctgat taataatttt gcagatattg gtaaacaaaa
     3481 attcatattc tccttatttc ctcatcatgg agattaccgt gtgggtggtg ttattaatca
     3541 agcctatata atgaatcatc ccttacaggt cgtccctgtc aaaccgcacc cgggtgaatt
     3601 acccccttca ctatcatttt ttgctatcga tactaacagc gttatcatcg aaacaataaa
     3661 attagctgaa gacggcgacg gtataatcgt aagattatat gagagtcatg gattagaaat
     3721 ttcagccatg ctttcgtgag tatgtaatta tcactacgtt caatatgtcg atcttcaaga
     3781 aaataagtta gatgacagtt tttattgcaa tcaatattgc aatcttgagt tcaaaccttt
     3841 tgaaattatc acgctacgat taactcaata gcgtactaac ctaaaaactc aaaatagtgg
     3901 gaacactcat tgagattttt tatcgagtac agcctctcaa taatttgaga ggcttttttt
     3961 atttgatagg ataaagatct ttacgcttat acggttggat ttcgcccggt ttgcgagttt
     4021 tcagcaattt taatatccag gtgtattgtt ctggtcgcgg accaacaaaa atctcgactt
     4081 cttcattcat ccgccgcgca atcgtatgat catccgcctc taacagatca tccatcggtg
     4141 ggcgcacctg aatcgtcaga cggtgcgtct tgccatcata aatcggaaac agcggaacaa
     4201 cgcgcgcacg gcacactttc atcaaacgac caatcgcggg caacgtcgct ttataggtgg
     4261 caaagaaatc cacaaattcg ctgtgctctg ggccatgatc ctgatcgggt aaataatatc
     4321 cccagtaccc ctgacgtacc gactggatga atggtttaat accgtcattt ctcgcatgca
     4381 gacgaccgcc aaagcgacga cgcaccgtgt tccagacata atcaaaaacc gggttgccct
     4441 gattatggaa catcgctgcc attttctgcc cttgcgaggc catcagcatg gcaggaatat
     4501 cgacggccca accgtgcggc accagaaaga taactttctc gttattacgc ctcatctctt
     4561 cgatgatctc cagcccttgc cagtcaacgc gcggctgaat tttctccggt ccgcgtattg
     4621 ccaactcagc catcattgcc atcgcttgcg gcgcggtggc aaacatctca tcaacaatcg
     4681 cttcgcgttc agcttcacta cgttctggaa agcagagcga cagattgatt aacgcacgac
     4741 ggcgtgagct ttttcccagt cgtccggcaa aacgtcccag ccgtgccaga atgggatcac
     4801 ggaactttgg cggcgttaaa gcgatacccg ccatcgctgc tacacccagc catgctcccc
     4861 agtagcgcgg gtggcgaaag gatttatcaa actcaggaat gtattcgcta ttattttttt
     4921 tcgtttccat gcttttccag tttcggataa ggcaaaaatc aatctggtga tagtgtagcg
     4981 gcgcaacttg ccccgcacca aataaaaaaa gccgatactg actgcgtacc ggctgcgaat
     5041 ggatgttaat taatcaaacc gtagctgcgg cacaatctct ttggcctgcg ccaggaattc
     5101 gcgacgatcg gagccggtca gcccttcggt acgcggcagt tttgccgtca gcgggtttac
     5161 ggcctgctgg tttatccata cttcatagtg cagatgcggc ccggttgaac gtccggtatt
     5221 accggaaagc gcgatacggt cgccacgttt caccttctgt cccggtttca ccagaatctt
     5281 gcgcaagtgc atataacgcg tggtgtagct gcgaccatga cgaatagcca cataataacc
     5341 tgctgcgcca ctgcgtttgg caaccaccac ttcaccgtca cccactgaaa gcactggcgt
     5401 accctgcggc atggcgaaat caacacctct gtgtgctgca acgcgaccgg tcaccggatt
     5461 agtacgacgc gggttaaagt tagacgagat acggaactgt ttcgccgtcg ggaatcgcaa
     5521 gaatcctttc gccagaccag taccgttacg atcgtagaat ttgccatctt cagcgcggat
     5581 agcgtaataa tctttacctt ctgaacgcaa acgtacgccc agcagctggc tttgctcacg
     5641 tttaccatca agcatttctc gagacattaa caccgcaaat tcatcgcctt ttttcagttt
     5701 gcggaaatcc atttgccact gcatggcttt aatcactgcg ctcacttcgg cgctggttaa
     5761 accggcgttt ctggcgctgg caacaaagct ccccccgacg gtacctttca gcagattgtt
     5821 aacccactct ccttgctgca tttcgctggt cattttaaaa ccgttagcgg cagtacggtc
     5881 ataggttcgg gtttcacgac gagacacttc ccaggtgagg cgctgcagtt cgccgtccgc
     5941 ggttaatgtc caggagagtt gttgaccgat tttcaggtta cgcaattctt tgtcggccgc
     6001 agccagttgg gtgatatcac ccatatcaat accatactga ttgagaatgc tgcttagcgt
     6061 atcgccagtg gaaacaacat attcatgcac gcccgcttca ccggcgattt tgtcatccag
     6121 ttcgtcctgg ggaatggctt catcttcttg tgcagcttga tcaatcggct cactggcttc
     6181 aggtaagagc gaacgaattt cgttctgttc cagctcaatg gttttgacaa ttggcgtggc
     6241 atcgcggtga taaacatagg gccgccagac agcgacggcc agagtaagaa cggtgagcga
     6301 ccccaacata acgcggtgtg gtcgcggtaa attattaaac gccagggcga cagagcgggc
     6361 tatctgttgc acgtaatcac ttcctcatta atctcctttc aggcagctcg catactggtt
     6421 ggctaattga ctcaggaatt ctgaatagct tgttttaccc agtttgatat tcgtccccag
     6481 gggatccaac gttcccatac gaacggatgt ccctcgtgcg acgctctcaa cgaccgctgg
     6541 cctgaactgt ggctcagcaa aaacgcaggt tgctttttgc tcaaccaact gtgttcttat
     6601 ttcatgtaaa cgctgcgcgc caggttgaat ctcagggtta acggtaaaat gaccaagcgg
     6661 tgtcagtccg aactgttttt cgaaatagcc gtaagcatcg tgaaaaacga aataaccttt
     6721 ccccttgagc ggcgcgagct cgttaccaac ctgcgtttcg gttgaggcta attgtgcctc
     6781 aaaatccttc aggttggcgt caagtttggc tcgactttgc ggcataagtt ccactaattt
     6841 tccatggatt gcaaccgctg tagcccgcgc tatctctggg gaaagccaaa gatgcatgtt
     6901 gaaatcgccg tgatggtgat cttcgtcact tttttccgcg tggtcgtgat catcatcatc
     6961 gccgtgaata cttttcatca gtagcggttt cacatcttca agctgcgcaa tcgttacctg
     7021 cttcgctcct ggtaatttgc ttaccggttt ttgcataaac gcttccatct ccgggccaac
     7081 ccaaacgact aagtccgcgt tctgtaagcg ttttacatcc gatgggcgca gtgaataatc
     7141 atgttctgaa gcgccgtcag gaagtaaaac ctctgtttct gttaccccat cagcaatggc
     7201 agaagcgatg aacccaacgg gtttaagcga agcgacaacg gcggcatctg cggcctgtgt
     7261 tgcacctccc cagagagcgg cggataatgc tgcgaaaaga agcgtttttt tatgtaacat
     7321 aatgcgacca atcatcgtaa tgaatatgag aagtgtgata ttataacatt tcatgactac
     7381 tgcaagacta aaattaacat gacaagtctg gtttccttgg aaaatgtctc ggtttctttt
     7441 ggccaacgcc gcgtcctctc tgatgtgtcg ctggaactta aacctggaaa aattttgact
     7501 ttacttgggc caaatggcgc aggtaagtcg acactggtac gggtagtgct cgggctggta
     7561 acacccgatg aaggggttat caagcgcaac ggaaaactgc gcatcggcta tgtaccgcag
     7621 aagctgtatc tcgacaccac gttgccactg accgtaaacc gttttttacg cttacgccct
     7681 ggtacacata aagaagatat tttgcctgca ctgaaacgtg tccaggccgg gcacctgatt
     7741 aacgcaccga tgcaaaagct ctctggtggc gaaacgcagc gtgtactgtt agcgcgagca
     7801 ttgttaaatc gcccgcaatt attagtgctg gatgaaccca ctcaaggcgt ggatgtaaat
     7861 ggccaggtgg cgttatatga ccttattgac caactgcgtc gtgaactgga ttgtggcgtt
     7921 ttaatggttt ctcacgatct gcatctggtg atggcaaaaa ccgatgaagt gctgtgcctg
     7981 aatcaccaca tttgttgttc cggcacaccg gaagttgttt ccctgcatcc ggagtttatt
     8041 tcaatgtttg gtcctcgtgg tgctgaacaa ctgggtatct atcgccatca tcataaccat
     8101 cgtcacgatt tacagggacg aattgttttg cgtcggggaa atgatcgctc atgattgaat
     8161 tattatttcc cggttggtta gccgggatca tgctcgcctg tgccgcgggt ccgctgggtt
     8221 cgtttgtagt ctggcgtcgt atgtcttatt tcggtgatac gctggctcat gcctcattac
     8281 ttggtgtcgc gtttggtttg ttgctggacg tgaatccatt ctatgcggtg attgccgtta
     8341 cgctgctgct ggcgggcggt ctggtatggc tggagaagcg tccacagctg gcgatcgaca
     8401 cgttattagg gattatggcg cacagtgccc tgtcgctggg cctggtggtc gttagtctga
     8461 tgtctaatat tcgtgttgat ttgatggctt acctgttcgg tgatttgctg gcagtgacgc
     8521 cagaagatct catctctatt gcgattggcg tggtcatcgt ggtggctatt ttgttctggc
     8581 aatggcgcaa tttgctgtcg atgacgatta gcccggatct ggcgtttgtt gatggtgtga
     8641 aattacagcg cgtgaaattg ttgttgatgc tggtgacggc attgacgatt ggtgtagcga
     8701 tgaaatttgt cggtgcgttg attattactt cgttgctgat tattcctgca gctactgcgc
     8761 gtcgctttgc ccgtacgccg gaacagatgg ctggtgtcgc tgttttggtg gggatggtgg
     8821 cagtaactgg cggtttaacc ttttccgcgt tttacgatac gccggcgggt ccgtcggtag
     8881 tgctatgtgc tgcactgtta tttattatca gtatgatgaa aaagcaggcc agctaatctg
     8941 tcgctgaaca catttgtcgg atgcggcgcg agcgccttat ccgacctacg gttcgctatc
     9001 tctggtaggc cggataagac gcgaatagcg tcgcatcagg cacactgcca atgtcggatg
     9061 cggtgcgagc gaccaatccg acttacggca tttctggcgg cgttatgcca aagtgattcc
     9121 acgcccgcgt cgtcgccata cgcccacgcg gtgtacgctg caaaaagcct tgctgaatca
     9181 gataaggctc cagcacatcc tcaatggttt cacgttcttc gccaatggct gccgccaggt
     9241 tatccagacc taccggccca ccaaagaact tatcgattac cgccagcaac aatttgcggt
     9301 ccatataatc gaaaccttca gcatcgacat tcaacatatc cagcgcctga gcagcgatat
     9361 ctgccgagat ggtgccatcg tgcttcactt cggcgaaatc acgcactcga cgcagcagac
     9421 ggttggcaat acgtggcgta ccgcgagcgc gacgagcaac ttccagcgcg ccgtcatcac
     9481 tcatctcaag ccccataaag cgtgcgctgc gactgacgat atattgcaga tccggcacct
     9541 gataaaactc cagacgttgc acaataccaa aacggtcgcg caacggtgat gtcagcgaac
     9601 ctgcgcgcgt ggttgcacca atcagggtaa acggcggcaa atcaatttta atggagcgtg
     9661 ccgccggacc ttcaccaatc atgatatcca gttggtagtc ttccattgcc gggtacagca
     9721 cttcttcaac aactggcgat agacggtgga tctcatcaat aaacagcacg tcatgcggtt
     9781 caaggttagt gagcatcgct gccagatcgc ccgccttttc cagcaccgga ccagaagtcg
     9841 tgcgtaaatt aacgcccatt tcattggcga caatgttggc aagcgtagtt ttacccaacc
     9901 ccggaggacc aaaaatcaac agatgatcga gggcatcgcc gcgcagtttc gctgctttga
     9961 tgaaaatctc catctgtgaa cgaacctgcg gctgaccaac atactcttcc agtaatttag
    10021 ggcgaatggc gcgatctgcc ccatcttccg gcaaagtggt accggcagaa atcagacggt
    10081 ctgcttcaat catcctttac ctcataacgc ggcgcgtagg gcttcgcgaa ttaatgtttc
    10141 actgctggcg tcagtgcgag cgattttgct caccatgcgg cttgcttctt gtggtttata
    10201 gcccagcgcc accagcgcgg caaccgcttc ctgttcagca tcgtcggttg ccgggctggc
    10261 aggcgacgtg agcaccagat cggcggctgg cgtaaagaga tcgccgtgca aacctttaaa
    10321 tcggtctttc atttcgacaa ttaaacgctc ggcggttttt ttaccaatgc ccggcagttt
    10381 caccagtgct cccacttctt cacgttcaac ggcattaacg aactgctgcg ctgacattcc
    10441 ggagaggatc gccagcgcca acttcgggcc gacgccgttg gttttgatta actctttaaa
    10501 caacgtgcgc tcttgtttat tgttaaaacc gtacagcagt tgcgcgtctt cacgcaccac
    10561 aaagtgggtg aaaacgatcg cttcctgacc cgcttcaggg agttcataaa aacaggtcat
    10621 cggcatatgc acttcatagc ctacgccgcc cacttcaatt aacaccagcg ggggttgttt
    10681 ttcaatgatg atgcctctga gtctgcctat cacatgacgc tcctgcgtaa tgaatcaaag
    10741 ataatgctgt atgataaaaa aatgctggat agatatccag cgaaggatga agaaaacttg
    10801 cgaggtgtct cgatgaactg aaaaatggca cagtataatt tattctacag gttatattgg
    10861 aagcaaatat tttaatatta catattcagt gaataaatgc gtaataaaaa tatacattgc
    10921 gcctcctgaa aaaaaataaa ttttttatgc tattacgtat attcgtatct atttcaatgg
    10981 aatgacaacg tgaatattaa ttaccctgct gaatatgaaa ttggagatat cgtctttaca
    11041 tgtataggtg ctgccttatt tggtcaaata tcagctgcat caaattgctg gagtaatcac
    11101 gtcgggatca ttatcggtca taacggtgaa gacttcctgg ttgcagaaag ccgggttccc
    11161 ctttcaacca tcaccacgct atctcgtttt attaaacgtt cgtctaatca acgctatgct
    11221 ataaagcgat tagacgctgg gctgacagaa caacaaaaac aacgaattgt tgaacaggtt
    11281 ccctcccggc tacgcaaact ttaccacacc ggttttaaat acgaatcttc gcgccagttc
    11341 tgttcaaaat ttgtttttga tatttataaa gaagcgctat gcattccggt gggtgaaata
    11401 gaaacgtttg gagaactgtt aaatagcaat ccgaatgcaa aactcacttt ctggaaattc
    11461 tggttcttag gttctattcc gtgggagcgt aaaaccgtca cgccagccag tttgtggcat
    11521 catccgggtc tggtgttgat tcacgcggag ggagttgaaa cgcctcagcc tgaactgacc
    11581 gaggcggtat aacttaacgc agtcgccctc tcgccaggtt cagccgcgat tcgctcatct
    11641 gcattgcatt ctggctgacg tgacaatggg taatcgcgat agctaacgca tcggcggcat
    11701 ccgcctgtgg attagcgggc agtttcagca aggtgcggac catatgctgc acctggcttt
    11761 tttcggcact accaatacct accaccgttt gctttacctg acgtgccgcg tattcaaata
    11821 ccggcaattc ctgattcacc gccgccacaa tcgccacgcc gcgcgcctgc cccagtttca
    11881 gagccgagtc ggcatttttg gccataaaga cttgttcaat ggcgaaataa tctggctgga
    11941 actgggtgat gatttccgtc acgcccgcat agatgagctt cagacgagac ggtaaatcat
    12001 ccactttggt gcgtatgcat ccgctaccca ggtaggacag ttgcctgcct acctggcgga
    12061 tgacaccata gccgatcacg cgcgaacccg ggtcaatgcc gagaataata gccatcacgc
    12121 gtctccgttt tgctgtttag caggccccat cagagagttg ctgcaacctc atcagagatt
    12181 tcaccgttat ggtagacttc ctgcacgtcg tcgcaatctt ccagcatatc gatcagacgc
    12241 atcagtttcg gtgcggtttc tgcatccata tcagctttgg tggacgggat catggaaact
    12301 tccgcgctgt ctgctttcag acctgccgct tccagagcgt cgcgcacttt acccatttct
    12361 tcccatgcag tgtagacgtc aatcgcgccg tcatcatagg tcacaacgtc ttcagcacct
    12421 gcttccagtg ctgcttccat aatggtgtct tcatcgcctt tctcgaagga gatcacgcct
    12481 tttttgctga acagataggc aacggaacca tcagtaccga ggttaccgcc acatttgcta
    12541 aatgcatgac gcacttcagc aacggtacgg ttgcggttgt cagacagaca ttcaatcatg
    12601 attgccgtgc cgccaggacc gtaaccttcg tagatgatgg tttccatgtt tgcatcatca
    12661 tcaccgccca caccacgtgc aattgcgcgg ttcagtgtgt cacgggtcat gttgttagac
    12721 agtgctttat ccaccgccgc acgcagacgc gggttagcgt ccggatcgcc accgcccagc
    12781 ttagccgcgg ttaccagctc acgaatgatt ttagtgaaga ttttaccgcg cttagcatcc
    12841 tgcgcagctt tacgatgtct ggtgttggcc catttactat gacctgccat aaaaatatct
    12901 ccagatagcc ctgcctgttc aggcagcgtt aattacaaac tgttcaatcg cctgccggtt
    12961 gctccaggac ttagtgagcg ccgcggcagc aggcgcatca agccacttgt aagccagatg
    13021 ttcagtgaag actacctggc gttcgtgcgg gagcgcaaga cagaaccatg attccgtatt
    13081 acgcgtcacg cccggcgcat agcgatgacg taaatgtgaa aaaatttcaa actctaccgt
    13141 gcgctgacag tcaattaagg tcagttgttc agcgacaaca tcaatggtga cctcttcctt
    13201 tacttcgcgc atggcagctt gcggcgcggt ttcaccctct tccacgctgc cggttaccga
    13261 ctgccagaaa tcgggatcgt cacgccgctg caacatcagc acccgtttcg tatcttgtgc
    13321 atagatgacc actaagatcg aaacgggacg cttatacact ttatccttca cgctgcctct
    13381 tcgttgactg ccttcgctca tcccattcac atagttatct atgctcatgg gagttcactc
    13441 agttgccgcc ttgatgcaac gcgaatgatt tcgtgtattt gagtcatatc agttattctc
    13501 agccttcttc acaacctgaa tgctcagctc agccagtaca gtcgggttag caaagctcgg
    13561 tgcttcagtc atcagacacg ctgccgccgt ggttttcggg aaggcgataa cgtcacggat
    13621 attgtcggtg ccagtcagca gcatggtcag acggtcaaga ccgaatgcca gacctgcgtg
    13681 cggcggagta ccgtatttca gggcgtcgag caggaagccg aatttctcgc gctgttcctc
    13741 ttcgttgata cccagaatac caaacaccgt ctgctgcata tcaccattat ggatacgtac
    13801 tgaaccaccg cccacttcgt aaccattgat gaccatatcg taagcgttcg ccaccgcatt
    13861 ttccggtgca gctttcagtt ctgcagcagt catgtctttc ggtgaggtga acggatggtg
    13921 cattgccgtc aggccgcctt caccgtcgtc ttcaaacatc gggaagtcga taacccacag
    13981 cggtgcccat ttgctttcgt cggtcagacc aaggtcttta cccactttca ggcgcagtgc
    14041 gcccatcgcg tcggcaacaa ttttcttgtt gtcggcaccg aagaaaatca tatcgccatc
    14101 ttgcgcggca gtacgatcca ggatggcttc gatgatttct gcattaagga acttcgctac
    14161 cgggctgttg ataccttcca gacctttcgc gcgttcgtta actttgatgt aagccagacc
    14221 tttcgcgccg tagattttaa cgaagttacc gtattcgtcg atctgcttac gggtcagcga
    14281 tgcgccaccc ggaacacgta gagcggcaac acggcctttc ggatcgttcg ccggccccgc
    14341 aaatactgca aactcaacag atttcagcag atcggcaacg tcggtcagtt ccatcgggtt
    14401 acgcagatcc ggtttatcag aaccataacg gcgttctgct tccgcaaagg tcattaccgg
    14461 gaaatcgccc agatccacgc ccttcacttc cagccacaaa tgacgcacca gcgcttccat
    14521 cacttcacgc acttgcggcg cggtcatgaa agaagtttcc acatcgatct gagtaaattc
    14581 aggctgacga tcagcgcgca ggtcttcgtc acggaagcat ttaacgatct gatagtaacg
    14641 gtcaaaaccg gacatcatca gcagctgttt gaacaactgt ggggattgcg gcagtgcgta
    14701 gaatttacct ttgtgcacac gagaaggcac caagtagtca cgcgcgcctt ccggcgtggc
    14761 tttggtcagc atcggagttt cgatgtcgag gaagccgtgg tcatccataa aacggcgcac
    14821 caggctggtg attttagcgc gggttttcag gcgctgcgcc atttccgggc gacgcaggtc
    14881 gaggtagcgg tatttcagac gcgcttcttc ggtgttgacg tggttagagt caagcggcag
    14941 aacatctgcg cggttgatga tagtcagcga ggacgccagc acttcaattt cgccggtcgc
    15001 catatcgcgg ttaatatttt tttcgtcacg cgcacgtacg gtgcccgtaa cctgaatgca
    15061 gaactcatta cgcagttcag aggccagctt taacgcgtcc gcacgatccg gatcgaaaaa
    15121 tacctgcacg ataccttcgc ggtcgcgcat atcgatgaag atcaggctac caagatcacg
    15181 acgacggttg acccaaccac acagagtcac ctgctgcccc acgtgggaca aacggagctg
    15241 tccacaatat tctgtacgca tgagatatcc cttaacttag ctgccggcgg atgccccctg
    15301 ctgcgcaggt gaccaagtcg cagcgttagc tgtatgtcac aactgaatga aaaaaggcgg
    15361 ctattatact ggaaattctg ccgcaccgta agagcctggc ccgcgctgta acgcctcgtt
    15421 accactttat atcgggcctg aaatcagact ctacgccagt ttgctataaa ggtgttgccc
    15481 gaactcataa aaattaacaa aatttgtcgt tccgccatcg gctaatcgca ttaaggtgtg
    15541 aggcacgatt ttgttttttc aggagtcagc atgcttgaac ttaatgctaa aaccactgcg
    15601 ttggtggtga tcgatttaca agaaggcatc ttaccttttg ccggtggtcc acatactgcc
    15661 gatgaggtag ttaatcgcgc cgggaagctg gcggcgaaat ttcgcgccag cggtcagccc
    15721 gtgtttctgg tgcgagttgg ctggtctgcc gattacgccg aagcattaaa acagccggtt
    15781 gatgccccct cccccgctaa agtgttgccc gaaaactggt ggcaacatcc agctgcatta
    15841 ggtgcaactg acagtgatat cgaaatcatc aaacgtcagt ggggtgcgtt ttacggtacg
    15901 gatctggagt tgcaactgcg ccgccgaggt atcgatacaa tagtgttatg tgggatctcg
    15961 accaatatcg gtgttgaatc caccgcccgc aatgcctggg aacttggctt taatctggtg
    16021 attgccgaag acgcctgtag tgccgccagc gccgagcaac acaataacag cattaatcat
    16081 atctacccgc gcatcgcccg tgtgcgtagc gtggaagaga tccttcacgc gttatgattt
    16141 acatcggtct accgcaatgg tcgcatccta aatgggtgcg gttggggatc accagccttg
    16201 aagagtatgc ccgccacttt aactgcgtga tacgggattt ttaaaaatca ctaaagaacg
    16261 cccaagagca tgtgttttct ttagaataat caaagcgttg gattgccatg ccatatgaaa
    16321 atcggcaaag ttcatatgta acaaagtgtc ccaatcatgc cccaaaatgc cccacaacaa
    16381 atatttttgc cccatgcatg ccccaaaaaa tcactttccc tcactcttga cactgtttat
    16441 ccatacagtt aaaaataata ctgtataaaa acacagtgta gagggatttt tatgcgtatt
    16501 gaaatctgca tagccaaaga gaaaatgact aaaatgccaa ccggtgctgt ggatgcgtta
    16561 aaggaagaat taacccgacg catcagtaaa cgttatgacg atgtagaggt gatcgtaaaa
    16621 gccaccagca acgatggcct ttctgttacg cgcaccgccg ataaagattc agctaaaact
    16681 tttgttcagg aaactctgaa agatacctgg gagtctgctg acgagtggtt tgttcactaa
    16741 ttagcacgta aaatcagtaa cggctggaaa tcattcaata ctcgcactat cggaagttca
    16801 ccagccagcc gcagcacgtt cttgcatacg acgtgtctgc ggtctcaact ccgcccgccc
    16861 ccgccaatca tgattgggca gtaccagtac agcactagca aagacaggaa attagcagtc
    16921 tcagcaaaac accgaccaga cggtgagaag acaaaaagat acgcaaagat gccgcggttc
    16981 cagaagaata aaaaaccgct aatggacagc ctccttaaca cagtttttta tatacatgat
    17041 tcactgatac cagtggcatt aatagctatt atatagcttc agctcctttt ttcatacctt
    17101 aaggaaaaat tgccccctcc cccaaaaagt atcgtttttt tacattaaac cgatccagtt
    17161 taggatagtg taaagcagaa gttcttcaat atataacata tggagttaat atgcttacac
    17221 aaactatata tccgcctcct ccagaagttc ctgtctactg ctgaagggaa ccaataacat
    17281 tgcaaagcag ataaaaaaga atccaacacc tctcaacata aagaatacgc cctgatattt
    17341 atgctcccga caaataacaa tcacagatta atttcaaatt cgttctccac ttattcaatc
    17401 gacactagcc gtgcatatga aaattatcta acccattgga ctgaatggaa aaataaccgc
    17461 atacaagaag aacaacgaga catcgctttt cagcgactag tatcatgtct acaaaaccaa
    17521 gagacgaacc tggacttgtc tgaattaggc ctgacaacat tacctgaaat ccccccggga
    17581 attaaatcaa ttaatataag taaaaataat ttaagcttaa tctccccatt gcctgcgtcc
    17641 cttacacagc ttaatgtcag ctataacaga cttattgaac tgcctgcttt gcctcaagga
    17701 cttaaattat tgaatgcgtc ccacaatcaa ctaatcacac tacccacact ccccatatct
    17761 ttgaaggagc ttcatgtctc aaataatcaa ttatgttctc ttcctgtttt accagaacta
    17821 ctggaaacat tagatgtatc atgtaatggg ctggcagttt taccaccttt accattttct
    17881 ttacaagaga ttagcgcaat agggaatctt cttagtgaac tcccccctct acctcacaac
    17941 attcactcca tatgggcaat cgacaatatg ttaaccgata ttccatacct gccggaaaat
    18001 ttaaggaacg gttattttga cataaatcag ataagtcata tcccggaaag cattcttaat
    18061 ctgaggaatg aatgttcaat agatattagt gataacccat tgtcatccca tgctctgcaa
    18121 tccctgcaaa gattaacctc ttcgccggac taccacggcc cgc
//
LOCUS       DAKISA010000090        17971 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.090, whole genome shotgun sequence.
ACCESSION   DAKISA010000090 DAKISA010000000
VERSION     DAKISA010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17971
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.090"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(228..1202)
                     /gene="cysB"
                     /locus_tag="OVW45_003140"
     CDS             complement(228..1202)
                     /gene="cysB"
                     /locus_tag="OVW45_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCV5527526.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(1412..4009)
                     /gene="topA"
                     /locus_tag="OVW45_003141"
     CDS             complement(1412..4009)
                     /gene="topA"
                     /locus_tag="OVW45_003141"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCV5527527.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTIGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKIGEIVTDRLEENFRELMNYDFTAQMENSLDLVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            4389..4640
                     /locus_tag="OVW45_003142"
     CDS             4389..4640
                     /locus_tag="OVW45_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCV5527528.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FIGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(4676..5725)
                     /gene="sohB"
                     /locus_tag="OVW45_003143"
     CDS             complement(4676..5725)
                     /gene="sohB"
                     /locus_tag="OVW45_003143"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCV5527529.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQHKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            5945..6703
                     /locus_tag="OVW45_003144"
     CDS             5945..6703
                     /locus_tag="OVW45_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCV5527530.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            6700..7290
                     /gene="cobO"
                     /locus_tag="OVW45_003145"
     CDS             6700..7290
                     /gene="cobO"
                     /locus_tag="OVW45_003145"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCV5527531.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(7330..8202)
                     /gene="rluB"
                     /locus_tag="OVW45_003146"
     CDS             complement(7330..8202)
                     /gene="rluB"
                     /locus_tag="OVW45_003146"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCV5527532.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(8415..9529)
                     /locus_tag="OVW45_003147"
                     /pseudo
     CDS             complement(8415..9529)
                     /locus_tag="OVW45_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
     gene            complement(9557..10177)
                     /locus_tag="OVW45_003148"
     CDS             complement(9557..10177)
                     /locus_tag="OVW45_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCV5527533.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(10174..11055)
                     /gene="yciV"
                     /locus_tag="OVW45_003149"
     CDS             complement(10174..11055)
                     /gene="yciV"
                     /locus_tag="OVW45_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCV5527534.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEVLVHRAVEMRVGTLAITD
                     HDNTAAIVPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGARRLAQGGAVTRGHFARFLVGCGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            11193..11237
                     /gene="trpL"
                     /locus_tag="OVW45_003150"
     CDS             11193..11237
                     /gene="trpL"
                     /locus_tag="OVW45_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCV5527535.1"
                     /translation="MKAIFVLKAWWRTS"
     misc_feature    11214..11307
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            11329..12891
                     /gene="trpE"
                     /locus_tag="OVW45_003151"
     CDS             11329..12891
                     /gene="trpE"
                     /locus_tag="OVW45_003151"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCV5527536.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSSYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            12891..14486
                     /gene="trpD"
                     /locus_tag="OVW45_003152"
     CDS             12891..14486
                     /gene="trpD"
                     /locus_tag="OVW45_003152"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCV5527537.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPILMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHH
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            14487..15848
                     /gene="trpCF"
                     /locus_tag="OVW45_003153"
     CDS             14487..15848
                     /gene="trpCF"
                     /locus_tag="OVW45_003153"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCV5527538.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIASIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLILVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            15860..17053
                     /gene="trpB"
                     /locus_tag="OVW45_003154"
     CDS             15860..17053
                     /gene="trpB"
                     /locus_tag="OVW45_003154"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCV5527539.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            17053..17859
                     /gene="trpA"
                     /locus_tag="OVW45_003155"
     CDS             17053..17859
                     /gene="trpA"
                     /locus_tag="OVW45_003155"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCV5527540.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISTPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKVATRS"
BASE COUNT         4319 a         4756 c         4490 g         4406 t
ORIGIN      
        1 ccagcgaatt tacgaatcgt ctccagtgaa taattcaatc ttttgacctg aaatagatat
       61 ttgaaaactg gagtttgcga ggcgggtaat tagacactac tttacaggta atttacccta
      121 taaatatgag ggataaaaat attgtgcacg gtatagaaat aagtaacaaa agagaatata
      181 ttccggcacc ttcgctacat aaaaggtgcc gaaaataacg caagaaatta tttttccggc
      241 agttttatat ctttaaacat gacctcaatt tcttcattag agcgcaatgc gacagccgca
      301 tcaacgacat cacgcgttaa atgcggtgca aaacgctgaa tgaaatcata catataactg
      361 cgcaagaaag tactacggcg aaaaccaatt ttggttgtac tgtggctgaa gatatcgtga
      421 gcatcaacac gcacaaggtc gggatcggcg accggatcca ccgccatgct ggcaatgacc
      481 cctaccccca gccctaaccg gacgtaagtt ttaatgacgt cagcatccgt tgccgtgaag
      541 acgatacgcg gcgttaaccc tgcgcggtta aaggcagtat ccagttctga acgtccggta
      601 aagccgaagg tatatgtcac caacggatat tgcgccagtt cttcaatggt aatggctttt
      661 ttgcctgcca gcgggtgatc cggagtgact acaatagccc gattccagtg gtagcacggt
      721 aacatcacta aatcttcata cagatgcagc gcttctgtgg cgatagcgaa atcagcattg
      781 cctttagaga cggcatcagc aatttgtgtc ggcgagccct ggtgcatatg caaagaaacg
      841 cggggataac gctcaataaa gcctttgata acgtttggta acgcgtagcg tgcctgggta
      901 tgcgtggtgg cgatatacag tgagccttta tccggccagg tgtgctctcc ggcaaccgat
      961 tttatggcat cgactttcga caggacttcg cgagcgatac gaattatttc ttgccctgct
     1021 ggcgttacct gcgtcaggtg cttgccgctt cgggaaaaaa tttgaatgcc tagctcgtct
     1081 tccagcattc tgacttgttt actgatcccg ggttgtgatg tgtaaagtcc ttccgctgtt
     1141 gatgagacat tcaggttatg attgaccacc tcaacaatat agcgaagttg ttgtaatttc
     1201 atgttaaacc atccacttag acgccatcag aacatcgttt tttcgtcaca cctggcgggt
     1261 gactaatacg atttaataat aaaaaaggtg ctaactataa ccactatatc atttatagcc
     1321 ttagtgtata ggtgtgaaga aaaaataata tgcaataaaa aagggccgct ttcgcgaccc
     1381 tttgtttata aaaacctgac agaattaaag gttatttttt tccttcaacc catttgccat
     1441 caacataaaa tgctgaccag ccagtcgcct ttccgtcttt ttccgaagag acatattgct
     1501 gtttggtttt acggctaaag cgaaccatgg tcttattacc ttccggatcc tgctgtggcg
     1561 catcggccag ataacgcagt ttttccggca gacggtcgcg gaagcgataa agctcttcca
     1621 ccagtggcgc acgcgtttca cgcgatttcg ggaaagtgtt ggcagccagg aaaacacccg
     1681 cagcgccgtc acgcagcacg aaataagcat ctgatttttc gcacggcagc tcaggtaatg
     1741 gcaccggatc ttctttcggt ggtgccactt cgccgttacg taaaatctta cgtgtgtttt
     1801 tacactcttc gttggtgcag gccatgtatt taccgaaacg ccccattttc aggtgcattt
     1861 cagagccaca tttttcacac tcaacgatcg ggccgtcata acctttaatg cggaattcgc
     1921 cctcttcgat ctcgtaaccg tcgcaggttg ggttattacc acagacatgc aacttacgtt
     1981 tcggatcgat gagatagctg tccatcgccg tgccgcattt cgggcaacga cgttttgcgc
     2041 gcagcgcgtt ggtttcagcg tcttcgcctt ccagtacgtt cagcacttcg ttttccggca
     2101 ccaggttaat ggtggttttg caacgctctt tcggcggcag cgcataacca gaacagccaa
     2161 ggaatacccc ggtgctcgct gtgcgaatcc ccattttgcg accacaagtc gggcagtcaa
     2221 tgctggtcag aaccatctgg ttcgggcgca taccaccctc ttccggatct ttttcagctt
     2281 tatctaactg ctgggtgaaa tccgagaaga agtgatccag tacagctttc cactctgctt
     2341 cgtgatttgc caccaggtcg aggctgtttt ccatctgtgc ggtgaagtcg tagttcatta
     2401 actcgcggaa attttcttca aggcgatcgg tgacgatttc gccaattttt tccgcataga
     2461 aacgacgatt ttctactcgc acgtagccac gatcctgaat ggtcgaaatg atcgacgcat
     2521 aggtagacgg acgaccgata ccgcgttttt ccagctcttt aaccagcgat gcttcactga
     2581 aacgggctgg cggcttggta aagtgctggg ctggtgtaag ttcaacgagc gtcagagcat
     2641 cgcctttatt aactgctggt aagatgcgat cttcatcgcc tttacgcaac gcaggcatca
     2701 cttttgtcca gccatcaaaa cgcaaaatac gaccgcgtgc tttcaggcgg aaatcgcccg
     2761 caccaatggt cagcgtcgtg gagtcatatt tcgctggggt catctggcag gctacgaact
     2821 gacgccagat taactggtac agtttctgcg catctgcttc catatccttc agcgattccg
     2881 ccatcacatt gacgtcagaa ggacgaatcg cttcgtgcgc ttcctgtgag ttttctttgc
     2941 tggcgtactg attcggactt tccggcagat atttcttacc aaaattatcg ctgatataac
     3001 cacgaaccat atttaccgcg tcctgactca ggttagtgga gtcggtacgc atgtaagtga
     3061 tatagcctgc ttcatacaaa cgctgcgcca tcatcatggt ttttttcacg ccaaatccaa
     3121 gacgggtgct ggcagcttgt tgcagcgtag aggtaataaa aggagcgcca ggtttactgg
     3181 ttgtcggttt gtcttcacgt tccagcacgc tgtagcgcgc tttttccagc agacttaccg
     3241 cagcctgagt ttgttctttg ttgaccggac ggaacggttt gtcgttctga tgagtcacct
     3301 gtaacgccaa cgcttcacca gatggcgtgg tcgtgctggc atcgacttcc cagaactctt
     3361 ccggcacgaa cgctttaatt tcacgctcac gctcgaccac caggcgaacc gccaccgact
     3421 gcacacgacc ggcagacaga ccacgagcga tctttttcca tagcagcggc gaaaccatat
     3481 accccaccac gcggtccata aagcgacgcg cctgctgggc attaacacga tcaatattca
     3541 gctcacccgg tttgttaaat gcctggcgga tcgcgttttt agtaatttcg ttaaacacca
     3601 ctcggctata gcgcgcatca tcacccccaa tcacttcccg caggtgccat gcaatggctt
     3661 ccccttcgcg gtcaaggtcg gttgcgagat agatgtggtc ggctttttca gccagttgtt
     3721 tcagttcaga gacgaccttc tctttaccag gcaacacttc atagtgcgcc tcccaattgt
     3781 gccacgggtc aacccccata cggttgacga gagcgccacg ttcatccttt ttaggctttt
     3841 tagccgtctt ggtggaggta gagtcggcac tctttttggc agctgagcca ctggtcggca
     3901 aatcgcggat gtgaccgacg ctggatttca ccacgtagtc actacccaga tacttgttga
     3961 tcgttttggc ttttgccggg gactcaacga tgacaagagc tttacccata ttcaccttac
     4021 ctaatttgat tcttccagga atgcgtcgca cgttgataaa ccttccactg gcaacgagtt
     4081 accgatatgg actcgttctc tgcggatatc aaccccttgt cgtgactctg ttcacgaaaa
     4141 ttcaggtgac tgagttaaca ggtcttggcc tacaggcgct atagcacgac ggaattttgg
     4201 tcgaatgtca agcaaatcta ttgccagatt gacgaaagcg tcacactgta cctgataaaa
     4261 tccgatacgc aactttatta gcatgcaaaa tccaaagttg ccagtaaggc gctcttgcca
     4321 ccagcgtggt atatttgtct gcaagccatg cctggcgtag actaatgtcc ttgtttaagg
     4381 agtaaataat gaacaaagag acacaaccca tcgaccgcga aacgctgttg aaagaggcca
     4441 acaaaatcat tcgtgagcat gaggatacgc tcgcagggat tgaagccacc ggcgtcacgc
     4501 agcgtaatgg cgtgctggta tttatcggcg actacttttt agatgaacaa ggattgccaa
     4561 cagccaaaag caccgcggtg tttaatatgt tcaaacacct ggcccatgtg ctttcagaaa
     4621 aatatcatct ggttgattaa agcaaaacgc gaggtcttag cctcgcgttt gtcttttaca
     4681 tcaatggctt ttgaccccgc tgccaccagc gtagcaacaa tcgatcggcg ctctctgccg
     4741 cgctgccggt gaatcggtca atgagtcgtt tacgctgcat atagcgtaca ttaaccactt
     4801 cacggccttc catcaggcta agaataactt catcactggt gttgatttca tcaaccaggc
     4861 ctttctctac cgcctgttgt ccgtaccagt gttcacccgt tgccacctgt tcaatatcca
     4921 gagacggacg catacgcttc acaaaatctt taaataactg atgcgtttcg ttcagctctt
     4981 cgcggaattt ctcccgccct tcttcggtat tttcacccag caaggtcagc gtgcgcttat
     5041 gctgcccggc ggtgtgcagt tcgatatcaa tatctttgct tttcaggaag cggttaaagt
     5101 tgggcatttg cgccaccacc ccaatggaac ccacaatagc aaacggtgcg gaaacaattt
     5161 tgtccgccac acaggccatc atgtaaccgc cgctggcagc gactttgtct accgtaacag
     5221 ttaaaggaat gtttttatca cgcagacgct gcagctgcga agccgccaac ccgtaaccat
     5281 gcaccatgcc accagggctt tccagacgga gcacaacctg atcctgcggt ttgaatgctg
     5341 cgagtaccgc cgttatctct tcacgtagcg agttcacttc atgggcgtcc atgctgcctt
     5401 taaaatccag cacccagacg cgaggtttac tgtcagttgc cacctcgccc agtttggctt
     5461 tcgcttttgc tgctttcgct tcttgcttgt gctttttctt ctgcgctttg tgccactgtt
     5521 tttgctgatg tgagtccatc agcgccgcgg ccagttcttc tttcatctcc ttatactgtt
     5581 cgctgagatt gttgacccgt aactcgccac gctggcgttt attacgttga gcaacattga
     5641 caataatggc ggcaatcgcc gcaatcgcta gcacaacggt aacgattttc gccaaaaaca
     5701 aaccatattc agacaacaat tccacgagtc cccaccttgg ttaaacaacg ctttggctca
     5761 cagtttacaa caggcatgtt aagccgtctc gcagagatgt tattgcagcg aaagtatgac
     5821 aaagtatcca ttttgcggtg ttatcgcccc aggctgattg aaatgccctt ctgtttcagg
     5881 cataaagccc caaagtcata aagtacactg gcagcgcgac agacaacgtc gttgaggagt
     5941 agccatgcat taccagccaa aacaagattt actcaatgat cgcattatcc tggtgacggg
     6001 agccagcgat gggattggtc gtgaagccgc gatgacgtat gcacgctatg gtgcgacagt
     6061 gattctgttg ggccgtaatg aagaaaaatt acgtcaggta gccagccaca taaacgaaga
     6121 aactgggcgt cagccacagt ggtttattct cgatttgctg acctgcacgt ccgaaaattg
     6181 ccaacaactg gcacagcgca ttgtcgttaa ttatccgcgt ctggatggtg ttttgcataa
     6241 tgccggattg ctcggcgatg tttgcccaat gagcgaacaa aatccgcagg tctggcagga
     6301 cgtcatgcag gtcaacgtta atgccacctt tatgctcacc caggcactgc ttcctttatt
     6361 actcaaatcg gacgccggtt cactggtctt tacttcatca agcgttggac gtcagggacg
     6421 agccaactgg ggtgcatatg cagcgtcgaa atttgccacc gaagggatga tgcaggtact
     6481 ggccgatgaa tatcagcagc gcctgcgcgt caactgcatt aacccaggcg gtacgcgcac
     6541 cgcaatgcgc gcaagcgcct tcccgaccga agatccacag aaacttaaaa cacccgctga
     6601 tatcatgccg ctctacctct ggctgatggg cgatgacagc cgccgtaaaa ccggcatgac
     6661 ctttgacgcc caaccgggcc gtaaaccagg aatttcccaa tgagtgatga acgctaccaa
     6721 cagcgtcagc agcgagtgaa agaaaaagta gatgctcgtg tggcccaggc ccaggatgaa
     6781 cgcggtatta tcatcgtctt taccggcaat ggaaaaggca aaaccaccgc ggcatttggt
     6841 acggcaacac gcgcagttgg tcacggaaaa aaagtgggcg tcgtgcagtt tattaaaggc
     6901 acctggccta atggcgaacg caatctgctg gagccacatg gcgttgagtt tcaggtgatg
     6961 gcaacgggct ttacctggga tacacaaaac cgcgagtctg ataccgccgc ctgccgcgaa
     7021 gtctggcaac atgcaaagcg gatgcttgct gattcctcac tggatatggt tttgcttgat
     7081 gaactgacgt atatggtggc gtatgactat ttgccactgg aagaagtggt gcaggcgtta
     7141 aatgaacgtc cacatcaaca gacggtgatt atcacgggtc gaggctgtca tcgggatatt
     7201 cttgaactgg cggatacggt aagtgagctg cgccccatca aacatgcgtt tgatgccggt
     7261 gttaaggcgc aaatagggat tgattattaa tctgtaaaaa tgccggatat gacgtccggc
     7321 atactttgat taaccgttat tacgcccgcc agaacgacga ccgccgctca cctgactgtg
     7381 acgtttcacc gcacgacgaa tctgattagc cttcatgcga cgacggtctt tttctacagc
     7441 gactttagag ctggtttctg gcggaagctc caccagttcg cgcagatagt tagtctgggc
     7501 gaggtccagc tccgtccagc caccgcgtgg cagacctttt ggcagtggga tatcaccgta
     7561 acgaacacgg atcaggcggc tcacctgcac accaaccgct tcccacagac gacgaacttc
     7621 acggttacgg ccttcggtca gagtcacgtt gtaccactgg ttgatccctt cgccgccgct
     7681 gaacttgatg gttttaaaag ctgccggacc atcttccagt tgcacgccac gactcaaatc
     7741 acgcagtttc gcgtcgtcaa cctgaccaaa tacacgcacg gcgtattcac gttcaacttc
     7801 acggcttgga tgcattaaac ggtttgccag ttcaccatcg gtggtgaaca gcagcagacc
     7861 acaggtgtta acgtccagac gacctacggc aatccagcgt gcgccacgca gctttggcag
     7921 acggtcaaac accgtcggac gcccttccgg atcgttacgg gtacacaact caccttccgg
     7981 tttgtaatag gccagaacgc gacaaatttg ttcagcggac tcacgtaccg aaatcagatg
     8041 accatcgata cgaattttca gcccaggggt aacttcaacg cgatcgccga gtttagcaat
     8101 tttgccatca acactcacac gaccggcttc aataatagat tcgatttcac ggcgggagcc
     8161 gtggccagcg cgggccagca ctttctgtaa tttttcgctc atagagcttc cttttggtgt
     8221 cgccttccca ggcgtctgat atgcttttgt gcaacagtga gtcagaactt acatctgact
     8281 cactgatagt aaagtctgtt tgaactcttc aggccttaag taaacgactc gacgttttaa
     8341 ctggcctggc tttgaggcgc aacatactac actatcttta tggttaaagc ttgtttcgat
     8401 taacatcagc ttaattacca gccaccaccg cctccgccac ccgcgccgcc accagcacca
     8461 ccaccggaat aacttcgccc caacaaactg gttttggccc ccagactcag gtgcatctgt
     8521 aatgaaaaca aaacgccact acgggcaatt tccggggcgc taacagccgc tgtgagtgat
     8581 ggggtgatac gtgctaccca ttctcttcca aggtttgctg agactgccca acctagcaac
     8641 gcgtaatcca gtttccgtgt ttcccctttc cgccgacgcc cactgtgtgt tgctgcctct
     8701 ttacgcgcaa gatagttaac gatagcttca ccgtgggcat aacgttgctg cccagtttgt
     8761 gtaaactttg gcagaaaaat ataccccatg ccagtgagat aaccggtcag gaaaacagcg
     8821 ctaaaataac ccgctggcat ataccagtaa aagaatacat ctccgacatt gatgtagaga
     8881 gaatagaagg ccacgcctga acaaatcagt ggtaaacaat tcttccgtcc tcaaggatgc
     8941 ggccatttcg atatgtgtcg ccctcttctc cagtaaagag atcgatttcg ctaaatggat
     9001 tacccgcgat atcagggaac ttgagtgaaa atcgggtctt atagatttca aacggccagt
     9061 ggttaccagt cacgttccag attaacagat ctgaatctcc ctcacgcagg aaagcatttt
     9121 taacctgata atgaatttga taatgatagt cgccttgagg caagaaacgt tgtttatcgc
     9181 ccacgacaat actcattaac ccgatatgcc agtcatgatg ccagggttca ggaataccat
     9241 cacgggtgac gccaacaata tgatagttca gcagtgcagc gtccccatca ggctgcatcc
     9301 agagtcgtgg taaatcacga taaaatccgt gtttaatctg ctgatttcgc gcctgaaaat
     9361 gaatattttc tttgatgtcg agcaccccat ccggttgcag agtggcttca acatcaaaga
     9421 gcgaaatccc ttcttcataa gaagggatct ccgtgttttc tgcataactt agagatgata
     9481 caaatagccc tgctatcaac agcaaaatgc agcgaaactt ccctgccata gtcttccatc
     9541 cgtggaacgc aatttgttat aagaaaggct tcacatcacc tacgccttca cgcaccacga
     9601 ccggcgtatc atcggtaaga tcaataaccg ttgtcggttt ctgcccgaga tagccaccgt
     9661 gaataatcaa atctacctgt ttttccagac ggtctttaat ttcttcggga tcggattcgg
     9721 taaattcgct gcctggcagc attagcgaag tggaaagcat cggttcgccc agtgcttcaa
     9781 gcaacgcctg cgcaatcggg ttagaaggca cacgcatacc gatggttttg cgtttttcct
     9841 gcaacagacg gcgtggcact tccttcgtcc ccttcaggat gaaggtatag ttgcccggcg
     9901 tgttgttttt cattaaacga aacgcaacgt tatcaacaaa tgaataggtc gagagttcag
     9961 aaagatcgcg acacatcagg gtaaagttgt gaccgtccgg cagctggcga atacgacaaa
    10021 tacgctccat cgcgttttta tcttcaattt tacagccgag cgcatagccg gaatcagttg
    10081 gataaacaat caccccgcct ttacgcacga tctccaccgc ctggttgatc agacgttgct
    10141 gtgggttatc aggatgaata taaaaaaact ggctcataat tccctctctg tggtgttctg
    10201 cggctgttcc catagctgcc agacgccttc aacgcctgcg ggcagccaga gtttacgacc
    10261 tagttcgatc cacgggcatg gctgatgaaa atcagatcct tgtgatgccc ataaatgatg
    10321 ctgacgcgca agggccgcca gctgggtacg ttcattgggc gattgctggc actgcgcgac
    10381 ttccatcgcg tcaccgtggt gttcggcaaa atgcgcaacc aatcttttca gccatttagc
    10441 actaagattg taccgcccag gatgagctag caccgcctta ccgccagaat gatgaatgac
    10501 atcaatagct tgttctattg tacaccactg tggcggaacg tatccggttt tcccgcgcgc
    10561 cagatatttt ttaaagacat ccgccattga actggctttg ccgcacccca caaggaaacg
    10621 cgcaaaatga ccgcgcgtca ccgcaccgcc ctgcgccagt cgtcgcgcgc cttccagtgc
    10681 ccctggtatt tgcgcttttt caagtctttc ggcaatcagt tgcgcccgct gattgcgccg
    10741 ttctgtctgc tgcgcgagga actcacacat tagcggatga gtaatatcaa tattcagccc
    10801 gacaatatga atttcatgat tttcccagac cgtggaaatt tccacgccgg gaataagatt
    10861 cagcgccagt cccgaacgtg aaatttcctc tcttgctggc acgattgcag ctgtgttgtc
    10921 atggtcggtg atcgccaggg taccgacgcg catctcgact gcacggtgca ccaatacttc
    10981 tggcgtcagg cagccatcgg aagctgtggt atggctgtgc aggtcgtaaa tcactgcgta
    11041 attcgtgtcg ctcaaggcgc actcccgttc tggataatgt tttttgcgcc gacatcataa
    11101 cagttctggc aaatattctg aaatcagctg ttgacaatta atcatcgaac tagttaacta
    11161 gtacgcaagt tcacgtaaaa agggtatcga caatgaaagc aattttcgta ctgaaagctt
    11221 ggtggcgcac ttcctgaaac gggcagtgta ttcaccatgc gtaaagcaat cagataccca
    11281 gcccgcctaa tgagcgggct tttttttgaa caaaattaga gaataacaat gcaaacacaa
    11341 aaaccgactc tcgaactgct aacctgcgaa ggcgcttatc gcgacaaccc gactgcgctt
    11401 tttcaccagt tgtgtgggaa tcgtccggca acgctgctgc tggaatccgc agatatcgac
    11461 agcaaagatg atttaaaaag cctgctactg gtagacagtg cgctgcgcat tacagcatta
    11521 ggtgacactg tcacaattca ggcactttcc ggcaacggcg aagccctgct ggcactactg
    11581 gataacgccc tgcctgcggg tgtggaaaat gaacaatcac caaactgccg tgtgctgcgc
    11641 ttcccccctg tcagtccact gctggatgaa gacgcccgct tatgctccct ttcggttttt
    11701 gacgctttcc gtttattgca gaatctgttg aatgtaccga aggaagaacg agaagcaatg
    11761 ttcttcggcg gcctgttctc ttatgacctt gtggcgggat ttgaagattt accgcaactg
    11821 tcagcggaaa ataactgccc tgatttctgt ttttatctcg ctgaaacgct gatggtgatt
    11881 gaccatcaga aaaaaaacac ccgcattcag gccagcctgt ttgctccgaa tgaagaagaa
    11941 aaacaacgtc tcactgctcg cctgaacgat cttcgccagc agctgaccga agccgcgccg
    12001 ccgctgccgg tggtttccgt gccgcatatg cgttgtgaat gtaatcagag cgatgaagag
    12061 ttcggtggcg tagtgcgttt gttgcaaaaa gcgattcgcg ctggagaaat tttccaggtg
    12121 gtgccatctc gccgtttctc tctgccctgc ccgtcaccgc tggcggccta ttacgtgctg
    12181 aaaaagagta atcccagccc gtacatgttt tttatgcagg ataatgattt caccctgttt
    12241 ggcgcgtcgc cggaaagttc gctcaagtat gacgccacca gccgccagat tgagatctac
    12301 ccgattgccg gaacacgtcc acgcggtcgt cgtgccgatg gctcgctgga cagagacctc
    12361 gacagccgca tcgaactgga aatgcgtacc gatcataaag agctttctga acatctgatg
    12421 ctggtggatc tcgcccgtaa tgatctggca cgcatttgca cccccggcag cagctacgtc
    12481 gccgatctta ccaaagttga ccgttactct tacgtgatgc acctggtctc ccgcgtggtc
    12541 ggtgagctgc gccacgatct cgacgccctg cacgcttacc gcgcctgtat gaatatgggg
    12601 acgttaagcg gtgcgccgaa agtacgcgcc atgcagttaa ttgccgaggc ggaaggtcgt
    12661 cgccgcggca gctacggcgg cgcggtaggt tatttcaccg cgcatggcga tctcgacacc
    12721 tgcattgtga tccgctcggc gctggtggaa aacggtatcg ccaccgtgca agccggtgct
    12781 ggcgtagtcc ttgattctat tccgcagtcg gaagccgacg aaacccgtaa taaagcccgc
    12841 gctgtactgc gcgctattgc caccgcgcat catgcacagg agactttctg atggctgaca
    12901 ttctgctgct cgataatatc gactctttta cgtacaacct ggcagatcag ttgcgcagca
    12961 atgggcataa tgtggtgatt taccgcaacc atattccggc gcagacctta attgaacgcc
    13021 tggcgacgat gagcaatccg atactgatgc tttctcctgg ccccggtgtg ccgagcgaag
    13081 ccggttgtat gccggaactc ctcacccgct tgcgtggcaa gctgcccatt attggcattt
    13141 gcctcggaca tcaggcaatt gtcgaagctt acgggggcta tgtcggtcag gcgggcgaaa
    13201 ttcttcacgg taaagcgtcg agcattgaac atgacggtca ggcgatgttt gccggattaa
    13261 caaacccgct gccggtggcg cgttatcact cgctggttgg cagtaacatt ccggccggtt
    13321 taaccatcaa cgcccatttt aatggcatgg tgatggcagt acgtcaccat gcggatcgcg
    13381 tttgtggatt ccagttccat ccggaatcca ttctcaccac ccagggcgct cgcctgctgg
    13441 aacaaacgct ggcctgggcg cagcagaaac tagagccagc caacacgctg caaccgattc
    13501 tggaaaaact gtatcaggcg cagacgctta gccaacaaga aagccaccag ctgttttcag
    13561 cggtggtgcg tggcgagctg aagccggaac aactggcggc ggcgctggtg agcatgaaaa
    13621 ttcgcggtga gcacccgaac gagatcgccg gggcagcaac cgcgctactg gaaaacgcag
    13681 cgccgttccc gcgcccggat tatctgtttg ctgatatcgt cggtactggc ggtgacggca
    13741 gcaacagtat caatatttct accgccagtg cgtttgtcgc cgcggcctgt gggctgaaag
    13801 tggcgaaaca cggcaaccgt agcgtctcca gtaaatctgg ttcgtccgat ctgctggcgg
    13861 cgttcggtat taatcttgat atgaacgccg ataaatcgcg ccaggcgctg gatgagttag
    13921 gtgtttgttt cctctttgcg ccgaagtatc acaccggatt ccgccacgcg atgccggttc
    13981 gccagcaact gaaaacccgc accctgttca atgtgctggg gccattgatt aacccggcgc
    14041 atccgccgct ggcgttaatt ggtgtttata gtccggaact ggtgctgccg attgccgaaa
    14101 ccttgcgcgt gctggggtat caacgcgcgg cggtggtgca cagcggcggg atggatgaag
    14161 tttcattaca cgcgccgaca atcgttgccg agctgcatga cggcgaaatt aagagctatc
    14221 aattgaccgc tgaagatttt ggcctgactc cctaccacca ggagcaactg gcaggcggaa
    14281 caccggaaga aaaccgtgac attttaacac gcttgttaca aggtaaaggc gacgccgccc
    14341 atgaagcagc cgtcgctgcg aacgtcgcca tgttaatgcg cctgcatggc catgaagatc
    14401 tgcaagccaa tgcgcaaacc gttcttgagg tactgcgcag tggttccgct tacgacagag
    14461 ttaccgcgct ggcggcacga gggtaaatga tgcaaaccgt tttagcgaaa atcgtcgcag
    14521 acaaggcgat ttgggtagaa acccgcaaac agcagcaacc gctggccagt tttcagaatg
    14581 aggttcagcc gagcacgcga catttttatg atgcgctaca gggtgcacgc acggcgttta
    14641 ttctggagtg caagaaagcg tcgccgtcaa aaggcgtgat ccgtgatgat ttcgatccgg
    14701 cacgcattgc ctccatttat aaacattacg cttcggcaat ttcggtgctg actgatgaga
    14761 aatattttca ggggagcttt gatttcctcc ccatcgtcag ccaaatcgcc ccgcagccga
    14821 ttttatgtaa agacttcatt atcgaccctt accagatcta tctggcgcgc tattaccagg
    14881 ccgatgcctg cttattaatg ctttcagtac tggatgacga ccaatatcgc cagcttgcag
    14941 ccgtcgctca cagtctggag atgggggtgc tgaccgaagt cagtaatgaa gaggaactgg
    15001 agcgcgccat tgcattgggg gcaaaggtcg ttggcatcaa caaccgcgat ctgcgtgatt
    15061 tgtcgattga tctcaaccgt acccgcgagc ttgcgccgaa actggggcac aacgtgacgg
    15121 taatcagcga atccggcatc aatacttacg ctcaggtgcg cgagttaagc cacttcgcta
    15181 acggttttct gattggttcg gcgttgatgg cccatgacga tttgcacgcc gccgtgcgcc
    15241 gggtgttgct gggtgagaat aaagtatgtg gtctgacgcg tgggcaagat gctaaagcag
    15301 cttatgacgc gggcgcgatt tacggtgggt tgattttggt tgcgacatca ccgcgttgcg
    15361 tcaacgttga acaggcgcag gaagtgatgg ctgcggcacc gttgcagtat gttggcgtgt
    15421 tccgcaatca cgatattgcc gatgtggtgg acaaagctaa ggtgttatcg ctggcggcag
    15481 tgcaactgca tggtaatgaa gatcagctgt atatcgatac gctgcgtgaa gctctgccag
    15541 cacacgttgc catctggaaa gcattaagcg tcggtgaaac cctgcccgcc cgcgagcttc
    15601 agcacgttga taaatatgtt ttagacaacg gccagggtgg aagcgggcaa cgtttcgact
    15661 ggtcactatt aaatggtcaa tcgcttggca acgttctgct ggcggggggc ttaggcgcag
    15721 ataactgcgt ggaagcggca caaaccggct gcgccggact tgattttaat tctgctgtag
    15781 agtcgcaacc gggcatcaaa gacgcacgtc ttttggcctc ggttttccag acgctgcgcg
    15841 catattaagg aaaggaacaa tgacaacatt acttaacccc tattttggtg agtttggcgg
    15901 catgtacgtg ccacaaatcc tgatgcctgc tctgcgccag ctggaagaag cttttgtcag
    15961 cgcgcaaaaa gatcctgaat ttcaggccca gttcaacgac ctactgaaaa actatgccgg
    16021 gcgtccaacc gcactgacca aatgccagaa cattacggct ggcacgaaca ccacgctgta
    16081 tctgaagcgc gaagatttgc tgcacggcgg cgcgcataaa actaaccagg tgctcggtca
    16141 ggctttactg gcgaagcgga tgggtaaaac cgaaatcatc gccgaaaccg gtgccggtca
    16201 gcatggcgtg gcgtcggccc ttgccagcgc cctgctcggc ctgaaatgcc gtatttatat
    16261 gggtgccaaa gacgttgaac gccagtcgcc taacgttttt cgtatgcgct taatgggtgc
    16321 ggaagtgatc ccggtgcata gcggttccgc gacgctgaaa gatgcctgta acgaggcgct
    16381 gcgcgactgg tccggtagtt acgaaaccgc gcactatatg ctgggcaccg cagctggccc
    16441 gcatccttat ccgaccattg tgcgtgagtt tcagcggatg attggcgaag aaaccaaagc
    16501 gcagattctg gaaagagaag gtcgcctgcc ggatgccgtt atcgcctgtg ttggcggcgg
    16561 ttcgaatgcc atcggcatgt ttgctgattt catcaatgaa accaacgtcg gcctgattgg
    16621 tgtggagcca ggtggtcacg gtatcgaaac tggcgagcac ggcgcaccgc taaaacatgg
    16681 tcgcgtgggt atctatttcg gtatgaaagc gccgatgatg caaaccgaag acgggcagat
    16741 tgaagaatct tactccatct ccgccggatt ggatttcccg tctgtcggcc cacaacacgc
    16801 gtatcttaac agcactggac gcgctgatta cgtgtctatt accgatgatg aagcccttga
    16861 agccttcaag acgctgtgcc tacacgaagg gatcatcccg gcgctggaat cctcccacgc
    16921 cctggcccat gcgctgaaaa tgatgcgcga aaacccggaa aaagagcagc tactggtggt
    16981 taacctttcc ggtcgcggcg ataaagacat cttcaccgtt cacgatattt tgaaagcacg
    17041 aggggaaatc tgatggaacg ctacgaatct ctgtttgccc agttgaagga gcgcaaagaa
    17101 ggcgcattcg ttcctttcgt cacgctcggg gatccgggca ttgagcagtc attgaaaatt
    17161 atcgatacgc taattgaagc cggtgctgac gcgctggagt taggcatccc cttctccgac
    17221 ccactggcag atggcccgac gattcaaaac gccactctgc gcgcctttgc ggcaggtgtg
    17281 actccggcac aatgttttga aatgctggca ctgattcgcc agaaacaccc gaccattccc
    17341 attggcctgt tgatgtatgc caatctggtg tttaacaaag gcattgatga gttttatgcc
    17401 cagtgcgaga aagtcggcgt cgattcggtg ctggttgccg atgtgccaat tgaagagtcc
    17461 gcgcccttcc gccaggccgc gttgcgccat aatgtcgcac ctatcttcat ctgcccgcca
    17521 aatgccgatg acgacctgct gcgccagata gcctcttacg gtcgtggtta cacctatttg
    17581 ctgtcacgag cgggcgtgac cggcgcagaa aaccgcgccg cgttacccct caatcatctg
    17641 gttgcgaagc tgaaagagta caacgctgca cctccattgc aggggtttgg tatttccacc
    17701 ccggatcagg taaaagcagc gattgatgca ggagctgcgg gcgcgatttc tggttcagcc
    17761 attgttaaaa tcatcgagca acatattaat gagccagaga aaatgctggc ggcactgaaa
    17821 gtttttgtac aaccgatgaa agtggcgacg cgcagttaat cccccagccg ccagttccgc
    17881 tggcggcatt ttaactttct ttaacgaagc cggaaaaatc ctaaattcat ttaatattta
    17941 tctttttacc gtttcgctta cccctgtcga a
//
LOCUS       DAKISA010000091        17690 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.091, whole genome shotgun sequence.
ACCESSION   DAKISA010000091 DAKISA010000000
VERSION     DAKISA010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17690
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.091"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            1..839
                     /locus_tag="OVW45_003156"
                     /pseudo
     CDS             1..839
                     /locus_tag="OVW45_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1453..1829)
                     /gene="rnpB"
                     /locus_tag="OVW45_003157"
     ncRNA           complement(1453..1829)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OVW45_003157"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1862..3007)
                     /gene="garK"
                     /locus_tag="OVW45_003158"
     CDS             complement(1862..3007)
                     /gene="garK"
                     /locus_tag="OVW45_003158"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCV5527541.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESSATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(3104..3994)
                     /gene="garR"
                     /locus_tag="OVW45_003159"
     CDS             complement(3104..3994)
                     /gene="garR"
                     /locus_tag="OVW45_003159"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCV5527542.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLLLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(4024..4794)
                     /gene="garL"
                     /locus_tag="OVW45_003160"
     CDS             complement(4024..4794)
                     /gene="garL"
                     /locus_tag="OVW45_003160"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HCV5527543.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(4810..6144)
                     /gene="garP"
                     /locus_tag="OVW45_003161"
     CDS             complement(4810..6144)
                     /gene="garP"
                     /locus_tag="OVW45_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCV5527544.1"
                     /translation="MILDTVDVKKKGVHTSYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGGIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     VSTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            6519..8090
                     /gene="garD"
                     /locus_tag="OVW45_003162"
     CDS             6519..8090
                     /gene="garD"
                     /locus_tag="OVW45_003162"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCV5527545.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGVQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEESIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            8239..8665
                     /locus_tag="OVW45_003163"
                     /pseudo
     CDS             8239..8665
                     /locus_tag="OVW45_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PrlF family
                     antitoxin"
     gene            8665..9129
                     /gene="yhaV"
                     /locus_tag="OVW45_003164"
     CDS             8665..9129
                     /gene="yhaV"
                     /locus_tag="OVW45_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HCV5527546.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTNAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(9184..9993)
                     /gene="agaR"
                     /locus_tag="OVW45_003165"
     CDS             complement(9184..9993)
                     /gene="agaR"
                     /locus_tag="OVW45_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HCV5527547.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            10242..11522
                     /gene="kbaZ"
                     /locus_tag="OVW45_003166"
     CDS             10242..11522
                     /gene="kbaZ"
                     /locus_tag="OVW45_003166"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HCV5527548.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGDAHETLSELAVTTPDAARATLEAHRHAFKKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKATALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            11545..12018
                     /gene="agaV"
                     /locus_tag="OVW45_003167"
     CDS             11545..12018
                     /gene="agaV"
                     /locus_tag="OVW45_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCV5527549.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            12029..12808
                     /locus_tag="OVW45_003168"
     CDS             12029..12808
                     /locus_tag="OVW45_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HCV5527550.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPSAQKEEFEDGI"
     gene            12798..13676
                     /locus_tag="OVW45_003169"
     CDS             12798..13676
                     /locus_tag="OVW45_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCV5527551.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQTDVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            13694..14128
                     /locus_tag="OVW45_003170"
     CDS             13694..14128
                     /locus_tag="OVW45_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCV5527552.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTCLVDELGRCHEECPVEEGI"
     gene            14125..15258
                     /gene="nagA"
                     /locus_tag="OVW45_003171"
     CDS             14125..15258
                     /gene="nagA"
                     /locus_tag="OVW45_003171"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCV5527553.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLCITDGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVFDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAAAWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            15609..16763
                     /locus_tag="OVW45_003172"
     CDS             15609..16763
                     /locus_tag="OVW45_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HCV5527554.1"
                     /translation="MPENYTPAAAATDTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYTPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDVIAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            16776..17636
                     /gene="kbaY"
                     /locus_tag="OVW45_003173"
     CDS             16776..17636
                     /gene="kbaY"
                     /locus_tag="OVW45_003173"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HCV5527555.1"
                     /translation="MSIISTKYLLQDAQANDYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KCFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
BASE COUNT         4418 a         4623 c         4399 g         4250 t
ORIGIN      
        1 aatatctaca ggcaatatca atgttaattg cccattaaaa aatatttaca atgaaatcag
       61 gaggttgaac gtaattttta gttgtgggac tggagatata gttgatctat cctctctgga
      121 cttacgtaat gtcgatttag attattacga ttttacagat aaacatatgg ctaatactat
      181 tttaaatcct tttaaattgg attcaacaaa ttttactaat gccaacatgt tccaggttaa
      241 ttttgttagt tcaacacaaa acgccacaat ctcctgggat tatttactaa aaataacgcc
      301 tgttttaata agcattagcg atatgtattc tgaagaaaaa atcaagtttg tcgaaagttg
      361 tttaaatgag cttggagaca ttaccgaaga acaattaaaa attatgagat ttgcaattat
      421 aaaatctata ccaagggcaa ctcttacaga taaattagaa aatgaattaa caaaagaaat
      481 atataaaagc tcatcgaaaa tcaacaattg cttgaacaga attaaattac cagagatgaa
      541 agaattctca tcagaaaaaa atatatgatt acatcgatat aatcattgaa gattatgaaa
      601 atatcaaaga aaatgcttat ctggtcgatc caaaaattaa ttacactata gatttaaaca
      661 tagaagactc tagctcagag gaattccttt ttgataatag cctcgagaaa gacgaaaatt
      721 cttcggacaa ttgctttgag gtcgaaaaat ataacgcata tgaagcatat aactcagaga
      781 atctatattt taccagagag gaatatacgt atgattacga ccttttaaat gcaatataga
      841 cataataact tcttacaaaa tctataatta ataccgtaga tattcattca tcttaccctg
      901 tgcaccaaaa gcaaaaactc actgacgcgg gtttgaaact gatggcaacc gcaaaaatgc
      961 ctgatgcgct acgcttatca ggcctacgcc atctctacaa tatattgaat ttgcgtgctt
     1021 ttgtaggcag gataaggcgt tcacgcagca tccggcatga ataaagcgca ctcaacaatc
     1081 tgaaacccgc cggaacggtt tattacgtac atcaggtgaa actgaccgat aagccgcttt
     1141 cttttgggta tagtgtcgtg gacagtcatt catctttctg cccctccaaa agcaaaaacc
     1201 cgccgaagcg ggtttttacg taaatcaggt gaaactgacc gataagccgc tttcttttgg
     1261 gtatagcgtc gtggacagtc attcatcttt ctgcccctcc aaaagcaaaa acccgccgaa
     1321 gcggtttttt acgtaaacca ggtgaaactg accgataagc cgctttcttt tgggtatagc
     1381 gtcgtggaca gtcattcatc tttccgctcc tccaaaagca aaaacccgcc gaagcgggtt
     1441 tttacgtaaa tcaggtgaaa ctgaccgata agccgggttc tgtcgtggac agtcattcat
     1501 ctaggccagc aatcgctcac tggctcaagc agcctacccg ggttcagtac gggccgtacc
     1561 ttatgaaccc ctatttggcc ttgctccggg tggagtttac cgtgccacgg actgttacca
     1621 gccgcgcggt gcgctcttac cgcacccttt cacccttacc tgatcccgct tgcgcgggcc
     1681 atcggcggtt tgctctctgt tgcactggtc gtgggtttcc cccccaggcg ttacctggca
     1741 ccctgcccta tggagcccgg actttcctcc cctccgcccg tctcccccga agaggacgac
     1801 gacgaagcgg cgactgtctg gtcagcttcg gcgcgcagta tagagggttt gcgcgccctt
     1861 gtcaccccgc gttgcgcatt ccaatcgcca gtgtcgcggc gatattacgt gaagcacggc
     1921 agatattgtc ataagccccg cggaatgctt cgtccaacgt acctatgctg gtcaatacgc
     1981 tgaagaccgc atcgatgcca tgctgatgta caacgccaac atcatcggtc aggctacccg
     2041 caatgccaat caccggttta tggtacttct tcgccacgtt tgcaacgcca atcggtacct
     2101 tgccgtgaat actctggctg tcaatacgcc cttcaccggt gatcaccagc gtacaatcgt
     2161 gaatatgttc ttccagattc agcgccgtag tgacgatttc aataccactt ttcagttccg
     2221 caccaagaaa cgccatcagc gccgcgccca taccgcccgc agctcctgcg ccggggacat
     2281 ctttcacatc aacatgcagc gcttttttaa tgacatcggc atagtgagag aggttattgt
     2341 ccagctcaac aatcatcgct tcactggctc ccttttgtgg gccaaagatg cgcgatgcgc
     2401 cgttatcgcc caccagcgga ttggtgacat cacaagcgac gcgaatgacg caatctttta
     2461 agcgcggatc gaggccggaa atatcaatat cattcagagt attaagacta ccgccgccaa
     2521 aaccaatttc attgccgttg gcgtcgcata atttcgcccc cagcgcctgt accatgcctg
     2581 cgccgccatc atttgtagcg ctgccgccaa tgccgataat aatgtttgtt gcactgctct
     2641 ccagcgcctg caggattaac tcgcctgtgc cgcgtgaagt ggttacgagt ggatcgcgtt
     2701 tttccgcagg taccagctcc agcccactgg ccgccgccat ttcaataaac gcggttttgc
     2761 catcgccgga gatcccccaa ctggcattca ctttctcgcc cagcggccct gtaacccagg
     2821 cgtgacgttc agccccctgg gtggctgcaa tcatcgcttc caccgttcct tcaccaccgt
     2881 cggcaaccgg aacagaaaca tactgtgcat caggaaaaat ttcccgaaat cctttttcta
     2941 tcgcctgcgc aacctcgctg gcagataaac tttctttata agagtctggg gcgattacga
     3001 ttttcatacc tatgcctgtt accacatgac gccggagggc gtttctctta ttcggcctag
     3061 attccaggcc cggattgcaa tacaccatcc gggcacgacg tcattaacga gtaacttcga
     3121 ctttcgccag tttttcgtag tagcacgcca gggcgctatg atccgccgtt cctaaaccat
     3181 ctgcacgcag tgcctgcatc atctccataa ccgcagctgt gagcagcagt tgtgcgccga
     3241 cgccgtgaga agtatccagc gcattcgcca gatccttaat atgcagatca atgcggaagc
     3301 ccggcttgaa gttgcggtcc atcaccatcg gcgctttggc atccagcacg gtactgcccg
     3361 ccagcccacc gcgaattgcc tgataaacca ggtccgggtt aacgcccgct ttggttgcca
     3421 gcgttaacgc ttctgacatc gcagcaatat tcagcgccac aatgacctga tttgccagtt
     3481 tggtgacgtt acctgcaccg atttccccgg tatgcaccac ggaacccgcc atcgctttca
     3541 tcaaatcata gtatttgtcg aaaatagcct tgtcgccgcc caccatcact gacagcgtac
     3601 cgtcgatggc tttcggttca ccgccgctca ccggagcatc cagcatatca atgcctttcg
     3661 ctttcagcgc ttcgctgatt tcacggcttg ccagcggtgc gatagaactc atatcgatca
     3721 ataccgtacc tggcttcgcg ccttcaataa tgccattctc acccagcgcc acctctttca
     3781 catgagggga gtttggcagc atggttatga tgacgtcgca ctgttcagcg atcgctttag
     3841 ccgtagacgc tgtttctgca cctgcagcaa tcacgtcagc aatagcttct gggttacggt
     3901 cagcaaccac cagcgagtaa cctgctttca gaaggttttt actcattggt ttacccataa
     3961 tccccaggcc aataaaacca actttcatag tcatatcaac catctctctt gttgcggtgg
     4021 tggttatttt ttaaaggtat cagccagttt ctgagtggca gagcggaaga cgccaagatc
     4081 gctgccgaca gccacaaacg tcgcgcccca ttccagataa cgacgcgcat cggcttcgac
     4141 cggcgcgaga ataccgctgg gtttgccgtg cgcgctggca cggttaaaaa tatgctgaat
     4201 tgctttttgt acatccggat gcgaggcatt gccgagatgg cccaatgccg cagccagatc
     4261 gctggggccg acgaagatac cgtctacacc ttcggtagcg gcaatagcat cgacgttatc
     4321 tacgccctgc tgactttcta tctggaccag aatagtaatg ttcttgttcg actgagcgaa
     4381 ataatccgcc acggtgccaa acatattggc gcggtgagaa acggagacgc cgcgaatgcc
     4441 ttccggcggg taacgagttg atgccaccgc ctgctctgct tcctcttttg tttctacaaa
     4501 aggaatcagg aagttataga aaccgatatc cagaagacgc ttaataatta ccggctcgtt
     4561 ggtcggcact cgcactactg gcgcgctggc gctgcctttc agggccatta actgcggaat
     4621 aaacgtggaa atatcgtttg gcgcatgttc gccatccagc accagccagt caaacccagc
     4681 caaaccaaga acttcagtgc taatcgggtt agagagtgct gaccagcaac caatttgtac
     4741 ctgtttcgca gccagtgcgg ctttgaattt attcgggaaa acatcgttat tcatcgctta
     4801 tacctttgct tatttctgca attccatacg tttaatgtcg ccaactacga agaggtagca
     4861 gaccatcgcc atcagcgctg aacatcccac gaaaaccagt gctgcattga aggagtgcag
     4921 ttcacttacc aggtagccaa tcaccagtgg agtgacaatg gaggcaacat tgccaaagac
     4981 gttaaagacg ccgccacaga ggccaacaat ctctttcggc gcggtgtcag aaatcaccgg
     5041 ccagcccaga gcaccaaatc ctttgccaaa aaaagccagc gccatcagca tgaccaccag
     5101 cgtggtgttg ttggtgtagt tacataagat gatggtggaa accagcaaca ttcccagcac
     5161 aatcggtagc ttacgtgcca gggtcaggga taaaccgcgt ttgatcagat aatccgagaa
     5221 gacacctccc agcacgccgc ccgcaaaacc acacagtgcc ggaatagagg cgaccagacc
     5281 cactttcaga atcgacatgc ctttttcctg caccagataa atcgggaacc aggtgaggaa
     5341 gaaccaggtg atggtgttga taaaatattg tccgaaaaat acgcccaaca tcatgcggtt
     5401 agagagcaat tgcttgatgt aatgcagttt gggaccgctt gctgccgcgc tgcccggctt
     5461 tttgtggtcc atatcgacca ccgcgccatt ttcagagata aacttcagct cttccgcaga
     5521 catacgtggg tgatctgtcg ggttatgaat caacttgatc cacagcgccg tcagcacaaa
     5581 accaatcccc cccataacgg tgaagacgtg ctcccagccc caagcgaaag tcagccagcc
     5641 aagcagcggc gaaaagagcg ccagcgagaa atattgcgcc gagttaaaga tggcggaggc
     5701 agtaccacgt tctttcgtcg ggaaccaggc ggcgacaata cgggcgttcg ccgggaatga
     5761 tggcgcttcc gagaacccca gcataaagcg cataaagaac atggagatcc ctgcccaggc
     5821 cagcgggaac atatcaacaa agccttgcag gaaggtgaac agcgaccaga aaaagaggct
     5881 gtaggtgtaa acttttttcg agccaaactt atcaagcaac cagccgccgg ggatttgcat
     5941 cagcaagtag gcccagccaa aagcagagaa gatgtaaccc atcgaaaccg cacttaactg
     6001 caactctttt gccacttcgg taccagcaat agacagcgtt gcacgatcgg cgtagttaac
     6061 ggcggtaaca ataaaaataa tcagtaatat taaatagctg gtatgcacgc ctttcttttt
     6121 tacgtcaacg gtgtccagaa tcattttatt tacctcgggt acttatgctg atttttatta
     6181 ttatggggaa ggtgttattt atgagtttta tttatgccgt tacgacaatg aactcgggaa
     6241 ttagtataag cagcgggaga ataataatca ttgtgcaaat gctaatttaa tcagtactat
     6301 ttaaatatta ttttgagcat atgcacataa ggttgcgcgc taaagcacag atttgcgctt
     6361 taccttaccg ggcggcactg caatccctga aatgattgac attgatcaca tttctgcgtt
     6421 taaactcctg acattcttat ttcacccaat gaagtcattt atttttaaat gagaccagat
     6481 cctcatttta ataacccctg gctggagaat attgcacaat ggccaacatc gaaatcagac
     6541 aagaaacgcc aactgcgttt tatataaaag ttcacgacac agataatgtg gcaattattg
     6601 ttaatgataa tggcctgaaa gcaggaacgc gttttccgga tgggctggaa ttaattgaac
     6661 atattcccca ggggcataaa gtcgcattgc tggacattcc agctaatggt gaaattattc
     6721 gttatggcga agtgattggt tacgccgtgc gcgcaatccc acgcggaagc tggatcgacg
     6781 aatcaatggt tgtactaccg gaagcgccgc cgttacacac gctgccactg gcaaccaaag
     6841 tcccggcccc cttaccgccg ctggaaggat acacctttga gggctatcgc aatgccgatg
     6901 gcagcgtggg caccaaaaac ctgctcggca tcaccaccag cgtccactgt gtggcaggcg
     6961 tggtggacta tgtagtaaaa atcattgaac gcgatctgct accgaaatac ccgaacgtcg
     7021 atggcgtggt ggggctgaat catttgtacg gctgtggcgt ggcgattaac gcaccggcgg
     7081 cagttgtgcc tattcgtacc atccacaata tttcgctgaa ccctaacttt ggcggcgaag
     7141 taatggtgat tggtctgggt tgtgaaaagt tgcagcctga gcgcctgctg gtcggcacgg
     7201 atgatgtgca agctattcca gtagaaagcg ccagcattgt cagtttgcag gatgaaaagc
     7261 atgtcggctt tcagtccatg gtcgaggata ttttgcaggt cgccgaacgc catctacaaa
     7321 aactgaatca acggcagcga gaaacctgcc cggcttcaga actggtcgtt ggtgtgcagt
     7381 gcggtggcag cgatgcgttt tctggtgtaa cggcaaaccc ggcggttggc tatgcgtctg
     7441 atctactggt gcgctgcggc gcaacggtga tgttttcaga agtaacggaa gtgcgtgacg
     7501 cgatccatct gctgacacca cgcgcagtga acgaagaggt cggcaaacgg ctgctggagg
     7561 agatggagtg gtacgataac tatctcaata tgggaaaaac cgaccgcagc gccaaccctt
     7621 cgccgggcaa caagaaaggc ggtctggcaa atgtggtgga gaaggcactc ggctccattg
     7681 ctaaatcggg taaaagtgca attgttgaag tgctgtcgcc cggtcaacgc ccgactaaac
     7741 gcggattaat ttacgccgcg acgccagcca gcgattttgt ctgtggcacg caacaggtgg
     7801 cttcgggtat caccgtacaa gtgtttacga ccggccgtgg tacgccgtac ggcctgatgg
     7861 cggtacccgt aattaaaatg gcaacccgca ccgagctggc gaaccgctgg tttgatttaa
     7921 tggatattaa cgcgggcact atcgctaccg gcgaagagag cattgaagag gtgggctgga
     7981 agttgttcca ctttattctc gacgtcgcca gcgggaagaa gaaaaccttc tcggatcaat
     8041 gggggctgca taaccagctg gcggtgttta acccggcacc ggtgacctga tttcttttgg
     8101 aacgtccctc gcaaaacatg gccttagtgc catgttttta ttgtttaaag cccccacgtc
     8161 cattaataat gcatttgcat taccttagtt caagcttata atttgagcag aaaacaggct
     8221 gtaaaaggac aatgaatcat gcccgctaat gctcgctctc acgctgtact gaccactgaa
     8281 tcaaaggtca cgatacgcgg acaaacaact atccccgcgc cagtgcgtga ggccttaaaa
     8341 ctgaagccag gctaggacag cattcattac gaaattctgc ctggtgggca agtatttatg
     8401 tgccgactgg gagatgaaca ggaggatcat actatgaatg catttttgcg ttttctggat
     8461 acagatatcc agaacaaccc gcaaaaaact cgtccattca acattcaaca ttcaacattc
     8521 aacattcaac attcaacatt caacattcaa cattcaacat tcaacattca acattcaaca
     8581 ttcaacattc aacattcaac aaggaaagaa acttgtcgct ggcatggacg tcaacattga
     8641 tgatgagatt ggcgacgacg aataatggat tttccacaaa gggttaatgg ttgggcgcta
     8701 tatgctcatc cctgttttca ggaaacctac gacgctttag ttgccgaagt cgagacatta
     8761 aagggaaaag atcctgaaaa ttatcagaga aaagccgcca caaagttatt ggcggtagtc
     8821 cataaagtga ttgaggagca tatcacggtc aatccatcat caccggcatt ccgtcatggc
     8881 aagtcgttag gctctgggaa aaataaagac tggtcacggg taaaatttgg tgcaggtcgt
     8941 tatcgtctct tctttcgtta tagtgaaaaa gagaaagtca tcattctggg atggatgaac
     9001 gatgaaaaca ctctgcgcac ctacggtaaa aaaacaaatg cctataccgt attcagcaaa
     9061 atgttaaaaa gaggacatcc tcctgccgac tgggaaaccc tcacccgaga gacagaagaa
     9121 acccattgat ggtgttcaca atgcccgccg aacgcattcg tgttgaccag cgggcaccct
     9181 ttctcactcc ccgaccagaa tcacttcaac cccagccttt cgcagtcctt ccagactatc
     9241 cgcaggaatg ccttcatcaa caatgatcat gtcgatacgt tgagtatcaa tgatcttatg
     9301 taaactggag cggttgaact tactggaatc ggtgaccacg atgatccgtt ccgcaacttc
     9361 gcacatccgg cggtttaaac gggcttcatc ttcattatgt gtgctgacgc cgcgctccag
     9421 atcgatcgca tctacaccaa gaaacagcat atcgaagtgg taattttgca gcgattgctc
     9481 agcctgatcg ccgtaaaaag attgcgactg acggcgcaaa tgcccgccgg tcatcagcag
     9541 ctcaacgcct tcggcttcca gcaacgcatt agccacgttc ataccgttgg tcatcgcaat
     9601 tacgtcagtg tgcttgcgca tcagacgagc aatctcaaaa gtggtggtcc cggaatcgag
     9661 gatcacccga tgacctggct gaatcaactc aacggcagct ttcgcaacgc tgcgtttcat
     9721 cgcggtgttc agtgcgcttt tatcttccac tgatggctcg actgacggcg tcgtgctatc
     9781 gcagatcaac gcgccaccat aggcacgcac agcgatcccc tgcttttcca gaaacgccag
     9841 atcgttgcgg atcgtcacag tagatacgcc atacaatgcc gacagatcgt taacctgcac
     9901 actcccttgc tgtcgcaggc gctgaatgat ctgttctcgt cgctcgctgg tgcctgtcac
     9961 tcgcttctca cctgaagcgt cggtattact catagtaagt cctttcgtaa aactttcgtt
    10021 tcatttcgtt ttgcctatta acgcctttct attaagcaaa tgcaagccca ccttgcccat
    10081 tgacgcaagc tactctcgtt tcagtaactt tcattatgtt tcttttgtga atcagatcag
    10141 aaaactatta tctttcgttt tatttttatc tcaccatggc gcagcatcaa ctgaaacaaa
    10201 acgaaagatt aatatcgcag taatctgaac tggagaggaa agtgaaacat ctgacagaaa
    10261 tggtgagaca gcacaaagcg ggcaaaacaa atggaattta tgccgtttgt tccgcacatc
    10321 cgctggtgct ggaggctgca atccgctacg ccagtgcaaa ccaaacgccg ttactgattg
    10381 aagcaacctc caatcaggta gaccagttcg gcggttatac cggaatgacg cccgccgatt
    10441 ttcgcggctt tgtttgtcag ctcgccgact cgttgaattt cccgcaggat gcgttgattc
    10501 tgggtggtga ccatctgggg ccaaaccgct ggcaaaacct gccagccgct caggcaatgg
    10561 ccaatgccga tgatttgatt aaaagctacg ttgcggcagg attcaaaaaa atccaccttg
    10621 attgcagcat gtcctgtcag gacgatccaa ttcccttaac tgatgacatc gtggctgaac
    10681 gcgccgcccg tctggcgaaa gtggcggaag aaacctgtcg cgaacacttt ggcgaagccg
    10741 atctggagta tgtcattggt accgaagtgc cggtacctgg cgacgcgcat gaaaccttaa
    10801 gcgagctggc ggtcaccacg ccggatgccg cccgcgccac gctggaagcc catcgtcacg
    10861 cctttaaaaa gcagggtctg aatgccatct ggccacgcat cattgccctg gtggttcaac
    10921 ccggcgtcga attcgatcac accaacgtta ttgattatca gcccgccaaa gcgaccgcct
    10981 taagccagat ggtcgaaaac tacgaaacgc tgatattcga agcgcactct acagattatc
    11041 aaacgccgca atcgctgcgc cagctggtga ttgatcactt tgccattctg aaagttggcc
    11101 cggcgctgac cttcgccctg cgtgaagccc tgttttctct ggcggcgatt gaagaagaac
    11161 tggtgccagc aaaagcctgt tccggtctgc gtcaggtgct ggaaaacgtg atgctcgacc
    11221 ggccggaata ctggcaaagc cactaccacg gtgacggcaa cgcgcgtcgt ctggcgcgtg
    11281 gttatagcta ctcggatcgc gtgcgctatt actggccgga cagccagatt gatgacgctt
    11341 tcgctcatct ggttcgtaat ctggcggatt caccaattcc gctaccgctc atcagccagt
    11401 atctgccgct gcaatacgtg aaagttcgct ccggcgagct gcaaccgacg ccacgggaac
    11461 tcattatcaa ccatattcag gacatcctgg cgcagtacca cacagcctgt gaaggccaat
    11521 aagcaaaaca aagaggaaca cgctatgcca aatattgttt taagccggat tgatgaacgc
    11581 ttgattcacg gtcaggtcgg cgttcagtgg gtcggatttg cgggggcaaa tctggtgctg
    11641 gtagccaacg atgaggttgc cgaagatccg gtacaacaaa acctgatgga aatggtactg
    11701 gcagaaggga tcgccgtacg tttctggacg ctgcaaaaag ttatcgacaa cattcatcgc
    11761 gccgccgatc gacagaaaat cctgctggtt tgtaaaacac ccgccgattt cctgacgctg
    11821 gtgaaaggtg gcgttccggt gaatcgcatt aacgttggca atatgcacta cgccaatggc
    11881 aaacaacaaa tcgccaaaac ggtttctgtg gatgcgggcg atatcgcagc atttaacgac
    11941 ctgaaagccg ctggggtgga atgcttcgtt cagggcgtcc cgacagagcc tgctgtggac
    12001 ctctttaaat tactttgagg gattcatcat ggaaatcagt ctgttgcagg cattcgcgtt
    12061 gggcattatc gcctttatcg ctggcctgga tatgtttaac ggcctaaccc atatgcaccg
    12121 cccggtggtc ctcggcccgt tggtcgggct ggtacttggc gatctgcata ccggaatttt
    12181 aaccggcggt acgctggaac tggtgtggat ggggctggcc ccgctggcgg gcgcacagcc
    12241 gcctaacgtg attatcggta ctatcgtcgg cacggcgttt gccattacta ctggcgtgaa
    12301 acccgatgtc gcagtcggtg tcgccgtacc tttcgctgtc gcagtacaga tggggattac
    12361 cttcctgttc tcggtgatgt ctggcgtgat gtctcgctgc gaccggatgg cggagaacgc
    12421 cgatactcgc ggcattgaac gcgtgaacta tctggcactg ctggcactcg gcactttcta
    12481 ttttctctgc gctttcctgc ctatctactt cggcgcggaa catgcaaaaa ccatcattga
    12541 tgtcctgccg caacgattaa tcgacggcct cggcgtcgcg ggcggcatca tgccagcaat
    12601 cggttttgcc gtgctgctga aaatcatgat gaaaaacgtc tacatcccct acttcatcct
    12661 gggctttgtt gccgcagcct ggctcaagtt accggtgctg gctattgctg ccgctgcgct
    12721 ggcgatggcg ctgatcgacc tgctgcgtaa atctcctgaa ccgactcaac cttcggcaca
    12781 gaaagaggaa tttgaagatg gcatctaatc aaaccaccct gccgaacgtc agtgaaaacg
    12841 aagaaacatt gctgactggc gtcaatgaaa acgtgtatga agatcagagc attggcgcgg
    12901 agctgacgaa aaaagatatc aaccgcgtcg cctggcgttc catgctgtta caagcgtctt
    12961 ttaactacga acgtatgcag gcttctggct ggttgtacgg tctgctgcct gctctgaaaa
    13021 agatccacac taataaacgc gacctggcgc gcgccatgaa ggggcatatg ggtttcttca
    13081 atacccatcc gtttctggtg acgtttgtta tcggcattat ccttgcgatg gagcgttcta
    13141 agcaggacgt taacagtatt cagagcacca aaattgccgt cggtgcgccg cttggcggga
    13201 ttggcgatgc aatgttctgg ctgacgctac tgccgatttg tggcgggata ggcgcaagcc
    13261 tcgcgctaca aggctctatt cttggcgcag tcgtctttat tgtgctgttc aacgtggtac
    13321 acctggggct gcgttttggt ctggcgcatt atgcttaccg catgggcgtg gcggcgattc
    13381 cactcattaa agcgaatacc aaaaaagtcg gccatgctgc gtcaatcgtt ggaatgacgg
    13441 taatcggcgc actggtggca acctatgttc gtttaagcac cacgctggaa atcacagcgg
    13501 gcgacgcagt ggttaagtta cagactgatg ttattgacaa actgatgcct gccttcttac
    13561 cgctggtcta caccctgacc atgttctggc tggtacgccg cggctggagt ccgctgcgcc
    13621 tgattgcagt gaccgtggtt ctcggcatcg tcggtaaatt ctgccatttc ctttaaatac
    13681 aatgaggtat tagatgttaa gtattatttt gacagggcat ggcgggtttg ccagtggcat
    13741 ggaaaaagca atgaagcaaa tcctcggcga tcaatcgcag tttattgcca tcgattttcc
    13801 cgaaacctca agcaccgcgc tgcttacatc gcagcttgaa gaggccatcg cccaactgga
    13861 ttgtgaagac ggcattgttt ttctgacgga tttgctgggc ggcacgccgt ttcgcgtagc
    13921 ttcaacgctg gcgatgcaaa aaccgggctg tgaagtgatt accggcacca atttgcaact
    13981 gctactggag atggtgctgg agcgcgaagg gttaagcggt gaagagttcc gtgtgcaggc
    14041 gctggagtgt ggacaccgtg ggctgacctg tctggtggac gagttaggcc gctgccatga
    14101 ggaatgtccg gtcgaggaag gaatatgaca cacgttctgc gcgccagaag gctgctgact
    14161 gaagagggat ggctcgatga ccatcagttg tgtattactg acggtgtcat cgcagcaatc
    14221 gaaccgattc cagtgggcgt gactgaacgc gatgcggaac tgctctgccc tgcttacatc
    14281 gacacccatg tacacggtgg tgcgggcgtt gatgtaatgg atgacgcgcc ggatgtgttc
    14341 gacaagctgg caatgcacaa ggcacgcgaa ggtgtcggca gttggttacc gaccaccgta
    14401 accgcgccgc ttaataccat tcatgcggcg ctgaaacgta ttgctcaacg ttgccaacgc
    14461 ggcggacctg gtgcgcaagt gctggggagt tatctcgaag gaccgtactt cacgccgcag
    14521 aataaaggcg cgcatccgcc ggagttgttt cgcgagcttg aaattgccga gctggatcag
    14581 ttgattgccg tttctcagca caccttacgt gtggtagcgc tggcaccgga aaaagaaggg
    14641 gcattgcagg ccatccgcca tcttaaacag caaaacgtaa gggtgatgct ggggcatagc
    14701 gcggcggcct ggcaacaaac ccgcgccgca tttgatgctg gtgccgacgg cctggtgcat
    14761 tgctataacg ggatgacagg tttacatcac cgcgaaccgg gaatggttgg cgcgggatta
    14821 acggacaagc gcgcctggct ggaactgata gccgatggtc atcatgtgca tccggcggca
    14881 atgtcgctgt gttgttgctg tgcgaaagag agaatcgtac tgatcaccga cgcgatgcag
    14941 gccgctggga tgccggatgg tcgctatacg ttatgtggtg aagaagtgca gatgcacggt
    15001 ggcgttgtcc gtaccgcgtc cggtgggctg gcgggcagta cgctatctgt cgatgcagca
    15061 gtgcgcaaca tggtcgagtt aacgggcgta acgcctgcgg aagccatcca tatggcgtcg
    15121 ctgcatccgg cgcgaatgct gggtgttgat ggtgttctgg gatcgcttaa accgggcaaa
    15181 cgcgccagcg tcgttgcgct ggatagcggg ctgcatgtgc aacaaatctg gattcagggt
    15241 caattagctt cgttttgatg gtttgctcca ttattgggcc ttcacttccc ccgtaaggcc
    15301 tttctttttc tttcgttttg atctgtgcag cggtgccgga tgcgacgcta acgcgtctga
    15361 tccgacctac ggtacgccgc atccggcaat cggtgaacat agcgacgtca ttacgcaatt
    15421 ctttaatctt cccttgtctt ttctttcaac gatcacagat ttcgttttat ttcccttccc
    15481 tccattgaac tttcggtttc ttttctatag attttaatca acgaaagaca tcaccaagtg
    15541 aaatgaaacg aaaggcaagt gaaagcaaca acgcccgacg tcaagttcat cagactaagg
    15601 attgagttat gccagaaaat tacacccctg ctgccgccgc aaccgataca tggactgaag
    15661 aagagatccg ccatcagcct cgcgcatgga tccgttcact caccaacatc gacgcgctac
    15721 gttccgcgct caataacttc cttgaaccgt tactgcgcaa agagaatctg cggatcatcc
    15781 tgaccggagc cggaacgtcg gcatttatcg gtgacatcat cgcgccgtgg ctcgccagcc
    15841 ataccggtaa aaacttcagc gccgtaccga ccaccgatct ggtcaccaat ccgatggact
    15901 acctgaatcc agctcatccg ctgctgttga tctccttcgg tcgatccggc aacagcccgg
    15961 aaagcgtcgc cgccgtggaa ctggcaaatc aatttgtacc cgagtgctat cacctgccga
    16021 tcacctgcaa cgaagcgggc gctctttacc aaaacgcgat caacagcgat aacgcgtttg
    16081 ccctgctgat gcccgcagaa acgcacgatc gcggctttgc gatgaccagc agcattacca
    16141 ccatgatggc cagctgcctc gcggttttcg cacctgagac gatcaacagc caaaccttcc
    16201 gcgatgtggc ggatcgttgc caggcgatcc tgacctcact gggtgatttc agcgaaggtg
    16261 tgtttggtta tacaccgtgg aaacggatcg tttatctcgg cagcggtggc ttacagggcg
    16321 cagcacgcga gtcggcgctg aaagtgctgg aactgactgc gggtaaactg gcggccttct
    16381 atgattcccc aaccggattc cgtcatggcc cgaaatcgct ggtcgataac gaaacgctgg
    16441 tggtggtatt tgtctcaagc cacccttaca cccgtcagta tgatcttgat ctgctggctg
    16501 aacttcgccg tgacaaccag gcaatgcgcg taatcgccat cgccgcggaa agcaccgacg
    16561 ttatcgccgc tggcccgcat atcatcctgc cgccatcccg tcactttatc gacgttgagc
    16621 aggcattttg cttcctgatg tacgcccaga cgtttgcact gatgcagtcg ctgcacatgg
    16681 gcaatacgcc ggatacccca tcagccagtg gcaccgttaa ccgcgtggtg caaggcgtaa
    16741 tcattcatcc gtggcaggca taagaggatc gcattatgag cattatctcc accaaatatc
    16801 tgttacagga cgcccaggcc aatgactacg cggtgcctgc ctttaacatt cataacgccg
    16861 agacgatcca ggcgatcctc gaagtgtgca gtgaaatgcg atcgccggtg atcctcgccg
    16921 gaacgccggg gacctttaaa catatcgcgc tggaagagat ctacgccctg tgtagcgcct
    16981 attccacaac ctacaacatg ccactggcgc tgcatctcga ccaccacgaa tcgctggatg
    17041 atattcgccg taaagtccac gcaggcgtgc gcagtgcgat gatcgacggc agccacttcc
    17101 cgtttgccga gaacgtaaag ctggtgaaat cggttgtcga cttctgccac tcccaggatt
    17161 gcagcgtgga agcagaactg ggccgcctgg gcggtgttga agatgacatg agcgttgacg
    17221 ccgaaagtgc attcctgacc gatccacaag aagctaaatg ctttgtcgaa ctgactggcg
    17281 tcgacagcct ggcggtagcg attggtacgg cgcacggctt atacagcaaa acgccgaaga
    17341 ttgatttcca gcggctggcg gaaattcgtg aagtggtgga tgttcctctg gtgctgcatg
    17401 gtgccagcga tgttccggat gaatttgtcc gtcgcactat tgaacttggc gtcacaaaag
    17461 tgaacgttgc cacagaatta aaaatagcct tcgctggcgc ggttaaagcc tggtttgcgg
    17521 aaaatccgca gggtaatgat cctcgttatt atatgcgcgt cggaatggat gcgatgaaag
    17581 aagttgtcag aaataaaatt aatgtctgtg gttcagcgaa tcgaatttca gcataatcat
    17641 tagtttttta tgatttatcc caatgtactt cccgattaat atcggggagt
//
LOCUS       DAKISA010000092        17337 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.092, whole genome shotgun sequence.
ACCESSION   DAKISA010000092 DAKISA010000000
VERSION     DAKISA010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17337
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.092"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            92..1132
                     /locus_tag="OVW45_003174"
     CDS             92..1132
                     /locus_tag="OVW45_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCV5527556.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLHTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(1164..2357)
                     /gene="lplT"
                     /locus_tag="OVW45_003175"
     CDS             complement(1164..2357)
                     /gene="lplT"
                     /locus_tag="OVW45_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCV5527557.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(2350..4509)
                     /gene="aas"
                     /locus_tag="OVW45_003176"
     CDS             complement(2350..4509)
                     /gene="aas"
                     /locus_tag="OVW45_003176"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCV5527558.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            5095..6126
                     /gene="galR"
                     /locus_tag="OVW45_003177"
     CDS             5095..6126
                     /gene="galR"
                     /locus_tag="OVW45_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCV5527559.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(6133..7395)
                     /gene="lysA"
                     /locus_tag="OVW45_003178"
     CDS             complement(6133..7395)
                     /gene="lysA"
                     /locus_tag="OVW45_003178"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCV5527560.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            7517..8452
                     /gene="lysR"
                     /locus_tag="OVW45_003179"
     CDS             7517..8452
                     /gene="lysR"
                     /locus_tag="OVW45_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCV5527561.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(8439..9131)
                     /gene="ygeA"
                     /locus_tag="OVW45_003180"
     CDS             complement(8439..9131)
                     /gene="ygeA"
                     /locus_tag="OVW45_003180"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCV5527562.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(9262..10680)
                     /gene="araE"
                     /locus_tag="OVW45_003181"
     CDS             complement(9262..10680)
                     /gene="araE"
                     /locus_tag="OVW45_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCV5527563.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(10995..11756)
                     /gene="kduD"
                     /locus_tag="OVW45_003182"
     CDS             complement(10995..11756)
                     /gene="kduD"
                     /locus_tag="OVW45_003182"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCV5527564.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQRNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(11821..12657)
                     /gene="kduI"
                     /locus_tag="OVW45_003183"
     CDS             complement(11821..12657)
                     /gene="kduI"
                     /locus_tag="OVW45_003183"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCV5527565.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(12944..14125)
                     /locus_tag="OVW45_003184"
     CDS             complement(12944..14125)
                     /locus_tag="OVW45_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCV5527566.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            14380..15609
                     /locus_tag="OVW45_003185"
     CDS             14380..15609
                     /locus_tag="OVW45_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCV5527567.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSACEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            16069..16701
                     /locus_tag="OVW45_003186"
     CDS             16069..16701
                     /locus_tag="OVW45_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCV5527568.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNTAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
     gene            17035..>17337
                     /locus_tag="OVW45_003187"
     CDS             17035..>17337
                     /locus_tag="OVW45_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCV5527569.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPREDS"
BASE COUNT         4593 a         4523 c         3989 g         4232 t
ORIGIN      
        1 ccggctggcc ttaagatttt tctctttccc ttttcccttc cctctgccat ttttatattc
       61 cttatgtcgt gattataaaa aggaaacggc tatgcaatat caccgtatac cccacagttc
      121 gctggaagtc agcacgctgg ggcttggcac gatgacgttt ggtgaacaga acagcgaagc
      181 cgacgcccac gcacaactcg actatgccgt cgctcagggc attaacctta tcgacgttgc
      241 cgaaatgtac ccagtacctc cgcgccccga aacgcaaggg ttaaccgaaa cctacgtcgg
      301 caactggctg gcgaaacatg gcagccgcga aaagttaatt atcgcctcca aagtgagcgg
      361 accgtcgcgc aataatgaca agggcatccg cccggatcag gcgctggatc ggaagaatat
      421 ccgcgaagcg ctgcatgaca gcctcaagcg cctacacact gattacctcg atctttatca
      481 ggtgcactgg ccgcagcgcc cgaccaactg cttcggcaaa ctcggttata gctggacgga
      541 ttctgcgcct gcggtttcac tgctggatac gctggacgca ctggcagagt accaacgcgc
      601 gggaaaaatt cgttatatcg gcgtgtcgaa cgaaactgca tttggcgtaa tgcgctacct
      661 gcatctggcg gacaaacacg atctgccgcg tattgtcacc attcagaacc cttacagtct
      721 gttaaaccgc agttttgaag taggtctggc agaagtaagc cagtatgaag gggtcgaact
      781 gctggcctat tcgtgcctgg gtttcggcac gctgaccggg aaatatctca atggtgcaaa
      841 acccgctggc gcacgtaata cgctctttag tcggttcacc cgctatagcg gtgagcaaac
      901 gcaaaaagcc gtcgcggcgt atgttgatat cgccagacgt catggcctgg accctgctca
      961 gatggcgctc gcgtttgtac gccgtcaacc gtttgttgcc agcactctgc tgggcgcaac
     1021 cacgatggat cagctgaaaa ctaacatcga aagtttgcat ctggagttaa gcgaagacgt
     1081 attagctgaa attgaagcgg tgcatcaggt ttatacttat ccggcaccat aagattaacg
     1141 ccggttaaaa ccggcgttaa atattaatga cggcgctgcc agatccacag cgccgttatt
     1201 gccagcgcaa acagcgcacc gaagccaatg ccaatgggca cgaccgggat gcctaccatt
     1261 accgccagcg agtaaatgcc cagcatcaac aacatggcgc tgttttcgcc aaggttttgt
     1321 actgcaatcg cattccccgc cccgacgctt tttttacccc gctcctgtag caacgcattg
     1381 agcggaacga caaaaaagcc ccccatcacg ccaatcagca tcaacaaggc ataggctggc
     1441 agcagctcgt gttgcaggga aaaaatcagt accaccacgc caatcaaaat cccggctggc
     1501 atacagcgtg acacggtttc cagcgtaact aacttcgctg ccgcacctgc gccaaccacg
     1561 ataccaatcg ctaccatcgc gttgagatag gtgggcgtag cgttatcggt aatgcccagc
     1621 gccaccggta cccacagcac caacaggaaa cgcagcgtga cacccgctcc ccagaataaa
     1681 ctggtgccca ccagcgaaaa acgcgtttca ccattgcgcc atagcgaggt gcaggcattc
     1741 aggaaactgc gggtcatgtt gatgagattc caggactgcc ccggacgcgc cgccgccagt
     1801 ttgggaatgt agatattggc aacgaccgca ccaccgtagg ccagtgcgca tgcggccagg
     1861 gcgacgagga catgccagtc agccagcaca ccaccggcta cggaaccgag caaaatcgcc
     1921 gctatggtag aagcttccat taaaccgtta gctttcacta acttactacc cgtggttaat
     1981 tcgccgagaa taccgtattt cgccggtgaa taggctgcag caccaacacc caccagcgta
     2041 tagccgagaa acggattgat accaaagcag atactggctg cgcccagcag cttcaggccg
     2101 ttggcaaaca tcatcacccg gcctttggcg aagctatccg ccacctgccc gacaaacggc
     2161 gcaaaaagaa tgtaagcacc tacaaacacc atttgcagga tgggctggct ccactccgga
     2221 tagaactgcg ctttcagtaa cgccagagtg gcaaacagta gggcattatc gccaaacgca
     2281 gagagaaact gcgccacgat aaccgctttc atccccttcg accacaacga agtgttagtg
     2341 tgcactgact cactcatcgt gttgttccgc ttcgtctacc cagcttttca acgtgacaaa
     2401 gtcaggtttg ccgctgccaa gtaatggcat ctgtttcaga tagcgaatat cgcgcggtac
     2461 agcaagctcc ggcacgccgt gctcgcgggc atactgttgc aacttatcgc gcgtcagttc
     2521 gttatctgtg gtgaaaagca ccagtgcctc gcctttgctg gcatcgctct taatcgcagt
     2581 ggcatggact ttatctggcg aaacaccaag tgccagttgt tccaccattt ccagcgacac
     2641 catttcgcct gcaattttgg caaagcgttt tgcgcggccc tgaatctgca caaagccctg
     2701 ctcgtcaaaa cgcacaatat cgccagtgtc ataccagccg cgctccattt cgccgcgaac
     2761 attctcggcg gtgggcactt ccagtacacc tggcttctcc acccgcagat agccgttcat
     2821 tatgttcggc cctttcagtt gcaggcgtcc gccctcttcg ataccaggga ccgacaacag
     2881 gcgcgcatcc attcctggta gaatacgccc taccgtaccg ggtttcgccg ccatcggtac
     2941 gttgatagaa acgacaggcg cgcattcggt cacgccgtag ccttcaagga tgcgcaggcc
     3001 aaatttatcc tgccaaagct gtttggtact ttcttgtaat ttttctgcgc ctgccaccac
     3061 atagcgtaga cgatagaagt catacgggtt ggcgaaacgc gcgtagtgac cgaggaaagt
     3121 cgaggtgccg aacaacacgg tgcaactgcg gtcatacacc aactccggca caatacggta
     3181 atgcagcggg cttggataaa gaaacacttc tgcacctgtg agcagtggcg taaacaggcc
     3241 taccgtcagc ccaaaggagt gaaacagcgg taacgccgac ataaagcgat cgttggtggt
     3301 gaagtcggca atcgttttaa tctgctcgac gttcgccaga atgcttttat ggctatggac
     3361 gacgcctttc ggatggcctt cagaaccgga ggtaaagagg atcagcgcct cttcttccgg
     3421 ctgctgtttt acctgtgcca gacgtggcat cagcagatgc gcgaagatcc atactttgtc
     3481 ggcagtggtg acatctgctt ttaaatcttc cagatagacc cagcgcacct gagtaagttg
     3541 ctccggcaga tgccagagtt tgcctttatc gagaaactgg cgggaagtga agatggtttt
     3601 gatttcagcc gccgtaatag cactggtcag cccttttacc ccggcagtgt agttcatcat
     3661 tgcgggaatg cggcgacggg cgatggcccc aaaaatcact gccgcactga tgcctgcatt
     3721 gggcagcatt aagccgatgc gttcgccttc aacactgtat ttttcaagga tgcgtccaac
     3781 aaacagcgtt ttcgtaagca atttgcgata ggagtctggg gtaaagttga cgtcttcgac
     3841 acatttcttc ccggctccga agcggtacat tgcactcagt aaagattcgt acagcgtttc
     3901 acgcgggcgc accgccattc gcgcttccat cattatttga tgcagcattt cgccagcgat
     3961 tttgcgacgg tcacgggcac gcggcgcatc cggcatctcc acctgcgttg gtggcaaaat
     4021 atgcagagta atttgcggga acaagcgacg tttaaccaga cctttcaggc ggctgaagtg
     4081 cgtaagttcc gccccttcaa tacgcacagg aataaccgtt gcaccagact tcgccgcgac
     4141 aaaacccgcg ccatcgtaga ttttcatcag cgagcctgtg gtggtgatgc gtccttcagg
     4201 gaaaatcacc actggtcggc cctgttccac cagacgtacc agatgtttaa tcgccatagg
     4261 ttgcgtcggg tcgagaggaa caaagtcgat aaatgatttc agccaacgca tataccactg
     4321 ttggcttatt gaggtgtaaa cggcaaacac tggacgcaca ggtaaaaaca gtccaagcaa
     4381 aatgccatca ataaaagaga cgtgattagg cgtaattaga acgcgctcgc ccttcagtgc
     4441 ctgggtgtca cccgtaacgc gaacgcgata caaaacacgg cacaaatttc gaaaaaagct
     4501 aaaaagcatt ccaactccct ttgctctgat tcagtaaaag cgaatggagg gagattacac
     4561 gagataaaga acgcgagcga cagtaaatta ggtgcgaaaa aaaacctgcg catccgcgca
     4621 ggttggtgca agagacaggg tacgaagagc gtaccgaata atctcaccaa tcaatacctc
     4681 tgggatcttg attgtggtct gcacgacgac tcttcgccag cgagaaaacg caaaggaatg
     4741 aagggaaatg caacgaggtg tgtaaattgt cggttactgt tacagattga tgaccggcaa
     4801 aaaaaaacct gcgcatctgc gcaggctggt gtaattcatg tgctcaaccc gaagttgact
     4861 tcacctatca atacctctgg gatcaccact ttagcaacct gaagccaaac gccaccagca
     4921 gtcaatcgca acagcatttc gcaaagtgta accaaaggtt tgcaatctct ttttttgtct
     4981 tacttatttg cgtttggctc acattcccac gatgaaaaca cgccgcccct tgaaccaacg
     5041 ggcgttttcc gtaacactga aacaatgtaa gcgtttaccc actaaggtat tttcatggcg
     5101 accataaagg atgtagcccg actggcaggc gtttcagtcg ccaccgtttc ccgcgtcatt
     5161 aataattcac ccaaagccag cgaagcgtcc cggctggctg tgcatagtgc aatggagtct
     5221 cttagctatc acccgaacgc caacgcccgt gcgctggcgc agcagaccac tgaaacggtc
     5281 ggtctggtcg ttggtgatgt ttccgatccg tttttcggtg caatggtgaa agcggtcgaa
     5341 caggtggctt atcacaccgg taatttttta ttgattggca atggttacca caacgaacaa
     5401 aaagagcgtc aggccattga gcaactgatc cgccatcgct gtgctgcgtt ggtcgtccat
     5461 gccaaaatga ttccggatgc cgatttagcc tcattaatga aacaaatgcc cggtatggtg
     5521 ctgatcaacc gtatcctgcc tggcttcgaa aaccgttgta ttgctctgga cgatcgttac
     5581 ggtgcctggc tggctacgcg tcatttaatt caacaaggtc atacccgcat tggttatctg
     5641 tgctctaacc actctatttc tgacgccgaa gatcgtctgc aagggtatta cgatgccctt
     5701 gctgaaagtg gtattgcggc caatgaccgg ctggtgacat ttggtgaacc agacgaaagc
     5761 ggcggcgaac aggcaatgac cgagcttttg ggacgaggaa gaaatttcac tgcggtagcc
     5821 tgttataacg attcaatggc ggcgggtgcg atgggcgttc tcaatgataa tggtattgat
     5881 gtaccgggtg agatttcgtt aattggcttt gatgatgtgc tggtgtcacg ctatgtgcgt
     5941 ccgcgcctga ccaccgtgcg ttacccaatc gtgacgatgg cgacccaggc tgccgaactg
     6001 gctttggcgc tggcggataa tcgccctctc ccggaaatca ctaatgtctt tagtccgacg
     6061 ctggtacgtc gtcattcagt gtcaactccg tcgctggagg caagtcatca tgcaaccagc
     6121 gactaaccgc agttaaagca attccagcgc cagtaattct tcgatggtct ggcgacggcg
     6181 aatcaaccgc gcctgaccat tatcaaacag aacttctggt aacagcggac ggctgttgta
     6241 gttggatgac attgatgcgc catatgcccc tgtatcatgc agtaccagat aatcacccgc
     6301 cttcacttcc ggcaaggcgc gggtttcaac atttccccct tcctgctggg taaagacatc
     6361 gcccgattca cataatggcc cggcgacgac ggtttccacc gttggcgcgt gttccagaga
     6421 acgaccatca gctgccaggg cactgatatg gtggtaacta ccgtacattg ccgggcgcat
     6481 cagatcgttg aacccggcat caaccagcac aaagtggcgg ctacccattt gtttgacgct
     6541 ccgcacctga gtaattaaca cgccagactg cgctaccaga aagcgacccg gttcaatttc
     6601 cagtttcaca gggtggccca aatggcgagc gatttgctca cgcgcggcat tccacagacc
     6661 ataataatgt tcggtatcaa ccgcctcttc accctgttga taaggaatag aaagcccacc
     6721 gcccgcagaa atagcctgta aatcctgacc gaattcgatg acctgacgta ccatagcacc
     6781 acacacctgt tccagatggg cataatcaac gccagaacca atgtgcatgt gaatgccgac
     6841 cagctgcaga tgatgacgtt gtatcacgtc cagtgcggcg ggcagatcgg tgtaccagat
     6901 accgtgcttg ctgttttcgc caccggtatt ggttttttgg ctatgtccgt gaccaaaccc
     6961 cgggttaacg cgcagccata cccgatgccc tggcgaaacc tggcccagtt ggtcgagcat
     7021 atcaacagaa cccgcattca ccggaatttg caattcactg acgcgttcaa gcgtcgcctg
     7081 atcgataaca tctgccgtaa aaacaatatc atcggggtgc gtttgcggat tgtaacccgc
     7141 cgccaacgca cgctctattt cgcctaacga gacggaatcc actttcacgc cctgctcacg
     7201 cattaatcgc aaaatatgaa tattggaaca ggctttctgt gcaaagcgca ccacatcaaa
     7261 ctgtttcagc gctgcaatct gccgacgaat aatttgcgca tcgtagaccc acaccgggca
     7321 gccaaattca gcgggcaaac gcagcagatt ttcggcggtg agatcggtat cggtgctgaa
     7381 cagtgaatgt ggcataacaa actccagatg agtgcttttt tatgattacg ccacatcata
     7441 aaaagaataa aaaatatcga tttatgtcga gtctatgcaa aaatgatata gattaccgga
     7501 ttgcgagaga gcgctaatgg ccgccgttaa cttacgtcat attgaaattt ttcatgcggt
     7561 aatgaccgcc ggaagcctga ctgaggcagc acacctgcta cacacctcac agccaaccgt
     7621 cagccgcgaa ctggcgcgct ttgagaaggt gatcgggcta aaattgtttg agcgcgtacg
     7681 tggacgatta catcctaccg tgcaaggact gcgtctgttt gaagaagtgc aacgatcctg
     7741 gtacggactg gatcgcattg tcagcgccgc agaaagtctg cgcgagtttc gccagggaga
     7801 actgtctatt gcctgcctgc cggtcttttc gcaatctttt ttaccgcagc tcctgcaacc
     7861 ctttctggca cgttatcccg atgtcagctt aaatatcgtg ccccaggaat caccgctact
     7921 tgaagagtgg ctctcggccc agcgtcatga tttaggactc actgagacac tccatacgcc
     7981 tgcgggaaca gaacgtaccg aattactctc tttagatgaa gtgtgtgtgt tacctccggg
     8041 tcatccgctg gcggtaaaaa aggtattaac gccggatgat tttcagggtg agaactacat
     8101 cagcctttcc cgtactgaca gctatcgcca gttgctggat cagctattta ctgaacatca
     8161 ggttaaacga cgcatgatcg tagaaaccca cagcgccgcg tcagtctgcg caatggtacg
     8221 ggcgggggta ggtgtttcgg tggttaaccc actcaccgca ctggattatg cggcaagcgg
     8281 tttagtggta cggcggttca gtattgcggt tccgttcacc gtcagcctga tccgccccct
     8341 gcaccgcccg tcatcagcgc tggttcaggc gtttagtgag catttacaag cggggttacc
     8401 gaaactggtc acttctcttg acgcgatttt gtcgtcagct acgacagcat aaaagcgaca
     8461 gcatcctcgg catggatcgc cgcagtatca aacacaggca aaacactgcg ctcttctgga
     8521 accaataaac caatttctgt gcagccaaaa atgacaccct gcgccccctg ttctgcaagg
     8581 cgagcaatca cttgcgcata ataagcgcgt gacgcttcgg taaattgccc cagacacagt
     8641 tcttcaaaaa taatctgatt aatcttcgcc cgttcatccg cttccggaat aagacaattg
     8701 atggaaaatt gttccgtcag ccgcccgcga taaaaatcct gttccatggt gtaacgcgta
     8761 cccagcagcg ccacacgagt cattccggcc ccggtaattg cacgtccggt ggcatccgca
     8821 atgtgtaaga aaggcagagt gcaacgtgac tcaatggcat ccgccacttt atgcatcgta
     8881 ttggtacata acacaatacc ttctgcgccc gcccgctgta agccaagcgc cgcctcagcc
     8941 agaatgtccc cggttttatc ccattccccg cgacgctggc actcttctat ttcatgaaaa
     9001 tcgacgctat gtagcagcac ttgcgcagag tgaagcccac caagccgctg tttaatgcct
     9061 tcatttatca gacgatagta aggaatggtg gattcccagc tcattcctcc cagcaaacca
     9121 attgttttca ttcctctctc tcctgatgtg tgttaccgca acaaggctga ataaggaacg
     9181 agacaaacgc ctcaacggcc aggtgcccaa tctctattaa cgaaaaaagg gccggatgta
     9241 cagcacatcc ggcccgtgaa atcagacgcc gatatttctc aacttctcgc ctgccatcag
     9301 tttgcgttcg atatgttcca gcgtgacatt tttggtttcc ggaatgagcc agaaagtgat
     9361 gcccacaaac gcaatgttca gcgcagtgta gagccagaac gtaccggcag caccaatgct
     9421 gtcgagcagc gtcaggaagg tcgcgccgat aatcatattc gacacccagt tcgtggtggt
     9481 cgaacaggta ataccgaaat cgcggcattt cagcggctga atttcagagc acaggatcca
     9541 caccactggc gcggcgctca tcgcataacc ggcaatacac atcatcgtca tgccaacaga
     9601 gagccaggac aagccactgg aagccgtacc gttatcaaac tgcatcaggc aatagcccag
     9661 caccagagtg cctaacgcca tcacgctgaa accaattttc agagccggtt tacgccctgc
     9721 tttatctacc gtaaacaccg caataaaggt ggcgaacata aaggtcagcc ctacgaccag
     9781 agtcgcaatc atctgttgtt ctgtggtcgt aaagcccgcc attttgaaga tacgcggcgc
     9841 gtagtacatg atgatgttca taccggtaaa ctgctgcatc gcctgcaaca acataccgag
     9901 aaacacagca cgacggacgt tacggttgat cttaaacagt gcccaaccgc cctgttttaa
     9961 cttcaggctt tcacgaattt cgttgagttc ttctcgcgct ttttccgacg tatcccgcag
    10021 catacgcaac acttcttccg cctcaatatg acgccccttt tccgccagcc agcgcgggct
    10081 atttggcagg aagaccacca gaataatcag caggactgct ggtaaagcaa gaacccccaa
    10141 cattgcgcgc cagttaccgc tataactgaa cgctgtatcg gataaaaacg ccagcacgat
    10201 gccgagtgtg accatcaact ggtacatact gatcatctta ccgcgaacgt tttcacttgc
    10261 catttcagaa agatacagag gagcggtgta agacgcgatc ccgacagcaa tgcccagcac
    10321 cacccgagcg gcgattaaca tctctacgct ggtcgcaaaa gcggacccta tagaaccgag
    10381 tacaaacagg atggcccccg ccatcaggct gtatttacgc cccaggcgga acgacagcca
    10441 accattaaac agcgcaccaa ttgctgcgcc gagcatcatg ctactaacca cccattcctg
    10501 caaacgactg gtcagcacaa agtgatcggt aatgaacggc aacgctccgg cgattacgcc
    10561 gatatcaaga ccaaataaca atcctgcgac cgcagcagct accgaaacaa acatattcat
    10621 acgccgcgta tcccgcaaag aacgtggcgt taaagcagat tccgtattga tagtaaccat
    10681 tttttcctgc cagcagagag taagacatag tgaaaaaata cgtgaacaac tcacacaggt
    10741 gtcaggtcgg aaacagcata aatatggatt aaattgctgc gacatgtcgt tatgtgatgg
    10801 atattccaat tttcaaatta agttgaatta ttgagattat tattaaccac ctaattttac
    10861 agcagataaa attcataaag ttcattaatt gataattgat atggattatt tcataaccat
    10921 gatatgaatt atgatgacct acaggtataa aaaaccctgc catgaggcag ggtcataaaa
    10981 gtaagaggaa tgaattaacg agccagccaa ccgccatcta cggcaatggt ataaccattc
    11041 acataatctg aagcgctgga ggcaaggaac actatcggcc ccatcaggtc actcggcagt
    11101 ccccaacgac cagctggaat gcggtcgaga atttccgcgc tacgttgttc atctgcccgt
    11161 agttgttgag tattgttggt cgccatgtaa cccggagcta tcgcattaac attaatgttg
    11221 tgtttagccc attcgttcgc catcaatcgc gtcacgccca tcacgccgct ttttgatgcg
    11281 gtataagaag gcacacggat cccgccctgg aaggagagca ttgacgcgat attgataatc
    11341 ttgccgccat tgcgttgcgc gataaagtgt ttcgccgctg cctgagacat gaagaatacg
    11401 ctcttgatat tcaggttcat gacatcgtcc cagtcttttt cgctgaactc gagagcatct
    11461 tcgcggcgaa tcaatccggc gttattcacc aggatatcaa tatgaccaaa ctcagctacc
    11521 gcgcgatcca gcagtgctgg aatgccatca atctttcgca gatcggcggt caggcttaaa
    11581 aaacgacgcc ccagcgccgt gacctgcttg atggtttcag tcggttcaac gatgttaatg
    11641 ccaacaatgt cacagcccgc ttgcgccagc cccaacgcca tcccctggcc cagtccagta
    11701 tcacaaccag tgacgaccgc aactttacct tcgagagaaa atgcacttaa aatcataaca
    11761 ataccttatt cgtttatgcc gacaactagc gcaaatcttt aacggccact atgccgacaa
    11821 ctagcgcaaa tctttaacgg ccacatggtc catatcatca aagacctggt tttcaccaac
    11881 catgccccag ataaaggtat aagctttggt tccgacgccg gaatggatcg accagctggg
    11941 ggagatcacc gcctgctcgt tatgcatcac aatgtgacgc gtttcttgcg gctgccccat
    12001 catgtggaaa acgcaggcgt catcatccat attgaaatag aaataaactt ccatccggcg
    12061 ctcgtgagtg tgacacggca tggtgttcca caagttacct ggagccagct ccgtcagccc
    12121 catactcaat tggcaggttt ccagcacatc cgggacaaaa tatttgttaa ttgtgcgacg
    12181 gttactggtg aggttatcgc ctaacgtgac tggagatact tcgtccggtg tgactttttt
    12241 ggtgggatac gtcgtatgcg cgggtgcgca attgtaataa aactttgccg gagtggcggt
    12301 atcaatactg gcaaagacaa cttcttttgc acctttacca acatacaggg cgtcgcggtg
    12361 accgatttca tagcattggc catcgacagt aatcgtaccg gcaccgccaa tattgataac
    12421 acctaactcg cgacgttcaa ggaaatagct tacgccgagt tgtttaccaa cttccccgcc
    12481 aacggaaacc gttttagtta tcggcataat gccgccaaca ataattcggt caatgtggct
    12541 gtaaaccatg gtgtactcat cggcgacaaa taccttttca accaaaaatt cattgcgcag
    12601 cccttgggta tccagcgttt ttgcgtgcgc actgtggatg ctctgtctta cgtccacatc
    12661 aacctccgat aatcagtgaa gcgtgaaaat agaaaacgat agtgaaacac tgtttcgttt
    12721 tctctataaa gggatgatat gcccatgggc tagggttttc aattaaaaat aaaacaatgt
    12781 ttcattttta agttaaggat taaaaaaagt gcagtgtcga tcacgaatat gtcatgagaa
    12841 tggaaaaatg agggagccca gaaaatgtat gtcgacttgc cagccatcat tattcaacag
    12901 attaatgacg gcagatattt atcggtaagg aggaaatgca gagctactcg tcacgttcaa
    12961 tggtcaatgc cacgccctgg cccccgccga tacaaagcgt tgccagccct ttgcgggcat
    13021 tacgtttcac catttcatga accagagaaa ccaggattcg gcaaccggaa gcgcctatcg
    13081 ggtgaccgag tgcgatcgcg ccaccattga cattgacccg acgctcatcc cactcaagca
    13141 tcttgccaac cgaaagcgcc tgtgcagcaa acgcttcatt agcctcgata agatcgacat
    13201 cagccaactg ccagcctaca cgctccaggc aacggcgggt cgcatacacc ggcgcaattc
    13261 ccatcaatgc cggatctaca ccaacgctgg caaatgcgcg gatgcgggcc agcacgggta
    13321 aattcaacgc tcgtgctttg gcttcgctca tcatcattac cgcagctgcg ccatcgttta
    13381 tggatgatgc attccccgcg gtcacagaac cgagactatc aaatgaagga tttaaacggg
    13441 ctaaaccttc tgcactggcg tcagtgcgtg gctgttcatc ggtatcaaca accaacgtct
    13501 gaccgttact ttggatcatt accgggacga tctcatcttt aaatcgtccg gcgtcaatcg
    13561 ccgctcgcgc tttttgttgc gagctaagtg cgtaagcatc ctgcaactga cggctgatgc
    13621 catattcgcg agccagattt tcggcggtga caccaatatg ataatcattg aaggcatccc
    13681 acaacccatc atgcaccaga ctgtcaacca actggctatt gccaagctgt gcaccggtgc
    13741 ggctatcagt cagaacatgt ggtgcgcggc tcatgttttc ctggccaccg gcgatgacaa
    13801 tatcagcctc gccacactgt atcgcctgag tagccagatg cagtgcttta agtccggaac
    13861 cgcaaacgtc attaatcgtg attgcagaaa cgctattagg aagaccacct ttaatagccg
    13921 attgccttgc cggattctgc cctgcccctg cagtcaacac ctgaccaaga attacttcat
    13981 ccaccgcata tgcaggaacg ccggtacgtt ctattaacgc tttcacgacc agactaccaa
    14041 gttccacggc ggaatgaccc gctaacgcac cacgaaagca gccgataggt gtccgtaacg
    14101 ccccgacaat cacaacgtct ttcatcacaa cctcgcacca attaacagtg caatagtaaa
    14161 tgattgttaa ttacagttat cttaattgtt taaaaaaagt gatttttatc acaaaggaaa
    14221 tatgcctgag cagtagtcag agtcataact ggcacgtaag gtttgcaacc actaacccac
    14281 caatagatgg gtagataagg cgttaatctc ccatacttaa cctggtttat ggtaaattgc
    14341 cctccatttt gtttaatttg tagatgatac gttcagataa tgtctaatat ttggtctaaa
    14401 gaagaaactc tgtggagttt cgcgctctac ggcacagccg ttggtgcagg cacgctcttc
    14461 cttcctattc agttaggttc ggcaggggct gtggtcctgt ttattactgc tctggtcgcc
    14521 tggcctttaa catattggcc acataaagcc ttatgccagt tcatcctctc atcaaaaaca
    14581 tcagcatgtg aagggataac gggcgcggta acacactact atggcaagaa gattggtaat
    14641 ctgattacca cgctgtactt catcgccttt tttgtcgtcg tgttgatata tgcagtggca
    14701 attaccaact cacttacgga acagctggca aagcatatgg ttattgatct tcgcatccgt
    14761 atgttggtga gtctgggtgt tgtattaatt ctgaatctca tttttctgat gggacgtcat
    14821 gccactattc gggtaatggg atttttggta ttcccattga ttgcctattt cttatttctt
    14881 tccatttacc ttgtcggtag ttggcaacct gatctattaa cgacccaggt agagttcaat
    14941 cagaataccc ttcaccagat atggatatcg attcccgtga tggttttcgc ctttagccat
    15001 acgcccatta tttctacgtt tgccatagac agacgtgaaa aatatggcga acacgctatg
    15061 gataaatgca aaaaaattat gaaagtcgct tatctcatca tctgcataag tgtactgttc
    15121 tttgtcttta gctgcctgct ttctattcca ccttcgtata ttgaagcggc taaagaagaa
    15181 ggggtcacca ttttatcggc gctttctatg ctgccgaacg ccccagcatg gttgtcaatt
    15241 tccgggatta ttgtcgcagt agttgcgatg tcgaaatcat tcctgggtac gtactttggc
    15301 gttattgaag gtgccacgga ggtcgtcaaa acaacactac agcaggttgg tgtaaagaaa
    15361 agtcgtgcat ttaaccgcgc actatcaatt atgttggtat cgctgattac cttcattgtt
    15421 tgttgcatta acccgaacgc gatttcgatg atttatgcga tcagcggccc gctcattgcc
    15481 atgatacttt tcatcatgcc tacgttgtca acgtatctga tcccggcgct taaaccctgg
    15541 cgctccatcg gaaatctgat tacgctgatc gtgggtatcc tgtgcgtatc ggtaatgttc
    15601 tttagctaat atccgttgtg gatggttcaa aattgctaca attttgaacc attccatttt
    15661 tcataatatt catttgttat tattccgccc tatcacttct ctcgtcatat cctagaatta
    15721 tcagttagtt aaacacacaa agagatctga acatcttaaa taagactatt taagatgcat
    15781 aacttagatt cgcaagatat cttctggtca ttcaaaaaca atttgcgata tagatattct
    15841 cataccatga gatagttatc tggaccacgt tcgaccatgc aagattaaat cagtgaaaca
    15901 taatcatatt taataacgag aaatgcattt ttaaatgtct taatcagaat tatagcaaat
    15961 acagattaat cgcaattttt cccagcaaaa ttacaaaatc aagatgtatt gacatcatca
    16021 atcaccaatg aaattatgca attatatacg gatagggagg ttcttaacat ggggctttgt
    16081 agtcgttata aaagtcttac atgcaatagt tgctcaatgc attgccaaat aatgccagaa
    16141 gagtcaccgc gtttgcagta ttgcgcaaac tcgtgttttt gtatgtggcc cgaagaaagt
    16201 tcatatttta atcgtggtgt ggtggaaggt atcttaacaa aaaaccacaa tgcgagatta
    16261 agcggatata tttttgtgga tttttcagta agttttttac gtctattcct tgaaaaagac
    16321 tggattgact atcttgcaag tactgacatg gggatagttt tagtcagcga ccgcaatatg
    16381 caatcgttag ctaactactg gcgaaaacac aacaccgcaa tatctgctgt catatataat
    16441 gatgatggtc ttgatgttgc caatgaaaag atcagacaac tatttattgg tcgttattta
    16501 tcatttacca gaggtaacac attaactcag atggaattca ccataatggg gtatatggtt
    16561 tctggttata atccatatca gattgctgaa gtgctggata tggatatccg tagcatctac
    16621 gcgtacaagc aacgaatcga aaagagaatg ggtggtaaaa taaacgaatt atttattcgt
    16681 tcacattcgg tccaacattg atactcaacg accagccaga atcatactct gttcatacgg
    16741 gcatctattg aaatgcccgt attttataaa taagcagtaa ccccaagagt aaacaacata
    16801 atatttaaaa ccatcacacg aaaaattcag aagcattacg aaatatgaat ttacaaacaa
    16861 tagtggcata aatgttaacc atgttaattt acgtaaagtt ttacgttgca acattaaagc
    16921 ctcatttcaa tcatcatgat aaatataaaa ttaatatata tttatgccgt aaataccaat
    16981 atacttagca aactatgtga tctccatttc gattgattta gtgtttattg acgtatgtac
    17041 tggattatta acgataatat cgagttctgg cctgagcacc gaaaattaat atcggtacat
    17101 aacgccgatc ttaacgtcgt tctgacaacg ccagccagtc gatgtttatc acttctactt
    17161 gaagcttttc ctgatgtggt tgcacaacaa gattttttca ccagagtctg ggaagaagaa
    17221 ggtatgcgtg tgcctactaa cacgttatat cagaacatat ccattatcag acgcggattt
    17281 cgcgctgttg gtgatactac ccactcgcta attgcaaccg tgccgagaga ggattca
//
LOCUS       DAKISA010000093        17202 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.093, whole genome shotgun sequence.
ACCESSION   DAKISA010000093 DAKISA010000000
VERSION     DAKISA010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17202
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.093"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..369
                     /locus_tag="OVW45_003188"
     CDS             <1..369
                     /locus_tag="OVW45_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCV5527570.1"
                     /translation="AMMYAETRNMTPYGNVGIANKTQNFEAVAQYQFDFGLRPSLAYV
                     YSKGKDLGGNDYNNNGHQEYVDQDLVNYVEIGATYYFNKNFSTYVDYKINLLDKDDDF
                     YDNNGIATDDVVGVGLVYQF"
     gene            complement(509..704)
                     /locus_tag="OVW45_003189"
                     /pseudo
     CDS             complement(509..704)
                     /locus_tag="OVW45_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353128.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            960..1811
                     /gene="hchA"
                     /locus_tag="OVW45_003190"
     CDS             960..1811
                     /gene="hchA"
                     /locus_tag="OVW45_003190"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCV5527571.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAD"
     gene            complement(1919..3277)
                     /gene="hprS"
                     /locus_tag="OVW45_003191"
     CDS             complement(1919..3277)
                     /gene="hprS"
                     /locus_tag="OVW45_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCV5527572.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRIMLPQ
                     RN"
     gene            complement(3277..3948)
                     /gene="hprR"
                     /locus_tag="OVW45_003192"
     CDS             complement(3277..3948)
                     /gene="hprR"
                     /locus_tag="OVW45_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCV5527573.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            4081..4494
                     /gene="hiuH"
                     /locus_tag="OVW45_003193"
     CDS             4081..4494
                     /gene="hiuH"
                     /locus_tag="OVW45_003193"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCV5527574.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            4603..5607
                     /gene="msrP"
                     /locus_tag="OVW45_003194"
     CDS             4603..5607
                     /gene="msrP"
                     /locus_tag="OVW45_003194"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HCV5527575.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPTAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            5608..6243
                     /gene="msrQ"
                     /locus_tag="OVW45_003195"
     CDS             5608..6243
                     /gene="msrQ"
                     /locus_tag="OVW45_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCV5527576.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFKRLRKQVHNKLSV"
     gene            6500..7150
                     /gene="zinT"
                     /locus_tag="OVW45_003196"
     CDS             6500..7150
                     /gene="zinT"
                     /locus_tag="OVW45_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCV5527577.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKIKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQFLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            7493..8023
                     /locus_tag="OVW45_003197"
                     /pseudo
     CDS             7493..8023
                     /locus_tag="OVW45_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(8593..8682)
                     /locus_tag="OVW45_003198"
     tRNA            complement(8593..8682)
                     /locus_tag="OVW45_003198"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8646..8648),aa:Ser,seq:cga)
     gene            8776..9573
                     /gene="mtfA"
                     /locus_tag="OVW45_003199"
     CDS             8776..9573
                     /gene="mtfA"
                     /locus_tag="OVW45_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCV5527578.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            9674..9749
                     /locus_tag="OVW45_003200"
     tRNA            9674..9749
                     /locus_tag="OVW45_003200"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9707..9709,aa:Asn,seq:gtt)
     gene            10063..16816
                     /locus_tag="OVW45_003201"
                     /pseudo
     CDS             10063..16816
                     /locus_tag="OVW45_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            complement(17078..>17202)
                     /locus_tag="OVW45_003202"
     CDS             complement(17078..>17202)
                     /locus_tag="OVW45_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase family
                     protein"
                     /protein_id="HCV5527579.1"
                     /translation="YKTRWPAMADVSESVVVGQKIDNISLDEFAKALKSVLARI"
BASE COUNT         4869 a         4086 c         3822 g         4425 t
ORIGIN      
        1 gcaatgatgt atgctgaaac ccgcaatatg acaccatatg gtaatgtggg tatcgcaaat
       61 aaaacccaga actttgaagc tgtagcacag tatcagttcg attttggtct gcgcccatct
      121 cttgcatatg tttattcaaa aggcaaagac ctcggcggta acgactataa caacaatggt
      181 catcaagagt atgttgatca ggatctggtc aactatgttg aaattggagc gacctactat
      241 ttcaataaga acttctcaac atatgttgac tataaaataa acttgttgga taaagatgac
      301 gatttctatg acaacaatgg catcgcaaca gatgacgttg ttggtgtggg cttagtctac
      361 cagttctaat cagccaacgt gctgcggcgt gttactgtaa aactaaacac tagcctgagt
      421 tcgtccctca tcgaattcag gcttttttat tggcatttca tctccatctg cggcacacgc
      481 aattgccaat gcatatcgat acagcacctt accctgcccc tcctggcaca ataacatcaa
      541 ccagcgactc ttcaaaccaa cacccaaatg catttcttta tgaattaatc cgtaacaaac
      601 acgcttccca tatagaaata tttgcaaaaa tagtgcatga aataatcttt tctctaccaa
      661 tccgcgatat aaatattcat cagtaccaca atatttcaca gcactaaatc tctccccgcc
      721 gccccgtacc tttgataatg gtctaaaatc attgaggcca cttgcgacga cgttgctcaa
      781 taaggaaaat ttggcgtgaa taatattcaa caccagtgca gcatcatcat tacatactta
      841 cacccgccga ctaccgcttt cgatatattg actcaatttt ccagactggt taaaaatagc
      901 gaccagctca gtcgcaaata tagtgactac cctaactaag caacaataag gaatacacta
      961 tgactgttca aacaagtaaa aatccgcagg tcgatattgc tgaagataat gcattcttcc
     1021 cttcagaata ttcgcttagc caatatacca gtcccgtctc tgatcttgat ggcgtggact
     1081 atccaaaacc gtatcgcggt aagcataaaa ttctggtgat cgccgcggac gaacgttatt
     1141 tgccaaccga taacggaaaa ctgttctcga ccggtaacca tccgattgaa acgttgctgc
     1201 cgttgtatca tctccatgct gcaggtttcg aattcgaagt ggcgaccatt tccggtctga
     1261 tgaccaagtt tgaatactgg gctatgccgc acaaagatga aaaagtaatg ccattctttg
     1321 agcagcataa atcgttgttc cgcaatccga agaaactcgc ggatgttgtc gccagcctca
     1381 acgctgatag cgaatatgca gcaatctttg ttcctggtgg tcatggcgca cttattggtt
     1441 taccagaaag ccaggacgta gctgctgctt tgcagtgggc aatcaaaaat gaccgttttg
     1501 ttatctccct ttgccacggc ccggcggctt ttctggcgct tcgccacggc gataacccac
     1561 tgaatggtta ttccatttgc gcattcccag acgccgcaga caaacaaacg ccagagatag
     1621 gctatatgcc gggtcatctc acctggtact tcggcgaaga gctgaagaaa atgggcatga
     1681 acattattaa tgacgacatc accgggcgag tacataagga ccgtaaagtt ctcaccggcg
     1741 acagtccttt cgcagcgaat gcgttgggta aactggcggc gcaggaaatg ctggcagctt
     1801 atgcggatta atccctgtta cgtttgaatg taaacgcata atcaatcgca gccacgttaa
     1861 tactcacgac gtggctgcgt tttttcatgg ttgatttatg gcgtactgaa gccctatgtt
     1921 aatttctttg cggtaacata atccggaaca cattatgctt attgagatag tgatacgtag
     1981 cacttccccc atgtaattcg gcaatcgctt tgactaaaga aagaccaagt ccctgaccta
     2041 cggaatgacg cgaattatct ccccgccaaa atctacggaa gagtttttca ggctcatgaa
     2101 ttttcgttcc aggactggcg acatcaatat taagatagcc gttggtatca agaaaactgg
     2161 ttatatgaat acgcgatttt tctggtgaat atctaatggc attaacaata agattcgata
     2221 acattcgttg cagtaaaatt ttatccgcaa agatttgctg attgcactcg accttaaagc
     2281 aaatctcttt ctcgtctgaa aggtattcaa gataatccaa caaattttcg acttccttat
     2341 tgagagaaag cgagtccagt ttcaccaaaa cattgttttt atctgcccgg gcaagaaaca
     2401 gtatgttctc tgttaaccgc gaaatatttt ccagctcttc aatgttaccg gcaattgttt
     2461 tttgatattc agcgatactt ctggtttgac tgagcgtaac ctgattctga cccagtaatg
     2521 cattaatggg cgttctaagt tcatgagcga gatcgtcagc aaattgactt agacgctcaa
     2581 aatctttgac taaagcatga tgcattttat tcaacgcctg cccaagaggt tttagttctc
     2641 tcggtaatgc gctaacctca acaggctccc ggctatcgtt ataattcagc gcttccgtta
     2701 caccactcaa ctttttgatc tctcgtaatc ccgttctgat taacagcgga cttaatactg
     2761 agcaaagtac aatggcgaca atgcaaatta taatgctatt aattttatac tgttcaagca
     2821 tgttatgtct ggctgaagcc aatttagcca cagtaaccgt taatgatggc gaaacttcat
     2881 caatattaat tcgtaaagca tcaatctcag tatcattaat aatgcttctg taaatgccag
     2941 ctgcgctgat tgtctcacta gcaggtatgt tatttaacat gtcatcactg acatttgtcc
     3001 ggttaacaat tttattgatg ccatcaccat gaatgatcaa gatatcctga ctaacatcca
     3061 tcatccggtt aaagtataca ggtaacgtat ctggatttac cccatcaatt aacaactgct
     3121 tgatctgtgc tgtccggtta atgagtgttg tatcatcgcg ccatttcaac tcacttgcca
     3181 ggccattata gagagtccag acaattccag cgccagcaac agacagcagc aatataaaaa
     3241 gcaaggttaa acggacggtt atagataatc ttttcattat ttttttaccg ctacgaatga
     3301 atagcccatc ccccggattg tggcgattag cttttcagga aaaggatcat caacttttgc
     3361 gcggagcctg cgaatggcga catccaccgt attggtatca ctatcaaagt tgattcccca
     3421 aatttcactc gcaataaccg ttctgggtat aatttcgcca gctctggagg ccagtagcca
     3481 aagtaactga aactccttgc gcgtcagtgt aatactgata ttgtccctgc tcacactttg
     3541 acttacagag tccattctta agccactgat ttctaatgtt gaattcaaag cgtgatgttg
     3601 ccttaattgt gcccgaaccc ttgccagcaa ctcagaaaat gaaaaaggtt ttaccagata
     3661 atcatttgct ccactgtcca gccctctgac tctgtcatcg acagaatccc ttgcagtaag
     3721 gcaaataaca ggggtttgct ttgctgttct taacgtttgt aagatctgcc agccatccat
     3781 acccggaagc ataatatcca gaatgatcaa tgcataatca tccttcagcg caagataaag
     3841 cccatctctg ccatcagaaa cggcatcaat gacataaccc gcttcggaaa gcccctgcgt
     3901 tacccactcc tgggtccttt gattatcttc aataagtaaa atcttcatga aaataatatg
     3961 ccataaatca tatgttatag aaacagcctg gttcattaca aaattgtaat gctgctgtaa
     4021 ggttaccctg gccgcttttt cgctatcctc aaaactcatt cacatgacaa ggatataaac
     4081 atgttaaagc gttatttagt actctccgta gtaacggcag cattttcatt accttctttg
     4141 gtttatgccg cacaacaaaa cattcttagc gtgcacattt tgaaccagca aaccggaaaa
     4201 cccgctgccg acgtgacagt cactcttgaa aagaaagcgg acaacggctg gttacaactt
     4261 aataccgcca aaacagataa ggatggacga attaaggcac tgtggcccga gcaaactgca
     4321 actacgggcg attaccgtgt cgtatttaaa accggggact atttcaagaa acaaaatctt
     4381 gaaagtttct tccctgagat ccccgttgaa tttcatatta ataaagtgaa cgagcattat
     4441 catgtgcctt tattacttag ccaatatggg tattcaacct atcgtggcag ttaatgcata
     4501 aatatgtaaa agcagatctc tgctactgtt agggaggaca ctccctgaca gattaacagt
     4561 aaacggctct tgctggctaa cgacaaaaaa gtgtgatggc ttatgaaaaa gaatcaattt
     4621 ttaaaagaat cagatgttac ggccgagtcg gtattcttta tgaagcgtcg acaggtgtta
     4681 aaagcactgg gcatcagcgc agctgcactt tctttgcctc acgctgcgca tgccgatctg
     4741 cttagctggt ttaaagggaa cgatcgcccg cccgcccccg ccggaaaacc gctggagttc
     4801 agcaagccta ccgcctggca aaataatctg ccactgacgc cagtagataa agtctccggt
     4861 tataacaact tctatgaatt cgggctggat aaagccgatc ccgccgctaa tgctggtagc
     4921 ctgaaaaccg atccatggac actgaaaatc agcggcgaag tggcaaaacc attgaccctc
     4981 gatcacgatg atttaacccg tcgcttcccg ctggaagagc gtatttatcg tatgcgctgc
     5041 gtggaggcat ggtcgatggt ggtgccgtgg attggttttc cgctgcacaa attgctggcg
     5101 cttgccgaac ccaccagcaa tgcgaagtat gtcgctttcg aaacaattta tgcaccggaa
     5161 caaatgccag gccagcagga ccgctttatc ggcggcgggc tgaaatatcc ttatgtcgaa
     5221 ggattgcgtc tcgacgaagc aatgcatccg ctcacactga tgaccgtggg tgtttatggc
     5281 aaggcgttac cgccacaaaa tggcgcgccg gtacgactga ttgtgccgtg gaaatatggc
     5341 tttaaaggga ttaaatcgat agtcagtatt aagctgaccc gcgagcgtcc gccaaccacc
     5401 tggaatctgg cagcgcctga cgaatacggt ttttacgcca acgttaatcc gcatgttgat
     5461 cacccgcgct ggtcacaggc taccgaacga tttattggtt caggcggcat cctcgatgtt
     5521 cagcgccagc caacgctact gtttaatggt tacgccgacc aggtggcatc gctgtatcgt
     5581 ggcctggatt tgcgggagaa tttctgaatg cgtctgacag caaaacaggt gacatggctg
     5641 aaagttagcc tgcatcttgc cggattgttg ccgtttctct ggctggtctg ggcgatcaat
     5701 cacggtggac tgggtgccga tccggtgaaa gatattcagc attttaccgg tcgcactgca
     5761 ctgaaatttt tgctggcgac cttgttaatc acacctctgg cacgctacgc aaaacagccg
     5821 ttattgatac gcactcgccg cctgttaggc ttatggtgct ttgcctgggc gacactgcat
     5881 ttaaccagtt acgcattgct ggagctgggc gtgaataatc tggcgttatt aggcaaggag
     5941 ttaattaccc ggccttattt aacgttagga attatcagct gggttatttt gcttgcttta
     6001 gcgtttactt caacccaggc gatgcagcga aaactcggca agcactggca acagttgcat
     6061 aatttcgtct atcttgtcgc gatcctggcc ccaatacatt atctgtggtc agtgaagatt
     6121 atttcaccgc agccgctcat ctacgctggg ctggctgtac tgcttttagc cttacggtat
     6181 aagaagttgc gttccctttt taagcggtta cgcaagcagg ttcacaacaa attatctgta
     6241 taacttcata ttgccgacaa agtacgcgtt atccctgccg gatgtggcgc gagcgccctg
     6301 tccggcctgc gcagatcaca tcaggttatg tttcgtttgt catcaacctc aaattatcaa
     6361 cataattcat tttatttcaa taagatagat aagtacacct gagaaaacta tgctctcatc
     6421 tcctaagagt tgttgcattt tgccatatgt tacaatataa cattacacat catatacaat
     6481 gactctggag gaaactgttt tggcaattcg tcttcacaaa ctggctgttg ctttaggtgt
     6541 ctttattgtt agcgctcctg ccttttcgca tggtcatcac tcacacggca aacccttaac
     6601 agaggtcgaa caaaaagctg ctaatggtgt ttttgatgat gccaatgtac aaaaccgaac
     6661 gctcagtgac tgggatggag tctggcaatc cgtttatcct ttactgcaaa gtggcaaact
     6721 tgaccccgtc tttcagaaga aagcggatgc agataaaatt aaaacatttg ctgaaattaa
     6781 agattattat cacaaaggtt atgcaacaga tatcgagatg attggcattg aggacggcat
     6841 tgttgaattc catagaaata atgaaacaac atcctgtaaa tatgattacg atggatacaa
     6901 aatactcacc tataaatcag gcaagaaagg cgttcgttac ttattcgaat gtaaagatcc
     6961 tgaaagcaaa gcccctaaat atatacaatt tagcgatcat ataattgcac cacgaaaatc
     7021 atctcatttt cacattttta tgggtaatga ttcacagcaa tttttattaa atgaaatgga
     7081 aaactggcca acgtattatc catatcagtt gagtagcgaa gaagtggtcg aggaaatgat
     7141 gtctcattga gtattctcat gataacgcct cgatgccgct ttagtaagtc atcataactg
     7201 ccactggtcc atccacaaac gccactgaac gcaagctagc tacagacacg ctcatcacta
     7261 tgacgtgtct gtatattaat aagctaaccc gcattgagtt aaccaataac ggattccata
     7321 cacaatacgg ccaacagaga aagatacctg tgctcacgcc attgcttata ttggctggtt
     7381 acaatgtgca ctatcaattt tttaaataac aaattcttaa aaccacatct gtataaggaa
     7441 tttttaaggt tcgtgggata gcttgactgt aaaaattaca ggagctacaa aaatgaaccg
     7501 attctcaaaa actcaaattt atttacattg gataacgctg cttttcgttg caataaccta
     7561 tgccgcgatg gaactccgtg gctggtttcc taaaggtagt agtacttatc tgctgatgcg
     7621 agaaacacat tacaatgcgg gtatattcgt ttgggtgtta atgttttcac gcctgattat
     7681 aaaacaccgt tatagtgatc cttctattgt accaccgcca cctgcctggc aaatgaaagc
     7741 ggcttcgcta atgcacatca tgctttatat aaccttcctt gcacttcctc tgctggagat
     7801 tgctttgatg gcttacagtg gaaaatcgtg gagtttcctt ggtttcaatg tgtctccctt
     7861 tgttacccca aacagcgaaa ttaaagcact gataaaaaat attcacgaaa cctaggcaaa
     7921 tataggttac tttttaatcg cagctcacgc tggcgcagca ctctttcatc actacattca
     7981 gaaagataat actctgttac gaatgatgca tcgccgcaaa taagtcgatc ccctgacaag
     8041 gaaatgtgaa ccatgtacat caacgcgtca taaatgttta cgcaagtgca caatgcatcc
     8101 tttctactat caacaaggag gagcagaaac tgtgtaaatc gtacaatggt aagacttatc
     8161 aaaaatacag gttaattttg gtatttaaac cttttttact tgttagtatg atacaaaggc
     8221 tttcaaaaaa gctgggaaat tcaacaactc ttggaaatta tggactttgt cccgcggctt
     8281 taatctgacg gccgcgttcc ttttttgact caattattga ccccttctct acgcaacttc
     8341 agttcccacc accaactttg cggcagtttc ataggatgaa tgtctaaaag aataatggtg
     8401 agcgataaga aaacgactga ataactgcac attttcgctc gaaagcttcc tgtcagatcc
     8461 atagcgaatc aagtgctgaa tgtcacagta tcgaacagaa aacagtgacg atctaaccct
     8521 tcaagaatat tctacaattg ttctgtttag gaaaagcaag gcgggaagtc aggagataag
     8581 tcattgataa agtggcggag agagggggat ttgaaccccc ggtagagttg cccctactcc
     8641 ggttttcgag accggtccgt tcagccgctc cggcatctct ccgttcagat ggttgccatg
     8701 atgccaggaa atttggcatt ttaacagtcc ctgtccgtgc aattttgttc aagtgacgag
     8761 tttgcgagca aaacgatgat taagtggccc tggaaagtac aagaatcagc acatcaaact
     8821 gcccttccct ggcaggaagc actatcgatc ccccttttaa cgggtctgac ggaacaggaa
     8881 caaagcaaat tagtcactct tgccgaacgt tttttacagc aaaagcggct tgttccttta
     8941 cagggctttg aactggattc attaagaagc tgccggatag cacttctatt ttgcctaccc
     9001 gttctggagt taggactgga atggctggat ggttttcatg aagtcttaat ttatcctgcg
     9061 ccatttgtgg tcgatgatga atgggaagac gatatcggtc tggtgcataa ccaacgtatt
     9121 gttcagtcag gtcagagctg gcagcaaggg cctatcgttt tgaactggtt ggatatacaa
     9181 gattcttttg atgcatctgg ttttaacctg attattcatg aagtcgctca taagctggac
     9241 acccgtaacg gcgatcgcgc cagcggagtt ccctttattc cgttgcgtga ggttgctggc
     9301 tgggaacacg atcttcatgc tgcaatgaac aacattcagg aagaaatcga attggttggc
     9361 gagaatgcgg cgagcattga tgcttatgca gccagcgatc ctgctgaatg ttttgccgta
     9421 ctttctgaat atttctttag cgccccagaa ctttttgctc ctcgtttccc ttcattgtgg
     9481 caacgtttct gccaatttta tcaacaagat cctttgcaga gactgcatca cgctaatgat
     9541 acagactcgt tttcggcgac gaatgttcat taattaacaa ctttgcagat taattaacca
     9601 attgaaatga cttatgaaat ttagtgttga cagacaaggt accgctaagt aatatgtgcc
     9661 ccgttcacac gattcctctg tagttcagtc ggtagaacgg cggactgtta atccgtatgt
     9721 cactggttcg agtccagtca gaggagccaa attctaaaaa ttcgcttttt tagcgcaatg
     9781 tcactgacct tagttgaaca ttgtttttta acggatagcg ggtttttaac accttaagcg
     9841 ccctcgacct ttatggttga gggcgttttg ctatgaacgc catcaccatt ttcccctcga
     9901 ttataaaact tgagttattc agtagtctcc cctcttgcaa ctcacaccca aaactgccta
     9961 acgaaaagtt attaattttc aatcgtattg ctatcagtat ttacattttt tcgctgtgct
    10021 agaaagggcg catttatgtt agctcgttca gggaaggtaa gcatggctac gaagaagaga
    10081 agtggagaag aaataaatga ccgacaaata ttatgcggga tgggaattaa actacgccgc
    10141 ttaactgcgg gtatctgtct gataactcaa cttgcgttcc ctatggctgc ggcagcacaa
    10201 ggtgtggtaa acgccgcaac ccaacaacca gttcctgcac aaattgccat tgcaaatacc
    10261 aatacggtgc cctacaccct tggagcgctg gaatcggccc aaagcgttgc cgaacgtttc
    10321 ggtatttcgg tggctgagtt acgcaaactc aaccagtttc gtacgtttgc tcgaggtttt
    10381 gataatgtcc gccagggtga tgaactggat gtcccggcac aagttagtga aaaaaaatta
    10441 accccgccgc cgggtaatag cagtgacaac ctcgagcaac agatagccag tacttcacag
    10501 caaatcgggt ctctgctcgc cgaagatatg aacagcgagc aagcggcaaa tatggcgcgt
    10561 ggatgggcct cttctcaggc ttcaggcgca atgacagact ggttaagccg cttcggtacc
    10621 gcaagaatca cgctgggcgt ggatgaagat tttagcctga agaactccca gttcgatttt
    10681 ctccatccgt ggtatgaaac gcctgataat ctctttttca gtcagcatac tctccatcgt
    10741 actgacgagc gtacgcagat taacaacggc ttaggttggc gtcatttcac tcccacatgg
    10801 atgtcgggca tcaacttctt tttcgaccac gatcttagcc gttaccactc ccgcgccggc
    10861 attggcgcgg agtactggcg cgactatcta aaattaagca gtaacggcta tttgcgactg
    10921 accaactggc gcagcgcacc tgaactggac aacgattata aagcacgccc ggccaatggc
    10981 tgggatgtac gcgcagaaag ctggctaccc gcctggccgc accttggcgg taaactggtc
    11041 tatgaacagt attatggcga tgaagtggcc ctgttcgata aagacgatcg gcaaagtaat
    11101 cctcatgcca taaccgctgg acttaactat acccccttcc cgctgatgac cttcagcgcg
    11161 gagcaacgcc agggtaaaca gggcgaaaat gacacccgtt ttgccgtcga ttttacctgg
    11221 caacctggca gcgcaatgca gaaacagctt gacccgaatg aagtcgctgc acggcgtagc
    11281 cttgcaggca gccgttatga tctggtggat cgcaacaaca atatcgttct ggaatatcgc
    11341 aaaaaagaac tggttcgcct aaccctgaca gaccccgtga cagggaagtc aggagaagtg
    11401 aaatcactgg tttcgtcgct acaaaccaaa tatgccctga aaggctataa cgtcgaagcc
    11461 accgcactgg aagctgccgg tggcaaagtg gtcacaacgg gtaaagatat tctggttacc
    11521 ctgccggctt accggttcac cagtacgcca gaaaccgata acacctggcc gattgaagtc
    11581 accgccgaag atgtcaaagg caatttgtcg aatcgtgaac agagcatggt ggtcgttcag
    11641 gcacctacgc taagccagaa agattcctcg gtatcgttaa gtacccaaac attgaacgcg
    11701 gattcccatt caaccgccac actgactttt attgcgcatg atgcagcagg taatcctgtt
    11761 gtcgggctgg tgctctcgac gcgtcacgaa ggtgttcagg acatcaccct ttctgactgg
    11821 aaagataatg gtgacggaag ctatacccag atcctgacca caggagcgat gtctggcacg
    11881 ctgacgctga tgccacagct gaatggtgtg gatgcggcta aagcccccgc cgtggtgaat
    11941 atcatttctg tttcgtcatc ccgaactcac tcgtcaatta agattgataa ggaccgttat
    12001 ctctccggca atcctatcga ggtgacggta gaactgagag atgaaaatga caaacctgtt
    12061 aaggaacaaa aacagcaact gaataacgca gtcagcatcg acaacgtgaa accaggagtc
    12121 actacagact ggaaagaaac cgcagatggc gtctataagg cgacctatac cgcctatacc
    12181 aaaggcagtg gacttactgc gaagctattg gaatgaagat ttgcataccg ctggatttat
    12241 catcgacgcc aacccgcagt cagcgaaaat tgcgacatta tctgccagca ataatggtgt
    12301 gctcgccaat gagaatgcag caaacaccgt ctcggtcaat gtcgctgatg aaggaagcaa
    12361 cccaatcaat gatcataccg tcacgtttgc ggtattaagc ggatcggcaa cttccttcaa
    12421 caatcaaaac accgcaaaaa cggatgttaa tggtctggcg acttttgatc tgaaaagtag
    12481 taagcaggaa gacaacacgg ttgaagtcac ccttgaaaat ggcgtgaaac aaacgttaat
    12541 cgtcagtttt gtcggcgact cgagtaccgc gcaggttgat ctgcagaagt cgaaaaatga
    12601 agtggttgct gacggcaatg acagcgccac aatgaccgcg accgtccggg atgcaaaagg
    12661 caacctgctc aatgacgtca tggtcacttt caatgttaat tcagcagagg cgaaactgag
    12721 ccaaaccgaa gtgaatagcc acgacgggat cgccacagct acgctgacca gtttgaaaaa
    12781 tggtgattat agggttacgg cctctgtgag ctctggttcc caggctaatc aacaggtgaa
    12841 ttttatcggt gatcaaagta ctgctgccct gaccctcagt gtgccttcag gtgatatcac
    12901 cgtcaccaac acagctccgc aacatatgac tgcaaccttg caggataaaa atggcaaccc
    12961 actaaaagat aaagaaatca ccttctctgt gccaaacgac gtcgcaagta agttctcgat
    13021 tagcaacgga ggaaaaggca tgacggatag taacggggtt gcaatcgcct ccctgaccgg
    13081 cacgttagcg ggcacgcata cggttaccgc aacgctgggt aataacaata ccagtgattc
    13141 gcagccggta acatttgtgg cggacaaagc ctcggctcag gttgtcctgc agatatcaaa
    13201 agatgagatc acaggtaatg gcgtcgatag cgcaacgcta actgcaacgg ttaaagatca
    13261 gttcgacaat gaggtgaata atcttccggt aacattcagc tcagcctctt caggactcac
    13321 cctgaccccg ggagtaagta ataccaatga gtctggcatc gcgcaggcca ctctcgcagg
    13381 cgttgccttt ggtgagcaga cggtcactgc atcactggct aataatggtg ccagcgacaa
    13441 caaaactgtg cattttattg gcgacacagc ggcggcaaaa attatcgagt tgacgcctgt
    13501 cccagacagc ataatcgccg gtaccccgca gaacagctcc ggcagcgcca tcaccgccac
    13561 agtcgttgat aataatggct ttccggtgaa aggtgtgact gtgaacttca ccagcagaac
    13621 aaactctgcc gaaatgacga atggcggcca agccgtaacg aacgaacagg gtaaggctac
    13681 cgtcacttat accaataccc gctcctcgat agaatcagga gcgagaccgg ataccgttga
    13741 ggccagtctg gaaaatggta gctccacgct tagcacatca attaatgtca acgctgatgc
    13801 gtctacggca catctcacct tgctacaggc actttttgat acagtctccg caggcgagac
    13861 aaccagtctg tatattgagg tgaaggataa ttacggcaac ggtgtccccc agcatcaggt
    13921 gaccctcagc gtttcaccaa gtgaaggcgt gacccccagt aataacgcta tatatactac
    13981 caaccacgac ggcaattttt acgcaagctt taccgctaca aaagccgggg tttatcaagt
    14041 gacggcaacc ctcgaaaatg gcgattcgat gcaacaaaca gtgacctatg tgccgaacgt
    14101 cacgaatgcc gaaatcacgc tggcagcctc gaaggatccg gtgattgccg acaataacga
    14161 tctcacgaca ctaacagcaa cagtcgctga tacagagggc aatgcgatag ccaacactga
    14221 agtaacattt actctgccgg aagatgttaa ggcgaacttc acgctgagcg atggcggtaa
    14281 agcgattact gatgctgaag gcaaagcgaa agtcacgctg aaaggtacaa aagcaggcgc
    14341 tcatactgtt acagcatcga taactggcgg taagagtgag cagttggtgg tgaactttac
    14401 tgcggatacg ctcactgcgc aggttaatct taacgttacc gaggacaatt ttatcgctaa
    14461 taacgtcggg atgaccaggc tgcaggcaac agtgactgat ggaaacggca acccgttagc
    14521 caatgaggcg gtgacattca cgctaccggc agatgtgagc gcaagcttta ctctcggaca
    14581 aggcggttcc gccattactg acatcaacgg caaggctgaa gttacactga gcggtacaaa
    14641 atccggcacc taccccgtga cagttagcgt gaacaattat ggtgtcagtg atacgaaaca
    14701 ggtgactttg attgccgatg ctggtaccgc aacactagcc tccttaacct ctgtatactc
    14761 attcgtcgtc agcacgaccg agggcgcgac catgactgca agcgtcactg acgctaacgg
    14821 caacccggta gaaggcataa aagttaattt ccgcggaacc tccgtcacgc taagcagcac
    14881 cagcgttgaa acggatgatc ggggtttcac tgaaattctt gtgacaagca ccgaagtcgg
    14941 actgaaaaca gtttcagcct ctctggcaga taaacctact gaagtcatat cgcgattact
    15001 gaatgcaaaa gcagatatta attctgcaac gattaccagt ctggagatac ctgaaggtca
    15061 ggtcatggtc gcacaagacg tagcagttaa agctcacgtc aacgaccagt ttggcaaccc
    15121 gattcttaat gaatctgtaa cattcagtgc agagccacca gagcacatga ccatcagcca
    15181 aaatattgtc tctactgata cgcatggtat agccgaggtc tccatgacgc ccgaaagaaa
    15241 cggttcgtat atggtgaaag catccctggc gaatggagcc tcacttgaga aacaactgga
    15301 ggctattgat gaaaaactga cactctcggc gtccagcccg cttatcggtg tcaattcccc
    15361 aacaggtgca actctgacgg caacgctaac ttctgcaaat ggcactccag tggagggtca
    15421 ggtcatcaac tttagcgtaa cgccagaagg tgcgacgtta agtggcggaa aagtgagaac
    15481 taactcttct ggtcaggctc cagtcgttct gaccagcaat aaagtcggta catatacggt
    15541 gactgcatcg ttccataacg gcgtaacaat acagacacag acaaccgtga aagtcactgg
    15601 caactcaagc accgcccatg ttactagctt tatcgctgat ccatcgacta tcgccgccac
    15661 caacagtgat ttaagtacct taaaggcaac ggttgaggat ggcagtggta acctgatcga
    15721 aggtctcact gtgtactttg ccttaaaaag cggctctgcc acattaacgt cattaacagc
    15781 ggtgaccgat caaaacggaa tcgcgacaac aagcgtgaaa ggagcgatga caggtagcgt
    15841 cacggtaagc gcagtcacga ccgctggtgg aatgcaaaca gtagatataa cgctggtggc
    15901 aggcccagca gacgcctcaa agtcagtcct taagaacaat cggtcatcat tgaaaggaga
    15961 ctttaccgat agtgctgagc tacatcttgt tctgcacgat atatcaggca atccgatcaa
    16021 agtttctgaa gggctggaat ttgtacagtc aggtaccaac gtgccctatg tgcaagttag
    16081 tgcaattgac tacagtaaaa atttctcagg cgagtacaaa gccactgtta caggaggcgg
    16141 agagggtatc acaacgctga tccctgtatt gaatggtgtt catcaagctg gtctgagtac
    16201 cacaatacaa ttcactcgcg cagaagacaa aataatgagc ggtacagtat cagtcaatgg
    16261 tactgaccta ccgacaacta cattcccttc gcaggggttc accggggcgt attatcagtt
    16321 gaataatgac aactttgccc caggaaaaac ggcggctgat tatgagtttt caagctctgc
    16381 ctcctgggtc gatgttgatg ctaccggtaa agtgacattt aaaaatgtcg gcagcaattc
    16441 ggaaaggatt acggcgacac caaaatcagg aggccctagc tatgtatacg aaatccgtgt
    16501 gaagagttgg tgggtgaacg ccggcgaggc tttcatgata tacagccttg ctgaaaattt
    16561 ttgcagcagc aatggctaca cgctccccag agcaaactat ttaaaccaca gtagttcccg
    16621 aggcatcggg tcactgtaca gtgaatgggg agatatgggg cattacacga ctgaagctgg
    16681 ttttcaatca aatatgtatt ggtcatctag tcccgcaaac tcaagcgaac aatacgtagt
    16741 ttccctggca acaggtgatc aaagcgtatt tgaaaagctt gggtttgctt atgcgacatg
    16801 ttataaaaac ctctgatttt ccgttataat ttcttaaaga gtgctccatt cggagctctc
    16861 ttttttacat tccctctata gagcttgccc ttctctcaca gaaaatgaat aaataaaaat
    16921 gcggcaccgc cagaattgcg ttcgatgtga ctatgatcgt atccggtgag tactgctttg
    16981 attcaaaggc tggttttctg tgatgattga ccagcctcag agtattgatg acaccaccgt
    17041 tcataacaga tcggcattac tatgcataag tactgtctca tattctcgcc aaaacacttt
    17101 ttaatgcctt tgcgaattca tccagactta tattgtcaat tttttgccca acgacgactg
    17161 actccgagac atcggccatt gcgggccatc gcgttttgta at
//
LOCUS       DAKISA010000094        17087 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.094, whole genome shotgun sequence.
ACCESSION   DAKISA010000094 DAKISA010000000
VERSION     DAKISA010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17087
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.094"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..908
                     /locus_tag="OVW45_003203"
     CDS             <1..908
                     /locus_tag="OVW45_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCV5527580.1"
                     /translation="NYSPAALIEKLKGFDSFTMADWIERYKTILIRRKVSRNTYKIRA
                     NQLETIKEKLGEILLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGR
                     ISQNPVTPTRAPKIVVTRERLKLKIYNCIREAADQLPAWFPLAMDLALVTGQRREDIT
                     NMRFSDIYDDRLHVRQIKTGMMIAIPLSLSLPVAGLRLGTVVERCRLVSRGDYLISAG
                     IRKNSPDGSIHPDGLTKKFVAARKFTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQ
                     RLLGHTSEKTTKMYLDEREKTYLLL"
     gene            complement(1301..1642)
                     /locus_tag="OVW45_003204"
     CDS             complement(1301..1642)
                     /locus_tag="OVW45_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCV5527581.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(1655..2527)
                     /gene="copD"
                     /locus_tag="OVW45_003205"
     CDS             complement(1655..2527)
                     /gene="copD"
                     /locus_tag="OVW45_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HCV5527582.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGMGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMCFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQEEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(2531..2905)
                     /gene="yobA"
                     /locus_tag="OVW45_003206"
     CDS             complement(2531..2905)
                     /gene="yobA"
                     /locus_tag="OVW45_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCV5527583.1"
                     /translation="MASTARSLRYALAILTTSLVTPFVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3044..3274
                     /gene="holE"
                     /locus_tag="OVW45_003207"
     CDS             3044..3274
                     /gene="holE"
                     /locus_tag="OVW45_003207"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCV5527584.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3376..4032
                     /locus_tag="OVW45_003208"
     CDS             3376..4032
                     /locus_tag="OVW45_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HCV5527585.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            4056..4718
                     /gene="exoX"
                     /locus_tag="OVW45_003209"
     CDS             4056..4718
                     /gene="exoX"
                     /locus_tag="OVW45_003209"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCV5527586.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(4715..6775)
                     /gene="ptrB"
                     /locus_tag="OVW45_003210"
     CDS             complement(4715..6775)
                     /gene="ptrB"
                     /locus_tag="OVW45_003210"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCV5527587.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPCENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTDERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(6984..7643)
                     /locus_tag="OVW45_003211"
     CDS             complement(6984..7643)
                     /locus_tag="OVW45_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCV5527588.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(7970..8326)
                     /locus_tag="OVW45_003212"
     CDS             complement(7970..8326)
                     /locus_tag="OVW45_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCV5527589.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8393..8683)
                     /gene="yebG"
                     /locus_tag="OVW45_003213"
     CDS             complement(8393..8683)
                     /gene="yebG"
                     /locus_tag="OVW45_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HCV5527590.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            8817..9995
                     /gene="purT"
                     /locus_tag="OVW45_003214"
     CDS             8817..9995
                     /gene="purT"
                     /locus_tag="OVW45_003214"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCV5527591.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10051..10692)
                     /gene="kdgA"
                     /locus_tag="OVW45_003215"
                     /gene_synonym="eda"
     CDS             complement(10051..10692)
                     /gene="kdgA"
                     /locus_tag="OVW45_003215"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCV5527592.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(10729..12540)
                     /gene="edd"
                     /locus_tag="OVW45_003216"
     CDS             complement(10729..12540)
                     /gene="edd"
                     /locus_tag="OVW45_003216"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCV5527593.1"
                     /translation="MNPQLLCVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSLGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEQ
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(12775..14250)
                     /gene="zwf"
                     /locus_tag="OVW45_003217"
     CDS             complement(12775..14250)
                     /gene="zwf"
                     /locus_tag="OVW45_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCV5527594.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            14587..15456
                     /gene="hexR"
                     /locus_tag="OVW45_003218"
                     /gene_synonym="yebK"
     CDS             14587..15456
                     /gene="hexR"
                     /locus_tag="OVW45_003218"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCV5527595.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            15584..17026
                     /gene="pyk"
                     /locus_tag="OVW45_003219"
     CDS             15584..17026
                     /gene="pyk"
                     /locus_tag="OVW45_003219"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCV5527596.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
BASE COUNT         4282 a         4333 c         4212 g         4260 t
ORIGIN      
        1 aaaactactc tcccgctgcc ttaattgaaa aacttaaagg gttcgactca tttactatgg
       61 cagactggat tgaacgttac aaaacgattc ttataaggag aaaagtgtcc agaaatactt
      121 ataaaattcg ggcaaatcaa ctggagacaa taaaagaaaa attgggagag attttactga
      181 cagaaataac cactcgccat attgccgagt ttcttgattt gtggattgaa ggagggaaaa
      241 acacaatggc aggatcaatg cgttctgtgt tgtctgatat gtttcgcgag gccattgttg
      301 aaggacgtat atctcaaaat ccagtcacgc caacaagagc accgaaaata gtagttacaa
      361 gagaacgact gaaactaaag atatacaact gcatcaggga ggcagcagat caacttccgg
      421 catggttccc attagctatg gatttagccc ttgtaacagg acaacgtcgc gaagacataa
      481 cgaatatgcg gttcagtgat atttatgatg atcgtctcca cgtcaggcaa attaagacag
      541 gaatgatgat tgctatcccc ctgtcactca gccttcctgt cgctggtcta cggcttggta
      601 cagtagttga acgatgccgc ctggtaagcc ggggagatta tctaatcagt gccgggatta
      661 gaaaaaacag ccctgacggc agcattcacc cggatggcct gacaaaaaaa tttgtcgcag
      721 ccagaaaatt cacaggtatc cagttcagtg aaaacccacc aacttttcac gagatcagaa
      781 gcctggctgg acgattgtac aaagaaacat gtggagaaga atttgctcag cgtctacttg
      841 gccacacatc ggagaagaca acaaaaatgt atcttgatga gagagaaaaa acgtacttac
      901 tgctctgatt ttaacgtaaa tggattgtta aatgtatttt ggttgtgata taaccaaaaa
      961 gaccggaata cagaaattcg agtaaatttc ggggaatttc gggggagaca tttgcaacta
     1021 attgatttca aatgcaatta aaaaaagacc gaatacgatt cctgtattcg gtccagggaa
     1081 atggctcttg ggagagagcc gtgcgctaaa agttggcatt aatgcaggct tagttgcctt
     1141 gccctttaag aatagatgac gacgccaggt tttccagttt gcgtgcaaaa tggtcaataa
     1201 aaagcgcggt ggtcatcagc ttaaatgtta aaaaccgccc gttctggtga aagaactgag
     1261 gcggtttttt tattggaaat caaaaggcta ttttaggtaa ttaacagagt ttttcagctc
     1321 gttctataaa cggtgccaga ctcatttttt cgccgggatt gttaggatca tcaatctgaa
     1381 tcaccgaaat gggttgggca ttggtcttcc cactggcaac ttccttttgt gcgatatcgt
     1441 ttaaaggata ctgcacgagg gtactcggat taataacata caaagcatta cccggtcggc
     1501 aagtcagcat cacctcttcg cgattaaacg cccatttgtc tttacccact tcaaaacgac
     1561 tgacggtaat cacctgcggt gcagccagcg ccgctgcaga actggtgagt aacagaaacg
     1621 ccagaatact ttttttcatc attttcgtaa tccatcaaaa aggttcgaga gtcgcaagca
     1681 ggctgatgat caccagcact accgcaccta tcgcccattc gagtttagtc atccacacaa
     1741 accagggtgc cgcacgatct tcctcctgcc tcatgcgcgg tacaagaaca taacgattag
     1801 ccagcgcaat gaccaccata atcatcacca ggatggcttt gagcaataag agttggcccc
     1861 agtaagtcgt aagcgtgggg ggaaatccgg taatcaacaa tgcattaagc acgccgcttg
     1921 ccagtacgcc gatcaccgca aaatgcccgc accatgaaaa acacatcagc gcctgaatag
     1981 cctggtgtcg ccaacggcct ttgatgagtt gcatacacca gagcaccggc agtaatcccc
     2041 caaaccacgc cgccgcacaa atcaggtgaa tcgcgtgatt agtctgatgg attttcgccg
     2101 ttaccccttc attcagtgtc gcatgcccca ttcccgccag cagaataaat tgcgcggtag
     2161 tgagcataaa cagcaaacgt ggcatattcc gcggttgcat aagggcgacg atcaacgtta
     2221 cgagggcgag aacaatttgc cataaccaga cgccaccaaa ctgcgtttgc aaaacagcct
     2281 gccaaatgtt tggcgaaaat acatctgtcc atcccgttcc catcaacccg ccctgaacgg
     2341 cgagcattgc agtggcactg atcaaactcc agacggcagc gtgctgttgt aaacgcagaa
     2401 aacgcttcgc cagcaagcga cgaatcgtca agggggccag ccaggcaccg tacattgcga
     2461 agccaaaaac cagcatcagc gaggtgaaat ggataaatcg cagcgcgatc caggtaaacg
     2521 ccagcatgat ttatttcacg ctaaaggtgt agtgtccttt cgttttatgc ccgtccaccg
     2581 aaacaacatg ccagtcaacg gtgtaggttc cgggtttcag cgaatcggcc agtggaacaa
     2641 tcagttgctt ttggtcctgc tcatttcgct tcgccggtaa tgttttaata ttttcgtttt
     2701 ttggccccgt gatttttgca ccactgaatc ctgtttccac accttccgag aagtttaaag
     2761 tgattgcctg cggtgcagct gtcacttgcg cgtttgccgc aggatactga tgcgttaaat
     2821 gcgcatgtgc ccagacgaaa ggtgtcacca acgaagtggt caggattgcc agcgcgtagc
     2881 gaagggagcg tgcagttgaa gccatattat ctattccttt ttgtaataac ttttttacag
     2941 agcataacct tgtctaatgt ctgagtcgag gatcatcaat tccggcttgc catcctggct
     3001 cactcttagt aacttttgcc cgcgaatgat gaggagatta agaatgctga agaatctggc
     3061 taaactggat caaacagaaa tggataaagt gaatgtcgat ttggcggcgg ccggggtggc
     3121 atttaaagaa cgctacaata tgccggtgat cgctgaagcg gttgaacgtg aacagcctga
     3181 acatttgcgc agctggtttc gcgagcggct tattgcccac cgtttggctt cggtcaatct
     3241 gtcacgttta ccttacgagc ccaaacttaa ataaaactta tacagggtta cactttctta
     3301 cataacgcct gctaaattat gagtattttc taaaccgcac ccataatttg cagtcatttt
     3361 gaaaaggaag tcattatgtc gttctggaaa gttgcagcag cgcaatatga gcctcgtaaa
     3421 acctcactca ccgaacaagt agctcatcat ctggagtttg tgagggccgc cgccaggcaa
     3481 caatgtcagc tgttagtttt tccatcgctt tctttactgg ggtgtgatta ttcgcgacgt
     3541 gcccttcctg ccccacccga tctctcactg ttagacccgc tttgttatgc cgcaacgacc
     3601 tggcgaatga ccatcattgc cggccttccc gttgaatata acgatcgctt tattcgtgga
     3661 attgcggtgt tcgcaccgtg gcgaaaaacg cctgggatct accatcagag tcatggtgca
     3721 tgtctgggcc gtcgttccag aaccatcacg gtggtcgatg aacaaccgga aggtatggat
     3781 atggacccga cctgttcact gtttacgaca gggcaatgtc tgggagaacc cgacctgctg
     3841 gcgtcggccc gtcgcttaca atttttttca catcagtact cgattgctgt gctgatggca
     3901 aatgcccgtg gtaacagtgc actatgggat gaatatggtc gtctcatcgt tcgcgccgat
     3961 cgtggttcat tattgttagt cggtcagcgt tcatcacagg gttggcaagg cgatatcatt
     4021 ccattacgct aggctttttc ggcctggagt atgccatgtt gcgcattatc gatacagaaa
     4081 cctgcggttt gcagggaggg atcgttgaga ttgcctctgt tgatgtcatt gacggaaaaa
     4141 tcgtcaaccc catgagccac ctggtgcgcc ccgatcgtcc tattagtcca caagcgatgg
     4201 cgattcatcg catcaccgaa gccatggtcg ccgataaacc gtggattgaa gatgtgatcc
     4261 cacactatta cggcagtgaa tggtatgtcg cgcacaacgc cagctttgac cgccgcgtac
     4321 tgcctgagat gcccggtgag tggatttgca ccatgaaact ggcccgtcgc ttgtggccag
     4381 gaatcaagta cagcaatatg gcgttatata aaacacggaa gctcaatgta cagacgccgc
     4441 cgggcctgca tcatcaccgc gcgttgtatg actgttatat caccgccgcg ttgcttatcg
     4501 atattatgaa cacctccggc tggacggcag aacagatggc cgatatcacc ggacgtccgt
     4561 cgttgatgac gaccttcacc tttggcaaat accgtggcaa agcggtttcc gacgttgccg
     4621 aacgcgatcc gggctatctg cgctggttat ttaataacct ggacagcatg agcccggagc
     4681 tgcgtttaac actgaaacat tatctggaaa atacttaatc cgcaggcgta gcgggtaatg
     4741 ttccctgcgc cagcgcgacc agaaaagcat attccatcgc tacgccttcg tacgatttaa
     4801 agcggccaga tttaccgcca tggcctgagt ccatgtcggt acagagcaat aaaagatggt
     4861 tatcggtttt cagctcgcgc aatttagcga cccattttgc cggttcccaa tattgcacct
     4921 gagaatcgtg taaaccggtc gttaccagta aatgcggata agcctgtgcg gtgacgttgt
     4981 catacgggct gtagcttttc atgtactcgt aatattgcgg atcctgcggg ttaccccact
     5041 cttcaaactc accagtggta agaggaattg attcatcaag catcgttgtt acaacatcaa
     5101 caaacggtac ctgggcgata acgccgtgga ataattccgg gcgttgatta attgcaacgc
     5161 ccatcaacat gccccccgca ctcccgccca tcgcataaca aagcgaagga gagccatagc
     5221 ccagttttaa caatgcatcg caggcatcaa gataatcatt aaacgtattt ttcttcttca
     5281 gaaattttcc gtcttcgtac cattgttgcc ccagctcacc accgccgcga acatggacaa
     5341 tggcgtagac aaagccacga tctaacaaac tcaagcggct aaaactgaaa tcggcatcaa
     5401 tacttgcgcc gtaagaacca tagccataca ccagcaacgg gttgtgtcct ttgcgaaaat
     5461 gtttgcgatg gtagaccaac gaaaccggaa cttcgacgcc atcacgggcg actatccaca
     5521 ggtgttcact gcggtaattc gccgcatcaa aaccaggaac ttccgtttgt tttaatacac
     5581 gacgctcatc ggtatccata tccagttcaa acaaagtgtc tggtgtggtc atggaagaat
     5641 aaccataacg caatcgcgcg gtttcaggtt ctggattgta ggcaatccag gtcacatagg
     5701 ctggatcatc aaaggcaata ccaatgactt cccgggtctt gcggttaatt tggcgcaaac
     5761 tggttaaccc gcgctgacgc tcttcaacca ccagccagtc ggtaaacagc gtaaaccctt
     5821 ccagcatgat gttttcgcat ggcggaatta actcttccca ctgttgctca tcacgcatac
     5881 gggtacggta taagccaaag tttttgccgt ggcggttgga acgcagataa aaacgatgct
     5941 ggtagtgatc aaggctgtat tcgtgatctt tgcggcgcgg cagaaaaaca aacggctcgg
     6001 catcggccat ttccgcgtcc agcaggcgaa cttcactggt ggtggcgctg gccaaatgaa
     6061 tgactacata gtgcttcgag gtcgttttat gcaggctgac gtaataggta tcgtcttttt
     6121 cttcgtagat cagtttatct tgcgatgctg gcgtaccgat ggcgtgacgc cagacctgat
     6181 aaggcagcag cgtcaccgga tgcttgcgaa catagtagaa agtccaggag tcatttgccc
     6241 agacaaagct gggttcaacg ttatccagca gttccgggta ccagttacca gtttccagat
     6301 tacgaaaacg aatgccgtac tggcgtcggg aaagaaaatc ttctgccagc gccataatgg
     6361 tgttatcggg cgtaatcgcc attccgccca tcgaataaaa ctcactatga gctgcgcgct
     6421 tattggcatc gagcaatatt tcccactcat cccactcttc actgaatgcc gattgacgct
     6481 ggtagatagc atattcacag cctggttcat aaatatgccg atagcggtag ccatttttga
     6541 tgtagggcgc agaaacttct cgttgcggaa tgcggtcgat gatttccttt aagatgcgat
     6601 cctgcaaggc ttgttgtgag gccatcaccc gatgaccgta actattttct tgttgcaggt
     6661 agtccaggac ttctggctga gaacgcgtat catcccgcag ccagtagtaa ttatcgatgc
     6721 gcgtatcgcc atgaagcgtc atggcgtggg gaatgcgggc ggcttttggt agcatgttat
     6781 tgttctttct ggttgaaaca tcttataagg gtggcaaaac tcaccgggga tgcaagcgaa
     6841 acaggggagt cattgcttag atgatgacag gtaatggcac ggatatcgaa tgttatgcag
     6901 acagagaaac tcagcctgtt caaatggctg tgcggttctg gatagcccga aatagtcaac
     6961 ttcaggctat ccagagagcg gaattattcc gccaaagtgc gtttttgctg ttcgagatcg
     7021 cgttcaatgc cgtcacgaac atcctggggg attttcagcg cgtcacccag tgcattcagg
     7081 taactgcgtt ccataaaatg gtcaatatca atagccgcgc aactcaggaa atagatttcc
     7141 agcgcctctt cttcattgcg gaccccggta gccaggcgtt gtggatccag cggttgttcg
     7201 attgcctgct caatgagtac acgcccctgc tcttccacgc cggcttcacg caattgctgg
     7261 tcgattgccg cacgttcttt ggcatcaata tgaccatcac ttttagcggc aaagaccagc
     7321 gcaaggatca aacgttctgt acgctcatcc agcggcgtac tttgcgcgcc aaactgtggt
     7381 tcgtcctgat gcgccgcgcg aattttatct ttgtatttat tccacagcac cgtacccgct
     7441 accgctccgc cgccaaccag taacgcgttg gtgccatatt tcgtaagaag tttacgtgct
     7501 gatttatttg cgaccagcag cccagccagt ccgcctaatg cgcctggcac taacagtttg
     7561 cccaatccct gatccgcaga cgaggaggta gaagaactgc tttgcccaag cagggattgc
     7621 agttgattta accagttagc catatttgct cctcaataac catttttagt gcctgtagca
     7681 tagcggatga ggatgtcaga aaatgtatat cgagacaaaa gatgcgcaaa taagcggggc
     7741 ggtaggccgt tattcaaaga aattcgcgcc aggtaaggta tttactgtat atcaggtgat
     7801 aagggtattt taggtgaaag tagtttgtcg ttttgagtgg cattgtttga tgctgattgc
     7861 ccgatgcgac gctgttgcgt cttctcaggc ctacgattgt ggtgcgttct ggggcggaca
     7921 aggcactcgc gccgcatcca ccaacggtca atcgaattat cagaagccat taacgccgct
     7981 gatattccgc cattcccgct ttgcagtcca cgctgacctg gtaatgaata tcggcacttt
     8041 taccacgcac ggttagcggt actgaccatt tatcatcttt accctgaatg tcctgcaaac
     8101 tgacccaagc cacgggatcg gcctgaccga caattttttg atcatctgcc caacgcgcca
     8161 cgcgattttg ttgataatca cgttttacgc tcgcggcaat tccggcagca tccagacctt
     8221 cacactttgg gaaagtgacc gacttgctgg tttcattatt ggcagcgaaa actgatgcgc
     8281 aggcagaaac caacaacagc cctaaaaacg cccctctttt tttcatgttt ttctccatag
     8341 cacaatgatt caggagaaag catggtacaa attgtcagga gcgcaagttg cttcaggccg
     8401 cgtgtgaggc atcttcctct tcggattcag caccgacgac ctgtggggac ggtaatttgc
     8461 cggttttcag aatggtgctc agcacatctt tttgttctgc cagccatagc gaaagggctt
     8521 cacgttgctc gtcttccatt tgcactggag aatttgtcag ccaggtgtca agcagatccg
     8581 ccgtatcaag cattttgtca taagcatcgg cttccttttt gctggtaaac gacattttct
     8641 cttcgccctc acgaatgact acgtatttaa cttcaaccgc catttgcagc ctctcataat
     8701 aactgtgatt ttatacagta tatttctttt cggttgagaa atcaacatca gcaataaaga
     8761 cacacgcaaa cgttttcgtt tatactgcgc gcggaattaa tcaggggata ttcgttatga
     8821 cgttattagg cactgcgctg cgtccggcag caactcgcgt gatgttatta ggctccggtg
     8881 aactgggtaa agaagtggca atcgagtgtc agcgtctcgg cgtagaggtg attgccgtcg
     8941 atcgctatgc cgacgcacca gccatgcatg tcgcgcatcg ctcccatgtc attaatatgc
     9001 ttgatggtga tgcattacgc cgtgtggttg aactggaaaa accacattat atcgtgccgg
     9061 agatcgaagc tattgccacc gatatgctga tccaacttga agaggaagga ctgaatgttg
     9121 tcccctgcgc tcgcgcaacg aaattaacga tgaatcgcga gggtatccgt cgcctggcgg
     9181 cagaagagct gcagctgccc acttccactt atcgttttgc cgatagcgaa agccttttcc
     9241 gcgaggcggt tgctgacatt ggctatccct gcattgtaaa accggtgatg agctcttccg
     9301 gcaaggggca gacgtttatt cgttctgcag agcaacttgc tcaggcatgg aagtacgctc
     9361 agcaaggcgg tcgcgccgga gcgggccgcg taattgttga aggcgtcgtt aagtttgact
     9421 tcgaaattac cttgctaacc gtcagcgcgg tggatggcgt ccatttctgt gcaccggtag
     9481 gtcatcgcca ggaagatggc gactaccgtg aatcctggca accacagcaa atgagcccgc
     9541 ttgcccttga acgtgcgcag gagattgccc gtaaagtggt gctggcactg ggcggttatg
     9601 gtctgtttgg tgtcgagctg tttgtctgtg gtgatgaggt gattttcagt gaggtctccc
     9661 ctcgtccaca tgataccggg atggtgacgt taatttctca agatctttca gagtttgccc
     9721 tgcatgtacg tgccttcctc ggacttccgg ttggcgggat ccgtcagtat ggtcctgcag
     9781 cttctgccgt tattctgcca caactgacca gtcagaatgt cacgtttgat aatgtgcaga
     9841 atgccgtagg cgcagatttg cagattcgtt tatttggtaa gccggaaatt gatggcagcc
     9901 gtcgtctggg tgttgcgctg gcgaccgctg agagtgttgt tgacgccatt gaacgcgcga
     9961 agcacgccgc cggacaggta aaagtacagg gttaaacccg gcaaaaaaac gctacaaaaa
    10021 tgcccgatcg tagatcgggc gttttgactt ttacagctta gcgccttcta cagcttcacg
    10081 cgccagctta gtaatgcggt cgtaatcgcc cgcttccagc gcatctgccg gaaccagcca
    10141 ggaaccaccg atgcacagca cgcttttcag cgccaggtag tcacggtagt tagccggaga
    10201 aataccaccc gtcgggcaga aacggacctg ggagaacgga cccgcgatcg cctgcagggc
    10261 tttcacgccg ccgttagctt cagccgggaa gaatttgaac tctttcaaac cgtagtccat
    10321 acccagcatc agttcggaaa cagtgctgat ccccggaatt agaggaatag tcccttcggt
    10381 agcagctttc agcagcggct cggtcagacc cgggctaatt gcgaactgtg cacccgcttc
    10441 agtgacttct gccagctgct gtggattcag caccgtaccg gcacccacaa tcgcttcagg
    10501 cacttctttg gcgatagcac ggatagcgtc aactgcacac tcggtacgca gagtcacttc
    10561 cagaacgcgc accccaccag caaccaacgc ttttgccatc ggcactgcgt gttccagttt
    10621 ttttaccacg ataaccggta caaccgggcc ggtggtcagg attgattctg cacttgtttt
    10681 ccagtttttc atcagagttt tctctcgcct gattacaaat ttgtcgtctt aaaaagtgat
    10741 acaggttgcg ccctgttcgg caccggacag tttttcacgc aaggcgctga ataattcacg
    10801 tcctgtcccc acgcgtgacg cgctcaggtc aggaatgtgc ggttcgcgag cagccagttc
    10861 cgcttcgtct accagcagcg tcagttcgcc tgtctgtcca ttcacacgaa tgatgtcccc
    10921 gtcgcgcact tttgccagca gcccgccatc gtaggcttct ggtgttacgt ggatagctga
    10981 cggcacttta cctgaagcgc cggagagtcg tccatcggta actaacgcaa ttttgaaaca
    11041 ccggtccaat aatacaccaa gtggcggcat gagtttatgt aattctggca ttccgttcgc
    11101 ttttggcccc tgatgacgga caacaacgac acaatcgcgg tccagcaaac ccgcttcaaa
    11161 ggccggcata acgtcatgct ggctttcaaa aacaaccgct ggcgcttcaa tcacctggtt
    11221 ctcaaccggc acggcagagg ttttcataac cgcacggccc aggttaccgc ttaacacttt
    11281 tgtcccacca tgatgagaga aaggttgttc gaaggaagcg atcacattgc tgtcgagtga
    11341 tttttccgcc ccttcccgcc agtcctgttc accattattc agccatggtt caagggtata
    11401 acgagacaga ccaaagcctg ccaccgtatt gacatcttca tgcagcaggc ctgctttgag
    11461 cagttcacgc accagaaccg gtacgccacc tgccgcctgg aagtggttaa tatcggccgg
    11521 accgttcggg tagagacgtg ccatcagcgg tacaacatca gaaaggtcag agaagtcatc
    11581 ccagttaatc tgaataccgg ccgcgcgcgc catcgccacc aggtgcatgg tgtggttagt
    11641 ggaaccaccg gtcgccagca gtgcaacgat accgttcacc accactttct catcgatcat
    11701 cttaccgatc ggcatccatt cattaccatt accggtcatg cgtgtaacct gacgcgcagc
    11761 ggcagcggtc aaagcatcgc gcagcggaga atccggatga acaaaagaag agcctggcaa
    11821 ctgcatcccc ataaattcca ccaccatctg gttggtgttg gcagtaccgt agaaagtaca
    11881 tgttcccggc gcatggtaag acgcggcttc tgactccagt aaggccatgc ggtccacttt
    11941 accttcggca taaagctggc gaatacgcac tttttcttta tttggcaaac cgcttgccat
    12001 cggtccagac ggcacaaaca ccgcaggcaa atgaccaagc gacagggctg ccatcgtcag
    12061 acccgggaca atcttgtcgc acacaccgag gaacagagca ccatcaaaca tgttatggga
    12121 cagccccacc gccgcagaca tcgctatcac ttcgcggctt agcagcgaca attccattcc
    12181 atcctgcccc tgggtgacac catcacacat cgccggaaca ccgcccgcaa cctgaccaac
    12241 cgcattcgct tcatgcaggg ctttacgaat gatttctgga tagtgttcat aaggctggtg
    12301 cgcggagagc atgtcgttat aggaggtgat gatggcgata ttgttacgca acatgctttt
    12361 caaagaggct ttgtcttctg gctggcaggc agcgaaaccg tgtgccaggt taccgcatgc
    12421 caactgcgaa cgatgaacgg tcgacgtttt cgcttgttct atccgggcga gataagcaga
    12481 gcgagtctcg cgcgaacgtt caatgattcg atttgttacg cataacaatt gtggattcat
    12541 aaaggctcct gaaattgagt tgtcagagca ggatgattca caacgcgttt cattcagagg
    12601 atttatgagt gaaacgcctg taaccggagc tcatagggca aaaacgtttc agtcagtgta
    12661 ataaaaaaag cctcgtgggt gaatccgcac gaggcctgaa agtgtaaaaa ttgttctaca
    12721 atctgtgcaa gatcatgtta ccggtaaaat aaccataaag gataagcgca gatattactc
    12781 aaactcattc caggaacgac catcacgggt aatcatcgcc accgaggcaa cgggtcccca
    12841 ggttccggcc tgatacggtt tcggcgcatc attgtccatc gcccacgcct cagtaatgga
    12901 gtctacccat ttccaggctt cttccacttc atcacgacgt acaaacagtg cctgaatacc
    12961 acgcatggtt tccagcagca gacgttcata ggcatccgcc agatgcgtct gattaaaggt
    13021 ttctgaatag ctcagatcca gcttggtgat ttgcaggtta tgtttgtggt caaggccagg
    13081 aactttattc agtacctgga tatccacgcc ttcatcaggt tgcagacgga tagtcagttt
    13141 attctgcggc agatcctgcc acgactcttt aaacagattc agttcaggtg ttttgaaata
    13201 gaccacgact tcagaacatt tggtcggcag acgtttacca gtacgcaggt agaatggcac
    13261 accggcccag cgccagttat caatgtcgac gcggatcgcc acgaaggttt ctgtattgct
    13321 gctcttgttc gcgccctctt cttccagata tcccggcact tttttgccct gggcgaagcc
    13381 cgcagtatat tgcccgcgta cggttttttc gcgtacgttg gagcggtcga tgcggcgcag
    13441 ggacttcagt actttcactt tttcatcgcg gatgctgtct gcgctcaggt cagatggcgg
    13501 agacatcgca atcatgcaga gaatttgtag caggtggttc tgaatcatgt cacgcatctg
    13561 accggcttta tcaaaatagc cccagcgccc ttcgatcccc acttcttctg ccacggtaat
    13621 ctcaacatga tcaatggtgc gattgtccca gttattcaca aacagggagt tagcaaaacg
    13681 cagcgccaac aggttcagca ccgtttcttt accaagatag tggtcgatac ggtaaacctg
    13741 gcactcctcg aagtattcgc caacctgatc attgatttcc tgcgaggtcg ccagcgacgt
    13801 ccccagcggt ttctccatga ctacgcgtgc cggtttagca ttcagttttg cctcgccaag
    13861 ccctttgcaa attgcgccaa aagtgctggg cggcatggca aagtagttaa tggtgatacg
    13921 atttttttga tccagcatcg cgccgagacg gttgaatgca gcagtgtcat tgacatcgag
    13981 attacaaaaa tccagacgtg cactcagggt gtcccataaa ccttcatcaa tggtttcttt
    14041 catgaaagtt tcgagcgcct cgcggacaac tttggtatat gccgctttat cccagtcagc
    14101 acgccctacg ccgataatcc gggtgtccgg gttgagctga ccggcttttt ccagttgata
    14161 cagggaaggc agcaatttac gacgcgcaag gtcgcctttc gcgccgaaaa tgaccaggtc
    14221 acaggcctgg gctgtttgcg ttaccgccat gtcattctcc ttaagttaac taacccggta
    14281 cttaagccag ggtatacttg taattttctt acggtgcact gtactgcttt tacgagcttg
    14341 cgaaaactgt aaacgcttat ccacccgtgc gattacggga aaagcgcgca aagtgcggca
    14401 aaactgataa aaaaatcatc gttttccatt cggtaaaaat ctgacactga tcatgttatg
    14461 aaaaaaataa caactttttt atctgctttt gtcattaaca cggcacacag gcgtaatatc
    14521 tgacaaaact gcatttcgat ttctttcagt gcggaaatcg tcattacccg tgagtctctt
    14581 tacatcatga atatgctgga aaaaatccag tctcagctgg aacatttgag caaatcagag
    14641 cgcaaagttg ccgaggtcat tctggcttcg cccgataacg cgatccattc gagtattgct
    14701 gctatggcac tggaagccaa tgttagcgaa ccgacggtga atcgtttctg tcgcagcatg
    14761 gacacgcgcg gttttcctga ttttaaactt catctggcac agagtctggc gaatggcact
    14821 ccctatgtta atcgcaatgt caatgaagat gacagcgttg aatcatacac agggaaaata
    14881 tttgagtccg caatggcaac gcttgatcat gtccgtcatt cactggataa atctgccatc
    14941 aaccgcgccg tcgacttgct cactcaggca aaaaaaatcg cctttttcgg attaggctct
    15001 tcagccgccg ttgcccacga tgcgatgaat aagttctttc gttttaatgt tccggtggtg
    15061 tactccgatg atatcgtgct gcaacgcatg agttgtatga attgtagcga cggagacgtg
    15121 gtggtgctga tttctcacac tggaagaaca aaaaatctgg tcgagctggc gcagctggct
    15181 cgcgaaaacg acgcaatggt gattgccctc acctctgcgg gtaccccgct cgcccgggaa
    15241 gcaacgctgg caattaccct cgacgtaccg gaagatactg acatttatat gcccatggtt
    15301 tctcgacttg cacagctgac cgtgatagat gtgctggcga caggatttac tttgcgacgc
    15361 ggtgcaaaat tcagagataa cttgaagcgg gtcaaggaag cgctgaagga atcgcgtttt
    15421 gataagcagt tacttaattt aagtgacgat cgctaaaaac gactgtcact gtcctaatct
    15481 tatacgacat ccgaatgaga ttaatttatc gccatcgcgg cgttatttca ttcggatttc
    15541 atgttcaagc aacacctggt tgtttcagtc aacggagtat tacatgtcca gaaggcttcg
    15601 cagaacaaaa atcgttacca cgttaggccc agcaacagat cgcgataata atcttgaaaa
    15661 agttatcgcg gcgggtgcca acgttgtacg tatgaacttt tctcacggct cgcctgaaga
    15721 tcacaaaatg cgcgcggata aagttcgtga gattgccgca aaactggggc gtcatgtggc
    15781 tattctgggt gacctccagg ggcccaaaat ccgtgtatcc acctttaaag aaggcaaagt
    15841 tttcctcaat attggggata aattcctgct cgacgccaac ctgggtaaag gtgaaggcga
    15901 caaagaaaaa gtcggtatcg actacaaagg cctgcctgct gacgtcgtgc ctggtgacat
    15961 cctgctgctg gacgatggtc gcgtccagtt aaaagtactg gaagttcagg gcatgaaagt
    16021 gttcaccgaa gtcaccgtcg gtggtcccct ctccaacaat aaaggtatca acaaacttgg
    16081 cggcggtttg tcggctgaag cgctgaccga aaaagacaaa gcagacatta agactgcggc
    16141 gttgattggc gtagattacc tggctgtctc cttcccacgc tgtggcgaag atctgaacta
    16201 tgcccgtcgc ctggcacgcg atgcaggatg tgatgcgaaa attgttgcca aggttgaacg
    16261 tgcggaagcc gtttgcagcc aggatgcaat ggatgacatc atcctcgcct ctgacgtggt
    16321 aatggttgca cgtggcgacc tcggtgtgga aattggcgac ccggaactgg tcggcattca
    16381 gaaagcgttg atccgtcgtg cgcgtcagct aaaccgagcg gtaatcacgg cgacccagat
    16441 gatggagtca atgattacta acccgatgcc gacgcgtgca gaagtcatgg acgtagcaaa
    16501 cgccgttctg gatggtactg acgctgtgat gctgtctgca gaaactgccg cagggcagta
    16561 tccgtcagaa actgttgcag ccatggcgcg cgtttgcctg ggtgcggaaa aaatcccgag
    16621 catcaacgtt tctaaacacc gtctggacgt tcagttcgac aatgtggaag aagctattgc
    16681 catgtcagca atgtacgcag ctaaccacct gaaaggcgtt acggcgatta ttaccatgac
    16741 cgaatcgggt cgtaccgcgc tgatgacctc ccgtatcagc tctggtctac caattttcgc
    16801 catgtcgcgc catgaacgta cgctgaacct gactgctctc tatcgtggcg ttacgccggt
    16861 gcactttgat agcgctaatg acggcgtagc agctgccagc gaagcggtta atctgctgcg
    16921 cgataaaggt tacttgatgt ctggtgacct ggtgattgtc acccagggcg acgtgatgag
    16981 taccgtgggt tctactaata ccacgcgtat tttaacggta gagtaatctc acctgccccg
    17041 gccgctgatg gccggggtat ttagcaacac ataaattagg actactt
//
LOCUS       DAKISA010000095        17057 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.095, whole genome shotgun sequence.
ACCESSION   DAKISA010000095 DAKISA010000000
VERSION     DAKISA010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17057
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.095"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            196..1149
                     /locus_tag="OVW45_003220"
     CDS             196..1149
                     /locus_tag="OVW45_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCV5527597.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(1190..2185)
                     /gene="pgl"
                     /locus_tag="OVW45_003221"
     CDS             complement(1190..2185)
                     /gene="pgl"
                     /locus_tag="OVW45_003221"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCV5527598.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTCWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            2320..2409
                     /locus_tag="OVW45_003222"
                     /pseudo
     CDS             2320..2409
                     /locus_tag="OVW45_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
     gene            complement(2410..3468)
                     /gene="modC"
                     /locus_tag="OVW45_003223"
     CDS             complement(2410..3468)
                     /gene="modC"
                     /locus_tag="OVW45_003223"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCV5527599.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(3471..4160)
                     /gene="modB"
                     /locus_tag="OVW45_003224"
     CDS             complement(3471..4160)
                     /gene="modB"
                     /locus_tag="OVW45_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCV5527600.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGEESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(4160..4933)
                     /gene="modA"
                     /locus_tag="OVW45_003225"
     CDS             complement(4160..4933)
                     /gene="modA"
                     /locus_tag="OVW45_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCV5527601.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSTTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(5099..5248)
                     /gene="acrZ"
                     /locus_tag="OVW45_003226"
     CDS             complement(5099..5248)
                     /gene="acrZ"
                     /locus_tag="OVW45_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCV5527602.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            5377..6165
                     /gene="modE"
                     /locus_tag="OVW45_003227"
     CDS             5377..6165
                     /gene="modE"
                     /locus_tag="OVW45_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCV5527603.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDIQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            6233..7705
                     /gene="modF"
                     /locus_tag="OVW45_003228"
     CDS             6233..7705
                     /gene="modF"
                     /locus_tag="OVW45_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCV5527604.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLNLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7966..8982
                     /gene="galE"
                     /locus_tag="OVW45_003229"
     CDS             7966..8982
                     /gene="galE"
                     /locus_tag="OVW45_003229"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCV5527605.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8992..10038
                     /gene="galT"
                     /locus_tag="OVW45_003230"
     CDS             8992..10038
                     /gene="galT"
                     /locus_tag="OVW45_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCV5527606.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            10042..11190
                     /gene="galK"
                     /locus_tag="OVW45_003231"
     CDS             10042..11190
                     /gene="galK"
                     /locus_tag="OVW45_003231"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCV5527607.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            11184..12224
                     /gene="galM"
                     /locus_tag="OVW45_003232"
     CDS             11184..12224
                     /gene="galM"
                     /locus_tag="OVW45_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCV5527608.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKSCPVNMTNHVYFNLDGEQSDVRNHKLQILAGEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            12427..13179
                     /gene="gpmA"
                     /locus_tag="OVW45_003233"
     CDS             12427..13179
                     /gene="gpmA"
                     /locus_tag="OVW45_003233"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCV5527609.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(13338..14390)
                     /gene="aroG"
                     /locus_tag="OVW45_003234"
     CDS             complement(13338..14390)
                     /gene="aroG"
                     /locus_tag="OVW45_003234"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCV5527610.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPVTENAANTVAHARKAIH
                     KILKGNDARLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            14706..15086
                     /locus_tag="OVW45_003235"
     CDS             14706..15086
                     /locus_tag="OVW45_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCV5527611.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQIGDGINNDVDTKTDGTTQ"
     gene            15200..16141
                     /gene="zitB"
                     /locus_tag="OVW45_003236"
     CDS             15200..16141
                     /gene="zitB"
                     /locus_tag="OVW45_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCV5527612.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVITLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(16138..16857)
                     /gene="pnuC"
                     /locus_tag="OVW45_003237"
     CDS             complement(16138..16857)
                     /gene="pnuC"
                     /locus_tag="OVW45_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCV5527613.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16895..>17057)
                     /gene="nadA"
                     /locus_tag="OVW45_003238"
     CDS             complement(16895..>17057)
                     /gene="nadA"
                     /locus_tag="OVW45_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCV5527614.1"
                     /translation="CAHCPWMAMNGLQSIAEALEQEGSNHEVLDERLRERALVPLNRM
                     LDFAATLRG"
BASE COUNT         4315 a         4469 c         4247 g         4026 t
ORIGIN      
        1 tatttttttc ataataatgc cctcaattta aaaacataac ttattgcgat atggttacat
       61 taagggcaaa gcatctctaa tgactttagc tcaacgatta ataacatttt ttcattaatt
      121 taagttggct aaatgcagtt aaaattttta acggccagcc acccaaaatc gtcgcttcca
      181 ttttccggag taataatgaa gcatgaatta tcaagtatga aggcatttgt catactggca
      241 gagtccagtt cgtttaacaa tgccgctaaa ttactcaata ttacgcaacc tgcattaacg
      301 cgaagaataa aaaagatgga ggaagattta catattcaac tatttgagcg tacaactcgc
      361 aaggttacgt taacaaaagc aggaaaaagg ttgctcccgg aggcgcggga attaataaag
      421 aaattcgatg aaacgctttt taatattcgt gatatgaatg cttatcatcg tggtatggtg
      481 acgttagcgt gtattccgac cgcagtattt tattttttac cgctggcaat cggtaaattt
      541 aatgagctgt atcccaatat taaagtgcgg attctggaac aaggcacgaa taattgcatg
      601 gaatcggtgc tgtgtaacga atcagacttt ggcataaaca tgaacaacgt cacaaattca
      661 tccattgatt ttaccccact ggtcaacgag ccgttcgtgc tggcctgtcg gcgtgaccat
      721 ccattggcca aaaagcagct tgtagaatgg caggagttag tgggttacaa aatgattggc
      781 gtacgctcct ccagcggcaa ccgactgttg atagaacaac agctggccga caagccctgg
      841 aagctggact ggttttacga agtgcgtcat ctttcgacgt cattaggact ggtagaggcg
      901 ggactgggga tttcggcgct tcctggtctg gcaatgcccc atgcgcccta ttcttcgatt
      961 attggtattc cactggtgga acccgttata cgtcggacat tggggattat tcgccgcaaa
     1021 gatgccgtgc tttctccggc agcagaacgc ttttttgcct tgctgattaa tttgtggact
     1081 gacgataaag acaatttgtg gaccaatatc gtcgaacgcc agagacatgc gttacaggag
     1141 ataggttaat acaccggcgc aggagagact gcgccgggta aatcagcggt tagtgtgcgt
     1201 taaccaccac ccacattggt ccctgcccga ccgcatagcg gcctttttca tgcagtagcc
     1261 cctgctcgcc aacaatttcg tataccgaga tgtggtgaga tttttgcccg gcggcaatca
     1321 gatacttgcc gctgtgatca acattgaagc cgcgcggctg ggtttccgtt ggctggaagc
     1381 cttctttact caacacgctg ccatcttccg aaacgctgaa aacggtaatc agactggcgg
     1441 tacggtcgca ggcgtataaa tgacgaccat ccggggtgat atgaatatca gccgcccaac
     1501 aggtgtcgga gaagttttcc ggcatcatat ccagcgtctg gacacattca atattgccgt
     1561 gtggatcttt cagttcccag acatccactg agctgtttaa ctcattgacg caatacgcat
     1621 attgttcgtt tggatggaat accatatgac gcgggccggc cccttcaacg gtagtcactt
     1681 ccgcagggtc ctgcgccacc agatgaccat catcgctgac cgtaaacagg caaatgcgat
     1741 cctgctttaa tgccggaacc cacagcgtac ggttgtccgg tgagatattg gcggaatggc
     1801 aaccgtccag cccctcgacc acatcgacga cgcccactgg caggccatct tccagacgcg
     1861 ttacgctcac gttacccgca ttgtaagaac ctacaaagac aaactgcccc tggtgatcgg
     1921 tggaaatatg cgtcggacta cccggcagcg cagactctgc ggcaaaggtc agtgcgccat
     1981 cgtccggggc aatacgatac gccaggacgc gaaactcagg gcgaacacca acatagagat
     2041 aacgtttgtc cgggctgacc accatcggct gcacctgccc cggcacatcg acaacctgtg
     2101 tcagcgtcag tgcgccttca tgattcaggt tccagacgtg aatttgctgg ctctcagggc
     2161 tggcgatata aactgtttgc ttcatgaatg ctcctttgca tttagcaact gactgtaaca
     2221 gctaaaatta gtcgcttttg gcggtgaatg ctgaattaaa acgcagaatt ttgtccgata
     2281 gtggtatcgg tgtaccattc gcgcagacga aaattcttaa atgacgcggt aaaagcgcgc
     2341 gccaacattg tgattggtga taacaccacc gacagcattg cccagttcat ttatagccac
     2401 ctgatttaat caggcggtta tcgacacact tttaatttgc gcgtacagcc acaggccagg
     2461 tttgatcgcc agttcatccc tggcccacgg gctgatacgc gcccacagcg ttttaccgcc
     2521 gacttccagc tccacttcca cctggccgtt gtcgtcataa ctattaacaa cttttgcccg
     2581 caatacgtta cgaatgctgg tttgctgcgg cggttgtaaa accagagaaa catcggaagc
     2641 ctgaatgcgg atacgtagcg cagcttgcag cggttcgtcc agcttattga cccacaaatg
     2701 ctgatcgccc agcgccagcg cggtcatcgc gtaatgcgga tgatgctcaa gtaccgtcac
     2761 tttcagaatg ctactttgtt gctctttcgg cagccacgga ttcatcacgc tactgcccca
     2821 cacttcctcc agcgcgccaa aggctttcac ctgaccgttt tccagtacca tcactctgtc
     2881 tgccagatgg aggatctcat ccagcgaatg gctgacatac aacatcggaa tgttgatttc
     2941 ccgtgtcagc cgttgcagat aaggcaacag ttcgcgttta cgcggaatat ccagtgacgc
     3001 cagcggttca tccagcaaca gcaattccgg tgctgtcagc aaagcccgac caatcgccac
     3061 gcgctgtttt tcgcctccgg acaggctgcc tggtaaacgg tcaagcaacg gttcaatgcc
     3121 taaaagcgcc accagcttat cgaactgatc gaccatactt ttcgacatgc cgtagcgcag
     3181 attgccacgc actttgtaat gcgggaacag ccgcgcatcc tgaaaaacat agccaacgcg
     3241 acgcttttcc ggcgtcaggc agataccttt ttcggcatca tttagtaccc gcccattgag
     3301 gacaatccgc cctttttgcg ggcgcgtcag tccactgatg gcgttaatca gcgaagtttt
     3361 tccggcaccg gagacgccaa agatagcagt gatgccattg gcgggcagtg tttcattaat
     3421 ggtcaggcaa tggttgccca acgtctggga aaaattcagc tccagcatga ttagcgcccc
     3481 gcccgttcac ggctgattct ggccagccat tctgaaatca acagggagat catcgccagc
     3541 gcaatagaaa taatgcacag tctcgccgcg ccactttcct cgccgggggt ctggatcagg
     3601 gtatacatgg cagagggaat ggttcgcgtt tcgccaggaa tgttcgacac aaaggtgatg
     3661 gttgcaccaa actcaccgag agaacgagca aaagccagta ccgtaccaac aataattccc
     3721 ggtaaggtca gcggtaacgt gatagtaaag aaaacgcgcc agcgcccggc acctagtgtt
     3781 cttgcggcct gttcgagttt gacatcaacc ccttccagcg ccagacgaat tgcccgcacc
     3841 atcagcggaa acgacatgac ggcggcagcg agaaccgcgc cgcgccagct aaaggcgaag
     3901 gtaataccaa accagtcata cagacgttca ccgataaatc cgcgccgtcc catcgaaact
     3961 aataataagt aaccgacgac cacgggcggt aacaccagcg gtagatgcag tacgctgtcg
     4021 agcagagctt tgcccggaaa cgtgcaacgc accagtaacc aggcaaaaaa gatcccaaac
     4081 ggcaggctaa acagcacagc cagggaagaa actttcaggc ttaataaaac tgcctgccat
     4141 tctggatcgg tcagtatcat tacttggttg taaatccgta acgtttaaag atttccgctg
     4201 cctgcggtcc cttcagataa tcataaaatg ctttcactgt cgcattgtta tgcccttcca
     4261 caacagcaac cggatattcc acttttttat gtgaatcttc cgggaaggtg gcaaccactt
     4321 ttaccccttt gctgacaact gcgtcagaac cgtaaacaat gcccaacggc gcttcattgc
     4381 gttcgaccaa cgccagcgcg ccacgaacat cttccgccgg agccagtttc ggggagagcg
     4441 tatcccatgc gcccagtttt tgcagtgctt cttttgcata aatgccagca gggacatgtt
     4501 ccggatcgcc aaccgcgagg cgaccgccat tcagcagcga agtccagtta gttgtgctgt
     4561 cgatgatgaa atctttctgc tcgctggctt tcggtgctac aacgaccagg ctattgccga
     4621 gcagtgtctg gcgcgaagcc gtatcgatcg cttttttatc aaccgcataa tccatccatt
     4681 tctgatcggc agagataaac agatccgcag gcgctcccgc ttcaatctga cgggccagag
     4741 tagatgacga agcgaaagaa gaaaccacat ccacgccttt ttctttttta tactgcgtag
     4801 caatgtcctg cattgcgtta gtcagtgatg cggcggcgaa caccgtgatt ttcccttcat
     4861 ctgccagtgt attgccagca acagcgaaag agagtgccgc cccggcaaac aagttcaacc
     4921 atttacgagc cattggtaac cccttaatgt aacgttatat aggtgacaat ataacggcta
     4981 gctcagaatt ttcccagcag ttattgctaa cctttgtgag gtagataaga aaaaatatcg
     5041 gctggaaaag caggaagttg agaaaaagaa atgcccgact aagcgggcat tcagggaatc
     5101 aatgattttg tccgggctgg tcttttttac caacaccaga aaagatgttg aatacttcac
     5161 caagaccgta aatcagaccc aggatgatgg ccatcacgac aggtaccatg attacggcga
     5221 ataccagact ttttaataac tctaacatgg tcaactccag atatagtcat gagactattc
     5281 taaccgctaa gcacagaaaa gcactcccct tttgtgcggt cagctttgcg tggcgttcgt
     5341 tttccgtcac aataagactt ttgccaggac attgttatgc aggccgaaat ccttctcacc
     5401 cttaagctcc aacaaaaatt attcgccgac ccgcgccgga tttcgctact aaaacacatt
     5461 gcgctttccg gttccattag ccagggagcg aaagatgccg gtatcagcta taaaagcgcc
     5521 tgggatgcca ttaacgagat gaatcagtta agcgagcata ttctggttga gcgcgcaaca
     5581 ggcggtaaag gtggcggcgg cgcagtactg acccgctatg gtcagcgact gattcagctc
     5641 tatgacttac tggcgcaaat ccagcaaaaa gcctttgatg tgttaagtga cgatgacgcc
     5701 ctgccgctga acagcctgct ggccgcgatc tcacgttttt cactgcaaac cagcgcccgt
     5761 aaccagtggt tcggtaccat caccgcccgc gatcatgatg acattcaaca gcatgttgat
     5821 gtcttactgg ctgacggaaa aacacgcctg aaagtcgcaa ttaccgcaca aagcggcgca
     5881 cgtctggggc tggatgaagg caaagaagtg ttgatattgc taaaagcgcc gtgggtaggc
     5941 attactcagg acgaagcggt cgcacaaaac gccgacaacc aattaccggg tattattagt
     6001 catattgagc gcggcgcaga gcagtgcgaa gtattaatgg cgctacccga cgggcaaaca
     6061 ctgtgcgcca cagtgccggt aaatgaagcg acttctcttc agcaagggca gaatgtcacg
     6121 gcctacttta atgccgacag cgtgattatc gccacgctgt gctaagcgtg ttgacaattt
     6181 gttatgaaac acgtatccct gtcagtaatc gctgcacaaa atgggatata aaatgtcatc
     6241 gttgcaaatt ttgcaaggca cgtttcgtct tagcgacaca aaaacgctgc aattgcctca
     6301 gctaacgtta aacgcgggtg atagttgggc gtttgtcggt tcgaatggaa gcgggaaatc
     6361 ggccctggcc cgcgcgctgg cgggggaact tccgcttttg aaaggtgaac ggcaaagcca
     6421 gttttcccac atcactcgtc tctccttcga gcaattgcaa aaactcgtca gcgacgaatg
     6481 gcagcggaat aacaccgata tgctcggccc tggcgaagat gacaccggac gcactacggc
     6541 tgaaatcatt caggatgaag taaaggatgc accgcgttgc atgcaactgg cgcagcagtt
     6601 cggtattacc gccctcctcg accgacgctt taaatacctt tccactggcg agacgcgaaa
     6661 aaccctgctg tgtcaggcgc tgatgtcgga gcctgacttg ttgattcttg atgagccgtt
     6721 cgatggcctg gatgttgcct cacgtcagca gctggctgag cgactcgcct cgttacatca
     6781 gtccggtatt actctggtac tggtgctcaa tcgcttcgat gagatcccgg agtttgtcca
     6841 gtttgctggc gtgctggcgg attgcacgtt agcggaaact ggcgctaaag aggaactgct
     6901 ccaacaagca ctcgtcgcgc aactggcgca tagtgaacag cttgaaggtg tgcaactgcc
     6961 ggagccggat gaaccttcag cacgtcacgc cttacccgcc aacgaaccgc gcattgtgct
     7021 gaacaatggc gtggtttctt ataacgatcg ccccattctt aataacctta gctggcaggt
     7081 gaatccaggt gaacactggc aaattgtcgg gccaaatggt gcaggaaaat cgacgttatt
     7141 aaacctggtt actggcgatc atccgcaagg ttacagcaac gatttgacgc ttttcggacg
     7201 acgtcgcggc agcggcgaaa ccatctggga tatcaaaaag catatcggtt acgtcagcag
     7261 tagtttgcat ctggattacc gggtcagcac taccgtgcgt aatgtgattc tttctggcta
     7321 ttttgattcg attggcattt atcaggccgt ttcggatcgc cagcaaaaac tggtgcagca
     7381 gtggctggat attctcggca ttgataaacg tacggctgac gctccgttcc atagtctttc
     7441 ctggggacag cagcgtctgg cgctgatcgt ccgcgcactg gtgaaacatc cgacgttgct
     7501 tattctcgat gaaccactac aggggcttga tccgctcaat cgccagctta tccgccgttt
     7561 tgttgatgtg ctgattagcg aaggtgaaac gcaattgtta tttgtttcgc accacgctga
     7621 agatgcgcct gcctgtatta cccatcgcct tgagttcgtg ccggacggtg gactctatcg
     7681 ctatgtgctg acaaaaatat attgagtcgg tagtgctgac cttgccggag gcggcctaag
     7741 cgccctctcc ggccaacggt tcgacgcatg caggcatgaa accgcttctt ttttcagata
     7801 aaaagcgcaa tcattcataa accctctgtt ttataatcac ttaatcgcgc ataaaaaacg
     7861 gctaaattct tgtgtaaacg attccactaa tttattccat gtcacacttt tcgcatcttt
     7921 gttatgctat ggttatttca taccataagc ctaatggagc gaattatgag agttctggtt
     7981 accggtggta gcggttacat tggaagtcat acctgcgtgc aattactgca aaacggtcat
     8041 gatgtcatca ttcttgataa cctctgtaac agtaagcgca gcgtactgcc tgttatcgag
     8101 cgcttaggcg gcaaacatcc aacgtttgtt gaaggcgata ttcgtaacga agcgttgatg
     8161 accgagatac tgcacgatca cgctatcgac accgtgatcc acttcgccgg gctgaaagcc
     8221 gtcggcgaat cggtacaaaa accgctggaa tattacgaca acaatgtcaa cggcactctg
     8281 cgcctgatta gcgccatgcg cgccgctaac gtcaaaaact ttatttttag ctcctccgcc
     8341 accgtttatg gcgatcagcc caaaattcca tacgttgaaa gcttcccgac cggcacaccg
     8401 caaagccctt atggcaaaag caaattgatg gtggaacaga tcctcaccga cctgcaaaaa
     8461 gcccagccgg actggagcat tgccctgctg cgctacttca acccggttgg cgcgcatccg
     8521 tcgggcgata tgggcgaaga tccgcaaggc attccgaata acctgatgcc atacatcgcc
     8581 caggttgctg taggccgtcg cgactcgctg gcgatttttg gtaacgatta tccgaccgaa
     8641 gatggtactg gcgtacgcga ttacatccac gtaatggatc tggcggacgg tcacgtcgtg
     8701 gcgatggaaa aactggcgaa caagccaggc gtacacatct acaacctcgg tgctggcgta
     8761 ggcagcagcg tgctggacgt ggttaatgcc ttcagcaaag cctgcggcaa accggttaac
     8821 tatcattttg caccgcgtcg cgaaggcgac cttccggcct actgggcgga cgccagcaaa
     8881 gccgaccgtg aactgaactg gcgcgtaacg cgcacactcg atgaaatggc gcaggacacc
     8941 tggcactggc agtcacgcca tccacaggga tatcccgatt aaggaacgac catgacgcaa
     9001 tttaatcccg ttgatcatcc acatcgccgc tacaacccgc tcaccgggca atggattctg
     9061 gtttcaccgc accgcgctaa gcgcccctgg cagggggcgc aggaaacgcc agccaaacag
     9121 gtgttacctg cgcacgatcc agattgcttc ctctgcgcag gtaatgtgcg ggtgacaggc
     9181 gataaaaacc ccgattacac cgggacttac gttttcacta atgactttgc ggccttgatg
     9241 tctgacacgc cagatgcgcc agaaagcaac gatcctttga tgcgttgcca gagcgcgcgc
     9301 ggtaccagcc gggtgatctg cttttcaccg gatcacagta aaacgttgcc agaactgagc
     9361 gttgcggcat tgacggaaat cgtcaaaacc tggcaggagc aaaccgcaga gctgggaaaa
     9421 acgtacccgt gggtgcaggt ctttgaaaac aaaggtgcgg cgatgggctg ctctaacccg
     9481 catccgcacg gacagatttg ggcaaatagc ttcctgccta acgaagctga gcgcgaagac
     9541 cgcctgcaaa aagaatattt tgccgaacag aaatcaccaa tgctggtgga ttatgttcag
     9601 cgcgagctgg cagacggtag ccgtaccgtt gtcgaaaccg aacactggtt agccgttgtg
     9661 ccttactggg ctgcctggcc gttcgaaacg ctactgctgc ccaaagccca cgttttgcgg
     9721 atcaccgatt tgaccgacgc ccagcgcagc gatttggcgc tggcgttgaa aaagctgacc
     9781 agtcgttatg acaacctctt ccagtgctcc ttcccctact ctatgggctg gcacggcgca
     9841 ccatttaatg gcgaagagaa tcaacactgg cagctgcacg cgcactttta tccgcctctg
     9901 ctgcgctccg ccaccgtacg taaatttatg gttggttatg aaatgctggc agagacccag
     9961 cgagacctga ccgcagaaca ggcagcagag cgtttgcgcg cagtcagcga tatccatttt
    10021 cgcgaatccg gagtgtaaga aatgagtctg aaagaaaaaa cacaatctct gtttgccaac
    10081 gcatttggct accctgccac tcacaccatt caggcgcctg gccgcgtgaa tttgattggt
    10141 gaacacaccg actacaacga cggtttcgtt ctgccctgcg cgattgatta tcaaaccgtg
    10201 atcagctgtg caccacgcga tgaccgtaaa gttcgcgtga tggcagccga ttatgaaaat
    10261 cagctcgacg agttttccct caatgcgccc attgtcgcgc atgaaaacta tcaatgggcg
    10321 aactacgttc gtggcgtggt gaaacatctg caactgcgta acaacagctt cggcggtgtg
    10381 gacatggtga tcagcggcaa tgtgccgcag ggtgccgggt taagttcttc cgcttcactg
    10441 gaagtcgcgg tcggaaccgt attgcagcag ctttatcatc tgccgctgga cggcgcacaa
    10501 atcgcgctta acggtcagga agcagaaaac cagtttgttg gctgtaactg cgggatcatg
    10561 gatcagctaa tttccgcact cggcaagaaa gatcatgcct tgctgattga ctgccgctca
    10621 ctggggacta aagcagtttc catgccgaaa ggtgtggctg tcgtcatcat caacagtaac
    10681 ttcaaacgta ccctggttgg cagcgaatac aacacccgtc gtgaacagtg cgaaaccggt
    10741 gcgcgtttct tccagcagcc agctctgcgc gatgtcacca ttgaagagtt caacgctgtt
    10801 gcacatgagc tggacccaat cgtggcgaaa cgcgtgcgtc atatcctgac tgaaaacgcc
    10861 cgcaccgttg aagctgccag cgcgctggag caaggcgatc tgaaacgtat gggcgagttg
    10921 atggcggagt ctcatgcctc tatgcgcgat gatttcgaaa tcaccgtgcc gcaaattgac
    10981 actctggtag aaatcgtcaa agcagtgatt ggcgacaaag gtggcgtacg catgaccggc
    11041 ggcggatttg gcggctgtat cgtcgcgctg atcccggaag agctggtgcc tgccgtacag
    11101 caagctgtcg ctgaacaata tgaagcaaaa acaggtatta aagagacttt ttacgtttgt
    11161 aaaccatcac aaggagcagg acagtgctga acgaaactcc cgcactggca cccgatggtc
    11221 agccgtaccg actgttaact ttgcgtaaca acgcagggat ggtagtcacg ctgatggact
    11281 ggggtgcgac tttactttca gctcgtattc cgctttccga tggcagcgtc cgcgaggcgc
    11341 tgctcggctg tgccagcccg gaatgctatc aggatcaggc cgcgtttctg ggggcctcta
    11401 ttggtcgtta tgccaaccgc atcgccaata gccgttatac ctttgacggt gaaacggtga
    11461 cgctttcgcc aagtcagggc gttaaccagc tgcacggcgg cccggaaggg ttcgacaaac
    11521 gtcgctggca gattgtgaac cagaacgatc gtcaggtgct gtttgccctg agttcagatg
    11581 atggcgatca gggcttcccg ggtaatctcg gcgcgacggt gcaatatcgt ctgaccgacg
    11641 ataaccgtat ctccattact tatcgcgcca cagttgataa atcttgcccg gtgaatatga
    11701 ctaatcacgt ctatttcaat cttgacggcg agcagtctga cgtgcgcaat cacaagttgc
    11761 agattctggc gggcgaatat ctgccggttg atgaaggcgg cattccgcac gacggcctga
    11821 aatctgtcgc cggaacctct tttgatttcc gcagcgccaa aatcatcgcc agtgagtttc
    11881 ttgccgacga cgatcagcgc aaagtgaaag gttacgatca cgcattcttg ttacaggcca
    11941 aaggcgatgg caagaaagtg gcggcgcatg tctggtcagc agatgaaaaa ttgcagctga
    12001 aggtctacac caccgctccc gctcttcaat tctactccgg caacttcctc ggcggcacac
    12061 cgtcgcgggg aaccgaacct tacgccgact ggcaaggact ggcgttggaa agcgagtttc
    12121 tgccggacag cccgaaccac cctgaatggc cgcaaccgga ctgcttcctg cgtcctggcg
    12181 aagagtattc cagcctgacg gaatatcagt ttattgctga gtaatgttaa gccctcgtca
    12241 tgagggcttt atcttcatat tgttcaaatc accagcaaac accgacatat ttgcaactca
    12301 atattcacaa caaccttaca ctgcgccact attttcgcta tggttatgcg taagcattgc
    12361 tgttgcttcg tcacggcaat ataatgagaa ttattatcat aaaaagataa tttgaggagt
    12421 aagtatatgg ctgtaactaa gctggttctg gttcgtcatg gcgaaagtca gtggaacaaa
    12481 gaaaaccgtt tcaccggttg gtacgacgtg gatctgtctg agaaaggcgt aagcgaagca
    12541 aaagcagcag gtaagctgct gaaagaggaa ggttacagct ttgactttgc ttacacttct
    12601 gtgctgaaac gcgctatcca taccctgtgg aatgtgctgg acgaactgga tcaggcatgg
    12661 ctgcccgttg agaaatcctg gaaactgaac gaacgtcact acggtgcgtt gcagggtctg
    12721 aacaaagcgg aaaccgccga aaagtatggc gacgagcagg tgaaacaatg gcgtcgtggt
    12781 tttgccgtga ctccgccgga actgactaaa gatgatgagc gttatccggg tcacgatccg
    12841 cgttacgcga aactgagcga gaaagagctg ccgctgactg aaagcctggc gctgaccatt
    12901 gaccgcgtga tcccttactg gaatgaaact attctaccgc gtatgaagag cggtgagcgc
    12961 gtgatcatcg ctgcacacgg taactcttta cgtgcgctgg tgaaatatct tgataacatg
    13021 agtgaagaag agattcttga gctgaatatc ccgaccggcg tgccgctggt gtatgagttc
    13081 gacgagaatt tcaaaccgct gaaacgctat tatctgggta atgctgacga gatcgcagcg
    13141 aaagcagcgg cggttgctaa ccagggtaaa gcgaagtaaa cgtcattcgt ttaaaaatga
    13201 gaaagccgac tgcaagtgag tcggcttttt tgttgctaac aatggagcac attgcctgat
    13261 gcgacgctgc gcgtcttatc aggcctgtgg tgattcatcg gatacgccac tctgacggcg
    13321 catccgacaa ttaaacctta cccgcgacgc gcttttactg cattcgccag ttgacgtaac
    13381 agagcatcgg tatcttccca gccaatgcag gcatcggtga tgctcttacc gtaggccagc
    13441 ggctccccgc tatcgaggct ctgattgcct tccaccagat ggctttccac catcacgcca
    13501 ataatggcct tttcgccacc ggcaatctgc tggcaaacgt cagcacaaac atccatctgc
    13561 tttttgaatt gtttggacga gttagcatgg ctgaaatcga tcatcacctg tgctggcagg
    13621 cctgctttgt tcagcccttc tttcacttca gcaacgtgct tcgcgctgta gttaggctct
    13681 ttaccgccgc gcagaatgat atggcaatca ccgttaccgc tggtattcac aatcgccgaa
    13741 tgcccccatt tagttacgga caggaagcag tgcggcgcac cggcggcatt aatggcatcg
    13801 atagccactt taatcgtacc gtctgtgcca tttttaaaac ctaccggaca agaaagacca
    13861 gacgccagtt cgcggtgcac ctgcgattcc gtggtacgtg caccaattgc gccccagctc
    13921 atcaggtcag cgagatactg aggagtgatc atatcgagaa actcacccgc cgctggcaga
    13981 ccgctgtcgt taatatcaag cagcaattta cgggctatac gcagaccgtc gttgatctgg
    14041 aagctgttat ccatatgcgg atcgttaatc aaccctttcc agcccaccgt ggtacgcggc
    14101 ttttcaaaat agacgcgcat tacgatttcc agctcatctt tcagctcttc acgcagcgcc
    14161 agcaagcgag tggcatactc tttagccgcg acaggatcat gaattgagca cgggccaatc
    14221 accaccaaca ggcgagcatc attacctttc aggatcttat ggatcgcttt tcgggcatgg
    14281 gcaaccgtat tcgcggcatt ttcagttacg gggaattttt ccagcaatgc gacaggagga
    14341 agtaactctt tgatttcttt gatgcgtaaa tcgtcgttct gataattcat gtctgttcca
    14401 gtgttgccat acttatctta gtgaatgcaa tacttccaat ctatatcttc cgtcagaatg
    14461 tgtaaacggg gttttacact atgaacggat taatcctgga attagcaaga aaaacgccag
    14521 attgtcgcga aaaacgagat ctctcctaca atttctaact gtaactcctt tgtttgttaa
    14581 ttatttcaag attctctgct gcgtttcata aactggctga aatcttaaac caatgcctta
    14641 tattcacctg caaatgcact gttggaagag gttatccgac ataacgacca taacaggagc
    14701 atcctatgaa aatgacaaaa ctggccacat tatttctgac tgccactcta agccttgcca
    14761 gcggtgccgc actggctgcc gatagcggag cgcaaactaa taacggccag gcaaacgccg
    14821 cagctgatgc gggccaggta gcccctgacg cccgtgaaaa tgtcgcgcca aataacgtcg
    14881 acaataacgg ggtaaatacc ggttctggcg gcacaatgct gcattcggat ggttcttcaa
    14941 tgaacaatga cggaatgacc aaagatgaag agcacaagaa caccatgtgc aaagatggtc
    15001 gatgtccgga tatcaataaa aaggtacaaa tcggtgatgg catcaataat gatgtcgata
    15061 ccaaaaccga cggtaccacg cagtaaccct gcaaaatagc caataatggg agagcggagt
    15121 ctctcccttt taatttttca cttctaaaag cagtatgatg tcataaagtt aagtgagaat
    15181 aattaaggga tgactcgtca tggcgcactc acactcacac acgtcctcac acctgccaga
    15241 agataataat gctcgtcgct tgttgtatgc tttcggcgtg actgccgggt ttatgctggt
    15301 agaagtcgtt ggtggttttc tttctggttc tctggcattg ctggccgatg cgggtcatat
    15361 gttgaccgat actgccgccc tgcttttcgc cctactcgcc gtgcaatttt cacgtcgtcc
    15421 tcccaccatt cgccacactt tcggttggct aagactgacc acgctcgcgg cgtttgtgaa
    15481 tgccatcgcc ctcgtggtaa ttaccatttt gattgtctgg gaggcgatag aacgcttccg
    15541 cacgccgcgt ccggtcgagg gcggcatgat gatggcaatt gccgtggctg ggttgctggc
    15601 aaatatactt tctttctggt tacttcatca cggcagcgaa gagaaaaacc tcaacgtacg
    15661 agcagcggca ttgcatgtgc tcggggacct gttgggttcg gttggggcta ttattgccgc
    15721 gttgattatt atctggaccg gctggacacc tgctgacccc attctctcga tagtggtgtc
    15781 gcttctggtt ctgcgtagcg cgtggcgact gttgaaagat agcgtgaatg aattacttga
    15841 aggtgcaccg gtatcgctgg atatcgctga actgaagcgt cgtatgtgcc gggaaatccc
    15901 ggaagtccgc aatgtgcacc atgtacatgt atggatggta ggcgagaagc cggtgataac
    15961 gctgcatgtg caggtgatcc cgccacacga tcacgatgcc ttgttggatc agatccaaca
    16021 ctacctgatg gatcactatc agattgagca cgccaccatt cagatggaat atcaaccttg
    16081 tcatgggcca gactgccacc tgaatgaagg ggtgtctggc cattcacatc atcaccatta
    16141 atgggacagc gcgcgtgagc ctctttcacg tgcgctgttg atccacatcc gactgccgtt
    16201 gagcgcaata aaggtcagga tgatgtactc cagagacatg gcgtaaacgc cctgaagtgc
    16261 aaaaataacg acgctaatca cgttaataat cacccacaac agccagtttt ccacatactt
    16321 acgcgtcatc agaatcattg ccacgataga taacaccatc atgcatgaat cccagaacgg
    16381 gaaagcgtcc ggttgcagtt caggcatcac aacctgtaat cctaatgctt gcatgatcat
    16441 gactgccacg cgggtcaaaa atgcaaacac cggattgata aataccgtca tcagaccaat
    16501 cgaaacaacg caaaccgcca accagctgag cgccttcggc aatggtaacc agcgaatttt
    16561 caactccgcc tcgttctgac tggtttgtcg cgaccacgca taccaaccgt aaatattcgc
    16621 ggcaaagaaa aacacctgta atagcaggct ggcatacagc tgaatctgaa agaaaataat
    16681 gccaaacaag gtgacgttga tcaggccaaa gaagtagttg ctgatcttct ccagactggc
    16741 aaggccaata cacagcaacc cggcgatcgt gcctaccgct tcgatccatg agagatcata
    16801 accgcctgcc cctattggta tatgtaccag gatattctgc acactaaaaa aatccatctt
    16861 tcccccaaag cgtaacgtta cgccttatta ttcgttatcc acgtagtgta gccgcaaaat
    16921 ccagcatacg attgagcggc accagcgccc tctctcgcag cctttcatca agaacctcgt
    16981 gattgcttcc ttcctgttct aatgcctctg cgatggactg aaggccattc atggccatcc
    17041 acggacaatg cgcgcag
//
LOCUS       DAKISA010000096        16772 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.096, whole genome shotgun sequence.
ACCESSION   DAKISA010000096 DAKISA010000000
VERSION     DAKISA010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16772
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.096"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            78..2411
                     /gene="bisC"
                     /locus_tag="OVW45_003239"
     CDS             78..2411
                     /gene="bisC"
                     /locus_tag="OVW45_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCV5527615.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRQLAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(2380..2820)
                     /locus_tag="OVW45_003240"
     CDS             complement(2380..2820)
                     /locus_tag="OVW45_003240"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCV5527616.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(2817..3380)
                     /gene="tag"
                     /locus_tag="OVW45_003241"
     CDS             complement(2817..3380)
                     /gene="tag"
                     /locus_tag="OVW45_003241"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCV5527617.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            3538..4236
                     /locus_tag="OVW45_003242"
     CDS             3538..4236
                     /locus_tag="OVW45_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCV5527618.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPNYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTQTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            4465..5673
                     /locus_tag="OVW45_003243"
     CDS             4465..5673
                     /locus_tag="OVW45_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCV5527619.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            5997..7688
                     /gene="eptB"
                     /locus_tag="OVW45_003244"
     CDS             5997..7688
                     /gene="eptB"
                     /locus_tag="OVW45_003244"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCV5527620.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
     gene            7780..7856
                     /locus_tag="OVW45_003245"
     tRNA            7780..7856
                     /locus_tag="OVW45_003245"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7814..7816,aa:Pro,seq:cgg)
     gene            7891..8024
                     /locus_tag="OVW45_003246"
     ncRNA           7891..8024
                     /ncRNA_class="other"
                     /locus_tag="OVW45_003246"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            8769..10376
                     /gene="dppA"
                     /locus_tag="OVW45_003247"
     CDS             8769..10376
                     /gene="dppA"
                     /locus_tag="OVW45_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCV5527621.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQTPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10684..11703
                     /gene="dppB"
                     /locus_tag="OVW45_003248"
     CDS             10684..11703
                     /gene="dppB"
                     /locus_tag="OVW45_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCV5527622.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11713..12615
                     /gene="dppC"
                     /locus_tag="OVW45_003249"
     CDS             11713..12615
                     /gene="dppC"
                     /locus_tag="OVW45_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCV5527623.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYIVIVLF
                     ITIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12626..13609
                     /gene="dppD"
                     /locus_tag="OVW45_003250"
     CDS             12626..13609
                     /gene="dppD"
                     /locus_tag="OVW45_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCV5527624.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13606..14610
                     /gene="dppF"
                     /locus_tag="OVW45_003251"
     CDS             13606..14610
                     /gene="dppF"
                     /locus_tag="OVW45_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCV5527625.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14640..15911)
                     /locus_tag="OVW45_003252"
     CDS             complement(14640..15911)
                     /locus_tag="OVW45_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCV5527626.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            16387..16494
                     /locus_tag="OVW45_003253"
     CDS             16387..16494
                     /locus_tag="OVW45_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCV5527627.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT         3989 a         4177 c         4530 g         4076 t
ORIGIN      
        1 ttattcctgg gaatactcgg aaatttgtaa gtaatattta actgctcaat acatctaacc
       61 tttcaggagt cttcggtttg gccaactcat cctcacgata ttccgttctg actgccgccc
      121 actgggggcc catgctggtt gaaaccgacg gcgaaaccgt gtttagctcg cgtggcgcgt
      181 tagccacagg aatggaaaac tccttgcaga gcgcggttcg cgaccaggtt cacagcaata
      241 cgcgggtacg atttccaatg gtgcgcaaag gctttctcgc gtcaccggaa aacccgcaag
      301 gcattcgtgg gcaggatgaa tttgttcgcg tgagttggga tgaggcgctg gatcttattc
      361 accaacaaca taaacgcatt cgtgaggctt atggtccggc atcgattttt gctggttcct
      421 acggctggcg ttcaaacggc gtgctgcata aggcctcgac attattacaa cgctatatgg
      481 cgctggcagg cggttatacc gggcatctgg gggattattc gaccggtgcg gcacaggcga
      541 tcatgccgta tgtcgtaggt ggcagcgaag tttatcaaca gcaaaccagt tggccgctgg
      601 tgctggaaca tagcgatgtc gtggtgttgt ggagtgctaa cccactcaat acgctgaaaa
      661 ttgcgtggaa tgcatccgat gagcaggggc tttcttactt ttccgcactg cgtgacagtg
      721 ggaaaaagct gatctgcatt gatccaatgc gatcggaaac cgtcgatttc tttggcgata
      781 aaatggagtg ggtggcaccg cacatgggca ccgatgttgc gctgatgctg gggatcgccc
      841 atacgctggt ggaaaatggt tggcacgacg aagcgtttct ggcgcgttgc accacaggtt
      901 atgccgtctt cgcctcttat ttgctgggcg agagtgacgg aatagcgaaa accgccgaat
      961 gggcagcaga gatttgtggt gttggcgcag cgaaaatccg ccagctggcg gctattttcc
     1021 accaaaatac caccatgctg atggcaggct ggggaatgca gcgccaacag tttggtgagc
     1081 aaaaacactg gatgatcgtc acgctggcag caatgttggg gcaaatcggc acacccggcg
     1141 gcggttttgg tctttcttac cattttgcca atggtggtaa ccccacgcgg cgttctgcgg
     1201 tgctctcttc catgcagggc agcttgccgg gtggctgcga tgcggtggat aaaatccctg
     1261 ttgcccgcat tgttgaagca ctggaaaacc ctggtggcgc atatcaacac aacggtatga
     1321 accgacattt cccggatatt cgttttatct ggtgggcggg cggtgccaac tttactcatc
     1381 atcaggatac caatcgtctg atccgtgcct ggcaaaaacc ggagctggtg gtgatctctg
     1441 aatgcttctg gacggcggcg gcgaaacacg cggatatcgt tctgcctgcg actacctctt
     1501 ttgagcgtaa tgatctcacc atgaccggtg attacagtaa tcagcatctg gtgccgatga
     1561 agcaagtggt gccgccacgc tatgaagcgc gtaatgattt tgatgttttt gccgagttga
     1621 gtgaacgttg ggagaagggc ggctatgcgc ggtttacgga aggaaaaagt cagctgcaat
     1681 ggctggaaac gttttataac gttgcccggc agcgcggggc aagccagcag gttgaattgc
     1741 cgccatttgc tgagttctgg caagccaacc agttaattga gatgccggaa aacccggaca
     1801 gcgagcggtt tattcgcttc gctgattttt gccgcgatcc gctggcgcat ccgttaaaaa
     1861 ccgccagcgg caagattgaa atcttctcac agcgtattgc cgattacggt tacccggatt
     1921 gccctgggca tccaatgtgg ctggagccgg acgaatggca gggcaatgcc gaaccagaac
     1981 agttgcaggt actttctgcc catccggcgc accgcctgca cagccagctg aattacagtt
     2041 ctctgcgcga attgtacgcg gtagcaaacc gtgagcctgt caccattcat cctgacgatg
     2101 ctcaggcgcg cggcataacc gaaggggata tggtgcgggt gtggaactcc cgtgggcaga
     2161 tccttgccgg agcggtcatt agcgagggaa ttaaacctgg cgtgatttgc attcacgaag
     2221 gggcatggcc ggatctggat ttaaccgctg acggtatttg taaaaacggc gcagtgaacg
     2281 tgctgaccaa agatctcccc agctcgcggc tggggaatgg ctgtgcgggt aatacggcgc
     2341 tggcatggct ggaaaaatac aacggtccgg aactgacact tacagcgttt gaaccaccgg
     2401 ccagctcata atccaggtgg gtagttgggt ttcatcctgc catgcgcaat cgacaatgtg
     2461 aaaaccctgc gcacggtaaa aatctatcgc cggttgattt ttttgataaa cctccagcat
     2521 cagatgggga tagcgctgct gcacatactg catcagcgcc ttaccaatac cgcgtctgac
     2581 ggccttcggt gcgacaaaca ttgctgccag aaatcggcct tccataatgc tgacaaaacc
     2641 gagaagctta ccgtcctctt cccagaccca gttttgcgcg ttggcaagat aggcatcccg
     2701 caccagcgga atgcagtcac gccagtaatt cgcttttata aagggatgcc cccaggttgt
     2761 actttccagc cacagttcga ggatcgcggg gagttctgaa cgttgcgctt cccgaatcat
     2821 ggtttatttc ccggatagca acagcagcca accacatgat cattcaccag cccacatgcc
     2881 tgcataaagg agtaacagat tgtggtgccg acaaacttaa aaccacgttt tttcagtgct
     2941 ttagataggg cgtcggaggc ggacgtagat gtgggaattt cgctcaacgt tgtggcttgt
     3001 gtcacctgtg gctgatgatt tacaaacgac cagacaaagt cggcaaacgg ttcgccgttc
     3061 tgttccattt gcaggtacgc ccgcgcatta ccaataattg cctgaatttt ccctcgatgg
     3121 cggataatcc cggcgtcctg taccagtctt tcgacatcct cttcctgcat tgctgcgacc
     3181 ttcaccggat cgaactgatg aaagcaggcg cgatagtttt cgcgtttttt gaggacggtg
     3241 atccacgata acccagcctg ctgcccttca aggcagatca tttcgaacag ttttttactg
     3301 tcagtttcag gcacgcccca ttcattatca tggtaggcaa tataaagcgg gtcctgactc
     3361 acccagccgc aacgttccat actttccctc gctatgtacc cgatcgtaaa aatttcagcc
     3421 gcttttcttg acgcatcggt gaaaaagtca atatttaata cagggctact atgcccgata
     3481 tccttcatac aatgacaata atattgccga attcggctct tccctggagt cgtgttaatg
     3541 atcataaaaa aaagcggtgg tcgctggcag ctaagcctgc tggcgagcgt ggtaatcagt
     3601 gccttttttc tcaacacagc ttacgcctgg caacaagaat atatcgttga tacgcaaccc
     3661 ggacattcca cagagcgtta cacctgggat agtgatcatc aacctaatta caacgatatt
     3721 ttgtcgcaac gtattcaaag tagccaaagg gcgctgggac tggaagtcaa tctggcggaa
     3781 gaaactcctg tggatgtgac cagcagtatg agtatgggct ggaattttcc tttatatgaa
     3841 caggttacaa ccggcccggt cgcggcatta cattacgatg gcacaaccac ctcgatgtat
     3901 aacgagtttg gcgacagtac taccacgcag accgatccgt tatggcatgc cagcgtgagt
     3961 accttaggct ggcgtgttga ctcccggctt ggcgatctcc gaccctgggc gcaaatcagc
     4021 tataaccagc aatttggcga gaatatctgg aaggcgcaat caggcctgag ccggatgacg
     4081 gcgacaaacc agaacggcaa ctggctggat gtcaccgtag gcgctgatat gttgctcaat
     4141 caaaatattg ccgcctatgc cgcgctaact caggcagaaa ataccactaa taatagcgac
     4201 tatctgtata cgatgggggt tagcgccaga ttttaacgta acagtcacaa ttgaaaccat
     4261 tgaataacaa taattgtggc gatagtgggt gctaacttac caaataataa atttggtgaa
     4321 taattgtcgc gtcattcatt cctgaactaa ggcatttcat tccgttctga tggcatttca
     4381 tgccgttttt ccccaggcat aaagtgcact tcgttatggt tgtcggcaga gatttttcct
     4441 ttttattact gcaggaatac tgccatgaca ccttcaaatt atcagcgtac ccgctggctg
     4501 acactcatcg gtactatcat tacccagttt gcgctggggt cggtttatac ctggagcctg
     4561 tttaatggcg cgctttccgc caagctggat gcgccggtaa gccaggtcgc tttctctttc
     4621 ggcttgttaa gtctggggct ggcaatttcg tcttctgttg cgggcaaatt acaggaacgt
     4681 tttggcgtta aacgcgtcac catggcttcc ggcattttgc tgggattagg cttcttcctg
     4741 accgcgcatt ccaacaacct gatgatgctg tggttaagcg ccggtgtgct ggtcggtctg
     4801 gcggatggcg cgggttacct gctgacgctc tctaactgcg tgaagtggtt cccggagcgt
     4861 aaaggtctga tctccgcgtt cgctatcggt tcttatggtc tgggtagcct gggtttcaaa
     4921 tttatcgaca cgcagctgct cgaaacggtg ggtctggaaa aaacatttgt gatttgggga
     4981 gcgattgcgc tggtgatgat tgttttcggc gcaacgttaa tgaaagacgc accaaaacag
     5041 gaagtgaaaa ccagcaatgg tgtggtggag aaagattaca cgctggcaga gtcgatgcgt
     5101 aaaccgcagt actggatgtt agcggtaatg ttcctgactg cctgcatgag cggcctgtac
     5161 gtgattgggg tagcgaaaga tatcgcccaa agtctggcac accttgatgt ggtttccgca
     5221 gccaatgcag taacggttat ttccatcgct aacctttcag gtcgtctggt gctgggcatt
     5281 ctgtctgaca aaattgcccg tatccgtgtt atcaccattg gtcaggtgat ttcgctggtg
     5341 ggtatggcgg ccctgctgtt tgcaccatta aatgcagtga cgttctttgc agcgattgcc
     5401 tgcgtggcat ttaactttgg cggcactatt accgtctttc catcactggt cagtgagttc
     5461 ttcggcctca ataacctggc gaaaaactac ggtgtgattt acctcggttt cggtatcggt
     5521 agcatttttg gttcgattat cgcctcactg tttggcggtt tctatgtgac cttctacgtg
     5581 attttcgccc tgctgattct gtcattggcg ctttctacga cgattcgtca gccagagcag
     5641 aaaatgttgc gtgaggcgca tggctccctt taatgctcag ccgcgagcta ctgggcttca
     5701 gcttccgtgt gcatcaaacg catcaacgaa atgtataaac aatgactcgt aaaatttggg
     5761 ccagttaact ggcccttttt actgcttttt gtaacgctaa agtctctttt caaacttgca
     5821 tttttgtaaa tttgtgcttc atgcacactc tttccccaca ctttttcgct ttgctgtggt
     5881 ctacttattc gcgcgtgtag attttactta tctgactacc tccgcacttt tcccctgccg
     5941 ggcctgaaaa gccgttaagc agggtgttat cacctgtttg tccagggttt gtttgcatga
     6001 gatacatcaa atcgattaca cagcagaagc tgagcttttt gcttgcaatc tatattggcc
     6061 tttttatgaa tggcgcggtt ttttatcgcc gcttcggcag ctatgcgcac gattttaccg
     6121 tctggaaagg cgtttctgct gttgttgaac tggccgccac cgtactggtg accttctttt
     6181 tactacgtct tctatcgctg tttggccgcc gcagctggcg tattctggcg tcgctggtgg
     6241 tgctcttttc cgcaggtgcc agctattaca tgaccttcct taatgtggtc attggttatg
     6301 gcatcatcgc ttccgtcatg accaccgata tcgacctgtc aaaagaagtt gttggtctga
     6361 actttattct ctggttaatc gccgttagtg cattgcctct tatccttatc tggaataacc
     6421 gctgtcgcta caccttgctc cgacaactgc gaaccccagg gcagcgtatt cgcagcctgg
     6481 cggtcgtcgt actggcgggt attatggttt gggcaccgat tcgtttgctg gatatccagc
     6541 agaagaaagt ggagagggcg accggcgttg atttgccgag ttatggcggt gtcgtagcga
     6601 actcttatct gccatcaaac tggctttctg cgttggggct gtatgcctgg gcgcgggtcg
     6661 atgaatcttc cgataataat tcattgctta atccggcgaa gaagtttaca taccaggctc
     6721 cacaaaacgt tgatgatact tatgtcgtgt ttatcatcgg tgaaaccacg cgttgggacc
     6781 atatgggtat tttcggctat gagcgtaata ccacgccgaa actggcccag gagaaaaatc
     6841 tggcggcgtt ccgtggttac tcctgtgata ccgcaaccaa actctcactg cgttgcatgt
     6901 ttgtacgtca ggggggcgcg gaagataatc cgcagcgcac attaaaagaa cagaacattt
     6961 tcgcggttct gaagcagtta ggattcagtt ctgacctcta cgctatgcag agcgaaatgt
     7021 ggttctacag caacacgatg gcggacaaca ttgcttatcg tgagcagatt ggtgcggagc
     7081 cacgtaatcg tggcaagccg gtagatgata tgttgctggt agacgaaatg cagcaatcgc
     7141 tagggcgcaa cccggatggt aagcatctga tcattctgca taccaaaggt tcgcatttta
     7201 actacaccca gcgttatccg cgtagcttcg cgcagtggaa gccggaatgt attggtgttg
     7261 atagcggctg taccaaagcg cagatgatca actcctatga caactcggtg acctatgtgg
     7321 atcactttat ctccagcgtg attgatcagg ttcgcgataa gaaagcgatt gtgttctacg
     7381 cagctgacca cggtgagtca attaatgaac gcgagcacct gcacggcacg ccgcgtgaac
     7441 tggcaccgcc ggagcagttc cgcgtaccga tgatggtctg gatgtcagat aaatatctgg
     7501 aaaatccggc caatgcgcag gcgtttgcgc agctgaaaaa agaagccgac atgaaagtgc
     7561 cacgccgtca cgtagagctg tacgacacca tcatgggttg tcttggctat acttcaccgg
     7621 atggtggaat taacgaaaac aacaactggt gtcacatccc gcagacaaaa gaggcagcgg
     7681 ctaactaaga ggcctgctga ctttctcgcc gatcaaaagg cattttgcta ttaagggatt
     7741 gacgagggcg tatctgcgca gtaagatgcg ccccgcattc ggtgattggc gcagcttggt
     7801 agcgcacttc gttcgggacg aaggggtcgg aggttcgaat cctctatcac cgaccaaatt
     7861 cgaaaagcct gctcagcgag caggcttttt tgcatttgaa gcccatgagg atgagaacct
     7921 ccgggggcgg ggaggttcga ctcgagcgaa gcgagagaac gttgcgccag caacggcccg
     7981 cagggcgagc cacgaagtgg cgagtaatcc tctatcaccg accaaattcg aaaagcctgc
     8041 tcaacgagca ggcttttttg catttgtagc tcatgaggat gagaacctcc gggggcaggg
     8101 aggttcgact cgacgtaggc ctgataagac gcgccagcgt cgcatcaggc aacggcaccg
     8161 catttattgt cggatgcggc gcgaccgcct tattcgacca acacaccaca cattttcctg
     8221 ccaactttgc gccccatatc tcactatcca ttaacggatt tgtgacagaa accattgtct
     8281 ttttttatat ttgcttaaat ctttttttgc gttacttatg gctggcgctg gcattttccg
     8341 ctgtgcgttt tagcgttcat ggtattaatc gctcagatat ccaccttttc ctcagatttt
     8401 tttcattaaa tgcataaaac ttaatcacca aatgttgcta aatattaagc tgattgttct
     8461 ggttatgttg gagtagcaca aatttctctg ctgcaaatag ctgtttgaag tttttttaat
     8521 ctttgtttgt ggattctcac cgttggtgta aatcccggtt ggctgtattg acgttttcac
     8581 attctgttga cagattgtag gtcacgaggg gcattttatg gaggatccgc actgttacac
     8641 tgatgttaat tagtacggca tccccacctc ataacgttga cccgaccggg caaaaaacaa
     8701 aaaaggtcag gcagcgacaa cccactgcaa agggttaaaa caacaaacat cacaattgga
     8761 gcagaataat gcgtatttcc ttgaaaaagt cagggatgct gaagcttggt ctcagcctgg
     8821 tggctatgac cgtcgcagca agtgttcagg ctaaaactct ggtttattgc tcagaaggat
     8881 ctccggaagg gtttaacccg cagctgttta cctccggcac cacctatgac gcctcttccg
     8941 taccgcttta taaccgcctg gttgaattta aaatcggcac cactgaagtg atcccgggcc
     9001 tcgctgaaaa gtgggaagtc agcgaagacg gtaaaaccta taccttccat ctgcgtaaag
     9061 gtgtgaagtg gcacgacaat aaagaattca aaccgacgcg tgaactgaac gccgatgacg
     9121 tggtgttctc gttcgaccgt cagaaaaacg cgcaaaaccc gtaccataaa gtctctggcg
     9181 gcagctacga atacttcgaa ggcatgggct tgccggagct gatcagtgaa gtgaaaaagg
     9241 tggacgacaa cacagttcag ttcgtactga ctcgcccgga agcgccgttc ctcgctgatc
     9301 tggcaatgga cttcgcctct attctgtcaa aagaatatgc tgatgcgatg atgaaagccg
     9361 gtacaccgga aaaactggac ctcaacccaa tcggaaccgg tccgttccag ttacagcagt
     9421 accaaaaaga ttcccgtatc cgctacaaag cgtttgatgg ctactggggc accaaaccgc
     9481 agatcgatac gctggttttc tctattaccc ctgatgcttc cgtgcgttac gcgaaattgc
     9541 agaagaatga atgccaggtg atgccgtacc cgaacccggc agacatcgct cgcatgaagc
     9601 aggataaatc catcaacctg atggaaatgc cggggctgaa cgtcggctac ctctcctata
     9661 acgtgcagaa aaaaccgctg gatgacgtga aagttcgcca ggcactgacc tacgcggtga
     9721 acaaagacgc gatcatcaaa gcggtttatc agggcgcggg tgtatcagcg aaaaacctga
     9781 tcccgccaac catgtggggc tataacgacg acgttcagga ttacacctac gatcctgaaa
     9841 aagcgaaagc cttgctgaaa gaagcgggtc tggaaaaagg tttctccatc gacctgtggg
     9901 cgatgccggt acaacgtccg tataacccga acgctcgccg catggcggag atgattcagg
     9961 cagactgggc gaaagtgggc gtgcaggcca aaatcgtcac ctacgaatgg ggtgagtacc
    10021 tcaagcgtgc gaaagatggc gaacatcaga cggtaatgat gggctggact ggcgataacg
    10081 gggacccgga taacttcttc gccaccctgt tcagctgcgc cgcctctgaa caaggttcta
    10141 actactcaaa atggtgctac aaaccgtttg aagatctgat tcaaccggcg cgtgctaccg
    10201 acgaccataa caaacgcgtt gaactgtaca agcaagcgca ggtggtgatg cacgatcaga
    10261 ctccggcact gatcatcgct cactccaccg tgtttgaacc ggtacgcaaa gaagtcaaag
    10321 gctatgtggt tgatccatta ggcaaacatc acttcgaaaa cgtctctatc gaataattaa
    10381 aagccataca agactgatgg caaaggcaaa actgcctgat gtgctccgct tatcaggcct
    10441 acgaaaattc tgcaatgtat tgaatttgca cgattttgta ggccggataa ggcgttaacg
    10501 ccgcatccgg cataaacaaa gcgcactttg tcaacaatct gtatacccgg tggcgctgtg
    10561 cctctgcgtg cagcgccatc cggcagcaat acttcattcc ctgtccgata agggcgggaa
    10621 ttgatttgtg agcaatacag acacgcagtt ccaggctgcg ggtcactata gagaatccgg
    10681 gttatgttgc agtttattct ccgacgtttg ggactcgtca tccccacgtt tatcggtatt
    10741 acccttctca catttgcctt tgtccacatg atcccgggcg atccggtgat gatcatggca
    10801 ggcgaacgcg ggatctcccc tgagcgtcac gcgcagctgc tggctgaact cggcttagat
    10861 aaaccgatgt ggcagcagta tctccattac atttggggcg taatgcacgg cgatctaggc
    10921 atttcaatga aaagccgaat tccagtatgg gaagagttcg tgccgcgctt tcaggccacg
    10981 ctggaacttg gcgtctgcgc gatgattttt gctacggcag tcggtattcc ggttggtgtg
    11041 ctggctgcgg ttaaacgcgg ttccattttc gatcacactg cagttggcct ggcgctgacc
    11101 ggctactcga tgccgatctt ctggtggggc atgatgctga tcatgctggt ttcggtgcac
    11161 tggaacctga cgcccgtctc cggtcgcgtg agcgatatgg tgttcctcga tgactccaat
    11221 ccgttaaccg gttttatgct gatcgacacc gccatctggg gtgaagatgg caactttatc
    11281 gacgccgttg cccatatgat cttaccggct attgtgctgg gtactatccc gctggcggtt
    11341 atcgttcgta tgacgcgctc ttcgatgctg gaagtgctgg gtgaagatta catccgcacc
    11401 gcgcgcgcca aagggttgac ccgcatgcgg gtgattatcg tccatgcgct gcgtaacgcg
    11461 atgctgccgg tagtgaccgt tatcggcctg caggtgggaa cattgctggc gggggcgatt
    11521 ctgaccgaaa ccatcttctc gtggcccggt ctgggacgct ggttgattga cgcactgcaa
    11581 cgccgcgact atccggtagt gcagggcggc gtattgctgg tggcgacgat gattatcctc
    11641 gtcaacctgc tggtcgatct gctgtacggc gtggtgaacc cgcgtattcg tcataagaag
    11701 taaggggaca tcatgtcaca ggttactgaa aataaagtga ttagcgcacc ggtgccgatg
    11761 accccgttac aggagttctg gcactatttt aaacgcaaca aaggcgcggt tgtcgggctg
    11821 gtttacatcg tcatcgtgct gtttatcacg atctttgcca actggattgc gccttataac
    11881 ccggcggatc agttccgcga tgcgctgctc gccccgccag cctggcagga aggcggcagc
    11941 atggcgcact tgctgggcac cgatgacgta ggccgtgatg tgctgtcgcg gctgatgtac
    12001 ggcgcgcgct tgtcgctgct ggttggctgt ctggtggtgg tgttatcgct gattatgggc
    12061 gttattctcg gcctgatcgc cggttacttc ggcggcctgg tcgataacat catcatgcgc
    12121 gtggtcgata tcatgctggc gctgccaagt ctgctgttgg cactggtgct ggtggcgatt
    12181 ttcggcccgt cgattggtaa cgccgcgctg gcactgacct tcgttgcctt gccgcactat
    12241 gtgcgcttaa cccgcgccgc cgtgctggtg gaggtgaatc gcgattacgt taccgcgtct
    12301 cgcgtggcag gtgccggggc gatgcgtcag atgtttatta acatcttccc gaactgcctg
    12361 gcgccgctga ttgttcaggc gtcgctcggt ttctctaacg ccattctcga tatggccgcc
    12421 cttggcttcc tcggcatggg agcgcagccg ccaacaccag agtggggcac catgctctcc
    12481 gacgtgttgc agttcgcgca aagcgcctgg tgggtcgtga ccttcccggg tctggcgatc
    12541 ctgctgacgg tgctggcatt taacctgatg ggtgacggtc tgcgtgacgc gctcgatccc
    12601 aaactgaagc agtaagaggt tcgagatggc gttattaaat gtagataaat tatcggtgca
    12661 tttcggcgac gaaagcgcac cgttccgcgc cgtagaccgc atcagctaca gcgtaaaaca
    12721 gggcgaagtg gtcgggattg tgggtgagtc tggctccggt aagtcggtca gttcactggc
    12781 gattatgggg ctgattgatt atccgggccg cgtaatggcg gaaaagctgg tgtttaacgg
    12841 tcaggatttg cagcgtatat cagaaaaaga gcgccgcaac ctggtgggtg ccgaagtggc
    12901 gatgatcttc caggacccga tgaccagcct taacccgtgc tacaccgtgg gtttccagat
    12961 catggaagcg attaaggtgc atcagggcgg taataagagc actcgccgtc agcgggcgat
    13021 tgacctgctg aatcaggtcg gtattcccga tccggcatcg cgtctggatg tttacccgca
    13081 tcagctttcc ggcggcatga gccagcgcgt gatgatcgcc atggcgattg cctgtcggcc
    13141 aaaactgctg attgccgatg agccgaccac tgcgctggac gtgaccattc aggcgcaaat
    13201 catcgaactg ttgctggagc tacagcagaa agagaacatg gcgctggtgt taattaccca
    13261 tgatctggcg ctggtggcgg aagctgcgca taaaatcatt gtgatgtatg caggccaggt
    13321 ggtggaaacg ggcgatgcgc acgccatctt ccatgcgccg cgtcacccgt atactcaggc
    13381 attgctgcgt gcgctgccag aatttgctca ggacaaagaa cgtctggcgt cgttgccagg
    13441 tgtcgttccc ggcaagtacg accgcccgaa cggctgcctg cttaacccgc gctgccccta
    13501 tgccactgac agatgtcgcg ctgaagaacc ggcgctgaat atgctcgctg acgggcgcca
    13561 gtccaaatgc cattacccac ttgatgatgc cgggaggccg acactatgag tacgcaagag
    13621 gccaccctgc aacaaccgct gttgcaggct atcgacctga aaaaacatta tccggtgaag
    13681 aaaggcatgt tcgcgccgga acgtctggtt aaagcgctgg atggcgtttc gtttaacctt
    13741 gaacgtggca aaacgctggc agtagtgggc gaatctggct gcggtaaatc gaccctcggt
    13801 cggttgctga cgatgattga aacgcccacc ggtggcgagc tgtattacca ggggcaggat
    13861 ctgcttaagc acgatccgca ggcgcagaag ctgcgtcggc agaaaatcca gatcgtcttc
    13921 cagaaccctt acggttcgct gaatccgcgt aaaaaagtcg ggcaaattct tgaagagccg
    13981 ctgctgatca acaccagctt aagcaaagaa cagcgtcggg aaaaagccct gtcgatgatg
    14041 gcgaaagtcg gcctgaaaac cgagcactat gaccgctatc cgcatatgtt ctccggcggt
    14101 cagcgtcagc gtatcgccat cgcccgtggt ctgatgctcg acccggatgt ggtgattgcc
    14161 gatgaaccgg tttccgcgct ggatgtttca gtgcgcgcgc aggtgctgaa tctgatgatg
    14221 gatttgcagc aggagttggg gctgtcttat gtctttatct cccacgacct gtcggtggtg
    14281 gagcacattg ctgatgaagt gatggtgatg tacctgggcc gctgcgtgga gaagggaacg
    14341 aaagaccaaa tcttcaataa cccgcgccat ccgtacactc aggcgctact ttccgcgacg
    14401 ccgcgcctga acccggacga tcgccgcgag cgcatcaagc tcagcggtga actaccaagc
    14461 ccactgaatc caccgccggg ttgcgccttc aacgcccgct gtcgtcggcg cttcggcccc
    14521 tgcacccagt tgcagccgca gctaaaagac tacggcggtc aactggtagc ttgttttgct
    14581 gttgatcagg atgaaaatcc gcagcgttaa ctcacaaaac cggggccatg cgctccggtt
    14641 tactcaatca gcttcaacag tggcgacaag catagcaaaa tgccgtacag cagaatcagc
    14701 caggctttaa atccgcgaag tttttccagt tgtgcgactt tatagatcag gaaaaacgga
    14761 atgaggcacg acacaatacc atataacggg cttcccaact gaaagaacac cagcaccgat
    14821 acacgaaacg aaacccaaat cgtcaacgta atgacgataa aagcgcagat cgccagagtc
    14881 agcacgcgtg agttaatttt cttggtatca ataattcggc ttaacagatt gagaataatg
    14941 cctttaatgg cctcgtggaa accgagataa atgccaaaga atgcggtcag tacggcaaag
    15001 atattaagca ccgtagaggt gatatgaatg atatgcccag ggatcacctg cgcggccagc
    15061 gccagtgctg agatattttg ttcaaaggca gaaacggctt cttcgtggct aattgagaag
    15121 gtaaacgaaa aggcaaaaaa caggatcacc gcgatgagcg tgatataact aattcggtgg
    15181 gtacgcagcg cgagccgggt tgccagtact ttatccgctt cccgtttacg ataggcaata
    15241 ttcattgggt taagtacctg aataaatact gcagaaaaga agcaaaatgg aatggtaagc
    15301 aagacatcgc ggaaaaagac ggaggcttgc gggaaggcgg ttatattggc gaaattccag
    15361 tgcgggatca tcgcaaaacc gaacacgaca ataatcccta ctttgaccac caccattggc
    15421 ccggaaatct taaacagtaa tcgttcacca ccagacgcaa tcgccaccag tacggcgaaa
    15481 atagcgactt tataaagtag agattgtgaa agatcggcat cggttaaacc gaaggttttc
    15541 aggtacgagg cgctgtcgaa aaccacggag agagagtaga taaaaatccc gtggataatc
    15601 atcaaaaagt agataacccc gaggaaaatt ccccagttct tccccaggta atgactgata
    15661 atatcggtgt agtcattaca ggaatcgctt tcagaaaggg tttttaaata aatgtcctgc
    15721 actacccagg tggcaggata agcaatgatc gctgcggtaa taaataccca aattcccttc
    15781 aagccaattt gtactggcat cagcacggtt ccggcaccaa tcgccatgcc tatgcataat
    15841 aaaacccagc cgaagtcgta gcgtgtaaac ggcaatttct gattgtgttt cgatagtgtg
    15901 ttgtgctgca taaagtcctg tccagaaaga agtcggcctc gcaaaaatga ataaacccag
    15961 tttctcttgc gcaacaggtg gagaagtcga atacgcagta tgtaaaaaat gaggcaacag
    16021 attcactggg gaggtcgcga ggaggcgcaa ttctacaaaa ttgcgcattt cactaccagt
    16081 ctggagtcag cccggatatg aactgcaact attgcaaata aagaaattaa gctggaaggc
    16141 atcgcgcaat gaggaagatt gccgggtgct gacagtaaaa agcaatcgta aaatcagcgg
    16201 caagccgaaa gggttccggt gaggcgcaat gttgcggggg ctttatccct ggtggcattg
    16261 gttgctggag agagaaaacc cccgcacgtt gcaggtatgt acctgacaac ataacggggg
    16321 ctaatcttga acctagacca ctcaagaata gccgcgaaac gttgtcatta caacacaggc
    16381 ggctatatga cgctcgcaga gctgggcatg gccttctggc atgatttagc ggctccggtc
    16441 attgctggca ttcttgccag tatgatcgtg aactggctga acaagcggaa gtagcgtgtc
    16501 atgtgggcgt caggctgccg taatggcaat ttgcgcccgg accaggccgc aggagtgaaa
    16561 ctctgcggcc tttttcgtaa gaaaaatgca acggttgcaa gcggataata ttctgcggaa
    16621 tgcgcagcac agagtgaaga tgagacggat tccggaacag aaaaacagtc gtagaatcag
    16681 cgtcaagccg gaaggattcc ggtgaggcgc aatgttgcgg gggcttaatc cctggtggca
    16741 ttggttgctg gagagagaaa acccccgcac gt
//
LOCUS       DAKISA010000097        16263 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.097, whole genome shotgun sequence.
ACCESSION   DAKISA010000097 DAKISA010000000
VERSION     DAKISA010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16263
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.097"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(136..1164)
                     /gene="malI"
                     /locus_tag="OVW45_003254"
     CDS             complement(136..1164)
                     /gene="malI"
                     /locus_tag="OVW45_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCV5527628.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1339..2931
                     /gene="malX"
                     /locus_tag="OVW45_003255"
     CDS             1339..2931
                     /gene="malX"
                     /locus_tag="OVW45_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCV5527629.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            2941..4113
                     /gene="malY"
                     /locus_tag="OVW45_003256"
     CDS             2941..4113
                     /gene="malY"
                     /locus_tag="OVW45_003256"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCV5527630.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4217..5218
                     /gene="add"
                     /locus_tag="OVW45_003257"
     CDS             4217..5218
                     /gene="add"
                     /locus_tag="OVW45_003257"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCV5527631.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGVSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5254..6294)
                     /locus_tag="OVW45_003258"
     CDS             complement(5254..6294)
                     /locus_tag="OVW45_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCV5527632.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            6538..6663
                     /gene="blr"
                     /locus_tag="OVW45_003259"
     CDS             6538..6663
                     /gene="blr"
                     /locus_tag="OVW45_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCV5527633.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            6928..7143
                     /gene="ydgT"
                     /locus_tag="OVW45_003260"
     CDS             6928..7143
                     /gene="ydgT"
                     /locus_tag="OVW45_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCV5527634.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7229..7669
                     /locus_tag="OVW45_003261"
     CDS             7229..7669
                     /locus_tag="OVW45_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCV5527635.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            7746..8327
                     /gene="rsxA"
                     /locus_tag="OVW45_003262"
     CDS             7746..8327
                     /gene="rsxA"
                     /locus_tag="OVW45_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCV5527636.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFNGLVKL"
     gene            8327..8905
                     /gene="rsxB"
                     /locus_tag="OVW45_003263"
     CDS             8327..8905
                     /gene="rsxB"
                     /locus_tag="OVW45_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCV5527637.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            8898..10832
                     /gene="rsxC"
                     /locus_tag="OVW45_003264"
     CDS             8898..10832
                     /gene="rsxC"
                     /locus_tag="OVW45_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCV5527638.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQTNAEPEEQVDPRKAAVA
                     AAIARAQAKKAAQQKVVNED"
     gene            10833..11891
                     /gene="rsxD"
                     /locus_tag="OVW45_003265"
     CDS             10833..11891
                     /gene="rsxD"
                     /locus_tag="OVW45_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCV5527639.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSTLLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            11895..12515
                     /gene="rsxG"
                     /locus_tag="OVW45_003266"
     CDS             11895..12515
                     /gene="rsxG"
                     /locus_tag="OVW45_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCV5527640.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNTLAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            12519..13214
                     /gene="rsxE"
                     /locus_tag="OVW45_003267"
     CDS             12519..13214
                     /gene="rsxE"
                     /locus_tag="OVW45_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCV5527641.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            13214..13849
                     /gene="nth"
                     /locus_tag="OVW45_003268"
     CDS             13214..13849
                     /gene="nth"
                     /locus_tag="OVW45_003268"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCV5527642.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            14460..15962
                     /gene="dtpA"
                     /locus_tag="OVW45_003269"
     CDS             14460..15962
                     /gene="dtpA"
                     /locus_tag="OVW45_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCV5527643.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            16067..16186
                     /locus_tag="OVW45_003270"
                     /pseudo
     CDS             16067..16186
                     /locus_tag="OVW45_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
BASE COUNT         3782 a         4135 c         4148 g         4198 t
ORIGIN      
        1 tctcaagttg tcagaattaa acacataaac ctcctgtgaa cttcagtaaa aataaaaggc
       61 accgcctgtg tgccacattt tttatcatta accttgagga aaggacacta ccaggtatag
      121 cagataaaga gaggattatt tcgctgcaat gagccgggcg ggaataataa gattgcgtga
      181 atgcgtctct tcatgggtga ttttttgcat catgcgatcc gcaagtgtgg taccaagttc
      241 ccgcgcaggc gtgctggccc aggtaacggg tatatcatca agtgtggttg gtgtcgcatc
      301 ggtaaatgcc gccagcgaaa cttgttgctc aaagtaacga tcgactccgc tttccccgct
      361 ttgccgccct gctttcagca aaccaaacca tgcccccatc gcaatagttt cgttatagca
      421 aaccacagca ctgatggtcg ggttatgacg taaaagcgcc gtgatagctt ccgcggcttg
      481 cttctggctg gatgtgcact ccagcaccca atcgctgtga aacggcaggc caaattttag
      541 tagagttgca caatagcctc ccacccgttc tgcccgggtt aatgaggaac tttgccctcc
      601 cagccaggcg atccgctgat gcccattgcg aatgagatgc tccgtcaaca actgtgcagc
      661 ctgcatgttg tccgggcgaa ccgtatcaac atcatcaaga taactggcac gggaagcgaa
      721 aatcaccggg atagcttttt cttctgccat ccgtcgcagg tcatcgctgc ttcctgcagc
      781 cccggcaatt accacaccat cgacaccctg attcagtaac agtgaaaacc gctgtgccag
      841 ctgctcgccg tctttaccgc cgtgaagcaa aaaaaccatc cgtccctgcg cttccagagc
      901 ttccgtcaat ccggccgtca attcggcgta aaacggcgca gataaatcac ggacgatcaa
      961 accaatgacg ccgctttgcc cgccgcgcag cgccgacgcc tggcgattgc gcacaaatcc
     1021 cagctcttca atggcggcgt taacgcgttc tcctgtggcg gtagagattc gccctttgcc
     1081 actaagcacc agcgaaacgg tacttaccga cacgcccgca gccagcgcaa catcatgaat
     1141 ggttattttt ttggcggtag ccatgcagat gacctactcc ctgattatgt gacagataaa
     1201 acgttttacc ttttatttat cttatacccg ctattatcgt tgcgtaatgt gatttatgct
     1261 tcactaaaat ttgataaaac gttttatctt ctcgcgcaat ttactgaatc cagattgttc
     1321 tctacgagga gtcgttttat gacggcgaaa acagcaccga aagtcacgct gtgggagttc
     1381 ttccagcagt taggcaaaac cttcatgtta cccgtggcat tattgtcgtt ctgcggcatt
     1441 atgctcggca ttggtagttc tcttagtagc cacgatgtca tcaccctgat cccggtcctg
     1501 ggcaaccccg tgttgcaggc tatctttacc tggatgagta agattggctc gtttgctttt
     1561 agtttcctgc cagtcatgtt ctgtatcgcc atcccgctgg gtctggcacg cgaaaacaaa
     1621 ggcgtagcgg cattcgctgg cttcgtcggt tatgcggtaa tgaacctcgc ggtaaacttc
     1681 tggttgacca ataaaggcat tctgccaacc acggacgccg cggttctgaa agccaataac
     1741 atccagagca ttcttgggat ccagtcgatc gacaccggga tcctcggtgc ggtgatcgcc
     1801 ggtattatcg tctggatgct gcatgagcga ttccataata tccgcctgcc ggatgcgctt
     1861 gcattcttcg gcggtacgcg cttcgtacca atcatctcct cgctggtgat gggccttgtc
     1921 ggcctggtga ttccattagt ctggccgatt ttcgcgatgg gtattagcgg cttgggccat
     1981 atgataaaca gcgcgggtga tttcggaccg atgctgtttg gtaccggtga acgtctgctg
     2041 ttgccgtttg gtctgcatca cattctggtg gcattaattc gctttaccga cgcaggcggc
     2101 acgcaggaag tctgcggtca aaccgtcagc ggcgcattga ccatcttcca ggcgcaattg
     2161 agttgcccga ccactcacgg tttttctgaa agcgccacgc gtttcctttc gcaaggcaaa
     2221 atgcctgcgt ttctcggcgg tctgccaggt gcagcgttag cgatgtatca ctgcgcgcgc
     2281 ccggaaaatc gccataaaat taaagggctg ctgatttctg gcctgatcgc ctgtgtcgtt
     2341 ggcggcacga ccgaaccgct ggaattcctg ttcctgttcg tggcgccagt tctgtatgtc
     2401 atccacgcgc tgttaaccgg cctcggcttc actgtcatgt ctgtgctcgg cgtcaccatc
     2461 ggtaataccg acggcaatat catcgacttc gtggtgttcg gtattttgca tggtctgtca
     2521 accaagtggt acatggtgcc agtggtggcg gcaatctggt ttgtcgttta ctacgtcatc
     2581 ttccgtttcg ctatcacccg cttcaatctg aaaaccccgg ggcgcgatag cgaagtggcc
     2641 agttcaatcg aaaaagccgt tgccggtgcg ccgggtaaat caggttacaa cgttccggca
     2701 atcctcgaag ccttaggcgg tgccgacaat attgttagcc tcgataactg cattacccgt
     2761 ctgcgtttgt ctgtgaaaga tatgtcgctt gttaatgtgc aggcactgaa ggacaatcgg
     2821 gcaattggcg tagtacaact taatcaacat aacctgcagg ttgttatcgg gccacaagtt
     2881 cagtcagtaa aagatgaaat ggccggtctg atgcatactg tccaggcata aggataagat
     2941 atgttcgatt tttcaaaggt cgtggatcgt catggcacat ggtgtacaca gtgggattat
     3001 gtcgctgacc gtttcggcac tgctgacctg ttaccgttca cgatttcaga catggatttt
     3061 gccactgccc cctgcattat cgaggcgctg aatcagcgcc tgatgcacgg cgtatttggc
     3121 tacagccgct ggaaaaacga tgagtttctc gcggctattg cccactggtt ttccacccag
     3181 cattacaccg caattgatac gcagacggtg gtgtatggcc cttctgtcat ctatatggtt
     3241 tcagaactga ttcgtcagtg gtctgaaaca ggtgaaggcg tggtgatcca cacgcccgcc
     3301 tatgacgcat tttacaaggc cattgaaggt aaccagcgca cagtaatgcc cgttgcttta
     3361 gagaagcagg ctgacggctg gttttgcgat atgggcaagc tggaagccgt tttggcgaaa
     3421 ccagaatgta aaattatgct cctgtgtagc ccacagaatc ctaccgggaa agtgtggacg
     3481 tgcgatgagc tggagatcat ggctgacctg tgcgagcgtc atgatgtgcg ggttatttcc
     3541 gatgaaatcc atatggatat ggtctggggc gagcagccgc atattccctg gagtaatgtg
     3601 gcgcggggag actgggcgtt gctgacgtcg ggatcgaaaa gtttcaatat tcccgcgctg
     3661 accggtgctt acgggattat agaaaatagc agcagccgtg atgcctattt atcggcactg
     3721 aaaggacgtg atgggctttc ttccccttcg gtactggcgt taactgccca tatcgccgcc
     3781 tatcagcaag gtgcaccgtg gctggatgcc ttacgcgtct atctgaaaga taacctgacg
     3841 tatatctcag ataaaatgaa cgccgcgttt cctgaactca actggcagat cccgcaatcc
     3901 acttatctgg cctggcttga tctacgtccg ttgaatattg acgacaacgc gttgcaaaaa
     3961 gcgcttatcg agcaagaaaa agtcgcgatc atgccggggt atacctatgg tgaagaaggt
     4021 cgtggttttg tccgtctcaa tgccggctgc ccacgttcga aactggaaaa aggtgtggct
     4081 ggattaataa acgccatccg cgctgttcgc taaccccaat tgcgcaacgt aaaaaatcgt
     4141 tgcgcaatcg tggattttta ccctgctttg tttttataat ggtgcgcact tttatatcca
     4201 gaaaaagagt gcgaccatga ttgataccac cctgccatta actgatatcc atcgccacct
     4261 tgatggcaac attcgtcccc agaccattct tgaacttggc cgccagtata atatctcgct
     4321 tcctgcacaa tccctggaaa cactgattcc ccacgttcag gtcattgcca acgaacccga
     4381 tctggtgagc tttctgacca aacttgactg gggcgttaaa gttctcgcct ctcttgatgc
     4441 ctgccgccgc gtggcatttg aaaacattga agatgcagcc cgtaacggcc tgcactatgt
     4501 cgagctgcgt ttttcaccag gctacatggc aatggcacat cagctgcctg tagcgggtgt
     4561 tgtcgaagcg gtgatcgatg gcgtacgtga aggttgccgc acctttggtg tgcaggcgaa
     4621 gcttatcggc attatgagcc gaaccttcgg cgaagccgcc tgtcagcaag agctggaggc
     4681 ctttttagcc caccgtgacc agattaccgc acttgattta gctggtgatg aacttggatt
     4741 cccgggaagt ctgttccttt ctcacttcaa ccgcgcgcgt gatgcgggct ggcatattac
     4801 cgtccatgca ggcgaagctg ccgggccgga aagcatctgg caggcgattc gtgaactggg
     4861 tgcggagcgt attggacatg gcgtaaaagc cattgaagat cgggcgctga tggattttct
     4921 cgccgagcaa caaattggta ttgaatcctg tctgacctcc aatattcaga ccagcaccgt
     4981 agcagagctg gctgcacatc cgctgaaaac gttccttgag catggcattc gtgccagcat
     5041 taacactgac gatcccggcg tacagggagt ggatatcatt cacgaatata ccgttgccgc
     5101 gccagctgct ggggtatccc gtgagcaaat ccgccaggca cagattaatg gtctggaaat
     5161 ggctttcctc agcgcagagg aaaaacgcgc actgcgagaa aaagtcgccg cgaagtaaca
     5221 aaaatggatg gtgcaatttg caccatccat ttttcacgca aggcacaacg tcgcgcgatg
     5281 tttggcggat tcgatgccca gctcaatcaa ctccattacc tggattgcct ggcttgccgg
     5341 aaccggattt tcaccatcgc catttaacgc atcacgaatc gccgcatagt aagccggata
     5401 attcccaggc accgtcaaca gcgtttcttc gacacgttcc tcaccttcca cgcgggtaag
     5461 tacgccatca cgcatatcgt agccccagtc ttcctgcggc agacgctcgc catttttcag
     5521 acgttcttcc tgtggatcga ggccatattt cacataactg cctcgggatc catgcacgat
     5581 ataccgtgct gactcagcgg ctgccagcat ggtaccgtgt aaaatgactc gccgctgtgg
     5641 ataggacaag atggcgtgga aataatcggt cgactgcgct ccgggccgta actgtgccaa
     5701 atctaccgtc atgctgaccg gtaaaccaaa tagcgtaatg gcctgatcaa gaagatgcgg
     5761 tgctaaatcg taccagatac cgctgcctgg gccgccctgt tcacgccaac gatcgcgcac
     5821 ctgcggacgg aagcggtcaa aatgagactc aaagtaagca acttcaccca gcacgccttc
     5881 cgcgagcaaa ccttttagcg tcaagaaatc gctatcccaa cgacggttat ggaatacaga
     5941 cagcacacgc cccaggcttt ttgccagcgc atccagctct cgcgcttgtg acagtgtcac
     6001 ggtaaagggt ttatcaacga ccacatgttt acccgcctca agcgccgctt tggctaacgg
     6061 gaaatgggta tcgttgggtg taggaatgac aatcaggtct atgttgggat cgttaaacag
     6121 atgcttcggc tcagagacaa ccgtaaccgt tggccagtcg gcttttactt ttgtttcatc
     6181 actgctggag attaccgcca gttccagccc gggcgtgccc gcaatcaggg gcgcatggaa
     6241 ggttttgctc gcataaccat acccaatcaa cccaacacgg atgttgtcgc tcatgtcatt
     6301 gcctctcatt gaagtatgat ggctatttga cactatcctt tacccacgct caacagttta
     6361 ataacctgcc agcaataagg gatgttgttt aacttaagtc aaaaaaaata gcgaattttc
     6421 caacgacaaa agctaaatat cgcaaaaacc tcagtaaaaa tcttgctgga gctattattg
     6481 ctaagtaaca tttaccccct gaagttaatg gatcaatcaa gagagatgtg ggctgtaatg
     6541 aatcgtctta ttgaattaac aggttggatc gttcttgtcg tttcagtcat tcttcttggc
     6601 gtggcgagtc acattgacaa ctatcagcca cctgaacaga gtgcttcggt acaacacaag
     6661 taagctctgc acttgtggag cgacatgctg cccgtccggg tgcatgtttt cacttgtcgg
     6721 atattaaacc aggaatttat tatcttgttc gatgttgttg gtgattgtca gggatagtaa
     6781 agcgcgttac tcttttcaga gcagtttttc atccctgaat atttgccatc agcgcattct
     6841 tattattgta ataacacaag cgttcacttt tggtgaaatt ttatcatcac tctacccctt
     6901 tggtattaat tattttatgg accttttatg actgttcagg actacttatt aaaatttcgc
     6961 aaaatcagtt cactcgaaag tctggaaaaa ctctacgacc atcttaatta caccctgacg
     7021 gacgatcagg aactgatcaa tatgtatcgt gctgccgatc accgtcgcgc agagctggtt
     7081 tctggcgggc gtttgtttga cctcggccag gtaccgaagt ccgtctggca ctatgtccaa
     7141 taaagaaagt agcgatcggt tataaaagcc ttataataac gcccctaaaa ttagatttgc
     7201 ctgtttgcgt cgctattctg gagaacctat gaccaccaca acgccacaac gtattggagg
     7261 ctggttgctt ggcccgttgg cctggctttt agtcgctctg ttgagtacga cgctggcgtt
     7321 gttgctgtat accgctgcgt tatcttctcc gcaaacattt caaacgctcg gcggacaagc
     7381 actgacgaca caaatcttat ggggcgtttc ttttattacc gctatcgcca tgtggtatta
     7441 cacactgtgg ttgaccatcg ctttctttaa acgtcggcgt tgtgttccta aacactatat
     7501 tatctggctg cttatttccg tattgctggc agtaaaagca ttcgcttttt cacccgtgga
     7561 agatggcata gccgttcgcc agttgctgtt tactttgttg gcaaccgcat tgatcgtccc
     7621 ctatttcaaa cgctcgtcgc gggtaaaagc gacgtttgtg aatccgtaat aaccttacag
     7681 ttaacctgtt gtcgcctgct ctggattaac ggataatagg cggctttttt atttcaggcc
     7741 gaaaaatgac tgactacctg ttactgtttg tcggaactgt actggtcaat aactttgtac
     7801 tggtcaagtt tctcggtctc tgtccgttta tgggggtttc caaaaaactg gaaaccgcga
     7861 tgggcatggg gctggcaaca acgtttgtga tgacgctggc gtctatttgc gcctggctta
     7921 tcgatacgtg gattttgatc ccacttaatc tgatttacct gcgcaccctg gcatttattc
     7981 tggtgattgc tgtggtcgtg cagttcaccg agatggtggt gcgcaaaacc agcccggtgc
     8041 tttaccgtct gctggggatt tttttgccgc ttatcaccac caactgtgca gtgctcggcg
     8101 tggcgttgct gaatatcaat ctcgggcaca atttcttgca gtcggcgctg tacggttttt
     8161 ccgccgctgt cggtttctcg ctggtgatgg tgctcttcgc cgccatccgc gaacgccttg
     8221 ctgtggctga tgtcccggca ccttttcgcg gtaatgccat tgcgttaatt accgcaggtc
     8281 ttatgtctct ggcctttatg ggctttaatg gtttggtgaa gttgtaatga atgctatctg
     8341 gattgccgtt gccgccgtga gcctgctggg cctggcgttt ggcgccattc tgggttatgc
     8401 ctcccgccgt tttgcggtgg aagacgatcc ggtcgttgag aaaattgacg aaatcttacc
     8461 gcagagccag tgtggtcagt gcggttatcc cggctgtcgc ccctacgcgg aagccatcag
     8521 ctgtaacggt gaaaaaatca accgttgcgc cccaggtggc gaagctgtga tgctaaaaat
     8581 tgccgagttg cttaatgtcg agccgcagcc gctggatggc gaagcgcaag agctaacgcc
     8641 tgcgcggatg gtggcggtta ttgatgaaaa taactgtatt ggctgcacta aatgtattca
     8701 ggcttgtccg gtagacgcca tcgttggcgc tacccgtgcc atgcatacgg taatgagtga
     8761 tctctgtacg ggctgcaatt tatgtgttga tccgtgcccg acgcactgca tctcgttgca
     8821 accggtcgca gaaacacctg actcctggaa atgggatctg aacaccattc ccgtgcgtat
     8881 cattcccgtg gaacaccatg cttaagttat tctctgcatt cagaaaaaat aaaatctggg
     8941 atttcaacgg cggcatccat ccaccggaga tgaaaaccca gtccaacggt acacccctgc
     9001 gccaggtacc actggcgcag cgttttgtta ttccactgaa acagcatatt ggcgctgaag
     9061 gtgagttgtg cgttagcgtc ggcgataaag tattgcgcgg ccagccgctt acccgtggtc
     9121 gcggcaaaat gctgcctgtt cacgcgccca cctcgggtac cgttacggct attgcgcccc
     9181 actctacggc tcatccttca gctttagctg aattaagcgt gattattgat gccgatggtg
     9241 aagactgctg gatcccgcgc gacggctggg ccgattatcg cactcgcagt cgcgaagagt
     9301 taatcgagcg catacatcag tttggtgttg ccgggctggg cggtgcagga ttcccgacag
     9361 gcgttaaatt gcagggtggc ggagataaga ttgaaacgtt gattatcaac gcggctgagt
     9421 gcgagccgta cattaccgcc gatgaccgtt tgatgcagga ttgcgcggct caggtcgtag
     9481 agggtattcg cattcttgcg catattctgc agccacgcga aattcttatc ggcattgaag
     9541 ataacaaacc gcaggcgatt tccatgctgc gcgcggtgct ggcggactct aacgatattt
     9601 ctctgcgggt gattccaacc aaatatcctt ctggcggtgc taaacaatta acctacattc
     9661 tgaccgggaa gcaggttcca catggcgggc gttcatccga tatcggcgta ttaatgcaaa
     9721 acgtcggcac tgcttatgca gtgaaacgtg ccgttattga tggcgagccg attaccgagc
     9781 gtgttgtaac cctgactggc gaagcaatcg ctcgcccggg caacgtctgg gcacggctgg
     9841 ggacgccagt gcgtcattta ttgaatgatg ccggattctg cccctctgcc gatcaaatgg
     9901 tgattatggg tggcccgcta atgggcttta ccttgccatg gctggatgtc ccggtcgtaa
     9961 agattaccaa ctgtctgttg gctccctctg ccaatgaact tggcgaacca caggaagaac
    10021 aaagctgcat ccggtgtagc gcctgtgctg acgcctgccc tgcggatctt ttgccgcaac
    10081 agttgtactg gttcagcaaa ggtcagcaac acgataaagc taccacgcat aacattgctg
    10141 attgcattga atgtggggct tgcgcgtggg tttgcccgag caatattccc ctggtgcaat
    10201 atttccgtca ggaaaaagct gaaattgcgg ctattcgtca ggaagaaaag cgcgccgcag
    10261 aagccaaagc gcgtttcgaa gcgcgccagg ctcgtctgga gcgcgaaaaa gcggctcgcc
    10321 ttgaacgaca taagagcgca gccgttcaac ctgcagccaa agataaagat gcgattgctg
    10381 ccgctctggc gcgggtgaaa gagaaacagg cccaggctac acagcctatt gtgattaaag
    10441 cgggcgaacg cccggataac agtgcaatta ttgcagcacg ggaagcccgt aaagcgcaag
    10501 ccagagcgaa acaggcagaa ctgcagcaaa ctaacgacgc agcaaccgtt gctgatccac
    10561 gtaaaactgc cgttgaagca gctatcgccc gcgccaaagc gcgcaagctg gaacagcaac
    10621 aggctaatgc ggaaccagaa gaacaggtcg atccgcgcaa agccgccgtc gaagccgcta
    10681 ttgcccgtgc caaagcgcgc aagctggaac agcaacagac taacgcagaa ccagaagaac
    10741 aggttgatcc gcgcaaagcg gcagttgccg cggctattgc ccgcgctcag gccaaaaaag
    10801 ccgcccagca gaaggttgta aacgaggact aaatggtatt cagaatagct agctcccctt
    10861 atacccataa ccagcgccag acatcgcgca ttatgctgtt ggtgttgctc gcagccgtgc
    10921 caggaatcgc agcgcaactg tggttttttg gttggggtac tctcgttcag atcctgttgg
    10981 catcggttag tactctgtta gccgaagctc tcgtactcaa actacgcaag cagtcggtag
    11041 ccgcaacgtt gaaagataac tcagcattgc tgacaggctt attgctggcg gtaagtattc
    11101 cccccctcgc gccatggtgg atggtcgtgc tgggtacggt gtttgcggtg atcatcgcta
    11161 aacagttgta tggcggtctg gggcaaaacc cgtttaatcc ggcaatgatt ggttatgtgg
    11221 tcttactgat ctccttcccg gtgcagatga ccagctggtt accgccacat gaaattgcgg
    11281 tcaacatccc tggttttatc gacgccatac aggttatttt cagcgggcat accgccagtg
    11341 gtggtgatat gaacacacta cgcttaggta ttgatggcat tagtcaggcg acaccgctgg
    11401 atacatttaa aacctctgtc cgtgccggtc attcggttga acagattatg caatatccga
    11461 tctacagcgg tattctggcg ggcgctggtt ggcaatgggt aaatctcgcc tggctggctg
    11521 gcggcctgtg gttgctatgg cagaaagcga ttcgctggca tattcccctc agcttcttag
    11581 taacgctggc gttatgcgca acgttgggct ggttgttctc accagaaaca ctggcagcac
    11641 cgcaaattca tctgctgtct ggtgcgacca tgctcggcgc attctttatt ttgactgacc
    11701 cggttaccgc ttctacgacc aatcgtggtc gtcttatttt cggcgcgctt gcgggcttat
    11761 tagtctggtt gatccgcagt ttcggcggct atcctgacgg cgtggctttt gccgtcctgc
    11821 tggcgaacat cacggttcct ctgatcgatt actacacgcg tccgcgcgtc tacggccatc
    11881 gcaaagggta aaccatgctg aaaactatcc gaaaacacgg cattacgtta gcgctatttg
    11941 cagcgggttc aacagggtta actgcggcca tcaaccagat gaccaaaacg acgattgctg
    12001 aacaggccag tctgcaacaa aaggcattat ttgatcaggt gctgccagcc gaacgctata
    12061 acaatacgct ggcacagagt tgctatctgg taactgcgcc agagttaggt aaaggtgagc
    12121 atcgggttta catcgccaaa caggatgaca aaccggtagc cgccgttctg gaagcaaccg
    12181 cgccagatgg ctattccggt gcgattcagc tgctggtggg agccgatttt aacggcacgg
    12241 tacttggcac gcgcgtgaca gagcaccacg aaacgccagg gcttggcgat aaaatcgaac
    12301 tgcgcctttc tgactggatc acccattttg cgggtaaaaa aatcagtggt gcggatgatg
    12361 cgcactgggc ggtgaagaaa gatggtggtg atttcgacca gttcaccggc gcgacgatta
    12421 ctccccgcgc ggtggttaat gcggtaaaac gcgccggatt gtacgctcag acgttaccgg
    12481 cacaactttc tcaacttcct gcctgtggag aataaagcgt gagcgaaatt aaagacgtta
    12541 ttgttcaggg gttgtggaaa aacaactctg cgctggtcca gttgctcggc ctttgtcctc
    12601 tgttggcggt cacgtccact gccactaacg ctctgggttt aggacttgcg actacgctgg
    12661 tactgacgct gaccaacctg accatttcga cgctgcgtca ctggacgcca gccgagatcc
    12721 gcattcccat ttacgtgatg atcatcgcct cggtggtcag cgctgtacag atgctgatca
    12781 acgcctacgc ctttggcctg tatcaatcat tagggatttt tattccgctg attgtcacta
    12841 actgtatcgt tgtgggccgc gctgaagcct tcgccgccaa aaaaggtccg gcgctttcgg
    12901 cactggacgg cttttcaatt ggtatgggcg caacctgcgc catgttcgtg ctgggttcac
    12961 tacgcgaaat tatcggcaat ggcacattgt ttgacggtgc agatgcgctg ttaggtagct
    13021 gggcaaaagt attacgcgtg gagattttcc acaccgactc ccctttcctg ctggcgatgc
    13081 tgccaccagg tgcatttatt ggcctgggac tgatgctggc aggaaaatac ctgattgatg
    13141 aaagaatgaa aaagcgccgt actgaagcag ctgccgaacg tgcattgcca aacggtgaaa
    13201 cagggaatgt ctgatgaaca aagcaaaacg cctggagatc ctcactcgcc tgcgtgagaa
    13261 caatcctcat cccaccaccg agcttaattt cagttcgcct tttgaattgc tgattgccgt
    13321 actgctttcc gctcaggcga ccgatgtcag tgttaataag gcgacggcga aactttaccc
    13381 ggtggcgaat acgcctgcag cgatgcttga actgggcgtt gaaggggtta aaacctatat
    13441 caaaacgatt gggctttata acagcaaagc agaaaatatc atcaaaacct gccgtatctt
    13501 actggagcag cataatggcg aggttccgga agatcgtgct gcgcttgaag ccctgcccgg
    13561 cgtaggtcgt aaaacagcca acgtcgtatt aaacactgca ttcggctggc cgactattgc
    13621 tgtcgacacg cacattttcc gcgtttgtaa tcgtactcaa tttgcgccgg ggaaaaacgt
    13681 cgaacaggta gaagaaaagc tactgaaagt ggttccggca gagtttaaag tcgactgcca
    13741 ccattggttg atcctgcacg ggcgttatac ctgcattgcc cgcaagcccc gctgtggctc
    13801 ttgtattatt gaagatcttt gtgaatacaa agagaaagtt gacatctgaa gaaaaggggt
    13861 aacaccgatt accccattga taacctttct ttatcctctt ttaaaacatc tttttaacgt
    13921 gaatgatgcc attgcttagc gttatcatca ggtaatccgt ttgcggataa ccaaaaatgc
    13981 aggttaattg tttttttaat agcgaaattt tctattcatt cgtgatcaag atcacgatgc
    14041 tgtcgcaatg taaaaacatt gcaagaatca ggttaaaata atttaaattt acacacgcaa
    14101 caaatattga cctacaaaac attacactgg ctatttttca gaaactggac tatctcacta
    14161 gtaaaaacgc taaatagcag aacatatcgc cgttaagcca tcacccccct ctcagtgcag
    14221 tgaaaaaatc tgccattacg ttttttgaaa aatttaacgc tggataacat ttcccggaat
    14281 ggttgaattc cccgcctcag ttatatgtaa cagattatta caaaggactt gtctgaaagt
    14341 gcaagatagt gaacattacc tgccgtttcc cctcccacta taacaattgc gcgtatgttt
    14401 tttagacata acgcgagaaa gcacccccgt taatatggga tgtaaaaaaa gaggtaaaag
    14461 tgtccactgc aaaccaaaaa ccaactgaaa gcgtcagttt gaacgctttc aaacaaccga
    14521 aggcgttcta tctcatcttc tcgattgagt tatgggaacg ttttggttat tacggcctac
    14581 aaggaattat ggctgtttac ctggttaaac aactgggtat gtctgaagcg gattcaatca
    14641 cccttttctc ttcctttagt gccctggttt atggtctggt cgctatcggc ggctggttag
    14701 gtgacaaggt actgggtact aaacgcgtaa ttatgctcgg cgctattgtg ctggcgattg
    14761 gttatgctct ggttgcctgg tctggtcacg acgccggtat cgtttatatg ggtatggcgg
    14821 ctattgcggt cggtaacggc ctgtttaaag ctaacccatc ttctctgctt tctacatgct
    14881 atgagaaaaa cgacccgcgt ctggacggtg cattcaccat gtactacatg tccgtcaaca
    14941 tcggctcttt cttctctatg attgctacgc cgtggctggc cgcgaaatac ggctggagtg
    15001 ttgcgtttgc gttgagcgtt gtaggcctgc tgatcactat cgttaacttc gccttctgcc
    15061 aacgctgggt taaacagtac ggttcaaaac cagacttcga gcctatcaac taccgtaacc
    15121 tgctgctgac cattattggt gttgtggcac tgatcgctat cgccacctgg ctgctgcaca
    15181 atcaggaagt tgcgcgtatg gcgctgggcg ttgttgcctt cggtatcgtg gttatcttcg
    15241 gtaaagaagc cttcgcgatg aaaggtgctg cgcgtcgtaa aatgatcgtt gccttcatcc
    15301 tgatgctcga agccattatc ttcttcgtgc tgtacagcca gatgccaacg tcactgaact
    15361 tctttgcgat tcgtaacgtt gagcactcca ttctgggtct ggccgtagaa cctgagcagt
    15421 atcaggcact gaacccgttc tggatcatca tcggtagtcc gattctggcc gctatctata
    15481 acaagatggg cgataccctg ccgatgccaa ccaagtttgc aatcggcatg gtgatgtgtt
    15541 ctggtgcgtt cctgattctg ccgctgggtg cgaaattcgc gtctgacgct ggtatcgtgt
    15601 ctgtaagctg gctggtcgca agctatggcc tgcagagcat cggggaactg atgatctctg
    15661 gtctgggtct ggcaatggtt gcacaactcg ttccgcagcg tctgatgggc ttcattatgg
    15721 gtagctggtt cctgaccact gccggtgcaa acctgattgg tggttatgtt gcgggtatga
    15781 tggctgtgcc ggataacgtt accgacccgc tgatgtcact ggaagtttat ggtcgcgtat
    15841 tcttgcagat tggtgtcgct actgccatta ttgcagtact gatgctgctg accgcgccta
    15901 aactgcaccg tatgacgcag gatgacgctg cagacaaagc ggcgaaagct gctgtagcgt
    15961 aaaattcagg gaaactcttt tacaagccgc taacttttcg ttagcggctt ttttttgttc
    16021 agcaacgcac taccatactt taaaccacag acaaaaggag ttaccgatga aattgttcta
    16081 caaaccgggt gcctgctctc tcgcttccca tatcaccctg cgtgagagcg gaaaggattt
    16141 taccctcgtc agtgtggatt taatgaaaaa acgtctcgaa aacggtgtaa tgccgatcag
    16201 ttaaggatca gttgaccgat ccagtggctg tgtaagaatc cggaaacgct cacttgtttc
    16261 cgg
//
LOCUS       DAKISA010000098        16252 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.098, whole genome shotgun sequence.
ACCESSION   DAKISA010000098 DAKISA010000000
VERSION     DAKISA010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16252
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.098"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..578
                     /locus_tag="OVW45_003271"
     CDS             <1..578
                     /locus_tag="OVW45_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCV5527644.1"
                     /translation="GRPNNPFEPRRNRYAQKYLTLALMGGAAFFVLKGCGDSSDVDND
                     GDGTFYATVQDCIDDGNNADICARGWNNAKTAFYADVPKNMTQQNCQSKYENCYYDNV
                     EQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASRPVWRNTSGDYSWRSGSGKKES
                     YSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            581..1744
                     /locus_tag="OVW45_003272"
     CDS             581..1744
                     /locus_tag="OVW45_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCV5527645.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWHASDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIDSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            1828..3453
                     /locus_tag="OVW45_003273"
     CDS             1828..3453
                     /locus_tag="OVW45_003273"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCV5527646.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQVLCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(3570..3845)
                     /gene="yjfN"
                     /locus_tag="OVW45_003274"
     CDS             complement(3570..3845)
                     /gene="yjfN"
                     /locus_tag="OVW45_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCV5527647.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3994..4323)
                     /gene="bsmA"
                     /locus_tag="OVW45_003275"
     CDS             complement(3994..4323)
                     /gene="bsmA"
                     /locus_tag="OVW45_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCV5527648.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4505..5254
                     /gene="yjfP"
                     /locus_tag="OVW45_003276"
     CDS             4505..5254
                     /gene="yjfP"
                     /locus_tag="OVW45_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCV5527649.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5251..6006)
                     /gene="ulaR"
                     /locus_tag="OVW45_003277"
     CDS             complement(5251..6006)
                     /gene="ulaR"
                     /locus_tag="OVW45_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCV5527650.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ERGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6114..7178)
                     /gene="ulaG"
                     /locus_tag="OVW45_003278"
     CDS             complement(6114..7178)
                     /gene="ulaG"
                     /locus_tag="OVW45_003278"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCV5527651.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7533..8930
                     /gene="ulaA"
                     /locus_tag="OVW45_003279"
     CDS             7533..8930
                     /gene="ulaA"
                     /locus_tag="OVW45_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCV5527652.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWTAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            8946..9251
                     /gene="ulaB"
                     /locus_tag="OVW45_003280"
     CDS             8946..9251
                     /gene="ulaB"
                     /locus_tag="OVW45_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCV5527653.1"
                     /translation="MTVRILAVCGNGQGSSMIMRMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9261..9725
                     /gene="ulaC"
                     /locus_tag="OVW45_003281"
     CDS             9261..9725
                     /gene="ulaC"
                     /locus_tag="OVW45_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCV5527654.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            9739..10389
                     /gene="ulaD"
                     /locus_tag="OVW45_003282"
     CDS             9739..10389
                     /gene="ulaD"
                     /locus_tag="OVW45_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCV5527655.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10399..11253
                     /gene="ulaE"
                     /locus_tag="OVW45_003283"
     CDS             10399..11253
                     /gene="ulaE"
                     /locus_tag="OVW45_003283"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCV5527656.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGTVEAA"
     gene            11253..11939
                     /gene="ulaF"
                     /locus_tag="OVW45_003284"
     CDS             11253..11939
                     /gene="ulaF"
                     /locus_tag="OVW45_003284"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCV5527657.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            12036..12587
                     /locus_tag="OVW45_003285"
     CDS             12036..12587
                     /locus_tag="OVW45_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HCV5527658.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(12662..12937)
                     /locus_tag="OVW45_003286"
     CDS             complement(12662..12937)
                     /locus_tag="OVW45_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCV5527659.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            13264..13659
                     /gene="rpsF"
                     /locus_tag="OVW45_003287"
     CDS             13264..13659
                     /gene="rpsF"
                     /locus_tag="OVW45_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCV5527660.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13666..13980
                     /gene="priB"
                     /locus_tag="OVW45_003288"
     CDS             13666..13980
                     /gene="priB"
                     /locus_tag="OVW45_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCV5527661.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13985..14212
                     /gene="rpsR"
                     /locus_tag="OVW45_003289"
     CDS             13985..14212
                     /gene="rpsR"
                     /locus_tag="OVW45_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCV5527662.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            14254..14703
                     /gene="rplI"
                     /locus_tag="OVW45_003290"
     CDS             14254..14703
                     /gene="rplI"
                     /locus_tag="OVW45_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCV5527663.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14815..15786)
                     /locus_tag="OVW45_003291"
     CDS             complement(14815..15786)
                     /locus_tag="OVW45_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HCV5527664.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKNKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMESFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(15839..>16252)
                     /locus_tag="OVW45_003292"
     CDS             complement(15839..>16252)
                     /locus_tag="OVW45_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCV5527665.1"
                     /translation="WAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIGTFMDMT
                     PAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVSKLPINK
                     IIKPMLPFYAVMVLVLAMVTYIPQISMALPKALGY"
BASE COUNT         3817 a         3968 c         4324 g         4143 t
ORIGIN      
        1 ttggtagacc gaataatcct tttgaaccgc gtcgcaatcg ctacgcacaa aaatacttaa
       61 cgttggcgct aatgggcggt gccgcttttt tcgtattgaa aggttgtggc gatagtagcg
      121 atgtcgataa cgacggcgac ggaacgtttt acgcgacagt gcaggattgt attgatgacg
      181 gtaataatgc cgacatttgc gcgcgtggct ggaacaacgc caaaacggca ttttatgccg
      241 atgttccgaa gaatatgact cagcagaact gtcagtctaa gtacgaaaat tgctactacg
      301 acaatgttga acagagttgg atcccggtcg tttctggatt tttgttaagc cgggttattc
      361 gtaaagatcg cgatgagccg ttcgtctata acagcggcgg atcctcattt gcttcccgcc
      421 ccgtctggcg caacacttcc ggtgattact cctggcgctc cggttctggc aaaaaagagt
      481 cttactcttc aggcggcttt accaccaaaa aagcgtcaac cgtttcgcgc ggcggctatg
      541 gtcgttcttc cagcgcccgt gggcattggg gaggctaatc atgctgagac acaacgttcc
      601 tgtgcggcgg gatctggacc aaattgccgc tgacaacggt ttcgactttc atatcatcga
      661 caatgaaatc tattgggacg agagtcgggc ttaccgtttt actctgcgcc agattgaaga
      721 gcagatcgaa aaaccaactg cggaactgca tcagatgtgc cttgaggtgg tggatcgcgc
      781 ggttaaagac gaagagatcc tgacgcaact ggcgatcccg ccgttgtact gggatgtgat
      841 cgccgaaagc tggcacgcca gcgatccttc gctgtatggc cggatggatt ttgcctggtg
      901 tggcaatgcg ccggtgaagc tgctggagta caacgccgat acgccgactt cgttgtatga
      961 gtcggcttat ttccagtggt tgtggctgga ggatgcccgg cgcagcggca ttattccgcg
     1021 tgatgccgat cagtacaatg ccattcagga acgcctgatt tcacgcttta gcgagcttta
     1081 tagccgggag ccgttttatt tctgctgctg tcaggacacc gatgaagaca ggactaccgt
     1141 gctgtacttg caggactgcg cccagcaggc agggcaggag tcgcggttta tctacattga
     1201 agatctcggt ttgggcgtcg gcggtgtact gaccgatctt gatgataatg tcatccagcg
     1261 tgcatttaag ctgtatccgc tggagtggat gatgcgtgac gataacggtc cgctgctgtg
     1321 caagcgtcgc gagcaatggg tggagccgtt atggaaaagt atcttgagta ataaagggct
     1381 aatgccgctg ctttggcgct tcttccctgg tcatcctaac cttcttgcat cctggtttga
     1441 gggtgaaaaa tcgcagattg ccgctggcga aagctatgtg cgtaaaccga tctactcgcg
     1501 cgaaggcgga aacgtcacca ttttcgacgg tcagaataac gtcgttgacc acgctgatgg
     1561 tgattacgcc gatgaaccga tgatctacca ggcgtttcaa cctctgccgc gatttggcga
     1621 tagctacaca ctcatcgata gctggattgt cgatgatgaa gcgtgcggaa tggggatccg
     1681 tgaagataac acactgatca ccaaagacac ctcacgtttc gttccgcatt acattgctgg
     1741 ataagaatgt tttagcaatc tctttctgtc atgaatccat ggcagtggcc atactaatgg
     1801 tgactgccat tgatggaggg agacacagtg cactggcaaa ctcacaccgt ttttaatcaa
     1861 cctataccat taaataacag caacttatac ctgtctgatg gcgcgctttg cgaagcggta
     1921 acgcgtgaag gtgctggctg ggatagcgat tttctagcca gtattggtca gcagttagga
     1981 acggctgaat cccttgaact ggggcggctg gcgaatgtaa acccgcctga actgttgcgc
     2041 tacgatgccc aggggcggcg tctggacgat gttcgtttcc atcccgcatg gcacctgctg
     2101 atgcaggtgt tatgtaccaa tcgggtgcac aatcttgcct gggaagaaga cgctcgctcc
     2161 ggcgcatttg tggcgcgcgc ggcgcgtttt atgttacatg cgcaggttga ggctgggtcg
     2221 ttatgtccaa taacgatgac ctttgccgcc acgccattgt tgttacagat gttacccacg
     2281 ccgtttcagg actggaccac gccgctgctg agcgatcgct acgattctca cttattgcca
     2341 ggtgggcaaa aacgcggttt gttgattggc atgggaatga cggaaaagca gggtggttcc
     2401 gatgtcatga gcaacaccac ccgtgcagag cgtttggaag atggctctta tcggctggtg
     2461 gggcataaat ggtttttctc ggttccgcaa agcgatgcgc atctggtgct ggcgcagacc
     2521 acgggcggtc tgtcctgttt ttttgtgccg cgctttttgc ctgacgggca acgcaacgcg
     2581 attcgcctcg aacggctgaa agataagctg ggtaatcgct ctaacgccag ttgtgaagtg
     2641 gagtttcagg atgccattgg ctggttattg gggcaggaag gggaaggaat tcgtctgatc
     2701 ctgaaaatgg gggggatgac gcgttttgat tgcgccctgg gtagccatgc catgatgcgc
     2761 cgtgcgtttt cgctggcgat ttatcatgca catcaacgcc atgtttttgg taatccattg
     2821 atccaacagc cccttatgcg tcatgtctta agtcgcatgg cacttcagct tgaagggcaa
     2881 acggcgttgc tgtttcgtct tgcgcgagcg tgggaccggc gcgccgatgc caaagaagcc
     2941 ctgtgggcgc gtttatttac gcctgcggca aaatttgtga tctgcaaacg aggtatgccg
     3001 tttgtggccg aagcgatgga ggtgctgggc ggcattggtt attgcgagga gagcgagctg
     3061 ccgcggcttt accgggagat gccggtaaac agtatttggg aaggttccgg caatattatg
     3121 tgcctggatg tgttgcgcgt tctcaataag caagcgggcg tatacgactt attgtcggaa
     3181 gcatttgtgg aagtgaaagg gcaggatcgc tattttgatc gcgcggttcg tcgtttacag
     3241 cagcagctgc gtaagccagc tgaagaactg gggcgagaga ttactcatca gctattcctg
     3301 ctgggctgcg gtgcgcaaat gttgaaatat gcttctccgc caatggcgca ggcgtggtgt
     3361 caggtgatgt tagatacgcg cggcggcgta cggttgtcag agcagatcca gaatgattta
     3421 ttgctgcggg cgacgggggg agtgtgtttg taaacgtata cgactgatgc gacgctggtt
     3481 tcgattaact caatgaaata tgtgaaaatt gtaggccgga caaggcgctc gcgccgcatc
     3541 cggcattgtt catttgctgt agatgacact catgcataca gtatcgcctg tacgcgccag
     3601 ttatcaggct gctggtcttc gtacatggta ataatgcggt accatgacgc accttgttca
     3661 tcggccttta acgccacgat acgttccacg tcctgcggat cccctgaaat attgctaact
     3721 gagatctgac ctatttcatt caggcccgtc acgcagtcag cactggcgaa ttctgcagac
     3781 tgtgccgtgg cgctcgcagg ataagtcact aattgcagcg agggtgaggc aagtaattgt
     3841 ttcatcgtca gctccatttt tcgtttcccc acatgttgca gggcattcca tcgcggtaat
     3901 aaacgcccag gcttccagcc ggtaatttat tgtgcacagg taaacgcata gggacgcaaa
     3961 gcaatgtaaa gctgactatg taagttgtct gatttattta cggtacaaaa tggcttgtga
     4021 ataccactgt cctgtcacga tggtttcgtc caccatgacg acaacgtaat aatcagcttt
     4081 tgcagcgaca gcttttgctc tgatttctgc taatgcgtca tccggagaac cccgaaccat
     4141 cgtgcttacg ctacctattc gctgtaaccc ttgcgtctgg tcgcggcgaa tctcttgcgg
     4201 atggtccgtt actggcggtg ctggctgtgg cgtaccttgc agtgcgctac aggcacttaa
     4261 catcaacacc aataataaac tggcaaaccg gtaaataacg ctattacgtt tcctgctaac
     4321 catagtgtag tgccttatta atttaacttt gggggaatgt tcccgaattg ttacctggta
     4381 cgcgattttc gaatgaaaac gtgacgaaag cgtaacccac atctcaacat tatgaactaa
     4441 taccgccagc agaaacttgt gctaaggatt tcttcatgtc ataccagaca aaaaggagag
     4501 acagatgatt gaaatagaat cacgcgagct ggcagatatt cccgttcttc atgcttatcc
     4561 tgtcgggcaa aaagataccc cgttaccgtg cgtaattttt tatcacggct ttacttcatc
     4621 cagcctggtg tatagctatt ttgccgttgc gctggcgcag gctggtttgc gggtgatcat
     4681 gccggatgcg cccgatcacg gtagccgttt tagtggcgac gaagcgcggc ggttaaatca
     4741 attctggcaa atcttgctac aaagtatgca ggaattcact actttacgtg cggcaatagc
     4801 cgaagaaaac tggctgcttg atgaccgtct ggcagtcggt ggcgcgtcga tgggcgcgat
     4861 gacggcactg ggaattaccg ctcgccaccc cacggtgaaa tgtaccgcca gcatgatggg
     4921 atcgggctat tttacatcgc tcgcccgttc actgtttcca ccgctgatac ctgaaacggc
     4981 agcacagcag aatgaattca ataacattat cgcgccgctg gcagagtggg aagcgacaaa
     5041 ccacctggaa caacttgggg acagacctct actgctgtgg catggcctcg acgatgacgt
     5101 tgtgcctgcc gacgaatcac tacgtttgca gcaggcctta agcgagacgg gacgggataa
     5161 actgctaacc tgttcatggc agccaggcgt gcgtcaccgc attacgcctg aggcgttaga
     5221 tgctgccgtg acatttttcc gccagcatct ttaaacacgc agaatgctga ccccttgcgc
     5281 ttccagttgt tgcaggattt ccgggttagc atttttgccg gtgataagca tatcgatttg
     5341 atcggcacgg ctaaaaagca ttcccgcgcg ttcgccaatc ttactgctat caaccagtac
     5401 caccagtttc cctaccacgc tcagcatctt ctgctctgcc attgctgtca gcatatcggt
     5461 tttatacaac ccttctgcgg tcagcccttt tccgctggta aacatccagt gcccggcata
     5521 gagactgttt tcgctgccct gcgggcttaa agtgatggac tgacttttgt tgtactgtcc
     5581 acccataatg atcacgctgt catgttcttg atcgatcagg taatttgcca gcggtagata
     5641 attagtgatg atttgcactg gcttgccaca catttcccgc ccaagcagaa acgcggtgga
     5701 gccgcagttg atgactacgc tttcgcccgg attaaccagc tgcgaggccg ctttagcaat
     5761 acgtactttt tcatcgtgat tctgcgcctg atgcagattc atcggcgtcc agcgcgggcg
     5821 ctgttgggta atagcttctg cgccattgcg cacctttttc agtttgccgc gttcgtcaag
     5881 tttattgata tcgcgtcgcg cagtggcagg cgaaattccc agacgctcaa cgactttctc
     5941 aacggtcaca aagcccaatt gtgcgagcat ttccaggagg atttgatgtc tttgtgcttc
     6001 agtcatgagc tattccgata aaatttgatt tttttagatg atatttgaaa tagctaggaa
     6061 atactacgcc ggatagcgcg aacttctcca cgctatccgg tataaacgac agattacagg
     6121 aatgacttga acggcagatc cggttcaata gtgaagcaat catcgaaacc gcgcggatag
     6181 tggtactcga agttgtcttt atccagcggc caggtaaatt tgccgccaac ctgccagata
     6241 aacggcttga agccatactt cagacgatct tttttcatct cccacagcac gcggatctct
     6301 tgcggatcgg cctggaagtt tgaccagatg tcgtggtgga acgggataac cactttcgca
     6361 ttcagggctt cacccatacg cagcatatcg gcgctggtca ttttgtcggt aataccgcgc
     6421 gggttctcac catacgatcc taacgccaca tcgatctgat gttcgttacc gtgcttcgca
     6481 taatagttag agtagtggga atcgccactg tgatataggg aaccgccagg ggttttgaac
     6541 aggtagttta ctgcgcgatc gtccatgcca tctggcagta cgccagccgc tttttgatcg
     6601 gcaggcaggg tgatcagtgc agtacggtcg aaagcgtcaa gcgcatgaat ttcaatgtct
     6661 ttcactttta caacatcgcc cggtttgacc acgatgcaac gctctttcgg tacgccccag
     6721 ccaatccaca aatcaacgca ggttttcggt ccaatgaatg gtacgtcatc agcacagttc
     6781 tgcatcacgg cagcagcgac gttaacgtcg atatgatcgt tgtgatcgtg agtcgccagt
     6841 accgcgtcga tctggcgaat cgcaaacgga tcaagaacaa acggggtggt gcgtaggttt
     6901 ggctgcagtt ttttcacgcc agccatgcgc tgcatctggt gaccctgttt cattaacggg
     6961 ttaccgtgac tttgtttgcc agtgccgcac cagaaatcaa cgcaaacgtt ggtgccacct
     7021 tctgatttca accagatccc ggtgcagcct agccaccaca tcgcaaatgt gccaggggcg
     7081 acctgctctt gttcaatttc ttcattcagc cagctacccc actccgggaa agtgctcagg
     7141 atccaggatt ctcgggtgat acttttcact ttactcatcg ccatttacct tcatgatagt
     7201 tcaattcgaa tcaatatgtg attggttttg attaatcctg acactatttt ttcaggaagg
     7261 caatgaccat tttttgactt tttgccaggg aagttgttgt tgattttgag tgtggaaaga
     7321 tttaatggaa tgtgtagtta aattaactaa atgaatttaa ataaataatt gtttcgttgt
     7381 gtgaatccca ctctatccgt gtggaattat ttgcgggtcg cgtcacattt aatcataaat
     7441 aatcttgttg tgattacttt tgaaaattag agtgagtgca caacattccg ggtgtgcgga
     7501 atacccggtt acctcttctt caggagatcg ttatggagat cctctacaac atctttaccg
     7561 tgttttttaa ccaggtcatg accaatgccc cgttgttgct gggtattgtg acctgtctgg
     7621 gctacatcct actgcgcaaa agtgtcagcg ttattattaa aggcacgatt aaaaccataa
     7681 ttggtttcat gttgttgcag gcagggtccg gcatcctcac cagcaccttc aaaccggtgg
     7741 tggcgaaaat gtccgaagtc tacggcatta acggcgcaat ttccgatact tacgcgtcga
     7801 tgatggcaac catcgaccgc atgggcgatg cctatagctg ggtaggttac gcggtgttgt
     7861 tagcgctggc gctgaacatc tgttacgtgc tgctgcgtcg cattaccggt attcgtacca
     7921 tcatgctgac tggtcatatc atgttccagc aggcggggct gattgccgtt acgttgttta
     7981 ttttcggcta ctccatgtgg acaaccatta tctgcacggc gattctggtt tcgctctact
     8041 ggggcattac ctccaacatg atgtacaagc cgactcagga agtgacggac ggctgcggtt
     8101 tctccatcgg tcaccagcaa cagtttgcat catggactgc ctataaagtt gcgccgttcc
     8161 tcggcaaaaa agaggaaagc gttgaagacc tcaaactgcc aggctggctg aatattttcc
     8221 acgacaacat cgtctccacg gcgattgtca tgaccatctt ctttggtgcc attctgctct
     8281 ccttcggtat cgacaccgtg caggcgatgg caggcaaagt gcactggacg gtctatatcc
     8341 tgcaaaccgg tttttccttc gcggtggcga tcttcatcat cactcagggt gtgcgcatgt
     8401 ttgtggcgga actctctgaa gcgtttaacg gtatctccca gcgcctgatc cctggtgcgg
     8461 ttctggcgat tgactgtgcg gctatttata gcttcgcgcc gaacgccgtg gtctggggct
     8521 ttatgtgggg caccatcggt cagctgattg cggttggcat cctggtcgcc tgcggttcct
     8581 cgatcctgat tatcccaggc tttatcccga tgttcttctc caacgccacc atcggcgtgt
     8641 tcgctaacca cttcggcggc tggcgtgcgg cgctgaagat ttgtctggtg atggggatga
     8701 tcgaaatctt tggttgcgtc tgggcggtga aacttaccgg tatgagtgcc tggatgggga
     8761 tggcggactg gtcgattctg gcaccgccga tgatgcaggg cttcttctcc atcggtatcg
     8821 cctttatggc cgtcatcatt gtaattgcac tggcttatat gttcttcgct ggccgcgcac
     8881 tgcgcgcaga agaagatgca gaaaaacaac tggcagaaca gtctgcttaa taaggagttt
     8941 tgattatgac cgtacgtatt ctggctgtgt gtggcaacgg acaaggcagt tccatgatca
     9001 tgaggatgaa agtggaccag tttttaaccc aatcaaacat tgaccacacg gtaaatagct
     9061 gcgcggttgg cgaatacaaa agcgagttga gcggcgcgga tatcatcatc gcttctacgc
     9121 acattgcggg cgaaatcacc gtgaccggca acaaatacgt ggttggcgtg cgcaacatgc
     9181 tctctcctgc cgactttggc ccgaaactgc tggaagtgat caaagcgcat ttcccgcagg
     9241 atgtgaagta aggacgcgcc atgaaattac gtgattcgct ggcggagaat aaatccatcc
     9301 gcctgcaggc tgaagctgag acatggcagg acgcagtaaa aatcggcgtt gacctgctgg
     9361 tggcggcgga tgttgtcgag ccgcgttact accaggcgat tctggatgcg gttgaacagc
     9421 acggtccgta ctttgtgctc gcaccaggcc tggcaatgcc gcacggtcgc ccggaagagg
     9481 gcgttaagaa aaccggtttt gcgctggtga cgctgaaaaa gccgctggag ttcaaccacg
     9541 aagataacga cccggtggat atcctcatca ccatggcggc ggtcgatgcc aacacgcacc
     9601 aggaagtggg catcatgcag atcgtcaacc tgtttgaaga tgaagaaaat ttcgaccgtt
     9661 tacgtgcctg ccgtaccgag caggaagtac tggatctcat tgaccgcacc aacgcggcag
     9721 cttaagaagg aattgaagat gtcattaccg atgttgcaag tcgcgctgga caaccagact
     9781 atggatagcg cctacgaaac cactcgcctg attgccgaag aagtcgacat tatcgaagtg
     9841 ggtaccattc tgtgcgtggg cgaaggcgtg cgtgcggttc gcgatctgaa agcgctctac
     9901 ccgcacaaaa tcgtgctggc agacgccaaa attgccgatg cgggcaaaat tctttctcgt
     9961 atgtgcttcg aagccaacgc tgactgggtg accgtgattt gttgtgcgga tatcaacacc
    10021 gccaaaggtg cgctggacgt agcaaaagag tttaacggcg acgtgcagat cgaactgacc
    10081 ggttactgga cctgggaaca ggcgcaacag tggcgcgacg caggtattca gcaggtggtt
    10141 tatcaccgca gccgtgacgc gcaggccgct ggcgtggcgt ggggtgaagc cgatatcact
    10201 gcgatcaaac gtctttccga tatgggcttc aaagtcaccg tcaccggtgg cctggcgctg
    10261 gaagatctgc cgctgttcaa aggcattcca attcatgtgt ttatcgccgg tcgcagcatc
    10321 cgtgatgccg cttctccggt tgaagccgcg cgtcagttca aacgttccat cgccgaactg
    10381 tggggctaag gagcggatat gttgtccaaa caaatcccgc ttggcatcta tgaaaaagcg
    10441 ctccccgccg gggagtgctg gctggaacgc ctgcgactgg caaaaacgtt gggcttcgat
    10501 tttgtcgaga tgtcggtaga tgaaactgac gagcgcctgt cgcgcctcga ctggagccgc
    10561 gagcagcgtc tggcgctggt gaatgcgatt gttgaaaccg gcgtgcgtgt gccgtccatg
    10621 tgcctttctg cccatcgtcg tttcccgttg ggcagcgaag atgacgcggt gcgggcgcag
    10681 gggctggaga ttatgcgtaa agccatccag ttcgcccagg acgttggcat tcgcgtgatc
    10741 cagctggcgg gctatgacgt ttactatcag gaagccaata acgaaacgcg tcgtcgtttc
    10801 cgtgacggcc tgaaagagag cgttgagatg gcgagccgcg cgcaggtgac gctggcgatg
    10861 gagatcatgg attatccgtt gatgaactcc atcagcaagg cgctgggtta cgcgcactat
    10921 ctcaacaacc cgtggttcca gctctacccg gatatcggca acctgtcggc gtgggacaac
    10981 gatgtgcaga tggagttgca ggccggaatc gggcatatcg tcgcggtgca tgtgaaagac
    11041 accaaaccgg gcgtcttcaa aaacgtgcca tttggcgaag gtgtggtgga tttcgaacgt
    11101 tgcttcgaaa cgctcaaaca gagtggctat tgcgggccgt acctgattga gatgtggagc
    11161 gaaacggcgg aagacccggc ggcagaagtg gtgaaagcgc gtgattgggt gaaagcgcgc
    11221 atggccaaag caggcacggt ggaggcggca taatgcaaaa gctaaaacag caggtgtttg
    11281 aagccaacat ggatctgccg cgctatgggc tggtgacctt tacctggggc aacgtcagcg
    11341 ctatcgaccg cgaacgcggg ctggtggtga tcaagcccag cggcgttgcc tacgaaacca
    11401 tgaaagcaga cgatatggtg gtggtcgata tgagcggcaa ggtggtggaa ggggagtatc
    11461 gcccgtcttc cgacaccgcg acgcatctcg aactctaccg tcgttacccg tcgcttggcg
    11521 gcattgtcca tacccactcc actcatgcta ctgcatgggc gcaggcgggg ctggcgatcc
    11581 cggcgttagg cactacgcac gccgactact tctttggcga catcccgtgt acgcgcggat
    11641 taagcgaaga agaggtgcag ggcgagtatg aactgaacac cggcaaagtg attatcgaaa
    11701 cgctgggtga cgccgagccg ctgcatacgc cggggattgt ggtgtatcag cacggtccgt
    11761 tcgcctgggg gaaagatgcc cacgacgcgg tgcataacgc ggtggtgatg gaagaagtgg
    11821 cgaaaatggc gtggattgcg cgcagtatta acccacaact gaatcacatc gacagcttcc
    11881 tcatgaataa acacttcatg cgtaagcacg gtcctaacgc ctattacggg cagaagtaag
    11941 cagtgcgccc cggaattatt acctcctgcg ggatgtaatt ggtctgattc cggggccgct
    12001 tcagttaaaa aatcgtataa acaacaggtg tttttatggc tatcaatgga ttgtttgtca
    12061 gaacggatcc gcaacgtcgc cgctatggcg ttgcgttact tgtgggtgta ctttcaggaa
    12121 ttatttcagc atttgttaaa tggggagcgg aagtcccctt gccaccgcgc acattttccg
    12181 gtggtcgcga tgaatttaac ccgccgtata tctttctacg ggattattta ggtatcgacc
    12241 caacccaaac tgtgtataca ttctcggaac atattatcaa cccggtgatg gtgacgcata
    12301 tcatcttctc actggttttt gcgatagggt attgtattgt cgcggaaatt ttcccgaaag
    12361 tgaaattatg gcaggggata ttagcagggc ttattgtgac agtggcagtc cacggtatat
    12421 tttgcccggc gctgaattta acgccgccgc tcactcaatt gccttttgac gaatatgcct
    12481 ctgaaatcct ggggcatatt ttctggttct ggataataga aattatgcgc cgcgacttac
    12541 gtaatcgcat tactcatgag ccagatccgg aagtcgcaat ccggtgaata accgcataaa
    12601 aaagccccct gcgaaggggg caaagcaaac tatggcaatg tttcgttggt tatacctggt
    12661 gctagcgata aatatccgcg ctggcgtgca tattgccgtt actccccggt tcccgcatca
    12721 gaattacgtg gtagtacgtt gcgccctgcg catctgtctc ttcatttaat gcctgacgcg
    12781 cttcgctttc agtggcgaaa ttatgattga tataaatcac gcctaagctt tgcacatcat
    12841 ccatgtttct ggcctggtgg ttattaattt caatggctgc ccatgtattt gcacttagca
    12901 aaagcacagc cagaagggct aaaactcgac tgaacataga tacctcctcg acggctgact
    12961 ttgtgtgctc tcctctgtga tgatcttctg atttaatttt aatcaatgat aaagaagttg
    13021 atggtgacca tttctgatgc agttgttcaa aaaaacacca tgatgaagtg tgatgaactc
    13081 caaatcagcg gtttagaggt taattgcgaa aggggagatt tatttcggct ctgcccttga
    13141 gtttagcggg gcatacaagt actataacgc gtcatttttc agccgacctt taacacgttc
    13201 cttgcctccc cgggattcgg ctgacccaga caggaggctg aataatccgt aaggagcaat
    13261 tcgatgcgtc attacgaaat cgtttttatg gtccatcctg accagagcga acaggttccg
    13321 ggcatgatcg agcgctacac tgctgccatc actggtgcag aaggcaagat ccaccgtctg
    13381 gaagactggg gccgccgtca gctggcttac ccgatcaaca aactgcacaa agcacactac
    13441 gttctgatga acgttgaagc tccgcaggaa gtgatcgatg agctggaaac taccttccgc
    13501 ttcaacgatg ccgttatccg cagcatggtt atgcgtacca agcacgctgt taccgaagca
    13561 tctccgatgg ttaaagcgaa agacgagcgc cgtgagcgtc gcgatgattt cgcaaacgaa
    13621 accgctgatg atgctgaagc tggggattct gaagagtaat ttctgatgac caaccgtctg
    13681 gtgttgtccg gcaccgtgtg caggactccc cttcgaaagg tcagtccatc aggaattcct
    13741 cactgccagt tcgtgcttga gcatcgttct gtgcaggagg aagccggttt tcaccggcag
    13801 gcgtggtgcc aaatgcccgt tattgttagc ggacacgaaa accaggccat tactcacagt
    13861 ataacggtcg gcagtcgcat aaccgttcag gggttcattt catgccacaa ggcaaagaac
    13921 ggactgagca aaatggtttt gcatgccgag cagattgaat tgatagattc tggagactag
    13981 ccatatggca cgttatttcc gtcgtcgcaa gttctgccgt ttcaccgcgg aaggcgttca
    14041 agagatcgac tataaagata tcgctacgct gaaaaactac atcaccgaaa gcggtaagat
    14101 tgtcccaagc cgtatcaccg gtacccgtgc aaaataccag cgtcagctgg ctcgcgctat
    14161 caaacgcgct cgctacctgt ccctgctgcc gtacactgat cgccatcagt aatcggtcac
    14221 ggtccattaa tacgactttg agaggataag gtaatgcaag ttattctgct tgataaagta
    14281 gcaaacctgg gtagcctggg tgatcaggta aacgttaaag cgggctatgc tcgtaacttc
    14341 ctggtaccgc agggtaaagc tgttccagct accaagaaaa acattgaatt cttcgaagca
    14401 cgtcgcgctg aactggaagc taaactggct gaagttctgg cagctgctaa tgctcgcgct
    14461 gagaaaatca atgcactgga aactgttacc atcgcgtcta aagctggcga cgaaggtaaa
    14521 ctgttcggtt ccatcggtac tcgcgacatc gctgacgctg taactgcagc tggcgttgaa
    14581 gtggctaaga gcgaagttcg tctgccgaac ggcgttctgc gtaccactgg cgagcacgaa
    14641 gtgagcttcc aggttcacag cgaagtattc gcgaaagtga tcgtaaacgt agtagctgaa
    14701 taattcgtta ttcaacgaga cgtaaaaacg ccgaccagtg gtcggcgttt tgcttttgta
    14761 gcgagcactt gcagatatcg ataagggcca caataagcag ccctacgaag ataattactt
    14821 agctgcctga atatcagcca gcattggggc aagggctggg ttgtccttag cgaagtttgc
    14881 gtacattggc tgtactttct cgataaacgg agctttatct acctggtaga ctttcacgcc
    14941 cattttatcc agttttgcaa attcttgttt ctcttcctcg ctccacaagt ctttcatgta
    15001 gctcattgat tctttagctg cttttaatat aactgcctga ttttccgggc taagtttatc
    15061 aaacgcagca ttgctcatca gaaccacatc gggaaccata gtgtgttcgt caaggctgaa
    15121 ggattttgcc acttcaccgt gacgcatggt ggtcagcgcc atcacgctgt tttctgccat
    15181 atcgaccaca ccctgctgta aggctgtata caactcgccg taatccagtg gcgtcgggtt
    15241 accgcccatg actttgatca tttcaactgc gccagggctt ggctggacac ggactttcat
    15301 gcctttcaga tcgtctggtt tcagtactgg tttattgcca tagaaactgc gggcgcctcc
    15361 atcataaaaa gtcagcccga aataaccttt gcttttcgtt gagtcgagga tttttctccc
    15421 gatatcgccc tgcatgacct gataataatg atcacgatcg cggaacaaat aaggcaggct
    15481 aaataattta taggaatttt caaatgattc catttctgcc gcattggttt ttaccagtgg
    15541 aatagcgcca gaacgaatca gctccattgt ttctcgttga gtacccaatg ttccatttgg
    15601 ataaatacga atagtaatat caccgttaga gtactctttg ttctttttcg caaagaactc
    15661 catcgcttta tgaacaggat gagacttatc ctgattatga ctcagtttta atgtcactgc
    15721 ccaggactgg cttgcggata acataagaac agcaccgata atgccagcgg atacttttat
    15781 tttcatagat gttccttttc taataaatat ggcggcaggt gccgccagtg aatgcgcatc
    15841 agtagcccag tgcttttggc aatgccatac tgatttgcgg aatataggtc accattgcca
    15901 gcaccaatac catcacggca tagaatggca gcataggttt gataatttta ttaatcggta
    15961 atttagatac actacaaccg acaaataaca cactgcctac aggcggagta caaataccga
    16021 tacacatgtt cagtaccatg acaataccaa agtgaatcgg gtccatgccc agttcggtga
    16081 caacaggtaa aaatatcggc gtaaaaatca gtaccgccgg tgtcatatcc ataaaagtgc
    16141 cgataatcaa caggataata ttgataataa gcaggattat tattgggttg tcagaaatag
    16201 tgttaagcaa atcgagaatc aggaagggaa cgtcagcgtt tgacattgcc ca
//
LOCUS       DAKISA010000099        15272 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.099, whole genome shotgun sequence.
ACCESSION   DAKISA010000099 DAKISA010000000
VERSION     DAKISA010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15272
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.099"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(358..1133)
                     /locus_tag="OVW45_003293"
                     /pseudo
     CDS             complement(358..1133)
                     /locus_tag="OVW45_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1178..1612)
                     /locus_tag="OVW45_003294"
     CDS             complement(1178..1612)
                     /locus_tag="OVW45_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HCV5527666.1"
                     /translation="MYGTCETLCRELAAQYPGDTPLMLVIWSPEEIQALADGMDISLS
                     DHEIRTVLARLEDIPEDQRTESGISSGVAMEIINNVRENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDHGLAVPECVTRRQAVVNQARALLKNNTHEND"
     gene            complement(1626..1847)
                     /locus_tag="OVW45_003295"
     CDS             complement(1626..1847)
                     /locus_tag="OVW45_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5527667.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LTRQTLYRANRLFMEAYTEILELDSCFKD"
     gene            complement(1848..2531)
                     /locus_tag="OVW45_003296"
     CDS             complement(1848..2531)
                     /locus_tag="OVW45_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCV5527668.1"
                     /translation="MSRFILGDCVRVMATFPGNAVDFILTDPPYLVGFRDRSGRTIVG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKTYT
                     SKAAYVGYRHECAYILAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DNWFEPEAA"
     gene            complement(2608..2901)
                     /locus_tag="OVW45_003297"
     CDS             complement(2608..2901)
                     /locus_tag="OVW45_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5527669.1"
                     /translation="MFLGFVWRVTKDVAGLLFLLRRLTRRGRNAACGQCPSSGRARDG
                     RFYRSRLALVTVYASREPFSDERPSSRFRGIALPSKRRRLRYSTVGLTRYRTR"
     gene            3049..4011
                     /gene="parM/stbA"
                     /locus_tag="OVW45_003298"
     CDS             3049..4011
                     /gene="parM/stbA"
                     /locus_tag="OVW45_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HCV5527670.1"
                     /translation="MLVFIDDGSTNIKLQWQESDGTIKQHISPNSFKREWAVSFGDKK
                     VFNYTLNGEQYSFDPISPDAVVTTNIAWQYSDVNVVAVHHALLTSGLPVSEVDIVCTL
                     PLTEYYDRNNQPNTENIERKKANFRKKIILNGGDTFTIKDVKVMPESIPAGYEALQEL
                     DELDSLLIIDLGGTTLDISQVMGKLSGISKIYGDSSLGVSLVTSAVKDALSLARTKGS
                     SYLADDIIIHRKDNNYLKQRINDENKISIVTEAMNEALRKLEQRVLNTLNEFSGYTHV
                     MVIGGGAELICDAVKKHTQIRDERFFKTNNSQYDLVNGMYLIGN"
     gene            4014..4364
                     /locus_tag="OVW45_003299"
     CDS             4014..4364
                     /locus_tag="OVW45_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCV5527671.1"
                     /translation="MDKRRTIAFKLNPDVNQTDKIVCDTLDSIPQGERSRLNRAALTA
                     GLALYRQDPRAPFLLCELLTKETTFSDIVNILRSLFPKEMADFNSSIVTQSSSQQEQK
                     SDEETKKNAMKLIN"
     gene            4622..4864
                     /locus_tag="OVW45_003300"
                     /pseudo
     CDS             4622..4864
                     /locus_tag="OVW45_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            4907..5065
                     /locus_tag="OVW45_003301"
                     /pseudo
     CDS             4907..5065
                     /locus_tag="OVW45_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5215..6213
                     /locus_tag="OVW45_003302"
     CDS             5215..6213
                     /locus_tag="OVW45_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCV5527672.1"
                     /translation="MKKVLCALGLMFTAVSSALATTYPLTIENCGYQETFTRPPERVV
                     ALGQNTVEILLLLGLQKQVVASAFWPTSVLPQLAEQNAKIKTLTVEIPSLESVLAQNP
                     DFVPAQLPLLLGPESKVARREDLATVGVNSYVSPGMCATKKATGDMYGSRQKLWDMTW
                     LYQEISDFSRIFNVEDRGQALIADFKKREADLRQEFGKSKKDLSFVFWFSSASPSADA
                     YVGGKNSASGFIASVLGGHNAITSETEWPTVSWESIIAANPDVIVVASLDRNRWALDK
                     AEEKIKFLKSDPAVSQLEAVKKGHIVVMDGQAMNPTIRTLYGAEQVGEQLRKMGLN"
     gene            6213..7250
                     /locus_tag="OVW45_003303"
     CDS             6213..7250
                     /locus_tag="OVW45_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCV5527673.1"
                     /translation="MSVAAIETRRSILLTGWCVLAAIVLALVIAVGVSVGELAIPLQN
                     VFYAISNRTGLTVEPLNRIYESVIWDFRLSRALVAACCGAGLAICGVVLQSLLKNALA
                     EPYVLGVSAGASTGAVSIVVLGLGAGAISLSAGAFAGAFAAFAFVALLTNGARGGNER
                     TILAGVAASQLFNAITAYTISTSASAQQARDVMFWLLGSFSGVRWPEFQLVIVVVLAG
                     LAVCLWYARALDAFTFGDDAAASLGIAVPRVRLILFTTAALITATIVSMAGSIGFVGL
                     VVPHVMRFFFGPLHRTLLIASALAGAILMVLADIASRLLIAPQSLPVGVVTALVGVPF
                     FAVIIYRSRNK"
     gene            7250..8011
                     /locus_tag="OVW45_003304"
     CDS             7250..8011
                     /locus_tag="OVW45_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCV5527674.1"
                     /translation="MSICAENITWKAGKKVIVNNVSLRVPRGETVGLLGPNGCGKSSL
                     LRVLAGLRRPDAGRVTLDGQDIARMAKKQLARRVACVEQHGMTEANMRVRDVVRLGRI
                     PHHSPFSNWSAQDDEAIAAALQRVAMLEKSEQGWLSLSGGERQRVHIARALAQSPSEI
                     LLDEPTNHLDIHHQMQLMQLISELPVTSIVAIHDLNHAAMFCDSLIVMQQGQILASGT
                     PEEILSEALLWDVFRVKTKIEISPYHGKKHIHFIV"
     gene            8068..9255
                     /locus_tag="OVW45_003305"
     CDS             8068..9255
                     /locus_tag="OVW45_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCV5527675.1"
                     /translation="MLLGSQFVFNIGFYAVVPFLALFLRDDMLLSGGLIGLILGLRTF
                     SQQGMFILGGTLADRYGAKAIILAGCVVRVAGFLLLACGASLWPIILGACLTGVGGAL
                     FSPSIEALLARAGTHSQANGKRSRAEWFALFAVCGELGAVIGPVAGGVLSGIGFRHIA
                     LAGAGIFLLALAVLFFGLPADGHTTTTRRRVPWWTPLRQPRFVAFILAYSSWLLSYNQ
                     LYLALPVEIQRSGGREQDLAPLFMLASLLIITLQLPLARFARRMGAVRILPVGFLLLS
                     ASFASVALFAAAPPAESWLRLMPAAGFVTLLTLGQMLLVPAAKDLIPLFAEESTLGAH
                     YGALATAGGCAVLAGNLLLGHLLDQALIPSPQAVYPWLLLALFPLCSAVALRAICRPL
                     AAT"
     gene            complement(9333..9608)
                     /locus_tag="OVW45_003306"
     CDS             complement(9333..9608)
                     /locus_tag="OVW45_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCV5527676.1"
                     /translation="MKYSKPISSPTCFGERISTELEFVRKICRAEGETEADDDKLVDE
                     FRRLAEHPDGSDLIYYPRDDREDSPEGIVKEVKEWRAKNGKPGFKQG"
     gene            complement(9966..10265)
                     /locus_tag="OVW45_003307"
     CDS             complement(9966..10265)
                     /locus_tag="OVW45_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCV5527677.1"
                     /translation="MSYEIEWRPGAIEDITALFEYLAENASLWDARNVTERLITATDR
                     LADYPRLYETDERYGEGVRRISLLGQHVLYEVNDKARRVNILAVVGQRQNPRFIL"
     gene            complement(10255..10518)
                     /locus_tag="OVW45_003308"
     CDS             complement(10255..10518)
                     /locus_tag="OVW45_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HCV5527678.1"
                     /translation="MNSTTHYENANFLRELAESLPRIFPEGSTDKSALLQRLANEELA
                     RAEYDEQIRAKVAAARADKRPGMSSVQLRQQLQGRYQELRNEL"
     gene            complement(10625..10885)
                     /locus_tag="OVW45_003309"
     CDS             complement(10625..10885)
                     /locus_tag="OVW45_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5527679.1"
                     /translation="MAENGYGLAGQGMGKVKSINPYRLTPGFGGFTPVSHVTTACRLT
                     CRWRGISICWWLCLKGYILCIKPDNTQIAKQMSIMHPVIACL"
     gene            complement(11587..12444)
                     /gene="repA"
                     /locus_tag="OVW45_003310"
     CDS             complement(11587..12444)
                     /gene="repA"
                     /locus_tag="OVW45_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCV5527680.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESDAGTLSITRATRALTFLAELGLITYQTEYDPLIGCNIPTDITFTPALFAALDV
                     SEEAVASARRSRVEWENRQRKKQGLDALGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDANRERQDIVSLVKRQLTREISEGRFTANREAVKREVERRVKERMILSRNRN
                     YSRLVTASP"
     gene            complement(12437..12511)
                     /gene="tap"
                     /locus_tag="OVW45_003311"
     CDS             complement(12437..12511)
                     /gene="tap"
                     /locus_tag="OVW45_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCV5527681.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            complement(12756..13004)
                     /gene="repA"
                     /locus_tag="OVW45_003312"
     CDS             complement(12756..13004)
                     /gene="repA"
                     /locus_tag="OVW45_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCV5527682.1"
                     /translation="MSQTENAVTSSSGTKRAYRKGNPLTLAERQQASLARKRATHKEL
                     RVFIPAALKAQLQVMCEAEGVTQAEMIAELIKQKSAFS"
     gene            complement(13244..13834)
                     /locus_tag="OVW45_003313"
     CDS             complement(13244..13834)
                     /locus_tag="OVW45_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCV5527683.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVERRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            complement(13872..14081)
                     /gene="hha"
                     /locus_tag="OVW45_003314"
     CDS             complement(13872..14081)
                     /gene="hha"
                     /locus_tag="OVW45_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCV5527684.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLLNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            complement(14127..14588)
                     /locus_tag="OVW45_003315"
     CDS             complement(14127..14588)
                     /locus_tag="OVW45_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCV5527685.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            complement(14833..15045)
                     /locus_tag="OVW45_003316"
     CDS             complement(14833..15045)
                     /locus_tag="OVW45_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5527686.1"
                     /translation="MNGFRNSSRNGQVWRWQRAGSRAVILEVSGRWMEAAEAWRRAAG
                     VAPRTDWQQFARKRAEQCHRRCRGRV"
     gene            complement(15176..>15272)
                     /locus_tag="OVW45_003317"
     CDS             complement(15176..>15272)
                     /locus_tag="OVW45_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCV5527687.1"
                     /translation="QEEEVYAAERLDKIRRQNRIKAELQAVLDEK"
BASE COUNT         3509 a         4170 c         3941 g         3652 t
ORIGIN      
        1 tttgccgggc ctgcagggtt gcaagggcgc aagggaagcc gcagcctgac gcacacggct
       61 ggcgggagtt ggagggtgag accgcgaaat ctgagcgggt gaacgaccgg aaaatcacgg
      121 cagacgggaa ggagggaccg ggtgaccgca gcggttcacc ttaccttgca acccgcccgc
      181 agaagcccgg ctacgatgga ggcacggcac agcccgcaca cgacgctgag gccacataac
      241 tgaaaaccca caaggccatg ccccgcatgg ccgggtaaag cggagcctgc ggcgacatcc
      301 ttttgctctt tccggggacc ggctggaaaa ggcggagatt tttccccgcc agcgtcatca
      361 tttccggatt ttcgccagtg catccgccat gctgcggatt tcatcccgtg cctccagaac
      421 aacctcccgc agaatatccc ggtcaaacca gcgacgggac ggttcatccg gtatacgcag
      481 cacatcacag accagaccag accactggac aggatatgct ccacacactc actgaccaga
      541 tacatttcca ccgccgaaaa acgtgctccc cggttcagga tggcatcgag caccacgggc
      601 agattttcgt catcttcatc aagccagcag gggtaatcgc tttccttcac aaccagacga
      661 aagcggcgcg tctggcgggt cagcaacgtc tgaccggtgg tggtcagcgc cgcacggata
      721 cgccgcgcat cttctgccac ccggcaactg acactgcata ctgcatcgcg caccgcatca
      781 agaaccgccc tgatttcttc ctccgtcagc cattcaccgg catacgggaa agacacctgc
      841 gttccgtctg ccggaagtac cggcagcgac tgccactgac tgccggacag aacaagttcc
      901 gctccggttc cggtcagttt ctgaatggca gaggccagga gcggcggcag tacgccgccg
      961 tgacggttga taagtaacgt cgccagccgg ggcgcatcca gccgcacgta aatgctgatc
     1021 cagcccggat agtgcggatt cagttgttca atatccgtct cttccggcca accctgcgcc
     1081 agcagcaggt tacgggcgtg gcgataaacc agtgcattca gcgttttgtt catgattaat
     1141 ggtctccctg aatcgccgcc cgaaagcggc gataacatca gtcgttttcg tgtgtgttgt
     1201 ttttcagcag ggcgcgggcc tgattaacca ccgcctgacg gcgggtgacg cattccggga
     1261 cggcgaggcc atgatcccgg gcggcccatt cacgtttcca cagtgcctgt tcggcggtct
     1321 gaatcaggga cgccagcagt tcagccggga cggtcacctg gcggttttct ctcacgttat
     1381 tgatgatctc catcgccacg ccggaagaaa taccggattc agtccgctgg tcttccggga
     1441 tgtcctccag gcgcgccagg acagtcctga tttcatgatc ggacagggaa atatccattc
     1501 cgtcagcgag ggcctgaatt tcttccgggg accagataac cagcatcagc ggcgtgtctc
     1561 ccggatactg cgctgccagc tcccggcata gcgtttcgca tgttccgtac attctcgtct
     1621 ccttctcaat ctttgaagca ggaatccagt tccagaattt cggtatacgc ctccatgaac
     1681 aggcgattgg cgcgatacag cgtctggcgt gtcaggcgtt caacaagcac atcggaatca
     1741 aagcgacagc gatgctccat atcgttcagc ctggcccgaa ggtcacacat ggtgttggcc
     1801 acctccgcca cgtcggcgcg aagtttttcg tgtccggcat agttcattta cgcagcctcc
     1861 ggttcaaacc agttatcatt cgcggccccc tgctgcatgg cccgttgcac ggcggccagt
     1921 cgttgctgtc cggcacggtg atactgttca agcagctcga taccgatata ccggcgtccg
     1981 gactgaaggg cggccacaca ggttgagccg ctgcctgcaa acgggtccag cacaatcgcg
     2041 ttcgggtgtg tgaagctctc aatcagcggt tgcaggctgg taacgggctt ttcagtcggg
     2101 tgatgacgat tgcctgaata tttccagccc agcacgtcgg gcaatggctt ctgaggcagc
     2161 gccggacgtc cttttgccag gatgtaggcg cattcgtggc gatagcccac atatgcggcc
     2221 ttcgatgtgt aggttttggt aaacaccagg tggccaacca cgctgaatcc cgcatttttc
     2281 caggcggcca taaagcgatc gacgcggttc cagccataga agctcaccat taacgcgtct
     2341 tttttgagta cgcggtacat ttcattacag gctggttgca gccattcatc ggttttatcg
     2401 ccgacgatgg tgcgcccgga acggtcacgg aaaccgacga gatacggcgg gtcggtgagg
     2461 atgaaatcga cagcattgcc cggaaacgtg gccataacac gcacgcagtc acccaggata
     2521 aaacgggaca taacagcact ccttctctta cagagggcac cgggagggca cccctgtggt
     2581 gcccgttccc cgctgcctgt gggctgttca cctcgtccgg taacgggtca ggccgaccgt
     2641 ggaataccgc agccgtcgtc tttttgacgg caaggctatg ccgcgaaagc ggcttgaagg
     2701 gcgttcatca gaaaagggct cccggctggc gtacactgtc accaacgcca gccgggagcg
     2761 gtaaaagcgc ccgtcccgtg cccggccgga cgagggacac tgaccacagg cagcgttcct
     2821 gccgcgccgc gtcagccggc gcagtaaaaa gaggagcccg gcgacatctt ttgtcacccg
     2881 ccaaacaaaa cccaaaaaca acccacaccc aacccaataa aacaccaaaa caagacaaat
     2941 aatcattgat tgatggttaa aatggggtaa acttgacaaa taaacccact taaaacccaa
     3001 aacataccca aatacacacc aaaaaaaaca ccataaggag ttttataaat gttggtattc
     3061 attgatgacg gttcaacaaa catcaaacta cagtggcagg aaagcgacgg aacaattaaa
     3121 cagcacatta gcccgaacag cttcaaacgc gagtgggcag tctcttttgg tgataaaaag
     3181 gtctttaact acacactgaa cggcgaacag tattcatttg atccaatcag cccggatgct
     3241 gtagtcacaa ccaatatcgc atggcaatac agcgacgtta atgtcgttgc ggtgcatcac
     3301 gccttactga ccagtggtct gccggtaagc gaagtggata ttgtttgcac acttcctctg
     3361 acagagtatt acgacaggaa taaccaaccc aatacggaaa atattgagcg taagaaagca
     3421 aacttccgga aaaaaattat attaaatggt ggggatacat tcacaataaa agatgtaaaa
     3481 gtcatgcctg aatctatacc agcaggttat gaagctctac aagaactgga tgagttagat
     3541 tctttattaa ttatagacct cgggggcacc acattagata tttctcaggt aatggggaaa
     3601 ttatcaggga tcagtaaaat atacggagac tcatctcttg gtgtatctct ggtcacatct
     3661 gcagtaaaag atgcactttc tcttgcgaga acaaaaggaa gtagctatct tgctgacgat
     3721 ataattattc acagaaaaga caacaactat ctgaagcaac gaattaatga tgagaacaaa
     3781 atatcaatag tcaccgaagc aatgaatgaa gcacttcgta aacttgagca acgtgtatta
     3841 aatacgctca atgaattttc tggttatacg catgttatgg ttataggcgg tggcgcagaa
     3901 ttaatatgcg atgcagtaaa aaaacacaca cagattcgtg atgaacgttt tttcaaaacc
     3961 aataactctc aatatgattt agttaacggt atgtatctca taggtaatta ataatggaca
     4021 agcgcagaac cattgccttc aaactaaatc cagatgtaaa tcaaacagat aaaattgttt
     4081 gtgatacact ggacagtatc ccgcaagggg aacgcagccg ccttaaccgg gccgcactga
     4141 cggcaggtct ggccttatac agacaagatc ccagggcccc tttcctttta tgtgaactgc
     4201 tgacgaaaga aaccacattt tcagatatcg tgaatatatt gagatcgcta tttccaaaag
     4261 agatggccga ttttaattct tcaatagtca ctcaatcctc ttcacaacaa gagcaaaaaa
     4321 gtgatgaaga gaccaaaaaa aatgcgatga agctaataaa ttaattcaat tattattgag
     4381 ttccctttat ccactatcag gctggataaa gggaacttaa tcaagttatt tcattagcaa
     4441 tgattacata aaataaatac agtgtcgtta ttatgaaatt atttcacggc acttataaaa
     4501 aaacagtacc aataatcaaa actggtgagt tcgcaatgtc tggcaataac gtttttgatg
     4561 ggttgttttc cagcccggag cgcgatattg ccaccagtca cagaaacacc gagaacacca
     4621 gatttcagcg gtcgctgaat ttgaacgaga tcttttactt gaacgaactt attcagggat
     4681 agcaagagca cgggatgcag gaaaacgttt tggtcgccca ccggtattaa atgaagaaca
     4741 aaaacagaag gtactcgaac gcattaagtc tggggcaagt ataagtgcta ttgccaggga
     4801 attccgaacc acacggcaaa caattctgag agttagagcg aaacagcaaa ctgttactgt
     4861 atagaaaata gtcttagcgt gggattccgt tttccaagcg gaccccaagg aagactacat
     4921 cgcgttcatg ccgaaaccga atgtaagaac ggcattgcat aatcttgcag tggcgatcga
     4981 acattacaat gaaaaccatc cgcacagtgc gttgggttat cgctctccgc gagaatatcg
     5041 acgtcagtgg gtaacgttaa cttaagatac aacccctgtc tggaaataag ggggcaagaa
     5101 cacattgaca ctgtaaatat ggcattgtat aaaactccgc cccaataatg agtttcattt
     5161 gcattactcc catttgctgg tcatccactc gcctgtttac aaggatagcg tttaatgaag
     5221 aaggtccttt gcgcgttagg cttgatgttc accgccgtca gttccgcgct ggcaacaacc
     5281 taccccctga cgattgaaaa ctgcggctat caggagacct tcacccggcc gccggagcgc
     5341 gtggtcgcgc tggggcaaaa tacggtcgaa attctgctgc tgctcgggct gcaaaagcag
     5401 gtggtcgcca gcgccttctg gccgaccagc gttttaccgc agctggctga gcaaaacgcg
     5461 aagattaaaa ccttgacggt ggaaattccg agcctggaat cggtgctggc gcaaaatcct
     5521 gatttcgttc ccgcccagtt gccgctgctg ctggggccgg aaagcaaagt cgccaggcgt
     5581 gaagatctcg ccaccgtcgg cgtgaacagc tacgtctcac cggggatgtg cgccaccaaa
     5641 aaggccaccg gcgatatgta cggcagccgg caaaagctgt gggatatgac ctggctgtat
     5701 caggaaatta gcgatttttc ccgcattttc aacgtcgaag atcgtggtca ggcgctgatt
     5761 gccgatttca aaaaacgtga agccgacctg cgccaggagt ttggtaaaag caaaaaggat
     5821 ctctccttcg tcttctggtt ctccagcgcc tcaccttcgg ccgatgccta tgtcggcggc
     5881 aaaaacagcg cctccggatt tatcgccagc gtgctgggcg gacataatgc gattacctcc
     5941 gaaaccgagt ggcccacggt gagctgggag agcattattg ccgccaaccc ggatgtgatc
     6001 gtggtcgcca gcctggatcg taaccgctgg gcgctggata aggccgagga aaagatcaaa
     6061 ttcctcaaaa gcgatcccgc cgtcagccag ctggaggcgg tgaaaaaagg ccatatcgtg
     6121 gtcatggacg gtcaggcgat gaatccgacc attcgcacgc tttacggcgc ggagcaggtg
     6181 ggcgagcagc tgagaaaaat ggggctgaac tgatgagcgt cgccgcgatt gagacgcgcc
     6241 gcagcatact gctcaccggc tggtgtgtac tggcagcgat cgtgctggcg ctggtgatcg
     6301 ctgtgggcgt cagcgttggc gagctggcca tcccgctgca gaatgtcttt tatgctatca
     6361 gcaacagaac cggcttaacc gttgagccgc tcaaccgtat ctatgagagc gttatctggg
     6421 atttccgcct cagccgcgcg ctggtcgccg cctgctgcgg cgccggcctc gctatctgcg
     6481 gtgttgtgct gcaaagcttg ctgaagaatg ccctcgccga accctacgtc ctcggcgtct
     6541 ccgccggggc ctccaccggc gcggtgtcga tcgtggtgct gggccttggc gctggagcaa
     6601 tttccctctc ggcaggcgcc tttgccgggg ccttcgccgc cttcgccttt gtcgccttgc
     6661 tgactaacgg cgcgcgcggc ggcaatgagc gtacgattct tgcgggcgtc gccgcctcgc
     6721 agttatttaa cgccatcacg gcctatacca tcagcacctc cgccagcgcg cagcaggcgc
     6781 gcgatgtgat gttctggctg ctgggcagct tcagcggcgt ccgctggccc gaattccagt
     6841 tggtcatcgt ggtggtgctc gcgggtctgg cggtctgcct gtggtatgcc cgggcgctgg
     6901 acgcgttcac cttcggcgac gatgccgccg cctcgctggg gattgctgtg ccgcgcgtgc
     6961 gcctgatcct cttcaccact gcggcgctga tcaccgccac catcgtcagc atggccggct
     7021 caatcggctt tgttggcctg gtggtgccgc acgtgatgcg tttcttcttt ggcccgctgc
     7081 accgaacgtt gctgatcgcc agcgcgctgg caggggcgat attgatggta ctggcggaca
     7141 ttgcgtcgcg cctgctgatt gccccgcaaa gtctgcccgt tggggtagta accgccctgg
     7201 ttggggtgcc tttctttgcc gtgattatct accgctcaag aaataagtga tgagtatttg
     7261 cgctgaaaat atcacctgga aggcaggcaa aaaggtcatc gtcaataatg tctcgctgcg
     7321 ggtgccgcgc ggcgaaacgg tcggactgct ggggcccaac ggctgcggca aatcctcgct
     7381 gctgcgcgtt ctggcgggcc tgcgccgccc ggatgcaggt cgcgtcaccc tcgacggcca
     7441 ggatatcgcc cggatggcga aaaagcagct cgcccgccgc gtggcttgcg tcgagcaaca
     7501 cggcatgacc gaggccaata tgcgggtgcg cgacgtcgtg cgcctgggac gcattcccca
     7561 ccactctccg ttctcaaact ggagcgctca ggatgacgaa gcgattgccg ccgcgctgca
     7621 gcgggtagcg atgctggaga aaagcgaaca gggatggtta agcctctccg gcggcgagcg
     7681 gcagcgggtg catatcgccc gcgcgctggc gcagagcccg agcgaaatcc tgctggatga
     7741 gccaaccaac catctggata tacaccatca gatgcagtta atgcagttga tcagcgagct
     7801 gccggtaacc agcattgtgg ccattcacga tcttaaccat gccgcgatgt tctgcgattc
     7861 gctgatcgtg atgcagcagg ggcagatcct cgccagcggg acgccggagg agattttgtc
     7921 cgaagcgctg ctgtgggacg ttttccgggt gaaaaccaaa atcgagatct ccccttacca
     7981 cggcaaaaag cacattcatt tcatcgttta ggttgggcgg atatgcctct tttctccctg
     8041 cgccctgcgg caaccctctg gcctccggtg ttactgggaa gccagttcgt ttttaatatt
     8101 ggcttttacg cggtcgttcc tttcctcgcc ctcttcctgc gcgacgacat gctgctctca
     8161 ggcggactta tcggcctgat cctcgggctg cgtaccttct cccaacaggg gatgtttatc
     8221 ctcggcggga cgctggccga ccgctatggc gctaaggcga tcattctcgc cggctgcgtc
     8281 gttcgcgtcg ccggttttct cctgctggcg tgcggggcgt cgctgtggcc cattatcctt
     8341 ggcgcctgcc tgaccggcgt cggcggcgcc ctgttctccc cgtcgataga ggccctgctg
     8401 gcgcgggccg gtacccacag ccaggccaac gggaaacgca gccgcgccga gtggtttgcg
     8461 ctgtttgcgg tatgcggtga actgggagcg gtgatcggcc cggtggccgg aggcgtgctg
     8521 agcgggatcg gctttcggca tatcgccctc gccggcgcgg gaatatttct tctggcgctg
     8581 gcggtgctct ttttcggtct gcccgccgac gggcacacga caacaacgcg cagacgagtc
     8641 ccatggtgga cgcctttgcg ccagccgcgc tttgtcgcgt ttattctcgc ctacagctca
     8701 tggctgttga gctacaacca actgtacctc gccctgccgg tggagatcca acgttccggc
     8761 ggccgcgagc aggatctggc gccgctcttt atgctggcct cgctgttgat tatcaccctc
     8821 cagctccctc tcgcccgctt cgcccggcga atgggcgccg tgcgcatcct gccggtgggc
     8881 tttctgctgc tgtcggcttc ctttgccagc gtggcgttat tcgccgccgc cccacccgcc
     8941 gaaagttggc tgcggttaat gccggcggct ggttttgtca cgctgctcac cctcggccag
     9001 atgctgttag tgcccgcagc caaggatctg atcccgctgt tcgccgagga gtcgacgctg
     9061 ggtgcccact acggagcgct cgccactgca ggagggtgcg cggtgttagc cgggaattta
     9121 ctgctcggtc atttacttga ccaggcgctc attccgtcgc cgcaggcggt ttatccctgg
     9181 cttctgctgg cgctgtttcc gctgtgcagc gccgtcgccc tgcgggcgat ctgtcgcccg
     9241 ctggcggcaa catgacgcag agcgggtaac agcccgctct ggaagtgaac agctggtatc
     9301 catattttca gctaatcctt tagcaaagat cctcagccct gtttaaaccc cggcttgccg
     9361 tttttggctc gccattcctt cacttctttt acgatacctt caggactgtc ctcacggtca
     9421 tcccttggat aataaataag atcagaacca tcagggtgtt cagctaatcg acgaaattca
     9481 tccactaatt tatcgtcatc agcctcagtt tcaccttcag cccggcagat ttttctgacg
     9541 aactccagtt cagttgatat tctctcgcca aagcaggtgg ggctggatat aggtttggag
     9601 tatttcaaat acctggaaga tcaattttgt gcggcagctt ttaattttct acgcaatgct
     9661 gatatgactc aaaacgatgt gaaagataca gagtcagctt atcgtggatg gctggagatt
     9721 gacgaagaag atcttgagta aagctcgata attcttctga cgttataact aacggatgtc
     9781 gcgtaagcga gccttcaggg ccgcttacga gcgagtatga cagacgtata gggtttcttc
     9841 atgttctcag tttttgaatt gtcaattcta ctagttcaac tcaatactct tagcatggga
     9901 aaatttcatt tctccaggcg gctcccaaac cgtgattcct ggtaagcccg catcaagccg
     9961 ggcactcaaa gaatgaatcg gggattctga cgctggccta caaccgccag gatattgaca
    10021 cgacgggcct tatcattcac ttcataaagt acatgctgcc caagtaagct aatacggcga
    10081 accccttcgc catagcgttc atctgtttca tacagcctgg ggtaatctgc cagacgatcg
    10141 gttgcagtga tcagacgctc tgtcacgttc cttgcatccc aaagagatgc attctcggcc
    10201 agatattcaa acaacgccgt tatatcctca atcgcacctg gtcgccattc tatttcataa
    10261 ctcattgcgc agttcctgat aacgtccctg tagttgctgg cgcagttgca cggaactcat
    10321 tcccggccgc ttatcggcgc gagcagcggc aactttcgct cggatctgtt cgtcgtattc
    10381 cgcccgtgcc agttcttcat tagcgaggcg ttgcaatagt gcagacttgt ctgtactgcc
    10441 ttctggaaaa atacgaggca ggctttcggc cagctcgcgc aggaaattgg cgttttcgta
    10501 atgtgtggtg ctattcataa ttgactcccc ctttcattca ataaagggca attgcagttt
    10561 ttttgatgcc gcacgcatgg gtataacgag tcagagtatc gatattcgcc ccggtgatat
    10621 tgtttcacag gcaggcgata accggatgca taatgctcat ttgcttggca atctgcgtgt
    10681 tgtcaggttt tatgcagagt atataacctt tcagacacag ccaccaacat atgcttatgc
    10741 cgcgccagcg gcatgtaagg cggcacgctg tggttacatg tgataccgga gtaaaaccgc
    10801 cgaagcccgg cgtaagccgg tacggattga tagatttcac cttacccatc ccttgccctg
    10861 ccagaccata cccgttttca gccatgagag agcttctgtg cgcggtcgga gtggtcccga
    10921 cgagggttta cccgaagtcg gggcgtatct ccgcgttagc gggccgtcag ggccgcttac
    10981 gagcgtgtat tcaatacgtc cagccagaag acttacagcg atgatgatgc ttagttacaa
    11041 cattcataat taaaagcgac tctgttccgg cccgaagggc cggggcgggg ccgcttttca
    11101 gttatgaggg aggggctttg tggtttcagt tctgcgctgg acctaggttt tcccggtggt
    11161 tgtatttgag tgttgtaact aaagtggctc cggccggggc ccgccgcttg cggtgggagg
    11221 tgcatatctg tctgtccaca ggacaggcag tgaacaggtt ttctttttaa atgaatgtaa
    11281 ttaagtagtt taaaggagat ataaacaggt gtttaaaaga tatattgcac cctgtaaggc
    11341 tgacagctgg cgctttatga catgaacggt tgtaacctta tggggaagtc ccttgcagtt
    11401 taatgtggat aagcaaaatt ccccgtctgt gaggcgtgtt ttgtatcaaa aacaggggga
    11461 ccggatgcac cagaaggtgg atgatgaggt tgttttttgt atgtagcgct gattttttgt
    11521 gcactggcgg gcttcaggcg tgcgaatgcc tccggcgcgt gccggattat tctgaggagg
    11581 tcactttcag ggggaagctg tgaccagccg gctgtaatta cggttacgtg acagaatcat
    11641 gcgctccttc acacgacgct ccacttcgcg ttttaccgcc tcacgattag ctgtgaagcg
    11701 cccttccgag atttcacgcg tcagctgccg tttcaccagg ctgacgatat cctgacgttc
    11761 cctgttcgca tcacgacgcg cacgggcacg ttttattcca cgggacttaa gctctgtctg
    11821 gtaactgcgg aaacgctcac gcacaaaacg ccaggctttc gctatcagtt catccatacc
    11881 cagtgcatcc agtccctgct tcttgcgctg tctgttttcc cattccacac gactgcggcg
    11941 cgcggacgcc accgcctcct ctgatacatc gagggcggca aacagcgccg gtgtgaacgt
    12001 gatgtcggtc ggaatgttgc agccgataag cgggtcatat tccgtctggt aggtaatcag
    12061 ccccagctct gccaggaacg tcagggcacg cgtggcacgg gtgatggaaa gtgttccggc
    12121 atcagactcc gtcgccagtc cgcactcaat ggccagcgtg gtgatggagc actgaacgcg
    12181 gttggccagc gggtcatagt ggaaacacag cccctgcagc agcgcatcaa tggcccggcg
    12241 acgtagtacc ggtggcatgc gccgacgcag accaagcgaa cgggcatgtg ccacatgaat
    12301 ggcgaaatca aaacgggagg tgaagcccac cgccttttcc atcagtttcc cgcagaacgg
    12361 aagcgtccgg gcaccttcac ggggtgtgaa caccggattc gggttcttta cctggcggta
    12421 ataagtttgt tgaagatcag tcacaccatc ctgcacttac aatgcgcaga aggagtgagc
    12481 acagaaagaa gtcttgaact tttccgggca tataactata ctccccgcat agctgaattg
    12541 ttggctatac ggtttttaga tggccccggt aatcttctca gtcgccaaac ttgtcgaaga
    12601 ttatcggggt tttcactttt ctggctcctg taaatccaca tcagaaccag ttccctgcca
    12661 ccttacggcg tgaccagcca caaaattcct taaacgatca gcaatctatc acgcacacca
    12721 gaaacaggca agaatgtgat gtcgctacat tttagttagc taaaagcact cttctgcttt
    12781 atcagttcgg caatcatctc agcctgggta acgccctcag cttcacacat cacctgcaat
    12841 tgcgctttta acgcagcagg aatgaacact ctgagttctt tatgtgtagc tcgttttctt
    12901 gccagggatg cctgctgacg ttctgcaagc gtcagaggat taccttttct gtatgctcgc
    12961 tttgtacctg atgaggaagt cactgcattt tctgtctgcg acatctcgcc tcctcaatac
    13021 tcaaacaggg atcgtttcgc agaggatact acagtttttt gaaatcagcg acttgagaat
    13081 tgtgacgaag atctgtgttg gcgcacaaat caacggggat tactgtcgtt taatgtgatt
    13141 taaactgtga aatagtatgg ttttcagtta tggaaacgcc gggagcgggg aaaacttgct
    13201 ttttcccgtt tccggggttg gacaactgag caacgcgaag gcgtcagcta cgatgttccg
    13261 gggactgctg atcagcccct gttgtattca gccattctcc ggtcatctgc agcagttttc
    13321 tggcatcgtg gcttctgagc aacggaatac cagcctgcct cagaacctct tccagaagaa
    13381 tatcccggcg tctgcgttcc ggtcgtaaat ggctggcatc atccagctct actgctgcaa
    13441 caatactgaa cgaacgccgc tcaacgataa ccacatcaac atgccactga gacaccatcc
    13501 tgaataactg ccaccactgg cgtgatcgtg gccggatatt cccgttcagc tggacgatat
    13561 ccgcgacccg tacctgcgga cacagatacc accgcttcat atccacaatt ctgaacagtc
    13621 ctttcataaa actgacttcc ctttctgtca gcagcgtctt ctgacgttgc cagtattcac
    13681 cgccggacag tattcgctct gcatcaccgg tccgtatacc tgccgcatgc aactgcatca
    13741 gcaaaggaga atgctgattt acacttctga ggataatccg gataagtaac gctgcaataa
    13801 tcagaacaat gagaataaaa ataatcagct tcattgttta tctgctcctg gtaattaatt
    13861 attctgaatt attaccttac atatttccat atctcttttg gtatcctgtc atataacttt
    13921 ccggtgatta attccgctaa ccggtggtct gctgctgaat taaatgattc acgttcactg
    13981 tttaataaac tgtctctgtt tttatgaata attttttcca gcgtattgac tgatgaacat
    14041 cgccgtaact gatacagcca ttcctgtttt gttttcgcca tttttattcc cgctgagaac
    14101 agatgaatga taagattgcc atcgctttat ttacggtgtc tccatatcca ggggggaacc
    14161 ggatcagcat ctgaccagag cccacgtttt tgttgtctgg cttcgttctg aaggacaggc
    14221 agcacgggat ctgtgttgta ctgctcatat acccaggcag ccccggcccg gaccataaac
    14281 tgattaacat tcattccgtc tggtgcataa acctgcccga gaatacgacc ataacgatct
    14341 cgctgaaaat atgtgacggt aaccgttttt cctgcaacca gtgatttcat catgtccgtt
    14401 gaccaccggc cataatcctg tttcttttcc ggtgcatcaa tatttaccag cctgatccgc
    14461 accgccttcc gtgagtccat gacctcgatg gtatcaccgt ccagtacacg aacaacccgt
    14521 ccatgaatat ctgcacacaa taccggccat gaaaagataa ataataccag tggaatatat
    14581 ttcctcatgt tattctccat atcagataaa tggcacctgt atttcaggta atcctgaata
    14641 tcgcagcaca cactgaataa caccttattt tgtgaaatta atcacaaaat ataaaatcac
    14701 gacttaatac ggaaaaaact ctgtgctact cagaactgtc cggtcatact gcccgaccgg
    14761 atattaaaaa aagcaaatct catgcattat tcagccttct gaatccctgg agtaatgaag
    14821 gctgcttttt cgttacaccc ttccccggca gcgccggtgg cattgttcag ccctttttcg
    14881 ggcaaactgc tgccagtctg tccggggtgc aacaccggca gcccgtcgcc atgcttctgc
    14941 cgcttccatc cagcgtccac tgacctccag aataacggcc cggcttccgg cacgctgcca
    15001 gcgccagacc tgaccgttcc gtgaactgtt tctgaatcca ttcatcttta tcactcctct
    15061 ttaacccgtc agtattatgg tgttaaataa tctactgaat tggcttcctt tcgggtacgg
    15121 aagttatttc cttttaagcc ttatttccct gcctcctctc tgatggaggc tttttttatt
    15181 tctcatcaag cacggcctga agctctgcct ttatcctgtt ctggcggcgg attttatcca
    15241 gacgctctgc cgcatacact tcctcctcct ga
//
LOCUS       DAKISA010000100        15148 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.100, whole genome shotgun sequence.
ACCESSION   DAKISA010000100 DAKISA010000000
VERSION     DAKISA010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15148
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.100"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            455..1381
                     /locus_tag="OVW45_003318"
     CDS             455..1381
                     /locus_tag="OVW45_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCV5527688.1"
                     /translation="MENWVIQELKSLDIGDSRLDKRVKHILNSLSRSPEESIPVSCRT
                     WSETKAAYRCFSNDKVSANKIMAPHKENITERAQQFKRVLVLQDTTELNYSGQKEKQG
                     VGHKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKNTRQEICNDLLHKK
                     HVSEKETWRWVEGYNKATELARQCPDTHVLSISDREGDFYDLFERAEQTPGIKADWLV
                     RMKFNNRATLNINGKRDHRLLHERIMEITPQQLVEFTIPDGRGQQARTLPDMLSSDAG
                     PLSYPLIYSSKVMIISLFDSTVMPDKYRHNGK"
     gene            complement(1331..2524)
                     /locus_tag="OVW45_003319"
     CDS             complement(1331..2524)
                     /locus_tag="OVW45_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCV5527689.1"
                     /translation="MSPGIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFVTDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2660..4384
                     /gene="iucA"
                     /locus_tag="OVW45_003320"
     CDS             2660..4384
                     /gene="iucA"
                     /locus_tag="OVW45_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCV5527690.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSSTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            4385..5332
                     /gene="iucB"
                     /locus_tag="OVW45_003321"
     CDS             4385..5332
                     /gene="iucB"
                     /locus_tag="OVW45_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCV5527691.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            5332..7074
                     /gene="iucC"
                     /locus_tag="OVW45_003322"
     CDS             5332..7074
                     /gene="iucC"
                     /locus_tag="OVW45_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCV5527692.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFVEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDATSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            7071..8408
                     /gene="iucD"
                     /locus_tag="OVW45_003323"
     CDS             7071..8408
                     /gene="iucD"
                     /locus_tag="OVW45_003323"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCV5527693.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            8414..10609
                     /gene="iutA"
                     /locus_tag="OVW45_003324"
     CDS             8414..10609
                     /gene="iutA"
                     /locus_tag="OVW45_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCV5527694.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLSLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYCPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(11160..11303)
                     /locus_tag="OVW45_003325"
     CDS             complement(11160..11303)
                     /locus_tag="OVW45_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5527695.1"
                     /translation="MEHRSVIHGELAIDHDAILQCAEEVLHHCVIQTVSFVWHILCYS
                     MGL"
     gene            11542..15148
                     /gene="sat"
                     /locus_tag="OVW45_003326"
                     /pseudo
     CDS             11542..15148
                     /gene="sat"
                     /locus_tag="OVW45_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
BASE COUNT         4128 a         3597 c         3720 g         3703 t
ORIGIN      
        1 cgaaggggcc gacaaacacg ttgtccctga gttcacattc ataaatgttg accggtttga
       61 caatacggac attgatccca taaatcacat cccggacagc acattccaga aatttcatac
      121 tcacacgtcc tttaaataca gatgacattt gccggaatta taccgcccag attcacttgc
      181 catgtttcag aatagcttat aagccgcact tttaccgata ctcagcaaca aatcaggtct
      241 gtatccactt cagccggaaa ccggatctgc gcggcgggga acgggcaaga aatatgataa
      301 ttaagcaaca cacataaaat catattgcat aatcgcatct gaatcgaaca cttattcgca
      361 tcactatcga tcatcacttc gcatctcata cccttaccca caagttgaaa taatagtatt
      421 atattgaaaa gacagagatt atttcagagg tgttatggaa aactgggtta ttcaggagct
      481 aaaatcactg gatatagggg actcaagact tgataaaaga gtaaaacata tcctcaatag
      541 cctcagtcgt tcaccagaag aaagtattcc tgtttcctgc cgtacctgga gtgaaacaaa
      601 ggcagcttat cgttgttttt caaacgataa agtctctgct aataaaatta tggctcctca
      661 caaagaaaat atcactgaaa gagctcagca gtttaaaaga gttttagttt tacaggatac
      721 aacagaactt aattattctg gtcagaaaga aaaacaagga gttggtcaca aaaaacataa
      781 agatgaaaga aacttatttc ttcatcctca actcgtcatc agtgagtcag gtgtttgcct
      841 cggtgtttat gatgactatc agtggttcag ggatgaatta aaaaccagta aaaatacccg
      901 acaagaaatc tgcaatgatt tacttcataa aaaacatgtc agtgaaaaag aaacctggcg
      961 ttgggttgaa ggatataata aagcaacaga actggctcgc caatgccctg atacccatgt
     1021 attgtcaatt tctgatcgtg aaggcgattt ttatgacctg tttgaacgcg cagagcagac
     1081 tcccggaata aaagctgact ggcttgtcag aatgaaattt aataacagag caacgctgaa
     1141 tattaatggt aaacgggatc ataggttgtt gcacgaaaga atcatggaga ttacaccgca
     1201 gcaactggtt gaattcacta tacctgatgg gcgggggcag caagccagaa cactgcctga
     1261 catgctttca tcagacgcag gtcctctctc atatcctctg atttactcca gtaaggtgat
     1321 gatcatatct ttatttgata gtacggtaat gccagataaa tacagacaca atggtaaata
     1381 atatagttgc aaataatgct gccataatca gcgcgattgc gacacctgtt ttcgatacca
     1441 gcggcaccag caaagcgcac agtccatagc caagtgtgtg actggtagcg agccagcccg
     1501 cgcctgcgcc atcagccagc ttatcattca gtagaagttg ataagcgggt gttgccagag
     1561 ctgctccaaa tgacagcact gcacagcccg ggtaaaacaa ccatagctgt tcggagagca
     1621 tgatagccag accaccactc atcaacactc cggcactcag gagcagggcc accggagtca
     1681 ggcgctgcgg acggagtacc ccgaactgcg cgataagcgc agctactgcg gacagaccca
     1741 acagccaggc cacctgttgg ctaatggcgg tggtatccgt gacgaactgg cgagtaaggg
     1801 caggcgaaag tccaagctgc atcatgctca ccgccgcagc cagtaatatt gcgcaaagca
     1861 gataaggcag acaatcccgc ttcagactga cgctcttgca ctccggtgtg ggctgtggtg
     1921 gtgtgccggg caaccgcagc agcatcagca gcgccagcac tggtgctgcc atcagtagcc
     1981 ccagtggcgc cagaggatga atggccagca ttgctgccgc gcacagcggc ccgaatagcc
     2041 tgccgcaact caggccggag ctgatggttg ccagagcggc catgcgattc ccttctccag
     2101 ctctctgcaa tgcccagacc tgacaggctg gcaccattgc cgacacggtc agcccgtagg
     2161 cgatccgggc gatgattaat attcccagtc ccaccgctgt tgttatggcg ctggtagcca
     2221 tcagcacgct tcccagccct agcagggtaa agcttatgcc atatcctccc agagcccaca
     2281 acaccacagg tttactgcca gtacgggaaa tttgctttcc ccaccacggg gacgatggca
     2341 gaaacagcat tgagccaagc atcagcaggg cagcccatac agacagactc agatttgtct
     2401 gtatgaccag aacagggagc acaaccagca gaccgttctg tccgataccg agaagcccgg
     2461 cactgaacgc aagtggccag caggacagtg ttttttgggg cgtatcttcg atccctggtg
     2521 acattataat gtttcaactc catgtattaa ttgtgtttat ttgtaaaatt aatttatctg
     2581 acaataacat ttattgttga taatgagaat cattatcgac ataattgtta ttattttact
     2641 gtgtgggagc tgtttgacta tgaccctgcc ctctgaaaaa ccagccacag atgtggctgc
     2701 gcagtgcttc ctgaatgcac tgattcgtga aaccacagac tggaaactga cagaataccc
     2761 gccagacgaa ttgctcatcc cgctggatga gcagaaatcg ctccatttca gagtggctta
     2821 tttctcctca acccagcatc accgctttgc atttcctgca cgtctggtca cggcatcagg
     2881 cagttatcct gtcgacttta ccactctctc ccggcttatt attgataagc tacgccatca
     2941 actgtttctg cccgttcccc tctgcgaaac tttccaccag cgcgtgctgg aaagccatgt
     3001 ccatacgcaa caggcaattg atgcccgtca tgactgggcc gctctgcgtg aaaaagcgtt
     3061 gaattttggc gaggctgagc aggcgctgct gacaggacac gctttccacc ctgcgcctaa
     3121 gtctcatgaa ccgtttaacc gacgggaggc tgaacgctac ctgcctgata tggcacccca
     3181 cttcccactg cggtggtttt cggtggataa aacgcaaatc gccggtgaaa gtttgcatct
     3241 caaccttcaa cagcggctga cgcgatttgc cgcagagaat gcgcctcagt tactcaacga
     3301 attaagtgac aaccaatggc tgttcccgtt gcacccgtgg cagggggaat atcttttgca
     3361 gcaggggtgg tgccaggcac ttgttgctaa agggctgatt aaagacttag gtgaggccgg
     3421 cacgtcgtgg ctgccgacca cctcttcccg ttccctctac tgtgccacca gccgcgatat
     3481 gatcaagttc tccctgagcg tacggctgac caactccatc cgtactctgt ccgtgaaaga
     3541 agtgaagcgt gggatgcgcc tggcacgcct ggctcaaacc gacggctggc agatgctaca
     3601 ggtccgcttc ccgactttcc gggtaatgca ggaggatggc tgggccgggc tgctcgatct
     3661 taacggcaac atcatgcagg aaagtctgtt tgccctgcgt gaaaatctgc tggtggatca
     3721 gccgaaaagc cagactaacg tactggtctc cctgactcag gccgcacctg atggcggtga
     3781 ttcgctgctg gtttcggcgg taaaacgcct gagcgatcgc ctcggtatca ctgtgcaaca
     3841 ggccgcccat gcatgggtcg atgcatactg tcagcaagtg ctaaagccgc tgtttacggc
     3901 tgaagcggat tacggcctgg tgctgctggc gcatcagcaa aatattcttg tccagatgct
     3961 tggggatctg ccggtcggat ttatttaccg tgactgtcag ggcagcgctt ttatgcctca
     4021 cgcgacagac tggctcgatt ctattggcga ggcgcaggcg gaaaatattt tcacccatga
     4081 gcagttgctg cgctatttcc cttattacct gctggttaac tccacttttg ctgtgaccgc
     4141 tgcgctgggg gctgccgggc tggacagcga atcgaatctg atggctcgtg tacgagcatc
     4201 gctggctgaa gtgcgtgatc aggtgactca taaaacctgt ctcaactacg tgctggaaag
     4261 tccgtactgg aacgtaaaag gtaacttttt ctgttatctg aacgatcata acgagaacac
     4321 cattgttgac ccttcggtga tctatttcga tttcgctaac ccgctgcagg ctcaggaggt
     4381 ctgaatgtct gaggcaaaca ttattcacag cagatatgga ctgcgctgtg aaaaactcga
     4441 caagcccctg aatcttggct ggggactgga caatagcgcg gtgttgcact gtcccgggga
     4501 gctgccgaca gggtggctgt gcgatgcgct ggatcagata tttatcgccg caccacaact
     4561 gtcagcagtg gctctcccct gggccgaatg gcgtgaggag ccacaggcgc tgacgctttt
     4621 tgggcaggta aaaagcgaca ttatccatcg tactgccttc tggcagttac cgttatggtt
     4681 gagttcaccg gcaaaccggg cctccggtga aatggttttt gatgcagagc gtgagattta
     4741 tttcccgcag cgcccacccc gtccacaggg tgaggtttac cgccgttacg atccacgtat
     4801 tcgcaggatg ctgagtttcc gcattgccga tcccgtttct gatgcagaac gtttcacccg
     4861 ctggatgaat gatccgcgcg ttgagtattt ctgggagcaa agtggctcac tggaggtaca
     4921 gaccgcctat ctggagcgcc agctcaccgg taaacatgca ttcccgctga ttgggtgctt
     4981 cgacgatcgg ccattcagct atttcgaaat ctactgggcg gcggaagacc gcattggtcg
     5041 tcactattcg tggcagccct ttgaccgtgg cctgcatctg ctggttggtg aacagcaatg
     5101 gcgcggggcc cactatgtgc aaagctggct gcgcgggctg acacattacc tgctgctgga
     5161 tgagccccgt acgcagcgca cagtactgga gccacgcacc gataaccagc gcctgttccg
     5221 ccatcttgag cctgcgggat accggacaat taaagagttc gacttcccac ataagcgctc
     5281 gcgcatggtg atggcggatc gtcatcactt cttcacggag gtcggtctgt aatgaatcac
     5341 aaggactggg attttgttaa ccgtcggctg gtggcaaaaa tgttgtctga gatggagtat
     5401 gagcaggttt tccacgcgga atctcagggc gatgaccact actgcattaa cctgccggga
     5461 gcacaatggc gcttcatcgc tgaacgtggt atctggggct ggctgtggat tgatgcccaa
     5521 acgttgcgct gcacggacga accagtactg gcgcagacgc tgctgatgca gctaaagccg
     5581 gtgctgtcaa tgagtgatgc cactgttgca gagcatatgc aggatttgta tgccacgcta
     5641 ctgggcgacc tgcaactgct gaaggcccgt cgcggactga gtgccagtga cctgattaat
     5701 cttgatgccg atcgtctgca atgcctgctg agcggtcatc ctaaattcgt ttttaataaa
     5761 ggtcgccgtg gctggggcaa agaggcgctg gaacgatatg cgccagagta taccaacacc
     5821 ttcagactgc actggctggc ggtaaaacgt gaacatatga tctggcgctg cgataacgat
     5881 ctggatattc agcagttgtt gactgccgca atggatccgc aggagtttac acgcttcagt
     5941 caggtctggc aggaaaacgg gctggatcac aactggctac cgctgccggt acatccgtgg
     6001 cagtggcagc aaaaaatcgc cacagacttc atcgctgatt ttgtcgaagg caggatggtg
     6061 tctctcggcg agtttggcga ccagtggctc gcccagcagt cgctgcgtac cctgaccaac
     6121 gccagccgcc ggggagggct ggatatcaag ctgccgctga ccatctacaa cacctcatgc
     6181 taccggggaa tccctggcag atatatcgct gccggaccac tggcttcacg ctggctgcaa
     6241 caggtttttg cgaccgacgc caccctggtg caaagcggcg cagtgatcct tggtgaaccg
     6301 gctgcaggct atgtgtccca tgaaggctat gccgcgcttg cccgggctcc ctatcgctac
     6361 caggaaatgc ttggtgttat ctggcgggag aatccgtgcc gctggctgaa accggatgaa
     6421 agtcctgttc tgatggcaac actgatggag tgcgacgaaa acaatcagcc gctggcaggc
     6481 gcatatatcg accgctccgg actggacgct gaaacctggc ttacgcaatt gttccgggtg
     6541 gttgtggttc cgctgtatca cctgctttgc cgctacggtg tcgcgcttat tgcacacgga
     6601 caaaatataa cgctcgccat gaaagagggg gtaccacagc gtgttctgct gaaagacttc
     6661 cagggcgata tgcggctggt gaaagaagcg tttcccgaaa tggactcttt gcctcaggag
     6721 gtgcgtgatg ccacatcccg tctgagtgcg gactacttaa tccatgattt gcagacgggt
     6781 cacttcgtga cagtactgcg ttttatttcg ccactgatgg ttcgtcttgg cgtacctgaa
     6841 aggcgatttt atcaactgct ggcagcagtg ttgagtgatt acatgaacaa acatccacaa
     6901 atggcagagc gttttgcgct tttctcactc ttcaggccac aaatcattcg cgtggtgctg
     6961 aacccggtaa aactgacctg gccggacctg gatggcggca gtcgcatgct gccgaattac
     7021 cttgagaatc tgcaaaatcc gctgtggctg gtaactcagg aatatgaatc atgaaaaaaa
     7081 gtgtcgattt tattggtgtc gggacagggc catttaatct cagcattgct gcattgtcac
     7141 atcagatcga agaactgaac tgtctcttct ttgacgaaca tcctcatttt tcctggcatc
     7201 cgggtatgct ggtaccggat tgtcatatgc agactgtctt tctgaaagat ctggtcagtg
     7261 cagttgcacc tacaaatccc tacagctttg ttaactatct ggtgaagcac aaaaagttct
     7321 atcgcttcct tacaagcagg ctacgtacag tatcccgtga agagttttct gactatctcc
     7381 gctgggctgc tgaagatatg aataacctgt atttcagtca taccgttgaa aatattgatt
     7441 tcgacaaaaa aagtcgattg tttctggttc agaccagtcg gggagaatat tttgcccgca
     7501 atatctgcct tggtacagga aaacaacctt atttaccacc ctgtgtgaag catgtgacac
     7561 aatcctgttt ccatgccagt gaaatgaatc ttcgtcggcc ggacctgagt ggaaaacgga
     7621 taaccgtggt tggtggagga cagagtggtg cagacctgtt ccttaatgca ttacgcgggg
     7681 aatggggaga agcggcggaa ataaactggg tctcacggcg taataatttt aacgcactgg
     7741 atgaggctgc ttttgctgat gagtatttta cacctgaata tatttcaggc ttctccggac
     7801 tgaaggaaga tattcgccat cagttactgg atgagcagaa aatgacatcg gatggcatca
     7861 ctgccgattc tttactgacc atttatcgtg agttgtacca ccgttttgaa gttctgagaa
     7921 aaccaagaaa tatccgtctg ctacccagtc gctcggtaac aaccctggaa agtagtggtc
     7981 cgggctggag gttattgatg gaacatcatc tggatcaggg cagagagagc ctggaaagtg
     8041 atgtggtgat tttcgccaca ggttaccgtt ctgcattgcc acaaatactt ccctcactga
     8101 tgcccttgat caccatgcac gataagaaca cctttaaagt gcgtgatgac ttcactctgg
     8161 aatggagtgg cccgaaagaa aataacatct ttgcggtcaa cgccagcatg caaactcatg
     8221 gcatcgccga accccagctc agcctgatgg cctggcgatc tgcacgtatt cttaatcgcg
     8281 tactggggcg tgatttattc gatctcagta tgccgcccgc actgattcag tggcgcagcg
     8341 gcagccggaa aaaaccgcag ccggaggctg cttctttaac tcactataca gcaaatattc
     8401 aggaataacg atgatgcgca aaaagtatat gccccgggct cttggtccgc tgcttcttgt
     8461 cgtgctgtca ccagctgtcg cccagcaaaa cgatgataat gagatcatag tgtctgccag
     8521 ccgcagcaat cgaactgtag cggagatggc gcaaaccacc tgggttatcg aaaatgccga
     8581 actggagcag cagattcagg gcggtaaaga gctgaaagac gcactggctc agttaatccc
     8641 cggccttgat gtcagcagcc agagtcgaac caactacggt atgaacatgc gtggccgccc
     8701 gctggttgtc ctgattgacg gtgtgcgcct caactcttca cgttccgaca gccgacaact
     8761 ggactctgtc gatcctttta atatcgacca tattgaagtg atctccggcg cgacggccct
     8821 gtacggtggc gggagtaccg gagggttgat caacatcgtg accaaaaaag gtcagccgga
     8881 aaccatgatg gagtttgagg ctggcacaaa aagtggcttt aacagcagta aagatcacga
     8941 tgagcgcatt gccggtgctg tctccggcgg aaatgaccat atctccggac gtctttccgt
     9001 ggcatatcag aaatttggcg gctggtttga cggtaacggc gatgccaccc tgcttgataa
     9061 cacccagacc ggcctgcagc actccaatcg gctggacatc atgggaaccg gtacgctgaa
     9121 catcgatgaa tcccggcagc ttcaactgat aacgcagtac tataaaagtc agggagacga
     9181 caattacggg cttaatctcg ggaaaggctt ttccgccatc agcgggagca gcacaccata
     9241 cgtcagtaag gggctgaatt ctgaccgcat tcccggcact gagcggcatt tgatcagcct
     9301 gcagtactct gacagtgatt tcctgggaca ggaactggtc ggtcaggttt actaccgcga
     9361 tgagtcgttg cggtactacc cgttcccgac ggtaaatgcg aataaacagg cgacagcttt
     9421 ctcctcgtca cagcaggata ccgaccagta cggcatgaaa ctgactctga acagccaact
     9481 gatggacggc tggcaaatca cctgggggct ggatgctgag catgagcgct ttacctccaa
     9541 ccagatgttc ttcgatctgg ctcaggcaag tgcttccgga gggctgaaca accataagat
     9601 ttacaccacc gggcgctatc cgtcatatga catcaccaat ctggcggcct tcctgcaatc
     9661 cagctatgac attaatgata tttttaccgt tagcggtggc gtacgctatc agtatactga
     9721 gaacagggta gatgatttca tcgactacac gcagcaacag aagattgctg ccgggaaggc
     9781 gatatctgcc gacgccattc ctggtggttc ggtagattac gataactttc tgttcaatgc
     9841 tggtctgctg atgcacatca ccgaacgtca gcaggcatgg ttcaattttt cccagggggt
     9901 ggcattgccg gatccgggga aatattatgg tcgcggcatc tatggtgcag cagtgaacgg
     9961 ccatctttcc ctgacaaaga gcgtgaacgt cagcgacagt aagctggaag gcgtgaaagt
    10021 cgattcttat gaactgggct ggcgctttac cggtgacaac ctgcggactc aaatcgcggc
    10081 atattactcg ctttccaata agagcgtgga aaggaataaa gatctgacca tcagtgtgaa
    10141 ggacgacagg cgccgtattt acggcgtgga aggtgcggtg gactacctga tcccggatac
    10201 tgactggagt accggtgtga acttcaatgt gctgaaaacc gagtcgaaag tgaacggtca
    10261 atggcaaaaa tatgacgtga aggaatcaag tccatcgaaa gcgacagctt acattaactg
    10321 ggcgccggaa ccgtggagtc tgcgtgtaca gagcaccact tctttcgacg taagcgatgc
    10381 agagggtaac gatattaatg gttacactac cgtcgatttt atcagtagtt ggcagcttcc
    10441 ggtgggaaca ctcagcttca gcgttgagaa cctcttcgac cgtgactata ccactgtctg
    10501 gggacagcgt gcacctctgt actactgccc gggttacggc cctgcttcac tgtacgacta
    10561 caaaggccgg ggccgaacct ttggtctgaa ctactcagtg ctgttctgac ggggatgctt
    10621 ttgcaacaag ggtatgctgt aaacaacatg gtccttggtg gccatgttgt ttacagccag
    10681 tataatatac agccttaaca agatattccg tctccggaat ggactgttca ccactgaata
    10741 acattatttc tgttttatgg atctttgact gaaatttatc gcatatcctc atttcattgt
    10801 ccgtctgaaa actaacctta caagtaagcg tcgtctcagc accgtctggc agatcctgaa
    10861 attcctgaga ggatgatctg cgcccaatat cctgagccgg aactttgctg caatgaaggc
    10921 caacgaaaaa tgggggagta tgttatagag tacttgctac agggtaaaaa gaggtacctg
    10981 tcgctggttc tccgttcagt ttaaccggga gattttctcc tgcgcctgtc ataaagtccg
    11041 gtaatggaga ttgttaatac gatggtgcac gatgcattgt cggtgcccgg cccggaggat
    11101 gccccgtaac tacatacgga tcagggttgg caatatcgaa tggcaggcta taggcaaagt
    11161 taaagcccca tggagtagca caaaatatgc cacacaaagg aaacggtctg aataacgcag
    11221 tgatggagaa cttcttcggc acactgcaaa atcgcatcgt ggtcgatcgc taattcgccg
    11281 tgaatcactg aacgatgttc catgcgaaca gatattcgat catatcgaaa tattaaacat
    11341 tacccctccc cgcattgggc aaaaaaaaac aaaaaacgat caattagtat tgattcacat
    11401 aatctctcat cattaatgag aacattgtca catatttaca atatagaact gttactttta
    11461 gtaagctgtt agtaaccaac tgttactaat aattttaatt cttaatactt tgttcattca
    11521 ataaatgagt tgagagaata tatgaataaa atatactccc ttaaatatag tgctgccact
    11581 ggcggactta ttgctgtttc tgaattagcg aaaagagttt ctggtaaaac aaaccgaaaa
    11641 cttgtagcaa caatgttgtc tctggctgtt gcaggtacag taaatgcagc aaatattgat
    11701 atatcaaatg tatgggcgag agactatctt gatcttgcac aaaataaagg tattttccag
    11761 cccggagcaa cagacgtaac aatcacttta aaaaacggag ataaattctc tttccataat
    11821 ctctcaattc cggatttttc tggtgcagca gcgagtggcg cagctaccgc aataggaggt
    11881 tcttatagtg ttactgttgc acataacaaa aagaaccctc aggccgcaga aactcaggtt
    11941 tacgctcagt cttcttacaa ggttgttgac agaagaaatt ccaatgattt tgagattcag
    12001 aggttaaata aatttgttgt ggaaacagta ggtgccaccc cggcagagac caaccctaca
    12061 acatattctg atgcattaga acgctacggt atagtcactt ctgacggttc aaaaaaaatc
    12121 ataggttttc gtgctggctc tggaggaaca tcatttatta atggtgaatc caaaatctca
    12181 acaaattcag catatagcca tgatctgtta agtgctagtc tatttgaggt cacccaatgg
    12241 gactcatacg gcatgatgat ttataaaaat gataaaacat ttcgtaatct tgaaatattc
    12301 ggagacagcg gctctggagc atacttatat gataacaaac tagaaaaatg ggtattagtc
    12361 ggaacaaccc atggtattgc cagcgttaat ggtgaccaac tgacatggat aacaaaatac
    12421 aatgataaac tggttagtga gttaaaagat acctatagtc ataaaataaa tctgaatggc
    12481 aataatgtaa ccattaaaaa cacagatata acattacacc aaaacaatgc agataccact
    12541 ggtactcaag aaaaaataac taaagacaaa gatattgtgt tcacaaatgg gggagatgtc
    12601 ctgtttaagg ataatttgga ttttggtagc ggtggtatta tctttgacga aggccatgaa
    12661 tataacataa acggtcaggg atttacattt aaaggagcag gaattgatat cggaaaagaa
    12721 agcattgtaa actggaatgc attgtattcc agtgatgatg ttttacacaa aataggcccc
    12781 ggtactctga atgttcaaaa aaaacagggg gcaaatataa agataggtga aggaaatgtt
    12841 cttcttaatg aagaaggaac atttaacaat atataccttg caagcggaaa tggtaaggta
    12901 atactaaata aagataattc ccttggcaat gatcaatatg cggggatatt ttttactaaa
    12961 cgtggtggca cgctagattt aaatggacac aatcagactt ttactagaat tgccgccact
    13021 gacgatggaa caacaataac taactcagat acaacgaaag aagccgttct ggcaatcaat
    13081 aacgaagact cctacatata tcatgggaac ataaatggca atataaaact aacgcacaat
    13141 attaattctc aggataagaa aactaatgca aaattaattc tggatggtag tatcaacaca
    13201 aaaaatgatg ttgaagtcag taatgccagt cttaccatgc aaggccatgc aacagagcat
    13261 gcaatattca gaagctcagc gaatcattgc tccctggtat ttctttgtgg aacggactgg
    13321 gtcaccgttt tgaaagaaac agagagttca tataataaaa agttcaattc tgattacaaa
    13381 agtaataatc agcagacctc atttgatcag cctgactgga aaaccggggt gtttaaattt
    13441 gatacattac acctgaacaa tgctgacttt tcaatatcac gcaatgccaa tgttgaagga
    13501 aatatatcag caaataaatc agctatcaca atcggcgata aaaatgttta cattgataat
    13561 cttgcaggga aaaatattac taataatggt tttgacttca aacaaactat cagtactaat
    13621 ctatccatag gagaaactaa atttacaggt ggcatcactg cacataacag ccaaatagcc
    13681 ataggtgatc aagctgtagt tacacttaat ggtgcaacct ttctggataa tactcctata
    13741 agtatagata aaggagcaaa agttatagca caaaattcca tgttcacaac aaaaggtatt
    13801 gatatctccg gtgaactgac tatgatggga atccctgaac agaatagtaa aactgtaacg
    13861 ccgggtctcc actacgctgc tgatggattc aggctgagtg gtggaaatgc aaatttcatt
    13921 gccagaaata tggcatctgt caccggaaat atttatgctg atgatgcagc aaccattact
    13981 ctgggacagc ctgaaactga aacaccgact atatcgtctg cttatcaggc ataggcagag
    14041 actcttttgt atggctttga taccgcttat cgaggcgcaa taacagcccc caaagctaca
    14101 gttagcatga ataatgcgat ctggcatcta aatagccagt catcaattaa tcgtctagaa
    14161 acaaaagaca gtatggtgcg ttttactggt gataatggga agtttacaac ccttacagtg
    14221 aacaacctta ctatagatga cagtgcattt gtgctgcgtg caaatctggc ccaagcagat
    14281 cagcttgttg tcaataaatc gttgtctggt aaaaacaacc ttctgttagt cgacttcatt
    14341 gagaaaaatg gaaacagcaa cggactgaat atcgatctgg tcagcgcacc aaaaggaact
    14401 gcagtagatg tctttaaagc tacgactcgg agtattggct tcagtgatgt aacatcggtt
    14461 atcgagcaaa agaacgatac agacaaagca acatggactc tgatcggcta taaatctgtg
    14521 gccaacgccg atgcggctaa aaaggcaaca ttactgatgt caggcggcta taaagccttc
    14581 cttgctgagg tcaacaacct taacaaacgt atgggtgatc tgcgtgacat taacggtgag
    14641 tccggtgcat gggcccgaat catgagcgga accgggtctg ccggcggtgg attcagtgac
    14701 aactacaccc acgttcaggt cggtgcggat aacaaacatg aactcgatgg ccttgacctc
    14761 ttcaccgggg tgaccatgac ctataccgac agccatgcag gcagtgatgc cttcagtggt
    14821 gaaacgaagt ctgtgggtgc cggtctctat gcctctgcca tgtttgagtc cggagcatat
    14881 atcgacctca tcggtaagta cgttcaccat gacaacgagt ataccgcaac tttcgccggc
    14941 cttggcacca gagactacag ctctcactcc tggtatgccg gtgcggaagt cggttaccgt
    15001 taccatgtaa ctgactctgc atggattgag ccgcaggcgg aacttgttta cggtgctgta
    15061 tccgggaaac agttctcctg gaaggaccag ggaatgaacc tcaccatgaa ggataaggac
    15121 tttaatccgc tgattgggcg taccggtg
//
LOCUS       DAKISA010000101        14937 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.101, whole genome shotgun sequence.
ACCESSION   DAKISA010000101 DAKISA010000000
VERSION     DAKISA010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14937
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.101"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            312..1610
                     /gene="kgtP"
                     /locus_tag="OVW45_003327"
     CDS             312..1610
                     /gene="kgtP"
                     /locus_tag="OVW45_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCV5527696.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HVNVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1607..1930)
                     /locus_tag="OVW45_003328"
     CDS             complement(1607..1930)
                     /locus_tag="OVW45_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCV5527697.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1976..3331)
                     /gene="pssA"
                     /locus_tag="OVW45_003329"
     CDS             complement(1976..3331)
                     /gene="pssA"
                     /locus_tag="OVW45_003329"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCV5527698.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENSGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLKLAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3445..6105)
                     /gene="pat"
                     /locus_tag="OVW45_003330"
     CDS             complement(3445..6105)
                     /gene="pat"
                     /locus_tag="OVW45_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCV5527699.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGSFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPTLPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6137..6835)
                     /gene="tapT"
                     /locus_tag="OVW45_003331"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6137..6835)
                     /gene="tapT"
                     /locus_tag="OVW45_003331"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCV5527700.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6904..7323)
                     /gene="trxC"
                     /locus_tag="OVW45_003332"
     CDS             complement(6904..7323)
                     /gene="trxC"
                     /locus_tag="OVW45_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCV5527701.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7530..8567
                     /locus_tag="OVW45_003333"
     CDS             7530..8567
                     /locus_tag="OVW45_003333"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCV5527702.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8615..9304)
                     /gene="ung"
                     /locus_tag="OVW45_003334"
     CDS             complement(8615..9304)
                     /gene="ung"
                     /locus_tag="OVW45_003334"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCV5527703.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9609..9992
                     /gene="grcA"
                     /locus_tag="OVW45_003335"
     CDS             9609..9992
                     /gene="grcA"
                     /locus_tag="OVW45_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCV5527704.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10048..10635)
                     /gene="eamB"
                     /locus_tag="OVW45_003336"
     CDS             complement(10048..10635)
                     /gene="eamB"
                     /locus_tag="OVW45_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCV5527705.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10738..11619
                     /locus_tag="OVW45_003337"
     CDS             10738..11619
                     /locus_tag="OVW45_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCV5527706.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIVDVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVAKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            complement(11828..13162)
                     /gene="srmB"
                     /locus_tag="OVW45_003338"
     CDS             complement(11828..13162)
                     /gene="srmB"
                     /locus_tag="OVW45_003338"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCV5527707.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13294..14031
                     /gene="trmN"
                     /locus_tag="OVW45_003339"
     CDS             13294..14031
                     /gene="trmN"
                     /locus_tag="OVW45_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCV5527708.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWLTQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQVGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(14016..>14937)
                     /locus_tag="OVW45_003340"
     CDS             complement(14016..>14937)
                     /locus_tag="OVW45_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HCV5527709.1"
                     /translation="VANLEFNQFPPTALYHPQARNFLLTEALRGEGAYLKRPDGTRFM
                     PDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLGLG
                     IDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSLLE
                     CLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDYVG
                     IVRTTKRLERALRRINMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRKES
                     RGLHFTLDYPELLTHSGPSILPPGNHYINR"
BASE COUNT         3602 a         3781 c         3913 g         3641 t
ORIGIN      
        1 aaaggccatc ctgacggatg gcctttttgc attggcgcag aaaaaaatgc ctgatgcgac
       61 actgcgcgtc ttatactccc acatatgcca gattcagcaa cggatacggt ttccccaact
      121 tgcccacttc catacgtgtc ctccttacca gaaatttatc cttaagctcc tcaataacca
      181 ttttcctgct aactaaattc atggttaagg ttgcacaatg atatgcaaca aatgtatagc
      241 atttccttta ccaaaaaaat aaacaaaagc gaccgacaaa agcatcggat tacggcagga
      301 gacataatgg catggctgaa agtactgtaa cggcagacag caaactgaca agtagtgata
      361 ctcgtcgccg catttgggca attgtggggg cctcttcagg taatctggtc gagtggttcg
      421 atttctatgt ctactcgttc tgttcactct acttcgccca catcttcttc ccttccggga
      481 acacgacgac tcaactacta caaacagcag gtgtttttgc tgcgggattc ctgatgcgcc
      541 caataggcgg ttggctattt ggccgcatag ccgataaaca tggtcgcaaa aaatcgatgc
      601 tgctatcggt gtgtatgatg tgtttcggct cactggtaat cgcctgtttg cctggttatg
      661 aaacaatcgg tacgtgggct ccggcattat tgcttctcgc gcgtttattt cagggattat
      721 ccgttggcgg agaatatggc accagcgcca cctatatgag tgaagttgcc gttgaagggc
      781 gcaaaggttt ttacgcatca tttcagtatg tgacgttgat cggcggacaa ctgctagccc
      841 tactggttgt cgtggtttta caacacacca tggaagacgc tgcactcaga gagtggggat
      901 ggcgtattcc tttcgcgtta ggagctgtgt tagctgttgt ggcgttgtgg ttacgtcgtc
      961 agttagatga aacttcgcaa caagaaacgc gcgctttaaa agaagctgga tctctgaaag
     1021 gattatggcg caatcgccgt gcattcatca tggttctcgg ttttaccgct gcgggctccc
     1081 tttgtttcta taccttcact acttatatgc agaagtatct ggtaaatact gcgggaatgc
     1141 atgtcaacgt ggcgagtggc attatgactg ccgcattgtt tgtattcatg cttattcaac
     1201 cactcattgg cgcgctgtcg gataagattg gtcgccgtac ctcaatgtta tgtttcggtt
     1261 cgctggcagc catttttacc gttcctattc tctcagcatt gcaaaacgtt tcctcgcctt
     1321 atgccgcttt tggtctggtg atgtgtgccc tgctgatagt gagtttttat acatcaatca
     1381 gtggaatact gaaggctgag atgttcccgg cacaggttcg cgcattaggc gttggcctgt
     1441 catatgcggt cgctaatgct atatttggtg gttcggcgga gtacgtagcg ttgtcgctga
     1501 aatcaatagg aatggaaaca gccttcttct ggtatgtgac cttgatggcc gtggtggcgt
     1561 ttctggtttc tttgatgcta catcgcaaag ggaaggggat gcgtctttag tgacgggtca
     1621 gttgccagac ggtatagccg gtgcttgcac cggcgacatc ccaggccaaa tccttccagc
     1681 tccagccgct cccttcgggg cggctatccc aaagctcttt tgacgccccc aaactgacag
     1741 agaacatcaa tccaaacatg gcactgcgat cccggctcat cccctgatgc tgtgaatatt
     1801 catttccggc ggcggaaagc atcgccgagg cgataaagtg ttgagcttta tcctgcccgc
     1861 tccagctatc gttagccata tggctgcatc cagaaagtaa caatagcgaa cagacaaaaa
     1921 gaatacgcaa aacgtggcct ccaaaaaaca aaccccgtac aggacggggt tgtgattaca
     1981 ggatgcggct aattaatcgg tcgatgcgga tacggcgcaa acggcggatg agtttacgaa
     2041 ccttcaccgg ataatcggca atactttgca gatcgcgata gtgcttaacg atggtggtat
     2101 gctcgcggat cagctccagt tctttctctc gctgcggcgc aagcttcagt tgtggatcgt
     2161 ggatcaaaat ggcgttttcc agatccagac gccaggcgcg cgggttcagg ttattaccgg
     2221 tgatcaacat ccacttatca tcaacccaca tccctttcag gtgataggtg ttgtcgtcat
     2281 ctttccataa ccgaaccact agctggtcag tattgacgta atactgcaaa cggctcagga
     2341 aacgacgcag attgatctca tagagataag gcaatgcgcc aattatcttg aaaggttcat
     2401 cttccggaat gtagaagtcg ttcgcggttt tatcaccaac aataatttcg acctttttcc
     2461 cttcgcgcag caactggata atattgcgca caaggattgc tggcaggttg aagtatggcg
     2521 tacagatggt tagtttctgc tcggcacaag gcataagatg gaaaatggtc ttgttcaaca
     2581 gactcgattt ccccagcccc actagcggcg ttacggaaag ctgatcgttg tcggcatcgc
     2641 cctggaaatg ataagcggca tcacgcagct cctggcggaa cagacgaata tcgttcttga
     2701 tttccgggct ttttggccga ttaacatcat ccagacgatt aacgccgcgg ccattcataa
     2761 tattctgtgt aacccattca aacataatgt ctgacatctt acggttacgg ataagatgat
     2821 aacggtcgta gcgatattta tcgtgctgat gcaggtaaac atcgttcagg ctggcaccgc
     2881 tataaagtac gctatcgtcg atgataaagc ctttaaagtg cagaacacca agggcttcac
     2941 gggtattgat tggaacgcca taaaccggaa catctacgcc cgaattttcc tgcgccatgc
     3001 ggcagtacca gtcagcgtta gtgttagatg ccgcagcgcc aatgcgtcca cgttgtgcac
     3061 gatgccagtc gaccagcacc cgcacatcca gttccggacg ctgcctttta gcttcataca
     3121 acgcgttcag aatgcctttg ccaccgtcat cctgttcgag atacagggcg acaatgcaaa
     3181 tgcgctgctt cgcgctggct attttttcca gcagcgtctc ccggaagtcg gcgggagcgt
     3241 aaaagaaatc gacatcatca actgattgag aaatcttggg tagttgggca aggtgttgtt
     3301 gatgtttatt acgcttaaat tttgacaaca tcacagtgca tttcttctct gttcattgaa
     3361 gggtcctctg tgcaatgcag acgacataag cgggcaataa taacaccagt cccgattaag
     3421 tggtcaacat ttccagtacc ttactcatga ttcctcgcgt tgggcaagat ttagcgtaag
     3481 cccaacgatc ccctcttcga gctggatatc aacgttaaac ccgagcttgc gggctagcgc
     3541 caccatgcca cgattgtttg gcatcgtaat accattcaga cgttgtagtc cgtgatctcg
     3601 cgtataggta atcaactttt ccattaagcg tcgacctaag cctaaccctt tgagatccga
     3661 gcgaaccagc acagcaaatt cggcatcgat gttatcagga tcggaaatcg cacgcgtgac
     3721 gccgaggatc tcttccgttt gatcaatacg tcgtaccgct acaaacgcca tttcccgatc
     3781 gtagtcgatc tgtgtcatgt tggctaaatc ttcatgggta aattcgttga tctcgctaaa
     3841 gtagcggtaa taaagatctt ctttggtgac tcgcgaaatg aattgctgaa gttgtggctc
     3901 atcttctggc aaaatcgggc ggaacaagca gcgttcaccg tttttcaatt ctacccattc
     3961 ttccagctga tgcggataag ggcgcactgc cagccgactc tcgttatcgc cttcaaacgg
     4021 cgagatatcc agcgtgacat ccagcgcggt aaattcactg ccagaagcca gcaaaggatg
     4081 aatatccaga cgctgaattt ccgggcaatc gacaatcaag ttggaaacct gcaccagaag
     4141 ctggctcaag cctgcaacat ccaatgggcg tagcgcactg cgcgcacgaa tctttttact
     4201 tttgatcccc tgaataacca gatagcgggc caggttcatg ttcagcggcg gcagtgcgac
     4261 gacggcttga tcttcaggac gccactccac accgccttca cccagcatga tcaacggccc
     4321 gaaaaccgga tcgtgctcaa ccacaacccg caactcctga gcgccagcac ggttagccat
     4381 actttgcacc aacaggccgt ggacccgcgc ctgtggccag gccattttta cgcgatcgaa
     4441 aatagcgttc gccgcttgct ggacttcatt ggctgtacgc aggtaaagca tgacgccctg
     4501 aacttccgat ttatgtggaa tatccggcga acgcaatttc agcgccaccg gataaccaat
     4561 ctgttcggca atatgcaccg cttcggtgct atcgctggca atccaggtag ggagcgtgtt
     4621 catgccatac gcttgcagga tgggctgaac ttcatgggta tcgagcgacg tagccccttc
     4681 ggcaatcgcc tgttgcaaca gaagatgcgc ttctgcggta ttggaagtca gattgctggg
     4741 caacgtcggc gtttcgcgta gttgcttctg attacgccgg tactccacca tatgcataaa
     4801 agcagtgatg gttccttccg gggtacggta ggtcggcagc ccggcttcgc tgaataaacg
     4861 tcgtgcctct tgcgaggagt gctcgccgca ccagttcgtc agcaaagaga catatttgct
     4921 gcggggatga tgctttaccg cttcaattaa tacttgcgcg ctttctgttg cgggagcagc
     4981 ggcgctgggc gaatgaataa ccatcagcgc atcaaaatcc tggctgtgga gcagaatgtc
     5041 cagcgttttg acataatgct cgctgctggc gtcatcgcgc agatcgagag ggttagagac
     5101 tgccacatgt tctggcaatg catcgcgcaa tttctggcag gtttcttcgc ttagcgttgc
     5161 cagcttgcca ttgcgtgacc ataaggcatc cagcgccagc gcggcaggcg cagcaccgtt
     5221 gctgataatc atcaaccggt cgccacgcag cgggcgcata tggctaaggg tttccaccgc
     5281 cgaaaacagt tcgtgggtat cctgtacccg caacaaaccg gcacgctgaa tagccgcatc
     5341 ccatgccggg tccattcctg ccgtcgtgtt gagcagtcgc tgtgccgccg ggctacgtcc
     5401 gcttttaatc accagaatcg gtttattacg cgaggcacta cgggctgccg aaacaaagcg
     5461 tcgcgcgtcg cttaactgtt cgagatagag caggatggcg ctggttttac tgtcgcgcgc
     5521 cagatagtcg agcaattcat caacgtcgat atccaggctg tcgccgagcg caataaagta
     5581 ggaaaagccc atctcacgct gttgcgccca gtcgaggatg gtgttggaga cggctgccga
     5641 ttgtgaaata aacgccagct tgccgcgttt aatcggcaca ggcgaaaagc tggcattcag
     5701 accttgccag ggagccagta aacccagact gtttggtcca agcaggcgca tgttatggcg
     5761 cagggcgcag gcgcggagat cttcgtgttg cgatgccggg gcggaaagaa taatgcaggt
     5821 tttacagcct ttctcgccga gctcttccag aagagcaaga ttacggctgg cattggtaca
     5881 taaaaccgca aggtcgggtg taaagggcaa gctggcaata tccggccagg ccaacacacc
     5941 caacaccgct ttccaggctg gcgtcaccgg gagtaccggt ccgttaaagc ttcccgccag
     6001 caggttacgc atcatcaggt aacctgcgcg attgggtttc atcgacgcgc caattaccgc
     6061 tatcgatttt ggtcgcagta gtgcttccag tcctcgctga ctcataccgg tctcccgcag
     6121 aagtgaccga atgattttaa acgctttcta actgttctgc tgtgatgcta cccagatgtt
     6181 gcgtttttcc tgccagatag cgtgttttaa agcgggtaaa atgctcgcct aaccctgctg
     6241 ccgccccggt atcgccggcc atatctaaca gtgcgatggc tacctcggca gtacaatatt
     6301 ggcctgcagc ctgggcttca cgcaggcgat aggcagaaag ccgggaaaga tcgacggaaa
     6361 tgacgggaag attatccaga tacggacttt tacgaaacat cttgcgagct tccggccagg
     6421 taccatcgag catgataaac agcggtggct taccggcagg tggtgtgaag atcacttccc
     6481 gttgctcatc agcatacgag gcgggaaaga ccaccattgg ctgataatcc gggttttgta
     6541 ccagatccag caaatcctgc gagggttcgg tacgcgacca ttgaaacgca acggtatcag
     6601 gcaaaatatc agcaatcaga cgcccggtat tactgggctt cattggctcg gtgtcgaaca
     6661 tcagcaaaca gaagcgactt tttgcttgtg ctggggtaat tgtcgaacag agacataatt
     6721 tctctggcaa aagacagcgt tggcagcgac gaacgcgatt accgcgggca agaaaaggac
     6781 gtgttgcgcg cgcaatacgc tcggcgcgta actggagaac agcgttttcg gtcataagag
     6841 agcgtcgaaa aaacgccatt gtcgcagagg agaaaacggg gcacaagatg caccccggta
     6901 agattaaaga gattcgttca gccagctatc gaacggcgct ttcggtactg cgccattaag
     6961 catgtcgaca acctgaccgt ttttgaaaat catgatcgtc ggtatactac gaattccaaa
     7021 gcgactgctc aattcacgtt cagcttcggt attcactttc acaaagcgca ctttaccgct
     7081 acgctcttgc gcgacatctt caaaaattgg tgcgaaatta cggcaggggc cgcaccacgg
     7141 tgcccagaag tcgatcacca caggtagatc atccttcagc aatttgtcga gtgtttcacc
     7201 ggtcgcatta atcacctctc cgtcaaacaa gtcgtgaccg cagcgtccgc attttgccgc
     7261 atcttcgatc cgatcgtcgg gaatgcgatt gatggcctga caatgggtac aaacggtatt
     7321 cataactaac ctcgggatga gtaaggagac ttatcggcaa cgacgccggt atgtttctaa
     7381 tatgttacat attatcgggg acattgttcc agacaacaat ctgttttatt caataggtat
     7441 gatagtcgca ggctttggta cgaaatcatg gcgaaccgcg cggacttcgg gtaatctgcg
     7501 cgcttcgcgc agcgctggtg gagaaaagca tgaacgatga aatgaaaggt aaaagcggca
     7561 aggtcaaagt gatgtatgtc cgcagtgatg atgattctga taaacgtacc cacaacccgc
     7621 gtaccgggaa agggggcggg cgtccaggaa aatctcgtgc tgacggtggc cgtcgccccg
     7681 cccgcgatga caaacagagt cagccccgtg accgcaagtg ggaagattcg ccgtggcgca
     7741 cggtttcccg cgcgccgggt gatgagacgc cggaaaaggc cgatcacggt ggcatcagtg
     7801 gtaaaagttt tattgatccg gaagtgttgc gtcgtcagcg tgcggaagaa acccgcgtct
     7861 acggcgaaaa tgcctgtcag gcactgttcc agagccgtcc ggaagcaatt gttcgcgcct
     7921 ggtttatcca gagcgtaacg ccacgtttta aagaagcctt gcgctggatg gcggcaaacc
     7981 gcaaagcgta ccatgtggtg gatgaagctg aattgacaaa agcgtcaggc acggaacatc
     8041 acggcggcgt ttgcttcctg atcaaaaagc gtaacggtac aaccgtgcag cagtgggtaa
     8101 gccaggcagg cgcgcaggat tgcgttctgg cactggaaaa cgaatctaac ccgcataacc
     8161 tgggtggcat gatgcgcagc tgcgcgcact tcggcgtgaa aggtgtcgtg gtgcaggatg
     8221 cggcactgct ggagtcgggg gcggctatcc gtaccgcaga aggcggcgca gagcacgttc
     8281 agccgattac tggcgacaac attgttaacg tgctggatga tttccgtcag gcaggttaca
     8341 ccgtagtgac cacttccagc gagcagggta aaccgctgtt caaaactagt ctgccagcga
     8401 aaatggtact ggtgctgggt caggaatatg aagggttacc ggatgccgca cgcgatccga
     8461 acgatctgcg cgtgaagatt gacggtacgg gcaacgttgc cgggctgaat atctctgtcg
     8521 caaccggcgt tttgcttggt gaatggtggc gtcagaataa agcctgataa atcagccggg
     8581 tggcaactct gccatccggc atttccccgc aaatttactc actctctgcc ggtaatactg
     8641 gcatccagtc aatcggcgtc tcgccacgtt gttccagcca ctgatttgcc agcacaaaat
     8701 ggttgcagcc aaagaatcca cgatgcgccg aaagcggcga cggatgcggt gctttcagta
     8761 catgatggcg ttgcttatct ataatcgccc ctttcttttg cgcatgtgat ccccacaaca
     8821 aaaacaccac gccttcgcgg tgctggttaa tcaggctgat gactttatca gtaaacgtct
     8881 cccaaccgag gctggcgtgg gaatgcgcct gacctgcgcg aaccgtcaac acagtattga
     8941 gcagcagaac gccctgacgc gcccagcttt cgagatagcc atgattaggg cgggtgaagc
     9001 ccggaatagt attttccagc tctttataca tattcaataa tgacggagga atggcaatgc
     9061 cgggacgaac ggaaaatgcc agaccatgcg cctgtcccgg tccgtgataa ggatcctggc
     9121 cgagaatcac cactttaacg tcacccaact cggtaaagcg gaacgcgtta aagacatctt
     9181 tttgtggtgg atagatagtg acgccggact gccgctcgct ggcgacggtc tgaagggtat
     9241 taagaaaata aggttgttgc ttctcttcag ccagcacgtc atgccaggtt aattcgttag
     9301 ccatctcgct ctcctgcgaa tcttcaatcc gcctagctta actgcttctt tctacagaac
     9361 aaaatcatgc tcatttttta gacttaaacg agtgcaaaaa aatgtaaatt ggtaaaattt
     9421 cacacccgca taagttgatg taaaacaata aaatccattt acgaccacaa tcgatgtgtg
     9481 gtttttattg atttaaatca aagaatcaag ggtggttgag gagtatatat acactcaagc
     9541 aacaatggtt ttaccaattg gccgcgacag gctgaacaaa tcaaataatt ttgccgggga
     9601 ggcatcacat gattacaggt atccagatta ctaaagccgc taacgacgat ctgctgaact
     9661 ctttctggct gctggacagc gaaaaaggcg aagcacgttg catcgttgca aaagcaggtt
     9721 atgcagaaga tgaagtggtt gcagtaagca aactgggtga cattgaatac cgtgaagttc
     9781 cagtagaagt gaaaccagaa gttcgcgttg aaggtggtca acacctgaac gttaacgttc
     9841 tgcgtcgtga aactctggaa gatgcagtta agcatccgga aaaatatccg cagctgacca
     9901 tccgtgtatc cggttatgca gttcgcttta actctctgac tccggaacag cagcgcgacg
     9961 ttatcgctcg tacctttact gaaagcctgt aaggcttaag tagcaagtta ctaagcggaa
    10021 gagggcgacc tcttccgctt tttttcgtta atagaaaatg cgtaccgcgc aatagaccag
    10081 taacagggca agcacgatat ttaactggcg accatactgg cgaaacaatc gctgaaacag
    10141 atgccccgcc agcgcccagc acacattgcc aaacgtccca atcatcgcca gcaaaacgct
    10201 gacgccaact acccagctta gcgcctgtgt ttgcggcaga acaaacgtcg acagtgccgt
    10261 aacaccgtac aaaatgattt tgacgttcac aaactgcaaa gcaaagctgg cccaaaagct
    10321 gattggtttt gcctgaagtc cgtcttcctt tgttgggctg gtggcgattt tccacgccag
    10381 ccagacaata tatgccgccc ccgcccaact caaaaggtgt accgctgccg ggtcaatcac
    10441 tgccagtgaa aatgaaatgc ccgcacacag taacatcaca atcaaaaatc ccagactcat
    10501 ccctgccagc acgcgggtac tttgacgaaa tccatgcgac gtagcagagc taagggcgag
    10561 aatattgttc ggtcctggcg tcatagcggt aatcagggtg taagtccaaa aagcacttaa
    10621 aagggtcggt gtcacttgtt atctcctctg ttttggtaga cagtacccga cacggaacat
    10681 ttaaaaaaat gaatgttttt atattctaga ttcgatattt tcgataggtt tggggttatg
    10741 gatctgcgcc gttttattac gcttaaaacc gtggtggaag agggttcctt tttgcgagct
    10801 tcgcaaaaat tgtgctgtac acaatcgacg gtgacttttc atattcagca gcttgagcag
    10861 gagttttcag tccagttatt tgagaaaatt ggccgacgga tgtgccttac ccgcgaagga
    10921 aaaaagttgc tgccgcacat ttatgaactt acccgagtga tggatacgct tcgtgaggct
    10981 gccaaaaagg agtcagatcc ggacggggaa ctacgcgttg tttcgggcga aacgttgctc
    11041 tcttaccgaa tgccgcaggt gttgcagcgt tttcggcaac gcgcaccaaa agtgcgttta
    11101 tcgttgcagt cgctgaactg ttacgtgatc cgtgatgcgc tactgaatga tgaggccgat
    11161 gtcggcgtct tttatcgcgt agggaatgat gatgccctga accgacgaga gttgggtgaa
    11221 caatcgctgg tgctggtggc ttcaccacaa attgtagatg tcgattttac tgagccggga
    11281 agacataacg cctgtagctt tattatcaat gaaccacaat gtgtcttccg gcagatattt
    11341 gagagcacgc tgcgtcagcg gcggatcacg gtggaaaaca ccattgagct tataagtatc
    11401 gaaagcatca agcgttgtgt cgcggcgaat atcggagtca gctacctgcc gcgttttgcg
    11461 gtggcaaaag agttggagtg cggagaatta attgaattgc cttttggcga acagtcgcag
    11521 accattacgg ccatgtgcgc acaccatgct ggaaaagcgg ttagcccggc gatgcacact
    11581 tttattcagt gtgttgaaga gagttttgtg gctggataaa aataatgtcg ggattaaacc
    11641 cggcatttaa cttgtcgaca aagtgcgctt tattcatgcc ggatgcggcg tgaacgcctt
    11701 atccggccta caagatcgtg caaattcaat atattgcatg agccacgtag gcctgataag
    11761 cgtagcgcat caggcagttt tgcgtttgcc agcactttta ataccaggct taaacccggc
    11821 attgagatta ctcttctgtc gtttgcggtg gcacgcccgt tccgcttggt ttacggcgct
    11881 taccaatatt tttggtgtcg cgatggcgtt ttttcacccg cggtttctct ttctctttag
    11941 cttttttctt ctcggcgcgt tttgccagta ctttcttcga tggcttgccg gtctgcttct
    12001 cgcttggcgc acgcgttttc gggcgtaact catcaataac gcgagcttta atcggctctt
    12061 caatgtaacg gccaaccttg cccagcagca aatggtcatg ggcttccacc agcgaaattg
    12121 cggtgccttt acgaccggcg cgcgcggtac gtccgatacg gtgcaaataa gtatcgccac
    12181 tgcgcggcat atcgaagtta aagacgtggc tgacgtcagg aatgtcgata ccgcgcgcgg
    12241 caacatcggt tgcaaccagt acgtttacgc gaccttcggt caaacgcttg atcgcttcgt
    12301 tacgcttgcc ttgtaccatc tcaccttcga gatagcagtt gttgatgccc gcttcgcgca
    12361 gccagtttgc cagctcatgc acacgttcac gcttacgcac aaacacaatt gagcgggtcg
    12421 cttccggctg ttttaacaga tgcaccagca atgcggtttt atgttccagg tcatcggcgc
    12481 ggtaatacca ctgatgaatt tttttgcgct cacgggtgga cggattggca gaaacctcca
    12541 ccggatcttc cagcagacgc tcggcaaagt cctgaatcgc atcgccttcc agcgttgccg
    12601 aaaagagcag ggtctgttta cgccagcgcg tttcgccagc aatatgttcg atatcctgag
    12661 cgaagcccat atccagcata cggtctgctt cgtcgaggat cagcgtttca accgcgcggc
    12721 aatcgaagtt ctcttctttt atgtattgca gcagacgtcc ggtcgtggcg accacgatgt
    12781 cctgattttc gctgaacact tccgcgtggt tcatataggc tacgccgccg gtgatggtgg
    12841 cgatatccag atgcgtatgt ttcgccagtt cgcgggcatg atcggccacc tgcatcgcca
    12901 gctcgcgagt tggggtgagg atcaaaatac gcggcggacc ggatttctta cgtgggaaat
    12961 cgagcaggtg ctgcaacgct ggcagcagat acgccgccgt tttaccggtg cctgtcggcg
    13021 cagaaccgag tacatcacgg ccatcgagcg caggcggaat ggcggcagcc tgaatggcgg
    13081 tcgggcgagt gaaaccttta tcctggaggg cttccagcag gctttcgtcg agttcaagtt
    13141 cggaaaaagt cgttacagtc atgttctacc tctgtgtggg gcgctgatta tagacgttac
    13201 ggctgcaatc ttcatctgtt tgtatggata tcgcttctcg ggtattgttc tcacccggtg
    13261 ctatcgccgt ttttcctgca aggttttatt ttcatgtcac agtctacatc cgtgcttcgt
    13321 cgtaatggat ttacttttaa acagtttttt gttgctcacg atcgctgtgc gatgaaagtg
    13381 ggaacggatg gcattttgct gggcgcatgg gcaccggtgg ctggggtgaa acgttgcctt
    13441 gatatcggcg cgggtagcgg gttgctggca ctgatgctgg cgcagcgaac cagtgacagc
    13501 gtgataattg atgccgttga gctggaaagt gaagctgccg cgcaggcgca ggaaaatatc
    13561 aaccagtccc cgtgggcaga gcggattaac gtccatacgg cggatattca gcagtggctc
    13621 acacagcaga cagtacgctt cgatttaatc atcagtaacc caccttacta tcagcaggga
    13681 gtagagtgcg cgacacctca acgggaacag gctcgctata ccactacgct tgatcaccca
    13741 tcgctgctga cctgtgcggc ggagtgcatt accgaagagg gatttttctg cgtggtgctg
    13801 ccagagcagg ttggtaatgg ttttactgaa ctggcattaa gcatgggctg gcatttacgt
    13861 ttgcggacgg atgtggcgga aaacgaagcg cgactgccgc atcgggtgct gctggcattc
    13921 tccccgcagg cgggagaatg ctttagcgat cgcttagtca ttcgtgggcc agaccagaac
    13981 tattccgaag cgtatacggc gctgacccag gctttttatc tgtttatgta atgattgccg
    14041 gggggaagga tcgatggacc ggaatgcgtg agcagttccg ggtaatccag cgtaaaatgc
    14101 aggccacgac tctccttgcg catcatcgca cagcgaacaa tcaactcggc aacctgtacc
    14161 agattacgca gctccagcaa attattcgac acgcggaagt gggcgtaata ttcgtctatt
    14221 tcctgttgca acatattgat acgccgcagg gcgcgctcaa gacgttttgt tgtgcgcaca
    14281 atgccgacgt aatcccacat aaacagccgt agctcgtgcc agttatgctg aattactacc
    14341 cgttcgtcag ggttctcaac gcggctttca tcccacggtg gtaacgtact gacgccgtgg
    14401 gcatcaggca tacgtctggt gatatcttct gccgccgacc agccatagac cagacactcc
    14461 agcaatgaat tcgaggccat gcggttagcg ccgtgtaagc cggtataact cacctcgcca
    14521 atggcataca agccctcgac gtccgtacgc ccatgatcat caaccattac accaccgcag
    14581 gtatagtgtg cagcaggcac aatcggtacc ggttcttgtg tgagatcaat ccccagcccg
    14641 agcaattttt cataaatcat cgggaaatgc tggcgaataa aatcggcggg cttatggctg
    14701 atgtcgagga acatacaatc tgcgccgagg cgtttcattt catggtcaat ggcgcgggcg
    14761 acaatatcgc gcggggccag ttcgccgcgc tcatcaaaat cgggcataaa acgcgtgcca
    14821 tccgggcgct tgagataagc gccttcgccg cgcagtgctt ccgttaacag gaaattgcgt
    14881 gcctgtgggt gatacagtgc ggtagggggg aactgattaa attcgagatt ggcaacc
//
LOCUS       DAKISA010000102        14787 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.102, whole genome shotgun sequence.
ACCESSION   DAKISA010000102 DAKISA010000000
VERSION     DAKISA010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14787
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.102"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..454)
                     /gene="adeQ"
                     /locus_tag="OVW45_003341"
                     /gene_synonym="yicO"
     CDS             complement(<1..454)
                     /gene="adeQ"
                     /locus_tag="OVW45_003341"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCV5527710.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITS"
     gene            629..2395
                     /gene="adeD"
                     /locus_tag="OVW45_003342"
     CDS             629..2395
                     /gene="adeD"
                     /locus_tag="OVW45_003342"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCV5527711.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACSLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDVEKFAFTTLEVTE"
     gene            complement(2440..3831)
                     /gene="uhpT"
                     /locus_tag="OVW45_003343"
     CDS             complement(2440..3831)
                     /gene="uhpT"
                     /locus_tag="OVW45_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCV5527712.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3969..5288)
                     /gene="uhpC"
                     /locus_tag="OVW45_003344"
     CDS             complement(3969..5288)
                     /gene="uhpC"
                     /locus_tag="OVW45_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCV5527713.1"
                     /translation="MLPFLKAPADAPLMADKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(5298..6800)
                     /gene="uhpB"
                     /locus_tag="OVW45_003345"
     CDS             complement(5298..6800)
                     /gene="uhpB"
                     /locus_tag="OVW45_003345"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCV5527714.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLVLP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6800..7390)
                     /gene="uhpA"
                     /locus_tag="OVW45_003346"
     CDS             complement(6800..7390)
                     /gene="uhpA"
                     /locus_tag="OVW45_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCV5527715.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(7465..7755)
                     /gene="ilvN"
                     /locus_tag="OVW45_003347"
     CDS             complement(7465..7755)
                     /gene="ilvN"
                     /locus_tag="OVW45_003347"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCV5527716.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7759..9447)
                     /gene="ilvB"
                     /locus_tag="OVW45_003348"
     CDS             complement(7759..9447)
                     /gene="ilvB"
                     /locus_tag="OVW45_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCV5527717.1"
                     /translation="MASSGTTLTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDVATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPSPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(9553..9651)
                     /gene="ivbL"
                     /locus_tag="OVW45_003349"
     CDS             complement(9553..9651)
                     /gene="ivbL"
                     /locus_tag="OVW45_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCV5527718.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            10216..10305
                     /gene="tisB"
                     /locus_tag="OVW45_003350"
     CDS             10216..10305
                     /gene="tisB"
                     /locus_tag="OVW45_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCV5527719.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            10585..11769
                     /gene="emrD"
                     /locus_tag="OVW45_003351"
     CDS             10585..11769
                     /gene="emrD"
                     /locus_tag="OVW45_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCV5527720.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11777..12274)
                     /locus_tag="OVW45_003352"
     CDS             complement(11777..12274)
                     /locus_tag="OVW45_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCV5527721.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRCDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(12271..12633)
                     /locus_tag="OVW45_003353"
     CDS             complement(12271..12633)
                     /locus_tag="OVW45_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCV5527722.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(12623..12970)
                     /locus_tag="OVW45_003354"
     CDS             complement(12623..12970)
                     /locus_tag="OVW45_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCV5527723.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            13079..13528
                     /locus_tag="OVW45_003355"
     CDS             13079..13528
                     /locus_tag="OVW45_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5527724.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFVGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(13777..>14787)
                     /locus_tag="OVW45_003356"
     CDS             complement(13777..>14787)
                     /locus_tag="OVW45_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCV5527725.1"
                     /translation="ETQWLKDAPSQPLQQSLKDLERAYKNFFQNRAAFPRFKKRGQND
                     VFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWYISIQTESE
                     VSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQLSRKVKFSN
                     NWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSKSAAGTVSL
                     PGRNVRAKSGLNRSILDQGWYEMRRQLAYKQLWRGGQVLAVPPAYTSQRCVCCGHTAK
                     ENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQA
                     TA"
BASE COUNT         3507 a         4082 c         3826 g         3372 t
ORIGIN      
        1 cgctggtgat accaatacgt aaacttaagg gaatgttgga gatcatccag taccggatac
       61 gaaacagcgt gagcaaaaat agtccaactg cgccccagaa tattgcgccc atcccggtct
      121 gccaggagat gcccatcgcc cccacgacca cgaaggcaaa gaaggcgttc agccccattg
      181 ccggagccag cgccacgggt aagttagcaa atatccccat cgcaatactg ccgataccgg
      241 caatcaaaca ggtggtaaca aacaccactt tcgggtccat ttgtgccgcg ccgaggattt
      301 gcgggttcac aaaaacgatg tacaccatgg ttaaaaaagt ggtcatcccc gcaatcaatt
      361 ctgtgcggac ggtggtccca tgctgaggta gtttaaataa tcgcgaaagc gtccctgatt
      421 cattactcac gtaatcggta ttgtcattat tcattttttt gtccattgga ggagatttaa
      481 tcccttgctt ttgtaaaaca aatgacaaca acggttcagt gataattatc acatttcaat
      541 tgcacattaa tggatattct ttaataatct cgcgacgttt ctttatgata aataataatc
      601 aaattgataa aatcaaaatg agaaaaatat gaataattct attaaccata aatttcatca
      661 cattagccgg gctgaatacc aggaattgtt agccgtttcc cgtggcgacg ctgttgccga
      721 ttatattatt gataatgtct ctattctcga cctgatcaat ggcggagaaa tttccggccc
      781 aattgtgatt aaaggacgtt acattgccgg tgttggcgca gaatacgctg atgctccggc
      841 tttgcagcgg attgatgctc gcggcgcaac ggcggtgcca gggtttattg atgctcacct
      901 gcatattgaa tccagcatga tgacgccggt cacttttgaa accgctaccc tgccgcgcgg
      961 cctgacgacc gttatttgcg accctcatga aatcgtcaac gtgatgggag aagccggatt
     1021 cgcctggttt gcccgctgtg ccgaacaggc aaggcaaaac cagtacttac aggtcagctc
     1081 ttgcgtaccc gccctggaag gctgcgatgt taacggtgcc agttttaccc ttgaacagat
     1141 gctcgcctgg cgggaccatc cgcaggttac cggccttgca gaaatgatgg actaccctgg
     1201 cgtaattagc gggcagaatg cgctgctcga taaactggat gcatttcgcc acctgacgct
     1261 ggacggtcac tgcccgggtt tgggtggtaa agaacttaac gcctatatta ctgcgggtat
     1321 tgaaaactgc cacgaaagtt atcagctgga agaaggacgc cggaaattac aactcggcat
     1381 gtcgttgatg atccgcgaag ggtccgctgc ccgcaatctc aacgcgctgg caccgttgat
     1441 caacgaattt aacagcccgc aatgcatgct ctgtaccgat gaccgtaacc cgtgggagat
     1501 cgcccatgaa ggacacatcg atgccttaat tcgccgcctg atcgaacaac acaatgtgcc
     1561 gctgcatgtg gcatatcgcg tcgccagctg gtcgacggcg cgccactttg gtctgaatca
     1621 cctcggctta ctggcacccg gcaagcaggc cgatatcgtc ctgttgagcg atgcgcgtaa
     1681 ggtcacggtg cagcaggtac tggtgaaagg cgagccgatt gatgcgcaaa ccttacaggc
     1741 ggaagagtcg gcgagactgg cacaatccgc tccgccatat ggcaacacca ttgcccgcca
     1801 gccagtttcc gccagcgact ttgccctgca atttacgccc ggaaaacgct atcgggtcat
     1861 tgacgtcatc cataacgaat tgattacgca ctcccactcc agcgtctaca gcgaaaatgg
     1921 ttttgatcgc gatgatgtgt gctttattgc cgtacttgag cgttacgggc aacggctggc
     1981 tccggcttgt agtttgcttg gcggctttgg actgaatgaa ggtgcgctgg ctgcgacggt
     2041 cagccatgac agccataata ttgtggtgat cggtcgcagt gccgaagaga tggcgctggc
     2101 ggtcaatcag gtgattcagg atggcggcgg gctgtgcgtg gtacgtaacg gccaggtcca
     2161 aagtcatctg ccgttgccca ttgccggcct gatgagcacc gacacggcgc agtcgctggc
     2221 ggaacaaatt gacgccttga aagccgccgc ccgtgaatgc ggtccgttac ccgatgagcc
     2281 gtttattcag atggcgtttc tttctctgcc agtgatcccc gcgctaaaac taaccagtca
     2341 ggggctattt gatgtcgaga agtttgcctt cactacgctg gaagtcacgg aataataaaa
     2401 aagcccggcg tcatgccggg caaaagtcac cagttacgtt tatgccactg tcaactgctg
     2461 aatttttttc tcgcggcgga ttttgcgttc ttccatcacc gccactatcg ccatcaggca
     2521 gatacaacca atcgcggcga tatcaagtgc ggcgaaggtg cctgcccagc cggtaaggcc
     2581 gaataccggc gtcccatcgg caatcattcc cagacctaac ttggcaaagc tgtcaccaat
     2641 caggtaagca aaggtgcctt taataccatc ggcagcgcca atcgcttttt taggtacaaa
     2701 gccaacagca gccacaccaa tcaacaattg cgggccaaag accaggaaac ccaacgcaaa
     2761 gagagaagcc agatagatat attcgttact ggcatgttga tacacaccga gcgtggcgat
     2821 aatcagcgcc agcgcgatgc aggccaccag gccacggcga ccgttcgcca ggtcagagag
     2881 ccagccccac agcagcgtac cgaccagcgc accagcttca aacagcgtaa agccctgaat
     2941 cgccaccgct ttagagagtt tcagttcctg gaacgcgtat acggttgacc actggtcgat
     3001 accaatacgt accacataga ggaaaatgtt ggcgaagcac agcagccaga tcactttgtt
     3061 tttcagcaca tactcaacaa agatctgcca cttggtcata tcggtagatt ctgtctcttt
     3121 gtcctcttcg ctgatctcct cgccgaacag ttcttcagct ttgccgaggc cataagattc
     3181 cggggagtcg ctgccgtaac gcaggccgat aaaaccgaca atcagcgcga taatcgacgg
     3241 gaagataaac atgccgatga catggccatc gaacaggtaa tttgccccga acagcgccac
     3301 gcctgctgcg cctgcaccgc caaggttgtg agaaatattc cagaaaccga ggaatgtccc
     3361 gcgtttacga cgcggcgtcc atttggtgat ggtggagtaa ctgcacgaac cgccggtact
     3421 ctggaaaaag ccgcttaagg cgtagaaggc aatcatcagg aacaggctaa ccgagccgct
     3481 gcccatactg gcactgaagc ccagcataca aatggcagac aggatcagca tgaacggcag
     3541 gaattgtttg gtgtttttgc cgtcggcgta gtaggaaacc agcgttttac ccacgccata
     3601 agtgatggag aaacccaggc cgatcatccc cagctgcgtc atgctcaacc cgtaggtcga
     3661 gatcatgtcg ttctgcgcga tgttaaagtt cttgcgaatc aggtacatcg tcaggtagcc
     3721 gataaagacc accaggtagg attgcatgaa cggtttgaac cacattttgc gccgcacttc
     3781 gagcggaagg tccagggtcg gcttgcgaac ctggtttaag aaagccagca tgggttactc
     3841 ctgaaatgaa tacctgcgtg aggcatgcat tgtaaaaatc agcagcaaat caggcctgag
     3901 atagcgtcca ggcaaaacct ggaaaatttc ttagttttgc cggatatgaa gtgaaaaggt
     3961 gagatgcatc acgcttcgcg cggtgtctgg gcgttcaaaa agggcagtaa cagcagtgcg
     4021 gaaatcccgg cggcgataga gataaccaca aaaaatccgc tccagtgcca ggtatcgagt
     4081 actttcgcca gcggccaacc agcaagcgac gcccccagat aagcaaacaa gccgacaaac
     4141 cccgtcgccg cccctgccgc ctctttgtgg gaacactctg ccgccgccat accgattaac
     4201 atctgtgggc caaagacaaa aaaaccaatg gtgaagaagc aggttgcctg catcacgtag
     4261 ctggcaaatg gcatcagcca cagggagccg actgaaagca aaattccggc ggcgaaaatc
     4321 aaattcatcg gccctcggtt gccgttaaac aatttgtccg agccccaacc cgctaccagc
     4381 gcaccgataa atccacccag ttcaaacatc gtcactgccg tattcgccgt gaccagatcg
     4441 acgcccagcg tctcggacat atacaaattg ccccagtcgt tgatcgccgc ccggaccaca
     4501 tagaccagca catagcaaaa cgaaagcagc cagatatacg gattcagcaa cacatatttg
     4561 gtgaggatct ctttacgcgt cagccctgcc ccttcttgtt gttgagctat ctccagcgcg
     4621 tcgtgtcgcc attcaccgac cgccggtaaa cctaacgcct gcgggcgatc gcgcagccgc
     4681 cagcagagaa aaattcccac gacgatcgcc atacaaccag caatcatcat cccggcacgc
     4741 cagccgtaat gtagtgcagc cgctgccatc acaatgggaa tgagtgcgcc gccgacgtta
     4801 tgcgccgtgt tccataatgc ccaccaacca ccgcgctcgg tacgtgaata ccaggccgtt
     4861 aacagacgcg cacacaccgg tgaaccccag ccctggaaaa aggcgttcag cacccagagt
     4921 acggcaaacg cccatagcga cgtcgagaag ccaaacagaa tgttgataat gcccgtggcg
     4981 ataagcccta tccccataaa ataacgggca tttgagcgat cgctgacaat gccggagaca
     5041 aacttcgaca cgccataggt aatgtaaaac agggtcgcta acaggccgat atcgctacgg
     5101 ctgagcacgc cgttagcaag gatttctggt acggcggcgt taaaactttt ccgcgtgaag
     5161 taaaacagcg cgtaacccag ccagatggtc agcagaatat gccgacgcca gtagcgatag
     5221 cgggcatcaa tttcatattt atcagccatt aatggcgcat cggcaggcgc tttcagaaac
     5281 ggcaacatca tcaaacctta gacatagcgt tgaggtagag aaacgctgac acgcgtgccg
     5341 tgcagacagg aaatgtgtaa tgtgccaccc agcgccgtta cgcgctcgcg cattccggtg
     5401 aggccaaaac cttgttgccc ggaacccggc ggcaaaccgc tgccatcgtc ttcaataacc
     5461 agcatcaacc gttcatcctg ctgccagcct tgcagggtga ccgcgctggc atcagcatgt
     5521 ttcacaatgt tgttcagccc ttcctggcag acacgaaaca gcgtcacgcg ctggttttcg
     5581 cttaacgctg attcatcgat tcgccattcg agatggctga caataccgcg cccttccagc
     5641 tccatttccc gcatcagtga gcggatggcc tgctccaggg tgagatcatc caactggcgc
     5701 ggacgtaacc gacccaacaa acggcgcacc gcgtcgtaaa cgcccagcga tagttgttcg
     5761 atgagttgcc cgctctgctt cacgctggcg ttatctgccg ccagccgctg aacaatgccc
     5821 gcctgagtac gaatagcagt gatggtctgg ccgatatcat catgcagctc acgcgccaca
     5881 tcacggcgca cgctctcttc ggtttccagc aaccgttcag ccagatgctg attgcgcgcc
     5941 agttcctttt gcagcgactg gttaagttca cgcaaccgct ggatgccagc gccaagcaac
     6001 aaccctgtca gactttgcac cagcagcgag agcaataaat ccaccggatg atcgcgccag
     6061 gtttgactgg cgatcagcgc gatggcgttc atcaacgtcg caatcagcgc cccttgccaa
     6121 ccatagtgcc aggccagcgc gataatcggc agcaccagac agaatggcgt aaagcgcgac
     6181 agttcgtccg gcaatcccaa ctggagccag agactgataa caaacagcag caagtaccag
     6241 accagatgtc gcccgcgcca gttgattggc tgagaaacca gtgacgggcc gagcggcagc
     6301 caggtgttat tggcgagata gtgccagaac accagacata tcggggccag cgtcaggccg
     6361 ccagttaaag tcagcaacag cgcattccac gactctttgc cgtgccaaag ccagggcagc
     6421 gactgcaaca acgccgccgc cgttaacgcc gccccctgta acagcaaggt gcgccagtca
     6481 cgctgatggc gatagcgcga gatcagcgct acgggcagta acgtcagtaa actaccgatc
     6541 atcaataacg gaaaatgggt taaaccgacc gcctgcgtta gccagtaaat cagcagccac
     6601 tccgcaccca gcaatacggg ccagtatccg cgcgggcatt gcagcattag ccccagacgc
     6661 agaccaaacg gaaataacag caccgccata tcagggcgct caaccagatg caggctgata
     6721 ctccacaggc aaaaccatgc ggcagagaag ataaaaaagc aggcaataac ggtaattaag
     6781 cgggagaaca acgtcttcat caccagccat caaacatgcg gcgcgccagc tctacgtcgt
     6841 tactgacgcc cagtttttcc atcagattgg cgcgatggac gtgtaccgtt ttcggtgaca
     6901 agcccagttc ggcggcaatc tctttcaccg ccattccttg cgccagtttt tccgccacct
     6961 ggcgttcacg tttggttagc gggtcctgac gaccggatgc cagtttaatg gcaatatccg
     7021 gcgtcagata acagccaccc gtggcaaccg tatgcaccgc agcaatgagt tcatccgggc
     7081 tacagcgttt ggaaagaaag ccgcgtgccc ccgcgttaag cgcctgctca accagcgcag
     7141 gactgtcgtg aacggagagc ataatcgtcg ccataccttt cggcagctgg cttagcagct
     7201 ccagaccgga gatatcgggc atggagatat cgcaaataca cacctgcaca ccgcgccccg
     7261 gcagccccgc cagcgcctcg cgccccgaac caaactcggc aactacctgc aaatcaggtt
     7321 ccagccccag cagctgcgca aagccggagc ggacgatgag gtgatcgtct ataagggcaa
     7381 cggtgatcat ggtcttgtcc tggcgggtaa aaaaaaacgc gcttacctta acgataagcg
     7441 cgatgttgtt caagccttga gcggttactg aaaaaacacc gcgatcttgt taaacatcgt
     7501 cggatcggac tgattacgct gcactttcac gacatcttcc agcttatcga tttggcttat
     7561 catctgctcc agacgctggt cgtcattgac cagtagccag atatggcttt tgtcgctgtc
     7621 ctgaatcggc agacaaagaa tgccttcaac gttaaaagcg cggcgggcaa aaaggccaca
     7681 aacgtgggtc attacgcccg gatggttgcg aacggtgagc tccagaatta cgttgtcatg
     7741 agttgtgttt tgcatggctt attcccccac catttcagta ttcgccgcac ctggcggcac
     7801 catcgggtaa actttttctt cggcatcaat gcgcacatgg atcagcgccg ggctagggcg
     7861 attgatgatt tcctgcaatg cagcctgcgg atcggcttcg ttattcaaat cacaggtttc
     7921 gaggccgaat ccggcggcaa tctgcataaa gttgattttg cccggatagg tggcggcaaa
     7981 aacgccttgc tcgtagaaca gactctgttg ctgatgcacc agccccagcg cttcgttgtt
     8041 catcagaatg attttgacat ccagctgatt ttcactggcg gtcgccatct cctgaatatt
     8101 catcatcagg ctgccgtcgc cggagaaaca caacactttg cgatccgggt tcgccagcgc
     8161 cgcgccaatc gccgcaggca ggccaaaacc catcgtgccc agcccaccgg aggtcagcca
     8221 ctggcgtggg cgattgagcg gataagcttg cgcggtccac atctgatgct gacccacatc
     8281 ggtggtgata atcgcattgt catcgacaca ggcggcaacg gcgttgatca ggccgtaatg
     8341 gcttaatgga tcgcacgctt tcgggattgg acacgggaat tcgcgctgca aatccgctac
     8401 caactggtgc cactctgcac gcggttgcgc ttccacctgc gggatcaatt gcgccagcac
     8461 gtcatcaaca tccgcctgaa tcgccacatg cggctgcttg attttaccca gctctgcgcg
     8521 atcgatatcg acatgaataa ttttggcatt cggacagaac tgctcggttt tgccaatcgc
     8581 ccggtcatca aaacgcgcac cgagcacaat caacaaatcc gcctcctgca aaatatagtt
     8641 ggtactgcgc acgccgtgca tccccagcat acccagcgac aacggatgcg cttttggcag
     8701 catgcccagc gccattaaag tcatggtggt aggcagttgc gctttctccg ccagttcacg
     8761 cacccgcgca ggcgcattga tcacaccacc acccagataa agcaccgggc gtttggcagc
     8821 gttaatcatt gtagctacgt cacgaatgct ttcttcgcta aaggcgggtg cagcggcttt
     8881 ttctgccacc gcgggctgag cttcaatctc aaaaaccgcc gtttgtacat ccttaggaat
     8941 gtctatccac accgggccag ggcggcctga ttgcgcaatg cggaaggcat cgctcatgac
     9001 ctgcgggagt tcttcgatat gtctgaccag atagttgtgt ttggtgatgg ggatagagat
     9061 gccgtaggtg tcgacttcct ggaaggcgtc ggtgccgatc atcgaggcgg gaacctgacc
     9121 agtgatgcaa atcagcggga tggagtccag ccgcgcatcg gcaatggcgg tcaccaggtt
     9181 agtcgcaccc ggtccgctac aggccataca gaccgccggt ttaccgtcgg tgcgcgccat
     9241 tccctgagcg ataaagcccg cgccctgttc atgacgggcc agaatatggc ggatttgcgt
     9301 gctttggctt aaggcatcgt aaacaggcag gatagaaccg cccggaatgc ctgtcacaat
     9361 cttaatgccc tgctgttcca ggaaatgaac gataaattct gcgccggtaa agcgcttacg
     9421 cgtcaatgtt gtgcccgaac ttgccatgct ccagtccttt tcttctgggc cgactttccg
     9481 ggaggtgctt aaacgaaaaa ccccgcccgg tttgcgccga cggggttttg gaatcgtgtg
     9541 ttgttccagt ccctacggcg cattgccgac gaccaccacc acacgcacga cgaccactgc
     9601 ggcggatggc gcagttggta gtagttttgc gttgaacatg gaagtagtca ttggggacct
     9661 tgttggtttt gtgtttaaca atatttatac aagcacagct ttacagggga gacaatggaa
     9721 aatttttcag caagggaaaa ttgaggggtt gatcacgttt tgtactgaat tgcagataac
     9781 aaaaaacccc gccggagcga ggtttcgtca gtcgcccgcg gctggtaacc gcaaagcaca
     9841 ctgtattatg tcaacacaga aagtatacgt gttccgcgca gaacgcgcaa tttcggcacg
     9901 aattttgacg tatttagtgc atagttgagt atcgatcaca gtttgcgttt tgtccaaata
     9961 ttactgttta tttatacagt aaacttctat aatatcactg tacgcaatgt gttatgcggg
    10021 ggccgcatcg ttacccggcg cactaagtcc tggctgaaac gggtggtgcc gtcagcgcct
    10081 taaccccgcg tgagcacact gtgttatgtc aacaagcaca aagtttctcc ttgagatacc
    10141 gcgtgcacaa cagctggcaa caggcagcgg aaaggtacgt cagctggcag tgctcctgaa
    10201 ccacaggaga cgcgtatgag cctggtggat atcgccattc ttatcctcaa actcattgtt
    10261 gcagcactgc aactgcttga tgctgttctg aaatacctga agtaattcag attcaagtcg
    10321 caccaaaggg gagcgggtaa ccgctcccct tttatattta gcgtgcgggt tggtgtcgga
    10381 tgcgatgctg acgtatctta tccgccctac catctctccc ggcaacattt attgccgctt
    10441 ttgtttacat attctgccgc taaacaactc cccattcctg gcgtatatct ggctaacatt
    10501 catcaatgtg atagattcct ctcccgcatt tatgggaatg cgtagtgact tactctaatt
    10561 atttttataa aagcatccgt gataatgaaa aggcatagaa acgtcaattt gttattgatg
    10621 ttggtattac tcgtggccgt cggtcagatg gcgcaaacca tttatattcc agctattgcc
    10681 gatatggcgc gcgatctcaa cgtccgtgaa ggggcggtgc agagcgtaat gggcgcttat
    10741 ctgctgactt acggtgtctc acagctgttt tatggcccga tttccgaccg tgtgggtcgc
    10801 cgaccggtga tcctcgtcgg aatgtccatt tttatgctgg caacgctggt cgcggtcacg
    10861 acctccagtt tgacggtatt gattgccgcc agcgcgatgc aggggatggg caccggcgtt
    10921 ggcggcgtaa tggcgcgtac tttgccgcga gatttatatg aacggacaca gttgcgccat
    10981 gctaacagcc tgttaaacat ggggattctc gtcagtccgt tgctcgcacc gctaatcggc
    11041 ggtctgctgg atacgatgtg gaactggcgc gcctgttatc tctttttgtt ggttctttgt
    11101 gctggtgtga ccttcagtat ggcccgctgg atgccggaaa cgcgtccggt cgatgcaccg
    11161 cgcacgcgcc tgcttaccag ttataaaacg cttttcggta acagcggttt taactgttat
    11221 ttgctgatgc tgattggcgg tctggccggg attgccgcct ttgaagcctg ctccggcgtg
    11281 ctgatgggcg cggtgttagg gctgagcagt atgacggtca gtattttgtt tattctgccg
    11341 attccggcag cgttttttgg cgcatggttt gccggacgtc ccaataaacg cttctccacg
    11401 ttaatgtggc agtcggttat ctgctgcctg ctggctggct tgctgatgtg gatccccgac
    11461 tggtttggcg tgatgaatgt ctggacgctg ctcgttcccg ccgcgctgtt ctttttcggt
    11521 gccgggatgc tgtttcctct ggcgaccagc ggcgcgatgg agccgttccc cttcctggcg
    11581 ggcacggctg gcgcgctggt cggcggtctg caaaacattg gttccggcgt gctggcgtcg
    11641 ctctctgcga tgttgccgca aaccggtcag ggcagcctgg ggttgttgat gaccttaatg
    11701 ggattgttga tcgtgctgtg ctggctaccg ctggcgacgc ggatgtcgca tcaggggcag
    11761 cccgtttaag cgcacgtcac cgcagcatcg tcatcagctc catgggagaa cgatgctgct
    11821 ttatcagatc acgcatcacc cgcatatgcg gtgcggagta agaataaaac gcctgatagc
    11881 ccgcacaaag cacgctgtct ggcgtctcct gccgagcgtg cgggcacagg cgatgccagt
    11941 cgcacgcctg gcagtcacag cgcggttgcg ggttttcgct gccacaccat ttttgtaagg
    12001 tggcgtcaaa aagctcaatc gagatacgac tgatatctgc ccgcacccaa cactcgaaca
    12061 gggcaattaa aaatttcccc cacagctctg ctgtcaccga agcggccgtc agatcgcccc
    12121 ggtcatcaaa ttcaagatag ggaatgagct gtacggagcg gatgccccgc tgtttgaaat
    12181 tgtgataaaa atcggcaggt tgcagagcat ccttgtactc tacaaccagt ttatgcctgt
    12241 cctcttcagc atcaatgacc tgacttcctg tcatgcgact ctctcaataa atacgataag
    12301 ttgatgtata tgcgttacag caaacagtat tgcgagagat aacaccgcga gggagatcaa
    12361 aaatttgtca cggactacgt gaaattggga aaaatcgcta ttcgtgacat ccattaaatt
    12421 gcgattacgg gtgtagtgcc agaggatcag cgccacgatg gcgagaatgc caatcgaaat
    12481 ccagaataac atgcccgcct ggtgccagtt gtgtttgata gccaacgcca tcaacgcgcc
    12541 gtagcccagc atggtacgaa accacgccag tgatgtgcgc tccggctgca gtcctgggtc
    12601 ggcaatgcgt ctggctttgc ggctatccgg catacaacac cagtcccata acgatcaccg
    12661 ccacgaccat caatattaag ctaatgatta ataagctgtt ggtgtagggc aggtcctctt
    12721 tcagacgcat cgctttttca ttgcgtaacc agcgcaggta gccatacatt gctaacccgc
    12781 cggaaaacag acacaacaac aacgccagca gttcacgaat gacgggcgtg gcgaaatctg
    12841 gcgcaagttg atcaagccca acccccgccg ccagaaatcc tagtgcggta cggatccacg
    12901 ccagaaaagt acgctcatta gccagcgaga agcggtaatc gggcgcttct ccaaggcggg
    12961 aaattttcat tgttgctcct ttgcaatttc actaactctc tacctttagc ggtttttttt
    13021 aagaatttcg tccgcccgtt tcctgatatg cttttgccat ccgctacaag gaggtcagat
    13081 gggtatcata gcgcaaaata aaatcagttc attgggcatg ctgttcggtg ccatcgcttt
    13141 aatgatgggg attatccact tttcattcgg ccctttttcc gcaccaccgc caacgcttga
    13201 aagcatcgtg gcggacaaaa cggctgaaat taaaagaggt cttctcgccg gaataaaagg
    13261 cgaaaaaatt accaccgtgg agaaaaaaga ggacgtagat gtcgataaaa tcctcgatca
    13321 aagcggtatc gcactggcca ttgctgcgct actttgcgca tttgttggcg gtatgcgtaa
    13381 ggaaaatcgc tggggcattc gcggcgcgct ggtttttggc ggcggaacgc tggctttcca
    13441 taccttgctt tttggtatcg gcatcgtgtg cagcattttg ctgatattcc ttatcttttc
    13501 cttcctgacc ggcggttcgc tggtttaagc agcctgaata atgcccgccg ggtgatcatc
    13561 agcgggcatt atcgtcagaa cttctgtttt ttctcttcca ccttcacccc ttgtgtcggt
    13621 aggccggtgt catagtcacg taccaccggc gaatagccat cttgtgggcg tggacgaaat
    13681 gcccccatcc agcgcggccg tgcatcttta cgccacggac gcaggctcca ttgttgacat
    13741 cctccacgcc ctgaaggacg tggatccctg ctacgttcag gctgtcgcct gaatcatttc
    13801 ggtgggttcc tgcttcaacg agcggcctga ctgcaccatc tctccacagg caagaacggc
    13861 gtgccccgcc gctaaaatgt tacgagcgcc atttacatcg gcgttcgctg tatatccaca
    13921 tacctggcat ctgaatttac tttgtgacag gcgattttct ttcgctgtat gaccacagca
    13981 cacgcaacgc tggcttgtgt acgctggcgg aacagcaagc acctgaccac cacgccagag
    14041 ctgcttgtac gcaagctggc ggcgcatttc ataccagccc tgatccagta tcgaacggtt
    14101 taaacctgat tttgcccgga cattgcgccc cggcaggctg accgtacccg ctgctgactt
    14161 tgacatgttg ctgaccttca aatcctcaat gactatcatt gcgtggtttt tgctgacggt
    14221 cgttgtgact ttgtgaaggt agtccctgcg gatatttgcg atacaggaat gcagtcgctg
    14281 tattttgcgt ttctgcttct gccagttgtt gctgaatttg accttgcggc ttaactgtcg
    14341 ctgaagtctc gccagcttct tctggttttt ctgaaaactg tttacaggct caaagactgt
    14401 gccatctgac agcgtggcga gtttagccac gccagcatcc agcccgacca ttgatgctga
    14461 agggtgaact ggagttgata cttcactttc tgtctgaata ctgatgtacc acttaccgca
    14521 ggactggctg acagtgacat ttttcacaac acccgtgacc tgacggctgt tccggtagcg
    14581 catccagccc agtttcggca gaaaaatacg gctgttttcc tgatcgagct taacgccctg
    14641 cgggtagcgg aatacatcat tctgtccccg ctttttgaat cggggaaaag cagcccgatt
    14701 ctggaagaag tttttgtaag cccgctcaag gtctttcagt gactgttgca atggctgtga
    14761 cggggcatct ttaagccatt gcgtttc
//
LOCUS       DAKISA010000103        14762 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.103, whole genome shotgun sequence.
ACCESSION   DAKISA010000103 DAKISA010000000
VERSION     DAKISA010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14762
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.103"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            289..954
                     /gene="mcbR"
                     /locus_tag="OVW45_003357"
     CDS             289..954
                     /gene="mcbR"
                     /locus_tag="OVW45_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCV5527726.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLTELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(990..3092)
                     /locus_tag="OVW45_003358"
     CDS             complement(990..3092)
                     /locus_tag="OVW45_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCV5527727.1"
                     /translation="MKIFSVRQTVLPALLALSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTDVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLLA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            3334..4395
                     /locus_tag="OVW45_003359"
     CDS             3334..4395
                     /locus_tag="OVW45_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HCV5527728.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGVTINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(4510..6009)
                     /gene="ansP"
                     /locus_tag="OVW45_003360"
     CDS             complement(4510..6009)
                     /gene="ansP"
                     /locus_tag="OVW45_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HCV5527729.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            6276..6893
                     /locus_tag="OVW45_003361"
     CDS             6276..6893
                     /locus_tag="OVW45_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HCV5527730.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGSGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
     gene            6969..7181
                     /locus_tag="OVW45_003362"
     CDS             6969..7181
                     /locus_tag="OVW45_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncH family protein"
                     /protein_id="HCV5527731.1"
                     /translation="MLCFLIYITLPFSQLVYFISSEKKLTIHIVQMFHLLSQVFYNLK
                     KFLMMDMLGVGDAININANKNIRQVC"
     gene            8002..10110
                     /locus_tag="OVW45_003363"
     CDS             8002..10110
                     /locus_tag="OVW45_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCV5527732.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWPGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASDLHGEQPQAVPGR
                     RGSGTTLENHFAVIPADRTWRPQPLMKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAETIGNNQMIAVTNNQIETVGVNQIETVGSNQIIKVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDD
                     TGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAA
                     SKSPPKTPDEKQDTPDMREY"
     gene            10178..13930
                     /gene="rhsD"
                     /locus_tag="OVW45_003364"
                     /pseudo
     CDS             10178..13930
                     /gene="rhsD"
                     /locus_tag="OVW45_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsD"
BASE COUNT         3806 a         3636 c         4024 g         3296 t
ORIGIN      
        1 cggtttagag aatgcgccac agacgtttat cggcctgctg aagggtaaaa acttcggcaa
       61 agtggtgatc cgcgtcgcgg gtgatgatta aagtttcaac ggcggcgtaa gccgccataa
      121 aaatccaggc aaaaagctta ttttcagctt taattaacta accacgtcat tgttctgctg
      181 ctaataacaa caaaggtggg tggtttatga ttattcactt taatacacca ggtgaactcc
      241 ttctgccgtg caggcagggt tggacagaaa acacgcgaag gaattaaaat gcctggaacg
      301 ggaaaaatga aacatgtcag tttgactctg caggttgaga acgacctgaa acatcagctt
      361 agtattggcg cattaaaacc tggcgcacgc ctgattacta aaaatctggc ggagcaatta
      421 ggtatgagta ttacacctgt gcgtgaagca ttattacgtc tggtttcggt gaatgcgctt
      481 tctgtcgcac ctgcacaagc atttacagtt ccggaagtgg ggaaacgtca attagacgaa
      541 atcaatcgga tccgctacga actggaatta atggcagttg ctctggctgt tgaaaacctc
      601 accccgcaag acctgacgga actccaggaa ttgctcgaga aattacagca ggcgcaagaa
      661 aagggcgaca tggaacaaat cattaatgta aacaggctat ttcgcttagc gatttatcat
      721 cgctcaaata tgcccatcct gtgtgagatg attgagcaac tgtgggtcag gatggggcct
      781 ggtttacatt atctctatga agcgattaat ccagcggaat tacgggagca tatagaaaac
      841 tatcatctgt tactcgctgc gttaaaagca aaagacaaag agggatgcag acattgtctt
      901 gctgaaatta tgcaacaaaa tattgctatt ttataccagc aatacaatcg ttaaagtaaa
      961 tgcccggcaa ataccgggca tcaaggtaat tactcaaatc tccacgcaat attcatgccg
     1021 acaccataat ttcgtccggg cgaaggttcg taatatcgcc cgtttgactc attgacaatg
     1081 acagaaccaa cgtattcttt atcgaataaa ttatcgacac gaccaaataa atccacagtt
     1141 aaattgtggt aattgtattt atacccggtg aataagccga cgagagtata agacggcgct
     1201 tttgccgtat tttcatcatc tgccataata tcgcccatat aacgcgcttc cgtgcctgca
     1261 taccaaccat cttccggtac ataacctatc gacgcaaagc ccatattacg ggcgatccct
     1321 ggcatccgat taccgttaca atcctgttca ttgcaaacat tgctgcgata ggtcgcatca
     1381 agccaggtcc aggacgcgtt tacgcgaaaa tctcctgcga aacgttgatc ccatgccagt
     1441 tcagcgcctt gacgacgggt ctttccggca tttttgtaag tcgtacgccc accgctactg
     1501 ctatcgacaa caatttcatc atcagtgtcg gtctgaaaca atgcgagact aagcagccca
     1561 tcaccaatac gcgttttact gccgatctca attgtatcgt tggtggatgg ttttaaacca
     1621 aagttcatac cgctttgccc atcagcacga taagacagct cattaatcgt cggcgtttca
     1681 aaacctcgcc cggctgccag atagatattc caggcatcgg tcattgcata ttttaacgaa
     1741 ccggcaagta gccatttatg ataactggca tcaccgctgt catcgccgtt acccggagta
     1801 acgtaatggt cgttggaatc aaaccacacg gagctgtagc gcacgccagc atccagcgac
     1861 agtttttcgc tcagctgcca ctgcgtctgt aaatagggat cgatgttcca catcagattg
     1921 cgttcgtcgc gacgcaactc acctttttgc ccgtactccg gcatgccgct attcaggcgg
     1981 aagttattgt agcccttgcg gttttcactc atgttttcgt agttcaggcc ggtagtgaac
     2041 gtgaccggaa cgcccagttc accacggtgt gtccagcggc tgtctatccc ctggtaatgg
     2101 cgttgcaggg taatcacgcc gcccgcatgt gacgggttaa gttgtggtgc catgggtatt
     2161 gactggtact gggtcgtttc tcgctctccg gcatacatca tcacactcat atcatcttgc
     2221 gcgctcaggc tacgctcata gcgcaaccca gcctgagttt gcttgatggt ttttcgcgtg
     2281 tcgtactgtt ctgcacgagg cgcttgttgt ggattagcct tccattctgc tttggttagc
     2341 ccacccgggt catctgcttt gatatccaca ctattgaaaa tcagacttaa tttgctggct
     2401 tcatcaatgc gtacgcccag tttggcattg gctaaatttt tctgtgcgcc actatggtca
     2461 cgatagccgt gggtcgtaaa acgcgtggtt gagacggtgt aatcgacatc gccaggctgt
     2521 gtgccgtctc ccgttgcgcc cgttgctttc agcccatagc gccagctgcc aaaactgccg
     2581 tagtaactac tggcttcaat ggttggtggc tgttgtccgg tctgggtggt gacattcatt
     2641 accccaccag acgcgttgcc atacagggca gagaaggggc cacgcagcac ttccacattt
     2701 tgcacactgc ttaaatcgat gttggatgtt tgcccttgcc cgtcgggcat ggtggcggga
     2761 ataccgtcca catacaggcg aataccgcga ataccgtaag tggagcggga gccaaatccg
     2821 cgaatcgaca gctgtaaatc ttgcgcatag ttctgccggt tttgtacctg caaaccaggc
     2881 acgtcggtca gtgattcgga caagttaatg cgcggtgttg ccaggcgcat ctcctcgcca
     2941 tccaccacgc ttactgctgc tggggtatcc agttctgaaa ccacctgcgg tgcggcactg
     3001 acaatcatgg tctgttcatc agcggcaaaa acaacggggg aaagggcaag tagtgcgggc
     3061 aaaacggtct gtcggacgga aaaaatcttc atgaaaaaag ccaggttaag aatgggaaaa
     3121 cgccgttatg gtaataaaat tgtaaattca tggaaaacga aacggcacga tacgttaagt
     3181 aattgagaaa attgtagtcg taacggcaag aaatgctcca catttgagaa aataatgatt
     3241 accattccca tttataacaa gagcgtaacg atgattacgc ttagcgaagc attgtgaagc
     3301 agcaaaaata tcggttcatc aaagggagtc gtcatgcatt tacgtcatct gttttcatcg
     3361 cgcctgcgtg gttcattact gttaggttca ttgcttgttg cttcatcatt cagtacgcag
     3421 gccgcagaag aaatgctgcg taaagcggta ggtaaaggtg cctacgaaat ggcttatagc
     3481 cagcaagaaa acgcgctgtg gctcgccact tcgcaaagcc gcaaactgga taaaggcggc
     3541 gtggtttatc gtcttgatcc ggtcactctg gaagtgacgc aggcgatcca taacgatctc
     3601 aagccgtttg gtgtcaccat caataacacg actcagacac tgtggtttgg taacactgta
     3661 aacagcgcgg tcacggcgat agatgccaaa acgggcgagg tgaaaggccg tctggtgctg
     3721 gatgatcgta agcgtacgga agaggtgcgc ccgctgcaac cgcgtgagct ggtagctgac
     3781 gatgccacga acaccgttta catcagtggt attggtaaag agagcgtgat ttgggtcgtt
     3841 gatggcgaga atatcaaact gaaaaccgcc atccagaaca ccggtaaaat gagtaccggt
     3901 ctggcgcttg atagcaaagg caaacgtctt tacaccacta acgctgacgg cgaattgatt
     3961 accatcgaca ccgccgacaa taaaatcctc agccgtaaaa agctgctgga tgacggcaaa
     4021 gagcacttct ttatcaacat tagccttgat accgccaggc agcgtgcatt tatcaccgat
     4081 tctaaagccg cagaagtgtt agtggtcgat acccgtaatg gcaatattct ggcgaaggtt
     4141 gcagcaccgg aatcactggc tgtgctgttt aacccagcgc gtaatgaagc ctacgtaacg
     4201 catcgtcagg caggtaaagt cagtgtgatt gacgcgaaaa gctataaagt agtgaaaacg
     4261 ttcgatacgc cgactcatcc aaacagcctg gcgctgtctg ccgatggcaa aacgctgtat
     4321 gtcagtgtga aacaaaaatc cactaaacag caggaagcta cccagccaga cgatgtgatt
     4381 cgtattgcgc tgtaatagat atggcgaggg tgaaaacgga gagacttgcc tgacttttat
     4441 cacgccgcat gcggcacacc ttcgtcagta atctaaattt gcccggcacg tagccgggca
     4501 attttgcaat tagctcgccg tttcaggctt aaacacaatt tcctgttttt cttcatcttc
     4561 ctcgacgact ggcgcagtgc tgtgaatttc agcaacgcgt ttgcgcacac caaaccagcc
     4621 tataaccagc agaataccga taatcggcag cgccgcgata gtataagtcc cgttcgggta
     4681 atcgaacgcc atcagcacaa ggacactcag taaaaacagt aatgtcagcc aggaagtgaa
     4741 gggcgcgcca ggcagtttaa aactgacatc cgctgctttg ccttctttaa tcgctttacg
     4801 caggcgcatc tggcacacga tgataaacgc ccatgaagcg atgattccca gcgacgcgaa
     4861 gttcaacaca atctcaaata cgcgcgacgg caccagatag ttgaggaata cgccgacgac
     4921 atacacaacc agtgtcgcca gaatcccggc atacggcaca tgctgacgac tcattttcgc
     4981 cataaaactc ggtgcggaac cgcccatcgc catcgagcgc agaatacgcc cggtgcagta
     5041 cagacctgag ttcaggctgg agagggcagc ggtcagcacc acaatgttca taatgctgcc
     5101 gatatatggc acacccagtt tagagaaaaa cgtcacgaac ggactttgcc ccgcctgata
     5161 cgcgctccac ggcaataaca taaccagcaa caccacggag ccgacgtaaa acaggccaat
     5221 acgccaaatc acactgttaa tggctttagg caccatggtc tgcggatctt tacattctcc
     5281 ggcagctgta cccaccattt caatggaggc aaaagcaaac actacgccct gaatcaacac
     5341 cagcgcaggc agcaaaccgt gggggaagaa gccgccatta tcggtgatta aatgaaagcc
     5401 agtggtgttg ccatccagcg gctgaccact accgaggaac actgtaccca cgaccaaaaa
     5461 ggtcacaatg gcgagcactt taataagcgc aaaccagaac tccatctccg caaaccattt
     5521 cacaccgatc atattcatgg tgccaacgat ggtaagtgca gcgagcgcaa agacccactg
     5581 cggcacgccg ccaaacgcac cccagtaatg catatacaga gcgacggcgg taatatcaac
     5641 aatccccgtc atcgcccagt tgatgaagta catccagcca gcaacataag cggctttctc
     5701 acccaaaaac tcacgggcat aagagacaaa actgccactg gaagggcggt gtagcaccag
     5761 ctcacccaat gcacgcagaa taaaaaacga aaacaagcca caaattaaat aaaccagtgc
     5821 cagtgctgga cccgccattt gcagtcgggc tcctgcacct aaaaacaagc cggtgccaat
     5881 cgcgccgcca atggcgatca tctgtacctg gcgattgccc atcgctttgt gatacccctc
     5941 ttcgtgggca ttaagccagc ggcgtttcgc ggcgtgttga tctgaagtgt cggtgtcgtg
     6001 tttactcatt gctctccctg attgctttaa tgaaaaagtc atataagttg ccatgaacaa
     6061 tgtttattca tcctggcgat gatagttatc accattattc tttatgggta taaaactatc
     6121 ggcgcagcat cctacccgtt atcgatatac gatgcaaaac atccccttac aatcctgaag
     6181 gggattcata caactgacga aaaaatgaca aatacttttg ctggttaacc tgtgtactgt
     6241 cctacactta atctttaaaa gattgtgagg ggcatatgat taaggtgtat ggcgtacccg
     6301 gatggggctc tacaatcagt gagctgatgc taacactggc tgatattcct tatcagttcg
     6361 ttgatgtcag tggttttgac cacgagggaa cttcacgcga gttattgaaa accctgaacc
     6421 cgttgtgtca gattccgaca ctggcgctgg aaaatgacga gattatgacg gagacagcgg
     6481 cgattgcatt gatggttctc gatcgtcgcc cggaccttgc gccaccggtt gggcgcgccg
     6541 aacgtcagca gtttcaacgg ctattggtct ggctggttgc taatgtctat ccaacattca
     6601 ctttcgccga ttaccctgag cgttgggctc ctgacgcacc tgaacagtta aagaagaatg
     6661 ttattgaata tcgtaaatcg ctttatatct ggctgaattc acaattgacc gctgaacctt
     6721 atgcgtttgg tgagcaacta acgctggtgg actgttatct ttgcactatg cgcacatggg
     6781 ggtctgggca tgaatggttt caggataatg ccacgaatat cagtgcgatt gctgatgctg
     6841 tatgtcagtt accgaaatta caagaagtat taaaaaggaa tgaaattatt tgagtgggat
     6901 accagaggat tatgttattt attattcagt aactttatcc acaatgatgg gcgtaattaa
     6961 ttaaattcat gttatgtttt ttgatttata tcactctccc cttcagtcag cttgtctatt
     7021 tcatttcctc tgaaaagaaa ctaactattc acattgtgca gatgtttcac ttgttatcac
     7081 aggtattcta taatctcaaa aagtttttaa tgatggatat gctcggagtt ggagatgcaa
     7141 ttaatattaa tgcaaataaa aatatccggc aggtatgcta aaaggataaa tggcgttctc
     7201 tgtatttatt cgcaattatc tataggacaa atatggagag cttgatgagt cagagctaaa
     7261 acagcaattg ataaataaat atgttgattt attcaagaaa atggatgtat tttaaatatt
     7321 gattcttaat ataacctaat caatagatta tcacgtgaga gctgcacatt attggcgggg
     7381 ccggggaaaa catcattttg ggaacaatga atcaggtact cttaccactg aagcaagatc
     7441 atccgggcac caggtggtgg tgcagtgctt tggaaatggt cctctcgggg ggagttgcca
     7501 ttattttcta tgctatttgc ttattatatt catcaggtga atttaataaa ttcctaattt
     7561 gaaatatcca ctattaagcc agtgtttaac gacgttaaga tggcaatgtg cagatcatgt
     7621 tatcagggtg ggcggtagcg tatattcctt atacaaacca tcattcattt caatgattgt
     7681 tattgaatta tttattgttg tgaataaaaa gacattatga atattggttt gactttgcat
     7741 atcaggtttt ttattactcg ctgtaatgta cgagcctgtc gtgaataacg tcgagaaccc
     7801 atccgttgcg tttgtgatta ttttgttgac taaacagaca cccgtttctc tgaagtaaaa
     7861 tcccagacta aatcatcaca taaccatgac atttttctga tattccccgg taacgccaga
     7921 tgtcgactcg cttatctcat ccgcccgaca tgttgtacat gcaatatgtg cggattatta
     7981 ttcattttca cggaggttgc tatgtcaacc ggattacgtt tcacactgga agtggacggc
     8041 ctgccaccgg atgcttttgc ggtggtttcc tttcatttga accagtcact ctcttcgctc
     8101 ttttccctcg atctctctct ggtcagccag cagtttctct cccttgaatt tgcgcaggtg
     8161 ctggacaaaa tggcctacct gacggtatgg cagggcgatg acgtacagcg ccgggtgaaa
     8221 ggtgtggtga cctggtttga actgggggag aacgacaaaa accagatgct gtacagcatg
     8281 aaggtatgcc caccgctgtg gcgcacaggg ctgcgccaga acttccgtat cttccagaat
     8341 gaggacatcg aaagcatcct cgcgacgatc ctgaaggaaa acggtgtgac cgagtggagt
     8401 ccgctgttca gcgagccgca tccttcccgt gagttttgtg tccagtacgg cgaaactgat
     8461 tacgatttcc tgtgccggat ggcggcggag gaaggcatct tcttttatga ggagcacgcg
     8521 caaaaaagta ccgaccagag cctggtcctg tgcgacaccg tgcgttatct gccggagtcc
     8581 tttgagatcc cctggaaccc gaacacccgt accgaggtaa gtaccctctg catcagccag
     8641 tttcgctaca gcgcacaaat ccgtccttct tccgtagtga ccaaagacta cacctttaaa
     8701 cgtcccggct ggccagggcg ttttgatcag gaaggccagt accaggatta ccagcgcacg
     8761 cagtatgaag tgtatgacta ccccggacgt ttcaagggtg cccacgggca gaactttgcc
     8821 cgctggcaga tggacggctg gcgaaacaac gcagaagtgg cgcgcggaac aagccgttcg
     8881 ccggagatat ggccgggacg gcgaattgtg ctgacggggc atccgcaggc gaacctgaac
     8941 cgggaatggc aggtggtggc gagcgatctt cacggcgaac agccgcaggc ggtaccggga
     9001 cgcaggggtt caggcaccac gctggagaac cattttgcgg taatcccggc agacagaaca
     9061 tggcggccac agccgttgat gaaaccgctg gtggatggcc cgcagagcgc cgtcgtgacg
     9121 ggaccggcag gcgaggaaat cttctgtgat gaacatggcc gcgtgcgggt gaaatttaac
     9181 tgggaccgct ataacccgtc aaaccaggac agttcatgct ggatccgtgt ggcacaggcg
     9241 tgggcaggca ccggctttgg caacctggcg ataccgcgtg tgggtcagga ggtgattgtg
     9301 gacttcctca acggcgaccc ggaccagccg attattatgg ggcgtaccta ccaccaggaa
     9361 aaccgcacac ccggcagcct gccggggacg aagacgcaga tgaccattcg ttcgaaaacc
     9421 tataagggca gcgggtttaa tgaactgaag tttgacgatg cgacagggaa agaacaggtc
     9481 tacatccacg cgcagaagaa catgaacacc gaggtgctga ataaccgcac cacggatgtg
     9541 ataaacaacc atgctgaaac cattggcaac aatcagatga ttgcggttac caacaatcag
     9601 atagagacgg tgggcgttaa ccagatagag acggtgggca gtaaccagat aattaaagtg
     9661 ggttctgttc aggtggaaac gattggactt gttcgtgcgc tgacggtggg cgtggcgtat
     9721 cagacgacgg taggtggcat tatgaacacc tcggtggcac tgatgcagtc ctcgcagatc
     9781 ggtttgcata aatcgttgag ggtcgggctg ggttatgacg tcaaagtcgg aaataacgtt
     9841 accttcaccg ttggtaaaac gaaaaaggat gataccgggc agaccgcgat ttactccgcc
     9901 ggtgagcatc tggagctctg ctgtggtaag gcaaggctgg tgctgacgaa ggacggacaa
     9961 atttttctca acggcacaaa aattcatttg cagggtaagg agcaggttaa tggtgactca
    10021 ctgttgatta actggaactg tgcagcctcg aaatccccac cgaagacccc tgatgaaaag
    10081 caggatacgc cggatatgag agagtactga tcccgtacct gttcggtatt cagtaacaaa
    10141 ccagcattaa aaataaacag aagaaggaat ttgaatcatg agcggaaaac cagcggcgcg
    10201 tcagggagat atgactcagt atggcggtcc cattgtccag ggttcggcag gtgtaagaat
    10261 tggcgcgccc accggcgtgg cgtgctcggt gtgtccgggc gggatgactt cgggcaaccc
    10321 ggtaaatccg ctgctggggg cgaaggtgct gcccggcgag acggaccttg cgctgcccgg
    10381 cccgctgccg ttcattctct cccgcaccta cagcagctac cggacgaaga cgcctgcacc
    10441 ggtgggcgtt ttcggccccg gctggaaagc gccttctgat atccgcttac agctacgtga
    10501 tgacggactg atactcaacg acaacggcgg gcggagcatt cactttgagc cgctgctgcc
    10561 gggggaggcg gtgtacagcc gcagtgagtc aatgtggctg gtgcgcggtg gtaaggcagc
    10621 acagccggac ggccatacgc tggcgcggct gtggggggcg ctgcgccgga tatccggtta
    10681 agcccgcatc tttacctggc gaccaacagc gcacaggggc cgtggtggat actggggggg
    10741 tctgagcggg tgccgggtgc tgaggacgta ctgccagcgc cgctgccgcc gtaccgggtg
    10801 cttaccggga tggcggacgc tgacgtaccg gcgtgaggcc gccggtgacc tggccgggga
    10861 aatcaccgac gtgacggacg gtgccgggcg ggagttccgt ctggtgctga ccacgcaggc
    10921 gcagcgtgcg gaagaggccc gcacctcttc gctatcttct tctgacagtt cccgccctct
    10981 ctcagcctca gcgttccccg acacactgcc cggtaccgaa tacggccccg acaggggtat
    11041 ccgcctttcg gcggtgtggt tgatgcacga cccggcatac ccggagagcc tgcccgctgc
    11101 gccactggtg cggtacacgt atacggaagc cggtgaactg ctggcggtat atgaccgcag
    11161 caatacgcag gtgcgcgctt tcacgtatga cgcgcagcac ccgggccgga tggtggcgca
    11221 ccgttacgcg ggaaggccgg agatgcgcta ccgctacgac gatacggggc gggtggtgga
    11281 gcaactgaac ccggcagggt taagctaccg ctatctttat gagcaggacc gcatcaccgt
    11341 caccgacagc ctgaaccggc gtgaggtgct gcatacagaa ggcggggccg ggctgaaacg
    11401 ggtggtgaaa aaagaactgg cggacggcag cgtcacgcgc agcgggtatg acgcggcagg
    11461 aaggctcacg gcgcagacgg acgcggcggg acggcggaca gagtacggtc tgaatgtggt
    11521 gtccggcgat atcacggaca tcaccacacc ggacgggcgg gagacgaaat tttactataa
    11581 cgacgggaac cagctgacgg cggtggtgta cccggacggg ctggagagcc gccgggaata
    11641 tgatgaaccg ggcaggctgg tatcggagac atcgcgcagc ggggagacag tacgctaccg
    11701 ctacgatgac gcgcacagtg agttaccggc gacgacaacg gatgcgacgg gcagcacccg
    11761 gcagatgacg tggagccgct acgggcagtt gctggcgttc actgactgct cgggctacca
    11821 gacccgctat gaatacgacc gcttcggcca gatgacggcg gttcaccgcg aggaaggtat
    11881 cagcctttac cgccactatg acaaccgtgg ccggttaacc tcggtgaaag acgcacaggg
    11941 ccgtgaaacg cagtatgagt acaacgccgc aggcgacctg actgccgtta tcaccccgga
    12001 cggcaaccgg agcgagacac agtacgatgc gtggggaaag gcggtcagca ccacgcaggg
    12061 cgggctgacg cgcagtatgg agtacgatgc tgccggacgt gtcatcagtc tgactaacga
    12121 gaacggcagt cacagcgact tcagctacga tgcgctggac cggctggtac agcagggcgg
    12181 ctttgacggg cggacgcaac gttatcatta tgacctgacc ggaaaactca cgcagagtga
    12241 ggatgagggg cttgtcaccc tctggcacta cgatgcgtcg gaccgcatca cgcaccggac
    12301 ggtgaacggc gacccggcag agcagtggca gtatgacggc cacggctggc tgagagaaat
    12361 cagccacctg agcgaaggcc accgtgtcgc tgtccactac ggctacgacg ataagggccg
    12421 cctgacgggc gaacggcaga cggtggagaa cccggagacg ggggaactgc tgtggcagca
    12481 tgagacgaaa cacgcctaca acgaacaggg gctggcaaac cgcgtcacgc cggacagcct
    12541 gccgccggtg gagtggctga cctacggcag cggttacctg gcgggcatga agctgggcgg
    12601 gacgccgctg gtcgagtata cgcgggacag gctgcaccgt gagacggtgc gcagcttcgg
    12661 cagcagggca ggcagtaatg ccgcatatga actgaccagc acatacaccc ccgcaggcca
    12721 gttacagagc cagcacctga acagcctggt gtatgaccgt gactacgggt ggaatgacaa
    12781 cggcgacctg gtgcgcatca gcggcccgcg acagacgcgg gaatacggct acagtgccac
    12841 gggcaggctg gagagcgtgc gcaccctcgc accggacctg gacatccgca tcccgtatgc
    12901 caccgacccg gcgggaaacc gcctgccgga cccggagcta cacccggaca gcacgctcac
    12961 ggcgtggccg gataaccgca tcgcagagga tgcgcactat gtttaccact atgatgaata
    13021 cggcagactg acggagaaga cggaccgcat cccgacgggt gtgatacgga cggacgacga
    13081 gcggacccac cactacgaca gccagcaccg cctggtgttc cacacgcgga tacagcatgg
    13141 cgagccactg gtcgagagcc gctacctcta cgacccgctg ggacggcgaa tggcgaaacg
    13201 ggtatggcgg cgggagcgtg acctgacggg gtggatgtcg ctgtcgcgta aaccggagga
    13261 gacatggtac ggctgggacg gcgacaggct gacgacggta cagaccgaca ccacacgtat
    13321 ccagacggta tacgagccgg gaagcttcac gccgctcatc cgggtcgaga cagagaacgg
    13381 cgagcgggag aaagcgcagc gccgcagcct ggcggagaag ctgcagcagg aagggagcga
    13441 ggacgggcac ggcgtggtat ttccggctga actggtgcgg ctgctggaca gactggagga
    13501 agaaatccgg gcagaccgcg tgagcagtga aagccgggtg tggcttgcgc agtgcgggct
    13561 gacggtggag caactggcca gacaggtgga gccggaatac acaccagtgc gaaacgttca
    13621 tctttaccac tgcgaccatc ggggccagcc gctggcgctc atcagcgaag acggcgatac
    13681 ggtgtggagc ggggcgtatg atgagtgggg caaccagcta aatgaggaga acccgcatca
    13741 tgtgtatcag ccataccgtc tgccggggca gcagtatgat gaggagtcgg ggctgtacta
    13801 caatcgtcac cggtactatg atccgttgca gagacggtac atcacccagg accccattgg
    13861 gctgaaaggt gggtggaatc tgtatcagta tccgctgaat ccggtgagca ggatagatcc
    13921 gttggggtta ttctcatgtg agagttgtag tcattttata gatgatataa ataataaact
    13981 ggataacctt ccgtttaacc cgacaaaaat gtttacagct agtttaaata ctgctaattc
    14041 agcaaggctg tatgcaagtg gcatgacaaa attcggtata agtgttgctg ccgatggaac
    14101 gatcattgct gccccagtcg gtgcagcttc tatggcttta ggggcgtgga atataaaatc
    14161 tgcgacagct gcatataaca gagcaaattt acaattatca gaatcacttc aggaaaatgg
    14221 tagagatagg tgttggagaa atttattggg tattttacct tatggtgagc agtttgatga
    14281 ccctggtgaa ccatcaatat tagacgttta tggtgaaaag attgagaata tcaaagattc
    14341 tccaatgaag ttttttgaag agcttgggac gctggggttg taatgtgtat ttttaatgat
    14401 atttttatgt ttgtgtttat tgttagtagt atacttgtaa ccgtgcattt tgtgtattct
    14461 ttatttttta aaagtggaaa ggtaaaagat aaggtaaaaa aatggattaa agtaatgtgt
    14521 gatttatttt gggggatagg atagtggtgt gatatggata aatataaatt acagtggctg
    14581 gcatatttcg acctgacgcc ggagtagata aaaaaacaga caggattgga gtatagcaag
    14641 cgtctgacaa tctctcggga cagtagtatg atgaagaggc ggggttgtac tataaccgta
    14701 accggtacta cgatccgcta aagggcaagt atatgactca gggaaagatc atcagaagtt
    14761 ca
//
LOCUS       DAKISA010000104        14367 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.104, whole genome shotgun sequence.
ACCESSION   DAKISA010000104 DAKISA010000000
VERSION     DAKISA010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14367
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.104"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..694)
                     /locus_tag="OVW45_003365"
     CDS             complement(<1..694)
                     /locus_tag="OVW45_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCV5527733.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRA"
     gene            950..1105
                     /gene="rseD"
                     /locus_tag="OVW45_003366"
     CDS             950..1105
                     /gene="rseD"
                     /locus_tag="OVW45_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCV5527734.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            1102..1677
                     /gene="rpoE"
                     /locus_tag="OVW45_003367"
     CDS             1102..1677
                     /gene="rpoE"
                     /locus_tag="OVW45_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCV5527735.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            1710..2360
                     /gene="rseA"
                     /locus_tag="OVW45_003368"
     CDS             1710..2360
                     /gene="rseA"
                     /locus_tag="OVW45_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCV5527736.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWHKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGLQTLGTQSQ"
     gene            2360..3316
                     /gene="rseB"
                     /locus_tag="OVW45_003369"
     CDS             2360..3316
                     /gene="rseB"
                     /locus_tag="OVW45_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCV5527737.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVISFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            3313..3792
                     /gene="rseC"
                     /locus_tag="OVW45_003370"
     CDS             3313..3792
                     /gene="rseC"
                     /locus_tag="OVW45_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCV5527738.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPDLVRFETSSEDASQ"
     gene            3990..5789
                     /gene="lepA"
                     /locus_tag="OVW45_003371"
     CDS             3990..5789
                     /gene="lepA"
                     /locus_tag="OVW45_003371"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCV5527739.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            5805..6779
                     /gene="lepB"
                     /locus_tag="OVW45_003372"
     CDS             5805..6779
                     /gene="lepB"
                     /locus_tag="OVW45_003372"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCV5527740.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            7052..7732
                     /gene="rnc"
                     /locus_tag="OVW45_003373"
     CDS             7052..7732
                     /gene="rnc"
                     /locus_tag="OVW45_003373"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCV5527741.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            7729..8634
                     /gene="era"
                     /locus_tag="OVW45_003374"
     CDS             7729..8634
                     /gene="era"
                     /locus_tag="OVW45_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCV5527742.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            8646..9374
                     /gene="recO"
                     /locus_tag="OVW45_003375"
     CDS             8646..9374
                     /gene="recO"
                     /locus_tag="OVW45_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCV5527743.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            9386..10117
                     /gene="pdxJ"
                     /locus_tag="OVW45_003376"
     CDS             9386..10117
                     /gene="pdxJ"
                     /locus_tag="OVW45_003376"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCV5527744.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLTDAGIQISLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            10117..10497
                     /locus_tag="OVW45_003377"
     CDS             10117..10497
                     /locus_tag="OVW45_003377"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCV5527745.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            10719..10799
                     /gene="shoB"
                     /locus_tag="OVW45_003378"
     CDS             10719..10799
                     /gene="shoB"
                     /locus_tag="OVW45_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCV5527746.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(10993..11253)
                     /gene="yfhL"
                     /locus_tag="OVW45_003379"
     CDS             complement(10993..11253)
                     /gene="yfhL"
                     /locus_tag="OVW45_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCV5527747.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            11468..12658
                     /locus_tag="OVW45_003380"
                     /pseudo
     CDS             11468..12658
                     /locus_tag="OVW45_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(12843..14030)
                     /locus_tag="OVW45_003381"
     CDS             complement(12843..14030)
                     /locus_tag="OVW45_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCV5527748.1"
                     /translation="MLPRFADIFQQGNHWLNWLEKQPERAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLTEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNAPHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITE
                     TVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHEPLARMR
                     WCG"
     gene            complement(14030..>14367)
                     /locus_tag="OVW45_003382"
     CDS             complement(14030..>14367)
                     /locus_tag="OVW45_003382"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5527749.1"
                     /translation="KPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDSFDDAVECCAD
                     LNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILSGISAGGMYCPECGTVH
                     WPDGVAPPF"
BASE COUNT         3621 a         3607 c         3707 g         3432 t
ORIGIN      
        1 ccgcgcgcca cgccatagca atgccatcgc cagaagaaat atccggattg gtggtgtact
       61 gatacacctt cgacgcaccg ccggttgcca gtaccactgc ttttgcgtgg caggtttcca
      121 ccgtttcttt atttcggttc catacccacg cgcctacaac ccgtcgcgtg cccggcaggc
      181 caattttgtc agagacaatc agatcaactg cgttgctgcg ctccagcaca cgaatattcg
      241 gatggttctg cgccttgctc accagcgtgg attgtacttc tctaccggtg gcgtcggcgg
      301 catgaagaat acgacggtga ctatgtccac cttcacgggt cagatggtag ctttcttcgc
      361 cattcggttg aacgtgggta tcaaacaaaa ccccctggtc aattaaccat tgcacacagg
      421 aacgagcatt gctggcgaca aattcaactg catggcgatc gcaaatacca gccccggcaa
      481 tcaatgtgtc ttccacatgc gagtcaatgc tgtcagtttc atcaaacacg gcggcaatac
      541 cgccctgggc ataaaatgtt gaaccttccg ttaccgggcc tttacttaga acgatgacct
      601 gatgctggtc agccaggcgt agcgccagtg aaagtccggc tgcgccgcta ccaataatca
      661 acacgtcaca tgaatgttca gggagagtat tcattttctt tgtttaattt actaaacatt
      721 gtttggtcag catagcatca tgttgtgcga ataaacacct gctattttaa tatttgttac
      781 agtggctaaa cacgttgacg cagggcggcg agaaaaagag aagttactgg ctggtggagg
      841 attaggtggt gaaataaaaa ggccgttggg ttactcttca ggcagttaaa tgggcatttc
      901 tacacagata atgcgatgtt cagattctgt agacttataa tgatagataa tgatccgtct
      961 acagcatgac aaacaaaaac agatgcgtta cggaacttta caaaaacgag acactctaac
     1021 cctttgcttg ctcaaattgc agctaatgga gtggcgtttc gatagcgcgt ggaaatttgg
     1081 tttggggaga ctttacctcg gatgagcgag cagttaacgg accaggtcct ggttgaacgg
     1141 gtccagaagg gagatcagaa agcctttaac ttactggtag tgcgctatca gcataaagtg
     1201 gcgagtctgg tttcccgcta tgtgccgtcg ggtgatgttc ccgatgtggt acaagaagct
     1261 tttattaaag cctatcgtgc gctggattcg ttccggggag atagcgcttt ttatacatgg
     1321 ctgtatcgga ttgctgtaaa tacagcgaaa aattacctgg ttgctcaggg gcgtcgtcca
     1381 ccttccagtg atgtggatgc cattgaagct gaaaacttcg aaagtggcgg cgcgttgaaa
     1441 gaaatttcga accctgagaa cttaatgttg tcagaagaac tgagacagat agttttccga
     1501 actattgagt ccctcccgga agatttgcgc atggcaataa ccttgcggga gctggatggc
     1561 ctgagctatg aagagatagc cgctatcatg gattgtccgg taggtacggt gcgttcacgt
     1621 atcttccgag cgagggaagc tattgataac aaagttcaac cgcttatcag gcgttgacga
     1681 tagcgggata ctggataagg gtattaggca tgcagaaaga acaactttcc gctttaatgg
     1741 atggcgaaac gctggatagt gagctgctta acgaactggc tcataaccca gaaatgcaga
     1801 aaacctggga aagctatcac ttaatccgtg attcaatgcg gggtgatact cccgaggtgc
     1861 tccatttcga tatctcttca cgcgtgatgg ccgccattga agaagagcca gtacgtcaac
     1921 cggcgacatt gatcccggaa gcccagcctg cgccgcatca atggcacaaa atgccattct
     1981 ggcagaaagt acgtccgtgg gcggcacagc ttacccaaat gggcgtagca gcatgcgtat
     2041 cgcttgcagt tatcgttggc gtccagcact ataatggaca atctgaaacg tcccagcagc
     2101 ccgaaacgcc ggtatttaat acactgccga tgatgggtaa agccagcccg gtaagcctgg
     2161 gagtaccttc tgaagcgacc gcaaacaatg gtcaacagca gcaggtacag gagcagcgtc
     2221 gtcgcattaa tgcaatgttg caggattacg aactgcaacg ccgactccac tctgaacagc
     2281 ttcagtttga gcaggcgcaa acccagcaag ccgctgtaca ggtgccagga cttcaaactt
     2341 taggaacgca atcgcagtaa tgaagcaact ttggtttgcc atgtcattag tgacaggtag
     2401 cctgttattc tctgctaacg cctcggccac tcccgcgtcc ggggcgttat tacagcagat
     2461 gaacctggcc agtcagtcac tgaattacga gctgtcattc atcagcatca ataaacaggg
     2521 tgttgagtct ctgcgttatc gacatgcacg cctcgataac cgtcctcttg cacaattgtt
     2581 gcaaatggat ggcccgcgcc gggaagtggt acagcgcggc aatgaaatca gctattttga
     2641 accgggactt gaaccgttca cgcttaatgg cgattacatt gttgattctc tgccatcgct
     2701 tatctatacc gatttcaaac gcctttctcc ttactacgac tttatctccg tcgggcgcac
     2761 gcgtattgct gatcgtcttt gcgaagtcat tcgcgtggtt gcccgagatg gtacacgcta
     2821 cagctacatc gtgtggatgg acactgaatc gaaattaccg atgagggttg atctccttga
     2881 tcgcgatggt gaaacgctgg aacaatttcg cgtgatttct tttaacgtca atcaggatat
     2941 cagcagcagt atgcagacgc tggcgaaggc aaatttgccg ccgttgcttt ctgttcctgt
     3001 aggtgaaaaa gctaaattca gctggacgcc aacctggttg ccacagggtt ttagcgaagt
     3061 ttccagtagt cgacgtccgc taccgacgat ggacaacatg cctatcgaat cacgtctcta
     3121 ttccgacgga ttattcagct tctcggtaaa cgttaaccgc gctacgccat cgagcaccga
     3181 tcagatgttg cgcaccggac gcagaaccgt cagtacaagc gtacgtgata acgccgaaat
     3241 caccattgtc ggtgaactgc cgccgcaaac ggcgaaacgc attgccgaga atattaagtt
     3301 cggggcagcg caatgatcaa agagtgggct accgtcgtct cctggcaaaa cgggcaggcg
     3361 ctggtcagtt gtgatgttaa agcctcatgc agcagctgcg cttcacgcgc cggttgcggt
     3421 agccgcgtgt tgaataaact tggcccgcaa accacgcata ccattgtcgt accctgtgat
     3481 gaaccgttag tgccggggca aaaagtggaa ttggggatcg ccgaaggcag cctgcttagc
     3541 tccgcattac tggtttatat gtcgccgctg gtgggattat tccttatcgc ttcgctattt
     3601 cagctactct ttgcttctga cgttgcagca ttatgtggtg cgatccttgg cggtattggc
     3661 gggtttctga ttgcgcgcgg ttactcgcgt aagtttgctg cccgggcaga atggcaaccg
     3721 atcatattaa gcgtggcatt gccgcctgac cttgtgcgat ttgaaacatc ttcggaagac
     3781 gcgagccagt gatttcactg gctttgttgc attttgcata acaaatgcga ggacgtttcc
     3841 agataccaca atccatacgg tatgaacatt tcctcgcctc aatgttgtag tgtagaatgc
     3901 ggcgtttcta ttaatacaga cgttaagctc agaacagcga cttctaaaag cctggttaac
     3961 cgggcattaa ggcacaataa tcatacttta tgaagaatat acgtaacttt tcgatcatag
     4021 ctcacattga ccacggtaaa tcgacgctgt ctgaccgtat tatccagatc tgcggtggcc
     4081 tgtctgaccg tgaaatggag gcgcaggttc tcgattccat ggatcttgag cgtgagcgtg
     4141 gcattaccat caaagcgcaa agcgtgacgc tggactacaa agcgtctgac ggcgaaacct
     4201 atcagcttaa ctttatcgac accccgggcc acgtagactt ctcctatgaa gtttcccgtt
     4261 cgctggctgc ctgtgaaggt gcattgctgg tggtcgacgc cgggcagggc gtggaagcgc
     4321 aaaccctggc aaactgctac accgccatgg aaatggatct cgaagtggtg ccagtactga
     4381 acaagattga cctgccggca gccgatcctg aacgcgtggc ggaagaaatt gaagatatcg
     4441 tcggcatcga cgccactgac gcggtgcgct gttcagcgaa aaccggcgtt ggcgtgcagg
     4501 acgttctcga acgtctggtg cgcgacattc cgccgccgga aggcgatccg gaaggcccgt
     4561 tgcaggcact aattatcgac tcatggttcg acaactacct gggcgttgtt tcacttatcc
     4621 gtattaaaaa cggcaccctg cgtaagggcg acaaagtgaa agtcatgagt accgggcaga
     4681 cctataacgc cgaccgtctg ggcatcttca cgccgaaaca ggttgaccgc actgaactga
     4741 aatgtggcga agtaggctgg ctggtatgtg cgattaaaga tatccacggc gctccagtcg
     4801 gcgatacctt aacgctggcg cgtaatccgg cagaaaaggc gctgcctggc tttaagaaag
     4861 tcaaaccgca ggtatacgcc ggtctgttcc cggtaagttc cgacgactat gaagccttcc
     4921 gtgacgcgct gggtaaactc agcctgaatg atgcctcact gttctatgag ccggaaagct
     4981 ccagcgcgct gggctttggt ttccgctgcg gcttccttgg cctgctgcac atggagatca
     5041 tccaggaacg tctggaacgt gaatacgatc tggatctgat caccactgcg ccgaccgtag
     5101 tgtatgaagt tgaaaccacg tcaagggaag ttatctacgt cgacagccca tccaagctgc
     5161 ctgcggtaaa taacatttac gaactgcgcg agccgattgc agagtgtcac atgctgctgc
     5221 cgcaggcata tctcggcaac gttattacgc tgtgcgtaga aaaacgcggc gtgcagacca
     5281 atatggttta ccacggtaat caggtggcgc tgacgtacga gatcccgatg gcggaagtgg
     5341 tgctcgactt cttcgatcgc ctgaaatcta cctcgcgtgg ttatgcgtct ctggattaca
     5401 acttcaaacg cttccaggcg tccgacatgg tacgtgtaga cgtattaatc aacggtgaac
     5461 gtgttgatgc gttggcgctg atcacccacc gtgataattc gcaaaaccgc ggtcgcgagt
     5521 tggtggagaa gatgaaagat ctgatcccac gccagcagtt tgatatcgcc attcaggcag
     5581 cgattggtac gcacattatt gcgcgatcca ccgtgaaaca gctgcgtaaa aacgtactgg
     5641 ctaaatgtta tggcggcgat atcagccgta agaaaaagct gctgcagaag cagaaagaag
     5701 gtaagaaacg catgaagcag atcggtaacg tcgagctgcc gcaggaagcg ttcctcgcca
     5761 ttctgcacgt cggcaaagac aacaaataac ccttaggagt tggcatggcg aatatgtttg
     5821 ccctgattct ggtgattgcc acactggtga cgggcatttt atggtgcgtg gataaattct
     5881 ttttcgcacc taaacggcgg gaacgtcagg cagcggcgca ggcggctgcc ggtgactcac
     5941 tggataaagc aacgttgaaa aaggttgcac cgaagcctgg ctggctggaa accggagctt
     6001 ctgtttttcc ggtgctggct atcgtattga ttgtacgttc gtttatttat gaaccgttcc
     6061 agatcccgtc aggttcgatg atgccgactc tgttaatcgg tgattttatt ctggtagaga
     6121 agtttgctta tggcattaaa gatcctatct accagaaaac gctgatcgaa accggtcatc
     6181 cgaaacgcgg cgatatcgtg gtctttaaat atccggaaga tccaaagctt gattacatca
     6241 agcgcgcggt gggtttaccg ggcgataaag tcacttacga tccggtctca aaagagctga
     6301 cgattcaacc gggatgcagt tccggccagg cgtgtgaaaa cgcgctgccg gtcacctact
     6361 caaacgtgga accgagcgat ttcgttcaga ccttctcacg ccgtaatggt ggggaagcga
     6421 ccagcggatt ctttgaagtg ccgaaaaacg aaaccaaaga aaatggaatt cgtctttccg
     6481 agcgtaaaga gacactgggt gatgtgacgc accgaattct gacagtgccg attgcgcagg
     6541 accaggtggg gatgtattac cagcagccag ggcaacaact ggcaacctgg attgttccgc
     6601 cgggacaata cttcatgatg ggcgacaacc gcgacaacag cgcggacagc cgttactggg
     6661 gctttgtgcc tgaagcgaat ctggtcggtc gggcaacggc tatctggatg agtttcgata
     6721 agcaagaagg cgaatggccg acaggtgtgc gcttaagtcg cattggcggc atccattaat
     6781 agcccatctt cgttcacgtt gtcgccgtta tggcgacaac gtgaattatt tatgagataa
     6841 atctcccgtg gctaacgaca tcccccgtcg ttgtgtatag aatattcccc cgaagtttaa
     6901 ggttggcacc tccaggttgc cacggcacac gaaacagcgt tggtccccat ataccggtaa
     6961 actgaaactg cagcgaagca gttagcagag ccatgtatat caggtctgtt tcgtgtgctg
     7021 aattgttgac gcatttattt attggtatcg catgaacccc atcgtaatta atcggcttca
     7081 acggaagctg ggctacactt ttaatcatca ggaactgttg cagcaggcat taactcatcg
     7141 tagtgccagc agcaaacata acgagcgttt agaattttta ggcgactcta ttctgagcta
     7201 cgttatcgcc aatgcgcttt atcaccgttt ccctcgtgtg gatgaaggcg atatgagccg
     7261 gatgcgcgcc acgctggtcc gtggcaatac gctggcggaa ctggcgcgcg aatttgagtt
     7321 aggcgagtgc ttacgtttag ggccaggtga acttaaaagc ggtggatttc gtcgtgagtc
     7381 aattctcgcc gataccgtcg aagcattaat tggtggcgta ttcctcgaca gtgatattca
     7441 aaccgtcgag aaattaatcc tcaactggta tcaaactcgt ttggacgaaa ttagcccagg
     7501 cgataaacaa aaagatccga aaacgcgctt gcaagaatat ttgcagggtc gccatctgcc
     7561 gctgccgact tatctggtag tccaggtacg tggcgaagcg cacgatcagg aatttactat
     7621 ccactgccag gtcagcggcc tgagtgaacc ggtggttggc acaggttcaa gccgtcgtaa
     7681 ggctgagcag gctgccgccg aacaggcgtt gaaaaaactg gagctggaat gagcatcgat
     7741 aaaagttact gcggatttat tgccatcgtc ggacgtccga acgttggcaa atccacattg
     7801 ttgaacaaac tgctggggca gaagatctcc atcacctccc gcaaagcgca aacaactcgt
     7861 caccgcattg tggggatcca tactgaaggc gcgtatcagg cgatctacgt tgatacccct
     7921 ggcctgcata tggaagaaaa acgcgctatt aaccgcctga tgaacaaagc ggcgagcagc
     7981 tctattggcg acgttgagct ggtgattttt gtcgttgaag gcacccgctg gacgccggac
     8041 gacgaaatgg tgctcaacaa actgcgcgat ggcaaagcgc cggtgatcct cgcggtgaac
     8101 aaagtggata acgtgcagga aaaagccgat ctgctgccgc acttacagtt cctggcaagt
     8161 cagatgaact tcctcgatat cgtgccgatc tctgctgaaa ccgggctgaa tgtcgacact
     8221 atcgcagcaa tcgtgcgtaa gcatctgcct gaagcgaccc atcacttccc ggaagattac
     8281 atcaccgatc gctcacagcg ttttatggcg tctgaaatca tccgcgaaaa actgatgcgt
     8341 ttcctcggcg ctgaactgcc gtactccgtg accgtggaga tcgaacgttt cgtctctaac
     8401 gaacgcggtg gttatgacat caatggtttg attctcgttg agcgtgaagg gcagaagaag
     8461 atggttattg gcaacaaagg ggccaagatc aaaaccatcg ggattgaagc gcgtaaagac
     8521 atgcaggaaa tgttcgaagc gcctgtccac cttgaactgt gggtaaaagt gaaatccggt
     8581 tgggccgacg acgaacgcgc actgcgcagt ctcggttacg ttgacgatct ttaagagtca
     8641 ctccgatgga aggctggcag cgcgcatttg tcctgcatag tcgcccgtgg agcgaaacca
     8701 gcctgatgct ggacgtcttc acggaggaat cgggtcgcgt gcgtctggtt gccaaaggcg
     8761 cacgctctaa acgctctact ctgaaaggtg cattacagcc tttcacccct ctcttgctac
     8821 gtttcggcgg gcgtggcgaa gtcaaaacgc tgcgcagtgc tgaagctgtc tcgctggcgc
     8881 tgccattaag cggtatcacg ctttacagcg gtctgtacat caacgaactt ctctcccgcg
     8941 tactggaata cgagacgcgc ttctctgaac tctttttcga ttacttgcac tgcattcagt
     9001 ctcttgcagg tgccactggt acgccagaac ccgcgctgcg ccgctttgaa ctggcactgc
     9061 tcgggcatct gggctatggc gtcaatttta cccattgcgc gggtagcggc gagccggtag
     9121 atgacaccat gacgtatcgt tatcgcgaag aaaaagggtt tatcgcaagc gtcgttatcg
     9181 acaataaaac gttcaccgga aggcagttaa aagcgttaaa cgcgcgggaa tttcctgacg
     9241 cagacacact acgcgccgcg aaacgcttta cccgcatggc gcttaagccg tatcttggcg
     9301 gtaaaccatt aaagagcagg gaactgttcc ggcagtttat gcctaagcga acggtgaaaa
     9361 cacattatga atgatgagga ttgtcatggc tgaattactg ttaggcgtca acattgacca
     9421 tattgcaacg ctgcgtaacg cacgcggtac tgcttacccg gatccggtgc aggcggcgtt
     9481 tattgccgag caggcgggag cggacggcat taccgtgcat ttacgtgaag atcgccgcca
     9541 cattactgac cgcgatgtgc gcatcctgcg gcagacgctg gatacccgaa tgaatctgga
     9601 gatggcagtg accgaagaga tgctggcgat cgccgttgag acgaagccac atttttgctg
     9661 cctggtaccg gaaaagcgtc aggaagtaac aaccgaaggc ggcctggacg ttgcagggca
     9721 gcgtgaaaaa atgcgcgatg cctgcaaacg tctgacagat gccgggattc agatttctct
     9781 ctttatcgac gccgatgaag agcagatcaa agctgcggca gaggttggcg caccgtttat
     9841 cgagatccac accggttgct atgctgatgc caaaactgac gccgaacagg cgcaagagct
     9901 ggtgcgtatc gccaaagccg cgacctttgc cgctagtctc ggtctgaaag ttaacgccgg
     9961 acacggtctg acctatcaca acgtgaaagc cattgccgcc atccctgaga tgcatgaact
    10021 gaatatcggt catgccatta ttggccgtgc agtgatgacc ggactgaaag atgcggtggc
    10081 agaaatgaag cgtctgatgc tggaagcgcg tggctaatgg caatattagg tttaggcacg
    10141 gatatcgtag agatcgctcg catagaagca gtgatcgccc gatccggtga gcgcctggca
    10201 cgccgcgtat taagcgataa cgaatgggca atctggaaaa cgcaccacca gccggtgcgt
    10261 tttctggcga agcgttttgc tgtgaaagaa gccgcagcaa aagcgtttgg caccgggatc
    10321 cgcaatggtc tggcgtttaa tcaatttgaa gtattcaatg atgagctcgg caaaccacgg
    10381 ctacggctat ggggtgaggc attaaaactg gcggaaaagc tcggcgttgt aaatatgcat
    10441 gtaacgctgg cagatgaacg ccactatgct tgtgccacgg taattattga aagttaaaat
    10501 aataccgttc tctttaaaaa gtaaaacccc cgaactggta aggaacgggg gttttacttt
    10561 ttaactttaa ttttgtgcag catgcaccct caacaacagc gattatggat cagtgtactt
    10621 atcttttaca agcaacttgc aatctttagc ataaaaactc gagcctttac gaagaaagca
    10681 atactgaacg gctgcattcc ttgtttaagg aaaaacgaat gactgattgc cgatacctga
    10741 ttaaacgggt catcaaaatc atcattgctg ttttacagct gatccttctg ttcttataac
    10801 acaaggaaac gtacttaagg tgcgtccggt gaaccaatcg gacgcgcctt taataactat
    10861 aaataagtgt cttggcagat actatataaa ttaacttagt gaatgattat gctaatgtca
    10921 tcaattaaat aaatataatg gcgttaaggc ttcccagtaa tataattaat actctacttc
    10981 cagagtagaa tattaaattt tatccgcgtg gtgcatcagc acaaatttat cccacaactg
    11041 ttcttctgtc tcgacatgcg ccggatcttt cacaatagta ttggggatcg ggcacacctt
    11101 ctggcaggtt ggtgtctcgt agtgccctac gcattcggta cacttatcgc tgttaatctc
    11161 gtagatatga tctcccattg aaatcgcctc attcgggcat tcgggttcac acatatcaca
    11221 attaatacag cgtttagtaa ttagtaaaga catttcaatg gattaccgtt aaatcatttt
    11281 aaaatcagta agttgtatcg agtttgtatg ctttactgtc attaacttac tgtatgttga
    11341 tccagtgtat ttaaccttga taaactcagt ccagcaacac aaaaccgcaa tacattgcat
    11401 tttgtcccgt agaaaagact tgtatgtgtg agcttgtttt ctgcgcctac gcagataagg
    11461 attgagaatg ccgcgcactg taacacataa tccggatagc cccaataatg acgatgtttt
    11521 agccgcatct gaaaaatggg acgcctgtaa acccccctat accagcgcac acatgaaaat
    11581 ctgtgttgct gccgccaaaa tcatcctcgc tgcttccggc gtggctcgcc gttccaaata
    11641 cgaaaaagag aactatctcc gtatcgattt cagcaaagcc ggtaaggtta cattttacgc
    11701 cgagtttcca aaaaagatgg gcctcaaagg taaaaagctc ggcgagtggc cggagctcgc
    11761 tatccagctg gcgcgcgaaa aagcactagg tatggctgac ggtggactgc gggcagagtc
    11821 cgtacatgca gcgctggaaa tgtaccggga tgacctcaaa gccaaagtag cccggcagaa
    11881 gctgagcccg gacagtttca caacctacgg ggtgcgtatc gaccggatta aagcaacgtt
    11941 cggcgagcgt gaggtgttca gcgacgtaac atacaatcgg ctggtggaag tgctggacga
    12001 gtggatcgcc actcgctcga acaataacgc cctggagttg tttgccgagc tccgtcggtt
    12061 ctggaagttc tgcgcaccta ctctttgcaa cggccgcaat gttgccgcca gtctgccaga
    12121 tgattatgtt tcctcccgcg tacagaaacc tacccccaca cggcttttta ccgatattga
    12181 atcaatcgcc cgactctggc tcaatgttgc tgcctgcacc tctgtacacc agaagaatgc
    12241 tgttcgcttc atgatcatca ctggtgttcg tccgattaat gtccataacc tgcgctggga
    12301 ctacgtttac gaggaggctg gtgaaattgt ttatccggaa ggggttatcg gcatgcgagg
    12361 ggctatgaaa acacaaaagg ctttccgcct gccgataacg cctgagatcc ggcggattat
    12421 cgacgagcag aaagcctggc gtgattcagt tcctgagtgc aacagggatt atgtattttt
    12481 gcagccacgt gatccaatgc agccattttc aaaacgatca ctggataagc tggtgaaaac
    12541 atacagcccg gacggggctg taaaaggaat aaaacatgat gggactgtta aagggaaaga
    12601 cggtgcattt aatacgatgt gccgtaaatt ccttaagagc aatgttattg ccttgatcga
    12661 taggaattaa aaccccaaaa agattaaaaa aacaccacaa aacggatgtt tcttcaacac
    12721 cacttttgct catatgaacg gaaccgacga ttaaactgga tggctctgat tgattcaggg
    12781 tatgaatggc ggttttttgc tccgtttccc tcaaaatgga cgcaactttc cctctgcggc
    12841 tctcagccgc accaccgcat ccgggccagc ggctcatgca tcaggacctg ctctgccaga
    12901 cggtagcccc gcttcagccc cgtaaaacgc atctgactcc cgcacagcac gcacttcagc
    12961 gggtcaacct tcagtaacct ctgatacatc cctctccagg tgatttgcat cgccgttttt
    13021 ctcactgtct ccgttatgat gtacaccact tcttccagta accgccgttt cgccggactc
    13081 aaaaaaccgt agtacctcac catacggaac cccttatccg ccacatgcca ggagaacctt
    13141 tccatgaact catctccact catcaacagg tattcttccc gttttgttcg gtgactgttg
    13201 taacgcagac cgatttcatc ctgaccggca taatgctcca gacgactcat cggcaccggt
    13261 ggctttttca ggtaagagcc aaagtacacc gccacatggg gggcattatc catcacccgg
    13321 gatacgttga cattccagcc acggcggtaa tgcgtgtcca ggaagcgatt ccattcccgt
    13381 ttactgcttc cttctgctgc cagcgcatcc ggcatcacca ggtcagggta tttccgtgac
    13441 agcaaccgtg ttatccggta gcgccacatg ctcatcacct tacgggcgta aaaatgaaga
    13501 tttttccagg tgtggcccga cgtcacacca ccggcagttg tcgataaatg gatatgcgga
    13561 tgccactgct ggtcacgccc ccatgtgtgg atcaccgtga atatccccgg ctccacatct
    13621 gcctgatggc agatttccag tatcacatcc gctgcaatgc ggctcatctc tgtcagtaac
    13681 caccggttgt ggaacaccag ggaccagtac tggcagggaa gtgtgaacac aatatgctgc
    13741 cacgggcagt cggggaccag gctcagcaga tactgtatcc actgtgcgcc agccttcacc
    13801 ccgcagtgcg ggcaggagcg gcttttacac cggaagcaga ccttttttgt atggcaacag
    13861 tccggtgatg aacagcacca ctgtgtatac cccatcagtg tggtcccgca cgccatgatt
    13921 ttggtcaccg actcaatcac caccggacgt actgcccttt ccggctgctt ctccagccag
    13981 ttaagccagt ggtttccctg ctgaaagata tcggcaaaac ggggaagcat cagaagggcg
    14041 gggcgactcc gtccggccag tgaaccgtgc cacactccgg gcagtacata ccgccggcgc
    14101 tgataccgga aagaatggtc gcaaattccc gctccgtgca gcgggcgatt tccggatacc
    14161 cttcgtcatc aacacgtaca aaccagaaga ccagcttttt gtttcccgca tccacaaaga
    14221 acggaatatt caggtctgcg cagcattcaa cggcatcgtc aaaactatca aagcgcagaa
    14281 cttctgcgtc ttcttcgtca aaaaaatcat cttcgtgaag cttcacgaca tagcggggaa
    14341 gtttgcttct ttgagaggcg ggtttac
//
LOCUS       DAKISA010000105        14326 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.105, whole genome shotgun sequence.
ACCESSION   DAKISA010000105 DAKISA010000000
VERSION     DAKISA010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14326
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.105"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            75..2216
                     /gene="ldcC"
                     /locus_tag="OVW45_003383"
     CDS             75..2216
                     /gene="ldcC"
                     /locus_tag="OVW45_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCV5527750.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            2273..2662
                     /locus_tag="OVW45_003384"
     CDS             2273..2662
                     /locus_tag="OVW45_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCV5527751.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            2727..4025
                     /gene="tilS"
                     /locus_tag="OVW45_003385"
     CDS             2727..4025
                     /gene="tilS"
                     /locus_tag="OVW45_003385"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCV5527752.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(4074..4334)
                     /gene="rof"
                     /locus_tag="OVW45_003386"
     CDS             complement(4074..4334)
                     /gene="rof"
                     /locus_tag="OVW45_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCV5527753.1"
                     /translation="MSMNDAYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(4321..4521)
                     /locus_tag="OVW45_003387"
     CDS             complement(4321..4521)
                     /locus_tag="OVW45_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCV5527754.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            4687..5232
                     /locus_tag="OVW45_003388"
     CDS             4687..5232
                     /locus_tag="OVW45_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCV5527755.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LVWLKYADERLQFTRGLCADYEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAKV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            5229..5590
                     /gene="arfB"
                     /locus_tag="OVW45_003389"
                     /pseudo
     CDS             5229..5590
                     /gene="arfB"
                     /locus_tag="OVW45_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
     gene            5664..6374
                     /gene="nlpE"
                     /locus_tag="OVW45_003390"
                     /gene_synonym="cutF"
     CDS             5664..6374
                     /gene="nlpE"
                     /locus_tag="OVW45_003390"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCV5527756.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(6529..7353)
                     /locus_tag="OVW45_003391"
     CDS             complement(6529..7353)
                     /locus_tag="OVW45_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCV5527757.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSSYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(7406..9124)
                     /gene="proS"
                     /locus_tag="OVW45_003392"
     CDS             complement(7406..9124)
                     /gene="proS"
                     /locus_tag="OVW45_003392"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCV5527758.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="OVW45_003393"
     CDS             complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="OVW45_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCV5527759.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="OVW45_003394"
     CDS             complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="OVW45_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCV5527760.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASKQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTLGCYRQAVCIGSALNITAK"
     gene            complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="OVW45_003395"
     CDS             complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="OVW45_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCV5527761.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(11316..11969)
                     /gene="metI"
                     /locus_tag="OVW45_003396"
     CDS             complement(11316..11969)
                     /gene="metI"
                     /locus_tag="OVW45_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCV5527762.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(11962..12993)
                     /gene="metN"
                     /locus_tag="OVW45_003397"
     CDS             complement(11962..12993)
                     /gene="metN"
                     /locus_tag="OVW45_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCV5527763.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            13181..13756
                     /gene="gmhB"
                     /locus_tag="OVW45_003398"
     CDS             13181..13756
                     /gene="gmhB"
                     /locus_tag="OVW45_003398"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCV5527764.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFEMLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
     gene            14120..>14326
                     /locus_tag="OVW45_003399"
     rRNA            14120..>14326
                     /locus_tag="OVW45_003399"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         3500 a         3606 c         3806 g         3414 t
ORIGIN      
        1 cacttcggtg gccctttttt atcgccacgg tttgagcagg ctatgattaa ggaaggattt
       61 tccaggagga acacatgaac atcattgcca ttatgggacc gcatggcgtc ttttataaag
      121 atgagcccat caaagaactg gagtcggcgc tggtggcgca aggctttcag attatctggc
      181 cacaaaacag cgttgatttg ctgaaattta tcgaacataa ccctcgaatt tgcggcgtga
      241 tttttgactg ggatgagtac agtctcgatt tatgcagcga tatcaatcaa cttaatgaat
      301 atctcccgct ttatgccttc atcaacaccc actcgacgat ggatgtcagc gtgcaggata
      361 tgcggatggc gctctggttt tttgaatatg cgctggggca ggcggaagat atcgccattc
      421 gtatgcgtca gtacaccaac gaatatcttg ataacattac gccgccgttc acgaaagcct
      481 tgtttaccta cgtcaaagag cggaagtaca ccttttgtac gccggggcat atgggcggca
      541 ccgcatatca aaaaagcccg gttggctgcc tgttttatga ttttttcggc gggaataccc
      601 tcaaggctga tgtctctatt tcggtcaccg agcttggttc gttgctcgac cacaccgggc
      661 cacacctgga agcggaagag tacatcgcgc ggacttttgg cgcggaacag agttatatcg
      721 ttaccaacgg aacatcgacg tcgaacaaaa ttgtgggtat gtacgccgcg ccatccggca
      781 gtacgctgtt gatcgaccgc aattgtcata aatcgctggc gcatctgttg atgatgaacg
      841 atgtagtgcc agtctggctg aaaccgacgc gtaatgcgtt ggggattctt ggtgggatcc
      901 cgcgccgtga atttactcgc gacagcatcg aagagaaagt cgctgccacc acgcaggcac
      961 aatggccggt tcatgcggtg attaccaact ccacctatga tggcttgctc tacaacaccg
     1021 actggatcaa acagacgctg gatgtcccgt cgatccactt cgattccgcc tgggtgccgt
     1081 acacccattt tcatccgatc taccagggga aaagtggtat gagcggcgag cgtgttgcgg
     1141 gaaaagtgat cttcgaaacg caatcgaccc acaaaatgct ggcggcgtta tcgcaggctt
     1201 cgctgatcca cattaaaggc gagtatgacg aagaggcgtt taacgaagcc tttatgatgc
     1261 ataccaccac ctcgcccagt tatcccattg ttgcttcggt tgagacggcg gcggcgatgc
     1321 tgcgtggtaa tccgggcaaa cggctgatta accgttcggt ggagcgggcg ctgcattttc
     1381 gcaaagaggt ccagcggttg cgggaagagt ctgacggttg gtttttcgat atctggcaac
     1441 cgccgcaggt ggatgaagcc gaatgctggc ccgttgcgcc aggcgaacag tggcacggct
     1501 ttaacgatgc ggatgccgat catatgtttc tcgatccggt taaagtcact attttgacac
     1561 cggggatgga cgaacagggc aatatgagcg aggaggggat cccggcggcg ctggtggcaa
     1621 aattcctcga cgaacgtggg atcgtagtag agaaaaccgg cccttataac ctgctgtttc
     1681 tctttagtat tggtatcgat aaaaccaaag caatgggatt attgcgtggg ttgacggaat
     1741 ttaaacgctc ttacgatctc aacctgcgga tcaaaaatat gctacccgat ctctatgctg
     1801 aagatcccga tttctaccgc aatatgcgta ttcaggatct ggcgcaaggg atccataagc
     1861 tgattcgtaa acacgatctt cccggtttga tgttgcgggc attcgatact ttgccggaaa
     1921 tgatcatgac gccacatcag gcatggcaga gacagattaa aggtgaagta gaaaccattg
     1981 cgctggaaca actggtcggt agagtatcgg caaatatgat cctgccttat ccaccgggcg
     2041 taccgctgtt gatgccagga gaaatgctga ccaaagagag ccgcacggta ctcgattttc
     2101 tactgatgct ttgttccgta gggcaacatt accccggttt tgaaacggat attcacggcg
     2161 cgaaacagga cgaagacggc gtttaccgcg tacgagtcct aaaaatggcg ggataacttg
     2221 ccagagcggc ttccgggcga gtaacgtgct gttaacaaat aaaggagacg ttatgctggg
     2281 tttaaaacag gttcaccata ttgcgattat tgcgacggat tatgcggtga gcaaagcttt
     2341 ctactgcgat attcttggtt tcacgctgca aagcgaagtc tatcgcgaag cgcgcgactc
     2401 gtggaaaggg gatttggcgc ttaatgggca atatgtgatt gagcttttct cttttccgtt
     2461 cccgccggag cgccccagcc gaccggaagc ttgcggcctg cgtcatctgg cttttagcgt
     2521 tgatgatatc gatgcggcag tggcgcacct tgaaagccat aacgtgaagt gtgaagccat
     2581 ccgtgtcgat ccatacacgc aaaaacgctt cacgttcttt aacgatccgg acgggctgcc
     2641 gttggaactg tatgagcagt aaggcttgtc atcgccgcat ttgcccggta acgtgccggg
     2701 cattgctact gtaaaatcgc accatcatga cactcacgct caatagacaa cttctcacct
     2761 cacgccagat tctggtggcc tttagcggcg ggcttgactc caccgttctg ctgcatcagt
     2821 tggtgcagtg gcggacggaa aatccgggcg tcactctacg cgctatccat gtgcatcatg
     2881 gtttaagtgc taatgccgat gcctgggtta ggcattgcga gaacatctgc caacagtggc
     2941 aggtgccgct ggtggtcgag cgcgtgcaac ttgcgcaaga aggactgggc attgaggccc
     3001 aggcgcggca ggcgcgttat caggcatttg ctcgtaccct gttacccggt gaagtgctgg
     3061 tcaccgcgca acatctcgac gatcaatgtg aaacctttct gctggcgcta aaacgcggta
     3121 gcggccctgc cgggctttcg gctatggggg aagtttcgga atttgccgga acacggctta
     3181 tccgcccgtt gcttgcccgc acgcgggggg aactggagca gtgggcattg gcgcatggtt
     3241 tacgctggat tgaagacgaa agtaatcagg acgactcata cgatcgtaac tttctgcgcc
     3301 tgcgtgtagt gccgttattg cagcagcgtt ggccgcattt tgccgaagca acggcccgca
     3361 gcgccgcact ttgtgctgaa caagagagtc tgttggatga actgctggcg gatgatttag
     3421 cacactgcca aacgtcgcag gggacgctgc agattgcgcc aatgctggcg atgagtgatg
     3481 cccgtcgcgc ggcgattatc cgccgctggc tggcagggca gaatgcaccg atgccttccc
     3541 gcgacgcgct ggtgcggatc tggcaggaag tggcgctggc gcgggaagat gcctccccct
     3601 gtttacgttt tggcgcgttt gaaatacggc gctatcagtc gcaactgtgg tggattaaat
     3661 ccgtcactgg gcaaagcgaa accattgtgc cgtggcagac gtggctacaa ccgctggaat
     3721 taccggcggg gctgggaagt gtacagctta ctgcgggagg cgatattcgc cctccgcgtg
     3781 cagacgaagc ggtcagcgtg cgtttcaaag cgccaggatt gctgcatatt gtcgggcgta
     3841 acggcggacg taagctaaag aaaatctggc aagagctggg cgtgccgccg tggctacgtg
     3901 acaccacgcc actgctgttt tatggcgaaa cgctgattgc ggcggcaggg gtatttgtga
     3961 cgcaagaagg tgtggctgaa ggtgagaatg gcgtaagttt tgtctggcag aaaacgctta
     4021 gttaagtgaa agccggataa gacgcatcaa acgtcgcatc cggcgaaagt caatcaggat
     4081 tcgcttacca ccaccgtacc gatttccggg tggctaaagc tggtaatttt atccagacgc
     4141 agctcgcggg tttcgccagc ggcctcgacg accagatact ccacattttt gcgggagact
     4201 aaatcactgg cttttgcctg caatttttcg ccatctttca gctcaagtgt cagcattaaa
     4261 tgatgctggc aggcgagctc aagattatcg taatcatcac aattgattgg ttgatacgca
     4321 tcattcattg acataatcgc tcaccagtaa gtttgccgca gcgtatgctg ctttttccct
     4381 gacagcctca gaaagggcgt cgtcggcagc catttcattc agcactttca aaacgcagcc
     4441 cagcgcgtcc ggaacgtatc ctaagtctcc gctggcgatt tccgcgtacc gcttgcgtat
     4501 taactcacaa tatttttcca catgccctcc tgtcagcact ctgacttaac cgtggatgca
     4561 agtctaagcc tacgaagata aactctgttt cgcaaggtga ctataccaca ctcatttctg
     4621 caatatcagc gccgcaactg cacgtattcc gttacaatgg cctcctgatt cgaaaggagt
     4681 tttcttatgg cgcttaaagc gacaatttat aaagcgacgg ttaatgtggc cgatctcgac
     4741 cgcaaccagt ttctcgatgc ctctctgacg ctggcgcgcc acccttcaga aacccaggag
     4801 cgtatgatgc tgcgcctgct ggtgtggctg aaatatgctg atgaacgtct gcaatttacc
     4861 cgtggtttgt gtgccgatta tgagccggaa gcgtggctgc gtaacgatca cctgggcatt
     4921 gatttgtgga ttgagctggg gctgccggat gagcggcgga ttaagaaagc ctgcacccag
     4981 gccgcaaaag tggcgctgtt tacctataat agccgggcgg cgcaaatctg gtggcagcaa
     5041 aatcagagca aatgtgtgca gtttgccaat ctttccgtct ggtatctgga cgatgaacaa
     5101 ctggcgaaag taagcgcctt tgccgatcgt accatgacgc tgcaggcaac gattcaggat
     5161 ggcgtgatct ggttatcgga tgataagaat aatctggaag tgaacttaac cgcctggcaa
     5221 caaccttcat gattgtgatt tcccgacatg ttgctattcc cgatggtgag cttgagatca
     5281 ccgccattcg tgcgcagggc gcgggcgggc agcatgttaa taagacctca acggctattc
     5341 atctgcgttt tgacattcgg gcgtccagcc tgccagagta ttacaaagag cgtctgctcg
     5401 ccgccagcca tcatttgatc agcatgatgg cgtaattgtc attaaggcac aggaataccg
     5461 cagtcaggaa ctgaaccgcg aagcggcgct ggcccggctg gtggcagtga ttaaagattt
     5521 aacaacagaa caaaaagccc gacgacccac gcggcccacc cgtgcatcga aagagcgcag
     5581 gctggcatag aaagcacaaa aatcaagcgt gaaggcgatg cgcggcaaag tacgcagcgg
     5641 tcgggaataa aaagaaggaa tggatggtga aaaaagcgat agtgacagcg atggctgtaa
     5701 tcagcctctt tactctgatg ggatgtaata atcgggccga agtcgatacg ctttctccgg
     5761 cgcaggctgc cgaactaaaa ccgatgccgc aaagttggcg cggcgtgctg ccgtgtgccg
     5821 attgcgaagg gatcgaaacc tctctgttcc tcgaaaaaga cggaacatgg gtgatgaatg
     5881 agcgttatct cggtgctcgt gaagaacctt cctccttcgc ttcctacggt acatgggcgc
     5941 gaaccgctga caagctggta ttaaccgaca gcaaaggtga aaagtcatat tatcgggcga
     6001 aaggcgatgc gctggagatg ctcgatcgtg aaggcaatcc aattgaatcg cagttcaact
     6061 atacgctgga accggcacaa tccagtttac ctatgacgcc gatgaccctg cgaggcatgt
     6121 atttttatat ggctgatgcg gcgaccttca ctgattgcgc gaccggaaaa cgtttcatgg
     6181 tagcgaataa cgcagagctg gagcgtagct acctggctgc gcgcggtcac agtgaaaaac
     6241 cggtgttact gtcagtagaa ggtcacttta cgcttgaggc taatccggat actggtgcgc
     6301 caaccaaagt attagcgccc gatacggcgg gtaaatttta cccgaataag gattgcagta
     6361 gtttggggca gtaaacctgc ttaaggcaga aacaaacgca aaattgcctg atgcgctgcg
     6421 cttatcaggc ctacagcata taatgcaaca ttttgaatta gcacgatttt gtaggccgga
     6481 taaggcgttc acgacgcatc cggcattttg tgcacgaggc tggctttatc actgagtcag
     6541 cccaacaaag cgacccgcct tgatataaat ccctggcttc aggtgcccaa catactgtaa
     6601 ctgcgtttct ggcttaaacg cagagacatc gccagtacgc atatgcatca gatcggcagg
     6661 gctaatccag cctgattgcg ccagcgtaag cggatgcccg gctttggcaa atgcatcagt
     6721 gacaaattcc gaacaaaacc acgacttttt gtctccttcg cccacactgc ttaactgcgc
     6781 tttcgccagg ccgctgacgc actgttggcg aaaatcctcg gagaacggat tcagtgagca
     6841 catctggcga gtcaccataa agggaataaa ttcgacaatg ccgcgatagt tataactgct
     6901 atctttgatt ttattggcaa acgcggtgat atctgtggct tgttgcgggg taagatccgg
     6961 gactcgtaag acgaaaagct tatcactatg cttcatcgct tttttaaggg aaacgatctg
     7021 gacgccagcg cctgtcgctt ctgcaacgtt attatcaccc agatagattg caacgtgact
     7081 cacagaggaa gtgctgaaga cgcggattcc aaatgaggtt accccaaggc ttgaggagaa
     7141 cagcaaatcg ccgggtttga gatcaggtac cgttatttct tttattgatt gttcggtgaa
     7201 agagctttga tgctggaatt tgacagccca tgtttttgcc tcggcatcta ctgctgtggc
     7261 tgatgggtct ggctggctga tatcaacggt acaagccgaa agtaaaagaa agctggggag
     7321 aagcaggcgg cagtacgcct ttggtttatc cattttatac aatccatgta aaaaagggcc
     7381 ctgaaattca ggaccctttc tggcatcagc ctttaatctg tttcaccaga tattcgacga
     7441 tgtcaccagt cttaattaac tgtttctcgc cgttgcgacg atatttatat tcgatatcgt
     7501 cgttgtcgag gttacggtcg cccagcacaa tagtgtgcgg aataccgatc agttccatat
     7561 cagcaaacat cacgcccgga cgctctttgc ggtcatccag cagcacttcg ataccttgtg
     7621 cacgcagttc gctgtacagt ttctcagcaa gctcttgtac gcggaaggat ttgtgcatgt
     7681 tcatcggcag aatcgccacc tggaacggcg cgatagcgtc aggccatacg atgccgcgtt
     7741 cgtcgtagtt ctgctcaatc gccgcagcta ccacacgcgt taccccgata ccgtagcaac
     7801 ccatcgtcag gatttggtta cggccatctt caccctgtac ggaggctttc agtgcttcgg
     7861 agtacttggt acccagctgg aagatgtgac caacttcgat accacgtttg atcagcagcg
     7921 taccctggcc atccgggctt ggatcgccag ccaccacgtt acggatatct gcaacttccg
     7981 gggtagcgac atcgcgatcc cagttgatac cgaagtagtg tttaccatcg atgttagcac
     8041 cagcagcgaa atcactcatc gccgcaacgg tacggtcaat caccaccgga atcggcatgt
     8101 ttaccggacc cagtgaaccc ggaccggctt taaccacagc acgaatttct tcttcggtcg
     8161 cgaaagtcag cgggcttgca acctgcggca gtttttctgc tttaacttcg ttcagctcgt
     8221 gatcaccgcg caccagcagc gcaaccagcg ggaagctgct gccttcaacc gctttaacca
     8281 gcagagtctt aaccgttttc tcaatcggca gattgaactg ttcaaccagt tccgcgatgg
     8341 ttttcgcgtt cggcgtatca accagcgtca tttcctgggt agcagcagcg cgcggttctt
     8401 tcggcgcgat agcttctgcc agttcaatgt tcgctgcata gtcagaggtg tcggagaaga
     8461 ccacatcgtc ttcaccgctc tgcgccagca cctggaattc gtgagaggcg ctgccgccga
     8521 tagaaccggt gtcggcttgc acggcgcgga aatccagccc catgcggctg aagattttgc
     8581 tgtaggccgc atacattgca tcgtaggttt cctgcaggga ttcctgagaa gtatggaaag
     8641 agtaagcatc tttcatcagg aattcgcggg aacgcatgac gccgaaacgc ggacgcactt
     8701 cgtcgcggaa cttggtctgg atctgataga agttcagcgg cagctgtttg taagagctaa
     8761 gctcgttacg aatcaggtca gtgataactt cttcatgagt tgggccgagt acgaacggac
     8821 gctcgccacg gtcaacaaaa cgcagcagtt ccggaccgta ctgttcccaa cgaccactct
     8881 cttgccacaa atcggctggc tgaaccaccg gcatcgacac ctcgattgca ccggcgttgt
     8941 tcatctcttc acgcacgatg ttttcgactt ttttcagaac gcgcacgccg gtcggcagcc
     9001 aggtatataa cccggaggcc agcttgcgga tcatcccggc gcgcagcatc agctgatggc
     9061 tgatcacctc ggcgtcggca ggtgtctcct tgagagtgga gagcaggtat tggctagtac
     9121 gcatgttgtt acggttccag ttggaaggta gaacaggctc aaggcgagcc tgggacaaaa
     9181 aaagtgattt agtttaccag tgcaaaagaa atgtcaaaag agaagggcgt gaatttaacg
     9241 cggttccagc gcaaagactt caaaacctgc gtcggtgacg cgccagcgaa cgttaaaatc
     9301 atgtagccag acggcatagg ttttgcccgt ttcctcacct ttacgatagg ccgggcgcgg
     9361 gtcctgcgcc agtacttcgc ggataaacag cgttaactgc ggataacgct tctccagcgt
     9421 caaaagctgc ttttcgacct ctgcggtaaa actcaccgcc atctctgcag ctggcgcact
     9481 ttgcgcatag ctggcactgg catcgggaag cgattcggca aaggggagat acggtttgat
     9541 atccactacc ggcgtaccat cgaccagatc cagactgccg agcttcagaa tcacgctgtc
     9601 tttatggcaa acaacctctt tcagctctac cagcgacata ccaattgggt tagggcggaa
     9661 agtagagcgt gttgcgaaaa cccccattct ggcgttaccg ccgaggcgcg gtggacgcac
     9721 agtcggacgc cagccgcctt ccatcgtttg atgaaagacg aaaaggatcc ataaatggct
     9781 gaacgcttcc aggccgcgaa cggcgtcggc ctggttgtag ggagcaatga gatgcagttc
     9841 gccgttggcg ctttttacca gacctggctg gcgcggaacg gcgaattttt ctttataggg
     9901 cgagcgaata acgcctattt gctcgaactg gaaactgctc atttcgccgt aatgttaagc
     9961 gcagagccga tacatacagc ctgacgatag cagcctagcg taccgctggt gacttcgcag
    10021 ctatgcagta ataccgcatt ggctttcatt ttagaggcgt tgatttgcat ccgcttacgc
    10081 gcggttggaa tgctcggcgg agagtcctgc ttagaggcct ggcaagagtc gccactgact
    10141 tcaccgagat cgcggaacgg tttgccgact aattcttctg cattggtata aattcggacc
    10201 ggcgtggcgc gcggcgcttt cggttttgca ggctccgctt tcggctgggg tgcagtgctt
    10261 tgaacgggtt cgacagggga tctgcttaac atggaacagc cgcttagcat gagtgctact
    10321 aaacagatcg gtaaagcacg catagtattt cctcaatgta tgatcaaaac gtcaatattg
    10381 aatcaggagc ttgtaaaaat gacaagacgg gcaagcgccc gtcctgaatg atattacaaa
    10441 ttgtggaaac agcctaaaaa ttaccagcct ttaacagcgc cgccgttaaa cactttgttt
    10501 gctgcttcgt aaacttcatc agactgataa gcctggacga atttcttcac gttctcggcg
    10561 tctttgttat cttcacgcgt tacgatcagg ttcacgtacg gggagtcttt atcttcgaca
    10621 aagataccgt ctttcgctgg agtcaggcca atctggctgg cataggtggt attgataact
    10681 gccagagcga tttgcgcgtc gtccagagat cgaggcagct gcggtgcttc cagctcaaca
    10741 attttcagat tttttgggtt ctcaacaaca tcaagaacgg tcggcagcag gccaacgcca
    10801 tctttcagtt tgatcaagcc cactttttgc agcagcagca gtgaacgacc aagattagtt
    10861 ggatcgtttg gcacggcaac ctgcgaacca tcctgcagtt catccagtga tttgattttc
    10921 ttggagtaac cagcaatcgg ataaacaaaa gtgttgccta ctgcgaccag tttgtagcca
    10981 cgatctttca gttgctgatc aaggtacggt ttatgctgga aggcgttggc gtcgatatcg
    11041 cctttgctca atgcttcgtt tggcagaaca tagtcgttga aggttaccag ctcaacgtcc
    11101 aggccatatt tgtctttcgc aactttctgc gcgacttctg ctacctgctg ttcggcacca
    11161 acaatcacgc cgactttaat gtggtttgga tctttttcat cctgaccgca gcctaccagt
    11221 gccagtgatc caatcagggc tcccactgcc gcaaaggttt tgaatttgaa cgccatacct
    11281 tattccttct tcaattattt atgttgtgtt gaacgttact tgcgagtgac agcccggacg
    11341 atgcggtcgc ctgcgaactg aattaaataa accagaatga ccagcaatac cagtaccgta
    11401 ttcatcaccg tcgcgttgta gccgatgtag ccatactgat agccaatctg acctaaacca
    11461 ccggcaccga ctgcaccacc catcgcggaa taaccgacca gggtaatcag ggtgatagtt
    11521 gccgcattca ccagacccgg caacgcttcc ggtaacagca ctttacggac gatctgcatt
    11581 ggcgtggcac ccattgcgcg ggaagcttca attaacccgg ttgggatctc cagcagagcg
    11641 ttctcgacca tacgggcaat aaacggtgct gcaccaacgg ttaacggaac aattgctgcc
    11701 tgcaaaccaa tcgatgtacc gacaataacg cgggtaaacg gaatcatcca taccagcaag
    11761 ataatgaacg ggatggaacg gaaaatgttc acaatcgcag aaacggtacg atacagcttc
    11821 gcgttagcaa taatttgccc cggacgcgtg acataaagca gaacgccaac cggcagacca
    11881 atcacaaagc caaaaaatcc ggatacgaag gtcattgcca gcgtttccca tacgccacga
    11941 accagcagcc acatcatcgg ctcagacata acccagtacc tctactttta catggtgttc
    12001 ctgcagccag gcaatggcgg cttgcgtatc ttgttgtgtg ccgtgcattt cagtcagcat
    12061 gatgccgaat ttaacgccac cggcgtagtc catctgcgcg ctaataatgt tgttgttgac
    12121 gttgaaacga cgcgcggttt cagaaagcag tggggcatcg accgattgac cggtaaactc
    12181 cagacgcagc atcggcacgc agtcagtaaa tggctccgct tgcagacgtt cctggtaatc
    12241 ttccgggata tccagatgca gggtcgactg aataaacttc tgcgccagcg gcgttttcgg
    12301 atgcgagaac acttcactta ccgtgtcctg ctcgatcagt tcgccattgc tgatgaccgc
    12361 cacgcaatca caaatacgct tcacaacgtc catttcgtgg gtgatcaaca gaatcgtcaa
    12421 acccagacgg cggttaatgt ctttcagcag ttcgagaata gaacgtgtcg ttgccgggtc
    12481 cagcgcgctg gtggcttcat cacacagcaa tactttggga ttgctggcta atgcacgggc
    12541 aatcgccaca cgttgtttct gcccaccgga aagattcgac gggtagctat catgcttatc
    12601 gccaagacca accaatgaca gcaattccgt cacgcgacgt ttgatctcgt ctttcggtgt
    12661 gttgtccagc tccagcggca gagccacgtt gccaaaaaca gtacgcgaag agagcaggtt
    12721 aaaatgctgg aaaatcatac caatctggcg gcgagctttg gtcaactcgg attctgacag
    12781 cgtggtcaat tcctggccat cgaccagcac gctaccctcg gttgggcgct ccagcaggtt
    12841 tacacaacgt ataagcgtac tcttacccgc gcctgaggca ccgataacgc cataaatttg
    12901 tccagctggc acatgcaggc tgacgttgtt caacgcctgg atggtgcggg tgccttggtg
    12961 gaacactttg gtgatattcg aaagttttat cattgattat ttattatcgt cattaagtta
    13021 gtcgtggcat ctcgaatgcc tgaaacgggc aacgccgtca atgaaatgga tgttaaggca
    13081 tccagacgtc taaatcaatc aggtttatgc gaagagcact ttcttgcagg tcgaaacatg
    13141 cgatactagc gtcccatgcc ttattaagga gctataaaag gtggcgaaga gcgtacccgc
    13201 aatttttctt gaccgtgatg gcaccattaa tgtcgatcac ggctatgtcc atgagatcga
    13261 caactttgaa tttatcgacg gtgttattga cgccatgcgc gagctaaaaa aaatgggctt
    13321 tgcgctggtg gtagtaacca accagtctgg cattgctcgc ggtaaattta ccgaagcaca
    13381 gtttgaaatg ctgaccgagt ggatggactg gtcgctggcg gaccgagatg tcgatctgga
    13441 tggtatctat tattgcccgc atcatccgca gggtagtgtt gaagagtttc gccaggtctg
    13501 cgattgccgc aaaccacatc cggggatgct tttgtcagca cgcgattatt tgcatattga
    13561 tatggccgct tcttatatgg tgggcgataa attagaagat atgcaggcag cggttgcggc
    13621 gaacgtggga acaaaagtgc tggtgcgtac gggtaaacct attacacctg aagcagaaaa
    13681 cgcggcagat tgggtgttaa atagcctggc agacctgccg caagcgataa aaaagcagca
    13741 aaaaccggcg caatgattaa aagatgagcg gttgaaataa aaatgcattt ttccgcttgt
    13801 cttcctgagc cgactcccta taatgcgcct ccatcgacac ggcggatgtg aatcacttca
    13861 cacaaacagc cggttcggtt gaagagaaaa aatcctgaaa ttcagggttg actctgaaag
    13921 aggaaagcgt aatatacgcc acctcgcgac agtgagctga aagctgcgtc gcaactgctc
    13981 tttaacaatt tatcagacaa tctgtgtggg cactcgaaga tacggattct taacgtcgca
    14041 agacgaaaaa tgaataccaa gtctcaagag tgaacacgta attcattacg aagtttaatt
    14101 ctttgagcat caaactttta aattgaagag tttgatcatg gctcagattg aacgctggcg
    14161 gcaggcctaa cacatgcaag tcgaacggta acaggaaaca gcttgctgtt tcgctgacga
    14221 gtggcggacg ggtgagtaat gtctgggaaa ctgcctgatg gagggggata actactggaa
    14281 acggtagcta ataccgcata atgtcgcaag actaaagagg gggacc
//
LOCUS       DAKISA010000106        13990 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.106, whole genome shotgun sequence.
ACCESSION   DAKISA010000106 DAKISA010000000
VERSION     DAKISA010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13990
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.106"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(8..694)
                     /locus_tag="OVW45_003400"
     CDS             complement(8..694)
                     /locus_tag="OVW45_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCV5527765.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(1275..2063)
                     /gene="phoH"
                     /locus_tag="OVW45_003401"
     CDS             complement(1275..2063)
                     /gene="phoH"
                     /locus_tag="OVW45_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCV5527766.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGGGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(2684..3955)
                     /gene="efeB"
                     /locus_tag="OVW45_003402"
     CDS             complement(2684..3955)
                     /gene="efeB"
                     /locus_tag="OVW45_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCV5527767.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHV
                     SRSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(3961..5088)
                     /gene="efeO"
                     /locus_tag="OVW45_003403"
     CDS             complement(3961..5088)
                     /gene="efeO"
                     /locus_tag="OVW45_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCV5527768.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(5146..5976)
                     /locus_tag="OVW45_003404"
     CDS             complement(5146..5976)
                     /locus_tag="OVW45_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCV5527769.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(6650..8158)
                     /gene="putP"
                     /locus_tag="OVW45_003405"
     CDS             complement(6650..8158)
                     /gene="putP"
                     /locus_tag="OVW45_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HCV5527770.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALLAGAASTILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEN"
     gene            8580..12542
                     /gene="putA"
                     /locus_tag="OVW45_003406"
     CDS             8580..12542
                     /gene="putA"
                     /locus_tag="OVW45_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HCV5527771.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(12582..13220)
                     /gene="rutR"
                     /locus_tag="OVW45_003407"
     CDS             complement(12582..13220)
                     /gene="rutR"
                     /locus_tag="OVW45_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCV5527772.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            13508..>13990
                     /locus_tag="OVW45_003408"
     CDS             13508..>13990
                     /locus_tag="OVW45_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCV5527773.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPVYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     D"
BASE COUNT         3459 a         3817 c         3453 g         3261 t
ORIGIN      
        1 tacttagtta atgcaccggt tcactctttg tcgccgcgac taatgggcga attttgtcac
       61 gagtctccca gaccggagga atttcctcca atttagtgac agaagctcgt acaaaaggca
      121 cccgtgcaag agtcataatt gtatccaacc aacggtcacg aataatgcga tccgcccttt
      181 catgactcgg gtcatccagc tcaataacac agactatttt attgttcttc tttgaaacca
      241 gaacataatc cattgccatg ccattataaa ttgcaaagaa acgctttctt tcttcttcgc
      301 taaactcagg ttgttctgga aagttgatca agcgccccag agagacctgg caatggatat
      361 gacaataatg tccaaaccat ttttccagat tatcgaaata cattttttca caacgcgtga
      421 ttaaccactc ttttctttcc aataaaatac cttcggcaac cattccctga aataactcat
      481 ctcgctcttg ccaacgagag tcacgatagg ttttctgcgg tatatagctc cttgtcgacg
      541 ccttagcggc aggggaagtt gcagatgaag ggacgggttg cgatttagcg atgattaccg
      601 atgaggcaac ttttcggagt gtcaattttc tggcaacagc aaaaccggcg ataaatgcaa
      661 ttgtcaaaca agttacaaaa gccataaagc tcataattaa ttttcaccta tccatgttga
      721 ttatatttca ttaccaatgt tactcgctgc agcgtgttac atgccaacaa tgcaacgatc
      781 aatatatagt tagtatcatg ttttgattat tacaaccatc cctatttagc cattgcggct
      841 tgccttccac aaaaaaataa caacacacaa tattagcaaa gaattaatta agattaacct
      901 tacatttata atattatccc taaagaataa atcagaggaa tatattcggt gttaaacagt
      961 cagtcaaagt atagcccatt gttgattaaa cctcataagc aaaattggca ccagatctcc
     1021 tgtacaggtt gaatggccga aaaaagatag gcagatgctc atttttttgg cttctggaag
     1081 aaaaactctc cgcgctggca gtattacact ggtctgatgc tatgacgaaa aatgcctggt
     1141 aagagtatct aaaccagact ccagttcagc ttatcaacac gctcaaagtc gcccccctga
     1201 acgctaacct gaagtgaaaa tctgttgaaa taaaacaaag cccggttcgc ccgggctctg
     1261 caccgataac acacttaact gtaggcatgc agcgtacgtt ggcaaagtgc cgaacgtacg
     1321 cagtcctctt taccgaaccg gacgatccca accatttcat cttcttcgaa acgttccagc
     1381 gcgtcactta atccggagca cacgccgcga ggcaaatcgc attgcgtgat atcaccgttg
     1441 acgataaccg tcacgttctc cccgaggcgg gttaaaaaca ttttcatttg cgcggcagtc
     1501 acattctgcg cctcgtcaag aatgacgact gcattttcaa aggtacgtcc acgcatatag
     1561 gcgaacggcg caatttccac cttccctatt tccggtcgca ggcagtactg cataaaggaa
     1621 gcccctaagc gccggaccag cacgtcgtag accgggcgaa aatagggagc aaacttttct
     1681 gcgatatctc caggtaagaa gccaagatct tcatcggctt gcagaactgg acgggtgacg
     1741 ataatcctgt cgacatcctt atgtatcagg gcctctgccg cttttgctgc gctgatccag
     1801 gtttttccgc ccccggcttc gcccgtggcg aatatcagct gcttactctc aatagccttc
     1861 agatagtgca attgcgcttc atttcgcgcg aggatgggcg aagtatcgcg actgtcgcgg
     1921 gccataccaa tggcttctac gccgcccatc tgcacaagcg aggtgaccga ttcttcttca
     1981 cgctgcttat ggctgcgcga atcccgtctc agcacacgtt ttgcctcgcg acgagctttg
     2041 atcactgctt tttgtcttcc catagagagc accttgagtt gtttgtattc atcacacgcg
     2101 ccgttggcag cgcgattatg cgcacgaaca tcagagggtt ggcttccttg taagccatag
     2161 tttgcttttg gataaaatgc cgaaaaacgg ctacgcgcac cgtttacggc gtcggtaaca
     2221 catgaaaaga aaggatgagg ttgaaaatgc aaagtgacga gatgactacc ggaggagaaa
     2281 actccgcgag tggtggcgcg ttgattatct aaaacatgtc cagtacagga cgttaccatc
     2341 cgcgatctcc atagtgactg actatcactg ccgggaactt ccgctgctac ttaataagta
     2401 caacagatct cgcatttatt gcaacaatat atttacttat atttaactat aaaacactat
     2461 ttcagtgaca ttagtttcta ctggaaagat gacagagtga tgacagtgat gaaaaaagct
     2521 gtgtgctttc agcaggattt gcatcaggca tcagtgcacg atcgccggat gcggcgtgaa
     2581 cgccttatcc agcctaccaa acccgcactg attgtaggca tgataagacg cgcaagcgtc
     2641 gcatcaggca tctgcgcaca aatgccttat ccgcctaaaa acattaaacc cgcaataacg
     2701 cgcttccgag ataatcgttc gcgtccttca ctcccggcag cgaaaaaaaa taaccgccgc
     2761 cgataggttt aacgtattcc tccagcgctt cgccattgag ccttttttgt actgtcagga
     2821 agcctttttc cagatcgtgt tggtagcaga caaacagcaa ccccatatcc agttgtccgg
     2881 agttggtgac ccccaacgaa taactgtagc cgcgacgcag catcaggctg gactcactct
     2941 ccgccgtgcg gggattcgcc agccggatat ggctgtccag cgcaatcacc ttcccttccg
     3001 ggtcgctggc gtaatcaggc acatcatgct catgctgcat tcccagcggc gcaccggttt
     3061 gcttatcacg gccaaaaatc gtctgctgtt ctttcagcgg cgttctgtcc caaaattcca
     3121 ctcgaaactg aatcaagcgt actgcctgat agctgccacc gattgtccac gcaggctcct
     3181 gctgatctgc cgttacccac accacttttt gcatcaactt atcattctgg ctatcgggat
     3241 tggcagtgcc gtctttgaaa cccagcaaat taatcggcgt ctctttgcct ttactacgcg
     3301 acacgtgatc ggaaataaac ccttcccgct tccagcgcac actgagcaaa tccggcgtgt
     3361 gtttgatgat atcgcgcagc gcatggataa ccgtgtcctg ggtgttggcg caaatctgta
     3421 gcaacacatc accatgacat aacgccgcat ccagcgagtc gttggggaaa cgcgtcatct
     3481 tctgcagctt ttttggcatc tgtggcgcaa ggccaaagcg ctcatcaaac aatgagtgac
     3541 ccaccgataa cgtgatggtg agattatcgg gcgcaatgta gccgccaaga atgccggaat
     3601 cgagtggtgg caggcgcgga tttggcgttt ctggtgctgc tccgccctga gtcagaaaag
     3661 caaaacgctg agtcaacaag cgaaacaacc gctcaagatc ggctttatcg ctggcaagca
     3721 catcaaacgc caccagcatc attgcggcct gttgtggcgt caggatccct gcctgatgct
     3781 caccataaaa cggctgtttc tcattgcgtg catccggtga aagcgtgccc ggtgcacttt
     3841 gcgttttttg tgcatgagcg accggacaac ttcccgccag cgccagcgca ccaatccctt
     3901 tcagtaaacg tcggcgtgac ggttcattca cgccgttttc atctttatac tgcataacgc
     3961 ttaatccagt cccagcacac cgcgaagttg cgccagatct tccgccagcg cagtaatcgg
     4021 tcctttcagt gcattccggt cggcatcggt caatttgtcg taggtttcaa aaccgtcttt
     4081 agtacggtat ttcgccagaa tggtatcgac ctttttaaag ttggcatcga cttttgccag
     4141 cagttccggg ttggcttttt gcagttgtgg acgcagcaaa tcgacaattt tctgcgagcc
     4201 ttcaacgtta gcctggaaat cccacagatc ggtgtggctg tagcgatctt cttcaccgct
     4261 aattttgctg gctgccactt cctcaatcag tccggctgcg ccgccgacca cttttgaagg
     4321 tgggaaagcc agttcactga tgcgtttttg caaatcgacc acatctgtat aaagctgctc
     4381 agcgtactga tccatccctt tggtggtgtt gtcgccaaac aatgcttttt ccagacggtg
     4441 gaaaccagtg aattttgggt cggcggcttt ttgctcgtaa tcatcttcac gggcgtcaat
     4501 gctgccatcc agatcggaga acagttcagc aatcggttca atgcgctcat agtgctggcg
     4561 cgtcggcgca tacagtgctt tcgctttttc gatatcgcct gctttaatcg cgtcggtaaa
     4621 ggctttggtg tcggtcacca gctgcgtggt ttccgccatg acatacgctt tatatgcagt
     4681 aattgcacca ccaagactta acagcgcatc actttgcgcc gcatccgccg ttgcctcacc
     4741 tttgacgatc aacttccctt tcgggttagt cagcagaccg caggtcatat cgtattcgcc
     4801 aggctgtaaa ttcgccgtca ttttctggct aaaaccaggg gcgatatttt cccgctcttc
     4861 caccaccatc acgcctttga ggatctccca ctccagcgcc ttctggctat ggttctgaat
     4921 aatgaactgt gttttcccgg cgttaaccgt aatggtcatc ggttcgcact gcttatccgt
     4981 cacggtcact ttgacctgcg gcacatcagc ggcgttagcc ataaaagcag aagaaaacag
     5041 cgcagccacg ctcaactgca atgcgttacg gcggaagtta atggtcatga cgagactatc
     5101 cctttaaagt atgttgtaac taagcaagag tttgcgtcgt atttgttacg cggagcgaga
     5161 cgctgtcgcc cctgcgcgtg gtggcagagc aaatgccacc agcgccggga tgagataaat
     5221 aaaccagacg gcgacttcgc tgacgctcgg tgcttcctga tagccaaaaa tcccttccat
     5281 cagcgtgcca aacagcgagt gagttgagag cactgcgctc atatcgaagg cgatttcctg
     5341 aaagtggttc cacaatccgg cttcatgaaa tgcgcgaatg gcaccagcag ccagccctgc
     5401 ggcgacgaag agaataaaca ggctggtcca tttaaaaaat gcaccaagat tgaggcgaat
     5461 accgccccag tagagcagga agcctagcac cacggcagta gcaagaccga gcattgcacc
     5521 cagcggcggc cagatcccga catcttgttg aaatgccgcc agcaggaaaa agaccgactc
     5581 cagcccttcc cttgcaacgg caaaaaagac catcatcacc agcgcccagc catgatgatt
     5641 tccacgctgc aatgcgctat cgactgcctg ctccagttgt actttgacgt tgcgcgacac
     5701 tttgcgcatc cagaaaacca tccaggtaag gatcaccacg gcgatcaccg ccacgatacc
     5761 ttcaaacagt tcctgttctt tttgcggaaa ttcgccggtg gtttcgttaa tgaagatacc
     5821 caagcccagg cacaacgcag cggcaagcaa cacgccaatc cacatcacgc caatccatcg
     5881 gcctcgctgg gtacgcttaa gatagctggc aatcaaactg acaatcagcg cggcttcaag
     5941 tccttcgcgc aacataatga gaaacggaac aaacatgcca cccaccctta aacgtttttc
     6001 tgagtcaatc tatgcaaaga aaagtaaatc gcagtgatag cgattatcat tacggcaaag
     6061 aaaaatacaa gcggaatgtt tataaaatga tgttggaatg taaacaaaga gaggtaatac
     6121 caggggatat aaacgattat tccaggtaca aagatgggat tgtttacaac gagccatgtg
     6181 ttcttgccag atgccgcgta aacgccttat ccagccgaca aaactgtcca gactcaatgc
     6241 atcacagctt ccatgtaaaa ctgataagcg ggtgtatcaa gcaatttcgt ccatccgcga
     6301 ttatcaaatc taactttttg cccggaacat aataacagcg cgggtgttgg tggcgtcaaa
     6361 atcgctgaaa cgttccccga aggccggggc agcccacatg gatgtgggct gagggcgcgt
     6421 cttacaggac gttacctcgc gcccgacccg gtagccgaag gggataagct gaaggtaccg
     6481 cgcagcggcg attttgttcg ccagagcccg ggggtgcagg gggcggcggc gactggccgc
     6541 cccctgcgcg ctccctgcac cattcaattt atatggttat atggcgaggc acattaaggg
     6601 agcgcaaccc gctgctgaag ccaaacaaaa ccgccgcagg ctaagtccct tagttttcct
     6661 gcaaccgtga cggcggagcc gaatgatagt gcgcatcggc ctcggcaaag cgtttttgca
     6721 tcgccgctga cggcgcttta cccagcaaac taaacactac aatcccgata ctgccgaaga
     6781 tgaagcccgg aataatttcg tacagtccca gccagccgaa ctgcttccag acgataactg
     6841 tcagcgcacc gatgatcatc cccgccagcg caccgttgcg cgtcatgcgt gaccacatca
     6901 ccgagaacag caccaccgga ccaaacgccg cgccaaagcc tgcccacgcg tagctcacta
     6961 agcccagcac gcggttttcc gggtttgccg ccagcgcaat cgccaccagc gccaccacca
     7021 gcaccatcac acgtcctacc cacaccagct ctttctggct ggcctgttta cgcagaaacg
     7081 ctttgtataa atcttcggta atcgcactgg agcacaccag cagctggcaa cttaaggttg
     7141 acattaccgc cgccagaatc gccgacagca gaatcccggc aatccacggg ttaaacagaa
     7201 tttgcgccag ttcgataaac acgcgttcag cgttctggtt taccgcacct gctaccgccg
     7261 gatgctcgtt aaagtaagcg ataccaaaga agccaacagc caccgcccct gccaggcaaa
     7321 ggatcatcca ggtcatactg atacgacgcg catggacaat gctgtggtga gaatctgccg
     7381 ccataaaacg cgccaggatg tgcggctgcc cgaagtaacc cagcccccaa cccatcaatg
     7441 agataatggc aacaaagttc agccctttga gcatatccac gttttcgatg ctcttttgtt
     7501 tgatcacttc cagcgagtca ccaaagccac cgacactgat aatgacgata accggcgtca
     7561 ggatcagggc aaaaatcatc aggctggcct gtacagtgtc agtccagctc accgcgagga
     7621 aaccgccaat aaaggtgtaa aggatcgtcg acgcagctcc cgccaacaga gccgtttcgt
     7681 aactcatgcc aaaggtactt tcaaacagac gcgcgcctgc cacaatgccc gaagcgcaat
     7741 aaatggtgaa gaacagcaaa ataaccagcg cggagataat gcgcaaaatg cggcttttat
     7801 cttcaaaacg cccggtgaaa taatccggca atgttaaggc gttattattg tattcggtat
     7861 gcacacgcaa ccgcccggcc accagcttcc agttgatcca cgcgcctaat gtcaggccaa
     7921 tggcgatcca gctttcggaa atcccggaaa gaaaaaccgc gccaggcaac cccattaaca
     7981 gccagccgct catatccgac gcacccgccg ataatgccgt cacgaatggc ccaagactac
     8041 gaccgcccag aatatagtcg tcaaagtttt tcgttgatcg ccaggcgata aacccaatca
     8101 atatcatgcc aaagatatag acacaaaatg tcaccaacat cggtgtgcta atagccatct
     8161 aaagtctcca aaaaattatt atcggcaatg tcgaaacttg ccgttatatc tgccaccgga
     8221 acggggtaac agagtttatg ttttaccagg gcgaccgtat cctgccggaa gcgctggtta
     8281 ttcacaatcg atttaacaca ccatttacat taaattttag tgctcagcga cactattttt
     8341 catcaggttg cactctctca cattttttgc ggttgcacct ttcaaaaagg ttaactgccg
     8401 cagagaaaaa gtctgagtta ttttttaatc actgtcatat cgatttcttt tattaacatt
     8461 tcattcattt ttaagcttgc tacgcaagtc acatttaaca tggttgcaca aagttgcaac
     8521 atcatggata tttcacgata acgttaagtt gcacctttca gaacaacagg agtaatggca
     8581 tgggaaccac caccatgggg gttaagctgg acgacgcgac gcgtgagcgt attaagtctg
     8641 ccgcgacacg tatcgatcgc acaccacact ggttaattaa gcaggcgatt ttttcttatc
     8701 tcgaacaact ggaaaacagc gatactctgc cggagctacc tgcgctgctt tctggcgcgg
     8761 ccaatgagag cgatgaagca ccgactccgg cagaggaacc acaccagcca ttcctcgact
     8821 ttgccgagca aatattgccc cagtcggttt cccgcgccgc gatcaccgcg gcctatcgcc
     8881 gcccggaaac cgaagcggtt tctatgctgc tggaacaagc ccgcctgccg cagccagttg
     8941 ctgaacaggc gcacaaactg gcgtatcaac tggccgataa actgcgtaat caaaaaaatg
     9001 ccagtggtcg tgcaggaatg gtccaggggt tattgcagga gttttcgctg tcatcgcagg
     9061 aaggcgtggc gctgatgtgt ctggcagaag cgttgttgcg tattcccgac aaagccaccc
     9121 gcgacgcgtt aattcgcgac aaaatcagca acggtaactg gcagtcacac attggtcgta
     9181 gcccgtcact gtttgttaat gccgctacct gggggctgct gtttactggc aaactggttt
     9241 ccacccataa cgaagccagc ctctcccgct cgctgaaccg cattatcggt aaaagcggtg
     9301 aaccgctgat ccgcaaaggt gtggatatgg cgatgcgcct gatgggcgag cagttcgtca
     9361 cgggtgaaac catcgcggaa gcgttagcca atgcccgcaa gctggaagag aaaggtttcc
     9421 gttactctta cgatatgctg ggcgaagctg cactgactgc cgcagacgca caggcgtata
     9481 tggtttccta tcagcaggcg attcacgcca ttggtaaagc gtctaacggt cgtggcatct
     9541 atgaaggtcc gggcatttca atcaaactgt cggcgctgca tccgcgctac agccgcgccc
     9601 agtatgaccg ggtaatggaa gagctttacc cgcgtctgaa atcactcacc ctgctggcgc
     9661 gtcagtatga tattggtatt aacatcgatg ccgaagaggc cgatcgtctg gagatctccc
     9721 tcgatctgct ggaaaatctc tgtttcgagc cggaactggc aggctggaac ggcatcggtt
     9781 ttgttattca ggcttatcaa aaacgctgcc cgttggtgat cgattacctg gttgatctcg
     9841 ccacccgcag ccgtcgccgt ctgatgattc gcctggtgaa aggcgcgtac tgggatagtg
     9901 aaatcaagcg tgcgcagatg gacggccttg aaggctatcc ggtttatacc cgcaaggtgt
     9961 ataccgacgt ttcttatctc gcctgtgcga aaaagctgct ggcggtgccg aatctaatct
    10021 acccgcagtt cgcgacgcac aacgcccata cgctggcagc gatttatcaa ctggcaggtc
    10081 agaactacta tccgggtcag tacgagttcc agtgcctgca tggtatgggc gagccactgt
    10141 acgagcaggt caccgggaaa gttgccgacg gtaaacttaa ccgaccgtgt cgtatttatg
    10201 ccccggttgg cacacatgaa acgctgctgg cgtatctggt gcgtcgcctg ctggaaaacg
    10261 gtgctaacac ctcgtttgtt aaccgtattg ccgacacctc tttgccactg gatgaactgg
    10321 tcgccgatcc ggtcactgct gtagaaaaac tggcgcaaca ggaagggcaa actggattac
    10381 cgcatccgaa aattcccctg ccgcgcgatc tttacggtca cgggcgcgac aactcggcag
    10441 ggctggatct cgctaacgaa caccgtctgg cctcgctctc ctctgccctg ctcaatagtg
    10501 cactgcaaaa atggcaggcc ttgccaatgc tggaacaacc ggtagcggca ggtgagatgt
    10561 cgcccgttat taaccctgcg gaaccgaaag atattgtggg ctttgtgcgt gaagccacgc
    10621 cacgtgaagt agaacaggcg ctggaaagtg cggttaataa cgcgccaatc tggtttgcca
    10681 cgcctccggc tgaacgcgcg gcgattttgc atcgcgctgc cgtgctaatg gaaagccaga
    10741 tgcagcaact gattggtatt ctggtgcgtg aagccggaaa aaccttcagt aacgccattg
    10801 ccgaagtgcg cgaagcggtc gattttctcc actactacgc cggacaggtg cgggatgatt
    10861 tcgctaacga aacccaccgt ccattagggc ctgtggtgtg tatcagtccg tggaacttcc
    10921 cgctggctat tttcaccggg cagatcgctg ccgcactggc ggcaggtaac agcgtgctgg
    10981 caaaaccggc agaacaaacg ccgctgattg ccgcgcaagg gatcgccatt ttgctggaag
    11041 cgggtgtacc ccctggcgtg gtgcaattgc tgccaggtca gggggaaacc gtgggcgcgc
    11101 aactgacggg tgatgatcgc gtgcgcgggg tgatgtttac cggttcaacc gaagtcgcta
    11161 cgttactgca gcgcaatatc gccagccgcc tggacgctca gggtcgccct attccgctca
    11221 tcgctgaaac cggcggcatg aacgcgatga ttgtcgattc ttcagcactg accgaacaag
    11281 tcgtcgtgga tgtgctggcc tcggcgttcg acagtgcggg tcagcgttgt tcggcactgc
    11341 gcgtgctgtg cctgcaagat gagattgccg accacacgtt gaaaatgctg cgcggtgcaa
    11401 tggccgaatg ccggatgggt aatccgggtc gcctgaccac cgatatcggt ccggtgattg
    11461 atagcgaagc gaaagccaat attgagcgcc atattcagac catgcgtagc aaaggccgtc
    11521 cggtgttcca ggcggtgcgg gaaaacagcg aagatgcccg tgaatggcaa agcggcacct
    11581 ttgtcgcccc gacgctgatc gaactggatg actttgccga attgcacaaa gaggtctttg
    11641 gtccggtgct gcatgtggtg cgttacaacc gtaaccagct accagagctg atcgagcaga
    11701 ttaacgcttc cggctatggt ctgacgcttg gtgtccatac gcgtattgat gaaaccatcg
    11761 cccaggtcac tggctcggcc catgttggta acctgtatgt taaccgtaat atggtgggcg
    11821 cagtggttgg tgtgcagccg ttcggcggcg aagggttgtc cggtaccggg ccgaaagcag
    11881 gcggtccgct ctatctctac cgtctgctgg cgaatcgccc ggaaagtgcg ctggcagtga
    11941 cgctcgcgcg tcaggatgca gagtatccgg tcgatgcaca gttgaaagcc gcattgactc
    12001 agccgctaaa tgcactgcgg gaatgggcag caaatcgtcc agaattgcag gcgttatgta
    12061 cgcaatatgg cgagctggcg caggcaggaa cacaacgatt gctgccgggg ccgacgggtg
    12121 aacgcaacac ctggacgctg ctgccgcgtg agcgcgtgtt gtgtattgcc gatgatgagc
    12181 aggatgcgct gactcagctc gccgccgtgc tggcggtggg cagccaggta ctgtggccgg
    12241 atgacgcgct gcatcgtcag ctagtaaagg cattgccatc ggcagtcagc gaacgtattc
    12301 aactggcgaa agcggaaaat ataaccgctc aaccctttga tgcggtgatc ttccacggtg
    12361 attcggatca gcttcgcgca ttgtgtgaag ccgttgctgc gcgggatggc gcaattgttt
    12421 cggtgcaggg ttttgcccgt ggcgaaagca atatccttct ggaacggctg tatatcgaac
    12481 gttcgctgag tgtgaatacc gctgccgctg gcggtaacgc cagtttaatg acgatcggtt
    12541 aaacaggtag ccggaggatg ttacaacctc ctccggcatc tttaacgtgg tcgaatcccc
    12601 tcaataataa tccgctgcac gttttcaacc gtttgattga aaaatatctc atcgcgcaac
    12661 gtcgcgcctg tcaccgcctc cacctgaggg gcgaaatcgg cgtaatgttg agtggaagcc
    12721 caaatcatga aaatcaaatg ctgcggatca atcggcgcga gtttgccgct tttgacccaa
    12781 ccggcaatca gcgcagattt ctcatcaatt aatgccttca aatcgcccgt cagttcatcc
    12841 attaacagcg gcgcgcctgc cagcatctcc atacagaaca ggcgcgaagc ctgcggataa
    12901 tcgcgggaga cttccagctt cagacggatg tactctttta tcgccgccag cggagcgaaa
    12961 tcttcacgaa acgcttttaa cggtgccagc cagatatcga gaatctgccg cagcacggca
    13021 atatacagcg cctcttttga cggaaagtaa tacagcagat tggtttttga aacacccgcc
    13081 aactctgcga tctgctccag ccttgtgccg tgaaaaccga attgtgaaaa agtgtccagt
    13141 gctgcgctaa gaatcgcttt tttcttcgcg cttactgcgc gcgaacgttt acccgttgtt
    13201 ttcactgcgc cttgcgtcat gcgctctccc ctcttgtaga ttggcaacag gatagcaaaa
    13261 cccccgcccg gcctcgacct tctgcactct catcgcgcac agatgcatgt tttatgtgca
    13321 actgttttga ccgtttagtc cactttttat cagatatttt agataacgta atattaacat
    13381 attaataaca ttaacttttt aaaaactggc atccgctttg caaacaagcc aatacgcggc
    13441 tgaaaaggat atgcaggatg cagcgccccg tctgactttc actttacgag acgaagagag
    13501 gttgatgatg aaaattggcg tattcgtacc tattggcaac aacggctggc tcatttcgac
    13561 ccacgcgccg cagtacatgc cgacctttga actgaataaa gccattgtgc agaaagcgga
    13621 gcattaccat ttcgatttcg ccctgtcgat gatcaaactg cgtggctttg gcggcaaaac
    13681 tgagttctgg gatcacaacc ttgagtcgtt caccttgatg gcgggtctgg cagccgtgac
    13741 ctcgcgcatt cagatttacg ccactgctgc caccttaacg ttacctccgg caatcgtcgc
    13801 ccgtatggcc gcaaccatcg actccatctc tggcgggcgt tttggcgtca acctcgtgac
    13861 tggctggcaa aagcccgtgt atgagcagat gggtatctgg cctggcgatg actatttctc
    13921 ccgtcgttac gactatctca ccgaatatgt tcaggtgctg cgcgacctgt ggggcacggg
    13981 gaaaagcgat
//
LOCUS       DAKISA010000107        13926 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.107, whole genome shotgun sequence.
ACCESSION   DAKISA010000107 DAKISA010000000
VERSION     DAKISA010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13926
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.107"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..890)
                     /gene="aldA"
                     /locus_tag="OVW45_003409"
     CDS             complement(<1..890)
                     /gene="aldA"
                     /locus_tag="OVW45_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCV5527774.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIIDS
                     RVINSGQVCNCAERVYVQKG"
     gene            complement(1087..1218)
                     /locus_tag="OVW45_003410"
                     /pseudo
     CDS             complement(1087..1218)
                     /locus_tag="OVW45_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
     gene            1219..1764
                     /locus_tag="OVW45_003411"
                     /pseudo
     CDS             1219..1764
                     /locus_tag="OVW45_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            complement(1806..2807)
                     /gene="gap"
                     /locus_tag="OVW45_003412"
     CDS             complement(1806..2807)
                     /gene="gap"
                     /locus_tag="OVW45_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCV5527775.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            2996..3526
                     /gene="cybB"
                     /locus_tag="OVW45_003413"
     CDS             2996..3526
                     /gene="cybB"
                     /locus_tag="OVW45_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCV5527776.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVMYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            3771..3944
                     /locus_tag="OVW45_003414"
     CDS             3771..3944
                     /locus_tag="OVW45_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5527777.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(4016..4165)
                     /gene="hokB"
                     /locus_tag="OVW45_003415"
     CDS             complement(4016..4165)
                     /gene="hokB"
                     /locus_tag="OVW45_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HCV5527778.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            4564..6204
                     /gene="trg"
                     /locus_tag="OVW45_003416"
     CDS             4564..6204
                     /gene="trg"
                     /locus_tag="OVW45_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCV5527779.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASCIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLNRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(6242..7165)
                     /locus_tag="OVW45_003417"
     CDS             complement(6242..7165)
                     /locus_tag="OVW45_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCV5527780.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            7382..8725
                     /locus_tag="OVW45_003418"
     CDS             7382..8725
                     /locus_tag="OVW45_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCV5527781.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            8950..10605
                     /gene="opgD"
                     /locus_tag="OVW45_003419"
                     /gene_synonym="mdoD"
     CDS             8950..10605
                     /gene="opgD"
                     /locus_tag="OVW45_003419"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCV5527782.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            10745..10969
                     /locus_tag="OVW45_003420"
     CDS             10745..10969
                     /locus_tag="OVW45_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCV5527783.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            11032..11568
                     /gene="rimL"
                     /locus_tag="OVW45_003421"
     CDS             11032..11568
                     /gene="rimL"
                     /locus_tag="OVW45_003421"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCV5527784.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(11563..12543)
                     /locus_tag="OVW45_003422"
     CDS             complement(11563..12543)
                     /locus_tag="OVW45_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HCV5527785.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLWQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIRDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            12667..13659
                     /gene="tehA"
                     /locus_tag="OVW45_003423"
     CDS             12667..13659
                     /gene="tehA"
                     /locus_tag="OVW45_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCV5527786.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            13656..>13926
                     /locus_tag="OVW45_003424"
     CDS             13656..>13926
                     /locus_tag="OVW45_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCV5527787.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNL"
BASE COUNT         3546 a         3277 c         3519 g         3584 t
ORIGIN      
        1 atgcctttct gtacataaac acgttctgca cagttacaca cttgcccact attaatgacg
       61 cgtgaatcaa tgatggcttt gactgccagt tcaagatcgg catcgtccat tacgatagct
      121 ggtgctttac cacccaattc cagacacact ttggtgatgt ttttcgccgc agtcgccatg
      181 atcttctcac ctgcagagac gctgcctgtc atactgacca ttgcgacctt tgggttaccc
      241 gccagttctt gcccaacggt ttcaccacgc cccagtacaa ggttaaacac gccgcgcgga
      301 aggcctattt catcgacgat tttggcgaat gcaatcgcat tgtttggcgt aaattcactg
      361 ggtttaatga cgatggtatt accggtcaaa agagcgggag ccattttgcg ggcaatgagg
      421 aagaacggga agttccacgg cagaatgccg gtagtcacac caagcgcacg tttaaacaga
      481 agaatatttt ctcctggacg atcgctttga ataatctcgc cctcgtaacg ccgtgcccac
      541 tccgccatgt aatcgatata gtcggcagta aaagccactt cgacttcagc cagctgctgg
      601 atcttgcccc cttcttcaac aatcagcgca ctgatttcac tggcgcgttc gcggatcccg
      661 gcggagattt tgcgcaacca actggcgcgt tcaatagcag gcaacgcttc ccattctggt
      721 tgtgcacgtt ctgctgcatc gattgcctta cgggcatcct cggcctgacc atcgggtata
      781 cgggaaatga cagcctctgt agcagggttt accacatcaa tccatgcgtc tccacgccag
      841 gtaacaaact gtccatcgat atacatagga tgttgaacgg gtactgacat gggcgactcc
      901 tgtgatttat atgtttgttt tcggtgaata tattgttaat taacatcaat catcagagag
      961 acggcatcgg gaaaggacaa ttctgtgaag ggcttcataa aaacaagtgt aaaaggaata
     1021 aaaggcagaa ataaaagtca ggagtcatgc ccgtttgcaa acaagtggtt cacaaacggg
     1081 caatttttaa cgtaacgagc gactttccat cgcctcgatg agcaccgcat catgtttcag
     1141 cgtttgccat gcatggatga cttctgccgg aaaatcaacg tgaacgataa aatctcgccc
     1201 gcggggaccg tagccattga tcagttcgtc aatcggctgg gtgaagcgat gcaggcggtt
     1261 aaatttggta accccgctga acgcaacgac attgcgatgg ggccgttgat taacgccgcg
     1321 gcgctggaaa gggttgagca aaaagtggcg cgcgcagtag aagaaggggc gagagtggcg
     1381 ttgggtggca aagcggtaga ggggaaagga tattattatc cgccgacatt gctgctggat
     1441 gttcgccagg aaatgtcgat tatgcatgag gaaacctttg gcccggttct gccggtggtc
     1501 gcatttgaca cgctggaaga ggctatctca atggctaatg acagtgatta cggcctgacc
     1561 tcatcaatct atacccaaaa tctgaacgtc gcgatgaaag ccattaaagg gctgaagttt
     1621 ggtgaaactt acatcaaccg tgaaaacttc gaagctatgc aaggcttcca cgccggatgg
     1681 cgtaaatccg gtattggcgg cgcagatggt aaacatggcc tgcatgaata tctgcagacc
     1741 caggtggttt atttacagtc gtaatgagta aaagaggcgg aggttttttc ctccgcctgt
     1801 gcgcgtcaga gtttagcgaa tttttcgagg gtgcgaataa gctgcgtgac gaagccatat
     1861 tcgttatcgt accaggcgac cgttttcacc agttgtaaat cgcccacggc ggtaatttcc
     1921 gtttgcgtgg catcaaacac cgaaccgaaa tggctgccaa tgatatcgga agagactatt
     1981 tcttcatcgg tataaccaaa tgactcgtta ttggtggttg cttgtttaag tgcgttattc
     2041 acctcttcgg cagtcacttt ttttccgaga atcgatacca gttcagtgac cgaacctgtt
     2101 ttcaccggca cgcgttgcgc atgacctttc agtttgccgc tcagttccgg gatcaccaga
     2161 ccaatggctt ttgccgcccc cgtagtgtgg ggaatgatat tttctgccgc tgcgcgtgaa
     2221 gcacgtaaat ctttaccacg tgggccatcc accagtgact gggtgccagt ataggcatga
     2281 atggtcgtca tcgtgccgac ttctatcccg aaactgtcat gcaaggcttt ggccatcggc
     2341 gcaagacagt tagtggtgca tgacgccacg gaaacaatgg tgtcgttgcc atccagagtg
     2401 tcgtcattga cgttataaac gatagttttc atttcaccgg caggggcgga aatcaacacc
     2461 ttcttcgcac cagcatcaag atgtgcctgc gatttctcgg cggaggtata aaagccagta
     2521 cattcgacaa tgatttctgc acctttcgct ttccacggaa tatttttagc ctctttttcg
     2581 gcgtaaaccg cgatactttt cccatcaacg ataagtgaat cttccgtaaa atcaacgctc
     2641 caggggaatg gtccgtagtt tgaatcatgt ttcagcaggt aggcgagaat ttttggggaa
     2701 gtgagatcat taatagcgac aacgtctatg ttgcttttga cttcaagtaa tcgacgcaac
     2761 accagtcgac cgatacgacc aaaaccgtta ataccaactt tactcatggt tttctcctgt
     2821 caggaacatt cggatgaaaa ttgatccttt ccaggcttag accaggattg tgggatgggc
     2881 aatctccatt cttacagtga aacgtaacgt actgaaaacg ggtgaacaat atttaatgaa
     2941 attttgagaa aagcccgtta tgttaacgga aaattatgtt aaagcaggaa atgttatgga
     3001 aaataaatac tcaaggttac aaatcagcat tcactggctg gtctttttgc tggttatcgc
     3061 agcgtattgc gcaatggagt ttcgtggttt cttcccacgt agcgatcggc cactcatcaa
     3121 catgattcat gtttcctgcg gcatctcaat tctcgtgctg atggtcgttc gtcttttgtt
     3181 aaggctgaaa tacccaaccc cgccgattat acctaagcca aaaccgatga tgacgggact
     3241 ggcgcatttg ggacatttgg tgatgtatct cctgtttatt gcgctgccag tgattggttt
     3301 ggtgatgatg tataaccggg gcaacccgtg gtttgcgttt ggtttgacga tgccttacgc
     3361 ttcagaggcc aatttcgaac gggttgatag cttaaagtcg tggcatgaga cgctggcgaa
     3421 tctgggatat tttgtcatcg ggttgcacgc tgcggctgca ctggcacacc actatttctg
     3481 gaaggacaac acacttctac gcatgatgcc gcgtaaacgt tcctgaagga tatttcaaga
     3541 aaatgcctgt actaaaaccg acccgtggta caggcgaaga atacgggtct acatcggaag
     3601 cgcctattat atttatttgt atgacaaata aaaccataat ccttgcccat acgtccatct
     3661 ggcttatttt taatcaattc acccgatctt tgatctcatc aacggtatca aaataaacca
     3721 gcgtataacg ttcaaatcga acataagatt cgataaacca tggaggttat atgaaaaaac
     3781 tggcacttat tttgtttatg ggaacgcttg tttcctttta tgccgatgcc gggcgcaaac
     3841 cctgttctgg ttcgaaaggg gggatctcac actgtacggc aggcggcaaa tttgtctgta
     3901 atgatggttc tattagtgca tcgaaaaaaa catgcactaa ctgaagtgta aaaggggtgc
     3961 catgagaaaa tgaatctgct agtcagatgc gcggggaaaa tccccgcgct tgcccttacc
     4021 tggacgtgca ggccatgagc gcagcaacct ccttatcacc gtcccggaac cgcagttcgt
     4081 agagcgtttg tcgggtcagg agtgtgaatg tcagaatcgt aatgcagata atgagcagac
     4141 acactaccag agggttgtgc ttcatagcct ttctccttgc cggatggcgg gtaagaggct
     4201 aagatctgaa ttgctaggtt cattcgttgg cctcggttga tagaaatatc ggtcggggcc
     4261 ttcgtctttc tgattcccgg ttagcctgaa aacagaaagt ctcaggcacc cgcaggcatc
     4321 ctatgaggtt tccttaggga cgaaaataat cacttcacga aattgcgtgc tgttttccag
     4381 aatttttcgt cattcgggtt agccagttta gccattcgtt actctcttca ttccaatagc
     4441 attaattttc tctgcaataa ttgttgtaaa aatgtgacgc aaagaggttt ttggtcataa
     4501 gtaattaccg tcaagtgccg atgactttct atcaggagta aacctggacg agagacaacg
     4561 gtaatgaata caactccctc acagcgatta ggttttttgc atcacatcag gctggttccg
     4621 ttatttgcct gcattcttgg cggtatctta gttctattcg cattaagttc tgctctggct
     4681 ggctatttcc tctggcaggc cgatcgcgat cagcgtgatg ttactgcgga gattgagatt
     4741 cggaccgggt tagcgaacag ttcagatttt ttgcgttcag cccggatcaa tatgattcag
     4801 gccggggctg cgagttgtat tgcggaaatg gaagcaatga agcgaaatat tgcgcaagcc
     4861 gaatcggaga ttaaacagtc gcagcaaggg tatcgtgctt atcagaatcg gccggtgaaa
     4921 acacctgctg atgaagccct cgacactgaa ttaaatcaac gctttcaggc ttatatcacg
     4981 ggtatgcaac ctatgttgaa atatgccaaa aatggcatgt ttgaagcgat tatcaatcat
     5041 gaaagtgagc agatccgacc gctggataat gcttataccg atattttgaa caaagccgtt
     5101 aagatacgta gcaccagagc caaccaactg gcggaactgg cccatcagcg cacccgcttg
     5161 ggtgggatgt tcatgattgg cgcgtttgtg cttgccctgg tcatgacgct gataacattt
     5221 atggtgctac gtcggatcgt cattcgtcca ctgcaacatg ccgcacaacg gattgaaaaa
     5281 atcgctagtg gcgatctgac gatgaaggat gaaccggcgg gtcgtaatga aatcggtcgc
     5341 ttaaatcgtc atttacagca aatgcagcat tcactgggga tgacagtagg gactgttcga
     5401 cagggtgcgg aagagattta tcgtggcacc agcgaaattt cagctggcaa tgcggacctg
     5461 tcatctcgca ccgaagaaca agcggcggct atcgaacaaa ctgccgccag catggagcaa
     5521 ctcactgcga cggtgaaaca gaatgcggat aacgcgcatc atgccagcaa actggcgcaa
     5581 gaggcttcta ttaaagccag cgatggcggg cagacggttt ccggtgtagt aaaaacgatg
     5641 ggcgctatct ccacgagttc gaagaaaatt tctgagatca ccgccgtcat caacagtatt
     5701 gctttccaga cgaatattct ggcactgaat gctgccgttg aagccgcgcg agcgggtgag
     5761 caagggcgtg gatttgccgt tgtcgccagc gaagtacgga cactcgcaag tcgcagcgct
     5821 caggcggcga aagagattga aggcttgatc agtgaatcag tcaggttaat tgacctgggg
     5881 tcggatgagg tggcaacggc cgggaaaacc atgagcacta ttgttgatgc cgtcgcgagt
     5941 gtcacacata tcatgcagga aatcgccgcc gcctcggatg aacaaagtag aggcataacg
     6001 caggttagcc aggcgatttc tgaaatggat aaggtgacgc aacagaatgc ttctctggta
     6061 gaagaggcct cagcggcggc ggtgtccctt gaagaacagg cggcacgatt aactgaggcg
     6121 gtggatgtat tccgtctgca caaacattct gtgtcggcag aacctcgcgg agcgggtgaa
     6181 ccagttagtt tcgctacggt gtgaaaatgt tcaaggaggg atcgacagat cccttcacct
     6241 ttcagaacgg cattgatttt cgaatagcgt taatcatcaa ctggcaacca gaagagaacg
     6301 tcgcatctac gcgggtcagt attccaatcg gttcgcctgc accatgtccc ggaacaggca
     6361 gggccaccag cgtggcatga cgcaggtcgt cttttacagc gccagaaggg acaaaccaca
     6421 cgtaatcgta ttcaaccgta agttgacgag atagcgaagc agacagcgtt tcgatacaac
     6481 ccgaaggaat tttacagccc tggctctgca ctaatgcatc tgaatgctgg cgtggcgcag
     6541 tgccttctgg tgatacaacg accggccatt ccagcacccg gcttagcgtt acgttctcct
     6601 gaagtagcgg gtgattaggg cggacaacca gcttcagcga ttcaagaaac agcagttcgt
     6661 aattaagccc ggtcatcagt tcaggatctg acatccgacc aatgccgata tcgatttccc
     6721 cggtttttaa acccgccaga atcatagggt tactcattgt cgcaacttgc aaggtcgtct
     6781 ctttttgttg ctgatgaaac tgacctataa ccgaaggtaa tatccccagt gccgcagtag
     6841 gtagtgcacc aaccctgacg acatcattat taagaccttc tttacgatga agcgactgtc
     6901 cggcagtgtt gatggcgtca agaactctga ctgcatgcgt taaaaattgt tcgccgggta
     6961 aggtaagttg cgccccctga cgaccacgct caaacaagcg agcgcccgtc agctgctcca
     7021 gttcattcaa tgtcttagag agcgcaggtt gactcaaatt aagggtttca gccgcgcgcc
     7081 ccaaagttcc ttgttgtgcg acagctacga atgtatgaag gtggcgcaaa cggatgcgct
     7141 gactaaacag actatttttt tccataagcg atgttaaaaa cgaagcggtg tcgctgacaa
     7201 gtgaagttgt ttgattatga taacttgatt gcaaaatatt attaacaatt aaagcaatta
     7261 tgttacagca aaatggataa tattgatgtt ttcgcggcga gatcacagtt tgtaaattct
     7321 tcccgccaga gtgaatgcgg ttacctacac tccagattac tgaccactgg aggcagacac
     7381 tatggcgaac agcatcacgg cggatgagat tcgggaacag ttttcgcagg caatgtcagc
     7441 catgtaccag caagaagttc cgcaatatgg cacgctgctg gaactggtag ctgatgtgaa
     7501 tctggctgtg ctggaaaaca atcctcaact gcacgaaaaa atggtaaatg cagacgagct
     7561 ggcgcgactg aatgttgaac gtcatggggc gattcgcgtt gggactgcac aagagcttgc
     7621 tactcttcgg cggatgtttg ccattatggg gatgtacccg gtgagctatt acgatctctc
     7681 gcaggcaggg gtgccggtac attcgacagc atttcggccc attgatgatg cttctctggc
     7741 gcgtaatccc ttccgcgttt ttacctcctt actccgcctt gagcttatcg agaacgaaat
     7801 tttgcgccag aaagcggcgg agattctgcg tcagcgcgat atcttcaccc cacgttgtcg
     7861 acaactgtta gaggaatatg agcagcaggg cggttttaac gaaacacagg cacaggagtt
     7921 tgtgcaggaa gccctggaaa cgtttcgctg gcaccagtca gcaacggtag atgaagaaac
     7981 ctatcgcgcc ttgcacaacg aacatcggtt gattgctgat gtggtctgtt ttcctggatg
     8041 ccatatcaac cacctgacgc cacgtacgct ggatattgac cgggtgcagt cgatgatgcc
     8101 tgaatgcgga attgaaccca aaattctcat cgaggggccg ccgcgccgcg aggtaccgat
     8161 tttactacgc cagaccagct ttaaagcact ggaagagacg gtgttgtttg cggggcagaa
     8221 acagggcacg cataccgcgc gctttggtga aattgagcag cgtggcgtgg cattaacgcc
     8281 gaaagggcga caactgtatg atgatcttct gcgtaacgct ggaaccgggc aggataatct
     8341 cactcaccaa atgcatttac aggaaacctt ccgcactttt cctgacagtg agtttttaat
     8401 gcgtcagcaa gggttggcat ggttccggta ccgtctgacg ccttcgggtg aggcgcatcg
     8461 tcaggcgatt catcctggag acgatccaca gcccttaatt gaacgtggtt gggtagtggc
     8521 gcaacccatc acctatgaag atttcttgcc cgttagcgcg gcggggatct tccagtcaaa
     8581 tctgggtaat gaaacgcaga cacgcagtca cggtaatgcc agtcgcgaag catttgagca
     8641 ggcgttgggt tgtccggttt tggatgagtt ccagctttac caggaagcgg aagaacgcag
     8701 taaacgtcgc tgtggtttgc tttaaattct gaccatccgc ctttgcaaaa atttgcctga
     8761 ttttacaaac gaatcaggct catcccatcg acataaaaaa aatgccgatt tatgcatatt
     8821 ctctcagttc aacaattgga ttattaataa atattgtcta gagtgagcgg tcataaataa
     8881 gcactttctt gccgctgaaa acgaccagcg cgggaccatt cacaacacca gaaggactca
     8941 ctttcaggta tggatcgtag acgatttatt aaaggttcaa tggctatggc cgccgtgtgc
     9001 ggtaccagcg gcattgcttc tcttttttct caggcggcat tcgcggcaga ttctgatatt
     9061 gccgacgggc aaacccagcg ttttgacttc tccattctac agtcaatggc gcacgactta
     9121 gcgcaaacag cgtggcgtgg tgcgcctcgt ccgttacctg acacgctggc gacaatgacg
     9181 ccgcaggctt ataacagtat tcaatacgac gccgaaaaat cgctctggca taacgttgag
     9241 aaccgtcaac tggacgctca gttcttccat atgggaatgg gattccgtcg ccgcgttcgt
     9301 atgttttctg tagatccagc aacacatctg gcgcgtgaaa ttcactttcg cccggagttg
     9361 ttcaaataca acgatgcagg tgttgatacc aaacaattag aagggcaaag cgatctcggc
     9421 tttgccggtt ttcgcgtgtt taaagccccc gaactggcgc gccgtgatgt agtatcattt
     9481 ctcggcgcga gttatttccg cgccgttgat gatacatatc aatacggttt gtcggcccgc
     9541 ggcctggcga tcgacactta caccgacagt aaagaagagt tccccgactt taccgccttc
     9601 tggtttgata cggtaaaacc gggggcaact acctttaccg tttatgcgtt gctcgatagc
     9661 gccagtatta ctggtgccta taagttcact atccattgtg agaaaagtca ggtgattatg
     9721 gatgtggaaa atcacctgta tgcgcgcaaa gacattaaac agctgggcat tgcgccgatg
     9781 accagtatgt tcagctgcgg tactaatgaa cgtcggatgt gcgacaccat tcatccgcaa
     9841 attcatgact ctgatcgtct gtccatgtgg cggggcaacg gcgagtggat ttgccgtccg
     9901 ctgaacaatc cgcaaaaatt gcagttcaat gcttacaccg acaacaaccc gaaagggttt
     9961 ggtttattgc aactggatcg tgacttctcc cattatcagg acattatggg ctggtataac
    10021 aaacgcccaa gtttgtgggt ggaaccgcgt aacaagtggg gtaagggcac catcggcctg
    10081 atggaaatcc caacaacggg cgaaacgctg gataacattg tctgcttctg gcagccagaa
    10141 aaagctgtaa aagcaggtga tgagtttgca ttccagtatc gtctgtactg gagtgcgcaa
    10201 ccgcctgttc attgcccatt agcgcgcgtt atggcgacgc gtaccggcat gggcggtttc
    10261 ccggaaggtt gggcgccagg tgaacactat cccgaaaaat gggcgcgtcg ttttgccgtc
    10321 gatttcgttg gtggtgatct gaaagctgcc gcgccaaaag gcattgagcc ggtgattacg
    10381 ctttccagtg gggaagcgaa gcaaatcgaa attctctata ttgaacccat cgatggttat
    10441 cgtattcagt ttgactggta tccgacttcg gactctactg atccggtcga tatgcggatg
    10501 tatctacgtt gtcaggggga cgctatcagt gaaacatggc tgtatcagta tttcccgcca
    10561 gcgccggata aacgtcagta tgttgacgac cgcgtgatga gttaatcgtt ttttcttcgg
    10621 caccttcttc gggaggtgcc gtctggttaa acacgatccc gctcgcattt ttccctaagt
    10681 taaatgagta atctgatggt gtgtatttca gatacacctt gtcagccact aacagggagt
    10741 gcgtatgttt ccagaatacc gagatttaat atcccgtctg aaaaacgaaa atcctcgctt
    10801 tatgtccttg ttcgataaac acaataaact tgatcatgaa attgccagaa aggaaggttc
    10861 cgacggtcga gggtacaatg cggaagtggt ccgcatgaaa aaacaaaagc tacagttaaa
    10921 agatgagatg ctcaaaatcc tgcagcagga gagcgtcaaa gaggtgtaaa ctttcctaag
    10981 ccgccagcca ggcggctttt ttaacaacag catggattga ctggagataa gatgactgaa
    11041 acgataaaag taagcgaatc acttgaatta catgctgttg cggaaagtca cgtcacacct
    11101 ctttatcagt taatctgtaa aaataaaacc tggttacagc agtcgctaaa ctggccgcag
    11161 ttcgttcaga gtgaagagga cacgcgaaaa acggtgcagg gtaatgtgat gttgcatcaa
    11221 cgcggctatg ccaaaatgtt catgattttc aaagaagatg aacttatcgg cgttatctcg
    11281 tttaatcgta ttgaaccact gaataaaact gctgaaatcg gctactggct ggacgaatct
    11341 catcaggggc aggggatcat ttctcaggcg ctgcaggcat tgattcatca ttacgctcag
    11401 tctggtgaac ttagacgctt cgtgatcaaa tgtcgggtgg acaatccgca aagcaaccag
    11461 gtcgctttgc gcaatggttt tatccttgaa ggttgcctga aacaggctga gttcctgaat
    11521 gatgcctatg atgatgtgaa cttatacgcg cgtattatcg attcataata accctgccag
    11581 cggcgttcgc gtaatgcgct cctcgccgtt aatgactttt ggaccacgga catgaaccgt
    11641 gtcgccatca aaggcttcaa cgacggcgtg gtcggttaac tccacatggt tttcaattat
    11701 caccgcacct gaaatacggc tctcaccttg tattacaacg tgctcgtcga gcagaatcgg
    11761 tccaccacgt accacggcat ttccgccaat caagacatga tgttttaaca cacaattacc
    11821 ttccacaatg gcatattccg ccacctgcga actgtaatga atcgtcggaa tggcatcttc
    11881 ttctatgcca gctttcacct gcgcgtgacc gtagacttta gcgcaatcgc atagccagac
    11941 gttgttctct tcattacctt caacactggc aaaatcaaaa acttcggcgc gatgttcaat
    12001 aaaagcatac cggacgacgg catcgccata aatttgtgcc tggtggacaa tacgcgaggc
    12061 gctaaccctt gcgcgatcat aaatttggag caggagttga tggtcgggcg ttaagccttg
    12121 tgcggcgacg atcatagaat gttgatcaat taaggcgtga ccaaatattc tacattgccc
    12181 gtaaactaat gagtcacgaa tggtgacact gtcacttata tatgcaccct ggctgatttc
    12241 actattatcg atccagacgt tatccgttgc gtaaacctct ccccacagca cgctggtgcc
    12301 ggtgatacgc gtattaccag aaatcaccgt tccgccgaac gcaatagcat tctggtcgta
    12361 aatccagcaa tttccttcct gtgctaacac cgtttcgcga tcgatccagc cgccagcggt
    12421 tcctgctatc acatcgttaa aatcgctaat ggcgataatc tgccataata acacactttt
    12481 tttagtgcca tcttcctgat aactaaaggc tcgctgttct tcacttagac gatatttgcg
    12541 catagcgttt tctcacaggt gacttactat aaccgtagca aattctgcgg ctctggctat
    12601 gctcgagaaa attccataaa atgcatttca aatatacttt ataaattaaa caaaatgagt
    12661 aagaagatgc agagcgataa agtgctcaat ttgccggcag gctactttgg tattgtgttg
    12721 gggacgatag ggatgggatt tgcctggcgc tatgccagcc aggtttggca ggtcagccac
    12781 tggttagggg atgggctggt gattctggcg atgatcatct ggggattatt aactagcgca
    12841 tttattaccc gactcatacg ctttccgcat agcgtgctgg cggaagttcg ccatccagtg
    12901 ctgagcagtt ttgtgagttt gtttccggca acgacgatgc tggtggcgat tggttttgtt
    12961 ccgtggtttc gcccactggc ggtgtgcctg ttcagttttg gtgtcgtggt tcagttggct
    13021 tatgccgcct ggcaaactgc gggattatgg cgcggatctc accctgaaga agctaccacg
    13081 cctggactgt atctgccgac agttgccaac aactttatca gcgcaatggc ctgtggtgcg
    13141 ttgggctaca ccgacgccgg tctggtgttt ttaggcgcag gcgttttctc atggctaagt
    13201 cttgaaccgg tgatcttgca gcgtctgcgc agttcgggag aattacccac ggcactgcgg
    13261 acatcactcg gcattcagct cgctcctgcg ctggtggcct gtagtgcctg gttgagcgtc
    13321 aacggcggcg agggtgatac gctggcgaaa atgctttttg gttatggact gctgcaactg
    13381 ctgtttatgc tacgtctgat gccatggtat ctctcccagc catttaatgc ttcattctgg
    13441 agtttctcgt tcggcgtatc tgcactggca accaccggtt tgcatctggg gagtggcagc
    13501 gaaaatggat ttttccatac gctggcggta ccgctgttta tctttaccaa ttttattatt
    13561 gcaatactgc tcatccgtac ttttgcgctt ctgatgcagg gaaaattgtt agtcagaacc
    13621 gagcgcgccg ttttaatgaa agcagaggac aaagaatgat cattcgtgac gaaaactatt
    13681 ttactgataa atatgaatta acccgcacac actctgaagt actggaagcg gtgaaagtgg
    13741 ttaaaccggg taaaacgctg gatctgggct gtggcaatgg tcgtaacagt ctttacctgg
    13801 cagccaatgg ttatgatgtt gacgcatggg ataaaaatgc catgagtatc gccaatgtag
    13861 agcgcattaa atccattgaa aatctggata atttacacac ccgagtcgtt gatctgaata
    13921 acctca
//
LOCUS       DAKISA010000108        13904 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.108, whole genome shotgun sequence.
ACCESSION   DAKISA010000108 DAKISA010000000
VERSION     DAKISA010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13904
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.108"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            241..1494
                     /gene="kch"
                     /locus_tag="OVW45_003425"
     CDS             241..1494
                     /gene="kch"
                     /locus_tag="OVW45_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCV5527788.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(1549..1722)
                     /locus_tag="OVW45_003426"
     CDS             complement(1549..1722)
                     /locus_tag="OVW45_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCV5527789.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1865..3325
                     /gene="cls"
                     /locus_tag="OVW45_003427"
     CDS             1865..3325
                     /gene="cls"
                     /locus_tag="OVW45_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCV5527790.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3360..3689
                     /locus_tag="OVW45_003428"
     CDS             3360..3689
                     /locus_tag="OVW45_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCV5527791.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3742..4746)
                     /gene="oppF"
                     /locus_tag="OVW45_003429"
     CDS             complement(3742..4746)
                     /gene="oppF"
                     /locus_tag="OVW45_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCV5527792.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPISALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTCCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4743..5756)
                     /gene="oppD"
                     /locus_tag="OVW45_003430"
     CDS             complement(4743..5756)
                     /gene="oppD"
                     /locus_tag="OVW45_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCV5527793.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5768..6676)
                     /gene="oppC"
                     /locus_tag="OVW45_003431"
     CDS             complement(5768..6676)
                     /gene="oppC"
                     /locus_tag="OVW45_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCV5527794.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6691..7611)
                     /gene="oppB"
                     /locus_tag="OVW45_003432"
     CDS             complement(6691..7611)
                     /gene="oppB"
                     /locus_tag="OVW45_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCV5527795.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIVALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7697..9328)
                     /gene="oppA"
                     /locus_tag="OVW45_003433"
     CDS             complement(7697..9328)
                     /gene="oppA"
                     /locus_tag="OVW45_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCV5527796.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            9612..9908
                     /locus_tag="OVW45_003434"
     CDS             9612..9908
                     /locus_tag="OVW45_003434"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5527797.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            complement(10066..10713)
                     /gene="ychE"
                     /locus_tag="OVW45_003435"
     CDS             complement(10066..10713)
                     /gene="ychE"
                     /locus_tag="OVW45_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCV5527798.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11191..13866
                     /gene="adhE"
                     /locus_tag="OVW45_003436"
                     /gene_synonym="adhC"
     CDS             11191..13866
                     /gene="adhE"
                     /locus_tag="OVW45_003436"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCV5527799.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3657 a         3436 c         3233 g         3578 t
ORIGIN      
        1 ttatagccgg tcagtgcatt cactaccaca agcagataat aactttatct gtatttcttt
       61 atatgatgtg agcatattca ggaaaaatga cctttcctga ggtacagttg ctcgctcaaa
      121 tcctttgatt actgcggata aagtcatcta ctattgttta acacattcac gcgctgcatg
      181 tatgttcaga aaatgtgcac gctaagataa ttaaacaaaa ttgtggttac agaggtatca
      241 gtgagtcact gggctacatt caaacaaaca gcaacaaatt tatgggtgac ccttcggcac
      301 gacattctcg cgctggccgt ctttttaaat ggattgctta tttttaaaac aatctatggt
      361 atgtcggtca atttgcttga tattttccat atcaaagcat tttcagagtt ggatctctcc
      421 ttgctggcaa acgccccact atttatgctc ggcgtctttc ttgtcctgaa ctccattggc
      481 ttactgttcc gggcaaagct cgcatgggca atcagtatca ttttgttgtt gatagcgcta
      541 atttacaccc tgcattttta tccctggctg aaatttagta ttggattttg catttttacg
      601 ctggtgtttt tgctgatact gcgcaaagac ttctcccaca gtagcgccgc agccgggaca
      661 atttttgcat ttattagttt cacgacatta ctgttttact caacctacgg tgcgctttat
      721 ttaagcgaag gttttaatcc gcgaatagaa agtttgatga ccgcgttcta tttttcgata
      781 gaaaccatgt caaccgtcgg ctatggcgat attgtccctg tttctgaatc agcacgattg
      841 ttcactattt cggtcattat ttccggcatt accgtttttg ccacatctat gacctcaatt
      901 tttggtccgc ttatccgcgg gggattcaac aaacttgtaa aaggaaacaa tcatacaatg
      961 catcgtaaag atcattttat tgtttgcgga cattcgattc tcgccatcaa tacgatcctg
     1021 caactgaatc aacgcggaca aaacgtaacg gttatcagca acttgcctga agatgatatc
     1081 aagcaacttg agcaacgctt aggcgataac gctgatgtta tccccggtga cagtaatgac
     1141 agttcagtat taaagaaagc gggaatcgat cgttgccggg ccattctggc gctgagtgat
     1201 aacgatgcag ataacgcgtt tgttgtactc tcggcaaaag atatgagcag tgatgtcaaa
     1261 acagttctcg ccgtcagtga tagcaaaaac ctgaataaga ttaagatggt acatccggat
     1321 atcattctct caccgcaact gtttggcagc gaaattctgg cgcgagtttt aaatggtgaa
     1381 gagattaata atgatatgct cgtttcaatg ttgttgaact ccggtcatgg cattttcagc
     1441 gataacgatg aacaagaaac gaaagctgac agtaaagaat cagtgcaaaa atagagttta
     1501 aagacgaaag agtaaactta ttgggcaccg caatggtgcc cagtatattt agatattgta
     1561 gatcgcaaac aataacgagt tacgctgttt gtttagaatg cacttcctga tgctgtggat
     1621 acgcatattt cggcgcgatt gcccctcaag ccaacgcgat ttacgtcggc tagcctgacg
     1681 ctgcatgcgc cagcgcccca cttccgttct actccgcttc atgttaacta ctctatgcaa
     1741 taacagaatg gtcattatac ccccaccccc gacgctgacc agtgcttttc ccgcgttttt
     1801 atttgccaga tgaatccata tgcgtaaact cataacaatg cgctttcaaa aggatttcta
     1861 atctatgaca accgtttata cgttggtgag ttggttggcc attctgggat actggttgct
     1921 cattgcaggc gtaactttac gcattctaat gaaacgacgc gcagttccct ccgcgatggc
     1981 ctggctgttg attatttaca ttctgccgtt agtcggaatt attgcctatc ttgccgttgg
     2041 cgagctccat ttaggcaaac gccgcgctga gcgcgccaga gcgatgtggc cttccaccgc
     2101 aaaatggctt aacgacctta aagcctgtaa gcatatcttc gccgaagaaa atagcagtgt
     2161 cgctgcgcca ttattcaagc tttgcgagcg tcgtcagggg atcgctgggg tcaaagggaa
     2221 tcagctacaa ctgatgaccg agtcagatga tgtgatgcag gcgttaatcc gcgacatcca
     2281 gctcgcgcgc cataatattg agatggtgtt ttatatctgg cagcccggcg gcatggcgga
     2341 ccaggtggct gaatcattaa tggcggctgc acggcgtggt attcattgcc gattgatgct
     2401 cgactccgcc gggagtgtgg cttttttccg cagcccgtgg cccgagctaa tgcgtaatgc
     2461 cggtattgaa gtggtcgaag ccttaaaggt caatctgatg cgtgtgtttt tacgccgtat
     2521 ggacctgcgc caacatcgca agatgatcat gatcgataat tacatcgcgt acaccggcag
     2581 catgaatatg gtcgatcctc gctacttcaa acaagatgcg ggcgtagggc aatggattga
     2641 tctgatggcg cgtatggaag gccccatcgc caccgcgatg gggattattt attcctgcga
     2701 ctgggagatt gaaaccggaa aacgtattct gccgccacca ccagatgtca atattatgcc
     2761 gtttgaacag gccagcggtc acaccattca cacaattgct tctggccccg gctttccgga
     2821 agatctcatt caccaggcat tattgactgc ggcttattcg gcgcgtgaat atttgatcat
     2881 gacaacgccc tactttgtgc caagcgatga tttacttcat gcgatttgca cggcggcgca
     2941 gcgcggggtg gatgtcagta ttatccttcc gcgaaaaaat gactcgatgc tggtcggctg
     3001 ggccagtcgc gcattcttta cggaactgct tgctgctggg gttaaaattt atcagtttga
     3061 aggcgggtta ctgcatacca agagcgtgct ggtcgatggc gaactaagtc tggttggcac
     3121 cgttaacctt gatatgcgta gtctgtggct gaatttcgag attaccctgg caatcgacga
     3181 taaaggtttt ggtgcagacc ttgccgccgt tcaggacgat tatatttcgc gttcacgtct
     3241 gctcgatgcc cgtttatggc taaaacgtcc attatggcaa cgtgtcgccg agcgactgtt
     3301 ttacttcttc agcccgttgc tgtaaaacgt cgccaacaga ggttaaacag gtagtgacta
     3361 tggatatgga tctaaacaat cgcctgactg aagatgaaac gcttgagcag gcctacgata
     3421 tttttctcga actggctgcc gacaatctcg acccagctga tgttctgctg ttcaatcttc
     3481 agtttgaaga gcgcggcggc gcggagttat tcgatccggc ggaagactgg caggaacatg
     3541 ttgatttcga tttgaacccg gacttttttg ccgaagtggt gattggtctg gcggacagtg
     3601 aggatggtga aatcaacgat gttttcgccc gcattctgtt atgccgcgaa aaagatcaca
     3661 aactttgcca tatcatctgg cgggaataaa taatcgcttt cggcgtgaaa aagggctgac
     3721 aactgtcagc ccttattgtg tttaaagcgg atcgactttc aggcaagaaa cggcgtgtct
     3781 gaaactccct tccagcacag gacgtgtttt ggcgcactcc ggaccggcaa tcgggcaaca
     3841 ggtacggaaa acacaaccgg aaggcgggtt gatcggcgac ggtaattccc cttccagtaa
     3901 ctggatggtt ttgttcttct ccagatccgg atcaggtatg gggactgccg acatcaatgc
     3961 cctggtgtaa ggatgtagtg gattgtggta gacctcatca taggtcccca gttctaccgc
     4021 atggccgaga tacatcacca acacacgatc ggaaatgtgt tttaccacgg ccaggtcatg
     4081 agcgataaaa attaatgaca atcccatctc acgttgcagc tgctggagca ggttgaccac
     4141 ctgcgcctga attgacacgt ccagcgccga aatcggctca tcgcagataa tcagcttcgg
     4201 ttcaagaata agagcacgag caatcccgat acgctggcac tgcccaccgg agaactcatg
     4261 cggatagcgg ttaatcaggt taggcaataa cccgactttc agcatcatcg ccttcacgcg
     4321 ctcgcgaact tcctggcgtg acattttcgg atgataagta cgcagtggtt cagcgatgat
     4381 ctcgccgatg gtcatacgcg ggtttagcga tgccaacgga tcctggaaaa tcatctgaat
     4441 atcactgcga acggcacgcc attcatcggg cttcatgccc agcaactctt tacctaacca
     4501 ggcaacatga ccgtcggtcg ctttaaccag gccaatgatg gcgcgagcaa aggtggactt
     4561 accgcatccc gattccccta ccacacctaa tgtttcccct tcatacaggc gaagcgtgac
     4621 accatcgacg gctttgagag ttttcggcgg ttgccagaac cactgtttgc catctttgat
     4681 ttcaaagtgc actttcagat cggcgatttc gaggaggact tttcttcctt cggttacagc
     4741 attcataaca gttcctccac cggtttaaag caagcacgca gacggccagg cgtaaactct
     4801 tccagcggcg gtgcgctact acaaatttcc atcgcatgcg gacaacgtgg ctggaacggg
     4861 caacctttcg gtaatcgcag caggtttggc ggattaccag ggatggtcaa cattgtttca
     4921 ccttccgcat cgagacgcgg caccgcgttg agcaaaccga tagaataagg atgaacgggt
     4981 tgataaaaga catcgcgcgc gttgccatat tccatcgtgc gcccggcgta cattaccagc
     5041 actttgtcgc agatccccgc caccacgcca agatcgtggg taatcataat gatggcggta
     5101 ttaaattccc gcttcagttc atttaatagc gtcatgatct gcgcctgtac ggtgacgtcc
     5161 agcgcagtag tgggttcatc cgcaatcagc agcttaggtc gacatagcaa tgccatcgca
     5221 atcataactc gctgacgcat gccaccagaa aattcgtgcg ggtacatttt catgcgttta
     5281 cgtgcttccg gcatttttac cgcatcgagc atccgcaccg actcttcaaa cgcctcagct
     5341 ttgctcatgt tcttatgcag catcagcact tccatcaact gctcaccgac gcgcatatag
     5401 ggattcaacg aagtcattgg gtcctgaaaa atcattgaga tttgttcagc gcgcagttta
     5461 ttgagttcac gctctggcaa attgaggatt tcacgcccat tgaaggttgc cgatccgcca
     5521 atacgtccgt tggcagccaa caggcccatc aacgcaaatg cagtttgcga tttacccgaa
     5581 ccagactcac ctacaatgcc cagcgtttct ccggcacgta gagaaaaatt caaatcattg
     5641 accgccgtga catcgccgtc cggggtacta aaggtgacac gcaaatcttt cacgttcagc
     5701 agtgcgtcag cctgttgttg tgcgagcggc acagttgcag tttcaattac gctcatggct
     5761 gcactcctta acgatctttc gggtcgagag catcacgcaa gccatcgccg ataaagttga
     5821 aacaaaacag cgtcaccacg aggaatcccg ctgggaacaa caataaccat ggagagactt
     5881 ccatcgagtt cgcgccatca ctcagcaatg caccccagct gcttaacggc tcttgcgtac
     5941 ccaaccccag gaagctaagg aaagattcaa agaggatcat gctgggcacc agaagtgatg
     6001 cgtagaccac caccacaccg agtacattcg gcacaatgtg gcgaataaca atgcccgatg
     6061 tcgatacacc gccaacttgt gccgcctcaa taaactcttt gcgcttcaga ctcagggttt
     6121 gtccacgcac aatacgagcc atatccagcc aggaaaccat gccaatcgcc acgaaaatca
     6181 gcaggatgtt ttgaccgaaa aaggtcacaa gcaaaatgac gaagaacatg aatgggaagg
     6241 agttgaggat ttccagcaga cgcatcatta ccgaatccac tttaccgccc agataaccgg
     6301 aaagcgaacc gtaaagtgtc cccacgacca ctgccaccag tgccgcagca acaccgacca
     6361 tgagtgagat acgcccgcca atcgcaacgc gcacaagcag gtcgcgaccg gatgagtcag
     6421 taccaaagta gtgaccggac tccatatccg gggcgctgga catcatcgcc cagtcagtat
     6481 cgtcataggc aaactgcgaa agcatcggtg ccaggattac aaataacgcg atcagcacca
     6541 gcactatcag actggcaacc gccgcacggt tatgcataaa acgtcgacgt gcgtcctgcc
     6601 acaagctgcg cccttcgacc tccagctttt cactgaaatt ttccagcgtc tcgctgtttt
     6661 tcttacttaa catcatcgcg agctccagta tcagtaacgg attttcgggt cgataaccgc
     6721 atatagcaca tcgacaatgg cattaaacaa aatggttaaa gcaccaacca ggatggtcag
     6781 gcttaacact aaggaatagt cacggttcaa tgcgccatta acgaacagtt gtccaatccc
     6841 cggcaaacca taaatggttt cgataaccat agaaccggta ataatgccga caaatgcggg
     6901 gcccatataa gagagcacgg gtaacagagc aggttttaat gcgtggcgta aaatgatccg
     6961 ccgcataggt aaccctttcg cccgggcagt acgaataaag ttggagtgta atacttcaat
     7021 catagagcca cgggtaatac gcgcaatact ggcgatataa gcgagtgaca acgccaccat
     7081 cggcaatatc atgaatttaa gcgccccacc attccagcca ccgcccggca gccaatgcaa
     7141 aatgatcgca aatatcatga ctaataatgg cgcaaccaca aaactgggga taacaacccc
     7201 ggtcattgcc agccccatca cggtatagtc ccatttggtg ttttgtttta atgcgacaat
     7261 aacgccagca ctaacaccca gtattaccgc aaggaaaaat gcggcggctc ccagtttggc
     7321 agaaacggga aaactggatg caaccaggtc attgaccgaa taatctttat atttaaacga
     7381 tggaccgaaa tcaccgtgcg ccagttgttt caggtagctg aaatactgtg tcatgatcgg
     7441 atcattaaga tgatatttcg cttcgatatt ggccatcact tccggcggta aagtacgttc
     7501 gccggtaaaa gggcttcccg gcgcgaggcg catcataaag aacgaaatag taataagaat
     7561 aaatagcgtc ggaatcgctt ccagacagcg acgtagaata aattttaaca ttgccctacc
     7621 ttctggcctg tgccttctgt aatgcgataa aaatcagaca ccgtggagca ggacactcct
     7681 gccccacgta ttgccattag tgcttcacaa tgtacatatt ccgggtgtag gtattatcca
     7741 gcggatcttt gccggtatag ccaccaaccc acggtttcac cagacgcgca ttcacgtagt
     7801 aataaacagg aacaatggcc gaatccttat ccagctgttg ttctgcttta gtgtacagag
     7861 ctgtgcgctg cgcctcgtca gtcactttca gcgtttccgc cataatgctg tcaaaggccg
     7921 ggctcttata atgcgcggta ttcatcgagc tgttcgacag catggtgttc aggaaggaag
     7981 ttggttcgtt gtagtctgca caccagcctg cacgggccac atcaaaagtg ccctggtgac
     8041 gggtgtcgag gaacgttttc cactcctggt taaccagttt gacgtttaca ccaatgtttt
     8101 tcttccacaa tgaagaggca gcaatcgcca gctttttatg cagatcagag gtgttataca
     8161 gcaggttgat ggtcaacggt ttgtctgcgg tataacccgc ttcagccagc agttttttcg
     8221 cttcttcgtt acgtttttcc tggctccagc caaaccattc tggctgagtc aattttgcgc
     8281 catcagtata cggtggagtg taaccatagg cgggcatgtc gccctgcgct ttcactttat
     8341 taacaatgat atcgcggtcc atacctagtt tcagtgcggt acgcacacgc acatcgttga
     8401 atggcggttt ctggttgtta atttcgtaat agtaagtgca caggtatgga tcaacgtgaa
     8461 cttcgtccgg gatctctttt ttcagcttct ggaacaattc gatcggcatg ttgttatacg
     8521 tcatgtcgat ttcaccacta cggtagcggt tgacatcggt aacttcagaa gcaataggca
     8581 aataggttac ctggttaata acggttttcg cgttgttcca gtaggtcggg ctgcgttcaa
     8641 gaacgattcg ttcgtttacg acccaatctt ttaaggtata ggcaccgttg gtgacgatat
     8701 taccaggctg ggtccatttt tcgccgaatt tctcgatagc ggcttttggc accggtgaag
     8761 ttgatgggtg aacaagtaat ttatagaagt acggaacggg ttcacttaag gtgacttcta
     8821 atgtgtgatc atcaatagct ttcacgccga gatcggtaat cggttttttc ccttcaagaa
     8881 tttcatcaat accggcgata tgcccatatt gcagataact ggcatacgga gaagcagtgt
     8941 tcggatcaac agaacgttgc cagctataca caaagtcttg tgctgtgact ggcgtgccat
     9001 cagaccattt cgcatcttta cgcaaatgga aggtccagac tttcgcgtct ttattatccc
     9061 aggattcagc gacgccaggt gctggatgac cgtcaagatc gctgaccagt aagccttcaa
     9121 acaggtctcg gctgatatta gactccggaa caccttcaat tttgtgcgga tctaatgact
     9181 gaacttctga accattgtta cgtaccagtg tttgtttttc cgccagtgtg acgcctgcgg
     9241 gtacatcagc tgccagcgcg acattccctg ccattagcgc agccagaacg ccagctgcta
     9301 ctaaacttct cttggtgatg ttggtcattg ttttttggac tccctcatta taattactgg
     9361 tattaaccag catgtgtaat cccggattgg gaccctgcac aggctcggag actttctgct
     9421 gtcaggttac tttatcgtta cgtattacta tcaccgactt tatttattct ggtgattcag
     9481 acgatcacct tatgtcgatt cgtactgtgt ctcccctgtc gagacacatt attctttcgc
     9541 gacgctcttt aattgttaat tattctcatt accaataaag aatcgtctgg cggttgcccg
     9601 gaaagtacca aatgcggttc ttactcgcca atacattttg caaaattgtt aagcaattct
     9661 cttttattga ccgtatcaga atctatagct tgcatccatg ccttaacaaa catctttcaa
     9721 ttcaatcaaa tagagattgg ctaagcatgt ctatcttttc tgattacagt tccagtagtg
     9781 aaatgcacaa taatttaaca attgattatt atttagcact ttcctctacc aaaggcagtg
     9841 gaattactaa tatcatttca attattcttc agcaagccca ggattatgat gtggcgaaaa
     9901 taacatgatg gtgaggagaa gcagttaagg aaatccttat aactaactgt aatttgagaa
     9961 gaatattttt acctattcct tttgcttttg ataactgcgc gggaacaaag taaaaataat
    10021 gacgaagtaa acggagcatg gcagctccgt ttcatttgaa aggaattaat taagcaggcc
    10081 ggggaaaatc cccttaatac cagtaacgat aaattcaatc cccaatgcca tcagcaatag
    10141 ccccataata cgcgtaatca cgttgatgcc ggtctggcgt aaaacccgta ccagccacgg
    10201 tgccatgcgg aacaatcccc agcaacataa agcgaacaat gcaatagcca caaagaaacc
    10261 aaacagatag ctaatgctgt gataacgcgt accccagacg atggtagaac tgatcgcccc
    10321 tggccccgcc atcaacggca acgccaatgg caccacacca atgctttcac gtaccgcggt
    10381 ttctgatttt tcttgcttgt tctgtttatc ctcgccaagc ttgccgctga tcatcgacat
    10441 cgctatcgtc accaccagga tgccccctgc gatacggaac gaatcaattg atataccaaa
    10501 aagttgcaga atcgtgtcgc cgagaaaaag cgagatccac aagataatgg ccacagacag
    10561 gttggctgta aggttagttt tgtttcgcgc tgccgctgtc tgataactgg tcatgctgat
    10621 aaagacggga ataatcccta ccgggttgac cagcgcaaat aacccgatga aaaatttgaa
    10681 gtaaacggga aaatcaaaaa aggtctgaat cacggttagc tccgaagcaa aagccggata
    10741 atgttagcca taaattaagg ttgaaaagac gcgctgacaa tacgcctttt gacagcattt
    10801 ttcacctcct aactacttaa aattgctatc attcgttatt gttatctagt tgtgcaaaac
    10861 atgctaatgt agccaccaaa tcatactaca atttattaac tgttagctat aatggcgaaa
    10921 agcgatgctg aaaggtgtca gcttagcaaa aatttgattt ggatcacgta atcagtaccc
    10981 agaagtgagt aatcttgctt acgccaccta gaagtgacgc attagagata ataactctaa
    11041 tgtttaaact cttttagtaa atcacagtga gtgtgagcgc gagtaagctt ttgattttca
    11101 taggttaagc aaatcatcac cgcactgact atactctcgt attcgagcag atgatttact
    11161 aaaaaagttt aacattatca ggagagcatt atggctgtta ctaatgtcgc tgaacttaac
    11221 gcactcgtag agcgtgtaaa aaaagcccag cgtgaatatg ccagtttcac tcaagagcaa
    11281 gtagacaaaa tcttccgcgc cgccgctctg gctgctgcag atgctcgaat cccactcgcg
    11341 aaaatggccg ttgccgaatc cggcatgggt atcgtcgaag ataaagtgat caaaaaccac
    11401 tttgcttctg aatatatcta caacgcctat aaagatgaaa aaacctgtgg tgttctgtct
    11461 gaagacgaca cttttggtac catcactatc gctgaaccaa tcggtattat ttgcggtatc
    11521 gttccgacca ctaacccgac ttcaactgct atcttcaaat cgctgatcag tctgaagacc
    11581 cgtaacgcca ttatcttctc cccgcacccg cgtgcaaaag atgccaccaa caaagcggct
    11641 gatatcgttc tgcaggctgc tatcgctgcc ggtgctccga aagatctgat cggctggatc
    11701 gatcaacctt ctgttgaact gtctaacgca ctgatgcacc acccagacat caacctgatc
    11761 ctcgcgactg gtggtccggg catggttaaa gccgcataca gctccggtaa accagctatc
    11821 ggtgtaggcg cgggcaacac tccagttgtt atcgatgaaa ctgctgatat caaacgtgca
    11881 gttgcatctg tactgatgtc caaaaccttc gacaacggcg taatctgtgc ttctgaacag
    11941 tctgttgttg ttgttgactc tgtttatgac gctgtacgtg aacgttttgc aacccacggc
    12001 ggctatctgt tgcagggtaa agagctgaaa gctgttcagg atgttatcct gaaaaacggt
    12061 gcgctgaacg cggctatcgt tggtcagcca gcctataaaa ttgctgaact ggcaggcttc
    12121 tctgtaccag aaaacaccaa gattctgatc ggtgaagtga ccgttgttga tgaaagcgaa
    12181 ccgttcgcac atgaaaaact gtccccgact ctggcaatgt accgcgctaa agatttcgaa
    12241 gacgcggtag aaaaagcaga gaaactggtt gctatgggcg gtatcggtca tacctcttgc
    12301 ctgtacactg accaggataa ccaaccggct cgcgtttctt acttcggtca gaaaatgaaa
    12361 acggctcgta tcctgattaa caccccagcg tctcagggtg gtatcggtga cctgtataac
    12421 ttcaaactcg caccttccct gactctgggt tgtggttcct gggggggtaa ctccatctct
    12481 gaaaacgttg gtccgaaaca cctgatcaac aagaaaaccg ttgctaagcg agctgaaaac
    12541 atgttgtggc acaaacttcc gaaatctatc tacttccgcc gtggctccct gccaatcgcg
    12601 ctggatgaag tgattactga tggccacaaa cgtgcgctca tcgtgactga ccgcttcctg
    12661 ttcaacaatg gttatgctga tcagatcact tccgtactga aagcagcagg cgttgaaact
    12721 gaagtcttct tcgaagtaga agcggacccg accctgagca tcgttcgtaa aggtgcagaa
    12781 ctggcaaact ccttcaaacc agacgtgatt atcgcgctgg gtggtggttc cccgatggac
    12841 gccgcgaaga ttatgtgggt tatgtacgaa catccggaaa ctcacttcga agagctggcg
    12901 ctgcgcttta tggatatccg taaacgtatc tacaagttcc cgaaaatggg cgtgaaagcg
    12961 aaaatgatcg ctgtcaccac cacttctggt acaggttctg aagtcactcc gtttgcggtt
    13021 gtaactgacg acgctactgg tcagaaatat ccgctggcag actatgcgct gactccggat
    13081 atggcgattg tcgacgccaa cctggttatg gacatgccga agtccctgtg tgctttcggt
    13141 ggtctggacg cagtaactca cgccatggaa gcttatgttt ctgtactggc atctgagttc
    13201 tctgatggtc aggctctgca ggcactgaaa ctgctgaaag aatatctgcc agcgtcctac
    13261 cacgaagggt ctaaaaatcc ggtagcgcgt gaacgtgttc acagtgcagc aactatcgcg
    13321 ggtatcgcat ttgcgaacgc cttcctgggt gtatgtcact caatggcgca caaactgggt
    13381 tcccagttcc atattccgca cggtctggca aacgccctgc tgatttgtaa cgttattcgc
    13441 tacaatgcga acgacaaccc gaccaagcag actgcattca gccagtatga ccgtccgcag
    13501 gctcgccgtc gttatgctga aattgccgac cacttgggtc tgagcgcacc gggcgaccgt
    13561 actgctgcta agatcgagaa actgctggca tggctggaaa cgctgaaagc tgaactgggt
    13621 attccgaaat ctatccgtga agctggcgtt caggaagcag acttcctggc gaacgtggat
    13681 aaactgtctg aagatgcatt cgatgaccag tgcaccggcg ctaacccgcg ttacccgctg
    13741 atctccgagc tgaaacagat cctgctggat acctactacg gtcgtgatta tgtagaaggt
    13801 gaaactgcag cgaaaaaaga agccgctccg gctaaagctg agaaaaaagc gaaaaaatcc
    13861 gcttaatcag tagcgctgtc tggcaatata aacggcccct tctg
//
LOCUS       DAKISA010000109        13649 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.109, whole genome shotgun sequence.
ACCESSION   DAKISA010000109 DAKISA010000000
VERSION     DAKISA010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13649
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.109"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..732)
                     /gene="dsbG"
                     /locus_tag="OVW45_003437"
     CDS             complement(<1..732)
                     /gene="dsbG"
                     /locus_tag="OVW45_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCV5527800.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWILDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNII"
     gene            1104..1667
                     /gene="ahpC"
                     /locus_tag="OVW45_003438"
     CDS             1104..1667
                     /gene="ahpC"
                     /locus_tag="OVW45_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCV5527801.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1838..3403
                     /gene="ahpF"
                     /locus_tag="OVW45_003439"
     CDS             1838..3403
                     /gene="ahpF"
                     /locus_tag="OVW45_003439"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001488524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCV5527802.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTV"
     gene            complement(3524..3952)
                     /gene="uspG"
                     /locus_tag="OVW45_003440"
     CDS             complement(3524..3952)
                     /gene="uspG"
                     /locus_tag="OVW45_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCV5527803.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVIR"
     gene            4173..5411
                     /locus_tag="OVW45_003441"
     CDS             4173..5411
                     /locus_tag="OVW45_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HCV5527804.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDILGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            complement(5642..6052)
                     /gene="rnk"
                     /locus_tag="OVW45_003442"
     CDS             complement(5642..6052)
                     /gene="rnk"
                     /locus_tag="OVW45_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCV5527805.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(6282..7088)
                     /gene="rna"
                     /locus_tag="OVW45_003443"
     CDS             complement(6282..7088)
                     /gene="rna"
                     /locus_tag="OVW45_003443"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCV5527806.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKTVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            complement(7202..8665)
                     /gene="citT"
                     /locus_tag="OVW45_003444"
     CDS             complement(7202..8665)
                     /gene="citT"
                     /locus_tag="OVW45_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HCV5527807.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLSVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            complement(8734..9594)
                     /gene="citG"
                     /locus_tag="OVW45_003445"
     CDS             complement(8734..9594)
                     /gene="citG"
                     /locus_tag="OVW45_003445"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCV5527808.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILT"
     gene            complement(9569..10120)
                     /gene="citX"
                     /locus_tag="OVW45_003446"
     CDS             complement(9569..10120)
                     /gene="citX"
                     /locus_tag="OVW45_003446"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HCV5527809.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRTLAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRLEALLNDVDACNVN"
     gene            complement(10124..11656)
                     /gene="citF"
                     /locus_tag="OVW45_003447"
     CDS             complement(10124..11656)
                     /gene="citF"
                     /locus_tag="OVW45_003447"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCV5527810.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANQEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            complement(11667..12575)
                     /gene="citE"
                     /locus_tag="OVW45_003448"
     CDS             complement(11667..12575)
                     /gene="citE"
                     /locus_tag="OVW45_003448"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCV5527811.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            complement(12572..12868)
                     /gene="citD"
                     /locus_tag="OVW45_003449"
     CDS             complement(12572..12868)
                     /gene="citD"
                     /locus_tag="OVW45_003449"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCV5527812.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            complement(12883..>13649)
                     /gene="citC"
                     /locus_tag="OVW45_003450"
     CDS             complement(12883..>13649)
                     /gene="citC"
                     /locus_tag="OVW45_003450"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCV5527813.1"
                     /translation="TEYEALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRH
                     PGNKIGCIVMNANPFTNGHRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGT
                     ADIPRLTVHRGSEYIISRATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRF
                     VGTEPFCRVTAQYNQDMRYWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKN
                     DLTAIAPLVPAVTLHYLQNLLEHSRQDAAARQKTPA"
BASE COUNT         3346 a         3459 c         3641 g         3203 t
ORIGIN      
        1 aatgatatta agcgttttct gatcgggcaa ccccacggcc tgttgcagcg tattttcctt
       61 actcatgtaa tagatagccg gcgtgacatt tgcccccaga tcgtccatca gtttctcatt
      121 gtcacttaac actttcattt gctctgtact tacgtttgca ggcacgttta gcttaagctt
      181 gccaccagag gcttcatatt gttgccaggt ttttgcggga tctttggagg caagaattgc
      241 cgctgctgtc gccgggcttt ctggcttgat aaccccaacc aacaatgttc ttaattgcac
      301 tttgccagaa tctacccacg ggcgcgcctg ctgccagaac tgtttacaat atgggcagaa
      361 cggatcggcg aagacgtaga caatgaccgg cgcatctttt ttaccgtcga ggatccagtg
      421 ggattgttcc atccgttgcc acatttcgcg tccggctggt gcgtaaattt ctttttcgat
      481 aagtgtgtta ctcaggtttt cacctttctc gttgtacatg taaccagaga tagcgtgctt
      541 accatcggga gtcaggtaga tggtgacgcc catatcctga tactttccga gataaccttt
      601 cattcctccg ggggcatcga acgttttgat gattgtaatg ccctgttttt caatcgcttt
      661 tactggggaa ggaagttcct ctgcgaaggc gattgcagga agcagagcca gtaaaagtat
      721 cttttttaac attaatttgt ccttttcagt cagtgcaaaa gtcgagtaaa aggcataacc
      781 tatcactgtc ataggtaaga gcttagatca ggtgattgcc ctttgtttat gagggtgttg
      841 taatccatgt cgttgttgca tttgtaaggg caacacctca gcctgcaggc aggcgctgaa
      901 gataccaaag ggtagttcag attacacggt cacctggaaa gggggccatt ttacttttta
      961 tcgccgctgg cggtgcaaag ttcacaaagt tgtcttacga aggttgtaag gtaaaactta
     1021 tcgatttgat aatggaaacg cattagccga atcggcaaaa attggttacc ttacatctca
     1081 tcgaaaacac ggaggaagta tagatgtcct tgattaacac caaaattaaa ccttttaaaa
     1141 accaggcatt caaaaacggc gaattcatcg aaatcaccga aaaagatacc gaaggccgct
     1201 ggagcgtctt cttcttctac ccggctgact ttactttcgt atgcccgacc gaactgggtg
     1261 acgttgctga ccactacgaa gaactgcaga aactgggcgt agacgtatac gcagtatcta
     1321 ccgatactca cttcacccac aaagcatggc acagcagctc tgaaaccatc gctaaaatca
     1381 aatatgcgat gatcggcgac ccgactggcg ccctgacccg taacttcgac aacatgcgtg
     1441 aagatgaagg tctggctgac cgtgcgacct tcgttgttga cccgcagggt atcatccagg
     1501 caatcgaagt taccgctgaa ggcattggcc gtgacgcgtc tgatctgctg cgtaaaatca
     1561 aagcagctca gtacgtagct tctcacccag gtgaagtttg cccggctaaa tggaaagaag
     1621 gtgaagcaac tctggctccg tctctggacc tggttggtaa aatctaaatt tccttcgtct
     1681 ttcgcgctac caactccggc gcgaaatact tcggaaattc acctaattct tcgggtgctg
     1741 cggcacccga ttttcttccc cgcaccatga tgcaagctgc atccaggtag ccgtagaggc
     1801 cgcttgcatg atgatgttta aagcccagga gataaacatg ctcgacacaa atatgaaaac
     1861 tcaactcaag gcttaccttg agaaattgac caagcctgtt gagttaattg ccacgctgga
     1921 tgacagcgct aaatcggcag aaatcaagga actgttggct gaaatcgcag aactgtcaga
     1981 caaagtcacc tttaaagaag ataacagctt gccggtgcgt aagccgtctt tcctgatcac
     2041 caacccaggt tccaaccagg ggccacgttt tgcaggctcc ccgctgggcc acgagttcac
     2101 ctcgctggta ctggcgttgc tgtggaccgg tggtcatccg tcgaaagaag cgcagtctct
     2161 gctggagcag attcgccata ttgacggtga ttttgaattc gaaacctatt actcgctctc
     2221 ttgccataac tgcccggacg tggtgcaggc gctgaacctg atgagcgtac tgaacccgcg
     2281 catcaagcac actgcaattg acggcggcac cttccagaac gaaatcaccg atcgcaacgt
     2341 gatgggcgtt ccggcagtgt tcgtaaacgg gaaagagttt ggtcagggcc gcatgacgtt
     2401 gactgaaatc gttgccaaaa ttgatacggg cgcggaaaaa cgtgcggcag aagagctgaa
     2461 caagcgtgat gcttatgacg tattaatcgt cggttccggc ccggcgggtg cagcggcagc
     2521 aatttactcc gcacgtaaag gcatccgtac cggtctgatg ggcgaacgtt ttggtggtca
     2581 gatcctcgat accgttgata tcgaaaacta catttctgta ccgaagactg aagggcagaa
     2641 gctggcaggc gcactgaaag ttcacgttga tgaatacgac gttgatgtga tcgacagcca
     2701 gagcgccagc aaactgatcc cagcagcagt tgaaggtggt ctgcatcaga ttgaaacagc
     2761 ttctggcgcg gtactgaaag cacgcagcat tatcgtggcg accggtgcaa aatggcgcaa
     2821 catgaacgtt ccgggcgaag atcagtatcg caccaaaggc gtgacctact gcccgcactg
     2881 cgacggcccg ctgtttaaag gtaaacgcgt agcggttatc ggcggcggta actccggcgt
     2941 ggaagcggca attgacctgg cgggtatcgt tgagcacgta acgctgctgg aatttgcgcc
     3001 agaaatgaaa gccgaccagg ttctgcagga caaactgcgc agcctgaaaa acgtcgacat
     3061 tattctgaat gcgcaaacca cggaagtgaa aggcgacggc agcaaagtcg ttggtctgga
     3121 atatcgagat cgtgtcagcg gcgatattca caacatcgaa ctggccggta ttttcgtgca
     3181 gattggtctg ctgccgaaca ccaactggct cgaaggcgca gtcgaacgta accgcatggg
     3241 tgagattatc attgatgcga aatgcgaaac caacgtcaaa ggcgtattcg cagcgggtga
     3301 ctgtacgacg gttccgtaca agcagatcat catcgccact ggcgaaggtg ccaaagcctc
     3361 tctgagtgct tttgactacc tgattcgcac caaaactgta taagaagaag taagattcac
     3421 ctgcaattgc ttagccgccg gggtcaaacc tggcggcttt tttatggcat taaaaagccc
     3481 tgccgggatg ttccaggcag ggcggcataa atacttttat ctgttagcga atgaccagta
     3541 caggcagatt ggcgtggcgg atcacgctcg aggcgttaga acctaacaga tgggtcgaaa
     3601 tcgatgggtt gcgagaaccg ataactacaa catcagcccc cagttcttct gccaactcat
     3661 tgacttcatc ccgcacgcta ccaaaacgga catgttgttt aatgcgggaa ggatcgatgg
     3721 tgaagtggct gaccatcgtt tgcagacgtt cttctgcttc atgttgcaga tgctcttcaa
     3781 aacgacgcac atcagcggca aaacggtgca ggctcaggct ggctgacccg ggtagtacgt
     3841 gaagtagatg aataactccg tcatcctggg cgaggaattc agcgtggcga acagctttgt
     3901 cgctcaattc catttcaaat acatcaactg gcataatgat tgtcttatac ataacccttt
     3961 ctccctgtta atcatgaaca aatcattcgc catgattata atatttatcc ctgatatttg
     4021 tctggttctt tttccttacg aacggtttct gtgattaata tattctcact gaacaccagg
     4081 aattctccca aaacctgcgg taccgcccgt tttcccgctg tgatagctac ccttaaagac
     4141 tgactctttt ttgaactgtc tctggaggtt gcatgaaagc attgacttat cacggcccac
     4201 atcacgttca ggtagaaaat gttcccgatc cgggcattga acaggctgat gatattattc
     4261 tgcgtattac ggcaacggcg atctgtggct ctgacctcca tctttatcga ggcaaaatac
     4321 cccaggttaa acatggcgat attttgggtc atgaatttat gggggaagtc gttgaaaccg
     4381 gaaaggacgt aaaaaatttg caaaaaggcg accgggtggt aattccgttt gtcattgctt
     4441 gtggcgactg ttttttctgt cgaatgcagc aatatgccgc ctgcgaaaat accaatgcgg
     4501 gtaaaggcgc tgcgctcaat aaaaaacaga taccagctcc cgcggcattg tttggttata
     4561 gtcacctgta tggtggcgtt cctggtggac aggcggaata tgtccgcgtc cctaaaggga
     4621 atgtggggcc gtttaaagta ccgcctttgc tttcagatga taaagccctt ttcctttctg
     4681 atattctgcc aacggcatgg caggcagcaa aaaatgcgca gatccaacaa ggttcaagcg
     4741 ttgcagtcta tggtgccggt ccagtgggat tgttgacaat cgcctgtgca cggttgctcg
     4801 gtgcggaaca gatttttgtt gttgatcatc atccctaccg cttgcgtttc gccgctgacc
     4861 gctacggcgc gatcccgatt aatttcgatg aagatagcga tccggcacag tcaattattg
     4921 aacaaacggc aggtcaccgg ggcgtggatg cagtaataga cgccgtcggt tttgaagcga
     4981 aaggcagcac cacggaaacg gtgctgacta acctgaaact ggagggcagc agcggtaaag
     5041 cgttgcgcca gtgtattgcg gcggtcaggc gtggcggcat tgttagcgta ccgggcgtct
     5101 acgctggatt tattcacggt ttcctgtttg gcgacgcctt tgataaaggg ttgacgttta
     5161 aaatgggaca gacccacgtt cacgcatggc tgggtgaatt attaccgtta attgagaaag
     5221 gattactgaa accagaagaa attgttaccc actatatgcc gtttgaagag gccgcccggg
     5281 gatatgagat tttcgaaaaa cgtgaagagg agtgccgtaa ggtgattctg gtacccggtg
     5341 cacaaagcgc agaggcggcg cagaaggcgg tttcaggtct ggtgaatgcg atgccggggg
     5401 gaacaatatg atcgtcagga gtggttttcg aggtaaagga cagccatgac gataatcgcc
     5461 gccataatca gaaatcctat caggatgtaa aatgcttctg ccatggttat tcccacaaac
     5521 gaaacggaat aattttgcag caaaagtgaa cagtgagaac caggaaaaat tgctgatttt
     5581 gcgtaaagag gatgcgagtg catcctctgg gcaaagcgag ttatcgcttg tgcagatggg
     5641 attaaagcag gtagtcgcca gcagcttctg gctggtattc gagttccagc acttcaaggt
     5701 gggttgcaac gccgcccgga agttcccagt gaatggaatc gccaacgcgc agccccagca
     5761 gtgcggcacc taccggagcc ataacggaaa gctgagtatt gctatcggtc atttttgccg
     5821 gatacaccag cgtgcgcaca cgcacttcgc catcgctaag attgcggaat ttaacccggc
     5881 tgttcattgt caccacgtcg tgtggcatct cttctggcga acacatttgg gcgcgatcca
     5941 actctgcgtt taacgcgtcg gcgattggca aaccagcata ggcgggttgc tccagcagaa
     6001 tatcgatgcg ttcggcatcc aggtcgttaa tgatgatagt tggtctggac atttttactc
     6061 catgtcgtcg gtactgcgag tgtcgcagat aaacataccc aaaagaaaac cctcaccgtc
     6121 aggcggcgag ggtttaactc acatgatgat actgactgtt gctcactctt tgaagtgatt
     6181 tgcgtcacat tcagggaatt cctcaatgca gcgcattatg tataattctt aatcgtcacg
     6241 gtttatggaa gacgaattag cgtgttttgt aaatcagatg attaataacc cactttatca
     6301 atcacaaagg ttttgccaca gttgcctggg tgtggttgtg gcaagaatga gtttgcagaa
     6361 agcggagcgt tgatggcgtc agctttgatc gagatctgaa tttcagtcaa atacgcaggg
     6421 ttaccctggc acgttagctt aactgttttc acgttctctt ttccccagct tttggcaaag
     6481 gcggcgtcaa agtcacggcg gctcactgtt ttaccgtaat tatccgcaag gaattttccg
     6541 gcttcgctct ctttaatttc ttgattcagg cgtaccatcg taccgaagta tgcgtccgga
     6601 tcaaaaccaa agcaggcacc gtgtttggca tattcgtagc gttccaggca ggaacgtccg
     6661 ccagctcctg gcatgacttc acttagttta gcggccgttt ccagtgataa cccggtttcc
     6721 ggcgatgaac acatccggct ggcgtgcgct tctggtagat tcgggattgg gcgagtagcg
     6781 caaccgaagc gcatccagcg gcgttcatca acaccacggg cagcaaccga tttaggcaat
     6841 cctggccaca gaccatgtac ggtcagaaaa tcagctttgt tggtcgtttc ggtttgtagg
     6901 cgacattcat ctcgttcgtt acgatttcga tcgtgttgac tctggcaaaa tccggtttgc
     6961 caggagaggg ccaggacata gcgatcaaaa tcgccatact gttttgcctg caacgctacg
     7021 gcgttggcag aagagaaggg aagcagagaa accgcgagca acgcggcgtt acgccagaat
     7081 gctttcataa tgggtgtgga actcatacat acactgaata ctatctatta aatcataaaa
     7141 aagcccgcca ggtgggctta cctggcgggc gtgatgattt attcagcgtt tggcgaacgt
     7201 attagttcca catggcgaga atcggccagc caaccaacag caacatagag atgtaaatca
     7261 ccccgaagat tgcgccaaga cgccagtaat cttttgattt cacatagcca cagccgtaaa
     7321 taatcacccc aggaccggtt gcatacggcg tcagacagcc catgataccg atagacagca
     7381 ccagcaggat acacagttgt tccattggta cgcccggaat acctttaccg acggccagaa
     7441 taaccgacag catggttgcg gtgtgcgcag acaggctggc aaacaggtag tgtgcaaagt
     7501 agaacaccag aaccagtaca atcaccgttg cgtttggtga gaatccttcc aggtgcgtac
     7561 tcatggtacc ggcgaaccag tcaataaagc cagaacgagt caggccgtta gccatcacaa
     7621 ccagagttgc caggttgacc agcgtgttcc atgcgctgtt atagcgggta atatctttcc
     7681 aaggcacaac gtgcagggcc agcattagcg aaactgccag cagaccaact gcagtagcat
     7741 taatgacttc actgccaaaa acccacaaac ctaagctgag caatacaagg ccaatcagtg
     7801 tccactctct gcgtgtcagc gcacccatgg ttttcagttc atcacccgcc caggttgcca
     7861 cttcttcact gtgtgtgatt tccggtttgt acagcacgta ggaaagccac ggcgcaatga
     7921 taagcaagat aaccccaacc ggcaggaagc agaggaacca ctgcaaccag ctaatctgga
     7981 taccggcaat tttgctgacg aactccagac ccagcacgtt tggtgccgca ccggtgacaa
     8041 acatggacga actcagactg gtactaatga ccatcatcca catcaaatag ccgccaatac
     8101 gacgcgcgga cggatcgttg gggaatgatt taaacagcgg cggcaggttt ttaatgaccg
     8161 gaaaaaccgt acccccggta cgcgcggtgt tggacggtgt aaacggtgcc agcagaatgt
     8221 cgataatgac aatcgcataa cccaacgtca gcgtgcgttt gcccatgaat ttcaccagga
     8281 aaagggcaat gcgacgacct aacccggaaa cttcataccc taatgcaaaa ataaatgcgc
     8341 caaataccag ccataccgtg gtgctggaaa aaccagccag accccatttc agcgcctgtt
     8401 tttgcgcatt aaacgctggg tcagctaatt ctttggcatc aaagagcagg taattactgc
     8461 caataacgca aatagtaacc gcaataaaac tgatcgctgt tgccggaatt ggctcgagga
     8521 tcatgccgac aatcattgcc acaaacacag cgaagtaatg ccatgcctgt ggtggcatac
     8581 cgtcggggac agggataaga aacatgacac ccatcaccac cagtggggcc aatagtttcc
     8641 atatattatc ttttgctaaa gacatacggg ttctccgaaa attaatattt ccaaatttat
     8701 caagtgctta aataattaaa tctgtgctaa aaactaggta aggatcagca ggtcggcact
     8761 gccgcctgga ctgagatttc gttcgataca ctccctgtcg aactgccgga gataatcgag
     8821 atcggcgggg gttcgaatgc cccctttttg caataatgtt tgcgcctcgc gctgtagcca
     8881 gcgcaggccc ccctcgccac cgcgcgatgc aacgttggta tcgccgttga tcgccatcag
     8941 taggagcaag gtatcgagca atgccagttc aggatctaac ccctgatcca gcagagtgag
     9001 gtaatgcggc aaggcgtgat tgatcaccag tggataaccc gcttcggctt caccgcgtgc
     9061 gccggtaagg ccaagctgct ggtacaaccg ttgacctgcc gtcagttgtg aattattggt
     9121 acgcagttct cgatcggtca ggccacggca gaaacttgcc gccgtagaac aaacggttgt
     9181 tggcgttacc ggttggttga gttgaagcaa acggccaatt gccgcacata gtagccctaa
     9241 agaaaaaatg ctgcctttat gcgtgtttac gcccgcagtg gcgcggaaca tatcaccttc
     9301 gcaagccata ccaattgggc gtaatccgtg gagtaccgct tctggtgcca tttccgcact
     9361 acaggcacca aattcaatga aacggggtag ccagccctga atcgccagcg cgctgcggtg
     9421 gaaatcttcc agcgccatat ctttatgtgc accgcagtta atccgatcca cgaggcctgg
     9481 tttcggtgac agattgactt cagtcagcat ggcgcgccag cccagcaggg cgtactcatc
     9541 gatcaatgac gtcgcaagct ttgtggtttt agttgacgtt gcaggcatcg acatcgttca
     9601 gcagtgcctc caggcggttg agtaaatcgg tcagttgatg ggtttttcca cgcgcgcaga
     9661 cggctgcgct ttgctcgcac aacaggcagc ggcgaggcgg cagtgaatag tcgcggcggg
     9721 agagaatttc gccttcgggc gtcaggacat cgatatccca taaccgcccg agaggatgac
     9781 tatgttcaag ctcaatggtg gcgagcttga ggtcgcgagc cggggcggca atgctcaaca
     9841 tgccctccgg cccgctggcg gaaaccagtg cagcctgctc ctgaatttgc cagccctgtt
     9901 ttgcggctaa ggtacgcaag gctgtcacgc catgattaaa aattcggcgt gtgacctcgc
     9961 tgtctttaat cggcccaggc gcaaccacgg taaaggagac cagtggaaca ggatggcgct
    10021 tgagccagac gtgttgccgt gcttgccttt catcccggct gacgagcagc tcgggaattg
    10081 acaccgcatg gtggctggcg agttcaggaa gcaggtgcat ggcttattcc ttcacctgat
    10141 gcacaacatc gatcaccgag ccatcgcggt aacgcacaac ggcaacgacg cggtcggtga
    10201 attcaatcgg ctgtggttca ccggtcagca gacgcgcgcg ttcgcgcagc cactcaatgg
    10261 aaaccacttt aatgcccgct tcctgcagac gttctgccag ttccggacgt gccgggttaa
    10321 ctgcgatacc gtggtctgtg accagaatat cgacactgga gcctggggtg atgcaggtca
    10381 gcacgttatc caccagagtc ggaatacgac cgcgtaccag cggcgcgacg atgatggaaa
    10441 gcgcagaggc aatcgcggta tcgcagtgac caccggaagc accacgcagt acgccgtcag
    10501 agccggtcag cacgttaacg ttgaactggg tgtcaatttc cagcgcgctc agtaccacca
    10561 cgtcgagacg atcaaccgat gcgcctttcg aaccccagtt agcgtactgg ttggcgctga
    10621 tttcgatgtg attggggtta cgggccagcg attgcgcagc atggctgtca aagctctgca
    10681 catccagcag tttgcggatc agaccttttt cgtgcagatc aaccatcgtc gcggtaatac
    10741 cgccgagggc gaagtcggcg cgaatatcgc ggctacgcat tttgtcttcc aggaaacggg
    10801 ttaccgccag cgatgcgccg ccggtgccgg tttgcatgga gaaaccttct ttgaagtagc
    10861 cagagttgac aatcacatcc gcagcgctac gggcaataag cagttcgcgc gggttagtgg
    10921 tcatacgggt cgcgccagcg ccgatttttg cagcatcgcc aacgcggtca actttgacga
    10981 tcaaatcaac ctgatcttgc tcaatgcttg ccggattatg cggataaggc agcagttctt
    11041 cggtaagcat cacaacctgt tttgcgttgt cggcatcaac cattgcatag ccgagggagc
    11101 cgcagcaggc tttaccggtg taaccgttgg cattaccgaa ttcatcacag gacgggacgc
    11161 cgaggaaagc cacgtcgata ttcagttcgc cgctctgtac cagatgcaca cgtccgccgt
    11221 gagagtggat ttgcaccggt tccgcaagca gaccacggga aatttcttct gccagtggac
    11281 cgcgcaggcc ggaggtataa atgcgggtaa ccacgccctg gcgaatgtgt tctaccagtg
    11341 gcgcatggca atcactcagg gagctggacg ccagggtcag gtttttaaag cccatcttag
    11401 cgatgacgtc catcaccata ttgacggtca ggtcaccgcc acggaaagcg tgatggaagg
    11461 aaaccgtcat gccgtcctgt aaaccagagc gacgaatcgc ttcttcctgg ttggcgcaca
    11521 gtttgcgatc gcgcgctttt tcagcctggt aggtttgctt tggtgagttc tggaaagcgg
    11581 caagatcgca ttcagcgcga cgattccagg ccgctacccg ttcttgtcgt tgagattgtt
    11641 caattttctg cgtcattttg attgccttat tcttcgcgga tgccggaaag ttctgcacgg
    11701 gagagcacca gacgggcacg atcgataacc ggaccgtcca ccatcttgcc gttcagggaa
    11761 accacgccga ggccttcgcg agcggcggct tcagcggctt ctacgacgcg gcgggcgtga
    11821 tccacttctt tctgggtcgg tgcgtagagg ttgtgcaaca gatcaatctg acgcgggttg
    11881 atcagcgatt tgccgtcaaa gcccagctgt ttgatgtggg cggcttcttg caggaatccg
    11941 gcttcgttgt tagcgtcgga atagacggta tcgaacgcct gaatacccgc agagcgcgcg
    12001 gcctgcaaaa tggaacagcg tgcgaacagc agttcagttc cttccgggga gcgttctgta
    12061 cgcaggttgc gcacatagtc ttctgcaccg agggcgatac cgatcaaacg ctcggaagcg
    12121 tgagcgattt ccactgcgcg ggtaatcccc agcggagatt caatcgccgc cagcaggccg
    12181 gtgctgccgg gttcacgacc acaggctttt tcgatacgca ggatctcttt ttcgatatcc
    12241 agaacatcct gagcggtatc ggttttcggc agacgcacaa cgtccgcacc accgcgaacg
    12301 acggcttcca ggtcgttgac accccattcg gaatccagcg cgttgacacg cacaatggtt
    12361 tcaatatcgc gatacagcgg atgttgcagc gcgtggtaaa ccatgcggcg ggcggtgtct
    12421 ttttcacgca atgctacgga gtcttcgagg tcaaacatca gggcatcagc cgggtagatg
    12481 aaggagttgc tgaccatcgc ggcattggca ccaggcacaa acaacatgct gcggcgggtg
    12541 cgagttttac gttgttgcag cgaagcggaa atcattggca atcctcccat ggcagagccg
    12601 ggataccgct ggcgcgtgcc agcagggctt ccagtcgtgc acgtaaaatg cagtccagtg
    12661 cgcctttgtc atcgacattc agctgtacgc cgcgcacgtt gtagcgggcg agaacgtcca
    12721 gaatggtggt gcgaattgca tcgccaaact gtttctcaac gctgctattg atttgcaggt
    12781 cgatatcctg cgtatcgagt ggggcgatgc gtatcatcac atccccagac tcaagggtgc
    12841 ctgcaacggc gggctggttt attttcattt ttcacctgtt tctcatgcgg gggtcttttg
    12901 acgagctgcc gcgtcctggc gggagtgctc aagcaggttc tgcaaataat gcagcgtgac
    12961 tgcagggacc agcggcgcga tagccgtgag atcgtttttc gccagcagtt gacgtacccg
    13021 ggaagcggat atcggcatct cctggtaacg cagccgctca atttcaacca gttcgatggg
    13081 cggtgcggag atagtcggcg tttccagcca gtagcgcata tcctggttgt actgggcggt
    13141 aacgcgacaa aagggttcag taccgacaaa gcggtgagtt acacccagcg cgggagcgag
    13201 gtactgacgg aaaatcttca gatcaatttc ggtgtaacaa tggttaatga cgctctgttc
    13261 tttaatgaag tagcaaggga acgtagcgcg ggagatgatg tattcggagc cgcgatgcac
    13321 agtcaggcgt ggaatatcgg cggtgccttt taacaccaga tccaaccggt cttcataggg
    13381 gaagcgtgaa gaatcttctt tgactaaaaa cagatgcaac cagtcgcact gtgccgcagc
    13441 ttgttgaatc agataacggt gaccattcgt gaagggattg gcgttcatca caatgcagcc
    13501 aatcttgttc cctggatgac gaaatttttt cagcgattcg gcatagcgtt tcagccgcgt
    13561 ggcgctgttt tccatcagca ccatcacgcc gggtacgctg gtcagcgtgg aaaaaccgca
    13621 ctggcggaac agcgcctcgt attcggttt
//
LOCUS       DAKISA010000110        13548 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.110, whole genome shotgun sequence.
ACCESSION   DAKISA010000110 DAKISA010000000
VERSION     DAKISA010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13548
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.110"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..312)
                     /locus_tag="OVW45_003451"
     CDS             complement(<1..312)
                     /locus_tag="OVW45_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCV5527814.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LA"
     gene            complement(331..2208)
                     /gene="bglF"
                     /locus_tag="OVW45_003452"
     CDS             complement(331..2208)
                     /gene="bglF"
                     /locus_tag="OVW45_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCV5527815.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     NTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMVATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDVTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="OVW45_003453"
     CDS             complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="OVW45_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCV5527816.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKCAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDYC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(3464..4189)
                     /gene="phoU"
                     /locus_tag="OVW45_003454"
     CDS             complement(3464..4189)
                     /gene="phoU"
                     /locus_tag="OVW45_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCV5527817.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4204..4977)
                     /gene="pstB"
                     /locus_tag="OVW45_003455"
     CDS             complement(4204..4977)
                     /gene="pstB"
                     /locus_tag="OVW45_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCV5527818.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5068..5958)
                     /gene="pstA"
                     /locus_tag="OVW45_003456"
     CDS             complement(5068..5958)
                     /gene="pstA"
                     /locus_tag="OVW45_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCV5527819.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(5958..6917)
                     /gene="pstC"
                     /locus_tag="OVW45_003457"
     CDS             complement(5958..6917)
                     /gene="pstC"
                     /locus_tag="OVW45_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCV5527820.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7004..8044)
                     /gene="pstS"
                     /locus_tag="OVW45_003458"
     CDS             complement(7004..8044)
                     /gene="pstS"
                     /locus_tag="OVW45_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCV5527821.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKEIGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDETIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8292..9364)
                     /locus_tag="OVW45_003459"
                     /pseudo
     CDS             complement(8292..9364)
                     /locus_tag="OVW45_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(9375..11897)
                     /locus_tag="OVW45_003460"
     CDS             complement(9375..11897)
                     /locus_tag="OVW45_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCV5527822.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSCVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLLGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            complement(11922..12653)
                     /locus_tag="OVW45_003461"
     CDS             complement(11922..12653)
                     /locus_tag="OVW45_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCV5527823.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEK
                     ILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRL
                     IPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            complement(12701..13273)
                     /locus_tag="OVW45_003462"
     CDS             complement(12701..13273)
                     /locus_tag="OVW45_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HCV5527824.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT         3599 a         3417 c         3035 g         3497 t
ORIGIN      
        1 cgctaacccc gcttcattcg gttcggcttc gtcgccctga gggaaaattc gcgcccaggc
       61 aatggaaata cgcagacagg tgaagcccat ctcggcaaat aacgcgatat cttccgggta
      121 acggtgataa aaatcgatgg cgacatcttt gatattctct ttccccagga tgcgcggttc
      181 catttttccc ataacgccat gaggctgtaa atctgaggtc gtgatccctt tgccatcttc
      241 cagccaggca ccttccacct gattggcagc tgttgcgcca ccccaaagaa atgtttctgg
      301 aaatgctttc ataattaact ccttttatcg ttagcgaatg atggataaca gcggttcacc
      361 tgcgcttatc tgcgccgtgc cgtggggtaa tacgtctgta aaatcatcgc tattactgat
      421 taataccggc gtcgtcacat caaatccggc ctcgcgaata gcagggatat caaaagaaat
      481 cagccgatcg cctgtattga ccttgtcacc cacgttgacg tgagcggaaa agaatttgcc
      541 gtccagtttt acggtgtcga taccgacatg aatcaggatc tccacaccat catctgactc
      601 aatgccaatg gcgtgtaatg tggcgaacaa cgaagcaatt cgacccgcaa ccggagaacg
      661 cacttcacca accgagggca gaatggcgat acctttaccc aacaggccac tggcaaacgt
      721 ggtatcagcg acgtgaatga gcggcacaat ctctcccgtc atcggtgaac agataccgcc
      781 ctgctcaggt ggtgtaataa cctctggtgt tttctcttgc ggggcaccct gcgctggctg
      841 acgtttagcg gtgatgaaat gaagcatcac cgtaccgaca aatgcgcaac cgatggcaat
      901 gacaccgcca ataacgctgg cccagacggt gaaatcaatt cccgttgacg ggatggtttg
      961 catgaaggtg aaaatacttg gcaaaccaaa ggagtacact ttcgtttgcg cgtagccaat
     1021 gatggtggcc cccaaagccc cactgataca ggcgataaca aaggggtact tacgcggcag
     1081 attaacgcca tataccgctg gttcggtgat accaaacagg cccgtcaacg ccgctgatcc
     1141 cgccaccact tttttctgcg catcgcgttc gcagaggaag acgccgagcg ccgccccgac
     1201 ctgcgccata atggcgggca ttaacagcgg gatcatggtg tcgtagccca gcacggtgaa
     1261 gttattgata cacagcggca ccaggcccca gtgcagtccg aacatgacga agatttgcca
     1321 gaagccgccc attaccgcgc ccgcaaatgc aggaaccgcc tgataaagcc agagataacc
     1381 ggcggcaatc agttcgctta tccaggttga tagcggcccc accagcagaa aggtgacggg
     1441 tgtgataacc atcagacata gcaatggtgt gaagaaattt ttgattgccg acggtaacca
     1501 cgcattaagt cggcgttcca gaatgctgca caaccaggca gaaaaaataa tgggaataac
     1561 cgatgacgag taattcaaca atgtgaccgg aatacccagg aaatccagcc ccagcgcatc
     1621 cgctttttgc ccgttctcga aagcagtcag aattaatgga tgcactaacg ctccaccaat
     1681 caccatggca gtaaatggat taccgccgaa gcgtttcccc gcggtgtatc ccaggattat
     1741 cgggaagaac caaaacaagg catcactggc gctgaataaa attaaataag taccactttg
     1801 ttcggtcgtc cactgaaaag tgagcgccag agccagcata cctttcagga tcccggttgc
     1861 caccatcaaa ccgatcagag gcgtaaaaat acctgaaata acataaacaa agcggtttag
     1921 cagattacct ttctcatcat tttccggtgc ctgttgcgct ttttcgccaa ggcctgccac
     1981 gctgttgacc gccaggaaga catcggccac atggttacct atgaccacct gaaactggcc
     2041 accgctttcc accaccataa taataccggg ggtgtttttc agtacctcag cttgcgcttt
     2101 gctttcatcc tttaacttaa aacgtaatcg cgttgcgcaa tgcatcagac taacaatgtt
     2161 atctgcgccc ccgactcctg cgactatttt tctggctaac tccgtcataa cttgccctct
     2221 accgctttgc ggcaaaactc caaaaaaaaa actgaaaaaa acggcctgac gtgaatcaag
     2281 caattttttt caggttttgc ccgcttagtg cggtaacaat cctttactca gtaataatat
     2341 ttcagtgttc tttgcgcacg cgctctatat ttatggctaa aaacataatc tctgcgggtg
     2401 aaattttacg ttgatactgc aaaccaataa aaatggcgat ccgttccgca cattgccatg
     2461 cttgcgggta attttgtttt actgcttgtt gtaatgattc atcactatcg ttaattgaag
     2521 catgttcaag aatacgccag gataaaaact tcagatgtgt aaccagtcgc tgataactca
     2581 agctttcttc ctggtaatta aggctgaact gaaattttat taattgcagc atttcgcgca
     2641 ttaactgcgt gacacctgca acatcctcca tatttccgct catttgggca ctgaccagat
     2701 gcatggcaat aaagcccact tcatctttcg gtaactgcac gcccaaccgt ttatcaataa
     2761 tggttaacgc ttcttcccct agctggaact ctttcgggta aagccgctgg atatcccaca
     2821 gcaacgggtt gggcagcaac acgttttgct gaaagcgttt aatcgcaaac tggcaatagt
     2881 cagttagcga gatataaata ctgtcctgta attttcccaa gcgctcctgc gctaaagaga
     2941 taatacgatc acaggttgcc atcacctcaa gaggaatatg acttaagagt tcgcttaatc
     3001 gcccgttcag ttcatgactg ctcaaggcat actctttttc tattccactt gagttaattc
     3061 tttcgccagc gcatttttga aagccaattc ctcgccccat gacgactttt tcccgctgtt
     3121 gatcatcaat aaccaccaca acattattgt tgagaatttt ggtgatttgc atgttcatag
     3181 caaggacctt ttttataaac aaaaaaaacc gacttcacca gtattctctg gttatgtcag
     3241 gttttgcctg cgaatgcagt aacaatccag tcatttatta atggttttta taacgaacat
     3301 ccaggttcgg aaattaattt aattgcgtgc agagaaaata gcaatgcgct attgataaaa
     3361 atatgaccat gctcgcagtt attaactttg tgtaatttta ggaatttata aagttatata
     3421 taacaaatcc caataattaa gttattggga tttgtctggt gaattatttg tcgctatctt
     3481 ttcccgccag cagtttatcc agctcatcac cgcctacatg acggaaatcc tgccccttca
     3541 cgtagtagaa gataaactca caaatattct ggcagcggtc gccaatacgt tcgatagaac
     3601 gcgcgcagaa cagcgcagta agtacgctcg gaatggtacg cgaatcttcc atcatgtagg
     3661 tcatcagttg acgaacaata ccttcgtatt cctgatcgac ttttttatct tcacgataaa
     3721 tacgtaccgc ttcgtcaatg tccatccgcg cgaacgcgtc cagcacgtcg tgcagcatct
     3781 ggatggtatg acggcccagc gactccagac ttaccagcaa cggctgatgc tgctgggaga
     3841 atttctccag cgcagtacgg cagattttgt ccgccacgtc gccaatacgc tccagctcgg
     3901 caatggtttt actgatcacc ataaccagtc gcaggtcgct cgccgtcggc tgacgtttgg
     3961 cgataatgcg cacgcaggct tcatcgatcg ccacttccat catgttgacg ttcttgtcgc
     4021 cttcgatcac gcgcttcgcc agatcgctgt cctggttatg catcgcggtg attgcatcag
     4081 aaagctgctg ctccaccatg ccgcccatgg tcatcacctg cgtgcggata ctttccagtt
     4141 cggcgttgaa ctggccggaa atatgtttat taagattgag actgtccata acgcactcct
     4201 gaatcaaccg taacgaccgg tgatgtagtc ttctgtttgt ttcttcgctg gcttggtgaa
     4261 cagatcgtcc gtgttgctga actcaatcaa ttcgcccagg tacataaacg ccgtgtggtc
     4321 ggaacaacgc gcagcctgct gcatgttgtg ggtaacgatc accacggtgt aatcctgctt
     4381 cagttcggtg atcagctctt caatacgccc ggtagagatg gggtcgagcg ccgaacacgg
     4441 ttcgtcgagc agcagcactt ccgggcgaat ggcgatacca cgcgcaatac acagacgctg
     4501 ttgctgacca ccagagagag agtaaccgct ctggtgcaat ttatctttgg tttcgttcca
     4561 caatgcggct ttggtcaatg cccactgcac gcgctcgtcc atgtcggcac gggagagctt
     4621 ctcaaacaga cgaacaccaa aagcgatgtt gtcgtagatg gacatcggga acggcgtcgg
     4681 tttctggaac accatgccca ctttcgcacg cagcagtgcg atatcctgag agttggtcag
     4741 gatgttgtcg ccatcaagca gaatttcacc ttctgcgcgc tgctccggat acagttcaaa
     4801 cattttgttg aaggtacgca gcagcgtcga tttaccgcag ccggacggcc cgataaacgc
     4861 cgttacctgg tttttagcga tatccaggtt gatgtttttc agggcatgga atttgccgta
     4921 gtagaagttc aaattacgaa cctgaatttt actcggggca gtttcaacca tactcattgc
     4981 aatcttttcc tcatcgtacc tgatgctttt cgctaatcag gcttaccgta ggccgggtaa
     5041 ggcgttcacg ccgcatccgg caaagtgtta accgtgttta ttcttcgcaa aaacaacgcg
     5101 cgccagaatg ttcagcagca ggacgcacag ggtaatgatc aataccccgg cccaggccag
     5161 ttgttgccat tccgcaaacg ggctcatcgc aaacttaaag atcgtcaccg gcaggttggc
     5221 gatcggctgc atcatgtccg tgctccagaa ctggttggag agcgcggtaa acagcagcgg
     5281 cgcggtttca ccggcaatac gggcaatcgc cagcaggata ccggtcataa tcccggacac
     5341 cgacgctttc agcgtaatcg cggagatcat cttccacttc ggtgtaccca gcgcataagc
     5401 cgcttcacgc aggctgtccg gcaccagttt cagcatgttc tcggtggtgc ggataacgat
     5461 cggcacctgc aacagcgcca gcgcaattac gcccgcccag ccggagaagt gctccatctg
     5521 tgccaccacg atggtgtaga caaacagacc aaccacaatc gacggcgcgg agagcagaat
     5581 gtcgttaatg aagcgaatca cttctgccag ccaggattta cgaccatatt ccgccagata
     5641 aatccccgcc ataatgccca gcggcgtacc gaataccgtg gcccacaaaa ttaacagccc
     5701 gctacccgcc agggcgttcg ccagaccacc accttccgta ttgggcggcg gcgtcatttc
     5761 agtgaacagc gccagcgaca taccgtcgat accgcgagtg atggtggaca ttaaaatcca
     5821 gatcagccag aacaggccga aggccatcgt cgccatcgag agcgtcagcg caatacggtt
     5881 tttgaggcgg cgacgcgcct gcattttgcg gcgagattca gccagcgccg cagtggtttg
     5941 catttcaacc atagccatta gcgtgccccc tcattcttag ccaggcgcat aatcataaac
     6001 ttcgatgcgg cgaggacgat gaaggtaatc acaaacagga tcaggcccag ttccatcagt
     6061 gcggcaacgt gcagaccgga ttccgcttcc gcaaattcgt tcgccaacgc agaggtgata
     6121 ctattgcccg gcatgtacag cgaggcgctg tcgagctggt aggtgttacc gatgataaag
     6181 gtcaccgcca tggtttcacc gagcgcgcgg cccagcccca gcatgatgcc gccgataaca
     6241 ccatttttgg tgaacggaag aacgatacgc cagataactt cccaggtggt gcagccaata
     6301 ccgtaggccg actctttcat catcaccggg gtttgttcga acacatcacg cattaccgcc
     6361 gcaatgtacg gaataatcat gatggcgagg atcacgcctg ccgcgaggat accgatacca
     6421 aatgcggggc cagagaacag cgcgccaaca atcgggatat tcgacatgat attgccgacc
     6481 ggctcctgaa agtaaacggc gaacagcggc gcaaagataa acaggcccca catgccgtaa
     6541 acgatacttg gaatggctgc cagcagctca atggcgatac ccagcgggcg tttcagccag
     6601 ccaggcgcaa gctcagtcag gaacagggcg ataccgaaac tcaccgggac ggcgatcagc
     6661 agcgcgataa acgaagtcac caacgtaccg tagatcggca ccagcgcccc gtagatatcg
     6721 ttcggtgcat cccactcttt ggtccatagg aaagccagac caaatttctg aatgctcggc
     6781 caggaggaga tgatcagaga gacaataatg ccacccaaca tcaatagcac aatcagcgcc
     6841 gccagtttta ccagcacgct gaaaattatg tcgccctttt tacccggtgg gttaaaagca
     6901 ggcttggttg cagccataag ttactcttca gttaaacgcg tttacaaagt cattttgtaa
     6961 tgccggatgc ggcgtaaaac accgtacccg gcctggagtt ttattagtac agcggcttac
     7021 cgctactgtc tttaatattg gtcttccacg cagcgcgaac ctgttcaact acactatccg
     7081 gcaggctggc gtaatccagg tcgttcgcct gtttagcccc ggttttgtac gcccagtcga
     7141 agaatttcag cacttctgtg ccttgttctg gtttcttctg atctttgtgg atcagaatga
     7201 acgtggtaga ggtaataggc catgcatctt cgcctttctg gttggtcaga tcctgagcga
     7261 aggttttgct ccagtctgca ccttttgctg cattagcgaa gttttcttcg gtcggactaa
     7321 ccggtttacc atcagcggag atcagtttgg tgtacgccag gttgttctgc ttcgcgtaag
     7381 catattcaac ataaccaatt gcacccggca gacgctgaac gaacgcggcg ataccgtcgt
     7441 tacctttacc gcccagaccg atcggccatt ttacggtaga gccagtacca acgttgtttt
     7501 tccactcttc gttcactttc gccaggtagc tggtgaagac gaaggaagtc ccggagccat
     7561 ctgcgcggcg tactacagca atgttttgtg aaggcagttt cagacccgga ttcagtttgg
     7621 cgatggtttc atcatcccac ttcttgattt tgcccaggta gatgtcgccg agggttttac
     7681 catccagcac cagttcgcca gacttcagcc ctggaatgtt aaccgccagc accacgccgc
     7741 caatcacggt cgggaactgg aacagacctt cctgagccag tttttcgtca gacagcggcg
     7801 cgtcagaggc accaaaatca acggtattag cgataatctg ttttacgcca ccggaagaac
     7861 cgataccctg gtagttaact ttattaccga tttctttctg gtaagtgtca gcccatttgg
     7921 catacaccgg cgcagggaag gttgcacctg cgcctgtcag gcttgcttct gcaaacacag
     7981 agaaagcgct catcgataag gtcgcggcga caacagttgc gacggtggta cgcataactt
     8041 tcataatgtc tcctgggagg attcataaag cattgtttgt tggctacgag aagcaaaata
     8101 ggacaaacag gtgacagtta tatgtaagga atatgacatt tttatgacag aaaggaatta
     8161 acaaataact tactgatttt aaatgtgaag atgaaaatcc cacatgtcat ctttttgtca
     8221 tgatgcattt aaaagtgata aagaaatggg tgagatgaca acgatgtgat gaacaatcgt
     8281 tgtcagacct attagcgcac aatctcaaca gttatcgtcg ctgttgcagt aaattgcccc
     8341 ggggctgggc gagctccagt aaggctcgca ggagcagctg aaaaagttac actacctgat
     8401 ttttcaccat acactgttga tttacccatg tctgttggca actcaccacc gttcactttg
     8461 atttccttat tatttttgtc gtaaattttt atccccacgt tagagtttga agttacgacc
     8521 attgaattgt ccgtattggg ttcggtagca taaaaagacg tactgatatc atcggtattc
     8581 agtatcccca ggttttcaca ctcaatagtg actgttttag taattttatg tgttgttacc
     8641 gcacttccaa caacagatga aaaatctgca gctggtattt ctttaaaatc aaaataaact
     8701 ggctgtaaat tatttatttc gcaactttgc ggagctgtaa tatcgccccg aatataaaca
     8761 tcagccatcg gctgtgagta atccaccggg gtgtcacggc taattgttcc atacaatgtc
     8821 actatctttg tttcaggaat aatcaatttt cctgcaaatg tcttcttgat ataaaatata
     8881 ctatcgcttt acccccactg gatatactta aaggttcaat gcggttgcaa ttgtactttg
     8941 tattaccaga cgggttgtta gcatgatatt caaaaggtgc attcacatac ccaaccccaa
     9001 gaatatctat agcaatccca acatcaaggt tcggattaag atcataataa ttaatattag
     9061 agtgaacccc agcataagtt aatgcctggt ttatttttga cgtataatag atatagggga
     9121 attctcctac attaccagta tcacaatgac agcgtccagg atatgaggat ccattagtta
     9181 cagtggtatt aaatgtcgtt ccaaccttat tttcgttcgc ggtaataaga tatcccgaga
     9241 aatcaagcgt acggtgaaat acgccattat caggggtaca gataccttta ggagctgcaa
     9301 gtgctacact acttaacgag agcataaaca cagcaaagca ccactgtttg atatacttgt
     9361 tcataaaaat ccttttaatg acatgtcaca ccctgttcaa ttaatccgct ctcactttct
     9421 ggtgtcgaaa gtgaataatt gaagatgcat tttgttgagg cgttttctcc ccaggataac
     9481 gtaacctgtc ctttttctgg tagaccagaa agataaagtt cgccacggtc accgacaatt
     9541 cctgaatctg ccaaacttgc atcttgtatc gcagcaatgg ctccaaaagg taccggttta
     9601 ccatttgcat ttaacactct gaagagaacg cgataaccca catgagtcac aaactccgct
     9661 ttaaccactg caccacgcgt gggaaccatc gtaacgacgg tattatcaag ttctatattc
     9721 gacttcgaga aatagttatc gctgattgct acccgatttt catcataagg gtttagctgt
     9781 gtcttgaccg tatagccgcg ccagtccgtc gcagcaccgc gcatattatc tatttctaac
     9841 ccggcggccc caggtgcttt aacaagagca atagtttcac ctaactcctg acttaacgtt
     9901 accccttcgc tgtgtgccag aattccccct ctgataccat aattcagcct ctgggaattt
     9961 tggctgtagt tatagctgcc gttcaccgtg ccataagagc catcataggt cagggacata
    10021 ttaccgccaa cctcatcatc atgactgtta taggactgca ttaaattcca tgaaaggtta
    10081 tttctttcca gcgccgtacc gctgagtccg agttgatgtg agctaccgga attatcgttt
    10141 tgcgaaacag aataggtggc gtaactatta ccccaccagc ccaataaagg aacagataaa
    10201 gagagcatgt agatctgctc actggtgtta tcactggcat ggttgttccc cgtcccctgt
    10261 ctggcagtat tggaataact ccaggagacg ttccacgaga gacgcttcca gctactgctg
    10321 tagccaaatt gcattaatcg ttcgacatca tcggtatgcc agtattgttg ccggcttaat
    10381 gtcaggtata aattgccata atctctcccc aacgactgcg ttagcgtccc ctccacgcaa
    10441 ctacgtcggt tacctgtttc ccaaaagtct tcagggctgt tgcgccgcga tgcccactca
    10501 ttgagggtat agtagccctc ggtggagtaa cgatagccaa ccaggcgcaa gctagtatct
    10561 gtgtcgtcaa atcgttttga atagagaaag cgatatgact gacccgtttc tgtatcatca
    10621 tggctaaaat tcgcacgagc atgtgtcaca tcgaaagaaa ttgcgcccaa agcgccaaga
    10681 tctttaccga cacccagagc tgcagaacca taattttcag cagcctgcat acctccatat
    10741 aaagtgatcc cgtgggaaaa accatgaagt atttgcgcct gaaggacgtc cggtgtaaat
    10801 ccgtcttcat cgcgcaattc acccacgctg acatcaacat ctgtctgacc ttcacgttta
    10861 agaatcgcta atgaagcata aggttgggtg aatcgttgtt cgctaccgtc ttcttctttg
    10921 atggttacgt ccaaatcgcc acctgtactg gcggtgttga gatcggtgat ctcaaaagca
    10981 ccttgtggaa cggcagactg ataaatcacg tagccattct gccgaatgat gacggtggcg
    11041 ttggactttg caatcccacg aaccacagga gcaaagccat tctggctggc gggtagcata
    11101 tcgttatcag tatacaaacg cgcgccgcga atttgcgtac tgtcgaaaat atcgctcgcc
    11161 gtccacgtat caccaatcat aatctggctt cttaacgcgg caatatcgcg ctgtatccag
    11221 gtactaatgg agttgtattc cgcacggcca tcgctggtgt ttaaagtact gtaattacgt
    11281 agccgccacg gtccaatatt cagtccattg cgcagattga gataactggt atctgtctct
    11341 tgtgaaccat aatctgaacg tagtgtatgt gaaccagaaa aatcgtagtt gatggtgagc
    11401 gcgttaatcc cctcatccca acgcgtgggg ctgatgtaat cataagccat cgcgtccatg
    11461 tagatttggg gaatggatat attcagctgc aatttatcac tgtcaaactg ccaggatgag
    11521 ccttcaatac gtggagcagg atcgaggcaa accgtctcat ccacatcatt gaacgatttg
    11581 agcgcatcaa cttttatgcc aaaggaggac agtgtttttt taatggcaat aaagcaagcg
    11641 tgcagatcag catctggatt atcctgaaca aatgtcacat cctgacgttc aaacatttta
    11701 ccgttgataa aaatatcaac gctataaaca cctgggggca tcaggtttgc ccgattaaac
    11761 tggcgaatat cgactccgga ttgatggtcg atttccagaa gcgccggatt gaatacactg
    11821 acctcggcca tcaggttttg cgagaaaatc agacacgtcc ctgccgtgag gccaaccact
    11881 attctggtcg tcatcataat acgcctatcc ttatcccttg cttacagcgt catttttatc
    11941 gggtcactga ctgcgccgta atcattaata aatcgccagg tcatttggcc tcccgcagaa
    12001 ttagcaggta aaggtaacgt gagcgtttca aaaggacgga tcatattgag gggcacatca
    12061 tggccatcaa cgatgacatc catcacactg atatgaattg ggttgggatt ctctgctatc
    12121 agatttcgcc cttcccggcg ccatttcact ttcggcgcag catcttcggg aataaggcgg
    12181 atatttgctg gtcgccagaa cagcttgata cgcgttttta ctgctatttc cagcgagttc
    12241 gtcgctttgt tgctcgctga tggcggaata tttttaatat tcagccagaa gactgactcg
    12301 cgatcatcag gcaatgacac acctttggtg tgaataatgc gtaatatttt ttcttgcgaa
    12361 ggttctaatc gtgatactgg cggaataacg gtaaacggcg ctttgctttt atcctcttta
    12421 ttaaatggat ctacccaagc ctggatgaga taaggtattt tttcttcttt gctaaataca
    12481 gaaatagatt tatcagcatt attacttaag taaaccaccc gcgtaccacc aatgacaacg
    12541 cctgcataag ttaatgctgg ttggcctaaa aaaacgctga gtataatcgc ggtaaataac
    12601 ttacatgaaa ctgttgtttt aaagagcctg attaaacaac tttttctgga cataacgact
    12661 ccctggccaa agccaactga ataaaaaggc ccttgataaa ttatttctta tattcgacag
    12721 taaattgtga atcagcgttc gccgtaccag ccgttacggt cgcattggtt gccacatagc
    12781 gtgcaataaa cttcatttca gccttgcctt cagcatcgat agcggtataa gcagagtcgt
    12841 tataaagttt aacctgcgaa ttatcggact ggttaaaaat acgaatacca acgccggttg
    12901 ccgcaatcgc ttgtcctgta ccggtaaaat caccagggtt gcctgctgta agcggcgtac
    12961 ccactttcag gtcgccgtcg ccgccaggcg cttctgtacc atcaaaacga acggctgctt
    13021 gagtgtacgt tgccgggcaa ttttcaagtt taatactgaa cttctgtggc gacgccagac
    13081 tacctacacc tgaaaatgca tttttactga ctttacccag tgggacaatt tgatcggctg
    13141 tatcggaggt aacttcacaa gtagtgtcga caatttcacc gacgaagtga acaacaccat
    13201 cttctgccag tgcatgcccg gccagcatta gagatgccag agtgaatgca ccgcctataa
    13261 tattacgctt cataaaaact ccattgatat aatatatcct ttaccatgaa tcatcactta
    13321 tattaataag tgtatttttg attcaaagga atagtacacc aaagagacgg ctttatctaa
    13381 aaataatatt aattacgcaa aaataaaata taaatttatt gtttaaatat tatttaaaca
    13441 gtaaaaaatc aagtttgtta tcagacatta atgataattc agaatatatt tatatagaaa
    13501 gatagatgaa tttattaatg tcagttataa aaaagggcca ccggtcgg
//
LOCUS       DAKISA010000111        13512 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.111, whole genome shotgun sequence.
ACCESSION   DAKISA010000111 DAKISA010000000
VERSION     DAKISA010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13512
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.111"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(146..3367)
                     /gene="carB"
                     /locus_tag="OVW45_003463"
     CDS             complement(146..3367)
                     /gene="carB"
                     /locus_tag="OVW45_003463"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCV5527825.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLTGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(3385..4533)
                     /gene="carA"
                     /locus_tag="OVW45_003464"
     CDS             complement(3385..4533)
                     /gene="carA"
                     /locus_tag="OVW45_003464"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCV5527826.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            complement(4989..5810)
                     /gene="dapB"
                     /locus_tag="OVW45_003465"
     CDS             complement(4989..5810)
                     /gene="dapB"
                     /locus_tag="OVW45_003465"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCV5527827.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLNLNNL"
     gene            complement(5977..6891)
                     /gene="rihC"
                     /locus_tag="OVW45_003466"
     CDS             complement(5977..6891)
                     /gene="rihC"
                     /locus_tag="OVW45_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCV5527828.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPIGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSSGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            complement(6957..7907)
                     /gene="ispH"
                     /locus_tag="OVW45_003467"
                     /gene_synonym="lytB"
     CDS             complement(6957..7907)
                     /gene="ispH"
                     /locus_tag="OVW45_003467"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCV5527829.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(7909..8358)
                     /gene="fkpB"
                     /locus_tag="OVW45_003468"
     CDS             complement(7909..8358)
                     /gene="fkpB"
                     /locus_tag="OVW45_003468"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCV5527830.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(8483..8977)
                     /gene="lspA"
                     /locus_tag="OVW45_003469"
     CDS             complement(8483..8977)
                     /gene="lspA"
                     /locus_tag="OVW45_003469"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCV5527831.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            complement(8977..11793)
                     /gene="ileS"
                     /locus_tag="OVW45_003470"
     CDS             complement(8977..11793)
                     /gene="ileS"
                     /locus_tag="OVW45_003470"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCV5527832.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(11836..12777)
                     /gene="ribF"
                     /locus_tag="OVW45_003471"
     CDS             complement(11836..12777)
                     /gene="ribF"
                     /locus_tag="OVW45_003471"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCV5527833.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(12785..13003)
                     /locus_tag="OVW45_003472"
     CDS             complement(12785..13003)
                     /locus_tag="OVW45_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCV5527834.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            13106..13369
                     /gene="rpsT"
                     /locus_tag="OVW45_003473"
     CDS             13106..13369
                     /gene="rpsT"
                     /locus_tag="OVW45_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCV5527835.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT         3165 a         3806 c         3400 g         3141 t
ORIGIN      
        1 tgtgagctaa atcctgctta tgtcatcaaa aatatcgatt ccccaaaaga tttacttaat
       61 gggcaaaaaa aacggtcctc atcagagaac cgtattactt attcgatcaa attagcggat
      121 gaaaaatatc cgccatgaca cgctattatt tgatctgcgc gtgcatttcc tgcaccgaaa
      181 ttactttttc ggtcgcgtcg gcattcagcg ccatcgcggt agcgaaacca ccgttcaggg
      241 tggtgtcata atgcacttta tattgcagcg cactgcgacg gatcacgcgg gagtcttcaa
      301 tcgcgcgacg gcctgaagtg gtgttgatga tgtaggtata ttcgccattc ttgatgcggt
      361 cctgaatgtg cggacggcct tcatgcacct tgttcaccag acgcggatta atgcccgctt
      421 cgcccagcac aatcgccgtg ccgtgggtcg catccagctc gaagccctgt ttcagcagtt
      481 ttgccgccag gtccaccacg cgttctttat cgccttcgcg cacggaaagc aacgcacgac
      541 cgtgtttctt catggtggag ttgctgccca gctgcgcttt ggcaaacgct tcagcgaagg
      601 tgcggcccac gcccatcact tccccggtgg agcgcatttc tggccctaac agcgggtcaa
      661 cacccgggaa tttattgaac ggcagcacca cttctttcac cgagtagtac ggcgggataa
      721 cttctttggt tacgccctgc tcagccagcg atttgccagc catcacacgc gccgccactt
      781 ttgccagcgg tacgccggtg gctttggaga cgaacggaac ggtacgcgcc gcacgcgggt
      841 taacttcaat caggtagact tcgttgtttt tcaccgcaaa ctgcacgttc atcagaccgc
      901 gcacctgcaa ttcgaaggcc agtttctgca cctgctggcg catcacatcc tgaatttcct
      961 gacttaaggt gtaggctggc agagaacatg cggagtcacc ggagtgcacg cccgcctgct
     1021 cgatgtgctc catgatgccg ccaatcagca ccatttcgcc gtcgcagatg gcatccacgt
     1081 caacttctac cgcgtcatca aggaagtgat ccagcaacac tggcgcatcg ttagacacgc
     1141 tgaccgccgt ctggaagtag cgacgcaggt cagcttcgtc atagacgatt tccatcgccc
     1201 gaccgccgag aacgtaagac ggacgtacca ccagcgggta gccaatctct ttcgccttct
     1261 ctaccgccat ttcaatagcg gtaacggtgg cgttcgccgg ttgtttcagt ttcagacgct
     1321 caaccgcatg ctggaagcgt tcacggtctt ctgcacggtc gatagcatcc gggctggtgc
     1381 cgataaccgg tacgccagca gcttccagcg cgcgcgccag tttcagcggg gtctgaccgc
     1441 cgtactgaac gataacgcct ttcggcttct caatacgcac gatttccagc acatcttcca
     1501 gagttaccgg ctcgaagtag aggcgatcgg aagtgtcgta gtcggtggag acggtttccg
     1561 ggttacagtt aaccataatg gtttcgtaac cgtcttcgcg cagcgccagc gaggcgtgta
     1621 cacagcagta gtcgaattcg ataccctgac cgatacggtt cgggccgccg ccgagaacca
     1681 tgattttttc acggtcggta gacggattcg cttcgcactc ttcttcataa gtggagtaca
     1741 tgtaagcggt gtcggtggcg aactctgccg cacaggtatc cacgcgctta taaaccgggt
     1801 gcaggtcata ctggtcgcgc agcttgcgga tttccgcttc gcgcacgccc gtcagttttg
     1861 ccagacgcgc atcggcaaag cctttgcgtt tcagctggcg caggaattca gcgttcaggc
     1921 cagtgatgcc cacttccgct actttctctt ccagacgcac cagttcttca atctgtacca
     1981 ggaaccagcg gtcaatattg gtcaggttga acacgccgtc cacagacagg cccgcacgga
     2041 aggcatcggc gatgtaccag atacgctctg cgccagcatc tttcagttcg cgacggattt
     2101 tggttaacgc ttccgggtca tcgaggctca ctttcgggtc gaatccagtc gcaccgactt
     2161 ccaggccgcg cagcgctttt tgcagggatt cctgctgcgt gcgaccaatc gccatcactt
     2221 cgccaaccga tttcatctga gtggtcagac ggtcgttagc accagcgaat ttttcgaagt
     2281 tgaagcgagg aattttagta accacatagt cgatggacgg ctcgaaggag gccggagtac
     2341 gtccgccagt gatgtcgttc atcagttcgt cgagggtgta acccaccgcc agtttcgccg
     2401 ccactttagc aatcgggaaa ccggtcgctt tcgacgccag cgccgaagaa cgggacacgc
     2461 gtgggttcat ttcgataaca atcaggcgac cgtttttcgg gttcaccgca aactggacat
     2521 tggaaccacc ggtttcaacg ccgatttcac gcagcaccgc catcgaggcg ttacgcatga
     2581 tttgatattc tttgtcggtc agcgtttggg ctggcgcgac agtgatggag tcaccggtat
     2641 ggatgcccat cgcatcaaag ttttcgatag agcagacgat gatgcagttg tcgtttttat
     2701 cacgcaccac ttccatctcg tactctttcc agccgatcag cgactcatca atcagcagct
     2761 ctttggttgg ggagagatcc agaccgcggg cgcaaatttc ttcaaactct tcgcggttat
     2821 aagcgatacc gccgccgcta ccgcccatgg taaaggatgg gcgaataatg cacgggaagc
     2881 ccacgtcagc ggcaaccgcc agcgcttctt ccatcgtgtg tgcgataccg gaacgcgcgg
     2941 tttccagacc aattttcttc atcgctacgt cgaaacgacg gcggtcttct gctttatcaa
     3001 tcgcatcggc ggtcgcacca atcatagtca cgccgaactc ttccaacacg ccctgacgct
     3061 ccagctccag cgcgcagttc agcgccgtct gaccgcccat cgttggcagc accgcgtccg
     3121 ggcgctcttt ttcaataatc ttgcgtacta cttcccagtg aatcggctcg atgtaggtcg
     3181 catcggccat ttccgggtcg gtcatgatgg tcgccgggtt ggagttcacc agaatgacgc
     3241 ggtaaccctc ttcgcgcagg gctttacatg cttgcgcgcc agagtagtca aactcacacg
     3301 cctgaccgat aacaatcggg cccgcaccca gaatcaggat actttttata tctgtacgtt
     3361 ttggcatggc tcttttactc ctgattactt agcggtttta cggtactgct caattaactc
     3421 gataaagtgg tcgaacaacg gcgctgcgtc gtgtggccca gggctggctt ccgggtgacc
     3481 ctggaagctg aacgccggtt tatcagtgcg atgaatgccc tgtaacgtac cgtcgaacag
     3541 ggatttatgc gtgacacgca ggtttgcagg taatgttgct tcatccaccg caaaaccgtg
     3601 gttctgggcg gtgatcatca ccacgttttt ctccacatct ttaaccggat ggttgccgcc
     3661 gtggtgacca aatttcattt tgacagtctt cgcaccgctc gccagcgcca gcagctgatg
     3721 accgaggcag atgccgaata ccggaatatc ggtttcgagg aatttctgga tggcggtaat
     3781 ggcgtaatcg cacggggccg ggtcgccagg accgttggag aggaagatgc cgtctggatt
     3841 cattttcagc acatcttccg cagaagtttg cgccggaacg atggtcaggc gacagcctct
     3901 atccaccagc atccgcagga tgttgcgctt ggcaccaaaa tcataagcca cgacgtggaa
     3961 cggcagctcg tcttcttttt tcgcttctgg caggccgccg gtcaacgtcc agctcccttg
     4021 tgtccagcta taggcttctg cggtggtcac ttcttttgcc agatccatgc cattcagacc
     4081 tgggaacgcg cgggcttttt ctaacgccaa cgccgcatcc gggttatcgc ctgcgataat
     4141 gcagccattc tgtgcgcctt tctcgcgcag taaacgcgtc agcttacggg tatcgatatc
     4201 ggcaatcgcc acgatgttat ggcgcttcag gtaagaagag aggtcttcgg tattacggaa
     4261 gttgctggca atcagcggca gatcgcgaat caccagacct tgtgcatgta cctgagaaga
     4321 ttcttcatcg gcgtcattag tgccgacatt gccaatatgg ggataagtaa gagtaacgat
     4381 ttgacgagaa taggaaggat cagtgaggat ttcttgataa ccggtcattg aagtattgaa
     4441 aacgacttcc ccaaccgccg aacctgttgc ccctatggcc cgaccgtgaa actgggttcc
     4501 gtcttccaga accaatagcg ctgacttaat caaaacaccc tccagagaat attcactcac
     4561 tttatttgca tattaattca caatgatgac gtaaatcaat gcaaatctgc ttaccgacga
     4621 atttctggca aacggcggca ttctggagat atgggcgcta aaagtcaact taatggcggt
     4681 attttttata ttattttgtg gctcatgcat gttttacagg cattaagcga caaaaagatc
     4741 atataacctg ttctggaaaa cgcttgcgca gcaagaagat tgcatgaaat aaaaacgagc
     4801 aggaaaaagt ggaccaaatg gtcaaaattt acattaaaac aacaaaaaca gaagacaaaa
     4861 catgtaattt ttggcactta tagaatattt aaaaacataa cactaaaaat aaaagggcca
     4921 aaaattaaag ccctttataa tcaataaatt aaatgtgtta tttttgcacc ataacaaata
     4981 ttttgtggtt acaaattatt gagattaagt acatctcgca tatcaaaaag accattttcc
     5041 ttaccactca accacaaagc cgatcttacc gcgccgttag caaatgtcat acggctggac
     5101 gccttatggg tgatctccag acgctcgcca atatcggcaa acatcgcggt atgttcacca
     5161 acgatgtcac ctgcacgcac ggtggcaaaa ccaatggtgc caggcacacg ttcaccggtg
     5221 tggccttcac gactgtagac cgcgcaatct ttcagatctt tatcaagggc gtgggcgatc
     5281 gcctctccca ttgccagtgc ggtgcctgac ggcgcatcaa ctttatgtct atgatgtgct
     5341 tcaataattt cgatatcggt gtagtcaccc atcactttgg ctgctttctc cagcagctta
     5401 agcatgacgt taacgccaac gctaaaattg gcggcaaaga caatcgcaat atcggcggcg
     5461 gcgtcacgaa tcgcctgttt accggcttcg tcaaaccccg tagtgccgat caccatccct
     5521 ttgccatgct ggcgacaaaa agcgagatgg ttcagcgtac cttccggacg ggtaaaatcg
     5581 ataaacacat caaaatcatc ttttaccgca tcgaggctgc tttgcacggt aacgcctgtt
     5641 ttccctgcac cagccagctc accggcgtcg ctgcccagta aagaagatcc ttcgcgctcc
     5701 agcgccgcgc ccagctgcac gccttctaat gccagcgccg cctgaatcaa ctggcggccc
     5761 atacgccccc cggctcccgc gatggcaacg cggatgtttg catcatgcat agctattctc
     5821 ttttgttaat ttgcatagac cgttttcaga gtaaccagcg catcgctacg ccgccagcca
     5881 aaaacgctga taattagaaa ttaatgataa acagggaggg atatcagtga aaggcatgag
     5941 tcgatgaatg actgcatgcc gataacatgt gacaggttac gacgccagag ccagcacctc
     6001 agccacccac tgctggaagc ctttcacatc cagatccaat gccaccttta cattggctgg
     6061 tttgcccagg caaccgtcga tatcaaccac cgtcgtgcct gaggtaaatt cgccttgagt
     6121 ttctaccgcg acaaaacacg gcttgagggt gaataagtcc gggcgcacca gccaggcgat
     6181 ggcgcagaga tcgtgcattc gcagaccgct ttgcatactg ccgctacggt agtggctaaa
     6241 cagtgcatga agcatttttc cggtgcgatt caactccggc aaagtagcga gatagtcagg
     6301 ggttaatatt gcctgattgg tgacatccag accgcacatg acgatttcga ttccgctacg
     6361 gaagacacag gcagccgctt ctggatcggc agcgatatta aactcggcat ttggtgtaca
     6421 gttgccgcga ccagaagaac cgcccatgat caccagacga cgaatatacg gtttacattc
     6481 cgggcattgt gaaagtaaca gcgcaatatt ggttaacggg ccaatggcca ccagggtaac
     6541 aggctctggt gcacgcatca gggcatcacg aatcgccaga aacgctggta tcccgattgg
     6601 ctttcggttg tgctcaacaa agtcgtagcc agccattccc gattcgccgt gcacagatgc
     6661 tgcatcacgc ggtgcgcgta ccagcggcac agcggctccc tgggcgagcg gaatctccgc
     6721 attccagaaa tgcagcagtt gcagggcgtt gcgggtggtt ttctcaaccg agacattacc
     6781 cgcgacggtg gtcatcagtt gcaggtcgag ttcgggggca aaaatcgcgg cggcaatggc
     6841 gacggcatcg tcaatgccgg ggtcagtatc gaggaagata ggtaaacgca tgttttctcc
     6901 ataaaaaatg ccggtaacaa gaccggcatt ttcgcataac ttaggctgct aatgacttaa
     6961 tcgacttcac gaatatcgac acgcagctct ttcggcactt cgaaaacaat gttttcttca
     7021 cggccttcca gcggaatggc ttcaccaccg cccagctgct gcaaacgtgc caccacattt
     7081 tgcaccagaa tatccggagc cgatgcgccc gcagtcacgc cgacgcattt aacctctttc
     7141 acccactctt cctggatatc tttcgcatcg tcaatcaaaa acgcgcgttt gcccatacgc
     7201 tgggccagct ccgccagacg gttggagttg gaggagtttt tcgaaccgac caccaacaca
     7261 acttccgcct gttctgccag ggcgcgtacc gcttcctgac ggttagtcgt ggcgtagcag
     7321 atgtcatctt tgcgcggacc gacaattttc gggaagcgtt tacgcagcgc gtcgatcaca
     7381 tcagacgtgt catccaccga cagcgtggtc tgggtcataa aggagagctt ctcttcgttt
     7441 ttgaccgtca gtttccacac atcgtccggc gattcgacca gatacattcc cccttccggg
     7501 ttactgtact ggcccattgt accttccact tccggatgcc cggcgtggcc gatgagaatc
     7561 gactcttcgc cacgacggct ggcgcgggcg acttccatat gcactttggt caccagcgga
     7621 caggtggcat caaacaccgt caaatcgcgg ctttttgctt cgttacgtac cgcctgagaa
     7681 acaccgtgtg cggagaaaat caggatcgcg ccgtccggta cttcgctaat ctgctcaata
     7741 aagatagccc cacgctcacg caagctatcg accacatagc ggttatgtac cacttcgtga
     7801 cggacatata tcggtgcgcc gtaaatggcc agcgcgtttt caacaatgct gatagcgcgg
     7861 tctaccccgg cacaaaaacc acgcgggttg gccaacagga tctgcatgtt acgcctccaa
     7921 tgccggatcg atttccagca cttcaatatc aaaatgaacg gtctgcccgg ccagcggatg
     7981 gttgaaatca acggtaatgg agtcgccgtt aatttcgcgg atcacgccag gcatctcact
     8041 gccatccatt gcggtaaaaa gcatgattgc gccaatttct ggctcgcctg catccataaa
     8101 ttcacggcgg gagaagtact gaatcaggtc cggtgacggc acgccaaacg ccgcatctgg
     8161 ctccaacgag aaggtggttt tatcgcccac tttcagcccc aacaggtgtt gctccagccc
     8221 ttcagaaaga gaagcatcac ccaggcggaa cagcgccggt ttaccgttgt tgcgggtaga
     8281 ctcggcggtg gtgccatcgt cgagttttag cgtgaagtgc accaggacgg cgctattgct
     8341 ctgtacagat tcagacatgc aggtttgctc ttatatttga ggatttttgc cggatgcggc
     8401 gtaaacgcct tatccggcct acgatttcgc agcaatctgt aggccggata agatgcgtca
     8461 gcatcgcatc cggcagggtt tattattgtt ttttcgctct agaaggcaaa aaaccttcca
     8521 gcacaatcag tgccgcaccg acacagatgg cagtatcggc aaggttgaag gtggcgaagt
     8581 gccagtcgcc gacgtagaag tcgatcatat cgacaacgaa gccgtgccac aggcggtcga
     8641 acaggttgcc cagcgcgccg ccaataatca gcgcgtaagc gatattgttt agcttctgcg
     8701 tggccttcga gcgatacatc atcactgcca ggatcacgct aataccaatc gcaataccgg
     8761 caaagaacca acgctgccag ccgccgctat cggcaaggaa actaaacgcc gcgccatagt
     8821 tacgcgcata atgcagatta agcgacggga acagcgggac cgtatccccc agagcaaagt
     8881 tctggaggat caggtatttg ctgcccagat cgataatcag cacgactacc accagccaca
     8941 gccagcgtag ccctgttgaa cagatcgatt gactcatcag gcaaacttac gtttttcacc
     9001 gtcaccggcg acgttgctga cacagcggcc gcagatttct gcgtgttccg ccaccttgcc
     9061 gacatcctgg gtgtagtgcc agcagcgtgg gcacttctca ccttcggctt tactcaacgc
     9121 gactttcagc cctttgagta cttcgctctg ctgagcatca gcaggtgcgt cgttatagtc
     9181 tgcaacggta gcgccggagg tcaacaggac aaatcgtaat tcatcgccca gcgcggtcag
     9241 tttcgccgac agttccggtt ctgcatacaa ggttactgcc gcttccagcg agccacccac
     9301 tttcttgtcg gcacgcgctt gctcaatgac tttgttcact tcgccacgca ctttcaacag
     9361 ctcgtcccag aacgcatcgt tcatcgcttc actgtctgcc aggccaaaca ggccttcgta
     9421 ccactcaccg gtgaagacgt atttttcacg ttcgcccggc aggtagcccc acacttcatc
     9481 agcggtgaag gagaggattg gtgccatcca gcgcaccagc gcttctgcga tgtgatacag
     9541 cgcagtctgg cagctacgac gcgccacact gtccgctttg gcggtgtact gacggtcttt
     9601 gatgatgtcg aggtagaagg aacccatctc aacggagcag aagcgcatca gacgctgtac
     9661 cacttcgtgg aaatcgtatg cttcgtacgc cttgaggatg tcttcctgtg ccgctttcgc
     9721 acaacctacg gcccagcgat ccagtaccac catctcttcc ggtttcacca tatcttttgc
     9781 tggatcaaaa ccgttcaggt ttgccagcag gaagcgcgcg gtgttacgga tacgacgata
     9841 gctatcggca gcacgtttca ggatctcgtc agaaacggcc atttcaccag tgtagtcggt
     9901 tgatgccacc cacagacgca gaatatccgc gcccagtttg ttcatcacat cctgcggcga
     9961 aacggtattg ccgatggatt tagacatctt gcgaccctga ccatccacgg taaagccgtg
    10021 ggtcagtacc tgacgatacg gcgctttgcc tttcatcgcg gtggagatca ttagggaaga
    10081 catgaaccaa ccacggtgct ggtcagaacc ttccagatac atgtccgctg cgtgaccggc
    10141 aaattccgga cgcacgtcaa caacagaaga gtgggtagat ccggagtcaa accatacatc
    10201 caatgtgtcc ggcactttca cgtactgatc agcttcatcg ccgaggatct ctttcgcatc
    10261 aagatcccac cacgcctgga tgccgtcaac ttcaacgcgt tttgccactt cttccattag
    10321 ttcgagagta cgcggatgca gctcttccgt gtctttgtgc acgaacagtg acatcggtac
    10381 gccccaggtg cgctgacggg agatacacca gtcaggacgg ttagcaacca tcgactcgat
    10441 acgcgcctgg ccccagtccg ggatccactg cacgcctttg atctctttca gtgactgcgc
    10501 acgcagacct ttctgatcca tgctgacgaa ccactgcggc gtcgcgcgga agatgatcgg
    10561 cgttttgtga cgccagcagc acggatagct gtgctgcatt ttctcaacgt gcagcagcgc
    10621 gcctttttcc tgcagcagcg caacgacgat gtcgttcgct ttgaagacgt tcacgccatc
    10681 cagcgtcgga taagtgcccg gcagataagt gccgtccggg ccaaccgggt tagcggtttc
    10741 caggccgtat ttctgaccga tcacatagtc gtccgggccg tggccaggcg cggtgtgaac
    10801 ggcaccggta ccggcatcta gggtaacgtg atcgccgagg attgccggaa cgtcgaagcc
    10861 cataaacgga tgggtaaagc gcagcaactc aagctccgca ccttttaccg tgccgagaat
    10921 ggtgtaatcg gtcacgccga tacgctgcat tacgctttca accagatctt tcgccagaat
    10981 cacggcctga ccgtcgatct gcaccagcgc atagtcgaaa tcaggtgcaa tagagattgc
    11041 gcggttcgca ggcagagtcc acggcgtagt ggtccagatc accagcgaga ttgggccgtt
    11101 aacgttgctt acggcaaatt ttgctttcag tgcatcctga tcgaccgcct gaaaagcaac
    11161 gtcgatggac ggagaagttt tgtcgtaata ctcaacttcc gcttccgcca gcgcagaacg
    11221 acagtcaacg caccagtgaa ctggcttcgc gcctttgtgc aggtgaccgt tgccgatgat
    11281 tttgcccagc gcgcggatga tgttggcttc agttttgaag tccatggtca ggtacgggtg
    11341 cgaccagtcg cccagcacgc ccagacggat aaagtctttg cgttgaccgt caacctgggt
    11401 cgccgcgtat tcgcggcact tggcgcggaa ctcggcggcg gtgaatttct cacccggctt
    11461 accgtattct tgctctactt tcagctcgat cggcagaccg tggcagtccc agccaggcac
    11521 atacggcgag tcataaccgg aaagcccttt ggacttcacg ataatgtctt tcagaatctt
    11581 gttaaccgag tgaccaatat gaatgctgcc attcgcataa ggagggccat catgcagaat
    11641 gaaggttttt ttgccttttt tagccgcacg gatgatgccg tacagatcat catcagtcca
    11701 acgcgccagc attcccggtt cgcgcttggc gagatcgcca cgcatcggga accctgtttc
    11761 cggcaaattc agggttgatt tatagtcact catcagattc tcggttccgt gtttcggttt
    11821 gattacataa caggcttaag ccggttttgt tagcccaaaa aattcgcggg cggttaattc
    11881 atcacgcgca atctgcgctt tcagttcgtc cagcgacgca aatcgctgct cattgcgtat
    11941 ttttttacgc agcactactt gtatatggcg accgtaaagg tccattgcaa catctaacaa
    12001 atgcacttcc agctgctggc gaataccggc aaccgttggg cgtgttccga tgtttgccac
    12061 gccgggtaac ggcttttcac cgaggcccag cacttctacc gcataaaccc ctttcaccgg
    12121 ggaaacctga cggcgtagcg gtacattcgc cgtcgggaaa cctatagtgc gccctaattc
    12181 atcaccgtgg actacacgcc cggagatggc aaacgggtgc cccagtaaac tttctgccag
    12241 agccagattg tcatccgcca gcgcctgacg cacagcggtg ctgctaatac gcacgccacc
    12301 ttcgcaaaaa gtttgcgtac tagtgatatc gaagccgtat tccatgccag ctttctgtaa
    12361 taacaagaaa tcgccttcac gaccagcgcc aaagcggaaa tcatccccta cggcaagaaa
    12421 ttttacgcgc aaatgcttca ccagaagatc gctgatgaaa ttttgcgcgg ttaacgccgc
    12481 gaaacgcctg tcgaaacgca cgcacagcac gtaatcaacg ccacactctg caaggtaacg
    12541 cagtttttcc cgcagccggg tcagtcttgc cggggcttta tcggtagcaa acagttccag
    12601 tggttgaggt tcaaaaagca tcaccatcac cggtaagttg cgcttgcgcc cttcttcctg
    12661 caagccctgt aacagcgcgc gatgaccgcg atgcacgccg tcgaaattac caatagtcag
    12721 cacacaccct tcttgcgggg cctggctgag attatgtatg ccgcgtatca gcttcatgtc
    12781 tggctcaaaa cagtgaaaat cgtccgagta taccttgtac agcggtcaag gttaaccggc
    12841 gattgagtac cgaaatcaga aagaagtggc ttttcattgc catggcgcaa atcacgggaa
    12901 gaaagtgacc gcctgctgca atttttatcg cggaaaagct gtattcacac cccgcaagct
    12961 ggtagaatcc tgcgccatca ctacgtaacg agtgccggca cattaacggc gcttatttgc
    13021 acaaatccat tgacaaaaga aggctaaaag ggcatattcc tcggcctttg aattgtccat
    13081 atagaacaca tttgggagtt ggaccttggc taatatcaaa tcagctaaga agcgcgccat
    13141 tcagtctgaa aaggctcgta agcacaacgc aagccgtcgc tctatgatgc gtactttcat
    13201 caagaaagta tacgcagcta tcgaagctgg cgacaaagct gctgcacaga aagcatttaa
    13261 cgaaatgcaa ccgatcgtgg accgtcaggc tgctaaaggt ctgatccaca aaaacaaagc
    13321 tgcacgtcat aaggctaacc tgactgcaca gatcaacaaa ctggcttaat cgccaatttg
    13381 ctgaagcttt gtgaaaaagc ccgcgcaagc gggttttttt atgcctgctg cttttatgat
    13441 gcgtgagaga acacactacc ggttgccaga tagtcaaaag atgctggctt ttgcagacat
    13501 gccgttacac tt
//
LOCUS       DAKISA010000112        13498 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.112, whole genome shotgun sequence.
ACCESSION   DAKISA010000112 DAKISA010000000
VERSION     DAKISA010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13498
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.112"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..640
                     /gene="idnO"
                     /locus_tag="OVW45_003474"
     CDS             <1..640
                     /gene="idnO"
                     /locus_tag="OVW45_003474"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HCV5527836.1"
                     /translation="AERAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGP
                     IDVLVNNAGIQRRHPFTEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINIC
                     SMQSELGRDTITPYAASKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVED
                     EAFTAWLCKRTPAARWGDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            705..2024
                     /gene="idnT"
                     /locus_tag="OVW45_003475"
     CDS             705..2024
                     /gene="idnT"
                     /locus_tag="OVW45_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HCV5527837.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            2091..3089
                     /gene="idnR"
                     /locus_tag="OVW45_003476"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             2091..3089
                     /gene="idnR"
                     /locus_tag="OVW45_003476"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HCV5527838.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSVHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLDKRVRRKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            3167..4669
                     /locus_tag="OVW45_003477"
     CDS             3167..4669
                     /locus_tag="OVW45_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCV5527839.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAEHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4788..5870)
                     /gene="lptG"
                     /locus_tag="OVW45_003478"
     CDS             complement(4788..5870)
                     /gene="lptG"
                     /locus_tag="OVW45_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCV5527840.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVASQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5870..6970)
                     /gene="lptF"
                     /locus_tag="OVW45_003479"
     CDS             complement(5870..6970)
                     /gene="lptF"
                     /locus_tag="OVW45_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCV5527841.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVVLDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            7237..8748
                     /gene="pepA"
                     /locus_tag="OVW45_003480"
     CDS             7237..8748
                     /gene="pepA"
                     /locus_tag="OVW45_003480"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCV5527842.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8882..9325
                     /gene="holC"
                     /locus_tag="OVW45_003481"
     CDS             8882..9325
                     /gene="holC"
                     /locus_tag="OVW45_003481"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCV5527843.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            9325..12180
                     /gene="valS"
                     /locus_tag="OVW45_003482"
     CDS             9325..12180
                     /gene="valS"
                     /locus_tag="OVW45_003482"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCV5527844.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(12236..13432)
                     /locus_tag="OVW45_003483"
     CDS             complement(12236..13432)
                     /locus_tag="OVW45_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HCV5527845.1"
                     /translation="MAQVINDMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLIGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMALGMGTV
                     FFISTKMLHANSSSSVIISVVLMTIVCIVFIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIM
                     PSLPFL"
BASE COUNT         3462 a         3413 c         3496 g         3127 t
ORIGIN      
        1 tgccgaacgc gcagaacttg ctgtagaaaa actccaccag gagggtattc aggccgttgc
       61 cgcacctttt aatgttactc ataaacatga aattgatgcc gccgttgaac atatcgaaaa
      121 ggacatcggc cccattgatg tgctggtgaa taacgccggt atccagcgcc gtcatccttt
      181 tactgagttc cctgaacaag agtggaatga tgtgatcgca gtaaaccaga ccgccgtgtt
      241 cctggtatcg caagcggtaa ctcgtcacat ggttgaacgc aaggcaggta aagttattaa
      301 tatttgctcg atgcaaagcg aactgggacg tgacaccatc accccttatg ccgcatcgaa
      361 aggggcggta aaaatgctca cccgcggcat gtgcgtcgag ctggcgcgcc acaatattca
      421 ggtcaacggt attgcgccgg gctatttcaa aacagaaatg actaaagcac tggttgagga
      481 cgaagccttc accgcctggt tgtgcaaacg gacccccgcc gcacgctggg gagatccgca
      541 ggaactgatt ggtgctgcgg tgttcctttc ttcaaaagcc tctgatttcg taaacggcca
      601 cctgttgttt gttgatggcg gcatgttagt ggctgtttaa cagccaccac cctacgtgca
      661 gcgcgaaagc gctgcttttc tggcactaat aagagataaa gactatgcca ttaatcatta
      721 ttgcggcagg cgtcgcgctg cttcttatcc tgatgatcgg ctttaaagtt aacggcttta
      781 ttgccctcgt tctggtagct gccgtcgtcg gatttgccga agggatggat gcacaggccg
      841 tcctgcactc tatacaaaat ggtatcggca gcacgctcgg cgggctggca atgatcctcg
      901 gtttcggggc catgttaggc aagctgattt ctgatacggg cgcggcacaa cgtatcgcca
      961 ctacgctgat tgctactttt ggtaaaaaac gcgtgcaatg ggcgctagtg atcaccggtc
     1021 tggttgtggg cctcgccatg ttttttgaag tgggttttgt cctgctgttg ccgttggtat
     1081 ttaccatcgt ggcatcatct ggattacccc tgttgtatgt tggcgtaccg atggtagcag
     1141 cgctctctgt aacccactgt tttctgccgc cacatccagg gcctactgcc atcgcgacta
     1201 tctttgaggc taatctcgga acgactttac tgtatggatt tatcattacc attccgacag
     1261 ttattgtcgc aggaccgctg ttttctaaac tgctaactcg ctttgagaaa gcaccaccgg
     1321 aaggcttatt taatcctcat ctgtttagcg aagaggagat gccctccttc tggaacagta
     1381 tttttgctgc agtgatcccg gtcatcctga tggctatcgc cgccgtttgt gaaattacgt
     1441 taccgaaaac taacaccgtg cgcctcttct ttgaatttgt cggtaaccct gccgttgcgc
     1501 tgtttattgc cattgttatt gcgattttca cactgggccg acgtaatgga cgcaccatcg
     1561 agcaaatcat ggatatcatt ggggattcta taggcgctat cgcgatgatt gtgtttatta
     1621 tcgctggcgg cggcgcgttt aagcaggtat tagtagatag tggtgtcggg cagtatattt
     1681 cacacttaat gaccggaact acgctttcgc cgttattgat gtgctggact gtcgcggcgc
     1741 tgttgcgtat cgctctgggc tctgccaccg tcgcggccat taccaccgcg ggtgtggtgt
     1801 tgccgattat caacgttacc catgccgatc ccgctttaat ggtactggca actggtgcgg
     1861 gcagcgtgat cgcgtcacac gtaaacgacc ctggcttctg gctatttaaa gggtatttta
     1921 atctgacggt tggtgaaacg ttgcgtacct ggacagtgat ggaaaccctt atttctatta
     1981 tgggtttgct gggcgtgtta gccattaacg ccgtattgca ctgaaagaat aaaatattgt
     2041 gatgtagttt cgggggaaga ttgaagattc ccccgacgtt gggagaagtt atgcgcaatc
     2101 acagaatttc tttacaggat atcgctacgc tggctggcgt aacaaaaatg accgtgagtc
     2161 gttatatccg ctcgccgaaa aaggtggcaa aggaaacagg cgagcgcatc gccaaaatta
     2221 tggaagagat caattatatc cctaatcgcg ctcccggcat gttgttgaat gcacaaagtt
     2281 ataccctcgg tatcttgatt ccgtcatttc aaaatcaact cttcgctgac attctcgcgg
     2341 gaatagaatc cgtcacctct gtgcataatt atcagaccct tattgctaat tacaattacg
     2401 atcgcgattc cgaagaagag tcggtgatta acctgctctc ttataacatt gacgggatta
     2461 ttctttcgga aaaatatcac accatcagga cggtgaaatt tctgcgttca gccaccattc
     2521 cggtcgtgga attgatggat gtacagggag aacggctgga tatggaggtc ggttttgata
     2581 atcggcaggc tgcttttgac atggtgtgta ccatgctgga taagcgagtc aggcgcaaaa
     2641 ttctgtatct cggttccaaa gatgacaccc gcgatgaaca gcgttatcag gggtattgcg
     2701 atgcgatgat gctgcataat ctttccccat tacgcatgaa tccacgcgcc atctcatcca
     2761 ttcatttagg aatgcagtta atgcgtgatg cccttagcgc aaacccggat ttagacggtg
     2821 tattttgcac caatgacgat attgcgatgg gtgcgcttct gctgtgccgc gagcgaaacc
     2881 tcgccgtgcc agaacaaatt tccattgcgg gttttcatgg gctggagatt ggcaggcaaa
     2941 tgatcccaag cctcgccagc gtaattactc cccgttttga tattggtcgg atggccgccc
     3001 aaatgttgct gagcaagatt aagaataacg atcacaacca caacactgtt gacctcgggt
     3061 atcagattta tcacggtaac acgctttagg ttattttctc ttcattacac atattctctt
     3121 tgccggacgt tgcctatact ccaggcagga aactggagga aatctcatga gcgaacccct
     3181 gttaattgcc cgcacgccgg acacagaact gtttttactg ccgggaatgg ctaaccgtca
     3241 cgggctgatt actggcgcaa cggggacggg taaaactgtc acgttgcaaa aactggcaga
     3301 gtcattgtcg gaaatcggtg ttccggtgtt tatggccgat gtgaaaggcg atctgaccgg
     3361 catcgcgcag gcaggaacgg cgtcggaaaa actgctcgca agactgaaaa atatcggcgt
     3421 caacgactgg caaccgcatg ccaatccggt ggtggtgtgg gatatctttg gcgagaaagg
     3481 ccatccggtg cgggcgacgg tttcggatct ggggccgctg ttgctggcac gactgttgaa
     3541 tctcaacgat gtgcaatctg gcgtgctgaa tattatcttc cgcattgcgg acgatcaggg
     3601 attgttgctg ctcgacttta aagatttgcg ggcgattacc cagtacatcg gcgataacgc
     3661 caaatccttc cagaatcagt acggaaatat cagtagcgca tcggttggtg ccatccagcg
     3721 cggattactg tcgctggaac agcaaggcgc agaacacttc tttggtgagc cgatgctgga
     3781 tatcaaagac tggatgcgca ccgatgccaa cggtaaaggc gttatcaata tcctcagcgc
     3841 cgagaaactt tatcagatgc cgaaactgta cgccgccagc ctgctgtgga tgctttcaga
     3901 gttatatgaa caattgcctg aagcgggcga tctggagaaa ccaaaactgg tgtttttctt
     3961 cgacgaagca catctgctgt ttaacgatgc gccacaggta ctgctggata agattgagca
     4021 ggtgataagg cttattcgct caaaaggcgt gggcgtctgg ttcgtttcgc aaaacccgtc
     4081 agatatcccg gataacgtgc tagggcagct cggtaatcgc gttcaacacg ctttgcgtgc
     4141 ttttacgcct aaagatcaga aagcagtgaa ggcagcggcg caaaccatgc gggccaatcc
     4201 ggcgtttgat accgaaaagg caatccagga actggggacc ggcgaggcgt taatctcgtt
     4261 tctcgatgcg aaaggaagtc cttcagtggt ggaacgggcg atggtgatcg caccttgttc
     4321 gcgaatgggg ccggtgacgg aagatgagcg taatggcctg attaatcact ccccggtgta
     4381 tggcaaatat gaagatgagg tggaccgcga gtccgcctat gagatgctgc aaaaaggctt
     4441 tcaggccagt accgagcagc aaaataatcc ccccgtgaaa gggaaagagg tggcggtgga
     4501 tgacggtatt cttggtggat tgaaggatat tttgtttggc actaccggac cacgcggcgg
     4561 gaagaaagat ggtgtggtgc aaacaatggc gaaaagcgcc gctcgccagg tgacgaatca
     4621 gattgtacgc gggatgttgg ggagtttgct gggggggaga aaaaggtaag tcagacgtga
     4681 tctgtgcccc tcaccctaac cctctcccca aaggggcgag gggaccgttc gaactcgtat
     4741 ttgactttgt caacaataat aaagggagct ttcgctccct tgttagatta cgattttctc
     4801 attaacagcc acaggctgat taagaagaag ctggcgcttg gcaacagtgc gccgatgatc
     4861 ggcgggatgc cataaaccaa cgtcagcggg ccgaagatct ggtccagtac gtagaagaca
     4921 aaaccgaagc tgataccggt gaccacacgc acgcccatcg gtacgctacg cagtgggcca
     4981 aagatgaacg acagcgccat caacatcatc accgccacgg atagcggctg gaagattttg
     5041 ctccacatgt tgagctgata acgtccggca tcctgaccgc tcgacttcag atacttcaca
     5101 tagttgtgca aaccactgat ggagagtgca tccgggtcca gcgccaccac gcccagtttg
     5161 tctggcgtga ggttggtttt ccaggtgccg ctcaccgtct gcgaaccggt gatctgtttc
     5221 ggattggtca gatcagattc atcaacctgc gacagacgcc agactttatg ttccgggtca
     5281 aacttcgcgg tagcggcata acgtacggat tgcagacgac gattctcgtt aaaggcataa
     5341 atactgatgc cacctaactc ttcgtcacct ttaacccgct caatgtagac gaagttgttg
     5401 ccatctttcg cccataagcc ttgctgagtg gagagcaacg agccgccgta catcgcctgc
     5461 gcacggtagt tacgcgccat ctgctcgccc tgcgacgcga cccattcacc aatcgccatc
     5521 gtcagcaaga ccagcggaat ggcggttttc atcaccgaca gcgccacctg catacgggta
     5581 aaaccagaag cctgcatcac caccagttcg ctgcgctgcg ccagcatccc aagaccaagc
     5641 aacgccccaa gcagagccgc catcgggaag aagatttgca catctttcgg cacgctcagc
     5701 aaggtataca ttcctgcgcc taacgcgtcg taactcccct gcccggcttt tttcagctga
     5761 tcgacaaact tgataatgcc cgacagcgac accagcatga acagcgtcat catgatggtg
     5821 gtgaaaatag ttttaccgat atagcggtca agtacgccaa aaggttgcat cacaccgctc
     5881 ctttacgcga aaaactggcg cgcaggcggc ggaccggcac ggtgtcccaa aggttgagaa
     5941 caatcgccaa agccagataa atcaggttaa cggtccacat ccacagcgtc gggtccagct
     6001 tacctttacc gccgttcgat ttcagggagg tctggatcag gaagaaaagt agatacagca
     6061 gcatggctgg cagcatcgac agtacgcgtc cctgacgcgg gttaaccacg ctcaacggta
     6121 caaccataag tgccatcata aacacggtga ataccaacgt gatacgccag ttcagttctg
     6181 cgcgggcacg atcggtgtca gtgttccaca atgtgcgcat gtccatctgg tcggtatcgt
     6241 tcgggtcgag caccaccgcc tggtgaccaa tgatcgcctg ataatcctga aagtccgtaa
     6301 tgcggaaatc gcgtaacagt gcagtgcctt cgaagcgcgt tccctggttg agagtgacga
     6361 cctgggagcc gtcgcgcagc tgggttaaat gtccggaatc ggccaccacc acagaaggac
     6421 gtgcattacc ttttggtcga atttgtgcga ggaacacatc tttgaaatcg ctgccgtcaa
     6481 cgctttcgat gaacagcacc gagctgccat tagtcgcttg ctggaattgc ccttgcgcca
     6541 gcgccgccat gccagggttc gctttcgctt ctgctaacac ttcatcctga tgacgcgatg
     6601 accatggtcc cgcccacatc acgttaaccg ccgcgacgat tgccgtgaac accgcaagga
     6661 tcattgccgc tttcaccagc accgctttgc tcagaccgca ggcatgcatt accgtaattt
     6721 cactttcggt atacagtttg cccagcgtca tcagcagccc gaggaacagg cttaatggca
     6781 ggataagctg cgccatttcc ggcacgccca acccgagaag ggagagcacc agattcgccg
     6841 gaatatcgcc gtcaaccgct gcgccgagga tcctcactaa cttttgacag aagaagatca
     6901 aaagcaagat gaagagtatc gccagctggc ttttgagcgt ctcccgcacc agatatctta
     6961 tgattatcac tttaaatacg cccgtaaaaa ctcgtctttt gcaggatttt agcttgtttc
     7021 atggcttaaa cgtcatttat tctcttgagt cgtcgaaatc gtcgctaaga taattatact
     7081 caacggattc acctctcaga ttttgttctg acgtgccaat gccgtaataa cgttaagatt
     7141 aacacgaagt catcgcaaca gcggacatga gttacgaaag cttgcaattc tatctgtagc
     7201 caccgccgtt gtctttaaga ttcaggagcg tagtgcatgg agtttagtgt aaaaagcggt
     7261 agcccggaga aacagcggag tgcctgcatc gtcgtgggcg tcttcgaacc acgtcgcctt
     7321 tctccgattg cagaacagct cgataaaatc agcgatgggt acatcagcgc cctgctacgt
     7381 cggggcgaac tggaaggaaa accggggcag acactgttgc tgcaccatgt tccgaatgta
     7441 ctttccgagc gaattctcct tattggttgc ggcaaagaac gtgagctgga tgaacgtcag
     7501 tacaagcagg ttattcagaa aaccattaat acgctgaatg atactggctc aatggaagcg
     7561 gtctgctttc tgactgaact gcacgttaaa ggccgtaaca actactggaa agtgcgtcag
     7621 gctgtcgaga ctgcaaaaga gacgctctac agtttcgatc agctgaaaac gaacaagagc
     7681 gaaccgcgtc gtccgctgcg taaaatggtg ttcaacgtgc cgacccgccg tgaactgacc
     7741 agcggtgagc gcgcgatcca gcacggtctg gcgattgccg ccgggattaa agcagcaaaa
     7801 gatctcggca atatgccgcc gaatatctgt aacgccgctt acctcgcttc acaagcgcgc
     7861 cagctggctg acagctacag caagaatgtt atcacccgcg ttatcggcga acagcagatg
     7921 aaagagctgg ggatgcactc ttatctggcg gtcggtcagg gttcgcaaaa cgaatcgctg
     7981 atgtcggtga ttgagtacaa aggcaacgcg tcggaagatg cacgcccaat cgtgctggtg
     8041 ggtaaaggtt taaccttcga ctccggcggt atctccatca agccttcaga aggcatggat
     8101 gagatgaagt acgatatgtg cggtgcggca gcggtttacg gcgtgatgcg gatggtcgca
     8161 gagctacaac tgcctattaa cgttattggc gtgctggcag gctgtgaaaa catgcctggc
     8221 gggcgtgcct atcgtccggg cgatgtgtta accaccatgt ccggtcaaac cgttgaagtg
     8281 ctgaacaccg atgccgaagg ccgcctggta ctgtgcgacg tgttaactta cgttgaacgt
     8341 tttgagccgg aagcggtgat tgatgtggcg acgctgaccg gtgcctgcgt gatcgcgctg
     8401 ggtcatcata ttactggtct gatggcgaac cataatccgc tggcccatga actgattgcc
     8461 gcgtctgaac aatccggtga ccgcgcatgg cgcttaccgc tgggtgacga gtatcaggaa
     8521 caactggagt ccaattttgc cgatatggcg aacattggcg gtcgtcctgg tggggcgatt
     8581 accgcaggtt gcttcctgtc acgctttacc cgtaagtaca actgggcgca cctggatatt
     8641 gcaggaaccg cctggcgttc tggtaaagca aaaggcgcaa ccggtcgtcc ggtagcgttg
     8701 ctggcacagt tcctgttaaa ccgcgctggg tttaacggcg aagagtaagt ttgcgtttta
     8761 ccctcaccct aaccctctcc ctaagggaga gggaaccgat cgagcgcaat agaaattcat
     8821 tgctcatctt tcagggtgag gaatgcagca atatttaaat ccaccacaag aagccccata
     8881 tatgaaaaac gcaacgttct accttctgga caatgacacc accgtcgatg gcttaagcgc
     8941 cgttgagcaa ctggtgtgtg aaattgccgc agaacgttgg cgcagcggta agcgcgtgct
     9001 catcgcctgt gaagatgaaa agcaggctta ccggctggat gaagccctgt gggcgcgtcc
     9061 ggcagaaagc tttgtcccgc ataatttagc gggagaagga ccgcgcggcg gtgcgccggt
     9121 ggagatcgcc tggccgcaaa aacgcagcag cagcccgcgc gatatattga ttagtctgcg
     9181 aacaagcttt gcagattttg ccactgcttt cacagaagtg gtagacttcg ttccttatga
     9241 agattctctg aaacaactgg cgcgcgaacg ctataaagcc taccgcgtgg ctggtttcaa
     9301 cctgaatacg gcaacctgga aataatggaa aagacatata acccacaaga tatcgaacag
     9361 ccgctttacg agcactggga aaagcagggc tactttaagc ctaatggcga tgaaagccag
     9421 gaaagtttct gcatcatgat cccgccgccg aacgtcaccg gcagtttgca tatgggtcac
     9481 gccttccagc aaaccatcat ggataccatg atccgctatc agcgcatgca gggcaaaaac
     9541 accctatggc aggtcggtac tgaccacgcc gggatcgcta cccagatggt cgttgagcgc
     9601 aagattgccg cagaagaagg taaaacccgt cacgactacg gccgcgaagc tttcatcgac
     9661 aaaatctggg aatggaaagc ggagtccggc ggcaccatta cccgtcagat gcgccgtctc
     9721 ggcaactctg tcgactggga gcgtgaacgc ttcacgatgg acgaaggcct gtccaatgcg
     9781 gtgaaagaag ttttcgttcg tctgtataaa gaagacctga tttaccgtgg caaacgcctg
     9841 gtaaactggg atccgaaact gcgcaccgct atctctgacc tggaagtgga aaaccgcgaa
     9901 tcgaaaggtt cgatgtggca catccgctat ccgctggctg acggcgcgaa aaccgcagac
     9961 ggtaaagatt atctggtggt tgcgactacc cgtccggaaa ccctgttggg cgataccggc
    10021 gtggccgtta acccggaaga tccgcgttac aaagacctga ttggcaaata tgtcattcta
    10081 ccgctggtta accgtcgtat tccgatcgtt ggcgacgaac acgccgacat ggaaaaaggc
    10141 accggctgcg tgaaaatcac tccggcgcac gactttaacg actatgaagt gggtaaacgt
    10201 cacgcgctgc cgatgatcaa catcctgacc tttgacggcg atatccgtga aagcgcccag
    10261 gtgttcgata ccaaaggtaa cgaatctgac gtttattcca gcgaaatccc ggcagagttc
    10321 cagaaactcg agcgttttgc tgcacgtaaa gcagtcgttg ctgcggttga cgcactcggc
    10381 ctgctggaag aaattaaacc gcacgacctg accgttcctt acggcgaccg tggcggcgta
    10441 gttatcgaac caatgctgac cgaccagtgg tacgtgcgtg ccgatgtact ggcgaaaccg
    10501 gcggttgaag cggttgagaa cggcgacatt cagttcgtac cgaagcagta cgaaaacatg
    10561 tacttctcct ggatgcgcga tattcaggac tggtgtatct ctcgtcagct gtggtggggt
    10621 caccgtatcc ctgcatggta cgacgaagca ggtaacgttt atgttggccg caacgaagaa
    10681 gaagtgcgca aggaaaataa cctcggtgcc gacgttgccc tgcgtcagga cgaagacgtt
    10741 ctcgacacct ggttctcttc tgcgctgtgg accttctcta ccctaggctg gccggaaaac
    10801 actgacgcac tgcgccagtt ccacccaacc agcgtgatgg tatccggctt cgacatcatt
    10861 ttcttctgga ttgcccgcat gatcatgatg accatgcact tcatcaaaga tgaaaatggc
    10921 aaaccgcagg tgccgttcca caccgtttac atgaccggcc tgattcgtga cgacgaaggc
    10981 cagaagatgt ccaaatccaa gggtaacgtt attgacccgc tggatatggt tgacggtatt
    11041 tcgctgccgg aactgctgga gaaacgtacc ggcaatatga tgcagccgca gctggcggac
    11101 aaaatccgta agcgcaccga gaagcagttc ccgaacggta ttgagccgca cggcaccgat
    11161 gccctgcgct tcaccctggc ggcgctggcg tctaccggtc gtgacatcaa ctgggatatg
    11221 aagcgcctgg aaggttaccg taacttctgt aacaagctgt ggaacgccag ccgctttgtg
    11281 ctgatgaaca cggaaggtca ggattgcggc ttcaacggtg gtgaaatgac gctgtcgctg
    11341 gcggaccgct ggattctggc agagttcaac cagaccatca aagcgtaccg cgaagcgctg
    11401 gacagcttcc gcttcgatat cgctgcaggc attctgtatg agttcacctg gaaccagttc
    11461 tgtgactggt atctggagct gactaagccg gtaatgaacg gtggcaccga agccgaactg
    11521 cgcggtactc gccatacgct ggtgaccgta ctggaaggtc tgctgcgcct cgcgcatccg
    11581 atcattccgt tcatcaccga aaccatctgg cagcgtgtga aagtactttg cggtatcact
    11641 gccgacacca tcatgctgca gccgttcccg cagtacgatg catctcaggt tgatgaagcc
    11701 gcactggccg acaccgaatg gctgaaacag gcgatcgttg cggtacgtaa catccgtgca
    11761 gaaatgaata tcgcgccggg caaaccgctg gagctgctgc tgcgtggttg cagcgcggat
    11821 gcagaacgtc gcgtaaatga aaaccgtggc ttcctgcaaa ccctggcgcg tctggaaagc
    11881 atcaccgtgc tgcctgccga tgacaaaggt ccggtttccg ttacgaagat catcgacggc
    11941 gcagagctgc tgatcccgat ggctggcctc atcaacaaag aagatgagct ggcgcgtctg
    12001 gcgaaagaag tggcgaagat cgaaggcgaa atcagccgta tcgagaacaa gctggcgaat
    12061 gaaggctttg tcgcccgcgc accggaagcg gtcatcgcga aagagcgtga gaagctggaa
    12121 ggctatgcgg aagcgaaagc gaaactgatt gaacagcagg ctgttatcgc cgcgctgtaa
    12181 tcaaaacaca gtgatgaaaa cgaaggccgg agcatgctcc ggcctttttt atctcttaca
    12241 gaaatggtaa agaaggcata attccccgtg aaatactggc taagaatcct ttatctggtt
    12301 gttcttcttt attgattaaa ggaaggtcat ccaaatcgcc cagcaaatac gtattttttg
    12361 cctgccagtc aatcatccat attttcagca gtggataagc cagaccgccc gtaatcccgg
    12421 atatcaccac caacgcacac atgcgataaa gcataccgtg gtatgttaaa gttgagcgaa
    12481 aacgaatacg cccatcattc agtgacaggt tgctcataaa atgatttcgc aacgacaccg
    12541 ttaaataact ggtgctaaca gcaatcccaa aataatagat taactgcgcg attatcattt
    12601 tacgttgcat ttccataaat tgctgtaaat tctcaatatc atcatttgca tacccagaac
    12661 tatcatactc atttaatatt tgatcgaaga taatataacc agcaacagcg agaaaaggta
    12721 ataaagccaa aaatgcgaga atggcatatt ttatacaata tgtagtatct aatttcacct
    12781 tgaaacgatg tataccgaaa ctggtattac tccagagaaa actcattacc agactatata
    12841 aagtgccatt aaaaatacca atgaaaacaa tacaaactat tgtcatcaga accacagata
    12901 taataacgct acttgaacta ttggcatgta gcatctttgt cgagataaag aaaacagtcc
    12961 ccatcccaag ggccattaaa atcggcaaga aaaaggtcac ccaccagaac cctttcatag
    13021 agcaattaaa actaaatctt acgccgttga gactggtcat caaggcctga taacgcagtc
    13081 cttttgctgc cataaatata agcaaaacca acagtgacaa agtcaaaaca cagccaataa
    13141 gcggcatatc tgctgacact gtcattaaga ttgcgaaata gaaaaaaaca aaaacaagac
    13201 aactaacaaa aacattccca ccggtaatcc cataagaaaa tcgttgtccg ttaacttcca
    13261 tattagcata gagataacgc ttacatttca ttaatgccca tggtaaatag atacctagcg
    13321 ttataatcgt taacaacaca ttcaccacac aaataagaaa atatctctca cctgtaccat
    13381 gaaaaacaaa cgaatgggac ggaacatcca tatcattaat aacttgagcc ataaaagttc
    13441 cctgaattaa aaaatccatt gatataattt atatatacag acagtaaaat gttctgcc
//
LOCUS       DAKISA010000113        13471 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.113, whole genome shotgun sequence.
ACCESSION   DAKISA010000113 DAKISA010000000
VERSION     DAKISA010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13471
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.113"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            322..3108
                     /gene="polA"
                     /locus_tag="OVW45_003484"
     CDS             322..3108
                     /gene="polA"
                     /locus_tag="OVW45_003484"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCV5527846.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            complement(3490..4122)
                     /locus_tag="OVW45_003485"
     CDS             complement(3490..4122)
                     /locus_tag="OVW45_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCV5527847.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            4704..5213
                     /gene="yihI"
                     /locus_tag="OVW45_003486"
     CDS             4704..5213
                     /gene="yihI"
                     /locus_tag="OVW45_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCV5527848.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVAEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            5402..6775
                     /gene="hemN"
                     /locus_tag="OVW45_003487"
     CDS             5402..6775
                     /gene="hemN"
                     /locus_tag="OVW45_003487"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCV5527849.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(6965..7075)
                     /locus_tag="OVW45_003488"
     CDS             complement(6965..7075)
                     /locus_tag="OVW45_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCV5527850.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(7187..8596)
                     /gene="glnG"
                     /locus_tag="OVW45_003489"
     CDS             complement(7187..8596)
                     /gene="glnG"
                     /locus_tag="OVW45_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCV5527851.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            complement(8608..9657)
                     /gene="glnL"
                     /locus_tag="OVW45_003490"
     CDS             complement(8608..9657)
                     /gene="glnL"
                     /locus_tag="OVW45_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCV5527852.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            complement(9831..11240)
                     /gene="glnA"
                     /locus_tag="OVW45_003491"
     CDS             complement(9831..11240)
                     /gene="glnA"
                     /locus_tag="OVW45_003491"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCV5527853.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            11613..13436
                     /gene="typA"
                     /locus_tag="OVW45_003492"
     CDS             11613..13436
                     /gene="typA"
                     /locus_tag="OVW45_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCV5527854.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
BASE COUNT         3412 a         3417 c         3528 g         3114 t
ORIGIN      
        1 aaacgaccca aataacggat gatccttaag gagaaaaata atccatatct atccacatta
       61 gaaaaaatcc cattatctca attattaggg atggctttat ttttaactgc atgaaaaaca
      121 aagacaaaca tcatgctgta aaaagcatga taataaatta aaagcgatgt aaataattta
      181 tgcacaaagt tatccacatg acgatttgcg agcgatccag aagatctaca aaagattttc
      241 acgaaaagcg gtgaaaaact catgttttca tcctgtctgt ggcatccttt acccataatc
      301 tgataaacag gcacggacat tatggttcag atcccccaaa atccacttat ccttgtagat
      361 ggttcatctt atctttatcg cgcatatcac gcgtttcccc cgctgactaa cagcgcaggc
      421 gagccgaccg gtgcgatgta tggtgtcctc aacatgctgc gcagtctgat catgcaatat
      481 aaaccgacgc atgcagcggt ggtctttgac gccaagggaa aaacctttcg tgatgaactg
      541 tttgaacatt acaaatcaca tcgcccgcca atgccggacg atctgcgagc acaaatcgaa
      601 cccctgcacg cgatggttaa agcgatggga ctgccgctgc tggcggtttc tggcgtagaa
      661 gcggacgacg ttatcggtac tctggcgcgc gaagccgaaa aagccgggcg tccggtgctg
      721 atcagcactg gcgataaaga tatggcgcag ctggtgacgc caaatattac gcttatcaac
      781 accatgacga ataccatcct cggaccggaa gaggtggtga ataagtacgg cgtgccgcca
      841 gaactgatca tcgatttcct ggcgctgatg ggtgactcct ccgataacat tcctggcgta
      901 ccgggcgtcg gtgaaaaaac cgcgcaggca ttgctgcaag gtcttggcgg gctggatacg
      961 ctgtatgccg agccagaaaa aattgctggg ttgagcttcc gtggcgcgaa aacaatggca
     1021 gcgaagctcg agcaaaacaa agaagttgct tatctctcat accagctggc gacgattaaa
     1081 accgacgttg aactggagct gacctgtgaa caactggaag tgcagcaacc ggcagcggaa
     1141 gagttgttgg ggctgttcaa aaagtatgag ttcaaacgct ggactgctga tgtcgaagcg
     1201 ggcaaatggt tacaggccaa aggggcaaaa ccagccgcga agccgcagga aaccagtgtt
     1261 gcagacgaag cgccagaagt gacggcaacg gtgatttctt atgacaacta cgtcaccatc
     1321 cttgatgaag aaacactgaa agcgtggatt gcgaagctgg aaaaagcgcc ggtatttgca
     1381 tttgataccg aaaccgacag ccttgataac atctctgcta acctggtcgg gctttctttt
     1441 gctatcgagc caggcgtagc ggcatatatt ccggttgctc atgattatct tgatgcgccc
     1501 gatcaaatct ctcgcgagcg tgcactcgag ttgctaaaac cgctgctgga agatgaaaag
     1561 gcgctgaagg tcgggcaaaa cctgaaatac gatcgcggta ttctggcgaa ttacggcatt
     1621 gagctgcgtg ggattgcgtt tgataccatg ctggagtcct acattctcaa tagcgttgcc
     1681 gggcgtcacg atatggacag cctcgcggaa cgttggttga agcacaaaac tatcactttt
     1741 gaagagattg cgggtaaagg caaaaatcaa ctgaccttta accagattgc cctcgaagag
     1801 gccggacgtt acgccgccga agatgcggat gtcaccttgc agttgcatct gaaaatgtgg
     1861 ccagatctgc aaaaacacaa agggccgttg aacgtcttcg agaatatcga aatgccgctg
     1921 gtgccggtgc tttcacgcat tgaacgtaac ggtgtgaaga tcgatccgaa agtgctgcac
     1981 aatcattctg aagagctcac ccttcgtctg gctgagctgg aaaagaaagc gcatgaaatt
     2041 gcaggtgagg aatttaacct ttcttccacc aagcagttac aaaccattct gtttgaaaaa
     2101 cagggcatta aaccgctgaa gaaaacgccg ggtggcgcgc cgtcaacgtc ggaagaggta
     2161 ctggaagaac tggcgctgga ctatccgttg ccaaaagtga ttctggagta tcgtggtctg
     2221 gcgaagctga aatcgaccta caccgacaag ctgccgctga tgatcaaccc gaaaaccggg
     2281 cgtgtgcata cctcttatca ccaggcagta actgcaacgg gacgtttatc gtcaaccgat
     2341 cctaacctgc aaaacattcc ggtgcgtaac gaagaaggtc gtcgtatccg ccaggcgttt
     2401 attgcgccag aggattatgt gattgtctca gcggactact cgcagattga actgcgcatt
     2461 atggcgcatc tttcgcgtga caaaggcttg ctgaccgcat tcgcggaagg aaaagatatc
     2521 caccgggcaa cggcggcaga agtgtttggt ttgccactgg aaaccgtcac cagcgagcaa
     2581 cgccgtagcg cgaaagcgat caactttggt ctgatttatg gcatgagtgc tttcggtctg
     2641 gcgcggcaat tgaacattcc acgtaaagaa gcgcagaagt acatggacct ttacttcgaa
     2701 cgctaccctg gcgtgctgga gtatatggaa cgcacccgtg ctcaggcgaa agagcagggc
     2761 tacgttgaaa cgctggacgg acgccgtctg tatctgccgg atatcaaatc cagcaatggt
     2821 gctcgtcgtg cagcggctga acgtgcagcc attaacgcgc caatgcaggg aaccgccgcc
     2881 gacattatca aacgggcgat gattgccgtt gatgcgtggt tacaggctga gcaaccgcgt
     2941 gtacgtatga tcatgcaggt acacgatgaa ctggtatttg aagttcataa agatgatgtt
     3001 gatgccgtcg cgaagcagat tcatcaactg atggaaaact gtacccgtct ggatgtgccg
     3061 ttgctggtgg aagtggggag tggcgaaaac tgggatcagg cgcactaaga ttcgcctgaa
     3121 catgcccttt tttcgtaagt aagcaacata agctgtcacg ttttgtgatg gctattagaa
     3181 attcctatgc aacaactgaa aaaaaattac aaaaagtgct ttctgaactg aacaaaaaag
     3241 agtaaagtta gtcgcgtagg gtacagaggt aagatgttct atctttcaga ccttttactt
     3301 cacgtaatcg gatttggctg aatattttag ccgccccagt cagtaatgac tggggcgttt
     3361 tttattgggc gaaagaaaag atccgtaatg cctgatgcgc tatgtttatc aggccaacgg
     3421 tagaattgta atctattgaa tttacgggcc ggatacgcca catccggcac aagcattaag
     3481 gcaagaaaat tattcgccgt cctgcgtttc ttctacaggc tgcatctcgc taaaccaggt
     3541 atccagtttc tgccgcagct tgtccacgcc ttgtttcttc aacgaagaaa acgtttcaac
     3601 ctgcacatca ccgttaaacg ccagtacagc ttcacgcacc atattcaatt gcgctttacg
     3661 tgcgccgctt gccagtttgt ccgctttggt cagcagcacc agaacggcga tattgctgtc
     3721 taccgcccac tcaatcatct gctgatccaa atctttcagc ggatggcgaa tatccattag
     3781 caccaccaga ccttgcaggc tctgacgttt ttcgaggtat tcgccgagcg cacgctgcca
     3841 tttgcgcttc atctcttccg ggacttccgc ataaccgtac ccaggcaagt caaccagacg
     3901 cttgccgtca gccacttcaa acaggttgat aagctgggtg cgccctgggg tttttgaggt
     3961 acgagccagg cttttctggt tagtcagcgt gttcagcgcg ctggatttac ctgcgttgga
     4021 acggcctgca aaagccactt caattccggt atcggaaggt aggtggcgaa tatcaggcgc
     4081 actcatcaca aaatgcgtct gttgataatt caaattagtc aaagcggtcg tctccgtcag
     4141 tcaaagcttt gtggcgatta tacctgaacc ataataaaag gcggattttt cggcgtgagc
     4201 gttgtaagta aaagccatac gctttgtgag acattgccga tagtctttat gcgaaatagc
     4261 agagaaaatt ctgcgatgca tgtcaaataa gctatataaa tcagtgaatt gcttttatat
     4321 aaaactctga aaatgtgcaa atactgatgg cggttgattg tttgtttaaa gcaaaggcgt
     4381 aaagtagcac ccatagagcg aggacgctaa caggaacaat gactcaggat gagggtcagg
     4441 agcgccagga ggcgaagaca gaggattgtc aggaagacaa acgtccggag acgtaattaa
     4501 acggaaatgg aatcaacacg gattgttccg gctaaaggaa aaacagggtg tgttggcggc
     4561 ctgcaaggat tgtaagaccc gttaagggtt atgagtcagg aaaaaaggcg acagagtaat
     4621 ctgtcgcctt ttttctttgc ttgctttctg ttagattccg ccgcaaatct atactgaata
     4681 aaacggctaa aagacgaacc attatgaaac catcatcttc aaactcacgc agcaaaggtc
     4741 atgcaaaagc gcgtcgaaaa acacgcgagg agctggatca ggaagctcgt gatcgcaagc
     4801 gtcagaaaaa acgtcgtggt catgcgccgg gcagccgcgc agcgggcgga aacaccacgt
     4861 caggcagcaa aggccagaac gcaccaaaag atccacgtat tggcagtaaa acccctattc
     4921 cattgggcgt ggctgaaaaa gtcaccaaac agcacaaacc gaagagtgag aaacctatgc
     4981 tttcaccgca ggcggagttg gaattactgg aaacggatga gcgtctggat gcgctgctgg
     5041 aacgtctgga agcaggcgaa accctgagcg ccgaagagca atcctgggtg gatgctaaac
     5101 tggatcgtat tgatgagttg atgcagaaac tcggcctctc ttatgacgat gacgaagaag
     5161 aggaagaaga cgagaagcaa gaagacatga tgcgtctgct gcggggtaac taacggattg
     5221 ccaccgtggg ccttcccgtt ctgctgataa cccttccggt tatatgttat ctggtgtggt
     5281 tattcgttaa actacagcgg ttgtcgcggc gacaaaagtg gctgcgcaac cggctgatga
     5341 cccgaaacgg gcatccgccg gtacgccgta gccgccagag acgccatcgg aaggagtgag
     5401 catgtctgta cagcaaatcg actgggatct ggccctgatc cagaaatata actattccgg
     5461 gccacgatac acctcgtacc cgaccgcgct ggagttttca gaagacttcg gcgaacaggc
     5521 gtttttacaa gccgtggcgc gctatcctga gcgtccatta tctctctacg tacatatccc
     5581 gttctgccat aagctttgtt acttctgcgg ttgcaataag attgttactc gccagcagca
     5641 caaggccgat cagtatctgg acgcgctgga gcaagaaatc gtccatcgtg caccgctgtt
     5701 cgccgggcgt cacgtcagcc aattgcactg gggcggcgga acgccgacgt atctgaataa
     5761 agcgcaaatc agccgcctga tgaagctgct gcgcgaaaac ttccagttca atgccgatgc
     5821 ggagatttcg atcgaagtcg atccgcggga aatcgaactg gatgtactcg atcatttacg
     5881 cgccgaaggc tttaatcgcc tgagcatggg cgtgcaggac ttcaacaaag aagtgcaacg
     5941 tctggttaac cgcgagcagg atgaagagtt catctttgca ctgcttaacc atgcgcgtga
     6001 gattggtttt acctccacca acatcgacct gatttacggc ctgccgaaac agacgccgga
     6061 gagtttcgcc tttaccctga aacgtgtggc ggagctgaac cccgatcgtc tgagtgtctt
     6121 taactacgcg catctgccga ccatttttgc tgctcagcgc aaaatcaaag atgctgacct
     6181 gccgagtccg cagcaaaaac tcgatatcct gcaggaaacc atcgccttcc tgacgcaatc
     6241 gggctatcag tttatcggta tggatcactt tgcccgtccg gatgacgagc tggcggtggc
     6301 ccagcgtgaa ggcgtgctgc atcgtaactt ccagggctac accactcagg gcgataccga
     6361 tctgctgggg atgggcgttt ccgccatcag catgattggc gactgctacg cgcagaacca
     6421 gaaagagttg aagcagtact atcagcaagt ggatgaacaa ggcaatgcgc tgtggcgtgg
     6481 tattgcgcta acgcgtgatg actgtattcg ccgcgatgtg attaagtcgc tcatctgcaa
     6541 cttccgtctg gattacgccc ctattgagaa acagtgggat ttgcacttcg ctgattactt
     6601 tgcggaagat ctcaagctgc tcgccccgtt agcaaaagat gggctggtgg atgtggatga
     6661 gaagggaata caggtgacgg cgaaaggtcg cttgctgatc cgcaacattt gcatgtgctt
     6721 tgatacctat ctgcgccaga aagcgcggat gcagcagttc tcacgggtga tttaaatagt
     6781 gaatggcgct tcgtttacaa agtagaaaac gaaagcgcca tcaatgtgct gagaacaaga
     6841 ttgcctgatg cgctacgctt atcaggccta caaaacctgt tgaaaatatt gcttttgtag
     6901 gccggataag gcgttcacgc cgcatccggc atgaacaaag agcacttcat cagcaatctg
     6961 aaactcaact aaacagccca atcattgcgg cacacagcac ggcagcaacg gcagtttgcg
     7021 gtgtgtggct gtcaactgcg ccactcgata ccagattaat tattgattcc agcataatga
     7081 ctctccccgt tttccgggca agatcatact gaacttatcg gaacagtaaa gcgtaaaata
     7141 ccagcaattt gcgctcaata atcaatcttt acacacaagc tgtgaatcac tccatcccca
     7201 gctcttttaa cttacgcgtc agggtgttgc ggccccagcc gagtagccgc gccgcttcct
     7261 gtttatgccc ctgcgtatgt cgcaacgcgg tcgtcagtaa cgtccgctcc agctctggtt
     7321 gcgcttcgga aagcagattt tgatgaccgg aacgcagcgc tctgtctgcc cactgcgcaa
     7381 gaagcgttgc ccagctgtcc ggttgcattt gcgaagtact ctccgcaacc gttgattcaa
     7441 acagttcgcc gggcaaatcc tgaatcaaca cttcctgccc ggcggccatc accgttagcc
     7501 agcggcaggt gttttccagc tggcgcacgt tgcctggcca cgccagacgc gtcagagcag
     7561 cttcggtttc cggatgcagt aacttcgctt ctacgcccag ttcgcgcgcg gcaacctgta
     7621 aaaaatggcg cgccagacgg ggaatatctt cccgacgttc gcgcagcggc ggcagatgaa
     7681 cgcggataac gttcaggcgg tggaacagat cctcacggaa cttaccttcc tgcactcgct
     7741 gttcgagatt ctggtgagtg gcagcgataa tacgcacatc cactttcacc ggcgcatagc
     7801 cgccaacgcg gtaaaactga ccgtctgcca gcacgcgcag caaacgcgtc tgcacatcca
     7861 gcggcatatc accaatttcg tcgaggaata atgtaccgcc atcggcctgt tcaaaacgcc
     7921 cctgacgaat ggtattcgcg ccagtaaacg cgcctttctc gtggccaaac agttctgatt
     7981 cgatcaaatc ttttgggata gctgccatat tcagcgcgat aaacggcgct ttggcgcgcg
     8041 gactgtggcg atgcagggca tgagcgacca gttctttacc ggtgccggat tcgccgttaa
     8101 tcagcacgct aatagaagaa cgcgaaagcc gaccgataat acggaacacg tcctgcatgg
     8161 ctggcgcttc gccgatgata tcggtcgttg ggccgttaag ctgaatatta cgcggctgct
     8221 gctgttcctg gtaatgactg atagcgcgct caaccagcgc cactgcttcg tcgatatcaa
     8281 acggtttggg cagataatca aacgcccctt gttgataggc gctgacggca gcatccagat
     8341 cggaatgtgc ggtcataatg atgaccggaa gcattggatg gcgctgttta atctgcttga
     8401 gcagcgccag cccgtccatt cccggcatac ggatatctga aagcagcaca tccggcgttt
     8461 tgctcgccag cgcctccagt acttccgcgc cgttctcaaa tgtcgtacag gttaaacccg
     8521 ctccagcgag cgcacgttca agcacccaac ggatggaact atcgtcatcg actacccaga
     8581 ctatccctcg ttgcataaac gtcaccttta tttcctgata ggcaggtaaa ccgagaactc
     8641 ggtatgacct ggccaactgg taaattcaat tttgcctgaa tgctgatcaa tcaaattacg
     8701 ggcgatggat aagccaagcc cggtgccacc ttcgcggccg ctgaccatcg ggtaaaacag
     8761 cgtatcctgc aaatgaggcg gaatgcccgg cccgttatct tccacatcaa tccgcgccgc
     8821 cagccggtag cgctcgccgt gtaaggtcag ttgaaacgcg gtgcgggtac gcagaatgat
     8881 ttcaccgcct tccggcccca gcgcctgtag cgcattgcgc acaatattca gcaagacctg
     8941 ttcaatttga tccgggtcgt gcgccagttc cggcaggctg gggtcgtaat cacgaatcaa
     9001 ccgcacgttg tccggcagtt ccatcgacac cagcgtcacc acgcgttcag ctactttgtg
     9061 aatactttcg gtaacgcgcg taccgggcag ctgcggcccc aacagacggt cgaccagatt
     9121 tcgcagccgg tccgcctgtt cgataatcac tttggtatat tcgagtagtg atgggtcagg
     9181 taacgctttg ctgagcagct gcgccgcgcc acgtaaacct ccaagcggat ttttaatctc
     9241 atgtgccagg ccgcgcacta aatcacgggc agcaacctgc tgggcgtgct gtagctgttc
     9301 ctgacttaag cggcgctggt tatccatcgg agccatctcc agcaggatca tgccgtccgg
     9361 catacgctgg gccgtcacag aaaggatatg cgagcgcccg tcgatgacca gcgtcacttc
     9421 gttatcggta aaaccttgcc ccgcctccag actttcttgc atcagctcga tatttaatga
     9481 gaagtagctc aacagttccg gtaacggtgt accaaacaat ttgcgggagc tttgggcgag
     9541 cagttgttgc gcggcggggt tggcgtaatg gatcgccaga ttgtcatcaa ttaacaaaat
     9601 actgttaatc agcgagttga ggatctgccc agcatcgggc tgcgtgcctg ttgccataaa
     9661 gcagtctcct gaacaggttg caccatttta gtgcattata gctttttacg gataaaaagc
     9721 gcgaagcatc agagaattga cggagaaaaa agcccatgca gagatgggct gaaagtttcc
     9781 acggcaacta aatcccggcg ttgttgcgcc gggtagtaca acttcaacta ttagacgctg
     9841 tagtacagct caaactctac cggatgcgga gtcatacgca cgcggtcatc ttcttcgcga
     9901 cgtagagcga tgtacgcatc aattgcttcg tcagtgaaca cgccaccggc tttcaggaac
     9961 tcgcggtcca gatccagttc gttcagtgct tcttccagag agcctgcaac ctgtgggatc
    10021 tctttcgctt cttctggcgg caggtcatac aggtttttgt ccatggcttc gcccggatgg
    10081 atcttgttct tgataccatc aagaccggcc atcagcaggg cagcaaagca caggtacggg
    10141 ttagctgccg gatccgggaa acgtacttcg atacgacgtg ctttcggaga agaaaccacc
    10201 ggaatacgga tagacgcaga acggttacgc gcagagtaag ccagcattac cggtgcttca
    10261 tagcccggga ccagacgctt ataagagttg gtggtcgggt ttgccagggc gttaatcgct
    10321 ttagcgtgtt tgattacgcc gccaatgtag tacagcgcct gctcagacag acctgcgtat
    10381 ttgtcgcctg cgaacaggtt aacgccgttt ttagacagag acatgtggca gtgcataccg
    10441 gagccgttat caccgaacat cggttttggc ataaaggtcg cggttttacc gaagcggtgc
    10501 gctacgttgt gcacaacata tttgtagatc tgaatttcgt cagctttttt ggtcatggta
    10561 ttgaagcggg tagccacttc gttctgacca gcagtcgcta cttcgtggtg atgggcttca
    10621 accaccagac ccatctgttc catcaccaga cacatttcag aacgaatatc ctgagccgag
    10681 tctaccggtg gaaccgggaa gtaaccgcct ttcactgccg gacggtgacc tttgttacca
    10741 ccttcgtatt gggtggagga gttccatgcg ccttcgatat cgtcgatagc aacgtgggaa
    10801 ccggagatag atgatccgaa acggatgtca tcgaacagga agaattcagg ttctggcccg
    10861 aacagtacgg tgtcggcaat gccagtggaa cgcaggtaat cttcggcgcg cttcgcaatg
    10921 gagcgcgggt cacggtcata gccttgcagg gtgccaggtt caaggatgtc gcaacggata
    10981 atcagggtgg agtcggcgaa gaacgggtca atcactgcgg tggatgcgtc tggcatcagc
    11041 accatgtcgg actcgttaat gcctttccag ccgccaatcg aggagccgtc aaacattttg
    11101 ccttcttcga agaattcagc attcacctga tgagcaggga tagtgacgtg ctgttcttta
    11161 cctttagtat cggtgaagcg caaatcaaca aacttcactt cgtgctcgtt cagcatcgtc
    11221 agtacgtgtt cagcggacat actttaactc tcctggattg gtcatggtcg tcgtggtaac
    11281 gaaatctgca attattttgg ccgtgtcgcc gtaaaaaaga taaagcgaaa tctgtgccaa
    11341 cttttaaatt gcccctaaaa ggcgttatca tgcgcaccat cgtgcaaaag ggctgcacca
    11401 cgatgtgaat gttgcaccaa tatagtgctt caatggaaac attaagcacc atgttggtgc
    11461 aatgaccttt ggataaccct ttttatgctc cgtgaaagcg atcacaaagg gactctgcaa
    11521 tacttgtttg cggaggatgt ttgtgatcct gttttgtagt gcgattaatc cgtgtacaat
    11581 aacgcgctat ttctaatgcc tgaggcaaag ttgtgatcga aaaattgcgt aatatcgcca
    11641 tcatcgcgca cgtagaccat ggtaaaacca ccctggtaga caagctgctc caacaatccg
    11701 gtacgttcga ctctcgtgcc gaaacccaag agcgcgtgat ggactccaac gatttggaga
    11761 aagagcgtgg gattaccatc ctcgcgaaaa acaccgctat caaatggaat gattaccgta
    11821 tcaacatcgt tgataccccg gggcacgccg acttcggtgg tgaagttgaa cgtgtaatgt
    11881 ccatggtaga ctcagtgctg ctggtggttg acgcatttga cggcccgatg ccgcaaacgc
    11941 gcttcgtaac caaaaaagcg tttgcttacg gcctgaagcc gattgttgtt atcaacaaag
    12001 ttgaccgccc tggcgcgcgt cctgattggg ttgtggatca ggtattcgat ctgttcgtta
    12061 acctcgacgc gaccgacgag cagctggact tcccgatcgt ttacgcttct gcgctgaacg
    12121 gtatcgcggg tctggaccac gaagatatgg cggaagacat gaccccgctg taccaggcga
    12181 ttgttgacca cgttcctgcg ccggacgttg accttgacgg tccgttccag atgcagattt
    12241 ctcagctcga ttacaacagc tatgttggcg ttatcggcat tggccgcatc aagcgcggta
    12301 aagtgaagcc gaaccagcag gtcactatca tcgatagcga aggcaaaacc cgcaacgcga
    12361 aagtcggtaa agtgctgggc cacctcggtc tggaacgtat cgaaaccgat ctggcggaag
    12421 ctggcgatat cgtggcgatc acgggccttg gcgaactgaa catttctgac accgtttgcg
    12481 acacgcaaaa cgttgaagcg ctgccggcac tctccgttga tgagccgacc gtttctatgt
    12541 tcttctgcgt taacacctcg ccgttctgcg gtaaagaagg taagttcgta acgtctcgtc
    12601 agatcctgga tcgtctgaac aaagaactgg tacacaacgt tgcgctgcgc gtagaagaaa
    12661 ccgaagacgc cgatgcgttc cgcgtttctg gtcgtggcga actgcacctg tctgttctga
    12721 tcgaaaacat gcgtcgtgaa ggtttcgaac tggcggtatc ccgtccaaaa gtaatcttcc
    12781 gtgaaatcga cggtcgtaaa caagagccgt atgaaaacgt gactctggac gttgaagaac
    12841 agcatcaggg ttctgtaatg caggcgctgg gcgaacgtaa aggcgacctg aaaaacatga
    12901 atccagacgg taaaggccgc gtacgtctcg actacgtgat cccaagccgt ggtctgattg
    12961 gcttccgttc tgagttcatg accatgactt ccggtactgg tctgctgtac tccaccttca
    13021 gccactacga cgacgtacgt ccgggtgaag tgggtcagcg tcagaacggc gtactgatct
    13081 ctaacggtca gggtaaagcg gtcgcgttcg cactgttcgg tctgcaggac cgcggtaagc
    13141 tgttcctcgg tcacggtgca gaagtttacg aaggtcagat tatcggtatt cacagccgct
    13201 ctaacgacct gactgtaaac tgcctgaccg gtaagaaact gaccaacatg cgtgcttccg
    13261 gtactgacga agctgttgtt ctggttccgc ctatccgcat gactctggaa caagctctgg
    13321 agttcatcga tgatgacgaa ctggtagaag tgactccgac ctctatccgt attcgtaaac
    13381 gtcacctgac ggaaaacgat cgtcgtcgcg ccaaccgcgc accgaaagac gattaatttc
    13441 gttttttagt tataaaaaac ctgccatcgc t
//
LOCUS       DAKISA010000114        13263 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.114, whole genome shotgun sequence.
ACCESSION   DAKISA010000114 DAKISA010000000
VERSION     DAKISA010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13263
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.114"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(894..1985)
                     /gene="ychF"
                     /locus_tag="OVW45_003493"
     CDS             complement(894..1985)
                     /gene="ychF"
                     /locus_tag="OVW45_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCV5527855.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRVGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(2102..2686)
                     /gene="pth"
                     /locus_tag="OVW45_003494"
     CDS             complement(2102..2686)
                     /gene="pth"
                     /locus_tag="OVW45_003494"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCV5527856.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2963..3241
                     /gene="ychH"
                     /locus_tag="OVW45_003495"
     CDS             2963..3241
                     /gene="ychH"
                     /locus_tag="OVW45_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCV5527857.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(3296..4975)
                     /gene="dauA"
                     /locus_tag="OVW45_003496"
     CDS             complement(3296..4975)
                     /gene="dauA"
                     /locus_tag="OVW45_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCV5527858.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(5100..6047)
                     /gene="prs"
                     /locus_tag="OVW45_003497"
     CDS             complement(5100..6047)
                     /gene="prs"
                     /locus_tag="OVW45_003497"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCV5527859.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(6198..7049)
                     /gene="ispE"
                     /locus_tag="OVW45_003498"
     CDS             complement(6198..7049)
                     /gene="ispE"
                     /locus_tag="OVW45_003498"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCV5527860.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(7049..7672)
                     /gene="lolB"
                     /locus_tag="OVW45_003499"
     CDS             complement(7049..7672)
                     /gene="lolB"
                     /locus_tag="OVW45_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCV5527861.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7886..9142
                     /gene="hemA"
                     /locus_tag="OVW45_003500"
     CDS             7886..9142
                     /gene="hemA"
                     /locus_tag="OVW45_003500"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCV5527862.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            9184..10266
                     /gene="prfA"
                     /locus_tag="OVW45_003501"
     CDS             9184..10266
                     /gene="prfA"
                     /locus_tag="OVW45_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCV5527863.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            10266..11099
                     /gene="prmC"
                     /locus_tag="OVW45_003502"
     CDS             10266..11099
                     /gene="prmC"
                     /locus_tag="OVW45_003502"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCV5527864.1"
                     /translation="MEYQYWLREAISQLQASESPRRDAEILLEYVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            11096..11488
                     /locus_tag="OVW45_003503"
     CDS             11096..11488
                     /locus_tag="OVW45_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCV5527865.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11492..12301
                     /locus_tag="OVW45_003504"
     CDS             11492..12301
                     /locus_tag="OVW45_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCV5527866.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            12337..13191
                     /gene="kdsA"
                     /locus_tag="OVW45_003505"
     CDS             12337..13191
                     /gene="kdsA"
                     /locus_tag="OVW45_003505"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCV5527867.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
BASE COUNT         3347 a         3286 c         3395 g         3235 t
ORIGIN      
        1 attcagcgag acgatctgct ttggtattcc cattgtgttg tttgatgccc ataccactcc
       61 tatatagtac ccagactatg ggtctatgga ttttgtcatt ttgtttaaat caatgaataa
      121 tatcctctta tcatcatgat atttgtatgg tgcaatcatg atgagcttct cagagaataa
      181 ttctctatta ccgggcaaaa tcaggaacac aggaattaat atatatttaa catccccctt
      241 acaaaggtga gtttatatcg agttattccc taagttgtcg ttacttattc caattcgtta
      301 ctataaatat ggcaaaaata ttacaacaaa cagcggttaa aaaatttggt gaagattttt
      361 tgtaataatc ctaattagtt atgggaattt cgcttgatgt atctgcataa caaaaacaat
      421 atgcgccatt tttgcattac taataagaaa aagcaaaaaa catcgatatt catttttggt
      481 caatgggttt tctggcctat cgttgttatt gtgctactgg ttatcatttt catcttgcca
      541 ggatcgcacg taatcacgtt attcggggac tttatctgcg ggatattacc cttcaggtaa
      601 gtccttttct cgccagcata ttggagcgtg atcaatattg atcagctgtg aagggggtga
      661 tgtgtagttg aacaaaaaat tacgttatat cactactaat gagatatccg tcatctgaca
      721 ctgatgtctt taattattct cgtggttcac tgtaggcaat aagcacaaaa gtgtaggatg
      781 ttacaagaat gattaggact cggtgaaatg aaaaatccac gcaattgcgt ggattttata
      841 tacttttgcg ctcttcatga gatttagcga aacctcatga gacaataaat taattagacg
      901 ttgaaaagga agttcatcac atcgccatct ttaacgatgt aatctttacc ttctgcacgc
      961 attttgcctg cttctttcgc gccttgttca cctttgtaag tgatgaaatc ttcaaacgag
     1021 atggtttgtg cacggataaa gcctttttca aaatcagtat ggattttgcc cgctgcctgc
     1081 ggcgcggttg ctccaaccgg aatggtccat gcacgcactt ctttcacccc agcggtgaag
     1141 taagtttgca ggttcagcag cttataaccg acacggatca cacggttcag gcccggttct
     1201 tccagcccaa gctcctgcat aaactcgtca cgttcttcgt cgtccagttc ggcaatgtct
     1261 gcttcaacag cagcacaaac cggaaccaca acagaacctt ctttcgccgc gatttcacgc
     1321 acctggtcaa gatatgggtt gttttcaaaa ccgtcttcgt tgacgttggc gatgtacatt
     1381 gttggtttca gcgtcaggaa gctcaggtaa cgaatagccg ctttctcttc agcgcttaaa
     1441 tccagcgcgc gcagcatacc tgcgttttcc agctggggca ggcatttttc caggaccgcc
     1501 agctcagctt tcgcgtcttt atcgccacct ttggctttct tctgtacgcg atgaatcgca
     1561 cgttcgcagg tgtcgaggtc tgccagcgcc agttcggtgt tgataacttc aatatcgtca
     1621 gccgggttaa ctttgccgga aacgtgaatg atgttgtcat tttcaaagca gcgaacaacg
     1681 tgaccgatcg cttcggtttc acggatgttg gtcaggaact ggttacccag accttcgcct
     1741 ttcgatgcgc cttttaccag accggcgata tcgacaaatt ccatggtcgt gggaagcgta
     1801 cgttgcggtt ttacgatttc agccagttga tccaggcgag ggtcaggcat tggtacgacg
     1861 cctgtgttcg gctcaatggt gcagaatgga aagttggccg cttcaatacc ggctttggtc
     1921 agcgcgttga acagggtaga tttcccgacg ttgggcaaac cgacgatacc gcatttgaat
     1981 cccatgattt aaatcacctt aatatcttaa taatcaacct gttattgcta acagattgca
     2041 gaaatggaaa taactttgcc tattatacac ggcactcggc aaaaatgccg cagacaacga
     2101 cttattgcgc tttaaaggcg tgcaatcggt tcgttgcttt ggtcaagcca tctgtaaacc
     2161 acatttcagt acaacgcgcc gcttcgtcga tggcttcatc aattaacttc tgttcactaa
     2221 caggcggttt gcctaacaca aaaccgacaa ctttattttt atcgccagga tgaccgattc
     2281 cgatgcgtaa acggtgaaag ttagggttat tacccaattt actgatgatg tctttcagtc
     2341 cattgtgacc accatggcca ccgcccaatt taaatttggc gacgccagga ggcagatcca
     2401 gttcgtcgtg ggccaccaga atttcgtccg gattaatgcg gaaaaaactg gccatcgccg
     2461 caacggcttt gccgctgaga ttcataaatg tagtcgggac taacaggcgg acatcttcgc
     2521 ctccaagagt gactcgcgaa gtataaccaa agaatttagc ctcttcgcgc agcggagcgc
     2581 gcaaacgctc tgccagtaag tcaacgaacc aggcaccagc attatgtcgc gttgcggcgt
     2641 attcagcacc ggggttcgcc aggccgacaa tcaatttaat cgtcacgttt tttgtcctga
     2701 gtgtgtacat aactggcgcg tagtttactg gttgcggccc cgcttgacaa aaaactgcgt
     2761 atcaaatgca gataacgtaa taattgcctg agtggactat tagaaagtca aggtgttcag
     2821 gcgtttattt gtaaagtttt gttgaaataa gggttgtaat tgtgatcacg cccgcacata
     2881 acccacaggg tgttgtctat actttacaca taaggaagag gggtattccc tgttacaacc
     2941 cagaaagttc cggaggtgac atatgaaacg caaaaacgct tcgttactcg gtaacgtgct
     3001 catggggttg gggctggtgg taatggtggt cggcgtgggg tattcaatcc tcaaccagtt
     3061 accacagttt aatatgcccc agtatttcgc acatggtgca gtgctaagta ttttcgtcgg
     3121 tgccattctc tggctggcgg gtgcccgtgt tggcgggcat gaacaggtgt gcgaccgtta
     3181 ctggtgggtt cgtcactatg acaaacgttg ccgccgtagc gataatcgcc gtcatagcta
     3241 acaaataatg cagtttgctg accagtcagt ttgcgctgac tggtcaatct cgtacttata
     3301 aatccgccat cgccgcgcga cgattcggga agaacgcgag gcgtcccggg atcggttgaa
     3361 tgcctgcgcg agccatagtg cgcagtggct ggaattccac gttgcacacg cgcagttcac
     3421 atccttcggg cagacgcttc acaaaacgct ggaacgcatc aagaccacca gcatcaagta
     3481 ccggaacggc atcccacttc agaatcacaa tccgtttgcc ttcaagacgt gactccaggt
     3541 ccgtgaacaa gccttcagca gcagcaaaaa acagcgggcc aataacgcgc agtaccagaa
     3601 catcgtctgg aacatctacg actaccggtg ccaggcgagt catacgtgcg atacgacgca
     3661 taaacagcag cgatgccagc acgatcccca cgctgatggc aataaccata tcaaacagca
     3721 cggtcagcga catgcacagc agcatgacaa tgatgtcatc tttcggtgca tgacgcagca
     3781 agtcgaccac tttatgcgct tcactcatgt tccacgccac catcaacagc agggctgcca
     3841 tagcggaaag cggcagccag gagagcagcg gtgccagtac cagcagggca agaataacca
     3901 gaatagagtg gatcaccgcc gagatagggg aagttgcccc ggcacggacg ttagcggcag
     3961 aacgcgcgat ggcagctgta gcggtaatac caccaaagaa cggagcgata atattcccca
     4021 gtccctgtcc aaccagttcg ctgttcgcct tgtgtttcgt cccggtcata ccatccagta
     4081 ccacggcgca gagcagagat tcgattgcgc cgagcattgc cattgagaat gccgcaggca
     4141 gcagtgtgcg aatagaatcc caggttagcg tgaattctga attaggcaga tcccacggca
     4201 gcaccagttg cggcagcagt tgcggaatac cgttaccctg agaaccatcg gccaggacgt
     4261 agtggaattg cgaaccgatg gtagcaacat gtccgccgag caggttaaca atccccatca
     4321 ccgcgcaacc agccagcaag gccggaaggt gaccgggtaa acgaatgccc agacgcggcc
     4381 aaaaaacaag aatacctagc gtcacaatgc caatggcagc atcacccaca ttaatggtcg
     4441 gcagcgccat aaataatgcg ccgacttttt gtagataatg ttccgggaca tgggccattt
     4501 gcagaccgag aaaatcttta atctgcatgg taccgatggt gatcccgata cccgaggtga
     4561 aacctaaggt gacggaaacc ggaatatact caatcaggcg accaaagcgt gccagaccca
     4621 tcagaatcaa aaagatcccc gacagcaagg tcgcaaccag cagtcctgcc agtccaaact
     4681 gttgcgacac gggatagaga attaccacaa atgccgcagt cggaccggaa acgctaaagc
     4741 gtgacccacc cgtcagagca atgacaatcc ccgcaacagc tgcggtatat aaaccgtact
     4801 ggggtgccac accactacca atagccaacg ccatcgccag cgggatagca ataatcccga
     4861 cggttatccc ggcaatcagg tcacgggtaa accgtgcggc agtatatttt tctttccagc
     4921 aagcgtcgat cagagcgcgg aaaggcatca catgtgagga aaatattttg ttcacaataa
     4981 tgtttcatcc gtgagcgcat catctgtcaa ctaaatggca ggtgaaggag gcataggtca
     5041 tacaaatgga tattacagac aaaaaaaccc gccgcagcgg gtctttgagc cgggttcgat
     5101 tagtgttcga acatggcaga gatcgattct tcgttgctga tacgacgaat cgcttcggcc
     5161 agcatacctg acagggtcag agtacgcacg ttcggcagtg atttgatttc atcgctcagc
     5221 ggaatggtat cgcagacaac gacttcatca attacagagt tacgcaggtt gttcgccgcg
     5281 ttgccagaga agatcgggtg agtcgcgtac gcaaatacac gtttagcacc acgttctttc
     5341 agagcttcag cagctttaca cagcgtaccg ccagtgtcga tcatatcatc gaccagtacg
     5401 cagtcacgac ctgcaacgtc accgatgata tgcatcacct gggaaacgtt cgcacgcgga
     5461 cgacgtttgt cgatgattgc catatcggta tcgttcagca gcttagcgat agcgcgggca
     5521 cgcacaacgc cgccgatgtc cggagaaacc acaattgggt tatccagatt cagctgcagc
     5581 atgtcttcca gcaggatcgg gctaccaaat acgttatcaa ccggaacgtc gaagaaaccc
     5641 tgaatctgtt cagcgtgcag atccactgtc agcacacggt caacaccgac gctggagagg
     5701 aagtctgcaa ccactttcgc agtgattggt acacgagcgg aacggacgcg acggtcctgg
     5761 cgcgcatagc caaagtaggg gataacagcg gtgatacgac ctgcggaagc acgacgcagg
     5821 gcatcaacca taacgactaa ttccatcagg ttgtcgttag taggggcaca agtggactgg
     5881 atgatgaaaa tatcaccacc gcgtacattt tcgttgattt gtacactgac ttcgccgtcg
     5941 ctaaagcgac ctacagcggc gtcgccgagt gaagtgtaca ggcggttggc aatacgttgt
     6001 gctagttccg gggtggcgtt accagcaaaa agcttcatat caggcacgag aagaacctca
     6061 ggcatgcgtc cattggtgga aagaatctgc cgaaaactgt gcgggccagg catgatcctt
     6121 tccaggcggt gtattaaaga gcgcgatgca acgtctggaa caaggtgacg ttgtcaccga
     6181 aactcagctt gcccggctta aagcatggct ctgtgcaatg gggaaagatt aacacctttc
     6241 gccacaaagc cattgagcca ttccggggct tgctctagca cctggcgggc ttcagactct
     6301 gtatcaaatt cagcaaagac acaggcccct gtcccagtca ggcgcgacgg ggcgtattct
     6361 aacagccagg aaagcaccgc atcaacctcg cgaaaacgtt ttcttgcgat aacctcgcaa
     6421 tcattgctga attcacattt tagcaacgtt tctattgacc tttttggcgt attgcgcggg
     6481 agttcaggat ctttaaaaat cactggtgtc ggaatactta caccagggtg cgccaccaga
     6541 taccacttct ctggcggatc caccggcgtt agtatttcac caacgccttc ggcaaacgcg
     6601 gcatgccccc gaacaaagac aggaacatct gcgcccagcg tcagccccat ttccgccagc
     6661 tcatccatgc ttagcccgca ttgccagaga tgatttaatg ccaccaggac cgtcgcggca
     6721 ttggatgaac caccgccgag accgccgccc atcggcaaac gcttgtcaat gctgatattc
     6781 gcaccgcttc ccgtcggaag acgcccgctg tctgccgcag ttttcatcaa caaccgcgct
     6841 gcgcgaacga tcaggttatc ttcatgttcc acgccttcaa cgggcgttaa cagacgaata
     6901 tccccatcgt cacgaagctc aatgctgatg gtgtcgccgt aatcaagaaa ctgaaacagc
     6961 gtttgcagcg tgtggtaacc atccgcacgc tgaccggtaa tgtataaaaa cagattaagt
     7021 tttgccggag agggccactg tgtccgcatt atttcactat ccagttatcc atttttaact
     7081 tgatgcgttg accaccgtcg gtgagttcca tattggctgg catcgcaggt tgcgttttgg
     7141 tgtcataacc accataaaca accttccagt ttttgccatt ctggctgtag gtaatttcgc
     7201 tcaggcggta ctggtcgtcc agtttgtagt cggttgcatc acccggtaaa cctaaaatcc
     7261 attggcgcaa gctgttgagc ggaattggca ttccggtcaa tttgccaatc atctcttcgg
     7321 cgtcatcggc ggtataacgc tgacctttat tgtcgactaa ctgcacgtta cccggttgag
     7381 cattcagctc cagttccgtg ctgcccaatg ggttagtgag cagcagacgg tagcgatcct
     7441 ggccggtttg ctgccagaaa aagcgggcgt acactttttg ttggtcagaa atataagcga
     7501 acgcgccgcg agtctgatac tgattaagat tgcgcacgtc ttgctgatgc tgacgccatt
     7561 gtggcgaatc cgggcttttg ccaggacctt tgggcgtggt aacggaacag gcagtgagca
     7621 caagagcagc cagcggtagc aggcggataa gacgaaaatc gggcaggggc atagtgatga
     7681 caagtccttg agatacgttg cagttataac ccttaatgct agcgttaccg tccgctatcg
     7741 tctatgttca agttgtctta attgccagaa tctaacggct ttcggcaatt actccaaaag
     7801 ggggcgctct cttttattga tcttacgcat cctgtatgat gcaagcagac taaccatatc
     7861 aacgttggta ttatttcccg cagacatgac ccttttagca ctcggtatca accataaaac
     7921 ggcacctgta tcgctgcgag aacgtgtatc gttttcgccg gataagctcg atcaggcgct
     7981 tgacagcctg cttgcgcagc cgatggtgca gggcggcgtg gtgctgtcga cgtgcaaccg
     8041 cacggaactt tatcttagcg ttgaagagca ggataacctg caagaggcgt taatccgctg
     8101 gctttgcgat tatcacaatc ttaatgaaga agatctgcgt aaaagcctct actggcatca
     8161 ggataacgac gcggttagcc atttaatgcg tgttgccagc ggcctggatt cactggttct
     8221 tggggagccg cagatcctcg gtcaggttaa aaaagcgttt gccgattcgc aaaaaggtca
     8281 tatgaaggcc agcgaactgg aacgcatgtt ccagaaatct ttctctgtag cgaaacgcgt
     8341 tcgcactgaa acagatatcg gtgccagtgc tgtgtctgtc gcttttgcgg cttgtacgct
     8401 ggcgcggcag atctttgaat cgctctctac ggtcacagtg ttgctggtag gcgcgggcga
     8461 aaccatcgag ctggtagcgc gtcatctgcg cgaacataaa gtacagaaga tgattatcgc
     8521 caaccgcact cgcgaacgtg cccaaattct ggcagatgaa gttggcgcgg aagtgattgc
     8581 cctgagtgag atcgacgaac gtctgcgcga agccgatatc atcatcagtt ccaccgccag
     8641 cccgttaccg attatcggga aaggcatggt ggagcgcgca ttaaaaagtc gtcgtaacca
     8701 accaatgctg ttggtggata ttgccgttcc gcgcgatgtt gagccggaag ttggcaaact
     8761 ggcgaatgct tatctttata gtgtggacga tctgcaaagc atcatttcgc acaacctggc
     8821 gcagcgtaaa gccgcagcgg ttgaggcgga aactattgtc gctcaggaaa ccagcgaatt
     8881 tatggcgtgg ctgcgagcac aaagcgccag cgaaaccatt cgcgagtatc gcagccaggc
     8941 agagcacgtt cgcgatgagt taaccgccaa agcgttagcg gcccttgagc agggcggcga
     9001 cgcgcaagcc attatgcagg atctggcatg gaaactgact aaccgcttga tccatgcgcc
     9061 aacgaaatca cttcaacagg ccgcccgtga cggggataac gaacgcctga atattctgcg
     9121 cgacagcctc gggctggagt agcagtacat cattttcttt ttttacaggg tgcatttacg
     9181 cctatgaagc cttctatcgt tgccaaactg gaagccctgc atgaacgcca tgaagaagtt
     9241 caggcgttgc tgggtgacgc gcaaactatc gccgaccagg aacgttttcg cgcattatca
     9301 cgcgaatatg cgcagttaag tgatgtttcg cgctgtttta ccgactggca acaggttcag
     9361 gaagatatcg aaaccgcaca gatgatgctc gatgatcctg aaatgcgtga gatggcgcag
     9421 gatgaactgc gcgaagctaa agaaaaaagc gagcaactgg aacagcaatt acaggttctg
     9481 ttactgccaa aagatcctga tgacgaacgt aacgccttcc tcgaagtccg agccggaacc
     9541 ggcggcgacg aagcggcgct gttcgcgggc gatctgttcc gtatgtacag ccgttatgcc
     9601 gaagcccgcc gctggcgggt agaaatcatg agcgccagcg agggtgaaca tggtggttat
     9661 aaagagatca tcgccaaaat tagcggtgat ggtgtgtatg gtcgtctgaa atttgaatcc
     9721 ggcggtcatc gcgtgcagcg tgttcctgct acggaatcgc agggtcgtat tcatacttct
     9781 gcttgtaccg ttgcggtaat gccagaactg cctgacgcag aactgccgga catcaaccca
     9841 gcagatttac gcattgatac tttccgctcg tcaggggcgg gtggtcagca cgttaacacc
     9901 accgattcgg caattcgtat tactcacttg ccgaccggga ttgttgttga atgtcaggac
     9961 gaacgttcac aacataaaaa caaagctaaa gcactttctg tacttggtgc tcgtatccac
    10021 gctgcagaaa tggcaaaacg gcaacaggcc gaagcgtcta cccgtcgtaa cctgctgggg
    10081 agtggcgatc gcagcgaccg taaccgtact tacaacttcc cgcaggggcg cgttaccgat
    10141 caccgcatca acctgacgct ctaccgtctg gatgaagtga tggaaggtaa gctggatatg
    10201 ctgattgaac cgattatcca ggaacatcag gccgaccaac tggcggcgtt gtccgagcag
    10261 gaataatgga atatcaatac tggttacgtg aagcaataag ccaacttcag gcgagcgaaa
    10321 gcccacgccg tgacgctgaa atcttattgg agtatgtcac tggcaggggg cggactttta
    10381 ttctggcctt tggtgaaacg cagctgactg acgaacaatg tcagcaactt gatgcgctac
    10441 tgacacgtcg tcgcgatggt gaacccattg ctcatttaac cggggtgcga gaattctggt
    10501 cgttgccgtt atttgtttcg ccagcgacct taattccgcg cccggatacg gagtgtctgg
    10561 tggagcaggc actggcgcgg ttgcctgaac aaccttgccg tattctcgat ctcgggacgg
    10621 gtaccggggc gattgcgctt gcgctggcta gcgagcgccc ggactgcgaa attatcgctg
    10681 tagatcgtat gcctgatgct gtctccctgg cacaacgtaa tgcccagcat ctggcgatca
    10741 aaaatatcca cattctgcaa agcgactggt ttagcgcgct agccgggcag cagtttgcga
    10801 tgattgtcag caatccgccg tatattgacg agcaggaccc acatcttcaa caaggcgatg
    10861 tccgctttga gccgctcact gcgctggttg cggcagacag tggaatggca gacatcgtgc
    10921 atatcatcga acagtcgcgt aacgcgctgg tatccggcgg ctttctgctt ctggaacatg
    10981 gctggcagca gggcgaagcg gtgcgacaag catttatcct cgcggggtat catgacgtcg
    11041 aaacctgccg tgactatggt gataacgagc gcgtaacgct cggccgctat tatcaatgac
    11101 aagtttttct acactgctta gtgttcatct tattagtatc gcgctttctg ttgggctatt
    11161 aaccttacgt ttctggctac gttatcagaa gcatccacag gcatttgctc gctggacgcg
    11221 cattgtgccg ccggttgtcg atacggtgtt attgttaagc ggcattgcgt tgatggctaa
    11281 agcgcacatc ctgccatttt ccgggcaggc acagtggctg actgaaaagc tgtttggagt
    11341 tatcatttat atcgttttgg gttttattgc actcgattat cgtcgtatgc acagtcagca
    11401 ggcgcgcatt attgccttcc cgctggcgtt ggtggtgctg tacatcatca ttaaactcgc
    11461 caccacaaaa gtaccgttac tggggtaagt catgagatcg ttagctgatt tcgaatttaa
    11521 taaagcgcca ttgtgcgaag gcatgatcct ggcttgcgaa gcaatccgcc gcgattttcc
    11581 ctcgcaagat gtttacgacg aactggagcg tctcgttagt ctggcgaagg aagaaatcag
    11641 ccagcttctg cctttagaag agcagctgga aaaactgatc gcgctgtttt acggcgactg
    11701 gggatttaaa gcctcacgcg gcgtttatcg tctttccgat gcattatggc tggaccaggt
    11761 gttaaagaat cgacagggca gtgcggtatc attaggtgcg gttttattat gggtcgcgaa
    11821 tcgtctcgat ttgccgctgc tgccggtgat tttccctacg cagctgatat tgcgcattga
    11881 atgtccggat ggcgaaattt ggctgattaa tccttttaac ggtgaatcgt taagcgaaca
    11941 tatgctggac gtatggttaa agggaaatat cagcccgtcg gcggaactgt tttatgaaga
    12001 ccttgatgaa gctgataaca ttgaggtaat ccgcaaattg ttggatacac tcaaagcctc
    12061 gttgatggaa gaaaatcaga tggagctggc gttacgcacc agcgaagctt tattacaatt
    12121 caacccggaa gatccctatg aaattcgcga tcgcgggttg atttatgcgc aactggattg
    12181 cgaacacgtt gcgttgaacg atttaagtta tttcgttgaa cagtgtccgg aagacccgat
    12241 cagcgaaatg atccgtgcgc aaataaataa catcgcgcat aaacatattg tgctgcatta
    12301 attaatcgac attttactca agattaaggc gatcctatga aacaaaaagt ggttagcatt
    12361 ggcgacatca acgtagcaaa tgacctgccg ttcgtactgt ttggtggtat gaacgtgctg
    12421 gaatctcgcg atctggcgat gcgcatttgc gagcactacg taaccgtgac ccagaaactg
    12481 ggtatccctt acgtgttcaa agcctctttt gacaaagcca accgctcctc catccactct
    12541 tatcgtggac cgggcctgga agaagggatg aaaatcttcc aggagttgaa gcagactttt
    12601 ggcgtgaaaa ttatcaccga cgttcacgaa ccaagtcagg cacagcccgt tgctgatgtc
    12661 gtggatgtga ttcagttgcc ggcgtttctt gctcgccaga ctgacctggt tgaagccatg
    12721 gcgaaaaccg gtgcggtaat taacgtcaag aaaccacagt ttgtcagccc gggacagatg
    12781 ggtaatatcg ttgataaatt caaagaaggc ggcaacgaaa aagtgattct ttgcgatcgc
    12841 ggtgctaact tcggctatga caacctggtt gtcgatatgc tgggcttcag cattatgaag
    12901 aaagtgtctg gtaactcgcc ggtgattttc gacgtgaccc acgcactgca atgccgcgat
    12961 ccgtttggcg cagcttccgg tggtcgtcgt gctcaggtgg ctgagctggc acgagccggt
    13021 atggcggtag gtctggcggg gctgtttatt gaagcgcatc cggatccgga acatgcgaaa
    13081 tgtgatggtc catccgcgct gccgctggct aaactggaac cgttcctcaa gcagatgaaa
    13141 gcgattgatg atctggtgaa aggtttcgaa gaactggata ccagcaagta atcttttttg
    13201 cttgaaaaat aaagtcttag cgttctgcgt taagactttt ttcatgggtg ccagatacaa
    13261 aaa
//
LOCUS       DAKISA010000115        13260 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.115, whole genome shotgun sequence.
ACCESSION   DAKISA010000115 DAKISA010000000
VERSION     DAKISA010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13260
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.115"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..781
                     /gene="umuC"
                     /locus_tag="OVW45_003506"
     CDS             <1..781
                     /gene="umuC"
                     /locus_tag="OVW45_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCV5527868.1"
                     /translation="VDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLA
                     DTDIRFIRKHFNVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQ
                     AICSYAARAAEKLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDII
                     NAATRSLDAIWQAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLN
                     AKEGRGTLYFAGQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="OVW45_003507"
     CDS             complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="OVW45_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCV5527869.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="OVW45_003508"
     CDS             complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="OVW45_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCV5527870.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     IGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            3265..3984
                     /gene="fadR"
                     /locus_tag="OVW45_003509"
     CDS             3265..3984
                     /gene="fadR"
                     /locus_tag="OVW45_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCV5527871.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(4036..5568)
                     /locus_tag="OVW45_003510"
     CDS             complement(4036..5568)
                     /locus_tag="OVW45_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCV5527872.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREMRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            5898..7196
                     /gene="dadA"
                     /locus_tag="OVW45_003511"
                     /pseudo
     CDS             5898..7196
                     /gene="dadA"
                     /locus_tag="OVW45_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
     gene            7206..8276
                     /gene="dadX"
                     /locus_tag="OVW45_003512"
     CDS             7206..8276
                     /gene="dadX"
                     /locus_tag="OVW45_003512"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCV5527873.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALVLRVPVVTV"
     gene            complement(8663..10399)
                     /locus_tag="OVW45_003513"
     CDS             complement(8663..10399)
                     /locus_tag="OVW45_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCV5527874.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="OVW45_003514"
     CDS             complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="OVW45_003514"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCV5527875.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRDERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            11508..12119
                     /gene="emtA"
                     /locus_tag="OVW45_003515"
     CDS             11508..12119
                     /gene="emtA"
                     /locus_tag="OVW45_003515"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCV5527876.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(12121..12855)
                     /locus_tag="OVW45_003516"
     CDS             complement(12121..12855)
                     /locus_tag="OVW45_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCV5527877.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            13056..>13260
                     /locus_tag="OVW45_003517"
     CDS             13056..>13260
                     /locus_tag="OVW45_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCV5527878.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAV"
BASE COUNT         3367 a         3372 c         3487 g         3034 t
ORIGIN      
        1 ggtggattta tcaaatctgg aacgccagcg taaattaatg tctgctctcc ccgtggatga
       61 cgtctggggg attggacggc ggatcagcaa aaaactggac gcgatgggga tcaaaaccgt
      121 tcttgatttg gcggatacag atatccggtt tatccgtaaa cattttaatg tcgtgctcga
      181 aagaacggtg cgtgaactgc gcggtgaacc ctgtttgcaa ctggaagaat ttgcaccgac
      241 aaagcaggaa attatctgtt cccgctcgtt tggtgaacgt atcacggatt atacgtcgat
      301 gcggcaggcc atttgtagtt acgctgcccg ggcggcggaa aaacttcgca gtgagcatca
      361 atattgtcgg tttatctcca catttattaa gacgtcacca tttgcgctca atgaacctta
      421 ttacggcaat agcgcgtcgg taaaactgct gacgcccact caggacagca gggatatcat
      481 taacgccgct acgcgatctc tggatgccat ctggcaagcg ggccatcgct accaaaaagc
      541 gggcgtgatg ctgggggatt tcttcagtca gggagtcgcg cagctcaatt tattcgatga
      601 caacgcaccg cgccccggga gtgagcaatt gatgacggta atggatacac tgaatgctaa
      661 agagggcaga ggaacactct attttgccgg gcaggggatc caacaacaat ggcagatgaa
      721 gcgagccatg ctttcaccac gttatacaac gcgaagttct gatttactgc gggtcaaata
      781 aatatagcgg caggaaaaaa gcgctcccgc aggagcgccg aatggattag cgaccgaaca
      841 gatcacgttt tttcgcttta aacggctggg aaatcatcac cagcaccgcg acaatcaggt
      901 aagcgataaa aataccgagc agccactgcg gcatttccag acctaaaaaa tcccactgac
      961 gctcggcgca atcgccagag gcgacaaaca cttgcggcac ccatttatcc agcggcagcc
     1021 attccgggaa acgaaccata aaatcacagg tggcaaacgg cgaaggatag agctgaagca
     1081 tggtgtgctc gtaagttaac tgcacaccgc ggaacgcact atacaaccag ataaccatcg
     1141 ctacataacg cagcggagtt ttcggggcga tcgcgccaat cagcgcagca cccagaacgc
     1201 cgaataacgc gcagcgttca taaatacaga gcacgcaagg tttcagtaac atcacatgct
     1261 ggaaccacag cgccgtcagt tccagtgcca gagcagtaaa cgccatcaac agccacgcgc
     1321 cccggccttg tgaacattgg ttcaaaaatc gcaacataat catttccctg caatatgcat
     1381 agagtgcgca gtttaaacca attcattcgc tgcgccacca ggaggcagca aaaataggac
     1441 gcaattggac aattatcggg cgaaaaggca aaccgggcat ttatatgccc ggtaagttgt
     1501 tatcaaagcg ttgctatcca gcccatttgc ataaaccatt cggttacagg ggcaagcgta
     1561 aactcgacgc agagcagtcc gacgagtgtc aggacgaggg tgtaaggcag ggccatccac
     1621 accatgcggc cataagagag gcgaatcaat ggcgcgagtg cagaggtcag caggaatagg
     1681 aacgcagcct gaccgttcgg cgtagcgaca gagggcagat tggtaccggt attaatggcg
     1741 accgccagca gctcgtattg cttcaacgtg atagcgccac tttccattgc cgctttcgct
     1801 tcgttgatat aaatcgtccc cacgaagacg ttatccgaaa tggatgacag cagaccgttg
     1861 aatatataga acagcgacag ctgagcatgt tccgatgcct gcaacacaaa ctgaataatc
     1921 ggcgaaaaca gttgttggtc gataatcacc gcgactaccg agaaaaacac cgtcaacagt
     1981 gcggtgaatg gcagagattc ggtgaaggct ttaccgatag catgctcatc ggtgacaccg
     2041 atcaatgatg tcgccagaat aatgactgac aaaccaatca aaccaacttc cgccagatgc
     2101 aacgccagcg cagtcaccaa ccagacgcca ataatcgcct ggacaatcag acggatttta
     2161 tcctgacggg tacgctggtg gcggctttga tcgtcaaact gctgcagcac ttcgcggact
     2221 ttctccggca gcgtttcacc gtagccaaac caacgcagct tctccaccag caggcaggtt
     2281 aacaggccac aaatcagaac cggaacggtc accggcgaca tgcgcaggaa gaaatcgcca
     2341 aaatgccagc cagccgcttt agcgatgatc aggttctgtg gttcgcccac catggtcatc
     2401 acgccgccta atgcggtgcc gacaccggca tgcatcatca ggctacgcag aaagccacgg
     2461 aactgttcca gaaccacttt gtaatgcttg tcgatatgac tatcgtcttg caggtcggta
     2521 tcttcagtac gggaagaggc tacgcggtga taaataccat aaaaaccgac tgcaacgctg
     2581 atcaccaccg ccacgacggt taaggcatcg aggaacgcgg agaggaacgc cgccgccacg
     2641 caaaaagaga gcgacagcag cattttggag cgaatgctta acagcaaacg ggtaaatatg
     2701 aacagcaata gctgtttcat aaaatagata cccgccacca taaacatcag taacagcaag
     2761 acttcaagat ttgccgccac ctcttcacgg acgtgttccg cgctggtcat gccgatgaat
     2821 accgcttcga tagccaacag accaccgggg agcagcgggt agcatttcag ggccatcgcc
     2881 agagtgaaaa taaattccgc taccagcaac cagcccgcga cgaaagggct gatgaggaaa
     2941 attaacgggt ttacgattaa gaaaatgatg agggcgagtt tgtaccagtc gggggactgg
     3001 cccaaaaaat tgcgccatag cgcgcggccc caggagatct ccatgatggt ttcccttacc
     3061 ttacaaataa tcaatgatgt ttttatgttt aaacgcaaag cttaacggtc aggcaggagt
     3121 gaggcaagtc ttgatagtca aggggaaaga gatgcggaaa atgaagcctt gatccctttt
     3181 tcttcttttt gtctgctatc agcgtagtta gccctctggt atgatgagtc caactttgtt
     3241 ttgctgtgtt atggaaatct cactatggtc attaaggcgc aaagcccggc gggtttcgcg
     3301 gaagagtaca ttattgaaag tatctggaat aaccgcttcc ctcccgggac tattttgccc
     3361 gcagaacgtg aactttcaga attaattggc gtaacgcgta ctacgttacg tgaagtgtta
     3421 cagcgtctgg cacgagatgg ctggttgacc attcaacatg gcaagccgac gaaggtgaat
     3481 aatttctggg aaacttccgg tttaaatatc cttgaaacac tggcgcgact ggatcacgaa
     3541 agtgtgccgc agcttattga taatttgctg tcggtgcgta ccaatatttc cactattttt
     3601 attcgcaccg cgtttcgtca gcatcccgat aaagcgcagg aagtgctggc taccgctaat
     3661 gaagtggccg atcacgccga tgcctttgcc gagctggatt acaacatatt ccgcggcctg
     3721 gcgtttgctt ccggcaaccc gatttacggt ctgatcctta acgggatgaa agggctgtat
     3781 acgcgcattg gtcgtcacta tttcgccaat ccggaagcgc gcagtctggc gctgggcttc
     3841 taccacaaac tgtcggcgtt gtgcagtgaa ggcgcgcacg atcaggtgta cgaaacagtg
     3901 cgtcgctatg ggcatgagag tggcgagatt tggcaccgga tgcagaaaaa tctgccgggt
     3961 gatttagcca ttcaggggcg ataatccctt ccgtttaaag agcaaacccc tcaaacgagg
     4021 ggttttttgt tgtttttaca gatttcccat tcttggcggg caacgttcca gcaactcgat
     4081 gctgccgtct tcgttttgct gttcgagcat cacatcaaat ccccacaggc gatgcacatg
     4141 cttcaggact tctttgcgcc cccgatccag cggtgcgcga ttatgtggaa tataacgcag
     4201 cgtcagcgaa cggtcgccgc gcaaatccac gttccagatc tgaatattcg gctccagatt
     4261 acttaagtta tattgcgacg ataaccggtt acgcatctcc cgataacctt cttcattatg
     4321 aatagcggaa atctccagat aattatgccg atcatcatcc agcacggtga agaagcggaa
     4381 atcacgcatc actttcggtg acagaaactg gctgataaaa ctttcatctt tgaaatcacg
     4441 catcgcgaaa tgcagcgttt ccagccagtc ggaaccggcg atatccggga accagtattt
     4501 gtcttcttcc gttggcgact gacaaatccg tttaatatcc tggaacatgg cgaacccgag
     4561 ggcatacggg ttgatgccgc tgtaccacgg gctgttatag gggggctgga agaccacatt
     4621 ggtgtggctg tgcaaaaact ccagcataaa acgttccgtt actttccctt catcatacag
     4681 atggttaagg atggtgtagt gccagaaggt cgcccagcct tcgttcatca cctgagtctg
     4741 tttttgcgga taaaaatact ggctcacctt acgcacaata cgcaggattt cacgctgcca
     4801 tgattccagc agtggcgcat ttttctccat aaaatagagc aggttttctt gtggttcgga
     4861 cggatagcgg cgcgcttcag caaccgtttt ctcttcctcg cgcttcggca gagtacgcca
     4921 gagcatattg acctgacttt gcagatactc ttcgcgactt ttctgccggg ctttctcttc
     4981 ttgcagcgag attttttgcg ggcgtttgta ccggtccacg ccgtagttca tcagcgcgtg
     5041 gcacgagtcc agaagccgtt ctacttcatc cacgccataa cgttcttcgc actcggtaat
     5101 atatttacgg gcaaaaatca gataatcgac aatcgaactg gcgtcggtcc aactgcggaa
     5161 taagtaattg tttttaaaga aagagttatg cccatagcag gcgtgtgcca tcaccagcgc
     5221 ttgcatggta atggtgttct cttccatcag gtaagcgata cacgggttag agttaatgac
     5281 gatttcatag gccagccctt gctgaccgtg cttatacagc cgttcagtct cgataaattt
     5341 tttaccgaat gaccagtgcg gatagttaat tggcatgccg acgctggagt aggcatccat
     5401 catctgttct gaggttatca cttcaatctg gtgcgggtag gtatccagcc ggtagagttt
     5461 cgccacccgg tctatctctg ccagataaac atccagcagg tcgaacgtcc agtcgggtcc
     5521 atcgctcaaa cgtgtggtgt ccttattcat agaatcgatc gtcgccatac gcgcacctca
     5581 ttgttgtcgg cgctctctgt gtggagcacc tcatttcaag catagaacac ctgttaaaaa
     5641 ccgcgtcgcc ggagaatttt tttctttgcg atttcttatt atcagagtgc cactaatccg
     5701 cttctgaacg gaattttatg ctggataaaa agggcgttca gcaggagata ctaaagacgc
     5761 catattgccg cagagtcagg gagatgtgag ccagctcacc ataaaaaagc cgtatgttga
     5821 ataatgtttt caactgagtt atcaagatgt aattagatta ttattctttt actgtatcta
     5881 ccgttatcgg agtggctatg cgagttgtca tactgggaag tggtgtggta ggcgttgcca
     5941 gcgcctggta cttaaatcag gcaggacatg aggtcaccgt cattgatcgg gagccggggg
     6001 ccgccctgga aaccagcgcc gcgaatgccg ggcaaatctc ccccggatat gctgcaccgt
     6061 gggcggcacc aggtgtgcct ttaaaagcga tcaaatggat gttccagcgc catgcgccgc
     6121 tggcggttcg tctcgacggt acgcagtttc agttgaaatg gatgtggtaa atgttacgta
     6181 actgcgacac cagccactac atggaaaaca aagggcggat ggtgcgtctg gcggaataca
     6241 gccgtgattg cctgaaagca ttgcgcgccg aaaccaatat tcagtatgaa gggcgtcagg
     6301 gtgggacgct gcaactgttc cgtaccgaac aacagtatga aaatgcgacc cgcgatatcg
     6361 ccgtgctgga agatgccggc gtaccgtatc agctgctgga atccagccgc ctggcggaag
     6421 tggagcccgc gctggcagaa gtagcgcaca aactgacggg tggtctgcaa ctgcccaatg
     6481 atgaaaccgg agactgccag ctgtttaccc agaatctggc gcggatggca gagcaggcgg
     6541 gggttaaatt ccgctttaat acgcccgttg accaactgct ttgcgacggc gagcaaatct
     6601 acggcgtgaa gtgtggcgat gaagtgatta aggccgatgc gtatgtgatg gcgtttggtt
     6661 cttactcgac ggcgatgctc aaaggcattg ttgatattcc ggtgtatccg ctgaaaggct
     6721 actcgctgac tattccaatt gcgcaggaag atggtgcgcc agtatccacc attcttgatg
     6781 aaacctacaa aatcgccatt acccgtttcg ataaccgcat tcgtgttggc ggaatggcgg
     6841 aaattgttgg ttttaacacc gagctgttgc aaccgcgtcg tgaaacgctg gaaatggtgg
     6901 ttcgcgatct ctatccacgc ggcggtcatg tcgagcaggc gactttctgg acggggttgc
     6961 gcccgatgac gccagacggt acgccggttg tcgggcgtac acgctttaaa aatctgtggc
     7021 tgaataccgg tcacggcacg ctcggctgga cgatggcttg cggttccggt cagttgttaa
     7081 gcgatctgct ctctggtcgc acgccagcga tcccatatga ggatctaagc gtagcgcgct
     7141 acagccgtgg atttacgcca tcacgtccgg gccatttaca tggcgcacac agctaaggaa
     7201 acgagatgac ccgtccgata caggccagcc tcgatctgca ggcattaaaa cagaatctgt
     7261 ccattgtccg ccaggccgcg ccgcacgcgc gcgtctggtc ggtggtaaaa gcgaacgctt
     7321 acgggcatgg tattgagcgt atctggagcg cgctcggggc caccgatggc tttgcattac
     7381 ttaacctgga agaggcaata acgttacgtg agcgcggctg gaaggggccg atcctgatgc
     7441 tggaaggatt tttccatgct caggatctgg agatttatga ccagcaccgc ctgaccacct
     7501 gcgtacacag caactggcag ctcaaagcac tgcaaaatgc gcggctaaaa gcaccgttgg
     7561 atatttatct taaagtgaac agtgggatga atcggttggg cttccagccc gatcgcgtgc
     7621 ttaccgtctg gcagcagttg cgggcaatgg cgaatgttgg cgagatgacc ctgatgtcgc
     7681 attttgccga agcggaacat cctgatggaa tttccagcgc gatggcgcgt attgagcagg
     7741 cggcggaagg gctggagtgt cggcgttcgt tgtccaattc ggcggcgact ctgtggcacc
     7801 cggaagcgca ttttgactgg gttcggcctg gcattatttt gtatggcgct tcgccgtccg
     7861 gtcagtggcg tgatatcgcc aataccggat tacgtccggt gatgacgcta agcagtgaga
     7921 ttattggtgt ccagacgcta aaagcgggtg agcgtgtggg ctacggcggt cgctatactg
     7981 cgcgcgatga acagcgaatc ggcattgtcg ccgcagggta cgccgacggt tatccgcgcc
     8041 acgcgcctac cggtacccct gttttagtgg acggcgtgcg caccatgacg gtggggaccg
     8101 tctcgatgga tatgctagcg gtcgatttaa cgccttgccc gcaggcgggt attggtacgc
     8161 cggttgagct gtggggcaag gagatcaaaa ttgatgatgt cgccgccgct gccggaacgg
     8221 tgggctatga gttgatgtgc gcgctggtgc tacgcgtccc ggttgtgacg gtgtaacttg
     8281 ttgtaagccg gatcggaggc aacgtcttct gggtgcaaaa aaaatcatcc atccggctgg
     8341 tcagcaactg tagttgttaa tgtgacagag ccattgccca tgatagtgtc cattaaaagg
     8401 atggacacta tttccccgga acctgaactc accgcacagg cgttctacat aaaacgctta
     8461 cgcttcattg ttgactcgaa ctcgacttca gataaatcac gctttaccct tgatggagcc
     8521 tgtacataga tttgtgtaat tgcctgattt tgatatgttc aattcaacat caaatgaagg
     8581 ttaaattatg gacgacaaac aattgcaggc tcaggctgcg ttcagcaaag catcgcaacc
     8641 ggcgatagat gcttcattaa atttaagatt cagcttcctc ttcagccacc cgtacgccaa
     8701 tcttcaacac ttcattatct tctttctcgg ccaccgtcca gatcatcccg gcaaactcta
     8761 cctggtcacc gacaaccggt gccgcgccta acaactgctg gacaatttca cccagcgttt
     8821 gctgcttatc acgatattct cgcccgtctt ccaggccata tatcagcgca acatcagcat
     8881 atttggcgct ggcttcgaga atgaagtcac caaagaagcg ttgatccagc gcgaccggtg
     8941 gcgactggct gaacagttta ccgagcgccg ggagatcgcg ttcccgacca attacacaca
     9001 acacatcgcc ttcgcgcagt cgggtgctgc cggtaggatg aagcaactgg ttatcacgaa
     9061 atagtgccgc aatacgcgtc tcttttggca tatgcagatc gcgcagtgcc gcacctacac
     9121 accatttatc ggcactcaat tgataaacaa actgctccca cggattttcc ggatgaatat
     9181 ccagaccaac gcgtgacacc ggacgtccga cgggcggaac caccactttg gcttttttcg
     9241 ccgcccacga gagtgatgtt ccctgcaaga gcagtgaaac cagaaccaca aagaaggcga
     9301 cattaaagaa cagacgtgca ttctccagcc ccgccatcat cgggaacacg gccaggatga
     9361 ttggcaccgc gccgcgtaat cctacccagc tgataaacac gcgctcacgc agattgaaac
     9421 cgcggaaggg gagcaatcca gcaaataccg aaaggggacg ggcgaagaat atcatccatg
     9481 ccgacaaaat gagcgccgga atggcaatag gcagcagatc gcttgggtta accaatagcc
     9541 ccagcaccag gaacatggcg atttgcgcca gccaggcgag gccgtcgaaa ttttgcagga
     9601 tgccgtagcg gttgcgaatc ggccgattac ccagcagaaa accgcacaga taaacagcca
     9661 gaataccgct gccttccagc gccgtggtta aagcaaaaat taaaataccg ccgcttaatg
     9721 ccagcaatgg atataatccg gcaggcaggg cgatgcgatt aatcatttgc agcagtaaat
     9781 aaccaccgcc aagtccgatg acaatcccga ggccaaattg ctgcagaata tcgaccacga
     9841 acatccaact gacactgctt tcatgttgct ggatcatcgc aatcagggta atcgtcagaa
     9901 agaccgccat tggatcatta ctgccggatt caatttccag cgtcgagcca acacgttcgt
     9961 taagcccctt accacccagc agagaaaaga ccgctgcagc atcggtagag ccgacgatag
    10021 cgccgattaa taagccttca atcaaatcaa gattaaacag ccacgccgcc atcatgccgg
    10081 ttaaaccaga ggtgataagc acgcccagcg tcgccagcga cagtgccggt cctaacgcca
    10141 cacgaaagga actggcctga gtgcgcatcc cgccgtcgag caaaataatc gccagtgcca
    10201 ggttacttac catgtaggcg aaggggtaat tatcaaacgg gataccgccg acgccatcga
    10261 ctcctgccag catgccgatc gccagaaaaa taaccagaat aggaatgcca agacgggaag
    10321 aaaatgaact aagtaatata ctgctggtga caaggatgga tcctaagatg aaaaggctaa
    10381 ttattgttgt ggcatccaac ggtcggttac tcctgattac gctgtctctt atataaaccc
    10441 taccatatta gcggcagaga cagcgtttta cttagccctg aagcgtgatt tttttacatt
    10501 ttaagaacag gatgaccgct aatcgtcaac tgagtgcctt cccgggtgtt attcagaatg
    10561 gcatgtgcgc ccaacggcag cgtaaccgtg cgttgttcat gaccaaaatc gaggccggta
    10621 atgagtggaa tcgacaggcg ggaacgcaaa aacgcgtaca ctgactccag gttgtaacct
    10681 gcgtcataat cattgggcgt gctgccgcta aagctaccga gaatgatcgc cttctgacgt
    10741 ggcaaaattc ccgcatgata gagctgcaac agcatacgtt cgtcccggaa agggtgctcg
    10801 ttaatatctt ccagcaccag aataccgttc tcaatttttg gcatccacgg tgtaccgatc
    10861 agtgaaatca gcatcgcaag attgcctccc cacaacgtgc cttcggcccg acatgtcggg
    10921 ccttcccctt gccattcaat ggtgaaggtt tcattgcgta acgccagcca gaaatggtgc
    10981 tcggtaaagg cgttcagttc atccgcgcca aagtttgcta ccagcatggg gccgctaaag
    11041 gtgatgacat tgccatgcgc cagaagaccg cactgaatgg cggtaaaatc gctatgcccg
    11101 caaatgagca acgggtcatg ttgttggcga gccaccagtg cctgccagtc aatatctgcc
    11161 agtaaacgac tggcaccgta accgccgcgt acagccagca cgatggtgtt gggggtggtc
    11221 agtctggcaa gggaattgag atcttccagg cgctccgttt ccgtacccgc aaaacgctca
    11281 caacgacggg caataacctc gacgttattg acctgatgcc cctcgtcggt aaggcgctgg
    11341 ataccacgca gcgcggcgtg ctgtttaatg cagtaacccg atggggcaat taagtgaaac
    11401 agagacatgg caattccttg ctgacaacag aaacgaaatg tatatcatgc cgcttaggtg
    11461 tgccgttgtc acctcaacgg cgattccagg ctataaggat agaagaagtg aaattgagat
    11521 ggtttgcctt tttgattgtg ttattagcgg gttgttcatc aaagcatgac tatacgaacc
    11581 cgccgtggaa cgcgaaagtt ccggtgcaac gtgcgatgca gtggatgcca ataagccaga
    11641 aagccggtgc agcctggggc gtcgatccac aattgatcac ggcgattatc gctatcgaat
    11701 cgggtggtaa tccgaatgcg gtgagtaaat cgaatgccat tggtttgatg cagttaaaag
    11761 cttcaacctc cggacgtgat gtttatcgcc gtatgggctg gagtggtgag ccgacgacca
    11821 gcgagctgaa aaatccggag cgtaatattt caatgggggc ggcttacctg aatattctgg
    11881 aaaccggccc gctggcaggc attgaagatc cgaaggtact gcaatatgcg ctggtggtgt
    11941 catacgctaa cggggcaggt gcgctgctac ggactttctc gtcagatcgg aaaaaggcga
    12001 tcagcaaaat caacgatttg gatgctgacg agttcctcga acacgtagcg cgaaatcacc
    12061 ctgcgccgca ggctccgcgc tatatctaca aacttgagca ggcactggac gcgatgtaaa
    12121 tcagtcgcgc actttgtccg ctttttcccg ggcttctcgc tcgagagaga aaataatccg
    12181 ctgtaattgc cgctccaccg tcgggctgac gttaagaaaa cggaagctca gacggggagt
    12241 ggtgatggtt tcattcttgc catcaatcac tttgcgctcg ctgatagaga ttaactgggc
    12301 gtcaaagtga aaaacacccc attgccccat gttgacttca atctgagcga agcgcatgcc
    12361 ttcttgtaat tcggcaggct ttgctgtttc cagtaatgcg cccatgccgc ctaacgacaa
    12421 atcatacagg cggaaacgta acgtactgtt atccgccagt ttggtctggc aaaaataagg
    12481 cggatggagt ggggcggaga tgcggaaata tcggcgtcgt tgtacaaacc ataaggtggg
    12541 aggcggtacg gtgataaatg ccggaagctg caagtattca ctctgctgta gttgttcaac
    12601 agtaaactcg actttcgcac cctgagtttc ggcggtaatg gtaatgtgct gtgcctttag
    12661 cacagcgatg ttgtcttcgg cttgactgcc gaaatccagc accagtttat ccggggttat
    12721 tgccagtatt ttgctgatca gctgcccgcc attccaactg agacgcaaag gaattgcggc
    12781 tttgtgcaaa tcgcgtaaca cgcccaggac ggctaacgga ttttgtttca ggaactgctc
    12841 atggtaatga ctcacgcgga aaaactcctg actgacaaac tgtctttggt ttatcggtca
    12901 cagttaacaa aacttaatac aaatgcgtga atatttttta catgttgttc ttaaatcagc
    12961 cgacatacgc ccagcatcga tcccgccctt gcctatactt agagcgttga cgtaagcatt
    13021 tcttgcgtcc gattcatcga aacgagggcc tgaacatggg aattattgct tggattattt
    13081 ttggcctgat agccggcatt atcgccaagc taatcatgcc ggggcgtgat ggtggtggat
    13141 ttttcctgac ctgtattctc gggatagtcg gtgcggtggt cggcggctgg ctggcgacca
    13201 tgtttggcat tggcggctcc atcagcggtt ttaatttgca cagcttcctg gtggcggtgg
//
LOCUS       DAKISA010000116        13197 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.116, whole genome shotgun sequence.
ACCESSION   DAKISA010000116 DAKISA010000000
VERSION     DAKISA010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13197
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.116"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            225..524
                     /locus_tag="OVW45_003518"
     CDS             225..524
                     /locus_tag="OVW45_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5527879.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     TLNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVFPVTNIVLFLILIFFKKR"
     gene            complement(571..1458)
                     /gene="rarD"
                     /locus_tag="OVW45_003519"
     CDS             complement(571..1458)
                     /gene="rarD"
                     /locus_tag="OVW45_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCV5527880.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1510..1977)
                     /locus_tag="OVW45_003520"
     CDS             complement(1510..1977)
                     /locus_tag="OVW45_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCV5527881.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELKRYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            2142..3011
                     /gene="pldA"
                     /locus_tag="OVW45_003521"
     CDS             2142..3011
                     /gene="pldA"
                     /locus_tag="OVW45_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCV5527882.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYCFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            3144..4973
                     /gene="recQ"
                     /locus_tag="OVW45_003522"
     CDS             3144..4973
                     /gene="recQ"
                     /locus_tag="OVW45_003522"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCV5527883.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            5037..5654
                     /gene="rhtC"
                     /locus_tag="OVW45_003523"
     CDS             5037..5654
                     /gene="rhtC"
                     /locus_tag="OVW45_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCV5527884.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLMVGGGLYLCWMGYQMLRGALKKEAVSAPA
                     PQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVETL
                     AWFIVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5716..6336)
                     /gene="rhtB"
                     /locus_tag="OVW45_003524"
     CDS             complement(5716..6336)
                     /gene="rhtB"
                     /locus_tag="OVW45_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCV5527885.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6447..7469
                     /gene="pldB"
                     /locus_tag="OVW45_003525"
     CDS             6447..7469
                     /gene="pldB"
                     /locus_tag="OVW45_003525"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCV5527886.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7477..8277
                     /gene="yigL"
                     /locus_tag="OVW45_003526"
     CDS             7477..8277
                     /gene="yigL"
                     /locus_tag="OVW45_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCV5527887.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNLDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            8353..9252
                     /gene="bioP"
                     /locus_tag="OVW45_003527"
     CDS             8353..9252
                     /gene="bioP"
                     /locus_tag="OVW45_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCV5527888.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(9140..10093)
                     /gene="metR"
                     /locus_tag="OVW45_003528"
     CDS             complement(9140..10093)
                     /gene="metR"
                     /locus_tag="OVW45_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCV5527889.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFEHQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10329..12590
                     /gene="metE"
                     /locus_tag="OVW45_003529"
     CDS             10329..12590
                     /gene="metE"
                     /locus_tag="OVW45_003529"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCV5527890.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12875..13120)
                     /locus_tag="OVW45_003530"
     CDS             complement(12875..13120)
                     /locus_tag="OVW45_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCV5527891.1"
                     /translation="MITETEINVLKIMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
BASE COUNT         3132 a         3261 c         3502 g         3302 t
ORIGIN      
        1 atttgaacat ttttacattg ttttatatat ataattgttt tgaatatata ttacctgaat
       61 tatgtggttt gcatatatat atgcaatata gcagtctttg cattgataac tatacagtag
      121 taatttagag tgtgtaagtt tttattgatg gggtatattt taattaaata taattgaaat
      181 gtatttttat tctataaaca atatcttggg tgaatggatt ctatatggga aatggtaact
      241 tttggcagtt gttcttgggt aaagaacctt acagcaaaaa atctgatttt gttaaggcat
      301 ggttattgtg gtgggctatt accatccttg ttctatttat tttaagcctg actacgacat
      361 taaatttaag aagtataggt atagggttaa gttttgggtt ttgtcaaatc cttggtagat
      421 atacgatgtc tctaggatgg agtaagtact ggggagtttt gggggtattt cctgtaacaa
      481 atattgttct ttttcttatc ttaatttttt tcaaaaaaag ataaataagt cagtgggtag
      541 gctctttaaa cggcctaccc atgttaattc ttaacttttt ctacgctgcg tataaatcgc
      601 atccatcaca aaaattgcca gcgccaccca aataaaggcg aaagtcacca tcttatcggc
      661 acccggtttt tcaccataaa acgtcacagc cagcaggaac atcagcgtcg ggccaatgta
      721 ctggaaaaag cctaacgttg agagacgcaa gcgcgtggca gcggcggtaa aacacaacag
      781 cggtacggta gtgacaatac cggcggcgat cagcagtaaa ttcagcgaca tcgggttttg
      841 ccccatatgg ctggttgagc tgtcggcaat agcaaacagg taaattgccg ccacgggcag
      901 cagccacatg gtttcgatta acatgccggt ttgcgcttca acggcaatct tcttgcgtac
      961 cagaccgtag aaggcaaaac taaatgccag tcccagcgcg ataataggta gcgaaccaaa
     1021 agtccacagc tggactaaca cgccacatat cgccagaatc accgccagcc attgcatccg
     1081 gcggaatcgc tcgccgagga aaatcatccc cagcacaatg ttcaccagcg ggttaataaa
     1141 gtaaccaagg ctcgcttcca gcatatggtg attgttcacc gcccagataa acagtagcca
     1201 gttgccacca atcagcacgg cagagactgc cagcataaaa attttctgtg gcgtctgaat
     1261 cagcgttttt aaataggacc actggcggca aatgctcatc agcaccacca taaagaaaaa
     1321 cgaccagatc acgcgatgcg tcaggatttc atcggcgggc acgtagtaaa tcaacttgaa
     1381 gtacgctggc gctatacccc aaataaaata agcggcaaga gcgagtaata cgccctgccg
     1441 cgtttgtttt gcatccatcg ggaatactca tttttaattg gtaacagcag tttacctgct
     1501 tttatgtctt caacctacca tataggtggc ggtggcactg gcaatataaa gctgttcttc
     1561 attgtgtaat tcaacgcggg cgacggcgac tttattgcct gcacgcaaca ggctactggt
     1621 agcagtaaaa cgctcgcccc tgcctgggcg cagataatca acgcgaagat caatggtccc
     1681 catccgcgat agccgctggc gtagttcatc ttcactgatg gtttcgtggc gggttaaggt
     1741 acttcccacg cacaccagac cggcggcgac atccagcgcc gacgcaatga ccccgccgtg
     1801 caaaatgctt tgcgcccagt tgcccaccat cattggctga tttttaaagg ccagctgtgc
     1861 gaactctttt tcgtaacgct tcagttccat ccccaatgcg cggttaaatg gcatgtgata
     1921 aacaaacatc tcacccacta atttcagggc ttgttcagcg gtcagtacgg cagacatatc
     1981 atccttacac ttcattggtt aatgaaatgt tgattttatg cttctttgtt gttggtttct
     2041 actttaggaa gggataacta acggctatgg agttaagtat gtaaaatagc ccgcagaaaa
     2101 atattcacct tatcaataat tcgttacgga gaacacgacc gatgcggact ctgcagggct
     2161 ggttgttgcc ggtgtttatg ttgcctatgg cagtatatgc acaagaggca acggtgaaag
     2221 aggtgcatga cgcgccagcg gtgcgtggca gtattatcgc caatatgctg caggagcatg
     2281 acaatccgtt cacgctctat ccttatgaca ccaactacct catttacacc caaaccagcg
     2341 atctgaataa agaagcgatt gccagttacg actgggcgga aaatgcgcgt aaggatgaag
     2401 taaagtttca gttgagcctg gcatttccgc tgtggcgtgg gattttaggc ccgaactcgg
     2461 tgttgggtgc gtcttatacg caaaaatcct ggtggcaact gtccaatagc gaagagtctt
     2521 caccgtttcg tgaaaccaac tacgaaccgc aattgttcct cggttttgcc accgattact
     2581 gttttgcagg ttggacgctg cgcgatgtgg agatggggta taaccacgac tctaacgggc
     2641 gttccgaccc gacctcccgc agctggaacc gcctttatac tcgcctgatg gcagaaaacg
     2701 gtaactggct ggtagaagtg aagccgtggt atgtggtggg taatactgac gataacccgg
     2761 atatcaccaa atatatgggt tactaccagc ttaaaatcgg ctatcacctc ggtgatgcgg
     2821 tgttgagtgc gaaaggacag tacaactgga ataccggtta cggcggcgcg gagttaggct
     2881 taagttaccc gatcaccaaa catgtgcgcc tttatactca ggtttacagc ggctatggcg
     2941 aatcgctcat cgactataac ttcaaccaga cccgtgtcgg tgtaggagtt atgctaaacg
     3001 acttgttttg atgaacggtt gagtggttgg caaatctgga acccagcatc caggattacc
     3061 ctctcagaga ctaaaagcat tgcagtttct cgcgcaggcg ctgaaaatag cgcctgtttt
     3121 tatttcaggc aatcggggtg aatgtggcgc aggcggaagt gttgaatctg gagtccggag
     3181 ctaaacaggt tttacaagaa acctttggct accaacagtt tcgccccggc caggaagaaa
     3241 ttatcgacac tgtgctttcc ggtcgcgatt gcctggtcgt catgcccacc ggtggcggaa
     3301 aatccctttg ctatcaaatt cctgccttat tgctaaacgg ccttaccgtg gttgtttcac
     3361 cgctgatttc gttgatgaaa gatcaggtgg atcaactgca agccaacggc gtggcggcgg
     3421 cgtgccttaa ctcgacgcaa acccgcgagc agcaacttga agtgatgaca ggctgccgca
     3481 ccgggcaaat tcgtctgctt tatatcgccc cggaacgcct gatgctggat aactttcttg
     3541 agcatctggc gcactggaat ccggtgttat tagccgttga tgaagcgcac tgtatctccc
     3601 aatggggcca cgatttccgc ccggaatatg ccgcgcttgg tcagttacgc cagcgtttcc
     3661 cgaccctgcc gtttatggcg ctgactgcca cagccgacga caccacgcgc caggatatcg
     3721 tgcgcttgct ggggctgaac gatccgctga ttcaaatcag cagttttgac cgtccgaata
     3781 ttcgctacat gctgatggag aaattcaaac cgctcgatca gttgatgcgc tacgtgcagg
     3841 aacagcgcgg taagtccggc attatctact gcaacagccg ggcgaaagta gaagacaccg
     3901 ctgcgcgcct gcaaagcaag ggtattagcg cggcggccta tcatgccggg ctggaaaata
     3961 atgtccgcgc cgacgtgcag gagaaattcc agcgcgatga cctgcaaatt gtggtggcga
     4021 cggtggcgtt cggcatgggc atcaataaac caaacgttcg cttcgtggtc cactttgata
     4081 ttccgcgcaa tatcgaatcc tattatcagg aaaccggtcg cgccgggcgt gatggcctgc
     4141 ctgcggaagc gatgctgttt tacgatccgg ctgatatggc gtggctgcgc cgttgtctgg
     4201 aagagaagcc gcaggggcag ttgcaggata tcgagcgcca caaactcaat gcgatgggcg
     4261 cgtttgccga agcgcaaact tgccgtcgtc tggtattgct gaactatttt ggcgaagggc
     4321 gtcaggagcc gtgcgggaac tgcgatatct gcctcgatcc gccgaaacag tacgacggtt
     4381 caaccgatgc tcagattgcc ctttccacca ttggtcgtgt gaatcagcgg tttgggatgg
     4441 gttatgtggt ggaagtgatt cgtggtgcta ataaccagcg tatccgcgac tatggtcatg
     4501 acaaactgaa agtctatggc atgggccgtg ataaaagcca tgaacattgg gtgagcgtga
     4561 tccgccagct gattcacctc ggcctggtga cgcaaaatat tgcccagcat tctgccctac
     4621 aactgacaga ggccgcgcgc ccggtgctgc gcggcgaatc ctctttgcaa cttgccgtgc
     4681 cgcgtatcgt ggcgctcaaa ccgaaagcga tgcagaaatc gttcggcggc aactatgatc
     4741 gcaaactgtt cgccaaatta cgcaaactgc gtaaatcgat tgccgatgaa agtaatgtcc
     4801 cgccgtacgt ggtgtttaac gacgcaacct tgattgagat ggctgaacag atgccgatca
     4861 ccgccagcga aatgctcagc gttaacggcg ttgggatgcg caagctggaa cgctttggta
     4921 aaccgtttat ggcgctgatt cgtgcgcatg tcgatggcga cgacgaagag tagtcagcag
     4981 cataaaaaag tgccaggatg aagactccgt aaacgtttcc cccgcgagtc aaatgtatgt
     5041 tgatgttatt tctcaccgtc gccatggtgc acattgtggc gcttatgagc cccggtcccg
     5101 atttcttttt tgtctctcag accgctgtca gtcgttcccg taaagaagcg atgatgggcg
     5161 tgctgggcat tacctgcggt gtaatggttt gggctgggat tgcgttgctt ggcctgcatt
     5221 tgattatcga aaaaatggcc tggctgcata cgcttatggt tggcggtggc ctgtatctct
     5281 gctggatggg ttaccagatg ctgcgcggcg cgctgaaaaa agaggcggtt tctgcacctg
     5341 cgccacaggt cgagctggcg aaaagtgggc gcagtttcct gaaaggttta ctgaccaatc
     5401 tcgctaatcc gaaagcgatt atctactttg gctcggtgtt ctcattgttt gtcggtgata
     5461 acgttggcac taccgcgcgc tggggcattt ttgcgctgat cattgtcgaa acgctggcgt
     5521 ggtttatcgt cgttgccagc ctgtttgccc tgccgcaaat gcgccgtggt tatcaacgtc
     5581 tggcgaagtg gattgatggt tttgccgggg cgttatttgc cggatttggc attcatttga
     5641 ttatttcgcg gtgatgccag acgcgtcttc agagtaagtc ggataaggcg tttacgccgc
     5701 atccgacatt atttttcacg catgcctcgc cgatgctaac agcgctccca ccagcataaa
     5761 caacgagccg aaaatcttat tcagcgcctt catctgcttt ggtcctttaa tccatagagc
     5821 aatccgttga gcaagggtgg cgtaaccgat catcacaata atatcgacca caatagtggt
     5881 gacgccgagc acgatatact gcatcagttg cggctgttgc ggcatgatga attgcggaaa
     5941 tagcgccgcc agaaacacaa tacttttggg attggtgaga ttcacaaaaa ctgcgcgctg
     6001 gaacaaatgt cgacgcgatt gagtagaggc cagcgattta aggtcaattg caccagcggc
     6061 gcgccactgc tggattccca gccaaatcaa gtaagccgcg cctgcccact tcaacacttc
     6121 aaacgcaatc actgagcggg aaaatagcgt ccccaacccc acgccaacca gcacaatatg
     6181 aatcgccagt ccggtctgaa gcccagcaat agacgccacc gcgccgcgat aaccgtggtt
     6241 gagcgaggtg gtcatagtgt tgattgcacc agagcctggc gacaggctta aaatgatcga
     6301 tgtcagcagg taggcaaacc accattctaa ggtcatgatg aactcccggt gtgtctattt
     6361 ttgtgccaca atacgctact gtcgcagcgt tgtgtcaggc acgctaaaaa aaacgatttt
     6421 acgtggttta agaggcagat tacccgatgt ttcagcagca aaaagactgg gaaacaagag
     6481 aaaacgcgtt tgctgctttt accatgggac cgctgactga tttctggcgt cagcgtgatg
     6541 aagcagagtt tactggtgtg gatgacattc cggtgcgctt tgtccgtttt cgcgcacagc
     6601 accatgaccg ggtggtagtc atctgcccgg ggcgtattga gagctacgta aaatatgcgg
     6661 aactggccta tgacctgttc catttggggt ttgatgtctt aatcatcgac catcgcgggc
     6721 agggacgttc cggtcgcctg ttagccgatc cgcatctcgg gcatgttaat cgctttaatg
     6781 attatgttga tgatctggcg gcattctggc agcaggaggt tcagcccggt ccgtggcgta
     6841 aacgctatat actggcacat tcgatgggcg gtgcgatctc cacattattt ctgcaacgcc
     6901 atccaggtgt atgtgacgcc attgcgctaa ctgcgccaat gtttgggatc gtgattcgta
     6961 tgccgtcatt tatggcacgg cagatcctca actgggccga agcgcatcca cgtttccgtg
     7021 atggctatgc aataggcacc gggcgctggc gcgcgttgcc gtttgctatc aacgtactga
     7081 cccacagcag acagcgatat cgacgtaact tacgcttcta tgctgatgac ccaacgattc
     7141 gcgtcggtgg gccgacctac cattgggtac gcgaaagtat tctggctggc gaacaggtgt
     7201 tagccggtgc gggtgatgac gccacgccaa cgcttctctt gcaggctgaa gaggaacgcg
     7261 tggtggataa ccgcatgcat gaccgttttt gtgaactccg caccgccgcg ggccatcctg
     7321 tcgaaggagg acgaccgttg gtaattaaag gtgcttacca tgagatcctt tttgaaaagg
     7381 acgcaatgcg ctcagtcgcg ctccacgcca ttgttgattt tttcaacagg cataactcac
     7441 ccagcggaaa ccgctctaca gaggtttaaa tttcttatgt accaggttgt tgcgtctgat
     7501 ttagatggca cgttactttc tcccgaccat acgttatccc cttacgccaa agaaaccctg
     7561 aagctgctca ccgcgcgcgg catcaacttt gtgtttgcga ccggtcgtca ccacgttgat
     7621 gtggggcaaa ttcgcgataa tctggagatc aagtcgtaca tgatcacctc caacggcgcg
     7681 cgcgttcacg atctggatgg caatctgatt tttgctcata acctggatcg cgacattgcc
     7741 agcgatctgt ttggcgtagt caacgacaat ctggacatca ttactaacgt ttatcgcgac
     7801 gacgaatggt ttatgaatcg ccatcgcccg gaagagatgc gcttttttaa agaagcggtg
     7861 ttcaaatatg cgctgtatga gcctggatta ctggagccgg aaggcgtcag caaagtgttc
     7921 ttcacctgcg attcccatga acaactgctg ccgctggagc aggcgattaa cgctcgttgg
     7981 ggcgatcgcg tcaacgttag tttctctacc ttaacctgtc tggaagtgat ggcgggcggc
     8041 gtttcaaaag gccatgcgct ggaagcggtg gcgaagaaac tgggctacag cctgaaggat
     8101 tgtattgcgt ttggtgacgg gatgaacgat gccgaaatgc tgtcgatggc ggggaaaggc
     8161 tgcattatgg gcagtgcgca ccagcgtctg aaagaccttc atcccgagct ggaagtgatt
     8221 ggtactaatg ccgacgacgc ggtgccgcat tatctgcgta aactctattt atcgtaatcg
     8281 ttctttattt ggtcagttgt caacctgata cttcgctaca atggatactc gttaatcaaa
     8341 gagttttcca ttgtggcgct acttatcatc accacgattc tgtgggcctt ctcctttagc
     8401 ttttatggcg agtaccttgc ggggcacgtc gatagctatt ttgcggtgct ggtgcgcgtt
     8461 ggcctggcgg cactcgtttt tctgccgttt ctgcgtaccc gtggcaatag cctgaaaacg
     8521 gtcggcctgt atatgctggt gggcgcgatg cagctcggcg tgatgtatat gctgagtttc
     8581 cgcgcttatc tctacctgac ggtctccgaa ttgttgctgt tcaccgtact gacgccgctc
     8641 tacatcacgc tgatttatga catcatgagt aagcgccgtc tgcgctgggg ctatgctttt
     8701 agcgccttgc tggcggtgat tggtgccggg attattcgct atgatcaggt taccgaccat
     8761 ttctggactg gcttgttgct ggtgcaactc tccaatatca cttttgccat cggcatggtg
     8821 ggttacaaac gcctgatgga aactcgcccg atgccgcagc ataacgcctt tgcgtggttc
     8881 tatcttggcg cgttcctggt ggcggtaatt gcatggttct tgctgggaaa tgcgcagaaa
     8941 atgccgcaaa ccacgttgca atggggcatt ctggtgtttc ttggcgtggt ggcttccggg
     9001 attggctact ttatgtggaa ctacggcgcg acgcaggtgg acgccggaac gctgggcatt
     9061 atgaataata tgcacgttcc ggcagggctg ctggtaaacc tggctatctg gcaccaacag
     9121 ccgcactggc caacgttcat tacaggcgcg ctggtgatcc tggcctcact gtgggtgcat
     9181 cgtaagtggg tcgctccgcg ctcttcacaa acggcagatg atcgcaggcg tgattgcgcg
     9241 ctgagcgaat aaatgcttct gtaatcggct gacgctgctc gccatcgcgc acggcggcgt
     9301 acagtcggct ccacaagcct tcgcccaggg ttttggtcac caccagaccc tggtgctcaa
     9361 aactctctac tacccaatgc ggtagcgcgg caatacccat ccgcgcggca accatctgaa
     9421 tcaacaataa ggtgttatcg acgcttttca gtgacgggct gacgcctgcc ggctgaagaa
     9481 aatgccgcca gacatccagt cgactacgct gcaccggata aatcaacagc gtctcgctgg
     9541 cgagatcttc cggtgtaatt cgcgttttcg ccgccagtgg atggtcaggt gctaacacca
     9601 gacgcacttc atagtcgaac atcggcgaat aatgcaggcc actgcgcggc aggatatcgg
     9661 aggtcatcac cagatccagc tcgccctgtt gcaacgcagg ctgcgggtca aacgtcacgc
     9721 ccgatttaaa atccatctct acctgcggcc agttcttatg gaaattttct aacgcgggcg
     9781 tcagccactg aatacagcta tggcactcaa tggcaatgcg cagacgcgtc tgctgcggtt
     9841 cattgcaggc ttgcagtgcc tggctgattt gcggcagcac ctggtttgcc agttgcaaca
     9901 ggatttctcc ctgcggtgta aagcgtagcg gctggctctt acgcacaaat agccggaagc
     9961 caaggcgttg ttccagatcg ctaaactggt gagacagggc ggattgcgtc tgatgcaacg
    10021 tcgccgcagc ggctgcgaga gagccgcagt tccgcaacgc ttgtagcgtt ttcaggtgtt
    10081 ttacttcgat catgaaagtc cttcacttcg gcatgaataa tttgcgcttg aggaatatac
    10141 agtaaccgcc aattatggat gtgtaaacat ctggacggct aaaatccttc gtcttttaaa
    10201 tttatggtgc gttggctgcg tttctccacc ccggtcactt acttcagtaa gctcccgggg
    10261 atgaataaac ttgccgcctt ccctaaattc aaaatccata ggatttacat ataattagag
    10321 gaagaaaaat gacaatattg aatcacaccc tcggtttccc tcgcgttggc ctgcgtcgcg
    10381 agctgaaaaa agcgcaagaa agttattggg cggggaactc cacgcgtgaa gaactgctga
    10441 cagtagggcg tgaactgcgt gctcgtcact gggatcaaca aaagcaagcg ggtatcgacc
    10501 tgctgccggt gggcgatttt gcctggtacg atcatgtact gaccaccagt ttgctgctgg
    10561 gtaatgttcc gccacgtcat cagaacaaag acggttcggt agatatcgac accctgttcc
    10621 gtattggtcg tggacgtgcg ccgactggcg aacctgcggc ggcagcggaa atgaccaaat
    10681 ggtttaacac caactatcac tacatggtgc cggagttcgt taaaggccaa cagttcaaac
    10741 tgacctggac gcagctgctg gaggaagtgg acgaggcgct ggcgctgggc cacaacgtta
    10801 agcctgtgct gctggggccg gttacttacc tgtggctggg gaaagtgaaa ggtgaacagt
    10861 ttgaccgcct tagcctgctg aacgacattc tgccggttta tcagcaagtg ctggcagaac
    10921 tggcgaaacg cggcatcgag tgggtacaga ttgatgaacc cgcgctggta ctggaactac
    10981 cacaggcgtg gctggacgca tacaaacccg cttacgacgc gctccaggga caggtgaaac
    11041 tgctgctgac cacctatttt gaaggcgtaa cgccaaatct cgacacgatt actgcgctgc
    11101 ctgttcaggg tctgcatgtt gatctcgtac atggtaaaga tgacgttgct gaactgcaca
    11161 agcgcctgcc ttctgactgg ttgctgtctg cgggtctgat caatggtcgt aacgtctggc
    11221 gcgccgatct taccgagaaa tatgcgcaaa ttaaggacat tgtcggcaaa cgtgatttgt
    11281 gggtggcatc ttcctgctcg ttgctgcaca gccccatcga cctgagcgtg gaaacgcgtc
    11341 ttgatgcaga agtgaaaagc tggtttgcct tcgccctaca aaaatgccat gaactagcac
    11401 tgctgcgcga tgcgctgaac agtggtgaca cggcagctct ggcagagtgg agcgccccaa
    11461 ttcaggcgcg tcgtcactct actcgtgtac ataatccggc ggtagaaaag cgtctggcgg
    11521 cgatcaccgc ccaggacagc cagcgtgcga atgtctatga agtgcgtgct gaagcccagc
    11581 gtgcacgttt taaactgcca gcgtggccga ccaccacgat tggttccttc ccgcaaacca
    11641 cggaaattcg taccctgcgt ctggatttca aaaagggcaa tctcgacgcc aataactacc
    11701 gcacgggcat tgcggaacat atcaggcagg ccattgttga acaggaacgt ttgggactgg
    11761 atgtgctggt acatggcgag gccgagcgta atgacatggt ggaatacttt ggcgagcacc
    11821 tcgacgggtt tgtctttacg caaaacggtt gggtacagag ctacggttcc cgctgcgtga
    11881 agccaccgat tgttattggt gacgttagtc gcccggcacc gattaccgtg gagtgggcga
    11941 agtatgcgca atctctgact gataaaccgg tgaaagggat gctgactggc ccggtgacta
    12001 ttctctgctg gtcgttcccg cgtgaagatg tcagccgtga aaccatcgcc aaacagattg
    12061 cgctggcgct gcgtgatgaa gtggccgatc tggaagccgc tggaattggc atcatccaga
    12121 ttgacgaacc ggctctgcgc gaaggtttac cgctgcgtcg tagcgactgg gatgcgtatc
    12181 tccagtgggg cgtagaggcc ttccgtatca acgccgccgt ggcgaaagat gacacacaaa
    12241 tccacactca catgtgttat tgcgagttca acgacatcat ggattcgatt gcggcactgg
    12301 acgcagacgt catcaccatc gaaacctcgc gttctgacat ggagttgctg gagtcgttcg
    12361 aagagtttga ttatccgaat gaaatcggtc caggcgtcta tgacattcac tcgccaaacg
    12421 taccgagcgt ggaatggatt gaagccttgc tgaagaaagc ggcaaaacgc attccggcag
    12481 agcgcctgtg ggttaacccg gactgtggcc tgaaaacgcg cggctggcca gaaacccgcg
    12541 cggcactggc gaacatggtg caggcggcgc agaatttgcg tcggggatga tgaatattaa
    12601 cgagtcactg taaattgcag tacgatgaaa gaggcaaaac tgcctgatac taggcctaca
    12661 taatttatgc aatttattga atttttgggt tttgttggcc ggacaaggcg ttcacgccgc
    12721 atccggcatg aacaacgcgc actttgccaa taatctgatg gcttccatca ccggaagcca
    12781 ttttgctatc tgcgcccgtt cagatacaga aataaccgaa tcatttttac gcaaaatata
    12841 atcctgagcg atttgttcag taatcaacgc tacgttattt attctcactt ttcacgaaat
    12901 tgtatccctt ttcagtaacg tggtagtgag gcatagactt atgtccgcta tcctcaataa
    12961 taaccaaacc ctcgttagct aatttgttaa caatgttaac aacattaccg aatcctggaa
    13021 tttgccttat ggatagtgtg cgatcgagat tcatccagtt ccagtttata tctttctcag
    13081 ccataatttt cagtacgtta atttcagttt cagttatcat agtcaagtgc atcctggtgg
    13141 tataagtcat taagttctcc cttaagttta taatcttcta gagtagcagt atgcgta
//
LOCUS       DAKISA010000117        12970 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.117, whole genome shotgun sequence.
ACCESSION   DAKISA010000117 DAKISA010000000
VERSION     DAKISA010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12970
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.117"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            519..2771
                     /locus_tag="OVW45_003531"
     CDS             519..2771
                     /locus_tag="OVW45_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5527892.1"
                     /translation="MFSIKPGPRNLPIDNPTLLSWNITDGDLNSKLNTLEYLNCITNI
                     INSCGVYPQGLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQRSITAP
                     DRIGEGKTWVYQRDTMIERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRENMSEFARCHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIWGSGKNDLDYPSADIRAKI
                     SKDIEGGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWEN
                     LHALKEKRLNGIPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLKNYNERLHNTNLFNPKYHKKAVMNYMEYLVTCQLDEQQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPGKVFTLRGGTLNINIPGRSALLLGK
                     TGEPPNYLYL"
     gene            3107..4084
                     /gene="csiD"
                     /locus_tag="OVW45_003532"
     CDS             3107..4084
                     /gene="csiD"
                     /locus_tag="OVW45_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCV5527893.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            4104..5372
                     /gene="lhgO"
                     /locus_tag="OVW45_003533"
     CDS             4104..5372
                     /gene="lhgO"
                     /locus_tag="OVW45_003533"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCV5527894.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            5395..6843
                     /gene="gabD"
                     /locus_tag="OVW45_003534"
     CDS             5395..6843
                     /gene="gabD"
                     /locus_tag="OVW45_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HCV5527895.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGGIIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            6857..8137
                     /gene="gabT"
                     /locus_tag="OVW45_003535"
     CDS             6857..8137
                     /gene="gabT"
                     /locus_tag="OVW45_003535"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HCV5527896.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEHENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            8448..9848
                     /gene="gabP"
                     /locus_tag="OVW45_003536"
     CDS             8448..9848
                     /gene="gabP"
                     /locus_tag="OVW45_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCV5527897.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            9869..10531
                     /gene="csiR"
                     /locus_tag="OVW45_003537"
     CDS             9869..10531
                     /gene="csiR"
                     /locus_tag="OVW45_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCV5527898.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(10532..10981)
                     /gene="kbp"
                     /locus_tag="OVW45_003538"
     CDS             complement(10532..10981)
                     /gene="kbp"
                     /locus_tag="OVW45_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCV5527899.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(11065..11223)
                     /locus_tag="OVW45_003539"
     CDS             complement(11065..11223)
                     /locus_tag="OVW45_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCV5527900.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            11406..11705
                     /locus_tag="OVW45_003540"
     CDS             11406..11705
                     /locus_tag="OVW45_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCV5527901.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            11715..12239
                     /gene="ygaP"
                     /locus_tag="OVW45_003541"
     CDS             11715..12239
                     /gene="ygaP"
                     /locus_tag="OVW45_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCV5527902.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(12286..12690)
                     /gene="stpA"
                     /locus_tag="OVW45_003542"
     CDS             complement(12286..12690)
                     /gene="stpA"
                     /locus_tag="OVW45_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCV5527903.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         3334 a         3136 c         3225 g         3275 t
ORIGIN      
        1 atgatgatca tatgatgtaa ataacagatt tttatgcgtt ctcattcatc cttgctcgtc
       61 attacacgcg acataaaacc cgacaccgct tccattcgca aagtcgatac tcgcagtcaa
      121 caagcaaatg ttaataatta gcactatcta tagttatcat cgattcaatg atagtttgta
      181 atgattttgt atctaataat ataactttat tacattagct gaagagtttt cgcatcatta
      241 tgatatctgt tacttttcac tccataaaaa taaacttcat atagcaatat attctttcat
      301 agatcttatt ctgctaatca ttagtttcgt atgagcgatt tttgacagtt gcctctccag
      361 accacatcga taattaataa aacagattta agcattatcc ttttccatat aaatattgga
      421 taaaagtagg acatctgttt gcaattactt tcacaacaat taaacatttt tatgtttccg
      481 tatacatcat attactctac cattagagga actttattat gttttctata aaaccaggac
      541 ccagaaattt acctatcgac aaccccacat tgttatcatg gaacattact gacggggatc
      601 taaattccaa attaaataca ttagaatatc taaactgtat aacaaatatt attaattctt
      661 gtggagttta ccctcaagga ttaaaagaca gagaaattat atcaactttt cacgcagaaa
      721 aagttattaa tgatctgtta aaaaacgatt ataaaatttc cctttctcca gatacaactt
      781 atcgagagtt gaataaagca gcacagcgta gcattacagc gccagacagg ataggagaag
      841 gaaaaacatg ggtttatcaa cgagatacaa tgattgaaag aggtgataac agcggtgttc
      901 atcagtatgg tccagctgaa catttcactc acattatatc tgacaaaccg tccccaaaag
      961 ataagtatgt tgcatatgct attaacattc ctgactatga gctggcagcc gatgtatata
     1021 atattaacgt tacgtcacct tccggacagc aagaaacatt taaaatacta atcaatccag
     1081 aacatctacg gcagacactt gagcgtaaat ctcttactgc tgttcagaaa tcacaatgtg
     1141 aaatcatcac ccctaaaaaa cccggcgaag cgattcttca tgcttttaat gccacctacc
     1201 agcaaatcag ggaaaatatg tctgagtttg cacgttgcca ttatgggtat atacaaatcc
     1261 ctccagtgac aactttccgc gccgacggac cagaaactcc cgaagaagaa aagggttact
     1321 ggtttcatgc ttatcaaccc gaagatcttt gtaccatcca taatccaatg ggagatttgc
     1381 aggattttat tgcattggtt aaagatgcta aaaaatttgg tatcgatatc attcctgatt
     1441 atacctttaa ctttatggga atttggggta gtggtaaaaa tgacctggat tatccctctg
     1501 ctgatatacg agcgaagatc agtaaagata tagaaggtgg tatccctggc tattggcaag
     1561 gtcaggtttt gattccattc attaaagatc cagtaacaaa agaacgtaaa caaatccatc
     1621 cagaagatat acatctcact gcaaaagact tcgaagcaag taaagataac atctctaagg
     1681 atgaatggga aaacctccat gcattaaaag aaaagcgttt aaatggaatt cctaaaacaa
     1741 cacccaaaag tgaccaggtt attatgttgc aaaatcaata cgttcgtgaa atgcgaaaat
     1801 atggcgtacg aggtttacgt tatgatgcgg caaaacactc aaaacatgaa caaatagaaa
     1861 gatcaataac cccaccgctt aaaaattata atgagcggtt acacaatact aacttattta
     1921 acccaaaata tcataaaaaa gccgttatga attacatgga atatctggta acttgtcagt
     1981 tggatgaaca acaaatgtca tcactgcttt atgaaagaga tgatttaagc gccattgatt
     2041 tttcattgct catgaagacg ataaaagcct tttcattcgg gggagatctc ctaacccttg
     2101 catcaaaacc gggttccaca atctcaagca tcccatcaga aagacggata ttgattaaca
     2161 ttaaccacga ttttcctaac aatggcaatc ttttcaatga ctttctattt aaccatcaac
     2221 aagatgaaca attagcaatg gcatatatgg ccgctctccc gttcagcaga cctttagttt
     2281 actgggatgg ccaggtatta aaatcaacga ctgaaattaa aaattatgat gggtccacgc
     2341 gtgtcggtgg tgaggcgtgg cttaataaag gttgctctac ctatcagcag ctctacaatg
     2401 aattccacgc attatatata gataaagcag gaatatggag cgcatttgag ggtgtatttg
     2461 caactaaaaa cgttctggcc tttagtcgtg gggattctgt gaacattaat cactctcctc
     2521 atgatggact agttataata aataaaggaa acgaagaagt tgaaggtacc tggcctaaca
     2581 aattgcaacc tggaatatat aaaaacatgg ggagtaatag cgttaacatt attattaata
     2641 atacccgaaa aattatcccc cctggtaaag tatttacgct tagaggcgga actctaaata
     2701 tcaatattcc tgggcgtagc gctcttcttt tagggaaaac aggagaaccg ccgaactatc
     2761 tctatttata atttaatgtt atatctgccc cgataaaacg gggcagataa tatgtttagt
     2821 ttactaacgg tcattttgca gtgaagccat ttactgtttt ttatcgacca gataatctgt
     2881 tctctaatgt taactccccc taacctgttg ctttagttat tcatttcctg tctcactttg
     2941 ccttaatact ctacgttaaa tgttactaat ttgttgcttt tgatcacaat aagaaaacaa
     3001 tatgtcgctt ttgtgcgcat ttttcagaaa tgtagatatt tttagattat ggctacgaaa
     3061 tgagcatcgc catgtcaccc tacatctcat aagaggatcg cttctgatga atgcactgac
     3121 cgccgtacaa aataacgctg tcgattcagg ccaggactat agcggattca ctctcatccc
     3181 gtcggcgcaa tccccgcgtc tgctggaact caccttcacc gaacagacga ccaaacagtt
     3241 tctcgagcag gttgccgaat ggcccgtaca ggcgctggag tacaaatcgt ttctgcgttt
     3301 tcgggtaggc aaaattcttg acgatctgtg tgcgaatcag ctgcaaccat tgctgttgaa
     3361 gaccctgcta aaccgtgctg aaggtgcgct gttgatcaat gcggtgggta tcgatgatgt
     3421 ggcgcaggca gatgagatgg tgaagctggc aacggcggta gcgcatctga ttggtcgctc
     3481 aaatttcgat gcgatgagcg gtcagtatta cgcgcgtttc gtggtgaaaa atgtcgataa
     3541 ctcagacagc tatctgcgtc agccgcaccg cgtgatggaa ctgcacaacg acggcacgta
     3601 tgtcgaagag atcaccgatt acgtgctgat gatgaaaatc gatgagcaaa acatgcaggg
     3661 tggaaattcg ttgctgctgc atctcgatga ctgggaacat ctggaccact atttccgcca
     3721 tccactggcg cgtcgcccga tgcgctttgc cgcgccgccg agcaaaaacg tcagcaaaga
     3781 tgttttccat ccagtgttcg acgttgatca acagggtcgc ccggtgatgc gctatatcga
     3841 ccagttcgtc cagccaaaag attttgaaga aggcgtgtgg ttgagcgagc tttccgatgc
     3901 cattgaaacc agcaaaggca ttctttctgt acccgttccc gttggcaaat tcctgttgat
     3961 aaacaacctg ttctggctgc acggtcgcga ccgctttact ccgcacccgg atctgcgccg
     4021 tgaactgatg cgtcagcgtg gctatttcgc ttacgccact caccactacc agacgcatca
     4081 gtaagcgcaa aggaattgag cggatgtatg attttgtgat tattggcggc ggcatcatcg
     4141 gcatgtcgac cgccatgcaa ctgattgatg tttatccgga tgcccgcatt gcgttgctgg
     4201 aaaaagagtc cggcccggcc tgtcaccaga cgggccacaa cagcggcgtg atccatgccg
     4261 gggtctatta cacgcccggc agcctgaagg cgcagttttg cctggcgggg aaccgcgcca
     4321 ccaaagcctt ttgcgatcaa aacggcattc gctatgacaa ctgcggcaag atgctggtcg
     4381 ccacgtctca gctcgaaatg gaacggatgc gcgccttatg ggaacgcaca gcggcgaacg
     4441 gtatcgagcg cgagtggata aacgccgtgg aactgcgcga gcgcgaaccg aatatcaccg
     4501 ggcttggtgg catttttgtg ccgtccagcg gcattgtcag ctaccgcgaa gtcacagcgg
     4561 caatggcaaa aatcttccag gccagaggcg gcgagattat ttataacgcc gaagtcagcg
     4621 ccctcagtga gcataaaaac ggcgtggtga tacgcacccg tcagggcggt gaatatgaag
     4681 catcgacgct gattagctgt tccgggctga tggctgaccg gctggtgaaa atgctcggcc
     4741 tcgaaccggg ctttatcatc tgcccgttcc gtggtgagta tttccgcctt gcgccggagc
     4801 ataaccagat tgttaaccac ctgatttacc ccattcccga ccccgcgatg ccatttttgg
     4861 gcgtgcatct cacccgcatg atcgacggca gcgtgaccgt cgggccaaac gcggtgctgg
     4921 ctttcaaacg cgaaggctat cgcaagcgcg acttctcatt tagtgacacg ctggagattt
     4981 tgagctcgtc ggggattcgc cgggtgctgc aaaaccatct acgctcagga ctgggcgaga
     5041 tgaaaaactc gctgtgcaaa agcggctacc tgcggctggt gcaaaagtat tgtccccggc
     5101 tttcgttaag cgatctccag ccctggcccg ccggtgtgcg ggcgcaggca gtatcgccgg
     5161 acggcaagct gattgacgat tttctgtttg tcaccacccc acgcacgatc cacacctgca
     5221 atgcgccctc cccggcagcg acatcagcaa ttcctattgg tgcgcatatt gtcagcaagg
     5281 tacaaacgct gttggcaagc cagagtaacc ccggacgcac gctgcgagcg gcacgtagtg
     5341 tggatgcctt acacgccgca tttaatcaat aacctttgaa aacaggacgt aacaatgaaa
     5401 cttaacgaca gtaacttatt ccgccagcag gcgttgatta acggggaatg gctggacgcc
     5461 aacaatggcg ggatcatcga cgtcaccaat ccggcgaacg gcgacaagct gggtagcgtg
     5521 ccgaaaatgg gcgctgatga aacccgcgcc gctatcgacg ccgccaaccg cgccctgccc
     5581 gcctggcgtg cgctcaccgc caaagaacgc gccaacattc tgcgcaactg gttcaatttg
     5641 atgatggagc atcaggacga tttagcgcgc ctgatgaccc tcgaacaggg caaaccactg
     5701 gccgaagcga aaggcgaaat cagctacgct gcctctttta ttgagtggtt tgccgaagaa
     5761 ggcaaacgta tttatggcga taccattccc ggtcatcagg ccgataagcg cctgattgtt
     5821 atcaagcagc cgattggcgt taccgcagct atcacgccgt ggaacttccc ggcggcgatg
     5881 attacccgta aagccggtcc ggcgctggcg gcaggctgca ccatggtgct gaagcccgcc
     5941 agtcagacac cgttctctgc gctggcgctg gcggagctgg cgattcgcgc gggcattccg
     6001 gctggcgtgt ttaacgtagt caccggttcg gcaggcgcgg tcggtaatga actgaccagc
     6061 aacccgctgg tacgcaaact gtcgtttacc ggttcgaccg aaattggccg ccagttaatg
     6121 gaacagtgcg cgaaagacat caaaaaagtg tcgctggagc tgggcggcaa cgcgccgttt
     6181 atcgtctttg acgatgccga tctcgataaa gccgtggaag gcgcgctggc ctcgaaattc
     6241 cgcaacgccg ggcaaacctg cgtctgcgcc aaccgtttat acgtgcagga cggcgtgtat
     6301 gaccgctttg ccgaaaaatt gcagcaggcg gtgagcaaac tgcacatcgg cgacgggctg
     6361 gataaaggcg tcaccatcgg gccgctgatc gatgaaaaag cggtagcaaa agtggaagag
     6421 catattgccg atgcgctgga gaaaggtgcg cgtgtggttt gcggcggtaa agcggatgaa
     6481 cgcggcggca acttcttcca gccgaccatt ctggtggacg tgccggccaa cgccaaagtg
     6541 tcgaaagaag agacgttcgg ccccctcgcc ccgctgttcc gctttaaaga tgaagccgat
     6601 gtgattgcgc aagccaatga caccgagttt ggccttgccg cctatttcta cgcccgtgat
     6661 ttaagccgcg tcttccgcgt gggcgaagcg ctggagtacg gcatcgtcgg catcaatacc
     6721 ggcattattt ccaatgaagt ggccccgttc ggcggcatca aagcctcggg tctgggtcgt
     6781 gaaggttcga agtatggcat cgaagattac ttagaaatca aatatatgtg catcggtctt
     6841 taactggaga atgcgaatga acagcaataa agagttaatg cagcgccgca gtcaggcgat
     6901 tccccgtggc gttgggcaaa ttcacccgat tttcgctgac cgcgcggaaa actgccgggt
     6961 gtgggacgtt gaaggccgtg agtatcttga tttcgcgggc gggattgcgg tgctcaatac
     7021 cgggcacctg catccgaagg tggtggccgc agtggaagcg cagttgaaaa aactgtcgca
     7081 cacctgcttc caggtgctgg cttacgagcc gtatctggag ctgtgcgaga ttatgaatca
     7141 gaaggtgccg ggcgatttcg ccaagaaaac gctgctggtt acgaccggtt ccgaagcggt
     7201 ggaaaacgcg gtaaaaatcg cccgcgccgc caccaaacgt agcggcacca tcgcttttag
     7261 cggcgcgtat cacgggcgca cgcattacac gctggcgctg accggcaagg tgaatccgta
     7321 ctctgcgggc atggggctga tgccgggtca tgtttatcgc gcgctttatc cttgcccgct
     7381 gcacggcata agcgaggatg acgctatcgc cagcatccac cggatcttca aaaatgatgc
     7441 cgcgccggaa gatatcgccg ccatcgtgat tgagccggtt cagggcgaag gcggtttcta
     7501 cgccgcgtcg ccagccttta tgcagcgttt acgcgctctg tgtgacgagc acgggatcat
     7561 gctgattgcc gatgaagtgc agagcggcgc ggggcgtacc ggcacgctgt ttgcgatgga
     7621 gcagatgggc gtggcgccgg atcttaccac ctttgcgaaa tcgatcgcag gcggtttccc
     7681 gctggcgggc gtcaccgggc gcgcggaagt gatggacgct gttgcaccgg gcggtctggg
     7741 cggcacctat gccggtaacc cgattgcctg cgtggctgcg ctggaagtgt tgaaggtgtt
     7801 cgagcatgaa aatctgctgc aaaaagccaa cgatctgggg cagaagctaa aagacggatt
     7861 gctggcgata gccgaaaaac acccggagat cggcgacgta cgcgggctgg gagcgatgat
     7921 tgccatcgag ctgtttgaag atggcgatca cagcaaaccg gacgcaaaac tcaccgccga
     7981 gatcgtggca cgcgcccgcg ataagggcct gattcttctc tcctgcggcc cgtattacaa
     8041 cgtgctgcgc atccttgtac cgctcaccat tgaagacgct cagatccgtc agggtctgga
     8101 gatcatcagc cagtgttttg ctgaggcgaa gcagtagcta tgtccgtttg taagagttaa
     8161 ccgttgctaa gggaatgata acgatgcagt aaaaaataat aacgatgagg cgaacgacaa
     8221 agttaaaaac gactgcggac agtcccctcg cccctctgga gagagggtta gggtgagggg
     8281 taaaacacgc ggctaaaccc tcccctggcc ctctccctaa gggagaggga gaaaaccctc
     8341 ccgctttcgt ttttaagatt ttgttgaccg tctcaattaa acgcgtgacc cggcgcggga
     8401 aatgtcgggg cgctctccca agtgacacac tttcgagagg attcaggatg gggcaatcat
     8461 cgcaaccaca tgagttaggc ggcgggctga aatcacgcca cgtcaccatg ttgtctattg
     8521 ccggtgttat cggcgcaagt ctgtttgtcg gttccagcgt cgccatcgcc gaagcgggcc
     8581 cggcggtatt actggcctat ctgttcgccg ggctactggt ggttatgatt atgcggatgt
     8641 tggcggaaat ggcggttgcc acgcccgata ccggttcgtt ttccacctat gccgataaag
     8701 ccattggtcg ctgggcgggc tataccatcg gctggttgta ctggtggttt tgggtactgg
     8761 ttatcccgct ggaagccaat atcgccgcta tgatcctgca ctcgtgggtt ccaggcattc
     8821 ccatctggtt attttccctc gtcattaccc ttgccttaac tggcagtaat ttattaagcg
     8881 ttaaaaacta cggcgaattt gagttctggc tggcgctgtg caaagtcatc gctatcctgg
     8941 cctttatttt ccttggtgca gtcgcaatta gcggttttta cccttatgcc gaagtgagcg
     9001 ggatctcaag attgtgggat agcggcggct ttatgcccaa cggtttcggt gcggtattaa
     9061 gcgcgatgtt gatcaccatg ttctcgttta tgggcgcaga aattgtcacc attgccgccg
     9121 cggaatccga cacgccggaa aaacatattg tccgcgccac caactcggtt atctggcgta
     9181 tttctatctt ctatttgtgc tctatttttg tcgtcgtggc attaataccg tggaatatgc
     9241 cggggctgaa agccgttgga tcttatcgct cggttctgga attgctcaat attccccatg
     9301 cgaaattaat catggactgt gtgatattac tttccgtaac cagttgcctg aactcggccc
     9361 tgtataccgc ctcaaggatg ctctactcct taagccgtcg cggtgatgct cccgcggtaa
     9421 tgggtaaaat caaccgcagt aaaaccccgt acgtggcggt gttactctcc accggcgcgg
     9481 cattcttaac ggtggtggtg aactattacg cgcctgcgaa ggtatttaaa tttctgatcg
     9541 acagctccgg cgctatcgcc ctgctggttt atttagtcat cgccgtttca cagttgcgga
     9601 tgcgcaaaat tctgcgagcg gaaggaagcg aaattcgctt gcgaatgtgg ctttatccct
     9661 ggctcacctg gctggtcatc ggctttatta cctttgtgtt ggtagtgatg ctattccgtc
     9721 cggcgcaaca gttagaagtg atctccaccg gcttattagc gatagggatt atctgtaccg
     9781 tgccaattat ggctcgctgg aaaaagctgg tattgtggca aaaaacaccc gttcataata
     9841 cgcgctgata atgatcagga gtcacaccat gaccattacg tctctggatg gctatcgctg
     9901 gctgaagaac gatattattc gcggtaattt tcaaccggat gaaaaattac ggatgagttt
     9961 gctgacatcg cgttatgcac ttggcgttgg gccgttgcgg gaagcacttt ctcaactggt
    10021 ggcggaacgg ctggtcacgg tggtgaatca aaaagggtat cgggtggcgt ctatgtcaga
    10081 gcaggagctg ctcgatattt tcgatgcccg cgccaatatg gaagcgatgt tagtgagtct
    10141 ggcgattgcc cgcggtggcg atgagtggga ggcagacgtt ctcgcaaaag cgcatctgct
    10201 cagtaagctg gaagcctgtg acgccagcga gaaaatgctt gatgagtggg atctgcgtca
    10261 tcaggcgttt catacagcaa ttgtggcggg ctgtggttct cactatttgc tgcaaatgcg
    10321 tgaacggttg tttgatctgg cggcgcgcta tcgatttatc tggctgcggc gaacggtgct
    10381 ttcggtagaa atgctggagg ataaacacga tcagcaccag accctgactg cggcggtact
    10441 ggcgcgagat accgcgcgcg ccagtgagtt aatgcgccag catttactga cgccaattcc
    10501 cattatccag caggcgatgg ctggcaatta actactcttc cggaatacgc aacacttgcc
    10561 ccggataaat tttatccggg ctttttagca tcggtttatt cgcttcgaag attttattgt
    10621 acagattagc gttaccgtag acctgtttgg aaatggcact cagagtgtcg ccagacttaa
    10681 cggtataaaa ctggctggca gtggctggtg tcgccgtttt cacctgatca tcaacactgg
    10741 caataccgga aatattcccc accgcaacaa ggattttctc cttcgcctcc tgactcaggc
    10801 cgtcaccagt gaccgtcgct ttgccgtcgg caatttgaat gttcacttta tcagcatcag
    10861 gtataccggt tttgctcaga tgctcctgca ccttcttcgc ctgatcgtct ttatcgtgct
    10921 gacctgtaac cgcgtcccag agtttttctc cggcatcttt cacaaaattg aacagaccca
    10981 tagctacctc attcgttaac ggaaacactc agaaagtgta gcagaggaac gcggcgcgtt
    11041 aacgccgcgg gggtgagcac aggtttaatc gcgggtttgc acccagaacg cgtgaatcaa
    11101 accggggata tacccaagca gtgtcagcag aatattaata atgaacgccc aaccgaaccc
    11161 tttaccgagc agcacgccga gcggcggtag aatgatggtg atgacgattc tccagaaacc
    11221 catatgtact ccctataaga aaattactca ttgtttaaaa agagatttta tctcttaagc
    11281 gtagtaattt tagcggagcc tgccagtttc agcttacgct tttactctca tttacgctgc
    11341 tgaggctggt atatgttgca tttttagttt agagcttact taatttagaa aatacttaaa
    11401 taaatatgac tgaactcgcg caattacagg ccagtgccga acaggcagcg gccttattga
    11461 aagcaatgag ccatcctaaa cgattgctga ttctgtgcat gcttagcggt tcccccggca
    11521 ccagcgcggg agagctgacg cgcattaccg gactgagtgc ctctgcgaca tcacagcatc
    11581 tcgctcgtat gcgggacgaa gggcttatcg acagccaacg ggatgcccaa cgcattctat
    11641 attccattaa aaatgaggcg gtaaatgcca ttatcgccac cctgaaaaat gtctattgtc
    11701 cgtaaggagt cactatggct ttgacaacca tttcgccgca tgatgcacaa gaattaatcg
    11761 cacgcggcgc aaagttaatc gatattcgtg acgctgatga atatcttcgt gaacatattc
    11821 ctgaagcaga tctggctcca ttatccgtgc tggaacagtc aggtcttcca cctaaattac
    11881 gtcgcgagca aattatattc cactgccagg caggtaaacg caccagtaat aacgccgata
    11941 aattagcagc gattgccgcc cccgcagaaa tatttttact cgaagatggg attgatggct
    12001 ggaaaagagc tggattgcca gtagcggtaa ataaatctca acccttgccg ttaatgcgcc
    12061 aggtgcagat cgctgcgggt ggtttaatat taatcggcgt tgtactgggt tataccgtaa
    12121 atagcggttt cttcttatta agtggctttg ttggtgccgg gttactgttt gcaggaatca
    12181 gcggtttttg tggaatggca aggttgttag ataagatgcc gtggaaccaa cgagcttgag
    12241 aagcgacgcc ggacgcgccc tagcagcgac atccggcctc agtaattaga tcaggaaatc
    12301 gtcgagagat ttaccttctg ccagcgcctg agcaattggc ttcggtgtac ggccctgacc
    12361 ggtccaggtt ttagtttcac cgttaacatc ggtgaattta tatttcgccg gacgcggctg
    12421 gcgtttttta ccagcgcgtg gtgcagcagc agagctatta cccaataact cttccgggtt
    12481 aattccgtca gccttcatca gctccagcca ggtgctaatt ttttcctgac gctcagccag
    12541 ttcacgctgc tgctgttctt cttcttcacg tctttcttta gtgacaaccc tgaatttttc
    12601 gagcatttct tcaagaacgt caatggagaa ttcgcgagcc atcgcacgga gggtgcgaat
    12661 gttatttaaa ctttgtaaca ttacggacat aataaagaaa accttttaac gccaaaacaa
    12721 aaaagtattt cgtcgcgtag tgtaagcgat cacttattat atttccacaa aatttaatct
    12781 atttacagtc ctgcgcgaga ttatttcagc tgttattttc tgaaactctc agaataattt
    12841 caagattata tattagccat tttgatgagt aatttagatt attggtgaac agaacggcgc
    12901 tctctgctcg ctaattccgc aagcctgaca aaaccgaagt gaataaaaat gcaatataac
    12961 agaataaaag
//
LOCUS       DAKISA010000118        12732 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.118, whole genome shotgun sequence.
ACCESSION   DAKISA010000118 DAKISA010000000
VERSION     DAKISA010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12732
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.118"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            261..476
                     /gene="bdm"
                     /locus_tag="OVW45_003543"
     CDS             261..476
                     /gene="bdm"
                     /locus_tag="OVW45_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HCV5527904.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEQGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            578..715
                     /gene="sra"
                     /locus_tag="OVW45_003544"
     CDS             578..715
                     /gene="sra"
                     /locus_tag="OVW45_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HCV5527905.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            872..2569
                     /gene="maeA"
                     /locus_tag="OVW45_003545"
     CDS             872..2569
                     /gene="maeA"
                     /locus_tag="OVW45_003545"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCV5527906.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            2703..3705
                     /gene="adhP"
                     /locus_tag="OVW45_003546"
                     /pseudo
     CDS             2703..3705
                     /gene="adhP"
                     /locus_tag="OVW45_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            3851..4135
                     /locus_tag="OVW45_003547"
     CDS             3851..4135
                     /locus_tag="OVW45_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCV5527907.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(4363..5016)
                     /gene="fdnI"
                     /locus_tag="OVW45_003548"
     CDS             complement(4363..5016)
                     /gene="fdnI"
                     /locus_tag="OVW45_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCV5527908.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(5009..5893)
                     /gene="fdnH"
                     /locus_tag="OVW45_003549"
     CDS             complement(5009..5893)
                     /gene="fdnH"
                     /locus_tag="OVW45_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCV5527909.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEERVAKLKARGYEHAGVYNPKG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5906..8953)
                     /gene="fdnG"
                     /locus_tag="OVW45_003550"
     CDS             complement(5906..8953)
                     /gene="fdnG"
                     /locus_tag="OVW45_003550"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8366..8368),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCV5527910.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            9185..10066
                     /gene="yddG"
                     /locus_tag="OVW45_003551"
     CDS             9185..10066
                     /gene="yddG"
                     /locus_tag="OVW45_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCV5527911.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            10325..11425
                     /gene="ompC"
                     /locus_tag="OVW45_003552"
     CDS             10325..11425
                     /gene="ompC"
                     /locus_tag="OVW45_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCV5527912.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            11589..12071
                     /locus_tag="OVW45_003553"
                     /pseudo
     CDS             11589..12071
                     /locus_tag="OVW45_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12186..>12732
                     /locus_tag="OVW45_003554"
     CDS             12186..>12732
                     /locus_tag="OVW45_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCV5527913.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAH"
BASE COUNT         3345 a         3161 c         3006 g         3220 t
ORIGIN      
        1 acggcgaaaa tattacttct gcacttaaat taaattccca caatagataa ccgggaatat
       61 atatctcaag agatattgca tcaatatcaa tcaactcacc acaataataa tacatatcaa
      121 tatcatggag ttaaaatata aaaatcatca accaggacta atcttaacaa cgaagcggca
      181 aatatttgcc atgctgaatg tgctttataa atctgcgatc cgtagcagac accataaata
      241 cacagacacg gagaatcact atgtttactt attatcaggc agaaaattca acagcagaac
      301 ccgctctggt taatgccatt gagcaaggcc ttcgggcaga gcaaggtgtt gtcactgaag
      361 atgacattct catggagctg accaaatggg tggaagcttc tgataacgac atcctcagtg
      421 atatctacca gcaaacgatc aattatgtgg tcagtggcca gcaccctacg ctttaaggtg
      481 ctatgcttga tcggcaacct aatttagggg tttagcacgt gtttcttcgc tacggcgatg
      541 ttgtccttaa aactagcaac aggattgagg agttaaaatg aaatcgaacc gtcaggcacg
      601 tcatattctt ggactggacc ataaaatttc taaccagcgc aaaatagtta ccgaaggtga
      661 caaatccagc gtggtaaata acccaaccgg cagaaaacgc cccgctgaaa agtaattcat
      721 aaccatcagt cctcaatgac gattaaacac cattgcctgc gcaatggtgt ttttgttttt
      781 atctgcttta tacttggggc cgacgccctg gcggtaaagc aaagacgata aaagcgtgcc
      841 agggatggat attcaaaaaa gagtgagtga catggaacca aaaacaaaaa aacagcgttc
      901 gctttatatc ccttacgctg gccctgtact gctggaattt ccgttgttga ataaaggcag
      961 tgccttcagc atggaagaac gccgtaactt caacctgctg gggttactgc cggaagtggt
     1021 cgaaaccatc gaagaacaag cggaacgagc atggatccag tatcagggat tcaaaaccga
     1081 aatcgacaaa cacatctacc tgcgtaacat ccaggacacc aacgaaaccc tcttctaccg
     1141 tctggtaaac aatcatcttg atgagatgat gcctgttatt tataccccaa ccgtcggcgc
     1201 agcctgtgag cgtttttctg agatctaccg ccgttcacgc ggcgtgttta tctcttacca
     1261 gaaccgccac aatatggacg atattctgca aaacgtaccg aaccataata tcaaagtgat
     1321 tgtggtgact gacggtgaac gtattctggg gcttggtgac cagggcatcg gcgggatggg
     1381 cattccgatc ggtaaactgt cgctctatac cgcctgtggc ggcatcagcc cggcgtatac
     1441 ccttccggtg gtgctggatg tcggaacgaa caaccaacaa ctgcttaacg atccgctgta
     1501 tatgggctgg cgtaatccgc gtatcactga cgacgaatac tatgaattcg ttgatgaatt
     1561 tatccaggct gtgaaacaac gctggccaga cgtgctgttg cagtttgaag actttgctca
     1621 aaaaaatgcg atgccgttac ttaaccgcta tcgcaatgaa atttgttctt ttaacgatga
     1681 cattcagggc actgcggcgg taacagtcgg cacactgatc gcagcaagcc gagcggcagg
     1741 tggtcagtta agcgagaaaa aaatcgtctt ccttggcgca ggttcagcgg gatgcggcat
     1801 tgccgaaatg atcatcgccc agacccagcg cgaaggatta agcgaggaag cggcgcggca
     1861 gaaagtcttt atggtcgatc gctttggcct gctgacggac aagatgccga acctgctgcc
     1921 tttccagacc aaactggtgc agaaacgcga aaacctcagt gactgggata ccgacagcga
     1981 tgtgctgtca ctgctggatg tggtgcgcaa tgtaaaacca gatattctga ttggcgtctc
     2041 aggacagacc gggctgttta cggaagagat catccgtgag atgcataaac actgtccgcg
     2101 tccgatcgtg atgccgctgt ctaacccgac gtcacgcgtg gaagccacac cgcaggacat
     2161 tatcgcctgg accgaaggta acgcgctggt cgccacgggc agcccgttta atccagtggt
     2221 atggaaagat aaaatctacc ctatcgccca gtgtaacaac gcctttattt tcccgggcat
     2281 cggcctgggg gttattgctt ccggcgcgtc acgtatcacc gatgagatgc tgatgtcggc
     2341 aagtgaaacg cttgcacagt attcgccgct ggtgctgaac ggcgaaggtc tggtactacc
     2401 ggaactaaaa gatattcaga aagtctcccg tgcaattgcg tttgcggttg gcaaaatggc
     2461 gcagcagcaa ggcgtggcgg tgaaaacctc tgccgaagct ttgcaacaag ccattgacga
     2521 taatttctgg caagccgaat accgcgacta ccgccgtacc tccatctaag cctgcgcccg
     2581 gtagtgaagg ctaccgggca attttcctct ccctttttca gatctcatcc atactgggta
     2641 gtggcgaata aatctcattt gcctcacctg ctatgcagaa catcatccga aaaggaggaa
     2701 ctatgaaggc tgcagttgtt acgaaggatc atcatgttga cgttacggat aaaacactgc
     2761 gctcactgaa acatggcgaa gccctgctga aaatggagtg ttgtggtgta tgtcataccg
     2821 atcttcatgt taagaatggc gattttggtg acaaaaccga cgtaattctg ggccatgaag
     2881 gcatcggtgt ggtggcagaa gtgggtccag gtgtcacctc attaaaacca ggcgatcgtg
     2941 ccagcgtggc gtggttctac gaaggatgcg gtcattgcga atactgtaac agtggtaacg
     3001 aaacgctctg ccgttcagtt aaaaatgccg gatacagcgt tgatggcgga agagtgcatc
     3061 gtggtcgccg attacgcggt aaaagtgcca gatggtctgg actcggcggc ggccagcagc
     3121 attacctgtg cgggggtcac cacctacaaa gccgttaagc tgtcaaaaat tcgtcccggg
     3181 cagtggattg ctatctacgg tcttggcggt ctgggtaacc tcgccctgca atacgcaaag
     3241 aatgtcttta acgccaaagt gatcgccatt gatgtcaatg atgagcagtt aaaactggca
     3301 accgaaatgg gtgcagattt agcgattaac tcacgcaccg aagacgccgc caaaattgtg
     3361 caggagaaaa ccggtggcgc tcactctgcg gtggtaacag cagtagctaa agctgcgttt
     3421 aactcggcag ttgatgctgt ccgtgcaggc ggtcgtgttg tggctgtcgg tctgccgccg
     3481 gagtctatga gcctggatat cccacgtctt gtgctggatg gcattgaagt ggtcggttcg
     3541 ctggtcggca cgcgccagga tctaaccgaa gccttccagt ttgccgccga aggcaaagtg
     3601 gtgccgaaag tcgccctgcg tccgttagcg gacatcaaca ccatctttac cgagatggaa
     3661 gaaggcaaaa tccgtggccg tatggtgatt gatttccgtc actaagaggt ctttgctgcg
     3721 actcccatgt tcgggtcgca gcatcgcaca ctctccaaca tgaaatggct aaatggatta
     3781 ttgttgcgta ggaaggaaca ataatgatag tgttcccaga gaaggaacgc tactgagagt
     3841 aaagacacta atgaaaatgg ccaatcatcc ccgcccgggg gacattattc aggaatcact
     3901 ggacgaactt aatgtcagcc tgcgcgagtt tgccagagca atggaaattg cgccctcaac
     3961 ggcaagccga ttgctgaccg gaaaagcagc tttgacgcca gaaatggcaa ttaaactttc
     4021 cgtggtgatc ggcagttcgc cgcaaatgtg gctgaatctg caaaacgcct ggagtctggc
     4081 agaggcagaa aaaacggtgg atgtgtcgcg attacgccgt ctggttacgc aataaaaaat
     4141 ggcaccttat aaagcgccat ttgaaactga tgacaaacgc aacattgcct gatgcgctac
     4201 gcttatcagg cctacacgtt cacttcaatc tattgtattt gaagggtttt catgccggat
     4261 aaggcattca cgacgcatcc gggatgaaca aagcccacat tgtctacaat atgaaaatgg
     4321 cgccttttac agcgccattt tcagcttaaa gttcaaaaga gattatatcc cttcttcact
     4381 ctcttttttc gcttctgcct tctcgatttc acgataccag cgcggatggt gtttcttcgc
     4441 ccagcgacgg ctcaccttcc cttcgatcat ccctttaatc gaccctttca cccagaatgc
     4501 catatacata tggatcagga tggcgtggat caggatgata cccgcagccg cgtggatcag
     4561 caggctgtag cgaacaacct gcatcgggaa gtactgcgca aagtacggac gccagataat
     4621 caccccggtc accagcagca cgaaaatcat gctcatgatc gaccagaaca tcatcttttg
     4681 cccggcgttg tacttaccaa catccgccac tttgtgctca ttgcctttta atacttcgac
     4741 aatgttcaac agccacggaa tatctttctt atccgggatg ttgtgatgca caaaacgcac
     4801 aaacataaac atcagtgcga cgaaaatcgc aatgccgaag aacgggtgca aaatgcgtcc
     4861 catctgcggc gtaccgaagg tttgcgtcag ccattgcagc gtcgggaaga agaacgaaat
     4921 cccggacagc gccaccagga agaagcaaat caccacggtc cagtgacagg cgcgatcaat
     4981 aaatttggtg cgcacaatca ttttcgactt actcatgatg atcctcctcg tcatcgtcca
     5041 cttccttatt cgggccaata ccgatgtagt ggaaaatcaa cccggcaaaa gtggcaataa
     5101 agccagccgc tgccagcggt ttcaacgcgc ctttccacag gcttaccgag gtgtcgatct
     5161 tcggatcttt cggcagaccg tgatacagct ccggctgatc ggcgtgatgc agcacgtaca
     5221 taacgtgcgt accaccgacc cctttcgggt tgtagacgcc agcatgttcg taaccacgcg
     5281 ctttcagttt cgccacgcgc tcttccgcca gctccagcat ctccttcttg gtgccgaagt
     5341 ggatagcccc ggtcggacag gttttcacac aagccggttc ctggccgacg ctgacgcgat
     5401 cgacgcagag cgtgcattta tatacccggt tatcctcttt gttgaggcgc ggaatattaa
     5461 acggacaccc ggcaatgcag taaccacagc cgatgcagtt ttccgactgg aaatcgacaa
     5521 tcccgttagc gtactgaatg attgcaccag cagacgggca cgccttcagg cagccgggat
     5581 cttcacagtg catacagccg tctttacgga tcagccactc cagcttgccg ttctgttcgg
     5641 tttcgctaaa gcgcatcacc gtccaggact tggcgctcag atcggcgggg ttatcgtaaa
     5701 ccccgacgca gtgccccact tcatcacgga tgtcgttcca ctccgaacac gccacctgac
     5761 aggctttaca gccgatacag gtggaaacgt cgataagttt tgcgacttct gctttgtaat
     5821 cacgcacctg agaaggcggc gtgatggagt tagttgcgga ccttttgata atatcctgcg
     5881 tttccatagc catctgttcg cccccttacg ccttctcgat gttgactaag aacgctttat
     5941 attccggcgt ttgcgagttt gcatcaccga cattcggcgt cagagtgtta gcgatataac
     6001 ctttacgcgc gacaccctca aagccccagt ggattggaat acccaccgtt tcaacctgct
     6061 gaccgtttac attcagcggt ttcagacggc gcgttaccac cgccaccgcg cggataaagc
     6121 cacgcttgct tgagacagtg acacgatcgc cattattaat gcctttcgcc gccgccagcg
     6181 tttcgctgat ttccacaaac tgttccggct gagcaattgc gttgagcaat gcgtgcttgg
     6241 tccaggtgtg gaagtgctcg gtcagacgat aggtcgtacc cacatacggg aactgctctt
     6301 ttttacccat ccgcagcgcg tcttgttcat acagacgaac aaccgggtta gacaccacgt
     6361 tcggatgcag cgggttagtg cccagcggcg tttcaatcgg ctcgtagtgt tccgggaacg
     6421 gaccttccgc cattttgttg atggcaaaca ggcgtcccat cccttccggc tgcatgataa
     6481 acggcccggt tggcgtaccc ggtgcggcat tgccgaagtc cggaatatcg ttacccgtcc
     6541 acttgctgcc gttccactgg atcagcatcc gtttcggatc ccacggttta ccattgatat
     6601 ccgccgaagc acggttgtag agcacgcgac ggttgagcgg ccacgcccag gcccatccca
     6661 gcgtattccc cagaccggat gggtcggagt tatcgcggtt agccatctgg ttgccctgct
     6721 ctgtccagct accggtgtag atccagcaag aagatgcggt tgtaccgtca tcacgcagat
     6781 gcgcaaagct actcagcaac tgacctttct tcgcaatcag cacgccatta gcgtcataga
     6841 gatcttccag cgcatagccg ttattctctt tcgccacttc gtcagattgc ggttcgtgcg
     6901 gctgcttgta gttccagctc atcttcatca gcggttctac gcctttacca ccttcggcct
     6961 ggtacagctc gcgcagatga tggtagatac ccgccagaat ttcgccgtcg ttacgcgctt
     7021 cgcccggcgc gtcctgacct ttccagtgcc actgcaacca gcgaccggag ttagcgatag
     7081 aaccatcttc ttcggcaaag caggtcgaag gcagacggaa tacttcagtc tgaatagacg
     7141 ccggatcgac atcgttcgac tcgccgtggt tctgccagaa ggtagaggtt tcagtcacca
     7201 gcggatcgat aaccaccatg tacttcagct tgctcaggca gctcaccact ttgtttttgt
     7261 ccgggaagga cgcaaccggg ttaaagccct ggcagaagta accggtgact ttgccttcgt
     7321 ccatcatgtt gaaatacttg atgacgtcgt aggtctggtc ccacttcggc agccagtcat
     7381 agccccagtt gttctctttc tgcgcggcat cgccatagaa agatttcatc aggctaacga
     7441 agaacttcgg atagttgctc cagtagttca cctgatcagc cagcgtcgct ttcggcgtgt
     7501 tcgcttccag atacgactgc aaatcaacct gtttttctga cggaagcgtc agataacctg
     7561 gcaggctggt agagagcaga cctaagtcag tcagcccctg aatgttggag tgaccacgca
     7621 atgcgttcac gccgccaccg gccataccca tgttaccgag cagcaactgg atcatcgcca
     7681 tagtacggat gttctgcgca cccacggtgt gctgcgtcca gcccagcgcg tacaggaagg
     7741 tggttgtgcg atccggtgcg ctggtggagg ccagcacttc acacactttc aggaagtcgg
     7801 cttttggcgt accgcagatg ttttcaacga cgtccggcgt gtagcgggaa acgtgcgctt
     7861 tcagcaggtt ccacacacag cgcggatgag tcagtgtttc atcgcgtttt gcatagccgt
     7921 tttcatcgaa ctgatagttc caggacgact tatcatactg gcgtttttca gcgtcgtagc
     7981 cgctgaacag gccgtcttcg aaagcaaaat catcacgcac cagcaggctg gcgttggtgt
     8041 aatgtttaac gtattcggcg ttgattttgt tgttttcgat caggtagcgc aaaacgccag
     8101 acaggaacgt aatgtccgta ccggaacgaa taggtgcgta aatatccgcc acagaagcgg
     8161 tacgcgtaaa acgaggatcg acaacgatca aggttgcatc gttgttgttt ttcgcttcca
     8221 tcgcccagcg gaaaccgacg ggatgcgctt cagcagcgtt accgcccatt accattacga
     8281 cgttagcgtt tttgatatcc acccagtggt tggtcatcgc accgcgacca aatgttggag
     8341 caagacttgc taccgttggt ccgtgtcaga cgcgcgcctg gttgtctacc gccagcatcc
     8401 cgagggagcg ggcaaatttc tgggtcagca tcccggtttc gttgctggca ccggaggcac
     8461 acagcatacc ggtagaaagc caacggttta ccgttacgcc ctgctcgttc ttttcaataa
     8521 agttagcgtc acggtcagct ttcatcagct tcgcaatacg ggagaatgct tcttcccagc
     8581 taatgcgctg ccatttgtca gaacctggcg cacgatattc cgggtagcgc agacggtttt
     8641 cgctgttgac gtaatccagc aaaccggccc cttttgggca cagcgcacca cggcttaccg
     8701 gatgatccgg gtcaccttca atgtgataaa tcgcttctct ggcgtttttc gcgccatcac
     8761 ccaggctata catcaatagc ccgcaaccta cggaacagta tgtgcaggtg ttacggatct
     8821 ctttagcgcg taataatttg tagtttcgcg cctgagccag tgcttgcttc ggggcaaagc
     8881 ccaatgccgc taccgttgtt ccagccatac cgcccgcgca gattttaaaa aattgtctgc
     8941 gactgacgtc cattgctttc ctcttttttc aggggtatta ctgcgcgagg aaactaacag
     9001 taaaattctg aatctttttg cgcgaaatca aaaagaacac gtgtatgcca caaaataatc
     9061 cgcctgcgca aattaaccta cctcaatagc ggtagaaaaa cgcaccactg cctgacaggc
     9121 cagttaaaaa aatgctataa aattcagctt aatttttaac ggcaagagag acaaaacagc
     9181 gagcatgaca cgacaaaaag caacgctcat agggctaata gcgatcgtcc tgtggagcac
     9241 gatggtagga ttgattcgcg gtgtcagcga ggggctcggt ccggtcggcg gcgcagctgc
     9301 tatctattca ttaagcgggc tgctgttaat cttcacggtt ggatttccgc gtattcggca
     9361 gattccgaaa ggctatttac tcgccgggag tctgttattc gtcagctatg aaatctgtct
     9421 ggcgctttcc ttagggtatg cggcgacccg tcatcaggcg attgaagtgg gtatggtgaa
     9481 ctatctgtgg cccagcctga caattctctt tgccattctg tttaatggtc agaaaaccaa
     9541 ctggctgatt gtaccaggat tattattagc cctggtcggc gtctgctggg tgttaggcgg
     9601 tgagaatggg ttacattatg atgaaatcat caataatatc accaccagcc cattgagtta
     9661 tttcctggcg tttattggtg cgtttatctg ggcagcctat tgcacagtaa cgaataaata
     9721 cgcacgcgga tttaacggaa ttaccgtttt tgtcctgcta acgggagcat gtctgtggat
     9781 ttactatttt ctcacgccac aaccagaaat ggtatttagc acgcccgtca tgattaaact
     9841 catctctgcg gcatttacct taggatttgc ttatgctgca tggaatgtcg gtatattgca
     9901 tggcaatgtc accataatgg cggtaggctc gtattttacg cctgtacttt cctcagcgct
     9961 tgcagccgtg ctgctcagcg ccccgctgtc gttctcgttc tggcaaggcg cgctaatggt
    10021 ctgcggcggt tccctgctct gctggctggc gacacgtcgt ggttaaaata attgccgggt
    10081 ctacggcccg gcagttaatc agataaattc cgacaatatt aaaaaataaa tttcaaatac
    10141 cagtaattca ccttatatct catttcgact tcgatcacat atcgataaat aatattattt
    10201 catctatttt tctcattgtt tttaatcacc attctatttt aaaaacagta tgtcatttat
    10261 aaatgacgcc gtttaaaatt cgtttagaaa atacgtttaa cgataattaa aaggattctg
    10321 ttaaatgaaa ttaaaaatag ttgcggtggt tgtaactggt ttgttagctg cgaacgtagc
    10381 acacgctgcc gaagtctata acaaggatgg taataaactc gacctttatg gcaaggttac
    10441 cgctctacgt tattttactg atgataagcg tgacgatggt gataaaactt atgcccgtct
    10501 cggctttaaa ggagaaacgc aaatcaatga tcaaatgatt ggttttggtc actgggaata
    10561 tgattttaaa ggctataacg atgaagccaa cggctcgcgc ggcaacaaga cccgtctggc
    10621 ctatgctggt ttaaaaatta gtgaatttgg ctctctggac tatggtcgta actacggtgt
    10681 cggctacgac attggttcat ggaccgatat gttgccagaa tttggtggcg atacctggag
    10741 tcagaaagat gtcttcatga cataccgtac caccggtcta gcaacctatc gcaactacga
    10801 tttctttggc ttaattgaag ggctgaactt tgccgcgcaa tatcaaggca aaaatgaacg
    10861 tactgacaac agtcatcttt atggtgctga ctacacgcgt gccaacggtg acggtttcgg
    10921 tatctcctca acttatgttt atgatggctt tggtatcggt gcggtatata ccaaatccga
    10981 tcggacaaat gcgcaggaaa gagccgctgc taatcctctc aatgcctccg gtaagaatgc
    11041 agaactatgg gccacaggta taaagtatga tgccaacaac atctactttg cagctaatta
    11101 cgctgaaaca ttaaacatga ccacctatgg ggatggttat atctctaaca aagcacaaag
    11161 ttttgaagtg gtggcgcaat atcaattcga cttcggcttg cgcccatcac tcgcttacct
    11221 gaaatcgaaa ggcagagatc tgggccgcta cggcgaccag gacatgattg agtatatcga
    11281 cgttggtgcg acgtatttct tcaacaaaaa tatgtcgacc tatgttgatt ataaaatcaa
    11341 cctgattgat gaaagcgact ttacccgtgc cgtagatatt cggaccgata acatcgtcgc
    11401 tacgggcatt acctatcagt tctaacgtcc tctcctgacc agcccttctc tgggctggtt
    11461 tttgatttaa gtgctgctgt atgactcctt aagtaaaaat gagcaatttt tgatatttct
    11521 ggtcattcac ctcaattaat attattccat atcctccggg gacatcttac tataatattt
    11581 attgatgagt gattatgacc aatattaata cagcttgtgt aaaaaataat gccagttatc
    11641 aacttaataa cgcattacct aacaaagaaa ccatctccag caatttttgt gaacgactag
    11701 aacaatgggg taataaatcg gttaataatg gtgaagaaag agcaattgcc gtggagcgaa
    11761 ttaaagaagc ttacaattcg aacatggcat ctcttgattt gtcatatctt gatttgagtg
    11821 aattaccgcc tatcccctcg acagttaata cgttaaatct ggaaaacaat tgtctaactt
    11881 gtcttgactt agctgacaat gccagtctcg ttaatatcaa cctcagcgtt aataaaatta
    11941 acacgataac ctttccaaaa gaatcaaaac tggaaaacat ttatattgat cataacaatc
    12001 tggaaagttt agatttaaaa aatcagcatt cattggttaa tctggaagcg caaaataaca
    12061 aactgacaaa aattaatatt tctgatagtt ataaactgaa atttcttaat cttgattata
    12121 acaagctagc atcgctggat gtctcccggc aagaatctct gattgagtta agtgcccatc
    12181 acaatatgat cactgacctt atattacaca atcatcccag gatgaaaaca atcactttaa
    12241 acgacaacca tattgcacat ttaaacgcca aaaacactac aaaactggaa tatttaaact
    12301 taagcaataa caatttactg ccaaccaatg acattgatca actaatatca tcaaaacatc
    12361 tttggcatgt attagttaac ggcatcaaca atgatccact tgcacaaatg cagtactgga
    12421 ctgcagtaag aaatataatt gatgacacta atgaagtgac cattgattta tcaggactta
    12481 atttaaccac tcaaccacca gggctgcaaa acttcacatc tatcaatctt gataataacc
    12541 aactcacaca ttttgatgca accaactacg atagactcgt aaagctaagt ctgaatagta
    12601 atgctcttga gtcaataaat tttcctcaag gcagaaatgt aagtattaca catatatcta
    12661 tgaataataa tgctctcaga aatattgata tagataggct ttcatcagtt acttatttta
    12721 gtgcggcaca ta
//
LOCUS       DAKISA010000119        12361 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.119, whole genome shotgun sequence.
ACCESSION   DAKISA010000119 DAKISA010000000
VERSION     DAKISA010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12361
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.119"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..1056)
                     /locus_tag="OVW45_003555"
     CDS             complement(<1..1056)
                     /locus_tag="OVW45_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HCV5527914.1"
                     /translation="MSKVFICAAIPDEKATKEEGAKPVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESEPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMCPEIKLHAIGWIKHKCKPGAKWPEIQAEMRIWKKCREGERKETGKYTSVVDLARA
                     RANQQHTENSTGKISPAIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGTNQSATD"
     gene            complement(1150..1341)
                     /locus_tag="OVW45_003556"
     CDS             complement(1150..1341)
                     /locus_tag="OVW45_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HCV5527915.1"
                     /translation="MNTAFALVLTVILNTGEPAELVTGVYNSMKECVAAAAEQKIPGN
                     CYPVDKIIHQDNKEVPAGL"
     gene            complement(1338..1526)
                     /locus_tag="OVW45_003557"
     CDS             complement(1338..1526)
                     /locus_tag="OVW45_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HCV5527916.1"
                     /translation="METLLPNVNTTEGCFEIGVTISNTVFTEDAINKRKHERESLNKI
                     CIVSMLARLRLMQKGCWR"
     gene            complement(1537..2391)
                     /locus_tag="OVW45_003558"
     CDS             complement(1537..2391)
                     /locus_tag="OVW45_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCV5527917.1"
                     /translation="MLNVAIENQNGWNYSAPAPYKTGAGRGNPNVTRAHSRAEAVFLC
                     VMHSSIQIMVGCAGQSQDWPGSRVTGISTPVRLTTLMVVENLGGELINLSLEDAIMAT
                     IPALSHPDVTIENGRAVTTSIAIAEFFGKRHDNVLRAIANTECSPEFNALNFEDVTYT
                     DAKGEKRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPTDKI
                     IPGDGRTLVVHFDERGNIRFTETVPDGARVCTMETFRFYLEEQGWTLVNRGAIKNMTV
                     EQLLKIHC"
     gene            2823..3023
                     /locus_tag="OVW45_003559"
     CDS             2823..3023
                     /locus_tag="OVW45_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5527918.1"
                     /translation="MTKEEFVSYIFDKTVEMYAATYGSCNPLNKPEGKDDFDKIYRFL
                     EDRYIKSLEDAGIKSPVKSPLS"
     gene            complement(2992..3369)
                     /locus_tag="OVW45_003560"
     CDS             complement(2992..3369)
                     /locus_tag="OVW45_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5527919.1"
                     /translation="MRLTMEFKDLPSDVQKTAAHTLHSVLREIGKDIASEPAKDLARK
                     IKTAFVELYNVGTDSETVETKTVSSPIFSLEPSVLSGEICTEISSELLPVIREAIRRR
                     GLDGSYDHDVLQVLRTMVTSLGI"
     gene            complement(3369..3521)
                     /locus_tag="OVW45_003561"
     CDS             complement(3369..3521)
                     /locus_tag="OVW45_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCV5527920.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARCW
                     LARNTD"
     gene            complement(3798..4088)
                     /locus_tag="OVW45_003562"
     CDS             complement(3798..4088)
                     /locus_tag="OVW45_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCV5527921.1"
                     /translation="MLPVLWLPSARDDLRQIVAYIAKENPAAARRMKIRIETSVLPLT
                     EHPYLYPPSERVLGLREIVTHPNYIILYRVTASSIEVVNIVHSRRQYPGKNS"
     gene            complement(4088..4282)
                     /locus_tag="OVW45_003563"
     CDS             complement(4088..4282)
                     /locus_tag="OVW45_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCV5527922.1"
                     /translation="MSTVLSPMVSEFETVEQENSYNEWLRAKVAASLADPRPAIPHDE
                     VMAEMEQIIAQIAAATHRGE"
     gene            complement(4302..4802)
                     /locus_tag="OVW45_003564"
     CDS             complement(4302..4802)
                     /locus_tag="OVW45_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCV5527923.1"
                     /translation="MKRKSLSDTDLNAARRLKEIWMAKKSQLGLTQERAAEIMGFSTQ
                     GAVSHYLNGQTPLNLEAVLKFAGLLKVPPESIRPDMVDLLQIARMYCREPQQDNIVAL
                     PADTEQTEDELPFAVEPMERDLIHTFRAFPKEDQEHMLQEMKDKKESMDRTVARWLAA
                     QKGRRA"
     gene            4914..5177
                     /locus_tag="OVW45_003565"
     CDS             4914..5177
                     /locus_tag="OVW45_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCV5527924.1"
                     /translation="MNLGEYLHHFHITQSSFAEIVGASQGMVSHVITGRAKLTGEKIL
                     RWCEATGWVVTPHEIDSKTYPNPTDGLPAEFQANTQPSAGVDS"
     gene            5174..5599
                     /locus_tag="OVW45_003566"
     CDS             5174..5599
                     /locus_tag="OVW45_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCV5527925.1"
                     /translation="MKIKHEHIRMAMKAWAHPDGEKVPAAEITRVYFELGMTFPVLHN
                     DSKHNTLYLNTQKIFRWLDKDTPDAVEKIQALLPAIERAMPPPLVARMRSHSSAYFRE
                     LVETHERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            5671..6735
                     /locus_tag="OVW45_003567"
     CDS             5671..6735
                     /locus_tag="OVW45_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HCV5527926.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDTDKDTDKEDQNTM
                     VHGVKNATNQAGDVQTVNPGQPAGSTPEADSAYALKADSGAVQQVMTARPEQSLQLQQ
                     PEADSAIQREADRVAPENTGQPVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWKAR
                     LREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFENPWLLPVSGTNN
                     QRCVNHISEPDTEIPPGFRG"
     gene            6742..7488
                     /locus_tag="OVW45_003568"
     CDS             6742..7488
                     /locus_tag="OVW45_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCV5527927.1"
                     /translation="MKNIAAAGVLERIHRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNRQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNWLMAKGRSVIIVTVSDVMSVLHDSYDNGKSGEKFLQE
                     LCGVDLLVLDEIGIQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGCRWVTFNWDSWRSNVSFPGVVK"
     gene            7510..8235
                     /locus_tag="OVW45_003569"
     CDS             7510..8235
                     /locus_tag="OVW45_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HCV5527928.1"
                     /translation="METVFDALKALKRASSHVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASIAETDGKVFPQPAGVALPVQEAA
                     TQEEIKTETVADIVQSLPSFTETQADDLVLPSLHMANRELRRAKSHVQKWERVCAALR
                     ELNKHRDIVRQIVDSSGRIVSEK"
     gene            8251..8673
                     /locus_tag="OVW45_003570"
     CDS             8251..8673
                     /locus_tag="OVW45_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCV5527929.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLIS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSVVDPDLIWSLPDG
                     EIRRYDRHQNIICRECRKSEVMQRVPAFYQGDFQEAVL"
     gene            8731..9087
                     /locus_tag="OVW45_003571"
     CDS             8731..9087
                     /locus_tag="OVW45_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5527930.1"
                     /translation="MEIKPEDELSNIVLFPVKEDNPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEERERRKNIEKLIQIE
                     RNAKARIRRVTKYSPE"
     gene            9338..11134
                     /locus_tag="OVW45_003572"
     CDS             9338..11134
                     /locus_tag="OVW45_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCV5527931.1"
                     /translation="MNVQHFDFFTDNEFSNLERYQTCKAMAKGYAGNYKTGQSRLMHA
                     RSYCFSIVAAYWDTLAKKQKSSIKMRAVPHTVYIEDIPCDARELAERTGELIAQFPAE
                     DAGYLIGSIYTVMLPSAYRSELGAYYTPPPLVARLLDLAEKSGVDFSHASVIDPACGG
                     GAFLAPVAIRMLKKDKGSSPEWMLRRISRRLKGIEIDPFAAWMSLVLLESVLMPLCVK
                     VKRRLPEDTIIVADALQQDKFSGYDLVVGNPPYGRVTLDIKTREKYSRSLFGHANLYG
                     LFTDLAVRMVKEKTGVIAFLTPTSFLGGQYFTALRTLLTEKTTPYALDFVTDRDGVFD
                     DVLQETMLTAFKAGEHKVRAQVSSLVPKGLNKAKIEKIGNVDIEKGGATWLLPRVKDD
                     ACFLNVLKQMSTRLADLGYSVSTGQLVWNRFKSQLRTTKGKNSYPLVWAESITSAGFR
                     FSADRKNHVPYIDITPQQGFLVTKSECVLVQRTTSKEQDRRILAAILPQGFIDETGGV
                     VVENHINIVYSNGLFSAVRPDVIDMLLNSHVVDRAFRCISGSVAVSAYELNSIPLPSL
                     EQVMEIQSLIDAGVHRRIIERTIAGFYGVMIA"
     gene            11131..12213
                     /locus_tag="OVW45_003573"
     CDS             11131..12213
                     /locus_tag="OVW45_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HCV5527932.1"
                     /translation="MNLPVVPPLNVIRERLQLIFPEGTENRGYLIREIAAKTIFVMFY
                     AGAVEGLGRWIRPSQVVSMGDSQAALTDDASRESWVKMTFSKQKIRPADAWYAENTRE
                     PIRDETIKNGLIPCNAIVERKGIPTTSSRPRYSLNRSFAALFDAGLEGDSLLSAISSW
                     QENHLNKAALARLRLLKSGAVIAADAVVVTFPNGEKRTLAPGPSSTIAKAVIEVFAPN
                     FLKNPTVLWLSESGNKVVAQDDVLAKALGLNIDASKALPDIILIDLGEDRTGSDMLVV
                     FTEVVATDGPINRERKVTLTHLAIDAGFSEKNLAFLTAFMDRSALPFKKAIPELAWGS
                     YAWFASEPEHLIDLRDGYPVKISQRK"
BASE COUNT         3235 a         2706 c         3162 g         3258 t
ORIGIN      
        1 atcagttgcg ctctgatttg ttccttcatc ttcaaacacg ccctttgtag tcaggtattc
       61 agtaatgtat ttgttcagtg ccacagggtc tttgtgaatg tcgatcggac gttcacggac
      121 aaggccaaaa atagtctggc ggtcgtagcg aagggcatca ggctgtttgc gcattgatgc
      181 cgagatacgc ttccagtctt cgcggtcgtt gtcgataact tcattttttg cccagcgatg
      241 gatgctgccg tcaatgtttc cggcatccac atcaccaggc cagagagcgt aggccagttc
      301 gtcatccagt gttttccatg tctgcttgta ttcgcgatga atggcagcaa tggccgggct
      361 gatttttcct gttgaatttt cagtgtgctg ttgattggct ctggcacggg cgagatcaac
      421 aacagacgtg tattttccgg tttccttgcg ttcaccttcg cgacattttt tccagatgcg
      481 catctctgcc tgaatttctg gccattttgc accaggctta catttatgct taatccaccc
      541 gatggcatgc agtttaatct ccggacacat ggcgttaact tctggcattt tcatcaacgc
      601 ttcaacgata tgtccgtcga atgttgccat gtcttcctgc aacagttcct gcgcgctaat
      661 caccatatca acggtgatgt tttcacatgt gtcgaactta accatgacag cgttctgtac
      721 ttcaggggcc agcttgtcaa aagtgacgtt catcggttcg gattcagtct caaccgggac
      781 aaaggaagca gactcctcat cccagcggtt ttcctgcata tattcagcat cccaggaatc
      841 gagggcaggg cggggtatac caggtttatc ctcgcagaca ataaatttat aagcgcagtc
      901 ctgagcagcc ggataatgtt ccaggaattg ccagtgaaat tttgcgcggg cgcggcgttc
      961 gtcgccggct tcaatggcag tggctacagg tttagcgcct tcttcttttg ttgccttttc
     1021 gtccggaatg gcggcgcaaa taaagacttt actcattttg ttttaacctc attacagatt
     1081 taagggtgaa caaatccctg ccattgctgg catataaaaa tgaaaccgga tatttattac
     1141 gtggctgctt taaagacctg ccgggacttc tttattatcc tggtgaatga ttttatcaac
     1201 cggataacag ttaccgggaa ttttctgttc agctgcagca gccacgcatt ctttcatgga
     1261 gttgtatact ccggtaacca gttcagctgg ttcgcccgta ttaagaataa cggtcagaac
     1321 gagtgcaaat gctgtattca tcgccagcat cctttttgca tcagacgcaa acgagccagc
     1381 attgaaacaa tgcatatttt atttaatgac tcccgttcgt gttttctctt gttaatggca
     1441 tcttcagtaa ataccgtgtt actgatagtg acaccaattt caaaacaacc ttcagtcgta
     1501 ttaacgtttg gtaataacgt ttccattatc gcgtcctcaa caatgaattt tgagtaattg
     1561 ctctacagtc atatttttaa ttgcgccccg gttaacaaga gtccatccct gttcttccag
     1621 ataaaaccgg aaagtctcca tggtacagac ccgtgcgcca tcaggaacgg tttcggtgaa
     1681 tctgatgttg ccgcgttcgt cgaagtgaac aaccagggtg cggccatcgc ctggaattat
     1741 tttgtcagtg ggtggggtgt tattctggcg cagttcggcc tccatgcgat cgaactcagc
     1801 aatgtaggct tctttaaatg cagcggcttt tttgccagtg aatcccatca ccaggaaaac
     1861 gaagccgttt ttggtgattt ggtacatcgg gcgtttttcg cctttggcgt cagtgtaggt
     1921 gacgtcctca aaattgaggg cgttaaattc aggtgagcat tcagtatttg cgatggcacg
     1981 taacacattg tcgtgacgct tgccaaagaa ctctgcgatc gcaatagacg tagtgacagc
     2041 gcgaccattt tcgatggtta cgtcagggtg agaaagggca gggatagtag ccatgatggc
     2101 atcctcgagt gataagttaa ttaactcacc accgaggttt tccacgacca taagggtggt
     2161 gagacgtaca ggggtggaaa taccggtcac tcgagaaccc ggccagtctt gcgactgccc
     2221 tgcacatccc accataattt gaatggagct gtgcattacg cataaaaaaa ccgcttcagc
     2281 gcggctatgc gctcgagtaa cgttcgggtt tccacgcccg gcacccgttt tataaggtgc
     2341 aggtgcacta taattccacc cgttctggtt ttcaatagct acattcaaca ttttctcttt
     2401 cctttcatca ccgaagtgaa ctttgttgat gcggtgcctg gtgcctccag gtgacgttaa
     2461 ccagttaaca attaacgccg gatacaggga aacccataac cacccgatac gggggaacta
     2521 atcgcttttt aactgtgccg cgtgcgctga gccgcattca ccgcatcaca aaattcactt
     2581 taaaaagggc ggacatcagc cagcaatgaa actgatgccg ccaaaggtac caatcaacat
     2641 ggagtgttgt ggcgtggttg tcacttaagc gtatggtcaa cctgacaacc cggtgtcctc
     2701 aacggggaag gaataactcc gccatactta ccgccgcgtc atttcgcgtt gtgtgcctgc
     2761 ttttaaccac gtcaggcgag gtggtatcct tcttatcccg aataaccaag aaggaaatct
     2821 atatgactaa agaagaattt gtctcttata tttttgataa aacggttgaa atgtatgccg
     2881 ctacttacgg gtcctgtaat cctctgaata aaccagaggg aaaagatgat ttcgacaaaa
     2941 tttaccgctt cttggaggac cgctatatca aaagcttaga ggacgcaggg atcaaatccc
     3001 cagtgaagtc accattgtcc tgagaacttg cagaacgtca tgatcataac ttccatccaa
     3061 accgcgacgg cgaattgctt ctcgtattac cggaagcagt tcgctggaaa tctcggtgca
     3121 tatttcacct gataatacgc ttggctcaag tgagaatatt ggtgaactta cggtcttggt
     3181 ctcgacagtt tcagagtcag tgccaacatt ataaagctca acgaaagcgg tcttgatttt
     3241 ccgggccaga tcttttgctg gctcgcttgc aatatctttc ccgatttctc gcagcacaga
     3301 atgcaatgta tgagctgctg ttttctgtac atcagacggt aaatctttaa attccatcgt
     3361 cagcctcatc agtcagtgtt tctggctaac cagcaacgcg cgccagattc ggttttaaac
     3421 gttttgcttt tggtatacgt catggcggta aacgttccat cctggttagg gaacacgccg
     3481 cacaccagag attcgttgtt gccaagatcg atagtatcca tgctgacctc atttcccctt
     3541 aacgccgggg tggcggaaca aaaaacctgc tgcatagtta aagattgaac cctgccgtca
     3601 tgttcttacg cctcgggctg gctacttaac ccctgaccac tgtctggtaa ctcgaagtat
     3661 tgccctgcat tctgtggggc ggggtgggtt gacgcctgaa acaatagcat cattattttt
     3721 tgatgtaaat agcatcgcta ttgtttttgt tggggcaaga aaaaaccacc ctaaggtggt
     3781 tctgtcggcg ggaataatta actgtttttg cctggatact gtcttcgaga atgcacaata
     3841 tttactactt ctatgcttga tgctgttact cggtaaagga tgatgtagtt aggatgagtt
     3901 acaatttcac gaagaccaag aactcgctcg cttggaggat acaggtaagg atgctctgtg
     3961 agcggcaaaa cggatgtttc aattcgaatt ttcattctgc gtgctgccgc gggattttct
     4021 ttagctatgt aggctacgat ctggcgcaaa tcatcacgag cagatggtag ccataaaacg
     4081 ggtaacatta ttcacctctg tgagttgcag cagcaatttg ggcaataatt tgttccattt
     4141 ccgccattac ctcatcatgc ggaatcgccg ggcggggatc cgcgaggctt gctgctactt
     4201 tggcccgcag ccattcgttg taactatttt cttgctcaac tgtctcaaat tctgaaacca
     4261 ttggggaaag gactgtactc attttacata tctcctctga tttaggcgcg acggcctttt
     4321 tgtgcagcta accaccgcgc aaccgtacga tccattgatt cttttttatc tttcatctcc
     4381 tgaagcatgt gttcttgatc ttctttcgga aaagctctga acgtatggat taaatctcgt
     4441 tccatcggct ctacggcaaa cggtagttca tcctctgttt gttctgtgtc tgctggtaat
     4501 gcgactatgt tatcttgctg tggttctcga caatacatcc ttgcaatttg cagcaaatct
     4561 accatgtctg gcctgattga ctcaggggga actttcagta agccagcaaa ctttagaaca
     4621 gcctcaaggt ttagtggggt ttgaccattt aggtagtgac ttaccgcccc ttgtgttgaa
     4681 aaccccataa tttccgccgc acgttcctga gtcaatccca actgagattt ctttgccatc
     4741 cagatttctt tcagtcttct ggcggcgttg aggtcggtgt ctgacaatga ttttcttttc
     4801 atacatccaa ttctaataag attgctaatc actttgaaat agcatcacta tttactttta
     4861 aaaataacag tgctattaat tatgcattgg taacacatca catagggcgg attatgaatc
     4921 ttggagaata tttgcatcat tttcatataa cccagagttc atttgctgag attgttggcg
     4981 catcccaagg gatggtcagc cacgttataa cggggcgtgc aaaacttaca ggagaaaaaa
     5041 tattacgttg gtgtgaagca acgggatggg tggtgacccc acatgagatt gacagtaaaa
     5101 cctaccccaa cccaactgac ggcttgcctg ctgagtttca ggctaacaca caaccatcgg
     5161 cgggggttga ttcatgaaaa tcaagcatga acacatccgc atggcgatga aagcctgggc
     5221 gcatccggac ggtgaaaaag ttccggcagc tgaaataacc agggtttatt tcgaactggg
     5281 aatgacgttt ccggttctgc ataacgacag caagcacaac acgctttatc tcaacaccca
     5341 aaagattttc cgttggctgg ataaagacac ccctgacgct gttgaaaaaa ttcaggcgtt
     5401 gttacctgcg atcgaaaggg caatgccacc tccgctggtg gctcgaatgc gcagccacag
     5461 ttcagcttat tttcgggaac tggtggagac gcatgaacga ctggtgagag acgctgatga
     5521 ttttgtcgca gtggcaatcg ccggtttcaa tcagatgaac cgtggtggcc cggcaggaaa
     5581 tgctgtggca gtacattgac tgacaatagc catatcgaat cgctcccggc aactcgtgag
     5641 taaaaagatt cggtatcaga agaggtgagt atggctaacg cctggctcag attatggcat
     5701 gacatgccaa atgaccctaa gtggcgaaca attgccaggg tgtcagggca gccaattgca
     5761 acagtgatgg cagtgtatat ccacctcctg gtgagcgcgt cacgaaatgt cacgcgaggt
     5821 cacattgatg tcacgacaga agatttggca agtgcgctcg acgtgacaga agaggtaatt
     5881 gattcaattt tgcagacgat gcaggggcgg gtacttgatg gtgatttaat cactggatgg
     5941 gaaaaacgcc aggtgcttaa agaggacaac ggcaatattt cgcaaaccgc aaaatctcct
     6001 gcagagcgca agagggcgca gcgagagagg gaaagaaagc gggaacaaaa tggcgattgt
     6061 cacggcgcgt cacgaaatgt cacgcacatg tcacgacgag tcacgacaga tacagataaa
     6121 gatacagata aagaagatca aaacactatg gtccatggcg taaaaaacgc cacgaaccag
     6181 gcaggggatg ttcagaccgt caatcctggt cagccagcag gctcgacacc ggaagccgat
     6241 tcagcgtatg cgctgaaagc cgattcgggc gctgtgcagc aggtgatgac cgcaaggccg
     6301 gagcaatcac tccaactgca gcagcctgaa gccgattccg ccattcagcg ggaagccgat
     6361 cgggtagccc cggaaaacac cgggcagcct gtgggacgag tggattatcc ggatgtgttc
     6421 gaacaggtct ggcgggaata cccgttgcgt gccggggcaa acccgaagaa atccgcattc
     6481 agtgcctgga aggccagatt gcgcgagggg gtgccaccag agaccatgct ggatggtgtg
     6541 aggcgttacg cgagatacct ggcggcgacc gggaaagcgg gaacggaatt tgttcagcga
     6601 gcgacgacgt tttttgggcc ggaccggaat tttgaaaacc cctggttgct cccggtaagc
     6661 ggcacgaaca accagcgttg tgtgaatcat atttctgaac cggataccga aattccgccg
     6721 ggattcaggg ggtgatgtgg catgaaaaac attgcggcag ccggggttct tgaacgtatt
     6781 cacagacttg caccacaggg ggcggttcca ccgtaccgga cggtggagga gtggcgggaa
     6841 tggcaacttg ctgaaggacg aaaacgcagc gaggagatta accgccagaa tcgccagttg
     6901 cgggtggaaa aaatcctgaa tcgttcgggc atccagcctc tgcacagcaa atgctcgttt
     6961 gcgaattatc aggtgcagaa cgacgggcaa aaatacgcgc tgagccaggc caaatccata
     7021 gctgacgaac tgatgaccgg gtgcacgaat tttgtgttca gcggtaaaac cggcaccggg
     7081 aaaaatcacc ttgcagcggc gatgggtaac tggctgatgg cgaaggggcg cagcgtgatt
     7141 atcgtcaccg tgtctgatgt catgagcgtg ttgcatgaca gctacgacaa cggcaaatcc
     7201 ggtgaaaaat ttttacagga gctttgcggg gttgatttgc tggtcctgga tgaaataggc
     7261 attcagcggg agacgaaaaa cgagcaggtg gtattgcacc agataattga tcgccggaca
     7321 gcatcactgt gcagtgtcgg gatgttaaca aacctgaatc atgccgcaat gagcacgctt
     7381 cttggtgaga ggattatgga ccgcatgacc atgaacggtt gtcggtgggt gacgtttaac
     7441 tgggatagct ggcgttcaaa tgtcagcttt ccgggagttg tgaagtaatt tttgttggag
     7501 gatgttttaa tggaaactgt atttgacgca ctgaaagcac tgaaaagagc ctcttcacat
     7561 gtggtggcgt cccgccttgg aatcagccgt gaagatgcgg tcaacgaact gtggaaactg
     7621 aagcgccgtg gtgaagcgga taacaagggt tcgatgtggt ggctgattca ggctggtgaa
     7681 agtgaaccag tgtcaccggt accgaaagtg acagcgcaaa tgctgactga ggcgattgaa
     7741 caacatggcc cacaaacggc ggatgagctg gcactgatgt tcgggattac ctcccgccgg
     7801 gcgaattcat cgctggccat ggcaatcagc aaagggcgtc tgattcgcgt gaatcagggc
     7861 ggtaaatttc ggtactgcat accgggcgct gatttaccgg cagagccgaa agccgcatcc
     7921 atagcggaaa cggatggtaa agtctttcct cagccagcag gtgttgcgtt accggtacag
     7981 gaagctgcaa cacaggaaga aattaaaaca gaaactgtgg cggacattgt gcagtcgttg
     8041 ccatcgttca ccgaaacgca agcggatgac ctggttttac catcgctgca tatggcaaac
     8101 cgcgaactgc gtcgggcgaa aagtcatgtc cagaagtggg agcgtgtctg cgccgcgttg
     8161 cgggagctga acaagcaccg ggatattgtt cgacagattg tcgattcctc cggtcgtatt
     8221 gtgtcggaaa agtgattgcc gggggcgctt atggcaaaag tatttacaca ggaagagcgg
     8281 gaaaaaatta aggggcaggt tgttgaactc gtacgccaga gtgggcgcga gacgttacga
     8341 caactggaag ctaaaaccgg ggcgacaaga tatttaataa gcgttctcgc cagagagctg
     8401 gttgccagtg gggatgtata caactctggc tacgggttat tcccgtctga acaggctcgt
     8461 aaggactggc aaaacgcccg caaaaaactc tcgagggcaa agctgaagaa accatctgtg
     8521 gttgatccgg accttatctg gtcattacca gacggagaaa tacgccgcta cgacagacat
     8581 cagaacataa tctgtcgcga gtgccggaag agtgaagtta tgcagcgtgt accggctttc
     8641 tatcagggtg attttcagga ggcggtactg tgagtgaaat tagctatcag gcttcaatta
     8701 ccgctggcat tcgcatcaaa ggagaggagc atggaaataa aaccagaaga tgagttaagc
     8761 aatattgttt tatttccggt aaaagaggat aaccctcgta atcaggttaa ttttctttat
     8821 gagccatcgg aaagaccata ttgccatcac gcttctgtcc gggttgacga aaaagagcgt
     8881 caggtccgct gtaaaatttg cggtgcagtt gtggagccat ttgactggat gctctctgtg
     8941 gcgaaaagag aaaccagact ggcagatgat gtaaggctct tgcgccagga ggagcgggaa
     9001 aggcgaaaaa atatagaaaa gctaatccag attgagcgta atgcgaaagc gcggatacgc
     9061 agggtgacaa aatatagccc cgaataatca aggttatttc tggaagtcaa gtcgtgagtg
     9121 atgaggacta acaaatacgg tgaactcgat aaagagattc aggacgaact ggaggccagg
     9181 aatgattgac aaatcaccaa gtgagttcag ctacaaagat ttattgtcat aacgggttat
     9241 gagatcgact tggtgcgtga tattcttcgg gccagtttgc tatgcgccac catgagatgt
     9301 tatggcccgc atgtttgata cattaggatt ttacattatg aatgtgcaac attttgattt
     9361 ttttacagat aatgagtttt ctaatctgga aagatatcaa acttgtaaag ccatggccaa
     9421 aggttatgcg ggcaattaca aaaccgggca gtcaaggttg atgcatgccc gctcatactg
     9481 cttcagcatc gtagctgcat attgggatac ccttgcaaaa aagcaaaaaa gcagcattaa
     9541 aatgagggca gtaccgcaca ctgtttacat agaagacatt ccatgtgatg cacgcgagct
     9601 cgctgaacga acaggtgagc ttattgctca atttccggcg gaggacgctg gatatctgat
     9661 tgggtcaatt tatacggtaa tgctgccatc cgcttaccgt tcggaattgg gggcttacta
     9721 taccccccca cctttggtag cgcgtctctt ggacctggct gaaaagtctg gtgttgattt
     9781 ttctcatgca tcggttattg atcctgcgtg tggtggcggt gcatttttgg caccagtagc
     9841 cattcgaatg ttaaaaaaag acaaggggtc ttcccctgaa tggatgttga ggagaatcag
     9901 tcgtcgttta aaggggatag aaatcgatcc atttgcggct tggatgagtt tggtattgct
     9961 cgaatctgta ctaatgccac tatgtgtaaa ggtaaaacgt agattgcctg aagatacaat
    10021 tattgttgct gatgcgcttc agcaagataa attttctggt tatgatcttg tggtagggaa
    10081 tccgccttac ggacgtgtca ctctcgatat taaaacgaga gaaaaatatt ctcgttcgtt
    10141 atttggtcat gcaaatctat atggattgtt caccgactta gctgttcgca tggttaaaga
    10201 gaaaacagga gttattgcat ttcttacgcc aacttctttt cttggtgggc agtatttcac
    10261 cgcgctgcga acccttttga ccgaaaaaac tactccgtat gcccttgatt tcgtcactga
    10321 cagggatggg gtctttgacg atgttctcca ggaaacaatg ttgacagcat tcaaggcggg
    10381 agaacataaa gttcgggccc aggtatcctc acttgtccct aaggggttga acaaagcaaa
    10441 aattgagaaa ataggtaatg ttgatataga aaaaggtgga gcaacgtggc ttttgccacg
    10501 cgttaaagat gatgcctgtt ttcttaatgt attaaaacag atgtctacgc gtttagctga
    10561 tctgggatac tcagtctcta caggtcagtt agtctggaat cgatttaagt cgcagttgcg
    10621 tacgacgaaa ggaaaaaata gctacccatt agtctgggcg gaatcaataa cttcagctgg
    10681 ttttcgtttt agtgcagaca gaaaaaatca tgttccctat atcgatataa ccccacaaca
    10741 gggtttcctt gtgacaaaat ctgaatgtgt gcttgttcag cggacaacat cgaaggaaca
    10801 ggacagaagg attttagcgg caattttacc tcagggattt atagatgaaa ctggtggcgt
    10861 agtagttgaa aaccatatta atattgtata ttcaaacgga ttgttctctg cggttcgccc
    10921 tgatgttatt gatatgctac ttaattcgca tgttgtcgat cgggcgttca ggtgtataag
    10981 cggaagtgtt gctgtttcgg cctatgaatt gaattcaatc ccgttgcctt ctctggagca
    11041 ggtgatggag attcaatcgt taattgatgc tggcgtccac agacggatta ttgagagaac
    11101 tatcgctggt ttctatggag tgatgattgc atgaatctgc ctgttgttcc cccattgaat
    11161 gttatccggg aaagattaca attaattttc cctgaaggga ctgagaatcg tggatattta
    11221 attcgggaaa tagctgcaaa aacaattttt gtcatgtttt atgctggtgc ggttgagggg
    11281 ctggggcgat ggataaggcc cagccaggtt gtaagtatgg gagattcgca ggctgctctt
    11341 actgatgatg cgtccagaga aagctgggta aagatgactt tctctaaaca aaagataagg
    11401 cctgctgatg catggtatgc ggaaaatacg cgtgagccaa tacgtgatga aactataaaa
    11461 aatggcctaa taccttgcaa tgcaattgtt gagcgtaaag gtatacccac cacatcctct
    11521 cgtcctcgtt acagtttgaa tcgctctttt gcagcactct ttgatgccgg gcttgaagga
    11581 gattcgctgc tgagtgcaat aagttcctgg caagaaaatc atttgaacaa agccgctctt
    11641 gctaggttgc gtttattgaa atcaggggca gtgattgctg ctgatgccgt agtcgtcact
    11701 ttcccaaatg gagagaagag aactttagct ccgggcccgt caagtacaat agctaaggcc
    11761 gtcatcgaag tttttgctcc taattttctt aagaatccaa ctgtgctctg gttgtctgaa
    11821 tccgggaata aagttgttgc tcaggatgat gtattggcaa aagcattggg gctgaatatt
    11881 gatgcctcaa aagcacttcc agatattatt ttgattgatt tgggagagga ccgaacgggt
    11941 tcggacatgc ttgtagtttt cactgaagta gttgcgacag atgggcctat aaacagggaa
    12001 cgaaaagtta ctctcactca tttggctatt gacgctgggt tcagcgagaa aaatcttgca
    12061 ttccttacgg catttatgga cagaagtgct ctgcctttta aaaaggcaat accagaattg
    12121 gcgtggggat cttatgcatg gtttgcttct gaaccggagc acttaattga tcttcgggat
    12181 ggttatccgg taaagatcag ccagagaaaa tgaccaaagt ttgatatccg ccacgccctg
    12241 aaggatgggg gttttacggt gcactggata aaaaatcggt ttcatagatt tgcccaccgt
    12301 ggtatacgcc tgcgataaaa actttattgt catcaacggc aaaagcaata attgttctgt
    12361 g
//
LOCUS       DAKISA010000120        12340 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.120, whole genome shotgun sequence.
ACCESSION   DAKISA010000120 DAKISA010000000
VERSION     DAKISA010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12340
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.120"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..178)
                     /locus_tag="OVW45_003574"
     CDS             complement(<1..178)
                     /locus_tag="OVW45_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCV5527933.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQSEYDSQWATICSIAPKIGCTP
                     ETLRVRVRQHERDTG"
     gene            276..536
                     /locus_tag="OVW45_003575"
     CDS             276..536
                     /locus_tag="OVW45_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5527934.1"
                     /translation="MDQEMTFSLSYEQLTRFAEKRIRECNLDSHGVTYLCESAKAGAV
                     LIFWHELAINGYTSMNAIKRQEIIDADHQRLRKLIWPEDDWK"
     gene            533..1123
                     /locus_tag="OVW45_003576"
     CDS             533..1123
                     /locus_tag="OVW45_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5527935.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLEQSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRIRAEQR
                     AAEVVVLPLVPAAGLPAGGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     WVVRREGDPLEVAQWLQACHDAGMEVRVQINESAAP"
     gene            complement(1143..1490)
                     /locus_tag="OVW45_003577"
     CDS             complement(1143..1490)
                     /locus_tag="OVW45_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5527936.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLSGLLMASFHWW
                     RRKVNRLPPWDDV"
     gene            1619..1954
                     /locus_tag="OVW45_003578"
     CDS             1619..1954
                     /locus_tag="OVW45_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin 1A immunity protein"
                     /protein_id="HCV5527937.1"
                     /translation="MNRKYYFNNMWWGWVTGGYMLYMSWDYDFKYRLLFWCISLCGMV
                     LYPVAKWYIEDTALKFTRPDFWNSGFFADTPGKMGLLAVYTGTVFILSLPLSMIYILS
                     VIIKRLSVR"
     gene            3473..4159
                     /locus_tag="OVW45_003579"
                     /pseudo
     CDS             3473..4159
                     /locus_tag="OVW45_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554952.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            4492..5367
                     /locus_tag="OVW45_003580"
     CDS             4492..5367
                     /locus_tag="OVW45_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HCV5527938.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLTDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSEQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIISMHEGNLESQQLTSMLSIQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKSMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            5375..6151
                     /locus_tag="OVW45_003581"
     CDS             5375..6151
                     /locus_tag="OVW45_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HCV5527939.1"
                     /translation="MMNILHFSQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
     gene            6320..8581
                     /locus_tag="OVW45_003582"
     CDS             6320..8581
                     /locus_tag="OVW45_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCV5527940.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNDLEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDD
                     KEGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSMPEVIGNDW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTY
                     FGAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDETHPANSA
                     RGRTWIFGGDIRF"
     gene            8650..9825
                     /gene="senB"
                     /locus_tag="OVW45_003583"
     CDS             8650..9825
                     /gene="senB"
                     /locus_tag="OVW45_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HCV5527941.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHFRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(10351..10812)
                     /locus_tag="OVW45_003584"
                     /pseudo
     CDS             complement(10351..10812)
                     /locus_tag="OVW45_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            complement(10850..>12340)
                     /locus_tag="OVW45_003585"
     CDS             complement(10850..>12340)
                     /locus_tag="OVW45_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCV5527942.1"
                     /translation="AEQEALIHALQEKLSNREREIDHLQAQLDKLRRMNFGSRSEKVS
                     RRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPFPESLPRDEKRLLPAAP
                     CCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQCDAIVQAPAPSRPIERG
                     IAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELSRSLLSGWVDACCRLLSPMEEAL
                     HGYVLTDGKLHVDDTPVPVLLPGNKKTKTGRLWAYVRDDRNAGSTLAPAVWFAYSPDR
                     KGIHPQTHLAGFSGVLQADAYAGFNELYRDGRITEAACWAHARRKIHDVHVRTPSALT
                     EEALKRIGELYAIEAEIRGMTAEQRLAERQLKTKPLLKSLESWLREKMKTLSRHSELA
                     KAFAYALNQWPALTYYADDGWAEADNNIAENALRMVSLGRKNYLFFGSDHGGERGALL
                     YSLIGTCKLNGVEPESYLRYVLDVIADWPINRVGELLPWRVALPTE"
BASE COUNT         3270 a         2926 c         2980 g         3164 t
ORIGIN      
        1 ccccggtatc ccgctcatgc tggcgaaccc ggacacgcag agtctccggc gtacagccaa
       61 tctttggagc aatggaacaa attgtcgccc attgtgagtc atattcgctc tgactttcca
      121 gaaccatacg gactgcccgt tgacggactt cgggggaaaa acgagtattt ttagtcatcc
      181 tgtttacctc tttctcagga agtttagtct ccaggattcc cggggcggtt caatgatttt
      241 tacctgtcag aacattatca gatggaacgt gcaggatgga tcaggaaatg acgttttcac
      301 tcagctatga acaactgacc cggtttgcgg aaaaacgcat ccgggaatgc aatctggaca
      361 gccacggcgt aacttatctc tgcgagtcag ccaaagccgg agccgtgctg atcttctggc
      421 atgaactggc cataaatggc tatacctcaa tgaatgccat aaagagacag gagatcattg
      481 acgccgacca tcagcgcctc agaaagctca tctggccgga ggatgactgg aaatgactca
      541 gagccgccga ccgtcgccac tgcagcggag agtgctgatt gtgctggctg cccttgatga
      601 aaaacggcca ggacccgtgc tgacgcggga catagaacgg gtgctggagc aaagcgggga
      661 agccccggtc tacgggccaa acctgcgcgc ctcctgccgt cgtctggagg atgccggctg
      721 gctgcgtacc ctgcgcgctc cgaacctgca gctggcagtg gagctgacgg acgccggccg
      781 ggccgtggca cagcctctgc tgctggcaga acaggaccgc atccgggcag aacagcgtgc
      841 ggcagaagtc gtcgtactgc cgctggtccc ggcagcaggt ctgcccgcag gcggcacttc
      901 tgccactgat ctggcggtgg agctgaacgg cataacgtat caggcctgcc gtggggattt
      961 tgtggtgcgt ctcgacggca gcacctgtct gcagttatgg aataaagaag gctgggtggt
     1021 acgccgggag ggcgatccgc tggaagtggc gcagtggctg caggcctgtc acgatgcagg
     1081 aatggaggtt cgcgtacaga taaatgaaag tgccgctccg taagaacacg cctgtgcacg
     1141 gtttacacat catcccatgg tggcagtcgg ttaactttcc tgcgccacca gtggaaagag
     1201 gccatgagca gtccggacaa aaaacccccc gtgatactta taataatggc ctcacctgcc
     1261 accattcctg acggccccca gtagataaac cacattgcac atccccagga aatgccccat
     1321 aagccccccg tcagcaccgt gacctgccag aacggcataa agggtaatgg cggaagccgg
     1381 atacccagcc gccagagaat acgcagaaga ggaggggcat aattgctccg ccacatcttt
     1441 ttgctgtcca tcagggcaat ggcccgggct tttttctgct caaaagtcac aggagcacct
     1501 ccgttccatg atgtaaacgt gacacagctt attgttattg attattatta tcatgctgtg
     1561 gttatgttgt catgcatcag tacactgaca aaagtgcaaa agaaaggaaa actggcagat
     1621 gaacagaaaa tattatttta ataatatgtg gtggggatgg gtgacgggag gatatatgct
     1681 gtatatgtca tgggattatg attttaaata cagattactg ttctggtgta tttctctctg
     1741 cggaatggtt ttgtatccgg ttgcaaaatg gtatattgaa gatacagctc tgaaatttac
     1801 ccggcctgat ttctggaaca gcggtttttt tgctgataca cctggaaaaa tgggattgct
     1861 tgctgtttat acgggtactg ttttcatatt atctcttccg ttaagtatga tatatattct
     1921 ttctgttatt ataaaaaggc tgtctgtaag atagacagcc tgtcttatat tttatttaaa
     1981 taccccagaa cttatgccac aagaattatc taaaagttgc cgttctactc cgttagtttg
     2041 tagttctatc atagtcttat taactcgatc acgaggtctg tatgacgggg aagcttgcgc
     2101 tgtaagatta tatgataatg cacatattgt aatgatagtt gtttttatct ttgtgtttaa
     2161 tgtgaaaatt ttataaagca tataatgttc cctattttta ttttgtttat aatttattgg
     2221 ttgtatgtta aatctatcat aagtctaatg attaaaatgg ttagtggtgt caggattagt
     2281 gaaaataaag gatgtaatat gctattcttt ctctgtaagt acttgagaat actaaaccat
     2341 agtacaagaa cgattatgta catgtagtga gaattggaaa acatagtttc ttcccgtgtt
     2401 aatgagagta tgtgttttta tgtggggata tgtacacccc atatagatgt attatgttat
     2461 atttataata tacctcggca aaataaaagg attgttatag aaatagggaa ggctataaaa
     2521 aagcttatat taaggtctgt tttcttctta gaaaaataat tctttgttct aattattata
     2581 gatgtaatcc cccaaacaat aaaaagaatg gagctgcttg taattatttg atatatcaaa
     2641 attgttttcc tctatttttg tgtagataga tgttaatggc actgggtgca atcaattaag
     2701 aaaattgacg ccagactaat tattaataat ccagttatct ttttattcat atcatctcct
     2761 ttccattggt taatttatcg gaaattcatc cccactaact aaaacgatcc acacaataaa
     2821 actaaatcta attgcaggca atcaattcca gtcgatatat catctgtatg gaaatcatcg
     2881 ctcatcactc atcgcagcct gaaagcccag agcaacgggg caggtctgca tataagtggt
     2941 tacacggcag aattccagag ggttctgtca ctaaattcag tcactgacaa atttgcatga
     3001 tgttgaatag acgagatttt gtgtcgaaat agttgtttca gtaactgtca ctcaatcggt
     3061 ggtttatgtg actatattcg caaaaactgg tcgcagaaaa gtcctgtttt cagcttggaa
     3121 acccggtaat cagaataatg gataggggaa caaaaatctc tgtatagaac gctccattga
     3181 gggagagaaa acgtgtcatt ttgacccgtt cggtattcag aacacacaaa accgatcgcg
     3241 ggtcgctttc gtgcatagtt atgcaacgcg ctaaaaacga ttctgacgca ttttttatgt
     3301 ttaccctgtt ttttcttgtc tttttgagtt ttttgcgtca gagagcgtct gagagggcac
     3361 gtacaacggg agttatggta aatggatcgg gttttcggga aggttcgaca ggatttgccg
     3421 ttgggtgtag tgtaagcgac tgaaaaacaa acgcccctga aatcatgtcc agatccggca
     3481 gaaccagcct catgcgcagt tctggctggt ttttgacgtt gatcgcgagg gggcagcgat
     3541 tgactggagc gaccggaacg ctcccgcgcc gaacatcacc gtaaaaaatc ctgtgaacgg
     3601 tcatgctcac ctgctctacg cgctcaatat cgccgtgaga accgcgccgg acagctcggt
     3661 taaggcgctg aaatacgccg ctgcgattga acgttcgctg tgtgagaaac tttgtgctga
     3721 tgtgaactac agcggactga tttgtaaaaa tccgttccac ctggaatggc aggtgatgga
     3781 gtggcgagag gaagcctata ccctcgatga actggctgat tatctcgatt tgagcgcctc
     3841 agcgcgccgt agcatcgata aacattacgg gatggggcga aactgccacc tgttcgaaat
     3901 gacgcgcaaa tgggcttaca gggcgattcg tcagggctgg cccgaattct cacagtggct
     3961 tgatgccgtg attcagcgtg tcgaaatgta caacgcatcg cttcccgttc cgctttcacc
     4021 gcctgaatgt cgggctattg gcaagagcat tgcgaaatac acgcacagga acttcacgcc
     4081 ggaaactttc gcacagtatg tggctgatac gcacacatta acgtacgttt tcgttccatt
     4141 ggccctcacc ccccgataac gacccaggta gcgcgccgta ttccccccgc cggccgtttt
     4201 cttcgacatg tacatcaggc gcggaactca tcggggcgcg ggaatgaaat cccattcaga
     4261 ctccggatag attcaagcaa gcccacttat cgtcaaaatc ggtgttgcaa aaacgggagt
     4321 gaccatagat ccgttttcca agcggacccc ataatatcgg ctggtaaaca tcactcgcta
     4381 gtgcattctt tttgttggta atgataatca tttacattca ttctttgatg ttttatcata
     4441 attaatgttt tgtcaataaa tatatggaaa catattgttt cagatgggaa tatgagaaag
     4501 ttcatcctta tttctatgat aacgttagct agtgttagtc tgggagcctg cagacagaat
     4561 gtcaccataa agcaggatgc gccagggcag aaagctttcc tgactgacgg gcagatttac
     4621 gatttgcaca gtggcaaaat tatttctagc agcgagcttt tggcggatct tgctaccgct
     4681 cgacatctta ttattggtga aaaacacgat aatgccgagc atcatcagat tgagctttgg
     4741 cttattcaaa atttgctcat ccagcgacca caggggagtg tgttgcttga gatgttgacg
     4801 tcagagcaac agccccgagt taaccaagtt aaatgttggc tgaaagataa tcctgtggtt
     4861 cgagatagcc gtgttcagga actacttaac tggcaaaaag ggtggtcctg ggagatgtat
     4921 ggcgacatcg tcatgcagct attacgtggg ccttacccgc tgctcaatgc caacattggc
     4981 cgggagcaga tgttggcact gtacaaaaaa aacgaatttc ctaaaggaaa aaaatccact
     5041 gcacctgtgg tacaagaagc tctgcgtgaa accatcatat ctatgcatga aggcaacctt
     5101 gaaagccagc aactgacgtc aatgctctct attcagcagc agcgggatcg ctacatggcc
     5161 cgccagcttc tgagtgcccc ggtaccgtcg ttgttgattg caggtggtta tcacgcgtca
     5221 aaaagcatgg gcgtaccgtt acacatggaa gatcttgcca ccggcaccca tccagtggtg
     5281 ttgatgcttg cagaaaaagg aatgaacatc acagtagatc atgctgacta cgtgtggttt
     5341 gtagcgccag atacaacaaa aaggtaaacc atggatgatg aatattctcc acttctcaca
     5401 gtcggtaaaa tggagcagct ggtttatctg ttctctactg cttcatggac tgattttcct
     5461 tgcgtttatc tggcgttttt ccgaagttca gcccgctatg tctcctgcac cggcgatcat
     5521 gctgcaatgg gcagaaatta tagaggctcc ttcttctcca ctttcgcttc cggtgggaat
     5581 cgctcaacag gagtccgcag tgacagaaga aaagcaacaa actgaggaca gacaacaacg
     5641 tcctgtcact gaagacagtg atgcaaccat tgaaataact aggaagaaaa agtcatccga
     5701 tggagaaaag aaaaagacac gtccacctcg taagataaag gctcaaacca gcgacagcaa
     5761 ccccacagcc gtttccagta atgctgcacc tcaggctctg gtcgaatcgt cccgtattgc
     5821 tgcgcctttt aacagtgact caacaaagcg agataattct gaggcatctt gggaaagcag
     5881 agtaaaaggc cacctaaacc gttataaacg ttaccctggc gatgccagaa aacgtgcacg
     5941 aaccggcaca gccgtcgtga cttttaccgt aaacacggaa ggcactattg tcagtagttt
     6001 cttggaaatc tcttcaggaa ccctttctct tgaccgcgaa gctattgctg ttttagaacg
     6061 cgctcaacca cttcccaaac caccgcctga aatattggag ggaggattat ttaaagtaaa
     6121 aatgccgatt acctttaagt tgaaggagta aagggataat tggccgttca agttcattta
     6181 actgaaattt aattttcatt tttatgctga acattttatc aaacttttcc actgtcgaaa
     6241 aaatcaaaaa gacagtagaa cataattaat aaacaaatag ttatacttat ctttattaat
     6301 attataccca aggaaattta tgaacgttat aaaactggct atcggctcag gcatattatt
     6361 gctcagctgc ggtgcttact cacaatccat cagtgaaaaa actaattccg acaaaaaagg
     6421 agcggcagaa ttcagtccgc tcagcgtttc tgtcgggaag acgaccagtg agcaggaagc
     6481 tctcgagaaa acaggcgcga ccagttcccg gacaacggac aaaaacctgc aatcacttga
     6541 cgcaacagtg cgtagtatgc ctggtactta tactcaaata gatcctggtc agggagcaat
     6601 cagtgtgaat attcgaggca tgagcggatt tggtcgtgta aacactatgg tcgatggtat
     6661 tacccagagt ttttacggaa cctctacctc cggaacaacg acgcatggtt caactaacaa
     6721 tatggctggc gtacttatag atcctaactt actggtagca gttgatgtta cacgcggtga
     6781 cagcagtggc tctgaaggga tcaacgccct tgccggtagt gcaaatatgc gtactattgg
     6841 cgttgacgat gtaatattta acggtaatac atatggcctt cgttcacgtt tctctgtcgg
     6901 tagtaatggg ctgggacgca gcggaatgat cgcccttggt ggaaaaagcg acgcttttac
     6961 ggatacggga agcattggcg ttatggctgc tgtgagcggc agttctgtgt actctaattt
     7021 ctcaaatggt tctggaatta acagcaaaga gtttggttat gataaatata tgaagcagaa
     7081 ccccaaatcc caactgtata aaatggatat cagaccagac gaatttaaca gcttcgaact
     7141 ttccgctcga acctatgaaa ataaatttac acgtcgtgat ataaccagtg acgactatta
     7201 cattaaatat cattacaccc ctttttctga attaattgac tttaacgtaa cggccagtac
     7261 cagtcgcggt aatcaaaagt atcgtgatgg ctcgctgtat actttctaca aaacctcagc
     7321 gcaaaatcgt tctgacgcgc tggatatcaa caataccagc cggttcactg tcgcggacaa
     7381 tgacctggag tttatgctgg gcagcaaact gatgcgtacc cgctatgacc ggaccattca
     7441 ctcagcggcg ggcgacccga aagcgaatca ggaatcgatc gagaacaatc cgttcgcacc
     7501 ctccggccag caggatattt cagcgctgta taccgggctg aaggttacgc gcggcatctg
     7561 ggaggcagat ttcaatctca actacacacg taacaggatc acagggtaca agcccgcctg
     7621 cgattcacgc gttatctgcg tgccacaggg tagctacgat attgacgata aagagggtgg
     7681 cttcaaccct tcagttcagc tttctgctca ggtaacacca tggcttcagc cgttcattgg
     7741 ctacagcaaa tccatgcgcg ccccgaacat ccaggagatg ttcttctcta attcaggagg
     7801 cgcatccatg aacccattcc tgaagcctga acgtgcagaa acctggcagg cgggttttaa
     7861 cattgatacc agagatttac tggtcgaaca ggatgccctg cgctttaagg ctctggcgta
     7921 ccgcagcagg atccagaact acatctacag cgagtcttat ctggtttgtt ctggaggtcg
     7981 taaatgcagt atgcctgagg tgattggcaa tgactgggag ggcattagcg atgaatacag
     8041 cgacaatatg tacatctacg ttaactcggc aagcgacgtt atcgcaaagg gcttcgaact
     8101 ggagatggat tatgatgcag gttttgcttt tggccgactc tctttcagcc agcagcaaac
     8161 agaccagcca acctccatcg ccagcaccta ctttggcgca ggggatatga ccgaactgcc
     8221 cagaaaatac atgacgctgg atactggtgt tcgcttcttc gataacgcgt tgaccctggg
     8281 cactatcata aaatacacag gcaaggctcg tcgcctgtcg cctgattttg agcaggacga
     8341 acataccggc gcaataatca aacaggattt gccgcagatc ccaacgatta tcgatctcta
     8401 tggtacttac gagtacaacc gcaacctaac actgaaactt tcggtacaaa acctgatgaa
     8461 cagagattat tcggaggcgc tgaataagct caacatgatg ccaggtcttg gtgacgagac
     8521 ccacccagcc aattccgcgc gtggcagaac atggatattt ggcggggaca ttcgtttctg
     8581 atgacaattt ccgctaggtt cagacaatac gttttctctc ttatgtcaat tttattgcag
     8641 gaacgaaaaa tgaatatttt cactttatcc aaagcaccgc tatacctgtt aatttcacta
     8701 tttttaccca cgatggccat ggctatcgat ccacctgaac gcgaactttc gcgatttgcc
     8761 ctgaaaacga attaccttca gtcccctgat gaaggcgtct atgaactggc gtttgataat
     8821 gccagtaaaa aggtgtttgc agcagtcacc gatcgtgtaa atcgtgaagc caataaaggc
     8881 tatctgtatt cgtttaattc agattcgctg aaagtcgaaa ataaatacac gatgccatac
     8941 cgggcatttt cgctggcgat aaatcaggat aaacatcagc tctatatcgg acacacccag
     9001 tcagcgtccc tgcgtatcag tatgtttgac accccaaccg gcaaactggt aagaaccagc
     9061 gacaggttaa gttttaaagc ggcaaacgct gcagattcgc gttttgagca ttttcgccat
     9121 atggtttaca gccaggattc cgataccctg tttgtgagtt atagcaatat gctgaaaacg
     9181 gccgagggca tgaagcctct gcataagctg ttaatgctcg acgggacgac gcttgcctta
     9241 aaaggcgagg ttaaggatgc ttacaaaggt acagcgtatg gtctgacgat ggatgaaaaa
     9301 acacagaaaa tctacgttgg cggaagagat tacatcaacg aaattgatgc gaaaaatcag
     9361 acgctgctgc gtaccatccc gttgaaagat ccgagaccac aaatcacaag tgtgcagaat
     9421 ctggcggtgg actccgcttc tgaccgtgcc tttgtggtgg tattcgacca tgacgatcgt
     9481 tccggtacaa aagatggact ctatattttt gacttacgcg acggtaaaca gcttggctat
     9541 gtgcacacag gagccggagc taacgcggtg aaatacaatc cgaaatataa cgaactgtat
     9601 gtcaccaact tcactagcgg caccatcagc gtagtggatg ccaccaaata cagcatcacc
     9661 cgtgaattta acatgccggt ctacccaaac cagatggtgt tgtcggacga tatggatacc
     9721 ctttacattg gcatcaaaga aggctttaac cgcgattggg atcctgatgt gtttgtggaa
     9781 ggagctaaag aacgtattct gagcattgat ttgaaaaagt cgtgatcggg gggaaaaaac
     9841 atgggataaa aactccgtta ttccactaaa ctggccggac taggaaaagc aaacatctct
     9901 gtcctgccgg tacgcctgtt acgccgctcc atgaggctga ggaatctcca gaaaacgaga
     9961 caggcacagc attgtacgat caactgattt gtgccaataa ttaaatgaaa tcacagctat
    10021 gcctgttcat ggggttctag gccggaaccc cagatttttc cgattgtttg ttttctaact
    10081 gattattcag tagttttaga gggctgacgc tcagtggaac gaaaactcac gttaagaggt
    10141 tttgactatg ataaaagtca aagtaggggt gagtttggat atggaaatta ttgtactata
    10201 aggagagcat gaaaacccct ggggcagtca ctgaatgagg gaccagcagc ggtaaaataa
    10261 ccagcataat ttctgcatgg tcgattttta taccgtgaca ggctgttttt tatacttggg
    10321 gtcagtttgg atatcgaaaa ttattgtacg ttaaggttgt tttttgacct tgtcgatctt
    10381 aactgatctc aaacgacgaa acttacctga agtcggtatc cggctgtttt tccagatata
    10441 atcgcctgtc agattgatat gttcccaacc taatggtgat aaatgcgata atagttgagc
    10501 atttattttc atgcccttac gcttcagagc atcaaccgct ctttccatgt aaactgtgtt
    10561 ccataaggaa atagccgccg tcaataaagt caatccactg gcgcgataac tttgattttc
    10621 cggtttccga tctctaatct cccccaatcg atgcatgaat acagcacggg ccagagcatt
    10681 acgggcttct cctttgttca gcccggcctg gacacgtcgc cgcaatgccg gatcccgaaa
    10741 ccagtccagc ataaacagtg ttcgttctat acggccaatc tctctcagag ctttagccaa
    10801 cccattttgt ttgtaagcgt gcagcaagaa ccgtattgac gggaatgtgt tattcagtcg
    10861 gcagtgctac gcgccagggg agcagttcgc cgacccggtt tatcggccag tcggctatga
    10921 cgtcaaggac atagcggagg tagctttctg gctccactcc gttcagtttg cacgtcccga
    10981 tcaggctgta cagcagcgct ccccgctctc ctccatgatc cgaaccgaag aacaggtagt
    11041 ttttgcggcc cagactgacc atccgcaacg cattttcagc gatgttattg tccgcctcag
    11101 cccagccatc atctgcatag tacgtcagcg ccggccactg gttcagggcg tatgcgaacg
    11161 ctttcgccag ttctgagtgt cgcgacaggg ttttcatctt ttcacgcagc cagctttcca
    11221 gggatttcag cagcggtttc gttttcaact gacgttcggc aaggcgctgt tccgccgtca
    11281 ttccccttat ctctgcctcg atggcgtaca gttcgccgat ccgtttcagc gcttcctccg
    11341 tcagggctga cggggtgcga acgtgcacat cgtggatttt acggcgggcg tgagcccaac
    11401 aggcggcttc cgttatccgg ccatcccggt acagctcgtt gaacccggcg tatgcatccg
    11461 cctgcagtac accactgaac cccgcaagat gggtctgcgg atggatgcct tttctgtccg
    11521 ggctgtaagc gaaccacacc gccggcgcca gcgttgaccc ggcgttacgg tcgtcacgaa
    11581 catacgccca caaccgcccg gtcttcgttt tcttattgcc tggcaacagc accgggacag
    11641 gcgtgtcatc aacatggagc ttaccgtcag tcagcacata gccatgaagc gcctcttcca
    11701 tcggagacag cagccggcag catgcatcca cccagcccga cagcagtgaa cggctcagct
    11761 ccacaccttg ccggccgtat atttctgact ggcgatacag cggggtgtgc tctgcatact
    11821 tcgaggtcag cacgcgggcc agcagccccg gtccggcgat accccgctcg atgggccgcg
    11881 aaggtgcagg tgcctgcacg atggcatcgc actgagtaca ggcatgtttt tcccgtaccg
    11941 tccggataac ccggaaggcg ctacgcatca actccagctg ttcggcggta tcctcgccca
    12001 gatagctcag tgaaccgccg cagttcgggc agcacggcgc cgcaggcaac agtcgctttt
    12061 cgtcacgggg tagtgattca gggaacggct tacgggtgcg ggtctgacgc aacggacgct
    12121 gtactgccgg gtcatacacc ctaccagtca gcgtatcgct ctctttctga agccggttca
    12181 gatcggcttc catttgtgcg atacggcggg agactttttc ggaacgactg ccgaagttca
    12241 tccggcggag tttatccagc tgcgcctgca gatggtctat ttcgcgctcc cggttgctaa
    12301 gcttttcctg cagggcgtgg atcagcgctt cctgttcggc
//
LOCUS       DAKISA010000121        12217 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.121, whole genome shotgun sequence.
ACCESSION   DAKISA010000121 DAKISA010000000
VERSION     DAKISA010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12217
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.121"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            1..562
                     /locus_tag="OVW45_003586"
                     /pseudo
     CDS             1..562
                     /locus_tag="OVW45_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
     gene            885..2273
                     /gene="narU"
                     /locus_tag="OVW45_003587"
     CDS             885..2273
                     /gene="narU"
                     /locus_tag="OVW45_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HCV5527943.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIDDQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAITETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            2355..6095
                     /gene="narZ"
                     /locus_tag="OVW45_003588"
     CDS             2355..6095
                     /gene="narZ"
                     /locus_tag="OVW45_003588"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HCV5527944.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKIYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            6092..7636
                     /gene="narH"
                     /locus_tag="OVW45_003589"
     CDS             6092..7636
                     /gene="narH"
                     /locus_tag="OVW45_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCV5527945.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFKNTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7636..8331
                     /gene="narW"
                     /locus_tag="OVW45_003590"
     CDS             7636..8331
                     /gene="narW"
                     /locus_tag="OVW45_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCV5527946.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     ILSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            8328..9008
                     /gene="narI"
                     /locus_tag="OVW45_003591"
     CDS             8328..9008
                     /gene="narI"
                     /locus_tag="OVW45_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCV5527947.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRSLTNQRVRATSTTPDIIIMSILLIQCMLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            9087..9980
                     /locus_tag="OVW45_003592"
     CDS             9087..9980
                     /locus_tag="OVW45_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HCV5527948.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFELPLEGEVRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEI"
     gene            complement(10076..10921)
                     /gene="nhoA"
                     /locus_tag="OVW45_003593"
     CDS             complement(10076..10921)
                     /gene="nhoA"
                     /locus_tag="OVW45_003593"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HCV5527949.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQPMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            11094..11663
                     /locus_tag="OVW45_003594"
     CDS             11094..11663
                     /locus_tag="OVW45_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCV5527950.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSVREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11667..11894)
                     /gene="pptA"
                     /locus_tag="OVW45_003595"
     CDS             complement(11667..11894)
                     /gene="pptA"
                     /locus_tag="OVW45_003595"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HCV5527951.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIEPQMEALIKKPGYSM"
     gene            complement(11994..>12217)
                     /locus_tag="OVW45_003596"
     CDS             complement(11994..>12217)
                     /locus_tag="OVW45_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCV5527952.1"
                     /translation="ENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKV
                     FKAGLRRKMRKAAMGRNYLASVLTGSGLS"
BASE COUNT         2934 a         3126 c         3145 g         3012 t
ORIGIN      
        1 ttctgcaatg ttcagacatt aaacgctgct aacaaccagt tgaaaaatat aaatcttgat
       61 ttcctgactt gtcttccatc tattaaaagt ttaagactgg acaacaataa aataacccac
      121 attgatacta ataatacatc cgatattgga actttatttc ccataataaa acagagcaaa
      181 aacttaaatt ttttaaatgt ttctgggaag aacaattgcc ctactatgca gctcatgtta
      241 tttaatttgt tttccccagc acttaagctt aatactggcc tggcaattct ttcgcctggt
      301 gcatttgaag ttcactctga cggaatagat gtggataacg aattgtttca ctatcctatt
      361 aaaaatagca tataccccat ataatataca cacttacaag acagaggaag ttgtaaacca
      421 gaggaatata aaagttaaaa acatgacctt agatgaaata aacaatactt actgtaataa
      481 cgattattac aatcaggcaa taagagagga accgatagac cttctggaca gatcgttttc
      541 ctccagttca tggccttttt agtgaataaa tcatcactaa ttctataagt gtctggtctt
      601 gttgataata tagttaatat gtaacccttt agattcacga tctgaagggt tgaaacatga
      661 catacgttca aagattaacc ccgtttgaag gttagcttgt tagttacatt tagtaacaca
      721 tatctggcta cttaatcccc attctgcccc cgcggttacg ctttcttatc tattctttca
      781 atcagcactt cacccaattc atccttaatt ctaataactg gctattgcgc cttacctcct
      841 gttttgtctt ttttcattac caatgtgtgc atgtgaggaa caatatggca ctgcaaaatg
      901 agaaaaatag tcgttatctt ttacgcgact ggaaaccaga aaatccggcc ttctgggaaa
      961 ataaaggaaa acatattgct cgaagaaacc tctggatatc agtcagttgt ctacttcttg
     1021 ccttctgtgt ctggatgcta tttagcgcag ttaccgttaa tctcaataaa atcggtttta
     1081 attttactac cgatcaactc tttttattaa ccgcattacc ctccgtttct ggcgcattat
     1141 tgcgtgttcc ctactccttt atggtgccta tattcggtgg acgccgatgg acggttttta
     1201 gtactgcaat cctgattatt ccttgcgtct ggctcggaat tgccgtgcaa aatccgaata
     1261 ctccttttgg gatatttatc gttatcgctt tgctatgcgg ttttgcaggt gcaaactttg
     1321 cttcgagcat gggcaatatc agtttcttct ttccaaaagc caaacaaggg agcgctcttg
     1381 ggattaatgg cggattagga aacttaggtg taagtgtaat gcagctggtt gcaccgctgg
     1441 tcatttttgt acctgtattt gcctttctcg gcgtcaatgg cgtaccgcag gccgacggtt
     1501 cggtgatgtc gctggcgaat gccgcatgga tttgggtacc gctactggcg attgccacga
     1561 tcgccgcctg gtcagggatg aatgatatcg ccagttcacg cgcctcaatt gacgaccagc
     1621 tccctgtctt acaacgcctg catctctggc tgctgagcct gctttacctt gccaccttcg
     1681 gttcgtttat cggtttttct gcgggttttg ccatgctggc aaaaacccag ttcccggatg
     1741 tgaatattct gcgcctggcg ttctttggcc catttatcgg tgccatcgcg cggtcggttg
     1801 gtggtgctat ttccgataaa ttcggcggcg tgcgggtgac gttgatcaac tttattttta
     1861 tggcgatttt cagtgccctg ctgttcctta ccttaccggg cacaggctcc ggtaatttca
     1921 tcgcctttta cgccgtattt atggggctgt ttctgactgc gggtctggga agtggttcta
     1981 ctttccagat gatcgccgtc atctttcgcc agataaccat ttatcgggtg aagatgaaag
     2041 gcggtagtga tgagcaagct cagaaagaag ccatcaccga aacggcggcg gctctgggct
     2101 ttatctcagc cattggcgca gtgggcggct tttttattcc gcaggcgttt ggcatgtcgc
     2161 tcaatatgac cggctctccg gtcggcgcga tgaaagtgtt tttaatcttc tacatcgttt
     2221 gtgtgctgct gacctggctg gtttatggtc ggcggaagtt cagccaaaaa taagagatcg
     2281 atgcctgatg tacaaccgtg tcgcatccga caaggcatca gatcccaatt cattcctgga
     2341 gcaggagtca tgtcatgagt aaacttttgg atcgctttcg ctacttcaaa caaaagggcg
     2401 aaacctttgc cgatggtcac ggacaggtga tgcatagcaa ccgcgactgg gaggacagct
     2461 atcgccagcg ttggcagttc gacaaaatcg tgcgttccac ccacggtgtt aactgtacag
     2521 gctcctgtag ctggaaaatc tacgttaaaa atggtctggt gacctgggaa atccaacaga
     2581 ccgactaccc gcgcactcgc cctgacctgc ccaatcatga acctcgcggc tgcccgcgtg
     2641 gcgcaagtta ctcctggtat ctctacagcg ctaaccgcct gaaatacccg ctcattcgta
     2701 aacgactgat tgaactgtgg cgtgaagctc tcaagcaaca cagcgatccg gtactggcgt
     2761 gggcatcgat tatgaacgat ccgcaaaagt gcttaagcta caaacaagtg cgtgggcgcg
     2821 gcgggtttat ccgctccaac tggcaggaac ttaaccaact gattgccgcc gctaacgtct
     2881 ggaccatcaa aatctatggt ccggatcgcg ttgccggttt ctcgccgatc ccggcgatgt
     2941 cgatggtgtc atacgctgcc ggaacgcgtt atctgtcgct gttaggcggc acctgtttaa
     3001 gcttctacga ctggtattgc gacctgccgc ccgcctcgcc gatgacctgg ggcgaacaaa
     3061 ccgacgtgcc ggaatctgcc gactggtata actccagtta catcatcgcc tgggggtcta
     3121 acgtaccgca gacacgtacg ccggacgccc acttctttac cgaagtacgt tacaaaggca
     3181 ccaaaactat cgccattacc cctgactact ctgaagtggc caaattgtgc gaccagtggc
     3241 tggcaccgaa acaaggcact gatagcgccc tggcgatggc aatgggccat gtgattttaa
     3301 aagagtttca tctcgataat cccagcgact actttatcaa ctactgccgc cgctatagcg
     3361 acatgccgat gctggtaatg ctggaacctc gcgacgatgg tagctacgta cccgggcgca
     3421 tgatccgcgc gtctgacctg gtagatggac taggcgaaag caataatccg cagtggaaaa
     3481 ccgtggcggt taataccgca ggtgaattgg tagtgccgaa tggttcgatt ggtttccgct
     3541 ggggagaaaa aggcaaatgg aatctggaat ccattgccgc cggtaaggaa accgaattgt
     3601 cgttaaccct gctcggtcaa catgacgctg ttgcaggcgt ggccttcccc tactttggcg
     3661 gcattgaaaa tccgcatttt cgcagcgtaa aacacaatcc ggtgctggtg cgccaattgc
     3721 ccgttaaaaa cctgacgtta gtcgatggca acacctgtcc ggtggtcagc gtttatgatt
     3781 tggtgctggc gaattacggc ctcgatcgcg ggctggaaga tgaaaacagc gcgaaagatt
     3841 acgctgaaat caaaccgtac accccagcct ggggtgagca aattaccggc gtaccgcgcc
     3901 agtatattga aaccatcgcc cgcgaatttg ccgataccgc ccataaaacg catggacgct
     3961 caatgattat cctcggcgct ggcgtcaacc actggtatca catggatatg aactaccgtg
     4021 ggatgatcaa tatgctgatc ttctgcggtt gtgtcgggca aagcggcggc ggctgggcgc
     4081 actatgtcgg tcaggaaaaa ctgcgcccac aaaccggctg gttgccgctg gcctttgcgc
     4141 tcgactggaa ccgcccaccg cgccagatga acagtacctc gtttttctac aatcattcca
     4201 gccaatggcg ctatgaaaaa gtctctgctc aggagttact ttcaccgctt gccgatgcca
     4261 gtaagtacag cggtcatctg attgatttca acgttcgcgc cgaacgcatg ggctggctac
     4321 cttctgcgcc gcagctgggg cgtaacccgc tcgggattaa agctgaagcc gacaaggccg
     4381 gattatcccc cacagaattt accgcccagg cgctgaaatc gggcgattta cgtatggcct
     4441 gcgaacaacc agatagcggc agcaatcatc cgcgtaattt gtttgtctgg cgctctaacc
     4501 tgcttggctc ctccggcaaa ggccacgagt atatgcagaa gtatctgctg gggaccgaaa
     4561 gcgggattca gggcgaggaa ctcggtgcca gcgacgggat caaaccggaa gaagtcgagt
     4621 ggcaaactgc ggcgattgaa ggcaagctcg acctgctggt gacgctcgac ttccgcatgt
     4681 ccagtacctg cctgttctcc gatatcgttc tgcccaccgc cacctggtac gaaaaagacg
     4741 atatgaacac ctcggatatg catccgttta ttcatccgct ttctgcggcg gtcgatccgg
     4801 cgtgggaatc acgcagcgac tgggaaatct acaaaggtat cgccaaagca ttttcgcaag
     4861 tgtgcgtggg tcatcttggc aaagaaaccg acgtggtatt acaaccccta ctgcatgact
     4921 ctccggcaga gctctcacag ccgtgtgaag tgctcgactg gcgcaaaggc gaatgcgatc
     4981 tgattccagg caaaaccgcg ccgaatattg tggcggtgga gcgcgactac cctgctacgt
     5041 atgaacgctt tacctcgctc gggccattga tggacaaact tggcaatggt ggtaaaggga
     5101 tttcgtggaa tacccaggat gaaatcgatt tcctcggcaa actcaattac accaagcgtg
     5161 atggcccggc acaggggcgt ccgctgattg acaccgccat tgacgcttca gaagtgattc
     5221 tggcactggc accggaaacc aacggtcatg ttgcagtcaa agcgtggcag gcgctgggcg
     5281 agatcaccgg gcgcgaacat acccatctgg cgctgcacaa agaggacgag aagattcgct
     5341 ttcgcgatat tcaggcgcag ccgcgtaaaa ttatctccag ccccacatgg tccggtctgg
     5401 aaagcgatca cgtctcctat aatgcgggat acaccaacgt tcatgagtta attccgtggc
     5461 gcacgctgtc gggacgccag cagctctatc aggatcatcc gtggatgcgt gcttttggtg
     5521 aaagcctggt ggcttatcgc ccgcctatcg acacccgtag cgtcagtgag atgcgccaga
     5581 tcccgccaaa cggcttcccg gaaaaagcac ttaacttcct gacgccgcac cagaaatggg
     5641 gcattcactc aacctacagt gaaaacctgc taatgctgac gctctctcgc ggtggaccga
     5701 ttgtctggat cagcgaaacc gatgcccgag aactgaccat tgtcgataac gactgggtgg
     5761 aagtctttaa cgccaacggc gcgctgacgg ctcgtgcggt ggtcagccaa cgtgtaccgc
     5821 cgggtatgac catgatgtat cacgcccagg aacgcattat gaatattcct ggttcggaag
     5881 taactggcat gcgcggcggc attcataact cggtcacccg tgtttgcccg aaaccgacgc
     5941 atatgattgg cggttacgcg cagctggcct ggggctttaa ctactacggc accgtcggct
     6001 cgaaccgcga cgaattcatc atgatccgca agatgaagaa cgttaactgg ctggatgatg
     6061 aaggtcgcga tcaggtacag gaggcgaaaa aatgaaaata cgttcgcaag tcggcatggt
     6121 gcttaacctc gacaaatgta tcggttgcca tacctgttcg gtgacctgta aaaacgtctg
     6181 gaccggacgc gaaggcatgg agtacgcatg gtttaacaac gtcgaaacca aaccgggcat
     6241 tggttatccg aaaaactggg aagatcagga agagtggcaa ggcggctggg tccgcgatgt
     6301 gaatggcaag atacgcccgc gtctgggcag caagatgggc gtaataacca aaatcttcgc
     6361 caacccggtg gtgccgcaga ttgatgatta ctacgaacct ttcaccttcg actacgaaca
     6421 tttgcatagc gcaccggaag gcaaacatat ccctactgct cgcccgcgtt cgctgattga
     6481 cggtaagcgg atggacaaag tgatctgggg gccaaactgg gaagaactgc tgggcggcga
     6541 gtttgaaaaa cgtgcccgcg accgcaactt cgaggccatg caaaaggaga tgtacgggca
     6601 gtttaaaaac accttcatga tgtacctgcc gcgcctgtgc gaacactgcc tcaatcccag
     6661 ttgcgtggcg acctgcccaa gcggcgctat ctacaaacgc gaagaagacg gcattgtgct
     6721 gattgatcag gataaatgcc gtggctggcg tttgtgcatt agcggttgtc cgtacaaaaa
     6781 aatctacttc aactggaaaa gcggcaagtc agaaaaatgt atcttctgtt atccgcgaat
     6841 tgagtcaggc caacccaccg tgtgctcaga aacctgcgtg ggtcgcatcc ggtatctggg
     6901 cgtgctgctt tacgacgccg accgcattga ggaagcggcg agcaccgagc gcgaagttga
     6961 cctctatgaa cgtcagtgcg aagtgttcct cgatccacac gatccctcag tgatcgagga
     7021 agccctgaaa caaggtattc cacaaaacgt gattgaagct gcccagcgtt cgcctgtcta
     7081 caaaatggcg atggactgga aactggcgct accgctgcac cctgaatatc gcaccctgcc
     7141 aatggtctgg tacgttcctc cgctgtcgcc gattcagtcc tacgcagatg cgggcggttt
     7201 gccgaaaagc gaaggcgtgc tgcccgccat cgaaagcctg cgtattccgg tgcaatatct
     7261 cgccaatatg ttgagtgccg gcgataccgg tccggtactg cgggcgctga aacggatgat
     7321 ggcgatgcgc cactatatgc gttcacaaac cgtggaaggc gttactgata ctcgtgccat
     7381 cgacgaagta ggcctgagcg tcgcccaggt cgaagaaatg tatcgctatc tcgccattgc
     7441 caactatgaa gatcgttttg ttatcccgac cagccatcgg gaaatggcgg gcgatgcctt
     7501 cgcagaacgc aacggctgcg gttttacctt tggcgacggt tgccacggtt cggacagtaa
     7561 attcaacctg ttcaacagta gccgtatcga tgccatcaac atcaccgaag tgcgcgacaa
     7621 agcggagggc gaataatgca gatcctcaaa gtgatcggcc tgttgatgga gtatccggat
     7681 gagctgttgt gggaatgcaa ggaggacgcg ctggcgttga tccgccgcga cgcgccgatg
     7741 cttacggatt tcacccacaa cctgcttaac gcgccgctgc tggataaaca ggccgaatgg
     7801 tgcgaagtgt ttgaccgcgg gcgcaccacg tcgctgctgc tgttcgaaca tgttcatgcc
     7861 gagtcccgcg atcgcggcca ggcaatggtg gacctgctgg cggagtatga aaaggtcggc
     7921 ctgcagctgg attgtcggga actgcccgat tatctaccgc tgtatctgga gtatttaagc
     7981 gtactgcctg acgatcaggc gaaagaagga ttgctcaatg ttgcgccgat cctcgccctg
     8041 cttggcggtc gcttaaaaca acgcgaggca ccgtggtacg cgttgtttga tgctctgctg
     8101 caactggcgg gaagtatact ttcaagtgac agtgtcacaa aacaagtaaa cagcgaagag
     8161 cgcgatgaca cccgccaggc gcttgatgca gtgtgggaag aggaacaggt gaagtttatt
     8221 gaagataacg ccacggcgtg tgacagctcg ccgcttaatc aatatcagcg acgctttagc
     8281 caggatgtcg cgccgcagta tgtcgacatc agtgcgggag gtgggaaatg attcagtatc
     8341 tgaacgtctt tttttacgat atctacccgt acatttgtgc gacggtgttt ttcctcggca
     8401 gctggctgcg ctacgactac gggcagtaca cctggcgcgc ctcctcaagt caaatgctcg
     8461 ataaacgcgg gatggtgata tggtcgaatc tgttccatat cggcattttg gggattttct
     8521 tcgggcactt gttcggcatg ttaacgccgc actggatgta cgcgtggttt ttgccagtgg
     8581 cagcgaaaca actcatggcg atggtgctcg gcggtatttg cggtgtgttg acgttgattg
     8641 gcggcgcagg actgctgtgg cgcagtctga caaatcagcg cgtgcgtgcc acttccacta
     8701 cgccggatat catcatcatg agcattttgc tgatccagtg tatgttgggg ctaagtacca
     8761 taccgttttc agcacagtat cctgatggta gcgaaatgat gaagctggtg ggttgggcgc
     8821 aaagcattgt cactttccgt ggtggatcgt cagagatgct caacggcgta gcgttcgtct
     8881 tccgcctgca tctggtgttg ggaatgacca tcttcctgct cttcccgttc acccgactgg
     8941 tgcatgtgtg gagcgcgccg tttgagtact ttactcgtcg atatcaaatt gtgcgttcgc
     9001 ggcgataatt tcaccctggc cggatagccc ttcgctctcc ggccacttgc aggctatgat
     9061 agttctccag tcacaggaga acgttaatga aaccgcaggt gtaccacgtc gatgctttta
     9121 cctctcaacc atttcgcggc aattctgccg gtgttgtttt ccccgccgat aatcttagcg
     9181 aagcacaaat gcagcttatc gcccgcgagt taggccattc ggaaactgct tttctgctgc
     9241 acagcgacga cagcgatgtg cgcatccgct actttacgcc aacggttgaa gtgccgattt
     9301 gcggtcacgc gacagtagct gcgcactatg tacgtgcgaa ggtgttaggt ttaggaaatt
     9361 gcacgatctg gcaaacatcg ctggcaggaa aacatcgcgt gactatcgaa aagcacaacg
     9421 atgattatcg tatttcgctg gaacaaggta cgccgggctt tgaactgccg ctggagggtg
     9481 aagtacgcgc ggcgattatc aacgcattgc atcttactga agacgatatt ctgccgggtt
     9541 tgccgatcca ggtcgcgacg accgggcatt ctaaagtgat gatcccattg aagccggaag
     9601 tggatatcga cgctctttcg cctgacctta atgcgctgac cgctatcagt aaacagattg
     9661 gctgcaatgg tttcttcccg ttccagatcc gtccaggcaa aaacgaaacc gatggtcgta
     9721 tgttctcgcc tgcgattggt attgtggaag atccggtgac tggcaatgcc aatggcccga
     9781 tgggtgcatg gttggtacat cacaacgtat tgccccacga tggcaacgtg ttgtgtgtta
     9841 aaggccatca ggggcgcgca ttggggcgtg acggcatgat tgaagtgacg gtgacaattc
     9901 gtgataacca accggaaaaa gtcaccattt caggcacagc ggtgattttg tttcatgccg
     9961 aatgggcaat tgaaatctga aatcgttgcc tgatgcgctt cgcctgtcag gtctacaacc
    10021 gggctaatgt atgccggata aggcgtttac gccgcatccg gcaacctgac ataaattatt
    10081 ttcccgcctc cggatgcgta tcaaacgccg ccatcaccag cgctaactca tccacagtaa
    10141 agccatgttt cgcatcatcc acgcccagac caaactgttc ctgcatcacg gcatataacg
    10201 acgccacatc cggtagattt cgctgctcca ccgcgtgccc attttcataa tgggtaaaat
    10261 gaaaattggt cagtgtcagc ttgccgccgt ccggcaaatg gcggcacatc agcaaatgat
    10321 ggcgaaaatg cgactgcggc cagtgcgccg accagaaatt gcccatcaca taatcgcttt
    10381 gttgctgctc gcagagatcg aagcggtaca tcggctgcca atgctgatga tgattaaact
    10441 gcaacaccca atcatcaccc tcctgcaaca accgatactc tccgtgtggc gtggtctgca
    10501 cgagatcgga aactaaacga atcggcgcgg ttagcgtctg cccaccgaaa ccgacatcag
    10561 caatccattt ttccccttcc agttccacca acagcaaacg atgggtgcgc ggcggtaatg
    10621 ctggcggatt tgataacact acgcgcccta acaagctgcg aacgttaaac cccagctcgc
    10681 gtaacacccg ctcaaacacg ccattctgct caaaacagta accgccacga cgggctatca
    10741 ccagtttctc ttccagcgat tgatcatcaa gctgtatttc cctcggcagc aaaacgtcaa
    10801 ggttttcaaa cggaatggtg caattgtgtt tcaggtgcaa tgcacgaagc gtatcaatat
    10861 tgaccgcagc agctcccgac cagttaatac gggcaaaata gtgattcaga atgggcgtca
    10921 tcaggcgttt cccttagtgg ttttctcaac ttataacctg gaatagacgc cgcaccggct
    10981 attttgtgcg atcgcggaca tgcatcacgt ttgcaaatcg ccaatcccgt taatttcgtt
    11041 acttattgcg ttacggacta cgcttaaaaa aaccttcttc gcgaggcgat accatgagcc
    11101 gattcatccc catagaatta caccatgcca gccgtctgtt aaatcatggc ccaactgtct
    11161 tgatcaccag tttcgatgag caatcccagc ggcgtaacat tatggctgca gcctggtcaa
    11221 tgccggtgga gtttgaacct ccacgcgtgg caattgtggt agataaatcg acatggacca
    11281 gggagttgat tgaacgtaac ggtaagtttg gcatcgttat cccgggcgtt gcagcaacta
    11341 actggacgtg ggcggtggga agtgtgtcgg tgcgtgaaga agataaattt aattgctatg
    11401 gcattccggt ggtgaaaggt ccggtgcttg gtttgccact tgttgaagag aaatgcctgg
    11461 cgtggatgga gtgccgcctg ctacctgcga cttctgcgca acaaaaatac gacacgctgt
    11521 ttggcgaggt ggtatcagca gcggcagacg cacgggtatt tgtcgaaggc cgctggcagt
    11581 ttgatgatga taagctcaat acgttgcatc atttaggtgc tggcacgttt gttactagcg
    11641 gcaagcgtgt tacggcaggt taagcattac atgctgtaac cgggtttctt tatcaaagcc
    11701 tccatttggg gctcgatttc ggcatcccag atagcttgcc aagattctgg ttgaatctgc
    11761 tgtagagcaa tgcttatcga gctatcttta ctgttcagat ggcgaataat aacgtcggta
    11821 atatctgcag caagtgctgc tttttgttgt tcgtccagtt cacgcggaaa gcatttaatg
    11881 tcgatgtgcg gcatgattac gctccttttg aaagatgtca gtaaaattaa cacacattgt
    11941 gaatgtcatg tgtccggtaa agcgtttata cgacaataat gcccagggca agattacgaa
    12001 agcccgctcc ccgtaaggac tgacgccagg tagtttctgc ccatggctgc ttttcgcatc
    12061 ttacgtctta accctgcctt gaatacctta tcattcgtca aaatattaat agcaatgtgc
    12121 cgtatccctg aaaataattc tgctgcattt cctcttctta ttttgcagtc gtcttcattc
    12181 attaccacgt ccagacgcca atgcagctta ttctcca
//
LOCUS       DAKISA010000122        12080 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.122, whole genome shotgun sequence.
ACCESSION   DAKISA010000122 DAKISA010000000
VERSION     DAKISA010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12080
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.122"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..953)
                     /gene="ydcO"
                     /locus_tag="OVW45_003597"
     CDS             complement(<1..953)
                     /gene="ydcO"
                     /locus_tag="OVW45_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HCV5527953.1"
                     /translation="MLRCLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTNVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLLAGLFG"
     gene            1006..1542
                     /gene="sutR"
                     /locus_tag="OVW45_003598"
     CDS             1006..1542
                     /gene="sutR"
                     /locus_tag="OVW45_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HCV5527954.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSAMPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1615..3576
                     /gene="rlhA"
                     /locus_tag="OVW45_003599"
     CDS             1615..3576
                     /gene="rlhA"
                     /locus_tag="OVW45_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HCV5527955.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVFPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(3668..3898)
                     /locus_tag="OVW45_003600"
     CDS             complement(3668..3898)
                     /locus_tag="OVW45_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HCV5527956.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            4120..4274
                     /locus_tag="OVW45_003601"
                     /pseudo
     CDS             4120..4274
                     /locus_tag="OVW45_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
     gene            4320..4736
                     /gene="hicB"
                     /locus_tag="OVW45_003602"
     CDS             4320..4736
                     /gene="hicB"
                     /locus_tag="OVW45_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HCV5527957.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            4815..6221
                     /locus_tag="OVW45_003603"
     CDS             4815..6221
                     /locus_tag="OVW45_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCV5527958.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRE
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQDYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVLGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHSDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            6466..7611
                     /locus_tag="OVW45_003604"
     CDS             6466..7611
                     /locus_tag="OVW45_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCV5527959.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            7629..8642
                     /locus_tag="OVW45_003605"
     CDS             7629..8642
                     /locus_tag="OVW45_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCV5527960.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            8643..9543
                     /locus_tag="OVW45_003606"
                     /pseudo
     CDS             8643..9543
                     /locus_tag="OVW45_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            9533..10327
                     /locus_tag="OVW45_003607"
     CDS             9533..10327
                     /locus_tag="OVW45_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCV5527961.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGSVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     LLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            10349..11773
                     /gene="patD"
                     /locus_tag="OVW45_003608"
     CDS             10349..11773
                     /gene="patD"
                     /locus_tag="OVW45_003608"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HCV5527962.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            11983..>12080
                     /locus_tag="OVW45_003609"
     CDS             11983..>12080
                     /locus_tag="OVW45_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HCV5527963.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNK"
BASE COUNT         2945 a         2915 c         3224 g         2996 t
ORIGIN      
        1 ccaaacagac ctgcgagcaa atagaaaatg cctgcaacgg cagctgccag ccaacgttga
       61 tctttatccg gatgcgcttc cgggctttgg caaatagccg cggtgattgc cgcaataccg
      121 acggaataaa cgccgaaagg ggaaaaaacc agtgccagca atccagtaaa tacaattaat
      181 ggcgaaacag gagccgaata tccagctgct ttcattgctg cgatacccgg tgcgttttgc
      241 gatgccatcg tcaccagaaa aagggggagt gcaacgctca ggctgtgagc aaacgaaaaa
      301 tcaggggtaa tataagtggg gagaacgggt ttaaagacaa cattagttgt gacaacgtca
      361 ccttgcgcga taacgatcac gatcccaata atcatcgcgg caattaccgc atagcgcggc
      421 gcaacggcct tggttgccag ccataccagc aacatacttc cacacaacgt aaattgaccg
      481 tccagactgg caaacgcctg taaaccaaag cgtaataaaa tcccggcaag cattgccgcc
      541 gcaagcgagt gcggaataat gcgcatcaga cgagcaaaga gtcccgttat gccgcagagg
      601 actattaacg cgttggtgac aataaaaacg ccgatggctt cgttaagtgt tagtccctgc
      661 aatccggtga ccaacaaagc cgcgccaggc gttgaccatg cggtgagaac aggtacgcga
      721 taccataatg tcagagtcag cgtactgacg cccattgcca gccccagcgc cgtcatccag
      781 ccagagattt gtgcagtggt ggctccggcg acaatcgctg cttgccagat tattgccgct
      841 gaactggcgt agccaattaa taccgccaga aaccccgcca gtagcgtggg tggaggaata
      901 gaaaacagac gcatagtcac ctcgtgcgtt atagcgtaca ggcaccgtag catttgtccg
      961 ttataacgca caagtgataa acttccgttt tgccggagga gtcgcatgga aaatctcgct
     1021 cgctttttat ccactacact taaacaacta cgccagcagc gcggctggag tctttcgcga
     1081 ctggcagaag cgacgggcgt ttctaaagca atgttagggc agattgagcg taatgagtcc
     1141 agtccgacgg tcgcgacatt atggaaaatt gccaccgggt tgaacgtacc attttcaaca
     1201 tttatttctc cgccgcagtc cgctatgcct tcagtttatg atccacaaca gcaggcgatg
     1261 gtgatcactt cgctgtttcc ttatgatcca caactctgct ttgaacactt ctcgattcag
     1321 atggcatctg gtgcaataag cgaatcgacg ccccatgaga agggggtaat cgaacatgtc
     1381 gtcgtcattg atggacaact tgatctgtgc gttgatgggg aatggcagac ccttaattgt
     1441 ggggaaggcg ttcgatttgc tgcagacgtc acgcatatct atcgtaatgg tggggagcaa
     1501 accgtacatt ttcattccct catccattac ccgcgcagtt aagcgggaaa actatttcgc
     1561 aacgtcgcgc ttgtggctaa aatagccgcc atttttcagc tactggataa gaatgtgacc
     1621 gtatcttctc atcgacttga actgttaagc ccggcacgcg atgccgccat tgcccgcgaa
     1681 gctattttgc acggtgccga tgctgtttat atcggcggcc ctggttttgg tgcccgtcat
     1741 aatgccagta atagcctgaa agatattgcc gagctggtgc cgtttgccca tcgttatggt
     1801 gcaaaaattt tcgtcacgct taacaccatt ttgcatgatg atgagctgga acccgcgcaa
     1861 cggctgatta ctgacctcta ccagaccggt gtcgatgcgc tgattgttca ggatatgggg
     1921 attctggaac ttgatattcc gccgattgaa ctgcacgcca gtacgcagtg cgacattcgt
     1981 acagttgaaa aagcgaagtt cctctctgat gttggcttca cgcagattgt gctggcgcga
     2041 gagctgaatc ttgatcagat ccgcgcgatt caccaggcta cggacgcgac cattgaattc
     2101 tttattcatg gggcactgtg cgtggcctat tcgggtcagt gctacatttc tcatgcgcaa
     2161 acagggcgta gcgccaaccg tggcgattgc tcgcaggcgt gccgtttgcc atacacattg
     2221 aaagacgatc aggggcgggt ggtttcctat gaaaaacatc tgctgtcgat gaaagataac
     2281 gatcagactg ccaacctcgg cgcgctgatt gatgctggcg tacgctcctt caagattgaa
     2341 gggcgttaca aagatatgag ctacgtgaag aatatcaccg cccattatcg ccagatgctg
     2401 gatgccatta ttgaagaacg tggcgatctg acgcgtgctt catcaggtcg tactgaacat
     2461 ttctttgttc catcgacgga aaagactttc caccgtggta gcacagatta ttttgtgaat
     2521 gcccgtaaag gcgatattgg cgcgttcgat tcgccgaaat ttatcggcct gccggtgggt
     2581 gaagtattga aagtggcgaa agatcatctc gatgttgccg tcaccgagcc actggcaaat
     2641 ggtgatggcc tgaacgtgat gattaaacgt gaagtcatcg gttttcgtgc caatacggtc
     2701 gagaaaaccg gagaaaatca gtaccgcgtc tggcccaatg aaatgccagc agatttgcac
     2761 aaaattcgtc cacatcaccc actaaaccgt aatcttgatc ataactggca gcaggcactg
     2821 acaaaaacct ccagcgaacg tcgggtggcg gtagacattg aactgggcgg ctggcaggaa
     2881 caactgattc tgaccctcac cagtgaagag ggtgtcagca tcacgcatac gctggacggg
     2941 cagttcgacg aagccaataa cgccgaaaaa gcaatgaaca atctgaagga tggtctggca
     3001 aaactggggc aaaccctcta ttacgcccgc gatgtgcaaa ttaatttgcc gggggcgctg
     3061 tttgtaccaa acagtctgtt aaaccagttc cgccgtgaag ctgctgacat gctggatgct
     3121 gcgcgtcttg ccagttacca gcgcggcagc cgtaaaccgg ttgctgatcc tgcgccggtt
     3181 tttccgcaaa cgcatctgag tttcctcgcg aacgtataca accagaaagc gcgtgaattt
     3241 tatcatcgct atggtgtgca gctgattgac gcggcgtatg aggcacatga agagaagggc
     3301 gaagtcccgg tgatgatcac caagcattgt ctgcgctttg cctttaatct gtgcccgaaa
     3361 caggcgaaag gcaatatcaa aagctggaag gcgacgccaa tgcaactggt taacggcgat
     3421 gaagtattaa cgctaaagtt tgattgccgc ccatgcgaga tgcacgtcat tggcaaaatc
     3481 aaaaatcaca tactgaaaat gccgttaccg ggaagcgtag tggcatccgt aagtccggat
     3541 gagctgctga aaacattgcc taagcgaaaa gggtaaaacg ccagttttct ggttactcac
     3601 aactcattga atctgcatga tattgcctgc cgggtaaggc gtcacgccgc atccggcatc
     3661 aaacgactca acgttgtccc gcttctggtt tgcgcgattt ttgccagtaa aaatgctcgc
     3721 gcaaaccttc cgccgactct tccgccacag cacgcaattc atcgctgtcc gcttcatgac
     3781 gcagctgatg atccacattc tttacccaca caaattcatg tcctttgtgc cctgccatga
     3841 gttgtcctga gaacagcgca cacgttaata agacaaccga taacgccttc gtaaacatcc
     3901 tgccaccttt ttcttacctt ttgccgctat gatgccgatc gtttcttgag gttattattc
     3961 agttttgcaa attagcgcaa agaaattctg gaatcttcct tcctgatttt gcattgcatt
     4021 ctgccgttgc ggcgatttag tgctattttc gagcaaatta cacatagggg taaacactaa
     4081 tgtttacttt attgatatac tcagcggcag ggaggcgatg tgaaacaaag cgagttcaga
     4141 cgttggctcg aatctcaggg cgtcgatgta gcgaatggcc gcaaccattt gaaactcagg
     4201 tttcatggga ggcgcagtgt catgccgcgt cactcctgcg taaagcaatc ctgaaacaac
     4261 tcggtttgag ttaatcgcca attaaaaagg ttaatgacat gcgagagaca gtcgaaatta
     4321 tgcgttatcc cgtcactctt acacccgcgc cggaaggcgg ttatatggtt tcttttgtgg
     4381 atatccctga agcgttgacg cagggcgaaa ctgtcgctga agcgatggaa gcggcaaaag
     4441 atgctttact gaccgcattt gatttttatt ttgaagataa cgagcttatc ccgttacctt
     4501 cgccattaaa tagtcatgat cactttattg aagtaccttt gagcgtcgcc tctaaagtat
     4561 tgctgttaaa tgctttttta cagtcagaaa tcactcagca agagttagcc aggcgaattg
     4621 gcaaacctaa acaggagatt actcgcctat ttaacttgca ccatgcgaca aaaatcgacg
     4681 ccgtccagct tgcggcaaag gcgcttggca aagagttatc gctggtgatg gtttaattac
     4741 agttaacgaa aagttgtcat ttttaacaac tgatatagac tgccgaatca tctgcacata
     4801 attacgattc gataatgaaa aaataccagc agcttgcaga acaattacgc gagcagattg
     4861 cgtcgggtat ctggcaaccc ggcgatcgtt tgccttcgtt gcgtgaccag gtggcgcttt
     4921 caggcatgag ctttatgact gtcagccatg cctatcagtt gctcgaaagt cagggatata
     4981 ttatcgcacg accgcagtcg ggttattacg ttgcgccaca ggcaataaaa atgccgaaag
     5041 cgccagtcat tccagtcact cgagatgaag cagtcgatat caacacttat atttttgata
     5101 tgttgcaggc cagtcgcgaa ccgtcggtcg ttccgtttgc ctcggccttt cccgacccgc
     5161 gacttttccc cctccaacaa ctaaaccgct cgctggcgca ggtaagcaaa accgccacag
     5221 cgatgagcgt gattgaaaac ttaccgccag gaaacgcaga actgcgtcag gctattgctc
     5281 gtcgctatgc cttacagggc atcaccattt ctcctgatga aattgtcatt actgccgggg
     5341 cgttagaggc attaaacctc agtttgcaag cggtaactga accgggcgat tgggtgatag
     5401 tagagaatcc ttgtttctac ggtgcgttgc aggcgctgga gcggctacgg ctgaaggcgt
     5461 tatcggtggc gacggatgtt aaagaaggga tcgatcttca ggcgctggaa ctggcgttgc
     5521 aggattatcc ggtgaaagcg tgctggctga tgactaatag ccagaaccca ctcggattta
     5581 ccttaacgcc gcaaaaaaaa gcacaactgg tggcgttact caatcagtac aacgtaacgc
     5641 tgattgaaga tgacgtttac agcgaacttt attttggacg ggaaaaaccg ctgcctgcga
     5701 aagcgtggga tcgccacgat ggcgttttgc attgctcttc gttttcgaaa tgtctggtgc
     5761 ttggttttcg tattggttgg gtcgccgccg gaaaacatgc acgtaaaatt caacgcttgc
     5821 agttgatgag tacgctttcc accagctcac cgatgcaact tgcgctggtg gattaccttt
     5881 ccacgcgccg atacgacgcc catcttcgtc gcctgcgtcg ccagcttgcg gaacgtaaac
     5941 aacgtgcctg gcaggcactg ctgcgttatc tgcctgcgga agttaaaatt catcatagtg
     6001 acagcggtta ttttctctgg ctggagctcc ccgagccgtt agatgccggc gaattaagcc
     6061 tggcggcact gacgcatcat atcagtattg cgccgggtaa aatgttttct accggtgaaa
     6121 actggtcacg tttttttcgt tttaataccg cgtggcagtg gggagaacgt gaagaacagg
     6181 cggtaaaaca attaggcaaa cttattcaag aacggctgta atagcgttta atttaattcc
     6241 tcttagattg ggtaatatga atttcgaata gcagtcatat ttcctaaccc cttgtctata
     6301 ctccagaaga taaccttaca gacggcataa tgcgcggtag ctcacaacct gattaaattt
     6361 tctcagggac gaaggtgtgc ctgcaagccg ccgtctatgg ttaaacaagg agatattttt
     6421 acggcacggc ggctgaacaa ttaattacga caggagtaag accttatgag caagacattt
     6481 gcccgcagca gcctgtgtgc gctcaccatg acaataatga ccgctcacgc cgccgaacca
     6541 cctaccaatt tagataaacc ggaagggcga ctggatatta tcgcctggcc gggatacatc
     6601 gaacgcggac aaactgataa acaatacgac tgggtaacgc aatttgaaaa agagacaggc
     6661 tgcgcggtga atgtgaaaac cgccgcgact tccgatgaaa tggtcagtct gatgaccaaa
     6721 gggggttacg atctggttac ggcatccggc gatgcctcgc tgcgtttgat tatgggcaaa
     6781 cgcgtgcagc cgattaatac cgccttgatt cccaactgga aaacgctcga tccgcgcgtg
     6841 gttaaaggcg actggtttaa tgttggcggc aaagtttacg gcacacctta ccaatggggg
     6901 ccgaacctgc tgatgtacaa cactaaaacc ttcccgacgc cgccggatag ctggcaagtg
     6961 gtttttgttg agcaaaatct gccggacggc aagagcaata aaggccgcgt tcaggcttat
     7021 gatggcccta tctacattgc ggacgctgcg ttgttcgtta aagccactca gccgcagttg
     7081 ggcatcagcg atccgtatca actaaccgaa gaacagtacc aggcggtgct gaaagtgctg
     7141 cgcgctcaac acagtttgat ccatcgctac tggcatgaca ctaccgtgca aatgagcgat
     7201 ttcaaaaacg agggtgtggt tgcttccagt gcctggccct atcaggccaa cgccctgaaa
     7261 gccgaaggcc agcctgttgc taccgttttc ccgaaggagg gtgttaccgg ttgggctgat
     7321 accaccatgc tgcatagcga agcgaaacat ccggtttgcg cctacaaatg gatgaactgg
     7381 tcattaacgc caaaagtgca gggcgatgtg gcggcctggt ttggctcgtt accggtagtg
     7441 ccggaagggt gtaaagccag tccgttatta ggcgaaaaag gttgtgaaac caacggtttt
     7501 aactatttcg acaaaatcgc cttctggaaa acgcctatag cagaaggggg caagtttgtt
     7561 ccctacagtc gctggacgca ggattacatt gccattatgg gcggtcgcta acttcgctgg
     7621 ggtgctttat gacgtacgca gtggagtttg acaacgtctc gcggttgtac ggtgacgtgc
     7681 gcgcagtaga tggcgtcagt attgcgataa aagatggtga gttcttctct atgctggggc
     7741 cgtccggctc cggcaaaacc acctgcctgc gcctgattgc tggcttcgaa cagctttccg
     7801 gcggggctat ctctatcttt ggtaaacccg ccagcaatct gccaccgtgg gagcgggacg
     7861 tgaatactgt ctttcaggac tacgcgctat ttccgcatat gtcgattctt gacaatgtcg
     7921 cctatgggct gatggtcaaa ggcgtgaata aaaagcagcg gcacgcaatg gcgcaagagg
     7981 cgctggagaa agtggcgttg gggtttgtac agcaacgtaa accgtcacaa ctttctggtg
     8041 gtcagcgcca gcgggttgct atcgccagag cattggtgaa tgaaccgcgc gtattgctgt
     8101 tggatgaacc gctcggcgca ctggatctca aattgcgtga gcagatgcag ctggaactga
     8161 aaaaactgca acagtctctc ggtatcactt ttatcttcgt tacccacgat cagggcgaag
     8221 cgttatcgat gtccgatcgt gtggcggttt tcaataatgg acgcattgag caggtcgatt
     8281 ccccgcgcga tctctatatg cgcccgcgca cgccgtttgt tgccgggttc gttggtacat
     8341 cgaatgtttt tgatggactg atggcagaga aactttgtgg catgacggga agcttcgccc
     8401 tgcgaccgga acatatccgc ctcaacaccc ctggtgaact gcaggccaat ggcacgatcc
     8461 aggcggtgca atatcagggc gcggcaactc gttttgaact gaaattgaac ggcggtgaaa
     8521 aactgcttgt gagtcaggcc aatatgacag gcgaagaact gcctgccacg ctcacgcccg
     8581 gacaacaggt gatggtttcc tggtcgcgtg atgtgatggt gccgctggtt gaggagaggt
     8641 gaatggcgat gaatgtattg caatcacctt cacgtccagg tctggggctg tttttactgc
     8701 tgcttggccc gctaatgtgg tttggcattg tctatttcgg ctcgctgctg acactgttat
     8761 ggcagggatt ttatactttt gacgatttca ccatgtcggt aacgccggaa ctgacgctgg
     8821 cgaatatccg tgcgctgttt aatccggcga attacgacat cattctccgc acgctgacta
     8881 tggctgtggc agtcactatc gccagcgcca ttctggcttt tccaatggcg tggtatatgg
     8941 cgcgctatac cagcgggaaa atgaaagcgt ttttatattg cggtaatgtt gccgatgtgg
     9001 gcgagctaca ttgttaaagc ctatgcctgg acgttattgc tggcaaaaga tggcgtggct
     9061 cagtggtttt tacaacatct tgggctggaa ccactgctga ctgcgttcct tacattacct
     9121 gcggtgggcg gaaatacgct gtcaacttcc gggctggggc gctttctggt gtttctctat
     9181 atctggttgc cgttcatgat cctgcccgtt caggcggcgc ttgagcgttt gccgccgtca
     9241 ttgttgcagg cgtcggctga tctcggcgca cgtccacgac aaacctttcg ctatgtggtg
     9301 ctgccgctgg caatcccggg tattgccgct ggctctatct ttaccttctc actcacactg
     9361 ggcgatttta tcgtcccgca gctggttggt cctccaggat attttatcgg caatatggtt
     9421 tattcccagc agggggcgat tggcaatatg ccgatggcgg cggcattcac cctggtgccg
     9481 attattctca tcgcactgta cctggcgttc gtgaaacgtc tgggagcgtt cgatgcactc
     9541 tgaacgcgca ccgtttttcc tcaaactggc ggcctggggc ggcgttgttt tcctacattt
     9601 tcccatcctg ataatcgccg cctatgcgtt taacactgaa gatgcggcgt ttagttttcc
     9661 accgcagggc ctgacgctgc gctggtttag cgtggcagca cagcgtagtg atattcttga
     9721 tgccgtgaca ctgtcactta aagtggcggc gctggcgaca ttaattgcgc tggtgttagg
     9781 gacgctggca gctgccgcgc tgtggcgacg agactttttc ggcaaaaacg ccatttcgct
     9841 gttactgctg ctgcccattg cgctgccggg cagcgtcact ggtctggcgt tattaaccgc
     9901 ctttaaaacc atcaatctgg agccgggatt tttcaccatc gtggtcggtc atgcgacttt
     9961 ttgtgtagtt gtggtgttta acaatgtcat cgcccgtttt cgccgcacct cctggagtct
    10021 ggttgaggcg tcaatggatc ttggggccaa tggctggcaa accttccgct acgtagtgtt
    10081 gccgaatctc agttcggcgt tactggcagg aggaatgctg gcgtttgcct tgtcgttcga
    10141 tgaaatcatc gttacgacct ttacggcagg tcatgaacga acgttactgt tgtggttgct
    10201 caatcagctt gggcgaccgc gtgatgtacc ggtaactaac gtggtggcac tgctggttat
    10261 gttggtaaca accttgccga tcctgggggc ctggtggcta acccgcgaag gcgacaatgg
    10321 tcaataacca ctgatacagg aatatgctat gcaacataag ttactgatta acggagaact
    10381 ggttagcggc gaaggggaaa aacagcctgt ctataatccg gcaacggggg acgttttact
    10441 ggaaattgcc gaggcatccg cagagcaggt cgatgctgct gtgcgcgcgg cagatgcagc
    10501 atttgccgaa tgggggcaaa ccacgccgaa agtgcgtgcg gaatgtctgc tgaaactggc
    10561 tgatgttatc gaagaaaatg gtcaggtttt tgccgaactg gagtcccgta attgtggcaa
    10621 accgctgcat agtgcgttca atgatgaaat cccggcgatt gtcgatgttt ttcgcttttt
    10681 cgcgggtgcg gcgcgctgtc tgaatggtct ggcggcaggt gaatatcttg aaggtcatac
    10741 ttcgatgatc cgtcgcgatc cgttgggggt cgtggcttct atcgcaccgt ggaattatcc
    10801 gctgatgatg gccgcgtgga aacttgctcc ggcgctggcg gcagggaact gcgtagtgct
    10861 taaaccatca gaaattaccc cgctgaccgc gttgaagttg gcagagctgg cgaaagatat
    10921 cttcccggca ggcgtgatta acatactgtt tggcagaggc aaaacggtgg gtgatccgct
    10981 gaccggtcat cccaaagtgc ggatggtgtc gctgacgggc tctatcgcca ccggcgagca
    11041 catcatcagc cataccgcgt cgtccattaa gcgtactcat atggaacttg gtggcaaagc
    11101 gccagtgatt gtttttgatg atgcggatat tgaagcagtg gtcgaaggtg tacgtacatt
    11161 tggctattac aatgctggac aggattgtac tgcggcttgt cggatctacg cgcaaaaagg
    11221 catttacgat acgctggtgg aaaaactggg tgctgcggtg gcaacgttaa aatctggtgc
    11281 gccggatgac gagtctacgg agcttggacc tttaagctcg ctggcgcatc tcgaacgcgt
    11341 cagcaaggca gtagaagagg cgaaagcgac agggcacatc aaagtgatca ctggcggtga
    11401 aaagcgcaag ggtaatggct attactatgc gccgacgctg ctggctggcg cattacagga
    11461 cgatgccatc gtgcaaaaag aggtatttgg tccagtagtg agtgttacgc ccttcgacaa
    11521 cgaagaacag gtggtgaact gggcgaatga cagccagtac ggacttgcat cttcggtatg
    11581 gacgaaagat gtgggcaggg cgcatcgcgt cagcgcacgg ctgcaatatg gttgtacctg
    11641 ggtcaatacc catttcatgc tggtaagtga aatgccgcac ggtgggcaga aactttctgg
    11701 ttacggcaag gatatgtcac tttatgggct ggaggattac accgtcgtcc gccacgtcat
    11761 ggttaaacat taagttagat gttagtggct gttataggac ggccactatc gttttaggtt
    11821 ctgagtttaa tggcggttaa gtattagaat gattacatta ataactagcg taaaaattag
    11881 gctaaatgtt tgatggcgat ttttcatcct gtcacagttg attagcataa ggctttagag
    11941 tatcgttatt aaactaataa tttataagta gaagtattaa caatgaaagt tcaattgtac
    12001 aaattcacgg aagataaaaa taaaaccctt accttcagat ggacgaaaaa acactttgag
    12061 ttttgtatgg ataataaaat
//
LOCUS       DAKISA010000123        11972 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.123, whole genome shotgun sequence.
ACCESSION   DAKISA010000123 DAKISA010000000
VERSION     DAKISA010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11972
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.123"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(252..2123)
                     /gene="ppiD"
                     /locus_tag="OVW45_003610"
     CDS             complement(252..2123)
                     /gene="ppiD"
                     /locus_tag="OVW45_003610"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCV5527964.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRNPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(2315..2587)
                     /gene="hupB"
                     /locus_tag="OVW45_003611"
     CDS             complement(2315..2587)
                     /gene="hupB"
                     /locus_tag="OVW45_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCV5527965.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(2796..5150)
                     /gene="lon"
                     /locus_tag="OVW45_003612"
     CDS             complement(2796..5150)
                     /gene="lon"
                     /locus_tag="OVW45_003612"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCV5527966.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVCARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(5338..6612)
                     /gene="clpX"
                     /locus_tag="OVW45_003613"
     CDS             complement(5338..6612)
                     /gene="clpX"
                     /locus_tag="OVW45_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCV5527967.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(6738..7361)
                     /gene="clpP"
                     /locus_tag="OVW45_003614"
     CDS             complement(6738..7361)
                     /gene="clpP"
                     /locus_tag="OVW45_003614"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCV5527968.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(7607..8905)
                     /gene="tig"
                     /locus_tag="OVW45_003615"
     CDS             complement(7607..8905)
                     /gene="tig"
                     /locus_tag="OVW45_003615"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCV5527969.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(9249..9566)
                     /gene="bolA"
                     /locus_tag="OVW45_003616"
     CDS             complement(9249..9566)
                     /gene="bolA"
                     /locus_tag="OVW45_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCV5527970.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            9871..10449
                     /locus_tag="OVW45_003617"
     CDS             9871..10449
                     /locus_tag="OVW45_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCV5527971.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            10493..11968
                     /gene="ampG"
                     /locus_tag="OVW45_003618"
     CDS             10493..11968
                     /gene="ampG"
                     /locus_tag="OVW45_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCV5527972.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPTGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         2637 a         3270 c         2864 g         3201 t
ORIGIN      
        1 gccgtcggtt tcgccacaga agctgccgcc agcgcgctat gagacattcc ggcacaggcc
       61 agggacaggg taatgagcag tgctttaatt ccgtgtttca tactgttttc tccttgtttg
      121 ttaacagtgg aatcacctta acgggagtga aaacagtaaa caaatggcaa tgttcagaaa
      181 tggaaaaggc cgcgaaagcg accttttctt gttacagctg caacgcattt ttttgcgagg
      241 cggcttcagg attattgctg ttccagcgca tcgccaattt tgattttcgc ctctttacgc
      301 aggttactca tcagagcttc aaagacgatt tgtgcgttgt tctgggtgat accctgcacc
      361 atcgcttttt tctgatcttc cggcattgaa ccttgtttca cttcatccag cgccagcaga
      421 accacattac cttgcatatc ggtcgccata ccgtagctcg gtttgtcttt cgctggcagt
      481 ggcagtgcaa acgccgcctg gctaatcggg ttacgaccgg aacggcttaa ggttttcggc
      541 tcgccaaatt tcagaccggc agcctgcata gcttccgccc ctttgccggc tttcaaatca
      601 accagcagtt tctcagcatc cactttcgcc tgttgttcag ctttgttgtg ctgaaccagt
      661 gccttaactt gttcctgaac atctgccaac ggtttcaccg cttccggttt gtgctcgctg
      721 atgcgcagca cgaatgcgcg gtcgccgtct acggtgatga tgtcagagtt gatgcccggc
      781 gcgccgtttt cacctaccag accgccgtta aagatagcgt cggcaaccgg cttgaagttc
      841 aactcttccg gcaggttatc tttgctgaac caacccgtct gagtggcttt aacgccggca
      901 gcttgctctg caccggccag agactcggtg tcgttgcttg ccgcatcgct cactttctgc
      961 tgcagcgcat agtacgcatc gagggctttt tcgtgtttca ctttcgccgc aatgtcgtca
     1021 cgtacttcgt ctaacgattt cactttcgct ggctgaatgt cgtccagacg tacaatcagg
     1081 aaaccgaccg aagatttgat gacaccagac agttggcctt tttctttcag accagcattt
     1141 ttcagttcgt cggggatagt ggcatcttct aaccaaccca tatcgccgcc gttacgggca
     1201 gagataatat cggcagattt ttctttggct aatgcagcaa aatcaccgcc tttattcagc
     1261 tcatcaagta ccgctttcgc ttcatcttca gttttggtct ggatgatgct gtagcgggta
     1321 cgctgcggct gggtgaattg atcctgatgc tgatcgtagt agctctggat atccgcatcg
     1381 ctaaccggtt gctgcatcgt tgcggcatcc agcttgatgt aactcacgcg gaattgttcc
     1441 ggcgtcatga aattgttttt gttttgttcg tagtagctgg caatctcctg ttcggtcaca
     1501 ggctgcttcg ccgccagcgc gttaacatcg atagtcgcct cacgcaccac gcgttgttgc
     1561 gcgaccagtg ccgccagctc gtcggtttca cctttcagca taaaatcggt accggcaaca
     1621 ccgttaatca gctgttgggt agtgagctgg ttacgcagcg cctgggcgta ctgatcggcg
     1681 gtcatcccca tctggttgag gataccgtta tagcggctgt tatcaaattt gccgtcaacc
     1741 tggaaggctg gggtcgcgaa aatcgcctgt ttaacctgct catcactgat acccagtttc
     1801 agctcacgag cgtactgatc caacagcgcc tcgtcgatca gacgattcag cacctgttga
     1861 cgcagggttt tcatatagcc ttcgttcgct gccagctcgg agtattgatc gcccagctgt
     1921 tgctgcatgc gattacgctc gctgttaaag gcgttctcga actgcccacg gctgatttcc
     1981 tggtcattca cttttgcggc gtagttattg cctccgccaa tcaggtaacc actcacgccg
     2041 gtcaatatga acgacacgat aatgataccg aaaataatct tgagcacgag actgtttgca
     2101 gccgtgcgta agctgtccat catggtgtaa caacactccg ctgtagatga ctgtgtacct
     2161 cacgcaacgt atcgatgcgc gcaaaggcct attgtgacaa gaaacggggg caattgtcag
     2221 cccacaagac acagaaagtc accgaataca aataaaaaag gcacatcagt agatgcgccc
     2281 ttgaacttcg tcacatcccc actggggaca acgcttagtt taccgcgtct ttcagtgctt
     2341 tacctgcacg gaagctcggt actttagcgg cagcgatggt gatctcttta ccggtctgcg
     2401 ggttgcggcc agtacgggca gcacgctctt taacggcaaa agtaccaaaa cctaccagtg
     2461 ctacatcatc cccttctttc agagattcag ttacggaagc aataatagca tctaacgcac
     2521 ggccagccgc agctttagag atatcagccc ctgcagcaat cttgtcgatc aattgagatt
     2581 tattcactct tctcttcctc tttataattt atatcgcact tgaatccttc aaggtacgaa
     2641 cgcgcagcag ttatatcagg ccagccatcc ccttacaaca ccccttaata agggcaagcc
     2701 cgatccgcca tctaacttag cgagacaaaa aaaggctggc aagcccgaat tagcctgcca
     2761 gccctgtttt tattagtgca ttttgcgcga ggtcactatt ttgcagtcac aacctgcatg
     2821 ccagacggtt cattttgcag cgccagagtc agaacttcct caatgcgctt cacaggatga
     2881 atgtccagat cggcaattac gttgtcagga atctcttcca gatcgcgttt attttcgaac
     2941 ggaattagca ctgttttaat cccgccgcga tgcgctgcca ggagtttttc tttcaaacca
     3001 ccgatcggca gtacctgacc acgcagagtg atctcaccgg tcattgccac atcggcacga
     3061 accgggttac cggtcaggca agaaaccagc gcggtgcaca tagcaatacc ggcactcgga
     3121 ccatctttcg gcgtcgcacc ttccggtacg tggacgtgga tgtcacgttt ttcgtaaaaa
     3181 tcagggttga tccccagttt ttccgcacgc gcacaaacca ccgttaacgc cgcctgaata
     3241 gactcctgca tcacttcgcc gagcgaaccg gtataggtca gtttgccttt acccggaaca
     3301 catgcggttt caatggtcag caagtcaccg cccacttccg tccacgccag accggttacc
     3361 tgaccgacac ggttttcgtt atcagcgcga ccatagtcga aacgctgaac accgaggtag
     3421 tcatgcaggt tatcgccgtt aatttcgata tgttttaatg acttatcgag cagtaactgc
     3481 ttaaccgctt tgcggcacag tttggagatt tcacgctcca gaccacgcac gcccgcctca
     3541 cgggtgtagt aacgaataat gccgataatg gcgctatcgt cgacggtcag ctcacctttt
     3601 ttcagtgcat tacgttcaat ctgcttcggc agcaggtgac gtttggcgat gttcagtttt
     3661 tcatcttcgg tataaccgga gaggcgaatc acttccatac gatccagcag cggtgccgga
     3721 atgttcatgg agttcgacgt cgcgacaaac atcacgtcgc tgagatcgta atccacttcc
     3781 aggtagtggt cgctgaacgc tacgttctgc tctggatcca gcacttcaag cagtgcagag
     3841 gccggatcgc ctcgcatgtc agaagacatt ttgtcgatct catcgagcag gaacagcggg
     3901 tttttcacgc ccactttcgc cattttctgg atcagtttac ccggcataga accgatgtaa
     3961 gtacggcggt gaccacggat ttccgcttca tcacgcacgc cgcccagcgc catacggaca
     4021 tatttacgcc cggtggcttt ggcaatggac tgaccaagag aggttttacc tacccccggc
     4081 ggccctacca ggcagaggat cggtcccttg attttgttga cacggctttg aaccgcaagg
     4141 tactcaagga tgcgatcttt cacgcgctcc agaccataat ggtcggtatc aaggatttcc
     4201 tgcgcctgac gcaggtcttt tttgaccttg ctgcgcgcat tccacggtac ctgtaccatc
     4261 cagtcgatat aaccacgcac tacggtcgct tctgccgaca tcggagacat cattttcagc
     4321 ttctgcaact ctgcttccgc tttctctttt gcctctttcg gcatcttcgc cgcgtcgatt
     4381 ttgcgcttca gggcttcgtt ttcgtccggc gcgtcgtcca tttcaccgag ttctttctga
     4441 atagctttca tttgctcgtt cagatagtac tcacgctggg atttctccat ctgcttttta
     4501 acgcggttgc gaatgcgttt ctcaacctgc agcagatcga tttccgattc catcattgcc
     4561 atcagatatt ccagacgttc gttaacgtcg gacatctcca gaacggactg tttgtcagcc
     4621 agtttcagcg gcatatgtgc agcaatggta tccgccagac gcgccggatc gtcgatgcta
     4681 ttcagcgacg tcagcacttc tggtgggatt tttttgttca gcttgatgta gccttcgaac
     4741 tggctgattg cagtacgcac cagcacttcc tgttcccgct catcaatggt cggcgactcc
     4801 agatactccg ccttcgcaga aaagtgttcg ccattgtcag agagcgcaga aatacgcgcg
     4861 cgctgtaacc cctcgaccag cactttgacg gtgccgtcag gcagtttcag catctgcaat
     4921 atagaggcca cggtcccgac ggtgaaaaga tcgtttacac ccggctcatc cgttgaagct
     4981 tctttctgcg cgaccagcat aattttttta tcatggtcca tcgccgcttc cagacaacgg
     5041 atagattttt cccgcccgac aaataagggg atgaccatgt gcggataaac caccacatcg
     5101 cgcagcggca atacggggat ttcaatgcgt tcagaacgct caggattcat agagctctct
     5161 cttagtttaa tttccgccag gtaatcagat gacacgactg tgcttcacgc catctattaa
     5221 catgtacgtc agtatatggg gatgtttccc ccacattcaa cgccgagaat agaggaaaaa
     5281 ttaaagggga gataaaatcc cccctttttg gttaactaat tgtatgggaa tggttaatta
     5341 ttcaccagat gcctgttgcg cttccggctt accataaatc agcaacggtt tgctttgacc
     5401 atcaattacc gactcgtcga taaccacttt ttcgacgtct tccatggacg gcagatcgta
     5461 catggtatcg agcagtgcgg cttctacgat ggaacgcagg ccacgggcac cggttttacg
     5521 cgccatcgct ttcttagcga tagcatccag cgcctcgtca cggaattcca gatccacgcc
     5581 ttccagatta aacagcgcct gatactgctt ggtcagggcg tttttcggct ctttgaggat
     5641 ctgaatcaga gcttcttcgc tcagttcatt caacgttgct acaaccggca gacgaccaat
     5701 gaactcaggg ataagaccaa acttgatcag atcttccggt tcaacctgcg ccagcagctc
     5761 gccttcgctt gctttgtcgg acttcgcttt taccgtcgcg ccaaaaccaa tgccggagcc
     5821 ggtttctaca cggtgggaaa tcactttatc cagaccggca aacgcaccgc cacaaataaa
     5881 caggatctta gaggtatcaa cctgcaagaa ttcctgctgc ggatgtttac gcccaccttg
     5941 cggtggaaca gcagctaccg taccttcgat cagtttcaac agtgcctgct gtacgccttc
     6001 accggaaacg tctcgggtaa tggacgggtt gtctgactta cgagaaatct tgtcgatttc
     6061 atcgatgtag acaatcccgc gctgcgcttt ctgtacgtcg taatcgcact tttgcaacag
     6121 cttctgaatg atgttttcaa cgtcttcgcc cacataaccg gcttcggtca gcgtggttgc
     6181 gtcggccatg gtgaacggga cgtccagcag gcgcgccagc gtttcagcca gcagcgtttt
     6241 accggaaccg gtcggaccga tcagcagaat gttacttttg cccaactcga caccattgct
     6301 ggtatcgccg ttgcgcagac gtttgtaatg gttgtatacc gcgaccgcca gcactttttt
     6361 cgcctgttcc tggccgataa cgtaatcgtc caggtggttg cgaatttcat gcggcgtcgg
     6421 tagcgcactg cgttcacgat gcggtgcaac ttctttaatc tcttcgcgaa tgatgtcgtt
     6481 acataaatca acacattcgt cgcagatata cacggatgga ccggcaatca gcttgcgcac
     6541 ttcatgctgg cttttgccgc aaaaagagca atacagcaat ttgcctgagc catctttgcg
     6601 tttatctgtc atgggtcaaa acctcttctt tgttctttgt gccgcacacg acgacgcaaa
     6661 tgccattctc aggcgcaagc cgcttacagc gttgtgccgc cctggataag tatagcggca
     6721 cagttgcgcc tctggcatca attacgatgg gtcagaatcg aatcgaccag accgtattcc
     6781 accgcttcag gggcggaaag gaagcgatcg cgctcggtat cacgttcaat ctgttctaat
     6841 gattgacccg tatgaagcgc cataagttca ttcatgcgcc ctttaacttt cagaatttca
     6901 cgggcatgaa tttcgatatc ggtcgcctgg ccctggtagc cgcccaacgg ttggtgaatc
     6961 atcacgcgcg aattcggcag gcaaaaacgt ttaccttttg cccctgcggt cagcaagaaa
     7021 gcgcccatcg aggccgcctg gcccatacag atggtgctga catcaggctt gataaactgc
     7081 atggtgtcat agatagacat cccggcagtg atcaccccgc ctggggagtt aatgtacaga
     7141 tagatatctt tttctgggtt ttccgcttct aggaacagca tctgcgccac aatcaggttc
     7201 gccatgtggt cttcaacctg gccagtcaga aaaatgacgc gttccttaag tagacgagaa
     7261 tagatatcaa aagagcgctc accgcgtgag gtctgttcaa tgaccatcgg caccagcgcc
     7321 atatggggtg caaagttatc tcgttcgccg ctgtatgaca tttccgtctc ctggataaaa
     7381 ttgaaaaaac ctgctgtacc gattgtaacc ttatggacgg attatcagct agtccctgtg
     7441 ttatgggtac ggcatcacca tatttcaagc ataacaatct tttgttgtta cgctaacacc
     7501 gaaagaggct tttcgcacgg ttccatgcaa aattcatgac aaaaaaaagc ccaccacctg
     7561 gcggtgacgg gcctttgtgc gaatttagcg cgttatgctg cgtaaattac gcctgctggt
     7621 tcatcagctc gttgaaagtg gtttcttttt cagtcacttt cgctttcgcc agtacagctt
     7681 caacagcctg ttcttccaga gcaacattgc gcatgttgtc catcagttct ttgtttttgc
     7741 tgtagaactc gataacttct ttcggatctt cgtacgcaga agccatctct tcgatcaggc
     7801 ctttcacgcg ctcttcgtca gctttcagct cgttggtgcg gataacttcg cccagcagca
     7861 ggccaacaac tacgcggcgt ttagcctgtt cttcgaacag ttcgcgcggc agttccagag
     7921 cttgtttttc gttgccaccg aaacgctgtg cagcctggcg acgcagaacg tcgatttcgc
     7981 tgtcgatcag cgcagccggt acgtcgatgt cgttagcttt taccagacct tcgatcgcct
     8041 gagacttaac gcggttacgg atggcgctct tcagctcgcg ctccatgttt ttacgcactt
     8101 cagcgcgcag accttctacg gaaccatctt caacgccgaa acgtttgatg aattctgcag
     8161 tcagttccgg cagttcacgc tcttcaactt tcttcaggtt gatagcgaat ttcgctgctt
     8221 tacctttcag attttctgcg tggtattctt ccgggaaggt cacgtcgatg gtgaactctt
     8281 cgccagcttt gtggcctttg ataccgtctt caaagcccgg gatcatacga ccctggccca
     8341 tcgccagtac gaaatcagac gctttaccgc cttcgaactc ttcgccgtct acagaaccgg
     8401 tgaagtcgat ggttacgcgg tcttctgctt caacggcgcc gtctttttct ttccaggtcg
     8461 cctgctgttt acgcagagta tccagcatgc cgtcaacgtc agcgtcggtc acttcaacga
     8521 tcggtttttc aacttcgatc gcttccagac cctgcagttc aacttccgga taaacttcaa
     8581 actctacaga gtaggtgaag tcttcaccca gcttgtattc gcccggaaca taagtcggtg
     8641 cgccagccgg attgattttt tctttaatga tggcgtcaat gaagttacgg ctcatcaggt
     8701 cacccagaac gtcctggcgt acagacgcgc cataacgctg agcaacgata ttcattggca
     8761 ctttgccttt gcggaagccg tcaatacgta cttttttcgc aacgttgacc agctcgcttt
     8821 taacagcggt ctcgatgctg tcagcagcga tagtaatcgt tacacggcgg ccaaggcctt
     8881 gagtggtttc aactgaaact tgcatcttgt tacctcaaaa aatcacagtg ctcggtcaac
     8941 tcttaccccg caagcacagg ttgttggctt cgcggaaccg ggatgttctt ataatcaatc
     9001 acattccctg ttaccagaat catcccgaaa gcattcaaat aggacgcggc attatagcgg
     9061 catcactttt atgagtcgag aacggttatt gcgcgctgtc gcgtattttt tcacaattcc
     9121 cgggcaattt tcgtctgaat gctgggcaaa aagcgcaaaa tccagacaaa acaaaacggc
     9181 ccgcaggccg tttttcataa aatctttagc aacatactgg aaaagcgccg acagttgcaa
     9241 atgcgttttt acgcgatgct tcctgctcca cgacagggag gagaggcaaa gacggtgtcc
     9301 tgcaaccctt cccactcctt aatagtgtaa gtatgcagag ccagcgcatg aacggtagta
     9361 gagagttcct ccgctaaagt actgtaaatc attcgatgac gattcagaaa acgttcaccc
     9421 gtaaaacgat cgctgaccag cacaacttta aaatggcttt cagagccggc tgggacattg
     9481 tgacgatagc tttcatccac tacttcgagg aatacgggtt ggaacgccgc ccttaatttt
     9541 tcttctatcc gctcacgtat catcatgaaa tatctcctcc gacaacgctg agatgtcatc
     9601 catcccttta aatactagcc gcttttaccg tttccattgc agcttaacaa caaatattta
     9661 ggtttcgtcc tggtttttcc gtcaagcgta tgaagtttac tgatttaaag attgaaacac
     9721 aggcctcgca tcagcgtttc ttttaccagg ggtgattatt tcgccaacat gatgaattta
     9781 catgccttac ccacttcccc tcgccgttgg caatgttatg atggcgggaa tttttaccaa
     9841 acacgcttaa gagtctctga gagcccgaac atgttcaaaa aaatcctctt cccgttagtt
     9901 gctctgttta tgcttgcagg atgcgcaaaa ccgccaacaa ctattgaagt ttccccgacg
     9961 attacactgc cacagcagga tccaagcctg atgggcgtca ccgtaagcat taatggtgcc
    10021 gatcagcgta ccgatcaggc gctggcaaaa gtcacccgcg ataatcaaat cgttaccctg
    10081 accgcctccc gagatctgcg tttcctgctg caagaagtgc tggaaaaaca gatgaccgcg
    10141 cgtggttaca tggttggtcc gaatggcccg gttaatctgc aaatcatcgt tagccaactg
    10201 tatgctgacg tgtcccaggg caacgtgcgc tacaacatcg cgaccaaagc ggatatcgcc
    10261 atcatcgcta ccgcgcagaa tggcaacaaa atgaccaaaa actatcgtgc cagctacaac
    10321 gttgaaggtg cgttccaggc ctccaacaaa aatatcgccg atgcggttaa cagtgtgctg
    10381 accgatacca tcgctgatat gtctcaggac accagcatcc acgaattcat caagcagaac
    10441 gcgcgttaat ttctgccctc tggcccggtg caaaccgggc ctgtagacgc ccatgtccag
    10501 tcaatattta cgtatttttc aacagccgcg ttcagccata ttgctgatcc tgggttttgc
    10561 ttccgggcta ccgctcgccc tgacatccgg taccttacag gcctggatga cggtcgagaa
    10621 tattgatctc aaaaccattg gtttcttctc tctggtaggc caggcttacg tttttaaatt
    10681 cctctggtca ccgctgatgg accgctacac gcccccattt tttgggcggc ggcgcggttg
    10741 gctgctcgcc acgcaaatcc tgttattagt cgccattgcg gcgatgggtt ttctcgaacc
    10801 tggcacccaa ctccgctgga tggcggcact ggcggtggtg atcgcttttt gctcggcctc
    10861 ccaggatatt gtcttcgatg cgtggaaaac cgatgtgctc ccggcagaag aacgtggtgc
    10921 gggcgcggca atcagcgtgc tgggttaccg tttagggatg ctggtttccg gcggcttagc
    10981 cctgtggctg gcagataaat ggctgggctg gcagggcatg tactggttga tggcggcgct
    11041 gttgatccct tgtattatcg cgacgttgct tgcaccagaa ccgaccgaca ccattcctgt
    11101 gccaaaaacg ctggaacaag cggttgttgc acctctgcga gatttctttg gtcgcaataa
    11161 tgcctggctt attttgcttc ttatcgtgct gtataagctg ggcgacgcat tcgccatgag
    11221 cctgacaacc acgtttttga ttcgcggcgt cgggtttgat gcgggtgaag taggcgtggt
    11281 taacaaaacg cttggcttac tggcgaccat tgtgggcgca ttgtacggtg ggattttgat
    11341 gcagcgcctg tcgctgttcc gggcgctgct gattttcggc attttacaag gtgcgtctaa
    11401 cgctggttac tggctgctgt cgattactga taagcatctc tacagcatgg gcgcagccgt
    11461 ctttttcgaa aacctctgtg gcgggatggg cacatcagcc tttgtcgcgc tgttaatgac
    11521 gctgtgtaat aagtcatttt ccgctactca atttgccctg ctctcagcgc tttccgctgt
    11581 aggacgagtt tatgtcggcc ccgtggcggg ttggtttgtt gaagcacacg gctggtcgac
    11641 attctatcta ttctccgtcg ccgctgccgt accggggctt attttgctgc tggtttgccg
    11701 ccagacgctt gaatatacac gagtaaatga caactttatc tcccgtaccg aatatccgac
    11761 aggttatgcc tttgccatgt ggacactggc ggcgggcgtc agcctgttgg ccgtgtggtt
    11821 actgctgttg acgatggacg cgctggattt gacgcacttc tctttcctgc ctgctctgct
    11881 ggaagtcggg gttttagtcg ccctttctgg cgtcgtgctt ggtggtttgc tggattatct
    11941 ggctctacga aaaacgcatc tgacgtaatc tg
//
LOCUS       DAKISA010000124        11808 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.124, whole genome shotgun sequence.
ACCESSION   DAKISA010000124 DAKISA010000000
VERSION     DAKISA010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11808
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.124"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..488
                     /locus_tag="OVW45_003619"
     CDS             <1..488
                     /locus_tag="OVW45_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCV5527973.1"
                     /translation="NVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHH
                     RKSEITFYHREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGARWNAAVKRAGIRRR
                     NPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARL
                     S"
     gene            540..1475
                     /gene="ttcA"
                     /locus_tag="OVW45_003620"
     CDS             540..1475
                     /gene="ttcA"
                     /locus_tag="OVW45_003620"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCV5527974.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1604..2977)
                     /gene="dbpA"
                     /locus_tag="OVW45_003621"
     CDS             complement(1604..2977)
                     /gene="dbpA"
                     /locus_tag="OVW45_003621"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCV5527975.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     SRVRLLK"
     gene            complement(3455..4438)
                     /gene="zntB"
                     /locus_tag="OVW45_003622"
     CDS             complement(3455..4438)
                     /gene="zntB"
                     /locus_tag="OVW45_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCV5527976.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            4693..5925
                     /gene="dgcM"
                     /locus_tag="OVW45_003623"
     CDS             4693..5925
                     /gene="dgcM"
                     /locus_tag="OVW45_003623"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HCV5527977.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALNCRLVLRKLTEAEPVI
                     IFEGIEASATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNHVLAA"
     gene            complement(5946..6509)
                     /gene="smrA"
                     /locus_tag="OVW45_003624"
     CDS             complement(5946..6509)
                     /gene="smrA"
                     /locus_tag="OVW45_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HCV5527978.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(6839..7747)
                     /locus_tag="OVW45_003625"
     CDS             complement(6839..7747)
                     /locus_tag="OVW45_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCV5527979.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            7923..9233
                     /gene="abgA"
                     /locus_tag="OVW45_003626"
     CDS             7923..9233
                     /gene="abgA"
                     /locus_tag="OVW45_003626"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HCV5527980.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            9233..10678
                     /gene="abgB"
                     /locus_tag="OVW45_003627"
     CDS             9233..10678
                     /gene="abgB"
                     /locus_tag="OVW45_003627"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HCV5527981.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLRGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            10715..>11808
                     /locus_tag="OVW45_003628"
     CDS             10715..>11808
                     /locus_tag="OVW45_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HCV5527982.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVLHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWSLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDATLVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPQ
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSN"
BASE COUNT         3007 a         3001 c         2925 g         2875 t
ORIGIN      
        1 tgaatgtcag aagaaacctg acgatacttg atatgttcgg tcccccaaaa acaaatgccg
       61 ggatccggac ggtaacatta ctgcagccgg ctcttgaagc actgaaggag caatacaaac
      121 tgaccgggca tcatcgcaaa agcgaaatca ctttttatca tcgggagtac ggcagaactg
      181 aaaagcaaaa actgcatttt gttttcatgc ccagggtgtg taacgaaaaa cagaaacctt
      241 attactcggt aagcagtttg ggtgcaagat ggaatgcagc agtaaaacgt gctggtattc
      301 gccgccgtaa tccgtaccat acgcgacata cttttgcctg ctggctgttg acggcaggag
      361 cgaacccggc atttatagcc agccagatgg ggcatgaaac tgcgcaaatg gtgtatgaaa
      421 tttacggtat gtggattgat gacatgaacg acgaacagat agccatgttg aatgcgcggt
      481 tatcgtagtt gcaaagtttg cccccaattt gccccattta gtaccagaga actgaaataa
      541 tgcaagaaaa tcaacaaatt acaaagaaag aacaatacaa cctgaacaaa ttacaaaaac
      601 gtctgcgtcg taacgtgggc gaagccattg ctgacttcaa tatgattgaa gaaggcgatc
      661 gcatcatggt ttgcctctcc gggggtaaag acagctatac catgctggag attctgcgca
      721 atttgcagca aagcgcgcca atcaattttt cgctggtggc tgttaacctc gatcaaaagc
      781 aaccgggctt cccggaacac gttctgcccg agtatcttga aaagctgggc gttgagtaca
      841 agattgtcga agagaacact tacggtatcg tgaaagagaa gattccagag ggcaaaacca
      901 cttgctcact gtgttctcgc cttcgtcgcg gtatccttta tcgtaccgca acggaactgg
      961 gggcgacgaa gatcgcgctg ggtcaccatc gtgacgatat cctgcagacg ctgttcttaa
     1021 atatgttcta cggcggcaag atgaaaggta tgcctccgaa actgatgagc gatgatggca
     1081 aacatatcgt tattcgtccg ctggcctact gccgcgagaa agatattcag cgatttgccg
     1141 atgcaaaagc gttcccgatt attccgtgca acctgtgcgg ttcacagcct aacctgcaac
     1201 gtcaggtgat tgctgacatg ttgcgtgact gggataaacg ttatccaggg cgtatcgaga
     1261 cgatgttcag cgcgatgcag aatgtggtgc cgtcgcatct gtgcgatacc aacctgttcg
     1321 atttcaaagg catcacccac ggttctgaag tggttaacgg gggtgatctg gcgtttgatc
     1381 gcgaagagat cccactacaa ccggcgggct ggcagccaga agaagatgaa aatcagttgg
     1441 atgagttacg gctgaatgtg gttgaagtga aataaccagg atggcgcccg atgcgcaagc
     1501 gtatcgggct actcttatgg aggccggata agacgcggcc agcgtcgcat ccggcaatcc
     1561 cgaataagat gtttactctt gcacccggca attcaacatt tcattatttt aataaccgca
     1621 cccggctcgt ttttccttta atcttcccgc cctgtaactg tttccatgct ttatgagcaa
     1681 cagcctgacg gaccgcgaca tagacatgcg ccggatgcac ggcgattttg ccaatatctg
     1741 cgccatcaag cccgatatct cctgtcagtg cacctaatac atcacccggg cgcattttgg
     1801 cttttttccc accatcgata cacaacgttg ccatttctgc ttccagcggc acaatggaac
     1861 tattagctgg cggcgtttgc cagttaagtt ttatctgcaa catgtcagaa atgatattgg
     1921 cccgctgtgc ttcttccgga gcacagaaac tgatcgccag accgctattt cctgcacgag
     1981 ctgtacggcc gatgcgatgt acatgaactt cagggtccca cgccagctca aagttcacca
     2041 ccagctcaag cgatttaata tccagaccac gcgcagcaac atcagtcgca accagtacac
     2101 gggcgctacc gttagcaaaa cgtaccaggg tctgatcgcg atcgcgttgc tccaaatcgc
     2161 cgtgtaatga caatgcactt tgccctactt cattcagcgc gtcgcagaca gcctggcaat
     2221 cttttttggt attgcaaaac accacgcaag aggatggctg atgcaagctt aataaccgtt
     2281 gcaacagagg aattttgcct ttgctggatg tctcataaaa ttgttgttca atgggtggca
     2341 aagcatctgt tgagtcaatt tcaatcgcca aaggatcgcg ttgcactcgt ccgctgatta
     2401 cggcaatggc ttccggccag gttgccgaaa acagaagcgt ctgtcgagat gcaggcgcaa
     2461 aacggatgac atcatcaatg gcatcgctaa atcccatatc cagcatgcgg tcggcctcat
     2521 ccatcaccaa agtattcagc gcatccagtg ataccgtgcc tttttgcaag tgatccagca
     2581 aacgacccgg cgttgccacg ataatatgcg gcgcatgttg caacgaatca cgctgcatac
     2641 cgaacggttg accaccgcac aacgtcaaaa ttttggtatt tggcagaaaa cgcgccagcc
     2701 gacgcaattc acctgccacc tgatccgcca gttcacgcgt aggacacagc actaaagcct
     2761 gggtttgaaa tagcgacgca tcaatttgct gtaacaagcc gaggccaaaa gccgccgttt
     2821 tgccgctgcc ggttttcgcc tgcacgcgaa catcttttcc ggcaaggatc gccggaagcg
     2881 cggcggcctg caccggcgtc atagttaaat aacccaactc attaaggttc gtgagttggg
     2941 cgggaggcaa aacattcagg gtagaaaaag cggtcacaat ctattctcgt ggtcatcgac
     3001 gcaaagttag caggcgcgta tcctcgcaga tctacgctca cgatgcgaca atttaatcgg
     3061 ttcttcatcg ggtggtgggt caggcatggg ttgcgggcga gggatcggat cgggcactgg
     3121 aacaggatcg ccaggaatcg gttcagggac aggaatttgc aaataaataa gtgtcgtcat
     3181 atttccctcc ggtcattggg tggactctta aagggtagac gctgataaat aacaggcaaa
     3241 aaaaagccga ctcatcaaag tcggcgtcgt acgaatcaat tgtgctatgc agtaattcaa
     3301 aaaaggaagt aagacaatat ggagcgcaac gcccatcgct tgacgttgca ttcacctgca
     3361 agggagatat tgccctgaat gggtagagag tttattgact tcgctcaaac tttgcggcgt
     3421 ttttgtatac agacagccgg aaaaattgct tttgttacaa ccatttacta cggtgcaacc
     3481 ataaagcaac accaccaata agaacaacta acagaataca aaaaattgaa aatccgaatt
     3541 gccacccgcc gccagggatc ccaccaaggt tgacgccaaa taacccggtc agaaaggtac
     3601 tgggtaaaaa gaccattgcc atcaacgaca ttgtataggt acgacgagct aaattttcct
     3661 gcatcacctg agcgatttca tccgccatca cgccagtccg tgctatacag gcgtcgattt
     3721 cgtcaaggcc gcgcccaagg cgatcggcaa tatcctgcat ccgacggcgt tggtcatcgc
     3781 tcatccacgg caaacgttca ctggcaagac gagcataaac atcacgttgc ggtgccatat
     3841 agcgacgcat cacaattaat tgtttgcgca gcagagccag gaatccacgc ggtggaattt
     3901 gctgatcaag gagattatct tcaaggtcga taattttatc gtgcagctgc tcgataaatt
     3961 cgctggaatg atcggtcaac gcatcgcaca catccaccag ccaccctccg caatcggtcg
     4021 gacccgtgcc ctcttccaga tcgctcacca catcgtccag cgccagcact ttgcgttgtc
     4081 gggtcgaaac aattaaccgc ccgtccatat atacacgcat ggcgaccagt tgatcggggc
     4141 gttcatcggt gctgccgttt atacagcgca atgtaatcag cgtgccttca ccgagacggc
     4201 tgactcgggg ccgcgtgctc tcgcccgcca gcgcatcacg tacgttattg ggaagcagcg
     4261 gtgttgtcgc cagccattgg gcgctatcat ggtgtacata attaaggtgg agccagcagg
     4321 gatgcgcttc atcaatcaca tctgtatttt ccagcggttt aacgccgcct ctaccatcca
     4381 gcatccaggc aaatactgca tccgggacat taacgtccga tcccttaatc gcttccacag
     4441 tgcctccatc atcaacgcat tattttgtag tctagccttc tggccctgtt acgcaacatc
     4501 tcatcacccc attaccctga aatgattaat aaaattctgt ctaaattgaa tacaaaaagc
     4561 aaaatgcttt tccgtataca aaccgtgtga agtgttaaat agcgtctatc attatcagaa
     4621 ttatctgatc atatgacgtg gcttttttgc gatcggatag caacaaaaat tgataaaaat
     4681 aacgggatct caatgattac gcacaacttc aataccctgg acttactcac cagtcctgtc
     4741 tggatcgttt cgccctttga ggaacagtta atttatgcca atagcgcggc gcgactgttg
     4801 atgcaagacc tcacgtttag tcagctacga accggtccct attccgtctc ctcacaaaaa
     4861 gaactgccga aatacctctc cgatctgcaa aaccaacacg atattatcga aatcctcact
     4921 gttcagcgta aagaagagga aacagcatta aactgtcggc ttgttttgcg aaagctgaca
     4981 gaagcagaac cggtgattat tttcgaaggt atcgaagcgt cggcaacgct gggtttaaaa
     5041 gccagtcgct cggcaaatta tcagcgcaaa aaacaaggtt tttatgcgcg cttttttctg
     5101 actaactctg caccaatgtt gttgattgac ccctcacgag atggccaaat cgtcgatgct
     5161 aacctcgccg cgctcaattt ctatggttat aaccatgaaa cgatgtgcca gaaacatacc
     5221 tgggaaataa atatgctcgg gcgtcgcgtc atgcctatca tgcatgaaat ttcgcattta
     5281 cccggtggtc ataaacctct gaattttatt cataagctgg cggatggttc gactcgtcat
     5341 gtgcagacct acgccgggcc gattgaaatt tatggcgaca agctcatgtt atgtattgta
     5401 catgatatta ctgagcaaaa acggctggag gagcagctgg aacatgctgc ccaccatgac
     5461 gcgatgaccg gattactgaa tcggcgacag ttttatcaca ttaccgagcc aggccaaatg
     5521 cagcatctcg ccatcgccca ggattacagc ttgttgctca tcgacaccga tcgttttaaa
     5581 cacattaacg atctctatgg gcattctaaa ggtgatgagg tgttatgcgc cctcgcccgc
     5641 accctcgaaa gttgcgctcg caaaggcgat ttggtgtttc gttggggagg cgaagagttt
     5701 gtcttattgc taccaagaac cccactggat accgcgcttt cgctggctga aactatccgc
     5761 gtaagcgtgg caaaagtgag tatttcgggc ttaccacgct ttaccgtcag cattggtgtg
     5821 gcgcatcacg aaggaaatga aagcatcgat gaactgttta aacgcgttga tgatgctttg
     5881 tatcgggcga aaaatgatgg acgcaaccac gtgctggcgg cataagccgc ggatgcgtct
     5941 cgagatcaac gactgcgctt agcgtggcgc tcccagtttt cttgcttcgc ctgcgccgtt
     6001 ttacgtagtg cgacgtaaca cgccccgctg ccgccatgat gcggtagcgc agtgcaatat
     6061 gcctgaacat catcaaattc ggtcagccag cgcgccacat agctgcggac aatattggca
     6121 tgcgatttat catcccgtcc tttgccatga ataatcagca cgttacgcaa accatcagcc
     6181 atggcttgtt gaatgaaact gaacatcatt ttgcggcact cttccaccgg ctggcgcaaa
     6241 agattcaggc tcgcctgttg cggatattta ccactgcgca gcttatccag caccccatgt
     6301 tgcaaccctt cccgccgaaa ctccagcggc tgacttagtg ggatgatgtc gagaaatccg
     6361 gtggtgagga aattatcaag ctgcagcgtg tcgatacgct gcggcgcacg ttggttacgc
     6421 gttggatgcc agtggacatc ggtagcacgt ttcagcggct ggacatcttc catggcgtca
     6481 agaaacagcg atttgtcgtc aaggttcatg ttacatcctc ccgcaattaa gagcgcgata
     6541 tgataaccag accgggtcgg tccaacaacg tattacccaa aattccagta ataagttcca
     6601 aatattgccg atattttaag caaaatgctt atgcatgatt attcattcac gatattaata
     6661 atgtaactta tattttcgtg aaatctgtca ctgaagaaaa ttggcaacta aaggttaaaa
     6721 ccgttataac acagtcaccg gcgcagagga gacaatgccg gatttaagac gcggatgcac
     6781 tgctgtgtgt actgtagagt ctggcggatg tcgacagact ctattttttt atgcagtttt
     6841 aactttgcag atagccgcat tctcgccgga attgcgtgat taatgacgcg gtcagtggtg
     6901 tctggcgact atcacgccgc tgaatcaaat aataggccgc tttcggtaaa atttcgctaa
     6961 ccggcaacat caccagcccc tgtccgtgca agggatcgca gcccatttct tcaggcagta
     7021 tgctgagaaa atcgcttttt gccaccagac tgatacaggc tgagaacgtc tcgcagacta
     7081 caccgacctg tggcgtttgc gcctgatcgt caagcaattc actcaactgt ttgtagtaac
     7141 tgccgtgtgg cgtcggcatt gtccagctgt aatccagtaa ctgtttgatc gaacgggcac
     7201 caatggcggg gtgtcccggg cggcaaaaga tcgcgaattg cttttccagt aatttctcaa
     7261 aagtaaattc gtggtcgtac ggtccctgat aataggtatt gatggtgaaa tccaattctc
     7321 cctgacgcaa ttcattaatc atcgacacca gttgcccttc cataatgcgt acttttacct
     7381 gcggatgctg ctgatgaaaa cgagatatga cagctggcat cagactgcgg gaaatactgg
     7441 cccccatgcc gatattaatc tgccctgcca gttgcccttg tcgttggcga atatcctctt
     7501 gggctgcgcg cagctcttca agaataagac tggcgtgctg ataaaaactt tcaccggcat
     7561 cagttaacgt cacgccttta ctacggcgaa aaaagagttg cgccgctaat ccttcttcca
     7621 gctcctgaat agatttactc agtgccggtt gcgacatatt caacattcgg ctcgctccgc
     7681 gaatgctgcc ctgacgagcc acttcaacaa aagcccgaat ttgatgaatt tttacctgaa
     7741 aagccatgac gccaccgata accgttattt atcagaccaa agaaactggc atctacttta
     7801 atgcagatga ttgtgtcagg gtaatttatg aacggttaaa actgtgaaaa atcagttagt
     7861 gataagtaaa aactatcgct acgtgaaccg ggtcacactt tttactgatg acgggaaagg
     7921 ttatggagtc tttgaatcaa tttgttaatt cgcttgcccc aaaattatcg cactggcgac
     7981 gtgattttca tcactatgca gagtctggct gggtggaatt ccgcactgcc acccttgttg
     8041 cggaagaatt gcagcagctc ggctattcac tggcgctggg ccgcgaagta gttaatgaaa
     8101 gtagccggat gggactacct gatgaactca ctctacaacg cgaattcgag cgcgctcgtc
     8161 aacagggtgc gctagcacaa tggattgcgg tttttgaagg tggtttcact ggtatcgtcg
     8221 ccaccctgga taccggtcgc cccggtccgg tgatggcttt ccgtgtcgat atggacgcgc
     8281 tggatctcag tgaagagcag gatgtcagcc atcgccccta ccgcgacggt tttgcgtcat
     8341 gtaacgccgg aatgatgcat gcctgtggtc atgatggaca taccgccatt gggcttgggc
     8401 tggcgcatac ccttaaacaa ttcgagtccg gactacatgg cgtcatcaaa ctgatttttc
     8461 agcctgcaga ggaaggtacg cgtggcgcgc gggcgatggt cgatgcaggt gtcgtagatg
     8521 atgttgatta ttttactgcc gtgcacattg gcactggcgt acctgcgggc accgtggtgt
     8581 gcggcagtga taattttatg gcaaccacca aatttgacgc gcacttcacc ggtaccgccg
     8641 ctcacgcagg cgcaaaacca gaagacggtc acaatgcctt gttggcggca gcacaagcca
     8701 ctcttgcact gcatgcaatc gccccgcaca gcgaaggagc ttccagagta aacgtgggcg
     8761 ttatgcaggc aggaagcggt cgtaacgttg ttcctgcctc ggcgttgctg aaagtggaaa
     8821 cacgcggggc cagcgacgtc attaatcaat atgtttttga ccgtgcacaa caagcgattc
     8881 agggcgcagc aaccatgtat ggtgtcggcg ttgaaactcg tctgatgggt gcagctaccg
     8941 ccagttctcc ttcgccgcaa tgggtcgcat ggttgcaaag tcaggcggct caggtcgcgg
     9001 gggtcaatca ggccattgaa cgtgttgaag cgcctgcggg ttccgaagat gccacattaa
     9061 tgatggcccg cgtgcagcaa catcaagggc aagcctccta cgtggtgttt ggcacacagc
     9121 tggcggcagg tcatcacaac gaaaaattcg attttgacga gcaggttctc gctattgccg
     9181 tcgaaacgct ggcgcgcacc gcgctcaatt ttccctggac gcgaggtatc tgatgcagga
     9241 aatctatcgt tttatcgacg atgcgattga agccgatcgc caacgttata ccgatattgc
     9301 cgatcaaatc tgggatcatc cagaaacacg ttttgaagag ttctggtcag cggagcatct
     9361 ggcttcggct ctggaatctg caggcttcac cgttacccgc aacgtaggca atatcccaaa
     9421 tgcctttatt gcttcgtttg gtcaaggcaa accggttatc gccctgctgg gagaatatga
     9481 cgccctggca ggtttaagtc agcaagctgg ttgcgcgcaa cctacatccg cgacgcccgg
     9541 tgaaaatggt cacggttgcg gacacaattt gcggggaacc gccgcctttg ccgctgcaat
     9601 agccgtcaag aaatggctgg aacaatatgg gcaaggcggc acagtgcgct tttatggttg
     9661 tcctggcgaa gaaggcggct cgggtaaaac gttcatggtc cgcgaggggg tatttgatga
     9721 tgtggatgcg gcactcacct ggcacccgga agcctttgcc ggtatgttca atacccgcac
     9781 gctggcaaac attcaggcat catggcgctt taaagggatc gcagcacatg ccgcgaattc
     9841 ccctcatttg ggacgcagcg cccttgatgc cgtaacgttg atgaccactg gcaccaactt
     9901 cctcaacgaa catattattg aaaaagcgcg cgtacactat gccatcacaa atagcggcgg
     9961 gatctcgccc aacgtggtcc aggcgcaggc agaagtgctt tatcttatcc gcgcccccga
    10021 aatgaccgac gtgcagcata tttatgatcg ggtcgccaaa atcgccgaag gtgcggcatt
    10081 gatgaccgaa accacggttg aatgccgctt cgacaaagcc tgttccagtt atctcccgaa
    10141 tcgcacctta gaaaatgcca tgtaccatgc cctatcccat tttggtaccc cggaatggaa
    10201 ctccgaagaa ctggcttttg cgaaacaaat tcaggctacg ctcaccccca acgatcggca
    10261 aaacagtctg aataatatcg ccgcaaccgg tggcgaaaac ggcaaggctt ttgcactacg
    10321 tcatcgtgaa acggtactgg cgaatgaggt cgctccatat gccgccaccg ataacgtgct
    10381 tgcggcatcg actgatgtcg gcgacgttag ttggaaactg cctgttgccc agtgtttcag
    10441 cccctgtttt gccgtcggta caccgctaca tacgtggcaa ctggttagcc aggggcgaac
    10501 atctattgct cataaaggaa tgctgctggc ggcgaaaact atggcagcaa ccacagtcaa
    10561 tctcttcatt gattcagggc tattgcaaga atgccaacaa gagcatcagc aagtaacgga
    10621 cacgcaaccg tatcactgcc ctatcccgaa aaacgtgaca ccgtcacctt taaaataaca
    10681 acaacaacgc aaacacaaca accgaggaat gcccatgagt atgtcatcca taccgtcgtc
    10741 ctcccaatcc gggaagctct atggctgggt cgaaagaatt ggtaacaagg ttctccatcc
    10801 ttttttgctc tttatctatt tgattatcgt actcatggtg acgacggcaa ttttgtcggc
    10861 ctttggcgtc agtgcgaaaa acccgaccga tggaacgccg gtggtggtga aaaacctgct
    10921 cagtgtggaa ggattacact ggtctttacc caatgttatt aaaaacttta gcggttttgc
    10981 tccacttggt gcgatcctgg cgctggtttt aggtgccggt ctggcggagc gcgtcggctt
    11041 actgccagcg ctaatggtta aaatggcatc gcatgttaat gcccgctacg ccagttatat
    11101 ggtgctgttt attgcttttt tcagccacat ttcttccgat gcgacgttag tgatcatgcc
    11161 accgatgggt gcgctgattt ttctggcggt gggcaggcat ccagttgcag gtttactggc
    11221 tgccattgca ggcgtaggtt gcggctttac ggctaattta ctgattgtca caaccgacgt
    11281 gttgctgtcg gggatcagca cggaagcggc ggctgcgttc aatccgcaaa tgcacgtcag
    11341 tgtaattgat aactggtatt ttatggccag ctccgtagtc gtactgacga ttgttggcgg
    11401 cctgataacc gacaaaatca tcgagccacg gttaggtcaa tggcagggaa acagcgatga
    11461 gaaactgcag acattgaccg aaagtcagcg ttttggttta cgcatagcag gtgtcgtatc
    11521 gctacttttt attgctgcga ttgcgctgat ggtgatcccg gaaaacggga tattgcgcga
    11581 tccgattaat cacaccgtga tgccatcacc ctttattaaa ggtatcgtgc cactgatcat
    11641 tctttttttc tttgttgtct cgctggctta tggcatcgct acccgcacaa ttcgacgtca
    11701 ggcggattta ccgcagttaa tgattgaacc gatgaaagaa atggcaggat ttatcgtgat
    11761 ggtttttccc ctcgcccagt ttgtcgccat gtttaactgg agcaacat
//
LOCUS       DAKISA010000125        11779 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.125, whole genome shotgun sequence.
ACCESSION   DAKISA010000125 DAKISA010000000
VERSION     DAKISA010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11779
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.125"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..612)
                     /locus_tag="OVW45_003629"
     CDS             complement(<1..612)
                     /locus_tag="OVW45_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCV5527983.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRF"
     gene            complement(735..2381)
                     /gene="fumB"
                     /locus_tag="OVW45_003630"
     CDS             complement(735..2381)
                     /gene="fumB"
                     /locus_tag="OVW45_003630"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCV5527984.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(2459..3799)
                     /gene="dcuB"
                     /locus_tag="OVW45_003631"
     CDS             complement(2459..3799)
                     /gene="dcuB"
                     /locus_tag="OVW45_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCV5527985.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(4370..5089)
                     /gene="dcuR"
                     /locus_tag="OVW45_003632"
     CDS             complement(4370..5089)
                     /gene="dcuR"
                     /locus_tag="OVW45_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCV5527986.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTDWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(5086..6717)
                     /gene="dcuS"
                     /locus_tag="OVW45_003633"
     CDS             complement(5086..6717)
                     /gene="dcuS"
                     /locus_tag="OVW45_003633"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCV5527987.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLANSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            6898..7128
                     /locus_tag="OVW45_003634"
     CDS             6898..7128
                     /locus_tag="OVW45_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HCV5527988.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            7140..7412
                     /locus_tag="OVW45_003635"
     CDS             7140..7412
                     /locus_tag="OVW45_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCV5527989.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            7639..7935
                     /gene="ghoS"
                     /locus_tag="OVW45_003636"
     CDS             7639..7935
                     /gene="ghoS"
                     /locus_tag="OVW45_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCV5527990.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            7963..8136
                     /gene="ghoT"
                     /locus_tag="OVW45_003637"
     CDS             7963..8136
                     /gene="ghoT"
                     /locus_tag="OVW45_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HCV5527991.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(8255..9772)
                     /gene="lysS"
                     /locus_tag="OVW45_003638"
     CDS             complement(8255..9772)
                     /gene="lysS"
                     /locus_tag="OVW45_003638"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCV5527992.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKNNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIITEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(10009..11466)
                     /gene="dtpC"
                     /locus_tag="OVW45_003639"
     CDS             complement(10009..11466)
                     /gene="dtpC"
                     /locus_tag="OVW45_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HCV5527993.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMVIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(11525..>11779)
                     /locus_tag="OVW45_003640"
     CDS             complement(11525..>11779)
                     /locus_tag="OVW45_003640"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="constitutive; catalyzes the formation of cadaverine
                     from lysine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCV5527994.1"
                     /translation="TEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEF
                     LQMLCEIGAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
BASE COUNT         3045 a         2857 c         2892 g         2985 t
ORIGIN      
        1 aaagcgccgt agctggcggc aatgaaagcg agcgaggaat atcaccgtcg ttaatgcgcc
       61 aagcagcgcg cagaacatat cggattgcgt gtcccactgg tcgccctggg tgccgagaaa
      121 atcatccgct ccctggccca ttgccagcgc cgcccaccac tcgattaatt catacatcgc
      181 gcttatcgcc agcgccacgc agcagactag gaacgccacc attttacgtc cgcgcacgta
      241 catcccgcgc acgagaattt ctcgtgccac cagtgcaggc accagcccct ggaaaaaatg
      301 ccccagcttg tcatacggat tacggctcaa ccccagccat tcctgcacct caaaaccgat
      361 ggggactttc gcgtaggtgt attgcccgcc gaccatcagg atgatggcgt gaaagaaaat
      421 gagcgtatag agcagcggcg ttaacggata acgtttagcg gtggcaagca gtagcggcac
      481 gacaataatc accggtgtca cttccatcag ccaggtgagt ttgtcatggg ccgaaatgcc
      541 ggtataaatc aggattaacg ttagcgccag tgcgccggtg ttaagaatta acggtttgag
      601 tgtgcgggtc atggtgagtt cgcaagtcag ggaaaatact gactattcac cagcaagcat
      661 gaaattacaa tcgcaaaaga ggtggcccag ggggatcacc tggcagcatg ctgccaggcg
      721 ctgggccgaa gaggttactt agtgcagttc gcgcactgtt tgttgacgat ttgctggaag
      781 aagtcgttac ctttgtcatc gaccaggata aacgccggga aatcttctac ttcgattttc
      841 cagatagctt ccatacccag ctccggataa gcgacgcatt ccagatgctt gatgctctgc
      901 tgcgccagta ccgccgccgg accgccgatg ctaccgaggt agaagccgcc gtgtttatga
      961 cacgcgtcgg taacctgctg gctgcggttg cctttcgcca gcatgatcat gctgccgccg
     1021 tgggattgca gcagatccac ataggagtcc atacgacctg cggtggttgg gccaagtgaa
     1081 cctgatggat aaccggcagg ggttttcgcc ggacccgcgt agtagatcgg gtgatctttg
     1141 atgtactgcg gaagttcttt accggcgtca atcagctctt tcagcttggc gtgtgcaata
     1201 tctcgaccca caataatggt gccagtgagc gacaaacgag tggaaaccgg atattgcgaa
     1261 agctgggcga ggatctcttt catcgggcgg ttaaggtcga ccttcaccgc ttcgccttca
     1321 ccggcctgac gcagttcttg tggaatgtac tgacctggat tgtgttccag tttttcgatc
     1381 cagatacctt cgcggttgat tttcgcttta atgttacggt cagcggagca ggagacgccc
     1441 atgcctaccg ggcaggatgc gccgtgacgt ggcagacgga taacgcgaat gtcgtgcgcg
     1501 aagtatttac cgccaaactg cgcgccaaga ccgagtttct gggcctcttc cagcagttcc
     1561 tgttccagct ggacatcgcg gaacgcctga ccatgttcgt tcccttccgt cggcagttca
     1621 tcgtaatagt gagcgcttgc taatttgacg gttttcaggt tggtttccgc agacgtaccg
     1681 ccaatcacaa acgcgatatg gtacggcggg caggctgcag taccgagggt acgcattttc
     1741 tcgacgagga agtttttcag tttgccggga gtcagcaggg ctttggtttc ctggtagaga
     1801 tacgttttgt tggcagagcc gccgcctttc gcaacgcaaa ggaatttgta ctcatcgcca
     1861 tctaccgcgt acaggtcgat ttgcgcaggc aggttagtgc cggtgttgac ctctttgtac
     1921 atgtccagcg ccgcgttttg cgagtagcgc aggttatctt cgatataggt gttatagacg
     1981 cctttcgaca gcgcttcttc atcaccgcca ccggtccaca cgcgctggcc ttttttaccg
     2041 acgatgatcg cggtgccggt atcctggcag gtcggcagca cacctttggc ggcgatttcg
     2101 gagtttctta agaattgcag tgccacgtac ttgtcgtttt cgctggcttc tggatcgtga
     2161 agaatagccg caacctgttt ctggtgtgcc ggacggagca tgaaagaagc gtcgtgaaag
     2221 gcttgctgcg ccagcagggt cagggcttct ggttccactt tcaggatggt ttcgccgtcg
     2281 aagtcggcaa cgctaacgta atcggaagtg agtagatagt attcggtatt gtctttcccc
     2341 atcgggaaag gtgcctggta gataaagggt ttgtttgaca tagcttccag cctgtaactc
     2401 tgtatttgtt attcgaaaat ggcgtgccgc gtagtcacgg gcggcacgca aagtgcattt
     2461 ataagaaccc gtacatcgcg gcgaagatcc agccgaagac gcacgatacg ctcacaccaa
     2521 tcaaccccgg cagaataaag ctgtggttga tgacgaagcg accgatgtgg gtggtgccgg
     2581 aacggtcaaa ctgaatcgct gccagatcgc tcggataagt cggcaggatg taataaccgt
     2641 agcaagccgg tgctgaagcc acgatgtatg ccggatcaac gccgatcgcc agcgcgaccg
     2701 gaacaatcgc cgccagcgcc gcagcctgag agtttacaaa cttggaaacc agcagcagaa
     2761 caatggcata ggcccacgga tactctttca ccatttcacc cagtacgccc tgaatttcag
     2821 acatatgcgc accgaacatg gtttctgcca tccatgcgat accgtacacc gccacgatgg
     2881 cgatcatacc ggaacggaag acttcgtttt ttgagataga cgcgggattg gttttggtca
     2941 ggataataat cagcgccccg gtcagcagca taaacatctg aataaccagt accatcgaca
     3001 gcggtttgcc gccgaaggat ggacgcaggt ccgaatcagc accaagaagg gcgactacag
     3061 cgattgcccc gaggaaaatc cacattgcca gccagttgct tttcggcagt tttttgtcca
     3121 gcagcgtcgc ggtatcaccg taaacatact cacggttttc cggtacggag atgaatttct
     3181 ggaactcttc gtctttatcc agatctttac cacggaacca gctgaagata ccgatcgcca
     3241 ggataccgat taacgtcgat ggaatggtga ttgccagcag gtcgaggaac tcaagatggc
     3301 gaccatcaaa ggtgacatta cccagcattg caaccagaga cacgaccgca accgacaccg
     3361 gactggcgat gatccccatc tgtgcaccga tagaacttgc cgccatcgga cgttccggac
     3421 ggatgttgtt cttaatggcg acgtcgtaga tgatcggcag aatggtgtaa accacatgac
     3481 ccgtaccgca aagaatggtc agcgtacagg tcacaaacgg cgcgacaatt gagacatatt
     3541 tcgggttgcg gcgcagcagc ttctcggcaa tttgcagcat gacatcaaga ccgcccgaag
     3601 cttgcaaggt cgccgatgcc gccaccaccg caatgataac cagcatgaca tcaaccggtg
     3661 gtttacctgg ctgaaggtgg aagacgaaga ccagaatgac cagaccgata ccgcctaata
     3721 aacccagcgc gataccaccc tttctggcac cataaaacag acatatcagt attatgataa
     3781 gttggatagt aaataacatg tgtgaaccct cgcgataatc ctatttaaat ttttgctgaa
     3841 tagatcacag tcacgttctg ttttgtatga actgttttca gagcctgact aaatatccgt
     3901 ttggtcgtta ccggctttag caaatacctc acagtgaata ttggctgatt aaagcagcgg
     3961 caaatttatg cactgtgata agcggctttt tcagagagaa agactctctg gcgatgggtt
     4021 tatagtttgt actgcgcaga tgttaatgtt ttgtttgctg ttatcttatt gatatttatc
     4081 ggtctatttt ttgttctcag ggcagatgtc attaggttta tagattaatc tgatctaccc
     4141 atttgtgggt aaaaatacac ataatgcggg tgacataata gttaattaac ttttgttagc
     4201 gttttgaaat taaaaacacc gttcacctga agagatatta atttttagcg atgatgcagg
     4261 gataattata tttgatctgg cacaagtttt actgatgaag gatgtaactt gtgccagggg
     4321 tattttgcat tacggtaata attattactt acagataacc agcgatccgt tattggcaat
     4381 attgtttcag tagtgagtag tgttctgcct gaatacggta acggtaaact ggacgccccg
     4441 tgacgccata atggatactg gtgaacaaga tgtggcagtt gaccagccag atgaggtatt
     4501 tacggcagga aacacgcgaa atgttaacct cgttggctag ctcgtcggtt gaaaattcat
     4561 agtcctgatg cgcgtcaatc cactggcaca gtgtgcgtaa cgtctgcggc gttaagcctt
     4621 ttggcaagcg acgaggatcc tgttcgttgg agctgctgcc gtggattagc tgatcaagct
     4681 cggcctggtc ataatactga tgtttttcca gcgccatttt cttttgccgc cagtcggtga
     4741 gcgcctcttc aaagcgggaa gcctggaagg gtttgatcag gtaatccacg acaccgtaat
     4801 gcagcgaatc tttaatggtt gccgcatcgg ctgcggagga gatgacaatc acatcacttt
     4861 tgcaacgcgc gttatgcagg acaggcagta aatcgagccc gttctctttt tgcatataga
     4921 tatcgagcaa tatcaggtcg ataggcgtat cgctattgaa gataatctct ttggctttct
     4981 ccagcgtcga ggctgttcca cagcattgaa agcctgggat ttgtgctacg tatcggcgat
     5041 tcagctccgc gaccattgcg tcgtcatcga taattaatac attgatcatc tgttcgacct
     5101 ctccccgtcc cagggtatct ggacaaaaaa ttgtgtgaaa atcccgggtt ccgattccac
     5161 ggcgatgctg ccgccgagat tttctacctg ttgtttgaca agtgctaaac cgacgcctcg
     5221 ctcgcttcct tttgtcgaga cacctttgtc aaaaatgtga tcgattttgt cgggtgcgat
     5281 ccccggtcca tcatcgttaa cttcgcagtg cagccagccg tgacggtagt gcaatgttac
     5341 gctaatttcg ccaccgggtt ccggccctaa tgcttccagc gcgttttcta tcagatttcc
     5401 caatgtggta atcagcgtcg cgacctggtc ctcactgccg ctgtctggca gctggctttc
     5461 actgtttaaa atcagcgtat ggcctaaatc ggtcgcgcga ttaatcttgc tgattaaaaa
     5521 accagcgata accggagatt tgatcttacc cagcagagag ccaatctctt cctgatagtt
     5581 attggctgtt tttagaatgt aatcttccaa ctgcttataa ctcttcagat gcaataatcc
     5641 gagaatcacg tgcaatttat tcataaattc gtgggatcgt tcacgaagtg cgtcggcata
     5701 gttgaccaga ccgtcgagtc gctgcatcag tttacgtact tcggttttgt ccctgaaggt
     5761 tgaaatggca ccgatgataa cgccattact gcgcaccgga acggtgttga tcagtaatag
     5821 ccggtcttta atcgtaatct cttcgtcgcg gcgcggcgta ccgtcgcgta acacttccga
     5881 gacatctacc acctgtgacc atgagtggct gagcgtcgac agtttctcat cgtcctgcga
     5941 cttacggtaa ttcagcaatt cttgtgcggc atcgttgatc agcgtgacct cgccgcgatc
     6001 gtccacggca acgacgcctt ctttgataga ctgcaacatg gcctggcgtt gctcaaacag
     6061 cgtggagatt tcgtagggtt ccaggccgaa aaggattttt ttcagtacct taaccagaat
     6121 gcaggtgcca atcagtccga ccagcatgcc aaataatacc gaccagataa tgctccagcg
     6181 actgtcattg atttgttggg tcacgcggct taactcaagg ccgatcgcca ccacgccaat
     6241 ttgtttatga ttttcatcgt agatgggggt aaatacgcgt aaagcctgcg ccagaaaacc
     6301 gcgattgata gcgacatttt ctttgccatt cagcgcttta aggatgtcat cacctttaaa
     6361 tggctgacca atacgctggg cttcaggatg cgagtagcga agactttgca tatcggtaac
     6421 gacaataaac agcagatcgt tgcgtttgcg cacggcttcc gcgatggcct ggatgccgct
     6481 ctcctgcggt tttttctgca agccctgacg gatttccggc gagttggcga gggtacgcgc
     6541 cactgccagt gccttgttgg ctagcccgtc tcgcgtcata tcactgattt gcgagaagta
     6601 aatcagatgc accaccaata gcaccgagaa cagtaccgca ctgaccatta agatcactgt
     6661 ggtactcaat ttcatcggac gtttgcgtaa catgcggtag ggcaatgaat gtctcatcag
     6721 cttccttgtg tgacaaattt cttaaacatt atctctgatg aggcgggtaa ttcaaaggga
     6781 gtaagaatga ttggctatat aggggaagag attctggcaa cggaaactgc cagtgctgta
     6841 tgaagattcc agggctatgc ttatagcgat aatcatactg atgagagagg gaaagtcatg
     6901 gatcaggcgc tactggacga gggttatcgc tgttataccg gcgaaaagat cgatgtctat
     6961 ttcaacactg cgatatgtca gcattctggc aattgcgtac gtggcaacgg caagttattt
     7021 aatctcaaac gaaagccgtg gatcatgccg gatgaagtcg acgtcgccac tgtggttaaa
     7081 gtgattgata cgtgcccgag cggcgcgctg aaataccgtc ataaataagc gagggtaaaa
     7141 tggaaatacg cgaaggccac aataaatttt acattaatga cgaacaaggc aagcaaatcg
     7201 ctgaaattgt ctttgtgccg actggagaga atttagcgat tatcgaacat accgatgtcg
     7261 atgaaagcct gaaagggcag gggattggta aacagctggt tgcaaaagtc gtggaaaaaa
     7321 tgcgtcggga aaaacgaaaa attatcccac tatgcccatt tgcgaaacac gaatttgata
     7381 aaacgcggga gtatgatgat attcgcagtt gatgggagag tacagagtca cgatattttt
     7441 catgctctcc gcggagtgat gcaggagagc atctgaaggg tagggggatg cacaaagaat
     7501 gggcagagag cgcgtttttt tgtcccaagt catccccttt actgagcaaa aaaaagaata
     7561 tctcctatat gagaatcatc aatcggggtt atcaagtttt gcgtccccag agcattcaat
     7621 attgatagga gtcatattat ggaaggtaaa aacaagttca atacttatgt tgtttctttt
     7681 gattatccat catcttattc ctcagtgttc ttaaggttaa gatcattgat gtatgatatg
     7741 aatttctctt ctatcgtggc tgatgaatat gggataccac gacaattgaa tgaaaattcc
     7801 ttcgcaataa cgacatcgtt agccgcaagt gaaatcgaag atttaatcag gcttaaatgc
     7861 ttagacttac cggatattga ttttgacctc aacattatga cagttgatga ctatttccgt
     7921 cagttttaca agtagccagg aaggataaga agaacataaa aaatggcact attctctaaa
     7981 atattaattt tttatgtgat tggtgtgaac atatcctttg tcattatctg gtttatctca
     8041 catgagaaaa cacatattcg tttacttagt gcattcctgg tcggaataac ctggccaatg
     8101 agtctgcctg tggcattact tttttctctc ttttaggagc tgtagttgag tggacccgct
     8161 ggttatcgtg aaacgaagcg tctctcctca aaactggatt tgcggaacga cgaacgaagc
     8221 agtcgcctga ctgcttcgtt cattaaagtg aaatttattt ctgtgggcgc atcgccggga
     8281 agagaataac gtcgcgaata gtatgactgt ttgtaaacag cataatcatt cggtcgatac
     8341 caatacccag accagcggtt ggcggcagac catattccag cgcagttacg taatcttcgt
     8401 catagaacat ggcttcgtcg tcacctgcag ctttagcgtt aacctgttcc tggaaacgtt
     8461 cagcctgatc ttctgcgtcg tttaattcgc taaaaccatt accgatttca cgaccaccga
     8521 tgaagaattc aaaacggtcg gtgatttccg ggttaacatc attacggcgc gccagcggtg
     8581 acacttctgc cggatattcc gtaataaagg ttggctgaat cagatgtgct tctgccactt
     8641 catcaaagat ctccgtgata atacgtccca gcccccagct tttctctacc gtaattccga
     8701 tagattcagc taatgcttta gcagcatcaa aattatccag gtcggccata tcggtttctg
     8761 gacgatattt tttgattgct tcgcgcatgg tgagtttttc aaacggtttg ccgaaatcaa
     8821 acacatgctc gccataagtg actttagtgg tacccagaac ctcttgtgcc agggtgcgga
     8881 acagtgactc tgtcagttca atcaaatcgt ggtaatccgc atacgccatg tagagttcca
     8941 tcattgtgaa ctcaggatta tggcgaacag aaataccttc attacggaag ttacggttga
     9001 tttcgaatac ccgttcaaaa ccgcctacaa ccagacgttt cagatacagc tccggcgcga
     9061 tacgcagata catatctaaa tccagagcat tatgatgggt aataaacggg cgagcagatg
     9121 ccccacctgg aattacctgc atcatcgggg tttctacttc cataaagccg cgcgcgacca
     9181 tgaactgacg gatagcggcc agaatttttg aacggacaac aaacgtttga cgggatttat
     9241 cgttagcgat gaggtccaga taacgttgac gataacggac ttcctgatcc tgcagaccat
     9301 ggaatttatc tggtaaagga cgtagtgctt tagtcagcag gcgcaactca gtacagtgaa
     9361 tggaaagctc acccgtttgc gtcttaaaca gcgtaccgcg ggcaccgata atgtcaccca
     9421 gatcccattt tttaaactga tcgttataaa caccttctgg caggctatct cttgcaacgt
     9481 acagttgaat acggccaccg acatcctgca acgttacaaa ggaggctttc cccatgatac
     9541 gacgggtcat cattcggcca gcaaccgaga cttcaatgtt taaggattcc agttcctggt
     9601 tattcttcgc atcaaactct tcgtgcaact ggtcagaggt atggtcgcgg cgaaaatcat
     9661 tgggaaacgc cacaccttgc tgacgtagtg ccgccagttt ttcgcggcga tttctcagtt
     9721 catcgttaaa atcaatagcc tcattggctc cccgtgtttc ttgttcagac attttggttc
     9781 ctctaaatcc agctttcaat ttagcgtaga taaagagaca gatcggtaca ctgtattatc
     9841 tgcctcgact attaataact cagtttatct tatcgaaact aacgcttacc tcaagttgat
     9901 atcactaata aataaccatc aaaatggttt aaatgactta ttaatctcga tttactagtg
     9961 tgattaactg aaagttaatt tattcataac caattgtttt tacgacagtt aatcgttgct
    10021 ctcctgtatc atattcgtcg gcgtgctaaa cagaaaacgg gtagcaaagg ccaatacgac
    10081 gatgatcgcg acacaagcca acgtccactc tcccatctga gaaaagaatc gctggtaagc
    10141 tgcaattacc ataccgctaa tttgcgactc tgtcgtctgc tgtgccacga cgcctgccag
    10201 ccagttggcg accgcgcctg ttgccagcat ataaataccg gttaatacgc cagacatttt
    10261 cagacgcgtg atttgcgcaa tcgccaccgg gtcaataaag agttctgcaa agcccattag
    10321 cgccaacccg gatatcatca cgcccattga cgcttgaccg tcagccgctg catgtcgggc
    10381 atcaaatgcc aacaacataa agccacaagc catcagcagt aagccaaagg caaacttcag
    10441 ccagacgcgc aatgttgagt tgccgcggct ttctggcgac gccagccagg ccagtacaac
    10501 cccagcgagc atcactgcaa ttgcattcac cgactggaat agtgctgtag gtacttcaat
    10561 attgaatgcc tgacgattca caaagcgatc gataaacaag ctgatggtac tgccgccctg
    10621 ttgtgccagt acccagaaca acgtcccgac aaacatcaac aatacaattt gccaaagagc
    10681 acggcggtgt tcggggaatt tgatcatcat gcgggcaatg atttgtgcgg caatgaggca
    10741 aacgatcgcc agcaaatatc cggaccagtc gttctccagc agcagggtaa aaaatactgg
    10801 ggctaaacag agcatcacca ctaaccagct ccatactggt aaggcaaatt tgacgctagt
    10861 gagcgctttt ttatccatac tacgcgtgga ctggaaatga cgatgaccgc ttaagaaaat
    10921 caacaaaccg ataaacatgc cgccacccgc aagggcaaag ccaacatgcc atccatacca
    10981 ctgagcagcc aggccgcagg cgattggggc ggcgatagaa ccgatattgc ccgcagcata
    11041 cagcagcgaa aaaccgccat cacgtcgatg atcgttctcg tcgtagagct cgccaagcaa
    11101 acagctgatg tttgatttga ataaaccgta gccacaaata atgattgcca gcgccagata
    11161 caggctaaag gttgaatttg tatcaatgcc cagcaccaca tggccaaggg tcattaacag
    11221 cgcgccggca atcactgcag tgcggttgcc gagcaggcgg tcggcaagcc agccgccgag
    11281 aataggggta acgtaaacca gagaagcata tgcgctgaac aggctgatgg catggttatc
    11341 atcaaaacca agctgatggg tgagatagag aatgagtaag gcacgcatgc cgtaaaaact
    11401 gaagtactcc cagatttgga tcgccacgat atagtatatc gcgcgcggct gtgagggtgt
    11461 tttcatgtgt tctccttatg agcaaaaaag ggaagtggca agccacttcc cttgtacgag
    11521 ctaattattt tttgctttct tctttcaata ccttaacggt atagcggcca tcagcctgac
    11581 ggtatgcacc gtgaatatcg gtttcaaatc ccggatagtg agcgccgatt tcacacagca
    11641 tctgcaggaa ctccagaacc ggacggcttt cttcggtgat catttcaccc ggcattacca
    11701 gaggaactcc cggcggatac ggaaggatca tattggcgtt aatacgacct accatttcgt
    11761 cgaggtaaac ttcttcggt
//
LOCUS       DAKISA010000126        11719 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.126, whole genome shotgun sequence.
ACCESSION   DAKISA010000126 DAKISA010000000
VERSION     DAKISA010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11719
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.126"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..173
                     /locus_tag="OVW45_003641"
     CDS             <1..173
                     /locus_tag="OVW45_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCV5527995.1"
                     /translation="RNRRLDFCADAIRHAADDEKLAGIGFHWGFSDQSHFSTVFKQRF
                     GMTPGEYRRKFR"
     gene            complement(345..674)
                     /locus_tag="OVW45_003642"
     CDS             complement(345..674)
                     /locus_tag="OVW45_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCV5527996.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(679..864)
                     /locus_tag="OVW45_003643"
     CDS             complement(679..864)
                     /locus_tag="OVW45_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCV5527997.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(861..3500)
                     /locus_tag="OVW45_003644"
     CDS             complement(861..3500)
                     /locus_tag="OVW45_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCV5527998.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGMVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVIAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHEYELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPINATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3708..4697
                     /gene="ldhA"
                     /locus_tag="OVW45_003645"
     CDS             3708..4697
                     /gene="ldhA"
                     /locus_tag="OVW45_003645"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCV5527999.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4808..5230
                     /gene="hslJ"
                     /locus_tag="OVW45_003646"
     CDS             4808..5230
                     /gene="hslJ"
                     /locus_tag="OVW45_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCV5528000.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(5227..5493)
                     /locus_tag="OVW45_003647"
     CDS             complement(5227..5493)
                     /locus_tag="OVW45_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCV5528001.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5767..9291
                     /gene="nifJ"
                     /locus_tag="OVW45_003648"
     CDS             5767..9291
                     /gene="nifJ"
                     /locus_tag="OVW45_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCV5528002.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPCVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARKCGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9657..10790
                     /gene="ompN"
                     /locus_tag="OVW45_003649"
     CDS             9657..10790
                     /gene="ompN"
                     /locus_tag="OVW45_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HCV5528003.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAADGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            10931..11365
                     /gene="uspF"
                     /locus_tag="OVW45_003650"
     CDS             10931..11365
                     /gene="uspF"
                     /locus_tag="OVW45_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCV5528004.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
BASE COUNT         2995 a         2973 c         2790 g         2961 t
ORIGIN      
        1 ttcgtaaccg tcgtctcgat ttttgtgcag atgcgattcg ccatgccgca gatgatgaaa
       61 aactggcagg catcggcttt cattggggat tttctgacca gagtcatttt tcaacggtat
      121 ttaagcaacg ctttgggatg acgccaggcg agtatcgacg taaattccgt taactaattc
      181 gcgttgtgtg tactgcaacg ccaattagtt aaaacagata tttgactatg catttttcag
      241 ggcaaaaggc aatttgacag gagtttttga atattcacct tgtttcagga aaatagatca
      301 atcgctttct cctgtagcgt tatcccgata ttgcggtttt gctattaaaa ttttcgcacc
      361 catttaccgt ttatcccttg tacatattcc cccggtttgg cccgagctac gagcttttgc
      421 ccggcgagtt tcgcgatatc atctaccgac acattatttt gctttgccag ttgttggtaa
      481 ctcgctttac gttcggcatt aatgtcggtt actaatttct ctgtttcagc atccgttttc
      541 agcgcaacca gataaccata aaatgtttca cctacccgcc cctgagttct ggcttcatcc
      601 agagtcaatg ccattacatt gctgcttacc agcccaacaa gaaacgcaca aagaagtaat
      661 gttttcttca tcatcgcctc agaaaagatc gctacgagtt tcaagcagct cttcgacatc
      721 tttgtctgcc ttgatgatga tctcatgctc aattttaacg ttcatattga tagtgatcgg
      781 ttccttgggc gcagcgactt caatacgcgg cgtacagcca accagcataa aagatgacgt
      841 caacgcagcc agtaaaattt tcattgtttt tcctcacact cttttccttg cggacagtca
      901 tttcccggca gacgtgcgtt ctgctccaac catgcctgga gattatcgcc aaagcgtaaa
      961 ctgcgccaca gcgtaaaaat attctcttca tgatggtaat taaggtttac cgtaccgctt
     1021 ttaccatcaa cgcgactggt acctgtaatg ttggcctgca tggttaataa accgagatta
     1081 tctaagttaa tttttgtcga tgaccggcta atttccatat agcgcaacca gttaattgct
     1141 gaacccgcag tcatattgtc tttcaccacc gcatctgcgg tgtctttatc cagccgcaag
     1201 gtcattggcc cgctatttgc cagccaacca tctttcacta tccatttttc gttgtttagc
     1261 cataacggca atgcaccact aaatgccccg gacatggcga attgtttcgg attgacggcg
     1321 ctaaccagtt cgctggatga gagattattc agccgcaaca gcgccgggtc atgttgcggc
     1381 atacgtaatt gtttcatcag tacgttaccg cctaacacat cgacgctaac atcagtcaac
     1441 agcaagggtt cttcttcagt ccacggataa cgcccttgca aatcagccgt aatatttttc
     1501 gctgtcacca gattaatcac ttcggcaatt cgcaacgtaa cggggccgcg agtccccaga
     1561 tgccaggctc catcggcaaa acggaaaggc aggacaaaat cgacaccgtt aacctgatta
     1621 tctggcatcc aggcactacc gcctttcaac acgccgtgtc ctcccgcgcg gaatccttgt
     1681 tcaggcgcag cagaaaatgc aacctgagca tacagttcac catcgcgtaa gttcatcttc
     1741 cagtcgggtg gcaccagcgg ctggaataca gtcagtgatt gttttggcca ccaggcgtta
     1801 ccgcgcagac gaataccgtc ccagcgacca tttacccgaa ccgggccaat ctcaccagca
     1861 tgtaaatcgc ctttaaagag gaaatacgtc ggatcgcgcc catcgacgct aaattttaag
     1921 gttgatggcg gtaacacact gccgccagtg aacagcgttt gcccggcgtc cagtgacagc
     1981 gcgccgctga aggagggatg ctgtgcgtca cgtacccaga cgacgggctt gtcgagaatt
     2041 aatcgcggct tttctaccgt catcgtaccg tattgtaact ggtcgaaacc ggtggagagg
     2101 tcggtcagcg taatcgtgct gtcatgccat tcaccttttc ctgcgacatc ccaggtggca
     2161 tttatcggtg taaaactgcc ttttccccag tagcgccact gccagcggcc cgcgtcaggg
     2221 agaaaatcat tcgccagccc atccatatgc agcacgaaat cgcccagttc atattcatgc
     2281 gcctgcaaaa tggcctgcaa acgtccgtca acaccacgtt gggtgacttt tacacccgct
     2341 aaaggccagc ggatttcatc gatgtccagc gaatcgatga ctcttccctt cgaacgtaat
     2401 aacgcgcctg gctcaaaggc cagcgttggg tcagtcagac ttccacttag ctgcgcgggt
     2461 aagcgggcat ataaaatgag gtccgcctgt ttcgcttcac cggtcagctg cagaggcagc
     2521 tggctgttat ccatgcttaa ttttcctggg ccaaaattta gcaccgcgtt gcctttaccc
     2581 gcttgcccct gggtcagcac actcagtcgt ccgctgatca gagcgttctc aagccctgcc
     2641 tgccagttgt cgactttgac acccagtcgg ccactcaaag gaaaacctgc atacggccaa
     2701 ctccagcgac catcgcttac ggtcaattgt tgacgagtaa tttgccacgg caaatcgagc
     2761 aacggatcgc cgttatcccg tgccagcaca atcaattgcc cgctattttc ctgccagtcc
     2821 agctctgcat ccaccagtga cggttcctgc ggcaagttta acgtcgcagt agcatgccca
     2881 cttacaggaa gtccatccgg cacgagcggc atagtaaatt cccccaccag tttaaccggc
     2941 ggctgatttt caaacgcgat gacatccagt tcgctgactg taagttgttg ccctttcagc
     3001 tggccttgaa atttaacttt ttcgccctga taacgcagtt gctgaatatc agaggttaat
     3061 gcgagagaga gttttccctg ccattcctgc cagggagaaa aaatcagttt atcgatattg
     3121 atccaggtgt taggcagcat ggactgccac tgcgcgaggg tttttggagc agctggcgat
     3181 tgctccgtct gcggcaattt cgccagacaa gcagaatcaa gttctaccat gccgacgttc
     3241 aataaccagc ggctgggatg tgaaaggctg gcgttggtga tatgcgcaag ctgacaatct
     3301 cccaccagat aacggagatc gggaatgatt aaacctttac gcgtaatgcg tgggctttca
     3361 tctaatgcaa tacgtgtccc gagcggtagc cagatgcccg ccagcgtggg aacccacagc
     3421 ccgagcgtca tcagcagcgt caacggcaca agaataatca gtaataacag cgcgagaacg
     3481 gctttatatt tacccagcat gggtagttaa tatcctgatt tagcaaaaaa ttaagcattc
     3541 aatacgggta ttgtggcatg tttaaccgtt cagttgaagg ttgcgcctac actaagcatt
     3601 gttgttgatg aatttttcaa tatcgccata gctttcaatt atatttgaaa ttttgtaaaa
     3661 tatttttagt agcttaaatg tgattcaaca tcactggaga aagtcttatg aaactcgccg
     3721 tttatagcac aaaacagtac gacaagaagt acctgcaaca ggtgaacgag tcctttggct
     3781 ttgagctgga attttttgac tttctgctga cggaaaaaac cgctaaaact gccaatggct
     3841 gcgaagcggt atgtattttc gtaaacgatg acggcagccg cccggtgctg gaagagctga
     3901 aaaagcacgg cgttaaatat atcgccctgc gctgtgccgg tttcaataac gtcgaccttg
     3961 acgcggcaaa agaactgggg ctgaaagtag tccgtgttcc agcctatgat ccagaggccg
     4021 ttgctgaaca cgccatcggt atgatgatga cgctgaaccg ccgtattcac cgcgcgtatc
     4081 agcgtacccg tgacgctaac ttctctctgg aaggtctgac cggctttact atgtatggca
     4141 aaacggcagg cgttatcggt accggtaaaa tcggtgtggc gatgctgcgc attctgaaag
     4201 gttttggtat gcgtctgctg gcgttcgatc cgtatccaag tgcagcggcg ctggaactcg
     4261 gtgtggagta tgtcgatctg ccaaccctgt tctctgaatc agacgttatc tctctgcact
     4321 gcccgctgac accggaaaac taccatctgt tgaacgaagc cgccttcgat cagatgaaaa
     4381 atggcgtgat gatcgtcaat accagtcgcg gtgcattgat tgattctcag gcagcaattg
     4441 aagcgctgaa aaatcagaaa attgggtcgt tgggtatgga cgtctatgag aacgaacgcg
     4501 atctattctt tgaagataaa tccaacgacg tgatccagga tgacgtattc cgtcgcctgt
     4561 ctgcctgcca caacgtgctg tttaccgggc accaggcatt cctgacagca gaagctctga
     4621 ccagtatttc tcagactacg ctgcaaaact taagcaatct ggaaaaaggc gaaacctgcc
     4681 cgaacgaact ggtttaatct tgccgctccc ctgcattcca ggggagctta ttcagataat
     4741 ccccaaagac ctttcatctt ctattcttaa aatagccctg agtcagaaac tgtaattgag
     4801 aaccacaatg aagaaagtag ccgcgtttgt tgcgctaagc ctgctgatgg cgggatgtgt
     4861 aagtaatgac aaaattgctg ttacgccaga acagctacag catcatcgct ttgtgctgga
     4921 aagcgtaaac ggtaagcccg tgaccagcga taaaaatccg ccagaaatca gctttggtga
     4981 aaaaatgatg atttccggca gcatgtgtaa ccgctttagc ggtgaaggca aactgtctaa
     5041 tggtgaactg acagccaaag ggctggcaat gacccgtatg atgtgcgcta acccgcagct
     5101 taatgaactc gataacacca ttagcgaaat gctgaaagaa ggtgcacaag tggatctgac
     5161 cgcgaaccag ttaacgctgg cgaccgcgaa acagacatta acttataagc tggcggattt
     5221 aatgaattaa tagctgccac agctcccggc ggcaagtgac tgttcgctac agcgtttgcc
     5281 gttgggtaat gcacacatcc caatcgccgt accatccagt tgacgggcaa cagaaagcga
     5341 accgccgatc attgcacaat ttgcttctcc actactggac atcgacgctt ttaaacctgg
     5401 cgctacgtgc gccgcagtcg cctgctgaac aggttcacta ctacacgccg acaacaataa
     5461 agcggcacac cctacccaaa acgctgctcg catctcttct tcctctgatc ttcaagccaa
     5521 acgacaccgc cataaataat aggcagcaca gagggcggcg tcgagagctg tcctgcgcgt
     5581 tgccccgcat ttttactttt ttatggctat ttttttgccc tctgtttgat caaaacattc
     5641 attacgctga tgtgggggac acaaaagcga aaatgcagaa gaaagccatt tgctaaaatt
     5701 gaaggattac tactgggcgc gcagcaattt cgtgcgcccc tcatttgcgc aatgtaaggg
     5761 tgtcatatga ttactattga tggtaatggc gcggttgctt cggtggcgtt tcgcaccagt
     5821 gaagttatcg ccatctaccc tattaccccc agttccacga tggcagaaca ggctgatgcc
     5881 tgggccggaa acggcttgaa gaacgtttgg ggagacacac catgcgtggt tgaaatgcag
     5941 tcggaagcgg gtgctatcgc taccgtgcat ggcgctttgc agacgggtgc cctttcaaca
     6001 tcgtttacgt catcgcaggg tttgctgctg atgatcccga cactgtacaa actggcaggc
     6061 gaactcacac cgtttgtcct gcatgtagcg gcacgtaccg ttgccacaca tgcactctct
     6121 atttttggcg atcattccga cgtcatggcg gtgcgccaga cgggttgcgc gatgttgtgt
     6181 gcggcaaacg tccaggaagc gcaagacttt gctctcattt cgcacatcgc gacgctgaaa
     6241 agccgcgtgc catttattca tttctttgat ggtttccgca cgtcccacga aatcaacaaa
     6301 attgtcccgc tggccgatga cacgattctt gatctcatgc cgcaggccga aattgatgct
     6361 catcgcgccc gggcgctcaa cccggaacat ccggtgatcc gtggtacgtc cgccaatcct
     6421 gacacttatt tccagtctcg cgaagccacc aacccctggt acaacgcagt ctatgaccat
     6481 gttgaacagg cgatgaatga tttctctgcc gcgacaggtc gtcagtatca gccgtttgaa
     6541 tattacgggc atccgcaagc tgaacgggtg attatcctga tgggctcagc cattggcacc
     6601 tgtgaagaag tggttgatga attgctaacc cgtggcgaaa aagtcggcgt gctgaaagtt
     6661 cgcctgtacc gccccttctc agccaaacac ttgctacaaa ctctaccggg atccgtacgc
     6721 agcgtggcgg tactggacag aaccaaagaa cccggtgccc aggcagaacc gctctatctg
     6781 gatgtgatga ccgcactggc agaagccttt aataatggca agcgcgaaac tctgccccgt
     6841 gtcattggtg ggcgctatgg tctttcatcc aaagaatttg gcccggactg tgtactggcg
     6901 gtatttgccg agctcaacgc ggctaaaccg aaagcgcgct ttacggttgg tatttacgat
     6961 gatgtaacca atctgtccct gccgttgccg gaaaacaccc agccaaactc ggcgaaactt
     7021 gaagccttgt tttatggcct tggtagtgat ggcagcgttt ccgcgaccaa aaacaatatc
     7081 aagattatcg gtaattccac gccgtggtac gcacagggct attttgttta cgactccaaa
     7141 aaagcgggcg gcctgacggt ttctcacctt cgcgtgagcg aacagccgat tcgttccgct
     7201 tatctcattt cccaggctga ttttgttggc tgccaccagt tgcagtttat cgataaatat
     7261 cagatggctg agcgtttaaa acctggcggc attttcctgc tcaacacgcc gtacagcgca
     7321 gatgaagtgt ggtcgcgctt gccgcaagaa gttcaggccg tgttaaacca gaaaaaagcg
     7381 cgcttctatg tgattaacgc ggcgaaaatc gcccgcaaat gtggcctggc ggcccgtatt
     7441 aataccgtca tgcagatggc ttttttccat ctgacgcaaa ttctgcctgg cgatagcgcc
     7501 ctcgcagaat tgcagggtgc gattgccaaa agttacagta gcaaaggcca ggatctggtg
     7561 gaacgcaact ggcaggctct ggcgctggcg cgtgaatccg tagaagaagt tccgttgcaa
     7621 ccggtaaatc cgcacagcgc caatcgaccg ccagtggttt ccgatgccgc ccctgatttc
     7681 gtgaaaaccg taaccgcagc gatgctcgca gggcttggtg atgccctccc cgtttcggcg
     7741 ctgccgccag acggcacctg gccgatgggc accactcgct gggaaaaacg caatatcgcc
     7801 gaagagatcc ccatctggaa agaggaactc tgtacccaat gtaaccactg cgtcgcagct
     7861 tgcccacact cagctattcg cgcaaaagtg gtaccacccg aagcgatgga aaacgcccct
     7921 gccagcctgc attcgctgga tgtgaaatcg cgtgatatgc gcgggcagaa atatgtcttg
     7981 caggtggctc cggaagattg caccggttgt aacctgtgcg tcgaagtttg cccggcgaaa
     8041 gaccgtcaga atccagagat taaagccatc aatatgatgt cgcgcctgga acatgtcgaa
     8101 gaagagaaaa tcaattacga tttcttcctc aacctgccag aaatcgaccg tagcaaactg
     8161 gaacgtattg atattcgtac atcgcagctg attacaccgc tgtttgaata ttccggtgct
     8221 tgctccggtt gtggcgagac gccgtatatt aaattgctga ctcagctcta tggcgaccgg
     8281 atgttgatcg ctaacgccac cggctgttct tcaatttatg gcggtaacct gccctctaca
     8341 ccgtatacca ccgatgccaa cggtcgtggg ccggcatggg cgaactctct atttgaagat
     8401 aatgccgaat ttggccttgg tttccgcctg acggtcgatc aacaccgtgt ccgcgtgctg
     8461 cgtctgctgg atcaatttgc cgataaaatc ccggcggaat tactgacggc gttgaaatca
     8521 gacgctacgc cagaggttcg tcgagaacag gtcgcagctt tacgccagca actcaacgat
     8581 gttgccgaag cacatgaact gctacgtgat gcagatgcac tggtggaaaa atcaatctgg
     8641 ctgattggtg gtgatggctg ggcttacgat atcggctttg gcggtctgga tcatgtattg
     8701 agtttgacgg aaaacgtcaa cattctggtg ctggatacgc aatgctattc caacaccggt
     8761 ggtcaggcgt cgaaagcgac accgctgggt gcagtaacta aatttggcga gcacggcaaa
     8821 cgtaaagcgc gtaaagatct tggcgtcagt atgatgatgt acggtcatgt ttatgtggcg
     8881 cagatttctc tcggcgcgca gctgaaccag acggtgaaag cgattcagga agcggaagcg
     8941 tatccgggac catcgctgat cattgcttat agcccgtgtg aagagcatgg ttacgatctg
     9001 gcactcagcc atgaccagat gcgccaactc acagctaccg gcttctggcc gctatatcgc
     9061 ttcgatcctc gtcgtgccga tgaaggcaaa ctgccgctgg cgctggattc acgcccgccg
     9121 tcagaagcac tggaagaaac gttacttcac gagcaacgtt tccgtcgtct gaattcgcag
     9181 cagccagaag tggcagaaca gttatggaaa gatgctgcag ctgatttgca aaaacgctat
     9241 gacttcctgg cacaaatggc cggaaaagcg gaaaaaagca acaccgatta aatgctctgg
     9301 ataaggatta cccaattcta aaaaaaagcc cggacgactg ttcgggcttg tctttttata
     9361 tgttggaaaa tcagtggcaa tgcaatggcc caacagaaaa taaaatctgt gacaataaag
     9421 gcatataacc cgcgcagaat aacgtataga aaattaaaag tataattttt attttgtatg
     9481 tattatcttc gatacattca ccagacttat tatcatttca gaagaattat ttgattaagg
     9541 ttttacttaa ggcgtaacaa atgatattta tcggctaact gaacttctcc tttaggatgt
     9601 tttcactccc cggaatgggg ataaaaggca aataaaataa ctaaaggatt tattcaatga
     9661 aaagcaaagt actggcactt ttaattcctg ccctgctcgc cgcaggtgct gcacatgcag
     9721 ccgaagttta taataaagac ggcaacaaat tagatctgta tggcaaagtt gatggcctgc
     9781 attatttttc tgataattca gcgaaagatg gcgatcagag ctatgcgcgt ctgggtttta
     9841 aaggcgaaac ccaaattaac gatcaactca ctggctacgg tcaatgggaa tacaatattc
     9901 aggcaaacaa cactgaatct tcaaaaaacc agtcatggac ccgtctggca tttgccgggc
     9961 tgaaatttgc agattacggt tctttcgatt acggacgtaa ttatggcgta atgtacgaca
    10021 tcgaaggctg gaccgatatg ctgcctgaat ttggcggtga ctcttatacc aatgcagaca
    10081 actttatgac tggtcgtgcc aacggcgtcg cgacttatcg taatactgat ttcttcggtc
    10141 tggtaaatgg tctgaacttc gcggtgcagt atcaaggtaa caacgaaggt gccagtaatg
    10201 gtcaggaagg caccaacaac ggacgtgatg ttcgccatga aaatggtgac ggctggggtc
    10261 tttccacaac atatgattta ggcatgggct ttagcgctgg tgcggcatac acctcatctg
    10321 accgcaccaa tgaccaggtt aaccatactg cggcgggtgg tgataaagca gacgcgtgga
    10381 ctgctgggct aaaatacgat gctaacaata tttacctggc aaccatgtat tcagaaacgc
    10441 gtaatatgac cccgtttggc gacagcgatt atgctgttgc aaacaaaacc cagaactttg
    10501 aagtcactgc acagtaccag tttgattttg gtctgcgtcc ggcagtctct ttcctgatgt
    10561 ctaaaggccg tgacctgcac gctgccgatg gtgcagacaa cccggcaggt gttgatgata
    10621 aagatcttgt taaatacgcc gatgttggcg cgacttacta tttcaataaa aacatgtcca
    10681 cttatgttga ctataaaatc aacctgttgg atgaagatga cagtttctac gctgccaacg
    10741 gcatctctac cgatgatatt gtcgctttag gtctggttta tcagttctaa atcctcctgc
    10801 ccgctgttat ggcgggcttt ttctgcttat tcttcctcct ttgatctaaa ttaaaaatgt
    10861 gaactccgtc attacacaaa aagtgtcatc tggcgttaca ctttatgcgg atactaaaac
    10921 aggaggtttt atgaacagaa cgattcttgt ccctatcgat atttccgatt cagaattaac
    10981 tcaacgcgtg attagccacg ttgaggcaga ggcaaagatt gacgatgcag aggttcattt
    11041 cctgacggta ataccttcac tgccttacta tgcctctctg ggtttagcgt attccgcaga
    11101 attaccggca atggatgacc tgaaagcgga agccaaatcg caactggaag agatcattaa
    11161 aaaatttaaa ctgccaaccg acagagtgca tgtccatgtt gaggaaggct cgcccaaaga
    11221 ccgcattctg gaattggcga agaagatccc cgctcatatg atcatcattg cttcccatcg
    11281 accggatatc accacttatc tgctcggttc caacgccgca gctgtagtgc gtcacgcaga
    11341 gtgctccgtg ctggttgtgc gctgacacta acgcccgcac attgctgcgg gctttttgat
    11401 tcatttcgca aatgtgctga cattttcccc tctaatccgt accatacacg ccacagtttt
    11461 tatatcagat ttcttatgct gggcgttccg gcatgacgca tactcttctg atgccatata
    11521 acgaattgag tcgcttttaa atgtcgcaaa atcaagaaat tagtaagaaa gaacaataca
    11581 acctgaacaa gtaagggcaa aaatcacaac tatctgaccc ataagtattt tatttatttt
    11641 tcaatatgtt aaacattatc agcgacaaca ataagctacg ataacccact ctttttttgc
    11701 cccttttttg ccccttcta
//
LOCUS       DAKISA010000127        11697 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.127, whole genome shotgun sequence.
ACCESSION   DAKISA010000127 DAKISA010000000
VERSION     DAKISA010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11697
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.127"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(350..1603)
                     /locus_tag="OVW45_003651"
     CDS             complement(350..1603)
                     /locus_tag="OVW45_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCV5528005.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            1710..2603
                     /locus_tag="OVW45_003652"
     CDS             1710..2603
                     /locus_tag="OVW45_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCV5528006.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPASRNFRIHLLNALR"
     gene            2738..3958
                     /gene="mlc"
                     /locus_tag="OVW45_003653"
                     /gene_synonym="dgsA"
     CDS             2738..3958
                     /gene="mlc"
                     /locus_tag="OVW45_003653"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCV5528007.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            4083..4778
                     /gene="bioD"
                     /locus_tag="OVW45_003654"
     CDS             4083..4778
                     /gene="bioD"
                     /locus_tag="OVW45_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCV5528008.1"
                     /translation="MLERFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEVIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4731..6023)
                     /gene="clcB"
                     /locus_tag="OVW45_003655"
     CDS             complement(4731..6023)
                     /gene="clcB"
                     /locus_tag="OVW45_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCV5528009.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQASDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(6182..6796)
                     /gene="dmsD"
                     /locus_tag="OVW45_003656"
     CDS             complement(6182..6796)
                     /gene="dmsD"
                     /locus_tag="OVW45_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCV5528010.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6839..7693)
                     /locus_tag="OVW45_003657"
     CDS             complement(6839..7693)
                     /locus_tag="OVW45_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCV5528011.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFMASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(7695..8312)
                     /gene="dmsB"
                     /locus_tag="OVW45_003658"
     CDS             complement(7695..8312)
                     /gene="dmsB"
                     /locus_tag="OVW45_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCV5528012.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTSDTTGYLANPEEV"
     gene            complement(8323..10746)
                     /locus_tag="OVW45_003659"
     CDS             complement(8323..10746)
                     /locus_tag="OVW45_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HCV5528013.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVEYDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEVIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            complement(10807..>11697)
                     /locus_tag="OVW45_003660"
     CDS             complement(10807..>11697)
                     /locus_tag="OVW45_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCV5528014.1"
                     /translation="ADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPI
                     YWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRK
                     DPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYAS
                     TFEGWDSPERSTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIAT
                     GDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLR
                     PSPLAKGNPQHTNLVEIEKI"
BASE COUNT         2957 a         2967 c         3021 g         2752 t
ORIGIN      
        1 cagacttccg tgagtggttg gtttcagcgt gtaacgatat gtacaaacgc tgaataaatt
       61 acagcattga taataacgta ttgtgactta agggaaattt agctaccaat aatagtagtc
      121 ttgatcgggt taactatttt actacttaca aagcggtaga ataactgcgc atcagtaata
      181 aatgacacac agcaaaatga atccgtttat ttgggtactt ataatcctga tgacgctaga
      241 cgcgctgcgg gaattggctg gcgcttcgtc tattttagga tggctattaa cgttggtttg
      301 agcaagcaat aagtccggac gtgtagttac cgcagtccgg gcttattttt caggcgtgca
      361 gacgacgatg caaacgcgtc ccgaccagca gagcaatgac cagcatcaga gcaataaatg
      421 cgccgacgcc gttccagcca tagttatgcc agaaaacacc acccagcgtc ccggcaatac
      481 tcgaccccag atagtaactg aacagataca gcgaggaggc ctggccttta gcgcgttttg
      541 cgcgggggcc aatccagctg ctggcaactg agtgggctgc gaagaatcct gctgagaaga
      601 gtaacattcc ggcaaagatc agccacagcg agctgaataa ggtcatcagt aaaccaaaca
      661 gcataacccc cgtcgaaaac aacatcaccg gaccgcgccc gtagcgggtg gtcatggttc
      721 cggctttggg tgagctccat gtaccggtca aataagccag cgataataag ccaaccacgg
      781 cctgactgac atgccagggg gaaagcatca accgatagcc gatgtaatta aacagcgtga
      841 cgaacgaccc catcagcaaa aagccttctg cgaacaataa cggtaatccc cggtcacgcc
      901 agtgcagacg aaagttgata aataacgtct tagggcgcag cgaagtcggg cgaaaatggc
      961 gtgattccgg aaggattttc cagaacatca acgccgaggc tagcgcgaaa caaccgattg
     1021 ccgccagagc aattcgccag ttgaaaaagt ccgtgaagac accactaatt aagcgtccgc
     1081 tcatgccgcc aattgagttg ccgctgatat acaaccccat cgaaaaggcc acgaaactgg
     1141 ggtggatttc ctcgctaaga taagtcatgc caacagctgc cacgccactt aacgaaagcc
     1201 caatcaaggc gcgcataatc aaaatgccgt gccagctggt catcattgtc gaaagtaacg
     1261 tacaaatgga ggccaacagt agcgccgtga ccatcactgg tttgcgacca atggcatcgg
     1321 atagggggcc agtaaacagc aaaccaatag ccaacatcgc cgtggaaatg gacagtgaaa
     1381 tactactgtt cgcgggggtt aagccaaact cctgcgaaag caccggaagg ataggctgca
     1441 cacaatagag gagtgcaaag gttgccagtc cggcagagaa cagcgccagg gtgacgcgca
     1501 taaattgcgg cgtaccgcgt ttaataaatt gatttggctg agaaatgctt tgcttgtcag
     1561 tgtcgcttgc cggagcgcca tcaacagttg tagtacggct cacttgaaat ccttgctaaa
     1621 tatgcctgtg gatcaggctt atacataggt taggaaaatc gaattgttct gtctaatata
     1681 ttaataatct caaataagat gttttaaata tgaatattga acttcgtcat ctgcgttact
     1741 ttgttgctgt tgcggaagag ctgcatttcg ggcgcgccgc tgcccgcctg aatatttcgc
     1801 aaccaccgct aagtcagcag attcaggcac tggagcaaca aattggtgcc cgactgctgg
     1861 cacgaactaa tcgcagtgta ttgctgacgg cagcaggaaa acagtttctt gcagatagtc
     1921 ggcaaatcct gtctatggta gatgacgctg ccgctcgcgc tgaaagactg catcagggtg
     1981 aagcggggga gttgcgcatt ggttttactt cgtccgctcc ttttattcgg gcggtgtccg
     2041 atacgttatc gctgtttcgc cgtgattatc ccgatgtcca tttacaaacc cgcgaaatga
     2101 acactcgtga gcaaatcgct ccgctcattg aaggaacgct ggatatggga ttgctgcgta
     2161 acacagcgtt accggagacg cttgaacacg cagtcatcgt ccatgaaccg cttatggcga
     2221 tgatcccgca cgatcatccc ctggcaaata acccgaatgt aacgctggct gaactggcga
     2281 aagaaccctt cgtctttttt gatccgcacg tcgggacagg gctgtatgac gatattctcg
     2341 ggctgatgcg acgttaccat ttgacgcccg tcatcactca ggaggtgggc gaggcaatga
     2401 ccatcatcgg tctggtttcc gccggtctgg gtgtttcaat tttgcctgct tcatttaaac
     2461 gtgttcagct caacgaaatg cgctgggtgc cgattgctga agaggatgcg gtttctgaaa
     2521 tgtggttggt ctggccgaaa catcatgaac aaagtccagc ttcgcgtaac tttcgtattc
     2581 atctgctgaa tgctctcagg tgagggaaat ttcagcgaaa aagcccgaaa aatgtgctgt
     2641 taatcacatg cctaagtaaa aatttgacga cacgtattga agtgcttcac catagcctac
     2701 agattatttc ggagcgcgaa aatataggga gtatgcggtg gttgctgaaa accagcctgg
     2761 gcacattgat caaataaagc agaccaacgc gggcgcggtt tatcgcctga ttgatcagct
     2821 tggtccagtc tcgcgtatcg atctttcccg tctggcgcag ctggctcctg ccagtatcac
     2881 taaaattgtc cgtgagatgc tcgaagcaca cctggtgcaa gagctggaaa tcaaagaagc
     2941 ggggaaccgt ggccgtccgg cggtggggct ggtggttgaa actgaagcct ggcactatct
     3001 ttctctgcgc attagtcgcg gggagatttt ccttgctctg cgcgatctga gcagcaaact
     3061 ggtggtggaa gagtcgcagg aactggcgtt aaaagatgac ttgccattgc tggatcgtat
     3121 catttcccat atcgatcagt tttttatccg ccaccagaaa aaacttgagc gtctaacttc
     3181 gattgccata accctgccgg gaattattga tacggaaaat ggtattgtac atcgcatgcc
     3241 gttctacgag gatgtaaaag agatgccgct cggcgaggcg ctggagcagc ataccggcgt
     3301 accggtttat attcagcatg atatcagcgc atggacgatg gcagaggcct tgtttggtgc
     3361 ctcacgcggg gcgcgcgatg tgattcaggt ggttatcgat cacaacgtgg gggcgggcgt
     3421 cattaccgat ggtcatctgc tacacgcagg cagcagtagt ctcgtggaaa taggccacac
     3481 acaggtcgac ccatatggta aacgctgtta ttgcgggaac cacggctgcc tcgaaaccat
     3541 cgccagtgtg gacagtattc ttgagctggc acagctgcgt ctcaatcaat ccatgagctc
     3601 gatgttacat gggcaaccgt taaccgtgga ctcattgtgt caggcggcat tgcgcggcga
     3661 tctactggca aaagacatca ttaccggggt gggcgcgcat gtcgggcgca ttcttgccat
     3721 catggtgaat ttatttaacc cacaaaaaat actgattggc tcaccgttaa gtaaagcggc
     3781 agatatcctc ttccctgtca tctcggacag catacgtcag caggcccttc ctgcgtatag
     3841 tcagcacatt agcgttgaga gtactcagtt ttctaaccag ggcacgatgg caggcgctgc
     3901 gctggtaaaa gacgcgatgt ataacggttc tttgttgatt cgtctgttgc agggttaaca
     3961 ttttttaact gttctaccaa aatttgcgct atctcaattt gggccaggaa agcataactt
     4021 agactttcaa ggttaattat tttcctagtt tatatttgtg aagcataacg gtggagttag
     4081 tgatgctgga gcgtttcttt attaccggta cagacacttc tgtagggaaa acggtggttt
     4141 cccgcgcatt gctacaagcg ttagcctccc agggaaaaac agttgcggga tataaacccg
     4201 tagcgaaggg gagcaaagag acacccgaag ggctgcgtaa taaagatgcc ctggtgttgc
     4261 agagtgtttc aaccatcgaa ctgccttatg aagcagttaa tcctatcgcg ttaagcgaag
     4321 aagaaagtag cgtggcgcac agttgcccaa tcaattacac cctcatttca aacggcctgg
     4381 caaacctgac cgataaagtc gatcatgtcg tggtagaagg gactggcggc tggcgcagtc
     4441 tgatgaatga tttgcgtcca ctctctgaat gggtggtgca ggaacagttg ccggtgttga
     4501 tggttgtcgg tattcaggaa ggttgcatta accatgcact gctaacagct caggcgattg
     4561 ccaacgacgg gctgccgctc attggctggg tggctaaccg aatcaaccca ggactggcgc
     4621 attatgcgga agtcattgat gtgctgggta aaaaacttcc ggcaccgctc attggtgaac
     4681 tgccttatct gccgcgcgcc gaacagcgag aactggggca atacatccgc ttagctatgc
     4741 tgcgcagtgt gctggcggta gatagagtca cggtgtaacg tccgcgaaat taccgacgca
     4801 atgacgcagg caatcaataa accggggagt agctgatact ccccggtcat ttcacatatc
     4861 atcaacgtcg acataatcgg cgcgtgcgtg gttgccgcca acagtgtcgc catcccggtc
     4921 aatccgagta aaagcgtaat ttcttcgcca tcagggaacc ataatcccag gctacgacca
     4981 tacaacatgc caatggcaag accgataaat agcgtcggtg taaagacacc accgggtgcg
     5041 ccggaaccgc tactcgccag cacggcacac agtttacaaa ggaagatccc ggcaatgatc
     5101 attaacagtg gtggggcggt taaaaaggat tgtacggtgc tatagccgtt gccccacact
     5161 gcaggtgtga acagggaaag cagacccacg atcaacccgc ctagtgccag ttgccagggc
     5221 ggcgcaagtt tgagactcac aaatccacga tgacaggcgt tcattaacgt taacaacagt
     5281 ggtccgcaca gacctgccag cacacctgta ctgataatca gcgcatagtc actagcctga
     5341 accgttactg agagttgtac gctgtagagt aacgcgtcgc tatgattaat aagattgcta
     5401 accagcaacg ccacgacggc ggaaataatc accgggccga gagaggccaa catcatagtg
     5461 ccaaacagca cttcggctat aaataaactg ccagcaagcg gggcgcgata ggccgcagcc
     5521 attcccgccg cggccccaca ggcgatccat aatttccatt cctggcgtgg cgtaaaacgt
     5581 tgggcaaaac aggaggcggc aagggcagct aaaagaatca tcgcaccttc gcgaccaatt
     5641 gcactgccgc tggttactac cagcagcgag gcaagcgatt taaccaggct tgctgcgtaa
     5701 tcgaactgtc catcggtttg caacgcttcc atgtaatcgg tcggcgcatg agggcgttgt
     5761 tgggtaaatt tctgccagcc catcagcaac aaacccgccg ccagtccgcc gagcgccgga
     5821 gttagcagcc gtcgccaggg ggaaaggttt gtcgctgcat tgaccagact gccggagtca
     5881 ttattgagga acaaccactc cagtagcagc atcgcatgac gaaacccggc aacggcaaag
     5941 gccgcgagaa taccgacgac tgttgcgata agcagacggc ggaacatagt gcgcagatcc
     6001 ggatagatgt gtagatgatg catgagcaga aatgggcaat gaaacacggg cgactatttt
     6061 gcggcaaaag aggccaaccg caaaagcagt gcgtgcttta agagataaaa aaacgtgaca
     6121 ttgtaactta ttattgccga atcgcgcgta atcgtcacca tccggcaata ttacggtgat
     6181 cctatcgaaa gagtggttta accgcgacag gaatcaacag ttgtgattgc cattgcgcca
     6241 gcgttaagcg agccagttca cccagtgcac ggtagaaagg gtgttctgct ttttcgataa
     6301 aaacatcaag aaaacgtgtt gaccacggaa aaaggtgcca tgccagcaat tcttcgcatt
     6361 ctgtctggcg accattctcc gccaaccacg cagccatcaa cagcagtgaa ccaaaatgat
     6421 cttccggttc gttttgcttc atttcaaact gaatgccttt ctcgcgcatc cactgacgaa
     6481 gtgccaatgt tgaatcgcca aacagcacag attcgcgatc cagccagacc gatccccacg
     6541 gcggagacgg cagtgcccac gggccgacaa acaaacgctg ccaggcctgg gcgtgagtct
     6601 cttcacactg tgtctgaaaa gccgtcacca gcggcgctaa tgacgcttct ggtagaggcc
     6661 actgagtttc ccagccgtca ctggtgagta ccgcaaccag aggtgcggct tccgcactct
     6721 ctggagcgta ataaaacagc gcgcccaaca cgcgcgccgc gacagaaaaa ttatcttgct
     6781 gtgaaaaatg ggtcattcct aacatcctga aatgtgcggg ttgccccgca ccaatttgtt
     6841 aacctgcaat agccattcct actgtcatgt gcaggccata aaagagcaca cgcccgatca
     6901 tctcaccaac aagtatgagg attagcccca gaacaagtcc ggcaacgtga ggttcacggc
     6961 gacgaatgag cggacaaagc cagcatccaa gacctgcgca taacaatacc acacgccaga
     7021 cctgtaagga ggcgtaatct ggtaccagtg cgctggcttg ttgcacggat gagtgaatag
     7081 acgccagaga caaaccttgc agcacaatca ccccggcaca agcgatcagc gcgagaacgc
     7141 taatgatggc aaatggtgtg gtattaaatg taacgcgggc tgcccgcaga attgcggcag
     7201 caagtattgg accgctcaac aataccgtca ggaagaaagc cagcgtagtg taaccgttat
     7261 gccaggttgg cacagtgtcg atttgataca cgcaggtcat catccagacg aaaatgacgc
     7321 cgagcgccat gctgacaagt aaccagagtt tccccaacgc ttgcggcatt ttaccgatga
     7381 ccgccaccag ccaccatagt ccgccaacgg caaaaaatat ggaaccggca gcaatctcgt
     7441 tactcagacc agaagctccg attcgattca gcgagttaaa cgcccgcagt ggcgatccca
     7501 ggtgcatgac agaggccata aacccgatgc ccatcaacag ccagaggaaa aacatgccac
     7561 ggacaatacg ctgacggtct gcgtcatttt tggcggcgaa ccagccaatt ccgctaacaa
     7621 tcagcgcgcc tactacgcat tgacccaata cggtaaagat aaccagtggc cactcatgcc
     7681 atccatttcc cattttacac ttcctccgga ttagccagat aaccagtggt atcgctggtc
     7741 gggcggctgt tggcgttagg tttgataacg atattgggtt ttgtgaaatg cgcgcgcggc
     7801 agtggcgcga cggcagccag agtgccgtgc ttctggcgca gctcttcaat tggaccgaac
     7861 tcaagtgcac gcagcgggca ggattcgaca catatgggtt gtttcccctc ggcgacgcgc
     7921 gaataacaac catcgcactt cgtcatgtgc cctttttcag cattgtactg tggcgcgccg
     7981 tacggacaag ccatgtggca gtagcggcag ccgatacaaa catcttcatc aaccaccaca
     8041 aagccatctt cacgcttatg cattgcgccg ctggggcaga cttttgtaca tgcggggtca
     8101 tcgcaatggt tacaggagat ggagagataa taagcgaaca cgttctggtg ccagacgcca
     8161 ttatcttcct gccagtcgcc gccagcatat tcataaatac ggcggaaact gacttccggg
     8221 ccaagatctt taaaatcttt gcacgccagt tcgcaggttt tacagccagt acagcggctg
     8281 gaatcgataa aaaatccata ttgtgtggtc acgggctact ccttaaacct tttcgatctg
     8341 gacaagattg ctgtgcgacg ggtttccctt tgccagcggt gaagggcggt gagaggtcag
     8401 aatattgata ctgccgccgt gatcgacccg gtcaccaaac atatccgctt taagccaagc
     8461 accttgcccg atggcggtaa cgccaggcag aatacgcgga gtcacttttg cggcaatcag
     8521 catttctcca ttattgttaa atacccgcac ggtatcgcca tgacggatac cgcgtgcctg
     8581 agcatcaatg gggttgatcc acacctcttg tgggcaggcc tgctgtaaca catcaatatt
     8641 gccgtagctg gagtgggtac gcgctttgta atggaagccc gttaactgca gtggataggt
     8701 tttccgcagg ggatcgtccc agccatcaaa acctggggta tacgcaggaa ggggatgaat
     8761 aacttcatct tttttcaatt cccaggtatc cgcaatcttc gccagtcgtt cagaataaat
     8821 ttcgattttc cccgaaggtg ttttcaacgg gtttgcctgt ggatcttcac ggaatgcgcg
     8881 gaaagcgacg tagtgttctt ccgggcattt tttcttaaag atcccggtcg ttttcatctc
     8941 ctcgtagtcg ggcatctcag ggttacgttc cttcgttttc gcatggagat atttgatcca
     9001 ttcatgctga ctgcgacctt cagtaaaggt ttgataaacg tctggtccta agcgtttggc
     9061 gacttcactc agcatccagt agatgggttt gcgttcaaat tttgctgagg ttgcgggttg
     9121 ggcgaggatc acatagccca tattccctgc agattcatga gagataaggt cttcttgctc
     9181 tgttggcatc aggtcgggca acaggatatc gcaatactta gccgaggccg tcatgaagtg
     9241 gtcaatgcca acaatcatct cgcacttgct gtcatcctga agcacctcat gggtgtgatt
     9301 gatgtcgcca tgttgattga tcaatgtgtt actggcgtag caccataaaa acttgatggg
     9361 gacatccagt ttttcttttc cacgaacacc atcacgggtc gcggtcattt ccgtaccatg
     9421 gtcgatggca tcagtccatg taaagacgga aatctgcgtt ttaacaggat tctcgagcat
     9481 cgggaaccat tctaccccca gatcccagct accttcgcgt acgcctgagt tgccgccgtt
     9541 tatgccgacg ttaccggtga gaacggaaag catggcaata gcgcgggatg tttgttcgcc
     9601 gttggagtgt cgttgtggcc cccaaccctg acaaatataa gcaggttttg ctgaaccgat
     9661 ctctcgtgcc aactggataa ttttttctgc cgggatgctg gtgatttttg ctgcccattc
     9721 cggcgtttta gctatgccgt caggcccttc gcccagaata taggctttat aatgcgcgtt
     9781 acgtggtgcg ttggcgggca gcgttttttc atcgtaacca acacaatatt tgtcgagaaa
     9841 tggctgatcg accatgtttt cagtaatcag tacccaggca atcgcacagg ccagtgcgcc
     9901 atcggtgcca gggcgaatgg gcagccattc atcttcacgc ccggcagctg tgtcgttata
     9961 acgtggatcg atgacgatca tgcgtgcgtt tgaacgttcg cgggcttgct cgacgtagta
    10021 agtgacacca ccaccgctca tccgcgtttc tgccgggtta tttccgaaca taacgaccag
    10081 tttcgtattg gcgatatcat ccgggctgtt gccatcattg gcaccgaaca tataactcat
    10141 tgcggcactg atctgtgcgg tactgtagct gccatagcga ctgagaaaac caccgcaaga
    10201 gttcatcaga cggtacggga cgtttgagtt ggtgatgttt ccgccatcta cacctgttcc
    10261 gtacaggaca tgtacagctt cattgccgta atcttttagg atccgccgaa gattatcact
    10321 gatggtatcc agggcttcgt cccaacttat ccgttcaaat ttaccttcac cgcgcttgcc
    10381 gacgcgcttc atgggatatt tcaacctatc aggatgattc atccgtcggc ggatagagcg
    10441 cccgcgtaaa cacgcgcgaa cctgatgatt accgtagacg tcgtcacctg tcgtatcata
    10501 ctccacccag tacacggtgt catctttcac atgcaaacgt aacagacagc ggctcccgca
    10561 gttaacggtg caggaactcc agaccgcttt ctcttctacc ggagcctctg ccgcccgaac
    10621 catttgggaa aatggcagag tgaaagcact gcttgccagc gcaaggctgc caagtgcgga
    10681 ggttttcatc agacttctac ggctgatttc agccttcatg agcgcctctg tggtatggat
    10741 tttcatcatt actcacttat tgcttttcaa acaaaatgtc atgccagaat ttatggttgt
    10801 agtgggttat attttttcga tctcgaccag attagtgtgc tgcgggtttc ccttcgccag
    10861 tggtgaaggg cgcagagtgg ttagcgtatt cacacagccg ccatggtcga ttttatcgcc
    10921 agacatattg gcctcgtgcc aggctccctg gcccatagcg ctaaccccag ggagaatgcg
    10981 tggtgttact ttggctggta gccgaacttc gccacgatgg ttaaacaccc gcaccatatc
    11041 gccggtggca atcccacgtt tctgtgcatc tatagggttg atccacactt cctgacggca
    11101 ggctgccttc aggacatcaa tgttgccgta ggtcgagtga gtacgggatt tgtaatggaa
    11161 accaaacagt tgcagtggga aggtactacg ttcaggggag tcccagcctt caaaggttga
    11221 ggcataaact ggcagtgggc ttatcacttc atctttttcc agttcccagg tacgggcaat
    11281 ttccgccagc ttgctggaat aaatttcaat cttaccggaa ggcgttttaa gtggatttgc
    11341 ctcggggtcg tcacgaaatg ctttgtaggc gacaaaatga ccattgggat ctttacgctt
    11401 atagataccc atttttttca gttcgtcgta agacggtaac gccggatctt tggcaagcat
    11461 tttggcgtac agatgttgta accattgttc ctgcgtgcga ccttcagtga acttttgata
    11521 gacgtcaggt ccaagacgtt tcgcgacttc actcaggatc cagtaaatcg gtttgcgttc
    11581 gaatttttcg ctggtgacag gctggaggaa aatgagatat cccatgttac cggcgtagtc
    11641 gttaggaata atatcttcct gctcaacggt catcaggtct ggcagcagaa tgtcggc
//
LOCUS       DAKISA010000128        11670 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.128, whole genome shotgun sequence.
ACCESSION   DAKISA010000128 DAKISA010000000
VERSION     DAKISA010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11670
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.128"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            1044..2132
                     /locus_tag="OVW45_003661"
     CDS             1044..2132
                     /locus_tag="OVW45_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCV5528015.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPDTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            2143..4374
                     /locus_tag="OVW45_003662"
                     /pseudo
     CDS             2143..4374
                     /locus_tag="OVW45_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4367..5503
                     /locus_tag="OVW45_003663"
     CDS             4367..5503
                     /locus_tag="OVW45_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCV5528016.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDMLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            5500..7371
                     /locus_tag="OVW45_003664"
     CDS             5500..7371
                     /locus_tag="OVW45_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HCV5528017.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     DALIATFSDGVRTQLANGQALKEAQCSCGANGMCRHRVMLVLSYQRLCATTQSTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDMRFYSRSS
                     IRFARCDCIESTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGEAKLDHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRS
                     EQENSPAATRRLFSFQAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQ
                     MLSAPLRQPGIVALREYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQ
                     VISGEGEDNLLTLSLPASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPR
                     VMMTKTRAWALDAETAPVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQS
                     AISQAELLANDLSAVGFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            7496..7957
                     /locus_tag="OVW45_003665"
     CDS             7496..7957
                     /locus_tag="OVW45_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HCV5528018.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(7998..8468)
                     /locus_tag="OVW45_003666"
     CDS             complement(7998..8468)
                     /locus_tag="OVW45_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCV5528019.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(8515..9234)
                     /gene="yehT"
                     /locus_tag="OVW45_003667"
     CDS             complement(8515..9234)
                     /gene="yehT"
                     /locus_tag="OVW45_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCV5528020.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(9231..10916)
                     /gene="btsS"
                     /locus_tag="OVW45_003668"
     CDS             complement(9231..10916)
                     /gene="btsS"
                     /locus_tag="OVW45_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCV5528021.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISSP
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            11138..11203
                     /locus_tag="OVW45_003669"
                     /pseudo
     CDS             11138..11203
                     /locus_tag="OVW45_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            11431..>11670
                     /locus_tag="OVW45_003670"
     CDS             11431..>11670
                     /locus_tag="OVW45_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCV5528022.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWF"
BASE COUNT         2885 a         3049 c         2936 g         2800 t
ORIGIN      
        1 catccgctct ccttattaag gagcgcaaca ccagcacctt acccttacac tgcctgtcga
       61 tactggttta cgtatgttgg tacgaaacgt tgctctccgg atcagggtac cgcctgcggc
      121 accacccgtc cagcagccag gacttaaaat tggtccgatc cggcacaacc tcccaacggg
      181 caggattata aatctcattc actgaattgc aatggagtgt ggatatggtt ataatttatt
      241 cgttggtacg aaatgttgca caatcagcaa gttagcgtca ctgtagtaaa taaaaaaacc
      301 tgcctcacca gcaggttttt ttattttctg cgatctactt tccagatatt ttttgccaat
      361 agcccaccat aaatattcac atcccggtca aatggtgcgt tgaatataaa accgatgaat
      421 ttataaataa aaatgagtta tatgataaat taataaaaac agtaatcatt ttattaaagg
      481 gggaaacggt tttccccctt agatgcgttt caatttgccc gttacttcaa caaatcaatc
      541 aaccgtaaca gagccactaa tagctccagt acagcaatga gcacggttaa cttttgtgtc
      601 agcgtgtcca tccgctctcc ttattaagga gcgcaacacc agcaccttac ccttacactg
      661 cctgtcgata ctggtttacg tatgttggac cacgatgttg cactccggat cagggcacca
      721 cctgcagcac cacccgtccg gtagccagga cttgaaatag gttcgatccg gcgcaacgtc
      781 ccaatggcct ggattataaa tctcattatc ttaattgcaa cggggtccag ccgtggttat
      841 aattcgttcg ttggaacacg atgttgcaca ggctgcggtg ttgggcctga aaatagtaaa
      901 taaaagaacc tgcctcacca gcaggttttt ttatttactg tgatctgctt tccagatatt
      961 ttccgctcaa acaactaatg cgccaaacat ttattgcgcg taaaatatcg tttatttcat
     1021 taatacattt cagggatgaa tatatgtcac cgcagaataa tcatctgcag cgtccgcctg
     1081 ctgctgtgtt atacgccgat gaactggcaa aattaaaaca aaatgataac gcaccttgcc
     1141 cgcccggttg gcagttaagt ttgcctgcgg cacgtgcttt tatccttggc gatagcgcgc
     1201 aaaatattag ccgtaaagtg gtgattagtc cctctgctgt cgaacgcatg ttagtcactc
     1261 tcgctaccgg gcgcggtttg atgttggtgg gggaaccgga taccgcaaaa tctctcctgt
     1321 ctgaattact ggcaaccgct attagcggcg atgccgggtt aaccatccag ggcggcgcat
     1381 ctaccactga agatcaaatc aagtacggct ggaactacgc tctgcttatc aatcatggcc
     1441 catcaacgga agcgctggtc cctgccccgc tttaccaggg aatgcgcgac ggcaaaatcg
     1501 tccgtttcga agagataacg cgtacaccgc tggaagtgca agattgtctg ttaggtatgc
     1561 tttctgacag agttatgacg gtgccggaac tcactggtga agccagtcaa ctctatgcgc
     1621 gggaaggctt taatattatt gccactgcca atacccgcga tcgcggcgtc aatgagatga
     1681 gtgccgcgct aaagcgtcgc ttcgattttg aaactgtctt tccgattatg gatttcgctc
     1741 aggagctgga gcttgtcgcc agtgcttcgg cgcgtttgct ggcccatagc ggtattccgc
     1801 ataaagtgcc tgacgcggta ctggagttac tggtccgcac cttccgcgat ctgcgtgcca
     1861 acggcgaaaa gaaaacgtca atggataccc tgacggcaat tatgtccacc gctgaagccg
     1921 tgaacgtggc ccatgctgtg ggcgtccgag cctggttttt agcgaatcgc gcaggtgagc
     1981 cagccgatct ggtggactgc attgccggaa ccatcgtcaa agataacgaa gaagatcgcg
     2041 ctcgtctgcg ccgttacttc gaacaacgag tcgccacaca taaagaagct cactggcagg
     2101 cttattatca agcccgccac cgcctgccgt gaggaaagat gcatgagcga gccgttaatt
     2161 gtcggcatcc ggcatcatag tccggcctgc gcccggctgg tgaaatcgtt aatcgaaagc
     2221 cagcggccac gatacgtatt gattgaaggc ccggctgatt ttaatgaccg ggtagacgaa
     2281 ctgtttttag cccaccagct tccggtagct atttacagtt attgccagta tcaggacggt
     2341 gcagcccccg ggcgtggtgc ctggacgcca tttgctgaat tttcgccgga gtggcaggcg
     2401 ctacaagccg cacgtcgtat tcaggcacaa acttacttca tcgatttgcc ttgctgggcg
     2461 cagagtgaag aagtggacga ttcgcctgat acgcaagagg aaagccaggc cttgctgctg
     2521 cgtgccaccc gcatggataa cagcgacacc ctgtgggatc acttgttcga agatgaaagc
     2581 cagcaaactg cattaccctc tgcactggcg cactattttg cccaactgcg gggcgattcc
     2641 cccggcgatg cactcaatcg tcagcgcgaa gcctttatgg cccgctggat tggatgggcg
     2701 atgcagcaaa acaatggcga cgtgttagtc gtctgcggtg gctggcacgc tcctgcactg
     2761 gcaaaaatgt gacgcgaatg cccgcaggac attaacacgc cggaattgcc ctcgctggca
     2821 gatgccgtta caggttgtta tctcaccccc tacagtgaaa agcgccttga tgtgctggca
     2881 ggataccttt ccggtatgcc tgccccggtc tggcaaagct ggtactggca gtggggctta
     2941 cagcaggcgg gagaacaact gctaaaaacg gttcttaccc gtttgcgcca gcacaaattg
     3001 cctgcttcga ccgcggatat ggctgccgct catctgcatg cgatggcgct ggcacagttg
     3061 cgcggacata aactaccgtt acgcactgac tggctggatg ccatagcagg ctcgctgatt
     3121 aaagaagccc tgaacgcgcc gttgccgtgg agctatcgcg gagttattca tcccgacacc
     3181 gatccgattc tgctaacgtt gatagacaca ttagcgggtg acggattcgg taaacttgcc
     3241 ccttctacgc cacaaccgcc tctgccaaaa gatgtcacct gcgaactgga acgtaccgca
     3301 atctctcttc cggcggagct taccttaaat cgctttaacc ccaatgggct agcgcaaagt
     3361 caggtgttac atcggctggc aatactggag atcccaggga ttgtacgcca gcagggaagc
     3421 acactgacac ttgcaggcaa cggtgaagaa cgctggaaat taacccgccc gcttagccag
     3481 catgcggcat tgattgaggc cgcctgcttt ggtgccacac tccaggaagc cgcacgccat
     3541 aaattagaag ccgatatgct ggacgcgggt ggaatcggca gtatcaccac atgtcttagc
     3601 caggcggcgt tagcgggtct ggcgtccttc agtcaacaat tactggagca actcacatta
     3661 ttaatcgccc aggaaaatca atttgccgaa atgggccagg cgctggaagt gctatatgcc
     3721 ttatggcggc tggatgaaat tagcggtatg caaggcgcgc agatattaca aacgacgtta
     3781 tgcgcggcta tcgatcgcac gctgtggctg tgtgaatcta acggcagacc agatgaaaag
     3841 gagtttcacg ctcacctgca tagctggcag gcgctttgcc atatcctgcg cgatctacat
     3901 agcggcgtta atttacccgg cgtttttctt tctgcggcgg tagccttact ggagcgccgc
     3961 agtcaggcga ttcatgtccc ggcgctggat cgcggcgcgg ctcttggcgc actgatgcgt
     4021 ctggaacatc ccaacgccag tgccgaagcg gcgctgacga tgctggcgct cgcccgtcat
     4081 caactggcct gccaaccagc atttatcgcc ggtttcagta gtcatttaaa tcaactgagt
     4141 gacgacgatt ttattaacgc cctgcccgat ttgcgcgcgg cgatggcctg gctaccgcca
     4201 cgagaacgcg ggacactggc gcatcaggtg cttgagcatt accagcttgc gcagcttcct
     4261 gtttcagcgc tgcaaatgcc gttgcattgt cctccgcaag ccattgcaca tcatcaacaa
     4321 ctcgaacagc aggcactggc atcgctgcaa aactggggag ttttccatgt ctgaactgaa
     4381 cgatcttctg accacccgtg agctacaacg ctggcgatta attcttggcg aagcggcaga
     4441 aacgacgctt tgtgggctgg atgacaacgc ccggcagata gaccacgcgc tggaatggct
     4501 gtatgggcgc gatcctgaac ggctccagcg tggtgaacgc tccggtggat taggtggctc
     4561 aaatctcacc actcctgagt ggatcaacag tattcacacg ctgtttccgc aacaggtgat
     4621 tgagcggctg gaaagcgatg ccgtactgcg ctacggcatt gaagatgtgg tgacgaatct
     4681 cgacatgctg gaacgtatgc agccttctga aagcctgcta cgcgccgttt tgcacaccaa
     4741 acatctgatg aatcccgaag tactggctgc cgcccgccgg atagtgcgcc aggttgttga
     4801 agaaattatg gctcgactgg caaaggaagt tcgtcaggct ttttctggtg tccgcgatcg
     4861 ccgtcgccgc tcatttattc cactggcgcg aaactttgat ttcaaaagta ctctgcgcgc
     4921 taacctgcaa cactggcacc cgcaacacgg caagttgtat atcgaatccc cccgctttaa
     4981 cagccgcatt aaacgccaaa gcgaacaatg gcaactggtc ttactggttg atcaaagcgg
     5041 atcgatggtg gactcggtga tccactctgc ggtgatggcg gcctgtttgt ggcagttacc
     5101 cggcattcgt acccatctgg tggcgtttga cacaagcgtg gttgatctca cggcagacgt
     5161 tgccgatccg gtagagttat taatgaaagt acagttgggc ggcgggacca atatcgccag
     5221 tgccgtggag tatggtcggc aacttattga acaaccagca aaaagcgtca ttatcctcgt
     5281 gagcgatttt tacgaagggg gttcatcatc attgctgacg catcaggtga aaaagtgtgt
     5341 ccagagcggc atcaaagtgc tgggactggc tgcgctcgat agcaccgcaa caccttgcta
     5401 tgaccgcgat acggcccagg cgctggttaa tgtcggcgca caaatagccg ccatgacgcc
     5461 gggcgagctg gcatcatggc ttgcggagaa tcttcagtca tgaattcact acgtccggaa
     5521 ttattagaac tgacaccgca ggccctgacg gcgttaagca atgccggttt tgttaagcgc
     5581 agtcttaagg aactggaaaa tggcaacgtc ccggagatca gccatgagaa cgacgcttta
     5641 atcgccacct tcagtgacgg tgtccgtacc cagctggcga acggccaggc actgaaagag
     5701 gctcagtgca gttgcggggc caacggtatg tgccgtcatc gcgtgatgct ggtgttaagt
     5761 tatcaacgac tttgtgccac cactcagtct acggaaaaag aagaagagtg ggatccggca
     5821 atctggctgg aagaactggc tacccttccc gatgctaccc gcaaacgcgc acaggcgctg
     5881 gtcgctaaag gcatcaccat tgagttgttc tgtgcgccgg gtgaaattcc ctctgcccgc
     5941 ttaccgatga gcgatatgcg tttttattcc cgcagcagta ttcgtttcgc ccgttgtgat
     6001 tgtattgaaa gcacactttg cgaacatgtc gtactggcag tacaggcctt tgtccaggca
     6061 aaagcgcagc aagcggaatt gactcattta atctggcaga tgcgcagcga acacgtcaca
     6121 tcatctgacg atccgtttgc cagcgaagaa ggcaaaacgt gtcgtcaata tgttcaacaa
     6181 ctaagccagg cattatggct gagcggcatc agccagccgc ttattcacta cgaggccgct
     6241 ttcagccgcg cgcagcaagc agcggaacgc tgcagctggc gatgggtgag tgaatcgcta
     6301 cggcaactgc gcgccagcgt tgatgccttc cacgcccgcg ccagccacta tcatgccgga
     6361 gaagcaaagc ttgatcatct gcggctggtg tctttaggta tgcgttgctg gcaggatatt
     6421 gagcattatg gtttacgcat ctggtttacc gatcccgaca ccggcagtat tttgcacctt
     6481 tcgcgcagtt ggccgcgaag tgaacaggaa aactcaccgg cagctacgcg tcggctgttt
     6541 agttttcagg ctggcgcact agcgggcggg caaattgttt cacaagcagc aaaacgcagt
     6601 gccgatggcg agctgctgtt agctacccgc aaccgcttaa gcagcgttgt gccgctgtcg
     6661 cctgatgcct ggcaaatgtt gagcgcgccg ttacgccagc cgggcattgt ggctttgcgg
     6721 gaatatttac gccagcgtcc ccccgcctgc atacggcctc ttaatcaggt cgataactta
     6781 tttattctgc cggtcgctga gtgtatttcg ctcggttggg atagcagccg ccagacgctg
     6841 gatgcgcagg taatcagcgg cgaaggtgaa gataatctcc tgacgttatc ccttccggca
     6901 tctgcctgtt ctccttttgc cgttgaacgc atggcggcgc ttttgcaaca aacagacgac
     6961 cccgtgagtc tggtttctgg ctttgtcagc tttgtagagg ggcaattgac actggaacca
     7021 cgggtgatga tgacaaaaac ccgcgcctgg gcgctggacg cagaaactgc gcctgtggca
     7081 ccgctacctt ctgccagcgt tttgcctgtg ccgtctaccg ctcatcagtt gctgatgcgc
     7141 tgccaggcgt tacttattca actgctccat aacggctggc gctatcagga acagagcgct
     7201 attagtcagg cagagttgct ggcgaatgac ctctccgcgg tcggttttta tcggctggca
     7261 catgtgttgg gacaatttcg taatacagaa agcgaggcac gggtagaagc aatgaataac
     7321 ggtgttttac tttgcgaaca attattcccc atgcttcagc aacaaggatg aaatagtgct
     7381 ttttactaag agttctactc cagttccgga ctgctcacgc cacggtatta ggcatatcct
     7441 atatagcccc tggtgagagt caccagttcc ttgattaaat aaaatggagt tttacatgaa
     7501 ggctttcaat aagctgtttt ccctcgttgt tgcatctgtt ctggttttct ctcttgctgg
     7561 ctgcggtgac aaagaagaat cgaagaaatt cagcgccaat ctgaacggca ctgaaattgc
     7621 cattacctat gtctacaaag gtgacaaggt gcttaagcaa tcttctgaaa ccaaaattca
     7681 atttgcctcc attggtgcaa ccaccaaaga agatgctgcc aagacacttg agccgttaag
     7741 cgccaaatac aaaaacatcg cgggtgttga agaaaaatta acctataccg atacctacgc
     7801 gcaggaaaac gtgactatcg atatggaaaa agtggatttt aaagccctgc agggtatttc
     7861 aggaatcaac gtttctgctg aagatgccaa aaaaggtatc actatggcgc aaatggaact
     7921 ggtgatgaaa gccgctggtt ttaaagaagt gaaataatct gtcggcggcc atgtttcgca
     7981 tggccgccat acccgtctta gcttttcttc acatgctggc gcgctgccag tccacgcaga
     8041 aaataacgta aaaattgatc gccgcattcg cggaagtttt tatgatccgg tgcgcgcatc
     8101 atcgctgtaa tttccggcat cgaaacgcgg aactgctgtt cggtgaggat cgccagaatg
     8161 tcatcggttt tcagcgaaaa cgcgatgcgt aattttttca gcacgatgtt gttattaatg
     8221 cgacgttccg gctccagtgc cggagcagac tcatccttgc cgcgtttttc ataaatcagg
     8281 ccattgagga atgacgacaa aacaatgtcc ggacaacgct gaaaaccctc ttcgtcttct
     8341 ttacgtagcc agacggcgat ctgttccgcg gtggcttcga cattacccag cgccagaata
     8401 cgcaccaggt cattattatt ggctttcaaa atgtagcgca cgctgcgcag aatatcgtta
     8461 cttagcatga ggccttcagg tgttgatgag gcaaaaagcc attttagtag tcttttacag
     8521 gccaatcgcc tcttttaagc ttttcagata acggcggctg accggcacgg ttaagccatt
     8581 acgcaaaatc aactcggcct ggccgttatc ttccagacga atctcctgta aatgcgcgag
     8641 gttaaccaga tactgacgat ggcagcgcag tagtggtgta cgactttcca gggtacgtaa
     8701 tgtcaattcg gtaaagccct ctttcccttc gtggctggta acgtagacac cgctcatccg
     8761 actgctgaca aatgccacat ctttcatttg cagcaaataa atccgactat gccccgtaca
     8821 agggataaat ttcagcgcct gttgattttc cggtaacagc gaaacatcct gcttgctgcg
     8881 ctcctgacgc aatcgcgcca gcgttttctc cagtcgcgct tcatcaattg gcttcagcag
     8941 ataatcaaag gcatgttctt caaaggcttt aatagcgtat tcgtcaaacg cagtgagaaa
     9001 aacaatatac gggcgatgtt ccgggtcaag catccccacc atttccagac cactgatgcg
     9061 cggcatctgg atatcgagaa acagcacatc cgggcgcagt ttatgcaccg cgccgatccc
     9121 ttccacggcg tttgaacact ctccaacgat ttcaatatcg ctctgctcct gcaaaaatac
     9181 acgcaggttc tcccgtgcta acggttcatc atcgacaatt aagactttaa tcatgcctcg
     9241 tccctccatg gtagtcgtaa cgttattcgg gtgtaactat caggctcaca ggcgacgctt
     9301 attccatagt catcgccaaa ccgttcacgt aaacgcttat ccaccagatt catccccagc
     9361 ccactggcat tggttaccgg ttgatacaaa ccggcattgt cttcgatctc cagcatcaaa
     9421 tgttgcccct cacgtcgggc gctgattgcc actcgccctg tatccagcag ttgtgatgtc
     9481 ccatgtttaa tggcgttttc cactatcggt tgcagggtaa acgcgggcaa ttgctgctgg
     9541 gataattctt gcggaatagc aatgttgacc tgcaaccgcg actggaagcg cgccttttca
     9601 atttgcagat aagcgttcac atgttcaatt tcgtcggcga gagtaacaaa ctccgaaggc
     9661 cgctttaagt ttttgcggaa aaaagtggaa agatactgca ccagctggct ggcctgttcg
     9721 ctgtcgcggc ggatcaccgc tttaatggtg ttaagcgcat taaacaaaaa atgaggattc
     9781 acctgggcgt gaagcagttt gatttctgac tgggtgagca tcgctttttg ccgctcatat
     9841 tgcccggcaa gaatctgcgc cgaaagcagt tgcgcaatcc cctcgcccag cgtgcggttt
     9901 attgaactga ataaacggtt tttggcttca tacaatttga tggtgcccat cacgcgctga
     9961 ttttcgccac gcaacggaat taccagcgtc gaccccagtt tgcattgcgg atgcaaagag
    10021 caacgataag gtacttcgtt gccatcagcg tagaccactt caccggtttc aatcgctttt
    10081 aaggtgtaag gcgaagaaat cggtttgccg ggtaaatggt ggtcgtcacc aattccggta
    10141 aaggccagca atttctctcg atcggtaatc gcgactgcac caatatccag ctcctgatac
    10201 agcacctgag ccactttcat gctgttcact tcgttaaacc cctgtcgcaa aatgccttcc
    10261 gtcgaggctg ccactttcag cgcagtggca gaaaaagccg aagtgtattt ttcaaacatc
    10321 gcgcgtttat cgagcaatat acgcataaac agcgccgcgc cgacggtatt ggtgaccatc
    10381 attggcgcag caatattact caccagacgc accgcatctt cataaggtcg ggcgatcgcc
    10441 aggatgatca gcatttgcac catttcagcg acgaacgtga cggcaccggc ggtaatgggg
    10501 ttaaagactt tatcagtgcg cccgcgacgg atcaggatgc tgtgtaccag gccgccgagc
    10561 aatccttcaa cgatggttga gatcatgcaa cttagcgcgg tcatgccccc catcgaatat
    10621 cgatgtaagc cgccggtcag accaaccagc ccaccgacga ccggaccgcc gagtaagccg
    10681 cccattaccg cgcctatcgc acgggtattg gcaatagaat cgtcaatgtg caacccaaac
    10741 caggtgccca tgatgcagaa gatggaaaag acgatgtagc agagaaattt atgcggcaga
    10801 cgaaccgtga cctgcattaa cggtatgaat aatggcgttt tactcattaa ccacgcaatg
    10861 actaaaaaaa cgcacatctg ctgaagcagc agcaacacca gattaaaatc gtacataccc
    10921 gcaaaccaca cttcccttta aaacgcgtaa catacattgc ctgcgtttaa ctttctttga
    10981 actcttgcag aaaaatgaga attcgtgagt acgatcactc aaaatcgcct ggcaaaaata
    11041 aaatcaccct atagatgcac aaaaaacggg caaaactacc tggttcgcaa aactgcgtct
    11101 aaagttaaac cgggacctcg cgagcaaggg tgagacgatg gcgctttaca caattggtga
    11161 agtggcgttg ctttgtgata ttaaccctgt cacgttacgc gcgtgacgca acatttttaa
    11221 aaatcgacga agatcgtcca ggagcgccgt ttttcaaggg ttggatagat gattcgttta
    11281 ccatgtcgac gaacttcgac gaatttcgcc gataaaaagt gtcccataca tgccccattg
    11341 atgccccata aacaatatca ggaattgaca ctggttatcc atacagataa aaataacacc
    11401 tgtacataac cacagttaca ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa
    11461 aaaatcacta aaatgccaaa cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc
    11521 atcagtaaac gttatgacga tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt
    11581 tctgttacac gcaccgcaga taaggattct gcaaaaacat ttgttcagga gactctgaaa
    11641 gatacctggg agtctgctga cgagtggttt
//
LOCUS       DAKISA010000129        11428 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.129, whole genome shotgun sequence.
ACCESSION   DAKISA010000129 DAKISA010000000
VERSION     DAKISA010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11428
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.129"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(42..383)
                     /gene="bamE"
                     /locus_tag="OVW45_003671"
     CDS             complement(42..383)
                     /gene="bamE"
                     /locus_tag="OVW45_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCV5528023.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(532..2193)
                     /gene="recN"
                     /locus_tag="OVW45_003672"
     CDS             complement(532..2193)
                     /gene="recN"
                     /locus_tag="OVW45_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCV5528024.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="OVW45_003673"
     CDS             complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="OVW45_003673"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCV5528025.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            3280..3873
                     /gene="grpE"
                     /locus_tag="OVW45_003674"
     CDS             3280..3873
                     /gene="grpE"
                     /locus_tag="OVW45_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCV5528026.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            complement(3928..5214)
                     /locus_tag="OVW45_003675"
     CDS             complement(3928..5214)
                     /locus_tag="OVW45_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCV5528027.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(5235..6026)
                     /locus_tag="OVW45_003676"
     CDS             complement(5235..6026)
                     /locus_tag="OVW45_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCV5528028.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6193..7554
                     /gene="ffh"
                     /locus_tag="OVW45_003677"
     CDS             6193..7554
                     /gene="ffh"
                     /locus_tag="OVW45_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCV5528029.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            7803..8051
                     /gene="rpsP"
                     /locus_tag="OVW45_003678"
     CDS             7803..8051
                     /gene="rpsP"
                     /locus_tag="OVW45_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCV5528030.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            8070..8618
                     /gene="rimM"
                     /locus_tag="OVW45_003679"
     CDS             8070..8618
                     /gene="rimM"
                     /locus_tag="OVW45_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCV5528031.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            8649..9416
                     /gene="trmD"
                     /locus_tag="OVW45_003680"
     CDS             8649..9416
                     /gene="trmD"
                     /locus_tag="OVW45_003680"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCV5528032.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            9458..9805
                     /gene="rplS"
                     /locus_tag="OVW45_003681"
     CDS             9458..9805
                     /gene="rplS"
                     /locus_tag="OVW45_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCV5528033.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(9882..10364)
                     /locus_tag="OVW45_003682"
     CDS             complement(9882..10364)
                     /locus_tag="OVW45_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCV5528034.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(10380..>11428)
                     /gene="dgcN"
                     /locus_tag="OVW45_003683"
     CDS             complement(10380..>11428)
                     /gene="dgcN"
                     /locus_tag="OVW45_003683"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HCV5528035.1"
                     /translation="TYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNILAS
                     WHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSLAV
                     LTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRF
                     ALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARK
                     TSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMVLY
                     DVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELADHN
                     MYQAKHQRAEKLVR"
BASE COUNT         2803 a         2749 c         3061 g         2815 t
ORIGIN      
        1 attgagcacc ttttttaacg tctttgagag caactttatt attagttacc actcagcgca
       61 ggtttgttat caatattggt caacacaccg ctactgttaa aggtcagcgt cagcgtttgc
      121 tgcgttacac cttcatgacc tggttgctgg cggaagacat agaaccaggt attcgtacca
      181 aatggatcgg acatcagcgg tgtacccaat gcgtacgcaa cttgttgttg cgtcatgcca
      241 acacgtattt tggatacgtc gttagcggtc agatagttcc cctggttgat gtcaggacgg
      301 taaaccactc gctccagagt ggaacagcct gcggtcaaca tcaatagtac tgctgctgca
      361 gcagtcagcg ttttacagcg catagtgatt tgattccttt tcgggcccga gcagtacgtg
      421 gctcatctgt aatatgccga tgataataga cctttcatca ctttaaaacc ttttgctttc
      481 cggtcttacg gcgttttgct gtttactcta accgtgaagc aggaaaaaag tttacgctgc
      541 aagcagttct ttcgcattcg ctagtgtatt acgtgtgact tcactgccac caagcaggcg
      601 cgccagctct tgtaaccgcg cttttttatt cagggattgc atatgcgttt ctgtcatcgc
      661 accatcggtt tctttgctga caaaatagtg ttgatgacca catcccgcga cttgtggcag
      721 gtgggtaaca cacatcacct gagttgattc gccgagttga cgcagcagtt tgccgacaac
      781 tgccgctgtt ggaccgctaa tccctacatc cacttcatca aaaatcagtg ccggggtttc
      841 cattttacgc gccgtgatga cctggattgc cagtgcgatg cgggacaatt caccaccgga
      901 tgcgactttg gcaataggct gcattggctg acctgggttg gtggttaccc gaaactcaat
      961 acgatcggca ccgtcagcgc ccaggtgatg ctcgtcaaat ttaacatcga tcgtaaactg
     1021 cccatgcggc attgagagcg catgcatact gtcggtgatc agctgtgcaa gttcttctgc
     1081 atattgctgg cgttgttggt gtaatgcgcg cgcgatttcc agtgcctgct gatgatgttt
     1141 cgttaccgcc agcgcaagcg tttcttgtga gtcggcctga tcgtccagtt gctgctgttc
     1201 ttccagtagc gactggtaat actgtggcaa tgcctcaggg ctgacgtgat gtttacgtgc
     1261 cagcgaaatc tgttttgaga tgcgctgttc aagttcaaat agtcggttgg gatcgagatc
     1321 cagacgatcg cagtagtggc gcagttcatc gctggcttca gcaatctgga tggtagcttc
     1381 ttccagcata tcaagtacgc cggacagttt gctgtccatg ccaatcaatt cgctcaccag
     1441 ttgtttagcc gtgtaaagct gactttgcag gtttgcgtct tcaccgtcgg ccattaatgc
     1501 caatgcattc tggctggtgg tcagcaattg accgctgttc gccagacgtt tgtactcttc
     1561 gtcgatttgt tcaaactctc cgggctgcgg attaaattcg ttaagttctt ttaattggta
     1621 ttgcagcagt tccgcacggg cggcgcgttc ctgacttaac tgttgatgat gcgcgaggtc
     1681 acggcagctt tgatgccaca actgataacg tgcggtcatt tcctgcagta gagaggtttc
     1741 attggcatag ccatcaagca ggaatttttg gtgctcaggt ttggtgagta attgatgagc
     1801 gtgctgacca tggatctgaa tcagcaactg acccagttcg cgcagttgtg acagaggaac
     1861 agctgtaccg ttgatgaaac cacgggagcg accatcgctg ctgatcacgc gacgaagcaa
     1921 acattcatgc ccgtcttcaa gctggttttc ttccagccag cgcagagccg ctggcgtatc
     1981 tttcagagaa aaacgggcgc acaggtcagc gcgagcagcg ccggtacgca ccatgtcggc
     2041 ttcagcgcga ccaccgagac aaagaccgag ggcatctatt gcaatagatt tacccgcgcc
     2101 ggtctcgcca gttattacgg tcatgccgct atgaaaatca atctcaagct cacgaacgat
     2161 agcaaagttg ctgatggtca gttgtgccaa catagtcgtt ttcctgtatg aaaaaccatt
     2221 actgttattg tgtacagtat aaactggttt tatatacagt aaagaggctg gcgtaaaatt
     2281 agaataattt ttttgaccag ccgagcttgg tgcttaatgt gttgaaataa ctgtaatctt
     2341 tcggatgaat cagattcaga tggtaatcac agcgacgaat caggacatct tcaccttcct
     2401 gaatcggcag tgctatctgg ctgtcgcaac tgatttccag gtcgttacgg cgatgcgaaa
     2461 aacgcagacg gatcgtgctg ctgctgttta tgaccagtgg tcgtgctgac aacgtatgcg
     2521 ggaacatggg caccagggta atcgcatcca gagagggggt cagaatagga ccgcctgcag
     2581 agagggaata ggcggtggag cctgttggcg tcgaaataat tagtccatca gatcgctgag
     2641 aaaacgcaaa gatctcgtcg atatacactt cgaactcaat catatgcgcc actttgcctg
     2701 gatgaagcac cacttcattt atcgcggtgc tgatgcgttt ctggcaatct tgctgacaga
     2761 cttgcgcttc cagcaaaaaa cgtttctcgc tgatgtagtg gccttccagc acatcggcta
     2821 actgttgctg ggcgttatcg gggtcaaggt cagtcaggaa acccaggttg ccacggttga
     2881 ttccaataac tttaatatcg taacgggcga gtgtgcgtgc cgcgcccagc atattaccgt
     2941 cgccaccaac gactaccgcg agatcagcta gttgcccaat ctccgcgagc gtgccagttt
     3001 tcacattctt cagttgcagt tcgtgagcga tttgttgctc aacgatgacc tcgtaacctt
     3061 ttgtgcacag ccagcggtag agcatttcat gtgttgtcag tgcagtgggg tgccgtgggt
     3121 gtcccacaat gccaatacac ttgaaatgat tattcatttt tccgaggtcc ttgttgcgaa
     3181 gattgatgac aatgtgagtg cttcccttga aaccctgaaa ctgatcccca taataagcga
     3241 agttagcgag atgaatgcga aaaaaacgcg gagaaattca tgagtagtaa agaacagaaa
     3301 acgcctgagg ggcaagcccc ggaagaaatt atcatggatc agcacgaaga gattgaggca
     3361 gttgagccag aagcttctgc tgagcaggtg gatccgcgcg atgaaaaaat tgcgaatctc
     3421 gaagctcagc tggctgaagc ccagacccgt gaacgtgacg gcattttgcg tgtaaaagcc
     3481 gaaatggaaa acctgcgtcg acgtactgaa ctggatattg aaaaagccca caaattcgcg
     3541 ctggagaaat tcatcaacga attgctgccg gtgattgata gcctggatcg tgcgctggaa
     3601 gtggctgata aagctaaccc ggatatgtct gcgatggttg aaggcattga gctgacgctg
     3661 aagtcgatgc tggatgttgt gcgtaagttt ggtgttgaag tgatcgccga aactaacgtg
     3721 ccgctggacc cgaatgtgca tcaggctatc gcaatggtgg aatctgatga cgttgcgcca
     3781 ggtaacgtac tgggcattat gcagaagggt tatacgctga atggtcgtac gattcgtgcg
     3841 gcgatggtaa ctgtagcgaa agcaaaagcg taatttctgt tttcgtaata attcacggcc
     3901 ctgcatgcga atgcagggcc gttttcgtta ctccgccaca ctctcgcgca gcggtttcac
     3961 aggaaaaact tttacctgct taatcatatt gtcctgtacg tcgagaatat ctatatcgta
     4021 ctcgccaata cgcacgcggg tgcctgcgac agggatctct tccagtgcct caagaatgac
     4081 gccattaacc gtgcgggcat catcttccgg tagatgccag ttaaaggctt tgttgatttc
     4141 ccgcacgttg gcggtgccat cgataatcac cgaaccgtcg ttttgcggcg tgacctcttc
     4201 ggcaagtgtt ggcgacatcg acgtagtgaa atcgccgaca atctcttcca gaatatcttc
     4261 aaccgtcacc agcccctgaa tgtctccata ctcgttgacg accaggccga ctttcttttt
     4321 gttgcgctga aactttacca actgcgtgct gagcggcgta ccttccggca caaaatagat
     4381 ctcgtccgcg gcgcgcagca tggtttcttt ggtgaactct tttttctccg acatcaaccg
     4441 ccaggcttca cgtactcgca gcatactgat ggcgtcgtcc agcgaatcac ggtagagcac
     4501 gatgcgcccg tgaggtgagt gggagagttg gcgcagaatc gatttccagt catcgttgat
     4561 atcaataccg ataatttcac tgcgcggcac catgatgtca tcaacggtca ttttttccag
     4621 atcgagcacc gacagcagca tatcctgatt gcgacgggaa atttgtgagc gtgattcgtg
     4681 cacgatagtg cgcaactctt ctttgctcaa agagccgcta accacgatat cggttttgat
     4741 acccatcatg cgcatcagca tacgggtgat agcattcagc aaccagacca gcggcatcat
     4801 caaaatttgc agcggagcca gcagaaaact actcggataa gcgacttttt ccgggtacag
     4861 cgcggcaatg gttttcggca atacctcagc aaataccagt acgacaaaag tcagcacacc
     4921 agtcgcaatt gccacgcccg catcgccgta caaacgcatc ccaacaatag tgccgagcgc
     4981 ggaggcaaga atattgacca ggttattgcc gattaacacc aggcttatca ggcggtctgg
     5041 cttacgcagc aatttttcga cgcgtttggc cgagcgatta ccctgtttcg ccatatgtcg
     5101 cagacgatag cggttgaggg tcatcattcc ggtttcggac ccggaaaaat aggctgaaat
     5161 gaccaccatg atgatcagaa taatgatcaa cgtagtagta gaaatgtgtt ccaggggaaa
     5221 ctccttttct gggtttagct gattaactgc tggacaattc ggctgccgaa gtaggccagt
     5281 gtcagaatta ccgcgcccgc aacgttaaac cagacgacgc ggcgtccacg ccatccttca
     5341 tggtaatgtc cccacagcag cacaatatag acaaaccacg ccacgataga gagcacagcc
     5401 ttgtcgatat tttccatgct aaataggttg tgcatgtaga acaggccagt gcaaagcgtg
     5461 agcgttagca gcaccacgcc aatctgcgtg atgtggaaca ttttacgctc gatactcatc
     5521 aatggcggca tttcctggtt aaatgccagc ttcttgttct tcagttggta atcaatccac
     5581 gccagttgca gcgcgtacag ggcggcgata attagcgtgg cataggaaaa gagcgataag
     5641 ccaatgtgca ccagcatccc aggcgttgct tccagatggg tgatgtattc attgggcatg
     5701 aaggtcgcca gcgccaggtt gataagcgca aaggcataga caatgggtag cagcaaccag
     5761 ccacgattgc gagaagccac aatggtcatt accgtacaga tcatcaaact gaccaatgaa
     5821 ccaacgttca gcaggctgag gttttgtccg ctatcaccgt cgggcaggat gcgggcttcc
     5881 agagcgattg cgtggcagac cagcgcaatg accgcagaaa taatagccat gcgccgccag
     5941 ccgccgtttt tttgcagcag accgggaaca atcagcgcaa gactgacgga gtaggcgaca
     6001 agcgcgagca gagcaaaaac gggcatagtg atgtcgacag tttgagcaat aagaaagaga
     6061 agcagtatag cgtcaggtga acgctgctcc aaccgttgca taacaacaaa gacgccttca
     6121 tgttatactg cggcaaaata ctgatgatgt gtcgcgattg cggccaaccg tttccacccc
     6181 aggcgagaga caatgtttga taatttaacc gatcgtttgt cgcgcacgct gcgcaatatc
     6241 agtggccgtg gacgcctcac tgaagacaac gtaaaagata cgctgcgcga agtgcgcatg
     6301 gcgctgctgg aggcggatgt cgctctgccg gtagtgcgtg agtttatcaa tcgcgtaaaa
     6361 gagaaagcgg ttggtcatga agttaataag agcctgacgc cggggcagga gttcgtcaaa
     6421 atcgtccgta acgaactggt tgcggcgatg ggcgaagaga accagaccct gaacctggcg
     6481 gcgcaaccgc ctgcggtcgt gctgatggcg ggcctgcaag gtgccggtaa aacaaccagc
     6541 gttggtaaac tcggtaagtt cctgcgcgag aagcacaaga agaaagtgct ggtggtttct
     6601 gccgacgttt atcgcccggc ggcaatcaaa cagcttgaga cgctggcaga gcaggtgggc
     6661 gttgatttct tcccttctga tgttggtcag aagccggtag atatcgttaa cgcggcgctg
     6721 aaagaagcca aactgaaatt ctacgacgtg ctgctggtgg ataccgctgg tcgtctgcac
     6781 gttgacgaag cgatgatgga cgagatcaaa caagtccatg cgtcgattaa cccggttgaa
     6841 accctgtttg tggttgacgc catgaccggt caggatgcgg ccaatacggc aaaagcattc
     6901 aatgaagcgt taccgcttac cggcgtagtg ttgaccaaag tggacggcga tgcccgcggc
     6961 ggtgcggcgc tctctattcg tcacatcact ggcaaaccga tcaagtttct cggtgttggc
     7021 gagaaaactg aggcgctgga gccgttccat ccggaccgca tcgcgtcgcg tattctcggc
     7081 atgggcgacg tactgtcgct gatcgaagat atcgaaagca aagttgaccg cgcgcaggca
     7141 gagaaattag ccagcaagct gaaaaaaggc gacggcttcg atctcaacga ttttcttgag
     7201 cagctgcgcc agatgaaaaa tatgggcggc atggctagcc tgatgggcaa gctgccgggc
     7261 atggggcaga tcccggataa tgttaagtcg cagatggacg ataaagtgct ggtgcgtatg
     7321 gaagccatca tcaactcgat gacgatgaaa gagcgcgcta agccagaaat catcaaaggt
     7381 tcgcgtaagc gtcgtatcgc tgccggttgc ggtatgcagg tgcaggacgt taaccgcctt
     7441 ctgaagcagt tcgacgacat gcagcgcatg atgaagaaaa tgaagaaggg cggaatggcg
     7501 aagatgatga gaagcatgaa gggtatgatg cccccaggct tccctggtcg ctaatcggcc
     7561 tggcttgttt gccattttgg cactgggatg tgctcgtcat cctcacgtac tgcgtgtacg
     7621 ctccggtgac tgcgcgcact ccggcaccaa actggctgca ccgccgacgg ccttttacag
     7681 cagagttagc aactgttgat tgcaattccc gcaaaaatga gtaaaatttt cgggctttta
     7741 atatgacacc ggactccgtt cctcgatggg gtccggttgt tttattcaca caagaggatg
     7801 ttatggtaac tattcgttta gcacgtcacg gcgctaaaaa gcgtccgttc taccaggttg
     7861 ttgtcgctga cagccgtaat gcacgcaacg gtcgcttcat cgagcgcgtt ggtttcttca
     7921 acccaatcgc tagcgaaaaa gaagaaggca ctcgcctgga tctggatcgc atcgctcact
     7981 gggttggcca gggcgcaact atttctgatc gcgttgctgc gctgatcaaa gaagtaaaca
     8041 aagcagctta atctgtcacg gtggtcatga tgagcaaaca actcaccgcg caagcacctg
     8101 ttgatcccat cgttttggga aaaatgggtt cgtcttacgg tattcgtggg tggctcagag
     8161 tgttttcttc caccgaagac gccgaaagca tttttgacta tcagccctgg tttatccaga
     8221 aggcgggtca gtggcagcaa gtccagctgg aaagctggaa gcaccacaat caggacatga
     8281 tcatcaagct gaaaggcgtt gacgatcgtg atgcggcgaa cctgctgacg aattgtgaaa
     8341 ttgtcgtgga ttcatcgcag ctgcctcagc ttgaagaggg ggactactac tggaaagacc
     8401 tgatgggctg ccaggtagta accactgaag gctacgatct cggtaaagtc gtcgatatga
     8461 tggaaaccgg atctaatgac gttctcgtca ttaaggcaaa cctgaaagat gcgtttggta
     8521 tcaaggaacg tctcgtaccg ttcctcgatg ggcaggttat caagaaagtc gatctcacta
     8581 ctcgttcaat cgaagtagat tgggatcctg gtttttaaac caccggataa acggtaaaag
     8641 acggcgctat gtggattggc ataattagcc tgtttcctga aatgttccgc gcaattaccg
     8701 attacggggt aactggccgg gcagttaaaa atggcctgct gagcatccag agttggagtc
     8761 ctcgcgactt cacgcatgac cggcaccgta ccgtggacga tcgtccttac ggcggcggac
     8821 cggggatgtt aatgatggtg caacccttgc gggacgccat tcatgcagca aaagccgcgg
     8881 cgggtgaagg cgcaaaggtg atttatctgt caccacaggg acgcaagctt gatcaagcgg
     8941 gcgtcagcga actggcaacg aatcaaaaat tgattctggt gtgcggtcgc tacgaaggta
     9001 tagatgagcg cgtgatccaa accgaaattg acgaagaatg gtcaatcggc gattacgttc
     9061 tcagtggtgg tgagttacca gcaatgacgc tgattgactc cgtttcccgg tttattccgg
     9121 gagtactggg acatgaagcc tcggcaacgg aagattcctt tgctgaagga ttgctggatt
     9181 gcccgcacta tacgcggcct gaggtgttag aagggatgga agttccgcca gtgttactgt
     9241 cgggcaacca tgccgagata cgtcgctggc gtttgaaaca gtcgctgggc cgtacctggc
     9301 ttagaagacc tgaacttctg gaaaacctgg ctctgactga agagcaagca aggttgctgg
     9361 cggagttcaa aacggaacac gcacaacagc aacataaaca tgatgggatg gcgtaagccc
     9421 ccgagatatc agtttaccca ggataagaga ttaaattatg agcaacatta ttaagcaact
     9481 tgaacaagag cagatgaagc aggacgtacc ttccttccgt ccgggtgata ccgtggaagt
     9541 gaaagtatgg gttgttgaag gttccaaaaa acgtctgcag gcattcgagg gcgtggttat
     9601 cgctattcgt aaccgcggtc tgcactctgc attcactgtt cgtaaaattt ccaacggcga
     9661 aggcgttgag cgtgtcttcc agactcactc tccggtagtt gacagcattt ctgtcaaacg
     9721 tcgtggtgct gttcgtaaag ctaaactgta ctacctgcgt gagcgtactg gtaaggctgc
     9781 tcgtatcaaa gagcgtctta actaagattt cgcttaagcg acatcctgtt aagaagggct
     9841 ggccaattgg ctggcccttt ttttgtctgt ttgctcgtgt tttaaggggt tgtaatcacc
     9901 actgcgacgc ggcggttctc ggcgcggccc tgggcggtct tgttactggc tatgggatat
     9961 ttttttccta aaccctgtgt ggtgagattg ctgcgtggaa tttgtccacc aatagcccat
    10021 gcatcggcca cgacattcgc ccgtttcaat gataagcctt cgttgtaact gtcttcacca
    10081 tagttatcgg tgtgtccatc catacgggca tgtgttagcc ctgtcgaggc caatttggct
    10141 gccatggttt ggatctgttg ctggctttcc gggagcaatt tgtagtcatt ttttgcgaac
    10201 agaatggcat ctgataagcc cagcgaccag tcgccggcgg attcagtaaa tccataagat
    10261 tgcatagcgg cgacttgctc aggagtaaac tttccctgag gggactggca gccagtcagt
    10321 attagtgagg tgaaaatcag gggtgctacc aggtgcttta tcatatcgat atatccttgt
    10381 tatctcacca gcttttcggc acgctggtgt ttggcctgat acatattgtg atcggcaagc
    10441 tcttgtaatt tttccgcaga ggcgtgctca atggtcatcg cgtaaccaat gcttaatgtc
    10501 atggtggtct gatggccatt atgaagatca aacgggagat taaagatttg tgtcagtgct
    10561 gagcatatct gctgcacttc agattctgac tgtacatcat agagcaccat agcgaattca
    10621 tcgccgccca ggcggtatgc tttatgtcgc agcccgccaa cttcagctaa ccgttttgcg
    10681 atttcaatca agactctatc gcccgtcgca tgaccccagg tatcattgat gtatttgaaa
    10741 ttatcgccat caagaaatag taacgccgac gtttttcggg catcggaatt gttcatcaac
    10801 gtgttgatgc cgctacgaaa cgctgcgcgg ttagccagcc cggttaatgg gtcatgtagc
    10861 gcggtacgta gaagctgcgc atttttagcc tgtaaacgaa gctgccactc ttccatttca
    10921 tccagcagac tgttgaagtc gagagcgaag cggtgaaact cagcgatacg ttcttccgaa
    10981 actcgtcggg aaaaattgcg gttggaacgc acatcatgta cgacatcggt gatatttttc
    11041 agtgcttcca ccaggccatt gtgcaaatgg cgggtgaggg taattgcgat gcctgatgcc
    11101 agcagaatac aaccggtcag tacggcgagc gaaaaccaga taaaatggct gattgaactg
    11161 tcgcgagcgg ttaagcgtac ttcgccaatg gtttcaccat tgtgacgaat cggctgaatg
    11221 atgggggcgg ggaagagcca gtggcttatg aaattgctga aagtatcgcc tggatcctta
    11281 cgggtgtaat gccaggacgc cagaatattt tgctgcttat cacgtacttc tgcagttgaa
    11341 aattgccctt gctggcccag cgctgccagt gtttcagttg ctgcagggcc atcggcaaaa
    11401 acgacagctg cttccagact gtaagtca
//
LOCUS       DAKISA010000130        11349 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.130, whole genome shotgun sequence.
ACCESSION   DAKISA010000130 DAKISA010000000
VERSION     DAKISA010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11349
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.130"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..242
                     /locus_tag="OVW45_003684"
     CDS             <1..242
                     /locus_tag="OVW45_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCV5528036.1"
                     /translation="LGHEFAHAQYYLLDKENFMALLSRTDKKYENKEEARVITIIESR
                     AAKTLGECTRGAHSGLPFYRVDGPLQTMKITGTPE"
     gene            254..703
                     /locus_tag="OVW45_003685"
     CDS             254..703
                     /locus_tag="OVW45_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528037.1"
                     /translation="MMKYLMVLLSLFSGSVLGMGRVNELCGIDSVKTIEIINLPSYVT
                     TLVPLSKEGLNEIYRYKVVVNEIRDLYAGKIIDLLQMKYFRKEKYNNIRWGGSIISKG
                     NNKCEIYFDAFGECGSVNGINVCFEKNEMIGWIKKEIPLLSQKIGGL"
     gene            965..2101
                     /locus_tag="OVW45_003686"
                     /pseudo
     CDS             965..2101
                     /locus_tag="OVW45_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            2271..2549
                     /locus_tag="OVW45_003687"
     CDS             2271..2549
                     /locus_tag="OVW45_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HCV5528038.1"
                     /translation="MGKPDVTDSNLKNIIDDLYRPNAKVGSGSTADAVRYELATGEKV
                     GGRGHVLKAQDYTRALQDWLDKNPTASPGDRAAAENVLQDLQNALGGK"
     gene            2549..2926
                     /locus_tag="OVW45_003688"
     CDS             2549..2926
                     /locus_tag="OVW45_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528039.1"
                     /translation="MNQYIASYIEKMASFSTVLSETVSLANEYWHPETPPVVMLFSQV
                     GKTIVKNFYEMGDIEREKIFKHVEDGMVSLDDELATVVATGLIEAIVTATDSNSLLWQ
                     DLSKFLGDKSKSHALVWKNFGQE"
     gene            3395..4531
                     /locus_tag="OVW45_003689"
     CDS             3395..4531
                     /locus_tag="OVW45_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCV5528040.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSDD
                     KDVIAIDGKTLRHSYDKSRRKGAIHVISAFSTMHSLVLGQIKTDEKSNEITAIPELLN
                     MLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNP
                     EHDSYAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKE
                     PEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFS
                     GIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAESGLS"
     gene            complement(4573..5343)
                     /gene="yafV"
                     /locus_tag="OVW45_003690"
     CDS             complement(4573..5343)
                     /gene="yafV"
                     /locus_tag="OVW45_003690"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCV5528041.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPKMF
                     TSGFAMEAAASSLAQDDVVDWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
     gene            5524..5970
                     /gene="ivy"
                     /locus_tag="OVW45_003691"
     CDS             5524..5970
                     /gene="ivy"
                     /locus_tag="OVW45_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCV5528042.1"
                     /translation="MFKTITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(6013..8457)
                     /gene="fadE"
                     /locus_tag="OVW45_003692"
     CDS             complement(6013..8457)
                     /gene="fadE"
                     /locus_tag="OVW45_003692"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCV5528043.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLDGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            8697..9275
                     /gene="lpcA"
                     /locus_tag="OVW45_003693"
     CDS             8697..9275
                     /gene="lpcA"
                     /locus_tag="OVW45_003693"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCV5528044.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            9481..10248
                     /locus_tag="OVW45_003694"
     CDS             9481..10248
                     /locus_tag="OVW45_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCV5528045.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(10219..10959)
                     /locus_tag="OVW45_003695"
     CDS             complement(10219..10959)
                     /locus_tag="OVW45_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCV5528046.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(11115..>11349)
                     /gene="yafQ"
                     /locus_tag="OVW45_003696"
     CDS             complement(11115..>11349)
                     /gene="yafQ"
                     /locus_tag="OVW45_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HCV5528047.1"
                     /translation="VKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGY
                     RDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
BASE COUNT         3151 a         2539 c         2814 g         2845 t
ORIGIN      
        1 gtttgggaca tgagtttgct catgcgcagt attaccttct tgataaggaa aatttcatgg
       61 ctcttttaag ccgaacagat aagaaatatg agaataaaga agaggcaagg gttattacaa
      121 taattgaatc gcgtgcggcg aaaactttag gtgagtgtac cagaggagct catagtggac
      181 tgccttttta tcgggtggat ggaccgttgc agacgatgaa aataacgggt acgccagaat
      241 agtgaggttt tttatgatga aatatttgat ggttttattg tctctatttt ctggttcggt
      301 attggggatg gggcgagtga atgaattgtg tggaatagat agcgtgaaaa cgatagagat
      361 aattaatctt ccatcgtatg ttaccacgtt agttccgctt agcaaggaag gtcttaatga
      421 aatatacaga tataaagtgg tggtgaatga aattagagat ctttatgctg gtaagataat
      481 tgatttattg caaatgaagt atttcagaaa agaaaaatat aacaatataa gatggggggg
      541 tagtatcatt tcgaaaggta ataataaatg tgaaatttat tttgatgctt ttggtgaatg
      601 tggaagtgtg aatggcatta acgtgtgttt tgaaaaaaat gaaatgatag gttggattaa
      661 aaaagaaatc cctttgctgt cgcaaaaaat aggtgggctt taaatggtag ggcaatattc
      721 aaagacagta taggtaacta tttgtatggc catgtaaggt aaaagaaaag gaataaacca
      781 tgagcggaaa accggcggcg cggcagggcg acatgacccg cagggaaaga tcatcagaag
      841 ttcacttttt gtactaaata attcgcattt tatgtttaaa aattgagata ttccttatta
      901 cctaaagctg ttttttattg cttatacatg atcaaatact tcttacataa ttaaggagaa
      961 caaaatggaa cttaaaaaat tgatggaaca tatttctatt atccccgatt acagacaagc
     1021 ctggaaagtg gaacataaat tatcggatat tctactgttg actatttgtg ccgttatttc
     1081 tggtgcagag ggttgggaag atatagagga ttttggggaa acacatctcg attttttgaa
     1141 gcaatatggt gattttgaaa atggtattcc tgttcacgat accattgcca gagttgtatc
     1201 ctgtatcagt cctgcaaaat ttcacgagtg ctttattaac tgaatgcgtg actgccattc
     1261 ttcagatgat aaagacgtca ttgcaattga tggaaaaacg ctccggcact cttatgacaa
     1321 gagtcgccgc aggggagcga ttcatgtcat tagtgcgttc tcaacaatgc acagtctggt
     1381 catcggacag atcaagacgg atgagaaatc taatgagatt acagctatcc cagaacttct
     1441 taacatgcta gatattaaag gaaaaatcat cacaactgat gcgatgggtt gccagaaaga
     1501 tattgcagag aagatacaaa aacagggagg tgattattta ttcgcggtaa aaggaaacca
     1561 ggggcggcta aataaagcct ttgaggaaaa atttccgctg aaagaattaa ataatccaga
     1621 gcatgacagt tacgcaatga gtgaaaagag tcacggcaga gaagaaatcc gtcttcatat
     1681 tgtttgcgat gtccctgatg aacttattga tttcacgttt gaatggaaag gactgaagaa
     1741 attatgcgtg gcagtctcct ttcggtcaat aatagcagaa caaaagaaag agccagaaat
     1801 gacggtcaga tattatatca gttctgctga tttaaccgca gaaaagttcg ccacagcaat
     1861 ccgaaaccac tggcacgtgg agaataagct gcactggcgt ctggacgtgg taatgaatga
     1921 agacgactgc aaaataagaa gaggaaacgc cgcagaatta ttttcaggga tacggcacat
     1981 cgctattaat attttaacga atgataaggt attcaaggca gggttaagac gtaagatgcg
     2041 aaaagcagcc atggatagaa actatctcgc gtcagtcctt gcggggagcg ggctttcgta
     2101 atctttccct agggagtcag ccgaatgttg ggaaggattt gacggatgaa gagaagaact
     2161 gcttggccgg cggcagtacg ggaagcccgg gaggatgggg accagaagat gagaatagcg
     2221 caagggatcg tatgcagcat gaacggtata aagatgaatt gcgacagaaa atgggaaaac
     2281 ccgacgttac agattcaaat ttgaaaaata ttatagacga tctttatcgg ccaaatgcaa
     2341 aagtaggaag tggaagcaca gcagatgctg ttcgttatga attggccaca ggtgaaaaag
     2401 ttggtggtag aggacatgta ttgaaagcac aggattatac aagggcttta caggattggt
     2461 tggataagaa tccaacagca tctccagggg atcgagcagc agcagaaaac gttcttcagg
     2521 atttacagaa tgctttggga ggcaagtaat gaaccaatat attgctagtt atatagaaaa
     2581 aatggcatca ttttccactg ttttgtctga gacagtctca ttagctaatg aatactggca
     2641 tccagaaacg ccaccggtgg ttatgttgtt ctctcaggtt ggaaaaacga ttgtaaaaaa
     2701 tttttatgaa atgggggata ttgaaagaga aaaaatattt aaacatgtcg aagatggtat
     2761 ggtctcgctt gatgatgaat tggccacagt tgtagctaca gggcttatag aggcaatagt
     2821 cactgcgacc gatagcaatt ctttactttg gcaagattta agtaaatttc tgggtgacaa
     2881 atcaaagtct catgcattag tgtggaaaaa ttttggtcag gagtgatatt attcaagaag
     2941 tattaagctc gctattgaga tcttaatgct tcttgagatt aaattaaagt taggacgaag
     3001 taattgcagg tggaaataaa tccatagtga cgtgtggcaa tggctattag atgttatggt
     3061 gtgatgggct ttggtgattt aagagctaag ttagattgcc ttatgaaaaa gtatggttat
     3121 atatttaatg atgtgtatca tatatttaaa taaaaaacgg ttctctcttg tcaagatagt
     3181 aataggatag actattgtgt tgataaatat aattaagagt ctactttgtt gattttagaa
     3241 gactttggat cagggaaaga tcatcggaag tacacttttt gtactaaata attcgcgcta
     3301 aatgttcaat aattgagata ttccttatta cctaaaacta tttttcatag cttatatatg
     3361 atcaaatact ccttgcataa ataaggagaa caaaatggaa cttaaaaaac tgatggaaca
     3421 tatttctatt atccccgatt acagacaagc ctggaaagta gaacataaat tgtcagatat
     3481 tctactgttg actatttgtg ccgtcatttc tggtgcagag ggttgggaag atatagagga
     3541 ttttggtgaa acacatctcg attttttgaa gcaatatggt gattttgaaa atggtattcc
     3601 tgttcacgat actattgcca gagttgtatc ctgtatcagt cctgcaaaat ttcacgagtg
     3661 ctttattaac tggatgcgtg actgccattc ttcagatgat aaagacgtca ttgcaattga
     3721 tggaaaaacg ctccggcact cttatgacaa gagtcgccgc aagggagcga ttcatgtcat
     3781 tagtgcgttc tcaacaatgc acagtctggt tctcgggcag attaagacgg atgagaaatc
     3841 caatgagatt acagccattc ctgaacttct taacatgctg gatattaaag gaaaaatcat
     3901 cacaactgat gcgatgggat gccagaaaga tattgcagag aagatacaaa aacagggcgg
     3961 tgattattta ttcgcggtaa aaggaaacca ggggcggctt aataaagcct ttgaggaaaa
     4021 atttccgctg aaagaattaa ataatccaga gcatgacagt tacgcaatca gtgaaaagag
     4081 tcacggcaga gaagaaatcc gtcttcatat tgtttgcgat gtccctgatg aacttattga
     4141 tttcacgttt gaatggaaag ggctgaagaa attatgcgtg gcagtctcct ttcggtccat
     4201 aatagcagaa caaaagaaag agccagaaat gacggtcaga tattatatca gttctgctga
     4261 tttaaccgca gagaagttcg ccacagcaat ccgaaaccac tggcacgtgg agaataagct
     4321 gcactggcgt ctggacgtgg taatgaatga agacgactgc aaaataagaa gaggaaatgc
     4381 agcagaatta ttttcaggga tacggcacat cgctattaat attttgacga atgataaggt
     4441 attcaaggca gggttaagac gtaagatgcg aaaagcagct atggacagaa actatctggc
     4501 gtcagtcctt gcggagagcg ggctttcgta gtcttacccc gactctcccc cagccttaaa
     4561 cacaaccccc actcaccgca acctaaactc atccgcatcc tgccatgccg gaaacttttc
     4621 tctatattcc cgcaatgcca tcatcgacag ctccgcatca atgcgcgttg cctgatgcgc
     4681 gtcggcagta gcgataatct cgccttgtgg attaatcacc cggctgtcac cgcgataatg
     4741 gcagccgttg ccatcgctgc cgacgcgatt gcatcccgcc acatacgcct gattctcaat
     4801 cgcgcgggcc gtcagcaatg cctgccagtg cagagagcgc ggagcaggcc agttggcaac
     4861 gtagatggcg aggtcataat cgttgagatt gcgcgaccac accggaaaac gtaagtcgta
     4921 gcacaccaac ggcaaaatac gccagccgcg ccattccaca atcactcgcg cattgcccgc
     4981 tttataatgt agatgctcat ctgccatgcg gaacagatga cgcttatcat aaaaatgtac
     5041 cgtgccgccc ggctcaacca gcaaaaagcg gttaaccgaa ccagactccg tttgtaatgc
     5101 aacactgcct gcaatcagcg cattgcactg ctgcgcctta gctgtcatcc agtccactac
     5161 gtcatcttgt gctagcgacg aagctgccgc ttccatggca aagccgctgg taaacatctt
     5221 cggtagaacg atcacatcgc gcccggtaat accttccagt tgacgatcaa aatgacgcag
     5281 gttggcaggg ccatccatcc acaccagtgg ttgctgcaaa agcgtaatct tcaaaccagg
     5341 cacggtgtac aactccttta tgcgaagggt tttataactt taacacctta tcaggcagtt
     5401 gccttagcgc agaataaatt gataacaaat gctgatattg gaaatatctg atttgcaaat
     5461 tatcgtgtta tcgccaggct ttagaaggtt aataacatgg gcaggataag ctcgggagga
     5521 atgatgttta agacaataac gacagtcgcc gctctggtca tcgccaccag tgcaatggcg
     5581 caggatgatt taaccattag cagccttgca aagggcgaaa ccaccaaagc tgcatttaat
     5641 cagatggtac aagggcataa gctgcctgcc tgggtgatga aaggcggtac ttatactccc
     5701 gcacaaaccg taacgttggg agatgagacg tatcaggtga tgagcgcgtg caaaccgcat
     5761 gactgtggct cgcaacgtat cgctgtgatg tggtccgaga aatctaatca gatgacgggg
     5821 ctgttctcga ctattgatga gaaaacgtcg caagagaaac tcacctggct gaatgtgaac
     5881 gatgcgcttt cgattgatgg taaaacggtg ctgttcgcgg cgttgactgg cagcctggaa
     5941 aaccatccgg atggctttaa ttttaaataa ttagcggata aagaaacgga gcctttcggc
     6001 tccgttattc aattacgcgg cttcaacttt ccgcactttc tccggcaact ttaccggctt
     6061 cgtcgccagc tcttccggat caaagtcatc aacgttaata ctgcgcagac ggctttcttc
     6121 agctttcacc agaatagcgg cttcatcttt atcaatcagc cccttcgcca gcgcgttgtg
     6181 cgccagttca tccagacggg taaacggcag gtttttaccc agctctttac agatccgctg
     6241 atgaattggg tcggcggcaa tcacatccac cagcgcctct tccagcaagc caaccggatt
     6301 atgctcgctc ggcgtcaggt actgaccgcg accaatgcgg gaacgggtgg cgttcggcac
     6361 ttgtaaaatc ttcgccactt tatgatccag cttgtcagaa ggtgccagat aatgacgtcc
     6421 ggtcgggaag atcaccacat tcagcagccc ggcaaccacg cggttcggga agttttgcag
     6481 taaatcatcc atcgcctgtt cagcctgata cagcgcatct tgtacgcccc agtgcaccag
     6541 cggcaggtcg gcttcattac ggccttcgtc gtcataacgc ttcagcacgg cagaggcgag
     6601 gtagagctgg cttaaaatat cccccagacg ggccgagatg cgctcgcgac gtttcaggct
     6661 gccgtccagc actgccatcg agacatcaga aagcagggcg aggttggcgc tcaggcggtt
     6721 caggtgctga tagtagcgtt tagtggcatc gccggttggc gtgctgctgg ttaaaccgcg
     6781 cgtcaggccc agccagaagc tgcgaacttt gttgctaccg acgtgaccga tatgtttgaa
     6841 caacagttta tcgaacgcgt tgacgtcatt gttcttcgcc gcttccatct cttccagcac
     6901 gtacggatgg caacgaatcg ctccttgtcc gaagatcatc atgctgcggg tcagaatgtt
     6961 agccccttca acggtgatgg caatcggtgc gccctggtaa gcacgcgcca ggaagttgct
     7021 ttgcccgagc ataatgcctt tgccaccagt gatatccatc gcatcaataa tcgactgctg
     7081 cccgcggtgg gtacagtgat acttaacgat agccgacagc acggcaggtt tttcgccgag
     7141 cataatgccg taggtaatca gcgatgccgc agcatccatc acgtaggcat taccggcaat
     7201 acgcgccagc ggctcttcaa tcccttccat cttaccgata gagattttga actgacggcg
     7261 aatgtgagca tacgcgcccg ttgccagcgc taccgatttc acgccgccgg ttgagttgga
     7321 aggcagggtg atgccgcggc ctaccgagag gcactccacc agcatccgcc agccttgccc
     7381 ggccattttc ggcccaccga tgatgtaatc gatcggtacg aagacatctt taccgcgcgt
     7441 cggtccgttc tggaacggta cgttcagcgg gaagtggcga cgaccaattt ccacgcccgg
     7501 cgtggtggtc gggatcagcg cacaggtaat acctaaatct tctgcgccac ccagcagctt
     7561 ttctgggtcg gagagtttaa acgccagccc aagcacagtc gcaatcggtg ccagcgtaat
     7621 atagcgtttg ttccaggtca gacgcatccc cagcacctgc tggccctgcc attcgcccat
     7681 gcagacaatc ccggtgtccg gaatcgcgcc cgcatcggaa cccgcttccg ggcttgtcag
     7741 tgcaaagcaa gggatctcct gaccacgcgc cagacgcggc agatagtgat ctttctgctc
     7801 gtcagtgccg taatgttgca gcagctcgcc cgggcctaat gagttcggca cgccgacggt
     7861 aatcgccagg atcccgctca cgccggagag tttttgcagc acgcgagact gggcataagc
     7921 tgagaactcc agtccgccgt actctttttt gataatcatc gcgaagaagc gatgctcttt
     7981 aaggtacgcc cacaactccg gcggcagatc cgccagctca tgagtaatct ggaaatcatt
     8041 cgccatccgg caggcttctt ctaccgggcc gtcgagaaac gcttgctctt cggcggtcag
     8101 gcgcggctgc ggatagttat gcagcttttt ccagtccggc ttgccctgga acaagtcgcc
     8161 ctcccaccag gtggtgcccg catcaatcgc ttctttctca gtgcgcgaca tcggcggcat
     8221 caccttacgg aaaccgcgaa ataccggcgc ggaaatcatc gacttacgca taggcgcaaa
     8281 gttaaatggc acgaggataa tggccagagg caccagtacc cacgccgacc acagaccagc
     8341 aacgccgagg gcggctgtcc aggcgagcaa aatcagactg ctgataaata agctcacgcg
     8401 gtgatagaac aacgcgccga gcaggacaac cgtagcgaga atactcaaaa tcatcataac
     8461 gaaaagcccc ttacttgtag gaggtctgac cacttgtgat gatatggttg tagtggatgt
     8521 aaaaacattt agcaatgtgt ttacaatata attacaacaa agctcacatt gttgctgttt
     8581 ttatccgcac ttcaggtcaa aaagtcctgg tcatagcacc tgcccgtact tctcgctttt
     8641 ggcggtatcc ggtacactgc attttgtcta ttacatttat gctgaaggat atcctcatgt
     8701 accaggatct tattcgtaac gaactgaacg aagcggcgga aacgctggct aactttttaa
     8761 aagatgacgc caatattcac gccattcagc gcgcggcggt cctgttagca gacagcttta
     8821 aagccggtgg caaagtgctt tcctgcggca acggcggttc ccattgcgac gctatgcact
     8881 ttgccgaaga gttgaccggt cgctaccgtg aaaaccgtcc gggctacccg gcgattgcta
     8941 tttctgacgt tagtcatatt tcctgcgtcg gtaatgattt cggtttcaat gatattttct
     9001 cccgctacgt tgaagcggta ggtcgcgaag gcgatgtact gctggggatc tccacctccg
     9061 gtaactctgc aaacgtgatc aaagcgatcg cagcggcgcg tgagaagggg atgaaagtga
     9121 tcaccctgac cggtaaagac ggcggtaaaa tggctggcac ggcggatatc gaaattcgcg
     9181 ttccgcactt tggttatgcc gaccgcattc aggagattca cattaaagtg atccatatcc
     9241 tgatccagtt aattgaaaaa gagatggtta agtaagtctg gcgtaggccg gataaggcgt
     9301 ttacgccgca tccggcactt gtgcgctgat gcctgatgcg acgctgatgc gtcttatcat
     9361 gcctacaaat ctgtacgcga accgtaggcc gaataagacg tttacgccgc atccggcctg
     9421 aaaattctta aatcaatctt cgccgggggc catgcgctcc cgctgttgtg gaggttaccc
     9481 atgtgcgaat tgctcgggat gagcgccaac gtccctaccg atatctgctt tagtttcacc
     9541 ggtctggtgc agcgtggtgg tggaaccggg ccacataaag atggctgggg cattaccttt
     9601 tacgaaggta aaggctgtcg cacatttaaa gatccacaac ccagctttaa ttcccccatc
     9661 gccaaacttg tccaggacta cccgataaaa tcctgttcgg tggtggctca tattcgccag
     9721 gccaatcggg gcgaggtggc gctggaaaat actcacccgt ttacccgtga gttatggggg
     9781 cgtaactgga cttatgccca taacggacaa ctgacgggct acaaatcact ggaaaccggc
     9841 aacttccgcc cggtcggtga aaccgatagc gaaaaagcct tctgctggct cctgcataaa
     9901 ttaacgcagc gttacccgcg cacaccgggc aacatggcgg cagtgtttaa atatatcgcc
     9961 tcactggcgg atgaactgcg gcagaagggc gttttcaaca tgctgctttc tgacgggcgc
    10021 tatgtgatgg cgtattgctc gactaattta cactggatca cccgccgcgc gccgtttggc
    10081 gtggcaacgc tgctggatca ggatgtggaa atcgacttca gctcgcagac cacaccgaat
    10141 gatgtggtca cggtgattgc aacacagccg ctgacgggca atgaaacctg gcaaaagatt
    10201 atgccaggcg aatggcgctt attttgcctc ggggagcgtg tagtttgatg ccagttgtgg
    10261 ctgcacaact tcgtggctta acggcttgct gaccacgtaa cgaccattga ccacagaaac
    10321 ggtcggtggc ttacgggttt gctcaaagta gtcgtaaccc ggcttcagtt gctcccagaa
    10381 gtccttaaag ttggaatatt tatggcgctt catattggcg tcggtcatgc ggaacgggta
    10441 aatactcact tgcacgctcg gctgaccaaa caccagcgca ccagtaacga actggaagat
    10501 ctcatcaata ccctgattgg tcattgcgta gcagccgatg gaaacacaat cgccgtggat
    10561 catcaggtat ttcccttcat aaccatgcgc acggtcatag gcattgggga aaccaatatt
    10621 aatcgctttg tagtaacggc tgtctggttt taactgatta cgctggacgc tataaaaccc
    10681 ttccgggctt ttgaaatcgc cctgacgctg ttttggccct aagccgccgg aatatttaca
    10741 gattttataa ctgtcgagca gctgatattg ctcgcccatt ttgacgtaga gatcgagcgt
    10801 acgttcttcc ttgaagatct ggatgtagac aggggatccc atcaactgct gcttatactc
    10861 tttgctcact ggcgtggtcg agctactgct gcccagcaaa ccggcaaacg aaacgcacgg
    10921 gatcaacaac atcgcaagaa ttaatgcgat tttacgcata ctgcttattc cttgataaaa
    10981 cggttacaca cgccaggacg gcaaaataga tcccaaatcg gaatagtctg gatttggaag
    11041 gctcacatta tcaccaaaag agttttacgc aagcctgtcg gcgcagggtt tacaaatttc
    11101 atcggcagcg tgctttaccc aaagagcgcc gcgtgagttc cagttctctc aaatcgtaaa
    11161 agtttatcgg taagtttgta aatcaggatc cagtccggtt cgacatgagc atcgcgataa
    11221 cctttccatg aaccttgcag cgggtggtct ttataaacag ctggaagcgg taaagcatta
    11281 ttgataagaa gcgtcataag atatttcaat ttattcatat ccttatgacg cttttgtgca
    11341 agttttaca
//
LOCUS       DAKISA010000131        11242 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.131, whole genome shotgun sequence.
ACCESSION   DAKISA010000131 DAKISA010000000
VERSION     DAKISA010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11242
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.131"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..309
                     /locus_tag="OVW45_003697"
     CDS             <1..309
                     /locus_tag="OVW45_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCV5528048.1"
                     /translation="VVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINDLQF
                     FDQQIRWAKADLVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN
                     "
     gene            508..789
                     /gene="ppiC"
                     /locus_tag="OVW45_003698"
     CDS             508..789
                     /gene="ppiC"
                     /locus_tag="OVW45_003698"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCV5528049.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(876..2351)
                     /gene="ilvC"
                     /locus_tag="OVW45_003699"
     CDS             complement(876..2351)
                     /gene="ilvC"
                     /locus_tag="OVW45_003699"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCV5528050.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            2501..3394
                     /gene="ilvY"
                     /locus_tag="OVW45_003700"
     CDS             2501..3394
                     /gene="ilvY"
                     /locus_tag="OVW45_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCV5528051.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(3446..4990)
                     /gene="ilvA"
                     /locus_tag="OVW45_003701"
     CDS             complement(3446..4990)
                     /gene="ilvA"
                     /locus_tag="OVW45_003701"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCV5528052.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(4993..6843)
                     /gene="ilvD"
                     /locus_tag="OVW45_003702"
     CDS             complement(4993..6843)
                     /gene="ilvD"
                     /locus_tag="OVW45_003702"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCV5528053.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSEQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(6908..7837)
                     /gene="ilvE"
                     /locus_tag="OVW45_003703"
     CDS             complement(6908..7837)
                     /gene="ilvE"
                     /locus_tag="OVW45_003703"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCV5528054.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKCIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(7857..8120)
                     /gene="ilvM"
                     /locus_tag="OVW45_003704"
     CDS             complement(7857..8120)
                     /gene="ilvM"
                     /locus_tag="OVW45_003704"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCV5528055.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(8117..9763)
                     /locus_tag="OVW45_003705"
     CDS             complement(8117..9763)
                     /locus_tag="OVW45_003705"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCV5528056.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVACSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGIKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(9766..9816)
                     /locus_tag="OVW45_003706"
     CDS             complement(9766..9816)
                     /locus_tag="OVW45_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528057.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(9903..10001)
                     /gene="ilvL"
                     /locus_tag="OVW45_003707"
     CDS             complement(9903..10001)
                     /gene="ilvL"
                     /locus_tag="OVW45_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCV5528058.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            10354..>11242
                     /locus_tag="OVW45_003708"
     CDS             10354..>11242
                     /locus_tag="OVW45_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HCV5528059.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGRHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNG"
BASE COUNT         2555 a         3090 c         2951 g         2646 t
ORIGIN      
        1 gtcgtgctca acggaaaaat taatggcaac gcatgtatcg tcgttccatt atcaaccact
       61 cgcgatcatg acaagttaaa tcgtggaatg catgttgaaa tagccagtaa tgttatcaat
      121 gacctgcaat tctttgatca gcagattcgt tgggccaaag ccgacctagt acaacaagtc
      181 agtcgaaacc gattaaacag agcaaggact tacagagggt atcttaatca atgtttgcca
      241 catgagttgg ttgcagacat acagagggcg gtcatcaagt caatcaatgc catatcgctc
      301 ataaattgac aataaaataa ccatccccga taatgacttt gtagcccata gaggccctaa
      361 gttttctccc cttgagggac ctgcgaagcc cggattttcc gggcttttgc catatatgta
      421 ccagcccctc cctgactaac aattacgtta gcatctgcgg atcatgctaa aatcgccgcc
      481 tgacaattat tcactcaagg cacgatcatg gcaaaaacag cagcagcact gcatatcctt
      541 gtaaaagaag agaaactggc tctggatctt ctcgagcaga ttaagaacgg tgccgatttc
      601 ggcaagctgg cgaagaaaca ctccatttgc ccatcaggca aacgcggcgg tgatttaggt
      661 gaattccgcc agggtcagat ggttccggcg tttgataaag ttgtgttctc ttgtccggta
      721 ctggagccaa ccggcccgct gcacacccag ttcggttacc acatcattaa ggtgctgtac
      781 cgtaactaat agcaaggcct tctccaggag aaggccttga gtgttttctc cctctccctg
      841 tgggagaggg tcggggtgag ggcatcagcg cgcacttaac ccgcaactgc aatacgtttc
      901 atatccgtca tatagccgcg cagtttctta cctacctgct caatcgcatg gcagcgaatc
      961 gcttcgttca catcacgcag ttgcgcgtta tctaccgcac cttccggaat agctttaccc
     1021 agatcgcccg gttgcagctc tgccataaac ggtttcagca gcggcacaca agcgtaagag
     1081 aacagatagt taccgtactc ggcggtatca gagataacca cgttcatttc gtacagacgc
     1141 ttacgggcga tggtgttggc aatcagcggc agttcgtgca gtgattcata gtaagcagat
     1201 tcttcgatga tgccggaatc aaccatggtt tcaaacgcca gctcaacgcc cgctttcacc
     1261 atcgcgatca tcagtacgcc tttatcgaag tactcctgct cgccgatttt gccttcatac
     1321 tgcggcgcgg tttcgaaggc ggttttgccg gtctcttcac gccaggtcag cagtttctta
     1381 tcatcgttgg cccagtccgc catcataccg gaggagaatt cgccggagat gatgtcgtcc
     1441 atatgtttct ggaacagggg tgccatgatc tctttcagct gttcagacag cgcgtaagca
     1501 cgcagtttcg ccgggttaga gagacggtcc atcatcaggg tgatgccgcc ctgcttcagc
     1561 gcttcggtga tggtttccca accgaactga attagttttt ctgcgtatgc cggatcggtg
     1621 ccctcttcca ccagcttgtc gaagcacagc agagagccag cctgcaacat accgcacagg
     1681 atggtttgct cacccatcag gtcagatttc acttccgcaa cgaaggacga ttccagcacg
     1741 cccgcacggt gaccaccggt tgctgccgcc catgctttag cgatcgccat gccttcgcct
     1801 ttcggatcgt tttccgggtg aacggcaatc agcgtcggta cgccgaatcc acgtttgtac
     1861 tcttcacgta cttcggtgcc agggcatttc ggcgcaacca tgactacggt aatgtcttta
     1921 cggatctgct cgcccacttc gacgatgttg aaaccgtgcg agtaacccag cgccgcgccg
     1981 tctttcatca gtggctgtac ggtgcgcact acatcagagt gctgcttgtc tggcgtcagg
     2041 ttaaccacca gatccgcctg cgggatcagt tcttcgtaag taccaacttt aaaaccgttt
     2101 tcggtcgctt tacgccagga cgcgcgcttc tcggcaatcg cttctttacg cagagcgtag
     2161 gagatatcga gaccagaatc acgcatgttc aggccctggt tcagcccctg tgcgccacag
     2221 ccgacgataa ccactttttt accctgaagg tagctcgcgc cgtcggcgaa ttcgtcgcgc
     2281 cccataaagc gacatttgcc cagctgtgcc agctgctggc gcaggttcag tgtattgaag
     2341 tagttagcca tggtgattcc tcgtgatgtt gtgcttctta ttgttcggtt cgcgtttgcg
     2401 agattgaatt cactatatga caggaaattt attgcggaaa ttgatatatt cacaacgtca
     2461 cattgcaatt tctgcaacgt caacatcgag ggctgcgcca ttggatttac gcgatctgaa
     2521 aaccttcctg catctggcgg aaagccgcca ttttggccgc agcgcgcggg cgatgcacgt
     2581 tagcccatcc acgctctcac ggcagattca gcgcctggaa gaagatcttg gtcagccgct
     2641 atttgtgcgc gataaccgca cggtaacgct gacggaagcg ggcgaagagc tgcgcgtttt
     2701 cgcccagcaa acgctgttgc agtatcagca attgcgccac accatcgatc agcaagggcc
     2761 gtcgctctct ggcgaattac atatcttctg ctcggtgacc gctgcctaca gccatctgcc
     2821 gccgattctg gatcgcttcc gcgcggaaca cccgtcggtg gagatcaaac tcaccactgg
     2881 tgatgcggca gatgcaatgg aaaaagtggt cacgggagaa gcggatctgg cgattgccgg
     2941 gaaaccggag acgctacccg gcgctgtggc gttttcgatg ctggagaatc tggcagtagt
     3001 gctgattgcc cccgcgctgc cctgcccggt gcgtaatcag gtatctgtcg aaaagcccga
     3061 ctggtcaaca gtgccgttta ttatggccga tcaggggccg gtacgccgcc gcattgaact
     3121 gtggtttcga cgcaataaaa tcagcaaccc gatgatttac gccacggttg gcgggcatga
     3181 agcgatggta tcgatggtgg cgctcggctg tggtgtggcg ctgttgccgg aagtggtgct
     3241 ggaaaacagc ccggagcctg tacgcaaccg ggtaatgatt ttagatcgca gcgatgagaa
     3301 aacgccgttt gaactgggcg tgtgtgcaca aaaaaagcgg ctgcatgagc cgctgattga
     3361 ggcgttctgg aggattttgc cgaaccacaa atgacgttgt cgcgcgggta ggcctgataa
     3421 gcgaagcgct atcaggcatt tttccctaac ccgccaaaaa gaacctgaac gccggattat
     3481 tggtttcgtc gtggcaatcg tagcccagct catttagccg ggtttcgaaa tccggttcat
     3541 ggtcgccgag ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt
     3601 ggaacaaaga aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg
     3661 gtgattccgg gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc
     3721 cgaccatata gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt
     3781 agccgccgtc gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc
     3841 gcacgccgac aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga
     3901 ccgatcgccc gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg
     3961 tcaccgccaa caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca
     4021 ggccgtggaa gttcacgttg gcaccggaaa gaatatgcgc caggcgttcg ccgcgaatgt
     4081 tgtgctgagc gatatatttt ttcattcccg ccagcgccaa cgcgccagag ggttccgcca
     4141 ctgcgcgcac atcttcgaat aaatccttca tcgccgcaca gatcgcatca ctatcgacgg
     4201 tgatgatgtc gtcgagatac tcctggcata aacggaaggt ttcgtcgccg atgcgtttta
     4261 ccgcgacacc ttcggcgaac agtcccacgc gcggcagatc aaccggatga cccgcatcca
     4321 gcgctgcttt caggcaggcg gagtcttccg cttctacggc gatcactttg atttgtggca
     4381 tcagttgttt gatcagcacc gcaacgcccg ccgccagacc gccgccgccg actggcacaa
     4441 atacgcggtc gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg
     4501 caatcaccat cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt
     4561 cgatcgcttt agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga
     4621 atccacgtac cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct
     4681 tcacgcctaa ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag
     4741 tgatcacgcc gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc
     4801 gcagcttaaa gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa
     4861 gacgcgacga cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg
     4921 gcgcgcgcag cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg
     4981 agtcagccat tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct
     5041 ggttgccagg ctggcgtagg cgcgcagggc aaaggaaacc tgacgttcac ggtttttcgg
     5101 cgtccaggct ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc
     5161 gcttacctgt aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc
     5221 aatcagacca atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc
     5281 agaggtgccg ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat
     5341 tgatttcagg aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc
     5401 ttcatagcgg atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac
     5461 cgcgtcgtcc tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc
     5521 gacgcccgcg gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag
     5581 gccgccgtct ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc
     5641 atcgagagaa tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg
     5701 gcctgcgcgg aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc
     5761 cagagtttgc ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc
     5821 gcgatccagt tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg
     5881 gtatttttgg gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc
     5941 gatatcactc atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac
     6001 cgtgttggta gagccgccca tcgcgatatc cagcgtcatg gcattttcaa acgccgcttt
     6061 gctagcaata ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa
     6121 ttcaacaatg cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag
     6181 cagcgagccg ttgcctggct gcgacaaacc cagcgcttcg gtcaggcagt tcattgagtt
     6241 agcggtaaac atcccggagc aggaaccgca ggttgggcag gcggaacgtt caacctgatc
     6301 gctctgggag tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag
     6361 cttgatgatc tgctcggaca gtttggtttt cccggcttcc atcgggccgc cggaaacaaa
     6421 gatcaccgga atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca
     6481 gttagagatg cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc
     6541 agcgatcagt tcgcgagatg gcagtgaata aagcatcccc ccgtggccca tagcaatccc
     6601 atcatccacc gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc
     6661 ggcgaccagt ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga
     6721 gttcacaacc gcgataatcg gcttgccaaa atcggcatcg gtcattccgg tggcgcgcca
     6781 cagcgcacga gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg
     6841 catactttat ttactcccag tgtctgtctc gtaaatggga cggtaagtgc cgtcccattt
     6901 tttgtattta ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga
     6961 agaggccgaa gaaggcttgc tgaatgcatt tggtaaccgg gccgcaacgg ccttcaccca
     7021 cctgaatacc gtctacactg cgcactggcg tgatttctgc cgccgtaccg gacataaaca
     7081 cttcatccgc caggtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca
     7141 gctctttcgc cagtttgatg atggcatcac gggtaatacc cggcagcgcg gatgaggtaa
     7201 acggtggggt gaacagcacg ccgtctttca cttcaaacag gttttcacct gcgccttcag
     7261 agatgtaacc gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc
     7321 tacccaccag cagggaagag aggtagttac caccggcttt tgccgccgtc gggatggtgt
     7381 ttggtgctgc gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg
     7441 cgcccagata cgctccccac gggaaagcag cgataatcac gtcggttgag tatcccgctg
     7501 gcgggtttac tcccatgcca acatcaccga cgaagatcag cggacggata taggcgctgg
     7561 tgagattatt tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgcg
     7621 aaaccgggaa gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc
     7681 ggaatacaac cggtcctttg tgcgagtcgt agcaacggat gccttcaaaa accgaagtgc
     7741 catagtgcag cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc
     7801 cattgaacca aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag
     7861 gcgcggattt gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc
     7921 agtttattta actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg
     7981 atatttatat tttgtgcatc gctggcggcg gccatattca ttgagcagac gtggaaacca
     8041 cgatgacgca ccacgcgtaa aacacgttct aaggtttctg gattgaagcg agccgataca
     8101 ttgacctgat gttgcatcat gataatttct ccaacatttc tgaattactg gcgccaggcg
     8161 gcaccagcgg ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat
     8221 cactgttcag catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc
     8281 cagggatgcc gaaggcgctg gctaacatga ggaaatcggg gttatcagta agggtggttt
     8341 cgctgtatcg ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt
     8401 tatcgagtaa gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca
     8461 cattcatcat gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga
     8521 cttgtgcgcc aaccgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga
     8581 tgaaattttc cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca
     8641 catctgtggt cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg
     8701 gcgcgtagat agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg
     8761 cgcagtattg ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat
     8821 ttaaatcacc ctgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga
     8881 tatccatatg gataacactg gcgtgtggtg cgaaagtatt cagtttgccg gtcacccggt
     8941 catcaaaacg tgcgcccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg
     9001 ctttgatgcc gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc
     9061 ccagcccttt cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag
     9121 ccggaactgc ctgcgccata cccacgccac cgccaacgta cagcatcggt ttttgcgctt
     9181 ttgccagcat ctggcgcgct tgctcaactt cggcatgtgg gaaagtcact tcgttttcaa
     9241 cggtggtgaa ccacggttcc aggtcaccgc tggctaactg gatatctttt gggatatcga
     9301 ccagaaccgg accaggacga cctgagcagg caacgtcgaa tgcttcagcc atgatgcgcg
     9361 gcaactcttc cagcgactgc accagaaagc tgtgcttggt acaggctaac gacaatccta
     9421 ggacatccac ttcctgaaat gcgtcagtgc cgataaacgg tgcggacact tgaccggtga
     9481 tggcaacaac agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg
     9541 cgcccggacc agacgtggcg atacatacgc cagttttgcc ggtagcacgg gcataaccga
     9601 tagccgccat tgccgcaccc tgctcatgtc ggcatagcaa gtgctccacg ccgccgtcat
     9661 acaatgcatc gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac
     9721 cctgtgcccg caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct
     9781 gaatcttgag aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt
     9841 aaggtcaaaa aaaacccccg gacctttcgg tgcgggggtc ttagttcgtt aaggcttgat
     9901 ctctaagcct ttcctcgtcc aagtgcagcc ccgcacggtg ggataataat caccaccacg
     9961 ctaatcacga ctaggctaat cactcgtaga agggctgtca ttttgtcttt tcttgcatct
    10021 tgttcgaagg aatgcctaaa gagttaccat agattttgcg aatagcacaa gatatttttg
    10081 ggcctatttt tatgatagct ttaataattt caatagaagt tgttgttttt taaaaagaaa
    10141 atagtgagtg aaaaaaattc aatttcagta taaatgttgt tcccttcctc gtagttcatc
    10201 ctttgtccgc tgcatttttg cgagcatcat tccaccgcca gaacaactca acttcacttg
    10261 cacaaataaa caggaatcag ccactaaaat tcacgaatta gcgggctaca gcagcgatat
    10321 cttatgcaca taatggtgac tcaaggaggg cttatgtcac tgtcaattgt tcatacccgc
    10381 gcagccctgg gagtaaatgc gcccccgatc actgttgagg tacatatcag taaaggtcta
    10441 cccggcttaa cgatggtggg cttaccagaa acaactgtaa aagaagctcg cgatcgcgtg
    10501 cgcagcgcca ttatcaatag cggatatgaa tatccggcga aaaaaatcac catcaacctt
    10561 gctccagccg atctgccaaa agaaggggga cgatatgatt tacctatcgc cattgcgttg
    10621 ctggcggcct cagaacagct tacagccaat aagttagatg aatatgaatt agtcggagaa
    10681 ctggctctta caggcgctct gcgtggcgtt cccggtgcaa tctccagtgc aactgaagct
    10741 attaagtcgg gcagaaaaat tatcgtcgcg aaagataacg aagatgaagt ggggctaatt
    10801 aacggtgaag gatgcctgat agccgatcat ctgcaagctg tctgtgcgtt tctggaaggt
    10861 aagcacgctc tcgaacgccc gaaaccaact gatgcagtat cccgggcgct acaacatgat
    10921 ctcagtgatg ttgtcggtca ggaacaagga aagcgaggac tggaaattac cgccgctggc
    10981 aggcacaacc ttttactgat tgggccgccg ggaacaggta aaacaatgct cgccagccgt
    11041 attaatggcc tgttgccaga tttaagcaat gaagaggcac tggagagcgc tgcgatatta
    11101 agtctggtaa atgctgaatc agtacaaaaa caatggcggc agcgcccgtt ccgctcacct
    11161 catcacagtg cgtcgttaac tgcgatggtg ggcggtggtg caattccagg gccaggtgaa
    11221 atttcgttgg cgcataacgg cg
//
LOCUS       DAKISA010000132        11220 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.132, whole genome shotgun sequence.
ACCESSION   DAKISA010000132 DAKISA010000000
VERSION     DAKISA010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11220
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.132"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            564..911
                     /locus_tag="OVW45_003709"
     CDS             564..911
                     /locus_tag="OVW45_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCV5528060.1"
                     /translation="MILDSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFI
                     GSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEI
                     GAVPAIPQQSSLF"
     gene            964..1359
                     /locus_tag="OVW45_003710"
     CDS             964..1359
                     /locus_tag="OVW45_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCV5528061.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            1400..2143
                     /gene="cmoA"
                     /locus_tag="OVW45_003711"
     CDS             1400..2143
                     /gene="cmoA"
                     /locus_tag="OVW45_003711"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCV5528062.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCSRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            2140..3111
                     /gene="cmoB"
                     /locus_tag="OVW45_003712"
     CDS             2140..3111
                     /gene="cmoB"
                     /locus_tag="OVW45_003712"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCV5528063.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(3276..5705)
                     /gene="torZ"
                     /locus_tag="OVW45_003713"
     CDS             complement(3276..5705)
                     /gene="torZ"
                     /locus_tag="OVW45_003713"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCV5528064.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKDGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTTRIQHPMVRKSYLDNPLQPT
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIDFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLRGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMRAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5730..6830)
                     /locus_tag="OVW45_003714"
     CDS             complement(5730..6830)
                     /locus_tag="OVW45_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCV5528065.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPGLADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(7218..7964)
                     /gene="cutC"
                     /locus_tag="OVW45_003715"
     CDS             complement(7218..7964)
                     /gene="cutC"
                     /locus_tag="OVW45_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCV5528066.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7978..8544)
                     /locus_tag="OVW45_003716"
     CDS             complement(7978..8544)
                     /locus_tag="OVW45_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCV5528067.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLLGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8760..10496
                     /gene="argS"
                     /locus_tag="OVW45_003717"
     CDS             8760..10496
                     /gene="argS"
                     /locus_tag="OVW45_003717"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCV5528068.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRHSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGDEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKL
                     QSGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAK
                     GLAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRV
                     LYYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLA
                     DLLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNM
                     LAFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETL
                     TVVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLG
                     LDTLGIETVERM"
     gene            10673..11161
                     /locus_tag="OVW45_003718"
     CDS             10673..11161
                     /locus_tag="OVW45_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HCV5528069.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIENDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
BASE COUNT         2777 a         2814 c         2661 g         2968 t
ORIGIN      
        1 aagttagtct cataagttta gctgtctcat aatccaaaaa caaccttttg agactacttg
       61 agggtgcatt ctaccgttac gttgaaagtg tctcaaaagg acttccttat gagactggtt
      121 aatagagcgc tatgaagatt caggagggta actataacca tcactttcta actttcacgt
      181 aggaaatcgt taagtttctt gatagttaca ccatgtgacc aactatctat atatcgttat
      241 atagtcctgt atatagcact atatgtagtg tctggagagg gacgactaac acgatacagt
      301 ttaccctgaa aagagacttc acagattctt tatatatttc aataagttat gctaaaaaga
      361 gactataccg atactgcata gaatttgtgt aggattgcat agatacttgg aaaaacaccg
      421 ttaaattgca tagtcattca cctgatattg tcctgttttt acaggtagtt gattcggatg
      481 ttctaggaat catcctcata gttgattcgg ttagttgggc ttgtaccaac ccctaatcaa
      541 cctcatagtg tatttagacc ttgatgattt tagacagccg ccctgttcat gcagcacgtc
      601 cacacagtga agctattcgc gacgctcaac gaaaaaaacc taaagttccg gtacatgctg
      661 tactgacggc gacaaatccg ctgattcgtt ttatcggtag tgatgatatg acgcaaaacc
      721 gggaattatt tcaggtctgg ttacagaaat tagcgcagtg gcatcagacc actacgcctt
      781 atcttttttt acatacgcca gacattgccc aggccccgga actggtacat accctgtggg
      841 aagacttacg taaaacgctt ccagagatcg gagcagttcc ggctattcca cagcaatctt
      901 ctcttttctg aatttgccac ctatcataga caggtgccat cggccatttt aaagggagtt
      961 tgtatggtaa gcgcgctata tgccgtttta agtgcgttgt tattaatgaa gttctctttt
     1021 gatgtcgttc gcctgcgaat gcagtaccgc gttgcctatg gtgacggtgg ttttagcgaa
     1081 ctgcaaagcg ctattcgcat tcatggtaac gcggtggaat atattcccat cgcgattgtg
     1141 ctgatgctgt ttatggaaat gaatggcgca gaaacctgga tggtgcatat ttgcggcatc
     1201 gttttgcttg ctggtcgtct gatgcattat tacggttttc atcaccgtct gtttcgctgg
     1261 cgacgttccg gcatgagcgc cacctggtgt gcgctgttgc tgatggtgct ggcgaatctt
     1321 tggtatatgc cctgggagtt ggttttctcc ctgcgttagc gcacaatacg ccactttctt
     1381 tttcccggat ttttacgtta tgtctcaccg cgacacgcta ttttctgccc ctatcgccag
     1441 actgggcgac tggacctttg atgaacgggt agctgaagtc ttcccggata tgatccagcg
     1501 ttccgttccc ggctattcca atattatttc catgattggt atgttagccg agcgcttcgt
     1561 tcaacctggt acgcaggttt acgatctggg ttgttctctg ggcgcggcga cgctctcggt
     1621 gcgtcgcaac attcatcatg ataattgcaa aattattgcc atcgacaact ccccggcgat
     1681 gattgaacgc tgcagtcgtc atattgacgc ctataaagcc cctacgccag tagacgtcat
     1741 tgaaggtgat attcgcgata tcgccattga aaacgcatcg atggtggtgc tgaattttac
     1801 cctgcaattc ctggaacctt ccgagcgcca ggcattactg gataaaattt atcaaggact
     1861 gaacccgggc ggcgcgctgg tgctttcgga aaaattcagt ttcgaagatg ccaaagttgg
     1921 tgaactgttg ttcaacatgc accacgactt taaacgtgcc aacggttaca gcgaactgga
     1981 gatcagccag aagcgcagca tgctggaaaa cgtgatgctg accgattccg tggaaaccca
     2041 taaagcacgc ctgcataaag ccggttttga gcatagcgag ctgtggttcc agtgctttaa
     2101 ctttggttca ctggtggcat taaaagcaga ggacgctgca tgatcgactt tggtaacttt
     2161 tattctctga ttgccaaaaa tcatctttca cactggctcg aaacgctgcc cgcgcagatt
     2221 gctaactggc agcgcgagca gcagcacggg ctgtttaagc agtggtccaa cgcggtggaa
     2281 tttctgcctg aaattaaacc gtatcgtctg gatttattgc atagcgtaac cgccgaaagc
     2341 gaagagccac tgagcaccgg gcaaattaaa cgcattgaaa cgcttatgcg gaacctgatg
     2401 ccatggcgca aagggccgtt ctcactgtat ggcgtcaaca tcgataccga atggcgttcc
     2461 gactggaaat gggatcgtgt tctgccccat ctttctgatt taaccggacg caccattctg
     2521 gatgtcggct gcggcagcgg ttatcacatg tggcgcatga ttggcgcagg cgcgcacctc
     2581 gcggtgggga tcgaccctac gcaactgttt ctgtgccagt ttgaagcggt gcgtaaacta
     2641 ctgggtaacg atcagcgcgc acatttgtta ccgttaggta ttgaacagct tccggcactg
     2701 aaagcctttg ataccgtctt ttcgatgggc gtgctttacc atcgccgttc gccgctggag
     2761 catctctggc agttgaaaga tcaactggtg aatgagggtg aactggtgct ggaaacgctg
     2821 gttattgatg gcgatgagaa cacggtgctg gtaccaggcg atcgttacgc gcaaatgcgt
     2881 aatgtctatt tcatcccttc cgcgctggcg ctgaaaaact ggctgaagaa gtgtggtttt
     2941 gtagatatcc gcattgtgga tgtgtgcgtt accactactg aagagcagcg acgcactgaa
     3001 tggatggtta ccgaatctct gtccgatttc ctcgacccac atgatccgag taagacagtt
     3061 gaaggttatc ctgcgcctaa acgcgcggtg ctgattgcgc gcaagccgta aaggtctggt
     3121 attactgccg gatgcggcgt gaatgcctta tccggcctac aaacccttgc taattcaatc
     3181 tattgcaggg actatgtagg cctgataagc atagcgcatc aggcagtttt acgtttgcat
     3241 aacctcagcg cccgtttccg ggcgctattc acttcttacg cctgaacagc aggcttatca
     3301 aatgccgtta acttcggtgc gttacccgta tatttttcga tatacaccag cgcagagtta
     3361 ccggcacagg catttgccag ctgcgagctg gggatatccg ccgttaacac gttcgcactg
     3421 ccgtttttac acaagccgtt ttccagatct ggccatgcac cttcatgcac gcataccacg
     3481 ccttttttga tcccgtcagt caccaccgcg cctgtcaaaa tctgaccacg tttgttccag
     3541 acacgcacca gatcgccatt cgcaatacca aatcgagcag catcttcggt gtgaatagtg
     3601 atcggttcac gatcggcaac cgcatatttt ttacgcagtt ccgcatagtt aagctgactg
     3661 tgtaaacggt gtgccggatg tgcggtcaga agctgcaact gcttctcgtc ggcggtaccc
     3721 ttccactcat caggcgcaag ccaggttggg tgtgccgggc aatccttata gccaaatttt
     3781 tccagcgttt tggagtaaat ctcaattttg ccacttggcg tacccagcgc atttttcacc
     3841 ggatcggcgc ggaaatcacc ataacgaacg tactgttcgt tcttctcgct gcggcgcatt
     3901 tcgatcagtt tattttgctg ccagaaggca ttaaacattg gcatagtgac gcgttgcgca
     3961 cgggcccctt tctgagcggc atcatagaaa aatttcagcc acgccatttc atctttacct
     4021 tcggtataga tctcttttcc gccaggtttg agtaattccg caagatcggc aaacacatca
     4081 aaatcgtttc gcgcttcaaa ttgcggagca acagcctgct tcatcggcac aatatgctgg
     4141 ttgctgtaat caccggtcat cgtcaagtca ttgcgctcaa acgatgtggt gatcggtaat
     4201 acgatatctg catgtttagc agctgcggtc cagtagcatt cagaaacgac gatcatctcc
     4261 ggtttctgcc atgctttaat cagacgatta gtatcctggt gatgggtaaa gttaccgcca
     4321 cccgcccacc agatcatttt gatattcgga tacgtttgct ctttaccgtt atgctgatat
     4381 ttgccgccag gattttccag cgcatcgacg atacgggcaa caggaatcgc cctcattccg
     4441 ccatcatcag cggcctcgct ggcctgtccg gcaatcgccg cagacatttc cggcagcacg
     4501 ccgccaacac gtgtcgggtt gccaccattg gagtaatgat aagagaaacc aaaaccgccg
     4561 cccggtgtac caatctgccc taacatcgcc gccagcgtca ccagcatcca gtgtttctgt
     4621 tcaccatatt gctggcgctg aattccccag cctgccatca gcattgtgcg attcgccgcc
     4681 atcagttcag cgagtttgac tatctgcgct tccggtacgc cagtaatttc cgccgcccag
     4741 gccgcgctct tcggcgtatt atcgctctta cctctcagat actcttcaaa ctgcggatac
     4801 ccggtagtgt atttttcaag gaacacttta tcgtgtttgc cttgtgtcat cagggtatgc
     4861 gcaatcccta acatcagtgc cacgtcggtg cccatattcg gcgcgatcca ggtggcatta
     4921 tcgccaaaga agtcgatggt ttcggagcgg ataggatcaa tggcaatcac tggtttgcca
     4981 gattttttca gctgatggaa gtattccagc ccttgctcat cggtactgct ccaggcaatt
     5041 tttaaggtat tcagcgggtt catcccccac agcaccacaa cctggctgtt ttccagaatc
     5101 agcggccagg aggtctgctg ttcatacacc tctacagaac cgaccacatg cggcatgatc
     5161 acctgtgccg ccccggttga ataatcgccg ctatgcccgg aataaccgcc cgccaggttc
     5221 atataacgtt gcagtaaggt ttgcgcttta tgcaacacgc cagaagagcg ccagccgtat
     5281 gatccggcaa aaatagccga tggtccgtta gctttgcgaa tgcgatcgtg ttgctcatga
     5341 atcagtttta atgcttgttc ccagctcacc tgtacatagg tatcttcgcc acgacctttc
     5401 gtcggttgca gtggattatc gagatagctt tttctcacca tcggatgctg aatacgcgtc
     5461 gtggtgtgta cctgatccgc cgccgtagac tgtaaggaat tcggtatggt tttcgccagc
     5521 gcgcctgttg aagaaacaat cttgccgtcc ttcacttcta cgttcatcgc tccccaacgt
     5581 cccgcggtga ggattttacc gtccttctct tctgcccatg cggggagcgg tgctgccgat
     5641 gtcaccacca gcgctccagc ggcaataccg ctgtgtttaa taaattcacg tcttgttaat
     5701 gtcataactt cctccctgat caacgaggat cactgtttct cggtaatatc tttggcgttg
     5761 tactggaaat accgcgttaa aatgtccagt tcgttttcgc tcatgctggt tcgtgccccc
     5821 attcctttgg caatggacgg ccacgcattg acggtgtaat ggtcggcggc aataggggca
     5881 tgacaaccag cgcaataggt atcgtcaagt ttttcagcgt attgccatag cggtttacgg
     5941 tccgctaatc cgggatcggt aagcgcaccc tgtaaagacg cctgacgcca ttgattgccg
     6001 tattcgtcag cctgccattc cccgtttacc gtgagcgcct tgataccttc ttcacttaat
     6061 gtggctagcg ccagccgttg acctgccgcc aggtagagcg tgttttcact gccctgcatt
     6121 tgataaccct gcaacagaac gatcggctgt ttgccactgg catcaacgac ggtgagatcg
     6181 gtaccaggat tcacggtagc cagctcgcct atgcgagaag ttttgaaagg ataaatatgt
     6241 gcgccattag tcactgaagt agcggcctga ctttccagct catgcgccgc gttgtcatcc
     6301 atttttattt ctggcggaaa atgggcaatg cctttatgac aatcgataca ggtttcgccg
     6361 cccttttgtg ctttgttatg cattttctgc gcagattcac tttgcgaggc aatatccatg
     6421 gcatcaaaag aatggcaact acggcacgtt gcagagtcag ttgcttttaa ttctttccat
     6481 actgtttcgg ccatttcctg gcgatgagtt tcgaacttat cgtcactgtc tattttgccg
     6541 ctaacaaatt catgataaat atctttagat gcttttaatt tagcaaataa ataatccatc
     6601 cctgactttg gaatatggca atctgcacat tccgcacgta tccctttctg gttcgaaaag
     6661 tggacagttc cctgatattc ctcaaaaggt ttactcatcg agtggcagga aaggcaaaat
     6721 gctgtatccg acgttttatg taaggctttt tgcgccagca ataacccgcc gccaccaacc
     6781 acaaccgcta tcagcaaaaa caataaccca atgcgttttt tccctcgcat agattcctct
     6841 aaataaattc cagaacaccg aaatcattgt ataattaaat acacaacgaa ttaaccgctt
     6901 gcataactcg gcacaacact gcctgaaata atcgataaag aatatgattt attacaatgt
     6961 aatcattaat tgctaaggaa taacccagtt gctatttaga atatatattt atcagtttta
     7021 gcaatttaaa tcccataatc aatgtgaata taaaaaatat tattagcaca acgctatgtt
     7081 cactgtcacc cgctcaacag atcacggtta tcgttcgttt tttatactgc tcagggataa
     7141 aaaaaggccc ctgttgaaat tgcaggggcc tggtacgagc aagcatcata ttgggcgaca
     7201 tgatgcaacg gtaaaaatca tttggcctga tggcgttcaa tgattccttt catttcagca
     7261 accgccgccc cgtctacgat ataacgcgaa tactcgtccg cgtgttcatc tgatgacatg
     7321 gacaatcctt gattacgata acgcatcggt gaggcttgcc acgctcccgc ggagctatgg
     7381 acttccagca ctccggcatc gaggaagtgg tgcaggtttt ctgcacggac tcctgctccg
     7441 gccataatga ttggagcatc acgatgggca ataagttcca taatttttga taaaccttgc
     7501 agtgcgtctg atttttgccc tgatgtcagt acccgggcaa tgcccaattc cgcaagatta
     7561 ttgagtgtat ataaagggtt agcgcacata tcgaaggcgc gatgaaaagt cactgccagc
     7621 ggaccggcag cagccattat tttttccatt cgtggcatat cgacattccc gtcaacatcg
     7681 agaacgcccg tcaccagtcc aggaaaacct aattcgcgga ccgtgcgcac atcctcaaga
     7741 atggcggcaa actcaccgtc gctgtagcaa aaatcaccac cgcgtgggcg aattatcgga
     7801 tgcacaggga tcgtcacccg ctggcgcacg gatttcagta cccccaacga cggcgttaag
     7861 cccccctctt ttggggctgc gcataattca actctgtctg cgccgttttg ctgcgccgtt
     7921 agtgcacatt ccatgctgta acagcaaatt tccagtaatg ccatttttgc tccttaatta
     7981 cgctgactgc tcgctggcaa cgatctcttc aatggaccac ggatgaaact taatagttgt
     8041 tttgccatcg gtgaccgcca gcgtcggatt cggtaatcgc tcatgctcac cttttggtga
     8101 actggtttta actgagatcc ccggtaggct gagtccttca tcagaaagca atgccagcgc
     8161 acgggcattc agcgtttctg gatcacccag cagaacgatt tcaatatgtt cccaaccttc
     8221 gtgtgggtaa cgtttttccc cgggccacgg tagctccaca atagaaaact gccagtgcgc
     8281 aacctgtacc ggttcatgca atttaaacag acaaatcggt ctgccattga tcatattttc
     8341 tgacaaaagc tcgccacact gttcaaaccc gcgacgccag cgttcagcag tggcgttttg
     8401 atggcaacgc aaagaaatgt gatcggcagt cagcggagtg atattcaacc ccagacggag
     8461 ggaaagttca tctaatgcgt gggtaaatcg cggtaaatcc gatgcaatat cctgcagctc
     8521 gtcgatagat tgccagttcg ccataatcac tcttcgtctt tcagtaaaag cgttaattta
     8581 ccctgttgcc ctgcgccaac caaccgctga tttcacgccg cttctgatgc aatagtgaaa
     8641 acggcaatac gccacgcgca cgttgctgac gaaaacagcc atttgcagta tactcccgcc
     8701 ctaatttctt taactggtgc gggcaatttt tgctcgcttc atcaatgtaa ggtattccgg
     8761 tgaatattca ggctcttctc tcagaaaaag tccgtcaggc catgattgcg gcaggcgcgc
     8821 ctgcggattg cgaaccgcag gttcgtcatt cagcaaaagt tcagttcggc gactatcagg
     8881 ctaacggcat gatggcagtt gctaaaaaac tgggtatggc accgcgacaa ttagcagagc
     8941 aggtgctgac tcatctggat cttaacggta tcgccagcaa agttgagatc gccggtccag
     9001 gctttatcaa cattttcctt gatccggcat tcctggctga acatgttcag caggcgctgg
     9061 cgtccgatcg tctcggtgtt tctacgccag aaaaacagac cattgtggtt gactactctg
     9121 cgccaaacgt ggcgaaagag atgcatgtcg gtcacctgcg ctctaccatt attggtgacg
     9181 cagcagtgcg tactctggag ttcctcggtc acaaagtgat tcgcgcaaac cacgtcggcg
     9241 actggggcac tcagttcggt atgctgattg catggctgga aaagcagcag caggaaaacg
     9301 ccggtgatga aatggagctg gctgaccttg aaggtttcta ccgcgatgcg aaaaagcatt
     9361 acgacgaaga tgaagagttt gccgagcgtg cacgtaacta cgtggtaaaa ctgcaaagcg
     9421 gtgacgaata tttccgcgag atgtggcgca aactggtcga catcaccatg acgcagaacc
     9481 agatcaccta cgatcgtctc aacgtgacgc tgacccgtga tgacgtgatg ggcgaaagcc
     9541 tctacaaccc aatgctgcca ggaattgtgg cggatctcaa agccaaaggt ctggcagtag
     9601 aaagcgaagg ggcgaccgtc gtattccttg atgagtttaa aaacaaggaa ggcgaaccga
     9661 tgggcgtgat cattcagaag aaagatggcg gctatctcta caccaccact gatatcgcct
     9721 gtgcgaaata tcgttatgaa acactgcatg ccgatcgcgt gctgtattac atcgactccc
     9781 gtcagcatca acacctgatg caggcatggg cgatcgtccg taaagcaggc tatgtaccgg
     9841 aatccgtacc gctggaacac cacatgttcg gcatgatgct gggtaaagac ggcaaaccgt
     9901 tcaaaacccg cgcgggtggt acagtgaaac tggccgatct gctggatgaa gccctggaac
     9961 gtgcacgccg tctggtggca gaaaagaacc cggatatgcc agctgacgag ctggaaaaac
    10021 tggctaacgc ggttggtatt ggtgcggtga aatatgcgga tctctccaaa aaccgcacca
    10081 cggactacat cttcgactgg gacaacatgc tggcgtttga gggtaatacc gcgccataca
    10141 tgcagtatgc ttacacgcgt gtattgtccg tgttccgtaa ggcagaaatt aacgaagagc
    10201 aactggctgc agctccggta atcatccgtg aagatcgtga agcgcaactg gcagctcgcc
    10261 tgctgcagtt tgaagaaacc ctcaccgtgg ttgcccgtga aggcacgccg catgtgatgt
    10321 gtgcttacct gtacgatctg gctggtctgt tctctggctt ctacgagcac tgcccgatcc
    10381 tcagcgcaga aaacgaagaa gtgcgtaaca gccgtctgaa actggcacaa ctgacagcga
    10441 agacgctaaa gctgggtctg gatacgctgg gtattgagac agtagagcgt atgtaatcga
    10501 tttttcgtga gagtaaagcc tgatcagggt tacctgatca ggctttttta ttgccatcta
    10561 aatgtattct gaaaatggac atgccattgt tttctgactg ttggataaga ggccagaagc
    10621 gtaatatccg gccccaggga aacgataacg gttgaattta aggaataccg cagtgtttaa
    10681 atttcttgta ttaacattag gcattatctc ttgccaggct tacgcagaag atacggttat
    10741 agaaaacgac catgacattt cagccatcaa agattgttgg caaaaaaatt cagatgatga
    10801 tactgacatt aacgtgatca aatcatgcct gcgacaagaa tacaatctcg tcgatgcgca
    10861 attaaataaa gcctatggtg aagcttatcg ttatatagaa caagtgccac gcacaggtgt
    10921 aaaaaaacct gataccgaac aacttaactt gcttaaaaaa tcacagcgag cctggctgga
    10981 ttttagggac aaagaatgtg aattaatcct ttcaaatgag gacgttcagg atttaagtga
    11041 cccttattct gaatcagaat ggctctcatg tatgatcata cagaccaata cgcgaactcg
    11101 ccagttgcag ctataccgta actctgaaga tttttatcca agccctttga caagaggata
    11161 attcacatct ttttggcatg ttttgttgca agctattcct gataaataat tgcaacaaga
//
LOCUS       DAKISA010000133        10941 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.133, whole genome shotgun sequence.
ACCESSION   DAKISA010000133 DAKISA010000000
VERSION     DAKISA010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10941
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.133"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            110..607
                     /gene="ftnA"
                     /locus_tag="OVW45_003719"
     CDS             110..607
                     /gene="ftnA"
                     /locus_tag="OVW45_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCV5528070.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(645..884)
                     /locus_tag="OVW45_003720"
     CDS             complement(645..884)
                     /locus_tag="OVW45_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCV5528071.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            1075..2286
                     /gene="tyrP"
                     /locus_tag="OVW45_003721"
     CDS             1075..2286
                     /gene="tyrP"
                     /locus_tag="OVW45_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCV5528072.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGV
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2348..3013)
                     /gene="yecA"
                     /locus_tag="OVW45_003722"
     CDS             complement(2348..3013)
                     /gene="yecA"
                     /locus_tag="OVW45_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCV5528073.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTNHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3209..3295)
                     /locus_tag="OVW45_003723"
     tRNA            complement(3209..3295)
                     /locus_tag="OVW45_003723"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3259..3261),aa:Leu,seq:taa)
     gene            complement(3308..3381)
                     /locus_tag="OVW45_003724"
     tRNA            complement(3308..3381)
                     /locus_tag="OVW45_003724"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3347..3349),aa:Cys,seq:gca)
     gene            complement(3436..3511)
                     /locus_tag="OVW45_003725"
     tRNA            complement(3436..3511)
                     /locus_tag="OVW45_003725"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3476..3478),aa:Gly,seq:gcc)
     gene            complement(3663..4211)
                     /gene="pgsA"
                     /locus_tag="OVW45_003726"
     CDS             complement(3663..4211)
                     /gene="pgsA"
                     /locus_tag="OVW45_003726"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCV5528074.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(4268..6100)
                     /gene="uvrC"
                     /locus_tag="OVW45_003727"
     CDS             complement(4268..6100)
                     /gene="uvrC"
                     /locus_tag="OVW45_003727"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCV5528075.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(6097..6753)
                     /gene="uvrY"
                     /locus_tag="OVW45_003728"
     CDS             complement(6097..6753)
                     /gene="uvrY"
                     /locus_tag="OVW45_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCV5528076.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            7049..7225
                     /locus_tag="OVW45_003729"
     CDS             7049..7225
                     /locus_tag="OVW45_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528077.1"
                     /translation="MIKIFIGHYINAFNSIVDVTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            7212..7436
                     /locus_tag="OVW45_003730"
     CDS             7212..7436
                     /locus_tag="OVW45_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCV5528078.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(7503..8225)
                     /gene="sdiA"
                     /locus_tag="OVW45_003731"
     CDS             complement(7503..8225)
                     /gene="sdiA"
                     /locus_tag="OVW45_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HCV5528079.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(8455..9207)
                     /gene="yecC"
                     /locus_tag="OVW45_003732"
     CDS             complement(8455..9207)
                     /gene="yecC"
                     /locus_tag="OVW45_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCV5528080.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(9204..9872)
                     /gene="tcyL"
                     /locus_tag="OVW45_003733"
     CDS             complement(9204..9872)
                     /gene="tcyL"
                     /locus_tag="OVW45_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCV5528081.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(9887..10873)
                     /gene="dcyD"
                     /locus_tag="OVW45_003734"
     CDS             complement(9887..10873)
                     /gene="dcyD"
                     /locus_tag="OVW45_003734"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCV5528082.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGVIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIGCISQKRFKDEGPILFIHTGGAPALFAYHPHV"
BASE COUNT         2714 a         2840 c         2577 g         2810 t
ORIGIN      
        1 ccattcgtat ttttaataat tgaagtttat attttacctt ttgcaaataa taaaataaca
       61 aattatagtg acgccacagc aacaaatata acctttgtgg agcactatca tgctgaaacc
      121 agaaatgatt gaaaaactta atgagcagat gaacctggaa ctgtactctt cactgcttta
      181 tcagcaaatg agcgcctggt gcagctatca taccttcgaa ggtgctgccg cgttcctgcg
      241 ccgtcacgcc caggaagaga tgacgcatat gcagcgtctg tttgattacc tgactgatac
      301 cggcaattta ccgcgtatta ataccgttga atctccgttt gctgaatatt cctcacttga
      361 tgaattattc caggaaacct ataaacacga acaattaatc acccaaaaaa ttaacgaact
      421 ggctcatgct gcaatgacca atcaggacta cccaacattt aatttcctgc agtggtatgt
      481 ttctgagcag catgaagaag agaaactgtt caaatcgatt attgataaat taagcctggc
      541 aggcaaaagc ggcgaaggtc tgtattttat cgacaaagaa ctctctaccc tcgacacaca
      601 aaactaatgc tctccggcgg caggtttact gccgccgttt caaatcagtg acggcaaatc
      661 ttactctgat cggtcgaaaa accgtctttc gaaggtataa atttgccttt tgctgccaga
      721 aacgctacca gctctcccgc tgtcatccct tctgccgagc aggtgtgaaa acgtgcctgt
      781 tcaccaaaac gcgctttaat cgcagcttcc agactggcat gcgtatattg ctcgcctgat
      841 tcaatcatca tatttaacac ttcatgaccg tgaatagagt ccatcgttcc tcctcaaaaa
      901 aagcctagcg tagcgattgc cgcttatgaa gactttgcgc cagcgcagga ctgaatgctt
      961 tttattgtac atttatattt acactatatg taacgtcggt ttgacgaagc agccgttatg
     1021 ccttaacctg cgccgcagat atcactcata aagatcgtca ggacagaaga aagcgtgaaa
     1081 aacagaaccc tgggaagtgt ttttatcgtg gcgggaacca caattggcgc aggcatgctg
     1141 gcaatgccgc tggctgcggc cggtgttggt tttagcgtta cgttaatctt gttgattggg
     1201 ctttgggcgt tgatgtgcta cacggcgcta ttactgctgg aggtgtacca gcatgttccg
     1261 gcagataccg gtctgggcac gctggcaaaa cgctatctgg gacgctacgg tcaatggctg
     1321 acgggcttca gtatgatgtt cttaatgtat gctctgactg cggcatacat cagcggtgtc
     1381 ggtgaattgt tggcctccag catcagcgac tggacaggta tttcaatgtc ggcaaccgct
     1441 ggcgtgctgt tgttcacttt tgttgccggt ggcgtggttt gtgttggaac atcgctggtc
     1501 gatttattta accgttttct gttcagcgcc aaaattattt ttctggtggt gatgctggta
     1561 ttgctgctac cgcatattca caaagtgaat cttttaaccc tgccgttgca acaggggctg
     1621 gctctgtcag caatcccggt gatttttacc tcatttggtt ttcacggtag cgtgccgagt
     1681 attgtcagct atatggatgg caacgttcgt aagctacgct gggtgtttat aaccggtagt
     1741 gcgatccccc tggtggcata tattttctgg caggtggcaa cgcttggcag cattgattca
     1801 acaaccttta tgggattgct ggctaatcat gctggattaa acgggctgtt acaggcgtta
     1861 cgcgaaatgg tggcctctcc gcatgttgag ctggcagtgc atttatttgc tgatttagcc
     1921 ctcgccacgt catttctcgg cgttgcgtta ggcttatttg attatctggc tgatttattt
     1981 cagcgttcaa ataccgttgg tggacggttg caaactggtg caattacctt tctgccgccg
     2041 ttggcgtttg cactgtttta tccacgagga tttgtgatgg cgctgggtta cgccggtgtg
     2101 gcgctggcgg tactggcatt gattatccct tcgctgttga cctggcaaag cagaaagcac
     2161 aatcctcagg cgggttaccg ggtcaaaggt ggtcgtccgg cgctggtggt ggtgtttctc
     2221 tgtggtattg ctgtgattgg cgtgcaattt ttgattgcgg cagggttgtt accagaagtg
     2281 gggtgatcag atagcctcaa attccttatt gggtgccaga attaacgctg acacccaatt
     2341 tggcctctta atgcaggcag cactgcttaa atttcttacc actaccgcac gggcaaggat
     2401 cgttacgccc cggtttctct tctgctttga tcggttgctg aacagctttt tcctgcggat
     2461 gcgccatcca gtacgcatgt agatcaagcg ccgccagtcg aatggcatct acgctctctt
     2521 caaacgcttc tggcgacatc ttttctaccc gctcgaagtt ttcctcagta ccgtgcagcg
     2581 caatcgcctc cagcgctggt tttaacgaat cgggcaacgt tgaccagtca gaaagtgcca
     2641 cgccccgcat atagccaaag caccactcct caacaatcgt cagctcgctg ccatcaactt
     2701 ctcgcaagcc gaataacggc tcaaactgct ccgggaattc gttcagacgc tctgcggtat
     2761 cggccatatg ttgaaaagcc aggttcataa agcgcgtcat ctctttctct gacgcccagc
     2821 gcggcacata gtcagcccca ccccacacgg caaccagcca ctgttccggt tcaatctctt
     2881 gcggagaact caacaccgcc gtcaataaac cgtccagctc cgccacatca aggatggcgt
     2941 ggttagtgtt gtatttggtc agaatatcgt ccagccattc caactcactt tcgtttaacg
     3001 gtcccgtttt catacgcttt tccttgtgga tctcaactcg ccagcaccta tcttacatgc
     3061 cggtccgtat cagagatact ttttgagtgg ctttgccggt gattaaaaat taaggagggt
     3121 gtaacgacaa gttgcaggca caaaaaaacc acccgaaggt ggtttcacga cactgcttat
     3181 tgctttgatt ttattcttat ctttcccatg gtacccggag cgggacttga acccgcacag
     3241 cgcgaacgcc gagggatttt aaatcccttg tgtctaccga ttccaccatc cgggctcggg
     3301 aagaaagtgg aggcgcgttc cggagtcgaa ccggactaga cggatttgca atccgctaca
     3361 taaccgcttt gttaacgcgc caaattcttc aggcctttca gccagacatc cgcttgacgc
     3421 cgatgtcttt taaactggag cgggaaacga gactcgaact cgcgaccccg accttggcaa
     3481 ggtcgtgctc taccaactga gctattcccg cattcatcaa gcaatcagtt aatcacttga
     3541 ttttattatc gtctggcaat cagtgccgcc gttcgatgcg ttgcattcta cttacctggc
     3601 gcgatgagtc aacgatattt ttcaccactt ttgatcgttt gctgaaaatt acgccgaaac
     3661 gatcactgat caagcaaatc tgcacgcgca gcgctcaaat attgcaacat tgaccacaga
     3721 gtcagtaccg cagccacaaa gaaaagtgca ataccggcgt actcaaccca aatgttcgga
     3781 cgccacagca gccatgccaa cgccaccatc tgggcagtgg ttttcacttt cccaatccag
     3841 gagacagcca cgctactgcg tttacccaac tccgccatcc attcgcgtag cgcagaaata
     3901 ataatttcac gggcgatcat cgttgctgcc ggtaaggtca cccaccagct gtggtaatgc
     3961 tcggttacca gcaccatggc gatagccacg agaactttat ctgctacagg gtcaaggaaa
     4021 gcaccaaacc gggtactctg gttccagcgg cgtgccagaa aaccatcgaa ccagtcagtc
     4081 accgccgcga cgcagaaaat gagcgcggcg gcaaacggcg accaggtgac aggcagataa
     4141 aagaccaata caaagaatgg gataaggatg acacggaaca gtgtaagcaa cgtagggata
     4201 ttaaattgca taatgacggg taactatctg ttgtcagtaa gattacccct atgttgctac
     4261 agagacatca atgtttcaac gaccagaaga tcttttctgc cagaccttgc gaaatacccg
     4321 gcacttttgc aatttcctcg acgctggcgt tacgtaaacc ttgcaaaccg cccatatatt
     4381 tcaacaacat ttgccgacgt tttggcccga cgccttcaat ggtttccagg gaactggtat
     4441 ttttgacctt cgcccgtttt ttacggtgcc cgccaatcgc gtgatcatgt gattcatcgc
     4501 gaatatgctg gataacatgc agcgcgggag aatctggcgg caaactgaat ccctcacctt
     4561 ccggctcaaa gaacagcgtt tccagcccag ccttacgatc tgctcctttg gcaacgccaa
     4621 gtagcagcgg atgattttta tcccatgaga catccagttc agcgaagaca tttttcgcct
     4681 gtgcaagctg gcctttgccg ccgtcgataa ggatcacatc cgggatctta ctgtcgtcaa
     4741 tggctttacc ataacgccga cgcagcacct gattcatcgc cgcataatca tcgcccggcg
     4801 tgatgcctgt aatgttatag cgccgatact ccgcacgcag cgggccgtta gcatcaaaca
     4861 ccacacagga agcgacggtt tgttcgccca tggtatggct gatgtcaaag cactccatcc
     4921 gcttcacttc cggcaatttc aacacgctgg caagcgcggt cagtcgctgg tgaacggtag
     4981 attgctgcga aagtttgctg gttaaggccg tcgccgcatt ggtgcgcgcg agtttcagat
     5041 aacgcgccct atcgccgcga ggtttggttt gaacattaat cttgcgtccc gccagttctg
     5101 aaagggaatc ggcgagcagc gttttatcgc taagattaaa atcgagcagg atctcacccg
     5161 gtaaggtgcg catctggctg ccttgtaaat agaactggcc tacgaaggtt tctaccacct
     5221 cgctcagttc cgtaccgcca ggcactttcg ggaaatagct gcggctgccg agcactttgc
     5281 cctgacgaat gaacaatacg tggacacaag ccatgcccgc atcgaacgcc acaccaataa
     5341 cgtcgaggtc gtcgccggta ttggaaacga attgtttttc ggtgacgcgt cgcaccgcct
     5401 gaatttggtc gcgaatacgt gcagcttctt caaactccag attctggctg gcagtttcca
     5461 tacgactaat gagttgcgta agcacctgat catctttgcc agacaaaaac aggcgcacat
     5521 actcgacctg ctgagcgtat tcttcttcac tcaccagtcc ttcaacgcac ggtcccagac
     5581 agcgccctat ctgatattgc agacacggac gcgagcgatt gcgataaaca ctattttcgc
     5641 actggcgaat ggggaaaatc ttttgcagta gcgccagtgt ttcacgtacg gcatagccat
     5701 tcgggaacgg gccgaaatat tcacctttgg catgcttcgc accacgatgc atcgccagac
     5761 gcgggtgggt atcgccactc aggaagataa aaggataaga tttatcatcg cgtagcaaaa
     5821 cgttgtaacg cggctgatag agtttgatgt agttgtgttc cagcaacagc gcttcggttt
     5881 ctgtatgcgt aaccgttaca tcaatttgct ggatctgggc gaccagcgct tcggttttgc
     5941 gcgaagcgag gttgctgcgg aaatagctgg aaagccgttt tttcaggtct ttcgctttgc
     6001 cgacatagat aaccgtacca ccagcatcgt acatgcgata aacgcctggc tggctggtta
     6061 cggtttttaa aaacgctttt gcgtcaaact gatcactcac tgacttgata atgtctccgc
     6121 attacacaga ccatggcgaa ttgccaggtg agtcagctca acatcgccat gaatgtttag
     6181 tttactgaac atacgatagc ggtagctgtt caccgttttc ggactgagat tgagctgttc
     6241 tgagatctca ttgaccttct ggcccttggt gatcatcagc ataatctgca attcacgttc
     6301 agacaaactg gcaaatgggc tttctgtttt ttctggttcg atctggctta acgccatttg
     6361 ttgagcgata tcagaagcaa tgtaacgctg ccctgaatag acagaacgaa tcgcactcac
     6421 gacttcctgc ggagccgcgc ctttgctgag gtagcccgca gcaccggcct gcatgacttt
     6481 cgctggtaaa gggttttctg tatggacggt aagcatgatg attttgacat cagctgtgga
     6541 acgcgcgatt ttacgcgtcg cctcaagacc gccaatgccc ggcatactca tgtccattag
     6601 caccacgtca acggcatttg tccggcacca cttaacggcg tcttcaccgc acgatgcctc
     6661 accgacgact tttataccct ttatatcttc cagaatgcgt cgtatccctg cgcgcaccag
     6721 ttcgtggtca tcaacaagta gaacgttgat caaaggaata tctccagaaa tagggataac
     6781 gctactgata gttagtcatt cgtatattag cggtttttat tgcaactttg aaacgttaaa
     6841 aatggtgggt attcgatttt tctctcgttt ttgtaaattc gattgtccac agttatggtc
     6901 acgcccgtca ccaacggctt ccgggatcgt ttcacagtaa gcccggcgat gaaatgtgac
     6961 aaaaattgac tttattcagc aaaaatgaaa atcagcccgc tgaatatgta acattaatta
     7021 acccgaacaa tacgggcaaa aaacattcat gataaaaata tttattggtc attatattaa
     7081 cgcatttaat agcattgttg acgtcacgct caaaaaacaa ccactgctat ttttagcaaa
     7141 gcttatggta tactccgccg ccttaacatt tttcaccgca aattttcatt gcaacatgac
     7201 gaggaaaata aatgagtacg cctgattttt ctactgccga gaataatcaa gaactggcaa
     7261 atgaagtctc ctgcctgaaa gcgatgctga cgctgatgct acaggcgatg ggacaagctg
     7321 acgcgggccg cgtgatgtta aagatggaaa aacagcttgc gctgatcgaa gacgaaaccc
     7381 aggctgcagt attttccaaa acggttaagc aaattaaaca agcctaccgt cagtaatata
     7441 aaaccggctg agagcgtgcc tttcagccga tttttgcatc tggcacgcag aaagaaaagc
     7501 gatcaaatta agccagtagc ggccgcgtaa caggccacct gggtcttatt tggtgcattg
     7561 atttttttct gcatgttttt ctgatggaaa ttgaccgtat tctcagagat tgacaaaatc
     7621 atcgctatct ctgctgatgt cttcccttcc gccgtccatt tcagaatttc tttttcgcgc
     7681 ttgctgaaat tcatctctgg cgtcatcact atttcatcat ttaaacgcat cagagccatc
     7741 agactttcgc gcaccagtaa ctgcattttt aattgcagtt catcactaag aatgggtatt
     7801 tcgcgcgtgc tgcaacggga aaaggacaaa aagcccagcg cccgatttgg cagcattaaa
     7861 tactgagtga caccgcggcg taaaccatgt gcgtgcgcgg cttcccataa tggctgtgct
     7921 tcgctgaata agtcatcatt ccacattaaa tggccctgac taaagttttc agggttcagc
     7981 acaggatcaa ttgcgagaaa attttttgcc tgataataac taacccacga ctcagggtaa
     8041 ttggtgtaga aagccacttt aggtcgcgtg aatggtaccg ggtggcgaac acataacgaa
     8101 tagtaatcgt actccagctg ctgagcctga agctctattt catggtagac ctcttctgcg
     8161 gcctccatcc tctgaaaacg caacagcatc gtgcgacgcc agctgaaaaa atccgtatcc
     8221 tgcatagtaa accgcaacgc ccctgagagt gagtatcatt tataatgata attcaaacta
     8281 tcacataaat aagatttata tataatttat attattcagg caatgaatta cttttgcaag
     8341 ccatcgcatt ctcttatgtt attaatgagt tatgctgatt tgttaagcag ttttatcacg
     8401 cttgaaatgg cgtcccgccc cgacaggtga atcgtcgggg ctgatttttt cttattattg
     8461 cagcagaaac ttctcgagga actggcgggt gcgaggctgc tggggatcag caaataacgc
     8521 ttttgctgcc ccctgctcga ctatccgccc ctggtccata aaaatcgccc ggtccgcaac
     8581 atcccgggca aagctcattt cgtgcgtcac aatcaccatc gtgcgctttt cctgcgccag
     8641 ctggcggatt gtgttcagga cttcgcccac cagctctgga tccagcgctg acgttggctc
     8701 gtcaaacaaa atcacctcag gacgcattgc cagcgcacgc gcaatcgcta cacgctgctg
     8761 ttgaccgcca gacaaacgac gtggatagct ggtttcttta ccggccagcc caactttcgc
     8821 cagcagctcg cgagcgcgcg ccgtggcctc ttctttcggt tcgcctttga cgatcaccgg
     8881 cccttcaata atgttttcca gcaccgtacg atgcggaaac aaattaaagt tctggaagac
     8941 aaacccgacg tgctgacgca actggcggat cagcgatttt tgctgactta atgaacgtgc
     9001 agtatcaata gtgatatcgc caacggtgat cgttcccgct tccggttgtt ccagcagatt
     9061 tatgctgcgt agcaacgtgg ttttgccgga accactcgga ccgataattg ccaccacttc
     9121 gccaggcttt acctcgaggt cgataccgtg cagcaccgtc tgaccgtgaa attttttcac
     9181 caggttctta acttcaatgg cactcatttt ggttctctct cctggcgatt aagttgattc
     9241 tcaaaatggt tctgcaacgt cgataatact gtcgccatga tccagtagat gagcgaagcc
     9301 gccaggtaca tggtgaacac ctccagcgtg cgcgaggtaa tcaactgcgc ctgacggaac
     9361 agctccggca cctggattgt cgcggccagc gacgtatcct ttaccaggct gataaagctg
     9421 ttcgacagcg gcggcaacgc cacccgcgcc gcctgtggca aaatcgcgcg acgcatagtc
     9481 tgccatggcg tcataccaat actggcagct gcttcccact gacctttatc aatagaagaa
     9541 atggcggcgc gcagtgtttc ggcggcatag gcggcagtgt tcagcgacag accaatcatt
     9601 gctgacggaa tgggatctaa ttcgatacca aactgcggca ggccgtaata gatcataaac
     9661 agttgtgcga tgagcggtgt accacgaaaa atagagatat aaaaacgcgc cagccagcgc
     9721 acgggccaga ttggcgacag gcgcatcagc gcgagaataa acccgagcag taagccaaaa
     9781 aacatgccgc cgatactgag ttgcagcgta tacccagccc ctttcagcag gaacggcaag
     9841 gaatcaataa ccagttgtat actttcttgc atgagcgttc ccggagctaa acgtggggat
     9901 gataggcgaa cagcgcaggc gcaccgccgg tatgaataaa cagaatcggc ccttcatctt
     9961 tgaagcgttt ctgactgata cagccaatca gccccgccat cgcttttccg gtatacacag
    10021 gatcaagcag aatgccttcc agccgcgcca gcaatttcac tgcttccatg ccttcgtcgt
    10081 tcggcacgcc gtagccaggt gcaaaatagt catcccagag tataatttcc gctgatgcgg
    10141 tcagctccag ttctttcgca atcgcctgtt gtaggttaac cactttcggc aactgatcgg
    10201 caacggaacg cgacacggtc acgccaatca gttcgctttc aggcatcagg tgttccagcc
    10261 caacagccag tccggcgtga gttccggcac tgcccgatgc gactaccacc gacgaaatat
    10321 taaccgcccc ttcacactgt tgcgcgattt ccagcgcact ctccacataa cctagcgcgc
    10381 caagagcatt agaaccgcca accggaatga catacgggcg aaagccttgt gcttcgactc
    10441 gcgtcgccag ctcttccagt tgggcattgg gatcggtcag tgcttcgcac atttcaatct
    10501 gggtattgaa cagatccagc aacaagcgat tgccgttggt taaatagttt tctgcggttg
    10561 tgccaatagg attttccagc agcgccacac agtgcagacc gagtttcgcc gcaactgcgg
    10621 cagtctgccg cacatggtta gactggatca ccccggcagt aatcagcgta tcggcacctt
    10681 cgcgcagagc atctgccgcg agaaattcta gcttacgtaa tttattgccg cccattgcca
    10741 tgggggtgac gtcatcccgt ttgatgaaaa tttcccgtcc tagataatca gaaaagcgcg
    10801 gcagatattc gagcggcgtt ggcgcgccga taaactccaa ccgtggaaaa cgggttaaat
    10861 tatgcagtgg cataacagcc tccgatgtat gttgttgtga ttttcttatt atgcacgctg
    10921 aaaacgcgta aataaaaaag g
//
LOCUS       DAKISA010000134        10805 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.134, whole genome shotgun sequence.
ACCESSION   DAKISA010000134 DAKISA010000000
VERSION     DAKISA010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10805
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.134"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..663
                     /locus_tag="OVW45_003735"
     CDS             <1..663
                     /locus_tag="OVW45_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCV5528083.1"
                     /translation="TKEVMRTHFPEYEIISSASAEDLTLLQLRRSGLVIADLAGESED
                     PRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLMCPTATLLSDVEPIENLVKTVR
                     SGNTHAERISAMLTSPAMNETHDFSYRSVILTLSERKVLRLLGKGWGINQIASLLKKS
                     NKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELNLPSVYGDAAEWNTAELRREMS
                     HS"
     gene            621..1298
                     /gene="bglJ"
                     /locus_tag="OVW45_003736"
     CDS             621..1298
                     /gene="bglJ"
                     /locus_tag="OVW45_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCV5528084.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1336..2124)
                     /gene="fhuF"
                     /locus_tag="OVW45_003737"
     CDS             complement(1336..2124)
                     /gene="fhuF"
                     /locus_tag="OVW45_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCV5528085.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQLYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2265..2501
                     /locus_tag="OVW45_003738"
     CDS             2265..2501
                     /locus_tag="OVW45_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCV5528086.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            complement(2540..2626)
                     /locus_tag="OVW45_003739"
     tRNA            complement(2540..2626)
                     /locus_tag="OVW45_003739"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2590..2592),aa:Leu,seq:cag)
     gene            complement(2661..2747)
                     /locus_tag="OVW45_003740"
     tRNA            complement(2661..2747)
                     /locus_tag="OVW45_003740"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2711..2713),aa:Leu,seq:cag)
     gene            complement(2776..2862)
                     /locus_tag="OVW45_003741"
     tRNA            complement(2776..2862)
                     /locus_tag="OVW45_003741"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2826..2828),aa:Leu,seq:cag)
     gene            complement(3130..4161)
                     /gene="rsmC"
                     /locus_tag="OVW45_003742"
     CDS             complement(3130..4161)
                     /gene="rsmC"
                     /locus_tag="OVW45_003742"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCV5528087.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            4264..4677
                     /gene="holD"
                     /locus_tag="OVW45_003743"
     CDS             4264..4677
                     /gene="holD"
                     /locus_tag="OVW45_003743"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCV5528088.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNGWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            4646..5092
                     /gene="rimI"
                     /locus_tag="OVW45_003744"
     CDS             4646..5092
                     /gene="rimI"
                     /locus_tag="OVW45_003744"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCV5528089.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            5107..5784
                     /gene="yjjG"
                     /locus_tag="OVW45_003745"
     CDS             5107..5784
                     /gene="yjjG"
                     /locus_tag="OVW45_003745"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCV5528090.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            5875..7464
                     /gene="prfC"
                     /locus_tag="OVW45_003746"
     CDS             5875..7464
                     /gene="prfC"
                     /locus_tag="OVW45_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCV5528091.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYSVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            7857..8462
                     /gene="osmY"
                     /locus_tag="OVW45_003747"
     CDS             7857..8462
                     /gene="osmY"
                     /locus_tag="OVW45_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCV5528092.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            8589..8750
                     /locus_tag="OVW45_003748"
     CDS             8589..8750
                     /locus_tag="OVW45_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCV5528093.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            8872..9945
                     /locus_tag="OVW45_003749"
     CDS             8872..9945
                     /locus_tag="OVW45_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCV5528094.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKVPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            9942..10724
                     /locus_tag="OVW45_003750"
     CDS             9942..10724
                     /locus_tag="OVW45_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCV5528095.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRIHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
BASE COUNT         2628 a         2708 c         2883 g         2586 t
ORIGIN      
        1 accaaagagg tcatgaggac tcacttccct gaatatgaaa taatatccag cgcctctgcg
       61 gaggacctta cattattaca attacgtcgt tccggattag tcattgctga tttagccggt
      121 gaaagtgaag atccacgttc tgtttgtgaa cattattatt ctttaatctc acaatatcgg
      181 gaaattcact gggttttcat ggtgtcacgc tcctggtatt cccaggcagt agaattgctc
      241 atgtgcccta cggcgacgtt attgtctgat gttgaaccca ttgagaatct ggtcaagacc
      301 gtacgttccg gcaatacgca cgcagagcgt atcagcgcca tgctgacctc tccggcaatg
      361 aatgaaactc atgattttag ctatcgctcc gtcattctca ctctttcaga gcgcaaggta
      421 ctgcggctat taggtaaagg atggggcatc aaccagatag cttcgttgct taagaaaagt
      481 aataaaacta tcagcgccca aaaaaacagt gcgatgcgtc gactggcaat tcacagcaac
      541 gctgaaatgt atgcatggat aaatagcgcg cagggtgcaa gagaacttaa cttgccttct
      601 gtttatggag atgccgcaga atggaacaca gccgaattaa gaagagaaat gtcgcactca
      661 tagaaaaatg cgtcatgagc agtatcggta ttgagagttt attcagaaag tttgcgggta
      721 acccttataa gctccatacc tataccagtc aggagtcatt tcaggatgcc atgtcgcgga
      781 tctcatttgc ggcggtcatt ttttcttttt ctgccatgag aagtgagcgc agagagggat
      841 tatcttgcct gactgaactg gcgattaagt ttccgcgtac ccggcgttta gttattgcgg
      901 atgatgatat tgaagctcgg ctgattggtt cattgtcgcc atcaccgctg gacggtgtat
      961 taagtaaagc gtcaacgctg gagatttttc atcaggaact ctttttgtca ttaaatggtg
     1021 tacgtcaggc gaccgaccga ctgaacaatc agtggtacat taaccaaagc cggacgctaa
     1081 gcccgacgga gagagaaata ttgcgcttta tgtcgcgtgg ctactcaatg acacaaattg
     1141 ccgagcagct taaacgcaat atcaaaacga tccgtgcaca taaatttaat gtgatgtcga
     1201 aactgggcgt cagttctgat gcagggttgt tggaggccgc agatattctg ttatgtatgc
     1261 ggcattgcga agcaagtaat gtgttgcatc cctattaatc cgcatgatgc cgggtttact
     1321 tccccggcag tgctttcatt tcagcgtaca atcgccacat tgctgcacat ccggtaagcg
     1381 ataacgctgg cagcaggttc ggcgcaccag caggccatcg cgcagcacga cggtacgcca
     1441 gagtggatta tcttcaccgt tcgtgagcgt tttctcaaaa aagagggcat ggcgcagcga
     1501 ttcaaccgtc gcctcgccga gcagttgctt catctcagtg agataccagt tgatcaaata
     1561 accggtatta ctccagataa gtttgccgtt gatctctccg gtcgcttcta gtgcctgcac
     1621 aaccggaacc agcgcctggc tgattaacgt ttccattcgc tgctgcggcg aatgtggtgt
     1681 tgcgttttta tcttcacaca catcgaccca gaaacaggcg acgcgtccgg tttcgtgaaa
     1741 ctcagcatgg aaatgttccg gcgacacatc taatgccttt tcctgcgtca gtagcgccag
     1801 cattaatggt ggcaccatca ggccgatata caattgtgcc catagtgaga tcagcggttt
     1861 gttctcgcgg atcatcgttg gttggttgcg atagatatga tcggaataga ccgcgagtag
     1921 agaacttagc gcattcggtg atgaccattg cgccagcgtc atggcgttaa gcggagcggg
     1981 ttcatccagg cggataaact cgagcaaatg ttcacgatgt ttggcgatcg tcgcacgtac
     2041 ggcttgtgca agcccagcat cctgcggctg gagatgcgtt cgccagatga tatcttcata
     2101 gagcggtgcg gaacgatagg ccataatcgg gatagtaatc taaatgataa tgattgctaa
     2161 tcctagcgat agatttaccc gatagcaagg gatttctctg gcttgcaaat gataaaaatt
     2221 atcatatgat attggttatc attatcaatg aaagagatga aaccatgttg caacgtacgc
     2281 tgggcagtgg ctggggagtg ttgctgccgg gattgctgat tgcagggctg atgtatgcgg
     2341 atttatcgcc ggatcagtgg cggattgtca ttctgatggg attagtattg acgccggtga
     2401 tgctgtatca caaacagtta cggcattacg ttttgctacc atcgtgcctg gcgcttattg
     2461 ctgccatcat gctgatgata atgaatttga atcagggatg aaaaatcaag gaagaaacaa
     2521 gaaaggaagt aaagataatt ggtgcgaggg gggggacttg aacccccacg tccgtaagga
     2581 cactaacacc tgaagctagc gcgtctacca attccgccac cttcgcacag tcatcttact
     2641 ttttttgata tcgcctcgtt tggtgcgagg ggggggactt gaacccccac gtccgtaaga
     2701 acactaacac ctgaagctag cgcgtctacc aattccgcca ccttcgccca gtgcgagcaa
     2761 tatcaacgtg gtttttggtg cgaggggggg gacttgaacc cccacgtccg taaggacact
     2821 aacacctgaa gctagcgcgt ctaccaattc cgccaccttc gcataccatc aattcttaaa
     2881 aagaattgct accacggagg cgcattctag tggttttcag cttttcgtca atagttaatt
     2941 atcgccggag gtgtaattgc tggaaaaatg tccatcagga aactagcgtg caggtttggt
     3001 aggcatgcgg gggcagatgc cagatgcgac gctggcgcgt cttatctggc ctacgaaggg
     3061 ctaacgtgca ggttttgtag gtcggataag gcgttcacgc cgcatccgac acggtattcg
     3121 gcgagataat taacctttct tcgcctggcg ggtcataatg gcgcgataca ccttgaaacg
     3181 cccggtttgc gcaatcactt catggaagcc aaatgtctca tccagcacgt ccgggtaagg
     3241 caggaaggcg ttcgctacaa ttcgcagctc gccgccgcta ttaagatgac gcaccgcgcc
     3301 gcgaatcagc gtttgcgccg catccaggct ggtttgcatc ccatcgtgga acggcgggtt
     3361 ggagatgatc atatcaaaac gacctttcac ctcggaaaag acgttgctgg caaagacttc
     3421 accttcaaca cagttggccg caagtgttgc gcggctggct tctaccgctg gcgcagagac
     3481 atcgcacaag gtgagacgaa ttttcggcga atggcgcgca aaggcaactg aaagcacccc
     3541 cgcgccacag ccgacatcca gcactttacc tttcgtgtgc ggagttaacg tcgagagcag
     3601 caactggcta ccgacatcca gaccgtcgcg gctaaacacg ccaggcagcg ttttgaccgt
     3661 caggccatcg acgctgtatt cgccccagaa tttatcggca tcaaataccg gctgtttttc
     3721 cagacgacca aaatagaggc cacagcgacg agcgctgtcg actttattca acggcgcata
     3781 atctgccagc atctgctcgg cgctgcgcac gccgctgcgg ttctcgccaa cgacaaaaat
     3841 atctgtcccc actggcagca gagaaagtaa attcatcaac tggaactggg cttccggttt
     3901 gttcttcggc cagtagtaaa tcagcgtatc gcaatctgcg acgtcatcca ccgtggcgac
     3961 cagactgaaa cgggcgttat cccccatctg gcggcttaat acctgccagt ggtggaattg
     4021 ctgggtatga gcacggctgg ccgcggtatc taaacgcgcg ggcaggtcat cctgtaagtc
     4081 tccggcaaac agaatacggc tttgttcgaa atcatcactg tgacgcagca agacttcact
     4141 tgccggggta aatgcagaca tggaatgctc ctcaattgat actggcggcg attatagcca
     4201 tatgttggcg cggtatcgac gaatttgcta tatttgcgcc cctgacaaca ggagcgattc
     4261 gctatgacat cccgacgaga ctggcagtta cagcaactgg gcattaccca gtggtcgctg
     4321 cgtcgccctg gcgcgttgca gggggagatt gccattgcga tcccggcaca cgtccgtctg
     4381 gtgatggtgg caaacgatct tcccgccctg accgatcctt tagtgagcga tgttctgcgc
     4441 gcattaaccg tcagccccga tcaagtgctg caactgacgc cagaaaaaat cgcgatgctg
     4501 ccgcaaggca gtcgctgcaa cggctggcgg ttgggtactg acgaaccgct atcactggaa
     4561 ggcgctcagg tggcatcacc ggcgctcacc gaattacggg caaacccaac ggcacgcgcc
     4621 gcgttatggc aacagatttg cacatatgaa cacgatttct tccctcgaaa cgactgattt
     4681 accagcggct taccacattg aacaacgcgc ccacgccttt ccgtggagtg aaaaaacctt
     4741 tgccagcaac cagggcgagc gttatctcaa ctttcagtta acgcaaaacg gcaaaatggc
     4801 ggcgtttgcg attacgcaag tggtgctgga tgaagctaca ttgttcaata ttgcggtcga
     4861 tcctgactat cagcgtcagg gattgggaag ggtgctgctg gaacatctga tcgacgaact
     4921 ggaaaaacgc ggcgtggcga cactatggct ggaagtccgt gcctcaaatg ctgccgccat
     4981 tgccctgtac gaaagtttag gctttaacga ggcgacgatt cgccgcaatt actaccccac
     5041 cacggacggt cgcgaagacg ccatcatcat ggcgttgcca atcagtatgt aagacaaggt
     5101 ggaataatga agtgggactg gattttcttt gatgccgatg aaacgctgtt tacctttgac
     5161 tcattcaccg gcctgcagcg gatgtttctt gattacagcg tcacctttac cgctgaagat
     5221 tttcaggact atcaggccgt taacaagcca ctgtgggtgg attatcaaaa cggcgcgatc
     5281 acttcattac agcttcagca cgggcgtttt gagagctggg ccgaacggct gaaagttgaa
     5341 gcaggcttgc ttaacgatgc ctttattaat gcgatggcgg aaatctgcac gccgctgccg
     5401 ggcgcggttt ctctgcttaa cgccattcgt ggcaacgcca aaatcggcat catcaccaac
     5461 ggctttagcg ccttgcagca agtgcgtctg gaacgcacgg gcctgcgtga ttacttcgat
     5521 ttgctggtga tttccgaaga agttggcgtt gccaaaccga ataagaaaat tttcgattat
     5581 gcgctggaac aggcgggcaa tcctgaccgt tcacgcgtgc tgatggttgg cgacactgcc
     5641 gagtccgata ttctcggtgg catcaacgcc gggcttgcga cctgctggct gaatgcgcac
     5701 aatcgcgagc aaccagaagg catcgcgccc acctggaccg tttcttcgtt gcacgaactg
     5761 gagcagctcc tgtgtaaaca ctgattgcct cccccccgtt gatgggtaaa atagccgcaa
     5821 tttttcgttt tcaacaagcg cggcgcgatg ccgcttactc aagaagaaag aattatgacg
     5881 ttgtctcctt atttgcaaga ggtggcgaag cgccgcactt ttgccattat ttctcacccg
     5941 gatgccggta agactaccat caccgagaag gtgctgctgt tcggacaggc cattcagacc
     6001 gccggtacag taaaaggccg tggttccaac cagcacgcta agtcggactg gatggagatg
     6061 gaaaagcagc gtgggatctc cattactacg tctgtgatgc agtttccgta tcacgattgc
     6121 ctggttaacc tgctcgacac cccggggcac gaagacttct cggaagatac ctatcgtacc
     6181 ctgacggcgg tggactgctg cctgatggtt atcgacgctg caaaaggtgt tgaagatcgt
     6241 acccgtaagc tgatggaagt tacccgtctg cgcgacacgc cgatcctcac ctttatgaac
     6301 aaacttgacc gtgatatccg cgacccgatg gaactgctcg atgaagttga gaacgagctg
     6361 aaaatcggct gcgcaccgat cacctggccg attggctgcg gcaagctgtt taaaggcgtt
     6421 taccaccttt ataaagacga aacctatctc tatcagagcg gtaaaggcca cacgattcag
     6481 gaagtccgta ttgttaaagg gctgaataac ccggatctcg acgctgcggt tggtgaagat
     6541 ctggcacagc agctacgtga cgaactggaa ctggtgaaag gcgcgtctaa cgagttcgac
     6601 aaagaattgt tccttgcggg cgaaatcact ccggtattct tcggtactgc gctgggtaac
     6661 ttcggcgttg atcatatgct ggatggcctg gtggagtggg ctcctgcgcc gatgccgcgt
     6721 cagactgata cccgtaccgt agaggcgagc gaagacaaat ttaccggctt cgtatttaaa
     6781 attcaggcca acatggaccc gaaacaccgc gaccgcgtgg cgtttatgcg cgtagtatcc
     6841 ggtaaatatg aaaaaggcat gaagctgcgc caggtgcgta ctgcgaaaga tgtggttatc
     6901 tccgacgcgc tgacctttat ggcgggcgac cgttcgcacg ttgaagaagc gtatccgggc
     6961 gatatcctcg gcctgcacaa ccacggcact attcagatcg gcgacacctt tacccagggt
     7021 gagatgatga agttcaccgg tattccgaac ttcgcaccag aactgttccg tcgtatccgc
     7081 ctgaaagatc cgctgaagca aaaacagctg ctcaaagggc tggtacagct ttccgaagag
     7141 ggcgcggtgc aggtgttccg tccgatctcc aacaacgatt tgatcgttgg tgctgttggt
     7201 gtgctgcagt ttgatgtggt ggtatcgcgc ctgaagagcg aatacagcgt tgaagcagta
     7261 tatgaatcag tcaacgttgc cactgcccgc tgggtagaat gtgcggacgc gaagaaattc
     7321 gaagagttca agcgtaagaa cgaaagccaa ctggcgcttg atggcggcga taacctcgct
     7381 tacatcgcta ccagcatggt caacctgcgc ctggcgcagg aacgttatcc cgacgttcag
     7441 ttccaccaga cccgcgagca ttaattcttg cctccagggc gcggtagccg ctgcgccctg
     7501 tcaatttccc ttccttatta gccgcttacg gaatgttctt aaaacattca cttttgctta
     7561 tgttttcgct gatatcccga gcggtttcaa aattgtgatc tatatttaac aaagtaatga
     7621 catttctgac ggcgttaaat accgttcaat gcgtagatat cagtatctaa agccgtcgat
     7681 tgtcattcta ccgatattaa taactgattc agaggctgta atggtcgtta ttcatcaccc
     7741 atcgcttttg tgatggcgac cattgacttc tgtagagggt gaagtctctc cctattcagc
     7801 aatgcaacct cgtgttgcca ggctcaaatt acgagcaaac atacaggaat aaatcgatga
     7861 ctatgacaag actgaagatt tcgaaaactc tgctggctgt aatgttgacc tctgccgtcg
     7921 cgaccggctc tgcctacgcg gaaaacaacg cacagactac caatgaaagc gcagggcaaa
     7981 aagtcgatag ctctatgaat aaagtcggta atttcatgga tgacagcgcc atcaccgcga
     8041 aagtgaaggc ggcgctggtg gatcatgaca acatcaagag caccgatatc tctgtcaaaa
     8101 ccgatcaaaa agtcgtgacc ctgagcggtt tcgttgaaag ccaggctcag gccgaagagg
     8161 cagtgaaagt ggcgaaaggc gttgaagggg tgacctctgt cagcgacaaa ctgcacgttc
     8221 gcgacgctaa agaaggctct gtgaagggct acgcgggtga caccgccacc accagtgaaa
     8281 tcaaagccaa actgctggcg gacgatatcg tcccttcccg tcatgtgaaa gttgaaacca
     8341 ccgacggcgt ggttcagctc tccggtaccg tcgattccca ggcacagagc gaccgtgctg
     8401 aaagtatcgc caaagcggta gatggtgtga aaagcgttaa aaatgatctg aaaactaagt
     8461 aattcgtcgt aattcgtcct cccgaacttt gtcgggaggc gcgatgtgca ccacactaaa
     8521 aatatcgcat tgagtgattt tcacgctcat atcaagcggt gacattaact atggtaaagg
     8581 agacgcttat gtttcgttgg ggcatcatat ttctggttat cgcgttaatc gccgccgcac
     8641 ttgggtttgg tggtctggcc ggtaccgctg caggcgcagc taaaattgtc tttgtcgtcg
     8701 ggattattct gttcctggtg agtttgttca tgggccgaaa acgaccctag atttcagacc
     8761 attaagaaaa ttttatccaa agccagtcca gcggactggc ttttgcggtt ttagcgaata
     8821 ataaattgcg ttactttgtc attctttcac aacaaaaaca ggaaggcaga ggtggggcag
     8881 cgaatacctg taacgcttgg taatattgcg ccgttgtcgc taaggccgtt ccagcctgga
     8941 cgaatagctc tggtgtgcga aggcggcgga cagcgtggaa ttttcacggc tggcgtgctg
     9001 gatgagttta tgcgcgcgca gtttaatcct ttcgatcttt atctcggcac atctgccggg
     9061 gcgcagaacc tctcggcgtt tatctgcaat cagcccggtt acgcgcgcaa agtcatcatg
     9121 cgctatacca caaaacgcga atttttcgat ccattgcgct ttgtccgtgg aggaaatctt
     9181 atcgatctcg actggctggt ggaggccacc gcaagccaga tgccgttgca aatggatacc
     9241 gccgcgcggt tgtttgacag cggcaaatcg ttttatatgt gcgcctgtcg tcaggatgac
     9301 tacgcgccta attacttttt accaaccaaa caaaactggc tggatgtgat tcgcgcctcc
     9361 agtgcgatac ctggctttta tcgtagcgga gtgtcgctgg aaggcattaa ctacctggat
     9421 ggcgggatca gtgatgcgat tccggttaaa gaggcggcaa ggcagggcgc taaaacgttg
     9481 gtcgtcattc gcactgtgcc gtcacaaatg tactacacgc cgcagtggtt caaacgcatg
     9541 gaacgctggc tgggtgacag tagcctgcag ccgctggtca atctggtgca gcatcatgaa
     9601 accagctatc gtgacattca gcaatttatt gagaaaccac cgggcaagct gcggatattc
     9661 gaaatttatc cgccgaagcc attacatagt atcgcgcttg gcagtcggat tccggcgctg
     9721 cgtgaagact ataaacttgg gcgtttatgc ggtcgttatt tcctcgccac ggttggcaag
     9781 ctattaactg aaaaagtgcc gcttacccgc catctggtgc cagtggtgac gccggaatcg
     9841 atcgttattc cgcctgcgcc agtcgccaac gatacgctgg ttgccgaagt gagcgatgct
     9901 ccgcaggcga acgacccgac atttaacaat gaggatctgg cttgatttgc cgttttatcg
     9961 atacccactg ccattttgat ttcccgccgt ttagtggcga tgaagaggcc agcctgcaac
    10021 gcgcggcaca agcgggcgta ggcaagatca ttgttccggc gacagaggcg gaaaattttg
    10081 cccgcgtgca ggcgttagcg gaaaaatatc aaccgctgta tgccgcattg gggttgcatc
    10141 cggggatgtt ggaaaaacat agcgatgtgt ctcttgatca gctacagcag gcgctggaaa
    10201 ggcgtccggc gaaggtggtg gcggtggggg agatcggtct ggatctcttc ggcgacgatc
    10261 cgcaatttga gaggcagcag tggttactcg acgaacagct gaaactggcg aaacgctacg
    10321 atctgccggt gatccttcat tcccggcgta tacacgacaa actggcgatg catcttaaac
    10381 gtcacgattt accgtgcact ggcgtggttc acggtttttc cggcagcctg caacaggccg
    10441 agcggtttgt acagctgggc tacaaaatcg gcgtaggcgg tactatcacc tatccacgcg
    10501 ccagtaaaac ccgcgatgtc atcgcaaaat taccgctggc atcgttgttg ctggaaaccg
    10561 acgcgccgga tatgccgctc aacggctttc aggggcagcc taaccgaccg gaacaggctg
    10621 tgcgggtgtt tgacgtgctt tgcgagttgc gcccggaacc agaagatgag attgccgaag
    10681 tgttgcttaa taatacgtat gcgttgttta gcgttagtgg gtagcctacc cttcgtaggc
    10741 agcatccggc agtcggcgca taatgcctga tgcgacgctt aacgcgtctt atcatgccta
    10801 caggt
//
LOCUS       DAKISA010000135        10723 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.135, whole genome shotgun sequence.
ACCESSION   DAKISA010000135 DAKISA010000000
VERSION     DAKISA010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10723
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.135"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..716
                     /locus_tag="OVW45_003751"
     CDS             <1..716
                     /locus_tag="OVW45_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528096.1"
                     /translation="LSPITSFDYIHFITTHPSGIKDTLAWLVNASKLMTEFDDNGKII
                     FNINALKYTKASYFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLELAIGWRGAL
                     SESIKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGGVATAIGSLA
                     SFACVSFGFPVILVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDGLKRQQELDK
                     WKRENMTPFMYVLNTPPVI"
     gene            726..1205
                     /locus_tag="OVW45_003752"
     CDS             726..1205
                     /locus_tag="OVW45_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528097.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAFNASLFFTGVKPLYSIIKCST
                     EIFYDWRMLILCFGFMSFSFLNIHVILLTIIKLFLIKKTKVVNFATDITIQLTLIFLL
                     IAIVIAPLIAPFVTGYVNANYHPCGNNTGIFPGAIYIKNGMKCNNEYISRKEDSAVK"
     gene            complement(1342..2127)
                     /locus_tag="OVW45_003753"
     CDS             complement(1342..2127)
                     /locus_tag="OVW45_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528098.1"
                     /translation="MNLRRVCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSGLKSIVWSDYKNQKLTDTMSLRYLVRFTLVDVATGEWAAWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
     gene            complement(2457..2533)
                     /locus_tag="OVW45_003754"
     tRNA            complement(2457..2533)
                     /locus_tag="OVW45_003754"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2497..2499),aa:Asp,seq:gtc)
     gene            complement(2666..3397)
                     /gene="dnaQ"
                     /locus_tag="OVW45_003755"
     CDS             complement(2666..3397)
                     /gene="dnaQ"
                     /locus_tag="OVW45_003755"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCV5528099.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            3462..3929
                     /gene="rnhA"
                     /locus_tag="OVW45_003756"
     CDS             3462..3929
                     /gene="rnhA"
                     /locus_tag="OVW45_003756"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCV5528100.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(3926..4648)
                     /locus_tag="OVW45_003757"
     CDS             complement(3926..4648)
                     /locus_tag="OVW45_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCV5528101.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            4682..5437
                     /gene="gloB"
                     /locus_tag="OVW45_003758"
     CDS             4682..5437
                     /gene="gloB"
                     /locus_tag="OVW45_003758"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCV5528102.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            5509..6867
                     /gene="mltD"
                     /locus_tag="OVW45_003759"
     CDS             5509..6867
                     /gene="mltD"
                     /locus_tag="OVW45_003759"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCV5528103.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(6916..7512)
                     /locus_tag="OVW45_003760"
                     /pseudo
     CDS             complement(6916..7512)
                     /locus_tag="OVW45_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(7543..8343)
                     /locus_tag="OVW45_003761"
     CDS             complement(7543..8343)
                     /locus_tag="OVW45_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCV5528104.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            8584..9498
                     /locus_tag="OVW45_003762"
     CDS             8584..9498
                     /locus_tag="OVW45_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCV5528105.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(9495..10298)
                     /gene="dkgB"
                     /locus_tag="OVW45_003763"
     CDS             complement(9495..10298)
                     /gene="dkgB"
                     /locus_tag="OVW45_003763"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCV5528106.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(10461..10537)
                     /locus_tag="OVW45_003764"
     tRNA            complement(10461..10537)
                     /locus_tag="OVW45_003764"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10501..10503),aa:Asp,seq:gtc)
BASE COUNT         2953 a         2457 c         2433 g         2880 t
ORIGIN      
        1 tactcagccc aataacatca tttgattaca ttcattttat cactacacat ccttcaggta
       61 taaaagatac actcgcctgg ttagttaacg cgagtaaatt aatgactgag tttgatgaca
      121 atgggaaaat tatatttaac attaatgcct taaagtatac aaaagcaagt tactttgaaa
      181 tacttggaga gaaatatata aaaatcacaa catccagccc ctggctttta gaaaagctcg
      241 gtaaatatat tttctcttca cgagcgcctc aagttctgga actcgctatt ggctggcgag
      301 gagctttatc tgaatcaata aaaggagtta agttctgtat ctggttttca gtagcctgga
      361 gaaccataga atttattatg agttcagagc gagatctggt taattttctt ggcgattttt
      421 caatggatgt ggccaaagca gttatagccg gtggtgttgc aaccgctatt ggaagcctgg
      481 cttcttttgc ctgtgttagc tttggctttc cagtaattct tgtcggagga gcaattttac
      541 tgacagggat agtgtgtaca gttgttttaa atgaaatcga tgctcaatgc catttatcag
      601 aaaaattaaa atatgcaatt agagatggac taaaacggca acaggaactt gataaatgga
      661 aaagggaaaa catgactcca tttatgtatg ttcttaacac tccacccgtg atatgaggat
      721 taaccatgcg tttacatgtt aaattaaaag agtttctttc gatgtttttt atggccatat
      781 tatttttccc tgcattcaat gcttctttgt tttttaccgg agttaaacct ctatattcaa
      841 taataaaatg ctccacggaa atattttatg attggagaat gttgattctg tgctttggtt
      901 tcatgtcgtt ttccttttta aatattcatg taattttact tacgataata aaattattct
      961 taatcaaaaa aacgaaggta gtcaatttcg ccacagacat aacaatacag ctaactctta
     1021 tatttttact aatagctatc gtaatagcac ctttaatagc tccttttgtc acaggatatg
     1081 tcaatgctaa ttatcacccc tgcggtaata atacgggaat tttcccagga gcaatctata
     1141 ttaaaaatgg catgaaatgt aataatgaat atatttctcg caaggaagat tcagcagtta
     1201 aataattaaa cattctaaaa caaacagttt ttaactccgg attccatgtc ctttcttgca
     1261 ctgtctttgt caagaaaaat ttcttatgca aagaattttg tattatttcc gccagaaatt
     1321 cctggcggca gatataattt attattgata gcggttaacc aaatctttta ccatcgcttt
     1381 ataggtcttt tgcttaagtt gcatgatttg ctgctctgtc atatcagtcg tactggcttc
     1441 attcttgtcg ggcaatggtg gaagcacttt atattcataa ttcaccggcg accaggcagc
     1501 ccactcacct gtagctacat ccaccagcgt gaatcgtacc agatagcgca gtgacatcgt
     1561 gtcagtcaat ttttgatttt tataatctga ccaaactatt gatttcaatc cagagtcata
     1621 ttttcctgtc tgcaacgtat cctggaaaac aataatcgct ttctgatgcc cttttgcagc
     1681 cacaaaacgc agtgcctgca tccagttcat attctcagca ctggcaacat cctcgttctc
     1741 atttttatct ttgtttttgt tacaagtcag aggcttttgc ctgtccggga taccagagaa
     1801 tgtggaaaca gtataatatt ttcgcatctc ctcctgcata atggtttccg gtgcgcggtt
     1861 gccggattga acaagtatta caggagaatt aaggggaacg cggaactcat ctccatcaag
     1921 cgcagcttga atatcctctt cggatacggc cagagtggtt tcattaccga ataaatcttt
     1981 gtctgtcagt tgctggggag tgtttttatt aatattactt tgtgtgactt gagctttttt
     2041 cttcttttca tcaatagaag attcagaggc acatcctgca agcagcgaga ataatacaca
     2101 tatacaacaa acccttctta aattcataat cggacactcc ctcgcctgtc ataatcatgg
     2161 taatgtttag ctaacgattt ttaattatat actccacttg aatcagaaca aacaaaaata
     2221 acagtatata taaattcatt aaatcaatag aaaaatgaaa atcataacat tcaaatatat
     2281 ttaaattgaa tgattcgata gtatgattaa atattaatga tatggtctac catatgtgat
     2341 taacacattt acagcataac aaaaccatag accgaagaaa tagcgggatt taaatgcaaa
     2401 aaaaccgcaa aattaaaaat cttgcggttc tctgaactca ttttcatgag tgaatttggc
     2461 ggaacggacg ggactcgaac ccgcgccccc ctgcgtgaca ggcaggtatt ctaaccgact
     2521 gaactaccgc tccgcgttgt gttccgttgg gaacgaggcg aatattacgg attgcctcac
     2581 ccttcgtcaa cggtttttct catcttttga atcgtttgct gcaaaaatcg cccaagccgc
     2641 tatttttagc gccttttaca ggtatttatg cccgccagag gcagcttccg cccttcttct
     2701 ccaccagatc aagacgggct tcatgagctg caagctcttc atctgtcgca aaaacaacgc
     2761 gtaacttact tgcctgacgt acaatgcgct gaattgttgc ttcaccttgt tgctgctgtg
     2821 tctctccttc catcgcaaaa gccatcgacg tttgaccacc ggtcatcgcc agataaactt
     2881 ccgcaaggat ctgtgcatcg agtaatgccc cgtgtagcgt tcgtttactg ttatctattt
     2941 cgtagcgggc acataacgca tcgaggctgt tgcgcttacc aggaaacatt ttcctcgcca
     3001 ccgcaaggct atcggtgacc ttacagaagg tattggtctt cggaatatcg cgcttaagca
     3061 acgaaaactc gtagtccata aagccgatat cgaacgctgc gttatggatc accaactccg
     3121 cgccgcggat atagtccatg aactcatcgg ctacttcggc aaacgtgggc ttatcgagca
     3181 aaaattcatc ggcaatacca tgtacgccaa aggcttccgg atccaccagc cgatcgggtt
     3241 tgagataaac atggaagtta ttgcccgtca ggcgacggtt caccacttca acggcaccaa
     3301 tctcaatgat cttgtggcct tcatagtgcg caccaatctg gttcataccg gtggtttcgg
     3361 tatcgagaac gatctggcgt gtaattgcag tgctcatagc ggtcatttat gtcagactta
     3421 tcgttttaca gttcgattca attacaggaa gtctaccaga gatgcttaaa caggtagaaa
     3481 ttttcaccga tggttcgtgt ctgggcaatc caggacctgg gggttacggc gctattttac
     3541 gctatcgcgg acgcgagaaa acctttagcg caggctacac ccgcaccacc aacaaccgta
     3601 tggagttgat ggccgctatt gtcgcgctgg aggcgttaaa agaacattgc gaagtcattt
     3661 tgagtaccga cagccagtat gtccgccagg gtatcaccca gtggatccat aactggaaaa
     3721 aacgtggctg gaaaaccgca gacaaaaaac cagtaaaaaa tgtcgatctc tggcaacgtc
     3781 ttgatgctgc cctagggcaa catcaaatca aatgggaatg ggttaaaggc catgccggac
     3841 acccggaaaa cgaacgctgt gatgaactgg ctcgtgctgc ggcaatgaat cccacactgg
     3901 aagatacggg ctaccaagtc gaagtttaag cttgtggttt acgacattgc cgggtggctc
     3961 caaccgcctg gcgaattcgt ggcttgtttt tactctgttt catcgggttt agcgttaaag
     4021 gaatagtccg tttccgggca acaataagtt gtaagcaacc aagcgcagga atatgcgcat
     4081 tcaatagttt tcctccgtgt ttgttccacg ggagaacgtg gaaacggcta gcgtgcagca
     4141 cttcaaaatt caacaaagag agccagtcca gttgccgcat cagagtaaac atccggctgt
     4201 tatagggcga ggtttttcgc aataccggca caagtttgcg taatcccatt aaactaatgg
     4261 gattgaagcc actaatcacc agccagccat catcaatcaa tacccgatcg gtttcacgca
     4321 ataaacgatg cggatcggtg caccacggca atgtatgtgc cagtagacaa acatcaacgg
     4381 atttatcggc aaaaggaaga tgaagtggat ccgcctgcac ctggacgggc attccttgcg
     4441 cagaaacatt cacttgatga gaaaccgcgc acgcttcgca attgatttct gcgcttaaat
     4501 tgccaatctt aagcagatga aaaccataca ttttagtgaa ccacgggttg agctggcgct
     4561 ccagcgcctt gcgataaagc tctccccagg gcaaatcgcc ccagcgatca ggagccacga
     4621 cagtttgagg gactcttgcc ggtttcatca caaccttccg tttcacacta agaggtaatc
     4681 tatgaatctt aacagtattc ccgcctttga tgacaattac atctgggttt tgaatgatga
     4741 agcaggtcgc tgcctgattg tcgatcccgg agacgcagag ccagtattaa acgccattgc
     4801 cgccaataac tggcaaccgg aggccatatt tctcacccac catcatcacg atcacgttgg
     4861 cggcgtaaaa gaactggtgg aaaagtttcc gcaaattgtg gtgtatggtc cacaagagac
     4921 acaagataag ggaacaacac aggtagtcaa agatggcgaa actgccttcg ttttggggca
     4981 tgaatttagt gtaattgcca caccgggtca cactttagga catatctgtt acttcagtaa
     5041 accttatcta ttttgcggcg acacgctgtt ttctggtggg tgtggtaggt tgtttgaagg
     5101 gacagcatca caaatgtatc aatcacttaa taagttaagt gcgttacctg acgatacatt
     5161 ggtatgttgt gctcatgaat atacgttatc aaatatgaag tttgctttga gtattcttcc
     5221 gcacgatttg tccataaatg attattatcg taaagttaag gagttacggg caaaaaatca
     5281 aataacacta cccgtaattc tgaaaaatga gcggcaaatt aatgtttttt taagaacgga
     5341 agatattgat ttaattaatg taattaatga agaaacatta ttgcaacaac ctgaagagcg
     5401 ttttgcatgg ttaaggtcaa agaaagatag gttctgataa aactttcttg tcatcggctc
     5461 cgttcgccgt tatgatcggt cgtcttttaa gcaactattg acacacacat gaaggcaaaa
     5521 gcgatattac tcgcctctgt cctgctcgtg ggttgccaga gtaccggcaa cgttcaacag
     5581 cacgcacaga gcctttctgc agctggtcaa ggggaagcag caaagtttac aagtcaggca
     5641 cgatggatgg acgatgggac gtctatcgcg ccagatggtg acttgtgggc tttcattggc
     5701 gacgagctaa agatgggaat tccggaaaat gaccggattc gcgaacagaa acagaaatat
     5761 ttacgcaata agagctatct ccacgatgta actttacggg cagagccgta tatgtactgg
     5821 atagcagggc aagttaaaaa acgtaacatg cctatggaac tggtactact acccatagtg
     5881 gagagcgctt ttgatcctca cgcaacgtct ggcgccaatg ccgcaggcat ctggcagatc
     5941 attccgagca cggggcgcaa ttatggtttg aaacagaccc gcaattatga cgcgcgtcgc
     6001 gatgttgttg cttcaacaac tgccgcgctg aatatgatgc agcgtctgaa caaaatgttt
     6061 gacggcgact ggcttctgac cgtagcggct tataacagcg gcgaaggtcg ggtcatgaag
     6121 gcaattaaaa cgaacaaagc gcgtgggaaa tccacggact tctggtcgtt accgttgccg
     6181 caggaaacga agcagtacgt gcctaaaatg ctggcattga gtgatattct caaaaacagc
     6241 aagcgttatg gcgtacgtct gccaacgacc gatgaaagcc gtgctctggc gcgtgtgcac
     6301 ctgagtagcc cggttgaaat ggcgaaggtt gcagatatgg cggggatttc cgtcagcaag
     6361 ctgaagacat tcaacgctgg cgtgaaaggc tccacgctgg gcgcaagtgg gccgcagtac
     6421 gtgatggtgc caaagaagca tgcagatcaa ctgcgtgaat ctctcgcttc aggcgaaatt
     6481 gctgctgtac agtcgacgct ggttgccgac aatacgccgc ttaacagccg tgtttacacc
     6541 gtacgctctg gcgacacgct ttcaagcatc gcttcacgtc tcggcgtaag caccaaagat
     6601 ttgcagcagt ggaacaaact gcgcggatct aagctgaagc caggccaaag tttgacgatt
     6661 ggtgcaggca gtagcgcaca gcggttggca aacaacagcg atagcattac gtatcgtgtg
     6721 cgcaaaggcg attcgctttc aagcattgct aaacgccacg gcgtgaacat caaagatgtg
     6781 atgcgctgga acagcgacac tgcgaatctg caaccaggcg ataagctgac gttgtttgtg
     6841 aaaaacaaca acatgccaga ttcctgacaa accagataat aaaaaaggca ccgattcccc
     6901 cggtgccttt tttatttatg ccgctttatg tgcttctacc atgatgatat cactggtgaa
     6961 agagccatca gtctgcaagg aaaaatacgt tctcacctct gtcgatgcgc tctgttggta
     7021 aatgcgaata gcgtctacta acgcttctgg cgtacgcatt ctcacgaccc atgaagaaaa
     7081 ttccagcggt aacttatctg taattaaatt atcaactatc agattggctt cattgattaa
     7141 cgtcaaccac gcaccgctgg cgtagtttcg tacgtgagag gtatcgcgta atgcttctac
     7201 cgcctgtaac cagatgtcgc gcactgggtg acccggagac attacatcca tcacaatcag
     7261 cctaccgcca ggtttcaata tcctattcac ttctcgcagt gctgcaccaa catcatgcca
     7321 gtgatgggca gaataacggc tgataacaat atcaaatgcg ttatcggcaa atggcagact
     7381 tgcggcatat ccctggcggg tggtgatatt tttcagttgc cgggcttcgg cagcttgtgc
     7441 cacgacatcc agcatgtggg cagataagtc atacgccacc accgcgctca cgttttgcgc
     7501 agcgccaaac gttggtctgc cctgcggcag acctgacata ccttatttat caggcttgcc
     7561 gggactgaat tcaacgagta gcggattgtg atcggaagcg cgcgtaacca gtacagaagc
     7621 ttcactgacg ttcagaccac ggtagaaaac aaaatcgagc gggcgaccaa acgcccgacg
     7681 gcgctgatca tcggtaaaac gcacctggcg cagcgacatt tcccgcgcaa agcgatataa
     7741 cgcgttcatc cttctacggc tccaggcatt gaaatctccc gccataatga ccgggccgct
     7801 gtggtgagct atctgatcac caataggaag taactgctta ctatagacat ccacgcccag
     7861 actgaagttg acggcgtgta tattaaccac catcaacagg cgggtgtcag gtaatggata
     7921 gaccgtcacc agtgccgact tcgccagacg caaaatgggt tctcgttcac gtaacgggca
     7981 gcaatacact ggatgtgccg ccgaaagggt cattacgcca gaaggatgtt gtggcagcac
     8041 gaaagcgggt acctgatcgg cggcaagata gttagcggtc gcaaactgta ccaactctgg
     8101 cgttgtttgc gcttcctgca ataacaccag atgtgcgtct ttgccgtaat tctttaatac
     8161 cgacaaccat tcagcgcgtt gctgtttgta tatgttccac accaggatcc gaatacgctc
     8221 ttcggtactt aacggttctc cgggtggtaa tgcctggccg atgctcgcaa aagaccccgg
     8281 cggtaagatc ctctccgcag gttgtccggc aacatagcgc atggcatagg tgttttttcg
     8341 cacttttgac tttgaaacct ctattacgtc aacccttccg cactcgggag ggttctactg
     8401 ttatagggat tttagctcac tctttcaacg agcaattgta tattgttatg taagcaagtg
     8461 cttacaccgg tcagaaattt gctcattatt gagaacatta aatcggatga catatcacgc
     8521 atcctgatgg tttaatgaca aataaattca aaatatctca tctattttgc aacaggagca
     8581 aaaatgaaag ccacgtcgga agaactcgcc atttttgttt cggtcgtcga aagcggcagc
     8641 tttagccggg cagcggaaca attagggcaa gcaaactcag cggtaagccg ggcggtgaaa
     8701 aagctggaaa tgaaacttgg cgttagcctg cttaatcgga ccacgcggca acttagcctg
     8761 acggaagaag gcgagcgtta ttttcgtcgc gtacagtcaa ttttgcagga gatggcagcg
     8821 gcagaatcag aaattatgga gacgcgtaat acaccgcgtg gactgttacg gatcgatgcc
     8881 gcaactccag tggtgctgca ctttctgatg ccgttaatta agcctttccg tgaacgctat
     8941 ccggaagtca ctttatcgct agtctcctcc gaaacgatta ttaatttgat cgaaagaaaa
     9001 gtggatgtcg cgatacgcgc tggtacgtta acggactcca gcttacgtgc caggccgtta
     9061 tttaacagtt atcgaaaaat tatcgcctcc cccgattata tttcccgcta cgggaagcca
     9121 gaaacgatcg acgatttaaa gcaacatgtt tgcctgggat tcactgaacc cgcttccctc
     9181 aatacctggc cgatagcctg tagtgatgga caattacatg aggtgaagta cggtttgtca
     9241 tccaatagtg gggaaacact gaaacagctt tgcctgagtg gcaacgggat tgcgtgtttg
     9301 tccgactaca tgatcgacaa agaaatcgct cgcggagaat tggtggagtt aatggcagat
     9361 aaagtgttgc cagtggaaat gccattcagt gccgtctatt acagcgaccg tgcggtaagt
     9421 acgcgcatcc gggcttttat cgatttcctt agcgagcatg taaaaacagc tcccggagga
     9481 gctgtcagag aggcttaatc ccattcagga gccagacctt ccgggctaac caggcggtcg
     9541 ttgcaatcca gtgcggcgat cgctttttta tcttcggcat caagctgtaa attttgtgcc
     9601 ttaagattac tttccaggtt tttacgttta gtagaagaag gaattactga gtaaccttcc
     9661 cccatagccc acgccagaat cacttgtgcc ggagtcgcat tgtgtttagc tgcaatacga
     9721 gcaataacct catctttcag tgccttacca taagccagcg tcatatagga agtgatatga
     9781 ataccgtgct gtttagccca ggcaaccact ttacggtttt gcagataagg agagagttca
     9841 atctggttgg tagcgatgtt ttcagcgcca acagcagcaa tcgctttttc catcaacggg
     9901 atcgtgaagt tggaaatacc gatctcacgt gtcagacctt gttttttggc ctccagcagc
     9961 gcctgcataa actcttcaac agagacttca tcgtttggtg acggccagtg gattagcgtc
    10021 agatcaacat aatcggtacg caatttttgc aggctctctt ttagactcgg gatcaatttg
    10081 tctttgctga gattttcaat ccagatttta gtggtgatgt agagttcatg acgtggcacg
    10141 ccactttctg caatcgcctg acctactgcg gcttcgttat catagatttg tgcagtatca
    10201 attgcgcgat aaccaagttc aagcgccgtt ttcacagatg aaataacaac gtcgtctttg
    10261 agacggaaag tacctaaacc aaatgcaggg atagccataa tatgcctctt ttaatgctat
    10321 tttcgttaac tgaggtgatt atgggcttca gactgttgcg gaaaaagagc ataaaatgca
    10381 gaggattttt gcgattctgg caataataga tatacagacg aaaaaaaacc tcgagcgtta
    10441 actcgaggct tcttaataag tggcggaacg gacgggactc gaacccgcga ccccctgcgt
    10501 gacaggcagg tattctgacc gactgaacta ccgctccacc gaattctttt acaaccaccg
    10561 gttttatgac cggcttactg cttaatttga tgcctggcag tttatgaatc acttcgtgat
    10621 tcacccttcg ggccgttgct tcgcaacgtt caaaagcttt cgcttttgtc ctactctctt
    10681 atcgagtagt gaactgcctc aaaggtcagt gccattcttc gta
//
LOCUS       DAKISA010000136        10341 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.136, whole genome shotgun sequence.
ACCESSION   DAKISA010000136 DAKISA010000000
VERSION     DAKISA010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10341
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.136"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(42..1046)
                     /gene="cra"
                     /locus_tag="OVW45_003765"
     CDS             complement(42..1046)
                     /gene="cra"
                     /locus_tag="OVW45_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCV5528107.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1128..1214)
                     /locus_tag="OVW45_003766"
     CDS             complement(1128..1214)
                     /locus_tag="OVW45_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCV5528108.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1226..1717)
                     /gene="ilvN"
                     /locus_tag="OVW45_003767"
     CDS             complement(1226..1717)
                     /gene="ilvN"
                     /locus_tag="OVW45_003767"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCV5528109.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVASTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1720..3444)
                     /gene="ilvI"
                     /locus_tag="OVW45_003768"
     CDS             complement(1720..3444)
                     /gene="ilvI"
                     /locus_tag="OVW45_003768"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCV5528110.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVHNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3563..3607
                     /locus_tag="OVW45_003769"
     CDS             3563..3607
                     /locus_tag="OVW45_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528111.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3762..4706)
                     /gene="leuO"
                     /locus_tag="OVW45_003770"
     CDS             complement(3762..4706)
                     /gene="leuO"
                     /locus_tag="OVW45_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCV5528112.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5365..5451
                     /gene="leuL"
                     /locus_tag="OVW45_003771"
     CDS             5365..5451
                     /gene="leuL"
                     /locus_tag="OVW45_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCV5528113.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5544..7115
                     /gene="leuA"
                     /locus_tag="OVW45_003772"
     CDS             5544..7115
                     /gene="leuA"
                     /locus_tag="OVW45_003772"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCV5528114.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVETAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSSAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            7115..8206
                     /gene="leuB"
                     /locus_tag="OVW45_003773"
     CDS             7115..8206
                     /gene="leuB"
                     /locus_tag="OVW45_003773"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCV5528115.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKRF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8209..9609
                     /gene="leuC"
                     /locus_tag="OVW45_003774"
     CDS             8209..9609
                     /gene="leuC"
                     /locus_tag="OVW45_003774"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCV5528116.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVKRASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9620..10225
                     /gene="leuD"
                     /locus_tag="OVW45_003775"
     CDS             9620..10225
                     /gene="leuD"
                     /locus_tag="OVW45_003775"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCV5528117.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
BASE COUNT         2709 a         2717 c         2526 g         2389 t
ORIGIN      
        1 gccagaggga aattcacctg gcgcgtattt ttgttcgcgg cttagctacg gctgagcaca
       61 ccgcggcgat agagattacg tttaatgcgc gttaaaccag gttttggctt acgcggttcg
      121 tccaggcttg ccaggacaat ctccagcaca cgctctgcga catcgcggtg acgttgagcc
      181 actgccagca ccgggcactg taagaagtcg agcagttcgt tatcgccaaa ggtggcaatt
      241 gccaggtcag aaggcagttt accatcgcga cgtagcgtga catccatcac cccttgcaac
      301 aacgcaaacg acgttgtgaa cagcgcctgc ggcatcggat gcgtttccag ccatttttcg
      361 aataactggg cagccgcctc ccgctcatag ctgttggcat acaggaaatg cacttcgcgc
      421 ggatcatctt tccaggcagt acggaaacct tgttcacgca ggaagctgac agaaagctcc
      481 ggcagcgcgc caagataaag caccgtctcg gcgggaaact tacgtaactc ttccgccagc
      541 atttcggcat catcctgatc ggcaccaacc acgctggtga agtgttcacg atcgagggcg
      601 cggtccagcg cgacaatcgg gaacgggtcg ttagcccagc gttgataaaa aggatgctca
      661 ggaggcaacg acgtcgaaac aataatggca tcgacctgac gctgtaaaag gtgctcaata
      721 cagcgcattt cgttgtctgg ctgatcttct gagcaggcaa ttagcagttg ataaccccgt
      781 tgccgcgcct ggcgttcaag atagttagcg atgcgggtat agctggtgtt ctccagatcg
      841 gggatcacaa gaccaataga acgtgtgcgt ccagcacgaa gcccagctgc cacggcgttc
      901 gggtggtaat tgtgctcacg caccacagcc atgacttttt caacggtttt gtcgctcaca
      961 cggtattgct tcgctttgcc gttaataaca tagcttgcag tggtccgcga cactcccgcc
     1021 agccgagcga tttcatccag tttcacaatt gccccttgcg taaaatgtaa aaaccatagc
     1081 cattgtactg gtatgggtta aattcattaa catctaagcg cataaaatta actgcggcaa
     1141 ctgcttttat tccaggttcc cactgatttc gcaaaaaaag cccaacgtga catgttgggc
     1201 tgcaaattgc gcattcagat cattctcaac gcattatttt atcgccgcgc gaaagtccga
     1261 ccacaccaga acgagcaact tccacaattt tcgccacctc gcgaatcgat gctaaaaatg
     1321 catcaagctt atcgctggtg cctgctaatt gaacggtata aagcgagggt gtgacatcga
     1381 taatttgccc acggaatatt tccgtattac gtttcacttc gtcacgcccg taaccgctgg
     1441 cctgaatttt caccagcatg atttcccgct caacatgcgc gccctgcccc aactcactca
     1501 cgcgcaagac atcgaccagc ttgtgtaatt gcttttcgat ctgctcaagt actttttcat
     1561 cgcccacggt ctggatggtc atacgcgata atgtcggatc atcggttgac gcaacggtca
     1621 ggctttcaat gttgtagcca cgctgggaaa aaaggccaat cacgcgggat aacgcgcctg
     1681 attcattttc gagtaagact gataatatcc ggcgcataat caggttctct ccgttttgct
     1741 taaccacatt tcatccattc cgcccccgcg aatctgcatc gggtagacgt gctcgctgcc
     1801 atcgacggta acatcaacaa acaccaggcg attattgtgc acctgttcca gcgcctcgct
     1861 aagtttgctt tccagctcat gcggatgaga aatctggatc ccgacgtgac cataggcttc
     1921 cgccagacgg acgaaatcgg gtagcgattg catataagat tgtgaatgac ggccggaata
     1981 gatcatgtcc tgccactgct tcaccatccc cagatagcgg ttattgagat tcaccaccag
     2041 tacgggcaac tcgtattgca acgcggtaga cagttcctgg atgttcatct gaatactgcc
     2101 gtcgccagtg acgcaaacca cggtttcttc tggcaacgcc attttgacgc ccagtgccgc
     2161 aggtaaacca aaacccatcg tgccgaggcc accggaattg atccagcgac gcggtttgtc
     2221 gaatggataa taaagtgcag caaacatctg gtgctgcccg acatcggacg tcacgtaagc
     2281 gtctcccttc gtcaaccgcc aaagagtctc gatcaccgcc tgcggtttaa tcttttcact
     2341 gtgagtgtca tatttcaggc actgacgagc gcgccactgt tcaatttgct gccaccagtc
     2401 gcggatctca tccagtggtt gatgggcgga ttcttgcgac aagagttcaa gcatttgttc
     2461 gaggacctgg cgagcatccc ccacaatcgg gatatccgca gtcacggttt tagaaatgga
     2521 agtaggatca atatcgatat gcagaacagt ggcatttggg cagtactttg ccagattgtt
     2581 cgtcgttcgg tcatcaaatc gtaccccgac ggcgaaaatc acatccgcgt tatgcatcgt
     2641 catattggct tcgtaggtac cgtgcattcc cagcatgccc agtgcctgac gatgcgttgc
     2701 cggaaacgct cccagcccca tcaatgagga aacaacgggc agattcaacg cctccaccgt
     2761 ttctttcaac tgctgatggc agcccgccgt gattgcccca ccgcctacgt agacaaccgg
     2821 tttttttgcc gctaccagcg tttgcagagc gcgcttaatt tgccctttat gtccggtagt
     2881 agtgggattg taagaacgca tactgaccga ctccggccag acatagggta atttgttcgc
     2941 cggattaaga atatctttcg gtaaatcaac gactactggt ccaggacgac cacttgccgc
     3001 cagccagaaa gcctttttca gcacctgcgg aatgtcttcc gtttgcttaa ccagaaaact
     3061 gtgtttaacc actggtcgcg aaatccccac catgtcgcac tcctgaaagg catcgtaacc
     3121 tatcaacgag gtcgctacct gcccggaaag gacaactaat ggaatggaat ccatataagc
     3181 ggtggcgatg ccagtaatcg cattggtcgc ccctggaccc gacgttacca gcacgacgcc
     3241 gacttccccg gtcgcgcgcg ccagaccatc ggccatatgc actgccgcct gctcatgacg
     3301 aactaaaaca tgatcgatac cacccacggt atgcaatgca tcgtaaatat caaggactgc
     3361 gcctccggga taaccgaata cttgtttaac gccctgatcg ataagcgatc ggacgaccat
     3421 ctcggctcca gacaacatct ccatggcctg cctcactgtt tgacggaaaa aatgtgtaaa
     3481 acacattaac cgtccgatct tgcttaagca attggcagcc agagacataa acgcagggta
     3541 ataagaataa acaggaggaa aggtgaaaag aaacgataaa atcctccatt acgaaggatt
     3601 aaattgataa taaatcggct gaatcccaca acttacagca atttctctat ttttttaatt
     3661 aatcctaaaa tagagagccc acacattcag aataaaattc tacattcaat aaaaaataca
     3721 gaataaacca gacattcatg tctgacctat tctgcattca gttagcgttt gcaaattgag
     3781 actaattgct cttccatcca ctgatggcct ttatcgcgcc cggcagcttc atgccaggag
     3841 agataacagg ttctgctatt ttgttttaac ggcagcggta atacctgtaa ttctaaggat
     3901 tcagcgaact cttcagccag ccaacgcggc gcaatagcga ccaaatgcgt ttgcgacacc
     3961 acgctaagta cgctcatcat tgccatgccc tgatacgcga tactggcttg cttatctacc
     4021 gtgtcatacc aaggttgact aaatgacgcg aaacgatcga gcgaaaccgc cgcatgttgt
     4081 tcgttataaa catcatgttt cagtaacggg cccttaattg ttggatgatt tttgctggct
     4141 accagcacca tttcatcttt aaataatggc acgctggtaa attcaggacg atggaagtct
     4201 tcataactaa tcacaaactc cgtttcctga taacgcagct gatgttcagt gttctgattt
     4261 aatgaagact tgaacataac atgtatattt ggcgcaatct gctcaatgtg attataaatc
     4321 tgcgaggtca gaatgctgtc taacgggctg caaacacaaa gatgaaatac acgttcactg
     4381 ctcgcgggtt caaaacctga accaggcaat tcattttgta ctagttgcaa tgcctgacga
     4441 actgagccaa aaagttgaaa tgcgcgagca gtcggttgaa taccacggcc ataacgaaca
     4501 aaaagctcgt cattaaacat caccttcagg cgtgcaacag cgttactgac cgcaggttgc
     4561 gacataccca gaacatgagc ggcacgggta atgttttgct cctgcatcac ggcatcgaaa
     4621 acggttaata agttgagatc gaccatgcgt agctgtggct tgcttaactc cgccgtctct
     4681 gaatgatctg tttgtacctc tggcatactt aactccactg tcacacttaa ctccctttcc
     4741 cttattggaa tgcagagaaa taatcctgaa gaatatgatt tatcatgcat ataaaataag
     4801 aaaaagcaaa atgagtaaaa ttcggaaaac ataaagatac gggttctgat aaaaacagaa
     4861 tccattgccg tggaatgagt catttaccac agcataagaa tccataatgt ttttgttgcg
     4921 aaaacaatct aatcataact accgcgaata ctcaatcatc tacaaaatgg attaaatgtg
     4981 cttatttaat aattaattta tatatttaat gcattaattc ttaacattaa ttgatcaata
     5041 atattcacca aatcaatatc aaaaaaaatc gcaaaacata taattcaata caaatcatca
     5101 ggataggttt tgcaacgcgt gcattttgtc ccctttttcc tcgttgatta gatgcaaaat
     5161 ttatgctgaa atatgtcaac cgatgaaaag cgtcggtagt taagcagaaa ttaatatcgc
     5221 ttactttaac caccgcagca caattagcta attttacgga tgcagaactc acgctggcgg
     5281 gacgttttta ttgcgtcagg gttgacatcc gtttttgtat ccagtaactc taaaagcata
     5341 tcgcattcat ctggagctga tttaatgact cacatcgttc gctttatcgg tctactacta
     5401 ctaaacgcat cttctttgcg cggtagacga gtgagcggca tccagcatta agccagcacg
     5461 cagtcaaaca aaaaacccgc gccattgcgc gggttttttt atgcccgaag cgaggcgctc
     5521 taaaagagac aaggacctaa accatgagcc agcaagtcat tattttcgat accacattgc
     5581 gcgacggtga acaggcgtta caggcaagct tgagtgtgaa agaaaaactg caaattgcgc
     5641 tggcccttga gcgtatgggt gttgacgtga tggaagtcgg tttccccgtc tcttcgccgg
     5701 gcgattttga atcggtgcaa accatcgccc gccaggttaa aaacagccgc gtatgtgcgt
     5761 tagctcgctg cgtggaaaaa gatatcgacg tggcggccga atccctgaaa gtcgccgaag
     5821 ccttccgtat tcataccttt attgctacct ccccaatgca catcgccacc aagctgcgca
     5881 gcaccctgga cgaggtgatc gaacgcgcta tctatatggt gaaacgcgcc cgtaattaca
     5941 ccgatgatgt tgaattttct tgcgaagatg ccgggcgtac acccattgcc gatctggcgc
     6001 gagtggtcga aacggcgatt aatgccggtg ccaccaccat caacattccg gacaccgtgg
     6061 gctacaccat gccgtttgag ttcgccggaa tcatcagcgg cctgtatgaa cgcgtaccta
     6121 acatcgacaa agccattatt tccgtacata cccacgacga tttaggcctg gcggtcggca
     6181 actcactggc ggcggtacat gccggtgcac gccaggtgga aggtgcaatg aacgggatcg
     6241 gcgagcgtgc cggtaactgt tcgctggaag aagtcatcat ggcgattaaa gttcgtaagg
     6301 atattctcaa cgtccacacc gccattaatc accaggagat atggcgcacc agccagttag
     6361 ttagccagat ttgtaatatg ccgatcccgg caaacaaagc cattgttggc agcagcgcat
     6421 tcgctcactc ctccggtatc caccaggatg gcgtgctgaa aaaccgcgaa aactacgaaa
     6481 tcatgacacc agaatctatt ggtctgaacc aaatccagct gaatctgacc tctcgttcgg
     6541 ggcgtgcggc ggtgaaacat cgcatggatg agatggggta taaagaaagt gaatataatt
     6601 tagacaattt gtacgatgct ttcctgaagc tggcggacaa aaaaggtcag gtgtttgatt
     6661 acgatctgga ggcgctggcc ttcatcggta agcagcaaga agagccggag catttccgtc
     6721 tggattactt cagcgtgcag tctggctcta acgatatcgc caccgccgcc gtcaaactgg
     6781 cctgtggcga agaagtcaaa gcagaagccg ccaacggtaa cggtccggtc gatgccgtct
     6841 atcaggcaat taaccgcatc actgactata acgtcgaact ggtgaaatac agcctgaccg
     6901 ccaaaggtca cggtaaagat gcgctgggtc aggtggatat tgtcgccaac tacaacggtc
     6961 gccgcttcca cggcgtcggc ctggccaccg atattgtcga gtcctccgcc aaagccatgg
     7021 tgcacgtact gaacaatatc tggcgtgccg cagaagtcga aaaagagttg caacgcaaag
     7081 ctcaacacaa cgaaaacaac aaggaaaccg tgtgatgtcg aagaattacc atattgccgt
     7141 attgccgggg gacggtattg gtccggaagt gatgacccag gcgctgaaag tgctgaatgc
     7201 cgtgcgcaac cgctttgcga tgcgcatcac caccagccat tacgatgtag gcggcgcagc
     7261 cattgataac cacgggcaac cactgccgcc tgcgacggtt gaaggttgtg agcaagccga
     7321 tgccgtgctg tttggctcgg taggcggccc gaagtgggaa catttaccac cagaccagca
     7381 accagaacgc ggcgcgctgc tgcctctgcg taagcgcttc aaattattca gcaacctgcg
     7441 cccggcaaaa ctgtatcagg ggctggaagc attctgtccg ctgcgtgcag acattgccgc
     7501 taacggcttc gacatcctgt gcgtgcgcga actgaccggc ggcatctatt tcggtcagcc
     7561 aaaaggccgc gaaggtagcg gacaatatga aaaagcgttt gataccgagg tgtatcaccg
     7621 ttttgagatc gagcgtattg cccgcatcgc gtttgaatct gctcgtaaac gtcgccacaa
     7681 agtcacctca atcgacaaag ctaacgtgct gcaatcctct attttatggc gtgaaatcgt
     7741 caacgagatc gccacggaat acccggatgt cgaactggcg catatgtaca tcgacaacgc
     7801 caccatgcag ctgattaaag atccatcaca gtttgacgtt ctgctgtgct ccaacctgtt
     7861 tggcgacatt ctgtctgacg agtgcgcaat gatcactggc tcgatgggga tgttgccttc
     7921 cgccagcctg aacgagcaag gttttggtct gtatgaaccg gcaggcggct cagcaccaga
     7981 tatcgcaggc aaaaacatcg ccaacccgat tgcgcaaatt ctgtcgctgg cactgctgct
     8041 gcgctacagc ctggatgccg atgatgcggc ttgcgccatt gaacgcgcca ttaaccgcgc
     8101 attagaagaa ggcattcgca ccggggattt agcccgtggc gctgccgccg ttagtaccga
     8161 tgaaatgggc gatatcattg cccgctatgt ggcagaaggg gtgtaatcat ggctaaaacg
     8221 ttatacgaaa aattattcga cgctcacgta gtgtacgaag ccgaaaacga aaccccgctg
     8281 ttatatatcg accgccacct ggtgcatgaa gtgacctcac cgcaggcgtt cgatggtctg
     8341 cgcgcccacg gtcgcccggt acgtcagccg ggcaaaacct tcgccactat ggatcacaac
     8401 gtctctaccc agacgaaaga cattaatgcc tgtggtgaaa tggcgcgcat ccagatgcag
     8461 gagctgatta aaaactgcaa agaatttggc gtcgaactgt atgacctgaa tcacccgtat
     8521 caggggatcg tccacgtaat ggggccggaa cagggcgtca ccttgccggg gatgaccatt
     8581 gtctgcggcg actcgcatac cgccacccac ggcgcgtttg gcgcactggc ctttggtatc
     8641 ggcacttccg aagttgaaca cgtactggca acgcaaaccc tgaaacaggg ccgcgcaaaa
     8701 accatgaaaa ttgaagtcca gggcaaagcc gcgccgggca ttaccgcaaa agatatcgtg
     8761 ctggcaatta tcggtaaaac cggtagcgca ggcggcaccg ggcatgtggt ggagttttgc
     8821 ggcgaagcaa tccgtgattt aagcatggaa ggtcgtatga ccctgtgcaa tatggcaatc
     8881 gaaatgggcg caaaagccgg tctggttgca ccggacgaaa ccacctttaa ctatgtcaaa
     8941 ggccgtttgc atgcgccgaa aggcaaagat ttcgacgacg ccgttgccta ctggaaaacc
     9001 ctgcaaaccg acgaaggcgc aactttcgat accgttgtca ctctgcaagc agaagaaatt
     9061 tcaccgcagg tcacctgggg caccaatcca ggccaggtga tttccgtgaa cgacaatatt
     9121 cccgatccgg cttcgtttgc cgatccggtt aaacgcgcgt cggcagaaaa agcgctggcc
     9181 tatatggggc tgaaaccggg cattccgctg accgaagtgg ctatcgacaa agtgtttatc
     9241 ggttcctgta ccaactcgcg cattgaagat ttacgcgcgg cagcggagat cgccaaaggg
     9301 cgaaaagtcg cgccaggcgt gcaggcactg gtggttcccg gctctggccc ggtaaaagcc
     9361 caggcggaag cggaaggtct ggataaaatc tttattgaag ccggttttga atggcgcttg
     9421 cctggctgct caatgtgtct ggcgatgaac aacgaccgtc tgaatccggg cgaacgttgt
     9481 gcatccacca gcaaccgtaa ctttgaaggc cgccaggggc gcggcgggcg cacgcatctg
     9541 gtcagcccgg caatggctgc cgctgctgct gtgaccggac atttcgccga cattcgcaac
     9601 attaaataag gagcacacca tggcagagaa atttatcaaa cacacaggcc tggtggttcc
     9661 gctggatgcc gccaatgtcg ataccgatgc aatcatcccg aaacagtttt tgcagaaagt
     9721 gacccgtacg ggttttggcg cgcatctgtt taacgactgg cgttttctcg atgaaaaagg
     9781 ccaacagcca aacccggact tcgtgctgaa cttcccgcag tatcagggcg cttccatttt
     9841 gctggcacga gaaaacttcg gctgtggctc ctcgcgtgag cacgcgccct gggcattgac
     9901 cgactacggc tttaaagtgg tgattgcgcc gagttttgct gacatcttct acggcaatag
     9961 ctttaacaac cagctgctgc cggtgaaatt aagcgatgca gaagtggacg aactgtttgc
    10021 gctggtgaaa gccaatccgg ggatccattt cgacgtggat ctggaagcgc aagaggtgaa
    10081 agcgggagag aaaacctatc gctttaccat cgatgccttc cgccgccact gcatgatgaa
    10141 cggtctggac agtattgggc ttaccttgca gcacgacgac gccattgccg cttatgaagc
    10201 aaaacaacct gcgtttatga attaatcccc ttgcccggtc aaatgaccgg gctttccgct
    10261 atcgtccacg tcatcaaacg tctttaacct ttgcggttaa aaataatgcg acaacagaaa
    10321 taaccgcaat tacccagtag a
//
LOCUS       DAKISA010000137        10263 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.137, whole genome shotgun sequence.
ACCESSION   DAKISA010000137 DAKISA010000000
VERSION     DAKISA010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10263
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.137"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            142..2010
                     /gene="kup"
                     /locus_tag="OVW45_003776"
     CDS             142..2010
                     /gene="kup"
                     /locus_tag="OVW45_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCV5528118.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAMHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2177..2596
                     /gene="rbsD"
                     /locus_tag="OVW45_003777"
     CDS             2177..2596
                     /gene="rbsD"
                     /locus_tag="OVW45_003777"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCV5528119.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2604..4109
                     /gene="rbsA"
                     /locus_tag="OVW45_003778"
     CDS             2604..4109
                     /gene="rbsA"
                     /locus_tag="OVW45_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HCV5528120.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVIALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLISQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLCFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            4114..5079
                     /gene="rbsC"
                     /locus_tag="OVW45_003779"
     CDS             4114..5079
                     /gene="rbsC"
                     /locus_tag="OVW45_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCV5528121.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5104..5990
                     /gene="rbsB"
                     /locus_tag="OVW45_003780"
                     /pseudo
     CDS             5104..5990
                     /gene="rbsB"
                     /locus_tag="OVW45_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6116..7045
                     /gene="rbsK"
                     /locus_tag="OVW45_003781"
     CDS             6116..7045
                     /gene="rbsK"
                     /locus_tag="OVW45_003781"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCV5528122.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVLGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7049..8041
                     /gene="rbsR"
                     /locus_tag="OVW45_003782"
     CDS             7049..8041
                     /gene="rbsR"
                     /locus_tag="OVW45_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCV5528123.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="OVW45_003783"
     CDS             complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="OVW45_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCV5528124.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVSFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(9457..10149)
                     /locus_tag="OVW45_003784"
     CDS             complement(9457..10149)
                     /locus_tag="OVW45_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCV5528125.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLNLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         2492 a         2472 c         2720 g         2579 t
ORIGIN      
        1 aagggggggt atttttcttt gagccaggtt aatgtggccg catttaggag tacgattttg
       61 ccgttaatcg tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat
      121 atttcaacga aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc
      181 ctcgcggcga ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt
      241 gaatgtttgt ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggcttttta
      301 tcgctgatct tctggctgct aatctttgtg gtttccatta aatatctcac cttcgtgatg
      361 cgggcagata acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat
      421 acgtcggcgc gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc
      481 tatggtgaag tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa
      541 atcgtcgccc cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta
      601 ttatttatga ttcaaaagca tggcacggct atggtcggta agctgtttgc gccgatcatg
      661 ctgacctggt ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa
      721 gtgctgcatg cgctgaatcc gatgtgggcg atgcatttct tccttgaata caaaacggtt
      781 tcttttattg cattaggggc agtggtgttg tcgattacgg gggtcgaggc gctgtatgct
      841 gatatggggc actttggtaa gttccctatt cgcctggcgt ggttcaccgt cgtattacct
      901 tccttgaccc ttaattactt cggtcaggga gcgctgttgt taaagaaccc ggaagcgatt
      961 aagaacccgt tcttcctgtt ggcaccggac tgggcgctga tcccgctgct gatcatcgcc
     1021 gcactggcga cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt
     1081 caggcggtac gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag
     1141 tcagggcaaa tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg
     1201 attgtcagct ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga
     1261 accatggtgc tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat
     1321 aagtattttg ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc
     1381 gctaacctcg ataaactgct ctccggcggc tggttgccat tgagcctcgg tactgtgatg
     1441 tttatcgtga tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa
     1501 catggtaact ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg
     1561 cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat
     1621 aaccttaaac ataacaaggt attgcatgag agggtgattc tgttaactct gcgcaccgaa
     1681 gacgcgccat atgtccataa cgtccgtcgg gtacagattg aacaactgtc gcccactttc
     1741 tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac
     1801 cgctgcggtc tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat
     1861 gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg
     1921 ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt
     1981 atcgaactgg gtactcaggt cgaaatctaa cgctagacgc ctcctttctc cataaggggg
     2041 cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt
     2101 aacgtcacga tggttttccc aactcagtca ggattaaact gtgggtcagc gaaacgtttc
     2161 gctgatggag aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat
     2221 ctcccgtctg ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa
     2281 aagtacaacg cgtatcgata tggcattaac ccagggtgta ccttctttta tgcaggtgct
     2341 gggcgtcgtc acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag agatcaaaca
     2401 acataatccg caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca
     2461 gggaaatacc attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga
     2521 aagtcaggcg gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc
     2581 tggcgtgacg ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag
     2641 ccttcccggg cgtaaaagcc ctctcgggcg ctgcgttaaa tgtctatccg ggccgcgtga
     2701 tagcgctggt gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg cttactggta
     2761 tctatactcg cgatgccggc acgcttttat ggctggggaa agaaacgaca tttaccgggc
     2821 cgaaatcttc ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatctcgc
     2881 agttgaccat tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa
     2941 ttgactggaa aaccatgtat gccgaagcgg ataaattgct ggctaaactt aacctgtgct
     3001 ttaaaagcga caagctggtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg
     3061 ccaaagtgct gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga
     3121 ccgataccga aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccgcg
     3181 gtattgtcta tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg
     3241 tttttcgtga cgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc
     3301 tgattgagat gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc
     3361 cgggagatat ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt
     3421 ttactttacg caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg
     3481 aactgatgaa agtgctctac ggcgcactgc cgcgcaccag cggttacgtc accctggatg
     3541 ggcatgaagt cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct
     3601 ccgaagaccg taaacgtgac ggtttagtgt tgggcatgtc agtaaaagag aacatgtcgc
     3661 tgacagcgct gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc
     3721 aggcagtgag tgatttcatt cgtctgttta atgtgaaaac tccgtcgatg gaacaggcaa
     3781 ttggtctgct ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac
     3841 gccccaaagt gttgatcctt gatgaaccta cccgtggcgt agatgtcggc gcgaaaaaag
     3901 agatctatca actgattaac cagttcaaag ccgatggctt gagcatcatt ctggtgtcat
     3961 cggagatgcc agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc
     4021 tcagcgggga atttactcgt gagcaggcca cccaggaagt gttaatggct gccgctgtgg
     4081 gcaagcttaa tcgcgtgaat caggagtaaa aaaatgacaa cccagactgt ctctggtcgc
     4141 cgttatttca cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg
     4201 atcgcgattg tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt
     4261 ctccagcaaa cctcggtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg
     4321 tcgggcatcg acttgtctgt aggttctctg ttggcgctaa ccggcgcagt tgctgcatct
     4381 atcgtcggca ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcgc gttaggtgcc
     4441 gcaattggtg cggtaaccgg ggtgattgta gcgaaaggtc gcgttcaggc gtttatcgct
     4501 acgctggtta tgatgctttt actgcgcggc gtgaccatgg tttataccaa cggtagccca
     4561 gtgaataccg gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg
     4621 ctgggcgtac cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg
     4681 ctgcatcaca cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg
     4741 cgtctttctg gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg
     4801 ctggcatcgc tggccgggat cattgaagtg gcgcgtctct cctccgcaca acccacggcg
     4861 gggactggct atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg
     4921 ggcggaaaag gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat
     4981 aatggattga atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg
     5041 attttgctgg cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg
     5101 aatatgaaca tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc
     5161 agtgcgaatg cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg
     5221 ttctttgtat cgctgaaaga tggcgcgcag aaagaggcgg ataagcttgg ctataacctg
     5281 gtggtgctgg actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc
     5341 gttcgcggca ccaaaattct gctgattaac ccgaccgact ccgacgcagt aggtaatgct
     5401 gtgaagatgg ctaaccaggc gaacatcccg gttatcactc ttgaccgcca ggcaacgaaa
     5461 agtgaagtgg tgagccacat tgcttctgat aacgtactgg gtggcaaaat cgccggtgat
     5521 tacatcgcga agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgctggt
     5581 acatccgcag cccgtgaacg tggcgaaggc ttccagcagg ccgttgctgc tcacaagttt
     5641 aatgttcttg ccagccagca gattttgatc gcactaaagg tttgaacgta atgcagaacc
     5701 tgttgaccgc tcatccggat gttcaggctg tattcgcgca gaatgatgaa atggcgctgg
     5761 gcgcgctgcg cgcactgcaa actgccggta aatcggatgt gatggtcgtc ggatttgacg
     5821 gtacaccgga tggcgaaaaa gcggtgaatg atggcaaact agcagcgact atcgctcagc
     5881 tacccgatca gattggcgcg aaaggcgtcg aaaccgcaga taaagtgctg aaaggcgaga
     5941 aagttcaggc taagtatccg gttgatctga aactggttgt taagcagtag ttttaatcag
     6001 gttgtatgac ctgatggtga cataaatacg tcatcgacag atgaacgtgt aatataaaga
     6061 aaagcagggc acgcgccacc ctaacacggt ggcgcatttt atggacatcc cgaatatgca
     6121 aaacgcaggc agcctcgttg ttcttggcag cattaatgct gaccacattc ttaatcttca
     6181 atcttttcct actccaggcg aaaccgtaac cggtaaccac tatcaggttg catttggcgg
     6241 caaaggcgcg aatcaggctg tggctgctgg gcgtagcggt gcgaatatcg cgtttattgc
     6301 ctgtacgggt gatgacagca ttggtgagag cgttcgccag cagctcgcca ctgataacat
     6361 tgatattact ccggtcagcg tgatcaaagg cgaatcaaca ggtgtggcgc tgatttttgt
     6421 taatggtgaa ggtgagaatg tcatcggtat tcatgccggc gctaatgctg ccctttcccc
     6481 ggcgctggtg gaagcgcaac gtgagcgtat tgccaacgcg tcagcattat taatgcagct
     6541 ggaatcacca ctcgaaagtg tgatggcagc ggcgaaaatc gcccatcaaa ataagactat
     6601 cgttgcgctt aacccggctc cggctcgcga acttcctgac gaactgctgg cgctggtgga
     6661 cattattacg ccaaacgaaa cggaagcaga aaagctcacc ggtattcgtg ttgaaaatga
     6721 tgaagatgca gcgaaggcgg cgcaggtact gcatgaaaaa ggtatccgta ctgtactgat
     6781 tactttagga agtcgtggtg tatgggctag cgtgaatggt gaaggtcagc gcgttcttgg
     6841 attccgggtg caggctgtcg ataccattgc tgccggagat acctttaacg gcgcgttaat
     6901 cacggcgttg ctggaagaaa aaccattgcc agaggcgatt cggtttgccc atgctgccgc
     6961 tgcgattgcc gtaacacgta aaggcgcaca accttccgta ccgtggcgtg aagagatcga
     7021 cgcattttta gacaggcaga ggtgacgctt ggctacaatg aaagatgttg cccgcctggc
     7081 gggcgtttct acctcaacag tttctcacgt tatcaataaa gatcgcttcg tcagtgaagc
     7141 gattaccgcc aaagttgaag cggcgattaa agaactcaat tacgcgccat cagccctggc
     7201 gcgtagcctc aaactcaatc aaacacatac cattggcatg ttgatcactg ccagtaccaa
     7261 tcctttctat tcagaactgg tgcgtggcgt tgaacgcagc tgcttcgaac gcggttatag
     7321 tctcgtcctt tgcaataccg aaggcgatga acagcggatg aatcgcaatc tggaaacgct
     7381 gatgcaaaaa cgcgttgatg gcttgctgtt actgtgcacc gaaacgcatc aaccttcgcg
     7441 tgaaatcatg caacgttatc cgacagtgcc tactgtgatg atggactggg ctccgttcga
     7501 tggcgacagc gatcttattc aggataactc gttgttgggc ggagacttag caacgcaata
     7561 tctgatcgat aaaggtcata cccgtatcgc ctgtattacc ggtccgctgg ataaaactcc
     7621 ggcgcgcctg cggttggaag gttatcgggc ggcgatgaaa cgtgcgggtc tcaacattcc
     7681 tgatggctat gaagtcactg gtgattttga atttaacggc gggtttgacg ccatgcgcca
     7741 actgttatca catccgctgc gtcctcaggc cgtctttacc ggaaatgacg ctatggctgt
     7801 tggcgtttac caggcgttat atcaggcaga gttacaggtt ccgcaggata tcgcggtgat
     7861 tggctatgac gatatcgaac tggcaagctt tatgacgcca ccattaacca ctatccacca
     7921 accgaaagat gaactggggg agctggcgat tgatgtactc atccatcgga taacccagcc
     7981 gacccttcag caacaacgat tacaacttac tccgattctg atggaacgcg gttcggctta
     8041 gatttacgct gtcttttgat caaattatta ccatcggttg ttttcagaag catgaacatt
     8101 gctgctgaag caacagtaat aatgcccatc gtgataaacg tatagtggaa ttgttcgaca
     8161 gtcgttgtgc cttccatccc ttcataaacg cgaaggacgg ccgcacttac agcaacgcct
     8221 aaactaatcg acagttgctg cgtgaccgcc agaacactgt taccgctgct ggcgttgtca
     8281 tcggtcagat cggcaagtgt gatggtattc atcgcggtaa attgcgtcga catagccatc
     8341 cctaatataa acaacggcaa gatcagcatc catatcgcca ttgccggtga ttgcaaagag
     8401 aactgagcga tcattagccc aataatcacc gtgatcccca ctaacgtatg gcgatagccc
     8461 agacgacgta agacttgggt aaccatcgat tttgcaataa tggaacctaa cgctgtcggt
     8521 gccatcatac agccggcaat aaacgcctga taaccaaatc ctacctgtaa catcaatggc
     8581 ataaggaacg atacacaacc ggtccccaga cgggttgcaa tattgcctac gataccgatt
     8641 gagaaagtgc gggttttaaa taaatctaat gaaattaatg ggtttggcgt gtgtcgcgca
     8701 tggagaatat agagaagcag taacccgatg ctggtgacaa ttaccgtcaa ggcaatccag
     8761 ctggcgacaa tcttttcccc gaatagctct attccgcttg agaagagaac aaggctgagg
     8821 ccaaacagca aaaagccagt gatatcgaat ctgcgtcgtg cggtggtgaa attgggcata
     8881 tgtttgcgcg cgtaaagaag gcccgcaata cctatgggga tattgattaa aaatatccag
     8941 tgccaggttg cccaggtgac cagcacgccg ccaagaacgg ggcctaaaat tggccccacc
     9001 agacccggca tggcgacaaa attcaatact ggaagaagtt cattacgagg ataagcgcgc
     9061 agtaaggcca gccgagcaac aggcatcatc attgcgccgc ctatcccctg aataacccgg
     9121 aagacaacca gctgtggtag cgaattagaa agtgcgcagg ccagagaacc caatgtgaac
     9181 agactcacgg caagggtaaa aatgcgacgc gtaccgaagc gatcggctag ccatccgctt
     9241 accggaataa gcatcgccac cgtcagcgta taactgatga tggctgattg catcgcgaga
     9301 ggagaacgat taaggctatg agcgattgcg ggtaaggcgg tattcagaat agtggcatca
     9361 agtgcctgca tgaagaaggc catcgccgcg atccacggca aacccgccat actgcgcttc
     9421 tttttatcgc tcattcaatg tcttgttatc gggttgtcac ttatcaggtg agcgtagcag
     9481 cgcctgacaa gctttaaatg ccgcgtcgcc atcgctttgg acaatcgcat cgacaatcgc
     9541 ctggtgcaga tttagcttta tcactgtgtc gctggtaatt gacgtgaagt aagtgtgata
     9601 aaccgaatgg aatagcgagg cgaatgaggt caaaaacgga ttggcgctca tttcatagat
     9661 atgctcatgc caggccatat cgacttcgat ccagcgttca cggcgaaagt tctcttttaa
     9721 tgccgccatt tcggccatta acgtattgag atgcgccttc tgttccgcgg tgccaaccgt
     9781 tgctgccagt aggcaggctt gcggctccag acagatacgc ataaccagaa agtgatcgat
     9841 gacctgatga aagttctctt ctgtcatcca ccaggtaagc aattcctgat caagaaaatt
     9901 ccagttcgat tgtggcatga cccgagtacc aattcgcggt cgcggtaaaa ccatcccttt
     9961 tgccgttaac gttttgaccg cttcgcgtac cgctgtacga ctcactccaa attgctcgcc
    10021 cagctcaatc tcaccaggca aaatggtgcc gggttcatat tcacctttta agatccgttg
    10081 cgccagcttc tcagccagaa catacgaaag gtttttctgt gcagctaact gttgtgcgct
    10141 taaaggcatt acttatcttc ctttttcttt ttattcctcc ttagtatgcc accaggaagt
    10201 gtgattacgg ttgcaaaaac ggcaaattgc ttgttttatg gtacattaac ggggcttttg
    10261 ctg
//
LOCUS       DAKISA010000138        10205 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.138, whole genome shotgun sequence.
ACCESSION   DAKISA010000138 DAKISA010000000
VERSION     DAKISA010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10205
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.138"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..2038
                     /gene="speF"
                     /locus_tag="OVW45_003785"
     CDS             <1..2038
                     /gene="speF"
                     /locus_tag="OVW45_003785"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HCV5528126.1"
                     /translation="GYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLET
                     AASHYETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGETLFR
                     SDLCNADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPG
                     DLVLFDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEV
                     APQRAKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMM
                     ADCSPLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNA
                     FMMHASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFV
                     PELVDGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGID
                     ARNGEYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRF
                     EKLLESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKAH
                     FPHVSMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVL
                     RYFSALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2035..3354
                     /gene="potE"
                     /locus_tag="OVW45_003786"
     CDS             2035..3354
                     /gene="potE"
                     /locus_tag="OVW45_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCV5528127.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(3410..5050)
                     /gene="pgm"
                     /locus_tag="OVW45_003787"
     CDS             complement(3410..5050)
                     /gene="pgm"
                     /locus_tag="OVW45_003787"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCV5528128.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQQFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5076..5621)
                     /gene="seqA"
                     /locus_tag="OVW45_003788"
     CDS             complement(5076..5621)
                     /gene="seqA"
                     /locus_tag="OVW45_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCV5528129.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            5806..6570
                     /gene="ybfF"
                     /locus_tag="OVW45_003789"
     CDS             5806..6570
                     /gene="ybfF"
                     /locus_tag="OVW45_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCV5528130.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMHQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            6710..7003
                     /gene="ybfE"
                     /locus_tag="OVW45_003790"
     CDS             6710..7003
                     /gene="ybfE"
                     /locus_tag="OVW45_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCV5528131.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7143..7673
                     /gene="fldA"
                     /locus_tag="OVW45_003791"
     CDS             7143..7673
                     /gene="fldA"
                     /locus_tag="OVW45_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCV5528132.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            7883..7969
                     /gene="uof"
                     /locus_tag="OVW45_003792"
     CDS             7883..7969
                     /gene="uof"
                     /locus_tag="OVW45_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCV5528133.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            7962..8408
                     /gene="fur"
                     /locus_tag="OVW45_003793"
     CDS             7962..8408
                     /gene="fur"
                     /locus_tag="OVW45_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCV5528134.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(8493..8819)
                     /locus_tag="OVW45_003794"
     CDS             complement(8493..8819)
                     /locus_tag="OVW45_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528135.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(8869..10205)
                     /gene="chiP"
                     /locus_tag="OVW45_003795"
                     /pseudo
     CDS             complement(8869..10205)
                     /gene="chiP"
                     /locus_tag="OVW45_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="chitoporin"
BASE COUNT         2423 a         2559 c         2601 g         2622 t
ORIGIN      
        1 tggctatggc attcctgttt ttattgcaac ggaaaatgaa gaacgtgtac cagcagaata
       61 tctgccacgt atttctggtg tcttcgagca ttgcgaatca cggaaagagt tttatggacg
      121 tcagttagaa accgctgcta gccattatga aacgcaactg cgcccgccct tcttccgcgc
      181 actggtcgat tatgtcaatc agggcaatag cgcttttgac tgcccgggtc atcagggagg
      241 tgagtttttc cgtcgtcatc cggcagggaa tcagttcgtg gagtacttcg gagaaacgct
      301 gttccgttcc gacttatgca acgcagacgt agcaatgggc gatctgctga ttcacgaagg
      361 cgcgccatgc attgcacagc aacatgcggc aaaagtgttt aatgccgata aaacatactt
      421 cgttctaaat ggcacttcat cttctaacaa agtggtttta aacgccctgc taacaccggg
      481 tgatctggtg ctgtttgatc gcaataacca caaatctaac caccacggag cgttgctaca
      541 ggctggtgca acaccggttt atctggaaac ggcacgtaac ccgtatggct ttatcggtgg
      601 cattgatgcg cactgttttg aagaaagtta cctgcgtgaa ctgatcgcgg aagtcgcacc
      661 gcagcgggca aaagaggctc gtcctttccg cctcgctgtg attcagttag gcacctacga
      721 cggtacgatt tataacgccc gccaggtggt ggataaaatt ggtcatctgt gtgactacat
      781 tctgtttgac tccgcatggg ttggctatga gcaatttatc ccgatgatgg ctgactgttc
      841 gccgctgttg ctggatctta atgagaacga tccgggtatt ctggttacgc aatccgtaca
      901 taaacagcag gctggttttt ctcaaacttc acaaatccat aaaaaagaca gccacatcaa
      961 agggcaacag cgttatgtac cgcacaaacg catgaacaac gcctttatga tgcacgcctc
     1021 caccagcccg ttctatccgc tgtttgccgc actggatatc aacgccaaaa tgcatgaagg
     1081 tgtcagcggt cgtaatatgt ggatggattg tgtggtaaat ggcattaatg cccgcaaact
     1141 gatcctcgat aactgtcagc atattcgtcc gttcgtacct gaactggtgg atggtaaacc
     1201 ctggcagtcg tatgaaacag cgcaaattgc ggttgatctg cgcttcttcc agtttgtacc
     1261 aggggaacac tggcattctt ttgaaggcta tgcagagaat caatactttg tcgatccttg
     1321 caaactgttg ctgacaaccc caggtattga tgcacgtaac ggcgaatatg aagcgttcgg
     1381 tgtacccgcg acgattcttg ctaacttcct gcgcgaaaat ggcgtagtgc cggaaaaatg
     1441 cgatcttaac tccatcctct tcctgctgac tccggcagaa gatatggcca aacttcagca
     1501 acttgttgcc ctgctggtac gcttcgaaaa actgcttgag tccgacgcgc cattagcaga
     1561 agtgctgcct tccatctaca aacagcatga agagcgctac gccggttata ccctgcgtca
     1621 gttgtgccag gaaatgcatg atttgtatgc ccgccacaac gtgaaacaac tgcaaaaaga
     1681 gatgttccgc aaagcgcact tcccacacgt cagcatgaat ccgcaagaag ccaactacgc
     1741 ctatttacgc ggtgaagtgg agctggttcg tctgccggat gccgaaggtc gtatcgctgc
     1801 cgaaggggcg cttccttatc ctccgggtgt gctgtgtgtt gttccgggtg aaatctgggg
     1861 tggtgctgtt ttgcgttact tcagcgctct ggaagaaggg atcaacctgc tgccaggttt
     1921 tgcaccggag ctgcagggtg tctatatcga agaacatgat ggtcgtaagc aagtttggtg
     1981 ctatgtcatc aagcctcgtg atgcgcaaag caccctgttg aaaggggaaa aattatgagt
     2041 caggctaaat cgaacaaaat gggcgtcgtt cagttaacca tactgacgat ggtcaacatg
     2101 atgggctccg gtatcatcat gctgccgaca aagcttgccg aagttgggac aatttcaatt
     2161 atctcctggc tggtgacagc cgtaggctca atggcactgg catgggcatt cgcaaaatgc
     2221 ggtatgttca gccgtaaatc aggtggtatg ggcggttatg ccgagtatgc tttcggtaaa
     2281 tccggtaact ttatggcgaa ctatacctac ggcgtctcac tgctgattgc taacgtcgcg
     2341 attgctattt cggcggttgg ttacggcacc gaactgctcg gcgcgagttt gtcgccagtg
     2401 cagattggtc ttgcgaccat cggggtgctg tggatttgta ccgtggctaa ctttggtggt
     2461 gcgcgcatta ccgggcaaat cagtagcatt accgtgtggg gggtcattat tccggtggta
     2521 ggcctgtgca ttatcggctg gttctggttt agcccgacgc tttacgttga ttcctggaat
     2581 ccgcatcatg caccgttctt cagtgcagta ggttcttcca tcgctatgac gctgtgggct
     2641 tttctcggtc tggagtctgc gtgtgcgaat actgatgtag tggaaaaccc ggaacgtaat
     2701 gtgccaatcg cggtactcgg cggtacgtta ggtgcggcgg tgatttatat cgtctccacc
     2761 aacgtgattg cagggattgt gcctaatatg gagctggcaa attcaacggc accatttggc
     2821 ctggccttcg cacagatgtt cacaccagaa gtgggtaaag tcattatggc gctgatggtg
     2881 atgtcctgct gcggttcgct gcttggctgg cagttcacca ttgcccaggt gtttaagtct
     2941 tcatctgatg aaggctactt ccctaaaatt ttctcccgtg taaccaaagt ggatgcaccg
     3001 gtgcagggaa tgttgaccat tgtgattatc cagagcgggt tggcgctgat gacgattagc
     3061 ccgtcgctaa acagtcagtt caacgtgctg gttaacctgg ccgtggtgac caatatcatt
     3121 ccgtatattc tgtcaatggc ggcattagtc attattcaga aggtcgctaa tgtgcccccc
     3181 tcaaaagcga aagttgcaaa ctttgttgct tttgttggcg cgatgtatag cttttatgcg
     3241 ctgtactcat ccggggaaga agccatgctg tacggttcca tcgtgacctt ccttggttgg
     3301 acactgtatg gtctggtatc accacgcttt gaactgaaaa ataaacacgg ttaaaaaagc
     3361 aaagtaaaaa agggcgatct tgcgaccgcc ctttttttat taaatgtgtt tacgcgtttt
     3421 tcagaacttc gctaacaatc tcaaccgctt ctttctcaat ctgcttgcga tgttcttcac
     3481 cgaggaagct ttcgcagtag atcttatatg cgtcttccgt gcctgacgga cgcgcggcga
     3541 accagccgtt gtcagtcatc actttcagac cgccgataga agcaccattg cccggtgccg
     3601 ccgtcaggcg cgcggtgatc gggtcgcctg ccagggtgct ggcgctcacc atttccggag
     3661 acagcttaga cagcgccgct ttttgtgcgg aagtcgcagc tgcctgcaaa cggttgtagc
     3721 tcggcgcacc aaagcgtttt gccagttcgt tgtagtgttc ctgcgggttc ttaccggtga
     3781 cagcggtgat ttccgccgcc agcagacaca tgatgatgcc gtctttgtcg gtggaccacg
     3841 gcgtgccgtc gaaacgcagg aaggaagccc ctgcgctctc ttcgccgcca aagccgaagc
     3901 tgccgtcgaa cagaccatca acaaaccatt tgaaacctac cgggacttct accagcttgc
     3961 ggcccaagtc attgaccaca cggtcgatca tcgcagaaga aaccagcgtt ttaccgacag
     4021 caacatcttt gccccactgc ggacgatgct ggaacaggta gttgatcgcc accgccaggt
     4081 agtggttcgg attcatcaaa cctgccggag tgacgatacc gtgacggtca taatccgggt
     4141 cgttagcaaa cgccagatcg aacttatcac gcaatgccag caggcccgcc atcgcacact
     4201 cggaggagca gtccatacgg atcgcgccgt ctttatcgag gtgcataaag cggaaggttt
     4261 gatcgacctg atcgttaacg atggtcaggt tgaggttgta atactcgcca attcgcttcc
     4321 agtattcgat accggaacca cccagcggat caacgcccag cgtcaggccc gctttctgaa
     4381 tcgccgccat atcaacgata tcggccagac cttccacaaa ctgctgcacc agatcctgct
     4441 ctttcacatg accggatgcc atcgcttcgt cgagggagat acgcttcacg cctttcaggc
     4501 catcggccat cagtgcgttg gcacgatctt ccaccacttt agtgacgttg gtatcagccg
     4561 ggccaccatt tggcggattg tacttgatac caccatcttc cggcgggtta tgggagggtg
     4621 taatcacgat accgtctgcc agcgggccac cttttttatt atgaaccagg atggcattgg
     4681 aaacggcagg cgtcggagtg aagccattgt tttcctgcac aatgacatca acgccgttcg
     4741 ctgccagcac ttccagtacg gaaatgaacg caggctcgga cagggcgtga gtatctttac
     4801 ccacatagca agggccagtg atgccgtttt tcgcacgttc ttcagcaatt gcctgagcga
     4861 tcgccagaat gtgcggctcg ttaaagctgt ggcgcgctgc actgccacgg tgaccggaag
     4921 taccgaattt caccgcgtgc tccgcattcc ctgcttctgg tttcagtaca taatattgcg
     4981 ccgtcagttg ggcgacgtta atcaaatcac tctgttgtgc aggttggcct gcacgattgt
     5041 ggattgccat tgctttgtcc tttgtctgca acgttttaga tagttccgca aaccttctca
     5101 atcaattccg ccgggaattg catcgactgc atgatgtgtt cgatcatgct gcatttacgg
     5161 ccggtgttgg tgttggtgat cacccaatac ggcgtgcctg gcacatgttt cggcttggtc
     5221 tgattaccat ttttcagcag cgtttgttca tctgccgcaa agtaaacgcg tgtacgaccg
     5281 tgcaacgatt ccgttgcttc ggcaaacgcc tgggcgtcaa gagaatatag tgtagacaac
     5341 agcagcataa agcgattgac cgctcgcttt tgctctgcgt attcgtcaga aagcagaagt
     5401 tcacgcattg cgcgaacctt gtctttaatc gttttgaccg gcttcgcttc gacgatagca
     5461 ggtgacgcaa cgcgaacctc tttcgtcacc ggagcagcag gctgtgatgc ggcggaaaat
     5521 ttcaacatac gccgtaaaat gtcggatgcg ctctcgccga tatgcttagt gtggctggca
     5581 atatagctgt agagttcatc atcaacttca atcgttttca tcttaatcca gtacagtgtc
     5641 ttagctgaat acaagtcgcc aagagtatat gaataaatcc cggcagcgga tagcgtcaag
     5701 cctggcttgc agaaaaactc ggaataaacc ttatttgttg cagccctgtg gcacaatggt
     5761 caacataata ccctaacccg acaacgcgaa aaaaagaact ttgccatgaa attgaatatc
     5821 cgcgcgcaaa ctgcacaaaa ccagcacaat aattctccca tcgttcttgt ccatggtctg
     5881 tttggtagcc tcgacaacct tggcgtactg gctcgcgatc tggtaaacga tcacaatatc
     5941 atccaggttg atatgcgtaa ccacggtctt tcaccgagag atccggtaat gaattacccg
     6001 gcgatggcgc aggatcttgt tgatactctg gatgcacagc agatcgacaa agcaacattt
     6061 atcggtcact ccatgggcgg taaagcggta atggcactta cggcactggc ccgcgatcgc
     6121 atcgataaac tggtggcgat cgatatcgca ccggtcgact atcacgtacg ccgtcatgat
     6181 gagattttcg cggctatcaa cgcggtcagt gaatcggacg cacaaactcg ccagcaagca
     6241 gcagcaataa tgcaccagca tcttaatgaa gaaggggtga ttcagtttct gttgaaatct
     6301 tttgttgacg gggagtggcg ctttaacgtg ccggtattgt gggatcagta tccgcatatt
     6361 gtgggttggg agaaaatccc ggcatgggat caccctgccc tgtttatccc tggcggcaat
     6421 tctccgtatg ttagcgagca gtaccgtgat gatttactgg cacaatttcc acaggcacgg
     6481 gcgcatgtga ttgcaggcgc gggtcactgg gtccatgctg aaaaaccgga tgcggtatta
     6541 cgcgctatcc gtcgctatct caatgattaa cgcaactgat taaaaaccca gcgtccgcgt
     6601 cgctttccgc gcgtgggcgt tggcgtgact catcgcgctg atgtattatg gcgcgctatc
     6661 catccgggca gaggcctggc tgattgtttc ccccgaagtc accaagatca tggccaaaga
     6721 acaaacggac cgtacgacat tagatctgtt cgcgcacgag cgtcgaccgg gacgaccgaa
     6781 aactaatccg ctttcgcgcg atgaacagct gcgtattaat aaacgcaacc agctaaaacg
     6841 cgacaaagta cgtggcctta agcgtgtcga actgaagctg aacgcggaag ctgtcgaggc
     6901 gctgaacgag ctggcggagt cgcgcaatat gagccgtagc gaactgatcg aagagatgtt
     6961 aatgcagcaa ctggcggcgc tgcgtagtca gggcatcgtt taaatttcca ctttcatgta
     7021 gcacagtgtg cagtcctgct cgtttgctga ttccgcaaga ctgcctgttc tgctatgatt
     7081 gcctttatcc gtgggcaatt ttccaccccc atttcaataa gtttcaagag gttatttcac
     7141 tcatggctat cactggcatc tttttcggca gcgacaccgg taataccgaa aatatcgcaa
     7201 aaatgattca aaaacagctt ggtaaagacg ttgccgatgt ccatgacatt gcaaaaagca
     7261 gcaaagaaga tctggaagct tatgacattc tgctgctggg catcccaacc tggtattatg
     7321 gcgaagcgca gtgtgactgg gatgacttct tcccgactct cgaagagatt gatttcaacg
     7381 gcaaactggt tgcgctgttt ggttgtggtg accaggaaga ttacgccgaa tatttctgcg
     7441 acgcattggg caccatccgc gacatcattg aaccgcgcgg tgcaacgatc gttggtcact
     7501 ggccaactgc gggctatcat ttcgaagcat caaaaggtct ggctgatgac gaccactttg
     7561 tcggtctggc tatcgacgaa gaccgtcagc cggaactgac cgctgaacgt gtagaaaaat
     7621 gggttaaaca gatttctgaa gagttgcatc tcgacgaaat tctcaatgcc tgatgtgatg
     7681 cggcgtagac tcatgtctac gccgtattaa tagataatgc caatcaaaat aattgctaca
     7741 aatttgtaac ttttgctgtt gtacctgtac aatgtcccgg tgttcaagtg gccttgccgt
     7801 tgtaaatgta agctgtgcca cgtttttatt aacaatattt gccagggact tgtggttttc
     7861 atttaggcgt ggcaattcta taatgatacg cattatctca agagcaaatt ctgtcacttc
     7921 ttctaatgaa gtgaaccgct tagtaacagg acagattccg catgactgat aacaataccg
     7981 ccctaaagaa agctggcctg aaagtaacgc ttcctcgttt aaaaatccta gaagttcttc
     8041 aggagccgga caaccatcac gtcagtgcgg aagatttata caaacgtctg atcgatatgg
     8101 gtgaagaaat tggtctggct acggtatatc gcgtactgaa ccagtttgac gacgctggta
     8161 tcgtcacccg ccacaatttt gaaggcggta aatccgtatt tgaactgaca cagcaacatc
     8221 accacgatca cctgatctgc ctcgactgcg gcaaggttat cgaatttagt gatgattcca
     8281 tcgaagcgcg tcagcgtgaa attgccgcaa aacatggcat tcgcctgact aaccacagtc
     8341 tctatcttta cggtcactgt gccgaaggcg attgccgcga agatgaacat gcgcacgaag
     8401 gcaaataagc cagcctgaat gagaaaaagc caacctgcgg gttggctttt ttatgcaagg
     8461 gaaataagaa aagcgagagt tacagctctc acttatttgt tattactgcg aatttcctgc
     8521 caaactttat cacaatcggc tttcaccgcc tgatcatttc cggtttgcgt tgcacgcaga
     8581 cactgctgat aatccagtac gcggacactt tcctggtcgg caaaatgctg atgttgtttc
     8641 tctttcttca gcacgtttaa cacgctctgg caggcttcaa ttttttctgg cgaaccttgt
     8701 gcggtgttga tacaggcgct gtatgcctct ttcagacgac tgtcttcctg tggcgcggtt
     8761 gattgcgcac aagctaccag ccccgatgcc attatggcga tgagaatcag ttttttcata
     8821 gcaaattcct caaacgtgcg gcaggcacaa aacctgccgc gtcgggcatc agaagatggt
     8881 gaatggtgcg attaccataa atttcacgtc acgctcatcc tggaagatgt tgccgtaacc
     8941 accgccccag cttgggatgt cggagtggtt gtcgtattcg gtgaagtgca gtttgaacat
     9001 cgtgcctttg gcgcgaccgt cctgaatggt gtagaccgca tccaggctgt atgcagactc
     9061 ttcaatagtc cggtttttgt cgtagtacgc atccggattg ctctgctagg tcgcaggttt
     9121 agcatcccat gcgtaaacgt aggaagcgcc gatggcgaag cctggaagat tccagttttt
     9181 caggtcatac atcgcaccga agaagaccgc tttttcgccg ttagcgttga agtcggaacg
     9241 gttatcccac cagatatcca ggcggccgtt ggaggaagcg taggttggag tcatacgttg
     9301 caggaagtat ccctgctgac cgtcagcctt aacccaggtg ccttcgaggc gcaaatccac
     9361 tacgtcagcc gcccggtaac caaaggtcaa cgcttgcagc caggcggtgc cgtcataaag
     9421 gtcgttgacg ctgcgatcgt caactttatc gcgcgtacca tagaactggt agctggtggt
     9481 taacgggcta ccggcgatat caaatttgta gctggtggtt aacgggctac cggcgatatc
     9541 aaatttgtag ctggctttgg caaaatattg atcgatatac ccttccgcct gaccaaatgc
     9601 cgcttccagt acgaagttat ttttgaagtc gtatttcgcc ccaagggagt gcagataatc
     9661 aactttggtg gttttatcgt tctgataaaa ctcatccatt tccagatgcc acggcgcttt
     9721 gtattcgttg gtccacatgt aggagaaact caacgcacca gcatcgccgt aatcaaaatt
     9781 cgccccggct tccgccccct gataagtacc tggcataaag ctccagtgcg gcgctaacag
     9841 cgtttgacca gttggctgaa tgtaacctgc cctcgcccaa accggaccgt atttaaattt
     9901 ggccgcagct ttatacaggc ttataccgct tttgtcgccg gaccagtctt cgtcataggc
     9961 tttattactt tttgaaaacg cgatttcgtt cgggtgggag ctgtcgccgt tttccgccat
    10021 ttcaatcgcc gtaaacgcag caatatcaag gccgaacata tcagcagcat aaccggactg
    10081 aaaatcgagg ttggcattcc aggtggagtg agaaaggttg gttttgtatt tgtcgccgtc
    10141 ggtaacatct ttacggtcgc gttcacgctg ccagtaatat ataccgccgg ttaaggttga
    10201 atcgt
//
LOCUS       DAKISA010000139         9998 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.140, whole genome shotgun sequence.
ACCESSION   DAKISA010000139 DAKISA010000000
VERSION     DAKISA010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9998
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.140"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            1..611
                     /locus_tag="OVW45_003796"
                     /pseudo
     CDS             1..611
                     /locus_tag="OVW45_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            608..1222
                     /locus_tag="OVW45_003797"
     CDS             608..1222
                     /locus_tag="OVW45_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HCV5528136.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1279..2736)
                     /gene="pepD"
                     /locus_tag="OVW45_003798"
     CDS             complement(1279..2736)
                     /gene="pepD"
                     /locus_tag="OVW45_003798"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCV5528137.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2998..3456
                     /gene="gpt"
                     /locus_tag="OVW45_003799"
     CDS             2998..3456
                     /gene="gpt"
                     /locus_tag="OVW45_003799"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCV5528138.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3548..4792
                     /gene="frsA"
                     /locus_tag="OVW45_003800"
     CDS             3548..4792
                     /gene="frsA"
                     /locus_tag="OVW45_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCV5528139.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4850..5251
                     /gene="crl"
                     /locus_tag="OVW45_003801"
     CDS             4850..5251
                     /gene="crl"
                     /locus_tag="OVW45_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCV5528140.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5290..6345)
                     /gene="phoE"
                     /locus_tag="OVW45_003802"
     CDS             complement(5290..6345)
                     /gene="phoE"
                     /locus_tag="OVW45_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCV5528141.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            6633..7736
                     /gene="proB"
                     /locus_tag="OVW45_003803"
     CDS             6633..7736
                     /gene="proB"
                     /locus_tag="OVW45_003803"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCV5528142.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7748..9001
                     /gene="proA"
                     /locus_tag="OVW45_003804"
     CDS             7748..9001
                     /gene="proA"
                     /locus_tag="OVW45_003804"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCV5528143.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9116..9191
                     /locus_tag="OVW45_003805"
     tRNA            9116..9191
                     /locus_tag="OVW45_003805"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9149..9151,aa:Thr,seq:cgt)
BASE COUNT         2497 a         2400 c         2595 g         2506 t
ORIGIN      
        1 ctattttaca gcggcatatt gcctcgtttt cgcatcatgg tttgcctgaa ggcagtgacg
       61 acgcgctacg gtatattgcg gaacatgacg atgcgctggc gtttgcgcgt attaatcgcc
      121 agctgatctc tttacgcata atgcaacagg ttaaggccac cggaagtccg gttctggatg
      181 tggcgcataa ctttgttagc gcgtgtcaaa tcggcgatca acagggctgg ttgcatcgta
      241 aaggtgccac gccggatgac aacggtctgg tgattattcc cggttcactc ggtgattact
      301 cctagcttgt acagcctgtt gcgaacgaaa aaacgctcca ctcgctggcg catggggctg
      361 ggcgtaaatg ggggcgcacc gagtgtaaag ggcgtctggc agcgaaatac acagcgacgc
      421 agctctcacg tactgaactt ggcagtcggg taatttgtcg cgataaacaa ctcatctttg
      481 aagaagcgcc acaagcctat aaatcggctg aaagcgtggt gcaatgtctg gtgcaggctg
      541 gtttaattat tcctgtcgcg cggctgcgtc cggtgctaac gctcaaaaac agtggaggga
      601 aaaaaggatg atcttgctac aactctcctc agcgcagggg ccggaagaat gttgtctcgc
      661 agtgaaaaag gcgctggagc gattaattaa agaagctgcc cgacaagacg tcgcggtaac
      721 ggtgttggaa acagaaacgg gccgctactc tgacacgctg cgttcagctc tggtttctct
      781 ggatggcgat aacgcatggg cattaagcga aagctggtgc ggcactattc agtggatttg
      841 tccgagtccg tatcggcctc atcatgggcg caaaaactgg tttctgggca ttgggcgttt
      901 taccgctgat gagcaggaac aatcggatgc aatccgttat gagacgttgc gttcgtcggg
      961 gccgggcggt caacatgtca ataaaaccga ctcggcggta cgcgccacgc atttggcatc
     1021 cggtattagc gtgaaggttc agtcagagcg tagtcagcat gctaacaagc ggctggcacg
     1081 attgctgatt gcctggaagc tggagcagca gcaacaggaa aatagcgcgg tgctgaaatc
     1141 gcagcggcga atgttccatc accagattga acgtggcaac ccgagacgga catttacagg
     1201 gatggctttt atcgaaggat aatgaaggat gaaactgccg gaaggcgatt aaacgccatc
     1261 cggcagcaaa tcaaataatt acttcgccgg aatttctttc agcagttcag tcagcagtgt
     1321 ccagtaatga cctacgcttt cgatgtgaac ttgctcatcc ggagagtgtg gaccggtgat
     1381 agttggcccg atagaaacca tgtccatttc cggatacggt tttttgaaca gaccacattc
     1441 caggcccgcg tggataatct ggatgttcgg cgttttgttg aacagacgct gataggtttc
     1501 acgtaccaga tgcatcaccg gagaattagc gtccggctgc cagccaggat atgcgccttt
     1561 cgcttcggtt ttcgcgccag ccagtttacc cagcgaatcc agcatgctca ccacgtagtc
     1621 tttaccgctg tcgatcagag aacggatcag acagtgaatt tctacgttat tatcagtcat
     1681 ggtcaccaca ccgacgttca gggaggtttc gaccacacct tttgccacgt ctgagttgcg
     1741 gatcacaccg ttcggggtgg cgttcagcag acgaataaag gtatcgcgag atttcgcaat
     1801 cagggcagct ttatcgttcg ctacagagtc cagcaacaag gccagatttt tctctttctc
     1861 tgccagctcg tttttcagga tctcctgata ggtattcacc agagatttca ggacgtcgac
     1921 tttatcagct gcgacagcaa tggtcgcaaa ggcttcacgc gggatggcgt tacgcagtgt
     1981 gccgccgttg aaatcgataa ggcgcagatc cagttcttct gcatgacccg ctaggaagcg
     2041 caccagcagt ttgttggcat tacccaggcc aacgtggatt tccccgccgg agtgaccccc
     2101 tttcagacct tttaaggtta acttgaaggt ttcaaaacca gctggaaccg cttcacgatc
     2161 taaatgcagg ttggaggtga agtcgatacc ccccgcacaa cccatgtaga tttcaccttc
     2221 ttcttcggag tcggtgttaa tcagaatatc agcctgcaac cagttgctct gtaagccgaa
     2281 cgcaccgtcc ataccggctt cttcggtcat ggtcagcagc acttccagcg ggccgtgaac
     2341 cacgttttcg tcagccagaa ccgccagcgc agacgccata ccaatgccgt tatctgcacc
     2401 cagcgtggtg ccgcgcgctt taacccattc gccatcaata taaggctgga taggatcttt
     2461 cgtgaagtca tgcacggtgt cgttattttt ctgcggcacc atatcgaggt gggcctgtaa
     2521 gacgaccgat ttacgatttt ccatacctgc ggtagcaggt ttacgaatca ggatattacc
     2581 tacctgatcg cgttcgacat ggaaaccttt ctcttttgcc caaccaacaa tgtattcagc
     2641 gagttgctct tcatgatagg acgggtgagg aatagaacag attttggcaa aaatatccca
     2701 cagcggctgt ggagataatt gagacagttc agacacgtta agtctccttg tcgatcaccc
     2761 gcaaaacagt attgcaggtc acagggttag cagaaaatgt tgtcaacaca agacaggctt
     2821 gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag
     2881 acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac
     2941 ctattttccc cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg
     3001 agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc
     3061 cgactgatgc cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctggta
     3121 ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt ttgtatttcc
     3181 agctacgatc acgacaacca gcgcgagctt aaagtgctaa aacgcgcaga aggcgatggc
     3241 gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgcggt tgcgattcgt
     3301 gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg
     3361 gttgatgact atgttgttga tatcccgcaa gatacctgga ttgaacagcc gtgggatatg
     3421 ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct ggcactgccg
     3481 ggcgttgttc tttttaactt caggcgggtt acaatagttt ccagtaagta ttctggaggc
     3541 tgcatccatg acacaggcaa acctgagcga aaccctgttc aaaccccgct ttaaacatcc
     3601 tgaaacctcg acgctagtcc gccgctttaa tcacggcgca caaccgcctg tgcagtcggc
     3661 ccttgatggt aaaaccatcc ctcactggta tcgcatgatt aaccgtctga tgtggatctg
     3721 gcgcggcatt gacccacgtg aaatcctcga cgtccaggcg cgtattgtga tgagcgatgc
     3781 cgaacgtacc gacgatgatt tatacgatac ggtgattggc taccgtggtg gcaactggat
     3841 ttatgagtgg gccatccagg cgatggtgtg gcaacaaaaa gcctgtgcgg aagaagatcc
     3901 gcaactcagt ggtcgtcact ggctgcatgc ggctacgttg tacaacattg ctgcctatcc
     3961 tcatctgaaa ggagatgacc tggccgagca agcgcaggct ttgtcaaacc gtgcctatga
     4021 agaagccgcc cagcgtctgc cgggcacgat gcggcagatg gagtttaccg tacccggcgg
     4081 tgcgcccatc accggctttt tgcatatgcc gaaaggcgat ggcccgttcc cgacagtatt
     4141 aatgtgtggt ggtctggatg cgatgcagac ggactattac agcctgtatg aacgttattt
     4201 tgcgccgcgc ggcattgcga tgctgactat tgatatgccg tcggtgggct tttcatcaaa
     4261 atggaagctc acccaggact ccagcctgtt gcatcagcac gttttaaagg ctctgcctaa
     4321 cgtaccgtgg gtggatcaca ctcgcgtcgc ggcctttggt ttccgtttcg gcgctaacgt
     4381 tgccgtgcgt ctggcatatc ttgaatcgcc gcgtctgaaa gcggttgcct gtcttggtcc
     4441 ggtagttcat accctgttga gtgattttaa gtgccagcaa caggtgccgg aaatgtatct
     4501 tgacgttctg gcgagtcgtt tggggatgca tgatgcttcc gacgaagcgt tacgcgtgga
     4561 gctgaatcgc tattcattaa aagtgcaagg attgctgggg cgtcgctgcc caacgccaat
     4621 gttatcaggc tactggaaga acgatccgtt cagcccggaa gaggactcac gcttaatcac
     4681 ctcatcatct gctgacggta aattattaga gatcccattt aacccggtgt atcggaattt
     4741 tgacaaaggt cttcaggaaa tcaccggctg gatcgaaaaa cgcttgtgtt aaaaatttgc
     4801 taaaatttgc caatttggta aaacagttgc atcacaacag gagattgcaa tgacgttacc
     4861 gagtggacac ccgaagagca gattgatcaa aaaatttacc gcactaggcc cgtatattcg
     4921 tgaaggtaag tgcgaagata atcgattctt tttcgattgt ctggctgtat gcgtcaacgt
     4981 gaaaccggca ccggaagtgc gtgagttctg gggctggtgg atggagcttg aagcgcagga
     5041 atcccgtttt acctacagtt accagtttgg tctgttcgat aaagcaggcg actggaagag
     5101 tgttccggta aaagacactg aagtggttga acgactggag cacaccctac gtgagtttca
     5161 cgagaagctg cgtgaactgc tgacgacgct gaatctgaag ctggaaccgg cggatgattt
     5221 tcgtgatgaa ccggtgaagt taacggcgtg agtgaaatgt gccggatgta tcacatccgg
     5281 caatatccat taaaactgat aggtcatgcc aaccgcgaca atatcatcat tattaatatt
     5341 caatttgtta tcgctatcca gttggttgat tttataatca acaaacgctg acatattttt
     5401 gttgaaataa tacgtagcac cgacgtcgat ataattgacc agatcttcat caccgatacc
     5461 ttcaatatct ttccctttcg ataagacata acccagcgat ggacgcagac caaagtcaaa
     5521 ctggtattga gcgaccgctt caaagttctg tgtcttattg gcaaagccgc cagttattgg
     5581 cgtcattttg cgtgtttcag aatagaaagt tgccagataa atattattgg catcgtattt
     5641 cagacctgtt gcccatgctt ctgcacgctt gcctgtgcca cggctttgca ggttctgctc
     5701 gttggtgcga tctgagttgg tataggcccc actaatggcg aaatcgctgc cgccaaagtc
     5761 atatgtcaat gacgtgccga agccatcgcc gttttgcttt ttaacgtcgc ggttttcgtt
     5821 tttcccttga tattgcaggg ttaagttcag gccatcgata acgccgaaga agtcggtgtt
     5881 ccgatacgtc gccagaccgc tggcgcgttt ggtcataaag ttgtcggtct gcgccgagga
     5941 atcgccacca aattccggga acatatcggt ccaggcttcc acgtcataca acgcgcccag
     6001 gttacgacca taatcgaaag aacccaaatc tttatatttc aacccggcaa aagcgagacg
     6061 cgttttctgc tgtgcagtat cactctccgc tttatttccg gcaaactccg cttcccaccg
     6121 accataacca gtcagttgat cgttaatttg tgtttcacct ttaaaaccaa aacggatata
     6181 actctggtcg ccatctttac tggcgttatc actcatataa tgcatggctt taactttgcc
     6241 atagacatcc agtttattac cgtctttatt atatatttct gcagcctgta cagatgcaga
     6301 tgccacaatg cccatcacca ctaatgccag agtgctcttt ttcattttca ttcctgattt
     6361 taattaacgc gcgaatattc agcgggagag ccccgttgaa aacaggaaag tttttaacct
     6421 gagattgtta aagatatatt acagattaat aatattctta aaatgtggta atttattaaa
     6481 tctgtaataa aagcgtaaac aactgccgct aggcttgctg atcccgcgca acaaaacgcc
     6541 atgctttgct cgcagatggt tggcaaccga cgacagtcct gctaaaacgt tcgtttgata
     6601 tcatttttcc taaaattgaa tggcagagaa tcatgagtga cagccagacg ctggtggtaa
     6661 aactcggcac cagtgtgcta acaggcggat cgcgccgtct gaaccgtgcc catattgttg
     6721 aacttgttcg ccagtgcgcg cagttacatg ccgccgggca tcggattgtt attgtgacgt
     6781 cgggcgcgat cgccgccgga cgtgagcacc tgggttaccc ggaactgcca gcgactatcg
     6841 cctcgaaaca actgctggca gcggtagggc agagtcgact gattcaactg tgggaacagc
     6901 tgttttcgat ttatggcatt cacgtcgggc aaatgctgct gacccgtgct gatatggaag
     6961 accgtgaacg cttcctgaac gcccgcgaca ccctgcgtgc gttgctcgat aacaatatcg
     7021 ttccggtaat caatgagaac gatgctgtcg ctacggcaga gattaaagtc ggcgataacg
     7081 ataacctttc tgcgctggcg gcgattctgg cgggtgccga taaactgttg ctgctgaccg
     7141 atcaaaaagg tttgtatacc gctgacccgc gcagcaatcc gcaggcagaa ctgattaaag
     7201 atgtttacgg cattgatgac gcactgcgcg cgattgccgg tgacagcgtt tcaggcctcg
     7261 gaactggcgg catgagtacc aaattgcagg ccgctgacgt ggcttgccgt gcgggtatcg
     7321 acaccattat tgccgcgggc agcaagccgg gcgttattgg tgatgtgatg gaaggcattt
     7381 ccgtcggtac gctgttccat gcccaggcga ctccgcttga aaaccgtaaa cgctggattt
     7441 tcggtgcgcc gccggcgggt gaaatcacgg tagatgaagg ggcaactgcc gccattctgg
     7501 aacgcggcag ctccctgttg ccgaaaggca ttaaaagcgt gactggcaat ttctcgcgtg
     7561 gtgaagtcat ccgcatttgc aacctcgaag gccgcgatat cgcccacggc gtcagtcgtt
     7621 acaacagcga tgcattacgc cgtattgccg gacaccactc gcaagaaatt gatgcaatac
     7681 tgggatatga atacggcccg gttgccgttc accgtgatga catgattacc cgttaaggag
     7741 ctggccgatg ctggaacaaa tgggcattgc tgcgaagcaa gcctcgtata aattagcgca
     7801 actctccagc cgcgaaaaaa atcgcgtgct ggaaaaaatc gccgatgaac tggaagcaca
     7861 aagcgaaatc atcctcaacg ctaacgccca ggatgttgct gacgcgcgag ccaatggcct
     7921 tagcgaagcg atgcttgacc gtctggcact gacgcccgca cggctgaaag gcattgccga
     7981 cgatgtacgt caggtgtgca acctcgccga tccggtgggg caggtaatcg atggcggcgt
     8041 actggacagc ggcctgcgtc ttgagcgtcg tcgcgtaccg ctgggggtta ttggcgtgat
     8101 ttatgaagcg cgcccgaacg tgacggttga tgtcgcttcg ctgtgcctga aaaccggtaa
     8161 tgcggtgatc ctgcgcggtg gcaaagaaac gtgtcgcact aacgctgcaa cggtggcggt
     8221 gattcaggac gccctgaaat cctgcggctt accggcgggt gccgtgcagg cgattgataa
     8281 tcctgaccgt gcgctggtca gtgaaatgct gcgtatggat aaatacatcg acatgctgat
     8341 cccgcgtggt ggcgctggtt tgcataaact gtgccgtgaa cagtcgacaa tcccggtgat
     8401 cacaggtggt ataggcgtat gccatattta cgttgatgaa agtgtagaga tcgctgaagc
     8461 attaaaagtg atcgtcaacg cgaaaactca gcgtccgagc acatgtaata cggttgaaac
     8521 gttgctggtg aataaaaaca tcgccgatag cttcctgccc gcattaagca aacaaatggc
     8581 ggaaagcggc gtgacattac acgcagatgc agctgcactg gcgcagttgc aggcaggccc
     8641 tgcgaaggtg gttgctgtta aagccgaaga gtatgacgat gagtttctgt cattagattt
     8701 gaacgtcaaa atcgtcagcg atcttgacga tgccatcgcc catattcgtg aacacggcac
     8761 acaacactcc gatgcgatcc tgacccgcga tatgcgcaac gcccagcgtt ttgttaacga
     8821 agtggattcg tccgctgttt acgttaacgc ctctacgcgt tttaccgacg gcggccagtt
     8881 tggtctgggt gcggaagtgg cggtaagcac acaaaaactc cacgcgcgtg gcccaatggg
     8941 gctggaagca ctgaccactt acaagtggat cggcattggt gattacacca ttcgtgcgta
     9001 aataaaaccg ggtgatgcaa aagtagccat ttgattcaca aggccattga cgcatcgccc
     9061 ggttagtttt aaccttgtcc accgtgattc acgttcgtga acatgtcctt tcagggccga
     9121 tatagctcag ttggtagagc agcgcattcg taatgcgaag gtcgtaggtt cgactcctat
     9181 tatcggcacc attaagaaaa ttaataactt agcttaactt ctctaagaaa ttgtatcttc
     9241 tgttatcact tacaaacgcc tatacgttgt tatgggcttt ggtagtgtaa cacgtatggg
     9301 cgagtagttc ccaggctcaa cctcgtaaca agttcctttg aagctggaag acgtgccacc
     9361 gtctctaaac cttctaccaa aactcttagc aagacatata acgccttgaa ttgatgtatc
     9421 aggggaaaag tgatggattt tttacgtagg agcacgaaag ggcttctatt gacttttgta
     9481 tgctatgaaa tataattaaa gttttttgtt tttattgtgt gatgtggtgg gttacacagg
     9541 ggaatagatt aaatggtgtt ctgtttatac aggtttattt gttagatcaa caatttggca
     9601 tttctaaaca gatgtttgaa cgttgccaaa tgtcttgaca acacaagaaa agggcttgct
     9661 atgccaggca ttacacgaaa taaggctata aaaacaaaat aaattttaaa atttgcacaa
     9721 acgagaaact cgttcagaaa caagcagaac aatttttagc caacaatttc tgatggtgct
     9781 ctgtaagccg cattacacaa ggctttgcga aagtgatgta aaagtgaaga aagtaagaat
     9841 tgaactttta agctactttt cattggattt tttagtaggg aattacacag ggaatagaga
     9901 gcattattct atgatttaca cagggaatat agggggatta tggcacacac atagaaagac
     9961 attacacaga gtgctatgtg acaaatggat gtaacgag
//
LOCUS       DAKISA010000140         9538 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.141, whole genome shotgun sequence.
ACCESSION   DAKISA010000140 DAKISA010000000
VERSION     DAKISA010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9538
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.141"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(403..609)
                     /locus_tag="OVW45_003806"
     CDS             complement(403..609)
                     /locus_tag="OVW45_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCV5528144.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            922..1011
                     /gene="kdpF"
                     /locus_tag="OVW45_003807"
     CDS             922..1011
                     /gene="kdpF"
                     /locus_tag="OVW45_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCV5528145.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1011..2684
                     /gene="kdpA"
                     /locus_tag="OVW45_003808"
     CDS             1011..2684
                     /gene="kdpA"
                     /locus_tag="OVW45_003808"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCV5528146.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2707..4755
                     /gene="kdpB"
                     /locus_tag="OVW45_003809"
     CDS             2707..4755
                     /gene="kdpB"
                     /locus_tag="OVW45_003809"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HCV5528147.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRILGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHIEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4764..5336
                     /gene="kdpC"
                     /locus_tag="OVW45_003810"
     CDS             4764..5336
                     /gene="kdpC"
                     /locus_tag="OVW45_003810"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCV5528148.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASIPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5329..8013
                     /gene="kdpD"
                     /locus_tag="OVW45_003811"
     CDS             5329..8013
                     /gene="kdpD"
                     /locus_tag="OVW45_003811"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HCV5528149.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPSEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            8010..8687
                     /gene="kdpE"
                     /locus_tag="OVW45_003812"
     CDS             8010..8687
                     /gene="kdpE"
                     /locus_tag="OVW45_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HCV5528150.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8962..9066
                     /locus_tag="OVW45_003813"
     CDS             8962..9066
                     /locus_tag="OVW45_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCV5528151.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9377..>9538
                     /locus_tag="OVW45_003814"
     CDS             9377..>9538
                     /locus_tag="OVW45_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCV5528152.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVNDVE
                     RGKLDEIDAT"
BASE COUNT         2072 a         2437 c         2670 g         2359 t
ORIGIN      
        1 ccgagcaggg gattgaccgg atggccggac gtcactccgc cggggcacac cgaacaggcc
       61 acaccggtgg gggcaccgat gcgcaccccg gctgaaccct ggacaatgct accgccatac
      121 tgcgtcatgt cgccctgacg cgccgccggt tttccgctca tttctcatcc ttgttttatc
      181 gggttatttg tccgctgaca ggcaagaccc tacagattta taagaaagcc ttaagtgaga
      241 tatccgtcag atagaaacaa ttaggaaaat atttatatat atttcatgaa tatatgtttc
      301 gattggtata acggtgtcta aatattcctc tgaacatctt cctgatacaa aaaaccgcca
      361 gcgtttccgc tggcggtttt caatgtattc agaactcagc aatcagtaaa aatcaccggt
      421 tgccttttcg gcctgatcca tccacaccgg tttatcgctg gttttcgacc agacgcggtg
      481 caggtagctg taaaagcggg cgcggtcttt ccagaaaagc atgaaaggca gcgccagaac
      541 gcccgctgca accgcatatg tacggcgtaa aaagataagc catgcaggat attctctgta
      601 gagttccata cttttctccc ccttttgtat ctacccggtg aatagcaccg gaaaaatgaa
      661 tttgtttatc tgatgaaaat agtaccgcct tttgtgtaat tttactactc atccgaccac
      721 ttatttttgc ttattgatgg tttatttaca ttcactctgt aattaagtta cacaaaagtt
      781 aaattaatac taaacattag ttaaatcatg gcttttgcca tttttatact ttttttacac
      841 cccgcccgca gatttttgcg aaatctttgc agccagaatt ctactcttcc ggtatcactt
      901 ttaggccact ggaggtgcac tgtgagtgca ggcgtgatag ccggcgtgtt gctggtgttt
      961 ttattactgg gttatttggt ttatgccctg atcaatgcgg aggcgttctg atggctgcgc
     1021 aagggttctt actgattgcc acgtttttac tggtattaat ggtgctggcg cgtcctttag
     1081 gcagcgggct ggcgcggctg attaatgaca ttcctcttcc aggtacagcg ggcgttgaac
     1141 gtatactctt tcgtttacct ggcgtctctg accatgagat gaactggaag caatatcttt
     1201 gtgccattct cggcctgaac atgctggggc tggcggtgct gttttttatg ttgctcggtc
     1261 agcactatct gccgcttaat ccacagcagt tgccggggct gtcgtgggat ctggcgctga
     1321 ataccgccgt cagctttgtc acgaatacca actggcaatc ttatagcggt gaaaccacgt
     1381 tgagctattt cagccagatg gcaggcttaa cggtacaaaa ctttctttct gccgccagcg
     1441 ggattgcggt gatttttgcc ttcatccgtg cgtttactcg ccagagcatg agcacactcg
     1501 ggaatgcctg ggtcgatctg ctacgcatca cgttatgggt gctagtccct gtggcgttgt
     1561 tgatcgcact gttttttatt caacaaggtg cgctgcaaaa ctttctgccc tatcaggcag
     1621 tgaataccgt tgaaggagcg caacagctgt tacccatggg accagtagct tctcaggaag
     1681 cgatcaagat gctcggtact aacggcggtg gcttctttaa tgccaactcg tcgcatccgt
     1741 ttgaaaaccc aaccgcactg accaacttcg tgcagatgct ggcgatcttc ttgatcccaa
     1801 cagcgctgtg ctttgccttt ggtgaagtga cgggcgatcg ccgccagggg cgcatgttgc
     1861 tgtgggcgat gtcagtgatt tttgtcatct gcgtaggcgt ggttatgtgg gcagaagttc
     1921 agggtaatcc tcatctgctg gcgctcggcg cagacagcag catcaatatg gaaggtaaag
     1981 agagccgttt cggcgtgctg gtcagtagcc tgtttgcggt cgtgactacg gcggcgtcct
     2041 gtggcgcggt gattgcgatg catgattcgt ttaccgctct cggcggcatg gtgccgatgt
     2101 ggctaatgca aattggtgaa gtggtgttcg gcggtgtcgg ttctggtctt tacggcatga
     2161 tgctgtttgt cctgctggcg gtgtttattg ccgggctgat gattggtcgt acaccggaat
     2221 atctgggtaa aaaaatcgac gtacgcgaga tgaaactgac tgcactggcg attctggtta
     2281 ctccaacact ggtactgatg ggcgcggcgt tggcgatgat gaccgacgcc ggacgtagcg
     2341 ccatgcttaa ccctggcccg catggtttta gcgaagtgct gtacgccgtg tcgtccgccg
     2401 ctaacaacaa cggcagcgcc tttgccggat taagcgccaa ctctccgttc tggaactgtt
     2461 tactggcgtt ctgcatgttt gtcggtcgct tcggggtgat tatcccggtg atggcaattg
     2521 ccggttcgct ggtgagtaaa aagagccaac ccgccagctc cggcacgctg ccaacgcacg
     2581 gcccgctgtt tgttggcctg ttaatcggca ccgtgttgct ggttggcgca ctgaccttta
     2641 tccctgccct ggcgcttggt ccggtggcgg aatatctctc ctgatgatat tgagcgagca
     2701 ctgaatatga gtcgtaaaca actggcgcta ttcgaaccaa cacttgtcgt tcaggcgctg
     2761 aaagaagcgg tgaaaaaatt aaacccgcag gcgcaatggc gcaatccggt gatgtttatc
     2821 gtctggatcg gcagtctgct gaccacctgt attagcatcg cgatggcaag cggtgcgatg
     2881 cctggcaatg cgctgtttag cgcggcaatt agcggctggc tgtgggtcac cgtactgttc
     2941 gctaatttcg ccgaggcgct ggcagaaggc cgcagtaaag cgcaggccaa cagtcttaaa
     3001 ggggtgaaaa aaactgcctt tgcccgcaag ctgcgtgagc cgaaatatgg cgctgcggcg
     3061 gacaaagttc ctgccgatca actgcgtaaa ggtgacatcg tgctggtaga agctggcgat
     3121 attatcccct gcgatggtga agtcattgaa ggcggcgcat cggtcgatga aagcgccatc
     3181 accggggaat cggcaccggt gatccgtgaa tccggcggcg attttgcctc cgtcaccggc
     3241 ggcacgcgta ttctttctga ctggctggtg attgagtgta gcgttaaccc cggtgagaca
     3301 tttctggatc ggatgatcgc gatggtggaa ggcgcacagc gacgcaaaac accgaacgag
     3361 attgccctga ctatcctgct gattgctctg accatcgtct ttttactggc aaccgccacg
     3421 ctgtggccgt tttccgcgtg gggcggtaat gccgtgagtg taacggtact ggtggcgctg
     3481 ctggtctgtc tgatcccaac cactattggc ggcctgttgt cagcgatcgg cgtcgccggg
     3541 atgagtcgga tactgggcgc gaatgtgatt gccaccagcg gacgtgcagt tgaagcggca
     3601 ggtgacgttg acgttctgct actggataaa accggcacca tcacactcgg taaccgtcag
     3661 gcgtcggagt ttatccccgc gcagggcgtg gatgaaaaaa cgctggctga cgccgcacaa
     3721 ctggcttcgc tggctgatga aacgccggaa ggtcgcagta ttgtgatcct cgccaagcaa
     3781 cgttttaacc tgcgcgagcg cgatgtgcag tcgctccatg ccacctttgt accgtttact
     3841 gcgcaaagcc ggatgagcgg gatcaacatc gacaaccgca tgatccgtaa aggttctgtc
     3901 gatgccattc gccgccatat tgaggctaac ggcggtcact tccctgccga tgttgatcaa
     3961 aaagtcgatc aggttgcgcg tcagggagcc acgccgctgg tggtggtgga aggttctcgt
     4021 gtgctgggcg ttattgcgct gaaagatatc gtcaaaggcg gtattaaaga gcgcttcgcc
     4081 cagctgcgca aaatgggcat taaaacggtg atgattaccg gcgataaccg tctgactgcc
     4141 gccgcgatag ctgcggaagc gggtgtcgat gattttctcg ccgaagcgac accggaggcc
     4201 aagctggcat tgatccgtca gtatcaggcg gaaggtcgtt tggtagcgat gaccggcgac
     4261 ggcaccaacg atgctccggc gctggcgcag gcagatgtcg cggtggcgat gaactccggc
     4321 acccaggcgg cgaaagaggc gggcaatatg gtggatctcg actctaaccc gaccaagttg
     4381 atcgaggtgg tgcacattgg caaacagatg ctgatgaccc gtggctcgct gaccaccttc
     4441 agcattgcca acgatgtggc gaaatacttc gccattattc cggcggcgtt cgcggcaaca
     4501 tatccgcagt taaatgcgct gaacatcatg cgcctgcatt cgcccgactc cgccattctc
     4561 agtgcggtca ttttcaacgc cttgattatc gtctttttga ttcctctggc gttaaaaggc
     4621 gtgagttata aaccgcttac cgcttctgcc atgttgcgcc gtaacttatg gatttacggt
     4681 ctgggtgggc tgctggtgcc gtttattggt atcaaagtca ttgatttact gctgaccgtt
     4741 tgcggtctgg tgtgaggttt accatgagtg gattacgtcc ggcattatcg acatttctct
     4801 ttctgttatt gattactggc ggcgtttacc cgctgctgac caccgcactg gggcaatggt
     4861 ggtttccctg gcaggccaac ggttcgttga tccgtgaagg tgatacggtg cgtggctcgg
     4921 cattaatcgg gcagaatttt accggcaacg gctattttca tggtcgcccg tcagcaacgg
     4981 ctgaaatgcc ctataatcca caggcttctg gcgggagcaa tctggcggtc agtaaccctg
     5041 agctggataa actaatagcc gcacgcgttg ctgcattacg ggccgctaac ccggatgcca
     5101 gcgcgagcat tccggttgaa ctggtgacgg catcggcaag cgggctggac aataatatca
     5161 ccccgcaagc ggcggcctgg cagatcccac gcatcgcgaa agcgcgtaat ctcagcgttg
     5221 aacagctcac gcagctgatc gcaaaataca gccaacaacc gctggtgaaa tatatcggcc
     5281 agccggtcgt caacattgtc gaactcaatc tggcgctgga taagcttgat gaataacgaa
     5341 cccttacgtc ccgaccccga tcgtctgctg gaacaaactg ccgcgccgca tcgggggaag
     5401 ctgaaagttt tcttcggtgc ctgtgcaggc gtcgggaaga cctgggcgat gctggcagaa
     5461 gcccagcgac tgcgggcgca agggctggat attgtggttg gcgtggtaga aacccacggg
     5521 cgaaaagata ccgccgcaat gctggaaggg ctggctgttc tgccgccaaa acgcctggcg
     5581 taccgtgggc ggcacatctg cgagtttgac ctcgatgccg ccctcgcccg ccgtccggcg
     5641 ctgatcttaa tggacgaact ggcgcacagt aatgcgccag gttctcgtca ccctaaacgc
     5701 tggcaggaca tcgaagagct gctggaagca ggcattgatg ttttcactac cgtcaacgtt
     5761 cagcatctgg aaagtctgaa tgatgtggtc agcggcgtca ccggaattca ggtacgggaa
     5821 accgtgcccg atcctttttt cgatgccgcc gacgacgtgg ttctggtgga cttgcccccg
     5881 gacgatctgc gccagcggct gaaagaaggc aaagtctata ttgccgggca ggcggagcgc
     5941 gccattgaac attttttccg caaaggcaat ctgatcgccc tgcgcgaact ggcactgcgc
     6001 cgtactgccg atcgcgttga tgagcaaatg cgcgcctggc gggggcatcc tagcgaagag
     6061 aaagtctggc acacgcgcga cgcgatcctt ttatgcatcg gccataacac cggtagtgag
     6121 aagctggtcc gcgcggcggc gcggctggcg tcacggctgg gtagcgtctg gcacgcggtg
     6181 tatgttgaaa cccctgccct gcatcgctta ccggagaaaa aacgtcgtgc aattctcagc
     6241 gccttacgtc tggcgcagga actgggcgcg gagacggcaa cactttctga tccagcggaa
     6301 gagaaagcgg tagtgcgtta tgcccgtgaa cataatctcg gcaagattat tctcggtcgc
     6361 ccggcctcgc gccgctggtg gcgtcgggaa acgtttgctg accgactggc gcgcatcgcc
     6421 cccgatctcg atcaggtgct ggtcgcgctt gatgaaccac ccgcccgcac gattaacaac
     6481 gcgccggata gccgctcttt taaagacaag tggcgggtac agattcaggg atgcgtggtt
     6541 gccgccgcgt tatgcgccgt tatcacctta attgccatgc agtggctgat ggcgtttgat
     6601 gccgccaacc tggtgatgct gtatctgctt ggcgtggtgg tggtggcgct attttatgga
     6661 cgctggcctt cggtggttgc caccgtcatt aatgtggtga gtttcgatct cttttttatc
     6721 gccccacgcg gcacgctcgc cgtctctgat gtgcaatatc tgctgacctt cgcggtgatg
     6781 ttaaccgtcg ggctggtgat cgggaacctt actgctggcg tgcgttatca ggcgcgggta
     6841 gcccgttacc gcgagcaacg cacacggcac ttatatgaaa tgtcgaaagc tctggcggtg
     6901 ggccgcagtc cgcaggacat cgccgccacc agcgaacaat ttattgcctc cacgtttcat
     6961 gcccgcagtc aggtgttgtt gcccgatgac aacggtaaat tgcagccgtt aacacatccg
     7021 caaggaatga cgccatggga cgatgccatc gcgcagtgga gttttgataa aggcctgcct
     7081 gcgggcgcgg gcaccgacac gttacccggt gtaccgtacc agattttgcc gctaaaaagc
     7141 ggcgagaaaa cctacgggct ggtggtggtg gagccgggga atctgcgcca gttgatgatc
     7201 ccggaacagc agcgcctgct ggagacgttt acgctgttag tcgccaatgc ccttgagcgg
     7261 ctgacgctaa ccgccagcga agaacaggcg cggatggcaa gcgaacgtga acagatccgc
     7321 aacgccctgc tggcagcgct ttcgcatgat ttacgcacgc cgcttacggt gctgtttggt
     7381 caggcagaaa tcttaacgct cgatctggca agcgaaggat caccccacgc ccgccaggcc
     7441 agcgagattc gtcagcatgt gctgaacact acccgactgg tgaataatct actggatatg
     7501 gcgcgaattc agtccggcgg ctttaatttg aagaaagagt ggttaacgct ggaagaagta
     7561 gtcggcagcg cgctgcaaat gctggaaccg ggtttatcgt cgcccatcaa tctttctctg
     7621 ccagaaccgc tgaccttaat ccacgttgac gggccactct ttgaacgggt gctgattaat
     7681 ctgctggaga acgcggtgaa atatgcgggt gcgcaggccg aaattggtat cgatgcccac
     7741 gttgagggcg aaaatctaca actggatgtc tgggataacg gccccggtct tccgccaggc
     7801 caggagcaga cgatatttga taagtttgct cgcgggaata aagagtcggc ggtgccgggt
     7861 gtcgggcttg ggttggcaat ttgtcgggcg attgtggatg tacacggcgg caccattacc
     7921 gcgttcaacc gaccggaagg tggtgcctgt tttcgtgtta cacttcccca gcaaaccgct
     7981 cctgaacttg aagactttca tgaggatatg tgacaaacgt tctgattgtt gaagatgaac
     8041 aggctattcg tcgctttctg cgcacggcgc tggagggcga cgggatgcgc gtctttgagg
     8101 ccgaaactct gcaacgcggc ttgctggaag cggcaacccg taagccagat ttgattattc
     8161 tcgatctcgg cctgcccgat ggtgatggga ttgagtttat ccgcgacctg cgccagtgga
     8221 gcccggtgcc ggtgattgtg ctttccgcac gcagcgaaga gagcgacaag atcgccgcgc
     8281 tggatgccgg agcggatgat tatctgagta agccgtttgg cattggcgaa ttgcaggccc
     8341 gtctgcgcgt cgctttacgc cgccactccg ccaccgccgc gcccgatccg ctggtgaaat
     8401 tttccgatgt taccgtcgat ttagccgccc gcgtgattca ccggggtgag gaagaggtgc
     8461 atctcacacc aattgagttc cgcctgctgg cggtgctgct caacaatgcc ggaaaagtac
     8521 tcacccagcg ccagctcctt aaccaggtgt gggggccaaa cgcggtcgaa cacagtcact
     8581 atttgcgtat ttatatggga catctgcgac aaaaactgga acaggatccg gcccggcctc
     8641 gccacttcat tactgaaacc ggtattggat atcggtttat gctttgaatt gttattcaat
     8701 aaattcgctt aaatagtgat taatttatta ttaaacctgc gccgaatatt attatttggc
     8761 aggtttaatt tcttaacgca aatataaaac acgaaattac aacatttaag taacaaaaca
     8821 gcatctaata tggcgatatt cataattaat gatttattat ctggggtatg atcgtttttt
     8881 gttgatcttc ttcacagatt atagccattt catggataga ataactctac cttcaactga
     8941 cacagcaaga ggtaaaggta aatggaaaat aataaccgct taatgcctca tataaggcgg
     9001 acaacccata tcatgaagtt tgcccatcgt aatagttttg actttcattt ctttaatgcc
     9061 cgctagtctt ctgactaaag ggcaccccaa cgtacaggtc tccctgactt taagcattac
     9121 aggttaatac ctgtattcct cggtgctcat atactgctaa cccttttaaa ctctaaataa
     9181 ttcgagtcgc agcacttgca acttgaggta tgacgagtat agccagttac cgggctggtc
     9241 tgggttattg catctgcaaa aagcaaacta ctgatttatt tatcagcggt ggagctttgc
     9301 tttttttccg gcgtgatcga tttctccttt gagaaattga ggacctgcta ttacctgaaa
     9361 taaagagatg aataaaatgt cagaattaaa aattgccgtt agccgttctt gcccggattg
     9421 tttttccact catcgtgcat gcgtgaatat tgacgaaagt aattatattg acgttgccgc
     9481 cattatttta tcagtcaatg atgttgaacg tggaaaactc gatgaaatag acgctact
//
LOCUS       DAKISA010000141         9403 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.142, whole genome shotgun sequence.
ACCESSION   DAKISA010000141 DAKISA010000000
VERSION     DAKISA010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9403
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.142"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            206..1072
                     /gene="folD"
                     /locus_tag="OVW45_003815"
     CDS             206..1072
                     /gene="folD"
                     /locus_tag="OVW45_003815"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCV5528153.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1074..1286
                     /gene="ybcJ"
                     /locus_tag="OVW45_003816"
     CDS             1074..1286
                     /gene="ybcJ"
                     /locus_tag="OVW45_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCV5528154.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKADGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1394..1915
                     /locus_tag="OVW45_003817"
     CDS             1394..1915
                     /locus_tag="OVW45_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCV5528155.1"
                     /translation="MPTVITHAAVPLCIGLGLSSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSM
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSCYTTPYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(1951..3336)
                     /gene="cysS"
                     /locus_tag="OVW45_003818"
     CDS             complement(1951..3336)
                     /gene="cysS"
                     /locus_tag="OVW45_003818"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCV5528156.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYAADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3510..4004
                     /gene="ppiB"
                     /locus_tag="OVW45_003819"
     CDS             3510..4004
                     /gene="ppiB"
                     /locus_tag="OVW45_003819"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCV5528157.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4007..4729
                     /gene="lpxH"
                     /locus_tag="OVW45_003820"
     CDS             4007..4729
                     /gene="lpxH"
                     /locus_tag="OVW45_003820"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCV5528158.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4847..5356
                     /gene="purE"
                     /locus_tag="OVW45_003821"
     CDS             4847..5356
                     /gene="purE"
                     /locus_tag="OVW45_003821"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCV5528159.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGTA"
     gene            5353..6420
                     /gene="purK"
                     /locus_tag="OVW45_003822"
     CDS             5353..6420
                     /gene="purK"
                     /locus_tag="OVW45_003822"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCV5528160.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYTLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6557..7450)
                     /locus_tag="OVW45_003823"
     CDS             complement(6557..7450)
                     /locus_tag="OVW45_003823"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCV5528161.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7447..8100)
                     /locus_tag="OVW45_003824"
     CDS             complement(7447..8100)
                     /locus_tag="OVW45_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HCV5528162.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFFAYSDNLQLVTTAVSVSYLR
                     YAAQGYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8111..9370)
                     /locus_tag="OVW45_003825"
     CDS             complement(8111..9370)
                     /locus_tag="OVW45_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HCV5528163.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETDITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
BASE COUNT         2092 a         2666 c         2494 g         2151 t
ORIGIN      
        1 ttagacaagg aaataagtca gttccagatt tacaacgcca tcatggacga aaaatgaagc
       61 tttcaatctc agcgacggtg cgccccacct tcgcaagagg tcgcttcacg cgataaatct
      121 gaaacgaaac ctgacagcgc gccccgcttc tgacaaaata ggcgcatccc cttcgaccta
      181 cgtaacagat ggaatcctct ctctgatggc agcaaagatt attgacggta aaacgattgc
      241 gcagcaggtg cgctctgaag ttgctcaaaa agttcaggcg cgtattgcag ccggactgcg
      301 ggcaccagga ctggccgttg tgctggtggg tagtaaccct gcatcgcaaa tttatgtcgc
      361 aagcaaacgc aaggcttgtg aagaagtcgg gttcgtctcc cgctcttatg acctcccgga
      421 aaccaccagc gaagcggagc tgttggagct tatcgatgcg ctgaatgccg acaacaccat
      481 cgatggcatt ctggttcaac tgccgttacc ggcgggtatt gataacgtca aagtgctgga
      541 acgtattcac ccggacaaag acgtagacgg tttccatcct tataacgtcg gtcgcctgtg
      601 ccagcgcgcg ccgcgtctgc gcccctgcac ccctcgcggc atcgtcacgc tgcttgagcg
      661 ttacaacatt gataccttcg gcctcaacgc cgtggtgatt ggcgcatcga atatcgtcgg
      721 tcgtccaatg agcatggaac tgttgctcgc aggttgcacc actaccgtga ctcaccgctt
      781 cactaaaaat ctgcgtcacc acgtagaaaa tgccgatcta ctgatcgttg ccgtcggcaa
      841 gccgggcttt attcctggtg actggatcaa agaaggcgca attgtgattg atgtcggcat
      901 caaccgtctg gaaaatggca aagttgtggg cgacgtcgtg tttgaagacg cggctaaacg
      961 cgcctcatac attacgcctg ttccgggcgg cgttggcccg atgacggttg ccacgctgat
     1021 tgaaaacacg ctacaggcgt gcgttgaata tcatgatcca cagggtgagt aacatggcga
     1081 cattttcttt aggtaaacat ccgcacgttg agctgtgcga cttgctgaaa ctggaaggct
     1141 ggagcgaaag cggcgcgcag gcgaaaatcg cgattgccga aggccaggtg aaagccgacg
     1201 gcgcggttga aacgcgcaaa cgctgcaaaa tcgtcgccgg tcagacagtg agttttgcag
     1261 gtcacagcgt acaggttgtt gcctgatccc tttcccggcc tgtatcaaca ggccggttct
     1321 cttttcagac ttttgccctc tacgccccgt tcatccatgc tgatagcatc gacattttgc
     1381 cgaagcgacc atcatgccaa ccgttattac gcacgccgcc gttcccctat gcattggttt
     1441 agggttgagt tcgaaagtca tccctccgcg tctgttattt gccggaatca tcctggcgat
     1501 gctgcccgac gccgacgtat tgtcgtttaa atttggcgtt gcttacggca atgtttttgg
     1561 tcatcgcggg tttacccatt cgctggtgtt tgcgtttgtt gtcccgctgt tatgtgtgct
     1621 gattggacgc cgatggttca gggctgggct gattcgctgc tggctgtttt taaccgtctc
     1681 gttgctatcg cacagcatgc tggattcggt aaccactggc ggtaagggtg ttggctggct
     1741 gtggccgtgg tcagatgaac gctttttcgc tccctggcag gtgattaaag tcgcgccgtt
     1801 tgcattgtct tgttacacca cgccgtacgg gcatcaggtg attatttctg aattgatgtg
     1861 ggtgtggttg ccggggatgc tactgatggg aatgttgtgg tggcgcagac gataaccgga
     1921 tgcgaaaact cgcatccggc aatagcgcaa ttacttacga cgccaggtgg tcccttgcgg
     1981 gccatcttcc agaacgatcc ccatctcgtt aagacggtca cgcgccgcat ctgccgccgc
     2041 ccagtctttc gctttacggg catccagacg ctgttgaatt aacgcttcaa tctcagccac
     2101 ttcgctgtcg tctgcctgcg cgccgctttg caggaacgct tccggttctt gctccagcag
     2161 gcctaataca gcggaaagtt tacgcaggtg agatgccatt gcattcgccg ctgccatatc
     2221 ttctgctttc agacggttta cttcacgcgc catatcaaac agtacggaat aggcttccgg
     2281 ggtgttgaaa tcgtcgtcca tcgcttcaat aaagcgcgct tcaaacgctt cgccaccggc
     2341 aggagcaacg gttttgtctg tgccgcgcag ggcagtgtag agacgctcca gcgccgcacg
     2401 cgcctgcttc aggttctctt cgctgtagtt cagctggctg cgatagtggc ccgacatcag
     2461 gaagtaacgt acggtttcag catcgtagta tttcagcaca tcgcgcacgg taaagaagtt
     2521 acccagcgat ttggacatct tctcgcggtc aaccatcacc attccggagt gcatccagta
     2581 gttaacatac tgaccatcat gggcacaggt ggactgcgcg atttcattct cgtggtgcgg
     2641 gaacatcagg tcagaaccgc cgccgtggat atcaaagtgg ttacccagct gcttgcagtt
     2701 cattgccgaa cattcaatgt gccagcctgg acggcccgcg ccccacggag acggccagct
     2761 cggttcgccc tctttcgaca tcttccacag aacgaagtcc atcgggttgc gtttgtcgtc
     2821 gaccacgtca acgcgcgcgc ctgcctgcag ctggtcgaga tcctgacgcg acagcacacc
     2881 ataagtcgga tcggtcggga cgtcgaacat cacgtcgccg ttgtccgccg cataagcgtg
     2941 acctttggcg atcagttgtt cagtgagttc aataatttct gcgatatggt gcgtcgcgcg
     3001 cggctccata tccgggcgca gaatgttcaa agcatcaaaa tctttgtgca tttcggcgat
     3061 catgcgatcc accagcgcca caaagctttc gccattttca ttggcacgtt tgatgatttt
     3121 gtcgtcgata tcggtaatgt tgcgcacata cttcagttta tagccgagga aacgcagata
     3181 gcgcgcaacc acgtcaaaag caacaaaggt acgcccgtga ccgatatgac agagatcgta
     3241 aacggtgatt ccacacacat acatgccgac ttccccggcg tgaataggct taaattcctc
     3301 tttttggcgt gtcagagtat tgaagatttt tagcatcgaa gattccgttt agacatgtgt
     3361 gggtaattga gttgcgtata atacccatat ttcccgcgcg aatcagcata cattgcgtga
     3421 tgatccgatc gtgcggttat gctataacac caccctatat atgacccgaa ctgggttgaa
     3481 gcaccaatca aacggaacag gatgcaaaaa tggttacttt ccacaccaat cacggcgata
     3541 ttgtcatcaa aacttttgac gataaagcac ctgaaacagt taaaaacttc ctggactact
     3601 gccgcgaagg tttttacaac aacaccattt tccaccgtgt tatcaacggc tttatgattc
     3661 agggcggcgg ttttgaaccg ggcatgaaac aaaaagccac caaagaaccg atcaaaaacg
     3721 aagccaacaa cggtctgaaa aatacccgtg gtacgctggc aatggcccgt actcaggctc
     3781 cgcactctgc aactgcacag ttcttcatca acgtggttga taacgacttc ctgaacttct
     3841 ctggcgaaag cctgcaaggt tggggctact gcgtgtttgc tgaagtggtt gaaggcatgg
     3901 acgtggtaga caaaatcaaa ggtgttgcaa ccggtcgtag cggtatgcac caggacgtgc
     3961 caaaagaaga cgttatcatt gaaagcgtga ccgttagcga gtaatcgtgg cgacactctt
     4021 tattgcagat cttcatctct gcgtggaaga accggcgatc accgccggtt ttctgcgttt
     4081 tttagcgggg gaagctcgca aggccgacgc gctgtatatt cttggcgatc tgtttgaagc
     4141 gtggattggc gacgacgatc ccaatccact ccatcgccag atggcggcag cgatcaaagc
     4201 ggtgtccgat tctggcgttc cctgttattt cattcatggc aaccgtgatt ttctgctcgg
     4261 caaacgcttt gcccgtgaaa gtggcatgac gttattgccg gaagaaaagg tgctcgaact
     4321 ttatggccgc cgggtgttga ttatgcatgg cgacacgctg tgcaccgatg acgcgggtta
     4381 tcaggctttt cgcgccaaag tccacaaacc ctggctgcaa atgctattcc tcgccctgcc
     4441 gttgtttgtg cgcaaacgca ttgccgcgcg aatgcgagcg aacagtaaag aagccaacag
     4501 cagtaaatcg ctggcgatca tggacgttaa ccaaaacgcg gtagtcagtg cgatggaaaa
     4561 acatcaggtg caatggctga tccacggaca tacccatcgc ccggcggtac atgaacttat
     4621 cgccaaccag caacctgctt ttcgcgtggt actcggtgcc tggcatacgg aaggttcgat
     4681 ggtgaaagtc acggcggatg acgttgagct gattcatttt ccgttttaaa aaacccgcaa
     4741 ctttgctgat ttcacagcca cgcaaccgtt ttccttgctc tctttccgtg ctattctctg
     4801 tgccctctaa agccgagagt tgtgcaccac aggagtttta agacgcatgt cttcccgcaa
     4861 taatccggcg cgtgtcgcca tcgtgatggg gtccaaaagc gactgggcta ccatgcagtt
     4921 cgccgccgaa atcttcgaaa tcctgaatgt cccgcaccac gttgaagtgg tttctgctca
     4981 ccgcaccccc gataaactgt tcagcttcgc cgaaagcgcc gaagagaacg gttatcaggt
     5041 gattattgcg ggcgcaggtg gcgcagcgca cctgccaggc atgattgccg ccaaaacgct
     5101 ggtgccggtg ctgggcgtgc cagtacagag cgccgcactg agcggtgtcg atagcctcta
     5161 ctccatcgta caaatgccgc gcggcattcc ggtgggtacg ctggcgattg gtaaagctgg
     5221 cgcggcaaac gcggcattac tggcagcaca aattctcgcg actcacgata aagagctaca
     5281 ccagcgtctg aatgactggc gcaaagcaca aactgatgaa gtgctggaaa acccggaccc
     5341 gcgaggtacg gcatgaaaca ggtttgcgtc ctcggtaacg ggcagttagg ccgtatgctg
     5401 cgtcaggcag gcgaaccgtt aggcattgct gtctggccag tcgggctgga cgctgaaccg
     5461 gcggcggtgc cttttcaaca aagcgtgatt accgctgaga tcgaacgctg gccggaaacc
     5521 gcattaaccc gcgagctggc gcgccatccg gcctttgtga accgcgatgt gttcccgatt
     5581 attgctgacc gtctgactca gaagcagctt ttcgataagc tccacctgcc gaccgcgccg
     5641 tggcagttac ttgccgatcg cagcgagtgg cctgcggtgt ttgatcgttt aggtgaactg
     5701 gcgattgtta agcgtcgcac tggtggctat gacggtcgcg gtcaatggcg tttacgcgcc
     5761 aatgaaaccg aacagttacc ggcagagtgt tacggcgaat gtattgtcga gcagggcatt
     5821 aacttctctg gtgaagtgtc gctggttggc gcgcgcggct ttgatggcag caccgtgttt
     5881 tatacgctga cgcataacct gcatcaggac ggtattttgc gcaccagcgt cgcttttccg
     5941 caggccaacg cacagcagca ggcgcaagcc gaagagatgc tgtcggcgat tatgcaggag
     6001 ctgggctatg tgggcgtgat ggcgatggag tgttttgtca ccccgcaagg tctgctgatc
     6061 aacgaactgg ctccgcgtgt gcataacagc ggtcactgga cacaaaatgg tgccagcatc
     6121 agccagtttg agctgcatct gcgggcgatt accgatctgc cgttaccgca accagtagtg
     6181 aataatccgt cggtgatgat caatctgatt ggtagcgatg tgaattatga ctggctgaaa
     6241 ttgccgctgg tgcatctgca ctggtacgac aaagaagtcc gtccggggcg taaagtgggg
     6301 catctgaatt tgaccgacag cgacacatcg cgtctgactg cgacgctgga agccttaatc
     6361 ccgctgctgc cgccagagta tgccagcggc gtgatttggg cgcagagtaa gttcagttaa
     6421 gcaggtgctc atttgattgc cggatgcggc gtaaacgcct tatccggctt accgctccgg
     6481 tacccattgt aggcctgata agacgcgtca agcgtcgcat caggcatggt gcaccgattg
     6541 ccggatgctc tcatcactac agcgaaatac aggtccccgc ttcgcccgcc agcgtctctt
     6601 caattcgcga taacgcccca atccacgcgg gtttaccacg gcttctgaca taaccactca
     6661 ccgccgttac cttcggcccc atcgaaccat cggctttggc aaatggcgct aactcatccg
     6721 gtgtggcatg gcgaatggca cgttgctgcg gcgttcccca gttttcatat accgcatcag
     6781 catcggtgag gatcaccagt ccatcggcat taatctgctc ggcgagcaac gcagcggcga
     6841 gatctttatc aatcactgct tcactccctg ccccatcttc cgtcacaggc acacctccgc
     6901 cgccactgca aatcaccaca tgcccctctt tgagcaacaa ctcgatggct tcgctgtcga
     6961 gaattttacg cggttgcgga gacgccacca cccggcgcaa atatttacca tcacgtttca
     7021 tctgccagcc gtaagccgct tccagtgcct cttgttcttc tggctgataa accggaccaa
     7081 taaatttctc tggctgcaaa aacgccggat catcaggcga aacctcaatg cgagtcagca
     7141 ccgtcgtaac aggcggcatc tgcggctgtg cgctcaagct ctgcgccagc atatagccaa
     7201 tcatcccctg gctttccgcc accagcacat ccagcggata cggttctacc tctttccacg
     7261 ccagattctg taacgccagc agccccacct gcggcccgtt gccgtgaaca atcgccagcc
     7321 gataagaacg ggccaggcgt gccagcgcgg gtacagcact ggcgatattg cgatattgat
     7381 tttccgccgt cagcgcctca ccgcgctgga gtaaggcgtt gcccccaaga gctacaacca
     7441 gtgttttcat ggttttcctt gtaataattg ttggccaagc cagaacccca gcagcgtatc
     7501 tgcccctgac gtatgcccca gcgccagcag cgaatcaatc gcaacagcgg tacgtttcgg
     7561 gcaactcaga gcatggacaa agtgcaggag tggcgaggcg aaatatcctt gcgcggcata
     7621 acgtaaataa ctgacgctca ccgctgtggt aacgagttga agattgtcgg aataggcaaa
     7681 aaactggcgt aactcagcag gcagcggatc gcctgccgcc aacgccagtg gcccgaaaag
     7741 cccggtttcc tccgcgcgtt gcatccatgc agctgcaagc ggttgaggat gcgcaggcgg
     7801 cgtaatacgc agcaaacaat aacgctgtgg ctgtttaacc gtgaaacgcc cgaggcgaat
     7861 cccttgagcc acaacctgtg gtcgttcatt gccgcataat ccaccgcata acgcatcgaa
     7921 ttgcgcgcgg cgaagcaccc atcctccggg gccgaaacca ctaccctgac gatgcagcgt
     7981 taacagttcg ccgctttccg tcatcaggtt aatcgcccgt cgccacactc ccgctaaccg
     8041 ccaggactgg cgataattcg gtgcactacg gctggcaagc agaggatgga tgatcgtcat
     8101 tgcgcgttcc tcaaccaatc cccatgcttt cagccagtgc ttccagcgcc tgttcaaaac
     8161 acgccagcgg tgcccgcaca gtgcctgcgc caatctgccc gatccccgcc tctttatggg
     8221 cgataccggt attgatgagt ggcgtaatgt cggtttctac cacgcgacga atgtccagtc
     8281 ccaggcacgc gccctgaaaa tcccagcctg ggatctgcaa ctgcatattg cgctcaaggt
     8341 aaatttccgc catctcttca gataccgctc tggccgcttc catgccaccc gcaccgacaa
     8401 agcgcgttac gccaggcgct gcgatcattg ccgcacctcc gataccaaag gtttcggtaa
     8461 tcgcgctatc gcccatatcc gggttcgcct gctcctgcga gaagccggtg aaaaacagac
     8521 cttgcggagt gtttacaggc gcagtaaacc agcgttcgcc cagcccgctt acccgaatcc
     8581 cgaacatatt gccgttgcgg gtcattgccg tgacgatgct gcctgcgcgg atcatcgcgc
     8641 cagcatccat cgccgccttg cagtaagcca tcgcgaggtt gaggaagaac tgatcggtca
     8701 cgctgaggaa atccatcact tcggcgatgt gctgtttatc atgatcgagg cgagcaattt
     8761 gtggggccag cgcacgcatt aacagtgcag aggaagcaat attgcgttga tggaactcat
     8821 cgcccatcgt aatgccctgc gccatcatcg ccgtgagatc gataccgcgc tccatgcgcc
     8881 ccagcgccgc gcttaatact ggcattaaca catcgcgcat ccagcgatgg cgagtcagga
     8941 catcttcgcc gtaagcgcca aaacgcatca ctttgccgat accttcgttg aggttgcagt
     9001 acgcccggtt gccgtcggtc acgttctcga ccaccagcat cggcatactg gcagaagtaa
     9061 taccgcccat tggaccgacg gcattcacat ggtgacaagg aatgaagttc acttccccct
     9121 gctccagtat tgccagcgcc tgcgcttcat ctttcgccca gccttcgaac agacatgcgc
     9181 ccacgcacgc ccctttcatg ggtccggtca tctcctgcca gcgcattggc ggcccggcgt
     9241 gaagcagtgt tttgccctcg tttagttcgc tgataagtga agaagccggt tgcacatcca
     9301 gccagtgtgg acgagcgcga cgaatttgtt cgataaccgc agcattggct tgcgccactg
     9361 atgtaaacat gggaacccct tattgcaaac gttctaataa acg
//
LOCUS       DAKISA010000142         9230 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.143, whole genome shotgun sequence.
ACCESSION   DAKISA010000142 DAKISA010000000
VERSION     DAKISA010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9230
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.143"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..1348
                     /gene="tnaA"
                     /locus_tag="OVW45_003826"
     CDS             <1..1348
                     /gene="tnaA"
                     /locus_tag="OVW45_003826"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCV5528164.1"
                     /translation="RAYREEAIIKSGMNPFLLDSEDVFIDLLTDSGTGAVTQSMQAAM
                     MRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQGRGAEQIYIPVLIKKREQEKGLDR
                     SKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFDTGVRYDFKGNFDLEGLERGIEEV
                     GPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAKKYDIPVVMDSARFAENAYFIKQR
                     EAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPMGGLLCMKDDSFFDVYTECRTLCV
                     VQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYRIAQVQYLVDGLEEIGVVCQQAGG
                     HAAFVDAGKLLPHIPADQFPAQALACELYKVAGIRAVEIGSFLLGRDPKTGKQLPCPA
                     ELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIKGLTFTYEPKVLRHFTAKLKEV"
     gene            1439..2686
                     /gene="tnaB"
                     /locus_tag="OVW45_003827"
     CDS             1439..2686
                     /gene="tnaB"
                     /locus_tag="OVW45_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HCV5528165.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLTLVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            2818..3993
                     /gene="mdtL"
                     /locus_tag="OVW45_003828"
     CDS             2818..3993
                     /gene="mdtL"
                     /locus_tag="OVW45_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCV5528166.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            3968..4927
                     /gene="yidZ"
                     /locus_tag="OVW45_003829"
     CDS             3968..4927
                     /gene="yidZ"
                     /locus_tag="OVW45_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCV5528167.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            5084..5833
                     /locus_tag="OVW45_003830"
     CDS             5084..5833
                     /locus_tag="OVW45_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCV5528168.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            5855..6421
                     /gene="yieF"
                     /locus_tag="OVW45_003831"
                     /gene_synonym="chrR"
     CDS             5855..6421
                     /gene="yieF"
                     /locus_tag="OVW45_003831"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCV5528169.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDRQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6475..7812)
                     /gene="adeP"
                     /locus_tag="OVW45_003832"
     CDS             complement(6475..7812)
                     /gene="adeP"
                     /locus_tag="OVW45_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCV5528170.1"
                     /translation="MSHQHTTQTSGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            7978..8643
                     /gene="yieH"
                     /locus_tag="OVW45_003833"
     CDS             7978..8643
                     /gene="yieH"
                     /locus_tag="OVW45_003833"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCV5528171.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTESEHVYRAEVARLFDSELEAIEGAEALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            8710..9183
                     /locus_tag="OVW45_003834"
     CDS             8710..9183
                     /locus_tag="OVW45_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HCV5528172.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         2247 a         2298 c         2321 g         2364 t
ORIGIN      
        1 tcgcgcttat cgtgaagaag caattattaa atccggtatg aacccgttcc tgctggatag
       61 cgaagatgtg tttatcgatt tactgaccga cagcggcacc ggggcggtaa cccaaagtat
      121 gcaggcagcg atgatgcgcg gcgacgaagc ctacagcggc agccgcagct actatgcgtt
      181 agccgagtca gtgaaaaata tctttggtta tcaatacact attccgactc accagggccg
      241 tggcgcagag caaatctata ttccggtact gattaaaaaa cgcgagcagg aaaaaggcct
      301 ggatcgcagc aaaatggtgg cattctctaa ctatttcttt gataccacgc agggccatag
      361 ccagattaac ggctgtaccg tgcgtaacgt ctatatcaaa gaagccttcg atacgggcgt
      421 gcgttacgac tttaaaggca actttgacct cgaaggatta gaacgcggta ttgaagaagt
      481 tggcccgaat aacgtgccgt atatcgttgc aaccatcacc agtaactctg caggtggtca
      541 gccggtttca ctggcaaact taaaagcgat gtacagcatc gcgaagaaat acgatattcc
      601 ggtggtaatg gactccgcac gctttgctga aaacgcctat ttcatcaagc agcgtgaagc
      661 agaatacaaa gactggacca tcgagcagat cacccgcgaa acctacaaat atgccgatat
      721 gctggcgatg tccgccaaga aagatgcgat ggtgccgatg ggcggcttgc tgtgcatgaa
      781 agacgacagc ttctttgatg tgtacaccga gtgcagaacc ctttgcgtgg tgcaggaagg
      841 cttcccgaca tatggcggcc tggaaggcgg cgcgatggag cgtctggcgg taggtctgta
      901 tgacggcatg aatctcgact ggctggctta tcgtatcgcg caggtacagt atctggtcga
      961 tggtctggaa gagattggcg ttgtctgcca gcaggcgggc ggtcacgcgg cattcgttga
     1021 tgccggtaaa ctgctgccgc atatcccggc agaccagttc ccggcacagg cgctggcgtg
     1081 cgagctgtat aaagtcgccg gtatccgtgc ggtagaaatt ggctctttcc tgttaggccg
     1141 cgatccgaaa accggtaaac aactgccatg cccggctgaa ctgctgcgtt taaccattcc
     1201 gcgcgcaaca tatactcaaa cacatatgga cttcattatt gaagccttta aacatgtgaa
     1261 agagaacgcg gcgaatatta aaggattaac ctttacctac gaaccaaaag tattgcgtca
     1321 cttcaccgca aaactgaaag aagtttaatt aatactacag agtggctata aggatgttag
     1381 ccactctctt accctacatc ctcaataaca aaaatagcct tcctctaaag gtggcatcat
     1441 gactgatcaa gctgaaaaaa agcactctgc attttggggt gttatggtta tagcaggtac
     1501 agtaattggt ggaggtatgt ttgctttacc tgttgatctt gccggtgcct ggtttttctg
     1561 gggtgccttt atccttatca ttgcctggtt ttcaatgctt cattccgggt tattgttatt
     1621 agaagcaaat ttaaattatc ctgtcggctc cagttttaac accatcacca aagatttaat
     1681 cggtaacacc tggaacatta tcagcggtat taccgttgcc ttcgttctct atatcctcac
     1741 ttatgcctat atctctgcta atggtgcgat cattagtgaa acgatatcaa tgaatttggg
     1801 ctatcacgct aatccacgta ttgtcgggat ctgcacagcc attttcgttg ccagcgtatt
     1861 gtggataagt tcgttagccg ccagtcgcat tacctcattg ttcctcgggc tgaagattat
     1921 ctcctttgtg atcgtgtttg gttctttctt cttccaggtc gattactcca tcctgcgcga
     1981 tgccaccagc accactgcgg gaacgtctta cttcccgtat atctttatgg ctttgccggt
     2041 gtgtctggcg tcatttggtt tccacggcaa tattcccagc ctgattattt gctatggaaa
     2101 acgcaaagat aagttaatca aaagcgtggt atttggttcg ctgctgacgc tggtgattta
     2161 tctcttctgg ctctattgca cgatggggaa tattccgcgc gaaagcttta aggcgataat
     2221 ctcctcaggc ggcaacgttg attcgctggt gaaatcgttc ctcggcacca aacagcacgg
     2281 cattatcgag ttttgcctgc tggtgttctc taacttagct gttgccagtt cgttctttgg
     2341 tgtcacgctg gggttgttcg attatctggc ggacctgttt aagattgata actcccacgg
     2401 cgggcgtttc aaaaccgtgc tgttaacctt cctgccacct gcgttgttgt atctgatctt
     2461 cccgaacggc tttatttacg ggatcggcgg tgccgggctg tgcgccacca tctgggcggt
     2521 cattattccc gcagtgcttg caatcaaagc tcgcaagaag tttcccaatc agatgttcac
     2581 ggtctggggc ggcaatctta ttccggcgat tgtcattctc tttggtataa ccgtgatttt
     2641 gtgctggttc ggcaacgtct ttaacgtgtt acctaaattt ggctaaatcc ttcaagaagc
     2701 cagccattcg ctggcttctt gcctctcagg aaatcactta tgtccaaatg gcaactcgcc
     2761 tgatcctcct tcaccacgta tgctttgcgt caccttacta tcaggacgct ttagcccatg
     2821 tcccgctttt tgatttgtag ttttgccctg gttttacttt atcccgccgg gattgatatg
     2881 tacctcgttg gtttaccgcg catcgccgcc gatctcaatg ccagcgaagc gcagttgcat
     2941 attgcgttct ccgtatatct ggcggggatg gcagctgcga tgttatttgc cggtaaagtg
     3001 gccgatcgtt cagggagaaa gccggtcgcc atacccggcg cggcgctatt tattattgcc
     3061 tcggtgttct gttcactggc tgaaaccagc acgttatttc ttgcaggccg atttctacag
     3121 gggttgggcg caggctgttg ttacgtagtg gcgttcgcca ttttgcgcga cacgctggat
     3181 gatcgacgtc gggctaaagt gctgtcatta ctcaacggta ttacctgcat cattccggtg
     3241 ttagcgccag tgctcggaca tctgattatg cttaaattcc cgtggcagag tctgttctgg
     3301 gcgatggcaa tgatgggcat cgcggtactg atgttgtctt tgtttatttt aaaagaaacg
     3361 cgcccagcgt cccccgctgc ttcggacaaa ccacgagaaa atagcgagtc gctgcttaat
     3421 cggtttttcc tcagccgtgt tgttatcacc accctcagcg tttcggtgat cctcactttc
     3481 gtcaatacat cgccggtatt gctgatggaa atcatgggtt ttgagcgcgg agaatacgcc
     3541 accattatgg cgttgaccgc tggcgtcagc atgaccgttt cattctccac gccatttgcg
     3601 ctgggaattt ttaagccacg tacgttgatg atcacctcgc aggtgttatt ccttgcggcg
     3661 gggatcaccc ttgccgtttc accttcccat gcggtttctc tgtttggtat cacgctgatt
     3721 tgcgccggtt tctcggtagg ttttggcgta gcgatgagtc aggcgttagg accattttca
     3781 ttacgcgcgg gcgtagccag ctcgacctta ggtattgcgc aggtttgcgg ttcgtcactg
     3841 tggatttggc tggcagcggt ggttggtatc ggcgcatgga atatgctgat cgggattctg
     3901 attgcctgta gcatagtgag cctgttgctg attatgttcg tcgcgcctgg acgccccgtt
     3961 gccgctcatg aagaaatcca tcaccacgct tgatctcaat ctgctgctct gtctgcaact
     4021 gctgatgcag gagcgcagcg tgaccaaagc ggcgaagcgg atgaacgtga ccccttcagc
     4081 ggtgagtaag tcgctggcaa agttaagagc gtggtttgac gacccgctct ttgtgaactc
     4141 accgctgggc ctgtcgccca caccgctaat ggtcagcatg gagcaaaatc tggcggaatg
     4201 gatgcaaatg agcaatctgc tgctggataa accgcaccac cagacaccgc gcggcctgaa
     4261 gtttgagttg gcggcggaat cgccgctgat gatgatcatg cttaatgcgc tgtcgaaacg
     4321 gatctaccaa cgttacccgc aggcgaccat caaactacgt aactgggatt acgattcctt
     4381 agatgccatt actcgtggtg aagtggatat tggtttttcc ggtcgcgaaa gccatcctcg
     4441 ctcgcgggag ctgttaagct cgctaccgtt agccattgat tatgaagtgc tgtttagtga
     4501 tgtaccctgc gtctggttac gcaaagatca tccggcactg catgaaacgt ggaatctgga
     4561 caccttttta cgttatccgc atatcagcat ttgctgggaa cagagcgaca cctgggcgct
     4621 ggacaatgtg ttacaggagc tggggcgcga acgcacgatt gctatgagtc tgccggaatt
     4681 cgagcagtca ctgtttatgg cagcgcaacc cgacaatctg ctactggcga ccgcgccgcg
     4741 ctactgtcag tactacaatc aactccatca actgccgttg gttgctcttc ctctcccgtt
     4801 tgacgaaagc cagcaaaaaa agctggaagt tccttttacc ctgctgtggc ataaacggaa
     4861 cagccataat ccgaagatcg tctggttacg ggaaaccatt aaaaaccttt acgcgtcgat
     4921 ggcataaccg aatcgtatga aacgcgaccc aatttcacaa taaaatgtaa aaaagttgta
     4981 acaagcttgt ctgaatcgaa cttttagccg ctttagtctg tccatcattc cagtaaatga
     5041 ttactcttgt attcataatg gaccattaag catggagcga aaaatggcga ctcactttgc
     5101 ccgagggatt ttaacggaag gacatctgat ttctgttcgt ctcccctccc agtgtcatca
     5161 agaagcccga aacattcccc ctcatcgtca aagccgtttt ctggcgtcca gaggtttact
     5221 cgcagaactg atgttcatgc tgtatggcat tggcgaattg ccggaaatcg tcaccctgcc
     5281 gaaaggtaaa ccggttttca gtgataaaaa tttgccttcg ttttctattt cctatgccgg
     5341 gaatatggtt ggcgtggcgt taacaaccga aggtgaatgt ggcctcgata tggaactaca
     5401 gcgtgcgacg cgcgggtttc atagcccaca cgcgcccgat aaccacacct tttccagcaa
     5461 tgaatcgcta tggatcagta aacaaaacga tcctaacgaa gcgcgggcgc agctcatcac
     5521 gctgcgccga agcgtgctaa aactaaccgg tgatgttttg aatgacgatc cgcgcgatct
     5581 gcagctgctg cccattgccg gacgcctgaa atgtgctcat gtaaatcatg tagaagcgtt
     5641 atgcgacgcg gaggacgtgc tggtgtggtc cgtggcggtc acgcccgcga ttgaaaagct
     5701 cagtgtctgg gagttagatg gcaaacacag ctggaaaagc ctgccggata ttcacagccg
     5761 cgccaacaat cctaccagcc ggatgatgcg ttttgcccag ctctctaccg tgaagtcttt
     5821 ttcgccaaat tgatagacaa ccacaggagt cattatgtct gaaaaattgc aggtggttac
     5881 gttactgggg agcctgcgca aaggctcatt taatggcatg gttgcacgta ccctgccgaa
     5941 aattgctccg gcgagcatgg aagtcaatgc gttaccttcc attgccgaca ttcccctgta
     6001 tgacgctgac gtacagcagg aagaaggttt tccagcaacg gttgaagctc tggcggaaca
     6061 gatccgtcag gctgacggtg tggtgatcgt cacgccggaa tataactact cggtaccggg
     6121 tgggctgaaa aatgccatcg actggctttc ccgcctgccg gatcaaccgc tggccggtaa
     6181 accggtgttg attcagacca gctcaatggg cgtgattggc ggcgcgcgct gtcagtatca
     6241 cctgcgccag attctggttt tcctcgatgc aatggtgatg aacaagccgg aatttatggg
     6301 cggcgtgatt cagaacaaag ttgatcggca aaccggagaa gtgattgatc agggtacgct
     6361 ggaccacctg accgggcaat tgaccgcatt tggtgagttt attcagcgag ttaagatcta
     6421 aataaaaaac ccgccagtaa tcatgcatgg cgggttttta atgcgctatc gattttaatg
     6481 agcgtcgata aatacaatct tcaggataaa cagcagcgca acgatgatta cgcacgggct
     6541 aaggtcacgc agacgcccgg taccaatctt catcacgcag taggagataa agcccagcgc
     6601 aataccttcg gtaatcgaaa agctgaacgg catcatcacg gcggtaataa acgccggaac
     6661 agattcagta agatcctgcc agttcacgcg agcaagactt gaggtcatca acacgccaac
     6721 gtagatcaac gcgccagctg cagcgtagcc tggcaccatt cccgccagcg gcgacagaaa
     6781 gataaccagc aggaacagca gaccaacaac cactgccgtc agaccggtac gaccgccaac
     6841 cgatacgccg gaagaggact caatataagc cgtaacggaa gaagtaccga taaacgaacc
     6901 ggtcacggaa gagatactgt cgacatacag cgcctgcttc atgcgcggga atttcccctt
     6961 ctcatccgcc agacctgctt tatcggtcac gccaatcagc gtaccggagg agtcaaacaa
     7021 gttgaccaac atgaaagaga aaatcacccc tgccagcccg aggttaaacg acccggctaa
     7081 atctacatga cccacaactg tcattacgct cggcggcgca gaaacgatgc cattgtagtg
     7141 cacatcaccc agcatccagc ccagcagcgt cgtcaccacg atagaaacca gcaccgctgc
     7201 gtgaatgttg cgcgaggcca gaatggcgat gatgaagaaa ccgaggatcc ccagcagcac
     7261 gctgtgagaa gtcagattac cgatgctcac cagcgtttcc gggttagcga caatcacacc
     7321 tgcgtttttc agccccatca tgccaatgaa cagaccgata ccgctggtaa tacccacacg
     7381 cagacttacc ggaatgttag caatcatcca gtaacgaacg cggaaaatgg tcaaaagaag
     7441 cagtccgaca gcgccccaga agattgcgcc cataccgatc tgccacggca gccccattgc
     7501 ctggacaacc acaaaagcga aaaaggcgtt aagccccatc gcaggagcca gcgccaccgg
     7561 aaggtttgca aacaatccca tcataatgct accgaaagca gcaatcagac aggttgttac
     7621 gaagacggca ctggtgtcca tgccagcaac gcccagaatt tgtgggttaa caaatacgat
     7681 gtagaccatc gtcaggaagg tggtgaaccc ggcaatcact tcagttcgtg ccgtagtacc
     7741 atgttcgcgc agtttaaaaa ctcgttccag catcccctga ccagaagtct gggtggtatg
     7801 ttgatgactc attatctatt tccgaacaag gagggaaaat ccgtcgctat cgtataccaa
     7861 aatgcgacaa taggcgcgtt tgtgagagac tttttttatt ggatttactt atacggcaac
     7921 gattgcgttg cgcaaatcgg ctctacgaaa acgttaaact gattaaaaag gaaaggcatg
     7981 tcccggatag aagcggtatt tttcgactgc gacggtacgc tggtcgacag tgaagtcatt
     8041 tgctcccgcg catatgtaac gatgtttcag gaatttggta ttacgctcga tcctgaagag
     8101 gtattcaaac gtttcaaagg tgtaaaactg tacgaaatta tcgatattgt ttcccttgaa
     8161 catggtgtta cgttagcgaa aacagaatct gaacacgttt accgtgcaga agtcgctcgg
     8221 ctgttcgatt cagaactgga agccatcgaa ggggctgaag cgctcctgtc agcgatcact
     8281 gcgccaatgt gtgtggtatc taacggccca aataacaaaa tgcagcattc tatgggcaag
     8341 ctgaatatgt tgcactactt cccggataaa ctgttcagcg gctacgatat tcagcgctgg
     8401 aagccagacc cggcgttaat gttccatgcg gcaaaagcga tgaatgtaaa tgtagaaaac
     8461 tgcattctgg ttgatgactc agttgccggt gcgcaatctg gtatcgacgc aggtatggaa
     8521 gtgttctact tctgcgccga cccgcacaat aagccgatcg ttcacccgaa agtcaccacc
     8581 tttacccatc tttcgcagtt accggaactg tggaaagcgc gtggttggga tattacggca
     8641 tagttcttca cactcccttc acttaccccg cttaaattgg cgctcaaagg taagtaaagg
     8701 gagtttgata tgtctgtttc acgtcgggta atacatcacg gactttattt tgcggtttta
     8761 ggaccgttaa ttggtgttct gtttcttgtc ctctacatat tcttcgcaaa agaaccgctg
     8821 gttcttttgg taataataca ggtacttcca ttgtttctct tattgtcgat tactacggga
     8881 gctattcctg cattgttaac cggcgtgatg gtcgcctgtc tgccggaaaa gatcgggtca
     8941 cagaaaaatt atcgttgtct ggctggtggc ataggtggcg tcgttatcac cgagatctat
     9001 tgtgcagtta ttgtacatat taagggcatg gcttcctcgg agttgtttga aaacattctt
     9061 tctggtgaca gtctcgttgt ccgcatcatt cctgcattgc tggcaggtgt ggtgatgagc
     9121 agaatcatta cccgtctgcc cggattggat atttcatgtc ctgaaacaga ctctttaagt
     9181 taagcgggat actttatctt tgggctactc aaaagcagac aggatgttac
//
LOCUS       DAKISA010000143         9083 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.144, whole genome shotgun sequence.
ACCESSION   DAKISA010000143 DAKISA010000000
VERSION     DAKISA010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9083
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.144"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..539
                     /locus_tag="OVW45_003835"
     CDS             <1..539
                     /locus_tag="OVW45_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCV5528173.1"
                     /translation="NLKITSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVED
                     YTVHEITLGPGCNVPGYAGTTIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVTGVAN
                     SSGFALAALLNANIELGNDPIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPS
                     SLPELFELKNGEWNYIGK"
     gene            complement(680..1453)
                     /gene="uxuR"
                     /locus_tag="OVW45_003836"
     CDS             complement(680..1453)
                     /gene="uxuR"
                     /locus_tag="OVW45_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCV5528174.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWRHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1668..3128)
                     /locus_tag="OVW45_003837"
     CDS             complement(1668..3128)
                     /locus_tag="OVW45_003837"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCV5528175.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3209..4393)
                     /gene="uxuA"
                     /locus_tag="OVW45_003838"
     CDS             complement(3209..4393)
                     /gene="uxuA"
                     /locus_tag="OVW45_003838"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCV5528176.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            4733..6076
                     /gene="gntP"
                     /locus_tag="OVW45_003839"
     CDS             4733..6076
                     /gene="gntP"
                     /locus_tag="OVW45_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCV5528177.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDTATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGQLELVNSVVGLIIVLIISMVA"
     gene            complement(6240..7142)
                     /gene="fimH"
                     /locus_tag="OVW45_003840"
     CDS             complement(6240..7142)
                     /gene="fimH"
                     /locus_tag="OVW45_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCV5528178.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSVGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7162..7665)
                     /gene="fimG"
                     /locus_tag="OVW45_003841"
     CDS             complement(7162..7665)
                     /gene="fimG"
                     /locus_tag="OVW45_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCV5528179.1"
                     /translation="MKWRKRGYLLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNSGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(7678..8208)
                     /gene="fimF"
                     /locus_tag="OVW45_003842"
     CDS             complement(7678..8208)
                     /gene="fimF"
                     /locus_tag="OVW45_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HCV5528180.1"
                     /translation="MRNKPFYLLCAFLWLAVSRALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSALSWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8218..>9083)
                     /gene="fimD"
                     /locus_tag="OVW45_003843"
     CDS             complement(8218..>9083)
                     /gene="fimD"
                     /locus_tag="OVW45_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HCV5528181.1"
                     /translation="KSYCLNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLA
                     GVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQL
                     YYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYAT
                     EYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPL
                     PFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQ
                     QLLTQLSAECR"
BASE COUNT         2117 a         2405 c         2310 g         2251 t
ORIGIN      
        1 ggaacttgaa aatcacctca gctttagacg aaatatatgg tggcatatgt aaaaaggcgc
       61 atgctgccac agagttagca gaaggtttac ggctaaattt atttatgaag agccctttcg
      121 atcctgttga ggactataca gttcatgaaa taacattagg ccctggttgc aatgttcctg
      181 gttatgccgg aaccacgata ggatatattt caacccttcc agcttctcag gcaaaaagat
      241 ggaccaatga gcagccaagg attgatatct atattgatca gataatgacg gtaacagggg
      301 tagcaaattc ttccggattc gcccttgccg cattacttaa tgctaatatt gagttgggta
      361 atgacccaat aattggtata gaagcctatc ccggtaccgc ggagatccat gcaaaaatgg
      421 gatataaagt tatcccgggt gatgaaaatg caccactcaa acgtatgact ctgcaaccgt
      481 catctcttcc agaactgttt gaattaaaaa atggtgagtg gaattatatt ggtaaataac
      541 taccaggaag aatgagtact aacattcagg ttatacagat agctaaacaa taccctgcaa
      601 tttacaaagt aatactcatt gcgacttctt aatttaagaa agcgcaagga acatttaccc
      661 ttgcgcttat tataataagt caggcgtcaa ctttatccag cggccatgaa tcaaacagat
      721 agccatcaaa ataaatatcg tcaacgttcg agaattccag cagacgttgc ttaacgtttt
      781 ccagatgccg ccacattgcc agcttcgccg ctcgggcatc ttttttgatt aacgcggcga
      841 ggatctgttt gtgatcgccc aaccactctt tgcgatacag gctgtcatcc agatggctgt
      901 gcaactggat ccacattgga ttgttttccc gccactgcca ggactgacgg aacagctcca
      961 ccagcatgct gttatgcgtt gcttcggcaa tggcgagatg gaactgcata tcaccacttt
     1021 cgctgctgcc cggcgcgctg gaagccagct cacgctcttc cagttgcaat gcctgacgca
     1081 ttttgacgat atcttcgcgg gtagcctgca aagcggcaaa ttccgcgatg ttactttcca
     1141 gtaactgacg cgcctgtaac agctcaaaag gaccggcatc attgcagacg ttagcatccg
     1201 gactgtctgt gttgtggctg cctgagctat caagaacata gataccggca ccccggcgca
     1261 cttccaccag ccctttgatc tccagcatga tcagcgcttc acgtaccacc gtcctcgtga
     1321 catcaagcat ttctgcaatt tcacgctccg gcggcagccg ttcgccagga ttgtatggcg
     1381 tctttatgat cagatcgcgg atcatcgccc cgacttcctg gtaaggtctt tgcgcagagg
     1441 tggcagattt catggtgttg tgtccgttag gtgcggttaa tcaaattttt gccggatgcg
     1501 gcgtgaacgc cttatccggc ctacaacggg caattttacg gcacccgtta atctcaaact
     1561 ataccattag cactggaagt tggcacgtac aggtgggcaa aaaaacgaaa actggtcaac
     1621 caaatatcag tttgattgac cagtaagtct gtagggtaat cagttagtta cagcgcagcc
     1681 acacactcgc gcgcaccgcg ttcgcacagc tgctgatatg ccgccgtcac cgcgccaaca
     1741 aagtcggcat tctgcggcag atcatcggca aaaatgccgc tcaggcccag cagcgctttc
     1801 acgcggtctg cgccctgata ctgcgcgtta atcttctgga actcggccag catcgggtca
     1861 accacgtcaa tggcattacc ctgttcatcc acgccctggg tgtagcgcat ccagccagcc
     1921 acgcccagtg ccaggtgacg ccagctgccg ccgttttgca ggtgcagacg caccggatcc
     1981 agaagacgct gcggtaactt ctggctgccg tccattgcaa tctgccaggt acggtgacgc
     2041 agagacgggt tgctgaaacg ctcgatcagc agcgtcgcat aggcgttcag gtctgtacct
     2101 tccggcatcg acagcgttgg cgcttgttcc tgcatcatca gggcaaaggc cgctttgcga
     2161 taatccggat tagtcatggt gtcggcaatg gtttcatagc cgccgaggta accgaggtac
     2221 gccaggaaag agtggctgcc gttcagcata cgcagtttca tcatttcgaa cggcacaacg
     2281 tctgcaacga actgtgcgcc cactttatcc cagtccggac gaccattaac gaagttatct
     2341 tcaatcaccc actgacggaa cggttcgcag gcaatggcgc acgggtcgta aacacccagc
     2401 tggtcagcaa tttcctgtaa ggtttctggc gtcgccgccg gaacgatgcg gtcaaccatg
     2461 gtgcacggga aggtgacgtt ctcttcaatc catgccgcca gctgcggatc acgtgcctga
     2521 gccagcccca gaaccgcgac cttcgccaca tgaccgtttt cacgcacgtt gtcgcaggac
     2581 atcaccgtaa acgctttcag ccctttttca cgacgcagac gcaaggcttc gacgatgtaa
     2641 ccaatcgcgg acttcggcgc agtcgggttt tccagatcgt gcttgatcag cgggttattg
     2701 agatccagct gaccgcttgc cgcatcggcg caataacctt tctctgtgac cgtcagggag
     2761 acaatcgccg tctgcggacg cgccatcgcg ttgagaatac cttcacaacc gtcgatttcc
     2821 ggatgcaggg cttctttcat cgaaccgata attttcagct cggtgctttc tgcgcctttt
     2881 tccgccacgg tgtacagcag ttgctgtttt ttcaggtttt caatcagcac acggtcgttg
     2941 cctggcatca ggttaacttc gcagataccc cagtcgctgt cggtactttc cagcagatga
     3001 tgggtgtaca gcgcctggtg cgcgcggtga aacgccccgc aaccgagatg cacaatgcgt
     3061 gattccagac gagaatgatc ccatgacggg cgggcaaccg gcagattgct gtcaacaata
     3121 gtagtcatcg taaactccag aatccgtctt cagagagtga cggcaaggaa gaaggaaccg
     3181 gcacggcgcg cagccatgcc ggtgggtatc aacggctaaa gaaagcgcgc tggatcgcca
     3241 gttcgacacc gcgaacttca gccaggcctt tcagacgacc aattgcggag taacctgggt
     3301 tggttttctt cttcaggtcg tccagcatct gatgaccgtg gtccggacgc atcgggatca
     3361 ggtcttcttt gccttccgct ttacgacggt gttcttcttc aacaatcgct ttcaccactt
     3421 cgtacatatc aacgtcaccg ttcaggtgcg ccgcttcgtg gaaggttttc gggttatctt
     3481 cacgcatggt ggagcgcaga tgggtgaagt aaatacgcgg accgaactgc ttgatcatat
     3541 caaccagatc gttgtcagca cgcacgccgt aggaacctgt gcacatggtg aaaccgttcg
     3601 ccatgctgtt tacggtatca accatccact gcatatcttc aatggtggaa acaatgcgcg
     3661 gcaggccgag gatcgggcgc ggcggatcgt ccgggtgaac agccatacgg acgccaactt
     3721 cttcggcaac cggaataatc gctttcagga agacggcaaa gttttcgcgc agtttggctt
     3781 tgtcgatatc tttatacagc tccaggtgct tacggaactg gtcgagggta tacccttctt
     3841 ccgcgcccgg cagaccggca atgatgttac gggtcagacg cgctttgtct tcgtcgctca
     3901 tagtggcgaa gcgttcagcg gcctgagcaa tttcttcttc ggtgtaatcc gcttccgcgc
     3961 ctggacgctt caggatatgc atttcgaatg cagcgaattc gatctggtcg aagcgcagag
     4021 ctttggagcc gtctggcagc acgtattcga ggtcagtacg ggtccagtcg agcaccggca
     4081 tgaagttgta gcacacggtg cgaatgccgc actgcgccag gttgcgcaga gtctgctgat
     4141 agttagcgat ccactgttca tagttgccag tgtgggtttt gatatcttcg tgaattggca
     4201 cgctttctac gacagaccat accaggcctg cgtcttcaac gatcgccttg cgtttgagga
     4261 tctcttctac ggaccatact tcgccgttcg ggatatggtg cagcgcggta accacgccag
     4321 ttgcgcccgc ctgacggaca tcagctagag aaaccggatc gtttgggccg taccagcgcc
     4381 aggtctgttc catgattttt cctctttcac atagtgggtt ccacagcaaa gagacgtgga
     4441 gccccggagt tgtttaggta tccggatgaa caaccagacc gcagccgtgg cgttgcatcg
     4501 gatgagatgg cgtataagtt ctaccttttg gtaagacatg tcaacccgaa atctgaaatt
     4561 ggttaaccac atcgcaagaa tttcactctc acggttgttg ctgcaaattg cagcaaaggg
     4621 taatcgatgg ttattgatca gaggatgtga cattcatcgc aacaatggtt gaccaattta
     4681 cataacatgt cgcccaaaat aacaacggtt caaccaggta aggtaaaacg ttatgcatgt
     4741 gcttaacatt ctctgggtgg tattcggcat tggcctgatg ctggtactga atttgaagtt
     4801 caaaatcaat tcaatggtgg ctttgctagt ggcggcgctg tccgtcggga tgctggcggg
     4861 catggatttg atgtcgctgc tgcacacaat gaaagcgggc ttcggcaaca cgctggggga
     4921 actggctatc atcgtggtgt tcggtgcggt catcggtaaa ttgatggtcg actccggcgc
     4981 ggcgcaccag atagcgcata cgctgctggc gcgtctcggt ctgcgctatg tacagctgtc
     5041 ggtaattatc atcggcctga ttttcggtct ggcgatgttc tatgaagtgg cctttatcat
     5101 gttagcgccg ctggttattg tcattgccgc cgaagctaaa atcccgtttc tgaaactggc
     5161 gatcccggca gtagcagctg ccactaccgc acattcactg ttcccaccgc agccgggtcc
     5221 ggtggcgctg gtgaatgctt atggcgcgga tatggggatg gtttatatct atggcgtact
     5281 ggtgacgatc ccaagtgtca tctgcgcagg tctgatcctg ccgaagttcc tcggcaatct
     5341 tgagcgccca acgccatcat tcctgaaagc agatcaaccg gtagatatga ataatctgcc
     5401 ctctttcggc gtttcgattc tggtgccgct gatcccggcg atcattatga tctctaccac
     5461 catcgccaat atctggctgg taaaagatac ccctgcctgg gaagtggtta actttatcgg
     5521 ttcctcgccg attgcaatgt ttattgcgat ggtggttgca ttcgtactct tcggcaccgc
     5581 gcgcgggcac gatatgcagt gggtgatgaa cgcttttgaa agcgcggtga agagcattgc
     5641 aatggtgatt ctgatcatcg gtgcgggtgg cgtgctgaag cagaccatca tcgacaccgg
     5701 cattggtgac accatcggca tgttgatgtc ccacggcaat atctcgccct acatcatggc
     5761 atggctgatc accgtactga ttcgtctggc gacgggtcag ggtgtggttt cagcgatgac
     5821 cgccgccggg attatcagtg ctgcaatcct tgatacagca actggtcagc tggttggcgt
     5881 gaatccggcg ctgctggtgc tagcgacggc tgcgggttcc aacaccctca cccacattaa
     5941 tgatgcctca ttctggctgt tcaaaggtta ctttgacctg tcagtaaaag acacgttgaa
     6001 aacctggggt cagctggagc tggtcaactc cgtggttggg ctgattattg tgttgattat
     6061 cagcatggta gcgtaaaaga gcaccagagc ctgccattgg caggctctgg tcggattgtc
     6121 ggcaaagtgc gctttgttta ttcttatctg gcctacaaag ggctaacgtg cgggtttgta
     6181 gatagcgtag cgcatcaggc aatattgcgt acctgcatta gcaatgtcct gtgatttctt
     6241 tattgataaa caaaagtcac gccaataatc gattgcacat tccctgcagt cacctgccct
     6301 ccagtacgtg cgtaatttgc cgttaatccc agacttaccg ccgaagtccc tactgctcct
     6361 aacgataccg tgttattcgc tggaataatc gtaccgttgc gcgtcaactg tacgccgacg
     6421 ccctgcgctg gtgaaaacga ggcggtattg gtgaaaatcg aattgcccgc atctgcggtt
     6481 gtgccggaga ggtaatatcc caggttttgg cttttcgcac aataaacggt aagaggaatt
     6541 ggcactgaac cagggtagtc cggcagagta acggtgacat cacgagcaga aacatcgcag
     6601 ccgccagtgg gcaccaccac atcattattg gcgtaaatat tccacacaaa ctgaaaatca
     6661 tcgctgttat agttgttggt ctgtcgcaaa ataagcacgg caattaatga accagcttta
     6721 atcgccaccc cgccaacact gctcacaggc gtcaaataaa gcgccaccgg ccacggctta
     6781 tccgttctcg aattataaac aacccgcggc gtttcgctgg tggtaggaaa tggatagcta
     6841 ctgccactat attttacggt cccggaaaaa ttagataaca cgccgccata agccgagcct
     6901 cgttgcagtg tgacatagtc tgtaatggtt tccggataat cgttatggca aaagatttgc
     6961 gtcgaaagat ccacgaccag gttttgcccc acattcacgg caggcgcaag gtttacataa
     7021 acattagcgc tgccaccgcc aatagggata gcggtgccat tggcggtttt acaggcgaat
     7081 gaccaggcat ttaccgacca gcccatcagc agtacagcaa acagggtaat aactcgtttc
     7141 attacaatca tctcttcggg ttcagctgta ggtataggtg atgctaatca ctgcctgaat
     7201 ggttccctga gtggctccgc catttaccgt caatgctctg acctgtaacg ggaagtgcgc
     7261 tgattgtgag gaatcatcca cctgaactgt tttggttgcg ccactattca atgtgttgcc
     7321 actgtcatcc tgtagctcta actggatgtt ttgcgcggtc ccctggtttt tataatatcc
     7381 ggtactgtcg gctgccccgc tgaagctggc agtgaccctc gacgttccca ccggacaatt
     7441 agtcaactca agcgcaacat catgccaggc cgatgccgcc ccggcagaca taagactgaa
     7501 agaataaaga tcgccgagat ctaccgtggc attggtggtg gaaaccgtgc acggtttggc
     7561 gacgacctta ccgttcaccg tgatggtgac atcggctgcc tgtatcgtcg cacttgcgag
     7621 cgccaacatt gccgccaata aatacccacg tttgcgccat ttcatgagca tctccagtta
     7681 ctgatattca agagtgaagg tagccgtggc attgatatgc cccgcagtga caggcacctg
     7741 tgtcgccatt agccgggcgt aaaaattcag cgtatttggt ttacccggcg tcagggtcgt
     7801 ccacgaaagc gcggacgatg gggcattaag gggtatctga ttttgttgct cattcagaag
     7861 ctgtattccc agtcccgcag ccgctgacac cgtattttca agtgcaagca ggttggcatt
     7921 gtggctatct gcaacaccgg taaacccaac ttttacggca gaaacggcat taccacaggg
     7981 tgacagcaaa atacgaaatg gaacgacagg agtcgtcgcg ccaatgttgt taaattgctt
     8041 cgccgcgttt tccatcagat caacagtaaa attggttgat tcagcggcca cactacagcc
     8101 gttatctctg acatagccgc ggatagtaat cgtgctatcc gcagccaaag cgcgactcac
     8161 cgccaaccac aaaaaagcgc acagaagata aaaaggtttg tttctcatca cgccccctta
     8221 acgacattca gctgatagct gggttaataa ctgctgctga ctctctggtg gcagttgata
     8281 attggcgaca cagtgagcat tttcctcttc tccccatttc acctgaactt ttcccgctaa
     8341 aggcattccg ctgaggtaaa cctgaccatt atccgcaaca atgccgctac tctggctact
     8401 ctctgatgtc accatcgccc caaacggcag cggcttatta ttgtgggtta gcgtcatgag
     8461 cagttttatc ccaacgcgcg ctttaaactc tgctcgcacg atcgccccac gagtgggaac
     8521 aacgttagcg accgcgttat ctaaatcgac gttatcagcc agggtattgg tatccagcgc
     8581 tactctgttt tcccgatatt cagtggcata aggcagcacg gcataaccgc gccagtcggt
     8641 acgcaccccc gtctggtttt cgacttttgc atcttttgcg ccaggcgctt taacaagcac
     8701 caccgtatcg tttaacggct gccccagcgt tacgccattg gcatgagcca gtaccccacc
     8761 gctgactccg taatagagct gcttaatatc atcgctatgg ctgtaaccga tgttggcatt
     8821 gccgtaacca ccgcgataat tcagcgtggc gtagcctgta cttccgctat taccatcacc
     8881 tcccccggcg taaccggttt gcacgctata gctgaggttg ttgtcttcca gcaacgtacc
     8941 gtatacaccg gccagattgg tcatccgacc gttgagatcg tgtgacatgc tgtaactggc
     9001 actggcatgt cgccactgag atttactgtc agaacgcagc cagtggctga aaggaatatt
     9061 gacgttaagg cagtatgact ttc
//
LOCUS       DAKISA010000144         9016 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.145, whole genome shotgun sequence.
ACCESSION   DAKISA010000144 DAKISA010000000
VERSION     DAKISA010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9016
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.145"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..270)
                     /locus_tag="OVW45_003844"
     CDS             complement(<1..270)
                     /locus_tag="OVW45_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HCV5528182.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQD"
     gene            749..2527
                     /locus_tag="OVW45_003845"
     CDS             749..2527
                     /locus_tag="OVW45_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HCV5528183.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQFEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTCRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(2567..3046)
                     /gene="xdhC"
                     /locus_tag="OVW45_003846"
     CDS             complement(2567..3046)
                     /gene="xdhC"
                     /locus_tag="OVW45_003846"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HCV5528184.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(3043..3921)
                     /gene="xdhB"
                     /locus_tag="OVW45_003847"
     CDS             complement(3043..3921)
                     /gene="xdhB"
                     /locus_tag="OVW45_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCV5528185.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLADGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEAPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(3932..6229)
                     /gene="xdhA"
                     /locus_tag="OVW45_003848"
     CDS             complement(3932..6229)
                     /gene="xdhA"
                     /locus_tag="OVW45_003848"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCV5528186.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFAPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPSAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            6644..7399
                     /locus_tag="OVW45_003849"
     CDS             6644..7399
                     /locus_tag="OVW45_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCV5528187.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            7478..7547
                     /locus_tag="OVW45_003850"
     tRNA            7478..7547
                     /locus_tag="OVW45_003850"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7510..7512,aa:Gly,seq:ccc)
     gene            7740..>9016
                     /locus_tag="OVW45_003851"
     CDS             7740..>9016
                     /locus_tag="OVW45_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528188.1"
                     /translation="MEYFMKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYM
                     STLDQIKRMQDVISNPKRLDGVPGHMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQND
                     ALGLYLDLLIQAIDTGTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDAR
                     LNTSSVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYE
                     RITLQLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAG
                     PYGIKRYEKDNYQSANFWFNDIKTDTDQNSHAK"
BASE COUNT         2382 a         2052 c         2240 g         2342 t
ORIGIN      
        1 atcttgttgt gcaaggccga gcaggttaag cgcggaagca taagagaagc gggtacgggt
       61 ggagttgtcg cggaatacgg aaatacctaa tccactatta aagactttgg ttgagatgtt
      121 ttcagcacgc agtgctttta atgctgcggc aacgtccagt acttgtttca gttcatctgg
      181 cgtctgttcc cacgttaaca aaaaatcttt ctcgtgaagg tgagaggtca gcgaattgat
      241 atccttaatc agctcattaa cagttttcat catacagtcc cggtagtgga atgacaggca
      301 aagtggttta tttcactgtg acttgccaaa gtgattggtg ttttcatagc tatacctggg
      361 actaacaata agtgtgccag aattatccgc gcggctgcaa aagactaatt tgacggataa
      421 tgtgtggggt tgttcacaaa ttgatgccag cttctggttg tttgtaaaat acgaaaacgt
      481 ttggcttttt ttatcgcgtt atttatttgc tgctaagtat cagaaataag ataaaggggc
      541 aggctatctc tcttcagttt gataaactca ccgccgtaat tctcatattg atatagaaat
      601 gtgatatctc ttccataatt tcattagaaa atgataaaaa tcttatgttt ttatcaaagc
      661 caatttcgcc tgagttaata tcatcatgat ctgtgaacca tcaacgtctt caatgatcga
      721 cttttaagta agtctggaag gtaaagctat ggagcttgct actacgcagt cagtattgat
      781 gcaaattcaa ccgacaattc agcgttttgc cagaatgctt gccagcgttt tgcagtttga
      841 ggttgagatc gttgatgaaa acttgtgtcg cgtcgccgga acgggcgcgt atgggaagtt
      901 tcttggtcgc cagttgagcg gcaactcacg cctgctccgc cacgtcctgg aaacgaaaac
      961 tgaaaaagtt gtgacacagt ctcgcttcga tcccctttgc gaaggttgcg atagtaaaga
     1021 aaattgccgc gaaaaagcat ttctgggtac gcctgtcatt ttacaggatc gttgtgttgg
     1081 ggtgataagt ttgattgccg ttacccacga gcaacaagag catatcagtg ataatttacg
     1141 cgaattttct gattatgttc gccatatatc caccattttt gtttcgaaac ttctggagga
     1201 tcaggggcca ggagataaca tcagtaaaat attcgcgacc atgatcgata atatggatca
     1261 gggcgtatta gttgttgatg atgaaagtcg ggttcagttt gttaatcaga ctgccttaaa
     1321 aacacttggt gttgtacaaa ataatattat tgggaaacct atccgtttca gaccattaac
     1381 atttgagagt aattttactc atggacatat gcagcatatt gtttcgtggg acgataaaag
     1441 tgaattaatc attggtcaat tgcataacat tcagggccga caattatttt taatggcatt
     1501 tcaccaatcg cataccagtt tttctgtagc aaatgcacct gatgaaccac atattgaaca
     1561 attggttggc gagtgccgtg ttatgcggca attaaaacga ctcattagcc gtattgcacc
     1621 cagcccatcc agcgttatgg tggttggtga aagcggcacg ggtaaagaag tcgtcgcccg
     1681 agcaatccat aagttgagcg gaagacggaa taaacccttt attgctatca actgtgccgc
     1741 gattccggag cagcttctgg aaagcgaact gttcggttat gttaaaggcg catttactgg
     1801 tgcttctgcc aacggtaaaa cagggttgat tcaggcggcg aatacgggca cgctgtttct
     1861 cgatgaaata ggtgatatgc cattaatgtt gcaggctaaa ttactgcgcg ctattgaggc
     1921 gcgtgaaatt ctgccgattg gtgccagtag cccaatacaa gtcgacattc gcatcatttc
     1981 tgcaactaat cagaatttgg cccagttcat tgccgaaggt aaattccgcg aagatctctt
     2041 ctaccgactt aatgttatcc cgataactct gccaccgctg cgtgaacgtc aggaagatat
     2101 tgaactattg gtgcattact ttttacatct gcatacctgt cgtctgggat cggtttatcc
     2161 tggcattgct cccgatgtcg tcgaaatatt gcgtaagcat cgttggcccg gaaacctgcg
     2221 cgagttaagc aatttgatgg aatatctggt taacgtggtt ccttcaggtg aagttatcga
     2281 cagcacgcta ttgccgccaa atctgctgaa taatggcaca acggagcaaa gtgatgtaac
     2341 agaggtcagt gaggcgcacc tgtcactcga tgatgcgggc ggcacggcgc tggaggagat
     2401 ggaaaagcaa atgatccgcg aggcgctttc acgtcataac agcaagaagc aagttgctga
     2461 tgaactgggc atcggcattg ctacgctcta tcgcaagatt aagaaatatg agttgttaaa
     2521 cacataacct cagtgaatcc ggcctgaatt caggccggat atccttttac ttcgttttct
     2581 cgcaatccag aactgtattt acaatcatct gataccccgt gcagcgacaa agatttcccg
     2641 ccagtccgcg acgaatttcc gtaatggtta atggcttctc gcgtggtttc gccagcattg
     2701 ccgtggtagc cataatcagg ccaggcgtac aaaacccgca ctgcactgcg ccggatttcg
     2761 cataagcctg ctgaacatga gaaagttttc cgcctttcgc ttcaccttcc agcgtgcgga
     2821 tctcctttcc ttcagcccag gcggcaaggt ataagcaact gtctattgct gtgccgtcga
     2881 ccaacaccgt acaggcacca cattcaccca cgcagcaccc ttgtttgaca cttagcagtc
     2941 cttgttcgcg gagtaattcc gagagcggcg tgcctggtgc ggcgtgaagc tgaaaaggca
     3001 tcccgttaat ggtgcattcg atggtaattg tttcgctgtg attcattgca attttccccc
     3061 cgccgcggcg acggcttcgc taatcacttt tttggtcatc gtctggatga gatgcagacg
     3121 aaactcttta ctggcccgcc atgaagaacg cggggcgaca tcttgcagga cagattcgct
     3181 gatagcttcc agcgtttgca ggtttaatgg cgcattttgt gcagtctgtt cggcatgttg
     3241 gcagcgaatc ggcgttggcg cggcaacacc aaatgccagg cgtaattcgc tgaaattgcc
     3301 gttatccagt cggcaatgtg cggcgcagcc aatcgttgaa atatccattg cgtcgcgcat
     3361 ggcatattta aaatgcgcgc tgcccgcgtg ttctttcggc tgtggcggaa aatgaaaggc
     3421 gacgaggatt tcgtcatgct caagagacac tttgcccggc ccggtgtgaa agccattaat
     3481 cgggacgaaa cgaacaccgc gcggggagtg gatctccagt ttcgcgtcat aaattagcgt
     3541 tggcgtggca gaatctgcgc tggtggcacc gttgcaaata tttccaccgt aggtagcgac
     3601 gttacggatc tgcggtccag caatggacgt ggccgcagca cataacgccg ggagatgacg
     3661 ttgagttata ggagcttcta ttagctgggt aaatgtcgtt gcagagccga tacgtagcga
     3721 gccatcttcc gccagcgtaa ttccccgcag ctccgccaga ttatgaatat caacaatatg
     3781 gcgataacgg tcattgtgat ggtggagctg aatcagtacg tcagtgccat cggcgagcag
     3841 tttggcctgc gggttgtcag ccagcaggtt gatggcatcg gcaagggttg ctgcgcgatg
     3901 gtaagaagca aaatcaaaca tgatgttatc ctcaaatcaa tcctgccaga tggaactctt
     3961 catataaccg ttttggcgtc agcggcagtg tattgattgc aacaccggta gccatcttca
     4021 ccgcgttacg aatagcagcg gcaacaggaa ttattggtgg ctcacccagt gacttatgtc
     4081 cgtatgcgga ttgcggctca ttgatttcga cgaacgcgct ttccagttgt ggcagatccg
     4141 gcatggtcgg cattttgtaa tccagcagat tggggttacg gaccacgccg cttttagcat
     4201 cgatgatcat ctcttcaaat agcgcccagc caatgcccat tcccattccg ccgtgtacct
     4261 gaccttctgc cagcagtgga ttaagaatat gccctgaatc atgaacgttg aggatgcggt
     4321 tgatggtgac tttgcacagc gcaatatcga ccgtcagatc aacaaaggta cagccaaacg
     4381 ccgatgggtt agtggtggtt ttgatggagc tttcagcaga gagctgcccg ccgcgttcag
     4441 ggtggtagaa agcgtccatc gccaaatctt ttaacgacat taacggctct tccggtcgtt
     4501 caaccagcac gatatggcct tttatcaggg tcagattcat cgctgactga tgtagcatga
     4561 ctgcggcgtg agcgatgatt ttctctttta ataatagtgc cgcactgcgc agcgcaggcg
     4621 cggcaacata gctctggcgt gaggcaaatg cgccgggagc gaacggcgta acgtcggtat
     4681 cttgagttga aataacgcga acgtcgctga ccggaacccc cacggtttct gccaccattt
     4741 gcgagaagac ggtgtcggca ccctgaccga tttccgtcgc gccgctttgc acgttgatgg
     4801 ttccatcctg attcatcaga aggcgcgcgc ctgctatttc tacgccgaca ggccaggtgt
     4861 tagaggtata gctaaaacag gcgacgccaa cgccgcggcg caaattgcct tgctggttct
     4921 ggcattctgc acggcgtttt tcccattcaa agattttccg gcctttttca agacactccg
     4981 gcaaccctgc gctgtaaata cgtttgcccg tgagcggatt agcatctcct tcgcgggcgg
     5041 cgttgcgtaa acgaatttca acaggatcaa tacctaacgc tgtcgcggcg tcatcaagca
     5101 tagactcaac ggcaaatacg acttgtggcg cgccataacc acgcatcgca ccagccgagg
     5161 ggaggttggt atagcaggtc tttgaactgt aagcgtaggc acaacgagga taaaggtaag
     5221 cgactttatt cccccccgca gaagcgatgg agtgcccgtg agatgcataa gcgccggtgt
     5281 tagacagaac atccagacta taacctttca atgttccgtc gcggttcacg cccatttgcc
     5341 cgtcaatggt aaaagcgtgg cgggtacggg ttgcgaggaa acactcttca cggctaaggg
     5401 aaactttcac cggaatgccg ccaagcttgc tggtcaggaa tgctgccatt ggctcttcca
     5461 gtacatcctg tttattacca aaaccgccac cgacaaacgg tttgatgact cgtacgcatg
     5521 accagggaat atccagcgcc tgaccaacca cgcggcgaac aatgtgcggg atctgggtgc
     5581 tggaaacgat ggtaattcgc gagtcatcct ccatccacgc cagcgatgtt acgctttcca
     5641 tgtgacaatg ttgaataacg ggggtctgat agtgcccctg tacctggtag tcggcggcat
     5701 cgattgtttg ttggacatta cccgtcgaca tcgtgctttg tttcagtaaa ttgccaccgt
     5761 tatggattgg tgcagcgtct tctgccagcg ccgcttctgg cgtggtgata acgggtaatt
     5821 cttgccactc aatgctgacc aattgcgccg ctttttctgc cgtgagttca tcgcgggcca
     5881 cgacgatggc aacggcgtca ccatgatgac gaacatggcg agttagcagt gcgcgatcgg
     5941 cggtatcgcg cttgttttcg tcaagtgtcc aggcatgccc agctgtagcg aatggaatat
     6001 caggcacatc ttcccaggta aaaatcgcca gtacgcccgg caaacttctg gcttgttcat
     6061 cattaatact tacggcataa ccatgtgcga tagggctacg tacatatttc gcataacaca
     6121 tgcccgccat aacataatcg tcagtatatc gtgcccgccc ggtgacctta gcaatggcat
     6181 cgacgcgcat gcatgattca cccgtagcgg ttgcttcccg cgcttccata gaaatcccct
     6241 cgatttgaac atttggttct tatggcaaag atttagtcta agcaggcaag atttacgcca
     6301 gaaacgacga taagcgttag gggaggtagg aagtgtgatg acgttaaaaa aacacagata
     6361 tgatacgaaa caggactctg aataggggca gtgatacgta tcaatatgag tggcgataaa
     6421 atcaatgtga taaattggga ttattacgca tcaggatggg aaatagggta aaaagtcagg
     6481 aagttcgata cctctacagc gaatattttg ctgatgatac ttccctttgc caaatgaaat
     6541 taacctcttt ctctattacc acgtttttcc agaagcaaga gattgggtat caagaggctg
     6601 gctgctatga taaggcgtct tgttttttaa gcgaggaaag attttgagtg cgggacgcct
     6661 gaataaaaaa tctctgggta tcgtgatgtt gttatcggtt ggactgcttt tggcgggctg
     6721 ttcgggtagc aaatcatccg atacaggaac gtattccggc tccgtttaca ccgtgaaacg
     6781 gggggatacg ctatatcgta tttcgcgcac cacgggaacc agcgtaaaag aactggcgcg
     6841 actgaacggc atttcccccc cttacaccat tgaagttggt cagaaactaa aactgggtgg
     6901 ggcgaaaagt agcagtagta cacgtaaatc aaccgccaaa tcaacgacca aaaccgcatc
     6961 ggttacaccg tcatcagcgg taccgaaatc atcctggccg ccagtagggc aacgttgttg
     7021 gttatggcca acgacaggga aagttatcat gccgtattcg acagcagatg gcggcaataa
     7081 agggattgat atctcagctc cacggggtac acctatttac gccgcgggtg caggaaaggt
     7141 ggtgtatgtg ggcaaccagc tgcgtggcta cggtaatctc atcatgatta aacacagtga
     7201 agattacatt acggcttacg cccataatga cacgatgctg gtaaataatg ggcaaagcgt
     7261 gaaggctggg caaaaaatcg ccactatggg gagcacggat gcggcatctg ttcgcctgca
     7321 tttccagatt cgttaccgtg caacggcaat tgatccgcta cgttacttgc cgccgcaggg
     7381 cagcaagcca aaatgctgat ggcgaattaa tcagcagtca gcagcgcggc acttgctaag
     7441 gagagcgtaa ggtttataat gccttacgca tctcgaagcg ggcgtagttc aatggtagaa
     7501 cgagagcttc ccaagctcta tacgagggtt cgattccctt cgccgctttt gctgtatctg
     7561 attttctata ttatcttaat tcaatcgctt cagacttttc gcaatcgccg taatatttcc
     7621 ccattttgtt gtaaataaaa agcgtttacg caatgcaatc cttttgtaac aacccaggta
     7681 aaacacctta ttttcaagtt gttaaataag tggttattta cgcttttaac aaacgattga
     7741 tggaatattt tatgaaaggg aaatctgcat taactcttct gctcgctggt atattttcgt
     7801 gcggcacatg ccaggcgaca ggggcagaag tgacgagtga atctgtattt aacattctta
     7861 attccacggg ggcagcaaca gataaaagtt atttatcgtt aaatccagat aagtacccta
     7921 attatcgtct tctgattcac tcagccaaat taaaaaatga aataaagagc cactatacca
     7981 aagacgaaat tcaagggttg ttaacattaa cagaaaacac acgaaagtta acgttgacgg
     8041 agaaaccctg gggaacattt attcttgctt ctactttcga agatgacaaa acagcggcag
     8101 aaacccacta tgatgctgtc tggctgaggg atagtttatg gggctatatg gcattagtat
     8161 cagatcaggg aaatagtgta gccgcaaaaa aggttctgct caccctgtgg gattatatgt
     8221 ctacgctcga ccagattaag cggatgcagg acgtcattag caaccccaaa cgactggatg
     8281 gagttcccgg gcatatgaat gctgtgcata tccgatttga cagcaactcc cccgtgatgg
     8341 cagatgtgca ggaagaaggc aagccgcagc tgtggaacca taaacaaaat gatgcgttag
     8401 gtctttatct tgatctcctt atacaagcaa tcgacactgg tacaattaac gctgaagatt
     8461 ggcaaaaagg tgaccgatta aaatcggttg cgctgctgat tgcttatctg gataaagcca
     8521 atttctatgt tatggaagat tccggagcat gggaagaaga tgcccgatta aacacatctt
     8581 ccgttgcact ggtgacctct ggactggaac gtttgtcgaa cttactctcg aagaaagact
     8641 ctgtttttgt ctctgattta ctacgcgaag ctaaagccaa tgaactggat gaacctcttt
     8701 caacaacgag gttgaaccac ctcatagata aaggttatga aagaataacg ttacagcttg
     8761 atttaggggg agaatcccca ggttatctgg aaaaagataa acattacaga gaagccgatg
     8821 cggctttact taatgttatt tatcctgcaa atcttgcaaa gataaacacc agaagaaaag
     8881 agcaggttct aaaaatagtt aaaaaactag caggtccgta tggtataaaa agatacgaga
     8941 aggataatta tcagtcggca aatttttggt tcaatgatat taaaaccgat accgatcaaa
     9001 attcacatgc gaaaag
//
LOCUS       DAKISA010000145         9013 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.146, whole genome shotgun sequence.
ACCESSION   DAKISA010000145 DAKISA010000000
VERSION     DAKISA010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9013
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.146"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..280)
                     /locus_tag="OVW45_003852"
     CDS             complement(<1..280)
                     /locus_tag="OVW45_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCV5528189.1"
                     /translation="MHDFENSFDAALAGVDRTIVEVMGLCAQFTSGAQCGSEVQGVFD
                     DPESLGFAGSGVRIEGSSPSLFVRTDTVRAVRRGDTLTINGETFWVDRV"
     gene            complement(273..641)
                     /locus_tag="OVW45_003853"
     CDS             complement(273..641)
                     /locus_tag="OVW45_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528190.1"
                     /translation="MATKEENQKRLRELAGLLGREADMSGSAADIAQRVAEWEEEVCA
                     SENEIADVDDTVHEQACRHTGEDDVGILERIRLLKCFYLCGVDDETGEPVEHVDAGRV
                     ILMPPSVAKDMVRSGMAVYA"
     gene            complement(693..1721)
                     /locus_tag="OVW45_003854"
     CDS             complement(693..1721)
                     /locus_tag="OVW45_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCV5528191.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGDQFEKIEVDFGRSTKN
                     NIIQGSGKEWSKQDRDTFDPTYDIDLFCDQASGLVNIAIMDGTVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPEGTLVLG
                     NTAAEGIRCYGAIKDAQALSEGVVASSRYPKHWLTVGDPSCEFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            complement(1779..2126)
                     /locus_tag="OVW45_003855"
     CDS             complement(1779..2126)
                     /locus_tag="OVW45_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCV5528192.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EATGKLVVWDGQKAGSAVGILVLPLEGTETVLTYYKSGTFATEAIRWPDSVDEHKKAN
                     AFAGTALSHAALP"
     gene            complement(2163..3668)
                     /locus_tag="OVW45_003856"
     CDS             complement(2163..3668)
                     /locus_tag="OVW45_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCV5528193.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYWVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYAGAHKVDGN
                     QFEALPAEVRQDMQQRIDAAHRMFAEKVAMYTGLSVDAVTGTEAAVFEGQSGIEAGLA
                     DELINASDAISVMAAALNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSIEQARAILAAAAPQQPVASAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAAAPLADAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AGSGKAEPVSASTTALFEHFMANHSPAAVRGGVSQTSADGDADVKMLMAMP"
     gene            complement(3658..5250)
                     /locus_tag="OVW45_003857"
     CDS             complement(3658..5250)
                     /locus_tag="OVW45_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCV5528194.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPSQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMFGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTETTQ
                     LFRTRFKAVSPKRVDTPGHGIGNRFLRAGVEVDRYGRAVAYHICEDDFPRSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYA
                     ATIESELDTEKAFEYIAGAPQGQKDNPLINILEKFSSWYDTNHVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIAARLATQMFSCWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEVQESVMRIEAGLSTYEKELALMGEDYQDIFRQQVRESAEREKAGLSRPVWIA
                     QAYQQQIAESRRPEEETTPRET"
     gene            complement(5247..5453)
                     /locus_tag="OVW45_003858"
     CDS             complement(5247..5453)
                     /locus_tag="OVW45_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCV5528195.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAESVLGTTRRRRRPLGVRL"
     gene            complement(5437..7365)
                     /locus_tag="OVW45_003859"
     CDS             complement(5437..7365)
                     /locus_tag="OVW45_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCV5528196.1"
                     /translation="MNISEQQLNNMMSAVTTALQPLIRALPVTPVEWADQNYYLPKES
                     SYGEGEWKTLPFQVAIMNCMGNDQVRTVNLIKSARVGYTKMLLGVVGYFIEHKSRNSL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKDLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GTCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDESTPFGLKWEKDSPESVFYLCEH
                     HGCVIHQSELDQSNGRWICENTGMWTRDGLTFFSAAGNEIPPPRSITFHIWTAYSPFT
                     TWVQIVYDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVHYTAAVPAR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPADEATSYAIRFPDEPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAKSREEETTRP
                     PLKELAAKLSGGVNGYSR"
     gene            complement(7337..7846)
                     /locus_tag="OVW45_003860"
     CDS             complement(7337..7846)
                     /locus_tag="OVW45_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCV5528197.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPISNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDSEDDYEEKLLIARWELTAEQAVTQQLKNEVSKGKLIDTGFCI
                     FALSKLAMALSSTLDSIPLSMQRQFPDLTPRHLDHLKTLIAKGANQCARAGDKLPDLL
                     DEYIRATTE"
     gene            8218..8403
                     /locus_tag="OVW45_003861"
     CDS             8218..8403
                     /locus_tag="OVW45_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3950 domain-containing protein"
                     /protein_id="HCV5528198.1"
                     /translation="MSSKNRTRRTTTRNIRFPNQMIEQINIALDLKGSGNFSAWVIEA
                     CRRRLSTESSGMNYIIK"
     gene            complement(8536..8676)
                     /locus_tag="OVW45_003862"
     CDS             complement(8536..8676)
                     /locus_tag="OVW45_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528199.1"
                     /translation="MDFMLTVSGVVILSIAYTADKYGCHLLSRIGAYCSLMLIFSSLF
                     FE"
BASE COUNT         2135 a         2725 c         2057 g         2096 t
ORIGIN      
        1 aaacacgatc cacccagaat gtctcaccat taatggtcag cgtgtcacca cgccgcacgg
       61 cacgaaccgt atccgtccgc acaaataatg acgggctgct tccttcaata cggaccccgc
      121 taccggcaaa ccccagcgac tccggatcgt caaaaacccc ctgaacttcg ctgccacact
      181 gtgcccccga ggtgaactgc gcacagagcc ccatcacttc aacaatcgtt ctgtccaccc
      241 cggcaagggc agcatcaaag gaattctcaa aatcatgcat aaacggccat tccgctcctg
      301 accatgtctt ttgccactga ggggggcatc agaattactc tgccagcatc aacatgctca
      361 acaggctcac ctgtttcatc gtcaacaccg cacaggtaaa aacacttcag aagcctgata
      421 cgttccagaa taccaacgtc atcctcaccg gtgtgcctgc atgcctgctc atgaacggta
      481 tcatcaacat ctgcgatttc attttccgag gcgcaaacct cctcttccca ctctgccaca
      541 cgctgtgcga tatccgcagc actccccgac atatccgcct cgcgccccag cagaccagcc
      601 agttcacgaa gacgtttctg attttcttct tttgttgcca tatccagctc cctgtgaaaa
      661 aaacacgggg gcatttcgcc cccgctcacg gattatttca cctgtaccac cacaaactca
      721 tccggatccg gcagcaccat cagcggcgcg gactgcgtca tggtgaattc acaggacgga
      781 tcgcccacgg tcagccagtg tttcgggtaa cgggaagaag ccaccactcc ttcagacaac
      841 gcctgtgcat ccttaatggc tccatagcaa cgaatcccct ctgccgccgt attccccagc
      901 accagcgtgc cctccggcag ataacgtttt tcggtaccgt cctctgccac ataagacgtt
      961 ttcgctacca caatagccag atcgccgtaa taccccttga aggataccac tgcgcccaga
     1021 tctttcactg ccgtttcgag ttgtgaattt gaaccgcgac gggtatccag tttttcgcgg
     1081 aaaagcttaa agccattcag cagacgccag acggtaccgt ccataatggc aatattcaca
     1141 agaccactgg cctgatcaca gaaaaggtcg atatcatatg tcgggtcgaa cgtgtcacga
     1201 tcctgttttg accactcctt accgctaccc tgtatgatgt tattcttcgt cgatcggcca
     1261 aaatcgactt caattttctc gaactggtct ccttccatcg tatatttgcc atacagcacg
     1321 gcattcaccg cctgcatttc ttccacctgc acaatagcgt gctcttcctg tttgaggtta
     1381 tcagtgatga tacgcagacg acggtaggcc gggtcgttca gctgagccgg atcttcaccg
     1441 ggaaggcgtt caaccgcctg ctggtaatta aattcgtgtt tcggcttgac gtagcccgga
     1501 cgtaacacgc gggtttcacc cccacgatga cgcagcactt ttccttcaac gaccggggag
     1561 acataggccg ccaccggcgt ttttccggta attttgtcca gcatcacctc ttcggtgtgg
     1621 aaattcaccg tacggcggaa aaacagttcc agaaacagcg cacgaaattt aactttttgt
     1681 tcggtataac cgagtaactg gcgggtcgta aacaatccca taaatcagtt cctttcattc
     1741 agaaatcagt caggccacca tggtggcctg ataacgtgtt acggcagagc cgcgtgactc
     1801 agggctgtgc cggcaaaggc atttgccttt ttgtgttcat ccacactgtc aggccagcgg
     1861 attgcctccg tcgcaaaggt ccccgacttg taataggtca gcaccgtctc tgtgccttca
     1921 agcggcagta ccagtatgcc aaccgcacta ccggctttct gtccatccca gaccaccagt
     1981 ttcccggtgg cttcatccag catcaggggc gtcagagccg gtgttgcaga agaaatcccg
     2041 ctgctgcctg tggcggtatg agccggatca ttaccagcaa aaatacgtac ttccgcacgc
     2101 tgttcagtga tggttttcgt caccattttg ttaaaacctc atattgatgg tcagcactga
     2161 cttcatggca tggccatgag cattttcacg tccgcatcac cgtctgctga cgtctgtgac
     2221 acgccacccc gcaccgctgc cggtgaatga ttcgccatga aatgttcaaa cagggcggtt
     2281 gtggatgcag agaccggttc ggccttacct gatcccgcag ccagcacagc ccgggcgttc
     2341 tccacggtca ttcccgggca ggccgccagt ttttcagcct gcgcttctgc cccttttgcc
     2401 tcatccaggg ccatgatctg atcacgaagt gagggcccgg catccgccag tggtgcagcc
     2461 gccaggatcg ggcgtgcttt ttccaccgtc atctccggca tcgccgccag cgttgccgcc
     2521 agttgttcac gacctttcgc ttcttcacac gccataatgc gatcggcttc actctgcgcg
     2581 gatgccaccg gctgctgtgg tgccgccgcg gccagaatcg cccgggcctg ttcaatgctc
     2641 atgccctgtt gtcctgccag catcgtggca agctgttcac gtcctttcgc ttcctgacac
     2701 gtcaggatcc ccatcactcg ctggttctcc tgcgcggcag cttccgttgc agttaattgc
     2761 ggcatagtgc ctcctgtatc atgagtgttc agcgccgcag ccatcacgct gatggcatcc
     2821 gacgcattga ttaattcatc cgccagcccg gcctcaatgc cggactgacc ttcaaaaacg
     2881 gcggcctctg ttcccgtgac cgcatccaca gacaacccgg tatacatcgc cactttttcg
     2941 gcaaacatcc ggtgcgccgc atcaatccgc tgctgcatat cctggcgaac ctctgccggc
     3001 aacgcttcaa actgattgcc atccaccttg tgcgccccgg cataaatcag cgtgatatcc
     3061 acaccggcct gcgccagatg accggcatag ctgacatggc tcatcatcac gccaatggag
     3121 ccgatacggg atgtctgggt aaccagccgt cgggagcagg ccgacgccag cagcatggct
     3181 gcagaacagg ccgtgtcatt gcacagtgcc cagaccggct tctgctgacg gaggcggtaa
     3241 atcatgtcag cgcagtcaaa cgcgccggcg gcctgcccgc ccggactgtc aatgtccagc
     3301 agtacgcccc gcacctggct atctgccatt gcctgctgaa gacaggcgac aatgccgtca
     3361 tagcctgtca ttccggaaaa tggccgcata ccgcccagcc ggtgcaccag cgtgccggtc
     3421 accggcagta cagcaatacc gttcaccacc cagtaaacac gggccggtcg tttacctccg
     3481 gccatgtact cgtccgtttc agccagcatt ccgggagcat caagctgtac ctgttgttgt
     3541 ggtaccgaaa gacttgctgc ccccatctcg cgcccgagcg cgcaaaagaa aacccgcgca
     3601 taggcgggct ccagaagcag cggttcattg aatgctgcgg caataatgtg tgaaagatta
     3661 cgtctcacgt ggtgttgtct cctcttccgg cctgcgactc tccgctatct gctgctgata
     3721 cgcctgcgct atccacaccg ggcgtgagag tccggctttt tcccgctctg cagattccct
     3781 gacctgctgg cggaaaatgt cctgataatc ctcgcccatc agcgccagct ctttctcata
     3841 cgtgctcagt ccggcctcaa tgcgcatcac tgattcctgg acttccttga gcccgtcaat
     3901 ggccattctt ccggcaccaa tccactctgc ccgtgaccag gctgatcgcg cctgataaaa
     3961 atcaaaacga gcccgtggcg gacgaataat cccccgaaga agtgcctctt ccagccagca
     4021 ggaaaacatc tgcgtggcca gtcgggccgc aataaatttt cgccgcccca taaaatagcg
     4081 ccacgactca ttggcggagg cgcgggcact tgagtaactg accttcgagt aatcacggga
     4141 caactgttcg taggaaacgc caagaccggc ggcgatatac cgcagcagcg cctgttcaag
     4201 cgccgaaaat ccattgtctg aatcctgcgc tgtctgcagt ttcagatcat caccagggaa
     4261 aaggtgcgga attttgacac cgcccagcgt cacgtgattc gtgtcatacc agctggagaa
     4321 cttctccaga atattaataa gcggattatc cttctgcccc tgcggcgcac cggcgatata
     4381 ttcaaaggcc ttttcggtat ccagttcact ttcaatcgtc gctgcataca tcgccttcac
     4441 tatggccgac tgaagctgtg ttgcctgcag ggaatcgagc atcttcagcc gttccatgac
     4501 gctgtaaaac tgattggccc cacgggtctg cccgtcctcc accggctcga aaatatgcag
     4561 catggccgga cgcccggtgg gtagttcacg cgggatccgt tcccatcgtc cactcccgga
     4621 gcgaggaaaa tcatcctcac agatatggta cgcaacggca cggccatatc gatcgacctc
     4681 cacaccggcc cgcagaaaac ggttcccgat accgtgtcct ggcgtgtcca cccgtttcgg
     4741 actcacggct ttaaaacgcg tacggaataa ctgcgtggtt tccgtatccc agaccggctg
     4801 cacaaagatt tcgccgttaa acgcatgaac gcccacacct tcacggataa attccgtaaa
     4861 cgtgcgtttc ccttccacgt cgatctcacc aaacatccct tctgcgtatt ctgaccaggc
     4921 cgcctccacc tcatcgacaa aactttttgc cgcggtctcc cgcatcccca gccagcgcca
     4981 gttcggacgg tagctgataa gaaacatatg cccgacaatg tgatccttat gcagggccac
     5041 cgcattggcc gctatcccgt tattgcgcac cagatcatct gcccgggcat tccccagacg
     5101 caacgcgggc agcagggccg catcggcact ctgcgagggt ggcaaccact ccgccatttg
     5161 cccgccaaat cctgcgccgc ccccgttgta gctgaggctc tcccgaagcg gaacgccgtt
     5221 cacatcaatc aggacaggcg ttcgtttcat aacctcactc ccagcggacg acggcgacgc
     5281 cgggttgtcc ccagtaccga ctccgcatca ttgatcgccc ggttaagctc atccagagaa
     5341 gccgccgtat attcaattct gcgaccatct ttctggacag acaccacccg tttaccggtt
     5401 aataaatcaa ggcgcgcctg acgcagcgcc tgcagttcag cgactgtaac cattcactcc
     5461 tccggacagc ttcgctgcca gttctttaag gggtggccgg gtcgtctctt cttcccggga
     5521 ttttgccagt acagccagat caagctgcca gcgttgcacg gacacacgta atgccgcgta
     5581 ggcatacacc aggcagtcca gcgcttcgtt acgccgcttt ttgttatccc acagcagacg
     5641 catctttcct ttttcccact tctccacaag ctcttccgcc accagttgct gcgcctctgt
     5701 ctgcgaaaaa atctccggct catcaggaaa acggatggca tacgacgtgg cttcatccgc
     5761 aggcgtggga tcggctttca tacgggcata gagaatttct tttgcggtgt ccgtccccac
     5821 ttcgcacaga tacacgcccc gctgattgcg ggtttttggc atggtgatca ccggcttgcc
     5881 ataaacagat gcacctttta ccggcagcac ccggaaaaca ccgtgttttt ttgacctctg
     5941 ataaacgatt tcgccatcga tccccccggt gtcccagcag acacgggaaa tggtcatctc
     6001 ggtgccatcc gcatggcggt attttttgtt gatcgccgca tccacacgta acagcgtctc
     6061 ttcctcatca ggacgcccca taatgatgat tttatccacc agaaaagctt cctctcccgg
     6121 tgcccatccc cagacataca tctcaaaacg gtttcgctgc gagtcaatgc ccgccgtcag
     6181 ataaaccacc cgggcaggta ccgccgccgt ataatgcacg accttatcca tcagcacctg
     6241 gtgatcaagt ttttcgccca cggcctcttc ccaggtctcg cccagcgtgg tgttcacaaa
     6301 ggttttcacg ccgttgggat ctttcagtgc atccagccag tcatagacta tctgtaccca
     6361 ggtggtaaac ggactgtacg ccgtccagat atggaacgtg atggagcgcg gcggcggaat
     6421 ttcattaccc gcagcgctga aaaacgtcag accgtcacgg gtccacatgc ccgtgttttc
     6481 acagatccac cgcccgttgc tctggtccag ttcagactga tggatcacgc agccatgatg
     6541 ttcacagagg tagaaaacac tttcgggact gtccttctcc catttaagcc caaaaggcgt
     6601 ggactcatcg ccaaatttca gatactgctc ctccccacag tgtgggcagg gcacataaaa
     6661 acgcataaaa tgcgccgact cgttggccgc tttttcgatc tggcaggtgc ctttgatttt
     6721 aggcgtcgag ccgcgaatgg atttgggcca tacagagccc tcaatacgtt tatccccaag
     6781 cagggttggc gacccctctt tttcgacatc cggttcgaac gaggaaagtt cgtcatagca
     6841 gaccacgtcc acggattttt cacggtagtt tttagcggca gccccgccca ggcaccagaa
     6901 gcccacgccc gatgaaaaac gtttcagcgt gagggtatta tcacgatgtt tacgacccag
     6961 ccatggggaa aggtctttca ggcatggcac atcccgaatc gtcgcctcca cgtgagactt
     7021 cataaaatct tcagcggcag aatccgtggg ctgaaaaagc agactgtttc gtgatttatg
     7081 ctcaataaaa tacccgacca cccccagcaa catctttgta tagccaacac gggcagattt
     7141 aatcaggtta accgtgcgaa cctggtcgtt acccatacag ttcataatgg cgacctggaa
     7201 cggcagcgtt ttccattctc cctcaccata tgaagattct ttaggcagat aataattttg
     7261 atcagcccat tcaactggcg tcaccggcaa tgcccttatc aggggctgta atgctgttgt
     7321 gacagcactc atcatattat tcagttgttg ctctgatata ttcatcgagt aaatccggta
     7381 atttatcccc tgcccgcgca cactgatttg cccccttcgc aataagggtt ttcagatggt
     7441 caagatggcg tggtgttaaa tcaggaaact gtcgttgcat ggataaaggg atggaatcaa
     7501 gcgtactgga taacgccatt gccagcttgc tgagggcaaa aatacagaac ccggtgtcaa
     7561 taagttttcc ttttgacacc tcatttttta actgctgtgt aacagcctgt tctgctgtca
     7621 gttcccatct ggcaataagc aatttttcct catagtcgtc ttcgctatcg ccatcaggca
     7681 catcgttttt acttcttctc agatacgata tgtaaaaatc gcgccaggca tccagatcca
     7741 gttgccctcg cttattcgat atcggggcac ccggcaattt ctgcaatctg cgaagctggc
     7801 gatcggtcag acttaaatgc ctggcaactt cagtctgcgt agccactcct cacctcgcaa
     7861 aaactctcac ctcacaatca caacaaaacc ggtcatgtcc ggttcacaga agacctgttt
     7921 ttatattttt catatagtta acttgaagat aaaccggaca tggatccagg aaaattttca
     7981 taaatagcga aaacccgcga ggtcgccgcc ccgtaaccgg tcggatcgcc ggaaaggact
     8041 cacgaaatga taattattat catctatata aggtttatca caacatgtgt gtacgccatc
     8101 aaaccacgag aaataatcaa ttatgacgca ggtatcgtat taattgatct gcatcaaatt
     8161 aacgtaaaag caacttcaga taatacaaat cagcaacact gaatatgggg aaacattatg
     8221 tcatcaaaga acagaacccg cagaacaaca acccgcaaca tccgatttcc aaaccagatg
     8281 attgaacaaa ttaacatcgc tcttgatctg aaaggttcag gaaacttttc agcgtgggtt
     8341 attgaagcct gcagaagaag gctgtcaaca gagagttcgg gtatgaatta cataattaag
     8401 taacatggtg ttcacagaac acgcagttac cggacacatc agttttccat tcgctccctg
     8461 gcagtacagg cttcccctct gacgggatag cctgaaaaaa taacacagaa aattatttgt
     8521 tataattaat ataacttact caaaaaaaag cgacgagaaa atcagcatca acgaacaata
     8581 agcgccaata cgtgataaca aatggcagcc atatttatct gcagtataag caatggacag
     8641 gataaccaca ccagaaaccg tcagcataaa atccatttga acttccccgg acaaaatcga
     8701 ctcatctaaa gatttacagc tctttttatt attaatatgt taaaagcaaa ataaacagat
     8761 gttcaataac acgaatacaa aaacgtgctg aaattcaatg aatccatttc tgtgtcatca
     8821 attaatagtg ataaacatcc ggcttcttcc accatcgcac cggacaggcg actatgaggg
     8881 gacaacgccg cgctccgtta acgcggtaaa ccccggtgtg tatcgttttt gattatcccc
     8941 gcacactcgc gcagaggagt ctccctgtcg ggctgcggtc tctgttaatg agggaataca
     9001 gcgacgatac ggc
//
LOCUS       DAKISA010000146         8966 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.147, whole genome shotgun sequence.
ACCESSION   DAKISA010000146 DAKISA010000000
VERSION     DAKISA010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8966
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.147"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..96
                     /locus_tag="OVW45_003863"
     CDS             <1..96
                     /locus_tag="OVW45_003863"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCV5528200.1"
                     /translation="PAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(152..487)
                     /locus_tag="OVW45_003864"
     CDS             complement(152..487)
                     /locus_tag="OVW45_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCV5528201.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(491..835)
                     /locus_tag="OVW45_003865"
     CDS             complement(491..835)
                     /locus_tag="OVW45_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCV5528202.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            complement(837..1010)
                     /locus_tag="OVW45_003866"
     CDS             complement(837..1010)
                     /locus_tag="OVW45_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528203.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            1111..1296
                     /locus_tag="OVW45_003867"
     CDS             1111..1296
                     /locus_tag="OVW45_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528204.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            1257..1376
                     /locus_tag="OVW45_003868"
     CDS             1257..1376
                     /locus_tag="OVW45_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCV5528205.1"
                     /translation="MPLSYAGYHHFIMISLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(1855..2121)
                     /gene="minE"
                     /locus_tag="OVW45_003869"
     CDS             complement(1855..2121)
                     /gene="minE"
                     /locus_tag="OVW45_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCV5528206.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2125..2937)
                     /gene="minD"
                     /locus_tag="OVW45_003870"
     CDS             complement(2125..2937)
                     /gene="minD"
                     /locus_tag="OVW45_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCV5528207.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2961..3656)
                     /gene="minC"
                     /locus_tag="OVW45_003871"
     CDS             complement(2961..3656)
                     /gene="minC"
                     /locus_tag="OVW45_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCV5528208.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            4176..4544
                     /locus_tag="OVW45_003872"
     CDS             4176..4544
                     /locus_tag="OVW45_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HCV5528209.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFVNGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(4652..5053)
                     /locus_tag="OVW45_003873"
     CDS             complement(4652..5053)
                     /locus_tag="OVW45_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HCV5528210.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            5295..5588
                     /locus_tag="OVW45_003874"
     CDS             5295..5588
                     /locus_tag="OVW45_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCV5528211.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            5660..6319
                     /locus_tag="OVW45_003875"
     CDS             5660..6319
                     /locus_tag="OVW45_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCV5528212.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            6411..6857
                     /locus_tag="OVW45_003876"
     CDS             6411..6857
                     /locus_tag="OVW45_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCV5528213.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMICAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(7064..7698)
                     /locus_tag="OVW45_003877"
                     /pseudo
     CDS             complement(7064..7698)
                     /locus_tag="OVW45_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin E"
     gene            8071..8490
                     /locus_tag="OVW45_003878"
     CDS             8071..8490
                     /locus_tag="OVW45_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCV5528214.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            8490..>8966
                     /gene="umuC"
                     /locus_tag="OVW45_003879"
     CDS             8490..>8966
                     /gene="umuC"
                     /locus_tag="OVW45_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCV5528215.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQ"
BASE COUNT         2305 a         1986 c         2092 g         2583 t
ORIGIN      
        1 ccggcggcca gtgtctgggg taatgtgggc gtgcagctgg gtgataatgg ctacaatgac
       61 accgcagtga tggtgggcct gaaatataag ttctgatccc cgttagctaa aataccgcgt
      121 cgaattcatc gacgcggtaa catattaaat attatttttt gccgatagca cgcatggtgt
      181 catcaattgc cgtgatcaac agcatttgcg ggtctttagc gcaaacctga ttcagttttt
      241 ctaccacttt ggcgctcagt tccggagatt gctcaatttt taaatcacgg ctggcaacgc
      301 tggcattacc catcaccgca acaatttctg caacctgtgc gctgtcagtt tttgccattt
      361 cgttggcttc tgcgcaagta atataggttt ctgacggcaa accgttttta atattgtagt
      421 cctgcgccca ggtcattggt gcgaaaacaa acaggcccgc cagtaaagca aattttttca
      481 tcatcattcc ttatttcatt ttacccagaa ttgcaccacc cgtaccgcca atcacggcac
      541 ctttaatcgc cccttcgagg ccattgccgg tcagaacgcc agtgacagca ccaacggcgg
      601 cacccacttt tgcaccttta cgtgcatttt taccgtcgcg gcctttttct gttactgcac
      661 caacaccagc gccaacagct gcacctttca gtacgccatt aacaccattg ccagtaagta
      721 atccaacgcc tgcgcctagc aatgcacctt tcgtggtgcg gttcatatcc gccatcgctg
      781 gcgtggagca gaacaatgct gagataagcc cgaaggcaag tatttttttc ttcaacttag
      841 atgtccggta ttaagtaagt tgcacacaca ataatttcgt cttcaattaa gatctgctta
      901 actaaagaac gctcgctatt attcagataa ttcaaaatga gcgtggctgt gatgatagga
      961 attatgtttt ttacgtgaat gagaataatc ttaaatgagg aataactcat tgattggcaa
     1021 tatttttatt caagaagtgt cattgactgt taacgcaatg ttgtaaaggt aagataatct
     1081 gatttatcaa tattattgtg tgatttttat gtgagcagaa gatattcatc agcaacgatt
     1141 acattagtca ttttattttg ccgacggcct cattgtcgaa agataagcgt acgacagtat
     1201 tatctgaaaa gagtgatttt ttatccaact acacttcagc gcactgcgtt taaaaaatgc
     1261 ctctttctta tgcgggatat catcatttca tcatgatatc tttggtgagc ggtgaacaca
     1321 atacacctgc gctgtctctt caggatgaat cccctggtct ggtgcctgcg ggctgatgtt
     1381 gcagcagagc ttaggtcact tagacgctac tatcatttat ccaatggcat ggaatcgaaa
     1441 tcagtcgata cccgcagtat atatcgtgaa ctggtcgttc tgaccatcaa cgattaaact
     1501 gcgcttcggc gcagttttcg tttacagggg gctaaaaggg aaaattctgg ggcaaaaaaa
     1561 gcccgccagt tacggcggga aacctcatcc tatgggagaa caatgaataa tgaaattgcg
     1621 gggttatcat cccccagtgt atccacacta acaataaggt tatttactca gccaggcata
     1681 aacattatgt tctgtgcgtg ggaacagcct taaggtgtaa agggggaggt ggaaatagca
     1741 atgaggagta tcagcaagaa tactcgccgc tttaccacaa cgtggatgag agggatgaaa
     1801 aactcaaggc agaaatgact ctgccttgaa gataaatgcg tttttacagc gggcttattt
     1861 cagctcttct gcttccggta aggtcacgtt cagctcaaga atagaaatat cgccatcttt
     1921 ttgctcaagc tgtacggtta ccatctcagg atcaatttgt acgtatttac aaatgacctc
     1981 aagaatatct ttacgcaact gcggcagata atgcggttct gcatcgctgc gacggcgttc
     2041 agcaacaata atctgcagcc gttcttttgc aatgttggct gtgtttttct tccgcgagag
     2101 aaagaaatcg agtaatgcca taacttatcc tccgaacaag cgtttgagga agcctttctt
     2161 ctcttcttca atgaagcgga aaggacgttc ttctcccaac agacgttcta cggtatctgc
     2221 gtaggcttta cccgcatcgg cgttaatgtc gagaatgacc ggttcaccct ggttagaggc
     2281 gcgcaatact gattgatcct ctgggatcac gccgacgagt ttgatgcgca ggatctccag
     2341 cacatcttcc atgctcagca tgtcacctct gcttacgcgg cctgggttat agcgcgttaa
     2401 cagcaggtgc tctttaatag gctcttcgcc attttctgcg cggcgtgatt tcgacgccag
     2461 aatgcctaaa atacggtcag agtcgcgtac tgaggagact tccgggttgg tggtaataat
     2521 ggcttcgtct gcaaaataga gtgccattaa cgcaccggtt tcaatccctg ccggggagtc
     2581 acaaacgata aattcaaaat ccatcgcttt cagatcatca agaactttgg cgaccccttc
     2641 acgggtgagg gcatctttat cgcgtgtttg cgatgccgga agaatataga gattttcagt
     2701 acgcttatct ttaattaacg cctgatttag cgttgcatcg ccctgaatga cgttgacgaa
     2761 atcgtaaacg acccggcgtt cgcaacccat aatcaggtcg agattacgca ggccgatatc
     2821 aaaatctatc acgacagttt tctttccctt ctgggccaaa ccagtggcga tggccgcgct
     2881 ggaggttgtc ttaccaaccc cccctttgcc cgaagtaaca acaataatgc gtgccataga
     2941 aattccttgt taaaaaggga tcaatttaac ggttgaacgg tcaaagcgtt ttcgactaac
     3001 tgcagtcgcg ccgctttgcc ataaaattct gctgggattt gatcactcag ccagtattca
     3061 cctgcgatgg acaccaattc cgccatcagg ttcgtacaaa atatttgcgt ttcccggtca
     3121 ccacttgccc ctgccagcgc acgaccgcgc atcatgccat agacatgaat gttcccatcg
     3181 gcaatcaatt cggccccagc gctaacgtgg cttgtaacaa tcagatcaca ttgtggagca
     3241 taaatacgct gaccggaacg caccggggta tctattaaac gcgtttttgt gaccggcgtg
     3301 gtattttgcg ctggagcctg tggtgcggga gctggacgtg gcgctttttc ctttccttcc
     3361 gtcaggatag gcagccccat tttttcaatt tcggctttaa gttgcgcgtc tttgcagcca
     3421 cttacgccaa taacccgcaa accggttgcc gaaaccgcct tatgcatcgc tgaccagttt
     3481 accgggtctt ccagtgcact gacgttgagt acaacggggg catgttttaa aaatgcgggg
     3541 gcctgagcga ttttgtcttc cagcgcctga tggataacct taggttctgc ctcatgcaga
     3601 tgaaccacag ataaagtgaa gctactgcct ttaagctcga ttggcgtgtt tgacatcctg
     3661 gccttactca attagctatt aatcatcgcc agcgcgcgat gatgttccga agactataag
     3721 gcatgttata gtctggatta tattgaggca agtcaccctc ccatttattc agagtaaaag
     3781 tctattctgt gataaatggc gctgattcat agcttaaaaa atacccttgt caatcaaccc
     3841 attgccgccg tacttttgat tcttcttatt tacgcttcct ttttccgcac cctaatcgtg
     3901 acagcatctc cagacctgtt taaatatcca gcattgaggc aagtgtattt tttcaccacg
     3961 ccaggctttc ttaactttcc attttttgca gcatcattaa agaacagata cccgttatct
     4021 taatatccgc cacagaattg ctctatcata gaaaccgcag aagacgtctg atacttctgt
     4081 caggtcatcg taactcgtcc aggtcagcgg ttttgattac ggattgatga cgaaatgagc
     4141 ataagtgttg tacggttgga ttatcaggat gtgctatgaa tatgatgcgt attatttata
     4201 tcggattgtc aggtgtgggg atgatgttct catcaatggc gtctggacat gacgctggcg
     4261 gacttcaatc tccggcgtgc ggcgttgttt gcgacccgta tatttgtgtg aactcagatg
     4321 gcatttcccc agagttaaca aggaaatatc tcggcgaaaa agccgctgaa aacttacaat
     4381 cattacaagg ctacgatccc agcgaattta cattcgttaa cggtgtattt tgcgatgtta
     4441 aagaaaaatt atgtcgtgat gatcgttatt ttggtgtaga tggtaagcgg agtggaaaaa
     4501 tcaatcaaac caccacaaag atgttattta tgtgtcgtga gtgatgcctt gtttacatta
     4561 tgttctctgc tattcgtcat taacctcttg agcgacagag aggagtgggg cgttaacgtc
     4621 tatgatgggg atagtccccg tcggttgaca tttacttaat ttgaatatcg acgttatatt
     4681 ttttagtttt gtttttacgg gcgtcgtttc gagtttgcag cactcgcagc tcatatttgc
     4741 cagaagcagg gagtgagtat tgtccgtgac tatcgagttc tggggaatat ctggataaat
     4801 caacagaatc atcgattcct ggcccgaaga gataagtatc agcgccttca ttagagatac
     4861 tcacatgcac tttctgacct tttttggcat agaaggtata tgtatcgtaa tcgtatccct
     4921 ttatttcgcc ggagtactga gcgctgctat ggcctttgct gaactcaaca ttgacatttt
     4981 tacccgcagc aaagctcgtg gaggttagca gtgcaagcag caatacagcc ttactgatgc
     5041 tcttgatttt cattggtagt ctcctgttca gaatgagtac gatgaactgt tataatataa
     5101 caatccctta cgggagaatt ccagtggctt ttctgcgttc ggtaaaatcc cggcgcggca
     5161 ttttttgccg gggggattac ttataaaggg gcatggactc aacaaaccgc agaaatctac
     5221 agctggttgg cttgtggcgc gggtttcatt gatgttagct tatgccaaaa cctggcatct
     5281 taaagagtaa aagtatgttt tgtgtgattt atcgaagcag caagcgtgac cagacctatt
     5341 tatatgtcga aaaaaaagac gatttttcgc gtgttcctga ggaactgatg aaaggttttg
     5401 gtcagcctca gttagcgatg attctgccgt tggatgggcg gaagaaactg gtcaatgctg
     5461 atattgaaaa ggttaaactg gcattaaccg agcaaggtta ctatttgcag ttaccgccac
     5521 cacctgaaga tttgctgaag caacatcttt ccgtcatggg gcagaaaaca gacgacacta
     5581 acaaataacc gatatccggc ggtggcatta tctttgtcgg cgcgggtttt catatccacg
     5641 ataaggtgag gggaacgtta tgtatcaaca tcacaactgg caaggtgcgc tgctggatta
     5701 tccggtgagt aaagtagtct gtgttggcag taactatgcc aaacatatta aagagatggg
     5761 cagcgcagtg cccgaagagc cagtgctgtt tattaaacca gaaacggcac tgtgcgatct
     5821 gcggcagcca ttggcgatcc catccgattt cggttcagtt catcatgaag tcgaactggc
     5881 ggtgttgatt ggcgcgacgc tgcgtcaggc aacggaagag catgtccgca aagccattgc
     5941 cggttacggc gtggcgcttg atctgacatt gcgtgatgtt cagggaaaaa tgaagaaagc
     6001 cgggcagccg tgggaaaagg ctaaagcgtt tgataactct tgtccgcttt ccgggtttat
     6061 ccccgcggcg gaatttaccg gcgatccaca aaatacaacg ctgggcctga gcgtaaacgg
     6121 cgaacaacgc cagcaaggta cgactgcgga catgatccat aaaatcgttc cgttgatcgc
     6181 ttatatgagc aagtttttta ccctcaaggc cggtgacgtt gtgttgacag gcacgcctga
     6241 tggcgtcggc ccgttgcaaa gcggtgatga gctgacagtc actttcgatg ggcattcttt
     6301 gacaactcgc gttttgtaat actttttgcc gcctgaaagc ggcggcaaaa cttgcatcgc
     6361 tgtgccagac tggttataag gtgcgtttta acgtaatggc ggaacacctg atgagcgatg
     6421 tacctttctg gcaaagtaaa accctggacg aaatgagcga tgcggaatgg gagtcgttgt
     6481 gtgatggttg cggtcagtgt tgcctgcata aactgatgga tgaagacacc gacgaaatct
     6541 acttcactaa cgtcgcctgt cgccagctca atattaaaac ttgtcaatgt cggaactacg
     6601 aacgtcgttt cgagtttgaa cccgactgca ttaaattaac ccgtgaaaat ctgccaacat
     6661 tcgaatggct gccaatgatc tgcgcttatc gtttgctggc ggaaggtaaa gatttacctg
     6721 cgtggcatcc gctacttact ggttcgaaag cggcaatgca tggtgaacgt atctctgtgc
     6781 gtcatatcgc agtgaaagaa tcagaagtga ttgactggca ggatcatatc ttaaataaac
     6841 ctgactgggc gcagtgataa atttaataga tgttgatggg ggctttctta atatacgtct
     6901 ggatgctcgc catttcgtag ccctgttttt gaaagcgctc tgtcataatg agagttaccc
     6961 gataccttat tatcaacagg taactctcaa tctttaaaaa acgtctgtgt ttttaaatgt
     7021 gataaacctt ataccttgag tacatggaga gaataatcgc tgatcagact tcaggtacct
     7081 caaagagtgt ctttttaccg tgtcttttct gatactcatt acaggtgtta atcatttttt
     7141 tggccgcttc ttttagcaaa gaaagcatta aatcatcata atcaacgtag aatctggtgg
     7201 tttcagtttc cgtttttatc tctccgatgg cggctatttc ggtggttaat ttcaatttgg
     7261 cggcatcgat atctttattc gcttgtttaa ccgtgttaga cagggtggta aagaaactct
     7321 gcacagattt taacttgttc ttcaattctg ggatcagttt cccttcaact acgcccgcag
     7381 caatagaata ggaaatgatt aaaccaaatg gaccggcgac gacaccggct gcggcaccgg
     7441 catacgcttc cttcctgatt ttatctacct gtgactggaa atagctgctt ttttctgaaa
     7501 aatcattggt taactggcta tctaacgcca gcagtttccc ggaagcgttg ttgaaactaa
     7561 ctcttttatg gtttcatcaa aggtctgcca ggggatgacc tgatcgagat atttattata
     7621 aagatctaat gctccatctg cggtttcgat tgcgttttta actacttcta ccgttttatc
     7681 tgcaacgatt tcagtcataa tcattcgcct ctttaaatat ataaattgta atgaaactcc
     7741 tgttttacaa ctattaataa attttacttc atctaattca tagttagccg ggcgggatgc
     7801 gtcaatgtct ttatttctat taatatgata aatatcaaac aatgtttaat gtcattatgg
     7861 cgaatgcttc tattctattt ttttgccggg tgatattttt catttctgct ggatgagcgt
     7921 cgtcgccaga aggccacgtg agcacaggat aagagaacga aaaatcagca gtctatgcag
     7981 cgacaaatat tgatagcctg aatcagtatt gatctgctgg caagaacaga ttactgtata
     8041 taaaaacagt ataacttcag gcagattatt atgttgttta tcaagcctgc ggatctccgc
     8101 gaaattgtga cttttccgct atttagcgat cttgttcagt gtggctttcc ttcaccggca
     8161 gcagattacg tcgaacagcg catcgatctg aatcaactgt tgatccagca tcccagcgcg
     8221 acttacttcg tcaaagcaag tggtgattct atgattgatg gtggaattag tgacggtgat
     8281 ttactgattg tcgatagcgc tattaccgcc agccatggtg atattgtcat cgctgctgtt
     8341 gacggcgagt ttacggtgaa aaaattgcaa ctacgcccga cggtacagct tattcccatg
     8401 aacagtgcgt actcgcccat taccatcagt agcgaagata cgctggatgt ctttggtgtg
     8461 gtgatccacg tcgttaaggc gatgcgctga tgtttgctct ctgtgatgta aacgcgtttt
     8521 atgccagctg tgagacggtg tttcgccctg atttatgggg taaaccggtg gttgtgctat
     8581 cgaataatga cggttgcgtt atcgcccgaa acgctgaggc aaaggcgctg ggtgttaaaa
     8641 tgggcgatcc ctggttcaaa caaaaagatc tgtttcgtcg ctgtggtgtg gtttgcttta
     8701 gcagcaatta tgagctttac gcagacatga gcaatcgggt gatgtcgacg ctggaagaac
     8761 tatcgccccg cgtcgagatt tacagtattg atgaggcatt ctgcgatctg acaggtgtgc
     8821 gtaattgtcg cgatctgact gattttggca gagaaattcg cgcaacggtg ctacaacgta
     8881 cccatcttac tgttggtgtg gggatcgccc agaccaaaac gctggctaag cttgccaatc
     8941 atgcggcaaa aaaatggcag cggcag
//
LOCUS       DAKISA010000147         8953 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.148, whole genome shotgun sequence.
ACCESSION   DAKISA010000147 DAKISA010000000
VERSION     DAKISA010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8953
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.148"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            40..708
                     /locus_tag="OVW45_003880"
     CDS             40..708
                     /locus_tag="OVW45_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528216.1"
                     /translation="MSEQIKQDIDLIEILFYLKKKIRVILFIIAICMAMVLLFLYVNK
                     DNIKVSYSLKINQTTPGILVNCDSNNNFACQTTMTEDVIQRITTFFHTSPDVKNREIK
                     LEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVLDEIQIPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(951..1646)
                     /locus_tag="OVW45_003881"
     CDS             complement(951..1646)
                     /locus_tag="OVW45_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCV5528217.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1639..3066)
                     /locus_tag="OVW45_003882"
     CDS             complement(1639..3066)
                     /locus_tag="OVW45_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCV5528218.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3077..3796)
                     /locus_tag="OVW45_003883"
     CDS             complement(3077..3796)
                     /locus_tag="OVW45_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCV5528219.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(4324..5178)
                     /gene="rclR"
                     /locus_tag="OVW45_003884"
     CDS             complement(4324..5178)
                     /gene="rclR"
                     /locus_tag="OVW45_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RclR"
                     /protein_id="HCV5528220.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLQHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            5404..6729
                     /locus_tag="OVW45_003885"
     CDS             5404..6729
                     /locus_tag="OVW45_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HCV5528221.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            6838..7074
                     /locus_tag="OVW45_003886"
     CDS             6838..7074
                     /locus_tag="OVW45_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCV5528222.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            7086..7679
                     /gene="rclC"
                     /locus_tag="OVW45_003887"
     CDS             7086..7679
                     /gene="rclC"
                     /locus_tag="OVW45_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HCV5528223.1"
                     /translation="MEKYLHLLSRGDKIGLSLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAYTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(7839..8708)
                     /locus_tag="OVW45_003888"
     CDS             complement(7839..8708)
                     /locus_tag="OVW45_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCV5528224.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWGQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSVWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
BASE COUNT         2476 a         2132 c         1900 g         2445 t
ORIGIN      
        1 caaccttatc tgcggcagat tttattttct ggatatatta tgagtgaaca aatcaaacag
       61 gatatcgatc tgattgagat tttattttat ctgaagaaaa agattcgtgt tatccttttt
      121 attatagcta tctgtatggc aatggtgctt ttatttctgt atgtcaataa agataatata
      181 aaagtgagtt acagcctaaa aataaaccag acaacaccag gcatacttgt taactgtgat
      241 agcaataata actttgcctg tcagaccaca atgactgaag atgttataca gcgaattact
      301 acattttttc acaccagccc agatgtcaag aacagagaaa taaagctgga atggtcagga
      361 gataagagag atttaccaac tgctgaagcg gaaatatctc gcgtgcaggc gtctattatc
      421 aaatggtatg cgtcagaata ccataatggc aggcaagttc tcgatgagat acaaatacct
      481 tcagcaatta acagtgagct ttatacaaaa atgatatacc tgaccaggaa ctggtcattg
      541 tatccgaacg gtgatggctg tgtaactata agctcaccag aaataaaaaa taaataccct
      601 gctgccattt gcctggctct gggatttttt ctaagcattg taatttctgt aatgttttgc
      661 cttgtcaaaa aaatggtaga tgaataccaa caaaactctg ggcagtaata aacagcattc
      721 tgatagagag tgcctgtata aagctgaagt gacaatgacc gggaagggaa cccgttttca
      781 ggaatggatg cctgtctttt tcactgtaaa tatcctggca ttactaatgc taggatattt
      841 acagaagagc agcataacca tgacggaacg gggttaagtc aggttgtcct taacgctatt
      901 ttcaacctcg tatgccttct tcaggtttat gttcagatct catatctctc tcagcaatcc
      961 tcaataatca gatacaccgc tttcaccgga ccatgaaccc cgacgacttt gataagctca
     1021 atatccgccg ttgagctggg accactgatg atgttaatac atgacggcat tcgttcaccg
     1081 gcctgcgctt tctgatgcaa tttttctgcg agttgcgcta cacgcggcag gattgtgctt
     1141 ttacgcagga taaaaagaga agattccggg agcaggctca atgaacgccc gcgctcggcg
     1201 gcggaaaaaa gaaccacgcc tcccgattcg gttaaaccat attcagcata cacgacaccc
     1261 actttagcct gctctgcctg cgagatattc tcggcacctt tcgccggatc ccaaacaacg
     1321 gcattgcatt cctgctgcaa acgttcacta atccccaatt cctccagcct cgtgtcaccg
     1381 ctaatcacga ccgactgatc tcccagctct ttacacagac gtattgcagc ttctgccgcc
     1441 ttcgcctcgc tggtcagctc acagcgcgtc aacataacat cgctggcaaa ctgaataaac
     1501 gcgtcacagc gctgctgttg gttaagttgg gtaagccgct cgttagcata gttgttaagc
     1561 ggcgcatctt ctgcttgcgg ttcaagtcgc agcgggcgac ccagtgcctg agcaacgtta
     1621 ttcaaaaatt cgctccgatt atccattctt tttctcctgc gcctgatgtt tcttaaacca
     1681 actacggaaa ctctctccgt cagcttcagg aagatcgcgt gcttccatcc agtcgctaat
     1741 cgcgccaaat ttgagtggtg ttttgccgcc attgataaac cagcttgccg catgagcgcc
     1801 ggccatcatc ccgactttcc acaatcctgg atgactattg gcataagcga acatttttat
     1861 cgcccgttgc tctgcttttg cggtgatccc tttttcagcc atcacccgac gatgacgcaa
     1921 aatcagtttt gacagcggaa tacgcaccgg acacacgctg tcacaagcag tgcataaaga
     1981 gcaggcgtag ggtaaatctt taaaatcttt atagccgcca agtagcggag aaatcaccgc
     2041 accaattggc cctggataaa tagagccata tccatgaccg ccaatatggc gatatgccgg
     2101 acaagtattc atacaagccc cgcagcgaat acagcgcagc acatcccgaa attcagaggc
     2161 cagcacctca gaacgcccgt tatcgacaat aaccagatga aactcttcag gaccatcaac
     2221 gtgccccgct tcgcgcggtc ctgtcagcca ggtgttgtat cccgtcaaac gtgcaccaac
     2281 ggcactgcgc gccagcatgg tgatcaatac atctacctcg gcaaacgtgg gggcaatacg
     2341 ctccattccc atcactgcaa tatgcgtttt aggcagcgtg gtacacattc gcgcattacc
     2401 ttcattggtc accaggcata ccgaacctgt ctctgccacc gcgaaattac agccggtaat
     2461 acctatttca gcactgagga aatcttcgcg gattttttgc cggatgaata aggtcatcgc
     2521 ttcaggcgtt tccggcccct catagcccag acgttcgtgt agcactcgac ggatctgatg
     2581 gcgatcttta tgaattgccg ggaccacaac atgagatggc ggatcttgat ccagctggag
     2641 aatatattca cccagatcgg tttcaatcac ctgaatgcca gcatcctgca acacatgatt
     2701 gacaccaatc tcttcggtca ccatcgattt agatttcacc accttccggg catttttgcg
     2761 ttgggcaacc tgtaaaatgt agcgggtagc gtcttctttg gtttttgcaa accagacgtg
     2821 accgccgttt tgcgtcactt tttctgagag ctggtacaga taagcgtcga gattactcag
     2881 aacatgatca cgtatctggg cggcccgatc gcgccactcc tcccagtgcc ccaattcatc
     2941 gaccattttt tgccgatttg ccccaatacg ctgctgcgcg tttgccaccg ctttgcgcat
     3001 gatcggatct tcaatttgct gacggatgcg tgtcttaaaa tctgtattac tggttttgat
     3061 cgacatcttt atatcctcag cggctcatca acacttcagc aatatgcatc actttgactt
     3121 tctgcccttc ccgttgtaat cgcccactga tgttcagcag gcaactcacg tcagcaccaa
     3181 ttaaatactc agggcggact tccatcaggt gcacaacctt ttctttcacc atctcgccgg
     3241 atatttcggc cattttgacc gagaacgtgc cgccaaatcc gcagcaggta tcctgttcag
     3301 caaaggtcaa cagctccagt ccacgcacat ttttcagcag cgtaagtggc tcgtccttca
     3361 ctcccagctt acgggccagg ctacaagatg ggtgatacac agctctccct tgcaaactgg
     3421 cacctacatc gactacccct aatttattaa caataaaaga ggtgagatcc tgcatacgcg
     3481 cggcaacctt ttcggcacgt gatgcccatt caggttcatc cgccagatac gtcgggtaac
     3541 tttttacggc ataggtgcaa gagccagccg gtgaaataat gggatcgtcg ttatcctcca
     3601 gtgcggcgat cagatttttc atccctggaa tcgcttcttt gatataaccg ctattgatcg
     3661 caggctgacc gcagcatccc tgtttctccg ggaaatttac gcgacagccg agtttttcca
     3721 gtagcagcac ggagtctcgt gccattcttg atttcagggc gtcaccaata caggtgacaa
     3781 agaaattgac attcacaact aatactccat tacttcatgc ccatttatgc ccgatggttc
     3841 ccctccttcc gggggatcaa tcatcaataa atatggcgtt cttttttaat tggctagcaa
     3901 cataccaccc ctcatttgtg tgacaaatga taatctttca tccaaacttc aaatgcaata
     3961 actaatttta tttctttcgg catcgctcac attttatggg gaaatcgacg ttcatttttg
     4021 gcataagaaa tccatttaaa aaggctacat cccttatacc tattcagagc tacttattca
     4081 ggggttattg agatatacac tgatggtgta caaaaaaatc accgacaata atcacgtatc
     4141 aagtaatagt cggggtttta tttaattgaa gcaatttagt ttgcattttt atttaaaaac
     4201 tgagctagag tccatcagaa aaaaatatat aaagcccaaa ccaccgttgg aaatagaaat
     4261 agttgaaagg ctcacaactc ccggaatgcg gattatcttt gccctgattt ctggcggttt
     4321 tattcagggt gcaagctgtc tgaccctttc ccgatattct cccggagtac aaccaaactc
     4381 gcggacaaac gccttgtgaa aagatgattc actggcatag cctactgact cagcgatcac
     4441 cacaacaggg agcatttccc gggaaaacat ctgggccgct atttgtagac gcaactttgt
     4501 taatacagcc agcggcgtgg ttccggaaac atcacggaaa agctgggcaa aacttgcccg
     4561 agacatgtgg gcgatgctgg ccagcgattc gacggtccag gcgtgtcctg gcatttccag
     4621 catttgctgt attaccgcac caagacgtgg atgcagaagc aaactgagaa tgtttttctc
     4681 tgtattaacc tgtgcaatcc actcacgcac cgcgagggta aagaatgtag cgcagatctg
     4741 gctacacagt gcatccactc ccggcatcgc cgatctggat tcctgttgta aaaacgggat
     4801 cgcctccctc agccagttat attccacgct gtggttaacc ggtgctaaaa acagcgtttc
     4861 cggcaaagac gttaaaaaat aacgcgccga atgttgcaac cgaagagtgc cgcagacaat
     4921 acaggtcgat tcattatcga catgactcag gcgatgagct gaattttgtg gtagcaggac
     4981 cacatttccc gggcgtaatg taaaaatctc tcccgtcggc atttccagct tcgccgctcc
     5041 ttgcgttaac gcatgccaac gaataaccga taattccccg gcaccatgtt gaagctgcca
     5101 gtcacttcct aacacgcaat tcttatcgat cgttccttgt ggagcgttaa gcatcaacaa
     5161 acggctaagg gcatccatat cagactcctg agtggttttt gattacaaac accaacaatc
     5221 ttagtaaata actaatctaa taccatcaaa tggttaatca tgatttttgt tttgtgattc
     5281 tgattaagaa aaagaaaata ccagacgata agacaaaata tcaagcgttg tgaagaaatg
     5341 gtatttgctc ttttgcgtct atagtcatga tgtcaaatga acgtgtttcg acaggaaatc
     5401 atcatgaata aatatcaggc agttattatt ggttttggca aggctggaaa aacattagcc
     5461 gtcacgctgg caaaagcagg ttggcgagtg gctctcatcg aacaatcaaa tgcaatgtat
     5521 ggcgggacct gtattaatat cggctgcatc ccaaccaaaa cattggttca tgacgcacag
     5581 cagcacacag attttgtccg tgccatacag cgtaaaaatg aagtggttaa ttttttacgt
     5641 aataagaatt ttcataatct tgcggatatg cccaatatcg acgtgatcga cggccaggcg
     5701 gagtttatca ataatcatag cctgcgtgtt catcggcctg agggaaatct ggaaattcat
     5761 ggcgagaaaa tttttattaa taccggtgca caaaccgtgg ttccgccaat tcctggaatt
     5821 accaccacgc ctggtgtata tgacagcacc ggattactta atctaaaaga attgcctggg
     5881 catttaggta ttttgggcgg cggatatatt ggcgttgagt tcgcctctat gttcgctaat
     5941 tttggcagca aggtaaccat tttagaagca gcttcgctgt ttttgcctcg ggaagatcgg
     6001 gatattgctg ataatatcgc gacgatttta cgcgatcagg gcgtcgatat tatcctcaat
     6061 gcccatgtgg agcgaatcag tcaccatgaa aatcaagtgc aagtgcatag cgagcacgcc
     6121 caactggcgg tggatgcact gttaatagct tccggtcgtc aaccggctac cgcttcgtta
     6181 catccagaaa atgccggtat cgcagtaaac gagcgcgggg caattgtcgt tgacaagcaa
     6241 ttacatacca ccgcagacaa tatttgggcg atgggagatg ttaccggcgg gctgcaattt
     6301 acttatattt cactggatga ttaccgcatt gtgcgtgatg agttgctggg agaaggcaga
     6361 cgtagtactg atgatcggaa aaatgtgcct tattccgtat tcatgacacc gccactgtcc
     6421 agggttggta tgacagaaga acaggccaga gagagtggtg ctgatattca ggtggtgaca
     6481 ttgcctgtag ctgcaattcc gcgtgccaga gtgatgaatg atactcgtgg ggtattaaaa
     6541 gcgattgttg ataataaaac ccaacgtata ttaggggcat cactgctgtg tgttgactcc
     6601 cacgagatga tcaatatagt gaaaatggtg atggatgccg ggctgcctta tagcatatta
     6661 cgcgatcaga tatttactca tccgtcgatg agcgaatcac tcaatgatct attttcatta
     6721 gtcaaataaa ctcaaaatca gacgccagaa caaatattct ggcgtctcag agaaaagaat
     6781 cttattaatt ctctgttact ctataacttc ttaatcactt cattgatggt attttatatg
     6841 tttaaaaaat ctgttttatt tgcaacacta ttatctggcg ttatggcatt ttccaccaat
     6901 gcagatgata aaataattct gaaacatatc agcgtctcgt cagtatcagc atcaccgaca
     6961 gttctggagg ataccattgc tgatatagcc agaaaatata atgcttcatc ctggaaagtc
     7021 acatcgatgc gaattgataa taattcaacc gcaacagcag tattgtataa ataaggatgt
     7081 tcacaatgga aaaatacctg cacctgttaa gtcggggaga taaaattggc ctgtcattga
     7141 ttcgtctgag tattgcgatt gtttttatgt ggattgggtt attaaagttt gtcccttacg
     7201 aggcagacag cattacacca ttcgtcgcaa acagtccact aatgtcgttc ttttatgaac
     7261 acccggaaga ctataaacag tatctgactc acgaaggcga atacaaacca gaagcaaggg
     7321 catggcaatc ggccaataat acctatggtt tttccaacgg tcttggcgtc gtggaggtga
     7381 ttattgctct gctggttttg gctaatcctg tcaatcgctg gttaggttta ttgggaggac
     7441 tgatggcata taccacgccg ctggtaacac tctcattttt aatcaccacc ccggaggcat
     7501 gggtacccgc attgggtgac gctcatcatg gtttccctta tttatccggt gctggtcgct
     7561 tggtattgaa agatactctg atgctggcag gtgctgtcat gataatggca gattcggcgc
     7621 gggaaattct taaacaacgc agtaatgaat ccagttcaac gttaaaaact gaatattgat
     7681 aatccgaaca ctgtctgttg ttcacttttt caggcgtcag gccccctttc tgatgatgaa
     7741 gcctgacgcc atattaccgg agatatgaca gagtgaagat caatatcact cccctgttaa
     7801 aagattcttg tcgtctcagg taaaatatat gattaatatc aatcatgtat ttttacagca
     7861 agacaaccac gaacaaattc aggattaaaa tgttttaccg actccccaac ataacccaaa
     7921 tcaagcgaac taatttgtgc catttctttc tctgtcagtg agaaatccca gatagcaaaa
     7981 ttttcttcga tacgctgttg atgtgtcgat ttcggaataa cgaccactcc ccgttgaata
     8041 ttccaacgca gaatgacttg agaaatcgat ttttgatggg catctgcaat actctgaagc
     8101 agattatttt caaagggttt atgtcgtcca ccacctaacg gagcccatgc ttcaggctgc
     8161 acgttataat acttcatggt tgccagcgcc tctggttggg caaaataggg atgcaactcg
     8221 acctggttga ccatcggttt aactcttacc gtttcacaaa agttcgccag aacatgagga
     8281 tagaaattgg aaaccccaat tgcttttaat ttgccctctt cataggcatc ttcgagtgca
     8341 cgccacacac tgaaataatc gcgcatagcc tggtgcaata aatagaggtc gaagtactct
     8401 agtcccgatt tttttaatga tgcttcaata cctgctgctg ccgtatcctg attcaacata
     8461 tcctgccccc acaatttaga cgttataaat aactcatctc gggtacataa accttcagat
     8521 atagcttcac gaacggcttc accgacggca tcttcattac cgtataccgc tgcagtgtcg
     8581 atgagtcgat aacccgtgcg gatagcgttc aacactgact gtttgcacac gtttttatcg
     8641 gtaacctgaa aaacaccaaa tcccatcatc ggcattttca gattgttact taatacggaa
     8701 aattccactg tcagtccctc cattaggcat gaacaatgag tctacgttaa aacataacct
     8761 caaattagta tgtggatttt gatatcactt atgcaaaaaa ttcattaata atgtaggact
     8821 gaaacctctc tattttcggg gacaacgaag cagacgctac cagtgctttt gccttcgacc
     8881 ttgctgtttt tgatacactt agggccctgg gcataacgaa aatgtgcgat atcacgaaga
     8941 attaatggag aat
//
LOCUS       DAKISA010000148         8832 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.149, whole genome shotgun sequence.
ACCESSION   DAKISA010000148 DAKISA010000000
VERSION     DAKISA010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8832
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.149"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..615)
                     /locus_tag="OVW45_003889"
     CDS             complement(<1..615)
                     /locus_tag="OVW45_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCV5528225.1"
                     /translation="MADSQFYRPGLPQLISMIRSDLLTRFEQDTLLRRMDAEVYARVQ
                     AAAVHTLYGYIDYLARNLLPDVCDEDWLYRHARIKRCPRKEAVAARGFVRWDGVEGTP
                     VLPAGTQIQRDNQVTFTTTATVTAADGLLRVPVVADEPGSAGNTDDGIAMQLGTPVSG
                     LPSTGYADTITDGEDIENLEIWRARVMERYYYIPQGGAGNDSNLL"
     gene            complement(608..1018)
                     /locus_tag="OVW45_003890"
     CDS             complement(608..1018)
                     /locus_tag="OVW45_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HCV5528226.1"
                     /translation="MMINVNGRPVSTGASIDLLTRAVIISLFTWRRAGRDDAPRIFGW
                     WGDTWPAVQNDRTGSRLYLLRRSKLTNKTPQLARDYVREALAWMVEDGVASRLDINAV
                     RTGTDSLALAITIYQRDGNIHNIIFDDIWSELNG"
     gene            complement(1024..1557)
                     /locus_tag="OVW45_003891"
     CDS             complement(1024..1557)
                     /locus_tag="OVW45_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HCV5528227.1"
                     /translation="MNGFSLRNLISRAVITAVDSARKCQSVGLKMIAGDQKQHVEHLE
                     PYGFTSAAQNGAEGVALFPAGDRSHGVVVVVADRRYRLKGLKRGEVALYDDLGQSVVL
                     TRSGIVVDGAGKAIIFKNAPKARFEMPVESTADITDNCDSGGLSMQQMRQAYNAHKHT
                     ENGDGGGITDTPDQPMG"
     gene            complement(1535..2611)
                     /locus_tag="OVW45_003892"
     CDS             complement(1535..2611)
                     /locus_tag="OVW45_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HCV5528228.1"
                     /translation="MNDTVLLRVSGREWGGWTSVRISAGINRIARDFNVAITTRWPGS
                     RDYQPRIKNGELVEVLIGDEPVLTGYVEALPLRYDASSVSMGIVGRSKTADLVDCSAL
                     PLQQSGKNLLRIVSELAAPFGITVVDAGVPQTAVIDAQPEHGETVADCLNRLLGQVQT
                     LAYDDECGRLVLGKPGTGKAATALVLGENILSCDTERSIRERFSEYQVSGQRPGNDDD
                     FGEATIAAIRQTIQDSGVTRYRPLLIQQSGTATTATCKARCEFEARQRAALTRETTYT
                     VQGWRQGSGALWRPGLSVIVFDPLNNFDNDELVIAEVTYNQDDRGTTTELRVGPADAY
                     LPEPVTARKKKMLRRISDERVFSS"
     gene            complement(2608..3999)
                     /locus_tag="OVW45_003893"
     CDS             complement(2608..3999)
                     /locus_tag="OVW45_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HCV5528229.1"
                     /translation="MDLPSLPSLSSLFSASSGINWRDNLYDASFRGVPFSVESDEGSF
                     GRRVQVHEYPNRDKPYTEDLGRATRRLTINAYLVGDDYAEQRDRLITAIETAGPGTLI
                     HPQFGEMQGCIDGQVTVSHSGTEGRMCRVSFQFVESGELSFPVAGAATARKLEESSVF
                     LDELIEDMFGDFDLAGIPDFIQNDVIARTTDMLGTVQTAFKMVNSAVSAGARLLQGDL
                     SVILMPPSVASDFVHTLQDTWRAGTRLVDNTQDLVQSITTMSGITLDPGLAPRAVWPT
                     DSASVIRQKQQTNLVAAVIRTTAISEATRAVTSLPQPGSLVKNQQAVVAVGGSTERQS
                     DIIHVSHPALDSVAASTGQDERAQPPTRENLTIIRESLNAAIEQELRRTADDRLFFQL
                     TSLRTELNRDIQARLVQTEETAERTPAEVLPALVLAASWYDDASRETDILDRNAISHP
                     GFVPVRALRVPVR"
     gene            complement(4046..4528)
                     /locus_tag="OVW45_003894"
     CDS             complement(4046..4528)
                     /locus_tag="OVW45_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528230.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNVKTSADNDVD
                     LDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIGVFDFANNASPATMTFLIPIYKD
                     DDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAGVNVNGESGVTYENCKLNMKDINDD
                     "
     gene            complement(4596..6545)
                     /locus_tag="OVW45_003895"
     CDS             complement(4596..6545)
                     /locus_tag="OVW45_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCV5528231.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLLLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKAHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(6630..6965)
                     /locus_tag="OVW45_003896"
     CDS             complement(6630..6965)
                     /locus_tag="OVW45_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCV5528232.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPRSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(6965..7321)
                     /locus_tag="OVW45_003897"
     CDS             complement(6965..7321)
                     /locus_tag="OVW45_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCV5528233.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(7321..8817)
                     /locus_tag="OVW45_003898"
     CDS             complement(7321..8817)
                     /locus_tag="OVW45_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCV5528234.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
BASE COUNT         2080 a         2598 c         2015 g         2139 t
ORIGIN      
        1 caataagttg gagtcattac ctgcgccccc ctgtggaatg tagtaataac gttccataac
       61 gcgggcacgc catatttcca gattttcaat atcttcaccg tctgtaatgg tgtcagcgta
      121 ccctgtggac ggcagaccac tgacgggtgt tcccagttgc atggcaatac catcatccgt
      181 attccccgcc gatcccggtt cgtctgccac aacaggcacc cggagaagac catcggctgc
      241 ggtcaccgtc gccgtcgtgg taaaggtcac ctgattatca cgctggatct gcgttcccgc
      301 tggcaatacc ggcgtcccct ctacgccatc ccagcgcaca aatccccggg ctgccaccgc
      361 ttcttttcgc gggcagcgtt tgattctggc gtgccggtac agccagtctt catcacacac
      421 gtccggtaac agatttctgg caagataatc gatatacccg tacaacgtgt gtacggctgc
      481 agcctgtaca cgggcataca cttccgcatc catacgacga agcagcgtat cctgctcaaa
      541 gcgggttaat aaatcgctcc ggatcataga aataagttgc gggaggccgg ggcgataaaa
      601 ctgactgtca gccattcagt tcactccaga tatcatcaaa aataatgttg tgaatattgc
      661 cgtcacgctg gtaaatggta atggcaagtg ccagcgagtc tgtcccggtc cggacagcgt
      721 taatatcaag acgggaagca acaccatcct ccaccatcca cgccagcgcc tcacggacat
      781 aatctctggc aagctgcggg gttttatttg tcagcttgct gcgtcgcaac agatacaaac
      841 gcgaccccgt gcgatcattc tgaaccgcag gccaggtatc cccccaccat ccaaatatcc
      901 gtggtgcatc atcacgcccg gcacgccgcc atgtaaaaag cgaaataatc acagcacgcg
      961 tcagaaggtc gatcgaagcc ccggtcgaca cgggtcgccc gttaacatta atcatcatga
     1021 ttttcagccc atcggttgat ccggcgtgtc agtgatcccg ccaccatcac cattttcggt
     1081 atgtttgtgg gcattgtagg cctgccgcat ttgctgcatg ctgagtccgc cactgtcgca
     1141 attgtcagta atatcggcgg tggattcgac tggcatttca aagcgcgctt taggcgcgtt
     1201 tttaaaaatt atggccttcc cggccccgtc caccacaata ccggaacggg tcaggacaac
     1261 ggactgtccc agatcgtcat aaagcgccac ttccccacgt ttcagtcctt tcagccggta
     1321 acgtctgtca gccacgacca caaccacacc atgagaacga tcgcccgccg gaaataaagc
     1381 aacgccctca gcaccgttct gtgcggcaga tgtaaaacca taaggttcaa ggtgctcaac
     1441 gtgttgtttc tgatctccgg ctatcatttt caaccctaca gactgacact ttctggcgga
     1501 atccaccgcc gtgatgacag cccgggaaat cagattacga agagaaaacc cgttcatcag
     1561 aaatcctcct caacattttt ttcttcctgg cggtaacagg ctcggggaga taagcatctg
     1621 ccgggccaac ccgtaattca gtcgtcgtgc cccggtcgtc ctggttatag gtaacttctg
     1681 cgatcaccag ttcatcatta tcaaaattat tcagcgggtc gaaaacgatg acagataacc
     1741 ccggacgcca taacgcgcca ctgccctgcc gccagccctg aaccgtatat gttgtctcac
     1801 gggtaagcgc agcccgttgg cgcgcttcaa attcacaacg ggccttacag gttgctgtcg
     1861 ttgctgtgcc tgactgctga atcaacaaag ggcgataacg ggtcacgcca ctgtcctgaa
     1921 tggtctgacg tattgcggca atggtggcct caccaaaatc atcgtcgttg cccgggcgct
     1981 gcccactgac ctgatattca gaaaaccgct ctctgatgct tctttccgtg tcacaggaaa
     2041 gaatattctc tcccagcacc agcgccgtcg ccgctttacc tgttccgggt tttcccagaa
     2101 ccagtcgccc gcattcatcg tcataagcca gcgtctgaac ctgccccagt aaccggttaa
     2161 gacaatcggc aacggtttcg ccgtgttccg gctgggcatc aatcaccgct gtctgcggca
     2221 cgccagcatc aacaacggtg atgccaaatg gcgcagccag ttcactgact attctgagca
     2281 ggttttttcc gctctgctgg agtggcaaag cagagcagtc aaccagatcg gctgttttgc
     2341 ttcgcccgac aattcccatg ctgacgctgc tggcgtcata acgaagcggt agtgcctcca
     2401 catatccggt gagcacaggc tcatccccga taagcacttc aaccagctca ccatttttta
     2461 tccggggttg ataatcccgg cttccgggcc agcgggtggt aatggcaaca ttaaaatccc
     2521 gggcaatacg gttaatgccc gcactgatac ggacggatgt ccagccgccc cattcgcgac
     2581 cggaaacccg aagtaaaacg gtatcattca tctgacgggt acccttaatg ccctgaccgg
     2641 aacaaagccc ggatgagaga tggcatttcg atccaggata tcagtttcac gggacgcatc
     2701 gtcgtaccat gacgcagcca gaacaagcgc aggcagaact tccgctggcg ttcgctctgc
     2761 ggtttcctcc gtctgaacca gacgcgcctg aatatcccgg ttcagttctg tacgtaatga
     2821 cgtcagctga aaaaacagcc tgtcatccgc cgtacgcctg agctcctgtt caatcgccgc
     2881 gttcagcgat tcgcgtatga tggtgagatt ttcccgcgtg ggtggttgcg ctctctcatc
     2941 ctgtcctgtg ctggctgcca cgctgtcaag tgccggatga gaaacgtgaa taatatcgga
     3001 ctgacgttca gtggaaccac caacagccac aaccgcctgc tgatttttca ccaggcttcc
     3061 gggttgtggc agtgaagtga ccgcccttgt ggcttcgctg attgccgtcg tccggatgac
     3121 agcagccaca aggtttgtct gctgtttttg cctgataacc gatgcggaat ctgtgggcca
     3181 caccgcacgc ggtgccagtc ctgggtccag cgtaatgccg gacattgtcg ttatggactg
     3241 caccagatcc tgtgtgttat ccaccagccg ggttccggcc cgccaggtat cctgcaacgt
     3301 atgcacaaaa tcactggcaa ccgacggcgg catcagaatg acggataaat cgccctgcag
     3361 tagtctcgct ccggcagaaa cagccgaatt aaccattttg aaagctgtct gaacggttcc
     3421 cagcatatcg gtcgtccggg caataacatc gttctgaata aagtccggta ttcccgcgag
     3481 atcaaaatcg ccgaacatgt cttcaatcaa ctcatccagg aatacggacg actcctccag
     3541 ttttctggcg gttgcagctc cggcgaccgg aaatgacagt tccccgctct caacaaactg
     3601 aaatgaaacc cggcacatac ggccttcagt gccggagtga gaaacggtca cctgcccgtc
     3661 aatacagccc tgcatttcac cgaactgcgg atggatcagc gtccccggcc cggcggtttc
     3721 aatcgctgta atcagcctgt cgcgctgctc tgcgtaatca tcaccaacga gatacgcatt
     3781 aatcgtcagc cgtcgcgtgg cacgcccgag atcttccgtg tacggtttgt cacggtttgg
     3841 gtattcatgg acctgaacgc ggcgtccgaa cgaaccctcg tcgctctcca ccgaaaacgg
     3901 aacgccacga aatgaagcgt cgtaaagatt atcgcgccaa ttgatacctg aagatgcaga
     3961 aaagagtgaa gaaagagaag gtaaagaagg taagtccata ctgtctccac tataaaaaaa
     4021 ccgccaattt ctggcggctt atatattaat catcattaat atctttcata ttcaatttgc
     4081 aattttcgta ggtcactccc gactcgccat taacattaac tccagctctg atattcccgt
     4141 tttcttttac atctacaaaa aactggcctc ttggcatctt gaacgcaaat gtaaaaccaa
     4201 cagtatctga atcaaaatca tcatctttat atattgggat caaaaatgtc atcgtcgctg
     4261 gtgatgcatt gtttgcaaaa tcaaatacac ctatactttt gccgaattca taagaagctt
     4321 ttctcaatat aatcgcaccg ctggcagaag gggatgggca cgatatcctc agttgtctga
     4381 atccatctgt atccaagtca acgtcattat ctgcagatgt tttaacgtta ctaataaatc
     4441 gtgaaacaat ctcctcattt gttagtattt tcgcctgagc acagcaagag gttaatattg
     4501 caaccgtcat taaaaatggc tttatcacaa aacaaactcc attgttagca tgttacgcga
     4561 taattttgcc tcatctttca ccacaaaaaa agaactcacc ttttaaatgg agaataccca
     4621 acatcatggg tgattttcat aaagggatcg cctgttttcg gcaaatcgat aacgcgcatt
     4681 ccaggcggcg cgttgtcaaa cgtcacctta agttcgctgc gggttgccgg tgaagatgca
     4741 aggcttaaca gctgattcct ttccagtgat acattcgggg tgtagcgttt atcgggagat
     4801 aaatacccat caccacgtgg catctgccat ccggtcatgt catagacaaa atcatggaac
     4861 tgtttgcccc actcgtcgaa cttatcattc agaccaaatc cactgttcag tgcatcagcc
     4921 acaaaatttt taacaagcca gggatgtgct ttttcaaact cctgcgccat catgcccagt
     4981 tcaagcaatc caccaatcag actgatcttt ccgaggccct ttaatttcag ccctgttttt
     5041 cctgccgccg ttgtccattg attctgggcg actgttgccg cacgaatact tgccaccgcc
     5101 ttcacaccaa tatacagggc agaaaccgtt gctattgttt ttactgcctg ttcccatccc
     5161 cccattgctc tgacaacctg atcaacctcc tgccagactt ttttcacaac cggaccgacc
     5221 gtttcccagt tatcaattat gagatatgca ccaccgacga gaagcgcaat taatcccttt
     5281 gccggcgtca tattcatcac gctacccatg atttttgtta tgcgtgtcaa tgtgccaatc
     5341 gccacgccca tcgtcagtaa tgccgcacca gttttcgcga tggtttttac gacctcagga
     5401 ttttctctga caaacgtgcg cacttcttcc agaaaaggct tcatctcttt tatgccttca
     5461 ttgagtgaag gcaggaaggt ttcccccagc gtggaagaaa tcgcattgat ctggttctgc
     5521 agcagcaaca actgattttc tgtcgtcgcg gcacgggcgg catattcttt ctgcattgag
     5581 ccgccatact gctgcgcatc cgccacacga ttaaagttgg ttcgcaacaa atccagattc
     5641 gtgagcagcg gcgcaatggc ccccagggat tccttcccga acagcgcttt cagcacagca
     5701 gcctgttttt ctttcggcac ttttgccaga gaatccagta cgtgcagcat ggccccgcgg
     5761 gcgtctttct gcatatccgc cgccagtttt ttcgggctaa tgcgcaacga gcggagcact
     5821 tttttctgcg atttggtggc agaatccctc gctgtcagcg acaacataaa attctttatc
     5881 cccgtcgcag caatttctga ctccaccccc attccggcaa tggttgcccc cattgcggca
     5941 atctctccgg aggccacacc cgcaacactg cctaaagggc caatacgggt cacaacatca
     6001 gaaatctttt tcgcacttgc aggaccggta ttaccaagat agttaatctt atccgcaagc
     6061 cctgccacct ctccctgtgt cagtttaaag gcagtgcgcc actgcgccat catctgaccg
     6121 gattcttctg ccgtggtgtc aaaggccacg cccatcttca cggcatcgtc agtaaactgc
     6181 atcagctcgt cacgggcgat accagcctga ccacccgccg ccacaatttc ggcgatacct
     6241 tccgccgaca tgggcagttc tgttgacagg tcgcgcacct gctccgtcat tgccttaaac
     6301 gcttccggcg tatccagacc atccaccact ttccggacat ccgccatttt tgattcaagg
     6361 gcaatggcag atttgaccgg gagcgccagc gcccccagta ctgcggttcc ggcacccgct
     6421 gcgcccagta acaggctggc aaactccttt ttaaaacctt tcagctggcg ctgcattccc
     6481 ttcagcggtg ccgatacctt atccacggca gtgatgatcg ccttcagctg aaaactgtca
     6541 gccatgcttc atctcctcgt taatgcggac ggcctcggct tccagttctg taaactgcga
     6601 aacagccccc cgtcgcagct ccagtggatt cagtttccag aaccaggcaa cattgtagag
     6661 tcgtttccgg aggtgcttcc cgtctccgac tgggtaaaaa aacgcaggat ctgcatgctg
     6721 gtcttaaaaa tatccagttt tgccatctgc gctgccgatg aacgcgggat ccccgccagc
     6781 aacgggatat atttcagtgc cacctggctg tccagtttaa tactgccttc accggaaata
     6841 atgaaaggaa aacccagcgc ctcgatttcg tcatacgtgg gctcgcgcaa ctccagcaca
     6901 tgcagcattt cgttatgcgc tgtcaccggt ttttttaaaa ctatttctgt aacgttcatt
     6961 gccattactg ataccctccc tcttcgccgt ggaattcaag atctgccgtg ccttcttcgg
     7021 cattatgatt agcctctccg tgcagccatg ccgccgaaag cacatacacc atgccgtttg
     7081 ccagctcagc ggtaatcgtc atctggtctg aggtggtaat tttgtcgacc ggaaagtttt
     7141 tgggcacttt aaacgtgccc ttaacgtaag gtgaccgcca ggtctccttg taatccacat
     7201 cccctgccat gccgacgatg tcatcccgga cattggtgtt catcggcact tcaatgccac
     7261 ctgtcagcga taactgctga ccatccactt taaaaaaaca ggttccggcg atctttgcca
     7321 ttatgctgac tcctctgaat actgaagacg gaactggtta accaccgcaa agacacgtaa
     7381 ctgattaaca taatccggcg ggaacagcgt attcagtcgg ttcgggttat ctgcatcacg
     7441 ctcaactatc agatactgtt taaacagatc gtaattttcc acaataccgg cacgctccat
     7501 ctgacgatat gtcgccagaa gttccccttt gataacggca ggagtgacaa tcgcctgccc
     7561 cggaccaaaa cgggtaccat catttgccag cttgtgacgt ccgtacttgc ttgtgatgac
     7621 cgatttcagt ttgcgaagaa catatgcact ggtatgcaga gtttcactgt ccagatagct
     7681 gttgtctgcc acgccatacg cattcttttt gtaggtggtt acggaacgct ggatctgcaa
     7741 cgtgccgcct tccacataag ccgtcgccac gccgtgagat aaaagggtct gctgctctgt
     7801 catgatgaat cgcttacctt tcggtgccgg aagcatcccc accagctccc ccgtctgtgt
     7861 cggacgggca ggatcattcc ggataaatac cgcttcacgg gcaaggcgac tggcaaccag
     7921 ttcatcgaca ggcgactggg tttctttttc gtaaccggca agcgtgatat gttgctgatt
     7981 atgcatatct ccggcaccaa ccagctctga cagcgttccc agttttgcgg tatagacatg
     8041 cccgtataac tggcgcgcat aactccagcg accgctgctg tcattcattt cggtcatcat
     8101 catattgatg gaggcggcat cgttgaacgg cagaccgata aagtcgaatg cctcatcgcc
     8161 catagcagca acagcggcgg taagatcagg cgctccgcta ccggcagcac cgacttccgt
     8221 caccacccga agccctgctg gcagaatctc accaccacca gaaccataat aattcagaca
     8281 gaccggtaac tcattaccat acagcccctt atgacgggca gtcagcgtca ccacacctgc
     8341 atcagatgac gccgtaaacg gcagggtgat aaccccgttt accgcttcct taatcgcggt
     8401 ggcaaccgca gtggcatcat cgccattcac cacaggcacc tgtacacggg agcgcccgac
     8461 atacagactc agggtgccgc tttcctctgc ttctccggca accgtcaccc tgaccgtcgc
     8521 cgccgcccct ctggcttccg gtactgcaat aacatacagt tcaccgaaag gatctgtctg
     8581 acggtagact tccaccatac gcgccagctg gctccccgcc ccacaaatct ggcgggcata
     8641 atctgccgat ggcatcagca ccaggctgtt aacctcaatg gctgcatcgt tgctggcatg
     8701 cccgatcagt aatgcaggcg cgctggtcac cgctgtattt gccgcagaat tgttcatctc
     8761 ggcgtagaac aacggcacca gcgtattcga cggaacagta ttaaaactta tcgtcatgat
     8821 tttttagcct ta
//
LOCUS       DAKISA010000149         8632 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.150, whole genome shotgun sequence.
ACCESSION   DAKISA010000149 DAKISA010000000
VERSION     DAKISA010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8632
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.150"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(143..2686)
                     /gene="mdoH"
                     /locus_tag="OVW45_003899"
     CDS             complement(143..2686)
                     /gene="mdoH"
                     /locus_tag="OVW45_003899"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCV5528235.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGAEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2679..4214)
                     /gene="mdoG"
                     /locus_tag="OVW45_003900"
     CDS             complement(2679..4214)
                     /gene="mdoG"
                     /locus_tag="OVW45_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCV5528236.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4608..5765
                     /gene="mdoC"
                     /locus_tag="OVW45_003901"
     CDS             4608..5765
                     /gene="mdoC"
                     /locus_tag="OVW45_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCV5528237.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5773..7254)
                     /gene="clsC"
                     /locus_tag="OVW45_003902"
     CDS             complement(5773..7254)
                     /gene="clsC"
                     /locus_tag="OVW45_003902"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCV5528238.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLSDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7196..7729)
                     /gene="ymdB"
                     /locus_tag="OVW45_003903"
     CDS             complement(7196..7729)
                     /gene="ymdB"
                     /locus_tag="OVW45_003903"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCV5528239.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7824..8135)
                     /locus_tag="OVW45_003904"
     CDS             complement(7824..8135)
                     /locus_tag="OVW45_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCV5528240.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8256..8588)
                     /gene="csgC"
                     /locus_tag="OVW45_003905"
     CDS             complement(8256..8588)
                     /gene="csgC"
                     /locus_tag="OVW45_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCV5528241.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WPPSSEKS"
BASE COUNT         2155 a         2246 c         2052 g         2179 t
ORIGIN      
        1 ataacgccgg tatttttatt ggctcgttgt ttttgccgga tgcgacgtga acgctttacc
       61 taacccacaa aagcttgcaa aatcaataaa ttgcatgaac gatgtaggcc tgataagcgt
      121 agcgcatcag gcaactacat ttttattgcg aagccgcatc cggtttacgc aatgccagtg
      181 gattgagctt tatcccttcg taataactca cccatgaaga atatctctca ggggaattcc
      241 agacgcggaa atgcagacgg gccatcgtca ccggatcgct tagcagcaca aggcgacgat
      301 cgcgattcag cttctctggc gtctcgttca gcgcctgttc aacgtggcgg tcacgggcga
      361 tttccagcac cttactggcg cggtgacgcg cggtcgccat tgcggttgcc agagcgttaa
      421 atgacgggtt aaacactgcg tgcataaagc catcgtcaag ggagcgttgg cgattcatct
      481 caaggaaccg atcggtatca accagcacct gcggcggtga atactcttcc gggatcagga
      541 acagtttcca gcgtttggtg cgcagaccaa cggtggcacg gctggaaatc accgaaacaa
      601 acggtgacag gatcaacgag aagacaatcg gtgccagcca gaacaggaaa cgcagatcca
      661 gccacgccat cccaacagcc cacactaacc ctaacagcag ctgtgagccg tggcgtttga
      721 acgcttcacc ccaggaagtg gagtcatcat cacgctgcgg tgaattccac accacttccc
      781 agccaaggaa cgcgctgacg acgaagaccg tatggaacag catgcgtacc ggagccagca
      841 gcacggaaaa cagcacttcc agcagcaacg ataatgtaac gcgccagaag ccgccgtatt
      901 ctttcgttcc tttgcaccag ataagcaaaa tgctcaataa cttcggcagg aacaacagca
      961 ccatggtcga agcaaaaagt gcaatcgcca gctcaggacg ccactgcggc cacaccggga
     1021 acaactgccg tggttgcagg aagtattgcg gttcggtcaa cgcatgtact acctgcaatg
     1081 cagtagagag cgcgaggaac ataaaccaca gcggagcgga gagataagac atcacgcccg
     1141 tcaggaacac cgcacggtga accgggtgca tacccttcac caggaacaga cggaagttca
     1201 tcaggttacc gtggcaccat cggcggtcac gttttagctc atcaagcaag ttaggcggca
     1261 attcttcata agaacccggg agatcgtaag caatccagac cccccaacct gcacggcgca
     1321 tcaacgccgc ttccacgaag tcatgtgaca ggattgaacc ggcaaaggaa ccttcgcccg
     1381 gcagcggagc cagtgcgcag tgctcgataa acggtttcac gcggataatc gcgttatgtc
     1441 cccagtagtg cgactcgcca agttgccaga agtgcaaacc ggctgtaaac agtggcccat
     1501 acacgcgggt cgcgaactgc tgacagcgcg catacagcgt atccataccg gacgctttcg
     1561 gcgacgactg aatgatcccg gcgttcgggt tggcttccat caggcgcacc agcccgcaca
     1621 aacaatcacc ggtcattacc gagtcagcat ccagcaccac catgtagctg tactggctgc
     1681 cccagcgacg gcagaagtca tcgatattac cgcttttacg cttcacgcgg cgacggcggc
     1741 ggcgatagaa aatctgacct tcggcaccga cttcagcgat aagctccatc caggcttttt
     1801 gctctgcgac gcagatatcc gggttataac tgtcactaag aatgtagaca tcaaagtgtt
     1861 tggcattccc ggtggctttt actgattccc acgttgcacg caggccagca aaaacacggt
     1921 tcacgtcttc gttacagata ggcatgatca acgccgtgcg atgctccggg tttaatggtt
     1981 catcgccaac tgttgacgca gatatactgt atttatcgcg accaataagc agttgcagga
     2041 agcccattaa cgccgtccag aatccggcgg acacccaaca gaacagtacc gcaaagagga
     2101 tcaggatacc ggtttgcagc atataaggca gaagctgcat aaaggaaacc cacaaatcct
     2161 gaccaaccat atccatagga ttaatcagcg cccaaccctg ataaggaaga atggtcttca
     2221 tataccaggt cgcgacgaca gtttgcgcga gcgtcaggat caacagaatg taacggcgga
     2281 tggtaccgac ggtacgccac ttttgctcgc tctcctgctc ttctttggtc aaacgagcca
     2341 gatagcgcgg cgtgacatcg cgtccacgca ggcgatccca gaaacggcct accgggttgg
     2401 tacgccacgg gtcgggaaac atcgaggagc gttttgcttc tggcatcgcc ttcagctgat
     2461 cgcgcccttc gtcgtcttta attaactgtc catcagcaag tgaatctggc caggcttgtt
     2521 ccagacgcgc ctttaccgag ccttgcgggg aatcatcctc ccgcgcccag gtgcggtgtt
     2581 cggcatccag cgcctgatga acggcgcgga tatcagtctt cggcaatgcc gctttctcgc
     2641 ttgcggcgat gggcattgcg tcaatgtact cagttgtctt attcattggc aggtaactgg
     2701 tagctccagg tttcactcaa cgtctgatcg gcattcacca gcgcagcacg catttcagtg
     2761 gttttcttgg catctttcac tttcacacgc atcaccagac gccagccttt ggtaaccggg
     2821 ttatagcgca ccgtgctttc aactatctca ccattatcac caatgctggt ttgcgctgtg
     2881 accggggtat cctctggcag ttttttcatc tctgcgccgg taaaatcgac cacaaaggcg
     2941 atagtaccgt caggctggcg aatcaggttc gactgcttca catcccccgt tgaacgacgc
     3001 gtttgttgca cccatgcgtt atctggcgca tgcagtttgt cttcatcacg gctgaaggtg
     3061 atggtgtatt taaagttcat ctctttaccc ggctccggca gctgatccgg cgtccagtaa
     3121 gcgacgatgt tatcgttggt ttcatcgttg gttggaattt ccaccagctc aacgctgcct
     3181 ttaccccact cccctttcgg agtcacccat gcgcttggac gaagatcgta acgatcatcg
     3241 agatcttcaa agcgggagaa atcacgaccg cgctgcaaca gaccgaagcc ttgcgggttt
     3301 tccatcgaga agctgctaac cgctaaatgt ttcgggttat tcaacggacg ccagatccat
     3361 tcgccattac cggcatggat agagagaccg ttagagtcgt gcaactccgg acgatagtta
     3421 tttgcaggcg acggttggtt cggcccaaac aggaacatac tggttaacgg tgcaaccccc
     3481 agtttgccga ctttatcgcg cagatagatt ttcgactgca catccacaac cgtgtcacgc
     3541 cctggcataa ccacgaattt gtaagcacct gtcgcgcgcg gcgagtcaag caatgcataa
     3601 atggttaaac gtttatcagt cggttttgga cgctcgatcc agaactcttt gaagcgtgga
     3661 aattcttccc ccgatggcaa ggcggtatca attgccaggc cgcgggcaga aaggccataa
     3721 acctgacctg caccaatcac gcggaaatag ctggccccga gcatgctgac gatttcatcg
     3781 tttttatctt tgctgttgat cgggtaaagc actttaaaac cggcaaaacc aaggtctttt
     3841 accgtgtctt tgtcatgctg aacatcgccg aaagtgaaat aatccgggct gtatttgatt
     3901 cgtttgactg cggtggcagt cacttcattt attttgaccg gggtatcgaa gtacataccc
     3961 tgatggtaga actcgagttt gaatggggtc ttcagattgt tccagtacgc tttgtcatga
     4021 ttaaactgga tctgctgata gtccgcgtat ttcatatcgc ggaaaacgga gggcaagttg
     4081 cttttgggcg cctcatagcc tttcccggct aaggattgag cttgctttgc gacatcatca
     4141 atactgaaag cccagcttga agatgtatac agggttaaca ttactgcagc actcaaccaa
     4201 cgcattttca tcatttgtag tttatgtttc ataattagta agcacttccc cctttgtgtg
     4261 cttatatcga tccgatctat tttaatggat aaccagccat tccgacaaca gaatcccggt
     4321 atcgttcagc acgctggctc atggattaag cacctgattg caaccgtttt cactttccgt
     4381 gcttctccta aagacagaag catcgacttc gtgtagggtt tgccagcaat tttatttatc
     4441 aaccgtctta cagacgcttc cattctggtt agtggtagcg atgatgctat gcataaaaac
     4501 cttgaagcct gcgcctggcg cggtcaggtc ggtgtcgcta tggcaacaag cctaaaggca
     4561 taacccgaca gggataagac gaaaaatccg agattagtta accatatatg aatccagtac
     4621 ccgcgcaacg tgaatatttc ctcgactcca tccgcgcctg gctgatgttg ttagggatcc
     4681 cttttcatat ttctttaatc tattcgagcc atacatggca tgtgaatagc gccgaaccgt
     4741 cattgtggct gacccttttt aatgacttca tccactcgtt ccgcatgcag gtatttttcg
     4801 ttatatccgg ctacttttcc tacatgcttt ttttacgcta tcccttgaaa aaatggtgga
     4861 aagtacgtgt cgaacgtgta ggtatcccga tgttaacagc catcccctta ctgacattgc
     4921 cgcaatttat tatgctgcaa tacgtcaaag ggaaagcgga aagttggcct gggctgtcat
     4981 tgtatgacaa atataatacg ttggcctggg aattaatatc acacctgtgg tttttactgg
     5041 tgttagtagt catgacgacg ctgtgcgtat ggatatttaa acgcatcaga aataatttag
     5101 aaaattctga taaaacgaat aaaaaattct cgatggtaaa actatcggtg atttttttat
     5161 gcctcggcat cggctatgcg gtaataagaa gaacgatttt tattgtgtat ccgcccattc
     5221 tgagtaatgg catgttcaat tttattgtca tgcaaacgct attttatttg ccgttcttta
     5281 tcctcggtgc actggctttc attttccctc atcttaaagc cttgtttacc acgccgtctc
     5341 gtggctgtac ccttgcagca gcattggcat ttgtcgctta tttactcaac cagcgctatg
     5401 gcagtggcga tgcctggatg tacgaaaccg agtcggtgat caccatggtc ctcggtctgt
     5461 ggatggtgaa tgtggtcttc tcctttggcc accgtttgct taacttccag tcagcgcggg
     5521 tgacttactt tgttaacgca tcgctattta tctatctggt tcaccacccg ttaacgctgt
     5581 ttttcggcgc atacattaca ccgcacatca cctccaactg gcttggtttt ctctgtggcc
     5641 tgatattcgt agtagggatt gcgataattc tgtatgaaat tcatttacgc atcccgttac
     5701 tgaagttttt gttttctggt aaaccggttg ttaagcgtga gaacgataaa gcaccagccc
     5761 gttaagccac atttacaata accattccac gggcaatatc gacgccagtc tgaccataac
     5821 ccgcttccag aaactggtgg cgggttcttt tttgagaata atctctttct ttgcatgacg
     5881 atcaacccag ttgatccgtc cccacctgtc cagacggagc tgccaggccg catcatactg
     5941 gctctgaata aagcgtttat caattaactg tgccagcgtt tcgctctcta tcacgaagcc
     6001 catttcagta ttgagcaatg ttgaacgcgg atcgaaattg aaagagccga taaacaccgt
     6061 tttaccatcg atgctaaaag ttttagcatg caggctggct ccggaattac cggttatgcc
     6121 gcgatcgtgt aacgtactac tttgttcacg cgtcggcttg agttcatata attccacgcc
     6181 atagcggagc aattttttgc gccagcgcgc gtatccggca tggacgacag caacatcgtt
     6241 agcggcaaga gaattggtta ggatcgcaat ctttacccct tttctcacca tccgtaagag
     6301 ttgcgccaca cctgcgcgtg tcggtacaaa ataggaagag ataatatcga tgcgttcact
     6361 gggtgagccc atgatatcgg acaggcgctg cggtaacagt gaatgccgtt ttgccttgcc
     6421 ctcccctttc gccggatcat cacttaataa acgtgttttc gcccagataa gcggcaacgt
     6481 tccatcaacc agatgattta taaacggact ggattccatt tggcgtaagt aacgatgcgt
     6541 catggcatcg ttatgccagg aggcgggtaa ctcgatgcga tccgccattt caccttccgg
     6601 gacatccagc acctgctgta agggtgaaac cgatttgcaa taccagtagc gggcgaaatc
     6661 atcggcaacg tcctctacca cgggtcctat tgccataaca tctaaatccg aaaaaagtgg
     6721 ctcttcccct gccccaaaat aggcatcacc aatatttcgt cctcccacca gggtaaccac
     6781 gccatcgaca gtgaaacttt tattgtgcat acggcgattg agacgggaaa agtcggtgat
     6841 ataaccaagc ggacgcagca agcgaaacga gaaaggatta aaaagccgga cttcaatgcg
     6901 gggatggctg tcaagcaagc gtaaaatatc gtcaagtccg ggcgtattgt tgtcgtccag
     6961 caacaaacgg acgcgaacgc cacgctttgc tgcggctaac agggcggaaa acagtaaccg
     7021 acccgacata tcgtcctgcc agatgtaata ctgaacatcc agcgtatgct ctgccatttc
     7081 ggccagacga taccgggcgg caaacgcatc cagacttttc tccagaggga aaagaccaca
     7141 ctgaccggga tgttgcgaac acagtggcag caccgcgctc gccagccggg gcaaatcatt
     7201 catctccttg ttgggtaagg agtctttcgt agaggtgggc gttttcttca tcataacaga
     7261 caaagtatac ctgttcgggt aaagcgtgac gggtaataaa ttctgaaacg gttttaactg
     7321 cgatttcagc cgctgccgca cgagggtaac cataaacccc agtactgatt gcaggaaaag
     7381 ccactgacgt atagctgttt gccgccacca gtcgcaggct attcagatag gcatcctgca
     7441 aaagctggtc ttcgttttgt tcaccaccac gccagactgg cccgacggtg tgtactacgg
     7501 ctttagcggg aagatcgcct gcaagcgtaa taacggcatg ccccgtaggg caatcgccct
     7561 gctgttgcct gacttttaaa caggcatcca gcagggccgg acccgctgcg cgatgaatgg
     7621 ccccatcgac gccgccgcct cccattaatg acggattagc cgcattcaca atcacatcaa
     7681 cggccagttt ggtaatatca ccctgcacaa catgaatacg cgttttcata acgcctcctt
     7741 acaccttctt gtttcttaag tgtacagcag gctttttggt taaatcgcat ttttctgctc
     7801 agcaacgtgc gttgttcaga aacttagtta tattcgatgt tcattacagc cagctttttc
     7861 tgtttatcaa gatactgcac ttttactgag gctatttgcg cgtttttaac attaccgctc
     7921 attaacgctt tgctggaata ggttttgccc ggaacagaac cattctgtgg gcagttcatt
     7981 tctacgggtg atgactgggt gctgacgtca catgccggtt ccacaatttg gccataaaaa
     8041 tggatcacac ccccttgcgc ggtgcttcct gcgatggcaa taaaaggaaa aaacaaactg
     8101 ccgagcataa gagagtttaa aaaaggtctg aacatttatt ctatttcctc aatgattagt
     8161 catccttgag ggttgtgtta tccatacttt caggttggct gattcgcagc agaccattct
     8221 ctccagattc atcttatgct cgatatttca acaaattaag acttttctga agagggcggc
     8281 cattgttgtg ataaatgaag tgcctgtcca tcagaaacag taacaactat tttcacccga
     8341 tcttccgggg aaatatttaa actcaacttc gtcaaagcaa taggttgatt agcaggcaat
     8401 gagagggtct tttcttgctt cgtctgactt tgccctgaac tgccttcgcg cagggacaat
     8461 atttgtactc tgcacagaca agattgagta agagtgactt caggaataat ggtatacaca
     8521 tccccttgct gggtcgtatt aaaggttatc tggctggaaa gtgccgcaag gagtaataac
     8581 gcattcatat tcttctcccg aaaaaaacag ggcttgcgcc ctgtttctgt aa
//
LOCUS       DAKISA010000150         8401 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.152, whole genome shotgun sequence.
ACCESSION   DAKISA010000150 DAKISA010000000
VERSION     DAKISA010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.152"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(85..900)
                     /gene="sul2"
                     /locus_tag="OVW45_003906"
     CDS             complement(85..900)
                     /gene="sul2"
                     /locus_tag="OVW45_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCV5528242.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2089..2238
                     /locus_tag="OVW45_003907"
     CDS             2089..2238
                     /locus_tag="OVW45_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528243.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(3005..3445)
                     /locus_tag="OVW45_003908"
     CDS             complement(3005..3445)
                     /locus_tag="OVW45_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCV5528244.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            3663..4052
                     /locus_tag="OVW45_003909"
     CDS             3663..4052
                     /locus_tag="OVW45_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528245.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4096..4284
                     /locus_tag="OVW45_003910"
                     /pseudo
     CDS             4096..4284
                     /locus_tag="OVW45_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4251..5450)
                     /gene="tet(A)"
                     /locus_tag="OVW45_003911"
     CDS             complement(4251..5450)
                     /gene="tet(A)"
                     /locus_tag="OVW45_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HCV5528246.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWRN
                     SSNSRCT"
     gene            5556..6206
                     /gene="tetR(A)"
                     /locus_tag="OVW45_003912"
     CDS             5556..6206
                     /gene="tetR(A)"
                     /locus_tag="OVW45_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HCV5528247.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            complement(6238..6480)
                     /locus_tag="OVW45_003913"
     CDS             complement(6238..6480)
                     /locus_tag="OVW45_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HCV5528248.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(6786..7622)
                     /locus_tag="OVW45_003914"
     CDS             complement(6786..7622)
                     /locus_tag="OVW45_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCV5528249.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(7622..>8401)
                     /locus_tag="OVW45_003915"
                     /note="aph(3'')-Ib"
     CDS             complement(7622..>8401)
                     /locus_tag="OVW45_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCV5528250.1"
                     /translation="GESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASRRGELAGER
                     DRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPSMGQQLG
                     AVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARVERELP
                     VRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEE
                     NWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
BASE COUNT         1890 a         2322 c         2333 g         1856 t
ORIGIN      
        1 aaaaaaaata ttagttcgat tcaatggagg ttccttcagt tttctgatga agcgcgaata
       61 tagagaaata tcccgaatgt gcagttaacg aattcttgcg gtttctttca gcgccgccaa
      121 taccgccagc ccgtcgcgca aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc
      181 tgcggcggcg gcaagctctg cagcgagtgt cgcggccccg acatcccccg gaccacggcc
      241 tgtgagcgcg cgcagaaagg atttgcgcga aacagacaga agcaccggca aatcgaagcg
      301 cagccgcaat tcatcgaacc gcgccagcac cgagagcgag gtttcgggag cagcccccag
      361 aaaaaacccc atgccgggat caaggacaag gcggttgcgt ttgataccgg cacccgtcag
      421 cgccgcgatg cgcgcgtcaa agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc
      481 ctcgcgccga tctgcctgcc cgtcttgcac cgaatgcata acgacgagtt tggcagatga
      541 tttcgccaat tgcggataga acgcagcgtc tggaaaaccg cgaatatcat tgagataggc
      601 cacaccacgc gacaaggcat aggcttgcgt cgcgggttga taactgtcga gcgagacggg
      661 aatgccatct gccttgagcg cgtccagcac cggcgcgata cgcgcgattt ctgtgtcgga
      721 cgaaacaggc gcggcgtcgg gattgctgga tgccggaccg aggtcgatca catctgcccc
      781 ctcggccatc agcttacgcg cctgcgcaat ggctgcgtct ggcgccagat accggcctcc
      841 atcggagaaa ctgtccgagg ttatgttgac gatgccgaaa atgatgagcg atttattcat
      901 gggggcttct ataataataa taatcgagca tgagtctcat acggatgctc gggtcgaaag
      961 ggaatcccca ggcgagtaac ctgtttgcgg tgatccgttt catgatgatg tatctgtagc
     1021 attttggaaa taaatatatc atctctcaat aagtagatat atttatcttt tttgcttctt
     1081 tggtgctgca agtcaccaat acgagggaaa cctgttacgg agcggtttaa ccgtaatcaa
     1141 cagtgactgg tgtcatgttc ggaagattaa ttgatagtaa tctacaaagg atcttcttga
     1201 gatccttttc aatgaatcgt aatctcttgc tctgtaaacg aaaaaaccgc ctggggaggc
     1261 ggtttgttcg aaggttaagt cagttgggga actgcttaac ccggtaacag ggttcacgag
     1321 accgccatta ccaaaactgt tctttcagtg tagccgcagt caggcctcta cttcaagacc
     1381 tcttaatcta tcttctcgtg cactcctgtt actaatggct gctgccagtg gcgttttgtc
     1441 gtgtctttcc gggttggact caagttgata gttaccggac aaggcgcagc ggtcggactg
     1501 aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcacgg aactgagtgt
     1561 caggcgtgga atgagaaacc gcggccataa cagcggaatg acaccggtaa accgaaaggc
     1621 aggaacagga gagcgcacga gggagctacc agggggaaac gcctgggatc tttatagtcc
     1681 tgtcgggttt cgccaccact gatttgagcg tcagattttg cgatgcttgt caggggggcg
     1741 gagcctatgg aaaaacggct tcgccgtggc cttatctctt ctttgctaag tgtcttcctg
     1801 gtatcttcca ggaaatttcc gtccccgtcc gtaagccggt accgctcgcc gcagccgaac
     1861 gaccgagcgt agcgagtcag tgagcgagga agcggaatat ctcctgtatc gcatattctg
     1921 ctgacgcgcc ggtgctgcct tttttctcct gccacatgaa gcacttcact gattttcgca
     1981 atcgtggcaa catagtaagc cagtatacac tccgctagcg ctacatgact ggttcagggc
     2041 tgcgccccga aacccgctaa caacctctca gctactggat atcaccccat gccatctgaa
     2101 tatgatgaac aactggattc gactgatacg cagcttcccg gcgaatatga cctttttgat
     2161 ttaatggcgc aggttactga agagaacctg catgcccctg tggattttgg gtcgcctgtc
     2221 ggccgggaag cgttatagct ttttcgcttg cccgcgtacg tggtaatact ccggtattac
     2281 cttttatttc tttattttca tgtaattaca gtggattttt aatacccggg tattactcgt
     2341 gaaaacgaaa tattcctttt ttttctttta attcatgcag ttgcgatgaa aatcgcggct
     2401 gcgttaggtg tatgaccatt ttgaggttga aaaataatca accatggcgg gtgatttacg
     2461 caggttaagg ggagatcgtg acaaaaaaag cgttcctcac tgtatcgttc atgcctgcat
     2521 gaacgggtta ttcttcctga ctacagaatt acatcgctcc ttcagttcat acatcacttt
     2581 tacatcactt atacatcact tatacatcac ttatacatca cttttacatc attccttcag
     2641 ctcatacgtc actttcatcc tctgtgccat ttgtacatca cccccttcct gaacgtgctg
     2701 acagcgggtt ttccggctgc gccggcaacg cttcacggcc acgcaataaa ttgcgtcccc
     2761 gttcaccgtt ttcggatggg aagaacgccg gaaacaggac ttaccccggc tgaatggtgt
     2821 gaatttccgc gctatgcact tgtgcgcata ctcatgcatg ccgtaaaaac agagcctgcg
     2881 cgtttctggc gggttttcgg gtggtttgtt gcctgtttta ccggtttccc gtcagaaacg
     2941 ccctgagggc ctctcaggcg gtgctgataa cagatgttat ggtaaatcag agtcacctgc
     3001 actgttactc tgaaccggag cgaaacagcc gcgaccagaa tccccgcttt ttttcctgtg
     3061 ccagcgcgtc agccagacgg ctaatttcgg cctgtagttg ttccctctcc tcatttcgca
     3121 ggcggttttc tgcttcccgc gccatcttgt cttcaagcat tagggtaaca gtctgctgca
     3181 gagcgtccag tttctgctga atgacgtcta gctgagcatt ttccgcctgg ctaacgacag
     3241 atttggagtg tccctccttg tgcctttttg gtgtctcaga agtgcgcagc tcgccatagg
     3301 cccgaatcag ttcactggtc tcgaactccc ggcgatcatt ttttcctgtg cgatagctga
     3361 caaggccttt agccatgtca cgatataatt gcgagcgcga ctttcctgtt aatttcagtg
     3421 tctcttgaac tgtgtgccat gccataaaag tgacagtgtc tcctgtgtgt accgtattgt
     3481 aacagcagaa catattactg ttttaacaca aaaggtctga ggctgcccgt gaggcgtgaa
     3541 aaatacacaa aaaacctgtt gacggtgtgg ttttgacggg cgtagtattt cgaatactgt
     3601 atataaacac attgttataa atacagtttt tgctgttttt aaaagtcaaa gaggataatt
     3661 ttatgagtac agagttaaat agcggtactg tcggtggttt tggtcacatg ggaccaaatg
     3721 acccctacaa tgatggtcat agcggctctg gaagttcggg aggtagtttt aacggtggtg
     3781 gtaatggtgg ccatgataat agtagtggtt catcttatac tgcatccttt ggtctcatca
     3841 ctggccgaca cccgataact gtcccaggta cgaagaatat taattctgtc gatctttctg
     3901 ctgtcattaa taacattata catcatgaag gtgcacgcaa taacatgtac ctagatactg
     3961 cgaacaatgt aagagtggga gtgacgggca ctggctggca atgtctagca acggcaggca
     4021 tttcggctga gggtaaaaga actttccgct aagcgataga ctgtatgtaa acacagtatt
     4081 gcaaggacgc ggaacatgcc tcatgtggcg gccaggacgg ccagccggga tcgggatact
     4141 ggtcgttacc agagccaccg acccgagcaa acccttctct atcagatcgt tgacgagtat
     4201 tacccggcat tcgctgcgct tatggcagag cagggaaagg aattgccggg ctatgtgcaa
     4261 cgggaatttg aagaatttct ccaaagcccg cgacgcagcg ccggcaggca gagcaagtag
     4321 agggcagcgc ctgcaatcca tgcccacccg ttccacgttg ttatagaagc cgcatagatc
     4381 gccgtgaaga ggaggggtcc gacgatcgag gtcaggctgg tgagcgccgc cagtgagcct
     4441 tgcagctgcc cctgacgttc ctcatccacc tgcctggaca acattgcttg cagcgccggc
     4501 attccgatgc cacccgaagc aagcaggacc atgatcggga acgccatcca tccccgtgtc
     4561 gcgaaggcaa gcaggatgta gcctgtgccg tcggcaatca ttccgagcat gagtgcccgc
     4621 ctttcgccga gccgggcggc tacagggccg gtgatcattg cctgggcgag tgaatgcaga
     4681 atgccaaatg cggcaagcga aatgccgatc gtggtcgcgt cccagtgaaa gcgatcctcg
     4741 ccgaaaatga cccaaagcgc ggccggcacc tgtccgacaa gttgcatgat gaagaagacc
     4801 gccatcaggg cggcgacgac ggtcatgccc cgggcccacc ggaacgaagc gagcgggttg
     4861 agagcctccc ggcgtaacgg ccggcgttcg cctttgtgcg actccggcaa aaggaaacag
     4921 cccgtcagga aattgaggcc gttcaaggct gccgcggcga agaacggagc gtggggggag
     4981 aaaccgccca tcagcccacc gagcacaggt cccgcgacca tcccgaaccc gaaacaggcg
     5041 ctcatgaagc cgaagtgccg cgcgcgctca tcgccatcag tgatatcggc aatataagcg
     5101 ccggctaccg ccccagtcgc cccggtgatg ccggccacga tccgcccgat atagagaacc
     5161 caaaggaaag gcgccgtcgc catgatggcg tagtcgacag cagcgccggc cagcgagacg
     5221 agcaagaccg gccgccgccc gaaacgatcc gacagcgcgc ccagcacagg tgcgcaggca
     5281 aattgcatca acgcatacag cgccagcaga atgccatagt gggcggtgac gtcgttcgag
     5341 tgaaccagat cgcgcaggag gcccggcagc accggcataa tcaggccgat gccgacagcg
     5401 tcgagcgcga cagtgctcag aattacgatc aggggtctgt tgggtttcac gtctggcctc
     5461 cggaccagcc tccgctggtc cgattgaacg cgcggattct ttatcactga taagttggtg
     5521 gacatattat gtttatcagt gataaagtgt caagcatgac aaagttgcag ccgaatacag
     5581 tgatccgtgc cgccctggac ctgttgaacg aggtcggcgt agacggtctg acgacacgca
     5641 aactggcgga acggttgggg gttcagcagc cggcgcttta ctggcacttc aggaacaagc
     5701 gggcgctgct cgacgcactg gccgaagcca tgctggcgga gaatcatacg cattcggtgc
     5761 cgagagccga cgacgactgg cgctcatttc tgatcgggaa tgcccgcagc ttcaggcagg
     5821 cgctgctcgc ctaccgcgat ggcgcgcgca tccatgccgg cacgcgaccg ggcgcaccgc
     5881 agatggaaac ggccgacgcg cagcttcgct tcctctgcga ggcgggtttt tcggccgggg
     5941 acgccgtcaa tgcgctgatg acaatcagct acttcactgt tggggccgtg cttgaggagc
     6001 aggccggcga cagcgatgcc ggcgagcgcg gcggcaccgt tgaacaggct ccgctctcgc
     6061 cgctgttgcg ggccgcgata gacgccttcg acgaagccgg tccggacgca gcgttcgagc
     6121 agggactcgc ggtgattgtc gatggattgg cgaaaaggag gctcgttgtc aggaacgttg
     6181 aaggaccgag aaagggtgac gattgatcag gaccgctgcc ggagcgcaac ccactcacta
     6241 cagcagagcc atgtagacaa catcccctcc ccctttccac cgcgtcagac gcccgtagca
     6301 gcccgctacg ggctttttca tgccctgccc tagcgtccaa gcctcacggc cgcgctcggc
     6361 ctctctggcg gccttctggc gctcctgctg cggcgtccgc tcgtgggccg tggcgcgggt
     6421 ccgcgcgccg gcctcgtgcg cctggcgctc gcgggcgagg tccagggcgg ccgtcttcac
     6481 gttctgcctt gcgcagatga gatagatcta gcgtggactc aaggctctcg cgaatggctc
     6541 gcgttggaaa ctttcattga cacttgaggg gcaccgcagg gaaattctcg tccttgcgag
     6601 aaccggctat gtcgtgctgc gcatcgagcc tgcgcccttg gcttgtctcg cccctctccg
     6661 cgtcgctacg gggcttccag gggtcgtttg cgggaagggg cggaatccta cgctaaggct
     6721 ttggccagcg atattctccg gtgagattga tgtgttccca ggggatagga gaagtcgctt
     6781 gatatctagt atgacgtctg tcgcacctgc ttgatcgcgg ccgcgatagc tagatcgcgt
     6841 tgctcctctt ctccatccgc gttccaagct gcggaaaggc acccataagc gtacgcctgg
     6901 tcgagcaggc gacgcggatc gacgtccagc gcacgagaga atgcgtccgc catctgtgca
     6961 atgcgtctag gatcgagaca aaggtcgtct ctgtcagccg gatcgtagaa catattggcg
     7021 gcgccaaagc ccacttcacc gaccagaccg acgggatcta tcaccagcca gccgcgactg
     7081 gagaacatga tgttttcatg atgcagatcg ccatgtagcc cacgcagttc cgaggcattg
     7141 ctcatcattt gatcggctat aatcgccgcg tggacgtagt cagtttgaca acctgcgttt
     7201 tgatcatcgc gcgcccgctg aaacaaagct gcaaagcgat cccggatcgg gagaagggca
     7261 gaaggcaggg gttcctcaga tgcggcatac agcttcgcca ttagttccgc tgcaatttcg
     7321 gtcgcctggt agtcgccgtg ctcggcaacg atgtgagaga gcattcgctc cccggcatat
     7381 tcgagcaaca tcagattgtt ctcacgaccg agcaaccgga ctgctcccct cccattgcgc
     7441 cataccagat agtcggcccc gcgcagttca tcagcaatgt cttctatagg tttcaatccc
     7501 ttgacgattg caggagtccc gtctggcaat gaaactttcc aaacgaggct ggaaaaggtg
     7561 tccgcaatga gaacaggttg cgaaacgtgc caatgagcag gaaaaacagg cggcatgaac
     7621 atcaacccca agtcagaggg tccaatcgca gatagaaggc aaggcgttcg cggtcggggg
     7681 cttcgatccc caatacattg aataggacag cgaaggcgcg ctctgcttca tctggcgctg
     7741 cccagttctc ttcggcgtta gcaatcatga gtgccaaatc ggcatagcga tctgctgttc
     7801 cgagccgccc aaggtcgatc agacccgtgc attgaagagt tttagggtcc accatgaagt
     7861 tcggcatgca gggatcacca tggcaaacaa ccatatcggt gcgctcttgg tcgagccgca
     7921 ccggtagctc tcgttcgaca cgagccaaaa gatcgagctg cggcgtactc ttgtcctcgt
     7981 ccggtaagaa gtcgggattg acggcattgc gggacaccac atcaacggcg cgtccgaaca
     8041 ttcgcgacag cctgcgctca aacggacatt gatcaaccga taggctgtga acagcgccaa
     8101 gttgctgccc cattgacggc cacgctttga gcaaatccgc tccagacaga tcagccgccg
     8161 gtactcccgg aattgccgtt atcaccaagc atgcaccctc ctgttcctcc tgccagttga
     8221 tgacctcggg gcaagccaca cctcgacctt tgagccaaat gaggcggtca cgctctccag
     8281 cgagctcacc gcggcgggaa gcaggtgcga ttttcgcgaa ggcatgcccg tcaccacgtc
     8341 gaaaaacaaa atcaccagat tctccgcctc tgacaggcaa ccagtcagaa tgcgattcac
     8401 c
//
LOCUS       DAKISA010000151         8213 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.153, whole genome shotgun sequence.
ACCESSION   DAKISA010000151 DAKISA010000000
VERSION     DAKISA010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8213
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.153"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(215..1105)
                     /gene="dgcZ"
                     /locus_tag="OVW45_003916"
     CDS             complement(215..1105)
                     /gene="dgcZ"
                     /locus_tag="OVW45_003916"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HCV5528251.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTDPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(1360..1752)
                     /gene="ydeI"
                     /locus_tag="OVW45_003917"
     CDS             complement(1360..1752)
                     /gene="ydeI"
                     /locus_tag="OVW45_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HCV5528252.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            2028..2546
                     /locus_tag="OVW45_003918"
     CDS             2028..2546
                     /locus_tag="OVW45_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCV5528253.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(2591..4636)
                     /gene="dcp"
                     /locus_tag="OVW45_003919"
     CDS             complement(2591..4636)
                     /gene="dcp"
                     /locus_tag="OVW45_003919"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCV5528254.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            4773..5519
                     /gene="ydfG"
                     /locus_tag="OVW45_003920"
     CDS             4773..5519
                     /gene="ydfG"
                     /locus_tag="OVW45_003920"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCV5528255.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRPERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            5608..6294
                     /gene="ydfH"
                     /locus_tag="OVW45_003921"
     CDS             5608..6294
                     /gene="ydfH"
                     /locus_tag="OVW45_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCV5528256.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            6471..6674
                     /gene="ydfZ"
                     /locus_tag="OVW45_003922"
     CDS             6471..6674
                     /gene="ydfZ"
                     /locus_tag="OVW45_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCV5528257.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(6710..8170)
                     /locus_tag="OVW45_003923"
     CDS             complement(6710..8170)
                     /locus_tag="OVW45_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCV5528258.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
BASE COUNT         2168 a         2056 c         1871 g         2118 t
ORIGIN      
        1 cggagataat ccctcaccta accggcccct tgttacagtt gtgtacaagg ggcctgattt
       61 ttatgacggc gaaaaaaaac cgccagtaaa ccggcggtga atgcttgcat ggatagattt
      121 gtgttttgct tttacgctaa caggcatttt cctgcactga taacggatcg ttgacacagt
      181 agcatcagtt ttctcaatga atgttaaacg gagcttaaac tcggttaatc acattttgtt
      241 cgtcaataaa catgcagcga tttcttccgg tttgcttacc ctcatacatt gcccggtctg
      301 ctcttccaat gaccacatcc agaggctctt caggaaatgc gcgactcaca cctgctgtca
      361 cggtaatgtt gatatgccct tcagaatgtg tgatggcatg gttatcgact aactggcaaa
      421 ttctgacacc tgcacgacat gcttcttcat cattagccgc tttaacaata atgataaatt
      481 cttcgccccc gtagcgataa accgtttcgt aatcacgcgt ccaactggct aagtaagttg
      541 ccagggtgcg taatactaca tcgccgatta aatgcccgta ggtatcatta accaatttaa
      601 atcggtcaat atccaacaac attaaataaa gattcagagg atcagtgttg cgtaactgat
      661 gatcaaagga ttcatcaaga acccgacgcc ccggcaatcc cgtcaaaaca tccatattgc
      721 tacggatcgt cagcaaataa attttgtaat cggttaatgc cgcagtaaaa gaaagcaacc
      781 cctcctgaaa ggcgtcgaaa tgcgcgtcct gccagtgatt ttcaacaata gccagcatta
      841 attcccgacc acagttatgc atatgttgat gggcagaatc cattagccga acgtaaggta
      901 attcatcgtt atcgagtggc cccagatgat caatccaccg accaaactgg cacagtccat
      961 aagaatggtt atccgttatt tctggcttac tggcatctct cgcgaccacg ctgtgaaaca
     1021 tactcaccag ccactggtag tgggcatcga tagccttatt gagatttaac aagatggcat
     1081 caatttccgt tgtcttcttg atcattgcca ctcctttttc acagttcctt gtgcgcgcta
     1141 ttctaacgag agaaaagcaa aattacgtca atattttcat agaaatccga agttatgagt
     1201 catctctgag ataacattgt gatttaaaac aaaatcagcg gataaaaaag tgtttaattc
     1261 tgtaaatcac ctctgcatta tcgtaaataa aaggatgaca aatagcataa cccaataccc
     1321 taatggccca gtagttcagg ccatcaggct aatttatttt tatttctgca aatgagtgac
     1381 ccgaacgacg gccggcgcgc ttttcttatc cagactgccg ctaatgttga tcatctggtc
     1441 cggctgaact tctcgtccat caaagacggc cgcaggaata acaacattaa tttcaccgct
     1501 cttatcgcga aaaacgtaac ggtcctctcc tttgtgagaa atcaaattac cgcgtagtga
     1561 aaccgaagcg ccatcgtgca tgttttttgc gaaatcaacg gtcatttttt ttgcatcatc
     1621 ggttccgcga tagccatctt ctattgcatg aggcggcggt ggcgctgcat cctgttttaa
     1681 accgccctgg tcatctgcca acgcataagg catgacaaga aaacttgcta atacaatgga
     1741 ctgaaatttc atactaactc cttaattgcg tttggtttga cttattaagt ctggttgcta
     1801 tttttataat tgccaaataa gaatactgcc aattgttata aggcatttaa aatcagccca
     1861 ctagctgtca aatattcaga gaatttgact cactaaagtt aagaagattg aagagtctta
     1921 aacatgtttt cagaataatc ggatttatat gtttgaaaat tattatattg gacgagcata
     1981 cagaaaaagc aaatcacctt tacatataat agcgtggaca aaaaacagtg aacattaata
     2041 aagataaaat tgtacaactc gcagataccg acactattga aaacctgaca tccgcgttga
     2101 gtcaaagact tatcgcggat caattacgct taactaccgc cgaatcatgc accggcggta
     2161 agttggctag cgccctgtgt gcagctgaag atacacccaa attttacggt gcaggctttg
     2221 ttactttcac cgatcaggca aagatgaaaa tcctcagcgt aagccagcag tctcttgaac
     2281 gatattctgc ggtgagtgag aaagtggcag cagaaatggc aacaggtgcc atagagcgtg
     2341 cggatgctga tgtcagtatt gccattaccg gctacggcgg accggagggc ggtgaagatg
     2401 gtacgccagc gggtaccgtc tggttcgcgt ggcatattaa aggccagaac tacactgcgg
     2461 ttatgcattt tgctggcgac tgcgaaacgg tattagcttt agcggtgagg tttgccctcg
     2521 cccagctgct gcaattactg ctataaccag gctggcctgg cgatatcccc aggccagcca
     2581 ttggtggtgt ttatatgttc aagccacgat gttgcagcat cggcataatc tgaggagcct
     2641 taccgcgcca ttgtcgatgc aggcgttcca gatcttcgct gttacctctg gaaaggatcg
     2701 cctcgcgaaa acgctgccca ttttcacgcg ttaatccgcc ctgctcaaca aaccactggt
     2761 aaccatcatc ggccaacatt tgcgtccaca gataagcgta ataacctgca gcatatccgc
     2821 caccaaaaat atgggcgaaa taactgctgc gatagcgtgg cggtatagca ggaagatcca
     2881 tattttccgc caccagcgcc cgcaattcaa aatcatcgac atcctgcatt gcttcgtttt
     2941 cttccaggca atgccagcgc atatcgagaa gtgcggcgct aagcagttcg ctcatctcat
     3001 accctttgtt gaacaggctg gcattacgca ttttctgttg cagttcgtca ggcattgctg
     3061 ccccgctctg ataatgccgg gcgtagcgag cgaatacctg cggatgcgtt gcccagtgtt
     3121 cgttgatttg cgacggaaat tcgacaaaat cacgcggcgt gttggtgccg gaaagcgtgg
     3181 cataacgctg gcgggcaaaa aggccgtgca gcgtatgacc aaattcatgg aataaggtta
     3241 ttacatcatc ccagagtaac aacgcaggct caccggcagc gggtttctga taattgcaga
     3301 cgttataaat taccggatgc gtttcattaa gcgttgattg ctcaacaaaa ttgcccatcc
     3361 atgcaccgcc gctttttgaa tcacgggcga agaaatcacc gtaaaataac gccagcccca
     3421 cgccattatg atcaaaaatt tcccacaccc gaacgtcagg atggtagaca ggaatatcaa
     3481 aacgttcgac aaacttaata ccgaagagct gattcgcggt ccagaataca ccttcattca
     3541 acaccgtgtt taattcaaaa tatggcttga gctgcgcttc atcaagatca aatttctccc
     3601 gccgtacctg ttccgcataa aatgcccagt cccacggctg cgcgctaaac ccgccctgct
     3661 gcttatcgat aaccgcctgt atggaggcta attcatcgct cgcacgttga cgcgccgctg
     3721 gaacaatttc ccgcataaag ttgagtgctg cttctggcgt ttttgccatc tgatcggcga
     3781 ttttccatgc ggcataatga ggaaaaccaa gcagtttcgc ctgctgtgcg cgaatctcta
     3841 ccagacgttg aatgatagcg cgggtatcat tgccatcatt tttttccgct cgcgtccagc
     3901 ccgcaataaa cagtttttca cgcgtcgcac gatcgcgcat ttcggcaagc gccggttgtt
     3961 gggtggtatt cagcagcgga atcagccatt tgttatccag acctttctcg cgagccgcct
     4021 ctgccgccag cgcaatctct tgctcactca ttcctgccag ctgcgcgata tcgttcacaa
     4081 ccagaccgcc ggatttattt gctgccagta atcgctggtt aaactggctg gtcagggtcg
     4141 cagcttctgt attcagtact tttaattttg ctttatcagc ttgcgcaagt ttggctccgg
     4201 caaggacaaa acgttgatga atcacctcca ccaggcggat ggattcacta tcaagcccca
     4261 gggattcacg gcgctgccag acagcatcta cccgcgcgaa taattcaccg ttcagataga
     4321 tatcattagc cagttccgcc agttcagcgg aaaactgttc atcaagacgc tgtaattcat
     4381 cattggtatg cgccgcagtc atcgcaaaaa agacgctggt aacgcgggta agtaattctc
     4441 cgctttgttc cagtgccaga atagtattgt tgaaatcagg cgtttgcggg ttaagcgcga
     4501 tggcagcaat ttctgcccgc ttttgctgca ttccctcatc gaatgccggg cgatagtgat
     4561 gattggcaat ttgatcaaaa tggggagcca gatacggtag tgtgctttgc acaaggaaag
     4621 gattcattgt tgtcattttc ttctcctgaa cgcgagatgt tccatagcgt aggcttactg
     4681 ataacggagt gcaatcttgc aaaccagtat tacccgctct taagcatcac gtgctatgtt
     4741 attgacacac aaaagcgttg aggaacagtg agatgatcgt tttagtaact ggagcaacgg
     4801 caggttttgg tgaatgcatt actcgtcgtt ttattcaaca agggcataaa gttatcgcca
     4861 ctggccgtcg cccggagcgg ttgcaggagt taaaagacga actgggagat aatctgtata
     4921 tcgcccaact ggacgttcgc aaccgcgccg ctattgaaga gatgctggca tcgcttcctg
     4981 ccgagtggtg caatattgat atcctggtaa ataatgccgg cctggcgttg ggcatggagc
     5041 ctgcgcataa agccagcgtt gaagactggg aaacgatgat tgataccaac aacaaaggcc
     5101 tggtatatat gacgcgcgcc gtcttaccgg gtatggttga acgtaatcat ggtcatatta
     5161 ttaacattgg ctcaacggca ggtagctggc cgtatgccgg tggtaacgtt tacggtgcga
     5221 cgaaagcgtt tgttcgtcag tttagcctga atctgcgtac ggacctgcat ggtacggcgg
     5281 tgcgcgtcac cgacatcgaa ccgggtctgg tgggtggcac cgagttttcc aatgtccgct
     5341 ttaaaggcga tgacggtaaa gcggaaaaaa cctatcaaaa taccgttgca ttgacgccag
     5401 aagatgtcag cgaagccgtc tggtgggtgt caacgctgcc tgctcacgtc aatatcaata
     5461 ccctggaaat gatgccggtt acccaaagct atgccggact gaatgtccac cgtcagtaat
     5521 ttttataccc ggcgtaactg ccgggttatt gcttgtcaca aaaaagtggt agactcatgc
     5581 agttaactca ctcacaagca agaacgaatg accgtcgaaa cgcaacttaa tcccacacag
     5641 cctgtcaatc agcaaattta tcgtattctt cgtcgcgaca ttgtccattg cctgattgca
     5701 ccaggcacac cgttgtcgga aaaagaagtt tctgttcgtt tcaatgtgtc acgccagccg
     5761 gttcgtgaag cctttattaa actggcggaa aacggcctga ttcaaattcg tccgcagcgt
     5821 ggcagctacg tcaacaaaat ttccatggcc caggtgcgca atggcagttt tatccgtcag
     5881 gccattgagt gcgcggtggc gcgtcgggcg gcgagcatga ttaccgaaag ccagtgctac
     5941 caactggaac aaaatcttca ccagcaacgc attgccattg agcgcaagca actggatgat
     6001 ttttttgaac ttgatgacaa cttccatcaa ctcctgacgc agattgccga ctgtcaactg
     6061 gcgtgggata ccattgagaa cctgaaagcg accgttgatc gcgtgcgcta tatgagtttc
     6121 gaccacgttt ctccaccaga aatgctgtta cgccagcatc ttgatatttt ctctgccctg
     6181 caaaaacgtg atggcgatgc ggtagaacgt gcaatgacgc aacatttgca ggaaatcagc
     6241 gaatccgtgc gccagatccg ccaggaaaat agcgactggt ttagcgaaga gtaattcatt
     6301 tcctctcatc ccatccgggg tgagagtctt ttcccccgcc ttatggctca tgcatgtatc
     6361 aaaaaagatg tgagcttgat caaaaacaaa aaatatttca ctcgacagga gtatttatat
     6421 tgcgcccgtt acgtgggctt cgactgtaaa tcagaaagga gaaaatacct atgacgacct
     6481 acgatcgtaa ccgtaacgca atcaccactg gcagccgtgt tatggttagc ggcaccggtc
     6541 acactggcaa gatcctgtcg attgatactg aaggtctgac cgctgagcaa atccgccgcg
     6601 gaaaaaccgt agttgttgaa ggttgtgaag agaaactggc acctctggac ctgattcgtc
     6661 tcggcatgaa ctaagcgtgt gaatgccgcc gatggcggca ttgctttttt tacttcacgg
     6721 aatatttcgc cacggtcgct ttcgcgccat gcgctaataa agacaagtac gcttccgtca
     6781 cttttgcagt aaacaaacta ttgtctggca aatcatcacc aaagatcgcc ttaatcgcca
     6841 gcaatgactg gacgcgcgct ttcccttcag cactactttg tacagccttc tgaataacag
     6901 gtaacagtgg gtcactgatt tcaatcggat ttccctgttc atcaacacca ccgacataac
     6961 gcatccaacc cgcgacgccc agtgccagca gatcgaactt gctgtcatgc gccagatgcc
     7021 agcgaacaga atccaacatc cgctgtggca atttctggct gccatccatc gcaatctgcc
     7081 aggttcgatg acgtaacgcc gggttgctat agcgttcaat taatcggtta gcgtaatctt
     7141 gcaaatcaac gccctgcact ttcagcgtcg gcgcttgttc ctgcaacatc aagccatacg
     7201 ccgcataacg ataatgttca tcttccatgc agtcattaat gtgctgatat ccagcaagat
     7261 aacccaaata cgccaggaat gaatgactgc cgttgagcat gcgcaacttc atctcttcat
     7321 aaggcagcac atcactaacc agttcggctc ccgctttttc ccattctgga cgtccggcaa
     7381 caaagttatc ttctattacc cactggcgga aaggttcaca ggcaacgccc gcaggatcgc
     7441 ccacaccggt aagttgttcg attttcgcca gcgtatcctc tgtcactgcg ggcacaatac
     7501 ggtccaccat tgttgatggg aaagtcacgt tatcttcgat ccattgtgcc agttttacat
     7561 caacagcttt cgcgtatgaa gtgacaacgt cacgcataac atgaccgttt tctggcatgt
     7621 tgtcacatga catgacggta aatgcgggaa gtcctgccgc tttacggcgg gccagcgcct
     7681 caacaatcac ccctgttgct gttttcggct ggtggggatt ttgcacgtcg gcagcgacca
     7741 tcgggtgatc gagcattaac tgtccggtcg ccggagagtg gaaataccct ttttcggtga
     7801 ttgtcagaga gacaatcgcg atttgcggtt cacacatcgc agccaacacg gtttctaagc
     7861 catccatctg tacgtgcaag gctttcttaa cgacgccaac gacgcgagcc gtccacgcat
     7921 cggccgacat ttccgcaacg gtataaagat tatcttgctg ttgtaaatcg gcaatttgct
     7981 gttcgccgcc gattaagttg acctcataat atccccagtc actgaaatgt tctgtagcaa
     8041 gaatatcggc atacacgccc tgatgcgcac ggtgaaatgc accaaagcct aaatgaataa
     8101 ttcttggggc caggttatta cgatcataaa cagggagtgt cgcttttgct gataacaaat
     8161 tatttcccat aacaattcct taaatataaa tatggcaagc tatatgtttt gtt
//
LOCUS       DAKISA010000152         8212 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.154, whole genome shotgun sequence.
ACCESSION   DAKISA010000152 DAKISA010000000
VERSION     DAKISA010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8212
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.154"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..311
                     /locus_tag="OVW45_003924"
     CDS             <1..311
                     /locus_tag="OVW45_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528259.1"
                     /translation="LNIPRWPASVSRKVILPYIRGEVELVRIRDAEGRIAAEGALPYP
                     PGVLCVVPGEVWGGAVQRYFLALEEGVNLLPGFSPELQGVYSETDADGMKRLYGYVLK
                     "
     gene            complement(360..1616)
                     /gene="nupG"
                     /locus_tag="OVW45_003925"
     CDS             complement(360..1616)
                     /gene="nupG"
                     /locus_tag="OVW45_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCV5528260.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(1818..2897)
                     /gene="mltC"
                     /locus_tag="OVW45_003926"
     CDS             complement(1818..2897)
                     /gene="mltC"
                     /locus_tag="OVW45_003926"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCV5528261.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(2962..3237)
                     /locus_tag="OVW45_003927"
     CDS             complement(2962..3237)
                     /locus_tag="OVW45_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCV5528262.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(3265..4317)
                     /gene="mutY"
                     /locus_tag="OVW45_003928"
     CDS             complement(3265..4317)
                     /gene="mutY"
                     /locus_tag="OVW45_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCV5528263.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            4478..5197
                     /gene="trmB"
                     /locus_tag="OVW45_003929"
     CDS             4478..5197
                     /gene="trmB"
                     /locus_tag="OVW45_003929"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCV5528264.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            5197..5523
                     /locus_tag="OVW45_003930"
     CDS             5197..5523
                     /locus_tag="OVW45_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCV5528265.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            5707..6426
                     /locus_tag="OVW45_003931"
     CDS             5707..6426
                     /locus_tag="OVW45_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCV5528266.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            6602..7648
                     /gene="ansB"
                     /locus_tag="OVW45_003932"
     CDS             6602..7648
                     /gene="ansB"
                     /locus_tag="OVW45_003932"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCV5528267.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            7765..>8212
                     /locus_tag="OVW45_003933"
     CDS             7765..>8212
                     /locus_tag="OVW45_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCV5528268.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFN"
BASE COUNT         2101 a         2005 c         2171 g         1935 t
ORIGIN      
        1 cgttaaatat cccacgttgg ccagcatctg tctcacggaa agtcatactg ccttatattc
       61 gcggtgaagt ggagttggtg cggattcgtg atgccgaagg gcgaattgcg gcagaagggg
      121 cgttgcctta tccacctggc gtgctttgcg tggttcccgg ggaagtctgg ggtggggcgg
      181 ttcaacgtta tttccttgcg ctggaagaag gcgtgaattt gttgccggga ttttcgccgg
      241 agttgcaagg tgtctatagc gaaaccgatg cggatggcat gaaacgattg tacggttatg
      301 tgttgaagta agaataaaaa aacgggtcac cttctggcga cccgtttttc tttgcgtaat
      361 tagtggctaa ccgtctgtgt gcctgtcggg acacgaacgt gtttatattt gaacatcgcc
      421 atgaacgcga aggccagaac cacggaataa ccagcgaaaa tcaaccatac ggtctgccag
      481 tcggtaatgc cgttttgggt gtacatctca acaactttac cgctcacgat gccgccgagg
      541 atacagccga agccgttagt catcatcagg aacatccctt gtgcactggc gcgaattgcc
      601 gggctaactt ctttttcgac aaacaccgaa ccagagatgt tgaagaagtc gaatgcgcaa
      661 ccgtaaacga tcatcgacag taccagcagt acagtaccga acggagtcgg gtcgccgtaa
      721 gcaaacagcg caaaacgcag gatccacgcc acaatactga tcatcattac gttcttaata
      781 ccgtagcggc ttaagaagaa cgggatggtc agaatgaaca gggtttcaga gatctgcgaa
      841 atcgacatga tgattgacgc atgctgcacg atgaagctgc tggcaaacat cggatctttg
      901 tcgaagctgt gcaggaaggt attaccgaac atgttggtaa tctgcagttc cgcgcccagc
      961 agcattgaga agatgaagaa gattgccata cgcttgtttt taaacagcgc gaatgcatcg
     1021 aggcccagca gggttgtcca gctctgattc gcttgctgtt tagcaaccgg aatatgcggc
     1081 agagtcaggg taaacagaac cagaatggcg gaaagtgctg cgccaatata cagctgcatg
     1141 tggcttaatt cgaagccaga caggctcacc acccacattg ccatgataaa gccgatggtg
     1201 ccccagatac ggattggcgg gaagtcagta acgatatcca tcccggcatt ttgcaggcga
     1261 tagtaagaga tggtgttgat taacccaagc gttggcatat aagcaaacga gttaatcaat
     1321 atcacaagga acatcgcttc cggcgtcgtg acctgtgccg ccatgaacag cgtgatagcg
     1381 ccaatggtgt ggcaaatggc atatacccat ttcgcactta accatttgtc ggccacaatc
     1441 cccagcagcg caggcataaa taccgcggcg atacccagtg agctataaac tgcgccaata
     1501 gaagcaccgt caaacttcag ggtaacaaac atataggagc cgagggtcgt cagccaactt
     1561 ccccacagac agaactgcag aaaagagagg attttcagct gcagcttaag attcatgtta
     1621 atttcctcac atcgtgatgc ggatgagtgt ttgaaaaagc acatttgacc gtgcgagatg
     1681 atgggattct atcaacttcg cggactggtt ttttgttacc tgtggcaaat aattgcaaac
     1741 attcccaatt tgcaaaacga aattgtgatg tggataacat ttttgcccct gagcatcgtc
     1801 aggggcggtt aatggaatta tcggcggcgg taggattttt gcgcggtatt cactttataa
     1861 agataacggc gagattccgc agagggatgg cgggtcgtca gcgtctgata aacatcgccc
     1921 ggcgtcatgg tgttaataat attggcagcc tgaatcttat cattcgaaaa gactcgcagc
     1981 acgctgcctg cgccgccgtt ataggcggtg atgacggcat aacgccgcga tgttgggtta
     2041 tcaattccgc cgagataaac attgttcagc atcgccagat acgcggtgcc ggtatcaata
     2101 ttgctggcag gatcaaacaa gaaactgcgg ctcggcgtgc cggatttccc ctgcgagcgg
     2161 aacacatctt tcccggcagt atgttgtacc acctgcatta atcccagcgc atcggaacgg
     2221 ctgaccgcat acgggttaaa ggaagattcg gtctgcataa ttgccagaat cagcgactca
     2281 tcaacgccat atttccgtga cgcctggcgg accatgccga gatatttgtg cgcacgttta
     2341 tcaaggtggt tcggtaccat gttaatggtg acgctgtaga tgatacgcag cccgttactg
     2401 cggctttgca gacggttttt cagcagataa tccgcgaagt tgcttgcgcg accttcccag
     2461 cgaatcggct gcccggtgtt gtccaccacc tgaccgtaaa ggaaaggttc tttcgaaatc
     2521 gtaatatcat caacgtcgga atagagatcg accgaactcg gatcgtcacc catcagcaac
     2581 gttttgataa ttgcccggcg caaatgcgcg gcaggttctg tcccggcgat agtttcgata
     2641 gtaatcgtac cgtcatcgaa gttgatgtgg ctgcgggtct gatattgatc ggtgtatttc
     2701 acgtagtcct taggaccggc gatcaccacc tctttgaatc cccagatgtt ctcaatattg
     2761 tgggcaaatt gccccatcag aatatcaaaa ccgttggtat ctttgaccca ggcttcgtta
     2821 taggtatcgc cttttttggt cgtcgaacag gagatgagca acggcgcaat caaagccagc
     2881 gcgagatatt ttttcatcat tccgggtgcg tgttgtgttt gtttttatgc aagttgccgg
     2941 aggcgtgctc cggcactgtt tttatttttt atcttccggc gtatagccct cgatatgcac
     3001 ctctttaccc tcgaacagga agttgaccat ctcctgctca agcagcttgc ggtgctcggc
     3061 attcatcatg ttgagtttct tttcattaat cagcatggtt tgcttgtgct gccactgcgc
     3121 ccaggcttct ttggagatct cgttatagat gcgttttccc agctcgccgg ggtacagctg
     3181 aaaatcctga ccttctgctt cacgttgcag gaaagtacaa aaaatcgttc tgctcataaa
     3241 tcatcctctt tatcgactca cgcgctaaac cggcgcgcca gtgcgtaact gctgtaacaa
     3301 acgctccacg ggagccgcca ggccaactga cggcggttgc gctaagttat accagagcgc
     3361 attgccttca tccatgcagc cggtgaatga cgacacggga agccacatag gcacaatatc
     3421 taagtggaaa tggctgaagg tatgccgaaa cgcggtcagt tgcgtcaggt tatcggcagc
     3481 aatctgccgt tgcgccagcc actgccgcaa actttcttcg tcggcaaact gcgggaaaca
     3541 gtataaaccg ccccacaatc cgctcggcgg acgctgcgcc agcaatactt catcttcgtg
     3601 ctgtaacagc aaaaagtagc cggtgcgctc cggcagcgtc tgtttcggtt ttttgcccgg
     3661 ataaagcgac cagctattat tggcggcggc aatacatccg ttttgtagcg gacagagcga
     3721 acatttcggc ttcgagcgcg tacaaatcat cgcgcccaaa tccatcatcg cctgattaaa
     3781 ccgttccacg ccgaccgcgg gcgtcacctg ctcgctcaaa ctccataatt tattctcgac
     3841 ctcttttttc ccaggccagc cgcttacagc atagcagcgc gccagcacgc gtttgacgtt
     3901 accgtcgaga atcggaaagt gcttacccag agaaagcgag agaatcgcgc ctgcggtgga
     3961 acgcccgacg cccggtaacg ccgcgacttc ttcaaaggtt tccgggaatt taccgccgtg
     4021 taaggtcgcc acttgttgtg ccgctttatg cagattgcgc gcgcgggcgt aatagccaag
     4081 cccggtccac aagtggagaa cttcgtcgag cggcgcattg gcgagatcgg tcaccgtcgg
     4141 gaagcgcgcc ataaagcgtt caaaataggg gataacggtc gcaacctgag tttgttgcaa
     4201 catcacttct gagagccata ctttgtaggg cgtcttgtca atttgccagg gcagcgtttt
     4261 tcgcccgtat ttatcgtacc agtccagaac ctgggctgaa aattgcgacg cttgcatggt
     4321 caccgaattc actgttgttg ggggcaagat tgcagcacag cgacggcagg gtgtaaaccg
     4381 gaactttccg cagccacggc cttatcatgc ttgcatccgg caactaactt tggataatgc
     4441 ccgttttcag aacactttca caagcgacta aatctttatg aaaaacgacg tcatttcacc
     4501 ggaatttgat gaaaacggcc gcccactgcg ccgtatccgt agttttgtgc gccgccaggg
     4561 gcgactgacc aaaggccagg aacatgcgct ggaaaactac tggccggtga tgggcgttga
     4621 gttcagcgaa gatatgctgg atttccccgc gctttttggc cgtgaagcgc cggtgacgct
     4681 tgagattggt tttggcatgg gggcgtcgct ggtggcaatg gctaaagatc gccctgagca
     4741 ggacttcctc ggcattgaag tgcattcacc gggcgttggt gcgtgcctgg cttctgcgca
     4801 tgaagaaggt ttaagcaacc tgcgcgtgat gtgtcacgat gcggttgaag tgctgcataa
     4861 aatgattcct gacaattcat tgcgcatggt gcagctcttt ttccctgacc cgtggcacaa
     4921 agcgcgccat aataaacgcc gtatcgtaca ggtgccgttt gccgaactgg taaaaagcaa
     4981 actgcagctg gggggtgtat tccatatggc gaccgactgg gaaccttatg cggaacatat
     5041 gcttgaagtg atgtcttcta ttgacggtta taaaaacctg tcagagagca atgattacgt
     5101 gccgcgtccg gcatcacgtc cggtgacgaa atttgaacaa cgtggtcatc gtcttggtca
     5161 cggagtatgg gacttaatgt tcgagagggt gaaataatgg caaagaaccg tagccgtcgt
     5221 ctgcgtaaaa aaatgcacat cgacgaattc caggaattag gattttcggt ggcatggcga
     5281 ttcccggaag gtacatcgga agaacagatt gataaaaccg ttgatgattt tattaacgag
     5341 gttatcgaac cgaacaaact ggcctttgac ggcagcggtt atctggcctg ggaaggtctg
     5401 atctgcatgc aggaaatcgg caaatgcacc gaagaacatc aggcgattgt gcgtaagtgg
     5461 ctggaagagc gcaaactgga tgaggtacgc accagcgaac ttttcgacgt ttggtgggac
     5521 taagaaagca tacgggcgat gacaaatgca aaactgcctg atgcgctacg cttatcaggc
     5581 ctggaaagat gcacgatcga gtaggcggga taaggtgttt acgccgcatc cggcatggaa
     5641 aacgcgtact ttgttatcaa tctggggcca gcaaatgctg gcctgatttt ttcttgaggg
     5701 aagactatga tgcgcaaaat gctgctggcg gcagcacttt cagtgacggc aatgaccgct
     5761 cacgccgact accagtgcag cgtcacgccg cgtgacgatg tgattgtcag cccgcaaacc
     5821 gtgcaggtga agggcgaaaa cggcaatctg gtgatcacgc cagacggcaa cgtgatgtat
     5881 aacggtaagc aatattccct gaatgccgcc cagcgcgagc aggcgaagga ttatcaggct
     5941 gaactacgca gcacgctgcc gtggattgat gaaggcgcga aaagccgcgt cgaaaaagcc
     6001 cgtattgcgc tggataaaat tatcgttcag gagatgggcg aaagcagcaa aatgcgcagc
     6061 cgtctgacca aacttgatgc gcagctgaaa gagcagatga accgcattat cgaaacgcgc
     6121 agcgatggcc tgacgtttca ctataaagcc attgatcagg ttcgcgccga aggccagcaa
     6181 ttagtgaatc aggcaatggg tggaatttta caggacagca ttaatgaaat gggcgcgaaa
     6241 gcggtgctga aaagcggcgg taacccatta cagaatgtgc tgggaagcct gggggggctg
     6301 caatcctcaa tccaaacaga gtggaaaaag caggaaaaag atttccagca gtttggcaaa
     6361 gatgtttgta gccgcgttgt gactctggaa gatagccgca aagccctggt cgggaattta
     6421 aaataatcct ctattttaag acggcataat acttttttat gccgtttaat tcttcgtttt
     6481 gttacctgcc tctaactttg tagatctcca aaatatattc acgttgtaaa ttgtttaacg
     6541 tcaaatttcc catacagagc taagggataa tgcgtagcgt tcacgtaact ggaggaatga
     6601 aatggagttt ttcaaaaaga cggcacttgc cgcactggtt atgggtttta gtggtgcagc
     6661 attggcatta cccaatatca ccattttagc aaccggcggg accattgccg gtggtggtga
     6721 ctccgcaacc aaatctaact acacagcggg taaagttggc gtagaaaatc tggttaatgc
     6781 ggtgccgcaa ctaaaagaca ttgcgaacgt taaaggcgag caggtagtga atatcggctc
     6841 ccaggacatg aacgataatg tctggctgac actggcgaaa aaaattaaca ccgactgcga
     6901 taaaaccgac ggcttcgtca ttacccacgg taccgacacg atggaagaaa ccgcttactt
     6961 cctcgacctg acggtgaaat gcgacaaacc ggtggtgatg gtcggcgcaa tgcgcccgtc
     7021 cacgtccatg agcgcagacg gtccattcaa cctgtataac gcggtagtga ccgcagctga
     7081 taaagcctcc gctaatcgtg gcgtgctggt ggtgatgaac gacaccgtac tggacggtcg
     7141 cgatgtcacc aaaaccaaca ccaccgacgt agcgaccttc aagtctgtta actacggtcc
     7201 tctgggatac attcacaacg gtaagattga ctaccaacgt accccggcac gtaagcacac
     7261 cagcgatacg ccattcgatg tctctaagct gaatgagctg ccgaaagtcg gcatcgttta
     7321 taactacgct aacgcatccg atcttccggc taaagcactg gtagatgcgg gctatgatgg
     7381 catcgttagc gctggtgtgg gtaatggtaa cctgtataaa tccgtgttcg acaccctggc
     7441 aaccgccgcg aaaaacggca ctgcagtagt gcgttcttcc cgcgtaccga cgggtgctac
     7501 cactcaggat gctgaagtgg atgatgcgaa atacggcttc gtcgcctctg gcacgctgaa
     7561 cccgcaaaaa gcgcgcgtcc tgctgcagct ggctctgacg caaaccaaag atccgcagca
     7621 gatccagcag atcttcaatc agtactaatc acctcgcccc ggtatcgtgc cggggctttt
     7681 tcacttcaga cttacgtcca ttgccaattt tcattaccct aatgataatc accggaataa
     7741 attattccgc gtgaggtttt tcgggtgaaa aagcaatgga ttgttggcac ggcactgctt
     7801 atgttgatga ctggtaatgc ctgggcagat ggcgaaccgc caactgaaaa tatcttaaaa
     7861 gatcaattca aaaagcagta tcacggcatt ctcaagcttg atgctatcac cttaaaaaat
     7921 cttgatgcta agggtaatca ggccacctgg tcagcggaag gcgatgtctc ttccagtgac
     7981 gatctctata cctgggtcgg tcagttggca gattacgaac tgctcgaaca gacctggacg
     8041 aaagataaac cggtaaaatt ctcggcgatg ttaaccagta aaggaacgcc cgcgtctggc
     8101 tggtcggtga acttttactc ttttcaggcg gcagccagcg atcgtgggcg ggtggtcgac
     8161 gatatcaaaa cgaataataa atatctgatc gtgaatagcg aagatttcaa tt
//
LOCUS       DAKISA010000153         7993 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.155, whole genome shotgun sequence.
ACCESSION   DAKISA010000153 DAKISA010000000
VERSION     DAKISA010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7993
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.155"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..606)
                     /locus_tag="OVW45_003934"
     CDS             complement(<1..606)
                     /locus_tag="OVW45_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase N-terminal
                     domain-containing protein"
                     /protein_id="HCV5528269.1"
                     /translation="MNTHISVSTIPHLTGWHAINWKACHARVRKLQLRIAKATRQQQW
                     RQVRELQRILTRSFSGKAVAVRRVTENTGKRTPGIDGKIWHTPKEKWEGICSLNLRGY
                     RPQPLRRIHIPKSNGKTRPLGIPTMRDRAMQALWLLALEPVSETTADHNSYGFRPMRS
                     THDAIESIFLRMSQKVSPKWILEGDIKGCFDNISHDWLQNGI"
     gene            1753..2187
                     /locus_tag="OVW45_003935"
     CDS             1753..2187
                     /locus_tag="OVW45_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HCV5528270.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFMEAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPK"
     gene            2281..2547
                     /locus_tag="OVW45_003936"
     CDS             2281..2547
                     /locus_tag="OVW45_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528271.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAQDEIN
                     PDVLAMSGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            2612..3490
                     /locus_tag="OVW45_003937"
     CDS             2612..3490
                     /locus_tag="OVW45_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HCV5528272.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGALEKALAIARQLQKMGMTPEQI
                     KQATGLSEAELKKVIH"
     gene            3552..3746
                     /locus_tag="OVW45_003938"
     CDS             3552..3746
                     /locus_tag="OVW45_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528273.1"
                     /translation="MTLAERLIQKGFDEGFKEGFKQGALEVAREIACRLRDIGWPPER
                     IQEVTGLSGEELKKLFPDEQ"
     gene            complement(3777..4028)
                     /locus_tag="OVW45_003939"
     CDS             complement(3777..4028)
                     /locus_tag="OVW45_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCV5528274.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAEDV"
     gene            complement(4087..4374)
                     /locus_tag="OVW45_003940"
     CDS             complement(4087..4374)
                     /locus_tag="OVW45_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528275.1"
                     /translation="MAPDRTTGRDPPQRREARFAKRRCPLHPGGLLPGCRERRPRRDR
                     GGATGENRRHNNGMRWSLVHHSRTGENRENSTTPTTKKSEISSLFYTYLSR"
     gene            4444..4650
                     /locus_tag="OVW45_003941"
     CDS             4444..4650
                     /locus_tag="OVW45_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCV5528276.1"
                     /translation="MYIPQNFLLKSRQKLLRSAPGQSFPAVAVMPLAAKVLKKVMVNT
                     GGNKRQVPAKRQNRGSRNSKDDGG"
     gene            4675..4962
                     /locus_tag="OVW45_003942"
     CDS             4675..4962
                     /locus_tag="OVW45_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528277.1"
                     /translation="MSTRNIHVNTASYTLLVAGKKKNTGEEWDVLEFSSLTELKKYRK
                     SHPEKMAFSYSYALSRGVDTQFRHINIAEADHFKQFLRQIKRAGLDIRAIC"
     gene            5082..5903
                     /locus_tag="OVW45_003943"
     CDS             5082..5903
                     /locus_tag="OVW45_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCV5528278.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGQEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVPGVFD
                     KVTENMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRREDKQNDLW
                     TTWQRVQENMIKGGLSWRSASGKNTRTRAITGIDGDIRINKALWVIAEQFRKWKS"
     gene            complement(6199..6846)
                     /locus_tag="OVW45_003944"
     CDS             complement(6199..6846)
                     /locus_tag="OVW45_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HCV5528279.1"
                     /translation="MILKSNQQKLVFAWGVVLLVVRTTNLLSLFVEWVKLFTDKVTRG
                     GKMKKWMLAICLMFINEICQATDCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINP
                     VTGYGSGLMQVDSQHFNELARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVTWEAV
                     GAYNAGFRKTERQNQRRLAYASDVYRIYTGIKSSKGIRIPTTKKSLPEINSVQNN"
     gene            7122..7505
                     /locus_tag="OVW45_003945"
     CDS             7122..7505
                     /locus_tag="OVW45_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome protein TraM"
                     /protein_id="HCV5528280.1"
                     /translation="MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLE
                     LGLRVYEAQMERKESAFNQAEFNKLLLECVVKTQSTVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSVEMERFFPKNDDE"
BASE COUNT         1907 a         1959 c         2060 g         2067 t
ORIGIN      
        1 gatgccattt tgcagccagt catggctgat gttatcgaaa caccctttga tatcgccctc
       61 cagtatccat ttgggcgata ctttctggct catacgcaga aaaatggact caatggcgtc
      121 atgcgtgctg cgcattggtc tgaagccata gctgttatgg tcagcagtgg tctcagatac
      181 gggttccaga gccagcagcc acagtgcctg catggcccgg tccctcattg tgggtatgcc
      241 cagtggtcgc gttttgccgt ttgattttgg gatatgtatt cgccgtagtg gctgtggtct
      301 gtaaccacgc aggttcaggc tgcatatccc ttcccatttc tccttcggtg tgtgccatat
      361 ttttccgtca ataccgggtg tccttttacc ggtgttttca gttacacgcc tgacagccac
      421 ggctttgccg gagaatgagc gggtgaggat gcgctgtagt tctctgacct gtcgccattg
      481 ttgctgtcgg gttgcctttg caatccgtaa ctgtagcttt cgcacccggg catggcatgc
      541 tttccagttt atggcgtgcc agcctgtcag gtggggtatc gtggacacac tgatgtgtgt
      601 attcatctcg ctgatatcct tttgactgct ttacagcttt tcatgcaatt aaagaccaga
      661 cggaagtctg cccgctttcg cgtcggggaa cctgccccgt atcccggtta ttacagccgg
      721 gcattcgctt tttccgtcct cctctgcccg caccgccaac agtgttcctt gcggttcacc
      781 tgcctgtgag gcagcaatac gggtttacca ggttccgctt aacatacaga aggtcagggc
      841 aggttctgtc tcttcaccgg gggtctttgc gcccatgtac tccgctgggc gaaggggagt
      901 aaccggaccc catacctttt ggttcaggcc tgtcagcatc tttggcctgt tgccgataac
      961 ggtgtttatc aacagttcac atgtgttaac ccttctgacc agccttgctc tcctgccgca
     1021 tgatgcttgc agtaaggtaa agtccctcac ggggctttac ccgggctgac gcccggctct
     1081 cgttgtcccg ggagcttcag accccgccgt tgccggcagc gcctgtcccg gtaggctaca
     1141 gctaatggga tggctggttt tatcaatgta aaacaatacg ttaagcgact tcctgtcgca
     1201 ccgcccactt cggctcccgg ctgcctgacg gcatccggtc gtcaggcccg actccgacag
     1261 gcagctgctg gcgcacccgc cagtctccgc cgaacccgct ggcgcggttt cgggctgtcg
     1321 ctacggtccg atgcgtgaaa acccggcttg cagcgaccgt tccggtcgcg ccgccctgtc
     1381 gcgccggaaa cggcgctccc tgcacacctt acggtgtgtg aggaaactgc gcctgatggc
     1441 gcagtcaacc cttccgccgc cacccctgcg gcctgtttcc cgctgtggac tttccggggg
     1501 ctggagtaac cacatcccgt ttcagcagtc caggggaaga tataccgtcc acaaaatttt
     1561 tagccataaa accggcaaaa aattttgcgt ccgcccccgg acagctgccc cgtgacgtgg
     1621 tgacggacgc cccatcggaa aggtccactg cggggagaaa ccccgaagaa gtggacggca
     1681 gaaggggctt tcgcccctcc ggggagcaac cggtcggccc ggcgacctga ttcagcagaa
     1741 ggagacgttt tcatgactgt cagcagcacg atttccgttt tttgccgcga cggggtgttc
     1801 cgtaccgttt actgccacct gcacggtgag ccgacctgga acggtcgcat cctgcatacc
     1861 cactatgcca ccggtcagca ggccgaagcc ctggttgaac acggtgatat ccgttgcctc
     1921 ggtccccgtt gcgacaaacc cgccgggcat acgcttcaga acccggtgga cggtgtgacg
     1981 gcttattacg gacgtgacag tggtttccgg atggacagtg aggcgcgtga gtaccgttct
     2041 ttcatggagg ctattgccac tgaaagcact gaagaggtgc gcttccatta tgtgttcatc
     2101 gacggctact ggaaggtgat gtaccgcacg ccggaaggat ggaagatgaa agcgctcgcg
     2161 ctggcactgc gccgctgtcc gaaatgaaaa aaaggcaggg cgataaaccc tgcctctctc
     2221 cgccggcgct tccccgccag gaagtccggc atctcaatca ctatctatgg agattatgcc
     2281 atgagaccat caattatctt cgcaaccgcc gagtatgtaa agcgtctgcg tgaagagtgc
     2341 ctgcgggaga ataaacccct gcaccgccat acccgcttca gacgtcagga gctggcacag
     2401 gatgagatta acccggacgt cctggcgatg agcggtcata tcgccagacg ctgcagtgag
     2461 cagaagcggg tacgtatccc cgccatgaaa gtcagcgaat ggggccacct gctccgcgcg
     2521 cttgaaattg aacgggtctg ccactgatta acccttgccg tctgcctgtg caggcggcag
     2581 gatacgttca gacatctcac caggaggaac cgtgagtaag aagaaaaaca ccacaacgcc
     2641 cacgccgcat gatgccgcgt tccggtcgtt cctggcgaat cccgacgtcg ccagggattt
     2701 tctggagttg catcttcctg cggagtaccg gcagttgtgc gacctgtcca ccctgaagct
     2761 ggaacccgcc acctttgttg agccggacct gcatcagtac gccagcgata tcctctggag
     2821 tgtgaaaacc accgggggtg aagacggcta tgtttatacg ctcatcgagc accagagcac
     2881 cgaaaatctg tacatgcctt ttcgcatgtt acgctacagt gtggcggcta tgcagagaca
     2941 tctggagcag cacaaaacgt tgccactggt cattccggtg ctgttctatc acggtgagcg
     3001 cagcccgtac ccgtacagca tgaactggct ggactgtttt gagaatccgg cgcttgcggc
     3061 taaaatatac acaaagccgt ttccgctggt tgatatcact gtcgttgatg acaatgaaat
     3121 catgaaccat cgccggatgg ccgcactgac gctgctgatg aagcatatcc gccatcgtga
     3181 catgatggag ctgctggaca aactcccgca ggtcatggtg gaaatttcag acgagcaggt
     3241 gcgtgttctg attcattaca tcgttaacgc aggggactct gtatcaccgg agtttatgcg
     3301 ggcgctggct gagcgcctgc cgcagcatga ggataaactg atgacaatcg cagaacgtct
     3361 tgagcagaaa ggtgcgcttg aaaaagccct ggccattgcg cgtcagcttc agaaaatggg
     3421 gatgacacct gagcagatta agcaggccac cggactttcc gaagctgaac tgaaaaaagt
     3481 cattcactga tgagagcatc acagcccggc aaaatgccgg gctttttgcc agcaggagca
     3541 gagcaaacag catgacgctt gcagaacgtc ttatacagaa aggtttcgac gagggtttta
     3601 aagaaggttt taaacaaggg gctcttgaag tggcgcggga aatagcctgc cggcttcggg
     3661 atataggctg gccgccggaa cggattcagg aggtcacggg actttccggt gaagaactga
     3721 aaaagctgtt tcctgatgag cagtagcctg gcattcagcc aggctgtgaa tcacgctcac
     3781 acatcctctg ccgtttcccc cagttcattt tccagtcgcc tgatgatatc tgccttacgc
     3841 atcccctgca tgtgaggcag caggtcacgg actgaaagtg cctgggtggt cctccagacg
     3901 gtgacgtcca cctgttccgc attttcccag gcgtgccatg ttctctgcgt cacgccaaat
     3961 tctctggccg ctttttcgcg tgaccagagc atgctttttc gccagattcg cagttcccag
     4021 ccggtcataa aacacctttg aaaaagtgaa aaaacttcac tttattttat catcacaatt
     4081 gccaaatcag cgcgataaat aagtgtaaaa aagtgaagaa atttcacttt ttttagttgt
     4141 aggtgtcgtg ctgttttctc tgttttcacc tgtgcgggag tggtgtacca gtgaccaccg
     4201 catgccgttg ttgtgccgtc ggttttctcc ggtcgcgccg cctctgtcgc gccgtggcct
     4261 gcgctcccgg catcccggca aaaggccgcc gggatgtaag gggcaacgcc gtttcgcaaa
     4321 ccgcgcttca cgtcgttgcg gcgggtccct gcccgttgtt ctgtccggtg ccattcacct
     4381 gtttaagccc ctgtcgccgg gggctgatac agccacaacc gtctccgcca gtccaggggt
     4441 gaaatgtaca tcccgcaaaa ctttttgctc aaaagcaggc aaaaactttt gcggtctgcc
     4501 cccggacagt cgtttccggc cgtggcagtg atgccccttg cggcgaaggt tctcaaaaag
     4561 gtgatggtga ataccggagg gaacaaaagg caggtccccg caaaacgtca aaaccgcggc
     4621 tcccgaaact caaaagatga tgggggctga aaaccagaga gaggagaaag caggatgagt
     4681 acccgaaata tccacgttaa caccgcgtcg tacacgcttc ttgttgccgg gaagaaaaaa
     4741 aacaccgggg aagagtggga cgtcctggaa ttcagcagcc tgactgagct gaaaaaatat
     4801 cgcaaaagcc acccggaaaa gatggccttc agctatagct acgcgctcag ccggggtgtg
     4861 gatacgcagt tccgccatat caacattgcg gaagccgatc acttcaaaca gttcctgcgt
     4921 cagattaagc gtgccggcct cgatatccgg gctatctgct gaccggatgt gatgaatatc
     4981 cccccctgat tgttgcaatc ttgttaatgg agatcgggag ggatgatatt aagcggaaaa
     5041 attttttatt aaatttattc tttcataaag gagtagctgt tatgcgatta gcttcccgtt
     5101 ttggtcggta taattccatc cgccgtgaac gtcctttaac ggatgatgaa ttaatgcagt
     5161 tcgtgccttc ggtattttcc ggtgataaac atgagtcccg gagtgaacgt tatacgtata
     5221 ttccaacaat caatatcatc aataagttac gtgatgaagg tttccagcca ttctttgcct
     5281 gtcagagtcg ggttcgtgat ttgggacgtc gcgaatacag taaacatatg ttacgtcttc
     5341 gcagggaagg gcatattaac gggcaggaag ttcctgaaat tatcctgctt aattcacacg
     5401 atggttcatc cagttatcag atgatccccg gaatttttcg ttttgtctgc acaaatggcc
     5461 tggtgtgcgg aaataatttt ggcgaaattc gcgttccaca taaaggtgat attgtcgggc
     5521 aggttatcga gggagcgtat gaagtgcccg gtgtctttga taaggtcact gaaaatatgg
     5581 aggcgatgaa agaaattcat cttaacagtg acgagcaaca tttatttggc agagctgcac
     5641 tgatggtcag gtatgaagat gaaaataaaa cgccagtgac gcctgaacaa ataattactc
     5701 cccgtcgtcg ggaggataaa cagaacgatc tctggacaac ctggcagcgg gttcaggaga
     5761 atatgataaa aggtggatta tcgtggcgaa gtgcctccgg gaaaaatacc aggacaagag
     5821 ccattacagg tattgatggt gatataagaa tcaacaaggc gttatgggtg attgccgaac
     5881 agttcagaaa gtggaagtca tgataatata agtaaagccc cgaaaaattt tcggggcttt
     5941 acttatatta tcattcttcg gcatcgctgt gtcgtctgat atacctcaat ttcatcaact
     6001 ggctttccgg ttgtctgtaa atattgtgcc cgcgaaaaat ggtctggcag gaggctgcgc
     6061 agttaccggg tatatataag atgggttccc gcttcgcggg gcttcggcca gtcagtattg
     6121 tcactcatat gatatttttg tgtgtggcct tccatgccgc ttttgcggca taaccggtat
     6181 ctgacaatgt ctgtaagatt aattgttctg cacgctgtta atttccggaa gtgatttctt
     6241 cgtggtcgga atccggatac ctttactgct ttttatcccg gtataaatcc ggtaaacatc
     6301 tgatgcgtag gcaagacgtc tctggttctg gcgttcggtc ttcctgaatc cggcattgta
     6361 tgcaccaacg gcctcccagg tgacgcccca ttttttaaat gctattgcca gataataagc
     6421 accggtataa atgttcatgc agggatctgt tgtcagatgt tccggcttaa ttccatagcg
     6481 ggccagttcg ttaaaatgct gggaatctac ctgcatcagt ccgctgccat atcccgttac
     6541 cggattaata ccgatggcat taacccggta acgggattct ttccatgata ttgctctcag
     6601 taaatcagga tctattttgt aatcccggcc tgcaagatca aagcaatcag tggcctggca
     6661 gatctcattt ataaacatca ggcagatggc taacatccat tttttcattt ttccacctct
     6721 ggtgacttta tccgtaaata atttaaccca ctccacaaaa aggctcaaca ggttggtggt
     6781 tctcaccacc aaaagcacca caccccacgc aaaaacaagt ttttgctgat ttgattttaa
     6841 tatcatatgc ttatattcat gaatttatat tgtttaaatc agatttattt aaaacagcgg
     6901 tgcaggcgcg gctatggcac cgtgtctgca ccgctttgta ggggtggtac tgactatttt
     6961 tataaaaaac attgttttat attaggggcg ctgctagcgg cgcggtgtgt ttttttatag
     7021 gataccgcca ggggcgctgc tagcggcgcg ttcctgtttc cattgtgaat tttagtgttt
     7081 cggaattaac tttattttat gtttaaaaaa ggtaatttct aatggctaag gtgaacctgt
     7141 atatcagcaa tgatgcttat gaaaaaataa atgcgattat tgaaaaacgt cgacaggagg
     7201 gggcgagaga aaaagatgtc agtttttcag caacagcttc aatgcttctt gagttagggc
     7261 ttcgtgtata tgaagctcag atggagcgta aagaatcagc atttaatcag gcagagttta
     7321 ataaacttct tcttgaatgt gttgtaaaaa cgcagtcaac ggtggcaaag attttaggta
     7381 ttgagtctct tagccctcat gtttctggaa acccgaagtt tgaatatgcc aatatggttg
     7441 aagatatcag agagaaggtg tcggttgaga tggaaaggtt ctttccaaaa aatgatgatg
     7501 aataaaggga aataaatttt gactttgttc aaatatcaga gttttttttg gttcaaactg
     7561 atgacaataa tcagaataat tagtatattg attacgtggt taatgccacg ttaaaaattg
     7621 aaactgaaaa tcgccgatgc agggagtggc tgctccctgc atcgactgtc catagaatcc
     7681 ttagaggagg ttcctatgtg tgcgatggat cgtagagaaa gagcactcat cagtcaattg
     7741 catttccttc atagtcttgg agaggctatt tcgtttttaa aatatcctgt atgtgtccgc
     7801 ttcgaggatg gttcttttat taaggaaaat aactgctttg aaaaacttat cagatcgtca
     7861 tttaattcat gtgatgaatg gtttgatagt ttaacgttgg aatgtaaatt acagttatcc
     7921 agagcagaga ttgaatcatg ctcttctatt tatggtgtaa attataataa tgatatatta
     7981 cttaataata cgt
//
LOCUS       DAKISA010000154         7807 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.156, whole genome shotgun sequence.
ACCESSION   DAKISA010000154 DAKISA010000000
VERSION     DAKISA010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7807
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.156"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..220
                     /locus_tag="OVW45_003946"
     CDS             <1..220
                     /locus_tag="OVW45_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="acts in anaerobic beta-oxidation of fatty acids;
                     possible ligase/synthase involved in anaerobic growth on
                     fatty acids; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclohexanecarboxylate-CoA ligase"
                     /protein_id="HCV5528281.1"
                     /translation="CAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKYPEHIVVIEKLPR
                     TASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(277..2655)
                     /gene="ppsA"
                     /locus_tag="OVW45_003947"
     CDS             complement(277..2655)
                     /gene="ppsA"
                     /locus_tag="OVW45_003947"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCV5528282.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHEISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVEMMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            2988..3821
                     /gene="ppsR"
                     /locus_tag="OVW45_003948"
     CDS             2988..3821
                     /gene="ppsR"
                     /locus_tag="OVW45_003948"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCV5528283.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            3978..5024
                     /gene="aroH"
                     /locus_tag="OVW45_003949"
     CDS             3978..5024
                     /gene="aroH"
                     /locus_tag="OVW45_003949"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCV5528284.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGAATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYAPRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLLEGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            5156..5347
                     /gene="hemP"
                     /locus_tag="OVW45_003950"
     CDS             5156..5347
                     /gene="hemP"
                     /locus_tag="OVW45_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCV5528285.1"
                     /translation="MRYTDSRKLTPETDANHKAASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(5351..6787)
                     /locus_tag="OVW45_003951"
     CDS             complement(5351..6787)
                     /locus_tag="OVW45_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCV5528286.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FITEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSTASPLRDEFIDRAAFDGW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHGAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(6850..7563)
                     /gene="ydiV"
                     /locus_tag="OVW45_003952"
     CDS             complement(6850..7563)
                     /gene="ydiV"
                     /locus_tag="OVW45_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCV5528287.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
BASE COUNT         1989 a         2058 c         1853 g         1907 t
ORIGIN      
        1 atgcgcttat gtcgtgctga aagcgccgca tcattcatta tcgctggaag aggtggtggc
       61 tttttttagc cgtaaacggg tcgcaaaata taaatatcct gaacatatcg tggtaatcga
      121 aaaactaccg cgtactgcct caggtaaaat acaaaagttt ttgttaagaa aagatattat
      181 gcggcggtta actcaggatg tctgtgaaga gattgaataa gtttcatctt cggggatcac
      241 ataaccccgg cgactgaacg ccgccgggga tttattttat ttcttcagtt cagccaagct
      301 taaccaggtt tgcaccacgg tgtccgggtt cagagacagg ctatcgatcc cctcttccat
      361 caaccatgcg gcaaagtctt cgtggtcgga cggaccctga ccgcaaatcc cgacatattt
      421 gccctgtttc ttcgcggcac ggatagccat cgacagcagt gctttcaccg catcgttgcg
      481 ctcatcgaac aattcagaca ccacgccgga gtcacggtcc agaccgagcg ccagctgcgt
      541 catatcgttt gagccaattg agaagccgtc gaaatattcg aggaactgct cggccagcaa
      601 ggcgttggac gggatttcac acatcatgat gattttcagc ccgttctcac cacgcttcag
      661 cccctgacgc gccagttcct caaccaccgc tttcgcctga tctacggtac gcacgaacgg
      721 gatcatgatc tcaacgttgg tcagccccat gtcgttgcgc acacgtttca ctgcttcgca
      781 ctccagcgcg aagcagtcgc ggaagctgtc ggaaacatag cgtcccgcgc cacggaagcc
      841 gagcatcggg ttctcttcat ctggctcgta acgctcacca ccgaccaggt tggcatattc
      901 gttcgattta aaatcagaga gacggacaat gacgcgcttc ggataaaacg cggcacccag
      961 cgtcgcgatc ccttcagtca gacgaccaac gtaaaattca cgcggagaat caaaaccttt
     1021 catcatctcg cggatttcat tttgcaactg cggttcctga tcgtcaaact caagcagtgc
     1081 gcgtgggtgg acgccaatca tacggttgat gataaattcc agacgcgcaa ggcccacgcc
     1141 ttcgttcggc aggcaggcga agtcgaaagc acggtccggg ttaccgacgt tcatcatcac
     1201 tttcaacggc agatccggca tcatttctac gctggagctt ttcacgctaa attccagcaa
     1261 ctccgcatag acgtaaccgg tatcaccttc ggcacaagaa acagtgacgt tctcaccgtc
     1321 tttcatccgt tctgttgcat caccacagcc cactaccgcc ggaatgccca gttcacgagc
     1381 gatgatcgcc gcgtgacagg tacgaccgcc acggttggtg acgatggcag atgctttctt
     1441 catgatcggt tcccagtccg ggtcggtcat gtcagtgacc agcacgtcac caggttcgat
     1501 gcggttcatt tcgctgatct catggatgac tttcaccgga cccgcgccga tgcgatgacc
     1561 gatagcacgg ccttcggcga taatcttacc ctgtgaatgc agcgtataac gctccatgac
     1621 ctgaccgcgt gagcgcacgg tttccggacg cgcctgcaca atgaacagtt tgccagtgtg
     1681 gccatctttc gcccactcaa tatccatcgg gcgaccgtag tgtttctcaa tttgtacggc
     1741 ctgttttgcc agttcctgca cttcttcgtt ggtcagcgag aagatgtcac gctgttcctg
     1801 cggtacgtct tcgattttaa cctgcttgcc gtgctcctgg gtcggcgcgt aaaccatgcg
     1861 gatttttttc gaccccatgg tgcgacgcac gatagccggg cgattcgccg ccagtgtcgg
     1921 tttatggacg taaaactcat ccgggttaac cgcaccctgc acgaccattt ccccaaggcc
     1981 ccatgcggaa gtgataaaca ccacctggtc aaagccggat tcggtatcaa tggagaacat
     2041 cacgccagaa gatgcgaggt cggagcgcac catccgctga acaccggcag agagcgccac
     2101 gccacggtga tcgtaaccct ggtgcacacg ataagagatg gcgcgatcgt taaacagaga
     2161 agcaaataca tgcttcactg ccacgagaac ggcgtcaaaa ccctgaacgt tgaggaaggt
     2221 ttcctgctga ccggcaaaag aagcgtccgg catatcttct gcggtggcgg aggagcgcac
     2281 cgcaaaagag gcgttttcgt catcggcgga aagctgtgca taggcttcgc ggatggcgtt
     2341 ttccagctca ggctggaagg gagtgtcgat aatccactgg cggatttgcg cgcccgcttt
     2401 cgcaagctga gtaacatcgt caatatccgt tttatccagc agttcataaa tgcgctggtt
     2461 tacgccgctt tggtccagaa actggttaaa cgcgtcggcg gttgtggcga aaccattcgg
     2521 aacggaaaca cccattccgg aaaggttagt aatcatttca cccagggagg catttttgcc
     2581 cccaaccctg tctacatcat tcatgccgag ttggttatac caaagcacca gcggtgacga
     2641 gccattgttg gacatcgaac aatccttttg tgataaatga acggtttgag aaacacattt
     2701 ctgcgcattt atctttgcat atttaaccgg atgaaaaaaa cggtgaatcg ttcaagcaaa
     2761 tatatttttt tactttttaa gactgatccc agcgttgcgc aaatctgctc tcttcgacaa
     2821 tttccacata aaccatcggt ataaacgaaa ccataaaaat gaaatgctgt tttcataaaa
     2881 aataaaattg aaggttcatt ttataaacca gacataacgt cctacgcttc tgtgcgtttt
     2941 taatttatgc tttcatagaa ttatgtctgc atcacgggaa gaacaaaatg gataatgctg
     3001 ttgatcgcca cgttttttat atttctgatg gtacggcaat aactgcggag gtattaggac
     3061 acgcagtaat gtcacaattt cccgtcacta tcagcagcat tacgctgccg tttgtcgaaa
     3121 atgagagccg tgcacgggca gtgaaggatc agattgacgc aatttatcac cagacaggtg
     3181 tgcgcccgct ggtcttctac tccatcgtgt tgccggagat tcgcgccatc atcttgcaaa
     3241 gtgaaggctt ttgccaggat atcgttcagg cgctggttgc cccgctacaa caagagatga
     3301 aactggatcc aacgccgatt gctcatcgta cccatggcct taaccctaat aatctcaata
     3361 aatatgatgc gcgcattgcg gcgattgatt acaccctcgc ccacgatgac ggcatttcgt
     3421 tgcgcaatct ggatcaggct caggtgatcc tgctcggtgt ttctcgctgt ggtaaaaccc
     3481 ccaccagtct ttatctggca atgcaatttg gtatccgcgc ggcaaactac ccctttattg
     3541 ccgacgatat ggataatctg gtgctacccg cgtcgctcaa accgcttcag cataaattgt
     3601 tcggcctgac tatcgacccg gaacgtctgg cggcgattcg cgaggaacgt cgggagaaca
     3661 gtcgctatgc ctcgcttcgt cagtgcagga tggaagtcgc ggaagtggaa gcgttgtacc
     3721 gtaaaaatca gatcccgtgg attaacagta ccaattattc ggtagaagag attgccacca
     3781 agatcctcga tatcatgggc cttagtcgcc gaatgtacta gagaactagt gcattagctt
     3841 atttttttgt tatgatgcta accacccggc gaggtgtgac acacctcgca cttgaaatca
     3901 gcagcgattg gtttatcgtg atgcgcatca cttcccggca gtcctgccgt agaagcaaca
     3961 aatttctgag acttgtaatg aacagaactg acgaactccg tactgcgcgt attgagagcc
     4021 tggtaacgcc cgccgaactc gcgctacggt atcccgtaac gcctggcgcc gccacccatg
     4081 tcaccgactc ccgccgcaga attgaaaaaa tactcaatgg tgaagataag cgactgttgg
     4141 tcattattgg cccctgctcg atccacgatc tcaccgctgc aatggagtac gccccccgtc
     4201 tgcagtcgct gcgcaaccag taccagtcac ggctggaaat cgtaatgcgc acctattttg
     4261 aaaaaccacg aactgttgtc ggctggaaag gactaatctc cgatccagat ttaaacggca
     4321 gctatcgggt aaatcacggt ctggagctgg cgcgcaaatt acttttacag gtaaatgagc
     4381 tgggcgtccc aaccgcgact gagttcctcg atatggtgac cggtcagttt attgctgatt
     4441 taatcagttg gggcgcgatt ggcgcacgta ctaccgaaag tcagatccac cgtgaaatgg
     4501 cttcggcact ctcctgtccg gtaggtttta aaaatggtac cgatggcaat acgcggattg
     4561 ctgtggatgc tatccgcgca gcccgcgcca gccatatgtt cctctcgcca gacaaaaatg
     4621 gtcagatgac catctatcag accagcggca acccgtatgg ccacattatt atgcgtggcg
     4681 gcaaaaaacc gaattatcat gccgatgata tcgccgcagc ctgcgatacg ctgcacgagt
     4741 ttgatttacc tgaacatctg gtggtggatt tcagccacgg taactgccag aagcagcacc
     4801 gtcgccagtt agaagtttgt gaggatattt gtcagcaaat ccgcaatggc tctacggcga
     4861 ttgctggaat tatggcggaa agtttcctgc tcgaaggaac gcaaaaaatc gtcggcagtc
     4921 agccgctcac ttacggtcaa tccattaccg acccgtgtct gggctgggaa gataccgaac
     4981 gcctggtcga aaaactcgcc tctgcggtag atacccgctt ctgaatgcgt gcccattcct
     5041 gacggaatgg gcatttctgc gcaacttgtt gtcttctcaa caaattactg cttgctctgg
     5101 tctgccataa tattgataat aagaatcatt gttatatcaa ttattattaa tctttatgcg
     5161 ttatacggat agcagaaaac tcacgcctga aacggacgcc aatcacaagg ccgcttcccc
     5221 gcagcccatc cggcgaatta ccagccagac actgttaggt ccggatggca aactgattat
     5281 cgatcatgac gggcaagaat atctgctccg taaaactcag gctggcaagc tgctgttaac
     5341 taagtagcct ttaactcgag cagctgactt ccagccgttt accccagtca ggtggacgac
     5401 tgacatagtc atcatctcta tcgctgaaag gatttcgcaa cgccccatgc aggcggtgca
     5461 attccgtcat atcacccttt tctgccgctt caatcgcccg ttgcgccaac cagttgcgca
     5521 ataccagagc ggggttaacg ctttgcatca actgctgacg ctcactatcg ctaacctcgt
     5581 cttgttgcaa acgcccccga taacgggcaa accagccatc aaatgccgca cgatcaataa
     5641 actcatcacg tagcggtgac gccgtgctgt gctgctcggt cagactcagc atgcggaatg
     5701 tgcgggtata atcgctgcgc tctcgcgcca tcagactgaa taattcattc agtagcgcgt
     5761 tatcctcttt ttgctccgtg atgaagccca gtttctgccg catccgttgt ccataatgcg
     5821 tcaacaaaac ctgctgatag ctgtccaggg cctcattcag ggcatctacg gcaacaaatg
     5881 gtgacaatgt ctgcgccaga cgctgtaaat tccacaacgc gacagcaggt tgattatcaa
     5941 aactataacg cccttgatga tccgagtgat tacaaataaa accgggttcg taatcatcaa
     6001 gaaaaccaaa cggcccgtaa tcaagcgtca gtcccagcag cgacatgttg tcggtattca
     6061 tcaccccatg tgcaaagccg accgtctgcc attgggcaat taacgacgcg gtacgtgcga
     6121 caacatcgct aaaccagaga cggtatttgt cctcatcatc ttcaagatgt gaccagtaat
     6181 gacgaatagc aaagtcagct aactgacgaa ccttttccga ctcgcggcgg tagtaaaaat
     6241 gttcgaaatg accaaagcgc agatgacttg gtgccacacg catcagcatc gcgcctggct
     6301 ccgccgtttc ccgatacact ggcgaatcgc tggtgacgat acttaacgcg cgggtcgtcg
     6361 gaatgcccag ataatgcatc gcctcactgg cgagactttc tcgtatcgtc gaacgtaaaa
     6421 ccgcccgtcc atcacccatt cgcgaataag gcgtcaggcc agcacctttc agatgccagt
     6481 ccattgtagt gccatcagca agcagttgtt cgccgagtaa aatgccgcgc ccatcaccca
     6541 gttggcccgc ccagacgccg aactgatgac cactgtaaac ctgcgccagt ggtgacatac
     6601 caggcagtag ggcttcgccg ccccagacac ctgcgccatt tttaaacagc gacgatggaa
     6661 tactcagcgt gttagccagt tcggtattat gccaaatcag ccgggcatta tttaaaggcg
     6721 taggggaaag tgctgtatag gtttctggca attcatcgcg ccagcgggta acaaaagaca
     6781 gggtcataga tcctcctgtt tgatagtgta gacggttact ctcgtcttaa acaccagcaa
     6841 acagaagggt tatcgctgaa ccaacgtcgt tatctgattt atgggaacgg caggccacag
     6901 gcatccctgg agagcatgga aatcgaaggg ggtaatctgt gccaatattt ctgccgtatc
     6961 aatgcctccc gcaatgatgc aattgcaaca aggggaaatt tgcgcctgga tcgcacgaat
     7021 aaacggttcg aaggagcggt gcgtgatttg ttgctgaata aaacttttat ccagcatgac
     7081 gcgggtgaaa agaccatcaa aaaccgcttt cattgtgcta ttgcctgctc ccaaattacc
     7141 taacacaagg ggatacactt gcgataagga taaaagaccg cgattatctt ttccttcatt
     7201 gagatgcggg taattctcat taatcaacag ttcaataaac ggatatttta atagttcgcc
     7261 tgcgtaatta tccctctcta ataataatgt agccacttgt ggcgttaaat taagccaggc
     7321 aaagagttta tgttgaataa aaaaatgttg gcatgatttt agtaattcca actgctctga
     7381 aaataattgc caatgttgct cctcggtaag ttgcgctata acccgactcg tcgggatacg
     7441 caccgtgcca tcttcactgg agaaatgggt aattaactca acgccgacaa ggacttgctg
     7501 gttatctctt atcggcagga aatagcaatc agaatgataa agattctcca aaaaaatctt
     7561 catggtaacc gtccctctcg aaggatgttt ttcagtatcc ggctaaaagg gatgaggcca
     7621 taagacataa caattacaga aggagtaact ttcatttgtt ccatgttaac cactttttca
     7681 ggggtccttt ttttagatta tcctgattat aaacgaataa tccatttacg gaatttttgt
     7741 ctgcaaaata ctactgtatt caggagtaaa tggacggcga tgtatcatcg ccgtccattt
     7801 ttcgaca
//
LOCUS       DAKISA010000155         7794 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.157, whole genome shotgun sequence.
ACCESSION   DAKISA010000155 DAKISA010000000
VERSION     DAKISA010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7794
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.157"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(102..416)
                     /locus_tag="OVW45_003953"
     CDS             complement(102..416)
                     /locus_tag="OVW45_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HCV5528288.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDELLPETRPCSEARYTIKINSSDNSIT
                     GC"
     gene            complement(421..636)
                     /locus_tag="OVW45_003954"
     CDS             complement(421..636)
                     /locus_tag="OVW45_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCV5528289.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            complement(787..2640)
                     /locus_tag="OVW45_003955"
     CDS             complement(787..2640)
                     /locus_tag="OVW45_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCV5528290.1"
                     /translation="MAFKHYDVVRAASPSDLAKRLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPHPRIKQLARRN
                     TVTPGGEVCVFNDIIPADHCLHDVQDMSTINHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGTEGTFSESTGASQDSARWGVGKPLYQDLLFRTKAAL
                     QKNPKNVLLAICWMQGEFDMTNASYAQQPAAFLAMVQQFRADLAGLAAQCHGGSPASV
                     PWICGDTTYAWKQEHGTQYEVVYGAYKGKESQQIYFVPFMTDGSGVNTPTNNPSEDPD
                     IAGSGYYGSASRTNKNWVSSNRPTHFSSWARRGIIPDRMATAILNVAGRTLAFISGKA
                     PEIKPSPGGDTPSGPSEDASIRTISLLPTAGDAAAQGWSIKNGGVQLSDGVFKITKQS
                     NKAWSLTRPVDDAVSLLTRGGRLSCKFRLSGALTNNQFGLGIYLYTDVALPDVVAMTG
                     TGNPFLMSFFTQTTDGKLNLMHHKKAGNTKLGEFGNYSNDWLTLELVFTAGSATVTPK
                     LNGVAGPAFQVIKDSLTLGLNALTLTDITKNAAYGVEIESLVLEINAPASS"
     gene            complement(3616..3692)
                     /locus_tag="OVW45_003956"
     tRNA            complement(3616..3692)
                     /locus_tag="OVW45_003956"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3656..3658),aa:Arg,seq:tct)
     gene            complement(3706..3782)
                     /locus_tag="OVW45_003957"
     tRNA            complement(3706..3782)
                     /locus_tag="OVW45_003957"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3746..3748),aa:Arg,seq:tcg)
     gene            complement(3790..3865)
                     /locus_tag="OVW45_003958"
     tRNA            complement(3790..3865)
                     /locus_tag="OVW45_003958"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3830..3832),aa:Ile,seq:cat)
     gene            complement(3906..4964)
                     /locus_tag="OVW45_003959"
     CDS             complement(3906..4964)
                     /locus_tag="OVW45_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HCV5528291.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYAAKGSALKQHVMA
                     PLISYFRDARAALGITAKQIADATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHRRGELEKLHHQLVDTYTSLNRQYAELLSEYKHLRRYFGVTVQVPYTDVWTHKPV
                     QFYPGKHPCEKPAEMLQQIISASSRPGDLIADFFMGLGSTVKAALALGRRAIGVELET
                     ERFEQTVREVQDLVSQNG"
     gene            complement(5072..5312)
                     /locus_tag="OVW45_003960"
                     /pseudo
     CDS             complement(5072..5312)
                     /locus_tag="OVW45_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5537..6358)
                     /locus_tag="OVW45_003961"
     CDS             complement(5537..6358)
                     /locus_tag="OVW45_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCV5528292.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGVSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(6355..6735)
                     /locus_tag="OVW45_003962"
     CDS             complement(6355..6735)
                     /locus_tag="OVW45_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCV5528293.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     AQPVSGGRLGVKIYPIMLEGQVKK"
     gene            complement(6736..7722)
                     /locus_tag="OVW45_003963"
     CDS             complement(6736..7722)
                     /locus_tag="OVW45_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCV5528294.1"
                     /translation="MSAFHNGRILVEPEPKSMRNLPSGVVPAARQPLVEDKTLLPFFS
                     NARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLR
                     DQTSESLEQLAHQNLSAWMIDVIGHAISGTQERELSLAELSWWAVRNQVADALPEAVL
                     RRSLGLRAEKIRSVYSESDIIPGEQTATSILKQRTKNIALLPHVHQQQNPPQEKTVVS
                     IAVDPESPAQYLQRQKSQREEMPVYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTK
                     ADDLFVIPLCRKCRNELHAGVKDFEEKHGSQLLLLIRFLMHARNSGVLKWKA"
BASE COUNT         1831 a         2207 c         1811 g         1945 t
ORIGIN      
        1 gcgccagaac agcagctgac aggccgtatt tgatttttgc gctcatggat atttatcagg
       61 atgctaccaa tgaaagatac tggaaagcca actgcaaaaa gctaacaacc cgtaatcgag
      121 ttatcagaac tgttaatttt tatggtatac cgcgcctctg aacaggggcg cgtttctggc
      181 aacagctcgt ccccttcaca taacccggca gcaacatcca ggaagacctg tctgatgctc
      241 cttctggctg ctgcctcata aaactccagc gcggcacctt caacacggtc cagcgagatg
      301 tccaggtcaa aaatttcacc gtcaaagcgt tttttgtccc gtaacgctaa agttaccgta
      361 actttattct caaaattgcg gatccctttc acaatcagtt catagttttg agtcattgaa
      421 ttactctccc cgtgcagcct tacgacggtc ctctctgatt ttgaaataca ggttagtcag
      481 atatgtcagc agcccaaaca gcagactccc cagcacgcct attgccgccc actgagacgg
      541 ggaaaccctg tccagcaact gcaggaacca gtagcccgtt cccaccgctg acgtggtgta
      601 tgacacacct gttgtgattt tttccatctg gtacataccc cgtctcccgt tatccggaag
      661 ctgacaacaa taaaaaaagc caccagttaa gtactgatgg ctctgataac tcatgcaggc
      721 atctcagacg acccactgac actaccggtg agtttaacga taccttccat ttggctggct
      781 cactttttat gatgatgccg gtgcatttat ctccagcacc agactttcta tctcaacgcc
      841 atacgctgca tttttggtaa tatccgtcag cgtcagcgca ttcagcccca gtgtcagact
      901 gtcttttatg acctggaatg ccgggccagc cactccattc agtttcggag taaccgtggc
      961 actgccggcg gtgaacacca gctccagcgt cagccagtcg ttactgtaat tcccgaactc
     1021 gcccaacttt gtgtttcctg ctttcttgtg atgcatcaga ttcagtttgc cgtctgtggt
     1081 ctgggtgaag aacgacatca ggaacgggtt accagtcccg gtcatcgcca cgacgtcagg
     1141 taacgctaca tcggtataca gataaattcc cagaccgaac tggttgttgg tcagtgcgcc
     1201 tgacagtcga aacttacagc tcagtctgcc accccgtgtc agcagggaga ctgcgtcatc
     1261 caccgggcgc gtcagggacc aggctttatt gctctgcttg gtgatcttaa atacaccatc
     1321 tgacaactga actccgccat tcttaatgct ccagccctgc gcagcagcgt ctccggctgt
     1381 cggcaacagg gagattgtgc gtatggatgc atcttcagac ggccccgatg gcgtgtcgcc
     1441 gccgggcgag ggtttgattt ccggtgcctt accactaatg aaggctaagg tgcgaccggc
     1501 tacgttcaga atagcagttg ccatacgatc gggaataatg ccacgacgcg cccatgagct
     1561 gaaatgcgtc gggcgatttg atgataccca gtttttgttc gttcgggatg ccgaaccgta
     1621 ataaccagac ccggcaatat caggatcttc tgacgggttg tttgtcggtg tattaactcc
     1681 gctaccatcg gtcataaagg gaacaaaata aatctgctgg gattctttac ctttatatgc
     1741 accatatacc acttcatatt gcgtaccgtg ttcttgtttc cacgcgtatg tcgtgtcgcc
     1801 acaaatccag gggactgatg ccggacttcc accgtgacac tgcgccgcca gcccggcaag
     1861 gtcagcacgg aactgctgta ccattgcaag aaatgctgct ggctgctggg cgtaactggc
     1921 attcgtcata tcgaattccc cctgcatcca gcatatcgcc agcaaaacgt ttttcgggtt
     1981 tttctgcaat gctgccttcg tgcggaaaag cagatcctga tataacggct tacccactcc
     2041 ccagcgagcc gaatcctgac tggcccccgt ggactcgctg aatgtcccct ccgtgccctg
     2101 ggtgaatgcc gaaccaccac gacagcatgg taccagcagg atccccgcat tattagggat
     2161 atacggaagc agttttttgg caatatgtaa gccctgtccg acacagccgt actgcccttt
     2221 gctcaggtca gcccggggat ggttaatcgt actcatatcc tgaacatcat gcagacaatg
     2281 gtcagcagga atgatgtcgt taaatacgca tacttcacca ccgggagtca ctgtgttacg
     2341 acgggccagt tgcttaatgc gcggatgggg cgcatcgtat gaatccggaa gcggaagacc
     2401 ttcaccgtaa gccatggcat tggattgccc ggccagtacg atgacgtagt accactccgg
     2461 ctcagttgca ccactgacga ccacatcacc ttctgctgca atcgcctgca tcagggtata
     2521 aggggttatg gccaccggac taccaaacgg ctgccagccc tccttcagtt tttgtgtcag
     2581 tcgtttcgca aggtctgacg gcgatgccgc cctgaccacg tcatagtgtt taaatgccat
     2641 gaatcctccc ggccgggata atattgtgag taaaataagg agcgggctga agtccggaag
     2701 ttacaggaca atggcagaag agagacgaca gcccgcaatt cgaaaaagac cgcgcagttg
     2761 cgcagagtga ttactatggg gtattattcg ccagctgaaa tattacttca cgttttattg
     2821 tttattcctt gccgcccgcg tctcccagcg cgggcttttt ttgcccacaa gaaaacccct
     2881 ccggagaggg gctaaagccg cgtatctgta tcatcatgca catggtgccg ggtgcctccc
     2941 ggtgagttca gcccggtgcc actaaacccg cgtcattctc gttttgataa tcagagatta
     3001 taccgtcacc agtcgcccct ccgctcaggg ggattcacca tgcgaaattt ttttaacaaa
     3061 tgcccagtct gacaggcaac tgtcaactta ctgaattgtg agcaacatag catttaacgg
     3121 ggaacctgtt ttctgcagta aaaaggccca ccggagcgga tgggcctgga aggatagcgg
     3181 tcatgtgatg ccggtttccc ggtaactcag caccggtatc tgagtcaacg ttttctctac
     3241 tgggtcattt ccgatacgcc ctgcctgctg acaggctttc atcacatctg aaaatatagc
     3301 accctgactg atactgtagt acctaaggtt ccagaaactg tgatgtatcc ggcacagaaa
     3361 agcccctccg gagagggctg gagagtggcg ctatgtgcca ttgcatgatg ccgggtgcct
     3421 cccggtgaat tcagtaccag cacctgaatc cgcgattatc ccatatacct actcgctgat
     3481 tgcccctccg cacaggggga ttcaccatgc cagtttcttt taacaaactc cccgcaaaac
     3541 agacaactgt caaccgtctg aattgtgaga catttaaaaa aaaggcccgc aaaagcgagc
     3601 cagggaaaat aagtgtggcg cgttgtactg gattcgaacc agtgaccgat tgcttagaag
     3661 gcaattgctc tgtccggctg agctaacaac gcatgatgca gataatggac cgccatcggg
     3721 gacttgaacc ccgcgcagcc agcttcgaag gctggcgctc tttcctgatg agctaatggc
     3781 ggtatgtgat ggtggccctt gctggatttg aaccagcgac ctggcgatta tgagtcgctc
     3841 gctctcacca ctgagctaaa gggccgggag ccgcataata acgacgcgta attaattctt
     3901 caatatcatc cgttctggct gactaaatcc tgtacttccc gaaccgtctg ctcaaaacgt
     3961 tcagtctcca gctcaacgcc aattgcacga cgcccgagcg ccagtgccgc tttcactgtc
     4021 gaacccaacc ccatgaaaaa atctgcaatc aggtcacccg gacgactgct agcgctgatt
     4081 atctgctgca gcatttctgc cggtttttcg cacggatgtt tcccgggata gaactgcacc
     4141 ggtttatgcg tccacacatc ggtatacggc acctgcaccg tcacgccaaa ataccgccgc
     4201 agatgtttat attcactcag cagctccgca tactgccggt tcagtgaggt atacgtatcc
     4261 accagctggt ggtggagctt ttccagttca ccacgccgat gcttctcttc tgccacccgg
     4321 gcaaacagcg cctgtaattt cagataatcg ctttcattcg gcaactgcca ctgaccggca
     4381 ctgaaccagt gcgacaccat gtttttcttt cctgtggcat ctgcaatctg ttttgccgtt
     4441 atccccaggg cagctcgcgc atcacgaaag taagaaatca gcggggccat cacatgctgt
     4501 ttcagtgcac tgcccttcgc cgcatacccg gcatctttcg gacgatacgg cccctgataa
     4561 tgttccgcga acagaatgcg ctctgtggcg gggaaatacg cccgcaggct ttccttgttg
     4621 catccgttcc agcgtccgga cggcttcgcc cagataatat ggttcagcac actgaagcgt
     4681 tcacgcatca tgatttcgat atcagatgcc aggcgatgac cacagaacag gtaaagactt
     4741 ccggcaggtt tcagcacccg ccagaactgc gccagacact ggtccagcca cttcaggtaa
     4801 tcatcgtcgc ccttccactg gttatcccag ccctcaggct tcactttaaa gtacggcggg
     4861 tccgtgacta tcaggtcaac agaattttcg ggtaacgacc ggataaattc caggcagtcg
     4921 gcgttgatta actcacaact ggatattttt acagtattaa gcatggatca ttaagcctgt
     4981 ctctgatagg ctcattctgc ttttgcgcaa agcagtgggc ctgaggtttg cttgtgaacc
     5041 caacgcatga gcagatggct ggtgggtgcc cctaacaccc accagccgcc catttaccac
     5101 aaataaaaaa gccttcactg cggaaggcgt ctgtaacaac cgaactgata gtctgccaga
     5161 cccgccataa ccagctgggt cagtattaac tggcagcgtt cgcgtgaaag gtaagtattc
     5221 tgcgctatct ccccgactgt cgccggttcg gtaacgctta attcattaaa caccactctg
     5281 gcggtttctg tcatatcctg ctgttttagc atgtcttttc cttttccggt taacgtgaca
     5341 caccaataac tcttgtcgaa aaagccagca agctgaaaga caggtattca ccgccaccag
     5401 cgcgtttact gtactgacgc gatttcagtc ataaaaaacc cgctcggcgg cgggttgtag
     5461 aaactcttct aacgtcaggc ataaaacgcc catcgttatg acgaatttac cacagattcc
     5521 ggaaaaatca accttgttac ctagttacct tttttaactg ccgctcagcc catgcttctt
     5581 caatatcaaa ccgggtcacc agcgcatcat aaaatttctt aactgttttt tcccatgacg
     5641 cgcgtgttat ctggtttgtc acctcgcata tagcattaaa tgcctccgtt gatggtagtc
     5701 tttcatagcc acgaccacca caacgctggc agtctctgat aacaggcata ccacgtttta
     5761 ccgactcttc acgatgaatg gcgacaccac gcccacggca atccttacag gcggtggaaa
     5821 cctcaccctt tccgccacac tccggacagg caacttttac cacctccctg acttttttcc
     5881 attcttccca gtaagacgga tacacacctt tcgtacactt tgcccatacc ggcggcttac
     5941 catccggata ctggatcttg tttgtaaaaa cctcgctttc aataaatttt tttccgtgac
     6001 agcaggggca ctgttttttg ctcgccgcgc tacgggcata atcttcaaac gcatacgaag
     6061 ccataatacg catcactgcc ggttttattt ctgccgggag ttttcttaac gccgccacgc
     6121 gatcacaccg actgagtgca tattctgtca gcaattctgt tgcccgctct ctgtcattca
     6181 tactaatgcc cattttccca aggaacgcag aaacccccat ctcagcccga ttctgtgtca
     6241 tgccctgcgc ggccatcaca tcagtgatac tcagcgcatc tttcgacgtt gaggccgatg
     6301 catcagtcag gccgggggat tttggggagt agtatttcgg taaatcttcc agtttcattt
     6361 tttgacctgc ccttcaagca ttatgggata aatcttcacc cccagacgtc caccagatac
     6421 tggctgagca cgaacgatat tgatttcatc aaactgctca tcgtccatta gcaaccccgc
     6481 atgcgtcagc gcatccagcg gcgctttcag aatattgtcc aggtcacggc ggcgcttatc
     6541 cggtggttct gcaataattt ttattgccaa ccttccggac aggcttaatt tcagccgctg
     6601 ctggcgaaca ataagcgcca ctgcccggcg ataacgctcc ccggcttttg atacaaaata
     6661 tgtgctgcca cgacgacgcc agtaagtgtt caccgtcggc gggtaaggta aaacaaattc
     6721 tatgcgctcg gtcatttatg ctttccactt cagaacaccc gaatttctcg cgtgcattaa
     6781 aaaacgaatc agcaacaaca gctgactgcc gtgtttctct tcaaaatctt ttactccggc
     6841 atgtagttcg ttacggcatt tacggcataa cggaataaca aacaaatcat cagcctttgt
     6901 tcccatccct cccagtccat gaccaatgat gtgatgcgga tcatctgcct gattgccaca
     6961 cgtcatgcat ttctgcgttt ttacccagcg cgtgtataca ggcatctctt cccgttgtga
     7021 tttctggcgc tggagatact gagccggtga ctccggatca acggcaatgc tgaccaccgt
     7081 cttttcctgt ggtgggttct gttgctggtg gacgtgaggc agtaacgcaa tattttttgt
     7141 gcgctgcttc agtatgctgg tggctgtctg ctctcccggt atgatgtcgc tttcactgta
     7201 cacagagcga attttttccg cacgcaaccc cagcgaacga cgtaataccg cttccggtag
     7261 cgcgtccgcc acctgattgc ggaccgccca ccaggataat tcagccagag ataattcacg
     7321 ctcctgcgta ccgcttattg cgtgaccgat gacgtcaatc atccatgctg acaggttttg
     7381 atgagcaagt tgctcgagtg attcggatgt ctggtcacgc agctggttgt cgcagtgcca
     7441 gcacaacacc attgcgccgg taccataacg gtgaatgacg gtttcgctgt gatgataatc
     7501 gccgtgtggc cactggcagg atttaatatg gcgcaacagc cagtcagaca atgcaccagc
     7561 accaccagca gcacgaatca cccgtgcgtt actgaaaaac ggcagcaatg ttttgtcttc
     7621 caccagcggc tggcgagcgg caggaacgac cccggacggc agattacgca tgctttttgg
     7681 ttccggctcc accagtattc tgccgttatg gaatgctgac attgattcac ggcctggctt
     7741 aacgataacc agaccgagtt ccggtaccag aacaggtcga agtaataccc gcac
//
LOCUS       DAKISA010000156         7710 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.158, whole genome shotgun sequence.
ACCESSION   DAKISA010000156 DAKISA010000000
VERSION     DAKISA010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7710
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.158"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..642
                     /locus_tag="OVW45_003964"
     CDS             <1..642
                     /locus_tag="OVW45_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCV5528295.1"
                     /translation="HAFITESDADGHVLTVREDLIWPLLEVLYPGTRETFGLPGICLS
                     LADKPDELAALEHYWQLIERESVEQLPGREHTLTLLAQAVFTLLLRNAKLDDHAASGM
                     RGELKLFQRFNMLIESHFHQHWTVPDYANELHITESRLTDICRRFANRPPKRLIFDRQ
                     LREAKRLLLFSDNAVNNIAWQLGFKDPAYFARFFNRLVGCSPSAYRAKKVPVT"
     gene            893..2451
                     /gene="hpaB"
                     /locus_tag="OVW45_003965"
                     /pseudo
     CDS             893..2451
                     /gene="hpaB"
                     /locus_tag="OVW45_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            2469..2981
                     /locus_tag="OVW45_003966"
     CDS             2469..2981
                     /locus_tag="OVW45_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCV5528296.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            3374..5524
                     /gene="btsT"
                     /locus_tag="OVW45_003967"
     CDS             3374..5524
                     /gene="btsT"
                     /locus_tag="OVW45_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCV5528297.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGHLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            5574..5777
                     /locus_tag="OVW45_003968"
     CDS             5574..5777
                     /locus_tag="OVW45_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCV5528298.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            5788..6744
                     /gene="yjiA"
                     /locus_tag="OVW45_003969"
     CDS             5788..6744
                     /gene="yjiA"
                     /locus_tag="OVW45_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCV5528299.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYEIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(6822..>7710)
                     /locus_tag="OVW45_003970"
     CDS             complement(6822..>7710)
                     /locus_tag="OVW45_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HCV5528300.1"
                     /translation="RVTEIDRLLEQKPRLTADQAWDVIRQTSRQDLNLRLFLPTLQAA
                     TSGLTQSDPRRQLVDTLTRWDGINLLNDDGKTWQQPGSAILNVWLTSMLKRTVVAAVP
                     MPFDKWYSASGYETTQDGPTGSLNISVGAKILYEAVQGDKSPIPQAVDLFAGKPQQEV
                     VLAALEDTWETLSKRYGNNVSNWKTPAMALTFRANNFFGVPQAAAEETRHQAEYQNRG
                     TENDMIVFSPTTSDRPVLAWDVVAPGQSGFIAPDGTVDQHYEDQLKMYENFGRKSLWL
                     TKPDVEAHKESQEVLHVQR"
BASE COUNT         1780 a         2102 c         2023 g         1805 t
ORIGIN      
        1 catgcgttta ttacggagtc tgacgccgac ggtcatgtgt tgacggtacg ggaagatctg
       61 atctggcccc tgctggaagt tctttatcca ggcactcggg aaaccttcgg cctgccgggg
      121 atttgcctgt cactggcaga taaacccgac gaactggcgg cgctggaaca ctattggcaa
      181 ctgatagagc gggaatcggt ggaacaactg cctggacggg aacacaccct gacgctactg
      241 gcacaggcag tgttcaccct actgctgcgt aatgcaaaac tcgacgacca tgccgccagc
      301 gggatgcgcg gagaattaaa actgttccag cgttttaata tgctgattga aagccatttt
      361 catcagcact ggacagtacc ggattacgct aacgaactgc atatcaccga atcacgcctc
      421 acggacatct gccgccgctt tgccaaccgt ccgccaaaac ggttgatttt cgacaggcag
      481 ctgcgagaag ccaagcggct gctgctgttt tctgataacg ccgtgaacaa tattgcctgg
      541 caactcggtt ttaaggatcc agcttatttt gcgcgctttt ttaatcgctt agtcggttgc
      601 tcgcccagtg cttatcgtgc caaaaaagta cctgtgacgt gaaaatccct tagccttaac
      661 gggaaaccag gcaccacctg ctattcccct ttctccgcct ggagcaaggg gatttagtaa
      721 cgtcaaaaat cgctaaaagc gaccgcgatc acaaaaccgt acccgacctg aaaagtacca
      781 gccattgtcc caaaagtgtc ttcccgtaat cgcccctgca agcttcaatt gaataacaaa
      841 aacaacacac aaaattaaca atcgtattcc gattaatact gtagaggtcg acatgaaacc
      901 agaagatttc cgcgccagta cccaacgtcc gttcaccggg gaagagtatc tgaaaagcct
      961 gcaggatggt cgcgagatct atatctatgg cgagcgagtg aaagacgtca ctactcatcc
     1021 ggcatttcgt aatgcggcag cgtctgttgc ccaactgtac gacgcgctgc acaaaccgga
     1081 gatgcaggac tctctgtgct ggaacaccga caccggcagc ggcggctata cccataaatt
     1141 cttccgcgtg gcgaaaagtg ccgacgacct gcgccagcaa cgtgacgcca tcgctgagtg
     1201 gtcacgcctg agctatggct ggatgggccg taccccagac tacaaagctg ctttcggttg
     1261 cgcactgggc gcgaatccgg gcttttacgg tcagttcgag cagaacgccc gtaactggta
     1321 cacccgtatt caggaaactg gcctctactt taaccacgcg attgttaacc caccgatcgt
     1381 catttgccga ccgataaagt gaaagacgtt tacatcaagc tggaaaaaga gactgacgcc
     1441 gggattatcg ttagcggtgc gaaagtggtt gccaccaact cggcgctgac tcactacaac
     1501 atgattggct tcggctcggc acaagtaatg ggcgaaaacc cggacttcgc actgatgttc
     1561 gttgcgccaa tggatgccaa tggcgtcaaa ttaatctccc gcgcctctta tgagatggtc
     1621 gcgggtgcta ccggctcgcc gtacgactac ccgctctcca gccgcttcga tgagaacgat
     1681 gcgattctgg tgatggataa cgtgctgatc ccatgggaaa acgtactgat ctaccgcgat
     1741 ttcgatcgct gccgtcgctg gacaatggaa ggcggttttg cccgtatgta tccgctgcaa
     1801 gcctgtgtgc gcctggcagt gaaactcgac ttcattacag cactgctgaa aaaatcactc
     1861 gaatgtaccg gcaccctgga gttccgtggt gtacaggccg atctcggtga agtggtggcg
     1921 tggcgcaaca ccttctgggc attgagtgac tcgatgtgtt ctgaagcgac gccgtgggtc
     1981 aacggggctt atttaccgga tcatgccgca ctgcaaacct atcgcgtact ggcaccaatg
     2041 gcctacgcga agatcaaaaa cattatcgaa cgcaacgtta ccagtggcct gatctatctc
     2101 ccttccagtg cccgtgacct gaacaatccg cagatcgacc agtatctggc gaagtatgtg
     2161 cgcggttcga atggtatgga tcacgtccag cgcatcaaga tcctcaaact gatgtgggac
     2221 gccattggca gcgagtttgg tggtcgccac gaactgtatg aaatcaacta ctccggtagc
     2281 caggatgaga ttcgcctaca gtgtctgcgc caggcacaaa gctccggcaa tatggacaag
     2341 atgatggcga tggttgatcg ctgcctgtcg gaatacgacc agaacggctg gactgtgccg
     2401 cacttgcaca acaacgacga tatcaacatg ctggataagc tgctgaaata acgcagcagg
     2461 aggttaagat gcaattagat gaacagcgcc tgcgctttcg tgacgcgatg gccagcctgt
     2521 cggcagcggt aaatattatc accaccgagg gcgacgccgg acaatgcggg attacggcaa
     2581 cggccgtctg ctcggtcacg gatacaccac catcgctgat ggtgtgcatt aacgccaaca
     2641 gtgcgatgaa cccggttttt cagggcaacg gtaagttgtg cgtcaacgtc ctcaaccatg
     2701 agcaggaact gatggcacgc cacttcgcgg gcatgacagg catggcgatg gaagagcgtt
     2761 ttagcctctc atgctggcaa aaaggtccgc tggcgcagcc ggtgctgaaa ggttcgctgg
     2821 ccagtcttga aggtgagatc cgcgatgtgc aggcaattgg cacacatctg gtgtatctgg
     2881 tggagattaa aaacatcatc ctcagtgcag aaggtcacgg acttatctac tttaaacgcc
     2941 gtttccatcc ggtgatgctg gaaatggaag ctgcgattta agctccagcc ccttcgcgca
     3001 ttcgtcgaag gggcattttt attgtgatct caatcacata tccacccgat gaataattct
     3061 tgcccaaccg ctcaccgcca ttcctgaccg cttacgtccc taaaataacc actcagttat
     3121 ttaccttact ttacgcgcgc gtaactctgg caacatcact gcaggatagc ggtcaattta
     3181 cctcctcaaa cgcaacgcaa acctagaacg gcttcggcct atgattaaca aattactcta
     3241 tgaattttgg tttgcaggaa ggcagcaagt gagtgaatcc ccgggagctt acaacagtaa
     3301 gtgacagggg tgaacgaacg caactgccgc acctgtaagc caaaagacga cgagtaaaat
     3361 gccaggtttt actatggata cgaaaaaact attcaagcac ataccctggg tgattctcgg
     3421 aatcatcggt gcattctgcc tcgcggtagt tgcattacgt cggggggagc acgtcagcgc
     3481 cctgtggatc gtggtcgcct ctgtgtcggt gtatctggtg gcgtatcgct actacagtct
     3541 gtacatcgcc cagaaggtga tgaaactcga ccccacgcgc gcgacgcctg cggttattaa
     3601 caacgacggt ctgaactacg ttccgaccaa ccgttacgtg ttgtttggtc accacttcgc
     3661 cgctatcgcc ggtgctggtc cgctggtcgg tccggttctc gccgcacaga tgggccacct
     3721 gcctggcacg ctgtggctgc tggcgggggt agtactggcc ggtgcggttc aggactttat
     3781 ggtgctgttt atctcctctc gccgtaacgg cgcatctctc ggtgagatga taaaagaaga
     3841 gatgggacca gtaccgggga ctatcgcgct gtttggctgt ttcttaatca tgatcatcat
     3901 ccttgccgtc ctggcgctga ttgtggttaa agccctggcc gaaagtccgt ggggtgtctt
     3961 caccgtttgc tcaaccgtac cgattgcgct gtttatgggt atctacatgc gctttatccg
     4021 tccggggcgt gtgggtgaag tctctgtcat tggtatcgtg ctgctggttg cctctatcta
     4081 cttcggtggc gtgattgctc acgatccgta ctggggtccg gcactgacct ttaaagacac
     4141 caccattacc ttcgcgctga ttggctatgc gtttgtttcc gcattgctgc cggtgtggct
     4201 gatcctcgca ccgcgcgact atctggcaac cttcctgaaa atcggcgtta tcgtcggcct
     4261 ggcgctgggt atcgtggtgc tgaacccgga actgaaaatg cctgcgatga cccagtacat
     4321 tgacggtact ggcccgctgt ggaaaggtgc tctgttcccg ttcttgttca ttaccatcgc
     4381 ctgtggtgcg gtatctggct tccacgcact gatctcttcc ggtacgacgc cgaaactgct
     4441 ggctaatgaa accgacgcgc gtttcattgg ctacggcgca atgctgatgg agtccttcgt
     4501 ggcgattatg gcgctggttg ctgcgtccat catcgaaccg ggtctttact tcgcgatgaa
     4561 caccccgcct gctggccttg gcatcaccat gcctaacctg catgaaatgg gtggcgagaa
     4621 cgcgccgatc atcatggcgc agctgaaaga cgttaccgca cacgcggcag cgaccgtcag
     4681 ctcttggggc ttcgtgattt cgccagagca gatcctgcaa accgcgaaag acattggtga
     4741 gccttctgtc ctgaaccgtg caggtggtgc gccaacgctg gcggtaggta tcgctcacgt
     4801 gttccacaaa gtgctgccga tggctgacat gggcttctgg tatcacttcg gtattctgtt
     4861 cgaagccctg ttcatcctga ccgcgctgga tgcgggtacc cgttctggcc gttttatgct
     4921 gcaagacctg ctgggtaact tcatcccgtt cctgaaaaaa accgactctc tggttgccgg
     4981 tatcatcggt actgcgggct gtgtgggtct gtggggctac ctgctgtatc agggcgtggt
     5041 cgatccgctg ggcggcgtta agagcctgtg gccgctgttc ggtatctcca accagatgct
     5101 ggcagccgta gcgctggtac tgggcaccgt tgtgctgatt aagatgaagc gcacccaata
     5161 catctgggta actgttgttc cggctgtatg gctgcttatc tgcaccacct gggcgctggg
     5221 tctgaaactg ttcagcacca acccgcagat ggaaggcttc ttctacatgg caagccagta
     5281 caaagagaag attgctaacg gtactgacct gacggcgcag cagattgcca acatgaacca
     5341 catcgttgtg aacaactaca ccaacgcagg cctgagtatt ctgttcctga ttgtggtgta
     5401 cagcatcatc ttctacggtt tcaaaacctg gcttgcggtg cgtaacagcg acaaacgtac
     5461 tgacaaagaa actccgtacg ttccaatccc ggaaggcggc gtgaagatct cttcgcacca
     5521 ctaaccgtgt ttagccccgc ttcggcgggg ctttgttctt atcagagtga actatgtttg
     5581 gtaacttagg acaggcaaaa aaatatctcg gccaggcggc gaagatgttg attggcattc
     5641 cagactacga caactacgtc gagcatatga agaccaacca tcccgacaag ccgtacatga
     5701 gctatgaaga attcttccgc gaacgccaga acgcgcgcta cggcggcgat ggtaaaggcg
     5761 gtatgcgctg ttgttaatgg agagagcatg aacccgattg cagttaccct actcaccggt
     5821 tttttaggcg cgggcaaaac caccctgctg cgccatattc ttaacgaaca acacggctac
     5881 gagattgccg tgattgaaaa cgaattcggc gaagtctccg ttgatgatca actgattggc
     5941 gaccgcgcca cgcagatcaa aacgctgacc aacggctgca tctgctgttc gcgctccaac
     6001 gagctggaag acgcactact cgacctgctg gataatctcg acaagggcaa tattcagttc
     6061 gaccgtctgg tcattgaatg caccggcatg gccgatcccg gcccgattat tcagaccttc
     6121 ttctcccatg agattttatg ccagcgctac ctgctggacg gggtgattgc gctggtggat
     6181 gcggtacatg ccgatgagca gatgaaccag ttcaccatcg cccagtccca ggttggctac
     6241 gccgaccgta ttctgctgac gaaaaccgac gtcgcaggcg aagcagagaa actgcgtgaa
     6301 cgcctggcgc gcatcaacgc ccgcgcaccg gtctacaccg ttacccacgg cgatatcgac
     6361 ctgggccttc tgttcaacac caacggtttt atgctggaag aaaacgtcgt cagcaccaaa
     6421 ccgcgtttcc actttatcgc ggacaaacaa aacgatattt cgtcgattgt ggtggaactg
     6481 gattacccgg tagatatcag cgaagtttcc cgggtgatgg aaaacctgct gctggaatcg
     6541 gcggataaac tgctgcgtta caaagggatg ctgtggattg acggcgaacc taaccgcctg
     6601 ctgttccagg gcgtccagcg cctctacagc gccgactggg accgcccgtg gggcgatgaa
     6661 aaaccgcata gcacgatggt gtttatcggg attcagttgc cggaggatga gattcgggcg
     6721 gcgtttgcgg gactcaaaaa gtaaccagcc ctccaacatc acaatacctg agactttatc
     6781 accctcatat tcctccagaa tatgagggca ttcgggctta attatctctg aacgtgcaac
     6841 acttcctgcg actccttatg cgcctccaca tccggcttcg ttaaccagag cgacttacga
     6901 ccaaaatttt cgtacatttt cagctgatct tcatagtgct gatcaactgt tccatcggga
     6961 gcaataaacc cactctgacc gggtgcgacc acatcccagg caagcaccgg acgatcgctt
     7021 gtcgttggtg agaaaacaat catatcgttt tctgttccac ggttttgata ctccgcctga
     7081 tgacgcgttt cttccgctgc ggcctgcggt acaccaaaga aattatttgc acggaacgtt
     7141 aaggccattg caggtgtttt ccagttactc acattattgc catagcgttt ggaaagagtc
     7201 tcccaggtat cttccagcgc agccaacaca acctcctgct gtggtttccc agcaaacaga
     7261 tcaaccgcct gtgggattgg tgatttgtct ccctgcaccg cctcatacaa aatttttgct
     7321 ccaacactta tattcagcga accagttggg ccgtcctggg ttgtttcgta gccactggcg
     7381 ctgtaccact tatcaaatgg cataggtacg gcagccacta cggtacgctt caacatactg
     7441 gtcagccaaa cgttcaggat ggcagagcct ggctgctgcc aggttttacc atcatcatta
     7501 agcaaattga tgccatccca acgtgttaat gtatccacca actgacggcg cggatcgctc
     7561 tgtgtcaaac cagatgtcgc tgcttgcaga gtaggtaaaa aaagcctcag gttaagatcc
     7621 tgacgactgg tttggcgaat aacatcccat gcctgatcag cagttaagcg tggcttttgc
     7681 tcaagcagtc ggtcgatctc cgtaacgcga
//
LOCUS       DAKISA010000157         7611 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.159, whole genome shotgun sequence.
ACCESSION   DAKISA010000157 DAKISA010000000
VERSION     DAKISA010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7611
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.159"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            171..701
                     /locus_tag="OVW45_003971"
     CDS             171..701
                     /locus_tag="OVW45_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCV5528301.1"
                     /translation="MNDKELIKEIKERISSLDVRDNVERRAYEIALASLTAEPVAWKV
                     TFTQIDREYNTFTGMYSDKAEVERWVRLHKACNFRADITPLYTAQPVPVTPDAWISCS
                     ERMPEMGERQCYVLAADFKNNYPPNIPNTQVGVYGDWFNDGNPTWDDGDGEDLYLKEV
                     THWMQLPEPPQEVNRG"
     gene            780..1502
                     /locus_tag="OVW45_003972"
     CDS             780..1502
                     /locus_tag="OVW45_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528302.1"
                     /translation="MVNNIANHSSFPAVTIDSQMLLKMVNEARKLCGEPSVRNNKFIE
                     KIEDELESETYTKSVGRKNGADIDVISMTIKQALRVAARESKAVRRTLVDKLESMQEA
                     HIKSGKSASGLVEYRQARTLKMTVEAVTNLFDLMPNLAPEAKQTAAASIINPLVGFNA
                     IPLPAIEEHYYSAGEVAEQLGVTANKIGRIANANNLKTEQYGKFFLDKSAHSSKQVET
                     FRYNEAGVHKIEELIEGERKAA"
     gene            1824..2447
                     /locus_tag="OVW45_003973"
     CDS             1824..2447
                     /locus_tag="OVW45_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HCV5528303.1"
                     /translation="MTSQLIPVFNGTISNETTLLVNARDLHEFLEVRRDFSTWIKNRI
                     TEYGFAVDVDYILVHQNRGIKGRGGDRRSKDYHLTLDTAKETAMVERNEKGRQIRRYF
                     IECEKKLRSMQPAQQFTDEEIILLCYMQVQMENAQDICKRLYPILKELNSSYTSKLYD
                     IAFETFYTVTKNRDVLLREATRLDQASALFERAKPMLKSLRARQFEF"
     gene            2532..2750
                     /locus_tag="OVW45_003974"
     CDS             2532..2750
                     /locus_tag="OVW45_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCV5528304.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            2728..3798
                     /locus_tag="OVW45_003975"
     CDS             2728..3798
                     /locus_tag="OVW45_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCV5528305.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTKPVAATRAAKSEVRRSRLTADEYLKIYQTAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            3933..5216
                     /locus_tag="OVW45_003976"
     CDS             3933..5216
                     /locus_tag="OVW45_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCV5528306.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNMYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(5450..>7611)
                     /locus_tag="OVW45_003977"
     CDS             complement(5450..>7611)
                     /locus_tag="OVW45_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCV5528307.1"
                     /translation="GTIAWSILSSHNTSGNMDKLKIKFDSLASHDITFVGIVQTAKAS
                     GMERFPLPYVLTNCHNSLCAVGGTINGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMRE
                     MMAGGGKMILGSDSHTRYGALGTMAVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKP
                     APYVGPQDVALAIIGAVFKNGYVKNKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSS
                     VWQTDEEVHNWLALHGRGQDYCQLNPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYE
                     IDTLNQNLTDILREIEIESERVAHGKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENV
                     IAAANALRGQSCGNDTFSLAVYPSSQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFG
                     AGDTPINNGLSIRHTTRNFPNREGSKPANGQMSAVALMDARSIAATAANGGYLTSASE
                     LDCWDNVPEYAFDVTPYKNRVYQGFVKGATQQPLIYGPNIKDWPELGALTDNIVLKVC
                     SKILDEVTTTDELIPSGETSSYRSNPIGLAEFTLSRRDPGYVGRSKATAELENQRLAG
                     NVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQRVIGGLAN
                     IAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIH
                     EDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
BASE COUNT         2095 a         1863 c         1894 g         1759 t
ORIGIN      
        1 cgctacgcga taagcaggcg ttacttgaag cctcagaaaa gcgcatagca gaactggagg
       61 cacagacagt taccgttaaa gaggttggag atgcttgata gttttgcgct cgttgcactg
      121 gttggcattg tagcgttgat ttgggtgaca tccgggaatc ggaggcataa gtgaacgaca
      181 aagaactgat taaagaaatc aaagagcgta tcagcagttt ggatgtgcga gacaatgttg
      241 aacgccgtgc ttatgaaatt gctctggcat cgctgacagc agagccggtg gcgtggaagg
      301 taaccttcac gcaaattgac cgtgaatata acacgttcac tggtatgtat tctgacaaag
      361 cagaagtcga acggtgggtg cggctgcata aagcatgtaa ttttcgggca gatataacac
      421 cgctttatac cgcccagcca gtgccggtaa ctccggatgc ctggataagc tgtagtgagc
      481 gaatgccaga aatgggagag cgacaatgct atgtgttagc agctgacttt aaaaacaact
      541 acccaccaaa catccccaac actcaggtcg gcgtatatgg cgactggttt aatgatggca
      601 atcccacttg ggatgacggt gatggcgaag acctgtatct caaagaggta acccactgga
      661 tgcagctacc agaaccgccg caggaggtta accgtggcta acctgcaact tgccgtcaaa
      721 ggtgaataac aatcctcgca ctcgcgggga tttcttttat acgatatagg ggaaaaggtg
      781 tggttaacaa tattgcaaat cactcttcgt ttccggctgt aacaatcgac agccagatgc
      841 tgttgaaaat ggtcaatgaa gctcgcaagt tatgtggaga gccatcagta cgtaacaaca
      901 aattcatcga gaaaattgag gatgaattgg aaagcgagac ctacacaaaa agtgtaggtc
      961 ggaaaaacgg ggctgacatt gatgttatct ccatgactat caagcaggcg cttcgtgttg
     1021 ctgctcgcga atctaaagca gttcgccgaa cacttgtaga caaacttgaa agtatgcagg
     1081 aagcgcacat taaaagcggt aaatcagcga gtggacttgt tgagtatcgt caagcgcgaa
     1141 cactgaaaat gacggttgaa gctgttacca atctgttcga tttgatgcca aatcttgcgc
     1201 cggaagcaaa gcagactgct gcggcaagca taatcaaccc gctcgttggt tttaatgcaa
     1261 tacctcttcc ggcaatagaa gagcattact actcagcagg ggaggtcgca gagcagctcg
     1321 gagtaacggc aaacaagatt ggtcgcattg ctaacgcaaa caacctcaaa actgagcagt
     1381 acgggaagtt cttcctggat aaatctgcgc attccagcaa gcaggttgaa acatttcggt
     1441 acaacgaggc tggagtgcac aaaatagagg aactcatcga aggagagcga aaggctgcat
     1501 gattttgaca agatagtttt ccccaaatgt ggggaaaagc ccgaatggcg cggcttacag
     1561 caagataagg cctacatgat ttgacaacac cgcattaacg gggctatatt ccgcttcatg
     1621 gtgctgaaca caccttgcaa agcggaaacc gcacccgtca gtcatgcgga ttttttatgt
     1681 ccatttttca gatatggtcg ggtagcgcgt ataccgaaaa acagccgaaa ggttaaggat
     1741 acgggccgac tttgcacggt gttcaagtac ctgaccgccc tgctgaacac agggctatct
     1801 gaacaaatgc aaaggacata aaaatgacca gtcaactcat ccccgtattc aacggcacta
     1861 tatccaacga aacaactctt ctcgttaatg cccgtgattt acacgaattt ttggaagtac
     1921 gtcgtgactt ctctacatgg attaaaaatc gcataacaga atacggcttt gccgttgatg
     1981 tcgattacat tttggttcac caaaacaggg ggatcaaagg acgtggaggc gatcgccgta
     2041 gcaaagacta tcacctcacc ctcgatacag ccaaagaaac ggcgatggtc gagcgtaacg
     2101 aaaaaggccg ccagatacgc cgatacttca tcgagtgcga aaagaaactt cgcagcatgc
     2161 aaccagcgca gcaattcaca gacgaagaaa tcatcctcct ttgctatatg caggtacaga
     2221 tggagaatgc gcaggacatc tgcaaacgtc tgtacccgat attgaaggaa ctgaactcat
     2281 catacacgag caagctgtat gacatcgcgt ttgaaacttt ttacacggtg acgaaaaaca
     2341 gagatgtact gctaagggag gcgacacgac ttgaccaagc aagcgccctt ttcgaacggg
     2401 caaaaccaat gctgaaaagc cttcgggcga gacaattcga attttaatca tcaaaggagc
     2461 ttcggctcct tttttgttgg agaaaataaa ccaatactcg ctcccttgcg agtaattgcg
     2521 gagactttgc gatgtacttg acacttcagg agtggaacgc acgccagcga cgcccaagga
     2581 gccttgaaac agttcgtcga tgggtacgcg agtgcaggat attccctcct ccggttaagg
     2641 atggaagaga gtatttgttc cacgaatcag cggtaaaggt tgacttaaat cgaccagtaa
     2701 caggtagcct tttgaagagg atcagaaatg ggaagaaggc gaagtcatga gcgccgggat
     2761 ttacccccta acctttatat aagaaacaat ggatattact gctacaggga cccaaggacg
     2821 ggtaaagagt ttggattagg ccgagacagg cgaatcgcaa tcactgaagc tatacaggcc
     2881 aacattgagt tattttcagg acacaaacac aagcctctga cagcgagaat caacagtgat
     2941 aattctgtta cgttacattc atggcttgat cgctacgaaa aaatcctcgc cagcagagga
     3001 atcaagcaga agacactcat aaattacatg agcaaaatta aagcaataag gagggggctg
     3061 cctgatgctc cacttgaaga catcaccaca aaagaaattg cggcaatgct caatggatac
     3121 atagacgagg gcaaggcggc atcagccaag ttaatcagat caacactgag cgatgcattc
     3181 cgagaggcaa tagctgaagg ccatataaca acaaaaccgg tcgctgccac tcgcgcagca
     3241 aaatcagagg taaggagatc aagacttacg gctgacgaat acctgaaaat ttatcaaaca
     3301 gcagaatcat caccatgttg gctcagactt gcaatggaac tggctgttgt taccgggcaa
     3361 cgagttggtg atttatgcga aatgaagtgg tctgatatcg tagatggata tctttatgtc
     3421 gagcaaagca aaacaggcgt aaaaattgcc atccctacaa cattgcatgt tgatgctctc
     3481 gggatatcaa tgaaggaaac acttgataaa tgcaaagaga ttcttggcgg agaaaccata
     3541 attgcatcta ctcgtcgtga accgctttca tccggcacag tatcaaggta ttttatgcgc
     3601 gcacgaaaag catcaggtct ttccttcgaa ggggatccac ctacctttca cgagttgcgc
     3661 agtttgtctg caagactcta tgagaagcag ataagcgata agtttgctca acatcttctc
     3721 gggcacaagt cggacaccat ggcatcacag tatcgtgatg acagaggcag ggagtgggac
     3781 aaaattgaaa tcaaataatg attttatttt gactgataat gacctgttcg ttgcaacaaa
     3841 ttgataagcg atgctttttt ataatgccaa cttagtataa aaaagcaggc ttcaacggat
     3901 tcatttttct atttcatagc ccggagcaac ctgtgaacac attttcagtt tcccgtctgg
     3961 cgctggcatt ggcttttggc gtgacgctga ccgcctgtag ctcaaccccg cccgatcaac
     4021 gtccttctga tcaaaccgcg cctggtacct cttctcgccc gattctgtcg gcaaaagaag
     4081 cgcagaattt cgatgctcaa cactattttg catccctgac accaggtgct gcagcgtgga
     4141 atccttcccc gattaccctg cctgcgcaac ctgactttgt tgtcggcccg gcgggcactc
     4201 aaggtgtaac gcataccacg attcaggcgg cggtagatgc ggcaattatc aagcgtacca
     4261 acaagcgcca gtatattgcc gtgatgcctg gtgagtatca gggaacggta tatgtccctg
     4321 ccgctccggg tggaattact ctgtacggta caggtgaaaa accgattgat gtgaagattg
     4381 ggctttccct tgatggtggc atgagccctg ccgactggcg tcacgacgtc aacccgcgcg
     4441 gcaaatatat gccaggtaaa ccagcgtggt atatgtacga tagctgccag agcaaacgca
     4501 gcgacagtat cggtgttctc tgctctgcgg tcttctggtc acaaaacaat ggcctgcaac
     4561 tgcaaaatct gaccatcgaa aacacgctgg gcgatagcgt agatgcaggt aaccatccgg
     4621 cggtggcact gcgtactgat ggtgaccagg tacagattaa caacgttaac attctcggtc
     4681 gtcagaacac cttctttgtc accaacagcg gtgtgcagaa ccgtctggaa acgaatcgtc
     4741 agccgcgtac gctggtgacc aacagctaca ttgaagggga tgtggatatc gtttctggtc
     4801 gcggcgcagt ggtgttcgat aacaccgaat tccgcgtggt gaactcacgt actcagcaag
     4861 aagcgtatgt gtttgcaccg gctacgctgt ccaacatgta ctacggtttc ctcgccgtaa
     4921 acagccgttt caatgctttc ggtgatggtg tggcgcaact gggccgctcg ctggatgttg
     4981 atgccaatac caacggtcag gtggtgatcc gtgatagcgc catcaacgaa ggttttaaca
     5041 cggctaaacc gtgggccgat gcggtgatct ctaatcgtcc gtttgcgggt aataccggca
     5101 gcgtagatga taacgacgaa atacagcgca atctgaatga cactaactac aaccgcatgt
     5161 gggaatacaa taaccgcggc gtgggtagta aagtggttgc agaggcgaag aagtaagagc
     5221 aattaactat ttgccggatg cggcgtaaac gccttatccg gcctacggtt cgatgcgatt
     5281 tgtaggtcgg ataagatgcg caagcatcgc atccgacaat aagtgccgga tgctgcgtaa
     5341 atgccttatc tggcctacag attcgatgcg attcgtaggt cggataagat gcgcaagcat
     5401 cgcatccgac aataagtgcc gaatgcgacc tacattcaca tggcgctttt tacatctgac
     5461 ggtttttatt gaagttaatc aaactacccg ccttgataat ctcgcgctct tcagcagtca
     5521 gactttccat atagagcgta atttccgtta ccggcgcatc ttcatggatc acataacctt
     5581 taaacgtcgt acccggatta tccagcgccg ctttaatgcc agggatgtaa atgtaatccc
     5641 ccacttcaaa ggttggcact tccgccatct gcagaggtaa catcccccag ttgatgacgt
     5701 tagaacggta gcgtttagtc gcgtactcct cggcaatatt cgccagaccg ccaatcacac
     5761 gctggcagct cgccgcctgt tcacgcgcag aaccatcgcc tggtttcacc gcatagacca
     5821 tgctgccaat ttcagtttgc agcggatcaa tatgctcctg accagcaatc tgcttaatgc
     5881 gcgcaaacac ctctgtcagc tcgctgacat tccccgccag acgctgattt tccagctcag
     5941 cagtcgcttt acttctgcca acataaccgg gatcacggcg agacagggta aactccgcca
     6001 gaccaatcgg atttgaacga taagaagagg tttcaccgga aggaatcagt tcgtcggtgg
     6061 tggtcacttc gtcgaggatc ttcgagcaca ctttcaggac gatattgtca gtcagcgcac
     6121 ccaattccgg ccagtcttta atgttcggcc cgtaaatcag cggttgctga gttgcccctt
     6181 tcacaaagcc ctgataaaca cggtttttat acggcgttac atcgaaggcg tactccggca
     6241 cgttgtccca gcaatcaagt tcgctggcag aggttaaata gccaccgttt gccgcagtcg
     6301 cagcgataga acgagcgtcc atcaacgcca ccgctgacat ctgcccatta gctggcttag
     6361 agccttcgcg gttcgggaag ttacgcgtgg tgtggcgaat actcaaaccg ttgttgattg
     6421 gcgtatcgcc cgcgccaaag catgggccgc agaacgcggt tctgatgatt gcgcctgcgc
     6481 caatcaaatc tgctaccaca ccttttttgg cgagatccat aaacaccggc tgtgatgacg
     6541 ggtaaactgc cagcgagaag gtgtcattgc cacaggattg accgcgcagt gcattcgccg
     6601 ccgcgatgac gttttcgtag ttaccgccag aacagcccgc gataatcccc tgctgcactt
     6661 tcaggcgacc attttccact ttatccagca gcgagagttt ggctttaccg tgcgccacgc
     6721 gttcggactc aatttcaatc tcacgcagaa tgtcggtcag gttctggttc agtgtgtcga
     6781 tttcatacac gttgctcggg tggaacggca gcgcaatcat tggtttgatg gcgcttaaat
     6841 caacgctgat gcagccatcg tagtacgcca tcggttgagg gttaagctgg cagtaatcct
     6901 ggccgcgacc gtgcagcgcc agccagttat gcacttcttc atcagtttgc cagacggagc
     6961 ttaaacaggt ggtttccgtg gtcatcacgt caacgctgtt acggaaatcg gtagagagcg
     7021 cgctaacgcc cggtccaacg aactccatga ctttgttttt gacgtaaccg tttttgaaca
     7081 ccgcgccaat gatagccagc gccacatcct gtggccccac atacggcgca ggttttccgg
     7141 tcagatgcac cgcaaccacg cccggatagt cgatatccca ggtgtcatta agcagctgtt
     7201 ttaccaactc accgccaccc tcaccgactg ccattgtccc taatgcaccg taacgggtgt
     7261 ggctgtctga cccgaggatc attttgcccc cgcctgccat catctcacgc atatattgat
     7321 ggatgaccgc aatatgcgga ggcacaaaaa taccgccata acgctgagct gccgataaac
     7381 caaaaacatg gtcatcaccg ttaatggtgc cgccgacggc gcagagtgag ttatggcagt
     7441 tggtcagcac atacggcagc gggaaacgtt ccattccgga cgctttagcg gtctgtacaa
     7501 taccaacaaa ggtaatatca tgagaggcta atgaatcaaa cttaatttta agtttatcca
     7561 tatttccgga cgtattatga gaagagagaa tagaccaggc aatagtgcct t
//
LOCUS       DAKISA010000158         7610 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.160, whole genome shotgun sequence.
ACCESSION   DAKISA010000158 DAKISA010000000
VERSION     DAKISA010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7610
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.160"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(51..3953)
                     /gene="hrpA"
                     /locus_tag="OVW45_003978"
     CDS             complement(51..3953)
                     /gene="hrpA"
                     /locus_tag="OVW45_003978"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCV5528308.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDVQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKIRKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQVMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            4154..4759
                     /gene="azoR"
                     /locus_tag="OVW45_003979"
     CDS             4154..4759
                     /gene="azoR"
                     /locus_tag="OVW45_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCV5528309.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(4810..6126)
                     /locus_tag="OVW45_003980"
     CDS             complement(4810..6126)
                     /locus_tag="OVW45_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HCV5528310.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDSNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGYCKTVDEAISFIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(6116..>7610)
                     /locus_tag="OVW45_003981"
     CDS             complement(6116..>7610)
                     /locus_tag="OVW45_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HCV5528311.1"
                     /translation="QDVDEFVRFAASDSQVGLEEVVVIAQSVGAVLVATWVHDYAPAI
                     RGLVLASPAFKVKLYVPLARPALTLWHRLRGLFFINSYVKRRYLTHDRQRVASFNNDP
                     LITRAIAVNILLDLYKTSERIIRDAAAITLPTQLLISGDDYVVHRQPQIDFYQRLRSP
                     LKELHLLPGFYHDTLGEENRAQAFEKMQSFIRRLYANKSQKFDYQHEDRTGPSADRWR
                     LLSGGPVPLSPVDLAYRFMRKAMKLFGTHSAGLHLGMSTGFDSGSSLDYVYQNQPQGS
                     NAFGRLIDKIYLNSVGWRGIRQRKTHLQILIKQAVADLHVKGLAVRVVDIAAGHGRYV
                     LDALANEPAVSDILLRDYSELNVAQGQEMIAQRGMSGRVRFEQGDAFNPEELSALTPR
                     PTLAIVSGLYELFPENEQVKNSLAGLANAIEPGGILIYTGQPWHPQLELIAGVLTSHK
                     DGKPWVMRVRTQGEMDLLVHDAGFDKCTQRIDEWGIFTVSMAVRRDN"
BASE COUNT         1847 a         2126 c         1831 g         1806 t
ORIGIN      
        1 ccggcgctaa atgcttaccg ggttttttct ttatcaggca aatagcaggg ttaaccgcta
       61 atctgctcca tcgcctgcaa aatacgctta tctgaaatcg gataaggcgt accaagttgt
      121 tgagcgaagt aactaacacg caactcttct atcatccaac ggatctcttt cacgtcttca
      181 tcctcacgac gtgcaggcgg cagtttgttg atccattgct gccacgcctg ctggacgttt
      241 tcgactttca gcatctgagc acggtcgcga tgtggatcaa ccgccagttt ttccagccgt
      301 ttttcaatcg cctgcaaata tcgcagcgtg tcgcccagcc gtttgaagcc gttaccagtg
      361 acaaaaccgc gatataccag cccgcccatc tgcgctttaa tgtcagaaag ccccagcgcc
      421 atcgtcatat ccacccgccc cttcagacgt ttgttgatat tgaacactgc cgtaaggatt
      481 tgctcgacct gcttcgcaat atccaccacc gtgtcgttca gttcggcacg cactttttca
      541 tgcagcgcag caaagccttc ttccgtccag accgggccac cattggcgtc gatcaattta
      601 tccacaccgc aggagataca gtcgtcgatc agctccagca ctttgccata cgggttaaag
      661 tacagcccca gcttggcttt gtttggtaac ttctcatgta aatacttgat cggcgaggga
      721 atattcagca gcagtagacg gcgaagaccg ttccacatta cctgtttttg ctccagcgga
      781 ttatcaaaca gtttgatcgc cacactgtcg cgttcatcca ctagcgctgg ccacgccttc
      841 actttgtagt tgccacgctt ctgttcgtag ctttccggca gctgaccaaa actccagata
      901 tgcagtccgc tctgctcgat accgtcatcc gccaccgcag acagcgtttc ctgcactttg
      961 cctttcagcg catctttcag atcttgtagc gagcgccctt cttttagctt cttgtttttg
     1021 tcatccacca cgcggaaggt gattttcagg tgatcgggca cctgatccca gtgccagtct
     1081 tcgcggtcaa cggtaacgcc ggtcatccgc cgtaactcgc gctcaaggct gtcgagcaac
     1141 ggtaactcca gcggtgtgac gcggcctaaa aacgcttcgg cgtagtttgg tgcaggcaca
     1201 aaattacggc gtaccggttt cggcaacgat ttaatcagag caatcaccaa ttcgcggcgc
     1261 agaccgggga tctgccattc aaacccgctt tcctcaacct ggttaagtaa cggcagcgga
     1321 atatgtacgg tcacaccgtc cgcgtccgcg cctggctcaa actgatagct caaacgcagc
     1381 ttgagattgc cctgatgcca gaagttcggg taatccagct ttctgatttt ttccgcccct
     1441 tctttgatca acatgctctt ttcaaagttg agcaaatcag gcgtttcgcg gctgactttt
     1501 ttccaccagc tatcgaagtg acgagcggag attacatcgt ggctgatgcg ctggtcgtag
     1561 aactcaaaca gcgtttcgtc atcaaccaga atatcgcggc gacgtgattt gtgttccagc
     1621 tcttccactt cggcccgcag tttcaggttt tcacggaaga atgcgtgacg cgtctgccag
     1681 tcaccttcca ccagagcgtg gcgaataaag agttcacgac ataacgccga atcgatctgg
     1741 ctgtagttga ccttgcgcgc agcaacaatc ggcaaaccat aaacagtgac tttttccgtt
     1801 gccatcaccg cgccctgcgc ccgttcccag tgcggttcgc tgtaggtgcg tttaatcaaa
     1861 tgctgagcaa cgggttctac ccattccggg tcgatacgcg cagcaatgcg cccccacagg
     1921 cggctggttt ctaccagttc cgccaccatt acccatttcg gcggcttttt gaataaacca
     1981 gaaccgggga agatggagaa acgcgcgtta cgtgcaccgg tatattcctg tttatcggca
     2041 tctttcatgc cgatatggga aagcaaaccg gtcagtaagg cgatgtgaat ttcacgatac
     2101 tccgccggtt cgctgttgac cggaatgcca agttctttca ccacctggcg caactgggtg
     2161 tagatatcct gccattcgcg cacgcgcaga tagttgaggt aatcggtacg acacaggcga
     2221 cggaaggcgt tggaagaaag cgccttttgc tgctcgccaa gataattcca cagatttaca
     2281 aacgcgagga agtcggattc tttgtcgtgg aagcgacgat gtttttcgtc cgatgcctgc
     2341 tgtttgtcca tcggacgttc gcgcggatcc tgaatggaga gcgcggacgt gataatcatc
     2401 gcctcacgca cgcagccatg tttttgcgct tccagcacca tacgcgccag acgtgggtcg
     2461 acaggcaact gcgagagctg gcgaccgagc ggcgtcagtt tataggcgct ggcctgttca
     2521 tccgtggtga tcgcgcccag ttcttcgagc agacgcacgc catcctggat attgcgttta
     2581 tccggtgctt cgacaaacgg gaacgcagcg atatcgccca gccccagcgc ggtcatctgc
     2641 aaaataaccg aggccaggtt ggtacgcaga atctccggat cggtaaactc cgggcgcgag
     2701 aggaaatcgt cttcggaata gagacgaata cagatccctt cggacacacg accacagcgg
     2761 cctttacgct gattggcaga cgcctgcgaa atgggttcaa tcggcaaacg ctgcactttg
     2821 gtgcgatagc tgtagcggct gatacgcgct gtaccggggt cgatgacgta tttaatcccc
     2881 ggtacggtca gcgaggtttc cgcgacgttg gtcgccagca caatgcgccg tccgctgtgc
     2941 gactggaaca cgcggttctg ttcgctgttc gaaagccgcg cataaagcgg caagatttcg
     3001 gtatggcgta agttcagctt gttcagcgca tcggcggtat cgcgaatttc ccgctcacca
     3061 ctcataaaga tcagaatgtc gccagggctt tcctgactca gttcgtctac ggcgtcaaaa
     3121 atcgcctgca actgatcgcg ctcggtgtca tcggcttctt caacaatcgg tcgatagcgc
     3181 acttccaccg gataggtccg accggagact tcgataatcg gcgcattatt aaagtggcgc
     3241 gaaaagcgtt ccgggtcgat agtcgcggaa gtgataatga tttttaggtc aggacgccgc
     3301 ggcagcaact ctttcaaata gccgagcaga aaatcgatat tcaggctgcg ttcgtgcgct
     3361 tcgtcaataa tgatggtgtc gtactgcatc agcaggcggt cttgctggat ctccgccagc
     3421 aggataccgt cggtcatcag cttgaccatc gtgttatcac ttacgtgatc gctgaaacgc
     3481 actttgtaac cgatgcaacc gcccggctcc gttttcagct cttccgcaat acggttcgcc
     3541 accgttcttg ccgccaggcg acgcggctgg gtatggccga tcagcccttt aatcccgcgc
     3601 cccagctcca tacagatttt cggtaactga gtcgttttac cagaacccgt ttccccggcg
     3661 acgatcacca cctggtgatc acgaatcgct tcgagaatgt cctgtttttt ctgactaacc
     3721 ggtaaattgt caggataagt aatttccggt cgtgccgctt cacgcagcag gactttccct
     3781 gccgcctggt caatctcttt cgccatctcc tggaaaatgg cctgttgtac atcaggattt
     3841 ttaaccttct tcacgccgtg cagacggcga gaaaaacgca gtctgtcacg cagcatcagc
     3901 gaatccagcc gctgctgcaa ggccgtaaag gtcaattttt gttgttctgt cataacgtta
     3961 gtgggcagta gtctgcctga ttaattttct ttgaaatgat agatatagag taccacatca
     4021 cggctttcta tgcgttgttc aataaattcg aacataggct tcgatttatt gcgctatctc
     4081 tgtggcagga ttgtgaataa agtgtcaaca agcaacgggg catcgcccat tcaaacatct
     4141 ataaggaaac accatgagca aggtattagt tcttaaatcc agcatcctgg cagggtactc
     4201 tcagtctaat cagttgtccg attattttgt tgaacaatgg cgcgaaaagc actccgctga
     4261 tgaaatcacc gttcgcgacc tggctgcaaa tccgattccg gtactggatg gcgaactggt
     4321 tggtgcactg cgtccgagcg atgcgccgct gactccgcgt cagcaggaag ctctggcgct
     4381 ttccgatgag ctgattgccg agctgaaagc ccacgacgtt atcgttattg cggcaccgat
     4441 gtataacttc aacatctcaa ctcagttgaa aaactatttt gacctggttg cacgcgcagg
     4501 cgttactttc cgctataccg agaacggtcc ggaaggtctg gtaacgggta aaaaagccat
     4561 cgttattacc agccgcggtg ggatccacaa agatggacca acggacctgg tgacgccgta
     4621 tctgtccacg ttcctcggct ttatcggcat tactgatgtg aaatttgtct tcgccgaagg
     4681 gatcgcatac ggtccggaaa tggcagcgaa agcgcagtct gacgcgaaag cagccatcga
     4741 cagcattgtt gctgcataac cggacacgat cttgatgtgg actgtagtcc agtccacatc
     4801 ccaatccact tacctgtttt cccataattt cagcatcgct ttgtgatctt ccttaagcac
     4861 aatatgcgag cgtctggctc gaataaagct aatcgcttca tcaacggttt tacagtatcc
     4921 gtaacataac agccatgccg ccactaccag cgcactgcgc gataatccca gtgcgcaatg
     4981 gaccagaacg ctgccttgct cttcgcgtaa tgtttccagc atcgccacgg cctgtcggag
     5041 ctccccctct tccggaacca ccagatctag catcggtaca cagaaataga gtcgatcttt
     5101 tgtcgctcgt ccacgaggga attcaaaggt gacgtccaga accgcattct gtgccggaat
     5161 atgtcgtgga aacgccccta aataaacacc agcagtcatt ttgctcaccg gctccaggcg
     5221 acgacaaaac caacgcatag atagccacat cccgatgcgc caaggcaatg tcagccagta
     5281 aacggcgggc gggagtttcc cctgactatc tttccctgtt gttatcgcgc caagcccacc
     5341 gtacccacgg ccaataatga gtagcgataa tactggccaa cataaccaga ctgaccacca
     5401 taactgaacc atcatcatta gctccatcaa cacaatgcac gcgcacgcgc ctacgacata
     5461 tggcagtgct attttgattc gacgttgatc aggtttctga taattccaac gacggtcgac
     5521 gggcaccatc cagtcaatca acatacctac cgccagccct gtgatgacat caataaaatg
     5581 atgctgccat gtcgtcagcg tcgaaatggc gatgagtaaa aaccatccgc cgcagacttt
     5641 acgccacctc acagccagat gctgacgaaa gtgacgccaa agtagccagc agagaataat
     5701 atgcagcgaa ggagactggt tataaggcag atcaaacagt tcaagttgcg aaaatagcca
     5761 tcccgtcacc ccactcactt caggacggat aaaactaaac ttcagcggat agagcaaaaa
     5821 accgcagcag gccattaccg ttgccagaat aagccggtgg acaagtcggc gctgttcgaa
     5881 tgtcgtgcta caaacgaaca gcgaaaatcc atataaaaga tccagactcc agtaaggaac
     5941 aatagtccag ggaagaaaag ggatcgccgt ttcccaaccg aatacctgac tgggaatatc
     6001 atggctatta ctgtcctgaa ccgcggtgaa ctgattaaga gatccatagg tgaagaagaa
     6061 aaacggggcc agcaacaata accagccagc tccttgtagc aatacgttcc ggcgttcagt
     6121 tatcacgacg caccgccatc gaaaccgtaa aaatgcccca ctcatcaatc cgttgtgtgc
     6181 atttatcaaa tccagcatca tgcacgagca aatccatctc cccttgcgta cgcacgcgca
     6241 ttacccacgg tttaccatct ttatgactgg ttaacacccc ggcaatcagc tccagttgtg
     6301 ggtgccacgg ctgcccggtg tagatgagaa tgccacccgg ttcgatggca ttggcaagac
     6361 ctgcgagtga gttttttacc tgctcatttt cgggaaacag ctcatacagt ccagagacaa
     6421 tcgccagcgt aggccgcgga gttaacgcgc tgagttcctc cgggttaaac gcatcgccct
     6481 gttcaaaacg cacccgccca gacattcccc gttgggcaat catctcttgc ccctgtgcaa
     6541 catttaactc actgtaatca cgtaataaaa tatcgcttac ggcaggctcg tttgccagtg
     6601 catccagtac atagcgccca tgccctgcgg caatgtcaac cacgcggacg gctaaacctt
     6661 tgacgtggag atcggcaacg gcttgtttaa tcagtatttg taaatgagtt ttgcgctggc
     6721 gaataccgcg ccagccaaca ctgttcaggt agattttgtc gattaagcgc ccgaatgcgt
     6781 tactgccttg cggttgattt tgatagacat aatccagcga actgcctgaa tcaaagccag
     6841 tgctcattcc gagatgcagg cccgcagagt gcgtcccgaa caatttcatc gctttacgca
     6901 taaagcgata cgccaaatca accggcgata atggcacggg tccacctgaa aggaggcgcc
     6961 agcgatccgc tgatggtcca gtgcggtctt catgctgata atcaaatttt tgtgacttgt
     7021 tagcatataa acgacgaata aagctttgca ttttttcaaa tgcctgcgcc ctgttctctt
     7081 cacccaacgt gtcgtgataa aagcctggca gcagatgcag ctctttcaga gggctacgta
     7141 atctctgata aaaatcaatc tgcggttggc gatgcaccac atagtcatcg cctgatatca
     7201 gaagttgcgt ggggagcgta atcgccgccg catctctaat aatacgttca gacgttttat
     7261 agagatcgag caagatattg acggcaatcg cccgtgtgat cagcggatca ttattgaaac
     7321 tcgccacccg ttgccgatcg tgggtcaaat agcgtctttt cacataggaa ttaataaaaa
     7381 acagaccacg cagacgatgc catagcgtca gcgcaggacg tgccagcggc acatacaatt
     7441 taaccttaaa ggccggagaa gccagcacca gcccgcgaat tgcaggcgca taatcatgaa
     7501 cccatgtggc aaccagcact gcgccgacgc tttgcgcgat cacaaccacc tcttccagtc
     7561 caacctggct gtcgctggca gcaaaacgga caaattcatc gacatcctgc
//
LOCUS       DAKISA010000159         7574 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.161, whole genome shotgun sequence.
ACCESSION   DAKISA010000159 DAKISA010000000
VERSION     DAKISA010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7574
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.161"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..126
                     /gene="rcbA"
                     /locus_tag="OVW45_003982"
     CDS             <1..126
                     /gene="rcbA"
                     /locus_tag="OVW45_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break reduction protein RcbA"
                     /protein_id="HCV5528312.1"
                     /translation="SKSKLTKSECEKMLSGKKEAGVSREQKVKLINFNCEKLLSS"
     gene            231..455
                     /locus_tag="OVW45_003983"
     CDS             231..455
                     /locus_tag="OVW45_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCV5528313.1"
                     /translation="MYLTLKEWNARQLRPRSPETVRRWVRECKIFPPPIKDGREYLFH
                     ESAKKITPQTTGGLLQRIRNGRTKKKLKHT"
     gene            421..1467
                     /locus_tag="OVW45_003984"
     CDS             421..1467
                     /locus_tag="OVW45_003984"
                     /inference="COORDINATES: protein motif:HMM:NF020572.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HCV5528314.1"
                     /translation="MAGRRRNSNTRDLPPNLYNRGGYYSYRDPRSGREFGIGRDKRIA
                     VNEAIAANMELLSTQHISLLDRIKGNSVTLFCELILNFRNEIERRQLSTNTMKRHNQR
                     LKIISEYFGGVPVKNIGIREVYSFLEIRAAGSKFAIANQYRALLSDIFKTAIASGLAE
                     EDPASATRPFRTEVKRSRLLIDEYLLIRKIADVQNEWFGLCMDLALVTGQREGDLAAM
                     RWEDIRDGRLYVEQQKTGAKIRISLPTTISRLNLTLADVLDNLKKINGKNEKLLGGKT
                     ARTIAAQFRIARDTSGLKWEGDPPPFHEIRSLSGRLHSAEKGGDFTQALLGHRSSSMT
                     DKYRDGRGREWKDI"
     gene            1546..1995
                     /gene="yaiW"
                     /locus_tag="OVW45_003985"
                     /pseudo
     CDS             1546..1995
                     /gene="yaiW"
                     /locus_tag="OVW45_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
     gene            complement(2054..2362)
                     /locus_tag="OVW45_003986"
     CDS             complement(2054..2362)
                     /locus_tag="OVW45_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCV5528315.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            2622..2834
                     /locus_tag="OVW45_003987"
     CDS             2622..2834
                     /locus_tag="OVW45_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCV5528316.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            complement(2858..3952)
                     /gene="ddlA"
                     /locus_tag="OVW45_003988"
     CDS             complement(2858..3952)
                     /gene="ddlA"
                     /locus_tag="OVW45_003988"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCV5528317.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            4030..4233
                     /locus_tag="OVW45_003989"
     CDS             4030..4233
                     /locus_tag="OVW45_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528318.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVREITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFITFIYNKG"
     gene            4415..4675
                     /gene="iraP"
                     /locus_tag="OVW45_003990"
     CDS             4415..4675
                     /gene="iraP"
                     /locus_tag="OVW45_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCV5528319.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            4776..6191
                     /gene="phoA"
                     /locus_tag="OVW45_003991"
     CDS             4776..6191
                     /gene="phoA"
                     /locus_tag="OVW45_003991"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCV5528320.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTSDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKTIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            6310..6630
                     /gene="psiF"
                     /locus_tag="OVW45_003992"
     CDS             6310..6630
                     /gene="psiF"
                     /locus_tag="OVW45_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCV5528321.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            6732..>7574
                     /gene="adrA"
                     /locus_tag="OVW45_003993"
                     /gene_synonym="dgcC"
     CDS             6732..>7574
                     /gene="adrA"
                     /locus_tag="OVW45_003993"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCV5528322.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRG"
BASE COUNT         2125 a         1728 c         1886 g         1835 t
ORIGIN      
        1 tcaaaatcta aactaacgaa atcagaatgc gaaaaaatgc tctcagggaa aaaagaagca
       61 ggcgtttcca gagagcagaa agtaaaactg ataaatttta attgcgagaa acttctgtcc
      121 tcgtgagttg cattatatca aaattagaac ttcatagctg attattaaaa atcaaccacg
      181 cccgccagta ttttgtatat ttactggcgg tcatattaca ggaatatgac atgtatttga
      241 cactcaaaga atggaacgcc cgtcagttac gtccacgaag cccagaaacg gttcgccgct
      301 gggtgcgaga atgtaagatt tttccaccac cgataaagga tgggcgtgaa tatttatttc
      361 atgagtcagc aaaaaaaatt acacctcaaa cgactggcgg gcttttgcag agaatcagaa
      421 atggccggac gaagaagaaa ctcaaacaca cgtgatctac cacccaatct ttacaatcga
      481 ggtggatatt atagttatcg tgacccacgt tcaggaaggg agtttggaat cggaagagac
      541 aaaagaattg cagttaatga ggctattgcg gcaaatatgg aattattatc cacgcaacat
      601 ataagcttac tggatagaat aaaaggtaat agcgtaacat tattctgcga gctgatatta
      661 aatttcagaa atgaaataga gcgtagacaa ttaagcacaa acaccatgaa aagacacaac
      721 cagaggttga aaatcataag tgaatacttt ggtggtgttc cggttaaaaa tataggcata
      781 agggaggtgt acagctttct tgaaatcaga gcagctggga gtaaatttgc tattgctaac
      841 cagtacaggg cgcttttatc agacattttt aaaacagcta tagcttctgg tcttgccgaa
      901 gaagatccag cttctgcaac caggccattc agaactgaag tgaagcgcag tcgactgtta
      961 attgacgagt atctgttaat caggaaaata gcagacgtcc agaacgaatg gttcggcctt
     1021 tgcatggatc ttgcactggt aacaggacag cgcgaaggag atctggcggc tatgagatgg
     1081 gaggatataa gagatggcag gctatacgtg gaacaacaaa aaacaggggc aaaaattcgc
     1141 atttctctcc ccaccacgat ttccagattg aatttaacac ttgccgatgt actggataac
     1201 ttgaaaaaaa taaacggtaa aaacgaaaag ttactcggag ggaagacagc caggacaatc
     1261 gcagcacagt tcaggattgc ccgagacaca tccggattga aatgggaagg tgacccaccg
     1321 ccatttcatg aaatccgaag tttatccggt cgcctgcact cagcagaaaa aggcggtgat
     1381 ttcactcagg cgctccttgg acatcgttca tccagcatga cggataaata ccgtgacgga
     1441 cgagggaggg aatggaaaga catttagaat taatttcgaa tgatttcgaa acaccacttg
     1501 taattaattg attataaatg attaattcct aatgggcatt tgcttcattt actgaattat
     1561 cccgccagct atagcgcacc gatataccgc tttgctgatt ttaacgctgg ctggtacgcc
     1621 agccgtaatg ccgcgtttca gaacgcagtc agtaaggcca gcggcgtgaa gctggcactg
     1681 gacggtgatt taattcgtta cgacagtaaa gaacccggga aaaccgaact ggcaacgcgc
     1741 aaactggcgg gaaaactggg gatgagcgac agcgaaattc gccgtcagtt agagaaaggt
     1801 gacagcttct cttttgagga gacggcgctg tacaagaaag tttatcaact tgccgaagcg
     1861 aaaacgggta aatcactacc ccgcgaaatg ttgcctggca ttcaactgga aagcccgaaa
     1921 atcacccgca acctgactac ggcctggttt gcgaagcgcg tagacgaacg gcgggcgcgt
     1981 tgtatgaaac agtgatcaaa caggaatgtc atgccagata aggcgtttta ggccgtatct
     2041 ggcaacgtaa atcttactgg cggcgatgtc gccagtgcag ccatagcgcc accactgcaa
     2101 aaatgaggca gcctactaaa aacgggatca gcccgaaaat ggtgccgaca cctaaaccaa
     2161 tttccacccg tgggcgacct gtttcctgga cctgcattaa acgctcatag acgccgggcg
     2221 catgcactaa caggttcagc aattgcgccc cagcccaaaa gcaaaacagc acaaacaacg
     2281 cataggcaat gttgccaggc gttgaagagg cattgcgata ttttccgcta aacagcgatt
     2341 ttgacagggt gaaatcagcc ataaaaacct ccggaagtca tctgcttctc ttctggtgtg
     2401 ataccagcgg cttatggcaa gtctgacagg tcatttagat tgtcaatatc agaatcatgg
     2461 taaattgatg ttgggaatat tcccgaagcg tgaatcttcg ttgtgagtta caaaatggca
     2521 accttaagtt aaatttatta acaattacga aaatgttgtc tacaccacag acggttgtta
     2581 cagaatatgc aaggatgttg tttttcgtta acggagctgc catgaatctg cctgtaaaaa
     2641 tccgccgtga ctggcactac tatgcgttcg ccattggcct tatattcatt cttaatggcg
     2701 tggtggggtt actgggattt gaagcaaaag gttggcagac ctatgccgtc ggtctggtga
     2761 cgtgggtgat tagtttctgg ctggcggggt tgattattcg tcgtcgcgat gaagaaactg
     2821 aaaacgccca ataagaatat tatcgggcgt taaaatatta cattgtggtt ttcaatgcgt
     2881 tatccgcagc gtgacgctcc agcgccagct caatcaaacg tgtgatcaga tcggtgtaac
     2941 ccagaccgct ggcttgccac agcttcggat acatactgat gttggtgaag ccaggcagtg
     3001 tgttgatctc gttgatcacc acttcgttct ctggggttaa aaacacgtct acacgcgcca
     3061 tgcctgcgca tcccaacgtc tgataagcct gaacggcaat cgcccggatc ttatcgttga
     3121 tctctggtgc aatggctgcc ggaactacca ctttcgcgcc atcttcgtca atgtacttgg
     3181 tgtcgtaggc atagaaatcg ctggtgagta cgatctcgcc acaggtgctg gcttgcggat
     3241 tgtcgttgcc cagaactgcg cattcgatct cacgaccttt gatcccttgc tcaacgatca
     3301 ctttatgatc gaactcgaac gccagatcga cggcaattgc gtactgttct tcactggtta
     3361 ctttgctgac accaacagaa gagccctgat tagccggttt tacaaacagt ggtaacccca
     3421 gtttagactc cacttcggca aaactgatgt tgtgacgatt agcgcgcgtc agggtaataa
     3481 atggcgcaat gttcagcccg gcatcgcgta gcagacgttt ggtgacatct ttatccatac
     3541 aggctgctga agccagaaca tcagaaccta caaacggtaa attggcgacc cgcagcattc
     3601 cctgcaagga accatcttcg cccagcgtac cgtggacaat cgggaaaatg acatccaccg
     3661 tcggcaacgg ctgaccgttt tgcgcgtcga taagctgatg ctcatgttta cctggcacct
     3721 gcgcaaggct ggtcgccgaa gggcgcaacg caatatgggc aggatcgtct gcatttagca
     3781 gataattgct ggcatcgctg acgtgccatt gcccttgttt atcaatgccc agcagcacaa
     3841 cgtcgaagcg acttttatca atggcatcga caatgttttt tgccgattgc agagacactt
     3901 catgttccgc tgatttacca ccaaaaacga ttcctacccg cagtttttcc atcttaaaaa
     3961 cctatcccgt ctaacacaaa gtgcatacat taccacgaca aaacggggga ttcgcggcct
     4021 tctgaaagat tgttgcaatc ttctgctgac aaagcgtgca acgtactggt gaagaaagtg
     4081 cgttatctca aagatgtgcg cgagatcaca aaaatgatga acgggaagct aatttattcc
     4141 tggcttaaat ggcaatgcgg tgagtttttt tctcttaatt ataagttaac gaagagaata
     4201 tatttcataa cttttattta taataaaggt tgataattaa aagcctatat tttgtgtggg
     4261 taattattta aataagagaa acgtttcgct ggtaatcaaa caaaaaatat ttgcgcaaag
     4321 tatttccttt gtcataaaaa taatacttcc agacactatg aagttgtgaa acataatgtt
     4381 aacttctcca tactttggat aaggaaatac agacatgaaa aatctcattg ctgagttgtt
     4441 atttaagctt gcccaaaaag aagaagagtc gaaagaactg tgtgcgcagg tagaagcttt
     4501 ggagattatc gtcactgcaa tgcttcgcaa tatggcgcaa aatgaccaac agcggttgat
     4561 tgatcaggta gagggggcgc tgtacgaggt aaagcccgat gccagcattc ctgacgacga
     4621 tacggagctg ctgcgcgatt acgtaaagaa gttattgaag catcctcgtc agtaaaaagt
     4681 taatcttttc aacagctgtc ataaagttgt cacggccgag acttatagtc gctttgtttt
     4741 tattttttaa tgtatttgta catggagaaa ataaagtgaa acaaagcact attgcactgg
     4801 cactcttacc gttactgttt acccctgtga caaaagcccg gacaccagaa atgcctgttc
     4861 tggaaaaccg ggctgctcag ggcgatatta ctgcacccgg cggtgctcgc cgcttaacga
     4921 gtgatcagac cgccgctctg cgtgattctc ttagcgataa acctgcaaaa aatattattt
     4981 tgctgattgg cgatgggatg ggggattcgg aaattactgc cgcacgtaat tatgccgaag
     5041 gtgcgggcgg cttttttaaa ggtatcgatg ccttaccgct taccgggcaa tacactcact
     5101 atgcgctgaa taaaaaaact ggcaaaccgg actacgtcac cgactcggct gcatcagcaa
     5161 ccgcctggtc aaccggtgtc aaaacctata acggcgcgct gggcgtcgat attcacgaaa
     5221 aagatcaccc aacgattctg gaaatggcaa aagccgcagg tctggcgacc ggtaacgttt
     5281 ctaccgcaga gttgcaggat gccacgcccg ctgcgctggt ggcacatgtg acttcgcgca
     5341 aatgctacgg tccgagcgcg accagtgaaa aatgtccggg taacgctctg gaaaaaggcg
     5401 gaaaaggatc gattaccgaa cagctgctta acgctcgtgc cgacgttacg cttggcggcg
     5461 gcgcaaaaac ctttgctgaa acggcaaccg ctggtgaatg gcagggaaaa acgctgcgtg
     5521 aacaggcaca ggcgcgtggt tatcagttgg tgaacgatgc tgcctcactg aattcggtga
     5581 cggaagcgaa tcagcaaaaa cccctgcttg gcctgtttgc tgacggcaat atgccagtgc
     5641 gctggctagg gccgaaagca acgtaccacg gcaatatcga taagcccgca gtcacctgta
     5701 cgcctaatcc gcaacgtaat gacagtgtac caaccctggc acagatgacc gacaaaacca
     5761 ttgaattgtt gagtaaaaat gagaaaggat ttttcctgca agttgaaggt gcgtcaatcg
     5821 ataaacagga tcacgctgcg aatccttgtg ggcaaattgg cgagacggtc gatctcgatg
     5881 aagccgtaca acgggcgctg gaattcgcta aaaaggaggg taacacgctg gtcatagtca
     5941 ccgctgatca cgcccacgcc agccaggttg ttgcgccgga taccaaagcg ccgggcctca
     6001 ctcaggcgct aaatactaaa gatggcgcag tgatggtgat gagttacggg aattccgaag
     6061 aggattcaca agaacatacc ggcagtcagt tgcgtattgc ggcgtatggt ccgcatgccg
     6121 ccaatgttgt tggactgacc gaccagaccg atctcttcta caccatgaaa gccgctctgg
     6181 ggctgaaata aaaccgcgcc cggcagtgaa ttttcgctgc cgggtggttt ttttgctgtt
     6241 agcaaccaga cttaatggca gatcacgggc gcaaacgctc atggttaaaa catgaagagg
     6301 gatggtgcta tgaaaataac attactggtt accttgcttt ttggtctggt ttttttaacc
     6361 accgtcggcg ctgccgagag aaccttaacc ccacaacaac agcgtatgac ctcctgtaat
     6421 cagcaggcga cggcgcaggc gttgaaaggg gatgctcgta agacctacat gagtgattgc
     6481 ctgaagaaca gcaagtctgc gcctggtgaa aaaagtttga cgccacagca gcaaaagatg
     6541 cgcgaatgta ataatcaagc aacgcaacaa tctctgaaag gtgatgatcg taataagttt
     6601 atgagtgcct gcctcaagaa agccgcctga tacctgatag tgctaacggg tgagctacga
     6661 aagtggctca cccgaaattt catacttctg cctttagccc cgtctctata atttgggaaa
     6721 attgtttctg aatgttccca aaaataatga atgatgaaaa ctttttcaaa aaagcggcgg
     6781 cgcatgggga ggaacctcct ttaactcctc aaaacgaaca tcagcggtcc gggctgcgct
     6841 tcgcccgtcg cgtcagacta ccccgtgcgg ttggcctggc tggcatgttc ttaccgattg
     6901 cttcaacgct ggtttcgcac ccgccgccgg gctggtggtg gctggtgttg gtcggctggg
     6961 cgttcgtctg gccgcattta gcctggcaga tagcgagcag ggccgtcgat ccgcttagcc
     7021 gggaaattta caacttaaaa accgatgcag tattagcggg aatgtgggta ggcgtaatgg
     7081 gcgtaaacgt gctgccttcc accgctatgt tgatgattat gtgtctgaat ttgatggggg
     7141 caggcggccc ccgtctgttt gtcgcgggtc tggtgttgat ggtggtttcc tgccttgtca
     7201 ccctcgagct tacgggcatt accgtgtcgt tcaatagtgc gccgctggaa tggtggctct
     7261 cccttcccat tattgtcgtt tatcctctgc tgtttggctg ggtcagctac cagacggcaa
     7321 ccaaactggc ggaacataaa cgcaggttgc aggtcatgag tactcgcgac ggcatgacgg
     7381 gcgtgtataa ccgacgtcat tgggaaacta tgttacgcaa tgaatttgat aactgtcggc
     7441 ggcataatcg cgatgcaacg ttactgatta tcgatatcga ccatttcaag agcatcaacg
     7501 atacctgggg tcatgatgtg ggcgatgaag cgattgtggc gcttacccga cagttacaaa
     7561 ttaccctgcg cggt
//
LOCUS       DAKISA010000160         7491 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.162, whole genome shotgun sequence.
ACCESSION   DAKISA010000160 DAKISA010000000
VERSION     DAKISA010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7491
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.162"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(16..522)
                     /gene="mug"
                     /locus_tag="OVW45_003994"
     CDS             complement(16..522)
                     /gene="mug"
                     /locus_tag="OVW45_003994"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HCV5528323.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            647..722
                     /locus_tag="OVW45_003995"
     tRNA            647..722
                     /locus_tag="OVW45_003995"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:680..682,aa:Ile,seq:cat)
     gene            complement(776..1540)
                     /gene="nfeF"
                     /locus_tag="OVW45_003996"
     CDS             complement(776..1540)
                     /gene="nfeF"
                     /locus_tag="OVW45_003996"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HCV5528324.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            1828..2451
                     /gene="nfeR"
                     /locus_tag="OVW45_003997"
                     /gene_synonym="yqjI"
     CDS             1828..2451
                     /gene="nfeR"
                     /locus_tag="OVW45_003997"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCV5528325.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDHAAFDITQLD"
     gene            complement(2558..4078)
                     /gene="aer"
                     /locus_tag="OVW45_003998"
     CDS             complement(2558..4078)
                     /gene="aer"
                     /locus_tag="OVW45_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HCV5528326.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            4496..5875
                     /gene="ygjG"
                     /locus_tag="OVW45_003999"
     CDS             4496..5875
                     /gene="ygjG"
                     /locus_tag="OVW45_003999"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCV5528327.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQADQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(5917..6249)
                     /locus_tag="OVW45_004000"
     CDS             complement(5917..6249)
                     /locus_tag="OVW45_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HCV5528328.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            6468..7451
                     /gene="ebgR"
                     /locus_tag="OVW45_004001"
     CDS             6468..7451
                     /gene="ebgR"
                     /locus_tag="OVW45_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCV5528329.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLFSVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
BASE COUNT         1822 a         2032 c         1869 g         1768 t
ORIGIN      
        1 tttcggtggc gttttttatc gcccacgcac taccagcgcc tggtccagct cgcgatacgc
       61 ttcaaccagt ttctctagtg aaacgcgact taaaccgctg ggatttggca gcacccaaat
      121 ctgcgtcgaa ccaatggtga gcgtttgttt cccccactgt gcaccgcgct ggctgaatcc
      181 ctgttcatat gcttgtttgc ccagaatcgc caacgcctgc ggctgataat cttcaatttt
      241 ttcaatcagc ttacgcccac ctgcgtgcag ctcctgcttt gaaacttcat tggcttgcac
      301 cgttggacgg tctaccagtt tggtgacgcc acaacgataa tccagcagat gctgtgcctc
      361 ctgcggcttc aactgacggt cggtaaaccc ggcctgatat atcaccttcc agaagcgatt
      421 tgctggatga gcaaagggaa aaccagtccc ggcggatgaa agcccagggt tgataccgca
      481 aaacacgacc cgtaaccctg gagccaaaat atcctcaacc atatttactc ctttctaaca
      541 tcaccaggaa aagtataacg gattgaaaat acattgttta taaaaacagc aggcgcacgg
      601 taatggctgg attgcgacac ggagttactt tataatccgc taccatggcc ccttagctca
      661 gtggttagag caggcgactc ataatcgctt ggtcgctggt tcaagtccag caggggccac
      721 cagatatagc aaaggctgac gagaaatcgt cagccttttt ctttttatat atcagttact
      781 ttgcgtgcca gtaagccgct gcacgtaccc gctgtgggtc atactgttcc gcttcaaagc
      841 ggcggcttaa attcttaacg actttacctt cgccggttat ccagatgaag taatcatcgg
      901 cagggatttg catctgcgcc agacgcgcat ctaccgcctg ctcatcatgt gccagccatt
      961 cgatattaaa accatcaagg tgcgcgagat aatcctgaca ggcgttatcc cgcacgctaa
     1021 ccagcgcact aacttgcggt ttaacggcaa gtttgctcaa cgtttccaag cggcggcgca
     1081 gtgcgggcat tccggattca tcgcagacat acagctgata cgcgtaatct tccggcacca
     1141 ccagcgaacc gcgcggacct gccaccgtaa gtttatcgcc cggttgcgcc tgcatcgccc
     1201 agccgctggc gaccccaccg tcgtgaataa agaaatcgat cgccagttca tggcgtagtt
     1261 cgtcatacag cggcgtatag tcacgcgacg gtgggcgtgg tccttccggc cagacgatgc
     1321 cctcttccgt taccgttggc ggcacaaagt gagcgtcagg ttgaggaaag aagagtttgc
     1381 tgtgatcgtc aaagccgcgc gatgtaaaac cgtccagcgc ctcgccgccg aggacaatgc
     1441 gctgaaaacc ggcgctgatg cgctcaacgc gtaacacagt cagttcacgg aagcgcagat
     1501 cattgcgaac gcgctgcggg tagcgggggg agttattcat tgttatcgcc ttcgtgatgg
     1561 taatcagata tatctaaata attttcgtaa atgataatga ttgttaatca tgataaatgc
     1621 aagcgatttg tagaactgat atgtctatag tctgataaga cgaaccgcct cttctcaggc
     1681 atcattactc aacgccggat gcggcgtgaa cgccttatcc ggcctacgtg tgagatgagt
     1741 ccacatttaa aaatcatttt tattcttgca agagtgctca tatcagatat aaattagata
     1801 tatctaatca agcaaaagga ggctgatatg agccatcatc acgaagggtg ttgtaaacat
     1861 gaaggccagc cacgccatga gggctgctgc aaaggtgaga agtcagaaca cgagcactgc
     1921 ggacacggtc accagcatga acacggtcaa tgctgcggtg gtcgccacgg tcgcggcggc
     1981 ggtcgtcggc aacgtttctt tggtcacggt gaattgcgtc tggtgattct ggatattctc
     2041 tcgcgcgatg acagccacgg ttacgaattg attaaagcga ttgagaatct aacccagggg
     2101 aattacaccc caagcccggg cgtcatctac ccaacgctgg attttctgca ggagcagtcg
     2161 ctgattacca tccgcgaaga ggaaggtggt aagaagcaga ttgcgctgac cgaacaaggc
     2221 gcgcagtggc tggaagaaaa ccgcgaacag gtggagatga ttgaagaacg catcaaagcg
     2281 cgttgcgttg gtgctgcgct gcgccagaac ccgcaaatga agcgggcgct ggataatttt
     2341 aaagcggtgc tggatttacg cgtcaaccag agcgatatca ctgatgcaca aataaaaaag
     2401 atcattgcgg tgatcgacca cgccgctttt gatattaccc aactggatta atcaccgatt
     2461 gccggatgcg gcgtacccta cacaccccgc aggcctgata agatgcgcca gcatcgcatc
     2521 aggcattgtg ctccaaccgc cggatccggc ataccgatta atgcagcacc gtcaccgcgt
     2581 cttccagtcg gctggcgcgg tgtttcacca tcgccgacac ctgcgcactc tcttccacca
     2641 gctcagcatt tttctgggtg atcaggttaa gctcatccac tgcacgggtc aggctggaaa
     2701 gcccatcggc ctgttccagc gttgaatggc taatctgggc gatcaactgg gtgacgtttt
     2761 tcacctgtgc cacaatatct tccatcgtcc gtccggcggc gtgtacctgc tgcgaaccgg
     2821 attgcacctt atcagcactg gcatcaatca gcttgcgaat atcgttggca gcattagcgc
     2881 tgcggctggc taaatgacgc acttcccctg ccaccactgc aaaacctttg ccctgttcgc
     2941 cggcacgcgc cgcttccacc gccgcattca gggccagaat attggtctga aacgcaatat
     3001 cgttaatcag cgaagtaatg gtgccaatgc gctgggtact gtcggcgata tcgtccattg
     3061 tcttgatcac cgtggtcatc gcctccccac cctgcactgc cgcattactg gcggtgatag
     3121 aaagtttatc ggcagccgac gccgtggcag agttctgttt caccgacgcc gccatttggt
     3181 tcatggtcgc caccgtttgc tgaacgttat caactgtctg ctgggtatgt tcgttcagtt
     3241 catcggtgcc tttcgccagc gtctcactgc catttctgac actggacacc tggcttgaga
     3301 catcgttaat tagccaacgg cacatcaggc caagctgccc taccgcgcgt aatgtcagcc
     3361 ccagctcatc gctgcgattc aggtgctcaa cactattacg ctctccggtc gccaccttca
     3421 gtgcctgacg ggcgacattt tctatcgggc gcacaatctg ccattcaaaa caagcgcttg
     3481 ccaacaatac cactaacaca cagaggaaat acgtcaccac cggggcagca acaaaccaaa
     3541 gcatggccgc cagcaagata aacatcaggg tcatcactcc acgcgcccgc cagcgaagcg
     3601 gtaatgaagg cagtttaccc agccagcctt tacgcaccac caggccttta tgaatacgct
     3661 tactggtacg tccggcgttc agcgctttgt acagcggctc caccgccgca atctcttcat
     3721 ccgtcgcccg ggtacgaatc gacatatagc cactgatttt tccctcgcgc accatcggta
     3781 ccgcattggc ccgcacccaa taatggtcgc catttttgcg gcgatttttc acgatgccgc
     3841 tccagggctc cccttttttc agggtgaacc acatatccgc aaacgccgct tttggcatat
     3901 ccgggtgacg caccatgttg tgcggctgcc cttgtaactc ttgcaaggta aagccgctca
     3961 cctgcacaaa agtgtcatta gcatgagtga tatagctttg cagatcggta gtggacatca
     4021 gagtggtatc gtccgccagc ggggtatttt gctgggtgac atacggatga gaagacatgt
     4081 tcgcgtcctg tgcaggttat atggttgtta acttcttgtc agagtttatg tcggccccgc
     4141 tgcggttatc tttaaccgat taatttgatt tagatcgcaa tttgcgatta aagcacaaat
     4201 ctaattcttt gatttaaaat actttcactc tgttactata cgaaaacgtt aattatcttg
     4261 cccaaaaatc aggcaattat tgccctgaaa acgtgcattt gcgcagcaat catcaaatcc
     4321 atacccgaca aaaaccgtgc aaaataacaa caaatgttaa cagatagcat taaatattgc
     4381 acaaatgata accgaatttg tgtttatccc gattttcgcg atcgcagccg gagtggcgca
     4441 atccctgcaa tacttaaatc ggtatcatgt gatacgcgag cctccggagc atattttgaa
     4501 caggttacct tcgagcgcat cggctttagc gtgcagcgcc cacgccctga atctcattga
     4561 gaagcgaacg ctggatcatg aggagatgaa agcacttaac cgagaggtga ttgaatactt
     4621 caaagagcat gtcaatccgg ggtttttaga gtatcgcaaa tctgttaccg ccggcgggga
     4681 ttacggagcc gtagagtggc aagcgggaag tttaaatacg cttgtcgaca cccagggaca
     4741 ggagtttatc gactgcctgg gaggttttgg aattttcaac gtggggcacc gtaatccagt
     4801 tgtggtttcc gccgtacaga atcaacttgc gaaacaaccg ctgcacagcc aggagctgct
     4861 cgatccgtta cgggcgatgt tggcgaaaac ccttgctgcg ctaacgcccg gtaaactgaa
     4921 atacagcttc ttctgtaata gcggcaccga gtccgttgaa gcagcgctga agctggcgaa
     4981 agcttaccag tcaccgcgcg gcaagtttac ttttattgcc accagcggcg cgttccacgg
     5041 taaatcactt ggcgcgctgt cggccacggc gaaatcgacc ttccgcaaac cgtttatgcc
     5101 gttactgccg ggcttccgtc atgtgccgtt tggcaatatc gaagccatgc gcacggctct
     5161 taacgagtgc aaaaaaaccg gtgatgatgt ggctgcggtg atcctcgaac cgattcaggg
     5221 tgaaggtggc gtaattctgc cgccgccggg ctatctcacc gccgtacgta agctatgcga
     5281 tgagttcggc gcactgatga tcctcgatga agtacaaacg ggcatggggc gcacgggcaa
     5341 gatgttcgcc tgcgagcatg agaacgtaca gccggatatc ctctgccttg ccaaagcgct
     5401 cggcggcggc gtgatgccga ttggcgcgac catcgccact gaagaggtgt tttcagttct
     5461 gttcgacaac ccattcctgc ataccaccac ctttggcggc aacccgctgg cctgtgcggc
     5521 ggcgctggcg accatcaatg tgttgctgga gcagaactta ccggctcagg ctgatcaaaa
     5581 aggcgatatg ttgctggacg gtttccgtca actggcgcgg gaatatcccg atctggtaca
     5641 ggaagcgcgt ggtaaaggga tgttgatggc gattgagttt gttgataacg aaatcggcta
     5701 taactttgcc agcgagatgt tccgccagcg cgtactggtg gccggaacgc tcaataacgc
     5761 caaaacgatc cgcattgaac cgccactgac actgaccatt gaacagtgtg aactggtgat
     5821 caaagcggcg cgtaaggcgc tggcggccat gcgagtaagt gtcgaagaag cgtaatatca
     5881 aatcggatgg cgatacgacg tcgccatccg atctttttat acgacgcgta cgcccgcagg
     5941 catcatccgc tccggcgtta acagcacgct ttcactgccg tcatcggttt ccgcacacaa
     6001 cagcatgcat tccgacgttt caccacgcat tttcgctttt tgcagattgc ataagaccac
     6061 caccgttttt cccatcagct cctcttcgct gtagtacggc accaggctgg tcacggtttg
     6121 cagtgttttt tcccccacat caacctgtac gatgtacagc ttgtcggcgt tttcatggcg
     6181 tttcacttcc acaatctttc cgacgcgcat ttccagacgt gcaaaatcag cgtaagccac
     6241 ggtttccatt gcttcctccc ttcaagtaaa attttactaa actatagcaa aattttttct
     6301 caatcctgta ggctaaaaat ggagaatgca ggcgtgatca cattcgtaag ccgctgtgtt
     6361 accgttacag cgtcaaagaa acgcgcttta tttactgaaa acaggtgacc cgataagcac
     6421 ttcctctaca atgggggcgc acatcaggga aagtaaaaaa ggtaaacatg gcaacattaa
     6481 aagacatcgc aatcgaagct ggcgtatccc tggcgacagt atccagggtc ttaaatgacg
     6541 atccgacatt gaatgtgaaa gaagagacga aacatcgcat tctcgagatc gccgaaaagc
     6601 tggagtacaa gaccagtagc gcccgtaaac tacagacagg cgcagtcaac caacaccata
     6661 ttctggctat ctacagctac cagcaggagc tggagatcaa cgatccttac tatctggcga
     6721 tccgccacgg cattgaaact cagtgcgaaa agctggcgat cgaactgacc aactgttatg
     6781 aacacaacgg attaccagac attaaaaacg tcaccggtat tttaattgtc ggcaaaccca
     6841 cgcccgccct gcgcgccgct gccagcgcgt tgaccgacaa tatctgtttt atcgactttc
     6901 acgaacccgg cagcggttac gatgcggtgg atatcgatct ggcacgcatc agtaaagaaa
     6961 tcatcgactt ctatatcaac cagggcgtta atcgtattgg ttttattggc ggtgaagatg
     7021 agcctggcaa ggcggatatt cgtgaggtcg cctttgcgga atatggccga ctgaaacaag
     7081 tggtacgcga agaggatatc tggcgcggcg gtttttccag ttcgtcgggt tatgaactgg
     7141 caaaacaaat gctggcgcgg gaagactatc cgaaggcact gtttgttgct tccgattcca
     7201 ttgctatcgg cgtactgcgg gcaattcatg aacgtggcct gaacatccca caggatattt
     7261 cgcttttcag cgttaacgat atccccaccg cgcgatttac ctttccgccg ctctccaccg
     7321 tgcgcatcca ttccgagatg atgggaagtc agggcgttaa cctggtgtat gaaaaagccc
     7381 gcgatggtcg cgcgctgccg ctgctagtct tcgttcccag caaattaaaa ctgcgcggca
     7441 cgacccgtta aatcccctta cacactgtcc ggcaatcatt tttgccggac a
//
LOCUS       DAKISA010000161         7473 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.163, whole genome shotgun sequence.
ACCESSION   DAKISA010000161 DAKISA010000000
VERSION     DAKISA010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7473
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.163"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..571
                     /locus_tag="OVW45_004002"
     CDS             <1..571
                     /locus_tag="OVW45_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCV5528330.1"
                     /translation="ADNAAGTAEQMFTVAFSSAGNALATFCTTGKLNFKSFTSSLLSD
                     MARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVNRPTFFAFAKGAG
                     VMGEAGPEAILPLRRGADGKLGGVADTGGSGMVMFAPQYNIEINNDGTNGQIGPAALK
                     VVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            571..900
                     /locus_tag="OVW45_004003"
     CDS             571..900
                     /locus_tag="OVW45_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCV5528331.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPFLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            910..1608
                     /locus_tag="OVW45_004004"
     CDS             910..1608
                     /locus_tag="OVW45_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCV5528332.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            1613..2356
                     /locus_tag="OVW45_004005"
     CDS             1613..2356
                     /locus_tag="OVW45_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCV5528333.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRNGQNLYLDNMEVT
                     GFYRVALTEAQPGDVLLCCFGSSVPNHAAIYCGDGGLLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIYNDLVAASTFV"
     gene            2293..2895
                     /locus_tag="OVW45_004006"
     CDS             2293..2895
                     /locus_tag="OVW45_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCV5528334.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            2956..6435
                     /locus_tag="OVW45_004007"
     CDS             2956..6435
                     /locus_tag="OVW45_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCV5528335.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVTDGFGGTEPRITCNSYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQMLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITAVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAIGRASDDAEGYLNFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKCLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKATAGITIS"
     gene            6503..7102
                     /locus_tag="OVW45_004008"
     CDS             6503..7102
                     /locus_tag="OVW45_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCV5528336.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLQTHTDMPG
                     SDDLKGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDGGRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            7166..>7473
                     /locus_tag="OVW45_004009"
     CDS             7166..>7473
                     /locus_tag="OVW45_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCV5528337.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     "
BASE COUNT         1688 a         1780 c         2382 g         1623 t
ORIGIN      
        1 tgcggataat gcggcgggaa cggcagaaca gatgtttaca gtggcattca gcagtgccgg
       61 taatgcactg gcgacattct gtaccaccgg taagctgaat tttaaatcct tcacctcttc
      121 cctgttgtca gatatggcca gaattatggc acagatggcc atgatgcagg cggtaaaggg
      181 cgtcggttct ttattcggct tcacgactaa tgctgatggc ggtgtttacc agtctgctga
      241 tttgagtcgc tacagtggca cggtggttaa ccgtccgacg ttttttgctt ttgcaaaagg
      301 cgcgggtgtg atgggggaag cgggacctga agccattctg ccactgcgtc gcggtgctga
      361 tggtaagctg gggggtgtgg cggatactgg tggttcaggc atggtgatgt ttgccccgca
      421 gtacaacatt gagatcaata acgatggcac gaacgggcag ataggtccgg ctgccctgaa
      481 ggtggtttat gacctcggga aaaaagcggc cgcggacttt atgcaacagc aggcccgtga
      541 tggtggccgg ttaagtggag cataccggta atggagacgt ttcactggaa agtgcgcccg
      601 gatatgaatg tggtatcaga gccgaaagtg gtgacagtga agctgggcga tggttatgaa
      661 cagcgtcgtg cggcgggact gaataaccag ttgtcgactt acagcgtgac gatacgtgtt
      721 cgtaaaggtg aacacccatt tttaaaagcc tttctggaac ggcacggtgg cgtccgcgca
      781 tttcagtgga cgccacctta tgactggaag ccgatcaggg tggtttgtcg taaatggtcg
      841 gcaagcgtgg gggcgttgtg ggtaaccata acggcagatt ttgaacaggt cgtggcatag
      901 gaggctctga tgcaggatat tccacaggaa acacatcatg agacgacacg cctcactcag
      961 tcagcccagg tggtgctctg ggaaatcgat ctgacagagg tcggtggtga acgttatttt
     1021 ttctgtaatg agcagaacga aaaaggtgag ccggttacct ggcaggggcg gcagtatcag
     1081 gcatacccca ttcaggggac gggatttgaa ctgaacggca agggcagtgc tgcccgtccg
     1141 acactgacgg tttctaacct gcacggaatg gtcaccggga tggcggaaga cctgcagagt
     1201 ctggtcggcg gaacggtggt caggcgtaag gtttacgccc gttttctgga tgcggtgaac
     1261 ttcgtcaacg gaaacagcga cgccgatccg gaacaggagg tgatcagccg ctggcgcatc
     1321 gagcagtgca gcgaactgag cgcggtcagt gcctcttttg tgctggccac accaacggag
     1381 acggatggcg cggttttccc ggggcgtatc atgctggcga atacctgtat gtggacttac
     1441 cgttctgatg agtgtggtta cacgggcagg gcagtggctg acgagttcga caaaccaacg
     1501 acggatatcc ggaaggacaa atgcagcaag tgtatgcgcg ggtgtgagtt gcgcaacaat
     1561 accggtaatt tcggcggttt cctttccatc aataaacttt ctcagtaaat ccatgacaca
     1621 gacagaatca gcgattctgg cgcacgcccg gcgatgtgcg ccagcggagt cgtgtggctt
     1681 cgtggtgaga acgccggagg gagacaggta tcttcccagc gagaatatct ccggtgagcc
     1741 ggaggaacgg ttccggatgg ctccggagga ctggctgcgg gcacaaatgc agggtgagat
     1801 tgtggcgctg gtccacagtc atcccggtgg tctgccctgg ctgagtgagg ctgaccggcg
     1861 gctgcaggtg cagagtgatt tgccgtggtg gctggtctgc cggggggcga ttcacaagtt
     1921 ccgctgtgtg ccgcatctca ccgggcggcg ctttgagcac ggggtgacgg actgttacac
     1981 gctgttccgg gatgcttatc atctggcagg gattgagatg ccggattttc atcgcgggga
     2041 tgactggtgg cgtaacggtc agaatctcta tcttgacaat atggaggtga cgggttttta
     2101 ccgtgtcgca ctgacagagg cacagccggg cgatgtgctg ctgtgctgtt ttggttcatc
     2161 ggtgccgaat catgccgcca tttactgcgg tgacggcggg ctgctgcacc atattcctga
     2221 acaactgagc aaacgagaga ggtacaccga caaatggcag cgacgcacac actccctctg
     2281 gcgtcaccgg gcatggcgcg catctgcctt tacggggatt tacaacgatt tggtcgccgc
     2341 atcgaccttc gtgtgaaaac gggggctgaa gccatccggg cactggccac acagctcccg
     2401 gcgtttcgtc agaaactgag tgacggctgg tatcaggtgc gcattgccgg gcgtgatgca
     2461 ggtgaaaccg aattgtctgc ccgtcttaat gagccgctgg caaatggtgc agtgatccac
     2521 atagtaccgc gtctggtggg agctaaaagt ggcggtgtgt ttcaggcggt gctgggggcg
     2581 gctgttatgg cggttgctat atggatgccg ggggtaggaa ttatggcgag taatctgctg
     2641 ttttctctcg gtgccagtat gacgcttggc ggtgttgcac agatgctggc accgaaagcc
     2701 agaactcccc gtacacagac aacggataac ggtaagcaga acacgtattt ctcgtcactg
     2761 gataacatgg ttgcccaggg caatgttctg cccgttctgt acggtgaaat gcgcgtgggg
     2821 tcacgtgtgg tttctcagga gatcagcacg gcagacgaag gggatggtgg tcaggttgtg
     2881 gtgattggtc gctgatgcaa aatgttttat gtgaaaccgc ctgcgggcgg ttttgtcgtt
     2941 tatggagcgt gaggaatggg taaaggcagc agtaaggggc ataccccgcg cgaagcgaag
     3001 gacaacctga aatccacgca gttgctgagt gtgattgatg ccatcagcga agggccggtt
     3061 gacggtccgg tggatggatt aaaaagcgtg ctgctgaacg gtacgccggt cctggacagc
     3121 gaggggaaga caaacttttc cggtgttacg gtggtgttcc gtgcaggtga gcaggagcag
     3181 acaccgccgg agggttttga atcctccggc tccgagacgg tgctgggtac ggaagtgaaa
     3241 tatgacacgc cgatcacccg gaccatcacg tcggcaaaca tcgaccgtct gcgcctgacc
     3301 ttcggtgtgc aggcactggt ggaaaccacc tcaaagggtg accggaatcc gtcggaagtc
     3361 cgcctgctgg ttcagataca gcgtaacggt ggctgggtga cggaaaaaga catcaccatt
     3421 aagggcaaaa ccacttcgca gtatctggcc tcggtggtgg tgggtaacct gccgccgcgc
     3481 ccgtttaata tccggatgcg caggatgaca ccggacagca ccacagacca gctgcagaac
     3541 aaaacgctct ggtcgtcgta caccgaaatc atcgatgtga aacagtgcta cccgaacacg
     3601 gcgctggtcg gcgtgcaggt ggattcagag cagttcggta accagcaggt gagtcgcaat
     3661 tatcatcttc gcgggcgcat tctgcaggtg ccgtcgaact ataacccgca gacgcggcaa
     3721 tacagcggta tctgggacgg aacgcttaag ccagcataca gcaacaacat ggcctggtgt
     3781 ctgtgggaca tgctcactca tccgcgctac ggcatgggga aacgtcttgg tgcggcagat
     3841 gtggacaaat gggcgctgta tgtcatcggc cagtactgcg accagtcagt gacggacgga
     3901 tttggcggca cggagccgcg catcacctgt aactcttacc tgaccacaca gcgtaaggcg
     3961 tgggatgttc tcagtgattt ctgctcggcg atgcgctgta tgccggtatg gaacggacag
     4021 atgctgacgt tcgtgcagga ccgaccgtcg gataaggtgt ggacctataa ccgcagtaat
     4081 gtggtgatgc cggacgatgg cgcgccgttc cgctacagct tcagcgccct gaaggaccgc
     4141 cataatgccg ttgaggtgaa ctggattgac ccagataacg gctgggagac ggcgacagag
     4201 cttgtggagg atacgcaggc cattgcccgt tacggtcgta atgtcacgaa gatggatgcc
     4261 tttggctgta ccagccgggg gcaggcacac cgagccgggc tgtggctgat taaaacggaa
     4321 ctgctggaaa cgcagaccgt ggacttcagc gtgggcgcag aagggcttcg ccatgtaccg
     4381 ggtgatgtca ttgaaatctg cgatgatgat tatgccggta tcagcaccgg tggtcgcgta
     4441 ctggcggtga acagccagac ccggacgctg acgctcgacc gtgaaatcac gctgccgtcc
     4501 tccggtacca cgctgataag cctggttgac ggaagtggca atccggtcag cgtggaggtc
     4561 cagtccgtca ccgacggcgt gaaggtgaaa gtgagccggg ttcctgacgg cgttgcagaa
     4621 tacagcgtgt gggggctgaa gctgccgacg ctgcgccagc gtctgttccg ctgcgtgagt
     4681 atccgtgaga acgacgacgg cacgtatgcc atcaccgccg tgcagcatgt gccggaaaaa
     4741 gaggccatcg tggataacgg ggcacacttt gacggtgacc ggcgcggcac ggtgaatggt
     4801 gtcacgccgc cagcggtgca gcatctgacc gccgaagtca ccgcagacag cggggaatat
     4861 caggtgctgg cgcgatggga cacgccgaag gtggtgaagg gtgtgagctt cctgcttcgc
     4921 ctgaccgtgg cagcggacga cggcagtgag cggctggtca gcacagccag gacgacggaa
     4981 accacatacc gcttcaggca gctggcgctg gggaattaca gtctgacagt ccgggcggta
     5041 aatgcccggg ggcagcaggg cgatccggcg tcggtatcgt tccggattgc ggcaccggca
     5101 gcgcctgtca ctattgaact gataccgggg tattttcaga taacggcggt cccgaaactg
     5161 gctgtatatg acccgacggt gcagtttgag ttctggtttt cggaaaagcg gattgcggat
     5221 atcaggcagg ttgaaaccag cgcccgctat cttggcacgg cgctgtactg gatagccgcc
     5281 agtatcaata tcaggccggg ccatgattat tatttttacg ttcgcagtgt gaacaccgtt
     5341 ggcaaatcgg catttgtgga ggccatcggt cgggcgagcg atgatgcaga aggttatctg
     5401 aatttttata aagggttgat caataaaacg catctcggca aggagttgtg gacgcagatt
     5461 gataacggtc agcttgcgcc ggacctgact gaaatcagga cgtccattac gaatgtcagc
     5521 aatgaaatca cgcaaaccgt caataaaaaa ctggaaaatc agagtgctgc aatccagcag
     5581 atacagaaag ttcaggttga tacaaataat aacctgaaca gcatgtgggc tgtgaagctg
     5641 caacagatga aggacggacg cctttatatt gcgggtatcg gtgccggtat tgagaatacg
     5701 ccagcaggaa tgcagagtca ggtgctgctg gcggcagaca ggattgcgat gattaatcct
     5761 gcgaatggca acacaaagcc gatgtttgtt ggtcagggcg atcagatatt catgaacgac
     5821 gtgttcctga aatgcctgac ggctccgacc attaccagcg gcggtaatcc tccggcattt
     5881 tccctgacac ctggcggacg gctgacggcg aaaaatgccg atatcagcgg taacgtgaac
     5941 gcgaactccg ggacgctcaa caacgtcacg attaataaga actgtcgggt tctgggaaaa
     6001 ttgtccgcga accagattga aggcgatctc gttaaaacag tgggcaaacc tttcccacgg
     6061 gactcccggg caccggagag gtggccatca gggaccatta ccgtcagggt ttatgacgat
     6121 cagccgtttg atcggcaaat tgttattccc gcggtggcgt ttcgcggtgc taaacatgag
     6181 cggaagaata acaatattta ttcgtcatgc cgcctgatag tgaagaaaaa cggtgctgaa
     6241 atttataacc gaacgaccct ggataatacg ctgatatata cgggtgttat tgatatgcct
     6301 gccggtcacg gtcacatgac gctggagttt tctgtatcgg catggctggt aaatggctgg
     6361 tatcccacag caagtatcag cgatttgctg gttgtggtga tgaagaaagc cactgcaggc
     6421 atcacgatta gctgaatttt ataacccaga tacgggcacc agaaatggtg ccttttttat
     6481 tgcagaaaag cgagaggtaa ttatgcgtaa agtttgtgca gccattttgt ccgcagccat
     6541 ctgtctggcc gtatccggtg cgcctgcatg ggcgtctgaa catcagtcca cgctgagcgc
     6601 cgggtatctt cagacccata ctgatatgcc aggcagcgat gacctgaagg gcattaacgt
     6661 gaaataccgt tatgagttta cggacacgct ggggctgatt acgtcattca gttatgccaa
     6721 cgctgaagat gagcaaaaaa cgcattacag cgatacccgc tggcatgagg attccgtgcg
     6781 taaccgctgg ttcagcgtga tggcggggcc gtctgtgcgc gtgaatgaat ggttcagcgc
     6841 gtatgcgatg gcgggcgtgg cttacagccg tgtgtcgacc ttctccgggg attatctccg
     6901 cgtaactgac aacaagggga aaacgcacga tgtgctgacc gggagtgatg gcggtcgcca
     6961 cagcaacacg tctctggcgt ggggggctgg cgtgcagttt aacccgaccg aatccgtggc
     7021 cattgacctt gcttatgaag gttccggcag tggtgactgg cgcactgacg gtttcatcgt
     7081 gggtgtcggt tataagttct gattagccag gtaacacagt gttatgactg cccgctggtt
     7141 caggcgggct ttttgtgggg tgaatatggc agtaaagatt tcaggtgtac tgaaagatgg
     7201 ggcgggtaaa cctgtcgtaa attgtgcgat tgaactgcgg gccagaagaa ccagtccgac
     7261 cgttgtggca cacgttgttg ccacttgcgt gacggacaat aacggtgctt atgtgattga
     7321 ggctgagccg gggtattacg aggttgcgct tcactgtaac ggctggcagc caacccgtgt
     7381 cggggatatt gatgtggcac cgactgatgc accggggaca ctgaacgcgt ttctgaatgc
     7441 accaaaggat ggtgatttac gtccggaggt gat
//
LOCUS       DAKISA010000162         7381 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.164, whole genome shotgun sequence.
ACCESSION   DAKISA010000162 DAKISA010000000
VERSION     DAKISA010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7381
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.164"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(34..121)
                     /locus_tag="OVW45_004010"
     tRNA            complement(34..121)
                     /locus_tag="OVW45_004010"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(85..87),aa:Ser,seq:gga)
     gene            355..1293
                     /gene="ghrA"
                     /locus_tag="OVW45_004011"
     CDS             355..1293
                     /gene="ghrA"
                     /locus_tag="OVW45_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HCV5528338.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            1348..2085
                     /gene="ycdX"
                     /locus_tag="OVW45_004012"
     CDS             1348..2085
                     /gene="ycdX"
                     /locus_tag="OVW45_004012"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCV5528339.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQTKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            2109..2663
                     /gene="ycdY"
                     /locus_tag="OVW45_004013"
     CDS             2109..2663
                     /gene="ycdY"
                     /locus_tag="OVW45_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCV5528340.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDVTEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            2765..3256
                     /locus_tag="OVW45_004014"
     CDS             2765..3256
                     /locus_tag="OVW45_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCV5528341.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3321..4154)
                     /gene="csgG"
                     /locus_tag="OVW45_004015"
     CDS             complement(3321..4154)
                     /gene="csgG"
                     /locus_tag="OVW45_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HCV5528342.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(4181..4597)
                     /gene="csgF"
                     /locus_tag="OVW45_004016"
     CDS             complement(4181..4597)
                     /gene="csgF"
                     /locus_tag="OVW45_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCV5528343.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(4622..5011)
                     /gene="csgE"
                     /locus_tag="OVW45_004017"
     CDS             complement(4622..5011)
                     /gene="csgE"
                     /locus_tag="OVW45_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCV5528344.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            complement(5016..5666)
                     /gene="csgD"
                     /locus_tag="OVW45_004018"
     CDS             complement(5016..5666)
                     /gene="csgD"
                     /locus_tag="OVW45_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCV5528345.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            6419..6874
                     /gene="csgB"
                     /locus_tag="OVW45_004019"
     CDS             6419..6874
                     /gene="csgB"
                     /locus_tag="OVW45_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCV5528346.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSTQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            6915..7370
                     /gene="csgA"
                     /locus_tag="OVW45_004020"
     CDS             6915..7370
                     /gene="csgA"
                     /locus_tag="OVW45_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HCV5528347.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSVLALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
BASE COUNT         1947 a         1630 c         1782 g         2022 t
ORIGIN      
        1 gcccgtactt tcgtacgagc tcttctttaa atatggcggt gaggggggga ttcgaacccc
       61 cgatacgttg ccgtatacac actttccagg cgtgctcctt cagccactcg gacacctcac
      121 caaattgtcg ttcctgtctt gctggaacgg gcgctaattt agggaaatca tgacctgagg
      181 tcaacaaact ttttgaaaaa atcgcgcgtt tattcaaact tcaatcaatg tgtggtttta
      241 ataagcgaaa tctgcttttt ttgccaccga ccacggattt gttatgctgg tggcctttgt
      301 agatcataac gataagtgcg aataaatttc gcacaacgct tttcgggagt cagtatggat
      361 atcatctttt atcacccaac gttcgatacc caatggtgga ttgaggcact gcgcaaagct
      421 attcctcagg caagagtcag agcatggaaa agcggagata atgactctgc tgattatgct
      481 ttagtctggc atcctcctgt tgaaatgctg gcagggcgcg atcttaaagc ggtgttcgca
      541 ctcggggccg gtgttgattc tattttgagc aagctacagg cacaccctga aatgctgaac
      601 ccttctgttc cactttttcg cctggaagat accggtatgg gcgagcaaat gcaggaatat
      661 gctgtcagtc aggtgctgca ttggtttcga cgttttgacg attatcgcat ccagcaaaat
      721 agttcgcatt ggcaaccgct gcctgaatat catcgggaag attttaccat cggcattttg
      781 ggcgcaggcg tactgggcag taaagttgct cagagtctgc aaacctggcg ctttccgctg
      841 cgttgctgga gtcgaacccg taaatcgtgg cctggcgtgc aaagctttgc cggacgggaa
      901 gaactgtctg catttctgag ccaatgtcgg gtattgatta atttgttacc gaatacccct
      961 gaaaccgtcg gcattattaa tcagcaatta ctcgaaaaat taccggatgg cgcgtatctc
     1021 ctcaacctgg cgcgtggtgt tcatgttgtg gaagatgacc tgctcgcggc gctggatagc
     1081 ggcaaagtta aaggcgcaat gttggatgtt tttaatcgtg aacccttacc gcctgaaagt
     1141 ccgctctggc aacatccacg cgtgacgata acaccacatg tcgccgcgat tacccgtccc
     1201 gctgaagctg tggagtacat ttctcgcacc attgcccagc tcgaaaaagg ggagagggtc
     1261 tgcgggcaag tcgaccgcgc acgcggctac taataaagca tcaggattcc tgctatcctt
     1321 ggcgggaatt gaatacagga gagagttatg tatcccgtcg accttcatat gcataccgtt
     1381 gccagcacac atgcatatag cacattgagt gattacattg cccagaccaa acaaaagggc
     1441 attaaacttt ttgcgatcac cgatcatggc ccggatatgg aagatgctcc gcatcactgg
     1501 cacttcatta acatgcgtat ctggccgcga gtggttgatg gggtagggat cctgcgcggc
     1561 atcgaagcta acattaaaaa tgttgatggt gaaattgatt gcagcggtaa aatgtttgac
     1621 tcgctggatc tgattattgc cggttttcat gagccggttt ttgcaccaca tgacaaagcg
     1681 accaatacac aagcgatgat cgccactatc gccagcggca atgtgcatat aataagccat
     1741 cccggaaatc ccaaatatga aatagatgtg aaagccgttg ctgaagcagc cgcgaaacat
     1801 caggtggcgc tggaaatcaa taattcttca tttttacact cacgtaaggg cagtgaagac
     1861 aactgtcgtg cggtagccgc agcggtacgt gatgctggag gttgggtggc attaggctcg
     1921 gattctcaca ccgcgtttac catgggggaa tttgaagagt gtcttaaaat cctcgacgcg
     1981 gtagattttc cgccagagcg cattttgaat gtttctccgc gccgcttact gaacttcctt
     2041 gaatctcgcg gtatggcacc gattgcggaa tttgcagacc tttaattact agtaatggaa
     2101 atatattaat gaacgagttt tctatcctct gtcgtgtgct gggttcgctc tattaccgcc
     2161 aaccgcaaga tcctttactg gtgccgctgt ttaccctgat tcgtgagggg aaactggctg
     2221 cgaactggcc actggagcag gatgagttac tgacgcgttt acagaaaagc tgtgatatgg
     2281 cgcaagtctc tgccgattac aatgcgttgt ttatcggcga tgaatgtgcc gtgccgccat
     2341 atcgtagcgc atgggttgag gacgtaacgg aagcggaagt gcgcgctttt ctttccgaac
     2401 gagggatgcc actggcggat acgccagccg atcacatcgg cacattgctg cttgcggctt
     2461 cctggctgga agatcagtca acggaagatg agagcgaagc actggaaaca ctgttcagtg
     2521 agtatctgtt accctggtgt ggtgcgttcc tcggcaaagt ggaggcccat gcaaccacgc
     2581 ctttctggcg caccatggca ccgctaaccc gtgatgccat tagtgcaatg tgggacgagc
     2641 tggaagaaga ttctgaagag taaatgtgat catcgtcact cataactgca acagataatt
     2701 tgttgttgca taaaatgtgt gctcgatctc attcatggcc gcgttttctg ctatcatgcg
     2761 cggcatgaac atacttctct ctattgcaat cacaacaggc attctctccg gtatctgggg
     2821 atgggtggct gtttccctgg gcttactaag ctgggcgggc ttcttgggct gtacggccta
     2881 ctttgcctgc ccgcaaggtg ggctgaaagg gctggcgatc tccgctgcaa ccctgcttag
     2941 tggcgtggtg tgggcgatgg tcattattta cggtagtgca ctggcaccac atctggaaat
     3001 tctcggttat gtcataaccg gtattgtggc gtttctgatg tgtattcagg ccaaacagct
     3061 gctgctttca tttgttccgg ggacgtttat aggcgcatgc gcgacgtttg ccgggcaggg
     3121 tgactggaaa ctggtgttac cttcgctggc gcttgggctg gtatttggtt acgcaatgaa
     3181 aaacagcggc ctgtggctgg cggcgcgtag tgcaaagacg gctcaccgtg agcaggaaat
     3241 caaaaataaa gcgtgagggg cactcacgct ttcgcttaaa cagtaaaatg ccggattatt
     3301 aattccggca tttttatctg tcaggattct ggtggaaccg acatatggcg gtatttaacc
     3361 agaatgtcat tctgccgttc tgctttgttc tgcaaatccc acagaccacg gtcgatacca
     3421 tcattaatca ggaaaatgac ccctgtttcg atagccgaca tcagacacag cataacgggt
     3481 tcgtttgagg tgtaacccac ttccccttcc agcaagcgct ggtagtcaat aaagcggaaa
     3541 accccggcct gaacttcata ggaaagtatc gtcttactgg tgttcaccga ggagaggatc
     3601 tcaccggtac tcacattgac gacacgcagg ttcacggcaa tctgatcgag ctggtattgc
     3661 gtgtctgcac cgatcccaaa atatcttgcc ccaacaccgc ccgatttaac gttgctttca
     3721 taaccgataa tcgaaccttc aaccatgata tttgccgccg ttaaagattg cagcgggatt
     3781 cggttattaa tggcaactgt gccgttttct tgtgccgcac gaataatctt gcgctcgtta
     3841 agcaggtttt gtaaaccctg acgctccagc ggtataaacc agcgagaatc tttcaatgcc
     3901 gtgaccagca ttgccgtggc gctttgcgga acagcagtgg agaagttact tgccgggtag
     3961 ggtttaaatt gcccggtttc gtcctgaatg ttgtataccg aaacaaagat cttacccgtc
     4021 ggcgctggca gatgggtcaa atctttgtag ctctgagctc gaggcattaa tgtcggtctg
     4081 gcggcttctt taggcggggc ggttaagcat ccgctcagta acatgacggc aaccaaaaga
     4141 aataagcgct gcatgattat tttccttatg aagctggggc ttaaaaatcg gttgagttat
     4201 tttgtaaacc cgaaacctgg atggtcgagg tttgtccggt tttacgatct gtcacgttca
     4261 actgcaattg accatcgcgg ttagcaatat caacaatata atcgttggtc accatgcggc
     4321 ccggtttacc ggtattaata ttcgacagta gcccacctaa aatttgtgac tggatggcct
     4381 gagtaaagtt atctaacgct gagggtgttt caataccaaa gtcatcgtta tagctcggat
     4441 ctttataaga gttttgggcc tgagcgctat ttaataaaaa agcgccatta tttgggttac
     4501 caccaaagtt tggattacgg aactggaaag tcatggttcc agcccaactt aatggcgaaa
     4561 taagcatgag tagaactact gcatgtttga cacgcattgc agcctccgaa caatttttta
     4621 tttagaattc atcatgcgcc aaatcgcccg tacttaataa cgcctgattt atctggcgac
     4681 gatttagtgc ttcttcagtt tgaatcagtg caaagacgac agttttctcg aagtctcttt
     4741 tcaacggaaa taaaaaagtc tggaaaataa cgtcctgatt gaccgttata gtgatccagc
     4801 ttccccatcg tgcactgggc ctttcattaa tcgttaagtt acccgtatag tcactttccc
     4861 atttatcact aaaggctcgg taaaaatcat ggccaataga tgaaacagta tggtcagtta
     4921 gcaatcccgg gacttctacc tcaacggcgt gaagattccc tgcggcgaac agaaattctg
     4981 ccgccacaat ccagcgtaaa taacgtttca tggctttatc gcctgaggtt atcgtttgcc
     5041 caggaaaccg cttgtgtccg gttttttacg gctatcttct tgaaaagatt ataaagatgc
     5101 gttttaaccg tattttcgct gatgaacaac gaacgagcga tctcgttatt agacgcgccg
     5161 atacgcagtt tattcaggat ctctttttcc cgatgagtaa ggagggctga ttccgtgctg
     5221 ttataacgat agttacctga atgcgtaatc aagtagctgg caagcttttg cgtaaagtag
     5281 cattcgccgc gcaggacgcc ttgcaaccca ttgacaacac gttcttgatc ctccatggca
     5341 taaaaaacgc cgttgatatg aggccagttt tcaatgtcgc ggtacgggta atcttcaggc
     5401 gtatttagca acaatatttt gatattgttg tttttcctgc tcaaagtatc ctgccaataa
     5461 tggataagct ttttatccgc ttccatcata tccagaagaa taatagagcc tgaagatata
     5521 tcgtccagag aacgttgaat attatgtaat tttcctgtaa ttgccagcga ttgtttaagg
     5581 tgctgcaaga gagccgtcgc ctgcaaagaa ggtttagtga tcaacaataa tgtatgacca
     5641 tgaatactat ggacttcatt aaacatgatg aaaccccgct ttttttattg atcgcacacc
     5701 tgacagctgc ctctaaaata gaagcaccag aagtactgac agatgttgca ctgctgtgtg
     5761 tagtaataaa tcagccctaa atgggtaaaa tataaaacta atggattaca tctgatttca
     5821 atctagccat tacaaatctt aaatcaagtg ttaaacatgt aactaaatgc aactcgttat
     5881 attaaaatgt taatcttaag gttttattaa gtttagaaat gatagaaaag ttgtacattt
     5941 ggtttttatt gcacaatttt aaaaaatcat acaaatggtg ataacttact aataatgcat
     6001 ataaaaaata tttcggtgta gtcctttcgt catgtaaaaa gttgttgttt tttctccaca
     6061 cctccgtgga caaattttta ctccaaaaag acgaggtttg tcacggcttg tgcgcaagac
     6121 atatcgcagc aatcagcgat gggcaagaag aatgactgtc tggtgatttt tgatagcgga
     6181 aacggagatt taaaagaaaa caaaatattt tttgcgtaga taacagcgta tttacgtggc
     6241 ttttaatact ttggtatgaa caaaaaaaga aaaatacaac gcgcgggtga gttattaaaa
     6301 atatttccgc agacatactt tccatcgtaa cgcagcgtta acaaaataca ggttgcgtta
     6361 acaaccaagt tgaaatgatt taatttctta aatgtacgac caggtccagg gtgacaacat
     6421 gaaaaacaaa ttgttattta tgatgttaac aatactgggt gcgcctggga ttgcagccgc
     6481 agcaggttat gatttagcta attcagaata taacttcgcg gtaaatgaat tgagtaagtc
     6541 ttcatttaat caggcagcca taattggtca agctgggact aataatagta ctcagttacg
     6601 gcagggaggc tcaaaacttt tggcggttgt tgcgcaagaa ggtagtagca accgggcaaa
     6661 gattgaccag acaggagatt ataaccttgc atatattgat caggcgggca gtgccaacga
     6721 tgccagtatt tcgcaaggtg cttatggtaa tactgcgatg attatccaga aaggttctgg
     6781 taataaagca aatattacac agtatggtac tcaaaaaacg gcaattgtag tgcagagaca
     6841 gtcgcaaatg gctattcgcg tgacacaacg ttaatttcca ttcgactttt aaatcaatcc
     6901 gatgggggtt ttacatgaaa cttttaaaag tagcagcaat tgcagcaatc gtattctccg
     6961 gtagcgctct ggcaggtgtt gttcctcagt acggcggcgg cggtaaccac ggtggtggcg
     7021 gtaataatag cggcccaaat tctgagctga acatttacca gtacggtggc ggtaactctg
     7081 tacttgctct gcaaactgat gcccgtaact ctgacttgac tattacccag catggcggcg
     7141 gtaatggtgc agatgttggt cagggctcgg atgacagctc aatcgatctg acccaacgtg
     7201 gcttcggtaa cagcgctact cttgatcagt ggaacggcaa aaattctgaa atgacggtta
     7261 aacagttcgg tggtggcaac ggtgctgcag ttgaccagac tgcatctaac tcctccgtca
     7321 acgtgactca ggttggcttt ggtaacaacg cgaccgctca tcagtactaa tacatcattt
     7381 g
//
LOCUS       DAKISA010000163         7236 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.165, whole genome shotgun sequence.
ACCESSION   DAKISA010000163 DAKISA010000000
VERSION     DAKISA010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7236
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.165"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            6..476
                     /locus_tag="OVW45_004021"
     CDS             6..476
                     /locus_tag="OVW45_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCV5528348.1"
                     /translation="MSRVTAIISALVICIIVRLSWAVNHYRDNAITYKELRDKNAREL
                     KLANATITDMQQRQRDADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVPS
                     SAETSSASGMGNDATVELSPVAGRNVLGIRDGIISDQTALRMLQEYIRIQCLGG"
     gene            complement(565..1077)
                     /locus_tag="OVW45_004022"
     CDS             complement(565..1077)
                     /locus_tag="OVW45_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HCV5528349.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1272..2036
                     /locus_tag="OVW45_004023"
     CDS             1272..2036
                     /locus_tag="OVW45_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528350.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLGWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIESISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1987..3390
                     /locus_tag="OVW45_004024"
     CDS             1987..3390
                     /locus_tag="OVW45_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HCV5528351.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAAIIKLSWIEAAVDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLVADRFRNTFNAINNGEQYPVD
                     ELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAKREIPSPNVADAFIMAF
                     APIDTSLDIWEQLGRQA"
     gene            3390..4856
                     /locus_tag="OVW45_004025"
                     /pseudo
     CDS             3390..4856
                     /locus_tag="OVW45_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
     gene            4747..5490
                     /locus_tag="OVW45_004026"
                     /pseudo
     CDS             4747..5490
                     /locus_tag="OVW45_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
     gene            5494..6714
                     /locus_tag="OVW45_004027"
     CDS             5494..6714
                     /locus_tag="OVW45_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCV5528352.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            6718..7224
                     /locus_tag="OVW45_004028"
     CDS             6718..7224
                     /locus_tag="OVW45_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528353.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVN
                     LGSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDADG
                     NAEISWKI"
BASE COUNT         1923 a         1735 c         1973 g         1605 t
ORIGIN      
        1 cagcaatgag cagagtcacc gcgattatct ccgctctggt tatctgcatc atcgtccgcc
       61 tgtcatgggc tgttaatcat taccgtgata acgccattac ctacaaagag ctgcgcgaca
      121 aaaacgcaag agaactgaag ctggcgaacg caaccattac tgacatgcag cagcgccagc
      181 gtgatgctga tgcactcgat gctaaataca cgaaggagtt agctgatgcg aaagctgaaa
      241 atgatgctct tcggcgcaag cttgataatg gtggtcgggt gctcgtcaaa ggaaaatgcc
      301 ctgtgccatc ctcagccgaa acctccagcg cctccggcat gggcaatgat gccaccgtcg
      361 aactctctcc agttgctgga cgaaacgttc tcggtatccg ggacggaatc atcagcgacc
      421 agacagcatt gagaatgctt caggagtaca tcaggatcca atgccttggg gggtagcggt
      481 aattttactc attatccttc aaatcagatt ctgttgtcag aggaatgggg gaagctggat
      541 tcgcaagagc tttttatggg cagtttagct aaaaaaaggc aacacgaagc taaaactacc
      601 aacataaagt gctgaccagc tagcagctga aatcagactc actgtataca ctttcactgg
      661 atgaagttgc aacattcctg ccaccaaagg agcaatataa cgcagcacgg cgataaagcg
      721 cgaagtgaaa agtattgaca cagagttatt ttgcaactga aggcgaaccc gttctattgt
      781 attggaacgg ctcgatatta tacatgcaag ttgagggata tgcccaatta ttataccaag
      841 atggtaatta acgatagtac cgcaccatgc gccactcatg accgtgattc cagcttccca
      901 tggtgaaaga gtcgtcttgc tgactgtaat tacagtggcc atcataattg atgctggtgg
      961 caatatcgcc gaaatgagta aagtggattt tgtgaacgct attataaaaa gaagccccca
     1021 taggcgatat ggatgaagta caaaatagtt catcaaagca tttatccact ccatcaatgt
     1081 ctttccttct gtgaattttt ctttgctatt taaccgtgat acgacttaat ttaggcttaa
     1141 acaccttaca tttacaaggt actcctgtgg ggggccctgc cacggagcgt cgcaggagtg
     1201 agtgatgagg aagaattaaa gcaacctatt gtgacaggca gcgataacag taaatcacag
     1261 gaagcataat catggcaaaa ccggactggg aggccatcga atcggcatac cgggccggag
     1321 tccttagcct ccgtgatata ggcgagaaat acggcgttac agaaggggct atcaggaaga
     1381 gggccaaaaa gcttggttgg gcacgcagtg gcggtacgca ggtttgcaaa aatggtacgc
     1441 aaaaaaggaa agtgcgtacc agcagaaagc ctgccattac tggccttaca caaaaaagta
     1501 cgcaactaaa aacagaatct acaccggata cgaaaccgat acgcggaatg cgtaccgatc
     1561 ccccgactaa cccattccaa cccggtaacc agcaggcatt aaaacacggt ggttatgccc
     1621 gtcgccttct gctcaaagat gaggtgatag aggacgctaa agcgttgacg ctcgaggacg
     1681 aattatttcg ccttcgtgct aacaaccttg tcgctgcaga gaatattggt cggtggttgg
     1741 tgtcgctgga agatgctaat ggggaccagg aaaggaagat gctgattgaa agtatcagcg
     1801 ccgccgagaa agcaatgatg cgcaatacag ttcgtattga gtccatcgtt ggcacgcttg
     1861 cgacggtagg aaaaatattt gctgatacag cctaccgcaa ggccgccact gataaggtgt
     1921 ctctggaggc tgatcgtctt cgccgtgatg caggtattga tgatggcaat ggagagcgtg
     1981 acctcaatga cttctactct gacatccaaa ccgacgctga atccggcttt acgtagtttc
     2041 tggactatgc gggcacgtaa caaagtgctt tatggtggtc ggtcatcgtc aaaatcatgg
     2101 gatgccgctg gcattgccat atttctgtcg aataaataca ccctgcgttt ttgttgtgcc
     2161 cgtcagatcc agaataaaat cgaagagtcg gtgtataccc tgctcaaaat tcagatagac
     2221 aggtttggtc tgcggcaccg tttccgtatt ctgaacaaca aaatcattaa ccgggttact
     2281 ggctcggaat ttgtttttta tggattatgg cgcaacatcg aagaaattaa gtcactggag
     2341 gggatcgatg tgttgtggct ggaagaagcc cacgcactga cggaatacca gtggaaaatt
     2401 ctggagccaa cgatccgtaa agagggttcg gaatgctggt tcatattcaa ccccggactt
     2461 gttactgatt tcgtctggcg caacttcgtt gttgatccgc ccgaaggcac tctcatccgc
     2521 aaaattaact atgacgaaaa tccgtttctg tctgacacca tgcttaaggt tatcgacgcg
     2581 gcgcgacgcc gtgatccgga tggttttaaa catgtgtatg agggcgttcc ggagtctgat
     2641 gatgatgcgg caatcatcaa actgtcttgg atagaagccg cagtggatgc gcacaaaacg
     2701 ttaaatttcg aacccagtgg aagaaagcgt attggctttg acgtggctga cagtggtaca
     2761 gataagtgcg ctaacgttta ccgtcacgga tccgttgttt tctgggccga cgaatggaag
     2821 gccaaagaag atgaattact gaagagctgc cagcgtactt atcaggcggc gctggagcgt
     2881 gaagcagata ttgtttacga ctctatcggt gttggtgcgt ctgccggtgc taaattctct
     2941 gaaattaacg ctgaccggaa gagcgagaac gcatacgcgc gacgtgtgaa ttaccagagg
     3001 tttaacgccg gtgctggtgt gcatgagcca gatgacgagt acaacggcat ccccaacaaa
     3061 gactttttcg caaatcttaa ggctcaggca tggtggctgg tggctgaccg tttcagaaat
     3121 acgtttaacg ccattaacaa cggagaacag tatcctgtgg atgagctgat cagcatagat
     3181 tctcgttgtc cgttgcttga aaagctgaaa ctggaactga caacacctca tcgtgatttc
     3241 gaccgtaacg gacgtgtgat ggtcgaaagt aaaaaagacc tcgcaaaacg cgagataccg
     3301 tcaccaaacg ttgctgatgc attcattatg gccttcgcgc caatcgatac atcgctggat
     3361 atctgggaac agctggggag acaggcctga tggcacgaaa caaacaagcc ctgcggcgaa
     3421 ctgtgcaggc cacagctgat ggttatgaga attttattgc ccgcgtaggg atgcagacac
     3481 ctaaccagca ctcagcatcc acctaccggg ctaatttcac cagtcgtaac cgcatgctgg
     3541 tggaatggtc ctatcgttca tcctagatca tcggcgaagc agtcgatgct atcccggatg
     3601 atatgacccg caaaggcatt cgcatcactt cggaaattga tgcaaaagat cgtggcattc
     3661 tcgaatcaca actggatgag ttgcaaatct gggatgcgct gaatgacgtg ctgaaatggt
     3721 cgcgcctcta cggcggcgcg gtgggtttca tcatgattga ggggtaggca ccaatgaccc
     3781 cgctgcgacc cgaaaccatc ggtaagggca agtttaaggg gattctcccg ctcgaccgct
     3841 ggatggtcga cccggcactg acccgccgca ttaaagatat ggggccggac ctgggtaaac
     3901 ctgagtttta cgatgtggtg accacagcaa cgggaattcc tgcctggcgc attcatcaca
     3961 gtcgcctgat tcgctttgat ggcgtcacgt tgccatttca gcagaagatg accgagaacg
     4021 aatggggaat gtcggttgta gagcgtatct gggatcgtct taccgcgttc gacagcgcta
     4081 ctgtcggcgc ggcgcagctg gtctacaagg cgcatttgcg tacctacagc gtggagaagc
     4141 tacgcgagct tatcgcactt ggtggtcctg cgtatgaagc gttgctgaag aatatcgacc
     4201 tgattcgaca gttccagagt aatgaaggca tgacgctcat ggactcgcgg gataagtttg
     4261 aaacccatca gtacagcttc agtggtctgg atgacatcct ttcgcagttt gtagaacaga
     4321 ttagtggcgc tgttggtatc ccactggtgc ggttgttcgg acagtccccg aaaggatttt
     4381 ctaccggtga tgcagacctt gccaactatt acgatcgcat cagttcgttg caggagaggc
     4441 gtttacgtct tccggtgcgg cggatactgg acatcatgca tcgttcggaa cttggcaagc
     4501 cgctgccgga cgatttcacg tttgagttta acccgctctg gcaaatgtct gatgtcgatc
     4561 gctcaacggt ggcgttaaac accaccaacg caatcagtac ggcgctgggt gatggtctga
     4621 tgacactgaa agccgctatg actgatttgc gagaaaattc tgacgtaacc ggcatcgggg
     4681 catccattac cgacgaggac atcgagaatg ccgaagatga agcgccgccc ggcatcggcg
     4741 aacctgatga cgaaccgcag gagccgtcag gcggaaatcc ggtatcgaac cagcctacgc
     4801 aggatagcga gggcggtcgg agacatcgta aatggtcgct acgatggttc aaatgacagt
     4861 atcacggaaa ttattgcggc gctggagcgt tacagtgaaa tcatcacccc ctaggcgaca
     4921 aaggtcgcgg aaaactttac tgcggaccta acccggcaga acgagaaagt ttggcggcaa
     4981 cacagcaaga acatcagtcg cgagctccgc aatcttgtgg aaagcgctcc tgtgggccag
     5041 gtgatgcaat ccatcatcgc cgaacaggtc aagtacatca aatctctgcc tctcgaggcc
     5101 gcagacaggg tgtacgacat ccagaacaaa gcgatagagg ctgttgtcac cggtggtcgg
     5161 gcggagcagt ttgctaaaga gattgcatct accggcgatg ttgctaagtc tagggccgat
     5221 ctgattgccc gaacggaact gggaagagca acgggcgcgc tggatatggc ccgagcgata
     5281 gctattggct cggatggtta tatttggcgt acagccgatg atggcgatgt ccgagattcc
     5341 cacgatcaca tgaaaggtaa attcgtccgc tgggattcac ctccaacttt ggacggcatg
     5401 accggccacg cgggcgagct gccaaactgc cgctgctata aagagatcgt gtttgttcgc
     5461 gttccattcg caatgaaaag ggcagcataa cccatgaaat acttttttga gaccaggctc
     5521 ggggaaaccc gataccgcct ggctgacggc tcgttgctgt gcaaagacgt gccgatagga
     5581 cgaacaggta agcagctcta tggtgctgat gacctgccaa aactgaaacc cgataagttc
     5641 ggtgaaatag tcgtcacgcg ttctcctgag caggtattcc atccggccac gcttgcctca
     5701 ttcgaaggga tgagcatcac gatcctgcat cctgaagatg aaaacgggaa tgtgcggctg
     5761 gtaaatcccg agaactggaa agagcttgct gtcgggcacc tccagaatgt ccggcgcggg
     5821 acgggtgagc agtctgattt gatgctggct gaccttatcg tcaaagacga aagcgccatt
     5881 cagcttatcg aagatggcct gcgcgaagtg tcgtgcggct atgacgcgga gtacgagcag
     5941 accgagccag gtaaagccga gcaggtcgat attaccggaa accatgtggc tcttgtccct
     6001 aaaggcagag ccggaaatcg ttgtgcaatt ggagacagag acacaatggc aaatcaaaag
     6061 aaaaactggt ggaaccgcat gcgtgcggcc atcaagacag gagatgccga caccatgaac
     6121 gaactggtgg agtcggctcc cgcatcggtt acaggagatg agggggattt gccgcagggc
     6181 gttaatctca acatcaacct gtccccgcag caaccactac cggacaaagc accagagatg
     6241 ggcggagatc caaccggcga cagtgatgat gacctcaaaa cattactgaa agccctgctg
     6301 gctaagctgg aaagaaatgc cacgggcgat aacgataata agcctgacga taatccgacc
     6361 ggtgacggcg aggacgatga agaggaaacc acgattactg gtgactcagc ctggcgtgcc
     6421 gaagttatcg ttccgggtat cgatctgagc cgtaagatga aaccgaccgc gttcaaacgc
     6481 gaggttctgg cttctgctga caaaacgctg gttcgccaga tagtcggtga tgcggatatc
     6541 cgcaaattac cgaaacaatc ggtcgacatg gcgtttaatg ccgtgtctga ggttgccaaa
     6601 gggcgaaaca cccgcgccac caccggcgat gcacagcgcc taaacatggg catgaccagt
     6661 atcgcttccc tgaacaaaca aaacgctgaa ttctgggcaa accgtaaagg gtaaaaaatg
     6721 aataatgtat ttctgtaccg gatgcctgtt ggcattgccg gggctgtctc tcgcccgcag
     6781 gacttaaccg tcgaaccggt ggtccttaaa tccgataacg ccttcgctgc ctatggcctg
     6841 gctggtaaat acgatgatga cggttttttc gtgccgctgg cagatggtga taccgcagac
     6901 aaggtgaagg ggatctacgt gcgcccttat ccgaccacgt cgcagccgga catggttcgc
     6961 caggtgggaa caggcaagaa cttcccgggc gacgccatga agcgtggcta cgtgaccgtt
     7021 aatctcggtt ctgattttga tgccagcact atcaaaaaag gcgatccggt atacgttgtc
     7081 gtctccactg atgaatccat caaagtgccg ctgggtggat tcatgtccac gtcagtcagt
     7141 ggcaaaaacg tggtgctgac caacgctgaa ttcacaggtg ccggtgatgc tgacggcaat
     7201 gcagaaattt cctggaagat ttaaggaaca gacgaa
//
LOCUS       DAKISA010000164         7213 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.166, whole genome shotgun sequence.
ACCESSION   DAKISA010000164 DAKISA010000000
VERSION     DAKISA010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7213
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.166"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..4275)
                     /gene="traI"
                     /locus_tag="OVW45_004029"
     CDS             complement(<1..4275)
                     /gene="traI"
                     /locus_tag="OVW45_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HCV5528354.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGSDLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IEAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHA
                     VVTNVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSRTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETGFDIRAYRDAAEQRAYTRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     MYTDLLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVTERVTAQSHSLT
                     LRNAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVSDSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFADLGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMQKGEAFPDV
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGTGDRIRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKL"
     gene            4357..4584
                     /gene="vapB"
                     /locus_tag="OVW45_004030"
     CDS             4357..4584
                     /gene="vapB"
                     /locus_tag="OVW45_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCV5528355.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSADFMDNREQPGMQERESF"
     gene            4584..4982
                     /gene="vapC"
                     /locus_tag="OVW45_004031"
     CDS             4584..4982
                     /gene="vapC"
                     /locus_tag="OVW45_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCV5528356.1"
                     /translation="MLKFMLDTNICIFTIKNKPASIRERFNLNQGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(4991..7162)
                     /gene="traD"
                     /locus_tag="OVW45_004032"
     CDS             complement(4991..7162)
                     /gene="traD"
                     /locus_tag="OVW45_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HCV5528357.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLV
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIRSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTYLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQ
                     PQQPQQPVSPAINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMA
                     AYEAWQQENHPDIQQQMQRCEEVNINVHRERGEDVEPGDDF"
BASE COUNT         1503 a         2215 c         1842 g         1653 t
ORIGIN      
        1 cagtttccgg ttaccttccg tgccttcaag cgcgatggca aaggtttcac tcgccccctg
       61 tgcaccgtgg gcggtgatgg cataggccag gtcaatatgt tgctctgccc gctcctgacc
      121 ggggcgaatc acacgggtct gctgaccgtc agaaagtgtg acactgtcac cggaaaccgc
      181 tgtcaccgtc cagacgctgt tggccacata accgcgctcc cggtcactct tcgtgaagcg
      241 tatcctgtca ccggtcccca cccggatggt gtccggggtg tacagcgtga cgccttcagc
      301 caccgcctcc cggggcgaaa tcagccgcgt gttaccttcc gcatcctgca gggttatcag
      361 cccgtcatcc ttgctgatac cggcaatccg gtgatacaca ttatccacca gggcaagtgc
      421 gtcccgatgt gtctcccagg tggagagacg acgcagctcc ccgtcgcgta tattcgctgt
      481 gttcaggaca ggcaccatga cctgctcttt tcccagctca ccggcctttt cccgtgcatc
      541 atgaatcatg ctgttcagta cgcgccggtc ctcattcagg tgcgtgacaa tcagcgtctg
      601 ctcccgtgct tccggtgtcc tgccggtata gtcgcggaca atggcctcat acagtgtcat
      661 ggggacatcc ggaaaagcct cgcctttctg catcgccttc tgctgcgcct ctgccagttt
      721 cgcttcctgg ctgtgactga actccgtcac ggagtgctcc ggtgcccatg cgccctccag
      781 acgtggcacc tgagacggtt tcacactctc aagcccggac agtgcccttt ccacatcccg
      841 gttaatcagg ctgtataccg cctcccgcag ttccggcgtc tgacgcacaa tctccttcat
      901 gatggccaca tcggcagcac tgcgcgtctg ctgcagacgg aagggctggc cgggcgcgat
      961 ggcctgcagc tggtccgtgt caccgctggc cacggcacga ccaccaccgg ccgcaatcag
     1021 ggcgtatgcc cgtgccatgt cggtgttacc caccattgag ctctcatcga gcaggaacag
     1081 cgtgttgctg aaatccggcg tttctccgct gcgctgctgc agctgcgtgt catgcagaaa
     1141 ggacgccagt gtctgtgcat ccacgccggc gctgcgcatc tccccgaccg cacggtgtgt
     1201 gggccccagc cccacgacac ggggacgttc actctccggc agcatgttca cggctgacat
     1261 caccgcccgg aactgtgtgg tcttacccac accggcatag ccctgcacca ccgtgaaacg
     1321 gtcggacgtt tccagtatca tacgggtggc ggcgcgctgt cctgatgtca gtttctccat
     1381 gagttcgcca ggtactctct ccatcagcgg cgtgaccgcc tccttacctt cgagaatatg
     1441 gcgaagaatg ctcttttctg cctcatacga cgcacgggaa accagcaggc ctgtgccata
     1501 gccttttgcc acatccacat acagtaaatc accccgtttt atctgcgcat tgatttcccc
     1561 tcccaggtca gcaaaaccgg ttccttctgc agcaaacgac ttcgcctctg tcagcaggtc
     1621 caccatgctg aaggccaggt ttttactttc cagcacagga agggccagat gaatggcctg
     1681 ctgtgccggc gtgtgaagcc cggctttctg cagactgata gcggtttcca gcaatgtctc
     1741 accggcacgc gcctttatct gctcagaaac caccgtaaag gagggatggc gggcaagttt
     1801 ttccgcagta cgggtttcat ccagtgagga atacagccgg acatcacgac cactgcgggc
     1861 cagaccgttc agggtggcat tgtccattgc catctgtgtg acggaggcaa aaaccgtcgc
     1921 actgtcgctg acggaatgcc cgggcgtttc cacccagccg ttctccagct tcagtgccgt
     1981 gaacggtgaa tcgctcacag gcagggtggc cggttcagcc cgtcccggca caacaaccgt
     2041 catcgcatct tcactgacgg atgccacctg caggcggtca ccgccggaga cgcggagtcc
     2101 gggaattttc cctgtcaccc tcagacgctc accgtctgcc accggcattt tttccggccg
     2161 gaacagcgac cagctgctgt ccagggaaga aatacgcacc acctgcgttt cgccctgcgc
     2221 attccgcagg gtcaggctgt gactctgcgc cgtcacacgt tctgtcacat agcggtcatg
     2281 actgcgtgtc tccgggttcc actgctccat caccattccc gggcggtaca tatcccgcag
     2341 ataacggctc cggctgtcca gccagaccgg tgacagggcg gtcatggtta cctccgggtg
     2401 tccgagcacg ccctgtgttt tcagctcact gcgaatggcc tgtgtcagta tggcctgttc
     2461 ccgtactccg ctgacctgtg ccacactctc ttctccggct ttcacgctgg ccgcaaaatc
     2521 tccggccagc cgggcatagc ggacattacg gtccggttca ctgatgatgg tggccggtcg
     2581 ctgttctccc ccctgccagc gatatgtgtt caccccggca tccttcatgg ccatcagtgc
     2641 actgcctgta ccggttcgct gcccgctgtc ggttatcagg acctgtacgt tatgacgtgc
     2701 ggcaccatcc agcagggtta acgtctcttt cagggagagt ttttcgccct ggtcgacgat
     2761 aacggtactg cccggcgtga aggccatgcc ttccagcaac tgacgacgtc cggttatcag
     2821 ttcaccggac agccgttcat cctgcttcag gttcatctgc gagcgacggt cagcagcgat
     2881 aatctgcacc tcccgcccct gctcccgggc catcatgacc agttcagcca cccgctcacg
     2941 ctgcccggct gcaccgccct gcccggacac aatggccagc gacggacggt cctgtgccag
     3001 aacactgacg gcatcgctgt aaccggccgt ccgggggaca cttttctccg gatgtacggt
     3061 cacccggttc tgtttcatga tgtcacgact gagtgcccgg actgacagct catcgagcac
     3121 atgaatcccg gacgtgaaca gccccttttc acggtcaagg gggatgagct gctcacggct
     3181 gatggcctca tcgataccgg cgcgtgcccg ttcaatcaca ccattttccg gcggcagtat
     3241 gccgaccgtc ctggccagca ggtccgtata cataaactgc actttgcgtt cacttaatcc
     3301 ggcaatggcc tgtgtcaccg cctgctgcac atccggcccg tcctgtgaag cgggtccggg
     3361 tgtctgcgtg cgggtatatg cccgttgttc cgctgcgtca cgatatgccc ggatgtcgaa
     3421 cccggtttcc ttcagcgtct gcatccactc ggccattctg acttcaggat cgacgtgctg
     3481 tttggattta cgcgtatcca gcgccgccac atcccgggat ttcagcgagg cgtcttcccc
     3541 gacggcctcc cggatagtcc gtgagcgtcc ggaaaaggcc tccaccggta cacccggcat
     3601 ttcccacata ccgtgcttac cgaccacttc agtttcatag cccagcgcct caacctgctc
     3661 tttcagtttt tcccggtaga gcctgccaaa ggcaatctga ttagcgtaca cattctcaat
     3721 gaaccccgtt ttccccactt tgtcactgct cagtgtcttc cattcgccgt tatgctgcgt
     3781 aacattagtc accaccgcat gcgtgtgtaa ctgtggttcc tgatcgcgac tggtgtcgtg
     3841 gttaaacagt gccatcacca gattaccggt cagcaccgtt tctgactgtc cgtccgtcat
     3901 cacccgtgtg gaggccagtg cctccacctg acggacagca aaatccacgg cctggttatg
     3961 tgcttcaata agacgtttgt cccccccgag catggccatc atggagacac ttttgggagc
     4021 ggagaaggtc aggtcgtagc cgggacgatg cttattactg ccatcctgca tgcggcttaa
     4081 atccgaaccg tccggcagcc tgccctccag aagacgggta aagacatcct tatcgacact
     4141 gccctgcagc cccagttgtt cagccccctg gccggcccag cgttctccca tgctgcccag
     4201 cacatagtaa ttatccttgt cggtataata attaccggca cttccggccg atctgacctg
     4261 cgcgatactc atcataaacg tatatccctt tgacatatcc cggtatcaat cccacaatgg
     4321 atatacacaa gacatatcca cataaggagg caaataatgg aaaccaccgt atttctcagc
     4381 aaccgcagcc aggcggtcag actgccaaaa gcggttgcat tgccggaaaa cgtaaaacgc
     4441 gttgaagtga ttgctgtcgg acgaaccaga atcattacgc cagccggaga gacatgggac
     4501 gaatggttcg acggacacag cgtcagcgcc gattttatgg ataacaggga acagcccggc
     4561 atgcaggaaa gggagtcatt ctgatgctga agtttatgct cgataccaac atctgcattt
     4621 ttacgataaa gaacaaaccc gccagcatca gggagcgttt taacctgaac caggggagaa
     4681 tgtgtatcag ttcggtcacc ctgatggagc tgatatatgg tgcagaaaaa agccagatgc
     4741 ctgaacgtaa tctcgctgtg atcgagggat ttgtttcccg cattgatgtt ctggattacg
     4801 acgctgctgc agccacacac accggccaga taagagcaga acttgcccgt cagggacgcc
     4861 ctgtcgggcc atttgatcaa atgatcgcag gtcatgcccg cagtcggggg ctgattattg
     4921 tgactaataa cacccgggaa tttgaacgtg tgggcggcct gagaactgaa gactggagct
     4981 gacctgttca tcagaaatca tctcccggct caacatcctc cccgcgctcc cggtgcacat
     5041 taatgttcac ctcttcacaa cgctgcatct gctgctggat gtccggatga ttttcctgtt
     5101 gccatgcctc ataagcggcc atatccacca cttcaccgga ttcactgatc ccgggtggca
     5161 gttgctgttc catctcttct tccggtttca ttttcagctc ctgctcgatc ccccctgccg
     5221 gaacattcac acctgaatct gacttcttat cgttgatggc aggagacacc ggctgttgcg
     5281 gctgttgcgg ctgttgcggc tgttcagcct gagtcacgtc ttctccggaa acaacctccg
     5341 ggacatccgg ttcgaagagg ctggccatct gacgaccttc tgcttccctt gcggcaagta
     5401 cggcactcag acggttctcc atttccgggt tgatgtcacg cggaataaac tccggagcga
     5461 ccttcggtcg tgcctgatat ttcagtgaga gttttactgc cggatacggt ccgggcaggg
     5521 tgacataaca ggtcagatcc ggcagggact gaatgtcgga ataactgacc agcgtctggc
     5581 gctccatatc cttaccggtc gataccccgt cacgtaccgg atcagcaccg taggaatact
     5641 gctcgctggc tttcaggtgc tctttctcac caatctcacc ggcagcgaac tctgcaatct
     5701 tatggctggg agaacggaaa aaggcacggg tgttcatgac atcaaacaac gtggcagccg
     5761 ctttctcacc gtagatatct tccagctggg cataggactg gataccaaac acataacagc
     5821 caccgaactt acgggcttcc ggcaggatct ccaccaggtc cggcagtttg tgtaacgtgg
     5881 gtaactcgtc acagaaaaac cacacacgac ggttacggtt ttctcccatt gccagcagac
     5941 cacgaatggc aatggacagc cacatggaga tcacaggctt cagggaggca tgggtgtcgg
     6001 cgttcgacga aataaacagc cagccgtttt tctgatcttc ccggacaccc cgcatccagt
     6061 cacgtatggt gaagggttca ccgttatgct caatcccctg caggtaacgg atggctttca
     6121 cgtagttggt cagcacagca cggatggaaa tcgccgtttt ctcaattttc tcttccacca
     6181 gattggccgc cggtgaatta cgcaggtagg tacgcagttt ttcaattttg atggaaagca
     6241 gtgtgtccac cagtttgctg tagctgcggt tggggtcatt acgcatcaga tacgccgctt
     6301 ccgcaaaaat ggtacgtcct gaaccctgcc agaacgggtc ctcttttgtg cccatcggga
     6361 tcagcgtgtt cgcggtatta tcaaaatccg gctgtgtcag gcactccttc cacaaatccc
     6421 aggcggcaca gcgggcatcc agcggattca ggattttatc gatggaggga tcatagtaac
     6481 ttttaacaaa ttcccctgaa cggtcataaa tcaccaccat atctccacgc tgacgggcgt
     6541 agttggccag acgacggata acttccgact taccggcccc gaccgtgccg tgcagacaga
     6601 agttctggat ttcagaatcc cggataatcg gcaggtcgcc aatccgtata tcggagtcct
     6661 tgccgtcttt tttcagcatc cgggcaacgt ctttcggatt gtctgtcagc tgacgaccac
     6721 ctgtgacttc attttcgctc tgttgtttac cctgacgccc cagaatccag gagacaacaa
     6781 agaaggtaat caggcaaata accagtgcca caactgtggc cagaacgaat gcggaccata
     6841 gctgctctcc gcaccagatc atatatttat catgcagtac ctgagcagca ttcatccgga
     6901 aggttttgcc gtaatactgg atctcatata ccggctggga cctgattaaa tcccgcatgc
     6961 cttccagcgt ggtacaccac cagtaaatac agccgttcac aaacgtctgc cagcttattt
     7021 ttacccataa aaccagacca acgagtatcc agaaaaaaat aaacaggcag taaagcatga
     7081 tattggcgat ctggctgaac atgcggatac gcatggacgc aatctgaccg ccctgggtca
     7141 tatcctttgc gttaaaactc atgatgatat tccgaagaaa aaaagtcagt cggactgatt
     7201 attaatgatg aaa
//
LOCUS       DAKISA010000165         6936 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.167, whole genome shotgun sequence.
ACCESSION   DAKISA010000165 DAKISA010000000
VERSION     DAKISA010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6936
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.167"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            40..252
                     /locus_tag="OVW45_004033"
     CDS             40..252
                     /locus_tag="OVW45_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528358.1"
                     /translation="MSNTQKIINTEKYNEWVKKFSEQIFKITGDENVAKNELEPWTPE
                     GNAPNYCWWEVDPVDAANEAMSYHND"
     gene            285..503
                     /locus_tag="OVW45_004034"
     CDS             285..503
                     /locus_tag="OVW45_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCV5528359.1"
                     /translation="MTTLFRKEYPQKSRATEFLFLILFIVLMIPISPLIFVWAIGKII
                     EPVTELYTDVVWASFNTLHNKINPYKEN"
     gene            658..891
                     /locus_tag="OVW45_004035"
     CDS             658..891
                     /locus_tag="OVW45_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001726830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCV5528360.1"
                     /translation="MVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQRSLSERLFRE
                     QGVDTKILLGHSNQKMTDIYNDVRGKEWKKLVI"
     gene            complement(924..1562)
                     /gene="ompW"
                     /locus_tag="OVW45_004036"
     CDS             complement(924..1562)
                     /gene="ompW"
                     /locus_tag="OVW45_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCV5528361.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDAGSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            1802..1891
                     /locus_tag="OVW45_004037"
                     /pseudo
     CDS             1802..1891
                     /locus_tag="OVW45_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            1919..2662
                     /locus_tag="OVW45_004038"
     CDS             1919..2662
                     /locus_tag="OVW45_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCV5528362.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            2692..3231
                     /locus_tag="OVW45_004039"
     CDS             2692..3231
                     /locus_tag="OVW45_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCV5528363.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3336..3734
                     /gene="yciA"
                     /locus_tag="OVW45_004040"
     CDS             3336..3734
                     /gene="yciA"
                     /locus_tag="OVW45_004040"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCV5528364.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(3774..4493)
                     /gene="tonB"
                     /locus_tag="OVW45_004041"
     CDS             complement(3774..4493)
                     /gene="tonB"
                     /locus_tag="OVW45_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCV5528365.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            4584..6104
                     /locus_tag="OVW45_004042"
     CDS             4584..6104
                     /locus_tag="OVW45_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCV5528366.1"
                     /translation="MRTVITYLRFSSAIQGAEGADSTRRQNDLFKQWLKKNGDAQIVA
                     SFSDEGLSGYKGKHLTGQFGDMLARIEAGEFPEGTILLVESIDRIGRLEHLETEALMN
                     RILGNGIEIHTLQDGLIYTKDALADDLGISIIQRVKAYIAHQKSKQKSFRVSQKWGQR
                     AKLALAGEQRLTKMVPGWIDPETFKLNEHAETVRLIFKLLLDGESLHNIARHLQSNGI
                     KSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEGVVDIPTFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKNKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDNMRRKASLTDKELPQLKDIDLMTKAGRVECQLI
                     LSKHLKGLTLGKDSVTVTLQNDTEITIPTNPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            complement(6137..6907)
                     /locus_tag="OVW45_004043"
     CDS             complement(6137..6907)
                     /locus_tag="OVW45_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528367.1"
                     /translation="MFDTKQIRYSGKDGIYFHLRDNVESFLALTATEQMDYDSPMMVT
                     TQAMQSGQTITDNVQRAPKTISISGVVVVDSTGSVLLTRQGQLVETFISTVEQWRDQK
                     QIITIVCRDGIAITDSIITQFKASKEHTIANGLRIQMTFQGVEFRSIVGQTDVSAATG
                     KTATTNDGGATSKKNTGNTTTSLGSPMLTCKELFGYSASELSDEALKARVTCSKSVSV
                     KNGESTFSDAANEQASKVLKPGNALRKYSVNPNKKGTY"
BASE COUNT         1831 a         1578 c         1629 g         1898 t
ORIGIN      
        1 actgtaaata aaatttaatc cttaactgga ggtatatcta tgtcaaatac acagaaaatt
       61 attaacactg aaaaatataa cgagtgggtg aaaaaattct ctgagcagat ttttaaaatt
      121 actggcgacg agaatgtggc aaaaaatgaa ttagaaccgt ggacacctga aggaaacgca
      181 ccaaattatt gctggtggga ggttgatccg gttgatgctg caaatgaagc catgagttac
      241 cacaacgatt aatgtcggga ggccgcccga aagggcggta agaaatgact acattattca
      301 gaaaagaata tccgcaaaaa agtagggcga cagaattttt gtttctcatt ctgtttatcg
      361 tattgatgat accgatatcc cctctaattt ttgtctgggc aatcgggaaa ataattgagc
      421 cagttactga attgtatacc gacgttgtat gggcgtcgtt caacacactg cacaataaaa
      481 ttaatccgta taaggaaaac tgatatggca actttgacaa aaaaagaacg ggcatggttg
      541 aacgaattac aggaagttct tgatcgctgt cctgtggtgt catcctgccg cgattgcgtt
      601 cttagtccgt ggctattgca ccaccatcac gcgaaaggga cagctaagcg cggcgggatg
      661 gttaagccag caacattaac cgttgcattt aaaaaagccc gggattctgt ggattacaac
      721 tggcgtgcta atggcacccc accctctttc catgagcaga gatctttatc agagcgattg
      781 ttcagagagc agggggttga taccaaaatt ttgctaggcc attcgaatca aaaaatgacc
      841 gatatttaca acgatgtacg cggtaaggag tggaaaaaac tggtcatttg ataaccagtt
      901 ttgcagaggt tttgcagaga aaattaaaag cggtacccgg cagagaacat aaacacccac
      961 ggatccagtc gtaccgagtc ttttacggta gtaacaccag atttgtactt agccgtggta
     1021 tcgatatcca tgtaccacac tgacatgtta accaaccagt cacggttaat cagataatca
     1081 acccccacct gcccggcagc tccccaggaa tctttcagac tgagatcgga aagccccgcc
     1141 tctttgccat gatcgttaaa tccattatca aagaaggtgg tgtagttaat acctgcccca
     1201 acataaggac ggaatttgct gccggcatca ccaaaatacc actgcgccat cagtgttggc
     1261 ggcagatgat gaacggttgc aatatcgccg gtcgcccggg tgccgatttt atggcggaac
     1321 ggcgtcgctg ccagtaattc cacaccaatg ttgtcggtcg ccatataagt aaacgtcagg
     1381 cccagttgcg tgttattggt cacgctgaat ccacccagac ttcctaacgt accaccagca
     1441 ccttctgttg gacgtacggt tgcagaacct gcacgcataa aaaattcgcc tgcttcatgc
     1501 gcaaaggcac tgccagagag aagagttgtt actgccaaag ccgccactgt taactttttc
     1561 atatccgctc cgtcgttatg gttataaaaa cgtgacgaat atacctacat tggagtaaca
     1621 aatgatccta catagatcac attacatatg ataattcacc ctttattgat ctggattaat
     1681 ttagaagttg cattgagaat cctggttaac gtgatttaaa tcaattttta gatattttcg
     1741 ctacgcaaat attaactcgt tcgccacttg ccgcggtttg cccacgcctg aaggccagca
     1801 attacctccg ctttacaaag atatgctttt ccatacaaga cctgatgctg ccacccgagg
     1861 cttaaccagg gtacaattgc ccgctaatta acacctgcat aaaactcaag gagagtgcat
     1921 gtctatcacg gcgcagtccg tataccgtga caccggaaat ttcttccgta atcaatttat
     1981 gaccattctg ttggtatcgt tgctatgtgc gtttatcaca gtggtgttag ggcatgtttt
     2041 ctcacccagt gatgcacagc ttgcgcagct caatgacggc gtgcccgtta gcggcagtag
     2101 tgggttgttc gacctggttc agaatatgtc accggaacaa caacaaattt tgctgcaggc
     2161 ttcagcggcg tccacttttt caggattaat cggtaacgcc attctcgccg gaggcgtaat
     2221 attaattatc cagctggtgt ctgcgggtca gagagtcagt gcgctgcgag ctattggtgc
     2281 cagtgcgccg atattgccca agttatttat tctgattttt ctgactaccc ttttagtaca
     2341 gattggcatc atgctggtgg tcgttccggg aattatcatg gccattttac tggccctggc
     2401 accggtgatg ctggtacagg acaaaatggg catttttgcc tcgatgcgta gcagtatgcg
     2461 gctgacttgg gcgaatatgc gtctggtggc acccgcagta ctgagctggt tgctggcaaa
     2521 aacactgttg ctgctttttg cctcttcttt tgccgcatta accccggaaa ttggtgccgt
     2581 actggcgaac accttgagca acctgatttc agccatattg ctcatctatc tgttccgcct
     2641 gtatatgttg attcgccaat aaccttaagc atttgattac ggaatcgtaa aatgaagcag
     2701 tttcttgatt ttttaccgct ggttgtcttt ttcgcgtttt acaagattta tgacatctat
     2761 gcggctactg cggcgctgat cgtcgccacg gcgattgtgc ttatctatag ctgggttcgc
     2821 tttcgtaagg ttgagaagat ggccctgatc acttttgttc tggtggtcgt cttcggtggc
     2881 ttgacgctgt tcttccacaa tgatgagttt attaaatgga aggttacagt catttatgct
     2941 ctgtttgcgg gagccctgtt agtcagccaa tgggtgatga aaaagccgct aattcagcgg
     3001 atgctgggta aagaactcac gctgccgcaa ccggtatggt cgaagctaaa tctggcctgg
     3061 gctgttttct ttatcctttg cggtctggca aacatctaca tcgcattctg gctgccgcaa
     3121 aatatttggg tcaactttaa agtctttggc ctgaccgccc ttaccttaat cttcacattg
     3181 ttaagcggta tctatatcta ccgccacatg ccgcaggaag ataaatccta accagactgc
     3241 cagtccttcg gggctggcgc tgtatctcct ctgttctcct caggaaaatc atagtagcat
     3301 cgcgcctgtg attttccttt taagtcggtt ttaccatgtc tacaacacat aacgtccctc
     3361 agggcgatct tgttttacgt actttagcca tgcccgccga taccaatgcc aatggtgaca
     3421 tctttggtgg ttggttaatg tcacaaatgg atattggcgg cgctattctg gcaaaagaaa
     3481 ttgcccacgg tcgcgtagtg actgtgcggg ttgaaggaat gactttctta cggccggttg
     3541 cggtcggcga tgtggtgtgc tgctatgcac gctgtgtcca gaaagggacg acatcggtca
     3601 gcattaatat tgaagtgtgg gtgaaaaaag tagcgtctga accaatcggg caacgctata
     3661 aagcgacaga agcattattt aagtatgtcg cggttgatcc tgaaggaaaa cctcgcgcct
     3721 tacctgttga gtaatagtca aaagcctccg gtcggaggct tttgactttc tacttactga
     3781 atttcggtgg tgccgttaat tttaaacagg atattcacca caatcccact gcctggctta
     3841 cccggctcat aacgccatct gcgcatcgca tttttcacct cacgctcaaa catgttcgca
     3901 ggcttggctg agaggatttg tacgttatcc acgcgaccat ccggcgtgac gtcaaattta
     3961 actttaacct gcccttcaat gcgcaatgcc tgtgctcgtg ccggatactg cggctgatta
     4021 cggcttaatg cgcgtggtcc tgaagccaca ctggtaaccg gcttgctggt tgcagccgtt
     4081 gccgtacttg atgtggggcg cgcgggtgcc gtattttcaa acggtgatgc cggacgcgac
     4141 tctacgggtt tgacatcgcg ttttggctgc tcctgtacct ttttcaccgg cttcggtttt
     4201 ggcttaggtt tcggcttcgg cttttcaatg accaccggtg cttcttttgg cggttcgggg
     4261 atcggctcag gttccggttc tggctctacc accggctccg gtggcggctg aacggcttgt
     4321 ggcggttcga gatcagcagg cgcaaccatc gtgacagaaa ttggctgcgc aggcgcaggt
     4381 agttcaataa cctgatgtac cgaggtatag agcagacccg ccacaacagc accatgaatg
     4441 cagaccgaaa gtaacgtcgg ccaggggaag cggcgaggta aatcaagggt cattgtattg
     4501 tagctattcg gcattgtagc attagccata acgtttataa gttcatcttt aaatgataat
     4561 caattgcatg ggagtttatc accgtgagaa ccgtaataac atacttgaga ttctcatcag
     4621 ccatacaagg cgctgaaggt gcagattcaa ccagacgaca aaacgacctg ttcaagcagt
     4681 ggctgaagaa gaatggcgat gctcaaatag tagcgtcttt cagtgatgaa gggttgagtg
     4741 gttacaaagg taaacacctt actggtcagt ttggtgacat gttagcccgt attgaggctg
     4801 gggagtttcc agaaggtaca attctgctag ttgagagtat cgaccgtata ggccgattag
     4861 agcatcttga aacagaagcc ttgatgaacc gcatcttagg caacgggata gagattcaca
     4921 cattgcaaga tggccttatc tacacaaagg atgctttagc cgatgacctt ggcataagca
     4981 tcatccagcg tgtcaaagcc tacatagcgc atcagaaatc taagcagaag tctttccgtg
     5041 tttctcagaa atgggggcaa cgtgcaaaac ttgcccttgc tggtgaacaa cgtcttacaa
     5101 agatggttcc tggatggatt gaccctgaga cttttaaact caatgaacac gctgagactg
     5161 tgagattgat attcaaactg ctgctagatg gtgaaagtct gcataacatt gcacgtcacc
     5221 ttcaaagcaa cggtataaag tcgtttagtc gccgtaaaga tgctaatggg ttctctgttc
     5281 actctgtacg caccattcta aggtcagaga caacaatagg cacgttacca gcatcacaac
     5341 gtaatgaccg ccccgctata ccgaactact acgaaggtgt tgtagatata ccaacgttca
     5401 ataaagctca agagattctc gacaagaatc gtaaaggccg tacacctgca agtgacaacc
     5461 cactaacgat taacatcttc aaaggtctgt ttaggtgtca gtgtggggct agtgtccatc
     5521 ctaccggaac aaagaataag tatgctgggg tctacaggtg caataaccac ttagacggtc
     5581 gctgtgatgt tccaccgttg aagcgtaaac cgtttgaccg atggatgatt gataattttc
     5641 tggggatgat tgacgtgggg aatgatggag aatcagagag aaagattgca gcgttacagc
     5701 atgaggttga aattgtcaca gccagaatca agaaagctac cgccctactt cttgagatgg
     5761 atgatattga tgaactaaaa attcagctta aggaactgaa ccagaagcgc acagaactac
     5821 agaccacgat tgataacatg aggcgtaagg cttcactcac tgataaggaa ctaccccagc
     5881 ttaaagatat tgaccttatg actaaagcgg gtcgtgtcga gtgtcagttg attttgtcca
     5941 agcatctaaa agggcttaca ttgggtaagg attcggtaac tgtaacgcta cagaacgaca
     6001 ctgaaatcac catacctaca aacccgttac cgctaaatga tgggtcacca atctttgaaa
     6061 ttgctgataa agagctactg gatattgacg cttatcaact gtaattttca ggggctatga
     6121 agcccctttt tatgtctcaa taagtgccct tcttgttagg gttcacagag tactttcgca
     6181 gagcattacc cggcttaagc acctttgaag cttgctcatt agcggcatct gagaaggtgc
     6241 tctcaccatt tttaacgcta acgctcttag agcacgttac acgggctttt agcgcttcat
     6301 ctgataactc acttgccgaa tagccaaaaa gctctttaca ggtcagcata gggcttccaa
     6361 ggctcgttgt agtgtttccg gtgttctttt tactggtagc accgccatca ttcgtggtag
     6421 cggtctttcc agtagcagcc gaaacatcag tctgacctac gattgaacga aactctacac
     6481 cttggaaggt catctgaata cgaagaccgt tagcaatggt atgctcttta ctcgctttaa
     6541 actgagtaat gatactatca gtgatagcta ttccatctct acaaacaata gtgatgattt
     6601 gcttctggtc tcgccattgt tcaaccgtag aaataaaggt ttctacaagt tgcccctgtc
     6661 tggttaatag aactgaaccc gtcgaatcaa ccactacaac accgctaata gagatggtct
     6721 taggtgctct ctgaacatta tcagtgatag tttgccccga ttgcatagcc tgagttgtca
     6781 ccatcatcgg tgagtcatag tccatctgtt cagtagctgt taaggcaaga aaagattcca
     6841 cattatctct aaggtgaaaa tagataccat ctttacctga gtatctaatt tgctttgtgt
     6901 caaacattgt tgttattcct attctgttaa gcgtga
//
LOCUS       DAKISA010000166         6871 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.168, whole genome shotgun sequence.
ACCESSION   DAKISA010000166 DAKISA010000000
VERSION     DAKISA010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6871
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.168"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..539
                     /locus_tag="OVW45_004044"
     CDS             <1..539
                     /locus_tag="OVW45_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCV5528368.1"
                     /translation="IMYANGQYVEVDCKKAKEYLDKGVHIYGGPEDFLATCRKDMIDR
                     KTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGEVANLRVTFSIRRPSGKE
                     INQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFERKSCATVRTTIVAATAT
                     INGKDVELLKAGAIEQKW"
     gene            complement(595..1137)
                     /locus_tag="OVW45_004045"
     CDS             complement(595..1137)
                     /locus_tag="OVW45_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCV5528369.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(1121..1432)
                     /locus_tag="OVW45_004046"
     CDS             complement(1121..1432)
                     /locus_tag="OVW45_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCV5528370.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            complement(1617..2507)
                     /gene="cyoE"
                     /locus_tag="OVW45_004047"
     CDS             complement(1617..2507)
                     /gene="cyoE"
                     /locus_tag="OVW45_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCV5528371.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGFAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(2519..2848)
                     /locus_tag="OVW45_004048"
     CDS             complement(2519..2848)
                     /locus_tag="OVW45_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCV5528372.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(2848..3462)
                     /locus_tag="OVW45_004049"
     CDS             complement(2848..3462)
                     /locus_tag="OVW45_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCV5528373.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(3452..5443)
                     /gene="cyoB"
                     /locus_tag="OVW45_004050"
     CDS             complement(3452..5443)
                     /gene="cyoB"
                     /locus_tag="OVW45_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HCV5528374.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(5465..6412)
                     /gene="cyoA"
                     /locus_tag="OVW45_004051"
     CDS             complement(5465..6412)
                     /gene="cyoA"
                     /locus_tag="OVW45_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HCV5528375.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
BASE COUNT         1895 a         1718 c         1681 g         1577 t
ORIGIN      
        1 gcattatgta cgccaatggt cagtatgttg aggtagattg taagaaagcg aaggaatatc
       61 ttgataaagg tgtccacatt tatggtggcc cggaagactt tctggctact tgtcgaaaag
      121 acatgattga cagaaaaacg gttgacgata cgttacccgt gattacggtt acccgttctg
      181 ggatgcgaga taatttttta gataaaggtt tttcctgcat ggatagcctc ttcgccacca
      241 ccaataaatt aggcgaagtg gccaatcttc gcgtgacatt cagcattcgt cgcccatccg
      301 gtaaggagat taaccaaacg gttggcttcg cgcctttcgg tttaaaccgg ctgaatatta
      361 gctttactga ttacctattt ggttcattta ccagtaattc gtctcttatt ctttataaac
      421 cggaatttga gcgaaaatct tgtgcaactg taaggaccac aatcgttgcc gcaacggcaa
      481 ccattaacgg caaggatgtg gagttgctga aagcgggggc aattgaacaa aagtggtaac
      541 gctgctcagc ccccggctaa ggcaaaactt gccctggcgg gtggcgataa taatctaagc
      601 cgcgagaata tgctgaatcg ccaaaaacat aggtacagca ccaaaagcat ttggtgtcgc
      661 tgaaagctga acaaagccga gacgagcata aaaattaatg gcggcagggt cagcatcaag
      721 ataaacccct ttaattggta atgcctggtg aattattttt acatgttcaa aaaaatcaca
      781 tagtaggtcc tgaccaaagc cccgcttttg atacttccgt gctaccccca acatgactaa
      841 tctgacaaca ccaatttctg aaggttgtga accctgaagg acgccagaaa cgcgttgttt
      901 ttccagcgaa tatgccgtaa aagtacagat gccgatcagt tcaccacttt gtctgtcaat
      961 aagtgcttta gccgcacagt cgctattacg cacacttttc tttagcgatg cgcgtacaaa
     1021 tttatcgata acagggttac cgcaatcaaa ctgtttttgc ccaggataag taatatcaga
     1081 ctgataatta caggagagga ctatgggcag agtaatctct tcatgcttgt cgaccatcgc
     1141 tgtttttcct tttcattaac gccttgaggg cgagcgtcgg ttgagcaggt tcagcgatga
     1201 gttggttcat cagttcccag ctttgattcg aaagctcacg gcgacgttgg ttatcaagca
     1261 cactttcggc gcgttccata gcagcgctca aaataaaagc gcttaaatct accccaacgg
     1321 cttgcgcagc ttcacgaagt ttgtttttgg cgtcgggact agttttgagt tcgacccgcg
     1381 catttttcag cgttttatcg acatcgggta gagtggctgt ttttccaggc attatgacct
     1441 ctttgattaa ctgaatgaaa tcgtagggag tcgatctcat acataatacg gctatcaacc
     1501 gtatgattaa tttatgttca tctgctcact ctgtcaagac atacggttat tagccgtatc
     1561 tctgatctca gacaataaaa aaggtgccgt agcacctttt taatagagag gttgtgttac
     1621 caaacagcag ccagcagcgt atgcgagtcc ggcaccataa aatcaacgga catcatcacc
     1681 gagagggctg tgatggcgat gatagagaag ccgaacagct tgcgcgccca gatcctgtca
     1741 tcagcaactt tataaccgcg cagggccata cctaaccacc agacgctcac cgccgcggcg
     1801 accaccagat atttataccc agcgtaaccg ccaagagaga gcatcagcgt ggcaacggca
     1861 aaggcgatga tatacagcgt gatgtgattc ttcgccaccg aaatgccttt taccactggc
     1921 aataccggaa tgtttgccgc ctgataatcc ttaaagcgga aaatggcgat ggcataggag
     1981 tgaggcatct gccacaggct gaagatagcc agcaggatcg ctgcgccgct atcgaactcg
     2041 ccggttactg cacagtagcc aatcaccggc ggcgcagcgc cggagagcga accaatcaat
     2101 gtgccgtaga ccgagtggcg tttcatgtac aggctataaa cgccgacata aaccacaaag
     2161 cccatcaccc ccagccagca ggccagcgga ttcgcgccaa accacagcag cataaagcca
     2221 gcaaagccta gcaaggtggc gtacaccagc gagacagcag gagagatcag gcctttaacc
     2281 agcacccgat tcttcgtcct ttccatcttt ctgtcgatat ccctgtcgat gtagttgtta
     2341 aacacacaac ccgacgccac aaccagtgac accccaacca gcgtgtagat aaacagggga
     2401 taatcaatgc tgccctttga ggccagcagg aatcccccaa tcaccgagat caggttgcca
     2461 aagatgatgc ctggtttcgt tacttgcagg tattgcttaa acatcataac cgccgctctt
     2521 agtgcatcat catattgtag ttgaggttcc acataatcca gatggagcct acaaccagga
     2581 tagcgatgat tagcacggtg aagacaaacg ccgtcatgtt ccagccttca tctgatttgg
     2641 tattcatgtg caggaagcac accagatgca ccagaacctg taccactgcc attgccagga
     2701 ttgttcccag aattacggcc ggagaggcag ctcctgtcat caccatccag aacggaatca
     2761 ccgtcagaat gatcgacagg ataaagcctg tcatgtaggt ttttacgctg ccatgggacg
     2821 cgccgctgtg atcggtagaa tgactcatta catcgccccc atcagataaa caacagtgaa
     2881 cacacagatc caaaccacat ccaggaagtg ccagaacagg ctcaggcaca tgatgcgggt
     2941 acggttagtg ctggtcaggc cgcgacgagc gatttgtacc atcagcaccg ccatccagat
     3001 aagaccggaa gtgacgtgca gaccgtgcgt gccgaccagc gcaaagaacg ctgacaggaa
     3061 gccgctgcga tccggaccca tgccgttaac aatcaggtga tggaattcgt agatttccat
     3121 cccgataaat ccggcaccaa acaaccaggt caacgccagc caggagataa cctggctttt
     3181 gttgtttttg tacatggcga tagccgccat gccgtaggtg atggagctga acaacagcaa
     3241 gaaagtttca accagaacga acggcagttc gaaaatgtcc ttacctgtcg ggccgcctgc
     3301 ggtgccgttc accagaacgg cataggtagc aaacaagatt gagaacagaa tgcagtcgct
     3361 catcaggtag atccaaaatc cgaagatttt ggttccgcct gcatcgtggt gcccgtgttc
     3421 gtgcgcgtgg gcagtcgcgt gcgtcaaagt atcagttgcc atttttcagc cctgccttag
     3481 taatctcatc gaaatgctgg ttttccagtt tttcgatttc tgccaccggc acgtagtaat
     3541 ccacgtcctc gtcgaagctt ttcacgatcc aggtgatgat catgcctgcg aagccaacaa
     3601 tcgccagcca ccagatatgc cagatcatgg cgaaaccgaa gatggtggag aaagctgcaa
     3661 tgacgatacc tgcaccgctg tttttcggca tatgaatttc ttcatagtgg tcaggctttt
     3721 tatacgcttc gcctttctct ttcatttccc agaatgcatc acgctcgtga acgtgcggca
     3781 caacggcaaa gttatagaac ggaggcgggg aagaggttgc ccactccagc gtacggccac
     3841 cccacgggtc gccagtcagg tcacggttct ggtcgcggtc gcgaatagaa acgtacatct
     3901 gaataacgag gcagagaata cccagcgcaa tcagtactgc accgctagct gcaatcatca
     3961 gcatggtgtg gaactgcggg tcaatctgct ggctcaaacg acgggtcatg cccatgaagc
     4021 ccagcgcata cagcggcata aaggcaacga agaagccgat gatccagaac cagaacgcgc
     4081 gtttacccca ggtttcattc agtttgaaac cgaacgcttt aggccaccag taggtcatcc
     4141 ctgcgaagca gccgaagacc acgccgccga tgataacgtt atggaagtgc gcaatcagga
     4201 acaggctgtt atgcagaacg aagtccgcgc ccggtactgc cagcagcacg ccagtcatcc
     4261 cgcccaccga gaaagtgacg ataaaaccga tggtccacag catcgcagaa tggaacacga
     4321 tgcggccctg atacatggtg aacagccagt tgaagatctt caccccggtc gggatggcga
     4381 taatcattgt ggtgatacca aagaaggcgt ttacgttcgc gcccgcaccc atcgtaaaga
     4441 agtggtgcag ccaaacgatg aacgacagca cggtgataca gacggttgcc cataccagcg
     4501 aggtataacc aaacagacgt ttacgcgaga aggttgccgc aatttcggag aacacaccga
     4561 aaacaggcag gatcaggatg taaacttccg ggtggcccca ggcccaaatc aggttgatgt
     4621 acatcatcat gttgccaccc atatcgttgg taaagaaatg ggtgcccaga tagcgatcca
     4681 gggtcaacaa cgcgacggta accgtcagaa ttgggaagga agcaataatc agtacgttcg
     4741 cgcacagtga tgcccaggta aatactggca tcttgaacat ggtcatgccc ggtgcgcgca
     4801 tcttcagaat ggtaacgaag aagttgatac cggtaagcgt cgtacctata ccggatagct
     4861 ggagactcca tatccagtaa tcgacaccga ctcccggact gtactctatt cccgatagcg
     4921 gtggataggc cagccagccg gtctgcgcaa attcgcccac gccgagagaa acgttaacca
     4981 gaatcacacc aacaacagta aaccagaagc ttaagttgtt gaggaacggg aacgcaacgt
     5041 cacgcgcgcc gatctgcagc ggaaccacca ggttcatcag accgataacg aaaggcatcg
     5101 ccacgaagaa gatcataatc acgccgtgcg cggtgaagat ctgatcgtag tggtgaggtg
     5161 gcaggaagcc cgcttcgccc gccgaggcaa gagcctgctg gctacgcatc ataatggcgt
     5221 cagcaaaacc acgcagcaac atcacaatcg ccacgatgat atacatgata ccgaggcgtt
     5281 tatggtcgac ggaggtcagc cactctttcc acaggtaggt ccacttaccg aagtaagtga
     5341 tcaggccaac gagcgccaga cctcccaaaa taatgccagc gatcgtaacc atgacgatag
     5401 gttcatggaa cgggactgca tcaagtgata attttccgaa catctttatt cttcctcaac
     5461 ccctttaatg ggcggattcc gcgtggctca tgtccatgcc ttccatacct tcgtgtgcac
     5521 tgtgctcacc ttctggctgg gtcatgtcca tgctcttacc gtgagccata aacttgttaa
     5581 tcacatcggc aaacaagtct ggtttcacgt tggagaaata ttccacctgg ttgtattcgc
     5641 taggcgcggc cagtttttcg aacgcagcca tgtcagacat ggtgttcggc gactgtttcg
     5701 cttttgcgac ccattggtcg aattcggcgc gatccggtgt tgcaatagct ttgaacttca
     5761 tgcctgagaa gcccggcccg ctgtagctgg cggagatacc gtcataagta ccgggttcgt
     5821 tggcgatcag atgcaggcga gtctgcatac cggccatggc ataaatctgg ctacccagac
     5881 gcggaatgaa gaaggagttc atcacggagt tggaggtcac tttgaagtac accggagtgt
     5941 tcgccgggaa agcgatttca ttcacggttg caatgccctg ttccgggtag atgaagaacc
     6001 atttccagtc catggaaacc acttcgatgg taatgggctt ctcgtcgtgt gccagcggct
     6061 tgctaggctc aagagcgtga gtggttttcc aggtcagtac cgcaaggaag atgatgatta
     6121 agataggtac cgtccagacc acagcttcca ctttattgga gtgtgaccag ttcgggctgt
     6181 acttagcatc tttattgctc gcacgatact tccaggcgaa accaacagcc atcaagattg
     6241 cgggaataac gacaatcaac atcaggccaa atgccgtcag tatcagtgaa cgttgctcca
     6301 gaccaatctg tcctttggga tctaacagcg cagaattaca gccactgagc aatacagtgc
     6361 ctgcaaataa tgacaaccat cccaaacttt tattgtattt cctgagtctc atttaacgac
     6421 ctcaattcca cgggatctgg tggcgtttaa agtgtgtggg cattttacgg gaaggttaca
     6481 ttactgtaaa catgattaaa ttcctgttac ttggatttgg ctgtcaggtt acatatgttg
     6541 caaaacatat caagttgatt ttaagaagct gttgcaaaag ggcgttataa cgaaagggga
     6601 gtgcagaagg agtttggacg ggcaattggt ataaccaatc tggaataaaa caattgttta
     6661 acaaataatt atcattatgg tgacaattgg gtggggggga aaataaagac tcattagctg
     6721 aatcgtgtaa gaaaaaatag aattcataaa ttgcgtcaat aatttccaaa cgctaagccg
     6781 cacaaaagaa caaatattat taagcgatac tgatgtttat ttagccgtaa taattacggc
     6841 gagtgattac tacagctaaa taatatttac a
//
LOCUS       DAKISA010000167         6494 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.170, whole genome shotgun sequence.
ACCESSION   DAKISA010000167 DAKISA010000000
VERSION     DAKISA010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6494
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.170"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..164)
                     /locus_tag="OVW45_004052"
     CDS             complement(<1..164)
                     /locus_tag="OVW45_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCV5528376.1"
                     /translation="MARYGLPDEAWTIIKPLLPPEPATPRAGRPWAEHRKIINGMFWV
                     LCSGAPWRDLP"
     gene            complement(364..870)
                     /locus_tag="OVW45_004053"
     CDS             complement(364..870)
                     /locus_tag="OVW45_004053"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528377.1"
                     /translation="MNFTKHALTIGLIALSFNALAHKSTVDVATASVDTSFIIQADCA
                     ISVIAKAPKTFTLSQVKAQARAADIIITPTCGGKQLWAEMKEVDSKGFGIARSDAGDL
                     ASVTWVQDGNWDYAESNIEKVAKTTKKTVASQEVPYPASFSAGGEYGLPKKVGKYKFG
                     LTVGYWVD"
     gene            complement(1198..1668)
                     /locus_tag="OVW45_004054"
     CDS             complement(1198..1668)
                     /locus_tag="OVW45_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_110996818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCV5528378.1"
                     /translation="MHVTGGAEVKKNGKTHVLLGGLAFVMMSGFSQAAELSLDVRKSA
                     GSELRDGEKIATGRIICREAHTGFYIRINGREDESRLGHWLIQGKHDGRNELRVRVDG
                     EGWSVVTEGEHWLVKAGTEEQAVFDVVADGKQHAGTDEYILSVSGNCADNRSPE"
     gene            complement(1659..4241)
                     /locus_tag="OVW45_004055"
     CDS             complement(1659..4241)
                     /locus_tag="OVW45_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCV5528379.1"
                     /translation="MRKFTCIPKYTKTRVALAVMLACAFIYSSTGEARRYSFDVSQLT
                     GGGKGVDLTLFEEGAQLPGIYPVDVILNGTRVDSREMAFHIERDRDGSPYLKTCLTRD
                     MLARYGVKVEEYPGLFRQGEGGRSKDAEACADLSAIPGATETHQFAAQQLLLGIPQVA
                     LRPQFTGIAPEASWDDGIPAFLLNWQANAYRSEYRGYGKSVSDSFQATLEPGINVGPW
                     RVRNLTAWNKLSGQAGKWESSYTRAERGLNGIKSRLTLAESYTPSDVFDSVSFRGVML
                     GSDEDMVPYSQREFAPVVRGIARTQAKIEVRQNGYLIRSQTVAPGAFSLADLPVTGSG
                     GDLVVTVLESDGTTQVFTVPFTTPAIALREGYMKYNITGGQYRSSDDEVEHTSLGQAT
                     LMYGLPWGLTAFGGFQGAEHYQSAALGLGWSLGRLGAVSVDGIHSRGQKKEREQESGN
                     TWRVRYNKSFEMTGTSFTAASYQYSSDGYNTLSDVLDTWRGRGFRSYSNSDNRSRRTT
                     LNLSQSMGRLGYVSLWGSRDEYRDGRGNRDSMGASYSTTWKDVSLSVNWSRNRNAGGY
                     YGWRQGKTEDSVSVWMSVPLGRWLGNTDNDIRATAQVQHNTGQDTRYEAGMNGRAFDR
                     RLSWDVREQVTPGSETNRDSSRLNLTWFGTYVEATGMYSYSSHSRQMSAGLSGGLVAH
                     RHGVTLGQKTGETVALVEAPGVSGAFVSGWSGVRTDSRGYTLSGWVTPYQENVVALDP
                     TTFPADAEVPQTDCRVVPTKGAVVSAGFTTRVGGRALVTLTRGDGTPLPFGAVVTVDG
                     ESGRTSGAAGVVGDNGEVYMSGLSETGKLKAQWGQSSQCYADYRLPEEKGPAGIFLTR
                     SVCM"
     gene            complement(4401..5078)
                     /locus_tag="OVW45_004056"
     CDS             complement(4401..5078)
                     /locus_tag="OVW45_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCV5528380.1"
                     /translation="MDLSSKVFSLHLGATRVVYNPDLSSEKLTVINDHDYPILVQSDV
                     LAEDQKSRAPFVVTPPLFRLDALQSSRLRIVRTGGSFPGDHESLQWVCVKGIPPKEGD
                     KWAEEANKSKLADKVSLQVQLSVGSCIKLFVRPSSVKGHPEDVAGNVTWQKTGNKLKG
                     NNPTPFFINLSELKMGGKEVKERHYIAPFSSYEYPLPVGATGKVQWQVITDYGGKSKL
                     FESETSL"
     gene            complement(5907..6192)
                     /locus_tag="OVW45_004057"
                     /pseudo
     CDS             complement(5907..6192)
                     /locus_tag="OVW45_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(6340..>6494)
                     /locus_tag="OVW45_004058"
     CDS             complement(6340..>6494)
                     /locus_tag="OVW45_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCV5528381.1"
                     /translation="RGMYQQLLKVDPLKCELCGGQMRFTGLKMGYRLAEQVMMHEPLA
                     RMRWCG"
BASE COUNT         1703 a         1882 c         1295 g         1614 t
ORIGIN      
        1 ggtaaatcgc gccatggtgc accggaacat aacacccaga acatgccatt gatgatttta
       61 cggtgctcag cccatgggcg tccggcccgt ggtgtggcgg gttcaggagg taacaggggc
      121 ttgatgatgg tccatgcctc atcgggaagg ccgtagcgag ccatagttca atatgttgtg
      181 taaacagaca gttactatag ctcagatgat taagggacac agcctaacag aagagtcact
      241 ttcgcactgt tggtaacaat cacgatacga ttttgcactt gactgcatcc ctgcataatg
      301 aacttccttg aaagttaatc aatcagtatg ctttgtagag aacactaatt tttgcggtct
      361 aactcagtcc acccaatagc caacagtgag gccaaatttg tattttccaa ctttcttcgg
      421 taacccgtac tcccctccag ctgagaagct tgcaggataa ggaacctctt gagaagccac
      481 ggtctttttg gttgtcttag ccactttctc aatattactt tcagcataat cccaattccc
      541 atcctgtacc caagtaacag aagcaagatc accagcatca cttctcgcaa taccaaatcc
      601 cttagagtca acctctttca tttctgccca taattgttta ccaccacatg taggtgtaat
      661 gataatatct gctgctctag cttgagcttt gacttgactc aaggtaaacg tttttggagc
      721 ctttgcaata acagaaatcg cacagtcggc ctggatgata aaggatgtat caacagaagc
      781 cgttgcaaca tcaacagttg atttatgagc aagagcatta aaggacagcg caataagtcc
      841 gatggttaaa gcatgcttag taaaattcat atacagaatc tccattattt gctaaatatt
      901 attaccagta aataacgacc tcacgggtgg ccccgtgttc atcaaactgt gccccagcca
      961 gtccattccg tgcgagattt ttccgatata cgttttgtcc ggatgttgtt taaaccctcc
     1021 ccagtcgaga aattcattga gcagggcaac actctgccgt aatcgccacc gtgttctggt
     1081 aagcagaata aaattatcca tatcgcgcat aataaatatc ctctgtcgcg ttaaattcag
     1141 catcaatgtg ataatttacg gatttaaccg ttttattttc tgtcatgact tgcgcaatca
     1201 ttccggtgaa cgattatccg cgcagtttcc actgaccgaa agaatatact cgtccgtacc
     1261 tgcatgctgt ttgccgtcag ccaccacatc aaagacggcc tgctcttccg tgccggcttt
     1321 aaccagccag tgctctcctt ctgtcaccac tgaccaacct tcaccgtcca ctctgacccg
     1381 gagttcattc cgaccgtcat gttttccctg tatcagccag tgccccaagc gactttcatc
     1441 ctcacgacca tttatccgga tataaaaccc cgtatgtgcc tcacggcaga taatccggcc
     1501 tgtcgcaatt ttctccccgt cccgtaactc agaccctgca gatttacgaa catcaagact
     1561 cagctctgct gcctgtgaaa aacctgacat catcacaaaa gccagcccac cgagcagcac
     1621 atgcgttttt ccgttctttt tcacttctgc ccctcccgtc acatgcatac actgcgggtc
     1681 aggaatatcc ccgccggacc tttctcttcc ggcagacggt aatccgcata acactgactg
     1741 ctctgccccc actgtgcctt cagtttcccg gtttcagaca gaccgctcat gtacacctca
     1801 ccgttatcgc ccaccacacc ggcagcacct gatgtacgtc ccgattcacc atccaccgtc
     1861 actaccgcac caaacggcag cggcgtaccg tcaccccggg tcagcgtcac taatgccctg
     1921 ccaccaacgc gggtggtaaa tcctgcactg accaccgcac ctttcgtcgg tacaacccgg
     1981 cagtctgtct gtggcacctc cgcatccgcc gggaacgttg tcgggtccag tgcgacaaca
     2041 ttttcctggt acggggttac ccagcccgac agggtgtagc cgcgggaatc cgtcctgaca
     2101 cctgaccagc cactcacgaa agcaccactc acccccggtg cctcaaccag tgccactgtc
     2161 tcaccagtct tctgtcccag cgtcacacca tgacggtgag caaccagtcc gccggacagg
     2221 cctgcactca tttgtcttga atgactactg taactgtaca ttcccgtcgc ttcgacgtat
     2281 gtcccgaacc aggtcagatt aagtcgactg ctgtctcggt ttgtctcact tcccggtgtc
     2341 acctgttcac ggacatccca ggacagacga cggtcaaacg cccgaccgtt catcccggct
     2401 tcataacgcg tgtcctgacc tgtgttgtgc tgcacctgtg ccgtggcccg gatatcgtta
     2461 tccgtattcc ccagccagcg ccccagcgga acactcatcc acacgctgac actgtcttct
     2521 gtttttccct gccgccatcc gtagtagccg cccgcgttgc ggttccgtga ccagttaact
     2581 gacagggaga catctttcca ggttgtgctg taagatgccc ccatggagtc acggtttccc
     2641 ctgccgtcac ggtactcatc acgactgccc cacagactca cataccccag tcgccccatg
     2701 gactggctca ggttcagtgt ggtccggcga ctcctgttat cactgttact gtatgaacgg
     2761 aaacctctgc cacgccaggt atccagcaca tcagagagcg tgttataccc gtcagaagag
     2821 tactgataac tcgccgctgt aaagctggtc cccgtcatct caaaggattt gttataacgg
     2881 acacgccagg tattcccact ttcctgttca cgctcctttt tctgaccacg ggaatgaata
     2941 ccgtccacag acaccgcccc cagtctgccc agcgaccatc ccagccccag cgcagcagac
     3001 tggtaatgct cagctccctg aaagccaccg aatgccgtca gcccccacgg cagcccatac
     3061 atgagcgttg cctgccctag tgacgtatgc tcaacctcat catcagaaga gcggtactgc
     3121 ccgccggtaa tgttgtactt catgtatccc tcacgcaggg caatggccgg cgtggtgaac
     3181 ggcaccgtaa acacctgcgt cgtcccgtca gactccagta ccgtcaccac caggtcgcca
     3241 ccggaaccgg tcaccggcag gtctgccaga gaaaatgccc cgggtgccac tgtctgactg
     3301 cgaataaggt aaccattctg acgcacctca attttcgcct gagtacgggc aataccccgc
     3361 accaccggcg caaactctcg ctgactgtac ggcaccatgt cctcatcaga acccagcatc
     3421 actccccgga acgacacgct gtcaaaaacg tctgacggag tgtaactctc tgccagagtc
     3481 agacgactct ttatcccgtt caacccacgt tcagcacggg tgtatgaact ttcccacttt
     3541 ccggcctgcc ccgataattt attccaagcg gtcagattcc ggacacgcca tggtcccacg
     3601 ttaatacccg gttccagcgt agcctgaaac gaatctgaaa cacttttccc gtaaccacgg
     3661 tactctgaac gatacgcatt tgcctgccag ttcagcagaa aagctggtat accatcatcc
     3721 cacgacgctt caggtgcaat gcctgtaaac tgaggacgta atgccacctg aggaatgccc
     3781 agcagtaact gttgggccgc aaactggtgt gtttccgtag caccaggaat ggctgacagg
     3841 tctgcacagg cctctgcatc cttactacgg cccccctccc cctgacggaa cagtcccgga
     3901 tactcctcca ctttcacccc gtaacgagcc agcatatccc gggtcagaca ggttttcaga
     3961 taaggagaac cgtcacggtc acgctcaata tgaaacgcca tttcacgcga atccacacga
     4021 gtcccgttca ggataacatc taccggataa atacccggaa gctgtgcgcc ttcctcaaag
     4081 agggtaaggt caacaccctt tcctcccccg gtcaattgag aaacatcaaa agaatacctc
     4141 cgggcttctc ctgtactgct gtaaataaaa gcacaggcaa gcatgacagc cagagccact
     4201 ctggttttcg tatatttcgg aatacaagtg aatttgcgca tggtatttct gcctggcaat
     4261 atcccttcca tttaatctgc acgattattg caatccctgc cctgtgacag agcgagggaa
     4321 ctgaataata atcccgtcac ggaaaataaa attaatataa gtgatatatt gcggttttaa
     4381 tgcatcagat aacaacctca tcagaggctt gtttctgatt caaacagctt actctttcca
     4441 ccgtaatcgg ttatcacctg ccactgaact ttccctgttg ctccaacagg tagtggatat
     4501 tcgtaagaag agaatggcgc aatataatga cgttccttca cttcctttcc acccattttt
     4561 agttccgaca gattgataaa aaaaggcgtc ggattattac ccttcagctt atttccagtt
     4621 ttctgccatg tcacattccc cgctacatct tccggatgcc ctttcaccga cgacggacga
     4681 acaaagagtt tgatgcaact gccgacagac aactgcacct gcagtgatac cttgtcagct
     4741 aacttacttt tattcgcctc ttctgcccat ttatcgcctt ccttcggcgg aatgcctttc
     4801 acgcaaaccc actgcagact ctcatggtct cccggaaagc tcccgcctgt acgaacaatc
     4861 cgcagacgac tggactgaag tgcatcaaga cggaataacg gcggcgtcac cacaaaaggt
     4921 gcgcgactct tctggtcttc tgccagcaca tccgactgca ccaatatggg ataatcatgg
     4981 tcattaatga ctgtcagttt ctcactggat aaatccggat tgtaaacaac acgcgtcgcc
     5041 cccagatgca aggaaaatac ctttgaagaa aggtccatgt ttttttcctt tcctgaatcc
     5101 aatacatccg cagcaaaaga aaaattaaca cttccgctca gcaaaaaccc agccaacaca
     5161 tacaacaccg gacgatgcaa acttactgtt aaataatcac cgcacatctg ataacacctc
     5221 aacccgactc tgaccaaaca cagaagttat acagtcctcg caaattctac tgccgctggt
     5281 ataataagaa agcaccatag cgttcaacta atttactcgc gccagtcgag aaagactcac
     5341 gctaaaataa ctattattaa gccattaagc cattaagcca ttaagccatt aagccattaa
     5401 gctattaagc tattaagcta ttaagctatt aagctattaa gctattaagc tattaagcat
     5461 ctaacgatgc acacacatcc ctgcgtttac ccccgtatat ttaacactca ctaatttaga
     5521 gttaataact cacaccccat ttttatgaaa attacataaa ataatattag aaaaaaaata
     5581 aacaaatatc cacccgttaa aaacaaaaaa accaaacagt accaaaatat gaaccatttt
     5641 tactatttat acttgcgttg atttggcgtt tacctgcgat gcaattatat aacaattgat
     5701 taatgctttg attaattaac atttacatac attaataatg taactctatt gaggtataat
     5761 atataatacg ggagttgaca tgggatcccg tatatcagca gttattttta ctcggcagtt
     5821 cattgattat tatcaataat aaacgtaaac cacgtggccg aaatattgat gggataacac
     5881 caagaggaaa agggtgatgg ctccaggaac atatgttttt cgataaaagc accgataact
     5941 ttttcatgaa attttacaga gcgagagaaa cagatggttc tgcgagccag gcgtttgatt
     6001 cgggttctca ggggcagatt gttgcgctca atgcgctgag taaaaatctt accagtcaga
     6061 tacttctcct tcggtaactc tctggtgtaa ctaccccatt cgtcagtggt caacataccg
     6121 atggtaaaag ggggaagcag cgccagcagt tcatcgacag gaacttaaat ccccaaaaga
     6181 ttagaaaagc accgaagaat gcgcgtttat tcaacacgac ctgatcatca cgtaattggt
     6241 atagacactt gatattgctt acattgattg attcaatata tctctgatgg tttttgctcc
     6301 gttcccctca acatggacgc aacttcccct ctgcggcgct cagccgcacc accgcattcg
     6361 tgccagcggc tcatgcatca taacctgctc tgccagacgg taacccatct tcagtcccgt
     6421 aaaccgcatc tgacctccgc acagctcgca cttcagcggg tcaaccttca gtaactgctg
     6481 atacatccct ctcc
//
LOCUS       DAKISA010000168         6394 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.171, whole genome shotgun sequence.
ACCESSION   DAKISA010000168 DAKISA010000000
VERSION     DAKISA010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6394
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.171"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            6..326
                     /locus_tag="OVW45_004059"
     CDS             6..326
                     /locus_tag="OVW45_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001409844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine transporter"
                     /protein_id="HCV5528382.1"
                     /translation="MTMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASY
                     RYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKD
                     SQRA"
     gene            428..1882
                     /gene="amn"
                     /locus_tag="OVW45_004060"
     CDS             428..1882
                     /gene="amn"
                     /locus_tag="OVW45_004060"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCV5528383.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWNLITADAQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTIDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2225..2941
                     /locus_tag="OVW45_004061"
     CDS             2225..2941
                     /locus_tag="OVW45_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCV5528384.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNAGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(3393..3468)
                     /locus_tag="OVW45_004062"
     tRNA            complement(3393..3468)
                     /locus_tag="OVW45_004062"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3433..3435),aa:Asn,seq:gtt)
     gene            complement(3569..5212)
                     /gene="yeeO"
                     /locus_tag="OVW45_004063"
     CDS             complement(3569..5212)
                     /gene="yeeO"
                     /locus_tag="OVW45_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HCV5528385.1"
                     /translation="MLRHILTAKNLLSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            5217..5292
                     /locus_tag="OVW45_004064"
     tRNA            5217..5292
                     /locus_tag="OVW45_004064"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5250..5252,aa:Asn,seq:gtt)
     gene            complement(5330..6280)
                     /gene="cbl"
                     /locus_tag="OVW45_004065"
     CDS             complement(5330..6280)
                     /gene="cbl"
                     /locus_tag="OVW45_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCV5528386.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
BASE COUNT         1821 a         1542 c         1371 g         1660 t
ORIGIN      
        1 gagtcattac catggcgctt gaagcacaat ctattttctg gatagttttc ttctccataa
       61 tgctggcaaa cattgcgcat gacatggtgg tgtgtgtgca acaaccgatg tttaccgaaa
      121 tgtttggtgc cagttatcgc tatagtggcg ctggagtcgg ttaccaggtt gccagtgtgg
      181 ttggcggtgg atttacacct tttattgccg ctgcactcat cacttacttt gccgggaact
      241 ggcatagcgt cgccatttat ttgctggctg gatgcctgat ttccgcaatg accgctttgt
      301 tgatgaaaga cagtcaacgc gcttgatagc ctggcgaaga tcatccgatc ttcgccttac
      361 acttttgttt cacatttctg tgacatacta tcggatgtgc ggtaattgta tggaacagga
      421 gacacacatg aataataagg gctccggtct gaccccagct caggcactgg ataaactcga
      481 cgcgctgtat gagcaatctg tagtcgcatt acgcaacgcc attggcaact atattacaag
      541 tggcgaatta cctgatgaaa acgcccgcaa acaaggtctt tttgtctatc catcactgac
      601 cgtaacctgg gacggtagca caaccaatcc ccccaaaacg cgcgcatttg gtcgttttac
      661 tcacgcaggc agctacacca ccacgattac tcgccctact ctctttcgtt cgtatcttaa
      721 tgaacaactt acgttgctgt atcaggatta tggtgcgcat atctctgtgc aaccctcgca
      781 gcatgaaatc ccttatcctt atgtcatcga tggctctgaa ttgacacttg atcgctcaat
      841 gagcgctggg ttaactcgct acttcccgac aacagaactg gcgcaaattg gcgatgaaac
      901 tgcagacggc atttatcatc caacggaatt ctccccgcta tcgcattttg atgcgcgccg
      961 cgtcgatttt tccctcgcac ggttgcgcca ttataccggt acgccagttg aacattttca
     1021 gccgttcgtc ttgtttacca actacacacg ttatgtggat gaattcgttc gttggggatg
     1081 cagccagatc ctcgatcctg atagtcctta cattgccctt tcttgtgctg gcgggaactg
     1141 gatcaccgcc gaaaccgaag cgccagaaga agccatttcc gaccttgcat ggaaaaaaca
     1201 ccagatgcca gcatggaatt taattaccgc cgatgctcag ggtattactc tggtgaatat
     1261 tggcgtggga ccatcaaatg ctaaaaccat ctgcgatcat ctggcagtgc tacgcccgga
     1321 tgtctggttg atgattggtc actgtggcgg attacgtgaa agtcaggcca ttggcgatta
     1381 tgtacttgca cacgcttatt tacgcgatga ccacgttctt gatgcggttc tgccgcccga
     1441 tattcctatt ccgagcattg ctgaagtgca acgtgcgctt tatgacgcca ccaagctggt
     1501 gagcggcagg cccggtgagg aagtcaaaca gcggctacgt actggtactg tggtaaccat
     1561 agatgacagg aactgggaat tacgttactc agcttctgca cttcgtttta acttaagccg
     1621 ggccgtagca attgatatgg aaagtgcaac cattgccgcg caaggatatc gtttccgcgt
     1681 gccatacggg acactactgt gtgtttcaga taaaccatta catggtgaga ttaaacttcc
     1741 cggtcaggct aaccgttttt atgaaggcgc tatttccgaa catctgcaaa ttggcattcg
     1801 ggcgatcgat ttgctgcgcg cagaaggcga ccgactgcat tcgcgtaaat tacgaacctt
     1861 taatgagccg ccgttccgat aataagttct gaaccttctt atcagaaata gatgtgaagg
     1921 agtaagtaag acacctggca aatagcctgc aataatcgtg ggctgtttgc ttccttgggc
     1981 ggatacgagt tttattatcg tcttaatgat ttccacatat taaaagcaag tatgctttca
     2041 aaacacaatt ataaaaaatc ccgccaacaa tataagtttt tataaaatta aatataaaat
     2101 tatggcttta gaatattttt atttctaata gacgagattt ttcctgttat gatataatat
     2161 gccgaattaa cacatgttaa cgatttacca gtaatgtaaa taaattttcg aggagatcat
     2221 tccagtggga cgtaaatggg ccaatattgt tgctaaaaaa acggctaaag acggtgcaac
     2281 gtctaaaatt tatgcaaaat tcggtgtaga aatctatgct gctgctaaac aaggtgaacc
     2341 cgatccagaa ttaaacacat ctttaaaatt cgttattgaa cgtgcaaagc aggcacaagt
     2401 tccaaagcac gttattgata aagcaattga taaagccaaa ggcggcggag atgaaacgtt
     2461 cgtgcaggga cgttatgaag gctttggtcc taatggctca atgattatcg ccgagacatt
     2521 gacttcaaat gttaaccgta cgattgctaa cgttcgcaca attttcaata aaaaaggcgg
     2581 caatatcgga gcggcaggtt ctgtcagcta tatgtttgac aatgctggtg tgattgtatt
     2641 taaaggatca gaccctgacc atatttttga aattttactt gaagctgaag ttgatgttcg
     2701 tgatgtgact gaagaagaag gtaacattgt tatttatacc gaacctactg accttcataa
     2761 aggaatcgcg gctctaaaag cagctggaat cactgagttc tcaacaacag aattagaaat
     2821 gattgctcaa tctgaagttg agctttcccc agaagattta gaaatctttg aagggcttgt
     2881 tgatgccctt gaagatgacg acgatgtaca aaaagtttat cataacgtcg caaatctcta
     2941 attatctttt aaagaaatct gtctttacgg cagatttctt taatctcata taattcttat
     3001 aaaaatataa gattcaactc gtcatattga ttataccccc ccgttcccag agaaataata
     3061 tttattaaaa ttccagttct tctttttctg attacagaag gcaaagtggc aattacgcat
     3121 agtttcccga taaagacgcg atagcgacat cccgcataag gcatttttct ctttatcttt
     3181 gtacggtact tcatggaaca gagtttttga ccttgcgaat cgtgatgtct gttggggagg
     3241 ggcaatttgc tcactgaagc gtgagactcg attaagcgca cgaaacacag aaatcaaaaa
     3301 acccggtcac ttttttacaa ggtaaccgtg taaaaataat ttttattttt taactgtttt
     3361 gagactcata gagatgtctc aaaactaaaa tttggctcct ctgactggac tcgaaccagt
     3421 gacatacgga ttaacagtcc gccgttctac cgactgaact acagaggaat cgtgagaacg
     3481 aggcgaatat tagcgatgcc cacccacaat gtcaaagcct gttttttaaa tttgaaatcg
     3541 tttgctgaaa taatctgcat tttgtcgttt attccgacac aactggcttt ttttcacact
     3601 tttgcggttc ggatcgaggg tatttccaca gccaacgtcc agtaaccatt cgccagtaaa
     3661 acagcacggc ccgcacagcc cagtcggcaa acattcccat ccagacacca accacacccc
     3721 agccaagcat gatgcccagc acataaccga cgacaacccg gcaaccccac atgctcaaca
     3781 tagaaaccca catggcgtaa cgggcatcgc gagcaccttt aaatccagcg ggtagcaccc
     3841 atgaggcgga ccaaataggc ataaataaag catttagcca aatcagaatc acaacgacat
     3901 gtttaacctg tggatcctgg gtgtaaaacg atgccataac cccggcaaag ggagccgtta
     3961 gccaggcgat ggccgttaat ccaagagtgg aaagccagaa cacatgccgc aactgaatct
     4021 ctgcttgcgc tatctgccct acccccaacc ttcggcctgt aatgatcgta gaagcagagc
     4081 cgagcgcact tccgggtaag ttgataagag ccgcaattga aaacgcgata aaatttccgg
     4141 caataacact ggtccccatc ccggcaacga acatttgggt taataaccga ccactggtaa
     4201 ataacactga ttcgacactc gcgggaatac caatccccat gacttcccag ataatgctaa
     4261 aattcagcgg tttaaaatag ctctttaacg aaatccttag cgcaggatta aaaccaatcg
     4321 ccagcaccca caaaattgca actgcgccaa tataacgaga aatggttaaa cccagccctg
     4381 ccccgacaaa tcccagtccc ggccaggaga aaaggccgta aatcaatatg ccgctaataa
     4441 taatattaag aatattcagg ctaccgttaa tcaatagcgg tattttcgta ttccctgcac
     4501 cacgaagtgc cccgctacca ataagagtga tggcagctgc tggataactg agtaccgtca
     4561 gctccagata agtcaacgcc agtgctttaa cttctgtcgt ggcatcaccc gcgacgaaat
     4621 caataatttg ttcgccaaaa tgatgaataa gcgttgccaa cagtacggca aacaacgtca
     4681 tgatcaccaa tgactgccgc gtcgccaccc tcgctcgtcg tcgatcccgc ttaccgagac
     4741 taaatgccac aacgacagta gtaccaagat cgatagcagc aaaaaaagcc ataatgacca
     4801 tattgaagct gtccgccaat cccacgccgg ccatcgcatc ttttcccagc cagctgacca
     4861 gaaaagtgct cagaaccccc atcaacagga cacaggcatt ctccatgaag ataggaacag
     4921 caagcggggt tatctcgcgc cagaacaaca ctttgtagct cttgcgttta gcgtgccagc
     4981 gagtgccgtg aacaacctgg cgtaaagcag aggagatatt caaagccgac cttaattgca
     5041 gaaagtgaaa ccacatttca aataatgagg gagaatcagc aaagctgcaa agattttcgc
     5101 caacaaattg tctgcaaatg caacaaactg ttgatagaaa cggcaaacag ttggggaatt
     5161 taaaaatcgg gtttgacaaa agatttttcg ccgttaagat gtgcctcaac agcgattcct
     5221 ctgtagttca gtcggtagaa cggcggactg ttaatccgta tgtcactggt tcgagtccag
     5281 tcagaggagc caaatttctg aaaagcccgc ttttatagcg ggctttttgc tatatctgat
     5341 aatcaatttc ctcttcactg ttttccatca cctgccgctt gatatcctca actgacagtc
     5401 ctgcattaca aagttccaga aagcgccaga catagttacg ctgaagttgt cctcgcttca
     5461 gtcccaacca gacagtatta gcatcgaaaa gatgccgcgt atccagacgg attaaattct
     5521 cttcctcttg ttcgccgctg gattgttcgg caactaatcc gatcccaagc ccaagagcaa
     5581 cataggtttt aatgacatca gaatcctgcg cacttaatac aatatctgcc agcaaacctt
     5641 tgcgggcaaa tgcgtcatca atacgtgagc gccccgtaat cccctgtcgg taagtgatta
     5701 acggccactt cgctattgat tccagcgtca atggtgtaat ttgcgttaag ggatgatcga
     5761 gtggaacaag caaactatgg tgccaacgaa accacgggaa ggcgacgagc tgcgggtcat
     5821 tactcaaacg ctcgctggcg ataccaatat cagcttcgcc attttgcaac aacgtcgcaa
     5881 tttcctgtgg cgtcccctgg attagctcga gccgaacctc cgggaaaagt tcgcgaaaag
     5941 ctttaatgac ctctggcaag ctataacgtg cctgagtatg cgtcgttgca atagtgagaa
     6001 cgccagacgt atcgttggta aacaggtctg caagccgacg aacattactg gcttcattca
     6061 gaatacgttc tgcaatgacc agtaatgctt tgcccggttc agtcatgccc agcagtcgct
     6121 tacctcgtcg aacaaatatt tcgatgccaa gttcatcctc cagttcccga atatgacggc
     6181 tgacgccgga ctgtgaggta aaaagcatat tcgcaacctc tgtcaggttg taatcctgac
     6241 gtgcagcctc gcggattatc tttagttgtt ggaaattcac ggtaaactcc gggcagttca
     6301 gatttcccgt tattgttaaa gtctaatgcc cggcataaca aataataaaa acccgcatct
     6361 tattccatcc cgatataacg cttagctcac caat
//
LOCUS       DAKISA010000169         6378 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.172, whole genome shotgun sequence.
ACCESSION   DAKISA010000169 DAKISA010000000
VERSION     DAKISA010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6378
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.172"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..171)
                     /locus_tag="OVW45_004066"
     CDS             complement(<1..171)
                     /locus_tag="OVW45_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528387.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGR"
     gene            complement(173..328)
                     /locus_tag="OVW45_004067"
     CDS             complement(173..328)
                     /locus_tag="OVW45_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCV5528388.1"
                     /translation="MNTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(531..938)
                     /locus_tag="OVW45_004068"
     CDS             complement(531..938)
                     /locus_tag="OVW45_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCV5528389.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1015..1242
                     /locus_tag="OVW45_004069"
     CDS             1015..1242
                     /locus_tag="OVW45_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCV5528390.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPWKVIHENQA"
     gene            1226..1647
                     /locus_tag="OVW45_004070"
                     /pseudo
     CDS             1226..1647
                     /locus_tag="OVW45_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1725..2524
                     /locus_tag="OVW45_004071"
                     /pseudo
     CDS             1725..2524
                     /locus_tag="OVW45_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2531..3277
                     /locus_tag="OVW45_004072"
     CDS             2531..3277
                     /locus_tag="OVW45_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCV5528391.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRSE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELMTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEVMKTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            3292..3714
                     /locus_tag="OVW45_004073"
     CDS             3292..3714
                     /locus_tag="OVW45_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCV5528392.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            3772..4128
                     /locus_tag="OVW45_004074"
     CDS             3772..4128
                     /locus_tag="OVW45_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528393.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            4297..4962
                     /locus_tag="OVW45_004075"
     CDS             4297..4962
                     /locus_tag="OVW45_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCV5528394.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            5190..5345
                     /gene="hokD"
                     /locus_tag="OVW45_004076"
     CDS             5190..5345
                     /gene="hokD"
                     /locus_tag="OVW45_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCV5528395.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            5595..5882
                     /locus_tag="OVW45_004077"
     CDS             5595..5882
                     /locus_tag="OVW45_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528396.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKEIGFKAGFLLLLKNKLIYNLEQD"
     gene            5921..>6378
                     /locus_tag="OVW45_004078"
     CDS             5921..>6378
                     /locus_tag="OVW45_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCV5528397.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVCN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTSDTALLKSFEAFREWVAIQAGFYTEHFYPDGSRGCRAKSIA"
BASE COUNT         1672 a         1393 c         1731 g         1582 t
ORIGIN      
        1 acggccttct gctttgtaga aggcttcttc gctatcataa tcgccagatt ccagcgcttc
       61 tttagccagc cagatgcgtg ccccagtgcc tgcatttggc tccagttgct ggaggcgttt
      121 tgcatcttct aggagtagag cgataacgtg ttttaattct gtctcgttca ttttactcac
      181 ctgaatgtct tcccaaccaa cgacgtgcgc cagcttcggt tttaaacgtt ttgcttttgg
      241 tatacgtcat ggcggtgaat gtgccgtcct ggttggggaa cacgccgtac accagagatt
      301 cgttgttgcc aagatcgata gtattcatgt tgaccccatt tccccttaac gccggggtag
      361 cggaactgtt tgctgagaac accgtgcggt gtcttgatga atgaaattta gaataaccta
      421 aggtgggcgg tcaagatttt tgcgtagaaa aacctaagtt ttttgatgta aaaaacacaa
      481 gtgtttgaaa gtttgtgctt tttattacag ggtgtggaga aaaaagggga ttatttgttt
      541 gcgcttcttt tgcgagcttt gagtagttct tcaaaaagtt tgttgaaatt ttcaactcga
      601 gcacgcatct ctgacaacag ggccttttgc tctgactcag gcagtgcgtc gaacagttga
      661 agtaactctt tttgatcttc tgtcagaatg gctggctgat tatccgggat cggttcgcct
      721 ggttgtttat cttcatcccc aaaaagaagc caagtcggtg agcactgaag cgcttggctc
      781 agtgcgaata atcttttccc cgccggctgt gtttcatctc tttcccattg agaaattgtt
      841 acgtgagcca ctttgaccag cttacctaat gcggcctgag acagttttaa ttttttacgc
      901 ctatgtaaga ggcgagcacc gaaggtttcg tttttcatat tagggaattc taatttttct
      961 tgacttaggt ttctctacga tctagtttcc ttaggaaaat ctaaggagct cgatatgttg
     1021 aaaattgatg ctatagcgtt ttttggcagc aaaacaaagc ttgccaatgc cgcaggagtt
     1081 aggctggcaa gcattgctgc atggggggaa ctggttcctg aaggtcgcgc gatgcgcctg
     1141 caagaggcat ctggcgggga acttcagtac gaccccaaag tttatgacga atatcgtaag
     1201 gcaaaacgac cttggaaggt gattcatgaa aatcaggcat gagcacatcg aatcagtgct
     1261 gttgccctgg cagccgaaaa agggcaggcg tgggtcgcta acgcaattac tgaagaatat
     1321 ctgcggcagg ggggcggcga attgtctctg gtaccaggca aggactggaa caatcagcag
     1381 aatatctatc accgttggtt gaaaggtgaa acgaaagcgc aaagggaaaa aattcagaaa
     1441 ctgatccctg cggttctggc aattcttccg cgtgagctgc gtcaccgact ctgtatcttc
     1501 gataccctgg aacgccgtgc attactggcg gcgcaggaag cgttgagtac ggcaattgat
     1561 gcgcatgatg atgctgtcca ggccgtttac cggaaagcac atttcagcgg tggtgggtcg
     1621 cccggcgatt ctgtcgtagt gcattgattg aaattaatcg tgccggattg ttttgttcgg
     1681 tatcagttaa atgtaacgct gcgagcgtta caaggtgaaa acaaatggct tcaaactgga
     1741 taaagctcga ggttattacg ccggataagc cggaaatatt caggcttgct gagattctga
     1801 atattgatcc agatgccgca ttagggaagg ttattcgctt ctgggcatgg gcggatcaac
     1861 aaatgataga cggtaatgca gattgtaacg ctcgcggcgt tacaaaaagt gcaatagatc
     1921 gcatcacttt tatgtctggt tttgctgatg cgttaattca ggttggatgg ctggtcgaaa
     1981 atgacggtgg gctttctcta cctaactttg aacgtcataa cggaaaaagc tctaaaaaaa
     2041 aacgggcggt tacaaacgag cgagtaacaa aaatacgcga actgaaacga aaaggtaacg
     2101 ctgccagcgt tacacaaacg gatcaaaaag cgttaccaga ggaagaggaa gaggaagagg
     2161 aagaggaaga ggaagatcta aatactgatc tccccctcgc caaaaacgag cgtctaaaaa
     2221 attcgagccg gaggctattg agctgcccga ttggttgccg gaaacactct ggcatgagtg
     2281 ggtccggttc agacaggcat tgcgaaaacc gattcgaacg gagcagggcg ctaacggggc
     2341 gatacgggaa ctggaaaaat tccgtcagca gggttttaca cctgagcagg tgattcgaca
     2401 cagcatcgcc aatgaatacc agggcctgtt cgcgccgaaa ggtgttcggc ctgagacgtt
     2461 gctccgacag gttaacaccg tctcgttgcc ggacagtgcg atcccgccag gcttcagggg
     2521 gtaacggacc atgaaaaata ttgcgacagg cggcgttctg gaacgcatcc gccgactgac
     2581 cccgccacat gtaaccgccc cattcagaac ggtagcggag tggcgcgagt ggcaacttgc
     2641 tgaaggccag aaacgtagcg aggagatcaa ccgcctgaat cgccagttgc gggtggaaaa
     2701 aattctgaat cgctcaggca tccagccgtt gcaccgtaaa tgctcgtttg cgaattacca
     2761 ggtgcagaac gacggccagc gatacgcgtt aagccaggcg aaatccatcg ccgatgaact
     2821 gatgaccggg tgtacaaatt ttgcgttcag cggaaaacct ggtaccggga agaatcactt
     2881 agcggcagct atcgggaatc gcctgctgaa agacggtcag acagtgattg tggttaccgt
     2941 ggctgatgtt atgagcgccc tgcacgccag ctatgacgac gggcagtcag gcgaaaaatt
     3001 tttgcgggag ctgtgcgaag tggatctgct ggttcttgat gaaattggca ttcagcgcga
     3061 gacgaaaaac gagcaggtgg tgctgcacca gattgttgat cgccggacag cgtcgatgcg
     3121 cagcgtgggg atgctgacaa acctgaacta tgaggtgatg aaaacattgc tcggcgagcg
     3181 ggtgatggat cgcatggtca tgaacggcgg gcgctgggtg aattttaact gggagagctg
     3241 gcgtccgaat gttagccatt cgagggttgt taagtagttt caggaggatt tatggcgaaa
     3301 ccttttactc ccgaacagcg ggaagaactg aagacgcgaa ttgtggaact cgtgcatcag
     3361 gacggtcggg tcacgattcg gcagttgtca gatgaaacag gtatcagtcg tgcgtctgtc
     3421 ggtcgcttat gcatagaact ggtcgcaagt ggtgatgtat ataattctgg ctacggctta
     3481 ttcccgtctg aacaggctcg caaggactgg caaagcgccc gcaaaaaact ctcgagagta
     3541 aaggtgagga aaccggttgt tgttgatccg gaccttatct ggtcattacc tgacggagaa
     3601 atacgccgct acgacaggcg cctgaatata atctgtcgcg agtgccggaa gagcgaagct
     3661 atgcagcgtg tactggcttt ctatcagggt aattttcagg aggcgatact gtgaatgaaa
     3721 ttagctatca ggcttcaatt accgctggca ttcgcatcaa aggagaggag catggaaata
     3781 aaaccagaag atgagttaag caatatcgtt ttatttccgg taaaagagga tgaccctcgt
     3841 aatcaggtta attttcttta tgagccatcg gaaagaccat attgccatca cgcctctgta
     3901 cgggttgacg aaaaagagcg tcaggtccgc tgtaaaatct gcggtgcggt tgtggagccg
     3961 tttgactgga tgctctctgt ggcaaaaaga gaaaccagac tggcagatga tgtaaggcta
     4021 ttgcgccagg aggaacagga aaggcggaga aatatagaaa agctgataca gattgagcgt
     4081 aacgcgaaag cgcggatacg cagggcgaca aaatccagaa ctgaataatt aaatttagca
     4141 ctgttaaaaa tttaatcctt aaccggaggg atttctgcac cctcaaatca tcaggagacc
     4201 gcccgaaagg gcggggagca gtcacacatc tgtttccgat agccccgttc taatgctaca
     4261 ctctttgata tttttatgac cccaataaac atatttatga cagttgctga tttcaaacgg
     4321 cccaaattgg agctcccaaa cggggcaaac aaactactac tgcactcttg ctgtgctcca
     4381 tgttccggtg aggtgatgga ggcgcttcag gcctcgggaa tcgactacac catctttttc
     4441 tacaacccga acattcatcc tcagaaagag tatttaattc gtaaggatga gaatattcgc
     4501 tttgctgaac aacacggcgt gccgtttatt gatgctgatt acgacacaga caactggttt
     4561 gaacgagcca aaggaatgga atgggagccc gaacgaggga tccgttgcac catgtgtttt
     4621 gacatgcgtt ttgagcggac agcgctgtac gccgctgaaa atggtttcag tgtgatcagc
     4681 agttcactgg gcatttcacg ctggaaaaat atgcagcagg ttaacgactg tgggcggcga
     4741 gccgtcgcgc attatccggg catggtgtac tgggattata actggcgcaa gcagggcggc
     4801 tcgtcccgta tgattgaaat cagcaagcgc gaaaaattct atcagcagga atattgtggc
     4861 tgtgtgtatt ctctgcgcga taccaatcta caccgcaaat ctcagggacg ccctcttatc
     4921 aaaattggtc aactccacta cggtaaagaa gagaaggagt gattttatgg ggcacctttc
     4981 tgattgattt catattggcg aggtaagtag aatgactgcg ggtgcttgag gctatctgct
     5041 tcaggcatga acaccaaaag gcagatagag aaaagcccca gttaacatta cgcgtccggc
     5101 aagacgctta acattaatct gaggccaatt tcatgctttg cacatgtagg ttagcctctt
     5161 acatgccgaa aggcaaggag aagcaggcta tgaagcagca aaaggcgatg ttaatcgccc
     5221 tgatcgtcat ctgtttaacc gtcatagtga cggcactggt aacgaggaaa gacctctgtg
     5281 aggtacgaat ccgaaccggc cagacggagg tcgctgtctt cgtagactac gaatccagga
     5341 agtaagagtg accgggcggg gagctgatcc catccccgcc cacctctgat gtgtcaggca
     5401 tcctcaacgc acccgcactt aacccgccca tcgctgtgat ctctcagcgt ttcggcgggt
     5461 tttttgttgt ttatttccgg tgaatttgat tcgcgcacct tcgcagatag aatcgactca
     5521 cttaagtagc gcgcagggag aagagggatg gaccccgaac aggggagagc tatttatctg
     5581 gaaggattct gaagatgaaa atcgaagaat tgcgtgaaat ttttagtgaa aatggcctct
     5641 atgctgtgcg cgttgagaat ggagcgattg tcagccattg ccgcattaga tgtttgcaat
     5701 ctcaacaaag gaagagcggt gctgtgttat tttatttttg taatggactt ctgacggacg
     5761 gttttatttt gcgtgaggac gaatttgtca catcattacg ggttttgaaa gagattggtt
     5821 ttaaggctgg ttttctgctt ttgctgaaga ataaactcat ctacaatctt gagcaggatt
     5881 gaactcctgc tgtgtaacac cgtgccaccg gagaaagccg atggcacata tacaactggt
     5941 caaacaaacc tcttccggat tacttctccc ggcgacgccg gagagttgcg attttctgca
     6001 tcaaatcaaa ataggtgagt ggatacacgc agactttaag cgtgtgtgta actacgcatt
     6061 ccacaagcgt tttttcaaac tcctgcaact gggtttcgat tactggactc cggtcggtgg
     6121 ggcgatcacg cctcgcgaac gaaaactggt gtccggtttc gttgattacc tgtgtgaatc
     6181 agtaggccgg gaacatacgc cagctctgag cgaagccgca gagcaatatc tgaatacagt
     6241 tgcgacacgc agaaccagtg atacggcatt gctaaagtcg tttgaggctt tccgcgagtg
     6301 ggtagccatt caggccggat tttacaccga gcatttttat ccggacggta gccgtgggtg
     6361 tcgggcgaaa tctatcgc
//
LOCUS       DAKISA010000170         6353 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.173, whole genome shotgun sequence.
ACCESSION   DAKISA010000170 DAKISA010000000
VERSION     DAKISA010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6353
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.173"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..784)
                     /locus_tag="OVW45_004079"
     CDS             complement(<1..784)
                     /locus_tag="OVW45_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528398.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTK"
     gene            957..1835
                     /gene="yihU"
                     /locus_tag="OVW45_004080"
     CDS             957..1835
                     /gene="yihU"
                     /locus_tag="OVW45_004080"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCV5528399.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            1860..2747
                     /locus_tag="OVW45_004081"
     CDS             1860..2747
                     /locus_tag="OVW45_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCV5528400.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESTIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            2780..3790
                     /locus_tag="OVW45_004082"
     CDS             2780..3790
                     /locus_tag="OVW45_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCV5528401.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKMLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            3860..5293
                     /locus_tag="OVW45_004083"
     CDS             3860..5293
                     /locus_tag="OVW45_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCV5528402.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            5392..>6353
                     /locus_tag="OVW45_004084"
     CDS             5392..>6353
                     /locus_tag="OVW45_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCV5528403.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAG"
BASE COUNT         1459 a         1459 c         1697 g         1738 t
ORIGIN      
        1 cttttgttgg cattttttct gccaacgcta ccgcgagtgc gccgtgaaaa acatcgcctg
       61 ctccagtggt atcaaccaca ttgacagaaa atgcctcctg ttgacacaaa tgaccatctt
      121 caatccacaa cgatccttca gaacccagcg taacatacac tttacctgct gtttgtgtcg
      181 ttgcctggaa caaaccttct tcaggtgact gcaggccagt catgcgtttc agccctggtg
      241 ttgagaacac ggcgtgatcg gcaagcgcga caagtggaga aatatcctgc ggtgtcatat
      301 cggcgtctaa aagcgttgtc acacccgcaa ggcgcgctag cgagaatgct ttttcagtac
      361 cgctatgcca gcgaacatcg gccagaatca gatcataacg agagaaatcg atcgcttcta
      421 accattcggc atcagtaccg agatcaggac ttggataatt aacgataatc cgttcgccat
      481 gctgatcaac gaggatcgct gattgcgacg aacgggcgtt aggatatcgg cggcaaaatg
      541 cggtattgac gccccaaccc tcaagttctg caagtaaggt atttccacaa ctgtcatcgc
      601 cgacgcgacc gatgaaatca acctccacgc caagctttgc gattgcaaca gcggctgttg
      661 ctgccgggcc gccaccaatc tcaagataat gatttgcctg atatttcccg ccgccctccg
      721 gcagagtggg caaagaataa attctgtcct gaactgtgat acccacgcat gcaattctgg
      781 tcatatttgt cttgtcctgt ttaattttgt cgtctttggt caatttaaat gacgcaaatg
      841 atcaaattag tgatgcgtgt cgtgtttttg aacataaatt acaaatatgc tcaaaaacag
      901 acgaaaacga attttcaatt gtcattattt tgtcaatgtc agacaggaga gtattcatgg
      961 caaccattgc atttctcggg ctggggcaga tgggtagccc aatggctaac aatctattgc
     1021 aaaaagggca ttcattgcag gtatttgatg tcaatgcaca ggccgtcgat gcgctagtta
     1081 cgcagggggc cactgcggca caaaccccag cggaagcagc agcaggcgct gaatttatca
     1141 tcactatgct tcccaatggt gacattgttc gacaggttct gttgggggaa aaaggcgtct
     1201 gtgaggctgt ttcatctgac gcgttattga ttgatatgtc gactattcat cccttacaga
     1261 ccgatgcatt aatacgggag ttacaagaaa aaggcatcag tatgatggac gctcccgtgg
     1321 gcagaacgtc cgtgaatgcg attgatggca cattgctgat tttagcgggt ggcactgatg
     1381 agcagattgc acgtgcacgc ccgattctca tgtgcatggg taatgagctg gtcgaggctg
     1441 gcggcccagg aatggggatc cgcgtgaagc taattaacaa ctatatgagc attgcgctta
     1501 atgcgctgtc ctccgaggcc gctgtgttat gtgagtcact gggattaaat cttgatgtcg
     1561 caattaaggt gatgagtggt acggcagcag ggaaaggaca ttttacgacc acctggcctg
     1621 gcaaagtgct gaaaggcgat ctttcacccg ccttcatggt ggatctggcg ttgaaagatc
     1681 tgcgtattgc agttgacgtc gctcgggaga cgggggcacc gctcaatatg gggattgcag
     1741 cagaaagtta ttacgctgca gcaagtcaag acggcaaagg gcggcaggac tggagtgccc
     1801 tgctaaatca ggttcgccag caggctggtc tttaatttta tctattaaca ggagtttcta
     1861 tgtcatccca cttcacccca tttacgctga aagatatctc ccgcccaggt ggtggatttg
     1921 ccatgttagc tgtcgatcag cgtgaggcca tgcgcttgat gtttgcggca gcagggcagc
     1981 caaaacccat cgctgacagt gtactcaccg attttaaagt tgccgcgacc cgaatccttt
     2041 ctccttatgc ttctgccgtt ctggcggata aacaattttg tcttgagcaa atagtcgagc
     2101 agggcgctgt tgctaattct tgcggcctga ttgtcgctgc cgatcttttt atcccaggta
     2161 atggcatccc ggttgatagc gttgaaatag atatgtccgt cgacccgcat aaagcccgtg
     2221 aaatgggggc taaggcaatg aaattacttg tactctggcg tgaagatgaa cccgcagagg
     2281 aacgtctggc aatggttgat aaatttgttc gtcgctgccg tagtgccggg ctggtgagca
     2341 ttattgaacc tgtggtgcgt ccacctcgtc gcggttggga ctttgatcgt gaaagcacca
     2401 ttgttgccgc cgccgcagag cttggtggaa cagaggctga tctttacaaa gccgagatgc
     2461 cattaggggg caaaggcgat gaaaaaacct tacttgccgc ctgtcagcaa cttaacgatc
     2521 agatgaagat gccgtgggta attctttctt ctggcgttga tgccgatatt tttggccgtg
     2581 cagtaagtat tgccatgaaa ggtggtgcgt cgggcttcct ggctggacga gcggtttggg
     2641 cgtcggttgt tggtgcacaa gatccacaaa ctatgctgcg cgatgtctct gttcctcgtt
     2701 tacaacggtt ggcagaaatt gtcgatgaag gtatcgcgca acgctaatcc cctatggata
     2761 tcgattctgg agaataacaa tggataaaat tacccaggtt ttattttcag atattgggaa
     2821 agtcaccact caatatgttg aagtaccaca ccaggaactt aaaccgcacg aagtgcggat
     2881 tgcgcctgtg ttctatggga tatgcggttc ggatctgcat gttctgaaag gcggtcatcc
     2941 gtttgccaaa ccacctgtcg tccccggtca tgaaattgca gcgcgcgtta cggaagttgg
     3001 cagcgacgtt aaaaatgtac agccgggcga tcatgttgtg gtcgatccca tcatggcttg
     3061 catggaatgc cgagcctgca aagcaggacg ttttaatctt tgtgaaccac cgcaggttgc
     3121 tggttttcgc gcaccgggct ttgctcgctc acaacacatt gttcctgcgc gtaattgcca
     3181 tgtcgcacca gcctctttac cgctaaaaat gttggccttt gccgaaccgg cggcttgtgc
     3241 ccgtcactgc gttaaccgaa tgccgaaagc ttctctggaa agcgtactgg taattggtgc
     3301 cggaacgata ggcttatcca tcgtgcaggc actgcgcatt atgggggcag gtaagattac
     3361 cgtgattgaa cctgacgctg ccaaacgcgc gctggcgtta aagctgggcg cagcagaagt
     3421 ttgggcacca ggtgagctgg ccgcagatgt gcgatttacg ggggcgattg atgtcgttgc
     3481 agcgcaggcc acgcttaacg atgcatgtac ccgtgtatat gccggaggca ccgtcgtgtg
     3541 catgggcgta ccaagtgggc cgcgtgaaat accattaccg atgatgcaac gtttcgagcg
     3601 tgacttgctc aactctggca tgtacatccc tgaagatttc gatgctgtta tcgaatggct
     3661 ggcggatggg cggtttgata ccagtgaact ggttaccgat ttatttgcca ttgaggatgc
     3721 agcggcggca tttgaacgcg cgcagcaaaa tgactccata aaggtcatgc tgcaatttgc
     3781 gccggaatga ttttctaaat ctctgtctgg cgctgcgtca cgtcagcgcc accctacaac
     3841 aattaaaaag gacactgcga tgagtcaaat caccagtcct gcaacttatt caattagtcg
     3901 gccacaggac gtaattgata ttgttaataa gaactctgca atcaacacca gtatcggcgt
     3961 tatttttatt gctttgggcg gcatattgat tgatgcctac caggcggcga tggtggggtt
     4021 tggtaataaa tacattgccg ctcaattcgg catttcgccg ggtcttgctg caaccgtcaa
     4081 tgcatcagta ttaatcgccg cgttaattgg cggtttatta gcgaaccgag taataaaccg
     4141 ctttgggcaa aagcgggcat ttattattgg catggggctg tgcaccatcg gtgctgcagc
     4201 ggtagctatt gctcccagta tctggtgggt gctggtgtgc cgcgtcatca tgggctttgg
     4261 tttaggcatc gacttccctt tggcaaccaa tgccgtggca gagcttcgtg gttcaacgtc
     4321 gaagaaaacc ggaacgtcgg tcaacctctg gcaaatggcc tggtatgttt cgacaaccgt
     4381 tgtttatttg gtacttttgc cgctgcttct gtcgggtatc gctgaagaac aattgtggcg
     4441 ttacggaata ttcatcggag ctatttttgc agtcatcttc atgattttgc gttacttctt
     4501 tattggtgaa tccgcaatgt gggccgcacg cgtcgggcgt taccaggaag cgtgcgacat
     4561 tctgggaaaa cgttatggtg ttcaggctcg cgttgcggca tcgggtacaa cagaagcgaa
     4621 attctcggaa aaagcagaga ataaatacag tggtggatat ggcatcttat ttaatgatcg
     4681 ttaccgcaaa cgcaccattc ttggctgtgt cgtggcaacc atgcaggcgt ggcaatataa
     4741 cgccgtaggt gtttatcttc ctcttacgtt ggcgggaata ataagtggcg ggcttactgg
     4801 cgcgttgacc ggttctgccg tcgtgaatgc cctttgtggg gtgacaggcg ggatgatcgg
     4861 ctcgtttatt ctccaacgac tgggtactcg ccgacagtcg atgtatggat ttgctgttgt
     4921 gaccttagca ttgctgtcgt taggcgcact ggcaacgact aatccctggc tgtctttagg
     4981 gttattggga tcaattattt tcttccattc agcgggtcct ggtgggctgg gcatgaccat
     5041 tgccacactc tcttatcctc ccgctattcg ccctactggg gtcggatttg cccgcgctat
     5101 tatgcgcaca ggggcaattg caggactcat tttctggccg atgctgtggg gtgcgttgaa
     5161 aactgaagcg ttttactggt tggcaatcgt gccattcctg gggttcctga cctgcgtatt
     5221 gattaattgg gaaccactgg gtgcaaatgt tgatgctgag gatgcagagg ttctggctga
     5281 attgaagaaa taaattaatg cgcatcggct taaaccgatg cgcattaatt caaactgccc
     5341 acgtttagtg gttcgtatcc cagatttctc gcctaccagg agaggaaaat tatgtctgaa
     5401 aagttacccg caccgcgtga agggctttcc ggtaaagcca tgagacgtgt cgttatgggc
     5461 agtttcgcag gcgcgttaat ggaatggtat gacttcttta tttttggcac ggcagcagga
     5521 ctggtatttg caccgctgtt ttatccagac agcgatcctt ttataggtct tatagccgcc
     5581 tttgcgacct ttggtgtcgg ttttctgact cgtcctttag gtggcatcgt ctttggtcat
     5641 tttggcgata aaatcgggcg aaaaataaca cttatctgga cgctggctat tgtcggttgc
     5701 tccacatttt tgattggttt tattccgacg tatcaggaaa ttggcatttg ggcaccgctt
     5761 gtattaatgg tgctgcgttt gattcagggc tttggtcttg gtggcgagta cggcggagcg
     5821 gcattgatga ccatagaatc agcgcccgaa tcccgacgcg gatttttagg ttcactcccg
     5881 caaacggctg cgtctgtggg tattatgctg gcaaccggta tttttgctct ctgtaaccat
     5941 tttcttactt cggagcagtt tctctcctgg ggatggcgca ttccgttttg gctttctgcg
     6001 gtgatgttga tcgttgggct gtttatccgc ctgcataccg aagaaacact ggattttcaa
     6061 aagcaaaaaa cgacgaataa taaagaaaag tccgttcctc cgttgattga attattcaaa
     6121 aaacatccac gaaatatttt attggcactg ggtgcgaggc tggcggaaag tgtctcctct
     6181 aatattatta acgcctttgg tattgtctat atttccagcc aattagcatt gtcgcgagat
     6241 attcccctga cgggtatgtt gattgcctcg gcgatcggta ttttcagttg tccattggtt
     6301 ggatggttat cagatcgtat aggtcaaaaa agtctgtatt tgtcgggagc agg
//
LOCUS       DAKISA010000171         6222 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.174, whole genome shotgun sequence.
ACCESSION   DAKISA010000171 DAKISA010000000
VERSION     DAKISA010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6222
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.174"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(358..1401)
                     /locus_tag="OVW45_004085"
     CDS             complement(358..1401)
                     /locus_tag="OVW45_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCV5528404.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNASLKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            complement(1812..2996)
                     /locus_tag="OVW45_004086"
     CDS             complement(1812..2996)
                     /locus_tag="OVW45_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCV5528405.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQMSLKNTFEA
                     IAREWHQSKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            complement(3296..3390)
                     /locus_tag="OVW45_004087"
     tRNA            complement(3296..3390)
                     /locus_tag="OVW45_004087"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3354..3356),aa:Sec,seq:tca)
     gene            3683..5065
                     /locus_tag="OVW45_004088"
     CDS             3683..5065
                     /locus_tag="OVW45_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HCV5528406.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            5075..>6222
                     /gene="yicI"
                     /locus_tag="OVW45_004089"
     CDS             5075..>6222
                     /gene="yicI"
                     /locus_tag="OVW45_004089"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCV5528407.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPG"
BASE COUNT         1610 a         1388 c         1468 g         1756 t
ORIGIN      
        1 ccacgggaat atctgcggca gcgggcttgt aatgggttaa gtgataacag atgtctggaa
       61 atataggggc aaatccacgg ggataccagt tcaaccgaaa acgccagagg aggggattac
      121 ccgctggcag ggtaaatctg tggtttttga tgccagctat aacgtgtttg acgataaagt
      181 attacgaaag aactttaatg agttcatgga gtaattcgtt aaagtaattt acaacgctat
      241 ccttttgcaa aggaaacttt cagaaaataa ccccagggta atcacctggg gaaggaacac
      301 agtgttatta ttggataata aattattact tgttttaaag ttacactaat gcaatatcta
      361 ctgatactgg atgcttgaat gtttcatccc tgctgcggaa aagggcaata tccagggcca
      421 gatatattca ccgttcatat ttctgcattt ctgtacaatt tcctcagaaa gtgtcttgtc
      481 aattcttttc cacaggtgct cctgctgtct taaaatactt tcgcatattt cgaaatcatc
      541 ttcctgaatc agcatttcgg cgcatttaat gatttctgat cgttcatgca gcacggagga
      601 atggttatag agccagttta caaacagcaa tgcttccgga tattgtcccc ataatggtga
      661 gtatgttgca tagtaatctt ctgccgtatc cagagagccg gagaagtcat gccacacagc
      721 aagtggaaat ggatgcagat ttccgcacag catttctgcg atgtaagtgt ttgtggcaaa
      781 gccatggcga aaagctgtgg cggccttaac atgttccccg ttcagaaaat gcaggagacc
      841 gagttcatac cagtaaggtg aaaattcatc agcatgctct ttcagtacac tgaaggctcg
      901 ctcatgatcg ccggtgcgta acagctctga acccaacaac cagcgagccc cctgattatc
      961 ttcaggattg tacgccagta ttttatcagt gagcataacc gcatcctgat ggcgctgtaa
     1021 gtgcacattt gcgagaattg ctgcataaag cgcgcgcaga taaggccggt tttcgggatg
     1081 catccagatt atttcaccac agaaactttc cgggatgata cgattacctg ccgccagtcc
     1141 tttaagagag gcgttcagtg ccttacgcgg ggcattctgt tccagaaatg catatgccag
     1201 atgagcgtgt atatcaataa agtccggctc ccgttgagca aggcgtcgga gtttgttgat
     1261 gtaactcgtc tcgctgattt catcagcgtc gtatgcatca agcacttctt caaactcccc
     1321 cagcaatggg ccaaaccagg tatccggaaa tacgaaataa cccttgttat cgtgcacttc
     1381 aaagcataat ctatcgttca tttctgagcc tgtaaaacat gtggtgatat agtctgagag
     1441 cataaaagtt gagattctgt gtatcaagtc ctgtcttggc accgaatgaa tattgcttac
     1501 gaagcccctt catgctttcc ttgagaacaa taattttatc tttatgtgaa tctcattttt
     1561 tataatgatg tcagatgatt gaaatgctaa attatatctg aatcaacttt tcctttgcta
     1621 aaggtatcga aataactgca aggctgtatt ttgtaattgc caatttaaaa atgtcaattt
     1681 gtttttaatt atgtttttgt aataagtttg aagacgcaaa aaaataatat aataacaaaa
     1741 atcctccagc cttgtaggcc agaggataaa atacttaatt gaggaaaata tcagggaaaa
     1801 gtatttctga cttaactttc ccggctggag agtgaatcaa tgtaatcagc gtaccattgc
     1861 atcatttcac ggcggccatc gagatacagg gcatgattat aggtacccct gatggaattt
     1921 ttatccacat gagctaactg catttcaatc caggcggaat taaagccttg ttcatgcaga
     1981 attgtactca tggtgtgtct gaaaccgtgc cctgtgagtc ttcccttata tccaagtaat
     2041 tcaattactt ggttaatgct ttccttacta attggtttgc ggggatcatt tctaccgata
     2101 aagactaggg gatgatgttt tgagattggt tcaagctgct taaatagcat aatgacctgc
     2161 tcagagagcg gcacaatatg aggtcgcttc atcttcatta cttctgccgg gatctcccac
     2221 aattttgtct caaaatcgat atcttcccag cgtgcaaaac gcagttcttg tgttcgtacg
     2281 ccggttaaca taatgatctg agtagctgtc ttagtgatta tacttccggt ataacccgcc
     2341 agatcgttga ggaagtgggg aagttcatta gcagtaagaa agggaaaatg tacttttttc
     2401 ggtgtagcta aagcactggc aagatcgggg gctgggttat agtctgcccg gccagtaaca
     2461 attgcatagc ggaacacctc accacagcgc tggcggacct tgcgcatttt ctccagagct
     2521 ccacgttttt ccattttacg cagtgcttcc agtaactcca ttggtttgat ttcggcaata
     2581 gggcgcttgc caatgtaggg aaaaatgtct ttttcaaagg tatcaatgat ttcatcacga
     2641 tagcgtaatg accaccgatc agcttttgac tggtgccatt cgcgagcaat agcctcgaac
     2701 gtatttttca gactcatttg ctgcgcgatt ttttcttcct tttttgcctc ccctggatcg
     2761 cctccagcag cgaggagttt cctggcttca ctgcgtttct cgcgagcatc ggctagcgtt
     2821 acatcggggt atacgccaat agaaagcatc ttttctttac cagcaaagcg gtacttcatg
     2881 cgccagtatt tagaaccatt agtgttaatc agtagataca taccgccgcc atcagcaagc
     2941 ttatagggtt tgtctttggg ttttgcggtt ttcactttga tatcggttaa tgccatgaca
     3001 tcctccggct tttgatgaat tcatgttcct gagggggatt tctgccagta actaacctga
     3061 tacccccaat acaccccttg tataacaaag atattgttaa aagccgttgt tatgtatcac
     3121 atttttccta ttatgggggt gcttgtattt caataggaaa taacaccccc taaagtaccc
     3181 ccaaaagaaa cttgatatgg ggtgaagtag gttgacctca ggatacaaat aaatactgat
     3241 atacaaggga ttagaagaga tttattgaca ttgggagagt tcagaagagg taaactggcg
     3301 gaagatcaca ggagtcgaac ctgcccggga ccgctggcgg ccccaactgg atttgaagtc
     3361 cagccgcctc accggagacg acgatcttcc gcgcctcgat tgctacatgg aggcggggcg
     3421 cattatagct acttccttga gtttctacat cccccagatc agttttttcc gcctaataca
     3481 cgcagcgtat tatcacgaaa gcattataac tcatataaaa cccatatttt acaatgatat
     3541 atgagcggaa ttaacgctac cacgatcaca taaaacaaga atcataaatt aataaccaga
     3601 tatcggaata ttcgctctca cagggatggt ttacaaaatg cgttatcggt ctcagcaccc
     3661 ctatagcatc aaggaaaagc agatgaagag tgaagtgttg tccgttaaag agaaaattgg
     3721 ttatggcatg ggagacgccg ccagccacat tattttcgat aacgtaatgt tatatatgat
     3781 gttcttttat accgatattt ttggcattcc tgccggattt gtcggaacca tgtttttggt
     3841 cgctcgtgca ctggatgcga tttccgatcc ttgcatgggg ttgttggccg atcgaacgcg
     3901 ctctcgctgg ggtaaatttc gtccgtgggt actgtttggc gcactgccat tcgggatcgt
     3961 ctgtgtactg gcctatagca cgccagatct cagtatgaac ggcaaaatga tctatgcagc
     4021 aattacttac accctactta ccttacttta taccgtcgtc aatatccctt actgcgcatt
     4081 gggtggtgta atcaccaatg acccgactca gcgtatctcg ctgcaatcct ggcgttttgt
     4141 gctggcgacc gcgggaggca tgctttctac tgttctgatg atgccactgg ttaatttaat
     4201 tggcggtgat aataaaccac tcggtttcca gggcggtatc gcggtccttt ccgtggtggc
     4261 attcatgatg ctggcatttt gtttcttcac cactaaagaa cgcgttgaag caccacctac
     4321 aacaacgtct atgcgggaag atttacgtga tatctggcaa aacgaccagt ggcggattgt
     4381 cggtttacta accattttca atatcctggc ggtgtgcgta cgcggtgggg cgatgatgta
     4441 ttacgtcaca tggattttgg gcacgccgga agtgtttgtc gcttttctca ccacttattg
     4501 cgtgggtaac ctgattggtt ccgcactggc aaaacctctg accgactgga aatgtaaagt
     4561 cactatcttc tggtggacga acgccctgct ggcagtgatt agcctcgcga tgttctttgt
     4621 tcccatgcag gccagcatca ctatgtttgt cttcatcttc gtgattggtg tgttgcatca
     4681 actggtgaca cctatccagt gggtaatgat gtccgatacc gtcgactacg gcgagtggtg
     4741 caatggtaaa cgcctgaccg ggatcagttt tgctggcacg ctgtttgtgc tcaaactggg
     4801 gttggccttc ggcggcgctc ttatcggctg gatgctggct tatggcggat atgatgcggc
     4861 agaaaaagcg cagaacagcg ccacgattag catcattatt gcgctattca cgattgttcc
     4921 ggcgatctgt tatttgctga gcgcgattat cgctaaacgc tactactcac tcacgacgca
     4981 caatctgaaa accgttatgg aacagctggc tcagggtaaa cgccgttgcc agcaacaatt
     5041 cacctctcaa gaagtgcaga actaaggaac ggcaatgaaa attagcgatg gaaactggtt
     5101 gattcaacct ggcctcaatt tgattcaccc gcttcaggtg ttcgaggttg aacagcagga
     5161 taatgaaatg gtggtctatg ctgccccccg tgatgtgcgt gaacgtacct ggcagcttga
     5221 tacgccttta tttacgctgc gctttttctc cccacaggaa ggtattgtcg gtgtgcggat
     5281 tgagcatttt cagggggcgc tgaataacgg tcctcattat ccgctcaata ttttgcagga
     5341 cgtgaaggtc acaatcgaaa acacagaacg ttatgctgag tttaaaagtg gcaacttaag
     5401 cgcgcgtgtc agcaaaggtg agttctggtc actggatttt ctgcgcaacg gcgaacgtat
     5461 taccggtagt caggtgaaaa ataatggcta cgtgcaggac acgaataatc aacgcaatta
     5521 tatgtttgag cggcttgatc ttggcgttgg cgaaacagtt tacggtctgg gagagcgctt
     5581 tactgccctg gtgcgcaatg gccagacggt agagacctgg aaccgggacg gcggcacaag
     5641 tactgaacag gcgtataaaa atatcccgtt ctacatgact aaccgtggtt atggggtact
     5701 ggtcaatcat ccccagtgtg tctcttttga agtgggatcg gagaaagtct ccaaagtgca
     5761 gttcagcgtt gagagtgaat atctcgaata ctttgttatc gacggcccga cgccgaaagc
     5821 ggtacttgat cgttataccc gctttactgg tcgtccggcg ctgccgcccg cgtggtcctt
     5881 cggcctgtgg ctaaccactt catttaccac caactacgac gaagcgacgg taaacagctt
     5941 tatcgatggt atggcggaac gcaatctgcc gctgcatgtt ttccactttg actgtttctg
     6001 gatgaaagcc ttccagtggt gcgattttga gtgggacccg ctgactttcc ctgacccgga
     6061 agggatgatc cgccgcctga aagcgaaagg gctgaaaatc tgcgtctgga ttaaccccta
     6121 tatcggtcaa aaatcccccg tctttaaaga gttacaagag aaaggctatt tactcaaacg
     6181 cccggacggt tcgctatggc agtgggataa atggcagcca gg
//
LOCUS       DAKISA010000172         6201 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.175, whole genome shotgun sequence.
ACCESSION   DAKISA010000172 DAKISA010000000
VERSION     DAKISA010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6201
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.175"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            12..754
                     /locus_tag="OVW45_004090"
                     /pseudo
     CDS             12..754
                     /locus_tag="OVW45_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(796..1248)
                     /locus_tag="OVW45_004091"
                     /pseudo
     CDS             complement(796..1248)
                     /locus_tag="OVW45_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1312..1458
                     /locus_tag="OVW45_004092"
                     /pseudo
     CDS             1312..1458
                     /locus_tag="OVW45_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1458..2615)
                     /gene="intS"
                     /locus_tag="OVW45_004093"
     CDS             complement(1458..2615)
                     /gene="intS"
                     /locus_tag="OVW45_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HCV5528408.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIVPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQMPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMLWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE"
     gene            complement(2777..2851)
                     /locus_tag="OVW45_004094"
     tRNA            complement(2777..2851)
                     /locus_tag="OVW45_004094"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2816..2818),aa:Arg,seq:cct)
     gene            complement(2927..3859)
                     /locus_tag="OVW45_004095"
     CDS             complement(2927..3859)
                     /locus_tag="OVW45_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCV5528409.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            4153..4908
                     /gene="mlaA"
                     /locus_tag="OVW45_004096"
     CDS             4153..4908
                     /gene="mlaA"
                     /locus_tag="OVW45_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCV5528410.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(5090..5425)
                     /locus_tag="OVW45_004097"
     CDS             complement(5090..5425)
                     /locus_tag="OVW45_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528411.1"
                     /translation="MAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDD
                     AKFRRDNVKEIISNLFANKVDVDHPENKYKDFKDLEDNVEKRLQNRQAKYQNEINQLS
                     APGVNFDDI"
     gene            complement(5383..5775)
                     /locus_tag="OVW45_004098"
     CDS             complement(5383..5775)
                     /locus_tag="OVW45_004098"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528412.1"
                     /translation="MIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGNSEPTDLF
                     TWYGKDKKGNSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGDSLTQYCSEKARN
                     ASAMLQRGLKAVKLSWQNHLQQVPIIRL"
     gene            complement(5782..6158)
                     /locus_tag="OVW45_004099"
                     /pseudo
     CDS             complement(5782..6158)
                     /locus_tag="OVW45_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937824.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1546 a         1371 c         1374 g         1910 t
ORIGIN      
        1 gggtggatga tatggcaact acatgttcag ttatattgat tttggagtcc tttgatgttt
       61 atttcggaaa agagagtgtg tttctggaga gaggttcatc tgtacttgtc gactctagct
      121 ctagagattt tttcctgaca tatcctgaaa gagtgatagt ggcggatttt ggcgctgagt
      181 ttattagtcg ctatttgaaa gctaataact taagggatat ttctgattgt agggaatatc
      241 catcttattt aaaaataaac tttgctgact tcagtttaat taaaggatta attagttggg
      301 ctaatcactg tgctgaatac atagaaattt ttgatgagtc tattgctttt acatgtctct
      361 ctgcattttc ttctgaaaaa caatttggag tatttctgtt tggatgtttg aaaagcacag
      421 ggctaaagtt aaaacgatta ttcatacgga tttatctgca ccatggcgtc ttaaggatat
      481 atcatcaaga ttatatctca gcgaaagttt actaaagatg aaattgaaag aagagggggt
      541 atcattcagt aagatcatac ttgatgagag gatgcaaatg gctgaatatt tactcagcac
      601 tcgttgttat cctattagta aagtagctaa ggtctgtggt tatgccagtg tctcatactt
      661 tacttatgta tttagacgtt attttggtgt ttctccaagt caatactctc agaggagttc
      721 agaaagtaaa attcttactc accagggaat ctgatcattg ttcttgcccc cttatttcca
      781 gacagggggt gtatcttaag ttaacgttac ccgctgacgt cgatattctc gcggagagcg
      841 ataacccagc gcactgtgcg gatggttttc attgtaatgt tcgatcgcca ctgcaagatt
      901 atgcaatgcc gttcttacat tcggtttcgg catgaacgcg atgtagtctt ccttcatcgt
      961 tttcacgaac ctttctgcca ttccattact ctgaggactg ctgattgccg ttgtgcaggg
     1021 ctccagattc aactctctgg cgaactgccg tgtttcatgc gctctatacg ctgaaccgtt
     1081 atctgtcagc cactggatgg actgttccgg tactttatcg ccaaagcgtt tttctatcgc
     1141 tcctaacatg acatcctgca ctgtcgcttt atcgtaacct cccgtgcttg cggcccagtc
     1201 tatggcttca cgatcgcaac agtccagggc gaacgtaaca cgcagttttg aatcgccacg
     1261 ggtttaacag acacctcaga gtcatttaag atggcttaaa gagaggtgcc catgagcggt
     1321 aagcgttatc ccgaagagtt taaaactgaa gcagtcaaac aggttgttga tcgcggttat
     1381 tctgttgcca gcgttgcaac acgtctcgat atcactaccc acagccttta tgcctggata
     1441 aagaagtacg gtccggatta ctccactttt tcatcaagcc aatccgccca ccactgcatc
     1501 atttctctgc gcttatcgag atactgagca tggttgtaaa taccgcgcac agatccgccg
     1561 ttggcatgtg ccagctgcac ttcaatagcg tcagcaggcc attcgtgctc gttcataatc
     1621 gtgctgaatt catgcctgaa tccgtgaccg ctttccagac cttcatagcc gatttgtttg
     1681 atcacaagca ataccgcgtt ctcgcagatt ggcttcttct tatcgttgcg cccggcaaaa
     1741 acaaactctg agactggttt ggtgattgag cttagcgtag tgagaagttc aaccacctgg
     1801 tctgacatcg ggaccacatg aattttgcgt cccttcatca cactggcgtc gatggtgata
     1861 atcctgtttt caaaatcgac gttcttccat agcatggaac gaagctcttt tgttcttagg
     1921 gctgtgtagc gtaaaacttt ggtcgcaatg agcgatacga tacttcctga aaatgttgcc
     1981 agtgctttgt tgaatgcagg catctggtct gctggaagga acgggaagtt cttcttgcgg
     2041 tatcctttca tggcgtctgc aaggtcaggt gccgggttat atttagccct tccggtgaca
     2101 atagcgtaac gaaaaacctc gccgcatctt ctgcgggctt tgttggctcg ctccattgca
     2161 ccgcgatctt caaatctgcg gattacttcc agcagttgca tcggctcaat atcctgaatc
     2221 tcaagaccgc cgatgatggg taaaatgtcg tcatcaaaca ttttggcaag ttcagttgca
     2281 tagcctactg accagacttg cttcttgtgc tcgtaccatt ccttgtaaat cgcactaaat
     2341 gaattgttgt tagacgaagc ctttttcgcc tttaccggaa cgatgccaac cgagatgtct
     2401 ttcctcgcag tccatgcctt atcccttgct tcctgcaaag tcataagcgg atattttccg
     2461 acggtcagga ttttctcctt accgtcaatc ttgtagcgaa gctgccatac ctttttcccg
     2521 gatacaggaa cataaaggta caggccatta ccatcgagaa ggcggtatgg tttttctttc
     2581 ggctttgctg cttcaatctg cttaacggtg agcatgggta aaaatccggt gggtaaaatt
     2641 attttatcca ctttttaccc gtcatggagt gcggctgtca acgatctgac gcgaaccatg
     2701 acgaaccgtg aatatacgga aggcttgata ttcaggggat tttgcggact ggtacggatg
     2761 ggagcgaact gataaatggt gtcccctgca ggaatcgaac ctgcaattag cccttaggag
     2821 gggctcgtta tatccattta actaagagga caatgcggca tgagtatacc cgctaatgga
     2881 gtgcggggta agtacgctgc cgctcgattg cttaaaccct cgccatttat gccgggtttt
     2941 tatcattttt cttaatgttt tccgcacgtt ctgctttttg gcgtgcttct gctttacgct
     3001 tgttgctcat gtcgttacga atctgtgcat gactcattaa cgcgaagata aaggtgccgc
     3061 cgcagatgtt ccccgctaaa gtaggtagtg cgaagggcca gatgaaatcg ctccagtgca
     3121 gcgtaccgtt aaacaccaga tagaggattt caacagaacc gaccacgata tgggtggtgt
     3181 cacccagggc aataagccag gtcatcaata taatcaccac aatctttgcc gcacctgctg
     3241 caggaaacat ccataccata gtggcgatca gccagccgga aatgatcgcg ttggcaaaca
     3301 tctcgctggg ggtgttcttc atcacatcca tgccgatttt gacaaatgca tcgcgagttt
     3361 cttcattgaa gataggcata tattcaaatg cccacgcagc aataccagtc ccgagaatat
     3421 tacccagcag cacgactccc cataaccgca taagtaagcc aacgttgctc attgtcggtt
     3481 tttgcatgac gggtagtacc gcagtcacgg tgttttcggt aaataattgc tggcgggcca
     3541 ttatgacgat aataaaacca aaggtataac cgagattctc cagtaagaag ctgcccggca
     3601 caccttccag ttcgacatga aatatccctt ttgccagtaa cgaagcgccc atcgacagac
     3661 ccgccgcaat ggctgaccac agtagcgcca ttgcgtcgcg ttccagctct ttttcaccat
     3721 cctggcggat atgctcatga attgccatcg cccgggaggg gagtcggtct tcatctattt
     3781 ctattttttt gccgcgctct ttttcttcgc tctcaacttc aatttcgtcg ctgtgttgat
     3841 caattttgtc gttgtccatg gtctcttctc ttgaattagc acgtatagct aaagcgtagc
     3901 ggcttttttg ctcgcaactg gcgggagtta ctctgaaaat gtagaaaagg ctgcgtttgc
     3961 ctttttctgt ttctatagaa tcaagtagcc tacagggcgg cgattaccag gctatgatca
     4021 aatcagcaaa tcagggcgtc tggacatcag ttgacgtgct gttacaatcg cccacaccta
     4081 aacaggcgga tacggtatcg ttccgtcatg gatggcaaac tgcataagcc ataaaaaaac
     4141 agggagacat ttatgaagct tcgcctgtcg gcgcttgctc tgggaactac gcttctggtg
     4201 gggtgtgcga gttccggtac agatcagcaa gggcgttctg acccgttaga agggttcaac
     4261 cgcaccatgt acaacttcaa cttcaacgta ttagacccgt atattgttcg accggtcgct
     4321 gtcgcctggc gtgattatgt tccgcaaccg gcgcgtaatg gtttgagcaa ctttactggc
     4381 aaccttgaag aacctgcggt gatggttaac tacttcttgc agggcgaccc ttatcagggg
     4441 atggtccact ttacccgctt tttcctgaac accattttgg ggatgggcgg ctttattgat
     4501 gttgcaggga tggcgaaccc gaaactgcaa cggactgaac ctcaccgctt cggtagtacg
     4561 cttggtcatt atggcgtggg ttatgggcct tacgttcagt taccgttcta cggtagcttc
     4621 acgctgcgtg atgacggtgg tgatatggcg gatggtcttt acccggttct ttcctggctg
     4681 acctggccga tgtctgtggg taaatggacg cttgaaggga tcgaaacccg cgctcagctg
     4741 ctggattccg atggtctgct gcgtcagtcg tccgatcctt atattatggt gcgcgaagcg
     4801 tacttccagc gtcatgattt catcgctaat ggcggcgaac tcaaaccgca ggaaaatccg
     4861 aacgcacaag cgattcagga tgatttaaaa gatattgatt ctgaataaga aacaaataaa
     4921 aaaggtgagt cgcaaaactc accttttttg ttgttatccc tcaattacca caagtaaaag
     4981 gagataaacc gacaatgacc cggacgtaat gatgtaaaaa tactgatgtt cgtcactgac
     5041 tatttccgat attcaggcgt tgaaaaatca aaaaactgga aaaataattt taaatatcat
     5101 caaaattaac acctggcgca gatagttgat taatttcatt ttgatatttc gcctggcgat
     5161 tttgaagtct tttttcaacg ttatcttcga gatccttaaa gtctttgtat ttattttcag
     5221 gatggtcaac gtcaactttg ttagcgaata aattagaaat tatttctttt acattgtcgc
     5281 gtctaaattt tgcatcgtcc acttgaaaat tatatcttat tgctcgacct aaacgtttaa
     5341 aaacttcgtt aggttctctc agtctttccc cgttgagctc gtttacaacc tgatgatcgg
     5401 aacctgttgc aaatgattct gccatgatag ctttactgct ttcaaaccac gctgaagcat
     5461 tgctgaagca ttcctggctt tttcactaca atattgggtg agactatcac cattaatacg
     5521 aattcctctt tgaatgtggt agtcgtcctg atcgtagtaa ccgagagata agtaatcatt
     5581 tccgttttta tttttaatta caatagcgag agagttgcct tttttatctt taccgtacca
     5641 ggtaaataag tccgttggtt cgctattccc gctctcatca aattccgtta ccgtatgtct
     5701 gagtttgtgt gatatcttat cgaagataaa accaggatct ccatcatata tttcttttaa
     5761 tgcgctgaag atcatttcct tgttattgat atcatgagtc ttctcaacta tgttgtcatt
     5821 aatgatagta cggacgagtt ctttgtcttt atagttcgca ggtaattcta ttggttcttg
     5881 gttgtcgata ccaatagtaa tatggtctgt atcattggag ccaatatcaa tataagtaac
     5941 tgaggaatta ttaatattaa atcttaagga tcgtcgttta ctcgtatggc gttttttata
     6001 agctcaagta gatgtttaat acaggtgctt attccttcct taccattaaa aaggtttttg
     6061 agctcttgta ctaatccatt aacttcatca ttgttatttt cattaatgga ttctgggtaa
     6121 gttgcgctgg tattgttgat tgaaatagat ggtagcatga ttttattcca ttttgattga
     6181 tttgttacaa atatatatta c
//
LOCUS       DAKISA010000173         6193 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.176, whole genome shotgun sequence.
ACCESSION   DAKISA010000173 DAKISA010000000
VERSION     DAKISA010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6193
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.176"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            267..1925
                     /locus_tag="OVW45_004100"
     CDS             267..1925
                     /locus_tag="OVW45_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCV5528413.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIVTTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1922..2878)
                     /locus_tag="OVW45_004101"
     CDS             complement(1922..2878)
                     /locus_tag="OVW45_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCV5528414.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3029..4144
                     /gene="macA"
                     /locus_tag="OVW45_004102"
     CDS             3029..4144
                     /gene="macA"
                     /locus_tag="OVW45_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCV5528415.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4141..6087
                     /gene="macB"
                     /locus_tag="OVW45_004103"
     CDS             4141..6087
                     /gene="macB"
                     /locus_tag="OVW45_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCV5528416.1"
                     /translation="MTPLLELKDIRRSYPAGNEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
BASE COUNT         1446 a         1482 c         1726 g         1539 t
ORIGIN      
        1 ggttgcccca tatagtcatt tgtctgattg acagtgtagt gcacgcaaaa gatttaatcc
       61 tttaggcgta ataaaaaata atatatcatg ctaattattt gattttgttg tttttgcaga
      121 cttatcagca agagggagta taacgcgatt attcgctcat ttttcagaca tttgccatgc
      181 ttaaatgtga tgtcatcacg tattagcaag gcctttcccg ttatactgcc agcgtaaagg
      241 ataagtcaca tatttctgga ggggatatga ttcttgagcg cgttgaaatt gtgggttttc
      301 gcggtatcaa ccgtttgtcg ttgatgctgg aacaaaacaa cgtcctgatt ggggagaacg
      361 cgtggggtaa atccagcttg ctggacgcct taaccctgct gctatcgcca gaatcagatc
      421 tctaccattt tgagcgcgac gatttctggt tcccgccggg agatatcaac gggcgagaac
      481 atcatctgca tattattttg accttccgcg aatcgctgcc aggccgacat cgggttcgcc
      541 gttatcggcc gctggaagcg tgctggacgc catgcaccga tggctatcac cgtatttttt
      601 atcgtctgga aggggagagt gcggaagacg gcagcgtgat gacactgcgc agttttctcg
      661 ataaagacgg acatccgatt gatgtcgagg atattaacga tcaggcacgt catctggtgc
      721 gtttaatgcc ggtgctgcgc ttgcgtgatg cccgttttat gcgccgtatt cgtaacggca
      781 cggtgccaaa tgtccctaat gtggaagtca ccgcgcgcca gctcgatttc ctcgcccgtg
      841 agttatcctc acatccgcaa aatctctctg atgggcagat tcgtcaggga ctttccgcaa
      901 tggtacagct gcttgagcat tatttctctg agcagggggc cggacaggcg cgatatcgtt
      961 taatgcggcg gcgagccagc aatgagcaac gaagctggcg ctatctggat atcatcaacc
     1021 ggatgattga ccgacctggt gggcgctcgt atcgggttat tttgctcggc ctgtttgcta
     1081 ctttgttgca ggcaaaaggc acattgcgac tggataaaga cgcccgtcca ttgttgctga
     1141 tcgaagatcc agaaacccgt ttacacccca ttatgctttc agttgcctgg catctgttga
     1201 atcttctgcc attgcagcgc attgtcacca ccaactcggg tgagttgctt tcgttaacgc
     1261 cggtagagca tgtttgccga ctggtacgtg agtcctcgcg cgttgccgcc tggcgtctgg
     1321 ggccgagtgg cttgagtacc gaagatagcc gacgcatatc ctttcacatt cgttttaacc
     1381 gtccgtcatc gctgtttgca cgctgctggt tgctggtgga aggggaaacg gaaacctggg
     1441 ttatcaatga actggcgcgt cagtgcggac atcattttga tgccgaaggg atcaaggtca
     1501 ttgagtttgc ccagtccggg ctaaagccac tggttaaatt tgcccgccga atggggattg
     1561 aatggcatgt actggtcgat ggcgatgaag cagggaagaa atatgccgct acggtacgca
     1621 gcctgttgaa taacgatcgg gaagccgaac gagaacattt aacggcgtta ccggcgctgg
     1681 atatggaaca ttttatgtat cgccagggat tttccgatgt gttccaccgc gtggcgcaaa
     1741 tcccggaaaa tgtaccgatg aatctacgca aaattatctc gaaagcgatc catcgctctt
     1801 ccaaacccga tcttgccatt gaagtggcaa tggaggcagg acgtcgtggt gtggactccg
     1861 taccgacgct gctgaaaaaa atgttctcac gcgtgctgtg gctggcgcgc ggtcgcgcgg
     1921 attaaccgcg aaacatcgtg gccatttgtg gctgaatagc gtcgagcatc tcatagcgcc
     1981 gacggtattc agcccgtttt ttactggcga tttcggcaat ctcttttcgt gctatctgtg
     2041 ctggaaggcg gtaatggcgt tcagcatcac atacgccgcc aaccgattcc cagaaagcgt
     2101 tgtaatcagc gtggatcttg ccttctttat cgcgataacg caggctgcgg taaatatgcg
     2161 tttcattgct gacggcaata atctgctcta cctgcaaacg ttgggcaaac agacaggccg
     2221 cttccatcac gaggcgtttg ggaaatagcc cgtggcaggc tttcgtcgca ttctggattt
     2281 cctgatgtgg aatttcccat tttgcgcctt gcagtccgcc aataaacatc gttcttttcc
     2341 cctgatattc acacagggta aacgtgatct ctgccagagg aataccttcg ctgttgcgaa
     2401 acaggattgt gctgtcacct tctttatcca ttgagatcat catggtcagc tcaagcgtga
     2461 actgctcgcc gtttttgcct tccagcttcg ccagttgcag cccgggggta ttcaaatata
     2521 agctgaattc ttccgccgac atacatccgc ggagtaacgc ataatggtaa cgtaacgcct
     2581 ccagcaattg cttacggcta agattcgccg caaggtaagg gcgatgcaga cgcacaggca
     2641 gtcgcggttg gcgcgttaac aacacattga gattaggcca gtgggaaagt tcgttcatcc
     2701 actcaacgct taaacgcggc ataatcaacg agcgcagcaa aaatttctgg cgaaaactac
     2761 ggcgatgcca gaatttaccc ggccgacact gtccacgtgc cagactaaga aaaagtgaca
     2821 ggctgctgag agattcagat ggcgtaaagg tccgttcagt tagctgcgac atattcatga
     2881 aatcaatggt tatacatgac gtcgatttca ccattgcgta tcttaaccaa acatcaatag
     2941 tgtgattact aacgtaaatt ttatggtttt gttgatattt cgttgaagtt aatgacccgg
     3001 attggcatat ggagtattca gaatatttat gaaaaagcgg aaaaccgtga agaagcgtta
     3061 cgttattgcg ctggtgatag tcatcgccgg actgattacg ttatggagaa ttcttaacgc
     3121 acccgtgccg acttatcaga cactgattgt gcgccccggt gatttacagc aaagcgtgct
     3181 ggcgaccgga aagctggacg cgctgcgtaa ggttgacgtg ggcgcgcagg tcagcggtca
     3241 gttgaaaacg ctgtcggtgg cgattggcga taaagtaaaa aaagaccagc ttttaggggt
     3301 cattgatcct gaacaggctg aaaaccagat caaggaggtc gaagcaacgc tgatggagct
     3361 acgcgcgcag cggcagcagg cggaagcgga gctgaaactg gcgcgggtga cgtattcccg
     3421 tcagcaacgt ctggcacaaa cgcaggcggt ttcacagcag gatctcgaca ccgccgcgac
     3481 ggagatggct gtgaaacagg cgcaaattgg caccattgac gcgcaaatca agcgcaatca
     3541 ggcttctctc gatacggcta aaaccaatct cgattacact cgcatcgttg ccccgatggc
     3601 cggggaagtc acgcaaatca ccactctgca aggccagacg gtgattgccg cacaacaagc
     3661 accgaacatt ctgacgctgg cagatatgag caccatgctg gtaaaagcgc aggtttctga
     3721 agcggatgta atccacctga agccggggca aaaagcctgg tttacggtac ttggcgatcc
     3781 actgacgcgc tacgaggggc aaatcaagga tgtactaccg acgccggaaa aggttaacga
     3841 cgctattttc tattacgccc gttttgaagt ccccaacccc aatggtttgc tgcggctgga
     3901 tatgactgcg caagtgcata ttcagctcac cgatgtgaaa aatgtgctga cgatccctct
     3961 gtcggcgtta ggcgatccgg ttggcgataa tcgttataaa gtcaaattgt tgcgtaatgg
     4021 cgaaacacgc gagcgtgaag tgacgattgg cgcacgtaac gataccgatg ttgagattgt
     4081 caaagggctg gaagcgggcg atgaagtggt gattggtgag gccaaaccag gagctgcaca
     4141 atgacgcctt tgctcgaatt aaaggatatt cgtcgcagct atcctgccgg taatgagcag
     4201 gttgaggtgc tgaagggcat cagcctcgat atttatgcgg gtgagatggt cgcgattgtt
     4261 ggcgcttcgg gttccggtaa atcgaccctg atgaatattc tcggctgcct ggataaggcc
     4321 accagcggca cctatcgcgt agccggtcag gatgttgcca cgctggacgc cgatgcgctg
     4381 gcgcaactgc gccgcgagca tttcggcttt attttccagc gttaccattt gctttcgcat
     4441 ttaaccgccg agcagaacgt tgaagtaccc gccgtctatg ctggtcttga gcggaaacag
     4501 cgactgcttc gtgcccagga gttgctgcaa cggctggggc tggaagaccg tacagagtat
     4561 tatccggcac agctttcggg tggtcagcaa cagcgcgtca gcatcgcgcg ggcattgatg
     4621 aacggcggtc aggtaattct tgccgatgaa ccaaccggcg cactggacag ccattctggc
     4681 gaagaggtga tggcgatcct gcatcagctg cgcgatcgcg ggcatacggt gattatcgtc
     4741 acccacgatc cgcaggtcgc tgctcaggcc gagcgggtga tcgaaattcg cgacggcgaa
     4801 attgtgcgca atcctcccgc cattgaaaaa gtgaatgttg ctggcgggac ggagcctgtt
     4861 gtcaatacag tgtctggctg gcggcagttt gtcagcggtt ttaacgaggc gctgacgatg
     4921 gcatggcggg cgctggcagc gaataaaatg cgtactttac tgaccatgct ggggattatt
     4981 atcggtattg cgtcggtggt ttccattgtc gtggtgggtg acgccgccaa acaaatggtg
     5041 ctggcggata ttcgttctat tggtacgaat actattgatg tctatcccgg gaaagatttt
     5101 ggcgatgacg atccgcaata tcagcaggcg ctgaagtacg acgacttaat cgccatccaa
     5161 aaacaaccgt gggtcgcctc agccacacct gccgtctcgc aaaacctgcg cctgcgttat
     5221 aacaatgttg atgttgctgc cagtgccaat ggcgtgagcg gcgattattt taatgtctat
     5281 ggcatgacct tcagtgaagg aaacaccttt aatcaggagc agctgaacgg tcgtgcgcag
     5341 gtcgtggttc tcgacagtaa tactcgccgc cagcttttcc cccataaagc agatgtggtt
     5401 ggcgaggtga ttctggtcgg caatatgccc gccagagtca ttggtgtggc ggaagaaaaa
     5461 cagtcgatgt ttggtagcag taaagtgctg cgtgtctggc taccttacag cacgatgtcc
     5521 gggcgagtta tgggccagtc gtggcttaac tccattactg tcagggtgaa agaaggattt
     5581 gacagcgccg aggcggaaca gcaactcacg cgtttacttt cactgcgcca cggtaagaag
     5641 gatttcttta cctggaacat ggacggcgtc ttgaaaactg ttgaaaagac cacacgtact
     5701 ttacaactgt ttctgacgct ggtggcggtg atttcgctgg tggtgggtgg tattggtgta
     5761 atgaatatta tgctggtgtc agtgaccgag cggacgcggg aaattggcat tcgcatggct
     5821 gtaggtgcgc gagcaagcga tgttttgcaa cagttcctga tcgaagccgt actggtttgc
     5881 ctggtcggtg gcgcgttggg aataactctg tcactgttaa ttgctttcac cttgcagctt
     5941 ttcttacccg gctgggagat tggtttttca ccgttggcac tgctgctggc gtttctctgc
     6001 tcgacggcca ccgggatttt atttggatgg ttacccgcac gaaatgcggc acgactggat
     6061 ccagtagatg ctctggcacg agagtaattt ttgagataaa aatgccagcc gatcgggcta
     6121 atgccgatca gttaaggatc agttgaccga tccagtggct gtgtaagaat ccggaaacgc
     6181 tcacttgttt ccg
//
LOCUS       DAKISA010000174         6119 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.177, whole genome shotgun sequence.
ACCESSION   DAKISA010000174 DAKISA010000000
VERSION     DAKISA010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6119
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.177"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..903)
                     /gene="nac"
                     /locus_tag="OVW45_004104"
     CDS             complement(<1..903)
                     /gene="nac"
                     /locus_tag="OVW45_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCV5528417.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILHTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRFTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQW"
     gene            1230..1305
                     /locus_tag="OVW45_004105"
     tRNA            1230..1305
                     /locus_tag="OVW45_004105"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1263..1265,aa:Asn,seq:gtt)
     gene            complement(1361..2297)
                     /gene="ldtA"
                     /locus_tag="OVW45_004106"
                     /pseudo
     CDS             complement(1361..2297)
                     /gene="ldtA"
                     /locus_tag="OVW45_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
     gene            complement(2359..3438)
                     /gene="cobT"
                     /locus_tag="OVW45_004107"
     CDS             complement(2359..3438)
                     /gene="cobT"
                     /locus_tag="OVW45_004107"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCV5528418.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHLAEKLLLDVICYT
                     RELAKNGVTLFGVGDLGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(3450..4193)
                     /gene="cobS"
                     /locus_tag="OVW45_004108"
     CDS             complement(3450..4193)
                     /gene="cobS"
                     /locus_tag="OVW45_004108"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCV5528419.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(4190..4735)
                     /gene="cobU"
                     /locus_tag="OVW45_004109"
     CDS             complement(4190..4735)
                     /gene="cobU"
                     /locus_tag="OVW45_004109"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCV5528420.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWHTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(4869..5306)
                     /locus_tag="OVW45_004110"
     CDS             complement(4869..5306)
                     /locus_tag="OVW45_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HCV5528421.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PLDGGPVQSLSDAYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLTGPA
                     GGAVALIHYMNEICHSRETAVNDAFCRTLTRGSLAIPQCHLLS"
     regulatory      complement(5524..5699)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(5788..6119)
                     /locus_tag="OVW45_004111"
                     /pseudo
     CDS             complement(5788..6119)
                     /locus_tag="OVW45_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1618 a         1667 c         1338 g         1496 t
ORIGIN      
        1 ccactgcctt ttttccatca ctggggaact aatcactgac attaacaact ctttcactgc
       61 ctgtgcctgt ggcgataagt tcgctctggc gggtaaattt aatgacaaag agagactcat
      121 ggaaggagtg gtaatgcgtg acatccaccc atttactgcg ccacataacg aacgcgcggc
      181 cgattcgggt aatactgcaa cgcccatgcc gctggcaatc gctgcggtaa gcgtggcaat
      241 agactcaatt tcaccaatga cttttgccgt gaatcgccgt aaggaaaaag cctcatcaac
      301 acgaagtcta atagcactgt aatcactggg gagaaagagg ttcattttcg caatagcatt
      361 cacatcaacg ctttgccccg ggcaatcttg agttcctacc agaaaaagat cttctttcag
      421 caaagcctga ctggatacac cagccacagg ggaatgctca taaatcaccg ccatatcgag
      481 ttggtgattt atcaattttt cgttaagcac tgcaccacta ttttcatgaa gatagataac
      541 gatctccgga aattcagcgc gaaccgcctg taataagggc atggtgatgg atgacgcagc
      601 ggttcctggt gcaaagccaa tcgagacttg ccccgataat gcctgaccaa cgttatgcac
      661 cgccagttgg gcctgttcac actgacgtaa aatggcccgc gcatgggtat ggagaatttt
      721 tccggcgtct gttggcgtaa cgccccgctt tgtacggatc aaaagttgtt gatttaactc
      781 accttccagt gtggcaacct gctggctgag cgctggttgt gcgatatgca atacttcagc
      841 agcctgggtc aggctaccaa tatctacaat ttttacgaag tatttcaggc gtctgaagtt
      901 catgttgcct ccggttttta agaatcggcc caagtgccgc cattacttac aaccagattg
      961 caagatgctt gccagtttta ttttggcgtt gatgtacaag ctaaccaact gtcaaataag
     1021 agattatgat agattcgtca tttgctcctt taatcagctg tcgcgttccc ctgccctata
     1081 aaaggagggt atgcaccacg atggttcatt acccaataag attgaaagct caccactttg
     1141 ttgaaattga cagcaaacaa acaaaaaaat gcatttcacc ctttgacatc accatgcact
     1201 gccattaata tgcgccccgt tcacacgatt cctctgtagt tcagtcggta gaacggcgga
     1261 ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattc aaaaaagcct
     1321 gctttctagc aggctttttg ctttctaatt accaacgctc ttaaaacatc tgtcttgaac
     1381 cagaactaat ttgcacaggc attcccgatc gacgttgcaa cgcagcattt gcgcgattta
     1441 catcaacttc ttgcccgctg ataaacgccc gcaaagatgg ggttaccggc aatggcactt
     1501 ttcggtcaga ctcatattct gcacgattgc gcgacaacgg ctcatgaact tccagccagt
     1561 tcgagccatc tggttcagtg gtgtatttta ctggctggtc gataatttgc acacgcgtcc
     1621 caacaggaac attatcaaac agatatttga tatcgtcatt gcgcagacga atacagccct
     1681 gacttacccg gagcccaata ccaaaattgg cattggtacc atggatggca tacaacctgc
     1741 caatataaat cgcgtacagc cccatgggat tatcggggcc cgcaggaaca aatgcgggca
     1801 aactctcccc tcgtttcgca tattcgcgcc gagtgttcgg cgttggcgtc caggttggag
     1861 cttcttgttt acgttcaacg gtagtcaccc agttacgcgg ggtttctcgc ccagcctggc
     1921 cgataccaat aggaaagact tccacagtat tactgtctgg tgggtagtaa taaagacgca
     1981 tctcagcgac gttaaccaca atccctttac gaacagtggc gggcaaaatc agttgctgcg
     2041 gaatggtgag ttgcgagcca gacttcggca aaaaaaacat cagcgcccgg gttcgcttcc
     2101 agcatgttac ttaacccttg cccgtattgt gcggcaaaag tctccagcgg ctgggtattg
     2161 tgatcaggaa cagttacagt aagcgactgc cccactaaac ggctaccctc tggaggtaat
     2221 ggataagtta ccgccaggct attatggctg gcaaaaagca gagcaaatga gcaaaaaata
     2281 tttacacgac gcatcatgtc cctttcctaa gtcgcgaaag ctatccgtta agtatagctt
     2341 ttatcagact tttcgttttt aactgttcaa atcagaagtc gtattccccg gtagaacaat
     2401 attactggca gcaagttcgc ccatgttgtt atatatcgca caggcagctt cgatgatggg
     2461 catcgccaga gctgcgccac tcccctcacc taaacgcatc tccatattga gataaggctc
     2521 cagccccaaa tgcgacagcg caatacgcgc ccctttttct gccgacaggt gagaaggaat
     2581 gagatacggt ttgattgcag gagacatctg gcaggctgcg agcgcggcag cataagaaag
     2641 aaatccatcc agcaacacgg gtaaaccgca ggaagcagcg cctaacatca cgccagccat
     2701 tccgaccaaa tcaaatccac ccacttttgc caggacatca acgccatcct gcggatttgg
     2761 ttgattcaac gtaatcgccc ggcgcacaac atcaatttta ttagccagtt tatctgtcgg
     2821 caggtttgcg ccaatcccaa ccacttcttc aggagcccgg ccagtgattg tgctgactat
     2881 tgccgctgcc ggtgtcgtgt ttgccatccc caggtcacct acaccaaaca gcgtaacacc
     2941 gttttttgcc agctcccgcg tataacatat gacgtccaaa agcaactttt ctgccagatg
     3001 gcgactcatt gccggagctg aagcaatatt gccactacct cgagcaacac gcatgttgat
     3061 aagcccgggg ataggctcag cagtatcaat accaacatca attacgtaga cgttagcgcc
     3121 cgcttgtgct gccagcacac acacgccggt tgttccgcgg gtcatatttt cagcctgaat
     3181 ggctgtcact tcttttgggg aaatagcgac cccttcctcc cagacgccgt gatcggcaca
     3241 catgaccagt accgcttttt tgcccacatg cggaacgcca ttcaaccccg gcattcctgc
     3301 cagttgtatg gcaagcgcct ccagccttcc caggctacca acaggtttga gtaacccgtc
     3361 gatatgccgt tgtgcacgcg acatagcggc aggatcgata gcagggatcg tattcagtaa
     3421 atcggcaagt gtttgcatgt cacgtctctt tatagcagtg ccagcaggaa gaccagttca
     3481 ccaagctcta tagcagctcc cagcgtatcg ccggtttgcc cgcccagcgt acgtttgaga
     3541 agctgaccga ggatgaaaat cgccaccatc gtcaccacca tagcagccac accatgcata
     3601 cccggcaata atacggcagc aaaaatagcg gctaagccga gtgtgacgca ggtttgtcgc
     3661 ccatcaattt tgccaatgaa cacattgcca agtccttctt cccgggcata acgatgacga
     3721 tacatcaata atgcggcagt cccgcggctg accgcacatg ctgccgccag cgatgcaagg
     3781 atcggttcgc cacgcagagc caactcgctt aataccagaa tctttgccag tacaacaaaa
     3841 atcaatgcca gaccgccgtg ggtgcctaaa cggctatcac gcatgatttc caacatgcga
     3901 tcgcggctac gtgcagaaaa tacgccatca caggtatcgg cgaggccgtc caggtggaat
     3961 cccccggtca tcagcgccag cacaagtacg ctaaacagtg ccgccagtgg tacgccacac
     4021 catgcctgca gcaccatgaa gaccagcccg ctaatcgcgc caagcaataa tccaattaaa
     4081 ggaaaagtaa taataccgcg agaataatgc tcgaaatcca gtccctggga ccagcgacgc
     4141 ggtacaggca gacgcgtaat aaaagagagc atcgcccaaa ataatttact catttgattt
     4201 ttactccaat acccgaaacc accaaccata cttcatttgc cgcagcggcc agctgctgat
     4261 ttacccgccc ggcaatatca cgaaaatgtc gtgccagacg actctccggc acaatcccca
     4321 ttcccacttc gttagtcact aatacaacct ttgcgggaca acgttggcag gcagcaatca
     4381 acgactgaat ctcagcatta atcgcctgtt ccatcgcctg ataatcccat tcatcagggt
     4441 ctttatcgcc gccataatca aacaacagat tagtcaccat ggtggtaacg cattccagca
     4501 atacagcctc atgcgggtta atgtctgcat gaattaattc atcaagatgt tgccagcgct
     4561 ccactgtgtg ccagtgcgcc gggcggcctt gccgatgatg ttctatccgt gcagccatct
     4621 catcatcaag gatttgtgag gtggcgatat acagaacctg cgaagagtcc ccaataagcg
     4681 cctctgcatg gcgactcttc ccgctccgtg cgccccccgt caccaaaatc atcataccga
     4741 ctcctgattc aattgtatga tggtattcat tgaacagaac aatggatccc ccaccgttgc
     4801 tgaccgcaaa acatttacgc gattaatact gcgcgtaata taattaaaaa tccagcattc
     4861 tcaatccatt acgaaagaag atggcattgc ggaatagcca gacttcctcg tgttaacgtc
     4921 cgacaaaatg cgtcattgac agcggtttct ctggaatgac agatttcatt catataatgg
     4981 attaatgcta cagccccacc agccggacca gtcagcaaac gggcattgtg ttctgcaatc
     5041 aaaattaccg tcgttccctc agaatgagcg agccattctg gtacggatat ctcgctttca
     5101 gctgctatca atagcagcac tgaataatcg ctatacgcat ctgataacga ttgcacaggc
     5161 ccaccatcaa gaggctggaa atcttgctgc cagtcctcac caacaaaaca ggttattggc
     5221 aatatttcag ccagatgttt cgccagatcc acatctccag ataagaggca aaaaagagct
     5281 atcgattcgg gttttatcgt tttcatgcct ctcgccctcc acttaatttc ctgtaatggc
     5341 ggtctggttg ataagccatg aaacaatatt gccacacctt gaatgcagcc ctggtgcctg
     5401 tcggcactgc gatgaaagaa ctaaatgatg ccgccatacc ctgatccttt cagggtggta
     5461 ggggtataaa gacgcgccag tactctaccg gacgcttccg ggcaacccac ttcccaccga
     5521 agttgttggt attgactgac agactggtct tctggcttag cgtcatcctc acccgacctt
     5581 cccaatctcg cgatcagtgg cattttcggg tttgtcagca ttacagcagc gggggctgcg
     5641 agggattttc accctcttcc caggcacaaa tgtgccacac ctgtcagcgt atgttatgag
     5701 ctgatttcac ttttcccata acacgtaaat aacgttaatt attcattcca gcgtttattt
     5761 aaaccttccc tttttttagc atttattcta taccgtttcc atcgccgcat tttcactcac
     5821 ttgctgtcga tacacctgcg gcgtaacctg ataagtctga cggaaaactt tgcaaaaata
     5881 actgtattgt cgtggggatt aggcgcacga gtctggcgca ccgaatctga ccgagatttg
     5941 ttctctttaa taaccacaaa attgagttgt tttttgaggc aattttccac aatcaacttc
     6001 aacaaatcag cagaggccat aactcttgag taatccatct caggaacttt gcggaattca
     6061 ttaagtaatt cgggatctgc ctgccagcaa tcatcgacat tcagaatatc gaccaaatc
//
LOCUS       DAKISA010000175         6045 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.178, whole genome shotgun sequence.
ACCESSION   DAKISA010000175 DAKISA010000000
VERSION     DAKISA010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6045
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.178"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(359..1129)
                     /locus_tag="OVW45_004112"
     CDS             complement(359..1129)
                     /locus_tag="OVW45_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528422.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIYVL
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAEVVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEEEIRAVFDAVRDAVCSVSCRVAEDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDYPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSECVEHILSSGLVCDVLR
                     IPDEPSRRWFDRDILREVVLEARDEIRSMADALAKIRK"
     gene            complement(1174..1608)
                     /locus_tag="OVW45_004113"
     CDS             complement(1174..1608)
                     /locus_tag="OVW45_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HCV5528423.1"
                     /translation="MYGTCETLCRELAAKYPGDMPLMLVIWSPEEIQALADGMDISLS
                     DHEIRTVLARLEDIPEDQRTESGISSGAAMEIINNVRENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDNGLAVPECVTRRQAVVNQARALLKNNTREND"
     gene            complement(1622..1843)
                     /locus_tag="OVW45_004114"
     CDS             complement(1622..1843)
                     /locus_tag="OVW45_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528424.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LTRQTLYRANRLFMEAYTEILELDSCFKD"
     gene            complement(1844..2527)
                     /locus_tag="OVW45_004115"
     CDS             complement(1844..2527)
                     /locus_tag="OVW45_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCV5528425.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DNWFEPEAA"
     gene            complement(2604..2915)
                     /locus_tag="OVW45_004116"
     CDS             complement(2604..2915)
                     /locus_tag="OVW45_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528426.1"
                     /translation="MKYDGDGRATARFFSGKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGITLPSKRRRLRYSTVGLTRYRT
                     R"
     gene            complement(2912..3814)
                     /locus_tag="OVW45_004117"
     CDS             complement(2912..3814)
                     /locus_tag="OVW45_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HCV5528427.1"
                     /translation="MHFSGEPAKIAEVKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITEDVRFEPCPGLTAAGRGVVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPVGYEVNISSQGDNFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDNVAHYGG"
     gene            4384..5355
                     /locus_tag="OVW45_004118"
     CDS             4384..5355
                     /locus_tag="OVW45_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001760566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HCV5528428.1"
                     /translation="MIKVFCDDGSTAVKLAWFENGELKTLISHNSFKEGWNTSGFGSE
                     KTYNYLVDGEKYAFDTHNPSAIVTTNIEFQYSVENRLAVHHALLQTGIEPGDVELWVT
                     LPVSEYFTTDMQEHTDNIRKKIENLKKEITLNKGTVFSIKKVHVLPESLPAALASLEE
                     DKVSDLETSLVVDLGGTTLDCGAIAGRYKNVTQVSGEPGLGVTLVTTAAMNALKQAGT
                     HTSYYIADQLVRNRNADDLFETVVNDSSKIDTVKSVIENSISRLSDRVINHLHAYKGI
                     NRIYMTGGGAELIYPAIKSAFPLLKDKVKILPNAQLALVISIAEINR"
     gene            5367..5918
                     /locus_tag="OVW45_004119"
     CDS             5367..5918
                     /locus_tag="OVW45_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001760564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stability family protein"
                     /protein_id="HCV5528429.1"
                     /translation="MGEERRKLWLYLYPDEPQQQSAIETIEEIPQRQRSDFFRDSIVA
                     GIALSKLDKRLPALVAMLLQQDSNIDTLVRILKILFPKLNIDSTENKSTLLAVAWQKR
                     CRDDLQQGTPWSSWFAISEDEYNHHIDHPASNCQVRALWTYDENEQKKDISNQESMSF
                     SDTNINKNSETLTNAKKLFGTSD"
BASE COUNT         1572 a         1654 c         1513 g         1306 t
ORIGIN      
        1 aaagccgggc gataaggcgg ggatgcacag ggcgcaaggg aagccgcagc ctgacgcaca
       61 cggctggcgg gagttggagg gtgaaaccgc gaaatctgag cgggtgaacg accggaaaat
      121 cacggcagac gggaaggagg gaccgggtga ccgcagcggc gaagcgtacc cttgcatccc
      181 tgccagcccg gctaagatgg aggcacggca cagcccgcac acgacacggc ggccacataa
      241 ctgaaaaccc acaaggccat gccccgcatg gccgggtaaa acggagcctg cggcgacatc
      301 cttttgctct ttccggggac cggctggaaa aggcggagat ttttccccgc cagcgtcatc
      361 atttccggat tttcgccagt gcatccgcca tgctgcggat ttcatcccgt gcctccagaa
      421 caacctcccg cagaatatcc cggtcaaacc agcgacggga cggttcatcc ggtatacgca
      481 gcacatcaca gaccagacca ctggacagga tatgctccac acactcactg accagataca
      541 tttccaccgc cgaaaaacgt gctccccggt tcaggatggc atcgagcacc acgggcagat
      601 tttcgtcatc ttcatcaagc cagcaggggt aatcgctttc cttcacaacc agacgaaagc
      661 ggcgcgtctg gcgggtcagc aacgtctgac cggtggtagt cagcgccgca cggatacgcc
      721 gcgcatcttc tgccacccgg caactgacac tgcatactgc atcgcgcacc gcatcaaaaa
      781 ccgccctgat ttcttcctcc gtcagccatt caccggcata cgggaaagac acctgcgttc
      841 cgtctgccgg aagtaccggc agcgactgcc actgactgcc ggacagtaca acttccgccc
      901 cggttccggt cagtttctga atggcggagg ccaggtgcgg cggcagcacg ccatcgtggc
      961 ggttaacgag taacgtcgcc agccggggcg catccagcag aacataaatg ctgatccagc
     1021 ccggatactt cgggttacgc tggtcaacat ccgtctcttc cggccagccc tgcgccagca
     1081 gcaggttacg ggcgtggcga caaatcagtg cattcagcgt ttcgttcatg attcatggtc
     1141 tccctgaatc gccgcccgca ggcggcgata acatcagtcg ttttcgcgtg tgttgttttt
     1201 cagcagggcg cgggcctgat taaccaccgc ctgtcggcgg gtgacgcatt ccgggacggc
     1261 gaggccgtta tcacgggcgg cccactcacg tttccacagt gcctgttcgg cggtctgaat
     1321 cagggacgcc agcagttcag cagggacggt cacctggcgg ttttctctca cgttattgat
     1381 gatctccatc gccgcaccgg aagaaatacc ggattcagtc cgctggtctt ccgggatgtc
     1441 ctccaggcgc gccaggacag tcctgatttc atgatcggac agggaaatat ccattccgtc
     1501 agcgagggcc tgaatttctt ccggggacca gataaccagc atcagcggca tgtctcccgg
     1561 atactttgct gccagctccc ggcatagcgt ttcgcatgtt ccgtacattc tcgtctcctt
     1621 ctcaatcttt gaagcaggaa tccagttcca gaatttcggt atacgcctcc atgaacaggc
     1681 gattggcgcg atacagcgtc tggcgtgtca ggcgttcaac aagcacatcg gaatcaaagc
     1741 gacagcgatg ctccatatcg ttcagcctgg cccgaaggtc acacatggtg ttggccacct
     1801 ccgccacgtc ggcgcgaagt ttttcgtgtc cggcatagtt catttacgca gcctccggtt
     1861 caaaccagtt atcattcgcg gccccctgct gcatggcccg ttgcacggcg gcaaggcgtt
     1921 gctgcccggc acggtgatac tgctcaagca gctcgatacc gatataccgg cgtccggact
     1981 ggagggcggc gacgcaggtt gagccactgc ctgcaaacgg gtccagcaca attgcgttcg
     2041 ggtgtgtgaa gctctcaatc agtggttgca ggctggtaac gggcttttcc gtcgggtgat
     2101 gacgattgcc tgaatatttc cagcccagca cgtccggcag cgggttttgt ggcagacgtg
     2161 gacggccttt tgccaggatg taggcgcatt cgtggcgata gcccacatat gcggccttcg
     2221 atgtgtagtt tttggtgaag accaggtgac caacaacgct gaatcccgca tttttccagg
     2281 cggccataaa gcgatcgacg cggttccagc catagaagct caccatcagc gcatcttttt
     2341 tgagtacgcg gtacatttca ttacaggccg gttgcagcca ttcatcggtt ttatcgccgg
     2401 cgatggtgcg cccggaacgg tcacggaaac cgacgagata cggcgggtcg gtgaggatga
     2461 aatcaatggc gttatcaggg atacgggcca taacatcgat gcagttaccg aggacaaaac
     2521 gggacataac aacactcctt ctcttacaga aggcaccggg agggcccccc tgtggcgccc
     2581 gttccccgct gcctgtgggc tgttcaccgc gtccggtaac gggtcaggcc gaccgtggaa
     2641 taccgcagcc gtcgtctttt tgacggcaag gttatgccgc gaaagcggct tgaagggcgt
     2701 tcatcagaaa agggcgacgg gctggcgtac actgtcaccg acgccagccg ggagcggtaa
     2761 aagcgcccgt cccgtgcccg ccggacgcgg gacactgacc acaggcagcg tttatgccgc
     2821 gccgcgtcag ccggcgcagt aaaaagagga gcacggcgac atcctttacc ggaaaagaaa
     2881 cgggcggttg cccgcccgtc tccgtcatac ttcatccgcc ataatgcgcc acattgtcga
     2941 ctatccacgc aggtccggtg acttccggca gctcatcgtc atctgtcggg gaagaccatt
     3001 ccagttcccc ccacagcaca tcaacgagct ctccgcgctc atacaactgc cagccacaga
     3061 aatcacagcc ctgttcggca taccagtgct ccagcgtgca gctgaaacgg cggcttaatt
     3121 ctgcaataac gtcgctttcc ggctgacacc acggagtgtc gaaatcaacc tgaatgaagt
     3181 tgtctccctg actactgata ttcacctcat accccacagg ccacttcacg ccataccgtt
     3241 ccgtatacca gtgatatgca gaaggaacac cgttcagaac accaccgtta aagccattca
     3301 cttcaacatc caggcgggtc ggcagaaccg tcagcaggtc aaacggcatg gttttttccg
     3361 gcagtacgtt gtcacacaga cggttccacc acaccgatac atcctcgtta ctccagaagc
     3421 cacaccagtc acctcttttt gcggtgaaaa gtgcggtgat ggtatccctg acatcgtccg
     3481 gtaatccttc ccagcgacgc tgtccagtac cgctctgcag ccagagttca tgcagcatca
     3541 ggcagttttg ttcatccagc agtacaccgt tctgcaggtg tgtcagccag cgggtgaacg
     3601 cgatattttc cgtcgataca acgccacgtc cggcagccgt cagtccggga catggttcaa
     3661 accgcacatc ttcagtgatc tgcagaagtc cggcacttcc ggccagaaac agctgaatac
     3721 cttcatttgt ggcgcggcga taaagcggtg tgactgcacc gctggccagt cgtttaacct
     3781 cagcaatctt tgccggttca ccagaaaaat gcatacggtt actgcaccag tttggcatgg
     3841 gaattctcct ctcacttctg cttcagcaga gagcaccggg agggcgcccc tgtggcgccc
     3901 gttccccgct gcctgtgggc tgttcaccac gtccggtaac gggtcaggcc gaccgtggaa
     3961 taccgcagcc gtcgtctgtt tgacggcaag gttatgccgc gaaagcggct tgaagggcgt
     4021 tcatcagaaa agggctcttg gctggcgtac actggcaccg acgccagcca ggagcggtaa
     4081 aagcgcccgt cccgtgcctg accggacgcg ggacactgac cacaggcagc gttcctgccg
     4141 cgccgcgtca gccggcgcag taaaaagagg agcccggcga catcttttgc ctcccaacaa
     4201 aaacccaaaa caaaccccac ctacccgcca caaaaaagaa acacccataa gacacccaaa
     4261 aaaaacaaaa accaaacaca caaaacccaa aaaagaccca aagcagaata aaaacccttg
     4321 aacacggcaa ataatgctgt actatgggtg cagacaccca actaacaccc gcaggagata
     4381 tttatgatta aggtattctg tgatgatggt tctacagccg ttaagcttgc atggtttgaa
     4441 aacggcgaac taaaaacact cattagtcac aactcgttca aggaaggatg gaacacatca
     4501 ggttttggaa gtgagaaaac ctataattac ttagtagatg gtgaaaaata tgcgtttgat
     4561 acacataacc catcagcaat tgtcaccacc aatattgaat tccaatatag tgtggaaaat
     4621 cgtcttgctg tacatcacgc gctactacaa acaggtattg agccaggaga tgtagaactt
     4681 tgggttacat tacctgtatc tgaatatttc accacagata tgcaagagca tacggataac
     4741 ataagaaaaa aaatagaaaa cctgaaaaaa gaaattacgc taaataaagg caccgtattc
     4801 tcgatcaaga aggttcatgt attaccagaa tctctacctg cagctctggc ctctctcgaa
     4861 gaggataaag tcagtgatct tgaaacgtct cttgtcgttg atcttggtgg gacaactctt
     4921 gattgtggtg ctatagctgg tagatataaa aatgtaacgc aggtatccgg agaacctggc
     4981 ctcggcgtaa cactggtaac aacagcagca atgaacgcct taaaacaagc aggaacccat
     5041 acaagttact atattgcaga tcagcttgtc agaaacagaa atgctgatga tttatttgaa
     5101 actgtcgtta atgacagtag taaaatcgat actgttaaat cagtaattga aaacagtatt
     5161 tccaggcttt cagatagagt tataaatcac ttacacgctt acaaaggaat aaatcgtata
     5221 tatatgactg gtggtggtgc agaactgatc tatccagcaa taaaaagtgc attcccctta
     5281 ttaaaggata aggtgaaaat tttacctaat gcgcaattgg ctcttgttat ttctattgct
     5341 gaaataaata gataatgagg tgcaggatgg gagaggaacg tcgtaaatta tggctatact
     5401 tatatcctga tgagccacaa caacaatcgg caatagaaac cattgaggaa atacctcaaa
     5461 gacaacgttc tgatttcttt agagacagta ttgttgctgg cattgccttg tcaaaactag
     5521 ataaacgatt accggcactt gtagctatgc tactacaaca agatagtaat atagacactc
     5581 tggtacgtat tttaaaaata ctatttccta aacttaatat tgactcaaca gaaaataaat
     5641 caacactcct tgccgttgcc tggcaaaaaa gatgtcggga tgacttgcaa caaggtacac
     5701 catggtcttc ctggtttgca attagtgagg atgaatataa tcatcatatt gaccatccag
     5761 cgtcaaattg tcaggtacgt gcactatgga cttatgatga aaacgagcag aaaaaggata
     5821 tatcgaacca agagagcatg tccttttcag acacaaatat aaataaaaat tcagaaacgt
     5881 tgactaatgc taaaaagtta tttggcactt cagattaact tttaatttat gatatttgcc
     5941 ttccccatgg aaggcaaata cattaagttc ggagtgacgt aacaaggggc cgcctagaat
     6001 tcgaaaaaca aaccacgtca aaactctggt gtaatttccc cttca
//
LOCUS       DAKISA010000176         6023 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.179, whole genome shotgun sequence.
ACCESSION   DAKISA010000176 DAKISA010000000
VERSION     DAKISA010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6023
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.179"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..261
                     /locus_tag="OVW45_004120"
     CDS             <1..261
                     /locus_tag="OVW45_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCV5528430.1"
                     /translation="WRGGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTV
                     NADVNGARNILAAGHAVLACGEMVQSGRPLKQEPTEMIQATA"
     gene            complement(301..972)
                     /locus_tag="OVW45_004121"
     CDS             complement(301..972)
                     /locus_tag="OVW45_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HCV5528431.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(1079..1312)
                     /gene="yedF"
                     /locus_tag="OVW45_004122"
     CDS             complement(1079..1312)
                     /gene="yedF"
                     /locus_tag="OVW45_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCV5528432.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            complement(1309..2514)
                     /gene="yedE"
                     /locus_tag="OVW45_004123"
     CDS             complement(1309..2514)
                     /gene="yedE"
                     /locus_tag="OVW45_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCV5528433.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            2701..3114
                     /locus_tag="OVW45_004124"
     CDS             2701..3114
                     /locus_tag="OVW45_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCV5528434.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            complement(3148..4635)
                     /gene="amyA"
                     /locus_tag="OVW45_004125"
     CDS             complement(3148..4635)
                     /gene="amyA"
                     /locus_tag="OVW45_004125"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCV5528435.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(4713..5078)
                     /gene="fliT"
                     /locus_tag="OVW45_004126"
     CDS             complement(4713..5078)
                     /gene="fliT"
                     /locus_tag="OVW45_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCV5528436.1"
                     /translation="MNHAPHLYFAWQQLVDKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            complement(5078..5488)
                     /gene="fliS"
                     /locus_tag="OVW45_004127"
     CDS             complement(5078..5488)
                     /gene="fliS"
                     /locus_tag="OVW45_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCV5528437.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            complement(5503..>6023)
                     /gene="fliD"
                     /locus_tag="OVW45_004128"
     CDS             complement(5503..>6023)
                     /gene="fliD"
                     /locus_tag="OVW45_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCV5528438.1"
                     /translation="KEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFANGASDSAFK
                     TLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTKIATEVKGY
                     LADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMMSKLNNTST
                     YLSQQFTAMSNS"
BASE COUNT         1472 a         1585 c         1497 g         1469 t
ORIGIN      
        1 tggcgtggcg gtcaggtgct tgctgttccg ccagcgtaca caagccagcg ttgcgcgtgc
       61 tgtggtcata cagcgaaaga aaaccgcctg tcacaaagta aattcagatg ccaggtatgt
      121 ggatatacag tgaacgccga tgtaaatggc gctcgtaaca ttttagcggc ggggcacgcc
      181 gttcttgcct gtggagagat ggtgcagtca ggccgcccgt tgaagcagga acccaccgaa
      241 atgattcagg cgacagcctg aacgtagcag ggatcctcgt ccttcagggc gtggaggatg
      301 tcaacaaaca ggttgaatta actccgcgcc ctggtttttg acattaccta ccgcgcgcga
      361 taccgggtgc caggtgaact ggtttgctgg cacacagccg ttggtagcaa tttctgatgc
      421 ttctttaccg ccaatctctt gccgcatcca ttcctgtgca gcttctggcg acagaaccag
      481 cgggcggcgg tcatgaatgt ctaccagacc ctggtctgct gcagcagtga caattaaaaa
      541 tccttcggct tcgtcaccac gctcaaatgg tgtgctacct atcgcggcca taaaaatagg
      601 ttgtccatca gcacgataga taaaaaaagg ctgttttttg tcgccttctt ttttccactc
      661 aaaccagcca tcggcaaaac agattgcccg accatgttgc cagagcggtt taaacatgcg
      721 actggttgcg gcagtttcta cgcgggcgtt aatcagcggc ggtttatccc accatccggg
      781 agcatatccc cagaaaaccg gatccagatg aaggtgttca tcacgttcac tgagcagaag
      841 aactttggtt cccggcgcga cgttgtatct gccaatgggt tcgggatcgt agggaatatc
      901 gcgttcaata tcttccgcga gaagcgcaag gtaatcttca cgcgtttggg attgggcaaa
      961 gcgtccacac atagaaacct cctgagatca tactgaaaag aataggagag ggggcgggta
     1021 aaatctatgt gtcagaggca acaatgttcc cgctgtgggc cacagcggga atgagggatt
     1081 acttctgaat taaataacga atggtcggcc cgtcttgctg aatatccagc accgtatagc
     1141 cgtgattacg cgcatccagt ggaatattat tgatcgactg tggacagtcg ctcaccactt
     1201 ccaggatttc cccttttttt aactgcggca tcgcctcaag ggttgcgact gccggataag
     1261 ggcagggttc acccaccata tcaaggcggt aatcaggaac gatatttttc atgcgatctc
     1321 cttagcagtc tgcggtgccg cacggcggaa gaagcgtttt tcccagccga taatcaacat
     1381 cagcgcagta aacaacagca aatatgtcac cagcaggcca cccatcgggc caaaggtttt
     1441 cagcaggttg attttgtccc agtcggtggc cagcgccgga gcgaaatcat cccagtaata
     1501 cgccagaatc gtcgagccga tcacattgcc cagaccgacc caccagtagt gcacctggcc
     1561 ttctaccgcg cggtacatcc agccggtttc gcagccgcct gccagcacga tgccaaaacc
     1621 aaacagtaaa ccaccaatta ccgcgtttgg tcccgcccac atgattttgg gttcaacgcc
     1681 taactgtacg taactgaaga tcccgatggc actcaccgcc ataccgataa tgattgcttt
     1741 cgccatatgg gtacgtccgg tgatccacat atcgcggaac gccgaagtaa agcagatttg
     1801 cgcacgttca atcagtaaac caaagccgac gccaaacagc attgccagcc ccagtttggg
     1861 ttggttcatc gccgtgagca gcgcccagcc cagcatgccg aaaaagacca gcatcccgag
     1921 acgaaaacga cgccgcgcct gatccggttt ttgcgtcagc ggtgaggcgg cagaaacttt
     1981 ctgcattttc acgggaatac ggaagatggg cagcagggta aagcgcgcgc caaaccatga
     2041 accaatggca gtggcgatgg caaagaacca ggcatgcagc gagaactgag gaatgccggt
     2101 aaagaacgcc gccaggttac agcccattgc cagacgcgcg ccaaaaccgg cgataatgcc
     2161 gccaataatg gcctgcataa tgcggatacg gctgcgcggc atgcgcagtt tgacattgtt
     2221 ggcccacagc gctgcggcaa agcagccgcc aaacatgccg aggatcatca tcccgtcgat
     2281 gcgggttaat ggcgatcctt ccagatggat aattttaaaa taaccccact cttcagcatg
     2341 gacgccaaac agctgcagga gctgaccgcc ccaacgggta aattcacccg tgacagccca
     2401 aaaggtgcca gtaatgccaa aatagtaagt ggagagaata cccgccgcga tgaccgcagg
     2461 gatgggtgcc cagaatttaa tcaaccaggc gtgtttgaat tgctgccatg acatgaatgt
     2521 agcctctgaa ctcagaaggt ttgaaacaag aatcgcgaat catacatcat gcaaatttat
     2581 tttcggggta ataattcaca aaaataatag ttagatcaaa tttctctctg aacctggaaa
     2641 ggctggcgac atcagggctt taatgccaca atcctctttt cttctcatgc aggacgaatg
     2701 atgaaaaaac tcgcaattgc aggtgcgctc atgttgctgg cgggctgtgc cgaagtcgaa
     2761 aactataaca atgtcgtgaa aacccccgcg ccggactggc tggcgggcta ctggcagacc
     2821 aaagggccgc aacgtgcgct ggtcagtccg gaagcgatag gcagcctgat tgtcactaaa
     2881 gagggcgata ccctggattg ccgtcagtgg cagcgggtga ttgccgtgcc gggcaagttg
     2941 acattgatgt ctgatgatct cactaacgtt accgtaaaac gtgagctgta cgaggttgag
     3001 cgtgacggta acaccattga gtacgatggt atgacgatgg agcgtgtgga tcgcccgacg
     3061 gcagagtgtg ccgccgcgct ggataaagcg ccgttaccga cgccactgcc gtaatgaaat
     3121 ctccccggct tgccgccggg gatgaattta aatcacctct tcgataaccc acacgctgac
     3181 gctgccgccg ttgcaaaaga aggtcgcttc gccgttttcg tcggtcacta cgctctcttg
     3241 ccgattcccc agaaaatcac gccaggtttt attgccgtaa ttctctccca gatgaatggt
     3301 tttttcgcca tcatccccgt tcgacatgac caccacgcag ccgggatatt cgtcagtgcc
     3361 actgcggcta aaggcaatgc agttcggatg gtcgaaaaat aacgtctgta caccgtgggc
     3421 gaaacgctga cgggcgagaa ttaactcatc aagctgttcg attattggca tatctatcgg
     3481 ataggtttgc ccgtcaccac cgacatcttc gtaatgcgca ccgtagaggt ccggatagaa
     3541 caccgaagga acgccatttt cccgcaacaa aattagagca tacgccagcg gtttaaacca
     3601 cggttcgacc ggcgcttcga gggcttgcaa cggttgggtg tcgtgattgg ctacgagggt
     3661 tacggcgtgg aaaggatcgg cttccactaa tgtacccgtg aaaatctgcg tcatgtcgta
     3721 gtcgcgtccc atgcgcgatg cttcatggaa tttcatctgc agcggcgcat cgaacagcat
     3781 ggttttgcct tccacctgat caatatacgt ttgcagttta tcgacttcat gcgaccagta
     3841 ctctgccaca ataaacagcg gctttggcgc aacttcctgt acgtgttcga tccactcttt
     3901 ataaaaccac gccggaatat gtttaaccgc atcaagacga aaaccgtcgc attgtgtttg
     3961 ttccatcacc cagcgcgccc agtatttaat ctcttccgtc acggcatgat tgcggaaatc
     4021 gatattctca cccatcagat aatcgaaatt accgagttca tcatcaacct gatcgttcca
     4081 gccttcgccg gtgtagtcgt taacaatttt aaatatgcca tcttcatcag gattttcgat
     4141 atggtcgata ccgctaaaac atttgaagtc ccagataaac tgcgagtatt gcccggcacg
     4201 ggcggggaag gtgtaacgcg tccagccttc gcactcaatg atttcttcat caatttgcgt
     4261 acggtcatca gcatttacac gctgcacgcg aatagcttct ttttcatccg cccccatttt
     4321 gtggttgact accacgtcca gtagcaccgc aatgtcatta cgtttcagag cgtcaatggc
     4381 ggccagcagt tgtgctttat cgccatattt agtagggatg ctgcctttct gatcaaactc
     4441 acctaaatca aataaatcat aggagtcgta gccgaccgaa tacccgcccg atgcgccttt
     4501 ataggcgggc ggcaaccaga ccatattgat accaatatca ttaaaaccgt cggcgcgctc
     4561 ggccagttca ggccagagct taccgccttc cgggtaatac cagtgaaaac attgtaacag
     4621 cgtgggatta cgcattcttc gactccattc aaggggaaca ttagaagcgt agccgtaatc
     4681 ggattattcg cgagccatcg gctcgttaag attcaaaaga ggttatcctg cggagccagc
     4741 acaaagccgc cctgatctcc ataggcactt aacactgatt tttgcaccga tgactgaccg
     4801 accagtttcg ccagttcatc catacgaatc tgtaataact gctttacctt gctttcgttg
     4861 tcgagaatca ggcgcagcat cgggcggagc tgctcctgca tcgtggtgga cggggcaacc
     4921 tcttcagtca gatgtgcaat ctcctgcacc gcattcacat acgccatttc gctggcgatg
     4981 agttcgtccc attgttcttc cgttgccagg cgtaacatga gctggctttt atcgacgagt
     5041 tgttgccagg cgaaatacaa atgcggtgca tggttcatca gactgggtcc tgaatcaaag
     5101 aaggggagag taaagactct ttccaggcat cggcaatatt gcgcattaat gcttccactt
     5161 cttcgactgc ggagacatcg ttgcgtaaat tggcttgcag caagcgcctg accatatagc
     5221 tataaagagc aatcaagttt tgggttagtt cgtctttgct ctcttcatca agactcaccc
     5281 gcagcccgtt ctcaatgatg ttgatcgctt ttgacaaaga gacgcctttg ccttgctgat
     5341 tgttgtcctg cataaacagg cgcgctctaa ccagtgcgct cagcactcca tcaaatagca
     5401 tggtgaccag ctgttgctgg ctggcgctca ttacggcgct ttcgacgcca atttgtgcat
     5461 aggcttttga gccgctcgcg gtatacatgc tgacctccgt gatcaggaat tactcattgc
     5521 agtaaattgt tggctgagat aggtactggt gttattcagt ttgctcatca tggtatctaa
     5581 ttgcgtaaac tgtgcagtgt aacgcgccac ggtgtcatca atgctggcac tgacggatag
     5641 atactgtttg gtcagttttt tcagtgtggc gttaatactg tcctgtgcgc tatcaataat
     5701 gccatcatcg gcaaggtaac cttttacctc ggtggcaatt ttggtggtaa ttcctgtttc
     5761 tttaccatcg cctaccagca attcacgtac ggatgctgta ttttcattca ggactttttt
     5821 gagtttatca tcatcaattt tcagcttacc cgtcgtgccg tcctgcttaa tcccgatctc
     5881 gtttaatgtt ttgaaggcac tgtcgctggc accgtttgca aattgtgcgc ggatgccgct
     5941 ttgaattgta cgtacaacgc tgtctccgag aagtgcacca ttatttttat cttgtgcttc
     6001 agcacctggg tctacttctt tat
//
LOCUS       DAKISA010000177         6007 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.180, whole genome shotgun sequence.
ACCESSION   DAKISA010000177 DAKISA010000000
VERSION     DAKISA010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6007
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.180"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            292..368
                     /locus_tag="OVW45_004129"
     tRNA            292..368
                     /locus_tag="OVW45_004129"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:326..328,aa:Arg,seq:tct)
     gene            complement(413..1174)
                     /gene="envY"
                     /locus_tag="OVW45_004130"
     CDS             complement(413..1174)
                     /gene="envY"
                     /locus_tag="OVW45_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCV5528439.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENISYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(1357..2247)
                     /locus_tag="OVW45_004131"
     CDS             complement(1357..2247)
                     /locus_tag="OVW45_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCV5528440.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="OVW45_004132"
     CDS             complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="OVW45_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HCV5528441.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKRLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5207..5947)
                     /gene="nfrB"
                     /locus_tag="OVW45_004133"
                     /pseudo
     CDS             complement(5207..5947)
                     /gene="nfrB"
                     /locus_tag="OVW45_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family protein"
BASE COUNT         1363 a         1574 c         1566 g         1504 t
ORIGIN      
        1 ttctgatgat aggatgagta tccataatga tcaccgacgt tggtttcata gttaccagtc
       61 tcataggagc ggacaatttt ccgttagggc ggtaaattgt actttgatac atgaaaatac
      121 gggttttctt gattcaaacg cgcagcggtg tgcatttgtt tgccgctata gcgtaataaa
      181 tcagaaaatc agacgcggtc gttcacttgt tcagcaacca gatcaaaagc cattgactca
      241 gcaagggttg accgtataat tcacgcgatt acaccgcatt gcggtatcaa cgcgccctta
      301 gctcagttgg atagagcaac gaccttctaa gtcgtgggcc gcaggttcga atcctgcagg
      361 gcgcgccatt tcttcttcat ttaaccccgt cttatccgtt ccgctttgct cttcaccaca
      421 tcactttttg tcgctgtttg gcgagataat tcaacggtgt caggccgtaa aacgccttaa
      481 aaacagagat aaagtacgac gtgctgctat agccacataa ttgcgccacc tgagtgatat
      541 ttttgttatc catcaataac atctgtacgg cgtaacgcat acgacactct gtgacaatct
      601 ggctatagct gatattttcg ttttttaatt tctttttgag caggctgggg cttaaacata
      661 gcgaactggc gacaattcgc agattccagt aatgctgaat atcgctttga ataatgcggc
      721 agacgctgtc gcggacgctg ctgcgtaaga tatacatcag tagggcaata aaccgcgatt
      781 gctcaagaaa gttagacaat acggtaaaaa gcaatgcgcg cgtcaactcc gcttcgcagg
      841 gtttgctggg ttgctgcttg ctgtggttgg cggctagccg gaacacttcc ggtgtacggc
      901 tatgacaagc gataaccggg gttgccagcc gcgaccaggg ctttacacag gttaagttct
      961 tatttaaaaa caacagataa tcgttgatga tgttacggtt gatgtgggtg attaatacgt
     1021 gatttaacgt tgagagttca atgacgttat tgttgcacgc gaaggcggcc aggtagtttt
     1081 tcggaagggt aaacgtagca tggttattga cgcttacctc tacctctttt tcggtcagga
     1141 tcaccacgca aggttcactg ctgctcaatt gcatttcgca ctcctcagat atcagaaact
     1201 ccgctcaaag gatctatgct tcctgcatga gtgatcggcc cgttcgccga taacgatctt
     1261 ctttctttag cacgcttttt agcaattaat cttgatggaa ttctgacgag agcgaaagag
     1321 gtaagccagg tcgtacccga cttacctgga ggagatttaa tactcgagaa tgccgtgcgc
     1381 gacgggcaaa tagcgcagag agaaatagag agtgtcttta ccgcaagagg aacgtttcgc
     1441 taacagcgag gcgatctctg catccggttt cggtttcgct gtaaaggttt tgcctttgcc
     1501 ggcgacaaac agttcataaa caatgccgct tgctggggcg gagctttggc aatcggcgct
     1561 ggcctgtaga taacgttcac gctgttcagc ggtaagttta tccacgccgc tgccatcctg
     1621 tacccagaca ttaactccgg ttgctttaac ttgttccagc agttggcgat agccatctgg
     1681 cgacatgttt ccggcgaaaa aactactgat ataaaccggt tttgctgaaa catcgctaat
     1741 cagccgctgc gcgttgttta accatgttag caaaggctga cgggcagctt cgctgcgcca
     1801 gttcaggtcg tcaatttccg cgctgatgta ccagccatcc ggcgttatgc caggcgcggc
     1861 gctccataat ctggcttgct ggagatcggc agccagcagg cgattaagat agctttccag
     1921 cgctgcggac gactgtttct ggtgcataaa aaattccgga tcggcgttca gcccgacaat
     1981 cagtttcagg ccagcctgtt gcgcagctgc ggcacgctta aacaataacg tgcgctgttc
     2041 tggctgggta aatgcatcgc cgtaacgggt ccattgcaaa accagggtat cgaagccttg
     2101 caaacgtaac tgactcatca gcccctgcca ctgggtatcg gtaacctgac tatctcggtt
     2161 ttgtggttgc cagataatac ctttcatcgc aaaggaaaaa gggctgacca aaagcagtgt
     2221 cagcaatacg aaaatgaact tacgcattta ccagtgcact ccaatggtga gaaacgcgtt
     2281 gttgcgctct ccgttacgtt gattaatcgc cttaaaggta tgttgatact cgacgccgag
     2341 actgactttg tgcggccagg cgtcgtagtg cgtctcgccg gtccagatat tccagcggac
     2401 cccgactccg ccaagctgcg cgccctgagt gcctttatca cgatagccgt tgtcctgaac
     2461 gtgagcgtaa ggctcaatag tctgtccgtt agctaccttc tgatgccagc tgacgcgata
     2521 atctgccgtc cacgcctgaa tatcctggcg gatatattgc gccgcatcga ggtacaggtt
     2581 ttgggcaaac cagcctgaac cgttcgggtg ccattcgtcg ctgtatttgc cgccattaaa
     2641 gaatgaggcg ctggcgcgca gcatggtatc ggatgcgcca ttttgcccgt tcagcggcaa
     2701 ctgctgttcg acggcgagga aaaagatctg atcgcgcagc ggcttccagc gcagaccggt
     2761 gccggacatc ggatttttca ccggcatcat caccccgttt tctccggtat cggcaaagac
     2821 gcggctataa actgagagca ggtcgccttc cagcagcata ttgcgtccga tgcggtactc
     2881 ggcttccagt tgcccgtagc tacgatagct tttccctggc gctgcgccgc caacattatt
     2941 gttagcggta ctcattgccc cggaacgcaa gccgatagaa gaatcgaaac tgaacgtcca
     3001 gcggcgaccg acctcctcat gcaaacggcg gaaattgaag cgttgttgat tttgctctgg
     3061 ggtcagtggg gttatcagcg cctgattatc aatgtcatca atcaccagcc gggcgtagtg
     3121 ctgcgtcgca ggcatgtcat ccagacgctg gttcacgtag gccagttgtc ggatcagtgc
     3181 cggatcgtcc ggaagccttt tatgcgcctg ttcgagcatt tcccgcgact gtgcgatatc
     3241 accgctatcc cacaaggcgt aaccaagcgc tgcctggatg ttgctattat tcggttccag
     3301 ttccagcgcg gcgcgcaaat cactcaccgc ggccgggaca ttatgacgtt ggcgataaat
     3361 tgtcgcccgc gcaacgtaag cgttggcaga aggcgcaata ttgattgagc gcgtgagatc
     3421 gttcagtgcg agttccggct gaccaggaat gtaacgttgt gcatgcagcc accagtagag
     3481 ggcattgctt cccagtccac gttgttctgc ctgttgtagc cagcgatcgc gagccgcacc
     3541 atttcctgcc gcctgggcgg tattggcagc agcaagcaga tcctcattgc tcatgtcgtg
     3601 aagactgatt ttctgccagg ccgccagtgc ggtggcgtag tcctcaacct gatacgcctg
     3661 ataggctacc gcacgatgtt gccaggcgct cggttgtcgt tgttcggcct gaagccatgc
     3721 atacaacgcc acaccgggta gcgtgtcccg ataacacttt gccagacggt tccaggcggc
     3781 agcatcgtag gaaggcgaca tatcgcccag caagcgaact attgccgggc aattatctgc
     3841 aatacccggc aactgacttt gccactgacg ttgctccgcc agcggtaagg gtttcgataa
     3901 aatcgccacc ttcgccggcg ttgccaggta aggatgactt tccagcagag acgccagtcg
     3961 cgccattaaa gtctggctga cacgcgcatc gccctggaaa ggatagcgtt gcagcaataa
     4021 atcggcagct tcgcgtgact gctcgttctg catcagttgc caggtcagtt gatccaggcg
     4081 ggtaagattt gccggttctt gctgatacag caatcgtgcc agacgcagag attcagcctt
     4141 gttacgggtc gccacgctga cagcataacg ctcctcaagc atttcattgg cggggagggt
     4201 ggcgagcagt ttttgcgctg cgtcgtactg accttctttt aacagcaccg gtagcgtcgc
     4261 gccaacaaca tactggcggt tatcggcaaa ctgcaccgta taattcgcca atgcctgaac
     4321 ggggttggcg ctgtatttag ataacagata gagccaactt ttctcttgtg cgtccgtggt
     4381 aaacagcggc ttattttcat tgagataatg ctggaggcgt gctttttcgc cacgataagc
     4441 cagcgcggtc gcgtaagtaa tatatgactg aggatcggtg aagatcccct gtgattgcag
     4501 tgctaggatc cgatcgtcca gctgcccggc aagaagcacg tcaaaccact gacggcgttc
     4561 tgccgcgctt aatgtgttct gctggcgtgc ttcattgtat agcgtatctg cctgggacca
     4621 ttgtttcagg tagattgccc gttgcagcag atcggttcgc agcgtttttc cttccggcga
     4681 tgcagcaaac gtcgcatcgt tcagttgcgc tctggcgaca ggtaactgtg caagccgcag
     4741 ggcattctgc ccgacttcac tgcgacaacg cagggtcggc gcagcatcgc acgctttttg
     4801 ctgggcaagc agttcttcaa cagttgtaac gcttttcact tcaaccggaa tagccgccag
     4861 actgcgctca agtcgggcat ctcctgggtg acgtttcagt tgatcctcaa gcaacagccg
     4921 cgcccggtca tcatgaccaa aatggcgata ggcttccgca aggtataaag tcagcggaat
     4981 attatccggc acctgctggt gtatatattc aaattcgcgg atggcggttg cttcgtcgtt
     5041 atttttctgt gccttcagcg ccttatcgag acggggataa ataacaaaat ggcgataatc
     5101 gctcagcccc agctcttctg cgctggtgcc gatattgtct gcgagtgcgc tggtactcaa
     5161 taaagacgtc agcagtaaac cagaccatcc gatgacgcga ttaaggttat tctccttcat
     5221 tttcggactc cagttgcgca acctgttctg tgtttaaacc tgctttgagt aatagtgatt
     5281 gcatcgaaac ttgtaattcg cgttgaattg tcaggacgcg atccaacgtt tcctggctga
     5341 taacgccttc ggtgaccaaa aacttgccga gcggcagaga actgcgttca tggcgcaata
     5401 acaacacgtt aattgctgaa cgattaatat gaccgagcgt ggtcagtatt tcggcgaaca
     5461 ggaactgatg cggcacatat tgccgccaga tttcaccggc ctgctgttcc gtgagccact
     5521 gatgctgaac cgcattgtac aacattgccc gcggatcgtg accgcgtcgg cgtgcatacc
     5581 agtgacgtaa ccccgtgaca atttgtcccc gcagaacaat gaagtaacgc actttgcgtc
     5641 cgactttacg cgtcagggcc gccagcgaaa ccgggtcaat accatcttca ctgccgacaa
     5701 ttaactcatc attttccaga cgcagcggca gtaccgcata atgcagcgcc acggaggccg
     5761 gcatttcggc aatcagcgag gaagggatct gccaggcatc gatggattcc cacgccacgc
     5821 cgttttgctc tgccagcgcc tgtgccagct gctcggcgct aatcagcccc tgcatcagca
     5881 ttgaaccgcc caagcgtaga ccttcgacgc gattacgcag tgctgtatcg agttgttcgg
     5941 taatgactcc aacttattga tagtgtttta tgttcagata atgcccgatg actttgtcat
     6001 gcagctc
//
LOCUS       DAKISA010000178         5926 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.181, whole genome shotgun sequence.
ACCESSION   DAKISA010000178 DAKISA010000000
VERSION     DAKISA010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5926
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.181"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..206)
                     /locus_tag="OVW45_004134"
     CDS             complement(<1..206)
                     /locus_tag="OVW45_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="represses the celABCDF-ydjC operon involved in
                     carbon uptake; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HCV5528442.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERG"
     gene            complement(214..564)
                     /gene="chbA"
                     /locus_tag="OVW45_004135"
     CDS             complement(214..564)
                     /gene="chbA"
                     /locus_tag="OVW45_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCV5528443.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(615..1973)
                     /gene="chbC"
                     /locus_tag="OVW45_004136"
     CDS             complement(615..1973)
                     /gene="chbC"
                     /locus_tag="OVW45_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HCV5528444.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGAFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYVVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEEREEDIANAL
                     KF"
     gene            complement(2058..2378)
                     /gene="chbB"
                     /locus_tag="OVW45_004137"
     CDS             complement(2058..2378)
                     /gene="chbB"
                     /locus_tag="OVW45_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCV5528445.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(2677..3015)
                     /gene="osmE"
                     /locus_tag="OVW45_004138"
     CDS             complement(2677..3015)
                     /gene="osmE"
                     /locus_tag="OVW45_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCV5528446.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3217..4044
                     /gene="nadE"
                     /locus_tag="OVW45_004139"
     CDS             3217..4044
                     /gene="nadE"
                     /locus_tag="OVW45_004139"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCV5528447.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            4274..5161
                     /gene="cho"
                     /locus_tag="OVW45_004140"
     CDS             4274..5161
                     /gene="cho"
                     /locus_tag="OVW45_004140"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCV5528448.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5121..5696)
                     /gene="ves"
                     /locus_tag="OVW45_004141"
     CDS             complement(5121..5696)
                     /gene="ves"
                     /locus_tag="OVW45_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCV5528449.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
BASE COUNT         1551 a         1494 c         1381 g         1500 t
ORIGIN      
        1 ccccgttcca gtaacacgcg cttaccgtta atctcctgga aataacgccc ggttaatacc
       61 agagtaaatt cataatagtc gtgctgatgc agcccgctga tactctcagt tttgttatag
      121 ataaacacat ggaagttttt gccattaaac aactgctgtt ctcgggcagt ggcaatttcc
      181 ggcgcgttaa tcactggctg catcatcgac tccttatgcc ttcagttttt catgaagctc
      241 aattaattca gtaatcagtt cacgcgcaag catggacgtc attaaatgat cctgagcgtg
      301 gaccagcacc agactcactt tcatcttacc ttcgcccgca tcgccttcaa tcagtttcgt
      361 ctgtaccaga tgcgcttcat tcaatgccat ccgtgactga tccatcatgg cttttgctgc
      421 agcaaaatcg ccctgcttcg cctgtttcag tgccgcatac gccaggctgc gcgcttgtcc
      481 ggagttgatg ataagcccca tcaccacttc ttccagctct tcagcttccg tttgcgtatc
      541 gggaatgtta tcgagatcca tcatacatcg ttcctctcta ttcttaccgg cacgattacc
      601 cgtaccggca tcgattaaaa tttcagggcg ttagcgatat cttcttcgcg ctcttcttta
      661 tcaatcgcgt tttgtgcttt gttagccacc acaacaaagg gcagataaat taacgttgcg
      721 ataccaaggt tgaagagtgc aaccagcaat gcggcgacgc taccgttggt gttaaagaag
      781 gctcccagac cggttggcat ggtccacggt gcaatattgg tcaccggagg aatgatgccc
      841 atgtagtacg ctgcgagggt gattgccgcc agaatcggtt gtaccagtac aaacgggata
      901 aacatcaccg ggttcatgat aattggcaga ccaaacagaa tcggttcgtt aatctggaag
      961 atgccggacg gcagcgccag ttttgccacc tgacgataat cagcacggcg agaggcgata
     1021 aagatagcca ggatcaggcc taaagtcgca ccactgcccc caaggaaaat aaaggagtcc
     1081 agcatcggct tggcccagat atggaaggtc ttaccggctg ccagcgccgc ttcgacggaa
     1141 ccatattgct gataggtcgc gatattttcc agcgcccacg gcgtcataat gccgttgtcc
     1201 agtgcggtca gcgccagcgc gccatgaata ccgaagaacc agagcagtgg aacaaagatc
     1261 acataggccc agcccaccac gctacccaac gatgccagtg gggttgagat ggtatccata
     1321 atgatctgat ggaagttggt gccccaggta ttcaacgccc aggcaataat ccccatcacg
     1381 gaaagaataa taaagccggg aattaatgcc gagaaggaac gcgatactga agcaggtacg
     1441 ctatcgggca atttaatgac ccaattgcgg cggacaataa aggtaaacat ttctgccacc
     1501 accaggccaa taataatccc ggagatgata ttcgctccac ctaaccagtt tgcgccaacc
     1561 acataggcct caccgacact atatggggtg acggtcataa atgctgcaac ggataacaac
     1621 ccagccgcca gcgcatcgac tttacgctct tctgccagcg ccatgccaat aaagaacggt
     1681 gccattaaag acattatccc taatgttccg ttatatacgt tgccgccaat acctttcagg
     1741 ccattaagtg tttcaatggt tgaggcgtcg aggcgaatac ctaaggaata aaaaaacgcc
     1801 ccctccccaa agcttagaaa aacgttgtta attaatacaa acatggcccc cgcaagggtt
     1861 aacggcatta agcgaataaa gccatttttg attgcattaa cgtgtggctg ctttcctatt
     1921 ttaactgcaa aagggaggag taccttttca agcgatgcaa taacattact catagaaaaa
     1981 tacccttaaa aaccgcaatt taaatattgc ggtattgatt tatgaaataa ctctttgacg
     2041 ggaaaattta aaataaacta atttgctgcg gcttttttaa tcgctgcaac cgcagcctta
     2101 agcacgccta aaccatcgac tttgccataa agcagcgagt caattacttc aaccggtttg
     2161 ttgggtaaca aacgctggat ttcgggcaac atataagcaa tctgcggccc taataacacg
     2221 acatcggcat tctgaccttt ttcaccagcc agtgtttccg gaaatgcttc aataatgacc
     2281 ggaacttcat atttttctgc ctgtgcgcgc atttttgata ccagtaaaga ggtagacatg
     2341 cccgcagaac aaaacagata aatgtgtttc ttttccataa aactgccctc atcgacgatt
     2401 atctgtcagc cagacactcc gcaagcctta acctgcttcc atgctctggg taacttgcga
     2461 aaccaacatg actttttttg tggctgaaag gagtatacgg tatagacctg agcaatagta
     2521 tttctttgac cttataaatt gtttctcctt gacctgtggt tatgaccctc ttcacatttc
     2581 gggcaaataa ttcgcgaaga taattaagaa ctcaggccaa aaaaaaccgg cgcaatggcc
     2641 ggtttccgtt gttactcaag ctttcagacg aattgattac ttcgcagcct gtggatcagt
     2701 gtcgtattca gcacaggtct gataaccgga gttgatgaca tgtccggtat catctaacgc
     2761 gacaaagtag gtttctgctt taccatcacg ttgacccagg atgtaggtct ggcaagtacc
     2821 acgagcatgg atcatgctca cttcagacga aggtttaccc gcaatttgtg caacctgcgc
     2881 ccggctcatg ccttttttga cgtctttcac cacaggctgt acaaactggt ctttagtacg
     2941 atcataagcc gtacaacccg ccagcatggt taataccgcc gctgcactca gaattcctgc
     3001 catattcttg ttcatattcc gtcctcttgt ttatcagcgt gttagataag cctggaatac
     3061 attgggcgct ttttcaagcc cgcgaacgaa acggctccgc tttcagagga ttcctgtatg
     3121 acgttttaac caccattcag cccgctgtcg cttgtcgttt cagtggcaac gggttagctt
     3181 taaggaagtt ttgtcttttc tgtctggagg ggttcaatga cattgcaaca acaaataata
     3241 aaggcgctgg gcgcaaaacc gcagattaat gctgaagagg aaattcgtcg tagtatcgat
     3301 tttctgaaaa gctacctgca aacttatccg ttcattaaat cactggtgct cgggatcagc
     3361 ggcggtcagg actccacgct tgccggaaag ctgtgccaga tggcgattaa tgagctgcgc
     3421 ctggaaaccg gcaacgaatc actgcaattt attgccgtac gcctgcccta tggtgttcag
     3481 gccgacgaac aagattgcca ggatgccatt gcctttattc aaccggatcg cgtattaacc
     3541 gttaatatca agggcgcggt attggccagc gaacaggcat tgcgggaagc aggcattgaa
     3601 ctgagcgatt ttgtccgtgg caatgaaaaa gcgcgtgagc ggatgaaagc acaatatagc
     3661 attgcgggta tgaccagcgg tgtcgtggtg ggcaccgatc atgcagcaga agccattacc
     3721 ggattcttca ctaaatatgg tgacggcggt acagacatta atccgctgta tcgtctcaac
     3781 aaacgtcagg gtaaacagtt actggcggca ttaggttgcc cggaacacct ttataagaaa
     3841 gcgccaacgg ccgatctgga agatgatcgc ccttctctgc cagatgaagt ggcactcggc
     3901 gtgacctatg acaatatcga cgactatctg gaagggaaaa acgtacctga acaggtcgcc
     3961 agaacaatag agaactggta tctgaaaacc gaacataaac gccgtccgcc aattaccgtt
     4021 ttcgatgatt tctggaaaaa gtaataattt gcacataatg gtttgtgcga aaaagagtaa
     4081 tttgttcacg ccggatgcgg cgtgaacgcc ttattcgacc tacaaaacta tgcaaattca
     4141 atatattgca ggagtcgaat aggcctgaca ggcgtagcac gtcagacggt gtaacctttg
     4201 tcatcgaccc gcctcttttt taatcgctcc ccgcctgtta cactggatag ataaccagca
     4261 ttcggagtca acagtggtac ggcgtttaac ttctccgcgg ctcgaatttg aagctgcggc
     4321 aatttatgaa tatcccgaac atttacgttc attccttaat gacttaccca cccgaccagg
     4381 ggtgtatctg tttcatggcg aaagtgacac catgccgctc tatatcggca aaagcgttaa
     4441 tatccgcagt cgggtgcttt ctcatttacg caccccggat gaagccgcca tgctacggca
     4501 atcccggcga attagctgga tctgtaccgc aggcgaaatc ggcgctctgc tccttgaagc
     4561 gcgattaatc aaagaacaac agccgctgtt taataaacgg ttgcgccgca atcgccagct
     4621 ctgtgccctg caattaaatg aaaagcgcgt cgatgtggtg tatgccaaag aggtggattt
     4681 ttcacgagcc cccaacctgt ttggcctgtt tgccaatagg cgcgcagctt tgcaagcatt
     4741 gcagaccatc gctgatgaac aaaaactttg ttatggcctg ctgggactgg aaccgttaag
     4801 tcgcggtcgt gcatgttttc gttcagcgct aaaacgttgc gccggagcat gctgcggtaa
     4861 agagagccat gaggaacatg cgctacgctt gcgccagtct ctggagcgtt tgcgggtggt
     4921 gtgttggcct tggcaaggtg cggtggcact gaaagaacag cacccggaaa tgactcaata
     4981 tcatattatt caaaactggc tgtggctggg ggcggttaat tcgctggaag aagcgacaac
     5041 gttaattcgg acacccgccg ggtttgatca cgacggttat aaaattcttt gtaagccgct
     5101 gctttccggt aactatgaaa ttactgaact tgatccggcg aatgaccagc gagccagttg
     5161 atttcactga acaacagttt accttgcggt tgtaaaagcc gcagggtgtg cctgccatcg
     5221 aaccagcacg cgccctggtc ggtagtgagc aatttatcgc ccagttgcca cgccccgtta
     5281 atcacaaaca ccaccccgcc gcgtgagcca aaggtagtaa acgtacgttc ggcaattctg
     5341 actttcgcct tacagacatc caggcgtgtc atgatgttga aatccatcga catctgccct
     5401 tccgtcagtt tcgcttttac cacctggtcc gctgcaaagg caaaaggctg taacggtttt
     5461 aaggtatggt taaagcggtc tgcgctttca aggagcatct cgccgccttc cagcaacgtc
     5521 actatccttt ccatgccggg aaacaaagaa aactccccat tcgccgcgat ggatgcaatg
     5581 ctggcacgcc agtaaaaatc acgttttgcc ggtgggaaag tgcaaatttc gcgcgtttca
     5641 ccggcagcat ttcgccacag attcaccgac attttacgca tatcaaagta ttccatactc
     5701 gctcccggcc cgttcttttc attgttatcg ttcaggtaac cgtggatggt tatcggcaac
     5761 tcgggtgatt tacgttagtg gtgatcaggc tttttttaca actgtttgag aagagaaaac
     5821 cgccgatcct gtccaccgca ttactgcaag gtagtggaca agaccggcgg tcttaagttt
     5881 tttggctgaa agattattca gcagttgcag gcattttacc ttttgc
//
LOCUS       DAKISA010000179         5864 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.182, whole genome shotgun sequence.
ACCESSION   DAKISA010000179 DAKISA010000000
VERSION     DAKISA010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5864
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.182"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..559)
                     /locus_tag="OVW45_004142"
     CDS             complement(<1..559)
                     /locus_tag="OVW45_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528450.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFI"
     gene            complement(703..1461)
                     /gene="aroD"
                     /locus_tag="OVW45_004143"
     CDS             complement(703..1461)
                     /gene="aroD"
                     /locus_tag="OVW45_004143"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCV5528451.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(1492..2358)
                     /gene="ydiB"
                     /locus_tag="OVW45_004144"
     CDS             complement(1492..2358)
                     /gene="ydiB"
                     /locus_tag="OVW45_004144"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCV5528452.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEVLASADILTNGTKVGMNPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(2370..3635)
                     /locus_tag="OVW45_004145"
     CDS             complement(2370..3635)
                     /locus_tag="OVW45_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCV5528453.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFSRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3862..5076)
                     /locus_tag="OVW45_004146"
     CDS             complement(3862..5076)
                     /locus_tag="OVW45_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCV5528454.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(5176..5532)
                     /locus_tag="OVW45_004147"
     CDS             complement(5176..5532)
                     /locus_tag="OVW45_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HCV5528455.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(5668..5775)
                     /gene="rprA"
                     /locus_tag="OVW45_004148"
     ncRNA           complement(5668..5775)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OVW45_004148"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
BASE COUNT         1690 a         1339 c         1330 g         1505 t
ORIGIN      
        1 gaatgaaggc gatatctggc gcaatcgctt tgtaatagag atattctttg ttatcaaact
       61 cgaccagttt aatcaggtct tctttcgtga cttcattcag tttgccgcct tgctggcgtg
      121 gatcgacaaa tgtcccgatg ccaatatcac taataatacc aggctggtgg gctgcggcgg
      181 cgcgtaaggt ttgtgtaagt acaccttgtg gatagttata agcaataatt ttattttgtt
      241 ctgcgagttc agaaatacgc ggcgattgtc cccagtgacc acataatgcc catttcacca
      301 gaccttcttg cgccagagga ctaataccac ggtcggcgcg atcgccaagc cctgtcggac
      361 taataatcga taaattacgt ggcgtttgag tctgtttata tttatcagca agagctgtaa
      421 ttaacgtggt ggcttccaga ataccgccgc cagcgcctaa cacacaaagt gttgcttcgt
      481 cgggaatata attcaccgct tcctgtgccg acaggaccgg cactcgtcca ttaatacgag
      541 gtggttttac aggtttcatt gttaattcct gattcagatg tgattttccc tacgccagat
      601 ggagaattag cgcacagaga ctcacgaaat atttttatta aatacggtta aatatatttt
      661 ttaagttcgg cggggagggt gttcccgccg aaatattatt gcttatgcct ggtgtaaaat
      721 agttaatacc gtgcgtaaat catttaccga gatttgccct ggtgcagagg ctttttttac
      781 cgcaccaaaa gttgccgccg agccaaatac ttcaccagcc agacgagaaa ttacgccagt
      841 ttttgccatc gacatcgtga tgattggacg atcggcatac tgctcctgca tctccagggt
      901 cgcggtaagc aacgtcagca catcgctggt actttgcggc atcagcgcaa tcttaggaat
      961 atcggcgtcg aaggactgca ttttgcgcag acgggcaatg atttcttcgg cttccggcgt
     1021 tttatggaag tcatggttgg acatgacaac tttcacatca tgcgcgtggg cgtaggcgac
     1081 ggtttcttta acctgatcat cgccggtaaa taactccaga tcgatcatat caaccaggcc
     1141 gctgtcgatg gctgcacgat tgagagcaat ataagcctcg gtggaaatcg cctgctcgcc
     1201 gccttctttg gcactgcgga aggtaaacag cagcggtttt tctggcatgg tttcacggag
     1261 aatttttgcc gccgccatga cagactccac attggagagg tcggcaaagt ggtccacacg
     1321 ccattccaga atatcaaagt ccgcttcacg ataggcgaga gcttcggatt tcacgcgggc
     1381 gatatctttc gccatcagcg agacgatgat tttaggtgcg cccgcaccaa tgacgagatc
     1441 ttttacagtt acggttttca ttttttaccc tttctgcacg cggtcagcct gtcaggcacc
     1501 gaaccccatg acctgtttaa catattccag agggaaatct ttgccagtcc ataacgtgaa
     1561 ctgttcagcc ccttgccaca acaacatgcc gtatccatca atcgttttgc aacctgcttg
     1621 ttgtgcctgc tgcaataact tcgtcatatg cgggttatac acgcattcag tgaccagcag
     1681 tcccggatgt aacagactga tatcattaac caatgattca ttctcaaggg ggttcatacc
     1741 tacttttgtg ccattggtta aaatgtcggc ggaagccagg acttcagcaa aggcttgctg
     1801 atcggcgaga tcggtaaccg tgacgacaca atcggtgttt tcgttaaccc gctgcgcgaa
     1861 ggcgagggct ttatcgaaga actcatcccg acggttaaag agtttaattt cttttaaacc
     1921 ttcaattgcc ccctgcgcgc caatcgccgt tgaggcacct ccggccccta acagcaccat
     1981 cgttttgcct ttgatatcaa aaccgctctc tttaatggcg cgaatatggc ccgtgccgtc
     2041 ggtgttatag ccacgcagat agccatcatc attaacgatg gtgttgatgg cacccaccag
     2101 tttggctgct ggcgttaatt catcaacata ttcacacgcc agttgtttgt tcggcatcga
     2161 cacaccagtt ccgcgcattt tgagggcttt taatccttca attgctccgg gaaagctatc
     2221 gttatccact tcgaaggcca tataggtaaa tggcaatccc gctttttcta aggctttatt
     2281 ctgcatttca ggcgataaac tgtggcggat aggataagcc atcaacccaa tcaattcgta
     2341 ttttgcggta acatccataa ttaactcctt taacctctat gcttaattgt gtttaaccgg
     2401 gtgctaaatt tgcgttcgcc aaaccgcaca tcattctcag gaataataaa aacgcggtaa
     2461 tagcggctaa aaacaataat tgcggtaatc agggccagca gcgcgaaagt aaaatcgaga
     2521 acaatgatat attgcaggcc gatgttcgac agataaccgg taatcagcgg aataacaaag
     2581 ttagccagtc cacccatcat catataaata ctggtgactt tggctttgct tttggggaaa
     2641 aactctgaca tgaccgaaac accgagctgt aaaatgccgc cagctgctga gaaaccgata
     2701 acaaaggcgc cggcattaca caccagtgga gaagggtaca ggtagataat ggcagctgtt
     2761 attgttgcca gcgcagagtt aaatacatta gcccagatgg gccggaccat ttttttcagt
     2821 agtgcggcaa aaataaagac acagaccaac gagcccatac tgtaataaga gatggttttt
     2881 aatgcctcag cttctgacat accagcaaaa gccatcgcat atttgggcat ccacaccaca
     2941 atcacataaa aggtcgagaa tgcggctaca ccgaacagta ccgatgaaac accttccagc
     3001 cagactaacg gtttgctgtt catttgcggt aattcattag ctacgctggc gtccaccaac
     3061 tggctgggga atttgctttt caacagcatc agcgtgatca gtacaaacag aatacccgga
     3121 ataatcagcc catagccgta ccagatatta ttgagcaaca tatagctcac cagcattggg
     3181 tagaacattt gcccaaatga caccatcgct ttaaccagta tgaccgccga accagaggct
     3241 ttcggaaagc attccatgag cgcggggtag ccacccgtat ccagcgctga gttagcgata
     3301 cctacgcaca ctgccagacc gtaggcgaga gttaaattcg ggcaagcggg aataccaaag
     3361 aagaatagca gatacattat tactgccatt aatatcaccg cccgacgacc aaacttatcg
     3421 gagatcacac cgaagaataa aatactgatc aatcgcccca aaccgatacc ggaaattaaa
     3481 taggcaatgc ccgcgttgtc agtggaaaac ttttctgcca gagatgtcat attttgggca
     3541 agcgtaataa cactaatgcc gtgcaggaaa tagctgaagt aaatacaaag aacagccagg
     3601 ataaatggcg tgctgaaagc cttattttga gacattttca cagctccttg ctgaaagata
     3661 atttcgttac cgtacgtatt gttctccgac ggtttacata attactatac tgaagatgat
     3721 tttccctggg tacatggcat aaagtttatg tttccaggaa tattgcatgt catagtttgt
     3781 ttttgatcgc gattttcctt agttagcgca gaaagagtta tttatccgta agatcttgca
     3841 tgacctgaaa agaatattgc attacccacc cggagcgaca ttttccttta gcgagtgatg
     3901 ccgcgttttt ttgcggctgc gtaagccgat aaacagtgcc agtaaaaaac cgatggcagc
     3961 gatggcggta tcgaaccaca taatatcggc aatacttctt tgggacagat gagccgtaat
     4021 caacggaata gtaaaggtcg caatactgcc cgcactgtaa tagatccctg tagctttacc
     4081 tttagcgtaa gggaaacgtt cagccattaa cgtcaggcca atttgcacaa cacctccagc
     4141 agaggtaaaa ccaatgacaa aagcaaatat tatcaccaca taaaatgtgg gatgcaggca
     4201 gacggtaaac agagcaataa atgagataaa ggtgtacagc atcagtaatg ttgttgggcg
     4261 aacggtatta cgaatgagtg gagcggtaat aaatacacaa agcagcgaac ccacggtgta
     4321 gatactgagt agtttgatcg acatagtgta tgacatgcct gcaacaaatt gtccgtactg
     4381 tgccagccac tggctaacca gataaaacgt tgccattgag atatagccat ataaggtata
     4441 actggctaaa tcgataattg aacagcgatg ttccgtagag ctggtggttt tctttatgac
     4501 aggtaagcga cgacccggat ggggtgggaa cgtacaacgg tataaaaaca gagcgttaat
     4561 aaacataatg cctgcagcaa tcataaagga ccaaccgaac cacagttcag cccacactaa
     4621 caggctaatg attagcggta ataaaaattg tccgctggaa acaaatgctt taattaaaat
     4681 attggctgtc ccaggtgagc gtggaaaagc ttccatcaaa ctgggataag tgcctgcatc
     4741 gagaaaactg tttgccattc ccgccagaaa gccaaaaaca taagcgataa tgatgttatt
     4801 ggtctgcagg atgccaaaaa agaaggccat atagcagcac atcccgagca tgataaaagg
     4861 gcggcgacca aagcgatcgg ataataatcc tgcaaaaagc aagacactta atcgaccaat
     4921 gcccagcgat gagataacta tcgagacacc cgcggcatta gtctgccaaa gtgtctccag
     4981 cgaggccata ttcaggctca tcaaaatgac gcccatacca tgcaccaggt aattaaaata
     5041 caacccaagt gcggtagggt aatagggatt tttcataggt tctacttagc aaaggtgctt
     5101 taaaaagtgt agcgatgttg tgtcagtatc cattcattat tgttgtaaca acgacaattc
     5161 ctttctgtgg aaaaattaac aaagccgctc cagatcgtgt gcatataatt cagcggctac
     5221 cgattgatag atcttccagt caataaaatt gccatcaggg tagacttgtt gaaacgcggt
     5281 taagtcgggg aaaaaacgca tggtgttgtt gccgatgcgg ttatttattt tctctatgat
     5341 ggcatgaaga tgtttttttc gttgctgacg catagccaac agttgctcga ctacacaatc
     5401 aggaatggcg catttgccat tttcccactg acgccagctt tcactgtcac cggtctgggc
     5461 aatccaggtc gcgcattcat caatagtcat ggcaaaaata tggcggagtg cctggagttc
     5521 ataagcgttc atcgtgtaat ggctgcatga aataaacaat aaaatataaa taacattatc
     5581 agcctgctga cggcttgaag agagtcacag tatcttgtgc aacattattg gaaagagtga
     5641 ggggcgaggt agcgaagcgg aaaaatgtta aaaaaaagcc catcgtggga gatgggcaaa
     5701 gactacacac agcaattcgt tgtttcactc aggggatttc catgcttata aatcaatatg
     5761 ttgatttata accgtgagct aatagtaggc atacggactt tttgcgtcga tcagattcgt
     5821 ctcattagtt taaaaactgt aaagaatttg cgacaggtac tacc
//
LOCUS       DAKISA010000180         5688 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.183, whole genome shotgun sequence.
ACCESSION   DAKISA010000180 DAKISA010000000
VERSION     DAKISA010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5688
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.183"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..3577)
                     /gene="sigA"
                     /locus_tag="OVW45_004149"
     CDS             complement(<1..3577)
                     /gene="sigA"
                     /locus_tag="OVW45_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HCV5528456.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFLSSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGELTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASSGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTG"
     gene            complement(3938..4942)
                     /locus_tag="OVW45_004150"
                     /pseudo
     CDS             complement(3938..4942)
                     /locus_tag="OVW45_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            4927..5304
                     /locus_tag="OVW45_004151"
                     /pseudo
     CDS             4927..5304
                     /locus_tag="OVW45_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004902302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5343..>5688)
                     /locus_tag="OVW45_004152"
     CDS             complement(5343..>5688)
                     /locus_tag="OVW45_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCV5528457.1"
                     /translation="VPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGAW
                     LIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIAL
                     AAQSSFSQQKQN"
BASE COUNT         1395 a         1274 c         1274 g         1745 t
ORIGIN      
        1 caccggtacg cccaatcagc ggattaaagt ccttatcctt catggtcacg ctcattccgc
       61 gatcctgcca gtcgaaccgt ttacctgata cggccccata aaccagttct gcctgcggct
      121 caatccagga gtcttctgtt acatgatagc ggtagcctgc ttcagcaccc acatacaagg
      181 aatgagaact gtagtctttt gttccgagtc cagcaaaggt cgccgagtac tcattatcat
      241 ggtgaacata cttactaatc aggtcgatat aggcaccaga gtcaaatata gcggaagcat
      301 acagacctgc cccgacggac ttcgtcttgc cactgaatgc attactgctg gcatgactgt
      361 cggtatacgt catagtcaga ccagtgaaaa ggtcaagtcc atccagctca tgttttttat
      421 ccacaccaat ctgcacatgg gtgtagttgt cactgtatcc actagaagct gaacctgctc
      481 cgctcatgat gcgggcccat gcaccggcct cgccattggt gtcacgcaga tcccccattc
      541 gtttattcag gttgttgact tctgtcagga aggctttgta attaccggac atatacgatt
      601 ttgctgcacc ggaagatgct gtattttcag ccaccttata tccggtcagg gtccacgtgg
      661 tatgttcctg atcctctttc gtgctgatca caggagatat gttgctgaaa cctatggcct
      721 gagtactggc tttaaatatc tctttattgc ttccttttgg ggctgttatc aggtcaacgt
      781 ttaatcctga gttattattt cccactttct gtatatcgac cgacagactg ttattgcttc
      841 cggttaactg atttttaaca atgagtttat ccgattgatt tacagactgg ctcagatcag
      901 cccgcataat cacagcagta ttagtggcat ccagtgtgtt tactgtcaca gtatggaaac
      961 ccgaattctc tatgttacta aacaacaaaa gactgtcgcg ggtttttaat gattgcaggg
     1021 ctgactgtcc gtttactctc cataaggtgt tatacatgct ggctgaggca gcaggagcat
     1081 caatacctcc ggtataagaa gtatcaaagc cacccagcaa acctgtagcc agcggtgtgt
     1141 gtgtggtttt gtccaggtta tcttctttat actggtcaac accaaacaga atagatgctt
     1201 ttttgtctga ttttatatta ccattgaccc aggcataatt ttgagccttt aacgtggcac
     1261 catcttctgt catattatat ccatctgaca tatagattgc cgggctgtat ttattatttt
     1321 ctgtaggggt gcctgttaat gtcagggttc cggccagttc aatagcttta tctacataca
     1381 tttttccctg ggcggtaaca gtagctcctt tgttgataat caggtctgta ttaattaagg
     1441 ataatgcacc agtcatagta acagttgatt tatcaccaac agttattttg ctgttttgag
     1501 cactcagact gccgttgaaa ctactttcgc ctgctgaatc cccggagcga agctgttgtc
     1561 tgaatccaaa accttcgcca gtaatgtttc ttcctgagaa catgtcaatg tatgcagttt
     1621 tatttcccag aacaatttga gagtttttag cctctatgtg tccttttaca ttagcatccc
     1681 ggcctatact cagggttgcg ttttctaaat ttaatttccg gaaattaaat ttacgacttt
     1741 cccagtcggg ctgggaaaaa gaggctatct gattgttgga cttatattcc gtattgtaac
     1801 gtttatttac agtgctttcc tggtccttta tggcagcaga atagtctttt tgacataaaa
     1861 aaggaattgg acagttgtta ttgccttctt taaatatggc atgttctgtc gcatgtcctt
     1921 gtaatgttaa ctgtgcattc cggacagaga tatcgttttt gatatcgact gagccatcaa
     1981 agatcagatt ggcattattg ttatgttgct gggtagtatt gataatatga tttatgctta
     2041 tattacctga tacattccca tggtacatat atgcatcagt attagtaagt gataatgttg
     2101 attgcttcac gtttgagttc gttacggttg ttcccgcatc tgttgctgcg attttctgaa
     2161 atgactggtc atagccgttt agatccagtg ttccgccatt tctggtaaaa tagatttcgc
     2221 cattaccgct ttcgcttaaa gcgtctttgg catttatttt taccgtacct ttacctccgg
     2281 ccatgtaaat tttattgaag gctttttcag cactaaggat gacagtccca ttacctattt
     2341 taaggttatt tccctgtgct atttttacat ccagagtacc actacctatt ttatgcagat
     2401 tatctccggc aacgccctta acattccatt caacggtggt attattatca atatctatcc
     2461 cggcaccttt ataagactta tcttttccgt acactttata tgtctggttc ttatcgaata
     2521 taagcccgcc aatgccaaga tcggtatcct gcttgagcgt taattcccct cctcctgaga
     2581 cagacaggtc cttatctttt ataatcgatg ataactcttg ttttttacca tccagctgca
     2641 tagtgtttgt gaaattcttt tttaaattgt ttaccagaat attattaaat aacgagtagt
     2701 ttgtccaggc tatattggta ctactgagaa aaccaactgt actggtaaca ccatggaaaa
     2761 cccatttttg ttgttcttta tcatatagat aagaaccaga accactatcg ccaaaaattg
     2821 gtgagttttt aaaaggggtt ctgttggtat aaatgtaact cttgtttttc cagtccagat
     2881 taaaaagact tgcgcttaac aagtcgggat tatagttttg tccaaacaga tattgtttgc
     2941 cgtttaatat cgttgaggtt gttccggcac ctgctctgaa gccaattatt tgttccttac
     3001 cgttgtagtt tacgccatat cttttcatcg catcttcggg agaaagtgag aagtcggcac
     3061 tctccgtaac ccctgttgtt tccacgacga atttattcag acgatgaacc gaaaagtcgc
     3121 cagatgatac tctgttactt gctttatatg ctgtctgatc ccatgactgt gttgttattg
     3181 catgatgctg tgtaccgtta tgagtcgcag taacactgta cgcacctcca attgatgttg
     3241 ttgcaccttt gttggaaaca gcagaaaaat caggtacagg tagttctgga aaatgaaata
     3301 tctttccatc tttcatcatt aattgaacat ttgttgcacc cggtctgaac tctcctctgt
     3361 tttgagcaag gtcaagataa tcgcgggtcc atatattatt tatatccagc attgctgcaa
     3421 atgctgtttc gccataactt ccatatattg aggataaaat aataccgagg ataactttct
     3481 ttcttgatgt accgacacta actttccggg tcagttcaga aacagcaact aatccacctg
     3541 taatatgact atatttcagt gaataaattt tattcatacg tgaactccga tatttctgag
     3601 ttgtggttat gggatagcat ttaattacaa cggggaacta tcagtcatgg tacgtaactg
     3661 ataagggtat aatgataatc actgtcagtt atatttgtat gacagatgta acaaaatgaa
     3721 aaaaaagata agcacaagtg attgattggt cagatattga tcacttggtg tttgtatttt
     3781 gaactttgcg atcaaaattt gatctttgtt gtttccggtc aatcctttac aggttttctg
     3841 tattatccgg gggagttaag tctgtcgatg tccgacagac ttttgatgat ggaaacaaca
     3901 tgcagcgata atgtgcatgc tgttttgcag gtggaactca gaaggtgaac tggaactgtg
     3961 cgaccgcacc gtaagtgttg tccgtgccga tcaggcctgc aacatcaagg tgaaccacat
     4021 caaacggtga gatccccacc ccggccgtga atgcactgcc gtcgttaccg gccagattct
     4081 gacggtaacc ggcgcgcagc tgtgcccatt tccaggcatt aaattctgcg ccaatggcgg
     4141 caaactgacg gttgctgtcg gaggtgaaac cgctggccgg ggtcagatcc acatcaaatg
     4201 cggtggtgaa catcgcgttg tgccaggaga caccggccgt cacctgcggg cggatcctga
     4261 aggtttccgt gataccgttc acttcttttg tctcaatact acgggggatc aggttctgtg
     4321 cgaccagtcc cagcgtccag ttgtcatcca ggtccgtact ggcaccgatg tcggcgttga
     4381 tgccattttt cgtgttgtgg tagcggtcgc ctttgaaggc gctgctgtca taatttctga
     4441 ccagcacgtt gtagttgaac aggtcgacgc gctggaattt gggggggaag cccagagacc
     4501 atttctgccc ggccgtttcc agttccgtag ccagagcaat acccacatca gtgatcagtg
     4561 ccgcgcgggc aaaggcctgt gatgtcaggc ggcttttgtc cacctcctgc ccggtgctgt
     4621 ttgccacctc ttccaggtaa gtgaggtcag catcagagac tttaccgtcg acactgacgg
     4681 tgccgtgtga tttaaccatc agtgcggcag agaggcgatc accgggaagg gccgccacag
     4741 cagaaacgcc cagctgtgcg gcggcatgtg tgttacggaa gtcccggagg tgttctttca
     4801 ggcgtgcggc agcctcagga acaccgtggt tactttcgat ggcgcggtcg aaggccttcc
     4861 actcatcact gatccggtcg gcgttgtcgg tgatattatc cggattggag agctgcgcgc
     4921 cgacggacgg aagcaggatg ctgggctcat gcgcgacgca aaatccacga tgtatatatc
     4981 agtactaaaa gcgcgacggc ggaagaagct ctgaaactaa tcggtgaact gtacgccata
     5041 gagcacgata tacgcgggtt accggtgtct gaacgcctgg cggtcaggca aatgcagagt
     5101 agaccgctcc tgacttccct gtataagctg atgcaggaga aagaacacac gttatcgaag
     5161 aaatgtcgtc tgggagatgc gttccggtat atcaggaagc actgggctgc gttgtgcaac
     5221 ttctgtgatg atggcctggc tgaggtagat aataatgcag cagaaagagc gcttcgtgca
     5281 gtctgtctcg ggaagaaaac tttttcgagg atcactcatt ccgtgtctga ctgcttcagg
     5341 gtttaatttt gcttctgctg actgaatgat gattgggcag caagtgcaat atcccacata
     5401 aatccagtta actcacgagc caccgccgta atggctactt gtgatttttt cccttttgct
     5461 gtcagagttc ggtaacgaga acacaatcgt tgttgagctt tccatgcaat atctttggaa
     5521 tattgtgtta cagagtccgg tcggtaatat ataagccatg ctccaacttt tgcaggtgta
     5581 cgataagacc aagcggcttc gtaaagcaga cgtctcagtt cgctgtttcc aacttttgtt
     5641 attcctctgg gacgaatact gtttccactg gaatattcac ctggcacc
//
LOCUS       DAKISA010000181         5640 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.184, whole genome shotgun sequence.
ACCESSION   DAKISA010000181 DAKISA010000000
VERSION     DAKISA010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.184"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="OVW45_004153"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="OVW45_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCV5528458.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="OVW45_004154"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="OVW45_004154"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCV5528459.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILGQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="pgeF"
                     /locus_tag="OVW45_004155"
     CDS             1882..2613
                     /gene="pgeF"
                     /locus_tag="OVW45_004155"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCV5528460.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="OVW45_004156"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="OVW45_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCV5528461.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1400 a         1353 c         1530 g         1357 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agtggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcaccacgt tctgtatagt actcggcaac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgtgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgga taaaagaatg
     1021 gatcctcggc cagcgcgtgc tggttaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc ccgctggata tcgtctatga agatgaagat atcattgtca ttaataaacc
     1201 gcgcgaccta gtagtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattat tatccgccca ttgccgatgt accgcgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc accggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctctggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc actgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgaggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat tgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc ggcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcctggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga cgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgag tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaacattc tgttgcaggt actggatgat ggtcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tttgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggtaagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggggct
     5341 cgtttttgtc tataagttag acggaaaaga ctatatttaa gatgttttgc ctgaaaaatg
     5401 agcgaacgat aaagttttta tatttttcgc ttgtcaggcc ggaataactc cctataatgc
     5461 gccaccactg acacggaaca acggcaaaca cgccgccggg tcggcggggt tctcctgaga
     5521 attccggcag agaaagcaaa aataaatgct tgactctgta gcgggaaagc gtattatgca
     5581 caccccgcgc cgctgagaaa aagcgaagcg gcactgctct ttaacaattt atcagacaat
//
LOCUS       DAKISA010000182         5619 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.185, whole genome shotgun sequence.
ACCESSION   DAKISA010000182 DAKISA010000000
VERSION     DAKISA010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5619
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.185"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..512)
                     /locus_tag="OVW45_004157"
     CDS             complement(<1..512)
                     /locus_tag="OVW45_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCV5528462.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIIDAELFALAGLDA
                     QHLQLLVHSGS"
     gene            complement(758..1210)
                     /locus_tag="OVW45_004158"
     CDS             complement(758..1210)
                     /locus_tag="OVW45_004158"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCV5528463.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVWVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQHVECRGAWFTNFYMQYKPQH"
     gene            complement(1207..2262)
                     /gene="dinB"
                     /locus_tag="OVW45_004159"
     CDS             complement(1207..2262)
                     /gene="dinB"
                     /locus_tag="OVW45_004159"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCV5528464.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(2333..3118)
                     /gene="lafU"
                     /locus_tag="OVW45_004160"
     CDS             complement(2333..3118)
                     /gene="lafU"
                     /locus_tag="OVW45_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCV5528465.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            3090..4802
                     /locus_tag="OVW45_004161"
                     /pseudo
     CDS             3090..4802
                     /locus_tag="OVW45_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(4925..5422)
                     /gene="rayT"
                     /locus_tag="OVW45_004162"
     CDS             complement(4925..5422)
                     /gene="rayT"
                     /locus_tag="OVW45_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCV5528466.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRNAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
     gene            complement(5493..>5619)
                     /locus_tag="OVW45_004163"
     CDS             complement(5493..>5619)
                     /locus_tag="OVW45_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCV5528467.1"
                     /translation="HIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLEGSGLS"
BASE COUNT         1340 a         1467 c         1459 g         1353 t
ORIGIN      
        1 gagccgctat gaaccaacag ttgcagatgt tgtgcatcca gacctgccag tgcaaacagt
       61 tcagcgtcga tgatttgatc aacttgttgc agttcagcga aatgattgcc gccaccgata
      121 gagccaagcg agctgcgcca cggatgctgt gcaaatgctg acggcaggtt ttcctccagc
      181 cagctttctt cagcaacgtc atccaaagct gataatcgct tttcaaactt atcggcgttg
      241 tatttgcgag cgagaatatc tgtttgccat agcgccatgc cgcagccgat atcattgccg
      301 accagtgctg ggtaaaaacg gccaacagag aagaacgctg cgccaatcgg gtagccgcgt
      361 ccgggatgta aatctggcat ccccactacg cgctgcatgt tgggtaaatt tgccgtggtg
      421 tgtaattgtt ggatcgctaa actctcgatc cacaggtcat cagatgcgat ggtaaatacc
      481 gcatcggata agggacgaat atatttgccc atgtaccatt ccgtaaatag actcgtcata
      541 cttcaagttg catgtgctgc gactcgaatt attgagagta acaattaaaa aatcaggaaa
      601 tggcagacga atccagggca agaaaaaaag aattaatcct ggaaaggtct gcaaagagtc
      661 attgataaag gatttatcat gatttacctc cttgcagttt gtggaataag aaaacgcgca
      721 catactaggc gggcgttatt ttcattgcaa gctggattta atgttgcggt ttatattgca
      781 tataaaaatt agtaaaccac gctccccggc attcaacgtg ctgctgctta actgtctgaa
      841 aaccataacg ttcaaaaaag ggttttgcgg ttatgctggc atctaccgta agttcggatt
      901 cagacttaat aaatggtttt aacaaggcgc tggcaacccc gcggcgggtg tattcagggt
      961 caacaaataa catatcgata taatgttcaa tgcgggaaat aaaaccaacc ggttgtgcat
     1021 taatgaccgc aacccacact tgtgatttcg cgagtttctc cttccagcga gattcgtcaa
     1081 tctgcgccca ggcggcaatt tgttgtggag aataatgctg actggcggtc atcgtaaccg
     1141 ctctaatgaa aatagcgcat agttgctgaa aatcgccagg ctgatagttt cttatttgta
     1201 tgttattcat aatcccagca ccagttgtct ttccatttgc ggatcaagca acgtcacatg
     1261 cagccccacc agtcgcacac cgcgcccgcc gcggcgttca tcccaggttt tacgcgcggt
     1321 ggcgattaag tcagctttat tcagccgcgg ccagacgtgc tcctgagtgg tttgctgaaa
     1381 atcgtcgaac tttaatttca ccccctggcg ggcaatcagt aaatcaggtt ttacctttgc
     1441 cagacggcgt tcaagttccg gatacagccg ctcgataatc gcttcacatt cagaccagtg
     1501 gtggatatct tccgccatcg tgcgttccac gccgacggat tttcgcaacc gttcgctgtt
     1561 aacgtcgcgc tcgtcaatcc cctgactacg ctcccacaaa atgcggccaa atttgccaaa
     1621 gcgtttaagc agcatcacca gatcacactt ttgtacatca ccgcaggttc gtagccccat
     1681 cgcttccagt tttgccgccg agactttgcc gacgccgggg atttttgcca gtggtaaggt
     1741 ttgtaaaaat gccggaactt ctgccggcgt aatcacaaac tggccgttgg gtttattcat
     1801 gtcggaggcg attttggcga gaaactttac gggtgccacg cccgcagacg ccgtcagttg
     1861 cagctcgttg aaaatcgtct ggcggatttc ctgggcgatg agggtcgcag aaccgtggca
     1921 atggacgcta tcggtgacgt cgagataagc ctcatccagt gacaacggtt caatacgcga
     1981 ggtgtagcgc gagaagattt cgcggatatg atttgaggct tctttgtagg cgtcaaagcg
     2041 ccccggaagc aaggtgagat gcgggcataa tttgagcgcc atccctgtcg gcatagcgct
     2101 acgtacgcca aatttacgcg cgggataatt ggcggtactg atcacccccc gacgttcgcg
     2161 gctgccgcca atagcaatag ggatatcgcg cagggcggga ttgtcgcgca tctccaccgc
     2221 tgcgaaaaag cagtccatat ccacatgaat gattttacgc attgctcacc tctcaacact
     2281 ggtaaagtat acagtgattt cagagtttga gaaatgcgta aagattcagc atttaacgcg
     2341 ttcgctgcga aagcacctgc tgcttatcca gcttttgcac cagcggctgc accactttat
     2401 ccccatgctg accaaagtat tgatacagcg tatcggacgc acttttggtc agcaccataa
     2461 tctcaatgcg ccggttgccc gcgctttgcg gatttttgga atccagcagc atctggtccg
     2521 ccattgcgct tacctgcatc actttatctt ccggcattcc ggcctcttcc agcacccgac
     2581 gagccgaaag cgcgcgatcg cccgaaaggt tccagttgtt gtagatattg tttttgtaag
     2641 ccatcgcatc ggtatgcccg gtaataataa ttttgttatc gagcgagtcg aaaactggcg
     2701 cgagctccac cagcagcgtt ttaaagaacg gcataatctg cgcgctgccg cgttcgaaca
     2761 tattgcgatt ctggtcgtct ttaatcagca cgcgtaatcc ctggggaaca atctccatct
     2821 ccagattcgc ttccatatgc gcatcacggg caatggtggt gatactggtt gccagctcgc
     2881 caagctcggt ggcggatttc ttcttcagca gcgccgtttt ctcattgacg tcgcgcgctt
     2941 ttttctccgt ttcttccggc accgccacgg tggcaggttt tggcggatga gacgggctga
     3001 ttttatttaa cggcgacagc ccgccaccgt taaaaatcga ctgcccgtgc agcgcggcga
     3061 taatgctttc acgctcggat ttactgacgc tgttgacaat caactttatt gtcgtcacca
     3121 aaggggccga gcgtatctcc gaggtttctg cccgctttac cctggacgcg atgcccggta
     3181 agcagatggc gattgacgcc gatcttaatg ccgggttgat caatcaggcg caggcacaaa
     3241 cccgacgtaa agatgttgcc agcgaggccg atttctacgg cgcgatggac ggggcatcga
     3301 aatttgtgcg cggggatgct atcgccggga tgatgattct ggcgatcaac ctgatcggcg
     3361 gcgtctgtat cgggatcttc aaatacaacc tgagcgccga cgccgccttc cagcagtatg
     3421 tgctgatgac catcggcgac gggctggtgg cgcaaatccc ttccctgcta ctctccaccg
     3481 cggcggcgat tatcgtcacc cgcgtcagcg acaacggcga tatcgcccat gacgtgcgta
     3541 accagttgct ggcaagccca tcggtgctct acaccgctac cggaattatg ttcgtactgg
     3601 cggtagtgcc gggaatgcca catctgccgt ttttgctgtt cagcgccctg cttggcttta
     3661 ccggctggcg gatgagcaaa cgcccgcagg cggcggaagc ggaagagaaa agtctcgaaa
     3721 cgctgacccg caccatcacc gaaaccagcg agcaacaggt cagttgggaa accattccgc
     3781 tgatcgagcc catcagctta agcctcggct acaagctggt ggcgctggtg gacaaagccc
     3841 agggcaaccc gctcacccag cgtattcgcg gcgtgcgaca ggtgatatcc gacggcaacg
     3901 gcgtgctgct gccggagatc cgcattcggg aaaacttccg ccttaagccc agccagtacg
     3961 ctattttcat caacggcatt aaggctgatg aagcggatat tccggcggat aaactgatgg
     4021 cgctgccctc cagcgaaacc tacggcgaga tagacggcgt gctggggaac gacccggcgt
     4081 acgggatgcc ggtgacctgg atccagcctg cgcagaaagc gaaggcgctg aatatggggt
     4141 atcaggtgat cgacagcgcc agcgtgattg ctacccatgt aaataagatt gtgcgcagct
     4201 atattcctga tttatttaac tatgacgata ttacgcagtt gcataaccgt ttgtcgtcga
     4261 tggcaccgcg cctggcggag gatttaagcg cggtgctcaa ttacagccag ttgctgaaag
     4321 tgtaccgggc gctgctgacc gaaggcgttt ccctgcgcga tatcgtcacc atcgccaccg
     4381 tgctggtcgc cagtagcgcg gtgaccaaag atcatattct gctggcagcc gatgtgcgcc
     4441 tggcgctgcg gcgcagcatt acccatccgt tcgttcgcaa gcaggagctg acggtgtata
     4501 ccctgaataa tgagctggaa aatctgctga ctaacctagt gaatcaggcg caacagggcg
     4561 gtaaagtgat gctcgacagc gtgccagtgg acccgaatat gctcaaccag ttccagagca
     4621 cgatgccaca ggtgaaagag cagatgaaag cggcggggaa agacccggtg ctgctggtgc
     4681 caccgcagct gcgccctttg ctggcgcgtt atgcaaggtt gtttgcgccg gggctgcatg
     4741 tgctgtcgta taacgaagtg ccggacgagc tggagttgaa gattatgggg gcgttgatgt
     4801 aagtatccgg cattggtgca caactgccgg atgcggcgta aacgccttat ccggcctacc
     4861 gttcaggcac aagtttgtag gcatgataag acgcgccagc gtcgcatcag gcatctgcgc
     4921 ataattataa aatgatacgc tcgccagcat ttatatccgt tacgtccccc gcccaatcga
     4981 tgggatataa ccctcgcgcg acatcgcgat ggaacgttga gaatggccaa tcactcactt
     5041 gctttaccca accatgcttt actggattta tataaatata atcaacatga tgcctataat
     5101 ctttggtatt gcggatggcg tgctcccaaa aacgtggttg ccagatattt ttcaatccac
     5161 aagcatgggt aaattgcttt ttaatttccc gccagcgaga ggaaaaatca tcatcgcctt
     5221 caggtaatgt ccatatgcaa tgcatatgct ctggcaaaac gacccaggcg ttgatttcaa
     5281 aaggcctgtc tcgcttaact ttaataatgg cgttacggag catctggtac tgggtggtca
     5341 agagttggct tcgacgattg cgtaaattca ccgtgaaaaa ccaggttccc cccttgatgt
     5401 aataacgacg atattcagac atggcgactc ctttttctca acggtaagaa gaaaaggaaa
     5461 ggtcggcaat gaggtgatgg aacagggcaa gattacgaaa gcccgctccc ctcaaggact
     5521 gacgccagat agtttctgtc catggctgct tttcgcatct tacgtcttaa ccctgccttg
     5581 aataccttat cattcgtcaa aatattaata gcgatgtgc
//
LOCUS       DAKISA010000183         5614 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.186, whole genome shotgun sequence.
ACCESSION   DAKISA010000183 DAKISA010000000
VERSION     DAKISA010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.186"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            11..427
                     /gene="soxR"
                     /locus_tag="OVW45_004164"
     CDS             11..427
                     /gene="soxR"
                     /locus_tag="OVW45_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCV5528468.1"
                     /translation="MAKRSGVAVSALHFYESKGLITSIRNSGNQRRYKRDVLRYVAII
                     KIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREELDRRIHTLVALRDELDG
                     CIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            973..2322
                     /gene="ghxP"
                     /locus_tag="OVW45_004165"
                     /gene_synonym="yjcD"
     CDS             973..2322
                     /gene="ghxP"
                     /locus_tag="OVW45_004165"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCV5528469.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWCKLNIGTVVIAVALVTFYAGGWAI"
     gene            2474..4123
                     /locus_tag="OVW45_004166"
     CDS             2474..4123
                     /locus_tag="OVW45_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCV5528470.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(4277..5569)
                     /locus_tag="OVW45_004167"
     CDS             complement(4277..5569)
                     /locus_tag="OVW45_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCV5528471.1"
                     /translation="MRYNGLNNMFFSLCQINDNHSFTSSSHTKKTKSYNYSKHHKNTL
                     IDNKALSLFKMDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKHFIDIALYANNK
                     CDDRITRRVYLSKEKEVSIKVVYFINNVAVHNNTIEIPQTVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSVMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEANLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTDSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
BASE COUNT         1256 a         1269 c         1456 g         1633 t
ORIGIN      
        1 ccccggcgaa gtggcgaaac gcagcggtgt ggcggtatcg gcgctgcatt tctatgaaag
       61 taaagggttg attaccagta tccgtaacag cggcaatcag cggcgatata aacgtgatgt
      121 gttgcgatat gttgcaatta tcaaaattgc tcagcgtatt ggcattccgc tggcgaccat
      181 tggtgaagcg tttggcgtgt tgcccgaagg gcatacgtta agtgcgaaag agtggaaaca
      241 gctttcgtcc caatggcgag aagagttgga tcggcgcatt cataccttag tggcgctgcg
      301 tgacgaactg gacggatgta ttggttgtgg ctgcctttcg cgcagtgatt gcccgttgcg
      361 taacccgggc gaccgcttag gagaagaagg taccggcgca cgcttgctgg aagatgaaca
      421 aaactaaagc gccacaaggg cgctttagtt tgttttccgg tctttgtctt tctctctatc
      481 ccgctggtac acaggagggt ttcccccgac gtcaacacac cccattcgag cacgtggtgg
      541 aggttccggt tggtgttgat gctttaattg tatgtcaccg gcatttcttc gccagtgtaa
      601 aagtatactt tttatccgca atatttttgt catctcagac gattttttat cgcaatcctg
      661 aacggtatac ggctcgataa cgctgcaatc ttgcgcaccg acgataacgt ttgcgcatca
      721 attgcctggt ttttcatcgt caagacaata aaagagaaaa aagcagcaaa cttcggttga
      781 aaaagccgct atgatcgccg gataatcgtt tgcttttttt accacccgtt ttgtatgcgc
      841 ggagctaaac gtttgctttt ttgcgacgca gtaaattgtc gcaaacctgg agcaggaaga
      901 taacgtttcg ctggcagggg attgtccgcc acgcatcttg acgaaaatta aactctcagg
      961 ggatgttttc ttatgtctac gccatcagcg cgtaccggcg gttcactcga cgcctggttt
     1021 aaaatttcac aacgtggaag cactgtccgt caggaagtgg ttgccgggtt aacaacgttt
     1081 ctggcgatgg tctactcggt catcgtcgtt ccaggtatgt tgggtaaagc aggcttcccg
     1141 cctgcggcag ttttcgttgc aacctgtctg gttgccggac tcggttctat cgtgatgggt
     1201 ctgtgggcta atctgccgtt ggcgattggt tgcgccatct ccctgacggc gtttaccgca
     1261 ttcagcctgg tgctggggca acatattagc gtacctgtcg cgctgggtgc cgtgttcctg
     1321 atgggtgtgc tgtttacggt aatttctgcc acgggtatcc gtagctggat tttgcgcaac
     1381 ttgccgcacg gtgtggcgca cggcacgggg atcggtatcg gtctgttcct gctgctcatt
     1441 gccgctaatg gtgtcggtct ggtgattaaa aacccgcttg atggtctgcc cgttgcgctg
     1501 ggtgatttcg cgaccttccc ggtgattatg tcactggtag gtctggcggt gatcatcggc
     1561 ctggaaaaac tgaaagtccc tggtggcatt ctgctgacca ttatcggtat ctcaattgtc
     1621 ggtttgatct tcgatcctaa cgtccatttc tccggcgttt tcgccatgcc ttcattgagc
     1681 gatgaaaacg gcaattcact gattggcagc ctggacatta tgggcgcgct gaatcctgta
     1741 gtcctgccaa gcgttctggc gctggtgatg acggcagtat ttgatgccac cggaactatc
     1801 cgtgccgtcg ccggccaggc gaacctgctg gataaagatg ggcagatcat cgacggtggg
     1861 aaagcactga ccactgactc catgagcagc gttttctctg gcctggtggg tgctgctccg
     1921 gcagcggtat acatcgagtc tgcggcgggt acggcggcgg gcggtaaaac cggtttgacg
     1981 gctatcaccg ttggcgtgct gttcctgctg attctgttcc tctctccgct ctcttacctc
     2041 gttccggggt atgcaacggc tccggcgctg atgtacgttg gcctgctgat gctgagcaac
     2101 gtggcgaaaa tcgactttgc tgattttgtt gatgcgatgg cgggtctggt tacggcggta
     2161 ttcatcgtgc tgacctgtaa catcgtaaca ggcatcatga tcggcttcgc gactctggtg
     2221 attggtcgtc tggtttccgg cgaatggtgc aagttgaaca tcggtacggt cgttatcgcc
     2281 gtggcactgg tgaccttcta tgcgggtggc tgggctatct aatctttcat cggattctga
     2341 aaacgggtgg caatggctgc ccgtttttat tttctccgca cattgtgtga gcttttgcga
     2401 tattctgaaa aaatgagaat tcaggcataa cgcctttctc agatcataaa aaaacatcgc
     2461 aaacagggaa cgcatggaaa tcttcttcac catactgata atgaccctcg tggtctcact
     2521 gtccggggtg gtcactcgtg tcatgccctt tcagatcccg cttccgctta tgcaaatcgc
     2581 catcggtgcg ctactggcgt ggccgacgtt tggtttgcat gtggagtttg atcctgaact
     2641 ctttttagtc ttgtttatcc cgccgttgct gttcgctgat ggctggaaaa cgccgacccg
     2701 tgaatttctc gaacatggtc gagagatttt cggcctcgcg ctggcgctgg tggtggtcac
     2761 cgtggtcggc attggcttcc ttatttactg ggtggtgccg ggcattccgc tgatccccgc
     2821 ctttgcgctg gcggcggtac tttctccgac cgatgctgtg gcgctctccg ggattgttgg
     2881 cgaagggcgc atcccgaaaa aaatcatggg cattttgcag ggcgaagcgt tgatgaatga
     2941 cgcctccggc ctggtgtcgt tgaagtttgc cgtggcagtg gcgatgggga cgatgatctt
     3001 caccgtcggc ggtgcgacgg tcgaatttat gaaagtagcc attggtggta ttcttgccgg
     3061 ttttgtggtg agctggctgt atggtcgctc gctgcgattc ctcagccgct ggggcggtga
     3121 tgaacccgcg acgcagatcg ttctgctgtt cttgctgcca ttcgcttctt atctgattgc
     3181 cgaacatatt ggcgtttccg gcatcctcgc tgcggttgcc gccgggatga ccatcacccg
     3241 ctccggtgtg atgcgccgtg cgccgctggc aatgcgcctg cgcgcaaaca gcacctgggc
     3301 gatgctggaa tttgtcttta acggcatggt gttcctgctg ttaggtctgc agctgccggg
     3361 tattctggag acgtcgctga tggcggcaga aatcgaccct aacgtcgaaa tctggatgct
     3421 gtttaccgat attattctga tatatgcggc gctgatgctg gtccgtttcg gctggctgtg
     3481 gacgatgaaa aagttcagca accgcttcct gaagaagaag ccgatggagt ttggttcgtg
     3541 gaccacacga gaaatcctga tcgcgtcttt cgccggggtg cgtggggcga tcactctggc
     3601 cggtgtgctc tctatcccgc tgctcttgcc ggatggtaac gtcttcccgg cgcgctatga
     3661 gctggtgttc ctggcggctg gcgtcattct cttctcgctg tttgtcggcg tggtgatgtt
     3721 gcctattctg ctacaacaca ttgaagtcgc ggaccattcg caacaattga aagaagaacg
     3781 tattgcgcga gcggcaacgg cagaagtggc gattgtggcg atccagaaaa tggaggagcg
     3841 tctggcggcg gataccgaag agaatatcga taaccagctg cttacggagg tcagttctcg
     3901 cgtcattggt aacctgcgtc gtcgcgccga tggacgtaac gacgttgaaa gttccgtgca
     3961 ggaagagaac cttgagcgtc gcttccgtct ggcggcattg cgttctgaac gtgctgaact
     4021 gtaccacctg cgcgccacgc gggagatcag caacgaaacg ctgcaaaaat tactgcacga
     4081 tctcgatttg cttgaagcgt tgctaattga ggaaaatcag taagccgaaa gcgacgtaac
     4141 caggcccgag gtaatctgaa aaaggtgcaa tcttttgcac ctttaacagg ttagctcggg
     4201 ctaacattca tgattctaaa acaaataagt tatctctctt gtaaagtcaa ttcattggct
     4261 gttcttcgac ttaacctcaa tctgcgtgtt gcataatatc tgcagtaatc ttcatagcga
     4321 ctgcattaat attatcctca gtttgtgaca tgccagtatg agaggtacaa tcttcttcaa
     4381 agaatttctg tagtgttacc agggtatcac tatcaatggg gatattttcc tccccacgga
     4441 gtatggaaat gcccattccc tggacgctag cctcatcaaa attaacccga tagagacggg
     4501 tattaaacat acaggctttt gttaaaatag aacagttgaa atggcagttg cttaaattgg
     4561 cattattaaa attaacatta gccatattag agtcggtaca tataaccatt tcgaggaatg
     4621 tatttgttag ccacgtacaa aagagtttgc tatcaattaa gtttatattc tggaggattg
     4681 ctttgttcag attgacgcga gacagatcag catgttcaaa gttaattaaa tcgagtttag
     4741 agaaactaag gtcggcttta aataaattaa catatggcgc tattacttta taaagatttg
     4801 cctcagccat gtacgccata taaaaatttg aatagtcaag aaaggtattt ttaatattgg
     4861 ctctattcat tgctgcattg tagaattgaa catgcataac ggagcatgca tttagagttc
     4921 cgtcggccat ttttatctga gcgaaatttg agtcatcgag aatacaatta tcaaaaagta
     4981 ttttttccat tatggcgtaa taaaaattcg ttttatacat attacagtcc tggaaaatag
     5041 cgttttgtag tctgcaacct gcaaaattcg aattggataa atcttcatct ttaatcacga
     5101 tacctttcag gctaaggtgt gaaaaatcgt aaccaccatt tactgtctgt ggaatttcga
     5161 tagtattatt atggacggcg acattattta taaaatatac caccttaatg gatacttcct
     5221 tttctttact aaggtaaact cttctcgtaa ttctatcgtc acatttatta tttgcatata
     5281 aagctatatc tataaaatgt ttatgtattt tcctgtccgt ttccttcgtg atttttcctg
     5341 atggtaaact atcataaatt cttttcattt tctgaatcaa acctatcact ttttcatgat
     5401 catccatctt gaaaagagag agggctttat tgtcaattaa cgtgttttta tgatgtttgc
     5461 tgtaattata tgattttgtt ttctttgtat gtgatgaact tgtgaaagag tggttatcgt
     5521 taatctggca aagagagaaa aacatattat ttaaaccatt atagcgcata aaatatcctt
     5581 ataattaaca agaaagaaaa ggcatattct ctgc
//
LOCUS       DAKISA010000184         5568 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.187, whole genome shotgun sequence.
ACCESSION   DAKISA010000184 DAKISA010000000
VERSION     DAKISA010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5568
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.187"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            239..1153
                     /gene="sitA"
                     /locus_tag="OVW45_004168"
     CDS             239..1153
                     /gene="sitA"
                     /locus_tag="OVW45_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCV5528472.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            1153..1980
                     /gene="sitB"
                     /locus_tag="OVW45_004169"
     CDS             1153..1980
                     /gene="sitB"
                     /locus_tag="OVW45_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCV5528473.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNDSEESIITDDERPFVAHRPSAVQREAR"
     gene            1977..2834
                     /gene="sitC"
                     /locus_tag="OVW45_004170"
     CDS             1977..2834
                     /gene="sitC"
                     /locus_tag="OVW45_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCV5528474.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            2831..3688
                     /gene="sitD"
                     /locus_tag="OVW45_004171"
     CDS             2831..3688
                     /gene="sitD"
                     /locus_tag="OVW45_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCV5528475.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(3889..4512)
                     /locus_tag="OVW45_004172"
     CDS             complement(3889..4512)
                     /locus_tag="OVW45_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCV5528476.1"
                     /translation="MLMSLAQCLGFLFAHREECTVEIKKIINPRYTESCAVDCDVFFD
                     DRDQAVPYTATADDVAPTGQQIWQELQSGKWGEIAPFTVTPEMLEAAREARRQGIEAW
                     RTEQEAKPFTFEWNGRIWNAGPNSLGRLYPVVMAAKSDIVRDVMTWGDADNQQVKLSM
                     PELEELAAAMAQAQVERNDEIYRRQRELKEELNSLKDLNSVRNFIVE"
     gene            complement(4463..5551)
                     /locus_tag="OVW45_004173"
     CDS             complement(4463..5551)
                     /locus_tag="OVW45_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000035937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCV5528477.1"
                     /translation="MAQAQQSAGAAAGNARQTAQDVAAAATARDDAQRFAEKARQDAT
                     VTAEDRKATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKMANHL
                     SEIAAAGEKAQQKSRDSLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPEDVI
                     RFDTKSDFLAWVRNALPGEYSVAGPYGIIIPDTRFEGGLSIRWTDARPETTEPRYRAK
                     SLTFYGINGPIYHTRYCYWPISRLTDWVKINITTEDIIYRIVVSSVRNRWGDPDIGGL
                     IIAAYQGEADGDKVIRLVRGQSYRGSRLGPVGISVPSTPTGTYIASPQFFITGCSEHS
                     LPGSYCALSGVPDAHVSGAMLGLFIRTS"
BASE COUNT         1256 a         1441 c         1383 g         1488 t
ORIGIN      
        1 cttctacagc gttttgcccc atttttgcca tcaacaattt tcctcataaa gtcatttcaa
       61 agcctaatag ctgctctact tttttcctca atatcatgtc gccagcagga aacaacctaa
      121 tccctgtttt aggcctcaaa gctgttaatg ataatcattt tcattttgta tgggttatgc
      181 tataaaacat agccgttgct atgtttgttc taattttgct cactataggt actaaattat
      241 gcactcaata aaaaaagtaa ccatgctctt ggggtgcctc gccctcacct gctcgatcgc
      301 atttcaggca agtgcaactg aaaaattcaa ggtcattaca acatttacca tcatcgcgga
      361 tatggccaaa aacgtggctg gagatgctgc agaagtctca tccataacca agcctggtgc
      421 agaaattcat gagtatcagc ctacccctgg cgatattaaa cgtgcgcagg gggcacaact
      481 gattctcgcc aatggtatga atctggaatt gtggttccaa cgcttttacc agcatctcaa
      541 tggggttcca gaagtaattg tctcttcggg tgtgacgcca gtagggatca ccgaaggacc
      601 ctatgagggc aaacctaatc cccatgcatg gatgtcgcca gataatgctc tgatttacgt
      661 cgataatatt cgtgatgcgt tgataaaata cgatccggca aatgcacaaa cctaccaacg
      721 taatgccgat acttataaag ccaagattac ccaaaccctt gcccccctgc gtaagcagat
      781 tacggaactc cctgagaatc agcgatggat ggtcaccagt gaaggggctt tttcttatct
      841 cgcacgcgat ttggggctaa aagagcttta tctgtggccg attaatgccg atcaacaagg
      901 aacaccgcag caggtacgta aggttgttga tatagttaag aaaaatcata tcccggcagt
      961 ctttagcgag agtacgattt ccgataaacc agcgcgtcag gttgcgcgtg aaaccggcgc
     1021 acactacggc ggtgtgctct atgtcgattc cctgagcaca gaaaacggcc cggtacctac
     1081 gtatatcgac cttcttaagg ttactaccag tacgctggta cagggaatta aagccgggaa
     1141 aagggagaaa taatgatgca atctgcaggc attgtcgtta atgatgttac tgtcacctgg
     1201 cgtaacgggc acacagcact gcgcgatgca tccttcacag taccaagtgg atctattgcc
     1261 gctctggttg gggtaaacgg ttccggaaaa tcgacgttgt ttaaagcgat tatgggattt
     1321 gtgcgtctga ccagcggaaa gatatctgtt ctgggtattc ccacgcgaca ggcgctacag
     1381 aaaaacctgg ttgcctacgt ccctcagtca gaagaggttg actggtcatt tcctgtactg
     1441 gtggaagatg tggtgatgat gggccgctat gggcatatgg gtatgttgcg tatagccaaa
     1501 aagagggatc gtcagattgt caccgatgcg ctggaacgtg tcgatatggt ggattttcgc
     1561 catcgtcaaa tcggcgagct ttccggtgga caaaagaaac gcgtctttct ggcgagagcg
     1621 attgcacagc agggtgatgt gatcctgctg gatgaacctt ttaccggcgt tgatgtgaaa
     1681 accgaagcga aaatcatcag cctgttgagg gagttacgcg cggaaggcaa aacgatgctt
     1741 gtctcaacgc acaatctcgg ctcagtcacg acattttgcg attatacggt catggtcaaa
     1801 ggcaccgtcc tggcaagcgg gccgacagac accactttta cagccgagaa cctggagctg
     1861 gcttttagcg gcgtactgcg ccatgtcaca cttaacgact cggaagaaag cattattact
     1921 gacgatgaac gcccttttgt ggcacatcga ccgtcagcgg tgcagaggga agcaagatga
     1981 acgtgctact ggaacccttc agctatgaat atatgctcaa tgcgatgtgg gtctcggcaa
     2041 tggtcggcgg tctctgcgct ttcctgtcat gctatctgat gctaaaaggc tggtcgctga
     2101 ttggcgatgc gctgtcgcac tctattgtcc cgggtgttgc gggggcatat atgctggggc
     2161 ttccgttttc tctgggagcc ttcttttccg gcggactggc agcgggcagc atgctgtttc
     2221 ttaaccaacg cacccgctta aaagaagatg ccatcatcgg cctgatcttc tcgtcctttt
     2281 ttggcctggg gctgtttatg gtgtcattga acccgacatc cgtgaacatt cagaccatcg
     2341 tcctgggcaa tattctggca atcgatccgg cggatatcct gcaactcact atcattggca
     2401 ttctctcgat catcgtgctt tttttcaaat ggaaagatct tatggtgacc tttttcgacg
     2461 agaatcacgc ccgtgctatt ggattacatc cgggcagatt aaaaatcctc ttcttcaccc
     2521 tgctgtcggt ttcgaccgtt gcggcgctgc aaacggttgg tgcctttctg gtgatctgtc
     2581 tggtggtgac tcccggtgca acagcatggt tgttaaccga tcgttttccc cgcctgctca
     2641 taattgctgt aactattggc agtgtaacca gtttcctcgg cgcgtgggtc agttactttc
     2701 tggacggcgc gacaggtgga atcatcgtgg tggcgcagac actgctgttt cttttagcat
     2761 ttgtatttgc gcccacccat ggacttttgg caaatcgtcg gcgtgcgcat aaagcgctgg
     2821 aggatcgctc atgatggcat tattactgga gccactgcag ttcaccttta tgagtcacgc
     2881 actgctgatt tcactggtag tttccatacc ctgtgcgctg ctgtcggtct ttctggtcct
     2941 gaaaggttgg gcactgatgg gtgacgcgat gagtcatgcg gtttttcctg gcatcgtgct
     3001 tacctggatt ctggggttac ctctggctac aggggcattc gtcgccggag tattctgcgc
     3061 ggtggcgacg ggatacctga aagacaacag ccgaatcaag caagatacag ttatggggat
     3121 tgttttttct ggcatgtttg ccgcaggtct gatcttgtat attgcaatca aaccagacgt
     3181 acatcttgac catattcttt tcggcgacat gctcgggata accatcggcg atataatcca
     3241 gacggtgatt attgccgggc tggttacact tgttattagc gtaaaatggc gggatttttt
     3301 gctgttcagc tttgattatc aacaggcgca ggtaagcggt ttgcatacga gatggctgca
     3361 ttatgggctg ctgtgcatgg tttccctgac cattgtggcg acgctgaaag cagtaggtat
     3421 cattctctct atttctctgc tcattgcgcc tggcgccatt gcagtactcc tgacacaacg
     3481 ctttcatatt gcgctcctac tggcgactgg catatcggta atagtgtcaa tgactggtgt
     3541 ctggctgtcc ttttttatcg acagcgcacc agctccgacg attgtcgtct tattcgcggt
     3601 cgtgttcatc atgacgttta ccgttaccag catcaacgca cgcacaaagg gaaacgccca
     3661 tacacaggat ctgttatcac ccaattagca caacatccga ggacagcatc gtccgccagc
     3721 ctacagaagt ccggaaagcc atggaaggtc gttgggagga ggtacgaatt ctatcatgca
     3781 aaaaatacgt aaaatcgata acgcctagaa atcattcaat actcgcacta tcgaaagttt
     3841 accagccaac cgcggcacgt tcctgcatac gacgtgccgc agcttctgtt attccacgat
     3901 aaaattccta accgaattca aatccttcag actattcagc tcttccttca gctcccgctg
     3961 acggcgataa atctcgtcat tgcgctcaac ctgtgcctga gccattgctg ccgccagttc
     4021 ttccagttcc ggcatcgaca gtttcacctg ctgattatcg gcatcacccc acgtcatcac
     4081 gtcccgtaca atgtcggatt tcgcagccat taccaccgga taaaggcggc ccagtgagtt
     4141 ggggccagca ttccagatac gaccgttcca ttcaaacgtg aacggcttcg cctcctgttc
     4201 tgtgcgccat gcttcaattc cctgacgtct ggcctctctg gccgcttcca gcatttctgg
     4261 tgtcacagtg aatggggcta tctcacccca tttgccactt tgcagttcct gccagatttg
     4321 ctgacccgtc ggtgcgacat catcagcggt ggctgtgtag gggactgcct ggtccctgtc
     4381 gtcaaaaaaa acgtcacagt ctactgcgca actttcggta taacggggat taatgatttt
     4441 tttaatttcc acggtgcatt cctcacgatg tgcgaataaa aagcccaagc attgcgccag
     4501 agacatgagc atccggcacc ccggacaggg cgcaatatga ccccggtaat gaatgctctg
     4561 aacatcccgt aatgaaaaat tgtggggatg ctatatacgt tccggtggga gtactgggca
     4621 ctgaaatccc caccggtccc agtcgtgagc ctctgtatga ctgccccctg acaagtctga
     4681 tgactttatc accgtcagct tctccctggt acgcagcaat aatcagcccg ccaatgtcag
     4741 ggtctcccca tctgttgcgg acagagctca ccacgattct gtaaataata tcttctgtgg
     4801 ttatatttat tttcacccag tcagtcagtc tggatatggg ccagtagcag tagcgggtgt
     4861 gataaatggg gccgttaatg ccgtaaaaag taagggattt ggctctgtac cgcggttctg
     4921 ttgtctcagg gcgtgcatca gtccaccgga tgctgagccc cccttcaaac cgtgtgtcgg
     4981 gtatgatgat gccgtagggg ccagcaacgg aatattcacc tggcagcgca ttccttaccc
     5041 aggccaggaa atcactctta gtgtcaaaac ggataacatc ttcaggcaga aaagcacacc
     5101 caaagccgaa tgcgccgggt atcgccagac ggccttttgt ccggtcgtaa atgtcgctct
     5161 gtgcttccat cgtggctgca cttttcagcc ccagactatc ccgggacttc tgttgtgcct
     5221 tttcgcctgc tgctgcgatt tctgacagat ggttagccat tttcagagtg ccggtcagcg
     5281 cagcatcaat gtcatttttg gcctgttctg ctgcgcgggc ataacctgcg gcatcctgtg
     5341 cgctctgtcc ggctgcggct gcatttgctc ctgtgcttgt cacatcttcc gcagtggcct
     5401 ttctgtcctc agctgtgacg gttgcatcct gtcgggcttt ctccgcaaaa cgttgtgcat
     5461 catcacgggc cgttgcggct gccgccacat cctgcgccgt ctgccgtgcg tttccggctg
     5521 cagccccggc actctgctgc gcctgcgcca ccatttcctc aaagcgct
//
LOCUS       DAKISA010000185         5532 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.188, whole genome shotgun sequence.
ACCESSION   DAKISA010000185 DAKISA010000000
VERSION     DAKISA010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.188"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            241..582
                     /gene="raiA"
                     /locus_tag="OVW45_004174"
     CDS             241..582
                     /gene="raiA"
                     /locus_tag="OVW45_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCV5528478.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    685..810
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            686..733
                     /gene="pheL"
                     /locus_tag="OVW45_004175"
     CDS             686..733
                     /gene="pheL"
                     /locus_tag="OVW45_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HCV5528479.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            833..1993
                     /gene="pheA"
                     /locus_tag="OVW45_004176"
     CDS             833..1993
                     /gene="pheA"
                     /locus_tag="OVW45_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCV5528480.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINMSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="OVW45_004177"
     CDS             complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="OVW45_004177"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCV5528481.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="OVW45_004178"
     CDS             complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="OVW45_004178"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCV5528482.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4447..4812
                     /locus_tag="OVW45_004179"
     CDS             4447..4812
                     /locus_tag="OVW45_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCV5528483.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVESASQ
                     LHEVWQKGADENASTIRLN"
     gene            4962..5480
                     /locus_tag="OVW45_004180"
     CDS             4962..5480
                     /locus_tag="OVW45_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCV5528484.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
BASE COUNT         1521 a         1393 c         1241 g         1377 t
ORIGIN      
        1 tgagctgccg tttttttatt ctgttagttg tgaaactgaa gcgatttagt cactatcgat
       61 ctcatcaaat atggctcgct ttgagatatt cctcaagtaa aaaaacacct cttcctgcga
      121 tttctcacaa aaaagattcg ttgacaaaaa gtgacaaaat tatgagattc ccatcacaca
      181 ttttgacatc aggaacggta tgctgaattc accaagacgg gaagacaaga ggtaaaattt
      241 atgacaatga acattaccag caaacaaatg gaaattactc cggccatccg ccaacatgtc
      301 gcagaccgtc tcgccaaact ggaaaaatgg caaacacatc tgattaatcc acatatcatt
      361 ctgtccaaag agccacaagg gtttgttgct gacgccacaa tcaatacacc taacggcgtt
      421 ctggttgcca gtggtaaaca tgaagatatg tacactgcaa ttaacgaatt gatcaacaag
      481 ctggaacggc agctcaataa actgcagcac aaaggcgaag cacgtcgtgc cgcaacatcg
      541 gtgaaagacg ccaacttcgt cgaagaagtt gaagaagagt agtcctttat attgagtgga
      601 tcgccaacgc gccttcgggc gcgttttttg ttgacagcgt gaaaacagta cgggtactgt
      661 actaaagtca cttaaggaaa caaacatgaa acacacaccg tttttcttcg cattcttttt
      721 taccttcccc tgaatgggag gcgtttcgtc gtgtgaaaca gaatgcgaag acgaacaata
      781 aggcctccca aaccgggggg ccttttttat tgataacaaa aaaggcaaca ctatgacatc
      841 ggaaaatcca ttactggcgc tgcgagagaa aatcagcgcg ctggatgaaa aattattagc
      901 attactggca gagcggcgcg aactggccgt cgaggtggga aaagccaaac tgctctcgca
      961 tcgcccggta cgagatattg atcgtgaacg cgatttactg gaaagattaa ttacgctcgg
     1021 taaagcgcac catctggacg cccattacat tactcgcctg ttccagctca tcattgaaga
     1081 ttccgtatta actcagcagg ctttgctcca gcaacatctc aataaaatta atccgcactc
     1141 agcacgcatc gcttttctcg gccccaaagg ttcttattcc catcttgcgg cgcgccagta
     1201 tgctgcccgt cactttgagc aattcattga aagtggctgc gccaaatttg ccgatatttt
     1261 taatcaggtg gaaaccggcc aggccgacta tgccgtcgta ccgattgaaa ataccagctc
     1321 cggtgccata aacgacgttt acgatctgct gcaacatacc agcttgtcga ttgttggcga
     1381 gatgacgtta actatcgacc attgtttgtt ggtctccggc acgactgatt tatccaccat
     1441 taatacggtc tacagccatc cgcagccatt ccagcaatgc agcaaattcc ttaatcgtta
     1501 tcctcactgg aagattgaat ataccgaaag tacgtctgcg gcaatggaaa aggttgcaca
     1561 ggcaaaatca ccgcatgttg ctgcgttggg aagcgaagct ggcggcactt tgtacggttt
     1621 gcaggtactg gagcgtattg aagcgaatca gcaacaaaac ttcacccgat ttgtggtgtt
     1681 ggcacgtaaa gccattaaca tgtctgacca ggttccggcg aaaacgacgt tgttaatggc
     1741 gaccgggcaa caagccggtg cgctggttga agcgttgctg gtgctgcgca accacaatct
     1801 gattatgacc cgtctggaat cacgcccgat tcacggtaat ccatgggaag agatgtttta
     1861 tctggatatt caggccaatc ttgaatcagc ggaaatgcaa aaagcattga aagagttagg
     1921 ggaaattacc cgttcaatga aggtattggg ctgttaccca agtgagaacg tagtgcctgt
     1981 tgatccaacc tgatgaaaag gtgccggatg atgtgaatca tccggcactg gattattact
     2041 ggcggttgtc attcgcctga cgcaataaca cgcggctttc actctgaaaa cgctgtgcgt
     2101 aatcgccgaa ccagtgctcc accttgcgga aactgtcaat aaacgcctgc ttatcgccct
     2161 gctccagcaa ctcaatcgcc tcgccgaaac gcttatagta acgtttgatt aacgccagat
     2221 tacgctctga cgacataata atgtcggcat aaagctgcgg atcctgagca aacagtcgcc
     2281 cgaccatcgc cagctcaagg cggtaaatcg gcgaagagag cgccagaagt tgctcaagct
     2341 gaacattttc ttctgccaga tgcagcccat aagcaaaagt agcaaagtgg cgcagagcct
     2401 gaataaacgc catattctga tcgtgctcga cagcgctaat acgatgcagc cgagcgcccc
     2461 agacctgaat ttgctccaga aaccattggt atgcttccgg cttacgtcca tcacaccaga
     2521 ccacaacttg ctttgccagg ctaccgctgt ccgggccgaa catcgggtgt aaccccagta
     2581 ccgggccatc gtgcgccgcc agcatggcct gtaatggtcc atttttcact gatgccagat
     2641 caaccagaat acaatctttc ggtaaaggcg gtaatttgcc gataacttgc tcagtaacgt
     2701 ggattggcac actaacaatc accattccgg catcggcaac aatatcagcc gctcgatccc
     2761 agtcatgttg ctccagaatc cgcacctgat aacccgagag tgtcagcatc ttctcgaaca
     2821 ggcgtcccat ctgaccgcca ccgccgacga taaccaccgg acgcagtgac ggacaaagtg
     2881 ttttaaatcc tttgtcgttt tcactggagt aagattcacg catcacccga cgcaaaacat
     2941 cctcaatcag atctggcggt acacccagag cttccgcctc tgcacgacgc gaggccaaca
     3001 tagatgcctc gcgctccgga acataaatag gcagtccaaa gcggcttttc acctcgccca
     3061 cttcagcaac cagttccaga cgcttcgcta ataaattcag cagcgcttta tcgacttcat
     3121 caatttgatc gcgtaatgcg gtcaattcag caaccataat aaacctctta agccacgcga
     3181 gccgtcagct gcccgttcag atcctgatga atttcacgca gcaaggcatc ggtcatttcc
     3241 cagctaatgc aggcatcggt tacggataca ccgtatttca tttcactgcg cggttgctca
     3301 gaagactgat tgccctcgtg gatattactt tcgatcatca gaccaataat tgagcgattg
     3361 ccatctttga tttgagcaac cacggattct gccaccgcag gctgacggcg ataatcttta
     3421 ttggaattac cgtggctgca atctaccatc agagacgggc gcagtcccgc ctgttccatc
     3481 tctttttcac attgcgcaac atccgcaggg ctatagttcg gcgctttacc accgcgcagg
     3541 atgacatgcc cgtccggatt cccctgagtt tgtagcaacg caacctgccc tgcctggtta
     3601 atgccaacaa aacggtgcgg ctgggcggcg gcgcgcatag cgttaattgc tgttgccaga
     3661 ctgccgtcgg tgccgttttt aaaaccaacc ggcatggaaa gcccggaggc catttcacgg
     3721 tgagtttgcg attccgttgt acgagcacca attgctgacc agctaaacag atcgcccagg
     3781 tattgcgggc tattcggatc taacgcttcc gtcgccagtg gcagtcccat attcaccagc
     3841 tcaagcagca atttacgcgc gatctgcagc ccggcttcta catcaaaaga gccatccata
     3901 tggggatcgt taattaaccc tttccagccg acagtggtac ggggtttttc aaaatagacg
     3961 cgcattacca gatagaggct atcgctgacc tctgcggcaa gggctttaaa tcgacgagca
     4021 tattccagag cagtttccgg atcatgaatg gaacaaggac cacaaactac cagcagacga
     4081 ggatcgcgcc cggcgataat atctgaaatg gttttacgcg agtcagcaat ctgggcttct
     4141 tgttgcaggc tcaatggaaa agcggccttc agttgttccg gagtcattaa aacctgttcg
     4201 tcggtaatat gtacgttatt cagcgcgtct ttttgcatga tggcgatcct gtttatgctc
     4261 gtttgcgata gttgatcctc agcgaggatg acgtaacgat aacacataaa gtaaagtttt
     4321 caatccatat ttcgtacatt tttctttaca cgaacaattt gctcgcgctt ttgatgctta
     4381 tttcgtacaa ataaatttac acctccttta aaaacaatcc gctatgcttt gaaaaaggag
     4441 aaagaaatga tgaaaaagtt tatcgccccc ttattggctt tactggttag cggatgtcag
     4501 attgatcctt atactcacgc gccaaccttg accagcaccg actggtatga tgtcggtatg
     4561 gaagatgcga tatcgggcag cgccataaaa gatgacgatg catttagcga ttcacaggcg
     4621 gatcgcggtc tataccttaa aggatatgcc gaaggacaaa agaaaacttg ccagaccgat
     4681 tttacttatg cccggggact ttccggtaaa agctttcctg ccagctgtaa caatgttgaa
     4741 agtgccagcc aactgcatga agtatggcaa aaaggggctg atgaaaatgc cagcacaata
     4801 cgattaaatt aattaaaacc ccacaaataa attaaacata agattttact cattgtgttt
     4861 tatttctcac attgatgacg atcgcatgaa aaaatgatat tttaatgagc attctgaaaa
     4921 aatatcgatg catttcgagc gaagatggtg gggatccctg aatgcgtttt tctcaccgac
     4981 tgttcttact tctgattctc ctgctgacgg gagcaccaat actcgctcag gagccttctg
     5041 acgttgcgaa aaacgtgcgt atgatggttt ccggtattgt cagttacacc cgctggcctg
     5101 cgctatcagg gccaccaaag ttatgtatct tttcatcttc acgttttagt accgccctac
     5161 aagagaatgc cgcgacatct ttgccttatc tgcctgtcat cattcacact caacaagagg
     5221 cgatgatttc aggctgcaat ggtttttatt ttggcaacga gtcaccgaca ttccagatgg
     5281 aattaaccga acaatatccg tcaaaggcgt tattattaat tgccgaacaa aataccgagt
     5341 gcattattgg tagcgccttt tgtctgatca tccacaataa tgacgtcaga tttgccgtaa
     5401 acctggatgc cttatcgcgt agcggtgtaa aggtcaaccc ggatgtctta atgctcgcac
     5461 ggaagaaaaa tgatggataa cgataattct cttaataagc gccccacgtt taaaagagca
     5521 ttacgcaaca tc
//
LOCUS       DAKISA010000186         5250 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.189, whole genome shotgun sequence.
ACCESSION   DAKISA010000186 DAKISA010000000
VERSION     DAKISA010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5250
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.189"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            24..227
                     /locus_tag="OVW45_004181"
     CDS             24..227
                     /locus_tag="OVW45_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528485.1"
                     /translation="MSDINFGIFFIIAFGFITVGSFFIVLIRFLERAETILIGHLDLK
                     SKAIIHGLIILFIYWTLKFFAES"
     gene            339..884
                     /locus_tag="OVW45_004182"
     CDS             339..884
                     /locus_tag="OVW45_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA distortion polypeptide 1"
                     /protein_id="HCV5528486.1"
                     /translation="MKQIKFRLDDAKHHDLLICLKSLYPDEPGLTVSKGMKLLANALL
                     KSKKTNQIQNDYADENNFIKTTLYLTGKQRADIEKAANRHGWTLSRECRYRIQTTLNN
                     RLDFFDQELLMMNRCRNTIDKIGRNFHYIIVNDQTRVLDKDGFYQDAERLKTEIFSLK
                     NQFENYIMLCKGRTVSNKVDV"
     gene            2019..2723
                     /locus_tag="OVW45_004183"
     CDS             2019..2723
                     /locus_tag="OVW45_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCV5528487.1"
                     /translation="MTMTVSLRGKNVVMSNAISRAAQSLSLAEKRIIFAAIAQMGGKY
                     APVRITAQEYANTFGMPLKQAYSQLKEASENFFNRYIRFKLNEVSEIDVDVWKIRWLG
                     AYKYNDGEANVLLHFTPQVMPYLCELEDNFTKYQLSQACALRSVHSWRLLELFEQMNT
                     NKENNGWLSISLEDFHHAMESPDSYKTTFGLLRKYMIEPAVKELTEKDHWMIDWKPIK
                     KGRKVVRLEFWFRKEN"
     gene            3145..3903
                     /locus_tag="OVW45_004184"
     CDS             3145..3903
                     /locus_tag="OVW45_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HCV5528488.1"
                     /translation="MKKRIISAVLASLSVISVAQAGIPVAIDANPEWAVEAGRWTERL
                     KQWAETVKHYENQINAYKQELLSKTGIRDVQGLVQSAQSVSQELMQIYDQGNAFIDDY
                     IKNPESALSEQAKSLLSDYRVTDTCQNLGYSGDQVRGCEATFLSQLASVEYGNKLESK
                     LRQDNQTMKDLIEQVKNAKDTKATQDATNAVALEQLKFEKLKFQYQMYIDKQKQLSEY
                     KKEMAKASFRHEQINAKPIDYREAFSTQDFDLIK"
     gene            3913..4182
                     /locus_tag="OVW45_004185"
     CDS             3913..4182
                     /locus_tag="OVW45_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="HCV5528489.1"
                     /translation="MKYIVLVFLASLSFILTGCNEDTKSVDWWTNHPKEAVDKYKECK
                     KTGSDSDNCKNVRRAGLIIADTYPPMSEIYKQEARELDKKLGVSK"
     gene            4196..>5250
                     /locus_tag="OVW45_004186"
     CDS             4196..>5250
                     /locus_tag="OVW45_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000235774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HCV5528490.1"
                     /translation="MATDNTYPVYDGNFFRATHDIILRAVEKSTSGMFDNYINIAYTL
                     GKYGVSLYILWYAFTVLARKQQTPVPDFIWNLCRFSIILMFVKNTGGWLDNANQAIDG
                     LKETLAGGDPWLWMDQLWDKVITVGKKVWHLDTDTIPVVGVAAALLTYAGGILAMIVC
                     AIVFFSAEITLKILSITAPLFIMCLMFGFLRQMFNNWLQLIFSSLLIFMFAALALRAG
                     MWQLNGTLSSTLSSATAPNLFEAGATVLVSGIFMAWIIWQAKTYASQLAGVGVEGAMQ
                     GAAAMGIGAGIFGASRMTRGALGMGRNAGIGAWKGLRRQEGGFGQSPGITGKAANLAG
                     QGVNIGAKKLRQAAIERA"
BASE COUNT         1579 a          914 c         1178 g         1579 t
ORIGIN      
        1 aaaaaaaatg tacggtggtt aatatgagtg atattaactt tggtatcttt tttataatcg
       61 catttggctt cattactgtt ggttcatttt ttattgtgct tatccgtttt cttgaacgtg
      121 cagaaaccat attgattggt catttagatt taaaatctaa agccattatt catgggttaa
      181 ttatattatt tatatactgg acgcttaaat tttttgcaga atcttaatca aagaaccgac
      241 tgtaggtcac cgggcaaacg gccaaaatgg aaccattttg cggcgtttgc cctatcctgc
      301 atcggagtaa atattcatgt gctgtaggaa gtgcaaatat gaaacaaatc aaattcaggc
      361 ttgatgatgc caagcatcat gacctgctga tctgcctaaa gtctctttat cctgatgaac
      421 caggcttgac tgtatcaaaa ggtatgaaac tgcttgccaa tgcattgctg aaaagcaaaa
      481 aaactaacca gatacaaaat gattatgctg atgaaaacaa tttcatcaaa accacgcttt
      541 acttaacagg taaacaaagg gctgatattg aaaaagcagc taaccgtcat gggtggacat
      601 tatcacggga atgtcgatat aggattcaaa ccacactgaa taataggctt gatttcttcg
      661 accaggaact gctgatgatg aatcgctgcc gaaatactat agataaaatt ggtcgtaact
      721 ttcattatat cattgttaat gatcagacca gggttcttga taaagatggt ttctatcagg
      781 atgcggaacg tcttaagaca gaaattttta gtcttaaaaa tcagtttgag aattacatta
      841 tgttatgtaa ggggagaaca gtttcaaaca aggtagatgt atgaagtcca cattgataac
      901 taaatctagt ttgtattgtt gaataaacta catgttgctg atagagtctt tgcgagatgt
      961 gaatttatcc ttttgttttt ttctttgctt cggggtcatc cgagtcgaag gagcgtctag
     1021 tagaaacgga actactggca aatttgttac tatttcagtc ttgatatagc tgtaaactgt
     1081 ttatagtata aactttgcgc aataataaaa aatctctctc catagagatg tgatttatta
     1141 cgagcaacta cggttagctt gtgttttgtc acgtctaaac gttagtcagt tccagtaatg
     1201 agatcatcgt tgttattcct gtgcaaacgg cctttgcagg gagctggcgc ggtctcctgc
     1261 ttcctgaaag cccctggtta ggggtatcgg cgcgcctgac ctcctgaaac gctctgtaat
     1321 cgtttttcgc gccttccatg ccctgacggg catttaaaac ctgtttcgtg cgtctgagag
     1381 cttctgagcg cgttttaagg gccaatacga agaaaaccat taccaccacc agaaaatcgc
     1441 gttctggtgc tctggatggc tcacagaacg cattttcgaa cagaacagca acggaatcgc
     1501 cgacaacatg cgagaggtgg taccgctcgc cgcagtcgaa cggcaggagc gtagcgactg
     1561 agtgagcgag gaagcggaag tgagtctgta ggtgagttga gcacatacgc atagcggtta
     1621 aaaaacgagc ggattgtttt taggtattgt agctctagct taacgtgatc tgtcctcttg
     1681 gtttgagcat ggttatccac agattttggg ggcaaattcc tttttaaacc ctttttatct
     1741 tttcttttta aaaccttttt agacgccaaa actcttttga aatcatgatg ttatagagtt
     1801 aaaagtaagt tttttcgggt taaaagtaag ttttttcggg ttaaaagtaa gttttttcgg
     1861 gttaaaagta agttttttcg ggcattatcc acagctaact gtaagagtaa aaaatgattt
     1921 tacttttcat tttttgtaag tatgatttac gtacttactc ttgccctgta catcttactt
     1981 ttgagtgaat aatgaccaag aagaatgttg accaaccaat gacgatgact gtcagtttac
     2041 gtggcaaaaa tgttgtcatg agtaatgcta tatctcgtgc cgcgcaaagc ctttctttgg
     2101 ctgaaaagcg catcattttt gcagctattg ctcagatggg tgggaaatat gcgccagtaa
     2161 ggattacagc acaggaatat gctaacacct ttggaatgcc tttaaagcaa gcatattcac
     2221 aattaaaaga ggcttcggaa aatttcttta atagatatat tcgctttaaa ttaaatgaag
     2281 ttagtgagat tgatgttgat gtatggaaga ttagatggtt aggggcgtat aaatataatg
     2341 atggtgaagc taatgttctt ttacatttta cgcctcaagt aatgccatat ttatgcgaac
     2401 tggaagataa ttttacgaag tatcagctat ctcaagcttg cgcattgcgt tcagtacatt
     2461 catggcgttt gttagaactc tttgaacaaa tgaatacgaa caaggaaaat aatggatggt
     2521 tatccatatc gttagaagat tttcatcatg ctatggaatc acccgatagt tataaaacaa
     2581 cgtttggatt acttcgtaag tatatgattg aaccggcggt aaaggagtta acagagaaag
     2641 atcactggat gatcgactgg aaacctatta agaaggggag aaaagtcgtt cgtcttgagt
     2701 tctggtttag gaaagaaaac taatcatatc aatgattaac gctggtaatg agggctgcca
     2761 ccctcactac cagctaacca caacaacctg ttcggaggct gtatggctgg cccaagtata
     2821 tctgaatatc agttaaaaac ccgcaaagaa ttttacgctt atcttgatag taagaggaaa
     2881 gaaggaaact cgagatttag tttatccctt gagaaagacc ctttctcttt ttctttatat
     2941 cttttgttca caagtaatga aggtattcgt aatagtgatt taatccgtcg cttccgaatt
     3001 actagcaggg gcacgaggtt tattgtaagt aactttaaat tctggcttaa ggaatacaat
     3061 cgcggtatgt ttaagtgatg ttagccctct ctttaattaa aggagagggc taattatcag
     3121 gattatattt agtggagatc aataatgaag aaacgcatta tatcggcggt gctcgcttcg
     3181 ctctcggtta tttccgtcgc tcaggcgggg atacctgttg ccattgatgc caatccggaa
     3241 tgggcggttg aagccggacg atggacagaa cgtctgaagc aatgggcaga aacggtgaaa
     3301 cattacgaaa atcagataaa tgcgtacaaa caggagctgc tgtcaaaaac gggtatccgt
     3361 gatgtgcagg gactggtgca gtccgcacag tcagtgagtc aggaactgat gcagatttat
     3421 gatcagggga atgcttttat tgacgattac attaaaaatc ctgaaagtgc gttatcggaa
     3481 caggccaaat cgttattgtc agattacagg gtaacggata cctgccagaa cctgggatat
     3541 tctggtgacc aggtacgggg atgtgaagcg acgttccttt ctcaactggc aagcgtggaa
     3601 tacggtaaca agctggagag caagcttcgt caggacaatc agacaatgaa agaccttatc
     3661 gagcaggtta aaaatgcgaa ggatacgaaa gccacgcagg atgcaacaaa cgccgttgca
     3721 cttgaacaac tgaagtttga gaagctcaaa tttcagtatc aaatgtacat cgataaacaa
     3781 aaacaactgt ctgaatataa aaaggaaatg gcgaaagctt cttttcgtca tgaacaaatt
     3841 aatgcaaaac caattgatta cagagaagca ttttcaacac aagactttga tttgatcaaa
     3901 taaggggttt ttatgaaata tatcgtcttg gtatttttag catctttatc atttattctt
     3961 accggatgta atgaagatac aaaatctgtc gattggtgga ctaatcaccc gaaagaagct
     4021 gtcgataaat ataaagaatg caagaaaaca ggaagtgact cagataattg taaaaacgta
     4081 agacgtgcgg gacttataat cgctgacact tatcctccga tgagtgaaat atacaagcaa
     4141 gaagccagag aacttgataa gaaattaggc gtttcaaaat aactgagggg gttatatggc
     4201 tacagataat acttatcccg tatatgatgg aaatttcttt agagcaactc atgatataat
     4261 attaagagca gttgagaaat caacatcagg aatgttcgat aattatatca atattgctta
     4321 tacactgggt aaatatggtg tttctttata tatcttatgg tatgcattca ctgtcttagc
     4381 aagaaaacaa caaactcctg ttccagattt tatctggaat ttgtgcaggt tttcgataat
     4441 tttaatgttc gttaaaaata cagggggatg gcttgataac gccaatcaag ctatagatgg
     4501 cttaaaagag actctcgccg gtggtgatcc ttggttatgg atggatcaac tttgggataa
     4561 agtaattact gttggtaaaa aggtttggca tcttgataca gatacaatcc ccgttgtagg
     4621 tgttgcagcg gccttgctta cttatgccgg aggcatttta gcaatgatcg tgtgtgcaat
     4681 agtatttttt tcagcagaaa ttacgttaaa aatcctttct attacagcac cgttatttat
     4741 tatgtgtctg atgttcggtt ttcttcgtca gatgtttaat aactggttac agcttatttt
     4801 cagttcatta cttattttta tgtttgcagc actggcgtta cgtgcaggta tgtggcaatt
     4861 gaatggaaca ttaagcagca ctttatcttc agcaacagct cctaatttat ttgaagcagg
     4921 tgcgacagtt cttgtctcag gtatctttat ggcatggatt atctggcagg caaaaaccta
     4981 tgcttcacag cttgctggcg tgggtgttga aggtgccatg cagggcgcag ccgctatggg
     5041 gattggtgct ggcattttcg gtgcatcccg tatgacgcgt ggtgcacttg gtatgggcag
     5101 aaatgccggt attggtgcat ggaaaggtct gcgtcgtcag gaaggcgggt tcggacagtc
     5161 tccgggaata acgggcaaag ccgctaacct ggccggacag ggcgttaata ttggtgccaa
     5221 aaaacttcgt caggccgcta ttgagagagc
//
LOCUS       DAKISA010000187         5163 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.190, whole genome shotgun sequence.
ACCESSION   DAKISA010000187 DAKISA010000000
VERSION     DAKISA010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.190"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            1129..2043
                     /locus_tag="OVW45_004187"
     CDS             1129..2043
                     /locus_tag="OVW45_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCV5528491.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASV
                     KALKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLS
                     ASERRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPV
                     PLSLAECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVAT
                     SARTLKPWEALGISRAWYYQLKKRGLVE"
     gene            complement(2247..2645)
                     /locus_tag="OVW45_004188"
     CDS             complement(2247..2645)
                     /locus_tag="OVW45_004188"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528492.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQER
                     KLLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTK
                     TGELCYKKDRILQALKELKYDLETSPNPDA"
     gene            2822..3748
                     /locus_tag="OVW45_004189"
     CDS             2822..3748
                     /locus_tag="OVW45_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HCV5528493.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMP
                     EWARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHA
                     WSFAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSG
                     SLTLTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEA
                     AGRLDDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAR
                     EHGFDMRDRVSRQDRTEPERVKSPSRGFSMGF"
     gene            3751..4284
                     /locus_tag="OVW45_004190"
     CDS             3751..4284
                     /locus_tag="OVW45_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528494.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYR
                     DISDGVAKQVRDEVRNEVQKLDVAGLIGERISHDFQRLEESVANMKVNADALNRDIAT
                     IHSNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVT
                     LEDKLVEKPVTGKSRKN"
     gene            complement(4350..4559)
                     /locus_tag="OVW45_004191"
     CDS             complement(4350..4559)
                     /locus_tag="OVW45_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528495.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTD
                     EHVPLYIRRLEYYRLLYRPKQTESY"
BASE COUNT         1276 a         1106 c         1345 g         1436 t
ORIGIN      
        1 aaaaaaaacc cttggaatac aggggcaaaa gtaaggaaaa caaatggatg ctcagtttgt
       61 gtgacacttt aaactaatgg aactatagag tggaggttgt tacagtcgct tttataaaac
      121 agcaaccagt ttttttcaat agtctgatga tggttgtgac agttttgtga tattgttcaa
      181 aatgtttttt gttgaatgtt tgttttttgg tctattggtg tctggtttta ttttttctgc
      241 tattacatat caagaaatgt gattggtgta gcaagagtgg aatgcgatgt ggataaacta
      301 attggttgtc gaatctaatt tacttagaaa actacacttg tttttgtgta tgaatttagt
      361 gaatttgtct gtctatgtgt tttcgtgcat ttataaatcc ttcgtcagga tcttaaattt
      421 agtgttgaat atgtagggcc tgtacggtaa ttattattta gcgtagctac ttcatcgttg
      481 ttcgaacaca accaacaacc tgtaattgtt gtttctcttg gttccccact tttgtggggt
      541 tttttatctc ttccgtgcat cgtaaagcgc ggactccttc tggaaaggtt gttttagggc
      601 atcactgagg cattttttgc gtttgtgaat attgagcatc cgataactaa aatatttccc
      661 atagactctg attcttgcca ccgaccccag gtcggttttt tttgtcctgg cgagatgtga
      721 agtttggaaa caggaaaccc cgtcagagcg ccacgcacca ggggagaaaa cttgtcgttt
      781 tgagccgttc ggtgttcaga acgcacgaaa ccgatcgcac atcgctttcg tgcatagtta
      841 tgcaacgccc tgaaaacgat tctgacgcgt ttttctggtt tggcctggtg ttttcttgtc
      901 tttttgcgtt ttttgcgcca gaaagcgatt gagggcgttt tacatggtac gtacaacggg
      961 agttatggta aatggatcgg atttgcggga aggctcgaca ggttttgtcg ttgggggtag
     1021 tgtaagcgac tgaaaaacaa acgcccctga aattcgtgtc tatccgccaa gactttcacg
     1081 aaaatacagg ggcgatgtcg gtcgataacc tggaaggaat cgtaacacat gagcgccgcg
     1141 cttcaatact tcgaagaaaa tttaccccac cgtccctatc acacggatga tctcgctttc
     1201 ggccttcgta tctccggcaa agggcgcgcg cttcttgcac ggtatatcca gcagaaccag
     1261 cctcatgcac agttctggct ggtttttgac gttgaccgcg agggggctgc gattgactgg
     1321 agcgaccgga acgctcccgc gccgaacatc accgttaaaa atcctgtgaa cggacacgct
     1381 cacctgctct acgcactcaa catcgccgtg agaaccgcgc ctgatgcctc ggttaaggcg
     1441 ctgaaatatg ccgcagcggt tgaacgttcg ctgtgtgaga aactttgtgc ggatgtgaac
     1501 tacagcggcc tgatttgcaa aaatccgttc cacctggaat ggctggtgat ggagtggcgc
     1561 gaggaagcct ataccctcga tgaactggct gattatctcg atttgagcgc ctcagagcgt
     1621 cgtagcatcg ataaacatta cgggatgggg cgaaactgcc acctgttcga aatgacgcgc
     1681 aaatgggctt acagggcgat tcgtcagggc tggccagcgt tctcacagtg gcttgaagcg
     1741 gtgatccagc gtgtcgaaat gtacaatgca tcgcttcccg ttccgctttc actggctgaa
     1801 tgtcgggcta ttggcaagag cattgcgaaa tacacgcaca ggaacttcac gccggaaact
     1861 ttcgcacagt atgtggctga tacgcacacg ccagaaattc aggctgcacg cggtcgcaag
     1921 ggcggttcta agtctaagcg cagcacagta gctacatctg cacgcacgct gaaaccgtgg
     1981 gaggcattag ggatcagtcg cgcctggtat taccaactga aaaaacgagg tctcgtagag
     2041 tagaccaaat aagcctatat cagataacag cgcctttctg gcgtcttttt gagcagcagg
     2101 ttttttgccg ttttcccccg ttcagccctg caatggcgcg gctttccgcg tgattgtggt
     2161 ggtagtgctc gccgcagtct catgaccgag cgtagcgagc gaatgagcga ggaagcacca
     2221 gggaatcgga acacggatgg aggcacttac gcgtctgggt ttggggatgt ctccagatcg
     2281 tacttaagtt ccttaagtgc ttgtaatatc ctgtcttttt tatagcatag ctcgcctgtt
     2341 tttgtgtcta ccagtcctgc ttcgatcagt agcccagccg cgagaaagag ccggtgattg
     2401 cgttcccgtc gttgtttttt tccctcgatt cgtttttgga ccgcctgttt tcgttcgctc
     2461 tcctgtgcct gtaagtcgtt tttttccgct gttgccagta cgtggagaag tttgcgttcc
     2521 tgttctgtga gtccggcttc gttgtcgatc aggtcaataa tctgtctctg tcgtgcggtc
     2581 tgttctccga gcttcctgat gaatccgatc cgtttcttca gccattcgat gttgctgatg
     2641 cgcatgttcg tgatgctccc tggtgttttt ttgcggtgtg ttgtggtcgt gcagtatatc
     2701 gcttgcggtc ggcgggtgca gggggtatct tccgtgctaa ttcgtaaaat tagaaggaag
     2761 cgcacttata tgtttctctc cttcgtcgag aaacagtgcg ctcctgcaga gggctgaaac
     2821 cgtggcaacc tatcacctca gcgttaaatt tggtggcaag gggcaggctg caaaccacgc
     2881 cgattacatc gagcgcaaag agaaataccg cgaccgtcag gatttggaat attccgcaca
     2941 cggaaacatg ccggaatggg ccagggataa tccttcccat ttctggcagg cggctgacca
     3001 gtttgaacgt gcgaacggtt cgacctatag ggaacttgaa attgcattac cccgtgaact
     3061 gacaccggaa cagcgccttg aactggtgca ggattttgta cgtcaggaag ctggcgaacg
     3121 gcacgcctgg agttttgcca ttcacaatcc gaaagcgagc attgacggcg gcgaacagcc
     3181 tcatgcgcat atcatgatga gtcagcgtgt gaatgatggc attgaacgta caccggaaca
     3241 gtatttccgg cgctataacg ccaggtatcc cgagcgggga ggtgcgaaaa aggattccgg
     3301 aagcctgact ctcactcagc aaaaagagca gttgcgggaa ttgcgaaaac gctgggaggt
     3361 gaagcacaac gaacatatga gaaagcatgg ctttgaaagg ggcttcattg actgccggac
     3421 gctgaaagaa cagggcattg aacgacgtcc cgaagtgcat ctgggttttg aagctgccgg
     3481 acggcttgat gatgcccagc gtcatgacat catgcaaaaa cggtctgaac cggactattc
     3541 ccgccatctt gaacaggcgc gaacggtcgg gcatgacatc agggaatggc tggacactcc
     3601 ggttaagcaa catgaactgg tcagaaggct tcaacatgat gttgcgcggg agcatggctt
     3661 tgacatgaga gacagagtga gcagacagga ccgaactgaa cctgaacggg ttaaatcacc
     3721 gtcacgtgga ttttctatgg gattctgaac atggccagca cagaagaaaa attactcacg
     3781 ctcctgttat cggtggagca tctgcaacat accgcaatgg agcaacagaa agctgttgct
     3841 gattctctgg ctgtggcaag ggaaacactt cgcggatatc gggatatttc tgacggtgtg
     3901 gcaaaacagg tcagggatga agtcagaaac gaggtgcaga agctggacgt agcagggctg
     3961 ataggtgagc gcatcagcca tgattttcag cggctggaag agtcagtcgc aaacatgaag
     4021 gtaaacgcgg atgccctgaa cagggatatt gcgacaattc acagcaatat cgtgcaggaa
     4081 tacaacaccc ttaaagggta tagctggaaa tggctgggtg gggtgtcagt ggcttttctg
     4141 gttatgttct gtgtgctcgg atggggaatg aaaacaatac tcgacaggaa ctacaacgcc
     4201 acagcggcgg tgtatcagaa agttgtgaca ctggaagata aactggtgga gaaacctgtc
     4261 acggggaaga gccggaaaaa ctgacgcgct tcgcttgtcc aacgcctcac gacccgcgaa
     4321 aggatttcgc gaaccgttca ccgttttttt caatatgatt ctgtctgctt tggtcgataa
     4381 agcagacgat agtattccag ccttcgtata tataacggta cgtgctcatc agtaagagct
     4441 ggcggtattg tgttatcagg gggaagtttt ttcagatact cacgaaatgc ctggcttgat
     4501 gccataaaat ctacagtagc atcgataata tgtggggtgt tttcacctaa ttttgccatg
     4561 atgataatcc ttatattatc acggtttgtg aatgataggg tgtcactatt atttatgcgt
     4621 tagtgaacca gaaaaaatcc cccgttggca tagggggata tgtaaggaga aataacttcg
     4681 caggtgttct tgtttgtata acgtgaaaag actgcacgca acttaataag tctgccgtac
     4741 ttactttcag ttttcaacat aaggtttata acaatgtatg gaatggaagt gagctttaag
     4801 gtttcagctc taaaaaataa aatatttatt agttggctgg ttgttgttct tgttctgtat
     4861 gtatataaac actatcgaca tattatgacc gggaacattc cttaccccct ctgcgctcgc
     4921 agaggttttt tttgccatta attaataatg aactgaaatg cagttccgat acctccattt
     4981 gcacattatc cctgacatat ggtaccggaa ccgcattaat tttatgttat aagagtttat
     5041 ttttaatgtc cagatggttt aatagtattt atatgtaatt agctgtatgg atagtactgg
     5101 ttttattgta taacttgtcg ttttttttat tgtttttgtg tggtgtgcta atctttaagt
     5161 tat
//
LOCUS       DAKISA010000188         5117 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.191, whole genome shotgun sequence.
ACCESSION   DAKISA010000188 DAKISA010000000
VERSION     DAKISA010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5117
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.191"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(38..691)
                     /gene="ribB"
                     /locus_tag="OVW45_004192"
     CDS             complement(38..691)
                     /gene="ribB"
                     /locus_tag="OVW45_004192"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCV5528496.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(795..943)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1065..1355
                     /gene="ubiK"
                     /locus_tag="OVW45_004193"
     CDS             1065..1355
                     /gene="ubiK"
                     /locus_tag="OVW45_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCV5528497.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLARLEQRMSELENRSTEIKKQPDPETLPPTL"
     gene            1638..2189
                     /locus_tag="OVW45_004194"
     CDS             1638..2189
                     /locus_tag="OVW45_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HCV5528498.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPGKDATPGNVYAQADYKIAYE"
     gene            2249..4753
                     /locus_tag="OVW45_004195"
     CDS             2249..4753
                     /locus_tag="OVW45_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCV5528499.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAWAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYMNYQNEKESYIVTFNQYLESLRLN
                     TYVSLARNTYWDASSNVNYSLSLSRDFDIGPLKNVSTSLTFSRINWEEDNQDQLYLNI
                     SIPWGTSRTLSYGMQRNQDNKISHTASWYDSSDRNNSWSVSASGDNDEFKDMKASLRA
                     SYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDAD
                     GAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVDIENSVITTTLTEG
                     AIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVKDKTSHKELGLVADGGFVYLNGIQD
                     DSKLTLRWGDKSCFIQPPNSSNLTTGTVILPCISQN"
     gene            4769..>5117
                     /locus_tag="OVW45_004196"
     CDS             4769..>5117
                     /locus_tag="OVW45_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCV5528500.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVRE"
BASE COUNT         1541 a         1146 c         1064 g         1366 t
ORIGIN      
        1 cggtttcttg attaaggcag taattaagca gcggttttca gctggcttta cgctcatgtg
       61 cctgacggta tgccaccagg tcttcaatag tcacgagcgc catattgtgt ttattggcaa
      121 actcaatgca ctctggtgca cgcgccatcg tgccatcgtc attagtcagc tcacacagca
      181 caccagccgg tttaaagcct gccagcgtca tcaaatcaat agtcgcttca gtatgaccgc
      241 cacgcgtcag tacaccacct gcctgcgcgc gaagtgggaa aacgtggccc gggcgattca
      301 gatcagaagg ttttgcgcca tcggcaatcg ctgcgcgaac ggtcgtaata cgatcagcgg
      361 cagaaacacc ggtagtcaca ccttcagccg cttcaatggt cacggtaaaa ccggtgccat
      421 aggcgctggt gttattttct accatcattg gcagatcaag ttgtttacgg cgatcttcag
      481 tgatgcacag gcaaacaata ccgctaccgt ggcgaatggt cagcgccatc tgctcaacgg
      541 tcatggtttc tgccgggaag atcatatcgc cttcgttttc acggtcttca tcatcaagca
      601 ccattacacc gcgtccttca cgcagcgcag ccagtgcatt ttcaacacgt tcgaaaggcg
      661 taccaaaaga ggaaagtagc gtctgattca tggtaaaaaa acctcactaa aattatggtt
      721 accagaatca gggcagtctt aggagtggcg gcatatagcc aaaataacgt gagcgggtcc
      781 atgcccgaca gaatcgttac tctctcccat ccggactcta accgtcggcc ccggaattac
      841 accggatctg ctgtcctttg agttcgcacc caaagcgctc gcgggctttc aactgagttg
      901 atttaccgcc ggtggggaat ttcgccccgc cctgagaata agcggattca ctataacgct
      961 aatgattagc ggcagcaacg catagcttca cataattctg gtttatgact tacccttatc
     1021 gcactacaat ggtactcaac acctatcagt acagggaaac tacaatgatt gacccgaaaa
     1081 aaattgagca aatcgctcgc caggttcacg aatcaatgcc taaaggaatc agggagttcg
     1141 gggaagatgt ggagaaaaaa atccgccaaa ccctacaagc tcagctgacg cgcctcgacc
     1201 ttgtaagccg tgaagagttc gacgtccaaa cgcaagtcct gttacggact cgtgagaaac
     1261 tggcgcggct tgaacaaaga atgagcgaac tggaaaatcg cagtactgag attaaaaagc
     1321 agccagatcc agagactctc cccccaacgc tgtaaacatt tgtctttaat gcactgtaaa
     1381 ttatgaatta atcttctaat ttacagtgct ttctattacc ttatcgctta attccattca
     1441 gaatatcatc acaaaaatga atgagtgcaa ttgaaaatat aattgttatg tattttttaa
     1501 gttttactta atccatattt agataaacta atttcataaa acccgaaaag taaaatataa
     1561 tatttgaatg attagcaatc cttaaataac agttactaat cattcatttt ctacttatat
     1621 ttatataaag gaatattatg tctgctttta aaaagtcact tcttgttgct ggcgtagcaa
     1681 tgattctttc caataacgta tttgctgacg aaggtcacgg cattgttaaa tttaaaggtg
     1741 aagttatctc tgctccatgt tcaattaaac caggtgacga agacttaact gtaaatctgg
     1801 gtgaagtagc agataccgtt ctgaaaagcg accagaaatc tttggctgag cctttcacta
     1861 ttcacctgca agactgcatg ttgagccagg gtggaaccac ttattcaaaa gcgaaggtta
     1921 ctttcaccac agcgaatact atgacgggtc agactgacct gctgaaaaac accaaagaaa
     1981 ctgaaatcgg tggtgccaca ggcgttggcg tacgtattct ggatagccag agcggcgaag
     2041 tgactttagg tactccggta gtaattacat tcaacaatac taacagctat caggaactga
     2101 acttcaaagc tcgtatggaa tccccgggca aagatgctac cccgggcaac gtttacgctc
     2161 aggctgacta taagattgct tacgagtaat cacattaggc cgggttcgcc cggccacatc
     2221 attaacggat taatgataag tggaacagat gtataaaaaa ttaaaattaa ccacaataag
     2281 cgaattgatt aaaaatattt attgttcatt atccgttctc atcattggtt gtgcgtcagc
     2341 ttatgccgtt gaattcaaca aagatttaat cgaagccgaa gatcgtgaaa acgttaacct
     2401 ttcccaattt gaaactgatg gccaattacc cgtaggtaaa tactcattaa acgctcttat
     2461 taataataaa aggacgccaa tccatctgga cctgcaatgg gtattaattg ataaccaaac
     2521 tgcagtttgt ctgactccag agcaattaac attattaggc tttactgatg agattattga
     2581 agaggctcag caaaacctga tcgatggttg ttaccctatc gaaaaagaaa aacaaattac
     2641 aacttatctc gataaaggga aaatgcaatt atccatatct gcacctcagg catggttaaa
     2701 atacaaagat gcaaactgga cgcctcctga actttgggat catggtattg cttgggcatt
     2761 tcttgactac aatttatatg cctctcatta tgcaccacat cagggcgata attcacaaaa
     2821 tataagttcc tatgggcagg ctggggttaa tcttggggcc tggcgcctgc gtactgatta
     2881 ccagtacgat cagtcattta acaatggcaa aagccaggcg aacaacctgg attttccgcg
     2941 tatttatttg tttcgcccaa tcccagcaat taatgcaaaa ctaactatag gtcaatacga
     3001 tactgaatcc tctattttcg actctttcca tttttctggc gtttcgttga aaagcgatga
     3061 gaatatgttg ccgcctgatc tacgtggtta cgcaccgcaa atcacgggtg ttgcacaaac
     3121 gaatgcaaag gtcactgtct cacagaacaa ccgtattatt tatcaagaaa atgttcctcc
     3181 tggcccattt gctattacta atttattcaa tacattacag gggcaactgg acgtcaaggt
     3241 tgaagaagag gacgggcaag tcacgcaatg gcaagttgca tctaatagta ttccttatct
     3301 gacgcgtaaa gggcagattc gctacaccac tgctatgggt aaaccgacca gcgttggtgg
     3361 tgattcctta caacaaccct tcttctggac tggtgaattc tcatggggtt ggctgaacaa
     3421 tgtatccctg tatggtggtt cagttttaac aaaccgtgat tatcaatctc tggctgccgg
     3481 cgttggcttt aaccttaact cattgggttc attatctttt gatgtcacac gatctgatgc
     3541 tcagttgcat aatcaggata aagaaacggg ttatagctac cgcgctaact attcaaaacg
     3601 ttttgaatct accggtagcc agctcacttt cgctggttac cgtttctctg ataaaaactt
     3661 tgtgacaatg aatgaatata tcaatgacac taaccattac atgaattatc agaatgaaaa
     3721 agagagttat attgtcacgt ttaaccagta tcttgaatca ttaaggttaa atacatacgt
     3781 aagtttggct cgtaatactt actgggacgc cagcagtaat gtgaattatt cattatcact
     3841 tagccgcgat tttgatatcg ggccattaaa aaacgtctcc acttctctaa catttagccg
     3901 aataaactgg gaagaagaca accaggatca actgtaccta aatatttcga ttccctgggg
     3961 aaccagtaga acattgagct atggtatgca acgaaatcag gataacaaga tttcgcatac
     4021 tgcttcgtgg tatgactctt ccgatcgaaa taattcctgg agcgtttctg cttcaggcga
     4081 caatgatgaa ttcaaagata tgaaagcgtc actacgcgcc agttatcagc ataataccga
     4141 gaacggtcgt ctctatctct ccgggacatc acagcgagac agttattatt ctctgaatgc
     4201 cagttggaat ggttcattca cggcgactcg tcacggagcc gctttccatg actatagtgg
     4261 tagtgctgac tcgcgtttta tgatcgacgc agatggtgca gaagatattc cgttgaacaa
     4321 taaacgcgcg gtaactaatc ggtatggcat cggagttatt ccatcagtca gcagttacat
     4381 aacaacatca ttaagcgttg atacccgaaa tcttccagaa aatgtggata tcgaaaactc
     4441 ggttatcacc accaccttaa ccgaaggtgc tattggctac gccaaacttg atacccgcaa
     4501 gggctatcaa atcatggggg ttattcgcct ggcagatggt agtcacccac cactggggat
     4561 tagcgtaaaa gataaaacca gccataaaga attaggactg gttgctgatg gcggctttgt
     4621 atacctcaac ggcattcagg atgacagcaa acttacttta cgctggggtg ataaatcctg
     4681 ttttattcaa ccacccaata gcagcaactt aaccaccgga acggttattt taccgtgtat
     4741 tagccaaaat taattcagga tgaaactaat gcgctactta aacacaaaga atctcattgc
     4801 agcaggagta ttactcgcct gcatgagcag cattgcctgg ggtgccatca tcccggaccg
     4861 tacccgtatc ataatgaatg agtcagataa aggggaagca ttaaaactta ctaatcaaag
     4921 caaaaatcta ccctatctgg cgcaaacatg gatagaagat acgaaaggca ataaatcacg
     4981 tgactttatt gtcacggttc cccctatggt acgtttaaat cccagtgagc aaattcaaat
     5041 aagaatgata agtcaggaaa aaattgctca actgcctaat gacagagaaa cgctcttcta
     5101 ttttaacgtg cgagaaa
//
LOCUS       DAKISA010000189         5114 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.192, whole genome shotgun sequence.
ACCESSION   DAKISA010000189 DAKISA010000000
VERSION     DAKISA010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.192"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..379
                     /locus_tag="OVW45_004197"
     CDS             <1..379
                     /locus_tag="OVW45_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528501.1"
                     /translation="KKTNIYFDDNFLNIYKRSQDRAPQIISDLKKHNIEIGEKLNDCR
                     KKLNISDVKLTYYWFVTDTGGVDYLDGVLTYNFQGKNRAENFHKQLLQYAYINYSKNQ
                     IPRDFVTSIKNSIYYKIEDCTKF"
     gene            complement(575..2125)
                     /locus_tag="OVW45_004198"
     CDS             complement(575..2125)
                     /locus_tag="OVW45_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HCV5528502.1"
                     /translation="MASYHCTVKAGAKGSALKHADYISRSGEYKSYKSREDLEFSSSG
                     NMPSWAKKNPAELWKAADEFERKNGTAYREIEIALPRELTREQRIELVEDFVQKELGD
                     RHAYQYAIHNPPGAIDGKEQPHAHIMFCERINDGIERDPQQFFKRANSKSPERGGAKK
                     ASIPQTAGERKAALVALRSRWADVQNEHLARHGHESRVDHRSLKEQGINRTPEVHLGP
                     VQAASLNGEQIVAIQERRNAERELKTARDAANAIQQEQEQKQKIKAVEPVRSARSPEL
                     LLQYRKVMKTVIQGEARLARLGDANPNALKEHKLLQNAKAKKDSLSEWSRRIYEGARY
                     LDKLGRNVVSAQRELRELQEQRNALNGIRGLFRGADKREIDARILEQKSVLETAEHER
                     NEFRNKLQQAESEWDKENAAFKRTEGYKYVGELDRYREREILAAASLENTRQKVAEEV
                     SVARSQMLSLEPELSISGDEKAQMRHELLAEMAQERQQQEEKALCIQRSWARGVSRSN
                     ERDQDMER"
     gene            2297..2743
                     /locus_tag="OVW45_004199"
     CDS             2297..2743
                     /locus_tag="OVW45_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HCV5528503.1"
                     /translation="MTNREEWLSAKIAYINGLKSPSEQQRLLVLLAEKKNRTTTDEKT
                     LSALIRAEKTAEKAAAAKARVTAIIAAERKAAARAERKARDHELYKAAGLMIVAGLVD
                     SKTGKPKFSAAELVGALAGIAELPHNHPKWQEWEKRGKELLTKDSA"
     gene            complement(2937..3872)
                     /locus_tag="OVW45_004200"
     CDS             complement(2937..3872)
                     /locus_tag="OVW45_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009440807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCV5528504.1"
                     /translation="MSAALQYFEENLPHRPYHTDELAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDRVGAAIDWSDRNAPAPNITVKNPENGHAHLLYALNLAVRTAPDASV
                     KALKYAAAIERALCEKLDADVNYSGLICKNPFHLEWQVMEWREEAYALDELADYLDLR
                     ASERRSVDKHYGLGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPV
                     PLSPPECRAIGKSIAKYTHRNFTPESFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDK
                     RFMALCMLENGYSQKAIAAMLNVHRNTIRNWAMHK"
     gene            4384..>5114
                     /locus_tag="OVW45_004201"
     CDS             4384..>5114
                     /locus_tag="OVW45_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528505.1"
                     /translation="MGKMNINFKHILPLLSSFFISGCDSDGISKVKSFVYYDIDDSMT
                     VGKAFDTRSDCINGTWSEKEDDRGRTIVTYTCDISESGLKIINSAIMQEPEDKAEYSI
                     AQNNNSIKSTNESLEEYKQKTPLVEKTKEVIIDHIRKLNSAFNEDPMIYSKLTISPYL
                     DRMLYLKDHNDNALNEICGGYEYIQSADSYSWRDVSEEYAKESCEKHIYKVYQSFKKN
                     ASPLITKNFPGFYERVPPCENADEC"
BASE COUNT         1417 a         1244 c         1126 g         1327 t
ORIGIN      
        1 aaaaaaaaca aatatatact ttgatgataa ctttctaaat atctacaaaa gaagccaaga
       61 tagagcacct caaataattt ctgatttaaa aaaacacaat atagaaatag gtgaaaagtt
      121 aaatgattgt aggaaaaaac taaatatctc agatgtcaaa ttaacctatt actggtttgt
      181 aacagatacc ggaggagttg attatttaga tggggtgttg acatataatt ttcagggaaa
      241 gaatagagct gaaaactttc ataaacaact attgcaatat gcatacatta actacagcaa
      301 aaatcaaata cctcgggact ttgtaacatc aataaaaaat agtatttatt ataaaataga
      361 agattgcact aagttttagt ccttccctta caggtggtta cacatataac cacctgcaaa
      421 tatttaacac ctgttaactt cattaatctg aactgtgagt aaatcaaaac gacaattttt
      481 ctcattcaga aaacgtttca cgggacgcgg aattttttcg cgttccgtga aacgttggcg
      541 gggttcgggg cgcagtcccg atattgctac attttcaacg ctccatatct tgatcacgtt
      601 cgttactacg tgataccccc ctggcccagg atcgctgtat acacagtgct ttttcctcct
      661 gttgctgccg ttcctgcgcc atttctgcca gtaactcatg tcgcatttga gctttttcat
      721 ctccagatat actgagttca ggctccagag ataacatctg gctacgggca acggatacct
      781 cttcagcaac cttttgcctg gtgttctcta aagatgccgc cgccagaatc tcgcgttctc
      841 tgtacctgtc cagctcccca acgtacttat atccctcggt acgcttaaaa gcagcgtttt
      901 ctttgtccca ctcggattcg gcttgctgta gcttgttcct gaattcattc ctttcatgtt
      961 cagcggtttc cagcacactt ttttgctcca gaatacgcgc atcaatctcc cttttgtcag
     1021 ccccccgaaa caggccacga atcccattca aagcattgcg ttgttcctgt agctcccgca
     1081 gctccctttg tgcggaaaca acattacgcc ccagtttgtc taaataccgt gccccttcat
     1141 atattcgtct actccattcg gaaagagagt cttttttagc tttcgcattc tgaagcagct
     1201 tatgttcttt gagcgcattg ggattggcat cccctaaacg agcaagcctt gcctctccct
     1261 gaatgaccgt tttcataacc ttgcggtact gaagcaataa ctccggtgaa cgggctgaac
     1321 gaacaggctc aactgccttg attttttgct tttgctcctg ttcctgctga atggcatttg
     1381 cggcatctct ggcggtcttg agttcccgct cggcattcct ccgttcctgg attgcaacaa
     1441 tctgctcccc attcagactg gcggcctgaa cggggccaag atgaacttcc ggcgtgcgat
     1501 tgataccctg ttctttcaga gaacgatgat ctacgcgcga ctcgtgaccg tgtcgcgcaa
     1561 ggtgctcatt ctgcacatct gcccagcggg aacgcagcgc aacaagggcg gcttttcgct
     1621 ctccggctgt ctggggaata ctcgcttttt ttgcgcctcc ccgctctggt gatttgctgt
     1681 tggcacgttt aaaaaattgc tgtggatcgc gttcaatacc atcattaatc cgctcgcaaa
     1741 acatgatatg cgcgtgtggc tgttcctttc cgtcgattgc acctggcgga ttgtggatcg
     1801 cgtactggta ggcatggcga tcgccaagtt ccttttgcac gaaatcttca acaagttcta
     1861 tccgttgttc gcgtgtcagt tcacgcggta acgcgatctc aatttcacgg tatgcagtcc
     1921 cgttttttcg ttcaaattca tctgcggctt tccatagctc tgcgggattt ttcttcgccc
     1981 atgatggcat gttaccggat gacgaaaatt ccagatcctc ccgcgatttg taactcttat
     2041 attcgccgga tctggaaatg taatcagcat gttttaaggc actgcctttt gctccagctt
     2101 tgacagtgca atggtagctt gccatctgcc ttttagccct ccgcaggagc gcacagtttc
     2161 tcgacgaagg agagaaacta ctaagtgcgc atccctgaat taatcatgga tgattttacg
     2221 gtctcacacg caggcaaaca aggtgatatt atgcataata acaactcaca ttaacggcaa
     2281 aagtgcatag gtaagaatga caaatcgtga agagtggtta agtgcaaaaa ttgcctacat
     2341 caacggactg aaatcaccat cagagcagca gagattactt gtgttactgg cagagaaaaa
     2401 aaaccgaaca acgacggatg aaaaaacgtt gtccgcactg atacgggcag aaaaaaccgc
     2461 agagaaagca gccgcagcaa aagccagagt aacggcaatc atagcggcag aacggaaggc
     2521 agccgcaagg gcggaacgaa aggcgcggga tcatgaactc tacaaggccg caggcctcat
     2581 gattgtggca ggactggtgg acagcaaaac cggaaagcca aagtttagtg ctgccgaact
     2641 ggtaggcgca ctcgcaggaa tcgcagagct accgcacaac catccaaaat ggcaggaatg
     2701 ggaaaaacgc gggaaagagc tactcaccaa agacagtgcg taagagcctc cgttcacgtt
     2761 ccagttccct ggtgcttcct cgctcattcg ctcgctacgc tcggtcatga gactgcggcg
     2821 agcacgacca cctcaatcat acggtaagcc gcaccattgc tgggctgaaa ggagaaaacc
     2881 gccggaaagc ccgctgctca aaaagacgcc gaaaaggcgc tgttatctga tataggctat
     2941 ttgtgcattg cccaattacg gatcgtatta cgatgtacat tcagcatcgc cgcaatagct
     3001 ttctgagaat atccattctc cagcatacat agcgccatga aacgcttgtc atcgtaggct
     3061 tcgcctttag cgatgccacc tttccggcct ctcttggcct gtatttctgg tgtgtgcgta
     3121 tcagccacat actgtgcgaa actttccggc gtgaagttcc tgtgcgtgta tttcgcaatg
     3181 ctcttgccaa tagcccgaca ttcaggcgga gaaagcggaa cgggaagcga tgcgttgtac
     3241 atttcgacac gctgaatcac agcatcaagc cactgtgaga atgctggcca gccctgccga
     3301 atcgccctgt aagcccattt gcgcgtcatt tcgaacagat ggcagtttcg ccccagcccg
     3361 taatgtttat cgacgctacg acgctctgag gctctcaaat cgagataatc agccagttca
     3421 tcgagggcgt aggcttcctc gcgccactcc atcacctgcc attccaggtg gaacggattt
     3481 ttgcaaatca gtccgctgta gttcacatct gcgtcaagtt tctcacacag cgcacgttca
     3541 attgcagcgg cgtatttcag cgccttaacc gacgcatcag gcgcggttct cacggcaaga
     3601 ttgagcgcgt agagcaggtg agcgtgcccg ttctcgggat tttttacggt gatgttcggc
     3661 gcgggggcgt tccggtcgct ccagtcaatc gcagctccta cgcggtcaac gtcaaaaacc
     3721 agccagaact gcgcatgagg ctgattttgc tggatgtagc gagcaagcag cgcacgccct
     3781 ttgccggaga tacgaagacc gaaagcgagc tcatccgtgt gatagggacg gtgaggtaaa
     3841 ttttcttcga agtattgaag cgcggcgctc atgtgttacg attccttcca ggttatcgac
     3901 cgacatcgcc cctgtatctt cgtgaaagtc ttgccggata gacacgaatt tcaggggcgt
     3961 ttgtttttca gtcgcttaca ctacccccaa cgacaaaacc tgtcgagtct tcccgcaaac
     4021 ccgatccatt taccataact cccgttgtgc gcccccctta aaacgccctc agtcgctttc
     4081 tgtcgcaaaa aacgcaaaaa gacagggaaa caacaggcca gaccaaaaaa acgcgccaga
     4141 attgttttca gggggctgca taactatgca cgaaagcgat tcatgtccgg tttcactcgt
     4201 tttgaacagc gaacaggtca aaacagcaaa ctttctcatc cagaaaaagg tgaactggtg
     4261 cgaaatcttt tcgaactgcg taagacgttg agcacaaaca tagtaagtcg gtttatcggt
     4321 atgtatggat tccattaaca tatcgcctgt attaatattt cctcgttatt gttagaaata
     4381 aaaatgggaa aaatgaatat taactttaaa catatccttc ctttattgtc gtcatttttt
     4441 atttcaggat gcgactctga tggaataagc aaggtaaagt ctttcgtata ttacgacatt
     4501 gacgactcaa tgactgttgg taaagccttc gatacaagga gtgattgcat caatggcaca
     4561 tggagtgaaa aagaggatga tcgaggaaga accatcgtca catatacctg tgatattagt
     4621 gaaagtggcc taaaaataat aaattcagcc attatgcaag aacctgaaga taaggctgaa
     4681 tattctatag cacaaaataa caacagcata aaatcaacca atgaatcact agaggaatac
     4741 aaacagaaaa cgcctctggt ggaaaaaaca aaagaggtca taatagatca tataagaaag
     4801 ctaaacagtg cttttaatga agatccaatg atatatagca aactcacaat tagcccttat
     4861 ttagacagaa tgctatatct aaaggaccat aatgacaatg cgctaaatga aatttgtggt
     4921 gggtacgaat acattcaatc tgccgatagt tacagttggc gtgatgtaag tgaagagtat
     4981 gcaaaagaat catgcgaaaa gcatatatat aaagtatatc aatcgtttaa gaaaaatgca
     5041 tcaccattaa taactaaaaa cttccccggt ttctacgaac gtgtcccccc ctgtgagaac
     5101 gcagatgaat gcat
//
LOCUS       DAKISA010000190         5034 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.193, whole genome shotgun sequence.
ACCESSION   DAKISA010000190 DAKISA010000000
VERSION     DAKISA010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5034
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.193"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(144..2213)
                     /gene="glyS"
                     /locus_tag="OVW45_004202"
     CDS             complement(144..2213)
                     /gene="glyS"
                     /locus_tag="OVW45_004202"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCV5528506.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2223..3134)
                     /gene="glyQ"
                     /locus_tag="OVW45_004203"
     CDS             complement(2223..3134)
                     /gene="glyQ"
                     /locus_tag="OVW45_004203"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCV5528507.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3229..3528)
                     /locus_tag="OVW45_004204"
     CDS             complement(3229..3528)
                     /locus_tag="OVW45_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCV5528508.1"
                     /translation="MMMNAFFPAMALMVLVGWSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3703..4698
                     /gene="wecH"
                     /locus_tag="OVW45_004205"
     CDS             3703..4698
                     /gene="wecH"
                     /locus_tag="OVW45_004205"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCV5528509.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4740..>5034)
                     /locus_tag="OVW45_004206"
     CDS             complement(4740..>5034)
                     /locus_tag="OVW45_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528510.1"
                     /translation="VLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMV
                     GLWNATLLLSEKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
BASE COUNT         1182 a         1305 c         1260 g         1287 t
ORIGIN      
        1 tccttgatat aatcctaaaa aaacctcata aaattaatat atgagataat ctttattcaa
       61 cagaagatta ttaaaggttg ctgtattatt tagcgataat aaaaaaagcc tgccagatgg
      121 caggcttttt aataacggcg ttattactgc aacagcgaaa tatccgcaac gcgcaggaac
      181 agttcgcgca gtttctccag catggtcaga cggttgagac gcaattcttt gtcatcaacc
      241 atgaccatca ctttatcgaa gaaagcatca accggctcac gcagctctgc cagttcgacc
      301 agcgcatcct ggtaacgacc ttccgcaaag tacggctcca gcttgtcacg tagcaccaca
      361 acctgcatcg ccagtttaat ttcttccggc tctttcaggg tagaggcatt cacgcggtcg
      421 ctcagcactt cgtcagattt cgccagaatg ttagatacac gtttgttcgc cgccgccagt
      481 gcagcagctg catccagggt acggaaatgc gataccgctt tcatacgggc atcgaaatca
      541 gccggacgag tcggacgacg cgccagtacc gcctggatgg tgtcaacggt gtaaccttcg
      601 tcctgatacc aggcgcggaa gcgaccgagc ataaagtcga taacatcatc aactacgttg
      661 gcgttagtca gcttatcgcc atacagacgc actgcttctt cggtcagcgt ttgcagatca
      721 aggttgaggt tcttctcaac gataattcgc agcacgccaa gtgcggcacg acgcagcgca
      781 aacgggtctt tgtcgccttt cggatgctga ccgataccga agatacccgc cagagtatcc
      841 atcttatcag caatcgccag cgcacaagct accgggttgg acggcaggtc atcaccggca
      901 aagcgcggct gatactgctc attcagcgcg acggctacgt cttccgcttc gccatcgtga
      961 cgcgcgtagt gcatccccat aacgccttgg gtgtcggtga actcgaagac catgttggtc
     1021 atcaggtcac acttagacag caggcccgca cgggttgcgt ggttaacgtc agcgccaatc
     1081 tgttcagcaa tccagccagc cagcgcctgg atgcggtcag ttttgtcacg cagcgtaccc
     1141 agctgttgct ggaacagcac ggtttgcagg cgcggcaggt tatcttccag acgtttttta
     1201 cggtcggtat tgaagaagaa ctcggcatcc gccagacgtg gacgaacgac tttctcgtta
     1261 ccagagataa tttgctgcgg atctttcgat tcgatgttgg caacgaagat gaagttcggc
     1321 agcagtttgc catcgttcgc atacaccggg aagtatttct ggtcaccttt catggtgtaa
     1381 accagggctt cagccggcac cgcgaggaat ttctcttcga attttgcggt cagaacgacc
     1441 ggccactcca ccagcgaagc cacttcttcc agcaggcttt cgcttaagtc agcgttaccg
     1501 ccaatcttac gcgctgcttc ttcggcatcg gctttaatct tcgctttacg ttcttcgtaa
     1561 tcggcgatga ctttcccgcg ctcacgcaga atttccggat actgatcggc gttgtcgatg
     1621 gtgaattccg gttcgcccat aaagcggtgg ccgcgaatca cgcgatcgga ctgaatgccc
     1681 agaatggttg ccggaatgac tttgtcgccc agcagcaggg tcacggtgtg caccggacgc
     1741 acgaagtgca cgtcgcttgc gccccaacgc atcagtttcg ggatcggcag tttcgccagc
     1801 gaagtcgcaa ccatattcgg cagtagtgct tcagtgcttt cgcccttcac atgggcgcga
     1861 tacagcagcc attcgccttt atcggtagtc agacgctcag cctggtcaac ggtaataccg
     1921 caaccacgcg cccaaccttc tgccgctttg ctcggtttgc cttcagcgtc gaacgcctgg
     1981 gcaatcgccg ggccgcgttt ttcgatttca cgatctggtt gcgcttccgc caggttagct
     2041 actttcaacg ccagacgacg cggagcagca aaccattgaa cggtgccgtg tgcgaggcca
     2101 gcgttatcca gctccgcagt aaagttcgca gcaaaggact cagccaggct gcgcagtgct
     2161 tttggtggca gctcttcagt gccgatttcc accagaaaag ttttctcaga catagccgcc
     2221 tcttacttat ctttgttgca catcgggaag ccgagggctt cacgggaagc gtagtatgct
     2281 tctgccactg ctttggtcag ggtgcgaatg cgcagaatgt agcgctgacg ctcagtgacg
     2341 gagatggctt tacgcgcatc cagcaggttg aagctgtggg cggctttcag aatacgctcg
     2401 taggctggca gcggcagcgg attttccagc gccagcagct gctgcgcttc tttctcgtac
     2461 tgctcgaagc aggtgaacag gaaatccaca tccgcgtatt cgaagttgta agtggactgc
     2521 tccacttcgt tctgatggaa cacgtcgccg taggtggttt tacccagcgg gccgtcgctc
     2581 cagaccaggt cgtaaacgct gtctacgccc tgaatgtaca tggccagacg ttccagaccg
     2641 taggtgatct cgccggtaac cggtttacac tccagaccac caacctgctg gaagtaagtg
     2701 aactgcgtca cttccatgcc gtttagccac acttcccagc ccagtcccca ggcacccaga
     2761 gtcgggtttt cccagttatc ttccacgaaa cggatgtcgt gaatagtcgg gtccatgccc
     2821 agctctttca gagaaccgag gtacagctcc tgaatattgt ccggcgatgg cttaatgacc
     2881 acctggaact gatagtagtg ctgtaaacgg ttggggtttt cgccgtagcg accatcggtc
     2941 gggcgacgag aaggctgaac ataagcagcc gccatcggtt ctggccccag cgcgcgcaga
     3001 caggtcattg ggtgagaggt tcccgcgccg acttccatgt ccaatggttg aacaatggtg
     3061 cagccctggc gagcccagta atcctgtaag gtcaggatca agccctggaa ggtcctggta
     3121 tcaaactttt gcatattatt tcgtgctgga tacgtgtgga tttaaaggaa gggatcagta
     3181 tacccgctgg atggaagata tacagtacga aacgggaaaa agcagggctt aacgcatgga
     3241 aagatgcaaa aaatggccat ctgcgtcaaa agaacagaca aactgctctt tacggaaggt
     3301 ataaccgcgc atttcatagc ttccctggaa ctgttcgaag gcggtgacgt cgattttttg
     3361 ctcgccggtg ttataacgtt ttgccgcctg atccttgcat aacgcttcca tattcaacga
     3421 acgcagagga tcaaccttta cccgttgtgc tttctgcacg ggtgacggtg tagaccaacc
     3481 cactagcacc ataagcgcca ttgccggaaa gaaagcgttc atcatcatta aattaccgtc
     3541 tggtttgcag gtgagtctta ttatttatat cggtatagtg gctgatatct tacgaatttc
     3601 cttaagcctc tataagctgt agtgataaaa ctcagattat tccagtgcgc caaccaaatg
     3661 atttatcggt cagacttttt ttaaccagat acagagacac agatgcagcc caaaatttac
     3721 tggattgata acctgcgagg gatagcgtgt ttaatggtgg tgatgattca caccactacc
     3781 tggtatgtga ccaatgctca tagtgttagc cccgtcacat gggatatcgc caatgttctg
     3841 aattctgcct ctcgtgtcag cgtgccgcta tttttcatga tttccggcta tctctttttt
     3901 ggcgaacgca gcgcccagcc gcgccatttc ttgcgtatcg gcttatgtct gattttttat
     3961 agcgcaatcg cactgctcta cattgcgctg tttacctcca tcaatatgga gttagcgctg
     4021 aaaaacctgc tgcaaaagcc agtgttttac cacttgtggt ttttcttcgc gattgcggtg
     4081 atttatctgg tttcaccgct gattcaggtg aagaacgtcg gcggaaaaat gttgctggtg
     4141 ctaatggcgg tgattggcat tatcgctaac ccaaacacag tgccgcagaa aattgacggt
     4201 tttgaatggc tgccaattaa cttatatatc aatggcgata ctttttacta cattctgtat
     4261 ggcatgttgg gccgcgctat agggatgatg gacacacagc ataaagcact gtcgtgggtg
     4321 agcgccgcgc tgtttgcgac gggggttttt attatctctc gcgggacatt atatgaattg
     4381 cagtggcgcg gaaattttgc cgatacctgg tatctttact gtgggccgat ggtttttatc
     4441 tgcgcaatcg cgctattgac tctggttaaa aacacgctgg atacgcgtac cattcgcgga
     4501 cttggcttaa tctcccgcca ttcattgggt atatacggat tccacgcctt gattatccat
     4561 gcgctgcgca cccggggaat tgagcttaaa aattggccaa tactggatat tatttggatc
     4621 ttttgcgcga cgttggcagc gagtttgtta ctttctatgc tggtacaacg aatcgacaga
     4681 aacagattag tgagttaagt aaaagcccgt tcacattgga ctgaccgggc ttacgtgaat
     4741 tattcgctgt attcttcctg ggtaacctgt gtttcttttg gcgggtttat tccggcatca
     4801 cgaatattct tctgcaccgc tactgcacca aacaagctta agaagaaagc cagtccataa
     4861 aaaccttttt cactgagtaa taatgtcgca ttccacagac ctaccatcag taatgcaaca
     4921 gagataataa agacagtcag gcaggtcata taataaatgg aagtggtcgg tatgccttca
     4981 tacttgtccc gaacggtctt ttgataagac gcagcagaaa acagtcctaa cacc
//
LOCUS       DAKISA010000191         4868 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.194, whole genome shotgun sequence.
ACCESSION   DAKISA010000191 DAKISA010000000
VERSION     DAKISA010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.194"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            63..782
                     /locus_tag="OVW45_004207"
     CDS             63..782
                     /locus_tag="OVW45_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001470794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vimentin yjdA"
                     /protein_id="HCV5528511.1"
                     /translation="MKFSDRREALKLMTQIESTVTSLHREAEAQFRPELEKIVRGIET
                     GFRGTALYATENIAGRINARLEDEGFTVKITFPAVSQLQTRIAVKTNLSALMEERTET
                     VTRRRRQSGFWGKICGAFGTSDWGWENYKENVSRSVININSVRKEVMSLTRAYFGELQ
                     ASIEQNINQPVRQEIDDFFCTFREKVEQLRNTLIQSSEDHKRDQQAQEQLTERLQALN
                     ERVPELITDSKALREELETLL"
     gene            779..1684
                     /locus_tag="OVW45_004208"
     CDS             779..1684
                     /locus_tag="OVW45_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HCV5528512.1"
                     /translation="MTSPFIQQIADNRVCQVLSCLPEKFVVDFANGIDVAQEHNRTAG
                     GRTFFRRLKEGLTGKGAVRQNAINASLAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLAEQVHQKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     NGGSATVRLNTLDWLGGQSREQADNEWHEAVNWLGDWCSEERHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGEA"
     gene            1681..2751
                     /locus_tag="OVW45_004209"
     CDS             1681..2751
                     /locus_tag="OVW45_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HCV5528513.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGAQISMVSGASIG
                     ALNGAIITASPDLSEAALRLEALWDHLGNNQVLSVNRSVYFSLLKKLVQAMNLCQIPG
                     RAGALLTTLFRHISTINGFDNPMIQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGAGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMYGDGGMGGWRNRQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRRAYPDTTILEIRPR
                     KRLKQIGDEGKSGGLLSFTSAHTDAWRQQGYEDTMLTMEHIRKPLAARQALTRSETVL
                     QKSLEITEGADSALRNAMARIK"
     gene            2883..3341
                     /locus_tag="OVW45_004210"
                     /pseudo
     CDS             2883..3341
                     /locus_tag="OVW45_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771527.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3467..4006
                     /locus_tag="OVW45_004211"
     CDS             3467..4006
                     /locus_tag="OVW45_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HCV5528514.1"
                     /translation="MNWHYEKNGVRYDNVTEEDIIERIRRGELNASTLVWQQGMLEWQ
                     PVSATSLAEILKQSEVPPALPGHRIPGGVVWTLAFAPLIGYILEMWTAGLNGMSFERA
                     YDAVSEGQYWFISLILNIALGYLDERKLRKSGVDTATFGWLAWLIPVYLWRRAKILGQ
                     KPAYFWVWLVMLILVLFTV"
     gene            4276..4689
                     /locus_tag="OVW45_004212"
     CDS             4276..4689
                     /locus_tag="OVW45_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528515.1"
                     /translation="MKNIITILMIAVISFFTANVSANNAVPLPTNLTDVTDGTEFWCQ
                     GTDTIDGGCEYLGTSKDMQYGLVHAMGGTACWDGLYDVINFYTGKAQTIKYNNNQSCE
                     GNITAGFVTLKNGKPGVKLYDNTIHKIVGLDEIKI"
BASE COUNT         1335 a         1048 c         1255 g         1230 t
ORIGIN      
        1 ttagtgtcag tgccccgggc catcattcat ggaaacgaga ctttgatccg gacagccccg
       61 agataaaatt cagtgatcgc agggaagccc ttaaactgat gacgcaaatc gaatcgaccg
      121 tgaccagcct gcaccgtgag gctgaagcac agttccggcc tgaactggaa aaaatcgtca
      181 ggggtatcga aacaggtttt cgtggtacgg ccctgtatgc cacagaaaat attgccggac
      241 gtatcaatgc ccgcctggag gacgagggct tcactgtaaa aatcactttc ccggcagtca
      301 gccagttaca gaccaggatc gcggtaaaaa caaatctgag tgcgcttatg gaggaaagaa
      361 ctgagacagt cacccgtcgc cgtcggcaga gtggtttctg gggaaagatt tgtggagcgt
      421 ttggcaccag tgactggggc tgggaaaact ataaggagaa tgtgagccgc agtgtgatca
      481 atatcaactc ggtcaggaag gaagtgatgt cactgacccg ggcatatttc ggggagttgc
      541 aggcatccat tgagcagaat attaaccagc ccgtccgcca ggagatcgat gattttttct
      601 gtacattcag ggaaaaagtc gaacagctac gtaacacact cattcagagc tctgaagatc
      661 ataaacgcga tcagcaggca caggagcagc ttacggagcg ccttcaggca ttaaatgaaa
      721 gggttcccga gctgattact gacagtaagg cgttaaggga agaactggag acactgctgt
      781 gacctcacca tttattcaac aaattgctga taaccgggta tgtcaggtac tcagttgcct
      841 tcctgaaaaa tttgtggttg attttgcaaa cggtattgat gttgcacagg agcataaccg
      901 cacggccggg gggcgcacgt ttttccggcg tttaaaagaa ggtctgactg gcaaaggcgc
      961 tgtacgccag aatgccatca atgcttcgct tgcacagggc gttgaggcgt ccctgcgctg
     1021 gctgacggag ctgacaacgt cgcttgccac cacaaattac gcgattaccc gggtaaacga
     1081 cagggtcagt tcactggtca gtgatactgc caggctggcg cattattcgg cagacacgcg
     1141 ggagcagtta ctcaccctgg cagaacaggt tcatcagaaa ctgaatcatc ttgaagaaaa
     1201 actccaccgt gttgaccagg ttcagcgggc acagttacat cttgagcaga tattctcatg
     1261 gtggagcgcc gggcgatacg cgtcattttc ccctgccgga cgttgttatg tggcgcttga
     1321 agagcttcgc tggggcgcgt ttggtgatgt gatacgtcag ggtgaaacag gccaggttaa
     1381 ccagttactg gatatcctca gacataaagc attaacgcaa atggcacagg agaatggcgg
     1441 tagcgcaaca gtacgtctga acacactgga ctggcttggt ggtcagagcc gggaacaggc
     1501 tgataacgaa tggcatgaag cggttaactg gctgggggac tggtgcagtg aagagcggca
     1561 tcctgtgatc tggtcaacca cacaggctgc agaacactta ccggttcgta tgccccgtct
     1621 ctgctctgca gaacgcctct ctgaaagtat ggttgatgaa atatttcaga agggggaggc
     1681 atgagtacag aaatgaaaac ggggctggtg ctgtccggcg gcggggcggt gggcgcttat
     1741 caggcgggag tggttaaggc actggcagag tgcggcgcac agatcagtat ggtctcagga
     1801 gccagtatcg gggctctcaa tggtgccatt atcacggcct ctcccgatct gtcagaagcc
     1861 gccttacgcc tggaggcgct ctgggatcat ctggggaata atcaggttct gtcggtaaac
     1921 agatcggttt acttttcatt gctgaaaaaa ctggttcagg ccatgaacct ctgccagatc
     1981 cccggacgtg cgggcgcact gcttacgacg ctttttcgcc atatatcgac aatcaacggg
     2041 ttcgacaatc cgatgattca gccgctgttg tcagatgagc ccctgacagc gctgatggat
     2101 cattatcttg atacagatgc tctggccgac gggctaccgc tgtacgtgtc gctgtacccc
     2161 acagaaggag gcatgcagga tattattgac tgcattcgtg ctgaactggg tgccggaacc
     2221 acgaaaaacg ctgtttttca gcatatccag agtctgcccc gcgggcagca gaaagaagcc
     2281 ttactggcgt cagccgcgct gcctctgctg ttccgtcccc gcgaggttca gggaacaatg
     2341 tacggtgatg gtgggatggg cggatggcga aacaggcagg ggaatactcc cgtgacgcca
     2401 ctggtggatg ccggatgcaa tatggtgatt gtgacgcatc tgagtgacgg ttctttatgg
     2461 gatcgtcggg cttatccgga caccacaatc cttgaaatcc gtccccggaa aaggttgaaa
     2521 caaatcgggg acgaaggcaa aagtggcggt ctgctcagtt ttacatcggc acataccgac
     2581 gcctggcgtc agcagggcta cgaggacacg atgctgacga tggagcatat ccggaaaccg
     2641 ctggcagcac gtcaggcact gacccggtca gagacggtat tgcagaaaag cctggagata
     2701 actgaaggtg cagattcggc actgagaaac gcgatggccc ggattaaata aagacgctcc
     2761 ggagaaacgc caccgccact gtggtggtgt ttttcctgtc tggtattatt gaaaatattt
     2821 tcagatatat attacctgga tgatggttaa gattaattta tttaattttg gggagaaaac
     2881 tcatgaaact gttaaacatt cttttagctg ttgttttaac tgtaagtgca accgtagcac
     2941 aaacagaatc ggcattgtag acgcgtaaca gtattgctgg cactgatatg ttcagtatcg
     3001 gtaatgtttc tattaactgc acgattaatc cagaagacaa caatttgcta ccacactatg
     3061 tgtggattta cggtcatacc tccacccagc taaatcagta cgattctgat attattttta
     3121 tcatcaacga taaaaagtat ccggccccat ctgtcgacgg aacacgaatg aataagaatg
     3181 catgggtata ctttatcgat gctatcagtg aagccacgca attcgacgtt ttagtaaacg
     3241 gtaagaaagt cgacagctat accgcaaata tcaaaaatgt gaaaaagact ttgggtaaca
     3301 aattctacga cagctgctgg aacacctggt tccaggaata attgataacg tggtgtaaaa
     3361 cacctcagtt taatttaata caatgagtga aaatgaatat tataaaaata ttttttgttt
     3421 cagatgtcat atattttatt aactgaatga ttaacaggag aatcagatga actggcatta
     3481 tgaaaaaaat ggtgtacgtt atgataacgt aacagaggaa gatattatcg aacgtatccg
     3541 gcgtggtgaa ctgaatgcat caacactggt gtggcaacag ggcatgctgg aatggcagcc
     3601 agtttcagcc acatcactgg cggaaatact gaaacaaagt gaagttcccc cagcccttcc
     3661 gggtcaccgc attcccggag gcgttgtctg gacgctggca tttgcccctt tgattgggta
     3721 catacttgag atgtggactg cagggctgaa cggtatgtca tttgaacggg cttatgatgc
     3781 agtcagcgaa ggccagtact ggtttatctc tctgattctg aatattgctc tgggatatct
     3841 ggatgaacga aaactccgta aatctggtgt tgatactgca acatttggct ggctggcatg
     3901 gttgatcccg gtttatctgt ggcgtcgggc aaaaatcctc ggacaaaaac ctgcgtattt
     3961 ctgggtctgg ttggttatgc tgatactggt gttatttacg gtctgatatt ctttatcgca
     4021 gagtttacta aataatcagg ttattagtgt tcttaacagg taaatgatgt tcatcatgga
     4081 acggatatat tttatttgat tcaggagaca gtcagaaaca tagtataaat atatcaccat
     4141 gttgaaggtc atatcttcag gtatgtgttt aataaacaag tcgggagtta atcctgacct
     4201 ctcgagtgga ttataagtat tgcagaaaca gtttttatac ttttatttaa tatcagaaac
     4261 aatggagtat ttatcatgaa aaatatcatc actattttaa tgattgcagt tatcagtttc
     4321 tttactgcta atgtatcagc aaacaatgcc gttcctttac cgaccaacct gactgacgtt
     4381 actgacggta cagaattttg gtgtcagggt actgatacta tcgacggagg ttgtgaatac
     4441 ctgggcacat ccaaagacat gcaatacggt ctggtacacg ccatgggggg tacagcgtgc
     4501 tgggatggtt tatacgacgt aattaatttc tataccggta aagcccagac tatcaaatac
     4561 aacaacaatc aaagttgtga aggcaacatc accgccggtt tcgttaccct aaaaaacggt
     4621 aaaccgggcg ttaaacttta cgacaatacg attcataaga ttgtcgggct tgacgaaatc
     4681 aaaatttaat gtatgaatta tagtatctta tctgcgcgtt attaatatca aatgtaaata
     4741 aatcctctgc cataatggca ggggctttat ttttacctga aaatacatga cgggctgtac
     4801 attactttta ttttccgaag atcttttctc acgcaaacag acagttgctg tcataacagc
     4861 ttaccgca
//
LOCUS       DAKISA010000192         4844 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.195, whole genome shotgun sequence.
ACCESSION   DAKISA010000192 DAKISA010000000
VERSION     DAKISA010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4844
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.195"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(34..954)
                     /locus_tag="OVW45_004213"
     CDS             complement(34..954)
                     /locus_tag="OVW45_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCV5528516.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITFDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1092..1991
                     /gene="pgrR"
                     /locus_tag="OVW45_004214"
     CDS             1092..1991
                     /gene="pgrR"
                     /locus_tag="OVW45_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCV5528517.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            2328..3941
                     /gene="mppA"
                     /locus_tag="OVW45_004215"
     CDS             2328..3941
                     /gene="mppA"
                     /locus_tag="OVW45_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCV5528518.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(3992..>4844)
                     /gene="ynaI"
                     /locus_tag="OVW45_004216"
     CDS             complement(3992..>4844)
                     /gene="ynaI"
                     /locus_tag="OVW45_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCV5528519.1"
                     /translation="INYVIENYKLKFITPDVIDFICTSLIAVILTIKLFLLINQFEKQ
                     QIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKD
                     ILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSL
                     FSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYF
                     NQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNI
                     TPPEQGR"
BASE COUNT         1308 a         1081 c         1140 g         1315 t
ORIGIN      
        1 ccatatcact attgcggatt tgaatatgtt tcattacaga gtttcctcaa agaacggagc
       61 taatactgaa attgcttcag ccacataggc tttgccgtca taaagatcca tatggtttgc
      121 gccttcaaca atgtgatagc gtttatcttg gcttgatgct cgatcgtaca ggtcgtcact
      181 catccatttg ctccctgcct ggctgcccgc cacaatctgc atcggctgag tcaggtacac
      241 ttccgccata tggtaagcat caaaggtaat aatctggtta aggctgcgca aagtagcgta
      301 acccggtgct gttggatact gcgcgcgagg ggtgtggtaa tactcccagg cctgacgcag
      361 ttcttcgttc ggcgcatcgg actccttcat tggtgccagt ggcataacgg catattctcc
      421 gctgctaata tcgctggttc gggcgtttga acccgcttca acgtatggca gggcatcaat
      481 agatttcaca ttgttttccc aaccattacg gaacatcgaa ccaatattga ccgcactaac
      541 ggtaccgatg gccttgatgc ggcgatcctg aattgcagca ttggctgtat atcctgcacc
      601 ggcacaaatt cccatcgcac caattcgggt attgtcgaca taagaaagcg ttgtcaggta
      661 atcaatcacg gcactgatgt cttcagtacg aatgtatggg ttttctaact gacgcggctc
      721 gccgccactt tcaccttgat aagatgcgtc ataagcaata gtgacaaatc ccttttccgc
      781 cagttttttg gcataggttc cggccgtttg ttctttaacg cccccacctg ggtgagatac
      841 cacaattgcc tgatactgac gggtttcatc aaattttgga gggaaataga tcactgcaga
      901 caaagagatg gttggattat tgctgttagt gaagctgact ttattattca tcattccgtt
      961 cctcatggag ttgtcggttc gttttaacgg ttggtgatat cactatagat attgatcatt
     1021 aagttgatta gacccaaaat catgattaga cctatcattt aaatgattaa tagattgcat
     1081 ggagatactg aatgaagcgt gaagaaatcg ctgatctgat ggcgtttgtc gtcgttgcag
     1141 aggagcgtag cttcactcgt gcagcagccc gcctgagcat ggcgcagtca gctttaagcc
     1201 agatagtgcg tcgtatagaa gaacgattgg gattgcggct tctgacgcga accacgcgca
     1261 gcgttgttcc aactgaagcg ggcgagcatc ttttgtctgt tcttggccct atgttgcatg
     1321 acatagattc agccctggca tccctgagcg agctgcagaa ccgcccatcc gggacaatac
     1381 gtattactac tgtagaacat gcagccaaaa cgatattgtt accagcaatg cgcacattcc
     1441 tgaaatcgca tcctgaaatt gatattcagc tcaccattga ttatggtttg accgatgtcg
     1501 tttctgaacg ttttgatgca ggcgtccgtc tgggtgggga gatggataaa gatatgatcg
     1561 ccattcgaat cgggccagat ataccaatgg ctattgttgg ctcaccggat tatttttctc
     1621 gccgaagtgt tccaacgtca gtgtcacaat taatagatca tcaggcaatt aatttgtatc
     1681 ttcccacatc gggtacagca aatcgctgga gattaatacg cggtggacgt gaagttcgtg
     1741 ttcgcatgga aggtcagctt ttactgaata cgatagacct gatcattgat gctgcaattg
     1801 atgggcatgg attggcgtat ctaccttatg atcaggttga gcgggctatt aaagaaaaaa
     1861 aactgatacg tgttttggat aaattcacac cagatttacc cggttatcac ctgtactatc
     1921 cacaccgtcg acatgctggc tcggcattct cattatttat agataggctg aagtataaag
     1981 gtgctgttta gcactacttg ctgatacatt aatttaattt ttctcttaac gtattctcag
     2041 ttcctttcaa cgttttggtt attttttatt cttcgtacaa tggcgacaga tgctgattat
     2101 gataccgaaa actggtttga acgtgcgaag cctgaacgta gtgttcggtg ttctatgtgc
     2161 tttaccgtat tttgtcgtct attatttaca ctaaatctga tttaatatat tgatacataa
     2221 aacattttat gctccctgtg tcactttttt gatggaagtt gtttgcattt ctttaaggcg
     2281 aaacaaataa ttacgcatca attttaatgt cggttagagg gaaacttatg aagcactctg
     2341 tttcagtcac gtgttgtgcg ctgttggtca gcagcatttc tctttcgtat gctgcagaag
     2401 ttccgagcgg cacagtactg gcagagaagc aggagctggt gcgccacatt aaagatgagc
     2461 ctgcgtcgct ggatcccgct aaagccgtgg gcctgccaga gattcaggtc attcgcgatc
     2521 tgtttgaagg actggtgaat cagaacgaga aaggggagat tgtccccggc gttgcgactc
     2581 agtggaaaag taatgacaac cgtatctgga cttttaccct gcgcgataac gcaaaatggg
     2641 cggatggcac accggtaacg gcgcaagatt ttgtctacag ctggcagcgt ctggtggacc
     2701 cgaaaacgct atcgccattt gcatggtttg ccgcgctggc gggaatcaac aacgcacagg
     2761 cgattattga tggtaaagct acgccagacc agcttggcgt caccgcagtt gatgcccata
     2821 ctttgaaaat tcagcttgat aaaccgttgc cgtggtttgt gaatttaacc gctaactttg
     2881 ccttcttccc ggtgcaaaaa gccaacgtag aaagcggtaa agagtggacg aaacccggaa
     2941 atctgatcgg caatggcgct tatgttctta aagatcgcgt agtcaatgaa aaactggtcg
     3001 tggtaccgaa tacccattat tgggataacg ccaaaacggt actgcaaaaa gtgaccttcc
     3061 tgccaattaa tcaggaatcc gcagccacta agcgttacct cgcgggggat attgatatca
     3121 ccgaatcctt cccgaaaaat atgtatcaga agctgttgaa ggatattccg gggcaggttt
     3181 atacgccgcc gcagctcggg acctattatt atgcgtttaa cacgcaaaaa gggccgacgg
     3241 cagatcagcg cgttcgtctg gcattaagta tgacgataga tcgccgcctg atgaccgaaa
     3301 aagtattagg gacgggcgaa aagccagcgt ggcattttac accagatgtt accgccggat
     3361 ttacgccgga accttcgccg tttgaacaaa tgagtcagga agaactgaat gcgcaggcaa
     3421 aaactttgtt gagcgcagct ggttatggtc cgcaaaaacc gctgaagctg acgcttttgt
     3481 ataacacttc agaaaaccat caaaaaattg cgattgctgt agcatcgatg tggaaaaaga
     3541 accttggcgt agatgttaaa ttgcaaaatc aggaatggaa aacctatatc gatagccgta
     3601 acaccggcaa ttttgatgtt atccgcgcct catgggtggg ggattataat gaaccctcca
     3661 ctttcctgac attattaacg tcaacgcatt caggaaatat ttcacgcttt aacaatccgg
     3721 catacgacaa agttctggcc caggcatcga cggaaaatac cgttaaagcg cgtaatgccg
     3781 attacaacgc ggcagaaaaa atcctcatgg agcaagcacc gattgcacca atttatcaat
     3841 ataccaatgg acgattaatc aagccgtggc tgaaaggtta tcccattaat aacccggaag
     3901 atgtggcgta cagccggact atgtatattg tgaagcattg atgtgatggg aactggcgtt
     3961 acccttgtgg ataacgccag tgatgtctga tttagcgccc ctgttccggc ggtgtaatgt
     4021 tatccatata cagcgtctgg ctggggaagg caaagtccgc gccgtgtgac tgcacaatat
     4081 cgataatctt caaataaacg tcttgctgtg cagcaagcca ttcagcccat accgtggttt
     4141 tggtaaagca ataaaccata atattcaatg acgagtcggc aaactggttg aaataaacca
     4201 gtaaggtttg tcgctggtcg atggccgggt gatttttcag catctcacgt acggcttcga
     4261 caataacgcc cacttttgcc gcatcctcat aacgtaaacc aatggtcgtg gtaatgcggc
     4321 ggttggtcat tcgtcctggg ttttctacgc tgatcgacga aaacagcgag ttcggtacgt
     4381 acaatggacg attatcaaag gtcgtaattt tggtaattcg ccagccaatt tccgctactg
     4441 taccttcgat atttctgtcc ggtgaacgga tccagtcgcc aatactgaaa ggacggtcga
     4501 aatagagcat gatcccggaa aagaaattac tcagaatatc tttaccggcc ataccgacag
     4561 ccagaccacc aataccacca aaggtcagca agccagaaag gctcatgccg aaatgttcgc
     4621 cataaagcag aacaagcacc acaataatgg tgattttgat aatgcgcgac ataatccgcg
     4681 cactggtgat atcgcgacct tttttaatct gctgtttttc aaactgatta atcagcaaaa
     4741 atagcttaat cgtcagaata accgcaatca gggacgtaca gataaaatcg ataacgtctg
     4801 gggtgataaa tttgagttta tagttttcta taacataatt aata
//
LOCUS       DAKISA010000193         4798 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.196, whole genome shotgun sequence.
ACCESSION   DAKISA010000193 DAKISA010000000
VERSION     DAKISA010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.196"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(74..517)
                     /locus_tag="OVW45_004217"
     CDS             complement(74..517)
                     /locus_tag="OVW45_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCV5528520.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEVLVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVEE"
     gene            674..1603
                     /gene="metA"
                     /locus_tag="OVW45_004218"
     CDS             674..1603
                     /gene="metA"
                     /locus_tag="OVW45_004218"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCV5528521.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            1872..3473
                     /gene="aceB"
                     /locus_tag="OVW45_004219"
                     /pseudo
     CDS             1872..3473
                     /gene="aceB"
                     /locus_tag="OVW45_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            3503..4794
                     /gene="aceA"
                     /locus_tag="OVW45_004220"
                     /pseudo
     CDS             3503..4794
                     /gene="aceA"
                     /locus_tag="OVW45_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
BASE COUNT         1196 a         1263 c         1217 g         1122 t
ORIGIN      
        1 ttggcgcaga aaaaaatgcc gggtgcgacg ctggcgcgtc ttctccagcc tgggcaggca
       61 cggcaggggc agcttattcc tccacatacg ccagattcag caacggatac ggtttcccca
      121 aatcgtccac ctcagagcgt cccgtaacct taaaacccac cttcttatag aacccaaccg
      181 cctgctcatt ttgctcatta acgttggttg tcagttccgg tgccatcgag agcgcatgct
      241 ccaccagcat ccgacccacg ccgcagccgc acacatcagg atcgataaac agcgcatcca
      301 tatgctgccc acttaacaac ataaacccaa ccggctgatc ccgttcatta accgcgaccc
      361 acaacggcgc ttccggcagg aaggaacgaa ctagcacctc cagctcggcc cgatactctg
      421 ctgataaaaa atcgtgtgtg gcatcgacag aacgacacca aatcgcaacg agttcctccc
      481 cttcctcatg ccgtgagcgg cgaatactaa taaccatttt ctctcctttt cgtcattctt
      541 atattctaac gtagcctttt ccttgaaact ttctcacctt caacatgcag gctcgacatt
      601 ggcaaatttt ctggttatct tcagctatct ggatgtctaa acgtataagc gtatgtagtg
      661 aggtaatcag gttatgccga ttcgtgtgcc ggacgagcta cccgccgtca atttcttgcg
      721 tgaagaaaac gtctttgtga tgacaacttc tcgtgcgtct ggtcaggaaa ttcgtccact
      781 taaggttctg atccttaacc tgatgccgaa gaagattgaa actgaaaatc agtttctgcg
      841 cctgctttca aactcacctt tgcaggtcga tattcagctg ttgcgcatcg attcccgtga
      901 atcgcgcaac acgcccgcag agcatctgaa caacttctac tgtaactttg aagatattca
      961 ggaacaaaac tttgacggtc tgatagttac cggcgcgccg ctgggcctgg tggagtttaa
     1021 tgatgtcgct tactggccgc agatcaaaca ggtgctggag tggtcgaaag atcacgtcac
     1081 ctcgacgctg tttgtctgct gggcggtaca ggccgcgctc aatatcctct acggcattcc
     1141 taagcaaact cgcaccgaca aactctctgg cgtttacgag catcatattc tccatcctca
     1201 tgcgcttctg acgcgtggct ttgatgattc attcctggca ccgcattcgc gctatgctga
     1261 ctttccggca gcgttgattc gtgattacac cgatctggaa attctggcag agacggaaga
     1321 aggggatgca tatctgtttg ccagtaaaga taagcgcatt gcctttgtga cgggccatcc
     1381 cgaatatgat gcgcaaacgc tggcgcagga atatttccgc gatgtggaag ccggactaga
     1441 cccggatgta ccgtataact atttcccgca caatgatccg caaaacaaac cgcgcgcgag
     1501 ctggcgtagt cacggtaatt tgctgtttac caactggctc aactattacg tctaccagat
     1561 cacgccatac gatctacggc acatgaatcc aacgctggat taatcttctg tgatagtcga
     1621 tcgttaagcg attcagcacc ttacctcagg caccttcggg tgcctttttt atttccgaaa
     1681 cgcacctcag caagtgaata aattttattc atattgttat caataagtta tcaggcattt
     1741 ttaattaaaa tggaaattgt ttttgatttt gcattttaaa tgagtagtct tagttgtgct
     1801 gaacgaaaag cgcacaacga tccttcgttc acagtgggga agttttcgga tccatgacga
     1861 ggagctgcac gatgactgaa caggcaacaa caaccgatga actggctttc acaaggccgt
     1921 atggcgagca ggagaagcaa attcttactg ccgaagcggt agaatttctg actgagctgg
     1981 tgacgcattt tacgccacaa cgcaataaac ttctggcagc gcgcattcag cagcagcaag
     2041 atattgataa cggaacgttg cctgatttta tttcggaaac agcttccatt cgcgatgctg
     2101 attggaaaat tcgcgggatt cctgcggact tagaagaccg ccgcgtagag ataactggcc
     2161 cggtagagcg caagatggtg atcaacgcgc tcaacgccaa tgtgaaagtc tttatggccg
     2221 atttcgaaga ttcactggcc ccggactgga acaaagtgat cgacgggcaa attaacctgc
     2281 gtgatgcagt taacggcacc atcagctata ccaatgaagc aggcaaaatt tatcagctca
     2341 agcccaatcc agcggttttg atttgtcggg tacgcggtct acacttgccg gaaaaacatg
     2401 tcacctggcg tggtgaggca atccccggta gcctgtttga ttttgcgctc tatttcttcc
     2461 acaactatca ggcactgttg gcaaagggca gcggtcccta tttctatctg ccgaaaaccc
     2521 agtcctggca ggaagcggcc tggtggagcg aagtcttcag ctatgcagaa gatcgcttta
     2581 atctgccgcg cggcgccatc aaggcgacgt tgctgattga aacgctgccc gccgtgttcc
     2641 agatggacga aatccttcac gcgctgcgcg accatattgt tggtctgaac tgcggtcgct
     2701 gggattacat cttcagctat atcaaaacgt tgaaaaacta tcccgatcgt gtcctgccag
     2761 acagacaggc cgtgacgatg gataaaccat tcctgaatgc ttactcacgc ctgctgatta
     2821 aaacctgcca taaacgcggt gcttttgcga tgggcggcat ggcagcgttt attccgagca
     2881 aagatgaaga gcgtaataac caggtgctca acaaagtaaa agcggataaa gcgctggaag
     2941 tcaataacgg tcacgatggc acatggatcg ctcacccagg ccttgcggac acggcaatgg
     3001 cggtattcaa cgacattctc ggctcccgta aaaatcagct tgaagtattg cgcgaacaag
     3061 acgcgccgat tactgccgat cagctgctgg caccttgtga cggtgaacgc accgaagaag
     3121 gtatgcgcgc caacattcgc gtggctgtgc agtacatcga agcgtggatc tccggcaacg
     3181 gctgcgtgcc gatttatggc ctgatggaag atgcggcgac ggctgaaatt tcccgtacct
     3241 cgatctggca gtggatccat catcaaaaaa cgttgagcaa tggcaaaccg gtgaccaaag
     3301 ccttgttccg ctagatgctg ggcgaagaga tgaaagtcat tgccagcgaa ctgggcgaag
     3361 aacgtttctc ccagggccgt tttgacgatg ccgcacgctt gatggaacag atcaccactt
     3421 ccgatgagtt aattgatttc ctgaccctgc caggctaccg cctgttagcg taaaccacca
     3481 cataactatg gagcatctgc acatgaaaac ccgtacacaa caaattgaag aattacagaa
     3541 agagtggact caaccgcgtt gggaaggcat tactcgccca tacagtgcgg aagatgtggt
     3601 gaaattacgc ggttcagtca atcctgaatg cacgctggcg caactgggcg cagcgaaaat
     3661 gtggcgtctg ctgcacggtg agtcgaaaaa aggctacatc aacagcctcg gtgcactgac
     3721 tggcggtcag gcgctgcaac aggcgaaagc gggtattgaa gcagtctatc tgtcgggatg
     3781 gcaggtagcg gcggacgcta acctggcggc cagcatgtat ccggatcagt cgctctatcc
     3841 ggcaaactcg gtgccagctg tggtggagcg gatcaacaac accttccgtc gtgccgatca
     3901 gatccaatgg tccgcgggca ttgagccggg cgatccgcgc tatgtcgatt acttcctgcc
     3961 gatcgttgcc gatgcggaag ccggttttgg cggtgtcctg aatgcctttg aactgatgaa
     4021 agcgatgatt gaagccggtg cagcggcagt tcacttcgaa gatcagctgg cgtcagtgaa
     4081 gaaatgcggt cacatgggcg gcaaagtttt ggtgccaact caagaagcta ttcagaaact
     4141 ggtcgcggcg cgtctggcag ctgacgtgac gggcgttcca accctactgg ttgctcgtac
     4201 cgatgccgat gcagcggatc tgatcacctc cgattgcgac ccgtatgaca gcgaatttat
     4261 taccggcgag cgtaccagtg aaggcttctt ccgtactcat gcgggcattg agcaagcgat
     4321 cagccgtggc ctggcgtatg cgccatatgc tgacctggtc tggtgtgaaa cctccacgcc
     4381 ggatctggaa ctggcgcgtc gctttgcaca agctatccac gcgaaatatc cgggcaaact
     4441 gctggcttat aactgctcgc cgtcgttcaa ctggcagaaa aacctcgacg acaaaactat
     4501 tgccagcttc cagcagcagc tgtcggatat gggctacaag ttccagttca tcaccctggc
     4561 aggtatccac agcatgtggt tcaacatgtt tgacctggca aacgcctatg cccagggcga
     4621 gggtatgaag cactacgttg agaaagtgca gcagccggaa tttgccgcta taccttcgta
     4681 tctcaccagc aggaagtggg tacaggttac ttcgataaag tgacgactat tattcagggc
     4741 ggcacgtctt cagtcaccgc gctgaccggc tccactgaag aatcgcagtt ctaagcaa
//
LOCUS       DAKISA010000194         4782 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.197, whole genome shotgun sequence.
ACCESSION   DAKISA010000194 DAKISA010000000
VERSION     DAKISA010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.197"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            8..83
                     /locus_tag="OVW45_004221"
     tRNA            8..83
                     /locus_tag="OVW45_004221"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41..43,aa:Thr,seq:ggt)
     gene            123..238
                     /gene="rrf"
                     /locus_tag="OVW45_004222"
     rRNA            123..238
                     /gene="rrf"
                     /locus_tag="OVW45_004222"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(301..1058)
                     /locus_tag="OVW45_004223"
                     /pseudo
     CDS             complement(301..1058)
                     /locus_tag="OVW45_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            complement(1066..2169)
                     /locus_tag="OVW45_004224"
     CDS             complement(1066..2169)
                     /locus_tag="OVW45_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCV5528522.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTERTPH"
     gene            complement(2179..3360)
                     /locus_tag="OVW45_004225"
                     /pseudo
     CDS             complement(2179..3360)
                     /locus_tag="OVW45_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3428..4453)
                     /locus_tag="OVW45_004226"
     CDS             complement(3428..4453)
                     /locus_tag="OVW45_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCV5528523.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTDYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
BASE COUNT         1310 a         1234 c         1143 g         1095 t
ORIGIN      
        1 ttagcgtgct gatatggctc agttggtaga gcgcaccctt ggtaagggtg aggtccccag
       61 ttcgactctg ggtatcagca ccacttttta ggttaaagtt cggcagatta gaaaagaatt
      121 tgtctggcgg cagtagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac
      181 gccgtagcgc cgatggtagt gtagggtctc cccatgcgag agtagggaac tgccagacat
      241 caaatcaagc gaaaggccat ccgaaaggat ggcctttttg cttttcgaac taacattcaa
      301 ttaatggatt acctgcgata aaaatgccct cgtacgctct gatttaggat gcgcaaaaaa
      361 ttcatcaggt gcagcttgct ccactatttc cccacgatcc ataaaaatca cccggtcagc
      421 gaggttcgcg caaaccccat ctcatgcgtt acacacaaca ttgtcatacc cgattgcgcc
      481 agcccaatca tcgtatccag cacctctttc accatctccg gatcgagcgc cgacgttggc
      541 tcatcaaaca acataatttt cggcttcata cacagcgaac gcgcaatggc aacgcgttgc
      601 tgctgaccac cagaaatttg tccgggaaac ttatgcgcat gttcggcaat tctcacccgc
      661 tcaaggtaat gcatcgccag agcttcagcc tctttctttg gcatcttgcg tacccaaatc
      721 ggtgccaggg tacagttctg tagaacggtc agatgaggga agagattgaa atgctgaaag
      781 accattccca cttcctgcct gacgcgctca atattgcgga tatcttcatt aagttcgatg
      841 ccatctacta cgatccgtcc ctgttgatgt tcttccagat gattaataca acgaatggtt
      901 gtcgatttac cggaaccaga agggccacac agaacgatcc gttctcccag ttgcacggtt
      961 aaatttatat ttttcaaaac atggaattgt ccataccatt tattgacgtt ttccagcgta
     1021 atcatcgcgt tagcaggttg cagtaaaatt tggctcatag tgtcctcaat gcggtgtacg
     1081 ctcggtgtta aaacgttttt ccagatgctg gctatagcgc gacatgctga aacagaagat
     1141 ccagtagatc agtgcggcga aaacataccc ttccgtcgac atacccaacc aggcgggatc
     1201 aacggttgcc tgctgaacgc tactgaaaag atcgaacaac ccgataatga tcaccaggct
     1261 ggtatctttg aagagtgcga tgatggtatt taccagccca ggaattacca gcttcaacgc
     1321 ctgtggcaga ataaccagcc cctgagtttt ccagtaaccc aatgccagtg actctgccgc
     1381 ttcatattgc cctttaggca gcgcctgtaa tccgcctcgc acgacttccg caacatatgc
     1441 tgactgaaac aggatcacgc caaccagcgc gcggatcagt ttgtcgatat tggtgccttc
     1501 tgccataaac aaaggcagca tgaccgaaga cataaacaat acggtaatca gcggtacgcc
     1561 gcgccagaac tcgataaaaa tgaccgataa gatacgcaca atcggcatat gggagcggcg
     1621 acctaacgcc agtaagatcc cccacggtag cgcccccgca atcccaacgg atgcaataat
     1681 taaagttagc gtcagcccgc cccattgccg ggtttcaacc cgctccagac caaaaaaacc
     1741 gccatacatc agccaccaga caatcagtgg gtaaatcacc gcccagaccg caatatagcg
     1801 accgcgatgc gggagtattt tccagaacat tggtgcgatt gacacaaggc caatcagtaa
     1861 tgccaggtta attcgccagc gttggtcgtg tgggtaaagt ccatacataa actgaccaaa
     1921 tcgttcgtgg atgaagaccc aacaggcacc ggcttttgtg cagtcggcac gcgttgagcc
     1981 aacccaatta gcctgcaaaa atgcccagtt cagcaacggt gggatcagtt cccacatcaa
     2041 ccatatgcag ccaatagtca gcaggctatt gctccagctg gagaacagat tttttcgcac
     2101 ccacaccatc gcgcggcttg agttatggct cgctgggcgc gagggctgag acagcaatac
     2161 ttttgtcatc atgactcctt agcgttcaac gatcgcgatg cggcggttat agatattcat
     2221 cagcagcgag atagtcaggc tgataatcag atagaccgac atggtcatgg ctatcgtctc
     2281 gatggcttgc cccgtctggt tcagcacggt gccagcaaac agcgaaacca tatcgggata
     2341 gccaatagcg gcggcaagag aggagttttt gacgatgttg agatactggc tggttaacgg
     2401 cggaataatc actcgcagtg cctggggaat aatgacctag cgtagcgtaa ccgggttggg
     2461 taatcccagt gaccgagccg cttcatgttg accataaggc actgcctgga tcccagcgcg
     2521 gataatctcg gcgataaatg cagatgtata aaccgaaagt gccagcgtta aggctgccag
     2581 ttcaggaatt aaaaccatcc cgccgcggaa gttaaagcct cgtagggctg ggacatccca
     2641 gtgtagtgct gcgccaaaaa gccattgcgc cagtaaaggc aaaccaatga tcaacactgc
     2701 ggcgatcggc caggtgcggc gcagttgtcc ggtctttatc tggtatgttt tattaaagcg
     2761 gaatagccca acagaaagga ctatagccat aacaacagcc aggataaacg caataaatcc
     2821 gtctcccagc tgcggtgacg gaatataaag cccacgattg ctcaaaaagg ccagatcaaa
     2881 cgcgctcaca gcctggcgtg gtccgggcaa attgcgcaac acggcaaagt accagaagaa
     2941 gatttgcagc agcgggggaa tattacggaa gatctcaata taaattgtgg aaagctttcg
     3001 tagcagccag ttatccgaaa gtctcgccag accgataaag aagcccaaaa cagaagcgaa
     3061 cacaatacac aatgcagaaa ccagtagcgt attgagtaag ccgacaataa aaacgcgtcc
     3121 gtaggtgtcg ccctgctggt aatcgatcaa atgctggaca ataccgaagc cagcgccgcg
     3181 atccagaaag gcaaaacctg aagtaatgcc acgattattg agattcgtta cagtgttgtg
     3241 aaataaccaa ccaacaatgc cgacaacagc aacaacggca aggatctgga ataaccaggc
     3301 acgaaccgta gggttggcaa aagagagtga gcctttaacg gttgagcggc gatgagacat
     3361 agaaacctcg gaaagcataa cgctgaagag agcgccgccg tagcgacgct cgttacctca
     3421 caccgactta acgcacgggc ggcgcgtact gaataccgcc gttattccag agattatttt
     3481 gcccacgttt aattttcagc gggctttctg aacctacgtt acgctcgaaa atttccgagt
     3541 agttacccac ctgtttgatg atgttatagg cccatttatt atccagcttc agatccttgc
     3601 cgtaatcgcc ttctttaccc agcagatgtg ccatatcagg cgttgctgga ttagccgctt
     3661 tttcatcgac gttctgggaa ttgatgccca tctcttcagc attcagcatg gcgaaaagcg
     3721 tccagcgtac aatcgagaac cattcatcat cgccacgacg aactaccggc ccaagcggtt
     3781 ctttagagat aacctccggt aagacaatcc attcagctgg gttgcttaat ttgatgcgca
     3841 gggcatacag ttgcgactga tccgaggcca gcgtatcgca gcgaccagat tccagtgcct
     3901 tcgctgattc gtcagagcga tcgaaagtca ccggtgtgta cttcatattg tttgccttga
     3961 aatagtcggc gacgttgagt tcggtatctg tacccgcctg aatacagacg gtagcgccat
     4021 ccagttcttt cgcgcttttt agccccgctt tatcgtgcgt caggaagcca atgccgtcgt
     4081 aatcagtgac gccagtaaat gccattccca tcccggcatc gcgagatgaa gtccaggtcg
     4141 tattacggga gagcaaatcc acctcccctg actgtaaagc ggtgaagcgt tcttttgcag
     4201 tgagcggggt atatttcact ttcgtgtcgt caccaaatac agcagcggca acaccacgac
     4261 aaacatctac atcaatacct gaaaacttac cgtctgcatc ggcataagag aacccaggta
     4321 atccatcact gatcccgcat tgcacaaaac cttttttctg tacggcatca agcgtcgcgc
     4381 cagcatgcgc ctgatttgca acggcaagca gcacgctggc ggcagccagt gtggctatca
     4441 tcatcttttt cataatgctt cctgtgaggc gaaaattatc gttataggac gtttgggaaa
     4501 cgtcttttac ctgtctgtgg ccatcctctt ataagcaaaa gtagtgccat cttttgcgca
     4561 cgatttcgcg tcgcaataag ctgagtgtag acaggaaata ataaaaacag cgccccgcaa
     4621 tggtgcgaaa atgaaacatg cgccacattt tggcgcaaaa tatttacgcg tgttttacta
     4681 agcagaataa tgattgttag cgtgagtata tttctggtgg gaggtgtgaa agttaaattg
     4741 atgcgttatt tcgaaagcag cgaaataacg cgatataaca tg
//
LOCUS       DAKISA010000195         4755 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.198, whole genome shotgun sequence.
ACCESSION   DAKISA010000195 DAKISA010000000
VERSION     DAKISA010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.198"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            77..577
                     /gene="ssb"
                     /locus_tag="OVW45_004227"
     CDS             77..577
                     /gene="ssb"
                     /locus_tag="OVW45_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCV5528524.1"
                     /translation="MGKDPEVRYIPNGGAVANLQVATSESWRDKQTGEMRELTEWHRV
                     VLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWDDDNGVTRYITEILVKNTGTMQMLGRT
                     AGAQTQPGEAQQFSGQPQPEPQAESGTKKRGAKAKGRGRKAAQPEPQPQPPEGEDYGF
                     SDDIPF"
     gene            634..864
                     /locus_tag="OVW45_004228"
                     /pseudo
     CDS             634..864
                     /locus_tag="OVW45_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
     gene            933..2891
                     /locus_tag="OVW45_004229"
     CDS             933..2891
                     /locus_tag="OVW45_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCV5528525.1"
                     /translation="MSVTESNAKTGHKSSRKPAKMQETALSALLAQTAEVSVPLASLI
                     KSPLNVRTVPYSVESVSELAESIKSVGLLQNLVVHALPGDRHGVAAGGRRLAALNVLA
                     ERGIFPADWPVRVKIIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPDVRVIRNLITESEVAVAGNTKYRFVGADAFSPDELRTDLFSDDGDGYVDRV
                     ALDAALLEKLQAVAEFLREAEGWGWCAGRMEPVGECREDARAYRSLPEPEAVLTEAEE
                     ERLNELMARYDALENQCEESDLLEAEMKLIDCMATVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDAAADADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMAMMAEKARL
                     AALLPEGWSRNMTTFMSLSQEVLLSLLSFCTACSIHGVQTREYGHTSRSPLDSLETAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNDAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMGSPRPQTDATERADNLADAA"
     gene            2946..3380
                     /gene="psiB"
                     /locus_tag="OVW45_004230"
     CDS             2946..3380
                     /gene="psiB"
                     /locus_tag="OVW45_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HCV5528526.1"
                     /translation="MKTELTLNVLQTMNAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGSFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDTQGYSVNDIIHILMAEGGQV"
     gene            3377..4096
                     /locus_tag="OVW45_004231"
     CDS             3377..4096
                     /locus_tag="OVW45_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HCV5528527.1"
                     /translation="MSVRSQALVPLSTEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWHARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEASLAAGE
                     AGHAVREMERWMVPNKLREAA"
     gene            4371..4532
                     /locus_tag="OVW45_004232"
     CDS             4371..4532
                     /locus_tag="OVW45_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCV5528528.1"
                     /translation="MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTA
                     TMAYESGGK"
BASE COUNT         1057 a         1257 c         1508 g          933 t
ORIGIN      
        1 acggtgatct aactcaagat aagcaggaca tattcgccat ttatcttgaa agaccacacg
       61 taccctaccc taccgtctgg gaaaagatcc ggaagtccgt tacatcccca acgggggcgc
      121 agtggcaaac ctgcaggtgg ccacgtcaga aagctggcgt gacaaacaga cgggggaaat
      181 gcgggagctg acggaatggc atcgcgtggt gctgttcggc aagctcgcgg aagtggcagg
      241 tgaatatctg cgcaagggcg cgcaggtcta catcgagggg cagcttcgca cccgtagctg
      301 ggacgacgac aacggcgtca cccgttacat cactgaaatc cttgttaaaa acacgggcac
      361 catgcagatg ctgggacgta ccgcaggtgc tcagactcag ccgggagagg cgcaacagtt
      421 cagcggtcag cctcagccgg aaccacaggc ggagtccggt acgaaaaaaa gaggcgcgaa
      481 agcgaaaggc cgtggacgta aggccgcgca gccggagcct cagccgcaac cgccagaggg
      541 tgaggattac gggttttcag acgatatccc gttctgaacg actgactgtg acaaccgccc
      601 cgccctgtgc ggggcatcac cggagagatg aggatgagcg aatatttcag aatacttcag
      661 gggctgcctg acggctcctt tacccgcaaa catgccgaag ccgttgccgt acagtaccgg
      721 aacgtcttta tcgaggatga ccacggggaa cattttcgcc tggttgtccg taataacggt
      781 gcaatggtct ggcgtacctg gaattttgag gacgatgccg ggtactggat gaaccatgcc
      841 atccgggatt tcggcattct taagtcagag aggtgccgga cgggaacgcg tccggcagac
      901 aatcattatt accgttttgg gaggtaataa ctatgtcagt tacagagtct aatgcaaaaa
      961 cggggcataa atccagccgt aaacctgcaa aaatgcagga aactgccctg tctgccctgc
     1021 tggcgcagac ggcggaagtg agcgtgccgc tggcctcgct gattaagtcg ccgctgaatg
     1081 tgcgcacggt gccgtattct gtggagtccg tcagcgaact ggcggagtcc attaagagcg
     1141 ttggcctgct gcagaatctg gtcgtgcatg ccctgccagg tgaccgccac ggtgtcgccg
     1201 caggtggtcg cagactggca gcactcaacg tgctggcaga gcgtggcatc tttccggctg
     1261 actggcctgt ccgcgtgaaa attattccgc aggaactggc gacggctgca tcgatgaccg
     1321 agaacggtca tcgtcgggat atgcaccctg ccgaacagat tgccggattc cgcgcaatgg
     1381 cgcaggaagg caaaacacct gcacaaatcg gtgatttgct gggctattca ccccgccacg
     1441 ttcagcgaat gctgaaactg gcagaccttg cgcctgtcat cctcgatgcg ctggcagaag
     1501 accgcatcac caccgaacac tgtcaggcgc tggcactgga gaacgacacc gcgcgtcagg
     1561 tgcaggtgtt tgaagccgcc tgtcagtcgg gatggggcgg taaaccggat gtgcgggtta
     1621 tccgcaacct gattacggaa agtgaagtgg cggtggcggg gaacactaaa taccgcttcg
     1681 tgggggctga tgccttctcg ccagatgaac tgcgcaccga tttgttcagc gatgacgggg
     1741 acggttatgt cgaccgcgtg gcgctcgatg ctgccctgct ggaaaaactc caggccgtcg
     1801 cagagttcct tcgggaagcc gaaggctggg gatggtgcgc cggacgcatg gaacctgtcg
     1861 gtgagtgccg tgaagatgcc cgggcatacc gcagtctgcc ggagccggaa gcggtgctga
     1921 cggaggcgga agaagaacgc ctgaacgaac tgatggcacg ttacgatgcg ctggaaaacc
     1981 agtgtgagga atccgacctg ctggaagccg aaatgaagct gattgactgc atggcgacgg
     2041 tcagagcgtg gacgccggag atgcgtgccg gaagtggcgt ggtggtgtcc tggcgttatg
     2101 gcaacgtatg tgtccagcgt ggtgtgcagt tgcgcagcga ggatgacgcg gctgccgacg
     2161 ctgaccgcac ggaacaggtg caggagaaag cgtcagtgga ggaaatcagt ctgccactgc
     2221 tgacgaaaat gtcttcagag cgcacgctgg cagtccaggc ggcgctgatg caacagccgg
     2281 acaaatctct ggcactgctg gcatggacgc tctgtctgaa tgtgtttggc agcggagcgt
     2341 acagtaaacc agcacaaatc agcctggaat gtaaacatta ttcgctgacc agcgatgcgc
     2401 catcggggaa ggaaggtgcc gcattcatgg cgatgatggc agaaaaagcc cgtcttgctg
     2461 ccctgctgcc ggaggggtgg tcacggaaca tgacgacgtt catgtcactc agtcaggagg
     2521 tgctgttatc cctgctcagt ttctgcaccg catgcagcat ccacggtgtt cagacccgtg
     2581 agtatggtca cacgtcacgc agtccgcttg actcgctgga aaccgctatc ggctttcaca
     2641 tgcgcgactg gtggcagccg acgaaagcaa acttcttcgg acacctgaaa aagccgcaga
     2701 ttatcgcagc cctgaatgat gccggactgt ccggtgccgc acgggacgcg gagaagatga
     2761 agaaaggcga tgccgctgaa catgcagagt tccacatgaa agacaaccgc tgggtgcctg
     2821 gctggatggg ttcaccacgt ccacagacgg atgccactga acgcgccgat aacctggctg
     2881 atgccgcctg atgaacaacc acaccgcccc gccggagacg gggcggcagc aagggagata
     2941 ccgtgatgaa aactgaactg accctgaatg tattacagac catgaacgca caggaatatg
     3001 aagatatccg ggctgccgga agtgatgaac gccgtgagct gactcacgcc gtgatgcggg
     3061 agctggatgc gccggataac tggacgatga acggcgagta cggcagcgag tttggcagct
     3121 ttttccccgt ccaggtccgt ttcacgcctg cccacgaacg ttttcacctg gcgttatgtt
     3181 caccgggcga tgtctctcag gtctgggtgc tggttctggt gaatgccggt ggtgagccgt
     3241 ttgcggtggt tcaggtacag cggcggtttg caccggaagc cgtcagccat tcactggcac
     3301 tggcggcgtc actggatacg caggggtaca gcgttaacga catcatccat atcctgatgg
     3361 cggaaggagg tcaggtatga gcgtacgttc acaggcactg gttcccctca gcacagagca
     3421 acaggccgca tggcgggcgg tggcggagac agaaaaacgc cgccatcagg gtaacacgct
     3481 ggcggaatat ccgtatgcgg gggcgttttt ccgctgtctg aacggaagcc gcaggatatc
     3541 gctgtctgac ctgcgatttt tcatgccgtc cctgaccgca gaagaactgc acggcaaccg
     3601 tcttcagtgg ctgtatgcca tcgatgtgct gattgaaaca cagggcgagg tctgcctcct
     3661 tccccttcct ggtgatgcag cagagcggtt gtttccgtcg gtgcggtttc gtgtcaggga
     3721 gcgcagtcgc cataaatccg cgctggtcat gcagaagtac agccgccagc aggcacggga
     3781 ggcagagcag aaagcccgtg cgtaccaggc gctggtcgct caggctgaaa tcgaactggc
     3841 attccattcg ccggaaacag tcggaagctg gcatgcacgc tggagtgacc gggtggcaga
     3901 gcatgacctg gaaaccctgt tctggcagtg gggcgagcgt tttccttcac tggccggaat
     3961 ggaacgctgg cagtggcagg atatgccatt ctggcaggtt attgcggaag ccagtcttgc
     4021 agcaggagag gccggtcacg ctgtccgtga gatggagcgg tggatggtgc cgaacaaact
     4081 ccgggaggca gcgtgatgcg gcaacaatca cacggatttc ccgtgaacgg tctgaatgag
     4141 cggattcttt tcaggaaaag tgagtgtggt cagcgcgcag ggatatgggc tatgatgtgc
     4201 ccggcgcttg aggctttctg cctcatgacg tgaaggtggt ttgttgccgt gttgtgtggc
     4261 agaaagcaga aagccccgta gttaattttc attaacccac gaggcccact gtatgtctga
     4321 tccacgtcag tatggcctct taccgtgcaa attgcaagga ggagtaaacc atgaaactac
     4381 cgggaaacgc ccttatctgg tgcgtattaa tcgtgtgctg cacgttgttg atattcacac
     4441 tcctgacccg gaatcgcctg tgcgaagtcc ggctgaagga cggatacagg gaagttacgg
     4501 caacaatggc ttacgaatcc ggcggtaagt agcaacctgg aggcgggcgc aggcccgcct
     4561 tttcagggct gatgctggtc tgactgcact gaagcgcctt tataaagggg ctgctggttc
     4621 gccggtggcc cctttctcct tgctggcatt gtgcgggcat gaacaaccag gctgccagga
     4681 aggccgtttc ccttcgggaa acggggaaga acgtgcgcct gcggcgcccg ttcttttccc
     4741 ccaccttctc tgttt
//
LOCUS       DAKISA010000196         4701 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.199, whole genome shotgun sequence.
ACCESSION   DAKISA010000196 DAKISA010000000
VERSION     DAKISA010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.199"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            387..1469
                     /locus_tag="OVW45_004233"
     CDS             387..1469
                     /locus_tag="OVW45_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HCV5528529.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGMVYQF"
     gene            complement(1658..2041)
                     /gene="quuD"
                     /locus_tag="OVW45_004234"
     CDS             complement(1658..2041)
                     /gene="quuD"
                     /locus_tag="OVW45_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HCV5528530.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(2127..2267)
                     /locus_tag="OVW45_004235"
     CDS             complement(2127..2267)
                     /locus_tag="OVW45_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HCV5528531.1"
                     /translation="MMFEFYMAELLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(2264..2626)
                     /gene="rusA"
                     /locus_tag="OVW45_004236"
     CDS             complement(2264..2626)
                     /gene="rusA"
                     /locus_tag="OVW45_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HCV5528532.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVTRIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            complement(2623..2913)
                     /locus_tag="OVW45_004237"
     CDS             complement(2623..2913)
                     /locus_tag="OVW45_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCV5528533.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTYFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(2906..3076)
                     /locus_tag="OVW45_004238"
     CDS             complement(2906..3076)
                     /locus_tag="OVW45_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528534.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(3076..3531)
                     /locus_tag="OVW45_004239"
     CDS             complement(3076..3531)
                     /locus_tag="OVW45_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcN family protein"
                     /protein_id="HCV5528535.1"
                     /translation="MTLPVDGIKLHRGNFAAIGQQIQPLLDAGQCLRLQVKPWREKRS
                     LSQNALSHMWYTEISEYLIARGKTFATPEWVKDAMKHTYLGYESKDRVDVVSGEVTTV
                     QSLRHTSELETGEMYIFLCKVEAWAMNIGCHLTIPQSCEYQQLRDKQEA"
     gene            3796..4173
                     /locus_tag="OVW45_004240"
     CDS             3796..4173
                     /locus_tag="OVW45_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528536.1"
                     /translation="MTNKENKDKYINKFKKELSKYIQPTAGVDIQLFNSKDGGGVIKA
                     TLNRSGKRKSSIAGNFTKLGEAITSSGQRVFGGDLSNVNFYGTNTIFDGDTIFLIKSP
                     DSEEWSSQKASKDVEGIVFGGKK"
     gene            4170..4598
                     /locus_tag="OVW45_004241"
     CDS             4170..4598
                     /locus_tag="OVW45_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528537.1"
                     /translation="MINITTKDGTQVSGEVRDLVILLETDGKTVSDYLQPIPPKKDTH
                     WSMIFIPVAIYILLNLVYIIAHPLFPEKYLKTSTSIFLFISFILSAIVAYFVFHKHKS
                     ATLSICSLVFLVLIVGANVGFISYSDLTQKATQKLDSLSK"
BASE COUNT         1350 a         1049 c          980 g         1322 t
ORIGIN      
        1 ttttttacgt acgtaaacca ctaaatgata tttgcgagaa tgctaccgag tattgaaaac
       61 accactacaa atacataagc aaatcccaac aaataaccaa caaataattt ccagtgttat
      121 ttttagccgg tttaaattga accttcaaat tatagagcac ttataaataa cagccgttaa
      181 tataaattgg ctaatagatt tatttttatt cagccaagag ccatgaatag gattcgatag
      241 aaaaaagttc agataaaaat agagatctac ttcacaaatc aaacgagaaa ccaaaactta
      301 catcttgaaa taatcacatt gattagatga atatttatcg cgcagtgaca tcatttttta
      361 ataatagttc aaaaaaaggg ctcacgatga aaaaattaac agtggcaatt tctgctgtag
      421 ctgcatcagt actgatggcg atgtctgctc aggcagctga aatttataat aaagacagta
      481 acaagctgga tctgtacggg aaagttaatg ctaagcacta cttctcctct aatgatgcag
      541 atgatggtga tactacttat gcccgtcttg gcttcaaagg tgaaacccaa atcaacgatc
      601 aactgactgg tttcggtcag tgggaatatg aattcaaagg caaccgcgct gaatctcaag
      661 gttcctccaa agataaaacc cgtcttgcct tcgctggcct gaaattcggt gactacggct
      721 ccatcgatta cggccgtaac tacggtgtag catacgacat cggtgcgtgg actgacgtcc
      781 tgccagaatt cggtggtgac acttggactc aaacagatgt gttcatgact ggtcgcacca
      841 ctggtgttgc aacttatcgt aacaacgact tctttggtct ggttgatggt ctgaactttg
      901 ctgctcagta tcagggtaaa aatgaccgca ctgacgtaac tgaagctaat ggtgatggtt
      961 tcggtttctc cactacttat gagtatgaag gattcggtgt aggtgcaacc tatgctaaat
     1021 ctgaccgcac taataatcag gttatctacg gtaacaacag cctgaatgca tctggtcaaa
     1081 atgctgaagt atgggcagct ggtctgaaat atgatgcgaa caacatctat ctggctacca
     1141 cctattctga aacccagaac atgactgttt ttggtaataa ccatattgcc aacaaagcac
     1201 aaaacttcga agtagttgca caatatcagt tcgacttcgg tctgcgtccg tccgttgctt
     1261 acctgcaatc taaaggaaaa gacttgggtg cgtggggtga tcaggacctg gttgaatata
     1321 ttgatgtagg tgcaacctat tacttcaaca aaaatatgtc cacttttgtt gattacaaaa
     1381 tcaacctgat tgataagagc gatttcacga aagcatctgg cgttgctacc gatgatatcg
     1441 ttgctgtagg tatggtttac cagttctaat ttgattacta aaagatatgt tgcgggaggc
     1501 tttgcctccc caacatataa gtggctccct caagccactt cctttaggag cacaaccttg
     1561 cttctaacta tataaacctt ctgttatata ttacccttta tttttggggg cgttgcaacg
     1621 ccccattttt aataattttt agtaaacaat tggcatatta attagagtta ttaacaacga
     1681 tatccatctc taaccggata tctaatgcca ttaacatccc ttcaattatg ccctcagcct
     1741 tctgtaacct tttcccgata taaccatcag agcagcaatg cttacctgcc agtgacatga
     1801 atgtcatacc gactacataa taatctacta ataaatcgtg caaatcgctg ttgttctttt
     1861 tcagacgggc catgcacccg caaataatca tcgcgtcatc gtcacaacat tgcgggcgag
     1921 attttacttt tgaagtaatt aatcccttaa aaccggcggc aatggacgac caggtcacat
     1981 cttcatgatt attagccgcc cacgctcccc aacgctcaag aaccatctga atatcacgca
     2041 tcaacttact ccacaaaaat cagaccagaa cgccaattac aagcaaaaat caacaaaaca
     2101 gtattagttg attgttatct ctgacttcat actcctgctc ctgtcagggt tttggcgtaa
     2161 ttcttcagta ttcggtaatc ggtcaaaaca gaaccgggga aacgatataa gcgcagatgc
     2221 ccccagcggt ggcgaagaag ttctgccata taaaactcaa acatcattca ttccccattt
     2281 cggtgatggt cagttccagc ctcccacctt tggtaacagg catcttcaca acgcggtaat
     2341 caacgacctg agcatcatcc agccagaaac ctgctttggt gagtgcgtca aaagcggctt
     2401 tttgcagatt atccaggtca cggcgacggc gatccggcat gtggcactca atacggattt
     2461 tcactggcat agccaggccg atatccagca ttgcgttttt aatgattcgg gtgacgttat
     2521 cgcggtatgc ctgcccttct gcgctgatgt gtgtgcgccc gcgattatgg cggtaatagc
     2581 ggttattgct cggaggccag ggtaatgtga tgctgtaggt attcacgcct taataacccc
     2641 ctctttcagc cagataacct gcgttctcgc cataccttcc agcgcgcatt cttttgcata
     2701 ctcagcatcg acaaaatatg tgcggcggtc gatttcgtcg tgacaggcag aacatgcaat
     2761 ggtggcaatc aggtctggcg gtttgatacc ggtaccgcac aatccagcca gccggatatg
     2821 tgccagtact gacgtttcag gattgccatt acatacgcca gggattctta cctggcattc
     2881 ccgaccacgc gctgcttttc tcaaatcagc catgattcct ccttgctgcc agtcgcaacc
     2941 attttttatc aaccaggcta gcggtatatc cgagcagtgt tggtatttcg gatggcttca
     3001 gctcaggctt acgcttacga cgatttgata ctctgtagat gtgcccgttc atgacacgaa
     3061 taagcggtgt agccattacg cctcctgctt atcgcgcagc tgctggtatt cacagctctg
     3121 cggaattgtc aggtggcagc cgatattcat cgcccaggct tcgactttgc acaggaagat
     3181 gtacatctcg ccggtttcca gctctgacgt atggcggagg gattggacgg tggtgacctc
     3241 gccggacacg acatctacac ggtctttgct ttcgtagccg agataggtgt gcttcatcgc
     3301 gtctttgacc cactcaggcg tagcgaaggt cttgccgcgg gcgatgagat attcgctgat
     3361 ctccgtgtac cacatgtggc tgagcgcgtt ctgcgacagg ctgcgcttct cgcgccacgg
     3421 cttaacctgc aggcgtaagc attgcccggc atccagcaat ggctgaatct gctggccgat
     3481 ggccgcgaag ttaccgcgat ggagtttgat gccgtctact ggcagagtca tacggcctcc
     3541 ctaacggaaa ccgcagaatg cagaaaatcg caggtgcatt tctgcatctg tgacaaggtg
     3601 atgagttcag attgtggtcg catttaagtc cccttaaatg cgcagaagtc accggagttg
     3661 ttcaggctcc gatgagagga ttatggcgga ttgattacga taaatcaaaa ctatcttgaa
     3721 gccaaaacag tgatcttcgc tttaaaaacc ttgtatactt tcacctaatc acagccagaa
     3781 ctatggatct aaccaatgac caacaaagaa aacaaagata aatatattaa caagttcaag
     3841 aaagagcttt caaaatatat acaaccaact gctggagtgg acattcaatt atttaactct
     3901 aaggatggtg gtggtgtaat taaagccact ttgaatagaa gtggaaaaag aaaatcatct
     3961 attgctggta attttactaa attaggtgag gcaattacct cgagtgggca gagggttttt
     4021 ggtggtgatc tatcaaatgt gaatttttac ggaacaaata cgatttttga tggagataca
     4081 atatttttaa ttaaatcacc tgattcagag gagtggtcat cacaaaaagc ttccaaagac
     4141 gtagagggca tcgtatttgg agggaaaaaa tgattaacat tactactaag gatggcactc
     4201 aagtcagtgg tgaagtaaga gatctagtaa ttcttcttga aactgatgga aaaacggtgt
     4261 ctgattattt gcagccaatc cctcccaaaa aagatacaca ctggagcatg attttcattc
     4321 cggtagcaat ttatatacta cttaatcttg tatacattat tgctcaccca ttatttcctg
     4381 aaaaatatct aaaaacatca acatctatat ttctattcat ttccttcata ttaagtgcaa
     4441 tagtagcata ttttgttttc cataagcata aatctgccac tctatccata tgcagcttag
     4501 tgttcttagt gttaattgta ggtgctaacg tagggtttat cagttacagc gacctaacac
     4561 aaaaagcgac gcaaaaatta gatagtttgt ctaaataatt atgttgccat gcgaagtatg
     4621 catggctgca tgtctgcctt ccatttaaaa tgggctaaga cctataaccc taaatattat
     4681 tctttattat cttctttacc a
//
LOCUS       DAKISA010000197         4695 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.200, whole genome shotgun sequence.
ACCESSION   DAKISA010000197 DAKISA010000000
VERSION     DAKISA010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.200"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(209..3313)
                     /gene="acrF"
                     /locus_tag="OVW45_004242"
                     /pseudo
     CDS             complement(209..3313)
                     /gene="acrF"
                     /locus_tag="OVW45_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(3325..4482)
                     /gene="acrE"
                     /locus_tag="OVW45_004243"
     CDS             complement(3325..4482)
                     /gene="acrE"
                     /locus_tag="OVW45_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HCV5528538.1"
                     /translation="MTKHARFFLLPSFILISAALIVGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDECTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT         1200 a         1205 c         1139 g         1151 t
ORIGIN      
        1 tcttattaac gagctgaatg ccattttatc accggaggtt ataaatattg cggaatgaat
       61 aatgacaaat tatgtgaatt gtaaaaatag tgaagggagc gggaactata gaaagttttt
      121 aaagagtgag tagaagaata tattattaat ttttatgggt ttcactgaaa ataataaagg
      181 cacccgaaag tgcctttatg tttctgattt atcctttaaa gcaacggcgg atcaccacaa
      241 agaacaccgg tacgaagaag attgccagca acgttgcaga gaccattcct cccattaccc
      301 cgatacccac tgcgttctgc gcgccactgc cggcaccgtt actgatagct agcggtaata
      361 cgccgagaat aaaggcgaga gaggtcatca ggataggacg cagacgcata cgtactgcca
      421 tcagtgtcgc ttcaacaaca cctttaccct ttttctccat gagatcttta gcgaactcaa
      481 caatcaaaat agcgtttttg gccgacaagc caattgtcgt tagcaagccc accataaagt
      541 agacgtcatt tttttgatta aagagtgttg ccgccagtag cacgccgaca atccctaacg
      601 gcacgaccaa cataaccgag acaggaattg accagctttc atagagtgca gcaaggcaca
      661 ggaagacaac cacaaaggaa attgctacca gagcgggagc ctggtttccc gataagcgtt
      721 cctgatacga catacctgtc cagtcataac caatgcccgc aggtaatttt gacgcaaggt
      781 tttccatcaa tgccatggca tcgccggaac tggttcctgg cgcggcttcc ccctgaatct
      841 ccattgacgg cagaccgttg tagcgttcca gtcgcggaga gccatacacc caatgtgaag
      901 tggtaaaggc agagaatggc accatttcgc cgttggcgct gcggacataa agtttatcga
      961 catcttctgg cagcatacgg aatttggcat ccgcctgaac atacaccttt ttcacgcgac
     1021 cacggtcgat gaagtcgtta acgtaagtcc cacccagcgc cgttgaaatg gtctgattga
     1081 tgtcagaaag tgaaacacct aatgcctgcg ccttttcctg gtcaacttcc agtttaaact
     1141 gcgcggtgtc ttccagacca ttaggacgca cgctgactaa gctggcagga tgttgcgccg
     1201 ccatgccaag caactggtta cgggcctggg tcagggcatc gtgacccagc ccagcctgat
     1261 caattaactc aaagtcgaaa cccgttgccg tgcccagttc aacaatggct ggcatattga
     1321 atggaatgac aaaaccgtcg cggatcttgc ccaattccat tttggcacga tggattaccg
     1381 cttccgcact gttttcgtca ccactacgct cttcccacgg tttcagactg acgaaagcca
     1441 taccggcgtt ttgtgcctgg ccgctgaagc taaagccgtt aaccgtaaag acactttcta
     1501 cgttcgcttt ctcgttattc agatagtaat ccgtcacttg atccaacact ttttgcgtcc
     1561 gctcttgcgt cgcgccagcg ggtaactgaa tcatggtcag aaagacaccc tgatcctctt
     1621 caggtaagaa ggaagacgga agacgtaaaa acaacaccac cattcctgca acaatcagcg
     1681 catagatcag taaatatcgt cctgtggaac cgaggatttt gccgacgctg ttggtgtagt
     1741 ggttaacgct atgatcgaag gtggtattaa accagccgaa gaaaccgccc ttattttcgt
     1801 gatgctcagc agagacgggt ttaagcagcg ttgcacataa cgcaggggta agaatcaatg
     1861 ccaccagaac agaaagcgcc attgccgaaa cgatggtgat agagaactgg cgataaattg
     1921 ccccagtaga accgccgaag aatgccatcg gaataaatac cgctgacagc accatcgcga
     1981 tacccaccag tgcgccctga atttgcgaca tcgatttttc cgtcgcttct tttggcggga
     2041 gcttatcctc catcatcacg cgctcgacgt tctccaccac cactatcgca tcatcgacga
     2101 gcagcccgat ggcaagcacc atcccgaaca tcgttagtgt gttgatggag taaccaaaag
     2161 cggcgaggat ggcaaacgtc cctaacaaca ccacgggtac cgcaatggtg gggatcagcg
     2221 ttgctcgcat attctgcaag aacagataca tcaccaggaa caccagcata atggcttcga
     2281 acagcgtttt taccacttcg tgaatagaaa gctggacgaa tggcgtggtg tcataagggt
     2341 agagaacctt cattccctgc gggaagaatg gctgtaattc cgccagtttt gccttaatgg
     2401 ctttcgcggt atcgagagcg ttcgcgccgg ttgctagctt aatccccagg cccgccgccg
     2461 gttttccgtt gatacgagcg ataacgttat agttttcacc gccaagttca actcgtgcga
     2521 catctttcag gcgtaccacc gagccgtcac tgtttacgcg cagggttact ttgccgaatt
     2581 cttccggatt ttttaaccgc gtctgagcaa taatcgaggc gttcaactgt tgccctggta
     2641 acgctggcgt tccgcccaac tgcccggcag cgatctgatc gttctgtacc ttcaactggt
     2701 taatcacatc aaccggtgtc agtttatatt tgtttagcag atcggcgtcc agccagatac
     2761 gcatcgcata ctgtgcgccg aaaagctgta cgtcaccgac gccattcaga cggctaagcg
     2821 tatctttaac gttagaggcc acatagtccg agatatcgtc ctgtgtggtg tctgggttat
     2881 cagagacaaa gcccgccacc atcaaatagc tgctactgga cttttcaaca ctgatcccct
     2941 gctgctgaac ctcctgcggc agcaacggcg tggcgagctg gagtttgttc tgcacctgca
     3001 cttgcgcgat atcaggatcg gtcccggact ggaaggtaag ggtaattgtc acgctaccgg
     3061 cggaatcgct ggtggaggac atatacatca ggttatcgat accattcata ttctgttcga
     3121 taacctgcgt caccgtatcc tgcacggtct gcgcatcagc acctggatag tttgcctaaa
     3181 cagaaaccgc aggcggtgca atggttggat actgagcgac gggcaattgt aggatcgcca
     3241 gtgcgcccgc catcatcaga ataatggcca gcacccatgc aaatatcggt cgtcgaataa
     3301 aaaagtttgc catgtcagat taccttactt cgatgcagta tctgcggggg tatcggtagt
     3361 ggctttaacc tgctctcccg gacgcgcttt ttgcaggccg ctgacaatga cttgatcgcc
     3421 agatttcaga ccttcgctaa tcaaccattt atcgccaatc gcctgactgg caacgacagg
     3481 gcgcgcttcc acctgacttt tatcgttaac aatcagcacg gttgcatcac cacgcggtgt
     3541 gcggctaacg ccttgttgcg ggataagaat ggcgtcaggt tggacgcctt catcaatccg
     3601 tgcacgcaca aacatacccg gcaaaagcgt atgttgcggg ttagggaaga cagcacgtag
     3661 ggttatggag ccggtgcatt catcaacggt cacatcagag aattgcagcg tacctttcag
     3721 gggataggtt tgaccgtttt ccatgaccaa ctctacgttg ctggtggcgt tttccttatg
     3781 caaatttcct tgctctacgg attgcttcag cctcataaaa tcgttgctgg attgggtcac
     3841 atcaacgtag ataggatcga gctgctggac agtcgccagt tcagtcgttt gcccattagt
     3901 gacaagagcg ccttcggtca cggtcgattt gccgatacgt ccgctaattg gcgcagtcac
     3961 tttggtataa gcgagattga tgcgagcgct ttcgactgtg gcttttgcgg caatcacggc
     4021 ggcatcggcc tggcgagcat cagcaatggc ctggtcgtac tcctgctggc tgatgtattt
     4081 cgtacccacg agcggaacgt aacgttttac cgtcaaatgc gcgatggcgg cggcggcttc
     4141 acttttcgcc agttcgcctt tcgcgctgtc ataatttgcc tgataggtcg cgggatcgat
     4201 ctggtacagg gactggcctg cttgtacatc gctgccttca gtgaaattac gattcagtac
     4261 gatcccgcta acctgtgggc gaacttcggc tatacggtaa gcattggtgc ggcctggtaa
     4321 ttcagtctta acttctaacg gggccgtttt cacaatatga acggtaacct gcggttcacc
     4381 gacgtgagct ttctcttctc ccttatcgtt acaaccgacg attaaagccg cggagatcag
     4441 aataaaggag ggcaggagga aaaacctggc atgtttcgtc attactattc ctcaaaaaaa
     4501 caaaagcgcg ttatttaccc agaaaggcaa cgcgttaact cgcagaaaga aaaatacagt
     4561 ttgctatcct acaaattatc attcgtcgat gtaaggaata gttatgaata ctggcgtctc
     4621 aaggcacata aacacaaaaa aagattaata ttctactgtt ttattttgac gcgggttgaa
     4681 agaggcagaa ttaaa
//
LOCUS       DAKISA010000198         4621 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.201, whole genome shotgun sequence.
ACCESSION   DAKISA010000198 DAKISA010000000
VERSION     DAKISA010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.201"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..1361)
                     /locus_tag="OVW45_004244"
     CDS             complement(<1..1361)
                     /locus_tag="OVW45_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCV5528539.1"
                     /translation="MNRTSPHYCRRSVLSLLISALIYAPPVMAAFTTNVIGVVNDETV
                     DGNQRVDERGTTNNTHIINHGQQSVYGGVSNGSLIESGGYQDVGRNNNYMGQSNNTTI
                     NGGRQTIHDGGISTGTIIDSGNQDVYTGGISNGTTIKGGNSHISGGTANGTIIDGGGQ
                     TVTTQGHVDGTTINKSGYQDITQGSMATNTIINGGRQYVEQSTVGTTTIKNGGEQRVY
                     ESHALDTTIEGGTQSLNNKSTAKNTQIYSGGTQIVDYTSSSDVIEVYSGGVLDVSGGT
                     ATNVTQHDGAILKTNTNGTTVSGTNSEGAFSIHNHVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVTRNATNTIINGGTQNINNHGIATGTNIN
                     SGTQNIKSGGKADTTNISTGSRQVVEKDGTATGSNISAGGSLIVYTGGIAHGVNQETG
                     SALV"
     gene            complement(1426..1545)
                     /locus_tag="OVW45_004245"
     CDS             complement(1426..1545)
                     /locus_tag="OVW45_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCV5528540.1"
                     /translation="MPLSYVEHHHFIMMSLMSGEHNTLALSLQDEPPGLVPAG"
     gene            complement(1700..1918)
                     /locus_tag="OVW45_004246"
     CDS             complement(1700..1918)
                     /locus_tag="OVW45_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCV5528541.1"
                     /translation="MKKLLRTLSAVDARVVCKPHRLGRSMWHLVVLLEELCKRGINFR
                     ALAQSIFAQQWGDECCKSKRICDLKVIV"
     gene            complement(2272..3750)
                     /locus_tag="OVW45_004247"
     CDS             complement(2272..3750)
                     /locus_tag="OVW45_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HCV5528542.1"
                     /translation="MLVSKSNEFNTSSFIDSGNCNERKSSESMELLAYSIINLICKEA
                     ASGTYRGALETLQKMMSECIYHEGNAFVIMGSGEQLKRIKYDVDENNLKVFNVYFDNN
                     EELVTDGEPDVVCLSKQVWEDLLIKLKLENKENAVSETDLSSNKNNVDQFIECAKRNE
                     QTLFDNIRKSDFHVDSFKPGSTRSVILETQPNVCMEPHNLYDNQIDKVSPVTKNSQQV
                     KGHYGDKFKEMQMFLNQMSNALQQTPSLSESKEHTIDIQKITDAFVKEFRGVLIDKNG
                     NCSERLFNFYECCYKFLPRAQPQDKIESYNSALQAFSIFRSSTLNNNDVGFNFKLFPE
                     VKLSGENLETVFKYKKGSFVREIARINITLQKEEDGLYNLGGLDFKGCFFSGQNFSNY
                     DIQYVNWGTSLFDLDTPCIFNAPAYNKSNEKSLKPVSENGLSGVLTDRNNKIKLITGV
                     APFDDILFMDDDFDDSSSEDDPVENSPVVTSPVVSSSKSSFQ"
     gene            complement(4506..4581)
                     /locus_tag="OVW45_004248"
     tRNA            complement(4506..4581)
                     /locus_tag="OVW45_004248"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4546..4548),aa:Ile,seq:cat)
BASE COUNT         1312 a         1018 c          817 g         1474 t
ORIGIN      
        1 actaaagcac tgcccgtctc ctggttaacc ccatgtgcta taccgccggt atagacaatc
       61 agcgagcctc cggcgctaat attgctgcca gttgccgtac catctttctc aacaacctgc
      121 cggctcccgg tggaaatatt tgtcgtgtca gctttcccgc cgctcttgat attttgtgtt
      181 ccgctgttga tattggtacc cgtggcaata ccatgattat taatattctg tgtaccacca
      241 ttgattatgg tattggtcgc gtttctggta acatccatta ccccgccgtt atctattcgg
      301 gtcgcatcag ctttagcatt cgttaaaact gacattgttc ctttatcttt aataatcgtc
      361 ttatttgccg aaccatatgc gtttatgtct aaatgaccac cgttttccag caacacattg
      421 tctgccacgt gattgtggat ggagaatgca ccttcactat tcgtaccgct caccgtcgta
      481 ccgttagtgt ttgtttttaa aattgcacca tcgtgctggg taacatttgt tgccgtacca
      541 ccactaacat caagcacgcc accggaataa acttcaataa catccgagga gctggtgtaa
      601 tcaacaattt gcgtaccacc agaatagatc tgagtatttt ttgccgttga cttattattc
      661 agagactgag ttccaccttc aatcgtcgtg tccagcgcat ggctttcata tactctttgc
      721 tcaccgccat ttttaatggt ggttgttcct actgtgctct gttcaacata ctgtcgtcca
      781 ccatttatga ttgtgttcgt tgccatactt ccttgcgtga tgtcctgata accggactta
      841 tttatcgttg taccatcaac atgcccctga gttgttactg tctgccctcc accatcaatg
      901 atggttccat tcgcagtccc cccacttatg tgtgaattac cacccttaat tgtcgttcca
      961 ttgctgatac cacctgtata aacgtcctga ttgccactgt cgattatcgt accggtggaa
     1021 atgcccccgt catgaattgt ctgtcttcct ccgttaatgg ttgtattatt agactgcccc
     1081 atataattgt tattccttcc aacatcttga tatccaccag attcaataag acttccatta
     1141 gatactccgc cataaacact ctgctggcca tggttgataa tatgagtgtt atttgttgta
     1201 cctcgttcat ctactcgttg gttgccatct acagtctcat cgtttaccac accaataaca
     1261 ttagtagtaa aggccgccat cacgggcggg gcatatatca aggcagatat caataaggaa
     1321 agtactgagc ggcgacaata atggggactg gtcctgttca taaatttcat cctctgaaaa
     1381 gtgaatactg agtagcgttt aagcgacttt agctctgctg caacatcagc ccgcaggcac
     1441 cagaccgggg ggttcatcct gaagagacag cgcaagtgta ttgtgttcac cgctcatcaa
     1501 agacatcatg atgaaatgat gatgttccac ataagaaaga ggcatttttt aaacacagtg
     1561 cgctgaagtg tgattggata aaaaagtcaa tctattcagg aaatacgggc gtattctttt
     1621 ctttcgacaa tcaggccgtc ggcaaaataa aatgatttac ataatcgttt ctgatgaata
     1681 tcttctgctc acataaaaat cacacaataa ctttgagatc gcagattctt ttacttttac
     1741 agcattcgtc cccccattgt tgggcaaata tagattgggc cagagcacga aagttaatac
     1801 cacgtttgca cagctcctcc aacagcacga caagatgcca catactgcgc cccagtcggt
     1861 gcggtttaca gactacccgt gcgtccaccg ccgataatgt cctgagcagt tttttcagtc
     1921 cggacctcct gccacggttt tacgttgaag aggtaaccct gagcacgcag ttcttcagtc
     1981 aggcgtggtg caccgtaacg ctgttattgc tgggtaagat caaaaaactt tcaggcagct
     2041 aaggaaagtt gaaccagaca ttagatgaaa tatttcaacc aattacagca ccaatttaaa
     2101 ctcagtaaat cacagcacag atcaaggggt tacgtgaaag cgtagcccct ttttctttgg
     2161 tagtggcagc aaaatggttg tagtgtaaaa aataatcccg tttaatcaat caataataca
     2221 tattgtttca atctacgtta ttatctcttt gtaaaaatag ccatttatta atcattgaaa
     2281 actgctttta gaacttgata caacgggact agtcacaaca ggactattct caacgggatc
     2341 atcctcagag gaactatcat caaagtcatc atccataaat aaaatatcat cgaatggtgc
     2401 cacgcccgtg atgagtttta ttttattatt acgatcagtc aagactccac ttaaaccgtt
     2461 ttcgctcaca ggttttaatg atttttcatt actcttgttg taagcaggcg cattaaaaat
     2521 acacggagta tcaagatcaa acaatgacgt tccccagttc acatattgaa tatcatagtt
     2581 actgaagttc tgtccagaaa agaagcatcc cttaaaatcc aatccaccta aattatataa
     2641 accatcctct tctttttgga gagtaatgtt aattctggct atctcccgga caaaacttcc
     2701 ttttttgtat ttgaataccg tttcaagatt ttcaccagac agcttgactt ctgggaataa
     2761 tttaaaatta aaccctacat cattattatt caaagtagat gaacgaaaga tggaaaaagc
     2821 ttgtagtgct gaattatagc tttcgatttt atcttgaggc tgcgctcttg gtaaaaactt
     2881 atagcagcac tcataaaaat tgaataaacg ttctgaacaa ttcccatttt tatcaattaa
     2941 taccccccgg aattctttca caaaggcatc cgttattttt tgaatatcta tggtgtgttc
     3001 ctttgactct gacaaagatg gagtctgttg aagtgcattg ctcatctggt taaggaacat
     3061 ctgcatttct ttaaatttat ctccatagtg gcctttaact tgctgagagt ttttagttac
     3121 aggtgaaacc ttatctattt ggttgtcata taaattatgt ggttccatac agacatttgg
     3181 ttgtgtttct aaaataacac tacgtgtact acctggctta aatgaatcaa catgaaaatc
     3241 actttttctt atattatcga atagggtctg ttcatttctc ttagcgcatt caataaactg
     3301 atcaacatta tttttattcg atgacaggtc agtttcagaa accgcatttt ctttgttctc
     3361 cagctttagt ttaatgagaa gatcttccca gacctgcttg cttaaacata ctacgtcagg
     3421 ctcaccatca gtaactaact cttcattatt atcaaagtat acgttgaata cttttaagtt
     3481 attttcatca acatcatatt taatacgttt taattgctct ccagatccca taatgacaaa
     3541 ggcgttgcct tcatgatata tacattcaga catcattttt tgtaaagttt caagagcacc
     3601 gcgatacgtc cctgaggcag cttccttaca aattaaattt ataatactat aagctagtag
     3661 ctccatggat tcagaagatt ttctttcatt acaatttcca ctatctataa atgagctagt
     3721 gttaaattcg ttgcttttac taactaacat tgtctattcc tcagttaatg tctacatggc
     3781 tatttttaat tttattattg tttgtcacta taaaaaatcg ctcatttgag acaattgctg
     3841 gcattaacag cttcatatgc tatacatggt acttttaatt aaattagcac aagaatgtta
     3901 aatttaataa acaaaaagtt atttcgctgt atgataaaaa ccacccgtta taatttatta
     3961 gtgaaattcg tttttcgagt gttagaaatt tatatctaaa tagcgttgat taatgagcaa
     4021 agccagactc ctgtaacgct cacaagactc atctacctgc gacgggtatt ctggtcagta
     4081 gtagatgttt aaggcgtggc agagacattt catccttact ctacggcatt ggcccgcata
     4141 caaatcgtgg ttgtgacact cacttatcat cagtgagcga acagagaata gttcagtgat
     4201 ttgagtaatt aacctgatta aatgaagggg tataataaat gataatactc tggctttatc
     4261 gttaattact taattccaca tgtaagcaat ttgcccgctt ggcatagcag gcattttttc
     4321 caggtacttt tgaatgagta ctgatggata aatacattgc agtggcgtgc cacgtaccaa
     4381 aacaccagcc ctcattcgaa accacccacc gcacttcttc cttgaaatgg cgttagtcat
     4441 gaaatataga ccgccatcga gtaccccttg tacccttaac tcttcctgat acgtaaataa
     4501 tgatttggtg gcccttgctg gacttgaacc agcgaccaag cgattatgag tcgcctgctc
     4561 taaccactga gctaaagggc cttgagtgtg caataacaat acttataaac cacgcaataa
     4621 a
//
LOCUS       DAKISA010000199         4594 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.202, whole genome shotgun sequence.
ACCESSION   DAKISA010000199 DAKISA010000000
VERSION     DAKISA010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.202"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(240..3974)
                     /locus_tag="OVW45_004249"
                     /pseudo
     CDS             complement(240..3974)
                     /locus_tag="OVW45_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            complement(4050..>4594)
                     /locus_tag="OVW45_004250"
     CDS             complement(4050..>4594)
                     /locus_tag="OVW45_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528543.1"
                     /translation="IQNIGVNQIQTVGVNQVETVGSNQIIKVGSNQVEKVGIIRALTV
                     GVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFSVGKTMKENTGQ
                     TAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAPVINWNCGATQP
                     VPDAPVPKDLPPGMPDMRQF"
BASE COUNT          811 a         1557 c         1182 g         1044 t
ORIGIN      
        1 aatgcaggcg attgtgattt cgagtttaat tcagttgaac aaaataaaga tgctctacta
       61 ttccaatata tcgtcatatt attcgaatta aatctaacat tggttccttc aatatactct
      121 atatttatag tttcttcaga gcttgataaa aaatctatag tttctttcat ttgtgaatct
      181 tgttttaaat actcaacagc ctgattaaaa tcacttggat caccgttaac atggaatttt
      241 aaacccaaag aatctataaa ccgaacaggg tttaatggat atttatacag attccattca
      301 cctctcagcc ctatcggatc ctgggtgata taccttccct gcaacgggtc atagtaccgg
      361 ttgcggttgt agtacagccc cgactcctca tcatactgct gccccggcag acggagtgac
      421 tgctggaggt gctgcgcgct ggtttcgccc agcaggtttc cccattcgtc atactccccc
      481 tgccacgccg tttccccttc cgggctgatg agcgccagcg gcagtccccg gtggtcgcag
      541 tggtaaagat gaagtttcct ctccgggatg tattccgcct ccagctgcgc ggccatctgc
      601 tccgccgtca gcccgcactg cgcaagccat gcctcactct ccgcactcac cgctcccgcg
      661 cgcagttccc gctccagcct ccccagcatc accgacagct ccgccggtag cgtcacaccc
      721 gtgtcctcct gcaacacctc cgccaggctc cggtgccgcg ccttcgcctg ttcgccattc
      781 tctgtttcga ttctcaggag cggcgtgaag ctccccggct ggtataccgt ctggatacgc
      841 gtggtgccgg tctgtatcgt cgtcagcctg tcaccgtccc acccgtacca tgtcacctcc
      901 ggtttacgcg acagcgacat ccaccccgtc aggtcacgct cccgccgcca tacccgtttt
      961 cccgtccggc ggcccagcgg gtcatagagg taccggctct ccacctgtgg ttcgccgtgc
     1021 tgtatccgcg tgtagaacac caggcggtgc tggctgtcgt agtgatagtg gtgggtgcgc
     1081 tcgtcgtgca tccggataac cccttccggg atacggtccg tcttctccgc cagcctgccg
     1141 tattcatcgt agcgatagac atagtgcgca tcttccgcga tgcggttatc cggccacgcc
     1201 gtgagggtgc tgtccgggtg cagttccggg tccggcagcc gatttcctgc cgggtctgtt
     1261 gcatacggga tatcgatatc caggttcgct gccgtggtgt ggacgcccgt cagcctcccc
     1321 gtgtcgctgt aacggtactc ccggctctcc tgcgggccac tgatgcggat tagctgtccg
     1381 ttgtcgttcc agtcgtagtc acggtcaagc tgcggaaggt tcaggtgcct gctccggagc
     1441 tggccgctgg tattccaggc ggtggcaagt tcatatgcct ccccgccgaa gctgcgggcc
     1501 gtctcacggt gcagccggtc ccgcgtgtac tcgaccagcg gtgttccgcc cagcttcatc
     1561 cccgcaagat aaccgctgcc atacgtcagc cactccaccg gcggcaggcc gtccggctcc
     1621 tgacgggtcg ccagcccctg ttcgctgtac gcgtgccccg tctcatgctc ccacagcatc
     1681 tcccccgtct ccggggtctc caccgtctgc cgttcgcccg tcagacggcc tttatcgtca
     1741 tagccgtagt ggaccgccac acggtggcct tcactcaggt ggctgatttc tgtcagccag
     1801 ccgtggtcgt catactgcca ctgctccgcc ggttcaccgt tcaccgtgcg ccgggtgata
     1861 cggtccgacg catcgtaatg ccagagggtg ataagcccct catcttcact ctgcgtgagt
     1921 tttccggtca ggtcatactg gtaacgttgc gtccggccgt caaagccgcg ctgctccgtc
     1981 agcctgtcga ccgggtcata ccggaacgtg gactggctgc cgttctcgtt ggtcagcaca
     2041 gtgatgcgcc cggcggcgtc ataccccatg cttcgcgtca gaccgccctg cgtggtgctg
     2101 acggcctttc cccacgcatc atactgtatc tcactgcggc tgccgtccgg ggcaacgata
     2161 gcggtgaggt ctcctgcggc gctgtactca taacgggttt cacgcccctg cgcatccctc
     2221 tgactgacca gttgaccgcg cgggttataa ctgctgtaag tgctgatgcc ttcttcccgg
     2281 tgaacggcga tttgttgacc gtaccggtca tactcatacc gcgtcgtgta ccccgagcag
     2341 tccgtaaacg cctgaagctg accgtaccgg tttcgcgtta ttctccgcct caccccggtt
     2401 ccgtcttcca ccgcacaggg caggccactg cgggaagaat cataaaacca gcgcgtaatg
     2461 ttaccgttgc gcgaggtctc ttccgccagc ctgccctgac ggtcatattt ccggctgctg
     2521 cgtaaaccat ccggatatgt cactgacgtc agttgcaact ggttgttgta accataccgt
     2581 accgtcctgc cgtccggcag taccactgaa gtcagctttc cagacgccat atacagccgg
     2641 tactccgtcc gccgccccgc cgcatccgtc tgcgccttca gcctccccgc ctcatcatac
     2701 tcgctgcggg tgatgctccc gtcggcatgc tccttcttca ccacccgctt caggccaccc
     2761 tcgccttccg tgtacagcac ctcccgccgg ttcaggctgt ccgtgatgat tacccggctc
     2821 tctccgtact caaagcggta gtccagcccc tccgggttga cctgctccgt cacccgaccg
     2881 gtatcatcat accggtagcg gctctccggc ctccccgcat aatggtgcgc caccatccgc
     2941 ccggcgtgct ccgcatcata agtaaacccg cgaacctgcg tcccgctgcg gtcatacacc
     3001 gcccgcagtt caccaccggc cgtgtacgtg tagcgcgcca gcggcgcagt cggctgttca
     3061 tccgggtatg ccgggtcgtg tgtcagccat accgcctcca gccggatacc gttatcggca
     3121 ccgtactctg taccggcggg cagcgtgtcg gggaaaacct gagaagagga aacagaacgg
     3181 ggactggccg gggaagataa ggaagaggcg cgctgtttac gggacgcttc cgcccgctgc
     3241 gcctgcgtgc tcagcgccat gtgaaaacgg cggcccgcgc cgtccgtcac ccccgtcacc
     3301 gcgcccgcca cgtcaccctc cgccgcccgg tggaaagtca gtgagcgccc gaagccatcc
     3361 accacgaccg tcagtacccg gtatgcgggc ggctccggcc agctgagtat ccaccacggc
     3421 ccctgaaggc tgtttgtcgc caggtacaca tgcggactca ggcgaacatc ttccggcagc
     3481 acctgccaga gcgcgcttag cggctgcgaa ctgtgctgcg ccgccactcc gccccgcgcc
     3541 agccagagtg actcgctgcg gctgtagctt atctcgccgg gaaacagcgg ctcaaagtga
     3601 atgctcctgc cgccgctgtc gttgagtatc aggccttcat cgcgtatctg taagcggata
     3661 tcgaagggcg ctttccagcc gggaccaaac acccccaccg gcgcgggtgt tctggtccgg
     3721 tagctgctgt aggcacggaa aagaataaac ggcagcgggc agggcagcgc aaggtccgtc
     3781 tcgcccggaa gcaccttcgc acccagcacc gggttcaccg ggttcaccgg gttagcatag
     3841 gtaatccctc ccggacacac cgaacaggcc actcccgtcg gcgcacctat cagcacaccc
     3901 gctgaaccct gcacaatatc cagtcccttg cgggtcatgt cgccctgccg cgccgccggt
     3961 tttccgccgc ccatgaatca attccttctg cttttaatat ggttcatctg gtcactgcac
     4021 cagttccgtt ttaatttttt gcacctgact caaaattgcc gcatatccgg cattccgggg
     4081 ggtaaatctt tcggcacagg cgcatccggt acaggttgtg tggcaccaca gttccagtta
     4141 atcactggcg catcaccgtt cacatccgac tccccttcca gatgaatgtg cgtaccgttg
     4201 agaaatatgc ttccgtcctt cgtcagcacc agccttgcct taccacagca gagttcaaga
     4261 tgctcaccgg cagaataaac tgctgtttgt ccggtgtttt ccttcatcgt cttgcccacc
     4321 gagaaggtga cgttattccc tacattgacg ctgtagccca ttcccaccat cagtgattta
     4381 tgcagcccta cctgtgagga ctgcaacaac gccaccgagg tattcataat gccgcctacc
     4441 gtcgtctggt aagccacacc caccgtcagc gcacgaatga tccccacctt ttcaacctgg
     4501 tttgatccca ctttgataat ctggttactg cccaccgttt ccacctggtt gacaccaacc
     4561 gtctgtatct gattaacgcc aatgttctgg atct
//
LOCUS       DAKISA010000200         4566 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.203, whole genome shotgun sequence.
ACCESSION   DAKISA010000200 DAKISA010000000
VERSION     DAKISA010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.203"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..180
                     /locus_tag="OVW45_004251"
     CDS             <1..180
                     /locus_tag="OVW45_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCV5528544.1"
                     /translation="RLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVEL
                     HDKVIGHYLNIKHYQ"
     gene            214..908
                     /locus_tag="OVW45_004252"
     CDS             join(214..460,460..908)
                     /locus_tag="OVW45_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element ISEc30 family transposase"
                     /protein_id="HCV5528545.1"
                     /translation="MARVNIHCPRCQSAQVYRHGQNPKGRDRLRCCDCHRVFQLTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVTHADVA
                     FICELDEQWSYVGSKARQHWIWYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQCIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(1027..1836)
                     /gene="murI"
                     /locus_tag="OVW45_004253"
     CDS             complement(1027..1836)
                     /gene="murI"
                     /locus_tag="OVW45_004253"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCV5528546.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNTNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNPTINIFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNLFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(1870..3330)
                     /locus_tag="OVW45_004254"
     CDS             complement(1870..3330)
                     /locus_tag="OVW45_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCV5528547.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFAGAIY
                     LRHSQPDKKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            complement(3370..3606)
                     /locus_tag="OVW45_004255"
     CDS             complement(3370..3606)
                     /locus_tag="OVW45_004255"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528548.1"
                     /translation="MQICWHKFCRYCDVKLRKISLYDHRLIMNAEEVLKHVDENTIGV
                     VTTLGITCFYFCKADCVSVFKISVKLILVFKFIS"
     gene            complement(3587..4072)
                     /locus_tag="OVW45_004256"
     CDS             complement(3587..4072)
                     /locus_tag="OVW45_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCV5528549.1"
                     /translation="MKNKSGNSYYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWIDDEIRELLNLSIDRNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            complement(4310..4486)
                     /locus_tag="OVW45_004257"
                     /pseudo
     CDS             complement(4310..4486)
                     /locus_tag="OVW45_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         1442 a          952 c          883 g         1289 t
ORIGIN      
        1 cgcctgaagg gaaagctgca cgttatcagc aagcgttaca ctcagcgcat tgagcgacat
       61 aatctgaatc tgagacaaca tctggcaagg ctgggacgga agtcactgtc gttctcaaaa
      121 tcggtggagc tgcatgacaa ggtcatcggg cattatctga acataaaaca ctatcagtaa
      181 gttggagtca ttaccggttt ttgaggttat ctcatggcca gagttaatat tcattgtccc
      241 cgttgtcagt cagctcaggt ttaccgccat ggtcagaacc ctaaaggccg tgacagactt
      301 cgctgctgtg actgccaccg tgtgtttcag ctcacttata cttatcaagc acgtaagccg
      361 ggtatgaaag agctgatcac tgaaatggcc tttaatggtg ccggggttcg cgataccgcc
      421 aggacactga aaattggtag taacaccgtc atccggactt taaaaaactc gcgccaaagc
      481 gaataacttc ttcgcctgtt acccatgctg atgtggcgtt tatctgcgag cttgatgagc
      541 aatggagcta cgttggcagt aaagcccggc aacactggat ctggtacgcg tacaacacca
      601 aaacaggcgg tgtactggcc tacacttttg gtccccgaac cgatcaaacg tgccgggagc
      661 tactggcact gcttacaccc ttcaacatcg gcatgctgac cagcgatgac tggggcagct
      721 atggccggga ggtgccgaag aataagcatc tgaccggcaa aatattcacc caatgcattg
      781 agcgtaataa tctgacgcta cgcacccgca ttaagcggtt gggtcgtaaa acaatctgct
      841 tctcacgctc agttgagatc cacgaaaaag tgatcggagc gtttatcgaa aaacacatgt
      901 tctactaatt ggatgcacta cccagtgtgt aactgggttt gcccataaaa tgcaccttac
      961 tcagttggtg tccaactttt ggggtgcagt tcagaaggac ggggttttac ggtgcaccgg
     1021 ataaatttaa aactgcctga tactgaatgt atgaggtttc gtaatgctca attcccctag
     1081 taccgttttg tagatctcca tacttccact agtatttatt tgcaaagttc ctatatttga
     1141 ggagtttaat aaatttagct gtcccaagtg atttttaata gagatagcct gatcatgtgc
     1201 aggattaata aattttatat ccggtgcaac tgcttcaaat aaattctgaa caattggata
     1261 gtgagtacag ccgagaataa tttcatttac tggatggagg gaaattaaat ggttaacatg
     1321 ttttttgata tcattcaata ttgcaggagc attaagatta ccttcctcaa ttaacattgc
     1381 aagatttttg cttggttctc caaatatatt tattgttggg ttcaattttt ttattagctc
     1441 tttgtgacac cccgttttaa tagtaaactc ggtagcgata atacccacat ttttatactg
     1501 ctggcgaatc acatcttcta ctgcaggttc aatgatggat aagataggaa acggaaagct
     1561 cttagcatat ccgctgtatt cttcggattc caaagttgag gatatagtat tacatgcaac
     1621 agcaactagt ttgacattcc ttgactgtaa aaaagtcagc atttttttag tcaaactgat
     1681 gatatcatcg gcagaacggt tgccataagg tgcattcgta ttatctccac aatagataat
     1741 atcttcgcca ggaagcaaat taattatttc ctttaccgat gtcaatccac ctacacctga
     1801 gtctattaca ccaattggag attcagcact aaacatattt aacctcatct tttgtagctt
     1861 attgaaaatt taattactag ttcttttttc ccaagaaaat ggttcgaaat cagagtttgg
     1921 atctttccag tcagattttc ggaaggcgta aattaatatt ggaattaata aaaatagggc
     1981 aactaatact aacaaataaa gaacatagct ggttggacta ccagttggga tttgctcggg
     2041 cggaataaaa cttaatacaa aagccagaaa agaccctaag aatccaagtg ttgcccagaa
     2101 gaataaagca ggggctcgat atgggcgttt tttatcaggt tgagaatgtc gtagatagat
     2161 agcaccagcg aacatgagca tatacataac taaatagagc ataactgtta actgtgataa
     2221 aatctgaaaa gctgcttgaa ctgaaggcat tatgacaaaa gtaacagaaa gaactgagac
     2281 aattatacct tgtacaaaca tcagatggtg ccccattcca tgacgattgg ttttttggaa
     2341 aaacttaggt aaataaccag ctttcgcgat cgctaataca cctgtattag gccctgcaat
     2401 ccaagtaacc accccaccca gtacgccaat tgctagcata aatgccatca cagagccaag
     2461 ccatggcact ccagcccatt cgaacagcat atcgtaggca accaacaaac tttgagttag
     2521 actaatttta tctgtaggaa taataaaggc gacccctaaa gtactgagca ggaatattgc
     2581 aatagtacct atagaagcga tggtaattgc aattgggtat tttttagttg gattatctaa
     2641 atcgttaaca tggaccgcat tcatttccat ccctgcataa gcaagaaata tgctggcggc
     2701 aagaacaata ttctggaagt tagctatatt agggaaaaga tcaccccaac ctaatgggat
     2761 caccgctggt ttaccgctga accacagata ggagaaacca agaataacaa ggataatagc
     2821 tggaataatt gtccctataa taccgcacca cttagatacc tgcgagaaaa taccaacgcc
     2881 acgtaatgca acacaaatag ctccccagta aacaaatagt acgaaaaata aaatatacca
     2941 tttattactg gcaattgatt ctgccacccc catatcaaca tcaataaatg caaccgagac
     3001 tgcaccaaat gtcagtactg ttggaaagta ggggatgact tgtagccagg tcataagcat
     3061 tcctacaaaa ccccagcgag ccccaaatgc ttctcctatc catcgaaatg cccccccttt
     3121 ttgtggatat gttgtagcta gctcagccgc aacaaaggca acaggtacta aaaacataat
     3181 tgcagcaaag gtatagtaaa aaatggcacc taagccgtat tctgcttcag aaggtataaa
     3241 tcgtagactt actacagcag ttatattcat gatagcgagt gtccccactg tcattttctt
     3301 acttttgggt gcgatattag cattcgacat ttttatactc tcgaatattt ttaaaataga
     3361 aacatataat tacgatataa atttaaaaac taatattaac ttgacactta tcttaaaaac
     3421 actaacgcaa tctgctttac aaaaataaaa acaagtaata cctaaagtcg taacgacacc
     3481 aatagtgttt tcatcgacat gcttcaaaac ctcttcagca ttcattatca ggcgatgatc
     3541 ataaagtgaa attttacgca atttcacatc acagtaacgg cagaatttat gccagcatat
     3601 ttgcacagga tcgcaaatca ggttcggttg atttaccttg tacttcgcgt tttttacacc
     3661 actgccattt taacgccaag cggccgagca tagcagcttc agatgaacca atggttgagc
     3721 aacctaaggt gtgtcgcgaa tcggggaaac tccacgaatc ggaaagcata cgcacacagc
     3781 gaccttcagg ttccgccatt tgtggatatt catctttata aatcatgttt ctatcaatag
     3841 ataaattaag caactcacgg atttcatcat caatccatgt ttgacagaaa gtagctaagt
     3901 tttgtcgtga atccccatca aggatgagct catcacgtat ggcgctgaat acatttcgag
     3961 atttcttctc atctacacgc aaatgatatt tatgcaatat aaatgaaagc tcaagtgagc
     4021 tatgaatatc gaccgcttct ctataataag aattgcctga tttatttttc ataataaacg
     4081 ccactcaata taatcagatt aaacattaca aacaaaatat ggattttgca attttaaaac
     4141 tatcaatttt agatatgttt taattaatat aaaactataa acatatcaaa aaattacttt
     4201 attatggtca tgatagatgt gtttcaatgc caacctggtc tattcctaca tatgtgagag
     4261 catatttaca aaaaaatcgt tattgtgatt tcaacggtga tgcatccaat tagtagaaca
     4321 tgcatttttc catgaatgcc ccgatgactt tttcatgaag ctcaatagaa cgtgaaaaac
     4381 agagcgtttt gcgggccagc cgtttgattc tggttctcag cgtcaggtta ttgcgctcaa
     4441 tgcgctgagt aaaaatcttg ccggtcaggt gcttttcttt cggaagtgaa gtggtcaaca
     4501 aaaactggcc accgcgttag agtttttcca gtatcgattt tccgattcgt ttgggggtaa
     4561 cccacc
//
LOCUS       DAKISA010000201         4479 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.204, whole genome shotgun sequence.
ACCESSION   DAKISA010000201 DAKISA010000000
VERSION     DAKISA010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4479
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.204"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..1134)
                     /locus_tag="OVW45_004258"
     CDS             complement(<1..1134)
                     /locus_tag="OVW45_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCV5528550.1"
                     /translation="MFFSGLFQRKSDVPVTTPAELADAIGLSYDTYTGKRISSQRAMR
                     LTAVYSCVRVLAESVGMLPCSLYKISGTLKTRAVDERLHKLVSAKPNGYMTPQEFWEL
                     VIVCLCLRGNFYAYKVKALGEVVELLPIDPGCVEPKLNSQWQPVYQVTFPDGSVDVLT
                     QNEIWHVRTLTLDGLVGLNPIAYAREAISLAAATEEHGARLFSNGAVTSGVLRTEQKL
                     TPDAYERLKKDFEECHTGLGNAHRPMILEMGLDWKSMALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRKSKQGVYY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSSNDCRDLEDMNPRP"
     gene            complement(1134..1316)
                     /locus_tag="OVW45_004259"
     CDS             complement(1134..1316)
                     /locus_tag="OVW45_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528551.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTRQSVGGGK"
     gene            complement(1328..3061)
                     /locus_tag="OVW45_004260"
     CDS             complement(1328..3061)
                     /locus_tag="OVW45_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCV5528552.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVICCAFGWVNKG
                     TRLRRFREVYTEIPRKNGKSAISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPA
                     RLMCKRTPMLTEAFGIEVNASNMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHAT
                     DALYTTMLTGMGARRQPLMWAITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIY
                     TVDEGDDWTGPQVLEKANPNIGVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSA
                     RSAYFNLVSWQSCEDKSLTLEQFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYY
                     SVAPRFWVPYDTVYSVEKNEDRRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAAN
                     KISPVSESPIDPFGATGLSHDLADEDLNPVTIVQNFTNMSDPMKELEAAIESGRFHHD
                     GNPIMTWCIGNVVGKNMPGNDDVVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLS
                     DHIESYGIRSL"
     gene            complement(3058..3552)
                     /locus_tag="OVW45_004261"
     CDS             complement(3058..3552)
                     /locus_tag="OVW45_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCV5528553.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEDLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIAIQG
                     NTITGAMGGRVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            complement(3674..4024)
                     /locus_tag="OVW45_004262"
     CDS             complement(3674..4024)
                     /locus_tag="OVW45_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCV5528554.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(4067..>4479)
                     /locus_tag="OVW45_004263"
     CDS             complement(4067..>4479)
                     /locus_tag="OVW45_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCV5528555.1"
                     /translation="LSWAVNHYRDNAITYKEQRDKNARELKLANATITDMQQRQRDVN
                     ALDAKYTKELADAKAENETLRADVAAGRRRLHIKAVCQSVREATTASGVDNATSPRLA
                     DTAERDYFTLRERLMTMQMQLEGAQEYIRTQCIK"
BASE COUNT          961 a         1327 c         1128 g         1063 t
ORIGIN      
        1 gggacgcgga ttcatatctt ccaggtcgcg gcagtcattg gaagagtaaa ttccccagtt
       61 gatcccggtg gcgtaggctt caaaacggga cttcatatcc ccgcgcagta acgccccggc
      121 gttaaatttg gcgtaataaa cgccctgctt actttttcgt accagtccgg tgttgatccg
      181 ctgttcgatg cgggtcagat acggcaccag tgaatagttg ataaatccca gccccagctc
      241 ttcgatattg ttgaaggtgg cgcgatcggt gttctgcacc atgtgcaacg gcacccggaa
      301 cagacgacag atttcttcca gctgaaactt gcgggtttcc aggaactgac tgtcctcggc
      361 gttcagcgcc atcgacttcc agtccagccc catttccaga atcatcgggc ggtgagcatt
      421 gccaagcccc gtgtgacact cctcaaaatc tttcttcaga cgctcgtaag catccggcgt
      481 gagcttttgt tccgtacgca acacaccgga tgtcaccgca ccattactaa acaacctggc
      541 accgtgctcc tcggttgccg ctgccagtga aatggcctca cgcgcatacg caatgggatt
      601 cagcccgaca agtccatcca gcgtcagggt gcgcacatgc cagatttcat tctgggtcaa
      661 cacatccacg gaaccatccg gaaacgtcac ctgataaacc ggctgccact ggctgttcag
      721 cttcggttcc acacagcccg gatctatcgg aagaagctcc accacttctc ccagtgcctt
      781 taccttgtag gcgtaaaaat tcccccgtag acacaggcag acaataacca gctcccagaa
      841 ttcctgcggt gtcatgtagc cattaggttt tgccgaaacc aacttatgca gtcgttcatc
      901 caccgcccgt gttttcaggg taccgctgat tttgtagaga ctgcagggca gcataccaac
      961 agactctgcc agcaccctga cgcaagaata gaccgccgtc aggcgcatgg cccgctggct
     1021 actgatccgc tttccggtat aggtgtcgta tgacagcccg atagcatcag ccagttctgc
     1081 tggcgtggtc accggcacat cgctttttcg ttgaaataat cccgaaaaga acactattta
     1141 cctccgccga cagactgccg tgtacggtcg agatatcgcg ccaccagcca cgaccagaac
     1201 aggcacagcg ccccggcaac aacaaaaccc gccgggggat aaatcagcca ggcaccatac
     1261 gccagcaaaa gcgcacccag cacgcccacc agaggcgcga gaatcagcat gatcataatt
     1321 acctcagtta aagcgagcgg atcccgtagg actcaatgtg gtcagacagc gtgtcttctt
     1381 tctcgtacag catggctctg ccaaccgcca taatcagcgc aactgcacca tcgattttgt
     1441 tttccgcctg ctctttgacg ggtttcacca catcatcgtt acccggcatg tttttgccga
     1501 ccacgttgcc gatacaccag gtcatgatgg gattgccgtc atgatgaaaa cgtcccgatt
     1561 caatcgctgc ttccagctct ttcatcggat cggacatatt ggtgaagttc tggacgatag
     1621 tgacgggatt caggtcttca tcagcaaggt catgtgacaa cccggtcgcc ccgaaggggt
     1681 cgatgggtga ctcactgacc gggctgattt tgttcgccgc tttggcctcc tcgaggatgt
     1741 agcgataatc cacctccgca ccatcggtaa cggtcagaac gcccatttcc acccatttct
     1801 gaaagcgttc ggctgtccgt cgatcttcat ttttctcgac gctgtacacc gtgtcatacg
     1861 gtacccagaa acgcggggcc acactgtagt aatgcgtttt accgtcaatc tcgcgggtat
     1921 aaagtcgcgc catgctgttc atatccagct tacgcgccag gtcaaaggcc agaatgcacg
     1981 gctgcccctc gaactgctca agggtcagtg atttatcctc gcagctctgc cagctcacca
     2041 ggttgaaata cgccgaacgc gccgacaccc agatattgag gtgttttgtt ttaaagacgt
     2101 ttgccagacg ggcgttattt ttcgcacgct gctgctgact taacaaaaat tcgcgataaa
     2161 ccgacacgcc aatatttgga ttggcttttt ccagcacctg cgggccggtc cagtcgtcac
     2221 cttcatcaac ggtatagatg atcccgaaca gttcatcgtt aggcaccgag ccgttgagca
     2281 tctcgatgac ttcccgccgc ttgtcgtagc acggcccctc aatgttgtac ccggcggtgg
     2341 tgatggccca catcagtggc tgacgtcgcg cccccatccc ggtaagcatt gtggtataaa
     2401 gcgcatcggt ggcatgctcg tgatattcat caaccacggc acagtggggt gatgaaccat
     2461 cacctgggtt gccgatcagc ggttcaaacc gcgcgccatc ctccggacgg ttcatgtttg
     2521 aggcgttaac ctcaatcccg aacgcttccg tcagcatggg tgtgcgttta cacatcagtc
     2581 gcgccgggcg aaagacttcc cacgcctgtt tctctgtcgt ggcaccggaa tacacttccg
     2641 cgccaaactc gttatcacag gcaaaacaat acagggcaac accggcagag attgctgatt
     2701 tgccgttctt acgggggatt tcggtgtaca cctcccggaa gcggcgcaac cgggtgcctt
     2761 tattgaccca gccaaacgca cagcagatca caaatagctg ccacggctcc agcgtgatgg
     2821 gcatccgttt gaatgcccac tcccccttgg tgtgcggcaa cagctgaata aatttcgcgg
     2881 cccgttcagc caggtccttg tcgaagcggt aacgaaacga cttacttttt tccgccatca
     2941 ggtcatcaag atggcgctgg caggcctgaa tcacaaactg gcaggcaaca atctttccgc
     3001 gcacgacatc ccgggcatac tgatttgcag catttacgtt ggggtaagat ttccggctca
     3061 tgattcgata attttcagaa acgggttagt ggctttcttc tgccccgcca ggccaatcag
     3121 acgctggcgg ctgctggggt cgagtccgag cattgccccc gtgctgctca tctcggactc
     3181 ctgttctttt ttggcggtca gctccggatt tttgaccctg ccgcccattg caccggtgat
     3241 ggtgttgccc tgtatggcaa tatttttcac ggcacgtcgc cagaactcat aggccacgca
     3301 ccaccgctca agtaccgcca ggtcagtcac gcacagcagg ccctgaccgc agagttcttt
     3361 ggttgtcagt tgccacatga tcgtggcgag agggagatct tcttcagcga accactctgg
     3421 tggctcaaca cctttgatgg gcgtaaaaac aggttcatct ttgttcaggg ctcgcttgcc
     3481 ggggtttccg gccagcgcct tgcgcgccgt tggcttgggg cgacgcccgg aacgccccgc
     3541 cgttccagcc atatgcggca ctcctggtta aatttcattt ttcgcgggta taaaaaaacg
     3601 atggggcggg cagtccggaa gacgtcaggt cacagggatt tgccccgccc ctccctacaa
     3661 gtgagaataa ttatcaccgg attcgttcgc gcgctgtttt cgctttatgg cagggccagc
     3721 acagactctg caggttgctg tctgcgtctg ttccgccatg cgctttcggg atgatgtggt
     3781 cgacggtttt cgcctcgctc accacaccga cacgcagaca caactgacac agacctttat
     3841 cgcgcttcag aatacgggca cgaatcaccg tccattttga gccatagcca cgctggtggc
     3901 ggctcagtcc gcgctggtgc tgcacccagc cttcaccgcg atgtttatcg cagtaacccg
     3961 aactgtctgt ggttgtacct gcacatccac gcttacggca ggcacgtggg attagtgatg
     4021 gcataaatac ctcataccct gcgaaatgtt taccacgata aaaaggctac ttaatgcact
     4081 gagtgcggat atactcctgt gccccttcca gttgcatctg catcgtcatc agccgctctc
     4141 tgagggtgaa ataatcccgt tcagcggtgt ctgccagtcg ggggctggtt gcattatcca
     4201 cgccggaggc ggtggtggct tcacgcactg actgacagac tgctttgatg tgcaaccgac
     4261 gacgaccagc ggcaacatca gcgcgcagag tttcattttc agctttcgca tcagctaact
     4321 ccttcgtgta tttagcatcg agtgcattaa catcacgctg tctctgctgc atgtcagtaa
     4381 tggttgcgtt cgccagcttc agttctcttg cgtttttgtc gcgctgctct ttgtaggtaa
     4441 tggcgttatc acggtaatga ttaacagccc atgacaggc
//
LOCUS       DAKISA010000202         4464 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.205, whole genome shotgun sequence.
ACCESSION   DAKISA010000202 DAKISA010000000
VERSION     DAKISA010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.205"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..296
                     /locus_tag="OVW45_004264"
     CDS             <1..296
                     /locus_tag="OVW45_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCV5528556.1"
                     /translation="GNWMFNLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARV
                     VYDFGPQMADLNLAFGVKNIFDQDYFIRSYDDNNKGIYAGQPRTLYMQGALKF"
     gene            341..1243
                     /gene="fecB"
                     /locus_tag="OVW45_004265"
     CDS             341..1243
                     /gene="fecB"
                     /locus_tag="OVW45_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HCV5528557.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIASVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            1240..2238
                     /gene="fecC"
                     /locus_tag="OVW45_004266"
     CDS             1240..2238
                     /gene="fecC"
                     /locus_tag="OVW45_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HCV5528558.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            2235..3191
                     /gene="fecD"
                     /locus_tag="OVW45_004267"
     CDS             2235..3191
                     /gene="fecD"
                     /locus_tag="OVW45_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HCV5528559.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPYTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            3192..3959
                     /gene="fecE"
                     /locus_tag="OVW45_004268"
     CDS             3192..3959
                     /gene="fecE"
                     /locus_tag="OVW45_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HCV5528560.1"
                     /translation="MTLRTENLTVNYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
BASE COUNT          904 a         1247 c         1276 g         1037 t
ORIGIN      
        1 caggaaactg gatgttcaat ctgaacagcg atttccagtc cagccagttt gcggataacg
       61 ccaatacggt gaaagagagc gccgacggca gtaccggccg cattcccggc ttcatgctct
      121 ggggcgcacg cgtggtgtat gactttggcc cgcagatggc agatctgaac ctggcattcg
      181 gtgtgaaaaa catcttcgac caggactact tcatccgctc ttatgacgac aacaacaaag
      241 gcatctatgc aggccagccg cgcacgctgt atatgcaggg ggcgttgaag ttctgatgtg
      301 ttaacgcccg gcttgccggg cttttagctg gaatgtgatt atgttagcat ttatccgttt
      361 tctttttgca ggcctgctgc tggtgatcag ccacgccttt gccgccacgg ttcaggacga
      421 acacggcacg tttacactcg aaaaaacgcc acaacggatt gtggtgctgg aactctcgtt
      481 cgccgatgcg ctggccgccg tggacgtcag cccgatcggt attgccgacg ataacgatgc
      541 aaaacgcatc ctgcccgaag tgcgtgcgca cctgaaaccg tggcagtccg tcggaacgcg
      601 cgcgcagccg agcctggaag ccattgccgc tctgaaacca gacctgatca ttgccgacag
      661 cagtcgccat gcgggggttt acatcgcctt gcagcaaatc gcgtcggtac tgctgcttaa
      721 gtcccgcaac gaaacctacg ctgaaaattt gcaatctgcg gctatcatcg gcgaaatggt
      781 gggtaaaaag cgagagatgc aggcacgtct ggaacaacat aaagagagga tggcgcagtg
      841 ggccagccag cttcccaaag ggacacgcgt ggcctttggc acatcacgcg aacagcaatt
      901 caacctgcat actcaggaga cctggaccgg cagcgtgctg gcctctctgg ggctgaacgt
      961 tcccgctgcg atggcgggtg cgtccatgcc gtccatcggc ctggagcaac tgctggcggt
     1021 caatcctgcc tggctgctgg ttgcccacta tcgcgaagag agcattgtta aacgctggca
     1081 acaagatccg ctctggcaga tgttaaccgc cgcgcagaag cagcaggttg cttcggtcga
     1141 cagtaacacc tgggcgcgga tgcgcggtat ttttgctgca gagcgtattg ccgctgacac
     1201 ggtaaaaatc ttccaccatc agccgcttac cgttgtaaaa tgaccgcgat aaaacacccg
     1261 gtgctgctgt gggggcttcc cgttgcagca cttattatta ttttctggct gagtctgttt
     1321 tgctactcgg ccattcctgt ttccggagca gatgcaaccc gcgccctgct gcctggacac
     1381 acgccaacgc taccagaagc gctggtgcaa aaccttcgtt tgccacgaag cctggtcgcc
     1441 gttctgatcg gcgcaagcct ggcgctcgcg ggcacgctgc tgcaaaccct gacccacaac
     1501 ccaatggcct ctccttcact gctcggcatt aacagcggcg cggcgctggc tatggcgctt
     1561 accagcgcgc tgagtccgac gccgattgca ggctattctc tgtcgttcat cgcggcatgc
     1621 gggggcggcg tgagctggct gctggtcatg accgcaggag gcggatttcg tcatacccat
     1681 gacagaaaca aactgatcct cgcgggtatc gcgctgtcgg ccttttgtat gggccttacc
     1741 cgcatcaccc tgctgctggc cgaagatcat gcttacggca tcttttactg gctggctggc
     1801 ggagtgtccc acgcccgctg gcaggatgtc tggcagctct tgccggtggt ggtcactgca
     1861 gtccctgtcg tgttgctgct ggcgaatcaa ctgaacctgc tcaacctcag cgacagcacc
     1921 gcccatacgc tgggagtgaa cctgacgagg ctacgtttgg tcatcaatat gttagtgctg
     1981 cttctggttg gtgcgtgcgt cagcgtggca ggtccggtgg cgtttatcgg tctgctggtg
     2041 ccacatctgg cgcgcttctg ggcaggcttc gatcagcgca acgtactgcc agtgagcatg
     2101 ctgctggggg ccacgctgat gctgctggca gatgtactcg cacgcgcgct ggccttcccc
     2161 ggagatctgc ccgcaggcgc agtgctggcg ctgattggca gcccttgctt tgtctggctt
     2221 gtgaggaggc gaggatgaaa attgcgctgg ttattttcat cacccttgcc ctggcgggct
     2281 gtgcgctgtt atcactccat atgggagtga tccccgtgcc gtggcgcgcg ctgctgaccg
     2341 actggcaggc cggacgcgag cattattatg tattgatgga gtaccgactg ccgcgcttgc
     2401 tgctggcact gtttgtcggt gcagccctcg ccgtggcggg cgtgctgata caggggattg
     2461 tgcgcaaccc tctggcatca ccggatattc tcggcgttaa ccatgccgcc agcctggcct
     2521 ctgtgggggc tctacttctt atgccgtcac tgcccgtgat ggtgctgccg ctgctggcct
     2581 ttgcgggcgg catggcgggg ttgatattgc tgaagatgct ggcaaagacc caccagccga
     2641 tgaagctggc actcaccggc gtggcgcttt ctgcatgctg ggccagcctg acggattatc
     2701 tgatgctctc gcgcccgcag gatgtgaaca acgccctgct gtggctgacc ggcagcttat
     2761 ggggccgtga ctggagcttt gtgaagattg ccatcccgct gatgatttta tttctgccgc
     2821 tgagcctgag tttttgccgc gatctcgacc tccttgcact cggcgatgcg cgcgccacca
     2881 cgctcggtgt gtcggtgccc tatacccgat tctgggcttt gttactagct gtcgccatga
     2941 catctaccgg cgtggccgcc tgcggcccga ttagctttat tggtctcgtg gtgccgcata
     3001 tgatgcgtag catcaccggt ggacgtcacc gcagactgct gcctgtttcg gccctgacag
     3061 gtgcgttgct gttggtggtt gccgatctgc tggcgagaat tattcatccc ccactggagc
     3121 tcccggttgg cgtgctgacc gccattatcg gtgcgccgtg gtttgtctgg ttgcttgtga
     3181 gaatgcgata aatgacttta cgaactgaaa atctgacggt caattacggg acagacaagg
     3241 tacttaacga cgtttcactc tcactgccaa cggggaagat caccgccctg atcggtccta
     3301 acggttgcgg gaaatcgacg ctgttaaact gtttttcgcg gcttttaatg ccgcagtctg
     3361 gcaccgtatt tctcggcgat aatcccataa atatgctctc atcgcgccag ttggcccgca
     3421 ggctttcgct gctgcctcag caccatttaa cgccagaggg gatcacagtc caggagctgg
     3481 tttcgtatgg tcgtaatccc tggctgtcac tctgggggcg tctctccgct gaagacaatg
     3541 cacgagttaa tgtcgccatg aaccagaccc ggatcaatca tcttgccgtt cgtcggttaa
     3601 ccgagctttc cggcggtcag cgccagcgcg catttctggc aatggtcctg gcccagaata
     3661 cgcccgttgt attacttgat gagccaacca cctatcttga tatcaatcac caggtggacc
     3721 tgatgcggtt gatgggcgaa ctccggactc aggggaaaac ggtggtcgct gtgctgcacg
     3781 accttaatca ggctagccgg tactgcgatc aactggtggt aatggcaaac ggacatgtta
     3841 tggcgcaagg cacaccagaa gaggtgatga ccccaggatt gctgagaaca gtattcagcg
     3901 tggaagcgga aatacacccc gagccggtat ctggcaggcc gatgtgccta atgaggtaga
     3961 ttgcacaggc cgtaagaacc aaaccacgac tgaatgaaac tggactggcg ccagcaagcc
     4021 tgttcagact ggggctgaac ttttccggac tctgaaagat taccaatact catcgtccat
     4081 ccgcttgctt taggctgaca ggttcataat caacgcaaac cagagctgta caggcttggg
     4141 cgcggctttc aaaccagtcg tgatcacggc aatcaatttt gaactctgct taacggacat
     4201 ttctgtataa cccttacggc aacgaaaaac gcgaagttaa aattttagaa acccaaaaac
     4261 gtgacatgac taagtttaga tttcaggggg ggagatcaaa aaatttcgct ctgtgccaga
     4321 gcggacattc acggagctgg ttcattacca atgaggttgg gcttttgagg ataaatcaat
     4381 gatcagacgc caacgtaaat caaaagcaac cctggaacgg aattgctaat ccagtttctg
     4441 accatcgatt tttctaaaaa gtgt
//
LOCUS       DAKISA010000203         4406 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.206, whole genome shotgun sequence.
ACCESSION   DAKISA010000203 DAKISA010000000
VERSION     DAKISA010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.206"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(139..3654)
                     /locus_tag="OVW45_004269"
     CDS             complement(139..3654)
                     /locus_tag="OVW45_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCV5528561.1"
                     /translation="MDENALGFTSYWRNSLADAESGKGSFERKDAKNFTHWHGIAAGR
                     LDEAIVSKFFKGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLIVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQGISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4106..>4406)
                     /locus_tag="OVW45_004270"
     CDS             complement(4106..>4406)
                     /locus_tag="OVW45_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCV5528562.1"
                     /translation="TVNKALRVMGYDTTQDVCGHGFRAMACSALIESGLWSRDAVERQ
                     MSHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRDKGISPFEYAKINNPLK"
BASE COUNT         1142 a         1128 c          905 g         1231 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagatgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg ataccttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca ttaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcgtcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaggagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aatcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcacct gcagaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc ttaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt tggcgtcttt ccgttcaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggt aaaccctaaa gcattttcat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aaccaccata tggacaacta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcggtat caaatgtact
     3781 ggttgtacaa ggttcagaac acaagcacat tcaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa tgactgcctg tgttggacaa ggataccaga
     3901 acagccgaaa acaccggggg ttattacctg ctctcatgga aaccagatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacat gacgaaggga ctttaatgga agccggagta
     4021 acttgccaga acatcaatct tcgtcatcat ttacaaagaa aaataatcag ttaaagcagt
     4081 ccgggcaatc agcccggact gcttactatt ttaatggatt gttaatcttt gcatattcaa
     4141 acgggctgat acccttatct ctgttcgcat ccagaaaatc tgcccaccac tgtagcatca
     4201 gtcgacgttc ttccagatgt tctgctttat ggatatacgc agcacgtaca ccattacgct
     4261 cctgatggct catctgacgt tccacagcat cgcgggacca caaacctgat tcaatcaatg
     4321 cactgcacgc catcgcccgg aatccatggc cacagacatc ctgggttgtg tcatacccca
     4381 tcaccctcag ggctttattt acagta
//
LOCUS       DAKISA010000204         4405 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.207, whole genome shotgun sequence.
ACCESSION   DAKISA010000204 DAKISA010000000
VERSION     DAKISA010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.207"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..585)
                     /locus_tag="OVW45_004271"
     CDS             complement(<1..585)
                     /locus_tag="OVW45_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase N-terminal
                     domain-containing protein"
                     /protein_id="HCV5528563.1"
                     /translation="MNTHISVSTIPHLTGWHAINWKACHARVRKLQLRIAKATRQQQW
                     RQVRELQRILTRSFSGKAVAVRRVTENTGKRTPGIDGKIWHTPKEKWGGVCSLNLRGY
                     RPQPLRRIHIPKSNGKTRPLGIPTMRDRAMQALWLLALEPVSETTADHNSYGFRPMRS
                     THDAIESIFLRMSQKVSPKWILEGDIKGCFDNISH"
     gene            2441..4342
                     /gene="ltrA"
                     /locus_tag="OVW45_004272"
     CDS             2441..4342
                     /gene="ltrA"
                     /locus_tag="OVW45_004272"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCV5528564.1"
                     /translation="MLPDELEKRLDGIEDASKKGYPVRNLYKIACEPGLWLQAYVNIR
                     ANAGALTQGVSTDTIDGFSIERAEKLAKTLRSREYVAKPVRRVQIPKKDGKTRPLGIP
                     GGDDKLAQEVARIILERVYEPVFSEHSHGFRPKRSCETALRSIRPVWNGVKWIVDVDI
                     KGFFDNIPHKLLLNTLAEKIKDKNFLKLIEQWLKAGYVDNWKYHRSYSGTPQGGIISP
                     LLANIYLDKLDRFVEQNLIPAYTSGTKRRANPDMNRLAHKIHKLRKKVDGMATDSESE
                     KEAIKKEIERLLEEKRSIPSQVMNDPNFRRMYYVRYADDFVIGVIGSKKDAEHISRQV
                     RNFITTSLGLEVNEAKTRIRHISEGVNFLGYEIRQADAKKLLKQKMQGRHALRRSTTG
                     IVQLFVPDNIAAKFCHQKKYGCYENVKAVHRSSLQNLSEAEIVLTFNAEMRGLANYYS
                     LALDMKYKLSKLYFIWQISLFKTLANKRRSSVNKVAKSLRQNNGDLAITVQAKNGSRK
                     IEVFKLKHVNRNRTTIVDTEPRTAHITTRTTEIMRRLGARICEYCAKTGHCEVHHVRK
                     LKDLKKGRKAGYKPSLWQLMMIARRRKTMILCASCHDKLHSGKLPDLRQEKGRILLQP
                     PVLSGDSAK"
BASE COUNT         1185 a         1063 c         1121 g         1036 t
ORIGIN      
        1 atggctgatg ttatcgaaac accctttaat atcgccctcc agtatccatt tgggcgatac
       61 tttctggctc atccgcagaa aaatggactc aatggcgtca tgtgtactgc gcattggtct
      121 gaaaccatag ctgttatggt cagcagtggt ctcagatacg ggttccagag ccagcagcca
      181 cagtgcctgc atggcacggt ccctcattgt gggtatgccc agtggtcgcg ttttgccgtt
      241 tgattttggg atatgtattc gccgtaatgg ctgtggcctg taaccacgca ggttcaggct
      301 gcatacccct ccccatttct ccttcggtgt gtgccatatt tttccgtcaa taccgggtgt
      361 ccttttaccg gtattttcag ttacacgcct gacagccaca gctttgcctg agaatgagcg
      421 ggtgaggatg cgctgtagtt ctctgacctg tcgccattgt tgctgtcggg ttgcctttgc
      481 aatccttaac tgtagctttc gcacccgggc atggcatgct ttccagttta tggcgtgcca
      541 gcctgtcagg tggggtatcg tggacacact gatgtgtgta ttcatctcgc tgatatcctt
      601 ttgattgctt tacagctttt catgcaatta aagaccagac ggaagtctgc ccgctttcgc
      661 gtcggggaac ctgccccgta tcccggtcat tacagccggg cattcgcttt ttccgtcctc
      721 ctctgcccgc accgccgaca gtgttcctta cggttcacct gcctgtgagg cagcgatacg
      781 ggtttaccag gttccgctta acatacagag ggtcagggcg ggttctgtct cttcaccggg
      841 gggctctgcg cccatgtgct ccctgtgcgc gaggtagcaa ccggaccccg taccttttgg
      901 ttcaggcctg tcagcatctt tggcctgttg ccgataacgg tgtttatcaa cagttcacat
      961 gtgttaaccc ttctgaccag ccttgctctc ctgccgcatg atgcttgcag caaggtaaag
     1021 cccctcacgg ggctttaccc gggctgacgc ccggctctcg ttgtcccggg agcttcagac
     1081 cccgccgttg ccggcagcgc ctgtcccggt aggctacagc taatgggata gctggtttta
     1141 tcaatgtaaa acaatacgtt aagcgacttc ctgtcgcacg cgcaccttcg gctcccggct
     1201 gcctggcggc atccggtcgt caggcccgac tccgacaggc agctgctggc gcacctgcca
     1261 gtctccgccg aacccgctgg cgcggtttcg ggctgtcgct acggtccgat gcgtgaaaac
     1321 cccgggcttg cagcgaccgt tccggtcgcg ccgcccctgt cgcgccgtga cctgcgctcc
     1381 cggcatcccg gcaaaaggcc gccgggatgt aaggcgcaac gccgtttcgc aaaccgcgct
     1441 tcacgtcgtt gcggcgggtc cctgcccgtt gttgtgtccg gtgccattca cctgtttaag
     1501 cccctgtcgc cgggggcaaa gacagccaca accgtctccg ccagtccagg ggtgaaatgc
     1561 acatcccgca aaactttttg cccagaagca ggcaaaaact tttgcggtct gcccccggac
     1621 agtcgtttcc ggccgtggca gtgatgcccc ttgcggcgaa ggttctcaaa cgggtgatgg
     1681 ttagtaccgg agggaacaaa cggcaggtcc ccgcaaaacg tcaaaaccgc ggctcccgaa
     1741 actcaacggg tgatgggggc tgaaaacatc aacagaagga gacatatcat ggcagttcgt
     1801 ggcattaaca aggtcattct agtaggcgtg cgccgttgaa gcgcggtgtt ttatggagtg
     1861 ttgcatgaaa agcaacatgg cttagtggta tagccattgc atactgtggt tagcctcgct
     1921 tggtaacagg ctcggagatc ctgaaaagga gaggtgaaca tagcatccac agaaaagggt
     1981 ggtgaatacg gaaatcgtta taagtatcac cactcaatgc aagatgtata tggataaact
     2041 gggtttgttg aagtcgagat ctatgtggga aacaggaaac caataacgca gaacgattga
     2101 agcgttattc ccgtaatagc acaagaaatc cgccctacgt tgattgtgac cactctaacg
     2161 ggccagctat tgatgaaata gttaaacgat cgaaagacat cctaagtaca tcgcatctcc
     2221 acaccgtgaa actacgggac tacctaaact ggatttatac ccagcatggt agcggagtct
     2281 ccatagtact caaggtcggc ctgtaatggg ctacggcagt ccgcattata cggaagcagc
     2341 aaaggtataa atcaaccgga agggagatga aagcctcaca gtgctgcggg gaagggagac
     2401 agttgttaga tacatcaatc tctaagagga gggttagcca atgctgcctg atgaacttga
     2461 gaaaagatta gatggaattg aggatgcttc aaaaaaaggg tatccagtac gtaatctata
     2521 caaaattgcg tgtgagcccg gactgtggct gcaagcctac gtgaatatac gggccaatgc
     2581 aggtgcactg actcaaggtg tatcaacaga taccattgat ggcttctcta tcgaaagagc
     2641 agagaagctg gcaaaaacat tgcggagtag agaatacgta gcgaaacctg tacgcagagt
     2701 tcagatccca aaaaaggacg gaaaaaccag accgctgggg attccaggtg gagacgataa
     2761 actggcgcaa gaagtagcaa gaattattct ggaaagggtt tatgaaccag tattctctga
     2821 acactctcat ggattcagac caaaacgttc gtgtgaaact gcgctcagat ctattcgtcc
     2881 agtctggaac ggagttaaat ggatcgtgga tgttgatatc aaaggtttct ttgacaatat
     2941 cccccacaaa ctattgttga atacactggc agagaaaatt aaggataaga atttcctgaa
     3001 attaatcgaa cagtggctga aggcaggata tgtggacaat tggaaatacc atcgctcata
     3061 ttctggcacg ccacaaggtg ggataatttc tcccttactg gcaaatatat acctcgacaa
     3121 actggaccgt tttgtagaac agaacttaat tcctgcatat acaagcggta caaaacgaag
     3181 agcgaatcca gatatgaaca ggctggccca taagattcat aaactacgaa agaaagtaga
     3241 cggtatggca actgattctg aatcagaaaa agaagccata aagaaagaaa ttgaacgact
     3301 gcttgaagaa aaacgttcta taccatctca ggtcatgaac gatccaaact tcaggcgtat
     3361 gtactatgtt cgatatgctg atgacttcgt aatcggtgtc atcggctcga agaaagatgc
     3421 tgaacatata tcaagacagg tcagaaactt tattactact tcactgggat tggaagttaa
     3481 tgaagcaaag accaggatcc gccatatctc agaaggagtt aattttttag gctatgaaat
     3541 cagacaggca gatgcaaaga aattgctgaa gcagaaaatg caagggcggc acgctctccg
     3601 gagatccacc acaggaattg tgcagctctt cgttcctgac aatatagccg ctaaattttg
     3661 tcaccaaaag aaatatggct gttatgagaa tgtgaaggct gtacatcgtt caagtctaca
     3721 aaatctcagt gaagctgaaa ttgttctgac tttcaacgcg gaaatgcgcg gattggcgaa
     3781 ctactacagt ctggcgctgg acatgaaata taaattatca aaattatatt tcatatggca
     3841 aataagcctt tttaagaccc tagcgaataa acgacgtagt tcagtgaata aagtcgccaa
     3901 atcgctacgg caaaataatg gagatctggc tattaccgtt caggccaaaa atggatcccg
     3961 aaagattgag gttttcaaac tcaaacatgt caatcgcaac cgaacaacga ttgtcgatac
     4021 ggaaccgaga accgcacata tcacaacccg cacaacagag atcatgcgtc gattaggggc
     4081 aaggatctgc gagtattgtg cgaagactgg gcactgcgaa gtacatcatg tcagaaagct
     4141 aaaagacctg aagaaaggtc gtaaggccgg atataaaccc agcctgtggc aactaatgat
     4201 gatcgcacgc cgccggaaaa cgatgatttt gtgcgcaagt tgccacgaca agttacatag
     4261 cggaaaacta cccgatttga gacaggaaaa gggaagaata ctcctgcaac cacctgtctt
     4321 aagcggggat tctgctaaat gagtttatcc ccaataagct agcaatggag agccgtatgc
     4381 attgaaaggt gcacgtacgg ttcgg
//
LOCUS       DAKISA010000205         4357 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.208, whole genome shotgun sequence.
ACCESSION   DAKISA010000205 DAKISA010000000
VERSION     DAKISA010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4357
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.208"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            331..939
                     /locus_tag="OVW45_004273"
     CDS             331..939
                     /locus_tag="OVW45_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCV5528565.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1037..2428
                     /gene="selA"
                     /locus_tag="OVW45_004274"
     CDS             1037..2428
                     /gene="selA"
                     /locus_tag="OVW45_004274"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCV5528566.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPMIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2425..4269
                     /gene="selB"
                     /locus_tag="OVW45_004275"
     CDS             2425..4269
                     /gene="selB"
                     /locus_tag="OVW45_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCV5528567.1"
                     /translation="MIIATAGHVDHGKTTLLQVITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSVRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKEGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT          988 a         1100 c         1265 g         1004 t
ORIGIN      
        1 cacaccggtg ggggcaccga tgcgcacccc ggctgaaccc tgaacaatgc taccgccata
       61 ctgcgtcatg tcgccctgac gcgctgccgg ttttccgctc atctgctcat ccttgtttta
      121 tcgggttatt tgtctgcttc caggcaaaac cctacagatt tataagaaag ccttaagtga
      181 gatatccatc agatagaaac aattaggaaa atatttatat caccatgata aatatgtgat
      241 ttatttggaa gtaacaaacc atcaacattt tctgcccttt ttcccatcac caggccatac
      301 tgatattttt cctcgcacaa aggactcggc atgaaactcg tcggtagcta caccagcccg
      361 tttgtacgca aactttctat tctgttgtta gaaaagggca taactttcga atttattaat
      421 gaactgccct ataacgcgga caacggcgtg gcgcaattta acccgttagg aaaagtgccg
      481 gtgctggtga ccgaagaggg cgaatgctgg tttgattcgc cgatcatcgc tgaatatatt
      541 gagttaatga atgtcgctcc ggcgatgttg ccgcgcgatc cgctggagtc gttgcgggtg
      601 cgcaaaattg aggcgctggc ggatggcatt atggatgccg ggctggtatc ggtgcgtgaa
      661 caggcgcgtc cggcggcgca gcagtctgaa gatgaattgt tacgccagcg ggagaaaatc
      721 aaccgcagtc tggatgtgct ggaaggatat ctggtcgatg gcacactcaa aaccgatacg
      781 gtcaatctgg cgactatcgc cattgcctgt gctgtcggat atctcaattt ccgccgcgtt
      841 gcgccaggtt ggtgtgtcga ccgcccacac ttagtcaaac tggtcgaaaa cctgtttagc
      901 cgcgaaagtt ttgctcgcac cgaaccgcca aaggcttgat gcgcgatatg tcctcctgac
      961 ccatctcacg ttacaatccg tggttatgtt aaacgccctt ctccgtgtga gagggccttg
     1021 atcagccagg tttcctatga caaccgaaac gcgttccctc tatagtcaac ttccggctat
     1081 tgatcgctta ttgcgcgata gctccttcct ttctttgcgt gatacttatg gtcacacccg
     1141 cgtggtggaa ttgttgcgtc agatgctcga cgaagcgcga gaagtgattc gtgacagcca
     1201 gacgcttcct gcgtggtgtg aaaactgggc gcaagaagtc gatgcccggt tgacgaaaga
     1261 ggcgcagagc gcgctgcgtc cggtgatcaa cctgacggga accgtgctgc ataccaacct
     1321 tggacgagct ttacaggcgg aagccgcggt ggaagccgtt gcgcaggcta tgcgttcgcc
     1381 agtgaccctc gagtacgatc tggacgacgc cggacgcgga catcgcgatc gggcgctggc
     1441 gcaactgctg tgccgtatta cgggggcgga agatgcctgt atcgtcaata acaatgcggc
     1501 ggcggtgtta ttgatgttgg cggccactgc cagcgggaaa gaggtggtag tatctcgcgg
     1561 cgaattggtg gagattggcg gcgcgtttcg tattcccgat gttatgcgtc aggcaggctg
     1621 caccctacac gaagtaggga ccaccaaccg cacgcacgcg aatgattatc gtcaggcggt
     1681 gaatgaaaat accgcactgt tgatgaaagt acataccagt aactacagca ttcaggggtt
     1741 caccaaagcg atagatgaag cggaactggt ggcgctcggc aaagagctgg atgttcccgt
     1801 agtgactgat ttaggcagtg gctcgctggt cgatcttagc cagtacggtt tgccgaaaga
     1861 gccaatgccg caggagttga ttgcggcggg cgtcagtctg gtgagcttct ccggcgacaa
     1921 gttgttaggc gggccgcagg cagggattat cgttggtaaa aaagagatga tcgcccgcct
     1981 gcaaagccac ccgctgaagc gtgcattacg cgcggataaa atgaccctcg cggcgctgga
     2041 agccacgttg cgtctttatt tacaccctga agctctgagt gaaaaattac cgaccctgcg
     2101 cctgcttacc cgcagcgcag aggtcattca aatccaggca caacgtttac aggctcccct
     2161 tgccgcacat tacggcgcgg agtttgcggt acaggttatg ccatgtcttt cgcagattgg
     2221 cagtggttcg ctgccggttg atcgcctgcc gagcgcggca ttaacgttta caccccatga
     2281 tggacgcggt agccaccttg agtcattagc cgcccgctgg cgtgaattgc cagtgccgat
     2341 gattggtcgt atttatgacg gacgattgtg gctggattta cgctgccttg aagatgagca
     2401 acggtttttg gagatgttgt tgaaatgatt attgcgactg ccggacacgt tgaccacggc
     2461 aaaacaacct tattgcaggt gattactggc gtaaatgctg accgtctgcc ggaagaaaaa
     2521 aagcgcggca tgaccatcga tctcggctat gcctactggc cgcagccgga tggtcgcgtg
     2581 cctggtttta tcgacgtacc cggtcatgaa aagtttcttt ccaacatgct ggcgggcgtt
     2641 ggtggtatcg atcacgcgct gttggtggtg gcgtgcgatg acggcgtgat ggcgcagacc
     2701 cgtgagcatc tggcgatttt gcagctgacc ggtaacccga tgctgacagt ggcgctgacc
     2761 aaagccgatc gcgtggacga agcgcgtgtt gatgaggttg aacgccaggt aaaggaggtg
     2821 ctgcgggaat acggttttgc tgaggcaaaa ctgtttatca ccgcagcaac cgaaggtcgg
     2881 ggaattgatg ccctgcgcga gcatctgctt cagttaccag gacgtgaaca cgccagccaa
     2941 catagtgtcc gcctcgcgat cgaccgcgca tttaccgtaa aaggtgccgg gctggtcgtc
     3001 accggtacgg cgttaagcgg ggaagtgaag gtaggcgatt cactctggct gactggtgta
     3061 aataaaccga tgcgtgtacg tgcgctgcat gcacaaaacc agccaacaga aaccgcccat
     3121 gccgggcagc gtatcgcgct taacatcgcg ggtgatgcgg aaaaagagca gattaaccgt
     3181 ggcgactggc tgcttgccga tgcgccgcca gagccgttca cacgggtgat tgtcgagctt
     3241 caaacccata cgccgctgac ccagtggcag ccgctgcata ttcaccacgc cgccagccac
     3301 gttacgggac gcgtttcgct gctggaagat aaccttgccg agctggtgtt cgacacgccg
     3361 ctgtggctgg cggataacga tcgcttagtg ctgcgcgata tctcggcccg caacacgctg
     3421 gcgggggcgc gtgtggtgat gcttaacccg ccgcgtcgcg gtaaacgtaa gccggaatat
     3481 ctgcaatggc tggcgtctct tgcacgggcg cagagcgatg ccgatgcgtt atctgttcat
     3541 ctggaacgcg gcgcggttaa ccttgcggat ttcgcctggg cgcgccagct caacggcgaa
     3601 gggatgcgcg aattgctgca acagcctggc tatattcagg ctggttatag cttgttgaat
     3661 gcgccggttg ccgcccgctg gcagcggaaa attctcgaca cattagcgac ttatcatgag
     3721 caacatcgcg atgaacctgg tcctgggcgc gaacgtctgc gacgtatggc gttgccaatg
     3781 gaagatgaag cgctggtact gttgctgatt gaaaagatgc gcgaaagcgg cgacatcctc
     3841 agtcatcacg gttggctgca tctgccggat cacaaagagg gcttcagcga agagcagcag
     3901 gccatctggc aaaaagcaga gccgctgttt ggtgacgagc cgtggtgggt gcgtgatctg
     3961 gcaaaagaga cgggtaccga cgagcaggca atgcgcctga cgctacgcca ggcggcgcag
     4021 caaggaataa ttaccgcgat cgtcaaagat cgttattacc gtaacgatcg gattgtcgaa
     4081 tttgccaata tgatccgcga tctcgatcag gagtgtggtt caacctgcgc ggcggatttc
     4141 cgcgatcgct taggcgtagg ccgaaagctg gcaattcaga ttctggaata ttttgaccgc
     4201 attggcttta cgcgtcgtcg tggaaatgat catttattgc gcgacgcatt attatttccg
     4261 gaaaaataag gaaatgatta attaattttt aaaataaatt aatacaaatt tctggtaatg
     4321 actccaactt attgatagtg ttttatgttc agataat
//
LOCUS       DAKISA010000206         4332 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.209, whole genome shotgun sequence.
ACCESSION   DAKISA010000206 DAKISA010000000
VERSION     DAKISA010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.209"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            73..387
                     /locus_tag="OVW45_004276"
     CDS             73..387
                     /locus_tag="OVW45_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000600452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine transporter"
                     /protein_id="HCV5528568.1"
                     /translation="MILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILG
                     GLSPLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSH
                     EN"
     gene            377..1264
                     /locus_tag="OVW45_004277"
     CDS             377..1264
                     /locus_tag="OVW45_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCV5528569.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            1405..2004
                     /gene="yihX"
                     /locus_tag="OVW45_004278"
     CDS             1405..2004
                     /gene="yihX"
                     /locus_tag="OVW45_004278"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCV5528570.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            1998..2870
                     /locus_tag="OVW45_004279"
     CDS             1998..2870
                     /locus_tag="OVW45_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCV5528571.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLFSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            2867..3304
                     /gene="dtd"
                     /locus_tag="OVW45_004280"
     CDS             2867..3304
                     /gene="dtd"
                     /locus_tag="OVW45_004280"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCV5528572.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            3349..4290
                     /gene="fabY"
                     /locus_tag="OVW45_004281"
                     /gene_synonym="yiiD"
     CDS             3349..4290
                     /gene="fabY"
                     /locus_tag="OVW45_004281"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCV5528573.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
BASE COUNT         1002 a         1075 c         1157 g         1098 t
ORIGIN      
        1 ttttgtgtcc tgttcgcgtt tcctttcttc ttattactgg atagcaaaag tacgctcatc
       61 atctggtgca gtatgatcct cggctacaac ttaggtccga cgatgatgtt tgccgtgcag
      121 ccaacacttt tcacccgcat gttcggtacc aaagtacgat ataccggcct ttcttttgct
      181 tatcaatttt ccgcgattct tggcggacta agccccctta tcgcctcaag tttgctggcg
      241 ctagggggag gaaaaccgtg gtatgtcgct ctgtttttat ttgctgtctc agttctttcc
      301 tttgtttgcg tctggctgat tgagccgacc gatgaacaag aaaccgcttc ttaccgctac
      361 atcagggaac aatctcatga aaactgaacg aaaaaacgag cattacctgg cgctacaaca
      421 ggcgtttgac gcgccatggc ctgggcctgt aggagagtta gtgacgctgg aaaaaggcaa
      481 tattcacctg cagatttatc cgcatgatgg tgccaggata acctcgttaa aggcatttgg
      541 ttctgaagta ttacgccaat ggcagccgca gcgcagagct tttcagtatg gctgtttccc
      601 aatggtgccg tgggcagggc gactggggaa tgcgacgctg aatgctggtg ggcaatgtta
      661 ttctttaccc gccaataaac caccacatgc gttgcatggt atggcttgtt actcaacgtg
      721 ggaaatcatc gacaaaacag cagattccct gactttacgt atgcccctgg cttcaccgtg
      781 gccctggcaa ggggaagtga ttcagacttt tttgctggag aacgatgcgc tggttttgca
      841 actggaagtc cattcatatg ccgatacatt cccggcctcc gcaggctggc atccgtggtt
      901 tgcgaaaaag ctgactccgc aaaatacaga atcgttgcag gtgctttttg atgcggactg
      961 gcaagaagaa gcgggtagtg atgaactgcc aactggaaat cgtatctctc ctcaagccgg
     1021 tccctgggat gactgttttg gtttttataa cggggtgaag gttaagttac tgtggcctgg
     1081 taaactcgcg atgaccatga tctcttctgc aaacagtctg gtggtatttg ataaacagcc
     1141 cgatgccacc tgtattaacc cgctgactca ggcacctaac gcaataaatc taacgccgga
     1201 attggtgaca ccagataggc cgctggtcat cgaaactcgt tggcaattta cacctgaatc
     1261 ttaaccattc atctattgcg aaaaagccag tgaaattact ctgctttttc gcaatcagca
     1321 tcacaagatt acccttattg ccacatagcc aaacccatcc tttcccgcta cagttaattt
     1381 cttgtggcgc gaaaggaggc aaaaatgctc tatatctttg atttaggtaa tgtgattgtc
     1441 gatatcgact ttaaccgtgt gctgggagcc tggagcgatt taacgcgtat tccgctggca
     1501 tcgcttaaga agagttttca tatgggggag gcgtttcatc agcatgagcg tggggaaatt
     1561 agcgacgaag cgttcgcaga ggcgctgtgt catgagatgg ctctaccgct aagctacgag
     1621 cagttctctc acggctggca ggcggtgttt gttgcgctgc gcccggaagt gatcgccatc
     1681 atgcataaac tgcgtgagca ggggcatcgc gtggtggtgc tttccaatac caaccgcctg
     1741 cataccacct tctggccgga agaatacccg gaaattcgtg atgctgctga ccatatctat
     1801 ctgtcgcaag atctggggat gcgcaaacct gaagcacgaa tttaccagca tgttttgcag
     1861 gcggaaggtt tttcacccag cgatacggtc tttttcgacg ataacgccga taatatagaa
     1921 ggagccaatc agctgggcat taccagcatt ctggtgaaag ataaaaccac catcccggac
     1981 tatttcgcga aggtgttatg ctaaaaacca ttcaggacaa agccaggcac cgtacccgtc
     2041 cactatgggc ctggctaaaa ctactctggc aacgtattga tgaggacaac atgacaaccc
     2101 tggcaggtaa ccttgcctat gtgtctttgc tctcattagt gccgctggtt gccgttgttt
     2161 ttgcgctttt cgccgctttt cccatgtttt ccgacgtcag cattcagtta cgtcacttta
     2221 tttttgccaa ctttctgcct gctactggcg atgttattca gcggtatatc gaacaatttg
     2281 ttgccaattc caacaagatg accgccgttg gggcgtgcgg gctgatcgtc accgcgttat
     2341 tgttgatgta ctccatcgat agcgcgttga ataccatctg gcgcagtaaa cgagcgcgac
     2401 caaaaattta ctcgtttgcc gtgtactgga tgatcttaac gctggggccg ctgctggcag
     2461 gggccagtct ggcgatcagt tcctatttgt tctccctgcg ctgggcgagc gatctcaata
     2521 ctgtcatcga taacgtgctg cgtatttttc cgctgctgtt gtcgtggatc tccttctggt
     2581 tgctgtacag cattgttcct accatccgcg tacctaaccg cgacgcgatt gtcggcgcgt
     2641 ttgtcgctgc actcctgttc gaagcaggaa agaaaggttt cgcgctttat atcaccatgt
     2701 tcccgtcata tcagctcatt tacggcgtgc tggcggtgat ccccattctc ttcgtttggg
     2761 tctactggac atggtgtatc gtcttgcttg gcgcggaaat tactgtcact ctcggggaat
     2821 accgcaaact aaaacaagca gctgaacaag aagaagacga cgaaccatga ttgcattaat
     2881 tcaacgcgta acccgtgcca gcgtcaccgt ggagggagaa gtgacgggcg aaattggcgc
     2941 gggacttttg gtgttattgg gtgtcgaaaa ggatgacgac gaacagaaag caaaccgtct
     3001 gtgcgagcgt gtgctcggct accgcatctt tagcgatgcc gaaggcaaga tgaatctcaa
     3061 cgtgcaacag gcgggcggca gtgtactggt ggtttcccag tttaccctcg ccgcagatac
     3121 cgaacggggg atgcgcccaa gtttctccaa aggcgcatca ccggatcgcg cagaggcgtt
     3181 atatgactat ttcgtcgaac gctgccgcca gcaagagatg aacacgcaaa caggacgctt
     3241 cgctgccgat atgcaggtat cgctggtcaa tgatggcccc gtgacattct ggttgcaggt
     3301 atgagcctgc ttccagggtt gtcacgggaa acaagagaga gtatcgctat gtatcacctt
     3361 cgggttccac aaacagaaga agagttagag cgttactatc agtttcgctg ggaaatgttg
     3421 cgtaagcccc tgcatcaacc aaaaggttcg gaacgcgacg cgtgggatgc gatggcgcat
     3481 caccagatgg tcgtcgacga gcagggtaat ctggtggcgg taggccgatt gtatattaat
     3541 gccgacaatg aagcgtccat tcgctttatg gccgttcacc cggacgtgca ggacaaaggg
     3601 ttaggaacgc tgatggcgat gaccctggag tcggtggcgc gtcaggaagg cgttaagcgc
     3661 gtgacctgta gcgcccgtga agacgcggtg gagtttttcg ccaagctggg attcattaat
     3721 cagggggaga tcaccacccc aactaccacg ccgattcgcc attttttgat gattaaaccc
     3781 gttgccactc tggatgacat tctgcatcgc ggtgactggt gcgcgcagct gcaacaggcg
     3841 tggtacgaac atatcccgtt aagcgaaaaa atgggcgtgc gcattcagca atataccggg
     3901 caaaaattta tcaccaccat gccagaaacc ggtaatcaga atccgcacca tacgttgttt
     3961 gccgggagtt tattctcgct ggcgacgctc accggttggg gacttatctg gctgatgctg
     4021 cgtgaacgcc acctcggcgg aacgattatt ctggcggatg cgcatatccg ctacagcaag
     4081 ccgattagcg gtaaacctca tgcggtagcc gaccttggtg ccttaagcgg cgatctcgac
     4141 cgtctggcgc gcggacgaaa agcacgggtg cagatgcaag ttgaaatctt tggcgacgag
     4201 acgccgggtg cagtgtttga aggcacgtat atcgttctgc ccgcgaagcc atttggcccg
     4261 tatgaagagg gcgggaacga agaagagtag cttatggaga ggggcggtgc tgcctctctc
     4321 attcattccg gt
//
LOCUS       DAKISA010000207         4326 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.210, whole genome shotgun sequence.
ACCESSION   DAKISA010000207 DAKISA010000000
VERSION     DAKISA010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.210"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..579)
                     /locus_tag="OVW45_004282"
     CDS             complement(<1..579)
                     /locus_tag="OVW45_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCV5528574.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIDLGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADAITVDAMIPN"
     gene            complement(642..1091)
                     /locus_tag="OVW45_004283"
     CDS             complement(642..1091)
                     /locus_tag="OVW45_004283"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCV5528575.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(1088..4180)
                     /gene="ebgA"
                     /locus_tag="OVW45_004284"
                     /pseudo
     CDS             complement(1088..4180)
                     /gene="ebgA"
                     /locus_tag="OVW45_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
BASE COUNT         1026 a         1090 c         1167 g         1043 t
ORIGIN      
        1 gttcgggatc atcgcgtcaa cggtgatggc gtcagcaggc tgtacgccgc caaccagcgc
       61 cgtacctgcc agtaaaatgt aggagagcgt taacagcagc atcagcgttg aagtgacgga
      121 ggtaattggc cccaacattt tcgccccgtt ggtggaaacc caggtggaga aggcgaacag
      181 caccatactg ataatggtgg tggcaaccgg cgtcataata tattcgtatc cgaggaaggc
      241 atacgaagca taagcaataa cgcgcggcaa cagtgaggtg aaaaagaaca ggtttacgaa
      301 ccagtaggta taggcagtaa taaatgccca acgtccgccc agcgaacttt ttacccatgc
      361 gtagacaccg gcttctgagt ttttatttaa cgaaacaaat tctgcgatga tcagacagaa
      421 gggaataaaa taaaaaatcg tcgcgaggaa aaacatcggt gccgaggcca gtccaagatc
      481 aatattatta ttgataacgt tgttaaagct gtaaacggcg gcaaaagtca gcgagagcaa
      541 gccgaatttg ccgattgtat tacgtttggt atcagacata acacatctcc gaaaatgagt
      601 agggtagcgg tggcggcaaa ctccttttgc cgccgtagtt gttatttgtt atggaaataa
      661 ccatcttcga tggtgacttt gagaaccact tttttgaccg cgttattgca gataaaccga
      721 tacgcttcat ggatatcgca aataacgatt tgcccttcgt ggacctcaac ggtttctccg
      781 cagcctttta aatattcacg gtcagtttca tcacgataat attccactac ctgtaatgtc
      841 tctttcggcg catattcaat tttttgctgc ccttgcaggt agtaatgcac ttcaaaatag
      901 cgtcgatgcc cggtaaatag cgcatcggtc gcggagtctg tctccacgcg gtaagtcaat
      961 gagtcaccga tggagtgggc gacgccaggc tgaatgttgt cgatattttc aatcgcttca
     1021 acgcagcgtt gccacttctt gccagaggcg taaatctggc ggaactgttc taagttatcg
     1081 atgatcctca ttgcttattc tcgctgtgca aattcgtgga aaagaaccct gcgccgaact
     1141 catacgacgc caggctttgc gcggtagctt ctccgccaga aaccggcagc aacgtaaagc
     1201 cgtagctgaa gtcacggaac cagacgcgcc aggagtccag cacctcgctg ccccaggagt
     1261 tggagccgag gccaagcagc tggtgatcga ggttcagggt gatgtcatca ctgcgctgca
     1321 gctcgttaca gtgctgggca gcgtggatgt tttcctaggt atagtgccag gcgctgaagt
     1381 taattgggcg ctgcggaacc accagcagac cgttaccgtg gcggttagtc agtgccgtcc
     1441 agcggacatg ctgacggtta ccgttgttct gcgggaaggg atagttctcg aacatggcat
     1501 cgacggtgct gcgccagata tcgatgatgt tagcctgctg gctgtcggcg tagttttcgc
     1561 ccggtccacg accgtaatac gccacctgat cgtattcgcc gttaattccc atggtgaaac
     1621 cgatgcacgg aatgatgtgc ggatagtcgc cgtaacgctc gccggaaagc gccacgttaa
     1681 cctggccatc ggcagcgatg cgccagatgt aggtgcagcg cattccgaaa tcaaacaccg
     1741 gcggggcaat aaccgtgcgg ctgatgatca acacttcacc ctcgctctgt tctacggcaa
     1801 agtcgcgcag atgttcctgc atgatctgca aatgattcgg ctgccacaac ccttcgtact
     1861 cctgcttgtg gttgtcgatc atcggcttga agaagttgat ctttggctcg cgggtcagca
     1921 gcgactcgcc atttacctgc caggatgtcg gtttgccact catttttgag aaggtgatcg
     1981 cgaagttgta gccgcgaacg gtgcagctca aacgatcgtc ttccagcgtc agcggacgcg
     2041 cattatttgg tgcgaaaggc actggctgcg cggtgttttc cttcagcggg aactgataag
     2101 tggcgataga atgcccggct tcgctgtagc gggtgcggga atctttggtc accgtaatgt
     2161 tgaggaacgc ttcgcgggcg tccagctgcg gcagcgtgat ctgcaagggg gcttcgctgt
     2221 tcggcgcaac gtcgcgcagt ttaatctgct gcgtcgcgag cgtttcacct tcggcgcgca
     2281 cctctgcgtg cagggtgtag tcatcaagcg tggtaaacca cagtttattt tcgactttca
     2341 actcgccgcg agtcagatcc cgcgcgtgga tttttaccgg cgcgataacc tgtttgtact
     2401 ctttcaggcc cggtcccggc gtctgatcgg aatagatcaa accatcaaga cagaagttat
     2461 agttgttggg atagtcgccg tagtcgccgc cgaatttata ccagacattg ccgtggtcgt
     2521 cctgtgcctg gatcccgtgg tcgcaccact cccagacata atgaccctga atgcaatcgt
     2581 gcttatagaa gacgttctgg tactccgtca gcccgcccgg tccgttcccc atcgcatgag
     2641 catattcaca gatgatgcgc ggcttcggat gcgggtattc accaaactca ttcatcagcg
     2701 gcacgcgggt gtacatggtg gaaataatat cgaccacttc agcatcgcga tcttcttcgt
     2761 aatgcaccag tcgcgtgtca tccagcgcct tcgctgcgtg gtacatcgcg cggatgttac
     2821 agccatagcc ggattcattg cccagcgacc agatgatgat cgacggatgg tttttctgcg
     2881 cgtgaatatg gcgaacaatg cgctcgacgt agactttttc ccactgcgga tcgtcggtaa
     2941 tacggctgat atcgccgaca ttagcaaagc cgtgcgattc gacgtcggtt tccgccatca
     3001 caaacaggcc gtagatatca cacagttcgt aaaaacgcgg atcgttcggg tagtgagcgg
     3061 tacgcacgga gttgatgttg tgctgcttca tcaactggag atctttctcg acgcgatcca
     3121 ttccaacggc gcggcctttg cgatgatcgt tgtcgtgacg gttgacgccg tgcagcatca
     3181 cataacggtt attgatccag aacagaccgt cgcgcacttt gatatcacgg aagccaacgc
     3241 gttgtggtac cacttccaga acgttgccgt tggcgtcttt cagcgtcatg accagatggt
     3301 aaagataagg ggattctgct gaccattgct gcggctgttc gacagtaaaa gcaaagctgg
     3361 cgctggtcag tttttcaatt gccaaatgat caatggcgct gctgtgcacc acgcgttcgc
     3421 catcaaacag ggtatattcc agcgtcgtga cgacagggga ggcggcgaga ttttccagca
     3481 ccacttcgca ggaaagcgtg gcatcgcaat aggcttcgtc aaagtcggta cgcacggtga
     3541 aatcgttaat atgtgttagt tgttttccga tcagataaac atcgcggaag atccccgctg
     3601 accaccacat atcctggtct tccacgtagg tagagtccgc ccattgcatc acgcgcacac
     3661 acaacaggtt gtcgccggtt ttaaccatcg cgctgatgtc aaactctgcg gtcaggcgac
     3721 tgcccttgct gaaacccaca tactgaccgt taacgtagac ttcaaaatag gtttcgacgc
     3781 cgtcaaattt aatcagcgtc tgtttaccct gccagccgtc gctgagggtg aaaatacgtt
     3841 gataggcacc ggttgggtta tcgctgggaa caaacggcac atcgatgggg aacggaaaac
     3901 cttcgtcggt atattgcagt ttgccgtgac cttccatttg ccacatggcg gggacggtaa
     3961 tatgccccca gtcagccatt aactcagagg tgaaggcttc aggcacttgc agcgggtggt
     4021 caaaaaagtg gaaattccac tgaccgctta agggcagaaa caggctgctg gtttcgcggg
     4081 caaaggtacg cgcttgcgca acagaatcat atgaaaaaaa gtacgcacgt ggcgcaagtc
     4141 ggttttcgtg ggtgagctga atgttttccc agcgattcat agggcctccc ggtagagaga
     4201 aatcggaacc attcatggtg atggtagttt gtgtaattgc tggggagttt agtaaaattt
     4261 ttactaaaaa gtggcaggta aatgcattta ctttaactgg atcacgaaaa taaacggcag
     4321 cactgt
//
LOCUS       DAKISA010000208         4310 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.211, whole genome shotgun sequence.
ACCESSION   DAKISA010000208 DAKISA010000000
VERSION     DAKISA010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.211"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            184..336
                     /gene="hokA"
                     /locus_tag="OVW45_004285"
     CDS             184..336
                     /gene="hokA"
                     /locus_tag="OVW45_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCV5528576.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACNLKE"
     gene            complement(524..736)
                     /gene="cspA"
                     /locus_tag="OVW45_004286"
     CDS             complement(524..736)
                     /gene="cspA"
                     /locus_tag="OVW45_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCV5528577.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1017..1307)
                     /locus_tag="OVW45_004287"
     CDS             complement(1017..1307)
                     /locus_tag="OVW45_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HCV5528578.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1741..2451
                     /locus_tag="OVW45_004288"
     CDS             1741..2451
                     /locus_tag="OVW45_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCV5528579.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2501..3475)
                     /gene="ghrB"
                     /locus_tag="OVW45_004289"
     CDS             complement(2501..3475)
                     /gene="ghrB"
                     /locus_tag="OVW45_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCV5528580.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLHATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3579..4238)
                     /locus_tag="OVW45_004290"
     CDS             complement(3579..4238)
                     /locus_tag="OVW45_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCV5528581.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLTEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
BASE COUNT         1073 a         1036 c         1051 g         1150 t
ORIGIN      
        1 agaagaaaca aacttattaa gctagaatag ccacgggtgc ttgagactgt ttgtctcagg
       61 tattcaccga aaggcagaca gagaaaagcc ccacctgact ataaatcaaa gtgaggctac
      121 cctatgcctg aacaccataa ggttagcctc ttactcgttg gaaatcaaca caggaggctg
      181 ggaatgccgc agaaatatag attactttct ttaatagtga tttgtttcac gcttttattt
      241 ttcacctgga tgataagaga ttcactgtgt gaattgcata ttaaacagga gagttatgag
      301 ctggcggcat ttttagcctg caatttgaaa gagtaagagt ctttggcggg aagttattcc
      361 cgcctttctt acggcgttgc gcattctcat tgcacccaaa tttattcttc acaaaaataa
      421 tactaaattt tattacgcga tcgattattt atttcctgaa aaacaaataa aaaaatcccc
      481 gccaaatggc agggatctta gattctgtgc ttttaagcag agattacagg ctggttacgt
      541 taccagctgc cgggccttta gcgccgcttt cgatggtgaa ggacactttc tgaccttcgt
      601 ccagagattt gtaaccatcg ttctggatag cagagaagtg tacgaacaca tctttagagc
      661 catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt gaaccatttt acgataccag
      721 tcattttacc ggacatagtg tattaccttt aataattaag tgtgcctttc ggcgatatgg
      781 cgtgctttac agattttgaa gcgttaaagg aatgtgcact acgaggggta tcaacgataa
      841 ctcttgaagg gacttgcctt actacactgc tttaatggtc tgtacgtcaa accgttgatg
      901 tgcattaagc cacgcattgg cgggtgatgc aacaattatt tttcatattt atgattaatc
      961 ggttggaaaa cggtcctgtc atcaggaccg taaacagcaa taaagtggat aaaagtctat
     1021 tccatcaact gcttacttaa tgccgggttg gcttgaatca aacgcatcaa ttttagttcg
     1081 gcacttgaag gcttcacgcg tctggattcc cattccttta ccatggcgac tgatacgccc
     1141 aaaacccggg cgaaatcatc gatttttaac cctgtccctt ttcgtaactg ctcaatttcg
     1201 gtacaggacg ttgttctgtg cgtcagggta attttctgcg tttcatcttt aaaaacaatc
     1261 tgttccaggc tgctcaacag ctcatgcatt ggatctttat attccattga aaactcctca
     1321 aatcacactg cgggatcgtg aattgcatcg aagctcatta agaatagtcg ggaaaagcag
     1381 acacagcggg gtgaagtcag ggcatgctct ggctatgatt cgattcagcc tgtcatttag
     1441 cgtgacttat agccacctaa cgatctgata aatgccgata tatctcttac cactcctgtt
     1501 ttgtaaagat ttcgaaaatc taaccttctg cgaaggcgta aatctctggc tacaaaaaag
     1561 gcaacggcac tatcattttt taacaatgaa acaacaaatt aaccttgttt cattttctcc
     1621 atgattttac caggaagatt acgtagaaag ggtatcctgc aagcctgtcc tggactatcc
     1681 ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt tgggtgaaag gagtaagaaa
     1741 atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc ttgcggcgtt attaatggta
     1801 gttagcctga gtgggtgttt tgataaagaa ggcgatcagc gtaaagcgtt tatcgacttc
     1861 ctgcagaata cagtgatgcg tagcggtgaa cgtctaccaa ccctgactgc cgatcagaaa
     1921 aaacagtttg gtccttttgt ctctgattac gcgattctgt atggttattc tcagcaggtg
     1981 aatcaggcga tggattctgg tctgcgcccg gtcgtagaca gcgttaacgc aattcgcgta
     2041 ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa tgaatggttc gctgggtgtg
     2101 ctggcgcaac aactgcaaaa tgcgaagttg caggccgatg ctgcgcactc tgcgctaaaa
     2161 cagagcgatg acctgaaacc ggtatttgat caggcattca ccaaagtggt aacgacgcca
     2221 gctgatgcat tgcaaccgtt gattccggcg gcacaaactt tcacgcaaca actggtgatg
     2281 gttggagact acattgctca gcaaggtact caggtgagct ttgtcgcaaa tggcatccag
     2341 ttcccgactt cacagcaggc aagtgaatat aacaaactga ttgcgccatt accggcacaa
     2401 catcaggcgt ttaatcaggc ctggactaca gcagttactg caacccaata aagagtaaaa
     2461 gcccgagcga ttatcgggct ttacttcacg cagtcgcggc ttagtccgcg acgtgaggat
     2521 tcacacagtt cttctcaacc tttccttgta acgcatcaat taaattatcc acggcacagg
     2581 cggccatgcc ataacgcgtc tcatgggtgg cagatccaat atgcggtact gcgacgacgt
     2641 tggccattga gagcaacggc gaatctacgg acagtggctc ttgttcgaag acatcaagcc
     2701 cggcggcgtg aatttcccct ttctgtaatg ctgcgatcag tgcattttcg tcaaccaccg
     2761 ggccacgtcc ggcattaatg aaaatggcgg aggatttcat tttggcgaat tgttctgcgc
     2821 caaacagatg atgcgtctca tcagttaacg gcaggatcag gcaaacgaaa tctgactctt
     2881 gtaacagtgt atccaaatcg cagtagcggg cgttgaagcg ttcttctgct tctttatggt
     2941 ggcggcgcgc gttatagagg atgggcatgt tgaagccaaa gtgcgcacgt tgtgccagcg
     3001 ccataccgat ccgtcccatc ccgacaatgc ccagtgtttt atggtgaacg tcagtgccgt
     3061 accagtccgg gcctatgctc gcggtccatt cgcctgcttt tacccgttct gctacctcca
     3121 caacccgacg agcggtagac aacaccagcg ccatcagcgt atcggcgacg gtttctgtta
     3181 atacggttgg cgtgtgcatc agcagaattt ttcgggcggt aagcgcatcg acatcaaaat
     3241 tgtcatagcc gacggagatc gttgatgtgg catgcagttt cggcattttt tccagcaatg
     3301 cggcatcaac attctcgttt gaacccagta aaccttcagc ttcggcaaaa attgctgcat
     3361 tttgttcgac ggtttgtggg ctgaggtttg ccacctggtg aacggtgaaa tgctcttgca
     3421 ggcgttgcag taaatcatca ggtaaggctt tgtagaggat aacggacggc ttcatgcttc
     3481 tctccattca ctgataaggc gatcacctta gcctgaaata tgatgaagga aaactgaaga
     3541 atgccaggtg atttctgcat cacctggcat gaaagggatt acagcgggct taaggtaatt
     3601 tctacacggc ggttttgcgc cttaccttct gcggtgctgt tgctggcgat tgggttagcc
     3661 gggccaaggc cctgagtacg gatgcggctg gcgtccacgc cctgggtgat caacgcgctg
     3721 gcaacggaat ccgcacgctg ctgggagaga cgcatgttca ggtcgtgacc ccccgtgctg
     3781 tcggtataac caatcacgtt aaccgccgtt ttcggatact ctgtcagcac cattgccacg
     3841 ccggtcaggg tgttagcgcc tgccggtttc agggtcgcgc tgctgctgtc gaaggtcaca
     3901 ttgttcggca tattgaggat aatgttatcc ccgctgcggg ttacgctaac accagtgccg
     3961 cgcattttgt cacgcagctt cgcttcctgc acatccatgt aataacccac gccgccgccc
     4021 agagctgcgc ctgctgctgc gccaatcagc gcgcctttac cgcgatcttt cttcgaagaa
     4081 gagagcgcac caatacccgc gcccacgaga gagcccagac ctgcgccgat agcagatttg
     4141 cctgcttcgc gttcgccggt gtaagggtta gttgtgcagc cagataccgc cagagcgcca
     4201 ctcactacgg cggcaataag ataaacacgt ttcttcattg ttaatcctta ataacctttt
     4261 tattctttgc cacgggttcc gtggcgggag attatgccgc gtgaacatga
//
LOCUS       DAKISA010000209         4309 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.212, whole genome shotgun sequence.
ACCESSION   DAKISA010000209 DAKISA010000000
VERSION     DAKISA010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.212"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..1870)
                     /gene="speC"
                     /locus_tag="OVW45_004291"
     CDS             complement(<1..1870)
                     /gene="speC"
                     /locus_tag="OVW45_004291"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCV5528582.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATSVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLHQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMTPQDA"
     gene            2269..2976
                     /locus_tag="OVW45_004292"
     CDS             2269..2976
                     /locus_tag="OVW45_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCV5528583.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3082..3157
                     /locus_tag="OVW45_004293"
     tRNA            3082..3157
                     /locus_tag="OVW45_004293"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3115..3117,aa:Phe,seq:gaa)
     gene            3355..>4309
                     /locus_tag="OVW45_004294"
     CDS             3355..>4309
                     /locus_tag="OVW45_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCV5528584.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSNKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPI"
BASE COUNT         1114 a         1141 c         1038 g         1016 t
ORIGIN      
        1 gcgcatcctg gggggtcatc accactgacg ggaaactctg ctggcggaac atcgcttttt
       61 gtaggtcttt gacgtcgaaa ctgacataca gatcgtgcat ctcctgacac aactggtgca
      121 gggtgtagtc gcgatagcgc accggatatt tgttataaac gctcggcaac acctcagcca
      181 gcggcgagtc atcctcaata tgctgttcaa attgcgccag catcgccacc agttgtgcca
      241 gcttctcgtg gctttccgcc ggagttaata agaacagaat ggagttgaga tcgcacttct
      301 ccggcacaat gccgttctca cgcagatagt gcgccagaat cgtcgccgga acgccaaagt
      361 cgctatattc gccggtttcg gcattgatac ctggtgtagt gagtaacagc ttgcacggat
      421 caacaaaata ctgatccgcg gcatatcctt caaagccgtg ccacttcgcc cccggctcaa
      481 aactgaaaaa acggcggtcg ctggctaaca cagatgtcgg ataatcctgc cacaatttgc
      541 catcaacaac gggcgggata aacgggcgga acagcttaca gcgcgcaaga atagccttgc
      601 gcgactcaat acccaactca acgcactcag cccacagccg acgcccactc tccccttcat
      661 gaattttggc gttaacatcc agtgcagcaa acagcggata gaaagggctg gtagaagcat
      721 ggagcataaa ggcgttattc aaccgcttat gcgggcaaaa acgcgcctgt ccgcggatat
      781 ggttatcttt tttatggatc tgcgacgtct gtgagaatcc cgcctgctgt ttgtgcaccg
      841 actgagtcac aaagatcccc ggatcgtttt cgttaagttc taatagcagc ggcgagctat
      901 ccgccatcat cgggataaac tgttcgtaac cgacccacgc ggaatcaaac agaatgtaat
      961 cacacagatg cccaacggta tcgatcacct gacgggcgtt atagacagtt ccgtcatagg
     1021 ttcccagctg aataatcgcc aggcgaaacg ggcgcggcag ctcggctttt tctggcgcaa
     1081 cgtcgcgaat ttgctggcgc agatactctt cattaaaaca gtgcgcatca ataccgccaa
     1141 tgaaaccaaa cgggttgcgt gaagcttcca gatagaccga cgtcgccccc gcctgaatca
     1201 gcgcgccgtg atgattcgac ttatggttgt tacggtcgaa gagcaccaga tcgccacgcg
     1261 ttaacagcgc attcgtcacc actttattcg ctgccgatgt gccgttcagc acaaaatagg
     1321 ttttatcggc atgaaagact ttggctgcga atttctgcgc atccttcgcc gatccttcat
     1381 gaataagcag atcgcccaat tttacgtcag cgttacacat atcggcgcga aagacgttct
     1441 caccaaagaa atcgtaaaaa tggcgtccgg caggatgctt tttaaaaaac gcaccatgtt
     1501 gatgtccagg gcaagcaaag gtgctgttgc ccatctcaac gtactgcgtc agcgtgtcat
     1561 aaaacggtgg cagcaaattc tcttcatact gacaggctgc ggattccagc tccagccact
     1621 gctgctcgtt gccgttgatt accgccgtaa cgcccgcagg taattcaaca gcatgttcgg
     1681 aatacaaaaa caccggtaga tgaaaaccgg tgcgcttaag caacgcaaga atgccactgc
     1741 gactatccgc agcggtaatg acgactgccg cgacatccgt aaaatcagta tctcccaacg
     1801 ccaccacgcg gcgatgagta gaaagtcggg ataccagttc actactggcg gcaatattca
     1861 ttgatttcat aagcgcaaaa cccgtttcgg ggaagtaaga ataccggaca aagtgtaaaa
     1921 ccctgcccca tgagatatgg gtcaaactgg tcaccagctc cgaccgccag acatcagtaa
     1981 aagcagaaac gctctgattt tactgttgtc ctgcagtgaa cgtgcgttaa cttcaccgca
     2041 tgagcagtaa catagaaagg gaaacgtttc gcgcgaaacg gcgataagcg agagaatgta
     2101 aggagatggc gtgcatcggg caaactccgt acagagagga gaaaattcgc gcaatcatgg
     2161 cacctttcgc taaggcgtgc aagccaggac tttgcgacca gacaagccat caacaaggca
     2221 aaaagttcat aataagaaaa tcaaacaata cagctaacag gaactcttgt ggtcatcggc
     2281 ccttttatca acgctagtgc agtcttactg ggtggcgttc tcggcgcact gctcagccaa
     2341 cgcttaccgg aacgtatccg cgtctccatg acatctattt ttggtctggc atcgctgggg
     2401 attggtattt tactggttgt gaaatgcgcc aaccttccag cgatggtttt agctacgcta
     2461 cttggcgctc taattggcga aatttgtttg ctggagaaag gtgtcaatac agcagtcgcc
     2521 aaagcacaaa atctgtttcg tcactcacgt aagaagccag cgcatgaatc ttttattcag
     2581 aattatgtcg cgattattgt cctgttttgc gccagcggca ccgggatctt cggggcgatg
     2641 aacgaaggga tgaccggcga tccgagtatt ttaatcgcca agtcatttct tgatttcttt
     2701 acggcgatga tcttcgcctg ctcgctgggt attgcggtgt cggtaattag tatcccatta
     2761 ctgatcatcc agctaacgct agcgtgggct gccgcgctga tattaccgct gaccacaccg
     2821 tcgatgatgg cagacttcag cgccgtaggc ggtttattgc tgctggcaac cggattacgc
     2881 atctgtggca ttaaaatgtt cccggtggtc aacatgcttc cagcactcct tctggcaatg
     2941 ccgctttccg ccgcctggac cgcctggttt gcctgacaat gcgtgcaata gcggcaaagt
     3001 gatgatagat tgtgcagtct gcagtaaatt gaagaaattt gattgacgag acgaggcgaa
     3061 tcaggtttaa tgcgccccgt tgcccggata gctcagtcgg tagagcaggg gattgaaaat
     3121 ccccgtgtcc ttggttcgat tccgagtccg ggcaccaaat tcatatcaac ggacctccac
     3181 ggaggtccgt ttttcgtttc agaacgccac gatttaagcg ttctgcctac aaatcaattc
     3241 taccgaactc aaccagattc cccccacatc aaccccattg tgtgggtata attgcgggca
     3301 taccccagtt cgacagaatt tgtaccctct ttaacgcatt cagaaggctg aactatggca
     3361 ctgactgacg caaaaatccg ggctgcaaag cccactgaca aggcttataa actcactgac
     3421 ggggctggca tgttcctgct ggtacatcct aatggctccc gttactggcg tctccgttat
     3481 cgtattctgg gtaaggagaa gactctggca cttggtgtgt atccagaagt ttctctctcc
     3541 gaagctcgta caaaacggga tgaggcccga aaactgattt cggagggggt tgacccttgc
     3601 gaacagaaaa gagctaaaaa agtagtccct gatttacagc tctcttttga acatattgcg
     3661 cgacgctggc atgccagtaa taaacaatgg gcacaatcac acagcaataa agtactcaaa
     3721 agcctcgaga ctcacgtttt cccctttatc ggcaaccggg atatcacaac actcaatacc
     3781 ccggatctgc ttatccctgt tcgtgctgca gaagctaaac aaatttatga aatcgccagt
     3841 cgtctgcagc aaagaatatc tgccgtaatg cgttatgccg tacagtctgg catcatcaga
     3901 tataatcctg ctctggatat ggctggcgca ttgactacgg taaaacgcca gcatcgcccc
     3961 gctcttgatc tttctcgcct gcctgaactt ttgtcgcgta ttggcagtta caaagggcaa
     4021 cctgtcaccc agcttgccgt tatgctgaat ttactggttt ttattcgttc cagtgaactc
     4081 agatacgccc gctggtctga aattgatatt gacaatgcca tgtggactat tccagccgaa
     4141 cgcgaacccc tgccaggcgt aaaattctca caccggggct ccaagatgcg aacaccacat
     4201 cttgtgccac tcagcaaaca ggctgtagcc atactgacag aactacagac atgggctggt
     4261 gaaaatggtc tgatctttac gggagcacat gacccgcgta aaccaatca
//
LOCUS       DAKISA010000210         4243 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.213, whole genome shotgun sequence.
ACCESSION   DAKISA010000210 DAKISA010000000
VERSION     DAKISA010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.213"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            45..731
                     /locus_tag="OVW45_004295"
     CDS             45..731
                     /locus_tag="OVW45_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016152569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCV5528585.1"
                     /translation="MQLKPLTKGRFHNIWQQNDAFIEKTFRELLSETQLIHEGDYLFR
                     QGQVVDRLIMVETGRISLSYSAENGRCFQFGTMDCDEQLFGEMEFFTGYLCQLDIVAE
                     EPLIVGVCSSERLEAALDQHPKLALFFASAIAVDYQDTLEIFLHRMLYPIAHNIAYDL
                     YRQHQNKQPMDGFSKNYKEAERFGTTDRVYRRAVKTLVDAGLVIRTKQGLEIKNLDEL
                     RRFLGNDSFD"
     gene            complement(785..1621)
                     /locus_tag="OVW45_004296"
     CDS             complement(785..1621)
                     /locus_tag="OVW45_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HCV5528586.1"
                     /translation="MSHTNKMNVKIVLISMAIGMASVNTFAADYADGNIRKNDFNWMQ
                     INLMQSVDAKVPYGIRDDTYLELEFGARSGIVDLYGYVDFFDVFDNKHDDRHGGDNFF
                     AKISPRFSLDAIFNKDLSVGPFNEFYIATVNNIGDRELFEHYVGMGTDVKVPFLGLVG
                     MNVYAHYVRENYGADNEGKWDGYMFSTNWSTPVWNFANGSYLNYQGYFDYQFAANKIA
                     DQALYSNNAIEWYNGIYWHSENYAIGYGLKYFRNMALMQNHGGAGRTTGLGHYFNLTY
                     KF"
     gene            complement(1702..2865)
                     /gene="tsgA"
                     /locus_tag="OVW45_004297"
     CDS             complement(1702..2865)
                     /gene="tsgA"
                     /locus_tag="OVW45_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016149894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCV5528587.1"
                     /translation="MKNKIALTLISFLANFIMAGFASQFGMLIEPIAQRYDADINAVA
                     SIFSLLNGGALAGTIAAFFLIEKIGVKRMTFIIYSLVFLCAVVMHLSPTLWGVMVAMT
                     VIGFCGGIGLCIAGTIVVSVWQDRIQSTMLVVQDATFNVAGVVFPLVTTYALTHNMSW
                     SYSYLTVGVVALLTLAVVILTRFNLCELSAPSDSSPIVKSEWNSGIISGGIGLFLGML
                     ALYTFLTWSPLFVKNKFGIPFEEAGNIITQYWAAALIGALVSTVIVTRVKIHYFLLCI
                     ISLACIITTIIVTKESLSGLDYLAYSYGFVCAALYNSFIAYGVSFVRRASSQNVSYIL
                     ISGSAGAMFSPAISSLMEKMMGLQKIMYAIPILYLVIFIMLVTTLKIKNEVAK"
     gene            complement(2902..3609)
                     /gene="deoD"
                     /locus_tag="OVW45_004298"
     CDS             complement(2902..3609)
                     /gene="deoD"
                     /locus_tag="OVW45_004298"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCV5528588.1"
                     /translation="MTAHINAKPGDFAPTVIMPGDPLRAKFIAENYLQDHREVTNVRN
                     MLGYTGFYSGQLISVMGHGMGIPSMTLYAHELVNDFDVKRIIRVGSLGATQRTIKMRD
                     IILAVASGTDSVTNFKRSSGYFMASSAYFPLLQKAWSLAQEAGLEVKAGNVFSGDIYY
                     DPDDNLIPALEKFGVLGIDMEVAGLYALAHQFGIEALAILTVSDHCLTGEETTAEERQ
                     LTFNNMIELALNTAVAI"
     gene            3901..4243
                     /locus_tag="OVW45_004299"
                     /pseudo
     CDS             3901..4243
                     /locus_tag="OVW45_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     misc_feature    4182..>4243
                     /locus_tag="OVW45_004299"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT         1269 a          956 c          884 g         1134 t
ORIGIN      
        1 gcatagagtt aatcatccag aatttttctc gaatgaagct tttcatgcag cttaaaccac
       61 tcacaaaagg tcgttttcat aatatctggc agcaaaatga tgctttcatt gagaaaacgt
      121 ttcgtgaact gctttctgaa acccagctaa tccatgaagg cgactatctt tttcgccagg
      181 gacaggtggt cgaccgtctg atcatggtgg agactggccg aatatccttg agctacagtg
      241 ctgaaaatgg tcgctgtttc cagttcggaa caatggattg cgacgaacag ttgtttggtg
      301 aaatggagtt ctttacaggt tatctctgtc aacttgatat tgtcgcagaa gaacctctca
      361 tcgttggggt atgtagtagt gaacgccttg aggctgcgct ggatcaacat cctaagctgg
      421 ccttgttctt tgccagcgct atagctgtcg actaccagga tacactggaa atcttcttac
      481 atcggatgct ctatcctatt gcgcacaaca tcgcctatga tctgtatcgc cagcatcaaa
      541 ataagcaacc gatggacggt tttagtaaaa attataagga agctgaacga tttggtacca
      601 cagatcgcgt ttatcgccgt gcagtaaaaa ctctggtaga tgccggtctg gttattcgta
      661 ccaaacaggg gctggaaatt aagaacttag atgagttaag gcgattcttg ggtaacgact
      721 cgtttgatta aacgccagta ttatcgctga taatactggc gtgttctgag aaaagaggct
      781 gtgcttagaa cttgtaggtc aaattgaaat aatgccctaa acctgttgtt cgacctgctc
      841 caccgtggtt ttgcatcaac gccatgttac ggaagtattt cagtccatag ccaatagcat
      901 agttttcaga gtgccagtaa ataccgttgt accattcaat agcgttgttg ctgtagagcg
      961 cctggtcggc aatcttgttg gctgcaaact gatagtcaaa atatccctga tagttcaggt
     1021 aactaccgtt cgcaaagttc cagacaggcg tagaccagtt agtggaaaac atataaccat
     1081 cccactttcc ttcgttatct gcgccatagt tttcgcggac ataatgggca tagacgttca
     1141 tccctaccag acccaaaaac ggtaccttca catcggtgcc catgccaaca tagtgctcaa
     1201 aaagctcacg atcgccaata ttgttaaccg tagcaatata aaattcgtta aacggaccga
     1261 cagaaaggtc cttattaaaa atggcatcca gagaaaaacg tggtgaaatc ttggcaaaga
     1321 agttatcgcc accgtggcgg tcatcatgct tgttgtcgaa aacatcgaag aaatccacat
     1381 aaccatagag atcgactatg ccagatcgag caccaaattc cagttccagg taggtatcat
     1441 cgcgaatacc atatggaact tttgcatcaa cactctgcat taaattgatt tgcatccagt
     1501 tgaagtcatt tttccgaata tttccatctg cgtaatcagc agcaaatgta tttactgatg
     1561 ccatgcctat cgccatggat attaaaacta tcttaacgtt cattttattt gtatgtgaca
     1621 tattccattc ctttgcacaa gtttgagtta cgcgttcccc gaaggacaaa gaaatatctc
     1681 atcttagagt gaggtaaaac cttattttgc tacttcattt ttgattttca atgtggtaac
     1741 caacatgatg aaaataacaa gatacagaat cggtattgca tacattattt tctgtaaccc
     1801 catcatcttc tccatcagag aactaatagc agggctaaac attgcaccgg cactgccgct
     1861 tatcaaaata taagaaacat tctgactact ggcgcgcctg acaaaagaca ctccgtaggc
     1921 aataaacgaa ttatataaag cagcacatac gaacccataa ctgtaagcca gataatcaag
     1981 cccagagagg ctttctttag ttacgataat ggtagtgatt atgcaggcta atgaaataat
     2041 acacagcagg aaataatgta ttttcactcg ggtaacgata accgttgaaa ccagagcacc
     2101 aatcagtgca gcagcccaat attgggtaat gatattcccc gcctcttcaa atgggatacc
     2161 aaatttattt ttaacaaata aaggcgacca cgtcagaaaa gtatagagcg ccagcattcc
     2221 cagaaataat cctatgcctc cactaataat acctgaattc cattcagatt tgacaatggg
     2281 ggacgagtca gagggggctg acaattcgca aagattaaaa cgtgtgagta tgacaactgc
     2341 taacgtaagc aatgcaacta cgccgacagt taagtagcta tagctccagg acatattgtg
     2401 cgtaagcgca taggttgtta ccagtgggaa aacaacacca gccacgttaa aggttgcgtc
     2461 ctgcactacc aacatcgtgc tttgtatacg atcctgccag actgaaacca caatagttcc
     2521 tgctatgcac aaaccaatcc ctccgcagaa accaatcacc gtcatcgcta ccataactcc
     2581 ccataatgtt ggactcaggt gcatcacgac agcacagagg aaaacgagag aataaatgat
     2641 gaacgtcatg cgcttcacac cgattttttc tataaggaaa aaggcggcga ttgtgcctgc
     2701 cagagcacca ccattaagta aggaaaatat tgaagctaca gcattgatat cagcatcata
     2761 ccgctgggca atgggttcaa tcagcatgcc aaactgactg gcaaatcctg ccataatgaa
     2821 atttgccaag aaactaatca gcgtaagtgc aattttattt ttcatataat gacagccctg
     2881 attatgttat gagatgaaaa tctaaatggc gacagcagta ttcagcgcca attcaatcat
     2941 gttgttaaat gttaactgtc gctcttctgc tgttgtttct tctcctgtca gacagtgatc
     3001 ggataccgtt aaaattgcca acgcttcaat accgaattga tgtgcaagtg catataaccc
     3061 tgcgacttcc atatcgatac ctagcactcc gaatttctca agtgccggaa ttaaattatc
     3121 atccggatcg taataaatgt cgccgctaaa tacattgcca gcttttacct ctaaaccggc
     3181 ttcctgagct aatgaccatg ctttttgtaa taaggggaaa tatgcggagg acgccataaa
     3241 gtaacccgaa ctacgtttaa aattagtgac agagtctgtt ccggaagcga ccgctaaaat
     3301 aatatcccgc attttaattg tacgttgtgt tgcaccaaga ctgccaacac gaataatccg
     3361 ttttacatca aaatcattaa ccagttcatg ggcatataat gtcattgatg gaattcccat
     3421 tccgtgcccc atcacagaaa tgagttgccc tgaatagaat cccgtgtatc ccaacatatt
     3481 ccgaacattt gtgacttcac ggtggtcctg cagatagttt tcggctataa atttcgccct
     3541 tagaggatct cctggcataa ttactgttgg tgcaaaatcg cctggtttag catttatatg
     3601 tgcagtcatt tttattcctg tatgtcttga gtttaagaaa cccaaggatt gtaaccagac
     3661 acagaaagag aaatgaggac aaaagtcctg ctcaaaagat cgaactctca tttttgaatg
     3721 ttaatattta tactcctgct tgagcaaaac gaaggtcgcc gtcttcctgg cattctcgcg
     3781 gatcatcaaa gatgaccgat agcgattttg tcattatggg cgcaacacca acgagtcaaa
     3841 cgcttttgaa ccgccccggg aatcctggag actaaacttc ctgagaaaga ggtaaacagg
     3901 atgactaaaa atactcgttt ttcccccgaa gtccgtcaac gggcagtccg tatggttctg
     3961 gaaagtcagg gcgaatatga ctcacaatgg gcgacaattt gttccattgc tccaaagatt
     4021 ggctgtacgc cggagactct gcgtgtctgg gttcgccagc atgagcggga taccgggggc
     4081 ggtgatggcg ggctcaccac cgctgaacgt cagcgtctga aagagctgga acgtgaaaat
     4141 cgtgaactgc gccgcagtaa cgatatcctt cgccaggctt ccgcttattt tgcgaaggcg
     4201 gagttcgacc gcctctggaa aaaataatgc cactgctgga taa
//
LOCUS       DAKISA010000211         4192 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.214, whole genome shotgun sequence.
ACCESSION   DAKISA010000211 DAKISA010000000
VERSION     DAKISA010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.214"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..215)
                     /gene="tuf"
                     /locus_tag="OVW45_004300"
     CDS             complement(<1..215)
                     /gene="tuf"
                     /locus_tag="OVW45_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCV5528589.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(330..405)
                     /locus_tag="OVW45_004301"
     tRNA            complement(330..405)
                     /locus_tag="OVW45_004301"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(370..372),aa:Thr,seq:ggt)
     gene            complement(412..486)
                     /locus_tag="OVW45_004302"
     tRNA            complement(412..486)
                     /locus_tag="OVW45_004302"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(451..453),aa:Gly,seq:tcc)
     gene            complement(603..687)
                     /locus_tag="OVW45_004303"
     tRNA            complement(603..687)
                     /locus_tag="OVW45_004303"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(651..653),aa:Tyr,seq:gta)
     gene            complement(696..771)
                     /locus_tag="OVW45_004304"
     tRNA            complement(696..771)
                     /locus_tag="OVW45_004304"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Thr,seq:tgt)
     gene            1133..2083
                     /gene="coaA"
                     /locus_tag="OVW45_004305"
                     /gene_synonym="panK"
     CDS             1133..2083
                     /gene="coaA"
                     /locus_tag="OVW45_004305"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCV5528590.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2112..3077)
                     /gene="birA"
                     /locus_tag="OVW45_004306"
     CDS             complement(2112..3077)
                     /gene="birA"
                     /locus_tag="OVW45_004306"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCV5528591.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3074..4102)
                     /gene="murB"
                     /locus_tag="OVW45_004307"
     CDS             complement(3074..4102)
                     /gene="murB"
                     /locus_tag="OVW45_004307"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCV5528592.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1081 a         1042 c          935 g         1134 t
ORIGIN      
        1 gtgtcgtatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc gcgagcagca ccgccgtagg ttttagccag tacggtagtg
      121 attgcagcgg tcagcgttgt tttaccatgg tcaacgtggc cgatagtacc gacgttaacg
      181 tgcggttttg tacgttcaaa cttttcttta gacatcgatt gtccctctaa gacacggata
      241 aatcggtgat atcaccacat caaccaggca acatgcccga cttgttgaat gcaataaaca
      301 gaaggaaaaa acagggagga gaaaagaagt ggtgctgata ggcagattcg aactgccgac
      361 ctcaccctta ccaagggtgc gctctaccaa ctgagctata tcagcacatc ttggagcggg
      421 cagcgggaat cgaacccgca tcatcagctt ggaaggctga ggtaatagcc attatacgat
      481 gcccgcatcc tggaactcgg ctacctgatt ttcattctgc actgaatatc gagagaagct
      541 ctctttattc gagccggtaa gcgaacttat cgtctcgggc tacgccatcg cgtggccgaa
      601 attggtggtg ggggaaggat tcgaaccttc gaagtctgtg acggcagatt tacagtctgc
      661 tccctttggc cgctcgggaa ccccaccgga cttgatggtg ccgactaccg gaatcgaact
      721 ggtgacctac tgattacaag tcagttgctc tacctactga gctaagtcgg catcaagtag
      781 cgcgcattct atggagacat gcgagttcat gcaactaaaa aattgcataa tttgttttat
      841 tggtcacatt ttatgcgaca cgatgaagaa acagccgaac ggagggcaga ttagcacact
      901 ttttcaacat cattgtgctc aacaatgcgc tcctgctaaa ccataattct ttttatcaga
      961 tggaatatct gtcacattgc ttttcaacga tagcttcctg gcagagattt tttcttatta
     1021 ttcctcccca tctggtgtta ccctcctgcc cattaaccca ttcaacagaa ctgtgacgcg
     1081 ccatggcaaa tatcgctttg ccgatagagc tatgaccgcc agaaacatgc ttatgagtat
     1141 aaaagagcaa acgttaatga cgccttacct acagtttgac cgcaaccagt gggcagctct
     1201 gcgtgattcc gtacctatga cgttatcgga agatgagatc gcccgtctca aaggtattaa
     1261 tgaagatctc tcgttagaag aagttgccga gatctattta cctttgtcac gtttgctgaa
     1321 cttctatata agctcgaatc tgcgccgtca ggcagttctg gaacagtttc ttggtaccaa
     1381 cgggcaacgc attccttaca ttatcagtat tgctggcagt gtcgcggtgg ggaaaagtac
     1441 aaccgcccgt gtattgcagg cgctattaag ccgttggccg gaacatcgtc gtgttgaact
     1501 gatcactaca gatggcttcc ttcaccctaa tcaggttctg aaagaacgtg gtctgatgaa
     1561 gaagaaaggc ttcccggaat cgtatgatat gcatcgcctg gtgaagtttg tttccgatct
     1621 caagtccggc gtgccaaacg ttacagcccc tgtttactcg catcttattt atgatgtgat
     1681 cccggatgga gataaaactg ttgttcagcc tgatatttta attcttgaag ggttaaatgt
     1741 cttacagagc gggatggatt atccacacga tccacatcat gtatttgttt ctgattttgt
     1801 cgatttttcg atatatgttg atgcaccgga agacttactt cagacgtggt atatcaaccg
     1861 ttttctgaaa ttccgcgaag gggcttttac cgacccggat tcctattttc ataactacgc
     1921 aaaattaact aaagaagaag cgattaagac tgccatgaca ttgtggaaag agatcaactg
     1981 gctgaactta aagcaaaata ttctacctac tcgtgagcgc gccagtttaa tcctgacgaa
     2041 aagtgctaat catgcggtag aagaggtcag actacgcaaa taatttgcag gggagcgaat
     2101 actccccttt cttatttttc tgcactacgc agggatattt caccgcccat ccagggtttt
     2161 attattccat cctgctcaag taataaagcc ccctgtttgt ctattccgcg tgaaatgcca
     2221 aatatttctt tatcaccaat gataagtttc actgggcgat taataaaatt atccagcttt
     2281 tcccagcgcg acagataagg tgccaatcct tcttgttcga agagttccaa cgcagcacgt
     2341 aattcacgta ttagcatggc cgccaacgta ttacgatcga gattgatccc cgcttcctgc
     2401 agcgtgatcc acccctgatt aacgacactc tcttcaacac ggcgcattgc catgttgatc
     2461 ccggctccaa tgactatttg cgccgcatcg ccagttttgc cagtcagctc cacaagaatg
     2521 cctgccagct tgcgatcctg cagatagagg tcattaggcc atttgacacg aactttatct
     2581 gctcccagct tgcgtaatac ttccgccatc acgataccga taaccagact taaaccaatc
     2641 gccgccgccg ggccttgttc cagacgccag aacatcgaca aatataagtt tgcgccaaaa
     2701 ggcgaaaacc atttccgccc ccggcgacca cggccagcct gctggtattc tgcaacacag
     2761 gcatcgcccg atttaagctc tccgatacga tcaagaaggt actgattcgt ggagtcaata
     2821 actggcagca cggctacact accgccatcc agctgaccca atatctgttc agcattaagt
     2881 aactggatgg gctcaggcag gctgtatcct ttacccggaa cggtaaagac atcaacgccc
     2941 cagtcacgca gtgtctgaat gtgtttatta atagccgccc ggctcattcc cagcgtttca
     3001 cccaactgct cgccagagtg aaattcaccg ttcgctaaca gggcaatcaa tttcagtggc
     3061 acggtgttat ccttcatgaa attgtctcca ctgcgctcac ttcacctgat gcaccaataa
     3121 agcggacttc aggctcaagc cagacattaa atttttcacc cactttttgt cttacatggt
     3181 gtgccagttg caccacatct tcgctttttg cattgtcttc attaatgaga actaacgcct
     3241 gttgacggtg caccgcagcc ccacccattt gcatcccttt tagctggcac tgatcaataa
     3301 gccaacctgc tgccagtttt actgaaccac ccgcctgggg gtaatttggt gctgttggaa
     3361 attgcgccag taatgcttta gccgtttcgg cagatacaac agggtttttg aagaaactac
     3421 cggcattgcc attcactttt ggatcaggga gtttggtggt gcgcatatga cacaccgcat
     3481 taaatacttg ctgtggcgtt actgttgtag gatccagacg agttaagtca ccatacgtta
     3541 gtacaggttg ccactctttt ggcagacgca gacctacggc tacaatggcg aagcgatcct
     3601 ggtattcatg tttaaaaata ctgtcgcgat agccaaaacg gcactctttg gcagttaagc
     3661 gcacttgctt gcctgtcgcc agttcaacac aatcaacata agcgcaaact cgctgtaatt
     3721 ctacgccata agcaccaata ttctggatag gtgatgagcc gacacaacca ggaattaatg
     3781 ccagattttc cagaccaggc ataccttcct gcaaagtgta ttttaccagg cgatgccagt
     3841 tttctccggc tcctacatgt aaataccacg catcaggttc atcatgaatt tcgatacctt
     3901 tgatccggtt gatgatcacc gtgccacgat agtcttccag aaaaagtaca ttacttcctt
     3961 cacccagaat aagaacgggt tgtccttctg cggttgcatg ctgccaggca ttgagtaatt
     4021 gttgttcgtc ttcggcacat acaatgtgct gagcattatg atcaatgcca aatgtgttcc
     4081 agggttttaa ggagtggttc atagctgctt tcctgatgca aaaacaaggc tagtttaccg
     4141 tatctgtggg gggatggctt gtagatatga cgacaggaag agtttgtaga aa
//
LOCUS       DAKISA010000212         4187 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.215, whole genome shotgun sequence.
ACCESSION   DAKISA010000212 DAKISA010000000
VERSION     DAKISA010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.215"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(184..375)
                     /locus_tag="OVW45_004308"
     CDS             complement(184..375)
                     /locus_tag="OVW45_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528593.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGQYILQGSRNR"
     gene            complement(375..794)
                     /locus_tag="OVW45_004309"
     CDS             complement(375..794)
                     /locus_tag="OVW45_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HCV5528594.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFVYGACDRVVSDIVNELMEEKRVNRLVTVPAVLLEKVMVMAGSEI
                     YRLHAVGSENGGDGDAFVREEREIMRVMRQALDGENG"
     gene            complement(841..1266)
                     /locus_tag="OVW45_004310"
     CDS             complement(841..1266)
                     /locus_tag="OVW45_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCV5528595.1"
                     /translation="MQYAKSVTLNVEECDRLSFLPYLFGQDFLYAEASVYALAKKMMP
                     EYEGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGVILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            1836..2558
                     /locus_tag="OVW45_004311"
                     /pseudo
     CDS             1836..2558
                     /locus_tag="OVW45_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            2632..2889
                     /locus_tag="OVW45_004312"
                     /pseudo
     CDS             2632..2889
                     /locus_tag="OVW45_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2902..4053)
                     /locus_tag="OVW45_004313"
     CDS             complement(2902..4053)
                     /locus_tag="OVW45_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
                     /protein_id="HCV5528596.1"
                     /translation="MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDT
                     GGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIRAIATELNRSPSTISREVQRNRGRR
                     YYKAVDANNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQK
                     TLRISPETIYKTLYFRSREALHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVNGTPI
                     HERSRNIDNRRSLGHWEGDLVSGTKNSHIATLVDRKSRYTIILRLRGKDSVSVNQALT
                     DKFLSLPSELRKSLTWDRGMELARHLEFTVSTGVKVYFCDPQSPWQRGTNENTNGLIR
                     QYFPKKICLAQYTQHELDLVAAQLNNRPRKTLKFKTPKEIIERGVALTD"
BASE COUNT         1060 a         1104 c         1008 g         1015 t
ORIGIN      
        1 agacggttac acaagcacgg taaccacaga aaaataacat cagaaaaaaa acattccgga
       61 cacatcacaa acaggaaggg taacgggcga cgcgtcagcg tggcccgctt cccttccggc
      121 aggcgtcagc ctgccagggg ttttcgcgcg ggctgacagg cggcctccac cgcctgccct
      181 gtatcacctg tttctgctgc cctgaaggat gtactgaccg ccgaggaata cagcctgctg
      241 attcgccaca aaatcgcaga tttcatcaac aatctgcgcc gtggtgagcg gtttctgccc
      301 catttcacga cgtcgtgcat taatcacagc tttgtaatta cgcagaattt cgcgggtttc
      361 cgtgctgata ttcattaccc gttctccccg tccagcgcct gacgcatcac ccgcataatc
      421 tcacgttctt ccctgacaaa tgcgtcaccg tcgcccccgt tctcgctccc gacagcatgg
      481 aggcgataaa tttcactacc agccatcacc atcacttttt ccagcagcac cgcagggact
      541 gttacaagac ggtttacgcg cttttcctcc atcagttcat tgacaatatc ggaaacaact
      601 ctgtcacatg cgccatatac aaaagcatcg tccagccgct gcatgacggt ttccagctca
      661 tcgtctgtca gactccagtc ctgggctatg tgacgcacat ccccacgggt cagcaccacg
      721 gcaaaaacgc attcactgtc cggcacgtct gtatccagta catcgcggat gatctcttta
      781 acagtacagt acatgttctc gtctccttta caggggcggc attgccgccc cgtccgttaa
      841 tcagtccagt gccgcgtaaa tcgcgttgca ttcagggtga aattcgatgt gacgccacaa
      901 ctgcgcatca cgcatccggt aaagctgagt cagccccgca tcaccgctgt cgtggtacag
      961 ccacaactga cggttaatca caagggaagt cagaatgaca cctgcggcat cagcactcac
     1021 ggtacggtca aaccagtttg caccgttcac catgtggaaa cggtcgccat ccggcatcat
     1081 gtaaccgcca ccgtccggca ggcggatgaa gtgccagaat ccgccttcat attccggcat
     1141 catttttttc gccagcgcgt atacagacgc ttcggcatac agaaaatcct ggccaaacag
     1201 gtaaggcaga aaagacagac ggtcgcactc ttcaacgttc agagtgacag atttcgcata
     1261 ttgcatgatg ttttcctccg ttgttcacat attccgcgac accgggcggt gccgtgcctc
     1321 cataaaagcc gggcgataag gcggggatgc aggggcgcaa gggaagccgc agcctgacgc
     1381 acacggctga cgggagttgg agggtgagac cgcgaaatct gagcgggtga acgaccggaa
     1441 aatcacggca gacgggaaga agggaccggg tgaccgcagc ggcaaagcgt acccttgcat
     1501 ccccgccagc ccggctaaga tggaggcacg gcacagcccg cacacgacac ggcggccaca
     1561 gagctgaaaa cccacaaggc catgccccgc atggccgggt aaagcggagc cggcggcgac
     1621 ttccttgtat aattatccca cacagaacag attggttctg tagttaacac tggggcaaag
     1681 caggccggcg acttcctttg gccagtgagc cagtcaaaaa gataattgcc gctttgcctc
     1741 tcttttcagt gtatgaccga agggtatctg tggccccgta catcggtgag cctgaatttg
     1801 caaggtaggt cagctgaact aaccgaggaa aagctatgac tgtgtacatt ggaattgata
     1861 ttgccagaaa gaagtttgat gcagctgaac tctgtgacgg ggcttacagg tgtaagacat
     1921 ttgaaaacac ccctgctgga tttacagcgc tatacgactg gcttaaagcg ggcagcattc
     1981 cggacattca tgtatgtctt gaagcaacag gcaactatgg tactgccctc gctacttttc
     2041 tgtacgaaaa gaatctgaaa gtgagcgtag ttaatcccgc aagaatcaag ggattcgcgg
     2101 cttctgaact tacccgaacc aaaaccgaca aagctgactc cagactgatt gcacgctttt
     2161 gtaaagccgc gcagccagat ccatggagac ctgtgcccgc ccccgtcaga aaacttcagg
     2221 ctctagtgag acgtatcggt gcgctgaacg gcatgctcag aatggaatgc aaccgtcgtg
     2281 aaaatgcaga tattgacgct caggaatcgg ttgaacgcat gatcaaccat ttaaagatgg
     2341 agcttgaatc ggtgcgccgg gcaataaaag agcatatcag taatgttcct gagctgaaaa
     2401 aacagaacga cctcctgctg tctattccgg gtattggcac tgtttcagcc agcctgatac
     2461 tgtccttcat ggccagtaaa gagttcacaa aagctaagga agtgaccgct tttctggggc
     2521 tcaatccccg ccatcaccag tccggaacgt cggttagggt gaagtggcac actgaatttg
     2581 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
     2641 agaaatttca gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaactac
     2701 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
     2761 aaacagctgc gtgatgagcg tcagggcaaa acaccaaaag cctctccgat aacaccagaa
     2821 caaatcgaaa tacgtgagct gaggaaaaag ctacaacgca ttgaaatgga gaatgaaata
     2881 ttaaaaaagg ctactgtaga ttcaatctgt caatgcaaca cccctttcaa ttatctcttt
     2941 cggtgttttg aacttcagtg tctttctcgg tctgttgttt agctgagcag caaccagatc
     3001 tagttcatgt tgagtatatt gggcaagaca tatcttttta ggaaagtact gccgaattag
     3061 cccatttgtg ttctcatttg ttccccgctg ccaaggactc tgaggatcgc agaagtaaac
     3121 tttaacgccg gtgctgacag taaattctag atgtctggcc agttccattc ctctgtccca
     3181 tgtcagtgat tttctgagtt ctgacggtaa actcaggaat ttgtcggtaa gagcctgatt
     3241 tactgagaca gaatctttgc ccctgagtct aaggatgatc gtataacgtg attttcggtc
     3301 tacaagtgtg gctatatgag agttttttgt acctgagact aaatcgccct cccaatgccc
     3361 cagagagcgt ctgttatcga tatttcggga acgttcgtga attggtgttc cgttcactat
     3421 gttaatcgta cctctttcgc ctttgcgggt atgacgcctg ccatggcgaa ggctatgcga
     3481 ccgtcgcaga tgctgtatat tcaggtggtg tagcgcttca cggctacgaa agtacagcgt
     3541 tttataaatt gtctcaggtg atattcgcag cgttttttga cgtggttttg ttcgccttaa
     3601 ccatcctgat atttgctctg gagaccattt catctccagc ttttccagaa caagctttcg
     3661 caatggtaaa ttttgatcca gtaagcacgg ttttggcctt ttcgccattc tgttggctcg
     3721 gttattagca tcaacagctt tgtaatagcg tctgccccga ttacgctgaa cttcacgtga
     3781 gatcgtcgaa ggactgcgat tcagctcagt agctatcgca cgaatgctca ttttggctga
     3841 caaaccagct cgtatctcct cgcgctcaga cagtgtcagg tgagctacag cccgcttacg
     3901 ctcatggggt tttatgccgc cagtatccct taacatagtg aagatcgttc cgggttttga
     3961 acccaggata ttcgctattt cactgaagcc tgttccgttc ttccatagtt caaaaacaga
     4021 ggctttttcc tctgctgtaa atgttcgtct cattcaaaaa acctccgcaa ccccatgttt
     4081 tcacataact gttgcgttga ccaattgaat ctacaaccgc gctcttgatg tcagactccc
     4141 tgaacagttc tcgataatcg ggaaactcag agcgcattat cctgtgg
//
LOCUS       DAKISA010000213         4125 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.216, whole genome shotgun sequence.
ACCESSION   DAKISA010000213 DAKISA010000000
VERSION     DAKISA010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.216"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..335)
                     /locus_tag="OVW45_004314"
     CDS             complement(<1..335)
                     /locus_tag="OVW45_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCV5528597.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCMELVASGDVYNSGYGVFPSEQAHKDWQNARKKLSRVKVKKPVVVDPDLIWSLPDG
                     EIRRYDRRL"
     gene            complement(350..1096)
                     /locus_tag="OVW45_004315"
     CDS             complement(350..1096)
                     /locus_tag="OVW45_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCV5528598.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLADAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            complement(1103..2302)
                     /locus_tag="OVW45_004316"
     CDS             complement(1103..2302)
                     /locus_tag="OVW45_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HCV5528599.1"
                     /translation="MLFVLILSNRAASYGAIMAALPYMQLYIADYLADTMHLSAEEHG
                     AYLLLMFNYWQTGKPIPKNRLAKIARLTNERWVDVEPSLREFFCDNGDEWMHLRIEED
                     LASVREKLTKKSAAGKASVQARRSRKEADVQTKQERNLTGVQTDVGVMFEHDANTKAT
                     NKDTDKDLKTDLTHPKPFPSGREFRDFVAGVLEGRLSGGTAAEFCNSAVVALQAAGLD
                     VCREYPVPERGDGCGGRIDIVVTDRNGVRCGSELDRNSPRQKSLLKIGAVETGICVLR
                     RSDIARHTEQGILVIGGAVRQKKFDPLSVDLPDWLPETLWHEWVQFRQALRKPIRTEQ
                     GANGAIRELEKFRQQGFTPEQVIRHSIANEYQGLFAPKGVRPETLLRQVNTVSLPDSA
                     IPPGFRG"
     gene            complement(2315..2737)
                     /locus_tag="OVW45_004317"
     CDS             complement(2315..2737)
                     /locus_tag="OVW45_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528600.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSSDDSVIVH"
     gene            complement(2734..2976)
                     /locus_tag="OVW45_004318"
     CDS             complement(2734..2976)
                     /locus_tag="OVW45_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCV5528601.1"
                     /translation="MNKISTYRKQLGLSQRQLAVQLGWIQSRLANYEANFRTPGLEEC
                     RKIVSTLNRLGAHCGLDDVFPPDGKHSENSIGAVDS"
     gene            3073..3492
                     /locus_tag="OVW45_004319"
     CDS             3073..3492
                     /locus_tag="OVW45_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCV5528602.1"
                     /translation="MEKENEKIAASRLNDKIAMRLKERRQKLGLSQGKLAEICGWTQS
                     RIGNYEAGSRNVGVHDAVVLGKALGISPPELLFGEQESSELWLNESQRKLLELFNQLP
                     GSEQQRMIELFEVRLKEIDEYVEKYLRGRIKDNPPPE"
     gene            3798..3953
                     /locus_tag="OVW45_004320"
     CDS             3798..3953
                     /locus_tag="OVW45_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCV5528603.1"
                     /translation="MNTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            3955..>4125
                     /locus_tag="OVW45_004321"
     CDS             3955..>4125
                     /locus_tag="OVW45_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528604.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGR"
BASE COUNT         1041 a         1103 c          927 g         1054 t
ORIGIN      
        1 ttcaggcgcc tgtcgtagcg gcgtatttct ccgtctggta atgaccagat aaggtcagga
       61 tcaaccacaa ccggtttctt cacctttacc ctcgatagct ttttgcgggc gttttgccag
      121 tctttatgag cctgttcaga gggaaatacc ccgtagccag aattatatac atcaccactt
      181 gcgaccagtt ccatgcataa gcgaccgaca gacgcacgac tgatacctgt ttcatctgac
      241 aactgccgaa tcgtgacccg accgtcctga tgcacgagtt ccacaattcg cgtcttcagt
      301 tcttcccgct gttcgggagt aaaaggtttc gccataaatc ctcctgaaac tacttaacaa
      361 ccctcgaatg gctaacattc ggacgccagc tctcccagtt aaaattcacc cagcgcccgc
      421 cgttcatgac catgcgatcc atcacccgct cgccgagaag tgtattcatc gctgcatggt
      481 taatatttgt cagcatcccc acaccgcgta acgatgccgt ccggcgatca acaatctggt
      541 tcagtaccac ctgctcgttt ttcgtatccc gctgcatgcc aatttcatcc aggaccagca
      601 ggtcaacacc acaaagctcc tgtaaaaatt tttcaccgga tttgccgttg tcgtagctct
      661 catgcaacac gctcatgaca tcggacacgg tgacgataat cacgctacgc cccttcgcca
      721 tcagccggtt gccaatcgcg tctgcaagat gattttttcc ggtgccgggc ttaccgctga
      781 acacaaaatt cgtacagcct gtcatcagtt cgtcagctat ggatttcgcc tgactcagtg
      841 catgtcgctg accgtcgttc tgcgcccggt aattcgcaaa cgaacacttc cggtgcaacg
      901 gctggatgcc ggagcggttc aggatttttt ccacccgcaa ctgacgattc aggcggttga
      961 tctcctcgca acgtttctgg ccttcagcaa gttgccactc gcgccactcc gcaaccgttc
     1021 tgaatggggc ggttacatgt ggtggggtca gtctgcggat acgctccaga acgcctcctg
     1081 tcgcaatatt tttcatggtc tgttaccccc tgaagcctgg cgggatcgca ctgtccggca
     1141 acgagacggt gttaacctgt cggagcaacg tctcaggccg aacacctttc ggcgcgaaca
     1201 gaccctggta ttcattggcg atgctgtgtc gaatcacctg ctcaggtgta aaaccctgct
     1261 gacggaattt ttccagttcc cgtatcgccc cgttagcgcc ctgctccgtt cgaatcggtt
     1321 ttcgcaatgc ctgcctgaac tggacccact catgccagag tgtttctggc aaccagtcgg
     1381 gcagatcaac tgacaacggg tcaaattttt tctggcgaac agccccaccg ataaccagaa
     1441 ttccctgctc ggtgtgcctt gcgatatcac tgcgccgcaa gacacatatc ccggtttcaa
     1501 cagcaccgat tttgagcagt gatttctgtc gcggagaatt tcggtcaagc tcgctcccac
     1561 accggacacc gttcctgtca gtcacgacga tatcaatccg ccctccgcaa ccgtcaccac
     1621 gctctggcac cggatactca cgacagacat ccaggccagc agcctgcaac gcaaccaccg
     1681 cagaattaca aaattccgct gcagtaccgc cagataatct cccctcaagc actccagcca
     1741 caaaatcccg aaactccctt ccggaaggga agggtttggg atgggttaga tctgttttta
     1801 gatctttatc tgtatcttta ttagttgcct ttgtgttggc atcatgttcg aacatcactc
     1861 caacatctgt ttgaacacct gttaaatttc tctcttgttt tgtttgaaca tctgcttcct
     1921 ttctgcttct tctggcctga acagatgctt ttcctgcggc tgattttttg gttaattttt
     1981 ctctgacaga tgccagatct tcctcaatcc gaagatgcat ccattcgtcg ccgttatcac
     2041 aaaaaaactc ccgcaaggat ggttcaacat caacccatcg ctcgttagtc agacgggcaa
     2101 tttttgccaa cctgtttttg ggtattggct ttcctgtttg ccagtaattg aacatcagca
     2161 acaaatacgc gccgtgctcc tctgctgaca aatgcatggt gtcagccagg taatcagcta
     2221 tgtacagttg catgtatggt aatgcggcca taattgcccc gtatgatgct gcccggttgc
     2281 ttagaataag cacaaacagc atggaaactt ttgcttaatg aacaatgaca gaatcgtcgg
     2341 aagacccgcc gccgctgaaa tgcgctttcc ggtaaacggc ctggactgca tcatcatgcg
     2401 catcaattgc cgtactcaac gcttcctgcg ccgccagtaa tgcacggcgt tccagggtat
     2461 cgaagatgca gagtcggtga cgcagctcgc gcggaagaat tgccagaacc gcagggatca
     2521 gtttctgaat tttttccctt tgcgctttcg tttcaccttt caaccaacgg tgatagatat
     2581 tctgctgatt gttccagtcc ttgcctggta caaggggcaa ttcgccgccc ccctggcgca
     2641 gatattcttc agtaattgcg ttagcgaccc acgcctgccc tttttcggct gccagggcta
     2701 acaacactga ttcgatgtgc tcatgcctga ttttcatgaa tcaaccgctc ctatgctgtt
     2761 ttcgctatgc ttaccgtctg gggggaatac atcgtcaagt ccacaatgag cgccaagccg
     2821 attaagggta gaaacaattt ttctgcactc ctctagtcca ggggtacgaa aatttgcttc
     2881 gtaatttgcc agtcggcttt gtatccaccc taactgaaca gcaagttgtc tttgagacag
     2941 cccaagctgt tttcgatatg ttgaaatttt gttcattgaa aacctccgat gacaatttta
     3001 aacacacctt gtgttatata gccaagctgt tttgtgtttt atgtaaatca cgattcgtga
     3061 tacaaggatg caatggaaaa agaaaacgaa aaaattgccg ctagtaggct caatgacaaa
     3121 attgcaatgc gtcttaaaga gcgcaggcag aagcttggtt tatctcaagg aaaacttgct
     3181 gaaatctgcg gatggacgca atcgcgtata ggtaactatg aggcgggcag cagaaatgtt
     3241 ggagtacatg acgctgtcgt attgggaaag gcacttggca tatctcctcc tgagctcctc
     3301 tttggagaac aggaatcttc tgaattgtgg ttaaatgaat cccaacgaaa acttcttgag
     3361 ttgtttaacc agctaccggg ctcagaacaa caacgaatga ttgagctatt tgaagtccgg
     3421 ctaaaagaaa tcgatgagta tgtagaaaaa tatttgagag gcaggattaa agataatccc
     3481 ccaccggaat aatgatcttg ctatcacagt aatatgccaa tcagcccgct atcagcgggc
     3541 ttttttgtag catcatcata tgacactcac cataaaacac gttgcgtgtt gacacaagaa
     3601 aacaaattgt gtttaataag catatccaaa caacgcccca ccagagaacg gcaggacaat
     3661 acctcgagtt atccagtcac tgaacagggc taagtagcca gcctgaggca tacgaacatg
     3721 acggcagttg ttgattgata caaagcgcag tagataaaac gttccgccac ccggcgttaa
     3781 ggggaaatgg ggtcaacatg aatactatcg atcttggcaa caacgaatct ctggtatgtg
     3841 gcgtgtttcc caatcaagac ggcacattca ccgccatgac gtataccaaa agcaaaacgt
     3901 ttaaaaccga agctggcgca cgtcgttggt tgggaagaca ttcaggtgag taaaatgaac
     3961 gagacagaat taaaacacgt tatcgctcta ctcctagaag atgcaaaacg cctccagcaa
     4021 ctggagccaa atgcaggcac tggggcacgc atctggctgg ctaaagaagc gctggaatct
     4081 ggcgattatg atagcgaaga agccttctac aaagcagaag gccgt
//
LOCUS       DAKISA010000214         4087 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.217, whole genome shotgun sequence.
ACCESSION   DAKISA010000214 DAKISA010000000
VERSION     DAKISA010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.217"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..879
                     /gene="adeQ"
                     /locus_tag="OVW45_004322"
                     /gene_synonym="yicO"
     CDS             <1..879
                     /gene="adeQ"
                     /locus_tag="OVW45_004322"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCV5528605.1"
                     /translation="IGLFIALMGLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFII
                     TVLSSRHFHAAVLVSIVVTSCCGLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLEL
                     AGIIFSFMLINLFDSSGTLIGVTDKAGLIDGNGKFPNMNKALYVDSVSSVAGAFIGTS
                     SVTAYIESTSGVAVGGRTGLTAVVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLM
                     TSSLARVNWDDFTESVPAFITTVMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLC
                     VVVVASLFALKIILVD"
     gene            932..1384
                     /locus_tag="OVW45_004323"
     CDS             932..1384
                     /locus_tag="OVW45_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCV5528606.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            1595..2775
                     /gene="nepI"
                     /locus_tag="OVW45_004324"
                     /pseudo
     CDS             1595..2775
                     /gene="nepI"
                     /locus_tag="OVW45_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
     gene            complement(2816..3109)
                     /locus_tag="OVW45_004325"
     CDS             complement(2816..3109)
                     /locus_tag="OVW45_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528607.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3331..>4087
                     /gene="nlpA"
                     /locus_tag="OVW45_004326"
     CDS             3331..>4087
                     /gene="nlpA"
                     /locus_tag="OVW45_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HCV5528608.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQ"
BASE COUNT          900 a          905 c         1077 g         1205 t
ORIGIN      
        1 attggattat ttatcgcctt aatgggatta aaaaatactg gcgttattgt cgccaataaa
       61 gacacgctgg tgatgattgg cgatttaagt tctcacggcg tgttgttagg tattttaggg
      121 ttttttatta taaccgtgtt gtcatcacgt cattttcatg ccgcggtgct ggtttctatt
      181 gtggtgacgt cttgctgtgg attatttttc ggtgatgttc attttagcgg cgtctattcc
      241 attccgcctg atattagcgg cgtcattggt gaagtagatt tgagcggcgc gttaacactt
      301 gaactcgccg gtatcatttt ctcctttatg ctgatcaacc tatttgattc atcaggaaca
      361 ttaattggtg taactgataa agcgggctta atagatggta acggtaaatt ccccaatatg
      421 aataaggcgc tgtatgttga tagcgtcagt tcggtggcgg gtgcgtttat cggcacctcg
      481 tctgttactg cctatattga aagtacttct ggtgtggcag tcggtggccg cacggggctg
      541 actgcggttg tggttggcgt tatgttcctg ttggttatgt tcttctcccc gctggtggcg
      601 atggttcctc cttacgcaac cgccggagcg ttaatctttg ttggcgtgct gatgacttcg
      661 agcctggcgc gcgttaactg ggatgatttt accgaatcgg tgcctgcgtt tattaccacg
      721 gtgatgatgc cctttacttt ctcgatcacc gaagggattg cactcggctt tatgtcgtac
      781 tgcatcatga aagtgtgcac cgggcgctgg cgcgatctga acctgtgtgt ggtggtggtc
      841 gcatctctgt ttgcactgaa gattattctg gtggattagt aactggcgag ggtttccaga
      901 tatcatgagt tctgataacg caggagaact catgatctgg ataatgctcg ccacgctggc
      961 ggtagtgttt gtggttggtt ttcgggtgct gacatccggg gccagaaaag cgattcgccg
     1021 tctcagtgat cggctgaata tcgatgtcgt acccgtggag tcgatggtcg atcaaatggg
     1081 aaagtcagcc ggtgacgaat ttttacgtta tttgcatcgc ccggatgagt cgcacctgca
     1141 aaacgccgcg caggtgttgc tcatctggca aattgtcatt gtcgatggta gcgaacagaa
     1201 cctgctgcaa tggcatcgga ttttacaaaa agcccgcctt tccgcgccga ttaccgatgc
     1261 tcaggtcagg ctggcgctgg gttttctgcg cgaaaccgaa cctgaaatgc aggatattaa
     1321 tgcttttcag atgcgctata acgcgttctt tcagcctgcc gagggcgttc actggctgca
     1381 ttaaaaccgc ttcgtcttat cagcctacag accgcgcctg acctgtcggg tggataagac
     1441 gttcacgccg catccggtaa tctttgccct gttccgtttc gatcttaact ccagctcaag
     1501 caacaccgta aaagttgcaa aaacgttaaa tgcgtcacac atttcaatgt taaagtggtc
     1561 ggcttttccc ctgaaacatg ccacgggtaa caccatgagt gaatttattg ccgaaaaccg
     1621 cggcgcggat gccatcaccc gaccgaactg gtcagccgtt ttctcggtgg cgttttgtgt
     1681 cgcctgtctg attatcgttg agtttttgcc cgtcagtttg ttgacgccaa tggcccagga
     1741 tttaggcatt tcggaagggg ttgccgggca atcggtgacc gtgaccgcct ttgtggcaat
     1801 gtttgccagt ttgtttatta cccagacaat tcaggctact gaccgccgca acgttgttat
     1861 tttgtttgcc gttttgctga cgctctcctg cttgctggtt tcctttgcta actcattcag
     1921 tttgctttta atcggtcgtg cctgtctggg gctggcgctg ggcgggttct gggcgatgtc
     1981 ggcgtcgctg accatgcgtc tggtgccgcc gcgtacggtg ccgaaggcgc tgtcggtgat
     2041 cttcggcgcg gtttctattg cgctggtgat tgccgcgccg ttgggcagtt ttttaggcga
     2101 gcttatcggt tggcgcaatg tctttaatgc ggcggcggtg atgggcgtgc tgtgtatttt
     2161 ctggattatc aaatcattgc cttcactgcc aggcgaaccc tcgcatcaga aacaaaatac
     2221 tttccgctta ttacaacgtc cgggtgtgat ggcagggatg atcgccatct tcatgtcttt
     2281 cgccgggcag tttgctttct tcacatatat tcgcccggtg tatatgaacc tggcgggatt
     2341 cagcgtggat ggcttaacgc tggtgctgtt gagttttggc attgccagct ttatcggtac
     2401 gtcgctttcg tcattcattc ttaaacgttc ggtaaaactg gccttagcag gcgcgccgtt
     2461 aatactggct gtgagtgcgt tggtgctgac gttgtgggga agcgataaaa tcgttgctac
     2521 cggcgtggcg attatctggg ggctaacttt tgcattggtt cccgtcggct ggtcaacgtg
     2581 gatcacccgc tcactggccg atcaggcaga aaaagccggg tctattcagg tggcggttat
     2641 tcagcttgtg gcgcggcaat cggcggttat gcgctggata atattggtct gacttcgccg
     2701 ttgatgtttt ctggcacatt gatgttgctg actgcattgt tggttactgc aaaggtgaaa
     2761 atgaagaaat cctgatatca ggtaaagaag cgttggtcag tagttgttgc tgttcttaca
     2821 tatccgggca ttctacctta cccaacgctt cccaataatt gctctgatta ttacgttcca
     2881 tcgccacgat ggcctcgcgt atatgttgtt tattgttttc atctgacaac attaactttt
     2941 cccacgcttc atctgataat gacgcctgtg gcgtgcaaat tttacgtaaa tcttgtaata
     3001 ccgtggtttt ctcttttgcg gactgcagtg agctaaaagg cgactcggcg tatacgatgc
     3061 ctggcatagc gaaaaaagtg aaaataagca gtagtgtcgt tggtttcatg gattcaacct
     3121 tttacaaatg tgtatcgaat gaagcataag tgtagtttgc tttcatcgct ctcatcatgc
     3181 tgtacgaaac gcgcttgcgt ggtcagtaag aagtgccaga ctttatattc cacttttatt
     3241 cctttttatt cttatcgata gcgtttcgtt ttttaaaccg cagcgacctt accgctatag
     3301 tcaggtaatc attaataaaa ggataaaaaa atgaaactga caacacatca tctacggaca
     3361 ggggccgcat tattgctggc cggaattctg ctggcaggtt gcgaccagag tagcagcgat
     3421 gcaaaacaca ttaaagttgg cgttataaat ggcgcagaac aagatgtcgc ggaagtcgct
     3481 aaaaaggtgg caaaagagaa atatggcctc gatgttgaac tggtaggatt tagcggttcg
     3541 ttgctaccga acgatgcgac aaatcatggc gaacttgatg cgaatgtctt ccagcatcga
     3601 ccctttcttg aacaagataa tcaggcgcat ggttataaac tggtagcggt gggaaatacg
     3661 tttgtcttcc ctatggcggg ttattccaaa aaaatcaaaa ccgtggcgca aataaaagaa
     3721 ggcgcgacag tggcgatccc caacgacccg accaaccttg gtcgcgcact tttactgttg
     3781 caaaaagaga agctaattac tctgaaagag ggaaaagggt tattacctac cgcgctggat
     3841 ataaccgata atcctcgtca tttgcagatt atggaactcg agggggcgca gctcccgcgc
     3901 gtactggatg atcccaaagt tgatgtagcg attatcagca ccacttacat tcagcagaca
     3961 gggctttctc cggtgcacga cagcgtattt attgaagata agaattcgcc gtatgtgaat
     4021 attttggtgg cacgggaaga taataagaat gcagaaaacg tgaaggaatt tctgcaatct
     4081 tatcaat
//
LOCUS       DAKISA010000215         4077 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.218, whole genome shotgun sequence.
ACCESSION   DAKISA010000215 DAKISA010000000
VERSION     DAKISA010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.218"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..786
                     /locus_tag="OVW45_004327"
     CDS             <1..786
                     /locus_tag="OVW45_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCV5528609.1"
                     /translation="IAKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYV
                     NQQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWPGGINITNQL
                     SDTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSESETELQ
                     GNRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVVGQG
                     SRLFIRVDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            799..1758
                     /locus_tag="OVW45_004328"
     CDS             799..1758
                     /locus_tag="OVW45_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCV5528610.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCALTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECADLQETTNAIKVHFLTQNALRFKVGSRHYIINFPFESDGKKIEL
                     PGRSSYNLEEMLSAINFTIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSSCTGNNTN
                     CNSVRFNYNLNTILQVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQG
                     ELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQVGFQLSNDGTNEIQYGKSN
                     YYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
     gene            complement(1796..2695)
                     /gene="nhaR"
                     /locus_tag="OVW45_004329"
     CDS             complement(1796..2695)
                     /gene="nhaR"
                     /locus_tag="OVW45_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCV5528611.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKSFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2761..3927)
                     /gene="nhaA"
                     /locus_tag="OVW45_004330"
     CDS             complement(2761..3927)
                     /gene="nhaA"
                     /locus_tag="OVW45_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCV5528612.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT         1226 a          952 c          860 g         1039 t
ORIGIN      
        1 attgccaaaa cacgtcacca aattaactta tcgaattcga cctcattttc caaagatggc
       61 tattcctcca acaatactgg aattactggc atagccggtg aacatgatca gttaaattat
      121 ggtatatatg ttaatcagca acaacaaaat aatgatacct cgcttggtac gaatttaagc
      181 tggagaactc ccatcgccat aatagatggc agctatagtc attctaaaaa cgcctggcaa
      241 agtggtggaa gtattagtag tggattagtt gtctggcccg gtggtattaa tatcactaac
      301 cagttatccg atacatttgc aattctggat gcgcctgggt tagaaggtgc acatattaat
      361 ggacaaaaat acaatcgaac aaacagcaaa ggccaggttg tttacgacct gataatacct
      421 catcgtgaaa accatctggt acttgatata gcaaacagtg aaagtgaaac agaattgcag
      481 ggcaatcgtc aaattattgc accttaccgt ggagcagttt cttatgtgca gtttacaact
      541 gaccaacgta agccctggta tatacaggca ctgcgtcccg atggttcgcc attaaccttt
      601 ggctatgacg tactggatct ccaggaaaat aatattggag tcgttggcca gggtagtcgc
      661 ctttttattc gcgtagatga aattcccact ggaataaaag ttgctctcaa tgatgaacag
      721 aacttattct gtactattac ttttcaacac gttatcgatg aaaacaaaac atatatatgc
      781 cagtaaaagg agtttactat gaagtggctt ttattaatca ccttgagtct atattctttt
      841 attgtgcaaa gtgctccctg tgcattgacc aatgtcgggg aacaacgtgg aacatatata
      901 ctcaagccac tgagtatgaa aggtaatctc accgccgaaa aaattgaaaa caccggaaat
      961 atcattgagt gtgcagactt acaagagacg accaacgcca ttaaagttca ttttttaaca
     1021 cagaacgctt taaggtttaa agtcggaagt cgtcattata ttattaattt cccctttgaa
     1081 tctgacggta aaaaaataga gcttccagga aggtcatctt ataatttaga agaaatgcta
     1141 tctgcgataa atttcaccat tccatactct atcgcatctg tatcagatag ttccaacacc
     1201 gcagatattt ttccaggaac tccttttcct ttgttgttcg aaattgaagt gagttcatgt
     1261 acagggaata atactaattg taatagcgtc cgctttaatt ataatttaaa tactatttta
     1321 caggtaaatt taatgacctg tggttttgaa gatcaatcca tcgacactgg acgatttgta
     1381 ctgtctcgta tcaataatga aaattatcag tttcatcata taagatttga ctgcatccag
     1441 ggcgagcaag gtgaattagt ttttgaaccc agtgatgttg aatattactt tgaaccagtg
     1501 acgatacagg agtcaggaac cagcatatta aaaaatgagt tagaaaacag tgaggacggt
     1561 gcgggtcaag taggctttca actctccaat gatggtacga atgaaatcca gtatggtaaa
     1621 agcaattact actcttttca gcatccccat gaaggaagta atcagattcc tctatttatt
     1681 cgcccgcgta catatggaaa taatgtctca agcgggcaaa taatgagccg agtcaaaatc
     1741 gtggttatgt acaactaatc tatctccccc accttaactt tgggggaggt gccgattaac
     1801 gcgccgctgg actaaaaagc gcagaataat ccgtattgca gattcgctgt accgccgggt
     1861 gctgaatcat ccgctcagca aaaatagcat ggtactcttc catcacattc tcgacgcgac
     1921 caatttctac gacagtttta tcggcataaa agtcatatgc ataaagcgtt ggggcaacga
     1981 agattgcatt gtgcatcgca ccaaaagctt tcatcaacgc ggcatcatca aactcgccga
     2041 ggatttctac gtttaatccc tgggagttaa accagttaag caatttgcgc cctaacattg
     2101 aacgtcgccc aggaatcaaa agtcgccgtt cttccagaca agccgggaac gatttttctg
     2161 gtggtggatt tgtacaccag aaactcacgc cacattcgcc aattctcacg gagaacaggc
     2221 cttcctgctg cgtagagtct atcggacagt cagaaatgat catatccagt ttatgctgac
     2281 ttaattgctc cagcagcatt tcgtgggtgg attcgaagca gcgaagatga atgggctcgc
     2341 cttctaccac tgcggcgtta agtacgctac tgaccaggcg tttggaaagt gcatcagcca
     2401 cgccaacgtc aaacaataaa ttggattctt tgcgatagtt cacaatatcc agcatttcct
     2461 ggcttaaggt gaacatttta tcggcatagc gatagaccag ttctcccagc tcgctgggtt
     2521 cgagaccacg tcccttgcgt ttaaataatt tgccttgcag gcgctcttcc agcgctcgaa
     2581 tctgtccggt aatggtttgt ggagttaaat aaagcgcctc cgctgcgcca accacggaac
     2641 cttctttata gacatgccag aagtaataca agtggttgta attgatatga gacatgctca
     2701 tttctctccc tgataacaat gaaaagggag ccgtttatgg ctccccggta aaccgtcctg
     2761 tcaaactgat ggacgcaaac gaacgcgtaa ccagctgtat ccaattaccg ccgaagagat
     2821 agaaccgacc aggataccga gtttcgccca gttaatcagt tctggatcta cgctaccaaa
     2881 ggccaggctg gcaataaaga tagacatagt aaaaccgata ccgcacagga tccccaccgc
     2941 cataatttgc tgataagtcg ttccctcagg cagatgcgcc agtttcaaac gcagcgccaa
     3001 ccagcagaac aaactaatcc ccagcggttt gccaatcagc aagccagcga tgatccccaa
     3061 tggcagaatg gaggtcaagc catccagcgt gacgccttgc agtgaaacgc cagcattagc
     3121 aaatgcaaac agcggtaaaa tcagatacgc cacccacggg tgcaatacat gctccagtcg
     3181 cttcgctgga gaacgcccat gcttctcttt caaaggaata aagaagccga caattacccc
     3241 cgccagggtt gcgtgaaccc ccgatttcaa caccgcagtc cacaacacca cgccaacaag
     3301 aatatagacg cccgtgcggc gtacaccaca cagattcaat accgcgagta ccgcaattgc
     3361 tacagccgcg acgccaagag aggccatcga taagtcatta gtgtagaaca atgcgatgat
     3421 aatgatggcc ccaagatcgt cgataatagc cagagccatc aaaaagatct tcagtgctaa
     3481 cggaacacga cttcccaaca gcgccagcac accaagtgca aaggcaatgt cagtagccgc
     3541 cgggatcgcc cacccttcgc gggtaatcgg atcggcatag ttaaaggcca gatagagtaa
     3601 tgccggaaca atcatcccac caatagcggc gataaccgga aatgcggcct ggcgtaagct
     3661 ggctagcgat ccttgcatca gttcacgttt aacttccaga ccgactaaca ggaaaaatac
     3721 cgccatcagc gcgtcattta tccataacag catgtttttg ttgatttcga gtgaaccaac
     3781 ccggagctga accggcgtct ccagaaagtc gtgataccat ccactggttg cgccgctgtt
     3841 ggccatcatc atcgccagga tagcggcaat gataagaata atgcctcccg aggcatcact
     3901 gctaaagaat cgatgcagat gtttcacttt ttatttctct ttcaggtgaa tagatcgttt
     3961 ttactatttt accccgcaca aatcatcgtt aaaatcagat tatagtgtag gaataactcg
     4021 ttttttacga gcaattaaag gatttgcgcc atcagcggtg agatgagttt ttgacgg
//
LOCUS       DAKISA010000216         4040 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.219, whole genome shotgun sequence.
ACCESSION   DAKISA010000216 DAKISA010000000
VERSION     DAKISA010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.219"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            926..1642
                     /gene="nanC"
                     /locus_tag="OVW45_004331"
     CDS             926..1642
                     /gene="nanC"
                     /locus_tag="OVW45_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCV5528613.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            1662..2768
                     /gene="nanM"
                     /locus_tag="OVW45_004332"
     CDS             1662..2768
                     /gene="nanM"
                     /locus_tag="OVW45_004332"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCV5528614.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSLAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2833..3813
                     /locus_tag="OVW45_004333"
     CDS             2833..3813
                     /locus_tag="OVW45_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCV5528615.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPNNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         1217 a          845 c          914 g         1064 t
ORIGIN      
        1 ggagataagg gggaacgcag gagacgcttt ttcatctaca ttcgtgggta aatatggctt
       61 tggtcactgc gacttaaacc ttcctcaatc acagctattc gccgggcgat acggcaaaca
      121 aactaagtga aacatcatcc tgaaaacacc aacatcaaca agcctctcta gatcgacttc
      181 agaagcgacc agttacaagc cacaaatcag gaaacatatt tcatggtgag aattatgatc
      241 agcctgatgg cgggtaaaca cttgttttca ccacgctcac tgagttagct tgcaaagctt
      301 cccttgttta gaccctaaat aaatttttta taaaacaaac agttaaaaga aatcctttag
      361 tttttgaaga ccacctcaag tgttcgtctg gcttcacatg gcatcttcct cttagaaaaa
      421 gatcgacata ttttgtgaca cgaattgcaa atctcgtttt gttgtatggc ttgcgtgatt
      481 tttgatctgg tataacaggt ataaaggtgc gccgcgatag tcaatgagac agggcatctc
      541 gcaatctatg gcaaacatca cttcagttct ttctcatcgg gtgatgaaaa cgcacttcag
      601 tctgaaagga atatgaaaat gagatcaaca gacactctat tttatgactc tgggtaaaat
      661 ggattgagta agtgatatag cttacgaaca ttcaaatcaa ttaaacatca gaagagattt
      721 tatactcagg tatttaatct gattctctgt ttatttaaat aatgtggaaa gagaattttc
      781 acaggagacc ttatacaaaa atataaaata agctacccgt tgccaaagac actataagcc
      841 tggcaaaaaa tattacacaa cataaatgct aattgtttat gcgggctttg tattgctttc
      901 tgtatcctac aaatgagtga aatttatgaa aaaggctaaa atactttctg gcgtattatt
      961 actgtgcttt tcgtcaccat taatttctca ggctgcgaca ctggacgtac gtggtggata
     1021 tcgtagcgga agccatgcct atgagactcg actcaaagtc agtgagggat ggcaaaatgg
     1081 atggtgggca agcatggaaa gtaatacctg gaataccatt catgataata aaaaggaaaa
     1141 tgccgcactc aatgatgttc aggttgaagt taattacgcg attaaacttg atgatcaatg
     1201 gacggtgcgc ccgggaatgt taacgcattt tagcagcaac ggcacacgct acggacccta
     1261 cgtaaaactg tcctgggacg cgacaaaaga tcttaatttt ggcattcgct atcgttacga
     1321 ctggaaagct taccgacaac aagacttatc cggtgatatg tctcgtgata acgttcatcg
     1381 ttgggatgga tatgtcactt accatattaa tagtgatttc accttcgcat ggcaaacgac
     1441 gctatacagc aaacagaatg attatcgcta tgcaaaccac aagaaatggg cgacggaaaa
     1501 tgcctttgtt ctacaatacc atatgacgcc cgatattacg ccatacatag aatatgacta
     1561 ccttgaccgc cagggtgttt acaacggcag agataattta tcggaaaaca gttatcgcat
     1621 tggtgtgtca tttaaactgt agtagacagg agacagtcac aatgaataaa actataatgg
     1681 cgcttgctat catgatggct tcatttgccg caaacgcgtc tgtattaccg gaaactcctg
     1741 tgccatttaa aagtggtacc ggagcaattg ataacgacac tgtctacatt ggtttaggta
     1801 gcgcaggtac agcatggtac aagctagata cacaggccaa agataaaaaa tggactgcgt
     1861 tagctgcatt ccctggtgga ccgagagatc aagcaacctc tgcatttatt gatggcaatc
     1921 tatatgtgtt tggcggcatt ggcaaaaaca gcgagggatt gactcaggta tttaatgacg
     1981 tacacaaata caaccccaaa accaatagct gggttaaatt gatgtcgctc gcgccgatgg
     2041 gcatggcggg tcatgtgact tttgtacaca acggcaaggc ttatgttact ggcggtgtta
     2101 accagaatat cttcaatggc tattttgaag atctcaacga ggctggaaaa gattcaaccg
     2161 ctatagataa aatcaacgcc cactattttg acaaaaaagc agaagattat ttcttcaata
     2221 agtttctgtt gtcttttgat ccctcaacac agcaatggag ttacgctggc gaatcgccct
     2281 ggtacggaac ggctggtgcg gcggttgtga ataaaggtga taaaacctgg cttattaatg
     2341 gcgaagccaa accaggattg cgaacggatg ccgtatttga acttgatttc accggtaata
     2401 atttaaaatg gaataagctt gatcccgtct catcaccaga tggcgtcgct ggcggttttg
     2461 cggggataag caatgattct cttatatttg ccggaggggc cggattcaaa ggttcacgag
     2521 aaaattacca gaacggtaag aactatgcgc atgaaggcct gaaaaaatca tatagcactg
     2581 atattcatct ttggcataac gggaaatggg ataaatcggg tgaattatcg caaggtcggg
     2641 cctacggagt atcattgccc tggaataata gtttattaat tattggcggt gaaactgcag
     2701 gcggcaaagc ggtgacggat tcagttttaa tttctgtgaa ggataataaa gtcacagtac
     2761 aaaactaacg cttcagggcc ccggtaaagg ggccctgcta tcaatttgtc atttgtatta
     2821 cgcgaaaatt atatgaacgc aataatatcg cccgattatt actatgttct taccgttgct
     2881 ggtcagtcta atgccatggc gtatggcgaa ggactgccat taccggacag ggaagatgcg
     2941 cctcattcca gaattaaaca attagcgaga tttgcgcata cgcatcccgg aggcccgcca
     3001 tgtcacttta acgacattat tccactgact cactgcccac acgatgttca ggatatgcag
     3061 ggttatcacc atcctctggc aacgaatcat caaacacagt acggcaccgt tggccaggca
     3121 ctgcatattg cacggaaatt actgcccttc attcctaata atgcagggat tcttatcgtt
     3181 ccgtgttgcc gtggcggatc ggcttttacc gcgggcagcg aagggacata ttcagaacgg
     3241 cacggagcca gccatgatgc ttgtcgttgg ggaacggata ctccgctgta ccaggattta
     3301 gtcagcagaa cgcgagccgc actggcaaaa aatccgcaga acaaattcct cggcgtatgc
     3361 tggatgcaag gcgaatttga cttaatgacc agtgactacg cgtcacaccc tcaacacttt
     3421 aatcatatgg ttgaagcctt tcgtagggat ctaaaacaat accattctca gcttaataat
     3481 attactgacg cgccatggtt ttgcggcgat accacctggt actggaaaga aaatttcccg
     3541 cattcgtatg aagctattta tggcaattat caaaataatg ttttagccaa tattattttc
     3601 gtcgacttcc agcaacaagg tgaaagagga ctgacgaacg cgcctgatga agacccggac
     3661 gatttaagca cgggatatta cggttcagcg taccggtcac cggagaactg gacgacggca
     3721 ctgcgaagca gtcatttcag cacggcagcc cgtcggggga ttatttctga cagatttgta
     3781 gaagcaattt tgcagttttg gcgcgaaagg tgagcgggga gtttattcat gggggatgtc
     3841 cttgttcgtt ctcagagtag acaacaaaaa acaaataaga tcaaggtaat atctcttcac
     3901 ttgcgggctc gaacagcagg caattgccaa tataataatc tggcacaagg gtgaaaaacg
     3961 cgcaccgcat aaaccgttgt tattgcttac agattagccg gatgccttaa tagacatccg
     4021 cgccttttcg attatggctc
//
LOCUS       DAKISA010000217         4024 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.220, whole genome shotgun sequence.
ACCESSION   DAKISA010000217 DAKISA010000000
VERSION     DAKISA010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.220"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            172..507
                     /locus_tag="OVW45_004334"
     CDS             172..507
                     /locus_tag="OVW45_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCV5528616.1"
                     /translation="MLSAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTED
                     TIDGLKEFDKYKTRILDSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLT
                     LSYREVNRD"
     gene            500..982
                     /locus_tag="OVW45_004335"
     CDS             500..982
                     /locus_tag="OVW45_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528617.1"
                     /translation="MIDYKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIF
                     NDQGRANLTIDFMYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTS
                     KKVNKFEIMDQVDDDRLAQLIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR
                     "
     gene            complement(1016..1096)
                     /locus_tag="OVW45_004336"
     CDS             complement(1016..1096)
                     /locus_tag="OVW45_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528618.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(1132..1556)
                     /locus_tag="OVW45_004337"
                     /pseudo
     CDS             complement(1132..1556)
                     /locus_tag="OVW45_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1986..2476
                     /locus_tag="OVW45_004338"
                     /pseudo
     CDS             1986..2476
                     /locus_tag="OVW45_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2701..3192
                     /locus_tag="OVW45_004339"
     CDS             2701..3192
                     /locus_tag="OVW45_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCV5528619.1"
                     /translation="MSTETIEIFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSE
                     DNG"
     gene            3527..3985
                     /locus_tag="OVW45_004340"
     CDS             3527..3985
                     /locus_tag="OVW45_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001426852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCV5528620.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPQKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKIDG"
BASE COUNT         1505 a          648 c          648 g         1223 t
ORIGIN      
        1 aagatccata atgacattaa catacaaaat catgtaataa actcgtcaac agacgcacat
       61 acacacaatg ccccacctgc cataaaagtt aatgcgggat acaaagagag cattggtggc
      121 gcaaagaatt tcaataacaa aatcctcaaa catataaaaa gtcatctaat tatgttgagc
      181 gcatttgtca taggcgcata ttctgcatat tggctatgga ataataatca accgaagcca
      241 tttttcaagg attacaagac tgttgcggaa attaatggct gccattttaa tgtaacagaa
      301 gatacaattg atgggttgaa agagttcgat aaatataaaa cacggatact ggattcgggg
      361 attaactgta aaaaatatcc gtggttatac ttccctcttg caaaatcctc accggggatg
      421 attgttatgg catgcaataa aaactataac caacatgaag tggcagactg tttaaccctg
      481 tcttaccgtg aggttaatcg tgattgatta taaaaaaaac ttattattta ttttagtttt
      541 tatcagtggg tttattctct tcactgtcta ttcttacact gcagaaaaaa tgatatataa
      601 cgaaacatgc actgcaaatt gggtaatttt taatgaccag ggacgagcaa atcttaccat
      661 cgactttatg tataacaaaa aaaataaaac aggaactgtt gcgttgagtg gaacctggca
      721 acaaggtaac agagaaagta aatcaatacg gcgaaatatt gaatacacat ggattgaaaa
      781 ctatgacaca gcccatttaa catcaaagaa agttaataag tttgagatta tggatcaagt
      841 tgatgatgat agacttgcac aacttattcc tgatttttat gtctttccag aaaaaagtgt
      901 aagctataat atactaaagc aaggtaagca tgcttttatt ttgagcattg gtaacagagc
      961 aataatgcat tgtgcaaggt aaactaaaat agataacaaa aaagatggaa cctcgttaat
     1021 cataaaacaa taaaattaaa tattctcgca gtatatggca gtctaaagca tcaaagtttt
     1081 gatcaacatc tttcatttta gacatctcct tgcaatgtaa aatatcatgt atcaacaaca
     1141 tctggtgcaa atccatgagt cgaactcgac attcatctta gctcgattat tattccttcg
     1201 tacagtcgat gtaaacaata cagaaagagg attattaaga accagtttac tcaggaatca
     1261 atagaggaaa ggactaacgt ttctttaaaa gaattgattt catcatctgt taaactaaac
     1321 tcatcattga cagatcgtga gatataactg tttttaactt tactctttac gttgacttta
     1381 ttgacagaat taacattcac atatcttgaa tttaatgtcc atgatgttgt ttgcgtgaga
     1441 acattctcag cattaaggaa tttttttaac ggaatccttg gtttcttttt cgagtccgaa
     1501 tttacaatat catgcatata gaccatttca tgaatctgcg aaaactcaat gtccattgta
     1561 tacctcacat ttttaccgtg actcgatgtt actgttcaat aatcaccttc catcaatact
     1621 aaaattaata cccctaatgt gccgataaca aatatagtca ttctacgtaa cgtctccata
     1681 aggtgataac cttgatcaaa tgaatttcga atataataaa ggaaatcttt tgagatgttt
     1741 atcacgaata aagctattta tgttaccaat atcatttcga gtgcgcacac tatctatctg
     1801 ttttttcgag atataaaaaa caacgtgtaa gttatgtaaa ttgccgatgc tactgagtaa
     1861 cattatggta aaaaaagtaa aaatgagcga tttttgatag aataaagcat gagaaaaggt
     1921 gatatttgac attatcagaa gctgcgaatt cggattttgc tctaatcaaa tgagagaaat
     1981 gtaaaatgaa gccacgaaat attaataata gcctaccact gcaaccatta gttcctgatc
     2041 aggagaacaa aaataagaaa aatgaagaga aatccgttaa tccagttaaa atcacaatgg
     2101 ggtctggttt aaattatatt gaacaagaat ctcttggagg aaaatatcta acacatgatt
     2161 tgtcaataaa gatagcggat atttctgaag agataattca gcaagcaata ttatctgcta
     2221 tgagcatata taaattttcg ataacagatg atttaatgag tatggctgta aatgaactca
     2281 taaaactgac caaaatagag aataatgtag acctgaataa attcactact atatgcacag
     2341 acgttctatc ccccctcgtc accagacata ataaagaaaa aaaacaaacg acattctacc
     2401 cttctcaaaa atcccctttt taattttcat tgaaaaaatg gcaattgata ctatgaatcc
     2461 aggtcataca cattaaggca tttatgtaat gtcgtaatta agataactaa taaggtgaat
     2521 attagtaaaa caactaatga tgttattagt ttgtagtgaa cagtactttt accaataatg
     2581 aaaaatatac cataagcaat gcaagcttaa aactaataat ttaaaaaaga taaatgtaaa
     2641 aatattaatg agtcaatcaa taataattta gcatcacgag acacatcaca gaggaatatt
     2701 atgagcacag aaacaattga aatattcaat aatagtgatg aatgggcaaa tcaactaaaa
     2761 cacgcattat cgaaaggaga aaatctggca ttactacatg gtttaactcc tgatatcctt
     2821 gatagaatat atgcatatgc attcgactac catgaaaagg gtaatataac ggacgcagaa
     2881 atttattata aatttttgtg catttatgcg ttcgaaaatc atgagtatct aaaagatttt
     2941 gcatcagtat gtcagccaaa aaagaaatat caacaagcat atgaccttta caaactaagt
     3001 tacaattact ccccgtatga tgactattca gttatttatc gtatgggtca atgtcagatt
     3061 ggggctaaaa atatcgataa cgcaatgcaa tgtttctatc acattattaa caattgtgag
     3121 gatgatagtg ttaagagtaa agcgcaggca tatattgaac tcttaaacga taattcagaa
     3181 gataatggct aagcattaca atcatttggt taaatttgct ggttatagtg cagttaacta
     3241 ctataaccag cacctcttat gttttcttaa atcagcatct taaaacagtt aagagcatac
     3301 tataaatcat tatttttcaa tgtgttagag aacaatctac tttataaaaa ttgaataagg
     3361 ctatttcaga ttacatcact gaattcctaa gataaattag tgaacacaac catagatatg
     3421 tcgaatgtat tttcctgtag cattaatgac acataaatat gaatagccat aatttctatt
     3481 gcttaagcag gatgcaagaa accaattttt tcatagaggt taactaatgg acttagaaaa
     3541 taaattctca tatcattttc ttgagggatt aacgctcacg gaagatggaa ttcttactca
     3601 aggaaatgag caagtttata ttccacagaa agagttaggt gtattaatag tattacttga
     3661 atccgctggt catgttgtac tgaaagatat gatcatcgaa tcagtatgga aaaatattat
     3721 tgttagtgac gagtccctga caagatgtat ctattctttg cgctgcattt ttgaaaaaat
     3781 tggctatgat cgttgcatag aaacaatcta ccggaaaggt tatcgtttca gcgggcaggt
     3841 tttcaaaact aaaattaatg aagataatac ttcagactat tccatagcta tattcccttt
     3901 cactacttca ttgaatacac tggatccatt aatacttaat caggaattag tgcaaatcat
     3961 ttcaaataaa aaaatcgatg gttagactgg ccccctgaat ctccagacaa ccagtatcac
     4021 ttaa
//
LOCUS       DAKISA010000218         4002 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.221, whole genome shotgun sequence.
ACCESSION   DAKISA010000218 DAKISA010000000
VERSION     DAKISA010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.221"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..1255)
                     /locus_tag="OVW45_004341"
     CDS             complement(<1..1255)
                     /locus_tag="OVW45_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCV5528621.1"
                     /translation="MAQFKVDDFLIELSFNSQKVLKGLEKAEKQTMQVASRIEKRLNK
                     SFRVNPTPLNDSLKLMERNVDKTVSKIEQRLKNTRAFKIKTEIEDTLKPLRQPRQPRI
                     SGSRAITAAYSANMSKLRDFNPMLEKYFKSQYYSLSSKSKTLGNETFNRELAKLNQSL
                     RETLSKFNKTTSKNNHSENASNSLDVLATSAIKAGTAIYSFQTALEAYRRIMEVGLKK
                     EASQRAAQFVLGDEGAKRATEFVKNLADSSGVDQIETLSSFAKFSAGAGDMNADQKES
                     LFSNVIGTSRLMGLSTDEINGILKAFEQMASKGKIQAEELRGQLGDRMAGAFQLFARS
                     LGMTTEELDKAMKDGKVLSKDVLPKVSAEMGRMIDKAGGWEKIINSTQTQLGRLSNSW
                     NNNLALMFDGSQEGLTDFTRSLTNLL"
     gene            complement(1412..1621)
                     /locus_tag="OVW45_004342"
     CDS             complement(1412..1621)
                     /locus_tag="OVW45_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528622.1"
                     /translation="MFEERLETIRSVCENLKLQTKPALRIKNKRQVITSHKPKTRKIP
                     KWCIDRIPSDAQIIGETELHYLVRH"
     gene            complement(1644..2354)
                     /locus_tag="OVW45_004343"
     CDS             complement(1644..2354)
                     /locus_tag="OVW45_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCV5528623.1"
                     /translation="MTNDLYINGKKVDSFGDWSPQGEHPLHTIQRQEHDKRIELEQEL
                     KMSPKVISFVSPEPQEMPDICKTEALFELEREYYPALKSQRLRLDVAYDAVKNFEETS
                     KPSEYDIAYEIKSNPFIYYEGNFNDGFGTAIEDVPKVLQSIPEGFRLIDVRQALRGSG
                     QFILMTDKTDEEILQLAEANLTKHYSGKLDKLKQKLKKELDTMKELVSEYESQKKIAL
                     MADIEQLTKISQKYSKAV"
     gene            complement(2869..3192)
                     /locus_tag="OVW45_004344"
     CDS             complement(2869..3192)
                     /locus_tag="OVW45_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528624.1"
                     /translation="MNEYELQIFIDSDTAMMVQAFADSGVSIDLDKLLKLMAENSEEI
                     EDFIQEIETGEPRFMFPVTDSNMKRLIIEETNKYSVSPEKYLKAAIAILYSDNILVTD
                     SKVVH"
     gene            complement(3371..3571)
                     /locus_tag="OVW45_004345"
     CDS             complement(3371..3571)
                     /locus_tag="OVW45_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528625.1"
                     /translation="MLCIKFEYLTDKMIKHVSDLLIKEGGFGDACNPKDIFIHATSPN
                     ATLKTAVTAEWFERNKAELGYW"
     gene            complement(3571..3843)
                     /locus_tag="OVW45_004346"
     CDS             complement(3571..3843)
                     /locus_tag="OVW45_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000816065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCV5528626.1"
                     /translation="MKQWRDDIKRFFATYFMINYETGMLEWIDGGEVKTRDDAYGNPM
                     IRYGTRDYYLKYVIWFLHHEVQATKKIIFRDGNKRNCHPNNLLLEI"
BASE COUNT         1168 a          845 c          654 g         1335 t
ORIGIN      
        1 taagtaaatt agtcagtgac cttgtaaagt ctgttaaacc ttcctgtgaa ccatcgaaca
       61 ttaaggccag attattattc catgaattac ttaagcggcc taactgcgtc tgagtagagt
      121 taataatctt ttcccatcca ccagctttat caatcatcct tcccatttca gcggatactt
      181 taggaagaac atctttagaa agaaccttac cgtctttcat ggccttgtct aattcttccg
      241 tagtcatccc aagacttctg gcaaataact gaaaagcacc tgccattctg tcacctaatt
      301 gaccgcgtag ctcttcagcc tgaatctttc ctttagatgc catctgctcg aaagctttta
      361 aaatcccgtt aatttcatcg gtgcttagcc ccattaatct ggatgtacca atcacattag
      421 aaaataagga ttctttctgg tctgcattca tatcaccagc accagcggaa aacttagcaa
      481 aactgcttaa tgtttcaatc tggtctacac cactactatc agcaagattc ttaacaaatt
      541 ctgtagctcg tttagcacct tcatcaccta aaacgaattg tgccgctcgt tgtgatgctt
      601 cttttttcag accaacctcc ataatccgcc tgtaagcttc taaggctgtc tgaaaactat
      661 aaatggctgt acctgctttg attgcactcg tagctaatac atccagacta tttgaagcat
      721 tttcactgtg attatttttg ctggtggtct tgttaaactt agaaagtgtt tcacgaagag
      781 actgatttaa ctttgctaac tcacgattaa aagtttcgtt tccaagtgtc ttggatttac
      841 tgcttaagct gtaatactgg cttttaaaat acttctcaag cataggatta aagtcacgaa
      901 gcttgctcat attcgctgag taagctgctg tgattgctct gcttccgcta attcgtggct
      961 gtcttggttg tctaagtggc tttaaagtat cctcaatttc agttttaatc ttaaacgctc
     1021 tagtgttttt aagacgctgc tcaatcttgg aaacagtttt atcgacattt ctttccatta
     1081 gtttgagaga atcatttaaa ggcgtaggat taactctaaa cgacttattc aatcgcttct
     1141 caattcgtga tgctacctgc atcgtttgtt tctcggcttt ttccaagcct tttaaaactt
     1201 tctgagagtt aaaacttaac tcaatcagaa aatcatcaac tttaaattgt gccattcata
     1261 tttttcacct ctttaagcgt gttgtacgta aattctattt ttcagtgtgg gtattagctt
     1321 tacagatttc acgtagcaca catacaaaaa aagggagagc taccgatgtc actcgataat
     1381 ctctccccgt atttacccaa ccaattcttt tttaatgtcg gacaagataa tgtaattccg
     1441 tttcacctat gatttgagca tcagaaggaa tacggtcaat acaccacttt ggaatcttgc
     1501 gtgtttttgg tttgtgtgag gtgataactt gacgtttgtt tttaattctt aaggctggct
     1561 tagtttgtaa ctttaagttt tcacaaacag accgaatagt ctctaaacgt tcttcaaaca
     1621 ttaatcacct ctttaattca taatcaaaca gccttgctat acttctgaga aatcttcgtt
     1681 aactgctcaa tatcagccat caaagcaatc ttcttctgcg actcatattc actaacaagt
     1741 tctttcatag tatcaagttc ttttttaagc ttttgcttta acttatctag tttaccggaa
     1801 taatgcttag tcaaattagc ttcagctaac tgaagaatct cttcatctgt tttatcagtc
     1861 atcagaataa attgaccaga accccttaat gcttgtctga catcaattaa ccggaaccct
     1921 tcagggatac tttgaagaac tttaggaacg tcttcaatcg ctgtaccaaa accatcatta
     1981 aagtttcctt cataatagat aaacgggttt gacttaatct cgtaagcaat atcatattct
     2041 gaaggtttcg aggtttcttc gaagttctta acagcatcgt aagcgacatc aagtctaaga
     2101 cgctgtgatt ttaaagctgg gtaatattca cgctcaagct caaatagtgc ttcagttttg
     2161 cagatatcag gcatttcttg aggctcagga gacacaaagg aaattacctt tggtgacatc
     2221 tttagctctt gctcaagctc aatgcgcttg tcatgctctt gacgctgaat agtatgtagc
     2281 ggatgttcac cctgtggtga ccagtctccg aagctatcaa cctttttacc gttaatatat
     2341 aaatcgtttg tcattataat ttcctctaag tgtggttatt tcttaataga gcaccttcaa
     2401 cgccaataca cttagaagat gacttaacga tttattaaag atgctctatt aagaaaagcc
     2461 ctaaacataa tctgtgaagg gctttggagg tttgcttata acgttaacga agtttcagga
     2521 aaactttctt taaaagctct gtttaaaaac tcttaaagaa agccctaact catgattagg
     2581 gcaaagctgt aaaggattca ataatgaatg aacaaagact ttttgagata gcctctcaga
     2641 ccaacctgaa aggctaggac gaaaagcatt gcacctttcc aactcagaat atccatccat
     2701 cggattattg aaagaaaaaa taagagagaa ttgctctccc catatataca attatgtcat
     2761 gctcaaggtt gtttgtcaac agcttttaac agattggtaa catttatact ttaatagatg
     2821 cacctgcaat cataccaatc aatacaaaac agataatcac caattccatt aatgcaccac
     2881 cttcgaatcc gtcaccagaa tattatcgct atacaaaatt gctatagccg ccttgagata
     2941 tttttcaggg ctaacgctgt atttgttcgt ttcttcgata ataagacgct tcatgtttga
     3001 gtcggtaaca ggaaacataa aacgcggttc accagtttca atttcctgaa taaagtcttc
     3061 aatttcttct gagttttcag ccattagctt tagtaattta tcaaggtcaa ttgatacacc
     3121 ggaatcagcg aaagcctgca ccatcatagc agtgtcgcta tcaatgaaaa tctgtaattc
     3181 atattcgttc atttttaaaa tctccttttt accagacctc ctcaatcgct ctgtaacgag
     3241 ctaaacagtc tttttcaggg gtagcctgta cagtggtatt acccacccta aaataagata
     3301 gttttttgcg ttagaatgcg attgagagcg tctgataagc tgtttttcta atttctctta
     3361 aagtctcgga ctaccaataa cctaattccg ctttatttcg ttcaaaccat tccgctgtaa
     3421 ctgctgtctt tagtgttgcg tttgggctgg tggcatgaat gaagatatct tttggattgc
     3481 aagcatcacc gaaaccacct tctttgatga gcaggtctga tacatgctta atcattttat
     3541 ctgtcaaata ttcgaattta atacataaca ttaaatctcc aggagtaaat tgtttgggtg
     3601 gcaatttctt ttattaccat ccctgaagat aatcttttta gttgcctgaa cttcatgatg
     3661 caaaaaccag atgacatact tcagataata atcacgagtg ccataacgaa tcattggatt
     3721 accgtaagca tcatctcttg tcttaacctc tccaccatca atccattcaa gcattcctgt
     3781 ctcataatta atcatgaagt aagttgcaaa gaaccgttta atatcatctc gccattgctt
     3841 cataatcaga cctcctgaga cagtttgtga attttgatac ttagtaacct tgctaaatcc
     3901 agtccaaaag catttaagga tttcagacta tcaaggtcat cttgcttaat cctgatatgg
     3961 tgtcttgcag cctctaagcg actattaagc aaatcctgtt cg
//
LOCUS       DAKISA010000219         3949 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.222, whole genome shotgun sequence.
ACCESSION   DAKISA010000219 DAKISA010000000
VERSION     DAKISA010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.222"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..125)
                     /locus_tag="OVW45_004347"
     CDS             complement(<1..125)
                     /locus_tag="OVW45_004347"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Involved in the nonphosphorylative, ketogenic
                     oxidation of glucose and oxidizes gluconate to
                     5-ketogluconate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HCV5528627.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGYLLATGLGKYGAQIIINDIT"
     gene            complement(149..1180)
                     /gene="idnD"
                     /locus_tag="OVW45_004348"
     CDS             complement(149..1180)
                     /gene="idnD"
                     /locus_tag="OVW45_004348"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HCV5528628.1"
                     /translation="MQVKTQSCVVADKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQADELQGKRVFVSGVGPIGCLIVSAVKTLGSAEIVCADVSPRSLSLAKEMGADVL
                     VNPQNDDMDHWKAEKGHFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYLFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            1397..1960
                     /gene="idnK"
                     /locus_tag="OVW45_004349"
     CDS             1397..1960
                     /gene="idnK"
                     /locus_tag="OVW45_004349"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCV5528629.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQDRICAKGGSASDQRCE"
     gene            complement(1964..2983)
                     /gene="ahr"
                     /locus_tag="OVW45_004350"
     CDS             complement(1964..2983)
                     /gene="ahr"
                     /locus_tag="OVW45_004350"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCV5528630.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYEPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRNGKARYRVV
                     LKADF"
     gene            3179..3263
                     /locus_tag="OVW45_004351"
     tRNA            3179..3263
                     /locus_tag="OVW45_004351"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3213..3215,aa:Leu,seq:caa)
     gene            3449..>3949
                     /locus_tag="OVW45_004352"
     CDS             3449..>3949
                     /locus_tag="OVW45_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCV5528631.1"
                     /translation="MALTDIKVRTAKPTDKQYKLTDGGGMHLLVHPNGSKYWRLQYRY
                     EGKQKMLALGVYPEITLADARIRRDEPRKLLANGVDPGDKKKSDKVEQSKARTFKEVA
                     IEWHGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEV
                     AARLQQR"
BASE COUNT         1047 a          958 c          923 g         1021 t
ORIGIN      
        1 gtaatatcat taataattat ttgtgcgcca tatttaccca ggccggttgc cagtaaatag
       61 ccaatgccct gtgctgaacc ggtaatcaag atattttttc ctgccagtga aaatagatcg
      121 ttcattgtat tattccttta tttatctctt agaaaacaag ctggactttt gctgcctggg
      181 ttttatcacc ggcgaaacgt agcgcctctt ccaggtcagt gaagagatat tcagcactca
      241 gtaaaggcag tggattgata acgccattcg ccagccatga cactgcggta ttaaattcgc
      301 tggtaaaacg gaaagagcct ttgagtgaaa tctccttacc aatcaacgtc atcattggga
      361 attctgccat cgcgcctccc atccctacct gcaccattac gccgcgtgca cgagtgacct
      421 ccagacaggt attcactgat gaaggatgac cggacacttc aaagctgaca tcgaaatggc
      481 ctttttccgc tttccagtga tccatgtcgt cgttttgtgg gtttacgagc acatccgcac
      541 ccatctcttt ggccagcgaa agggaacggg gactcacatc ggcacagaca atttccgcgg
      601 accccagtgt tttcacagca ctgacaatca ggcagccaat gggcccaaca ccggaaacaa
      661 atactcgctt gccctgtaac tcgtcggcct gatgtgcggc atgaatcgcg acggctaaag
      721 gttcggcaaa agccataacc ttttcgtcag ctttggccgg ataagggaca cattgcgacg
      781 tttcgaccat tttataacgg gtaaaaccac catcaacatg agggaaatac atggcactgc
      841 caaaaaaacg catatctgta cactgattct cgttatgttc aatgcagtat ttgcagtgac
      901 cgcacggttt agacggatta atggctaccg tttgcccttc atgtaattct gatgagtcgc
      961 tatgaataac tttaccgata acttcatgac ctaacaccat cggtgccttt atcatgaaat
     1021 tacctacttt tccttcctga taataatgta aatcggaacc gcaaattcca cctcgggtta
     1081 tttgtactaa tgttccatta ttattccaat ctatcgtctg ctcggtaacg gcaacagttt
     1141 tcttgtccgc aacaacgcag gactgtgttt tcacttgcat aaattcctca ctggtcaggt
     1201 agacacctcg gaagcattta agcggtttta attgtcatta tttgtgatga agatcacgtc
     1261 agaaaattgt tacattacta tgttacgcat aacgtgatgt gccttgtaat tcttatcagc
     1321 agaaataaaa aaacgtgaaa ttattatgcc accaggcgta gtatcgcagc aggtaagatg
     1381 attcaggaga ttttaaatgg cgggtgaaag ctttattttg atgggcgttt cagggagtgg
     1441 taaaacatta attggtagca aggttgccgc gttattatct gctaaattta ttgatggtga
     1501 cgatcttcat ccagccaaaa atatagataa aatgtcgcag ggtattccat tatctgatga
     1561 agatcgactt ccctggctgg aacgcttaaa tgatgcttca tacagtcttt ataaaaagaa
     1621 tgaaacagga tttattgtct gttcatcatt aaaaaaacag tatcgtgata ttttacgcaa
     1681 gggtagcccc catgttcatt tcctctggtt agatggcgac tatgaaacta ttctcgcgcg
     1741 aatgcagcgg cgggctgggc attttatgcc ggtagcgtta ctaaaaagtc agtttgaggc
     1801 gctggagcgt ccacaagcag atgaacagga tattgtacgc attgatatca accatgatat
     1861 tgccaatgtc accgaacaat gtcggcaggc cgtgctggcg atacgacaag acagaatatg
     1921 tgcgaaaggg ggtagcgcct cagatcagcg ctgcgaatga ttttcaaaaa tcggctttca
     1981 gaaccacgcg gtaacgcgcc ttaccgttgc gcacatgctg gatggcgtcg tttattttcg
     2041 acatcgggaa cagttcggtg gtcggcgcaa ccttgctgcg ggccgcaaag cgcatcagct
     2101 tacgaagctc ataaggtgtg ccggttgctg aaccagagac gctgcggtcg cccgcaatta
     2161 acgtaaaggc cggaacagac agcggcgtga ggaccgcacc gacggtgtgg aaattaccgc
     2221 catacgtcag cgcctcaaaa taaggctgcc agtcgaggct gacgttgacg gtgttgataa
     2281 tgagatcgaa ctgccccgca agtgttttca gtgcctgcgg atcgcggcta ttcaccactt
     2341 tatcggcacc catcgccagt acttcctgct ctttcgcagg attcgaacta aaggctgtca
     2401 cctcgcatcc cattgcgtgc agaagtttta tagcgatatg ccccagcccg ccaataccaa
     2461 ttaccccaac gcggctggta gcagtgatat ggtgcatcaa cagtggttta aagaccgtga
     2521 taccaccaca cagcagcggc ccggcggact cgatatcgat attttccggc agtgggatca
     2581 cccattgcca gtctgcacgc aacttctcgg caaaaccgcc gcgattcata attgttggca
     2641 ccgcaccttg ctcgcagttg atctgattac cgctaataca ggcgtcgcaa tgcccgcagc
     2701 tacgcgccgt ccagccaatc ccgacacgct gaccgacctg caaaccttta tcctgcgcgg
     2761 cgctcccgag cgccaccacg cgcccaatca cctcatgccc ggcaaccagc ggatactgtg
     2821 aaaatcccca ttcgttgtcg atcatcgaca gatcggaatg gcagatcccg cagtaatcca
     2881 cctgcacttc aacatcttgt ggcttcaatt caccgggctc gtactcataa acttccagtt
     2941 cgccgcccgc ttcttttgcg gcatagcttt ttatcatcga cattttttgg tccttctctg
     3001 gtgttgtctg ggaagtgtag agcatggcag ggcggggtgc ctggagtgtg acaaaggtta
     3061 cacatcgctg tatgcaatgc tgaaaatttc agcacttagc gaggtgcgag caagctggcg
     3121 cttgcatggt ggcgtgcgac aggtataatc cacaacgttt tccgcatacc tcttcagtgc
     3181 cgaagtggcg aaatcggtag acgcagttga ttcaaaatca accgtagaaa tacgtgccgg
     3241 ttcgagtccg gccttcggca ccaaaagtat gtcaatagac ctcaactgag gtctttttta
     3301 tgcctgaaat tcagtattta tctacctttc ccgctatatt aactctctca aggtcaaccg
     3361 atatcaacgt acttctacca acatatgttg gtacagatga tggtatttcc ggttcgataa
     3421 tgcttgtacc aacagggagg gaatacgcat ggcattaaca gacatcaaag tcagaacagc
     3481 taagccaaca gataagcaat ataagttgac tgatggtggc ggtatgcatc tgcttgtcca
     3541 tccaaatggt tctaagtact ggcgtttgca gtaccgttat gagggaaagc aaaagatgct
     3601 ggcacttggg gtttatcctg aaatcacact cgcggatgcc agaatacgtc gtgacgagcc
     3661 acgtaagctg cttgcgaatg gcgtcgatcc gggagacaaa aagaaaagtg ataaggttga
     3721 acagagtaaa gcacgaacct ttaaagaagt cgcgattgag tggcatggca ccaataaaaa
     3781 gtggtctgaa gatcatgccc atcgtgtgct aaaaagtctg gaagataatc tttttgcagc
     3841 gcttggtgaa cgtaatatcg ctgagttaaa aactcgagat ttattagcac ctattaaggc
     3901 cgtagaaatg tctggacgtc ttgaagtggc tgctcgtctt caacagcgc
//
LOCUS       DAKISA010000220         3947 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.223, whole genome shotgun sequence.
ACCESSION   DAKISA010000220 DAKISA010000000
VERSION     DAKISA010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.223"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            32..970
                     /locus_tag="OVW45_004353"
     CDS             32..970
                     /locus_tag="OVW45_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HCV5528632.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDNPPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            1026..2315
                     /locus_tag="OVW45_004354"
                     /pseudo
     CDS             1026..2315
                     /locus_tag="OVW45_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            2312..2605
                     /locus_tag="OVW45_004355"
     CDS             2312..2605
                     /locus_tag="OVW45_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCV5528633.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            2662..3947
                     /gene="fadK"
                     /locus_tag="OVW45_004356"
                     /pseudo
     CDS             2662..3947
                     /gene="fadK"
                     /locus_tag="OVW45_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
BASE COUNT          929 a         1004 c         1089 g          925 t
ORIGIN      
        1 gccctgaact gaagcctacg gagaagaaac gatgagtcaa ttaaacagcg tctgggtctt
       61 tagcgataat cctgaacgtt atgctgaact gtttggcggc gcgcagcaat ggggccaaca
      121 ggtgtatgcc attgtacaaa ataccgacca ggcgcaggca gttatgcctt atggtccaaa
      181 atgtatttat gttcttgagc aaaatgatgc gctgcaacgt actgaaaatt acgccgaaag
      241 cattgctgcc ctgctgaaag ataacccccc cgctatgttg ctgctggccg cgacgaaacg
      301 tggtaaagcg ctggcagcac ggttaagtgt gcaactgaat gcggcgctgg tgaacgatgc
      361 cacggcagtg gatattgtcg atggtcacat ttgcgccgaa caccggatgt atggcgggtt
      421 agcgttcgct caggaaaaga tcaacagccc gctggcgatc attacccttg cacccggtgt
      481 tcaggaaccg tgcaccagtg atacctctca tcagtgcccg acagaaacgg taccttatgt
      541 tgccccgcgt catgaaattc tctgtcgcga acgccgtgcg aaagcggcaa gcagcgtgga
      601 cctgagcaaa gcaaaacgtg tggttggcgt cggtcgtggt ctggcagcgc aggatgacct
      661 gaaaatggtc cacgaactgg cggcggtgct gaatgctgaa gtcggctgtt cacgtccaat
      721 tgccgaaggc gagaactgga tggagcgtga acgttatatc ggtgtctccg gcgtgttgct
      781 gaaatccgat ctctacctga cgctggggat ctccgggcag atccagcata tggttggcgg
      841 caacggcgca aaagtgattg tcgccatcaa taaagataaa aatgcgccaa tcttcaatta
      901 tgccgactac ggtctggtgg gcgatatcta caaagtcgtc cctgccctga ttagccagtt
      961 gagccgctaa ttcccctttc aaccacatct cccgctgtaa acgcagcggg aagggagcat
     1021 aacgcatgtc ggatgacaaa tttgatgcca ttgtggtcgg tgcgggcgtt gctggtagcg
     1081 ttgccgcact ggtcatggcg cgagccgggc tggatgtcct ggtgatagaa cgcggcgaca
     1141 gtgccggatg taaaaacatg accggcgggc gtctttatgc ccacacactt gaagcaatca
     1201 ttccaggctt tgcagtatca gcgccggtag aacgcaaggt cacacgcgag aaaatctcct
     1261 tcttaaccga agaaagcgcc gttaccctcg attttcaccg cgagcagcca gatgttccgc
     1321 aacacgcatc ttataccgta ttgcgtaatc gtctggaccc gtggttgatg gaacaagccg
     1381 agcaggcggg cgcgcagttt atcccaggcg ttcgcgtcga tgcgctggtt cgtgaaggaa
     1441 acaaggtaac tggcgtgcag gctggggatg atattctcga agcgaatgtg gtgattctgg
     1501 ctgatggcgt taactcgatg cttggccgct cgctgggaat ggttcccgct tccgatccgc
     1561 atcattacgc tgttggtgtt aaagaggtta ttggcctcac accagaacag atcaacgatc
     1621 gctttaatat tacgggcgag gaaggtgccg cctagctgtt tgccggttcc ccttctgacg
     1681 gcctgatggg tggcggattc ctctatacca acaacgattc cgtatcgttg gggctggttt
     1741 gtggattggg tgatatcgcc catgcgcaaa aaagcgtgcc gcaaatgctg gaagatttta
     1801 aacaacaccc cgccattcgc ccactaatta gcggcggcaa actgcttgaa tattccgcgc
     1861 atatggtgcc ggaaggcggt ctggcgatgg tgccgcaact ggttaacgag ggcgtgatga
     1921 tcgttggtga cgccgcaggc ttctgtctga atttgggttt tacggtccgc ggcatggatt
     1981 tagccattgc atcggctcag gctgccgcca caacggtgat cgccgccaaa gaacgcgagg
     2041 atttctccgc cagcagtctg gcgcaataca agcgtgagct ggaacaaagc tgcgtcatgc
     2101 gtgatatgca gcattttcgc aagatcccgg cgctgatgga aaacccgcgc ctgtttagcc
     2161 aatacccacg aatggtagcc gacatcatga acgagatgtt caccatcgac ggcaaaccaa
     2221 accagccggt acgaaaaatg atcatgggac atgcgaagaa aattgggctg atcaacttgc
     2281 tgaaagatgg cattaaggga gcaaccgcgc tatgagccag aacgctacgg ttaacgtcga
     2341 catcaaatta ggcgtcaata aattccatgt tgatgagggc cacccgcata tcattctggc
     2401 ggcaaatccc gacatcaatg aattccgtaa attaatgaaa gcctgccctg ccggacttta
     2461 taagcaggat gacgccggca acattcattt tgattctgcc ggttgtctgg agtgcggcac
     2521 ctgtcgggtg ctgtgcggta acactattct cgaacagtgg caatatcccg caggcacctt
     2581 cggtattgat tttcgctacg gctaatcatg catcccacag gcccgcatct cgggcctgat
     2641 gttctgtttc gagagtccaa catgaaagtg acattaacgt tcaacgaaca acgtcgtgcg
     2701 gcgtatcgtc agcaagggct atggggcgat gcttcgctgg ccgattactg gcagcagacc
     2761 gctcgtgcga tgccagacaa aattgccgtg gtcgataatc atggtgcatc gtacacctat
     2821 agcgcgctcg atcacgccgc gagctgtctg gcaaactgga tgttagcgaa gggtattgaa
     2881 tcaggcgatc gcatcgcatt tcaactgcca ggctggtgtg aatttaccgt tatctatctt
     2941 gcctgcctga aaaccggtgc ggtttccgtg ccgctgttgc cttcctggcg ggaagcagaa
     3001 ctggtgtggg tgctcaataa gtgtcaggca aaaatattct ttgcaccgac gttgtttaaa
     3061 caaacgcgtc cggtagattt aatcctgccg ctgcaaaatc agcttccaca actacaacaa
     3121 attgtcggcg tggacaaact ggctcccgcc acctcttccc tctcattaag tcagattatc
     3181 gccgacaata tcccactgac cacggcgata acgacccacg gcgatgaatt agctgcggtg
     3241 ctgtttacct ccggaaccga gggtctgcca aagggcgtaa tgctaacgca taacaatatt
     3301 ctcgccagtg agcgggctta ttgcgcgcgg ctgaatctga cctggcagga tgtctttatg
     3361 atgcctgcgc cacttggtca cgcaacggga tttctgcatg gcgtaacagc accattttta
     3421 attggtgctc gcagcgtgtt gttagatatt ttcactcctg atgcatgtct cgcgctgctt
     3481 gagcagcagc gttgcacctg tatgctcggc gcaacgccgt ttgtctatga tcttttgaat
     3541 ttactagaga aacaacccgc agacctttca gcgctgcgct tctttctttg cggcggtacc
     3601 acaatcccca aaaaagtggc gcgcgaatgc cagcagcgct ggcattaaat tattaagtgt
     3661 ttatggttcc acagaaagtt cgccgcatgc ggtggtgaat ctcgatgatc ctttgtcgcg
     3721 ctttatgcac accgatggtt acgctgccgc aggtgtagag attaaagtgg tcgatgacgc
     3781 acgcaagacc ttaccgccag gttgcgaagg tgaagaagcc tcgcgtggcc ccaatgtgtt
     3841 tatggggtat tttgatgaac ctgaattaac cgcccgtgcg ctggatgaag aaggctggta
     3901 ttacagcggc gatctctgcc gtatggatga ggctggctat ataaaaa
//
LOCUS       DAKISA010000221         3909 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.224, whole genome shotgun sequence.
ACCESSION   DAKISA010000221 DAKISA010000000
VERSION     DAKISA010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.224"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            25..591
                     /locus_tag="OVW45_004357"
     CDS             25..591
                     /locus_tag="OVW45_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCV5528634.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            595..1170
                     /locus_tag="OVW45_004358"
     CDS             595..1170
                     /locus_tag="OVW45_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCV5528635.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQQ"
     gene            complement(1212..2264)
                     /locus_tag="OVW45_004359"
     CDS             complement(1212..2264)
                     /locus_tag="OVW45_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCV5528636.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2489..3409
                     /gene="lpxL"
                     /locus_tag="OVW45_004360"
     CDS             2489..3409
                     /gene="lpxL"
                     /locus_tag="OVW45_004360"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCV5528637.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3581..3778
                     /locus_tag="OVW45_004361"
     CDS             3581..3778
                     /locus_tag="OVW45_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCV5528638.1"
                     /translation="MSPCGNDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIIPS"
BASE COUNT          969 a          989 c          953 g          998 t
ORIGIN      
        1 cgcaaattcg tattggatgc acttatgtca ttcacaaata cccctgaacg ctatggtgtt
       61 atatctgcgg ccttccattg gctaagcgca attattgtct acggcatgtt tgctttaggt
      121 ctgtggatgg tcacgctcag ttattacgat ggctggtatc acaaagcacc cgaactgcat
      181 aaaagcatcg gtattttgtt aatgatgggg ctggttattc gcgttctgtg gcgtgtcata
      241 tctccaccgc cgggtccgct gccgagttat tcgcccatga ctcgtcttgc cgcaaaggct
      301 ggacatctcg cattgtacct attgctgttc gccatcggca tcagcggcta tctgatctca
      361 actgccgatg gtaaaccgat cagcgttttt ggctggtttg acgtccccgc gacccttgcc
      421 gacgctggcg cacaggcaga ctttgccggt gccctgcatt tttggctcgc gtggagcgtc
      481 gtagtactgt ccgttatgca cggatttatg gccctgaagc atcatttcat cgataaagac
      541 gacactctga agcgcatgct gggaaagtcg tcatctgact atggagtata aaaaatgaaa
      601 aaaagcctgc ttggtttaac cttcgcgtcc ctgatgttct ctgccggttc agcggttgcc
      661 gccgattaca aaattgacaa agaaggtcag cacgcctttg ttaatttccg catccagcac
      721 cttggctata gctggttata cggcaccttt aaagatttcg acggtacttt tacctttgac
      781 gaaaaaaatc cggctgccga taaagtgaat gtgacaatta acaccaccag cgtcgatact
      841 aatcacgccg aacgcgataa acatcttcgc agtgcagatt tcctcaatac cgcaaaatat
      901 ccacaggcaa cattcacctc caccagcgtg aagaaagacg gtgacgaact ggatattacc
      961 ggcgatctga cgctgaatgg cgtaaccaaa cccgtcacgc tggaagcgaa attaattggt
     1021 cagggcgacg acccatgggg tggtaaacgt gcaggcttcg aggccgaagg caaaattaag
     1081 ctcaaggact tcaatatcaa gacagattta ggtccagctt ctcaggaagt ggatctgatt
     1141 atttcagtgg aaggcgtaca gcagcagtaa gcgtgaaatg ttgccggatg gcacgcatcc
     1201 ggcaatgata tttattctgt tggatcagga atgcccagtg ttgtattcag acgtccacga
     1261 gacttattaa agatcttatt gccattttca cgtcctgccc gacggcgtcg ctgttcttct
     1321 ggcggtaacg cgctttcttc gcagcaaatc tcactacaac aacctttgta tttttccgcg
     1381 catactggac actgaataaa cagcaggtgg cagccatcat ttttacagtt ggtatggctg
     1441 tcgcacggcg cgccgcactg gtggcaatgc gcgataatct catccgatat acgttcgccc
     1501 atccgctcgt caaaaacaaa atttttgcca ataaaacgca ccggcaagcc ctgctcgcgc
     1561 gccttacggg cgtattcaat aattccgccc tcgatatgcc agactttgtt gaatccgtta
     1621 tgtttcatcc aggcactggc cttttcacaa cgaatgccgc cggtgcagta catgacgatt
     1681 tttttatctt tatgtgcctg catcatctcg actgcttttg gcagctgctc acggaaggta
     1741 tctgccggaa tttcgagcgc gttttcaaag tgccccactt catactcata gtggttacgc
     1801 atgtcgataa atagtgcatc gggatcgtca agcatggcgt tcacttccgc cgcttgcaga
     1861 tactcaccaa cattgctggc atcaaagtga ggatcgtcaa taccgtcggc aacaatgcga
     1921 tcgcgtacct tcatgcgcag tacccagaag gatttcccgt catcatccaa cgcgatattc
     1981 aggcgtaaac cctctaaagc cgggtcgaag gcatagagct gcgcgcgaaa tgtttcaaca
     2041 ttgctcgcag gtacgctgat ttgcgcgtta atgccctcat gcgccagata cactcgccca
     2101 aaaacattca gcgcggtaaa cagctgatat aaagcgtcac gggtcgcctt aggatcggcg
     2161 atgtggaaat acttgtaaaa cgaaatggtg gttcgcggtt cgctctcagc caacattttg
     2221 gcttttaacg cgtcgttgga aatgcggttg tgtaacactg gcatggtgta cggttcctgc
     2281 gagatgggaa agtaaaaatc cgcggcatga tatagcaatt atcgataatt aacatccaca
     2341 cattttacgc tacatttgcg cattaaaaat tatttgttat ttacaagcgc ggcaatttcg
     2401 cccagtcttc agccacaaat tttggttgcg ggcgaaaaaa tgcgacaata catacaattg
     2461 cccgaatagg ttgaaaaaca ggattgatat gacgaatcta cccaagttct ccaccgcact
     2521 gcttcatccg cgttattggt taacctggtt gggtattggc gtactttggt tagtcgtgca
     2581 attgccctac ccggttatct accgcctcgg ttgtggatta ggaaaactgg cgttacgttt
     2641 tatgaaacga cgcgcaaaaa ttgtgcatcg caacctggaa ctgtgcttcc cggaaatgag
     2701 cgaacaagaa cgccgtaaaa tggtggtgaa gaatttcgaa tccgttggca tgggactgat
     2761 ggaaaccggt atggcgtggt tctggccgga ccgccgaatc gcccgctgga cggaagtgat
     2821 cggcatggaa cacattcgtg acgtgcaggc gcaaaaacgc ggcatcctgt tagttggcat
     2881 ccattttctg acactggaac tgggcgcgcg gcagtttggt atgcaggaac cggggattgg
     2941 cgtttaccgc ccgaacgata atccactgat tgactggcta caaacctggg gccgtttgcg
     3001 ctcaaataaa tcgatgctcg accgcaaaga tttaaaaggc atgattaaag ccctgaaaaa
     3061 aggcgaagtg gtctggtacg caccggatca tgattacggc ccgcgctcaa gcgttttcgt
     3121 cccgttattt gccgttgagc aggctgcgac cacgaccgga acctggatgc tggcacggat
     3181 gtccggcgca tgtctggtgc ccttcgttcc acgccgtaag ccagatggca aagggtatca
     3241 attgattatg ctgccgccag agtgttctcc gccactggat gatgccgaaa ctaccgccgc
     3301 gtggatgaac aaagtggtcg aaaaatgcat catgatggca ccagagcagt atatgtggtt
     3361 acaccgtcgc tttaaaacac gcccggaagg cgttccttca cgctattaaa tctcccctgc
     3421 cggatgcttc agaatggcat ccggcattac cacagcaaat ccccctgatt tagcgataaa
     3481 agctctctgg attgcgcccc ctggaagtcg ggcgcataat tagtgtgctt atcttttctt
     3541 cttatgttca ccgcgcctgg cgcaccaaca gcggattgct atgtcaccct gtggaaatga
     3601 cacccctata aactggaaac gaaacctgat cgtcgcctgg ctaggctgtt ttcttaccgg
     3661 tgccgccttc agtctggtaa tgcccttctt acccctctac gttgagcagc ttggcgttac
     3721 cggtcactcc gccctgaata tgtggtccgg tattgtcttc agtataatac cttcctaaat
     3781 acactacctg agagaagaga tgaaaagagc tatcagctac atgaggtttt caactgagaa
     3841 gcagataaaa ggggactcta tacgaagaca gtctaagctg gtcactgact ggttatcaaa
     3901 gaaccctga
//
LOCUS       DAKISA010000222         3827 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.225, whole genome shotgun sequence.
ACCESSION   DAKISA010000222 DAKISA010000000
VERSION     DAKISA010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.225"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            247..768
                     /gene="fecI"
                     /locus_tag="OVW45_004362"
     CDS             247..768
                     /gene="fecI"
                     /locus_tag="OVW45_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HCV5528639.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            765..1718
                     /gene="fecR"
                     /locus_tag="OVW45_004363"
                     /pseudo
     CDS             765..1718
                     /gene="fecR"
                     /locus_tag="OVW45_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
     gene            1805..>3827
                     /gene="fecA"
                     /locus_tag="OVW45_004364"
     CDS             1805..>3827
                     /gene="fecA"
                     /locus_tag="OVW45_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HCV5528640.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVD"
BASE COUNT          898 a         1130 c         1035 g          764 t
ORIGIN      
        1 cagcattacc gaaatcccta tcctttaggg cagtgaggat gtcaataata gcagaccaca
       61 tcatgagtca ttctgaccga cacatgccag aagcagaggc tccatcccat tttataccta
      121 ccttataaca cttagaaaaa caacatgtta aaaatgtcta ttggaaacaa ttttatttcc
      181 aattgtaatg ataactattc tcatattaat atggctacgt gataattaac ttttgatgca
      241 ctccgcatgt ctgaccgcgc cactaccaca gcttccttaa cattcgagtc gctttatggc
      301 acacatcacg gctggttgaa aagctggctg acgcgcaaac tccagtctgc ttttgatgca
      361 gatgacattg cccaggacac ttttttgcgg gtaatggtca gcgaaacgct ctcgacgatc
      421 cgcgatcctc gctccttcct ctgcactatc gccaaacgcg tgatggtgga cctgtttcgc
      481 cgaaacgcgc tggaaaaagc gtatctggag atgctggcgc ttatgccgga ggggggagcg
      541 ccttcacctg aggaacgcga aagccaactc gagaccctac aactcctcga cagcatgctg
      601 gacgggctaa acggcaaaac acgtgaagcg tttctgcttt cgcaactgga tggtctgaca
      661 tacagcgaga ttgcgcacaa actcggtgtt tccatcagct ccgtgaaaaa atacgtggcg
      721 aaagccgtcg agcactgcct gctgttccgt ctggagtatg ggttatgaat cctttgttaa
      781 ccgattcccg ccgtcaggcg ctgcgttcag cttcccactg gtatgccgtg ctaagcggcg
      841 agcgcgtcag cccacaacag gaagcgcgct ggcaacagtg gtatgaacag gatcaggata
      901 accagtgggc ctagcagcag gttgaaaacc tgcgcaacca gcttggcggt gtgcctggcg
      961 acgttgccag ccgggcgttg cacgataccc gcctcacccg ccgtcacgtg atgaaaggat
     1021 tactgctgtt gctcggcgct ggcggaggct ggcagctctg gcagtcggaa accggcgaag
     1081 gtctgcgggc agattaccgc accgccaaag gcacagtcag ccgtcagcaa ctggaagatg
     1141 gctccctgct cacgctgaat acccaaagcg cggcggatat gcgttttgat gcgcatcagc
     1201 gcaccgtccg gctctggtac ggtgaaatcg ccattactac cgcgaaagat gccctgcaac
     1261 gccccttccg tgtcctgacc cgtcagggcc agctcactgc tttagggaca gaatttaccg
     1321 tccgccagca ggataatttc acgcagcttg acgtgcagca gcacgccgtg gaagtgcttc
     1381 tcgccagtgc ccccgcgcaa aaacgcatcg tgaacgctgg tgaaagcctg cagttcagcg
     1441 cctctgagtt tggcgcagtg aaaccgctgg atgacgagag tacaagctgg acgaaggaca
     1501 tcctgagctt cagcgataaa ccgctgggtg aggtgatagc cacgctaacc cgttaccgca
     1561 acggcgtgct gcgctgcgat cccgccgttg ccgggctgcg cctgagcggg acgttcccgc
     1621 tgaaaaatac cgatgcgatc ctgaacgtta tcgcgcaaac gcttcccgtt aaaattcagt
     1681 ctattacgcg gtactggata aacatttcac cactgtaagg aaaataattc ttatttcgat
     1741 tgtccttttt acccttctcg ttcgactcat agctgaacac aacaaaaatg atgatgggga
     1801 aggtatgacg ccgttacgcg tttttcgtaa aacaacacct ttggttaacg ccattcgcct
     1861 gagcctgctg ccgctggccg gtctctcgtt ttccgctttt gctgcacagg ttaatatcgc
     1921 accgggatcg ctcgataaag cgctcaatca gtatgccgca cacagcggat ttaccctctc
     1981 ggttgacgcc agcctgacgc gcggcaagca gagcaacggc ctgcacggcg attacgacgt
     2041 cgagagcggc ctgcaacaac tgctggacgg cagcggactg caggtaaaac cgctgggaaa
     2101 taacagctgg acgctggagc ccgcgcccgc accaaaagaa gatgccctga ccgtggtcgg
     2161 cgactggctg ggtgatgcgc gtgaaaacga cgtatttgaa catgctggcg cgcgtgacgt
     2221 gatccgccgt gaggatttcg ccaaaaccgg cgcaaccacc atgcgtgagg tacttaaccg
     2281 catccctggc gtcagcgcgc cggaaaacaa cggcaccggc agccacgacc tggcgatgaa
     2341 ctttggcatc cggggcctga acccgcgcct cgccagccgc tcgaccgtcc tgatggacgg
     2401 catccccgtc cccttcgccc cttacggtca gccgcagctt tcactggctc ccgtttcgct
     2461 cggcaacatg gatgccattg acgtggtacg cggtggtggt gcggtgcgtt acggaccgca
     2521 gagcgtgggc ggcgtggtga actttgttac ccgtgccatt ccgcaggact ttggtatcga
     2581 ggcgggcgtg gaaggtcagc tcagcccaac ctcttcacaa aacaacccga aagagacgca
     2641 caacctgatg gtgggcggca cagcggacaa cggttttggc accgcgctgc tctactccgg
     2701 cacgcgcggc agtgactggc gcgagcacag cgccacccgc atcgacgacc tgatgctgaa
     2761 aagcaaatat gcgccggatg aggtgcacac cttcaacagc ctgctgcaat attacgacgg
     2821 tgaagccgac atgcccggtg gcctgtctcg cgcggattac gacgccgatc gctggcaatc
     2881 cacccgcccg tatgaccgct tctggggtcg tcgcaagctg gcgagcctgg gctaccagtt
     2941 ccagccagac agccagcata aattcaacat tcaggggttc tacacccaaa ccctgcgcag
     3001 cggctacctg gagcaaggca aacgcatcac cctctcgccg cgtaactact gggtgcgcgg
     3061 tattgagcca cgctacagcc agatctttat gatcggccct tccgcgcacg aagtgggcgt
     3121 gggctatcgc tatgtgaatg aatcaacgca tgaaatgcgt tactacaccg ccaccagcag
     3181 cgggcagttg ccgtccggct caagccctta cgaccgcgat acgcgttccg gcaccgaggc
     3241 gcacgcctgg tatctggatg acaaaatcga catcggcaac tggaccatca cgccgggtat
     3301 gcgtttcgaa catatcgagt cataccagaa caacgccatc acaggcacgc acgaagaagt
     3361 gagctataac gcaccgcttc cggcgttgaa cgtgctctat cacctgactg acagctggaa
     3421 tctttatgca aacactgaag gctcgttcgg caccgtacag tacagccaga ttggcaaggc
     3481 tgtgcaaagc ggcaatgtgg aaccggaaaa agcgcgaacc tgggaactcg gtacccgcta
     3541 cgacgacggc gcgctgacgg cggaaatggg gctgttcctg attaacttta acaatcagta
     3601 cgactccaac cagaccaacg acaccgtcac tgcacgtggc aaaacgcgcc ataccgggct
     3661 ggaaacgcag gcacgttacg atctgggtac gctaacgcca acgcttgata acgtttccat
     3721 ctacgccagc tatgcgtatg tgaacgcgga aatccgcgag aaaggcgaca cctacggcaa
     3781 tctggtacca ttctccccga aacataaagg cacgctgggc gtggact
//
LOCUS       DAKISA010000223         3797 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.226, whole genome shotgun sequence.
ACCESSION   DAKISA010000223 DAKISA010000000
VERSION     DAKISA010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.226"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(98..907)
                     /gene="proC"
                     /locus_tag="OVW45_004365"
     CDS             complement(98..907)
                     /gene="proC"
                     /locus_tag="OVW45_004365"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCV5528641.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            1027..1485
                     /locus_tag="OVW45_004366"
     CDS             1027..1485
                     /locus_tag="OVW45_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCV5528642.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            1668..2192
                     /gene="aroL"
                     /locus_tag="OVW45_004367"
     CDS             1668..2192
                     /gene="aroL"
                     /locus_tag="OVW45_004367"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCV5528643.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            2242..2433
                     /gene="yaiA"
                     /locus_tag="OVW45_004368"
     CDS             2242..2433
                     /gene="yaiA"
                     /locus_tag="OVW45_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCV5528644.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            2691..3368
                     /locus_tag="OVW45_004369"
     CDS             2691..3368
                     /locus_tag="OVW45_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCV5528645.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            3440..3724
                     /gene="ppnP"
                     /locus_tag="OVW45_004370"
     CDS             3440..3724
                     /gene="ppnP"
                     /locus_tag="OVW45_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCV5528646.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT          951 a          902 c          990 g          954 t
ORIGIN      
        1 cgtgagtggt tgaaatcggc agatttggcg ctttacaaag caaagaaagc cggacgtaac
       61 cgcaccgaag tggcggcctg acgtccggcg aaagtcatca ggatttgctg agtttttctg
      121 atttttccat acacttcgtc atcgcttcga tcactgcggc acggaagcct ttctcttcca
      181 gtacgcgtac cgcttcaatg gtggtgcctc ccggtgagca gaccatatct ttcagtgccc
      241 ccggatgttc tcccgtttcc agcaccattt ttgcggaacc cattaccgcc tgagcggcaa
      301 atttatacgc ctgggcgcgt ggcatcccgc ccagcacggc ggcgtcggcc atcgcttcga
      361 taaacataaa tacgtaggct ggcgaagaac cgctcacacc gaccaccggg tggatcatcg
      421 gctcagcaat tacttccgct tcgccaaagc agcggaaaat attcagcaca tcagcggtat
      481 cttcgggggt taccagcgcg tttggcgtta cggaggtcat cccggcatta accagcgcgg
      541 gagtgttcgg catggcgcgg ataattttcc ggtcatggcc cagcgcgcgg gcaagctggt
      601 cgagcgtgac acctgcagca atagaaacga ccagagagtc tttattcagg ctggaggtga
      661 tttcgctaag cactttaatc atgatgccag gtttaacggc ggcaaaaatg atgtcggcga
      721 tttgcgccac ttcttgcgcc gattctgcgg cgttgatgcc gaactggtca tgcagggcgg
      781 cgactttatc cggggagggg gtgtataccc agatttgccc tggaagcacc tgaccgctgg
      841 caatcagacc gccgagaatg gcttttccca tattgccgca gccaataaaa ccgattttct
      901 tttccattgc ctcactcctg ccgtgaaatt cattgttttg ataatcgctg gcagaagcat
      961 aaacagaact atgccggaag gcaaaagcgc gacacaatag aggattaccc aacaaaggat
     1021 gactttatga caatttgggt ggatgccgac gcgtgtccca atgtaattaa agagattttg
     1081 tatcgcgcgg cggaacgtat gcagatgccg ctggtactgg tagcaaacca gagtttacgt
     1141 gtgccgccat cgcgatttat tcgtacgctg cgcgtcgcgg caggtttcga cgttgccgat
     1201 aacgaaattg tccggcagtg tgaagcgggc gatttggtga tcaccgcaga tatacctttg
     1261 gctgctgaag ccatcgagaa aggcgcagcg gcgcttaatc cgcgcggcga acgttacacg
     1321 ccagcgacca ttcgtgagcg cctgacgatg cgcgatttta tggatacctt acgtgccagt
     1381 gggatccaga ccggcggacc agatagcctt tcacaacgtg accgccaggc ctttgccgcg
     1441 gagctggaga agtggtggct ggaagtgcaa cgtagtcgtg gctaaatgta atttattatt
     1501 tacacttcat tcttgaatat ttattggtat agtaagggat gtattgagat tttcacttta
     1561 agtggaattt tttctttaca atcgaaattg tactagtttg atggtatgat cgctattctc
     1621 atgacaccgg ctttcgccgc attgcgacct attggggaaa acccacgatg acacaacctc
     1681 tttttctgat cgggcctcgg ggctgtggta aaacaacggt cggaatggcc cttgccgatt
     1741 cgcttaaccg tcggtttgtc gataccgatc agtggttgca atcacagctc aatatgacgg
     1801 tcgcggagat cgtcgaaagg gaagagtggg cgggatttcg cgccagagaa acggcggcgc
     1861 tggaagcggt aactgcgcca tccaccgtta tcgctacagg cggcggcatt attctgacgg
     1921 aatttaatcg tcacttcatg caaaataacg ggatcgtggt ttatttgtgt gcgccagtat
     1981 cagtcctggt taaccgactg caagctgcac cggaagaaga tttacggcca accttaacgg
     2041 gaaaaccgct gagcgaagaa gttcaggaag tgctggaaga acgcgatgcg ctatatcgcg
     2101 aagttgcgca tattatcatc gacgcaacaa acgaacccag ccaggtgatt tctgaaattc
     2161 gtagcgccct ggcacagacg atcaattgtt gattttagag cgcctatact taacgttcat
     2221 cccgtgaaat aaggaagaac gatgccaacg aaaccgcctt atcctcgtga agcatatata
     2281 gtgacgattg aaaaaggaaa gccaggacag acggtaacct ggtaccaact cagagccgat
     2341 catcctaaac cagactcgtt gatcagtgaa catccgacgg ctcaggaagc gatggatgcg
     2401 aaaaaacgct atgaggaccc tgacaaagag tgaccgcatc agactgctcg gaagggattc
     2461 tgagtgccac tacaagggat ctgcgtcaca tttttcataa ttcatgtttt tctaataatt
     2521 agaatattaa acaataacaa tccattactg gaatcatttg gaatctttac attatgccgt
     2581 gcacgtctgc tgctacgctt tttgtcattt gtagcacaag taagtgtcag cagtggtgct
     2641 tcacacttgc ccggtaatta acgacgaaag aaaagtaagg tggatgaaca atgagtgcgt
     2701 cgttggcgat cctgaccatc ggcattgtac ctatgcagga ggttttgccg ctcctgacgg
     2761 aatacattga cgaagataat atttcccatc atagcctgct ggggaagtta agtcgtgaag
     2821 aagtgatggc ggagtacgcg ccagaagcag gcgaagacac cattctcaca ttattaaatg
     2881 acaaccagct ggcccatgtt tcgcgtcgta aagtggagcg agacctgcaa ggtgtggttg
     2941 aagtgctcga taatcagggt tatgacgtca ttttattaat gagtacagca aacattagta
     3001 gtatgactgc gcgtaatacg atctttcttg agccgtcgcg aatattgcct ccactggttt
     3061 cctctattgt tgaagatcat caggtggggg ttatcgttcc ggttgaggag atgctgaccg
     3121 ttcaggcgca aaaatggcaa attttgcaga aatcgccggt attttcattg ggtaacccca
     3181 ttcatgattc agaacaaaaa atcattgatg ccgggaaaga attactggca aaaggggctg
     3241 atgtcatcat gctggattgt ttaggatttc accaacgtca tcgcgattta ctgcaaaaac
     3301 agctcgatgt tcctgtcttg ctgtctaacg tattgattgc acggctggct gcggaattac
     3361 tggtgtaatt ttgcgtgaca gccagcgcct ctggccccta tagtgaagta gatgttcaac
     3421 taccagacag ggccagttta tgcttcaaag taatgagtac ttttccggca aagtgaaatc
     3481 aatcggcttt tccagcagca gcactggtcg cgccagcgtg ggtgttatgg ttgaaggcga
     3541 atacaccttc agcaccgctg agccggaaga gatgacggta atcagtggcg cgctgaatgt
     3601 gttactgcct gacgcgaccg actggcaggt gtatgaagcc ggttcggtgt ttaatgttcc
     3661 cggtcacagt gagtttcatc tgcaagttgc cgaacccacc tcttatctgt gccgctatct
     3721 gtaattcctc gccttcccct tgaacgggag ggcatttttc tgaaatatcc tctctttaac
     3781 ccataataat atttcct
//
LOCUS       DAKISA010000224         3734 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.227, whole genome shotgun sequence.
ACCESSION   DAKISA010000224 DAKISA010000000
VERSION     DAKISA010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.227"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..181)
                     /locus_tag="OVW45_004371"
     CDS             complement(<1..181)
                     /locus_tag="OVW45_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HCV5528647.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSI"
     gene            425..682
                     /locus_tag="OVW45_004372"
     CDS             425..682
                     /locus_tag="OVW45_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HCV5528648.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(732..1682)
                     /gene="uspE"
                     /locus_tag="OVW45_004373"
     CDS             complement(732..1682)
                     /gene="uspE"
                     /locus_tag="OVW45_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCV5528649.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1834..2586)
                     /gene="fnr"
                     /locus_tag="OVW45_004374"
     CDS             complement(1834..2586)
                     /gene="fnr"
                     /locus_tag="OVW45_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCV5528650.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2781..3296)
                     /gene="ogt"
                     /locus_tag="OVW45_004375"
     CDS             complement(2781..3296)
                     /gene="ogt"
                     /locus_tag="OVW45_004375"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCV5528651.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3307..>3734)
                     /locus_tag="OVW45_004376"
     CDS             complement(3307..>3734)
                     /locus_tag="OVW45_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HCV5528652.1"
                     /translation="FIAVGLTDILESSGLSGIPAFVGLALLSSFLCMFIASGSAIWSI
                     LAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPFVPLFLGFLQRYKPDAKLGT
                     YYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS"
BASE COUNT         1002 a          886 c          893 g          953 t
ORIGIN      
        1 taatgctacc aacagcactg ataattatgg tgtagattaa aaattgcacc gcatggaata
       61 aaaaaccttt tcttttacga tttccgcggc gaaaccaaaa gctcatcaga atcaatgctg
      121 cgcagctacc gaaaataatg accagattaa gcgcattatt tgtaaacagt tcagcgatca
      181 ttgttttatc aggctcctcc agataattgt cgtcatgccg gaaacccctg gcggggctat
      241 tttaccgcga caattcattc agatcatcaa tagtcaggga aggaagtagc aacattagct
      301 aactaatcaa tgcgttgatt gtaaatccag ctaagaggtg aggttttcag agcagacaac
      361 ggtgaaatgt catggtattg ttacgtttag gtaacaagaa atttgtctgc acaaggatta
      421 catcatgatt atggcgaaac tgaagtcagc gaaagggaaa aaatttctct ttggtttgtt
      481 ggcggttttc attattgcgg cgtcggttgt gactcgcgcg actatcggcg gcgttataga
      541 acagtacaat attccgctgt ctgagtggac gacatcaatg tatgtgattc agtcatcgat
      601 gatttttgtt tatagcctgg tctttactgt gttgctggca atcccgttgg gaatttattt
      661 ccttggcggc gaagagcagt aagtaaaaaa taggcccgat aactcgggcc ttgtcagtta
      721 ttgaagagtc gttaatcgtc ttcttcgtca tccagttcaa cgggtgtctg atactggtca
      781 ggtttaatga ccaacagatc gcagcgaaga tgatcaatca cctgttccgc cgtgttgccg
      841 aggaatgctg ctgaaatacc ggtgcgtcct accgtgccca gaaccacaat ccccgcctgt
      901 aagtgctccg ccaaatcagg aataacctct tccggcagac ctttttctac gtgcgtcatg
      961 ttttcattaa tgccgaattt ctgccgcaga gctttcattg ccagcaaatg ttgcccacga
     1021 atggcatcgt tataaacgct cggatcaaat tccggcagtt caatcgcgat attaattggc
     1081 gttactgggt aagcgccaac cagatgaact tcggtatggt tgacttgttc tgccagttcg
     1141 atcgtctctt tgaccagttt ttcattgagc gcattatgat acggctcttc actggcgaga
     1201 ttcaccgcca ccagcgcctt acctccttcc ggccacggct ggtctttcac catccacacc
     1261 gggcttgggc atttgcgtaa cagatgccag tccgttggcg taaaaatcac cgcttccaga
     1321 cggtcatgtt ggtgcgccat ttttagcacc aaatcgtgcc cgccgctgat cacttcctga
     1381 atgatggctt cgaagggacg gttatgccag accactttaa tttcaatggg aacgccagca
     1441 ttgagataat attttgcctg ctcgtggatc caggctgtac gctggctgat gacgccctga
     1501 cgcatagcgg tacgttcgtc cggggagagc agggtggtca tttcgtatga gaagtcatag
     1561 atcggcaaaa aggctttaat tttgccacca atccgttgat gtaaataaac agctcgccgc
     1621 aatgctggtt ggtcgtcctg gttaggatcg ataacaacga gcatgttctg atacatagcc
     1681 atacagggtc tccttacaac aactgtcaac gcagtttgta attaaaagat taacccatat
     1741 ctggtgaatg aaacagtgat gaaccttctg ccagatcaat aaatcagaaa aatttaatga
     1801 tatgacagaa ggatagtgag ttatgcggaa aaatcaggca acattacgcg tatgaccagc
     1861 aagctgggcc agcgcatcgt tattttcgat ggtgatgtat ttacctttga ctgccagcat
     1921 accgcttttc tggaagcgac ctagcagacg gctgatggtt tctaccgtca ggcccagata
     1981 gttaccgata tcgccacgag tcatcgtcag gcggaattca cgaggggaga agccgcgttg
     2041 ggcaaaacga cgggacaggt tgtagatgaa tgcagccaga cgttcctcgg catttttctt
     2101 cgacaacagc aagatcatgt cctgatcgcc tttgatttca ccgctcatca gacgcatcat
     2161 ctgctgacgc agattcggca ttttaccgga caaatcgtcc agcgtttcga acgggatttc
     2221 acataccatc gaggtttcca gcgcctgagc gaagctcggg tgatggccgc tgccgatggc
     2281 gtcaaatccc accaggtcgc cagctaaatg gaaaccagtg atttgctcgt cgccttgctc
     2341 agtgatggta taacttttaa tcgtaccgga gcggatggca taaagcgatt taagttcatc
     2401 accagcctta aacagcgtct ggcctttctg aataggcttc ttccgctcaa tgatattatc
     2461 aagctgatca agctcatgtt cgttgagtgt gaacgggatg caaagctggc tgatgctgca
     2521 atcctggcaa tggatagcac aaccgccaga ctgaatgcgc cgtataattc gcttttccgg
     2581 gatcataggt ctgctcaagc cgtaattgat atttgtcaat tttaacatct ttttagggag
     2641 caagtaagtc taagcaaacc ttaacagcag agaattccga tattagatgt aaatatatgt
     2701 ctatctattt gaaaaccctt aagttgttaa gggtaacttt acataaaagt gtgaacaagc
     2761 tggcacaaat tgtttaatgt ttacagcaaa agataacctt cgtggcgcaa taaccactct
     2821 tttcgctgaa ctccgcctgc atatccggtc atggtgccgt ttcggccaat aacccgatgg
     2881 caaggtacga cgatgctgat gggattcgat ccgtttgccg caccaacggc acgcgccgcg
     2941 ccagggcggc ccaattgttc agccagttgg ccgtaatgca ttacctgtcc gcaggggata
     3001 gtgcgtagag ttttccagac ttcgcgctga aatggcgtcc cccctgtggc agtgggaagc
     3061 gtatcaataa tgctaagatt accggcaaaa taatcacgaa gcttgtcgct taaaccgcct
     3121 gggttggtgg cagaaatgcg ctcatagcct tctttgcgat aatggatgtc cagcagctgc
     3181 accatgcgtt cgctgtactc ttcccattca accgcccgca ggcgaaattg ctcatcgcaa
     3241 atcacccaca gtggacccag tggcgtggca attttttctt caagtaatct cagcatccgt
     3301 tctctcttaa gacaaacgtg ggtaaatccc cggacctatc ggcagaccaa caagatacca
     3361 cgccagcaac atcagcagcc ataccaccaa aaagataagc ggatagggca agactaacga
     3421 atagtaagta cccagtttcg cgtctggttt gtagcgttgc aggaatccaa gaaacagtgg
     3481 aacaaaagga gataccggcg ctaaaggcaa tacggatgag tcggcaatac gaaagaggat
     3541 ttgcgcaaat gccgggtgaa agccaagtag cataaacatt ggtacgaaaa tgggggccag
     3601 aatcgaccag attgcggaac cgctggcaat aaacatgcat aagaaagagg aaagcaacgc
     3661 cagaccgaca aacgccggga tgccgctaag ccctgaactt tccagtatat cggtcagccc
     3721 cacggcgatg aatt
//
LOCUS       DAKISA010000225         3729 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.228, whole genome shotgun sequence.
ACCESSION   DAKISA010000225 DAKISA010000000
VERSION     DAKISA010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.228"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(15..140)
                     /locus_tag="OVW45_004377"
                     /pseudo
     CDS             complement(15..140)
                     /locus_tag="OVW45_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(193..1449)
                     /locus_tag="OVW45_004378"
     CDS             complement(193..1449)
                     /locus_tag="OVW45_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCV5528653.1"
                     /translation="MDFFRFLMSDVLSEPAVLVGLIALIGLIAQKKPVTECIKGTVKT
                     IMGFVILGAGAGLVVSSLGDFANIFQHAFGIQGVVPNNEAIVSVAQKSFGKEMAMIMF
                     FAMVINILIARFTPWKFIFLTGHHTLFMSMMVAVILATAGMTGITLIAVGSLVVGVAM
                     VFFPAIAHPYMKKVTGSDDVAIGHFSTLSYVLAGFIGSKFGNKEHSTEDMNVPKSLLF
                     LRDTPVAISFTMSIIFLVTCLFAGADVVKELSDGKNWFMFSIMQSITFAAGVYIILQG
                     VRMVIAEIVPAFKGISDKLVPNARPALDCPVVFPYAPNAVLVGFLSSFAAGLIGMFSL
                     YLLNMTVIIPGVVPHFFVGAAAGVFGNATGGRRGAIWGAFAQGLLITFLPVFLLPVLG
                     DIGFANTTFSDADFGALGILLGIIVR"
     gene            complement(1462..1749)
                     /locus_tag="OVW45_004379"
     CDS             complement(1462..1749)
                     /locus_tag="OVW45_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCV5528654.1"
                     /translation="MKITVVCGNGLGTSLMMEMSIKSILKDLSVSADVDHVDLGSAKG
                     TPSDIYVGTKDIAEQLVAQSVAGKIVALENMIDKKAMRERLSVVLTELGAL"
     gene            complement(1765..2208)
                     /locus_tag="OVW45_004380"
     CDS             complement(1765..2208)
                     /locus_tag="OVW45_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCV5528655.1"
                     /translation="MLKNLLNTEVVQVVEQAKDWREAVAISCRPLIENGSIEPRYVDA
                     IYRSHDTIGPYYVVGPGIAMPHARPEEGANKLSLALTLIPSGVNFDADENDPVKLLIV
                     LAATDSTSHIEAISQLAKLFDNEKDIQAILMAKTTQDILSVIARY"
     gene            2479..3513
                     /locus_tag="OVW45_004381"
     CDS             2479..3513
                     /locus_tag="OVW45_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCV5528656.1"
                     /translation="MEKTRKRRGTGRVTLQEVANFAGVGTMTVSRALRTPEQVSDKLR
                     EKIEQAVEELGYIPNRTAGALASGHSDTVAVLVPSLTDKASSRFMQSLQQVLNKNEFQ
                     LLLGCHEYNQLKEAEILMTLLQGNPAALVIFGSQLADKTHQILEKTNIPTINVVGSPF
                     SPAQITIETAFFEASHKLTEHLLEQGYKNIGFIGAHMDNRLQRQQLNGWHKAMLSHYQ
                     NSDLVITMPDAASLQLGRYALNEILLRQPELDAVICSHEEIALGIMFECQRRLLKIPG
                     NIAVACLDGSDSCDQTHPTLTSIRIDYKKMGIETGNLLIELLNNNHGDESEESRIEQF
                     NYQIELRQST"
BASE COUNT         1073 a          908 c          846 g          902 t
ORIGIN      
        1 caccgtattg acgcttattt attggtgagt gctacgttcc atggcaggag ttcgtcaaca
       61 cggttggaag gccattccgg cagtacgctc aggatatggc gcagatacgc ttccggatcg
      121 ataccgttca aacggcaggt tggcctcctc cactttttca tgaaagtgaa ggatattaat
      181 gacatcaaaa tattaacgaa cgataatccc taacaaaata cccagagcgc caaagtcagc
      241 atcactgaag gtggtgttag caaaaccaat atcacccagc acgggcaata agaatactgg
      301 caggaaggtg ataagcaagc cttgagcgaa ggccccccaa atcgcaccac gacgtccacc
      361 cgttgcatta ccgaataccc ctgccgctgc acctacgaag aagtgcggca ccacaccagg
      421 aataatcacg gtcatgttca gcagatacaa cgagaacata ccgattaaac ctgcagcaaa
      481 gctgctcagg aaaccaacca gtacagcgtt aggtgcgtaa gggaagacga ccgggcaatc
      541 gagagcaggt ctggcattcg gaaccagctt gtctgaaata cctttgaatg ccgggacaat
      601 ctctgcaatc accatgcgca caccctgcag aatgatgtac acgccagctg cgaaggtaat
      661 ggattgcatg atggagaaca tgaaccagtt tttaccatcg ctaagctctt tcaccacatc
      721 tgcacccgca aacaggcacg tcaccaggaa aattatgctc atggtaaaag agatagcaac
      781 cggcgtgtcg cgcaagaaca gcagactttt cggcacattc atgtcttcag tcgagtgttc
      841 tttattacca aacttgctgc cgataaaacc ggccaataca taagataatg ttgagaagtg
      901 gccgatggca acgtcatcag aaccggtcac tttcttcatg tacggatgcg caatagcagg
      961 gaaaaagacc attgccacgc caacaaccaa agagccaacg gctataagcg taatgccggt
     1021 catgcctgct gttgccagaa ttaccgctac catcatcgac ataaacaacg tgtgatgacc
     1081 ggtcaggaag ataaatttcc acggtgtgaa acgggcaatc agaatgttga tgaccatcgc
     1141 gaagaacata atcatcgcca tttctttccc gaagcttttc tgcgctacgg aaacaatggc
     1201 ttcattgttc ggcacgacac cctgaatacc aaaggcatgc tggaaaatgt ttgcgaagtc
     1261 acccagtgac gacacaacta aacccgcccc tgcgcctaaa atcacaaacc ccataatggt
     1321 cttgacggta cctttaatgc attcggttac cggttttttc tgtgcaatca ggccaatcag
     1381 ggcgattaaa ccgaccagca ctgcgggttc ggaaagtaca tcgctcatca aaaaacgaaa
     1441 gaaatccata tcgcccccag cttacagtgc gcctaattcg gttaatacca cggataaacg
     1501 ttctctcatg gctttcttat caatcatatt ttccagtgcg acgattttgc ctgcgacaga
     1561 ctgcgcgact aactgctcgg caatgtcttt ggtgccgaca tagatatcac tcggcgtccc
     1621 tttcgcggag cccaggtcga cgtgatcaac atcagcgctg acggacaaat ccttcaaaat
     1681 gcttttaatg ctcatttcca tcatcaggct ggtgcctaag ccgttaccgc aaaccactgt
     1741 aattttcata aatgttcctc tggattaata gcgcgcaata acagacaaaa tgtcctgcgt
     1801 cgttttggcc atcaaaatgg cctgtatgtc tttttcgtta tcgaataact tggcgagttg
     1861 agaaatcgct tcaatgtggc tggtactgtc ggttgcagcc agcacaatca gtaatttcac
     1921 tggatcgttt tcatcggcat caaaattcac acctgaaggg atcagggtca gtgctaatga
     1981 cagcttgtta gctccctctt ccgggcgcgc atgaggcatc gcaataccag ggccaaccac
     2041 atagtacgga ccgatagtgt cgtgcgagcg atagatcgca tcaacataac gcggttcgat
     2101 agaaccgttt tcaatcaacg gccggcatga aatcgccacg gcttcccgcc agtctttggc
     2161 ctgttccacg acctgaacga cctctgtatt taatagattt tttaacatgc tactgataac
     2221 tcccaatgat aattaacagg agagtagata ttttacgcat taattaatgt gatgattgtc
     2281 gcatttgata gcgctatctg ataacgctac caatctttgt gatagcgcta tcatttctct
     2341 gccgccatgc tttggctata ttcggttcct gcatatacaa tgcaacagga tatttgctac
     2401 cgtgggcaaa aactggaaag agtaatggtg aaataaaagt aatgaaaacc gctggttgta
     2461 attatccatg gagtcgaaat ggaaaaaaca cgtaagcgcc gtggtaccgg acgggtgacg
     2521 ctgcaggagg tcgctaactt tgcaggtgtc ggaaccatga ccgtttcccg cgcgctgaga
     2581 acaccggaac aggtatctga caagctgagg gaaaagatag aacaggccgt agaagagttg
     2641 ggttatattc ccaacagaac tgctggtgcc cttgcatctg gtcacagtga tacagtcgcc
     2701 gtactggttc cttccctgac agataaagcc agctcccgct ttatgcagtc gttgcagcag
     2761 gtgcttaata aaaatgaatt ccagctactg cttgggtgtc atgagtataa tcagctcaaa
     2821 gaagctgaaa ttctgatgac gcttcttcaa ggcaacccgg cggcgttagt tatttttggt
     2881 tctcagttgg ctgataaaac tcaccagata ctcgagaaaa caaatatacc taccattaac
     2941 gttgtcggtt cccctttcag tccggctcaa attaccatcg aaacagcatt ttttgaagca
     3001 tcacataaac tgaccgaaca tctgctggaa cagggttata agaatatcgg atttatcggt
     3061 gcgcatatgg acaaccgcct gcaacgtcag caactgaacg gctggcataa agcaatgttg
     3121 agccattacc agaactctga tcttgttatc accatgccgg atgccgcaag tctgcaattg
     3181 gggcgttacg cattaaacga gatattactg cgacaaccag agctggatgc tgtgatttgc
     3241 agccacgaag agattgcgct gggtatcatg tttgagtgcc agcggcgttt gttaaaaatc
     3301 cctggaaata tcgctgtagc ttgtcttgac ggttcagaca gttgcgatca aacacaccca
     3361 accttgacat ccatccgtat tgactacaaa aaaatgggga tagagacggg taatttactg
     3421 attgaactat tgaataataa tcatggtgat gaatcagaag agtcacgtat tgagcaattc
     3481 aactaccaga tagaattacg tcagagtaca tgagctcaaa agagcgctgt ggctaggggt
     3541 agtgaccggg tagctgcgat aactgccaat tttcaggcaa ccacctcgat tggcaaccct
     3601 gtttccatat caagttcaaa tatcccgtag aggatgattg gtacctgcct ggcggatttc
     3661 atcatagttc gggataaaaa atcccatcag aaaccggata tatgtaagcg tcaatacggc
     3721 actccgtta
//
LOCUS       DAKISA010000226         3711 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.229, whole genome shotgun sequence.
ACCESSION   DAKISA010000226 DAKISA010000000
VERSION     DAKISA010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3711
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.229"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..1235)
                     /locus_tag="OVW45_004382"
     CDS             complement(<1..1235)
                     /locus_tag="OVW45_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCV5528657.1"
                     /translation="MTSTPITTADIANGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCALINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVDLFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTRVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGV"
     gene            1461..2276
                     /locus_tag="OVW45_004383"
     CDS             1461..2276
                     /locus_tag="OVW45_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCV5528658.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHPARRLESWEIIFVEKGRL
                     TIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDSDPRWLTHLS
                     VPQQTKVVDPQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADGAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
     gene            complement(2308..3003)
                     /gene="araD"
                     /locus_tag="OVW45_004384"
     CDS             complement(2308..3003)
                     /gene="araD"
                     /locus_tag="OVW45_004384"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCV5528659.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDETRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(2997..>3711)
                     /locus_tag="OVW45_004385"
     CDS             complement(2997..>3711)
                     /locus_tag="OVW45_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HCV5528660.1"
                     /translation="WSTAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDDAVRQRA
                     REIMSKAIRLARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWAVEQAAASQVMLA
                     VEIMDTAFMNSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIH
                     LKDTQPVTEQSPGQFRDVPFGEGCVDFVGIFKTLHKLNYRGSFLIEMWTEKAKEPVLE
                     IIQARRWIEARMQEAGFIC"
BASE COUNT          907 a          970 c         1026 g          808 t
ORIGIN      
        1 acgccaggct ggtaggcgac aaaaataaac agcaggctga tgatcccggc gctggaggcc
       61 caggccagct tgatgaaaaa cagattgaag gcgaagttca tgccggaaga acgtacgcgg
      121 gttttccact cgccgtagtc atcggcaaag gccatcaatg agaagtgcaa tggcaatgtg
      181 aagccaagaa tcacgccatt accgaggatc accgccagcc acagcgtttg ataagccggg
      241 ccggagggca ggaaccacat caacaccgcc agtgcagcga ggagcaggtt ggtgtagtaa
      301 taaattttga cggtatcgaa acgccgcgtt aacgggctga caatcaccga gccgataatg
      361 gaggcgaagg tgaccatggt gaagaacagc gacgtgtagc ccgtactgcc ttgtaagacg
      421 taggtaatga agtacatata cccaccgccg cgaatattaa agacgttaat cagcaggaaa
      481 gacatgacca gcatcagcag cagttggtcg ttgttccgca gcccggcaag atgttcgcgc
      541 agggtaaatt tccccattgt ggagagcggc acccgttcac gaacccagaa aaaacagcac
      601 aggaacatca ccacggcaat ggcgcacaat acgccgacgc ccagttgata gccgcgtgca
      661 gcgttgccct gaccgaagag atctaccatc cacggtaagc ctaccgaaac cagaaatccc
      721 gctacgccgc agagaacgaa tcgccaggac tggcaggaga tcacttcatt gtggcgggtg
      781 gtcatggtgt tgatcagcgc gcagtacggt acgttgatgg cggtataacc gactgaaagg
      841 agcaggtaag taccgaacgc ccaggcgatt tttactccca tgctggcatc tggcacggtg
      901 aacgtcagga tgccgataat gccgatggga atggcaatcc atagctgcca cggacgaaag
      961 cgcccccaac gactttgcgt ccggtcggca atgacgccca tcaccgggtc ggagatggca
     1021 tcaaacacgc gcagtgcaat aaacagcgtg ccaaccagcg ccggagttaa tccaaaaacg
     1081 tcggtgtaga aaaaggtgag aaaattcatg atcaggcaag ttattacggt gccgcctgcg
     1141 tcacccaggc cgtagcctat tttttcccgt acagataacg catcgtcaat tttaccgtta
     1201 gcgatatcag cggtagtaat cggtgtagag gtcatagata ttcctcgtga atagggagca
     1261 atttttagtt ttattgaaat gacctgatta attctgccgt gaacgggaat atcaacaagg
     1321 cgagaaaaga gtaaaaaaag gtgacgattc cgacctgaat ttaaaagtgt gaaatggatc
     1381 aggtgaaatt aatgtttttg attaataatc aaaaggtaat gtgtttaatc attaatgaat
     1441 aagcgggaaa ttacatatca atgctcgaat tatccatgac gttaccgatt aaagtgcaaa
     1501 atggtggatt atttatttcc cgcggcgtag ggaggcatcc tgcaaggcgg ctggagtcat
     1561 gggaaattat ctttgtcgaa aaaggtcgat taacgattca ggaagaagaa cggatatttc
     1621 tggtcgatgc gggagagagt ttattactct ggccgaatcg acaacatgtt ggggtggagg
     1681 attttcccgc cgatctcaaa ttttactggc tgcattttga ggttaaagcg ccggatagcg
     1741 accctcgctg gcttactcat cttagcgtgc cgcaacaaac gaaggttgtc gatccgcagg
     1801 cgctggtggc cctgtttcgc cagttcttaa gcgaacagga aaagcatcaa cgcactccgg
     1861 cgctggagtt tattgtgctg ctgattttgc agcagctcac ccgtgacgcg cgtcttgatg
     1921 aacaggccga tggcgcaggc accacccttg cctggaaagc gcagaaactg atccgcacgc
     1981 aatatcatcg cccgctttcc acggcgcttt tagcccgcga gctgtactgc aatgccgatt
     2041 atcttgggcg cgtctaccgg cgggtgtttc atctgacgtt aacagaagca atccatcgcc
     2101 agcgagtgcg cgaagccgaa aagctgttaa ttagcgaagc gcgatcgctc actgaagtgg
     2161 ctgcgttgtg cgggtttaat gacgttggct atttccggca gatttttcgc aaacacaccg
     2221 gtttaacgcc agcagcgtgg aagcggcgtt acagcaagga acatatcaat tcgtagtgcc
     2281 ggggcgatga agccccggcg tgagggatta ctgcccgtaa taggcattgg ccccatgctt
     2341 acgcaggtag tgcttatcca gcagttcgtt ttgcatcgca gggagctgcg gcgcaagctg
     2401 gcgcgagaat aggcccatat aggcgcattc ttcgagtact acggcgttat gcacggcatc
     2461 ggcggcgttt ttaccccatg cgaacgggcc gtgagaatgc accagcaccg ccgggatttg
     2521 tgccggactc ctgccacgtt cttcgaaggt ttcaatgatc acttcgccgg tctgatattc
     2581 atattcgccg ttaatctcct ctgcggtcat ctgtcgcgtg caggggatgg caccatagaa
     2641 ataatcagcg tgcgtggtgc cccaggcggg gagatccagc ccggcctgcg accagatggt
     2701 ggcgtggcgt gagtgggtat gcacaatgcc accgatttcg gcgtagcgac ggtaaagcgc
     2761 cagatgcgtt ggcgtgtcgg acgagggctt tttgctacct tccaccacct tgccgctggc
     2821 tatctcaacc accaccatat cgtcggcggt catcacgtca tactcgacgc cggaaggttt
     2881 gattaccatc cattgccgcg tttcgtctac cgcgctgaca ttgccccagg tgaacgtcac
     2941 cagatggtga gcgggaagcg ccagattcgc cgccagcacg tcggctttca gttgctctaa
     3001 catataaatc cagcctcctg catacgcgct tcaatccaac gccgcgcctg aataatctcc
     3061 agcaccggct ctttggcttt ttcggtccac atctcaatca ggaaagaacc gcgatagttc
     3121 agtttatgca gcgttttgaa gatgccaacg aaatcgacgc agccttcgcc aaacggcacg
     3181 tcgcggaact gtccgggact ttgctcggta actggctggg tgtctttcag gtggatcgcg
     3241 gcgatacggt caatgcccag tttcagttcg gcgggaacat cattgcccca ggcgctgagg
     3301 ttgccgacgt ccgggtagac ggtgaaccac ggcgaggcga gcatctcgtc ccactttttc
     3361 catttactga tggagttcat aaacgcggtg tccataatct ccaccgctag catgacttgt
     3421 gatgccgccg cctgttcgac cgcccacgcc agcccttcag caaaacgttg ccgggtgcct
     3481 tcatcgtggt cttcgtaata gacgtcgtag cccgccagct ggatggtgcg tatgccgaga
     3541 tcgcgtgcca gacgaatcgc tttgctcatg atctcccgtg ctcgctggcg taccgcgtcg
     3601 tcacggctac caaaaggaaa gcgacgatgt gcggacaggc acatcgacgg gatcccaaca
     3661 cccgtttcga tcatcgcggc aaccagcgaa gtcctttgtg cggtgctcca g
//
LOCUS       DAKISA010000227         3627 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.230, whole genome shotgun sequence.
ACCESSION   DAKISA010000227 DAKISA010000000
VERSION     DAKISA010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.230"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(89..652)
                     /locus_tag="OVW45_004386"
     CDS             complement(89..652)
                     /locus_tag="OVW45_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCV5528661.1"
                     /translation="MNRTSIEKLPSLTHNHLPVMTTELLADLYGTERQRLTNNFNRNK
                     ERFIEGKHFFLIEGDALRELKNENSLRVSVKIARNVRSLILWTERGAARHAKMLETDQ
                     AWEVFEKLEDCYFNQKQPPAAQNTSIENDGCALLSHFDKHGQVEFTEKVPADAMVCTL
                     ERFKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(756..974)
                     /locus_tag="OVW45_004387"
     CDS             complement(756..974)
                     /locus_tag="OVW45_004387"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528662.1"
                     /translation="MGELKSSPVGFPVVPTLCVSPPDDWNLTVLTVSGLQFTFPRGAQ
                     NTSKAVSTPDSHVVFLCPEFLVYDRVCG"
     gene            complement(1133..1519)
                     /locus_tag="OVW45_004388"
     CDS             complement(1133..1519)
                     /locus_tag="OVW45_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCV5528663.1"
                     /translation="MARDIQQVMERWGAWAANTHEDVSWASIAAGFKGLIPSKVKSRP
                     QCSDDDAMIICGCMARLNKKNQDLHDLLVDYYVGGMTFMSLARKHGCSDTCIGKRLQK
                     AEGVIDGMLMMLDIRLEMDRYVERIM"
     gene            complement(1509..1880)
                     /locus_tag="OVW45_004389"
     CDS             complement(1509..1880)
                     /locus_tag="OVW45_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCV5528664.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKVGERYRRDVTLIVRQQ
                     RLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQ
                     LVPGGRLGVKIYEITGDNDGA"
     gene            complement(1880..2137)
                     /locus_tag="OVW45_004390"
     CDS             complement(1880..2137)
                     /locus_tag="OVW45_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528665.1"
                     /translation="MNNQTMTFTSEQLRKHAQEMLRQAEQLEKTGVTKDAIRRDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETKVGKFEKMVQEVLR"
     gene            complement(2134..3525)
                     /locus_tag="OVW45_004391"
     CDS             complement(2134..3525)
                     /locus_tag="OVW45_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCV5528666.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADHEVMDILITLPADIFSVRA
                     YRDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWARFSQGVGRMTG
                     RNIWMVDQANLTIDEICATTKHHLIKHPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQATGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESAQVLSTPMRQPQPRRYS
                     NTRTDSSKMERFF"
     gene            complement(3522..>3627)
                     /locus_tag="OVW45_004392"
     CDS             complement(3522..>3627)
                     /locus_tag="OVW45_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCV5528667.1"
                     /translation="FISPRIADRITDGGRNWLSFNWPSYRSCIRGVAA"
BASE COUNT          850 a         1013 c          811 g          953 t
ORIGIN      
        1 catcggcggg tttaagctgt gtggcgtagt aaccactctt aacaggatat tcaacttttt
       61 acgatcgtaa agttttcgga aaaaattttt aaaaccgcat caaccgctcc accagttgct
      121 ctttacgggc aacgatccac ccgtgttgct ccagataaaa tttaaaccgt tccagagtac
      181 ataccatcgc atcggcgggt accttttccg tgaactcaac ctgaccgtgt ttatcgaagt
      241 ggctcagtaa tgcgcatcca tcattttcga tagatgtgtt ttgtgccgct ggtggctgtt
      301 tctggttgaa ataacagtct tccagttttt cgaacacttc ccacgcctga tcggtttcga
      361 gcattttggc gtgacgggct gcgccacgtt ctgtccagag gatgagggag cgaacgttac
      421 gggcaatttt cacagaaact cttaaagagt tttcgttctt caactctcgt aacgcatcac
      481 cttctatcag gaaaaaatgt ttaccttcta taaaccgttc tttgtttcga ttgaaattgt
      541 ttgtcaaacg ctggcgctct gttccgtaca aatcagccag cagctctgtt gtcatgacag
      601 gaagatggtt atgcgtaagt gacgggagtt tttcaataga cgtgcgattc atagacatgt
      661 cctttcgaat attttgattt accccttttg agagggtgac cgggcgctca aaaccgtcga
      721 aagtcggcgg gcgtatttcc attgctggta ttgtattagc cgcacacccg gtcataaacc
      781 aagaattctg gacacaaaaa aaccacatgg ctatcgggtg tggataccgc tttcgaggtg
      841 ttttgagcac cgcggggaaa tgtaaactgc aaacctgaaa ctgtcaacac cgtcaggttc
      901 caatcatcgg gtggtgagac gcacagggtt ggaactaccg ggaaaccgac cggcgagctt
      961 ttcagctccc ccatgcgccc caccataatt cagatgtgcg cgtgcatacg acaataaaaa
     1021 acacgctcgc ggcgtgtgtc tgtcgcggtc tctatccggg gttccaatcc cgacgccaga
     1081 ttttgctggc gcgtgaggaa tatagccccg gacaatgtgt ctggtcaagc acctacataa
     1141 ttcgttctac gtatctgtcc atctccagcc ggatatcaag catcatcaac atgccatcaa
     1201 taaccccttc cgctttctgc aggcgcttgc caatacaggt atccgaacac ccatgtttcc
     1261 gtgccagact cataaaagtc attccaccta cgtaataatc caccagcaaa tcgtgcaaat
     1321 cctgattttt cttgttcaac cgggccatac agccacaaat tatcattgca tcatcatcag
     1381 aacactgagg gcgtgatttc actttcgacg gaattaaccc tttaaaacca gcagcgatcg
     1441 acgcccagga tacatcttca tgagtgtttg cagcccaagc cccccaccgt tccataacct
     1501 gctgaatatc acgcgccatc gttatcacct gtgatttcat aaatcttcac gcccagtcgc
     1561 ccaccaggaa cgagctgacc gcgcacaata ttgatttcat caaactgctc gtcgtctatg
     1621 agtagtccgg catgcgtcag cgcatccagt ggtgctttca ggatattgtc caggtcacga
     1681 cggcgcttat ccggtggctc tgcaataatc tttatcgcca gccttccgga caggtttaat
     1741 ttcagccgct gctggcgaac aattagcgtc acatcacggc gataacgctc accaactttt
     1801 gatacaaaat atgtgctgcc acgacgtcgc cagtaagtat tcaccgtcgg cggataaggt
     1861 aaaataaact catggcgcat cagcgcagca cctcctgcac cattttttca aactttccaa
     1921 ctttggtttc cagctctgcc acacaatcaa cgagctcatc caccgctttt tgtgcgcgat
     1981 gtttcgcctg catcagttcc ctgagcgctg gcaccatatc ccgacgaatg gcatcttttg
     2041 ttacacctgt tttttccagt tgttccgcct gtcgcaacat ttcctgcgcg tgtttacgca
     2101 attgttcaga ggtaaaagtc attgtctggt tgttcaaaag aaacgctcca tcttactgct
     2161 gtcggtacgt gtattgctgt atctgcgcgg ctggggctgc cgcattgggg tggaaagaac
     2221 ctgtgcgctt tcctggtcca caggcagaaa atgtccgtta taaaaacgcc ggtaaatcgt
     2281 ccccagagaa ccgttacgtt gtttcgtgat attaatttct gctatgcctg tagcctgtgt
     2341 atccgggttg tacacttcat ccctgtaaag catcagaatg atgtctgcat ccgcctctat
     2401 ttctccggaa tttttcaggt ctgagttcat gggacgttta ttgggtctgg actccacacc
     2461 gcgggagagc tggctcagcg caatcaacgg aaaaccaccg gattttgcca ggcctttaag
     2521 cccctttgag atttcaccca cggcaaggtc atgacgcccc gtggttcggg tttttatcag
     2581 cccgagataa tcaaccacca ccagcgccgt ttccggatgt ttaatcagat ggtgtttcgt
     2641 tgttgcgcat atctcatcaa tggtcaggtt cgcctggtcc accatccaga tattgcgccc
     2701 ggtcatccgc cccacccctt gtgagaaacg cgcccagtct tcatcttcaa agtgagccac
     2761 agatttcagg cgtgatactg gcatccctcc agccgcagac accatacgtt caccaatctg
     2821 gatgttcgcc atctccatgg tgaacagaag cacaccatgc ccctgctcag tcaccttgtc
     2881 gatgatgtcc agcgcaagtt cggttttccc catcgaagga cgggcggcaa tgaataccag
     2941 gtctccgggc tccataccgc ctgtttttgc gtccagttca tcaataccgg tcatcaacgt
     3001 cctggatttc tccagcccct gattccggca ttcaacacgc tcaaccactt ccggaagcac
     3061 atcatcaata tgtaccggct gaatgacgcc ctttcctgtc gacagtgtga ccatcatgtt
     3121 ctgcgcatcc ttcagggcat cttcagctgc ttcacaggta tgcgcatcac gtaatttctg
     3181 cagcgcctca ttcagtgttt tttctgcatc gcgcagtgcg gcattgcgcc gcaacgctgc
     3241 aacatagtgc tccagtgaag acttcaccca ggttttacgc ccggtatcag taatcaccgg
     3301 ggcaagttcc ggcatctcat tacacaacag cacggggtca atcactcctg aaacacgggc
     3361 ctgtctgcag atgcctgtgt agatatcccg atacgctcgt acagaaaaaa tgtccgccgg
     3421 tagtgtgatc agaatatcca tcacttcatg atctgcccca cgcagaaaga atgcgccaat
     3481 gacagcgcct tccaggtcat cattacgcca gactggtgtt gtcatgcagc cacacctctg
     3541 atacaagaac ggtagctggg ccagttgaac gacaaccagt tgcgcccccc gtctgtgatc
     3601 ctgtcggcaa tgcgggggct gatgaac
//
LOCUS       DAKISA010000228         3586 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.231, whole genome shotgun sequence.
ACCESSION   DAKISA010000228 DAKISA010000000
VERSION     DAKISA010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.231"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..123)
                     /locus_tag="OVW45_004393"
     CDS             complement(<1..123)
                     /locus_tag="OVW45_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCV5528668.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPY"
     gene            384..719
                     /gene="iraM"
                     /locus_tag="OVW45_004394"
     CDS             384..719
                     /gene="iraM"
                     /locus_tag="OVW45_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCV5528669.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1000..1131)
                     /locus_tag="OVW45_004395"
     CDS             complement(1000..1131)
                     /locus_tag="OVW45_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HCV5528670.1"
                     /translation="MIAYCFCEWHCSSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(1631..1707)
                     /locus_tag="OVW45_004396"
     tRNA            complement(1631..1707)
                     /locus_tag="OVW45_004396"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1671..1673),aa:Arg,seq:tct)
     gene            complement(1808..1883)
                     /locus_tag="OVW45_004397"
     tRNA            complement(1808..1883)
                     /locus_tag="OVW45_004397"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1848..1850),aa:Ile,seq:cat)
     gene            complement(1986..2840)
                     /locus_tag="OVW45_004398"
     CDS             complement(1986..2840)
                     /locus_tag="OVW45_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCV5528671.1"
                     /translation="MKLEDLPKYYSPKSPCLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESVVFTNKVQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTRLIF
                     PESVVNSS"
     gene            complement(2837..3211)
                     /locus_tag="OVW45_004399"
     CDS             complement(2837..3211)
                     /locus_tag="OVW45_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCV5528672.1"
                     /translation="MRHEFILPYPPTVNTYWRRRDNTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMHEEQVKK"
     gene            complement(3211..3468)
                     /locus_tag="OVW45_004400"
     CDS             complement(3211..3468)
                     /locus_tag="OVW45_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528673.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEVLR"
BASE COUNT         1009 a          949 c          685 g          943 t
ORIGIN      
        1 ataaggggtt atggccaccg gactaccaaa cggctgccag ccctctttca gtttatgtgt
       61 cagcttttcc gcaagatctg acggcggcgc cgccctgaca acatcatagt gtttaaatgc
      121 catggttctt tccaccatct gaaaaataat tctttaaaat acctgacatg taatacagaa
      181 aaaacacaaa accatacctt aaataaaaac ctgattatca agcagatatg catggataaa
      241 ctacaagacg agatataaac caccctgcat ttaaataaac aataaacaac atcagaaaaa
      301 taattctgct ctatggttta cattcaaaaa tatcatttat acttttcaga acatcaccag
      361 caaggcataa acaaggaaac taaatgaagt ggattgtgat tgatacagtt atccagccat
      421 catgcggaat atctttttca gtcatatgga gtaaaataaa attaataatc tggtatcaat
      481 cggatgcttt cttacctcct gaaagtatat ttacactgac tcacacaggc atcatgctca
      541 ataacaaagt gctacctgta accatttaca acgtagtacc attcaataaa acattctgga
      601 atttaatcaa aaacagccag gaatgcccta caaatacaga taacgtattg aatgaatgct
      661 ttaataaccg ttgcactctg caaatatgtc cttatggact aaaacaacaa agtccataag
      721 gagtttactc acatctgaca aaatcaatat aaacagcccc tccggagagg ggctggagag
      781 tggcgctatg tgccattgca tggtgccggg tgcctcccgg tgaattcagt accagcacct
      841 gaatccgcga ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac
      901 catgccagtt tcttttaaca aactccccgc aaaccagaca acagtcaacc gcctgaattg
      961 tgaagtattt aaaaatttct cccgctaact gatacccggc taacagtctg gcgttttctt
     1021 tttcagcaac gggaaagcaa caaccaccac acccgccacc agcacaccgt cagccagcac
     1081 tgacattatc cggctgctgc aatgccattc acaaaaacag taagcaatca ctttttaccg
     1141 taacaggtga taatccagat atgtatctac cccagatgag taatccgaag ttcatccata
     1201 ccacaggtcc tggctattct gttgtactcc tgaacaagag caaataattc tgaattagca
     1261 accatgaact catcgcaaac cctctgtata gcatcactat tcagaataat aacgtctctt
     1321 cccgaaagac gatcaggagt acagaacaaa actgtcaaac ggctgaaggc ctttgctcgt
     1381 gctgcattga ctatatcaat acgctgccta aggatgaaac accccgacgc ctcatcaata
     1441 ttcactctac ccacaccata tgaatgataa atatttaatg ctgaaaaaac cattagaccg
     1501 tataacaaac actcaatcaa tacttaacag aacttttatt tttgacaaac ataaaatatt
     1561 ttcaacaata tcctgagcca ggtatatttc agtataaggc tctgccggaa ggaatctgga
     1621 agaatgaata tggcgcgctg tactggattc gaaccagtga ccgattgctt agaaggcaat
     1681 tgctctgtcc ggctgagcta acaacgctga ataccgataa tggaccgcca tcggggaccc
     1741 gcccccgcac caacaaccct gttatcgtgt cgtctgctct tcctgataag ctaatggcgg
     1801 tttgtgatgg tggcccttgc tggatttgaa ccagcgacct ggcgattatg agtcgctcgc
     1861 tctcaccact gagctaaagg gccgggagca gaataataac ggtccgtaat taattccgca
     1921 ataaaaaacc cgctcggcgg cgggttgtag aaactcttct aacgtcaggc ataaaacgcc
     1981 catagttatg acgaatttac cacagattcc ggaaaaatca acctagttac cttttttaac
     2041 tgccgctcag cccatgcttc ttcaatatca aaccgggtca ccagcgcatc atagaatttc
     2101 ttaactgttt tttcccatga cgcgcgtgtt atctggtttg tcacctcgca tatagcatta
     2161 aatgcctccg ttgatggtag tctttcatag ccacgaccac cacaacgctg gcagtctctg
     2221 ataacaggca taccacgttt taccgactct tcacgatgaa tggcgacacc acgcccacgg
     2281 caatccttac aggcggtgga aacctcaccc tttccgccac actccggaca ggcaactttt
     2341 accacctctc tgactttttt ccattcttcc cagtaagacg gatacacacc tttcgtacac
     2401 tttgcccata ccggcggctt accatccgga tactggacct tgtttgtaaa aactacgctt
     2461 tcaataaatt tttccccata gcaacaaggg cactgctttt tactcgctgc gctgcgggca
     2521 taatcctcaa aagcgtacga agccataatg cgcatcacta ccggttttat ttctgccgga
     2581 agttttctca acgccgccac acgatcgcac cgactgagtg cataatctgc cagtaattct
     2641 gttgcccgcg ccctgtcatt catactgatg cccattttcc ccaggaacgc agaaaacccc
     2701 atctcagccc gattctgtgt catgccctgc gcggccatca catcagtgat actcagcgca
     2761 tcttttgacg ttgaggccga tgcatcggtc aggcaagggg attttgggga gtagtatttc
     2821 ggtaaatctt ccagtttcat tttttgacct gctcttcatg cattatgggg taaatcttca
     2881 cccccagacg tccaccagat actggctgac cacgaacgat attgatttca tcaaactgct
     2941 catcgtccat taacactccc gcatgtgtca gtgcatccag tggtgctttc aggatattgt
     3001 ccaggtcacg acggcgctta tccggtggct ctgcaataat ctttatcgcc agtcttccgg
     3061 acaggcttaa tttcagtcgc tgctgacgga caataagcgc cacatcccgg cgataacgct
     3121 caccggcttt tgatacaaaa tatgtgttgt cacgacgtcg ccagtaagta ttcaccgtcg
     3181 gcggataagg taaaataaac tcatggcgca tcagcgcagc acctcctgca ccagtttttc
     3241 aaactttccg actctggttt ccagctctgc cacacagtcc accagctcat ctactgcttt
     3301 ctgtgcgcga tgcttcgcct gcatcagttc ccgaagcgcg ggtaccatat ctttacggat
     3361 agcgtctttt gttatgcccg ttttttcgag ttgttcagca tgacgcagca tttcctgcgc
     3421 gtgtttacgc aattgttcag gggtaaaagt cattgtctgg ttgttcaaaa gaaacgctcc
     3481 atcttactgc tgtcagttcg tttattactg tatctgcgcg gattgccggg cttcatggga
     3541 gtggaaagca cccgtgcact ttcctggtcc acaggcagaa aatgtc
//
LOCUS       DAKISA010000229         3514 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.232, whole genome shotgun sequence.
ACCESSION   DAKISA010000229 DAKISA010000000
VERSION     DAKISA010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.232"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            87..1333
                     /locus_tag="OVW45_004401"
                     /pseudo
     CDS             87..1333
                     /locus_tag="OVW45_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
     gene            complement(1862..2062)
                     /gene="torI"
                     /locus_tag="OVW45_004402"
     CDS             complement(1862..2062)
                     /gene="torI"
                     /locus_tag="OVW45_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HCV5528674.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(2185..2529)
                     /locus_tag="OVW45_004403"
     CDS             complement(2185..2529)
                     /locus_tag="OVW45_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528675.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            2771..3373
                     /locus_tag="OVW45_004404"
     CDS             2771..3373
                     /locus_tag="OVW45_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528676.1"
                     /translation="MSYFLCKKWMINLSGAGKILWALNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGICIFSGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
BASE COUNT          872 a          703 c          803 g         1136 t
ORIGIN      
        1 caagaactgg aatagtgaga agtaaacggc gaagtcgctc ttatctctaa ataggacgtg
       61 aattttttaa cgacaggtag gtaattatgg cactgaatat tccattcaga aatgcgtact
      121 atcgttttgc atccagttac tcatttctct tttttatttc ctggtcgctg tggtggtcgt
      181 tatacgctat ttggctgaaa ggacatctag ggttgacagg gacggaatta ggtacacttt
      241 attcggtcaa ccagtttacc agcattctat ttatgatgtt ctacggcatc gttcaggata
      301 aactcggtct gaagaaaccg ctcatctggt gtatgagttt catcctggtc ttgaccggac
      361 cgtttatgat ttacgtttat gaaccgttac tgcaaagcaa tttttctgta ggtctaattc
      421 tgggggcgct cttttttggc ctggggtatc tggcgggatg cggtttgctt gacagcttca
      481 ccgaaaaaat ggcgcgaaat tttcatttcg aatatggaac agcgcgcgcc tggggatctt
      541 ttggctatgc tattggcgcg ttctttgccg gcatattttt tagtatcagt ccccatatca
      601 acttctggtt ggtctcgcta tttggcgctg tatttatgat gatcaacatg tgttttaaag
      661 ataaggatca ccagtgcgta gcggcggatg gggaggggta aaaaaagagg attttatcgc
      721 agttttcaag gatcgaaact tctgggtttt cgtcatattt attgtgggga cgtggtcttt
      781 ctataacatt tttgatcaac aactttttcc tgtcttttat gcaggtttat tcgaatcaca
      841 cgatgtagga acgcgcctgt atggttatct caactcattc caggtggtac tcgaagcgct
      901 atgcatggcg attattcctt tctttgtgaa tcgggtaggg ccaaaaaatg cattacttat
      961 cggtgttgtg attatggcgt tgcgtatcct ttcctgcgcg ctgttcgtta acccctggat
     1021 tatttcatta gtgaagctgt tacatgccat tgaggttcca ctttgtgtca tatccgtctt
     1081 caaatacagc gtggcaaatt ttgataagcg cctgtcgtcg acgatctttc tgattggttt
     1141 tcaaattgcc agttcgcttg ggattgtgct gctttcaacg ccgactggga tactctttga
     1201 ccacgcaggc taccagacag ttttcttcgc aatttcgggt attgtctgcc taatgttgct
     1261 atttggcatt ttcttcctga gtaaaaaacg cgagcaaata gttatggaaa cgcctgtacc
     1321 ttcagcaata tagacgtaaa cttttccggt tgttgtcgat atctccctat ccctcaaccg
     1381 gaaaataata atactaaagt gcttagcccg gctaataatc acctaatcca aacgcctcat
     1441 tcatgttctg gtacagtcgc tcaaatgtac ttcagatgcg cggttcgctg atttccagga
     1501 cattgtcgtc attcagcgac ctgtcccgtg tatcacggtc ctgcgaattc atcaaggaat
     1561 gcattgcgga gtgaagtatc gagtcacgcc atatttcgct atcaggattc tgtgtgatgg
     1621 ttacatcgcc cggcccaggg ctgtttagtc atcagcgctt tctgacagtg ctgagatttc
     1681 aacctgttgc agtaaaaatg agtagatata aggcaagtgt gctgccaaac ccatctttta
     1741 cggggtgaag gtagatttcg tttgaagggt atctggtgtc ccctgcagac atctacttga
     1801 ggcggcaggg gattgattgg aatgctgttt tttagatttg aaaaatattt tacccgctat
     1861 tttacccatt ggcgcggctt aagagcttat ttttgaattc acaatggtca cgatataacc
     1921 atcttgctcg tccgtggata actttggctt taggcaggtc gccggactta atccggtcat
     1981 agatgaaggt cttaccgaag ccagtatcag ccatgatgaa tttcaaatca accagtgaat
     2041 caggttgtag ttcgtgttgc atgagtgcta tctccgaata tggaatcgaa cctgcaaatc
     2101 aggcaataaa aagccgcatt gatgcagcaa tggtaggtct ggatatcttg agaaatgaac
     2161 aggcctcatc gagtgtgagg cgggttagtc cttgcgtagc tcgctgattc ttctgtaagt
     2221 ctctggtgct ttgttcccgt acgtcttcat ttcagacttc aacagagcaa cgagtgaatc
     2281 ccattcgttg aggattcctt tgaatgccgg aacgcgcttt gcaaccttgt cgaatgaatc
     2341 tctgatttct ggaatctgct caacaagtgc aacgcatcgc cgaaagtctg ctgcgtcatg
     2401 gggagcgccg aagtgatgac catagatatt ctttttcagg ccacatgcga ttgaggcaag
     2461 agttgcgcta ctgatgccga catcgccagt tgattgccat ttcaaaacct tcatagccaa
     2521 atctgacatt tcttgtctcc ataaaacaaa actcgccgta gcgagctcag ataaaagaaa
     2581 tccccgtcag tgcgaggatg ctgttcattg ctgctataca cttttttgct ctcaacgtaa
     2641 gcggtagccc attctgttgg gttggtcagt tgcttttagg aaatgctctt taccccttaa
     2701 acgtcggctg aaagagctaa aatccatgca aaaaatttac gcaattttgt gtattattgt
     2761 gcagtaagta atgagctatt ttctgtgcaa aaaatggatg ataaatttgt ccggggcagg
     2821 aaaaatttta tgggcgctaa acatgaaaaa agattcgtat ccttatttga tttgcatgac
     2881 agtttcaggg ctgatcttta ttttcctttt cttctggtgg cgggcagata tctacagggt
     2941 cacgtttctt aatcagagta tatcccacta ttacattctg tttagcatgg gaatagcttt
     3001 tctgttatct ctgttttggg ttaagaaggg gatagtaaaa caaagcggct ggaagagtct
     3061 gtcagcatac cttaaggttt atgcagggat atgcatattt tctggatttt ttctgattat
     3121 accccttaca acactaactt attttttgcc tggagagaca tcgtcttatg ttgcaccgta
     3181 tcggtatact tccggtagtt caaaaagttg ttctggagct gaggtggatg accccgatct
     3241 acatgagaat attcgcattt gctatccgta tggcaattat gagtacgata atattatcta
     3301 tgttgaaaag aaaattaata cattaggtgc ggtagtgaca tatgcacaga ccgcgcgtga
     3361 tgatactgaa tgagatagta tatagcgggc aagttttagt taatttatcg aggtaatata
     3421 atttacctcg actcgtttat tctggtatga atatttcgct ttacgatcga tttttatctg
     3481 atgatatcat gcggttttca tatactgact tact
//
LOCUS       DAKISA010000230         3510 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.233, whole genome shotgun sequence.
ACCESSION   DAKISA010000230 DAKISA010000000
VERSION     DAKISA010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.233"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(39..1718)
                     /gene="bcsG"
                     /locus_tag="OVW45_004405"
     CDS             complement(39..1718)
                     /gene="bcsG"
                     /locus_tag="OVW45_004405"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCV5528677.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFATFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGASVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1715..1906)
                     /gene="bcsF"
                     /locus_tag="OVW45_004406"
     CDS             complement(1715..1906)
                     /gene="bcsF"
                     /locus_tag="OVW45_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCV5528678.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1903..3474)
                     /gene="bcsE"
                     /locus_tag="OVW45_004407"
     CDS             complement(1903..3474)
                     /gene="bcsE"
                     /locus_tag="OVW45_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCV5528679.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
BASE COUNT          901 a          883 c          890 g          836 t
ORIGIN      
        1 caggccgcag aggttaaaat ctgcggcctt tttcgtgctt actgcgggta aggcacccag
       61 tcgccgccgt tcaggcgaac gtacggttta tcctggtatt gaataactac tgcatttgag
      121 ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg
      181 tcttcggtga aaatcttgcc atcaagaacg cgaaccacca gatcggagat agctaggaag
      241 ctgctcggtt ggtcaatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat
      301 ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag gccagatacc
      361 tgcattctgt cgcccttcag cgcgccgccg tgttccggca ccacgaccac catcacttta
      421 cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc
      481 cgcgctttgt aatccgctgt tttgctgacc cccggataat ggttgccgtc atgcagtgga
      541 agcgtgttgt agaacgtggc actacggctg tttttatctt tttcggtaac gtccagccag
      601 cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc
      661 ggcagatttg tttgatccat caattcagtc tgcatgccgc cattttcgcg aacttctttc
      721 aaaaaaccgc cgaactggcc gttatgtccc atcatcaggt gctgggtaaa gcccagtttc
      781 gaaaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa
      841 gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactgta ggaggtggcg
      901 gagttaaagt tcttgaactc aatatcgaaa tgcgaccaca gtggatgcga catcaacccg
      961 gcggcttcta tatccgacca ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc
     1021 tgagcatcag cgggcagcga agacgggaag gtcgatttac gtttcgcctc cgcgttatag
     1081 aaattattca gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata
     1141 tcacccacta ccgatgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc
     1201 gttgttacag tggtcgtcgg ttgtccggct ggccacaagg agaaacttgg tcccgccagg
     1261 gtaagtacgt tcagccatag cagtatggca accacaaaaa cggtaatgcg aatccattgt
     1321 gacaggaata accaggccac taataaaaca aaaatggccc caatcatctg ccagttaata
     1381 aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg
     1441 ctcattatgc tttccgggcc aggcaaccag gtgtcatgcc agaacaaagc aaagccgatc
     1501 ggcagggcaa tccagtggcg caagcgatgc aggctgtagc gcggaatggg catcagcaga
     1561 aacgtggcaa acaccaaatt gaggagcgga tggaagttaa gatatcccgc ccacaacagg
     1621 ccgaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta ttgccagagg
     1681 gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt
     1741 ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag aacaaacgta
     1801 aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg aaaaatatca
     1861 gtgcgcaaac gacaataatt tcaatgatat cgctgatggt catcatgatg agcgctccac
     1921 agcatcatct aacagtcgca tgggttctgg tattcgtcgc cagtggcgac catcgtgctc
     1981 ggcattgatg accggtttag aactttgcgt taaaggcagc ggcatgcccc attgttctgg
     2041 ggcaagcaag cgcatctgca ccagctcggc actgatttga tcatcttcaa accagaccat
     2101 acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag
     2161 atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat
     2221 atcgccggta cggttagggc gacacagcgt cagggcttgt tcaacgcgga tacccggtac
     2281 cggacgtagg gcaaccagaa cgcctttacc gtgggcaggt aatagagggt tattcatcat
     2341 gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg
     2401 ggtcattgac agcaaggtag tgatatcttc cggcacatag cgactaaact tctgcccttg
     2461 cacgctttcg atcatcgtca gacaacggga gagtggcgca ttccacggaa taaccatatt
     2521 tgcaccgcag gccaataaca aacgttcatc ggtggcgcgc aggctagcgg tattttcccg
     2581 cacgaggatt ttcatcgcac taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag
     2641 tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg
     2701 ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg
     2761 cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat
     2821 ctccgcctct tcgctttgaa ctaatgtcca gataccattt tgttgctgaa cgctaagctg
     2881 ctgacgggcg ctgacccctt tttcgttgca ccagaaggca atatccagca aatgttggtc
     2941 accctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctcaa gcaacaatga
     3001 aaattgttta tcgttattat ttccgggatt aattaccaaa agcgaacaat ggtttaacct
     3061 gctccattta ttcattttat ccaaccatga gcgaagccgc tcggcaggaa tgttttgcca
     3121 tgcgttattt gcgcaaacaa gaataaaaag gtaattatgg ggatcaatag aacactgcaa
     3181 atcacgggcc aaatagtata gacctttttc atgatttagc attgaaaata attttatttt
     3241 ttccggccct tgagaatcat ctaattgaaa gattttcgcc ggatcgctgt ccatgctaat
     3301 gaccgcgacg tgtgcggttt cagcctggga tgcaattgtt tgattcgcca gactgatagc
     3361 atcttcatgg cgatcgacgt taaaccacca gacgccgcct gctggcatat gtcgcagctc
     3421 atcccataat gatgagatac cgatagagaa tacagggtcc acaatgtccc tcatgtcacc
     3481 attttgtctg attatcagtt tactagcgaa
//
LOCUS       DAKISA010000231         3457 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.234, whole genome shotgun sequence.
ACCESSION   DAKISA010000231 DAKISA010000000
VERSION     DAKISA010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.234"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..934
                     /gene="flhB"
                     /locus_tag="OVW45_004408"
     CDS             <1..934
                     /gene="flhB"
                     /locus_tag="OVW45_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HCV5528680.1"
                     /translation="DPNLILGQIILLIREAMLALLPLISGVVLVALISPVMLGGLVFS
                     GKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTGFYLWHHWPQMMRLM
                     AESPTTAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKKLRMSRQDIRDEFKQ
                     SEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVALQYDENKMSAPKVVA
                     KGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQLYAAVAEVLAWVWQ
                     LKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            927..3005
                     /gene="flhA"
                     /locus_tag="OVW45_004409"
     CDS             927..3005
                     /gene="flhA"
                     /locus_tag="OVW45_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HCV5528681.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            3005..3397
                     /gene="flhe"
                     /locus_tag="OVW45_004410"
     CDS             3005..3397
                     /gene="flhe"
                     /locus_tag="OVW45_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HCV5528682.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
BASE COUNT          740 a          847 c         1038 g          832 t
ORIGIN      
        1 tgacccgaat ctgatcctcg ggcagattat tctgctgatc agagaagcca tgctggcgct
       61 gctgccgctg attagcggcg tggtgctggt ggcgctcatt tctccggtca tgctgggggg
      121 gctggtattt agcggcaaat ccttgcagcc gaagttttcc aaactcaacc cgctaccggg
      181 cattaaacgg atgttctcgg cacagactgg cgcggagttg cttaaggcaa ttttgaaaac
      241 catcctggtt ggcagcgtga cggggtttta tctctggcat cactggccgc agatgatgcg
      301 cttaatggcc gagtctccga ctaccgccat gggtaatgcg atggatctgg ttgggctatg
      361 cgcactgctg gtggtgcttg gtgtcattcc aatggtggga tttgacgtct ttttccaaat
      421 cttcagccac ctgaaaaagc tgcgtatgtc gcggcaggat attcgtgatg agttcaaaca
      481 aagcgaaggt gaccctcatg ttaaagggcg gatccgtcag atgcagcgag ctgctgcacg
      541 gcgtcggatg atggccgatg tgccgaaagc tgatgtcatt gtcaataacc cgacccacta
      601 ttcggtagcg ttgcagtatg acgaaaacaa aatgagcgca ccgaaagtgg tcgctaaagg
      661 tgcagggctg gtcgcgctgc gcattcgtga aattggcgct gaaaataacg tcccgacgct
      721 tgaagcgccg ccgctggcgc gagcgctgta tcgacatgcg gagattggtc aacaaatccc
      781 gggtcaactg tacgccgcgg tggcggaagt gctggcctgg gtctggcaac tgaaacgctg
      841 gcgtctggct ggtggacagc gccctgtaca acctactcat cttccggtgc cggaagccct
      901 ggattttatt aacgagaaac cgacccatga gtaatctggc cgcgatgctg cgcctgcccg
      961 caaacctgaa atcgacacaa tggcagatcc ttgccggacc gattttgatc ctgttgatct
     1021 tgtcgatgat ggtgctgcca ctgcccgcat tcatactcga cctgttgttt accttcaata
     1081 ttgccttgtc gatcatggtg ttgctggtgg cgatgtttac ccagcgcacg cttgagtttg
     1141 ctgcgtttcc gaccattctg ttgtttacta cgttgttgcg tctggcactt aacgtggctt
     1201 caacccgtat cattttgatg gaagggcata ccggcgcggc ggcggcaggg aaggtggtcg
     1261 aagcgttcgg tcacttcctc gttggtggca atttcgctat cggtatcgtg gtgtttgtca
     1321 ttctcgtgat catcaacttt atggtcatta ccaaaggtgc cgggcgtatc gcagaagtgg
     1381 gtgcgcgctt tgttctcgat ggtatgccgg gtaagcagat ggcgattgac gccgacctta
     1441 acgctggatt gattggtgaa gatgaggcga aaaaacgccg ctccgaagtg actcaggaag
     1501 ccgattttta cggctcaatg gacggggcaa gtaagtttgt tcgcggcgat gccatcgccg
     1561 ggatcctcat catggtcatt aacgttgtcg gcgggttgct ggtcggcgtg ctgcaacatg
     1621 gcatgagcat gggacacgcg gcggaaagtt atactctatt gaccattggc gacggtctgg
     1681 tggcacaaat tccggcgctg gtgatttcta ccgccgcggg ggtcatcgtt acgcgtgtca
     1741 gcaccgatca ggatgttggc gagcagatgg tgaatcagct tttcagtaac ccaagcgtta
     1801 tgttgttaag cgccgccgtg ctcggtttac tcggcctggt gcctggaatg ccgaacctgg
     1861 tatttttgct gttcactgcc ggattgctcg ggctggcctg gtggatacgc ggacgcgaac
     1921 aaaaagcgcc tgccgaaccc aaaccggtaa aaatggcaga gaataatacc gttgtcgaag
     1981 cgacgtggaa cgatgtacaa ctggaagatt ctctgggaat ggaagtgggt tatcgactga
     2041 tcccgatggt cgatttccag caggatggtg agttgttggg ccgtatacgc agtatccgca
     2101 agaaatttgc ccaggagatg ggatttctgc cgccagtggt gcacattcgc gacaatatgg
     2161 atctgcaacc tgcccgctat cgcattttga tgaaaggcgt ggagattggc agtggtgatg
     2221 cttatccggg gcgctggctg gcgattaacc ctggaaccgc tgccgggacg ttacctggtg
     2281 aggcgaccgt cgatccggca tttggcctga atgctatctg gattgaaagt gcgctaaaag
     2341 aacaggcgca gattcagggg tacacagtgg ttgaggccag cacggtggta gcaacgcatc
     2401 ttaaccacct cattagccag catgccgcag agctgtttgg tcgtcaggag gcgcaacagc
     2461 tgttggatcg cgtcgcccag gagatgccaa agctgacgga agatctcgtt cctggcgtcg
     2521 tcacgctcac cacactgcat aaagtgctgc aaaatctcct cgatgaaaaa gtaccgattc
     2581 gcgatatgcg caccattctc gaaacgctgg cggaacatgc gcccatccaa agcgatccac
     2641 atgaattaac cgccgtcgtg cgcgtggcgt tgggacgggc gattacccag cagtggtttc
     2701 ctggcaaaga tgaagtccat gttattggcc tcgatacacc gctggaacgt ttgttactac
     2761 aggcgctgca gggcggggga ggactggagc cagggctggc ggatcgttta ctggcgcaaa
     2821 ctcaggaagc gctatcccgt caggagatgc tgggcgcgcc gccagtattg ttggtgaacc
     2881 acgcgctgcg accattattg tctcgcttcc tgcgccgcag cttgccgcag ttagtggtcc
     2941 tgtcgaatct ggaactgtct gataaccgac atatccgcat gacggcgaca attggcggca
     3001 aataatgaga accttattag caatattatt gtttccgctg ctggtgcaag ccgccgggga
     3061 ggggatgtgg caggcaagta gtgtaggaat tacgctaaat catcgcggtg agtcgatgtc
     3121 gtctgcgcct ctttctacgc gacaacctgc ttcaggattg atgacgctgg tagcgtggcg
     3181 ttatcagctt atcggcccga caccttcagg actgcgggtt cgcttgtgtt cgcaatctcg
     3241 ttgtgtcgaa ttagaggggc agagcggaac caccgtggcc ttttccggta tagcggcagc
     3301 agaaccgttg cgatttatct gggaagtgcc aggcggtggg cggttaattc caccgctaaa
     3361 ggtacaacgt aatgaagtga ttgtgaatta tcgctgaagg acaaaaaaat tacgcgctcg
     3421 cgagtatgtt taataactca gtgaaaaagg ctcgatg
//
LOCUS       DAKISA010000232         3322 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.235, whole genome shotgun sequence.
ACCESSION   DAKISA010000232 DAKISA010000000
VERSION     DAKISA010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.235"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..320)
                     /gene="fliZ"
                     /locus_tag="OVW45_004411"
     CDS             complement(<1..320)
                     /gene="fliZ"
                     /locus_tag="OVW45_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCV5528683.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTV"
     gene            complement(366..1085)
                     /gene="fliA"
                     /locus_tag="OVW45_004412"
     CDS             complement(366..1085)
                     /gene="fliA"
                     /locus_tag="OVW45_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HCV5528684.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(1250..2824)
                     /locus_tag="OVW45_004413"
     CDS             complement(1250..2824)
                     /locus_tag="OVW45_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCV5528685.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALSEINNNLQRIRELSVQATNG
                     TNSDSDLNSIQDEITQRLSEIDRVSNQTQFNGVKVLASDQTMKIQVGANDGETIEIAL
                     DKIDAKTLGLDNFSVALGKVPMSSAVALKSEAAPDLTKVNATDGSVGGAKAFGSNYKN
                     ADVETYFGTGNVQDTKDTTDATGTAGTKVYQVQVEGQTYFVGQDNNTNTNGFTLLKQN
                     STGYEKVQVGGKDVQLANFGGRVTAFVEDNGSATSVDLAAGKMGKALAYNDAPMSVYF
                     GGKNLDVHQVQDTQGNPVPNSFAAKTSDGTYIAVNVDAATGNTSVITDPNGKAVEWAV
                     KNDGSAQAIMREDDKVYTANITNKTATKGAELSASDLKALATTNPLSKLDEALAKVDK
                     LRSSLGAVQNRFDSAITNLGNTVNDLSSARSRIEDADYATEVSNMSRAQILQQAGTSV
                     LAQANQTTQNVLSLLR"
     gene            3079..3322
                     /locus_tag="OVW45_004414"
                     /pseudo
     CDS             3079..3322
                     /locus_tag="OVW45_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460913.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
BASE COUNT          852 a          789 c          713 g          968 t
ORIGIN      
        1 accgtacctg ggctcatttc agtttgctca aaaagaaatt gcttaaagcc gataatatcg
       61 aaaaaatcac tctgcgtttt gcagtgtaaa tcaccacaaa atcggcacaa tgccgcccat
      121 gatttttgtt ccacttgcca gccattttca tcaagcagca tgtccaggcg ggaaatctcg
      181 attttattca ctattttgcc gtcgcgaacg agggtaatgc ggtcgagtaa tttacggcaa
      241 tgcgcgcaat gagtctggct gtgtttaaag tctttaagat agcggcttaa tggccgtctt
      301 ttcaggtgct gcaccatcat taagaactcc tggtagtcaa aattaaagtg cggcatttac
      361 tgacgttata acttacccag tttagtgcgt aaccgtttaa tggcctggct gtgtaactga
      421 ctgacccgcg attccccgac ctccagcacc gcgccaatct ctttgagatt cagctcttcc
      481 tggtaataga gggttaatac cagtttttcg cgctccggca acgtttcgat ggcttccatc
      541 acccgctggc gcagattact gtccagcagt tgttgtagcg ggttttctcg ctgatgatca
      601 tcagtaacca gttcgatgct atcgccgtgc tcttcgcgcc actcatcgta ggagaagagc
      661 tggctgttat tggtgtcgag caacatttgg cgataatcgg caatatcgat ccctaaacgt
      721 tccgctacct cagtttccgt ggcgttgcgg ccaagttcct gctccagttg ccctattgcc
      781 tgtgccactt cacgcgcgtt gcgtcgcacg ctgcgcggca cccagtcacg gctgcgaagt
      841 tcatccagca tagcgccacg gatacgctgc actgcgtaag ttgtaaatgc cgttccttgt
      901 agggcgtcat agcgttcgac ggcattaagt aacccaatgc cgcccgcctg tagcagatcg
      961 tcaagttcca cgctcgcggg cagtcgaacc tgcaggcgca atgcttcgtg acgcaccagc
     1021 gggacataac gctgccacag cgagtgttta tccattacac cttcagcggt atagagtgaa
     1081 ttcacgataa acagccctgc gttatatgag ttatcggcat gattatccgt ttctgcaggt
     1141 tttttaatcg gacgattagt gggtgaaatg aggggttatt taggggttac cggtaaattg
     1201 cggacagaaa aaaccccgcc ggtggcgggg tttgagcgat aagtgtaaat taacgcagca
     1261 gagacagtac gttctgcgtg gtctggttag cctgtgccag aacagaggta cccgcttgtt
     1321 gcaggatctg cgcacgagac atgttagaca cttcggttgc gtagtcagca tcttctatac
     1381 ggctacgggc agaagacagg tcgtttacgg tgttgccaag gttggtgatg gcagagtcga
     1441 aacggttttg tactgcaccc aaagaactgc gcaacttatc aacttttgcc aaagcttcgt
     1501 ctaatttgga taatggattt gtggttgcta aggctttcaa atctgaggca ctgagttcag
     1561 cacctttggt tgccgtctta ttcgtgatat tggctgtata aaccttatca tcttcacgca
     1621 taattgcctg tgcagaacca tcatttttta ctgcccattc aactgcctta ccattaggat
     1681 cagtaataac agacgtgtta cctgtagcgg catctacatt tactgcaatg taggtgccgt
     1741 ctgatgtttt agcagcaaat gaattaggta caggattccc ttgggtatct tgtacttggt
     1801 ggacatctag gttttttccc ccaaaataaa cagacattgg tgcatcatta taagctaatg
     1861 ctttacccat tttacccgca gctaaatcaa ctgatgtggc agaaccatta tcttcaacaa
     1921 atgcagttac acgaccacca aagtttgcta actgaacatc cttaccaccc acctgaactt
     1981 tttcataacc tgtagagttt tgtttcaata atgtaaaacc gttcgtgttg gtattattat
     2041 cttgaccaac aaaataagtc tgcccttcca cctgtacttg ataaactttt gttcctgcag
     2101 taccggtcgc atcagttgta tcctttgtat cttgtacatt accggtacca aaataagttt
     2161 caacatcagc atttttataa ttgctaccga atgctttagc acctcccaca ctaccatcag
     2221 ttgcatttac cttagttaag tcaggagcgg cttcgctctt aagtgcaacc gcagaggaca
     2281 ttggaacttt tcctagtgct acgctaaagt tatcaagccc caaggtttta gcatcaattt
     2341 tatcaagggc aatctcaatg gtttcaccat cgttcgcacc tacttgaatt ttcatagtct
     2401 gatcagaagc cagaactttt acaccattaa attgtgtctg gttagaaaca cgatcaattt
     2461 cactaaggcg ttgtgtaatt tcatcctgaa ttgaattcag gtcggagtca gagtttgtac
     2521 cattagtggc ctgtactgac aattcacgaa tacgctgtaa gttattgttg atttcagaaa
     2581 gcgcaccttc agtggtctgc gcaacagaaa taccatcatt cgcgttacgg gaagcctgag
     2641 tcagaccttt aatatttgct gtaaaacggt tagcaatcgc ctgacctgct gcgtcatctt
     2701 tagcactgtt aatacgcagg ccagaagaga gacgttcaat ggcggagctc agagaagact
     2761 gagatttgtt caggttattc tgggtcaaca gcgacaggct gtttgtatta atgacttgtg
     2821 ccatgatctt ttccttatca attacaactt gatgttattg ggctgttgcc cacggtttct
     2881 caccgtaccc cttgtatcgg cacctgaatt tcgaacttta gaaaatttta gtttctctcc
     2941 tgttttttct taacggctga aatatccgta atatacaact ctactctgcg catcatcttc
     3001 gtgaatcaac tattaaactt tgcgcacaaa ttgccgataa cccgcttatc tactgttttc
     3061 aatcaaaagg aattaggtat ggcttcattt acatctcttg gcgttggttc gaatctgccg
     3121 ctggatacat tgctaaacaa cctgaccatc gctgaaaaaa aacgcttaaa cccaattact
     3181 cagcaacaga gtgacaatac cgcacgtctg acagcctatg gcacattaaa aatgtgcatt
     3241 agaaaaattc cagactgcaa atactgcgtt aaataaagcc gatctgttca aaagcactac
     3301 cgttaccagt agcaatgaag ac
//
LOCUS       DAKISA010000233         3239 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.236, whole genome shotgun sequence.
ACCESSION   DAKISA010000233 DAKISA010000000
VERSION     DAKISA010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.236"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(79..606)
                     /gene="mobB"
                     /locus_tag="OVW45_004415"
     CDS             complement(79..606)
                     /gene="mobB"
                     /locus_tag="OVW45_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCV5528686.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTEKPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(588..1172)
                     /gene="mobA"
                     /locus_tag="OVW45_004416"
     CDS             complement(588..1172)
                     /gene="mobA"
                     /locus_tag="OVW45_004416"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCV5528687.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHVLASRLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            1242..1511
                     /locus_tag="OVW45_004417"
     CDS             1242..1511
                     /locus_tag="OVW45_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCV5528688.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1588..2574
                     /gene="srkA"
                     /locus_tag="OVW45_004418"
     CDS             1588..2574
                     /gene="srkA"
                     /locus_tag="OVW45_004418"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCV5528689.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQTEAVGRYLGRMHQTGHKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFGEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2591..3217
                     /gene="dsbA"
                     /locus_tag="OVW45_004419"
     CDS             2591..3217
                     /gene="dsbA"
                     /locus_tag="OVW45_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCV5528690.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          829 a          779 c          786 g          845 t
ORIGIN      
        1 tcaggatggc ctttttgcat tggcgcagaa aaaaatgccg gatgcgacgc tggcgcgtct
       61 tatccagcct actcttgctt atccgttctg cttttgcatc cactccacca caaaatcagc
      121 cagcccctca acatcattaa tatccagtaa tgcgacatca agattaagcg gcacatcact
      181 ggctacagca ataacatgcc tgtctatcac taattcttca ggtcgatgtc cggctccatc
      241 gcgaaacagc acaatctttg cgatctcttc atgcttaaac ccttcgacca gaatcaaatc
      301 cagctttgag gtatccattc gacttgcgag aaactgtaga tccagctctt cttcgtctgg
      361 tttttccgtc atcaaggccc atcgttgctg gctggcaacg atggtttgtg ccgcgccagc
      421 cttgcgcagc tcatagctat ctttgcctgg cttatcaaca tccatatcat ggtgcgtatg
      481 cttaatcagc cctggacgga tccctctggc gcataatgcc gggatcagtt ttttcaaaag
      541 cgtagttttt ccggtaccac tccacgcggc aaaggcgagt aacggtatca tcgtttttcc
      601 tgccatcggg caagctcctc tggcgtattc acgttaacaa atgcatcttt atgatcgctg
      661 aaatcaaccg catggccgcc agccagacgc ataaatgcca ttacccggcg ttctcctgct
      721 tgcagatatt ctagtaataa aggctcaata gcacggttta ccagagcaat agtcgggtga
      781 tcgcgttcac cgtcatggac ccacacgaca ggcgcatctt tgcgctgatg attaagccgg
      841 gaagctaaaa catggggaat gtaaggcgta tcgcacggac aaaacaaaaa ccactcacca
      901 gcttcctgct gcattactga aagcattcct gccagagggc ctgggtaatc cgccagtgaa
      961 tcttcaatca ctttcagacc gcttgcctga tagatttcct gatgacgatt ggcattaacc
     1021 acgacgtgag agagctgcgt cataagcgcg tcagcgacat gttgccataa tggtttgccg
     1081 tttaattcaa gcaatccttt atctacgccg cccattcgtc tggctttacc gcctgccagc
     1141 acaacgcctg ttatcgtcgt catcagattc accgatatcg cctcttttat tgtgggattg
     1201 accctgctaa cgtgtctgtc tcaagagtaa ggagcatcac tatgaaatgt aaacgtctga
     1261 atgaagttat tgaactcctc cagccagcct ggcaaaaaga gccagacctt aacctgttgc
     1321 aatttttgca gaaactggcg aaagagtcag gttttgacgg cgaactggcg gatttgacgg
     1381 atgacattct gatctatcac ctgaaaatgc gtgattccgc gaaagatgcg gtgatcccgg
     1441 gtttgcagaa agattatgaa gaagacttca aaacggcgct gttacgcgct cgcggcgtaa
     1501 ttaaagagta aaagcttgta agcggcgcca ccaaaatcat cgtgaaatga tatccttcat
     1561 cattcgtaat gttttccgga tgatgggatg aacaacagcg cttttacttt ccagacacta
     1621 cacccggata ccatcatgga cgctctgttt gagcaaggga tccgggtgga ttccggtctt
     1681 accccgctta acagctatga aaaccgtgtc tatcaatttc aggacgaaga gcgtcgacgt
     1741 tttgtcgtca aattttatcg ccctgaacgt tggacagccg atcaaatcct cgaagaacat
     1801 caatttgcgt tgcagctggt aaatgatgaa gttccggtcg cagcacctgt ggcctttaac
     1861 ggtcagactt tattgaatca tcagggattt tatttcgctg tttttccaag cgtcggtggt
     1921 cgccagttcg aagctgataa tatcgatcag acggaagcgg ttgggcgtta tttagggcgt
     1981 atgcaccaga cggggcacaa acagcttttt atccatcgcc cgaccatcgg tctgaatgaa
     2041 tatctcattg agccacgcaa gctgtttgag gacgctacac tgataccttc cgggttgaaa
     2101 gcggcattcc tgaaagcgac agatgagctg atcgccgccg ttacagcaca ctggcgggaa
     2161 gatttcaccg ttctgcggct acatggagac tgccacgccg ggaatattct ctggcgcgat
     2221 ggtccaatgt ttgttgatct ggatgatgca cgtaatggtc cagctattca ggatttgtgg
     2281 atgttgctca atggcgataa agccgagcag cggatgcaac tggaaactat tattgaagct
     2341 tatgaagaat ttggcgagtt cgacaccgct gaaatcggac tgattgaacc tttacgcgcc
     2401 atgcgtttgg tttattatct tgcctggcta atgcggcgtt gggctgatcc cgcgttcccg
     2461 aaaaatttcc cgtggttaac cggggaagat tactggctgc gacaaacggc gacttttata
     2521 gaacaggcaa aagttctaca agaaccccct ttacaattaa cacctatgta ttaatcggag
     2581 agagtagatc atgaaaaaga tttggctggc gctggctggt ttagttttag cgtttagcgc
     2641 atcggcggcg cagtatgaag atggtaaaca gtacactacc ctggaaaaac cggtagctgg
     2701 cgcgccgcaa gtgctggagt ttttctcttt cttctgtccg cactgctatc agtttgaaga
     2761 agttctgcat atttctgata atgtgaagaa aaaactgccg gaaggcgtga agatgactaa
     2821 ataccacgtc aacttcatgg gtggtgacct gggcaaagat ctgactcagg catgggctgt
     2881 ggcgatggcg ctgggcgtgg aagacaaagt cactgttccg ctgtttgaag gcgtacagaa
     2941 aacccagacc attcgttcag catctgatat ccgcgatgta tttatcaacg caggtattaa
     3001 aggtgaagag tacgacgcgg cgtggaacag cttcgtggtc aaatctctgg tcgctcagca
     3061 ggaaaaagct gcagctgacg tgcaattgcg tggcgttccg gcgatgtttg ttaacggtaa
     3121 atatcagctg aatccgcagg gtatggatac cagcaatatg gatgtttttg ttcagcagta
     3181 tgctgataca gtgaaatatc tgtccgagaa aaaataattc aatgtaaatt acataaggc
//
LOCUS       DAKISA010000234         3219 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.237, whole genome shotgun sequence.
ACCESSION   DAKISA010000234 DAKISA010000000
VERSION     DAKISA010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.237"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            7..510
                     /locus_tag="OVW45_004420"
     CDS             7..510
                     /locus_tag="OVW45_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCV5528691.1"
                     /translation="MNNIAPQSPVMRRLTLLDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(556..972)
                     /gene="rraB"
                     /locus_tag="OVW45_004421"
     CDS             complement(556..972)
                     /gene="rraB"
                     /locus_tag="OVW45_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCV5528692.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1134..2138
                     /gene="argF"
                     /locus_tag="OVW45_004422"
     CDS             1134..2138
                     /gene="argF"
                     /locus_tag="OVW45_004422"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCV5528693.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKTFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            complement(2210..>3219)
                     /locus_tag="OVW45_004423"
     CDS             complement(2210..>3219)
                     /locus_tag="OVW45_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCV5528694.1"
                     /translation="LLEKPLNNALLAIRKEHLLLMPYVCNESISYLLGEKGILKEIDD
                     LNAVNNYLLNNKKATDNEINDIKVNLSHILIDSLDDAKVNLTPVIDSILETFLKSPYI
                     NDVRILDWCFNKRMQYFGDSEKIKYACSVINHIDFSCDQSKDFSCDQSKIKIAETLFF
                     NLDKEPYKNSRKLQELIWDKLVAYVNDFSLSNQEKSRLILRLFDDVKLLFNEVPVSIL
                     VNDIFLKDFFMKQPDFAKWYFYQLLKCYEGEQLYLNELGYVYGDEEKTKEIVNKLPGY
                     VVKIFEEKMGNELKIRTRMMKILRNGKINIYGYINEEQLGKLNPPEDLRIAIKKLGWN
                     N"
BASE COUNT          893 a          719 c          675 g          932 t
ORIGIN      
        1 taaactatga ataacattgc gccgcaatca cctgtaatgc gtcgcctgac gctgctggat
       61 aatcctgcta tcgcccgcgt cattcgtcag gtatccgccg aatacggtct taccgctgat
      121 aaaggctaca ccgtcgccga tccgaatctt gacgagctgt atcaagtgta tagtcagcct
      181 ggccatgcat attgggtggt tgagtacgag ggtgaagtgg tcggcggcgg tgggattgcg
      241 ccattaaccg ggagtgagtc ggacatttgc gaactgcaaa agatgtattt tcttccggct
      301 attcgcggta aagggctagc gaaaaaactg gccttaatgg cgatggagca ggcacgagag
      361 atgggcttca aacgctgcta tctggaaacg accgcttttt taaaggaagc cattgcgctt
      421 tatgagcatt tgggctttga gcatatcgac tatgcgcttg gctgcacggg ccatgtcgat
      481 tgtgaagtgc ggatgctacg cgaactctaa aaaaatgccc gctagtatgt gctatcgggc
      541 attttaatca attaattagt ggcgaacccc gtcatcgtct tcatcgacaa aatcttcatc
      601 gtcgccatct tcgccgttag gatcttcaaa gtaagtgccc caaccgtcgt actcaacgtc
      661 aaatttctcc gccagcgtca tcagttgttc aacctgggca tcgatcagat cggcattcaa
      721 cgcgcactcg ctgaggatgt cgcagcaaat cacgatatca ccatcttcaa cttccagctc
      781 ttctggatcg gtcacttcgt aaccgagttt aaacgcttca actgccgctt tttccagagt
      841 ttccagatcg tctgcggaaa gatggtgttc gatggtgtac aacgcgtccg gatcgctgcc
      901 atcttccagt aattcttcaa taatcaaacg tgtttcttca cgctgttctt ccagttgttc
      961 cgggtttgcc atggctcatt cctcttaatg tcctgccgat actcttattg tcacacacca
     1021 ctgacattgc ctccaccttt gtgataaaga tttatgcttt agacttgcaa atgaataatc
     1081 atccatataa attgaatttt aattcattaa ggcgttagcc acaggaggga cctatgtccg
     1141 ggttttatca taagcatttc ctgaaattac tcgatttcac gccagctgaa ctcaacagcc
     1201 tgctgcagtt agccgcgaag ctgaaagccg ataagaaaag cggtaaagaa gaagccaaac
     1261 tcactggtaa aaacatcgcg ctcatcttcg aaaaagactc gacccgtacc cgatgctctt
     1321 tcgaagttgc cgcatatgac cagggggctc gtgttactta tctcggccca agcggcagcc
     1381 aaattggtca taaagagtcg attaaagaca ctgcccgcgt gcttggtcgc atgtatgacg
     1441 gtattcagta tcgcggctat ggtcaggaga ttgtcgaaac actggcggaa tacgctggcg
     1501 tgccggtatg gaacggcctg accaatgagt ttcaccccac gcagctgctg gcggatctcc
     1561 tcaccatgca ggagcatttg cccggcaaaa cgttcaacga aatgacgcta gtctatgcag
     1621 gtgacgcacg taacaacatg ggcaattcga tgctcgaagc tgcagcgctt accggtctgg
     1681 atttgcgtct ggtcgcgcca caggcatgct ggccggaagc tgcgctggtt acggaatgcc
     1741 gcgccctggc acaacaaaat ggcgggaata ttacgctgac ggaagatgta gctaaaggag
     1801 ttgaaggtgc tgactttatc tataccgatg tatgggtgtc gatgggtgaa gcaaaagaga
     1861 aatgggctga acgcattgca ttgctgcgtg attatcaagt gaacagcaag atgatgcagt
     1921 tgaccggtaa cccggaggtc aaattcctcc actgcctgcc cgcgtttcat gacgaccaaa
     1981 cgacgcttgg caaaaaaatg gcggaagagt ttggcctaca tggcggaatg gaagtgactg
     2041 atgaggtctt cgaatctgcc gccagcattg tatttgatca ggcggaaaac cgcatgcata
     2101 ccatcaaagc ggtgatggtc gcgacgctca gtaaataatt gatatcgcta taaagatgcc
     2161 gggaaataca aaaacatccc ggcgtcacat tattttatgc agtgatagtt caattattcc
     2221 accccaattt ttttattgct atacgtaaat cttccggagg atttaatttt cctaactgtt
     2281 cttcattaat atatccatat atattgattt tgccattccg gaggattttc atcattcttg
     2341 ttctaatttt taattcatta cccatctttt cttcaaaaat cttaacgaca taacccggaa
     2401 gtttatttac aatttctttt gttttttctt catcaccata aacatatcct aactcattaa
     2461 gatacagctg ttcgccctca taacacttaa gtaattgata gaagtaccac ttagcaaaat
     2521 caggttgctt catgaaaaaa tccttcaaaa aaatatcatt tactaaaatg ctgacaggca
     2581 cttcattaaa tagtaactta acatcatcaa ataaccttag tattaagcgt gatttttctt
     2641 gattgcttaa gctaaaatca ttgacatatg caaccaactt atcccaaatc aattcctgta
     2701 atttacggct atttttatag ggttctttat cgagattgaa aaataaggtc tcagctattt
     2761 taattttaga ttgatcgcag ctaaaatctt tagattgatc gcagctaaaa tcaatatgat
     2821 ttatcacgga gcatgcatac tttatctttt cagaatcacc aaaatattgc attcttttat
     2881 taaaacacca atccagtatt cttacatcat taatatatgg ggattttaaa aaagtctcca
     2941 gaatcgaatc gatgaccggt gtaaggttaa cttttgcatc atctaaggaa tcaataagaa
     3001 tatgagacag gttaactttg atgtcattaa tttcattgtc agtagctttc ttattattaa
     3061 gaagataatt atttactgcg ttaaggtcat ctatttcttt cagtatccct ttttcaccaa
     3121 gcaagtatga aatcgattca ttacatacat aaggcattaa tagcagatgt tctttcctta
     3181 ttgctaataa tgcattgttc aatggttttt ctaataaat
//
LOCUS       DAKISA010000235         3214 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.238, whole genome shotgun sequence.
ACCESSION   DAKISA010000235 DAKISA010000000
VERSION     DAKISA010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.238"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..493
                     /locus_tag="OVW45_004424"
     CDS             <1..493
                     /locus_tag="OVW45_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCV5528695.1"
                     /translation="RAPFIITPPLFRIEAGDDSSLRIIKTADNLPENKESLFYINVRA
                     IPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRSDHSLNIYNPT
                     EYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAINDYGGSSGTETR
                     PLQ"
     gene            523..3039
                     /locus_tag="OVW45_004425"
     CDS             523..3039
                     /locus_tag="OVW45_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCV5528696.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQTSSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPKFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWNYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLNGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHTHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3050..>3214
                     /locus_tag="OVW45_004426"
     CDS             3050..>3214
                     /locus_tag="OVW45_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCV5528697.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGRE"
BASE COUNT          938 a          771 c          729 g          776 t
ORIGIN      
        1 ccgtgcccca tttattataa ccccaccgct atttcgtatt gaagctggcg atgactcatc
       61 actgcgaatt attaaaacag ctgataacct gcctgaaaat aaagagtcgc tgttctacat
      121 taatgttcgt gccattccag caaagaaaaa atcagatgat gttaatgcta acgagttgac
      181 gctggtattt aaaacacgga tcaaaatgtt ttatcgcccc gcacacctga agggacgggt
      241 aaacgatgcg tggacatcac tggaatttaa acgtagtgac cattcactca atatatataa
      301 cccaactgaa tattacgtcg tatttgccgg actggcagtc gataaaaccg atctcacaag
      361 caaaattgaa tatatcgcgc ccggagaaca taaacagtta ccacttcctg catctggcgg
      421 aaagaacgtg aaatgggctg caatcaatga ttatggcggc agttccggga cagaaactcg
      481 tccactgcaa taacaaatat aaaaaacaca ggtcatcagg gaatgccaca acgacaccac
      541 cagggacata aacgcacacc gaaacagttg gcgctcatca tcaaacgttg tttgccgatg
      601 gtgctcactg gcagcggcat gctttgcact accgctaacg ccgaagagta ttatttcgac
      661 cccattatgc tggaaactac aaaaagtggt atgcaaacaa ccgatctgtc acgtttttca
      721 aaaaaatacg cacaactacc aggaacttat caggttgata tctggctgaa taaaaagaag
      781 gtttcacaga aaaaaattac atttaccgcc aatgcagagc aacttctgca gccacagttt
      841 acggtagaac aactacgtga gctgggtatt aaggtggatg aaatcccggc gctggctgaa
      901 aaagatgacg atagcgtgat caactcgctt gaacaaatca ttcccggtac agcagctgaa
      961 tttgatttca atcatcagcg acttaatttg agcattcccc aaattgcact gtaccgtgat
     1021 gcaagaggtt acgtctcccc ttctcgttgg gacgatggta taccaacgct gtttaccaac
     1081 tactcgttta caggttctga taaccgttac cgccagggca atcgtagcca acgacagtac
     1141 ctgaatatgc aaaatggtgc taattttggc ccctggcgat tacgcaacta ttccacatgg
     1201 acacgcaacg atcagacatc aagctggaat accatcagta gttatttaca acgtgatatc
     1261 aaggcattga agtctcagtt gcttctggga gaaagcgcca ccagcggcag tattttttcc
     1321 agctacacct ttactggcgt tcaactcgct tccgacgata atatgttgcc aaacagccag
     1381 cgcggatttg ccccaacggt acgcggtatc gcaaacagta gtgcaatcgt gactatcagg
     1441 caaaatggtt atgtgatcta tcaaagcaac gtgccagcgg gtgcctttga aattaacgat
     1501 ctctacccct cttccaacag cggcgattta gaagtcacga ttgaagaaag tgacggtacg
     1561 caacgtcgct ttatccagcc ttattcttca ttacccatga tgcagcgacc tgggcatcta
     1621 aaatatagcg cgaccgctgg acgctatcgc gctgatgcaa acagtgatag caaagaaccc
     1681 aaatttgctg aagccacggc aatatatggt ttgaataata cttttacgct gtatggtggc
     1741 ctgctcggtt ctgaagatta ttatgcgctg gggatcggta tcggcggcac acttggcgca
     1801 ctgggcgcgt tgtcgatgga tatcaacaga gctgacaccc aattcgataa ccagcactct
     1861 tttcatggct atcaatggcg tacgcagtac atcaaagata tcccggaaac caacaccaat
     1921 atcgctgtca gctactatcg ctataccaac gatggctatt ttagttttga tgaagccaat
     1981 acccgtaatt ggaactataa cagtcgccaa aaaagtgaaa ttcaattcaa catcagccag
     2041 acaatatttg atggggtaag tctgtatgcc tccggttcgc agcaagacta ttggggcaat
     2101 aacgataaaa acaggaatat ctcagttggg gtttccggtc agcaatgggg aattggttac
     2161 agcctgaatt atcaatacag tcgctacacg gatcaaaata atgaccgcgc actctctttg
     2221 aatctcagta ttccgttaga acgctggtta ccgcgtagcc gggtttccta tcagatgacc
     2281 agccagaaag atcgcccaac ccaacatgaa atgcgtctta atggctcact gctggatgat
     2341 ggtcgcctga gctatagcct ggaacaaagt ctggatgacg ataacaacca taacagtagc
     2401 ctgaacgcca gttaccgttc accttatggc accttcagtg ccggatacag ctacggtaat
     2461 gacagcagcc aatacaatta cggcgttacc ggcggcgtgg ttatccatac tcatggcgtg
     2521 acgctctcgc aatatctggg caacgctttt gcgcttatcg atgctaatgg ggcttctggc
     2581 gtgaggatac aaaactatcc ggggattgct actgatccct ttggctatgc agtggttcct
     2641 tatctcacga cttaccagga aaaccgtctc tcggtagata ctacgcagct gcccgataac
     2701 gtcgatcttg agcaaacaac acagtttgtg gtgcccaaca gaggtgcaat ggtagcggcg
     2761 cgtttcaacg ccaatatcgg ttatcgcgta cttgttacag tcagcgatcg caacggtaaa
     2821 ccgttgccct ttggcgctct tgccagcaac gatgagacgg ggcaacaaag tatcgtcgat
     2881 gagggcggca tactatatct ctctgggata tcgagtaaat cacaaagctg gactgtacgc
     2941 tggggaaatc aggcagatca acaatgtcag tttgctttta gtacaccgga ttcagaacca
     3001 acaacctctg tattacaagg cacggcgcag tgccattaag gataaaaaaa tgaaaagagc
     3061 gcctcttata acaggacttt tgttgatatc cacatcctgc gcttatgcct cctcaggagg
     3121 gtgtggagct gacagcacta gcggtgcgac aaattacagc agtgtggttg atgatgttac
     3181 ggtgaaccag acagataacg tgacaggacg ggag
//
LOCUS       DAKISA010000236         3160 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.239, whole genome shotgun sequence.
ACCESSION   DAKISA010000236 DAKISA010000000
VERSION     DAKISA010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.239"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            107..493
                     /locus_tag="OVW45_004427"
     CDS             107..493
                     /locus_tag="OVW45_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCV5528698.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            502..831
                     /locus_tag="OVW45_004428"
     CDS             502..831
                     /locus_tag="OVW45_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCV5528699.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            803..3160
                     /locus_tag="OVW45_004429"
                     /pseudo
     CDS             803..3160
                     /locus_tag="OVW45_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
BASE COUNT          794 a          722 c         1037 g          607 t
ORIGIN      
        1 gacggagagt ttgtggcagt ggctgcggtg accgtcagcg caccataaca ggacgatact
       61 catcattgcc ccggttatcc ggggcttttt gcatccggag aacatgatgt ttctgaaaca
      121 ggatacgttt aattatgaaa aacagtccgt ggtgctcagt gagctgtccg ggctgcagag
      181 aattgaatat ctgacgtttg ttcagcagcg aacggcaaag tttgatgcac aggagggaga
      241 actgccggag gctgaacgac agattgcttt tctgcgtatg ggaatggata tcaatgcctg
      301 gctggtttcc cgctcactgt ggaatgctga gcagtctcag gatgttgaga cgctttgcgc
      361 atccattatg acaacatggt cgtatgatgc gctgggcgcg ggggcggaga gggttctgtc
      421 gctgagcggt atggggacca ttgagaatgc cggggatgat gatcatgagg cgctgacgcc
      481 ggaaaagtcc tgacgcggga aatgcagttt gtcatgcggc ttgcccggga gttccggcgg
      541 gcagactggc ggcggatgct gtcggatatg tcggccactg agcttggtga gtggggcgat
      601 tatttccgga tgcagagctt cagtgatgtg tggatggatg cgcagtttgc ctcgctgaag
      661 gcattgatcg tgagaatggt gtccggcagc agcgatgctg cggtggctga tttcagcctt
      721 ttaccggaag agaacgggat accggagcga acggacgaag aactgatgca tcttggggaa
      781 ggtatttccg gaggtgtgcg ttatggacca gatagccaac ctggtcattg atttggggat
      841 tgatgcggca gagtttaaaa atgaaattcc ccgtatcaaa aaccttctga atggtgcagc
      901 cagcgatgca gaacggtctt ctgcccgtat gcagcgtttt atggagcgtc agactcaggc
      961 ggcccggcag acaatgcagg cggcttcttc ggctgcaaca gcagcatcag cccatgcgca
     1021 gacggtggaa aagaacgcac gggctcatga acgcatggcc cgtgaggtgg aacaaacccg
     1081 tctgcgcgtg gatgccctga atcagaaaat gctcgaggaa caggcgcagg ccagggcact
     1141 ggcggaggcg caggataaag cggctgccgc cttttatcgc cagattgaca gtgtgaaaca
     1201 ggccggtgcg gggcttcagg aattacagcg tattcagcag cagatccgac aggccagaaa
     1261 cagtggcggg gttggtcagc aggattatct ggcgctgatt tcggagatca cggcgaaaac
     1321 ccgtgccctg acgcaggcag aggaacaggc cacccggcag aaagcggcgt ttatccgcca
     1381 gcttaaagag caggcaaccc gccagaatct gtcgtcttct gagttgcttc gcgccagggc
     1441 ggctcagctg ggggtaagca gtgctgcaga agtgtatatc cgcaaaatgg agcgggcagg
     1501 aaaagccaca cattcgctgg ggctgaaaag tgcggcagcc cggcgggagc tgggggtgtt
     1561 aatcagtcag atggcgcgcg gcaattttgg tgcgctgagg ggatccggga taacgctggc
     1621 taaccgtgcc ggatggacag gcgcactgat gtcgccgaaa ggcatgatga ctggcggcgt
     1681 tattggcgga cttgtcgcgg cggtcctggg tctgggtaaa gcctggcatg acggccggaa
     1741 ggagggcgag gaatttaacc gtcagctggc gctgacggga cattatgccg gtgtcactgt
     1801 cgggcagttg tggaaactca gccgggccat atccgggaat ggtatcacgc aacatgcggc
     1861 agccggtgcg ctggcacagg tggtgggcag tggggcattt catggaaacg atattggtat
     1921 ggtggcgaaa gctgccgcac agatggagcg atcggttggc cagtcggtca gcgataccat
     1981 aaatcagttt aagcggctga aggatgatcc tgtaaatgcc gcgaaggcgc tggacaatgc
     2041 gctgcatttt ttgactgcca ctcagcttga gcagatacgc gtccttgggg agcaggggcg
     2101 gtccagtgat gcggcccgga tcgccatgtc tgcgctggca gaggaaaccg gtaaacgcac
     2161 atctgatatt gataataatc tcaatgcgct gggtagtacg ctgcaaacct tgtctgactg
     2221 gtggaagcag ttctgggatg cggccatgaa tattggtcgt gaagactcgc tggatgcgca
     2281 gattgatgcg ttacaggaaa aaattcagcg cgcgaaaaaa tatccgtgga caaacgcctc
     2341 cacacaggtg gagtacgatc agcagcgtct taacgatctt caggagaaaa aacgccggaa
     2401 ggatttgcag gatgcaaaag cgcaggcaga acggaattac caggagcaac agaaacgccg
     2461 gaatgctgaa aatgccgcgc tgaaccggat gaatgaaacg gaagctgcac gacatcagcg
     2521 tgaaaatgaa cgttacgaaa aagccattaa gaaaaataca cgggcaaccc gtaatgatga
     2581 ggccacccgg ttattgctgc tgccagacag tcagcaggca ttgccactga aaggatgact
     2641 gaagcgcata aacagcttct ggccctgcag cagcgcatca gcgacctgga cgggaaaaag
     2701 ctgacggcag atgaaaagag tgtgctgacc cgtaagaatg agctgattca ggcgctgacg
     2761 ctgctggatg tgaaacagca ggagctgcag aaacagacag cgcttaacga cctgagaaaa
     2821 aaaacggttc agctgaccag ccagctggca gacaaagaac gtgcactgcg tgagcagcac
     2881 aatctggata ttgccactgc aggtatgggg gataagcagc ggcagcgcta ccaggcacag
     2941 ttgcgcatcc ggcaggaata ccggcaacag ttgcaacagc ttgagaatga cagtcgccag
     3001 aaaggcactt acgggacgga ggactaccgg agggctgagg aggtgctgaa ggggagcctg
     3061 aagcgacaac tgaatgaaaa caaacgctac tggcaggaac tggaagtggc gcagggcgac
     3121 tggaaaaacg gtgccatgcg ggcgtttcag cattttacgg
//
LOCUS       DAKISA010000237         3150 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.240, whole genome shotgun sequence.
ACCESSION   DAKISA010000237 DAKISA010000000
VERSION     DAKISA010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.240"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..315
                     /locus_tag="OVW45_004430"
     CDS             <1..315
                     /locus_tag="OVW45_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528700.1"
                     /translation="KTSKQDREITDVSVDSDHKNTQCPATDDELDPFDDEEDEREKAI
                     KRAEKKKRFELARQKNKVSGLDPFDDSEDDYYLSPKKKQTQDDKKQKIKPKMYFAGGG
                     IK"
     gene            315..605
                     /locus_tag="OVW45_004431"
     CDS             315..605
                     /locus_tag="OVW45_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528701.1"
                     /translation="MKEYHGEKRYKDYLLKRYSISREGHFMKDTHGEVYRIRPKKEGR
                     GYFFYDGVTDLKIDALRFAVLYHFDIWNSIHQLRLKDGDPSNLKATNIITKR"
     gene            926..1216
                     /locus_tag="OVW45_004432"
     CDS             926..1216
                     /locus_tag="OVW45_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528702.1"
                     /translation="MRHTVTRLLPEAYSRLAAIKDKYPAMSHTALVGEILKEHTALTP
                     FDAIKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADKLGV"
     gene            1216..1635
                     /locus_tag="OVW45_004433"
     CDS             1216..1635
                     /locus_tag="OVW45_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528703.1"
                     /translation="MLCTFDYHAFYRHRGHLVRENNGTWKDLTRNAEPFTHQQLKSRF
                     SRWKNAPADTGDFEIIHTFDYASEDDLHDAIKLIEDFNTSEDVDGDKYKSRGNRYNRD
                     DITQAINAGQSQRYITEKYGISRRQYFYVKSLINKDN"
     gene            1803..2126
                     /locus_tag="OVW45_004434"
     CDS             1803..2126
                     /locus_tag="OVW45_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528704.1"
                     /translation="MNNYPMQIMVDNDTAMMVQSFIDAGIAINFDKLLKLMADNAENI
                     SDFIQSVEFNEPRMMLPITDSNMKRLVIEQTNIYSISPEQFLKGAVTILYSDNILVTD
                     SVRVH"
     gene            2556..>3150
                     /locus_tag="OVW45_004435"
     CDS             2556..>3150
                     /locus_tag="OVW45_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528705.1"
                     /translation="MIRNEHEMLIEHRPSALDIHELTLRESFDRAWRESHEVNKGPYI
                     PPEPLEIPRVEIDFSMNERCELDLKLKLQRRYFQAIKDSQEAMRSVYEKIQSKLEDEI
                     DEMVISMELQTNSYAYFSRLDRDGWGYDPVEIGKVIADLPEGQRVVRTERAVRAPHRL
                     SLIVADKSQAELEEIAKEKLMKARENEINLLKSRLAKL"
BASE COUNT         1046 a          565 c          662 g          877 t
ORIGIN      
        1 aagacttcta aacaagacag agaaatcact gatgtgagtg ttgatagtga ccataaaaac
       61 acacagtgcc ccgccactga tgatgagttg gaccccttcg atgatgaaga ggatgaacgt
      121 gaaaaagcca ttaagagggc agagaagaaa aagagattcg agcttgcaag acaaaagaac
      181 aaagtatcgg gtttagaccc attcgatgac agtgaagatg attattatct ttcaccgaaa
      241 aagaaacaaa ctcaggatga caagaagcaa aaaattaaac cgaagatgta ttttgccggt
      301 ggaggaatta agtaatgaaa gaatatcacg gtgaaaagag atacaaagat tacctgttaa
      361 agcgttacag catttctcgt gaaggtcatt ttatgaagga tactcacggg gaagtttacc
      421 ggattagacc aaagaaagaa gggaggggtt atttctttta cgatggtgtg acagatttaa
      481 agattgacgc tttgaggttt gcagtgctgt atcattttga tatctggaat tcaatccacc
      541 agctacgatt aaaagacggt gaccccagca acttaaaggc caccaatatt atcacaaaac
      601 gttagtttgt tttaccatca tcaatcgtaa ttgtgatttc atatttgaaa agcttaatgc
      661 tgaaattgaa tcgtgtactt ttggcgataa tcatttttaa aagctgatac acaagaagca
      721 ccagaaacac aaagcaacct aagcttgcga atctgtgaga ttcagcaatt gtttcaagaa
      781 agcccattaa ttccccttat ttatcaaagg gaatgacctg tatcagcccc ctacaaccat
      841 attaacatgt aaatgagttg ttgacaatat gttataaata tgttaaagtt agttataagt
      901 caaagatttt aagaaggaaa atttaatgag acacacagtt acacgccttt tgccagaggc
      961 atattccaga ctggcagcaa tcaaagacaa atacccagcc atgagccaca cagcacttgt
     1021 tggtgagatt ctgaaggaac acacagccct tacaccgttt gacgctatca agggcgttgc
     1081 agagggttct aaggccatca gaatcacaac gggccaactg gaactactca aagcacagtc
     1141 cgcagtggca ggttgctcaa tgattcaggt gttgagtgcc aaggttcatc aactggcgga
     1201 taagctgggg gtttaatgct atgcacattt gattatcatg cgttttatcg ccatagaggt
     1261 caccttgtcc gtgagaataa cggaacatgg aaagatttaa ctcgtaatgc tgaacccttc
     1321 acacaccagc aattaaaatc ccgcttctcc agatggaaga atgcaccagc agatactggt
     1381 gattttgaga ttatccatac cttcgattat gcctcagaag atgaccttca cgatgcaatt
     1441 aagctgattg aagacttcaa tacctcagaa gatgtggatg gtgacaaata taaatcccgt
     1501 ggtaaccgct ataaccgtga tgatatcact caggccataa atgctggtca atctcagcga
     1561 tacattacag aaaaatatgg tatttcacgg agacagtatt tctacgttaa gtcattgatt
     1621 aataaagaca attaaaaata accatctaag aaacggtaga atgcacttta caggcagagt
     1681 tgcaccttaa tttgataaat taccttacca gccagtgcaa ctccctctgt aacccgcatg
     1741 gttactggcc tcagaatggc tattttaaga ggtctgaggt gtgtttcata ggagatttaa
     1801 aaatgaataa ttacccaatg caaattatgg ttgataacga cactgctatg atggtgcaaa
     1861 gcttcattga tgcaggtatc gcaattaatt ttgataaatt actaaagctg atggctgata
     1921 atgcagaaaa catctctgac tttattcagt cggtggagtt taacgagcct cgaatgatgc
     1981 taccaattac agacagcaac atgaaaagat tggttatcga gcaaacgaac atatacagta
     2041 tttccccaga gcaatttttg aaaggtgctg tgacaatcct gtacagtgat aatattctgg
     2101 tgacggattc tgtgagggtt cactaatggg tttaataatt gcttggacgt tgcttggctt
     2161 atttgttggt tgtattatta acaatgcggt aaataagaat tgatgttatc aacctgttaa
     2221 aaagctgttg acaaataacc ttaagcatgg cataatttta attatgggag agcaattctc
     2281 tcttattttt ctttcaataa tccgatggat ggatattctg agttggaaag gtgcaatgct
     2341 tttcgtcatg gcctttcagg ttagtctgag aggctatctc aaaaagtact cttgttcatt
     2401 cattattgaa tcctttgccc ttcacagaaa tgtttagggc tttttcagtt tttaaaagca
     2461 cacttggcag gttcttgttc agtcagactc ttcacctctg attgctgagt gtgcttctat
     2521 aaattgaaat aacaaataaa ctaaggggtg aacgaatgat tagaaatgaa catgaaatgc
     2581 ttatcgaaca cagaccaagt gcacttgata ttcacgaatt aactttgcgt gaatcttttg
     2641 atagagcttg gcgggaatca cacgaagtaa ataaaggtcc atatattcca ccagaaccac
     2701 tcgagattcc aagagttgaa attgattttt ctatgaacga acggtgtgaa cttgatttga
     2761 agttgaagct acaacgccga tatttccaag ctattaaaga ctctcaagaa gcaatgagaa
     2821 gtgtttacga gaaaattcag tccaagcttg aagacgaaat tgacgaaatg gttatttcga
     2881 tggagcttca aaccaattct tatgcttatt tctctcgact agaccgtgac ggttgggggt
     2941 atgaccctgt agagattggt aaagtgattg cagatttacc agaaggtcaa agagttgttc
     3001 gtacagaaag agctgtcaga gcaccacatc gactgtctct aattgttgct gataaatcac
     3061 aagctgagct tgaagaaatt gccaaagaga aactgatgaa agcaagagaa aatgagatta
     3121 atttgctgaa atcaagactg gctaagctga
//
LOCUS       DAKISA010000238         3098 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.241, whole genome shotgun sequence.
ACCESSION   DAKISA010000238 DAKISA010000000
VERSION     DAKISA010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.241"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..309)
                     /locus_tag="OVW45_004436"
     CDS             complement(<1..309)
                     /locus_tag="OVW45_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HCV5528706.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYSSRLFDPSVRLAADIRDNEG
                     R"
     gene            complement(306..692)
                     /gene="trbI"
                     /locus_tag="OVW45_004437"
     CDS             complement(306..692)
                     /gene="trbI"
                     /locus_tag="OVW45_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HCV5528707.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PMTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(689..1939)
                     /locus_tag="OVW45_004438"
                     /pseudo
     CDS             complement(689..1939)
                     /locus_tag="OVW45_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
     gene            complement(1941..2123)
                     /locus_tag="OVW45_004439"
                     /pseudo
     CDS             complement(1941..2123)
                     /locus_tag="OVW45_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2145..2456)
                     /gene="traL"
                     /locus_tag="OVW45_004440"
     CDS             complement(2145..2456)
                     /gene="traL"
                     /locus_tag="OVW45_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HCV5528708.1"
                     /translation="MLGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICVGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(2471..2821)
                     /gene="traA"
                     /locus_tag="OVW45_004441"
                     /pseudo
     CDS             complement(2471..2821)
                     /gene="traA"
                     /locus_tag="OVW45_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
     gene            complement(2867..>3098)
                     /locus_tag="OVW45_004442"
     CDS             complement(2867..>3098)
                     /locus_tag="OVW45_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCV5528709.1"
                     /translation="TGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWY
                     NNRRLLERLGHTPPAEAEKAYYASIGNDDLAA"
BASE COUNT          714 a          889 c          757 g          738 t
ORIGIN      
        1 ccgcccttcg ttatcccgga tatccgcagc cagtctgacg gacgggtcaa acaaccggct
       61 gctgtatttc tccgtgcgtc cgataccggg cacggcaggg ggccgcagac tgttgcggat
      121 cacccgctcc ttaaaggcat ccattttccg gcccatctca ccggactgct ccagtgccgt
      181 caggcgctgc atgatcaccg tcagcatgtc cggctccttt accggccaca gatcacccca
      241 ggtgccaaga tcggcggcgg ccacactctg cccccatatc agcagggcaa tcagcccccg
      301 gcatctcatg gttccgctct cattcgccgg gcaatatcct gctggatttc ccgggtgata
      361 tccggagccc cctgtaccac ggcaggcgac accagaatga ccgcgtggtg tttctgctgc
      421 cacgcctgca ggctggcttc cagtgccgtg ttaaaacgcg ctgaaagggc ttttgactgt
      481 gcttctgaca gttgtttctg actggcgctg tcaaaaaatg catccacagt ctgtttcatg
      541 ttgaaggcgg cagtcatcgg tgcgttcagt ctgacaatgc cgtaactgac ggctgcgttc
      601 agtaacacca ttgccaggca tcccggcacc gtccaccacc agtggctgcg tcggggggct
      661 gtaacatcag tcgttttttg cgttgtggtc atgcaacact cctgtatttc tcatgttctt
      721 ccagccaggc ttccagcgaa gccatctccg gccctgactt cttccacgcc agctgccaca
      781 ccgcctcatg aatactcagc ccctctttac gtttctgctg cacaaactca aaatccgggc
      841 cgtcagagct gtacatggcg cggcttaacg ggtccacaaa caggcggtgc caggaggaat
      901 ggttttccac ctgcagcagg aaggaactga accactggtc tttggccgca ccaaacttac
      961 caatcatgtc gcgctgcagt ggctggaact ggtccggata cagctggttg tattttgcga
     1021 actccttcgc actctgtttg aggataattt tgtaggagga gttaccccat gccgcacggg
     1081 ccgcactgga ggccttgtca gagtcaaagt cgacgatgtt ctgtgtgatg gtgatataag
     1141 caccggtatg acgacgggcg gtacggtaac ctttctcaat aaactcaccg accttgtgat
     1201 ttttgaagtc cagcagacgc cagccttcat caatgacgtt cagtttcttg aggttacgcg
     1261 gcgtgcggta catcctgttc tcgatgtaga taatcaggga gaacatcacc gcaaccagca
     1321 gtgacggacg gtcttccagt ccgcccagct ccagcaccac catttttgcg tcatcccgca
     1381 gggacggctc atcagagtta aaatactggc cgtaagtgcc gttggcagtg tactggtcaa
     1441 gcagcacaat catttcgtcc agacggctgc ggatagtcgg tgacccggca tactgctcgc
     1501 tgtcactggc gtttttcagg aaatccacca cgtcatcaat acgtgccctg ttctctttgg
     1561 ccagccagga ggccctgacc gcctgcagca gcagaccttc atgcacttca tccaggttac
     1621 cgttggggct ggccatcact gacaactggt cacggacacg ctccgctgac tggtcgatat
     1681 cggtgatgtt cgcaaacggg ttaaaacgca gggtttcacc gtccagatat acgccaccca
     1741 tgttctcaca cagggacttg tagccatccc ccatgtcgaa caccacggca aagcccccgg
     1801 agtccagcac gctgcggata agtggctgta tcagcccggt tttaccggcc ccggaggtgc
     1861 cacagaccgc catgttgtag ttggtgttat tcattccccg gaagaaaata tcgataaacg
     1921 ccagctggtt acggtaggtg tgacacggca aagggcacat taaatcccat tggggtcacg
     1981 gcagtgcgtt gctcattctg aagacggtag ttattcaccc cctgaatgag gttaacagag
     2041 aggctgagaa caataagcac tgacataaat ataaaggcga tggccattac acgactggta
     2101 cttaaacggg caccgtgttc catataacaa tcctggtatc agttctattt aatccactgc
     2161 cggaaacacg aatcgggaac attatgaaaa ataccgcgca gcagggctgt tggcatatac
     2221 cagtaaatca ggtcacgtaa ccaggaactg ccccgccctt ttttcagttt tttaatcccg
     2281 aaataaacca gaaccgctgc accaataccg aacagatatt tcgatgttgt gataccccag
     2341 ccaacacaga ttgctgcggg gatcagttca tccagtggca ggccaaacca gcggctctgg
     2401 ttggtcagtg tttccgggaa acgatatttc ttaagtttat tctcgtctcc caacatcgtt
     2461 ttatttcctg tcagaatccg gcaacagaca tacctactgc aataaataca gagagtatgg
     2521 caaagccgac caggaacttg acgtttttgg tcatcatgta catgactgca ccgaccagaa
     2581 cttcagccag aacaacccat ttaacgatac tggagtcctt accgaaggtc gccttaaccg
     2641 tttcctgacc tttagccatc aggtccgtgc ctttaccagc agccatagct aactcaggga
     2701 acaacatcat cagaacagca accgggatca ctgcacgaac caggcgaagc atattcagac
     2761 gagtgaattt agaaaaaaac gatttctttt tgacgggcgc agaagtaccc tgaacactta
     2821 acgctgaacc gccccgggtt tcctggagag tgttttatct gtgaactcag gctgccagat
     2881 catcgtttcc gatggaagca taataagctt tttctgcttc tgccggagga gtatggccca
     2941 gcctttccag caatcgtcga ttgttatacc agtccaccca cgtgagtgtg gccagttcca
     3001 cttctgcacg gtttttccag ctcttacggt gtattacctc cgctttgtaa agaccattga
     3061 tgctctccgc catcgcgttg tcatacgagt cgcctgta
//
LOCUS       DAKISA010000239         2936 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.242, whole genome shotgun sequence.
ACCESSION   DAKISA010000239 DAKISA010000000
VERSION     DAKISA010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.242"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            35..262
                     /locus_tag="OVW45_004443"
     CDS             35..262
                     /locus_tag="OVW45_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCV5528710.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDTHHGPYE"
     gene            complement(263..637)
                     /locus_tag="OVW45_004444"
     CDS             complement(263..637)
                     /locus_tag="OVW45_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secY/secA suppressor protein"
                     /protein_id="HCV5528711.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGDAAADEWDER"
     gene            complement(720..1766)
                     /gene="mdtG"
                     /locus_tag="OVW45_004445"
                     /pseudo
     CDS             complement(720..1766)
                     /gene="mdtG"
                     /locus_tag="OVW45_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            complement(2097..2672)
                     /locus_tag="OVW45_004446"
     CDS             complement(2097..2672)
                     /locus_tag="OVW45_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCV5528712.1"
                     /translation="MSRTFAYCRVSVETSTTENQIQAIKKAGYDIPKSRVIEEVVSGS
                     VSAMDRDGFANLVNHKLEEGDTLVVLKLDRLGRDNIDLQNTVTLLNDKGVKLICLDLP
                     VKDLSSSEGKLVLQLFSTFAEFERNRIRERTKQGLERARVQGKVLGRPIASDTTRAVL
                     EYKERGLSQSQVAKVLNLSVRTVSRHWTKTL"
BASE COUNT          761 a          696 c          687 g          792 t
ORIGIN      
        1 ttatgccggt atttttttac ggaagaggta tcaaatgcgt ttaattgtgg tgagcatcat
       61 ggtgaccttg ctgagtggct gtggcagcat cattagccgc actataccgg ggcaggggca
      121 cggcaaccaa tattatcccg gtgtgcaatg ggatgtgcgt gactccgctt ggcgttatgt
      181 cacaatcctt gatctgccat tctccctggt ttttgatact ttactgctgc cgatcgacac
      241 tcatcatggc ccgtatgagt gattaacgtt catcccactc atcagctgca gcgtctcctt
      301 cctcggtatc cagcggtggc tcaagctgaa attccccctc gtcccattca tgtaatgtat
      361 tctcttcctg ccactcctgg cggatctcta tctcatcata gtcgccatca aaaacacttt
      421 gcgcggcttc accgctaagc ataggtaaac attcaccttc ttccccttcg tcggcaaaaa
      481 actcaacttg ccacatgatg tcgccgtcct gcaaaacgta tttttgggca ttgaactgtt
      541 gcacattcgc atcttcggcg tcgatgccgg ggttgtctgc aagaaattct tcgcgtgcag
      601 cgtcaatggc ttcttcaagc gttgcgtaca tggtcatcac gattctccct ttgagttgat
      661 gaggtttcag ggaaaaggat agctgattct ccgcttttgc aagtatgaaa ggcgaaaaat
      721 cagttcgata cctggggtat tcgacgacga cgtagactgt tccatgaata gactgcgttg
      781 aataacacta cgccagcggt gacgagaaat accgctctga aaccgtagtt cgctgaaatc
      841 gctgctccca tcaatggtcc ggtaacgttg ccaatatcac gaaacgattg gttatagctg
      901 aagatacgtc cggcgatttg gttgctcgag ttataaacta acagtgtctg tacggcgggg
      961 agtagtgcac catcggcggc accgagcaaa aaacgtaaaa tcccaagttg caatggcgtc
     1021 tgaacgtaag acattgggat caacagcagt acagaaaaga tcagcgccgt aatcaggatc
     1081 ttttcgggtc cgattcgatc gccaagtttg ccgagtcgtg gtgcacttag cagagccgcc
     1141 acgcctggca ccgaggcgat catgccactg ataaaggcga cgttactgac gttacccgcc
     1201 agttcgcgga catacagcgt cagaatgggg gcaattgagc ccgtcgccac ctggatgatt
     1261 aacgtagtga caaacaggct gagtaccagt ttcgggtttt taagtgatgt caccacttcc
     1321 cgcatgtgca gcatctcttt tttgctgatc ggctggaatt tttctctgat gcaaaacagg
     1381 gtgacgaaaa agcagagtat gagcacactg gcggtaataa agaataccgg acgtaagccg
     1441 tagctatcgg cgagcaggcc gccagccatt gggccgagca acgcaccact aacgccgcct
     1501 gtggagagcg tacccagcgc ccagccgctt ttattacgcg gtacttgtgt ggcgataaga
     1561 gcattagcgt tggggacaaa tccgccaagt aacccaagaa gcgcccgcag gatcaaaaac
     1621 tgccagatat tttgtgccag ccccatcaac accatcacga tgcccatgcc gagggcagag
     1681 cgtaatagca tgagttttcg gcctttacgg tcggcgagtc caccccaaaa cggtgaggcg
     1741 atggccgaaa ataaaaatgt aatgctcata tatgctatct taatgcacta cctgataagt
     1801 gagcctatac aggttattac tgtatgttta tacagtatta ttggtagaag actgtgtaga
     1861 tttggtatag agatgaatag atactattaa ggtaacttat tgaaatcaat aaagattctt
     1921 gtgcagtctg aatagataac ttagcgttat gtctagtcgt ttccctctcc attcctaaaa
     1981 agtgctgtat actggcctat acaacgctat aaagacctct acatagcgcc ttgatagcta
     2041 tgtcacaggt acaaaaaagc cccctataaa ggaggctgtg tagtctatta agggtcttac
     2101 agggtcttgg tccagtgtct gctaacagtc cttacagaga ggttaagaac cttagctacc
     2161 tgagactgag atagtcccct ttccttgtac tctaagaccg ctctagttgt gtcactcgct
     2221 ataggtcttc caagtacctt accttgaact ctagcacgct ctagaccctg tttagtgcgc
     2281 tctctgatac ggtttctttc aaactctgca aaggtactaa atagctgtag aaccaactta
     2341 ccctctgagc ttgaaaggtc tttaacgggt aaatctaaac agatgagctt cacaccctta
     2401 tcattaagaa gggtcactgt attctgtagg tcgatgttat cccttcccag acggtcaagt
     2461 ttaagaacaa caagggtgtc accttcttct agcttatggt tgaccagatt cgcaaatccg
     2521 tccctatcca tagctgacac tgaaccagag acaacctcct ctataactcg tgacttagga
     2581 atatcatagc cagccttctt aatggcttga atctggttct ctgtggtgga agtctctact
     2641 gatacacggc agtaagcaaa ggttcgtgac atagatatcc ctctgggtag tcattaaacg
     2701 gttagtttgt cttgtctata gacattaaag caaatggtag gtttgttgtc tatagctata
     2761 gtgactcttc tatgtagaca ttaaacggac gtttcttgtc gtgggtcaca tagatacttt
     2821 tagggcttct gtcaagtgat attcgtagat tttgcaaggg ggaggtgtaa ggctgaaaca
     2881 gggctactaa gaacacatac atctaaggct ggaaagtacc tgagcagata ttcttc
//
LOCUS       DAKISA010000240         2879 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.243, whole genome shotgun sequence.
ACCESSION   DAKISA010000240 DAKISA010000000
VERSION     DAKISA010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.243"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..954)
                     /gene="curA"
                     /locus_tag="OVW45_004447"
     CDS             complement(<1..954)
                     /gene="curA"
                     /locus_tag="OVW45_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCV5528713.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGL"
     gene            1135..1653
                     /gene="mddA"
                     /locus_tag="OVW45_004448"
     CDS             1135..1653
                     /gene="mddA"
                     /locus_tag="OVW45_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCV5528714.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            1650..2099
                     /locus_tag="OVW45_004449"
     CDS             1650..2099
                     /locus_tag="OVW45_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCV5528715.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(2100..2333)
                     /locus_tag="OVW45_004450"
     CDS             complement(2100..2333)
                     /locus_tag="OVW45_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCV5528716.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(2419..2592)
                     /gene="ortT"
                     /locus_tag="OVW45_004451"
     CDS             complement(2419..2592)
                     /gene="ortT"
                     /locus_tag="OVW45_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCV5528717.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2787..>2879
                     /gene="yncL"
                     /locus_tag="OVW45_004452"
     CDS             2787..>2879
                     /gene="yncL"
                     /locus_tag="OVW45_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCV5528718.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT          691 a          738 c          692 g          758 t
ORIGIN      
        1 taaaccatca gtaatatctt cgtggtagtg gattttatcc tctttcaccc attgccccat
       61 ctccttctga aactcatgga tgcggtgacc ataatcctga gcgataataa acccttgcaa
      121 gcgaatacgt tttttcagca ctgttgccat caacaggggt aaacgatccg gaccgggtgg
      181 tagctctgta gcgttatagc tgctcactaa gccacaaacg ggaatgcgcg cagatgtatt
      241 aagtaatggt agcactgcat caaatacctt accgcccacg ttttcataat agatatcaat
      301 accttttggg cacgctttca ccagttgttc ggcaaaatca tccgcgtggt gatcaagaca
      361 aacatcgaag cccagcacct cgatagcatg gcggcatttt tccgcgccac cggcaacccc
      421 caccaccctg caacctttaa gtttgccgat ttgccccacc gttgctccca caggacctgt
      481 cgccgcagct accaccaacg tttcgccctc tttaggctga ccgatatcca gcagccccat
      541 ataagcggta aagcctggca tccccagcac acccagcgac cacgatggat tttgcggatg
      601 atcgccaagt ttcaccagat catcaccact ggatatgtca tagtcctgcc atccactgta
      661 gcccagcacc cagtcgccag gctgataatc aggatgattc gactccacaa cacggctcac
      721 cgtgccgccg accatcacgc cgccaatatc aacaggcggt gaataagatg gctcatcgct
      781 catacgtcca cgcatatacg ggtccaggga caaataaact gtgcgcagta acacctgtcc
      841 ttcacccggt gtggcgacat catcttcttc aagacggaaa ttctccggaa ccggtgcgcc
      901 atgtggacgc gaggccagaa cccaacgtcg attacgctgc ttttgttgcc ccataatgct
      961 ctcctgtgct tttggcatga atccatcaat agtagcaggc tcacgggatt ggttttgttt
     1021 gcagccgtca catcccctct gcaacacgca ataatttcat cgaggaatcc gttgcttcgg
     1081 gctgttttcg atagtattga taacatcatc atcattattt tatcgaggcc agacatgtcc
     1141 atccgttttg cccgcaaagc cgattgtgct gccattgcgg aaatttataa ccacgccgtg
     1201 ttgtatacgg cggctatctg gaatgaccaa acggttgatg cagataaccg cattgcctgg
     1261 tttgaagcgc ggaccatagc aggttatccg gtgctggtaa gtgaagaaga tggcgtagtg
     1321 acgggatatg cctcgtttgg cgactggcgt agtttcgatg gttttcgcca taccgtggaa
     1381 cattcggttt atgtccatcc cgatcatcag ggcaaaggtc tggggcgtaa attgttaagc
     1441 cgattgattg atgaagcgcg ggattgcggg aagcatgtca tggtcgccgg gatcgaatcg
     1501 caaaatcagg cctcgctgca tctgcaccat tcgctgggat ttgtcgtcac cgcacaaatg
     1561 ccgcaggtgg gcactaaatt tgggcgctgg ctggatctga catttatgca gttgcaactc
     1621 gacgaacgca ctgaaccgga cgcgattgga tgaatcagtc gctcaccctt gcttttttga
     1681 ttgccgccgg aattggtctg gtagtccaga acacgctgat ggtgcgcatc acccagacct
     1741 cctctaccat tctcatcgcc atgttgctga actcactggt ggggattgtg ctctttgttt
     1801 ctattttatg gtttaaacaa ggcatggcgg ggtttggcga actggtgtcc agcgtgcgct
     1861 ggtggacact tatacccggc ttgctgggat cgttttttgt cttcgccagt atcagcggat
     1921 atcagaatgt gggagcggcg acaactattg cagtactggt cgccagccag ctaattggcg
     1981 gtctgatgct ggatatcttc cgcagtcatg gcgtaccact acgggcgttg ttcggcccaa
     2041 tttgtggcgc aattttactt gtggttggcg cctggttggt agccagacgc tcattttaat
     2101 cagaggatgg tgccgccttt ggttaactgt tcgtggcgcg cttccatatc ttctttatgc
     2161 ttgcgaccgt gatgagcaat agcggtgcgc aaacgctgct gctgggtgta gcgatcttcc
     2221 cgacttaact ccgcgtcatc gctcagggcg attaataatt cgttcatatg ggcaatgacg
     2281 ctctcttcaa tcgcggcatc cacgcgcgcg atgacttcat ccagatgcga catactttct
     2341 ccttaaccac ttgtgaaatt tatcgattca tccaaatgat ataagtctgc gtggtgccgt
     2401 caagccagtg agtaagattt aaaacagtga aaacaacagc gccaccggga aactcatcgg
     2461 ccatgttgcc cccaccagaa aggcgcttaa gaaacggatg cgtttcttat cgtgagaaag
     2521 aaaccaggtg ataagaaatg cgattgctgc cataaccgca taaaaaacaa gcatatgttg
     2581 atagagagac atgtacgggc gtccagccag atgaataaaa cgcgcgcaaa tatgcgtcat
     2641 tttttgacgt gcatcaagtt ttttcaccag tttgagaaaa aatcatcaaa cagcaacttg
     2701 cccttatcca tcttcagaat gagtattatt gcggattttc ttaacccgta ctatacacat
     2761 aggggcaatg ataaaaggtg gcaaaaatga atgtttccag tagaactgta gtactgataa
     2821 atgtctttgc tgctgttggt ttgtttactc ttatctctat gagatttggc tggtttatt
//
LOCUS       DAKISA010000241         2876 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.244, whole genome shotgun sequence.
ACCESSION   DAKISA010000241 DAKISA010000000
VERSION     DAKISA010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.244"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..495
                     /locus_tag="OVW45_004453"
     CDS             <1..495
                     /locus_tag="OVW45_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCV5528719.1"
                     /translation="GADPDYVIWAKEVQGINRAWTFRHWKGTGTVGVMVATNDPERPA
                     PDESVINAVRDHILPLAPVAGSGLYVFGATEKVIPMTIALSKDTPQIRTAIKAELNAL
                     MLRDGVPEGRMYLSRISEAISLSAGEVAHRLIVPSSDIDLGETELPVLGEITWQAYDP
                     ARSK"
     gene            495..1085
                     /locus_tag="OVW45_004454"
     CDS             495..1085
                     /locus_tag="OVW45_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HCV5528720.1"
                     /translation="MDSLQDDYTKLLYGLMPPGPAWSDTDGVLDGLAPSLVRVHQRAD
                     ELVIEIDPGQSTELIERYEELYGLPDSCSPVGTQTLRQRQQRLEAKANVAGGINEQFF
                     LDQLEALGYTGVTIEQFQHLDASPDPEWGDRWRYFWRVTLPMDAGAQWQTCTDACNTP
                     IRTWGDTVAECVINKLCPSHTVVLFSYPDEDEDAQD"
     gene            1072..2112
                     /locus_tag="OVW45_004455"
     CDS             1072..2112
                     /locus_tag="OVW45_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCV5528721.1"
                     /translation="MHRIDTLTAVKDKFGPGKNGFTDGNLRTGRLATWLNSAMWNAIQ
                     EEICGVIEKAGIELNKEEHDQLYKAILLLVGGAINEEALLIKNNLSDVEDRDEAVENL
                     GLKPTVDKAKNAVQRDGDTMTGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSEG
                     QGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGDN
                     DTGFKQNGDGLLDVYANSVHVLRFQSGSIQSNKAVNVTGRVTPSDYGNFDARYQQRNG
                     GVQDVRYGYEMYYTPGSNTVSWTFRSPSGHGLSGISISDTGRNSADNVNGVYYRPLQK
                     LINGTWYNVASI"
     gene            2115..2660
                     /locus_tag="OVW45_004456"
                     /pseudo
     CDS             2115..2660
                     /locus_tag="OVW45_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            2684..>2876
                     /locus_tag="OVW45_004457"
     CDS             2684..>2876
                     /locus_tag="OVW45_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCV5528722.1"
                     /translation="MSVVISGALTDGAGIPMSGCQIILKSRVNTSEVVMRTRADVVTG
                     NNGEYSFEAQVGKYCVYLKR"
BASE COUNT          749 a          627 c          819 g          681 t
ORIGIN      
        1 ggcgcagacc ctgattatgt tatctgggcg aaagaagttc agggtattaa ccgtgcatgg
       61 actttcagac actggaaagg cactggaacg gttggcgtga tggtggcgac aaacgatccg
      121 gaacgcccgg ccccggatga aagcgtgatt aacgcagtca gggaccacat ccttcctctg
      181 gcacctgttg ccggaagcgg attgtatgta ttcggtgcca cagaaaaagt catcccgatg
      241 acgattgcat tatcgaaaga cacaccgcag atcaggactg caataaaagc agagctgaat
      301 gcactgatgt tacgggatgg tgtgccagag gggcgtatgt atctgtccag aatcagtgag
      361 gccatcagtt tgtctgctgg tgaagtggcc caccggttaa tcgtcccttc atcggatatt
      421 gacctggggg agaccgagct tccggtgctc ggcgagatca cctggcaggc ttatgacccg
      481 gcaaggagta aataatggat tcgttacagg atgattatac aaagctgctg tacggactga
      541 tgccgccggg gcctgcatgg tcagataccg acggtgtact tgacggtctg gcaccatcgc
      601 ttgtgcgtgt tcaccagcgg gctgatgaac tggtgattga aatcgatccc ggtcagtcca
      661 cagagctgat tgaacgttat gaagaattgt atggtttacc tgattcctgt tcccctgtcg
      721 gaacccagac attacgccag cgtcagcaac gtcttgaagc aaaagccaat gtggctggtg
      781 gcataaatga gcagttcttc ctggatcagc ttgaggcgct gggatatacc ggcgtaacga
      841 tcgaacagtt ccagcacttg gatgcaagcc ccgatccgga atggggagat cgctggcgtt
      901 atttctggcg tgtgacgttg ccgatggatg ccggtgctca gtggcagaca tgcacggacg
      961 cctgcaacac accgatccgg acgtgggggg atacggttgc ggagtgcgtg attaataaat
     1021 tatgtccgtc acacaccgtc gttttatttt cctatccaga tgaggatgaa gatgcacagg
     1081 attgatacgc tgactgcggt aaaagataag tttggtccgg gtaagaacgg atttactgac
     1141 ggaaatcttc gcacaggacg tcttgctacc tggctgaaca gtgccatgtg gaatgccatt
     1201 caggaggaaa tctgtggtgt cattgagaag gccgggatag aactgaataa agaagaacac
     1261 gaccagctat ataaggctat attattgctg gtgggcggtg caattaacga agaggcattg
     1321 ctgataaaaa ataacctttc ggatgtggaa gacagggatg aggctgttga aaacctcgga
     1381 ttaaaaccca cggtggataa ggcaaaaaat gccgttcagc gtgatggcga caccatgacc
     1441 ggagagctga aaatccgtgg tgttaatgcg ctgaggattt tcaacgaagc ttttggcctg
     1501 atttttcgtc gttcggaaga gtgcctgcac cttattccca ccagtgaagg tcagggggaa
     1561 aatggcgata ttggtcccct gcgcccgttc accattaatc tgcggacggg tgaaatatcc
     1621 atgtcgcata aagtgtctgt tggcggtggt tcgcaggtca atggtgcgct gggtatcggc
     1681 gttcagaacg ccctgggggg gaattcaatt gttcttggtg ataatgacac cggattcaaa
     1741 cagaatggtg atggcctgct ggatgtttat gccaatagcg tgcatgtgtt gcgttttcag
     1801 agtggcagta tccagagtaa taaagctgta aacgttacag gacgggtaac accgtcagac
     1861 tacggaaact ttgatgcccg ttaccagcag cgaaatggtg gcgtgcagga tgtgcggtat
     1921 ggttatgaaa tgtattacac ccccggcagt aacaccgttt cgtggacatt tcgctcacct
     1981 tcgggacacg ggctgtcagg gatatcgata tcggataccg gccgtaactc agcggataac
     2041 gtcaacggcg tgtattaccg accgctgcaa aaactgatta atggcacctg gtataacgta
     2101 gcgagtattt aacaatgttg catttaaaaa atattacggc aggtaatccg aaaaccgcgg
     2161 aataatatca gctgacaaaa cagtatgatg tcacctggct tttttcggaa gacggcaaaa
     2221 actggtatga agaacagaag aactttgcca gtgacaccat aaaaatggtt tacaccggag
     2281 acgggcgcgt ggtgtgggtc ggtaaggatg tgacaggcat tgaaccccgt aacgccagtg
     2341 ttattgaagt tcctgatatt accgccaacc gccgcattac cgcgccgggt tactggtttt
     2401 accgcaatga tgaatttgtc tttgactaca gactcaaagc ggaagatgag cgtgatgccc
     2461 ttctggctca ggtcagtgcc cggacagggg aatgggaaga agacctgctg ctggggctaa
     2521 tcagcgacga agaccgggaa aaactgaaag cgtaccgtat ttacgcgaaa tcgctgcagg
     2581 cgatggattt cagcaccatc actgataaat cctcatataa cgccattgaa tggtccgtct
     2641 ctccggaagg ttcttcctga tttaatttat cgcgagaaaa actatgtctg tagtgatatc
     2701 aggtgcgctg actgatggcg caggcatccc catgtccgga tgccagatta ttctgaaatc
     2761 ccgtgtaaac acctcagaag tggtgatgcg taccagggct gatgtagtga ccggaaataa
     2821 cggtgaatat tcgtttgagg cacaggtcgg aaaatattgc gtgtatctga aacggg
//
LOCUS       DAKISA010000242         2807 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.245, whole genome shotgun sequence.
ACCESSION   DAKISA010000242 DAKISA010000000
VERSION     DAKISA010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.245"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            21..1103
                     /locus_tag="OVW45_004458"
     CDS             21..1103
                     /locus_tag="OVW45_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005099906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCV5528723.1"
                     /translation="MFRIPVNQWTLATAALGGVFLVSGLILLMNYNHPYTFTAQKAVI
                     AIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKTL
                     RAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDALV
                     KGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLIR
                     PGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGKL
                     TSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYSD
                     HFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1563..2000)
                     /locus_tag="OVW45_004459"
     CDS             complement(1563..2000)
                     /locus_tag="OVW45_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528724.1"
                     /translation="MADKAILWALISASTKEGRKACSLSYFACKAAEAELGLAYMAAN
                     DNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGKIIRPYLKNLNPLQLAADCIETVNK
                     IKDKNKKIIDINSVNICSDDKNIKLRVNSTIMAIDDSIKCIDE"
     gene            complement(2089..>2807)
                     /locus_tag="OVW45_004460"
     CDS             complement(2089..>2807)
                     /locus_tag="OVW45_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2778 domain-containing protein"
                     /protein_id="HCV5528725.1"
                     /translation="LVLISTEGATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDE
                     ESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNPITDIDPLGLATCLYSITL
                     SMLSCVSDTQNDDNSYDVLTIPVASGNNGNNMQCKNNPGCTHLQNRGPIPQGVWSWNV
                     NGPGATNRKPNGIRLVPSANTETYNRDGFLTHSCLNAFGPSLGPRFCSEGCITGSSND
                     MQKLNELIFSEPDSTLTVTD"
BASE COUNT          748 a          603 c          617 g          839 t
ORIGIN      
        1 tggcgtgggc ggtctttaaa atctttcgta ttccggtgaa tcagtggacg ctggcgacgg
       61 cggcgctggg aggcgtgttt ctggtgagtg gtttgatttt gttgatgaac tacaaccacc
      121 cttacacttt taccgcgcaa aaggcagtga tagcgatccc tatcacgcca caggtgacgg
      181 gaattgttac tgaagtcact gacaagaata atcagcttat tcaaaagggc gaggtgcttt
      241 ttaagctcga cccggttcgt taccaggcgc gagttgacag acttcaggct gacctgatga
      301 cggcgacgca taatataaag acgctgcgtg cgcagctcac tgaagcgcag gccaacacca
      361 cccaggtttc agcggagcgc gaccgtctgt ttaaaaatta tcaacgttac ctgaaaggca
      421 gccaggcggc ggtgaatccg ttctcggaac gtgacatcga cgatgcgcgg caaaatttcc
      481 tcgcgcagga tgcgctggtg aaaggctcgg tggcggagca ggcgcagatc cagagccagc
      541 tcgacagtat ggttaacggc gagcaatcgc agattgtgag cttaagagcg caacttactg
      601 aagcaaaata taaccttgag cagactgtca ttcgcgcgcc gagcaatggc tacgttactc
      661 aggtactgat ccgcccaggt acatacgcag ctgccttgcc gctgcgtccg gtgatggtct
      721 tcatccccga gcaaaaacgg caaattgtcg cccaatttcg gcaaaactcg ctgttacgtc
      781 tgaaacctgg cgatgatgcg gaagtggtgt ttaacgcgct acctgggcag gtgttccacg
      841 gcaaattgac tagtatttta cctgtcgtgc caggcggttc ttatcaggcg cagggggtat
      901 tgcaatcatt aacggtcgtt cccggcacgg acggcgtgct gggaaccatt gaactggacc
      961 ctaacgatga tatcgatgcc ttacccgacg gcatttacgc ccaggtagca gtttactccg
     1021 accatttcag ccatgtttcg gtgatgcgga aggtgctgct aagaatgacc agttggatgc
     1081 attatttgta tttggatcat tgagggggaa gggggagatt atggaacttt gctccctgca
     1141 agggctaatt atttttgcat attacttcat tggctgcgat gatttttctt taatattcat
     1201 tatgttaata agttctggat gagtgtgctc tcattggatt ttttatccgt cttgttctgc
     1261 cgcaggatga gactatacat cgtcgagatc gcaatgatga tatgagttgt tcctcctgcg
     1321 acggctctaa tcaaaaaaga gcaggagtgc ttttccatct attgcgatga tggctttatt
     1381 atctgaagga tgacagtcac atttaaaaaa tatttaatct atagatcaca atgtagggtc
     1441 atggaatata tgtatacaac tattttcttc ttcactttta attatcgtat tcattataaa
     1501 actaagttaa aaaaactcct agagagtgag ttattaatat atttttaaat aaaattaagt
     1561 tatcattcat cgatgcattt tatagaatca tcaatggcca taatagtact attaactctc
     1621 aattttatat ttttatcatc actacaaata ttaactgaat ttatatcaat gattttttta
     1681 ttcttatctt ttattttatt tactgtttca atgcaatcag cggcaagttg gagtggattt
     1741 agatttttca gatatggtct tatgatcttt cccttactta ataaatagca tgtataggat
     1801 tcggaaagtc cagcatctat tttgtacctc attatattag ataaggatgt gagaaattcc
     1861 ttgttatcat tcgcagccat atatgccagt ccgagttcag cctctgcggc cttacaggca
     1921 aaatagctta gtgaacacgc ttttcgtccc tctttagtac tggctgatat tagtgcccat
     1981 aatattgctt tgtctgccaa atctaactct ctctgaaatt gtcttgcagt aactgcactc
     2041 gcagaaatag agaaaaaaat acaattgtta ataactttgc catatttttt agtccgtaac
     2101 tgttaatgtg ctatctggct ctgaaaatat taactcattt aatttttgca tgtcattaga
     2161 tgaaccagtt atacaaccct cggaacagaa tcgaggtcct aaagacggac cgaaagcgtt
     2221 taaacaacta tgtgttaaaa atccatcacg attatatgtt tctgtatttg ctgacggtac
     2281 taaacgtata ccattaggtt ttctattggt tgctccagga ccattaacat tccagctcca
     2341 taccccttga ggtattggac ctctattttg taagtgtgta caaccaggat tatttttaca
     2401 ttgcatatta ttaccattat taccagaggc gacaggaata gtaagcacat cataagagtt
     2461 atcatcattt tgcgtatctg aaacgcatga aagcatactt aaagttatgc tgtataaaca
     2521 ggtcgctaat cccaacgggt caatatcagt tatcggattc aacggatact gataaaaatt
     2581 ccatcccccc ttcagtccaa tcggatcctg agtgatatat cgcccctgca gcgggtcata
     2641 atagcggtgg cggttgtaat acaggccgga ctcctcatca tactgctgcc ccggcaggcg
     2701 gataagctgc tgcagctgat gcgggttctc ttcattcagc aggttgcccc actcatcgta
     2761 ttctgcgcac cattctgttg ccccttccgt gctgataagc accagcg
//
LOCUS       DAKISA010000243         2801 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.246, whole genome shotgun sequence.
ACCESSION   DAKISA010000243 DAKISA010000000
VERSION     DAKISA010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.246"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..621
                     /locus_tag="OVW45_004461"
     CDS             <1..621
                     /locus_tag="OVW45_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HCV5528726.1"
                     /translation="SGTKISQRECTDILFAEMKELAKMFSFFGQYKTLIEDLIEHFRY
                     GNGSNFHSQQLNLSFHEKINKYGYNSPIRIIKECIENGINSTPSTGYQPLILQSIKTK
                     LLSSRLNKFNDFEDSFNGLGISVHDISAQKISLLSFQKYAIGWSATIHFVAQDHFGLD
                     VTDIKNKTYSKYRFFRIWFFLQRHKDFAFKPFFTNFNTIERIENYL"
     gene            618..1097
                     /locus_tag="OVW45_004462"
     CDS             618..1097
                     /locus_tag="OVW45_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HCV5528727.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFK"
     gene            complement(1151..2101)
                     /gene="corA"
                     /locus_tag="OVW45_004463"
     CDS             complement(1151..2101)
                     /gene="corA"
                     /locus_tag="OVW45_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCV5528728.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            2471..>2801
                     /locus_tag="OVW45_004464"
     CDS             2471..>2801
                     /locus_tag="OVW45_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528729.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPMLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLVDMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
BASE COUNT          845 a          620 c          591 g          745 t
ORIGIN      
        1 tccggaacaa aaatatcaca acgtgaatgc actgatattt tatttgcaga aatgaaagaa
       61 ctagcaaaaa tgttttcctt ttttggacaa tataaaacac ttatcgaaga tctgattgaa
      121 cattttagat atggaaatgg cagcaatttt catagccaac aattaaattt atcttttcat
      181 gaaaaaataa ataaatatgg ttataatagt ccaatacgaa taatcaaaga atgtatcgaa
      241 aatggcatta attcaactcc ctcgacagga taccaacctc ttatcctgca aagcattaaa
      301 accaaattac tttcatcaag actaaataaa tttaatgact tcgaggacag ttttaatggc
      361 ttaggaatca gtgttcatga tatttcagct caaaaaatat ctcttttaag ttttcaaaaa
      421 tacgctatag gatggagtgc aacaattcat tttgtagcac aggaccactt cggtcttgat
      481 gtaactgata tcaaaaacaa gacttacagc aaatatagat tttttcgcat atggttcttt
      541 ttacaacggc ataaagactt tgctttcaaa ccttttttca ccaattttaa cactatcgaa
      601 agaatcgaaa attatctatg aaaagcaaac tccagaaaat tatattatgt ttatttcttc
      661 tatgctgcat ctataattta tggacattgc gtcctgtaca gattttgtat acatactctg
      721 atgctggcaa ttcagtcttt ctggtcgttg atcatctgcc atggactgac agcgataaaa
      781 taaattggta tctgaagcat cagaatgaaa ttaaaaatca acatccccta ccagaaggta
      841 gttggcatac ctggtatgtc attgatatcg gtaatggctt cactgactat aaaaaatata
      901 tcgaagggcc gtatgaagat ctctactgtt tcccgacaat taagagcaat gataattgca
      961 tcgtgaaaaa ttacttaatg gttattaatg aataccctta tcgtaacact catattggta
     1021 ttaacgattt tactgagtat caattaacac aagaaaataa aatagagcga gttttcaatc
     1081 cgcatgactt taaataagac aatttttaga aataagccgg ataaaaaaat agctttatcc
     1141 ggcactcata ttacagccag ttcttgcgct taaagtacag atacggtgcc aggcccgcga
     1201 ggatcataaa gataatcgcg cctgggtagc cgaaactcca cttcagttct ggcataaact
     1261 caaagttcat cccatagctg gaagcaacga gcgtcggcgg caggaatacc acggatacca
     1321 ccgagaagat tttgatgatg cggttctgct cgatgttgat aaaacccatc gccgcctgca
     1381 tcaggaagtt caccttctgg aacagggatt cgttatgcgg cagcagggat tcgatatctc
     1441 gcaggatttc acgcgcctgc tccagttgcc cacccggtaa acgcgcttta cgcaccagga
     1501 agttgagcgc gcgctgggta tccatcagac acaggcgaac tttccagccg atatcttcca
     1561 gttccgccag agtggagagc gcctcgtcgt actcatcgcc ctgatgccct tccataatca
     1621 cccggctcaa ctgctccagg tcgctataga tattttcaat ttcatctgcc aactgttcga
     1681 ttttggtttc gaacagatcc agcagcagct cgtaggcgtt accgtctacc atcgactggc
     1741 tacgggcacg catacgatac agacgaaaag cgggcagttc acgctcacgc agagtaaaca
     1801 gacgaccatc acggatggta aatgccacag tggagttacc ggcgtgatct tccgcatctt
     1861 caaagaagaa gaaggagtga atatgcaggc cgtcgtcgtc ttcaaagaaa cgtgccgatg
     1921 cttcgatgtc ttccagttcc gggcgggttg ccaggctctg gccaagttca gattgtacgc
     1981 gcagtcgctc gtcgtcgtcc ggttcgacaa gatcaatcca tactgcattt acaaggggtt
     2041 gtgactcttc gacttccagc cgggtcagtc ggttattttc cagttgaaat gcgctcagca
     2101 tgaccgggac tcccaatgct taaaatatcg gacagttcgg tgggcacaca gaaacaaatt
     2161 gggtttcaga ccattaaaca gcctgactca gcgcgacggg aaaaattgag gtcgctgaca
     2221 accgctaagg ctatcagcaa aaagggatag ccttaggagt tgatcctgga tgacaggata
     2281 gtgagccagt atctactggg tgtgtccaag gcgaatgtcc tcttagagtg atcgtgcgcg
     2341 catgttacgc cagcaaaatt ttgccgtcaa cacgcaacag aacaccacag aacaatagtt
     2401 gaacttcgcg ctttttggta ctcgtcaagg tcgctaacag gataaaaaaa tccaacatcc
     2461 aggacctata atggcgcatc ggctactcat tggtaaagga atgatcacct tgaatctcaa
     2521 acggattttt ctcgccctca ccttacttcc cttgtttgct gttgccgctg atgattgcgc
     2581 actctcagat ccgatgctga ccgtacaggc gtataccgtt aatcctcaaa cagagcgggt
     2641 gaaaatgtac tggcaaaaag ccaatggcga agcgtgggga actttacatg ctctgctggt
     2701 ggatatgaat agtcagggtc aggtgcagat ggcgatgaac ggcggcatct atgatgaaag
     2761 ctatgcgccg ctcggtttgt acatcgaaaa cggtcagcag a
//
LOCUS       DAKISA010000244         2743 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.247, whole genome shotgun sequence.
ACCESSION   DAKISA010000244 DAKISA010000000
VERSION     DAKISA010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.247"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..120
                     /locus_tag="OVW45_004465"
     CDS             <1..120
                     /locus_tag="OVW45_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCV5528730.1"
                     /translation="RPVKASSEAVQTLISQGKTRLQIQEELGISRATYYRLAK"
     gene            2068..2625
                     /locus_tag="OVW45_004466"
     CDS             2068..2625
                     /locus_tag="OVW45_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528731.1"
                     /translation="MQYSEFRAMLQASRERNKHNSYAYTNNPTSYEVPTFSKSERKNI
                     EAVIRSITPRDRFMPVRKTTKNTIKNYLANFDSYEQLPSKLDDVFIGFCRSEGHPKYN
                     KKLFYLLKNLDEINASTVTNHLQRQATRLSYELPSDKYCALLAVMCAKLIGIVEHHIA
                     VGNIEPMENEQPDFEFDPYLVTEDF"
BASE COUNT          898 a          530 c          556 g          759 t
ORIGIN      
        1 aggcctgtaa aagcttcttc agaggctgta cagacgctta tttcacaagg taagacgagg
       61 ttgcagattc aggaagagtt aggaatctct agagcaactt attacagact tgctaaataa
      121 gttactgaat agttacatgg atgtagctga tatttggctt ttttgccaag ggggtcacat
      181 agctggtgtg acgggtactt agcagctaca gacatctgtc agatattttt gaagattcgg
      241 ggaagatgag attcaaagag ggctagtcat tttttataaa aattttcaga ttctgaaaag
      301 ttactgtaca gattttgcac aatcctacca aacctcatag ttgattcgat tagaagctaa
      361 tctcatagaa agctctgtag cgagttttaa ccccattttt aggggtaacc tatagggatg
      421 tactaaccac cttaaaataa atgtactaac caccttaaaa taaaggggta aaatcgctta
      481 gaatgcgtta cagagcgtct gagaagccat attcacagat tttactaaaa cctcagactt
      541 ctaagtagtc ttttaacagg aggggttccc ctatggtcac atatcccctc tctttccact
      601 aagtaagcct tagcagcacc taagcaacaa ttgactaaca gattcggtag agtctatctc
      661 ataggtcaac ttcataaact cagagctaag ttaccttgaa agattctacc aagaactaca
      721 caggtgttat ttagtctgac tgtgaaggga tatttaacgg gagaagagat acgatataac
      781 ccgaagggag agtatcatct taacaattat cttaagaata aaaactgaaa aactcttaac
      841 agataaccac ctagaaagaa gggaacgata agaccagagg tagtgacctg ttaaacaggc
      901 tgtttcaaag tctcttaaaa gtctgttaag taaggtcatg tgaacaatct taccattttg
      961 tcaatgcttt tgtctttgac ctaaatcaat aaaatgtatc gtttgatatg cttttaaaca
     1021 aaatagctaa aaaatctttt ttagaggggc gcttcgcttg gggtcactat gaatgatttt
     1081 tctctcgcta agagagcgct tacagaggtc tttcaaatct caatagccag ccgaggaaca
     1141 caggaaaccg agggaagaaa aactattgtg attaccttga acaaggtctg taaaggtcgt
     1201 ttaagaggtc ggttaagaaa ggagcttccc tctctggggt tatcgctctt ctcccgatta
     1261 gtggggcggt taagggggct tgaagttatc tgtgaaggta ctggacagtc ttatatattg
     1321 ggtgattaca actcattgaa aaataagcat ttttagaagt tactgttaag gttgcagagt
     1381 tgtctgtgaa gagacttgtc agttctctat atatggctaa ttacaactta ttgaaaaata
     1441 agaactttta gaaattactg tcagagaggt gaatgcttga tagacacagc tttccggttc
     1501 tgtcaagcta ttttttaata gatagcacaa aatgctaaag acacccctgt accatagact
     1561 tctatctgtc atctcgtgca ggggttttga aggagtttaa cgggggtctg tgtggtcgtt
     1621 atccttaagg tcaagcgtcc atgtacgacc gaacagttta aagacaacaa gcactctgac
     1681 gaggacgtag cgaagtagca ggcctgcaat cagcccaata cctccaccag tccatacatc
     1741 aggtgacccc agctcaggag ggagatttac cattagtttt ccttgtaaga cgctctcaat
     1801 cgcattctga cacaaaaagt acctgacctt atgagttgac ctgaagaggt cgtttaactc
     1861 gttacagggc gattgaagag gtctgtgtgc aatgcaaaat atgcgaccaa atacatagcc
     1921 acgcctcata ttttactctc cccataaacc cattatacca cagatgttac gaaaatgtta
     1981 aaaatacttg acattagtct ttgaaaatgc taatattgta tatataggtt aacagaataa
     2041 taacatcttc tgagagtcag aacatgaatg cagtattcag aatttagagc gatgcttcag
     2101 gcatcaagag aacgcaacaa gcacaactcc tacgcttaca caaacaaccc caccagctac
     2161 gaagtcccaa cctttagcaa atccgaacgt aagaacattg aagcggtcat tcgtagcatt
     2221 acgccacgag acagatttat gccagtacgc aaaacaacaa agaatactat taaaaattat
     2281 ctggctaact ttgacagtta cgagcagtta ccaagcaagc ttgatgatgt ttttattggg
     2341 ttctgtagaa gtgaaggcca cccgaaatac aacaaaaaac tattttatct gctaaagaat
     2401 cttgacgaaa ttaacgcatc caccgtgaca aatcatcttc agcgacaggc aaccagactt
     2461 agttacgaat taccaagcga taaatattgc gctttgcttg ctgtgatgtg cgcgaaatta
     2521 attggcattg ttgaacatca tattgccgtt gggaatattg agccaatgga aaacgagcaa
     2581 ccagatttcg agtttgaccc ctatttagtt acagaggatt tttaaatgaa taaacacaca
     2641 ttaaccagag ttacaaagac agcctttgat gaactgaacc gaatcaaaaa cattacaggt
     2701 ctgccacact cagcagtgat gaagcttgca attaaccgtg ata
//
LOCUS       DAKISA010000245         2730 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.248, whole genome shotgun sequence.
ACCESSION   DAKISA010000245 DAKISA010000000
VERSION     DAKISA010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.248"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(423..680)
                     /locus_tag="OVW45_004467"
     CDS             complement(423..680)
                     /locus_tag="OVW45_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528732.1"
                     /translation="MSDHVIECASRAGRDFSEFMKGEKGMMEALASVDEFGEQLRLNG
                     CVNHHFVSYMMRNSIMQAFMDMAKAERKEERRRKRAEAKAK"
     gene            complement(677..1174)
                     /locus_tag="OVW45_004468"
     CDS             complement(677..1174)
                     /locus_tag="OVW45_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HCV5528733.1"
                     /translation="MKSLTLFNQPIRIGEDGMICLTDMWKASGKSESESPYHYLRNKQ
                     TKEFLAELEKNHESVVFTERGVHGGTYGGKFVAYDYAAWLNPGFKYAAYKVLDDYFTG
                     ELQHRNSLSAQLNMKCHEFDQKKDMASFCGQGLAAWRYTKPVLVAEINSLANQLQITI
                     PGLPG"
     gene            complement(1376..1834)
                     /locus_tag="OVW45_004469"
     CDS             complement(1376..1834)
                     /locus_tag="OVW45_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCV5528734.1"
                     /translation="MSRVTAIISALVICIIVCLSLAVNHYRDNAIAYKEQRDNKASEL
                     EKANATIADMRKRQRDVAELDARYIKELADANATIESLRADVSAGRKRLQVAATCAKS
                     TTGASGMGDGESPGLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLK"
     gene            complement(1831..2328)
                     /locus_tag="OVW45_004470"
     CDS             complement(1831..2328)
                     /locus_tag="OVW45_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCV5528735.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPGGNDGLEGVRHNPYKE
                     IVGVWTVCYGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGNQWKGLMTRREIEREVC
                     LWGKQ"
     gene            complement(2328..2543)
                     /gene="essD"
                     /locus_tag="OVW45_004471"
     CDS             complement(2328..2543)
                     /gene="essD"
                     /locus_tag="OVW45_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HCV5528736.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGG"
BASE COUNT          648 a          709 c          620 g          753 t
ORIGIN      
        1 atacagcgca tgatgtgtca ggcttgaata cctttatcct ttaaaaggga tatcagttaa
       61 gttatcccgt gtagggtata agccattatc aaagccactc tgtagggaat ggcttttgtg
      121 atggcatcac ttactcttta cgctgctatc ccactcatcc cggaattttg atgggttatt
      181 gaaaccttct gctgacataa caactccttc aatgtttggc tgaaattggg atgtctttcc
      241 atcagtccgc caccacaaag aatctttttt gccataaggc aggaggttca tctttcagtg
      301 gctgccggtg ttatttcccc acttactggc ttgggttgtt tcgtggtact gccgttaact
      361 ggtggcccag aataaattcc ggtttcatta tcaagcccac ccgtaaatgg gctttgtaat
      421 ggctacttcg cttttgcttc cgctcgctta cgccggcgct cttctttcct ctcggctttt
      481 gccatgtcca tgaatgcctg catgatcgag ttccgcatca tgtagctaac aaagtgatga
      541 ttgacacagc cgttgaggcg cagctgctcg ccaaactcat ccaccgaggc caatgcttcc
      601 atcatgccct tctcgccttt catgaactct gagaagtcgc gccccgctct ggaggcgcat
      661 tcaataacat gatcactcat cccggaagcc cggggatcgt aatctgcagc tggttagcca
      721 gggagttaat ctcagcgacc aacactggct tcgtatagcg ccatgctgcc agcccttgtc
      781 cgcagaagct cgccatgtct ttcttctggt caaattcatg acatttcatg ttgagctgcg
      841 cacttaagct gttgcgatgc tgaagttctc cggtgaagta gtcatcgagg actttatagg
      901 ccgcgtactt gaacccgggg tttaaccaag ccgcataatc gtaagcaaca aacttcccgc
      961 catatgttcc accgtgtaca ccgcgctcag taaaaaccac agattcgtgg tttttctcca
     1021 gctcggctaa gaactctttg gtctgcttgt ttcgcaggta gtggtaaggc gattcagatt
     1081 cacttttacc actggctttc cacatatcag tgaggcagat catgccatct tcaccgatac
     1141 gaattggttg attgaagagg gttaatgatt tcatagcgtg tacctactct ttgaaatgaa
     1201 cctttgccgc acaggaaacc agcccaccga ggctcgccag cactaactgg tatcctcaaa
     1261 ggcccattcc aaaggggcag gttcggtgta aaaaacatgc gttgcggtac gcatttattg
     1321 caaaaagccc cgcatcgcga ggctcattaa atggactttg tgatttgcaa aaaaattatt
     1381 tcaggcattg cgtcctgatg tattcctgca ggtagttaac ctgcgcggtt atcttgtcga
     1441 ttccacttcg gagacggtaa taattgagtt cagcatctgc tgtaagtcct gggctttctc
     1501 catcgcccat gccgctggct ccggtcgttg actttgcaca ggtggcggcg acttgcaggc
     1561 gcttacgccc agcagaaaca tcagcacgga gactttcgat agtcgcgtta gcatcagcaa
     1621 gctcctttat gtatcttgcg tcgagttctg ctacatcacg ttgacgcttc cgcatgtcag
     1681 cgatggtggc gttcgccttc tccagttcac tggccttgtt atcgcgctgc tctttgtagg
     1741 cgattgcgtt atcacggtaa tgattaacag ccaatgacag gcagacgatg atgcagataa
     1801 ccagagcgga gataatcgcg gttactctgc tcattgtttc ccccacaaac agacttcacg
     1861 ctcaatctca cggcgagtca tcagtccttt ccattgatta ccgccagcgt atgtccagcg
     1921 ccgtagctga tcacatgcgc ctttaatatc accctggttt attttgcgaa gaagcgtcga
     1981 tgttctgaaa ttgccagcac ccacgttgta gacgaacgag taaagagcgc cgcgcgttgt
     2041 ttccggtata tcgactttga tgtacgggtt aatttgtctg gcgacagtgg caaggtcttt
     2101 attcaggagg gctttgcact ctgcttcggt atacgtttta ccgggaatga tgtcttttcc
     2161 tgtatgcccg taacatacag tccatacacc aactatttct ttgtaaggat tatgtctcac
     2221 accttccaga ccatcgttac cacctgggcc agtgattaac acagatgcta tagcaatagc
     2281 cccgccacca atagcagcag caacagcttt tcgtaatgat ggaggcatta tccacctctc
     2341 gcagccttgc gcttatcttc tttaatcttg aaataaaggt ttgtcaggta cgtcagcagg
     2401 ccaaatacca ggctacccag cactccaatt gccgcccact gtgagggcgt gactttatct
     2461 agcagctgta aaaaccagta cccggcacta cctgctgagg tgccataggc gacacccgtt
     2521 gttaacttat ccatggattt cataacccca cctcgcagac aaagcgggtg taaattgagg
     2581 gaatactacg aaacgtaaca gactcggagt cagtgaataa ctcaggtatt gggttatcag
     2641 ctaatatcga gactcaaaaa atggaaaaac ccgctcgacg gcgggtttaa gctgtgtgac
     2701 gaagtaacca ctcttaacag cataaccaat
//
LOCUS       DAKISA010000246         2690 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.249, whole genome shotgun sequence.
ACCESSION   DAKISA010000246 DAKISA010000000
VERSION     DAKISA010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.249"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            230..769
                     /locus_tag="OVW45_004472"
     CDS             230..769
                     /locus_tag="OVW45_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCV5528737.1"
                     /translation="MSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDNDFTK
                     GLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIYRKEY
                     ATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKVINES
                     GVKYDVIHFGACRVNREEK"
     gene            complement(995..1318)
                     /locus_tag="OVW45_004473"
     CDS             complement(995..1318)
                     /locus_tag="OVW45_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HCV5528738.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1604..1798
                     /locus_tag="OVW45_004474"
     CDS             1604..1798
                     /locus_tag="OVW45_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCV5528739.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEI
                     AEELQNSIQTRFEAESGTGV"
BASE COUNT          857 a          665 c          579 g          589 t
ORIGIN      
        1 aaaaaaaaca ataaaaaaca ccgcaaaaat ggattgttaa caaatgaaaa tactcaattc
       61 gaatctcata acaaacatat catcaacaat aagaaatcac cttctaacat catccaaaaa
      121 tataaaaaca cacccatcac caaacatgga cgtaacaagt catcgtcgtt tattccagaa
      181 agacggaata ggattgagcc aaacaggaaa agcaacacaa cttgtcatta tgtcgcatgg
      241 tggatggaaa gagatctccc atccacaaac actattcacg cgtcaaaaag gcgatggatg
      301 gactgtagtc cccaaaaacc taagaattga tttttataca aaagacaacg acttcacaaa
      361 gggactaagc gttctttcag aggtaaacaa acgccatact gaagctcaaa aagggttaac
      421 accatcacta aacataagca aagatgacct aaaagttcta gcgaacgcgc gcaatatacc
      481 tcaatcaaaa ttagaagaag aaatgatgtc acaagccata tatagaaaag aatacgcaac
      541 aagtaatgag aagataaaaa actatgctct atattaccat gaacaagccc aaaatattat
      601 acaaagacat caagatggct taggaaacaa aaatattgat atagcattta taactgataa
      661 aaatcataag aagcaccttt ctgacatatt taaagtaata aatgaatcag gagtaaaata
      721 tgatgtaatt cactttggcg cttgccgagt aaatagagag gagaaataat catcatttac
      781 cataatccct taattgtacg caccactgaa acgcgctgaa cgcgattctg acgcagacac
      841 cacaaaaata gatgttttac cccggaaaaa caggaaggat gcgtcaggat cgttttcagc
      901 tcgctgcata gctatgcatg aaagcgaatg gagatcactt tgggagctta ccgtgttcat
      961 accgtctgtt ttcaacagtt ttctccccgg gaagctaatc tgccataagc ctggataaca
     1021 gggcacggtg atactccgta atagcgatca gcacctccgc atactccctg tccccgatac
     1081 gcttgccgtc gataaagagt tttttctgta acgcccccag tctcataact tctttcagca
     1141 cccgatcatc agtcagtgag ttaacgttct taccgagagc tgccgcccgt aaaaaaccag
     1201 aaacggtctt gccgcattca gcagcccttt ttctgatttc ctggtcttct tctgccgtca
     1261 gacgaacagg gcgactgata gcccgcctgc gcgtattact tccacttcgt tttgtcataa
     1321 aacatggctc cgtatctgac acggggtgtc ggggcgaagc cctgaccagg gtaattgtaa
     1381 tagcgtgcat gtatgcgcgg tataacaatt acacatcctg tcctgtcagc aagttcgaat
     1441 taccgacaaa ggcacggcag tttttttggc agtgacagta ttgtcaggca gaaatacccg
     1501 tcaatgcgtc agacggcaga ataccggcgg taaaacagtc gaaaagtggt acgcgatata
     1561 atcacacaac gcagatgaca gacagaacgg agaaaacaga aagatgaaca agcagcagca
     1621 aaccgtactc aacatggcgg gattcataaa aagccagagc ctgacgctgc tcgaaaagct
     1681 ggacgcactc gatgccgacg aacaggccgc catgtgtgag aagctgcacg aaatcgcgga
     1741 agaactccag aacagcatac agacacgctt tgaagcggaa agtggcaccg gggtataaca
     1801 gtgtatcctg ctccagacag gatgcagaac ggtataccgg tgcgtaagga gaaaatgtca
     1861 cataaggcgc tcttccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
     1921 gcgagcggtg tcagctcact caaaggcggt aatgcggtta tccacagaat caggggataa
     1981 cgccggaaag aacatgtgag caaaaagccg aaggcaggga caaaggccgc gcaggtggcg
     2041 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2101 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2161 cgctctcctg ttccgaccct gccgtttacc ggatacctgt ccgcctttct cccttcggga
     2221 agcgtggcgc tttctcatag ctcacgctgt tggtatctcg gcccggtgta ggtcgttcgc
     2281 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2341 aactatcgtc ttgagtccaa cccggtaaga cacgaattat cgccactggc agcagccact
     2401 gataaccgaa ttagaggagt aagacttgaa gtcatgcgcc tgttaaggct aaactgaaag
     2461 aacaagtttt ggtgactgcg ctcctccaag ccagttacct cggctcaaag agttggtagc
     2521 tctgagaacc ttcgaaaaac cgccttgcag ggcggttttt tcgtttacag gcagcagatt
     2581 acgcgcagaa aaaaaggatc tcaagaagaa cctttaattt tttttcacac ttggtatcct
     2641 actttaaccc ataaaataaa cacttacacc gacacaccct taaacaaaag
//
LOCUS       DAKISA010000247         2605 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.250, whole genome shotgun sequence.
ACCESSION   DAKISA010000247 DAKISA010000000
VERSION     DAKISA010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.250"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            1..2605
                     /gene="rhsB"
                     /locus_tag="OVW45_004475"
                     /pseudo
     CDS             1..2605
                     /gene="rhsB"
                     /locus_tag="OVW45_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="rhs element protein RhsB"
BASE COUNT          606 a          797 c          804 g          398 t
ORIGIN      
        1 tactgaccac gcaggcgcag cgggcagaag aagcccggca gcaggccatt tccggcggga
       61 cggaaccgtc cgcttttcct gataccctgc cgggttacac cgaatatggc cgggacaacg
      121 gcatctgtct gccgtgtggc tgacgcacga cccggaatac ccggagaatt tacctgccgc
      181 gccgctggtg cgctatggct ggacgccacg cggcgaactg gcggtggtgt atgaccgtag
      241 tggcaaacag gtgcgcagct ttacttacga tgataaatac cggggccgga tggtggcgca
      301 ccgtcacacg ggccggccgg aaatccgtta ccgttacgac agcgacgggc gggtgacaga
      361 acagcttaac ccggcaggct taagctacac gtatcagtat gagaaggacc gcatcaccat
      421 caccgacagc ctgaaccgcc gtgaagtgct gcacacgtag ggtgaagccg ggctgaagcg
      481 ggtggtgaaa aaggaacacg cggacggcag cgtcacgcag agtcagtttg acgccgtggg
      541 caggctcagg acacagacgg atgccgcagg ccggacaaca gaatacagcc cggatgtggt
      601 gacgggcctc atcacgcgca tcaccacgcc ggatggcagg gcatcggcgt tttactataa
      661 ccaccacagc cagttaacgt cagccaccgg gcctgacggg ctggaaatgc gccgcaaata
      721 tgatgaatcc ggccgtctga ttcaggaaac cgcccctgac ggcgatatca cccgctaccg
      781 ttatgataat ccacacagtg acttaccctg cgcaacggaa gatgccaccg gcagccggaa
      841 aaccatgaca tggagccgtt acggtcagtt gctgaccgtc actgactgtt ccggttatgt
      901 aacccgttat gaccatgacc gttttggtca ggtgacggcg gtgcaccgcg aggaagggct
      961 gagccagtac cgcgcatacg acagccgtgg acagttaatt gcggtgaaag acacgcaggg
     1021 ccatgaaatg cggtatgaat acaacgccgc cggtgacctg accgccgtca ttgccccgga
     1081 cggcagcaga aacgggacac agtacgatgc gtggggaaaa gccatctgta ccacgcaggg
     1141 cgggctgacg cgcagtatgg aatacgatgc tgccggacgg gtcatccgtc tgaccagtga
     1201 aaacggcagc cacaccacct tccgttacga tgtactcgac cggctgatac aggaaaccgg
     1261 ctttgacggc cgcacacagc gttatcacca cgacctgacc ggcaaactta tccgcagcga
     1321 ggatgagggg ctggtcaccc actggcacta tgacgaagca gaccgcctca cgcaccgcac
     1381 cgtgaagggt gaaaccgcag agcggtggcg gtacgacgaa cgcggctggc tgacagacat
     1441 cagccatctc agcgaagggc accgggtgac ggtgcattac gggtatgatg agaaaggccg
     1501 gctgaccggt gagcgtcaga cggtgcatca cccgcagacg gaagcactgc tctggcagca
     1561 tgagaccaga catgcgtaca acgcgcaggg gctggcgaac cgctgtatac cggacagcct
     1621 gtccgccgtg gaatggctga cctacggcag cggctggctg tcaggcatga aactcggcga
     1681 cacaccgctg gtggagtaca cccgcgaccg cctgcaccgg gaaacgctgc gcagcttcgg
     1741 ccgttatgaa ctcaccaccg cttatacccc tgccgggcag ttacagagcc agcacctgaa
     1801 cagcctgctg tctgaccgcg attacacctg gaacgacaac ggcgaactca tccgcatcag
     1861 cagcccgcgc cagacccgga gttacagcta cagcaccacc ggcaggctga ccggcgttca
     1921 caccaccgca gcgaatctgg atatccgcat cccgtatgcc acagacccgg cggggaaccg
     1981 cctgcccgac ccggagctgc acccggacag caccctcagc atgtggccgg ataaccgtat
     2041 cgcccgtgac gcgcactatc tttaccggta tgaccatcac ggcagactga cagagaaaac
     2101 cgacctcatc ccggaagggg ttatccgcac ggatgatgag cgcacccacc ggtaccatta
     2161 cgacagtcag caccggctgg tgcactacac gcggacacaa tatgcagagc cgctggtcga
     2221 aagtcgctat ctttacgacc cgctgggccg cagggtggca aaacgggtat ggcggcgtga
     2281 acgggacctg acgggctgga tgtcgctgtc acggaaaccg caagtgacct ggtacggctg
     2341 ggacggcgac cggctgacca caatacagaa cgacagaacc cgcatccaga cgatttatca
     2401 gccggggagc ttcacgccac tcatcagggt cgaaactgcc accggtgagc tggcgaaaac
     2461 gcagcgccgc agcctggcgg atgcccttca gcagtccggc ggcgaagacg gtggcagtgt
     2521 ggtgttcccg ccggtgctgg tgcagatgct cgaccggctg gaaagtgaaa tcctggctga
     2581 ccgggtgagt gaggaaagcc gccgc
//
LOCUS       DAKISA010000248         2579 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.252, whole genome shotgun sequence.
ACCESSION   DAKISA010000248 DAKISA010000000
VERSION     DAKISA010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.252"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            44..976
                     /locus_tag="OVW45_004476"
     CDS             44..976
                     /locus_tag="OVW45_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCV5528740.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(1017..2447)
                     /locus_tag="OVW45_004477"
     CDS             complement(1017..2447)
                     /locus_tag="OVW45_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCV5528741.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAVHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKLLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT          689 a          569 c          526 g          795 t
ORIGIN      
        1 ttctgctaaa atctgcgcct tgcgcttctt tagcgcactg tctatggcta accttttgaa
       61 taaattcatt atgacgagaa tactcgctgc gataaccctt ttgctgagta tcgtattgac
      121 cattttggtc actatttttt gttccgtccc gatcatcatt gccgggattg taaaactatt
      181 gctacctgtg ccagtcattt ggcgaaaggt ttcgcgtttt tgtgatttta tgatgtattg
      241 ctggtgtgaa ggtctggcgg tattactgca tctgaaccca cacctgcaat gggaagttca
      301 cgggctggaa gggttaagta agaagaactg gtatctgctt atttgtaacc accgtagttg
      361 ggcagatatt gtcgtactgt gcgtgctgtt tcgtaagcac attccgatga ataaatattt
      421 cctcaagcaa cagctggcct gggtgccatt tcttggcctg gcgtgctggg cgctggatat
      481 gccgtttatg aagcgttatt cccgcgctta tttattacgt catccggagc gacgtggtaa
      541 agatgttgaa accactcggc gttcttgtga aaagtttcgc ctgcatccca ccaccattgt
      601 caatttcgtt gaaggctccc gtttcacgca agaaaaacat cagcaaaccc actccacttt
      661 tcaaaacttg ttgccaccaa aggcggcagg cattgcgatg gcgctaaatg tactgggtaa
      721 acaattcgat aaactgctta atgttacgct gtgttatccg gacaataacc gtcagccgtt
      781 cttcgatatg ttaagcggta aactgacgcg gattgtcgta catgtggatt tacagcctat
      841 tgccgatgag ttacatggcg actacatcaa tgacaaaagc tttaagcgcc attttcagca
      901 atggctgaac tcattgtggc aggaaaaaga ccggcttctg acgtctctca tgtcatcaca
      961 gcgtcagaat aagtaagttt tactcgcctc tgaatgagca gaggcgagtg agtattttaa
     1021 tgaacaaaac gtccggcacg agacataaat tcttcttcgc tcatctcctg tccgttaaac
     1081 acaactttac ccggtgtata acgcagctgc agcgaggcgg tattgttgtc aatcgtgatt
     1141 aactgaaaca tctgacccaa tgtcatcatc ccgctgattt gtttatcagc ctgcttttgc
     1201 gctttttccg catccattcc ttcagataaa ttaagctgtt ttgcggtttc tgtgacgaca
     1261 ttaagcggta acttcacatc aaaattgagc aatttgatat ctttgtttgt ggatgatgaa
     1321 gatttggctg ggtcggcaat actgatatcc agattggcat ttagttcgcc gagtgcgttc
     1381 ttccatctta ccggttgttt aatggtcggc tcactttttt gtaataacgg taaatactct
     1441 ttgaacaatg cagcattcac ttcttgcaga gcaacttcat cgtttgctaa ctcagggtgt
     1501 acagcaagct gcttctgcat agcaatgtta tactggatga taaattggcg cactgctgag
     1561 ggatcaatag attcagcaat caatgaccac tcaccactac cgagttgctg attccctttt
     1621 gttaacttat caagttcgta ggtcagattg acattgatat aatctttgtc tttaacgcga
     1681 tccagcatgg tttttgcaac gaaagcatcg atttgggcaa cgtcttcccc gtgattgata
     1741 atgttaatct gattaaattt cgactcgctt tcaccaaccg gaaatttctt ttcttctaat
     1801 cgcgccagag aatcaatggt aaaacttttt gccgttactt tgttcattgg tgataattgc
     1861 agaacaatat cttcgacttt gccttcaata tccaggttag acaatgaact accatcacca
     1921 ttaaaagtaa actggccctc accaaaagaa aactttccat actctgcagg ggcaacatct
     1981 aatgtcgtag agaatgagcc accaaacgcg aatcgcaatg tcgcttccac gggcgattta
     2041 tttttcgcgg cgataaatag cggttgtgtc agttcattat tcactaagtt cacttttgcc
     2101 gctgccagtg ctgggatgac attaccatgc attaacattg tgatgggcaa cggaccgtgc
     2161 tcaacatcca catcaaatac aactttctgg cctggcttga tattgagatc gggtgcaccg
     2221 ttatcgaatg tcatttgcat ttgaaaacga gaattaaaaa aacctttgtc aaaattttta
     2281 taacttaacg tcatatcgta ggcatgttca cctttctttt tagcatcgtt aaaatcttta
     2341 ataaactttt cgacaccagg ctgaatttgc gtacctgtgt accatgtgcc accaccccag
     2401 attacggcta acgcaacaat aacacctgta gctgacttac gtatcatata aatattccct
     2461 gataaaacag caaaacttta aatatccgct tatgctgaaa taatgtccag cggcaggacg
     2521 cattatcaga atgtatttat atttattaaa taataaaaaa aaccgtgaat attcacggg
//
LOCUS       DAKISA010000249         2562 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.253, whole genome shotgun sequence.
ACCESSION   DAKISA010000249 DAKISA010000000
VERSION     DAKISA010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.253"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..805)
                     /locus_tag="OVW45_004478"
     CDS             complement(<1..805)
                     /locus_tag="OVW45_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HCV5528742.1"
                     /translation="MKKFFRHFFLILCLSCYTASAGTDDNVGYIVGNSYGVGPSVQKW
                     RETGPNGDATVIFRYATSTNNLVFYKPTQLGPTGVKLQWSQLDTASGGGFLYCNRSDS
                     TSGSAMRIENAMVDSGKMYGSHKLFNTSVPGLYYTLLISNMWSAYGTVTNVSSPGIYI
                     GDSAEQYFSWYNPSEDVLYWSCNNANSTRKYWAVGGIYQTLTIEFYTDTNFDPTVTQQ
                     IKLSSSSNYLYSFKAYGAGQGINEHSYFIKIDFDLLNVKLTNPTCFTAML"
     gene            complement(858..1454)
                     /locus_tag="OVW45_004479"
     CDS             complement(858..1454)
                     /locus_tag="OVW45_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCV5528743.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            complement(1481..2083)
                     /gene="yadL"
                     /locus_tag="OVW45_004480"
     CDS             complement(1481..2083)
                     /gene="yadL"
                     /locus_tag="OVW45_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HCV5528744.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGLANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            complement(2098..>2562)
                     /locus_tag="OVW45_004481"
     CDS             complement(2098..>2562)
                     /locus_tag="OVW45_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCV5528745.1"
                     /translation="ATTCNITLTGNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADF
                     KLVASGCSSGISWIDTTLTGNASSSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATF
                     LKPNSAEKIRWSTDEMQPDKGLEMTVALRETDAGQGVPGNFRALATFNFIYQ"
BASE COUNT          714 a          553 c          513 g          782 t
ORIGIN      
        1 taagcatagc ggtaaaacaa gtgggattag tcaacttgac attgagcaga tcgaaatcga
       61 ttttgataaa ataactatgc tcatttatac cttggcctgc gccataggct ttaaatgaat
      121 acagataatt tgatgagctg gataatttga tctgttgagt tactgttgga tcaaagtttg
      181 tatctgtata gaattcaatt gtaagggtct gataaatacc acctacagcc cagtatttac
      241 gggtgctatt cgcattattg caactccaat ataacacgtc ttcgcttgga ttataccacg
      301 aaaaatattg ttctgcagag tcaccaatgt atatcccagg tgaactaacg ttagttacgg
      361 taccgtaagc tgaccacatg tttgaaatta ataatgtgta atacaaacca ggaactgatg
      421 tattaaataa tttatgggag ccatacattt tacctgagtc aaccattgca ttttcaatac
      481 gcattgcgct accacttgtg ctatcacttc tgttgcaata aagaaaacca ccaccagaag
      541 cggtatctaa ctgactccac tgtaatttta cacctgtagg tccaagctgc gtcggtttgt
      601 agaaaactaa attatttgta cttgttgcat atctgaatat gacagtagca tcgccattcg
      661 gccccgtttc tctccatttt tgaactgacg gaccaacgcc gtaactattc ccaacaatat
      721 atcctacgtt atcatcagtg ccagcactcg cggtataaca actcaagcac agaattaaaa
      781 aaaaatgtct gaagaatttc ttcattatca ctccaccatc tactgctgta acagtcgcag
      841 ttatttgttt tgatctatta ttcgtagtac acatcaacta atatatgact cattacttgg
      901 ccagatatga catccagcgc gggatcgatt ttttgcattc tggcataaaa tttccatgaa
      961 gaattatgca gggcagaagt cattactgga tacacggaac gagaatttcc tgctgaacta
     1021 ataacattga atatattggt cgggtcctga atggaaaaga gaacgacacc gacattttta
     1081 gccccggttg cgacttcatt ggcaaatatt tgccttgaac ctgcagccat gacaccgcta
     1141 agggctctaa aatcaatatg taactgatta agcacatcgc cagttccctg taatgcacag
     1201 tctgatactg tgacagtgaa ctcttttcca cctggttgat agctttcagg agtaacatta
     1261 tctgaaaaat atcccacccc aacgacgcca agatcaatat taccctggtt actgattccg
     1321 cttttgcaag tgctgttttt tacattggca acaaggtcaa tatcctgccc agcgatggct
     1381 ggataggtaa tgggaaactg tagtgacaga aacagaatta ttctcttcat cagcttacgt
     1441 tgagtgggat gcatatatag ttcctggatc tgtcagtctg ttagttatag gttactgtaa
     1501 atgtggcagg tgcagaaaat ttacccgcag tgacattgct tacggtttta tttttttcga
     1561 cgaccagtcg agcactcatc ggataataaa caaccgcgtt ggctgcattg gagatagtga
     1621 cctcttgaga tgctggtgtc acacaactaa attgtgttgc gctccctgtt gccggagttt
     1681 cggtgctcca gacttcaacg gcgacggcac ttgctttatc tgcggctgtg gatccatttg
     1741 cgagccccgc accgtcatta gcagtacctt cgcatgtggc tcgcttggtt aattttattt
     1801 gcgctttttt attggggata cccgcacagt ctttgaattt gagtttgaat gttttaactt
     1861 tggtcttggc attgatttca gaaatatata catcgccaaa gtcaacaaca gaaatgtcct
     1921 gatcagagtc atttactaat gtagcggtac aagtacctat ttctacggta gtaatcacgg
     1981 tcagcccaat gctgtctgtt gccgcataag agggtacgct gaacaatgag gcagccaaaa
     2041 cgacactgct ggataatcca taacgtaaat ttttaaatgt catcttaatt atccttctta
     2101 ttgatagatg aaattaaacg ttgccagtgc acgaaaattc cccggtacgc cttgccctgc
     2161 atctgtttca cgtagcgcaa cggtcatttc aagaccctta tcgggctgca tctcgtctgt
     2221 gctccagcgt atcttttccg cactgttagg tttaaggaaa gtggcatcat cagtagtccg
     2281 ttttttgaaa cccataccga tattacttgt cgtcgaagat gaatcaccag actgcggtat
     2341 aataagctta ggtgagcttg atgatgcatt tccggtcaga gtggtatcaa tccaactgat
     2401 gccactgctg cacccactag caaccagttt aaaatcagcc tgagattctg tcgttttatt
     2461 cgcgatctta tccagaccca tcttagggat tctcaatgtg tagttattat tgccatcatt
     2521 cgtgacgtta ttaccagtaa gtgtgatgtt acaggttgtc gc
//
LOCUS       DAKISA010000250         2557 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.254, whole genome shotgun sequence.
ACCESSION   DAKISA010000250 DAKISA010000000
VERSION     DAKISA010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.254"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(58..261)
                     /gene="tatE"
                     /locus_tag="OVW45_004482"
     CDS             complement(58..261)
                     /gene="tatE"
                     /locus_tag="OVW45_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCV5528746.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(390..1178)
                     /locus_tag="OVW45_004483"
     CDS             complement(390..1178)
                     /locus_tag="OVW45_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCV5528747.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            1271..1654
                     /gene="crcB"
                     /locus_tag="OVW45_004484"
     CDS             1271..1654
                     /gene="crcB"
                     /locus_tag="OVW45_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCV5528748.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="OVW45_004485"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="OVW45_004485"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCV5528749.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(2092..>2557)
                     /gene="pagP"
                     /locus_tag="OVW45_004486"
     CDS             complement(2092..>2557)
                     /gene="pagP"
                     /locus_tag="OVW45_004486"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCV5528750.1"
                     /translation="TFRENIAQTWQQPEHYDLYIPAITWHARFAYDKEKTDRYNERPW
                     GGGFGQSRWDEKGNWHGLYAMAFKDSWNKWEPIAGYGWESTWRPLADENFHLGLGFTA
                     GVTARDNWNYIPLPVLLPLASVGYGPVTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT          652 a          659 c          581 g          665 t
ORIGIN      
        1 tttgaagcgg tttttttgta tcgaacaaga tattgaggga gcgtcctgct cgccacgtca
       61 ctctttatga gagagctttt cagcctgaag atcaacgtct gcgccttttt tcgccgcagc
      121 atcgtcatca ttcatcgcct tcttgaaccc tttaatggcc gctccaaggt ctccgcccag
      181 cgtacgtaac ttcttagtcc caaacagcag aacgaccagc gccgcaacta ccagcagttt
      241 ggtaatacta atctccccca tagatacctt cttgacatat aaccggcaac agaacgcgcc
      301 ttaaaaacca gagaatatta acggtcattc gacgcgcgca cacaatagca atctgtaaca
      361 aggtgaatca agcgccgagt aaaaaaacat cataataatt gcggcggcgc aaagcgacgg
      421 ttgtttaaga cgggcagttg cgcgcgcacc tgacgcacac gttcgggcgt cacttccgcc
      481 ataatgagtg caggcatttc tgacgctgcc gcaatggtga cgccaaaggg atctataatc
      541 cggctttgcc cgatattttt gttcccgcac tcccccgccg ccaccatata acaggtggta
      601 tccagcgcac gagcggcaag caacgttgac cagtgatgct ctttgagcgg cccgcgaacc
      661 caggcggcag gaagtaccag gatttcagct ccctgtaatg cctgtgccag cgccagctct
      721 ggaaagcgta agtcataaca ggtcatcaga ccgaccttca tcccctccac ctcgagtaac
      781 ggagcgattt cattaccagc atcaacacgg cgtgattcct gaatggcaaa tgcatcatag
      841 agatgcaatt tggcataacg ggcgacgatg tttcctgcct gtagcgccac cagcatattc
      901 catgcgcgcc ccggcgttga aggaacatga atcgtcagaa ttgtcgtcat catgttacgt
      961 ttactttctc gccgtaaacg tccgaggaat tcgccttcca gcagctgtgc tgatttaacc
     1021 gatagatctg catcatgatc atcgcgcgcc agcaatgctt ccggcaggac gaacagcgat
     1081 acgtcgtttt ccgccgcctg cgccatcaac gaggcacaaa tctcagcgtt cttttcccac
     1141 acagatgtaa cagcaaactg tcctgcggca accagcattg ccacctcctg tcagagtcgt
     1201 aaaattcgcc aacgacgatt attcggcgtt acacttatca ctcatacaaa tcaaatagca
     1261 ggattttgca gtgttacaac ttcttttagc agtttttatt ggcggtggta cgggaagcgt
     1321 ggcgagatgg ctgttaagta tgcgatttaa cccgctgcat caggcgattc cgttggggac
     1381 gctggcagca aacctgattg gggcattcat cataggaatg ggattcgcct ggttcagcag
     1441 gatgacgaac attgatccag tgtggaaagt attaatcacc accggatttt gtggcggtct
     1501 aacaaccttc tcaacatttt cggcagaagt ggtgtttttg ttacaagagg gccgctttgg
     1561 ctgggcgtta ctgaacgttt tcgtcaacct tctggggtct tttgccatga ccgcactggc
     1621 tttctggctg ttttcggcct caaccgcaca ctaaagacca aaaaaaaccc gctgattaag
     1681 cgggttttga attcttgctg acgtatctta cagagcgatt acgtttgcag cagaagggcc
     1741 tttggcaccg ttagtgattt cgaactctac gcgctgacct tcagcaagag ttttaaaacc
     1801 attagtctgg attgcagaga agtgtacgaa cacgtctttg ctgccgtctt ccggagtaat
     1861 gaaaccgaat cctttggact cattaaacca cttaacgtta cctttaatct tagacatcaa
     1921 aattaccttt acatgaaaaa tagacacaaa tgctgtgtcg gttaccagta caccaattgt
     1981 ggtacgcttt gtccagtgca actttactaa aaagtgataa aagtcgcgtt atttttgatg
     2041 ttacttattt tagttattga ttttaaagaa gttactaaaa cttcatttgt ctcaaaactg
     2101 aaagcgcatc caggcaaagt acacattgcc attgttgtag gtacccggaa tgtaggtcat
     2161 ctgaaaagtc actgggccat aacccacgga ggccaatggc agtagaaccg ggagagggat
     2221 gtaattccag ttatcgcgtg ccgttacgcc agcggtgaat cccagaccta aatgaaaatt
     2281 ttcatccgcc agcggtcgcc aggtactttc ccatccgtat ccggcaatcg gttcccattt
     2341 gttccacgag tccttaaatg ccatggcata caggccatgc cagtttcctt tttcatccca
     2401 tcgcgactga ccaaaaccgc caccccacgg tcgctcgtta tagcgatcgg ttttttcttt
     2461 gtcgtaagcg aaacgtgcat gccaggtgat ggcaggaata tataaatcat aatgttcagg
     2521 ctgttgccag gtttgtgcaa tattttctct aaacgtt
//
LOCUS       DAKISA010000251         2540 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.255, whole genome shotgun sequence.
ACCESSION   DAKISA010000251 DAKISA010000000
VERSION     DAKISA010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.255"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(107..1525)
                     /gene="phrB"
                     /locus_tag="OVW45_004487"
     CDS             complement(107..1525)
                     /gene="phrB"
                     /locus_tag="OVW45_004487"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCV5528751.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGVGSVWLNEL
                     IWREFYRHLITYHPSLCKHRPIIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDLEGEFIRQWLPELRNVPGKSVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(1522..2031)
                     /locus_tag="OVW45_004488"
     CDS             complement(1522..2031)
                     /locus_tag="OVW45_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCV5528752.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYHDAWLSGQRYFELWPRV
                     INLRHSGVL"
BASE COUNT          642 a          659 c          580 g          659 t
ORIGIN      
        1 caggcctacg tatttcctgc aatttattga atttgcacaa atttgtaggc tggataaggc
       61 gttcacgccg catccggcat ctgggctcga ttgcctgacg cgtctgttat ttccccttcc
      121 gcgccgcctc atacgctgcc aacgtttgta ctctcgcctc tttgtgctcg actatcggtt
      181 gcggataatc cagcttcaca cctgctttct gcgcccactt ccacggctca tgcaccgatt
      241 tccccggtac attgcgtagt tccggtagcc actggcggat aaactcacct tcgagatcaa
      301 atttctcgcc ctgggttgtc gggttgaaaa tacgaaaata cggcgctgca tcggttccgg
      361 ttgaagcggc ccactgccag ccaccgttat tggctgccaa atcaccatca atcagctgcg
      421 acatgaaata tcgctcgcct tcgcgccagt cgatcaataa atctttcacc agaaaactgg
      481 ctgtaatcat ccgtagcctg ttatgcatcc agccagtgct gttaagctga cgcatagcgg
      541 catcaacaat cgggtatccc gttttgcctt cctgccaggc ctgtaaatgt gcgggattgc
      601 tctgccactg tacacgatcc gtccaggcaa taattggacg atgtttacac aacgaggggt
      661 gatacgttat caggtgacgg taaaactcgc gccagatcag ctcattaagc cagacactac
      721 cgaccccacc gtccagcgcc tgcggctgtt cagccaacaa gcgatgcaag cactggcgag
      781 gcgataaccc gcccgttgcc aggctggccg acaaacggct ggtgccttcc actgccggaa
      841 aatctcgttg ttgctcatat tctccggcac cgttctggca aaactggcgt aattgcgcaa
      901 tcgccgcttt ttcttccacc ggaaaatgcg cagtatcgaa agactgacga ggataattca
      961 gcgtaatgga tggcgagggc tctatcgatc cgctactacg aacttttggc gcagcgacgc
     1021 actccggcat cccttcccgc agccgtttca gccaggcatt cttaaaaggc gtaaagactt
     1081 tgtacatctc gtgattaccg gtcatcaccg cgccaggcgg caggatcacg ctgtcatcaa
     1141 atccttcaca caccacgtta cgcagcgctc tttcaacttc cacatcccgc gcccgctcat
     1201 tcacttcata ctgatagtta taaaacaggt gggtaacgct gttttccgcg cacacctgtt
     1261 taactatttc gacactggcg acaaagtcat ccacttcacg gaacaataaa ggaatacctt
     1321 tttccgcaag cgctatttgt agcccattca gttgagcatt gataagttca gcctgacgcg
     1381 gcgacatgtt atgcgtcgcc cactggcgtg gtgtagcgat atacaacgcc agcacgcgtg
     1441 cagacgaatt gcggcaggcg gcagccagtg cgagattatc gtgcagacgt aaatcttggc
     1501 gaaaccagac cagatgggta gtcataaaac tcctgaatgg cgcaagttaa tcacacgcgg
     1561 ccaaagttcg aaataacgct gccctgaaag ccaggcgtca tgataaagcg ccatatagtg
     1621 tttgatacgc atcagcggcg caagaagtgg ttgctcgcca cggcgataac tgtcgataag
     1681 cgcagccagc tgctggcgtt ctgtggaatc aatatgcggg cgaaaataac cctgaacgtg
     1741 catcaaaaca ttggtgtgat cgcgcgggtt ggcggggtga gaaagcaaaa caactacgcg
     1801 ttggcggtac tggttgtaat agtcatccag attttgccac tggtgaatat cagccacgaa
     1861 cgggccaagt ttgcggtact ccggctgaga atgcgccaga aagacgagtt tatagcggct
     1921 gtgataatcg agaagcgccc cgcgcgttaa gccatgttct ttcagtcgat gtaactcatg
     1981 aagggcaaag atacggatta gggtgctgtc atcaaatcgt tgtagattca ttactgctcc
     2041 tgaacggtat aaaggagtaa gtgtagacga tggtaaatga attttatggg gcagagtttt
     2101 tgcggggaag tattttgttg gtaaaaatat tatcaacttc atctgagtga gaattattct
     2161 actatttccc gagctgcatt cattttttgt attttttatt tttagaaaat aacagttctc
     2221 aaagcttaat tacctcacta ttaattgtat ttcaaactaa caatcgcact ccattttctc
     2281 tttttaaaaa acataaacac tgttaaaaca aatacttatt tcgttatctt aaatgctatt
     2341 tctacatcac tcgtccttat aatcaaatta ttcttattca tagctatttt tgcaggttta
     2401 aaatagcggc tttcaccaaa gtcagccatg aaaattagac tacaagtcag ggcaagatta
     2461 cgaaagcccg ctccccgcaa ggactgacgc cagatagttt ctgtccatgg ctgcttttcg
     2521 catcttacgt cttaaccctg
//
LOCUS       DAKISA010000252         2421 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.256, whole genome shotgun sequence.
ACCESSION   DAKISA010000252 DAKISA010000000
VERSION     DAKISA010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.256"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            704..1549
                     /locus_tag="OVW45_004489"
     CDS             704..1549
                     /locus_tag="OVW45_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCV5528753.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGIATPKSFTAHNRAQAVFLC
                     VMHSHIQIMVGRAGQSQDWPGSVMTGCSNPVRLTTHEIATSGGELIKFIAEAAIMATT
                     LSHPCVTVENGRAVTTSVAVAEFFHKRHDNVLRAIANIECSDKFTALNFEASEYTDSI
                     GRKLPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMIPG
                     DGRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVEQL
                     LKIHC"
     gene            1560..1748
                     /gene="dicB"
                     /locus_tag="OVW45_004490"
                     /gene_synonym="ftsT"
     CDS             1560..1748
                     /gene="dicB"
                     /locus_tag="OVW45_004490"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HCV5528754.1"
                     /translation="METLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKHERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            1745..1936
                     /locus_tag="OVW45_004491"
     CDS             1745..1936
                     /locus_tag="OVW45_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HCV5528755.1"
                     /translation="MNTVFALVLTVFLVSGEPVDMVTGVYSSMKECMTAAAKQKIPGD
                     CYPVDKVIHHDNNEIPAGL"
     gene            2021..2296
                     /locus_tag="OVW45_004492"
     CDS             2021..2296
                     /locus_tag="OVW45_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528756.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
BASE COUNT          697 a          527 c          566 g          631 t
ORIGIN      
        1 gcggggtata aataccatgc gcattgactg aattcgcaaa caaaaacaga cgcgcgatac
       61 ctggatagtc ggtctgcata gtcagatatc tggctgcgaa tttgataaat atcaccgccc
      121 ttttcggaaa cataagttcc gtccgggagt tgattatacg aaccatctcc atgggggatc
      181 gttctcctga atcctagtga gagcatatat ttatgaagcc cttcgtaatc ctctggctca
      241 gcattatata gttctactct ggcgagatac gttggcatat tcatttcctt actggttgtg
      301 tgagaacttc agtaaagata ccaccaaagc ccggaggtgg tgaaataaaa ccgggcacaa
      361 cacgaaggcg catttccggt atccataaag agtcggtctt gtctgttaaa tttaaatggt
      421 gggagtgcgc ctccggttgt aaataacgac attgctgtgt gaagtaccag ttggcggcat
      481 cggtttaatt gctggctgat gtccgccctt tttaaagtga attttgtgat gcggtgaatg
      541 cggctcagcg cacgcggcac agttaaaaag cgattagttc ccccgtatcg ggtggttatg
      601 ggtttccctg tatccggcgt taattgttaa ctggttaacg tcacctggag gcaccaggca
      661 ccgcatcaac aaagttcact tcggtgatga aaggaaagag aaaatgttga atgtagctat
      721 tgaaaaccag aacgggtgga attatagtgc acctgcacct cataaaacgg gtgccgggat
      781 tgctaccccg aagtcattca cggcgcataa ccgcgctcag gcggtttttt tatgcgtaat
      841 gcacagccac attcaaatta tggtggggcg tgcggggcag tcgcaagact ggccgggttc
      901 cgtgatgacc ggttgtagca accctgtacg tctcaccacc catgagattg ctacctccgg
      961 tggtgagtta atcaaattca tcgcggaggc tgccatcatg gctactaccc tttctcaccc
     1021 ttgtgtaacc gtagaaaacg gtcgcgctgt cactacgtct gttgcggttg cagagttctt
     1081 ccacaaacga cacgacaatg tgttacgtgc catcgcaaat attgaatgct cagataaatt
     1141 tactgccctc aattttgaag cgagcgaata caccgactca atcgggcgca aactcccaat
     1201 gtaccaaatc accaaaaacg gcttcgtttt cctggttatg ggcttcactg gcaaaaaagc
     1261 cgctgcattc aaagaagcct acattgctga gttcgaccgc atggaggccg aactgcgcca
     1321 gaataacacc ccacctgctg acaagatgat tccgggtgat ggtcgcactc tggttgttca
     1381 cttcgacaaa ttcggcaatg tcgaattcac cgaaaccgtt cctgatggcg cactggtctg
     1441 taccctggag actttccggt tttatctgga aaaacaggga tggactcttg ttaaccgggg
     1501 cgcaattaaa aatatgactg tggaacaatt actcaaaatt cattgttgag gacgcaataa
     1561 tggagacgtt attaccaaac gttaatacgt ctgaaggttg ttttgaaatt ggtgtcacta
     1621 tcagtaaccc tgtatttact gaagatgcca ttaacaagag aaaacacgaa cgggagctat
     1681 taaataaaat atgcattgtt tcaatgctgg ctcgtttacg tctgatgcca aaaggatgtg
     1741 cacaatgaat acagtatttg cactcgttct gacggttttt cttgtttccg gtgaaccggt
     1801 tgacatggtt actggcgtat acagctcaat gaaagaatgt atgactgccg cagcgaaaca
     1861 gaaaattccc ggtgactgtt atccggtcga taaagttatt caccatgata ataacgaaat
     1921 cccggcaggt ctttaaaaca gttccgtaat aaatatccgg tttcattctt atatgccagc
     1981 aatggcaggg gttcgctcaa ccttaattaa ggagaaaaac atgattacca gttatgaagc
     2041 cactgttgta actactgatg acattgttca cgaggttaat ctggaaggaa agcgtattgg
     2101 ctacgtgatt aagacagaaa ataaagaaac cccattcact gtggttgata tcgacggtcc
     2161 atcaggcaac gttaaaacac ttaacgatgg tgtaaaaaaa atgtgtctgg tgcacatagg
     2221 aaagaatctg cccgcagaaa aaaaagccga atttctggca actctgattg caatgaaatt
     2281 aaaaggtgaa atctgaaaaa aagaaagcct gcacactgtg caggcctgag tgaagaacct
     2341 gggacattta ttcatcactc gcagtaattt taatctgagt tgaggttaaa aaacaatgag
     2401 caccgataaa caagtttacc c
//
LOCUS       DAKISA010000253         2384 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.257, whole genome shotgun sequence.
ACCESSION   DAKISA010000253 DAKISA010000000
VERSION     DAKISA010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.257"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(502..645)
                     /locus_tag="OVW45_004493"
                     /pseudo
     CDS             complement(502..645)
                     /locus_tag="OVW45_004493"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(649..1467)
                     /locus_tag="OVW45_004494"
     CDS             complement(649..1467)
                     /locus_tag="OVW45_004494"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCV5528757.1"
                     /translation="MSHIRPDFPEPYGLPVDGLRGKTSIFSHPVYLFLREFSLQDSRG
                     GSLSKKAESLSSGKITIGTKEKVIIINELRQCHPLSQLLVIADLPRSTFYYHVKRLNA
                     PDPYQLVKQVILRIYHQHKGRYGYRRISSLIRRKKYRAYRGEQGRTCNNLLKRQFYAD
                     RPNQKWVTDVTEFKVDGRKLYLSPIMDLYNGEIVSYNLTERPLASMVKSMLLDAVEQL
                     NKDDKPLLHSDQGWQYQMPRWQRWLSDNGITQSMSRRGNCLDNAAMESFFSTLK"
     gene            1434..1709
                     /locus_tag="OVW45_004495"
     CDS             1434..1709
                     /locus_tag="OVW45_004495"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCV5528758.1"
                     /translation="MVLESQGEYDSQWAVICSITPKIGCTPETLRVWVRQHERDTRGG
                     DGGLTTAERQRLKELERENRELRCSNDILRQASAYFAKAEFDRLWKK"
     misc_feature    1664..1780
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            1709..1858
                     /locus_tag="OVW45_004496"
     CDS             1709..1858
                     /locus_tag="OVW45_004496"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528759.1"
                     /translation="MPLLDKLRGQYGVGPVCSELHIAPSTYYHCQQQDIILINAVPVR
                     STMTG"
     gene            1795..2193
                     /locus_tag="OVW45_004497"
     CDS             1795..2193
                     /locus_tag="OVW45_004497"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCV5528760.1"
                     /translation="MSATGHHPDKRSARAQHDDWLKREIQRVYDENHQVYGVRKVWRQ
                     LLREGIRVARCTVARLMAVMGLVGVLRGKKVRTTVSRKTVAAGDRVNRQFVAERPDQL
                     WVADFTYVSTWQGFVYVAFIIDVFAGYIVW"
     gene            2209..>2384
                     /locus_tag="OVW45_004498"
     CDS             2209..>2384
                     /locus_tag="OVW45_004498"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCV5528761.1"
                     /translation="METTFVLDALEQALWARRPSGTIHYSDKGSQYVSLAYKARLKEA
                     KLLASTGSTGDSYD"
BASE COUNT          624 a          533 c          565 g          662 t
ORIGIN      
        1 tatgacctta cgactgcgcc tgcaggatca gcaggaagaa acatccgtga cattatttca
       61 gggaaaaatg agtaccgacg gaaataacta ttacatcatc aaaggtaaag taagctcagt
      121 acttctgtta ttaaaaatgc tgcagattaa tcacgcctga tctctgtccc ggtattttag
      181 cccgaggtat attctgaaat atcattcatc atgaccggcg tttatacaaa cgccggtcat
      241 tcgctaacag cttcaccgtt ccttccccca tccaggttcc gcatgagcgt gttgtaatgt
      301 tcataagcgt attatcagga ttgtctggaa aattcccgat actttggact attactcctg
      361 aacaattcga aattcagcag attaaaggta agcgttaacg gaggtaagcg taaaccgact
      421 gccgtatgta gccattaagc cagtattggt aacgtaggtg cccacctttt caaatgatct
      481 gcgccccaaa tcttggacat cctatgaaaa atgtttaagt cgatattgaa ccggactcat
      541 gccatcgagt ttctctttaa tgcgatggtg attgtagtat tcaatgtaat caattatatc
      601 ttgctttaga tgatgtaact tattgtattt atttaggtag taacattctt attttaaagt
      661 actaaaaaag ctttccattg ctgcattatc taagcagtta ccacgtcggg acatgctttg
      721 tgttatgcca ttatcactca accaacgttg ccaacgaggc atctgatatt gccacccctg
      781 atcactatgc aacaagggct tatcgtcttt attgagttgt tctaccgcat ctaatagcat
      841 gctctttacc attgatgcta atgggcgctc agtaaggtta tatgagacta tttctccatt
      901 atacaggtcc atgattggtg ataaatagag cttcctgcca tcgaccttaa actctgtgac
      961 atctgttacc cacttttgat ttgggcggtc agcataaaat tgtctcttca gtaaattatt
     1021 acaagttcgc ccctgttctc cacgataggc acggtacttc ttgcgcctga taagtgagct
     1081 gatacgtcga taaccataac gccctttatg ctgatggtat attctcaata ttacttgttt
     1141 aacaagttga tatggatctg gcgcattaag gcgtttcacg tgatagtaaa atgtactacg
     1201 tggcaaatcc gcgataacca gtaattgtga taatggatga cattgcctta actcattaat
     1261 aatgattact ttttcttttg ttccaatcgt aatttttcct gagctaaggc tttcagcttt
     1321 tttagataat gaaccgcccc gggaatcctg gagactaaac tccctgagaa agaggtaaac
     1381 aggatgacta aaaatactcg ttttcccccg aagtccgtca acgggcagtc cgtatggttc
     1441 tggaaagtca gggcgaatat gactcacaat gggcggtaat ttgttccatt actccaaaga
     1501 ttggctgtac accggagact ctgcgtgtct gggttcgcca gcatgagcgg gataccaggg
     1561 gcggtgatgg agggctcacc accgctgaac gtcagcgtct gaaagagctg gagcgtgaga
     1621 atcgtgaact gcgctgcagt aacgatatcc ttcgccaggc ttccgcttat tttgcgaagg
     1681 cggagttcga ccgcctctgg aaaaaataat gccactgctg gataagctgc gtgggcagta
     1741 cggggtcgga ccggtatgca gtgaactgca tattgccccg tcaacgtatt accactgtca
     1801 gcaacaggac atcatcctga taaacgcagt gcccgtgcgc agcacgatga ctggctgaag
     1861 agagagatac agcgcgtata cgatgaaaat catcaggtgt acggtgtgcg taaagtctgg
     1921 cgccagttgt tacgggaagg aatcagggtg gccagatgta cagtggcgcg cctcatggcg
     1981 gttatgggac ttgtcggtgt tctccggggt aaaaaggtcc gcactaccgt cagccggaaa
     2041 accgttgccg caggtgaccg cgtaaaccgt cagttcgtgg cagaacgtcc tgaccagctg
     2101 tgggtggctg attttactta cgtcagcaca tggcagggct tcgtctatgt ggcgttcatc
     2161 attgatgtgt ttgccggata catcgtgtgg tgatgagtct catcgtctat ggaaacgaca
     2221 ttcgtgctgg atgcactgga gcaggcgttg tgggcccgtc gtccgtctgg caccatccat
     2281 tacagcgata aaggctctca gtatgtgtca ctggcctata aggcgcgact aaaagaagca
     2341 aaactgctgg catcaacagg gagtacaggc gactcgtatg acaa
//
LOCUS       DAKISA010000254         2365 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.258, whole genome shotgun sequence.
ACCESSION   DAKISA010000254 DAKISA010000000
VERSION     DAKISA010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.258"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(190..402)
                     /locus_tag="OVW45_004499"
     CDS             complement(190..402)
                     /locus_tag="OVW45_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCV5528762.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            complement(531..1091)
                     /locus_tag="OVW45_004500"
     CDS             complement(531..1091)
                     /locus_tag="OVW45_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCV5528763.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            complement(1146..1893)
                     /gene="traX"
                     /locus_tag="OVW45_004501"
                     /pseudo
     CDS             complement(1146..1893)
                     /gene="traX"
                     /locus_tag="OVW45_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
     gene            complement(1913..>2365)
                     /locus_tag="OVW45_004502"
     CDS             complement(1913..>2365)
                     /locus_tag="OVW45_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HCV5528764.1"
                     /translation="IAGEGRPWNPGAITDGRVWGDIPDNSVQPGAGNGEPVTAEVLAQ
                     RQAEEAIRRETERRADEIVRKMAENKPDLPDGKTELAVREIAGQERDRAAITEREAAL
                     PESVLRESQREREAVREVARENLLQERLQQMERDMVRDLQKEKTPGGD"
BASE COUNT          473 a          749 c          572 g          571 t
ORIGIN      
        1 gcacacactg aataacacct tattttgtga aattaatcac aaaatataaa atcacgactt
       61 aatacggaaa aaactctgtg ctactcagaa ctgtccggtc atactgcccg accggatatt
      121 aaaaaaagca aatctcataa actcctttca gccttctgac tcctggagta atgaaggctg
      181 cttttttcgt tacacccttc cccggcagcg ccggtggcag tgttcagccc tttttcgggc
      241 aaactgctgc cagtctgtcc tgggcgcaac acaggcagcc cgtcgccatg cttctgccgc
      301 ttccatccag cgtccactga cttccagaat aacggcccgc ccgccggcac gctggtaacg
      361 ccagacctgg ccgttccgtg aactgtttct gaatccattc attttcttgt ccccctttag
      421 ttgtcagtat tattgtgata aataattcag tggactggct tcctttcgga agcggaagtt
      481 atttcctttt aagccttatt tccctgcctc ctctctgatg gaggcttttt ttatttctca
      541 tcaagcacgg cctgaagttc tgcctttatc cggttctggc ggcggatttt atccagacgc
      601 gctgccgcat acgcttcctc ctcctgagaa atatgctccg tcacataccc ttccgtgtca
      661 taccggcagg caccggcttt catggcacac agatagcttt ctgaacgggt gatggccttc
      721 agcgccctgc gcagtttttt atgcgagagc gggatattcc gctgcgccac gtcttccagt
      781 aacacgtccc ggataccgca ggccagaagc cggggggtgt caccgtcaaa taatcccggc
      841 caccagggtt tcagggtatt cacggcctca tccagcgtgg gcaggttcag ataaatggac
      901 agtgcctgtc tggcctgcgc tttttttgcc gccagctctg cttcccgggc tgctttctcg
      961 gccagcttct gctttttcac cttccacttt ggtggcgtgg tgacattgat gatggttttc
     1021 cggctgcgga caggcgtctc cccttccgtt ttccgcttca gtgtcagtac cggtcgcttc
     1081 tgctctgtca tgctgtgttc tgcctctgac gaatcatcct gttacggtca aagataccac
     1141 acccgtcaca gcgccagaac gcccagcaca gccagatgac aggcgtagaa taccgggaaa
     1201 aaatccccgg gccagaaacg gggtaatgat tttcctgcac acgacaccag accaacggtc
     1261 agcaccgtca tcacaagacc ggccaccgcc gctgcatcac tggtggcaag gttaagcgcc
     1321 ggaataacag ccagcaggca ggcgaccagc gccagacgtt ccgctctgtc ttccgcccgg
     1381 tacagtcggt ggctgactgc cagcatcagc agcccggcaa tgccgtaact ggtgccggac
     1441 aaaggccccc aacagggcca tcagaagaat ggccgctgct gtacgccacc cactgcgcgt
     1501 ttcacaccac gtcagcacct gtgccgccac tgcaaaggca aacaggatat tcccctcata
     1561 ccagggaaag cctgccagat aatatgcgaa ctgggcaata attccccatc cccacagccg
     1621 gttaatggcc ggttgccgga tatgcgcatg acgggacaga ttcagccccc acaccagggc
     1681 aaaaagcgga aaggctcctc gcccggccag aaacatccat tcctgcttca gctggaatat
     1741 cagattgatg tggtccagta ccatcagcac cagtgccacc gttttgatga tatcccgctg
     1801 tccgggtgac cagaccagaa atgactgcaa ccaggtatcg gtccgtgcag ccagtgaatc
     1861 gttacgtgtc gtgttcgtgt tatccgttgt catcagtgtt tgtcttcctg tatcagtctc
     1921 cgcccggggt tttctctttc tgcaggtcac gaaccatatc ccgctccatc tgctgcagac
     1981 gctcctgcag cagattttcc cgggcaacct cccggaccgc ttcccgctcc cgttgtgatt
     2041 cacgcagcac actctccggc agcgcggctt cccgttcagt tatggcagcc cggtcacgct
     2101 cctgcccggc aatttccctg acagccagct ctgttttgcc gtccggcagg tcaggtttgt
     2161 tctctgccat tttacggaca atttcatctg cgcgacgttc cgtttcacgg cggatggcct
     2221 cttcagcctg ccgctgtgcc agtacctctg ccgtgaccgg ttcgccattt ccggctcccg
     2281 gctggacgct gttgtccggg atatcccccc acacgcggcc atcggtgatg gcaccgggat
     2341 tccacggacg gccttcaccg gcaat
//
LOCUS       DAKISA010000255         2281 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.259, whole genome shotgun sequence.
ACCESSION   DAKISA010000255 DAKISA010000000
VERSION     DAKISA010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.259"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(25..315)
                     /locus_tag="OVW45_004503"
     CDS             complement(25..315)
                     /locus_tag="OVW45_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCV5528765.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(305..742)
                     /gene="ratA"
                     /locus_tag="OVW45_004504"
     CDS             complement(305..742)
                     /gene="ratA"
                     /locus_tag="OVW45_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCV5528766.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            913..1395
                     /gene="smpB"
                     /locus_tag="OVW45_004505"
     CDS             913..1395
                     /gene="smpB"
                     /locus_tag="OVW45_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCV5528767.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            1610..1972
                     /gene="ssrA"
                     /locus_tag="OVW45_004506"
     tmRNA           1610..1972
                     /gene="ssrA"
                     /locus_tag="OVW45_004506"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          635 a          602 c          507 g          537 t
ORIGIN      
        1 caatgagcac cttttttctg tctgttattt attcgctgat ttttctgctc gttgcctgcg
       61 aagctctttc ggatcggcaa tgagagggcg ataaatctcc acccgatcgc catcatgcac
      121 actatcgctt agttttgccg gacggctgta aatgccgact ttgtttttag ttaaatcgat
      181 atcggtacgc aattccagca agccactggc gcgaatagct tcttcaaccg tcgcgccctc
      241 ctgcagcgtc acgcgctgca ggtactgctt ctcaggtagc gcataagcca cctcaacggc
      301 aattttacct agcactgtaa acctctttcg cacgaaccgt aaaagcctgg accatattag
      361 acgccagctc tttaaacacg cgaccaaagg cgagttcaat caacttattg gtaaactcaa
      421 agtcgagatg aaactcgata cgacacgcct cctggctcag cggcgtaaac ttccatccac
      481 caatcaattt cttgaacggc ccatccacca gattcataag aatactttgg ttactggtca
      541 actggttgcg ggtagtaaac gttttgctga tcccagcctt agagacatct accgccgcag
      601 tcatctgccc aggagtggac tccagaatcc gacttccggt acaacccggc aaaaactgag
      661 gataagactg aacgtcattc actaactgat acatttgctc cgcgctgtag ggtaccagtg
      721 cggtccggct aatctgaggc ataactattt ccatcaacaa aaatccaaca aataatatca
      781 tttatcctgc tgtaaaaaaa acgctatccc ggcgctgggt aacatcgggt tcatgctaag
      841 atagagcctt gtcccccgca ggattgatat ggggtgtttt cgatttcaga ttaccgatga
      901 ttcacgacgc ttatgacgaa gaaaaaagca cataaacctg gttcagcgac catcgcgctt
      961 aacaagcgcg cccgtcacga atactttatc gaagaagagt tcgaagcggg acttgccctg
     1021 caaggctggg aagttaaatc cctgcgcgca ggaaaagcca atatcagcga cagctacgtc
     1081 cttctgcgtg acggagaggc atttctgttt ggcgctaaca tcacgccaat ggccgtggcc
     1141 tccacgcatg tggtgtgcga tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa
     1201 ctggactcat tgtacggtcg cgtcaatcga gaaggctata ccgtagtggc gctctccctg
     1261 tactggaaaa atgcctggtg caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac
     1321 gataaacgtt cagatatcaa agaacgcgaa tggcaggtgg ataaagcacg tatcatgaaa
     1381 aacgcccacc gttaaacctg cactccaatt attgaccagt tcctcaccgc gcctccctct
     1441 ccggcggcgc gaatgaacat cttattggct atcacatccg acacaaatgt tgccatccca
     1501 ttgcttaatc gaataaaaat caggctacat gggtgctaaa tctttaacga taacgccatt
     1561 gaggctggtc atggcgctca taaatctggt atacttacct ttacacattg gggctgattc
     1621 tggattcgac gggatttgcg aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa
     1681 aagccgcaaa aaatagtcgc aaacgacgaa aactacgctt tagcagctta ataacctgct
     1741 tagagccctc tctccctagc ctccgctctt aggacgggga tcaagagagg tcaaacccaa
     1801 aagagatcgc gtggaagccc tgcctggggt tgaagcgtta aaacttaatc aggctagttt
     1861 gttagtggcg tgtccgtccg cagctggcaa gcgaatgtaa agactgacta agcatgtagt
     1921 accgaggatg taggaatttc ggacgcgggt tcaactcccg ccagctccac cacttttgac
     1981 aggactgcaa ccggacagag actataaaaa cagtcactta cggacactga ccagacagta
     2041 agcggaccag aaaaagacaa aaatatacac gcaaaatgca cgtgcatttc aaaaaagcct
     2101 ctgattatga agtcagaggc ttttctagtt gtaaataagg gtaacaattt aacacgatgc
     2161 agcttaccaa ttacgtaaca tcaaacgctc taattgttcc caaatcctcc agaccactca
     2221 gctcctcttt catttcacgc tgacggcgat aaatctcatc gttgcgatcg acctgcgcct
     2281 g
//
LOCUS       DAKISA010000256         2247 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.260, whole genome shotgun sequence.
ACCESSION   DAKISA010000256 DAKISA010000000
VERSION     DAKISA010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.260"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..1008)
                     /gene="shiA"
                     /locus_tag="OVW45_004507"
     CDS             complement(<1..1008)
                     /gene="shiA"
                     /locus_tag="OVW45_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HCV5528768.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRVIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MA"
     gene            1323..>2247
                     /locus_tag="OVW45_004508"
     CDS             1323..>2247
                     /locus_tag="OVW45_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HCV5528769.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEGMRKKNLKTMLFISQLKAKTNFQVVGITMNCYSPLCKNASRMDQ
                     KLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIAFNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARALVDCYNNVYRLSLPPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIY"
BASE COUNT          696 a          500 c          502 g          549 t
ORIGIN      
        1 cgccataaag aaaggaaatg cgctcaacgt tccgattaac gcacctgtga tataaaccct
       61 acggcgacca aaacgatcgg caagccaggc aaaacaggga attgtcaggc agcttaatcc
      121 acctaccagc aaaccaatat taaggaaaag ttcgcgcggt agccccatat tctgggttga
      181 ataattaagt gcaaaggcag taacgatgta catcgtcagc aattcgcaca gtcgtagcgc
      241 aataatcttc aggaaagcac cgggatgtcg taacagcgct tcgataaccg ggatgcgttt
      301 tttcgcggca gcttgataat gttgctgttg ttcaaattcc gcggactcct ccatgccatt
      361 gcgcacccac aatgctccca gtaccagtac gatgctaaac aggaaaggaa tgcgccagcc
      421 ccagcttaaa aactgttcgt cagtcgtcat catactgatc aatgaaacca gtccggttga
      481 aagcagtaaa cctacaccgt agccaacttg tacaccgcta ctgtaaaagg cttttttatt
      541 tttcggtgca ctttcaacgg aaagcaacgc cgcgcctccc cattcgccgc cgacggcaaa
      601 tccctgaatg acacgcagtg tcaccagcaa aataggtgcc caccacccaa tggtcgagaa
      661 tgaaggaaga ataccaatca aggctgtcgc gatgcccatc atccagacgg tcagcattaa
      721 catgcgctta cgtcccagtc ggtcgccaaa gtgaccgaaa atgacaccgc cgagcggacg
      781 gaaaaggaat ccgacgccaa aggtagcaaa tgcggcgagc gttcccatcg ccgggcttac
      841 ttgcgggaaa aactcgcgat taaacaccag tgcggcggtg atgccataga gtaaaaaatc
      901 ataccagtcg acgacggcac cagcgaagct gcctaacgca gctcgtcggg cgcgacttaa
      961 cgaaagcgtc ccttcatcgg gacgagtgga gatgagcgtg gagtccataa tgatcctgtt
     1021 tgtactgtct ttttataatt agtatggaaa aggtaactca cattgccgtc gatctataaa
     1081 cgaaccttat gagactacct cgacagagcg catctgcaaa tgattttatg gctgattagc
     1141 ggcaacatga aaaattcgat ggaagatgaa agcgcaggag ttgctggtgt tgtttaagga
     1201 ttaagcagag gacccacagg ttagagatat gaaacataaa agtccgcata tgcacaaaat
     1261 ggcaccctga tgagtctgat agactgcatt gcattataac cagtagggag gcgaggttag
     1321 gtatgttttt agcatcattg ttgagacgta ttgcatttag ttactacgat tataaagctt
     1381 ataatttcaa tattgaaaaa acagactttg ttgtcatcca tattcccgat cagattggcg
     1441 atgctatggc catctttcct gttattcggg cgcttgaatt gcataaaatt aagcatcttt
     1501 taattgtaac gtcgacaatt aatttagaag tctttaatgc gcttaaactt gaacagacta
     1561 aattaacatt agtcacaatg actatgcagg atcacgcaac attaaaagaa ataaaagatt
     1621 tagcaaagaa cataacacag caatacggta cgccggatct ttgtattgag gggatgcgta
     1681 aaaagaacct gaaaacgatg ttatttatca gtcagttgaa agcaaaaacg aattttcagg
     1741 ttgttggtat aaccatgaat tgctactccc ctttgtgcaa gaacgcgtcc cgtatggatc
     1801 agaaactccg ggctcccgta cctatgacat gggcatttat gatgcgtgag gcgggttttc
     1861 cagcagtcag gccaatatat gaattgccac taagtgagga tgtactcgat gaggtgcgcg
     1921 aggaaatgcg atcgttagga tcttacattg cgttcaattt agaaggtagc tcgcaggaac
     1981 gtacattttc attatcgatt gcagaaaatc taatagcaaa aattcaaagt gaaacagata
     2041 taccaatagt gatcgttcat ggacccaaag gtgaagataa agccagggca ttagtggatt
     2101 gttataataa tgtctaccgt ttatccttac caccctcgat taaacgttca gcagcaatca
     2161 taaaagatgc ttatatcgca ataactcctg acacctcaat attacatatg gcaagtgcct
     2221 ataatacccc tgttgttgca atttatg
//
LOCUS       DAKISA010000257         2160 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.261, whole genome shotgun sequence.
ACCESSION   DAKISA010000257 DAKISA010000000
VERSION     DAKISA010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.261"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..453
                     /locus_tag="OVW45_004509"
     CDS             <1..453
                     /locus_tag="OVW45_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HCV5528770.1"
                     /translation="IAGEGRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQ
                     RQAEEAIRRETERRADEIVRKMAENKPDLPDGKTELAVREIAGQERDRAAITEREAAL
                     PESVLREPQRVREAVREVARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            502..921
                     /locus_tag="OVW45_004510"
     CDS             502..921
                     /locus_tag="OVW45_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001470742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528771.1"
                     /translation="METYPDPDDIRKNTADILKALTVDNIPERHGFREELASLKNCIN
                     DDEYCYMTFYETGYAFLKALLRTRLRLKRTDPAHSLLPLISSSVEALRAQLKENEAYV
                     RLLIGMDAVSRWTGPLFCFAALMILILVGTVFAHVWF"
     gene            943..1677
                     /gene="traX"
                     /locus_tag="OVW45_004511"
     CDS             943..1677
                     /gene="traX"
                     /locus_tag="OVW45_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HCV5528772.1"
                     /translation="MENTNNTASVVMKTREWPERLLVWSPGQRDIIKTVALVLMVLDH
                     INLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHANIRQPAINRLWGWGIIAQFAYYL
                     AGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAGLLML
                     AVSHRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSCAGKS
                     LPRFWPGDFFPVFYACHLAVLGVLAL"
     gene            1732..>2160
                     /locus_tag="OVW45_004512"
     CDS             1732..>2160
                     /locus_tag="OVW45_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCV5528773.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACR"
BASE COUNT          481 a          541 c          693 g          445 t
ORIGIN      
        1 atcgccggtg aaggccgtcc gtggaatccc ggtgccatca ccggtggccg cgtgtggggg
       61 gatatcccgg acaacagcgt ccagccggga gccggaaatg gcgaaccggt cacggcagag
      121 gtactggcac agcggcaggc tgaagaggcc atccgccgtg aaacggaacg tcgcgcagat
      181 gaaattgtcc gtaaaatggc agagaacaaa cctgacctgc cggacggcaa aacagagctg
      241 gctgtcaggg aaattgccgg gcaggagcgt gaccgggctg ccataactga acgggaagcc
      301 gcgctgccgg agagtgtact gcgtgaacca caacgggtgc gggaagcggt ccgggaagtt
      361 gcccgggaaa atctgctgca ggagcgactg cagcagatgg agcgggatat ggtccgtgac
      421 ctgcagaaag agaaaacgct gggcggggac tgatgctgtt gacccggccg tttgtgttaa
      481 ccccataagg agattgctga tatggaaact taccctgacc ctgatgatat ccggaaaaac
      541 acagcagaca tactgaaagc gttaaccgtg gacaatattc ctgagcgtca tggcttcagg
      601 gaggagctgg catcgctgaa aaactgcata aacgatgatg agtattgcta catgacgttt
      661 tatgaaacgg ggtacgcttt cctgaaagcc ttactgagaa cccggctgag gcttaaaaga
      721 accgaccctg cgcattcgtt gctgcccctc atctcttcgt cggtggaagc actcagggct
      781 cagcttaagg aaaatgaagc ttatgtccgc cttttaatcg ggatggatgc ggtctcccga
      841 tggaccgggc ctctgttctg ctttgccgcg ctgatgatac tgattctggt gggaactgtg
      901 tttgcacacg tatggtttta aagctcagta aggcgtgatg atatggagaa tacgaataat
      961 acagccagtg tggtcatgaa aacccgggag tggccggagc gtctgctggt ctggtcaccc
     1021 ggacagcggg atatcatcaa aacggtggca ctggtgctga tggtactgga ccacatcaat
     1081 ctgatattcc agctgaagca ggaatggatg tttctggccg ggcgaggagc ctttccgctg
     1141 tttgccctgg tgtgggggct gaatctgtcc cgtcatgcga atatccggca accggccatt
     1201 aaccggctgt ggggatgggg gattattgcc cagttcgcat attatctggc aggctttccc
     1261 tggtatgagg ggaatatcct gtttgccttt gcagtggcgg cacaggtgct gacgtggtgt
     1321 gaaacgcgca gtgggtggcg tacagcagcg gccattcttc tgatggccct gtgggggcct
     1381 ttgtccggca ccagttacgg cattgccggg ctgctgatgc tggcagtcag ccaccggctg
     1441 taccgggcgg aagacagagc ggaacgtctg gcgctggtcg cctgcctgct ggctgttatt
     1501 ccggcgctta accttgccac cagtgatgca gcggcggtgg ccggtcttgt gatgacggtg
     1561 ctgaccgttg gtctggtgtc gtgtgcagga aaatcattac cccgtttctg gcccggggat
     1621 tttttcccgg tattctacgc ctgtcatctg gctgtgctgg gcgttctggc gctgtgacgg
     1681 gtgtggtatc tttggccgca agaggatgat tcgtcagagg cagaacacag catgacagag
     1741 cagaagcgac cggtactgac actgaagcgg aaaacggaag gggagacgcc tgtccgcagc
     1801 cggaaaacca tcatcaatgt caccacgcca ccaaagtgga aggtgaaaaa gcagaagctg
     1861 gccgagaaag cagcccggga agcggagttg gcggcaaaaa aagcgcaggc cagacaggcg
     1921 ctgtccattt atctgaacct gcccacactg gatgaggccg tgaacaccct gaagccctgg
     1981 tggccgggat tgtttgacgg tgacacaccc cggcttctgg cctgtggtat ccgggacgtg
     2041 ttactggaag acgtggcgca gcggaatatc ccactctcgc ataaaaaact gcgcagggcg
     2101 ctgaaggcca tcacccgttc agaaagctat ctgtgtgcca tgaaagccgg tgcctgccgg
//
LOCUS       DAKISA010000258         2120 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.262, whole genome shotgun sequence.
ACCESSION   DAKISA010000258 DAKISA010000000
VERSION     DAKISA010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.262"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(1..606)
                     /locus_tag="OVW45_004513"
                     /pseudo
     CDS             complement(1..606)
                     /locus_tag="OVW45_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170527.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(970..1632)
                     /locus_tag="OVW45_004514"
     CDS             complement(970..1632)
                     /locus_tag="OVW45_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCV5528774.1"
                     /translation="MSIEHILIGKHHGGSDYWRTPFLLFKNLHREFIFSLDGAATEHD
                     TLLPRFTDDISRQSWVSEKVFCNPPYSDIPSFLLKASEADLVAFLIPHRANTSYWLRH
                     IYSNNHCHEIRILHRAVKYLPPAGHNRLTIRSPFPSAVVVFKKEPRKHEITQMVCCAD
                     TLLPLTIINRGGLRGRPTIYPPETLDSFIKLYRQGKPIKYIADALRMPLSTSYRIAQR
                     LS"
     gene            complement(2001..>2120)
                     /locus_tag="OVW45_004515"
     CDS             complement(2001..>2120)
                     /locus_tag="OVW45_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCV5528775.1"
                     /translation="RPVKASSEAVQMLISQGKTRLQIQEELGISRATYYRLAK"
BASE COUNT          623 a          420 c          385 g          692 t
ORIGIN      
        1 tttagagctt acttcaaggt cagattcatt catagagaga aaatcattta attcatccaa
       61 agaaagaaag tcatcttctt taatatcatc attaagtcca gtctctttat tcttatctgt
      121 aatattatta tctaatgttt tatctaataa cttatctaac tctaacacgg gtgacatttt
      181 gtcactactc tatctaacac gggtgacatt ttgtcactac ccccatgaca ttttgtcact
      241 ggtagtggtg tcattttgtc ttactgaggc tgcaacttcc tgtgatgtag gtcttacaag
      301 taattccggc ttatcatcca gtgtgaatac acgataagtg ctttgacgcc cctgattctt
      361 ttgcacctga attaaaccgt gtttctcaag tgtctcaatg gctctgattg ctgttctctt
      421 tgatacacca gccatataag cgagatggtc aatacttggt gaatatgatt tgtatttctc
      481 ttcatagaag tcacagctat ggaccatgac catataaatc aatcttgctg ctgcgttaat
      541 ttcttctgaa agttgagaga ttcgcacatc aaatttataa aactgatttg ggttatattt
      601 agacattcat aaattacctc atacgtgaaa aggccaaccg gataaaaccg attagcctgt
      661 gtgattaaaa tatctggggg attgatggac tgataaagaa gtccatgcct gaaacagtac
      721 gatgattaaa acgctgaggt ataccctgaa tgagaggtta atatacgcca accatctgac
      781 acctccccaa gtgttaccac ttcaagacat gaactttttt atcagcccat atagaaacta
      841 taacagatgt gattataaat tgcaacaatt atttcacaga atgattactg attggtcatt
      901 tattgttcac ggggatactt cttaacatct gaatcacctg tcttgtaaat gaccatattt
      961 atgagaattt tagctcaacc tttgagcaat cctgtatgac gtgctcagag gcattctgag
     1021 agcatctgca atgtacttaa tgggttttcc ttgcctgtat agttttatga agctgtcgag
     1081 cgtctcaggt gggtaaatgg tgggtcttcc tcttagtcca ccacggttaa tgatggttaa
     1141 cggtagcaat gtatcagcac agcaaaccat ctgtgtaatt tcatgcttac gtggttcttt
     1201 tttaaaaaca acgactgcac ttggaaacgg tgacctaata gtaagcctgt tatgccctgc
     1261 tggtggaagg tatttaactg cacgatgaag aatacggatt tcatgacagt ggttattgct
     1321 gtaaatgtgc ctaagccagt agctggtgtt ggctctatgc ggtatcagga aagctactaa
     1381 atccgcttca gacgctttta agaggaacga cggaatatcg ctgtacggcg ggttacagaa
     1441 gaccttttca ctaacccatg attgacggct tatatcatcg gtgaatcgtg gtaacagcgt
     1501 gtcatgctca gttgcagcac catctagact gaaaataaat tcacggtgta agttcttaaa
     1561 taacaaaaat ggagttctcc agtaatcaga acccccatga tgcttcccga ttaaaatatg
     1621 ctcaattgac ataagaaata aaaattttta ttgcaatcac tcttgacaag gtttactctc
     1681 ttttaagaga tacttaacag gttttctaaa cggatatcct ttctgggata tctatctttt
     1741 atcttaaaag tttttacaga tactttaaag atatctgtat atattctgta cagtaacttt
     1801 tcagagtctg aaaattttta taaaaaatga ctagccctct ttgaatctca tcttccccga
     1861 atcttcgaaa atatctgtct agttgattcg gtagaatcta cctcatgatg tatacgtgtt
     1921 tagtacccgt cacaccagct atgtgacccc cttggcaaaa aagccaaata tcagctacat
     1981 ccatgtaact attcagtaac ttatttagca agtctgtaat aagttgctct agagattcct
     2041 agctcttcct gaatctgcaa cctcgtttta ccttgtgaaa taagcatctg tacagcctct
     2101 gaagaagctt ttacaggtct
//
LOCUS       DAKISA010000259         2101 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.263, whole genome shotgun sequence.
ACCESSION   DAKISA010000259 DAKISA010000000
VERSION     DAKISA010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.263"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(60..1025)
                     /locus_tag="OVW45_004516"
     CDS             complement(60..1025)
                     /locus_tag="OVW45_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HCV5528776.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1891..>2101)
                     /locus_tag="OVW45_004517"
     CDS             complement(1891..>2101)
                     /locus_tag="OVW45_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528777.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          582 a          551 c          439 g          529 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcagtc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag aattgcgcaa
     1321 aaaataacca caattttaac cccccaaaac aagcaccccc gaacggctta ccccaacctt
     1381 ttccacacac ccgatcacca aaacagggaa taaaaacaaa accctgtctt acaggtggat
     1441 ggcctcactt cgtgaaacca tccaccccgc ataagttttt taatcggtgg tgtcgtcaat
     1501 cactaaatcg gaaccccaaa ggggaccccc acgatttagc atttgacgag attaactctc
     1561 ggagatggca tcatgctttt aacggacttt aaaaaacggt gaacggatgc ggattttaat
     1621 ccgtgtctgt gaggcgttga gcaacgagca aagcgaggcg gtagaaagag agccagcggc
     1681 aggctcagga cgataatccg gggaaaatat accactcgca gagtgttaac atttgacatc
     1741 ggattgtcta tcctgcattg gtgtcgagtt ggcaaggctg aggataatgc tgaggataat
     1801 gctgaggata atgcaggata gacagagcac tagagattgc atgcaatctc gtacctatca
     1861 cctcagcctg ccgctggctc ttttccagaa ctaagaatgc gttcttaaaa atccaaactt
     1921 gataccgttt aaatctattt tcacaaaaag agtttttacc ctctcgtttg ttttctgaat
     1981 atataaaatt aattcatcct caaaaccttc gtgcggatga aaaccaaatg tgccaaaact
     2041 taatccatca ataagcaaaa taaggttttt aaacctgtca ggaatcaccg cttcacttct
     2101 t
//
LOCUS       DAKISA010000260         2044 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.266, whole genome shotgun sequence.
ACCESSION   DAKISA010000260 DAKISA010000000
VERSION     DAKISA010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.266"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..505
                     /locus_tag="OVW45_004518"
     CDS             <1..505
                     /locus_tag="OVW45_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001690050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCV5528778.1"
                     /translation="VRRFHQVFTDWPLSGEYSCVQLARALFPGRYPSAPTVTQTVKTE
                     KNRRNRFSPEFKWRLVQQTLLPGACVAQIARENGINDNLLFNWRHLWRNGGLQPTGEH
                     ETSLLPVTLTPEPDNKIPAPVQIPEQMPDSLCCELVLSAGTLRLKGELTPALLQTLIR
                     EMKGSSH"
     gene            505..852
                     /gene="tnpB"
                     /locus_tag="OVW45_004519"
     CDS             505..852
                     /gene="tnpB"
                     /locus_tag="OVW45_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCV5528779.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWPVTRDGKVHLTPAQLSMLLEGINW
                     KHPKRTERAGIRI"
     gene            872..>2044
                     /locus_tag="OVW45_004520"
     CDS             872..>2044
                     /locus_tag="OVW45_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCV5528780.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAKYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQERISALQEEMAETLGEQYDPVLPS
                     ALRQSSARKPLPTSLPRETRVIRPEEECCPACGGDLSPLGCDVSEQLELISSAFKVIE
                     TQRPKLACCRCDHIVQAPVPSKPIARSYAGAGLLAHIVTGKYADHLPLYRQSEIYRRQ
                     GVELSRATLGRWTGAVAELLEPLYDVLRQYVLMPGKVHADDIPVPVQEPGSGKTRTAR
                     LWVYVRDDRNAGSQMPPAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRALYESG
                     RITEAACMAHARRKIHDVHARVPTDITTEALQRIGELYVIEAEVRGCSAEQRLAARK"
BASE COUNT          481 a          571 c          577 g          415 t
ORIGIN      
        1 ggtgaggcga tttcaccagg tattcactga ctggcctctt tccggggaat attcctgcgt
       61 gcaactggcc cgggctttgt tcccggggcg atacccttca gccccgacag taactcagac
      121 tgtgaaaaca gagaaaaacc gccggaaccg attttcaccg gagtttaaat ggagacttgt
      181 tcagcaaact cttttacccg gtgcctgtgt cgcacaaata gctcgtgaga acggaatcaa
      241 cgataacctg ctctttaact ggcggcatct ctggcgtaac ggcggcctgc agccgaccgg
      301 cgaacatgaa acatcgctac ttcccgtgac gttaactccg gagccggata ataaaatccc
      361 ggcaccagtg cagatacctg aacagatgcc cgacagtctg tgctgtgagc tggttctgtc
      421 ggccggaact ctcaggctga aaggtgaact gacaccggcg ttattacaga cacttatccg
      481 cgaaatgaaa gggagcagcc actgatgata tctctccctg ccggttcgcg tatctggctg
      541 gttgccggta tcaccgacat gcgaaatggt tttaacggcc tggcatcaaa agttcagaac
      601 gtcctgaagg atgacccgtt ctccgggcat ctgttcatct tccgcggacg ccggggtgac
      661 cagataaaag tgttgtgggc tgacagtgac ggactgtgcc tcttcaccaa acgcctggag
      721 cggggccgct tcgtctggcc ggttacccgt gacggcaagg tgcaccttac tccggctcag
      781 ttatctatgc ttcttgaagg tatcaactgg aagcacccga aacgaacgga acgcgctggc
      841 atccgcatat aacccgttgt aaagtgagga tatggacacc tcacttgctc atgagaatgc
      901 ccgcctgcgg gcactgttgc agacgcaaca ggacaccatc cgccagatgg cgaaatacaa
      961 ccgcctgctc tcacagcggg tggcggctta tgcttccgaa atcaaccggc tgaaggcgct
     1021 ggttgcgaaa ctgcagcgca tgcagttcgg taaaagctca gaaaaacttc gcgctaaaac
     1081 cgaacgacag atacaggaag cacaggagcg aatcagcgca cttcaggaag aaatggcgga
     1141 aacgctgggt gagcaatatg acccggtact gccatccgcc ctgcgccagt cttcagcccg
     1201 taaaccgtta ccgacctcac ttccccgtga aacccgggtc atccggccgg aagaggaatg
     1261 ctgtccggcc tgtggtggtg acctcagtcc tctggggtgt gatgtgtcag agcaactgga
     1321 gcttatcagc agcgctttta aggttatcga aacacaacgt ccgaaactgg cctgttgccg
     1381 gtgcgaccat atcgtgcagg caccagtacc ttcaaaaccc attgcacgca gttatgccgg
     1441 agcggggctt ctggctcata tcgtcaccgg gaagtatgca gaccatctgc cgttataccg
     1501 ccagtcagaa atataccgtc gtcagggcgt ggaactgagc cgcgccacgc tggggcgctg
     1561 gaccggtgcc gttgctgaac tgctggagcc gctgtatgac gtgctgcgcc agtatgtact
     1621 gatgcccggt aaagtccatg cggatgatat ccccgttccg gtccaggagc cgggcagtgg
     1681 taaaacaaga actgcccggc tgtgggtcta tgtccgtgat gaccgtaacg ccggttcaca
     1741 gatgcccccg gcggtctggt tcgcgtacag cccggaccgg aaaggtatcc atccacagaa
     1801 tcacctggcc ggttacagcg gtgtgcttca ggcagatgct tacggtggtt accgggcgtt
     1861 atacgaatcc ggcagaataa cggaagccgc gtgtatggcc catgcccgga gaaaaatcca
     1921 cgatgtgcat gcaagagtgc caaccgacat caccacggaa gccctgcagc gtatcggtga
     1981 actgtatgtc atcgaggcag aagtccgggg atgttcagca gaacagcgtc tggcagcaag
     2041 aaaa
//
LOCUS       DAKISA010000261         2023 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.267, whole genome shotgun sequence.
ACCESSION   DAKISA010000261 DAKISA010000000
VERSION     DAKISA010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.267"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..353)
                     /locus_tag="OVW45_004521"
     CDS             complement(<1..353)
                     /locus_tag="OVW45_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCV5528781.1"
                     /translation="MLNQETAKAARTDSGYILRAPRRMRVADAVAQYMRVPMGAGNSV
                     PWDPLVAPYVIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWVIYNVICDPADMLII
                     QMTEEKAREHSKKRLA"
     gene            complement(353..847)
                     /locus_tag="OVW45_004522"
     CDS             complement(353..847)
                     /locus_tag="OVW45_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HCV5528782.1"
                     /translation="MDRELKNLTLNISQLAALSGVHRQTAAARLQNLPVAGGHESNLK
                     LYRVVDIVSAFLALPPPVAEGEMDAHERKAWYQSERERLKFEQETAQLIPASDVRREF
                     AIWAKAVVQVLETLPDILERDCGLQPAAVSRVQSIIDDLRDQIALRVTEAGADDEEEL
                     QQEE"
     gene            1205..1381
                     /locus_tag="OVW45_004523"
     CDS             1205..1381
                     /locus_tag="OVW45_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528783.1"
                     /translation="MRDKPANHKRGKNPVCIVFDYSRTLAQKEFPVGLRSWLIREYGD
                     DTAHDVSGLNTFIR"
     gene            complement(1523..1675)
                     /locus_tag="OVW45_004524"
     CDS             complement(1523..1675)
                     /locus_tag="OVW45_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528784.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGAHFWSESRLNISLLMLDIEHFA
                     RGKGLR"
     gene            complement(1663..>2023)
                     /locus_tag="OVW45_004525"
     CDS             complement(1663..>2023)
                     /locus_tag="OVW45_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCV5528785.1"
                     /translation="RDKNARELKLANATITDMQQRQRDADALDAKYTKELADAKAEND
                     ALRRKLDNGGRVLVKGKCPVPSSAETSSASGMGNDATVELSPVAGRNVLGIRDGIIRD
                     QTALRTLQEYIRTQCLR"
BASE COUNT          498 a          557 c          477 g          491 t
ORIGIN      
        1 gcgagtcgtt ttttggagtg ttcgcgggct ttttcctccg tcatctgaat aatcagcata
       61 tcagcaggat cgcaaatcac gttgtaaatc acccagccgt caatcaggcc gatagtcttg
      121 ccggttcgtg ccgggccaac aaatatcact gcgtcgtatt cacgcgaggc caggcagttc
      181 atcggctcaa taacatacgg tgccaccagc ggatcccacg ggactgagtt ccctgccccc
      241 atgggcaccc gcatatactg agcaacggca tcagcaaccc gcattcgtct cggtgcgcga
      301 aggatataac ctgaatcggt tcgtgctgcc tttgcggttt cctgattcag cattactcct
      361 cctgctgtaa ttcctcctca tcatccgcac ctgcttcagt cacccgcagg gctatctgat
      421 cgcgcagatc atcaataatg gactgaacac ggctcacagc ggcaggctgc agaccgcagt
      481 cacgttcaag aatatccggt aatgtctcca gcacctgcac gaccgctttt gcccagatgg
      541 caaactcccg tctgacatca ctggccggaa tgagttgtgc cgtttcctgt tcgaacttaa
      601 gacgctcacg ttcagactga taccaggctt tgcgctcatg cgcgtccatt tcgccttctg
      661 caaccggcgg tggtaatgcc agaaatgccg acacaatatc aaccacccga taaagcttga
      721 ggttgctttc atgcccccct gcaacgggta gattttgcag ccttgccgca gcagtctggc
      781 gatgtacacc tgacagtgcc gccagttgac tgatattcag cgtcagattt tttaactctc
      841 gatccatacc cgctccagaa tgttttaaac atgcatcttg cgaacaactt taggcaaacg
      901 gtgttagtga tgaacaaaaa acaatcaaaa tcgacaccat aaaaataaaa ccactgtaat
      961 atcaatccat tacagtagtg gtgatgacga atgaaatttc aaaaactagc ctttttccgc
     1021 gccgctcccg ccccgtggca ggccacccca ccgggaggac ccgtcagcct gacagccatg
     1081 acgaacgtct gatacagcgc cctgcatgaa tgacatcggg ataatccaga aaggaatagc
     1141 atcgtaccca caagaatcag tgtaagtgtc ctgtttcttc cacccccgca caggactggc
     1201 gagcatgagg gacaaacccg cgaaccataa acgcggtaaa aacccggtgt gcatcgtttt
     1261 tgattattcc cgcacactcg cgcagaagga gttccccgtc gggctacggt catggttaat
     1321 acgggaatac ggcgacgata cagcgcatga tgtgtcaggc ttgaatacct ttatccgtta
     1381 aaagggatat cagttaagtt atcccgtgta gggtataagc cattatcaaa gccactctgt
     1441 aggaagtggc ttttgtaatg gcaataaaaa gccccgcgaa tgcgaggcta aatcctggta
     1501 tttgtaatga ctggctctta tctcaacgca gccccttacc gcgcgcaaaa tgctcaatat
     1561 caagcatcag caatgagatg tttaatctgg attcactcca gaagtgagca ccaccctgtc
     1621 tacagagcca gatgtgaagg atgatgagta aaattatcgc tatcatcgaa ggcattgcgt
     1681 cctgatgtat tcctgaagcg ttctcagtgc tgtttggtcg cggataattc cgtcccggat
     1741 accgagaacg tttcgtccag caactggaga gagttcgacg gtggcatcat tgcccatgcc
     1801 ggaggcgctg gaggtttcgg ctgaggatgg cacagggcat tttcctttga cgagcacccg
     1861 accaccatta tcaagcttgc gccgaagagc atcattttca gctttcgcat cagctaactc
     1921 cttcgtgtat ttagcatcga gtgcatcagc atcacgctgg cgctgctgca tgtcagtaat
     1981 ggttgcgttc gccagcttca gttctcttgc gtttttgtcg cgc
//
LOCUS       DAKISA010000262         2013 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.268, whole genome shotgun sequence.
ACCESSION   DAKISA010000262 DAKISA010000000
VERSION     DAKISA010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.268"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..389
                     /locus_tag="OVW45_004526"
     CDS             <1..389
                     /locus_tag="OVW45_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528786.1"
                     /translation="FDLSLEIPLPPPGILSKTVVPGESSATVKQRVMAARERQFKRQN
                     KLNAWLDSPEIRQFCKLESEDAMWLEGTLIHLGLSIRAWQRLLKVARTIADIDQSDII
                     TRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(414..752)
                     /gene="maoP"
                     /locus_tag="OVW45_004527"
     CDS             complement(414..752)
                     /gene="maoP"
                     /locus_tag="OVW45_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCV5528787.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            871..1710
                     /gene="hdfR"
                     /locus_tag="OVW45_004528"
     CDS             871..1710
                     /gene="hdfR"
                     /locus_tag="OVW45_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCV5528788.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(1806..1881)
                     /locus_tag="OVW45_004529"
     tRNA            complement(1806..1881)
                     /locus_tag="OVW45_004529"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1846..1848),aa:Trp,seq:cca)
     gene            complement(1890..1966)
                     /locus_tag="OVW45_004530"
     tRNA            complement(1890..1966)
                     /locus_tag="OVW45_004530"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1930..1932),aa:Asp,seq:gtc)
BASE COUNT          531 a          486 c          488 g          508 t
ORIGIN      
        1 gcttcgatct ctcactggag atcccattac caccccccgg cattttgagt aaaacggtag
       61 tgccgggaga aagcagcgcc accgttaaac aacgcgtaat ggccgccaga gagcgccaat
      121 ttaagcggca gaataaactg aatgcctggc tggatagtcc ggaaatacgc caattctgca
      181 aacttgagag cgaagatgcg atgtggttgg aaggaacact gatccatctg gggttatcga
      241 ttcgtgcctg gcagcggtta ttgaaagttg cacgaaccat tgctgatatt gatcagtctg
      301 acattatcac acgtcagcat ttgcaggagg cagttagcta tcgtgcgatt gaccgtttgc
      361 tcatccatct gcaaaaacta ctgacataaa aaaagggcat ttcgcccttt ttattaatca
      421 tcagaatcgg tgtagtcttc agcaccttca acctgcggtt taccgccgga aagggtgtga
      481 aaacgttttg gacgcttaat acgcgtcata tacttggacc acacgcgttc tgcttctgtc
      541 actggctcac gttcgccacg gcatactgct acgaagagtt tctcttcctc ggtaaccggc
      601 tcgcgtttgc caagatccaa ctcattgaag gcataaccat gacgctcaag cagttgtgcc
      661 tctttgatgg tgaaatcacc atgacgagag aatccacgtg gataatgttt attgtcgaaa
      721 tatcgattag tcgtcgtaaa gctttccgcc atcctgcacg ctcctaattc tttgaccgag
      781 ctagttatgg cgcggagtat tagttacgct tgacagagtg taaaacaaaa catttaaatc
      841 ataacgacaa ataattttgc ggagagcact gtggatacgg aattgttaaa aactttcctg
      901 gaagttagcc gaacgcgtca ctttggtcga gcggctgaat cgctctatct gacccagtca
      961 gcagtgagct ttcgaatcag gcaactggaa aatcaactgg gtgtgaacct tttcacccgc
     1021 cacagaaaca atatccgttt aaccgctgcc ggtgaaaaac tactgcctta tgcagaaacg
     1081 ctcatgagca cctggcaggc cgcccgcaag gaggtggcgc atacctcacg acataacgag
     1141 ttttctatcg gtgccagcgc ctcgttgtgg gaatgtatgc ttaatcagtg gttgggacgc
     1201 ttgtatcaaa atcaggatgc ccatacaggc ttacagttcg aagcgcgaat tgcccaacgg
     1261 cagtctctgg taaaacagct gcatgaacgc cagcttgatc ttcttattac cactgaagcg
     1321 cccaaaatgg acgaatttag tagtcagttg ctggggtatt tcactttagc gctttatacc
     1381 agtgcccctt ctaaactaaa gggagatctt aactatctgc gactggagtg ggggccagat
     1441 tttcaacagc atgaggcagg tttgatcggt gctgacgaag ttcccattct gacaaccagt
     1501 tctgctgaac tggcacagca acagattgcg atgcttaatg gttgcacctg gctacccgtt
     1561 agctgggcgc gtaaaaaagg cggcctgcat accgttgtcg atagcacaac actttcacgg
     1621 ccgctttatg ccatatggct gcaaaatagc gataaaaatg cgttgattcg cgatcttttg
     1681 aaaattaacg tgctggatga agtgtattaa tctgaatggc tggcaaggat gccggtagaa
     1741 ggatttactt cggagaggat tatttcagat aaaaaaaatc cttagctttc gctaaggatg
     1801 atttctggca ggggcggaga gactcgaact cccaacaccc ggttttggag accggtgctc
     1861 taccaattga actacgcccc taattagggt ggcggaacgg acgggactcg aacccgcgac
     1921 cccctgcgtg acaggcaggt attctaaccg actgaactac cgctccaccg aattctttta
     1981 caaccaccgg ttttatgacc ggcttactgc tta
//
LOCUS       DAKISA010000263         1985 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.269, whole genome shotgun sequence.
ACCESSION   DAKISA010000263 DAKISA010000000
VERSION     DAKISA010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.269"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..195
                     /locus_tag="OVW45_004531"
     CDS             <1..195
                     /locus_tag="OVW45_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528789.1"
                     /translation="SPKKIGFYTIGDKSIYLYDLRRMDEIMEALDNRSSMDWCVAVHD
                     MNAGFDEKILFPSSVESTAG"
     gene            206..373
                     /locus_tag="OVW45_004532"
     CDS             206..373
                     /locus_tag="OVW45_004532"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528790.1"
                     /translation="MTTITKERIELFIKNPLENGLTRGEQMELARIALASLEAEPVVF
                     WFEKYQEGATA"
     gene            481..714
                     /locus_tag="OVW45_004533"
     CDS             481..714
                     /locus_tag="OVW45_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528791.1"
                     /translation="MDIAVLEIALVSLAAEPAGKLHEYKPVGYQRLVDELTMLVKQLT
                     WQLRKAKPDCKLPDKAMSYLERNGLISVEDILR"
     gene            1006..1161
                     /gene="hokD"
                     /locus_tag="OVW45_004534"
     CDS             1006..1161
                     /gene="hokD"
                     /locus_tag="OVW45_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCV5528792.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1382..1642
                     /locus_tag="OVW45_004535"
     CDS             1382..1642
                     /locus_tag="OVW45_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528793.1"
                     /translation="MKIEELREIFSEDGLYTVRVENGAIVSHCRIKCLQSQQRKSGAA
                     LIHFVDGLVTDGFISRANEFVTSLPSLKEAGIKAGFSAFEDE"
     gene            1709..>1985
                     /locus_tag="OVW45_004536"
     CDS             1709..>1985
                     /locus_tag="OVW45_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528794.1"
                     /translation="MAQNSRLHNSDNSAVFASRHGRRSHAFKSDWYQHPPCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSERPTRPSRTFQQRIWR"
BASE COUNT          550 a          425 c          532 g          478 t
ORIGIN      
        1 tcaccgaaaa aaattggctt ttacaccatt ggcgataaaa gcatttacct gtatgaccta
       61 cgccgcatgg atgaaatcat ggaggctctt gataatcgtt cgtcgatgga ttggtgtgtt
      121 gctgttcatg atatgaatgc agggtttgat gaaaagattt tgttcccctc atcagttgaa
      181 agcactgcgg gttaaggagt aacacatgac cactattacc aaagaacgta ttgaattgtt
      241 cattaaaaac ccgcttgaaa acgggcttac ccgtggtgaa caaatggaac tggcacggat
      301 tgcgctggca tcgctggaag cagagccggt tgtgttctgg tttgaaaaat atcaagaagg
      361 ggctacggca tgacgacttt taccagagag cagttaatag ctcacgcaga ggagactatt
      421 gaagcacaga gactgtgcat accgggcaca atcgaccatg acatcatccg cacatataag
      481 atggatattg ctgttctgga aatcgcactg gtatcgctgg cagcagagcc agccggtaaa
      541 ttgcatgaat acaaaccagt gggatatcag cgtctggtcg atgagttaac catgctggta
      601 aagcagttaa cctggcaact gaggaaagcg aagccagact gcaaattacc ggataaggcg
      661 atgagttatc tggagcggaa cggactgata agcgtggagg atattttacg atgacctggc
      721 ctgaagcatt aacaacggta ggaattgcga tggcggtggc gctggtggtg tattcgattt
      781 gccgctgggg ataaaaacgg tttgcgggaa aaggagagtt aagtagaatt gcagcgggtg
      841 cttgaggcta tctgtctcag gcatgaacac caaaaggcag atagagaaaa gccccagtta
      901 acattacgcg tccggcaaga cgcttaacat taatctgagg ctcaatctat gaacggcaaa
      961 tctaggttag cctcttacgt gccgaaaggc aaggagaagc aggctatgaa gcagcaaaag
     1021 gcgatgttaa tcgccctgat cgtcatctgt ttaaccgtca tagtgacggc actggtaacg
     1081 aggaaagacc tctgcgaggt acgaattcga accggccaga cggaggtcgc tgtcttcaca
     1141 gcttacgaac ctgaggagta agagaccagg cgggggagaa atccctcgcc acctctgatg
     1201 ggtcaggcat cctcaatgca cccacactta acccgcttcg gcgggttttt gtttttattt
     1261 tcaacgcgtt tgaagtttta gatggtgccg gaatagaatc aaaaatactt aagtagcgcg
     1321 cagggagaag agggatggac cccgaacagg ggagtgctat ttatctggaa ggattctgtt
     1381 gatgaaaatc gaagaattac gtgaaatttt tagtgaagat ggcctctata ctgtgcgcgt
     1441 tgagaatggc gctattgtca gccactgccg tattaaatgt ttacagtctc aacaaaggaa
     1501 gagtggagct gcgttaattc attttgtgga tgggcttgtg acggatggtt ttatttcgcg
     1561 tgcaaatgaa tttgtcacat cgttgccgtc tctgaaagaa gctgggatta aggctggttt
     1621 ttctgctttt gaagatgagt gaattcatct acaattcagc gcagggctga acccctgttg
     1681 agtaacactg tgccaccgga gaaagccgat ggcgcaaaat tccagactac acaattctga
     1741 taattcagcc gtctttgcca gcaggcacgg gcggcgctct cacgcattta aatctgattg
     1801 gtaccagcat cctccatgca ctgaagaaca ggccgaatgg ctcattcagt gttaccgcag
     1861 gcgcggatgc gaggttaaaa aagcccttag cctcgactac cgtcactgga taatctccgt
     1921 caggctccct tactccgaac ggccaacgcg tccgtcccgc acattccagc aacggatctg
     1981 gaggt
//
LOCUS       DAKISA010000264         1985 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.270, whole genome shotgun sequence.
ACCESSION   DAKISA010000264 DAKISA010000000
VERSION     DAKISA010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.270"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..265)
                     /locus_tag="OVW45_004537"
     CDS             complement(<1..265)
                     /locus_tag="OVW45_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCV5528795.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQEFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDT"
     gene            322..990
                     /locus_tag="OVW45_004538"
     CDS             322..990
                     /locus_tag="OVW45_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCV5528796.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            1306..>1985
                     /locus_tag="OVW45_004539"
     CDS             1306..>1985
                     /locus_tag="OVW45_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCV5528797.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
BASE COUNT          605 a          427 c          368 g          585 t
ORIGIN      
        1 ttgtgtctgg ttcttccggc cactcaatat caggtgcagt tgatgtatca acacggttca
       61 gcaacacccg atacttcttc caggcttcca gcaacgagtt ttcttcctcc gttgcgatct
      121 ccagatctac agcatcctga agtggcgcaa tatgctcact gaattcctgg atgtagaact
      181 gtgtggtgac ggtcttccag ccattcggct cctgctgtat cgaagcatac caggctattt
      241 caatatcgct atgctgcggc agcatttaac cccttgtaat tcatcgccat aattgattta
      301 attcacaaat aaaactataa tatggtgaaa ttaatgaaaa aaaacacaga tgatggggct
      361 aaaatttaca caccacttac cctaaagctt tatgactggt gggttttggg agtatcaaat
      421 cggcttgcat ggggatgtcc tacaaaggaa caccttcttc cacactttct ggaacattta
      481 ggtaacaacc atctggatat tggtgttgga actgggtttt accttactca cgtacctgag
      541 agtagtctga tatctttaat ggatttgaac gaagctagcc tgaacgcggc atctacaagg
      601 gctggggaat caaaaattaa acataaaatt agccatgatg tttttgaacc ttatcccgcg
      661 gcgttacatg gtcaatttga ttccatttcc atgttttacc ttcttcactg cctgcctgga
      721 aatatatcta caaaaagctg tgtaatacgc aatgcggcgc aggccttaac tgatgatgga
      781 actctatacg gagccacaat tcttggcgat ggagttgtgc acaatagctt cggtcaaaaa
      841 ctgatgcgca tttacaatca gaaaggcatc ttttcaaaca caaaagattc cgaagaaggc
      901 ttaacacata tactctcaga gcatttcgag aatgttaaaa ccaaggttca aggtactgta
      961 gtaatgtttt ccgcttcagg gaaaaaatag catccaaccg cagcacgttc ttgcttaaga
     1021 cgtgctacgg tttcctttac gatttggggt tgcgacttta cctctattga taatgcattc
     1081 cggcagaacg ttcaaatatg aacgtacgat atttaactaa ccgaaaaaca aaatatagca
     1141 caggcgagac atttaccatc taagaaaata gtatcgtttt tttacagtta aatcaacatc
     1201 tcttccttaa aatgaaaaca ataaatttca atcctgaaac attttatttc agcttattat
     1261 ttcccagtat cacattttat cagaatatcc ttctgcgagg ttattatgct tcctgtaaat
     1321 aatccccccc tatccactgg aaacgtctct ttttacagaa ctacatcaat cgacaatgtt
     1381 cacaataatt atctctccga atgggttgaa tggactaaaa acagcatttc cggagaaaac
     1441 agggaaactg cttttacccg gctccaatta tgtctggaga acagtgaaac atcgttggac
     1501 ttatcttgtt taggtctcag atctctacca cgattgcctg acaatcttga tgaaattaat
     1561 gtaagcaata accaactatc aatgctcccc gagctaccaa gggcattgaa agagctgaat
     1621 gcaagcagta atcaattatc tgcacttcct gaattaccag tgtcgctgga atatataaat
     1681 gtgagtgata accatttgtt cgcacttcct gaattacctg cgtcactaga atatattaat
     1741 gtaagtgaca atcacctgtc tgtacttccg aggttaccaa tgtcattgga attacttgat
     1801 gcagccagaa atgctttgga agtaatacca gattttccag aaagagatga tcatattata
     1861 agaatattct ggcttaatca gaaccggatc acggcaattc cggaaagcat acttggcctc
     1921 agttctgata gcgttgtcaa tcttagagaa aatcaactat ctcccagaat aatgcaaact
     1981 ttgtt
//
LOCUS       DAKISA010000265         1836 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.271, whole genome shotgun sequence.
ACCESSION   DAKISA010000265 DAKISA010000000
VERSION     DAKISA010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.271"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..628)
                     /locus_tag="OVW45_004540"
     CDS             complement(<1..628)
                     /locus_tag="OVW45_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCV5528798.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPAHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQK"
     gene            636..1137
                     /gene="tnpA"
                     /locus_tag="OVW45_004541"
                     /pseudo
     CDS             636..1137
                     /gene="tnpA"
                     /locus_tag="OVW45_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(1155..1823)
                     /locus_tag="OVW45_004542"
                     /pseudo
     CDS             complement(1155..1823)
                     /locus_tag="OVW45_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
BASE COUNT          464 a          440 c          423 g          509 t
ORIGIN      
        1 tcttctggtt tttctgaaaa ctgtttacag gctcaaagac tgtgccatct gacagcgtgg
       61 cgagtttagc cacgccagca tccagcccga ccattgatgc tgaagggtga gccggagtgg
      121 atacttcact ttctgtctga atactgatgt accacgtacc gcaggactgg ctgacagtga
      181 catttttcac aacacccgtg acctgacggc tattccggta gcgcatccag cccagtttcg
      241 gcagaaaaat acggctgttt tcctgatcga gcttaacgcc ctgcgggtag cggaatgcat
      301 cattctgtcc ccgctttttg aatcggggaa aagcagcccg cttccggaag aagtttttgt
      361 aagcccgctc aaggtctttc agtgactgtt gcaatggctg tgagggagaa tctttaagcc
      421 attgcgtttc agtggcattt ttccactcaa ccagccagga agccattttc ccgtaaggga
      481 tgtatttatt accggcctca tgattctcat tctgacgtgc cagagcacga ttgaaaacga
      541 aacgacatgc gcctgcgaag cgcctcattt cacactcctg ttgaccacct ggtcttaact
      601 ggaatttaaa tgcctgtaat cgtttcacag gtcaaatgat actttggtct gtgaaaaaag
      661 aaaccgatat ccggcgtgga aggcattgtg ttttcctgat gcatgttcac ctggtctttg
      721 tcactagata ccgacgccag attttttgac gcgacagaaa aactacgcac ttacttttca
      781 aatgtatgtg cttattttga agctgaactg gttgaaatgg atggcgaacc agatcacgtc
      841 catttgttaa ttaactaacc tcccaaactg gcgatatcca gtctggtaaa cagcctcaaa
      901 ggcgtatcgg gtaggttact gcgacgagat cgaccagata ttgcagtcag gtattactac
      961 aaaggcgttt tgtggagtcc tggctatttt gccagtagct gcggaggtgc gccaatatcc
     1021 gtcatccgcc aatacattga acaacagcaa acacctggtc aggtggaaaa ccgcgcctta
     1081 tatccccgcc ctgaaggatg gggttttacg gcgcaccgga taaaaagcgc catctaacga
     1141 tggcgcgtct ggaactactt tttgttaacg tcaaacatgg tggcatcggt cgccatgtca
     1201 tcaataactt gtttaatatt ttcgaaagcc attggcgtac tttcgttatt caggtcttta
     1261 ccttcgccct gacgaacgac tttgataacc ggtttattag tcgctgcatc aatcagctca
     1321 ccttcaaaat agaggcgagt atccatggta cggtggcctg tagccatttg cgtccccgca
     1381 accactaatg caacaggaac cacttcatag aattgcagcc cttctttgct ggtatctaca
     1441 ccggtaatgg ccccacggaa aatcagacta cgcggcccag cggtggtaac cagtggttta
     1501 cgctgcgcta tcgcttcttt catttcggtg ttggtatagt tcaaaatttt atccagaact
     1561 ttctgcccta cctaggtaga aggttttggc accggataat aagtgattgg gttccagacg
     1621 atgctgtcat atttgctttg atcaaaactc gggtctaccc aacgtaaaac aggtttacct
     1681 gttgctgagg ttgtttcttt taaatcagag taattgttta aaaaaccaga atatttatct
     1741 ggctgggtga ttttagaggc acaaccagac agagccaaca agccgcataa agctgcaact
     1801 ttcgcaaatg atgtggtacg catgaatagt ttcctg
//
LOCUS       DAKISA010000266         1811 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.272, whole genome shotgun sequence.
ACCESSION   DAKISA010000266 DAKISA010000000
VERSION     DAKISA010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.272"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            33..1811
                     /gene="fimD"
                     /locus_tag="OVW45_004543"
                     /pseudo
     CDS             33..1811
                     /gene="fimD"
                     /locus_tag="OVW45_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
BASE COUNT          477 a          407 c          443 g          484 t
ORIGIN      
        1 tgactgccgg gtgattttaa accggaggaa taatgtcata tctgaattta agactttacc
       61 agcgaaacac acaatgcttg catattcgta agcatcgttt ggctggtctt tttgtccggc
      121 tctttgtcgc ctgtgctttt gccgcacagg cacctttgtc atctgccgaa ctctatttta
      181 atccgcgctt tttagcggat gatccctagg ctgtggccga tttatcgcgt tttgaaaatg
      241 ggcaagaatt accgccaggg acgtatcgcg tcgatatcta tttgaataat ggttatatgg
      301 caacgcgtga tgtcacattt aacacgggcg acagtgaaca agggattgtt ccctgcctga
      361 cacgcgcgca gcttgccagt atggggctga atacggcttc tgtctccggt atgaatctgc
      421 tggtggatga tgcctgcgtg ccattaacct caatgatcca tgacgctact gcgcatttag
      481 atgttggtca gcagcgactc aacctgacga tccctcaggc atttatgagt aatcgcgcgc
      541 gtggatatat tcctcctgag ttgtgggatc ccggtattaa tgccggcttg ctcaattata
      601 atttcagcgg aaatagtgta cagaatcgga ttggtagtaa cagccattat gcatatttaa
      661 acctacagag tgggctaaat attggtgcgt ggcgtttacg cgacaatacc acctggagtt
      721 ataacagtag cgacagttca tcaggtagca aaaataaatg gcagcatatc aatacctggc
      781 ttgagcgaga cataattccg ttacgttccc ggctgacgct gggtgatggt tatacccagg
      841 gcgatatttt cgatggtatt aactttcgcg gcgcacaatt ggcctcagat gacaatatgt
      901 tacccgatag ccaaagagga tttgccccgg tgatccacgg tattgctcgt ggtactgcac
      961 aggtcactat taaacaaaat gggtatgaca tttataatag tacggtgccg ccggggcctt
     1021 ttaccatcaa cgatatctat gccgcaggta atagtggtga cttgcaggta acgattaaag
     1081 aggctgacgg cagcacgcag atctttaccg taccctattc gtcagtcccg cttttgcaac
     1141 gtgaagggca tactcgttat tccattacgg caggagaata ccgtagtgga aatgcgcagc
     1201 aggaaaaaac ccgctttttc cagagtacat tactccacgg ccttccggct ggctggacaa
     1261 tatatggtgg aacgcaactg gcggatcgtt atcgtgcgtt taatttcggt atcgggaaaa
     1321 acatgggggc actgggcgct ctgtctgtgg atatgacgca ggctaattcc acacttcccg
     1381 atgacagtca gcatgacgga caatcggtgc gttttctcta taacaaatcg ctcaatgagt
     1441 caggcacgaa tattcagtta gtgggttacc gttattcgac cagtggatat tttaatttcg
     1501 ctgatacaac atacagtcga atgaatggct acaacatcga aacacaggat ggtgttattc
     1561 aggttaagcc gaaattcacc gactattaca atctcgctta taacaaacgc gggaaattac
     1621 aactcaccgt tactcagcaa ctcgggcgca catcaacact gtatttgagt ggtagccatc
     1681 aaacttattg gggaacgagt aatgtcgatg agcaattcca ggctggttta aatactgcat
     1741 tcgaagatat caactggacg ctcagctata gcctgacgaa aaacgcctgg caaaaaggac
     1801 gggatcagat g
//
LOCUS       DAKISA010000267         1775 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.273, whole genome shotgun sequence.
ACCESSION   DAKISA010000267 DAKISA010000000
VERSION     DAKISA010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.273"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..401
                     /locus_tag="OVW45_004544"
     CDS             <1..401
                     /locus_tag="OVW45_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCV5528799.1"
                     /translation="PPKTDKKNVIQVTMQHALKLFWRPKAIELEDDGVMSYEKVEIIR
                     RNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVGY
                     INDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            403..1467
                     /locus_tag="OVW45_004545"
     CDS             403..1467
                     /locus_tag="OVW45_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCV5528800.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITYN"
     gene            complement(1487..>1775)
                     /locus_tag="OVW45_004546"
     CDS             complement(1487..>1775)
                     /locus_tag="OVW45_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCV5528801.1"
                     /translation="TYRRAKTITLIVDNYIIHKSRETQSWLKENPKFRVIYQPVYSPW
                     VNHVERLWQALHDTITRNHQCSSMWQLLKKVRHFMETVSPFPGGKHGLAKV"
BASE COUNT          529 a          347 c          374 g          525 t
ORIGIN      
        1 taccgccaaa aacagataaa aaaaatgtga tacaagtcac tatgcagcac gcattgaaac
       61 tattctggcg accaaaagct attgaattag aagatgatgg ggtaatgtct tacgaaaaag
      121 ttgaaatcat ccgtagaaat gatggttcga tccgctttaa caataagatg ccttatcacg
      181 ttacgctggg ttatatcgga acaaacggaa tcacaatgtt gccacaaacg caaagtttaa
      241 tggtaacccc ttatagccat gcagatacgc agtttaaaaa tgttccgtca gcttttcagg
      301 ttggttatat caatgatttt ggtggattaa gtttttatga aataaactgc ccgacggtta
      361 ataactcttg taatgtttcc gtagccaaac gagataaata atatgcgcta cttattaatt
      421 gtaattatat ttattatagg ttttagtgca ttacctgtat gggcaatgga ctgctatgct
      481 gaacatgaag ggggaaatac cgtagtcatt ggttacgtac caagaatttc tatccccagt
      541 aatggtaaaa aaggtgataa gatctggcaa agcagtgagt attttatgaa tgtcttctgt
      601 aataatgcat tacctgcccc atcacctgga gaagaatacc catcagcctg gactaatata
      661 atgatgtttt taccaggcgg tcaggacttt tataatcaga actcctatat ttttggcgta
      721 acatacaatg gagttgatta tgattcgact gcccctaatg ctatcgcagc accagaatgc
      781 attgatataa aaggagcggg tacctttaat aatcactata aaaaccccgc tgtctgtagt
      841 ggaggtcctg agcctcaatt atcagtaacc tttccagctc gcgtacaact ctatattaaa
      901 ttagcaaaaa atgccaatag agttaataaa aacctggtgt tacctgatga atatatcgct
      961 ctagagttta aaggtatgag tggcgcagga gctatagaag tagacaaaaa tttgaccttc
     1021 aggattcgtg gattaaataa tattcatgtc cttgactgct ttgttaatgt cgcactggaa
     1081 ccagctgatg gcgttgttga ctttggtaaa ataaattccc gaacaattaa aaataccagc
     1141 gttagtgaga cgtttagtgt agtcatgaca aaagatcctg gtgcggcctg tactgagcaa
     1201 ttcaatattt tagggagctt tttcactacg gatattttga gtgattatag ccatctggat
     1261 atgggtaatg gactgctatt gaagatattt cataaagatg gaacagcaac ggaatttaac
     1321 cgcttctcac aatttgcttc tttttcatcg tctagtgcac cttcggtcac cgcaccattc
     1381 agggcagaac ttagtgcgaa cccggcagag actgttgttg agggaccgtt tagtaaagac
     1441 gtaatcctca aaatcaccta taactaaata gctgcgctta ataccgctac acttttgcca
     1501 gcccatgttt gcctccgggg aatgggctga cggtttccat aaaatggcga acttttttca
     1561 acagttgcca cattgagctg cactgatgat tacgcgttat tgtgtcgtga agtgcctgcc
     1621 atagccgttc aacatgattc acccatggcg agtaaaccgg ctgataaatg accctgaact
     1681 tcgggttctc cttcagccag ctctgtgttt cccggctttt gtggataatg tagttgtcca
     1741 cgatcagcgt gatggttttc gcccgacggt atgtc
//
LOCUS       DAKISA010000268         1758 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.274, whole genome shotgun sequence.
ACCESSION   DAKISA010000268 DAKISA010000000
VERSION     DAKISA010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.274"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            15..1544
                     /gene="bglH"
                     /locus_tag="OVW45_004547"
     CDS             15..1544
                     /gene="bglH"
                     /locus_tag="OVW45_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005099837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HCV5528802.1"
                     /translation="MEQRLELLEKALRETQSELKKYKDEEKKKYTPATVNRSVSTNDQ
                     GYAANPFPTSSAAKPDAVLVKNEEKNASETGSIYSSMTLKDFSKFVKDEIGFSYNGYY
                     RSGWGTASHGSPKSWAIGSLGRFGNEYSGWFDLQLKQRVYNENGKRVDAVVMMDGNVG
                     QQYSTGWFGDNAGGENFMQFSDMYVTTKGFLPFAPEADFWVGKHGAPKIEIQMLDWKT
                     QRTDAAAGVGLENWKVGPGKIDIALVREDVDDYDRSLQNKQQINTNTIDLRYKDIPLW
                     DKATLMVSGRYVTANESASEKDNQDNNGYYDWKDTWMFGTSLTQKFDKGGFNEFSFLV
                     ANNSIASNFGRYAGASPFTTFNGRYYGDHTGGTAVRLTSQGEAYIGDHFIVANAIVYS
                     FGNDIYSYETGAHSDFESIRAVVRPAYIWDQYNQTGVELGYFTQQNKDANSNKFNESG
                     YKTTLFHTFKVNTSMLTSRPEIRFYATYIKALENELDGFTFEDNKDDQFAVGAQAEIW
                     W"
     gene            1571..>1758
                     /locus_tag="OVW45_004548"
     CDS             1571..>1758
                     /locus_tag="OVW45_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528803.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVS"
BASE COUNT          502 a          372 c          429 g          455 t
ORIGIN      
        1 ctgaatcatt aacggtggaa caacgccttg agttattaga aaaagcgtta agagaaacgc
       61 aaagcgaact caaaaagtat aaagatgaag agaagaaaaa atatacgcca gcgacggtga
      121 atcgtagcgt aagtacgaat gatcaagggt atgccgccaa tccgttcccg accagtagtg
      181 ccgcaaaacc tgatgctgta ctggtcaaaa atgaagagaa aaatgccagt gagacaggtt
      241 cgatttattc ttccatgact ctgaaagatt tcagtaagtt tgtgaaagat gaaattggct
      301 ttagttacaa cggctattac cgttctggtt gggggaccgc ctctcatggt tcacctaaat
      361 catgggcgat tggttctctg ggccgctttg gtaacgaata ctccggctgg tttgatttgc
      421 agttaaaaca acgtgtctac aacgaaaacg gcaaacgggt tgatgccgtt gtgatgatgg
      481 atggtaacgt tggtcagcag tactctaccg gctggtttgg cgataacgcc ggtggcgaga
      541 actttatgca gttctccgat atgtacgtta ccaccaaagg tttcctgccc tttgcgccag
      601 aggctgattt ctgggtgggt aaacacggtg cgccgaaaat tgaaatccag atgctggact
      661 ggaaaacgca gcgtactgat gccgcagcgg gtgtaggtct ggaaaactgg aaagtcggtc
      721 cgggtaaaat tgatatcgcg ctggttcgcg aagatgtcga tgattacgat cgcagcctgc
      781 aaaacaaaca gcagattaat accaatacta ttgatttacg ctataaagat atcccgttat
      841 gggataaagc gaccttaatg gtgagtggtc gttatgtcac ggcaaacgaa agcgcatcgg
      901 aaaaagataa tcaggataat aacgggtatt atgactggaa agatacctgg atgttcggca
      961 catctttaac gcagaaattt gataaaggtg gcttcaacga attctccttc ctggttgcga
     1021 ataactctat cgccagtaac tttggccgtt atgctggcgc aagtccattt accaccttta
     1081 atggtcgtta ttatggtgat cacaccggcg gaacagcagt tcgtctgacc tcgcagggcg
     1141 aagcctatat cggcgatcat tttattgtgg ctaacgcgat tgtttactcc ttcggtaacg
     1201 atatttatag ctacgaaaca ggcgcacact ctgattttga atctattcgt gcggttgttc
     1261 gcccggccta tatttgggac caatataacc agacaggtgt tgaactgggc tatttcaccc
     1321 agcaaaacaa agatgcgaat agtaataaat ttaatgagtc tggttataaa accacgctct
     1381 tccatacctt taaagtcaat accagtatgt tgacctcgcg tccggaaatt cgtttctacg
     1441 ccacgtatat caaagctctg gaaaacgaac tggatggctt cacctttgaa gacaataaag
     1501 acgaccagtt tgctgtcggt gcgcaggctg aaatctggtg gtaatggaac ttaatgaaag
     1561 gaatattacg atgaatataa aaattgctgc tttaacgctg gctattgcca gcggtatttc
     1621 tgctcagtgg gccatagcag cggatatgcc agccagcccg gcacccacta ttccggttaa
     1681 acagtatgtg actcaggtca atgccgataa cagcgtgacc tttcgctact ttgcccctgg
     1741 ggcaaaaaac gtctcctt
//
LOCUS       DAKISA010000269         1660 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.275, whole genome shotgun sequence.
ACCESSION   DAKISA010000269 DAKISA010000000
VERSION     DAKISA010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.275"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="OVW45_004549"
     CDS             complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="OVW45_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HCV5528804.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITYIPENILSLDPTCTIILEDNPLSSRIRESLS"
     gene            1494..>1660
                     /locus_tag="OVW45_004550"
     CDS             1494..>1660
                     /locus_tag="OVW45_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HCV5528805.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVI"
BASE COUNT          482 a          279 c          387 g          512 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tatgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 tgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct
      661 gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg ggacaatgaa
      721 aagttattat ttatcggtaa catgatggac attcagttaa agccgatgga caaatggctt
      781 tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac
      841 ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg tttcagttaa
      901 ctgtgaatag cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact
      961 tcatatttga acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat
     1021 cgtaaaggaa accgtagcac gtcgtatgca agaacgtgcc acggctggct gatggatgtt
     1081 cgatagcgcg agtttgaatg aaaatcagcc ggagatgatt ttacataatt gctacggaat
     1141 tattcaatac aggaattgct tgcgtctgca tggattgacc tgaaatattc ccgaaaattt
     1201 ctctaaaaaa ctcgaaaaaa atggtaactg gttgaatgta ttaatatgca atggtacgtg
     1261 tcagggatta aaagatgaac gtaaatttat ttaacgcatt aattttaaag ggttttattg
     1321 tttgttgacg aaaacaggaa tcgtgttcgg tctcttttta tctgttaaaa gccagaagca
     1381 tttccttcgc tgactttata gtcaaccata acacacactc tactgtctga gtccaacgtt
     1441 ttttaacatt cttgttaaaa ttatgtgatc tttagcgcgg gaggaaaata ttgatgaaac
     1501 agcctgcgcc cgtttatcag agaattgcgg gtcatcaatg gcgacatatc tggctttctg
     1561 gcgatataca cggttgtctt gagcagttgc gccgcaaatt atggcattgt cgttttgatc
     1621 cgtggcgaga tttacttatc tcagtgggag acgttatcga
//
LOCUS       DAKISA010000270         1591 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.276, whole genome shotgun sequence.
ACCESSION   DAKISA010000270 DAKISA010000000
VERSION     DAKISA010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.276"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(688..1359)
                     /gene="queE"
                     /locus_tag="OVW45_004551"
     CDS             complement(688..1359)
                     /gene="queE"
                     /locus_tag="OVW45_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCV5528806.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVISRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            1498..>1591
                     /locus_tag="OVW45_004552"
     CDS             1498..>1591
                     /locus_tag="OVW45_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528807.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDN"
BASE COUNT          518 a          325 c          288 g          460 t
ORIGIN      
        1 actcgttagc ctcaaaacaa agacaaattt ccagatttat tacttaatca atgaataaca
       61 agcttaaaaa acaaactatt aacctaaaga aaaaattaac agacattaat tcaatattaa
      121 aaaaataata gattaaaatt tcttaacgat ttaagaatca tacaaataac actttgatta
      181 aatttaattt tttgtatcga taattgtgaa cctctctggc atggagaact attttgaaca
      241 tgaggtgtta cgtggatatt ttgcttatta caagtactgc taatataaaa acttgagaaa
      301 gagataacgg gttatatggt ggtttatccc cgctggcgcg gggaacactc taaacataac
      361 ctattattaa ttaatgattt tttaagccag tcacaatcta ccaactttac agtatcacac
      421 aaacaacaca tccattatgt taaagagtac ttaattcatt gattaaaaag gtaaatattt
      481 aaaataactc tatacaaatt aaaatctacc aaactttacc gcaataattt tcactccagc
      541 gaaaaattaa tgccacagaa tttgtagaga atgctaatga ttagccctgg atgggtaaaa
      601 tggaggagtt ttcagaggcg acaaacaata taaatgagta gaagtcttta caggttccac
      661 ccatttataa cgcttataaa tgtttaatca ggcaatattt agatatttat gtgtttgcat
      721 cgacaaacgc caattacgcg caatgcaggt ttcaatgcac aaacgtgtgg catcatcctt
      781 ttggctaatc ggctgcagtg caatgactcg cggtttatca tcggtcagcg ttgccaatag
      841 ttcatccagt gcttcaatat cgcgtacgcg ccccactgga tgcttgattt cgttggctcg
      901 ctccagtgcc tgtgacaaca cgtcatagcc gccgcgcatg ttcagctttg gcgatacggt
      961 aacccaggta ttcggtgtgc agcgtacctc atgagtacca ctggtttcga tctggcagct
     1021 aaaaccgttc ttttcgagca gatcagtcag tggtagcaaa tcatgaatgc aaggctcacc
     1081 acccgtaatc accacatgcc gcgcggtgta tccctggcga ctaatgacag ccaacaagtc
     1141 ttcactgctc gccgcccccc acttatcact ctctttggtc ttcgccagaa tgctgaaaag
     1201 ggagacttcc cgatcctcaa gcttttccca ggtgtgtttg gtgtcgcacc aggcacagcc
     1261 aaccgggcat ccctgtaaac gaataaaaat ggcggggacg ccggtaaagt aaccctcacc
     1321 ttgcagggtc tggaacatct cgttaatcgg gtactgcata gcattctctg tgaagtggat
     1381 aattgttaat tattgcagat cctgccacaa caatcatgtc ttattaacat tctgttacag
     1441 gcagatttaa agaaaaatgc gaaacatatc gttaataatt aaagggagta acgtattatg
     1501 tcagaagaaa ataaagaaaa tggatttaat catgtcaaaa cattcaccaa aattatattt
     1561 attttttctg tattagtttt taatgataac g
//
LOCUS       DAKISA010000271         1549 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.277, whole genome shotgun sequence.
ACCESSION   DAKISA010000271 DAKISA010000000
VERSION     DAKISA010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.277"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            351..476
                     /locus_tag="OVW45_004553"
     CDS             351..476
                     /locus_tag="OVW45_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCV5528808.1"
                     /translation="MWLPVLFRFLVDSLATLGHRLAASGITSRMLSVIPVLFFWG"
     gene            complement(921..1397)
                     /locus_tag="OVW45_004554"
     CDS             complement(921..1397)
                     /locus_tag="OVW45_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCV5528809.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          357 a          405 c          384 g          403 t
ORIGIN      
        1 aaaaaaacaa ccacttacaa atcggcgtaa aagatctatt ctaggcaagg gtgttgcgtg
       61 caacgcaacg ccctttgggg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctatgcaag cctcgccagc cgtgattttt gccagatttc
      181 gtggttttca cgttcaacag ctccatcgtg tttttgccgt ccgttcaccg ttaaaacgcc
      241 tcagaaatcg ggacaggatg tgtaatcgta taaccgcgca tgcacgccga tacgattacc
      301 acttggtcag ggcttcaccc cgacaccccg catcagtgcg taagtgctca atgtggcttc
      361 cggttctctt ccgcttcctc gtcgattcgc tcgctacgct cggtcatcga cttgcggcga
      421 gcgggatcac ctctcgcatg ttgtcggtga tcccggtgtt gttcttttgg ggatgagtcg
      481 ccagatagcc cctcaaacgc gctcaaaagc tctcagacgc acgaaacggg ttttagatgc
      541 ccgccagggc atggaaggcg cgaaaaacgc ttacagaggc tctcaggagg tcaagcgcgc
      601 cgatacccct aaccaggggc tttcaggtcg ccaggaaccg cgccagcccc ctgcaaagcc
      661 catttgcacg gaacaggcag ccatgcggtt ggtaggtggt cgtagaccgt cggtgttgca
      721 gcgatggggg tctggggttc tccccagacg gtttccggta gcgctcaagg cgctgccgga
      781 accgtggcgg ctcggttttt tctggagaac ggggagcaaa aacacgcaaa ggattttgag
      841 gcggcaaaag agcaatgcag gatagacaat catggtcaga acgttaacac gtagcccctg
      901 attgtccgcc tgccgctagc tcaatttgaa aacatggcca tgattcggcg ctgaatatca
      961 ccgtctttgt tgaccagtgt tttagtgaca aacgctgcat ggtttttcgt agtgttgtat
     1021 cccttccaga cttcatgtgg atcgagggca tacgcacaaa ccttaccagc aacggggatt
     1081 ctaactaatg cattttttga ttctaagtaa ttgagccacc taataattgt gcttctgcta
     1141 actccaagtt tttcagctag gaattgctga tccgcaacga cagcgccaca cgtaggatcg
     1201 atatgctccg ctaaaaatga gtacagcttc agtgctgata tgccttggct atccttcagc
     1261 agctctcgaa cacgttccca accttttggg gatacctgtg taaaccttga atttttcttt
     1321 gcttcttcga gatcctcggt gtttttcttt gcctcatctg aaagttgttt cagtcgtttt
     1381 tctgaatcaa taaacatgat tttttccctc tgaaaatccc atgcactaac gttggtttaa
     1441 atctaagcca tcatgacaca tctgtgcaac accttgttgc gtgttgtcgc atcctggatg
     1501 cgacaacgtt gtgtcatcct ggatgcgaca ggtaaataaa aaacacact
//
LOCUS       DAKISA010000272         1534 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.278, whole genome shotgun sequence.
ACCESSION   DAKISA010000272 DAKISA010000000
VERSION     DAKISA010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.278"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            1..1436
                     /locus_tag="OVW45_004555"
                     /pseudo
     CDS             1..1436
                     /locus_tag="OVW45_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
BASE COUNT          439 a          400 c          372 g          323 t
ORIGIN      
        1 acgaagcaaa aaatgacaaa gtaagaaaac gcctcggtat taaaggcggt ttttactggg
       61 ctgaagcgaa aaaattatcc attgccatct cccgtggtgc tgttgcgatt gacgatgctg
      121 gctacgatga agatgacttt aaaaaacctg ttcgcgtcaa tttgcccgtt gttggtgacc
      181 tcccaccaga aggcgtgttt gatacggaat tctgcaaccg ttacgaaaaa ggcggggaag
      241 atggcatcac aatggtattt atcgcatctt ctccctctgt tcaggacaaa ccagccagca
      301 ctgacaatac caacgtcaac ggcgaagaca tgacagaaat tgaggagaac atgctcctgc
      361 cagtttccgg tcaggaactg cccattcgct ggcttgctca acacggcagc gaaaaaccgg
      421 taacgcacgt ttcacgcgac ggactccagg cattacacat tgctcgggct gaagaactac
      481 cggctgttac tgccctggct gtttcccaca aaaccagcct gctcgacccg ctggaaattc
      541 gcgatctcca caaactggtt cgtgacactg acaaagtttt ccctaatcct ggtaattcaa
      601 gcctgggact gataactgct tttttcgaag catacctgaa cgctgactac accgatcgag
      661 gactgctgac aaaagagtgg atgaagggta atcgtgtttc acacatcact cgcacggctt
      721 ccggtgctaa tgctggcggc ggaaacctca ccgatcgcgg cgaaggtttc gtacacgatc
      781 tgacgtcact ggcgcgcgac gtagccactg gcgtactggc ccgttcaatg gatctggaca
      841 tctataacct tcatccggca cacgctaaac gcattgagga aattatcgct gaaaataaac
      901 cgcccttttc tgttttccgc gacaaattca tcaccatgcc tggcgggctg gattattccc
      961 gcgccatcgt ggttgcgtcc gtaaaagaag caccaattgg gatcgaggtc atccccgcgc
     1021 acgtcactga atatctgaac aaggtactga ctgaaaccga tcatgccaac cctgatccgg
     1081 aaatcgtgga tattgcctgc ggtcgctcct ctgccccgat gccgcagcgt gtaacagaag
     1141 aaggaaaaca ggatgatgaa gaaaaaccgc aaccatcttg cgcaatggca gatgaacagg
     1201 caacggctga aacagtggaa ccggatgcaa ctgaacatca tcaggacacg cagccgctgg
     1261 atgctcagtc acaggtaaat gctgttgatg cgaaatatca gaaactgcgg acagaactcc
     1321 atgaagcccg gaaaaacatt ccgcccaaaa atcctgtcga tgcagacaaa ttactggctg
     1381 cctctcgcgg agaatttgtt gaagggatta gcgacccgaa tgattcgaaa tggattcccg
     1441 gtcatcatat ctcctcaaat gaagtcaaaa aaacggaaaa taaagtgcga cagactgaag
     1501 ataaacagca ccagaacagc gaaccggagg agcg
//
LOCUS       DAKISA010000273         1482 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.279, whole genome shotgun sequence.
ACCESSION   DAKISA010000273 DAKISA010000000
VERSION     DAKISA010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.279"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            1..218
                     /locus_tag="OVW45_004556"
                     /pseudo
     CDS             1..218
                     /locus_tag="OVW45_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343122.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
     gene            128..355
                     /locus_tag="OVW45_004557"
     CDS             128..355
                     /locus_tag="OVW45_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005146028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HCV5528810.1"
                     /translation="MLPENAGSDQHNDSNNETGEDELNWKKQVLIAAVYGLCANPACI
                     AYAPAIPDIAMMIANKLENFGVTSDERLPDF"
     gene            333..629
                     /locus_tag="OVW45_004558"
     CDS             333..629
                     /locus_tag="OVW45_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HCV5528811.1"
                     /translation="MSAYPIFDRIEEMAWARHYQQIVREEKETELADDLEKGLPQHLF
                     ESLCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
     gene            635..>1482
                     /locus_tag="OVW45_004559"
     CDS             635..>1482
                     /locus_tag="OVW45_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCV5528812.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNRKRGLEFSDWAWLKQEWRRFTDRFVNSQAHIIMCGRAG
                     YEYDFFEGDDGKRQLAKTGIKMKAETETGYEPSILIQMEKQMNIETGQVWRTARILKD
                     RSTRIDGQVFSNPTFKNFLPHIEFLNLGGEHLGIDTSRDNSELFANDGTPTWLKEKRA
                     KEIALD"
BASE COUNT          454 a          322 c          376 g          330 t
ORIGIN      
        1 tggcgaggat aactgccctg actgtggcgc ggtgatggga gacgcaactt atcaggaaac
       61 ttttgacgaa aaaaaaccag atgaagcaca ggaagaagag ccgaagaaaa cggaaaaagc
      121 tgatgatttg ttaccggaga acgctggcag cgatcagcat aatgatagca ataatgaaac
      181 tggcgaagat gaactcaact ggaaaaaaca ggtgttgatt gcggcggttt acggtttatg
      241 tgcgaatcct gcgtgcatag cctatgcgcc tgcaatccct gacatcgcaa tgatgattgc
      301 caacaagctt gaaaattttg gagtgaccag tgatgagcgc ttacccgatt tttgatcgta
      361 ttgaagaaat ggcatgggct cgtcactacc agcagatcgt tcgtgaagaa aaagaaacgg
      421 aactggcgga cgacctggaa aaaggtctgc ctcagcacct gtttgaatcg ctctgcatcg
      481 accatttgca acgccacggt gccaataaac aggcaatcag ccacgcattt gatgatgatg
      541 ttgagtttca ggagcgcatg gcagaacaca tccggtacat ggctgaaacc attgcccacc
      601 accaggttga tattgatttg gaggactaaa atctatggcg ctttttcaac gagcaataaa
      661 cacacaggcc tatctaaaag caggaattat ggggtttgct ggcgacggca aaacctacac
      721 agcaagcgaa ctggctattg gtctggtaat gcttatgcgg caacggggaa ttgaagctgg
      781 taaccgcccg gtaatgtttc tggacacaga aacaggctct gactgggtta aaccccgctt
      841 tgatgcggaa aatatcgaat tatttacggc aaaaacccgc tcgtttgttg atctgcttga
      901 agccattaat gaagcggaaa gcagcggttc cgtaatgatc atcgactcca tcagtcactt
      961 ctggaccgga ttatgcgacg agtatgccag acgccgaaac cgtaaacgag gactggaatt
     1021 ttcagactgg gcgtggctca aacaggagtg gcgacgcttt accgatcgat ttgttaacag
     1081 tcaggcgcac attattatgt gcgggcgtgc aggatatgag tatgactttt tcgaaggtga
     1141 tgatggcaaa cgccagttgg caaaaaccgg tataaaaatg aaagcagaaa cagaaaccgg
     1201 ttatgaaccc tccatcctga tccagatgga gaagcaaatg aacatagaaa ccggtcaggt
     1261 gtggcgcacc gccagaatac taaaagatcg ctccacccgt attgatggac aggtattttc
     1321 aaacccaaca tttaaaaact ttcttcctca tatcgagttt cttaacctgg gaggtgaaca
     1381 cctcggcatc gatacctcac gggataacag tgaattattt gccaacgacg gtacaccgac
     1441 atggctaaaa gaaaagcgtg caaaagaaat cgcacttgat ga
//
LOCUS       DAKISA010000274         1456 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.280, whole genome shotgun sequence.
ACCESSION   DAKISA010000274 DAKISA010000000
VERSION     DAKISA010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.280"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            103..315
                     /locus_tag="OVW45_004560"
     CDS             103..315
                     /locus_tag="OVW45_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528813.1"
                     /translation="MLFLNDKEQIIKYKNEMLKINPQITEMVAKYAGCSVEEVERCVE
                     KYFSPSSPSTPSLNELIKLKAKEITK"
     gene            312..674
                     /locus_tag="OVW45_004561"
     CDS             312..674
                     /locus_tag="OVW45_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528814.1"
                     /translation="MIIDLDTLFPVRKTFTDIVSYCTDPFASVESKVFASLPADVECG
                     DLITSTGAKYESGDDIYVVMSEFVTAGENKPVDILRSNAGLVCIKADALNAVSEAAKT
                     ALIKKGFQLEGFNTVFTS"
     gene            693..>1456
                     /locus_tag="OVW45_004562"
     CDS             693..>1456
                     /locus_tag="OVW45_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCV5528815.1"
                     /translation="MILGNDYVDLAPLFQAHSTRNYLLSTLDFTDSVGVTSHKVAVSQ
                     LVESNESLFNKETSRFSSEHNVTKREQGKEWLIEIPYFLREDLIVPADIQGKRKPGTD
                     FQETLTDIYADYVAKHHVAYQRTKESVLAASLFSGKTYTPKTDDVLIEWGKLFNVSAM
                     KATVNASATDTMTVFKQFDQLATDIMEKAQSQAAAVERIVVFCKPAAFNAIRYSVGMV
                     NAFQYASPVDDANVVFNVRSLLPGVTTFTIPGTNIDL"
BASE COUNT          439 a          282 c          297 g          438 t
ORIGIN      
        1 gagttaataa aaggcggctc taaatgctgc cttttatttt gcccgttatg caacttaaaa
       61 gttgtatttc ttactttaaa caaggtaaca aggtttatat aaatgttgtt cttaaatgat
      121 aaagagcaaa tcatcaagta taaaaatgaa atgctcaaaa ttaatccaca aattaccgaa
      181 atggtagcta aatacgctgg gtgttctgtc gaggaagtag aaagatgtgt tgaaaaatat
      241 ttttctcctt cttctccgtc tacaccttcc cttaatgaat taatcaaatt aaaagctaag
      301 gagattacta aatgattatt gatttagaca ctctgtttcc agttcgaaag actttcacag
      361 atattgtaag ttattgtact gatccgtttg cctctgtaga aagcaaggtt tttgcttctc
      421 ttcctgctga tgttgagtgc ggcgacttga tcaccagcac tggcgctaaa tatgaatcag
      481 gagatgatat ctatgttgtt atgagtgaat ttgtaacggc tggagaaaac aaacctgtag
      541 atattttacg tagcaatgct gggcttgtat gcatcaaagc tgatgccctg aatgctgtaa
      601 gtgaagcagc aaaaacagca ttaattaaaa aaggctttca gcttgaaggc tttaacacgg
      661 ttttcacttc ttaataaaag gaataataat aaatgattct aggtaatgat tacgttgatt
      721 tagctccgct gttccaggca cacagcacta gaaactatct gctctctact cttgatttta
      781 ctgattctgt aggggttaca tctcataaag tagctgtttc ccagcttgtc gaaagcaacg
      841 aatctctctt taacaaagag acaagccgtt ttagttctga acacaacgtt actaagcgag
      901 aacaaggtaa agaatggctt atcgaaatcc cgtattttct acgtgaggat ctcattgtcc
      961 cggcagacat tcaaggcaag cgtaagcctg gcactgattt tcaggaaaca ctcactgata
     1021 tctatgctga ctatgttgct aaacatcacg tagcgtatca gcgtacaaaa gagagcgtat
     1081 tggctgcatc tctgtttagc ggtaaaactt acacgcctaa aactgatgat gtgttaattg
     1141 agtggggcaa gctgtttaac gtatcagcta tgaaagccac ggtgaacgct tccgccactg
     1201 acacaatgac cgttttcaag caatttgacc agcttgctac ggatattatg gagaaagcac
     1261 aaagccaggc ggctgctgta gagcgtatcg tagtattctg taagcctgcg gcattcaacg
     1321 ctattcgtta cagtgttggt atggtcaatg ctttccaata tgcaagccct gtagacgatg
     1381 ccaacgtggt gtttaatgtg cgttccctgc taccaggtgt aactactttc accattccag
     1441 gaactaacat tgacct
//
LOCUS       DAKISA010000275         1429 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.281, whole genome shotgun sequence.
ACCESSION   DAKISA010000275 DAKISA010000000
VERSION     DAKISA010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.281"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..119)
                     /locus_tag="OVW45_004563"
     CDS             complement(<1..119)
                     /locus_tag="OVW45_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCV5528816.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQH"
     gene            complement(131..823)
                     /gene="rutB"
                     /locus_tag="OVW45_004564"
     CDS             complement(131..823)
                     /gene="rutB"
                     /locus_tag="OVW45_004564"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCV5528817.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(823..>1429)
                     /locus_tag="OVW45_004565"
     CDS             complement(823..>1429)
                     /locus_tag="OVW45_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCV5528818.1"
                     /translation="KGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFC
                     FGKGVNTPTAFAPTAVRMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGAD
                     EEALSWLTEQSQKDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVP
                     GAEGVLLTFDDFLSGIETFGERIQPLMQCRAHLPALTQEVA"
BASE COUNT          324 a          397 c          396 g          312 t
ORIGIN      
        1 ttatgttgat caaaagccag cgtaccggag acatacacca cgccatcagc cagcgtgccg
       61 ggaacgaagg gggccagcgg tgcgctgctg ccagcgggaa taattacgga ttttggcatc
      121 gttaaactcc ttaagcgata cgagcaaagg acgtgggaga aagcgcgtcg cagaacgttt
      181 cgacgtcgct gacccagcca aaaaaggttt cgatattgaa caacgcggct ttctgtgcaa
      241 attcaggccc cgcctggtgt gttgcgtctt ccagcaccac gccgaaatac tccagaaaaa
      301 agccatcgcg tagcgtcgat tcgacgcaga cgttggtagc gataccggtg aaaaccagat
      361 ggcgtattcc acggctgcgc aaaatgctgt ccagcggcgt attgaagaaa ccgctgtagc
      421 gcggcttcgg cagcacaata tcgccaggct gcggcaccag ttcatccacc agttgataat
      481 cccaggagcc tttcgccagc aatttcccct gcagctgcgg ctgcttacgc atggttttca
      541 gggcgttcga tttatgaaaa ttcggtgagc cgggtccgcc agcttcgaca tactgttcat
      601 cccagccatt ttgaaaccag atgatcagca tccctgccgc tcgcgctgcg gtcacggcgg
      661 tttgaatgtt ggcaatgacc gggcgagtgg ttgagacatc aaacccggcg agatctaagt
      721 agccgcctgg cgtggcataa gcgttttgca tatccaccac gatcagcgca ctttgctgcg
      781 gatcgaaggt aatggcttcc ggtcgagcgg ttaaggtcgt catcatgcca cctcctgagt
      841 cagcgcaggg agatgggcgc ggcactgcat cagtggttga atgcgctcgc cgaaggtttc
      901 gattcccgac agaaaatcat cgaaggtcaa cagcacgcct tcggcaccag gcacgcttgc
      961 gacttcatct aacatgcgcg cgacactggc gtaagaaccg actaatgtcc ccatattgat
     1021 gtttaccgcc gaagtgggat cggccatctg acggacgttg gtgtcagtac cggagcgggt
     1081 atctttctga ctttgttcgg ttagccagct taacgcctct tcatccgcgc ccgctttgta
     1141 gtgttcccat ttggcgcgag cggcatcgtc ggtttcatcg gcaatcacca taaacaacac
     1201 ataagagcca acgtcgcgcc cggtttgctc tgcggcctgt ttcatccgca cagcggtcgg
     1261 ggcgaaagcc gtgggtgtat ttacgccttt gccgaaacag aagttgaaat cggcatactg
     1321 agcggagaac gccatgccag cgtcgctttg cccggcgcag atcactttca tggggacact
     1381 cggttgcgga ctgacgcgac aatcattcat ggtgaaaaaa tcgccttta
//
LOCUS       DAKISA010000276         1394 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.282, whole genome shotgun sequence.
ACCESSION   DAKISA010000276 DAKISA010000000
VERSION     DAKISA010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.282"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..1264
                     /gene="actP"
                     /locus_tag="OVW45_004566"
     CDS             <1..1264
                     /gene="actP"
                     /locus_tag="OVW45_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCV5528819.1"
                     /translation="RYTFADVASYRLKQGPIRILSACGSLVVVALYLIAQMVGAGKLI
                     ELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFGASFMAFMVMKHVG
                     FSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTAGLPHILMRFFTVS
                     DAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAGHLIGGNNMAAVHL
                     ANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVFKKGATEREELRVS
                     KITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLSMYWSKLTTRGAMM
                     GGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAFLGIWFFSATDNSA
                     EGARERELFRAQFIRSQTGFGVEQGRAH"
BASE COUNT          269 a          363 c          392 g          370 t
ORIGIN      
        1 gcgctacacc tttgccgatg tggcctctta ccgtctgaaa caagggccga ttcgtattct
       61 ttcggcctgt ggttctctgg tggtggtggc gctttacctt atcgcccaga tggtgggcgc
      121 aggtaaactg atcgagctgc tgtttggcct taactatcac attgcggtgg tgctggtcgg
      181 cgtgctgatg atgatgtacg tcctgttcgg cggcatgctg gcgaccactt gggtacagat
      241 tatcaaagcc gtgctgttgc tgttcggtgc cagctttatg gcctttatgg tgatgaaaca
      301 cgtcggcttt agcttcaaca atctgttcag tgaagcgatg gcggtacacc cgaaaggtgt
      361 cgacatcatg aagccgggcg ggctggtgaa agatccgatc tccgcactct ctctgggtct
      421 gggactgatg tttggtacgg cgggcttgcc gcacattctg atgcgcttct ttacagtcag
      481 cgatgcccgc gaagcacgta agagcgtgtt ctacgccacc gggtttatgg gctacttcta
      541 tattctgacc tttattatcg gcttcggcgc gatcatgctg gttggtgcga atccggaata
      601 taaagacgcg gcgggccatc tgattggtgg taacaacatg gcggccgttc acctggcgaa
      661 tgcagtgggc ggcaacctgt tcctcggttt tatttcagcg gttgctttcg ccactatcct
      721 cgcggtggtt gcgggtctga cgctggcggg cgcatccgcg gtttcgcatg acttgtacgc
      781 taacgtcttc aaaaaaggcg cgaccgaacg tgaagagctg cgggtatcaa aaatcaccgt
      841 actgatcctc ggcgtgattg cgattatcct cggcgtgctg tttgagaatc agaacatcgc
      901 ctttatggtg gggctggcgt ttgccatcgc ggcgagctgt aacttcccga tcattctgct
      961 ttctatgtac tggtcgaaac tgaccacgcg tggcgcgatg atgggtggct ggctggggct
     1021 gattaccgca gtagtactga tgatcctcgg cccgacgatt tgggtacaga tccttggtca
     1081 cgaaaaagcc atcttcccgt atgaataccc ggcgctgttc tctatcaccg tggcattcct
     1141 cggcatctgg ttcttctcgg caaccgataa ctcagcggaa ggggcgcgcg agcgtgaact
     1201 gttccgcgcg cagtttatcc gctcccagac cggctttggc gttgagcaag gccgcgcaca
     1261 ttaatctctc cccttccccg gtcgcctgac cggggaatac tcttcctctc cagcatgcat
     1321 caccttttcc caaaatatta aacaaataaa ctcattaaaa aatgagcgat ttttgacagt
     1381 cgtagaaaat gata
//
LOCUS       DAKISA010000277         1384 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.283, whole genome shotgun sequence.
ACCESSION   DAKISA010000277 DAKISA010000000
VERSION     DAKISA010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.283"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..1339)
                     /locus_tag="OVW45_004567"
     CDS             complement(<1..1339)
                     /locus_tag="OVW45_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HCV5528820.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTH
                     AGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGY
                     YSLGLLNAWSLAGEHRHWMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGN
                     EVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSR
                     LTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLM
                     TLQGASPGRIPELMRDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQS"
BASE COUNT          281 a          391 c          357 g          355 t
ORIGIN      
        1 ctgactggct ctttttcggg gctgtggggt atttccaggg cctctccttt accactctcg
       61 ggaaggccct ttcccttctc gtcggtaatt tcacaagttg tcccatactt gcaagatcgc
      121 gcatcagctc cggtatacgt cccggtgaag cgccctgcaa tgtcatcagc attctcatca
      181 ccattccgca tgattctgag aaactcagtt gattcggcca gtaacctttc agatgttccg
      241 ccattttaat catctgatat ctcaccagat tataagccag taagacaccc cacagctctt
      301 gctccacaag ctccggcttt ttacttctca gcgtcagcct gctcagttgc atcgtctgtt
      361 ttatctccct gtatcccagt tcgatttccc agcgatgact gtacagatcc gccatttctc
      421 ctccggggaa gcgcatggcg tccgtcatcg acgtcagcag atggcagact tttcctttgc
      481 gcgtcacggt cagcaggcgg gctgtcactt catttcccag tcccggccac ttttttcgtg
      541 cctgcgggct ggttttcagc ttcaccagat gatcgccttt acccagtttt ctgagctctt
      601 catattgcgc tccctttctg agaggtatca tccagtggcg gtgttctccc gccaggctcc
      661 aggcatttaa cagtcccagt gagtaataac ctttatccat taacgtcaga gtgttatcgc
      721 cggtttgttc tataagttgc tcagcaagct cattttcgct gttcttcatc gtgccgaagg
      781 ctgcagccgt cagcagatgg ctggtcagtt ccatctggca gaccattttg acctgcgggt
      841 agagcgccgg gttcccggca tgtgtctggc gggggaaggc tgcatcgttc tctggtgtat
      901 ccggtgtgcg ccagaacaca ccatcgatgg ccagcagggt caggccgcac cagtgcggat
      961 gcggcgtggc gttatgccag agctgcgctg ttttcgtgaa cacgcggcgg acagcctcac
     1021 ttcccaggcg ctggcgggcc tgaataacgg cactgggggc aacgaagggg cgattgcccg
     1081 gcagcatgat gtccaggcga ttcacaatct ggtgaagagg ttctttacgc tcaagcgcca
     1141 tgccaacaat acaccagacc atcatttcga ggggaagacg gcgcttgcgt agcgttacag
     1201 tacctgattc ggcaaggcaa cgagagatga gttcggggtc gaggtaatcc cccagagaag
     1261 tcagtgggtt acgcagagaa tcgtaacggg ataccagatc aagagcctgt ccaatgtgca
     1321 taaaaaaatc cggaaacaag tgagcgtttc cggattctta cacagccact ggatcggtca
     1381 actg
//
LOCUS       DAKISA010000278         1382 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.284, whole genome shotgun sequence.
ACCESSION   DAKISA010000278 DAKISA010000000
VERSION     DAKISA010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.284"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            4..984
                     /locus_tag="OVW45_004568"
     CDS             4..984
                     /locus_tag="OVW45_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCV5528821.1"
                     /translation="MVGVPVPDNIHPMIKDEAGVWSWRTPVLKGNLYEYFFNVDGVRS
                     IDIGTAMTKPQRQVNSSMVLVPGSYLDTRSVAHGDLIAITYHSNALQSERQMYVWTPP
                     GYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPDTETDA
                     KGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALAGLSQG
                     GYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTVVVGDK
                     DVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            999..>1382
                     /locus_tag="OVW45_004569"
     CDS             999..>1382
                     /locus_tag="OVW45_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCV5528822.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGA"
BASE COUNT          360 a          316 c          365 g          341 t
ORIGIN      
        1 ttagtggtgg gtgttccggt tccggacaat attcacccga tgatcaaaga cgaagcagga
       61 gtctggtcgt ggcgcacgcc tgtcctgaaa ggcaacctgt atgagtattt tttcaatgtt
      121 gatggtgtac gcagcattga tataggcacc gcaatgacta agcctcagcg ccaggttaac
      181 tccagtatgg ttctggtgcc aggcagttat ctggatacgc gttctgttgc gcatggtgat
      241 ttgatcgcca taacttacca ctccaacgca ttgcaatctg aacgtcagat gtatgtctgg
      301 accccgccag gatacaccgg catgggcgag cctttgccag tgctctattt ctatcacggt
      361 tttggtgata ccggacgttc cgctatcgat caggggcgta tcccgcaaat catggataac
      421 ctgctggctg aagggaaaat taaaccgatg ctggtggtga tcccggatac cgaaaccgat
      481 gcgaagggca ttattcccga agatttcgtg cctcaggaaa gacgtaaagt cttttatccg
      541 ctgaatgcta aagcggcaga tcgcgaactg atgaacgata ttatcccgct gattagcaag
      601 cgttttaatg tccgtaaaga tgccgatggt cgcgcgctgg cagggctttc acaaggcggg
      661 taccaggcgc tggtttccgg aatgaatcat ctggaaagct ttggctggct ggccacattc
      721 agtggtgtta ccacgacaac cgtaccggat gaaggtgtcg cggcccggct gaacgatccg
      781 gcagctatta accagcaact acgtaatttt actgtggtcg ttggtgataa agatgtcgta
      841 accggcaagg atatcgccgg gctgaaaact gagcttgagc agaaaaaaat taagtttgat
      901 taccaggaat atcccggcct gaaccatgaa atggatgtct ggcggcctgc ctatgcagcc
      961 ttcgtacaga aattatttaa ataaaataag gcattaagat gaaattaatc attaccgaag
     1021 attaccagga aatgagccgt gtcgcggcac accatctgct tggttatatg tcgaagacgc
     1081 gtcgtgttaa cctggcaatt accgccggta gcacgcccaa aggcatgtat gaatacctca
     1141 ccaccctggt taaaggtaag ccctggtacg ataactgcta tttctataat ttcgatgaaa
     1201 ttccctttcg cggcaaagag ggagaaggcg taacgattac caatctgcgt aatctgtttt
     1261 tcacccctgc ggggatcaaa gaagagaata tccagaagct cactattgat aactaccgcg
     1321 agcatgatca gaaactggcg cgtgaaggcg gcctggattt ggtggtgctg ggattaggtg
     1381 ca
//
LOCUS       DAKISA010000279         1353 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.285, whole genome shotgun sequence.
ACCESSION   DAKISA010000279 DAKISA010000000
VERSION     DAKISA010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.285"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            230..1282
                     /locus_tag="OVW45_004570"
                     /pseudo
     CDS             230..1282
                     /locus_tag="OVW45_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          360 a          348 c          372 g          273 t
ORIGIN      
        1 ccttcagtag cggagcagaa aggacatctg atggaaatgg aagccacgca agcaccttaa
       61 aatcaccatc atacactaaa tcagtaagtt ggcagcatta cctacaatcg tcagcgtcgt
      121 cactctcgtc tggggaatat ctccccggca gccttcaggg aaaaatatca tcagatgact
      181 gcttaaaaaa agaacaaatg gtagtgtccg ctattgccag tacacctcaa tccgcactgt
      241 tcgggtaaaa aaagcctgta caaaatgtga ctgcatcatt gaagcgccgg ctccgtcccg
      301 tccgatagag cgcggcatcg caggtcccgg cttgcttgcc cgcgtgttaa cgggaaaata
      361 ctgcgaacat ctgccactgt accgtcagag tgaaatcttt gcccgtcagg gcgtcgagct
      421 gagccgtgca ttactctcca actgggttga cgcatgctgc cagttaatga cgccgctgaa
      481 tgatgccctg taccgctatg tgatgaatac ccgcaagctt cacactgacg acacaccggt
      541 aaaagtgctg gcaccgggca gaaaaaaggc aaaaacagga cgcatctgga catatgtccg
      601 ggatgagcgg aatgcgggct cgtcagagcc accggcggtc tggttcgcct actcaccgga
      661 caggcaggga aaacatccgg tacaacacct tcgtcccttc cggggtatcc tgcaggcgga
      721 tgcgttcagc ggttacgatc ggctgttcag tgccggacgt gaaggcggtg cactgacaga
      781 agttgcgtgc tgggcccatg cccggcgaaa aatccacgat gtatacatca gcagcaaaag
      841 tacgacggca gaagaagccc tgaaacgcat cagtgaactg tacgccatcg aggatgaaat
      901 acggggctta ccagagtctg aacgtcttgc cgtcaggcag cagcgaagca aagcgttact
      961 gacgtcgctg aatgaatgga tggtggagaa gaatggtacg ctgtcgaaaa aatccagact
     1021 gggcgaagcg ttcagctatg tactgaatca gtgggatgcc ctctgttatt acagtgatga
     1081 cggtctggcg gaggcggaca ataacacagc agaaagagcg cttcgtgccg tctgtctcgg
     1141 gaaaaagaat tacatgttct tcggcagcga tcatggaggc gatcgcggag ctctgctgta
     1201 cggactaatc gggagctgtc ggttgaacgg tatcgatcca gaagcgtatc tgcgccatat
     1261 cctgagcgta ctgccggaat gaccttccaa ccgagttgac gaactgctgc catggaacgt
     1321 agttctcacc gataaataag cgtcaatacg gcc
//
LOCUS       DAKISA010000280         1341 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.286, whole genome shotgun sequence.
ACCESSION   DAKISA010000280 DAKISA010000000
VERSION     DAKISA010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.286"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            80..>1341
                     /locus_tag="OVW45_004571"
     CDS             80..>1341
                     /locus_tag="OVW45_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCV5528823.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRAFSAFLDRLSDTVSARNTSGFREQVAAWLEKLSASAELRQQSFTV
                     AADATESCEDRVALTWNNLRKTLLVHQAS"
BASE COUNT          392 a          330 c          235 g          384 t
ORIGIN      
        1 ggcgaatatt ctgaatcacc ctcctcatta ggagagatac attaaataca atttattcac
       61 aatctaattc catatccata tgcgagaaat taatatgctc agaaatattt catcctgttt
      121 atttccacat atcagcacaa ttacatcccc caaccattat ttgtccgaat gggatgattg
      181 ggagaaacag gggttaccgg aagaacagcg tactgaggcg gtaagaagac ttcgtgcatg
      241 tcttacctct aaggggcata aactggacct gcgagccttg gcgctttcct cgttacctgt
      301 actccctgct tgcattaaaa agcttgatgt gagctgtaat aaattaacca tccttactga
      361 tttacctgaa aatattaaag aacttattgc aagagataat tttttaacac atatatctgc
      421 attaccacat tatctaataa ctttggatgt gtccgaaaat caattagaga atctgccgtt
      481 attaccagac accatcaaat cactaagcgc agagtataat aggttatcca cactgccttc
      541 attacccttg aatttaaaaa aacttgaggt taggaacaac gaactgcaaa ctcttccatc
      601 tctgccttct aatcttaaga tacttaaggt tgcgcacaac catcttactg aactgccccc
      661 tttacctagg agactgcaac ttctttttgc atatagcaat agattaagca acttaccaaa
      721 catccaagaa aatattatca tgagaagatt tttttatttt gaaaacaacc aaataactac
      781 aatcccgaca aatctttttc gtttagatcc tcatataact attgagattg caaataaccc
      841 cttatcagat caaactctgc tattcttaat acagcaaact tcggttccaa attttaacgg
      901 gcctcagttt cgtatttccc tgtcagacca aaacagactg tttttacgcc agatgttgcc
      961 gcaaaattta cattcgcgcc atatcagagt catcactgaa ggggggcaga actttcagat
     1021 cccccctctt cccgaaactg tggcagcctg gtttcctgaa gcagatcgtc gggaggtttc
     1081 tacacaatgg acttcttttt ccaccgagga gaattcccgg gcattctccg cgttccttga
     1141 ccgcctttcc gataccgtct ctgcacgcaa tacctccgga ttccgtgaac aggtcgctgc
     1201 atggctggaa aaactcagtg cctctgcgga gcttcgacag cagtctttca ctgttgctgc
     1261 tgatgccact gagagctgtg aggaccgtgt cgcgctcaca tggaacaatc tccggaaaac
     1321 cctcctggtc catcaggcat c
//
LOCUS       DAKISA010000281         1296 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.287, whole genome shotgun sequence.
ACCESSION   DAKISA010000281 DAKISA010000000
VERSION     DAKISA010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.287"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..485
                     /locus_tag="OVW45_004572"
     CDS             <1..485
                     /locus_tag="OVW45_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCV5528824.1"
                     /translation="AAGASAPQILDQFLDEKEGNHTTAYRDGSGIWTICRGATMVDGK
                     PVFPGMKLSKEKCDQVNAIERDKALAWVEKNIKVPLSEPQKAGIASFCPYNIGPGKCF
                     PSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQVFRRDQESALACWGIDR
                     "
     gene            707..820
                     /locus_tag="OVW45_004573"
     CDS             707..820
                     /locus_tag="OVW45_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528825.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRIIAIAVLIWAIRWW"
     gene            822..1289
                     /locus_tag="OVW45_004574"
     CDS             822..1289
                     /locus_tag="OVW45_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCV5528826.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYRDNAITYKAQRDNKASEL
                     KLANATITDMQVRQRDVAALDAKYSRELADARAENETLRADVAAGRKRLRINATCPGS
                     VREAPTTSGVDNATGPQLADTVTRDYFILRERLMTMHKQLEGAQDYIRTQCLK"
BASE COUNT          327 a          295 c          377 g          297 t
ORIGIN      
        1 ttgctgcagg cgcgtctgct cctcaaatac ttgatcagtt tctggatgaa aaagagggta
       61 accacactac ggcataccgc gatggttccg gcatctggac catctgtcgg ggggccacga
      121 tggtggatgg aaaacccgtt tttcccggta tgaaactgtc gaaggaaaaa tgcgaccagg
      181 tcaacgccat tgagcgtgat aaggcgctgg catgggtgga gaaaaacatc aaagtgccat
      241 tgagcgaacc ccagaaagcg gggatcgcgt cattctgtcc gtacaacatt ggtcccggta
      301 agtgtttccc gtcgacgttt tacagacgaa ttaatgctgg tgatcgcagg ggagcatgcg
      361 aagcgattcg ctggtggatt aaggacggtg gcagagactg ccgtattcgc tcaaataact
      421 gttacggtca ggtattccgt cgtgaccagg agagcgcgct ggcgtgctgg ggaatcgaca
      481 gataagcaga atattttgct gaaaaatgag gttggccaac gcgggcggat aacacgaagt
      541 cctgtgaact ggcaaaacct aagtgaataa aagtaaaaac cccgtttgtt ggcagcaagc
      601 ggggttttgt gttttctgac cttgagtaag gcaagggaga aattatggtg agggaggtgc
      661 tttccctgtg aggaagtata aaagattctt tctgaggttg tccattatga aaggcattga
      721 agtggagacg ccagccagtc tggatttaac aagagcggca gcttttgcca ttcgtattat
      781 agccattgct gttctgatct gggcaatccg ttggtggtga aatgaaccgc gttctgtgtg
      841 tggtcatcat tgctctgctg gtggcctgtg gtgcgcttag tctggggctg aatcattacc
      901 gtgataacgc gattacctac aaagcgcagc gcgataacaa agccagtgaa ctgaagctgg
      961 cgaacgcaac cattactgat atgcaggtgc gccagcgcga tgttgctgcg ctcgatgcaa
     1021 aatactcgag ggaattagcc gatgcgagag ctgaaaatga aactcttcgc gctgacgttg
     1081 ccgctggtcg taagcgcctg cggatcaacg ccacctgtcc aggctccgtg cgtgaagccc
     1141 ccaccacctc cggcgtggat aatgcaaccg gcccccaact ggcagacacc gttacacggg
     1201 attatttcat cctcagagag cggctgatga cgatgcacaa gcaactggaa ggggcacagg
     1261 actatatccg cactcagtgc ctgaaataag ttttgt
//
LOCUS       DAKISA010000282         1229 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.288, whole genome shotgun sequence.
ACCESSION   DAKISA010000282 DAKISA010000000
VERSION     DAKISA010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.288"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            15..1073
                     /locus_tag="OVW45_004575"
     CDS             15..1073
                     /locus_tag="OVW45_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000035736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HCV5528827.1"
                     /translation="MAQAGIRPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTV
                     FTRYQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACH
                     SLIPEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFR
                     DHNITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKS
                     SEWGWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDH
                     LVQVNEAIADGVDIMEYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKK
                     SFGWYAEVIKTRGLSLKK"
BASE COUNT          335 a          258 c          313 g          323 t
ORIGIN      
        1 ggctgtttga tgaaatggcg caggcgggga tcaggccgct ggtaacgtta tcccattacg
       61 aaatgccata tgggctggtg aaaaactacg gcggttgggc taatcgggcg gtcatcgatc
      121 acttcgagca ttacgcccgc acggtcttta cccgctacca acataaagta gcgttatggc
      181 tgacgtttaa tgaaatcaat atgtcgttac acgcgccatt cacgggcgtg gggctggcag
      241 aagagagtgg cgaggcggaa gtttatcagg cgatccatca tcaactggtt gccagtgcgc
      301 gggcagttaa agcctgtcat agcctgatcc ccgaagcgaa aatcggcaat atgcttctcg
      361 gtgggctggt ttaccccctc acctgccagc cacaggatat gttgcaggcc atggaagaga
      421 accggcgctg gatgttcttt ggtgatgttc aggcgcgtgg ccagtatccc ggctatatgc
      481 agcgtttctt ccgcgaccac aatatcacca ttgagatgac tgaaagtgac gcagaagatt
      541 taaaacatac tgtagatttc atctctttta gttattacat gactggttgt gtttcccacg
      601 acgaaagcat taataaaaat gcgcagggca acatactgaa tatgatcccc aatccgcatc
      661 tgaaaagttc agagtggggg tggcaaattg atccggttgg attacgggtt ctgttaaata
      721 cgctttggga tcgttatcaa aaaccgttat ttattgtcga gaacggatta ggcgcaaaag
      781 acagcgttga agcggatggt tcgatacagg acgattatcg aattgcctat ttaaacgatc
      841 acctggtaca ggtaaatgaa gcgattgccg atggtgtgga tattatggag tacaccagtt
      901 gggggccaat tgatttagtc agtgcatctc attcacaaat gtctaagcgc tacggcttta
      961 tttatgtgga tcgtgatgat aatggcgaag gaagcctcac aagaacgcgt aagaaaagct
     1021 tcggatggta tgcagaagtg atcaaaacgc gggggctgtc attaaaaaaa taaccaataa
     1081 agcaccttaa ttatcgtcgc attcagaaca gtctggatgc gatacgttaa ttctttcttt
     1141 gcaccataaa gggatattat gtttagacga aatcttatta cctctgccat cttattaatg
     1201 gcaccgttag ccttttctgc acaatcatt
//
LOCUS       DAKISA010000283         1219 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.289, whole genome shotgun sequence.
ACCESSION   DAKISA010000283 DAKISA010000000
VERSION     DAKISA010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.289"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..560
                     /locus_tag="OVW45_004576"
     CDS             <1..560
                     /locus_tag="OVW45_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCV5528828.1"
                     /translation="RFSQLESALNTQKNSIPALEKEVKALDKQMVAAQKAADAYWGKD
                     ANGKQMTREEAFKKIHQQRDEFNKQNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVP
                     IQQKRDFDINEQRRQTFLQSQKLSRKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMK
                     IDDINQANERWKKDTEQLRRNGVIK"
     gene            complement(616..1171)
                     /locus_tag="OVW45_004577"
                     /pseudo
     CDS             complement(616..1171)
                     /locus_tag="OVW45_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
BASE COUNT          420 a          238 c          281 g          280 t
ORIGIN      
        1 atcgctttag tcagcttgaa tctgcgttga atacccagaa gaactcgatt cctgcgctgg
       61 aaaaggaagt gaaagcgctt gataagcaaa tggtggcggc gcagaaagca gcggatgcat
      121 attggggaaa agatgcaaac ggtaagcaaa tgacccgcga agaagccttt aaaaaaattc
      181 accaacagcg tgatgagttt aataaacaga acgatagcga ggcgttcgcg gttaaatatg
      241 acaaagaggt ttatcaaccg gcgattgcgg catgtcataa acagagtgaa gagtgttatg
      301 aagtgccgat tcagcagaaa cgagatttcg atatcaacga acaacggcga cagacttttc
      361 tgcaatcaca aaaactcagc cgtaaattgc aggatgactg ggtaacgctc gaaaaagggc
      421 aatatccgtt aacgatgaaa gtttcagaaa taaacagtaa aaaagtcgcg attttgatga
      481 aaattgacga tatcaaccaa gctaatgaac gctggaaaaa agataccgaa caactgcggc
      541 gcaatggggt gattaagtaa ctcatgttaa gtgcctttgc cggataatat aaaaagttat
      601 ccggcaaatt attttttaca ttagccctag tagctgtgga atgaacaagg atatttgcgg
      661 aatgtaagtg atcatcatca acgctactag caatgctagc ataaaatggc agcaatggtt
      721 tgattaaatg ttgaatcttc acacccgaga tagaacaacc aacaaatagc gcactaccca
      781 ccggcggtgt taacagccca atacaaaggt tggccaccat cataataccg aaatgtactg
      841 ggtccattcc cagctcttgt gcaatcggca agaagatcgg tgtaaagatt agtacagccg
      901 gtgtcatgtc cataaagatg ccgacaatta ataagacgat attaatcagc agcaatatga
      961 tcagtgggtt atcagaaatc cccatcaatg cgtcgctgat catataagga atatcagcat
     1021 tagtcattgc ccaggacatg cccacggaaa aaccgatcaa cagtaataca atcgaagtgg
     1081 taacaaccga ctccagaatc agtttgggta aatggcgcca ttttacttcc cggtaaatca
     1141 gcaccgagag aataaatgtg tagacaacgg cggtgatgct gccaacttac tgatttagtg
     1201 tatgatggtg tttttgagg
//
LOCUS       DAKISA010000284         1212 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.290, whole genome shotgun sequence.
ACCESSION   DAKISA010000284 DAKISA010000000
VERSION     DAKISA010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.290"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            229..>1212
                     /gene="gspA"
                     /locus_tag="OVW45_004578"
     CDS             229..>1212
                     /gene="gspA"
                     /locus_tag="OVW45_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HCV5528829.1"
                     /translation="MSTRREVILSWLREKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQGIELVSTGRLTEH
                     ELKASILEGQNIDQPDLLLTSRVLKRIALLCRGDRRKLALAGETIRLLQQAEQTSVFT
                     AKQWRMIYRILGDNRPRKMQLAVVMSGTIIAITCGWLLLSSFTAPFPVPAWLVPVTPV
                     VKQDMTKDIAHVVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLACKSGN"
BASE COUNT          327 a          249 c          310 g          326 t
ORIGIN      
        1 catctttatt atgattcgcc agatggaagg ggaaacgtag tgtgggcacg atgtatgttc
       61 taataaaata gattgggtgg gttgtatatt gacgttagta taaatagtat taatgccgct
      121 tgcatgaatc aagcaaaatt tatttatatt agtaatatgt atttaattaa taaatacata
      181 cattattttt atgattaagg ataatcaata attaaaacag gaagttctat gtctacgaga
      241 agagaagtta ttctttcctg gttgcgtgag aaacgtcaga cctggcgtct gtgctatttg
      301 ttgggtgagg ctggaagtgg aaaaacctgg ctggcgcagc aactgcaaaa agataaacat
      361 cgccgtgtga ttactttaag cctcgttgtt tcctggcaag gtaaggccgc atggatcgtt
      421 accgacgata acgcggctga acagggctgc cgtgacagcg cctggacgcg agatgagatg
      481 gcggggcaat tactgcatgc gcttcatcga actgatagtc gctgccctct tattattatt
      541 gaaaatgctc acctgaatca ccgccgcata cttgatgatc tccaacgcgc gatatctctt
      601 attcctgacg gtcaattttt gctgatcggc agacccgatc gaaaagtcga acgtgatttt
      661 aaaaaacagg gcattgaact tgtctcgaca ggacgtctga cggagcacga acttaaggca
      721 agcatccttg aaggtcagaa tattgaccaa cccgatctcc ttttaacctc cagagttctg
      781 aaacggatag ctttattatg tcggggcgat cgcagaaagc tggcgcttgc cggcgaaaca
      841 attcgcttat tacagcaagc cgagcagacc agcgttttca ctgcaaaaca atggcgaatg
      901 atttaccgca tattgggtga taaccgcccc cggaaaatgc agcttgccgt ggtaatgagt
      961 ggaaccatta tcgctattac ctgtggttgg ctactgctgt cctctttcac agctccgttc
     1021 cccgttcctg cgtggctggt acccgtaacg ccagtagtta aacaagatat gactaaggat
     1081 attgctcatg tggtgatgcg cgatagtgag gcgctaagcg tgttatacgg cgtatggggg
     1141 tacgaggtcc cagctgattc ggcctggtgc gatcaggcag taagagcggg gctggcatgt
     1201 aaaagtggga at
//
LOCUS       DAKISA010000285         1185 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.291, whole genome shotgun sequence.
ACCESSION   DAKISA010000285 DAKISA010000000
VERSION     DAKISA010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.291"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(69..707)
                     /locus_tag="OVW45_004579"
     CDS             complement(69..707)
                     /locus_tag="OVW45_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCV5528830.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
     gene            complement(725..>1185)
                     /locus_tag="OVW45_004580"
     CDS             complement(725..>1185)
                     /locus_tag="OVW45_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCV5528831.1"
                     /translation="LNAMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEAT
                     DYYKTPRGQQPGGATKMLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRD
                     GLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
BASE COUNT          309 a          275 c          244 g          357 t
ORIGIN      
        1 gttcacctta ataagtttaa taaatcgtac tgcacatagt acattacgat ggtaactatt
       61 taattggttt acatgaataa tggcttgtca ccatcagtca cgggttgatt gatacctata
      121 ttctcaccaa tgtgtttctc aggtttatcg atgatatctg tgattaatgc tgcgacactc
      181 ttacgtgata cgatcgtacc tttgaatggt tcattgcgtg aggtaagctc ataatcaata
      241 atatcttcat cagtcagcca ggcagggcga agaattgtat attccagtcc agaagcctca
      301 attgcatccg cagccctgcg gaatggtttt aggggctcac caataactgc attattccat
      361 tcgccaaatt ttcctggaac ttcatcataa ataccgaggg aaagaacaaa gatcagacgt
      421 ttaacatcac aggctttcat cgcagcgatc actgaatttg cctgaatatc taaatcttcc
      481 cccgtaaggt tggcatacac aacgtcctgt ccctgcatgg cctgcttcag tgctgcatga
      541 ttcagtacat ctcccataat aatcttgcta ttagttggat agggtttgtg gatttttgct
      601 ggctgacggg caaataaagt ttgtttaatg gtttgtttat ctgccagttg attaatcaca
      661 tggcgggcta tctgaccgcc agcaccaaga atcagtacat ttttcataat atttctcatg
      721 aaacttatcc cagatgggtg ttgaaaaaag gaataacttt ttccagcgcc tcctgaactg
      781 cttccggttt gtcatacagt tcataatgtg agaaatcggg taatgaaact aactgacgat
      841 cctgcgaaac catcgcccgg ccatagactt ccagtccatc acgataagca ccgaaggcac
      901 cgactttttc accaactact accatcaccg gctgagtcag taacacttca gtaaatgcga
      961 aggcatccca ggccagcgtt ttctgtgcat gtgagaacaa catttttgtc gcacctcccg
     1021 gttgttgccc gcgtggtgtt ttatagtagt cagttgcttc ataaacatcg cgttcggtca
     1081 gtccatgggc ttttgctgca tccaggctgg cgggaagtaa ttcgttgatc tgtaattctc
     1141 caccacgagc ctctttggtt cgttgcgacg ccattgcatt caatg
//
LOCUS       DAKISA010000286         1177 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.292, whole genome shotgun sequence.
ACCESSION   DAKISA010000286 DAKISA010000000
VERSION     DAKISA010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.292"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            312..545
                     /locus_tag="OVW45_004581"
     CDS             312..545
                     /locus_tag="OVW45_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HCV5528832.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALVQKGSGNFSAWVIEA
                     CRRRLCSEKRVSPEANKEKSDITELLRKQVRPD"
     gene            603..1013
                     /locus_tag="OVW45_004582"
     CDS             603..1013
                     /locus_tag="OVW45_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HCV5528833.1"
                     /translation="MAQVAIFKQIFDKVRNNLNYHWFYSELKRHNVSHYIYYLATENI
                     HLVLENDNTVLIKGQGKVVNVRFSKNKCLIEATLKGFKSGELSFYEYRKNLATAGVFR
                     WITNIHENKRYYYTFDNSLLFTENIQNTTQIFPH"
BASE COUNT          405 a          258 c          219 g          295 t
ORIGIN      
        1 ttcttttcag ggggtgtttt aaataaaaac attaggttac ggcgaagaag aacggaaacg
       61 ccttaaaccg gaaaattgtc ataaatagcg aaaacccgcg cgccttccgc cccgtaacct
      121 gtcggatcgc cggaaaggac ccgcaaaatg ataataatta tcatctacat gtcacaacgt
      181 gcatctacgc catcaaacca cgtcaaataa tcaattatga cgcaggtatc gtattaattg
      241 atctgcatca acttaacgta aaaacaactt cagacaatac aaatcagcga cactgaatac
      301 agggcaacct catgtcaacg aagaacagaa cccgcagaac aacaacccgc aacatccgct
      361 ttcctaacca aatgattgaa caaattaaca tcgctcttgt tcaaaaaggg tccgggaatt
      421 tctcagcctg ggtcattgaa gcctgccgcc ggagactgtg ctcagaaaaa agagtttcgc
      481 ctgaagcaaa caaagaaaag agtgacatta ctgaattgct cagaaaacag gttagaccag
      541 attgaagcaa tttagataat cgtgcagact acgccccctc atatcacatg gaaggtacta
      601 caatggctca ggttgccatt tttaaacaaa tattcgataa agtgcgaaat aatttaaact
      661 atcactggtt ttattctgaa ctaaaacgtc acaatgtctc acattacatt tactatttag
      721 ccacagagaa tattcatctt gttcttgaaa acgataatac ggttttaata aaaggacagg
      781 gtaaggttgt aaatgtaaga ttttcaaaaa ataaatgcct tatagaagcc accttaaaag
      841 gattcaaatc aggagagtta tcattttacg aatacaggaa aaatcttgct acagcagggg
      901 ttttcagatg gattacaaat atccacgaaa acaaaaggta ttactatacc tttgataatt
      961 cattactctt tactgagaac atacagaaca ctacacaaat atttccgcac taaatcataa
     1021 cgtccggttt cttccgtgcc agaaccggac tcgctggcat gatgaaatat gtgtacccgg
     1081 taaccccggt gtgcatcgtt tttgattatt cccgcacact cgcgcagaag gagttccccg
     1141 tcgggctacg gtctctgtta atacgggaat acggcga
//
LOCUS       DAKISA010000287         1172 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.293, whole genome shotgun sequence.
ACCESSION   DAKISA010000287 DAKISA010000000
VERSION     DAKISA010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.293"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..806)
                     /locus_tag="OVW45_004583"
     CDS             complement(<1..806)
                     /locus_tag="OVW45_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCV5528834.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGRGNPKSFTAHNRAQAVFLC
                     VKHSHIRIMVGRAGQPQGWPGSVMTGISTPVRLTTLMVVENLGGELIKFIAEAAIMAT
                     ILTLSHPDVTIENGRAVTTSVAIAEFFGKRHERVLDKIRNLDCSAKFTEHNFVSSEYT
                     DSTGRKLPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKM
                     IPGDGRTLVVHFDKFGNVEFTETVPDGALVCPLETFRFYLEKQGWTLVNRG"
BASE COUNT          287 a          305 c          284 g          296 t
ORIGIN      
        1 ccccggttaa caagagtcca tccctgtttt tccagataaa accggaaagt ctccagggga
       61 cagaccagtg cgccatcagg aacggtttcg gtgaattcga cattgccgaa tttgtcgaag
      121 tgaacaacca gagtgcgacc atcacccgga atcatcttgt cagcaggtgg ggtgttattc
      181 tggcgcagtt cggcctccat gcggtcgaac tcagcaatgt atgcctcttt gaatgcggcg
      241 gcttttttgc ccgtgaagcc catcaccagg aaaacgaagc cgtttttggt gatttggtac
      301 attgggagtt tgcgcccggt tgagtcggtg tattcgctcg acacaaaatt gtgctcagtg
      361 aattttgctg aacagtccag attgcgaatt ttatccaaca ctcgttcgtg gcgtttgcca
      421 aagaactcgg cgatcgcaac agaggtagtg acagcgcgac cattttcgat ggttacgtca
      481 gggtgagaaa gggtaaggat agtagccatg atggcagcct ccgcgatgaa tttgattaac
      541 tcaccaccga ggttttccac gaccataagg gtggtgagac gtacaggggt ggaaataccg
      601 gtcatcacgg aacccggcca gccttgcggt tgccctgcac gccccaccat aatgcgaatg
      661 tggctgtgct taacgcataa aaaaaccgcc tgagcgcggt tatgcgccgt gaatgatttc
      721 gggtttccac gcccggcacc cgttttatga ggtgcaggtg cactataatt ccacccgttc
      781 tggttttcaa tagctacatt caacattttc tctcaccttt catcaccgaa gtgaattttg
      841 ttgatgcggt gcctggtgcc tccaggtgac gttaaccagt taacaattac agttggcttt
      901 cccacccaaa ccaataagga ctaacatgac ttttaactgt gccgcgtgcg catagccgca
      961 ttcaccgcat cacaaaattc actttaaaaa gggcggacat cagccagcaa ttaaaccgat
     1021 gccgccaact ggtacttcac acagcaatgt cgttatttac aaccggaagc gcactcccac
     1081 catttaaatt tcacagacaa gaccgactct ttatggatac cggaaatgcg ccttcgtgtt
     1141 gtgcccggtt ttatttcacc acctccgggc tt
//
LOCUS       DAKISA010000288         1158 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.294, whole genome shotgun sequence.
ACCESSION   DAKISA010000288 DAKISA010000000
VERSION     DAKISA010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.294"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            245..490
                     /locus_tag="OVW45_004584"
     CDS             245..490
                     /locus_tag="OVW45_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528835.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMVGIRQIRLIT"
     gene            complement(507..1139)
                     /locus_tag="OVW45_004585"
     CDS             complement(507..1139)
                     /locus_tag="OVW45_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HCV5528836.1"
                     /translation="MDPLHAVYLTVGLFVITFFNPGANLFVVVQTSLASGRRAGVLTG
                     LGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAWCSMRRQSTPQMST
                     LQQPISASWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTWARLMAWAGIVLAS
                     VIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYEGVTQR"
BASE COUNT          350 a          277 c          275 g          256 t
ORIGIN      
        1 tcgcagccat gcggtgtata aaaaatgatc tcatgcagat gttttgtgaa tgtgtgggtt
       61 gacattcata tgaaaaaaat cataattcca tcatgtttgt gtggggtctt ttctgtacct
      121 tacgcatcgc actcaagcct gacagaaaat atgctgtaag gctcatatca aaaaccgcca
      181 ttagctcatc aggaagagca gacgacaacc aggattgttg tatggtacgg ggttcgaggc
      241 ctcgatggcg gtccaacttt tcaaaactct tctgaatcag atccccttat tatcatccct
      301 gcaaagtgga acattgccat tgtttggcta ttcaggacgg gtcagaccaa tgaaaaaagc
      361 ccacacaggg gagagtgggc tgaaatggga agctaaagac tcaagtaaac ttatcggaaa
      421 taaggaccac gcattacggg ggctatcatc gccgatggtc gggattcggc aaatcaggtt
      481 gattacgtag agagtaaatt atctgctcac cgctgcgtca ccccttcgta aatcaggcgt
      541 agcgcgaata caccaataat tgcaccaata acccgactgg caacgcgttg catacgccca
      601 taagcacgac gcacagcggg caaagaaaac gcctgactaa gaaagactcg ccagataact
      661 gatgcgagca caatccccgc ccaggccatt aaacgtgccc atgttggtgt ttcggcattt
      721 aatgttactg agaaaatact gataaaaaat aaaacggttt gcgggttaga gagatcggta
      781 attaatccgc ggcgaaaaaa gacataccag gaggcgctaa tcggttgttg tagtgtgctc
      841 atttgcggtg ttgactggcg gcgcatgctg caccacgcaa accataagag ataagcgccg
      901 ccgacgattc tgataagcga aaaaatctcc tcacactgcg ttattagcgt tgcaagacca
      961 aacaaaccca acccggaata aaatgcatcg cccagcgcca cgcccagtcc ggtcagcacc
     1021 cctgcgcgtc gaccggaagc caggctggtt tgtactacca caaagagatt ggctcccgga
     1081 ttaaaaaaag taatcacgaa cagtcctacg gtcaggtaaa cggcgtgcaa aggatccata
     1141 gtgatttcat ccataaat
//
LOCUS       DAKISA010000289         1157 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.295, whole genome shotgun sequence.
ACCESSION   DAKISA010000289 DAKISA010000000
VERSION     DAKISA010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.295"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            6..407
                     /locus_tag="OVW45_004586"
                     /pseudo
     CDS             6..407
                     /locus_tag="OVW45_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            572..>1157
                     /locus_tag="OVW45_004587"
     CDS             572..>1157
                     /locus_tag="OVW45_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCV5528837.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRV"
BASE COUNT          322 a          260 c          276 g          299 t
ORIGIN      
        1 ggtgacgagt ctcatcgtct atggaaacga cattcgtgct ggatgcactg gagcaggcgt
       61 tgtgggcccg tcgcccgtct ggcaccatcc atcacagcga taaaggctct cagtatgtgt
      121 cactggccta taaggcgcga ctaaaagaag caaaactgct ggcatcaaca gggagtactg
      181 gtgactcgta tgacaacgcg atggcagaga tcatcaaggg cctttacaaa gcggaggtaa
      241 tacaccgtaa gagctggaaa aaccggacag aagtggagct ggccacactc acgtgggtgg
      301 actggtataa caatcgacga ttgctggaaa ggctgggcca tatcccaccg gcagaagcag
      361 aaaaagctta ttatgcttcc atcggaaatg atgatctggc agcctgagtt cacagataaa
      421 acactctcca ggaaacccgg ggcggttcag ttcacaaaaa cacattaatg cagtaactat
      481 attttccttt ctggtgataa cgtcgggtta tcattagctt cttcagctat tttactttta
      541 tatcccttgt aaacatcatg gagaatccat agtgaataaa gtttattctc ttaaatattg
      601 ccccgtcacc ggggggctta ttgctgtctc tgaacttgcc cgcagggtaa taaaaaagac
      661 atgccgaaga ttaacgcata ttcttctggc tggcattcca gcaatctgtc tgtgttactc
      721 tcagatatcc caggcgggta ttgtccgttc cgatattgcc tatcagattt atcgtgattt
      781 cgccgaaaac aaagggcttt ttgtacctgg tgccaatgat attccggtat atgataagga
      841 cggaaaactt gtgggaagac tgggtaaagc cccaatggcc gatttcagca gtgtgagctc
      901 aaatggcgtt gctacgcttg tatcgcctca gtatatcgtc agcgtaaagc ataacggagg
      961 atatcggagt gtgagctttg gtaatgggaa aaatacatat tcccttgttg accgtaataa
     1021 ccacccttct attgacttcc atgctccacg tctgaataaa ctggttacag aagttattcc
     1081 ctcagcggta acatcagaag gaaccaaagc caatgcttat aaatacactg aacgttacac
     1141 cgctttttat cgggtgg
//
LOCUS       DAKISA010000290         1149 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.296, whole genome shotgun sequence.
ACCESSION   DAKISA010000290 DAKISA010000000
VERSION     DAKISA010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.296"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..265)
                     /locus_tag="OVW45_004588"
     CDS             complement(<1..265)
                     /locus_tag="OVW45_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCV5528838.1"
                     /translation="MENSRISGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSV"
     gene            complement(281..>1149)
                     /locus_tag="OVW45_004589"
     CDS             complement(281..>1149)
                     /locus_tag="OVW45_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCV5528839.1"
                     /translation="ALLILATLINRFLLWRLPERKGGEVTLRIRTWWGIVICFSLVIS
                     GPRWMTLAFFALISFLALKEYCMLISVHFPRWLYWVIPLNYLLIGFNCFELFLLFIPL
                     AGFLILATWRVFVGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPSMNIQGGALLV
                     LFLLALTASNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMIASMIIGPLLT
                     PLNTLQAILAGLLIGISGFCGDVVMSAIKRDVGVKDSGKLLPGHGGILDRIDSLIFTA
                     PVFFYFIRYCCY"
BASE COUNT          372 a          293 c          228 g          256 t
ORIGIN      
        1 gcactgaacg cgcaagagat gggctataac cacgcggccc cgaagttttt ccatgccccc
       61 gggcatccca tgcataaaaa gcagtatctg gcatcgccag ttcatcaacg atatgttgta
      121 gacgaccaga atgttcatgg ccacgatgaa ataagacgat gacctttttc gcccctggct
      181 gtaaagcggg ccagtgccga taaaacaacg ctgtattatc actggtggta aaaaaatgtt
      241 ccccagagat gcgtgaattt tccattttct ctattttcct tcagtaacag cagtaacgaa
      301 taaaataaaa aaataccgga gcggtgaaaa ttaatgagtc aatcctgtca aggattccgc
      361 catgtccagg taatagtttc ccgctatctt taacaccaac atctcgtttg atggctgaca
      421 tcactacatc gccgcaaaaa ccactaatac caattaacaa acccgctaat attgcctgta
      481 atgtatttag cggtgtcagt aacggtccaa tgatcatcga cgctatcatg gtggtgatta
      541 cgccgccgac cagaccttcc agcgtttttc ccgggctgac tttggggacc acttttcttc
      601 tgccgcagga tttcccccat aaatactgtg caatatcgtt tgacgcggtt aatgccaaaa
      661 gaaataacac cagtaaggcc ccgccctgga tattcatgga tggcaacatt aataaccagg
      721 cggcatgact caaggcgaaa acggtcatta tccagcccca aaaaatggcg ctcacggtat
      781 gcagaaaacc ggaggggtca cccacaaaaa ctcgccaggt ggctaatatc agaaacccag
      841 ccaaaggtat aaataacaga aacagctcaa agcaattaaa accgataagc aaatagttaa
      901 gaggaataac ccaatataac caccgcggaa aatgtacgga tataagcata caatattctt
      961 tcaatgccag aaaacttatc agcgcaaaaa acgccaacgt catccagcgt ggacctgaaa
     1021 tcaccagtga aaaacaaatg acgatgcccc accaggtacg aatacgtaat gtaacctcac
     1081 ccccttttct ctccggtaac cgccatagca gaaaacggtt tatcagcgtt gctaaaatta
     1141 acaacgcga
//
LOCUS       DAKISA010000291         1145 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.297, whole genome shotgun sequence.
ACCESSION   DAKISA010000291 DAKISA010000000
VERSION     DAKISA010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.297"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..187
                     /locus_tag="OVW45_004590"
     CDS             <1..187
                     /locus_tag="OVW45_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCV5528840.1"
                     /translation="REYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNN
                     YRIALRKYQHYQRQTCT"
     gene            320..1120
                     /gene="tcyJ"
                     /locus_tag="OVW45_004591"
     CDS             320..1120
                     /gene="tcyJ"
                     /locus_tag="OVW45_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCV5528841.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
BASE COUNT          318 a          249 c          306 g          272 t
ORIGIN      
        1 gcgtgaatat gtcgttcgtc tgcgccgttt gggtaatcat ctacacgagc aaaatatttc
       61 cctcgatcag ctgcaggacg gtttccttga tgaaatcctc gccccgtggc tgcccaccac
      121 cagcaccaac aattaccgca ttgcgttacg gaagtatcaa cactatcagc gtcaaacgtg
      181 tacctgactt gtgcagaaat cctccagtct gcctgcttct gatatatatt aaataagaat
      241 aagatgtagc ggagttgttt ttgtgtttac aaacaatggc tctacactgc aaacagacat
      301 aacaacattc ggggtgaata tgaaattagc acatctggga cgtcaggcat tgatgggtgt
      361 gatggccgtg gcgctggttg cgggtatgag cgttaaaagt tttgcagatg aaggtctgct
      421 taataaagtc aaagagcgcg gcacgctgct ggtagggctg gaaggaactt atccgccgtt
      481 cagttttcag ggggatgacg gcaaattaac cggttttgaa gtggaatttg cccaacagct
      541 ggcaaaacat cttggcgttg aggcgtcact aaaaccgacc aaatgggacg gtatgctggc
      601 gtcgctggac tctaaacgca ttgatgtggt gattaaccag gtcaccattt ctgacgagcg
      661 caagaaaaaa tacgatttct ctacccctta caccatttcc ggtattcagg cgctggtgaa
      721 aaaaggtaac gaaggcacca ttaaaacagc cgatgatctg aaaggcaaaa aagtgggtgt
      781 cggtctgggc accaactatg aagagtggct gcggcagaat gttcagggtg tcgatgtgcg
      841 tacctatgat gatgacccga ccaaatatca ggatctgcgc gtaggccgta tcgatgcgat
      901 cctcgttgac cgtctggcgg cgctggatct ggtgaagaaa accaacgata cgctggcagt
      961 aaccggtgaa gcattttccc gtcaggagtc tggcgtggcg ctgcgtaaag gaaatgaaga
     1021 tctgctgaaa gcagtgaatg atgcaattgc ggaaatgcaa aaagatggca ctctgcaagc
     1081 cctttccgaa aaatggtttg gtgctgatgt gaccaaataa tcagcataat gacaaaaaag
     1141 ggcgc
//
LOCUS       DAKISA010000292         1119 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.298, whole genome shotgun sequence.
ACCESSION   DAKISA010000292 DAKISA010000000
VERSION     DAKISA010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.298"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..1000
                     /gene="htrE"
                     /locus_tag="OVW45_004592"
     CDS             <1..1000
                     /gene="htrE"
                     /locus_tag="OVW45_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HCV5528842.1"
                     /translation="WASGQNRSNYSIGYSNSASWGSYSVSAQRSWNEDGDTDDSVYLS
                     FTIPIEKLLGTEQRTSGFQSIDTQMSSDFKSNNQLNVSSSGYSDNARVSYSVNTGYTM
                     NKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFVLHSGGLTFSNDS
                     FSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRIALDINDLENDVE
                     LKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADIYDEQGNVIGNVG
                     QGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSIILNGIRCQIQ"
     gene            1017..>1119
                     /locus_tag="OVW45_004593"
     CDS             1017..>1119
                     /locus_tag="OVW45_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCV5528843.1"
                     /translation="MIKITPHKIAILMGILLSPSVFATDINVEFTATV"
BASE COUNT          337 a          221 c          265 g          296 t
ORIGIN      
        1 ctgggcttcc ggacaaaatc gtagcaatta ctctattggc tacagtaaca gtgcatcctg
       61 gggcagctac agtgtcagtg cccagcgttc atggaatgaa gacggcgaca ctgacgatag
      121 cgtttatctc agtttcacca ttcctattga aaaattactt ggcactgaac aacggacttc
      181 aggtttccag agtattgata ctcaaatgag cagtgacttt aagagtaata accaactcaa
      241 cgtcagtagc agtggctata gtgataacgc tcgcgtcagt tatagcgtaa atactggcta
      301 tacgatgaat aaagccagca aagatttgag ttatgttggg ggttatgcca gctatgaatc
      361 accgtgggga accctggcag gttcagtttc tgcaaatagc gataacagtc gtcaagtgtc
      421 tctcagcacc gacggtggtt ttgtattgca tagcggcggc ctgaccttca gtaatgacag
      481 ttttagcgac tccgatacac tggcggtagt tcaggctcca ggtgctcaag gagcgcgaat
      541 aaattatggc aacagtacta tcgatcgatg gggttatggt gtcaccagcg ctctttctcc
      601 ttatcatgaa aaccgtatcg cgctggatat caacgatctt gagaacgatg ttgaattaaa
      661 aagtaccagt gcagtagctg taccgcgtca gggttcagtc gtctttgctg attttgaaac
      721 cgtgcaaggg caatcagcca ttatgaacat cacacgaagt gatggtaaaa atattccatt
      781 tgctgcagat atttatgatg agcaaggcaa tgtcattggt aatgttggac agggtggaca
      841 agcatttgtt cgtggcattg agcagcaggg aaatatcagc attaaatggc tcgaagaaag
      901 taaacccgta agttgtcttg cgcattatca acaaagttca gaagcagaaa aaatagcgca
      961 atctattatt ctgaatggaa ttcggtgtca gattcagtaa ctacaaggaa cgtcaaatga
     1021 taaaaataac gccacataaa atagcgatac tgatgggaat attattatca ccatcagtat
     1081 ttgcaacgga tattaatgta gagtttacag ccactgtca
//
LOCUS       DAKISA010000293         1112 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.299, whole genome shotgun sequence.
ACCESSION   DAKISA010000293 DAKISA010000000
VERSION     DAKISA010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.299"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            763..1059
                     /locus_tag="OVW45_004594"
     CDS             763..1059
                     /locus_tag="OVW45_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCV5528844.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT          332 a          212 c          237 g          331 t
ORIGIN      
        1 aagtttagct gtctcataat ccaaaaacaa ccttttgaga ctacttgagg gtgcattcta
       61 ccgttacgtt gaaagtgtct caaaaggaag tccttttaag actggttatg aaggagctat
      121 gaggttgatt aggggttggt acaagcccaa ctaaccgaat caactatgaa gattttagga
      181 gggtaactgt aaccatcact ttctaacttt cacgtaggaa attgttaagt ggtttgatag
      241 ttacaccatg tgaccaagca tctatatatc gttatatagc cctgtatata gcactatatg
      301 tagtgtcgag agaggaacga ctaacacgat acagtttacc ctgaaaagag acttcacaga
      361 ttctttatat atttcaataa gttagggtaa aaagggacta taccgagact gtatagaatc
      421 tgtgtagaat tgcatagata cttggaaatc tactattaaa ttgcataggg actcacctga
      481 tattgtcctg tttttaacag gtctcctaga aaaccgaata gacttcattc aagggtcatt
      541 gaagtcataa tcatttcagt agaaaaacca agtctcgatt ttaaatgcaa atagcaatca
      601 tattcaataa ggcaagacga tcaggatcag tgaaaatgtc atttttggcg taattgacct
      661 tactttatgc atggtcttaa caaagacatc atctttccgt tgcagatctt cgccctttca
      721 aataacgtac tttacaactt tcccgaacaa ggagttgtgc ccgtgttgta tgttatttac
      781 gctcaagaca aagctgatag cctcgaaaaa cgcctttccg ttcgtccggc acatttagca
      841 cgtttacagt tactgcatga tgaaggtcgt ttgttgacag ctggtccaat gccagcggta
      901 gacagtaacg atccaggggc tgcgggattt actggctcaa ccgttattgc tgaatttgaa
      961 tcgctggaag cagctcaggc ctgggccgat gctgacccgt acgtagcggc aggtgtctat
     1021 gaacacgttt cagtgaaacc atttaagaaa gtgttctgaa tagagattca atatttaagg
     1081 gcacggtttt tgcaaccgtg ttcttgtttg gc
//
LOCUS       DAKISA010000294         1087 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.300, whole genome shotgun sequence.
ACCESSION   DAKISA010000294 DAKISA010000000
VERSION     DAKISA010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.300"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            528..>1087
                     /gene="fimB"
                     /locus_tag="OVW45_004595"
     CDS             528..>1087
                     /gene="fimB"
                     /locus_tag="OVW45_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HCV5528845.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTTSNAGRFYG"
BASE COUNT          333 a          189 c          233 g          332 t
ORIGIN      
        1 ccaggataaa aaggaatgta acaatctcat ggcgtaagct gacgaaccag gcaggaataa
       61 tcgctaggga cctaagcatt agcatgataa tagccactaa gaaattactg tgttccatga
      121 aatagccatt ttgtggcaaa tggagttgac taataatgtc atatgtgaga cggctagttg
      181 aacgaatatt aaattttgct gaatttttta tgttgatttt acttgttaca gaacatatca
      241 catgatatat agataagatt agttgcatta atgatgaggg ttattattag attcgtatca
      301 ggttgataaa tatataaagg tacatagcat gcaagagcat ggcgtttgta tggcaaagtt
      361 attataagta actatcgcta ctccatttaa gttcgctcag aagaactggt ccacttacgt
      421 tagttattaa gcaaacgttc gcttttataa acataatcag gattaaaatg ttggattatt
      481 gctaacccag cacagctagt gcgcgtctgt aattataagg gaaaacgatg aagaataagg
      541 ctgataacaa aaaaaggaac ttccttaccc atagtgaaat cgagtcactc cttaaagcag
      601 caaataccgg gcctcatgca gcacgtaatt attgtctgac tttgctttgt tttattcatg
      661 gtttccgggc gagtgaaatt tgtcgattga ggatttcgga tattgatctt aaggcaaagt
      721 gtatatatat ccatcgatta aaaaaaggct tttcaacaac gcacccgcta ttgaacaaag
      781 aagttcaggc tttaaaaaac tggttgagta tccgtacttc ttatccgcat gctgagagcg
      841 agtgggtatt tttatcacgt aaggggaatc cgctttctcg gcaacagttt taccatatta
      901 tctcgacttc cggtggtaat gccgggttgt cactggagat tcatccgcac atgttacgcc
      961 attcgtgtgg ttttgctttg gcgaatatgg gaatagatac gcgacttatc caggattatc
     1021 ttgggcatcg caatattcgt catactgtct ggtataccac cagcaatgca gggcgttttt
     1081 acggcat
//
LOCUS       DAKISA010000295         1075 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.301, whole genome shotgun sequence.
ACCESSION   DAKISA010000295 DAKISA010000000
VERSION     DAKISA010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.301"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            83..>1075
                     /locus_tag="OVW45_004596"
     CDS             83..>1075
                     /locus_tag="OVW45_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HCV5528846.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLNPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSCVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLK
                     ENPKFRVIYQPVYSPWVNHVERLWQALHDTITRNHQCSSMWQLLKKVRHFMETVS"
BASE COUNT          292 a          274 c          288 g          221 t
ORIGIN      
        1 agatcacttt gagggaactt agcccggatt gtgcgatctg atcaatcgcc aaatcaaaac
       61 aaatcaccaa ccggactgag caatgccgat catagcacca atttcccgtg acgaacgacg
      121 cctgatgcag aaagccatcc ataaaacaca cgataaaaat tatgcccgca gactgactgc
      181 catgctgatg ctgcaccggg gcgaccgtgt cagcgacgtt gccagaacgc tctgctgcgc
      241 ccgttcctct gttggacgct ggattaactg gttcacgcag tcgggtgttg agggactgaa
      301 atcattacct gccgggcgtg cccgtcgctg gccgtttgag catatctgca cactgttacg
      361 tgagctggta aaacattctc ccggcgactt tggctaccag cgttcacgct ggagtacaga
      421 actgctggca ataaaaatca atgagataac cggttgccag ttaaatgccg gaaccgttcg
      481 ccgctggttg ccgtctgcgg ggattgtgtg gcgaagggct gcgccaactc tgcgtatccg
      541 tgacccgcat aaagatgaaa agatggcagc aatccataaa gcactggacg aatgcagcgc
      601 agagcatccg gtcttttatg aagatgaagt ggatatccat cttaatccca aaatcggtgc
      661 ggactggcaa ctgcgcggac agcaaaaacg ggtggtcacg ccgggacaga atgaaaaata
      721 ttatctggcc ggagcgctgc acagcgggac aggtaaagtc agctgtgtgg gcggcaacag
      781 caaaagttcg gcgctgttca tcagcctgct gaagcggctt aaagcgacat accgtcgggc
      841 gaaaaccatc acgctgatcg tggacaacta cattatccac aaaagccggg aaacacagag
      901 ctggctgaag gagaacccga agttcagggt catttatcag ccggtttact cgccatgggt
      961 gaatcatgtt gaacggctat ggcaggcact tcacgacaca ataacgcgta atcatcagtg
     1021 cagctcaatg tggcaactgt tgaaaaaagt tcgccatttt atggaaaccg tcagc
//
LOCUS       DAKISA010000296         1049 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.302, whole genome shotgun sequence.
ACCESSION   DAKISA010000296 DAKISA010000000
VERSION     DAKISA010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.302"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..120
                     /locus_tag="OVW45_004597"
     CDS             <1..120
                     /locus_tag="OVW45_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCV5528847.1"
                     /translation="EFQQVYKSVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
     gene            120..410
                     /locus_tag="OVW45_004598"
     CDS             120..410
                     /locus_tag="OVW45_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCV5528848.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHDPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            407..961
                     /locus_tag="OVW45_004599"
     CDS             407..961
                     /locus_tag="OVW45_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCV5528849.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLRDMINGKQKSWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPKWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          299 a          201 c          298 g          251 t
ORIGIN      
        1 gagtttcagc aggtttataa atctgttctg aatgtgctgt ggaactggat tctgttccgt
       61 aaattttcct ctccggaaca agtcgaaaat gtggccgcgc agctgctgga gtttgcgtaa
      121 tggtggattt acgtaaagcg gcgcgggggc agatgtgcac cgtcagaatt cctggctact
      181 gcaatcacga tccggaaacg tctgtgctgg cgcattaccg actggcggga acgtgcggaa
      241 cagcgataaa gccacacgat atgcaggcag cgattgcctg tagctcgtgc cacgatttaa
      301 tcgacgggcg ggtaaaaacc agcgattaca ccaaagaaga attacgcctg atgcatgcag
      361 aaggtgtttt tcgcacacaa gaaatctgga gaaaggaggg atatttatga tttacccaac
      421 gaatacagga aaaagcggag aacaccttcg tctcaccacg ctggaaagtg tctggattca
      481 gggaaaacta cgtatgtggg ggcgctggtc gtatattggt ggcggtaaga cggggaatat
      541 gtttaacctg atgttgacct ctaaaaagct gacaaaaacg gcaattaacg aggcgctccg
      601 gaggatgaaa aaagcaggtc tgaacaagtc tgaacttgag gcttttttgc gggatatgat
      661 taacggtaag caaaagagct ggctggcgca ttgtactgat gcagaggcgt tatgtattga
      721 tcgggtcata agtgaggtgc tggcagagca tccaggattg attagcgtcc ttcggcaacg
      781 gtatgagggg cgggggatga ccaaacgcaa aatggctgaa ctgctgaatg atgcacaccc
      841 gaaatggagt ttaagaacct gtgaaagacg cattgagcat tggctaaagg tggcagaatt
      901 tattttgtac aaaccaatgg ttatggcttt tggtatagag aaaaaagtta ttgctttttg
      961 acgtaaaaac tgcttcaatt cttgtacgct tcgcaaagct gtaccgcgag gcgaatagca
     1021 gacatggaca tttgaaagag cccgcttta
//
LOCUS       DAKISA010000297         1047 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.303, whole genome shotgun sequence.
ACCESSION   DAKISA010000297 DAKISA010000000
VERSION     DAKISA010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.303"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            285..389
                     /locus_tag="OVW45_004600"
                     /pseudo
     CDS             285..389
                     /locus_tag="OVW45_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            729..986
                     /locus_tag="OVW45_004601"
     CDS             729..986
                     /locus_tag="OVW45_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HCV5528850.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT          357 a          180 c          198 g          312 t
ORIGIN      
        1 tatcatacag ataattactg gttaacccta tatagggcat tttaaagtgt tgtgatctta
       61 cccagcaata gtggacacgc ggctaagtga gtaaactctc agtcagaggt gactcacaag
      121 acaaaacagt ttcaaccagt aaaaaaacgt aaacaccatt cgcatgaatt tcgtagttaa
      181 gatctgaagc ttgcagaatg catcggtgtt actgccggag tccgtgaact tagtctgcat
      241 gaatcaccac agctttacat ctgtcgcagt aaacagcaaa atcagcaaac ttacttaatt
      301 atatcgagtg cgattacaat cgctggcgat ggtacagttg gtgtggcggt ctcagtccgg
      361 aacaatttga aaatcagaat ctcacttaaa gcggcatcca ttttacaaaa gatggccgtc
      421 tgtgtatttg taatgaatac cttctacatg tttagagtgc ataatccacg acaaggcgat
      481 atcctaaaaa ttagctagca ggcaactttg atggcataga taatgttttt agtaaaaaag
      541 agttgactga atataataca gcctcttcct acataagaag gaatgtgctc acaaaaaaaa
      601 accattagtc tgcttcctgt tcttacaata atcagtatta tgtattttat ttatatagca
      661 aaatatgctt gttacacctg tcataattag gcatggtaaa attattgact gaaccctggg
      721 ggaggaagat gcacagtgat tattataata tggtttttgg tgaaaaattg gccaatattt
      781 tatatgaggc taactctcaa tttttttacg agagaaatgt catagaagag gcagtcaacg
      841 ctttattctg tgagcgtgaa ataataaaca acaagaacat aataaagaag ctgatgttct
      901 ttttgtcaga tgttaatcac accaagaaag atgttgttca gagcgcactt aatattatca
      961 ttgacattac atcaggtgat atttgagatt ttacataaaa gataatctct caaggagtct
     1021 tatctgaaaa cgaaatatat agtccct
//
LOCUS       DAKISA010000298         1023 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.304, whole genome shotgun sequence.
ACCESSION   DAKISA010000298 DAKISA010000000
VERSION     DAKISA010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.304"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(77..865)
                     /locus_tag="OVW45_004602"
     CDS             complement(77..865)
                     /locus_tag="OVW45_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCV5528851.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(905..>1023)
                     /locus_tag="OVW45_004603"
     CDS             complement(905..>1023)
                     /locus_tag="OVW45_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCV5528852.1"
                     /translation="KEAIRFASAVAAMKCTQPGGRAGIPNREQTESFLSLYA"
BASE COUNT          255 a          263 c          239 g          266 t
ORIGIN      
        1 atcgctggca ggtttttttt gcttatggaa aacaaaatcc gctgaaattc ccagcggatt
       61 ttccgaaatt tcaatttcag gatacttctt gctcggcaac caccagttca acattctctt
      121 cacttaacaa ctggcagaaa gaattgggag gcggggcatc agtaaaaaaa gccctgacat
      181 tccgtgcatt gccaattgaa acggcagcag acgcggcaaa cttcgtatga tcggccacta
      241 acaatgtatt ccgcgcatgt ttaatcatcg ttctcgcgac taacgcttca tttacatcaa
      301 attccagtag ggtgccgtca tgttcaatgg cacctatact ggtgattaaa taatctgcgc
      361 ggaagccttc aataaaatcc acggctcctg ggccgataat cccgccgtta tgagcgcgta
      421 aagtgcctcc cggcaccatc acttcaatat cctgattctt ataaagaatc tgtgccacac
      481 gtaggctgtt agtgataatg cgtaaatcac gccggttgag taatgccctg gcaacggctt
      541 ctacggttgt accgatggtg ataaagacgg tacagcgttc gggaaggtaa tcggcgactg
      601 cctcagcgat cgcccgtttc tcggcggtga gcgaaagttc ccgttgctca aaagcggtat
      661 tcatgacgct ggagacgcgc ccggcccctc catgatgacg ggtgatcaag ccctgctcac
      721 tgagttttcg gatatcccgg cgcacggttt gcgttgaaac atccagcagt tgtgccagtt
      781 cttcaatatt catataaccg cgttctgcga ttagatgaac gagttggtca tggcgtggat
      841 tacctgtcac ttcgatgata ctcatgaata ctcccttgcc cctcctcggt agatgtttac
      901 cattttacgc ataaagtgac aaaaatgatt cggtttgctc acgattagga atgccggccc
      961 gaccgccagg ctgggtacat ttcatcgcgg caaccgcact ggcaaaacga atagcctctt
     1021 ttg
//
LOCUS       DAKISA010000299         1005 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.305, whole genome shotgun sequence.
ACCESSION   DAKISA010000299 DAKISA010000000
VERSION     DAKISA010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.305"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..731)
                     /gene="rutD"
                     /locus_tag="OVW45_004604"
     CDS             complement(<1..731)
                     /gene="rutD"
                     /locus_tag="OVW45_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCV5528853.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDP"
     gene            complement(739..>1005)
                     /gene="rutC"
                     /locus_tag="OVW45_004605"
     CDS             complement(739..>1005)
                     /gene="rutC"
                     /locus_tag="OVW45_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCV5528854.1"
                     /translation="NVLFADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWK
                     NYAAINEIYAEFFPGDKPARFCIQCGLVKPDALVEIATIAHIAK"
BASE COUNT          214 a          296 c          282 g          213 t
ORIGIN      
        1 gggtcggtca cgttgcaggc gtgtccgcca tagcgcatca ccattttctg gctatcgggc
       61 agggcggcat gaagctcact ggaacatgct gatggcacca gcagatcatc actggcgcag
      121 atgatttgca ccgggcagcg gatgcgatcc gcatggtgac taaagtcagc gcgtttgagg
      181 gcgttaagtc gacgcagtaa attatttttg ccctgaaaat gcgccagtgc cagcgcgtct
      241 tctgcctcaa ggcgaggtgc gcgggccgcc atccagtcgg cgggatagag gaacaacggc
      301 tgcgcttcca cccatgcctg cgcgccgccg ctatacagta atcgttcgcg aacctgaaaa
      361 cagcggcgcg tatgggcgtt tattcgtagc cagccgttaa cgctgatcag cacagttacc
      421 gacgcgggat aatccagcgc cagctgcatt cccaccagcg caccgagcgc atggccgacc
      481 accgcgtaat gctcaatccc tgcggctacc agcgcctgat gcagttccgc tgccatctgg
      541 gcgatactgt aatcttctgc cagcgtgtcg ggattattgc cggtgccgcg ctggtcgtaa
      601 cagactacct gatactcctg ctccagcacc gccagttgcg gtaaccagta actgccgcta
      661 ccgccaagac ccgaaatcaa caccactacg ggcgcatcag cataaggggg aggtgagagt
      721 gaaagtttca tcgcggcctc acttggcgat atgcgcaatt gtggcgattt ccaccagcgc
      781 gtcaggtttt accagtccgc actgaatgca gaatcgcgcc ggtttatcac ccggaaaaaa
      841 ctcggcgtag atttcgttaa tcgcggcgta atttttccag tcggtaataa agatgctgtt
      901 gaaggtcaca tccgccatcg tgccacccgc cgtctcgatc accttgcgga tagtttccag
      961 aacgtggcgg gtttgcgcct ttgggtcatc ggcaaacagc acgtt
//
LOCUS       DAKISA010000300         1002 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.306, whole genome shotgun sequence.
ACCESSION   DAKISA010000300 DAKISA010000000
VERSION     DAKISA010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.306"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..>1002)
                     /locus_tag="OVW45_004606"
     CDS             complement(<1..>1002)
                     /locus_tag="OVW45_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HCV5528855.1"
                     /translation="PTTIAVQESNYPLKFNQQNSQTAALLPRYDLPAPMLDRPAKGAD
                     GALLALTAGKNRETIAAQFAQGGANGLAGYPTTSNMWVIGKSKAQDAKAIMVNGPQFG
                     WYAPAYTYGIGLHGAGYDVTGNTPFAYPGLVFGHNGVISWGSTAGFGDDVDIFAERLS
                     AEKPGYYLHNGKWVKMLSREETITVKNGQAETFTVWRTVHGNILQTDQTTQTAYAKSR
                     AWDGKEVASLLAWTHQMKAKNWQEWTQQAAKQALTINWYYADVNGNIGYVHTGAYPDR
                     QSGHDPRLPVPGTGKWDWKGLLPFEMNPKVYNPLSGYIANWNNSPQKDYPASDLFAFL
                     "
BASE COUNT          243 a          265 c          226 g          268 t
ORIGIN      
        1 caaaaaggca aacagatccg aagcgggata atctttttgg ggagaattgt tccagttagc
       61 aatatatccc gacagggggt tatacacctt agggttcatt tcaaaaggca atagcccttt
      121 ccagtcccat tttcccgtac caggaacggg taatcgcgga tcatggcctg attgacgatc
      181 tggataagca ccagtatgaa cataaccaat attgccgttt acatcagcat agtaccagtt
      241 gatggtcagt gcttgtttcg ctgcctgctg tgtccactcc tgccaatttt tggccttcat
      301 ctgatgagtc caggccagca aagacgccac ctctttacca tcccatgcgc gggatttagc
      361 gtaagccgtt tgtgtcgtct ggtcagtttg gagaatgttg ccatgcaccg tacgccagac
      421 agtaaaggtc tctgcctgac catttttcac cgtaatggtt tcctcacggc ttaacatttt
      481 cacccactta ccattatgca agtagtagcc tggtttctct gccgacagcc gttcagcaaa
      541 aatatcgaca tcatcgccga aacctgccgt tgatccccag gaaatcacac cattatgacc
      601 aaaaaccagc ccaggatagg caaatggtgt attgccagtg acatcataac cagcaccgtg
      661 cagaccaata ccataagtat acgcaggcgc ataccagcca aactgcggac catttaccat
      721 gattgctttc gcatcctggg ctttgctttt gccgatcacc cacatattgc tggtcgttgg
      781 ataccccgcc agaccattgg caccaccctg tgcaaattgt gcagcaatag tttcccggtt
      841 cttccctgct gttaacgcca gcagtgcgcc atccgcccct tttgctggtc ggtcaagcat
      901 tggtgcaggt aaatcgtagc gtggcaacag agctgctgtt tgcgagtttt gctgattaaa
      961 tttaagtggg tagttactct cttgtacggc aatagtggtt gg
//
LOCUS       DAKISA010000301          982 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.307, whole genome shotgun sequence.
ACCESSION   DAKISA010000301 DAKISA010000000
VERSION     DAKISA010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.307"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            381..>982
                     /locus_tag="OVW45_004607"
     CDS             381..>982
                     /locus_tag="OVW45_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCV5528856.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT          261 a          188 c          242 g          291 t
ORIGIN      
        1 agctttatga gaaaggtgaa tccccttaaa cagagcgtat ttttcagtga ttccctttaa
       61 agtgaataga ggacgtattg gctatttgtg cttatttttg tctcattgcc atttgttatt
      121 ttggcttgta gtcgcggaat ttcattttta attgcatgcg tactaaccac caatataagg
      181 aaatcctcaa aattgtttta aatagtgccc tcgacatgta atttcaggcg tttagctgta
      241 actatttata tcggtaacta ttttctacgc gtcatctctg tagaatggcc accgccaaat
      301 ggcgaattct tattttttct cgcgggtaat acccttccac tacttaagtc tttttaacgt
      361 attcaggtgg agattcagtt atgcaaatag tcatgtttga caggcagtca atatttattc
      421 atggaatgaa aatcagttta cagcagcgta ttccaggagt gagtattcag ggggccagtc
      481 aggcagacga gttatggcaa aagctggaaa gttaccctga agccttagtt atgctcgatg
      541 gcgatcagga tggtgagttt tgctactggt tgctgcaaaa aaccgtggtg cagtttcctg
      601 aggttaaggt gttaatcacg gcgacggatt gcaataaacg gtggttacag gaagttattc
      661 attttaatgt gctggccatt gtgcctcgtg attcaaccgt cgagacattt gcgttggcgg
      721 taaatagtgc ggcgatgggg atgatgtttc tgcctggaga ctggcggacg acgccggaaa
      781 aggacattaa agatctcaaa tcgttaagcg cccgccagcg ggagatttta accatgttag
      841 cggcgggcga atcaaataag gagatcggca gagcgctgaa tatcagtacc ggaacggtaa
      901 aagcgcatct ggaatccctg tatcgccgtc tggaagtgaa aaatcgcacc caggcggcaa
      961 tgatgttaaa tatctcctcc tg
//
LOCUS       DAKISA010000302          974 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.308, whole genome shotgun sequence.
ACCESSION   DAKISA010000302 DAKISA010000000
VERSION     DAKISA010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.308"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..410
                     /locus_tag="OVW45_004608"
     CDS             <1..410
                     /locus_tag="OVW45_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCV5528857.1"
                     /translation="VTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAKKMMPEYEGGF
                     WHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLWLYHDSG
                     DAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            457..876
                     /locus_tag="OVW45_004609"
     CDS             457..876
                     /locus_tag="OVW45_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HCV5528858.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIARDWSLTD
                     DELETVMQRLDDAFEYGADVSIVHDVVRGLMEEKRASRQVTVPAVMLGKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGENG"
BASE COUNT          222 a          228 c          314 g          210 t
ORIGIN      
        1 ctgtcactct gaatgttgaa gagtgcgacc gtctctcctt tctgccttac ctgtttggca
       61 acgattttct gtatgccgag gcgtatgtgt acgcgctggc gaaaaaaatg atgccggaat
      121 atgaaggcgg cttctggcac ttcatccgcc tgccggacgg tggcggttac atgatgccgg
      181 atggcgaccg tttccacatg gtgaacggtg caaactggtt tgaccgtacc gtgagtgctg
      241 atgccgcagg catcattctg acctcccttg tgattaaccg ccagttgtgg ctgtaccacg
      301 acagcgggga tgcgggactg acacacctgt accggatgcg tgatgcgcag ttgtggagcc
      361 acatcgaatt tcaccccgaa tgtaacgcga tttacgcggc actggactga ttaacggacg
      421 gggcggcaat gccgcccctg taaaggagac gagaacatgt actgtactgt taaagaaatt
      481 atccgcgaag tgctggatac agacgtgccg gacagtgaat gcgtttttgc cgtggtgctg
      541 acccgtgggg atgtgcgtca catagcccgg gactggagtc tgacagacga tgagctggaa
      601 accgtcatgc agcggctgga cgatgccttt gaatatggtg cggatgtcag cattgttcat
      661 gacgttgttc gtggactgat ggaggaaaag cgcgccagcc gtcaggtgac agttccggcg
      721 gtgatgctgg gaaaagtgat ggcgctggca ggcagtgaaa tgaagcgcct gtatgccgtc
      781 gggagtgaga acgggggcga cggtgacgca ttcgtcaggg aagaacgcga agcaatggac
      841 gttgtgttac aggcgctgga cggggagaac gggtaatgaa tatcagcaca gaaacccgcg
      901 aaattctgcg taattacaga gccgtgatta atgcgcggcg tcgtgaaatg gggcagaaac
      961 cgctcaccac cgcg
//
LOCUS       DAKISA010000303          955 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.309, whole genome shotgun sequence.
ACCESSION   DAKISA010000303 DAKISA010000000
VERSION     DAKISA010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.309"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(27..899)
                     /locus_tag="OVW45_004610"
     CDS             complement(27..899)
                     /locus_tag="OVW45_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HCV5528859.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAQGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLCVLQKRASGKSYHSDNSGSGGGSSGGGFSG
                     GGGSSGGGGASGRW"
BASE COUNT          297 a          189 c          197 g          272 t
ORIGIN      
        1 ccgggctggc attttttatg gttgagttac cagcggccgg atgcgccgcc acctccagaa
       61 gaaccgcccc cgccgctaaa tccgccgcca cttgagcctc caccggaacc gctgttgtct
      121 gaatgataac ttttacctga tgctcttttc tgaagtacac ataaacaggt aaagaccata
      181 aatatggtga aagtaaaaaa gaaggaaaat acagcaatgg aaaaaatttt atcggagaac
      241 aggaataaat ctaaaagata gattgctgaa atacagactc cacttttaca tgcgcgacaa
      301 aaattactgc cttgatgaaa aaaccagaaa gggaagaaca tcactgcaaa tacccaaaaa
      361 atagcaaagt aataatgatc actggaagac gctgattcac tttctgaagg attagtcgga
      421 tattggtgtt gtgaagtgag ttggttattc aaagcgttta tagctagctc taatccctga
      481 gctaattttt gttgtttaaa tgcgggtatc atgttgctac ggatgatatc tccagccaga
      541 gcatcggtta ccttttcttc cagcccataa cctacctgga tgcggacagt acgatctgac
      601 caggcaacaa tgatcagtat cccatcatta cgtttggcat ctcctaaacg ccaattgtca
      661 aaaactcttg tagcatattg ttcaattgtc tcgtctttgg ttgtgggaac aattaataca
      721 gcaatttgca cttgggtatt attgaaaata gattctaact tgtgcgtcag ttctttcttt
      781 tcttgtgagt ttaagctact cgtaagatcc gtaacttgtt gctgtaattg tgggacaata
      841 gttggttggg ctgcaacaaa ggaacacacg aaagtgaaca ttagtatgaa atagcgcatt
      901 ataaatttct cattaaatct ggataaataa attgacggcg gcatcggtaa tttta
//
LOCUS       DAKISA010000304          947 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.310, whole genome shotgun sequence.
ACCESSION   DAKISA010000304 DAKISA010000000
VERSION     DAKISA010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.310"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..>947
                     /locus_tag="OVW45_004611"
     CDS             <1..>947
                     /locus_tag="OVW45_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528860.1"
                     /translation="AQQKAKTEKQNQRAQAAAARAQARQQQQIAARKEKAELKLLDVS
                     SSISAMHRLSVAEQYKAIAQAREIAKQYEQGAISLARMNSQMKRLQQQQRKINGNRKA
                     QFAPVKGGSGNSATMGALLFGGATAAAGVMAVSKTSEFMANSFANAETEGELIQRARL
                     GGVDYNLLNNLERWAYDNGIDSLQGDPGRRKILDAIGKDVKERATKSYDEAEYVVDKK
                     TGKGIWKGGDSGINTLMNEGFLTKKDLKDFANNSAGLISKAVNGMVKKGYTDAQIGSR
                     LEDLADDLLLTSKYWQRSAREVQASITQQKAAGQWLTET"
BASE COUNT          324 a          189 c          238 g          196 t
ORIGIN      
        1 ttgctcaaca gaaagctaaa acagaaaagc agaatcaaag agcacaagct gccgcagcaa
       61 gagcacaggc aaggcaacag caacaaatag cagcacgtaa agagaaagca gaattaaaac
      121 tccttgatgt atcaagctct atcagtgcaa tgcaccgtct ctctgtagcg gaacaataca
      181 aagctattgc acaggctaga gagatagcaa aacaatatga gcaaggggct atcagcctgg
      241 cgcgtatgaa tagtcagatg aaacgcctac aacagcaaca acgtaagatt aatggcaaca
      301 gaaaagctca atttgccccc gttaagggag gatcagggaa ttccgccacg atgggcgctc
      361 tgttatttgg aggcgctacg gctgctgctg gcgtgatggc tgttagtaaa acctctgaat
      421 ttatggctaa cagctttgct aatgctgaaa cagaagggga gctaatccaa cgtgcaaggc
      481 ttggtggagt tgattacaac ttacttaata atcttgagcg ttgggcgtat gacaacggga
      541 tcgatagcct ccagggtgat ccgggacgca gaaaaatact agatgccatc ggtaaagatg
      601 taaaggaacg cgctaccaaa agctatgacg aggctgaata tgtagttgat aagaaaacag
      661 ggaaaggcat atggaagggc ggtgattctg gtattaacac tttaatgaat gaaggattct
      721 taactaaaaa ggatttgaaa gactttgcca ataactctgc tggtttaatt tcaaaagctg
      781 tcaacggaat ggtgaaaaag ggctataccg acgcacagat cggtagtcgt ctggaagatt
      841 tagcggacga cctcctctta acttctaaat attggcaaag atcagccaga gaggtgcagg
      901 caagcattac ccagcaaaaa gccgccggac aatggctaac agaaaca
//
LOCUS       DAKISA010000305          943 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.311, whole genome shotgun sequence.
ACCESSION   DAKISA010000305 DAKISA010000000
VERSION     DAKISA010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.311"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            102..>943
                     /locus_tag="OVW45_004612"
     CDS             102..>943
                     /locus_tag="OVW45_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HCV5528861.1"
                     /translation="MHHIKTAADLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IASMDVLRKAVFEGVNIIITHEPTFYSYADLEGDDLEFSWARKIMDYTRGELSYLKII
                     EQKKEFLHKNNLVIIRCHDVMDREPTFGMSKALAQQLELDVTNIVASDEMYCVYAIDP
                     DSAINITKRFAKNLKIYNLPGIQFYGDKARVVRTVGIGAGCFCDPIQYMEYQADYYIT
                     INDSIKTWVQTQYSKDSGLPMAVIGHSVAEEAGMRRLASYLDLHSGYPCIHFTGGCDY
                     DWIE"
BASE COUNT          280 a          145 c          209 g          309 t
ORIGIN      
        1 tttatgggga ggtgataccc actctgagtc tgcatgggca aaacgtttcc cagaaatgtt
       61 gaaattattt tattgctgat gaaacctgtg aaggatgttg aatgcatcat attaaaacag
      121 ccgcggatct taattgtttt ttaaattcaa tggttgctgt ttcgcaacca actgttgata
      181 aaattatttt tggtgctgag gcgacactta ttaataaaat tgggacctgc tggattgcct
      241 caatggatgt tttaagaaag gctgtttttg aaggtgtcaa tatcattatt acacatgagc
      301 ctacatttta ttcttatgct gatctggagg gtgacgatct tgagttttca tgggccagaa
      361 aaataatgga ttatactcgc ggtgaactca gttatttaaa aataattgag caaaaaaagg
      421 agtttttgca taaaaataat ttggtaatca ttcgttgcca tgatgtcatg gatcgagagc
      481 ctacgttcgg aatgtctaag gctttggctc agcagttaga actggatgtt acaaatattg
      541 ttgcttctga tgagatgtac tgtgtatatg ctatagaccc tgacagtgcc attaacatta
      601 ccaaacgttt tgccaaaaat ttgaaaatat ataatcttcc tggcattcaa ttttatggag
      661 ataaagccag agtagtcaga actgttggca ttggggcagg ctgtttttgt gatccaatac
      721 aatatatgga atatcaggct gattattata ttacgattaa cgattcaatt aaaacatggg
      781 tgcagacaca atactccaaa gatagtggct taccgatggc agttattgga catagtgttg
      841 cagaggaagc gggtatgcga cgtcttgcaa gctatttaga tctccattca ggctatccct
      901 gtattcattt tacagggggc tgtgattatg attggattga ata
//
LOCUS       DAKISA010000306          933 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.312, whole genome shotgun sequence.
ACCESSION   DAKISA010000306 DAKISA010000000
VERSION     DAKISA010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.312"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(195..>933)
                     /locus_tag="OVW45_004613"
     CDS             complement(195..>933)
                     /locus_tag="OVW45_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCV5528862.1"
                     /translation="GPTLTFLFVSPLLNPIIVGLMWMTFGWKVTLLYAIIAAGVSVLA
                     SIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKVSCCSPAAKAIENPVVTCC
                     STKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIYGFIPSAWIAAHAGADNPF
                     AIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALIIGSAGASLTEVILLKSMF
                     RMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT          279 a          198 c          199 g          257 t
ORIGIN      
        1 gcttctccga taaaatgtca gagaagcatg attacgtggt tattgtctgt tgatgacttg
       61 ttgaccgagc cgtcatcatt ctcaatgcat tagttaaatc atttgtgatt tttaaggatt
      121 atcctgtcta tgattttcta cttagaagga tatggcacta actatgcatc ttatagttta
      181 ctgctggcat ctatttaaaa tagcaattga gtcaaatagc ccatcaagat agccatacct
      241 aatataaccg tcaggaatgc agctatcatc ggcattctga acattgattt aagtaatatc
      301 acttccgtca ggcttgcgcc ggcactgccg atgattaatg ccattaatgc tcccagaccc
      361 attccttttg tcatcagaac agatgccaga gggataaccg cctcagcccg gatatacagc
      421 ggaataccga caacggcgct taatgggatg gcgaagggat tatctgctcc tgcatgagcg
      481 gcaatccacg ctgagggaat aaagccataa ataaaagatc ctattaatac tcctaacaaa
      541 agatatggca gtacatcttt aaactgttgc aacgcatctt tggtggctag ttttatggga
      601 ttaatactta ctacagcttt ggtactgcaa caggtaacta cggggttctc aatggcttta
      661 gccgctggac tacaacaact cacttttatg ggcgtatatg tcgttcccgg cgaagttttt
      721 gctggagcac aacaatttgc tgatgagctt ttactggcaa tgatatgacg ttcaaacccc
      781 agagaatcca ggataatact ggcaagaacg gagacgccgg ctgcgataat cgcgtacaac
      841 aaggtgactt tccaaccaaa ggtcatccac attagtccga cgataatggg attaagtaat
      901 ggtgaaacaa ataaaaaagt gagagtcgga cca
//
LOCUS       DAKISA010000307          913 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.314, whole genome shotgun sequence.
ACCESSION   DAKISA010000307 DAKISA010000000
VERSION     DAKISA010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..913
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.314"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..552
                     /locus_tag="OVW45_004614"
     CDS             <1..552
                     /locus_tag="OVW45_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCV5528863.1"
                     /translation="IRGWATYHRHVVAKETFNYVDTQIWRAIWRWCVRRHPRKGLRWI
                     AGRYFSFEGRRWIFKAITPEGKTLTLFRAMETPIKRHIKIKGEATPYTPGMEIYFERR
                     LDLIWKGKSKKMKTVVQLWKRQGKHCPQCGQPITNQTGWNIHHRIRKVMGGSDELTNL
                     ELLHPNCHRQLHSREAGAHRKHL"
     gene            complement(667..>913)
                     /locus_tag="OVW45_004615"
     CDS             complement(667..>913)
                     /locus_tag="OVW45_004615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528864.1"
                     /translation="AVTTSYRAAETGDTGAGGGGRLSARAKTPGRLTPAGREAGHAGA
                     SPVTLPVCDVSDSGVFLSYVMELFLMLLLLLFLYLLL"
BASE COUNT          265 a          247 c          245 g          156 t
ORIGIN      
        1 atccggggat gggctaccta tcaccgacac gtcgtggcga aagaaacatt taattatgtc
       61 gacacccaaa tctggcgagc tatatggcga tggtgtgtaa gacggcatcc ccggaaagga
      121 ttgcggtgga tcgccggacg atacttctcc tttgaaggca ggcgctggat ctttaaagcc
      181 ataaccccgg aagggaaaac tctgacgctc ttccgggcaa tggaaacacc gataaagcgc
      241 catataaaaa tcaaaggcga agcaacacca tacacccccg gaatggaaat ctactttgaa
      301 cgtcgtctgg atctaatctg gaaaggcaaa tcaaagaaga tgaagacagt agttcaactc
      361 tggaagaggc aggggaaaca ctgtccgcag tgcggacaac ccataaccaa ccagaccgga
      421 tggaatatac atcaccggat caggaaagtc atgggcggca gtgacgaact gaccaatctc
      481 gagttgttac atccgaactg ccaccgacag ttacacagca gggaagccgg tgctcaccga
      541 aagcacttat aaaggcttga gcggagtgcg gggaaactcg cacgctccgt tcttaggggg
      601 cggcggcgca gcaatgtgcc gctgctaccc gacccggcag acagaacaac actgcagtag
      661 tcagaatcag agcagcagat aaaggaacaa caacaacaac aacatcagga acagttccat
      721 aacataagaa agaaacactc ctgagtcaga cacatcacag acgggaaggg taacgggcga
      781 cgcgccagcg tggcccgctt cccttccggc aggcgtcagc ctgccagggg ttttcgcgcg
      841 ggctgacagg cggcctccac cgcctgcccc tgtatcacct gtttctgctg ccctgtaaga
      901 tgtagtgacc gcc
//
LOCUS       DAKISA010000308          905 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.315, whole genome shotgun sequence.
ACCESSION   DAKISA010000308 DAKISA010000000
VERSION     DAKISA010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.315"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..796
                     /locus_tag="OVW45_004616"
     CDS             <1..796
                     /locus_tag="OVW45_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCV5528865.1"
                     /translation="HDNETELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVAS
                     DVNDPATTCTCALYGFQFQGAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQS
                     KNSQTSSLWDAFAYTLAILYYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITL
                     EQLSKKFRASVSYICHEFTKEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGY
                     ENVDHFAKLFLRHVGCSPSDYRRQFKNCFAEQEILSEFPQPVSLAG"
BASE COUNT          259 a          182 c          197 g          267 t
ORIGIN      
        1 tcatgacaat gaaaccgagt tgatttacgt caagaaaggg gtagcaaggt ttaccatcga
       61 ttcttcgtta tatgtcgcgc acgccgatga cattgtggtg atagaacgtg gcaggctgca
      121 tgcggtggcc tctgacgtta acgatccagc aacgacgtgt acctgtgcgc tgtacggctt
      181 tcagtttcag ggggctgagg aaaatcagct actgcaaccg cattcttgcc cggtaattgc
      241 cgcagggcag ggaaaagaag tcattaaaac cttatttaat gagctaagtg tgattttgcc
      301 gcaaagtaaa aatagccaaa catcttcgtt atgggatgca tttgcctata cattagcaat
      361 tctttactac gaaaacttta aaaatgctta tcgttcggag caggggtata ttaaaaaaga
      421 tgttctgata aaagatattc ttttctatct gaataataat tatcgcgaaa aaatcacttt
      481 agaacagtta tcgaaaaaat ttcgtgccag cgtcagttat atttgccatg aatttaccaa
      541 agagtatcgt atttccccta ttaactatgt tattcaacgg cgtatgacgg aagcaaaatg
      601 gtcactcact aatactgaat tatcacaggc agagatctcc tggcgtgtgg gttatgaaaa
      661 tgtcgatcac tttgccaaac tgtttttgcg ccatgtcggt tgttcgccca gcgattaccg
      721 taggcaattt aaaaattgtt ttgcggaaca agaaatctta tctgaatttc ctcaaccggt
      781 aagtcttgcc ggataaatct ggcggcgcac tttgttaaaa agctgcgccg cctgcagatt
      841 atttgttctg atagtctttc aggatctgcc gacctgctac gtaaatcata atttcgtctg
      901 taccg
//
LOCUS       DAKISA010000309          899 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.316, whole genome shotgun sequence.
ACCESSION   DAKISA010000309 DAKISA010000000
VERSION     DAKISA010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.316"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..568)
                     /locus_tag="OVW45_004617"
     CDS             complement(<1..568)
                     /locus_tag="OVW45_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCV5528866.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKQGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGL"
     gene            complement(568..>899)
                     /locus_tag="OVW45_004618"
     CDS             complement(568..>899)
                     /locus_tag="OVW45_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HCV5528867.1"
                     /translation="ITGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLK
                     QPAQEAS"
BASE COUNT          301 a          212 c          196 g          190 t
ORIGIN      
        1 caagacccat taagatccct ggcaaatatc ccgccataaa tagcgatgct acagaaacgc
       61 cgccagctgt cagggaaaac acaattaaca cgtttgaggg cggtatcagt aatccagtaa
      121 tacaagatga gacgtttaca gcggtagaaa atgccggatc ataaccttgt ttggtctgaa
      181 tgggattcag tgtgccacca acggcagcag ctgccgcaac ggctgaacca gaaatggaac
      241 caaacatcat attcgccagg acgttcacat gaccaagtga accaggaacc cgaccaacca
      301 ttacctgggc gaggttaatc aaacgtatcg caataccgcc gctgttcatc agcgttccgg
      361 caaagatgaa aaaggggatt gccaataggg caaagttatc cagtccatta gccagtcgct
      421 gcgaaacagt gattgccgcg atatcccagg gaaacatcaa aagcattgaa gctacagtgg
      481 caataccgat tgagaacgaa atgggcacac caataaagac cagcacaaaa aaacagccaa
      541 acagtgtaat cgcgatataa ctatccatta actggcctcc tgcgcaggtt gtttcagttg
      601 ttgaatgctt tgattaataa aacacaaggc ataaaacatc atcaccaatc cacttaatgg
      661 cagaatgata tatacgtatc ccatcggtat ttgcatcgcg gcagatacct gtgatgttgc
      721 cagcgtttta tcaattaatt tcaggccacc agtcacaatg accgaaccag caaaacctaa
      781 aataagaacg ttaatgacta tactaagaag taattgcttg cgcttattca gggcgagagc
      841 aaataaatcg atagagagat gtctctgcgc gccgacggta tatgcagcac cggtaatga
//
LOCUS       DAKISA010000310          888 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.318, whole genome shotgun sequence.
ACCESSION   DAKISA010000310 DAKISA010000000
VERSION     DAKISA010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.318"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            221..>888
                     /locus_tag="OVW45_004619"
     CDS             221..>888
                     /locus_tag="OVW45_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCV5528868.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIQ
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDD"
BASE COUNT          234 a          215 c          209 g          230 t
ORIGIN      
        1 ttttttatta attctaaaac ggtaagggta aaaattcagg aattcagaaa aatacaattc
       61 tctgctgcaa gatgaataat gtttatctac agcatttcct taaaagatat gtcaggcttg
      121 cggagtggcg gttaaggaaa tacgacttcc tcctttcaga gtgctccgct tctcactatt
      181 atctcacgca gtattcttaa gggaacgata aggaggaacc atgaacatta ccccgtttcc
      241 gacgctttcg ccggcaacta tagatgccat aaatgttatc ggacagtggc tggcgcagga
      301 tgatttctcc ggtgaggtgc cgtatcaggc cgattgcgtg atccttgcag gcaatgcggt
      361 tatgccgact atcgacgcgg catgtaagat tgctcgcgat cagcaaattc ctttactgat
      421 tagtggtggt atcggtcact cgacaacttt tttgtatagc gccatcgcac agcatccgca
      481 ctacaacact atccgcacca ctggcagagc agaagcgacc atccaggcgg atatcgctca
      541 tcagttctgg cacattccgc atgaaaaaat ctggattgaa gaccagtcaa caaactgcgg
      601 tgaaaacgca cgctttagca tcgcgctatt gaatcaggcc gtagaacgag ttcatacggc
      661 tatcgttgtt caggacccca ccatgcagcg gcgcacgatg gcgacgttcc gccgtatgac
      721 tggggacaat cccgatgcac cacgctggtt aagttatccc ggattcgttc ctcagttagg
      781 aaataacgca gacagtgtaa tctttattaa tcagttacaa ggattatggc cggttgagcg
      841 ttatctctca ctactcactg gcgagctgcc gcgtttacgc gatgatag
//
LOCUS       DAKISA010000311          879 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.319, whole genome shotgun sequence.
ACCESSION   DAKISA010000311 DAKISA010000000
VERSION     DAKISA010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..879
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.319"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..>879)
                     /locus_tag="OVW45_004620"
     CDS             complement(<1..>879)
                     /locus_tag="OVW45_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HCV5528869.1"
                     /translation="ALPISHKTSLLDPLEIRDLHKLVRDTDKVFPNPGNSNLGLMTAF
                     FEAYLDANYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLAR
                     DVATGVLARSMDVDIYNLHPAHAKRIEEIITENKPPFSVFRDKFITMPGGMDYSRAIV
                     VASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEEG
                     KQDDEEKPQPSGAMADEQATAETMEPDATEHHQNTQPLDAQSQVNSVDEKYQELRAEL
                     HEARKNIPPKNPVDAD"
BASE COUNT          188 a          216 c          236 g          239 t
ORIGIN      
        1 tgtctgcatc gacaggattt ttgggcggaa tgtttttccg ggcttcatgg agttctgccc
       61 gcagttcctg atatttctca tcaacagaat ttacctgtga ctgagcatcc agcggctgcg
      121 tgttctgatg atgttcagtt gcatccggtt ccattgtttc agccgttgcc tgttcatctg
      181 ccattgcgcc agatggctgc ggtttttctt catcatcctg ttttccttct tctgttacac
      241 gctgcggcat cggggcagag gagcgaccgc aggcaatatc cacgatttcc ggatcagggt
      301 tggcatgatc ggtttcagtc agtaccttgt tcagatattc agtgacgtgc gcggggatga
      361 cctcgatacc aatcggtgct tctttcactg acgcaaccac gatggcgcgg gaataatcca
      421 tcccgccagg catggtgatg aatttgtcgc ggaaaacaga aaagggcggt ttattttcag
      481 tgataatttc ctcaatgcgt ttagcgtgtg ccggatgaag gttataaata tccacgtcca
      541 ttgaacgggc cagtacgcca gtggctacgt cgcgcgccag cgacgtcaga tcgtggacga
      601 aaccttcgcc gcgatcggtg aggttcccgc cgccagcatt agcaccggaa gccgtgcgag
      661 tgatacgcga aacacgattt cctttcatcc actcttttgt cagcagaccg cgatcggtgt
      721 agttggcatc cagatatgct tcgaaaaaag cagtcatcag tcccagattt gaattgccag
      781 gattagggaa aactttgtca gtatcacgca cgagtttgtg gaggtcgcga atctccagcg
      841 ggtcgagcag actggtttta tgcgaaatag gtaatgctg
//
LOCUS       DAKISA010000312          869 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.320, whole genome shotgun sequence.
ACCESSION   DAKISA010000312 DAKISA010000000
VERSION     DAKISA010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.320"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..552
                     /locus_tag="OVW45_004621"
     CDS             <1..552
                     /locus_tag="OVW45_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCV5528870.1"
                     /translation="IRGWATYHRHVVAKETFNYVDTQIWRAIWRWCVRRHPRKGLRWI
                     AGRYFSFEDRRWIFKAVTPEGKILTLFRAMETPIKRHIKIKGEATPYTPGMEIYFERR
                     LDLIWKGKSKKMKTVAQLWKRQGKHCPQCGQPITNQTGWNIHHRIRKVMGGSDELTNI
                     ELLHPNCHRQLHSREAGAHRKHL"
BASE COUNT          242 a          244 c          235 g          148 t
ORIGIN      
        1 atccggggat gggctaccta tcaccgacac gtcgtggcga aagaaacatt taattatgtc
       61 gacacccaaa tctggcgagc tatatggcga tggtgtgtaa gacggcaccc ccgaaaagga
      121 ttgcggtgga tcgccggacg atacttctcc ttcgaagaca gacgctggat ctttaaggcc
      181 gtaaccccgg aagggaaaat tctgactctc ttccgggcaa tggaaacacc gataaagcgc
      241 catataaaaa tcaaaggcga agcaacacca tacacacccg gaatggaaat ctacttcgaa
      301 cgtcgtctgg atctaatctg gaaaggcaaa tcaaagaaga tgaaaacagt agctcaactc
      361 tggaaaaggc aggggaaaca ctgtccgcag tgcggacaac ccataaccaa ccagaccgga
      421 tggaatatac atcaccggat caggaaagtt atgggcggca gtgacgaact gaccaatatc
      481 gagctgttac atccgaactg ccaccgacag ttacacagca gggaagccgg tgctcaccga
      541 aagcacttat aaaggcctga gcggagtgcg gggaaactcg cacgctccgt tcttaggggg
      601 cggcggcgca gcaatgtgct gcagctaccc gaccaacaaa aaacagccag aaacggtgac
      661 agtgtcacgg aacatcacac ctgttcctgg catacagcca tacactacct tctgaccgga
      721 aagccgggcc ggcagcgccg caggcgctgc accccgcgtt tccggcaggc gtcagcctgc
      781 caggggggtt cgcgcgggct gacaggcggc ctccagcgcc tgcccctgta tcacctgttt
      841 ctgctgccct gaaggatgta gtgaccacc
//
LOCUS       DAKISA010000313          862 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.321, whole genome shotgun sequence.
ACCESSION   DAKISA010000313 DAKISA010000000
VERSION     DAKISA010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.321"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            63..443
                     /locus_tag="OVW45_004622"
     CDS             63..443
                     /locus_tag="OVW45_004622"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCV5528871.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPKTLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            440..787
                     /gene="tnpB"
                     /locus_tag="OVW45_004623"
     CDS             440..787
                     /gene="tnpB"
                     /locus_tag="OVW45_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCV5528872.1"
                     /translation="MIPLPSGIKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTSDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
BASE COUNT          216 a          233 c          222 g          191 t
ORIGIN      
        1 aatctgacga attagacaaa gtggtgtcca ccaaataagt agtgggaacc aaagtgtcag
       61 atatgcagaa aaatgtgact cccggcaggc gaaaaggctg ccctaattat cctcccgaat
      121 ttaaacagca gctcgttgct gcctcctgtg aacccgggat atccatctca aaacttgctc
      181 ttgaaaatgg cattaacgcc aatctgttgt tcaaatggcg acaacaatgg cgcgagggaa
      241 agctgctatt accttcttca gagagccccc agctacttcc tgtgactctc gatgcagctg
      301 ccgaacagcc agaatcgctc gcagaggacc cgaaaaccct cagtatcagc tgtgaggtaa
      361 cgttccggca cgggacgctc cgcttcaatg gcaatgtcag cgaaaagctc ctgactctgc
      421 tgatacagga actgaagcga tgatcccgtt accttccggg atcaaaattt ggctggttgc
      481 cggtatcacc gatatgagaa atggcttcaa cggcctggct gcgaaagtac agacggcgct
      541 gaaagacgat cccatgtccg gccatgtttt cattttccgg ggccgcagcg gcagtcaggt
      601 taaactgctg tggtccacca gtgacggact gtgcctcctg accaaacggc tggagcgtgg
      661 gcgcttcgcc tggccgtcag cccgtgatgg caaagtgttc cttacacagg cgcagctggc
      721 gatgctgctg gaaggtatcg actggcgaca gcctaagcgg ctgctgacct ccctgaccat
      781 gctgtaaatc tctttatcct ggttgtcaca gaataagccc ggtaaaatac gggcttatga
      841 acgacatctc ttctgacgac at
//
LOCUS       DAKISA010000314          857 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.322, whole genome shotgun sequence.
ACCESSION   DAKISA010000314 DAKISA010000000
VERSION     DAKISA010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.322"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(1..516)
                     /locus_tag="OVW45_004624"
                     /pseudo
     CDS             complement(1..516)
                     /locus_tag="OVW45_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
BASE COUNT          274 a          187 c          160 g          236 t
ORIGIN      
        1 tgggttgctg gcatcattgc ctttcgcatc acccggacag ttagaagagg ctacccacca
       61 agcattttta tataccgcac cattaaagac gacataccag gtctgaccgc cctgctgttt
      121 attccaggca actacagaag aattagacgg aacagtgccc tgtgctggtg tagccgaacc
      181 gccattagcc ggcgtattgg atgcaggcgt atttgaattt gaagaagatg agctgccgct
      241 cttttcgcag gaaagtgtat tgccaaactg actaatttca gcagctgttg cggtacgctt
      301 taaacgccac gggttagttg catcatttgc tttcgctttt cccgggcaat ttgtagaaaa
      361 accccactag gcattttcat aaattttgcc atcgaaaata acctgatatt tattaccacc
      421 ttgttggtta ttccatgctt ccattgccaa tgctggcaga gcggaacaca ccagccccat
      481 accaatcata gatttagtaa atatatttaa tttcattata atcccttgtg acgtaaaaac
      541 tgcaaaacaa aagtcctacg caggaattat ttacgtttta cgagaaccct tcaatatgaa
      601 ttaaagtgag gcattaaaaa taagaaaaaa gcctgacaaa tgaagaattt taaaaacaga
      661 aacattcata tttaaaatat taaattgaat tgatatttta aatatgaata atttattcgt
      721 tctgacagta cgaataagat atgcagtcaa caaatgcaaa aagggcgccg aagcgccctt
      781 ttcaattcaa aactaattaa cgtgtaatta gcccagaact ttagcaacaa cgcccgcgcc
      841 aacggtacgg ccgcctt
//
LOCUS       DAKISA010000315          854 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.323, whole genome shotgun sequence.
ACCESSION   DAKISA010000315 DAKISA010000000
VERSION     DAKISA010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.323"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..>854
                     /locus_tag="OVW45_004625"
     rRNA            <1..>854
                     /locus_tag="OVW45_004625"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          237 a          188 c          262 g          167 t
ORIGIN      
        1 ccgtacacaa aaatgcacat attgtgagct cgatgagtag ggcgggacac gtggtatcct
       61 gtctgaatat ggggggccca tcctccaagg ctaaatactc ctgactgacc gatagtgaac
      121 cagtaccgtg agggaaaggc gaaaagaacc ccggcgaggg gagtgaaaaa gaacctgaaa
      181 ccgtgtacgt acaagcagtg ggagcacgct taggcgtgtg actgcgtacc ttttgtataa
      241 tgggtcagcg acttatattc tgtagcaagg ttaaccgaat aggggagccg aagggaaacc
      301 gagtcttaac tgggcgttaa gttgcagggt atagacccga aacccggtga tctagccatg
      361 ggcaggttga aggttgggta acactaactg gaggaccgaa ccgactaatg ttgaaaaatt
      421 agcggatgac ttgtggctgg gggtgaaagg ccaatcaaac cgggagatag ctggttctcc
      481 ccgaaagcta tttaggtagc gcctcgtgaa ttcatctccg ggggtagagc actgtttcgg
      541 caagggggtc atcccgactt accaacccga tgcaaactgc gaataccgga gaatgttatc
      601 acgggagaca cacggcgggt gctaacgtcc gtcgtgaaga gggaaacaac ccagaccgcc
      661 agctaaggtc ccaaagtcat ggttaagtgg gaaacgatgt gggaaggccc agacagccag
      721 gatgttggct tagaagcagc catcatttaa agaaagcgta atagctcact ggtcgagtcg
      781 gcctgcgcgg aagatgtaac ggggctaaac catgcaccga agctgcggca gcgacgctta
      841 tgcgttgttg ggta
//
LOCUS       DAKISA010000316          852 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.324, whole genome shotgun sequence.
ACCESSION   DAKISA010000316 DAKISA010000000
VERSION     DAKISA010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.324"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            2..598
                     /locus_tag="OVW45_004626"
     CDS             2..598
                     /locus_tag="OVW45_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HCV5528873.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCTLSPTINDHFAFHPLSRRIIAFEAIVQKNEAHQP"
     gene            595..>852
                     /locus_tag="OVW45_004627"
     CDS             595..>852
                     /locus_tag="OVW45_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCV5528874.1"
                     /translation="MAVGQRKDGEIYTADLKSKALAFAMAHALELGDKMISINLLPMT
                     LVNEPDAVSFLLDEIKANALVPEQIIVEFTESEVISRFDEFA"
BASE COUNT          240 a          190 c          182 g          240 t
ORIGIN      
        1 aatgcttacc acccttattt atcgtagcca tatacgtgac gacgaacctg tcaaaaaaat
       61 cgaagaaatg gtttcgatag caaatcgcag gaacatgcag tctgacgtaa cagggatctt
      121 actgtttaat ggttctcatt ttttccagct tctggaaggt ccggaagaac aggttaaaat
      181 gatatatcgg gctatatgcc aggatccacg gcactataat attgttgagc tgctgtgcga
      241 ttacgcgcct gctcgccgtt ttggcaaagc gggaatggaa ttatttgatt tgcgcctgca
      301 cgagcgagat gacgttttac aggccgtatt cgacaaaggc acatcaaaat ttcagctaac
      361 ttatgatgac agagcgctac aattttttcg tacttttgtc cttgcaaccg aacaatcaac
      421 ctatttcgag atccctgccg aagactcctg gctttttatc gctgacggat ctgataaaga
      481 acttgattcc tgtacccttt caccaactat aaacgaccac tttgcctttc atcccttatc
      541 gcggcggata atcgcttttg aagccattgt gcaaaaaaat gaagcccatc agccatagcg
      601 gttgggcagc gtaaagacgg ggaaatctac acagcggatc tcaaaagtaa ggcgcttgca
      661 ttcgcgatgg cacacgcact tgagctcggt gataaaatga tttcaatcaa tctattacct
      721 atgaccctgg ttaacgaacc tgacgcagtc tcttttttac ttgatgaaat aaaggccaat
      781 gctctggtgc ctgaacaaat catcgttgaa tttactgaaa gtgaagttat atctcggttt
      841 gatgagtttg ca
//
LOCUS       DAKISA010000317          839 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.325, whole genome shotgun sequence.
ACCESSION   DAKISA010000317 DAKISA010000000
VERSION     DAKISA010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.325"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            173..>839
                     /gene="istA"
                     /locus_tag="OVW45_004628"
     CDS             173..>839
                     /gene="istA"
                     /locus_tag="OVW45_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family transposase"
                     /protein_id="HCV5528875.1"
                     /translation="METCFKILQLKFDKKLTNRCIGLTLHISASTVFEVLARFKASSL
                     SWPLPADISHDTLEKLIFPPKDTSASELVMPDMLYFDTEMRKPGVTRQLLWMEYKAQA
                     GDKAMGYSHFCRCYRKWKKTRRLSMRQEHRAGEKLFIDFCGPTVPVINPDTGEIRRVA
                     IFVAVMGASNYTYVEACEGQDMMSWLNAHSRCLTFLGGVPKLLIPDNLRSAVKKADRY
                     EPVI"
BASE COUNT          213 a          223 c          199 g          204 t
ORIGIN      
        1 ccatctccga tcacagtttg gcatcacaat cgatcacgat ttggcatact tccgatcatt
       61 gattggaatt cgccagtcac tccgggaatt aactcttttc accacagtct ccattgccgt
      121 gtttaaacca gtggagacgt caatgtccaa aaagagaaaa tccaggagca ctatggaaac
      181 ttgttttaag atccttcagc tcaagttcga taagaagctg actaaccgtt gtatcggact
      241 gacgttacac atcagcgcct caaccgtttt tgaggttctt gcccgcttca aagcctcttc
      301 tctgtcatgg ccattacctg cggatatatc ccatgacacg ctggagaaac tcattttccc
      361 gcccaaagac acctctgcgt cagagcttgt tatgcctgac atgctgtact tcgatacgga
      421 aatgagaaaa cctggcgtga cgcgccaact gttgtggatg gaatacaagg cacaggcggg
      481 tgacaaagcg atgggatact cacacttctg ccgttgctac cggaagtgga aaaaaacgcg
      541 acgcctctcc atgcgacagg agcatcgtgc tggcgaaaag ctctttatcg acttctgtgg
      601 tcctacggtc cctgttatta atccggacac cggagagata cgccgcgtgg caatcttcgt
      661 ggctgtaatg ggggcgtcca actataccta tgttgaagcg tgcgaaggtc aggacatgat
      721 gtcatggtta aatgcccaca gccgttgcct gaccttcctg ggcggcgtac cgaagctgct
      781 catccccgat aacctcagaa gcgcagtgaa aaaagccgat cgctatgagc ctgtcatca
//
LOCUS       DAKISA010000318          835 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.326, whole genome shotgun sequence.
ACCESSION   DAKISA010000318 DAKISA010000000
VERSION     DAKISA010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.326"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            2..757
                     /gene="istB"
                     /locus_tag="OVW45_004629"
     CDS             2..757
                     /gene="istB"
                     /locus_tag="OVW45_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_039272515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family helper ATPase
                     IstB"
                     /protein_id="HCV5528876.1"
                     /translation="MSDNLLNKLTQLKLPAMAGSLIRQRETPQTYDELSFEERLTLLV
                     DDELLSRENSRVARLRKNACLKYQATPEGLRYPASRGLRAEQMRELLNGHYIIHRKNL
                     LITGPTGCGKSWIANALGEQACRQKYSVRYCRTGRLLEQLAQGRVDGSWLKYLKQLQK
                     IQVLILDDLGLEQLSNAQCNDLLEITEDRYGQSSTIVVSQFPVDKWHGLMENPTTADA
                     ILDRLVHNSHRVVLQGESLRKNPPTVESSEKTS"
BASE COUNT          256 a          185 c          236 g          158 t
ORIGIN      
        1 tatgagtgat aatctgttaa acaaactgac ccaactgaaa ctcccggcga tggccgggag
       61 cctgatacgg caacgggaaa cgcctcagac ttacgacgaa ctgtcgttcg aggaaaggct
      121 gacactgctg gttgatgacg agcttctgag cagagaaaac agtcgtgtgg cacggttgag
      181 aaaaaatgcc tgcctgaaat atcaggcaac accggaaggg ctgcgttatc cggcctcaag
      241 aggtctgcgg gctgaacaga tgagggaact gctgaacggc cactacataa tccacaggaa
      301 aaacctactg ataaccggtc cgacaggatg cggtaagagc tggatagcca atgcgctggg
      361 cgaacaggca tgtcgacaga aatacagcgt ccggtactgt agaaccgggc gattactgga
      421 acagttggct cagggacgtg ttgatggcag ttggctgaaa tatctgaaac agttgcaaaa
      481 aatacaggtt ctaatcctgg acgatctggg gctggaacag ttaagtaacg cccagtgtaa
      541 tgaccttctg gagatcacag aggatcgtta cggacaaagc agcacgatag tggtaagcca
      601 gttcccggta gataaatggc atggtctgat ggagaaccca acaacagcag atgccatcct
      661 ggacagactg gtacataact cacacagagt ggtccttcag ggagagtccc tgaggaaaaa
      721 tcccccgaca gtggaaagca gcgaaaaaac gagttaaaaa gcggtagaaa aagttaagtt
      781 cccggttgat cgaatggatg cgaatcgctg atcgaacttc gatgccaatc cgtga
//
LOCUS       DAKISA010000319          819 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.327, whole genome shotgun sequence.
ACCESSION   DAKISA010000319 DAKISA010000000
VERSION     DAKISA010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.327"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..>819
                     /gene="istA"
                     /locus_tag="OVW45_004630"
     CDS             <1..>819
                     /gene="istA"
                     /locus_tag="OVW45_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element ISSso4 family transposase"
                     /protein_id="HCV5528877.1"
                     /translation="IPARPRKPKDKPKAENGVLIVERWLLARIRNETFHTLRALNARL
                     RELLTDMNNRPMKGYGNQTRAERFRMLDAPALSPLPLEPYEYTEYKAVKVGPDYHVEY
                     ARHWYSVPHELVGQRLSLKVGQSVVQLWHKGQCVAQHPRSTHEYKHTTNPLHMPERHR
                     RHGTWTPERLIEQGNRTGPSTGRVVESMLKAKPHPELAYRAVLGLLALQKKYGPERLE
                     KACYVALHYNAPDRRFIDNLLRHHRDNVELPLSRQGEQHPAYASEHENLRGPGY"
BASE COUNT          206 a          247 c          213 g          153 t
ORIGIN      
        1 tatcccggcc cgcccgcgca aacctaaaga taaaccaaaa gcagaaaacg gcgtcctcat
       61 cgtcgagcga tggcttcttg cccgcatccg taatgaaacc ttccacacgc tcagggcgct
      121 gaatgcacgc cttcgggagt tgctgacgga catgaataac cgaccaatga agggatacgg
      181 taatcaaacc cgcgcagaac gcttccgcat gctggatgca ccagcactct ctccgctgcc
      241 tctggagcct tatgaataca cggaatacaa agccgtgaag gttggccctg actaccacgt
      301 tgaatatgcc cgtcactggt actccgttcc acacgaactg gtcggtcagc gtctgtcact
      361 gaaggtgggg cagtcggtgg tacagctctg gcacaagggg cagtgtgtgg cgcaacatcc
      421 gcgcagcaca catgaatata aacacacaac caatccgctt cacatgccag aacgccaccg
      481 gcggcatggg acatggacac cggaacgcct tatcgagcag ggaaaccgta caggtccgtc
      541 cacaggccgg gtggttgaat ccatgctgaa agccaaaccc catccggagc tggcttaccg
      601 tgcagtcctg ggtctgctgg cacttcagaa aaaatacggc ccggaacggc tggaaaaggc
      661 ctgttacgtt gccctgcact ataacgcccc ggacagacgc tttatcgaca atctgctgcg
      721 tcatcatcgg gataacgttg agcttccgtt atcccgccag ggcgaacaac atccggcata
      781 tgcttcagag catgaaaacc tgcgtggtcc tggctacta
//
LOCUS       DAKISA010000320          811 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.328, whole genome shotgun sequence.
ACCESSION   DAKISA010000320 DAKISA010000000
VERSION     DAKISA010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.328"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            1..811
                     /locus_tag="OVW45_004631"
                     /pseudo
     CDS             1..811
                     /locus_tag="OVW45_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
BASE COUNT          224 a          199 c          217 g          171 t
ORIGIN      
        1 tgaaggcgct tcccgattat ctcttcctga aggcacttta ggacaatggg ttaccgccgc
       61 cagaaaaggg ctcggtactc ctggttcccg cacggtggct gaactggaat ctgaaattct
      121 gcaactgcgt aaggcgttaa atgaagctcg ccttgagcga gatatattaa aaaaagcaac
      181 agcgtatttt gcacaggagt cgctgaaaaa tacgcgttaa tcgaacaatg gcgacaacaa
      241 tttcccattg aagcgatgtg tcaggtattt ggtgtatcca ggagcggtta ttacaactgg
      301 gtacagcatg aaccctcaga cagaaaacaa agtgatgagc ggctaaaact ggagattaag
      361 gtggcacata tccgcactcg cgaaacatat ggaacccggc ggctccagac ggagctggca
      421 gagaatggca tcatcgttgg tcgtgaccga ctggcacgtc ttcgtaagga gctaaggcta
      481 cgctgtaagc agaaacgcaa gttcagagcg actacgaact cgaaccacaa tctgccagtt
      541 gcgccaaatc tgctgaacca gacgttcgct cctacagcac caaatcaggt ctgggtggcg
      601 gacctgacgt atgttgccac acaggaggga tggttgtacc tcgctggcat caaagatgtt
      661 tatacgtgcg aaattgtcgg ctacgccatg ggagagcgca tgacaaaaga gctgacaggt
      721 aaagccctgt ttatggcgct caggagccag cgcccacctg ccgggctaat ccaccactct
      781 gatcgaggtt cacagtactg cgcatacgat t
//
LOCUS       DAKISA010000321          790 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.329, whole genome shotgun sequence.
ACCESSION   DAKISA010000321 DAKISA010000000
VERSION     DAKISA010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.329"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..>790
                     /locus_tag="OVW45_004632"
     CDS             <1..>790
                     /locus_tag="OVW45_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HCV5528878.1"
                     /translation="ECQIRNSNFDNCYLYASHFTRAEFLSAKEISFIKSNLTAVMFDH
                     VRMSTGNFKDCITEQLELTIDYSDIFGNEDLDGYINNIIKMIDTLPDNAMILKSVLAV
                     KLVMQLKILNIVNKNFIENMKKTFSHCPYIKDPIIRSYIHSGEDDKFDNFMRQHRFSK
                     VNFDTQQMIHFINRFNMNKGLIDKNNNFFIQLIDQALRSTDDMIKANAWYLYKEWIRS
                     DDVSPIFIETEEKLRTFNTNKLTRNDNIFILFSSVDDGPVMVVSS"
BASE COUNT          281 a          115 c          134 g          260 t
ORIGIN      
        1 cgaatgtcag attagaaatt caaacttcga taattgttat ctttacgctt cgcacttcac
       61 cagagcagag tttctgtctg ccaaagaaat atcatttatt aaatcgaatt tgacagctgt
      121 tatgtttgat catgtgcgaa tgtcgacagg gaattttaaa gattgcatta cagaacaatt
      181 ggaattaact attgattatt cagatatatt tgggaatgaa gatctcgatg gttatatcaa
      241 taacattata aaaatgattg atacattgcc agataatgca atgatattga aatccgttct
      301 ggcagtaaaa ctggtgatgc aattaaaaat tctcaatatt gttaataaaa actttattga
      361 gaatatgaaa aaaacattta gccattgtcc ttatataaaa gatccaatta tacgtagtta
      421 tatacattct ggggaagatg acaagttcga taattttatg cgtcaacatc gattcagtaa
      481 ggtgaatttc gatacccaac agatgatcca ttttattaac agatttaata tgaataaagg
      541 gctgattgat aaaaataaca atttttttat ccaacttatc gatcaggcct tacgatcaac
      601 ggatgatatg atcaaagcaa atgcctggta tctttataaa gagtggattc gtagtgatga
      661 tgtttcacct atatttatag aaactgaaga aaagttaaga acctttaaca ctaataaatt
      721 aacacgaaac gataatatat ttatcctgtt ctcctctgtc gatgatgggc ctgttatggt
      781 ggtaagctcc
//
LOCUS       DAKISA010000322          788 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.330, whole genome shotgun sequence.
ACCESSION   DAKISA010000322 DAKISA010000000
VERSION     DAKISA010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.330"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            211..>788
                     /locus_tag="OVW45_004633"
     CDS             211..>788
                     /locus_tag="OVW45_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc20 family transposase"
                     /protein_id="HCV5528879.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     EAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIR
                     GLLTQIHPALERVLGPRLEHPAVLDLLQRYPSP"
BASE COUNT          172 a          237 c          205 g          174 t
ORIGIN      
        1 ccttataatg gggtgttagc cagccagacc cggcatgatt actgccccca gtcgtccatg
       61 atccgggggg tgatgtcacc gggtctggtg gggcgctggt aaccgctaat aggggtcagg
      121 tcaggcactt ttgccgggac cgtctgtaac gtggatgccg gtacctgctc cccgtggtta
      181 tctggttaac ccatatacaa gggagacaga atgaccgaat ccagcgatta cgaatccgtc
      241 caggtcttta tcggcgttga tgtcggtaaa gatacgcatc acgctgttgc cattaatcgt
      301 tcaggtaaac gcctgttcga taaagcatta cccaacgacg aaaacaaact caggtcgcta
      361 atatctgacc tgaaacaaca tggtcagata ctgctggttg ttgatcagcc agctaccatc
      421 ggtgcgttac ctgtcgccgt tgcccgctca gaaggagtcc ttgtcggata cctccctgga
      481 ctggccatgc gccgcatagc cgacttacac gccggtgaag ctaaaactga tgctcgtgac
      541 gctgccatca ttgccgaagc tgcccgtacc ctgcctcacg cgctacgcac gctgaaactg
      601 gctgacgagc aaatcgccga actctccatg ctctgcggct tcgatgatga tcttgccgca
      661 cagacaacgc aggccagcaa ccgtatccgc ggccttctga cccagataca tccggcactg
      721 gagcgcgttc tcggtccgag acttgagcac ccggcggtac tcgatcttct ccagcgatat
      781 ccctcacc
//
LOCUS       DAKISA010000323          755 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.331, whole genome shotgun sequence.
ACCESSION   DAKISA010000323 DAKISA010000000
VERSION     DAKISA010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.331"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..185)
                     /locus_tag="OVW45_004634"
     CDS             complement(<1..185)
                     /locus_tag="OVW45_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528880.1"
                     /translation="MLEINTSKIKNTVTFSVDKDSLKKAKDSITGLKEFAENIKPAKL
                     RFDNVTKGYKKAQSEVD"
     gene            complement(200..403)
                     /locus_tag="OVW45_004635"
     CDS             complement(200..403)
                     /locus_tag="OVW45_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000452041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528881.1"
                     /translation="MEVILKSKNGVHVHLDANDSRGLLNLKYLCSLLDVPYEGVKARM
                     FRLNESIDQALHHFLSKEGGKND"
     gene            complement(492..>755)
                     /locus_tag="OVW45_004636"
     CDS             complement(492..>755)
                     /locus_tag="OVW45_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HCV5528882.1"
                     /translation="IKVTDPLHLAHMTADAVGVPKFAKGSNVYQNIYGAASSTFELIN
                     AAPAEVYSYSYESSRGDAVNVVTENSQMVVNHGVGFSVQITVK"
BASE COUNT          225 a          156 c          124 g          250 t
ORIGIN      
        1 ttgtctactt cgctttgtgc tttcttatag cctttagtga cattatcaaa ccttaattta
       61 gctggcttga tattttctgc gaattccttt aagcctgtaa tagagtcttt cgcttttttc
      121 aagctgtctt tatctacact aaatgtaact gtgttcttaa ttttagaagt gttaatttct
      181 aacatttaaa atccccttat taatcatttt taccaccttc tttgcttaaa aaatggtgta
      241 gagcctgatc aatactctcg tttaatctga acatacgggc ttttacgcct tcatatggaa
      301 catctaagag agaacagaga tatttcagat ttaacaagcc tcttgaatca ttagcatcta
      361 aatgtacgtg tacaccattt tttgatttca gaatcacttc catattttaa tcacctctca
      421 aaatttaaaa aaataaataa aaaaaaggcg aagcgtgaat acacgccacg cctctattac
      481 acgttaaaat attatttaac agtgatttga acggagaagc ctacaccgtg gtttaccacc
      541 atctggctat tctctgtaac aacgttaaca gcatcaccac gagaagattc atagctatag
      601 ctatacacct cagcaggagc ggcattaatc agttcaaagg tgctggaagc agccccgtag
      661 atgttctgat atacgttaga gcctttagca aacttcggta cgcctacagc gtctgcggtc
      721 atatgtgcaa ggtgtagcgg atcagtcact ttgat
//
LOCUS       DAKISA010000324          745 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.332, whole genome shotgun sequence.
ACCESSION   DAKISA010000324 DAKISA010000000
VERSION     DAKISA010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.332"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..396)
                     /locus_tag="OVW45_004637"
     CDS             complement(<1..396)
                     /locus_tag="OVW45_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCV5528883.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLAWSRL"
BASE COUNT          233 a          174 c          108 g          230 t
ORIGIN      
        1 caacctggac catgctaaat tcacaccgtg ctgcgcggcc tgattaaata gtgcatacgc
       61 tttggcataa tccacccgca cctgctcacc gtcgtagtag atacccccca gcgctaacgg
      121 agcttctctg tcaccttgtt tagccgctaa ctcgaaccat ttaatcgctt cctgaacatc
      181 aggtttggta tcataacgag gctgataata ttcccagcct aacaggctct gcgcgtcgat
      241 atctcccgat tcagccattt tatgcagtgt atttataaca tcgggagcag gtttaccacc
      301 gtatatgctg tcatacaatt catgcgcttt ctcgctggcc accacgcttg gtgaaactat
      361 cagcattaca gaaaataata ttcttttagt taacatgtat acctttatgt cctcactgtc
      421 atatatttcc ataataatta accaccaaat atccatttaa tgaataaata cagtgccata
      481 aaggtccaag taataagcac acatttagcc tgaaacatta tcatccatgt caccaaatcg
      541 tttatttagt gagataaaac tagcatattc gtaagataaa aaatgcacat ggcaatcaag
      601 aaatgatatt attacttttt tgatgtaaaa ctatttagtt ttaaaattca ttttttcaac
      661 atatagagca ttcattttat gaatgcaaaa aaacatacct gacacacaaa ttactctcct
      721 ctgtaatatt ttctcattaa cactc
//
LOCUS       DAKISA010000325          727 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.333, whole genome shotgun sequence.
ACCESSION   DAKISA010000325 DAKISA010000000
VERSION     DAKISA010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.333"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(158..>727)
                     /locus_tag="OVW45_004638"
     CDS             complement(158..>727)
                     /locus_tag="OVW45_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCV5528884.1"
                     /translation="FFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIV
                     IAGILLNVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFF
                     IAVVLGMIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQ
                     IHLLDKYRKVPPEPVHADIPVGAVSDVKA"
BASE COUNT          226 a          179 c          157 g          165 t
ORIGIN      
        1 tggcttgata accggataac aacgtgcgct ttgttgatgc cggatgcggc gtgaattcct
       61 tatccggtct acaaaatttg caaatccaaa gcattacatt aagacgtggc ctgataaaca
      121 tatcgcatca ggccattttc gttagctatt gtccacgtca ggctttcaca tcactcactg
      181 caccgaccgg aatatcagca tgaacaggct cgggcggtac tttgcgatat ttatccagca
      241 gatgaatctg gatcctgcgc agcgtgtcgc cattgaggcg atagaaacgg aagtagagaa
      301 tcagcgttac catgaaaaac agagttggta gagcaatcat aataaactgc atacctaata
      361 gggcttgcgt agactgttca acattcggta catagccaat catccctaac acaaccgcaa
      421 tgaaaaaagc cgcaaaggct gaaccgccct tcaccaccat agtctgcacg gagtaagcga
      481 tactttcaca gcgtacgtgc agtttatatt caccgtaatc aacggtatct gccaccatga
      541 tgacctgtaa tacccagaaa agcgccgttc ccacattcag caaaataccc gcaatcacaa
      601 tgaggacgac gttgtgatag ctcattaatg ccattaacag gagaacacca cagcttaaca
      661 ccggaagaat agatgctccg gcccataaaa tgcgtcggga taatgattta actaagcgtg
      721 ggaagaa
//
LOCUS       DAKISA010000326          726 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.334, whole genome shotgun sequence.
ACCESSION   DAKISA010000326 DAKISA010000000
VERSION     DAKISA010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.334"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            35..726
                     /locus_tag="OVW45_004639"
                     /pseudo
     CDS             35..726
                     /locus_tag="OVW45_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
BASE COUNT          167 a          183 c          205 g          171 t
ORIGIN      
        1 atttagtgta tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc
       61 ctcctgttca gctactgacg gggtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcactgcc gtaaaacatg gcaactgcag ttcacttaca ccgcttctca
      181 acccggtacg caccagaaaa tcattgatat ggccatgaat ggcgttggat gccgggcaac
      241 tgcacgcatt atgggcgttg gcctcaacac gattttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acagccgggc agtgacgtca tcgtctgcgc ggaaatggac
      361 gaacagtggg gctatgtcgg ggctaaatcg cgccagcgct ggctgtttta cgcgtatgac
      421 agtctccgga agacggttgt tgcgcacgta ttcggtgaac gcactatggc gacgctgggg
      481 cgtcttatga gcctgctgtc accctttgac gtggtgatat ggatgacgga tggctggccg
      541 ctgtatgaat cccgcctgaa gggaaagctg cacgtaatca gcaagcgata tacgcagcga
      601 attgagcggc ataacctgaa tctgaggcag cacctggcac ggctgggacg gaagtcgctg
      661 tcgttctcaa aatcggtgga gctgcatgac aaagtcatcg ggcattatct gaacataaaa
      721 cactat
//
LOCUS       DAKISA010000327          720 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.335, whole genome shotgun sequence.
ACCESSION   DAKISA010000327 DAKISA010000000
VERSION     DAKISA010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.335"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            1..317
                     /gene="tnpA"
                     /locus_tag="OVW45_004640"
                     /pseudo
     CDS             1..317
                     /gene="tnpA"
                     /locus_tag="OVW45_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604943.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          236 a          158 c          128 g          198 t
ORIGIN      
        1 atgacgcgac agaaaaacta cgcacttact tttcaaatgt atgtgctgat tttgaagctg
       61 aactggttga aatggatggc gaaccagatc acgtccattt gttaattaac tatcctccca
      121 aactggcgat atccagtctg gtaaacagcc tcaaaggcgt atcgggtagg tgactgcgac
      181 gagatcgacc agatattgca gtcaggtatt actacaaagg cgttttgtgg agtgttggct
      241 attttgccag tagctgcgga ggtgcgccaa tatccgtcat ccgccaatac attgaacaac
      301 agcaaacacc tggttaggtg gaaaaccacg ccttatatcc ctgccctgaa ggacggggtt
      361 ttacggcgca ccggataagc ctggagatta tctacaccag catggacatc attgtactat
      421 gccccgtaca tgccccatca catcctcaac atcaaagtat tagagccttg caaaccatta
      481 tgcgtctcag tttgtctcat ctatcttacg caaaaaaaaa tagaactaat atcaagcggc
      541 gctatattgc gtattaaata aaaccacctc aattacatta ataaatctaa acaaacatca
      601 tgcaaataat ttccatggtt atttctaggc tatttaaatt aaataaataa attatgagtc
      661 aatcataaat aacaaccttt aatataattt aactaccaaa tttatattcg ttcacaaaat
//
LOCUS       DAKISA010000328          713 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.336, whole genome shotgun sequence.
ACCESSION   DAKISA010000328 DAKISA010000000
VERSION     DAKISA010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.336"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            69..>713
                     /locus_tag="OVW45_004641"
     CDS             69..>713
                     /locus_tag="OVW45_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCV5528885.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARNAEGQD
                     NTRNILDPKTFLEFLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLSAFTTMGTRQGHTAIIFQQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCKDSVDSTITD"
BASE COUNT          240 a          110 c          143 g          220 t
ORIGIN      
        1 tttttgatat ttttttgtga gtaaaatttg taatgcttag acgttcttat taactcaagg
       61 agttcgtcat gagcaaaata tcagatttga attattctca acacattaca ttagccgaca
      121 attttaaaca aaaaagtgaa gttttaaata cctggcgtgt tggaatgaat aattttgccc
      181 gtaatgccga ggggcaggat aacacaagaa atatccttga tcctaagaca tttttggaat
      241 ttttggtaaa aatatttact ctaggttatg tggattttag caaacgctcc aacgaagcgg
      301 gaagaaatat gatggctcat attgagtcct catcttatat caaaaataat gatggcagtg
      361 agataatgaa gtttgttatg aataatcctg aaggggaacg agcggattta tctaaggtgg
      421 aaatagaaat cacactttcc gctttcacta ctatggggac tcgtcaagga catacggcta
      481 ttatatttca acaacctgat ggttcgacta atcgttatga aggaaagtcc tttgaaagaa
      541 aagatgagag ttcattacac ctgattacta acaagattct ggcatgttac caacgcgaag
      601 ctaacaagga aatagctcgt ctattaaata ttccgcagga gttaaataat tctcaggact
      661 tgaataactc gcaggtgagt tgtaaagatt cagttgattc tacgattacg gat
//
LOCUS       DAKISA010000329          695 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.337, whole genome shotgun sequence.
ACCESSION   DAKISA010000329 DAKISA010000000
VERSION     DAKISA010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.337"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..>695
                     /gene="tuf"
                     /locus_tag="OVW45_004642"
     CDS             <1..>695
                     /gene="tuf"
                     /locus_tag="OVW45_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCV5528886.1"
                     /translation="VDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLG
                     RQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKALEG
                     DAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKV
                     GEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPG
                     TIKPHTKFESEVY"
BASE COUNT          162 a          167 c          205 g          161 t
ORIGIN      
        1 gtagactgcc cggggcacgc cgactatgtt aaaaacatga tcaccggtgc tgctcagatg
       61 gacggcgcga tcctggtagt tgctgcgact gacggcccga tgccgcagac tcgtgagcac
      121 atcctgctgg gtcgtcaggt aggcgttccg tacatcatcg tgttcctgaa caaatgcgac
      181 atggttgatg acgaagagct gctggaactg gttgaaatgg aagttcgtga acttctgtct
      241 cagtacgact tcccgggcga cgacactccg atcgttcgtg gttctgctct gaaagcgctg
      301 gaaggcgacg cagagtggga agcgaaaatc ctggaactgg ctggcttcct ggattcttac
      361 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
      421 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      481 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      541 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
      601 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      661 aagccgcaca ccaagttcga atctgaagtg tacat
//
LOCUS       DAKISA010000330          684 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.338, whole genome shotgun sequence.
ACCESSION   DAKISA010000330 DAKISA010000000
VERSION     DAKISA010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.338"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            1..368
                     /locus_tag="OVW45_004643"
                     /pseudo
     CDS             1..368
                     /locus_tag="OVW45_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          194 a          172 c          175 g          143 t
ORIGIN      
        1 cggtggcgca gatgcaaagc cagatggacc cggtatacac gccggcgcga aaaattcacc
       61 tgtaccactg cgaccatcgc ggcctgccgc tggcccttat cagcacggaa gggacaacag
      121 cgtggtgcgc agaatatgat gaatggggca acctgctgag tgatgagaac ccgcatcatc
      181 tgcagcagct cattcgcctg ccggggcagc agtatgatga ggagtccggc ctgtattaca
      241 accgccaccg ctattatgac ccgctgcagg ggcggtatat cactcaggat ccgattggac
      301 tgaagggggg atggaatttt tatcagtatc cgttgaatcc ggttcagtat atagattcaa
      361 tggggctggc atctaaatat ggacacttaa ataatggcgg atatggagcg aaacccagca
      421 aaccgcctac gcccgatcca agtaagttgc cagacatagc gaaacaatta agactgccat
      481 atcctattga ccaggccagt agtgcgccta atgttttcaa aacattcttc agagcattaa
      541 gcccttacga ttacacactg tattgcagga agtgggtaaa acccaatctg acttgtatgc
      601 cgcaggatga tccccagtat ccagggatgg agacaaagac agcaagtgat tacctgccac
      661 agacaaattg gtcaacaact caat
//
LOCUS       DAKISA010000331          671 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.339, whole genome shotgun sequence.
ACCESSION   DAKISA010000331 DAKISA010000000
VERSION     DAKISA010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.339"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(372..512)
                     /locus_tag="OVW45_004644"
     CDS             complement(372..512)
                     /locus_tag="OVW45_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528887.1"
                     /translation="MAESGYGLAGLGVGKVKSINPYRLTPGFGGFTPVSYATTEYRLT
                     CR"
BASE COUNT          177 a          155 c          201 g          138 t
ORIGIN      
        1 ggcggccgga tggtataccg ccgggccatt gcggggctga atgtgaaggg gctgaagaaa
       61 aacgcgcggg atatcagtct gctgaggtat atgccggcct gacacaggct aagtgtgccc
      121 gggaatcggt gcaagtataa aaaacagcct gttgcggtat aagaatcgac catgcagaga
      181 gagaaaacac cggagtggag ggaaaagcag aaaagctcac gtggcataag gcgtggacag
      241 cggtatcgtc tggtttttca atttcctata cgttgaacgg gaaaattgcg caaatatcaa
      301 atttcatcaa taaatgaaaa tacacattga gtacagagat cactggggaa gaaagggttt
      361 ttgatgccgc gttagcggca tgtaaggcgg tactccgttg ttgcatatga cacaggagta
      421 aaaccgccga agcccggcgt aagccggtac ggattgatag atttcacctt acccaccccc
      481 agccctgcca gaccataccc gctttcagcc atgagagagc ttctgtgcgc ggtcggagtg
      541 gtcccgacga gggtttaccc gaagtcgggg cgtatctccg cgttagcggg ccgtgagggc
      601 cgcttacgag cgtgtactcg atacgtccag ccgtaagcgg ctcgccagaa ccgtattgat
      661 atttactgag a
//
LOCUS       DAKISA010000332          648 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.340, whole genome shotgun sequence.
ACCESSION   DAKISA010000332 DAKISA010000000
VERSION     DAKISA010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.340"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..194
                     /locus_tag="OVW45_004645"
     CDS             <1..194
                     /locus_tag="OVW45_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HCV5528888.1"
                     /translation="VDLHQVLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNL
                     HYIDTGAVFGGELTLVQLQ"
     gene            complement(195..386)
                     /locus_tag="OVW45_004646"
     CDS             complement(195..386)
                     /locus_tag="OVW45_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCV5528889.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(491..>648)
                     /locus_tag="OVW45_004647"
     CDS             complement(491..>648)
                     /locus_tag="OVW45_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCV5528890.1"
                     /translation="TIPCKSDPDLCMQLDAWDAETSIPALLNGEHSVLYRTRYDQQSD
                     AWIMRLA"
BASE COUNT          183 a          147 c          160 g          158 t
ORIGIN      
        1 acgttgattt gcatcaggtc ttgtggagcc gctcgcgatt aggtgaacgc caaaaagggc
       61 agggaattac aggtgctgat catttctggt ttggtcatac accgttgcga catcgcgtgg
      121 atattggcaa cctgcattat attgataccg gtgctgtctt tgggggcgaa ctgactcttg
      181 tgcaattgca ataattaaaa atcaccgtac tcctgtgcag gtcgccagaa accatctata
      241 aaatcctcaa tcggaaaaca accgccatgg cggatccgtt gatcgctcat agaataaaga
      301 cactgctgtt ccgtgttgta gacatccaca acaatatctt cacaaccgcc atccaggtag
      361 caaacaaaaa gtaccagcgc gaacatttca tccccgaagt gtggtgccgt accgttaagt
      421 ttaggagaga ttttacaacg ggggaataac caggacaaat aacccgccat cccgacgggt
      481 tctttttgga tcaggcaaga cgcataatcc aggcatcaga ttgttgatcg taacgggtac
      541 ggtaaaggac agagtgttcg ccatttagca gggcagggat actggtttca gcatcccagg
      601 catcgagttg catacataaa tcggggtcag atttacaagg aatggtta
//
LOCUS       DAKISA010000333          644 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.341, whole genome shotgun sequence.
ACCESSION   DAKISA010000333 DAKISA010000000
VERSION     DAKISA010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.341"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(<1..325)
                     /locus_tag="OVW45_004648"
     CDS             complement(<1..325)
                     /locus_tag="OVW45_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCV5528891.1"
                     /translation="MTKNTRFSPEVRQRAIRMVLESQDEYDSQWAAICSIAPKIGCTP
                     ETLRVWVRQHERDTGGGDGGLTTAERQRLKELERENRELRRSNDILRQASAYFAKAEF
                     DRLWKK"
     gene            379..582
                     /locus_tag="OVW45_004649"
                     /pseudo
     CDS             379..582
                     /locus_tag="OVW45_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414790.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          157 a          173 c          152 g          162 t
ORIGIN      
        1 attttttcca gaggcggtca aactccgcct tcgcaaaata agcggaagcc tggcgaagga
       61 tatcgttact gcggcgcagt tcacgatttt cacgctccag ctctttcaga cgctgacgtt
      121 cagcggtggt gagcccacca tcaccgcccc cggtatcccg ctcatgctgg cgaacccaga
      181 cacgcagagt ctccggcgta cagccaatct ttggggcaat ggaacaaatt gccgcccact
      241 gtgagtcata ttcatcctga ctttccagaa ccatacgaat cgcccgctga cggacttcgg
      301 gggaaaaacg agtattttta gtcatcctgt ttacctcttt ctcagggagt ttagtctcca
      361 ggatttccgg ggcggttcag acatctagaa tttactgtca gcaccggcgt taaagtttac
      421 ttctgcgatc ctcagagtcc ttggcagcgg ggaacaaatg agaatacaaa tgggctaatt
      481 cggcagtact ttcctaaaaa gacatgtctt gcccaatata ctcaacatcg agtaggcagc
      541 ctgacggctg cggcttgtca tggtctgcaa ttaccgttat aaaaacaggc aatgtcattg
      601 tctttcaggt ggttatatgg cccgttcagc taaaccccgt aaac
//
LOCUS       DAKISA010000334          644 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.342, whole genome shotgun sequence.
ACCESSION   DAKISA010000334 DAKISA010000000
VERSION     DAKISA010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.342"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..>644
                     /gene="fliD"
                     /locus_tag="OVW45_004650"
     CDS             <1..>644
                     /gene="fliD"
                     /locus_tag="OVW45_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCV5528892.1"
                     /translation="KVSTEAGAAPGIYTISVTQLAQAQSLRTDSPTIIASTKDALGDE
                     SSDTRTIKITQDGRKEPLEIKLNKDQTSLDEISKAINDADSGISASIVKVKDGNYQLV
                     LTASEGLANKMTISVEGDSKLNDLLAYDSKTNTGNMKELVNAQNAQLNVNGIDIERSS
                     NKITDAPQGVTLDLTKKVTDVRVTVTKSNDKATEAIKGWVDSYNSLIDTFNTLT"
BASE COUNT          219 a          146 c          139 g          140 t
ORIGIN      
        1 taaagtttcc accgaagcag gtgctgcccc cggtatctat accatcagcg taacccaact
       61 ggcgcaggcg caatctttac gaactgattc cccgacaatc attgcatcaa caaaagatgc
      121 actgggtgat gagtcatcag atacacgcac aataaaaatt acccaagacg gtcgtaaaga
      181 gccgctggaa atcaagctca ataaagatca gacgtcattg gatgagatca gcaaagccat
      241 taacgatgcc gatagtggaa tttccgctag tatcgttaaa gtcaaagatg gtaactatca
      301 attggtattg accgccagtg aaggtctggc caataaaatg accatatctg ttgagggtga
      361 cagtaaatta aatgatctgc tggcttatga cagcaagacc aacaccggga atatgaagga
      421 actggttaac gcgcaaaatg cacagctaaa cgttaacggt atcgatattg aacgttcaag
      481 taacaaaatt accgatgctc ctcagggagt gactctcgat ttgaccaaaa aagtcactga
      541 cgtccgggtc acggtgacaa aaagcaacga taaagcaacc gaagcaatta aaggctgggt
      601 ggattcatat aactccctca ttgacacctt taacaccctg acaa
//
LOCUS       DAKISA010000335          629 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.343, whole genome shotgun sequence.
ACCESSION   DAKISA010000335 DAKISA010000000
VERSION     DAKISA010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.343"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
BASE COUNT          222 a           98 c          127 g          182 t
ORIGIN      
        1 agtctatttt ataaaaaaat taagtaggct aagaagtacc ttagaaatca attttaaggg
       61 gtctaaaacg ctctgtaacg agctaaactg tataagtggc agtaagatgc cttttagact
      121 gacactcgct tagagagctt cctagagctt ctgggaggat tcattagata tcgttgtata
      181 cagagacaca gtatctataa agggtattaa gagattctta gaagttactg taaagaacca
      241 ctaagataag aagagaggga caaagaaccg aaggaaagtc ccttattaag taggagtctt
      301 aatagataac ttaaaagtct taaaggatat cttccaagaa gagaggaacg aagaaccaga
      361 ggaagtgacc gtttgaacag actgtttaag taaagcctat aaagtatcta ttataagggc
      421 tatgtctcaa tactagcagt ttgtcaatac ctttgtcttt agcaattcgt gctatttatt
      481 aaatatagca ctttttgtta aaacagagta tgctttttaa cctaaaacct tgtcgcttcg
      541 ctctgggtca catgttcttg ggttcttagt gagagttaca ctatcttcta aggtactcaa
      601 taaaaagacg agtaacaacc gaagaacga
//
LOCUS       DAKISA010000336          621 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.344, whole genome shotgun sequence.
ACCESSION   DAKISA010000336 DAKISA010000000
VERSION     DAKISA010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.344"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            39..>621
                     /locus_tag="OVW45_004651"
     CDS             39..>621
                     /locus_tag="OVW45_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HCV5528893.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHEISSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTPISHIRYSDILNYRNELLHGETLYLDNPRSNKKGRTVRT
                     VDNYIALLCSLLRFAYQSGFISTKPFEGVKKLQR"
BASE COUNT          225 a          110 c          130 g          156 t
ORIGIN      
        1 ctatccgaag ataaatcagt ttatcaaaga ctcatgatat gtctaaatta ccaacaggtg
       61 tcgagattcg aggtaaatac attcgcatct ggttcatgtt tcgaggaaaa cgatgtcggg
      121 aaacattgaa aggctgggag attactaaca gtaacattaa aaaggccggg aatttaagag
      181 cgttgatagt tcatgaaatc agttccggtg aatttgagta tttaagacgt tttccccagt
      241 ccagcactgg ggcaaaaatg gtgacaacga gggtcataaa aacattcggg gagctttgtg
      301 atatctggac aaaaattaaa gagacagagt taacaacaaa cacaatgaag aaaacgaaat
      361 cacaattaaa aacactcagg ataataattt gtgagagtac cccgatatcg catattcgtt
      421 atagcgatat cttaaactac cggaatgaac tgctgcatgg agaaacgctt tacctagata
      481 atccaagatc caacaaaaaa ggaagaaccg tgcgcacagt tgataactat atcgccctgc
      541 tctgttcgtt gttacgtttt gcgtatcagt cgggatttat atcaaccaaa ccatttgaag
      601 gagtaaaaaa attacagcga a
//
LOCUS       DAKISA010000337          605 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.345, whole genome shotgun sequence.
ACCESSION   DAKISA010000337 DAKISA010000000
VERSION     DAKISA010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.345"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..>605
                     /locus_tag="OVW45_004652"
     rRNA            <1..>605
                     /locus_tag="OVW45_004652"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          134 a          135 c          202 g          134 t
ORIGIN      
        1 tgtttgatgt tctaacgtgg acccgtgatc cgggttgcgg acagtgtctg gtgggtagtt
       61 tgactggggc ggtctcctcc taaagagtaa cggaggagca cgaaggttgg ctaatcctgg
      121 tcggacatca ggaggttagt gcaatggcat aagccagctt gactgcgagc gtgacggcgc
      181 gagcaggtgc gaaagcaggt catagtgatc cggtggttct gaatggaagg gccatcgctc
      241 aacggataaa aggtactccg gggataacag gctgataccg cccaagagtt catatcgacg
      301 gcggtgtttg gcacctcgat gtcggctcat cacatcctgg ggctgaagta ggtcccaagg
      361 gtatggctgt tcgccattta aagtggtacg cgagctgggt ttagaacgtc gtgagacagt
      421 tcggtcccta tctgccgtgg gcgctggaga actgaggggg gctgctccta gtacgagagg
      481 accggagtgg acgcatcact ggtgttcggg ttgtcatgcc aatggcactg cccggtagct
      541 aaatgcggaa gagataagtg ctgaaagcat ctaagcacga aacttgcccc gagatgagtt
      601 ctccc
//
LOCUS       DAKISA010000338          600 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.346, whole genome shotgun sequence.
ACCESSION   DAKISA010000338 DAKISA010000000
VERSION     DAKISA010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.346"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..>600
                     /locus_tag="OVW45_004653"
     CDS             <1..>600
                     /locus_tag="OVW45_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCV5528894.1"
                     /translation="AANDPTIKGGRLIVEKDGIAVFAAIEKGGLLEVKDGGLAFAVDQ
                     KAGGAIKTTTRVMEVFGTNRLGQFEIKNGIANNMLLENGGSLRVEENDFAYNTTVDSG
                     GLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYELDDGSGL
                     IVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGR"
BASE COUNT          179 a          101 c          177 g          143 t
ORIGIN      
        1 gcggcgaatg atcccacgat taaaggcggg cgcttaattg ttgaaaaaga tgggattgcc
       61 gtctttgccg ctatcgaaaa gggaggatta ctggaggtta aagacggggg attagcattt
      121 gcggtagatc agaaagcagg cggtgctatt aaaacaacca cgcgggtcat ggaggtattc
      181 ggaacaaacc gtctcggtca gttcgaaatc aagaatggta ttgctaacaa tatgctgttg
      241 gaaaacggcg gaagtttgcg agttgaagaa aatgacttcg cttataatac cactgtggat
      301 agtggcggct tactggaggt tatggatggc gggactgcaa ctggcgttga taaaaaagca
      361 ggcgggaaat taattgtctc aacgaatgcg ctggaagtga gtggtacaaa cagtaaaggc
      421 caatttagta taaaggatgg tgtgtcaaaa aattatgaac tggatgatgg ctccgggctc
      481 attgttatgg aggacactca ggccattgac actatccttg atgagcatgc cactatgcaa
      541 tcgctgggaa aggatactgg tacgaaagtg caggcaaatg ctgtatatga tctcggtcga
//
LOCUS       DAKISA010000339          595 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.347, whole genome shotgun sequence.
ACCESSION   DAKISA010000339 DAKISA010000000
VERSION     DAKISA010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.347"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(8..>595)
                     /locus_tag="OVW45_004654"
     CDS             complement(8..>595)
                     /locus_tag="OVW45_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl4 family transposase"
                     /protein_id="HCV5528895.1"
                     /translation="LAAQLCKLAPRLGKRLAADIAQALAEQTVVVPGTNAAAVVLPRL
                     ALQLITLRKQRDEVALEVEQRVLAHPLYPVLTSMPGVGVRTAARLLTEVACRAFASAA
                     HLAAYAGLAPVTRRSGSSIRGEHPSRRGNKALKRALFLSAFAALRDPLSRAYYTRKMS
                     QGKRHNQALIALARRRCDVLFAMMRDGTFYTPQGS"
BASE COUNT          121 a          163 c          197 g          114 t
ORIGIN      
        1 aagcatgtta tgacccctgc ggggtataaa aagtcccgtc gcgcatcatg gcgaacagaa
       61 cgtcgcagcg tcgtctcgcc agggcgataa gcgcctgatt gtgtcgtttt ccctgactca
      121 ttttgcgggt gtagtaagcc ctggagagcg gatccctgag cgcggcgaag gccgacagga
      181 acaacgcccg tttgagagct ttattacccc gtcgcgaggg atgctcaccg cgtattgacg
      241 agccggatcg ccgagttacc ggcgcaaggc cagcataagc agcgagatgt gcggcagagg
      301 cgaaggcgcg gcaggcgacc tcggtgagga gtctggctgc ggtcctgaca ccgactccgg
      361 gcatactggt caggaccggg taaagagggt gagcaagaac tcgctgttct acctcaagcg
      421 ccacctcgtc tctttgctta cgcagcgtga tgagctggag tgccagacgt ggcagtacta
      481 cggcagcggc attcgtgccg ggaacgacga cggtttgttc ggccagtgcc tgagctatgt
      541 ctgctgcaag gcgtttaccc agacgaggcg caagtttgca gagctgggct gccag
//
LOCUS       DAKISA010000340          580 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.348, whole genome shotgun sequence.
ACCESSION   DAKISA010000340 DAKISA010000000
VERSION     DAKISA010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.348"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..>580
                     /locus_tag="OVW45_004655"
     CDS             <1..>580
                     /locus_tag="OVW45_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCV5528896.1"
                     /translation="TCGCLNKHGQWKKLSFLKDAMRSRWMWNMRQLLLKAWSEGLAMP
                     ESLSHITTESQWRSLVLKAGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAH
                     YNGGASLSFRYLDHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGE
                     KLPQVYRALGMDKPEPVAKVCYAQMVKQFLSR"
BASE COUNT          148 a          127 c          194 g          111 t
ORIGIN      
        1 taacctgtgg atgtctgaat aagcatggtc agtggaaaaa gctgagcttc ctgaaagacg
       61 cgatgcgttc acggtggatg tggaatatgc ggcagctgct tctgaaagcg tggtcagagg
      121 ggctggcgat gccggagtcg ttgtcacata tcacgacgga atcacagtgg agaagcctgg
      181 tgctgaaagc cggaggaaaa tactggcatg tgtacatgtc gaaaaaaacg gccggcggga
      241 ggaatacggc gcgctacctg ggtcgttatc tgaagaagcc gccgatagcg gcctcccggc
      301 tggcacatta caacggaggg gcgagcctga gcttccgtta cctggaccac aaaacgggag
      361 aaacggcgac ggaaacgctg acacagcgtg agctggtcgc gaggctgaaa cagcacatcc
      421 cggagaagtt ttttaagatg gtgaggtact tcgggttcct tgccaaccgg gtgtgtggag
      481 agaagctgcc gcaggtgtac cgtgctctgg ggatggataa accggaacca gtggcgaaag
      541 tgtgctatgc acaaatggtg aaacagttcc tgagtcgtga
//
LOCUS       DAKISA010000341          576 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.349, whole genome shotgun sequence.
ACCESSION   DAKISA010000341 DAKISA010000000
VERSION     DAKISA010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.349"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..445
                     /locus_tag="OVW45_004656"
     CDS             <1..445
                     /locus_tag="OVW45_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HCV5528897.1"
                     /translation="IEDIEKQLTSVARQNETCKRLLDIPGVGPLIATAAVATMGEASA
                     FKSGREFAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTLFIHGARAVALVAKEPGPW
                     ITELKKRRPASVAIVAMANKLARTVWAITAHDRKYDRNHVSIRPY"
BASE COUNT          171 a          124 c          161 g          120 t
ORIGIN      
        1 gatagaggat attgaaaaac agcttaccag cgtggcgagg cagaatgaaa cctgtaagcg
       61 gttgctggat attcctggcg ttggaccact tattgcgacg gcagcggtgg ccaccatggg
      121 ggaagcatca gcgtttaaat cggggcgaga gttcgccgca tatgttggtc tggttccaaa
      181 acaaactggc tccggaggga aagtacgtct gctggggata agcaaacgtg gtgacactta
      241 tctcaggaca ttatttatcc acggtgcaag agcggtggca ttagtagcta aagagcctgg
      301 cccgtggata accgaactga aaaaacgtcg tccagccagt gtggcaatcg tcgccatggc
      361 aaacaagctg gcacgaacag tatgggcgat aaccgcccat gaccgtaagt atgacaggaa
      421 ccacgtcagt atcagaccat attaatcgct gataccatta aacaatgaac tcttaacaaa
      481 agggtgaatg ctgaaaggtt gctatggcgg ccagagtgat gacaaagaca ggtaagaccg
      541 tgactcacta aacctgaaca gtattttggg cttgaa
//
LOCUS       DAKISA010000342          566 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.350, whole genome shotgun sequence.
ACCESSION   DAKISA010000342 DAKISA010000000
VERSION     DAKISA010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.350"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            complement(1..526)
                     /locus_tag="OVW45_004657"
                     /pseudo
     CDS             complement(1..526)
                     /locus_tag="OVW45_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
BASE COUNT          153 a          156 c          140 g          117 t
ORIGIN      
        1 ctgcggcggt catattaata atagtattca tcagatcctg cacggtattg gaaccgagca
       61 tcgggataat catcagcggg atgacgatca ggaactggat ccacgctgcg cgcgccggaa
      121 cgccgttttc gttcagttcg acggttttct taccaaagat gccttccggg atttcggaga
      181 agaaaatttt caccggcgtt gcggtccaca tcagcaggga accgaacatc gcggtaaagg
      241 acaccagacc gacaaagcga ttcatcaacg cttccggtag accaaaatac gccgccatgc
      301 cgtggaatac ctgcaccgat ccgccggtaa atttcaactc tttgctgctg acgaagacgt
      361 taatcagcac cgaggagacg gaatacagta cgccgataaa aattccggcg aggatgatca
      421 ctttaacgaa cgatttcgaa ccgcctttga cgtcgttaac gtacacagcg acggactccg
      481 cgccacctgc ggccataaag atccaggtgg taacgccgag gaacgcctaa atagctgcgc
      541 ttaataccgc tacacttttg ccagcc
//
LOCUS       DAKISA010000343          560 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.351, whole genome shotgun sequence.
ACCESSION   DAKISA010000343 DAKISA010000000
VERSION     DAKISA010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.351"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            110..>560
                     /locus_tag="OVW45_004658"
     CDS             110..>560
                     /locus_tag="OVW45_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSso6 family transposase"
                     /protein_id="HCV5528898.1"
                     /translation="MNNNNTLYVGLDVHKESITVAYAINSEPVELMGKIGTSPTDIQN
                     LCKRLRSKSSQVSIVYEAGPCGYGLYRRLVKSGFDCMVCAPSLIPKKPGERVKTDRRD
                     AIRLVRSLRAGDLSAVYVPGIEDEAFRDLARAWASARDDLRHARQRLK"
BASE COUNT          135 a          132 c          149 g          144 t
ORIGIN      
        1 tgagacagat gctggccaac gtgggatatt taacgcgggt taacatcgtt gtcagagccg
       61 catgggtgag ctgttgctac accccggtat ttccaccacg gaggccagca tgaacaacaa
      121 taacacattg tacgtcgggt tagatgttca caaagaatcg attactgtcg cttatgctat
      181 caattcagaa cctgttgaac tgatgggtaa aattggcaca tcacctactg atattcagaa
      241 tctttgtaaa cgtctcaggt caaagtcatc gcaggtcagt atcgtttatg aagcggggcc
      301 ctgtggttat ggactttatc gtcggctggt gaaatccggt tttgactgca tggtctgcgc
      361 cccctcactt atcccgaaaa aaccggggga gcgggttaaa accgatcgcc gtgatgccat
      421 cagacttgtg cgttcactgc gtgcaggaga tctttctgct gtctacgtac ccggcattga
      481 agatgaggca ttccgggatt tggcccgggc atgggcatct gcccgcgatg atttacggca
      541 tgcaaggcaa cgtctgaaat
//
LOCUS       DAKISA010000344          559 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.352, whole genome shotgun sequence.
ACCESSION   DAKISA010000344 DAKISA010000000
VERSION     DAKISA010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.352"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..>559
                     /locus_tag="OVW45_004659"
     CDS             <1..>559
                     /locus_tag="OVW45_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCV5528899.1"
                     /translation="GELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLE
                     RKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAV
                     TTGGFNSETVQDVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPK
                     STAVRSPESNGIAESFVKTIKRDYIS"
BASE COUNT          134 a          132 c          182 g          111 t
ORIGIN      
        1 cggagagctg cccacgtatg gttatcgtcg ggtatgggcg ctgcttcgca gacaggcaga
       61 acttgatggt atgcctgcga tcaatgccaa acgtgtttac cggatcatgc gccagaatgc
      121 gctgttgctt gagcgaaaac ctgctgtacc gccatcgaaa cgggcacata caggcagagt
      181 ggctgtgaaa gaaagtaatc agcgatggtg ctctgacggg ttcgagttcc gctgtgataa
      241 cggagaaaaa ctgcgagtca cgttcgcgct ggactgctgt gaccgtgagg cactgcactg
      301 ggcggtcact acgggcggct tcaacagtga aacagtacag gacgtcatgc tgggagcggt
      361 ggaacgccgc ttcggcaacg agcttccggc gtctccagta gagtggctga cggataatgg
      421 ttcatgctac cgggctaatg aaacacggca gtttgcccgg atgttggggc ttgaaccgaa
      481 gagcacggcg gtgcggagtc cggagagtaa cggcatagca gagagcttcg tgaaaacgat
      541 aaagcgtgac tacatcagt
//
LOCUS       DAKISA010000345          556 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.353, whole genome shotgun sequence.
ACCESSION   DAKISA010000345 DAKISA010000000
VERSION     DAKISA010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.353"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..>556
                     /gene="feaR"
                     /locus_tag="OVW45_004660"
     CDS             <1..>556
                     /gene="feaR"
                     /locus_tag="OVW45_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HCV5528900.1"
                     /translation="WQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDAS
                     RPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMNN
                     PALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEWI
                     AGETGMSVRSLYRMFADKGLVVAQY"
BASE COUNT          142 a          125 c          146 g          143 t
ORIGIN      
        1 tggcaggaag taaaaggcag cgacgatgcc tggttttaca ccgtttttca gcttagtggt
       61 caggcaataa tggagcagga tgagcgtcag gtgcagattg gcgctggcga tattacgtta
      121 ctcgatgcct cacgcccctg ttcgctttac tggcaggagt cttctaaaca gatttcatta
      181 cttttgccac gcactctgct ggaacaatat tttccccatc aaaaacctgt ctgcgcagaa
      241 agactggacg ctgacttacc catggtgcaa ctcagtcatc gcctgttaca ggagagcatg
      301 aataatccgg cactttctga aacagaaagt gaagctgcgc tacaggcgat ggtgtgtctg
      361 ctgcgcccgg tacttcatca gcgggaatct gttcaacctc gccgtgaacg tcagtttcaa
      421 aaagtggtta cgttgataga cgataatatt cgcgaagaga tattacgccc ggagtggata
      481 gccggagaga caggtatgtc agtacgtagt ttgtaccgaa tgtttgccga taaaggtttg
      541 gtagtcgcgc aatata
//
LOCUS       DAKISA010000346          548 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.354, whole genome shotgun sequence.
ACCESSION   DAKISA010000346 DAKISA010000000
VERSION     DAKISA010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.354"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..516
                     /locus_tag="OVW45_004661"
     CDS             <1..516
                     /locus_tag="OVW45_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSso6 family transposase"
                     /protein_id="HCV5528901.1"
                     /translation="LESALKEAVTEWRLYPVVEALQAMRGIQFITAVGLISELGDLTR
                     FEHPRQLMSWFGITPSEYSSGGSRHQGSITKAGNSYARKLLVEAAWSYRHPARISPAI
                     QKRQENLPRPVIDRAWDAQLRLCKRYRKLQAKGKNVNITIVAVARELAGFIWDMGRIA
                     MSVAQQPQCHK"
BASE COUNT          159 a          122 c          144 g          123 t
ORIGIN      
        1 ctggaatccg ccctgaagga ggccgttacc gaatggaggc tgtatccggt agtagaggcg
       61 ctgcaagcca tgcgagggat tcagtttatt actgcggttg gattaatttc tgaactgggt
      121 gatctgaccc gttttgaaca tccacgacaa ctgatgtcat ggtttggtat cacgccatca
      181 gaatattcat cgggtggaag cagacatcag ggcagcatta ccaaagccgg aaacagctat
      241 gccagaaagc tattggtcga ggcagcgtgg agttaccgtc atccggcacg catcagtccg
      301 gcaatacaaa aaaggcagga aaatttaccc cgccccgtca ttgacagagc atgggatgct
      361 caactcaggc tttgtaagag gtatcgaaaa cttcaggcca aaggaaagaa tgtcaatatt
      421 acaattgttg ctgttgcacg tgagctggcg ggttttatct gggatatggg ccgaatagca
      481 atgtctgtcg cacaacaacc acaatgtcac aaataaaaaa tgagaataac cagttccctg
      541 aaggcgac
//
LOCUS       DAKISA010000347          546 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.355, whole genome shotgun sequence.
ACCESSION   DAKISA010000347 DAKISA010000000
VERSION     DAKISA010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.355"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            1..546
                     /locus_tag="OVW45_004662"
                     /pseudo
     CDS             1..546
                     /locus_tag="OVW45_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT          194 a          120 c           83 g          149 t
ORIGIN      
        1 ccgacttatt aaaaatgcct gtttaaaatt atataattta gcacataccg caaccaactc
       61 ccaccttaaa ccaactggtc cggataattc agatgttctc ctgttcaaaa agctattctc
      121 cccttcgaaa ttaatgacaa ttatcggtga tgaaattcct cttatatctg aaaaacagtc
      181 gctttcaaag gtgcttttaa atgatgagaa taatgaactg agtgatggta caaacttctg
      241 ggataaaaat cgtcaattaa ccacagatga aatagcttgc tatcttcaga agatcgccgc
      301 taatgcaaaa aatactcaag tcaattatcc tactggtctc tacgtcccct actccaccag
      361 aactcacctg gaagacgctc tcaatgaaaa tattaagagc gatccatcat ggccgaatga
      421 agtccagtta ttccccataa atactggcgg acactggata ttagtttcgc tacagaaaat
      481 agtaaataaa aaaataataa actacaaata aaatgcgtca tattcaactc attgcgtgca
      541 ctaggc
//
LOCUS       DAKISA010000348          529 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.denovo.356, whole genome shotgun sequence.
ACCESSION   DAKISA010000348 DAKISA010000000
VERSION     DAKISA010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.denovo.356"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            132..>529
                     /locus_tag="OVW45_004663"
     CDS             132..>529
                     /locus_tag="OVW45_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCV5528902.1"
                     /translation="MKYTPVGVDIAKHVIQIHFINEHTGEVVDKQLRRQDFLTFFGNR
                     EPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRMQLVKFRTA"
BASE COUNT          145 a          118 c          147 g          119 t
ORIGIN      
        1 cacacaatta aacacggagc agtatgatgg ttaaaagggt gaaactggca ggtgtggagc
       61 ctcgcagatt tccggcatca tagtgcccaa tacgggatgc ataaagctcc ctcagactgg
      121 agactccaca aatgaaatat acaccggttg gcgttgatat cgcaaaacat gtcattcaga
      181 ttcacttcat caatgagcac acaggtgaag tggttgataa acagttgcgt agacaggatt
      241 ttctgacgtt cttcggcaac cgtgagccat gcctgattgg tatggaggcc tgtggaggtt
      301 ctcagcactg ggcacgggaa ctgacaaaac ttggtcataa agtccggttg ttgcaggccc
      361 gcttcgttaa ggcattcgtc atgggcaata agaatgatgt gatggatgcc cgggctatct
      421 ggatggcggt tcagcagccg ggtaaagaaa tcgccgtaaa aacagaagaa cagcagtcgg
      481 tactggttct gcaccgtacc cgcatgcaac tggtgaagtt ccggaccgc
//
LOCUS       DAKISA010000349        10080 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.guided.139, whole genome shotgun sequence.
ACCESSION   DAKISA010000349 DAKISA010000000
VERSION     DAKISA010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10080
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.guided.139"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            1..652
                     /locus_tag="OVW45_004664"
                     /pseudo
     CDS             1..652
                     /locus_tag="OVW45_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            649..1263
                     /locus_tag="OVW45_004665"
     CDS             649..1263
                     /locus_tag="OVW45_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HCV5528903.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="OVW45_004666"
     CDS             complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="OVW45_004666"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCV5528904.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3039..3497
                     /gene="gpt"
                     /locus_tag="OVW45_004667"
     CDS             3039..3497
                     /gene="gpt"
                     /locus_tag="OVW45_004667"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCV5528905.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3589..4833
                     /gene="frsA"
                     /locus_tag="OVW45_004668"
     CDS             3589..4833
                     /gene="frsA"
                     /locus_tag="OVW45_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCV5528906.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4891..5292
                     /gene="crl"
                     /locus_tag="OVW45_004669"
     CDS             4891..5292
                     /gene="crl"
                     /locus_tag="OVW45_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCV5528907.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5331..6386)
                     /gene="phoE"
                     /locus_tag="OVW45_004670"
     CDS             complement(5331..6386)
                     /gene="phoE"
                     /locus_tag="OVW45_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCV5528908.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            6674..7777
                     /gene="proB"
                     /locus_tag="OVW45_004671"
     CDS             6674..7777
                     /gene="proB"
                     /locus_tag="OVW45_004671"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCV5528909.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7789..9042
                     /gene="proA"
                     /locus_tag="OVW45_004672"
     CDS             7789..9042
                     /gene="proA"
                     /locus_tag="OVW45_004672"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCV5528910.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9157..9232
                     /locus_tag="OVW45_004673"
     tRNA            9157..9232
                     /locus_tag="OVW45_004673"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9190..9192,aa:Thr,seq:cgt)
BASE COUNT         2522 a         2416 c         2618 g         2524 t
ORIGIN      
        1 acaccccatt ataaggggta cgaataaagt aacggggcag tctattttac agcggcatat
       61 tgcctcgttt tcgcatcatg gtttgcctga aggcagtgac gacgcgctac ggtatattgc
      121 ggaacatgac gatgcgctgg cgtttgcgcg tattaatcgc cagctgatct ctttacgcat
      181 aatgcaacag gttaaggcca ccggaagtcc ggttctggat gtggcgcata actttgttag
      241 cgcgtgtcaa atcggcgatc aacagggctg gttgcatcgt aaaggtgcca cgccggatga
      301 caacggtctg gtgattattc ccggttcact cggtgattac tcctagcttg tacagcctgt
      361 tgcgaacgaa aaaacgctcc actcgctggc gcatggggct gggcgtaaat gggggcgcac
      421 cgagtgtaaa gggcgtctgg cagcgaaata cacagcgacg cagctctcac gtactgaact
      481 tggcagtcgg gtaatttgtc gcgataaaca actcatcttt gaagaagcgc cacaagccta
      541 taaatcggct gaaagcgtgg tgcaatgtct ggtgcaggct ggtttaatta ttcctgtcgc
      601 gcggctgcgt ccggtgctaa cgctcaaaaa cagtggaggg aaaaaaggat gatcttgcta
      661 caactctcct cagcgcaggg gccggaagaa tgttgtctcg cagtgaaaaa ggcgctggag
      721 cgattaatta aagaagctgc ccgacaagac gtcgcggtaa cggtgttgga aacagaaacg
      781 ggccgctact ctgacacgct gcgttcagct ctggtttctc tggatggcga taacgcatgg
      841 gcattaagcg aaagctggtg cggcactatt cagtggattt gtccgagtcc gtatcggcct
      901 catcatgggc gcaaaaactg gtttctgggc attgggcgtt ttaccgctga tgagcaggaa
      961 caatcggatg caatccgtta tgagacgttg cgttcgtcgg ggccgggcgg tcaacatgtc
     1021 aataaaaccg actcggcggt acgcgccacg catttggcat ccggtattag cgtgaaggtt
     1081 cagtcagagc gtagtcagca tgctaacaag cggctggcac gattgctgat tgcctggaag
     1141 ctggagcagc agcaacagga aaatagcgcg gtgctgaaat cgcagcggcg aatgttccat
     1201 caccagattg aacgtggcaa cccgagacgg acatttacag ggatggcttt tatcgaagga
     1261 taatgaagga tgaaactgcc ggaaggcgat taaacgccat ccggcagcaa atcaaataat
     1321 tacttcgccg gaatttcttt cagcagttca gtcagcagtg tccagtaatg acctacgctt
     1381 tcgatgtgaa cttgctcatc cggagagtgt ggaccggtga tagttggccc gatagaaacc
     1441 atgtccattt ccggatacgg ttttttgaac agaccacatt ccaggcccgc gtggataatc
     1501 tggatgttcg gcgttttgtt gaacagacgc tgataggttt cacgtaccag atgcatcacc
     1561 ggagaattag cgtccggctg ccagccagga tatgcgcctt tcgcttcggt tttcgcgcca
     1621 gccagtttac ccagcgaatc cagcatgctc accacgtagt ctttaccgct gtcgatcaga
     1681 gaacggatca gacagtgaat ttctacgtta ttatcagtca tggtcaccac accgacgttc
     1741 agggaggttt cgaccacacc ttttgccacg tctgagttgc ggatcacacc gttcggggtg
     1801 gcgttcagca gacgaataaa ggtatcgcga gatttcgcaa tcagggcagc tttatcgttc
     1861 gctacagagt ccagcaacaa ggccagattt ttctctttct ctgccagctc gtttttcagg
     1921 atctcctgat aggtattcac cagagatttc aggacgtcga ctttatcagc tgcgacagca
     1981 atggtcgcaa aggcttcacg cgggatggcg ttacgcagtg tgccgccgtt gaaatcgata
     2041 aggcgcagat ccagttcttc tgcatgaccc gctaggaagc gcaccagcag tttgttggca
     2101 ttacccaggc caacgtggat ttccccgccg gagtgacccc ctttcagacc ttttaaggtt
     2161 aacttgaagg tttcaaaacc agctggaacc gcttcacgat ctaaatgcag gttggaggtg
     2221 aagtcgatac cccccgcaca acccatgtag atttcacctt cttcttcgga gtcggtgtta
     2281 atcagaatat cagcctgcaa ccagttgctc tgtaagccga acgcaccgtc cataccggct
     2341 tcttcggtca tggtcagcag cacttccagc gggccgtgaa ccacgttttc gtcagccaga
     2401 accgccagcg cagacgccat accaatgccg ttatctgcac ccagcgtggt gccgcgcgct
     2461 ttaacccatt cgccatcaat ataaggctgg ataggatctt tcgtgaagtc atgcacggtg
     2521 tcgttatttt tctgcggcac catatcgagg tgggcctgta agacgaccga tttacgattt
     2581 tccatacctg cggtagcagg tttacgaatc aggatattac ctacctgatc gcgttcgaca
     2641 tggaaacctt tctcttttgc ccaaccaaca atgtattcag cgagttgctc ttcatgatag
     2701 gacgggtgag gaatagaaca gattttggca aaaatatccc acagcggctg tggagataat
     2761 tgagacagtt cagacacgtt aagtctcctt gtcgatcacc cgcaaaacag tattgcaggt
     2821 cacagggtta gcagaaaatg ttgtcaacac aagacaggct tgcgagatat gtttgagaat
     2881 accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat
     2941 actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc ccctcgaaca
     3001 ctttttaagc cgtagataaa caggctggga cacttcacat gagcgaaaaa tacatcgtca
     3061 cctgggacat gttgcagatc catgcacgta aactcgcaag ccgactgatg ccttctgaac
     3121 aatggaaagg cattattgcc gtaagccgtg gcggtctggt accgggtgcg ttactggcgc
     3181 gtgaactggg tattcgtcat gtcgataccg tttgtatttc cagctacgat cacgacaacc
     3241 agcgcgagct taaagtgcta aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg
     3301 atgacctggt ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat ccaaaagcgc
     3361 actttgtcac catcttcgca aaaccggctg gtcgtccgct ggttgatgac tatgttgttg
     3421 atatcccgca agatacctgg attgaacagc cgtgggatat gggcgtcgta ttcgtcccgc
     3481 caatctccgg tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt ctttttaact
     3541 tcaggcgggt tacaatagtt tccagtaagt attctggagg ctgcatccat gacacaggca
     3601 aacctgagcg aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc gacgctagtc
     3661 cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg cccttgatgg taaaaccatc
     3721 cctcactggt atcgcatgat taaccgtctg atgtggatct ggcgcggcat tgacccacgt
     3781 gaaatcctcg acgtccaggc gcgtattgtg atgagcgatg ccgaacgtac cgacgatgat
     3841 ttatacgata cggtgattgg ctaccgtggt ggcaactgga tttatgagtg ggccatccag
     3901 gcgatggtgt ggcaacaaaa agcctgtgcg gaagaagatc cgcaactcag tggtcgtcac
     3961 tggctgcatg cggctacgtt gtacaacatt gctgcctatc ctcatctgaa aggagatgac
     4021 ctggccgagc aagcgcaggc tttgtcaaac cgtgcctatg aagaagccgc ccagcgtctg
     4081 ccgggcacga tgcggcagat ggagtttacc gtacccggcg gtgcgcccat caccggcttt
     4141 ttgcatatgc cgaaaggcga tggcccgttc ccgacagtat taatgtgtgg tggtctggat
     4201 gcgatgcaga cggactatta cagcctgtat gaacgttatt ttgcgccgcg cggcattgcg
     4261 atgctgacta ttgatatgcc gtcggtgggc ttttcatcaa aatggaagct cacccaggac
     4321 tccagcctgt tgcatcagca cgttttaaag gctctgccta acgtaccgtg ggtggatcac
     4381 actcgcgtcg cggcctttgg tttccgtttc ggcgctaacg ttgccgtgcg tctggcatat
     4441 cttgaatcgc cgcgtctgaa agcggttgcc tgtcttggtc cggtagttca taccctgttg
     4501 agtgatttta agtgccagca acaggtgccg gaaatgtatc ttgacgttct ggcgagtcgt
     4561 ttggggatgc atgatgcttc cgacgaagcg ttacgcgtgg agctgaatcg ctattcatta
     4621 aaagtgcaag gattgctggg gcgtcgctgc ccaacgccaa tgttatcagg ctactggaag
     4681 aacgatccgt tcagcccgga agaggactca cgcttaatca cctcatcatc tgctgacggt
     4741 aaattattag agatcccatt taacccggtg tatcggaatt ttgacaaagg tcttcaggaa
     4801 atcaccggct ggatcgaaaa acgcttgtgt taaaaatttg ctaaaatttg ccaatttggt
     4861 aaaacagttg catcacaaca ggagattgca atgacgttac cgagtggaca cccgaagagc
     4921 agattgatca aaaaatttac cgcactaggc ccgtatattc gtgaaggtaa gtgcgaagat
     4981 aatcgattct ttttcgattg tctggctgta tgcgtcaacg tgaaaccggc accggaagtg
     5041 cgtgagttct ggggctggtg gatggagctt gaagcgcagg aatcccgttt tacctacagt
     5101 taccagtttg gtctgttcga taaagcaggc gactggaaga gtgttccggt aaaagacact
     5161 gaagtggttg aacgactgga gcacacccta cgtgagtttc acgagaagct gcgtgaactg
     5221 ctgacgacgc tgaatctgaa gctggaaccg gcggatgatt ttcgtgatga accggtgaag
     5281 ttaacggcgt gagtgaaatg tgccggatgt atcacatccg gcaatatcca ttaaaactga
     5341 taggtcatgc caaccgcgac aatatcatca ttattaatat tcaatttgtt atcgctatcc
     5401 agttggttga ttttataatc aacaaacgct gacatatttt tgttgaaata atacgtagca
     5461 ccgacgtcga tataattgac cagatcttca tcaccgatac cttcaatatc tttccctttc
     5521 gataagacat aacccagcga tggacgcaga ccaaagtcaa actggtattg agcgaccgct
     5581 tcaaagttct gtgtcttatt ggcaaagccg ccagttattg gcgtcatttt gcgtgtttca
     5641 gaatagaaag ttgccagata aatattattg gcatcgtatt tcagacctgt tgcccatgct
     5701 tctgcacgct tgcctgtgcc acggctttgc aggttctgct cgttggtgcg atctgagttg
     5761 gtataggccc cactaatggc gaaatcgctg ccgccaaagt catatgtcaa tgacgtgccg
     5821 aagccatcgc cgttttgctt tttaacgtcg cggttttcgt ttttcccttg atattgcagg
     5881 gttaagttca ggccatcgat aacgccgaag aagtcggtgt tccgatacgt cgccagaccg
     5941 ctggcgcgtt tggtcataaa gttgtcggtc tgcgccgagg aatcgccacc aaattccggg
     6001 aacatatcgg tccaggcttc cacgtcatac aacgcgccca ggttacgacc ataatcgaaa
     6061 gaacccaaat ctttatattt caacccggca aaagcgagac gcgttttctg ctgtgcagta
     6121 tcactctccg ctttatttcc ggcaaactcc gcttcccacc gaccataacc agtcagttga
     6181 tcgttaattt gtgtttcacc tttaaaacca aaacggatat aactctggtc gccatcttta
     6241 ctggcgttat cactcatata atgcatggct ttaactttgc catagacatc cagtttatta
     6301 ccgtctttat tatatatttc tgcagcctgt acagatgcag atgccacaat gcccatcacc
     6361 actaatgcca gagtgctctt tttcattttc attcctgatt ttaattaacg cgcgaatatt
     6421 cagcgggaga gccccgttga aaacaggaaa gtttttaacc tgagattgtt aaagatatat
     6481 tacagattaa taatattctt aaaatgtggt aatttattaa atctgtaata aaagcgtaaa
     6541 caactgccgc taggcttgct gatcccgcgc aacaaaacgc catgctttgc tcgcagatgg
     6601 ttggcaaccg acgacagtcc tgctaaaacg ttcgtttgat atcatttttc ctaaaattga
     6661 atggcagaga atcatgagtg acagccagac gctggtggta aaactcggca ccagtgtgct
     6721 aacaggcgga tcgcgccgtc tgaaccgtgc ccatattgtt gaacttgttc gccagtgcgc
     6781 gcagttacat gccgccgggc atcggattgt tattgtgacg tcgggcgcga tcgccgccgg
     6841 acgtgagcac ctgggttacc cggaactgcc agcgactatc gcctcgaaac aactgctggc
     6901 agcggtaggg cagagtcgac tgattcaact gtgggaacag ctgttttcga tttatggcat
     6961 tcacgtcggg caaatgctgc tgacccgtgc tgatatggaa gaccgtgaac gcttcctgaa
     7021 cgcccgcgac accctgcgtg cgttgctcga taacaatatc gttccggtaa tcaatgagaa
     7081 cgatgctgtc gctacggcag agattaaagt cggcgataac gataaccttt ctgcgctggc
     7141 ggcgattctg gcgggtgccg ataaactgtt gctgctgacc gatcaaaaag gtttgtatac
     7201 cgctgacccg cgcagcaatc cgcaggcaga actgattaaa gatgtttacg gcattgatga
     7261 cgcactgcgc gcgattgccg gtgacagcgt ttcaggcctc ggaactggcg gcatgagtac
     7321 caaattgcag gccgctgacg tggcttgccg tgcgggtatc gacaccatta ttgccgcggg
     7381 cagcaagccg ggcgttattg gtgatgtgat ggaaggcatt tccgtcggta cgctgttcca
     7441 tgcccaggcg actccgcttg aaaaccgtaa acgctggatt ttcggtgcgc cgccggcggg
     7501 tgaaatcacg gtagatgaag gggcaactgc cgccattctg gaacgcggca gctccctgtt
     7561 gccgaaaggc attaaaagcg tgactggcaa tttctcgcgt ggtgaagtca tccgcatttg
     7621 caacctcgaa ggccgcgata tcgcccacgg cgtcagtcgt tacaacagcg atgcattacg
     7681 ccgtattgcc ggacaccact cgcaagaaat tgatgcaata ctgggatatg aatacggccc
     7741 ggttgccgtt caccgtgatg acatgattac ccgttaagga gctggccgat gctggaacaa
     7801 atgggcattg ctgcgaagca agcctcgtat aaattagcgc aactctccag ccgcgaaaaa
     7861 aatcgcgtgc tggaaaaaat cgccgatgaa ctggaagcac aaagcgaaat catcctcaac
     7921 gctaacgccc aggatgttgc tgacgcgcga gccaatggcc ttagcgaagc gatgcttgac
     7981 cgtctggcac tgacgcccgc acggctgaaa ggcattgccg acgatgtacg tcaggtgtgc
     8041 aacctcgccg atccggtggg gcaggtaatc gatggcggcg tactggacag cggcctgcgt
     8101 cttgagcgtc gtcgcgtacc gctgggggtt attggcgtga tttatgaagc gcgcccgaac
     8161 gtgacggttg atgtcgcttc gctgtgcctg aaaaccggta atgcggtgat cctgcgcggt
     8221 ggcaaagaaa cgtgtcgcac taacgctgca acggtggcgg tgattcagga cgccctgaaa
     8281 tcctgcggct taccggcggg tgccgtgcag gcgattgata atcctgaccg tgcgctggtc
     8341 agtgaaatgc tgcgtatgga taaatacatc gacatgctga tcccgcgtgg tggcgctggt
     8401 ttgcataaac tgtgccgtga acagtcgaca atcccggtga tcacaggtgg tataggcgta
     8461 tgccatattt acgttgatga aagtgtagag atcgctgaag cattaaaagt gatcgtcaac
     8521 gcgaaaactc agcgtccgag cacatgtaat acggttgaaa cgttgctggt gaataaaaac
     8581 atcgccgata gcttcctgcc cgcattaagc aaacaaatgg cggaaagcgg cgtgacatta
     8641 cacgcagatg cagctgcact ggcgcagttg caggcaggcc ctgcgaaggt ggttgctgtt
     8701 aaagccgaag agtatgacga tgagtttctg tcattagatt tgaacgtcaa aatcgtcagc
     8761 gatcttgacg atgccatcgc ccatattcgt gaacacggca cacaacactc cgatgcgatc
     8821 ctgacccgcg atatgcgcaa cgcccagcgt tttgttaacg aagtggattc gtccgctgtt
     8881 tacgttaacg cctctacgcg ttttaccgac ggcggccagt ttggtctggg tgcggaagtg
     8941 gcggtaagca cacaaaaact ccacgcgcgt ggcccaatgg ggctggaagc actgaccact
     9001 tacaagtgga tcggcattgg tgattacacc attcgtgcgt aaataaaacc gggtgatgca
     9061 aaagtagcca tttgattcac aaggccattg acgcatcgcc cggttagttt taaccttgtc
     9121 caccgtgatt cacgttcgtg aacatgtcct ttcagggccg atatagctca gttggtagag
     9181 cagcgcattc gtaatgcgaa ggtcgtaggt tcgactccta ttatcggcac cattaagaaa
     9241 attaataact tagcttaact tctctaagaa attgtatctt ctgttatcac ttacaaacgc
     9301 ctatacgttg ttatgggctt tggtagtgta acacgtatgg gcgagtagtt cccaggctca
     9361 acctcgtaac aagttccttt gaagctggaa gacgtgccac cgtctctaaa ccttctacca
     9421 aaactcttag caagacatat aacgccttga attgatgtat caggggaaaa gtgatggatt
     9481 ttttacgtag gagcacgaaa gggcttctat tgacttttgt atgctatgaa atataattaa
     9541 agttttttgt ttttattgtg tgatgtggtg ggttacacag gggaatagat taaatggtgt
     9601 tctgtttata caggtttatt tgttagatca acaatttggc atttctaaac agatgtttga
     9661 acgttgccaa atgtcttgac aacacaagaa aagggcttgc tatgccaggc attacacgaa
     9721 ataaggctat aaaaacaaaa taaattttaa aatttgcaca aacgagaaac tcgttcagaa
     9781 acaagcagaa caatttttag ccaacaattt ctgatggtgc tctgtaagcc gcattacaca
     9841 aggctttgcg aaagtgatgt aaaagtgaag aaagtaagaa ttgaactttt aagctacttt
     9901 tcattggatt ttttagtagg gaattacaca gggaatagag agcattattc tatgatttac
     9961 acagggaata tagggggatt atggcacaca catagaaaga cattacacag agtgctatgt
    10021 gacaaatgga tgtaacgaga ggtgaggtgt actggcaata gcggacacta ccatttgttc
//
LOCUS       DAKISA010000350         8439 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.guided.151, whole genome shotgun sequence.
ACCESSION   DAKISA010000350 DAKISA010000000
VERSION     DAKISA010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8439
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.guided.151"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            996..1811
                     /gene="sul2"
                     /locus_tag="OVW45_004674"
     CDS             996..1811
                     /gene="sul2"
                     /locus_tag="OVW45_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCV5528911.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1872..2675
                     /locus_tag="OVW45_004675"
                     /note="aph(3'')-Ib"
     CDS             1872..2675
                     /locus_tag="OVW45_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCV5528912.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2675..3511
                     /locus_tag="OVW45_004676"
     CDS             2675..3511
                     /locus_tag="OVW45_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCV5528913.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            3817..4059
                     /locus_tag="OVW45_004677"
     CDS             3817..4059
                     /locus_tag="OVW45_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HCV5528914.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(4091..4741)
                     /gene="tetR(A)"
                     /locus_tag="OVW45_004678"
     CDS             complement(4091..4741)
                     /gene="tetR(A)"
                     /locus_tag="OVW45_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HCV5528915.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            4847..6046
                     /gene="tet(A)"
                     /locus_tag="OVW45_004679"
     CDS             4847..6046
                     /gene="tet(A)"
                     /locus_tag="OVW45_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HCV5528916.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWRN
                     SSNSRCT"
     gene            complement(6013..6201)
                     /locus_tag="OVW45_004680"
                     /pseudo
     CDS             complement(6013..6201)
                     /locus_tag="OVW45_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(6245..6634)
                     /locus_tag="OVW45_004681"
     CDS             complement(6245..6634)
                     /locus_tag="OVW45_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528917.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            6852..7292
                     /locus_tag="OVW45_004682"
     CDS             6852..7292
                     /locus_tag="OVW45_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCV5528918.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            complement(8059..8208)
                     /locus_tag="OVW45_004683"
     CDS             complement(8059..8208)
                     /locus_tag="OVW45_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528919.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
BASE COUNT         1859 a         2349 c         2330 g         1901 t
ORIGIN      
        1 ccgcttcctc gctcactgac tcgctacgct cggtcgttcg gctgcggcga gcggtaccgg
       61 cttacggacg gggacggaaa tttcctggaa gataccagga agacacttag caaagaagag
      121 ataaggccac ggcgaagccg tttttccata ggctccgccc ccctgacaag catcgcaaaa
      181 tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatcc caggcgtttc
      241 cccctggtag ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc
      301 cgctgttatg gccgcggttt ctcattccac gcctgacact cagttccgtg aaggcagttc
      361 gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttgtccg
      421 gtaactatca acttgagtcc aacccggaaa gacacgacaa aacgccactg gcagcagcca
      481 ttagtaacag gagtgcacga gaagatagat taagaggtct tgaagtagag gcctgactgc
      541 ggctacactg aaagaacagt tttggtaatg gcggtctcgt gaaccctgtt accgggttaa
      601 gcagttcccc aactgactta accttcgaac aaaccgcctc cccaggcggt tttttcgttt
      661 acagagcaag agattacgat tcattgaaaa ggatctcaag aagatccttt gtagattact
      721 atcaattaat cttccgaaca tgacaccagt cactgttgat tacggttaaa ccgctccgta
      781 acaggtttcc ctcgtattgg tgacttgcag caccaaagaa gcaaaaaaga taaatatatc
      841 tacttattga gagatgatat atttatttcc aaaatgctac agatacatca tcatgaaacg
      901 gatcaccgca aacaggttac tcgcctgggg attccctttc gacccgagca tccgtatgag
      961 actcatgctc gattattatt attatagaag cccccatgaa taaatcgctc atcattttcg
     1021 gcatcgtcaa cataacctcg gacagtttct ccgatggagg ccggtatctg gcgccagacg
     1081 cagccattgc gcaggcgcgt aagctgatgg ccgagggggc agatgtgatc gacctcggtc
     1141 cggcatccag caatcccgac gccgcgcctg tttcgtccga cacagaaatc gcgcgtatcg
     1201 cgccggtgct ggacgcgctc aaggcagatg gcattcccgt ctcgctcgac agttatcaac
     1261 ccgcgacgca agcctatgcc ttgtcgcgtg gtgtggccta tctcaatgat attcgcggtt
     1321 ttccagacgc tgcgttctat ccgcaattgg cgaaatcatc tgccaaactc gtcgttatgc
     1381 attcggtgca agacgggcag gcagatcggc gcgaggcacc cgctggcgac atcatggatc
     1441 acattgcggc gttctttgac gcgcgcatcg cggcgctgac gggtgccggt atcaaacgca
     1501 accgccttgt ccttgatccc ggcatggggt tttttctggg ggctgctccc gaaacctcgc
     1561 tctcggtgct ggcgcggttc gatgaattgc ggctgcgctt cgatttgccg gtgcttctgt
     1621 ctgtttcgcg caaatccttt ctgcgcgcgc tcacaggccg tggtccgggg gatgtcgggg
     1681 ccgcgacact cgctgcagag cttgccgccg ccgcaggtgg agctgacttc atccgcacac
     1741 acgagccgcg ccccttgcgc gacgggctgg cggtattggc ggcgctgaaa gaaaccgcaa
     1801 gaattcgtta actgcacatt cgggatattt ctctatattc gcgcttcatc agaaaactga
     1861 aggaacctcc attgaatcga actaatattt tttttggtga atcgcattct gactggttgc
     1921 ctgtcagagg cggagaatct ggtgattttg tttttcgacg tggtgacggg catgccttcg
     1981 cgaaaatcgc acctgcttcc cgccgcggtg agctcgctgg agagcgtgac cgcctcattt
     2041 ggctcaaagg tcgaggtgtg gcttgccccg aggtcatcaa ctggcaggag gaacaggagg
     2101 gtgcatgctt ggtgataacg gcaattccgg gagtaccggc ggctgatctg tctggagcgg
     2161 atttgctcaa agcgtggccg tcaatggggc agcaacttgg cgctgttcac agcctatcgg
     2221 ttgatcaatg tccgtttgag cgcaggctgt cgcgaatgtt cggacgcgcc gttgatgtgg
     2281 tgtcccgcaa tgccgtcaat cccgacttct taccggacga ggacaagagt acgccgcagc
     2341 tcgatctttt ggctcgtgtc gaacgagagc taccggtgcg gctcgaccaa gagcgcaccg
     2401 atatggttgt ttgccatggt gatccctgca tgccgaactt catggtggac cctaaaactc
     2461 ttcaatgcac gggtctgatc gaccttgggc ggctcggaac agcagatcgc tatgccgatt
     2521 tggcactcat gattgctaac gccgaagaga actgggcagc gccagatgaa gcagagcgcg
     2581 ccttcgctgt cctattcaat gtattgggga tcgaagcccc cgaccgcgaa cgccttgcct
     2641 tctatctgcg attggaccct ctgacttggg gttgatgttc atgccgcctg tttttcctgc
     2701 tcattggcac gtttcgcaac ctgttctcat tgcggacacc ttttccagcc tcgtttggaa
     2761 agtttcattg ccagacggga ctcctgcaat cgtcaaggga ttgaaaccta tagaagacat
     2821 tgctgatgaa ctgcgcgggg ccgactatct ggtatggcgc aatgggaggg gagcagtccg
     2881 gttgctcggt cgtgagaaca atctgatgtt gctcgaatat gccggggagc gaatgctctc
     2941 tcacatcgtt gccgagcacg gcgactacca ggcgaccgaa attgcagcgg aactaatggc
     3001 gaagctgtat gccgcatctg aggaacccct gccttctgcc cttctcccga tccgggatcg
     3061 ctttgcagct ttgtttcagc gggcgcgcga tgatcaaaac gcaggttgtc aaactgacta
     3121 cgtccacgcg gcgattatag ccgatcaaat gatgagcaat gcctcggaac tgcgtgggct
     3181 acatggcgat ctgcatcatg aaaacatcat gttctccagt cgcggctggc tggtgataga
     3241 tcccgtcggt ctggtcggtg aagtgggctt tggcgccgcc aatatgttct acgatccggc
     3301 tgacagagac gacctttgtc tcgatcctag acgcattgca cagatggcgg acgcattctc
     3361 tcgtgcgctg gacgtcgatc cgcgtcgcct gctcgaccag gcgtacgctt atgggtgcct
     3421 ttccgcagct tggaacgcgg atggagaaga ggagcaacgc gatctagcta tcgcggccgc
     3481 gatcaagcag gtgcgacaga cgtcatacta gatatcaagc gacttctcct atcccctggg
     3541 aacacatcaa tctcaccgga gaatatcgct ggccaaagcc ttagcgtagg attccgcccc
     3601 ttcccgcaaa cgacccctgg aagccccgta gcgacgcgga gaggggcgag acaagccaag
     3661 ggcgcaggct cgatgcgcag cacgacatag ccggttctcg caaggacgag aatttccctg
     3721 cggtgcccct caagtgtcaa tgaaagtttc caacgcgagc cattcgcgag agccttgagt
     3781 ccacgctaga tctatctcat ctgcgcaagg cagaacgtga agacggccgc cctggacctc
     3841 gcccgcgagc gccaggcgca cgaggccggc gcgcggaccc gcgccacggc ccacgagcgg
     3901 acgccgcagc aggagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg
     3961 acgctagggc agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg
     4021 aaagggggag gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca
     4081 gcggtcctga tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc
     4141 ttttcgccaa tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg
     4201 cttcgtcgaa ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg
     4261 tgccgccgcg ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag
     4321 tgaagtagct gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc
     4381 agaggaagcg aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg
     4441 catggatgcg cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc
     4501 cgatcagaaa tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg
     4561 ccagcatggc ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa
     4621 gcgccggctg ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc
     4681 cgacctcgtt caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca
     4741 tgcttgacac tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa
     4801 tccgcgcgtt caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacag
     4861 acccctgatc gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat
     4921 gccggtgctg ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta
     4981 tggcattctg ctggcgctgt atgcgttgat gcaatttgcc tgcgcacctg tgctgggcgc
     5041 gctgtcggat cgtttcgggc ggcggccggt cttgctcgtc tcgctggccg gcgctgctgt
     5101 cgactacgcc atcatggcga cggcgccttt cctttgggtt ctctatatcg ggcggatcgt
     5161 ggccggcatc accggggcga ctggggcggt agccggcgct tatattgccg atatcactga
     5221 tggcgatgag cgcgcgcggc acttcggctt catgagcgcc tgtttcgggt tcgggatggt
     5281 cgcgggacct gtgctcggtg ggctgatggg cggtttctcc ccccacgctc cgttcttcgc
     5341 cgcggcagcc ttgaacggcc tcaatttcct gacgggctgt ttccttttgc cggagtcgca
     5401 caaaggcgaa cgccggccgt tacgccggga ggctctcaac ccgctcgctt cgttccggtg
     5461 ggcccggggc atgaccgtcg tcgccgccct gatggcggtc ttcttcatca tgcaacttgt
     5521 cggacaggtg ccggccgcgc tttgggtcat tttcggcgag gatcgctttc actgggacgc
     5581 gaccacgatc ggcatttcgc ttgccgcatt tggcattctg cattcactcg cccaggcaat
     5641 gatcaccggc cctgtagccg cccggctcgg cgaaaggcgg gcactcatgc tcggaatgat
     5701 tgccgacggc acaggctaca tcctgcttgc cttcgcgaca cggggatgga tggcgttccc
     5761 gatcatggtc ctgcttgctt cgggtggcat cggaatgccg gcgctgcaag caatgttgtc
     5821 caggcaggtg gatgaggaac gtcaggggca gctgcaaggc tcactggcgg cgctcaccag
     5881 cctgacctcg atcgtcggac ccctcctctt cacggcgatc tatgcggctt ctataacaac
     5941 gtggaacggg tgggcatgga ttgcaggcgc tgccctctac ttgctctgcc tgccggcgct
     6001 gcgtcgcggg ctttggagaa attcttcaaa ttcccgttgc acatagcccg gcaattcctt
     6061 tccctgctct gccataagcg cagcgaatgc cgggtaatac tcgtcaacga tctgatagag
     6121 aagggtttgc tcgggtcggt ggctctggta acgaccagta tcccgatccc ggctggccgt
     6181 cctggccgcc acatgaggca tgttccgcgt ccttgcaata ctgtgtttac atacagtcta
     6241 tcgcttagcg gaaagttctt ttaccctcag ccgaaatgcc tgccgttgct agacattgcc
     6301 agccagtgcc cgtcactccc actcttacat tgttcgcagt atctaggtac atgttattgc
     6361 gtgcaccttc atgatgtata atgttattaa tgacagcaga aagatcgaca gaattaatat
     6421 tcttcgtacc tgggacagtt atcgggtgtc ggccagtgat gagaccaaag gatgcagtat
     6481 aagatgaacc actactatta tcatggccac cattaccacc accgttaaaa ctacctcccg
     6541 aacttccaga gccgctatga ccatcattgt aggggtcatt tggtcccatg tgaccaaaac
     6601 caccgacagt accgctattt aactctgtac tcataaaatt atcctctttg acttttaaaa
     6661 acagcaaaaa ctgtatttat aacaatgtgt ttatatacag tattcgaaat actacgcccg
     6721 tcaaaaccac accgtcaaca ggttttttgt gtatttttca cgcctcacgg gcagcctcag
     6781 accttttgtg ttaaaacagt aatatgttct gctgttacaa tacggtacac acaggagaca
     6841 ctgtcacttt tatggcatgg cacacagttc aagagacact gaaattaaca ggaaagtcgc
     6901 gctcgcaatt atatcgtgac atggctaaag gccttgtcag ctatcgcaca ggaaaaaatg
     6961 atcgccggga gttcgagacc agtgaactga ttcgggccta tggcgagctg cgcacttctg
     7021 agacaccaaa aaggcacaag gagggacact ccaaatctgt cgttagccag gcggaaaatg
     7081 ctcagctaga cgtcattcag cagaaactgg acgctctgca gcagactgtt accctaatgc
     7141 ttgaagacaa gatggcgcgg gaagcagaaa accgcctgcg aaatgaggag agggaacaac
     7201 tacaggccga aattagccgt ctggctgacg cgctggcaca ggaaaaaaag cggggattct
     7261 ggtcgcggct gtttcgctcc ggttcagagt aacagtgcag gtgactctga tttaccataa
     7321 catctgttat cagcaccgcc tgagaggccc tcagggcgtt tctgacggga aaccggtaaa
     7381 acaggcaaca aaccacccga aaacccgcca gaaacgcgca ggctctgttt ttacggcatg
     7441 catgagtatg cgcacaagtg catagcgcgg aaattcacac cattcagccg gggtaagtcc
     7501 tgtttccggc gttcttccca tccgaaaacg gtgaacgggg acgcaattta ttgcgtggcc
     7561 gtgaagcgtt gccggcgcag ccggaaaacc cgctgtcagc acgttcagga agggggtgat
     7621 gtacaaatgg cacagaggat gaaagtgacg tatgagctga aggaatgatg taaaagtgat
     7681 gtataagtga tgtataagtg atgtataagt gatgtaaaag tgatgtatga actgaaggag
     7741 cgatgtaatt ctgtagtcag gaagaataac ccgttcatgc aggcatgaac gatacagtga
     7801 ggaacgcttt ttttgtcacg atctcccctt aacctgcgta aatcacccgc catggttgat
     7861 tatttttcaa cctcaaaatg gtcatacacc taacgcagcc gcgattttca tcgcaactgc
     7921 atgaattaaa agaaaaaaaa ggaatatttc gttttcacga gtaatacccg ggtattaaaa
     7981 atccactgta attacatgaa aataaagaaa taaaaggtaa taccggagta ttaccacgta
     8041 cgcgggcaag cgaaaaagct ataacgcttc ccggccgaca ggcgacccaa aatccacagg
     8101 ggcatgcagg ttctcttcag taacctgcgc cattaaatca aaaaggtcat attcgccggg
     8161 aagctgcgta tcagtcgaat ccagttgttc atcatattca gatggcatgg ggtgatatcc
     8221 agtagctgag aggttgttag cgggtttcgg ggcgcagccc tgaaccagtc atgtagcgct
     8281 agcggagtgt atactggctt actatgttgc cacgattgcg aaaatcagtg aagtgcttca
     8341 tgtggcagga gaaaaaaggc agcaccggcg cgtcagcaga atatgcgata caggagatat
     8401 tccgcttcct cgctcactga ctcgctacgc tcggtcgtt
//
LOCUS       DAKISA010000351         6716 bp    DNA     linear   BCT 29-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei strain R93
            SAMN28105251-rid15981353.guided.169, whole genome shotgun sequence.
ACCESSION   DAKISA010000351 DAKISA010000000
VERSION     DAKISA010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28105251
            Sequence Read Archive: SRR19126806
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001498512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001498512.1
            Genome Coverage       :: 261x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/26/2022 05:35:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 103 of 231
            Pseudo Genes (incomplete)         :: 75 of 231
            Pseudo Genes (internal stop)      :: 71 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6716
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28105251-rid15981353.guided.169"
                     /strain="R93"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="China: Sichuan"
                     /collection_date="2013"
                     /collected_by="shaofu Qiu"
     gene            <1..353
                     /locus_tag="OVW45_004684"
     CDS             <1..353
                     /locus_tag="OVW45_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="part of four member fumarate reductase enzyme
                     complex FrdABCD which catalyzes the reduction of fumarate
                     to succinate during anaerobic respiration; FrdAB are the
                     catalytic subcomplex consisting of a flavoprotein subunit
                     and an iron-sulfur subunit, respectively; FrdCD are the
                     membrane components which interact with quinone and are
                     involved in electron transfer; the catalytic subunits are
                     similar to succinate dehydrogenase SdhAB; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     flavoprotein subunit"
                     /protein_id="HCV5528920.1"
                     /translation="FKRVRITDTSSVFNTDLLYTIELGHGLNVAECMAHSAMARKESR
                     GAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEYSDVKITTLPPAKRVYGGEADAA
                     DKAEAANKKEKANG"
     gene            346..1080
                     /gene="frdB"
                     /locus_tag="OVW45_004685"
     CDS             346..1080
                     /gene="frdB"
                     /locus_tag="OVW45_004685"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCV5528921.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            1091..1486
                     /gene="frdC"
                     /locus_tag="OVW45_004686"
     CDS             1091..1486
                     /gene="frdC"
                     /locus_tag="OVW45_004686"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCV5528922.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            1497..1856
                     /gene="frdD"
                     /locus_tag="OVW45_004687"
     CDS             1497..1856
                     /gene="frdD"
                     /locus_tag="OVW45_004687"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCV5528923.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            1919..3052
                     /locus_tag="OVW45_004688"
     CDS             1919..3052
                     /locus_tag="OVW45_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCV5528924.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            3141..3674
                     /gene="blc"
                     /locus_tag="OVW45_004689"
     CDS             3141..3674
                     /gene="blc"
                     /locus_tag="OVW45_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCV5528925.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3671..3988)
                     /gene="sugE"
                     /locus_tag="OVW45_004690"
     CDS             complement(3671..3988)
                     /gene="sugE"
                     /locus_tag="OVW45_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCV5528926.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(4163..4309)
                     /gene="ecnB"
                     /locus_tag="OVW45_004691"
     CDS             complement(4163..4309)
                     /gene="ecnB"
                     /locus_tag="OVW45_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCV5528927.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(4420..4545)
                     /gene="ecnA"
                     /locus_tag="OVW45_004692"
     CDS             complement(4420..4545)
                     /gene="ecnA"
                     /locus_tag="OVW45_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCV5528928.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(4597..5163)
                     /gene="efp"
                     /locus_tag="OVW45_004693"
     CDS             complement(4597..5163)
                     /gene="efp"
                     /locus_tag="OVW45_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCV5528929.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            5205..6233
                     /gene="epmB"
                     /locus_tag="OVW45_004694"
     CDS             5205..6233
                     /gene="epmB"
                     /locus_tag="OVW45_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCV5528930.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            6623..>6716
                     /locus_tag="OVW45_004695"
     CDS             6623..>6716
                     /locus_tag="OVW45_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCV5528931.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRN"
BASE COUNT         1703 a         1703 c         1697 g         1613 t
ORIGIN      
        1 gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac ctgctctaca
       61 ccattgaact gggccacggt ctgaacgttg ctgaatgtat ggcgcactcc gcaatggcac
      121 gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag cgtgacgacg
      181 tcaacttcct caaacacacc ctcgccttcc gcgatgctga tggcacgact cgcctagagt
      241 acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt ggcgaagcgg
      301 atgcagccga taaggcggaa gcagccaata agaaggagaa ggcgaatggc tgagatgaaa
      361 aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc gcatagcgca
      421 ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg ctacatcaaa
      481 gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat ttgtggttcc
      541 tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct gcgtgattac
      601 accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga tctggtggtc
      661 gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat cggcaactcc
      721 cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa gtatcaccag
      781 ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt tggcctgaac
      841 ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga agatagccgc
      901 gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggcgt atggagctgt
      961 actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc tgcggccatt
     1021 cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa accacgctaa
     1081 ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt ccacctggtg
     1141 gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg ttccggctgt
     1201 gtggttcagc attgaactga ttttcgggct gtttgccctg aaaaatggcc cggaagcctg
     1261 ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc tgatcactct
     1321 ggcggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag cggccaatat
     1381 cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct gggcggtaac
     1441 tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag cctgagatga
     1501 ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc ggggccggtg
     1561 gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt ctgctgccac
     1621 tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc gcgcagagct
     1681 tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt ggtttacacc
     1741 gtatgcacca cgcgatgcac gatctgaaaa ttcacgtgcc tgcgggcaaa tgggttttct
     1801 acggtctggc tgctatcctg acagttgtca cgctgattgg tatcgttaca atctaacgta
     1861 tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac cagaccttat
     1921 gttcaaaacg acgctctgcg ccttattaat taccgcctct tgctccacat ttgctgcccc
     1981 tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc aacaaaagat
     2041 ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta cctggggcta
     2101 tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt taggttcggt
     2161 cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg aaatcaagtt
     2221 aagcgatccc gcaacaaaat actggcctga acttactgct aaacagtgga atgggatcac
     2281 actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc cggatgaagt
     2341 gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat gggctccagg
     2401 aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg ctgtgaagcc
     2461 gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac tcaaactcaa
     2521 ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg gatatcgcga
     2581 aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg gtgtgaagtc
     2641 gaccattgaa gatatggccc gctgggtgca aagcaattta accccccttg atatcaatga
     2701 gaaaacgctt caacaaggga tacaactggc acaatctcgc tactggcaaa ccggcgatat
     2761 gtatcagggc ctgggctggg aaatgctgga ctggccggtc aatcctgaca gcatcattaa
     2821 cggcagtgac aataaaattg cactggcagc acgcccggta aaagcgatta agcccccaac
     2881 tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat ttggtagcta
     2941 tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca aaaactatcc
     3001 caatccagcg agagtcgccg ccgcctgtca gattctcaac gctctacagt aaaatttcat
     3061 cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc tgtcatctac acttagaaaa
     3121 aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg acagctgcat
     3181 ttctggtcgt tgcctgcagt tctcctacgc cgccgcgtgg cgtgacagta gtgaataatt
     3241 tcgacgctaa acgctatctc ggtacctggt atgagattgc ccgttttgat caccgttttg
     3301 aacgtggact ggaaaaagtc accgcaacat acagtctgcg tgatgacggc ggcctgaatg
     3361 tcatcaataa aggctataac cctgacagag aaatgtggca gcagagtgaa gggaaagcgt
     3421 actttaccgg cgcaccaaca cgcgctgcgc tgaaagtgtc attctttggt cctttctatg
     3481 gcggttataa cgtcattgca cttgatcggg aataccgcca tgcgctggtt tgcggcccgg
     3541 accgcgacta cctgtggata ctctcccgca caccaaccat ttctgacgaa gtgaaacagg
     3601 agatgctggc agtcgcgacc cgggaagggt ttgatgtcag taagtttatt tgggtacagc
     3661 agcccggtag ttagtgagtg ctgagtttca gaccaataat ccccaatacg attaacgcca
     3721 gactcgccag gcgcatcggg ttagcggact caccgagcag cacaatgccg gtgatggccg
     3781 cgccgactgc gccaataccc gtccacacgg cataagccgt ccctaccggt aacgatttca
     3841 tcgcccaggc aagtagcgcc atactgacta tcatcgccgt cacagtaata acacttggcg
     3901 tcaaacgact aaagccgtgg gtatatttca ggccaacggc ccataccact tccagcagac
     3961 cagcaataac taagataatc caggacatat caggctccgg agaattgggg ccgtccccgg
     4021 tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg ttgaacggat attttgccag
     4081 gtggcagggg aatttcaact attacctttg tgactatcgc aaaaaaacaa acggcacgac
     4141 ataactgtcg taccgttatt gcttattgct gcgctttcgt tgctgcacca gaaatcgcgt
     4201 taccgccatc agaaatgtct tcaccaacgc cacgcgtggt gttgcaggca gttaatactg
     4261 ttgaaagcac cagaacagaa aagatcgctg caattgtctt cttcaccata acgtcttcct
     4321 tttaggtaat gttatttatg tttgcctata gcaaattaat aatagacaac atcgccaaaa
     4381 atgatggaaa tctgatgatt tttcggaaga gaagaaaaat tagctggcag cgcgggagat
     4441 ggagttgccg agatgtttga tgtcttcgcc gaaaccgcga gcggtgttac agcccgtgag
     4501 cagcgtgctg gcaagcaaaa caagaacgat aaggcgtttc atcatctctg acctgaataa
     4561 gtgatggtgc agcctgcagg ccgcaccaca accgcattac ttcacgcgag agacgtattc
     4621 accagagcgg gtatccactt tgatgacttc gccgatttgt acgaacagcg gaactttaac
     4681 cacagcgcca gtagacaggg tagccggttt gccgccagta cctgcggtat cacctttcag
     4741 gcccggatcg gtatcaacga tttccagttc aacgaagttc ggcggagtaa cggagatcgg
     4801 ctgaccattc cacagagtta cgatacactc tgcctgatcc agcagccatt tagcgttgtc
     4861 gccaattgct tttgcatcag cagacagctg ctcgaaagtt tcgttgttca tgaagtgcca
     4921 gaactcaccg tcgttgtaca ggtaagtcag gttcatatcg acaacatcag cgccttcagc
     4981 ggaatcagta gatttgaagg ttttttctac gcgagtaccg gtcagcagac gacgcagttt
     5041 aacgcgagca aatgcctggc ctttacccgg ttttacgaat tcactcgctt caaccgcgta
     5101 aggttcgccg tctaacatga ttttaagacc agcacgaaaa tcgttgctat agtacgttgc
     5161 cataaggccc tctgaaattt gttaattggt agctaagcca caaaatggcg catattgtaa
     5221 ccctaaatac cccatccaga gaagattggt taacgcaact tgccgatgtt gtgaccgatc
     5281 ctggtgaact tctgcgtctt ttgaatatag acgcggacga aaaactgttg gccggacgca
     5341 gcgccaaaaa gctgtttgcc ctgcgtgtgc cccgctcatt tatcgatcgc atggagaaag
     5401 gcaatccgga cgatcctatt ttgcgtcagg tacttacctc gcaagatgag tttgtcgtcg
     5461 cgtccggatt ctccaccgac ccactggaag aacagcacag cgtagtgcct ggtttgttgc
     5521 ataaatacca caaccgggcg cttttgctgg tcaaaggcgg ctgcgcggta aattgccgct
     5581 attgcttccg tcgccacttc ccctatgccg aaaatcaggg caacaagcgt aactggcaga
     5641 ctgcacttga gtatgttgct gcgcatccgg aactggacga gatgattttc tccggcggcg
     5701 atccgctgat ggcgaaagat cacgagctgg actggttgct cacacaactg gaagccatcc
     5761 cgcatatcaa acgtctgcgg attcacagcc gtctgccgat tgtgatcccg gcacgtatca
     5821 ccgaggcgct ggttgaacgc tttgcccgtt ctacgctgca aatcttgctg gtgaatcaca
     5881 tcaatcatgc caatgaggta gatgaaacgt tccgtcaggc gatggctaaa ttgcgccgtg
     5941 tcggtgtcac cctgcttaac cagagcgttc tgttacgtgg tgtgaacgat aacgcacaaa
     6001 cgttggcaaa cctgagtaat gcgttgttcg atgccggcgt gatgccgtat tacctgcatg
     6061 tgctagataa agtacagggc gcggcgcatt ttatggtgag tgatgacgaa gcacggcaga
     6121 ttatgcgtga gttgctgaca ttggtgtcgg gatatctggt gccgaaactg gcgcgagaaa
     6181 ttggcggcga acccagcaaa acgccgctgg atctccagct acgccagcag taactgatta
     6241 aacagggaag ttaaacatta gcctgatgcg ctacgcttat caggcctaca ttgtgcattg
     6301 aaattgaatg gatttgcaat gaactgtagg ccggataagg cgtttacgcc gcatccggca
     6361 agagcaacaa acgcaagtac agcaacctgt cattaaactg ggaaatttaa tctttgcccg
     6421 ttgcataacc tttaattatt catatacatt gctgttgtta ctctttatat agccaacacc
     6481 aataattaac ctttcttttc tgtttatata gagtgagtac ggctatttat gttattttaa
     6541 caactattac taccaagccc atttatctgg cttataaatt aaaattaatt atttaattga
     6601 gaaatggtta gggagaacct acatggccat aagcatcaag ggtgttaata ccggtgttat
     6661 tcgcaagagt aataatttca ttgcactggc gctgaaaatt aaagaacctc gcaata
//
